LOCUS       DAMZFT010000001       119073 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.001, whole genome shotgun sequence.
ACCESSION   DAMZFT010000001 DAMZFT010000000
VERSION     DAMZFT010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 119073)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 119073)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..119073
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.001"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(35..766)
                     /gene="rsmE"
                     /locus_tag="PN977_000001"
     CDS             complement(35..766)
                     /gene="rsmE"
                     /locus_tag="PN977_000001"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003853382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HDI6040091.1"
                     /translation="MRIPRIYHPEPLTSHSHIALCEDAANHIGRVLRMGPGQALQLFD
                     GSNQVFDAEITSASKKSVEVKVLEGQIDDRESPLHIHLGQVMSRGEKMEFTIQKSIEL
                     GVSLITPLFSERCGVKLDSERLNKKLQQWQKIAIAACEQCGRNRVPEIRPAMDLEAWC
                     AEQDEGLKLNLHPRASNSINTLPLPVERVRLLIGPEGGLSADEIAMTARYQFTDILLG
                     PRVLRTETTALTAITALQVRFGDLG"
     gene            complement(846..1553)
                     /gene="endA"
                     /locus_tag="PN977_000002"
     CDS             complement(846..1553)
                     /gene="endA"
                     /locus_tag="PN977_000002"
                     /EC_number="3.1.21.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease I"
                     /protein_id="HDI6040092.1"
                     /translation="MYRYLSIAAVVLSAAFSGPALAEGINSFSQAKAAAVKVHADAPG
                     TFYCGCKINWQGKKGVVDLQSCGYQVRKNENRASRVEWEHVVPAWQFGHQRQCWQDGG
                     RKNCAKDPVYRKMESDMHNLQPSVGEVNGDRGNFMYSQWNGGEGQYGQCAMKVDFKEK
                     AAEPPARARGAIARTYFYMRDQYNLTLSRQQTQLFNAWNKMYPVTDWECERDERIAKV
                     QGNHNPYVQRACQARKS"
     gene            complement(1648..2145)
                     /locus_tag="PN977_000003"
     CDS             complement(1648..2145)
                     /locus_tag="PN977_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HDI6040093.1"
                     /translation="MKTSRLPIAIQQAVMRRLREKLAQANLKLGRNYPEPKLSYTQRG
                     TSAGTAWLESYEIRLNPVLLLENSEAFIEEVVPHELAHLLVWKHFGRVAPHGKEWKWM
                     MENVLGVPARRTHQFELQSVRRNTFPYRCKCQEHQLTVRRHNRVVRGEAVYRCVHCGE
                     QLVAK"
     gene            complement(2222..3616)
                     /gene="galP"
                     /locus_tag="PN977_000004"
     CDS             complement(2222..3616)
                     /gene="galP"
                     /locus_tag="PN977_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/proton symporter"
                     /protein_id="HDI6040094.1"
                     /translation="MPDAKKQGRSNKAMTFFVCFLAALAGLLFGLDIGVIAGALPFIA
                     DEFQITSHTQEWVVSSMMFGAAVGAVGSGWLSFKLGRKKSLMIGAILFVAGSLFSAAA
                     PNVEVLILSRVLLGLAVGVASYTAPLYLSEIAPEKIRGSMISMYQLMITIGILGAYLS
                     DTAFSYTGAWRWMLGVIIIPAILLLIGVFFLPDSPRWFAAKRRFVDAERVLLRLRDTS
                     AEAKRELDEIRESLQVKQSGWALFKENSNFRRAVFLGVLLQVMQQFTGMNVIMYYAPK
                     IFELAGYTNTTEQMWGTVIVGLTNVLATFIAIGLVDRWGRKPTLTLGFLVMAAGMGVL
                     GTMMHIGIHSPSAQYFAIAMLLMFIVGFAMSAGPLIWVLCSEIQPLKGRDFGITCSTA
                     TNWIANMIVGATFLTMLNTLGNANTFWVYAALNVLFILLTLWLVPETKHVSLEHIERN
                     LMKGRKLREIGAHD"
     gene            complement(4040..5194)
                     /gene="metK"
                     /locus_tag="PN977_000005"
     CDS             complement(4040..5194)
                     /gene="metK"
                     /locus_tag="PN977_000005"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HDI6040095.1"
                     /translation="MAKHLFTSESVSEGHPDKIADQISDAVLDAILEQDPKARVACET
                     YVKTGMVLVGGEITTSAWVDIEEITRNTVREIGYVHSDMGFDANSCAVLSAIGKQSPD
                     INQGVDRADPLEQGAGDQGLMFGYATNETDVLMPAPITYAHRLVQRQAEVRKNGTLPW
                     LRPDAKSQVTFQYDDGKIVGIDAVVLSTQHSEEIDQKSLQEAVMEEIIKPILPAEWLT
                     SATKFFINPTGRFVIGGPMGDCGLTGRKIIVDTYGGMARHGGGAFSGKDPSKVDRSAA
                     YAARYVAKNIVAAGLADRCEIQVSYAIGVAEPTSIMVETFGTEKVPSEQLTLLVREFF
                     DLRPYGLIQMLDLLHPIYKETAAYGHFGREHFPWEKTDKAQLLRDAAGLK"
     gene            complement(5498..5713)
                     /locus_tag="PN977_000006"
     CDS             complement(5498..5713)
                     /locus_tag="PN977_000006"
                     /inference="COORDINATES: protein motif:HMM:NF037550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6040096.1"
                     /translation="MGITSAGMQSRDAECGERIFTRTVRQVKQQTTVHYFVSPPRPPV
                     KTNPQAKTLISTRLEVATRKKRRVLFI"
     gene            5849..5980
                     /gene="yqgB"
                     /locus_tag="PN977_000007"
     CDS             5849..5980
                     /gene="yqgB"
                     /locus_tag="PN977_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response protein YqgB"
                     /protein_id="HDI6040097.1"
                     /translation="MKKKPVAQLERQHSLLENPCAYGLLSQFQAAIVVNCFTLNKII"
     gene            5989..7965
                     /gene="speA"
                     /locus_tag="PN977_000008"
     CDS             5989..7965
                     /gene="speA"
                     /locus_tag="PN977_000008"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic arginine decarboxylase"
                     /protein_id="HDI6040098.1"
                     /translation="MSDDMSMGLPSSAGEHGVLRSMQEVAMSSQEASKMLRTYNIAWW
                     GNNYYDVNELGHISVCPDPDVPEARVDLAQLVKTREAQGQRLPALFCFPQILQHRLRS
                     INAAFKRARESYGYNGDYFLVYPIKVNQHRRVIESLIHSGEPLGLEAGSKAELMAVLA
                     HAGMTRSVIVCNGYKDREYIRLALIGEKMGHKVYLVIEKMSEIAIVLDEAERLNVVPR
                     LGVRARLASQGSGKWQSSGGEKSKFGLAATQVLQLVETLREAGRLDSLQLLHFHLGSQ
                     MANIRDIATGVRESARFYVELHKLGVNIQCFDVGGGLGVDYEGTRSQSDCSVNYGLNE
                     YANNIIWAIGDACEENGLPHPTVITESGRAVTAHHTVLVSNIIGVERNEYTVPTAPAE
                     DAPRALQSMWETWQEMHEPGTRRSLREWLHDSQMDLHDIHIGYSSGIFSLQERAWAEQ
                     LYLSMCHEVQKQLDPQNRAHRPIIDELQERMADKMYVNFSLFQSMPDAWGIDQLFPVL
                     PLEGLDQVPERRAVLLDITCDSDGAIDHYIDGDGIATTMPMPEYDPENPPMLGFFMVG
                     AYQEILGNMHNLFGDTEAVDVFVFPDGSVEVELSDEGDTVADMLQYVQLDPKTLLTQF
                     RDQVKKTDLDAELQQQFLEEFEAGLYGYTYLEDE"
     gene            8103..9023
                     /gene="speB"
                     /locus_tag="PN977_000009"
     CDS             8103..9023
                     /gene="speB"
                     /locus_tag="PN977_000009"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HDI6040099.1"
                     /translation="MSTLGHQYDNSLVSNAFGFLRLPMNFQPYDSDADWVITGVPFDM
                     ATSGRAGGRHGPAAIRQVSTNLAWEHNRFPWNFDMRERLNVVDCGDLVYAFGDAREMS
                     EKLQAHAEKLLAAGKRMLSFGGDHFVTLPLLRAHAKHFGKMALVHFDAHTDTYANGCE
                     FDHGTMFYTAPKEGLIDPNHSVQIGIRTEFDKDNGFTVLDACQVNDRSVDDVIAQVKQ
                     IVGDMPVYLTFDIDCLDPAFAPGTGTPVIGGLTSDRAIKLVRGLKDLNIVGMDVVEVA
                     PAYDQSEITALAAATLALEMLYIQAAKKGE"
     gene            complement(9229..9987)
                     /gene="loiP"
                     /locus_tag="PN977_000010"
                     /gene_synonym="yggG"
     CDS             complement(9229..9987)
                     /gene="loiP"
                     /locus_tag="PN977_000010"
                     /gene_synonym="yggG"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417411.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease LoiP"
                     /protein_id="HDI6040100.1"
                     /translation="MKIRALLVAMSVATVLTGCQNMDSNGLLSSGAEAFQAYSLSDAQ
                     VKTLSDQACQEMDSKATIAPANSEYAKRLTTIANALGNNINGQPVNYKVYMAKDVNAF
                     AMANGCIRVYSGLMDMMTDNEVEAVIGHEMGHVALGHVKKGMQVALGTNAVRVAAASA
                     GGIVGSLSQSQLGNLGEKLVNSQFSQRQEAEADDYSYDLLRQRGISPAGLATSFEKLA
                     KLEEGRQSSMFDDHPASAERAQHIRDRMSADGIK"
     gene            10265..12256
                     /gene="tkt"
                     /locus_tag="PN977_000011"
     CDS             10265..12256
                     /gene="tkt"
                     /locus_tag="PN977_000011"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HDI6040101.1"
                     /translation="MSSRKELANAIRALSMDAVQKAKSGHPGAPMGMADIAEVLWRDF
                     LKHNPQNPSWADRDRFVLSNGHGSMLIYSLLHLTGYDLPMEELKNFRQLHSKTPGHPE
                     VGYTAGVETTTGPLGQGIANAVGMAIAEKTLAAQFNRPGHDIVDHYTYAFMGDGCMME
                     GISHEVCSLAGTLKLGKLIAFYDDNGISIDGHVEGWFTDDTAMRFEAYGWHVIRDIDG
                     HDAASIKRAVEEARAVTDKPSLLMCKTIIGFGSPNKAGTHDSHGAPLGDAEIALTREQ
                     LGWKYAPFEIPSEIYAQWDAKEAGQAKESAWNEKFAAYAKAYPQEAAEFTRRMKGEMP
                     SDFDAKAKEFIAKLQANPAKIASRKASQNAIEAFGPLLPEFLGGSADLAPSNLTLWSG
                     SKAINEDAAGNYIHYGVREFGMTAIANGISLHGGFLPYTSTFLMFVEYARNAVRMAAL
                     MKQRQVMVYTHDSIGLGEDGPTHQPVEQVASLRVTPNMSTWRPCDQVESAVAWKYGVE
                     RQDGPTALILSRQNLAQQERTEEQLANIARGGYVLKDCAGQPELIFIATGSEVELAVA
                     AYEKLTAEGVKARVVSMPSTDAFDKQDAAYRESVLPKAVTARVAVEAGIADYWYKYVG
                     LNGAIVGMTTFGESAPAELLFEEFGFTVDNVVAKAKELL"
     gene            12570..13013
                     /gene="cmtB"
                     /locus_tag="PN977_000012"
     CDS             12570..13013
                     /gene="cmtB"
                     /locus_tag="PN977_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IIA"
                     /protein_id="HDI6040102.1"
                     /translation="MRLSDYFPESSISVIHSAKDWQEAIDFSMVSLLDKNYISENYIQ
                     AIKDSTINNGPYYILAPGVAMPHARPECGALKTGMSLTLLEQGVYFPGNDEPIKLLIG
                     LSAADADSHIGAIQALSELLCEEEILEQLLTASSEKQLADIISRG"
     gene            13041..14429
                     /gene="cmtA"
                     /locus_tag="PN977_000013"
     CDS             13041..14429
                     /gene="cmtA"
                     /locus_tag="PN977_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICB"
                     /protein_id="HDI6040103.1"
                     /translation="MENKSARAKVQAFGGFLTAMVIPNIGAFIAWGFITALFIPTGWL
                     PNEHFAKIVGPMITYLLPVMIGSTGGHLVGGKRGAVMGGIGTIGVIVGAEIPMFLGSM
                     IMGPLGGLVIKYVDKALEKRIPAGFEMVINNFSLGIAGMLLCLLGFEVIGPAVLIANT
                     FVKECIEALVHAGYLPLLSVINEPAKVLFLNNAIDQGVYYPLGMQQASVNGKSIFFMV
                     ASNPGPGLGLLLAFTLFGKGMSKRSAPGAMIIHFLGGIHELYFPYVLMKPLTIIAMIA
                     GGMSGTWMFNLLDGGLVAGPSPGSIFAYLALTPKGSFLATIAGVTVGTLVSFAITSLI
                     LKMEKTVETESEDEFAQSANAVKAMKQEGAFSLSRVKRIAFVCDAGMGSSAMGATTFR
                     KRLEKAGLAIEVKHYAIENVPADADIVVTHASLEGRVKRVTDKPLILINNYIGDPKLD
                     TLFNQLTAEHKH"
     gene            14444..15721
                     /locus_tag="PN977_000014"
     CDS             14444..15721
                     /locus_tag="PN977_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HDI6040104.1"
                     /translation="MKTKVAAIYGKRDVRLRVFELPEITDNELLVSVISDSVCLSTWK
                     AALLGSEHKRVPDDLENHPVITGHECAGVIVEVGKNLTGKYKKGQRFVLQPAMGLPSG
                     YSAGYSYEYFGGNATYMIIPEIAINLGCVLPYHGSYFAAASLAEPMCCIIGAYHANYH
                     TTQYVYEHRMGVKPGGNIALLACAGPMGIGAIDYAINGGIQPSRVVVVDIDDKRLAQV
                     QKLLPVELAASKGIELVYVNTKGMSDPVQMLRALTGDAGFDDIFVYAAVPAVVEMADE
                     LLAEDGCLNFFAGPTDKNFKVPFNFYNVHYNSTHVVGTSGGSTDDMKEAIALSATGQL
                     QPSFMVTHIGGLDAVPETVLNLPDIPGGKKLIYNGVTMPLTAIADFAEKGKTDPLFKE
                     LARLVEETHGIWNEQAEKYLLAQFGVDIGEAAQ"
     gene            15718..16683
                     /gene="yggF"
                     /locus_tag="PN977_000015"
     CDS             15718..16683
                     /gene="yggF"
                     /locus_tag="PN977_000015"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HDI6040105.1"
                     /translation="MMSLAWPLFRVTEQAALAAWPQTGCGDKNKIDGLAVTAMRQALN
                     DVAFRGRVVIGEGEIDHAPMLWIGEEVGKGDGPEVDIAVDPIEGTRMVAMGQSNALAV
                     MAFAPRDSLLHAPDMYMKKLVVNRLAAGAIDLSLPLTDNLRNVAKALGKPLDKLRMVT
                     LDKPRLSAAIEEATQLGVKVFALPDGDVAASVLTCWQDNPYDVMYTIGGAPEGVISAC
                     AVKALGGDMQAELIDFCQAKGDYTENRQIAEQERKRCKAMGVDVNRVYSLDELVRGND
                     ILFSATGVTGGELVNGIQQTANGVRTQTLLIGGADQTCNIIDSLH"
     gene            16705..17214
                     /gene="fumE"
                     /locus_tag="PN977_000016"
     CDS             16705..17214
                     /gene="fumE"
                     /locus_tag="PN977_000016"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarase E"
                     /protein_id="HDI6040106.1"
                     /translation="MATLTEDDVLEQLDAQDNLFSFMKTAHTILLQGIRQFLPSLFVD
                     NDEEIVEYAVKPLLAQSGPLDDIDVALRLIYALGKMDKWLYADITHFSQFWHYLNEQD
                     ETPGFADDMTWDFISNVNSITRNAMLYDALKAMKFADFSVWSEARFSGMVKTALTLAV
                     TTTLKELTP"
     gene            17211..17924
                     /locus_tag="PN977_000017"
     CDS             17211..17924
                     /locus_tag="PN977_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside/nucleotide kinase family protein"
                     /protein_id="HDI6040107.1"
                     /translation="MKIELTVNGLKIQAQYQNEEIENVHKPLLHMLAALQTVNPQRRT
                     VVFLCAPPGTGKSTLTTFWEYLAQQDPELPAIQTLPMDGFHHYNSWLDAHQLRPFKGA
                     PETFDVAKLTENLRQVVEGDCTWPQYDRQKHDPVEDALHVTAPLVIVEGNWLLLDDEK
                     WLELASFCDFSIFIHAPAQILRERLISRKIAGGLTRQVAEAFYARTDGPNVERVLMNS
                     RQANLIVEMTEEGRYHFTS"
     gene            18209..19228
                     /gene="epd"
                     /locus_tag="PN977_000018"
     CDS             18209..19228
                     /gene="epd"
                     /locus_tag="PN977_000018"
                     /EC_number="1.2.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="erythrose-4-phosphate dehydrogenase"
                     /protein_id="HDI6040108.1"
                     /translation="MTVRVAINGFGRIGRNVVRALYESGRRAEITVVAINELADAAGM
                     AHLLKYDTSHGRFAWEVRQERDQLFVGDDAIRVLHERSLQSLPWRELGVDVVLDCTGV
                     YGSREHGEAHIAAGAKKVLFSHPGSNDLDATVVYGVNQDQLRAEHRIVSNASCTTNCI
                     IPVIKLLDDAYGIESGTVTTIHSAMHDQQVIDAYHPDLRRTRAASQSIIPVDTKLAAG
                     ITRFFPQFNDRFEAIAVRVPTINVTAIDLSVTVKKPVKANEVNLLLQKAAQGAFHGIV
                     DYTELPLVSVDFNHDPHSAIVDGTQTRVSGAHLIKTLVWCDNEWGFANRMLDTTLAMA
                     TVAFR"
     gene            19278..20441
                     /gene="pgk"
                     /locus_tag="PN977_000019"
     CDS             19278..20441
                     /gene="pgk"
                     /locus_tag="PN977_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HDI6040109.1"
                     /translation="MSVIKMTDLDLAGKRVFIRADLNVPVKDGKVTSDARIRASLPTI
                     ELALKQGAKVMVTSHLGRPTEGEYNEEFSLLPVVNYLKDKLSNPVRLVKDYLDGVDVA
                     EGELVVLENVRFNKGEKKDDETLSKKYAALCDVFVMDAFGTAHRAQASTHGIGKFADV
                     ACAGPLLAAELDALGKALKEPARPMVAIVGGSKVSTKLTVLDSLSKIADQLIVGGGIA
                     NTFIAAQGHDVGKSLYEADLVDEAKRLLTTCNIPVPSDVRVATEFSETAPATLKSVND
                     VKADEQILDIGDASAQELAEILKNAKTILWNGPVGVFEFPNFRKGTEIVANAIADSEA
                     FSIAGGGDTLAAIDLFGIADKISYISTGGGAFLEFVEGKVLPAVAMLEERAKK"
     gene            20656..21735
                     /gene="fbaA"
                     /locus_tag="PN977_000020"
     CDS             20656..21735
                     /gene="fbaA"
                     /locus_tag="PN977_000020"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016505616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HDI6040110.1"
                     /translation="MSKIFDFVKPGVITGDDVQKVFQVAKENNFALPAVNCVGTDSIN
                     AVLETAAKVKAPVIVQFSNGGASFIAGKGVKSDVPQGAAILGAISGAHHVHQMAEHYG
                     VPVILHTDHCAKKLLPWIDGLLDAGEKHFAATGKPLFSSHMIDLSEESLQENIEICSK
                     YLERMSKIGMTLEIELGCTGGEEDGVDNSHMDASALYTQPEDVDYAYTELSKISPRFT
                     IAASFGNVHGVYKPGNVVLTPTILRDSQEYVSKKHNLPHNSLNFVFHGGSGSTAQEIK
                     DSVSYGVVKMNIDTDTQWATWEGVLNYYKANEAYLQGQLGNPKGEDQPNKKYYDPRVW
                     LRAGQTSMIARLEKAFQELNAIDVL"
     gene            22093..22953
                     /gene="mscS"
                     /locus_tag="PN977_000021"
     CDS             22093..22953
                     /gene="mscS"
                     /locus_tag="PN977_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small-conductance mechanosensitive channel MscS"
                     /protein_id="HDI6040111.1"
                     /translation="MEDLNVVDSINGAGSWLVANQALLLSYAVNIVAALAIIIVGLII
                     ARMISNAVNRLMISRKIDATVADFLSALVRYGIIAFTLIAALGRVGVQTASVIAVLGA
                     AGLAVGLALQGSLSNLAAGVLLVMFRPFRAGEYVDLGGVAGTVLSVQIFSTTMRTADG
                     KIIVIPNGKIIAGNIINFSREPVRRNEFIIGVAYDSDIDQVKQILTNIIQSEDRILKD
                     REMTVRLNELGASSINFVVRVWSNSGDLQNVYWDVLERIKREFDAAGISFPYPQMDVN
                     FKRVKEDKAA"
     gene            23092..23727
                     /gene="argO"
                     /locus_tag="PN977_000022"
     CDS             23092..23727
                     /gene="argO"
                     /locus_tag="PN977_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine exporter ArgO"
                     /protein_id="HDI6040112.1"
                     /translation="MFSYYFQGLALGAAMILPLGPQNAFVMNQGIRRQYHIMIALLCA
                     ISDLVLICAGIFGGSALLMQSPWLLALVTWGGVAFLLWYGFGAFKTAMSSNIELASAE
                     VMKQGRWKIIATMLAVTWLNPHVYLDTFVVLGSLGGQLDVEPKRWFALGTISASFLWF
                     FGLALLAAWLAPRLRTAKAQRIINLVVGCVMWFIALQLARDGIAHAQALFS"
     gene            23820..24560
                     /locus_tag="PN977_000023"
     CDS             23820..24560
                     /locus_tag="PN977_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative stress defense protein"
                     /protein_id="HDI6040113.1"
                     /translation="MKFKVIALAALMGISGMAAQANELPDGPHIVTSGTASVDAVPDI
                     ATLAIEVNVAAKDAATAKKQADERVAQYISFLELNQIAKKDISSANLRTQPDYDYQDG
                     KSILKGYRAVRTVEVTLRQLDKLNSLLDGALKAGLNEIRSVSLGVAQPDAYKDKARKA
                     AIDNAIHQAQELANGFHRKLGPVYSVRYHVSNYQPSPMVRMMKADAAPVSAQETYEQA
                     AIQFDDQVDVVFQLEPVDQQPAKTPAAQ"
     gene            24727..25623
                     /locus_tag="PN977_000024"
                     /pseudo
     CDS             24727..25623
                     /locus_tag="PN977_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417396.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            complement(25620..27098)
                     /gene="scpC"
                     /locus_tag="PN977_000025"
     CDS             complement(25620..27098)
                     /gene="scpC"
                     /locus_tag="PN977_000025"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionyl-CoA:succinate CoA transferase"
                     /protein_id="HDI6040114.1"
                     /translation="METQWTRMTANEAAEIIQHNDMVAFSGFTPAGSPKALPTAIARR
                     ANEQHEAKKPYQIRLLTGASISAAADDVLSDADAVSWRAPYQTSSGLRKKINQGAVSF
                     VDLHLSEVAQMVNYGFFGDIDVAVIEASALAPDGRVWLTSGIGNAPTWLLRAKKVIIE
                     LNHYHDPRVAELADIVIPGAPPRRNSVSIFHAMDRVGTRYVQIDPKKIVAVVETNLPD
                     AGNMLDKQNPMCQQIADNVVTFLLQEMAHGRIPPEFLPLQSGVGNINNAVMARLGENP
                     VIPPFMMYSEVLQESVVHLLETGKISGASASSLTISADSLRKIYDNMDYFASRIVLRP
                     QEISNNPEIIRRLGVIALNVGLEFDIYGHANSTHVAGVDLMNGIGGSGDFERNAYLSI
                     FMAPSIAKEGKISTVVPMCSHVDHSEHSVKVIITEQGIADLRGLSPLQRARTIIDNCA
                     HPMYRDYLHRYLENAPGGHIHHDLSHVFDLHRNLIATGSMLG"
     gene            complement(27122..27907)
                     /gene="scpB"
                     /locus_tag="PN977_000026"
     CDS             complement(27122..27907)
                     /gene="scpB"
                     /locus_tag="PN977_000026"
                     /EC_number="7.2.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA decarboxylase"
                     /protein_id="HDI6040115.1"
                     /translation="MSYQYVNVVTINKVAVIEFNYGRKLNALSKVFIDDLMQALSDLN
                     RPEIRCIILRAPSGSKVFSAGHDIHELPSGGRDPLSYDDPLRQITRMIQKFPKPIISM
                     VEGSVWGGAFEMIMSSDLIIAASTSTFSMTPVNLGVPYNLVGIHNLTRDAGFHIVKEL
                     IFTASPITAQRALAVGILNHVVEVEELEDFTLQMAHHISEKAPLAIAVIKEELRVLGE
                     AHTMNSDEFERIQGMRRAVYDSEDYQEGMNAFLEKRKPNFVGH"
     gene            complement(27918..28913)
                     /gene="meaB"
                     /locus_tag="PN977_000027"
     CDS             complement(27918..28913)
                     /gene="meaB"
                     /locus_tag="PN977_000027"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HDI6040116.1"
                     /translation="MINEATLAESIRRLRQGERATLAQAMTLVESRHPRHQALSTQLL
                     DAIMPYCGNTLRLGVTGTPGAGKSTFLEAFGMLLIREGLKVAVIAVDPSSPVTGGSIL
                     GDKTRMNDLARAEAAFIRPVPSSGHLGGASQRARELMLLCEAAGYDVVIVETVGVGQS
                     ETEVARMVDCFISLQIAGGGDDLQGIKKGLMEVADLIVINKDDGDNHTNVAIARHMYE
                     SALHILRRKYDEWQPRVLTCSALEKRGIDEIWHAIIDFKTALTASGRLQQVRQQQSVE
                     WLRKQTEEEVLNHLFANEDFDRYYRQTLLAVKNNTLSPRTGLRQLSEFIQTQYFD"
     gene            complement(28906..31050)
                     /gene="scpA"
                     /locus_tag="PN977_000028"
     CDS             complement(28906..31050)
                     /gene="scpA"
                     /locus_tag="PN977_000028"
                     /EC_number="5.4.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase"
                     /protein_id="HDI6040117.1"
                     /translation="MSNVQEWQQLANKELSRREKTVDSLVHQTAEGIAIKPLYTEADL
                     DNLEVTGTLPGLPPYVRGPRATMYTAQPWTIRQYAGFSTAKESNAFYRRNLAAGQKGL
                     SVAFDLATHRGYDSDNPRVAGDVGKAGVAIDTVEDMKVLFDQIPLDKMSVSMTMNGAV
                     LPVLAFYIVAAEEQGVTPDKLTGTIQNDILKEYLCRNTYIYPPKPSMRIIADIIAWCS
                     GNMPRFNTISISGYHMGEAGANCVQQVAFTLADGIEYIKAAISAGLKIDDFAPRLSFF
                     FGIGMDLFMNVAMLRAARYLWSEAVSGFGAQDPKSLALRTHCQTSGWSLTEQDPYNNV
                     IRTTIEALAATLGGTQSLHTNAFDEALGLPTDFSARIARNTQIIIQEESELCRTVDPL
                     AGSYYIESLTDQIVKQARAIIQQIDEAGGMAKAIEAGLPKRMIEEASAREQSLIDQGK
                     RVIVGVNKYKLDHEDETDVLEIDNVMVRNEQIASLERIRATRDDAAVTAALNALTHAA
                     QHNENLLAAAVNAARVRATLGEISDALEVAFDRYLVPSQCVTGVIAQSYHQSEKSASE
                     FDAIVAQTEQFLADNGRRPRILIAKMGQDGHDRGAKVIASAYSDLGFDVDLSPMFSTP
                     EEIARLAVENDVHVVGASSLAAGHKTLIPELVEALKKWGREDICVVAGGVIPPQDYAF
                     LQERGVAAIYGPGTPMLDSVRDVLNLISQHHD"
     gene            complement(31254..32147)
                     /gene="argP"
                     /locus_tag="PN977_000029"
     CDS             complement(31254..32147)
                     /gene="argP"
                     /locus_tag="PN977_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator ArgP"
                     /protein_id="HDI6040118.1"
                     /translation="MKRPDYRTLQALDAVIRERGFERAAQKLCITQSAVSQRIKQLEN
                     MFGQPLLVRTVPPRPTEQGQKLLALLRQVELLEEEWLGDEQTGSTPLLLSLAVNADSL
                     ATWLLPALAPVLADSPIRLNLQVEDETRTQERLRRGEVVGAVSIQHQALPSCLVDKLG
                     ALDYLFVSSKPFAEKYFPNGVTRSALLKAPVVAFDHLDDMHQAFLQQNFDLPPGSVPC
                     HIVNSSEAFVQLARQGTTCCMIPHLQIEKELASGELIDLTPGLFQRRMLYWHRFAPES
                     RMMRKVTDALLDYGHKVLRQD"
     gene            32289..32519
                     /locus_tag="PN977_000030"
     CDS             32289..32519
                     /locus_tag="PN977_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001328908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6040119.1"
                     /translation="MHFAQRVRALVVLNGVALLPQFACKQGLANGELVRLFAPWSGIP
                     RPLYALFAGRKGMPAIARYFMDELTTRLANGV"
     gene            32575..33234
                     /gene="rpiA"
                     /locus_tag="PN977_000031"
     CDS             32575..33234
                     /gene="rpiA"
                     /locus_tag="PN977_000031"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HDI6040120.1"
                     /translation="MTQDELKKAVGWAALQYVQPGTIVGVGTGSTAAHFIDALGTMKG
                     QIEGAVSSSDASTEKLKSLGIHVFDLNEVDSLGIYVDGADEINGHMQMIKGGGAALTR
                     EKIIASVAEKFICIADASKQVDILGKFPLPVEVIPMARSAVARQLVKLGGRPEYRQGV
                     VTDNGNVILDVHGMEILDPIAMENAINAIPGVVTVGLFANRGADVALIGTPDGVKTIV
                     K"
     gene            33490..34722
                     /gene="serA"
                     /locus_tag="PN977_000032"
     CDS             33490..34722
                     /gene="serA"
                     /locus_tag="PN977_000032"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HDI6040121.1"
                     /translation="MAKVSLEKDKIKFLLVEGVHQKALESLRAAGYTNIEFHKGALDD
                     EQLKESIRDAHFIGLRSRTHLTEDVINAAEKLVAIGCFCIGTNQVDLDAAAKRGIPVF
                     NAPFSNTRSVAELVIGELLLLLRGVPEANAKAHRGVWNKLAAGSFEARGKKLGIIGYG
                     HIGTQLGILAESLGMYVYFYDIENKLPLGNATQVQHLSDLLNMSDVVSLHVPENPSTK
                     NMMGAKEISLMKPGSLLINASRGTVVDIPALCDALASKHLAGAAIDVFPTEPATNSDP
                     FTSPLCEFDNVLLTPHIGGSTQEAQENIGLEVAGKLIKYSDNGSTLSAVNFPEVSLPL
                     HGGRRLMHIHENRPGVLTALNKIFAEQGVNIAAQYLQTSAQMGYVVIDIEADEDVAEK
                     ALQAMKAIPGTIRARLLY"
     gene            34951..35010
                     /gene="ibsC"
                     /locus_tag="PN977_000033"
     CDS             34951..35010
                     /gene="ibsC"
                     /locus_tag="PN977_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsC"
                     /protein_id="HDI6040122.1"
                     /translation="MMRLVIILIVLLLISFSAY"
     gene            complement(35111..35713)
                     /locus_tag="PN977_000034"
     CDS             complement(35111..35713)
                     /locus_tag="PN977_000034"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HDI6040123.1"
                     /translation="MTQLPELPLTLSRQEIRKMIRQRRRALTPEQQQEMGQQAATRMM
                     TYPPVVMAHTVAVFLSFDGELDTQPLIEQLWRAGKRVYLPVLHPFSAGNLLFLNYHPQ
                     SELVMNRLKIHEPKLDVRDVLPLSRLDVLITPLVAFDEYGQRLGMGGGFYDRTLQNWQ
                     HYKTQPVGYAHDCQLVEKLPVEEWDIPLPAVVTPSKVWEW"
     gene            complement(35734..35917)
                     /gene="ssrS"
                     /locus_tag="PN977_000035"
     ncRNA           complement(35734..35917)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="PN977_000035"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(35959..36288)
                     /gene="zapA"
                     /locus_tag="PN977_000036"
     CDS             complement(35959..36288)
                     /gene="zapA"
                     /locus_tag="PN977_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HDI6040124.1"
                     /translation="MSAQPVDIQIFGRSLRVNCPPDQRDALNQAADDLNQRLQDLKER
                     TRVTNTEQLVFIAALNISYELAQEKAKTRDYAASMEQRIRMLQQTIEQALLEQGRITE
                     KTNQNFE"
     gene            36456..37034
                     /gene="ygfB"
                     /locus_tag="PN977_000037"
     CDS             36456..37034
                     /gene="ygfB"
                     /locus_tag="PN977_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YgfB"
                     /protein_id="HDI6040125.1"
                     /translation="MSIQNEMPGYNEMNQYLNQQGTGLTPAEMHGLISGMICGGNDDS
                     SWLPLLHDLTNEGMAFGHELAQALRKMHSATSDALQDDGFLFQLYLPDGDDVSVFDRA
                     DALAGWVNHFLLGLGVTQPKLDKVTGETGEAIDDLRNIAQLGYDEDEDQEELEMSLEE
                     IIEYVRVAALLCHDTFTHPQPTAPEVQKPTLH"
     gene            37060..38385
                     /gene="pepP"
                     /locus_tag="PN977_000038"
     CDS             37060..38385
                     /gene="pepP"
                     /locus_tag="PN977_000038"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro aminopeptidase"
                     /protein_id="HDI6040126.1"
                     /translation="MSEISRQEFQRRRQALVEQMQPGSAALIFAAPEVTRSADSEYPY
                     RQNSDFWYFTGFNEPEAVLVLIKSDDTHNHSVLFNRVRDLTAEIWFGRRLGQDAAPEK
                     LGVDRALAFSEINQQLYQLLNGLDVVYHAQGEYAYADVIVNSALEKLRKGSRQNLTAP
                     ATMIDWRPVVHEMRLFKSPEEIAVLRRAGEITAMAHTRAMEKCRPGMFEYHLEGEIHH
                     EFNRHGARYPSYNTIVGSGENGCILHYTENECEMRDGDLVLIDAGCEYKGYAGDITRT
                     FPVNGKFTQAQREIYDIVLESLETSLRLYRPGTSILEVTGEVVRIMVSGLVKLGILKG
                     DVDELIAQNAHRPFFMHGLSHWLGLDVHDVGVYGQDRSRILEPGMVLTVEPGLYIAPD
                     AEVPEQYRGIGIRIEDDIVITETGNENLTASVVKKPEEIEALMVAARKQ"
     gene            38382..39560
                     /gene="ubiH"
                     /locus_tag="PN977_000039"
                     /gene_synonym="visB"
     CDS             38382..39560
                     /gene="ubiH"
                     /locus_tag="PN977_000039"
                     /gene_synonym="visB"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-6-methoxyphenyl hydroxylase"
                     /protein_id="HDI6040127.1"
                     /translation="MSVIIVGGGMAGATLALAISRLSHGALPVHLIEATAPESHAHPG
                     FDGRAIALAAGTCQQLARIGVWQSLADCATAITTVHVSDRGHAGFVTLAAEDYQLAAL
                     GQVVELHNVGQRLFALLRKAPGVTLHCPDRVANVARTQSHVEVTLESGETLTGRVLVA
                     ADGTHSALATACGVDWQQEPYEQLAVIANVATSVAHEGRAFERFTQHGPLAMLPMSDG
                     RCSLVWCHPLERREEVLSWSDEKFCRELQSAFGWRLGKITHAGKRSAYPLALTHAARS
                     ITHRTVLVGNAAQTLHPIAGQGFNLGMRDVMSLAETLTQAQERGEDMGDYGVLCRYQQ
                     RRQSDREATIGVTDSLVHLFANRWAPLVVGRNIGLMTMELFTPARDVLAQRTLGWVAR
                     "
     gene            39583..40785
                     /gene="ubiI"
                     /locus_tag="PN977_000040"
                     /gene_synonym="visC"
     CDS             39583..40785
                     /gene="ubiI"
                     /locus_tag="PN977_000040"
                     /gene_synonym="visC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent 2-octaprenylphenol hydroxylase"
                     /protein_id="HDI6040128.1"
                     /translation="MQSVDVAIVGGGMVGLAVACGLQGSGLRVAVLEQRVQEPLAANA
                     PPQLRVSAINAASEKLLTRLGVWQDILSRRASCYHGMEVWDKDSFGHISFDDQSMGYS
                     HLGHIVENSVIHYALWNKAHQSSDITLLAPAELQQVAWGENETFLTLKDGSMLTARLV
                     IGADGANSWLRNKADIPLTFWDYQHHALVATIRTEEPHDAVARQVFHGEGILAFLPLS
                     DPHLCSIVWSLSPEEAQRMQQASEDEFNRALNIAFDNRLGLCKVESARQVFPLTGRYA
                     RQFASHRLALVGDAAHTIHPLAGQGVNLGFMDAAELIAELKRLHRQGKDIGQYIYLRR
                     YERSRKHSAALMLAGMQGFRDLFSGTNPAKKLLRDIGLKLADTLPGVKPQLIRQAMGL
                     NDLPEWLR"
     gene            complement(40859..40942)
                     /locus_tag="PN977_000041"
     CDS             complement(40859..40942)
                     /locus_tag="PN977_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6040129.1"
                     /translation="MSKNTKSKNNGIRKYNAKTEVKLVYFK"
     gene            41233..42327
                     /gene="gcvT"
                     /locus_tag="PN977_000042"
     CDS             41233..42327
                     /gene="gcvT"
                     /locus_tag="PN977_000042"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HDI6040130.1"
                     /translation="MAQQTPLYEQHTLCGARMVDFHGWMMPLHYGSQIDEHHAVRTDA
                     GMFDVSHMTIVDLRGSRTREFLRYLLANDVAKLTKSGKALYSGMLNASGGVIDDLIVY
                     YFTEDFFRLVVNSATREKDLSWITQHAEPFGIEITVRDDLSMIAVQGPNAQAKAATLF
                     NDAQRQAVEGMKPFFGVQAGDLFIATTGYTGEAGYEIALPNEKAADFWRALVEAGVKP
                     CGLGARDTLRLEAGMNLYGQEMDETISPLAANMGWTIAWEPADRDFIGREALEVQREH
                     GTEKLVGLVMTEKGVLRNELPVRFTDAQGNQHEGIITSGTFSPTLGYSIALARVPEGI
                     GETAIVQIRNREMPVKVTKPVFVRNGKAVA"
     gene            42351..42740
                     /gene="gcvH"
                     /locus_tag="PN977_000043"
     CDS             42351..42740
                     /gene="gcvH"
                     /locus_tag="PN977_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HDI6040131.1"
                     /translation="MSNVPAELKYSKEHEWLRKEADGTYTVGITEHAQELLGDMVFVD
                     LPEVGATVSAGDDCAVAESVKAASDIYAPVSGEIVAVNDALSDSPELVNSEPYAGGWI
                     FKIKASDESELESLLDATAYEALLEDE"
     gene            42859..45732
                     /gene="gcvP"
                     /locus_tag="PN977_000044"
     CDS             42859..45732
                     /gene="gcvP"
                     /locus_tag="PN977_000044"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HDI6040132.1"
                     /translation="MTQTLSQLENSGAFIERHIGPDAAQQQEMLNAVGAQSLNALTGQ
                     IVPKDIQLATPPQVGAPATEYAALAELKAIASRNKRFTSYIGMGYTAVQLPPVILRNM
                     LENPGWYTAYTPYQPEVSQGRLEALLNFQQVTLDLTGLDMASASLLDEATAAAEAMAM
                     AKRVSKLKNANRFFVASDVHPQTLDVVRTRAETFGFEVIVDDAQKVLDHQDVFGVLLQ
                     QVGTTGEIHDYTALISELKSRKIVVSVAADIMALVLLTAPGKQGADIVFGSAQRFGVP
                     MGYGGPHAAFFAAKDEYKRSMPGRIIGVSKDAAGNTALRMAMQTREQHIRREKANSNI
                     CTSQVLLANIASLYAVYHGPVGLKRIANRIHRLTDILAAGLQQKGLKLRHAHYFDTLC
                     VEVADKAGVLTRAEAAEINLRSDILNAVGITLDETTTRENVMQLFNVLLGDNHGLDID
                     TLDKDVAHDSRSIQPAMLRDDEILTHPVFNRYHSETEMMRYMHSLERKDLALNQAMIP
                     LGSCTMKLNAAAEMIPITWPEFAELHPFCPPEQAEGYQQMIAQLADWLVKLTGYDAVC
                     MQPNSGAQGEYAGLLAIRHYHESRNEGHRDICLIPASAHGTNPASAHMAGMQVVVVAC
                     DKNGNIDLTDLRAKAEQAGDNLSCIMVTYPSTHGVYEETIREVCEVVHQFGGQVYLDG
                     ANMNAQVGITSPGFIGADVSHLNLHKTFCIPHGGGGPGMGPIGVKAHLAPFVPGHSVV
                     QIEGMLTRQGAVSAAPFGSASILPISWMYIRMMGAEGLKKASQVAILNANYIASRLQD
                     AFPVLYTGRDGRVAHECILDIRPLKEETGISELDIAKRLIDYGFHAPTMSFPVAGTLM
                     VEPTESESKVELDRFIDAMLAIRAEIDQVKAGVWPLEDNPLVNAPHIQSELVAEWAHP
                     YSREVAVFPAGVADKYWPTVKRLDDVYGDRNLFCSCVPISEYQ"
     gene            45999..46742
                     /locus_tag="PN977_000045"
     CDS             45999..46742
                     /locus_tag="PN977_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDI6040133.1"
                     /translation="MAIALVTGGSRGIGRATALLLAQEGYTVAVNYQQNLHAAQEVMN
                     LITQAGGKAFVLQADISDENQVVAMFTAIDQHDEPLAALVNNAGILFTQCTVENLTAE
                     RINRVLSTNVTGYFLCCREAVKRMALKNGGSGGAIVNVSSVASRLGSPGEYVDYAASK
                     GAIDTLTTGLSLEVAAQGIRVNCVRPGFIYTEMHASGGEPGRVDRVKSNIPMQRGGQA
                     EEVAQAIVWLLSDKASYVTGSFIDLAGGK"
     gene            complement(46799..48232)
                     /gene="bglA"
                     /locus_tag="PN977_000046"
     CDS             complement(46799..48232)
                     /gene="bglA"
                     /locus_tag="PN977_000046"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase BglA"
                     /protein_id="HDI6040134.1"
                     /translation="MKKLTLPKDFLWGGAVAAHQVEGGWNKGGKGPSICDVLTGGAHG
                     VPREITKEVLPGKYYPNHEAVDFYGHYKEDIKLFAEMGFKCFRTSIAWTRIFPKGDEA
                     QPNEEGLKFYDDMFDELLKYNIEPVITLSHFEMPLHLVQQYGSWTNRKVVDFFVRFAE
                     VVFERYKHKVKYWMTFNEINNQRNWRAPLFGYCCSGVVYTEHENPEETMYQVLHHQFV
                     ASALAVKAARRINPEMKVGCMLAMVPLYPYSCNPDDVMFAQESMRERYVFTDVQLRGY
                     YPSYVLNEWERRGFNIKMEDGDLDVLREGTCDYLGFSYYMTNAVKAEGGTGDAISGFE
                     GSVPNPYVKASDWGWQIDPVGLRYALCELYERYQRPLFIVENGFGAYDKVEEDGSIND
                     DYRIDYLRAHIEEMKKAVTYDGVDLMGYTPWGCIDCVSFTTGQYSKRYGFIYVNKHDD
                     GTGDMSRSRKKSFNWYKEVIASNGEKL"
     gene            48277..48588
                     /locus_tag="PN977_000047"
     CDS             48277..48588
                     /locus_tag="PN977_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HDI6040135.1"
                     /translation="MQPNDITFFQRFQDDILAGRKTITIRDESESHFKTGDVLRVGRF
                     EDDGYFCTIEVTATSTVTLDTLTEKHAEQENMTLTELKKVIADIYPGQTQFYVIEFKC
                     L"
     gene            48752..49411
                     /locus_tag="PN977_000048"
     CDS             48752..49411
                     /locus_tag="PN977_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HDI6040136.1"
                     /translation="MVQKPLIKQGYSLAEEIANSVSHGIGLVFGIVGLVLLLVQAVDL
                     NASATAITSYSLYGGSMILLFLASTLYHAIPHQRAKMWLKKFDHCAIYLLIAGTYTPF
                     LLVGLDSPLARGLMIVIWNLALLGILFKLTIAHRFKILSLVTYLAMGWLSLVVIYEMA
                     VKLAAGSVTLLAVGGVVYSLGVIFYVCKRIPYNHAIWHGFVLGGSVCHFLAIYLYIGQ
                     A"
     gene            complement(49607..50587)
                     /gene="ygfZ"
                     /locus_tag="PN977_000049"
     CDS             complement(49607..50587)
                     /gene="ygfZ"
                     /locus_tag="PN977_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-modifying protein YgfZ"
                     /protein_id="HDI6040137.1"
                     /translation="MAFTPFPPRQPTASARLPLTLMTLDDWALATITGADSEKYMQGQ
                     VTADVSQMAEDQHLLAAHCDAKGKMWSNLRLFRDGDGFAWIERRSVREPQLTELKKYA
                     VFSKVTIAPDDERVLLGVAGFQARAALANLFSELPSKEKQVVKEGATTLLWFEHPAER
                     FLIVTDEATANMLTDKLRGEAELNNSQQWLALNIEAGFPVIDAANSGQFIPQATNLQA
                     LGGISFKKGCYTGQEMVARAKFRGANKRALWLLAGSASRLPEAGEDLELKMGENWRRT
                     GTVLAAVKLEDGQVVVQVVMNNDMEPDSIFRVRDDANTLHIEPLPYSLEE"
     gene            50830..51096
                     /gene="sdhE"
                     /locus_tag="PN977_000050"
     CDS             50830..51096
                     /gene="sdhE"
                     /locus_tag="PN977_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000354044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD assembly factor SdhE"
                     /protein_id="HDI6040138.1"
                     /translation="MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFI
                     RLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRNRERGPVAI"
     gene            51077..51484
                     /locus_tag="PN977_000051"
     CDS             51077..51484
                     /locus_tag="PN977_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin CptA"
                     /protein_id="HDI6040139.1"
                     /translation="MVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMV
                     LLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQEWSIVKAPWMIKSGMMLRLRSD
                     GGKRQHLWLAADSMDEAEWRDLRRILLQQETQR"
     gene            complement(51524..52045)
                     /gene="fldB"
                     /locus_tag="PN977_000052"
     CDS             complement(51524..52045)
                     /gene="fldB"
                     /locus_tag="PN977_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldB"
                     /protein_id="HDI6040140.1"
                     /translation="MNMGLFYGSSTCYTEMAAEKIRDIIGPELVTLHNLKDDSPKLME
                     QYDVLILGIPTWDFGEIQEDWEAVWDQLDDLNLEGKIVALYGLGDQLGYGEWFLDALG
                     MLHDKLSTKGVKFVGYWPTEGYEFTSPKPVIADGQLFVGLALDETNQYDLSDERIQSW
                     CEQILNEMAEHYA"
     gene            52157..53053
                     /gene="xerD"
                     /locus_tag="PN977_000053"
     CDS             52157..53053
                     /gene="xerD"
                     /locus_tag="PN977_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HDI6040141.1"
                     /translation="MKQDLARIEQFLDALWLEKNLAENTLNAYRRDLSMMVEWLHHRG
                     LTLATAQSDDLQALLAERLEGGYKATSSARLLSAVRRLFQYLYREKFREDDPSAHLAS
                     PKLPQRLPKDLSEAQVERLLQAPLIDQPLELRDKAMLEVLYATGLRVSELVGLTMSDI
                     SLRQGVVRVIGKGNKERLVPLGEEAVYWLETYLEHGRPWLLNGVSIDVLFPSQRAQQM
                     TRQTFWHRIKHYAVLAGIDSEKLSPHVLRHAFATHLLNHGADLRVVQMLLGHSDLSTT
                     QIYTHVATERLRQLHQQHHPRA"
     gene            53078..53788
                     /gene="dsbC"
                     /locus_tag="PN977_000054"
     CDS             53078..53788
                     /gene="dsbC"
                     /locus_tag="PN977_000054"
                     /EC_number="5.3.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional protein-disulfide
                     isomerase/oxidoreductase DsbC"
                     /protein_id="HDI6040142.1"
                     /translation="MKKGFMLFTLLAAFSGFAQADDAAIQQTLAKMGIKSSDIQPAPV
                     AGMKTVLTNSGVLYITDDGKHIIQGPMYDVSGTAPVNVTNKMLLKQLNALEKEMIVYK
                     APQEKHVITVFTDITCGYCHKLHEQMADYNALGITVRYLAFPRQGLDSDAEKEMKAIW
                     CAKDKNKAFDDVMAGKSVAPASCDVDIADHYALGVQLGVSGTPAVVLSNGTLVPGYQP
                     PKEMKEFLDEHQKMTSGK"
     gene            53794..55527
                     /gene="recJ"
                     /locus_tag="PN977_000055"
     CDS             53794..55527
                     /gene="recJ"
                     /locus_tag="PN977_000055"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HDI6040143.1"
                     /translation="MKQQIQLRRREVDETADLPAELPPLLRRLYASRGVRSAQELERS
                     VKGMLPWQQLSGVEKAVEILYNAFREGTRIIVVGDFDADGATSTALSVLAMRSLGCSN
                     IDYLVPNRFEDGYGLSPEVVDQAHARGAQLIVTVDNGISSHAGVEHARSLGIPVIVTD
                     HHLPGDTLPAAEAIINPNLRDCNFPSKSLAGVGVAFYLMLALRTFLRDQGWFDERNIA
                     IPNLAELLDLVALGTVADVVPLDANNRILTWQGMSRIRAGKCRPGIKALLEVANRDAQ
                     KLAASDLGFALGPRLNAAGRLDDMSVGVALLLCDNIGEARVLANELDALNQTRKEIEQ
                     GMQIEALTLCEKLERSRDTLPGGLAMYHPEWHQGVVGILASRIKERFHRPVIAFAPAG
                     DGTLKGSGRSIQGLHMRDALERLDTLYPGMMLKFGGHAMAAGLSLEEDKFKLFQQRFG
                     ELVTEWLDPSLLQGEVVSDGPLSPAEMTMEVAQLLRDAGPWGQMFPEPLFDGHFRLLQ
                     QRLVGERHLKVMVEPVGGGPLLDGIAFNVDTALWPDNGVREVQLAYKLDINEFRGNRS
                     LQIIIDNIWPI"
     gene            55618..56716
                     /gene="prfB"
                     /locus_tag="PN977_000056"
     CDS             join(55618..55692,55694..56716)
                     /gene="prfB"
                     /locus_tag="PN977_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311790.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HDI6040144.1"
                     /translation="MFEINPVNNRIQDLTERSDVLRGYLDYDAKKERLEEVNAELEQP
                     DVWNEPERAQALGKERSSLEAVVDTLDQMKQGLEDVSGLLELAVEADDEETFNEAVAE
                     LDALEEKLAQLEFRRMFSGEYDSADCYLDIQAGSGGTEAQDWASMLERMYLRWAESRG
                     FKTEIIEESEGEVAGIKSVTIKISGDYAYGWLRTETGVHRLVRKSPFDSGGRRHTSFS
                     SAFVYPEVDDDIDIEINPADLRIDVYRASGAGGQHVNRTESAVRITHIPTGIVTQCQN
                     DRSQHKNKDQAMKQMKAKLYELEMQKKNAEKQAMEDNKSDIGWGSQIRSYVLDDSRIK
                     DLRTGVETRNTQAVLDGSLDQFIEASLKAGL"
     gene            56726..58243
                     /gene="lysS"
                     /locus_tag="PN977_000057"
     CDS             56726..58243
                     /gene="lysS"
                     /locus_tag="PN977_000057"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HDI6040145.1"
                     /translation="MSEQHAQGADAVVDLNNELKTRREKLANLREQGIAFPNDFRRDH
                     TSDQLHAEFDGKENEELEALNIEVAVAGRMMTRRIMGKASFVTLQDVGGRIQLYVARD
                     DLPEGVYNEQFKKWDLGDILGAKGKLFKTKTGELSIHCTELRLLTKALRPLPDKFHGL
                     QDQEARYRQRYLDLISNDESRNTFKVRSQILSGIRQFMVNRGFMEVETPMMQVIPGGA
                     AARPFITHHNALDLDMYLRIAPELYLKRLVVGGFERVFEINRNFRNEGISVRHNPEFT
                     MMELYMAYADYKDLIELTESLFRTLAQDILGKTEVTYGDVTLDFGKPFEKLTMREAIK
                     KYRPETDMADLDNFDSAKAIAESIGIHVEKSWGLGRIVTEIFEEVAEAHLIQPTFITE
                     YPAEVSPLARRNDVNPEITDRFEFFIGGREIGNGFSELNDAEDQAQRFLDQVAAKDAG
                     DDEAMFYDEDYVTALEHGLPPTAGLGIGIDRMVMLFTNSHTIRDVILFPAMRPVK"
     gene            complement(58287..58835)
                     /gene="idi"
                     /locus_tag="PN977_000058"
     CDS             complement(58287..58835)
                     /gene="idi"
                     /locus_tag="PN977_000058"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HDI6040146.1"
                     /translation="MQTEHVILLNAQGVPTGTLEKYAAHTADTRLHLAFSSWLFNAKG
                     QLLVTRRALSKKAWPGVWTNSVCGHPQLGESNEDAVIRRCRYELGVEITPPESIYPDF
                     RYRATDPSGIVENEVCPVFAARTTSALQINDDEVMDYQWCDLADVLHGIDATPWAFSP
                     WMVMQATNREARKRLSAFTQLK"
     gene            58890..58961
                     /locus_tag="PN977_000059"
     CDS             58890..58961
                     /locus_tag="PN977_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6040147.1"
                     /translation="MINQVSVYRQPPVLSGCRQVKTI"
     gene            complement(58958..59083)
                     /locus_tag="PN977_000060"
     CDS             complement(58958..59083)
                     /locus_tag="PN977_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6040148.1"
                     /translation="MNFLMRAIFSLLLLFTLSIPVISDCVAMAIESRFKYMMLLF"
     gene            complement(59085..60533)
                     /gene="uacT"
                     /locus_tag="PN977_000061"
     CDS             complement(59085..60533)
                     /gene="uacT"
                     /locus_tag="PN977_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417364.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urate/proton symporter UacT"
                     /protein_id="HDI6040149.1"
                     /translation="MSAIDSQLPSSSGQDRPTDEVDRILSPGKLIILGLQHVLVMYAG
                     AVAVPLMIGDRLGLSKEAIAMLISSDLFCCGIVTLLQCIGIGRFMGIRLPVIMSVTFA
                     AVTPMIAIGMNPDIGLLGIFGATIAAGFITTLLAPLIGRLMPLFPPLVTGVVITSIGL
                     SIIQVGIDWAAGGKGNPQYGNPVYLGISFAVLIFILLITRYAKGFMSNVAVLLGIVFG
                     FLLSWMMNEVNLSGLHDASWFAIVTPMSFGMPIFDPVSILTMTAVLIIVFIESMGMFL
                     ALGEIVGRKLSSHDIIRGLRVDGVGTMIGGTFNSFPHTSFSQNVGLVSVTRVHSRWVC
                     ISSGIILILFGMVPKMAVLVASIPQFVLGGAGLVMFGMVLATGIRILSRCNYTTNRYN
                     LYIVAISLGVGMTPTLSHDFFSKLPAVLQPLLHSGIMLATLSAVVLNVFFNGYQHHAD
                     LVKESVSDKDLKVRTVRMWLLMRKLKKNEHGE"
     gene            60969..62888
                     /gene="ygfT"
                     /locus_tag="PN977_000062"
     CDS             60969..62888
                     /gene="ygfT"
                     /locus_tag="PN977_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent uric acid utilization protein
                     YgfT"
                     /protein_id="HDI6040150.1"
                     /translation="MNKFIAAEAAECIGCHACEIACAVAHNQENWPLSHSDFRPRIHV
                     VGKGQAANPVACHHCNNAPCVTACPVNALTFQSDSVQLDEQKCIGCKRCAIACPFGVV
                     EMVDTIAQKCDLCNQRSSGTQACIEVCPTQALRLMDDKGLQQIKVARQRKTAAGKASS
                     DAQPSRSAALLPVNSRKGADKISASERKTHFGEIYCGLDPQQATYESDRCVYCAEKAN
                     CNWHCPLHNAIPDYIRLVQEGKIIEAAELCHQTSSLPEICGRVCPQDRLCEGACTLKD
                     HSGAVSIGNLERYITDTALAMGWRPDVSKVVPRSEKVAVIGAGPAGLGCADILARAGV
                     QVDVFDRHPEIGGMLTFGIPPFKLDKTVLSQRREIFTAMGIDFHLNCEIGRDITFSDL
                     TSEYDAVFIGVGTYGMMRADLPHEDAPGVIQALPFLTAHTRQLMGLPESEEYPLTDVE
                     GKRVVVLGGGDTTMDCLRTSIRLNAASVTCAYRRDEVSMPGSRKEVVNAREEGVEFQF
                     NVQPQYIACDEDGRLTAVGLIRTAMGEPGPDGRRRPRPVAGSEFELPADVLIMAFGFQ
                     AHAMPWLQGSGIKLDKWGLIQTGDVGYLPTQTHLKKVFAGGDAVHGADLVVTAMAAGR
                     QAARDMLTLFDTKAS"
     gene            62888..63376
                     /locus_tag="PN977_000063"
     CDS             62888..63376
                     /locus_tag="PN977_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417362.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HDI6040151.1"
                     /translation="MKSLIIVNPADCIGCRTCEVACVVAHPSEQELNADVFLPRLKVQ
                     RLDSISAPVMCHQCENAPCVGACPVGALTMGEQVVQTNSARCIGCQSCVSACPFGMIT
                     IQSLPGDTRQQIVKCDLCEQREEGPACVESCPTQALQLLTERELRRVRQQRIVASGEN
                     PL"
     gene            complement(63412..64779)
                     /gene="ghxQ"
                     /locus_tag="PN977_000064"
                     /gene_synonym="ygfQ"
     CDS             complement(63412..64779)
                     /gene="ghxQ"
                     /locus_tag="PN977_000064"
                     /gene_synonym="ygfQ"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanine/hypoxanthine transporter GhxQ"
                     /protein_id="HDI6040152.1"
                     /translation="MSGDILQTPDAPKPQGALDNYFKITARGSTVRQEVLAGLTTFLA
                     MVYSVIVVPGMLGKAGFPPAAVFVATCLVAGFGSLLMGLWANLPMAIGCAISLTAFTA
                     FSLVLGQQISVPVALGAVFLMGVIFTAISVTGVRTWILRNLPMGIAHGTGIGIGLFLL
                     LIAANGVGMVIKNPIEGLPVALGAFTSFPVMMSLLGLAVIFGLEKCRVPGGILLVIIA
                     ISIIGLIFDPAVKYHGLVAMPSLTGEDGKSLIFSLDIMGALQPTVLPSVLALVMTAVF
                     DATGTIRAVAGQANLLDKDNQIINGGKALTSDSVSSIFSGLVGAAPAAVYIESAAGTA
                     AGGKTGLTATVVGALFLLILFLSPLSFLIPGYATAPALMYVGLLMLSNVSKLDFNDFI
                     DAMAGLVCAVFIVLTCNIVTGIMLGFVTLVVGRVFAREWQKLNIGTVIITAALVAFYA
                     GGWAI"
     gene            complement(64815..66131)
                     /gene="guaD"
                     /locus_tag="PN977_000065"
     CDS             complement(64815..66131)
                     /gene="guaD"
                     /locus_tag="PN977_000065"
                     /EC_number="3.5.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanine deaminase"
                     /protein_id="HDI6040153.1"
                     /translation="MSGEHTLKAVRGSFIDVTRTIDNPEEIASALRFIEDGLLLIKQG
                     KVEWFGEWENGKHQIPDTIRVRDYRGKLIVPGFVDTHIHYPQSEMVGAYGEQLLEWLN
                     KHTFPTERRYEDLEYAREMSAFFIKQLLRNGTTTALVFGTVHPQSVDALFEAASHINM
                     RMIAGKVMMDRNAPDYLLDTAESSYHQSKELIERWHKNGRLLYAITPRFAPTSSPEQM
                     AMAQRLKEEYPDTWVHTHLCENKDEIAWVKSLYPDHDGYLDVYHQYGLTGKNCVFAHC
                     VHLEEKEWDRLSETKSSIAFCPTSNLYLGSGLFNLKKAWQKKVKVGMGTDIGAGTTFN
                     MLQTLNEAYKVLQLQGYRLSAYEAFYLATLGGAKSLGLDDLIGNFLPGKEADFVVMEP
                     TATPLQQLRYDNSVSLVDKLFVMMTLGDDRSIYRTYVDGRLVYERN"
     gene            complement(66149..67549)
                     /gene="xanQ"
                     /locus_tag="PN977_000066"
     CDS             complement(66149..67549)
                     /gene="xanQ"
                     /locus_tag="PN977_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417358.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine/proton symporter XanQ"
                     /protein_id="HDI6040154.1"
                     /translation="MSDINHAGSDLIFELEDRPPFHQALVGAITHLLAIFVPMVTPAL
                     IVGAALQLSAETTAYLVSMAMIASGIGTWLQVNRYGIVGSGLLSIQSVNFSFVTVMIA
                     LGSSMKSDGFHEELIMSSLLGVSFVGAFLVVGSSFILPYLRRVITPTVSGIVVLMIGL
                     SLIKVGIIDFGGGFAAKSSGTFGNYEHLGVGLLVLIVVIGFNCCRSPLLRMGGIAIGL
                     CVGYIASLCLGMVDFSSMRNLPLITIPHPFKYGFSFSFHQFLVVGTIYLLSVLEAVGD
                     ITATAMVSRRPIQGEEYQSRLKGGVLADGLVSVIASAVGSLPLTTFAQNNGVIQMTGV
                     ASRYVGRTIAVMLVILGLFPMIGGFFTTIPSAVLGGAMTLMFSMIAIAGIRIIITNGL
                     KRRETLIVATSLGLGLGVSYDPEIFKILPASIYVLVENPICAGGLTAILLNIILPGGY
                     RQENVLPGITSAEEMD"
     gene            complement(67714..70584)
                     /locus_tag="PN977_000067"
     CDS             complement(67714..70584)
                     /locus_tag="PN977_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase
                     Mo/Fe-S-binding subunit"
                     /protein_id="HDI6040155.1"
                     /translation="MIIHFTLNGAPQELTVNPGENVQKLLFNMGMHSVRNSDDGFGFA
                     GSDAIIFNGNIVNASLLIAAQLEKADIRTAESLGKWNELSLVQQAMVDVGVVQSGYND
                     PAAALIITDLLDRIAAPTREEIDDALSGLFSRDAGWQQYYQVIELAVARKNNPQATID
                     IAPTFRDDLEVIGKHYPKTDAAKMVQAKPCYVEDRVTADACVIKMLRSPHAHALITHL
                     DVSKAEALPGVVHVITHLNCPDIYYTPGGQSAPEPSPLDRRMFGKKMRHVGDRVAAVV
                     AESEEIALEALKLIDVEYEVLKPVMSIDEAMAEDAPVVHDEPVVYVAGAPDTLEDDNS
                     HAAQRGEHMIINFPIGSRPRKNIAASIHGHIGDMDKGFADADVIIERTYNSTQAQQCP
                     TETHICFTRMDGDRLVIHASTQVPWHLRRQVARLVGMKQHKVHVIKERVGGGFGSKQD
                     ILLEEVCAWATCVTGRPVLFRYTREEEFIANTSRHVAKVTVKLGAKKDGRLTAVKMDF
                     RANTGPYGNHSLTVPCNGPALSLPLYPCDNVDFQVTTYYSNICPNGAYQGYGAPKGNF
                     AITMALAELAEQLQIDQLEIIERNRVHEGQELKILGAIGEGKAPTSVPSAASCALEEI
                     LRQGREMIQWSSPKPQNGDWHIGRGVAIIMQKSGIPDIDQANCMIKLESDGTFIVHSG
                     GADIGTGLDTVVTKLAAEVLHCPPQDVHVISGDTDHALFDKGAYASSGTCFSGNAARL
                     AAENLREKILFHGAQMLGEPVADVQLATPGVVRGKKGEVSFGDIAHKGETGTGFGSLV
                     GTGSYITPDFAFPYGANFAEVAVNTRTGEIRLDKFYALLDCGTPVNPELALGQIYGAT
                     LRAIGHSMSEEIIYDAEGHPLTRDLRSYGAPKIGDIPRDFRAVLVPSDDKVGPFGAKS
                     ISEIGVNGAAPAIATAIHDACGIWLREWHFTPEKILTALEKI"
     gene            complement(70581..71360)
                     /locus_tag="PN977_000068"
     CDS             complement(70581..71360)
                     /locus_tag="PN977_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase
                     FAD-binding subunit"
                     /protein_id="HDI6040156.1"
                     /translation="MIEQFFRPDSVEQALELKRRYQDEAVWFAGGSKLNATPTRTDKK
                     IAISLQDLELDWVDWDNGALRIGAMSRLQPLRDARFIPAALREALGFVYSRHVRNQST
                     IGGEIAARQEESVLLPVLLALDAELVFGNGETLSIEDYLACPCDRLLTEIIIKDPYRT
                     CATRKISRSQAGLTVVTAAVAMTDHDGMRIALDGVASKALRLHDVEKQNLEGNALEQA
                     VANAIFPQEDLRGSVAYKRYITGVLVADLYADCQQAGEEAV"
     gene            complement(71411..72739)
                     /gene="ssnA"
                     /locus_tag="PN977_000069"
     CDS             complement(71411..72739)
                     /gene="ssnA"
                     /locus_tag="PN977_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417355.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative aminohydrolase SsnA"
                     /protein_id="HDI6040157.1"
                     /translation="MLILKNVTAVQLHPAKVQEGVDIAIENDVIVAIGDALTQRYPDA
                     SFKEMHGRIVMPGIVCSHNHFYSGLSRGIMANIAPCPDFISTLKNLWWRLDRALDEES
                     LYYSGLICSLEAIKSGCTSVIDHHASPAYIGGSLSTLRDAFLKVGLRAMTCFETTDRN
                     NGIKELQEGVEENIRFARLIDEAKKATSEPYLVEAHIGAHAPFTVPDAGLEMLREAVK
                     ATGRGLHIHAAEDLYDVSYSHHWYGKDLLARLAQFDLIDSKTLVAHGLYLSKDDITLL
                     NQRDAFLVHNARSNMNNHVGYNHHLSDIRNLALGTDGIGSDMFEEMKFAFFKHRDAGG
                     PLWPDSFAKALTNGNELMSRNFGAKFGLLEAGYKADLTICDYNSPTPLLADNIAGHIA
                     FGMGSGSVHSVMVNGVMVYEDRQFNFDCDSIYAQARKAAASMWRRMDALA"
     gene            complement(72742..75840)
                     /gene="ygfK"
                     /locus_tag="PN977_000070"
     CDS             complement(72742..75840)
                     /gene="ygfK"
                     /locus_tag="PN977_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenate reductase subunit YgfK"
                     /protein_id="HDI6040158.1"
                     /translation="MGDIMRPIPFEELLTRIFDEYQQQRSIFGIPEQQFYSPVKGKTV
                     SVFGETCATPVGPAAGPHTQLAQNIVTSWLTGGRFIELKTVQILDRLELEKPCIDAED
                     ECFNTEWSTEFTLLKAWDEYLKAWFALHLLEAMFQPSDSGKSFIFNMSVGYNLEGIKQ
                     PPMQQFIDNMMDASDHPKFAQYRDTLNKLLQDDAFLARHGLQEKRESLQALPARIPTS
                     MVHGVTLSTMHGCPPHEIEAICRYMLEEKGLNTFVKLNPTLLGYARVREILDVCGFGY
                     IGLKEESFDHDLKLTQALEMLERLMALAKEKSLGFGVKLTNTLGTINNKGALPGEEMY
                     MSGRALFPLSINVAAVLSRAFDGKLPISYSGGASQLTIRDIFDTGIRPITMATDLLKP
                     GGYLRLSACMRELEGSDAWGLDHVDVERLNRLAADALTMEYTQKHWKPEERIEVAEDL
                     PLTDCYVAPCVTACAIKQDIPEYIRLLGEHRYADALELIYQRNALPAITGHICDHQCQ
                     YNCTRLDYDSALNIRELKKVALEKGWDEYKQRWHKPAGSGSRHPVAVIGAGPAGLAAG
                     YFLARAGHPVTLFEREANAGGVVKNIIPQFRIPAELIQHDIDFVAAHGVKFEYGCSPD
                     LTIEQLKNQGFHYVLIATGTDKNSGVKLAGDNQNVWKSLPFLREYNKGTALKLGKHVV
                     VVGAGNTAMDCARAALRVPGVEKATIVYRRSLQEMPAWREEYEEALHDGVEFRFLNNP
                     ERFDADGTLTLRVMSLGEPDEKGRRRPVETNETVTLLVDSLITAIGEQQDTEALNAMG
                     VPLDKNGWPDVDHNGETRLTDVFMIGDVQRGPSSIVAAVGTARRATDAILSRENIRSH
                     QNDKYWNNVNPAEIYQRKGDISITLVNSDDRDAFVAQEAARCLECNYVCSKCVDVCPN
                     RANVSIAVPGFQNRFQTLHLDAYCNECGNCAQFCPWNGKPYKDKITVFSLAQDFDNSS
                     NPGFLVEDCRVRVRLNNQSWVLNIDSKGQFNNVPPELNDMCRIISHVHQHHHYLLGRV
                     EV"
     gene            complement(76162..76740)
                     /gene="mocA"
                     /locus_tag="PN977_000071"
     CDS             complement(76162..76740)
                     /gene="mocA"
                     /locus_tag="PN977_000071"
                     /EC_number="2.7.7.76"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor cytidylyltransferase"
                     /protein_id="HDI6040159.1"
                     /translation="MSAIDCIITAAGLSSRMGQWKMMLPWEQGTILDTSIKNALQFCS
                     RIILVTGYRGNELHERYANQSNITIIHNPDYAQGLLTSVKAAVPAVQTEHCFLTHGDM
                     PTLTIDIFRKIWSLRNDGAILPLHNGIPGHPILVSKPCLMQAIQRPNVTNMRQALLMG
                     EHYSVEIENAEIILDIDTPDDFITAKERYTEI"
     gene            76843..77613
                     /gene="yqeC"
                     /locus_tag="PN977_000072"
     CDS             76843..77613
                     /gene="yqeC"
                     /locus_tag="PN977_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417352.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenium-dependent hydroxylase
                     accessory protein YqeC"
                     /protein_id="HDI6040160.1"
                     /translation="MKSIVDPSALVIDLGAQKRPTVISVVGAGGKTSLLFWLAELLQA
                     SGRRVLITTTTHMFMPTSHWPVVFCRDPAMLPHASLTSPISFCFHSWKANQGKVQGFT
                     PEAIDALVQRPECDVILIEADGSRGMPLKAPDEHEPCIPKSSCCVIAVMGGHTLGAKV
                     STENVHRWSQFADITGLTPDATLQLSDLVALVRHPQGAFKNVPQGCRRVWFINRFSQC
                     ENAIAQSELLQPLQQHDVEAIWLGDIQEHPAIARRFVN"
     gene            77661..79286
                     /locus_tag="PN977_000073"
     CDS             77661..79286
                     /locus_tag="PN977_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF2563 family selenium-dependent molybdenum
                     hydroxylase system protein"
                     /protein_id="HDI6040161.1"
                     /translation="MNIFTEAAKLEEQNCPFAMAQIVDSRGSTPRHSAQMLVRADGSI
                     VGTIGGGMVERKVIEESLQALQERKPRLFHGRMARNGADAVGSDCGGAMSVFISVHGM
                     RPRLVLIGAGHVNRAIAQSAALLGFDIAVADIYRESLNPELFPPSTTLLHAESFGAAV
                     EALDIRPDNFVLIATNNQDREALDKLIEQPIAWLGLLASRRKVQLFLRQLREKGVAEE
                     HIARLHAPVGYNIGAETPQEIAISVLAEILQVKNNAPGGLMMKPSHPSGHQLVVIRGA
                     GDIASGVALRLYHAGFKVIMLEVEKPTVIRCTVAFAQAVFDGEMTVEGVTARLATSSA
                     EAMKLTERGFIPVMVDPACSLLDELKPLCVVDAILAKQNLGTRADMAPVTIALGPGFT
                     AGKDCHAVIETNRGHWLGQVIYSGCAQENTGVPGNIMGHTTRRVIRAPAAGIMRSNVK
                     LGDLVKEGDVIAWIGEHEIKAPLTGMVRGLLNDGLAVVGGFKIGDIDPRGETADFTSV
                     SDKARAIGGGVLEALMMLMHQGVKATKEVLEVA"
     gene            complement(79507..80439)
                     /gene="arcC"
                     /locus_tag="PN977_000074"
     CDS             complement(79507..80439)
                     /gene="arcC"
                     /locus_tag="PN977_000074"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HDI6040162.1"
                     /translation="MSKKIVLALGGNALGDDLAGQMKAVKITSQAIVDLIAQGHEVIV
                     THGNGPQVGMINQAFEAAAKTEAHSPMLPMSVCVALSQGYIGYDLQNALREELLSRGI
                     NKPVATLVTQVEVDANDPAFLNPTKPIGSFFTEQEAEQLTKQGYTLKEDAGRGYRRVV
                     ASPKPVDIIEKETVKALVDAGQVVITVGGGGIPVIREGNHLRGASAVIDKDWASARLA
                     EMIDADMLIILTAVEKVAINFGKENEQWLDRLSLSDAERFIEEGHFAKGSMLPKVEAA
                     ASFARSRAGREALITVLSKAKEGIEGKTGTVICQ"
     gene            complement(80487..81872)
                     /gene="hyuA"
                     /locus_tag="PN977_000075"
     CDS             complement(80487..81872)
                     /gene="hyuA"
                     /locus_tag="PN977_000075"
                     /EC_number="3.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311773.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-phenylhydantoinase"
                     /protein_id="HDI6040163.1"
                     /translation="MRVLIKNGTVVNADGQAKQDLLIESGIVRQLGNNISPQLPYEEI
                     DATGCYVFPGGVDVHTHFNIDVGIARSCDDFFTGTRAAACGGTTTIIDHMGFGPNGCR
                     LRHQLEVYRGYAAHKAVIDYSFHGVIQHINHAILDEIPMMVEEGLSSFKLYLTYQYKL
                     NDDEVLQALRRLHESGALTTVHPENDAAIASKRAEFIAAGLTAPRYHALSRPLECEAE
                     AIARMINLAQIAGNAPLYIVHLSNGLGLDYLRLARANHQPVWVETCPQYLLLDERSYD
                     TEDGMKFILSPPLRNVREQDKLWCGISDGAIDVVATDHCTFSMAQRLQISKGDFSRCP
                     NGLPGVENRMQLLFSSGVMTGRITPERFVELTSAMPARLFGLWPQKGLLAPGSDGDVV
                     IIDPRQSQQIQHRHLHDNADYSPWEGFTCQGAIVRTLSRGETIFCDGTFTGKAGRGRF
                     LRRKPFVPPVL"
     gene            complement(81925..83136)
                     /locus_tag="PN977_000076"
     CDS             complement(81925..83136)
                     /locus_tag="PN977_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgeY family selenium metabolism-linked
                     hydrolase"
                     /protein_id="HDI6040164.1"
                     /translation="MAKNIPFKLILEKAKDYQADMTRFLRDMVAIPSESCDEKRVVHR
                     IKEEMEKVGFDKVEIDPMGNVLGYIGHGPRLVAMDAHIDTVGIGNIKNWDFDPYEGME
                     TDELIGGRGTSDQEGGMASMVYAGKIIKDLGLEDEYTLLVTGTVQEEDCDGLCWQYII
                     EQSGIRPEFVVSTEPTDCQVYRGQRGRMEIRIDVQGVSCHGSAPERGDNAIFKMGPIL
                     GELQELSQRLGYDEFLGKGTLTVSEIFFTSPSRCAVADSCAVSIDRRLTWGETWEGAL
                     DEIRALPAVQKANAVVSMYNYDRPSWTGLVYPTECYFPTWKVEEDHFTVKALVNAYEG
                     LFGKAPVVDKWTFSTNGVSIMGRHGIPVIGFGPGKEPEAHAPNEKTWKSHLVTCAAMY
                     AAIPLSWLATE"
     gene            complement(83194..84390)
                     /gene="dpaL"
                     /locus_tag="PN977_000077"
     CDS             complement(83194..84390)
                     /gene="dpaL"
                     /locus_tag="PN977_000077"
                     /EC_number="4.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopropionate ammonia-lyase"
                     /protein_id="HDI6040165.1"
                     /translation="MSVFSLKIDIADNKFFNGETSPLFSQSQAKLARQFHQKIAGYRP
                     TPLCALDDLANLFGVKKILVKDESKRFGLNAFKMLGGAYAIAQLLCEKYHLDIETLSF
                     EHLKNAIGEKMTFATTTDGNHGRGVAWAAQQLGQNAVIYMPKGSAQERVDAILNLGAE
                     CIVTDMNYDDTVRLTMQHAQQHGWEVVQDTAWEGYTKIPTWIMQGYATLADEAVEQMR
                     EMGVTPTHVLLQAGVGAMAGGVLGYLVDVYSPQNLHSIIVEPDKADCIYRSGVKGDIV
                     NVGGDMATIMAGLACGEPNPLGWEILRNCATQFISCQDSVAALGMRVLGNPYGNDPRI
                     ISGESGAVGLGVLAAVHYHPQRQSLMEKLALNKDAVVLVISTEGDTDVKHYREVVWEG
                     KHAVAP"
     gene            complement(84448..85635)
                     /gene="ygeW"
                     /locus_tag="PN977_000078"
     CDS             complement(84448..85635)
                     /gene="ygeW"
                     /locus_tag="PN977_000078"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="knotted carbamoyltransferase YgeW"
                     /protein_id="HDI6040166.1"
                     /translation="MKTVNELIKDINSLTSHLHEKDFLLTWEQTPDELKQVLDVAAAL
                     KALRAENISTKVFNSGLGISVFRDNSTRTRFSYASALNLLGLAQQDLDEGKSQIAHGE
                     TVRETANMISFCADAIGIRDDMYLGAGNAYMREVGAALDDGYKQGVLPQRPALVNLQC
                     DIDHPTQSMADLAWLREHFGSLENLKGKKIAMTWAYSPSYGKPLSVPQGIIGLMTRFG
                     MDVTLAHPEGYDLIPDVVEVAKNNAKASGGSFRQVTSMEEAFKDADIVYPKSWAPYKV
                     MEERTELLRANDHEGLKALEKQCLAQNAQHKDWHCTEEMMELTRDGEALYMHCLPADI
                     SGVSCKEGEVTEGVFEKYRIATYKEASWKPYIIAAMILSRKYAKPGALLEQLLKEAQE
                     RVK"
     gene            86114..87892
                     /locus_tag="PN977_000079"
     CDS             86114..87892
                     /locus_tag="PN977_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent transcriptional regulator"
                     /protein_id="HDI6040167.1"
                     /translation="MELATTQSVLMQIQPTIQRFARMLASVLQLEVEIVDENLCRVAG
                     TGAYGKFLGRQLSGNSRLLRHVLETKTEKVVTQSRFDPLCEGCDSKENCREKAFLGTP
                     VILQDRCVGVISLIAVTHEQQEHISDNLREFSDYVRHISTIFVSKLLEDQGPGDNISK
                     IFATMIDNMDQGVLVVDDENRVQFVNQTALKTLGVVQNNIIGKPIRFRPLTFESNFTH
                     GHMQHIVSWDDKSELIIGQLHNIQGRQLFLMAFHQSHTSFSVANAPDEPHIEQLVGEC
                     RVMRQLKRLISRIAPSPSSVMVVGESGTGKEVVARAIHKLSGRRNKPFIAINCAAIPE
                     QLLESELFGYVKGAFTGASANGKTGLIQAANTGTLFLDEIGDMPLMLQAKLLRAIEAR
                     EILPIGASSPIQVDIRIISATNQNLAQFIAEGKFREDLFYRLNVIPITLPPLRERQED
                     IELLVHYFLHLHTRRLGSVYPGIAPDVVEILRKHRWPGNLRELSNLMEYLVNVVPSGE
                     VIDSTLLPPNLLNNGTTEQSDVTEVSEAHLSLDDAGGTALEEMEKQMIREALSRHNSK
                     KQVADELGIGIATLYRKIKKYELLNT"
     gene            complement(87932..88411)
                     /gene="xdhC"
                     /locus_tag="PN977_000080"
     CDS             complement(87932..88411)
                     /gene="xdhC"
                     /locus_tag="PN977_000080"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase iron sulfur-binding
                     subunit XdhC"
                     /protein_id="HDI6040168.1"
                     /translation="MNHSETITIECTINGMPFQLHAAPGTPLSELLREQGLLSVKQGC
                     CVGECGACTVLVDGTAIDSCLYLAAWAEGKEIRTLEGEAKGGKLSHVQQAYAKSGAVQ
                     CGFCTPGLIMATTAMLAKPREKPLTITEIRRGLAGNLCRCTGYQMIVNTVLDCEKTK"
     gene            complement(88408..89286)
                     /gene="xdhB"
                     /locus_tag="PN977_000081"
     CDS             complement(88408..89286)
                     /gene="xdhB"
                     /locus_tag="PN977_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase FAD-binding subunit XdhB"
                     /protein_id="HDI6040169.1"
                     /translation="MFDFASYHRAATLADAINLLADNPQAKLLAGGTDVLIQLHHHND
                     RYRHIVDIHNLAELRGITLAEDGSLRIGSATTFTQLIEDPVTQRHLPALCAAASSIAG
                     PQIRNVATYGGNICNGATSADSATPTLIYDAKLEIHSPRGVRFVPINGFHTGPGKVSL
                     EHDEILVAFHFPPQPKEHAGSAHFKYAMRDAMDISTIGCAAHCRLDNGNFSELRLAFG
                     VAAPTPIRCQHAEQTAQNAPLNLQTLEAISESVLQDVAPRSSWRASKEFRLHLIQTMT
                     KKVISEAVAAAGGKLQ"
     gene            complement(89297..91594)
                     /gene="xdhA"
                     /locus_tag="PN977_000082"
     CDS             complement(89297..91594)
                     /gene="xdhA"
                     /locus_tag="PN977_000082"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase molybdenum-binding
                     subunit XdhA"
                     /protein_id="HDI6040170.1"
                     /translation="MEAREATATGESCMRVDAIAKVTGRARYTDDYVMAGMCYAKYVR
                     SPIAHGYAVSINDEQARSLPGVLAIFTWEDVPDIPFATAGHAWTLDENKRDTADRALL
                     TRHVRHHGDAVAIVVARDELTAEKAAQLVSIEWQELPVITTPEAALADDAEPIHNGGN
                     LLKQSTMSTGNVQQTIDAADYQVQGHYQTPVIQHCHMESVTSLAWMEDDSRITIVSST
                     QIPHIVRRVVGQALDIPWSCVRVIKPFVGGGFGNKQDVLEEPMAAFLTSKLGGIPVKV
                     SLSREECFLATRTRHAFTIDGQMGVNRDGTLKGYSLDVLSNTGAYASHGHSIASAGGN
                     KVAYLYPRCAYAYSSKTCYTNLPSAGAMRGYGAPQVVFAVESMLDDAATALGIDPVEI
                     RLRNAAREGDANPLTGKRIYSAGLPECLEKGRKIFEWEKRRAECQNQKGNLRRGVGVA
                     CFSYTSNTWPVGVEIAGARLLMNQDGTINVQSGATEIGQGADTVFSQMVAETVGVPVS
                     DVRVISTQDTDVTPFDPGAFASRQSYVAAPALRSAALLLKEKIIAHAAVMLHQSAMNL
                     TLIKGHIVLVERPEGPLMSLKDLAMDAFYHPERGGQLSAESSIKTTTNPPAFGCTFVD
                     LTVDIALCKVTINRILNVHDSGHILNPLLAEGQVHGGMGMGIGWALFEEMIIDAKSGV
                     VRNPNLLDYKMPTMPDLPQLESAFVEINEPQSAYGHKSLGEPPIIPVAAAIRNAVKMA
                     TGVAINTLPLTPKRLYEEFHLAGLI"
     gene            92009..92764
                     /locus_tag="PN977_000083"
     CDS             92009..92764
                     /locus_tag="PN977_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417341.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HDI6040171.1"
                     /translation="MSAGRLNKKSLGIVMLLSVGLLLAGCSGSKSSDTGTYSGSVYTV
                     KRGDTLYRISRTTGTSVKELARLNGISPPYTIEVGQKLKLGGAKSSSITRKSTAKSTT
                     KTASVTPSSAVPKSSWPPVGQRCWLWPTTGKVIMPYSTADGGNKGIDISAPRGTPIYA
                     AGAGKVVYVGNQLRGYGNLIMIKHSEDYITAYAHNDTMLVNNGQSVKAGQKIATMGST
                     DAASVRLHFQIRYRATAIDPLRYLPPQGSKPKC"
     gene            92843..92916
                     /locus_tag="PN977_000084"
     tRNA            92843..92916
                     /locus_tag="PN977_000084"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:92875..92877,aa:Gly,seq:ccc)
     gene            93150..94760
                     /locus_tag="PN977_000085"
     CDS             93150..94760
                     /locus_tag="PN977_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001613747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6040172.1"
                     /translation="MKGKSTLTLLLAGIFSCGTCQATGAEVTSESVFNILNSTGAATD
                     KSYLSLNPDKYPNYRLLIHSAKLKNEIKSHYTKDEIQGLLTLTENTRKLTLTEKPWGT
                     FILASTFEDDKTAAETHYDAVWLRDSLWGYMALVSDQGNSVAAKKVLLTLWDYMSTPD
                     QIKRMQDVISNPKRLDGIPGQMNAVHIRFDSNSPVMADVQEEGKPQLWNHKQNDALGL
                     YLDLLIQAIDTGTINAEDWQKGDRLKSVALLIAYLDKANFYIMEDSGAWEEDARLNTS
                     SVALVTSGLERLSNLLSKKDSVFVSDLLREAKANELDATFSTTRLNHLIDKGYERITL
                     QLDLGGESPGYLEKDKHYREADAALLNVIYPANLSKINTRRKEQVLKIVKKLAGPYGI
                     KRYEKDNYQSANFWFNDIKTDTDQNSHAKREMSFIPSTEAEWFFDSWYAKSAAIVYKE
                     SRKEEYLNDSVQFMNRSLAQITGENMIGANGRSVPEMALPESYNYIHKSGTLHEAPSP
                     IIPLNWSKASMTLMLKEMSNLINDEGNK"
     gene            94827..95006
                     /locus_tag="PN977_000086"
     CDS             94827..95006
                     /locus_tag="PN977_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GhoT/OrtT family toxin"
                     /protein_id="HDI6040173.1"
                     /translation="MINKLLLAYLIGLVVTSTLIFIFSEEKVTYRLFAAAITGLTWPL
                     SLIPSIISLMIRKSD"
     gene            95352..96101
                     /locus_tag="PN977_000087"
     CDS             95352..96101
                     /locus_tag="PN977_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDI6040174.1"
                     /translation="MIEEGLLLPMNVLLQGQKLTLLDPEMWFLRGNENKDVTLVITIG
                     NNHQKLMVVEDILLLIDRSQIEVTAGKVIYHPLRIDILSKLLAFIDESTGSVEREHHE
                     LFADALPFASEVVLFSKVASEAWFLATYLSSDNINEILFQHLRKTECYKLVRYLLSQS
                     LIQTSLYDLGELYGVSYSHFRRLCSYALGGKVKTELCGWRVARAVLEIIEGNSDMTTI
                     AHKYGYSSSSHFSAEVKSRLGKTPRELCKKL"
     gene            96098..97801
                     /locus_tag="PN977_000088"
     CDS             96098..97801
                     /locus_tag="PN977_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EscC/YscC/HrcC family type III secretion system
                     outer membrane ring protein"
                     /protein_id="HDI6040175.1"
                     /translation="MKIKLRITIILISALCIFNGLLTPGAYAATANGYVANKENLRSF
                     FETVSSYAGKPTIVSKLAMKKQISGNFDLTEPYALIERLSAQMGLIWYDDGKAIYIYD
                     SSEMRNALINLRKVSTNEFNNFLKKSGLYNSRYEIKGGGNGTFYVSGPPVYVDLVVNA
                     AKLMEQNSDGIEIGRNKVGIIHLVNTFVNDRTYELRGEKIVIPGMAKVLSTLLNNNIK
                     QSTGVNVLSEISSRQQLKNVSRMPPFPGAEEDDDLQVEKIISTAGAPETDNIQIIAYP
                     DTNSLLVKGTVSQVDFIEKLVATLDIPKRHIELSLWIIDIDKTDLEQLGADWSGTIKI
                     GSSLSASFNNSGSISTLDGTQFIATIQALAQKRRAAVVARPVVLTQENIPAIFDNNRT
                     FYTKLVGERTAELDEVTYGTMISVLPRFAARNQIELLLNIEDGNEINSDKTNVDDLPQ
                     VGRTLISTIARVPQGKSLLIGGYTRDTNTYESRKIPILGSIPFIGKLFGYEGTNANNI
                     VRVFLIEPREIDERMMNNANEAAVDARAITQQMAKNKEINDELLQKWIKTYLNREVVG
                     G"
     gene            97806..98951
                     /locus_tag="PN977_000089"
     CDS             97806..98951
                     /locus_tag="PN977_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YopN/LcrE/InvE/MxiC type III secretion system
                     gatekeeper"
                     /protein_id="HDI6040176.1"
                     /translation="MAIHVEHVGVLERAREVSRLEDIITEDNEDIEAEMPKMGDDPAG
                     KEARFLQATDEMSAALTQFMKKKIYEEQLANFLDGEEYVLEDQPIEKTDKVMEALKAA
                     TTHDYEVYSFAKKLFPDESDLVVVLRAILRKKQISENVRLNAEALLRKVNQETTKKFI
                     NSGINSALKAKLFGQALSLNPKLLRASYRQFLMAEDDAVDTYVEWIGSYGYQNRMLVT
                     KFIKETLFSDINALDASCSSLEFGMFLNKLSQLLSLQSAEALFLKTLMNNPIIKKFIS
                     AEDYWIFFLISLIKFPETAEELLKNALVTLPADANYKDKTLLLKAIYSGCTNLPFSLF
                     INNEQLLEIRECCKQAIKVTFAVELFDTQNSNKKQNKKPWKKVMFNV"
     gene            98944..101004
                     /locus_tag="PN977_000090"
     CDS             98944..101004
                     /locus_tag="PN977_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EscV/YscV/HrcV family type III secretion system
                     export apparatus protein"
                     /protein_id="HDI6040177.1"
                     /translation="MFNKVLIGLRSHPELIILGLMVMIIAMLIIPLPTYLIDFLIGLN
                     LTLAILVFLGSFYVDRILSFSSFPSILLITTLFRLALAISTSRLILLEADAGEIITSF
                     GEFVIGDSLVVGFVIFSIVTIVQFIVITKGSERVAEVAARFSLDGMPGKQMSIDADLR
                     AGIIDADLAKERRSVLERESQLYGSFDGAMKFIKGDAIANIIIIFVNIIGGLSVGVGQ
                     NGMDFSTALTVYTILTVGDGLVSQIPALLIAISAGFIVTRVNGDSDNMGQNIMSQLLS
                     NSFVIIVTCVLALSIGLLPGFPLLVFLCLAVILGIYFYFKFKKKGAEETVVEGDITVG
                     LEPYNQDDDISLGIINKLDQVITETVPLVLIMNSVQAKKYTEINLADRIRSQFFIEYG
                     IRIPGIVIREGEGLNDEDVILMLNEVRASQFKIYHDLVLLVEYSDEVVSTLIKKPVIV
                     NSNGEQYYWVTKSDAQKLTKIGCYTRTAMDEMYNHLSVCLAHNINEYFGIQETKYILD
                     QLEMKYPDLLKEILRYITVQRISEVIQRLIQERISVRNMRLVMEALALWSPREKDIIT
                     LVEHVRGALGRYICHKFSYSGEIKAIVISPEIEDRIRDGVRPTAGGTFLNLDASEAEM
                     ILDNFKLALSGINIPIKDIILLGSVDIRRFIKKLIESSYRDLEVLSYGELTENVPVNI
                     LKTI"
     gene            101036..102328
                     /gene="sctN"
                     /locus_tag="PN977_000091"
     CDS             101036..102328
                     /gene="sctN"
                     /locus_tag="PN977_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system ATPase SctN"
                     /protein_id="HDI6040178.1"
                     /translation="MKKLKLLNKYSYLHSINGSLIEAELDDVSVGEVCEIYASWQANE
                     RIARAQVVGFRNGKTLLNLIGSSVGLTRTAVLKPTGEQLTIQISDAFLGSVLNASGQI
                     MERFVPDTPGERGNLRLIDELPPSYQERRVINTPLETRIRVIDGVLTCGIGQRVGIFA
                     SAGCGKTVLMHMLVNNTEADVFVIGLIGERGREVTECAESLKKSVNAAKCVLVYATSD
                     FSSVDRCNAALMATTVAEYFRDRGKRVVLFIDSMTRYARALRDMKLAAGEPPARRGYP
                     ASVFDSLPRLLERPGPTLKGSITAFYTVLLEGEDESDPLGDEIRSILDGHIYLSRKLA
                     GQGHYPAIDVLKSVSRVFGQVTDEKHRDNAARVRKILTTLEDLQVFIDLGEYRAGQNA
                     ENDFAMNARPKLTNWLKQSVNEKMPMSETLKELERIVK"
     gene            102318..102776
                     /locus_tag="PN977_000092"
     CDS             102318..102776
                     /locus_tag="PN977_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion protein"
                     /protein_id="HDI6040179.1"
                     /translation="MLSKVNRLIRRTAQSLAACEASLQKLNAEKEKLAEKERLYDMQL
                     KNLQSLLDMKELLGEVVFRQDIFYSLRKVAVIQQQIAEINLEKQKIAERRKILNKEIV
                     QQQAQRKHWWLKGEKYDRLKKRIKKQLLNQMLYQDELEQEEKYNGRSQEN"
     gene            102754..103629
                     /locus_tag="PN977_000093"
     CDS             102754..103629
                     /locus_tag="PN977_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion protein"
                     /protein_id="HDI6040180.1"
                     /translation="MDEVKKIEHYKNLNTDTSENSAADLDSFLKKNKKNKEMSEIVAG
                     SILQLLRQAQDSQFPTFGKKNGQMKSIDNKSSLQVDRNSSFSLQTNGIEQLSTKKVLN
                     MTSRYQGVNSELSDKAIQFKGEIHNGIHTENITVQNHSNNKEREKKYRDGDKKNGEQA
                     HLLDITNERRFADNKIMFTQHIEKQRNVHTLNQNDINNSVNSANVPENELTYQFQRWG
                     QNHTVRILESSEGIRLKPSDTIVSDRLHEAQHNDVTAQRWVLTEQDERQGQRHQPHDE
                     QENEGKFENDQKDES"
     gene            103629..104576
                     /locus_tag="PN977_000094"
     CDS             103629..104576
                     /locus_tag="PN977_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001578916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YscQ/HrcQ family type III secretion apparatus
                     protein"
                     /protein_id="HDI6040181.1"
                     /translation="MFGLRKVNGNTHSFETTFLNWKEKGEDVALLMPEFSAKWLPIAE
                     ESGSWSGWVLLRELFPLISAELAGMALMPETERLIVEWLSLSSTPLNLKYPELKYNRL
                     CVGKVFDGVLSPAQPLIRIWTGELNVWLDKVTVCQYENAPTLDKKTLYWPIHFVIGFS
                     KSCYRTIVDIEVGDVLLISDNFAYAVIYNTKICDLIYPEELKMADHFEYEEDFETDDF
                     DIKKSEIEINDENDYQQINSFEDLPVKIEFVLGKKIMNLYEIDDLCAKRIIPLLPEAE
                     KNIEIRVNGALTGYGELVEVDDKLGVEIHSWLSGNNNVK"
     gene            104566..105231
                     /locus_tag="PN977_000095"
     CDS             104566..105231
                     /locus_tag="PN977_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EscR/YscR/HrcR family type III secretion system
                     export apparatus protein"
                     /protein_id="HDI6040182.1"
                     /translation="MSNSISLIAILSLFTLLPFIIASGTCFIKFSIVFVIVRNALGLQ
                     QVPSNMTLNGVALLLSMFVMMPVGKEIYNNSQNENLSFNNVASVVNFVETGMSGYKSY
                     LIKYSEPELVSFFEKIQKVNSSEDNEEIIDDDNISIFSLLPAYALSEIKSAFIIGFYI
                     YLPFVVVDLVISSVLLTLGMMMMSPVTISTPIKLILFVAMDGWTMLSKGLILQYFDLS
                     INP"
     gene            105241..105501
                     /locus_tag="PN977_000096"
     CDS             105241..105501
                     /locus_tag="PN977_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EscS/YscS/HrcS family type III secretion system
                     export apparatus protein"
                     /protein_id="HDI6040183.1"
                     /translation="MDDIVFAGNRALYLILVMSAGPIAVATFVGLLVGLFQTVTQLQE
                     QTLPFGVKLLCVSICFFLMSGWYGEKLYSFGIEMLNLAFARG"
     gene            105503..106270
                     /gene="sctT"
                     /locus_tag="PN977_000097"
     CDS             105503..106270
                     /gene="sctT"
                     /locus_tag="PN977_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000503299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     subunit SctT"
                     /protein_id="HDI6040184.1"
                     /translation="MGEAILYQLHSLLAATALGFCRLAPTFYLLPFFASGNIPTVVRH
                     PIIIVVSCALVQHYHYELSTLNEIDIALLAAREIIIGLFIACLLASPFWIFLAIGSFI
                     DNQRGATLSSTLDPATGVDTSELARLFNLFSAAVYLTNGGLNFILETLWQSYNLWPSG
                     NFNFPKLEPLFSYINNIMTHTIVYASPVIAVMLGGEAVLGLLARYASQLNAFAISLTV
                     KSALAFLILIIYFGPILAERVMPLSFFPEQLQLYIDK"
     gene            106279..107400
                     /locus_tag="PN977_000098"
     CDS             106279..107400
                     /locus_tag="PN977_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EscU/YscU/HrcU family type III secretion system
                     export apparatus switch protein"
                     /protein_id="HDI6040185.1"
                     /translation="MANKTEKPTQKKLQDASKKGQILKSRDLTVSVIMLVGTLYLGYV
                     FDVHHIMSILEYILDHNAKPDIWDYFKAMGIGWLKTIIPFLLVCMFTTILVSWFQSKM
                     QLATEAIKFKFDSLNPVNGLKRIFGLKTVKEFVKAILYIIFFALAIKVFWTNHKSLLF
                     KTLDGDIISLLSDWGEMLFLLILYCLGSMIIVLIFDFIAEYFLFMKDMKMDKQEVKRE
                     YKEQEGNPEIKSKRRERHQEILSEQLKSDVSNSRLMIANPTHIAIGIYFKPHLSPIPL
                     ISVRETNEVALAVRKYAKEIGIPIITDKKLARKIYATHRRYDYVSFENIDEILRLLLW
                     LEDVENAGQPVPDELLPSEDKFKEGEDTKSENKDNNLKN"
     gene            complement(107461..107754)
                     /locus_tag="PN977_000099"
     CDS             complement(107461..107754)
                     /locus_tag="PN977_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000060689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion protein"
                     /protein_id="HDI6040186.1"
                     /translation="MSLVRYNSKIIITVALISLLSACTRENSLKDTQNKRSGESQAIV
                     DARQFFSEHPEYISSAELEQKLYREFAQVTTVPAYKEMSMYQLLVISQDRLTH"
     gene            complement(107756..108256)
                     /locus_tag="PN977_000100"
     CDS             complement(107756..108256)
                     /locus_tag="PN977_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDI6040187.1"
                     /translation="MQVFSSDVYFTVGTNALLASQKEYYSDLVALVDLGHSFVVIDEH
                     QHRNLKPNTEPVNTLLSNNFIRINKNITLSDLTHFLVSNLHTQNVYSTQEPLTHDEID
                     ILRLCVSYSLKQIAIIKGIDYKTVSYHKIRALNKLNIKGTVELFIALCEWDKHYFKLQ
                     SCIRES"
     gene            108515..109693
                     /locus_tag="PN977_000101"
     CDS             108515..109693
                     /locus_tag="PN977_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001365813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrgH/EprH family type III secretion apparatus
                     protein"
                     /protein_id="HDI6040188.1"
                     /translation="MENNDKFLSQDLLESYAIRLLSGPLNGCEYEILNGRVLVIVGND
                     VSLGRSDSFSELPENTIVIPYGELTGSFEVIITTDPDLVVTIRELTAQEPEYRTLTLN
                     QQIEVLGLKFAVKGKNEVWQYSIPGIVENNITSTRQHFFSSTLFKYVTLFFLFAIIFI
                     AFYIVNASNDPQLRHIDKILVNKNRNYEILYGRDHVIYINTNSLDEAVWVKQALEKNQ
                     PGKPVRVINPDDESIRIFSWLADNFPDLQYFKLQLLDASNPRLTVSKQRNAITQQLID
                     NLIKGLLQTMPYASNISIAVLDDNVLESQAIETLSAIGLSYEKYKTANNVYFNIIGTL
                     SDSELNKINNYVDEYYKQWGKQYVRFNVNLKNQDTNNSSFSYGDNRFEKSQGSKWTFQ
                     "
     gene            109710..109949
                     /locus_tag="PN977_000102"
     CDS             109710..109949
                     /locus_tag="PN977_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system needle complex
                     protein"
                     /protein_id="HDI6040189.1"
                     /translation="MADWNGYIMDISKQFDQGVDDLNQQVEKALEDLATNPSDPKFLA
                     EYQSALAEYTLYRNAQSNVVKAYKDLDSAIIQNFR"
     gene            109969..110301
                     /gene="sctI"
                     /locus_tag="PN977_000103"
     CDS             109969..110301
                     /gene="sctI"
                     /locus_tag="PN977_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system inner rod subunit
                     SctI"
                     /protein_id="HDI6040190.1"
                     /translation="MSVSNMPPIDRAEQSAAHEIQQAKVIDLNDRVLNLDNPDDKMIS
                     AFANYAVQTENWQQNALQALRSDKKGLTPEKLLVLQDHVLNYNVEVSLVGTLARKIVA
                     AVETLTRS"
     gene            110298..111032
                     /locus_tag="PN977_000104"
     CDS             110298..111032
                     /locus_tag="PN977_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EscJ/YscJ/HrcJ family type III secretion inner
                     membrane ring protein"
                     /protein_id="HDI6040191.1"
                     /translation="MKYISLFLFILLLCGCKQQELLKHLDQQQANDVLAVLQRHNINA
                     EKKDQGKIGFSIYVEPTDFASAVDWLKIYNLPGKPDIQISQMFPADALVSSPRAEKAR
                     LYSAIEQRLEQSLKIMDGIVSSRVHVSYDVDTGDSGKTALPIHISVLAVYEKDINPEI
                     KINDIKRFIVNSFASVQYENISVVLSKRRDIIEQAPTYEINEPIFAYDKTMPVSILLA
                     LMSIATCWLLWKYRAILTNLIRLKNK"
     gene            111048..111629
                     /locus_tag="PN977_000105"
     CDS             111048..111629
                     /locus_tag="PN977_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion apparatus protein OrgA/MxiK"
                     /protein_id="HDI6040192.1"
                     /translation="MNLALRKIIYDPISYIHPQRVSWNNARISNPVLRSITNEMILLQ
                     YNLSVEHFSLNSSLIYYINNWKLFPLICLLSGCHFYRERFAERGFFYKVPDVLRNYLS
                     AIPVEINEKARYKLGIVNYQNIITCGFSTLLPYVRQQPLAMQQRFNLLFPDFVDHIQL
                     PLPVASTLLERITFYAKKNRDELDKLSCKWCCD"
     gene            111571..112278
                     /locus_tag="PN977_000106"
     CDS             111571..112278
                     /locus_tag="PN977_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6040193.1"
                     /translation="MRKKIEMSLINSPANGVVIKRIISDGLKEIVSLKEQILLETTAK
                     IQSIEEKREEKIIQGYYDGYAKGIIDVMDNFIPLISLLSSELEKNRVNMINDLKSILL
                     KSSEEVEVFIKIFESWVTKLPSISGPLNLYIPTSFKDKSIEVESYFVDKSIWNVHISY
                     HDDKRFIFFTDQFIAEFSPQEFVDNCEQYLISNHCFSPDKVNEICEHARHYLVERMCE
                     IDSLAMNNSDLTNPEDL"
     gene            112501..113133
                     /locus_tag="PN977_000107"
     CDS             112501..113133
                     /locus_tag="PN977_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDI6040194.1"
                     /translation="MGKIKIVVSDQQPFMIDGIIGFLGHYPDLYEVVGGYKDLKKAIA
                     ECNKSAAQIFILGEFAGGMMGSELIKWVKSHKIDAHIITFVAKMPYIDSIKLLEAGAK
                     GCVWKTSHPAKLNRAIDSISNGYTYFDSVHMDCEKISSRYSSDNQLTNRESEILQLIA
                     DGKTNKEIANFLQLSRKTVETHRLNIMKKLDVHSGIELIKTALRMGVCTI"
     gene            complement(113151..113654)
                     /locus_tag="PN977_000108"
     CDS             complement(113151..113654)
                     /locus_tag="PN977_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019843029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HDI6040195.1"
                     /translation="MRLPKVKILSIFMFSILITKMSFASSACFNEAGSIFRIEPNLIK
                     AIALVESNLKNDSIGKNRDKKNNIKSFDYGLMQINQMHIPMLKKRGIIKDERDLLDNP
                     CLNIKIGTEILYNHFSRCGVTWQCLGTYNAGFAMENQKKRLQYAKKIYVVYTRLNEID
                     KRKALAK"
     gene            complement(113738..113956)
                     /locus_tag="PN977_000109"
     CDS             complement(113738..113956)
                     /locus_tag="PN977_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6040196.1"
                     /translation="MTNPINMNNFSQSLNIATATGDEVVSLDKHITTSATDTDQIQAF
                     IVSTWMASFQNDMYSKDNPISPYYELEW"
     gene            complement(114124..115500)
                     /gene="ygeH"
                     /locus_tag="PN977_000110"
     CDS             complement(114124..115500)
                     /gene="ygeH"
                     /locus_tag="PN977_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HilA family transcriptional regulator YgeH"
                     /protein_id="HDI6040197.1"
                     /translation="MDLENKFSYHFLEGLTLTEDGILTQGNEQIYIPPKELGVLIVLL
                     ESAGHVVLKDMIIESVWKNIIVSDESLTRCIYSLRCIFEKIGYDRCIETIYRKGYRFS
                     GQVFKTKINEDNTSDYSIAIFPFTTSLNTLDPLILNQELVQNISDKKIDGLYTYPMAA
                     TNFCNDHISQNSFLRRFKPDYFVTGRINQNNAVKTLYIELIDAKNLFLIASNHLPVDE
                     LHNTSQFIIDNILQTVHNPERSVRLAKPNQGYKNHYLSDELLAGKKELYEFTPESIYR
                     AMAIFDGLQNKSDIQTLKIECYCLLAECHMSLALHGKYELELAAQKAFELLHYVSDIT
                     NVDGKILAIMGLITGLSGQAKVSHILFEQAKIHTTDIASLYYYRALVNFHNERIEEAR
                     ICIDKSLQLEPRRRKAVVIKECVDMYVPNPLKSNMKLYYKETSSESHRVIIDNILKLK
                     QLTRICMR"
     gene            complement(115835..116326)
                     /locus_tag="PN977_000111"
     CDS             complement(115835..116326)
                     /locus_tag="PN977_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HDI6040198.1"
                     /translation="MSTDTLEIFNNSDEWASQLKHALSQGENLALLHGLTPDILDRIY
                     ACAFDYHEKGNITDAEIYYKFLCIYAFENHEYLKGFASVCQSKKKYQQAYDLYKLSYN
                     YSPYDDYSVIYRMGQCQIGAKNIDNAMQCFYHIINNCEDESVKSKAQAYIELLTDNSE
                     DNG"
     gene            complement(116552..117043)
                     /locus_tag="PN977_000112"
     CDS             complement(116552..117043)
                     /locus_tag="PN977_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6040199.1"
                     /translation="MKPRNINNSLPLQPLVPDQENKNKKNEEKYVNPVKITMRSGLNY
                     IEQESLGGKYLTHDLSIKITDISEEIIQQAILSAMSIYKFPITDDLMSMATNELIKLA
                     KIENNLDLNKFSTICTDVLSPLVTRHNKEKKPDDLLPFAKIPFLIFIEKMEIDNMNPG
                     HAH"
     gene            complement(117612..118840)
                     /locus_tag="PN977_000113"
     CDS             complement(join(117612..118525,118525..118840))
                     /locus_tag="PN977_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558215.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HDI6040200.1"
                     /translation="MIDVLGPEKRRRRTTQEKIAIVQQSFEPGMTVSLVARQHGVAAS
                     QLFLWRKQYQEGSLTAVAAGEQVVPASELAAAMKQIKELQRLLGKKTMENELLKEAVE
                     YGRGKKVDSARALIARGWGVSLVSRCLRVSRAQLHVILRRTDDWMDGRRSRHTDDTDV
                     LLRIHHVIGELPTYGYRRVWALLRRQAELDGMPAINAKRVYRIMRQNALLLERKPAVP
                     PSKRAHTGRVAVKESNQRWCSDGFEFRCDNGEKLRVTFALDCCDREALHWAVTTGGFN
                     SETVQDVMLGAVERRFGNELPASPVEWLTDNGSCYRANETRQFARMLGLEPKNTAVRS
                     PESNGIAESFVKTIKRDYISVMPKPDGLTAAKNLAEAFEHYNEWHPHSALGYRSPREY
                     LRQQASNGLSDNRCLEI"
BASE COUNT        30722 a        27606 c        29828 g        30917 t
ORIGIN      
        1 tcacgatgcc gagcttgatc attattcttc tccgttagcc caaatcgcca aatcgtactt
       61 gtagcgcggt aatggcggtg agcgcagttg tctctgtacg caaaacgcga ggtcccaaca
      121 ggatatcagt aaattgatag cgggcagtca tggcaatttc atctgccgat aaaccgcctt
      181 ccgggccaat cagcaggcgg acgcgttcaa ccggtaacgg caacgtattg atgctgttac
      241 tggcgcgcgg gtgaagattc agtttcagtc cttcatcctg ctctgcacac caggcttcca
      301 gatccatcgc tggacggatt tccggcaccc ggttacgacc acactgctca caggcagcaa
      361 ttgcaatctt ctgccactgc tgaagcttct tgttcagacg ttcactatcc agtttaacgc
      421 cgcagcgctc agaaaaaagt ggcgtaatga ggcttacacc gagttcgatc gatttctgga
      481 tagtaaattc cattttttca ccacgcgaca tcacctgacc gaggtgaata tgcagcggag
      541 attcgcgatc gtcgatctgg ccttccagca ccttcacttc cacgcttttt ttgctggcgc
      601 tggtaatttc ggcgtcaaag acctggttgc taccgtcaaa caattgcaac gcctgccccg
      661 gccccatgcg cagtacgcgc ccgatatggt tggcggcatc ttcgcaaagc gcgatgtgag
      721 aatggctggt cagtggttca ggatgataaa tgcgggggat acgcatagtt aaaaatccgc
      781 gtcgtctccc cacgcggttg tacgcgtggg gtaggggtta acaaaaagaa tcccgctagt
      841 gtaggttagc tctttcgcgc ctggcaagcg cgttgcacat acgggttatg attgccctgc
      901 accttcgcga tgcgttcatc gcgctcgcac tcccagtcgg taaccggata catcttgttc
      961 catgcgttga acagctgcgt ttgctggcga gagagtgtca ggttgtattg gtcgcgcata
     1021 tagaagtagg tgcgcgcaat ggcaccgcgt gcacgcgctg gtggttcggc agctttttct
     1081 ttgaaatcga ccttcatggc gcattgaccg tactggcctt caccgccatt ccactggctg
     1141 tacataaagt tgccgcgatc gccattcacc tcaccgactg acggctgcag gttatgcata
     1201 tcgctttcca tcttgcgata gaccggatct ttagcgcagt ttttacgtcc accgtcctgc
     1261 cagcactggc gctggtgacc gaactgccag gcgggaacga catgttccca ctctacgcgg
     1321 ctggcgcggt tttcattttt gcgcacctga tagccgcacg attgcagatc aacaacgcct
     1381 tttttgccct gccagttaat tttacatccg caataaaacg taccgggcgc gtcagcgtgg
     1441 acttttaccg ccgcggcttt cgcctgagaa aaactattga taccttcggc caacgccggg
     1501 ccggaaaatg ctgcgctcag taccaccgca gcaatagaca aataacggta catcacttac
     1561 tccgtgttaa aacgagccag caacgtagcg aaagctgttt tggtatgcaa tcagccagat
     1621 caggaaagtt cctgatagtt cagatggtta tttcgcaacc agctgttcac cgcagtgaac
     1681 acagcgatag acggcctcgc cacgaactac gcgattatgg cggcgtacgg taagctgatg
     1741 ctcctggcac ttgcagcggt aggggaaggt gttgcgacgc acggattgca gttcgaactg
     1801 atgcgtacga cgggcgggaa cacccagcac gttttccatc atccacttcc actctttgcc
     1861 atgtggcgct acgcggccga aatgtttcca taccagcaaa tgtgccagtt cgtgcggtac
     1921 cacttcttca ataaaagctt cactgttttc caacagcaaa acgggattga ggcgaatttc
     1981 atagctttcc agccaggccg ttccggcgga ggttccgcgc tgggtgtaag agagttttgg
     2041 ctccgggtag ttacgcccta gcttcaggtt ggcctgggcg agtttttccc gcaggcgacg
     2101 cataacggcc tgttggatgg cgatagggag acgggatgtt ttcatagcgg cagaggatag
     2161 agcgaagaaa agatgactgc aagaggtggc ttcctccgcg atgggaggaa gcttggggag
     2221 attaatcgtg agcgcctatt tcgcgcagtt tacgaccttt catcagatta cgttcaatat
     2281 gttccagcga aacgtgtttg gtttccggta ccagccacaa tgtcagcagg ataaacagta
     2341 cgttcagagc cgcatacacc cagaaggtgt tggcgttacc cagcgtgttg agcatggtca
     2401 ggaacgttgc gccaacgatc atgttggcaa tccagttggt ggcagtggag caggtgatgc
     2461 caaaatcgcg gcctttcagc ggctgaattt cggagcacag tacccaaatc agcggaccgg
     2521 cactcatggc aaaaccgaca ataaacatca gcagcatggc gatggcgaaa tactgcgccg
     2581 acggagagtg aataccgata tgcatcattg taccgagtac gcccatgcca gcagccatca
     2641 ccaggaagcc cagcgttagc gttggtttac gtccccagcg gtcaacaagg ccgattgcga
     2701 taaaggtggc aagtacgttg gtcaggccga caatcacggt cccccacatt tgctcggtag
     2761 tgttggtata acccgccagt tcgaagattt tcggcgcgta atacatgatg acgttcatcc
     2821 cggtgaattg ctgcattacc tgcaacagta cgccaaggaa caccgcgcgg cggaagttgc
     2881 tgttctcttt aaacagcgcc cagccactct gtttaacctg caaactttca cggatttcat
     2941 ccagttcgcg tttcgcttcc gcgctggtgt cacgcaggcg tagcagcacg cgttcggcat
     3001 caacaaaacg gcgtttggcg gcaaaccaac gtgggctgtc tggcaggaag aagacaccaa
     3061 tcagcagcaa aattgccggg atgataatca cacccagcat ccagcgccat gcaccggtgt
     3121 agctgaaggc ggtatcagaa agataagcac cgaggatccc gatagtgatc atcaactgat
     3181 acatcgagat catactgcca cgaatttttt ccggcgcaat ttcagagagg tacagcggtg
     3241 cggtataaga ggccacaccc accgccagcc ccagtagaac gcgggaaaga atcagtactt
     3301 caacgtttgg cgcagccgca gagaacagcg aaccggcaac aaacaaaatt gcgccgatca
     3361 tcaggctctt tttgcgcccg agtttaaagg agagccagcc gctgcccacc gcaccgactg
     3421 ccgcaccgaa catcatggag cttacgaccc attcttgcgt gtgcgaagta atctggaatt
     3481 catctgcaat aaacggcagt gcgccagcaa ttacaccgat atccaggcca aagagtaatc
     3541 ccgccagagc ggcaaggaag cagacgaaaa acgtcattgc cttgtttgac cgcccctgtt
     3601 ttttagcgtc aggcatgatg ccctccaata tggttatttt ttattgtgaa ttaagatagg
     3661 tgagtacgac gtaaaaagat gtgaagcaaa tcacatcagt gtaatcgctt acactaacat
     3721 ttatcttagt tatgatttaa gtgcatgaaa ataattgagt tatatcatca tagattaaac
     3781 gctgttatct gcaattaaga ctttactgaa aagaaatgta acaactgtga aaaccgaata
     3841 ataatatgaa aatattacaa atctttgtct gttgtggttg gtgtaatcgg tttcagttgt
     3901 gatgataatc tgcggtgaaa cgtgatcagg aagaatgatg ttattgtgcg ggcggttttt
     3961 ttgccggatg cggcgtgaac gcctatccaa aagaaaaagg ccagcgcgag gctggccttt
     4021 gaacgcaggt gaagaaagat tacttcagac cggcagcatc gcgcagcagc tgcgctttgt
     4081 cggttttttc ccacgggaaa tgttcacgac caaagtgacc gtatgctgcg gtttctttgt
     4141 agatcgggtg cagcagatcc agcatctgaa tcagaccgta tgggcgcagg tcgaagaact
     4201 cacgtaccag cagggtcagt tgttcagaag gcactttctc agtaccgaaa gtttctacca
     4261 tgatggaggt cggttcagcc acgccgattg cgtaggaaac ctgaatttca caacgatcgg
     4321 ccaggccagc agcaacgatg tttttcgcga cataacgtgc tgcgtaggct gcggaacggt
     4381 ccacttttga tggatcttta ccagagaatg caccgccacc gtgacgcgcc atgccgccgt
     4441 aggtatcaac gataatttta cgaccagtca gaccgcagtc acccattggg ccaccgataa
     4501 cgaaacgacc ggtcgggttg atgaagaatt tggtggcaga agtcagccat tcagcgggca
     4561 gaattggctt gatgatctct tccattaccg cttcttgcag cgatttctgg tcgatctctt
     4621 cagagtgctg agtggaaagc acgacagcat cgataccaac gattttgccg tcgtcatact
     4681 gaaaagtcac ctggcttttc gcgtccgggc gcagccacgg cagagtgccg tttttacgca
     4741 cttcagcctg acgctgtacc agacggtgtg cataggtgat aggtgctggc atcagcacgt
     4801 cggtttcatt agttgcgtag ccaaacatca gaccctggtc acccgcgccc tgttccagcg
     4861 gatcggcacg gtcaacgccc tggttgatgt caggagactg tttgccgata gcgctcagaa
     4921 ccgcacagga gttagcgtca aagcccatgt cggaatgcac atagccaatt tcgcgaacgg
     4981 tgttacgggt gatctcttcg atgtctaccc aggcgctggt ggtgatttcg ccgccaacta
     5041 aaaccatgcc ggtttttacg taggtttcgc aagcaacgcg tgctttcgga tcctgttcga
     5101 ggatcgcgtc taaaacggca tcagaaattt ggtcagcaat tttgtcagga tgcccttcag
     5161 agacggactc ggacgtaaaa aggtgttttg ccatatttaa tatcacctaa agagaatttg
     5221 gttagctcaa actgttgtgt ggattttctg tggtagcgga tcctaccacg actctgcagg
     5281 ttaaaaacac tggcagtctg agtgttaatc ggtatggatg gattaacatc tggatggcta
     5341 ttttaggtca attcttcacc ctatttccac ttttttttga atcgtgtctc attctgttaa
     5401 aaacgtggct ggaaattttt cctgacaatg ccggcattct gcgtatttat cttttgcaat
     5461 tttctgccat tgtggggtat aaaacgcggc gcgcggctta aataaaaagc acacgacgtt
     5521 tctttcgtgt tgccacttcc agccgggttg aaatcagagt tttggcttgt gggttcgtct
     5581 taacaggcgg ccgtggaggt gatacgaaat aatgaaccgt tgtctgctgc ttaacctgtc
     5641 tcaccgttct ggtgaagatt cgttccccgc actctgcatc tctgctttgc atacctgccg
     5701 atgttatacc catctcggcg cttctcagga ttcaagagct ggttacagtt actgaggact
     5761 gaacaagggc gctcttgtaa aaacaagagt tttctcgtgg tttcgccgaa ctttcacact
     5821 tacgttcggt tatgtgctta ataatgttat gaaaaagaaa ccggttgcgc agttggagcg
     5881 tcagcattca ctgctggaaa atccatgtgc ttatgggttg ttatcgcagt tccaggctgc
     5941 gatagtcgtt aactgtttta cacttaataa aataatttga ggttcgctat gtctgacgac
     6001 atgtctatgg gtttgccttc gtcagcgggc gaacacggtg tactacgctc catgcaggag
     6061 gttgcaatga gctcccagga agccagcaag atgctgcgta cttacaatat tgcctggtgg
     6121 ggcaataact actatgacgt taacgagctg ggccacatta gcgtgtgccc ggacccggac
     6181 gtcccggaag ctcgcgtcga tctcgcgcag ttagtgaaaa ctcgtgaagc acagggccag
     6241 cgtctgcctg cactgttctg tttcccacag atcctgcagc accgtttgcg ttccattaac
     6301 gccgcgttca aacgtgcgag ggaatcctac ggctataacg gcgattactt ccttgtttat
     6361 ccgatcaaag ttaaccagca ccgccgcgtg attgagtccc tgattcattc gggcgaaccg
     6421 ctgggtctgg aagccggttc caaagccgag ttgatggcag tactggcaca tgctggcatg
     6481 acccgtagcg tcatcgtctg caacggttat aaagaccgcg aatatatccg cctggcatta
     6541 attggcgaga agatggggca caaggtctat ctggtcattg agaagatgtc agaaatcgcc
     6601 attgtgctgg atgaagcaga acgtctgaat gtcgttcctc gtctgggcgt gcgtgcacgt
     6661 ctggcttcgc agggttcggg taaatggcag tcctccggcg gggaaaaatc gaagttcggc
     6721 ctggctgcga ctcaggtact gcaactggtt gaaaccctgc gtgaagccgg gcgtctcgac
     6781 agcctgcaac tactgcactt ccacctcggt tcgcagatgg cgaatattcg cgatatcgcg
     6841 acaggcgttc gtgaatccgc gcgtttctat gtggaactgc acaagctggg cgtcaatatt
     6901 cagtgcttcg acgtcggcgg cggtctgggc gtggattatg aaggtactcg ttcgcagtcc
     6961 gactgttcgg tgaactacgg cctcaatgaa tacgccaaca acattatctg ggcgattggc
     7021 gatgcgtgtg aagaaaacgg tctgccgcat ccgacggtaa tcaccgaatc gggtcgtgcg
     7081 gtgactgcgc atcacaccgt gctggtgtct aatatcatcg gcgtggaacg taacgaatac
     7141 acggtgccga ccgcgcctgc agaagatgcg ccgcgcgcgc tgcaaagcat gtgggaaacc
     7201 tggcaggaga tgcacgaacc gggaactcgc cgttctctgc gtgaatggtt acacgacagt
     7261 cagatggatc tgcacgacat tcatatcggc tactcttccg gcatctttag cctgcaagaa
     7321 cgtgcatggg ctgagcagct ttatttgagc atgtgccatg aagtgcaaaa gcagctggat
     7381 ccgcaaaacc gtgctcatcg tccgattatc gacgagctgc aggaacgtat ggcggacaaa
     7441 atgtacgtca acttctcgct gttccagtcg atgccggacg catgggggat cgaccagttg
     7501 ttcccggttc tgccgctgga agggctggat caagtgccgg aacgtcgcgc tgtgctgctg
     7561 gatattacct gtgactctga cggtgctatc gaccactata ttgatggtga cggtattgcc
     7621 acgacaatgc caatgccgga gtacgatcca gagaatccgc cgatgctcgg tttctttatg
     7681 gtcggcgcat atcaggagat cctcggcaac atgcacaacc tgttcggtga taccgaagcg
     7741 gttgacgtgt tcgtcttccc tgacggtagc gtagaagtag aactgtctga cgaaggcgat
     7801 accgtggcgg acatgctgca atatgtacag ctcgatccga aaacgctgtt aacccagttc
     7861 cgcgatcaag tgaagaaaac cgatcttgat gctgaactgc aacaacagtt ccttgaagag
     7921 ttcgaggcag gtttgtacgg ttatacttat cttgaagatg agtaagtcct gtgttacttg
     7981 aatccgctta atttagcggt gataatccgc cacaatttat tgtgacaaat ccaacccttc
     8041 ctcgtcgggc ctaacgacgc ggaagggttt ttttatatcg actttgtaat aggagtccat
     8101 ccatgagcac cttaggtcat caatacgata actcactggt ttccaatgcc tttggttttt
     8161 tacgcctgcc gatgaacttc cagccgtatg acagcgatgc agactgggtg attactggcg
     8221 tgccgttcga tatggccact tctggtcgtg cgggtggtcg ccacggtccg gcagcgatcc
     8281 gtcaggtttc gacgaatctg gcctgggaac acaaccgctt cccgtggaat ttcgacatgc
     8341 gtgagcgtct gaacgtcgtg gactgcggcg atctggtata tgcctttggc gatgcccgtg
     8401 agatgagcga aaagctgcag gcgcacgccg agaagctgct ggctgccggt aagcgtatgc
     8461 tctctttcgg tggtgaccac tttgttacgc tgccgctgct gcgtgctcat gcgaagcatt
     8521 tcggcaaaat ggcgctggta cactttgacg cccacaccga tacctatgcg aacggttgtg
     8581 aatttgacca cggcactatg ttctataccg cgccgaaaga aggtctgatc gacccgaatc
     8641 attccgtgca gattggtatt cgtaccgagt ttgataaaga caacggcttt accgtgctgg
     8701 acgcctgcca ggtgaacgat cgcagcgtgg atgacgttat cgcccaagtg aaacagattg
     8761 tgggtgatat gccggtttac ctgacttttg atatcgactg cctggatcct gcttttgcac
     8821 caggcaccgg tacgccagtg attggcggcc tgacctccga tcgcgctatt aaactggtac
     8881 gcggcctgaa agatctcaac attgttggga tggacgtagt ggaagtggct ccggcatacg
     8941 atcagtcgga aatcactgct ctggcagcgg caacgctggc gctggaaatg ctgtatattc
     9001 aggcggcgaa aaagggcgag taagcaccag atgcgatgcg cacgggtaaa acgtgccatt
     9061 aatgtcggat gcggcgtgaa cgccttatcc gacctacgtt cggcacccgt aggccggata
     9121 agatgcgcca gcatcgcatc cggcaatgcg cacaaggtaa caaatgtgcc attcatgtca
     9181 gatgcggcgt gaacgcctta tctgacctac gttcgacacc accaggcttt acttaatccc
     9241 atccgcgctc atgcgatcgc gaatatgctg ggcgcgttcg gcggatgcag gatggtcgtc
     9301 aaacattgag ctttggcgac cttcttccag ttttgccagt ttttcaaagc tggtggcaag
     9361 acctgccggg ctgatgccgc gttggcgcag aagatcgtaa gaataatcat cggcttctgc
     9421 ttcctggcgc tgggagaatt gcgaattgac taatttctcg cccagattac caagttgtga
     9481 ttgagataaa cttccgacaa tcccgcccgc agaggccgca gctactcgca cggcatttgt
     9541 accaagtgcc acctgcattc cttttttcac atggcctaac gccacgtgcc ccatttcgtg
     9601 accgatcacc gcttcgactt cgttatccgt catcatatcc atcagcccgc tatagacgcg
     9661 gatacagccg ttagccattg caaaggcgtt cacatccttc gccatataca ctttgtaatt
     9721 taccggctga ccgttgatat tgttgcctag cgcattggca atagttgtca gacgtttagc
     9781 gtattcgcta ttggctggcg caatcgtcgc cttgctgtcc atctcctgac atgcctgatc
     9841 gctcagggtt ttcacctgcg catcactcaa actgtaagcc tgaaaagctt ccgctcctga
     9901 tgagagcagt ccgttggagt ccatattctg gcaaccagtc agtaccgttg ccacgctcat
     9961 tgctaccaat aaggcgcgaa ttttcatgtt ttctcttccg cacaaggtca ggattattct
    10021 aaaaatgcgc cgtttgcagg tgaatcgacg ctcagtctca gtataaggaa aagcgcagcg
    10081 gacgggcgag tagattgcgc aacatgcgag catgatccag agatttctga agcggcaaaa
    10141 ggatgttcca tgtacatgac gcgcggcttg cggtaaattg ttggcaaatt ttccggcgta
    10201 gcccaaaacg cgctgtcgtc aagtcgttaa gggcgtgccc ttcatcatcc gatctggagt
    10261 caaaatgtcc tcacgtaaag agcttgccaa tgctattcgt gcgctgagca tggacgcagt
    10321 acagaaagcc aaatccggtc acccgggtgc ccctatgggt atggctgaca ttgccgaagt
    10381 cctgtggcgt gatttcctga aacacaaccc gcagaatccg tcctgggctg accgtgaccg
    10441 cttcgtgctg tccaacggcc acggctccat gctgatctac agcctgctgc acctcaccgg
    10501 ttacgatctg ccgatggaag aactgaaaaa cttccgtcag ctgcactcta aaactccggg
    10561 tcacccggaa gtgggttaca ccgctggtgt ggaaaccacc accggtccgc tgggtcaggg
    10621 tattgccaac gcagtcggta tggcgattgc agaaaaaacg ctggcggcgc agtttaaccg
    10681 tccgggccac gacattgtcg accactacac ctacgccttc atgggcgacg gctgcatgat
    10741 ggaaggcatc tcccacgaag tttgctctct ggcgggtacg ctgaagctgg gtaaactgat
    10801 tgcattctac gatgacaacg gtatttctat cgatggtcac gttgaaggct ggttcaccga
    10861 cgacaccgca atgcgtttcg aagcttacgg ctggcacgtt attcgcgaca tcgacggtca
    10921 tgacgcggca tctatcaaac gcgcagtaga agaagcgcgc gcagtgactg acaaaccttc
    10981 cctgctgatg tgcaaaacca tcatcggttt cggttccccg aacaaagccg gtacccacga
    11041 ctcccacggt gcgccgctgg gcgacgctga aattgccctg acccgcgaac aactgggctg
    11101 gaaatatgcg ccgttcgaaa tcccgtctga aatctatgct cagtgggatg cgaaagaagc
    11161 aggccaggcg aaagaatccg catggaacga gaaattcgct gcttacgcga aagcttatcc
    11221 gcaggaagcc gctgaattta cccgccgtat gaaaggcgaa atgccgtctg acttcgacgc
    11281 taaagcgaaa gagttcatcg ctaaactgca ggctaatccg gcgaaaatcg ccagccgtaa
    11341 agcgtctcag aatgctatcg aagcgttcgg tccgctgttg ccggaattcc tcggcggttc
    11401 tgctgacctg gcgccgtcta acctgaccct gtggtctggt tctaaagcaa tcaacgaaga
    11461 tgctgcgggt aactacatcc actacggtgt tcgcgagttc ggtatgaccg cgattgctaa
    11521 cggtatctcc ctgcacggtg gcttcctgcc gtacacctcc accttcctga tgttcgtgga
    11581 atacgcacgt aacgccgtac gtatggctgc gctgatgaaa cagcgtcagg tgatggttta
    11641 cacccacgac tccatcggtc tgggcgaaga cggcccgact caccagccgg ttgagcaggt
    11701 cgcttctctg cgcgtaaccc cgaacatgtc tacatggcgt ccgtgtgacc aggttgaatc
    11761 cgcggtcgcg tggaaatacg gtgttgagcg tcaggacggc ccgaccgcac tgatcctctc
    11821 ccgtcagaac ctggcgcagc aggaacgaac tgaagagcaa ctggcaaaca tcgcgcgcgg
    11881 tggttatgtg ctgaaagact gcgccggtca gccggaactg attttcatcg ctaccggttc
    11941 agaagttgaa ctggctgttg ctgcctacga aaaactgact gccgaaggcg tgaaagcgcg
    12001 cgtggtgtcc atgccgtcta ccgacgcatt tgacaagcag gatgctgctt accgtgaatc
    12061 cgtactgccg aaagcggtta ctgcacgcgt tgctgtagaa gcgggtattg ctgactactg
    12121 gtacaagtat gttggcctga acggtgctat cgtcggtatg accaccttcg gtgaatctgc
    12181 tccggcagag ctgctgtttg aagagttcgg cttcactgtt gataacgttg ttgcgaaagc
    12241 aaaagaactg ctgtaattag catttcgggt aaaaaggtcg cttcggcgac cttttttatt
    12301 accttgatat gtccgtttgc ggacaagcaa tagataaggc gtgttgtaga tcacaaatat
    12361 ttatatgcaa taaatatcaa ttatgtaata tgcatcacga tatgcgtatt gacatttgtt
    12421 gttatatcta taactcaatg ttatataaga aattaataat tcactgtttt caaaacaccg
    12481 gtttccctgc tcaattgctt tcattaaacc gcacgagtat tttattttta ccgccggacg
    12541 gtaccctaaa aataaaataa ggataattta tgcggcttag tgattatttt ccagaatcat
    12601 caatctcagt tatacattca gcaaaagatt ggcaggaagc tatcgatttc tcgatggtat
    12661 cattgctgga taaaaactat atcagcgaga attacattca ggcaattaaa gattccacca
    12721 ttaacaatgg cccttattat attctcgcac caggcgtggc aatgcctcat gcgcgaccgg
    12781 aatgtggggc gcttaaaacc gggatgtctt tgacattact tgaacaaggt gtttattttc
    12841 cggggaatga cgaaccaatt aaattactca tcggactctc tgctgccgat gccgattcgc
    12901 acattggcgc tattcaggcg ttaagtgaat tactgtgcga agaagaaata ctcgaacaac
    12961 tcttaacagc atcatcagaa aaacaattag cggacattat cagccgcgga taattactct
    13021 ctcctctaca ggataatatt atggaaaaca agtctgctcg tgcaaaggtc caggcttttg
    13081 ggggcttttt gactgcaatg gtcatcccca atattggtgc ttttattgcc tggggtttta
    13141 ttactgcgtt atttattccc accggttggc tgcctaacga acatttcgcc aaaattgtcg
    13201 gcccgatgat tacctattta ttgcccgtga tgattggttc tacaggtggt catctggtcg
    13261 gcggtaaacg cggggcggtc atgggcggaa taggtactat tggtgtgatc gttggcgcag
    13321 agatcccgat gttccttggc tcaatgatca tggggccgct cggtgggttg gtcataaaat
    13381 atgtcgataa ggcactggaa aaacgcatac ctgccggttt tgagatggtt atcaataact
    13441 tctcattagg tatcgcgggg atgctccttt gtctgctggg ttttgaagtt atcggcccgg
    13501 cggtgttaat tgccaatact ttcgtcaaag agtgtattga ggcgctggta catgcgggtt
    13561 atctgccatt gttgtcagtc atcaatgaac cggcgaaagt gcttttcctc aataatgcga
    13621 tcgatcaggg cgtctattat ccgctgggaa tgcaacaggc ttcggttaac ggtaaatcca
    13681 tcttctttat ggtggcctct aacccaggtc cgggcctggg gctgctgctg gcgtttacct
    13741 tgtttggtaa agggatgagt aaacgttctg cgcccggggc gatgattatt cacttcctcg
    13801 gtgggatcca cgaactgtat ttcccgtatg tgctgatgaa gccgctgacc attattgcca
    13861 tgattgcggg cggtatgtct ggcacctgga tgtttaactt actggacggt ggtctggtgg
    13921 ctggcccaag tccggggtct atctttgctt acctggcact gacgccgaaa ggctcgttcc
    13981 tggcgacaat tgccggtgtt acggtaggta ccctggtgtc ctttgctatt acttcgctga
    14041 tactgaagat ggaaaaaacg gtggaaacgg agagcgaaga tgagtttgct cagtcagcca
    14101 atgcggttaa ggcgatgaaa caagagggtg cattctcgtt aagcagggtt aagcgtatcg
    14161 cctttgtttg cgatgcgggg atgggctcca gtgcgatggg cgcgaccacc ttccgtaaac
    14221 gcctggaaaa agcggggctg gcaattgaag taaaacatta cgccatagaa aacgtgcctg
    14281 cggatgcgga tatcgtcgtt actcatgcca gtctggaagg gcgcgtgaaa cgtgtgacgg
    14341 ataaaccact gatattgatt aataactata ttggcgatcc aaaactcgac actttattta
    14401 atcaattaac cgccgaacat aaacactgat tacagaggta aaaatgaaaa ccaaagttgc
    14461 tgctatttat ggcaagcggg atgtccgtct gcgcgtattt gaactgccag aaattaccga
    14521 taatgaatta ctggtgagtg taatttctga cagcgtctgt ttatcgacct ggaaagcggc
    14581 gttactcggt agtgaacata aacgcgtacc cgacgattta gaaaatcatc cggtcattac
    14641 cgggcatgaa tgtgccgggg ttattgtcga agtgggtaaa aatctcactg gcaaatataa
    14701 aaaaggccag cgttttgtat tgcaaccggc gatggggtta ccaagcggat attcagcggg
    14761 ctacagctac gaatattttg gcggcaatgc cacttatatg attattcccg aaatcgccat
    14821 taatttgggc tgcgtattac cgtatcacgg ctcttatttt gctgcggcgt cgctggcaga
    14881 gcctatgtgc tgcattattg gtgcttatca tgccaattat cacaccacgc aatatgttta
    14941 tgagcatcgc atgggcgtca aacctggcgg caatattgca ctgctggcgt gtgcaggtcc
    15001 gatgggcatt ggcgctatcg attacgccat taacggcggc atacaaccgt cgcgggtggt
    15061 ggtggtcgat atcgacgaca aacgtctggc gcaggtacag aagctgctgc cggtggaact
    15121 ggcggccagt aaaggcattg agctggtgta tgtgaatacc aaagggatga gcgatcctgt
    15181 ccagatgctg cgggcgctga caggagatgc cgggttcgat gacatttttg tttatgcggc
    15241 ggtgcctgct gtcgttgaga tggctgatga attactggcg gaagatggct gtctgaactt
    15301 ctttgccggg ccgacggata aaaacttcaa agtgccgttt aatttctaca acgtccatta
    15361 caacagcacg cacgtcgtcg gtacatctgg cggttcaacg gacgacatga aagaggcgat
    15421 tgcccttagc gccactgggc agttacagcc gtcgtttatg gtgacccata tcggtggcct
    15481 ggatgcggtg ccagaaaccg tgctcaatct gccggatatc cctggcggta aaaaactcat
    15541 ttataacggc gtgaccatgc cgctcactgc cattgccgat tttgccgaaa aaggcaaaac
    15601 cgatccgctg tttaaagagt tggcgcggct ggttgaggaa acgcacggca tctggaatga
    15661 acaggccgag aaatatctgc tggcacaatt tggcgttgat atcggggagg ccgcgcaatg
    15721 atgtccctgg cgtggccatt atttcgcgtt acggaacagg cagcgttggc tgcctggccg
    15781 caaaccggat gtggcgacaa aaataaaatt gatggcctgg cggtcaccgc gatgcgccag
    15841 gcattaaacg acgtcgcttt tcgtggacga gtggttatcg gcgaaggaga aattgaccat
    15901 gcgccgatgc tgtggattgg cgaagaggtg ggcaaaggcg atgggccaga agtcgatatc
    15961 gcggttgatc ccattgaagg cacgcggatg gtggcgatgg ggcagagcaa tgcgctggcg
    16021 gtcatggctt tcgcccctcg agatagcctg ttacatgcgc ccgatatgta tatgaaaaag
    16081 ctggttgtta atcggctggc ggcgggcgcg attgatctgt ctttgcctct gacggacaac
    16141 ctgcgcaacg tggcgaaagc gttaggcaaa ccgctggata agctgcgcat ggttacgctg
    16201 gataaaccgc gcctgagtgc cgcgattgag gaagcaacac agctgggcgt gaaggttttt
    16261 gccctgccgg atggcgatgt cgccgccagc gtgctgacct gctggcagga taatccttac
    16321 gatgtgatgt acaccatcgg cggtgcgcca gaaggcgtga tttctgcctg tgccgttaaa
    16381 gcattaggcg gggatatgca ggccgagctg attgactttt gccaggctaa aggggattac
    16441 acggaaaatc ggcagattgc agagcaggag cgcaagcgtt gcaaggcaat gggcgttgac
    16501 gttaaccgtg tgtactcgct cgatgaactg gtgaggggga acgatatcct ctttagcgct
    16561 acgggcgtga cgggcggtga gctggtgaac ggtatccaac agacggcgaa tggggtgcgg
    16621 acgcagacat tactgatcgg cggcgcggac caaacgtgta atataataga ctccctgcat
    16681 tgatggtgat ctatcggaag accaatggcg acgctgacag aagatgatgt gcttgagcaa
    16741 ctggatgcac aggacaattt attttcattt atgaaaactg cgcataccat tttgctccag
    16801 gggatacgcc agtttctgcc gtcgctgttt gtcgataacg atgaagagat cgtggaatat
    16861 gcagtgaagc cgttactcgc ccaaagcggc ccgcttgacg atattgatgt tgcactgcgt
    16921 ttgatttatg cgttggggaa aatggataaa tggttgtacg ccgatatcac gcatttttcc
    16981 cagttctggc attacctgaa cgaacaggat gaaacgcccg gatttgccga tgacatgacc
    17041 tgggatttta tcagcaacgt caatagcata acccgcaatg cgatgctcta tgacgcgtta
    17101 aaagcgatga agttcgctga tttctccgtc tggtcagagg cgcgttttag cggaatggtc
    17161 aaaacggcgc tgacgctggc agtaacgacc accttaaagg aattaacgcc gtgaaaattg
    17221 aattaacggt gaatgggctg aagatacagg cacagtacca gaacgaagag attgaaaatg
    17281 tacataaacc gttgctgcac atgttggcgg ccttacagac ggtaaatccg cagcggcgta
    17341 cggtggtttt tctttgcgct ccgccgggga cgggtaaatc aaccctaacc accttctggg
    17401 aatatctcgc gcaacaagac ccggaactgc ccgctattca gacgctcccg atggatggtt
    17461 ttcaccatta caatagctgg ctggatgcgc atcaattgcg ccccttcaaa ggcgcaccag
    17521 agacatttga cgttgcgaaa ctgacggaaa atctgcgcca ggtcgtggaa ggggattgta
    17581 cgtggccgca gtacgatcga caaaagcatg atcctgttga agatgcgttg cacgttaccg
    17641 caccactcgt catcgtcgaa ggaaactggt tgttactgga tgatgaaaag tggctggagt
    17701 tggcatcttt ctgcgatttt tctattttta ttcatgcacc tgctcaaatt ctgcgggagc
    17761 gcttgattag tcgtaaaatt gcgggaggat taacgcggca agtagccgaa gcattttatg
    17821 cccgcaccga tggccccaat gtcgagcgtg tgctgatgaa tagccgtcag gccaatctga
    17881 tagtagagat gactgaagaa ggccggtatc acttcacaag ctgacaattt attccagtta
    17941 ttgtagggtt attattacgc cagcctggaa taaagcttcc cacaaaaggg gataaaagtg
    18001 tgatgtgagt cagataaatg tcttcttcgg ctggacaaac attcctttta ttccacgttt
    18061 cgcttatcct agctgaagcg tttcagtcga ttaaatgttc gacaattaac caatcagtcg
    18121 cagtttgcga caggtaaggt ttccccggac gatttgctgg attactctgt ctggcaaatt
    18181 tgctcaggga aaaccttgca ggagatctat gaccgtacgc gtagcgataa atggcttcgg
    18241 tcgcatcggg cgtaatgtgg ttcgtgcttt gtatgaatcc ggacgccggg cggaaattac
    18301 cgtggtggca atcaacgaac tggcggatgc tgcgggcatg gcgcatttgt tgaaatatga
    18361 caccagccat ggccgttttg catgggaagt acgacaggaa cgcgatcaac tttttgttgg
    18421 tgatgacgcc atccgcgtat tgcatgaacg ttcactgcaa tcgctcccct ggcgtgaact
    18481 tggcgttgat gtagtcctcg actgcaccgg cgtatatggc tcccgcgagc atggcgaagc
    18541 gcatattgcc gccggggcca aaaaagtgct cttttcacat cctggcagta acgatctcga
    18601 cgcgaccgtt gtttacggcg tcaatcagga tcaacttcgt gcggaacacc gcatcgtttc
    18661 taacgcttcc tgtaccacga attgcataat tcccgtcatc aaattgttag atgatgcgta
    18721 cggtattgag tccggcactg tgaccacaat tcactccgcc atgcacgatc aacaggttat
    18781 tgatgcatac catcctgacc tgcgtcgcac ccgggcagcc agccagtcga tcattccggt
    18841 cgatactaaa ctggccgccg gtatcacacg attttttccg caatttaacg atcgctttga
    18901 agcgattgcg gtacgtgtgc caaccataaa tgtgacggca atcgatttaa gcgtgacggt
    18961 gaagaaacct gtaaaagcca atgaagtcaa cctgttgctg caaaaagcag cacaaggtgc
    19021 atttcatggt atagttgact atacggaatt gccgttggtc tctgtagatt ttaaccacga
    19081 tccgcacagt gccattgtcg atggcaccca aacccgggtc agtggcgcac acctgatcaa
    19141 aacgttggtc tggtgcgata acgaatgggg ctttgctaac cgaatgctcg acacgacgtt
    19201 agctatggct actgttgctt tcaggtaaga cgcaagcagc gtctgcaaaa cttttagaat
    19261 caacgagagg attcaccatg tctgtaatta agatgaccga tctggatctt gctgggaaac
    19321 gtgtatttat ccgtgcggat ctgaacgtac cagtaaaaga cgggaaagta accagcgacg
    19381 cgcgtatccg tgcttctctg ccgaccatcg aactggccct gaaacaaggc gcaaaagtga
    19441 tggtaacttc ccacctgggt cgtcctaccg aaggcgagta caacgaagaa ttctctctgc
    19501 tgccggttgt taactacctg aaagacaaac tgtctaaccc ggttcgtctg gttaaagatt
    19561 acctcgacgg cgttgacgtt gctgaaggtg aactggttgt tctggaaaac gttcgcttca
    19621 acaaaggcga gaagaaagac gacgaaaccc tgtccaaaaa atacgctgca ctgtgtgacg
    19681 tgttcgtaat ggacgcattc ggtactgctc accgcgcgca ggcttctact cacggtatcg
    19741 gtaaattcgc tgacgttgcg tgcgcaggcc cgctgctggc agctgaactg gacgcgctgg
    19801 gtaaagcact gaaagaacct gctcgcccga tggtggctat cgttggtggt tctaaagtat
    19861 ctaccaaact gaccgttctg gactccctgt ctaaaatcgc tgaccagctg attgttggtg
    19921 gtggtatcgc taacaccttt atcgcggcac aaggccacga tgtgggtaaa tccctgtacg
    19981 aagctgacct ggttgacgaa gctaaacgtc tgctgaccac ctgcaacatc ccggttccgt
    20041 ctgatgttcg cgtagcaacc gagttctctg aaactgcacc ggctaccctg aaatctgtta
    20101 acgatgtgaa agctgacgag cagatcctgg atatcggtga tgcttccgct caggaactgg
    20161 ctgaaatcct gaagaatgcg aaaaccattc tgtggaacgg tccggttggc gtgttcgaat
    20221 tcccgaactt ccgcaaaggt actgaaatcg tggctaacgc tatcgcagac agcgaagcgt
    20281 tctccatcgc tggcggcggc gacactctgg cagcaatcga cctgttcggc attgctgaca
    20341 aaatctccta catctccact ggcggcggcg cattcctcga attcgtggaa ggtaaagtac
    20401 tgcctgcagt agcgatgctc gaagagcgcg ctaagaagta aaaaatcaca gggcagggaa
    20461 acctgccctt gtttcagcgc gcttttagag cacgcaattt ttcatactct aaataattcg
    20521 agttgcagga aggcgacaag agagtgaatc cccaggagct tacataagta agtgactggg
    20581 gtgagcgaac gcagacgcag cacatgcaac ttgaagtatg acgagtataa ggcccgacga
    20641 tacaggacaa gagacatgtc taagattttt gatttcgtaa aacctggcgt aatcactggt
    20701 gatgacgtac agaaagtttt ccaggtagca aaagaaaaca acttcgcact gccagcagta
    20761 aactgcgtcg gtactgactc catcaacgcc gtactggaaa ccgctgctaa agttaaagcg
    20821 ccggttatcg ttcagttctc caacggtggt gcttccttta tcgctggtaa aggcgtgaaa
    20881 tctgacgttc cgcagggtgc tgctatcctg ggcgcgatct ctggtgcgca tcacgttcac
    20941 cagatggctg aacattatgg tgttccggtt atcctgcaca ctgaccactg cgcgaagaaa
    21001 ctgctgccgt ggatcgacgg tctgttggac gcgggtgaaa aacacttcgc agctaccggt
    21061 aagccgctgt tctcttctca catgatcgac ctgtctgaag aatctctgca agagaacatc
    21121 gaaatctgct ctaaatacct ggagcgcatg tccaaaatcg gcatgactct ggaaatcgaa
    21181 ctgggttgca ccggtggtga agaagacggc gtggacaaca gccacatgga cgcttctgca
    21241 ctgtacaccc agccggaaga cgttgattac gcatacaccg aactgagcaa aatcagcccg
    21301 cgtttcacca tcgcagcgtc cttcggtaac gtacacggtg tttacaagcc gggtaacgtg
    21361 gttctgactc cgaccatcct gcgtgattct caggaatatg tttccaagaa acacaacctg
    21421 ccgcacaaca gcctgaactt cgtattccac ggtggttccg gttctactgc tcaggaaatc
    21481 aaagactccg taagctacgg cgtagtaaaa atgaacatcg ataccgatac ccaatgggca
    21541 acctgggaag gcgttctgaa ctactacaaa gcgaacgaag cttatctgca gggtcagctg
    21601 ggtaacccga aaggcgaaga tcagccgaac aagaaatact acgatccgcg cgtatggctg
    21661 cgtgccggtc agacttcgat gatcgctcgt ctggagaaag cattccagga actgaacgcg
    21721 atcgacgttc tgtaagatat tcctttctgc ttatctcaag gcccgctctg cgggtctttt
    21781 tttcgccaaa agcaaaattg cctgatgcgc tacgcttatc aggcctacac gtatcctgca
    21841 atatattgaa tttgcaggtt tttgtaggcc ggataaggcg ttcacgccgc atccggcatg
    21901 acaaagcgca cattgatatc tgtctgagcc ccgctctgcg gggatttttt cgccaaaagc
    21961 aaggtgattc agatgagaaa tctgtgatct atttggcaaa attatgcttt attgtttacc
    22021 cttgtcagac tgcccgtcat aaggcggcgg agtgtatttc tccattttga gtcagttgaa
    22081 aaggaatatt gaatggaaga tttgaatgtt gtcgatagca taaacggcgc gggaagctgg
    22141 ctggtagcta accaggcgct gctgctaagt tatgcagtaa acatcgtggc ggcactcgcg
    22201 atcatcatcg ttggtttgat tatcgcgcgg atgatttcca acgcggtgaa tcgcctgatg
    22261 atctcccgta aaatcgatgc cactgttgct gattttcttt ctgcattagt ccgttacggt
    22321 attatcgcct ttacgctaat cgctgcactg ggacgcgtgg gtgtacaaac cgcgtcagtc
    22381 attgctgtac tcggtgccgc aggcttagct gttggtctgg ctttgcaggg gtcactgtct
    22441 aacctggccg ctggcgtgtt acttgtcatg ttccgcccgt tccgtgccgg agaatatgtt
    22501 gacctgggcg gcgtagccgg tactgtgctg agtgtgcaga ttttctccac caccatgcgt
    22561 actgcagacg gtaaaattat cgttattccg aacggtaaaa ttattgccgg aaatattatt
    22621 aacttctccc gcgagccagt tcgccgtaac gaatttatta ttggcgtggc gtatgattcc
    22681 gatatcgatc aggttaagca gatcctgacc aatattatcc agtctgaaga tcgcattttg
    22741 aaagatcgcg aaatgactgt gcgcctgaac gaacttggtg catcgtcgat taatttcgtg
    22801 gtccgcgtct ggagcaacag cggcgatctg caaaacgtgt actgggatgt gctggagcgt
    22861 attaaacgtg aatttgatgc cgccggtatc agcttcccgt acccgcaaat ggatgtgaac
    22921 tttaagcggg tgaaagaaga caaagctgcg taatcaacgc tgaggccaga taatactctc
    22981 tggcctctct cttattagtt tttctgattg ccaattaata ttatcaattt ccgctaataa
    23041 caatcccgcg atatagtctc tgcatcagat acttaattcg gaatatccaa cgtgttttct
    23101 tattactttc aaggtcttgc acttggggcg gctatgatcc taccgctcgg tccacaaaat
    23161 gcttttgtga tgaatcaggg catacgtcgt cagtaccaca ttatgattgc cttactttgt
    23221 gctatcagcg atttggtcct gatttgcgcc gggatttttg gtggcagcgc gttattgatg
    23281 cagtcgccgt ggttgctggc gctggtcacc tggggcggcg tagccttctt gctgtggtat
    23341 ggttttggcg cttttaaaac agcaatgagc agtaatattg agttagccag cgccgaagtc
    23401 atgaagcaag gcagatggaa aattatcgcc accatgttgg cagtgacctg gctgaatccg
    23461 catgtttacc tggatacttt tgttgtactg ggcagccttg gcgggcaact tgatgtggaa
    23521 ccaaaacgct ggtttgcact cgggacaatt agcgcctctt tcctgtggtt ctttggtctg
    23581 gctcttctcg cagcctggct ggcaccgcgt ctgcgcacgg caaaagcaca gcgcattatc
    23641 aatctggttg tgggatgtgt tatgtggttt attgccttgc agctggcgag agacggtatt
    23701 gctcatgcac aagccttgtt cagttaggcg ctatctgatg gaaaaataaa acagaggcgc
    23761 taagcttgcc tccagaggtc ctgaattttg ggcaagtcaa ttaacacgga gagactaacg
    23821 tgaagttcaa agttatcgcc ctggcggcat taatgggtat tagcgggatg gcagcgcagg
    23881 ctaacgaatt gccggatgga ccgcatattg tcacctccgg tacggcaagc gtggatgcgg
    23941 tgccagacat tgccactctt gcgattgaag ttaacgtggc cgcgaaggat gccgctactg
    24001 ccaagaaaca ggcagatgag cgcgtcgcac aatacatttc cttccttgaa ctcaatcaga
    24061 tcgcgaaaaa agatatcagc tcagcgaact tacgcaccca gccagattat gattatcagg
    24121 atggtaaaag tatccttaaa ggctaccgcg ctgtgagaac ggtggaagtc acgctccgtc
    24181 agttagacaa actgaattcc ttgctggatg gcgcgctgaa ggcgggtctt aacgaaattc
    24241 gttctgtgtc gctgggcgtg gcgcagccgg atgcctataa agacaaagcg cgtaaggcag
    24301 cgattgataa cgcgattcat caggcgcagg aactggcgaa cggctttcat cgtaaactgg
    24361 ggccggtata tagcgtgcgc taccatgttt ccaactatca gcccagccca atggtgcgga
    24421 tgatgaaagc cgatgccgcg ccggtgtccg cccaggaaac ttacgagcag gccgctattc
    24481 agtttgatga tcaggtcgat gtggtcttcc agttagaacc tgtggatcaa caacccgcta
    24541 aaacacctgc agcacaataa tatttgtagc ccgacgtatt cgtcgggcta atgcctttac
    24601 ccgatattgc ctcctttttc tgtaattttc tgtaaaacca tgacgtaatc acctatcttt
    24661 gctcgcggtt tcatcaagga taggctgact gaaacggtta agcggacaag gatgcggggg
    24721 agggggatgg acatttttat ttcgaaaaag atgcgcaatt tcattttatt agcgcaaacc
    24781 aataatattg cccgggcggc agaaaaaatc catatgacgg cttcgccatt tggtaaaagc
    24841 attgccgcgc tggaagaaca aattggctat acgctattta cccgcaaaga taataatatc
    24901 agcctcaata aagccggaca ggaactgtat caaaaactgt tcccggttta tcaacggctt
    24961 tctgcgattg ataatgaaat ccataactca gggcgtcgtt cacgagaaat tgtgattggc
    25021 atagataata cttatccgac aattattttc gatcagttga ttagtctcgg agataagtat
    25081 gaaggcgtga ccgcccagcc agttgagttc agcgaaaatg gcgttatcga taatcttttt
    25141 gaccgccagt tagattttat tatctcccca tagcatgtat ctgcacgagt tcaggaactg
    25201 gaaaacctga ctatcagtga actaccaccg ctgcgattag gctttctcgt ttctcgccgt
    25261 tatgaagagc gacaagagca agagctgctt caggagctgc cctggctaca aatgcgcttt
    25321 cagaaccggg ccaacttcga agcaatgatt gatgcgaata tgcgaccttg tgggatcaat
    25381 ccaaccatta tttatcgtcc ctacagtttt atggccaaaa tcagcgccgt ggagcgcgga
    25441 cattttctga cggtgatccc gcattttgcc tggcgactgg tgaatccggc aacgctgaaa
    25501 tattttgatg caccgcacag gccgatgtat atgcaggaat atctttattc aatcagaaat
    25561 catcgttata ccgccacgat gcttcagcat attgctgaag atcgtgacgg gacgagtcat
    25621 taacccagca tcgagccggt tgcaattaaa ttacggtgta agtcgaagac gtggctaaga
    25681 tcgtggtgaa tatgtccgcc aggcgcattt tccagatagc gatgcagata atcccgatac
    25741 ataggatgtg cacaattatc aatgatagtg cgggcgcgtt gaagcgggga aagaccgcgc
    25801 agatcggcga tcccttgttc ggtgatgatc actttgacgc tgtgttcgct gtgatcaaca
    25861 tggctgcaca ttggcacgac ggttgagatc ttgccttctt tagcaatcga cggggccata
    25921 aagatcgaca gatacgcgtt gcgttcaaaa tcaccgctgc cgccgatgcc gttcatcaga
    25981 tcgaccccgg ctacgtgtgt tgagttggca tgcccgtaaa tatcaaactc caggccgacg
    26041 ttcagagcga tgacgcccag acgacggatg atttccgggt tattggaaat ctcctgcgga
    26101 cgcaacacaa tgcggctggc aaagtaatcc atattgtcgt aaatcttgcg cagggaatcg
    26161 gccgagattg tcaggctgga ggcgctggcc ccgctgattt tgccggtttc cagtaaatgc
    26221 accaccgatt cctgtagcac ttccgaatac atcataaacg gaggaattac cgggttttcc
    26281 cccagacgcg ccattaccgc attattgata ttgcccacgc cactttgcag cggcagaaat
    26341 tccggcggaa tacgcccatg cgccatttcc tgcaataaga acgtgaccac gttatcggca
    26401 atctgctggc acatgggatt ttgcttatcc agcatattac cggcgtcggg caagttggtt
    26461 tccacgacgg cgacaatctt tttcggatcg atttgcacat agcgggtacc gacgcgatcc
    26521 attgcatgga agatcgacac gctattgcgc cgcggtggcg cgccaggaat cacaatatcc
    26581 gccagttctg caacgcgcgg atcgtgatag tgattgagtt caatgatcac tttcttcgcc
    26641 cgcagcagcc aggtcggcgc attaccgatc ccgctggtta accagactcg accatccggt
    26701 gccagtgccg atgcttcaat gacggcaaca tcaatgtcgc cgaagaaacc gtaattgacc
    26761 atttgcgcca cttcgctcaa atgcaggtca acgaaactca ccgcgccctg attgatcttt
    26821 ttacgtaaac cggacgatgt ttgatatggc gcacgccagg aaacagcatc ggcgtcagaa
    26881 agtacatcgt cagcggcggc gctgattgac gcacccgtca gaaggcgaat ttgatacggc
    26941 tttttggcct catgctgttc gttagctctg cgggcaatcg cggtgggtag ggctttcggc
    27001 gaacccgccg gggtaaagcc gctaaatgcc accatgtcgt tatgctggat aatttctgcc
    27061 gcttcattgg cggtcatcct tgtccactga gtttccattt ttactccttc gttcgcaggg
    27121 attaatgacc aacgaaatta ggtttacgtt tttcgaggaa agcgttcatc ccttcctggt
    27181 aatcttcgct gtcatacacc gcgcggcgca tcccctgaat acgttcaaat tcatcggagt
    27241 tcatggtgtg tgcttcgccc agtacacgca gctcttcttt gataacggca atggctaacg
    27301 gcgctttctc agagatgtgg tgcgccattt gtaaggtgaa atcttccagt tcttccactt
    27361 ccacaacatg gttgaggatg ccgacagcca gcgcgcgctg ggcggtgatt ggcgaagcgg
    27421 taaaaatcag ctctttgaca atgtggaagc ccgcgtcgcg ggtcaggttg tgaatgccga
    27481 ccaggttata cgggacgccg aggtttacag gcgtcattga gaaggttgag gtactggcgg
    27541 cgatgatcag atcggaactc atgatcattt caaatgcgcc accccaaaca ctaccttcca
    27601 ccatcgaaat gatcggtttc gggaattttt ggatcatgcg ggtgatttga cgcaatggat
    27661 catcatagga gagcggatcg cgaccgccag acggcagttc gtgaatatcg tgacctgcgg
    27721 agaagacttt ggatccactc ggtgcgcgca aaatgataca gcgaatttcc ggccggttga
    27781 gatcgcttaa cgcctgcata agatcatcaa taaagacttt acttaaggca ttaagttttc
    27841 ggccatagtt aaactcaatg accgccactt tgttgatagt gacaacgtta acatactgat
    27901 aagacataaa aattccttta atcaaaatat tgcgtctgga taaattcact gagctgccgc
    27961 aggccggtgc gcggtgagag cgtattgttt ttgaccgcta aaagcgtctg gcggtaatag
    28021 cgatcgaaat cttcattcgc gaacaggtga ttcagtactt cttcttcggt ctgcttacgc
    28081 agccattcca ccgattgttg ttgccgcact tgttgtaaac gaccactggc agttagcgcg
    28141 gttttgaagt cgatgatggc gtgccagatc tcatcgattc cacgtttttc cagtgcgcta
    28201 caagtcagaa cccgtggctg ccattcgtcg tatttacgtc gcagaatatg cagggcactc
    28261 tcgtacatat gccgggcaat ggcgacattg gtatggttat cgccatcgtc tttgttgata
    28321 acgatcagat cagccacttc catcagccct tttttaatgc cctgcagatc atcgccgcca
    28381 ccggcaattt gcaacgagat aaaacagtcc accatgcggg cgacttctgt ttccgactgc
    28441 ccgacgccaa ccgtttcgac aatcactacg tcataacccg ctgcttcgca taacagcatt
    28501 aattcccgcg ctcgctgact ggcaccgccc agatgaccgg aggatggtac cgggcgaata
    28561 aacgccgctt cggcacgcgc caggtcattc atgcgggttt tatccccgag aatgctaccg
    28621 ccagtgaccg ggctgctggg atcgaccgca ataaccgcga cctttaatcc ctctcgaatc
    28681 aacaacatgc caaaggcctc aagaaaggta cttttccccg cgccgggggt gccggtaacg
    28741 cccagtcgca gggtgttacc gcagtacggc ataatggcat caagcagctg cgtacttagt
    28801 gcctgatgac gcgggtgacg gctttccacc agcgtcatgg cctgggcgag tgtggcacgc
    28861 tcaccctgac gtaagcggcg aatactttct gccagcgtgg cttcattaat catgatgctg
    28921 gcttatcaga ttcagtacgt cgcgcacact gtcgagcata ggtgtacctg gaccataaat
    28981 cgccgccacg ccgcgctctt gcaggaaggc gtaatcctgc ggcggaatga cgccacccgc
    29041 gaccacgcag atatcttcgc gtccccattt tttcagcgct tcgaccagtt ccgggatcag
    29101 cgttttatga ccggcagcca gtgaggatgc gcccactacg tgaacgtcgt tttctacggc
    29161 caggcgggcg atctcttcag gtgtagagaa catcgggctt aaatctacgt cgaaaccgag
    29221 atcggaatag gcgctggcga tcactttcgc gccgcgatcg tgtccatcct ggcccatctt
    29281 agcgatcaga atgcgcgggc gacgaccatt gtcggcaagg aactgctccg tttgcgcaac
    29341 aatggcatcg aactcggagg ccgatttctc agactgatga tagctttgcg caatcacgcc
    29401 ggtaacacac tggcttggca ccagataacg gtcgaaagcg acttccagcg catcggaaat
    29461 ttcacccagg gtggcgcgaa cgcgagcggc attaacagcg gcagccagca ggttttcgtt
    29521 atgctgtgcg gcgtgagtca gggcgttcaa cgcggcggtt acggcggcat catcacgggt
    29581 ggcgcgaatg cgttccagcg aagcaatttg ctcgttacgc accatcacgt tgtcgatctc
    29641 aagtacatcg gtttcgtctt cgtgatccag tttgtacttg ttgacaccaa cgatgacacg
    29701 cttgccctgg tcgatcagcg actgttcgcg cgctgaggcc tcttcgatca ttcgttttgg
    29761 cagacctgct tcgatcgctt tcgccatgcc accggcttcg tcgatctgtt ggataatagc
    29821 tctggcttgt ttgacgattt gatcggtcag cgactcaatg taataggatc cggccagtgg
    29881 atcgacggtg cggcagagtt ctgattcttc ctggatgatg atctgggtgt tgcgggcaat
    29941 gcgtgctgag aaatcggtag gcaaaccaag cgcttcgtca aaggcgttgg tatgcagtga
    30001 ctgagtaccg cccagcgtcg cagccagcgc ttcaatggtg gtgcggataa cgttgttata
    30061 cggatcctgt tcagtcaggc tccagcctga ggtctggcag tgggtacgca gcgccagtga
    30121 tttcgggtcc tgtgcgccaa atccactgac cgcttcgctc cataaataac gtgccgcacg
    30181 caacatggcg acgttcataa acagatccat gccgatgccg aagaagaacg acaggcgagg
    30241 agcgaagtca tcaattttca gtccggcaga gattgctgct ttgatgtact caatcccatc
    30301 agcgagcgta aatgctacct gctgcacgca gttggcaccc gcttcaccca tgtggtaacc
    30361 gctgatactg atggtattaa atcgcggcat gttgccggaa caccaggcga tgatgtcggc
    30421 gataatgcgc attgacggtt ttggtgggta aatataggtg ttgcggcaga ggtactcttt
    30481 gagaatatcg ttttgaatgg tgccggtcag tttatcaggt gtaacacctt gctcttctgc
    30541 ggcgacgata taaaacgcca gtactggtag cactgcgcca ttcatggtca tcgaaaccga
    30601 cattttatcc agcgggatct ggtcgaacag gactttcata tcttccacgg tgtcgatagc
    30661 gacgcccgct ttgccgacgt cgcccgccac gcgcgggtta tcggagtcgt agccacggtg
    30721 ggtggcaagg tcaaacgcaa cggaaagacc tttttgcccg gcggccaggt tacggcgata
    30781 aaaagcgttg gactcttttg ctgttgaaaa accagcatac tgacggatgg tccacggttg
    30841 ggcggtatac atagtggcac gcgggccacg aacgtagggc ggcaaaccag gaagggtacc
    30901 tgtcacctcc agattatcga gatcggcttc ggtatacagc ggcttgatgg cgatcccttc
    30961 cgcggtttga tgaaccagcg agtcgacagt tttctcccga cggctcaatt ccttgttggc
    31021 aagctgttgc cactcctgca cgttagacat tatttgctcc gtaaaacgca atcaaaatga
    31081 gggtatttgc gcctacggtg agctttttac gtgggtttgt cgccagcaaa aaaaatgcat
    31141 tcagacgtcg ttttttgccg cctgagattt gtctattttg tgatgtgatt caaggaatac
    31201 aaaaagaagt gaggacaacg cctgatatgt gctatcaggc attatttgat ggattaatcc
    31261 tgacgaagga ctttgtgacc ataatcgagt aacgcatcag tgactttacg catcatgcgg
    31321 ctttcaggag caaagcggtg ccagtagagc atccgtcgtt gaaatagccc aggcgttaag
    31381 tcaatcagtt caccgctggc cagctctttc tcgatttgca ggtgcgggat catacagcag
    31441 gtggtgccct ggcgagcaag ttgtacgaac gcttctgaag aattaacgat atggcagggc
    31501 acgctgcctg gaggcagatc gaagttttgc tgcaaaaagg cctggtgcat atcgtcaaga
    31561 tggtcaaacg cgaccactgg cgctttcagt aatgccgaac gcgttacgcc gttagggaaa
    31621 tatttttcgg caaagggttt tgagctgacg aacagatagt cgagcgcacc aagtttatcg
    31681 acaagacaac tcggcagcgc ctgatgttga atactcaccg cgccgaccac ttcgccgcgg
    31741 cgcagacgtt cctgagtgcg ggtttcatct tctacctgca agttgaggcg gataggcgaa
    31801 tcagccaaca caggagccag tgcaggaagc aaccacgtcg ccagactgtc ggcgttgacc
    31861 gccagtgaaa gcagcagcgg agtcgaaccg gtttgttcat cgcccagcca ctcttcttcc
    31921 agcaactcca cctggcgcag cagtgccagc agtttttgcc cttgttccgt cgggcgcggc
    31981 ggtacggtac gcaccaacag cggctgcccg aacatatttt ccagttgctt aatgcgctgt
    32041 gagacggctg attgtgtaat gcacagcttt tgtgccgcgc gctcaaatcc tcgttcacgt
    32101 atcaccgcat ccagtgcctg taatgttctg tagtccgggc gtttcattgc tgttgtctgc
    32161 tcctggtaaa tgctttttcc tgcactatga cacaattttg tgtcaggttg catataccga
    32221 agccgtcggc gggtaacatt tgtatccgtg gttaaaagcg cagcttaaaa gatcagggat
    32281 ttgcgctaat gcatttcgct cagcgagttc gcgccctggt tgtgttgaat ggcgtggcgt
    32341 tattgcctca atttgcctgt aaacaggggc ttgcgaacgg tgaactggtg cgcctgtttg
    32401 caccgtggag cggcataccc agaccgttgt atgctttatt tgcggggcga aaggggatgc
    32461 ctgccattgc gcgatatttt atggatgagt taaccacgcg gcttgccaac ggggtctgaa
    32521 tcgctttttt tgtatataat gcgtgtgaaa tttcatacca caggcgaaac gatcatgacg
    32581 caggatgaat tgaaaaaagc agtaggatgg gcggcacttc agtatgttca gcccggcacc
    32641 attgttggtg taggtacagg ttccaccgcc gcacacttta ttgacgcgct cggtacaatg
    32701 aaaggccaga ttgaaggggc cgtttccagt tcagatgctt ccactgaaaa actgaaaagc
    32761 ctcggcattc acgtttttga tctcaacgaa gtcgacagcc ttggcatcta cgttgatggc
    32821 gcagatgaaa tcaacggcca catgcaaatg atcaaaggcg gcggcgcggc gctgacccgt
    32881 gaaaaaatca ttgcttcggt tgcagaaaaa tttatctgta ttgcagacgc ttccaagcag
    32941 gttgatattc tgggtaaatt cccgctgcca gtagaagtta tcccgatggc acgtagtgca
    33001 gtggcgcgtc agctggtgaa actgggcggt cgtccggaat accgtcaggg cgtggtgacc
    33061 gataatggca acgtgatcct cgacgtccac ggcatggaaa tccttgaccc gatagcgatg
    33121 gaaaacgcca taaatgcgat tcctggcgtg gtgactgttg gcttgtttgc taaccgtggc
    33181 gcggacgttg cgctgattgg cacacctgac ggtgtcaaaa ccattgtgaa atgatctgac
    33241 gggggaacct cccccgttaa aaaaattctc ttcattaaat ttggtgacat gtgtcacgct
    33301 tttaccaggc aattgtcgat tgctctaaat aaatcctcta aaccagcata ttcatccaag
    33361 aattaccttt gcgtgatatt tcctcaacat cgcgacgcaa acgttcatat tgccgcaata
    33421 ttattttttg atatgttgaa aggcggatgc aaatccgcac acaacatttc aaaagacagg
    33481 attgggtaaa tggcaaaggt atcgctggag aaagacaaga ttaagtttct gctggtagaa
    33541 ggcgtgcacc aaaaggcgct ggaaagcctt cgtgcagctg gttacaccaa catcgaattt
    33601 cacaaaggcg cgctggatga tgaacaatta aaagaatcca tccgcgatgc ccacttcatc
    33661 ggcctgcgat cccgtaccca tctgactgaa gacgtgatca acgccgcaga aaaactggtc
    33721 gctattggct gtttctgtat cggaacaaac caggttgatc tggatgcggc ggcaaagcgc
    33781 gggatcccgg tatttaacgc accgttctca aatacgcgct ctgttgcgga gctggtgatt
    33841 ggcgaactgc tgctgctatt gcgcggcgtg ccggaagcca atgctaaagc gcaccgtggc
    33901 gtgtggaaca aactggcggc gggttctttt gaagcgcgcg gcaaaaagct gggtatcatc
    33961 ggctacggtc atattggtac gcaattgggc attctggctg aatcgctggg aatgtatgtt
    34021 tacttttatg atattgaaaa taaactgccg ctgggcaacg ccactcaggt acagcatctt
    34081 tctgacctgc tgaatatgag cgatgtggtg agtctgcatg taccagagaa tccgtccacc
    34141 aaaaatatga tgggcgcgaa agaaatttca ctaatgaagc ccggctcgct gctgattaat
    34201 gcttcgcgcg gtactgtggt ggatattccg gcgctgtgtg atgcgctggc gagcaaacat
    34261 ctggcggggg cggcaatcga cgtattcccg acggaaccgg cgaccaatag cgatccattt
    34321 acctctccgc tgtgtgaatt cgacaacgtc cttctgacgc cacacattgg cggttcgact
    34381 caggaagcgc aggagaatat cggcctggaa gttgcgggta aattgatcaa gtattctgac
    34441 aatggctcaa cgctctctgc ggtgaacttc ccggaagtct cgctgccact gcacggtggg
    34501 cgtcgtctga tgcacatcca cgaaaaccgt ccgggcgtgc taactgcgct gaacaaaatc
    34561 ttcgccgagc agggcgtcaa catcgccgcg caatatctgc aaacttccgc ccagatgggt
    34621 tatgtggtta ttgatattga agccgacgaa gacgttgccg aaaaagcgct gcaggcaatg
    34681 aaagctattc cgggtaccat tcgcgcccgt ctgctgtact aattcccctt ctctgaaaat
    34741 caacgggcag gtcactgact tgcccgtttt tttatccctt ctccacaccg ttgccgcgtt
    34801 gcgcattcta ctttgcgagt ctgctccaga aaataaatca aagtcagatc aatgcgttgt
    34861 gtttatttaa tggggattta cgatggcagg gcagcatggg gctgtaacgg taaagccctc
    34921 accgaagcga gggcttgaag gagaagggtt atgatgcgac ttgtcatcat actgattgta
    34981 ctgttactca taagtttcag cgcttattaa cagtcagtct caggggagga gcaatcctcc
    35041 cttaccctta ctcactaaat taggtcaaag aatcaacgat gtcaatcagg gcgatgcggg
    35101 tgtatcgccc ttaccactcc cagactttcg acggtgtaac caccgcagga agagggatat
    35161 cccactcttc aacggggagt ttttccacca actgacaatc atgcgcataa cccaccggtt
    35221 gcgttttata gtgctgccag ttttgtaagg tccgatcata aaaaccaccg cccattccca
    35281 ggcgctgacc gtactcatca aaggcgacca gcggtgtgat cagcacgtct aatcgggaaa
    35341 ggggtagcac gtcacgcaca tccaattttg gctcatggat cttcaacctg ttcatcacca
    35401 gttcgctttg cggatggtaa ttcaggaaca gcaaattacc ggcactaaag ggatgcaaaa
    35461 ctggaagata tacgcgctta ccggcgcgcc agagttgttc tatgagtggc tgggtgtcga
    35521 gttcgccatc aaaagagagg aatacagcga ccgtatgtgc catcaccacc gggggataag
    35581 tcatcatccg ggtagcggct tgttgaccca tttcctgctg ttgttccggc gttaacgcac
    35641 gacgacgttg ccgaatcatt ttgcggattt cttgtcggga taatgtcagt gggagttctg
    35701 gtagttgcgt catggctggt gccagataag aagggaatct ccgagatgcc gccgcaggct
    35761 gtaacccttg aacccttggt tcaaggtgaa tgtgtcgtcg cagttttaag gcttctcgga
    35821 cggaccgagc atgctcacca accgcggagc gccacattct tgtggtatga aatatcggct
    35881 caggggactg gcccgcttgc gaacatctca gagaaatttt gtcttcacgg ttactctacc
    35941 acagtaaacc gaaaagtgtc attcaaagtt ttggttagtt ttttcggtga tgcgaccttg
    36001 ttcaagtaac gcttgttcta tggtctgctg cagcatccga atacgctgtt ccatacttgc
    36061 cgcgtagtca cgagtctttg ctttttcttg cgctaactca tagctgatat tcaatgcggc
    36121 aatgaagacc aactgttcag tatttgtgac tctagtgcgt tctttcagat cttgcaaccg
    36181 ttggttcaga tcgtccgctg cctgattcaa cgcatccctt tggtcaggcg ggcagttcac
    36241 acgcagtgaa cggccaaaaa tttggatatc gacgggttgt gcagacatgc caccttcctg
    36301 ctgattgact gcgctgcctt cgtctttaga cccatggtct gcgaaggggc gacactatag
    36361 ctaccctgat gagaagagac aagccctttt ctggtccacc agggcccaaa gtggtagcat
    36421 atcatgaata ttcctccctt tgacgacgaa tgcttatgtc tatacagaac gaaatgcctg
    36481 gttacaacga aatgaaccag tatctgaacc aacaagggac gggtctgacc ccagctgaga
    36541 tgcatggttt aatcagcggg atgatatgtg gcggtaacga tgacagctca tggctaccgc
    36601 tacttcacga cctgacgaac gaaggcatgg ctttcggtca tgagctggca caggcactgc
    36661 gtaaaatgca ctctgccacc agcgatgccc tgcaggatga cggcttcctt tttcagcttt
    36721 atctgcctga tggcgatgat gtcagcgttt tcgatcgggc tgatgcattg gcaggttggg
    36781 tcaatcactt cctgcttggt cttggcgtta cgcaaccgaa gctggataaa gtgaccggcg
    36841 aaaccggtga agctatcgac gatctgcgta acattgcgca actgggttac gacgaagacg
    36901 aagatcagga agagcttgaa atgtcgcttg aagagatcat cgaatacgtt cgtgttgccg
    36961 cgctgttatg ccacgacacc tttactcatc cgcaaccgac cgcgccagaa gtacaaaaac
    37021 cgactctaca ctaaaaacaa aaaacgtaag gagagtgtta tgagtgagat atcccggcaa
    37081 gagtttcagc gtcgccgtca ggccctggtg gagcaaatgc aacccggcag cgccgcgctg
    37141 atttttgctg caccagaagt aacacgtagc gccgacagcg aataccccta tcgtcagaac
    37201 agtgacttct ggtacttcac cggctttaac gaaccggaag cggtgctggt gctgattaaa
    37261 agcgatgaca ctcataacca cagcgttctg tttaaccgcg ttcgcgacct gacggcggag
    37321 atctggtttg gccgtcgctt aggccaggat gccgcgccag agaaactggg cgttgaccgc
    37381 gcactggcat tcagcgaaat caatcagcaa ctttatcaac tacttaacgg cctggatgtg
    37441 gtttaccatg cccagggcga atatgcatat gctgatgtaa tcgtgaacag tgcgctggaa
    37501 aaactgcgta aaggttcgcg gcaaaatctc accgcaccgg caacgatgat cgactggcgt
    37561 cctgttgttc atgaaatgcg cctgttcaaa tcgccagaag agattgccgt actccgccgc
    37621 gcgggagaaa tcaccgccat ggcacataca cgggcgatgg aaaaatgccg tccgggaatg
    37681 ttcgagtacc atctggaagg cgaaattcac cacgaattta accgccacgg tgcgcgctat
    37741 ccgtcctata acaccattgt cggcagcggt gaaaacggct gcattctgca ctacaccgaa
    37801 aacgagtgtg aaatgcgcga cggcgacctg gtgttgattg acgcgggttg tgaatacaaa
    37861 ggttacgctg gcgatattac ccgcaccttc ccggtcaacg gcaaattcac ccaggcccag
    37921 cgtgaaatct acgacattgt gctggagtct ctcgaaacca gcctgcgcct gtatcgtccg
    37981 ggaacttcca ttctggaagt cactggtgaa gtggtgcgca tcatggttag cggcctggta
    38041 aaactcggca tcctgaaagg tgatgttgat gaactgatcg ctcagaacgc ccatcgtcct
    38101 ttctttatgc atggccttag ccactggtta ggactggatg tccatgacgt gggtgtttat
    38161 ggtcaggatc gctcgcgcat tctggaaccg ggcatggtac tgaccgtaga gccagggctg
    38221 tatattgcgc cggatgcaga agtgccagaa caatatcgcg gtatcggcat tcgtattgaa
    38281 gacgacattg tgattaccga aaccggtaac gaaaacctca ccgccagcgt ggtgaaaaag
    38341 ccggaagaaa tcgaagcgtt gatggttgct gcgagaaagc aatgagcgta atcatcgtcg
    38401 gtggcggcat ggcgggcgcg acgctggcgc tggctatttc ccggttaagt cacggggcgc
    38461 tgccggtaca tttgattgaa gcgactgcgc cagagtcaca tgctcatccg ggctttgatg
    38521 gacgagcgat agcgctggcg gcgggtacct gtcagcaact ggcgcgcatc ggcgtctggc
    38581 aatctctggc ggattgcgca actgccatca ccaccgtgca tgtcagcgat cgtggtcacg
    38641 ctggatttgt caccctcgcc gcagaagatt accaactggc ggcgctggga caggttgtcg
    38701 aattgcacaa tgtcgggcaa cggctgtttg cattgctgcg taaagcacct ggcgtaacgc
    38761 tgcattgccc tgatcgcgtg gctaacgttg cccgtactca gagtcacgtt gaagtgacgc
    38821 tggagagtgg cgagacgctg acgggccgcg tgctggtagc agctgatggc acccattcag
    38881 cgttagccac cgcgtgcggc gttgactggc agcaggagcc ttacgaacaa ctggccgtga
    38941 ttgccaacgt tgctacttcc gttgcgcatg aagggcgcgc ttttgaacgc tttacgcaac
    39001 atggcccgct ggcgatgttg ccgatgtctg acggacgctg ttcgctggtc tggtgtcatc
    39061 cactggaacg gcgcgaagag gtgttgtcgt ggagtgacga gaagttttgc cgtgaactcc
    39121 agtcggcctt tggctggcga cttgggaaaa ttacccacgc tggtaaacgc agtgcttatc
    39181 cgctggcgtt aacccacgcc gccagatcta ttacccatcg taccgtgctg gtgggcaatg
    39241 cggcgcaaac tctgcacccg attgccgggc aagggtttaa cctcggtatg cgagatgtga
    39301 tgagtcttgc ggaaaccctg actcaggcgc aggagcgcgg agaagacatg ggggattacg
    39361 gcgtattgtg ccgttatcag cagcgtcgac agagcgatcg cgaagcaacc attggcgtca
    39421 cggacagcct tgtacatctt tttgccaacc gttgggcacc gctggttgtc gggcgcaaca
    39481 tcgggctgat gacgatggaa ttattcaccc cggcacgcga tgtgctggcg cagcgcaccc
    39541 tcggttgggt ggcgcgttga aggcggtttt aaggaataaa caatgcaaag tgttgatgta
    39601 gccattgttg gcggcggcat ggtggggctg gcggttgcct gtggcttaca ggggagcggc
    39661 ttacgcgttg ccgtactgga gcagcgcgta caggaacctc tggcggcgaa tgcaccacca
    39721 caactgcgcg tttcggctat caatgccgcc agcgaaaaat tactcacccg tcttggcgtc
    39781 tggcaggaca ttctctctcg tagggccagc tgttatcacg gtatggaagt gtgggacaaa
    39841 gacagctttg gtcacatttc gtttgacgat caaagcatgg gctatagcca tcttgggcat
    39901 atcgttgaaa attcagtgat tcactacgcg ctgtggaaca aagcgcatca gtcgtcagat
    39961 atcactctgt tagcccccgc agaattacag caggtcgcct ggggagaaaa tgaaaccttc
    40021 ctgacgctga aagatggcag catgttaacg gcgcgtctgg tgattggcgc ggacggcgct
    40081 aattcctggt tgcgcaacaa agccgatatt ccgctgactt tctgggatta tcagcatcac
    40141 gcgctggtag cgaccattcg cacggaagaa ccgcatgatg cggtggcgcg gcaggttttc
    40201 catggcgaag gcattctggc ctttttaccg cttagcgatc cgcatctttg ctcgattgtc
    40261 tggtcactgt cgccagagga agcgcagcgg atgcagcagg caagtgaaga cgaatttaat
    40321 cgcgcgttaa atatcgcttt tgataatcgc ctgggcttat gcaaggttga gagcgcgcgt
    40381 caggtgttcc cactgacggg gcgttatgcg cgccagtttg cctcgcaccg tctggcgctg
    40441 gtgggcgacg ccgcacatac cattcacccg ctggcggggc agggggtaaa tctcggcttt
    40501 atggatgctg cagagctgat tgccgaactg aaacggttgc atcgtcaggg gaaagacatc
    40561 gggcagtaca tttatctgcg tcgctatgag cgtagccgca agcacagtgc ggcgttgatg
    40621 ctggctggta tgcagggatt ccgcgatctg ttttccggta ccaatccggc gaaaaaactg
    40681 ctgcgtgata ttggtttgaa actggccgac acgcttcctg gcgttaagcc gcaacttatc
    40741 cgccaggcaa tgggattaaa cgatttgcct gaatggctgc gttaaaaatt tctcctctgt
    40801 tgtttatttg atacccatca cactttcatc tcccggtttt ttcgccggga gattttcctc
    40861 atttgaaata aactaatttc acctccgttt tcgcattata ttttctaatg ccattatttt
    40921 ttgatttagt gttttttgac atttttttag ctcttaatat tgtcttattc aaattgactt
    40981 tctcatcaca tcatctttgt atagaaactg gtgtattttt tggtttttta ttctgtcgcg
    41041 atttttgcat tttttaacca taagctaatg tgatgatcaa ttttacctta tggttaacag
    41101 tctgtttcgg tggtaagttc aggcaaaaga gaacgattgc gttggggacc gggagtggct
    41161 ccgatgctgg gtttcgtggt gataatttca ccatgaaaaa gttgtcagcc ccgcttattc
    41221 aatgaggaca agatggcaca acagactcct ttgtacgaac aacacacgct ttgcggcgct
    41281 cgcatggtgg atttccacgg ctggatgatg ccgctgcatt acggttcgca aatcgacgaa
    41341 catcatgcgg tacgtaccga tgccggaatg tttgatgtgt cacatatgac catcgtcgat
    41401 cttcgcggca gccgcacccg ggagtttctg cgttatctgc tggcgaacga tgtggcgaag
    41461 ctcaccaaaa gcggcaaagc cctttactcg gggatgttga atgcctctgg cggtgtgata
    41521 gatgacctca tcgtctacta ctttactgaa gatttcttcc gcctcgttgt taactccgcc
    41581 acccgcgaaa aagacctctc ctggattacc caacacgctg aacctttcgg catcgaaatt
    41641 accgttcgtg atgacctttc catgattgcc gtgcaagggc cgaatgcgca ggcaaaagct
    41701 gccacactgt ttaatgacgc ccagcgtcag gcggtggaag ggatgaaacc gttctttggc
    41761 gtgcaggcgg gcgatctgtt tattgccacc actggttata ccggtgaagc gggctatgaa
    41821 attgcgctgc ccaatgaaaa agcggccgat ttctggcgtg cgctggtgga agcgggtgtt
    41881 aagccatgtg gcttgggcgc gcgtgacacg ctgcgtctgg aagcgggcat gaatctttat
    41941 ggtcaggaga tggacgaaac catctctcct ttagccgcca acatgggctg gaccatcgcc
    42001 tgggaaccgg cagatcgtga ctttatcggt cgtgaagccc tggaagtgca gcgtgagcat
    42061 ggtacagaaa aactggttgg tctggtgatg accgaaaaag gcgtgctgcg taatgaactg
    42121 ccggtacgct ttaccgatgc gcagggcaac cagcatgaag gcattatcac cagcggtact
    42181 ttctccccga cgctgggtta cagcattgcg ctggcgcgcg tgccggaagg tattggcgaa
    42241 acggcgattg tgcaaattcg caaccgtgaa atgccggtta aagtgacaaa acctgttttt
    42301 gtgcgtaacg gcaaagccgt cgcgtgattt acttttttgg agattgattg atgagcaacg
    42361 taccagcaga actgaaatac agcaaagaac acgaatggct gcgtaaagaa gccgacggca
    42421 cttacaccgt tggtattacc gaacatgctc aggagctgtt aggcgatatg gtgtttgttg
    42481 acctgccgga agtgggcgca acggttagcg cgggcgatga ctgcgcggtt gccgaatcgg
    42541 taaaagcggc gtcagacatt tatgcgccag taagcggtga aatcgtggcg gtaaacgacg
    42601 cactgagcga ttccccggaa ctggtgaaca gcgaaccgta tgcaggcggc tggatcttta
    42661 aaatcaaagc cagcgatgaa agcgaactgg aatcactgct ggatgcgacc gcatacgaag
    42721 cattgttaga agacgagtaa cggctttatt cctcttctgc gggagaggat cagggtgagg
    42781 aaaatttatg cctcaccctc actctcttcg taaggagaga ggttcacaat tcactgcacg
    42841 tttcaggaac catcgctcat gacacagacg ttaagccagc ttgaaaacag cggcgctttt
    42901 attgaacgcc atatcggacc ggacgccgcg caacagcaag aaatgctgaa tgccgttggt
    42961 gcacaatcgt taaacgcgct gaccggccag attgtgccga aagatattca acttgcgaca
    43021 ccaccgcagg ttggcgcacc ggcgaccgaa tacgccgcac tggcagaact caaggctatt
    43081 gccagtcgca ataaacgctt cacgtcttac atcggcatgg gttacaccgc cgtgcagcta
    43141 ccgccggtta tcctgcgtaa catgctggaa aatccgggct ggtataccgc gtacactccg
    43201 tatcaacctg aagtctccca gggccgcctt gaagcactgc tcaacttcca gcaggtaacg
    43261 ctggatttga ctggactgga tatggcctct gcttctcttc tggacgaggc caccgctgcc
    43321 gccgaagcaa tggcgatggc gaaacgcgtc agcaaactga aaaatgccaa ccgcttcttc
    43381 gtggcttccg atgtgcatcc gcaaacgctg gatgtggtcc gtactcgtgc cgaaaccttt
    43441 ggttttgaag tgattgtcga tgacgcgcaa aaagtgctcg accatcagga cgtcttcggc
    43501 gtgctgttac agcaggtagg cactaccggt gaaattcacg actacactgc gcttattagc
    43561 gaactgaaat cacgcaaaat tgtggtcagc gttgccgccg atattatggc gctggtgctg
    43621 ttaactgcgc cgggtaaaca gggcgcggat attgtttttg gttcggcgca acgcttcggc
    43681 gtgccgatgg gctacggtgg cccacacgcg gcattctttg cggcgaaaga tgaatacaaa
    43741 cgctcaatgc cgggccgtat tatcggtgta tcgaaagatg cagctggcaa taccgcgctg
    43801 cgcatggcga tgcagactcg cgagcaacat atccgccgtg agaaagcgaa ctccaacatt
    43861 tgtacttccc aggtactgct ggcaaacatc gccagcctgt atgccgttta tcacggcccg
    43921 gttggcctga aacgtatcgc taaccgcatt caccgtctga ccgatatcct ggcggcgggc
    43981 ctgcaacaaa aaggtctgaa actgcgccat gcgcactatt tcgacacctt gtgtgtggaa
    44041 gtggccgaca aagcgggcgt actgacgcgt gccgaagcgg ctgaaatcaa cctgcgtagc
    44101 gatattctga acgcggttgg gatcaccctt gatgaaacaa ccacgcgtga aaacgtaatg
    44161 cagcttttca acgtgctgct gggcgataac cacggcctgg acatcgacac gctggacaaa
    44221 gacgtggctc acgacagccg ctctatccag cctgcgatgc tgcgcgacga cgaaatcctc
    44281 acccatccgg tgtttaatcg ctaccacagc gaaaccgaaa tgatgcgcta tatgcactcg
    44341 ctggagcgta aagatctggc gctgaatcag gcgatgatcc cgctgggttc ctgcaccatg
    44401 aaactgaacg ccgccgccga gatgatccca atcacctggc cggaatttgc cgaactgcac
    44461 ccgttctgcc cgccggagca ggccgaaggt tatcagcaga tgattgcgca gctggctgac
    44521 tggctggtga aactgaccgg ttacgacgcc gtttgtatgc agccgaactc tggcgcacag
    44581 ggcgaatacg cgggcctgct ggcgattcgt cattatcatg aaagccgcaa cgaagggcat
    44641 cgcgatatct gcctgatccc ggcttctgcg cacggaacta accccgcttc tgcacatatg
    44701 gcaggaatgc aggtggtggt tgtggcgtgt gataaaaacg gcaacatcga tctgactgat
    44761 ctgcgcgcga aagcggaaca ggcgggcgat aacctctcct gtatcatggt gacttatcct
    44821 tctacccacg gcgtgtatga agaaacgatc cgtgaagtgt gtgaagtcgt gcatcagttc
    44881 ggcggtcagg tttaccttga tggcgcgaac atgaacgccc aggttggcat cacctcgccg
    44941 ggctttattg gtgcggacgt ttcacacctt aacctacata aaactttctg cattccgcac
    45001 ggcggtggtg gtccgggtat gggaccgatc ggcgtgaaag cgcatttggc accgtttgta
    45061 ccgggtcata gcgtggtgca aatcgaaggc atgttaaccc gtcagggcgc ggtttctgcg
    45121 gcaccgttcg gtagcgcctc tatcctgcca atcagctgga tgtacatccg catgatgggc
    45181 gcagaagggc tgaaaaaagc aagccaggtg gcaatcctca acgccaacta tattgccagc
    45241 cgcctgcagg atgccttccc ggtgctgtat accggtcgcg acggtcgcgt ggcgcacgaa
    45301 tgtattctcg atattcgccc gctgaaagaa gaaaccggca tcagcgagct ggatattgcc
    45361 aagcgcctga tcgactacgg tttccacgcg ccgacgatgt cgttcccggt ggcgggtacg
    45421 ctgatggttg aaccgactga atctgaaagc aaagtggaac tggatcgctt tatcgacgcg
    45481 atgctggcta tccgcgcaga aattgaccag gtgaaagccg gtgtctggcc gctggaagat
    45541 aacccgctgg tgaacgcgcc gcacattcag agcgaactgg tcgccgagtg ggcgcatccg
    45601 tacagccgtg aagttgcggt attcccggca ggtgtggcag acaaatactg gccgacagtg
    45661 aaacgtctgg atgatgttta cggcgaccgt aacctgttct gctcctgcgt accgattagc
    45721 gaataccagt aattcactga ttcgactatt ttctaaaggc gcttcggcgc ctttttagtc
    45781 agatgacaaa gtacaaaagt gctcagacag tcccctcgcc cctttgggga gagggttagg
    45841 gtgaggggaa caggccggca ctggcgcgaa tatttaccct caccccggcc ctctccctga
    45901 aagggcgagg gggaaaagcg tgccaacatt gaagattgag ccagtttgtt agcaatctca
    45961 aagatacgtc aacgaattaa tttttctcgg aaaaacaaat ggctatagca cttgtgactg
    46021 gtggcagtcg cggcatcggg cgggcaactg cattactgtt ggcgcaagaa gggtatacgg
    46081 tggcggttaa ttatcagcaa aacctccacg cggcgcagga agtgatgaac ttaataacgc
    46141 aagccggtgg caaagcattc gtgctccagg cggatatcag cgacgaaaac caggtcgttg
    46201 cgatgtttac agcaatcgat cagcacgatg aaccgctagc agcgctggtc aataacgccg
    46261 ggatcttgtt tacccagtgc accgttgaaa accttaccgc agagcgaatc aaccgagtac
    46321 tttccaccaa cgtgacggga tattttctct gctgccgcga ggcggtaaaa cgcatggcgc
    46381 ttaaaaatgg tggcagtggc ggcgctatcg tcaatgtctc ttcggtggcc tcacggttgg
    46441 gttcgccagg ggaatatgtt gattacgcgg catcgaaagg ggcgattgat acgttaacca
    46501 ccggactatc gctggaagtc gccgcgcagg ggatccgcgt taactgcgtg cggccagggt
    46561 ttatttatac cgaaatgcac gccagcggcg gcgagcctgg acgcgtcgat cgcgttaagt
    46621 cgaacatccc catgcagcgt ggtggacagg cagaagaggt cgcgcaggcc attgtctggc
    46681 tactaagtga taaagcctct tacgtcacgg gaagttttat cgatttggcg ggcgggaaat
    46741 aaaacaggga agttgtctga ccggatgcaa caagtattgc atccggtact tcatcgactt
    46801 aaagcttctc gccgttgctg gcaatcacct ctttgtacca gttaaagctc ttcttacgtg
    46861 aacgcgacat atcgccagta ccgtcgtcat gtttattcac atagataaag ccgtagcgtt
    46921 tgctgtactg cccggtggtg aacgacacgc agtcgatgca gccccacggt gtgtagccca
    46981 tcagatccac gccatcgtaa gtcaccgctt ttttcatctc ttcgatatgg gcgcgcaggt
    47041 agtcaatgcg gtagtcgtcg ttgatgctgc catcttcttc cactttgtcg taagcgccaa
    47101 aaccgttttc gacaataaac agcggcctct gataacgctc atacagttcg caaagtgcat
    47161 agcgcagacc tactggatca atctgccagc cccagtcaga tgctttaaca tacgggtttg
    47221 gtacgctgcc ttcaaaacca gagatcgcat cgccggtgcc gccttcggcc ttcactgcat
    47281 tggtcatgta atagctgaaa ccaagataat cgcaggtgcc ttcacgcagc acatccagat
    47341 cgccgtcttc cattttgatg ttaaatccgc gacgctccca ctcgttcaac acataggacg
    47401 ggtaatagcc gcgtagctgc acatcggtaa agacgtagcg ttcgcgcatc gactcctgag
    47461 cgaacatcac atcgtccggg ttacaggagt aaggatagag cggcaccatc gccagcatac
    47521 agccgacttt catctccggg ttaatgcgac gcgcagcttt caccgccagg gcgctggcga
    47581 caaactggtg atgcagcacc tgatacatcg tctcttccgg gttttcatgc tcggtataca
    47641 ccacgccgga gcagcagtaa ccgaacagcg gtgcacgcca gttacgctgg ttgttaattt
    47701 cgttgaaggt catccagtat ttgactttgt gcttatagcg ttcaaatacc acttccgcga
    47761 aacgtacaaa gaaatcaacc actttacggt tggtccagct accgtattgc tgcaccagat
    47821 gcagcggcat ttcaaagtgg gagagggtga tcaccggttc gatgttgtat ttcagcagtt
    47881 catcgaacat atcatcgtag aacttcagcc cttcttcgtt tggctgagct tcatcgcctt
    47941 ttggaaaaat gcgcgtccag gcaatggatg tacgaaaaca tttgaagccc atttcggcaa
    48001 atagcttgat gtcttcctta tagtgaccat aaaaatcaac ggcttcatgg tttggatagt
    48061 attttcctgg caagacttct ttggtgattt cgcgcggcac gccgtgtgcg ccaccggtca
    48121 gaacgtcaca aatgctcggc ccttttccgc ctttgttcca gccgccttcg acctgatgag
    48181 cggcaactgc gccgccccat aagaaatctt tcggtaaggt gagttttttc actatcatag
    48241 gctccagatg agtcaattct tacaggaaat agtaccatgc agccaaacga catcactttt
    48301 tttcaacgtt tccaggatga cattctggct gggcgtaaaa ccatcaccat ccgcgacgag
    48361 tctgaatcgc acttcaaaac gggtgatgtg cttcgtgtcg gacgttttga agatgacggt
    48421 tatttttgca cgattgaagt caccgcaacc tcaaccgtaa cgctggatac gctgactgaa
    48481 aaacatgcag agcaggaaaa tatgaccctg actgaactga aaaaagtcat tgctgacatc
    48541 tatcccggcc agacacaatt ttatgtgatt gaatttaaat gtctttaaat tgaactcacg
    48601 cctgttagtt gaaatttcgc atttaatttc atgattttta ggagattagt ctcaaatcag
    48661 aatttatttt agctaacagg tgttcactgg aactattctc agttacgctg gagaggtata
    48721 catgtgcgtg tacgtttctc cggagtaagt tatggttcag aagcccctca ttaagcaggg
    48781 atattcactg gcagaggaaa tagccaacag cgtcagtcac ggcattgggt tggtgtttgg
    48841 tatcgttggg ctggtgttgc tactggttca ggcggtggat cttaatgcca gcgccacagc
    48901 gataaccagc tacagcctct atggcggcag tatgatcctg ctgttcctcg cttcgacgct
    48961 ctatcacgcc attcctcatc aacgggcaaa aatgtggctg aagaaatttg accattgcgc
    49021 tatttacctg ttgattgccg gaacctacac gccgtttttg ctggtggggc tggattctcc
    49081 gttagcgcgc gggttgatga ttgttatctg gaacctggca ttgctgggta ttctgtttaa
    49141 actgaccatc gcgcaccgat tcaaaatttt atctctggtg acctatctgg cgatgggctg
    49201 gctgtcgctg gtggtaattt atgaaatggc agttaagctc gcggcgggca gcgttacctt
    49261 actggcggta ggcggcgtgg tttattcgct cggggtgatt ttctacgtct gcaaacgcat
    49321 tccatacaac catgccatct ggcacggctt cgtgctcggc ggtagtgtgt gccactttct
    49381 ggcgatctat ttgtatattg ggcaggcgta atgggtatgc ctttgtaggc ctgataagac
    49441 gcgccagcgt cgcatcaggc atcggagcac aactgccgga ttcggcgtga acgccttatc
    49501 cgatctacgg gcctcgtccc tgttgtgata aaggcatcaa atcccagaca tgataagacg
    49561 cgccagcgtc gcatcaggcg ataaatgcct gatgcgataa agagatttac tcttcgagcg
    49621 aatacggcag cggctcgata tgcaatgtat tcgcatcgtc gcgtacgcgg aagatgctat
    49681 ccggttccat atcgttattc atgacgacct gtaccacgac ctgaccatct tccagtttta
    49741 ccgcagccag taccgtaccg gtacgacgcc agttctcgcc cattttcagc tctaagtctt
    49801 caccagcttc cggcagtcgg ctggcgctac ctgccagcaa ccagagcgca cgtttattgg
    49861 caccacggaa ttttgctcgc gccaccatct cttgtccggt ataacagcct ttcttaaagc
    49921 tgataccgcc cagcgcctgg agattggtcg cctgtgggat aaactgcccg ctgttggcgg
    49981 catcaatcac cgggaaaccc gcttcaatgt ttaatgccag ccactgttgg ctattgttca
    50041 gttccgcttc accgcgcagt ttatcggtca gcatattagc agtagcttca tcggttacga
    50101 tcaggaaacg ttctgccggg tgttcaaacc atagcaaagt ggtcgcgcct tctttgacta
    50161 cctgtttttc tttcgaaggc agttcgctaa agagatttgc cagcgcggcg cgcgcctgaa
    50221 aaccggcaac accaagcagc acacgctcgt cgtctggcgc gatggtcact ttagagaata
    50281 ccgcatattt tttcagttca gtcagctgcg gttcacgcac gctgcgccgt tcaatccatg
    50341 caaagccatc gccgtcgcgg aacagacgta aattgctcca cattttacct ttggcgtcgc
    50401 aatgggcggc gagcaggtgc tgatcttctg ccatctggct gacatctgct gtcacctgac
    50461 cctgcatata tttttcgctg tccgcgccag taatggtggc aagcgcccag tcatcaagcg
    50521 tcatcagcgt cagcggtaaa cgggcagaag ccgtaggctg acggggagga aaaggtgtaa
    50581 aagccataat agagtcctga gttgcttaac ggcgtattaa tgactaatgg taaaagagct
    50641 atgaggcaat gcaagtgctt tcactacgcg atctgtgcct tctgcgaggc gacttccaaa
    50701 aaaatgaagc cattgaattg ttgtgaatta atttcgcgat cgcgatggtg tttactgata
    50761 ttcccgtaaa agaaggcgaa aaacacgtta caatagccgg gtactggatt ttcgtgagac
    50821 aggaagaaga tggacattaa caacaaagcc cgcattcatt gggcatgccg ccgtggtatg
    50881 cgcgaactcg atatttcaat catgccgttt ttcgaacatg agtacgacag cttaagcgat
    50941 gacgaaaaac gcatctttat tcgtctgctg gaatgtgacg atccggacct gtttaactgg
    51001 ctgatgaatc acggtaaacc ggccgatgca gaactggaaa tgatggtccg actcatccag
    51061 acacggaacc gggaacgtgg tcctgtggca atctgatttg cgcgtctcct ggcgcgcaca
    51121 gtggctttcc ttgctgattc atgggctggt tgccgctgtt attttactca tgccctggcc
    51181 actcagttac accccgttat ggatggtgtt actttcgctg gtggtgtttg attgcgttcg
    51241 cagccagcgg cgtattaatg ctcgccaggg ggaaattcgc ttgttgatgg acgggcgttt
    51301 gcgttggcaa gggcaggagt ggagcatcgt caaagcaccg tggatgatta agagcggcat
    51361 gatgctgcgt ttacgttctg atggcggtaa acggcaacat ttatggctgg cagccgacag
    51421 catggacgaa gctgaatggc gggatttacg gcggattttg ttgcaacaag agacgcaaag
    51481 ataaagatga cggcaaagca tatgcgctaa gccgtcagtg agatcaggcg taatgctctg
    51541 ccatttcgtt gaggatttgc tcgcaccagc tctgaatacg ctcgtcgcta aggtcatact
    51601 ggttagtttc atccagcgcc agacccacga acagttgccc gtcagcaatc accggtttcg
    51661 ggctggtaaa ttcatatcct tccgttggcc agtagccgac gaacttcacg cctttggtcg
    51721 agagtttgtc atgcagcata ccgagcgcat cgaggaacca ctcgccgtat cccagttgat
    51781 cgccaagccc atacagcgca acaattttac cttcaaggtt caggtcgtcg agctgatccc
    51841 agacggcttc ccagtcttcc tggatttcac caaaatccca ggtcgggata cccagaatga
    51901 gcacatcgta ctgctccatt aatttcgggg agtcgtcctt gaggttatgt aaggtcacca
    51961 gttctgggcc gataatatcg cggatttttt ctgccgccat ttcggtgtaa caggtgctgg
    52021 aaccgtaaaa aagacccata ttcataacgt aagcttctcg attctgctgt ggcttttgcg
    52081 gctagtgtat cagaatcgct ttagtcacgg cacaataagg cataatggat caaatgagtg
    52141 accataaggg gcgcaagtga aacaggatct ggcacgcatc gagcagtttc ttgatgctct
    52201 gtggctggaa aaaaatctgg ctgaaaatac gttgaacgct taccgtcgcg atctgtcaat
    52261 gatggtggag tggttgcatc accgcgggtt gacgctggcg acggcgcaaa gtgacgattt
    52321 gcaggcatta ctggcagaac ggctggaggg cgggtataaa gccaccagct cagcgcgttt
    52381 gctgagtgca gtgcgccgat tgttccagta tctttatcgc gaaaagtttc gtgaagacga
    52441 tcccagtgcg catctcgctt caccgaaatt gccccagcgt ttgccaaaag atttaagcga
    52501 agcgcaggtc gaacgtttat tacaggcacc attaatcgat cagccactgg agctacgcga
    52561 taaagccatg cttgaagtgt tgtatgctac cgggctgcgt gtctctgaac tggtcggact
    52621 gacaatgagt gatatcagcc tgcgtcaggg cgtggtacgg gtcattggta aaggcaacaa
    52681 agagcgtctg gtgccgttag gtgaagaggc ggtttactgg ctggaaacct atctggaaca
    52741 tgggcgtccg tggctgctga atggtgtgtc aattgacgtg ttgtttccca gccagcgtgc
    52801 gcagcagatg acgcgacaga ccttctggca ccgtattaaa cattatgctg tgctggcggg
    52861 tatcgacagc gaaaagctgt caccgcatgt gttgcgtcac gcttttgcca ctcatttatt
    52921 aaatcatggt gcggatttac gcgtggtgca gatgctactg ggccacagcg atctctccac
    52981 cacgcaaatt tatacgcatg tcgctaccga gcgtctgcgg caacttcatc aacagcatca
    53041 cccgcgggcg tgatgtctga aaagaacggg aagatttatg aagaaaggtt ttatgttgtt
    53101 tactttgtta gcggcgtttt caggctttgc tcaggctgat gacgcggcaa ttcaacaaac
    53161 gttagccaaa atgggcatca aaagcagcga tattcagccc gcgcctgtag ctggcatgaa
    53221 gacagttctg actaacagcg gcgtgttgta catcaccgat gatggtaaac atatcattca
    53281 ggggccaatg tatgacgtta gtggcacggc tccggtcaat gtcaccaata agatgctgtt
    53341 aaagcagttg aatgcgcttg aaaaagagat gatcgtttat aaagcgccgc aggaaaaaca
    53401 cgtcatcacc gtgtttactg atattacctg tggttactgc cacaaactgc atgagcaaat
    53461 ggcagactac aacgcgctgg ggatcaccgt gcgttatctt gctttcccgc gccaggggct
    53521 ggacagcgat gcagagaaag aaatgaaagc tatctggtgt gcgaaagata aaaacaaagc
    53581 gtttgatgat gtgatggcag gtaaaagcgt cgcaccagcc agttgcgacg tggatattgc
    53641 cgaccattac gcacttggcg tccagcttgg cgttagcggt actccggcag ttgtgctgag
    53701 caatggcaca cttgttccgg gttaccagcc gccgaaagag atgaaagaat tcctcgacga
    53761 acaccaaaaa atgaccagcg gtaaataatt cgcgtgaaac aacagataca acttcgtcgc
    53821 cgtgaagtcg atgaaacggc agacttgccc gctgaattgc ctcccttgct gcgccgttta
    53881 tacgccagcc ggggagtacg cagtgcgcaa gaactggaac gcagtgttaa aggtatgctg
    53941 ccctggcagc aactgagcgg cgtcgaaaag gccgttgaga tcctttacaa cgcttttcgc
    54001 gaaggaacgc ggattattgt ggtcggtgat ttcgacgccg acggcgcgac cagcacggct
    54061 ctaagcgtgc tggcgatgcg ctcgcttggt tgcagcaata tcgactacct ggtaccaaac
    54121 cgtttcgaag acggttacgg cttaagcccg gaagtggtcg atcaggccca tgcccgtggc
    54181 gcgcagttaa ttgtcacggt ggataacggt atttcctccc atgcgggggt tgagcacgct
    54241 cgctcgttgg gcatcccggt tattgttacc gatcaccatt tgccaggcga cacattaccc
    54301 gcagcggaag cgatcattaa ccctaacttg cgcgactgta atttcccgtc gaaatcactg
    54361 gcaggcgtgg gtgtggcgtt ttatctgatg ctggcgctgc gcaccttttt gcgcgatcag
    54421 ggctggtttg atgagcgtaa catcgcaatt cctaacctgg cagaactgct ggatctggtc
    54481 gcgctgggga cagtggcgga cgtcgtgccg ctggacgcta ataatcgcat tctgacctgg
    54541 caggggatga gtcgcatccg agccggaaag tgccgtccgg ggattaaagc gctgcttgaa
    54601 gtggcaaacc gtgatgcaca aaaactcgcc gccagcgatt taggttttgc gctggggcca
    54661 cgtctcaatg ctgccggacg actggacgat atgtccgtcg gtgtggcgct gttgttgtgc
    54721 gacaacatcg gcgaagcgcg cgtgctggca aatgaactcg atgcgctaaa ccagacgcga
    54781 aaagagatcg aacaaggaat gcaaattgaa gccctgaccc tgtgcgagaa actggagcgc
    54841 agccgtgaca cgctacccgg cgggctggca atgtatcacc ccgaatggca tcagggcgtt
    54901 gtcggtattc tggcttcgcg catcaaagag cgttttcacc gtccggttat cgcgtttgcg
    54961 ccagcaggtg acggtacgct gaaaggttcc ggtcgctcca ttcaggggct gcatatgcgt
    55021 gatgcgctgg agcgattaga cacactctac cctggcatga tgctgaagtt tggcggtcat
    55081 gcgatggcgg cgggtttgtc gctggaagag gataaattca aactctttca acaacggttt
    55141 ggcgaactgg ttactgagtg gctggaccct tcgctattgc aaggcgaagt ggtatcagac
    55201 ggtccgttaa gcccggccga aatgaccatg gaagtggcgc agctgctgcg cgatgctggc
    55261 ccgtgggggc agatgttccc ggagccgctg tttgacggtc atttccgtct gctgcaacag
    55321 cggctggtgg gcgaacgtca tttgaaggtg atggtcgaac cggtcggcgg cggtccactg
    55381 ctggatggta ttgcttttaa tgtcgatacc gccctctggc cggataacgg cgtgcgcgaa
    55441 gtgcaactgg cttataagct cgatatcaac gagtttcgcg gcaaccgcag cctgcaaatt
    55501 atcatcgaca atatctggcc aatttagcgt catcttctct ataaaaaaga gcgtggattg
    55561 ggtacaatcc cgctcttatc accgcatttt gactagctca ataaaagaaa tcagaccatg
    55621 tttgaaatta atccggtaaa taatcgcatt caggacctca cggaacgctc cgacgttctt
    55681 agggggtatc tttgactacg acgccaagaa agagcgtctg gaagaagtaa acgccgagct
    55741 ggaacagccg gatgtctgga acgaacccga acgcgcacag gcgctgggta aagagcgttc
    55801 ctccctcgaa gccgttgtcg acaccctcga ccaaatgaaa caggggctgg aagatgtttc
    55861 tggtctgctg gaactggctg tagaagctga cgacgaagaa acctttaacg aagccgttgc
    55921 tgaactcgac gccctggaag aaaaactggc gcagcttgag ttccgccgta tgttctctgg
    55981 cgaatatgac agcgccgact gctacctcga tattcaggcg gggtctggcg gtacggaagc
    56041 acaggactgg gcgagcatgc ttgagcgtat gtatctgcgc tgggcagaat cgcgtggttt
    56101 caaaactgaa atcatcgaag agtcggaagg tgaagtggcg ggtattaaat ccgtgacgat
    56161 caaaatctcc ggcgattacg cttacggctg gctgcgtaca gaaaccggcg ttcaccgcct
    56221 ggtgcgtaaa agcccgtttg actccggcgg tcgtcgccac acgtcgttca gctccgcgtt
    56281 tgtttatccg gaagttgatg atgatattga tatcgaaatc aacccggcgg atctgcgcat
    56341 tgacgtttat cgcgcgtccg gcgcgggcgg tcagcacgtt aaccgtaccg aatctgcggt
    56401 gcgtattacc cacatcccga ccgggatcgt gacccagtgc cagaacgacc gttcccagca
    56461 caagaacaaa gatcaggcca tgaagcagat gaaagcgaag ctttatgaac tggagatgca
    56521 gaagaaaaat gccgagaaac aggcgatgga agataacaaa tccgacatcg gctggggcag
    56581 ccagattcgt tcttatgtcc ttgatgactc ccgcattaaa gatctgcgca ccggggtaga
    56641 aacccgcaac acgcaggccg tgctggacgg cagcctggat caatttatcg aagcaagttt
    56701 gaaagcaggg ttatgaggaa ccaacatgtc tgaacaacac gcacagggcg ctgacgcggt
    56761 agtcgatctt aacaatgaac tgaaaacgcg tcgtgagaag ctggcgaacc tgcgcgagca
    56821 ggggattgcc ttcccgaacg atttccgtcg cgatcatacc tctgaccaat tgcacgcaga
    56881 attcgacggc aaagagaacg aagaactgga agcgctgaac atcgaagtcg ccgttgctgg
    56941 ccgcatgatg acccgtcgta ttatgggtaa agcgtctttc gttaccctgc aggacgttgg
    57001 cggtcgcatt cagctgtacg ttgcccgtga cgatctcccg gaaggcgttt ataacgagca
    57061 gttcaaaaaa tgggacctcg gcgacatcct cggcgcgaaa ggtaagctgt tcaaaaccaa
    57121 aaccggcgaa ctgtctatcc actgcaccga gttgcgtctg ctgaccaaag cactgcgtcc
    57181 gctgccggat aaattccacg gcttgcagga tcaggaagcg cgctatcgtc agcgttatct
    57241 cgatctcatc tccaacgatg aatcccgcaa cacctttaaa gtgcgctcgc agatcctctc
    57301 tggtattcgc cagttcatgg tgaaccgcgg ctttatggaa gttgaaacgc cgatgatgca
    57361 ggtgatccct ggcggtgccg ctgcgcgtcc gtttatcacc caccataacg cgctggatct
    57421 cgacatgtac ctgcgtatcg cgccggaact gtacctcaag cgtctggtgg ttggtggctt
    57481 cgagcgtgta ttcgaaatca accgtaactt ccgtaacgaa ggtatttccg tacgtcataa
    57541 cccagagttc accatgatgg aactctacat ggcttacgca gattacaaag atctgatcga
    57601 gctgaccgaa tcgctgttcc gtactctggc acaggatatt ctcggtaaga cggaagtgac
    57661 ctacggcgac gtgacgctgg acttcggtaa accgttcgaa aaactgacca tgcgtgaagc
    57721 gatcaagaaa tatcgcccgg aaaccgacat ggcggatctg gacaacttcg actctgcgaa
    57781 agcaattgct gaatctatcg gcatccacgt tgagaagagc tggggtctgg gccgtatcgt
    57841 taccgagatc ttcgaagaag tggcagaagc acatctgatt cagccgacct tcattactga
    57901 atatccggca gaagtttctc cgctggcgcg tcgtaacgac gttaacccgg aaatcacaga
    57961 ccgctttgag ttcttcattg gtggtcgtga aatcggtaac ggctttagcg agctgaatga
    58021 cgcggaagat caggcgcaac gcttcctgga tcaggttgcc gcgaaagacg caggtgacga
    58081 cgaagcgatg ttctacgatg aagattacgt caccgcactg gaacatggct taccgccgac
    58141 agcaggtctg ggaattggta tcgaccgtat ggtaatgctg ttcaccaaca gccataccat
    58201 ccgcgacgtt attctgttcc cggcgatgcg tccggtaaaa taagcattac gttatgctca
    58261 caacctcggc aaatgtcggg gtttttttat ttaagctggg taaatgcaga taatcgtttt
    58321 ctggcttcgc gatttgtcgc ctgcatcacc atccacggac tgaacgccca cggcgtggca
    58381 tcaataccgt gtaatacatc tgctaaatca caccattgat aatccatcac ttcatcatca
    58441 ttgatctgta acgcactagt ggtgcgtgcg gcaaataccg gacacacttc attttccaca
    58501 atgccactcg gatcggtggc gcggtagcga aagtcaggat agatagattc aggaggcgta
    58561 atttccacgc caagctcata acggcaacgg cggatcactg cgtcttcgtt gctttctccc
    58621 agttgtgggt gcccacaaac cgagttagtc cacacgccag gccatgcttt tttgctcagt
    58681 gcgcggcggg taactaataa ttgtcctttg gcattaaaca gccaactgga gaacgcgaga
    58741 tgtaagcggg tgtctgccgt gtgtgcggca tacttttcca gcgtacccgt gggaactccc
    58801 tgtgcattca ataaaatgac gtgttccgtt tgcataattt ctcacatgta attctgatca
    58861 ctcatcatta tagataattg aagagtgaaa tgattaacca agtgagcgtt tatcgacaac
    58921 cgcccgtttt gagcggatgc cgacaggtaa aaaccattta aaaaagtagc atcatatatt
    58981 tgaagcgact ttcaatggcc attgcaacac agtcagaaat gacaggaata gagagagtaa
    59041 aaagcaacaa cagactgaat atagcgcgca ttaaaaaatt catattattc tccatgctca
    59101 tttttcttca gcttgcgcat cagaagccac atacgtactg tcctgacttt taaatcttta
    59161 tcagagacgg attccttcac caggtcagca tgatgctgat agccattaaa gaagacgttc
    59221 agcacaacgg cgctaagggt tgcgagcata atgccgctat gtagcagcgg ttgcagtacg
    59281 gccggtaact tagaaaagaa atcgtgagag agcgtcggag tcatgccaac gccgagactg
    59341 atcgccacaa tatagaggtt gtaacggttg gtggtgtagt tacagcgcga cagaattcga
    59401 atccctgtcg ccagtaccat gccgaacatc actagaccag cgccgcccag cacaaattgc
    59461 ggaatggagg ctaccagcac cgccattttt ggcaccatgc caaacaggat taatataatt
    59521 cccgaagaaa tacacaccca gcggctatga acgcgcgtca cgctaaccag gccaacgttt
    59581 tgagaaaatg acgtgtgggg gaagctgtta aacgtgccgc ctatcattgt ccctacgcca
    59641 tcgacacgca gcccgcgaat aatatcgtgc gaagagagtt tacgaccgac tatttcaccc
    59701 agtgccagga acatccccat cgactcgata aacacgatga ttaacacggc agtcatggtc
    59761 agaatggaaa cgggatcgaa aatcggcata ccaaacgaca tcggcgtaac aatcgcaaac
    59821 catgaagcat catgtagccc ggataaattg acttcattca tcatccacga aagtaaaaag
    59881 ccaaatacaa tccccagtaa tacggcgacg ttggacataa atcctttcgc atagcgagta
    59941 atgagcaaga taaaaattaa gacggcaaag gagataccta aataaacggg attaccatat
    60001 tgcggattcc ctttacctcc ggcggcccag tcaataccca cctgaatgat gctaagcccg
    60061 atagaagtaa taaccacacc ggtaaccagt ggcgggaata aaggcatcaa gcgaccgata
    60121 agtggcgcta ataatgtggt gataaaaccc gcggcgatag tggcaccaaa tatccccagc
    60181 aggccgatat ccgggttcat cccaatggct atcattggtg ttacagcagc aaaggtcacc
    60241 gacataatca ccggcaggcg gatccccata aagcggccga taccgataca ttgcaataat
    60301 gtgacgatcc cgcagcaaaa gagatccgag ctaatgagca tcgcaatagc ttcttttgag
    60361 aggcccagtc ggtcaccaat cataagagga acagcgactg cacctgcgta catgacaagg
    60421 acgtgttgca gaccgagtat gatcagcttt cctggtgata atatgcggtc aacctcatca
    60481 gttgggcggt cttgcccaga agatgaggga agttgggaat ctatggcgct catcagtgtt
    60541 tctcctttcg cccgccagag taaaaagcaa ccgtttacgt taatgaaact gtctaaacac
    60601 atgctcgtgg agtgccagtt tttgcgagga aggggagtta gagacaggaa ttgcggaggc
    60661 aatcacataa tcgtggttaa accacgaaaa tgagaagaga acctgacaat ggaattatct
    60721 gtaattttct catattgata tttcattaat cattctggca tgtcgcactc tcgcatttaa
    60781 tcgtttttat ctggatagcg ctcttttgat cggcaatgtt gaaataatga aagcggcata
    60841 gattgagggg caataatttg aactcagtcg caaaattata attaaaacgg tcatgctacc
    60901 taatatatgc ccgtttattg catcgcgaaa tttgagaaga cataactcat taaatgaaag
    60961 ggatgcaaat gaataagttt attgctgctg aagctgcgga atgtataggc tgtcatgctt
    61021 gtgaaattgc ctgtgcggtg gcacacaatc aagaaaactg gccgctgagt cacagtgact
    61081 ttcgaccgcg tatccacgtt gtagggaaag gccaggctgc gaatccggtg gcctgccatc
    61141 actgcaacaa tgccccttgc gttacggctt gtccggttaa tgctctgact ttccagtccg
    61201 atagcgtaca actggacgag caaaaatgta ttggttgtaa aagatgcgca atcgcttgcc
    61261 cctttggcgt cgttgagatg gtcgatacga ttgcgcagaa atgcgacctt tgtaaccagc
    61321 gcagttccgg cacgcaagcc tgtattgaag tttgcccaac acaggcgtta cgactgatgg
    61381 acgataaagg gttgcagcag ataaaggtgg cccgccagcg caaaacggca gcaggaaaag
    61441 cgtcatcaga cgctcagcca tctcgcagtg cagcgttgct ccccgttaac tcgcgtaaag
    61501 gcgcagataa aatttcagcg agtgaacgga aaacccactt tggcgaaata tattgcgggc
    61561 tggatccaca acaagcgact tatgagagtg accgctgtgt ttattgtgcc gaaaaagcta
    61621 actgcaactg gcattgcccg ctgcataacg ctattccgga ttacatccgt ctggtacagg
    61681 aaggaaagat tattgaagcg gcagaacttt gccaccagac cagttcctta ccagaaattt
    61741 gcggcagggt atgtccacag gaccgtcttt gtgaaggcgc atgtactttg aaagatcact
    61801 cgggcgcagt ctctatcggt aatctggaac gctacatcac cgataccgcg ctggcgatgg
    61861 gctggcgtcc cgatgtcagc aaagttgttc cccgtagcga aaaagtggcg gtgattggcg
    61921 ctgggcctgc agggttaggg tgtgctgata ttctggcgcg cgcaggagtt caggtcgatg
    61981 tctttgatcg ccatccagaa attggcggta tgctgacttt tggcattcct cctttcaaac
    62041 tcgataaaac ggtattaagc cagcggcgag agatattcac cgcaatggga atcgactttc
    62101 atcttaactg tgaaattggc cgcgatatta cctttagcga tttaacttct gaatatgatg
    62161 cagttttcat cggcgtgggg acttacggga tgatgcgagc agatctgccg catgaagatg
    62221 cgcccggtgt cattcaggct ctaccgttcc tgactgccca tacccgccag ctcatgggat
    62281 tgccggagtc tgaagagtat ccgctgacgg acgtggaagg taagcgagtc gtggtattgg
    62341 gcggtggcga tacgacaatg gattgtttgc ggacttccat ccgcctcaat gccgccagcg
    62401 tgacctgcgc gtatcgtcgt gatgaagtca gtatgccggg ctcgcgcaaa gaggtggtta
    62461 atgcgcgcga ggaaggtgtc gagtttcagt tcaatgttca accgcaatat atcgcttgtg
    62521 acgaagatgg gcgtttaact gcggtgggcc tgattcgtac cgccatgggt gagccggggc
    62581 cggatggtcg ccgtcgtcct cgtccggtag cgggttcaga gtttgaattg cccgccgatg
    62641 ttctgattat ggcctttggt ttccaggcgc atgccatgcc gtggttgcag ggcagcggaa
    62701 ttaaactcga taaatggggc ctgattcaaa ccggtgacgt cgggtattta cctacccaga
    62761 cgcatctgaa aaaagtcttt gctggtggtg atgcagttca tggcgcggat ctggttgtca
    62821 ctgcaatggc cgcaggaagg caggcggcgc gcgatatgtt aactctgttt gatacgaagg
    62881 catcgtgatg aaatcgttaa ttatcgttaa tccggctgac tgtattggct gccgtacttg
    62941 tgaagtagcc tgtgtggtcg ctcacccttc agaacaggag ttgaatgccg atgtcttttt
    63001 accccggctg aaggtacagc gactggacag catcagtgcg ccggtgatgt gccatcagtg
    63061 tgaaaacgcc ccttgtgttg gcgcttgccc cgtgggggcg ctgacgatgg gcgagcaggt
    63121 ggtgcagacc aattctgccc gttgtattgg ctgtcagagt tgcgtcagtg cgtgcccgtt
    63181 tggaatgatc accattcagt cgttgccggg ggatacccga caacaaattg tgaaatgcga
    63241 tctctgtgaa cagcgggaag aggggccagc ctgcgttgaa tcttgcccaa cacaggcgct
    63301 gcagttgctt actgaaagag aactcaggcg agtccgccag cagcgtattg ttgccagcgg
    63361 tgagaatcct ctctgaaaga aatctccgcg ctgaaaggcg cggagaatct attagattgc
    63421 ccaaccaccc gcgtaaaatg cgaccagtgc ggcagtaatg atcaccgtac caatattcag
    63481 cttttgccat tcgcgtgcaa agacgcggcc tacgaccagt gtcacaaagc ccagcataat
    63541 accggtaacg atattacaag tcagaacgat gaacacggca cacaccaggc cagccatagc
    63601 gtcaataaaa tcattgaaat ccagcttcga gacgttactt aacatcagca aacctacgta
    63661 catcagagcg ggtgcagtgg cgtaaccagg gatcaaaaat gacagcggtg ataaaaacag
    63721 aattaacagg aataacgccc ccactacggt tgcggttaaa cctgttttac ccccggcggc
    63781 ggttcctgcc gctgattcga tataaaccgc tgcgggcgct gcgcccacca ggccggagaa
    63841 tattgaactt actgagtcac tggtcagggc tttgccgccg ttgatgatct ggttgtcttt
    63901 atccaacaaa ttcgcctgac cggcgacggc acggatggtg ccagtagcgt cgaacactgc
    63961 ggtcatcacc aatgccagta cactcggaag tacagttggc tggagtgcac ccataatatc
    64021 gaggctgaaa atcagagact taccatcttc gccagtcagg cttggcatcg ccaccagacc
    64081 gtggtatttc accgctgggt caaagattaa gccgatgatc gaaattgcaa taatcaccaa
    64141 caagatcccg ccgggtacgc gacacttctc caggccgaag atgaccgcca gccccagcaa
    64201 gctcatcatc accgggaagg aggtaaacgc accgagcgcc actggcaagc cttcaatcgg
    64261 gtttttgata accataccca caccgttagc agcaatcagc agcagaaaca gcccgatacc
    64321 gatacctgta ccgtgagcga tacccatcgg caaattacgt aagatccagg tacgcacacc
    64381 ggttacggaa atggcggtga agatgacgcc catcagaaat accgcgccca gtgcgacagg
    64441 aacgctaatt tgttgcccga gtaccagact gaatgcggta aacgccgtca aggaaatcgc
    64501 gcaaccaatc gccattggca aattagccca taatcccatc agcaacgagc cgaagcccgc
    64561 gaccagacag gtggcaacaa acacagctgc gggaggaaaa cctgctttgc ccagcattcc
    64621 cggaacgacg ataacggaat aaaccatggc cagaaaggtc gttaagccag ccagtacttc
    64681 ctgacgaacg gtactgccac gagcggtaat tttaaaataa ttatccagcg cgccctgtgg
    64741 ctttggtgcg tccggtgttt gtaggatgtc tccagacata atgatgtcct cacgaggatg
    64801 ttaaagtttt attattagtt gcgttcgtac accagacgac catcaacgta ggtgcggtag
    64861 atcgaacggt catcgcccaa cgtcatcatc acgaacaatt tgtcgactaa agaaacagag
    64921 ttgtcatagc gcagctgctg tagcggagtg gcggtgggtt ccatcaccac gaaatcagcc
    64981 tctttgccag gtaaaaagtt gccaatcaaa tcgtcaaggc ccagagattt cgctccgccg
    65041 agcgtggcca ggtaaaacgc ttcatatgcc gagaggcgat agccttgtaa ttgcaatact
    65101 ttgtaggctt cgttcagcgt ttgcagcatg ttgaaagtgg ttccggcacc gatatccgtt
    65161 cccatgccca ctttaacttt cttctgccat gcttttttca agttgaataa gccgctgccg
    65221 aggtaaaggt tggaggtcgg acagaaagca atgctggatt tggtttcgct gagacgatcc
    65281 cactcttttt cttcgagatg gacgcagtga gcaaagacac agtttttacc ggtcaggccg
    65341 tactgatggt aaacatccag ataaccatca tggtcaggat aaagcgattt cacccaggca
    65401 atttcatctt tgttttcaca gagatgggta tgtacccacg tatccggata ttcttctttc
    65461 aggcgttgcg ccatcgccat ctgttcagga gatgaggtcg gggcgaagcg tggcgtaatc
    65521 gcatatagca gacgaccatt tttgtgccag cgttcgatca gttctttgct ttggtgatag
    65581 ctgctttcgg cagtgtcgag cagataatcc ggtgcgttgc ggtccatcat caccttaccg
    65641 gcaatcatac gcatattgat atgactggcg gcttcaaaca gcgcatcaac agattgcgga
    65701 tgaacagtgc caaacaccag cgccgtggtg gttccgttac gtaaaagctg cttgatgaag
    65761 aacgccgaca tttcgcgggc gtactctaaa tcctcataac gacgttcagt agggaaggtg
    65821 tgtttattca accactccag caattgctca ccataggccc ccaccatttc actttgcgga
    65881 taatggatat gtgtatcgac aaagcccggt actatcagtt tgccgcgata gtcgcgcacg
    65941 cgaatggtgt caggaatttg atgctttccg ttttcccatt cgccaaacca ttccactttt
    66001 ccctgtttaa tgagtaataa accatcctca ataaaccgca gcgcagaggc aatctcttcc
    66061 gggttatcga tcgtacgggt gacatcaata aaactgcctc gtaccgcttt taacgtgtgt
    66121 tctcctgaca tcattgactc ctttactgtt aatccatctc ttccgctgag gtaataccag
    66181 gcagaacgtt ttcctgtcgg tagccaccag ggaggataat atttaataaa atcgcagtta
    66241 acccgccagc acaaataggg ttttcaacta atacataaat agaggctggc aatattttaa
    66301 aaatttcggg atcgtaggag acgccaagcc ctaaacctaa agaagtggcg acaataagtg
    66361 tttcacggcg ctttaaaccg ttggtgatga tgatgcgaat ccctgcgatg gcaatcatgg
    66421 aaaacatcaa cgtcattgcg cctcccagaa ctgccgaggg aatggtcgtg aagaagccgc
    66481 caatcatcgg aaataagccg aggataacca gcattaccgc gatggttcgc ccgacataac
    66541 gtgaagcgac gccagtcatc tgaataaccc cattattttg cgcaaacgtg gttaatggta
    66601 atgaaccgac agcggaggcg ataacagaaa ccagaccatc tgccagcacg ccgcctttca
    66661 gccgggactg atactcttcc ccctgaatgg ggcggcggga aaccattgcc gtggcggtga
    66721 tatcgccgac tgcttccagc acgctaagca gataaatcgt gccaaccacc aggaactgat
    66781 ggaagctaaa actaaagccg tatttgaacg gatgcgggat ggtgattaac ggcaaattgc
    66841 gcatactgct gaaatccacc atgcccaggc ataacgatgc gatatagccg acacatagcc
    66901 caatggcgat ccctcccatg cgtagcaacg gactgcgaca gcagttaaag ccgatcacca
    66961 caattaaaac caataaacca acgccgagat gttcgtaatt gccgaacgta ccgctgcttt
    67021 tggctgcaaa tcctccacca aaatcgataa tgccgacttt aatcaggctt aagccgatca
    67081 tcagtaccac aataccgctg acggtaggcg taataacccg acgtaaatag ggcaagataa
    67141 atgaagatcc gacaaccaga aatgcgccaa cgaaggagac gccgagaagc gacgacatga
    67201 ttaactcttc gtgaaaaccg tcgcttttca tgctgctgcc cagcgcaatc atgaccgtaa
    67261 caaatgaaaa attgactgac tgaattgaga gtaggccaga accgacgatg ccgtagcggt
    67321 ttacttgtaa ccaggtacca ataccagagg cgatcatcgc catagaaaca agataggcag
    67381 ttgtttcagc ggaaagctgt aaggccgcac ccacgattaa cgcgggggtt accatcggaa
    67441 cgaaaattgc caacagatgg gtaatggcac caacgagagc ctgatgaaag ggagggcgat
    67501 cctccagttc aaatataagg tcagaacctg catggtttat atcagacatc ccttcactcc
    67561 ttttaaagta aatttgatca tctgaattag taaaataaat tcccacgaat ttggggcgca
    67621 ggacgccttg agaacggctt atctcatttt cgcagtcact atttagtcat cgttcaaaca
    67681 gagaaacagg ccgccgaagc ggccatttat tatttatatt ttttccagcg cagtgagtat
    67741 tttctccggt gtgaaatgcc attcgcgtaa ccagatgccg catgcatcgt gaattgcggt
    67801 agcaatcgcc ggagctgcgc catttacacc gatttccgag atcgatttcg ccccgaacgg
    67861 gccgacttta tcgtcgctcg gcaccagcac agcgcggaaa tcacgcggaa tgtcaccaat
    67921 tttcggtgcg ccgtaactgc gtaaatcacg cgttaacggg tgaccttcgg cgtcataaat
    67981 gatctcttcg ctcatactgt ggccgatagc acgcagggtg gcaccgtaga tttgtcccaa
    68041 cgctaactct ggattgaccg gtgtaccgca gtccagcaag gcgtagaatt tatccaggcg
    68101 gatttcaccc gtacgcgtgt tgacggcaac ttcagcgaag tttgcgccat acgggaaggc
    68161 gaaatcaggc gtgatataac tgccagttcc caccagtgaa ccaaagccgg tgccggtttc
    68221 gcctttatgg gcaatatccc cgaaactaac ttcgcctttc ttgccgcgca cgacgcccgg
    68281 cgttgccagt tgaacatctg ccactggctc acccaacatt tgcgcgccgt ggaacagaat
    68341 tttctcccgt agattttccg ctgccaaacg cgccgcgtta cccgagaagc aagtaccgga
    68401 cgaggcatat gcgcctttat caaacaacgc atgatcggta tcaccggaga taacatgcac
    68461 gtcctgcggt gggcagtgca gcacttctgc tgccagtttc gtcactacag tatccagacc
    68521 agtaccaata tccgcaccgc cagaatgaac gataaaggtg ccgtccgatt ccagtttgat
    68581 catgcagtta gcctgatcga tatccgggat acccgatttc tgcatgataa tggcgacgcc
    68641 gcgaccgatg tgccagtcac cattttgcgg tttcggtgac gaccattgga tcatctcgcg
    68701 accctgacgc aggatctctt ccagtgcaca gctggcggcg gaaggaacgg aggtcggcgc
    68761 tttaccttca ccgattgcac cgagaatttt cagctcttgc ccttcgtgta cccggttacg
    68821 ttcgataatt tccagttggt cgatctgtaa ctgttcagcc agttccgcta atgccatggt
    68881 gatagcgaag ttacctttcg gtgcgccata accctgataa gcaccatttg ggcaaatgtt
    68941 gctgtagtag gtggtgacct ggaaatcgac gttatcgcac ggatataacg gcagcgacag
    69001 cgccggtccg ttacacggta cggtgagtga gtggttgccg taagggccag tgttggcgcg
    69061 gaaatccatc ttcactgccg tcaggcgacc atcttttttc gctcccagtt tgacggtgac
    69121 tttcgcgacg tgacgagagg tgttagcaat aaactcttct tcacgggtgt agcggaacag
    69181 taccggacgc ccggtcacgc aggttgccca ggcgcacact tcttccagca ggatgtcctg
    69241 tttggaacca aaaccgccgc caactcgctc tttaatgaca tgaactttat gctgtttcat
    69301 gcccacgagg cgcgcgacct ggcggcgtaa gtgccatggt acctgggtgg aggcgtggat
    69361 aaccagacga tcgccgtcca tacgagtaaa gcagatatgt gtttcagtcg ggcactgctg
    69421 cgcttgcgtt gagttatagg ttcgctcaat gatcacatcg gcatcggcaa agcctttgtc
    69481 catatcgcca atatgaccat gaatactggc ggcgatattt ttgcgagggc gagaaccgat
    69541 cgggaagttg atgatcatat gctcgccgcg ctgggctgca tggctgttat cgtcttccag
    69601 agtatctggc gcaccagcaa catacaccac cggttcatcg tgcacgacag gcgcatcttc
    69661 cgccattgct tcgtcgatcg acattaccgg cttaagcact tcatattcaa cgtcgatgag
    69721 cttcagtgct tcgagcgcaa tttcttcact ttctgcgacg accgcagcaa cacgatcgcc
    69781 gacgtgacgc attttcttgc cgaacatacg gcggtcaagc ggtgacggtt ccggtgcgct
    69841 ctgaccaccc ggggtatagt agatatccgg gcaattcagg tgagtaataa cgtgaacgac
    69901 gcccggtaag gcttcagctt tgctgacatc cagatgagta atcagtgcgt gagcgtgtgg
    69961 gctacgtaac attttaatga cgcaggcgtc agccgttacg cggtcttcaa catagcaggg
    70021 tttcgcctgc accattttcg cggcatcagt tttaggataa tgcttaccaa tgacttctag
    70081 gtcgtcacgg aaagtcggag cgatatcaat ggtggcctgc ggattatttt tacgtgcaac
    70141 cgccagttca atgacctgat agtattgctg ccagccagca tcgcggctga acaaaccaga
    70201 aagcgcgtcg tcgatctctt cgcgggtagg tgcggcgatg cgatcgagaa gatcggtgat
    70261 aatcagagct gcagctggat cgttataacc agactgcacc acgccaacat caaccattgc
    70321 ctgttgaacc agacttaact cgttccattt gcccagagat tctgcggtac gaatatctgc
    70381 cttctctaac tgtgcggcaa taagcaagga cgcgttaacg atattaccgt taaagattat
    70441 tgcgtcagaa ccggcaaacc cgaaaccatc atcactgttg cgtacagagt gcattcccat
    70501 gttaaacaac agcttttgca cgttttcgcc tggattaacg gttagctcct gaggcgcgcc
    70561 atttaaagta aagtggatga tcatacggct tcctccccag cctgttggca gtcggcatac
    70621 aggtcggcta ccagaactcc cgtgatatag cgtttatagg ccacgctgcc ccgcaaatct
    70681 tcctgcggga aaatggcgtt ggcgacagcc tgttcaagtg cattgccttc cagattttgt
    70741 ttttcgacat catgcagacg cagtgcttta ctggcgacgc catccagcgc aattcgcata
    70801 ccgtcgtggt ctgtcattgc aacggctgcc gtcacgacgg ttaaacctgc ctgagaacgg
    70861 ctaattttgc gggtcgcaca ggtgcgatac ggatctttaa tgataatttc ggttagcagg
    70921 cgatcgcatg ggcaggccag gtagtcctcg attgacagcg tttcgccgtt gccaaaaacc
    70981 agttcagcat ccagtgccaa caggacggga agcagcaccg actcttcctg gcgggcggcg
    71041 atttcaccac caatggtcga ctgattacga acatggcgtg agtaaacaaa accgagggct
    71101 tcacgcagcg ctgcaggaat aaatcgcgca tcacgcagtg gctgcaagcg agacattgcg
    71161 ccaatccgca gtgcaccgtt atcccagtca acccagtcca gttccagatc ctgtaaggaa
    71221 atggcaatct ttttatcggt acgggttggt gtagcattga gtttgctgcc cccggcgaac
    71281 cagacggctt catcctggta gcggcgcttc agttccagcg cctgttcgac agagtcgggc
    71341 ctgaaaaatt gttcaatcat cttctttcct tatttaaccc tcttccctgc gggaagaggg
    71401 catctgtcat ttatgccagc gcatccatcc gacgccacat actggcagcg gcttttctgg
    71461 cttgtgcata aatggaatcg caatcgaagt taaactgacg gtcttcatag accatcacac
    71521 cattgaccat cacgctgtga acgctgcctg agcccatacc gaaagcgata tgcccggcga
    71581 tattgtctgc cagcagcggc gtcggcgagt tgtaatcgca aatggttaaa tcagctttgt
    71641 aaccggcttc cagaagccca aatttcgcgc caaagttgcg gctcatcagt tcgttaccgt
    71701 tagtcagggc tttggcaaaa ctgtcaggcc acagcggacc acccgcatcg cgatgtttaa
    71761 agaaggcaaa tttcatctct tcaaacatgt ccgaaccaat gccgtccgtt cccaacgcca
    71821 gattgcggat gtcgctaagg tgatggttgt agccgacatg gttgttcatg tttgaacggg
    71881 cgttatgcac caggaacgca tcgcgctgat tgagtagggt gatgtcatct ttcgacaagt
    71941 acagcccatg agcgaccagc gttttgctgt cgatgagatc gaattgcgcc agtcgtgcca
    72001 gcaggtcttt gccgtaccag tggtgactgt aggaaacgtc gtaaaggtct tccgcagcgt
    72061 gaatatgcaa accacggcct gtggctttca cggcttcacg cagcatctcc agaccggcat
    72121 ccggcacggt aaacggcgcg tgagcaccga tatgtgcttc caccagatac ggctcgcttg
    72181 tcgctttctt cgcctcatca atcaaacggg cgaaacggat gttttcttct acaccttcct
    72241 gcaactcttt gatgccgttg ttacggtcag tagtttcaaa acaggtcatc gcgcgcaggc
    72301 caacttttaa aaatgcgtcg cgcaatgtgg agagcgaccc gccgatatac gccggagagg
    72361 catggtgatc gataaccgat gtacatccgc tcttaatcgc ttccagggaa caaatcagtc
    72421 cgctgtaata gagcgactct tcatcaaggg cgcgatcgag ccgccaccag agatttttca
    72481 gcgttgagat gaaatccggg caaggggcga tgtttgccat aattccgcgg gaaagccccg
    72541 agtaaaaatg gttgtgcgag cagacaatcc ctggcatcac aatccggcca tgcatctctt
    72601 tgaagctggc gtcggggtag cgttgcgtca gggcatcgcc gatagcgaca atcacatcgt
    72661 tttcgatggc gatatcaacg ccttcctgca cttttgccgg gtgtagctgt accgcagtga
    72721 cattcttcag aatcaacatg attacacctc cacgcggccc agcagataat gatgatgctg
    72781 gtggacatgg ctgatgatgc ggcacatatc gttcagctcc ggtggtacgt tgttaaactg
    72841 acctttgctg tcgatgttta acacccagct ttggttattc agacgtactc gtacccggca
    72901 atcttccaca aggaagcctg ggttgctgct gttatcaaag tcttgcgcca ggctgaagac
    72961 ggtgattttg tctttgtacg gtttaccgtt ccacggacag aactgagcgc agttgccgca
    73021 ttcgttacag taagcgtcga ggtgcagcgt ctggaaacgg ttctggaagc ctgggaccgc
    73081 aatggagacg ttggcgcggt tcgggcagac atccacacac ttgctgcaaa cgtagttaca
    73141 ttcgaggcag cgagcggctt cctgggcgac aaacgcgtca cgatcgtcac tgttcaccag
    73201 cgtgatagag atatcgcctt tacgttgata gatttccgct ggattgacgt tgttccagta
    73261 tttatcgttc tggtgggaac ggatattttc ccgactaagg atggcatcgg tcgcccgacg
    73321 cgcggttccg acagcagcga caatggagga tggtccgcgc tgtacgtcgc cgatcataaa
    73381 gacgtcagtc agacgagttt cgccattatg gtcgacgtct ggccagccgt ttttgtccag
    73441 cggcacgccc atcgcattca gggcttcagt atcctgctgt tcaccaatgg cggtgatcag
    73501 gctgtctaca agcagtgtta ctgtttcatt ggtttcaacc ggacgacgac gacctttctc
    73561 atccggttcg ccaagcgaca taacgcgcaa ggttaaggtg ccatcagcat cgaaacgttc
    73621 cggattattc aggaaacgga actctacgcc gtcgtgcaac gcttcttcat actcttcgcg
    73681 ccatgcgggc atctcttgta gtgaacgacg gtaaacgatc gttgcttttt ctacgcctgg
    73741 aacgcgtaac gccgcacgag cgcagtccat tgcggtgtta cccgccccga caacgaccac
    73801 atgtttgccc agcttgagcg ctgtaccctt gttgtattca cgcaggaagg ggagtgattt
    73861 ccagacattt tggttgtcgc ccgccagttt cacaccgcta tttttatcag tgccggtggc
    73921 aatcagaaca tagtggaagc cctgattttt taactgctca atggttaaat cgggtgagca
    73981 gccatactca aatttcacgc cgtgagcggc aacaaaatcg atatcgtgct gaattaactc
    74041 tgcaggaata cggaactgag gaatgatatt tttcaccacg ccgcccgcat tggcttcgcg
    74101 ttcaaacagc gtaaccggat ggcccgctct ggcaaggaag taacctgctg ccagacccgc
    74161 cggacctgca ccaatcacgg caaccggatg gcgtgaacca gaaccggctg gtttgtgcca
    74221 gcgttgctta tattcatccc aacctttttc cagcgcgact tttttcagtt cgcggatatt
    74281 cagcgcactg tcgtaatcca ggcgggtaca gttgtattgg cactggtgat cgcaaatatg
    74341 accggtaatg gcgggcagag cgttgcgttg gtagatgagt tccagcgcgt cggcatagcg
    74401 gtgttcgcca agcagacgga tgtattccgg aatatcttgc ttgatagcgc aggcagtaac
    74461 acagggggca acgtagcagt cggtcagcgg caggtcttct gccacttcaa tacgctcttc
    74521 tggcttccag tgtttctggg tgtattccat ggttaacgca tctgctgcca gtctgttcag
    74581 tcgttcgacg tcaacatggt caagtcccca ggcgtcggag ccttccagct cgcgcatgca
    74641 ggcacttaag cgcagatagc cgccaggttt cagcaggtcg gttgccatag taatagggcg
    74701 aatacctgta tcaaaaatat cgcggatagt cagctgactg gcaccaccgg aataagaaat
    74761 gggcagtttg ccgtcaaagg cgcgagagag aactgctgca acattgatgg agagcgggaa
    74821 cagcgcacgg cctgacatat acatctcttc accaggcagt gcgcctttat tgttgatggt
    74881 gccgagagtg ttagtcagtt ttacgccaaa gccgagtgat ttttcttttg ccagtgccat
    74941 caggcgttcc agcatttcca gtgcttgcgt cagcttgagg tcgtgatcaa atgactcttc
    75001 ttttaagcct atgtaaccga aaccgcagac atcgaggatc tcacgaacac gcgcgtaccc
    75061 cagtaaggtc gggttaagtt tcacaaaggt gttgagccct ttttcttcca gcatgtagcg
    75121 gcaaatggct tcgatttcat gcggaggaca gccgtgcatg gtggagaggg tgacgccatg
    75181 caccatactg gtggggatgc gagcgggtaa ggcttgcaag ctttcgcgtt tttcctgcaa
    75241 tccgtggcga gctaaaaatg cgtcatcctg gagtaattta ttcagcgtat cgcgatattg
    75301 agcgaatttc ggatggtcag atgcgtccat catattgtcg atgaactgtt gcatcggcgg
    75361 ttgcttaata ccttcgaggt tgtaaccgac gctcatatta aagatgaacg atttaccgga
    75421 atcagaaggc tggaacatcg cttcgagaag gtgcagggca aaccaggctt tgaggtattc
    75481 atcccaggct ttaagcaggg taaattcggt agaccattcg gtgttaaagc actcgtcttc
    75541 ggcatcgata cagggctttt ccagctccag gcggtcaaga atttggacgg tttttagttc
    75601 gatgaagcgt ccgccagtca gccaggaagt gacaatattt tgcgcgagct gcgtgtgcgg
    75661 gccagcggca gggccgacgg gagtggcaca ggtttcaccg aagacgctaa cagttttacc
    75721 ttttacaggt gagtaaaact gttgctcggg aataccaaag attgagcgtt gttgttggta
    75781 ttcatcaaat atgcgcgtca aaagttcctc aaacggaatg ggacgcataa tatcccccat
    75841 aaccctctcc ttagcttata cagatatcat tgagtgaccg gtgttgagaa atccggtggt
    75901 ttagggggat agagcaacaa tcgcaccact cttgccaggt ttggcgggaa atataaaaaa
    75961 atgtgaggcg gctcgaaact taagggcttt ttgtgaatga actcacaggt gctaaatgag
    76021 aattaaaatc actcctgaaa attattattg ccagcccatt tatcaatttt tctcttttct
    76081 caaaatcagt gattccttat cattatgata atgagtgccg gaaggattaa atatttgaac
    76141 gcaatcgttt aactacgttt tctaaatttc agtatacctt tccttcgctg taataaagtc
    76201 gtccggagta tcaatatcta aaattatttc tgcattttct atttcgacgg aataatgttc
    76261 tcccataagg agtgcctgac gcatattggt gacattgggt cgttggattg cctgcatcag
    76321 gcatggtttt gacactaaaa tcggatggcc ggggatgcca ttatggagtg gcagtattgc
    76381 gccatcattt cgtaacgacc agatttttct aaaaatatcg atggtgaggg ttggcatgtc
    76441 accgtgggtg agaaaacaat gttctgtttg cactgcgggt actgcggcct tgacggatgt
    76501 tagtaaaccc tgcgcataat ctgggttgtg aataatagtg atattgctct ggttcgcata
    76561 acgttcgtgc agttcattac cacgatagcc ggtgactaaa ataattcggc tacaaaactg
    76621 caacgcattt ttgatacttg tatcaagaat tgttccctgt tcccagggta acatcatttt
    76681 ccattgcccc attcttgatg ataatccggc agcggtaatt atacagtcga tggctgacat
    76741 agtggttcca ttatttatag gggctaataa cgcctgataa ttgcgttact tcctgatgga
    76801 ttaagttggg gataatttat cccagagagg tcataaagac tcgtgaaaag tatagttgac
    76861 ccatcggcat tagtcattga tttaggtgcg cagaaacgtc ccactgtgat ttctgttgtt
    76921 ggtgctgggg ggaaaaccag cttgcttttt tggctggcag aactgctcca ggctagcggc
    76981 aggcgtgtgt taatcacgac gacgacacat atgtttatgc caacgtctca ctggcccgtg
    77041 gttttctgtc gtgatcccgc catgcttcct catgcgtctc ttacatcccc catttcattt
    77101 tgttttcaca gctggaaagc gaaccaggga aaagtgcagg gatttacgcc agaagcgatt
    77161 gatgcactgg tgcaacgacc agagtgtgac gtaattctca ttgaagcaga tggctcgcgt
    77221 ggaatgccgt taaaagcgcc tgatgagcac gaaccttgca tacccaaaag cagttgctgc
    77281 gtgattgctg tgatgggagg acatactttg ggcgcgaaag tgagcacaga aaatgtccat
    77341 cgctggtcgc agtttgctga tattaccggg ttaacacctg atgcaacctt gcaactgagc
    77401 gatctcgttg cgctggttcg ccaccctcag ggggcgttta aaaacgtacc gcaaggttgt
    77461 cggcgagtct ggttcattaa ccgtttttct caatgtgaga atgcgattgc acaaagcgag
    77521 ctccttcaac cgctgcaaca acacgacgta gaggcaatct ggctgggcga tatacaagaa
    77581 catcctgcaa tcgcgcgcag atttgtgaat tagcggcccg ggtattccgg gttactgaaa
    77641 ggatatccat tgaggttggt atgaatattt tcacagaggc tgcaaaactc gaagagcaaa
    77701 attgtccgtt tgctatggcg caaattgttg atagccgagg ctcgactccc cgccattctg
    77761 cacaaatgtt agtgcgcgcc gatggttcta tcgtcggtac aattggtggc ggaatggttg
    77821 agcggaaggt gattgaagag tcgcttcagg cattgcagga acgtaagccg cgattattcc
    77881 atggacgtat ggctcgtaac ggtgcggatg ctgtcgggtc agattgtgga ggtgcaatgt
    77941 cagtatttat cagcgtccat ggtatgcgtc cacgtctggt gttgattggc gcggggcatg
    78001 tcaaccgggc gatagcccag agtgcggcgc tattaggatt tgatattgcc gttgctgata
    78061 tttatcgcga aagcctcaat cctgaactat tcccaccatc aaccacgctt ctccatgctg
    78121 agtcgtttgg tgcggcagtg gaagcactgg atattcgccc tgataatttt gtcctgattg
    78181 ccacgaataa tcaggatcgt gaagccctcg acaaactcat tgaacagccc attgcatggt
    78241 tggggttgct ggcaagtcgt cgcaaggttc agcttttcct gcgtcaattg cgtgagaaag
    78301 gcgtggctga agaacatatt gcccgtttac atgcgcccgt tggttacaac ataggtgcgg
    78361 aaacgccgca ggagatcgcc atcagcgtgc tggcagaaat attacaggtg aaaaataacg
    78421 cgccgggtgg gctgatgatg aaaccttctc atccttccgg acaccagctg gtggtgattc
    78481 gcggtgcggg agatatcgcc agtggtgtgg cgctacgtct gtatcatgcg ggttttaaag
    78541 tgatcatgct ggaagtggaa aaaccgacag tgattcgttg taccgtggcg tttgcccagg
    78601 ccgtgttcga tggcgaaatg acggtcgaag gcgtcactgc tcgcctggca accagctctg
    78661 cggaagcgat gaaacttacc gaacgcggat tcattcctgt gatggtagat cccgcctgtt
    78721 cattgcttga tgaactgaaa ccgctttgcg tggtggacgc tattctggcg aaacagaatt
    78781 tgggaacgcg ggcagatatg gcaccagtaa caatcgcgct tgggccgggc tttactgctg
    78841 ggaaggattg tcatgcggta attgaaacaa atcgcgggca ctggctcggt caggtgattt
    78901 actctggttg tgcgcaggag aataccggtg ttcctggcaa tattatgggg cataccaccc
    78961 gacgggtgat ccgtgctcct gctgcaggca ttatgcgatc caacgtgaaa ttaggcgatc
    79021 tggtgaaaga gggcgatgtg attgcctgga ttggtgagca tgaaattaaa gcaccgttga
    79081 cggggatggt gcgtggcttg ttgaacgacg gcctggcagt ggttggtggt tttaaaattg
    79141 gtgatatcga tcctcgtggt gaaaccgctg atttcaccag cgtttctgat aaagcccggg
    79201 cgattggcgg cggcgtactt gaggcgttaa tgatgttgat gcatcagggc gtgaaagcga
    79261 caaaagaagt gctggaagtg gcttaaatga aaatggccgg atggtattac ccacccggcc
    79321 tgagactgat gacaaacgcc aaattgcctg atgcgctacg cttatcaggc ctacaagtgc
    79381 attgcaatct attgaatttg cgatgttttg taggacggat aaggcgctcg cgccgcatcc
    79441 ggcatcaaca aagcgcactt acttgacaat ctttggccgg atggtattac ccacccggcc
    79501 tttaaattac tgacaaatca ccgttcctgt tttgccttca atcccttctt tcgctttgct
    79561 caatacggta atcagtgctt cacgacctgc gcgagagcga gcaaacgagg cggcagcttc
    79621 cactttaggc agcatagaac ctttcgcaaa atgaccttct tcgataaagc gttcagcatc
    79681 acttaatgac aggcgatcga gccactgttc attctcttta ccgaagttaa tggccacttt
    79741 ttctaccgca gtcagaatga tcagcatatc ggcatcaatc atttctgcta aacgggcgct
    79801 ggcccagtct ttatcgataa ccgcgctggc accacgcaga tggttacctt cacgaataac
    79861 agggatacca ccaccgccaa cggtgatcac cacctgacct gcatctacca gagctttaac
    79921 cgtttctttt tcaatgatat caaccggttt tggcgaggca acaacacggc gatagccgcg
    79981 accagcatct tctttcagag tgtaaccctg ttttgtcagt tgctctgctt cttgctcggt
    80041 aaagaacgaa ccgatcggct tggtcgggtt gaggaatgcc ggatcgttag cgtcgacttc
    80101 gacctgagta accagcgttg ctacaggttt attaatgccc cgagaaagca gttcttctct
    80161 taaagcgttt tgtagatcgt aaccaatata accctggctt aatgcaacac agacagacat
    80221 cggcagcatt ggcgagtgcg cttcagtttt tgcggcagct tcaaaggcct ggttaatcat
    80281 gcccacctgc ggcccgttac catgagtgac gataacttca tgtccctggg caattaaatc
    80341 aacaattgcc tgagaagtaa tttttaccgc tttcatttgc ccggccaggt catcgcccag
    80401 cgcattcccg cccagggcga gaacaatttt cttactcatt tttcctcact catcattctt
    80461 gattgtgtat agatccctgg cagtggttag agcacgggag ggacaaacgg tttgcgtcgc
    80521 aggaaacgac ctcgcccggc tttgcctgta aaggtgccgt cacagaaaat cgtttcacca
    80581 cgggataagg ttctgacaat cgcgccctga caggtaaaac cctcccatgg cgagtagtcg
    80641 gcgttgtcgt ggagatggcg atgctgaatt tgttggctct gacgtgggtc gataatcacc
    80701 acgtcgccgt cggaaccggg cgctaataat cctttttgcg gccacaggcc aaacaacctg
    80761 gcgggcattg cgctggttaa ttcaacaaag cgttccggtg ttatacgtcc cgtcatcacg
    80821 ccactggaaa acagtaactg catgcggttt tccacaccgg gtaagccatt tgggcagcga
    80881 ctgaaatcgc ctttagaaat ttgcaggcgt tgagccatcg agaaggtgca gtgatcggtt
    80941 gccaccacgt caatcgcacc atcgctgatg ccacaccaca gtttgtcctg ctcgcgtacg
    81001 ttacgcagcg gtgggctaag aatgaacttc atgccatctt ctgtatcgta actgcgttcg
    81061 tccaacagga gatattgtgg gcaggtttca acccagactg gctggtgatt cgcacgggca
    81121 agacgcagat aatccagacc taagccgtta gacaggtgca cgatatagag cggggcgtta
    81181 ccggcaattt gtgccaggtt aatcatgcgg gcgatggctt ccgcttcgca ttccagaggg
    81241 cgactcaagg catgatagcg cggcgcggtt aaccctgcgg cgataaattc cgcccgcttg
    81301 ctggcgatag ccgcatcatt ttccgggtgc acggtggtca gcgcgccgga ttcatgcaag
    81361 cggcgtaatg cctgcaaaac ctcgtcatcg ttgagtttgt attgataggt taaatagagt
    81421 ttaaaactgc tcagtccttc ctcgaccatc atcgggattt cgtcgaggat tgcgtgatta
    81481 atgtgctgga tcacaccgtg aaagctgtaa tcgatgaccg ctttatgggc ggcataacca
    81541 cgataaacct ccagttgatg gcgtaaccga cagccgtttg ggccaaatcc catatggtca
    81601 ataatggttg ttgtaccgcc acacgcagct gcgcgggtac cggtaaaaaa atcatcacaa
    81661 ctgcgcgcga tgccgacatc aatattgaaa tgcgtatgga catccacgcc gccagggaaa
    81721 acgtaacagc cagtggcatc aatttcttca tacgggagct gcggcgaaat attgttgccc
    81781 aactggcgaa caatcccgct ttcaatcagc aaatcctgtt tggcttgtcc atctgcgtta
    81841 acgacagtgc cgtttttgat caatacgcgc atagcaaact ccaaaaaccc tccggcgaac
    81901 cggaggggaa taagtgaaga gtaattactc tgttgccagc cagcttaacg ggattgcagc
    81961 gtacatcgcg gcacaggtca ccaggtgaga tttccaggtt ttttcgttag gtgcatgcgc
    82021 ttcaggttct ttacccgggc caaagccgat caccggaatg ccgtgacggc ccatgataga
    82081 tacgccgtta gttgagaagg tccacttatc aacaaccggc gctttgccaa acagcccttc
    82141 gtaggcattc accagtgctt taacggtgaa gtgatcttct tccactttcc aggtcgggaa
    82201 gtagcattcg gttgggtaaa ccaggccagt ccaggacgga cggtcgtagt tgtacataga
    82261 aacaaccgcg ttagctttct gtactgcagg cagggcgcgg atttcgtcca gcgcgccttc
    82321 ccaggtttcg ccccaggtca gacgacggtc gattgaaact gcgcagctgt ctgctacagc
    82381 gcaacggctt ggggatgtga agaagatttc agaaacggtg agggtgcctt tgccgaggaa
    82441 ttcgtcataa cccagacgtt gggagagttc ttgtaattcg ccaagaatcg gacccatttt
    82501 gaaaatggcg ttatcaccgc gttctggtgc agaaccgtgg cagctaacac cctgaacatc
    82561 aatacgaatt tccatacgac cgcgctgacc acggtatacc tggcagtcgg ttggttcggt
    82621 actgaccaca aattccgggc gaatgccgga ttgttcaata atgtactgcc agcacagacc
    82681 gtcgcagtct tcttcctgca ccgtaccggt aaccagcagg gtatattcat cttccagacc
    82741 gaggtcttta atgattttac cggcataaac catagaggcc atgccacctt cctggtcgga
    82801 agtaccgcga ccaccaatca gttcatcagt ttccatgcct tcgtacggat cgaagtccca
    82861 gtttttgatg ttgccaatgc cgacggtatc gatatgagcg tccattgcca ccagacgcgg
    82921 gccgtggccg atataaccga gaacgttgcc catcgggtcg atttcaactt tatcgaagcc
    82981 gactttttcc atctcttctt taatacgatg tactacgcgt ttctcgtcgc agctttcact
    83041 gggaatagca accatgtcgc gcaggaagcg agtcatatcc gcctggtaat cttttgcttt
    83101 ttcaagaatc agtttgaatg gaatattctt agccattatt tttctccaga ttttttactc
    83161 cccgttttgc agggagcagt tccaataggg tgattaaggt gctacagcgt gtttgccttc
    83221 ccagacaact tcgcggtagt gcttcacgtc ggtgtcgcct tcagtgctga taaccagcac
    83281 cacggcatct ttgttcagcg ccagtttttc catcaggctt tgacgttgcg ggtgataatg
    83341 aaccgctgcg agaacgccca aaccgacagc gccggattca ccggagatga tgcgcgggtc
    83401 gttgccgtac ggattaccca gcacgcgcat acctaatgcg gcaacgctgt cctggcagga
    83461 gatgaattgg gtggcgcagt tacgtaggat ttcccagccc agcgggttag gttcgccaca
    83521 ggccaggcct gccatgatgg tggccatatc accgccaacg ttgacgatgt cgcctttgac
    83581 gccggagcga taaatacagt cagctttgtc aggttcaaca ataatgctgt gcagattttg
    83641 cgggctatag acgtcgacca gataacccag cacaccaccg gccattgctc cgacaccggc
    83701 ttgcagcaga acgtgcgtcg gggttacgcc catttcacgc atttgctcga cggcttcatc
    83761 tgccagggtt gcgtagcctt gcatgatcca ggttgggatt ttggtgtaac cttcccatgc
    83821 cgtgtcctgt accacttccc agccgtgctg ctgcgcgtgt tgcatggtca ggcgaacggt
    83881 atcgtcatag ttcatatccg tgacgatgca ctcggcaccg aggttcagaa tggcgtcaac
    83941 gcgttcctga gcagaacctt tcggcatgta aatcaccgca ttctgtccga gttgctgtgc
    84001 tgcccacgcc acaccgcgcc cgtggttgcc gtcggtggtg gtcgcgaaag tcattttttc
    84061 gccgatggca tttttcaggt gctcaaatga cagcgtttcg atatcaagat gatatttttc
    84121 acacaataat tgagcgatgg cgtacgcacc gccaagcatt ttgaaggcgt tcagaccgaa
    84181 tcgttttgat tcgtctttga cgagaatttt cttcacacca aaaaggtttg cgagatcgtc
    84241 cagcgcacaa agcggtgttg ggcgataacc agctattttc tggtggaact ggcgcgccag
    84301 tttggcctgg ctttgcgaaa agagcggtga tgtttcgccg ttgaaaaatt tgttatcggc
    84361 gatatcaatc ttcaatgaga aaacggacat atcctatcct tttggcctct ggaaatagag
    84421 ggccgaccag ctgatcggcc cgaggggtta tttcacgcgt tcttgcgctt ctttcagcag
    84481 ttgctcgagc agtgcacctg gtttggcgta tttacgggac aggatcatcg cggcgatgat
    84541 ataaggcttc cagctggctt ctttgtaggt agcgatacgg tatttttcga atacgccttc
    84601 agtcacttca ccttctttac aggatacgcc gctgatatca gctggcaggc agtgcatgta
    84661 cagggcttcg ccatcacggg tcagttccat catctcttca gtacaatgcc agtctttgtg
    84721 ttgcgcgttc tgtgccagac actgtttttc cagtgctttt aagccttcgt gatcgttcgc
    84781 acgcagcaat tcagtacgct cttccatcac tttgtaaggt gcccatgact tcggataaac
    84841 gatgtctgcg tctttgaagg cttcttccat gctggtgacc tgacggaagc taccaccgga
    84901 ggctttagca ttgtttttcg ccacttcaac cacatccggg atcaggtcgt agccttccgg
    84961 atgggccagg gtgacatcca taccgaagcg agtcatcaga ccgatgatgc cttgtggtac
    85021 agagagcggt ttgccatagc ttggagagta ggcccaggtc atggcgattt ttttaccttt
    85081 caggttttcc agtgaaccaa agtgttcacg taaccacgcg aggtcagcca ttgactgagt
    85141 cgggtggtca atatcgcatt gcaggttcac taaagccgga cgctgtggca gtacaccctg
    85201 cttgtaaccg tcatcaagtg cagcgccaac ttcacgcata taggcgttgc ctgcgcccag
    85261 atacatatcg tcgcgaatac caatagcgtc ggcgcagaag gagatcatat tggcggtttc
    85321 acgcacggtt tcgccgtgag cgatttgtga tttgccttcg tcgagatctt gttgtgcaag
    85381 gccgagcagg ttaagcgcgg aagcataaga gaagcgggta cgggtggagt tgtcgcggaa
    85441 tacggaaata cctaatccac tattaaagac tttggttgag atgttttcag cacgcagtgc
    85501 ttttaatgct gcggcaacgt ccagtacttg tttcagttca tctggcgtct gttcccacgt
    85561 taacaaaaaa tctttctcgt gaaggtgaga ggtcagcgaa ttgatatcct taatcagctc
    85621 attaacagtt ttcatcataa agtcccggta gtggaatgac aggcaaagtg gtttatttca
    85681 ctgtgacttg ccaaagtgat tggtgttttc acagctatac ctgggactaa caataagtgt
    85741 gccagaatta tccgcgcggc tgcaaaagac taatttgacg gataatgtgt ggggttgatc
    85801 acaaattgat gccagcttct ggttgtttgt aaaatacgaa aacgtttggc tttttttatc
    85861 gcgttattta tttgctgcta agtatcagaa ataagataaa ggggcaggct atctctcttc
    85921 agtttgataa actcaccgcc gtaattctca tattgatata gaaatgtgat atctcttcca
    85981 taatttcatt agaaaatgat aaaaatctta tgtttttatc aaagccaatt tcgcctgagt
    86041 taatatcatc atgatctgtg aaccatcaac gtcttcaatg atcgactttt aagtaagtct
    86101 ggaaggtaaa gctatggagc ttgctactac gcagtcagta ttgatgcaaa ttcaaccgac
    86161 aattcagcgt tttgccagaa tgcttgccag cgttttgcag cttgaggttg agatcgttga
    86221 tgaaaacttg tgtcgcgttg ccggaacggg cgcgtatggg aagtttcttg gtcgccagtt
    86281 gagcggcaac tcacgcctgc tccgccacgt cctggaaacg aaaactgaaa aagttgtgac
    86341 acagtctcgc ttcgatcccc tttgcgaagg ttgcgatagt aaagaaaatt gccgcgaaaa
    86401 agcatttctg ggtacgcctg tcattttaca ggatcgttgt gttggggtga taagtttgat
    86461 tgccgttacc cacgagcaac aagagcatat cagtgataat ttacgcgaat tttctgatta
    86521 tgttcgccat atatccacca tttttgtttc gaaacttctg gaggaccagg ggccaggaga
    86581 taacatcagt aaaatatttg cgaccatgat cgataatatg gatcagggcg tattagttgt
    86641 agatgatgaa aatcgggttc agtttgttaa tcagactgcc ttaaaaacac ttggtgttgt
    86701 gcaaaataat attattggga aacctatccg tttcagacca ttaacatttg agagtaattt
    86761 tacccatgga catatgcagc atattgtttc atgggacgat aaaagtgaat taatcattgg
    86821 tcaattgcat aacattcagg gccgacaatt atttttaatg gcgtttcacc aatcgcatac
    86881 cagtttttct gtagcaaatg cacctgatga accgcatatt gaacaattgg ttggcgagtg
    86941 ccgtgttatg cggcaattaa aacgactcat tagccgtatt gcacccagcc catccagcgt
    87001 tatggtggtt ggtgaaagcg gcacgggtaa agaagtcgtc gcccgtgcaa tccataagtt
    87061 gagcggaaga cggaataaac cctttattgc tatcaactgt gccgcgattc cggagcagct
    87121 tctggagagc gaactgttcg gttatgttaa aggtgcattt actggcgctt ctgccaacgg
    87181 taaaacaggg ttgattcagg cggcgaatac gggcacgctg tttctcgatg aaataggtga
    87241 tatgccatta atgttgcagg ctaaattact gcgcgctatt gaggcacgtg aaattctgcc
    87301 gattggtgcc agtagcccaa tacaagtcga cattcgcatc atttctgcaa ctaatcagaa
    87361 tttggcccag ttcattgccg aaggtaaatt ccgcgaagat ctcttctacc gacttaatgt
    87421 tatcccgata actctgccac cgctgcgtga acgtcaggaa gatattgaac tattggtgca
    87481 ttacttttta catctgcata cccgtcgtct gggatcggtt tatcctggca ttgctcccga
    87541 tgtcgtcgaa atattgcgta agcatcgttg gcccggaaac ctgcgcgagt taagcaattt
    87601 gatggaatat ctggttaacg tggttccttc aggtgaagtt atcgacagca cgctattgcc
    87661 gccaaatctg ctgaataatg gcacaacgga gcaaagtgat gtaacagagg tcagtgaggc
    87721 gcacctgtca ctcgatgatg cgggcggcac ggcgctggag gagatggaaa agcaaatgat
    87781 ccgcgaggcg ctttcacgtc ataacagcaa gaagcaagtt gctgatgaac tgggcatcgg
    87841 cattgctacg ctctatcgca agattaagaa atatgagttg ttaaacacat aacctcagtg
    87901 aatccggcct gaattcaggc cggatatcct tttacttcgt tttctcgcaa tccagaactg
    87961 tatttacaat catctgatac cccgtgcagc gacaaagatt tcccgccagt ccgcgacgaa
    88021 tttccgtaat ggttaatggc ttctcgcgtg gtttcgccag cattgccgtg gtagccataa
    88081 tcaggccagg cgtacaaaac ccgcactgca ctgcgccgga tttcgcataa gcctgctgaa
    88141 catgagaaag ttttccgcct ttcgcttcac cttccagcgt gcggatctct tttccttcag
    88201 cccaggcggc aaggtataag caactgtcta ttgctgtgcc gtcgaccaac accgtacagg
    88261 caccacattc acccacgcag cacccttgtt tgacacttag cagtccttgt tcgcggagta
    88321 attccgagag cggcgtgcct ggtgcggcgt gaagctgaaa aggcatcccg ttaatggtgc
    88381 attcgatggt aattgtttcg ctgtgattca ttgcaatttt ccccccgccg cggcgacggc
    88441 ttcgctaatc acttttttgg tcatcgtctg gatgagatgc agacgaaact ctttactggc
    88501 ccgccatgaa gaacgcgggg cgacatcttg caggacagat tcgctgatag cttccagcgt
    88561 ttgcaggttt aatggcgcat tttgtgcagt ctgttcggca tgttggcagc gaatcggcgt
    88621 tggcgcggca acaccaaatg ccaggcgtaa ttcgctgaaa ttgccgttat ccagtcggca
    88681 atgtgcggca cagccaatcg ttgaaatatc cattgcgtcg cgcatggcat atttaaaatg
    88741 cgcgctgccc gcgtgttctt tcggctgggg cggaaaatga aaggcgacaa ggatttcgtc
    88801 atgctcaaga gacactttgc ccggcccagt gtgaaagcca ttaatcggga cgaaacgaac
    88861 accgcgtggg gagtggatct ccagctttgc gtcataaatt agcgttggcg tggcagaatc
    88921 tgcgctggtg gcaccgttgc aaatatttcc accgtaggta gcgacgttac ggatctgcgg
    88981 tccggcaatg gatgaggccg cagcacataa cgccgggaga tgacgttgag ttacaggatc
    89041 ttcaatgagc tgggtaaatg tcgttgcaga gccgatacgt agtgagccat cttccgccag
    89101 cgtaattccc cgcagctccg ccagattatg aatatcaaca atatggcgat aacggtcatt
    89161 gtgatggtgg agctgaatca gtacgtcagt gccaccggcg agcagtttgg cctgcgggtt
    89221 gtcagccagc aggttgatgg catcggcaag ggttgctgcg cgatggtaag aagcaaaatc
    89281 aaacatgatg ttatcctcaa atcaatcctg ccagatggaa ctcttcatat aaccgttttg
    89341 gcgtcagcgg cagtgtattg attgcaacac cggtagccat cttcaccgcg ttacgaatag
    89401 cagcggcaac aggaattatt ggtggctcac ccagtgactt atgtccgtat gcggattgcg
    89461 gctcattgat ttcgacgaac gcgctttcca gttgtggcag atccggcatg gtcggcattt
    89521 tgtaatccag cagattgggg ttacggacca cgccgctttt cgcatcgatg atcatctctt
    89581 caaatagcgc ccagccaatg cccattccca ttccgccgtg tacctgacct tctgccagca
    89641 gcggatttag aatatgcccc gaatcatgaa cgttgaggat gcggttgatg gtgactttac
    89701 acagcgcaat atcgaccgtc agatcaacaa aggtacagcc aaacgccggt gggttagtgg
    89761 tggttttgat ggagctttca gcagagagct gcccaccgcg ttcagggtgg tagaaagcgt
    89821 ccatcgccaa atcttttaac gacattaacg gcccttccgg tcgttcaacc agcacgatat
    89881 ggccttttat cagggtcaga ttcatcgctg actgatgtag catgactgcg gcgtgagcga
    89941 tgattttctc ttttaataac agtgccgcac tgcgcagcgc aggcgcggca acatagctct
    90001 ggcgtgaggc aaatgcgccg ggatcgaacg gcgtaacatc ggtatcttgt gttgaaataa
    90061 cgcgaacgtc gctgaccgga acccccacgg tttctgccac catttgcgag aagacggtgt
    90121 cggcaccctg accgatttcc gtcgcgccgc tttgcacgtt gatggttcca tcctgattca
    90181 tcaacaggcg cgcgcctgct atttctacgc cgacaggcca ggtgttcgag gtgtagctaa
    90241 aacaggcgac gccaacgccg cggcgtaaat tgcctttctg gttctggcac tctgcacggc
    90301 gtttttccca ttcaaagatt ttccggcctt tttcaagaca ctccggcaac cctgcgctgt
    90361 aaatacgttt gcccgtgagc ggattagcat ctccttcgcg ggcggcgttg cgtaaacgaa
    90421 tttcaacagg atcaatacct aacgctgtcg cggcgtcatc aagcatagac tcaacggcaa
    90481 atacgacttg tggcgcgcca taaccacgca tcgcaccagc cgaggggagg ttggtatagc
    90541 aggtctttga actgtaagcg taggcacaac gaggataaag gtaagcgact ttattccccc
    90601 ctgcagaagc gatggagtgc ccgtgagatg cataagcgcc ggtgttagac agaacatcca
    90661 gactataacc tttcaatgtt ccgtcgcggt tcacgcccat ttgcccgtca atagtaaaag
    90721 cgtggcgggt acgggttgcg aggaaacact cttcacggct aagggaaact ttcaccggaa
    90781 tgccgccaag cttgctggtc aggaatgccg ccattggctc ttccagtaca tcctgtttat
    90841 taccaaaacc gccaccgaca aacggtttga tgactcgtac gcatgaccag ggaatatcca
    90901 gcgcctgacc aaccacgcgg cgaacaatgt gcgggatctg ggtgctggaa acgatggtaa
    90961 ttcgcgagtc atcctccatc catgccagcg atgtcacgct ttccatgtga caatgttgaa
    91021 taacgggagt ctgatagtgc ccctgaacct ggtagtcggc ggcatcgatt gtttgttgga
    91081 cattgcccgt cgacatcgtg ctttgtttta gtaaattgcc gccgttatgg attggttcag
    91141 cgtcatcagc cagcgccgct tctggggtgg tgataacggg taattcttgc cactcaatgc
    91201 tgaccaattg cgccgctttt tctgccgtga gttcatcacg ggccacgacg atggcaacgg
    91261 cgtcaccatg gtgacgaaca tggcgagtta gcagtgcgcg atcggcggta tcgcgcttgt
    91321 tttcgtcaag tgtccaggca tgcccagctg tagcgaatgg gatatcaggc acatcttccc
    91381 aggtaaaaat cgccagtacg cccggtaaac ttctggcttg ttcatcatta atacttacgg
    91441 cataaccatg tgcgataggg ctacgtacat atttcgcgta acacatgccc gccataacat
    91501 aatcgtcagt atatcgtgcc cgcccggtga ccttagcaat ggcatctacg cgcatgcatg
    91561 attcacctgt agcggttgct tcccgcgctt ccatagaaat cccctcgatt tgaacatttg
    91621 gttcttatgg caaagattta gtctaagcag gcaagattta cgccagaaac gacgataagc
    91681 gttaagggag gaaggaagtg tgatgatgtt aaaaaaacac agatatgata cgaaacagga
    91741 ccctgaatag gggcagtgat acgtatcaat atgagtggcg ataaaatcaa tgtgataaat
    91801 tgggattatt acgcatcagg atgggaaata gggtaaaaag tcaggaagtt cgatacctct
    91861 acagcgaata ttttgctgat gatacttccc tttgccaaat gaaattaacc tctttctcta
    91921 ttaccacgtt tttccagaag caagagattg ggtatcaaga ggctggctgc tatgataagg
    91981 cgtcttgttt tttaagcgag gaaagatttt gagtgcggga cgcctgaata aaaaatctct
    92041 gggtatcgtg atgttgttat cggttggact gcttttggcg ggctgttcgg gtagcaaatc
    92101 atccgataca ggaacgtatt ccggctccgt ttacaccgtg aaacgggggg atacgctata
    92161 tcgtatttcg cgcaccacgg gaaccagcgt aaaagaactg gcgcgactga acggcatttc
    92221 ccccccttac accattgaag ttggtcagaa actaaaactg ggtggggcga aaagtagcag
    92281 tattacacgt aaatcaaccg ccaaatcaac gaccaaaacc gcatcggtta caccgtcatc
    92341 agcggtaccg aaatcatcct ggccgccagt agggcaacgt tgttggttat ggccaacgac
    92401 agggaaagtt atcatgccgt attcgacagc agatggcggc aataaaggga ttgatatctc
    92461 agctccacgg ggtacaccta tttacgccgc gggtgcagga aaggtggtgt atgtgggcaa
    92521 ccagctgcgt ggctacggta atctcatcat gattaaacac agtgaagatt acattacggc
    92581 ttacgcccat aatgacacga tgctggtaaa taatgggcaa agcgtgaagg ctgggcaaaa
    92641 aatcgccact atggggagca cggatgcggc atctgttcgc ctgcatttcc agattcgtta
    92701 ccgtgcaacg gcaattgatc cgctacgtta cttgccgcct cagggcagca agccaaaatg
    92761 ctgatggcga attaatcagc agtcagcagc gtggcacttg ctaaggagag cgtaaggttt
    92821 ataatgcctt acgcatctcg aagcgggcgt agttcaatgg tagaacgaga gcttcccaag
    92881 ctctatacga gggttcgatt cccttcgccc gctccaattt atcttcacct catcaatctg
    92941 ccatttttgc tgtatctgat tttctatatt atcttaattc aattgcttca gacttttcgc
    93001 aatcgctgta acacttcccc atttttttgt aaataaaaag cgtttacgca atgcaatcct
    93061 tttgtaacaa cccaggtaaa ataccttatt ttcaagttgt taaataagtg gttatctacg
    93121 cttttaacaa acgattgatg gaatctttta tgaaagggaa atctacatta actcttctgc
    93181 tcgctggtat attttcgtgc ggcacatgcc aggcgacagg ggcagaagtg acgagtgaat
    93241 ctgtatttaa cattcttaat tccacggggg cagcaactga taaaagttat ttatcgttaa
    93301 atccagataa gtatcctaat tatcgtcttc tgattcactc agccaaatta aaaaatgaaa
    93361 taaagagcca ctataccaaa gacgaaattc aaggattgtt aacattaaca gaaaatacac
    93421 gaaagttaac gttgacggag aaaccctggg gaacatttat tcttgcttct actttcgaag
    93481 atgacaaaac agcggcagaa acccactatg atgctgtctg gctgagggat agtttatggg
    93541 gctatatggc attagtatca gatcagggaa atagtgtagc agcaaaaaag gttctgctca
    93601 ctctgtggga ttatatgtct acgcccgacc agattaagcg gatgcaggac gtcattagca
    93661 acccaaaacg actggatggt attcctgggc aaatgaatgc tgtacatatc cgatttgaca
    93721 gcaactcccc cgtgatggca gatgtgcagg aagaaggcaa gccgcagctg tggaaccata
    93781 aacaaaatga tgcgttaggt ctttatcttg atctccttat acaagcaatc gacactggta
    93841 ctattaacgc tgaagattgg caaaaaggtg accgattaaa atcggttgcg ctgctgattg
    93901 cttatctgga taaagccaat ttctatatta tggaagattc cggagcgtgg gaagaagatg
    93961 cccgattaaa cacatcttcc gttgcactgg tgacctctgg actggaacgt ttgtcaaacc
    94021 tactctcgaa gaaagactct gtttttgtct ctgatttact acgcgaagct aaagccaatg
    94081 aactggatgc aactttttca acaacgaggt tgaaccacct catagataaa ggttatgaaa
    94141 gaataacgtt acagcttgat ttagggggag aatccccagg ttatctggaa aaagataaac
    94201 attacagaga agccgatgcg gctttactta atgttattta tcctgcaaat ctttcaaaga
    94261 taaataccag aagaaaagag caagttctaa aaatagttaa aaaactagca ggtccgtatg
    94321 gtataaaaag atacgagaag gataattatc agtcggcaaa tttttggttc aatgatatta
    94381 aaaccgatac cgatcaaaat tcacatgcga aaagagaaat gagcttcatt ccatcaacag
    94441 aagcagaatg gttttttgac tcatggtatg caaaatcagc agcaattgtt tataaagaat
    94501 caagaaaaga agagtatttg aatgactctg tgcaatttat gaatagatca ttggcccaaa
    94561 taacaggcga gaatatgatt ggtgcaaatg gcaggagtgt acctgaaatg gcacttcccg
    94621 aaagctataa ctatatccac aagtcgggaa ctttgcatga agcgccaagc cctatcattc
    94681 cgctaaattg gtcgaaagct tcaatgactc tcatgttgaa agagatgtcc aaccttatca
    94741 atgatgaggg aaataaatag ttttcataat gaggcggtcc tggattaaaa ggaccgctca
    94801 aagagaaatc gaaaggggag gtaaaagtga tcaataaatt actgttagct tacctgatag
    94861 gtctggttgt gacatcgacc ttaatattta tcttctcgga agaaaaagta acatatcggt
    94921 tgtttgctgc agcaattaca gggttgacct ggccgcttag cctcattcca tccatcataa
    94981 gtctcatgat tcgtaaatcc gactagcaag aaaattatat gatcgattaa gaaaaataga
    95041 atcgaacaag agaataaaat atatgcgggg agtaacaaaa cactcgcagt gcgttaatga
    95101 agaactaaca aatatgattt gtattgaaaa tagttatcaa acaagtgtta taagaaatac
    95161 ggggtatgtt taagtaaaac atatcaacag ttgcaattct gtatatggac aggatgactt
    95221 actactgtaa gtagttgata gctattcgct cataacaaaa tcattatttt ataaatagga
    95281 atcatcactt tttaatcatg tttttatagc aagtttatgg agaatgagaa tatggaataa
    95341 ggagatgaaa catgattgaa gaagggctgt tacttccaat gaatgttttg cttcaaggac
    95401 agaagttaac cctactggac cctgaaatgt ggttcttacg gggaaatgaa aacaaggatg
    95461 taacccttgt tattacgatc ggtaataacc atcaaaaatt aatggtagtg gaagatatat
    95521 tactgctcat tgaccgaagc caaattgagg taactgcagg gaaagtaatt tatcatccac
    95581 ttcgcattga tatcctgagt aaattattag cttttataga tgaatcaacg gggagtgtgg
    95641 aaagggagca ccatgagttg tttgctgatg ccttgccctt tgcatcagaa gttgtgctat
    95701 tcagtaaagt tgcaagtgaa gcctggtttt tagccactta tctgagcagc gataatatta
    95761 atgaaattct gtttcagcat ctaagaaaaa cagaatgcta taaactggtt cgctatttat
    95821 tatcgcaatc gctcatacag acttcactct atgatcttgg tgagctttac ggcgtatcgt
    95881 attcacattt tcgccgttta tgtagttatg cattgggtgg taaagttaaa acggaattat
    95941 gtggctggag agttgccaga gccgtgttgg aaatcattga aggaaacagt gatatgacaa
    96001 ctattgcgca taaatatggc tactcatcct catcacactt ttcagccgaa gtaaaaagcc
    96061 ggttaggaaa aacaccgaga gaactatgta aaaagttatg aaaataaaat tacgtattac
    96121 tattatatta atttcagcct tatgcatttt taatggatta ttgactcctg gtgcatatgc
    96181 cgcaacagcg aatggatacg tagcaaataa agaaaatcta cgtagttttt ttgaaacggt
    96241 ttcatcatat gcgggtaagc ctacaatagt aagtaagctt gccatgaaga aacagatcag
    96301 cggaaatttt gatttaaccg aaccctatgc cttgattgaa cgcctgtcgg cgcagatggg
    96361 acttatttgg tacgatgatg gcaaagctat ttatatctat gactcatcag agatgcgtaa
    96421 tgcgctgatt aatctgagaa aggtatcgac gaatgaattt aataatttct taaaaaaatc
    96481 gggtctgtat aactctcgtt atgaaattaa agggggcggc aatggtactt tctatgtttc
    96541 agggccaccg gtgtatgttg acctggtcgt taatgctgca aaattgatgg agcaaaacag
    96601 cgatggcatt gagatcggtc gcaataaagt aggaataatt catctggtca acacattcgt
    96661 taatgaccgg acttatgagt tacgtggcga aaagatagtt attccgggca tggcaaaagt
    96721 actatcgaca ttgttaaata ataacattaa acaaagcaca ggagtgaacg tactttccga
    96781 aatttcaagt cgtcaacaac taaaaaacgt atcacgtatg cctccttttc ctggtgctga
    96841 agaagatgat gacctacaag tagagaaaat aatatcgaca gctggagcgc ctgagactga
    96901 caatatacaa attattgcat atcctgatac caacagttta cttgttaaag gaacagtatc
    96961 tcaggttgat tttatcgaga agttagtagc tacgctcgat attccaaagc gtcacattga
    97021 attatcttta tggataatag atattgataa gactgatcta gaacagttag gagctgactg
    97081 gtcgggcacg ataaaaattg gatccagctt aagtgcatca tttaataatt caggttcaat
    97141 tagtaccctg gatgggacac agtttattgc aacaatacag gcacttgcgc aaaaaagaag
    97201 agccgccgtt gttgctcgtc cagtcgtttt aacacaagag aatattcctg ctatatttga
    97261 taataaccgc actttctata ctaaattagt cggcgaacgt actgcggaat tggatgaagt
    97321 aacctatggt acgatgatta gcgtgctacc acgatttgct gcgcgaaatc aaattgaatt
    97381 actcctgaat attgaagatg gcaatgaaat caattccgac aaaaccaatg ttgatgatct
    97441 gcctcaggtt ggtagaacgc ttataagtac tattgcacgc gtaccccaag ggaaaagtct
    97501 tttgattggg ggatatacac gagatacgaa tacctatgaa agtcgcaaga tcccaatatt
    97561 aggcagtatt ccatttattg gtaaattatt tggatatgaa ggaacaaatg cgaataacat
    97621 cgttcgtgtc tttcttattg agccaagaga gatcgatgag cgcatgatga ataatgcgaa
    97681 tgaggctgcg gttgacgcca gagcaataac acagcaaatg gcaaaaaata aagaaatcaa
    97741 cgatgaatta ctgcagaaat ggataaaaac ttacctgaat cgtgaagtcg tcggaggata
    97801 gtgaaatggc gattcatgta gagcatgtcg gtgtattaga aagagccaga gaagtttctc
    97861 gcttagagga cattatcaca gaagataatg aagatattga agctgaaatg cctaaaatgg
    97921 gggatgatcc cgccggtaaa gaagctcgat tcttacaagc tactgacgag atgtcagcag
    97981 cgttaacgca gtttatgaag aaaaaaatct acgaagaaca actggccaac tttctggacg
    98041 gagaagaata tgtcctggaa gatcagccaa tagaaaaaac cgataaagtc atggaggcgc
    98101 ttaaggcagc aactacccat gactatgaag tgtatagctt tgctaaaaaa ttatttccag
    98161 atgaaagcga tctggtggtt gtattacgtg caattttacg taaaaaacaa atatctgaaa
    98221 atgtgcggct aaacgctgaa gcattactta gaaaagttaa tcaagagact actaaaaaat
    98281 tcattaatag cggtattaac tcggcattaa aagcaaagtt atttggccag gcgttgtcat
    98341 taaatccaaa attactaagg gcaagttatc gacaattttt aatggcagaa gacgatgctg
    98401 tagatactta tgtagaatgg attggtagtt atggctatca aaatagaatg ctagtgacaa
    98461 aatttatcaa agagacgctt ttctcagata taaatgcact tgatgcgagt tgttcatcat
    98521 tggaatttgg tatgtttctc aataagttgt cacaactatt gtctcttcag tcggcagagg
    98581 ctttatttct gaaaacttta atgaataacc caataattaa aaaatttatt agcgcagaag
    98641 attactggat attttttctg atatcgttaa taaaatttcc tgagactgca gaggaactcc
    98701 tgaaaaatgc actggtaacc ctacccgcag atgctaatta taaagacaaa acgttactcc
    98761 tgaaggcgat ttacagcgga tgtacaaatc ttccattttc actttttatt aataatgaac
    98821 aattattaga gattagggag tgttgtaaac aggcaataaa agtaacattt gcagtagagc
    98881 tctttgatac acaaaatagt aataaaaaac aaaataaaaa accatggaag aaggtaatgt
    98941 tcaatgttta acaaagtttt gatagggtta aggagtcatc cagaattaat aattcttgga
    99001 ttaatggtga tgattatcgc catgctcata attccattgc ccacctactt aatcgatttt
    99061 ttgattgggc tgaacttaac attggcaata cttgtatttt taggatcatt ctatgttgat
    99121 aggatcttaa gtttttcttc attcccatca attcttctca tcacaacgtt atttcgtttg
    99181 gcgctggcga tcagtaccag ccgacttata cttcttgagg cggacgccgg agaaattata
    99241 acgagttttg gtgaattcgt tattggcgat agcctggtag ttggttttgt tattttctcc
    99301 attgttacta ttgttcagtt tatcgtgatt actaaaggtt cggaacgtgt ggctgaagtt
    99361 gctgcccgct tttcgcttga cggtatgcca ggtaaacaaa tgagtattga cgccgatcta
    99421 cgcgccggga tcattgatgc agatttagca aaagaacgtc gtagcgtatt agaacgagag
    99481 agtcagctgt atggttcctt tgatggtgcg atgaaattta tcaaaggtga tgctattgca
    99541 aacattatta ttatctttgt taatattatc ggtgggctat cagtaggagt aggccaaaat
    99601 ggaatggatt tctcgacagc gttgaccgtc tacacaattc ttaccgtcgg tgatggtctt
    99661 gtttctcaaa ttccagcttt attaattgct attagcgcag ggtttatcgt aacgcgcgtg
    99721 aatggcgata gtgataatat ggggcaaaat ataatgtccc agttattaag taattctttt
    99781 gtcattattg tgacatgtgt cctcgcgcta agcattgggt tgcttccagg ttttcccctt
    99841 cttgtgtttt tatgtttggc agtaatctta ggaatttatt tttactttaa attcaaaaaa
    99901 aaaggggctg aagaaacggt tgttgaggga gatatcaccg ttgggttgga accctataat
    99961 caggatgatg atatttcatt aggtattatt aataaacttg atcaggttat taccgaaacg
   100021 gtgcccttgg ttttgattat gaatagtgta caggctaaaa aatacactga aatcaatctt
   100081 gctgatagga ttcgtagtca gttttttatt gagtatggta tccgtattcc cggtattgtc
   100141 attcgcgagg gggaaggcct taatgatgaa gatgtcatat taatgctaaa tgaagtcaga
   100201 gcctcgcaat ttaaaattta tcatgatctc gtcttgttgg ttgaatattc agatgaagtg
   100261 gtttcgactt tgatcaagaa acctgtcatc gtaaatagta atggtgaaca atattattgg
   100321 gttacaaaaa gtgatgccca aaaattaaca aaaattggct gttatactcg cacggccatg
   100381 gatgaaatgt acaatcattt atctgtatgt ctggcgcata atattaatga gtattttggt
   100441 atacaagaaa ccaaatatat tcttgatcaa ttagagatga agtatcctga tctattaaaa
   100501 gaaatattaa gatatataac cgtgcagcgc atttcagagg taattcagcg tctgatccag
   100561 gagcgaattt cagtgcgcaa tatgcgttta gttatggagg ctttagcatt atggagccca
   100621 cgagagaaag atataattac actcgtcgag catgtacgag gtgcattagg tcgctacatt
   100681 tgccataagt tttcatattc tggagaaatt aaagcaattg tgatttcgcc tgaaatagag
   100741 gacagaatca gagatggtgt caggcctacc gctggcggta cttttcttaa tctcgatgca
   100801 tccgaagctg agatgattct ggataatttt aaactggctc tttctggaat caatattcct
   100861 attaaagaca ttattttact gggatctgtg gatattcgtc gttttatcaa aaaacttatt
   100921 gaatctagtt atcgagacct cgaagtactt tcgtacgggg aactaacgga aaatgttccc
   100981 gttaatattc tgaaaactat ttaggataat gtcttgcgat atggagcatg ctggtatgaa
   101041 aaaattaaaa ctattaaata aatacagcta cttacatagc ataaacggct cgctgattga
   101101 agcagagctt gacgacgttt cagtgggcga agtatgcgaa atttatgcga gctggcaagc
   101161 aaatgaacgt atagccaggg ctcaggtcgt tggctttcgc aatggaaaga cattattaaa
   101221 ccttataggc agctctgtgg ggcttacacg tactgcagtt ttgaaaccaa cgggagagca
   101281 gttaaccata cagatcagtg atgcctttct tggttcagtt ttgaatgctt ctggtcaaat
   101341 tatggaaagg tttgtcccgg acactccagg cgaaagggga aatttacgcc tgattgatga
   101401 actgccaccg tcgtatcagg aacgtcgagt tattaataca ccactagaga cacggataag
   101461 agtcattgat ggcgtactga catgtggcat tggtcaacgt gttgggattt tcgcatcggc
   101521 tggctgtggc aaaacggttt taatgcatat gcttgtaaac aacactgaag ctgatgtgtt
   101581 tgttattggc ttgattggcg aacgtggaag ggaagttacg gaatgcgcgg aatcgctgaa
   101641 aaaatcagta aatgcagcaa aatgtgtttt ggtttatgcc acctctgatt tttcgtcagt
   101701 agatcggtgt aatgccgccc tgatggcgac aaccgtggca gagtatttta gagatcgagg
   101761 aaagagagtt gtacttttta tcgactcaat gacgcggtat gcacgagcac tacgtgatat
   101821 gaaactggca gcgggtgaac cacctgccag acgtggttat cctgcttctg tttttgacag
   101881 tttgcctcgc ttactggaga ggccgggacc aacactaaaa ggaagtatta ctgcctttta
   101941 taccgtatta ctggaggggg aggatgaatc agacccactg ggggatgaaa ttcgctcaat
   102001 ccttgatggc catatctatc taagtcgcaa actcgctggg caaggtcatt atccggcaat
   102061 agatgttcta aaaagtgtga gtcgagtatt tggtcaggtc actgatgaaa aacacagaga
   102121 taatgctgca cgggtaagga agatcctcac aactttggaa gatctacagg tgtttattga
   102181 tttaggggaa tatcgagctg ggcaaaatgc tgaaaatgat tttgctatga atgcacggcc
   102241 taaactcaca aattggttga aacagtcagt taatgaaaaa atgccaatga gtgaaacgct
   102301 aaaagagctt gaacgaattg ttaagtaaag ttaatcgact tataagaaga acagcgcaga
   102361 gcctggctgc atgtgaagca tcattacaaa agttaaatgc agaaaaagaa aagttagccg
   102421 aaaaggaacg cctgtatgat atgcaactta aaaacctgca gtcactgtta gatatgaaag
   102481 agttactcgg cgaagttgta tttcgacagg atatatttta ctcgttacgt aaagttgcag
   102541 tcattcagca gcagatcgct gagataaatc ttgaaaagca aaaaatagca gagcggcgaa
   102601 aaatacttaa caaggaaata gttcaacagc aagcacaaag aaaacactgg tggctaaaag
   102661 gagaaaagta cgatcgacta aagaagcgca tcaaaaaaca acttttaaat cagatgcttt
   102721 atcaagatga gcttgaacag gaggaaaaat ataatggacg aagtcaagaa aattgaacat
   102781 tataaaaatt taaacacaga cacatccgag aattctgctg cggatttaga cagtttctta
   102841 aagaaaaaca aaaaaaataa agaaatgagt gagattgttg ctggatccat tctccagtta
   102901 ttacgtcagg ctcaggatag ccaattccct acgtttggga aaaagaatgg gcaaatgaag
   102961 tctattgata acaaatcgtc tctccaggtt gacaggaatt catctttttc tttgcaaaca
   103021 aatggaatag agcaactttc tacaaagaaa gtcctcaata tgacttcacg atatcagggt
   103081 gttaattctg aactatctga taaagcgata caatttaagg gggaaatcca taatggtatc
   103141 catacagaaa acatcacggt tcaaaaccac agtaataata aagagcgtga aaaaaaatat
   103201 cgcgatggag acaaaaaaaa tggagagcag gcccatttat tagatattac taacgaaaga
   103261 aggtttgctg ataacaaaat catgtttact caacatatcg aaaagcaaag gaatgttcat
   103321 acgctcaatc agaatgatat aaataattct gttaatagtg ctaatgtacc cgaaaatgaa
   103381 ttgacatatc agtttcagcg ttggggacag aatcatacag ttcggattct ggaaagtagt
   103441 gaagggatac gactaaaacc atcagacacg attgtctccg acagactcca tgaagcccaa
   103501 cacaatgatg ttacagcaca acgttgggta ttgactgagc aagatgaacg acaagggcaa
   103561 cgacatcagc ctcatgatga gcaagaaaat gaaggtaaat tcgaaaacga tcagaaggac
   103621 gaaagttaat gttcggtctg agaaaagtaa atggaaacac tcattccttt gaaaccactt
   103681 ttctaaactg gaaagaaaag ggtgaagatg ttgcgttatt aatgcccgaa tttagcgcca
   103741 aatggctacc gatagcagaa gagagcggca gctggtctgg atgggtgtta cttcgagagt
   103801 tatttccctt aatttctgcc gagctcgctg ggatggcttt aatgccagaa acggaaaggt
   103861 taatagtgga atggctcagt ttatctagta ccccgttaaa tctaaagtat cctgaactaa
   103921 aatataatcg tttgtgtgtc ggtaaagtat ttgatggagt attgagtcca gctcaaccac
   103981 taataagaat atggacaggg gaattaaatg tttggttaga taaagtcacg gtctgccaat
   104041 acgaaaacgc tccaacacta gacaaaaaaa cattatattg gcccattcat tttgtaattg
   104101 gatttagtaa atcatgttat agaaccattg ttgatattga agttggtgat gttttattaa
   104161 tttcagataa ttttgcttat gcggttattt ataatacaaa gatttgtgat ctgatttatc
   104221 cagaggagtt aaaaatggct gatcattttg agtacgagga agattttgaa acagatgact
   104281 ttgatatcaa aaaaagcgaa atcgagatta atgatgaaaa tgactatcag cagattaata
   104341 gttttgaaga cttgcctgta aaaattgagt ttgttcttgg aaagaaaata atgaatctat
   104401 atgaaataga cgatctctgt gcaaaaagaa ttataccttt gcttcctgaa gctgagaaaa
   104461 atatagagat acgtgtaaat ggcgcgctaa ctggctatgg cgaacttgtt gaagtggatg
   104521 ataaattggg cgtagaaatt cattcttggt tatcagggaa taataatgtc aaatagcatt
   104581 tcgctgatag cgatattatc attatttaca ctattaccgt ttataatagc atccgggaca
   104641 tgttttatta aattctcgat tgtttttgtt attgtacgaa acgctttggg acttcagcaa
   104701 gtgccatcaa atatgactct taatggggtg gctttgctat tgtcaatgtt cgtaatgatg
   104761 ccggttggga aggaaatata taataacagt cagaatgaga atctttcatt caataatgta
   104821 gcgtcggttg ttaattttgt tgagacaggc atgagtggtt ataaatcata tttgataaaa
   104881 tattctgaac cggagttggt tagctttttt gaaaagattc agaaggtgaa tagttctgaa
   104941 gacaatgaag agataatcga tgatgataat atttcaattt tctcgttact cccggcatat
   105001 gcgttgagtg agataaaaag tgcatttatt ataggtttct atatctattt accctttgtt
   105061 gtagtggatc tggttatatc cagcgttctg ttaacgttag gtatgatgat gatgagtccg
   105121 gtaaccatat ctacaccgat aaaattaatt ctgtttgtcg cgatggacgg ttggacaatg
   105181 ttatcgaaag gactcatttt acaatatttt gatctatcca ttaatccctg aggaaaaaat
   105241 atggacgaca ttgtttttgc ggggaatagg gcgctgtatc tgattttagt tatgtctgca
   105301 ggacctattg cagttgcgac atttgttggt ttattggtag ggctttttca aacggtaaca
   105361 cagttgcaag aacagacgtt gccatttggc gtcaaattat tgtgcgtaag tatttgcttt
   105421 tttttaatga gcggatggta cggtgaaaaa ttatactctt ttggtattga aatgctgaat
   105481 ttagcattcg cgagaggata gcatggggga ggcgatactc tatcagttgc atagtctgct
   105541 ggctgctaca gcgctcggtt tttgccgatt ggcgccaact ttctatttgt taccattttt
   105601 cgccagtggg aatataccaa cagtagttcg tcacccaata ataatagtgg tatcatgtgc
   105661 attagttcag cattaccact atgaattatc gaccttaaat gaaatagata tcgctttgtt
   105721 agctgccaga gaaattatca tagggctgtt tattgcttgc ttattagcat ccccattttg
   105781 gattttcctt gctataggca gctttatcga taaccaacgt ggtgcaacgt taagtagtac
   105841 tttggatccg gctacaggtg tcgatacatc ggaattagca agattattta accttttctc
   105901 tgcagcagtt tatcttacta acggtggatt gaactttatt ctggagactc tatggcaaag
   105961 ttataactta tggccttcag gaaattttaa ttttcctaaa ttagagcctt tattctcata
   106021 tattaataat ataatgactc atactatcgt ttacgcaagc cccgtgattg cggttatgct
   106081 gggcggtgaa gctgtcctgg gattgctggc acgctatgct tcacaactaa atgcttttgc
   106141 catctcactg actgtcaaaa gtgcactggc atttttaata ttaattattt attttggccc
   106201 aatactggct gaacgagtta tgccgttatc ctttttccca gaacaattgc aattatacat
   106261 tgataaatag agtgtttcat ggcaaataaa actgaaaagc caacacaaaa gaaactccag
   106321 gatgcttcta aaaaagggca aattctaaaa agtagagact taacagtctc tgtaataatg
   106381 cttgtgggta ctttatatct cggatatgta tttgatgtgc atcacattat gtcgattctt
   106441 gaatacatcc ttgatcataa cgctaaaccg gatatttggg actattttaa agctatgggg
   106501 ataggttggt tgaaaacgat cattcctttt ttgctggttt gcatgtttac aacaatactt
   106561 gtctcatggt ttcaaagtaa aatgcaatta gcaactgaag ctattaagtt taagtttgat
   106621 tcattaaatc cagtaaatgg cttaaaacgt atatttggct taaaaaccgt aaaagaattt
   106681 gttaaagcaa tactttatat tatttttttt gcattggcga tcaaagtatt ttggactaac
   106741 cataaatcac tgctttttaa aactcttgat ggagatatca tttctttatt atcagattgg
   106801 ggagagatgc tattccttct catactatat tgtctcggca gtatgataat tgttctaata
   106861 tttgatttta tcgctgagta ttttttattt atgaaagata tgaaaatgga taaacaagaa
   106921 gttaaaagag aatacaagga acaagaagga aatccagaaa ttaagtctaa acgcagagag
   106981 cgccatcaag aaattctgtc agagcaattg aaatctgatg tcagtaatag ccgtctgatg
   107041 attgccaacc ctacgcacat tgccataggg atatacttta agccacatct gtcacctatc
   107101 ccattgattt ctgtaagaga aactaatgag gtagcattag ccgtaaggaa atatgcaaag
   107161 gaaatcggaa taccaattat tacagataaa aaattagcac gaaaaattta tgctacccat
   107221 cgtcgctacg attatgttag cttcgaaaat atagatgaga tcttgcgtct tctgctgtgg
   107281 cttgaagatg tggagaatgc tggacaacct gttccagatg aactgctccc ctcagaagat
   107341 aaattcaaag aaggtgaaga cacaaaaagc gagaataaag acaataattt aaaaaattaa
   107401 acatctatca tagtcagaaa tttataagaa tctacacgca aaaagcgtgt agattcttat
   107461 ttaatgagtt aaacgatctt gcgaaatgac aagcaattga tacatactca tctctttata
   107521 agctggaaca gtagtaacct gtgcaaattc gcgatatagc ttctgctcta gttctgctga
   107581 tgatatatat tccgggtgtt cgctaaagaa ctgtcttgca tctactatcg cttgagattc
   107641 gccacttctt ttattctgag tatcctttaa tgaattttct ctggtgcatg cagagagcaa
   107701 cgatatcagc gcgacagtta ttatgatttt actattatat cttactaatg acatatcaac
   107761 tttctcttat gcaagattgg agtttaaaat aatgtttatc ccattcacag agtgctataa
   107821 acaactcaac agtacctttt atgttaagtt tatttagggc tcttattttg tgatatgata
   107881 ctgttttata atcaattcct ttgataatgg ctatttgttt taacgaataa gaaacacata
   107941 accgcaggat atctatctca tcatgggtaa gtggttcttg agtggaatac acattttgtg
   108001 tatgaagatt cgaaaccagg aaatgcgtta aatcagataa tgtaatattc ttattgatcc
   108061 tgatgaaatt gttgctcaaa agggtgttta caggttctgt atttggtttg agatttcgat
   108121 gttggtgctc atcaataaca acgaacgaat gtccaagatc aaccaacgca accaaatcgc
   108181 tgtaatattc cttttgggat gctaataatg cattagtgcc tacagtgaag tagacatcac
   108241 tggaaaagac ttgcatatgc caacctgcac tctaaaatca tttagggtaa atattacgtg
   108301 taaaatttaa aataatggta attgttatat ttgcatatca atgagggaat agggtgtata
   108361 ccttaaattg caaataaagt tatgtatgta gccatttaaa atggctctcg aaaattataa
   108421 tctttttatt gtgtgtcatc ataatttggt catgaaattt ccgtcttgtt acactactct
   108481 aaactagagc ttatggaatt agcgggtggt attcatggaa aataatgata aattcttatc
   108541 acaagactta ttggaatctt atgccattcg tttattgagc ggaccattga atggatgcga
   108601 gtatgaaata cttaatgggc gtgtactcgt tatcgttggt aatgatgttt cgttaggaag
   108661 aagtgattct ttttctgagt taccagagaa tacgatagtc attccttatg gagagcttac
   108721 aggcagtttt gaggtaataa tcactaccga ccctgatctg gttgtaacaa tcagagaatt
   108781 aacagcacaa gaacctgaat atagaacgtt aacattgaat cagcaaatag aagttttagg
   108841 cctcaaattt gctgtaaaag gaaaaaatga agtttggcag tattcaatac caggtattgt
   108901 tgaaaataac attacttcaa caaggcaaca tttctttagt agtacgctgt ttaaatatgt
   108961 aacgctattt tttctttttg ctatcatttt tatagctttt tatattgtta atgccagtaa
   109021 tgatccgcag ctgagacata tcgataaaat tcttgtaaac aaaaatagaa attatgaaat
   109081 tttatatggt agggatcatg tcatctatat caataccaat agtttggatg aagctgtctg
   109141 ggtcaaacaa gcattggaaa aaaatcaacc aggaaaacca gtacgggtga taaatcctga
   109201 tgatgaatcg atacgaattt tttcatggct tgctgataat ttcccagatc tacaatattt
   109261 taaactacag ctattagatg ccagtaatcc aagactgaca gtgagtaagc aacggaatgc
   109321 tatcacacag caactaatcg ataatcttat taaaggatta ctgcaaacta tgccatatgc
   109381 cagcaatata agtattgcgg tattagatga taatgtattg gaaagtcagg caattgaaac
   109441 attgtcggca ataggtcttt cttatgaaaa atataaaaca gctaacaatg tatatttcaa
   109501 tatcattggt acgttaagcg acagtgaatt aaataaaatt aataattatg tcgacgaata
   109561 ttataaacaa tggggtaaac aatatgtaag atttaatgtg aatttgaaaa atcaggacac
   109621 aaataatagt tcatttagct acggagataa tcgattcgag aagtctcaag gtagcaagtg
   109681 gacatttcag taataattga ggaggactta tggcagattg gaatggttat attatggata
   109741 tcagcaaaca atttgatcag ggggttgatg atctgaacca gcaagttgaa aaagcgttgg
   109801 aggatttagc aaccaatcct tccgacccga aattccttgc cgaatatcag agtgcattag
   109861 ctgagtatac attatatcga aatgcgcaat ctaacgttgt aaaagcgtat aaagatcttg
   109921 attcggcaat catacaaaac ttccgctaaa ctggataagg attttattat gtcagtaagc
   109981 aatatgccac ctattgatag agctgagcaa tcagctgctc atgaaataca gcaagcgaaa
   110041 gttattgatt taaatgaccg ggttctcaat cttgataacc cggatgataa aatgatctcc
   110101 gcttttgcta actatgcggt acaaactgaa aattggcagc aaaatgcgtt acaagcatta
   110161 aggtctgata aaaaggggtt aactcctgaa aaattattag tcttgcagga tcatgtgctt
   110221 aattataatg tcgaggtttc attggtggga acattagcaa gaaaaattgt cgctgcagtt
   110281 gagacgttga ctcgctcatg aaatatatat cattattctt gtttatcctg ctgttgtgcg
   110341 gttgcaagca gcaggaatta ctgaagcatc ttgatcaaca gcaagctaat gatgtccttg
   110401 ctgttttaca aagacataat attaatgctg agaagaagga tcaaggcaaa attggatttt
   110461 caatctatgt agaaccaacg gattttgctt ctgcggtgga ttggctaaaa atctacaatc
   110521 tcccgggtaa gcctgatatt caaatatcgc agatgtttcc tgcagatgca ctcgtttcct
   110581 ctccacgagc ggaaaaagca agattgtatt ctgctattga acagcgttta gagcaatctc
   110641 tcaaaattat ggatggcatt gtttcttcaa gagtacatgt tagttacgat gttgatactg
   110701 gtgatagtgg caaaactgct ttaccaattc atatttctgt acttgctgta tatgaaaaag
   110761 atatcaatcc agaaataaaa ataaatgaca ttaagagatt tattgttaat agctttgcaa
   110821 gcgtacaata tgagaatatt tcagtcgtat tgtcaaaacg aagagatatt attgaacaag
   110881 caccaacata tgaaattaac gaacctattt ttgcttatga taagactatg ccggtttcta
   110941 tcttacttgc attaatgtcc attgcgactt gctggctgtt atggaagtac agggctattc
   111001 taactaattt aatcaggtta aaaaataagt agacatatga gaacgttatg aatcttgccc
   111061 taagaaagat tatatatgat ccaattagtt atattcatcc tcaacgagta agttggaata
   111121 atgcacgtat tagcaatccg gtacttagat ctattaccaa tgaaatgata ctcctacagt
   111181 acaatctttc agttgagcat tttagtctga atagttcact tatttactac ataaacaatt
   111241 ggaaattatt tccattgatc tgtttactca gtggttgtca cttttatcga gaaaggtttg
   111301 cagaacgcgg atttttttac aaggttcctg atgtgctaag gaattattta tcagcgatac
   111361 cggtggaaat aaatgaaaag gcacgatata aacttggcat cgttaactat cagaatatca
   111421 tcacatgtgg tttttcaacg ttgttgccct atgtaaggca acaaccgttg gcaatgcagc
   111481 aacgatttaa tttattattt cctgattttg ttgaccacat acaattgccg ttaccagttg
   111541 cgtctactct tttggagaga attacatttt atgcgaaaaa aaatagagat gagcttgata
   111601 aactctcctg caaatggtgt tgtgattaaa agaataattt ctgatggatt gaaagaaata
   111661 gtttctctaa aagaacagat ccttcttgaa accaccgcaa aaattcagag tattgaggaa
   111721 aagagagaag aaaaaattat tcaaggttat tatgatggct atgcgaaagg cattattgat
   111781 gtgatggata attttatacc cttgataagt ttgctgagct cagaattaga aaaaaacaga
   111841 gtaaacatga taaacgatct gaagagcata ttgttaaaat cttcagagga agttgaagtt
   111901 tttatcaaaa tttttgaaag ttgggtaaca aaattaccgt caatatcagg ccctttaaat
   111961 ctctatatac ctaccagttt caaagataag tctattgaag tagagtcata ttttgtcgac
   112021 aagtcaatat ggaatgtcca tatttcatat catgatgaca aacgttttat tttttttaca
   112081 gatcaattta ttgctgaatt tagtccgcag gaatttgtgg ataattgtga gcaatattta
   112141 attagcaatc actgtttttc ccctgacaag gttaatgaaa tctgtgaaca tgccagacat
   112201 tatttagttg aaagaatgtg tgaaatagat tctttggcta tgaataattc tgatttgacg
   112261 aacccagagg acttatagat tactgtctcc ggtttaataa cttgaatata atctaattct
   112321 aatattttaa acgcctaatg atttttcaac actggtgtta ttctgcttac gcgaaaattt
   112381 tatttttaat tatatttcat tattatttca tcaatgtatt ctttggattt tgcttataat
   112441 aataatggaa gggatgatta taaatgaatg cgttattgca tttacactca ggatgaatat
   112501 atgggaaaga ttaaaattgt agtttcagat cagcagccgt ttatgattga tgggataatt
   112561 ggatttctcg gacattatcc cgatttatat gaggttgttg ggggctataa agatctgaag
   112621 aaagctatag cggagtgtaa caaatctgca gcccaaatat ttattttagg agaatttgca
   112681 ggtggaatga tggggtccga actcataaaa tgggttaaat cgcataaaat agatgctcat
   112741 attattacat ttgtagcaaa aatgccgtat attgattcaa taaaattgct tgaagccggt
   112801 gcgaaaggat gcgtatggaa aaccagtcac ccggcgaaac taaatcgcgc tattgattcg
   112861 attagtaatg gttatactta ttttgatagt gtacatatgg attgtgaaaa aatatcttca
   112921 agatattcct ctgataatca acttacaaat cgtgagtctg aaatactaca actcatagct
   112981 gatggaaaaa caaacaaaga aatcgctaat ttcttgcagt taagcaggaa aacagtcgaa
   113041 actcatagac tcaatatcat gaagaaatta gatgttcaca gtggtatcga gttgatcaaa
   113101 acagctttac gtatgggtgt ttgcactata taaaaaaaga ggctttaggc tcattttgct
   113161 aaagccttac gcttgtctat ttcattaagt ctggtatata caacatatat tttttttgca
   113221 tattgcagcc tctttttttg gttttccatt gcaaaacccg cgttatatgt ccccagacat
   113281 tgccacgtta ccccacaacg tgaaaaatga ttatataaaa tctcggtccc tattttaatg
   113341 ttcagacagg ggttatctaa caaatctcgt tcatctttta ttattccacg tttttttaac
   113401 atgggaatgt gcatctggtt tatctgcatt aaaccataat caaaactctt tatattgttt
   113461 ttcttatccc tgttcttgcc gatgctatca ttttttaaat tactttcaac aagagcgata
   113521 gctttgatca aatttggttc gattctgaaa attgatcctg cttcattaaa gcaagcgcta
   113581 ctggcgaaag acatttttgt aattaatata gaaaacatga agatggataa gattttaact
   113641 ttgggtaagc gcatatattt ttatgtgtag gattgaagga aaggaacgag atactaatat
   113701 attcagtatc tcgttaatca tttaagtggt caattatcta ccattccagt tcatagtaag
   113761 gagagatagg attatctttc gaatacatat cattttgaaa tgatgccatc catgttgaaa
   113821 ctataaatgc ttgtatttga tctgtatcag tagcactggt ggtgatgtgt ttatctaatg
   113881 aaacgacttc atcacccgtt gcggttgcta tatttaaact ttgagaaaaa ttattcatat
   113941 tgattggatt agtcataaag ttcccttttt tatttgtcca ggccaatagc ctatacacaa
   114001 tactttttaa tctagagtat ttgatatata ttgcaataag gtaattatct tagatgtttt
   114061 catctctgtt gtttacataa ccagttgcat acatctaaac aataaaattg cgacaataaa
   114121 gatttatcgc atacaaattc tcgttagttg ctttagtttc aaaatgttgt caattatcac
   114181 tcgatggctt tcactcgaag tttctttata gtagagtttc atgttgcttt tgagcgggtt
   114241 aggcacatac atatctacac actcttttat cacaactgct tttcgtcttc tgggttcgag
   114301 ttgtagtgat ttgtctatac aaatccttgc ctcttcaatt ctttcattat gaaaattgac
   114361 aagtgctcta tagtagtaga gagaggctat atcagttgtg tgtatcttag cctgttcaaa
   114421 taagatatga gatacttttg cctgtccaga cagaccagtt atcagtccca taatagctaa
   114481 aatttttcca tcgacattgg ttatgtctga tacataatgt aaaagctcaa atgctttttg
   114541 agcagcaagt tcaagttcat actttccatg aagtgccaaa gacatatggc attccgctag
   114601 cagacaataa cattctattt ttagcgtctg tatatcactt ttattttgta atccatcaaa
   114661 tatagccata gccctgtaaa tgctttcagg agtaaactcg taaagttctt tcttaccagc
   114721 caacagttca tctgataaat aatgattctt gtaaccttgg ttcggtttag ctaatcttac
   114781 agaacgctct ggattatgta ccgtttgaag gatattatct ataataaatt gtgatgtgtt
   114841 atgtagttca tcaacaggaa gatgattact ggcgatgagg aaaagatttt tagcgtcgat
   114901 cagttcaatg tataaagttt tcaccgcgtt attctgattt attcttcctg taacgaaata
   114961 atctggcttg aatcttctca aaaatgaatt ttgcgatatg tgatcattac aaaaatttgt
   115021 cgcagccatc ggataggtat aaaggccatc gattttttta tccgaaatgt tttgcactaa
   115081 ttcctgatta agtattaatg gatccagggt attcaatgaa gtggtgaaag gaaatatagc
   115141 tatggaatag tctgaagtgt tatcttcatt tattttagtt ttgaaaacct gtccactgaa
   115201 acgataacct ttccggtaga ttgtttctat gcaacgatca tagccaattt tttcaaaaat
   115261 gcagcgcaaa gaatagatac atcttgtcag ggactcatca cttacaataa tatttttcca
   115321 tactgattcg atgatcatat ctttcaggac aacatgacca gcggattcaa gtaatacgat
   115381 caatactcct aactctttcg gtggaatata aatctgctca tttccttgag taagaattcc
   115441 atcttccgtg agcgttaatc cctcaagaaa atgatatgag aatttatttt ctaagtccat
   115501 tagttaacct ctatgaaaaa attggtttct tgcatcctgc ataagcaata gaaattgtgg
   115561 ctattcatat ttatgtgtca ttaatgctac aggagtttac attcgacata tctatggttg
   115621 tgttcactaa tttatcttag gaattcagtg atgtaatctg aaatagcctt attcaatttt
   115681 gataaaatag attgttctct aacgcgttga aaaatagtaa tttatagtgt gttcttaact
   115741 gttttaagat gttgatttaa gaaaacataa gagaagctgg tcatagtggt taactgcact
   115801 ataaccagca attttaacca aatgcttgta atgcttagcc attatcttct gaattatcgg
   115861 ttaagagttc aatatatgcc tgcgctttgc tcttaacact ttcatcctca caattgttaa
   115921 taatatgata gaaacattgc attgcgttat cgatattttt agccccaatt tgacattgac
   115981 ccatacgata aataactgaa tagtcatcat acggggagta attgtaactt agtttgtaaa
   116041 ggtcatatgc ttgttgatat ttcttttttg actggcatac tgatgcaaaa ccttttagat
   116101 attcatgatt ttcgaacgca taaatgcaca gaaatttata ataaatttct gcgtccgtta
   116161 tattaccttt ttcatggtag tcgaatgcac atgcatatat tctatcaagg atatcaggag
   116221 tcaatccatg tagtaatgcc agattttctc cttgcgataa tgcgtgtttt agttgacttg
   116281 cccattcatc actgttattg aatatttcaa gtgtatctgt gctcataata ttcctctgtg
   116341 atgtgtttcg tgatgctaaa ttattattga ttgactcatt aattttttgc atttatcatt
   116401 tttgaattat gagttttaag cttgcattgc ttatgggtat ataggttcat tattagtgaa
   116461 aatactgacc actataaacc aataatgtca ttagttgttt tgctaatatt caccttatta
   116521 gttatcttaa ttacgacatt tcataaatgc cttaatgtgc atggcctgga ttcatattat
   116581 caatttccat tttttcgatg aaaattaaaa aagggatttt tgcgaagggt agtaagtcgt
   116641 ctggtttttt ttctttatta tgtctggtga cgagggggga tagaacgtcg gtgcatatag
   116701 tcgagaattt attcaggtct aaattattct ctattttggc cagttttatg agttcatttg
   116761 tagccatact cattaaatca tctgttatcg gaaatttata tatgctcata gccgataata
   116821 ttgcttgctg aattatttct tcagaaatat ccgttatctt tattgacaaa tcatgtgtta
   116881 gatattttcc tccaagagat tcttgttcaa tataatttaa accagacctc atcgtaattt
   116941 taactggatt cacgtatttc tcttcatttt tcttattttt gttctcctga tcaggaacta
   117001 atggttgcag tggtaggcta ttattaatat ttcgtggctt cattttacat ttctctcatt
   117061 tgattagagc aaaatccgaa ttcgcagctt ctgataatgt caaatatcac cttttctcat
   117121 gctttattct atcaaaaatc gctcattttt acttttttta ccataatgtt acttagtagc
   117181 atcggcaatt tacataactt acatgttgtt ttttatatcc tgaaaaaaca gatagatagt
   117241 gtgcacactc gaaatgatat tggtaacata aatagcttta ttcgtgacaa acatctcaaa
   117301 agatttcctt tattattttc gaaattcatt tgatcaaggt tatcacctta tggagacgtt
   117361 acgtagaatg actatatttg ttatcggcac attaggggta ttaaatttag tattgatgga
   117421 aggtgattat tgaacagtaa catcgagtca cggtaaaaat gtgaggtata taatggacat
   117481 tgagttttcg cagattcatg aaatggtcta tatgcatgat attgtaaatt cggactcgaa
   117541 aaagaaacca aggattccgt taaaaaaatt ccttaatgct gagaaggttc tcacgcaaac
   117601 tggatttgcc cctatatttc cagacacctg ttatcactta acccattact ggcttgctgc
   117661 cgtagatatt cccgtggcga gcgataaccc agtgcactat gcggatgcca ttcgttataa
   117721 tgctcgaatg cctctgcaag gttctttgct gccgttaacc cgtctggttt gggcatgaca
   117781 ctgatgtagt cacgctttat cgttttcacg aagctctctg ctatgccgtt actctccgga
   117841 ctccgcaccg ccgtgttctt cggttcaagc cccaacatcc gggcaaactg ccgtgtttca
   117901 ttagcccggt agcatgaacc attatccgtc agccactcta ctggagacgc cggaagctcg
   117961 ttgccgaaac ggcgttccac cgctcccagc atgacgtcct gtactgtttc actgttgaag
   118021 ccgcccgtag tgaccgccca gtgcagtgcc tcacggtcac agcagtccag cgcgaacgtg
   118081 actcgcagtt tttctccgtt atcacagcgg aactcgaacc cgtcagagca ccatcgctga
   118141 ttactttctt tcacagccac tctgcctgta tgtgcccgtt tcgatggcgg tacagcaggt
   118201 tttcgctcaa gcaacagcgc attctggcgc atgatccggt aaacacgttt ggcattgatc
   118261 gcaggcatac catcaagttc tgcctgtctg cgaagcagcg cccatacccg acgataacca
   118321 tacgtgggca gctctccgat aacatggtgt atacggagaa gcacatccgt atcatcagtg
   118381 tgacgactgc ggcggccatc catccagtca tcggttcgtc tgagaatgac gtgcaactgc
   118441 gcacgcgaca cccggagaca acggctgact aagcttactc cccatccccg ggcaataagg
   118501 gcgcgtgcgc tatccacttt tttgcccgtc catattcaac ggcttctttg aggagttcat
   118561 tttccatcgt ttttttgccg agcaggcgct ggagttcttt aatctgcttc atggcagcag
   118621 caagttcaga ggcaggaacg acctgttctc ctgcggccac agcagtaaga cttccctcct
   118681 ggtattgctt gcgccagaga aatagctggc tggctgccac accgtgttgc cgggctacaa
   118741 gggagaccgt cattcccggt tcaaaactct gctgaacaat tgcgatcttt tcctgtgtgg
   118801 tacgccgtct gcgtttctcc ggccctaaga catcaatcat ctgttctcca atgactagtc
   118861 taaaaactag tattaagact atcacttatt taagtgatat tggttgtctg gagattcagg
   118921 ggggcaggaa ggatgtttac gaacaaagta aggggtctgt cggatactgg gtggcgatga
   118981 tccgccgtca cagtggtgag ccgtaatgtt ccgcactgcg cggatatcct gaaacatgtt
   119041 ctgttaatgt tacctggttc agaagagata aac
//
LOCUS       DAMZFT010000002       116474 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.002, whole genome shotgun sequence.
ACCESSION   DAMZFT010000002 DAMZFT010000000
VERSION     DAMZFT010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 116474)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 116474)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..116474
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.002"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(<1..94)
                     /locus_tag="PN977_000114"
     CDS             complement(<1..94)
                     /locus_tag="PN977_000114"
                     /EC_number="4.1.3.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001641973.1"
                     /note="catalyzes the formation of
                     1,4-dihydroxy-2-naphthoate from O-succinylbenzoyl-CoA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA synthase"
                     /protein_id="HDI6040201.1"
                     /translation="MIYPDEAMLYAPVEWHDCSEGFEDIRYEKSA"
     gene            complement(109..867)
                     /gene="menH"
                     /locus_tag="PN977_000115"
     CDS             complement(109..867)
                     /gene="menH"
                     /locus_tag="PN977_000115"
                     /EC_number="4.2.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-6-hydroxy-2,
                     4-cyclohexadiene-1-carboxylate synthase"
                     /protein_id="HDI6040202.1"
                     /translation="MILHAQAKHGKPGLPWLVFLHGFSGDCHEWQEVGEVFADYSRLY
                     VDLPGHGGSAAISVDGFDDVTGLLCKTLVSYNILDFWLVGYSLGGRVAMMAACQGLAG
                     LCGVVVEGGHPGLQNAEQRTQRQRSDRQWAQRFRTEPLTAVFADWYQQPVFASLNDEQ
                     RRELVALRSNNNGATLAAMLEATSLAVQPDLRANLSARTFAFYYLCGERDSKFRALAA
                     ELAAECHVIPRAGHNAHRENPAGVIASLAQILRF"
     gene            complement(864..2534)
                     /gene="menD"
                     /locus_tag="PN977_000116"
     CDS             complement(864..2534)
                     /gene="menD"
                     /locus_tag="PN977_000116"
                     /EC_number="2.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-5-enolpyruvyl-6-hydroxy-3-
                     cyclohexene-1-carboxylic-acid synthase"
                     /protein_id="HDI6040203.1"
                     /translation="MSVSAFNRRWAAVILEALTRQGVRHICIAPGSRSTPLTLAAAEN
                     SAFIHHTHFDERGLGHLALGLAKVSKQPVAVIVTSGTAVANLYPALIEAGLTGEKLIL
                     LTADRPPELIDCGANQAIRQPGMFASHPTHSISLPRPTQDIPARWLVSTIDHALGTLH
                     AGGVHINCPFAEPLYGEMDDTGLSWQQRLGDWWQDDKPWLREAPRLESEKQRDWFFWR
                     QKRGVVVAGRMSAEEGKKVALWAQTLGWPLIGDVLSQTGQPLPCADLWLGNAKATSEL
                     QQAQIVVQLGSSLTGKRLLQWQASCEPEEYWIVDDIEGRLDPAHHRGRRLIANIADWL
                     EQHPAEKRQPWCVEIPRLAEQAMQAVIARRDAFGEAQLAHRISDYLPEQGQLFVGNSL
                     VVRLIDALSQLPAGYPVYSNRGASGIDGLLSTAAGVQRASGKPTLAIVGDLSALYDLN
                     ALALLRQVSAPLVLIVVNNNGGQIFSLLPTPKNERERFYLMPQNVHFEHAAAMFELKY
                     HRPQNWQELETALVDAWRTPTTTVIEMVVNDTDGAQTLQQLLAQVSHL"
     gene            complement(2623..3918)
                     /gene="menF"
                     /locus_tag="PN977_000117"
     CDS             complement(2623..3918)
                     /gene="menF"
                     /locus_tag="PN977_000117"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase MenF"
                     /protein_id="HDI6040204.1"
                     /translation="MQSLTTALENLLRHLSQEIPATPGIRVIDIPFPLKDAFDALNWL
                     ASQQVYPQFYWQQRNGDEEAAVLGAITRFTSLDQAQRFLRQHPEHADLRIWGLNAFDP
                     SQGNLLLPRLEWRRCGGKATLRLTLFSERSLQHDAIKAKEFIATLVSIKPLPGLHLTT
                     TREQHWPDKTGWTQLIELATKTIAEGELDKVVLARATDLHFASPVNAAAMMAASRRLN
                     LNCYHFYMAFDGENAFLGSSPERLWWRRDKALRTEALAGTVANHPDDKQAQRLGEWLM
                     ADDKNQRENMLVVEDICQRLQADTQTLDVLPPQVLRLRKVQHLRRCIWTALNKADDVI
                     CLHQLQPTAAVAGLPRDLARQFIARHEPFTREWYAGSAGYLSLQQSEFCVSLRSAKIS
                     GNVVRLYAGAGIVRGSDPEQEWQEIDNKAAGLRTLLQME"
     gene            complement(3997..4302)
                     /gene="elaB"
                     /locus_tag="PN977_000118"
     CDS             complement(3997..4302)
                     /gene="elaB"
                     /locus_tag="PN977_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein ElaB"
                     /protein_id="HDI6040205.1"
                     /translation="MSNQFGDTRIDDDLTLLSETLEEVLRSSGDPADQKYVELKARAE
                     KALDDVKKRVSQASDSYYYRAKQAVYRADDYVHEKPWQGIGVGAAVGLVLGLLLARR"
     gene            complement(4357..4818)
                     /locus_tag="PN977_000119"
     CDS             complement(4357..4818)
                     /locus_tag="PN977_000119"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDI6040206.1"
                     /translation="MIEWQDLHHSELSVSQLYALLQLRCAVFVVEQNCPYQDIDGDDL
                     TRDNRHILGWKNDELVAYARILKSDDDLEPVVIGRVIVSEALRGEKVGQQLMSKTLET
                     CAHHWPDKPVYLGAQAHLQNFYQSFGFIPVTEVYEEDGIPHIGMAREVIQA"
     gene            4883..5800
                     /gene="rbn"
                     /locus_tag="PN977_000120"
     CDS             4883..5800
                     /gene="rbn"
                     /locus_tag="PN977_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease BN"
                     /protein_id="HDI6040207.1"
                     /translation="MELIFLGTSAGVPTRTRNVTAILLNLQHPTQSGLWLFDCGEGTQ
                     HQLLHTAFNPGKLDKIFISHLHGDHLFGLPGLLCSRSMSGIVQPLTIYGPQGIREFVE
                     TALRISGSWTDYPLEIVEIGAGEIFDDGLRKVTAYPLEHPLECYGYRIEEHDKPGALN
                     AQALKAAGVPPGPLFQALKAGKTIMLEDGRQINGADYLAAPVPGKALAIFGDTGPCDA
                     ALDLAKGVNVMVHEATLDITMEAKANSRGHSSTRQAATLAREAGVGKLIITHVSSRYD
                     DKGCQHLLRECRSIFPATELANDFTVFNV"
     gene            5991..7199
                     /gene="elaD"
                     /locus_tag="PN977_000121"
     CDS             5991..7199
                     /gene="elaD"
                     /locus_tag="PN977_000121"
                     /EC_number="3.4.19.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311184.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deubiquitinase"
                     /protein_id="HDI6040208.1"
                     /translation="MVTVVSNYCQLSQTQLSQTFAEKFTVTDELLQSLKKTALSGDEE
                     SIELLHNIALGYDEFGKKAEDILYHIVRNPTNETLSIIRLIKNACLKLYNLAHTATKH
                     PLKSHDSDDLLFKKLFSPSKLMTIIGEDIPLISEKQSLSKVLLNDKNNELSDGTNFWD
                     KNRQLTTDEIACYLKKIAANAKNTQVNYPTDFYLPDSNSTYLEIALNDNIKSDPSWPK
                     AVQLFPINTGGHWILVSLQKIVNEKNNTQQIKCIIFNSLRALGHEKENSLKRIINSFN
                     SELMGEMSNNNIKVHLTEPEIIFLHADLQQYLSQSCGAFVCMSAKEVIEQRESNSDSA
                     PYTLLKNYADRFKKYSAEEQYEIDFQHRQVNRNCYLDKYGDANINDYYRDLEIKHSQP
                     QNRASGKRVS"
     gene            complement(7270..9036)
                     /locus_tag="PN977_000122"
     CDS             complement(7270..9036)
                     /locus_tag="PN977_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HDI6040209.1"
                     /translation="MRNKNIIMLLMSSLILSGCGPESENKESLQQQPSTPTDQQVLAA
                     QHAAIKEAEQRSVAAKATADAKAKALAQQEAQQYSDKQALQGRLQAAPKYQHAAREKA
                     ASQIANPGTARYQQFDDNPVKQVAQNPLATFSLDVDTGSYANVRRFLNHGQLPPPDAV
                     RVEEMVNYFPSDWVINDKSNNKEPVPASKPIPFAMRYELAPAPWNEQRTLLKVDILAK
                     DRKSEELPASNLVFLIDTSGSMISDERLPLIQSSLKLLVKELREQDNIAIVTYAGDSR
                     IALPSISGSHKAEINAAIDSLDADGSTNGGAGLELAYQQAAKGFIKGGINRILLATDG
                     DFNVGIDDPKSIESMVKKQRESGVSLSTFGVGDSNYNEAMMVRIADVGNGNYSYIDTL
                     AEAQKVLNSEMRQTLISVAKDVKAQIEFNPAWVTEYRQIGYEKRQLRAEDFNNDNVDA
                     GDIGAGKHITLLFELTLKGQKASIDKLRYAPDNKSAKSDKTKELAWLKIRWKYPQGKE
                     SQLVEFPLGPTINAPSEDMRFRAAVAAYGQKLRGSEYLDEISWQQIKQWAQQAKGEDP
                     QGYRAEFIRLVELAGGVTDISQ"
     gene            9184..10149
                     /locus_tag="PN977_000123"
     CDS             9184..10149
                     /locus_tag="PN977_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001353070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HDI6040210.1"
                     /translation="MNFAFIILILFSLPLIIFYQPWVNALPPTPRHASPEQLEKTVRY
                     LTQTVHPRSADNIDNLKRSAEYIKEVFVSSGARVTSQDAPITGGPYKNIVADYGPADG
                     PLIIIGAHYDSASSYENDQLTYTPGADDNASGVAGLLELARLLQQQAPKTGVQLVAYA
                     SEEPPFFRSDEMGSAVHAASLERPVRLMIALEMIGYYDSAPGSQDYPYPAMSWLYPDR
                     GDFIAVVGRMQDINAVRQLKAALLSSQDLSVYSMNAPGFIPGIDFSDHLNYWQHDIPA
                     VMITDTAFYRNKQYHLPGDTADRLNYQKMAQVVDGVITLLYNSKQ"
     gene            10249..10752
                     /locus_tag="PN977_000124"
     CDS             10249..10752
                     /locus_tag="PN977_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbM family protein"
                     /protein_id="HDI6040211.1"
                     /translation="MGMIGYFAEIDSEKINQLLESAEKPLMDNIHDTLSGLRRLDIDK
                     RWDFLHFGLTGTSAFDPAKNDPLSRAVLGEHSLEDGIDGFLGLTWNQELAATIDRLEA
                     LDRNVLRKQFSIQRLNEMEIYPGVTFSEELEGQLFASIMLDMEKLISAYRRILRQGNH
                     ALTVIVG"
     gene            complement(10820..12277)
                     /gene="nuoN"
                     /locus_tag="PN977_000125"
     CDS             complement(10820..12277)
                     /gene="nuoN"
                     /locus_tag="PN977_000125"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HDI6040212.1"
                     /translation="MTITPQNLIALLPLLIVGLTVVVVMLSIAWRRNHFLNATLSVIG
                     LNAALVSLWFVGQAGAMDVTPLMRVDGFAMLYTGLVLLASLATCTFAYPWLEGYNDNK
                     DEFYLLVLIAALGGILLANANHLASLFLGIELISLPLFGLVGYAFRQKRSLEASIKYT
                     ILSAAASSFLLFGMALVYAQSGDLSFVALGKNLGDGMLNEPLLLAGFGLMIVGLGFKL
                     SLVPFHLWTPDVYQGAPAPVSTFLATASKIAIFGVVMRLFLYAPVGDSEAIRVVLAII
                     AFASIIFGNLMALSQTNIKRLLGYSSISHLGYLLVALIALQTGEMSMEAVGVYLAGYL
                     FSSLGAFGVVSLMSSPYRGPDADSLFSYRGLFWHRPILAAVMTVMMLSLAGIPMTLGF
                     IGKFYVLAVGVQAHLWWLVGAVVVGSAIGLYYYLRVAVSLYLHAPEQPGRDAPSNWQY
                     SAGGIVVLISALLVLVLGVWPQPLISIVRLAMPLM"
     gene            complement(12284..13813)
                     /gene="nuoM"
                     /locus_tag="PN977_000126"
     CDS             complement(12284..13813)
                     /gene="nuoM"
                     /locus_tag="PN977_000126"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HDI6040213.1"
                     /translation="MLLPWLILIPFIGGFLCWQTERFGVKVPRWIALITMGLTLALSL
                     QLWLQGGYSLTQSAGIPQWQSEFDMPWIPRFGISIHLAIDGLSLLMVVLTGLLGVLAV
                     LCSWKEIEKYQGFFHLNLMWILGGVIGVFLAIDMFLFFFFWEMMLVPMYFLIALWGHK
                     ASDGKTRITAATKFFIYTQASGLVMLIAILALVFVHYNATGVWTFNYEELLNTPMSSG
                     VEYLLMLGFFIAFAVKMPVVPLHGWLPDAHSQAPTAGSVDLAGILLKTAAYGLLRFSL
                     PLFPNASAEFAPIAMWLGVIGIFYGAWMAFAQTDIKRLIAYTSVSHMGFVLIAIYTGS
                     QLAYQGAVIQMIAHGLSAAGLFILCGQLYERIHTRDMRMMGGLWSKMKWLPALSLFFA
                     VATLGMPGTGNFVGEFMILFGSFQVVPVITVISTFGLVFASVYSLAMLHRAYFGKAKS
                     QIASQELPGMSLRELFMILLLVVLLVLLGFYPQPILDTSHSAIGNIQQWFVNSVTTTR
                     P"
     gene            complement(13977..15818)
                     /gene="nuoL"
                     /locus_tag="PN977_000127"
     CDS             complement(13977..15818)
                     /gene="nuoL"
                     /locus_tag="PN977_000127"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HDI6040214.1"
                     /translation="MNMLALTIILPLIGFVLLAFSRGRWSENVSAIVGVGSVGLAALV
                     TAFIGVDFFANGEQAYSQPLWTWMSVGDFNIGFNLVLDGLSLTMLSVVTGVGFLIHMY
                     ASWYMRGEEGYSRFFAYTNLFIASMVVLVLADNLLLMYLGWEGVGLCSYLLIGFYYTD
                     PKNGAAAMKAFVVTRVGDVFLAFALFILYNELGTLNFREMVELAPAHFADGNNMLMWA
                     TLMLLGGAVGKSAQLPLQTWLADAMAGPTPVSALIHAATMVTAGVYLIARTHGLFLMT
                     PEVLHLVGIVGAVTLLLAGFAALVQTDIKRVLAYSTMSQIGYMFLALGVQAWDAAIFH
                     LMTHAFFKALLFLASGSVILACHHEQNIFKMGGLRKSIPLVYLCFLVGGAALSALPLV
                     TAGFFSKDEILAGAMANGHINLMVAGLVGAFMTSLYTFRMIFIVFHGKEQIHAHAVKG
                     VTHSLPLIVLLILSTFVGALIVPPLQGVLPQTTELAHGSMLTLEITSGVVAVVGILLA
                     AWLWLGKRTLVTSIANSAPGRLLGTWWYNAWGFDWLYDKVFVKPFLGIAWLLKRDPLN
                     SMMNIPAVLSRFAGKGLLLSENGYLRWYVASMSIGAVVVLALLMVLR"
     gene            complement(15815..16117)
                     /gene="nuoK"
                     /locus_tag="PN977_000128"
     CDS             complement(15815..16117)
                     /gene="nuoK"
                     /locus_tag="PN977_000128"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HDI6040215.1"
                     /translation="MIPLQHGLILAAILFVLGLTGLVIRRNLLFMLIGLEIMINASAL
                     AFVVAGSYWGQTDGQVMYILAISLAAAEASIGLALLLQLHRRRQNLNIDSVSEMRG"
     gene            complement(16114..16668)
                     /gene="nuoJ"
                     /locus_tag="PN977_000129"
     CDS             complement(16114..16668)
                     /gene="nuoJ"
                     /locus_tag="PN977_000129"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HDI6040216.1"
                     /translation="MEFAFYICGLIAILATLRVITHTNPVHALLYLIISLLAISGVFF
                     SLGAYFAGALEIIVYAGAIMVLFVFVVMMLNLGGSEIEQERQWLKPQVWIGPAILSAI
                     MLVVIVYAILGVNDQGIDGTPISAKAVGITLFGPYVLAVELASMLLLAGLVVAFHVGR
                     EERAGEVLSNRKDDSAKRKTEEHA"
     gene            complement(16680..17222)
                     /gene="nuoI"
                     /locus_tag="PN977_000130"
     CDS             complement(16680..17222)
                     /gene="nuoI"
                     /locus_tag="PN977_000130"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015959823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HDI6040217.1"
                     /translation="MTLKELLVGFGTQVRSIWMIGLHAFAKRETRMYPEEPVYLPPRY
                     RGRIVLTRDPDGEERCVACNLCAVACPVGCISLQKAETKDGRWYPEFFRINFSRCIFC
                     GLCEEACPTTAIQLTPDFEMGEYKRQDLVYEKEDLLISGPGKYPEYNFYRMAGMAIDG
                     KDKGEAENEAKPIDVKSLLP"
     gene            complement(17237..18214)
                     /gene="nuoH"
                     /locus_tag="PN977_000131"
     CDS             complement(17237..18214)
                     /gene="nuoH"
                     /locus_tag="PN977_000131"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015875065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HDI6040218.1"
                     /translation="MSWISPELIEILLTILKAVVILLVVVTCGAFMSFGERRLLGLFQ
                     NRYGPNRVGWGGSLQLVADMIKMFFKEDWIPKFSDRVIFTLAPMIAFTSLLLAFAIVP
                     VSPGWVVADLNIGILFFLMMAGLAVYAVLFAGWSSNNKYSLLGAMRASAQTLSYEVFL
                     GLSLMGVVAQAGSFNMTDIVNSQAHVWNVIPQFFGFITFAIAGVAVCHRHPFDQPEAE
                     QELADGYHIEYSGMKFGLFFVGEYIGIVTISALMVTLFFGGWQGPLLPPFIWFALKTA
                     FFMMMFILIRASLPRPRYDQVMSFGWKICLPLTLINLLVTAAVILWQAQ"
     gene            complement(18211..20937)
                     /gene="nuoG"
                     /locus_tag="PN977_000132"
     CDS             complement(18211..20937)
                     /gene="nuoG"
                     /locus_tag="PN977_000132"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311194.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HDI6040219.1"
                     /translation="MATIHVDGKEYEVNGADNLLEACLSLGLDIPYFCWHPALGSVGA
                     CRQCAVKQYQNAEDTRGRLVMSCMTPASDGTFISIDDEEAKQFRESVVEWLMTNHPHD
                     CPVCEEGGNCHLQDMTVMTGHSFRRYRFTKRTHRNQDLGPFISHEMNRCIACYRCVRY
                     YKDYADGTDLGVYGAHDNVYFGRPEDGTLESEFSGNLVEICPTGVFTDKTHSERYNRK
                     WDMQFAPSICQQCSIGCNISPGERYGELRRIENRYNGTVNHYFLCDRGRFGYGYVNLK
                     DRPRQPVQRRGDDFITLNAEQAMQGAADILRQSKKVIGIGSPRASVESNFALRELVGE
                     ENFYTGIAHGEQERLQLALKVLREGGIYTPALREIESYDAVLVLGEDVTQTGARVALA
                     VRQAVKGKAREMAAAQKVADWQIAAILNIGQRAKHPLFVTNVDDTRLDDIAAWTYRAP
                     VEDQARLGFAIAHALDNSAPAVDGIEPELQSKIDVIVQALAGAKKPLIISGTNAGSAE
                     VIQAAANVAKALKGRGADVGITMIARSVNSMGLGIMGGGSLEEALTELETGRADAVVV
                     LENDLHRHASATRVNAALAKAPLVMVVDHQRTAIMENAHLVLSAASFAESDGTVINNE
                     GRAQRFFQVYDPAYYDSKTVMLESWRWLHSLHSTLLSREVDWTQLDHVIDAVVAKIPE
                     LAGIKDAAPDATFRIRGQKLAREPHRYSGRTAMRANISVHEPRQPQDIDTMFTFSMEG
                     NNQPTAHRSQVPFAWAPGWNSPQAWNKFQDEVGGKLRFGDPGVRLFETSENGLDYFTS
                     VPARFQPQDGKWRIAPYYHLFGSDELSQRAPVFQSRMPQPYIKLNPADAAKLGVNAGT
                     RVSFSYDGNTVTLPVEIAEGLTAGQVGLPMGMSGIAPVLAGAHLEDLKEAQQ"
     gene            complement(20990..22327)
                     /gene="nuoF"
                     /locus_tag="PN977_000133"
     CDS             complement(20990..22327)
                     /gene="nuoF"
                     /locus_tag="PN977_000133"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HDI6040220.1"
                     /translation="MKNIIRTPETHPLTWRLRDDKQPVWLDEYRSKNGYEGARKALTG
                     LSPDEIVNQVKDAGLKGRGGAGFSTGLKWSLMPKDESMNIRYLLCNADEMEPGTYKDR
                     LLMEQLPHLLVEGMLISAFALKAYRGYIFLRGEYIEAAVNLRRAIAEATEAGLLGKNI
                     MGTGFDFELFVHTGAGRYICGEETALINSLEGRRANPRSKPPFPATSGAWGKPTCVNN
                     VETLCNVPAILANGVEWYQNISKSKDAGTKLMGFSGRVKNPGLWELPFGTTAREILED
                     YAGGMRDGLKFKAWQPGGAGTDFLTEAHLDLPMEFESIGKAGSRLGTALAMAVDHEIN
                     MVSLVRNLEEFFARESCGWCTPCRDGLPWSVKILRALERGEGQPGDIETLEQLCRFLG
                     PGKTFCAHAPGAVEPLQSAIKYFREEFEAGIKQPFSNTHLINGIQPNLLKERW"
     gene            complement(22324..22824)
                     /gene="nuoE"
                     /locus_tag="PN977_000134"
     CDS             complement(22324..22824)
                     /gene="nuoE"
                     /locus_tag="PN977_000134"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HDI6040221.1"
                     /translation="MHENQQPQTEAFELSAAEREAIEHEMHHYEDPRAASIEALKIVQ
                     KQRGWVPDGAIHAIADVLGIPASDVEGVATFYSQIFRQPVGRHVIRYCDSVVCHINGY
                     QGIQAALEKKLNIKPGQTTFDGRFTLLPTCCLGNCDKGPNMMIDEDTHAHLTPEAIPE
                     LLERYK"
     gene            complement(22827..24629)
                     /gene="nuoC"
                     /locus_tag="PN977_000135"
     CDS             complement(22827..24629)
                     /gene="nuoC"
                     /locus_tag="PN977_000135"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C/D"
                     /protein_id="HDI6040222.1"
                     /translation="MVNNMTDLTAQEPAWQTRDHLDDPVIGELRNRFGPDAFTVQATR
                     TGVPVVWIKREQLLEVGDFLKKLPKPYVMLFDLHGMDERLRTHREGLPAADFSVFYHL
                     ISIDRNRDIMLKVALAENDLHVPTFTKLFPNANWYERETWDLFGITFDGHPNLRRIMM
                     PQTWKGHPLRKDYPARATEFSPFELTKAKQDLEMEALTFKPEEWGMKRGTENEDFMFL
                     NLGPNHPSAHGAFRIVLQLDGEEIVDCVPDIGYHHRGAEKMGERQSWHSYIPYTDRIE
                     YLGGCVNEMPYVLAVEKLAGITVPDRVNVIRVMLSELFRINSHLLYISTFIQDVGAMT
                     PVFFAFTDRQKIYDLVEAITGFRMHPAWFRIGGVAHDLPRGWDRLLREFLDWMPKRLA
                     SYEKAALQNTILKGRSQGVAAYGAKEALEWGTTGAGLRATGIDFDVRKARPYSGYENF
                     DFEIPVGGGVSDCYTRVMLKVEELRQSLRILEQCLNNMPEGPFKADHPLTTPPPKERT
                     LQHIETLITHFLQVSWGPVMPANESFQMIEATKGINSYYLTSDGSTMSYRTRIRTPSY
                     AHLQQIPAAIRGSLVSDLIVYLGSIDFVMSDVDR"
     gene            complement(24723..25385)
                     /gene="nuoB"
                     /locus_tag="PN977_000136"
     CDS             complement(24723..25385)
                     /gene="nuoB"
                     /locus_tag="PN977_000136"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoB"
                     /protein_id="HDI6040223.1"
                     /translation="MDYTLTRIDPNGENDRYPLQKQEIVTDPLEQEVNKNVFMGKLND
                     MVNWGRKNSIWPYNFGLSCCYVEMVTSFTAVHDVARFGAEVLRASPRQADLMVVAGTC
                     FTKMAPVIQRLYDQMLEPKWVISMGACANSGGMYDIYSVVQGVDKFIPVDVYIPGCPP
                     RPEAYMQALMLLQESIGKERRPLSWVVGDQGVYRANMQSERERKRGERIAVTNLRTPD
                     EI"
     gene            complement(25401..25844)
                     /gene="nuoA"
                     /locus_tag="PN977_000137"
     CDS             complement(25401..25844)
                     /gene="nuoA"
                     /locus_tag="PN977_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoA"
                     /protein_id="HDI6040224.1"
                     /translation="MSMSTSTEVIAHHWAFAIFLIVAIGLCCLMLVGGWFLGGRARAR
                     SKNVPFESGIDSVGSARLRLSAKFYLVAMFFVIFDVEALYLFAWSTSIRESGWVGFVE
                     AAIFIFVLLAGLVYLVRIGALDWTPARSRRERMNPETNSIANRQR"
     gene            complement(26474..27412)
                     /gene="lrhA"
                     /locus_tag="PN977_000138"
     CDS             complement(26474..27412)
                     /gene="lrhA"
                     /locus_tag="PN977_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LrhA"
                     /protein_id="HDI6040225.1"
                     /translation="MISANRPIINLDLDLLRTFVAVADLNTFAAAAAAVCRTQSAVSQ
                     QMQRLEQLVGKELFARHGRNKLLTEHGIQLLGYARKILRFNDEACSSLMFSNLQGVLT
                     IGASDESADTILPFLLNRVSSVYPKLALDVRVKRNAYMAEMLESQEVDLMVTTHRPST
                     FKALNLRTSPTHWYCAAEYVLQKGEPIPLVLLDDPSPFRDMVLATLNKADIPWRLAYV
                     ASTLPAVRAAVKAGLGVTARPVEMMSPDLRVLSGVDGLPPLPDTEYLLCYDPSSNNEL
                     AQVIYQAMESYHNPWQYSPMSAPEGDDSLLIERDIE"
     gene            28332..29549
                     /gene="alaA"
                     /locus_tag="PN977_000139"
     CDS             28332..29549
                     /gene="alaA"
                     /locus_tag="PN977_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase AlaA"
                     /protein_id="HDI6040226.1"
                     /translation="MSPIEKSSKLENVCYDIRGPVLKEAKRLEEEGNKVLKLNIGNPA
                     PFGFDAPDEILVDVIRNLPTAQGYCDSKGLYSARKAIMQHYQARGMRDVTVEDIYIGN
                     GVSELIVQAMQALLNSGDEMLVPAPDYPLWTAAVSLSSGKAVHYLCDESSDWFPDLDD
                     IRAKITPRTRGIVIINPNNPTGAVYSKELLMEIVEIARQHNLIIFADEIYDKILYDDA
                     EHHSIAPLAPDLLTITFNGLSKTYRVAGFRQGWMVLNGPKKHAKGYIEGLEMLASMRL
                     CANVPAQHAIQTALGGYQSISEFITPGGRLYEQRNRAWELINDIPGVSCVKPRGALYM
                     FPKIDAKRFNIHDDQKMVLDFLLQEKVLLVQGTAFNWPWPDHFRIVTLPRVDDIELSL
                     SKFARFLSGYHQL"
     gene            29633..30232
                     /gene="yfbR"
                     /locus_tag="PN977_000140"
     CDS             29633..30232
                     /gene="yfbR"
                     /locus_tag="PN977_000140"
                     /EC_number="3.1.3.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-deoxynucleotidase"
                     /protein_id="HDI6040227.1"
                     /translation="MKQSHFFAHLSRLKLINRWPLMRNVRTENVSEHSLQVAMVAHAL
                     AAIKNRKFGGNVNAERIALLAMYHDASEVLTGDLPTPVKYFNSQIAQEYKAIEKIAQQ
                     KLVDMVPEELRDIFAPLIDEHAYSDEEKSLVKQADALCAYLKCLEELAAGNNEFLLAK
                     TRLEATLEARRSQEMDYFMEVFVPSFHLSLDEISQDSPL"
     gene            complement(30291..32123)
                     /locus_tag="PN977_000141"
     CDS             complement(30291..32123)
                     /locus_tag="PN977_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HDI6040228.1"
                     /translation="MNGELIWVLSLLAVAIVLFATGRVRMDAVALFVIVAFALSGTLT
                     VPEVFSGFSDPNVVLIAALFIIGDGLVRTGVATVMGTWLVKVAGNSEIKMLVLLMLTV
                     AGLGAFMSSTGVVAIFIPVVLSVAMRMQTSPSRLMMPLSFAGLISGMMTLVATPPNLV
                     VNSELLREGYHGFSFFSVTPIGLVVLVLGILYMLVMRFMLKGDTQTPQREGWTRRTFR
                     DLIREYRLTGRARRLAIRPGSPMIGQRLDDLKLRERYGANVIGVERWRRFRRVIVNVN
                     GVSEFRARDVLLIDMSAADVDLRQFCSEQLLEPMVLRGEYFSDQALDVGMAEISLIPE
                     SELIGKSVREIGFRTRYGLNVVGLKRNGEAMEGSLADEPLLLGDIILVVGNWKLIGML
                     AKQGRDFVALNLPEEVSEASPAHSQAPHAIFCLVLMVALMLTDEIPNPVAAIIACLLM
                     GKFRCIDAESAYKSIHWPSIILIVGMMPFAVALQKTGGVALAVKGLMDIGGGYGPHMM
                     LGCLFVLSAVIGLFISNTATAVLMAPIALAAAKTMGVSPYPFAMVVAMAASAAFMTPV
                     SSPVNTLVLGPGNYSFSDFVKLGVPFTIIVMAVCVVMIPMLFPF"
     gene            complement(32210..32860)
                     /gene="hxpA"
                     /locus_tag="PN977_000142"
     CDS             complement(32210..32860)
                     /gene="hxpA"
                     /locus_tag="PN977_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpA"
                     /protein_id="HDI6040229.1"
                     /translation="MRCKGFLFDLDGTLVDSLPAVERAWSNWARRHGLAPEEVLAFIH
                     GKQAITSLRHFMAGKSEADIAAEFTRLEQIEATETEGITALPGAIALLNHLNKAGIPW
                     AIVTSGSMPVARARHKVAGLPAPEVFVTAERVKRGKPEPDAYLLGAQLLGLAPQECVV
                     VEDAPAGVLSGLAAGCHVIAVNAPADTPRLNEVDLVLHSLEQITVTKQPNGDVIIQ"
     gene            complement(32871..33365)
                     /locus_tag="PN977_000143"
     CDS             complement(32871..33365)
                     /locus_tag="PN977_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007371080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HDI6040230.1"
                     /translation="MEMTNAQRLILSNQYKMMTMLDPANAERYRRLQTIIERGYGLQM
                     RELDREFGELKEETCRTIIDIMEMYHALHVSWSNLQDQQSIDERRVTFLGFDAATEAR
                     YLGYVRFMVNVEGRYTHFDAGTHGFNAQTPMWEKYQRMLNVWHACPRQYHLSANEINQ
                     IINA"
     gene            complement(33448..33903)
                     /locus_tag="PN977_000144"
     CDS             complement(33448..33903)
                     /locus_tag="PN977_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF412 domain-containing protein"
                     /protein_id="HDI6040231.1"
                     /translation="MSTPDNRSVNFFSLFRRGQHYSKTWPLEKRLAPVFVENRVIKMT
                     RYAIRFMPPIAVFTLCWQIALGGQLGPAVATALFALSLPMQGLWWLGKRSVTPLPPAI
                     LNWFYEVRGKLQESGQVLAPVEGKPDYQALADTLKRAFKQLDKTFLDDL"
     gene            34241..35443
                     /gene="ackA"
                     /locus_tag="PN977_000145"
     CDS             34241..35443
                     /gene="ackA"
                     /locus_tag="PN977_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HDI6040232.1"
                     /translation="MSSKLVLVLNCGSSSLKFAIIDAVNGEEYLSGLAECFHLPEARI
                     KWKMDGNKQEAALGAGAAHSEALNFIVNTILAQKPELSAQLTAIGHRIVHGGEKYTSS
                     VVIDESVIQGIKDAASFAPLHNPAHLIGIEEALKSFPQLKDKNVAVFDTAFHQTMPEE
                     SYLYALPYNLYKEHGIRRYGAHGTSHFYVTQEAAKMLNKPVEELNIITCHLGNGGSVS
                     AIRNGKCVDTSMGLTPLEGLVMGTRSGDIDPAIIFHLHDTLGMSVDAINKLLTKESGL
                     LGLTEVTSDCRYVEDNYATKEDAKRAMDVYCHRLAKYIGAYTALMDGRLDAVVFTGGI
                     GENAAMVRELSLGKLGVLGFEVDHERNLAARFGKSGFINKEGTRPAVVIPTNEELVIA
                     QDASRLTA"
     gene            35518..37662
                     /gene="pta"
                     /locus_tag="PN977_000146"
     CDS             35518..37662
                     /gene="pta"
                     /locus_tag="PN977_000146"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HDI6040233.1"
                     /translation="MSRIIMLIPTGTSVGLTSVSLGVIRAMERKGVRLSVFKPIAQPR
                     TGGDAPDQTTTIVRANSSTTTAAEPLKMSYVEGLLSSNQKDVLMEEIVANYHANTKDA
                     EVVLVEGLVPTRKHQFAQSLNYEIAKTLNAEIVFVMSQGTDTPEQLKERIELTRNSFG
                     GAKNTNITGVIVNKLNAPVDEQGRTRPDLSEIFDDSTKAKVNNVDPAKLQESSPLPVL
                     GAVPWSFDLIATRAIDMARHLNATIINEGDINTRRVKSVTFCARSIPHMLEHFRAGSL
                     LVTSADRPDVLVAACLAAMNGVEIGALLLTGGYEMDARISKLCERAFATGLPVFMVNT
                     NTWQTSLSLQSFNLEVPVDDHERIEKVQEYVANYINADWIDSLTATSERSRRLSPPAF
                     RYQLTELARKAGKRIVLPEGDEPRTVKAAAICAERGIATCVLLGNPAEINRVAASQGV
                     ELGAGIEIVDPEVVRESYVGRLVELRKNKGMTETVAREQLEDNVVLGTLMLEQDEVDG
                     LVSGAVHTTANTIRPPLQLIKTAPGSSLVSSVFFMLLPEQVYVYGDCAINPDPTAEQL
                     AEIAIQSADSAAAFGIEPRVAMLSYSTGTSGAGSDVEKVREATRLAQEKRPDLMIDGP
                     LQYDAAVMADVAKSKAPNSPVAGRATVFIFPDLNTGNTTYKAVQRSADLISIGPMLQG
                     MRKPVNDLSRGALVDDIVYTIALTAIQSAQQQ"
     gene            37852..39372
                     /gene="yfcC"
                     /locus_tag="PN977_000147"
     CDS             37852..39372
                     /gene="yfcC"
                     /locus_tag="PN977_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HDI6040234.1"
                     /translation="MSAITESKPTRRWAMPDTLVIIFFVAILTSLATWVVPVGMFDSQ
                     EVQYQVDGQTKTRKVVDPHSFRILTNEAGEPEYHRVQLFTTGDERPGLMNFPFEGLTS
                     GSKYGTAVGIIMFMLVIGGAFGIVMRTGTIDNGILALIRHTRGNEILFIPALFILFSL
                     GGAVFGMGEEAVAFAIIIAPLMVRLGYDSITTVLVTYIATQIGFASSWMNPFCVVVAQ
                     GIAGVPVLSGSGLRIVVWVIATLIGLIFTMVYASRVKKNPLLSRVHESDRFFREKQAD
                     VEQRPFTFGDWLVLIVLTAVMVWVIWGVIVNAWFIPEIASQFFTMGLVIGIIGVVFRL
                     NGMTVNTMASSFTEGARMMIAPALLVGFAKGILLLVGNGEAGDASVLNTILNSIANAI
                     SGLDNAVAAWFMLLFQAVFNFFVTSGSGQAALTMPLLAPLGDLVGVNRQVTVLAFQFG
                     DGFSHIIYPTSASLMATLGVCRVDFRNWLKVGATLLGLLFIMSSVVVIGAQLMGYH"
     gene            complement(39405..39947)
                     /gene="yfcD"
                     /locus_tag="PN977_000148"
     CDS             complement(39405..39947)
                     /gene="yfcD"
                     /locus_tag="PN977_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase YfcD"
                     /protein_id="HDI6040235.1"
                     /translation="MEQRRLASTEWVDIVNEENEVIAQASREQMRAQCLRHRATYIVV
                     HDGMGKILVQRRTETKDFLPGMLDATAGGVVQADEQLLESARREAEEELGIAGVPFAE
                     HGQFYFEDKNCRVWGALFSCVSHGPFALQEDEVSEVCWLTPEEITARCDEFTPDSLKA
                     LALWMKRNAKNEAVETETAE"
     gene            complement(40005..40556)
                     /gene="yfcE"
                     /locus_tag="PN977_000149"
     CDS             complement(40005..40556)
                     /gene="yfcE"
                     /locus_tag="PN977_000149"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311211.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase"
                     /protein_id="HDI6040236.1"
                     /translation="MKLMFASDIHGSLPATERVLELFAQSGAQWLVILGDVLNHGPRN
                     ALPEGYAPAKVAERLNEVAHKVIAVRGNCDSEVDQMLLHFPITAPWQQVLLEKQRLFL
                     THGHLFGPENLPALNQNDVLVYGHTHLPVAEQRGEIFHFNPGSVSIPKGGNPASYGML
                     DNDVLSVIALNDQSIIAQVAINP"
     gene            complement(40612..41256)
                     /gene="yfcF"
                     /locus_tag="PN977_000150"
     CDS             complement(40612..41256)
                     /gene="yfcF"
                     /locus_tag="PN977_000150"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase"
                     /protein_id="HDI6040237.1"
                     /translation="MSKPAITLWSDAHCFSPYVLSAWVALQEKGLSFHIKTIDLDSGE
                     HLQPTWQGYGQTRRVPLLQIDDFELSESSAIAEYLEDRFAPPTWERIYPLDLENRARA
                     RQIQAWLRSDLMPIREERPTDVVFAGAKKAPLTAEGKASAEKLFAMAKHLLALGRPNL
                     FGEWCIADTDLALMINRLVLHGDEVPERLVDYATFQWQRASVQRFIALSAKQSG"
     gene            41393..42040
                     /gene="yfcG"
                     /locus_tag="PN977_000151"
     CDS             41393..42040
                     /gene="yfcG"
                     /locus_tag="PN977_000151"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GSH-dependent disulfide bond oxidoreductase"
                     /protein_id="HDI6040238.1"
                     /translation="MIDLYFAPTPNGHKITLFLEEAGLDYRLIKVDLGKGGQFRPEFL
                     RISPNNKIPAIVDHSPADGGEPLSLFESGAILLYLAEKTGLFLSHETRERAATLQWLF
                     WQVGGLGPMLGQNHHFNHAAPQTIPYAIERYQVETQRLYHVLNKRLENSPWLGGENYS
                     IADIACWPWVNAWTRQRIDLAMYPAVKNWHERIRSRPATGQALLKAQLGDERSDS"
     gene            42097..42459
                     /gene="folX"
                     /locus_tag="PN977_000152"
     CDS             42097..42459
                     /gene="folX"
                     /locus_tag="PN977_000152"
                     /EC_number="5.1.99.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate 2'-epimerase"
                     /protein_id="HDI6040239.1"
                     /translation="MAQPAAIIRIKNLRLRTFIGIKEEEINNRQDIVINVTIHYPADK
                     ARTSEDINDALNYRTVTKNIIQHVENNRFSLLEKLTQDVLDIAREHHWVTYAEVEIDK
                     LHALRYADSVSMTLSWQR"
     gene            42480..43373
                     /locus_tag="PN977_000153"
     CDS             42480..43373
                     /locus_tag="PN977_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HDI6040240.1"
                     /translation="MKIVITGGTGLIGRHLIPRLLDLGHQITVVTRNPQKASSVLGPR
                     VTLWQGLADQSNLNGFDAVINLAGEPIADKRWTHEQKERLCQSRWNITQKLVDLINAS
                     DTPPSVLISGSATGYYGDLGEVVVTEEEPPHNEFTHKLCARWEEIACRAQSDKTRVCL
                     LRTGVVLAPDGGILGKMLPPFRLGLGGPIGSGRQYLAWIHIDDMVNGILWLLDNELRG
                     PFNMVSPYPVRNEQFAHALGHALHRPAILRVPATAIRLLMGESSVLVLGGQRALPKRL
                     EEAGFAFRWYDLEEALADVVR"
     gene            complement(43421..44323)
                     /gene="rpnB"
                     /locus_tag="PN977_000154"
     CDS             complement(43421..44323)
                     /gene="rpnB"
                     /locus_tag="PN977_000154"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnB"
                     /protein_id="HDI6040241.1"
                     /translation="MTISTTSTPHDAVFKSFLRHPDTARDFIDIHLPAPLRKLCDLTT
                     LKLEPNSFIDEDLRQYYSDLLWSVKTQEGAGYIYVVIEHQSKPEELMAFRMMRYSIAA
                     MQNHLDAGYKELPLVIPMLFYHGCRSPYPYSLCWLDEFAEPAIARKIYSSAFPLVDIT
                     VVPDDEIMQHRKMALLELIQKHIRQRDLLGLVDQIVSLLVTGNTNDRQLKALFNYVLQ
                     TGDAQRFRAFIGEIAERAPQEKEKLMTIADRLREEGRNDGLILGKREEALRIAQEMLE
                     RGLDRELVMMVTRLSPDDLIAQSH"
     gene            complement(44520..45293)
                     /gene="hisP"
                     /locus_tag="PN977_000155"
     CDS             complement(44520..45293)
                     /gene="hisP"
                     /locus_tag="PN977_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter ATP-binding protein
                     HisP"
                     /protein_id="HDI6040242.1"
                     /translation="MSENKLNVIDLHKRYGEHEVLKGVSLQANAGDVISIIGSSGSGK
                     STFLRCINFLEKPSEGSIVVNGQTINLVRDKDGQLKVADKNQLRLLRTRLTMVFQHFN
                     LWSHMTVLENVMEAPIQVLGLSKQEARERAVKYLAKVGIDERAQGKYPVHLSGGQQQR
                     VSIARALAMEPEVLLFDEPTSALDPELVGEVLRIMQQLAEEGKTMVVVTHEMGFARHV
                     STHVIFLHQGKIEEEGAPEQLFGNPQSPRLQQFLKGSLK"
     gene            complement(45301..46017)
                     /locus_tag="PN977_000156"
     CDS             complement(45301..46017)
                     /locus_tag="PN977_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDI6040243.1"
                     /translation="MIEILHEYWKPLLWTDGYRFTGVAITLWLLILSVVIGGVLALFL
                     AIGRVSSNKYIQFPIWLFTYIFRGTPLYVQLLVFYSGMYTLEIVKGTEFLNAFFRSGL
                     NCTVLALTLNTCAYTTEIFAGAIRSVPHGEIEAARAYGFSTFKMYRCIILPSALRIAL
                     PAYSNEVILMLHSTALAFTATVPDLLKIARDINAATYQPFTAFGIAAVLYLIISYVLI
                     SLFRRAEKRWLQHVKPSSTH"
     gene            complement(46014..46700)
                     /gene="hisQ"
                     /locus_tag="PN977_000157"
     CDS             complement(46014..46700)
                     /gene="hisQ"
                     /locus_tag="PN977_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012124147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter permease HisQ"
                     /protein_id="HDI6040244.1"
                     /translation="MLYGFSGVILQGALVTLELAISSVVLAVIIGLIGAGGKLSQNRL
                     SGLIFEGYTTLIRGVPDLVLMLLIFYGLQIALNTVTEAMGVGQIDIDPMVAGIITLGF
                     IYGAYFTETFRGAFMAVPKGHIEAATAFGFTRGQVFRRIMFPAMMRYALPGIGNNWQV
                     ILKSTALVSLLGLEDVVKATQLAGKSTWEPFYFAIVCGVIYLVFTTVSNGVLLFLERR
                     YSVGVKRADL"
     gene            complement(46790..47572)
                     /gene="hisJ"
                     /locus_tag="PN977_000158"
     CDS             complement(46790..47572)
                     /gene="hisJ"
                     /locus_tag="PN977_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter substrate-binding
                     protein HisJ"
                     /protein_id="HDI6040245.1"
                     /translation="MKKLVLSLSLVLAFSSATAAFAAIPQNIRIGTDPTYAPFESKNS
                     QGELVGFDIDLAKELCKRINTQCTFVENPLDALIPSLKAKKIDAIMSSLSITEKRQQE
                     IAFTDKLYAADSRLVVAKNSDIQPTVESLKGKRVGVLQGTTQETFGNEHWAPKGIEIV
                     SYQGQDNIYSDLTAGRIDAAFQDEVAASEGFLKQPVGKDYKFGGPSVKDEKLFGVGTG
                     MGLRKEDNELREALNKAFAEMRADGTYEKLAKKYFDFDVYGG"
     gene            complement(47793..48575)
                     /gene="argT"
                     /locus_tag="PN977_000159"
     CDS             complement(47793..48575)
                     /gene="argT"
                     /locus_tag="PN977_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine/arginine/ornithine ABC transporter
                     substrate-binding protein ArgT"
                     /protein_id="HDI6040246.1"
                     /translation="MKKSILALSLLVGLSAAASSYAALPETVRIGTDTTYAPFSSKDA
                     KGDFVGFDIDLGNEMCKRMQVKCTWVASDFDALIPSLKAKKIDAIISSLSITDKRQQE
                     IAFSDKLYAADSRLIAAKGSPIQPTLDSLKGKHVGVLQGSTQEAYANDNWRSKGVDVV
                     AYANQDLVYSDLAAGRLDAALQDEVAASEGFLKQPAGKDFAFAGPSVKDKKYFGDGTG
                     VGLRKDDAELTAAFNKALNELRQDGTYDKMAKKYFDFNVYGD"
     gene            complement(48841..49410)
                     /gene="ubiX"
                     /locus_tag="PN977_000160"
     CDS             complement(48841..49410)
                     /gene="ubiX"
                     /locus_tag="PN977_000160"
                     /EC_number="2.5.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin prenyltransferase UbiX"
                     /protein_id="HDI6040247.1"
                     /translation="MKRLIVGISGASGAIYGVRLLQVLRDVTDIETHLVMSQAARQTL
                     SLETDFSLREVQALADVTHDARDIAASISSGSFQTLGMVILPCSIKTLSGIVHSYTDG
                     LLTRAADVVLKERRPLVLCVRETPLHLGHLRLMTQAAEIGAVIMPPVPAFYHRPQSLD
                     DVINQTVNRVLDQFAITLPEDLFARWQGA"
     gene            complement(49505..51022)
                     /gene="purF"
                     /locus_tag="PN977_000161"
     CDS             complement(49505..51022)
                     /gene="purF"
                     /locus_tag="PN977_000161"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HDI6040248.1"
                     /translation="MCGIVGIAGVMPVNQSIYDALTVLQHRGQDAAGIITIDANNCFR
                     LRKANGLVSDVFEARHMQRLQGNMGIGHVRYPTAGSSSASEAQPFYVNSPYGITLAHN
                     GNLTNAHELRKKLFEEKRRHINTTSDSEILLNIFASELDNFRHYPLEADNIFAAIAAT
                     NRLIRGAYACVAMIIGHGMVAFRDPNGIRPLVLGKRDIDENRTEYMVASESVALDTLG
                     FDFLRDVAPGEAIYITEEGQLFTRQCADNPVSNPCLFEYVYFARPDSFIDKISVYSAR
                     VNMGTKLGEKIAREWEDLEIDVVIPIPETSCDIALEIARILGKPYRQGFVKNRYVGRT
                     FIMPGQQLRRKSVRRKLNANRAEFRDKNVLLVDDSIVRGTTSEQIIEMAREAGAKKVY
                     LASAAPEIRFPNVYGIDMPSATELIAHGREVDEIRQIIGADGLIFQDLNDLIEAVRAE
                     NPDIQQFECSVFNGVYVTKDVDQGYLDFLDTLRNDDAKAVQRQNEVENLEMHNEG"
     gene            complement(51059..51547)
                     /gene="cvpA"
                     /locus_tag="PN977_000162"
     CDS             complement(51059..51547)
                     /gene="cvpA"
                     /locus_tag="PN977_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin V production protein"
                     /protein_id="HDI6040249.1"
                     /translation="MVWIDYAIIAVIAFSSLVSLIRGFVREALSLVTWGCAFFVASHY
                     YTYLSVWFTGFEDELVRNGIAIAVLFIATLIVGAIVNFVIGQLVEKTGLSGTDRVLGV
                     CFGALRGVLIVAAILFFLDSFTGVSKSEDWSKSQLIPQFSFIIRWFFDYLQSSSSFLP
                     RA"
     gene            complement(51783..52445)
                     /gene="dedD"
                     /locus_tag="PN977_000163"
     CDS             complement(51783..52445)
                     /gene="dedD"
                     /locus_tag="PN977_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DedD"
                     /protein_id="HDI6040250.1"
                     /translation="MASKFQNRLVGTIVLVALGVIVLPGLLDGQKKHYQDEFAAIPLV
                     PKAGDRDEPDMMPAATQALPTQPPEGAAEEVRAGDAAAPSLDPATIAANNTEFEPEPA
                     PVVPPKPKPVEPPKPKVEAPPAPKPEPKPVVEEKAAPTGKAYVVQLGALKNADKVNEI
                     VGKLRGAGYRVYTSPSTPVQGKITRILVGPDASKDKLKGSLGELKQLSGLSGVVMGYT
                     PN"
     gene            complement(52435..53703)
                     /gene="folC"
                     /locus_tag="PN977_000164"
     CDS             complement(52435..53703)
                     /gene="folC"
                     /locus_tag="PN977_000164"
                     /EC_number="6.3.2.12"
                     /EC_number="6.3.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tetrahydrofolate
                     synthase/dihydrofolate synthase"
                     /protein_id="HDI6040251.1"
                     /translation="MIIKRTPQAASPLASWLSYLENLHSKTIDLGLERVSQVAARLGV
                     LKPAPFVFTVAGTNGKGTTCRTLESILMAAGYKVGVYSSPHLVRYTERVRVQGQELPE
                     SAHTASFAEIESARGDISLTYFEYGTLSALWLFKQAQLDVVILEVGLGGRLDATNIVD
                     ADVAVVTSIALDHTDWLGPDRESIGREKAGIFRSEKPAIVGEPEMPSTIADVAQEKGA
                     MLQRRGVEWNYSVTDHDWTFSDAHGTLENLPLPLVPQPNAATALAALRASGLEVSENA
                     IRDGIASAILPGRFQIVSESPRVIFDVAHNPHAAEYLTGRMKALPKNGRVLAVIGMLH
                     DKDIAGTLAWLKSVVDDWYCAPLEGPRGATAEQLLEHLGNGKSFDSVAQAWDAAMADA
                     KAEDTVLVCGSFHTVAHVMEVIDARRSGGK"
     gene            complement(53773..54687)
                     /gene="accD"
                     /locus_tag="PN977_000165"
     CDS             complement(53773..54687)
                     /gene="accD"
                     /locus_tag="PN977_000165"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HDI6040252.1"
                     /translation="MSWIERIKSNITPTRKASIPEGVWTKCDSCGQVLYRAELERNLE
                     VCPKCDHHMRMTARNRLHSLLDEGSLVELGSELEPKDVLKFRDSKKYKDRLASAQKET
                     GEKDALVVMKGTLYGMPVVAAAFEFAFMGGSMGSVVGARFVRAVEQALEDNCPLICFS
                     ASGGARMQEALMSLMQMAKTSAALAKMQERGLPYISVLTDPTMGGVSASFAMLGDLNI
                     AEPKALIGFAGPRVIEQTVREKLPPGFQRSEFLIEKGAIDMIVRRPEMRLKLASILAK
                     LMNLPAPNPEAPREGVVVPPVPDQEPEA"
     gene            complement(54843..55502)
                     /locus_tag="PN977_000166"
     CDS             complement(54843..55502)
                     /locus_tag="PN977_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HDI6040253.1"
                     /translation="MDLIYFLIDFILHIDVHLAELVAEYGVWVYAILFLILFCETGLV
                     VTPFLPGDSLLFVAGALASLETNDLNVHMMVVLMLIAAIVGDAVNYTIGRLFGDKLFS
                     NPDSKIFRRSYLDKTHQFYEKHGGKTIILARFVPIVRTFAPFVAGMGHMSYRHFAAFN
                     VIGALLWVLIFTYAGYFFGTLPFIQSNLKLMIVGIIFVSILPGVFEFIRHKRAAARAA
                     K"
     gene            complement(55585..56397)
                     /gene="truA"
                     /locus_tag="PN977_000167"
     CDS             complement(55585..56397)
                     /gene="truA"
                     /locus_tag="PN977_000167"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HDI6040254.1"
                     /translation="MSDQQQPPVYKIALGIEYDGSKYYGWQRQNEVRSVQEKLEKALS
                     QVANEPITVFCAGRTDAGVHGTGQVVHFETTAQRKDAAWTLGVNANLPGDIAVRWVKA
                     VPDDFHARFSATARRYRYIIYNHRLRPAVLSKGVTHFYEPLDAERMHRAAQCLLGEND
                     FTSFRAVQCQSRTPWRNVMHINVTRHGPYVVVDIKANAFVHHMVRNIVGSLMEVGAHN
                     QPESWIAELLAAKDRTLAAATAKAEGLYLVAVDYPDRYDLPKPPMGPLFLAD"
     gene            complement(56397..57410)
                     /locus_tag="PN977_000168"
     CDS             complement(56397..57410)
                     /locus_tag="PN977_000168"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HDI6040255.1"
                     /translation="MSEGWNIAVLGATGAVGEALLETLAERQFPVGEIYALARNESAG
                     EQLRFGGKTITVQDAAEFDWTQAQLAFFVAGKEATAAWVEEATNSGCLVIDSSGLFAL
                     EPDVPLVVPEVNPFVLTDYRNRNVIAVPDSLTSQLLAALKPLIDQGGLSRISVTSLIS
                     ASAQGKKAVDALAGQSAKLLNGIPIDEEDFFGRQLAFNMLPLLPDSEGSVREERRIVD
                     EVRKILQDEGLMISASVVQAPVFYGHAQMVNFEALRPLAAEEARDAFAQGEDIVLSEE
                     NEFPTQVGDASGTPHLSVGCVRNDYGMPEQVQFWSVADNVRFGGALMAVKIAEKLVQE
                     YLY"
     gene            complement(57476..58612)
                     /gene="pdxB"
                     /locus_tag="PN977_000169"
     CDS             complement(57476..58612)
                     /gene="pdxB"
                     /locus_tag="PN977_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-phosphoerythronate dehydrogenase PdxB"
                     /protein_id="HDI6040256.1"
                     /translation="MKILVDENMPYARDLFSRLGEVTAVPGRPIPVAQLADADALMVR
                     SVTKVNESLLAGKPIKFVGTATAGTDHVDEAWLKQAGIGFSAAPGCNAIAVVEYVFSS
                     LLMLAERDGFSLHERTVGIVGVGNVGRRLQARLEALGIKTLLCDPPRADRGDEGDFRS
                     LDELVQHADILTFHTPLFKDGPYKTLHLADEKLIRSLKPGAILINACRGAVVDNTALL
                     TCLNEGQKLSVVLDVWEGEPELNVELLKKVDIGTPHIAGYTLEGKARGTTQVFEAYSK
                     FIGHEQHVALDTLLPAPEFGRITLHGPLDQPTLKRLVHLVYDVRRDDAPLRKVAGIPG
                     EFDKLRKNYLERREWSSLYVICDDASAASLLCKLGFNAVHHPAR"
     gene            58711..59706
                     /gene="flk"
                     /locus_tag="PN977_000170"
     CDS             58711..59706
                     /gene="flk"
                     /locus_tag="PN977_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulator Flk"
                     /protein_id="HDI6040257.1"
                     /translation="MIQPISGPPPGQPPGQGDNLPSGAGNQPLSSQQRTSLESLMTKV
                     TSLTQQQRAELWAGIRHDIGLSGDSPLLSRHFPAAEHNLAQRLLAAQKSHSARQLLAQ
                     LGEYLRLGNNRQAVTEYIRHNFGQTPLNQLSPEQLKTILTLLQEGKMVIPQPQQREAT
                     DRPLLPAEHNALKQLVTKLAAATGEPSKQIWQSMLELSGVKDGELIPAKLFNHLVTWL
                     QARQTLSQQNTPTLESLQMALKQPLDASELAALSTYIQQKYGLSAQSSLSSFQAEDIL
                     NQLYQRRVKGIEPRDMQPLLNPFPPMMDTLQNMATRPALWILLVAIILMLVWLVR"
     gene            complement(59703..60881)
                     /locus_tag="PN977_000171"
     CDS             complement(59703..60881)
                     /locus_tag="PN977_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDI6040258.1"
                     /translation="MTAVSQTETRSSANFSLFRIAFAVFLTYMTVGLPLPVIPLFVHH
                     ELGYGNTMVGIAVGIQFLATVLTRGYAGRLADQYGAKRSALQGMLACGLAGGALLLAA
                     ILPVSAPFKFALLVVGRLILGFGESQLLTGALTWGLGIVGPKHSGKVMSWNGMAIYGA
                     LAVGAPLGLLIHSHYGFAALALTTMALPLLAWACNGTVRKVPALAGERPSLWSVVGLI
                     WKPGLGLALQGVGFAVIGTFVSLYFASKGWAMAGFTLTAFGGAFVVMRVMFGWMPDRF
                     GGVKVAIVSLLVETVGLLLLWQAPGAWVALAGAALTGAGCSLIFPALGVEVVKRVPSH
                     VRGTALGGYAAFQDIALGVSGPLAGMLATTFGYSSVFLAGAISAVLGIIVTILSFRRG
                     "
     gene            complement(61113..62333)
                     /gene="fabB"
                     /locus_tag="PN977_000172"
     CDS             complement(61113..62333)
                     /gene="fabB"
                     /locus_tag="PN977_000172"
                     /EC_number="2.3.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase I"
                     /protein_id="HDI6040259.1"
                     /translation="MKRAVITGLGIVSSIGNNQQEVLASLREGRSGITFSQELKDSGM
                     RSHVWGNVKLDTTGLIDRKVVRFMSDASIYAFLSMEQAIADAGLSPEAYQNNPRVGLI
                     AGSGGGSPRFQVFGADAMRGPRGLKAVGPYVVTKAMASGVSACLATPFKIHGVNYSIS
                     SACATSAHCIGNAVEQIQLGKQDIVFAGGGEELCWEMACEFDAMGALSTKYNDTPEKA
                     SRTYDAHRDGFVIAGGGGMVVVEELEHALARGAHIYAEIVGYGATSDGADMVAPSGEG
                     AVRCMKMAMHGVDTPIDYLNSHGTSTPVGDVKELAAIREVFGDKSPAISATKAMTGHS
                     LGAAGVQEAIYSLLMLEHGFIAPSINIEELDEQAAGLNIVTETTDRELTTVMSNSFGF
                     GGTNATLVMRKLKD"
     gene            62492..64498
                     /gene="mnmC"
                     /locus_tag="PN977_000173"
     CDS             62492..64498
                     /gene="mnmC"
                     /locus_tag="PN977_000173"
                     /EC_number="1.5.-.-"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (5-methylaminomethyl-2-thiouridine)(34)-methyltransferase
                     MnmD/FAD-dependent
                     5-carboxymethylaminomethyl-2-thiouridine(34)
                     oxidoreductase MnmC"
                     /protein_id="HDI6040260.1"
                     /translation="MKHYAIQPANLEFNAEGTPVSRDFDDVYFSNDNGLEETRYVFLG
                     GNQLEARFPEHPHPLFVVAESGFGTGLNFLTLWQAFDQFREAHPQAQLQRLHFISFEK
                     FPLARADLALAHHHWPELAPWAEQLQAQWPMPLPGCHRLLFDEGHVTLDLWFGDINEL
                     TSQLDDSLNQKVDAWFLDGFAPAKNPDMWTQNLFKAMARLARPGGTLATFTSAGFVRR
                     GLQEAGFSMQKRKGFGRKREMLCGVMEQTLPLPCSTPWFNRTGSNKQEAAIIGGGIAS
                     ALLSLALLRRGWQVTLYCADEAPALGASGNRQGALYPLLSKHDEALNRFFSNAFTFAR
                     RFYDQLPVKFDHDWCGVTQLGWDEKSQHKIAQMLSMDLPAELAVAVEANAVEQITGVA
                     TNCSGITYPEGGWLCPAELTRNVLELAQQQGLQIRYQHQLQDLSRKDDGWLLNFAGDQ
                     QATHSVVVLANGHQISRFSQTSSLPVYSVAGQVSHIPTTPELAKLKQVLCYDGYLTPQ
                     NPANQHHCIGASYHRGSEDTAYSEDDQQQNRQRLIDCFPQAQWAKAVDVSDKEARCGV
                     RCATRDHLPMVGNVPDYDATLVEYASLAEKKDEAVSAPVYDDLFMFAALGSRGLCSAP
                     LCAEILAAQMSEEPIPMDASTLAALNPNRLWVRKLLKGKAVKAG"
     gene            complement(64554..64832)
                     /locus_tag="PN977_000174"
     CDS             complement(64554..64832)
                     /locus_tag="PN977_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcL family protein"
                     /protein_id="HDI6040261.1"
                     /translation="MIAEFESRILALIDGMVDYASDDELFASGYLRGHLTLAIAELES
                     GDDHSAQAVHTTVSQSLEKAIGAGELSPRDQALVTDMWEHLFQQASQQ"
     gene            complement(64866..65414)
                     /gene="epmC"
                     /locus_tag="PN977_000175"
     CDS             complement(64866..65414)
                     /gene="epmC"
                     /locus_tag="PN977_000175"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P hydroxylase"
                     /protein_id="HDI6040262.1"
                     /translation="MNSTHHYEQLIEIFNSCFADEFNTRLIKGDDEPIYLPADAEAPY
                     NRIVFAHGFYASAIHEISHWCIAGKARRELVDFGYWYCPDGRDAQTQSQFEDVEVKPQ
                     ALDWLFCVAAGYPFNVSCDNLEGDFEPDRVVFQRRVHAQVMDYLANGIPERPARFIKA
                     LQNYYHTPELTAEQFPWPEALN"
     gene            complement(65414..66223)
                     /locus_tag="PN977_000176"
     CDS             complement(65414..66223)
                     /locus_tag="PN977_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HDI6040263.1"
                     /translation="METFNSLFMVSPLLLGVLFFVAMLAGFIDSIAGGGGLLTIPALM
                     AAGMSPANALATNKLQACGGSISATIYFIRRKVVSLSDQKLNIAMTFVGSMSGALLVQ
                     YVQADVLRQILPILVICIGLYFLLMPKLGEEDRQRRMYGLPFALVAGGCVGFYDGFFG
                     PAAGSFYALAFVTLCGFNLAKATAHAKLLNATSNIGGLLLFILGGKVIWATGFVMLVG
                     QFLGARMGSRLVLSKGQKLIRPMIVIVSAVMSAKLLYDSHGQEILHWLGMN"
     gene            complement(66223..67047)
                     /gene="mepA"
                     /locus_tag="PN977_000177"
     CDS             complement(66223..67047)
                     /gene="mepA"
                     /locus_tag="PN977_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-insensitive murein endopeptidase"
                     /protein_id="HDI6040264.1"
                     /translation="MNKTAIALLALLASSASLAATPWQKITQPVPGSAQSIGSFSNGC
                     IVGADTLPIQSEHYQVMRTDQRRYFGHPDLVMFIQRLSRQVSNLGMGTVLIGDMGMPA
                     GGRFNGGHASHQTGLDVDIFLQLPKTRWTSAQLLRPQALDLVSRDGKHVVPALWKPEI
                     FSLIKLAAQDKDVTRIFVNPAIKQQLCLDAGTDRDWLRKVRPWFQHRAHMHVRLRCPA
                     DSLECEDQPLPPPGDGCGAELQSWFEPPKPGTTKPEKKTPPPLPPSCQALLDEHVI"
     gene            complement(67051..68136)
                     /gene="aroC"
                     /locus_tag="PN977_000178"
     CDS             complement(67051..68136)
                     /gene="aroC"
                     /locus_tag="PN977_000178"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HDI6040265.1"
                     /translation="MAGNTIGQLFRVTTFGESHGLALGCIVDGVPPGIPLTEADLQHD
                     LDRRRPGTSRYTTQRREPDQVKILSGVFEGVTTGTSIGLLIENTDQRSQDYSAIKDVF
                     RPGHADYTYEQKYGLRDYRGGGRSSARETAMRVAAGAIAKKYLAEKFGIEIRGCLTQM
                     GDIPLEIKDWSQVEQNPFFCPDPDKIDALDELMRALKKEGDSIGAKVTVVASGVPAGL
                     GEPVFDRLDADIAHALMSINAVKGVEIGDGFDVVALRGSQNRDEITKDGFQSNHAGGI
                     LGGISSGQQIIAHMALKPTSSITVPGRTINRFGEEVEMITKGRHDPCVGIRAVPIAEA
                     MLAIVLMDHLLRQRAQNADVKTDIPRW"
     gene            complement(68171..69103)
                     /gene="prmB"
                     /locus_tag="PN977_000179"
     CDS             complement(68171..69103)
                     /gene="prmB"
                     /locus_tag="PN977_000179"
                     /EC_number="2.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3 N(5)-glutamine
                     methyltransferase"
                     /protein_id="HDI6040266.1"
                     /translation="MDKIFVDEAVNELQTIQDMLRWSVSRFSAANIWYGHGTDNPWDE
                     AVQLVLPSLYLPLDIPEDMRTARLTSSEKHRIVERVIRRVNERIPVAYLTNKAWFCGH
                     EFYVDERVLVPRSPIGELINNKFAGLISKQPQHILDMCTGSGCIAIACAYAFPDAEVD
                     AVDISPDALAVAEQNIEEHGLIHNVIPIRSDLFRDLPKVQYDLIVTNPPYVDAEDMSD
                     LPNEYRHEPELGLASGTDGLKLTRRILGNAADYLADDGVLICEVGNSMVHLMEQYPDV
                     PFTWLEFDNGGDGVFMLTKEQLLAAREHFAIYKD"
     gene            69269..69820
                     /gene="smrB"
                     /locus_tag="PN977_000180"
     CDS             69269..69820
                     /gene="smrB"
                     /locus_tag="PN977_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease SmrB"
                     /protein_id="HDI6040267.1"
                     /translation="MKKKTTLSEEDQALFRQLMAGTRKIKQDTIVHRPQRKKISEVPV
                     KRLIQEQADASHYFSDEFQPLLNTEGPVKYVRPDVSHFEAKKLRRGDYSPELFLDLHG
                     LTQLQAKQELGALIAACRREHVFCACVMHGHGKHILKQQTPLWLAQHPHVMAFHQAPK
                     EYGGDAALLVLIEVDEWLPPELP"
     gene            69906..71264
                     /locus_tag="PN977_000181"
     CDS             69906..71264
                     /locus_tag="PN977_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000981116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDI6040268.1"
                     /translation="MMNIRTLKLTNLGRFEELEVHLAPVEEFKSNVTVFIGNNGAGKT
                     SILKSLATSLSWFVARVRTEKGNGSPIPEDAILNGRSSATIELQVLNTHPATEAATPY
                     RWLLARTASGKKSTTASSLQEASQLAAFYRDQYTQNSGASFPLIAFYPVERVVLDVPL
                     KIKERHNFLQLDGYDNALNQGIDFRRFFEWFRNREDAENESGLPQDVLDKLSTRIDLD
                     NTVLNALTAIMASSRDRQLTAVRTAISRFMPGFSNLRVRRKPRLHMSIDKNGQTLNVL
                     QLSQGEKSLMALVGDIARRLAMMNPMLENPLNGEGIVLIDEVDMHLHPTWQRTIIQRL
                     TTTFPHCQFVLTTHSPLVISDYKDVLVYSLDNGELTQLPSLYGQDANTVLLNVMDTDI
                     RNATVAEKLNDLLDLIQKNDFINANALLNTLSLELPENHLELVKARMLLRKQEIKHAR
                     NN"
     gene            71248..71907
                     /locus_tag="PN977_000182"
     CDS             71248..71907
                     /locus_tag="PN977_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016243072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6040269.1"
                     /translation="MREITKGQPPASLQQWVRAKPRDKNANQWFQELYAQKKWDIVGD
                     LSQQCAQEQFYLCAYCCDRVTGTNRDTVNEHVEARDLAPARSLEFTNIVASCKTKGQC
                     DDSHKNQPLLLTPLMPECETEFIFKISGRIEGTTPRAIDAIKVLNLGDSERNNRALIE
                     KRKQLSDSLLWANGIDPSEGLEDDDLLQAVIDELLTPVDGYLAPFAPVVANILKNWMT
                     A"
     gene            complement(71949..72788)
                     /locus_tag="PN977_000183"
     CDS             complement(71949..72788)
                     /locus_tag="PN977_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001317961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2544 domain-containing protein"
                     /protein_id="HDI6040270.1"
                     /translation="MKKWMMFFASLILLVMSVGTPKSYARDKLTSATFDSVKIYYGMG
                     RAGFTTGALHVTVLTPGEVAYGVYNTTGYIGTDARVVSWTGSGPAPTLTLVDYSSTDN
                     SFCPGINTTLFRCAYETFKVTVQSDNYGCPWIASFYEVSDQHAYGIYTGPTDHATACP
                     TIPVTSFDISWNENYVSHSKALQLQSNGSTITTTLSTYLMQDGKLCDGSVFDNRGAYC
                     RVVSELLTFTSYGCDKSTVTVSPTRHPVTDKQLHDIVVKVNTSSRQPIDSTCRFQYVL
                     NEL"
     gene            complement(72790..73314)
                     /locus_tag="PN977_000184"
     CDS             complement(72790..73314)
                     /locus_tag="PN977_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HDI6040271.1"
                     /translation="MTKKHALVFISSLLLLCSSTAPAVDNLHFTGNLLGKSCTPVING
                     SLLAEVQFPTIAASDLMHLGQSDRVPLVFQLKDCKSSTLFSVRVTLAGTEDSELPGFL
                     AIDASSSASGVGIGIETAAGAAVPINDTTGVTFPLNQGSNTLNFNAWLQTKSGRDVTP
                     GDFSATATATFEYF"
     gene            complement(73311..73781)
                     /locus_tag="PN977_000185"
     CDS             complement(73311..73781)
                     /locus_tag="PN977_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HDI6040272.1"
                     /translation="MKRISLLMLWGFCSMALSNVTFHGYLVQPPNCTISDGETIELTF
                     QDVNIDDINGSNYEQIVPYRITCDTPVRDPLLEMTLSWSGTQSDFDDAAVSTDIAGLG
                     IRLKQAGQSFKLNTPLVVDETALPALTAVPVKKSGVDLPEANFEAWATLQVDYQ"
     gene            complement(73778..74269)
                     /locus_tag="PN977_000186"
     CDS             complement(73778..74269)
                     /locus_tag="PN977_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HDI6040273.1"
                     /translation="MKGSWAWLLVGCIGSCSVTAGSKSVDMILRVLVDAPPPCTVTGA
                     EVEFGNVVITRIGGVNYKRPIDYKLVCNNLAMDDLRLQMQAATVVINGETVISTGIPG
                     FGIRIQKASDSSILDLTSGAWLPFNFSSGAPALEAVPVKQSGTSLTAAEFSASATIVV
                     DYQ"
     gene            complement(74286..75038)
                     /locus_tag="PN977_000187"
     CDS             complement(74286..75038)
                     /locus_tag="PN977_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000120669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HDI6040274.1"
                     /translation="MSYKLSSSMVVSTALAALLSTVSLAAHASVTPDRTRLVFNESDK
                     SISVTLRNNNEKLPYLAQSWLEDEKGNKITSPLAVLPPVQRIDAMMNGQVKIQALPDI
                     HTLPSDRESLFYYNVREIPPKTGKANTLQIALQTRIKLFWRPKALEKIDMRKPWQFKV
                     TLTRSGQDYTVNNPTPYHVIISDASTQKKGPTAAGFKPLVMSPKTSQPLKAKMDSAPV
                     LTYINDYGARLPLIFRCEGDTCKVDEDQSSKG"
     gene            complement(75058..77706)
                     /locus_tag="PN977_000188"
     CDS             complement(75058..77706)
                     /locus_tag="PN977_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HDI6040275.1"
                     /translation="MPNHSNFRLRGIACYIALAISGGSVNAWADDSIQFDPRFLELKG
                     DTKIDLGKFSKKGYVDAGKYNLRVFINKQPLSDEYDINWYVSENDPTKTYACLTPELV
                     AALGLKEGIAKSLQWTHNDECLKPGQLDGMEVENDLSQSALLLTVPQAYLEYTSSDWD
                     PPSRWDDGIPGLIADYSLNAQTRHQEQGGEDSHDISGNGTVGANLGAWRFRADWQSDY
                     QHTRSNDDDDDSSNSTTSKNWDWSRYYAWRALPSLKAKLSLGEDYLNSDIFDGFNYIG
                     SSVSTDDQMLPPNLRGYAPDVSGVAHSSAKVTISQMGRVLYETQVPAGPFRIQDIGDS
                     VSGTLHVRVEEQNGQVQEYDVTTASMPFLTRQGQVRYKVMMGRPEDWNHKTEGGFFSG
                     GEASWGVADGWSLYGGALADEHYQSAAMGVGRDLAQFGALAFDVTHSHVNLDHDSAYG
                     KGKLDGNSFRVSYAKDFDELNSRVTFAGYRFSEKNFMTMSEYLDANQSDMARTGNDKE
                     MYTITYNQNFAAAGVSIYLNYSHRTYWDRPEQTNYNLMFSHYFNMGSIRNVSISVTGY
                     RYEYDDNADKGMYLSMSIPWSDSSTVTYNGSYGSGSDSSQVGYFKRVDDATHYQVNVG
                     TSEQHGSVDGYLSHDGSLAKVDLSANYHEGEYRSAGIALQGGATLTAHGGALHRTQSM
                     GGTRLLIDADGIANVPVESNGAPVYTNMFGKAVVADINNYYRNQAYIDLNNLPEDAEA
                     TQSVVQATLTEGAIGYRKFKVISGQKAMAVLRLRDGSYPPFGAEVKNDEQQQVGIVDD
                     EGNVYLAGVNAGEHMTVFWEGSAQCEIVLPKPLPADLFSGLLLPCEQKGTAAPDSSAP
                     EIKPVIQDQTRQVTPTEAPTSISATQ"
     gene            complement(77787..78353)
                     /locus_tag="PN977_000189"
     CDS             complement(77787..78353)
                     /locus_tag="PN977_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000032634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HDI6040276.1"
                     /translation="MSKFAKTAIAAAMVMGAVVSSSAFAAGNNGTARFYGTIEDSPCS
                     IVPDDHKLEVDMGDIGAAKLTGGGTTTPKDFQIRLQDCVFSTETNMETTFTGTNYAGP
                     ANTDNYALFNVDSGVAMKNVSLVIGDTHGKGYKVGEKIVQPIVMDASTSKGKDKQTLN
                     FKAWLVGETEAPDLGQFETLTTFQITYL"
     gene            complement(78921..79406)
                     /gene="sixA"
                     /locus_tag="PN977_000190"
     CDS             complement(78921..79406)
                     /gene="sixA"
                     /locus_tag="PN977_000190"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohistidine phosphatase SixA"
                     /protein_id="HDI6040277.1"
                     /translation="MQVFIMRHGDAALDAASDSVRPLTTNGCDESRLMANWLKGQKVE
                     IERVLVSPFLRAEQTLEEVGDCLNLPSSAEVLPELTPCGDVGLVSAYLQALTNEGVAS
                     VLVISHLPLVGYLVAELCPGETPPMFTTSAIASVTLDESGKGQFNWQMSPCNLKMAKA
                     I"
     gene            complement(79609..81753)
                     /gene="fadJ"
                     /locus_tag="PN977_000191"
     CDS             complement(79609..81753)
                     /gene="fadJ"
                     /locus_tag="PN977_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadJ"
                     /protein_id="HDI6040278.1"
                     /translation="MEMASAFTLNVRLDNIAIITIDVPGEKMNTLKAEFASQVRAIIK
                     QLRENKELRGVVFVSAKPDNFIAGADINMIGNCKTAQEAEVLARQGQQLMAEIHALPV
                     PVIAAIHGACLGGGLELALACHGRVCTDDAKTVLGLPEVQLGLLPGSGGTQRLPRLIG
                     VSTALEMILTGKQLRAKQALKLGLVDDVVPQSILLEAAVELAKQDRPSSRPLPVRERI
                     LAGPLGRALLFKMVGKKTEHKTQGNYPATERILEVVETGLAQGTSSGYDAEARAFGEL
                     AMTPQSQALRNIFFASTEVKKDSGSDAPPAPLNSVGILGGGLMGGGIAYVTACKAGLS
                     VRIKDINPQGINHALKYSWDQLEGKVRRRHLKASERDKQLALISGTTDYCGFAHRDLI
                     IEAVFENLELKQQMVAEVEQNCATHTIFASNTSSLPIGDIAAHAARPEQVIGLHFFSP
                     VEKMPLVEIIPHASTSAQTIATTVKLAKKQGKTPIVVRDKAGFYVNRILAPYINEAIR
                     MLTEGERIEHIDAALVKFGFPVGPIQLLDEVGIDTGTKIIPVLEAAYGERFSAPANVV
                     SSILNDDRKGRKNGRGFYLYGQKGRKSKKQVDPAIYPLIGAQGQGRLSAPQVAERCVM
                     LMLNEAVRCLDEQVIRSVRDGDIGAVFGIGFPPFLGGPFRYIDSLGAGEVVAIMQRLA
                     TQYGSRFTPCERLVEMSKRGESFWKTTATDLQ"
     gene            complement(81753..83063)
                     /gene="fadI"
                     /locus_tag="PN977_000192"
     CDS             complement(81753..83063)
                     /gene="fadI"
                     /locus_tag="PN977_000192"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadI"
                     /protein_id="HDI6040279.1"
                     /translation="MGQVLPLVTRLGDRIAIVSGLRTPFARQATAFHGIPAVDLGKMV
                     VGELLARSEIPAEVIEQLVFGQVVQMPEAPNIAREIVLGTGMNVHTDAYSVSRACATS
                     FQAIANVAESLMAGTIRAGIAGGADSSSVLPIGVSKKLARVLVDVNKARTMSQRLKLF
                     SRLRLRDLMPVPPAVAEYSTGLRMGDTAEQMAKTYGITREQQDALAHRSHQRAAQAWS
                     DGKLKEEVMTAFIPPYKQPLAEDNNIRGNSTLADYAKLRPAFDRKHGTVTAANSTPLT
                     DGAAAVILMTESRAKELGLVPLGYLRSYAFTAIDVWQDMLLGPAWSTPLALERAGLTM
                     SDLTLIDMHEAFAAQTLANIQLLGSERFARDVLGRAHATGEVDDSKFNVLGGSIAYGH
                     PFAATGARMITQTLHELRRRGGGFGLVTACAAGGLGAAMVVEAE"
     gene            complement(83244..83528)
                     /locus_tag="PN977_000193"
     CDS             complement(83244..83528)
                     /locus_tag="PN977_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcZ/YiiS family protein"
                     /protein_id="HDI6040280.1"
                     /translation="MSKCSADETPVCCCMDVGTIMDNSDCTASYSRVFANRAEAEQTL
                     AALTEKARSVESEPCKITPTFTEESDGVRLDIDFTFACEAEMLIFQLGLR"
     gene            83900..85240
                     /gene="fadL"
                     /locus_tag="PN977_000194"
     CDS             83900..85240
                     /gene="fadL"
                     /locus_tag="PN977_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid transporter FadL"
                     /protein_id="HDI6040281.1"
                     /translation="MSQKTLFTKSALAVAVALISTQAWSAGFQLNEFSSSGLGRAYSG
                     EGAIADDAGNVSRNPALITMFDRPTFSAGAVYIDPDVNISGTSPSGRSLKADNIAPTA
                     WVPNMHFVAPINDQFGWGASITSNYGLATEFNDTYAGGSVGGTTDLETMNLNLSGAYR
                     LNNAWSFGLGFNAVYARAKIERFAGDLGQLVAGQIMQSPARQTPQGQALAATANGIDS
                     NTKIAHLNGNQWGFGWNAGILYELDKNNRYALTYRSEVKIDFKGNYSSDLNPAFNNYG
                     LPIPTATGGATQSGYLTLNLPEMWEVSGYNRVDPQWAIHYSLAYTSWSQFQQLKATST
                     SGDTLFQKHEGFKDAYRIALGTTYYYDDNWTFRTGIAFDDSPVPAQNRSISIPDQDRF
                     WLSAGTTYAFNKDASVDVGVSYMHGQSVKINEGPYQFESEGKAWLFGTNFNYAF"
     gene            85607..86854
                     /locus_tag="PN977_000195"
     CDS             85607..86854
                     /locus_tag="PN977_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001530666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6040282.1"
                     /translation="MLPSISINNTSAAYTESINENNNDEVNILVQELKNLFNGKQGIS
                     TCVKHLLALIKNAIRGNDNPDRFNINNSSVTYIDIGSNDTDYITIGIDNQETIKLLAS
                     YKDKELVRAIINDNIVEKTHDDINNADFTDGSIVVSGNEGAIDSNDTADITSGIYNQE
                     PIKSPTNDENKKVGQTIIHENVVENTHDINKKEMIFSALKEIYDGDPGFIFDKISQKL
                     RHTITEFDENGKSEPTDLFTWYGKDKKGDSLAIVIKNKNGNDYLSLGYYDQDDYHIQR
                     GIRINGDSLTQYCSEKARNASAWFESSKAIMAESFATGSDHQVVNELNGERLREPNEV
                     FKRLGRAIRYNFQVDDAKFRRDNVKEIISTLVDNEVDRSQNKYDHFKEIEDKVEKRLQ
                     NRQAKYQNEINQLSALDVNFDDN"
     gene            complement(87033..87788)
                     /gene="mlaA"
                     /locus_tag="PN977_000196"
     CDS             complement(87033..87788)
                     /gene="mlaA"
                     /locus_tag="PN977_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding lipoprotein MlaA"
                     /protein_id="HDI6040283.1"
                     /translation="MKLRLSALALGTTLLVGCASSGTDQQGRSDPLEGFNRTMYNFNF
                     NVLDPYIVRPVAVAWRDYVPQPARNGLSNFTGNLEEPAVMVNYFLQGDPYQGMVHFTR
                     FFLNTILGMGGFIDVAGMANPKLQRTEPHRFGSTLGHYGVGYGPYVQLPFYGSFTLRD
                     DGGDMADGLYPVLSWLTWPMSVGKWTLEGIETRAQLLDSDGLLRQSSDPYIMVREAYF
                     QRHDFIANGGELKPQENPNAQAIQDDLKDIDSE"
     gene            88082..89014
                     /locus_tag="PN977_000197"
     CDS             88082..89014
                     /locus_tag="PN977_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HDI6040284.1"
                     /translation="MDNDKIDQHSDEIEVESEEKERGKKIEIDEDRLPSRAMAIHEHI
                     RQDGEKELERDAMALLWSAIAAGLSMGASLLAKGIFHVELEGVPGSFLLENLGYTFGF
                     IIVIMARQQLFTENTVTAVLPVMQKPTMSNVGLLMRLWGVVLLGNILGTGIAAWAFEY
                     MPIFNEETRDAFVKIGMDVMKNTPSEMFANAIISGWLIATMVWMFPAAGAAKIVVIIL
                     MTWLIALGDTTHIVVGSVEILYLVFNGTLHWSDFIWPFALPTLAGNICGGTFIFALMS
                     HAQIRNDMSNKRKAEARQKAERAENIKKNDKNPA"
     gene            89090..89164
                     /locus_tag="PN977_000198"
     tRNA            89090..89164
                     /locus_tag="PN977_000198"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:89123..89125,aa:Arg,seq:cct)
     gene            complement(89602..90120)
                     /locus_tag="PN977_000199"
     CDS             complement(89602..90120)
                     /locus_tag="PN977_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001406120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HDI6040285.1"
                     /translation="MKFSVVNAVVLLLLIVLTGVFYMKKSKEIAVVNIDAIYNQSAAG
                     DMARKHLNEVKSILEKGYEDAQKVFADNANKEALLLDARQKLERQYVIEENSAKMVLS
                     RAIDNAIGTWRKDNPKNIAVLSSGVVLAHNESADITKEVMTLVEKENLVFNKLPKVEV
                     HNKNEEKPEQSK"
     gene            complement(90180..98092)
                     /locus_tag="PN977_000200"
                     /pseudo
     CDS             complement(90180..98092)
                     /locus_tag="PN977_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001389838.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phase-variable autotransporter adhesin UpaE"
     gene            complement(98117..98737)
                     /locus_tag="PN977_000201"
     CDS             complement(98117..98737)
                     /locus_tag="PN977_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001525246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR family transcriptional regulator"
                     /protein_id="HDI6040286.1"
                     /translation="MVLITTTKNIFIIQAIKSMRWRGFSYVKVIAVDDYSSLIRDFGT
                     SCFRDEQSLLLPVFPDNDSTTCIRSLYFIHQWLKSQPVNIPCLAWGDNGVVSNKGIPS
                     IPWKLTAEQFCFYIDKFINGWRCKKNIRASHKVSFLERQLSPREKSIFIYTKEGKDIA
                     WISNTLGISSKTVWTHRRRAMDKLGVKRLHQFINIPRSLISENIQL"
     gene            complement(99055..99684)
                     /gene="ipuA"
                     /locus_tag="PN977_000202"
     CDS             complement(99055..99684)
                     /gene="ipuA"
                     /locus_tag="PN977_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001181154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type DNA invertase IpuA"
                     /protein_id="HDI6040287.1"
                     /translation="MQNRKFLTHHEINLLLQSVRQKSCSSRDVCMILLAYFHGLRVSE
                     LLSLQLSDLELTTEKIYIQRIKNGFSTVHPLQKEEVIAITNWLNERNSLNVKHFNDNP
                     WLFVSRTGKPLSRQRFYNIVSAAGKNAGLNIKVHPHMLRHACGYSLADNGVDTRLIQD
                     YLGHRNIRHTVIYTASNSMRFEKMWGRGDAKKQHFDPKCKPNLCLEILV"
     gene            100433..101002
                     /gene="ipuB"
                     /locus_tag="PN977_000203"
     CDS             100433..101002
                     /gene="ipuB"
                     /locus_tag="PN977_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type DNA invertase IpuB"
                     /protein_id="HDI6040288.1"
                     /translation="MRKFITHSEWLLFFEAINGSKNEIRDKAMLQMAYVHGLRVSELI
                     ALKISDIDFSESAIYIKRLKNGLSTVHPLQKETVLLLKKWLALRDNIVKKPFEDSLFL
                     SCQGNKISRQYVYKMCKKYSHNMNINIHPHMLRHGCGYALANQGLDTRLIQDYLGHRN
                     IHHTVLYTASNAARFKRVWEGDVLDIKKI"
     gene            complement(101397..102344)
                     /gene="dsdC"
                     /locus_tag="PN977_000204"
     CDS             complement(101397..102344)
                     /gene="dsdC"
                     /locus_tag="PN977_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001314916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator DsdC"
                     /protein_id="HDI6040289.1"
                     /translation="MEPLREIRNRLLNGWQLSKLHTFEVAARHQSFALAAEELSLSPS
                     AVSHRINQLEEELGIQLFVRSHRKVELTHEGKRVYWALKSSLDTLNQEILDIKNQELS
                     GTLTLYSRPSIAQCWLVPALGDFTRRYPSISLTVLTGNDNVNLQRAGIDLAIYFDDAP
                     SAQLAHHFLMDEEILPVCSPEYAQRHALTNQVINLRHCTLLHDRQAWSNDSGTDEWHS
                     WAQHYAVNLPTSSGIGFDRSDLAVIAAMNHIGVAMGRKRLVQKRLASGELVAPFGDMT
                     VKCHQHYYITTLPGRQWPKIEAFITWLREQVSQYECYTL"
     gene            102562..103899
                     /gene="dsdX"
                     /locus_tag="PN977_000205"
     CDS             102562..103899
                     /gene="dsdX"
                     /locus_tag="PN977_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine transporter DsdX"
                     /protein_id="HDI6040290.1"
                     /translation="MHSQIWVVSTLLISIVLIVLTIVKFKFHPFLALLLASFFVGTMM
                     GMGPLDMVNAIESGIGGTLGFLAAVIGLGTILGKMMEVSGAAERIGLTLQRCRWLSAD
                     VIMVLVGLICGITLFVEVGVVLLIPLAFSIAKKTNTSLLKLAIPLCTALMAVHCVVPP
                     HPAALYVANKLGADIGSVIVYGLLVGLMASLIGGPLFLKFLGQRLPFKPVPTEFADLK
                     VRDEKTLPSLGATLFTILLPIALMLVKTIAELNMARESGLYTLLEFIGNPITAMFIAV
                     FVAYYVLGIRQHMSMGTMLTHTENGFGSIANILLIIGAGGAFNAILKSSSLADTLAVI
                     LSNMHMHPILLAWLVALILHAAVGSATVAMMGATAIVAPMLPLYPDISPEIIAIAIGS
                     GAIGCTIVTDSLFWLVKQYCGATLNETFKYYTTATFIASVVALAGTFLLSFII"
     gene            103917..105248
                     /gene="dsdA"
                     /locus_tag="PN977_000206"
     CDS             103917..105248
                     /gene="dsdA"
                     /locus_tag="PN977_000206"
                     /EC_number="4.3.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000426462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine ammonia-lyase"
                     /protein_id="HDI6040291.1"
                     /translation="MENAKMNSLIAQYPLVEDLVALQETTWFNPGTTSLAEGLPYVGL
                     TEQDVQDAHARLSRFAPYLAKAFPETAATGGIIESELVAIPAMQKRLEKEYQQPISGQ
                     LLLKKDSHLPISGSIKARGGIYEVLAHAEKLALEAGLLTLEDDYSKLLSPEFKQFFSQ
                     YSIAVGSTGNLGLSIGIMSARIGFKVTVHMSADARTWKKAKLRSHGVTVVEYEQDYGV
                     AVEEGRKAAQSDPNCFFIDDENSRTLFLGYSVAGQRLKAQFAQQGRIVDADNPLFVYL
                     PCGVGGGPGGVAFGLKLAFGDHVHCFFAEPTHSPCMLLGVHTGLHDQISVQDIGIDNL
                     TAADGLAVGRASGFVGRAMERLLDGFYTLSDQTMYDMLGWLAQEEGIRLEPSALAGMA
                     GPQRVCASVSYQQMHGFSAEQLHNATHLVWATGGGMVPEEEMEQYLAKGRS"
     gene            complement(105353..106891)
                     /gene="emrY"
                     /locus_tag="PN977_000207"
     CDS             complement(105353..106891)
                     /gene="emrY"
                     /locus_tag="PN977_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrY"
                     /protein_id="HDI6040292.1"
                     /translation="MAITKSTPAPLTGGTLWCVTIALSLATFMQMLDSTISNVAIPTI
                     SGFLGASTDEGTWVITSFGVANAIAIPVTGRLAQRIGELRLFLLSVTFFSLSSLMCSL
                     STNLDVLIFFRVVQGLMAGPLIPLSQSLLLRNYPPEKRTFALALWSMTVIIAPICGPI
                     LGGYICDNFSWGWIFLINVPMGIIVLTLCLTLLKGRETETSPVKMNLPGLTLLVLGVG
                     GLQIMLDKGRDLDWFNSSTIIILTVVSVISLISLVIWESTSENPILDLSLFKSRNFTI
                     GIVSITCAYLFYSGAIVLMPQLLQETMGYNAIWAGLAYAPIGIMPLLISPLIGRYGNK
                     IDMRLLVTFSFLMYAVCYYWRSVTFMPTIDFTGIILPQFFQGFAVACFFLPLTTISFS
                     GLPDNKFANASSMSNFFRTLSGSVGTSLTMTLWGRRESLHHSQLTATIDQFNPVFNSS
                     SQIMDKYYGSLSGVLNEINNEITQQSLSISANEIFRMAAIAFILLTVLVWFAKPPFTA
                     KGVG"
     gene            complement(106891..108054)
                     /gene="emrK"
                     /locus_tag="PN977_000208"
     CDS             complement(106891..108054)
                     /gene="emrK"
                     /locus_tag="PN977_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrK"
                     /protein_id="HDI6040293.1"
                     /translation="MEQINSNKKHSDRKKYFALLAVVLFIAFSGAYAYWSMELEDMIS
                     TDDAYVTGNADPISAQVSGSVTVVNHKDTNYVRQGDILVSLDKTDATIALNKAKNNLA
                     NIVRQTNKLYLQDKQYSAEVASARIQYQQSLEDYNRRVPLAKQGVISKETLEHTKDTL
                     ISSKAALNAAIQAYKANKALVMNTPLNRQPQVVEAADATKEAWLALKRTDIKSPVTGY
                     IAQRSVQVGETVSPGQSLMAVVPARQMWVNANFKETQLTDVRIGQSVNIISDLYGENV
                     VFHGRVTGINMGTGNAFSLLPAQNATGNWIKIVQRVPVEVSLDPKELMEHPLRIGLSM
                     TATIDTRNEDIADMPDLASTVTSMPAYTSKALIIDTSPIEKEISNIISHNGQL"
     gene            108470..109084
                     /gene="evgA"
                     /locus_tag="PN977_000209"
     CDS             108470..109084
                     /gene="evgA"
                     /locus_tag="PN977_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system DNA-binding response
                     regulator EvgA"
                     /protein_id="HDI6040294.1"
                     /translation="MNAIIIDDHPLAIAAIRNLLIKNDIEILAELTEGGSAVQRVETL
                     KPDIVIIDVDIPGVNGIQVLETLRKRQYSGIIIIVSAKNDHFYGKHCADAGANGFVSK
                     KEGMNNIIAAIEAAKNGYCYFPFSLNRFVGSLTSDQQKLDSLSKQEISVMRYILDGKD
                     NNDIAEKMFISNKTVSTYKSRLMEKLECKSLMDLYTFAQRNKIG"
     gene            109089..112682
                     /gene="evgS"
                     /locus_tag="PN977_000210"
     CDS             109089..112682
                     /gene="evgS"
                     /locus_tag="PN977_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system histidine kinase EvgS"
                     /protein_id="HDI6040295.1"
                     /translation="MKFLPYIFLLCCGLWSTISFADGDYIEYRGISSNNRVTLDPLRL
                     SNKELRWLASKKNLVIAVHKSQTATLLHTDSQQRIRGINADYLNLLKRALNIKLTLRE
                     YADHQKAMDALEDGEVDIVLSHLVASPPLNDDIAATNPLIITFPALVTTLHDSMRPLT
                     SSKPVNIARVANYPPDEVIHQSFPKATIISFTNLYQALASVSAGQNDYFIGSNIITSS
                     MISRYFTHSLNVVKYYNSPRQYNFLLTRKDSIVLNEVLNRFVDALTNEVRYEVSQNWL
                     DTGNLAFLNKPLELTEHEKQWIKQHPDLKVLENPYSPPYSMTDETGSVRGVMGDILNI
                     ITLQTGLNFSPITVSHNIHAGTQLNPGGWDILPAAIYSEDRENNVSFAEAFITTPYVF
                     VMQKAPDSEQTLKKGMKVAIPYYYELHSQLKEMYPEVEWIKVDNASAAFHKVKEGELD
                     ALVATQLNSRYMIDHYYPNELYHFLIPGVQNASLSFAFPRGEPELKDIINKALNAIPP
                     SEVLRLTEKWIKMPNVTIDTWDLYSEQFYIVTTLSVLLVGSSLLWGFYLLRSVRRRKV
                     IQGDLENQISFRKALSDSLPNPTYVVNWQGNVISHNSAFEHYFTADYYKSAMLPLENS
                     ESPFKDVFSNTHEVTAETKENRTIYTQVFEIDNGIEKRCINHWHTLCNLPASEHAVYI
                     CGWQDITETRDLIHALEVERNKAINATVAKSQFLATMSHEIRTPISSIMGFLELLSGS
                     GLSKEQRVEAISLAYATGQSLLGLIGEILDVDKIESGNYQLQPQWVDIPTLVQNTCHS
                     FGAIAASKSIALSCSSTFPERYLVKIDPQAFKQVLSNLLSNALKFTTEGAVKITTSLG
                     HIDDNHAVIKMTIMDSGSGLSQEEQQQLFKRYSQTSAGRQQTGSGLGLMICKELIKNM
                     QGDLSLESHPGIGTIFTITIPVEITQQVAAIETKAEQPITLPEKLSILIADDHPTNRL
                     LLKRQLNLLGYDVDEATDGVQALHKISMQHYDLLITDVNMPNMDGFELTRKLREQNSS
                     LPIWGLTANAQANEREKGLNCGMNLCLFKPLTLDVLKTHLSQLHQVAHIAPQYRHLDI
                     EALKNNTANDLQLMQEILMTFQHETHKDLPAAFHALEAGDNRTFHQCIHRIHGAANIL
                     NLQKLINISHQLEITPVSDDSKPEILQLLNSVKEHIAELDQEIAVFCQKND"
     gene            complement(112738..113883)
                     /gene="yfdE"
                     /locus_tag="PN977_000211"
     CDS             complement(112738..113883)
                     /gene="yfdE"
                     /locus_tag="PN977_000211"
                     /EC_number="2.8.3.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416872.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA:oxalate CoA-transferase"
                     /protein_id="HDI6040296.1"
                     /translation="MTNNESKGPFEGLLVIDMTHVLNGPFGTQLLCNMGARVIKVEPP
                     GHGDDTRTFGPYVDGQSLYYSFINHGKESVVLDLKNDHDKSIFINMLKQADVLAENFR
                     PGTMEKLGFSWERLQEINPRLIYASSSGFGHTGPLKDAPAYDTIIQAMSGIMMETGYP
                     DAPPVRVGTSLADLCGGVYLFSGIVSALYGREKSQRGAHVDIAMFDATLSFLEHGLMA
                     YIATGKSPQRLGNRHPYMAPFDVFDTQDKPITICCGNDKLFSALCQALELTELVNDPR
                     FSSNILRVQNQAILKQYIERTLKTQAAEVWLARIHEVGVPVAPLLSVAEAINLPQTQA
                     RNMLIEAGGIMMPGNPIKISGCADPHVMPGAATLDQHGEQIRQEFSS"
     gene            complement(113957..114901)
                     /gene="yfdV"
                     /locus_tag="PN977_000212"
     CDS             complement(113957..114901)
                     /gene="yfdV"
                     /locus_tag="PN977_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter YfdV"
                     /protein_id="HDI6040297.1"
                     /translation="MLTFFIGDLLPIIVIMLLGYFSGRRETFSEDQARAFNKLVLNYA
                     LPAALFVSITRANREMIFADTRLTLVSLVVIVGCFFFSWFGCYKFFKRTHAEAAVCAL
                     IAGSPTIGFLGFAVLDPIYGDSVSTGLVVAIISIIVNAITIPIGLYLLNPSSGADGKK
                     NSNLSALISAAKEPVVWAPVLATILVLVGVKIPAAWDPTFNLIAKANSGVAVFAAGLT
                     LAAHKFEFSAEIAYNTFLKLILMPLALLLVGMACHLNSEHLQMMVLAGALPPAFSGII
                     IASRFNVYTRTGTASLAVSVLGFVVTAPLWIYVSRLVS"
     gene            complement(114970..>116474)
                     /gene="oxc"
                     /locus_tag="PN977_000213"
     CDS             complement(114970..>116474)
                     /gene="oxc"
                     /locus_tag="PN977_000213"
                     /EC_number="4.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="oxalyl-CoA decarboxylase"
                     /protein_id="HDI6040298.1"
                     /translation="GFLTQKPGICLTVSAPGFLNGLTALANATVNGFPMIMISGSSDR
                     AIVDLQQGDYEELDQMNAAKPYAKAAFRVNQPQDLGIALARAIRVSVSGRPGGVYLDL
                     PANVLAATMEKDEALTTIVKVENPSPALLPCPKSVASAISLLAKAERPLVILGKGAAY
                     SQADEQLREFIESAQIPFLPMSMAKGILEDTHPLSAATARSFALANADVVMLVGARLN
                     WLLAHGKKGWAADTQFIQLDIEPQEIDSNRPIAVPVVGDIASSMQGMLAELKQNTFTT
                     PLVWRDILNIHKQQNAQKMHEKLSTDTQPLNYFNALSAVRDVLRENQDIYLVNEGANT
                     LDNARNIIDMYKPRRRLDCGTWGVMGIGMGYAIGASVTSGSPVVAIEGDSAFGFSGME
                     IETICRYNLPVTIVIFNNGGIYRGDGVDLSGAGAPSPTDLLHHARYDKLMDAFRGVGY
                     NVTTTDELRHALTTGIQSRKPTIINVVIDPAAGTESGHITKLNPKQVAGN"
BASE COUNT        30028 a        30382 c        27441 g        28623 t
ORIGIN      
        1 cggcagattt ttcataacga atgtcctcga aaccttcgga gcagtcgtgc cattcaaccg
       61 gtgcgtaaag cattgcttca tcaggataaa tcatattgtg tcctttagtc agaaacgcaa
      121 gatctgcgcc agacttgcga ttacgccagc gggattttcc cgatgcgcgt tatgtccggc
      181 gcgaggaatg acatggcatt cggcagccag ttccgccgcc agggcgcgga atttgctgtc
      241 acgttcacca cataaataat aaaacgcaaa tgtgcgggcg ctaaggttag cacgtaaatc
      301 gggctggacg gcgagagaag tcgcctccag catggcggca agcgttgcgc cattattgtt
      361 gctgcgcagc gcaaccagct cccggcgttg ttcgtcattg agtgaggcaa aaacaggctg
      421 ttgataccaa tcagcaaata ccgccgttaa cggttctgtg cgaaaacgct gcgcccattg
      481 gcgatcggaa cgctgacgtt gcgtgcgttg ttcagcattt tgcaacccag gatgcccgcc
      541 ttcgacaaca accccacaaa gccccgccag cccctggcaa gccgccatca tcgccacccg
      601 tccgccaagc gagtacccca ccagccagaa gtcaaggatg ttgtaactaa ccaaggtttt
      661 acacagtaag ccggtgacat catcaaatcc atcgacgcta atcgccgccg aaccaccgtg
      721 accagggaga tcaacataca accgtgagta gtcggcaaac acctcgccca cttcttgcca
      781 ttcatggcaa tcgccggaaa aaccgtgcaa aaacaccagc cagggtaaac ctggtttacc
      841 gtgttttgcc tgcgcgtgca ggatcataaa tggcttacct gcgccagcag ttgctggagg
      901 gtttgcgcac catcggtgtc gttaaccacc atttcaatta ccgtggtggt cggcgtgcgc
      961 caggcgtcga caagtgccgt ttcaagttcc tgccagtttt gcggacgatg atatttcagc
     1021 tcgaacatcg cggcggcgtg ctcaaaatgg acgttttgcg gcatcagata gaaacgctcg
     1081 cgctcgtttt tcggcgttgg caacagcgag aaaatttgcc cgccattgtt gttcaccaca
     1141 attaatacca gcggcgcgga aacctgacgt aataacgcca gcgcgttgag atcgtaaagt
     1201 gctgagagat cgcccacaat cgccagcgtc ggtttgccac tggcccgctg aacgccagcg
     1261 gcggtcgaga gcagcccgtc gataccgctg gcacctcggt tgctgtacac cgggtaacct
     1321 gccggaagtt gcgaaagcgc atcaatcaga cgtactacca ggctgttacc gacaaacaat
     1381 tgcccctgtt caggcagata gtcgctgatg cgatgcgcca gttgggcttc gccaaacgca
     1441 tcgcgacggg caataaccgc ctgcattgcc tgttccgcca ggcgcgggat ttcaacgcac
     1501 cagggctggc gtttttctgc cggatgttgc tccagccagt cggcaatatt ggcaattaag
     1561 cgacgtccgc gatggtgtgc cggatcaagt cgcccttcaa tgtcatcaac aatccagtac
     1621 tcttcgggtt cacagcttgc ctgccattgc aggagccgtt tgcccgtcag gctgcttccc
     1681 agttgcacca caatttgcgc ctgttgcagt tcgctggtcg ctttggcatt gcctaaccag
     1741 agatcggcac acggcagcgg ctgtccggtt tgcgacagca catcgccaat cagcggccag
     1801 ccaagagttt gcgcccacag tgcgactttt ttgccctctt ccgcactcat gcgcccggca
     1861 accaccacgc cgcgcttttg tcgccagaag aaccagtcgc gctgtttttc actttccaga
     1921 cgaggcgctt cacgcagcca cggcttatcg tcctgccacc agtcgcccag acgctgttgc
     1981 cagctaagcc cggtgtcgtc catttcgcca tacagcggtt cagcaaacgg gcagttgata
     2041 tggactccgc ccgcatgaag cgtaccgaga gcatggtcga tggtagaaac cagccaacgt
     2101 gcggggatat cctgggtcgg gcgcggcagt gaaatactgt gcgtggggtg agaggcgaac
     2161 attcccggct gacgaattgc ctgattcgcg ccgcagtcaa tgagctccgg cgggcgatcg
     2221 gcggttaaca ggatcagttt ttctccggtt aacccggctt caatcagtgc cggatagaga
     2281 tttgctaccg ccgtgccgga ggtcacaatc accgccaccg gctgcttgct gactttcgcc
     2341 agccccagcg ccagatgccc cagtccacgc tcatcgaaat gggtgtggtg aatgaatgcg
     2401 gaattctccg ccgccgctaa cgttaacggt gtagaacgcg agccaggggc gatacagatg
     2461 tgtctgacgc cttgacgcgt taatgcttcc agaatgaccg ccgcccagcg tcggttaaat
     2521 gcgcttactg acatgagttt gtccggtatc aatattgcgg ctaagtataa ggagctacaa
     2581 aaatagatta ttgatatgaa tcggtaatga tgcgactcat tactattcca tttgtaataa
     2641 agtacgcagc cctgccgctt tgttgtcgat ttcctgccac tcttgctcgg ggtcggaacc
     2701 acggacaatg cccgcgccag catatagacg cacgacattg ccgctaattt ttgctgagcg
     2761 cagggaaacg cagaattcgc tttgttgtaa tgagagatag cccgcagaac cggcgtacca
     2821 ttctcgggtg aacggttcgt gacgggcgat aaactgtcgc gccagatcgc gcggtaagcc
     2881 agcaactgct gccgttggct gcaactgatg taaacagatc acatcatccg ctttgttgag
     2941 tgcagtccag atacagcggc gaagatgctg cactttacgc agacgcagta cctgcggcgg
     3001 taaaacatcc aacgtctggg tatcggcctg caatcgttgg cagatatcct ccaccaccaa
     3061 catgttctcg cgctggtttt tatcatccgc catcagccac tcacccaacc gctgcgcctg
     3121 cttatcatca ggatgatttg ctaccgttcc cgccagcgct tcagtacgca gcgctttgtc
     3181 acgccgccac cataatcgtt ccggtgaaga gccaagaaaa gcattttcgc catcaaaggc
     3241 catgtaaaaa tggtagcaat tcagattcag tcgacgactg gcagccatca tcgccgccgc
     3301 attgaccgga cttgcgaaat gcaggtcagt tgcccgagca agcaccactt tgtcgagctc
     3361 gccttcggcg atggttttcg ttgccagttc gattaattgc gtccagcccg ttttgtccgg
     3421 ccagtgttgt tctcgggtgg tggttaaatg taacccaggc aagggtttaa tactcaccag
     3481 tgtggcgata aattcttttg ccttaatcgc atcatgctga agggatcttt cgctgaatag
     3541 cgtcagccgc agcgtggctt taccgccaca gcgtcgccat tcgaggcgtg gtaaaagtaa
     3601 attgccctgc gacgggtcaa acgcattcaa cccccagatg cgtaagtcgg cgtgttccgg
     3661 gtgctggcga agaaaacgtt gtgcctggtc caacgacgta aaacgggtta tcgcacccag
     3721 gacggcagct tcttcatcac cattacgttg ttgccagtag aattgcggat acacctgctg
     3781 actggccagc cagttcaagg catcaaaagc gtctttgaga gggaaaggaa tatcgataac
     3841 ccgaatgccg ggtgtcgccg gaatctcttg cgacaaatgg cgcagtagat tttccagcgc
     3901 cgtagtaagt gattgcacgc ggacctctcc ctgttaaaag cctcacatta tacggggtac
     3961 gacaaaaaaa atgcagtacc ccggtgtagg gaggttttaa cggcgtgcca gcaacagtcc
     4021 tagtaccagc ccaacggccg cacccacacc aattccttgc cagggttttt cgtggacgta
     4081 gtcatcagca cgataaactg cctgcttcgc ccgatagtaa taactgtctg aagcctggct
     4141 aacccgtttt ttcacatcat ccagcgcttt ttctgcacgc gctttcagct caacatattt
     4201 ctgatcggcg ggatcgccag aggagcggag cacctcttcc agtgtttcac taagcagcgt
     4261 caggtcgtca tcgatacgtg tatcaccaaa ctgattagac attctcgttc tccatttgcg
     4321 taaaacctgt cactaactat agacaatggt tattgattac gcctgaatta cctcgcgtgc
     4381 catcccaatg tgtgggatac catcttcttc atagacctcc gtcacaggga taaagccaaa
     4441 actttggtag aagttttgca gatgcgcctg cgccccgagg taaacaggct tatcaggcca
     4501 gtggtgcgcg catgtttcga gtgttttact catcagctgc tgccccactt tttcgccacg
     4561 caacgcttca ctgacaatca cccgaccaat aacgaccggt tcaagatcat catcactttt
     4621 cagaatcctc gcatacgcca ccagttcatc atttttccaa ccgagaatat ggcgattatc
     4681 cctcgtcaaa tcatcaccat cgatatcctg ataagggcaa ttctgctcaa caacaaacac
     4741 cgcgcaacgc agttgtaata aggcataaag ctgggaaacc gataattcag agtgatgcag
     4801 atcttgccat tcaatcataa tgctctcctt ttagatgtat gccttatact agccggaata
     4861 ttttttgaaa cgtgatgaac tcatggaatt aattttttta ggtacttcag ccggtgtgcc
     4921 aacccgtacg cgcaatgtca cggcaatatt gcttaacctg caacatccga cccagagcgg
     4981 tctttggttg tttgactgcg gtgaaggcac ccagcatcag ctactgcata ccgcctttaa
     5041 ccctggaaaa ctggacaaga tttttatcag tcaccttcat ggcgatcatc tctttggttt
     5101 acccggcttg ctgtgcagtc gttctatgtc tggtattgtc caacccttaa cgatttatgg
     5161 tccgcaaggt atccgtgaat ttgtggagac cgcgctgcgg attagcggct catggaccga
     5221 ttatccgctg gaaattgtcg aaattggcgc tggcgaaatt ttcgatgatg gcctgcgcaa
     5281 agtgaccgct tatccgctgg aacacccact ggaatgctat ggctatcgta ttgaagaaca
     5341 tgataaaccg ggtgcattaa atgcccaggc attaaaagct gctggcgtac cgcctggccc
     5401 actgtttcag gcattaaaag cgggcaaaac aatcatgctg gaagatggaa ggcagattaa
     5461 cggcgcagat tacttagccg ctccagtgcc aggtaaagcg ctcgctattt tcggcgatac
     5521 cggcccctgc gatgccgcac ttgacctggc taaaggtgtc aatgtcatgg tgcacgaagc
     5581 gacgctggat ataaccatgg aagccaaagc caatagtcgc ggccacagct ctacacgcca
     5641 ggctgcgaca ctggcccgtg aggctggagt cggtaagcta atcattaccc acgtcagctc
     5701 gcgctatgat gacaaaggtt gtcagcacct gttacgtgaa tgtcggtcaa ttttcccggc
     5761 gactgaactg gcgaatgatt tcaccgtgtt taacgtttaa ctctgcttat tattaaaaac
     5821 agggctaaac ttgccctgtt atcgcaaccc acgccacata tatccaccca cacgggcata
     5881 ttgagcgatt tttgatattt attcatctcc ttacgcacta tattcagagc aataagttgc
     5941 ctctttttat cttcattcag cacaaccttt gcaactaaaa ggagcctata atggttacag
     6001 ttgtcagcaa ttattgtcaa ttatctcaaa cccaactcag tcagacattt gcagaaaaat
     6061 ttactgtgac cgatgaatta ctgcagtctt taaaaaaaac agcgttatcc ggagatgaag
     6121 aaagtattga gttactgcat aacattgcgt taggttatga tgaatttggg aaaaaagctg
     6181 aagatattct ttaccatatt gttagaaacc caacaaatga gaccctatcg attatccgac
     6241 ttattaaaaa tgcctgttta aaattatata atttggcaca taccgcaacc aaacaccccc
     6301 tcaaatcaca tgattcagat gatctcttgt tcaaaaaact attctcccct tcgaaattaa
     6361 tgacaattat cggtgaggac attcctctta tatcagaaaa acagtcgctt tcaaaggtac
     6421 ttttaaatga taagaataat gaactgagcg atgggacaaa cttctgggat aaaaatcgtc
     6481 aattaaccac agatgaaata gcttgctatc ttaaaaagat agccgctaat gcaaaaaata
     6541 ctcaagtcaa ttatcctact gatttctacc tccccgattc caacagcact tacctggaaa
     6601 tcgctctcaa tgataatatt aagagcgatc catcatggcc gaaagcagtc cagttattcc
     6661 ccataaatac tggcggacac tggatattag tttcgctaca aaaaatagtt aatgaaaaaa
     6721 ataacacaca acaaataaaa tgcatcatat tcaattcatt acgtgcacta ggccatgaaa
     6781 aagaaaattc acttaagcgc atcattaata gttttaattc tgaactcatg ggagaaatgt
     6841 cgaataataa tataaaagtt catttaactg aacccgagat aatattttta catgccgatc
     6901 ttcagcaata cttaagccaa agttgcggtg catttgtgtg catgtcagcc aaagaagtaa
     6961 ttgaacaaag ggaaagcaat tctgacagcg ctccctatac gttattaaaa aactatgctg
     7021 acagatttaa aaaatattca gcagaagagc agtacgaaat tgattttcaa catcgacagg
     7081 taaacagaaa ttgttattta gataaatatg gcgatgcaaa tatcaatgat tattatagag
     7141 acttagaaat aaaacactca caaccccaaa atagagcatc aggcaaaaga gtaagttaac
     7201 atcattgtta attaattcga ccagtcagca aatatgctga ctggtcgaac atagtttgct
     7261 aaaaagtcat cactgactga tgtcagtcac accacccgcc agttcaacca ggcgaataaa
     7321 ttccgccctg taaccctgtg gatcttcccc ttttgcctgc tgagcccact gttttatctg
     7381 ctgccatgag atttcatcca ggtattcaga accgcgtaac ttttgcccat atgcagctac
     7441 tgctgcgcga aaacgcatat cttcagaggg cgcgtttatt gttggcccca gcgggaattc
     7501 aactaactga ctttcttttc cctgcgggta tttccaacga atctttaacc aggcaagttc
     7561 ttttgttttg tccgatttcg ctgatttgtt atccggggca tagcgtaact tatcaattga
     7621 tgctttttgc cctttcagcg ttaattcgaa taacaacgtt atatgtttac ctgcgccaat
     7681 atcacccgca tcaacgttgt cgttattaaa atcttccgcc cgaagttgac gcttttcata
     7741 accaatctga cggtattccg ttacccacgc ggggttaaac tcaatttgcg ctttgacatc
     7801 ttttgctacg ctaatcaacg tctgccgcat ttcactgttc aatactttct gtgcttcagc
     7861 gagggtatca atgtagctgt agttaccgtt gccaacatcg gcgattcgca ccatcattgc
     7921 ctcgttgtaa ttgctatccc ccacgccaaa cgtggacaga gaaacaccgg actcccgctg
     7981 ttttttgacc attgattcaa tcgatttagg atcgtcaatg ccgacgttaa aatcaccatc
     8041 agtggccaat aaaatgcgat tgatgccacc cttaataaac cccttcgccg cctgctgata
     8101 agccagttcc agcccggcac cgccattggt actgccatcg gcatccagcg aatcaattgc
     8161 ggcattaatt tccgccttgt gactcccgga gatagaaggc aacgcaatac gggagtcgcc
     8221 agcataggtc acgatggcaa tgttatcctg ctcacgaagt tctttgacca ataatttcaa
     8281 cgaagactgg ataagtggca aacgttcatc agaaatcatt gaaccagaag tgtcgataag
     8341 aaagaccaga ttagaagctg gtaactcttc acttttgcga tctttcgcca ggatatcaac
     8401 tttcagcaat gttcgctgtt cattccacgg cgcaggtgcc agttcgtagc gcatagcgaa
     8461 aggtattggc ttactggcgg gaacaggttc tttattatta cttttatcat taataaccca
     8521 gtctgacgga aaataattca ccatctcctc cacccgcact gcatctggcg gaggcaactg
     8581 cccatgattg agaaaacggc gtacattcgc ataactgccg gtgtcgacgt caaggctaaa
     8641 tgtcgccaac gggttttgcg ctacctgctt aaccggatta tcatcgaact gctgatagcg
     8701 agcggttcct ggatttgcga tttgggaggc tgctttttct ctggctgcat gttgatattt
     8761 tggagccgcc tgtaagcgcc cctgcaaagc ctgtttgtct gaatattgtt gcgcttcttg
     8821 ctgggctaag gcttttgctt tcgcgtctgc agtggcttta gcggcgacgg atctttgctc
     8881 ggcctctttt attgcagcat gttgcgcggc aagcacttgc tggtccgtgg gagtactggg
     8941 ttgttgttgc agactttcct tattctcaga ttccggccca catcctgaca aaatcaaact
     9001 actcataagc aacatgatta tatttttatt tcgcatataa aatcctttta aataaactca
     9061 ggcaccagac ataaaataca ctctttgtat catcctgctg atcttgataa aatacaatat
     9121 cgaaacctat aacaaataat gattcaataa cgcaacccat taataatttg catatgaaaa
     9181 aaaataaact ttgcttttat tatattaatt ctgttttcac tccctctgat tattttttac
     9241 caaccctggg tcaatgcatt accgccaacg ccccgacacg caagtcctga gcaattagaa
     9301 aaaacagttc gttatcttac acaaactgtg catccccgta gtgccgacaa tattgataat
     9361 ctgaaaaggt ccgcagagta tataaaagag gtctttgtca gtagcggtgc cagagttacc
     9421 tcgcaggacg cccccattac gggtggcccc tacaaaaaca ttgttgctga ttatggtcct
     9481 gccgatggac cgctgattat tattggtgcg cactatgact ctgccagcag ttatgaaaac
     9541 gatcaattga cctatacgcc gggcgcggat gataacgcca gtggcgtggc aggattactc
     9601 gaactggcac gtttgttaca gcagcaagca ccgaaaactg gcgtgcagct ggtcgcctat
     9661 gcgtcggaag aaccgccctt ctttcgtagc gatgaaatgg ggagcgcggt acatgcagct
     9721 tcgcttgagc gtccagttag attaatgata gcgctggaga tgattggcta ttacgactct
     9781 gcgcctggta gtcaggatta cccttacccg gcaatgtcct ggctttatcc cgatcgggga
     9841 gactttattg ccgtggtcgg cagaatgcag gatatcaacg ccgttcgtca gctaaaagcg
     9901 gcattgttgt catctcagga tttatctgtt tattctatga atgccccagg gtttattccc
     9961 ggcattgatt tctctgacca ccttaattat tggcaacacg atattcccgc cgtaatgatt
    10021 accgacaccg ctttttatcg taataaacaa taccacttgc ccggtgatac cgcagacaga
    10081 ttgaattatc agaaaatggc ccaggtcgtg gatggcgtta tcaccttgtt atacaacagt
    10141 aaacaataaa attttctcga ggcgggtcca gaaaaagcaa catgcgcatt ttattttccc
    10201 tcatctgttt taagatgcga aacatgaaat tcaacggagg aaaaataaat gggaatgatt
    10261 ggctattttg cggaaatcga ctcagaaaag attaaccaac tgcttgagtc agctgaaaaa
    10321 cctttaatgg acaatattca tgacacgctt tcaggattac gccgactgga tattgataaa
    10381 cgctgggatt ttttacattt tggcctgaca ggcacctctg cgttcgatcc agcgaagaat
    10441 gatccgttga gtcgtgccgt gcttggtgaa cacagcctcg aagatggcat agatggtttc
    10501 ctggggttga cctggaatca ggagttagct gcgacaatcg accgcctgga agctcttgac
    10561 cgaaatgtgc tgcgtaaaca gttttccatt cagcgactca atgaaatgga gatttatccg
    10621 ggagtaacct tttcagaaga gttagagggg caactctttg catccatcat gcttgatatg
    10681 gaaaaattaa ttagcgctta tcggcgtata ctgcgacagg ggaatcatgc gctgacggtt
    10741 attgttgggt gatggggtca gggcaccaaa aaaaaaccgc cgaacatgtc ggcggctttc
    10801 tgaattacga actgacagct tacatcagcg gcatggctaa ccgcacaatg ctaatcagcg
    10861 gttgtggcca tacacccagc accagtacca acagtgcaga gatcagcacc acgataccgc
    10921 ccgcgctgta ctgccagttt gacggcgcat cacgacccgg ttgttccggg gcgtgaagat
    10981 acaggctcac cgccacgcgc aggtagtagt agaggccgat tgccgaaccg acaaccacgg
    11041 cgcccaccag ccaccacaag tgtgcctgaa caccgactgc cagcacgtag aacttaccga
    11101 taaagcccag cgtcatcgga ataccggcca gagacagcat catcaccgtc atcactgccg
    11161 cgaggatcgg acgatgccag aacagaccgc ggtaagagaa cagggaatca gcatccgggc
    11221 cacgatacgg gctggacatc aggctgacca cgccgaacgc gccgaggctg ctgaacagat
    11281 aaccggccag gtaaacccct accgcttcca tcgacatctc gcccgtttgc agcgcaatca
    11341 gcgccaccag cagatagccg aggtgagaga tagatgagta accgagcaga cgtttgatat
    11401 tggtctggct cagcgccatc aggttaccga agatgatgga ggcaaaggcg ataatcgcca
    11461 gcaccacccg aatcgcttcg ctgtcaccca ccggtgcgta gaggaacaga cgcatcacca
    11521 caccgaagat agcgattttg ctcgccgtcg ccaggaaagt ggaaaccggc gcaggcgcgc
    11581 cctggtatac gtctggcgtc cacaggtgga acggcaccag agagagtttg aagccgaggc
    11641 caacaatcat caggccgaaa cctgccagca acagcggctc gttgagcata ccgtcgccga
    11701 ggtttttacc cagcgcgaca aacgacaggt cgccagactg cgcatacacc agcgccatac
    11761 cgaacagcag gaaagaagac gctgcggcag agagaatggt gtatttgata ctggcttcca
    11821 gcgaacgttt ctggcggaaa gcgtaaccga ccaggccaaa cagcggcaaa gagatcagtt
    11881 cgataccgag gaacagagac gccagatggt tggcattcgc cagcaggatc ccgcccaggg
    11941 cggcaattaa caccaacagg tagaactcgt ccttgttgtc gttatagcct tcaagccacg
    12001 ggtaggcgaa agtacaggtg gcgaggctcg ccaacaatac cagcccggtg taaagcatgg
    12061 cgaaaccatc aacgcgcatc agcggcgtaa cgtccatagc gcccgcctgg ccgacaaacc
    12121 agagcgaaac cagcgccgcg ttaagcccaa taaccgagag cgtagcgttg aggaaatgat
    12181 tgcgtcgcca cgcaatggag agcatcacaa ccaccaccgt caagccgacg atcagcaacg
    12241 gtagcagtgc gatcaggttt tgtggagtta ttgtcatggc gatttacggc cttgtagtag
    12301 taacggaatt aacaaaccac tgctggatat tgccaatcgc ggagtgcgag gtatccagaa
    12361 tcggctgcgg atagaagccc agcagtacca gcagcaccac cagcaacagg atcataaaca
    12421 gctcacgcag cgacatccct ggcagttcct ggctggcaat ctggcttttc gctttaccga
    12481 agtaagcgcg atgcaacatc gccagcgaat aaacagatgc aaagaccagc ccaaaggtag
    12541 agataacggt aatcaccggg acaacctgga agctgccgaa cagaatcata aattcgccga
    12601 cgaagttacc ggtgcctggc atcccaagcg ttgccaccgc aaagaacagc gacagtgctg
    12661 gcagccattt catcttgctc cacagaccgc ccatcatgcg catgtcgcgg gtatggatac
    12721 gttcataaag ctgaccacaa agaataaaca gacccgccgc tgacaagccg tgcgcaatca
    12781 tctggattac cgcgccctgg taggccaact ggctgccggt atagatagca atcagcacga
    12841 agcccatgtg ggaaaccgag gtgtaggcga tcagacgttt gatatcggtc tgggcgaagg
    12901 ccatccacgc accgtagaag atgccgataa cacccagcca catagcgatt ggcgcgaact
    12961 ctgccgacgc gttcgggaac agcggcaggg agaaacgcag cagaccgtaa gcagcggttt
    13021 tcagcaagat ccccgcgagg tccacggaac cggcggtcgg agcctgtgag tgcgcatccg
    13081 gcagccagcc atgcagcggt accaccggca ttttcaccgc aaacgcgatg aagaagccca
    13141 gcatcaacag atattccaca ccactggaca ttggcgtatt caacagctct tcatagttga
    13201 aggtccagac gccagtcgca ttgtagtgaa caaataccag cgccaggatg gcgatcaaca
    13261 tcaccagacc actcgcctgg gtgtaaatga agaacttggt tgccgccgtg atacgcgttt
    13321 taccgtcaga ggctttatgc ccccacagtg cgatcaggaa gtacatcggc accagcatca
    13381 tttcccagaa gaagaagaac aggaacatgt cgatggcaag gaacacgccg ataacgccgc
    13441 ccaggatcca catcaggttg aggtggaaga agccctggta tttttcgatc tctttccacg
    13501 aacagagtac cgccagcaca ccgagcagac cggtcagcac gaccatcagc agcgatagcc
    13561 cgtcaatggc gagatggata gagataccaa aacgcgggat ccacggcatg tcgaattcag
    13621 actgccactg cggaattccg gcggattgcg tcagtgaata accgccctgc aaccacagtt
    13681 gcagcgacag cgccagcgtc aatcccatgg tgatcagcgc gatccagcgc ggcaccttga
    13741 cgccaaagcg ttcggtctgc cagcacagga agccgccgat aaagggaatt aatattagcc
    13801 agggtagtaa catggcgatc tttattcctt gtaaaagtcc cgtcaggacc ggattttcaa
    13861 cgaattctca cgacagaaaa ttctcttcgg actgggggta gtgtcggatg caccgcgaat
    13921 gcggcttatc cgacctacgt tcttatatgc ccaggggcaa tcccacaatc cttaactcaa
    13981 cgcagtacca tcaacagtgc cagcaccacg accgcaccga tgctcatgga tgccacatac
    14041 cagcgcagat aaccgttctc gcttaacagc agacctttac ctgcaaagcg ggaaaggaca
    14101 gccgggatgt tcatcattga gttcagcgga tcgcgtttca gcaaccaggc aatacccagg
    14161 aacggcttga cgaacacttt gtcgtacagc cagtcaaagc cccaggcgtt gtaccaccag
    14221 gtgcccagca gacggcccgg cgcactgttg gcgatggagg tcaccagagt acgtttaccc
    14281 agccacagcc aggcagccag cagaatgccg accaccgcga ccacgccaga agtaatttcc
    14341 agggtcaaca tgctgccgtg cgccagctcc gtcgtttgcg gaagcacgcc ctgcagcggc
    14401 ggtacaatca gtgcgccaac gaaggtggaa aggatcagca gcacaatcag cggcaggctg
    14461 tgagttaccc ctttcacggc gtgagcgtga atttgttctt ttccgtggaa gacgatgaaa
    14521 atcatacgga aggtgtaaag cgaggtcata aacgcaccga ccagacctgc caccatcaga
    14581 ttgatatgtc cattcgccat cgcacccgcg aggatctcat ccttactgaa gaagcccgca
    14641 gtgaccagcg gcagtgccga cagtgctgcg ccgcccacca ggaagcagag ataaaccagc
    14701 ggaatagatt tacgcagacc gcccatcttg aagatgttct gttcgtgatg gcaggccaga
    14761 atgacggaac cggatgccag gaacagcagc gctttaaaga acgcgtgagt catcaagtgg
    14821 aaaatcgccg catcccatgc ctgcacgcca agcgcgagga acatgtagcc aatctggctc
    14881 atggtagagt aagcgagaac acgtttgata tcggtctgta ccagcgcggc aaaaccggcc
    14941 agcagcagcg taaccgcccc aacaatgccc accagatgca gaacttccgg cgtcatcagg
    15001 aacaggccgt gggtacgggc gatcaggtag acacccgcgg ttaccatcgt tgcggcgtgg
    15061 atcagcgcgg agacaggcgt cgggcccgcc atcgcgtcgg caagccatgt ctgcaacggc
    15121 aactgcgcag atttaccgac cgcaccgccc agcagcatca gcgtcgccca catcagcatg
    15181 ttattgccat cagcaaagtg cgctggtgcc agttccacca tttcgcggaa gttcagggtg
    15241 cccagttcgt tgtagagaat gaacagcgcg aaagcgagga acacgtcacc cacacgggtc
    15301 acgacgaacg ctttcattgc cgctgcgcca ttcttcggat cggtgtaata gaacccgatc
    15361 agcagatagg agcacaggcc cacgccttcc cagccgaggt acatcagcag caggttgtcg
    15421 gcaagaacca gaactaccat gctggcgatg aacaggttgg tgtaagcgaa gaagcgagag
    15481 tagccctctt caccgcgcat ataccaggag gcgtacatgt gaataaggaa acccacacca
    15541 gtgaccaccg agagcatggt cagcgacagg ccgtccagca ccaggttaaa accgatgtta
    15601 aagtcgccta ccgacatcca cgtccacagc ggctggctgt atgcctgctc accgttagcg
    15661 aagaagtcaa cgccgataaa ggcagttacc agcgccgcca ggcccacaga gccaacgccc
    15721 acgatcgccg agacgttttc agaccagcgc ccacgggaga acgccagcag gacaaagcca
    15781 atcaatggca aaataatggt taaggcaagc atgttcatcc gcgcatctca cttactgaat
    15841 cgatgttcag gttctggcga cgacggtgaa gttgcagcag cagcgcaagg ccgatacttg
    15901 cttctgccgc cgcgaggctg atggcgagaa tgtacatcac ctgaccgtcg gtctggcccc
    15961 agtagcttcc ggcgaccaca aaggccagcg cggaggcgtt aatcatgatt tccagaccaa
    16021 tcagcataaa cagcagattg cgacggataa ccagaccggt taagccaaga acgaataaga
    16081 ttgccgcgag gatcagtcca tgttgtaagg ggatcatgcg tgctcctccg tttttctttt
    16141 cgcgctgtcg tctttacgat tgctcagcac ttcacccgca cgctcttcac gaccaacgtg
    16201 gaaggccaca accagacccg cgagcagcag catagaagcc agttccaccg ccagtacgta
    16261 aggcccgaac agcgtaatac ccactgcttt agcactgatt ggcgtaccgt cgataccctg
    16321 atcattaaca ccaaggatgg cgtaaacaat caccaccagc atgatggccg acaaaatcgc
    16381 cggaccaatc cacacctgcg gtttcagcca ctggcgttcc tgttcgattt ctgaaccgcc
    16441 caggttgagc atcatcacca cgaacacgaa cagcaccata atggcacccg cgtagacgat
    16501 aatttccagc gcaccggcga agtaagcgcc cagtgagaag aacaccccag aaatcgccag
    16561 cagcgaaata atcaggtaca gcagtgcgtg taccggattg gtatgggtga tcactcgcaa
    16621 ggtcgcaagt atggctatca ggccacagat ataaaaagcg aactccattg cccctctcct
    16681 tacggtaaca ggctcttgac gtcgataggc ttggcttcgt tctctgcttc acccttatct
    16741 ttgccgtcga ttgccatacc tgccatccgg tagaagttat attccgggta tttgcccgga
    16801 ccggagatca gcagatcctc tttttcgtaa accagatcct ggcgcttgta ttcccccatt
    16861 tcgaaatccg gggttaactg aatcgccgtg gtcggacagg cttcttcgca cagaccacag
    16921 aaaatgcagc gtgagaagtt gatgcggaaa aattccggat accagcgacc gtctttggtt
    16981 tctgcctttt gcagcgagat acagccgacc gggcaggcta ccgcacagag gttacaggct
    17041 acgcaacgct cttcgccgtc cgggtcgcgg gtcagaacga tacgaccacg ataacggggc
    17101 ggcagataga ccggctcttc cgggtacatt cgcgtttcgc gtttggcgaa cgcgtgcagg
    17161 ccgatcatcc agatactacg aacctgagtg ccgaaaccta ctaacaattc ttttaaggtc
    17221 atggtcttat tgccccttat tgcgcctgcc agagaatgac agccgccgtt accagcaagt
    17281 tgatcagcgt cagcggcagg cagattttcc agccgaagga cattacctgg tcataacgcg
    17341 gacgcggtaa cgacgcacga atcaaaatga acatcatcat aaagaacgcg gttttcagcg
    17401 cgaaccagat gaatggcggt aacaacgggc cttgccagcc accgaagaac agcgtcacca
    17461 tcaatgcaga gatggtcaca atcccgatgt attcgcccac gaagaacaga ccgaacttca
    17521 taccggaata ttcaatgtgg taaccatccg ccagttcctg ctcggcttcc ggctggtcaa
    17581 acgggtgacg gtgacacacc gccacgcccg cgatggcaaa ggtaataaaa ccaaagaatt
    17641 gcgggatgac gttccacacg tgcgcctggc tgttgacgat gtcggtcatg ttgaatgaac
    17701 cggcctgcgc caccacgccc atcagggaaa gcccgaggaa cacttcgtag ctcagggtct
    17761 gcgcagaagc acgcatcgcc cccagcaacg agtatttgtt gttacttgac cagcccgcaa
    17821 acagcaccgc gtagaccgcc agaccggcca tcatcaggaa gaacaaaatc ccgatgttca
    17881 ggtcggcaac cacccaacct gggctgactg gcacaatcgc aaaggccagc agcagcgagg
    17941 taaaggcaat catcggtgcc agggtaaaga tgacgcgatc cgagaatttc gggatccagt
    18001 cttctttaaa gaacatcttg atcatgtccg cgaccagctg gagcgaaccg ccccagccaa
    18061 cacggttagg tccgtaacgg ttctggaaca gacccagcag gcgacgttcg ccaaagctca
    18121 tgaatgcccc acaggtgact accaccagca ggatcaccac cgctttgagg atggtcagca
    18181 ggatctcaat cagttccggt gatatccaac tcattgttgt gcctccttga gatcctcaag
    18241 atgcgcgcca gccagcaccg gagcaatacc ggacatgccc atcggcaagc ccacctgccc
    18301 tgccgtcagt ccttcggcga tttcaaccgg cagcgtgacc gtgttgccat cgtaactaaa
    18361 ggagacgcgt gtacctgcgt tcacacccaa cttcgcggca tccgctgggt tgagtttgat
    18421 gtacggctgc ggcatacggc tctggaagac cggagcacgc tgtgacaatt catcgctgcc
    18481 aaacaggtgg taatacggcg cgatacgcca tttcccgtcc tgcggctgga agcgtgccgg
    18541 tacgctggtg aagtaatcca gaccattttc gctggtttca aacagacgca cgcccggatc
    18601 gccaaagcgc agtttgccgc ccacttcgtc ctggaatttg ttccacgcct gcggggagtt
    18661 ccagcccggt gcccaggcaa acggcacttg cgaacggtgc gcggtcggct ggttgttacc
    18721 ttccatcgag aaggtgaaca tggtgtcaat atcctgcggc tgacgcggct catgaacgct
    18781 gatattggcg cgcatggcgg tacgaccgct gtaacggtgc ggctcacggg ccagtttctg
    18841 cccacgaata cggaatgtcg catccggcgc agcatctttg atacccgcca gttccgggat
    18901 tttcgccaca acagcgtcaa tcacatggtc gagctgcgtc cagtccactt cacggctcag
    18961 cagggtgcta tgcagcgagt gcagccagcg ccagctttcc agcatgacag ttttgctgtc
    19021 gtaataagcc ggatcgtaaa cctggaagaa acgttgggca cggccttcgt tgttaatcac
    19081 cgtaccgtcg ctttcagcaa agctggcagc agaaagtacc agatgggcgt tttccataat
    19141 cgctgtgcgt tgatggtcaa ccaccatcac cagcggcgct ttagccagcg cagcattcac
    19201 gcgggtagca gaagcgtgac gatgcagatc gttttccaac accaccaccg cgtcggcgcg
    19261 tccggtttcc agttcggtta acgcttcttc aagcgaaccg ccacccataa tgcccagccc
    19321 catgctgttg acggaacggg caatcatggt gataccaacg tcagcgccgc gacctttcag
    19381 ggctttcgcc acgttagccg ccgcctgaat cacttcagcg ctaccagcgt tcgtcccgga
    19441 gataatcaac ggtttcttcg caccagccag tgcctgcacg atgacgtcga ttttgctttg
    19501 cagctcaggt tcgataccgt caaccgctgg cgcggagtta tccagcgcat gggcgatggc
    19561 aaaacctaaa cgcgcctgat cttcaaccgg tgcgcggtaa gtccacgccg cgatatcatc
    19621 cagacgggtg tcatcaacgt tggtaacaaa cagcggatgc ttagcacgtt gaccgatgtt
    19681 gaggattgcc gcaatctgcc agtcagccac tttctgtgct gccgccattt cgcgcgcttt
    19741 accttttacc gcctgacgaa ctgccagcgc gacgcgcgcg ccggtctggg taacgtcttc
    19801 gcccagcacc agtaccgcat cgtaagattc gatttcgcgc agtgccggcg tataaatgcc
    19861 gccttcacgc agcactttca gcgccagttg cagacgttcc tgctcaccgt gagcgatacc
    19921 ggtgtagaag ttttcttcgc ccaccagctc acgcagcgca aagttgcttt ccacgctggc
    19981 acgcggagaa ccaataccga tcactttctt cgactgacgc agaatatctg ctgcgccctg
    20041 cattgcctgt tcggcgttga gggtaatgaa atcatcgcca cgacgctgta ctggctgacg
    20101 cggacgatct ttcaggttca cgtaaccgta accgaaacga ccacggtcgc agaggaagta
    20161 gtggtttact gtaccgttgt aacggttttc gattcgacgc agttcgccat aacgctcgcc
    20221 ggggctgatg ttacagccga tggaacattg ctggcagatg ctcggcgcaa actgcatatc
    20281 ccatttacgg ttgtaacgct cggagtgcgt tttgtcggtg aatacgccgg tcgggcaaat
    20341 ttcgaccagg ttaccggaaa attcgctttc cagcgtgccg tcttccgggc gaccgaagta
    20401 gacgttgtcg tgcgcaccgt aaacgcccag gtccgtgccg tcagcgtaat ctttgtagta
    20461 acgcacgcag cggtagcagg cgatgcagcg gttcatttcg tgagagatga acggccccaa
    20521 atcctgatta cggtgggtac gtttggtgaa acggtagcga cggaagctgt gtccggtcat
    20581 cacggtcata tcctgaagat gacagttacc gccttcttca cataccggac aatcgtgcgg
    20641 gtggttggtc attaaccact cgaccacact ttcacggaac tgtttcgctt cttcgtcgtc
    20701 aatggaaata aaggtgccat cggaagccgg tgtcatacag gacatcacca ggcgaccacg
    20761 cgtgtcttcc gcgttttggt attgcttcac cgcacactgg cggcaagcac cgacgcttcc
    20821 cagcgccgga tgccagcaaa agtaaggaat atcaaggccc agagacagac aagcttccag
    20881 caggttgtcc gctccgttaa cctcgtattc tttgccgtct acatgaattg tagccattag
    20941 catgcttcca gttttctcag tcagagactg agcgttaatc gaaattcggt taccagcgct
    21001 ctttcagcag gttcggctga atcccattaa tcaaatgggt attgctgaac ggctgtttaa
    21061 ttcccgcttc aaattcttcg cggaaatatt tgatggcgct ctgtaacggc tccactgcac
    21121 caggtgcgtg ggcacagaaa gttttacccg gacctaagaa tcgacacagt tgctcaagtg
    21181 tttcgatatc gcccggctga ccttcgccac gctccagcgc gcgcagaatt ttcacgctcc
    21241 acggcagacc gtcgcggcac ggcgtacacc agccgcagga ctcacgggcg aaaaactctt
    21301 ccaggttacg caccagcgac accatgttga tctcgtggtc aacagccatc gccagcgccg
    21361 tacccagacg gcttcccgct ttaccgatac tttcgaattc catcggcagg tcaaggtgcg
    21421 cttcggtcag gaagtcggtg cccgcgccgc ctggctgcca ggctttgaat ttcagaccgt
    21481 cacgcatacc accggcgtaa tcttcgagga tctcgcgtgc ggtggtgcca aacggcagtt
    21541 cccacagacc cggatttttc acccgaccgg agaagcccat cagcttggtg ccagcatctt
    21601 tacttttcga gatgttctga taccactcca cgccgttagc aaggatcgcc ggaacgttac
    21661 acagggtttc gacgttattg acacaggtcg gtttgcccca tgcaccggag gttgccggga
    21721 atggaggctt cgagcgtggg ttagcgcgac gcccttccag ggagttgatt aacgctgttt
    21781 cttcaccgca gatgtagcgc cctgccccgg tatggacgaa caattcgaag tcgaaacctg
    21841 ttcccataat gtttttgcca agcagacccg cttcggtggc ttcggcaatg gcacggcgca
    21901 gattcactgc cgcttcaata tattcgccgc gcaggaagat gtagccacgg taagctttca
    21961 gcgcaaacgc ggagataagc ataccttcca ccagcaggtg cggcagttgc tccatcagca
    22021 ggcggtcttt ataggtgccc ggctccattt catcagcgtt acacagcagg taacggatgt
    22081 tcatggattc gtctttcggc atcaggctcc acttcaggcc cgtcgagaag cctgcgccgc
    22141 cgcgcccttt caggccagca tctttcacct ggttgacgat ctcgtcaggc gacagcccgg
    22201 tcagcgcttt acgtgcccct tcgtaaccgt ttttgctgcg gtattcgtcc agccacactg
    22261 gctgtttgtc atcgcgcaga cgccaggtca gcggatgcgt ttcgggagta cggataatgt
    22321 ttttcattta taccgctcca gcagttcagg gatcgcttcc ggggtcagat gtgcgtgagt
    22381 atcctcatcg atcatcatgt ttggcccttt atcacagttc cccaggcagc aagttggcag
    22441 cagcgtaaag cgaccgtcaa atgtcgtttg tcctggtttg atgttcagct ttttctcgag
    22501 cgccgcctgg atgccctggt aaccgttgat atgacagacc acgctgtcgc aataacggat
    22561 cacatggcgg ccaaccggct ggcggaagat ctggctataa aacgttgcca caccttcgac
    22621 gtcgctcgcc ggaataccca gcacatcggc gatcgcgtgg atcgcgccat ccggcaccca
    22681 gccacgctgc ttctgaacga ttttcagcgc ttcaatggac gccgcacgcg ggtcttcgta
    22741 gtggtgcatc tcgtgctcaa tcgcttcacg ctctgccgca ctcagctcaa aagcctcggt
    22801 ttgtggttgt tgattctcgt gcataattag cggtccacat ctgacataac aaaatcgata
    22861 ctgcccagat aaacaatcag gtcagacacc aggctgccgc ggatcgccgc cggaatttgc
    22921 tgcaaatgcg cataactcgg cgtacggata cgggtgcgat aactcatggt gctaccgtcg
    22981 ctggtcaggt agtaactgtt gatccccttg gtcgcctcaa tcatctggaa agattcattg
    23041 gctggcatca ccggacccca cgacacttgc aggaagtggg tgatcagggt ttcgatatgt
    23101 tgcaacgtgc gctctttcgg cggcggcgtg gtcagcgggt gatccgcttt gaacgggcct
    23161 tccggcatgt tgttgaggca ctgctcaaga atgcgcagac tctggcgcag ctcttccact
    23221 ttcagcatta cgcgggtgta gcagtcagaa acaccaccac ccaccgggat ttcaaagtcg
    23281 aagttttcat agccagaata cggacgcgcc ttacgcacgt cgaagtcgat cccggtagca
    23341 cgcaggcccg cgccagtggt gccccactcc agtgcctctt tcgcgccata ggcggcaacg
    23401 ccctgggaac gacctttcag aatggtattt tgcagcgccg ctttctcgta agacgccaga
    23461 cgtttcggca tccagtcgag gaactcacgc agcaggcgat cccagccgcg cggcaggtcg
    23521 tgcgctacgc cgccaatacg gaaccacgcc gggtgcatac gaaaacccgt gattgcttcc
    23581 accagatcgt aaattttctg acgatcggta aaggcgaaga acactggcgt catcgcgccg
    23641 acgtcctgaa taaaggtgga gatatacagc agatggctgt tgatgcggaa cagctcagaa
    23701 agcattacgc gaatgacgtt aacgcgatcc ggcacggtga tcccggccag tttctctacc
    23761 gccagcacgt aaggcatttc gttaacgcag ccgccgaggt attcgatacg gtcggtatac
    23821 ggaatgtagc tatgccagga ctggcgttcg cccattttct ccgcaccacg gtggtggtag
    23881 ccgatgtctg gtacgcagtc gacaatctct tcgccatcga gctgcaaaac gatacggaaa
    23941 gcaccgtgcg cagacgggtg gttcggaccg aggttgagga acatgaagtc ctcgttttcg
    24001 gtgccgcgct tcatccccca ctcttccggt ttgaaggtca gggcttccat ttccagatcc
    24061 tgtttggctt tggtcagctc aaacggcgag aattcggtag cgcgtgccgg gtaatcttta
    24121 cgcagcggat gacctttcca ggtttgcggc atcatgatgc ggcgcaaatt aggatgaccg
    24181 tcgaaagtaa tgccaaacag atcccaggtc tcacgctcat accagttagc gttcgggaac
    24241 agtttggtga aggtcggtac gtgcaggtcg ttttctgcca gcgccacctt cagcatgatg
    24301 tcgcggttac gatcgataga aatcagatgg tagaaaacgg aaaaatccgc ggcaggtaac
    24361 ccttcgcggt gtgtgcgcag acgttcgtcc atgccgtgta agtcaaacag catgacgtaa
    24421 ggtttcggca gtttctttaa gaaatcgcca acttccagta attgttcacg cttgatccac
    24481 acaacgggaa ccccggtgcg agtcgcctga acagtaaagg catccggccc aaaacggttg
    24541 cgcagttcgc caatcaccgg atcatcaaga tgatcgcggg tctgccaggc gggttcttgc
    24601 gcggttaagt cggtcatatt gttcaccatt gcaaatggtc cgtggtgact gtcggcgcag
    24661 taagcttcgc gaaatagatt taggaataag cgaagaaaaa tccctttgcc gacaggcgca
    24721 aattaaatct cgtcaggtgt acgcaggttg gttacggcaa tgcgttcacc gcgcttgcgt
    24781 tcgcgctctg attgcatatt ggcgcgataa acgccctgat cgccaaccac ccaggagagc
    24841 ggacgacgtt ctttgccgat agattcctgc aacagcatca gtgcctgcat gtacgcttca
    24901 ggacgcggcg ggcagcccgg gatatacaca tcaaccggga tgaatttatc aacgccctgc
    24961 acaacggaat aaatatcgta cataccgcca gagttggcac aggcacccat tgagataacc
    25021 catttgggtt ccagcatctg gtcatacaga cgctgaataa ccggtgccat tttggtaaag
    25081 caggttcctg ccaccaccat caggtcagcc tgacgcggcg aagcacgcaa tacttctgcg
    25141 ccaaaacgcg ccacgtcatg caccgcggta aacgaagtca ccatctcaac gtaacagcag
    25201 gaaagaccga agttatacgg ccaaattgag tttttacgac cccagttaac catgtcattg
    25261 agcttgccca taaacacgtt tttgttaact tcttgctcca gagggtcggt tacgatctcc
    25321 tgcttttgca gggggtaacg gtcgttctca ccgttgggat ctatgcgggt gagcgtataa
    25381 tccatcttaa tgcctcgcgg ttagcgttga cgattagcga tactgttcgt ttccgggttc
    25441 atacgctcgc ggcgtgaacg cgcgggcgtc cagtccagcg cgccaatacg caccagataa
    25501 accagacctg ccagtaacac aaaaataaaa attgcagctt ccacaaagcc tacccagccg
    25561 ctttcgcgga tagaggttga ccatgcgaac agatacagcg cttcaacgtc gaagataacg
    25621 aagaacatgg ccaccagata aaacttggcg gacaggcgta agcgggcgga gccgaccgag
    25681 tcgataccgg attcaaacgg aacgtttttc gaccttgcgc gtgcgcgacc gcctaaaaac
    25741 caaccgccta ccagcatcag gcaacacagg ccaatggcaa cgataagaaa gatagcgaat
    25801 gcccagtgat gagcgatgac ttcagtggat gttgacatac tcattgctta ctcatcaaaa
    25861 gtagcgccag attcactgct cttcacggca gatggacgcc acatcgattc atggggagga
    25921 ataaaaaaaa ccttacaatc actgtagaaa ttcttttata cagctaattg atgtggtctt
    25981 ttactccttt ctataacctt ttgtcaactt taacaaaagt ttcttcacat tagtttacat
    26041 aatatcaaca ccattagcat ttaatgccct ttcaccccag atccttgacg actccaggat
    26101 aattagatgt tgttgaatcg tgtccgttgt gaagcaatgg aaaaaatacg ggtctatttt
    26161 gacaggaatt tgtgtcgatt cctcccccca aaagagagta ttttcttgat ctgtgacacg
    26221 cttttgtcat tccataacaa aaacgcagca acaaatttac gtatttttta acatcattgt
    26281 agcaggtgat ttttttcagg cgattatttg tgcgttcggg acgtgaatct ctggtgattg
    26341 aaaaacgaac agttttgtac gttctgcacg atacgaggaa ggctttttac taaaaaaaag
    26401 ccgctggggt ttaaaacacc cccagcggct cgtttttaca ctattgtctc agaaattatc
    26461 tatggtccgt cgattactcg atatcccttt caatcaacaa tgagtcatcg ccttccggag
    26521 cagacattgg gctgtattgc cacggattat ggtagctctc catcgcctga tagatcacct
    26581 gggccagctc attattactg gacgggtcat agcacagcaa atattcggta tcgggtagcg
    26641 gcggtaagcc atctactcca ctcagcacac gcaggtccgg actcatcatc tcgaccggcc
    26701 ttgccgtcac gccaagaccg gcttttactg ccgcacgaac tgccggaagc gtcgaggcga
    26761 cataagccag tcgccaggga atatctgctt tattaagcgt cgccagcacc atatcgcgaa
    26821 acgggctagg atcatccagc aatacaagag ggatcggctc gcctttttgc aatacgtatt
    26881 ccgctgcgca gtaccagtgt gttggcgagg tacgcaggtt caatgcctta aacgttgaag
    26941 gacgatgggt ggttaccatc aaatccactt cctgggattc cagcatttct gccatatagg
    27001 cattacgttt aacgcggaca tccagcgcta gtttcggata aaccgaactc acgcgattta
    27061 acaggaaagg taagatcgta tcggcagatt catctgaagc accgatagtt aacacgccct
    27121 gaagattact gaacattaat gatgagcagg cctcatcatt aaaacgcaga atttttctgg
    27181 cgtaaccaag aagttgaatg ccatgttcag ttaacagttt gttgcgaccg tgacgagcga
    27241 acagctcttt cccaacgagt tgttccagac gctgcatttg ctgacttacg gcggactgag
    27301 tacgacacac agcggcagct gcggcagcaa aagtgttcag atcggcaaca gcaacaaatg
    27361 ttctcagcag atcgaggtcg aggttaatta tcggacgatt tgcacttatc atatattatc
    27421 acttactggc ggctcatact gagctggtta atgctgtgca cacacaaaca agcaattcca
    27481 tttgtaatgt gcctccctgg cagtttcatc cggaacccgg acgaaagtaa aaatgcatat
    27541 gagttgcact aaaaaagcga ctcacattgt tccgttataa tgcctgaagt agatcacaga
    27601 atatatcttc aggaatcgca tatctattaa gttactcact cttttgtatt tatgacatgc
    27661 gcgtgtttgt ataaatgtaa atgtgagtcc ttgttccact ctcgtgcagc atcgctggtc
    27721 atacgcgaac acgtacaaac agcaatggtg aggcggcatc aagagcaggg atccgtcatt
    27781 tatgcgagca ttttacccca aaaacctttt atttataagg gtcattgcga attatctgat
    27841 gcaaagttat gttatgttag gcaaagtaat cttctattat taataagcac aacaaaacct
    27901 ttttgaatat taaataataa ttaattagca tcatcctcat tcattaatcc cgcttaacaa
    27961 tagtttcaca ccttgcaccc attacgacac ctcggaaatc atcacgctgt taaactctcc
    28021 gtaatgcgga aatatcatca tcacgattca ttagtgaatt cttaaacgag gcattttcaa
    28081 acaattatct tacagataaa aaaaccacac tcacaattaa gaatcagaac aattcactac
    28141 ataacattaa ttattaagca tatacacctc attattttgc cattattaag ttattaacag
    28201 cacaatcaag ccttccactc tggcaaaatc ttattctgca gaccttcaaa acgccgtcct
    28261 gggggagtac attgttctaa gctgacttcc acggcaggga gtggcgataa cagcaaaaaa
    28321 ggtcaagatt catgtccccc attgaaaaat ccagcaaatt agagaatgtc tgttacgaca
    28381 tccgtggtcc ggtgctgaaa gaagcaaaac gcctggaaga agaaggtaac aaggtactga
    28441 aactgaacat cggcaatcct gccccgttcg gttttgacgc gccagatgaa atcctcgttg
    28501 acgtgattcg caacctgcct accgctcaag ggtattgcga ttccaaaggg ctttactccg
    28561 cacgtaaagc catcatgcag cactaccagg ctcgtggcat gcgtgatgtt accgtggaag
    28621 atatttacat cggcaatggt gtatcggagc ttatcgttca ggcaatgcag gcattgctga
    28681 acagcgggga cgaaatgttg gttcctgcac ctgattaccc actatggacc gcggcggttt
    28741 cgctctccag cggtaaagcg gtgcattatc tttgcgatga atcctctgac tggttcccgg
    28801 accttgatga tattcgcgct aaaattacgc ctcgtacgcg tgggatcgtt attatcaacc
    28861 caaataaccc aaccggcgcg gtatattcca aagagctttt aatggagata gtggagattg
    28921 cacgtcagca taatctcatt atcttcgccg atgaaattta tgacaaaatt ctctacgacg
    28981 acgctgagca tcactcaatt gcgccgctgg cacctgatct gctgaccatt acctttaacg
    29041 gactgtcgaa aacgtaccgc gttgctggct tccgtcaggg gtggatggtg ttgaacgggc
    29101 cgaaaaaaca cgccaaaggt tacatcgaag gtctggaaat gctggcttca atgcgcctgt
    29161 gtgctaacgt tcctgcgcaa cacgccattc agaccgcgct gggcggttat cagagcatca
    29221 gtgaatttat tacccctggc ggtcgtcttt atgagcagcg taaccgcgca tgggaattga
    29281 tcaatgatat tccgggcgtt tcctgcgtga aacctcgtgg tgcgctgtac atgttcccga
    29341 aaatcgacgc caaacgcttt aatattcacg acgatcagaa aatggtgctg gatttcctgt
    29401 tgcaggaaaa agttctgttg gtgcaaggga cggcattcaa ctggccgtgg ccggatcact
    29461 tccgcattgt cacgctaccg cgtgtcgatg atatcgagct gtctttgagt aagttcgcgc
    29521 gtttcctttc tggttatcat cagctgtaat cttaatttca ctgccggaga ttgcatccgg
    29581 cagtgttatc ccgccacaat gacctgatga tgtcatcata cgtaaggtca ctatgaaaca
    29641 gagccatttt tttgcccatc tctcccgcct gaaactcatt aaccgctggc cgctaatgcg
    29701 caacgtgcgg acggaaaatg tgtccgaaca cagtttgcag gtagcgatgg tcgcccatgc
    29761 gctggctgct atcaaaaatc ggaaatttgg cggtaatgtc aacgccgaac gtatcgcttt
    29821 actggcgatg taccacgatg cctcagaagt gcttaccggc gatctcccta ccccggtgaa
    29881 atacttcaat tcgcaaatcg ctcaggagta caaggcgatt gaaaaaatcg ctcagcaaaa
    29941 actggtcgat atggttccgg aagagctgcg ggatatcttt gcgccgttaa ttgacgagca
    30001 tgcatatagc gatgaagaaa aatcgctggt gaaacaggca gatgcactct gtgcatatct
    30061 gaaatgtctg gaagaactcg cggcgggaaa taatgaattc ttgctggcaa aaacgcgact
    30121 ggaagcgacg cttgaagcgc gccgcagcca ggagatggac tacttcatgg aagtctttgt
    30181 tcccagcttc catctttcgc tcgatgagat tagccaggat tcaccgctgt aagcagccgg
    30241 agtctgcgtc gcatcgggca ataagcgccg gatgcgacat cagcctcttg tcaaaacgga
    30301 aacagcatcg ggatcatcac cacacaaacc gccatcacga taatggtgaa cggtaccccc
    30361 aacttcacaa agtcactgaa gctgtaattt cccgggccta aaaccagtgt gttaaccggt
    30421 gaagaaaccg gtgtcataaa ggcggcggat gctgccatcg ccacgaccat cgcaaatgga
    30481 taaggcgaca cacccatcgt tttggcagca gccagcgcaa tcggagccat caacaccgcc
    30541 gtcgcggtat tagaaataaa tagcccaata accgccgaca agacaaacaa acaccccagc
    30601 atcatatgtg gcccataacc gccgccaatg tccatcagcc ctttcaccgc cagcgcgaca
    30661 cctcccgttt tctgtaatgc cacagcaaat ggcatcatcc caacgatcaa aataatgctc
    30721 ggccagtgaa tggatttata ggcgctttca gcatctatac agcggaattt ccccatcagc
    30781 aggcaggcga tgatagcggc aacaggatta ggaatttcat cggtcagcat taacgccacc
    30841 atcaacacca ggcaaaaaat ggcatgaggt gcctgactgt gtgctggcga tgcttcactc
    30901 acttcttctg gtaagttcag cgccacaaag tcacgcccct gttttgccag cataccgatc
    30961 agtttccagt tacccacaac caggatgata tcgcccagca gcagaggctc atccgccagc
    31021 gaaccttcca tcgcctcgcc attgcgcttt agccccacca cattcagtcc gtagcgggta
    31081 cgaaaaccaa tttcgcgcac cgatttacca ataagttcag actcaggaat taatgaaatc
    31141 tctgccatgc ccacatcaag ggcctggtca gaaaaatact cgccgcgcag taccatcggc
    31201 tccagcaatt gctcactaca aaattgccgg agatcgacat cagccgcaga catatcaata
    31261 agcaaaacgt cacgcgcgcg aaattcagaa accccattaa cgttcacgat aacgcgacga
    31321 aaacgccgcc agcgttcaac accgatgacg ttagcgccat aacgctcacg taatttgaga
    31381 tcgtccagcc gttgaccaat cattggcgaa ccggggcgaa tagccagacg acgcgcacgc
    31441 ccggtcagtc gatattcacg gataagatcg cgaaaggttc gacgcgtcca gccttcgcgc
    31501 tgcggggtct gggtatcccc tttcagcatg aaacgcatca ctaacatata caaaataccc
    31561 agcaccagca cgaccaggcc aataggtgtt acgctaaaga aactgaagcc gtgatagcct
    31621 tcacgcagca attcactgtt gactaccagg ttcggcggcg tcgccaccag cgtcatcatg
    31681 ccgctaatca gcccggcaaa actcaacggc atcatcaggc gcgacggcga agtttgcata
    31741 cgcatggcaa cgcttaacac cacgggaata aagatagcga caacgccggt tgaactcata
    31801 aacgcgccaa gccccgcgac ggtcagcatc aacaaaacca acattttgat ttcactgttg
    31861 cccgccactt tgaccagcca tgtccccatt acagtggcaa caccggtacg gaccaaacca
    31921 tcgccaataa tgaacaaggc ggcaatcagg acaacgttag gatcggaaaa gccggaaaat
    31981 acttcgggga ccgtcagcgt tccgcttaat gcaaacgcga caataacaaa caaagcgacc
    32041 gcatccatac gcactctgcc cgtcgcaaac aagacgatgg caacggccag taatgaaaga
    32101 acccaaatca attcaccgtt cacaacttat ccttgttaat tgagggggat gacttgattc
    32161 tgccataaaa aagccccgac gagacggggc taaatcatga tcaggtgttt cactgaataa
    32221 taacatcgcc atttggctgt ttggtcacag taatttgctc cagactgtgg aggactaaat
    32281 cgacctcatt caggcgcggg gtatctgccg gagcgttaac cgcaatgaca tgacaccccg
    32341 ccgccaggcc agaaagcacg ccagcgggag catcttccac caccacacac tcctgcggcg
    32401 caagccccag cagctgcgcg cctaacagat acgcatcagg ttctggtttt ccgcgcttca
    32461 ctcgctcagc ggttacaaac acctctggtg cgggaagccc agctacttta tggcgcgctc
    32521 gcgctaccgg catggagcca gaagtcacaa tggcccacgg aatacctgct ttattcaaat
    32581 gattgagtaa ggcgattgcc cccggaagcg cggtaatacc ctcggtttcc gtggcctcga
    32641 tttgctccag acgcgtaaac tcggcggcaa tatcagcctc ggatttgccc gccataaaat
    32701 ggcgcagaga ggtgatcgcc tgtttaccgt gaatgaaagc cagcacctct tccggcgcta
    32761 acccatgacg tctggcccag ttgctccacg cccgctctac cgcaggcagg gaatccacca
    32821 gcgttccatc aagatcaaac agaaaacctt tgcaccgcac gcgggcctcc tcaggcgttg
    32881 ataatttgat taatttcgtt ggcgctcaaa tggtactgac gcgggcaggc atgccacaca
    32941 ttaagcatgc gctgatattt ttcccacatt ggcgtctggg cgttaaaacc gtgagttccg
    33001 gcgtcaaaat gggtatagcg cccttccaca ttaaccataa agcggacata accgaggtaa
    33061 cgtgcttcag tggcggcgtc aaagccgagg aaggtgacac gacgttcatc gatggattgc
    33121 tggtcctgca aattagacca ggaaacatgc aacgcatgat acatctccat aatgtcgatg
    33181 atagtgcggc aggtttcttc tttcagctcg ccaaactcgc gatccagttc acgcatctgt
    33241 aatccataac cacgctcaat gattgtttgc aggcgacggt aacgttcagc atttgccgga
    33301 tcgagcatag tcatcatctt gtactggtta gacaaaataa gacgttgcgc gttggtcatt
    33361 tccattgttg actcctgtat cactctacta cggtgaaaaa aaagaaggct gagtatgcct
    33421 tcttttatat gcgtaatcag gggtcaatta caaatcatca aggaaagttt tatccagttg
    33481 tttgaaggcg cgcttaagcg tgtcagctaa tgcctggtaa tcaggcttgc cttcaacggg
    33541 tgccaacacc tgtccagact cctgcaattt accgcgaact tcataaaacc agttgaggat
    33601 agcagggggt aatggcgtta cagaacgctt gcccagccac cacaatccct gcatgggtaa
    33661 acttaaggcg aacagcgcag tggcaactgc cggcccaagc tgaccgccca gggcaatctg
    33721 ccagcagaga gtaaatacgg cgatcggcgg cataaaacgg atcgcataac gcgtcatctt
    33781 gataacgcga ttttcgacaa agactggggc aaggcgtttt tccagcggcc acgtctttga
    33841 gtaatgctgt ccccggcgaa acaagctaaa aaaattaaca gaacgattat ccggcgttga
    33901 catgcttcac ctcaacttca catataaaga ttcaaaaatt tgtgcaaatt cacaactcag
    33961 cgggacaacg ttcaaaacat tttgtcttcc atacccacta tcaggtatcc tttagcagcc
    34021 tgaaggccta agtagtacat attcattgag tcgtcaaatt catatacatt atgccattgg
    34081 ctgaaaatta cgcaaaatgg catagactca agatatttct tccatcatgc aaaaaaaatt
    34141 tgcagtgcat gatgttaatc ataaatgtcg gtgtcatcat gcgctacgct ctatggctcc
    34201 ctgacgtttt tttagccacg tatcaattat aggtacttcc atgtcgagta agttagtact
    34261 ggttctgaac tgcggtagtt cttcactgaa atttgccatc atcgatgcag taaatggtga
    34321 agagtacctt tctggtttag ccgaatgttt ccacctgccc gaagcacgta tcaaatggaa
    34381 aatggacggc aataaacagg aagcggcttt aggtgcaggc gccgctcaca gcgaagcgct
    34441 caactttatc gttaatacta ttctggcaca aaaaccagaa ctgtctgcgc agctgactgc
    34501 tatcggtcac cgtatcgtac acggcggcga aaagtatacc agctccgtag tgatcgatga
    34561 gtctgttatt cagggtatca aagatgcagc ttcttttgca ccgctgcaca acccggctca
    34621 cctgatcggt atcgaagaag ctctgaaatc tttcccacag ctgaaagaca aaaacgttgc
    34681 tgtatttgac accgcgttcc accagactat gccggaagag tcttacctct acgccctgcc
    34741 gtacaacctg tacaaagagc acggcatccg tcgttacggc gcgcacggca ccagccactt
    34801 ctatgtaacc caggaagcgg caaaaatgct gaacaaaccg gtagaagaac tgaacatcat
    34861 cacctgccac ctgggcaacg gtggttccgt ttctgctatc cgcaacggta aatgcgttga
    34921 cacctctatg ggcctgaccc cgctggaagg tctggtcatg ggtacccgtt ctggtgatat
    34981 cgatccggcg atcatcttcc acctgcacga caccctgggc atgagcgttg acgcaatcaa
    35041 caaactgctg accaaagagt ctggcctgct gggtctgacc gaagtgacca gcgactgccg
    35101 ctatgttgaa gacaactacg cgacgaaaga agacgcgaag cgcgcaatgg acgtttactg
    35161 ccaccgcctg gcgaaataca tcggtgccta cactgcgctg atggatggtc gtctggacgc
    35221 tgttgtattc accggtggta tcggtgaaaa tgccgcgatg gttcgtgaac tgtctctggg
    35281 caaactgggc gtgctgggct ttgaagttga tcatgaacgc aacctggctg cacgtttcgg
    35341 caaatctggt ttcatcaaca aagaaggtac ccgtcctgcg gtggttatcc caaccaacga
    35401 agaactggtt atcgcgcaag acgcgagccg cctgactgcc tgatttcaca ccgccagcta
    35461 agctggcggt gctgttttgt aacccgccaa atcggcggta acgaaagagg ataaaccgtg
    35521 tcccgtatta ttatgctgat ccctaccgga accagcgtcg gtctgaccag cgtcagcctt
    35581 ggcgtgatcc gtgcaatgga acgcaaaggc gttcgtctga gcgttttcaa acctatcgct
    35641 cagccgcgta ccggtggcga tgcgcccgat cagactacga ctatcgtgcg tgcgaactct
    35701 tccaccacga cggccgctga accgctgaaa atgagctacg ttgaaggtct gctttccagc
    35761 aatcagaaag atgtgctgat ggaagagatc gtcgcgaact accacgctaa caccaaagac
    35821 gctgaagtcg ttctggttga aggtctggtc ccgacacgta aacaccagtt tgcccagtct
    35881 ctgaactacg aaatcgctaa aacgctgaat gcggaaatcg tcttcgttat gtctcagggc
    35941 actgatactc cggaacagtt gaaagagcgt atcgaactga ctcgcaacag cttcggcggt
    36001 gcaaaaaaca ccaatattac cggcgttatc gttaacaaac tgaacgctcc ggttgatgag
    36061 cagggtcgta cccgtccgga tctgtccgag atttttgacg actccaccaa agcaaaagtg
    36121 aacaacgttg atccggcgaa gctgcaagaa tccagcccgc tgccggttct cggcgctgtg
    36181 ccgtggagct ttgacctgat cgcgactcgt gcgatcgata tggctcgcca cctgaatgcg
    36241 accatcatca acgaaggtga catcaatact cgccgcgtta aatccgtcac tttctgcgca
    36301 cgcagcattc cacacatgct ggagcacttc cgtgctggtt ctctgctggt gacttccgca
    36361 gaccgtcctg acgtgctggt tgccgcgtgc ctggctgcca tgaacggcgt agaaatcggt
    36421 gccctgctgc tgactggcgg ctacgaaatg gacgcgcgca tttctaaact gtgcgaacgt
    36481 gctttcgcta ccggcctgcc ggtatttatg gtgaacacca acacctggca gacttctctg
    36541 agcctgcaga gcttcaacct ggaagttccg gttgacgatc acgagcgtat cgagaaagtt
    36601 caggaatacg ttgctaacta catcaacgct gactggatcg attctctgac tgccacttct
    36661 gagcgcagcc gccgtctgtc tccgccagcg ttccgttatc agctgactga acttgcgcgc
    36721 aaagcgggca aacgtatcgt tctgccggaa ggtgacgaac cgcgtaccgt taaagcagcc
    36781 gctatctgtg ctgaacgtgg tatcgcaact tgcgtactgc tgggtaatcc ggcagagatc
    36841 aaccgtgttg cagcgtctca gggtgtagaa ctgggtgcag gcattgaaat cgttgatcca
    36901 gaagtggttc gcgaaagcta tgttggtcgt ctggtcgaac tgcgtaagaa caaaggcatg
    36961 accgaaaccg ttgcccgcga acagctggaa gacaacgtgg ttctcggtac gctgatgctg
    37021 gaacaagatg aagttgatgg tctggtttcc ggtgctgttc acaccaccgc aaacaccatc
    37081 cgtccgccgc tgcagctgat caaaactgca ccgggcagct ccctggtatc ttccgtgttc
    37141 ttcatgctgt tgccggaaca ggtttacgtt tacggtgact gtgcgatcaa cccggatccg
    37201 actgcagaac agctggcaga aatcgcgatt cagtccgctg attccgctgc ggccttcggt
    37261 atcgaaccgc gcgttgctat gctctcctac tccaccggta cttctggtgc tggtagcgac
    37321 gtagaaaaag ttcgcgaagc aactcgtctg gcgcaggaaa aacgtcctga cctgatgatc
    37381 gacggtccgc tgcagtacga cgctgcggta atggctgacg ttgcgaaatc caaagcgccg
    37441 aactctccgg ttgcaggtcg cgctaccgtg ttcatcttcc cggatctgaa caccggtaac
    37501 accacctaca aagcggtaca gcgttctgcc gatctgatct ccatcgggcc gatgctgcag
    37561 ggtatgcgca agccggttaa cgatctgtcc cgtggcgcac tggttgacga tatcgtctac
    37621 accatcgcgc tgactgcgat tcagtctgca cagcagcagt aatctcgtca tcatccgcag
    37681 ctttgcgctg cggatatcgg aaccggaaat agtcactatt tccggttttt tattctctta
    37741 atttgcatta atcctttctg attatcttgc ttaactgcgc tgcatcaatg aattgcgcca
    37801 tctcactttg catacttacc actttgtttt gtgcaaggga atatttgcgc tatgtccgca
    37861 atcactgaat ctaaaccaac aagaagatgg gcaatgcccg atacgttggt gattatcttt
    37921 tttgttgcca ttttaaccag ccttgccacc tgggtagttc cggtgggcat gtttgacagt
    37981 caggaagtgc agtatcaggt tgatggtcaa acaaaaacac gcaaagtcgt agatccacac
    38041 tcattccgca ttctgactaa cgaagcaggc gaacctgagt atcaccgcgt acaactgttc
    38101 acgacgggcg atgaacgccc gggtctgatg aacttcccgt ttgaagggtt aacctcagga
    38161 tcgaaatacg ggacagccgt tggcatcatc atgtttatgc tggtgattgg cggcgcgttt
    38221 ggcattgtga tgcgtacagg aaccattgat aacggtatcc tggcgcttat tcgccatact
    38281 cgcgggaatg aaattctctt tattcctgcg ctgtttattc tgttttcact tggcggtgcg
    38341 gtatttggta tgggggaaga ggccgtcgcc tttgccatta tcatcgcacc gctaatggtc
    38401 cggctgggct atgacagtat taccaccgtc ctggtgactt atattgccac gcaaatcggt
    38461 tttgccagtt cgtggatgaa cccgttttgt gtggtcgttg ctcaggggat tgccggcgtt
    38521 ccggtgcttt ctggctccgg gttgcgcatc gtggtatggg ttatcgccac tctgattggc
    38581 ctgatcttta ccatggtgta cgcctcacga gtgaaaaaga atcctcttct gtcacgcgtg
    38641 catgagtccg accgcttctt tcgtgaaaag caggcggatg ttgaacaacg tccgtttacc
    38701 tttggtgact ggctggtatt gattgtcctg accgccgtaa tggtctgggt gatttggggc
    38761 gtgatcgtta atgcctggtt tattccagaa attgccagcc agttcttcac catgggtctg
    38821 gtgattggca tcatcggcgt cgttttccgc cttaacggca tgacggttaa taccatggct
    38881 tcatccttta ccgaaggggc gcgaatgatg atcgcccctg ccctgctggt gggtttcgcc
    38941 aaagggattt tgctgctggt cggtaatggt gaagcgggtg atgccagcgt gttaaatacc
    39001 atcctcaaca gcattgccaa tgccattagc ggtctggata acgcggtcgc ggcctggttt
    39061 atgttgctct tccaggcagt atttaatttc ttcgtgacgt ccggttctgg tcaggcggcg
    39121 ttaaccatgc cgttactggc accgcttggc gatctggtcg gtgttaaccg tcaggttacc
    39181 gtgctggcgt tccagtttgg tgatggcttc agccacatca tttacccgac ctcagcttcg
    39241 ttaatggcaa cgctcggtgt ttgccgggtg gacttccgta actggctgaa ggtgggcgcg
    39301 acactgcttg gactgctgtt tattatgtcc agcgtcgtgg tgatcggcgc tcagttgatg
    39361 ggctaccact aaaaatgtta agagccgcaa ttgcggctct tttttcattc tgccgtttca
    39421 gtttctacgg cttcattttt ggcattgcgc ttcatccaca acgccagcgc tttcagcgag
    39481 tctggagtga actcatcgca gcgtgcggtg atttcttccg gcgtcagcca gcaaacttca
    39541 ctgacttcat cttcctgtag ggcgaaggga ccgtgagaga cgcaactgaa caacgcgccc
    39601 cagacacggc aatttttatc ttcgaaatag aactgcccgt gctcggcaaa ggggacaccg
    39661 gcaatgccca actcttcttc cgcttcgcga cgcgcggatt ccagcagttg ctcatcggcc
    39721 tggaccacac cgcctgcggt cgcatctaac atgccgggta aaaagtcttt tgtctcggta
    39781 cgacgctgga ccagaatttt gcccatgcca tcatgcacga cgatgtaagt tgcacgatga
    39841 cgcagacact gtgcccgcat ttgttcccgg ctggcttgtg caatgacttc gttctcttca
    39901 ttgacaatat ccacccattc agtacttgcc aaacgacgct gttccattat caggaaacct
    39961 tctttttctg gcgctcttac ggcgcatttt gagttgtggg taaattacgg attaatcgcg
    40021 acctgcgcaa tgatactttg atcattgagt gcgattacac taagtacgtc attatccagc
    40081 atgccataac tcgccggatt accgccttta ggaatactca ccgagccggg gttgaagtgg
    40141 aaaatctccc cccgctgttc tgccactggc agatgggtat gaccgtacac cagcacatcg
    40201 ttctggttta aagcaggtag gttttccggg ccaaaaagat gaccatgcgt caaaaacaga
    40261 cgttgttttt ccagtaatac ctgttgccac ggcgcggtta tcgggaaatg cagcagcatt
    40321 tgatccactt cgctgtcgca gttgccgcgc acagcgataa ccttatgtgc cacttcatta
    40381 agccgttcag cgactttggc tggcgcgtaa ccctccggta aagcattacg cgggccgtga
    40441 ttcaacacgt cgccaaggat caccagccac tgggcaccgc tttgggcaaa caactccaga
    40501 acacgttccg tcgccggtaa cgacccatga atgtccgatg caaacatcag tttcatcact
    40561 cactcctcgt cgaaaaaacg taagctatga tactggattc tttcgttgct atcagccaga
    40621 ttgcttcgcc gaaagagcaa taaaacgctg gacagacgct cgctgccact ggaatgtcgc
    40681 ataatccacc aggcgttccg gcacctcatc gccatgtagt accaggcggt taatcattag
    40741 cgccagatca gtatcagcaa tgcaccattc accaaataaa ttcggccgcc ccagtgctaa
    40801 caaatgtttt gccatcgcga acagtttctc tgcactggct tttccctcgg ccgttagtgg
    40861 cgcttttttc gcccccgcaa agacaacatc cgtcggacgc tcttcgcgga tgggcatcag
    40921 atcgctgcgc aaccaggcct gaatctgtcg tgcacgcgca cgattttcta aatcaagcgg
    40981 ataaatacgt tcccaggtcg gtggcgcaaa tcgatcttcc agatactccg caatggcaga
    41041 agattcactc aactcaaaat catcgatttg taataacggc acacggcgtg tctgaccgta
    41101 accttgccac gtcggttgca aatgttcacc gctgtcgagg tcgatggtct tgatatgaaa
    41161 cgacaggcct ttttcctgca acgccaccca ggcggataac acataagggg agaaacagtg
    41221 ggcatctgac caaagcgtga tagcgggttt actcattatg tcctcggcaa taaagggggg
    41281 ctggctatca aactatagag cctttttgtc actgtcacgt gacgaatact cacgtaagtc
    41341 gccgcgcttt ctatacttgt ggtgttcaga tgattagaca accggagctg caatgatcga
    41401 tctctatttc gccccgacac caaatggtca caaaattacg ctgtttctcg aagaagcagg
    41461 gctggattat cgcttgataa aggtagacct ggggaaaggc ggtcagtttc gcccggaatt
    41521 tttgcgcatt tcgcctaaca acaaaattcc ggcaattgtt gatcattctc cagccgatgg
    41581 cggcgaaccg ctaagcctct ttgaatctgg tgccattttg ttgtatctgg ctgagaaaac
    41641 ggggctcttt ttgagtcatg aaacgcgtga acgcgccgcc acattacagt ggttattctg
    41701 gcaggtaggc ggactggggc cgatgcttgg gcaaaatcat cattttaatc acgcagcccc
    41761 ccaaaccatt ccttacgcta ttgaacgtta tcaggttgaa actcagcgtc tgtaccatgt
    41821 actgaacaag cggctggaaa actcgccctg gctgggtggc gagaactaca gcattgcgga
    41881 tattgcctgc tggccgtggg ttaatgcctg gactcgccag cgaattgacc tcgcaatgta
    41941 tccggcagtc aagaactggc atgagcggat ccgttcgcgc cctgccaccg ggcaggcact
    42001 gctaaaagca caactcggtg atgagcgttc ggatagttaa cagaaacagg ttctcgtgta
    42061 ttatttcatc ctaagtaaaa caacggagaa cctgcaatgg cacaacctgc cgctattatt
    42121 cgtataaaga accttcgttt acgtacgttt atcggaatta aggaagaaga aattaacaac
    42181 cgtcaggata ttgttatcaa tgtgacgatc cactaccccg ccgataaagc gcgcactagc
    42241 gaagatatca acgatgcgct gaattatcgc accgtaacga aaaacattat tcagcatgta
    42301 gagaataacc gtttctcttt gctggaaaaa ttaactcagg atgtgctcga tatcgcacgt
    42361 gaacatcact gggtgacgta tgctgaagtg gagatcgata aactgcacgc gctgcgctac
    42421 gccgattcgg tatccatgac cttaagctgg cagcgttaac cgccatatcg ggaggctgca
    42481 tgaagatagt gatcaccgga gggacgggat taattggtcg ccatttgatt ccgcgtttgc
    42541 tggatctggg ccatcaaatc acggtagtga cgcgtaaccc gcagaaagcc agttccgttc
    42601 tcggccctcg ggtgacacta tggcaagggc ttgccgatca aagtaacctc aacggctttg
    42661 atgcggtaat caacctggcc ggagagccga ttgctgataa acgctggact cacgagcaaa
    42721 aagagcgtct ctgccaaagc cgctggaata tcacgcaaaa actggtcgat ttgattaatg
    42781 ccagcgacac gccaccgtcg gtactcattt ccggctcggc aacgggctat tatggcgact
    42841 taggtgaagt ggtggttacc gaagaggaac cgccgcataa cgaatttacc cataaactct
    42901 gcgcccgctg ggaagaaatt gcctgtcggg cacaaagtga caaaacgcga gtgtgcctgc
    42961 tgcgtaccgg cgtagtgctg gcaccggatg gcggtattct cggtaaaatg ctgccgccgt
    43021 ttcgtcttgg cctgggcggg ccgattggtt ccggtcggca gtatctggcc tggattcata
    43081 ttgatgatat ggtcaacggc attctctggc tgctggataa cgagctgcgc gggccattta
    43141 atatggtttc gccctacccg gtacgcaatg aacaatttgc ccatgcgctc ggtcatgcgc
    43201 tgcatcgccc ggctattttg cgcgtccccg caaccgccat tcggctgtta atgggcgaat
    43261 cttcagtact ggtattaggt ggacaacgcg cgctgcctaa acggctggaa gaagcgggat
    43321 ttgcgtttcg ctggtacgat ttagaagagg cgctggcgga tgttgttcgc tgatgtggtt
    43381 tactgcaaac atccgccagt taactcccgg tgttacagga ttagtggctt tgcgcgataa
    43441 gatcgtctgg tgaaagtcgg gtcaccatca taactaactc tctgtctaaa cctctctcca
    43501 gcatctcctg agcaatacgc agggcttctt cgcgtttgcc cagaattaat ccatcattcc
    43561 gcccttcttc acgtaatctg tcagcaatag tcatcagttt ctccttttct tgtggtgcgc
    43621 gttccgctat ctcaccaata aatgcacgga aacgctgggc atctcccgtt tgtaatacgt
    43681 aattaaacag ggcttttagc tgcctgtcat tagtgtttcc tgtaactagc agcgaaacaa
    43741 tttggtcgac taatcccaac agatcgcgct gacgaatatg tttctgaatt aactccaaca
    43801 gcgccatttt gcgatgttgc ataatctcgt catccggcac cacggtaata tccaccagcg
    43861 gaaaagctga tgaatatatt ttgcgggcta tagcaggctc ggcaaattca tcaagccagc
    43921 agagtgaata aggataagga cttctgcaac catgataaaa cagcatcggg atcaccaatg
    43981 gaagctcttt atagcctgca tcaagatggt tttgcatcgc cgcaatggaa tagcgcatca
    44041 tgcgaaaagc cattaattct tccggcttac tttggtgctc tatcaccaca taaatataac
    44101 ccgctccctc ctgcgttttc acagaccaca agaggtcgga ataatattgc cgcaggtctt
    44161 catcaataaa actgtttggt tccagtttaa gcgtcgttaa atcacacagt ttgcgcagcg
    44221 gcgcggggag atgaatatca ataaaatccc gggcggtgtc tggatggcgt aaaaaagatt
    44281 taaataccgc atcatgcggc gtggaagttg tcgatattgt catggcgttc cgtcacccct
    44341 caaatttaag atgacgcgac aataaccacg attaacaccc aatgcagcag gcagttttta
    44401 tctttggaca gcgaattcag aagtattttt agcgggctgc aacttttaca tttcagcgga
    44461 agcggactcg caaatttccc ttgaaaatac tccggcagcg ttagctaccg gagggggaat
    44521 tatttcagcg atcccttaag gaactgttgc agacgagggc tttgcgggtt gccaaataac
    44581 tgctccggcg cgccttcttc ctctattttc ccctgatgga gaaaaatgac gtgagtagaa
    44641 acatggcgag caaagcccat ttcgtgggtc accaccacca tggttttccc ctcttctgcc
    44701 agttgctgca taatacgcaa cacttcgcct accagttcag gatcgagcgc cgaggtaggt
    44761 tcatcaaaca gtaaaacttc cggttccatt gccagcgccc ttgcgatgga aacacgctgt
    44821 tgctgaccgc cggaaagatg caccggatat ttcccctgcg cacgttcgtc tatcccgact
    44881 ttcgccagat acttcaccgc ccgctcgcgc gcttcctgct tgctcaggcc caacacctga
    44941 atcggcgctt ccatgacgtt ttccagcacc gtcatatggc tccagagatt gaagtgctgg
    45001 aataccatcg tcaggcgtgt gcgcagtaag cgcagctgat ttttatcggc gactttgagt
    45061 tgaccatctt tgtcgcgcac cagattgatc gtctggccat tgaccacgat cgatccttcg
    45121 ctcggttttt cgaggaagtt aatgcagcgc agaaaggtac ttttccccga tcccgacgat
    45181 ccgatgatgc taattacatc tccggcattc gcttgcagtg aaaccccttt cagcacttcg
    45241 tgttcgccgt agcgtttgtg caaatcgata acgtttaatt tattctcgga catcgtattc
    45301 tcagtgcgtt gaagaaggtt tcacatgctg caaccagcgt ttttccgctc tgcgaaagag
    45361 gctgatcagg acataagaga tgattaaata gagcaccgcg gcaatgccga aggcggtaaa
    45421 aggttgatac gtggcggcgt taatatcgcg ggctattttc agcagatccg gcacagtggc
    45481 cgtaaacgcc agcgcagtag agtgcagcat caggatcact tcgttgctgt atgccggtaa
    45541 cgcaatacgc agcgcagaag gcaaaataat gcagcgatac attttaaagg tcgagaagcc
    45601 ataggctctg gcggcttcga tttccccatg cggcactgaa cggatcgctc cggcaaaaat
    45661 ctcggtggtg taagcacagg tgttaagcgt cagcgccagc acggtacagt tcaggccact
    45721 gcgaaaaaag gcgttaagga actcggttcc cttaacaatc tccagcgtgt acatgccgga
    45781 atagaacacc agcaactgaa catacagcgg cgtaccgcga aaaatatagg taaataacca
    45841 gattggaaac tggatgtatt tattacttga gacgcgacca atcgccagaa acagcgccag
    45901 aactccgcct atcactaccg acaaaatcag cagccacagg gtgatcgcca cgccagtaaa
    45961 gcgataaccg tcagtccaca gcagtggttt ccagtattca tgtaagattt cgatcacagg
    46021 tcagccctct tcacacccac ggagtagcgg cgctcaagga acagcagcac accattggaa
    46081 acggtggtga aaacaagata aatcacgcca cagacgatag cgaaatagaa cggttcccag
    46141 gtacttttgc ctgccagctg cgtggctttg accacatctt ccaggccgag taacgaaacc
    46201 agtgcggtag atttgaggat cacctgccag ttgttgccaa tgcctggcag ggcgtaacgc
    46261 atcatcgccg gaaacatgat ccgccgaaac acttgcccac gagtaaaacc gaacgccgtc
    46321 gccgcctcta tatgtccttt cggcactgcc ataaacgcgc cacgaaacgt ttcggtaaag
    46381 taagctccgt agataaaacc gagagtgata atacctgcga ccatcggatc aatatcaatc
    46441 tgcccgacgc ccatcgcctc cgtcaccgtg tttagtgcaa tctgcaaacc gtagaaaatt
    46501 agcagcatca acaccaagtc cggcacgcct cgaatcagcg tggtgtaccc ttcgaaaatc
    46561 agccccgaaa gccgattttg cgagagctta ccgccagcgc caattaaacc gatgattaca
    46621 gcgagcacta cagagctgat agccagctcc agcgtgacga gcgccccctg taaaataaca
    46681 cctgaaaacc catacaacat gctgcctgtc ctgtcgtgtg tggtgaatta ccgcctcttg
    46741 tctccctccg gttacccgaa gggagcggac tttctttcac cgatggcgat taaccaccat
    46801 aaacatcaaa atcgaagtac tttttcgcta atttctcgta agtaccgtca gcgcgcattt
    46861 cggcaaaggc tttgttcagt gcctcgcgca gttcgttatc ttctttacgc aggcccatgc
    46921 ctgtccctac gccaaacagt ttttcatctt taacagacgg gccaccgaat ttgtaatctt
    46981 tgccgactgg ctgcttgagg aaaccttcgc tggcagcgac ctcatcctgg aacgcggcat
    47041 caatacgtcc ggcagtcagg tcagaataaa tgttgtcctg cccctgatac gagacgattt
    47101 caatgccttt tggtgcccag tgttcgttac cgaacgtctc ctgggtggtg ccctgcaata
    47161 cgcctacccg tttgcctttc agcgattcga ctgtcggctg aatgtcggaa tttttcgcca
    47221 ccaccagacg agaatcggca gcgtacagtt tgtcggtgaa ggctatttct tgctgacgtt
    47281 tttccgtaat ggaaagcgat gacatgatgg cgtcaatctt cttcgctttt aaggacggga
    47341 ttaacgcatc cagtgggttt tcgacaaacg tacattgcgt attgatgcgt ttgcacaact
    47401 ccttcgccag atcgatatcg aagccaacca gttcaccttg tgaatttttt gattcaaatg
    47461 gcgcataggt cggatcggta ccgatgcgga tgttttgcgg aatcgcagca aacgccgcag
    47521 ttgcgctgga gaaggccaga accagagaga gcgatagcac cagttttttc ataatgtcct
    47581 caactgactg tctttttata gggaatattt acaggtttgt tacacttatc gtgccataaa
    47641 attgaccatc aaggcaaaat atcccgcccc gttagacgta ttatttttca ttaaaaatgc
    47701 ttaagtatgc atttcaatgc accacgatag tgcaacaaac ctggcgtgca ccatgatgat
    47761 cattccatca ggtacagctt ccctgcgacg tatcagtcac cgtagacatt aaagtcgaaa
    47821 tactttttcg ccatcttgtc gtaggtgccg tcctgacgca gctcgttaag cgccttattg
    47881 aaggccgccg tcagttcagc atcatcttta cgcagcccta caccggtgcc gtcaccgaag
    47941 tattttttgt ctttcactga tgggcctgca aaggcgaaat ctttaccggc gggttgctta
    48001 aggaaacctt cgctggcagc aacctcatct tgtaacgcag catccaggcg tcctgcagcc
    48061 agatcggaat agaccaaatc ctggttagca taggccacca catcgacccc cttactgcgc
    48121 cagttatcat tggcatacgc ttcctgggtt gatccctgca gcacaccaac atgtttacct
    48181 ttcagtgaat ccagcgttgg ctgaatcggt gaacctttgg cagcaatcag acgagagtct
    48241 gcggcgtaca gcttgtcgga gaaagcaatc tcctgctgac gtttatcggt aatggagagc
    48301 gacgaaataa tggcgtcgat ttttttcgct ttcagtgagg ggatcagcgc gtcaaagtcg
    48361 ctggcaaccc aggtacattt cacctgcatc cgtttgcaca tctcgttacc gagatcgata
    48421 tcaaagccaa caaaatcacc tttagcatct ttcgacgaga acggcgcata ggtggtatcg
    48481 gttccgatac gtaccgtctc cggtagcgcc gcatagctgg aagccgctgc ggagagaccg
    48541 actaacaaag acagagcgag aatcgacttc ttcatacata accctcaagt gaaaatggct
    48601 tttttatgtt gtgtattgtg ttgtgtgttt gcaggctctt tcatgcaggt cttatgccat
    48661 cttgacgaca gcagtaacat tcaacgttaa atatgttaat aagacgttgc attattgtca
    48721 tgaagttgaa gatagcaggt atagcggttg aatcgcagcg tttcgattga atggcagaaa
    48781 caaaatgtcg aggatttgat cgcggttgca aaattgccct gaaacagggc aacagcggag
    48841 ttatgcgccc tgccagcggg caaagagatc ttcaggaagg gttattgcaa actggtcaag
    48901 aacacgatta accgtctgat ttatcacatc atcaagggat tgtgggcgat gataaaacgc
    48961 cggaacggga ggcataatca ccgcaccgat ttctgccgcc tgagtcatta aacgcagatg
    49021 gcctaagtgc agtggcgttt cacgcacgca gagcaccaac gggcgacgct ctttcagcac
    49081 cacatctgcc gcacgggtca gtaagccatc agtatagcta tggacaatgc cggacagggt
    49141 tttgattgaa cagggtaaaa tcaccatccc caacgtctgg aaagaaccgg aagaaatgct
    49201 ggcagcaata tcgcgcgcat cgtgcgtcac atcggctaat gcctgcactt cgcgcaggga
    49261 aaaatccgtt tcgagggata aggtctggcg cgctgcctgg ctcattacca gatgcgtttc
    49321 gatatctgtg acatcgcgca gaacctgtaa taaacgcacg ccataaatcg cgccgctggc
    49381 accgctgatg cctacaatga gtcgtttcat aaatagttgc cctgtcagac ttgcaggcag
    49441 actttgcagg atttcggcgg gagttgcaag tcagggtgcc agaacggcac cctgagcgaa
    49501 ggcatcatcc ttcgttatgc atttcgagat tttccacttc gttctgacgt tgcactgctt
    49561 tggcgtcgtc attacgtaac gtatcgagga aatcgaggta gccctgatca acatctttgg
    49621 tgacgtagac gccgttgaat accgagcatt caaactgctg gatatccggg ttttcagcgc
    49681 gaacggcttc gatcagatcg ttcagatcct ggaaaatcaa cccgtcagca ccgatgatct
    49741 ggcgaatttc atctacttcg cgaccgtgag cgatcagttc cgtggcgctc ggcatatcga
    49801 taccgtaaac gttcgggaag cgaatttccg gtgccgcaga agcgaggtac actttcttcg
    49861 ctccggcttc gcgtgccatc tcgataatct gctcagaagt ggtgccacgg acgatggagt
    49921 cgtcgaccag caggacgttt ttatcgcgga actcggcgcg gttggcgttc agtttacggc
    49981 gcacggactt acgacgcagc tgctggcccg gcatgataaa ggtgcggcca acatagcggt
    50041 ttttaacgaa gccctggcgg tacggcttgc ctagaatacg cgcaatttcc agcgcgatat
    50101 cacacgaggt ttccgggatc gggatcacca cgtcgatttc cagatcttcc cattcgcggg
    50161 caattttctc gcccagtttc gtacccatat tcacacgcgc gctgtaaacg gaaattttgt
    50221 cgatgaacga gtccgggcga gcaaagtata catactcaaa caggcacgga ttgctgaccg
    50281 gattgtcagc acattgacgg gtaaacaact gcccttcttc agtgatgtaa atcgcttcgc
    50341 ccggcgcgac gtcacgcagg aaatcaaagc ccagcgtatc gagcgctacg ctttcggaag
    50401 cgaccatata ttctgtacgg ttctcgtcaa tatcacgttt tcccagtacc agcggacgaa
    50461 tcccgttagg atcgcggaaa gcaaccatac cgtggccgat gatcatcgcc acacaggcat
    50521 acgcgccgcg gattaagcgg tttgtagcag cgatggcagc gaaaatattg tcggcttcca
    50581 gcgggtagtg gcggaagttg tccagttcgc tggcaaagat attaagcaga atttccgagt
    50641 cggaagtggt gttgatgtgg cggcgttttt cttcaaacag ttttttacgc aactcgtgag
    50701 cgttggtcag attgccgttg tgggcaagcg taatgccata cggggagtta acgtaaaacg
    50761 gctgcgcttc agaggcgctg gagctgccag ccgtagggta acgcacatga ccaatgccca
    50821 tattgccctg caaacgctgc atatggcgag cttcaaatac atcgctcacc agcccgttcg
    50881 ctttacgcaa acggaagcag ttattggcat ctatggtgat gatgccggcg gcatcctgac
    50941 cgcgatgctg aagcaccgtt aaggcatcat aaatcgactg gttaaccggc ataacaccgg
    51001 cgataccgac aataccgcac atacgtcttt ttcctcgtta agccacatct cagagcactt
    51061 acgctctggg caagaaactt gacgagcttt gcagataatc aaaaaaccat ctgatgataa
    51121 aactgaactg cgggatcagc tgtgatttgc tccagtcttc gcttttcgac accccggtaa
    51181 aggagtcgag aaagaagaga atggcagcaa caatcaacac accgcgcaac gcaccgaaac
    51241 agacgcccag cacccgatcg gtgcctgaca accccgtttt ctccaccaac tggcctatca
    51301 cgaagttcac gatagcacca acaatcaggg tagcgataaa cagtaccgcg atggcaatcc
    51361 catttcgaac cagttcgtct tcaaagcccg taaaccagac tgacaggtaa gtgtagtaat
    51421 gactggcaac aaagaaagca caaccccatg tcaccagcga taacgcttca cgaacaaagc
    51481 cgcggatcag gctaaccaga gaggaaaaag caatcaccgc gattatggcg taatcaatcc
    51541 agaccatatg tgtcccacga ttttacgccc tgtcatcctg ttcggggcgc attctaacag
    51601 aaaaagaaaa cgtttgcgta gggatttcct tcccgcgcat caataaaaat ggcgctgaaa
    51661 aaatattcaa cgccatcgac tttttatgcc tttgcggcat cgggcaatgc gtgtcggatg
    51721 cggcgtgaac gccttatctg taccagataa gacgcgttag tatcgcatca ggcaagacca
    51781 tattaattcg gtgtataacc cattaccacg ccgcttaagc cagaaagttg cttcaactca
    51841 cccagcgaac ctttcagctt atctttcgag gcatccggcc caaccagaat acgggtaatt
    51901 ttaccctgca ctggcgtgga tggcgacgta taaacccgat aaccggcacc gcgcagctta
    51961 ccgacaatct cattcacttt atcggcattt ttcagcgcgc ccagttgcac aacataagct
    52021 ttacccgttg gcgctgcttt ttcttccacg accggcttcg gttctggctt cggcgcaggt
    52081 ggtgcttcaa cctttggctt aggcggctct accggtttcg gcttcggtgg gacgaccggt
    52141 gcaggttccg gttcaaactc ggtgttattg gctgcaatag tggccggatc gagcgacggt
    52201 gctgcggcat cacctgcccg cacctcttcc gctgcgcctt ccggcggctg cgtcggcaac
    52261 gcctgagtgg ctgctggcat catatcaggc tcatcgcgat cgcccgcttt cggcaccagc
    52321 gggatagccg cgaactcatc ctgataatgt tttttctgcc cgtccagcag ccctggaagt
    52381 acaatcaccc ccagcgccac cagcacgatc gtgcccacta accgattctg aaacttactt
    52441 gccaccgctt ctcctcgcgt caatcacttc catgacatgt gcgaccgtgt ggaacgaacc
    52501 acacaccagc acggtatctt ccgctttagc gtccgccatt gcggcatccc atgcctgcgc
    52561 cacgctatca aatgatttgc cgttacccaa atgctcaagc agttgttctg ccgtggcacc
    52621 gcgcggccct tccaggggcg cacaatacca gtcatcaacc acgcttttca accaggccag
    52681 agttccggca atatctttat catgtagcat accgataacc gccagcacgc gcccgttttt
    52741 cggtagcgct ttcatacgcc cggtgagata ttccgccgca tgtggattat gcgcgacatc
    52801 aaaaataacg cgtggcgact cgctcacaat ctggaaacgt cctggcaaaa ttgcgctggc
    52861 aatcccgtcg cgaatggcat tttcactgac ctccagcccg ctggcacgca gtgccgccag
    52921 cgccgttgcg gcattcggtt gcgggacaag cggcaacggc agattttcca gcgtgccgtg
    52981 agcatcgcta aacgtccagt catgatcggt gacggaatag ttccactcaa cgccccgacg
    53041 ttgtagcatt gcaccttttt cctgcgccac atcagcaatg gtagaaggca tttccggctc
    53101 accgacaatt gccggttttt cgctgcggaa gatgcctgct ttctcgcgac caatactttc
    53161 gcgatctggc cccagccagt cggtatgatc cagcgcaatg ctggttacta ccgcgacatc
    53221 ggcatcgaca atattggttg cgtccagacg accgcccagc cctacttcca gaatcaccac
    53281 gtcaagttgt gcctgtttga acagccacaa cgccgacagc gtaccgtact cgaaataggt
    53341 caaggaaata tcaccgcgtg ccgattcaat ctccgcaaaa gaggcggtgt gggccgattc
    53401 cggcaattcc tggccctgca cgcgtacgcg ctcggtataa cgcaccagat gcggcgaact
    53461 gtagacgccc actttgtacc ctgccgccat cagaatcgac tccagcgtac ggcaagtggt
    53521 gcctttgcca ttcgtacccg caacggtaaa cacaaatggc gctggtttca gaacgccaag
    53581 acgcgccgcg acctggctca cgcgctcaag gccgagatcg atagttttac tgtgcaggtt
    53641 ttccagataa gaaagccacg aagccagagg cgacgcggct tgaggagtgc gtttgataat
    53701 catggtatcc gctgattcgt tacggtgaga atagcaaaag ggcagagcca gtggccctgc
    53761 ccttatcagt tatcaggcct caggttcctg atctggtacc ggtggcacca ctacgccttc
    53821 acgcggcgct tcaggattcg gcgctggcag attcatcaac ttcgccagaa tactcgccag
    53881 tttcaggcgc atttccggac gacggacgat catgtcgatc gcgcctttct cgatcaggaa
    53941 ttcactgcgc tggaatcctg gcggcagttt ttcgcgaacg gtctgttcga taacacgcgg
    54001 accggcaaag ccgattaacg ctttcggttc agcgatgttg agatcgccca gcatagcgaa
    54061 acttgcagaa acaccaccca tggtcgggtc ggtcagcacg gagatgtacg gcaagccgcg
    54121 ctcctgcatt tttgccagcg ccgcagaggt tttcgccatc tgcatcagcg acatcagtgc
    54181 ttcctgcata cgtgcgccac cagaggcgga gaagcagatc agtgggcagt tatcttccag
    54241 cgcctgctca acggcacgca cgaaacgtgc acctaccaca gaccccatgg aaccgcccat
    54301 aaaggcgaac tcgaatgccg cagcgacaac cggcattcca tacagggtgc ctttcatcac
    54361 caccagcgca tctttttcgc cggtttcttt ctgcgcagat gccagacggt ctttatactt
    54421 cttggagtca cgaaacttca gcacatcttt cggctcaagc tcgctaccca gctccacaag
    54481 gcttccttca tctaacaggc tatgcaggcg attacgcgct gtcatacgca tgtgatggtc
    54541 acacttcgga cagacctcaa gattacgttc cagctcagcg cggtataaaa cctgaccgca
    54601 gctatcacac ttagtccaca ccccttcagg aatgctcgcc ttgcgggtgg gagtaatgtt
    54661 gcttttaatt cgttcaatcc agctcattag ggacctttct gtctgaacct ggttcgatgc
    54721 cagttttatc tttggggacg cataatgcca tttttgcccc caacagacca tgaatgttgc
    54781 acattaaaac ataacagccc gaaactttgg ataaaaaagt ggtcgaaccg cggagttact
    54841 ttttattttg cggcgcgtgc cgcagcgcgt ttgtggcgaa taaattcgaa aacacctggc
    54901 aatattgaaa caaaaataat gccaacaatc atcagtttaa ggttgctctg aatgaagggg
    54961 agcgttccga agaaataacc cgcgtaggta aaaatcagta cccatagcaa cgcgccgatg
    55021 acgttaaaag cggcgaaatg acgatacgac atgtggccca ttcccgcaac gaacggcgcg
    55081 aacgttctga cgatcggcac aaaacgggcg agaataatcg ttttgccgcc atgtttctca
    55141 taaaactgat gagtcttatc aagataactg cgacgaaaga ttttcgaatc cggattactg
    55201 aacagtttgt caccaaataa ccgtccaatg gtgtagttca ccgcgtcacc cacaatcgcg
    55261 gcgatcagca tcagcaccac catcatatgg acgttgagat cgttggtttc cagtgatgcc
    55321 aacgcaccgg caacaaacag caatgaatca cccggtaaaa acggcgttac caccagaccg
    55381 gtttcacaga acaaaattaa gaacaaaatg gcataaaccc agacgccata ctccgcgacc
    55441 agttctgcca gatgcacgtc aatgtgcagg ataaaatcga tgaggaaata aatcaggtcc
    55501 atatttgcct atgccttgta ctcgtcatct ttcaggctgt aactacgttg gctacagcct
    55561 gaaatgttcc gagcgttatt ctcgttagtc cgccagaaat agcgggccca ttggcggttt
    55621 tggaagatca tatcggtcag ggtaatccac cgcgaccaga tatagccctt ccgcttttgc
    55681 cgttgctgcc gccagcgttc tgtcctttgc cgccagcaac tctgctatcc agctctccgg
    55741 ctggttgtgg gcaccgactt ccatcaggct gccgacaata ttcctgacca tatgatgtac
    55801 aaaggcattc gctttgatat ctaccaccac ataaggaccg tgacgcgtga cattaatatg
    55861 cataacgttg cgccacgggg tccgggactg acactgcacc gcacggaacg aggtgaaatc
    55921 attctcgccc agcaaacatt gcgcagcccg atgcatccgt tcagcgtcca gcggttcgta
    55981 aaaatgggtt actcctttac tcaataccgc cgggcgcagc cgatgattgt agatgatgta
    56041 gcgataacgg cgagccgtgg cgctaaatcg ggcatgaaaa tcatcaggta cagctttaac
    56101 ccaacgcacg gcgatgtcac caggtaaatt cgcatttacg cccaacgtcc acgcagcgtc
    56161 tttgcgctgc gcggtcgttt cgaaatgcac aacctgcccg gtgccgtgta cacctgcgtc
    56221 agtacgcccg gcgcagaaga cggtgatggg ttcgttcgcc acctgggaga gcgccttttc
    56281 cagcttctcc tgcacactgc ggacttcgtt ctgccgttgc cagccgtaat acttactgcc
    56341 gtcgtactca atgcccagcg caattttata aactggcggt tgttgctggt cggacattag
    56401 tacagatact cctgcaccag tttctcggcg atttttactg ccatcagcgc gccgccaaag
    56461 cgaacgttat cggccaccga ccagaactga acttgctccg gcataccgta atcattacgt
    56521 acgcagccaa cggaaagatg cggcgtacca gaagcatccc ccacctgagt cgggaattcg
    56581 ttctcttcag agagcacaat atcttcgccc tgagcaaacg catcacgcgc ttcttctgcc
    56641 gccagcggac gcagtgcttc aaagttgacc atctgggcat gaccatagaa taccggtgcc
    56701 tggacgacgc ttgccgaaat catcagccct tcgtcctgca ggattttgcg tacttcgtca
    56761 acgatacgac gttcttcacg cacgctacct tcgctatccg gcagtaacgg cagcatattg
    56821 aatgccagtt gacgcccgaa gaaatcttct tcgtcaatcg gaatgccatt gagtaattta
    56881 gcactctgcc ccgctaacgc atcgaccgct tttttaccct gggcggaggc tgaaatcagg
    56941 ctggtaacgc tgatacgtga taaaccgccc tgatcaatta gcggtttcag tgccgccagc
    57001 agctggctgg tcagactgtc tggtacggcg atgacattcc ggttgcggta atcggtcagt
    57061 acaaacgggt ttacttccgg caccaccagc ggtacgtcgg gttcgagagc aaacaatccg
    57121 ctgctgtcga tcaccagaca acctgagttg gtcgcttctt caacccaggc agcggtagct
    57181 tctttgcctg caacaaaaaa cgccagctgc gcctgcgtcc agtcgaattc agcggcatcc
    57241 tgcacggtga ttgtcttacc accaaagcgc agttgttcgc ctgcgctttc gttacgtgcc
    57301 agtgcataaa tttccccaac cgggaactga cgttcagcca gcgtttcaag cagggcttcg
    57361 cccacagcgc cagttgcgcc caggacggca atgttccagc cttcagacat ggtggtttac
    57421 tccagaaata gcaaagctcc ctgccaatat tacagcagag agcatgaaga agagattaac
    57481 gtgccggatg atggacggcg ttaaaaccca gtttacacag caatgatgcc gcactggcgt
    57541 catcacaaat tacatacaga gatgaccatt cacggcgctc aagatagttt ttgcgcagct
    57601 tatcgaactc acccggtatc ccggcgactt tacgcagtgg cgcgtcatcg cggcgcacat
    57661 catacaccaa atgcaccagc cttttcagcg ttggctgatc gagcgggcca tgcagcgtaa
    57721 tgcgaccaaa ctctggcgcg ggcagtaatg tatccagcgc aacgtgctgt tcatgcccaa
    57781 taaacttgct ataagcttca aacacttgcg tagtaccgcg tgctttacct tccagggtat
    57841 agcctgcgat atgcggcgtg ccgatatcca cttttttcag caattccacg ttaagttccg
    57901 gttcgccttc ccagacatcc agcactacgc ttaacttctg gccttcgttc aggcaggtca
    57961 gcagggcggt attatcgaca actgcgccac ggcaggcgtt aatcagaatc gctcctggtt
    58021 tcaggctgcg gatcagtttt tcatccgcca gatgcagcgt tttgtacggc ccgtctttaa
    58081 agagtggcgt atggaaagtc agaatatccg catgctgaac taattcatcc agcgagcgga
    58141 aatcaccctc atctccacgg tcggcgcgag gcggatcgca aagtaaggtt ttaatcccta
    58201 acgcttccag tcgtgcctgt aaacggcgac caacgttacc aacgcccacg atccccacgg
    58261 tacgctcgtg cagtgaaaat ccatcgcgtt cggcaagcat cagcagggag gagaaaacat
    58321 attccaccac cgcaatcgca ttacagccag gtgcagcgga aaaaccaatt cccgcctgct
    58381 ttaaccacgc ttcatcgaca tggtcggtcc ccgctgtggc agtgccaaca aatttaatgg
    58441 gttttcctgc cagcaaagat tcattcactt tcgtgaccga acgcaccatc agcgcatccg
    58501 cgtcggccag ttgagcgacg ggaattggac gcccgggaac cgcggtcacc tcacccaaac
    58561 ggctaaataa gtcgcgggca taaggcatat tttcatcaac aaggattttc acgtttgtgt
    58621 tacctgtatg agacgagagt taaccggaca agtgtgccat aatctcgcgg ccaggcatac
    58681 ttgcgaagat ttcaggtata aggatacgta atgatacaac ctatttccgg ccctcctcct
    58741 gggcaaccac caggtcaggg agataacctg ccgtctggcg cgggcaatca gcctttatcc
    58801 agtcagcaac gtacttcgct ggaaagctta atgacgaaag tgacctcact gacgcaacag
    58861 caaagagcag aactgtgggc gggtatcagg catgatattg gtctgtcggg agattcaccg
    58921 ctgctttcgc gtcacttccc tgccgctgag cataatctgg cgcaacgtct gttggccgcg
    58981 caaaaaagcc attctgcccg ccagctttta gcgcaattag gggagtattt acgtctgggg
    59041 aataatcgtc aggcggtcac ggagtatatc cgtcataact ttggtcagac gccgcttaat
    59101 cagctctcac cggagcaatt aaaaaccatt ctcaccctgt tgcaggaagg gaaaatggtt
    59161 attccgcaac cacagcagcg cgaggcaacc gaccgtcctt tattaccggc ggagcacaat
    59221 gcgctcaaac agctggtgac caaacttgcg gcggcaacgg gagaacccag caaacagatc
    59281 tggcaatcga tgctggaact ttccggggtg aaagatggcg agttaattcc agcgaaactg
    59341 tttaaccatc tggtgacctg gctacaggcg cgtcagacgc taagccagca aaatacgccg
    59401 acgcttgaat cactacagat ggcgctaaaa caacctttag atgccagtga actggcggcg
    59461 ttatcgacat atatccaaca aaaatatggc ctttctgcgc aatcatcgct ttcttctttc
    59521 caggccgagg atattcttaa ccagctttat caacggcggg ttaaagggat tgaaccgcgt
    59581 gatatgcaac cgctgcttaa tccttttcca ccgatgatgg acacgttgca aaatatggca
    59641 acgcgtcccg cgctgtggat actgttagtc gcgattatcc tgatgctggt ctggctggtt
    59701 cgttaacccc gacgaaacga cagtatcgtg acaataattc ccagcaccgc agagatcgcc
    59761 ccggcaagaa ataccgaaga gtaaccaaac gtggtcgcca gcattcccgc aagcggcccg
    59821 gagacgccga gggcgatatc ctgaaacgcg gcgtaaccgc ccagtgcggt gccgcgaacg
    59881 tgtgagggga cgcgtttaac cacctccacg cccagcgcag gaaagataag cgaacatccg
    59941 gctccagtta acgccgcgcc tgctaatgcg acccacgccc ctggggcttg ccagagcagc
    60001 aacaagccca ccgtttctac aagcagagag acgatcgcca ctttcacgcc gccaaaacgg
    60061 tccggcatcc agccaaacat gacgcgcatc acgacaaatg cgccgccaaa cgcggtaaga
    60121 gtaaagcccg ccatcgccca tcctttgctg gcaaagtaga gcgaaacgaa agtcccgata
    60181 accgcaaaac caacgccttg tagtgccaga cctaaccctg gtttccagat aagcccgaca
    60241 acgctccaca gcgatggacg ttctcccgcc agggccggta ctttgcgcac tgtgccgtta
    60301 caggcccacg ccagtaaggg taatgccatt gtggtgagcg ccagtgcggc aaaaccgtaa
    60361 tggctatgaa tcaacaggcc aagcggcgca ccaacagcga gggcaccgta aatcgccatt
    60421 ccgttccatg acatcacttt gccggaatgt tttggcccaa cgatgcccaa tccccaggtc
    60481 agagcccccg tcagtaactg gctttcacca aagccaagaa tcaaacgtcc aacgaccaac
    60541 agagcaaatt tgaacggtgc ggagacgggc aaaatcgccg ccaacagcag tgcgcccccc
    60601 gccagaccac aagctaacat cccctgaagc gccgaacgtt ttgcgccata ttgatcggcc
    60661 agtcgcccgg cgtaaccacg cgtcagcacc gtagccaaaa actgaatgcc gacggcaatc
    60721 ccgaccatgg tgttgccata gcccagctca tgatgaacaa acagcgggat aaccggcaac
    60781 ggtaaaccta cggtcatgta ggtgagaaaa accgcaaaag cgatgcggaa gagcgaaaaa
    60841 ttggcagaag atcgtgtttc ggtttggctt acagcagtca tgcattactc cagaatgcag
    60901 cgcaaggcga ggagtatccc cgtctcatct ctctggtttc agggttacgg tgcgttggca
    60961 ggatttaacg catacgtctt tttagcagga tatcgacaaa gcgggaagtt tgcctggaac
    61021 tggcggcgat tgtcaatgat gtgaaaaagg gaaccatcgg gttccctttt gcgttagtgc
    61081 cggaggccgc attggtgcgt aaccactccg aattaatctt tcagcttgcg cattaccagc
    61141 gtggcgttag tgccgccgaa gccgaagctg ttagacataa cggtggtcag ttcgcgatcg
    61201 gtcgtttcgg tcacgatgtt cagacccgca gcctgctcgt ccagctcttc aatgttgatg
    61261 ctcggggcga taaaaccgtg ttccagcatc agcagagagt agatagcttc ctgtacgcca
    61321 gcagcgccca gtgagtgacc ggtcattgct ttggttgcag aaatcgccgg gctcttatcg
    61381 ccgaacactt cacggatagc tgccagttct ttcacgtcgc caaccggagt cgaagtacca
    61441 tgagagttca ggtagtcgat tggggtatca acaccgtgca tcgccatctt catgcagcgc
    61501 actgcacctt cgccagacgg agcaaccatg tctgcgccat cagaggttgc gccgtagcca
    61561 acgatttcag catagatgtg agcaccacgc gccagcgcgt gttccagctc ttcaaccact
    61621 accataccgc caccgccagc gataacgaaa ccgtcacggt gagcgtcgta agtacgggag
    61681 gctttttccg gggtgtcgtt gtatttagta gacagcgcac ccatcgcgtc gaattcgcaa
    61741 gccatttccc agcacagctc ttcgccgccg ccagcaaaca cgatgtcctg tttgcccagt
    61801 tggatctgct ctactgcgtt accgatacag tgtgcggaag tcgcacacgc ggagctgatg
    61861 gagtagttaa cgccgtgaat tttaaacggc gtggcgaggc aggcagaaac gccggatgcc
    61921 atcgctttgg tgaccacata cgggccaacc gctttcaggc ctcgcgggcc gcgcattgcg
    61981 tcagcgccga aaacctggaa acgcggggaa ccgccgccgg aacctgcaat caggccaacg
    62041 cgcgggttat tctggtaagc ttccggggag aggccagcat ctgcaattgc ctgctccata
    62101 gaaaggaatg cataaatgga tgcgtcgctc ataaagcgca caactttgcg gtcaatgagg
    62161 ccagtggtat ccagttttac gttgccccag acgtggctac gcatgccgga atccttcagc
    62221 tcctgagaga aagtgatccc tgaacgtcct tcacgcagag atgccaggac ttcctgctgg
    62281 ttattaccga tgctggaaac aatgcccagg ccagtaatca ctgcacgttt cattcaatac
    62341 ctctgtaagt cgcacataga gtaagtttcg catgcacaat agcgtacact tgtacgccga
    62401 acaagtccga tcagccaatt aatagagaaa tttgcgcagc ctggcacaca tcgctaagat
    62461 cgagccaccg cctgtaagac gagtaactta cgtgaaacac tacgccatac aacctgccaa
    62521 ccttgaattt aatgctgagg gtacacctgt ttcccgagat tttgacgatg tctatttttc
    62581 caacgataac ggactggaag agacgcgtta tgtttttctt gggggtaacc aattagaggc
    62641 acgttttcct gagcatccac atcctctgtt tgtggtagca gagagcggct tcggcaccgg
    62701 attaaacttc ctgacgctat ggcaggcatt tgatcagttt cgcgaagcgc atccacaggc
    62761 gcaattacaa cgcttacatt tcattagttt tgaaaaattt cccctcgccc gtgcggattt
    62821 agccttagcg catcatcact ggccggaact ggctccgtgg gcggaacaac ttcaggcgca
    62881 gtggccaatg cccttgcccg gttgccatcg tttattgttc gatgaaggcc acgtgacgct
    62941 ggatttatgg tttggcgata taaacgaact gaccagccaa ctggacgatt cgctgaatca
    63001 aaaagtagat gcctggtttc tggacggctt tgcgccagcg aaaaacccgg atatgtggac
    63061 gcaaaatctg tttaaggcca tggcaaggtt agcgcgtccg ggcggcacgc tggcgacctt
    63121 tacgtctgcc ggttttgtcc gccgcggttt gcaggaagcc ggatttagca tgcaaaagcg
    63181 caaagggttt gggcgcaaac gggaaatgct ttgtggggtg atggaacaga cattacccct
    63241 cccctgctcc acgccgtggt ttaaccgtac gggcagcaac aaacaggaag cggcaattat
    63301 tggaggcggt attgccagcg cgttgttgtc actggcgcta ttgcgtcgcg gctggcaagt
    63361 aacgctttat tgcgcggatg aggctcccgc actgggtgct tccggcaatc gccagggggc
    63421 gctgtatccg cttttaagta aacacgatga agcgctaaac cgttttttct ctaatgcgtt
    63481 tacttttgct cgtcggtttt acgaccaatt acccgttaaa tttgatcatg actggtgcgg
    63541 cgtcacgcag ttaggctggg atgagaaaag ccagcataaa atcgcacaga tgttgtcgat
    63601 ggatttaccc gcagaactgg ctgtagccgt tgaggcaaat gcggttgaac aaattacggg
    63661 cgttgcgaca aattgcagcg gtattactta tccggaaggt ggctggctgt gtccggcaga
    63721 actaacccgt aatgtgctgg aactggcaca acagcaaggt ttgcaaattc gttatcaaca
    63781 tcagttacag gatttatccc gtaaggatga cggttggttg ttgaattttg caggtgatca
    63841 gcaagcaaca cacagtgtag tggtactggc gaacgggcat caaatcagcc gtttcagcca
    63901 aacgtcgtct ctgccagtgt attcggttgc cggtcaggtc agccatattc cgacaacgcc
    63961 ggaactggca aaactgaaac aggtgctgtg ctatgacggt tatctcacgc cacaaaatcc
    64021 ggcgaatcaa catcattgta ttggtgccag ttatcatcgc ggcagcgaag atacggcgta
    64081 cagtgaggac gatcagcagc agaatcgcca gcggttgatt gattgtttcc cgcaggcaca
    64141 atgggcgaaa gcggttgatg tcagtgataa agaggcgcgt tgcggtgtac gttgtgccac
    64201 ccgcgatcat ctgccaatgg taggcaacgt gcctgattat gacgctacac tcgtggaata
    64261 tgcgtcgctg gcggagaaga aagatgaagc ggtaagcgcg ccggtttatg acgatctctt
    64321 tatgtttgct gctttaggtt cgcgcggttt gtgttctgct ccgctgtgtg ccgagattct
    64381 ggcggcacag atgagtgaag aaccgattcc gatggatgcc agtacgctgg cggcgttaaa
    64441 cccgaatcgg ttatgggtgc ggaagttgtt gaagggtaaa gcggttaagg cggggtaatc
    64501 tgctctggca ttgcttgccg gatatggcgt gaacgccgca tccggcacat tacttactgc
    64561 tgtgacgcct gctgaaacag gtgttcccac atatcggtca ccagcgcctg gtcacgcggc
    64621 gacaattcac cggcaccaat agctttttcc agactctggc taacggttgt atgtaccgcc
    64681 tgagcggagt ggtcgtcacc actttccagt tctgcgatgg ctaatgtcag gtggccacgc
    64741 aaatacccac tggcaaacaa ctcatcatca ctggcatagt caaccatacc gtcgattaat
    64801 gccagaatgc gtgattcaaa ctccgcgatc atcttctttc ctcattgtaa aacgtggcgc
    64861 tggcttcagt tgagcgcttc cggccacggg aactgttccg ccgtaagttc cggcgtgtga
    64921 taataatttt gtagtgcttt aatgaagcgt gccggacgtt cgggaatgcc gtttgccaga
    64981 taatccatca cctgcgcatg tacccgccgt tgaaacacca cacgatccgg ttcgaaatcc
    65041 ccttccagat tgtcgcagct aacattaaac ggatatcccg ccgccacgca gaacaaccag
    65101 tccagcgcct gcggcttcac ttcaacatct tcaaactggc tttgcgtttg ggcatcgcgt
    65161 ccgtccgggc aataccagta accgaaatca accagctcac ggcgcgcttt cccggcaata
    65221 caccagtgcg aaatctcgtg gatggcgctg gcataaaagc catgagcgaa gacgattctg
    65281 ttatacggtg cttccgcatc agcaggaaga tagatcggtt cgtcgtcgcc tttaatcaga
    65341 cgggtattaa attcatcggc aaagcagcta ttaaaaatct caatcaactg ctcatagtgg
    65401 tgtgtactgt tcattagttc atccccaacc agtggaggat ctcctgtccg tggctatcgt
    65461 aaagtaattt ggcactcatc accgccgaga cgataacgat catcgggcgg atcagctttt
    65521 gtcctttgct caacaccagt cttgacccca tgcgcgcgcc gaggaactgt cctaccaaca
    65581 tcacaaatcc tgtcgcccaa atcactttgc cgccgagaat aaacagcagc aaaccaccga
    65641 tgtttgacgt tgcgttgagt aatttggcgt gggccgtggc tttggcgagg ttgaatccgc
    65701 acagcgtaac aaaggccagc gcgtaaaacg acccagcagc cgggccaaag aatccatcgt
    65761 aaaaaccgac gcagcccccc gccaccagcg caaatggcag tccatacatc cggcgctggc
    65821 gatcttcttc tcccagtttt ggcatcagca aaaaatagag gccgatacaa atcaccagga
    65881 tcggtaaaat ctgccgtaag acatcggcct gaacatactg caccaacagc gcgccgctca
    65941 ttgagccgac aaaggtcatg gcgatattga gtttctgatc gcttaagcta accactttgc
    66001 gccgaataaa gtagatagtg gcggaaatag agccgccgca ggcttgcagt ttattagttg
    66061 ccagcgcgtt agccggagac atccccgccg ccatcaatgc cggaatggtg agtaacccac
    66121 cgccaccggc aatcgagtcg ataaatcccg ccagcatggc gacaaaaaag agaactccca
    66181 gcaacagcgg ggaaaccata aacaggctat taaacgtttc cattagatca cgtgctcatc
    66241 cagtagcgcc tggcaggaag gcggcaacgg aggcggtgtc ttcttctcag gctttgttgt
    66301 tcccggtttt ggtggttcaa accagctttg cagttctgcc ccgcaaccat cgcctggtgg
    66361 cggtaaaggt tgatcttcac actccagact atcggcagga caacgtaatc gtacatgcat
    66421 atgcgcgcga tgctggaacc agggtcgcac tttacgtaac cagtcgcgat cggtgcccgc
    66481 atcaaggcaa agttgttgtt taatcgccgg attaacaaaa atgcgcgtga cgtctttgtc
    66541 ctgggcggcg agtttgatca agctgaaaat ttctggcttc cacagcgcgg ggacaacgtg
    66601 tttaccgtcg cgggaaacta agtccagagc ttgcgggcgc aagagctgcg cggaggtcca
    66661 gcgtgttttc ggcagttgca ggaagatatc gacatccagt ccggtctggt ggctggcgtg
    66721 accgccgttg aaacgaccac cagcgggcat ccccatatcg ccaatcagca ccgtacccat
    66781 gcccagattg ctcacctggc gactcagacg ctggataaac atcaccagat ccgggtgccc
    66841 gaaatagcga cgctgatcgg tacgcatgac ctgataatgc tcggactgta tcggcagcgt
    66901 gtcagcgccg acgatacaac cattggaaaa actgcctatc gattgtgcgc tacccggcac
    66961 aggttgggtt attttttgcc acggcgtcgc tgccaggctg gcgctactgg caagcagggc
    67021 cagcagcgca atcgcggttt tgttcatttt ttaccagcgt ggaatatcag tcttcacatc
    67081 ggcattttgc gcccgttgcc gtaacaggtg atccattaaa acgatcgcta gcatcgcttc
    67141 cgcgatcggc accgcgcgga tcccaacaca aggatcgtga cgacctttgg tgatcatctc
    67201 aacttcttcg ccaaagcggt taatggtgcg ccccggcacg gtaatactgg aggttggctt
    67261 cagcgccata tgggcaatga tttgctgccc gctgctgata ccgccaagaa tgccgcccgc
    67321 atggttgctc tggaatccgt ctttggtgat ttcgtcgcgg ttctggctgc cacgcagcgc
    67381 taccacgtca aaaccatcac caatttctac gcctttcacc gcgttgatgc tcatcagcgc
    67441 atgggcgatg tcggcatcca ggcgatcaaa gaccggctcg ccaagtccgg cggggacgcc
    67501 actggcaaca acggtgactt tcgcgccgat ggagtcgccc tcttttttca gcgcgcgcat
    67561 cagttcatct aacgcgtcga ttttgtccgg gtccgggcag aaaaacggat tttgctcgac
    67621 ctgcgaccag tctttgattt ccagcggaat gtcacccatc tgggtcaggc agccgcggat
    67681 ttcaatacca aatttctcgg cgagatattt tttggcaatc gcccctgccg ccacgcgcat
    67741 ggcggtttct cgggcggaag agcgcccgcc gccgcgataa tcgcgcaggc cgtatttttg
    67801 ttcgtaagtg taatcggcat gacctggacg gaaaacgtcc ttaatcgcac tataatcctg
    67861 agaacgctgg tcggtatttt cgatcaacaa tccgatgctg gtgccggtag taacgccttc
    67921 aaaaacaccg gagagaattt tgacctgatc cggctcacga cgctgggtgg tatagcgcga
    67981 tgtcccaggg cgacgacggt cgaggtcatg ttgcaggtcc gcttccgtca gcggaatgcc
    68041 tggcggaaca ccatcgacga tgcagccgag cgccagcccg tgcgattcgc cgaaggtggt
    68101 tacgcgaaag agttgtccaa ttgtgtttcc agccatcacg gctccgttat tgttgtgttt
    68161 gcgtgtttac ttaatcttta taaatcgcga aatgttcacg ggccgccagc agctgctctt
    68221 tggtgagcat aaacacacca tcgccgccgt tatcaaactc cagccaggtg aacggaacat
    68281 ccggatattg ttccataaga tgtaccatgc tgttgccgac ttcacaaatc agcacgccat
    68341 catcagcgag gtaatctgcc gcgtttccga gaatgcgacg cgtcagtttc aggccgtcgg
    68401 tgccagatgc caggcccagt tccggctcgt ggcggtattc gtttggcagg tcggacatat
    68461 cttccgcatc gacatacggc gggttagtga caatcaggtc gtactgcact ttcggtaaat
    68521 cacggaacaa gtcggagcga atcggaatga cgttgtggat cagaccgtgt tcttcgatat
    68581 tctgttcggc aaccgccagc gcgtcgggag agatatcaac cgcgtcgact tctgcatccg
    68641 ggaaggcata agcacaggca atggcgatgc agccgctacc ggtacacata tctaaaatgt
    68701 gctgcggttg cttgctgatg agtccggcaa atttattgtt gatcagttca ccaatcggcg
    68761 agcgcggcac cagcacgcgt tcatcgacgt aaaattcatg accgcagaac caggctttgt
    68821 tggtcaggta agccaccgga atgcgttcgt tgacgcggcg gatcacgcgc tcaacaatac
    68881 ggtgtttttc gctggaggtc agacgcgcgg tgcgcatgtc ttccgggata tccagcggca
    68941 ggtagagtga aggcaacacc agttgcacgg cttcatccca cgggttatca gtgccgtggc
    69001 cgtaccagat attcgccgcg ctgaagcggc tcaccgacca gcgcaacatg tcctgaatgg
    69061 tttgcagctc atttactgct tcatcaacga aaattttatc cacgtattcc tccagggcat
    69121 gatcgcaata atttcggcgg ctagtttgcc atgaagatga cgataaatca gcattcacgc
    69181 gcggtgagtg aggaaaaata cgttttaagc gatcgattgt acggtgagtc gggtaaactg
    69241 taggaaaatt aaaaatagag acagataaat gaaaaagaaa acaacactta gcgaggagga
    69301 ccaggcgctg tttcgccagt tgatggcggg gactcgcaag attaagcagg acacgattgt
    69361 ccaccgaccg cagcgtaaaa aaatcagcga agtgccggtc aagcggttga ttcaggagca
    69421 ggccgatgcc agccattatt tctccgatga gtttcaacca ttattaaata ccgaaggtcc
    69481 ggtgaaatat gttcgcccgg atgtcagcca ttttgaagcg aagaaactgc ggcgcggtga
    69541 ttattcaccg gagctgtttt tggatttgca cggtttgacg cagctgcaag ccaagcagga
    69601 gttaggcgca ttgattgccg cctgtcgccg cgaacatgtg ttttgcgcct gtgtgatgca
    69661 tggtcatggt aagcatattt tgaagcagca aacgccactg tggctggcgc aacatcccca
    69721 tgtaatggca tttcatcagg caccgaaaga gtacggtggt gatgcggcat tgctggtgtt
    69781 gatagaagtg gatgagtggc tgccgccgga attgccctga catcatgacg ggcatacgct
    69841 ttcactgaca gacgtcgacg gttaagtgta agattatcca aacagaactc aacgcagggc
    69901 actcaatgat gaatatccgc acgcttaagc tcactaatct ggggcggttt gaagaacttg
    69961 aagttcatct ggctccagtg gaggagttca agagcaatgt gaccgttttt attggtaata
    70021 atggtgcagg taaaacatca atattaaaat cgttggcaac cagcctgagt tggttcgttg
    70081 cccgagttcg tactgaaaaa ggtaacggta gccctattcc tgaagacgct attctgaacg
    70141 gtaggagttc ggcgacaatt gaacttcagg tactgaatac gcatccagcg acggaggccg
    70201 ctacgcccta ccgttggttg cttgccagaa cggccagtgg gaaaaaatcg accaccgcct
    70261 ccagcctgca agaggccagc caattggctg cgttttatcg agatcaatac acccagaata
    70321 gcggggcatc cttcccgctt atcgccttct atcccgtaga acgtgtcgtg ctggatgtgc
    70381 cgttgaaaat aaaagaacgt cataattttt tgcaactgga tggctacgat aacgccctga
    70441 atcagggtat tgatttccgc cgtttctttg agtggtttcg caatcgcgaa gatgcagaaa
    70501 atgaatcggg cttaccccaa gacgttctgg ataagctcag taccaggata gatctcgata
    70561 acaccgtctt aaatgcatta acggcaatca tggcctcgtc ccgggatcgc cagttgaccg
    70621 ccgtcagaac ggccattagt cgctttatgc cagggttcag caacttacgc gtcaggcgta
    70681 aacctcgcct gcatatgtcg attgataaaa atggccagac actgaatgtg ctgcaattat
    70741 cgcagggtga aaaatcactg atggcgttag tcggcgatat tgctcgccgc ctggcaatga
    70801 tgaacccgat gttagaaaac ccgctaaacg gcgagggaat tgtattaatt gatgaagtgg
    70861 acatgcacct gcatccaaca tggcagcgta caatcatcca gcgtctgacg acaacattcc
    70921 cacattgcca gtttgtctta acaacccact ctcctttagt gatcagtgat tacaaagatg
    70981 tgctggttta ttctctggat aatggcgaat taacgcagct cccgtctctg tatgggcaag
    71041 atgcgaatac tgtgcttttg aatgtgatgg atacggatat tcgcaatgcg acagtggcag
    71101 aaaaacttaa cgatcttttg gatctgattc agaaaaacga ctttatcaac gctaacgctc
    71161 ttctgaatac gctaagcctg gaacttcctg aaaaccatct tgaactggtg aaagccagaa
    71221 tgcttctgcg caaacaggaa attaaacatg cgcgaaataa ctaaagggca accgccggcc
    71281 agtttgcaac agtgggtgag agcaaaacca cgggataaaa atgccaatca gtggtttcag
    71341 gaactttatg cgcaaaaaaa atgggatatc gttggcgatc tcagccagca gtgcgctcag
    71401 gagcaatttt atctctgtgc ttattgctgc gatcgcgtga caggtacaaa ccgcgatacc
    71461 gttaatgaac atgtcgaagc cagggattta gctcctgctc gcagtctgga attcacgaat
    71521 atcgttgcaa gctgcaaaac aaaaggccag tgtgacgact cgcataagaa ccagccattg
    71581 ctgttaacgc cgcttatgcc ggaatgtgaa accgagttta tcttcaagat cagtggtcga
    71641 attgaaggga cgacgccgag agctatcgat gcgattaagg tactcaacct tggcgatagt
    71701 gaacgtaata atcgggcatt gattgaaaaa cgtaagcagc tttccgacag ccttctgtgg
    71761 gctaatggca ttgacccttc agaggggctt gaggatgacg acctgctgca agcggttatc
    71821 gacgaattac tcacccccgt cgacggttat ctggcacctt tcgctcccgt ggtggcgaat
    71881 attctaaaaa actggatgac cgcatgacat aacggatggc cgatacccga ccatccgtac
    71941 cagagtcact acaattcatt caatacatac tgaaaacgac acgtagaatc aatcggttgt
    72001 cggctactgg tattcacctt caccacaata tcgtgcagtt gtttatccgt tactggatga
    72061 cgtgtagggg aaaccgttac tgtcgattta tcgcaaccgt aagaggtaaa ggttaacagt
    72121 tccgagacca cgcggcagta tgctcctctg ttatcaaaaa cgctgccatc acagagtttg
    72181 ccgtcttgca tcaggtaggt cgaaagcgtg gtggtgatgg tcgagccatt ggactgtaac
    72241 tgcaacgctt tactatggct gacataattc tcattccacg aaatatcaaa tgaagtcacc
    72301 ggaatcgttg ggcatgccgt agcatgatcc gttgggccag tatatattcc atatgcatgt
    72361 tgatctgata cctcataaaa agaggcgatc cacggacagc cataattgtc agattgaacg
    72421 gtaactttaa atgtctcata tgcgcatcga aacagcgtcg tatttatccc agggcaaaac
    72481 gagttatcag tgctactata gtcaacaaga gtcaacgttg gcgcagggcc agaccccgtc
    72541 catgaaacga ccctcgcgtc agtgcctata tacccggtag tattgtatac cccataggca
    72601 acctcgcctg gggttaacac agtgacatgt aaggctcctg tggtgaaacc tgctctcccc
    72661 atcccatagt aaattttcac actatcaaaa gttgcgctgg tgagtttatc cctggcataa
    72721 ctctttggcg tacctacact cattacgagc aatatcaggc tcgcgaaaaa catcatccat
    72781 tttttcatct taaaaatact caaaggttgc cgtcgccgtg gcgctgaaat caccgggcgt
    72841 cacatcccgc ccactttttg tctgtaacca ggcattgaag ttgagagtgt tactgccctg
    72901 attcagcggg aacgtcacgc ccgtcgtgtc gttgataggc accgctgcgc ccgccgccgt
    72961 ttcaatacca atcccgaccc cggacgctga cgatgaggcg tctatagcca gaaatccggg
    73021 cagttcgctg tcttccgttc ccgccagcgt cactctgacg ctaaacagcg tagagctttt
    73081 gcaatccttc aactgaaaga ccagcggcac tcgatctgac tgcccaagat gcattaaatc
    73141 gctggcggca atggtaggaa attgcacctc cgccagcaga ctaccgttga tcaccggtgt
    73201 acaggattta ccgagcagat tgccggtgaa gtgcaggttg tcgaccgccg gagcagtact
    73261 gctgcacagt aacagcagac tgctgataaa aaccaacgca tgttttttcg tcattggtaa
    73321 tccacctgta acgtagccca ggcttcaaag ttggcctcag gcaaatcgac gccgctcttt
    73381 ttgaccggta ccgccgttaa cgccggcaaa gccgtttcat ccaccaccag tggcgtattc
    73441 agcttaaagc tctgccctgc ctgtttaagc cgaatcccca ggcctgcgat gtccgtgctt
    73501 accgccgcat cgtcaaagtc gctctgcgtg ccgctccagc tcaaggtcat ttccagtaaa
    73561 gggtcacgaa ccggtgtatc gcaagtaatg cgatacggca cgatttgctc ataattactg
    73621 ccgttaatgt cgtcgatatt cacgtcctga aaggttaatt caatggtttc accgtcgcta
    73681 atggtgcagt taggcggctg cactaaataa ccgtgaaaag tgacatttga gagcgccatg
    73741 gagcaaaagc cccacagcat aagaagtgag atccgtttca ttggtaatcc accacaatgg
    73801 tggcgctggc gctaaattcc gccgccgtta acgacgtgcc gctctgtttc accggaaccg
    73861 cttccagcgc tggtgcgccc gaggagaaat taaacggcag ccatgcgcca gacgttaagt
    73921 cgagtatcga actatcgctg gccttctgaa tccgaatacc aaacccagga atccccgtgc
    73981 tgatcaccgt ttcaccgtta atcacgaccg ttgccgcctg catctgtaga cgcaggtcat
    74041 ccatggccag gttgttacag acaagcttgt aatcaatggg gcgcttataa ttcacgccac
    74101 caattctggt aatgacaaca ttaccgaact ccacctccgc gcccgttacc gtacaaggag
    74161 gcggagcatc aacaagcacc cgtaaaatca tatccaccga tttgctccct gccgtcacgg
    74221 agcaactacc gatgcagccc accagcaacc acgcccacga tcctttcatc ctggctctcc
    74281 cgttatcagc ctttgctgct ttgatcttca tccaccttgc aggtgtcccc ttcacagcgg
    74341 aagatcagcg gcaggcgcgc gccgtagtcg ttgatgtagg tcagcaccgg ggcgctgtcc
    74401 atttttgcct ttaacggctg tgacgttttc ggcgacatca ccagcggttt aaacccggct
    74461 gcggtcggcc ctttcttctg tgtgctggca tcgctgatga tgacgtggta tggcgtcggg
    74521 ttattgacgg tatagtcctg gccgctgcgc gtgagcgtca ccttaaactg ccacggtttt
    74581 ctcatgtcaa tcttctccag cgcctttggt cgccagaaca gcttgatgcg cgtttgcagg
    74641 gcaatttgca gggtgttggc tttcccggtc tttggcggga tctcacgcac gttgtagtag
    74701 aacaggcttt cacggtcaga tggcagcgtg tgaatgtccg gcagcgcctg aatcttcacc
    74761 tggccgttca tcatggcgtc aatacgctgc accggcggca gtaccgccag cggcgaggtg
    74821 attttgttgc ccttctcgtc ttccagccag ctttgcgcca ggtaaggcag cttttcgttg
    74881 ttgttacgca acgtcacgct gatcgattta tcgctttcgt taaacaccag acgggtgcgg
    74941 tccggggtga ccgaggcgtg cgccgccagg ctgacggtgg aaagcagtgc cgccagcgcc
    75001 gtgctgacga ccattgaact ggataattta taagacattg atcgttcctt aatcagatta
    75061 ttgagtagct gaaattgacg tcggcgcttc cgttggtgtg acttgccgcg tctggtcctg
    75121 aataacaggc ttaatttctg gcgctgaaga atcaggggct gccgttccct tttgttcaca
    75181 cggcaacaac aggccgctga acagatcggc aggtagcggc ttcggcaata cgatctcgca
    75241 ttgtgcgctg ccttcccaga acaccgtcat atgctcaccc gcattgactc ccgccagata
    75301 gacattgcct tcatcatcca caatgccaac ctgctgctgc tcgtcgtttt tcacttccgc
    75361 gccaaacggc gggtagctgc cgtcacgcaa ccgcagtacc gccatcgctt tttgcccgct
    75421 gatcactttg aatttgcgat agccaattgc accttccgtc agggttgcct gtaccaccga
    75481 ctgggtggct tccgcatctt ctggcaggtt gtttaaatcg atatacgcct gattgcgata
    75541 gtagttgtta atatcggcga ccaccgcctt gccaaacata ttggtgtaca ccggcgcgcc
    75601 gttgctttcg accggcacat tggcaatccc gtcggcgtca atcagcaggc gcgtaccgcc
    75661 catgctttga gtgcgatgca gcgccccacc atgcgcggtc agcgttgccc cgccctgtaa
    75721 ggcgatcccc gccgagcggt attccccctc atggtagttg gcgctgagat caaccttcgc
    75781 cagcgagccg tcgtgactca gataaccatc cacgctgccg tgctgttcgc tggtaccgac
    75841 gttaacctgg tagtgcgttg cgtcatcaac gcgcttaaag taaccgacct ggctgctgtc
    75901 cgacccgctg ccgtaggaac cgttgtaggt cacggtgctg ctgtcgctcc acggaatgct
    75961 catgctgagg tacatcccct tatccgcgtt atcgtcatat tcgtagcgat agccggtcac
    76021 cgagatgctc acgttgcgaa tgctccccat attaaaatag tgggaaaaca tcaggttata
    76081 gtttgtctgt tccgggcgat cccagtaagt acgatgggag tagttgagat agatcgagac
    76141 acccgcagcg gcaaagttct ggttataggt gatcgtatac atctctttgt cgttaccggt
    76201 ccgcgccatg tccgactggt tcgcgtccag atactcgctc atggtcatga agttcttttc
    76261 agaaaaacgg tagcctgcaa aggtgacgcg gctgttgagt tcgtcaaagt ctttggcata
    76321 gctcacgcga aaggagttgc cgtccagttt tcctttgccg tatgcgctgt catgatccag
    76381 gttgacgtgc gagtgagtca catcgaacgc cagcgcgcca aactgtgcga ggtcgcgccc
    76441 cacccccatc gccgccgact ggtagtgttc atctgccagc gcgccaccgt agagcgacca
    76501 gccatctgcc accccccatg acgcttctcc gccggagaaa aagccgcctt cggtcttgtg
    76561 gttccagtct tccggacgcc ccatcatcac tttgtaacgc acctgcccct ggcgcgtgag
    76621 gaatggcata gatgcggtcg taacgtcata ttcctgcacc tgaccattct gttcttcaac
    76681 gcggacgtgc agtgtgccgg agacggagtc gccgatatcc tgaatgcgga atggcccggc
    76741 gggaacctgg gtttcgtaaa gtacccggcc catctggcta atggtgactt ttgcactgct
    76801 gtgcgccacg ccggagacat ccggcgcata gccgcgcagg ttgggcggca gcatctggtc
    76861 atcggtgctg acgctgctgc caatatagtt aaagccgtcg aaaatatcgg aattgagata
    76921 atcttccccc agcgacagct tcgcttttaa agagggtaag gcccgccagg cgtaataacg
    76981 gctccagtcc cagtttttgc tcgttgtact gttactgctg tcatcgtcgt catcgttgct
    77041 gcgggtgtgc tgataatcac tttgccagtc tgcgcggaaa cgccatgccc ccaggttcgc
    77101 cccaacggta ccgttgccgc tgatatcatg tgagtcctcg ccaccctgct cctggtggcg
    77161 ggtttgcgca ttgaggctgt agtcggcaat caggccagga ataccgtcgt cccagcgtga
    77221 gggtgggtcc cagtcgctgc tggtatattc gagataagcc tgtggcactg tcagcagcaa
    77281 cgccgactgg cttaaatcat tctcgacttc catcccatct aattgccccg gtttaaggca
    77341 ttcatcgttg tgcgtccact gcaggctttt tgctatccct tctttcagcc ccagcgccgc
    77401 cactaactca ggtgtcaggc aggcatacgt ttttgttgga tcgttttcag aaacatacca
    77461 gttaatgtcg tattcatcag aaaggggttg tttatttata aatacacgta aattatattt
    77521 tcccgcgtcg acataccctt tttttgaaaa cttaccgaga tcaattttcg tgtcgccctt
    77581 taactcaagg aaacgggggt caaattgaat ggaatcatca gcccatgcat tgactgatcc
    77641 acctgagatt gccagcgcaa tatagcaggc gattccccgc agccgaaaat ttgagtgatt
    77701 aggcatacac atatccgttt gtgaaacagt cgtaaatctg ttgcgtgttg aaaaacccca
    77761 cagcagaacg ctgtggggtt gtcacatcag agataggtaa tctggaatgt ggtcagggtt
    77821 tcgaactggc cgagatccgg cgcttcggtt tcacccacca gccaggcttt aaagttcagc
    77881 gtttgcttgt ctttaccttt gctggttgaa gcatccatca cgattggctg aacaatcttt
    77941 tcgcccactt tataaccttt gccatgggta tcgccgatca ccaggctcac attcttcatg
    78001 gcaacaccgc tgtcaacgtt gaataacgca tagttatctg tgttggctgg ccccgcgtag
    78061 ttagtaccgg tgaacgtggt ctccatgttt gtttcagtgg aaaacacgca gtcctgtaaa
    78121 cggatctgga aatctttcgg tgtggtggtg ccaccgccgg tcagtttagc cgcaccgatg
    78181 tcacccatat cgacttccag tttgtgatcg tctggtacga tagagcacgg ggagtcttca
    78241 atggtgccgt agaagcgcgc ggtaccgttg ttgcctgctg caaatgcaga actggaaaca
    78301 actgcgccca tgaccatcgc cgcagcaatg gctgttttgg caaacttact cattatttgt
    78361 ccttaaatca ttaagagtta taatataaac acctgacata aaattaatat atattatgcc
    78421 cgatatttat agttagccat tgaacatata cactccaata taatcataga atgtaaacaa
    78481 gtattctctg gtgtgtctgt atgttggtct tatctataaa aaaactcaat ttagtaatac
    78541 ttaaacactt tatgtagatt ttattaatta atacatctac aaaatacttt caccactttc
    78601 caacaaaaaa acatgcgaat tatcaacaaa aaaacaaact attataatca acaaaaatga
    78661 attgcttata aagcacacta aaaaaaatta taatgacctt accatcattt attgctaaaa
    78721 tttaatatta attaaatcaa taaatatata ttcaagacac aaaaccatta tcttctttat
    78781 tttattacgg gattcaataa tgggaaaata taataactat cgagagttgt taaatattaa
    78841 caacatttag acaagaaaaa gaatgattgt atgacggaga aactgcccgg tgtacgcgca
    78901 cgcaaccggg cattgattgc ttaaattgct tttgccatct tcaaattaca agggctcatt
    78961 tgccagttga attggccttt tccgctttca tcaagcgtta cgctggcaat cgctgacgtg
    79021 gtaaacatag gcggcgtttc gcccggacat aactcggcaa ccagataccc gactaagggt
    79081 aagtgggaga tcaccagtac cgaagcaaca ccttcattgg tcaacgcctg caaataggca
    79141 ctgaccagac caacatcgcc gcagggtgtt aactccggca gaacttctgc actggaaggc
    79201 aggttcagac aatcccctac ttcttcaagt gtttgctcgg ctcgcaggaa cgggctcacc
    79261 agaacgcgtt cgatttccac tttttgacct ttcagccagt tcgccatcag gcgagattcg
    79321 tcacaaccgt tagtagtcag aggacgaacc gaatcactgg cggcatcgag ggctgcgtcg
    79381 ccgtgacgca tgataaaaac ttgcatattg caccgctttt gttaaccaga ttcgcccgtt
    79441 ttctttcatc tgaaaccaga gaaagaaaac ggtggccggc attgtgcctt atccattcac
    79501 cgaatgaaac gctgtttttt acctcaatgg cgtaagtata gtcaatatgc gtttacattt
    79561 tgagcaacac tccgccattc agcgcggatt catatagctt tgacctgctt attgcaggtc
    79621 agttgcagtt gttttccaaa aactttcccc acgcttgctc atctcgacta aacgctcgca
    79681 aggggtaaaa cgggaaccat actgcgtggc cagtcgttgc attattgcaa ccacttcgcc
    79741 tgcgccgaga gaatcgatat agcggaacgg tccaccgaga aatggcggaa aaccaatgcc
    79801 aaataccgcg ccaatatccc cgtcacgtac gctacggata acctgctcgt ccagacaacg
    79861 tactgcttca ttcagcatca acatcacaca ccgttcagca acctgcggtg cggagagtcg
    79921 cccctgccct tgtgcgccaa tgagcgggta aatggcggga tcgacctgtt ttttgctttt
    79981 acgccctttc tgaccataaa gatagaaacc ccgaccattt tttctgcctt tgcgatcgtc
    80041 gttcaaaatt gaagaaacaa catttgcagg cgcgctaaaa cgttctccat aagcggcttc
    80101 cagtacagga ataattttag tcccggtgtc gattcctacc tcatccaaaa gttggattgg
    80161 gcctaccgga aaaccaaatt tcaccagcgc ggcatcgata tgctcaatac gctcgccttc
    80221 ggtcaacatg cggatagctt cattgatgta aggcgctaag atgcgattga cgtaaaaccc
    80281 ggctttgtca cgcacgacaa ttggcgtttt gccctgtttt ttcgccagtt ttaccgtggt
    80341 ggcgatggtt tgcgccgatg tactcgcatg aggaataatc tccaccagcg gcattttttc
    80401 caccggactg aagaaatgca ggccaataac ttgctcaggt cgcgcggcgt gagcggcgat
    80461 atcgccaatc ggtaaagatg acgtgttcga agcaaagatg gtatgagtag cgcaattttg
    80521 ctccacttcc gccaccatct gttgttttaa ttcgagattt tcaaacaccg cttcaataat
    80581 cagatcgcga tgggcaaagc cgcaatagtc cgtcgttccg gagattaaag ccagctgttt
    80641 gtcacgttcg ctggctttga gatgacgacg gcgaactttg ccctccagct gatcccaact
    80701 gtacttcagc gcatgattta tgccctgcgg gttgatatct ttaattctga ccgaaagccc
    80761 cgctttacaa gcagtgacat aagcaatacc gccgcccatc aagccaccac cgagaatccc
    80821 cacgctgttt aatggcgcag gcggcgcatc actgccggaa tctttcttca cttccgtact
    80881 ggcaaaaaag atattgcgca gcgcctgcga ttgcggcgtc atcgccagtt cgccaaacgc
    80941 ccgtgcttca gcgtcatagc cgctactggt gccctgcgct aatcccgttt cgacaacctc
    81001 caggatgcgt tctgtcgccg gatagttacc ctgagttttg tgttctgttt tcttgccgac
    81061 cattttgaac agcagcgcac gacctaacgg ccccgccaga atacgctcgc gtacaggtag
    81121 agggcgtgaa gatggacgat cctgctttgc cagctcaaca gcggcttcca gtaaaatgga
    81181 ttgcggaacg acgtcatcca ccagccccag ctttaatgcc tgtttcgccc gaagttgttt
    81241 tccagtgagg atcatctcca atgctgtgct gacacctatc aggcgcggta aacgctgggt
    81301 gccgcctgaa ccgggtaata atccaagttg tacttcaggc aaaccgagca ccgttttagc
    81361 atcatcagta cacacgcgac cgtggcacgc cagcgccaac tccagaccac cgcccaggca
    81421 agcgccatga atagccgcga taaccggaac gggcaaagca tgaatctccg ccattaactg
    81481 ttgcccctgc cgcgccagta cttccgcttc ttgcgccgtt ttgcagttgc cgatcatgtt
    81541 gatgtctgcg ccagcaatga agttgtctgg tttcgcggag acaaacacca cgccacgcaa
    81601 ctctttgttt tcacggagtt gcttaataat ggcgcgtacc tgcgaggcaa attccgcctt
    81661 cagggtattc attttctcac ccggtacgtc gatggtgatg atagcaatgt tgtccagacg
    81721 aacattaaga gtaaacgctg atgccatttc cattattccg cctccacaac cattgccgcg
    81781 ccaagcccac ccgcagcaca ggcggtcact aaaccaaatc caccgccgcg acggcgaagt
    81841 tcatgcaatg tctgggtaat catccgcgcg ccggtcgccg cgaagggatg cccgtaagca
    81901 atcgaaccgc caagcacgtt aaatttgcta tcgtccactt cgccagtggc atgtgcacgc
    81961 cccagtacat cacgagcaaa acgttcgcta cccagcaact gaatattcgc cagtgtctga
    82021 gctgcaaagg cttcgtgcat atcgatcaag gtcagatcgc tcatcgtcaa tccggcccgt
    82081 tccagcgcca gcggcgttga ccaggccgga ccgagcaaca tgtcctgcca gacatctatc
    82141 gcagtaaatg cgtaactgcg cagatacccc agcggtacca gccctaattc tttcgcccgc
    82201 gattcagtca tcaggatcac cgctgccgcg ccatcggtca gcggtgtact gtttgctgcc
    82261 gttaccgtcc cgtgtttgcg atcaaacgcc ggacgcagct ttgcgtaatc agcaagcgtg
    82321 gaattaccgc gaatattgtt gtcttcggca agcggttgtt tataaggagg gataaaggca
    82381 gtcatcacct cttctttgag tttaccgtct gaccacgcct gagcggctcg ctgatgcgaa
    82441 cggtgcgcta atgcatcttg ctgttctcgg gtgatgccgt aggttttcgc catttgctct
    82501 gcggtgtccc ccatccgcaa gccggtagaa tattctgcta ccgcaggcgg tacgggcatt
    82561 aagtcacgca aacgtaagcg agaaaaaagt ttcaggcgct ggctcattgt gcgggctttg
    82621 ttgacatcaa ccagcacgcg cgccagtttt ttgctaacgc caatgggcaa tacggaagag
    82681 gaatcggccc caccggcaat ccccgcgcga atagttcccg ccatcagact ttctgcgacg
    82741 tttgcaatcg cctggaaact ggtagcgcaa gcgcggctga cgctgtaagc atcggtatgg
    82801 acattcattc cggtgccaag aacaatttca cgcgcaatgt tgggggcttc cggcatctga
    82861 acgacctgac caaagaccag ttgttcaatc acttcggcgg gaatttcgct gcgtgctagt
    82921 agttcgccta ccaccatctt ccctaaatca accgcgggaa tgccatgaaa agccgtcgcc
    82981 tggcgggcaa aaggcgtacg taaaccgcta acaatggcga tacgatcgcc caggcgggta
    83041 accagcggta aaacctgacc cataacactc ccctgtaaaa taataaataa agtggtctga
    83101 cctgatcata gtcttaacca tttttttaca tttagccaag tggagaaaag ggaaagtggg
    83161 agctatgaca cagagagaaa agtagaagag aaaataaaac gcccccgcca ggaactgaca
    83221 ggggcgtata tacccgagag gaattaacgc agacccagct ggaagatcag catttcagct
    83281 tcgcaggcaa aagtgaaatc gatatccagg cgcacaccgt cagactcttc agtgaaagtc
    83341 ggggtgattt tgcacggttc ggattccacg ctacgggctt tttcagtcag cgccgccagc
    83401 gtttgttctg cttctgcgcg gtttgcaaac acgcggctat aagacgcggt gcagtcggag
    83461 ttgtccataa tggtgccaac atccatacag cagcaaaccg gggtttcatc agcactacat
    83521 ttactcatcg ttgatttcct ctgtatgtgc acccaaggtg ccagataaac gttgtggata
    83581 ttttacgctt ccggaaagtg ctgctccagt tgttaattct gcaaaatcgg ataagtgacc
    83641 gaaatcacac ttaaaaatga tctaaaacaa aattcacccg aatccatgag tgcgccacct
    83701 ccaaattttg ccagctggat cgcgtttctt agatcatatt tgaaaaaaga tagaaacata
    83761 cttgcaacat tccagctggt ccgacctata ctctcgccac tggtctgatt tctaagatgt
    83821 acctcagacc ctacacttcg cgctcctgtt acagcacgta acatagtttg tataaaaata
    83881 aatcattgag gttatggtca tgagccagaa aaccctgttt acaaagtctg ctctcgcagt
    83941 cgcagtggca cttatctcca cccaggcctg gtcggcaggc tttcagttaa acgaattttc
    84001 ttcctctggc ctgggccggg cttattcagg ggaaggcgca attgccgatg atgcaggtaa
    84061 cgttagccgt aaccccgcat tgattaccat gtttgaccgc ccgacatttt ctgcgggtgc
    84121 ggtttatatt gatccggatg taaatatcag cggaacgtct ccatctggcc gtagcctgaa
    84181 agccgataac atcgcaccta cggcatgggt tccgaacatg cactttgttg caccgattaa
    84241 cgaccaattt ggttggggcg cttctattac ctctaactat ggcctggcaa cagagtttaa
    84301 cgatacttat gcaggcggct ctgtcggggg tacaaccgac cttgaaacca tgaacctgaa
    84361 cttaagcggt gcgtatcgct taaataatgc atggagcttt ggtcttggtt tcaacgccgt
    84421 ctacgctcgc gcgaaaattg aacgtttcgc aggcgatctg gggcagctgg ttgctggtca
    84481 gattatgcaa tctcctgcca ggcagactcc tcaggggcaa gcattagcag ctaccgccaa
    84541 cggcatcgac agtaatacca aaatcgctca cctgaacggt aaccagtggg gctttggctg
    84601 gaacgccggg atcctgtacg aactggataa aaacaaccgc tacgcattga cctaccgctc
    84661 tgaagtgaaa attgacttca agggtaacta cagcagcgat cttaacccgg cttttaataa
    84721 ctacggtttg ccaattccta ccgcgacagg tggcgcaacg cagtcgggtt atctgacgct
    84781 gaacctgcct gaaatgtggg aagtgtcggg ttataaccgt gttgatccgc agtgggcaat
    84841 tcactatagc ctggcttaca ccagctggag tcagttccag cagctgaaag cgacctcaac
    84901 gagtggcgac acgctgttcc agaaacatga aggctttaaa gatgcttacc gcatcgcgtt
    84961 gggaaccact tattactacg atgataactg gaccttccgt accggtatcg cctttgatga
    85021 cagcccagtt ccggcacaga atcgttctat ctccattccg gaccaggacc gtttctggct
    85081 gagtgcaggt acgacttacg catttaataa agatgcttca gtcgatgttg gtgtttctta
    85141 tatgcacggt cagagcgtga aaattaacga aggcccatac cagttcgagt ctgaaggtaa
    85201 agcctggctg ttcggtacta actttaacta cgcgttctga taacgcgttc gcctggataa
    85261 agtcacctgc tatgcaggtg actttaactc ccacacttca acgaaagttg tttcctcatt
    85321 gcttgcctcc tgagttttgc aaacagcctg atggtagctt gccctttcaa atcattaagt
    85381 ggtatccaca gacaaacctg aagggaaagg catattttca ggcgttctgc tcgtctttct
    85441 caaagagttt acttttctga atttccagga tactcaaacc accctggcta ttgtgcgctc
    85501 atacactcaa attaaagata gggtctaaat aaatgagcgt tttttgatag tctattttat
    85561 taggtaatat atatttgtaa caaatcaatc aacatggaat aaaatcatgc taccatctat
    85621 ttcaatcaac aataccagcg cagcttacac agaatccatc aatgaaaata acaatgatga
    85681 agttaatata ttagtacaag agctcaaaaa cctttttaat ggtaagcaag gaataagcac
    85741 ctgtgttaaa catctacttg cgcttataaa aaacgccata cgaggaaacg acaatcctga
    85801 tagatttaat attaataatt cctcagttac ttatattgat attggctcca atgatacaga
    85861 ctatattact attggtatcg ataaccaaga aacaataaaa ttacttgcga gctataaaga
    85921 caaagaactc gtccgtgcta tcattaatga caacatagtt gagaagactc atgatgatat
    85981 aaataatgct gactttactg atggtagtat cgttgttagt ggtaatgagg gtgctattga
    86041 ctccaatgat acagccgata ttactagtgg tatctataat caagaaccaa taaaatcacc
    86101 tacgaacgat gaaaacaaaa aagtcggcca aaccatcatc catgaaaatg tagtagagaa
    86161 tactcatgat atcaataaga aggaaatgat cttcagcgca ttaaaagaaa tatatgatgg
    86221 agatcctggt tttatcttcg ataagatatc acaaaaactc agacatacga taacggaatt
    86281 tgatgagaat gggaaaagcg aaccaacgga cttatttacc tggtacggta aagataaaaa
    86341 aggcgattct ctcgctattg taattaaaaa taaaaacgga aatgattact tatctctcgg
    86401 ttactatgat caggacgact accacattca aagaggaatt cgtattaatg gtgatagtct
    86461 cacccaatat tgtagtgaaa aagccaggaa tgcttcagcg tggtttgaaa gcagtaaagc
    86521 tatcatggca gaatcatttg caacaggttc cgatcatcag gttgtaaacg agctcaacgg
    86581 ggaaagactg agagagccaa acgaagtttt taaacgttta ggtcgagcaa taagatataa
    86641 ttttcaagtg gacgatgcaa aatttagacg cgataatgta aaagaaataa tttctacttt
    86701 agtcgataac gaagttgacc gttctcaaaa taaatatgac cactttaagg aaatcgaaga
    86761 taaagttgaa aaaaggcttc aaaatcgcca ggctaaatat caaaatgaaa ttaatcaact
    86821 atctgcattg gatgttaatt ttgatgataa ttaaaattgt ccagtttttt tgatttttca
    86881 acgtctgaat atcggaaaca gacaatgacg aacatcagta tttttacata attatgtcca
    86941 ggtcattgtc ggtttatctt cttttacttg gggtaattga gggataacaa caaaaaaggt
    87001 gagttttgtg actcaccttt tttatttgtt tcttattcag aatcgatatc ttttaaatca
    87061 tcctgaatcg cttgcgcgtt cggattttcc tgcggtttga gttcgccgcc attagcgatg
    87121 aaatcatgac gctggaagta cgcttcgcgc accataatat aaggatcgga cgactgacgc
    87181 agcagaccat cggaatccaa caactgcgca cgagtttcta tcccttcaag cgtccattta
    87241 cccacagaca tcggccaggt cagccaggaa agaaccgggt aaagaccatc cgccatatca
    87301 ccaccgtcat cacgcagcgt gaagctaccg tagaacggta actgaacgta tggcccataa
    87361 cccacaccat aatgaccaag cgtactaccg aagcggtgag gttcagtccg ttgcagtttc
    87421 gggttcgcca tccctgcaac atcaataaag ccgcccatcc ccaaaatggt gttcaggaaa
    87481 aagcgggtaa agtggaccat cccctgataa gggtcgccct gcaagaagta gttaaccatc
    87541 accgcaggtt cttcaaggtt gccagtaaag ttgctcaaac cgttacgcgc cggttgcgga
    87601 acataatcac gccaggcgac agcgaccggt cgaacaatat acgggtctaa tacattgaag
    87661 ttgaagttgt acatggtgcg gttgaaccct tctaacgggt cagaacgccc ttgctgatct
    87721 gtaccggaac tcgcacaccc caccagaagc gtagttccca gagcaagcgc cgacaggcga
    87781 agcttcataa atgtctccct gtttttttat ggcttatgca gtttgccatc catgacggaa
    87841 cgataccgta tccgcctgtt taggtgtggg cgattgtaac agcacgtcaa ctgatgtcca
    87901 gacgccctga tttgctgatt tgatcatagc ctggtaatcg ccgccctgta ggctacttga
    87961 ttctatagaa acagaaaaag gcaaacgcgg ccttttctac attttcagag taactcccgc
    88021 cagttgcgag caaaaaagcc gctacgcttt agctatacgt gctaattcaa gagaagagac
    88081 catggacaac gacaaaattg atcaacacag cgacgaaatt gaagttgaga gtgaagaaaa
    88141 agagcgcggc aaaaaaatag aaatagatga agatcgactc ccctcccggg cgatggcaat
    88201 tcatgagcat atccgccagg atggtgaaaa agagctggaa cgcgacgcaa tggcactact
    88261 gtggtcagcc attgcggcgg gtctgtcgat gggcgcttca ttactggcaa aagggatatt
    88321 tcatgtcgaa ctggaaggtg tgccgggcag cttcttgctg gagaatctcg gttatacctt
    88381 tggttttatt atcgtcatta tggcccgcca gcaattattt accgaaaata ccgtgactgc
    88441 ggtactacct gtcatgcaaa aaccgacaat gagcaacgtt ggcttactta tgcggttatg
    88501 gggcgtcgtg ctgctgggta atattctcgg gacaggtatt gcggcgtggg catttgaata
    88561 tatgccaatc ttcaatgaag aaactcgcga tgcatttgtc aaaatcggca tggatgtgat
    88621 gaagaacacc cccagcgaga tgtttgccaa cgcgatcatt tccggctggc tgatcgccac
    88681 tatggtatgg atgtttcctg cagcgggtgc ggcaaagatt gtggtgatta tattgatgac
    88741 ctggcttatt gccctgggtg acaccactca catcgttgtc ggttctgttg aaatcctcta
    88801 tctggtgttt aacggtacgc tgcactggag cgatttcatc tggcccttcg cactacctac
    88861 tttagcgggg aacatctgcg gcggcacctt tatcttcgcg ttaatgagtc atgcacagat
    88921 tcgtaacgac atgagcaaca agcgcaaagc agaagcacgc caaaaagcag aacgtgcgga
    88981 aaacattaag aaaaatgata aaaacccggc ataaatggcg agggtttaag caatcgagcg
    89041 gcagtgtact taccccgcag tccattagcg ggtatactca tgccgcattg tcctcttagt
    89101 taaatggata taacgagccc ctcctaaggg ctaattgcag gttcgattcc tgcaggggac
    89161 accatttatc atccccctcg caataaccca cttacaatca gtgagttatc ttaaaatcct
    89221 tagtatagtc aaccagcact tcgatatgtg aacgcaaatc atcatgcatt cgtgaactta
    89281 actggaaacc tcccccattc gatgtcaaaa tgactacccc aataagttgc caataatcag
    89341 gctaatcaat acgggtcagt cttgctggtc agtgatcata aaaatgtcac ctcatattag
    89401 acacagcaaa taattgtgtc gattaaacat actaccttca gcataaccgt tcctgaaaaa
    89461 ttattatatt cagtaatctg aataaattac tggactgcta tttttctcag caaccatatt
    89521 tagcagcaag ccagaaaatc tgaatgatta cagatgctgg cctgctgaat ctcccttctg
    89581 gaaatcgtca gaatctagtg attacttact ttgttctggt ttctcttcat tcttattgtg
    89641 cacttccact ttgggtaatt tattaaaaac cagattttct ttttcaacca gagtcatgac
    89701 ttctttggtg atatcagctg actcgttatg cgcaagcaca acacctgaag acaatacggc
    89761 aatattttta ggattgtctt ttctccatgt gcctatagca ttatctatcg cacgggataa
    89821 caccattttt gcgctatttt cctcaataac atattgtcgt tccagttttt gtctggcatc
    89881 aagaagcagc gcctctttat tcgcgttatc tgcgaatact ttttgcgcat cctcatatcc
    89941 tttttcaaga atactcttaa cctcattcag atgttttctg gccatgtctc cggcggcaga
    90001 ctgattataa atagcatcga tattaacaac tgcaatttct tttgactttt tcatataaaa
    90061 aacaccagta agtacaatca gcaacagcag cactactgca ttaacaacag aaaatttcat
    90121 tacagaactc attatgacaa ccctgaggtg gcaggaggtc ccctcctgcc actgtgtgat
    90181 tagaatatat atttaatacc cagcattgcc tgggcatcac tgtacccttt atcgccagcc
    90241 tgaacaccaa cattgcccca gacattcagg ttatctgaca atttaccctg aacacccgtt
    90301 ttgacttcag caatatttgt agctccgtcc tgactgagtg ccgtattatc cattttgaca
    90361 ccccagctcc gtgtattatg gatccagtta acttccacaa atggctcgaa ctgatgcgcc
    90421 ttattatcat cagacgcact cttacctttc aggtaagttc tgacgcccag acgactctgg
    90481 atattaccgt ctccgctgag ctgaactttc gtgccattct tctccgtatg ctcactggct
    90541 cttacattca tccacgtgat ctgggactgc ggctgaatat accagtcata gtgactaccc
    90601 tggctaccgg taaattcgcc ggctttaaag gtataacccg cttccactga accggtaaat
    90661 ccacgggatt tccaggactc cgcagccagt ttttcaccat taacggtatt attaaaccat
    90721 ccgtactgga tccaggtatc gacataagcc ccgttgtcat taccgtcatt ctgctgccac
    90781 gttccgtata aaccggtgct gtatccattg atcgcacctt tagatttata tcccgaaagc
    90841 gaggatgtcg tggaactctt ctcatttcca taaccggcca taatccccag ttgcagacgg
    90901 tcctgaccgt cagtccattg cgcaatgctg ccacccagtt gcacaacata acggttactt
    90961 tgcgtattca gctgtgaact gctgtccttc cagcgattat gaccgccgat atgacgcatc
    91021 cacatacttg ttgctttctt ctcaccagta aacacatcgg tatagtaagt ttccccctca
    91081 cgatcgtgca ttctgatgtt gaacagcgtg ttggctgccg ctatgttcgc aatataagag
    91141 cccgcttccg gacgatagac cttctgtaca ggtttatccg gcgtggggtg tggctgtggc
    91201 tgcggttgtg gctgcggctg cggttgtggt tctggcggca attcagaacg cagataccag
    91261 ttaccgtcag taccacccgc attaccttta tgcaggaaat attcccatgc ccctgccacc
    91321 gcacggttct gcagtctgaa gttaccgtca gactgcccac cgacatgcac aatctcaatg
    91381 ccgtcagcgg tctgagcccc ctggccccgg acattattca ccaccacgcc ggtatcaccg
    91441 gaggtgttac ccgtgacaat cagtttgtca gtggcagaag tatcatcccc cagcacggta
    91501 ttgataatta acgtaccgcc accagtgtaa tcgccattta cggtcagggt atttcctgtc
    91561 ttaccaccgg ccagacgaat gacgccggta ttggtcagtg agccaacggt aaaattgccc
    91621 gcctctgccg tttcataacc tgagagagca ttcagtgatg ccagtgttcc ctggttatta
    91681 acctgacccg aaacggtccc cagcccactc agtgtgccgc cattaagaac atgaatgttt
    91741 ttggatcctg acaacgtcgc gccagcggca cgggtgactg aatcatcacc gacaatcaga
    91801 acgccattct caaccgtggt atcaccggta taagtcagct catgattaat gatcagatta
    91861 cccgccccct gtttcaccag agcgccggag ccagtaatat cattcttcag taaccagttt
    91921 ccactggcgg taagccaggt gacaccatca ctgtcaatca gcccggtgcc caggtgtttt
    91981 tcatctgaag cccgcagagc agcatctttc tgcacacgga attcaccgct gaaattacgg
    92041 ttatctccgg aaagtgtcag atcagtggtg ttcagtatgc ccactgaacc ttttccggaa
    92101 agcgatttac tcaggttact gctgactccg ctaccgtcgg cgatatcaat cgccaggcga
    92161 tcattaaggc tttcaaagct gatggagccc tggcttccca gcccctgtgc gccgttcatt
    92221 tcaaccagcg aaccccgggt aaggctgaca ttaccggtga acccgatatt ggtgccatgt
    92281 accgtcagat tgctgcccgt gacagccagc gtaccgtctc cggtcagcac attgttttca
    92341 atgatgccac cattatcatc gcccggctga cgctgcgtgc cgctgaccgt gagtacacta
    92401 ccgctatcaa gctgaatgtg cgctcccgtt tctgtctgca gtgcacccac cgtctggctg
    92461 tatccgtctg ttctgaagac ggcattgctg gcaacgttca ggctacctgt agcgccaagc
    92521 acattatcat ttgccatctg cagtgtgccg ctgcgcacca gcgtatcccc ggtgtagtta
    92581 ttaccgccgt tagacagtgt gacaacctgc cccgcagctt cgatggccag atcccctgcc
    92641 cccgttaact gtgcaccaag atctgtctgc agtccctggg cattcgctct cggtgtcagc
    92701 accagcgctt tatcaccggt tccctgtaaa tccagcgact tcagcccata gcctatgtac
    92761 agtccgtcac ctttaacacc gtcggagctt cccagcagct tatagtcata atttccctga
    92821 gctacaactt caccgccctg agtgacatca aacgtctgac tgtgagaaat agcctgcccg
    92881 ttttcatcca ccagttgcag ttgcccgcca gtaccggtga cggtacccgc tgcattcacc
    92941 agtgtgacca gggtctgcgc atcatcctgc tccagcaaat tcttacgggt atcaacagcc
    93001 ggaatatcat taatcacttc atctgcatgg tgacctgaat tgtgcctttc ccccggatat
    93061 ccagcgtacc tgcagcagag gtttcaatac tgttgctggc aatggtatcg cccggcataa
    93121 cggaaccaaa aatcagcgtt ccgccattga acccaagacc gccaatctgc tgaacaccgg
    93181 agccaaccgt tgtgatattc ccggtttcag acatcagcat tgcatgggtt aacccgctcg
    93241 tattcagccc ttccagagca aagctactgg tacccagctt cagagttccg gcaaattgat
    93301 ccccgacgtt atggctgaat tcaaaagcag atgtgtcggc atcaagacgg gcagacagtg
    93361 ttcctttgcc tgttaacagt ggatcaaagg taaagttgcc tgcaactgcg ggaataatgg
    93421 ccagttcgct gcccgattca atatcgacgg aacccgggcc ggtcccccca tcggtcagca
    93481 caagagcttc cttcagagtg acaagcgaac tgttcttcag cccgacatgt tcaaaattcc
    93541 gtagttttac agcagaatcc agcgtatgaa tggcgttatc aaaaatcagg gaatcagaat
    93601 ctccctgccc gccatccagc aggttccagg ttgccccttc gcctttaatg gtgaaggtgt
    93661 tcttcccggt accggcagta accttatcaa catgagcctt accattgagc gttacattgt
    93721 tattgccatt tcctgcagcc acttctcctg tcagtatgct gccatcatca aataaaaatg
    93781 tattgttacc ctcttttgca ctgacggttc cggtaatact gcctttattg gtgaatgtgt
    93841 tatcaccgcc gttcagagcc acaaccccct gaatttcagc accgctgtca ttcagtacgg
    93901 tggtattcac ggcgtcatca aacgccattg cagtcccggt ggcggacgca gctttaatct
    93961 gacctttatt ggtaaaggac tgtgcttttg aagcatccac tacggcatga ctcagagaag
    94021 cagagatgag attaccgctc tgaaccactt cactggccgc attgttaacc accagagcgg
    94081 aaccgccatc ggcctgtgta acattgacac ttgcaccact cttcacgaca gcaccatctt
    94141 tagtgttggc gaaaataccc gtgccgcccg tgcctttcag attaatgact aatccgccgg
    94201 agtcagacat atccaggtta ttatcggttt cacttccgtc agctttctgg aacgccagtg
    94261 caattccact gccgtctaca ttaatagtgc cgctgttggt cttggccaga gaagcagaag
    94321 ttcgcacacc aatgccatca gccacattaa tttcagtcgt attggttaac tgaatgcctt
    94381 caatttctgc acggttctca atcccgtgac cgaccgcacc tgcagcatta acattgattt
    94441 tcgcaccatc aataaccagc cccacagccc ctgtatccag caggatgcca tgcgcatttt
    94501 tctgaccttc gatcgttccc aggccactcc ctgccaggtt cagtgacgcg cccgccccca
    94561 gcttaatggc agcctcacca tccacagcga cgatatcccc tcccgtgctg gtaatctgag
    94621 cgtgttcacc ttcaacaaat aatgcaacgc cattgaccgc tatacggctt cctgtatttt
    94681 caaaacgacc attatcaacc cagacgccga tattatctgt accggtagtg aaatcaatgt
    94741 tgccgctgtt aatcaacagt ccctgattac gcgtaataaa gcccttggca ttattcagcg
    94801 gcgaagagat atctgcctca ttggtaataa ccgagccagt attcgtttgt gtaatactgc
    94861 catccagagc gtattcattt ccgtcaactt ccgcgacaac agcgccatcc cctacaaggt
    94921 taactgatgc cccctgtttc agcgtcgctg tcgcaccacc agttacatat aatccctgag
    94981 cctgagcgcc caggatctgg aaatcactgc cagcatcagc caggacattg ctccctttcc
    95041 ctgtggccca gataccacgg ctgcctgtcc cggaggtttt catcagcaaa gagccggcct
    95101 gttttgcccc atcttcaata cggaaaagta cagaacgttc cccactggca tccagcagag
    95161 ttcccggtgc aatattcgtc ttaatggttg caccatctcc gataatacga aaagcaatct
    95221 gatcggtaat accagagccg tcagaagcaa agttcggtac tgccgagcca tcaaggttaa
    95281 cagtcccttt attgctggcc tcaacgccaa tcgccccctg tccctgcaaa gatatagtac
    95341 ccgcggtcat attaaccgtt gttgttgtac cagatgcttt tatacccgtt gtacggttag
    95401 cattatctcc ccccttgaga acgagatttc ccgagttatt aatcgttgta acatcttgtg
    95461 tggcattagc aattagtccc acaccaccat cctgaactgt aatatttccg ctgttggtac
    95521 caacagcacc ctcctcaacc cggacgccca ctgtattctt tccagtaaag atgatattac
    95581 cggtattatt tagagtggcc ccattacggg cgatataccc ggtcacttta tcctgggtgg
    95641 aacttaactg agcccccgcc gttaatagtg tggtcttgtc cttcggatta atgagtttgc
    95701 cggaaatatc atagccattg ccatccgcaa taccggctat tgcactggca ttatccatat
    95761 tgataatggc attactttca attgtgccct gcgctccccc ttcaattaaa gtcgctgtag
    95821 ccccctcccc cgtcaggttg atggtcattc ctccagacgt tactgtcgag gccacaccgc
    95881 catccgattt ccccgtggca ataagtgcat aagagttctt accagacgcc gtaagtgcag
    95941 aagaagcatc tgcagttccc tggaatgtcg caccgctggc aatacggaat aatgttgagt
    96001 tttcagtgga cacatccata acaccgcttc cggtattatg aatggcagag tcctttccat
    96061 agacataaaa gcctatctga tcgctgctgc caaatagcac tgcaccattg cctgacaagg
    96121 taagactgcc gccatctttg gcaaaaaccc ccacagcacc agtaccggaa agattgattt
    96181 ttccggatac attaacattg cttctggcac cttccaccca ggcaccatag ttacggaaac
    96241 cactactgat accctcgccg ccaacattta tcgtaccgtc agaatttaat tgccctgcat
    96301 tgattacctg tagcccggta ctgttcagac cattaacatt gataatcccg gagtttttca
    96361 tcccggagct attatttgcc agcatgccaa cattcagtgc aggtgcatca ctgtcataac
    96421 cgtcaatagt gatagtccct ttatttacca catcaacact cccggcatcc tttgccagga
    96481 ttccgtagtt cccctgagcc cccctgccca aaaaaatagt accttcatta acgactttgt
    96541 ttgcaccatt ttgtacttca atcgcaacag aacccacagc cgaatgagtc gcagttttat
    96601 cctccgcaat acctacattg atgctcccga aggccgagtt aataaaagag cctccagctc
    96661 caacatttac acctcgtgtt ttgccattat tatcattagt tgatgcatcg gcagtaatat
    96721 ttattgtgcc tttatttgtt gccatgcccc cattactaac aacaattgcg gtgttaatcc
    96781 cctctggagc gaaagcattt ttaaatgggc gaatattaat aacaccatta ttaatggctg
    96841 aagaaccatt actaccggca accatcccct ctgcctggag agcatattta tcattattat
    96901 cccaaacaaa aagtgtacca tcgttaacaa tggtactgtc ttgataggca atcatttcag
    96961 taccaatggc atcaatgacc ccttcattaa caccggtccc accattagtt gcaacgatcc
    97021 ctcctccatt cccataggca ggaagaactc cagttaattt acccgtttta cctaatattg
    97081 ctcgtgcgtt attggttgca tggagaacag caagcagccc tacattttct ttgtaaggag
    97141 ttgagtctat acgtccccca gcggtcatat ccacattgta attatgcttg gtgacaataa
    97201 gggccttatt aaattcagca tcatactgct tataagaaag cgttttgtcc tctagtgact
    97261 ttatcaaata ttgattatat tgctttaaat catcaagaga gttaatatgg tgtacttttc
    97321 ccgcccaatc agtaattgtt ccagcatatt tcattgagtc aatgattttt tgagtaacgc
    97381 cgcccccctg catggttgca ccaggctgaa aataaaaatc attgaccgag ttccatacaa
    97441 cagaggaacc ttctccatca gctacagcaa gctgtgtatt tttaaaaaaa ttaccgattg
    97501 ctggtgaacc cacatccaaa atagctgtac cgccattatt ggctgttaca aaacgtgtcg
    97561 cattgaaaaa atctgcagtg ccactaaaac caggaatggt ttcatttcct ccagtccccg
    97621 catcgcgttg tgagaaagag tcagtatcaa acacttgaag tgtttgataa gcgccagtaa
    97681 cctcatcctt cactgtaatg gtttttgttt tttctccggt attgacgtaa atcaatccgg
    97741 ataaattatc tccagtggtg taaccattta aaattgcctg ttctacagtc atccagataa
    97801 gacctgaccc accagactgg acgacatttg gaccagtgat gtattcggtt ttaccactta
    97861 ctgttgcagc cccaacaccc gagttattcg tacccggacg ccagtctggt gacaatatga
    97921 catctgccat tgcgttattg caattaattg caatagcgcc ccccagaacc attgctgata
    97981 aagcaattcc taagcgagtt gatttacatt tttttctaca actcgtcaat tctgaaacaa
    98041 catcccattt ttgggttgta tggttccaaa tgactttata tatcttattc ataacactaa
    98101 tactcttgaa cgtacattat aattgaatat tttcactaat aagggaacgc ggaatattta
    98161 taaattgatg taaacgtttt accccgagtt tatccatagc tctacgacga tgcgtccaca
    98221 cagtcttact ggatataccc aaggtgttag agatccatgc aatatctttt ccttccttgg
    98281 tatatataaa gatacttttc tccctcgggg ataattgtcg ttctaaaaaa gaaactttat
    98341 gactcgcgcg aatatttttt ttgcacctcc agccattgat aaatttatct atatagaaac
    98401 aaaattgctc tgctgttaat ttccacggta tggaaggtat gcctttatta ctaactacac
    98461 cattatcccc ccatgccagg cacggaatat tgacaggctg gctttttagc cattggtgaa
    98521 tgaaatacag tgagcgtata caggtagtag aatcattatc agggaataca ggtaacaaca
    98581 acgattgctc atcccgaaag caagatgtgc caaaatcacg aatcagagaa gaatagtcgt
    98641 ccacagcgat gactttcacg tacgaaaaac cccgccaacg catactctta attgcttgaa
    98701 ttataaaaat attttttgtc gtcgtaatca aaaccatgca atcaatttcc tgttatcccc
    98761 tttcccaatt tccttttgct ttgcacaccg gggggagaca aaccttgagg cacaggttta
    98821 caaaatgacc cattttgaca acatcaattt aattaactat aaatagcaca aaacgcaggt
    98881 aaaaaacctg cgtatttatt ttaattcaga atataatttt gattattatt tacattttgg
    98941 ctttatacgc caaagattaa aaagaagaca aacctacctc tttatgtgta gattgacgtc
    99001 acacattgat ctaaatcagt aaatttaatg ttttttatag aaaaaaatgt ttttctatac
    99061 gagaatttct aaacaaagat tgggtttaca ttttgggtca aaatgttgct tttttgcgtc
    99121 acctctcccc cacatttttt caaatctcat tgaattagaa gcagtataaa ttaccgtatg
    99181 cctgatatta cgatgcccga gatagtcctg aataagacgg gtatccacac cattatctgc
    99241 tagtgaataa ccacatgcat ggcgtaacat atgtggatga actttaatat ttaaacctgc
    99301 atttttaccc gcagcagaaa ctatgttata aaatcgttgt ctcgataatg gttttcctgt
    99361 tcgggaaaca aataaccacg ggttatcatt gaaatgttta acatttaatg aattcctttc
    99421 attcagccag tttgttattg caatcacctc ttccttttga agtggatgga ctgtactgaa
    99481 tccatttttt atccgttgaa tatatatttt ttctgtagtt agttctaaat ccgacaactg
    99541 cagagataag agctcactaa cacgcagacc atgaaaataa gccaataaaa tcatgcaaac
    99601 atcgcgtgaa gagcaactct tttgtcttac agattgaagt agtaaattaa tttcatgatg
    99661 cgttaaaaat tttctgtttt gcataaatca cctctattta tataaaataa ccatgtcaac
    99721 tatgatgaaa gaattacatt tagtcaataa tataattcat atagttgctg tatgattata
    99781 gattaattta ttaaaattca cctaatctaa ttaatcgctt gtaaacgcat ataaacaacc
    99841 ttcgttatgt aactatcgtt caaaaccctc ctgtaaatat aaagagccct ataagatgat
    99901 tataaaaaag tcctacaacc cgaaatagta ttccctcata aaattaagct caacatcggc
    99961 atatttaaaa agtatccaat gcaattaata ttttatctaa aattaacatt gtatcttgta
   100021 tatattacat ccatgaatgt atgaattttt ttatttaaaa attaatgcac tataacaaaa
   100081 aacatcaccg agtgttaaaa ctaacacaac aattaaccaa aataatatga ctgtttattt
   100141 ttcatccgta aacaaaaagg tacaaatatt ttcaccacaa aaaaaccacc agcatgtcat
   100201 tgaattacta ttcgcaatac acacctcctt gtgcttattt atgctcattg gttgactaat
   100261 aatgatctat gcccgctggg caactatctc tctggcgggg tattgatgaa tatccattta
   100321 gtggcataat ttaaacccca gctcattatt tgcttataaa aaacaagcaa taataccagg
   100381 ctctggtatt tatagtttat tttttaactt aattagtata accgataata ttatgcgcaa
   100441 atttattact catagtgaat ggttgttatt ttttgaagcc atcaacggtt caaaaaatga
   100501 aattagagat aaagccatgt tacaaatggc atatgtacat ggtcttaggg taagcgagct
   100561 tattgcacta aagattagcg acattgactt ttcagagtca gcaatatata tcaagcgttt
   100621 aaaaaatgga ttatccacag tacatcccct gcaaaaagaa actgttctgt tattaaaaaa
   100681 atggctagca ctacgtgata acatagtaaa aaaacctttt gaagactctt tatttctttc
   100741 ttgtcaagga aataaaatct cgagacaata tgtgtataag atgtgtaaga aatatagtca
   100801 caatatgaat attaatattc atcctcacat gcttaggcat ggttgtgggt atgctttagc
   100861 taatcaaggg ttagacacca gactaataca agattattta gggcacagaa atatacatca
   100921 tacggtatta tatacagcca gtaatgcagc aagatttaaa cgagtatggg agggtgatgt
   100981 gttagatata aagaagatat aacccctgtt ctaaaggcca caattcattg aggcttttag
   101041 cctctcattt ttatgacata caactgataa tcataatgga tcctcaatat taatcaaaat
   101101 gagaaataaa tcaccttttt attaaaaatt aattttttta aagtcacaaa ccaacacata
   101161 taatatttaa taaaaatgcc accagcaaat atattgccac ttatcttttc tctggtaatc
   101221 tagtttacta taaattatcg ctctccaata ttcgacgcca agaagatgtg gttattttat
   101281 ttatcctttt cgtaactatt gacaaaacaa ttcccatata aaaattgcaa tttataaagc
   101341 caacatacac ttacagtgca taataaggtt atttaggaac cagatgattt aatgtattat
   101401 aaggtataac attcatattg acttacctgt tctcttaacc aagtaataaa tgcctcaatt
   101461 tttggccact gcctgcccgg taatgtggtg atgtaataat gctgatggca tttcaccgtc
   101521 atatcgccaa acggcgcgac gagctcacca ctggcaagcc ttttttgtac caggcgtttt
   101581 cttcccatcg ctaccccaat atgattcatc gcagcgataa cagctaaatc agaacgatca
   101641 aagccaatcc cagaagatgt cggcaaatta accgcataat gttgcgccca actatgccat
   101701 tcatccgtac cggagtcgtt gctccatgcc tgtctgtcat ggagcaacgt acagtggcgc
   101761 aggttaatta cctggttggt taaagcatgt ctttgagcgt attccgggct acatacaggc
   101821 aagatttctt catccatcag aaagtgatga gccaattgcg ctgacggcgc atcatcaaag
   101881 tagatcgcca aatcgattcc ggcacgttgc aaattgacat tgtcattacc agtgagcacg
   101941 gtgagcgaaa tagacggata tcggcgtgta aagtcaccta atgcgggcac caaccagcat
   102001 tgggcgatag agggccggga atacagcgtt aacgttcccg ataattcctg atttttgata
   102061 tcaagaattt cctggttcag ggtatccagc gacgatttta gcgcccaata aacgcgtttc
   102121 ccctcgtgcg ttaattccac tttgcgatgg gaacgaacaa acaactgaat acctaattct
   102181 tcttccagct gattgatacg gtgacttacc gcactggggc tcagcgacaa ttcctctgcc
   102241 gcaagggcga aggactgatg cctggcagcc acttcaaaag tatgcagttt tgatagttgc
   102301 cagccgttaa gcagtcgatt tcttatttca cgaaggggtt ccataatcac ctcatttttc
   102361 tcttaagtgt aaaaaaatag cggcaaaatt tcagctgtaa ggtgagctaa agtgaaccat
   102421 atctcaattc accttcattt ttagatgtaa atcactccct tgatgcaatt tagctcatgt
   102481 gaaaggcaaa ttttatcgtt tgtcagcctg cgttgttttt ttgtccaata tcatcaggtt
   102541 aatcacaggg gaaggtgaga tatgcactct caaatctggg ttgtgagcac gctgcttatc
   102601 agcatcgtgt taattgtact gaccatcgtg aagttcaaat tccacccgtt tctggcgctg
   102661 ttgctggcca gcttcttcgt gggaacgatg atgggtatgg gaccactgga tatggtaaat
   102721 gctattgaaa gtggaattgg cggaacgctg gggttccttg ctgcggttat cggcctgggc
   102781 acgatactgg gaaaaatgat ggaagtatcc ggggccgcag aaagaattgg tctgacactt
   102841 caacgctgtc gctggctttc agctgatgtc attatggtgc tggttggcct gatttgcggc
   102901 atcacgctat ttgttgaagt gggcgtcgtg ctattgattc ctctggcttt ttcaattgcc
   102961 aaaaaaacca atacctcatt gttaaagctg gccattccgc tatgtaccgc attgatggca
   103021 gtgcactgcg tggttccccc acatccggct gctttatatg ttgccaataa gctgggcgca
   103081 gatatcggtt cggtgatcgt ctacggtttg ctggttgggc tgatggcatc actgatcggt
   103141 ggcccacttt tccttaaatt tctgggtcaa cgactgccct ttaaacctgt acccacagag
   103201 tttgccgatc tcaaagttcg cgatgaaaaa acactaccgt cattaggcgc aacgttattc
   103261 accatactgc tacccattgc gctgatgttg gttaaaacga ttgccgaatt gaatatggcg
   103321 cgtgagagtg gtttgtatac cttgcttgag tttattggca accctattac tgccatgttt
   103381 atcgccgtgt ttgtcgccta ttatgtgttg ggtatacgcc agcatatgag catggggaca
   103441 atgctcacac atacggaaaa tggcttcggt tctattgcta atattttgct gattatcggg
   103501 gccggaggcg cattcaacgc cattttaaaa agcagcagtc tcgctgatac gctggcagtt
   103561 attctctcca atatgcatat gcacccgatt cttctggcct ggttggtggc acttattctg
   103621 catgctgcag tgggctccgc tacggtggca atgatggggg caacggcaat tgttgcaccc
   103681 atgctgccgc tgtatcccga catcagcccg gaaattattg cgattgctat cggttcaggt
   103741 gcgattggct gcacgatcgt tacggactcg cttttctggc tggtgaagca atattgcggc
   103801 gctacgctca atgaaacatt taaatactat acgaccgcga catttatcgc ttcggtcgtc
   103861 gctctggcgg gcacattcct gctgtcattt atcatctaag cgcaaagaga cgtactatgg
   103921 aaaacgctaa aatgaattcg ctcatcgccc agtatccgtt ggtagaggat ctggttgctc
   103981 ttcaagaaac cacctggttt aatcctggca cgacctcatt ggctgaaggt ttaccttatg
   104041 ttggcctgac cgaacaggat gttcaggacg cccatgcgcg cttatcccgt tttgcaccct
   104101 atctggcaaa agcatttcct gaaactgctg ctactggggg gattattgaa tcagaactgg
   104161 ttgccattcc ggctatgcaa aaacggctgg aaaaggaata tcaacaaccg atcagcgggc
   104221 aactgctact gaaaaaagat agccatttgc ccatttccgg ctccataaaa gcacgcggcg
   104281 ggatttatga agtcctggca catgcagaaa aactggctct ggaagcgggg ttgctgacgc
   104341 ttgaagatga ctacagcaaa ctgctttccc cggagtttaa acagttcttt agtcaataca
   104401 gcattgctgt gggctcaacc ggaaatctgg ggttatcaat cggcataatg agcgcccgca
   104461 ttggctttaa ggtgacagtg catatgtctg ctgatgcccg gacgtggaaa aaagcgaaac
   104521 tgcgcagcca tggcgttaca gtcgtggaat atgagcaaga ttatggtgtt gctgtcgagg
   104581 aaggacgtaa agcagcgcag tctgacccga actgtttctt tattgatgac gagaattccc
   104641 gcacgttgtt ccttgggtat tccgtcgctg gccagcgtct taaagcgcaa tttgcccagc
   104701 aaggtcgtat cgtcgatgct gataaccctc tgtttgtcta tctgccgtgt ggtgttggtg
   104761 gtggtcctgg tggcgtcgca ttcggactta aactggcgtt tggcgatcat gttcactgct
   104821 tttttgccga accaacgcac tccccttgta tgttgttagg cgtccataca ggattacacg
   104881 atcagatttc tgttcaggat attggtatcg acaaccttac cgcagcggat ggccttgcag
   104941 ttggtcgcgc atcaggcttt gtcgggcggg cgatggagcg tctgctggat ggcttctata
   105001 cccttagcga tcaaaccatg tatgacatgc ttggctggct ggcgcaggaa gaaggtattc
   105061 gtcttgaacc ttcggcactg gcgggtatgg ctgggcctca gcgcgtgtgc gcatcagtaa
   105121 gttaccaaca gatgcacggt ttcagcgcag aacaactgca taatgccact catctggtgt
   105181 gggcgacggg aggtggaatg gtgccggaag aagagatgga gcaatatctg gcaaaaggcc
   105241 gttcataacg tttcaacgca gcatcgccat cctttccctg ggtgagcgat gctgccgatg
   105301 gcgcagactt aagatccccg gtcttacccg ctataacccc ttgcttttac catcacccaa
   105361 cgcctttcgc tgtaaacggc ggtttcgcaa accaaaccaa aaccgtaagt aagataaaag
   105421 caatagccgc catacggaaa atctcatttg cagaaataga aagtgactgc tgggttattt
   105481 cattattaat ttcattaaga actcctgaaa gcgaaccata gtatttatcc ataatttgtg
   105541 acgatgaatt aaacacgggg ttaaattgat cgatggttgc tgtcaactga ctatggtgta
   105601 atgattctcg gcgtccccac agcgtcattg tcaacgacgt accaactgat cctgacaagg
   105661 tgcgaaaaaa attactcata ctcgaggcat tggcaaattt attatctggc aagcctgaaa
   105721 acgaaatcgt tgttaagggt aaaaagaaac aggcaacggc gaatccctga aaaaactgtg
   105781 gcaaaatgat gcctgtaaaa tcaatcgttg gcataaatgt cacagaacgc cagtaatagc
   105841 aaaccgcata catcaaaaaa ctaaatgtca ccaacaaccg catatctatt ttgttgccat
   105901 aacgtcctat caaaggtgat attaatagcg gcatgatacc gatgggcgca taagcaagcc
   105961 cggcccatat cgcattatac cccatcgttt cctggagtaa ctgcggcata aggacgatcg
   106021 ctccagagta aaataaatac gcgcatgtga tactcacaat accaatggtg aaattacggg
   106081 acttaaacaa actgagatca agaatcgggt tctctgaggt cgactcccaa atgactaaag
   106141 agatcagaga aataactgat actactgtta gtattattat tgtactcgag ttgaaccaat
   106201 ccagatcgcg ccctttatca agcataattt gcaagccacc aacaccgagc actaacaggg
   106261 tcagtcctgg tagattcatt ttgaccggtg aagtctcggt ttctcttcct ttaagtaagg
   106321 ttaagcataa tgtcaggacg ataatcccca tagggacatt gattaaaaat atccaacccc
   106381 agctaaagtt atcacaaata taaccgccca atatcggccc acatatcggc gcgataatca
   106441 cggtcataga ccataatgcc agagcaaatg ttcttttttc tggtggataa ttccttaata
   106501 ataaactctg tgacagtgga attaacggcc ccgccattaa tccctgaacg actctaaaaa
   106561 atatcagcac atcaagattg gtcgataaac tacacattaa ggaagacagc gaaaaaaaag
   106621 tgactgaaag taaaaataac cttaattcgc ctattctttg tgccagcctg ccggtaacag
   106681 ggatcgcaat ggcatttgct acgccaaacg aagtgataac ccaggtgcct tcgtctgttg
   106741 atgctcccag aaagccagat attgtcggta ttgcgacgtt agaaatagtg gaatccaaca
   106801 tttgcataaa cgtcgctaat gacaatgcaa tagtaacgca ccataacgtc ccaccggtta
   106861 atggtgccgg agttgattta gtgattgcca ttaaagttgt ccattatgcg aaataatgtt
   106921 gctaatttct ttttctatcg gactggtatc gataattaaa gccttactgg tataagcagg
   106981 catggacgtc acggttgaag ccagatcggg catatcggca atgtcttcgt tcctggtatc
   107041 aatagttgca gtcatcgata aaccaatgcg caagggatgt tccatgagtt cttttggatc
   107101 aagagaaacc tcaaccggta cacgctgaac gattttgatc caattccctg tcgcattttg
   107161 tgcaggtaat aaggagaacg cattgccggt tcccatattg atccctgtca cccgaccatg
   107221 aaacacaaca ttttcaccat aaagatcgct aataatattg accgattgac caatccgcac
   107281 atccgtgagt tgtgtttctt taaagttggc attaacccac atttgacgtg ccggtacgac
   107341 agccattaac gattgtccgg gactcactgt ttcgccgacc tgaacacttc gctgggcaat
   107401 atagccggta accggactct taatatccgt acgtttaagc gccaaccagg cttctttggt
   107461 tgcatccgcc gcttcaacga cttgtggctg acggtttaat ggtgtgttca ttactaaagc
   107521 tttattcgct ttataagcct gaatagcagc attcaatgcc gctttgctac ttattaacgt
   107581 atctttggta tgctccagcg tttcttttga aataaccccc tgcttcgcta aaggtacacg
   107641 acggttataa tcttctaaag attgttgata ctgaatacga gctgaagcga cttcggcact
   107701 gtattgttta tcctgtaagt acagtttatt cgtttgccga acaatattgg ccagattatt
   107761 tttagcttta ttgagtgcga tagtggcatc agtcttatcc agtgagacta aaatgtcacc
   107821 ctgtcgaacg tagttcgtat ctttatgatt aacgacagtg acgctgcctg aaacttgtgc
   107881 agaaattgga tctgcatttc ccgtgacata ggcgtcatct gtactaatca tgtcttctaa
   107941 ttccattgac caataggcat aggcacctga aaacgcaata aataaaacta ccgctaacaa
   108001 agcaaaatat ttttttctgt cagaatgttt tttatttgaa ttaatctgtt ccacttttat
   108061 ctctcatttc gcatagatga aatttatgaa ttgaatgaaa agagtattca ttcttcatat
   108121 caaacacagt gcatgtgttg cacattatcc caggaacgtt ggaggcctaa tttaaatgag
   108181 gtgcaaaaaa aacaacagag gactaaaccg tggcttttgc aatacaattc ttacgcctgt
   108241 aggattagtg agaagactta tagtgctaat gtgaaactat aaatcatcgg tacaatccct
   108301 gattttattg ttgacatttc atatatgccg actatttata tggtatactt gtcgaattat
   108361 cttaaaggaa gctcaaattt tcttattttt attgagaaaa tgagatgatg ccttatgtct
   108421 gtattactac agggagaagg gagatgcttc attgcaaagg gaataatcta tgaacgcaat
   108481 aattattgat gaccatcctc ttgctatcgc agcaattcgt aatttattga tcaaaaacga
   108541 tattgaaatc ttagcagagt tgactgaagg cggaagtgcc gttcagcggg tggaaacact
   108601 taagcctgat atcgtcatca ttgatgtcga tatccccgga gttaacggta tccaggtgtt
   108661 agaaacgctg agaaagcgcc aatatagcgg aattattatt attgtctccg ctaaaaatga
   108721 ccatttttac gggaaacatt gtgctgatgc tggcgctaat ggtttcgtga gtaaaaaaga
   108781 aggcatgaac aatatcattg cggctattga agctgcaaaa aatggctact gctatttccc
   108841 cttctctctc aaccggtttg ttggaagttt aacgtccgac cagcaaaaac tcgactcctt
   108901 atcgaaacaa gaaattagtg tcatgcggta tattcttgat ggcaaggata ataatgacat
   108961 tgctgaaaaa atgttcatca gcaacaaaac tgtcagcacg tataaaagtc gcttgatgga
   109021 aaaattagaa tgcaaatcac tgatggatct ttacacattc gcacaacgta acaaaatcgg
   109081 ctaaccacat gaagttttta ccctatattt ttcttctctg ttgtggtctt tggtcgacca
   109141 taagtttcgc agacggagat tacatcgaat atcgtggcat cagtagtaac aaccgtgtca
   109201 cacttgatcc actacgtctg agcaacaagg aattacgttg gctagcgagc aaaaaaaatc
   109261 ttgtgattgc agtacacaag tcccaaacag ctacgttgtt gcataccgat tcgcagcaac
   109321 ggattcgtgg tattaatgct gattatttaa atcttttaaa aagagcgtta aatatcaaat
   109381 taacactccg ggaatacgca gatcatcaaa aagcaatgga cgcgctggaa gacggtgaag
   109441 ttgatatagt gttatcacat ttagttgctt cgccgcctct taatgatgac attgctgcaa
   109501 ctaatcctct gataattacc tttccggcgc tggtcactac ccttcacgat tcaatgcgac
   109561 cgcttacctc atcaaaacca gtaaatattg ctcgagtagc aaattatccc ccagacgagg
   109621 taattcatca atcatttcca aaagcaacaa ttatctcttt tacaaattta tatcaggcat
   109681 tagcatccgt ctcagccgga cagaatgatt actttattgg tagtaacatc attaccagca
   109741 gtatgatttc ccgttatttt actcactcct taaatgtagt gaagtattat aactcaccgc
   109801 gtcaatataa ctttttatta accagaaaag attcaatcgt tcttaatgaa gtactcaatc
   109861 gatttgttga tgctttaaca aatgaagttc gctatgaagt atcacaaaat tggcttgata
   109921 caggaaacct ggcctttctg aacaaaccat tagaactcac tgaacatgaa aaacagtgga
   109981 ttaagcagca tcccgattta aaggtgctgg aaaatcctta ctcgccaccc tattctatga
   110041 cagatgaaac tggctcagtt cggggcgtga tgggtgatat tcttaatatt attaccttgc
   110101 aaacaggttt aaatttttct ccgatcaccg tttcacacaa tattcatgcg gggacacagc
   110161 ttaaccccgg tggatgggat atattgcccg ccgctattta tagtgaagat cgagaaaata
   110221 atgtttcatt tgctgaagcc ttcataacaa cgccttacgt ttttgtcatg caaaaagcgc
   110281 ctgacagtga acaaacatta aaaaaaggaa tgaaagttgc cattccatat tattatgagc
   110341 ttcattcgca attaaaagag atgtatccgg aggtagagtg gataaaagtc gataacgcca
   110401 gcgctgcatt tcacaaggtc aaggaaggtg aacttgatgc tctggtcgcg acacagttaa
   110461 attcgcgtta catgatcgac cattactatc ctaatgaact ttatcatttt cttattcccg
   110521 gcgttcagaa tgcatcactt tcgttcgctt ttcctcgcgg agaaccggaa cttaaggata
   110581 ttattaataa agcactgaat gcaattcccc caagcgaagt tctgcgcctg accgaaaaat
   110641 ggattaaaat gcccaatgtg accattgata catgggacct ctatagcgag caattttata
   110701 ttgttacgac attatccgtt ttattagttg gcagtagcct tttatgggga ttctacctgt
   110761 tacgctcagt tcgtcgtcgt aaagttattc agggtgattt agaaaaccaa atatcattcc
   110821 ggaaagcact ctcggactcc ttaccgaatc caacttatgt tgtaaactgg caaggtaatg
   110881 tcattagtca caatagtgca tttgaacatt atttcactgc tgattactat aaaagtgcaa
   110941 tgttaccatt agaaaacagt gaatccccct ttaaagatgt tttttctaat acgcatgaag
   111001 tcacagcaga aacgaaagaa aaccgaacaa tatacacaca ggtatttgaa attgataatg
   111061 gcatcgagaa aagatgcatt aatcactggc ataccttatg taatctgcca gcaagcgaac
   111121 atgctgttta tatttgtggt tggcaagata ttaccgagac gcgtgattta attcatgcac
   111181 tcgaagtaga gagaaataaa gcgatcaatg caactgtcgc aaaaagccag tttctggcaa
   111241 caatgagtca cgaaataaga acaccaataa gctccattat gggcttcctg gaactactgt
   111301 cgggttctgg tcttagcaag gagcaacggg tggaggcgat ttcacttgcc tacgccaccg
   111361 gacaatcact cctcggctta attggtgaaa tccttgatgt cgacaaaatt gaatcgggta
   111421 actatcaact tcaaccacaa tgggtcgata tccctacttt agtccagaac acttgtcact
   111481 ctttcggtgc gattgctgca agcaaatcca tcgcattaag ttgcagcagt acgtttcctg
   111541 aacgctacct ggttaagatc gaccctcagg cgtttaagca ggtcttatct aatttactga
   111601 gtaatgctct caaatttact accgagggag cagtaaaaat tacgacctcc ctgggtcaca
   111661 ttgatgacaa ccacgctgtt atcaaaatga cgattatgga ttctggaagt ggattatcgc
   111721 aggaagaaca acaacaactg tttaaacgct atagccaaac aagtgcaggt cgtcagcaaa
   111781 caggttctgg tttaggctta atgatctgca aagaattaat aaaaaacatg cagggcgatt
   111841 tgtcattaga aagtcatcca ggcataggaa cgatatttac gatcacaatc ccggtagaaa
   111901 ttacccaaca agtggcggct atagagacaa aagcagaaca acctatcaca ctacctgaaa
   111961 agttgagcat attaatcgcg gatgatcatc cgaccaacag gctattactc aaacgccagc
   112021 taaatctatt aggatatgat gttgatgaag ccactgatgg tgtgcaagcg ctacacaaaa
   112081 tcagtatgca acattatgat ctgcttatta ctgacgtaaa tatgccgaat atggatggtt
   112141 ttgagttgac tcgcaaactc cgtgagcaaa attcttcctt acccatctgg gggcttacag
   112201 ccaacgcaca ggctaacgaa cgtgaaaaag ggttaaattg cggcatgaac ttatgtttgt
   112261 tcaaaccgtt gactctggat gtactgaaaa cacatttaag tcagttacac caggttgcgc
   112321 atattgcacc tcagtatcgc caccttgata tcgaggccct gaagaataat acggcgaatg
   112381 atctacaact gatgcaggag attctcatga ctttccagca tgaaacgcat aaagatctac
   112441 ccgctgcgtt tcatgcacta gaagctggcg ataacagaac tttccatcag tgtattcatc
   112501 gcatccacgg tgcggctaac atcctgaatt tgcaaaagtt gattaatatt agccatcagt
   112561 tagaaataac acctgtttca gatgacagta agcctgaaat tcttcagttg ctgaactctg
   112621 taaaagaaca cattgcagag ctggaccagg agattgctgt tttctgtcag aaaaatgact
   112681 aaattgcggc tcccacaatg ttctaatgtg ggagctattt accagcacat cttacattta
   112741 tgatgagaac tcctggcgaa tttgttcccc atgctggtcg agcgttgccg ctcccggcat
   112801 aacatgcggg tccgcgcagc cgctgatttt tatcggatta cctggcatca ttattcctcc
   112861 ggcttcaatc aacatatttc tcgcctgagt ttgtggcaaa ttaatggcct cagccacact
   112921 taataacggc gcgacgggta caccaacttc atgtattctg gctaaccaaa cttcagctgc
   112981 ctgcgttttt aacgttcgct caatatattg tttaagaata gcctggtttt gtacgcgtaa
   113041 aatattgctg ctaaatcggg gatcattaac cagttctgta agctccagtg cctggcataa
   113101 cgcagaaaaa agcttgtcat taccacaaca aattgtaatc ggcttatcct gagtatcaaa
   113161 aacatcaaaa ggtgccatgt agggatggcg atttcccagg cgttgtggtg acttcccagt
   113221 cgcgatatat gccatcagtc catgctccag aaaactcagc gtggcatcaa acatcgctat
   113281 atcgacatgc gcacctctct ggctcttttc gcggccataa agtgcactca ctattccgct
   113341 gaataaataa acaccaccac atagatccgc aagagaggta ccaacgcgca ctggcggagc
   113401 atcagggtat cctgtttcca tcattatccc gctcattgcc tgaatgatgg tatcgtaggc
   113461 aggagcatct tttagcggac cggtatgtcc gaaacctgac gatgaagcat atataaggcg
   113521 cgggttgatt tcttgtaacc tttcccatga aaaccccagt ttttccattg tacctgggcg
   113581 aaaattctca gctaatacat cagcttgttt aagcatattt ataaatatac ttttatcgtg
   113641 atcattcttt aaatcaagaa ccacactctc tttgccatga ttaataaaac tgtaatagag
   113701 tgactgtcca tccacatagg gaccaaatgt gcgggtatca tcaccatgac ctggcggctc
   113761 aactttaatt acccttgcgc ccatattaca aagaagttga gttccgaaag gcccattaag
   113821 gacatgcgtc atatcgataa ctaataagcc ttcaaacggc cctttgcttt cattatttgt
   113881 catttgctta tgccttgaaa aaagccgaag gtgaaaagac acttcggcat ttatgtaaaa
   113941 tcaataacat gattaattat gaaaccagtc gactgacata aatccacaag ggagccgtga
   114001 cgacaaagcc cagtacgctc acagccaatg atgctgtacc agtgcgggta tagacattaa
   114061 accggctggc aataatgatt ccggagaatg ccggcggtaa ggcgcctgcc agtaccatca
   114121 tctgcaaatg ttcgctgttc aaatgacatg ccatacccac gagaagcagc gccagtggca
   114181 tcagaatcag cttcaggaag gtgttataag caatttcggc actgaactcg aatttatgtg
   114241 cagccagagt caaccccgca gcgaataccg ctacccctga gttagccttc gcaatcagat
   114301 taaaggttgg gtcccatgct gccggaattt ttaccccaac caacaccagg atcgttgcca
   114361 gaacaggtgc ccatactact ggctcctttg ccgcagaaat taatgcgctc agattgctat
   114421 tcttcttacc atccgctcct gaggaaggat tcaacaaata cagaccaata ggaatagtaa
   114481 ttgcgttaac aataatagaa ataattgcta ccactaaacc tgttgatacg gaatcaccat
   114541 aaatagggtc gagaactgca aaccccagga atccgatggt aggtgaacct gcaattaatg
   114601 cacatactgc tgcttctgca tgggtacgtt taaaaaattt gtagcaacca aaccaggaga
   114661 aaaagaaaca tccgacaata accacaagtg atacaagggt caggcgagtg tccgcaaaaa
   114721 tcatttccct gtttgcccgg gtaatagaaa caaatagggc cgcaggaaga gcgtagttta
   114781 ataccagttt attaaatgcc cgggcttgat cttctgaaaa agtttctcgt ctgccgctaa
   114841 aataacccaa cagcatgatt acgataatag gcaataaatc gccaataaaa aatgttagca
   114901 taatagtatt tccgataagg gtcccctcac catcgataat gagttggcaa gaggagacga
   114961 tgtcagtatt taattaccag cgacttgttt tgggttaagt ttggtaatat ggccactttc
   115021 agttcctgct gcagggtcga tgaccacatt aataatggtc ggtttgcgcg actgaatacc
   115081 ggtggttaaa gcatgacgaa gttcatctgt cgtggtgacg ttatagccaa cgccacgaaa
   115141 cgcatccatt aatttgtcat accttgcatg gtgcaacaga tctgttggtg atggtgcacc
   115201 agcgccactg agatcaacac cgtctcctct gtagatgccg ccattattaa aaataacgat
   115261 cgtcaccggc aggttatatc gacaaatcgt ttcaatctcc atcccactga aaccaaaagc
   115321 actatcacct tcaatggcga caaccggaga accagaggtc acgctagcac cgatggcata
   115381 gcccataccg atgcccatga caccccaggt gccacaatcc agacgacgac gtggtttata
   115441 catatcaata atatttcgtg cattatccag ggtatttgcc ccttcattaa ctaaataaat
   115501 atcctggttc tcgcgcaaaa catcgcgcac ggcactcaat gcattaaagt aatttaatgg
   115561 ttgggtatct gtacttaact tttcatgcat tttttgtgca ttttgctgct tgtggatatt
   115621 taaaatatcg cgccatacca gtggagtcgt aaatgtgttt tgtttcagtt ctgccagcat
   115681 accttgcata ctggatgcaa tatcgccaac gactggcaca gcaatggggc ggttgctgtc
   115741 aatttcctgc ggttcaatat ccagttgaat aaactgtgta tctgccgccc atcctttttt
   115801 accgtgtgcc agtaaccaat tcagtcgtgc accaacaagc atgacaacgt cagcatttgc
   115861 cagggcaaac gaacgcgcag ttgccgcaga aagtggatgt gtatcttcca ggatcccttt
   115921 cgccatagac attggcagga atggaatctg ggcactttca ataaattcac gaagctgttc
   115981 atcagcttgt gaatacgccg cgcctttgcc aaggataact aatggtcgtt cagcttttgc
   116041 taaaagcgaa attgcgctag cgactgactt cgggcatggc aataatgctg gcgacggatt
   116101 ttcaactttt acaatcgtgg ttaacgcttc gtctttttcc atcgtcgcgg ccaggacatt
   116161 tgctggcaaa tcaagataaa ctccgccagg gcgacccgat actgagaccc ggatagcgcg
   116221 tgccaatgca atgccaagat cctgcggctg attaacgcgg aatgctgctt tggcatacgg
   116281 ttttgccgca ttcatttggt ccagctcttc ataatcacct tgctgtaggt cgacgatcgc
   116341 gcggtcgctg gagccgctaa tcatgatcat cggaaaacca tttaccgttg cgttggccaa
   116401 tgcggtcaaa ccattgagga atcctggcgc agacactgtc aggcagatcc ctggtttttg
   116461 ggtaagaaaa ccgc
//
LOCUS       DAMZFT010000003        96089 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.003, whole genome shotgun sequence.
ACCESSION   DAMZFT010000003 DAMZFT010000000
VERSION     DAMZFT010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 96089)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 96089)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..96089
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.003"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..768
                     /gene="gpr"
                     /locus_tag="PN977_000214"
     CDS             <1..768
                     /gene="gpr"
                     /locus_tag="PN977_000214"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-glyceraldehyde 3-phosphate reductase"
                     /protein_id="HDI6040299.1"
                     /translation="LIISTKAGYDMWPGPYGSGGSRKYLLASLDQSLKRMGLEYVDIF
                     YSHRVDENTPMEETASALAHAVQSGKALYVGISSYSPERTQKMVELLREWKIPLLIHQ
                     PSYNLLNRWVDKSGLLDTLQNNGVGCIAFTPLAQGLLTGKYLNGIPQDSRMHREGNKV
                     RGLTPKMLTEANLNSLRLLNEMAQQRGQSMAQMALSWLLKDDRVTSVLIGASRAEQLE
                     ENVQALNNLTFSTEELAQIDQHIADGELNLWQASSDK"
     gene            complement(808..1302)
                     /locus_tag="PN977_000215"
     CDS             complement(808..1302)
                     /locus_tag="PN977_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00645 family protein"
                     /protein_id="HDI6040300.1"
                     /translation="MERFLENAMYASRWLLAPVYFGLSLALVALALKFFQEIIHVLPN
                     IFSMAESDLILVLLSLVDMTLVGGLLVMVMFSGYENFVSQLDISENKEKLNWLGKMDA
                     TSLKNKVAASIVAISSIHLLRVFMDAKNVPDNKLMWYVIIHLTFVLSAFVMGYLDRLT
                     RHNH"
     gene            1493..2377
                     /gene="yghA"
                     /locus_tag="PN977_000216"
     CDS             1493..2377
                     /gene="yghA"
                     /locus_tag="PN977_000216"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HDI6040301.1"
                     /translation="MSHLKDPTTQYYTGEYPKQKQPTPGIQAKMTPVPDCGEKIYVGS
                     GRLKDRKALVTGGDSGIGRAAAIAYAREGADVAISYLPVEEEDAQDVKKIIEECGRKA
                     VLLPGDLSDEKFARSLVHEAHKALGGLDIMALVAGKQVAIPDIADLTSEQFQKTFAIN
                     VFALFWLTQEAIPLLPKGASIITTSSIQAYQPSPHLLDYAATKAAILNYSRGLAKQLA
                     EKGIRVNIVAPGPIWTALQISGGQTQDKIPQFGQQTPMKRAGQPAELAPVYVYLASQE
                     SSYVTAEVHGVCGGEHLG"
     gene            complement(2415..2840)
                     /gene="exbD"
                     /locus_tag="PN977_000217"
     CDS             complement(2415..2840)
                     /gene="exbD"
                     /locus_tag="PN977_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein ExbD"
                     /protein_id="HDI6040302.1"
                     /translation="MAMHLNENLDDNGEMHDINVTPFIDVMLVLLIIFMVAAPLATVD
                     VKVNLPASTSTPQPRPEKPVYLSVKADNSMFIGNDPVTDETMITALNALTEGKKDTTI
                     FFRADKTVDYETLMKVMDTLHQAGYLKIGLVGEETAKAK"
     gene            complement(2847..3581)
                     /gene="exbB"
                     /locus_tag="PN977_000218"
     CDS             complement(2847..3581)
                     /gene="exbB"
                     /locus_tag="PN977_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HDI6040303.1"
                     /translation="MGNNLMQTDLSVWGMYQHADIVVKCVMIGLILASVVTWAIFFSK
                     SVEFFNQKRRLKREQQLLAEARSLNQANDIAADFGSKSLSLHLLNEAQNELELSEGSD
                     DNEGIKERTSFRLERRVAAVGRQMGRGNGYLATIGAISPFVGLFGTVWGIMNSFIGIA
                     QTQTTNLAVVAPGIAEALLATAIGLVAAIPAVVIYNVFARQIGGFKAMLGDVAAQVLL
                     LQSRDLDLEASAAAHPVRVAQKLRAG"
     gene            3833..5020
                     /gene="metC"
                     /locus_tag="PN977_000219"
     CDS             3833..5020
                     /gene="metC"
                     /locus_tag="PN977_000219"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HDI6040304.1"
                     /translation="MADKKLDTQLVNAGRSKKYTLGAVNSVIQRASSLVFDSVEAKKH
                     ATRNRANGELFYGRRGTLTHFSLQQAMCELEGGAGCALFPCGAAAVANSILAFVEQGD
                     HVLMTNTAYEPSQDFCSKILSKLGVTTSWFDPLIGADIVKHLQPNTKIVFLESPGSIT
                     MEVHDVPAIVAAVRSVVPDAIIMIDNTWAAGVLFKALDFGIDVSIQAATKYLVGHSDA
                     MIGTAVCNARCWEQLRENAYLMGQMVDADTAYITSRGLRTLGVRLRQHHESSLKVAEW
                     LAEHPQVARVNHPALPGSKGHEFWKRDFTGSSGLFSFVLKKKLNDEELANYLDNFSLF
                     SMAYSWGGYESLILANQPEHIAAIRPQGEIDFSGTLIRLHIGLEDVDDLIADLDAGFA
                     RIV"
     gene            5160..5819
                     /locus_tag="PN977_000220"
     CDS             5160..5819
                     /locus_tag="PN977_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HDI6040305.1"
                     /translation="MAVIQDIIAALWQHDFAALADPHIVSVVYFVMFATLFLENGLLP
                     ASFLPGDSLLILAGALIAQGVMDFLPTIAILTAAASLGCWLSYIQGRWLGNTKTVKGW
                     LAQLPAKYHQRATCMFDRHGLLALLAGRFLAFVRTLLPTMAGISGLPNRRFQFFNWLS
                     GLLWVSVVTSFGYALSMIPFVKRHEDQVMTFLMILPIALLTAGLLGTLFVVIKKKYCN
                     A"
     gene            complement(5859..6758)
                     /gene="yqhC"
                     /locus_tag="PN977_000221"
     CDS             complement(5859..6758)
                     /gene="yqhC"
                     /locus_tag="PN977_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001407451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YqhC"
                     /protein_id="HDI6040306.1"
                     /translation="MKREEICRLLADKVNKLKNKENSLSELLPDVRLLYGETPFARTP
                     VMYEPGIIILFSGHKIGYINERVFRYDANEYLLLTVPLPFECETYATSEVPLAGLRLN
                     VDILQLQELLMDIGEDEHFQPSMAASGINSATLSEEILCAAERLLDVMERPLDARILG
                     KQIIREILYYVLTGPCGGALLALVSRQTHFSLISRVLKRIENKYTENLSVEQLAAEAN
                     MSVSAFHHNFKSVTSTSPLQYLKNYRLHKARMMIIHDGMKASAAAMRVGYESASQFSR
                     EFKRYFGVTPGEDAARMRAMQGN"
     gene            6952..8115
                     /gene="yqhD"
                     /locus_tag="PN977_000222"
     CDS             6952..8115
                     /gene="yqhD"
                     /locus_tag="PN977_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HDI6040307.1"
                     /translation="MNNFNLHTPTRILFGKGAIAGLREQIPHDARVLITYGGGSVKKT
                     GVLDQVLDALKGMDVLEFGGIEPNPAYETLMNAVKLVREQKVTFLLAVGGGSVLDGTK
                     FIAAAANYPENIDPWHILQTGGKEIKSAIPMGCVLTLPATGSESNAGAVISRKTTGDK
                     QAFHSAHVQPVFAVLDPVYTYTLPPRQVANGVVDAFVHTVEQYVTKPVDAKIQDRFAE
                     GILLTLIEDGPKALKEPENYDVRANVMWAATQALNGLIGAGVPQDWATHMLGHELTAM
                     HGLDHAQTLAIVLPALWNEKRDTKRAKLLQYAERVWNITEGSDDERIDAAIAATRNFF
                     EQLGVPTHLSDYGLDGSSIPALLKKLEEHGMTQLGENHDITLDVSRRIYEAAR"
     gene            8220..9047
                     /gene="dkgA"
                     /locus_tag="PN977_000223"
     CDS             8220..9047
                     /gene="dkgA"
                     /locus_tag="PN977_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgA"
                     /protein_id="HDI6040308.1"
                     /translation="MANPTVIKLQDGNVMPQLGLGVWQASNEEVITAIQKALDVGYRS
                     IDTAAAYKNEEGVGKALKNASVNREELFITTKLWNDDHKRPREALLDSLKKLQLDYID
                     LYLMHWPVPAIDHYVEAWKGMIELQKEGLIKSIGVCNFQIHHLQRLIDETGVTPVINQ
                     IELHPLMQQRQLHAWNATHKIQTESWSPLAQGGKGVFDQKVIRDLADKYGKTPAQIVI
                     RWHLDSGLVVIPKSVTPSRIAENFDVWDFRLDKDELGEIAKLDQGKRLGPDPDQFGG"
     gene            9247..10173
                     /locus_tag="PN977_000224"
     CDS             9247..10173
                     /locus_tag="PN977_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjP/YqhG family protein"
                     /protein_id="HDI6040309.1"
                     /translation="MKIILLFLAALASFTVQAQPSSQTVEQTVRQIYQNYKSDASTPY
                     FGETGERAITSARIQQALTLNDNLTLPGNIGWLDYDPVCDCQDFGDLVLESVAITQPD
                     ADHADAVVRFRIFKDDKEKTTQTLKMVAENGRWVIDDIVSNHGSVLQAVNSENEKTLA
                     AIASLQKEQPEAFVAELFEHIADYSWPWTWVVSDSYRQAVNAFYKTTFKTTNNPDEDM
                     QIERQFIYDNPICFGEESLFSRVDEIRVLEKTADSARIHVRFTLSNGNNEEQELVLQR
                     REGKWEIADFIRPNSGSLLKQIEAKTAARLKQ"
     gene            10224..10481
                     /gene="yqhH"
                     /locus_tag="PN977_000225"
     CDS             10224..10481
                     /gene="yqhH"
                     /locus_tag="PN977_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YqhH"
                     /protein_id="HDI6040310.1"
                     /translation="MKTIFTVGAVVLATCLLSGCVNEQKVNQLASNVQTLNAKIARLE
                     QDMKALRPQIYAAKSEANRANTRLDAQDYFDCLRCLRMYAE"
     gene            complement(10523..12742)
                     /locus_tag="PN977_000226"
     CDS             complement(10523..12742)
                     /locus_tag="PN977_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HDI6040311.1"
                     /translation="MSSISLIQPDRDLFSWPQYWAACFGPAPFLPMSREEMDQLGWDS
                     CDIILVTGDAYVDHPSFGMVICGRMLEAQGFRVGIIAQPDWSSKDDFMRLGKPNLFFG
                     VTAGNMDSMINRYTADRRLRHDDAYTPDNVAGKRPDRATLVYTQRCKEAWKDVPVILG
                     GIEASLRRTAHYDYWSDTVRRSVLVDSKADMLMFGNGERPLVEVAHRLAMGEPISEIR
                     DVRNTAIIVKEALPGWSGVDSTRLDTPGKIDPIPHPYGEDLPCADNKPVAPKKQEAKA
                     VTVQPPRPKPWEKTYVLLPSFEKVKGDKVLYAHASRILHHETNPGCARALMQKHGDRY
                     VWINPPAIPLSTEEMDSVFALPYKRVPHPAYGNARIPAYEMIRFSVNIMRGCFGGCSF
                     CSITEHEGRIIQSRSEDSIINEIEAIRDTVPGFTGVISDLGGPTANMYMLRCKSPRAE
                     QTCRRLSCVYPDICPHMDTNHEPTINLYRRARDLKGIKKILIASGVRYDIAVEDPRYI
                     KELATHHVGGYLKIAPEHTEEGPLSKMMKPGMGSYDRFKELFDTYSKQAGKEQYLIPY
                     FISAHPGTRDEDMVNLALWLKKHRFRLDQVQNFYPSPLANSTTMYYTGKNPLAKIGYK
                     SEDVFVPKGDKQRRLHKALLRYHDPANWPLIRQALEAMGKKHLIGSRRDCLVPAPTIE
                     EMREARRQNRNTRPALTKHTPMATQRQTPATAKKASSTQSRLQNAGAKKRPKAAVGR"
     gene            12994..13743
                     /locus_tag="PN977_000227"
     CDS             12994..13743
                     /locus_tag="PN977_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000438650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HDI6040312.1"
                     /translation="MEQVITKRRYYDIGLQIEELLYSGVFKAGERLPSERELSERFNT
                     SRTTIREAIIMLELKGVLNVKQGSGIFFVDSTDKLNQKSLMPYSEIGPFELLQARQVI
                     ESNITGFAASQISFNELQELKKIIRLQENAIAAESDKFEDLDHRFHSIIAEATQNRVL
                     IKQAAELWRAVRTENPRWKKLNYKYLHEKHLRLQWLEDHRAIFLALQQKDSELAREAS
                     WRHLENSKNELIKIFKQDASISDFDDFFFAR"
     gene            14066..15538
                     /locus_tag="PN977_000228"
     CDS             14066..15538
                     /locus_tag="PN977_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HDI6040313.1"
                     /translation="MSGNESIAAAHYDQVTTWPRDGLQADIVHIGFGAFHRGHQAVYT
                     DLTNQLSDTRWGIFEINLFGDAQLIENLNAQNGLFSVVETSASQSTSRLVRSVAGGIH
                     TPRDGIAAAIHKLAEPQVKIVSLTITEKGYCLDPQTRSLDLTNGLIQHDLQNPDAPLS
                     AIGVIVCALQQRKATGLAAFSVLSCDNLPDNGHLTRNAVLGFARQLDQPLAQWIEENV
                     SFPGTMVDRIVPAMTESQFALLETKTGYADPCGIVCESFRQWVIEDNFVRGRPQWDKA
                     GAMFVSNVQPYEEMKLRMLNGSHSFLAYNGSLAGYEFIWQCMEDANFRSITHQLMINE
                     QARTLNPDLNINIQEYADLLIERFSNRNVAHRTGQIAMDGSQKLPQRALTPWLKLHQQ
                     KQNNAVLSLLIAGWLHYVIDAVEKSQSAADPMNDQFQALIKEQQDAWQQALALLHLSA
                     IFGDLSNHQPFINEIKIAFANIKNKGIKATISQLLSDEQK"
     gene            15535..16551
                     /locus_tag="PN977_000229"
     CDS             15535..16551
                     /locus_tag="PN977_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000853917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HDI6040314.1"
                     /translation="MKTLICQQPGVMEYVEKDIPTPADNEVLLKIKAVGICGTDIHAF
                     AGRQPFFSYPRVLGHEICAEAVSRGSQCQTAQSGQRYSVIPCIPCGECAACREEKTNC
                     CERVSLYGVHQDGGFSEYLAVREDNLVPLPDEVSDSAGALVECFAIGAHAVRRAEIKA
                     EQNVLVIGAGPIGLATAAIARAKGAHVVVADIDCQRRQHVVDHLAINVFDPTQEGFIA
                     ALSEVFGGELACVVLDATGNKASMSHDVNLIRHGGKIVFIGLYIGELVIDDPTFHKKE
                     TTLLSSRNATREDFALVIELMRSNKIHENLMKNQAFNFFSVGEDYQRNVVENKNMVKG
                     VITF"
     gene            16562..17572
                     /locus_tag="PN977_000230"
     CDS             16562..17572
                     /locus_tag="PN977_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000214530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ldh family oxidoreductase"
                     /protein_id="HDI6040315.1"
                     /translation="MTTVYVSEENLKSLVHHKLHTAGLDTDTTQQVTDVLVHADITGV
                     HSHGVMRVEHYCTRLAAGGLNKAPQFSIEQISPSVAILDSDDGMGHSALISATEHAIK
                     LAQQEGLGFVSVKNTSHCGALSYFAEMITNKGLVAIVMTQTDTCVAPHGGAERFLGTN
                     PIAFGFPVENSHPMIVDMATSATAFGKILHAKETGKHIGEGLAIDKNGYGTTDPYKIE
                     NLLPFGQHKGSGIALAIDALTGVLMNANFGNHIVRMYGDYDKMRKLASLVIAIDPKKL
                     GNPVFAKTMAKMVTELHAVKPAPGVEKVLAPNDPQIHYKEKCQQEGIPVPAGIFHYLA
                     EN"
     gene            17645..18628
                     /locus_tag="PN977_000231"
     CDS             17645..18628
                     /locus_tag="PN977_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000645949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HDI6040316.1"
                     /translation="MKALRPLTASLMLLTSCLLISNTTLAKTTLKLSHNQDKSHAVHK
                     ALSYLADKTKEYSNGELVIRIYPNATLGNERESLELMNSGALQMVKVNAASLESFAPD
                     YSLFSLPFLFRDRDHYYRVLQSDLGKKILNSSESKGFVGITYYDGGARSFYSNKPITK
                     PEDLAGMKIRVQQSPSAIAMMKALGGVATPMAQGELYTALQQGVVDGGENNTVVYSDM
                     RHAEVAKVYSRDEHTMVPDVLIISTNVLNKLGDKERTALLKAADESMMQMKDVIWPAA
                     EKEAYDKMKGMNATVVDVDKSAFKERVKPLYDEFKAKDAQSAKNLELVESM"
     gene            18670..19152
                     /locus_tag="PN977_000232"
                     /pseudo
     CDS             18670..19152
                     /locus_tag="PN977_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001556790.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
     gene            19163..20467
                     /locus_tag="PN977_000233"
     CDS             19163..20467
                     /locus_tag="PN977_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HDI6040317.1"
                     /translation="MDFEYIYPVLILFGSFAVMLAIGVPITFAIGLSSLLSIITALPP
                     DAAISVISQKMTVGLDGFTLLAIPFFVLAGNIMNTGGIARRLVNLAQALVGRLPGSLA
                     HCNILANTLFGAISGSAVASAAAVGGIMSPLQEKEGYDPAFSAAVNIASAPIGLMIPP
                     SNVLIVYSLASGGTSVAALFLAGYLPGILTAAALMFVAALYARRNHYPVAERINFHQF
                     LQVFRESIPSLMLIFIIIGGIIAGVFTPTEASAIAVIYSLALAMIYREITFKKLNDIL
                     LDSVVTSSIVLLLVGCSMGMSWAMTNADVPELINELITSVSDNKWVILFIINIILLIV
                     GTFMDITPAILIFTPIFLPIDQHLGIDPIHFGIIMVFNLTIGLCTPPVGTILFVGCSI
                     GKVSIDRAIKPLLPMFLALFVVMAIICYFPQLSLMLPGLFST"
     gene            complement(20538..21950)
                     /gene="ftsP"
                     /locus_tag="PN977_000234"
     CDS             complement(20538..21950)
                     /gene="ftsP"
                     /locus_tag="PN977_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsP"
                     /protein_id="HDI6040318.1"
                     /translation="MSLSRRQFIQASGIALCAGAVPLKASAAGQQQPLPVPPLLESRR
                     GQPLFMTVQRAHWSFTPGTRASVWGINGRYLGPTIRVWKGDDVKLIYSNRLTENVSMT
                     VAGLQVPGPLMGGPARMMSPNADWAPVLPIRQNAATLWYHANTPNRTAQQVYNGLAGM
                     WLVEDEVSKSLPIPNHYGVDDFPVIIQDKRLDNFGTPEYNEPGSGGFVGDTLLVNGVQ
                     SPYVEVSRGWVRLRLLNASNSRRYQLQMSDGRPLHVISGDQGFLPAPVSVKQLSLAPG
                     ERREILVDMSNGDEVSITCGEAASIVDRIRGFFEPSSILVSTLVLTLRPTGLLPLVTD
                     SLPMRLLPTEIMAGSPIRSRDISLGDDPGINGQLWDVNRIDVTAQQGTWERWTVRADE
                     PQAFHIEGVMFQIRNVNGAMPFPEDRGWKDTVWVDGQVELLVYFGQPSWAHFPFYFNS
                     QTLEMADRGSIGQLLVNPVP"
     gene            complement(22025..22762)
                     /gene="plsC"
                     /locus_tag="PN977_000235"
                     /gene_synonym="parF"
     CDS             complement(22025..22762)
                     /gene="plsC"
                     /locus_tag="PN977_000235"
                     /gene_synonym="parF"
                     /EC_number="2.3.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acylglycerol-3-phosphate O-acyltransferase"
                     /protein_id="HDI6040319.1"
                     /translation="MLYIFRLIITVIYSILVCVFGSIYCLFSPRNPKHVATFGHMFGR
                     LAPLFGLKVECRKPADAESYGNAIYIANHQNNYDMVTASNIVQPPTVTVGKKSLLWIP
                     FFGQLYWLTGNLLIDRNNRTKAHGTIAEVVNHFKKRRISIWMFPEGTRSRGRGLLPFK
                     TGAFHAAIAAGVPIIPVCVSTTSNKINLNRLHNGLVIVEMLPPIDVSQYGKDQVRELA
                     AHCRSIMEQKIAELDKEVAEREAAGKV"
     gene            complement(22996..25254)
                     /gene="parC"
                     /locus_tag="PN977_000236"
     CDS             complement(22996..25254)
                     /gene="parC"
                     /locus_tag="PN977_000236"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HDI6040320.1"
                     /translation="MSDMAERLALHEFTENAYLNYSMYVIMDRALPFIGDGLKPVQRR
                     IVYAMSELGLNASAKFKKSARTVGDVLGKYHPHGDIACYGAMVLMAQPFSYRYPLVDG
                     QGNWGAPDDPKSFAAMRYTESRLSKYSELLLSELGQGTADWVPNFDGTLQEPKMLPAR
                     LPNILLNGTTGIAVGMATDIPPHNLREVAQAAIALIDQPKTTLDQLLDIVQGPDYPTE
                     AEIITSRAEIRKIYENGRGSVRMRAVWKKEDGAVVISALPHQVSGARVLEQIAAQMRN
                     KKLPMVDDLRDESDHENPTRLVIVPRSNRVDMDQVMNHLFATTDLEKSYRINLNMIGL
                     DGRPAVKNLLEILSEWLVFRRDTVRRRLNYRLEKVLKRLHILEGLLVAFLNIDEVIEI
                     IRNEDEPKPALMSRFGLTETQAEAILELKLRHLAKLEEMKIRGEQSELEKERDQLQGI
                     LASERKMNNLLKKELQADAQAYGDERRSPLQEREEAKAMSEHDMLPSEPVTIVLSQMG
                     WVRSAKGHDIDAPGLNYKAGDSFKAAVKGKSNQPVVFVDSTGRSYAIDPITLPSARGQ
                     GEPLTGKLTLPPGATVDHMLMESDDQKLLMASDAGYGFVCTFNDLVARNRAGKALITL
                     PENAHVMPPVVIEDASDMLLAITQAGRMLMFPVSDLPQLSKGKGNKIINIPSAEAARG
                     EDGLAQLYVLPPQSTLTIHVGKRKIKLRPEELQKVTGERGRRGTLMRGLQRIDRVEID
                     SPRRASSGDSEE"
     gene            complement(25392..26999)
                     /locus_tag="PN977_000237"
                     /pseudo
     CDS             complement(25392..26999)
                     /locus_tag="PN977_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417492.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
     gene            complement(27108..27590)
                     /locus_tag="PN977_000238"
     CDS             complement(27108..27590)
                     /locus_tag="PN977_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HDI6040321.1"
                     /translation="MTNLTLDVNIIDFPSIPVAMLPHRCSPELLNYSVAKFIMWRKET
                     GLSPVNQSQTFGVAWDDPATTAPEAFRFDICGSVSEPIPDNRYGVSNGELTGGRYAVA
                     RHVGELDDISHTIWGIIRHWLPASGEKMRKAPILFHYTNLAEGMTEQRLETDVYVPLA
                     "
     gene            complement(27643..28035)
                     /gene="ygiW"
                     /locus_tag="PN977_000239"
     CDS             complement(27643..28035)
                     /gene="ygiW"
                     /locus_tag="PN977_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB fold stress tolerance protein YgiW"
                     /protein_id="HDI6040322.1"
                     /translation="MKKYAAVIAVMALCSAPVMAAEQGGFSGPSATQSQAGGFQGPNG
                     SVTTIESAKSLRDDTWVTLRGNIVERISDDLYVFKDASGTINVDIDHKRWNGMTVTPK
                     DTVEIQGEVDKDWNSVEIDVKQIRKVNP"
     gene            28187..28846
                     /gene="qseB"
                     /locus_tag="PN977_000240"
     CDS             28187..28846
                     /gene="qseB"
                     /locus_tag="PN977_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator QseB"
                     /protein_id="HDI6040323.1"
                     /translation="MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAP
                     YDAVILDLTLPGMDGRDILREWREKGQREPVLILTARDALAERVEGLRLGADDYLCKP
                     FALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
                     LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGE
                     K"
     gene            28843..30192
                     /gene="qseC"
                     /locus_tag="PN977_000241"
     CDS             28843..30192
                     /gene="qseC"
                     /locus_tag="PN977_000241"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     QseC"
                     /protein_id="HDI6040324.1"
                     /translation="MKFTQRLSLRVRLTLIFLILASVTWLLSSFVAWKQTTDNVDELF
                     DTQLMLFAKRLSTLDLNEINAADRMAQTPNKLKHGHVDDDALTFAIFTHDGRMVLNDG
                     DNGEDIPYSYQREGFADGQLVGDKDQWRFVWMTSPDGKYRIVVGQEWEYREDMALAIV
                     AGQLIPWLVALPIMLIIMMVLLGRELAPLNKLALALRMRDPDSEKPLNATGVPSEVRP
                     LVESLNQLFARTHAMMVRERRFTSDAAHELRSPLTALKVQTEVAQLSDDDPQARKKAL
                     LQLHSGIDRATRLVDQLLTLSRLDSLDNLQDVAEIPLEDLLQSSVMDIYHTAQQAKID
                     VRLTLNAHGIKRTGQPLLLSLLVRNLLDNAVRYSPQGCVVDVTLNADNFIVKDNGPGV
                     TPEALARIGERFYRPPGQTATGSGLGLSIVQRIAKLHDMNVEFGNAEQGGFEAKVSW"
     gene            30302..30883
                     /gene="mdaB"
                     /locus_tag="PN977_000242"
     CDS             30302..30883
                     /gene="mdaB"
                     /locus_tag="PN977_000242"
                     /EC_number="1.6.5.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone oxidoreductase MdaB"
                     /protein_id="HDI6040325.1"
                     /translation="MSNILIINGAKKFAHSNGQLNDTLTEVADGTLRDLGHDVRIVRA
                     DSDYDVKAEVQNFLWADVVIWQMPGWWMGAPWTVKKYIDDVFTEGHGTLYASDGRTRK
                     DPSKKYGSGGLVQGKKYMLSLTWNAPMEAFTEKDQFFHGVGVDGVYLPFHKANQFLGM
                     EPLPTFIANDVIKMPDVPRYTEEYRKHLVEIFG"
     gene            30914..31228
                     /locus_tag="PN977_000243"
     CDS             30914..31228
                     /locus_tag="PN977_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HDI6040326.1"
                     /translation="MITVIAEIRTRPGQHHRQAVLDQFAKIVPTVLKEEGCHGYAPMV
                     DCAAGVSFQSMAPDSIVMIEQWESIAHLEAHLQTPHMKAYSEAVKGDVLEMNIRILQP
                     GI"
     gene            complement(31273..32160)
                     /locus_tag="PN977_000244"
     CDS             complement(31273..32160)
                     /locus_tag="PN977_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HDI6040327.1"
                     /translation="MIQKDKARVDIFGERFRTRASQLTPGLRVVASYINEHREVVLEQ
                     TAMEIAATLNTSDATVIRAIQALGFAGLRDLKRTLEQWLGPALSSSEKMSTTVSNLTS
                     DVNTAIDFVLEGHLYTCNVLSEPENRHALAQAVALLVQARQVAIFGIGASGILADYTA
                     RLFNRIGLPATALNRTGIGLAEQLIALQRGDVLIMMAQKSAHREGLTTLREARRLGIP
                     VILLTNALDSRFSKDASIVIHVPRGDEKGKTPLHGTVLLCLEMIVWSVASAVPQRAVK
                     TIKRINDFHRGLKTGRKNG"
     gene            complement(32157..33104)
                     /locus_tag="PN977_000245"
     CDS             complement(32157..33104)
                     /locus_tag="PN977_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-siderophore ABC transporter
                     substrate-binding protein"
                     /protein_id="HDI6040328.1"
                     /translation="MRLFFSLLILLSFFSRATEPVQVFTDDLGRKVTVPAHPKRIVSL
                     HDLDITIPLIELGVPPVASHGRTRPDGSHFIRSGALLTGVDFDNSSIAFIGTADIDIE
                     AIVAAKPDLIITEPTRNTPIEQLEKIAPTVSIDHLKGGAPEIYRKLAELTGTQSQLAI
                     LERRYQAQINALKATLDSQKITVSVIQANQGKINVMHSYHSLGRVLRDAGFRFPPLIE
                     SIPEGGRMDVSAERLPELDADFVFATWRGDTGGKPQDELAAMEKVMPGWCQFLTACRS
                     GRYVLISREEAISNSFASLGLMAAQIQSQIAGRPLPEDK"
     gene            complement(33115..34152)
                     /locus_tag="PN977_000246"
     CDS             complement(33115..34152)
                     /locus_tag="PN977_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311941.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HDI6040329.1"
                     /translation="MNVGLRPLRVGKFSTLVRPKNLLLLGGLFLLAVGILIFGLMQGS
                     FSVPASEVGRALFAPENVSTDARYIVQDIRLPRVIMALLCGAMLGMAGAAMQSIARNG
                     LADPGLIGVKEGCSVAVLWLIFQFPMLGMFWRPVAGLAGGLLVALIVIFCARDISRPR
                     FVLIGIGVSWFFAAGIGVFMTTADVRDVQTALMWLSGSLHAANWMLVAISACWMLPAA
                     LLLLFTARTADIALLGHQVATGLGVNSSRLALLRVAAPIILTAVCVSCVGNIGFVGLI
                     APHISRFILRGGQTTLLLGSAVSGALLVILADSIGRLAFLPLQLPAGIIISLIGGPFF
                     LLLLWQRRNSF"
     gene            complement(34161..35144)
                     /locus_tag="PN977_000247"
     CDS             complement(34161..35144)
                     /locus_tag="PN977_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HDI6040330.1"
                     /translation="MMRTGLVTVIFLALLLVGCVVYPGIGARFIAPQTVLQAFLHFDP
                     QNFDHNVIVRLRLPRLAAALLTGASLGVAGALLQAVIRNPLGEPHILGLNAGAALAVV
                     AASALGLAFPVGRPLLASTGGALLFLLILLLSSAGRSGLTPMKVTLCGVALSAFVSSI
                     TAAILILDEQTLLAMRTWLAGDLAGQDWATLGTSAWFSLGGFVLALYLAPSLNMLALG
                     DRMAQGLGVSVFRTRTFTLLAIALLCGAAVSIAGPIGFVGLLVPQIVRRLVSADLRVL
                     LPLSACVGALLLLLADIIARTLFTPHELATGVMTALVGAPVFVIMATRMFK"
     gene            complement(35141..35950)
                     /locus_tag="PN977_000248"
     CDS             complement(35141..35950)
                     /locus_tag="PN977_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDI6040331.1"
                     /translation="MAHNAKGQGLILDNVSAGYHKKIIVDGVSFSVPTEKMTVLVGAN
                     GCGKSTLLSTIARILQPMGGSILLDGKAIHEQPTKALSRRLGILPQSPLLPEGLTVYE
                     LVSRGRFPWQNFIRQWSDADELAVEEALKLTGTQEFAHLPVEKLSGGQRQRCWIAMVL
                     AQKTPYILLDEPTTWLDLRYQVEILELLHDLTRHHGRTVVVVLHDLNFAVNYGDTLIF
                     LRQGKVVRVLNEGEHCTPELVKAVFDVDVHASINPLTGKPFFMPFRGVEKV"
     gene            36324..38465
                     /locus_tag="PN977_000249"
     CDS             36324..38465
                     /locus_tag="PN977_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent siderophore receptor"
                     /protein_id="HDI6040332.1"
                     /translation="MAMFTPSFSGLKGRALFSLLFAAPMIHATDSVTTKDGETITVTA
                     DANTATEATDGYQPLSTSTATLTDMPMLDIPQVVNTVSDQVLENQNATTLDEALYNVS
                     NVVQTNTLGGTQDAFVRRGFGANRDGSIMTNGLRTVLPRSFNAATERVEVLKGPASTL
                     YGILDPGGLINVVTKRPEKTFHGSVSATSSSFGGGTGQLDITGPIEGTQLAYRLTGEV
                     QDEDYWRNFGKERSTFIAPSLTWFGDNATVTMLYSHRDYKTPFDRGTIFDLTTKQPVN
                     VDRKIRFDEPFNITDGKSDLAQLNAEYHLNSQWTARFDYSYSQDKYSDNQARVTAYDA
                     TTGTLTRRVDATQGSTQRMHATRADLQGNVDIAGFYNEILGGVSYEYYDLLRTDMIRC
                     KKAKDFNIYNPVYGNTSKCTTVSASDSDQTIKQESYSAYAQDALYLTDNWIAVAGIRY
                     QYYTQYAGKGRPFNVNTDSRDEQWTPKLGLVYKLTPSVSLFANYSQTFMPQSSIASYI
                     GDLPPESSNAYEVGAKFELFDGITADIALFDIHKRNVLYTESVGDETIAKTAGRVRSR
                     GVEVDLAGALTENINIIASYGYTDAKVLEDPDYAGKPLPNVPRHTGSLFLTYDIHNMP
                     GNNTLTFGGGGHGVSRRSATNGADYYLPGYFVADAFAAYKMKLQYPVTLQLNVKNLFD
                     KTYYTSSIATNNLGNQIGDPREVQFTVKMEF"
     gene            complement(38529..40421)
                     /gene="parE"
                     /locus_tag="PN977_000250"
     CDS             complement(38529..40421)
                     /gene="parE"
                     /locus_tag="PN977_000250"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HDI6040333.1"
                     /translation="MTQTYNADAIEVLTGLEPVRRRPGMYTDTTRPNHLGQEVIDNSV
                     DEALAGHAKRVDVILHADQSLEVIDDGRGMPVDIHPEEGVPAVELILCRLHAGGKFSN
                     KNYQFSGGLHGVGISVVNALSKRVEVNVRRDGQIYNIAFENGEKVQDLQVIGTCGKRN
                     TGTSVHFWPDETFFDSPRFSVSRLTHVLKAKAVLCPGVEITFKDEINNTEQRWCYQDG
                     LNDYLAEAVNGLPTLPEKPFIGNFAGDTEAVDWALLWLPEGGELLTESYVNLIPTMQG
                     GTHVNGLRQGLLDAMREFCEYRNILPRGVKLSAEDIWDRCAYVLSVKMQDPQFAGQTK
                     ERLSSRQCAAFVSGVVKDAFTLWLNQNVQAAELLAEMAISSAQRRMRAAKKVVRKKLT
                     SGPALPGKLADCTAQDLNRTELFLVEGDSAGGSAKQARDREYQAIMPLKGKILNTWEV
                     SSDEVLASQEVHDISVAIGIDPDSDDLSQLRYGKICILADADSDGLHIATLLCALFVK
                     HFRALVKHGHVYVALPPLYRIDLGKEVYYALTEEEKEGVLEQLKRKKGKPNVQRFKGL
                     GEMNPMQLRETTLDPNTRRLVQLTIDDEDDQRTDAMMDMLLAKKRSEDRRNWLQEKGD
                     MAEIEV"
     gene            complement(40450..41031)
                     /gene="yqiA"
                     /locus_tag="PN977_000251"
     CDS             complement(40450..41031)
                     /gene="yqiA"
                     /locus_tag="PN977_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase YqiA"
                     /protein_id="HDI6040334.1"
                     /translation="MSTLLYLHGFNSSPRSAKASLLKNWLAEHHPDVEMIIPQLPPYP
                     SDAAELLESIVLEHGGDSLGIVGSSLGGYYATWLSQCFMLPAVVVNPAVRPFELLTDY
                     LGQNENPYTGQQYVLESRHIYDLKVMQIDPLEAPDLIWLLQQTGDEVLDYRQAVAYYA
                     SCRQTVIEGGNHAFTGFEDYFNPIVDFLGLHHL"
     gene            complement(41031..41858)
                     /gene="cpdA"
                     /locus_tag="PN977_000252"
     CDS             complement(41031..41858)
                     /gene="cpdA"
                     /locus_tag="PN977_000252"
                     /EC_number="3.1.4.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3',5'-cyclic-AMP phosphodiesterase"
                     /protein_id="HDI6040335.1"
                     /translation="MESLLTLPLAGEARVRILQITDTHLFAQKHEALLGVNTWESYQA
                     VLEAIRPHQHEFDLIVATGDLAQDQSSAAYQHFAEGIASFRAPCVWLPGNHDFQPAMY
                     SALQDAGISPAKRVFIGEQWQILLLDSQVFGVPHGELSEFQLEWLERKLADAPERHTL
                     LLLHHHPLPAGCSWLDQHSLRNAGELDTVLAKFPHVKYLLCGHIHQELDLDWNGRRLL
                     ATPSTCVQFKPHCSNFTLDTIAPGWRTLELHADGTLTTEVHRLADARFQPDTASEGY"
     gene            complement(41883..42305)
                     /locus_tag="PN977_000253"
     CDS             complement(41883..42305)
                     /locus_tag="PN977_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1249 family protein"
                     /protein_id="HDI6040336.1"
                     /translation="MKRYTPDFPEMMRLCEMNFSQLRRLLPRNDAPGETVSYQVANAQ
                     YRLTIVESTRYTTLVTIEQTAPAISYWSLPSMTVRLYHDAMVAEVCSSQQIFRFKARY
                     DYPNKKLHQRDEKHQINQFLADWLRYCLAHGAMAIPVY"
     gene            complement(42306..42935)
                     /gene="nudF"
                     /locus_tag="PN977_000254"
     CDS             complement(42306..42935)
                     /gene="nudF"
                     /locus_tag="PN977_000254"
                     /EC_number="3.6.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribose diphosphatase"
                     /protein_id="HDI6040337.1"
                     /translation="MLKPDNLPVTFGKNDVEIIARETLYRGFFSLDLYRFRHRLFNGQ
                     MSHEVRREIFERGHAAVLLPFDPVRDEVVLIEQIRIAAYDTSETPWLLEMVAGMIEEG
                     ESVEDVARREAIEEAGLIVKRTKPVLSFLASPGGTSERSSIMVGEVDATTASGIHGLA
                     DENEDIRVHVVSREQAYQWVEEGKIDNAASVIALQWLQLHHQALKNEWA"
     gene            43140..44621
                     /gene="tolC"
                     /locus_tag="PN977_000255"
     CDS             43140..44621
                     /gene="tolC"
                     /locus_tag="PN977_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane channel protein TolC"
                     /protein_id="HDI6040338.1"
                     /translation="MKKLLPILIGLSLSGFSSLSQAENLMQVYQQARLSNPELRKSAA
                     DRDAAFEKINEARSPLLPQLGLGADYTYSNGYRDANGINSNATSASLQLTQSIFDMSK
                     WRALTLQEKAAGIQDVTYQTDQQTLILNTATAYFNVLNAIDVLSYTQAQKEAIYRQLD
                     QTTQRFNVGLVAITDVQNARAQYDTVLANEVTARNNLDNAVEQLRQITGNYYPELAAL
                     NVENFKTDKPQPVNTLLKEAEKRNLSLLQARLSQDLAREQIRQAQDGHLPTLDLTAST
                     GISDTSYSGSKTRGAAGTQYDDSNMGQNKVGLSFSLPIYQGGMVNSQVKQAQYNFVGA
                     SEQLESAHRSVVQTVRSSFNNINASISSINAYKQAVVSAQSSLDAMEAGYSVGTRTIV
                     DVLDATTTLYNAKQELANARYNYLINQLNIKSALGTLNEQDLLALNNALSKPVSTNPE
                     NVAPQTPEQNAIADGYAPDSPAPVVQQTSARTTTSNGHNPFRN"
     gene            44769..45440
                     /locus_tag="PN977_000256"
     CDS             44769..45440
                     /locus_tag="PN977_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417509.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 family protein"
                     /protein_id="HDI6040339.1"
                     /translation="MKRTKSIRHASFRKNWSARHLTPVALAVATVFMLAGCEKSDETV
                     SLYQNADDCSAANPGKSAECTTAYNNALKEAERTAPKYATREDCVAEFGEGQCQQAPA
                     QAGMAPENQAQAQQSSGSFWMPLMAGYMMGRLMGGGAGFAQQPLFSSKNPASPAYGKY
                     TDATGKNYGAAQPGRTMTVPKTAMAPKPATTTTVTRGGFGESVAKQSTMQRSATGTSS
                     RSMGG"
     gene            45446..46606
                     /locus_tag="PN977_000257"
     CDS             45446..46606
                     /locus_tag="PN977_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HDI6040340.1"
                     /translation="MERVSITERPDWREKAHEYGFNFHTMYGEPYWCEDAYYKLTLAQ
                     VEKLEEVTAELHQMCLKVVEKVIASDELMTKFRIPKHTWSFVRQSWLTHQPSLYSRLD
                     LAWDGTGEPKLLENNADTPTSLYEAAFFQWIWLEDQLNAGNLPEGSDQFNSLQEKLID
                     RFVELREQYGFQLLHLTCCRDTVEDRGTIQYLQDCATEAEIATEFLYIDDIGLGEKGQ
                     FTDLQDQVISNLFKLYPWEFMLREMFSTKLEDAGVRWLEPAWKSIISNKALLPLLWEM
                     FPNHPNLLPAYFAEDDHPQMEKYVVKPIFSREGANVSIIENGKTIEAAEGPYGEEGMI
                     VQQFHPLPKFGDSYMLIGSWLVNDQPAGIGIREDRALITQDMSRFYPHIFVE"
     gene            47091..48887
                     /locus_tag="PN977_000258"
     CDS             47091..48887
                     /locus_tag="PN977_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016159766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aryl-sulfate sulfotransferase"
                     /protein_id="HDI6040341.1"
                     /translation="MFDKYRKTLVAGTVAITLGLSASGVMAAGFKPAPPAGQLGAVIV
                     DPYGNAPLTALVDLDSHVISDVRVTVHGKGEKGVEISYPVGQESLKTYDGVPIFGLYQ
                     KFANKVTVEWKENGKVMKDDYVVHTSAIVNNYMDNRSISDLQQTKVIKVAPGFEDRLY
                     LVNTHTFTAQGSDLHWHGEKDKNAGILDAGPATGALPFDIAPFTFIVDTEGEYRWWLD
                     QNTFYDGRDRNINKRGYLMGIRETPRGTFTAVQGQHWYEFDMMGQVLEDHKLPRGFAD
                     ATHESIETPNGTVLLRVGKSNYRRDDGVHVTTIRDHILEVDKSGRVVDVWDLTKILDP
                     KRDALLGALDAGAVCVNVDLAHAGQQAKLEPDTPFGDALGVGPGRNWAHVNSIAYDAK
                     DDSIILSSRHQGVVKIGRDKQVKWILAPSKGWEKPLASKLLKPVDANGKPITCNENGL
                     CENSDFDFTYTQHTAWISSKGTLTIFDNGDGRHLEQPALPTMKYSRFVEYKIDEKKGT
                     VQQVWEYGKERGYDFYSPITSIIEYQADRNTMFGFGGSIHLFDVGQPTVGKLNEIDYK
                     TKEVKVEIDVLSDKPNQTHYRALLVRPQQMFK"
     gene            48905..49573
                     /locus_tag="PN977_000259"
     CDS             48905..49573
                     /locus_tag="PN977_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA/DsbL"
                     /protein_id="HDI6040342.1"
                     /translation="MSKLGISSLFKSILLTAALAVSFTASAFTEGTDYMVLEKPIPNA
                     DKTLIKVFSYACPFCYKYDKAVTGPVSEKVKDIVAFTPFHLETKGEYGKQASEVFAVL
                     INKDKAAGISLFDANSQFKKAKFAYYVAYHDKKERWSDGKDPAAFIKTGLDAAGMSQA
                     DFEAALKEPAVQETLEKWKASYDVAKIQGVPAYVVNGKYLIYTKSIKSIDAMADLIRE
                     LASK"
     gene            49588..50259
                     /gene="dsbI"
                     /locus_tag="PN977_000260"
     CDS             49588..50259
                     /gene="dsbI"
                     /locus_tag="PN977_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000511507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide oxidoreductase DsbI"
                     /protein_id="HDI6040343.1"
                     /translation="MGIKGMWKDLRTSPVDTLVRWQEQRLLWLLMAVAMGALIILAHS
                     FFQIYLYMAPCEQCVYIRYAMFVMVIGGLVAAINPKNIILKLIGCVMAFYGSILGLKF
                     SLKLNDIHHAVHNPDPDSLFGVQGCSTDPTFPFNLPLAQWAPNWFKPTGDCGYDAPIV
                     PDGVTLSSTQQWFVEMYQQSEGWYLLPPWHFMNMAQACMLAFGMCLVLLVIMSGAWAL
                     KIIRG"
     gene            complement(50305..51093)
                     /gene="ygiD"
                     /locus_tag="PN977_000261"
     CDS             complement(50305..51093)
                     /gene="ygiD"
                     /locus_tag="PN977_000261"
                     /EC_number="1.13.11.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001356237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4,5-DOPA dioxygenase extradiol"
                     /protein_id="HDI6040344.1"
                     /translation="MSSTRMPALFLGHGSPMNVLEDNLYTRSWQTLGMTLPRPQAIVV
                     VSAHWFTRGTGVTAMETPPTIHDFGGFPQALYDTHYPAPGSPALAQHLVELLAPVPVT
                     LDKEAWGFDHGSWGVLIKMYPDADIPMVQLSIDSSKPAAWHFEMGRKLAALRDEGIML
                     VASGNVVHNLRTVKWHGDSSPYPWATSFNEYVKANLTWQGPVEQHPLVNYLDHEGGAL
                     SNPTPEHYLPLLYVLGAWDGQEPITIPVDDIEMGSLSMLSVQIG"
     gene            51236..52009
                     /gene="zupT"
                     /locus_tag="PN977_000262"
     CDS             51236..52009
                     /gene="zupT"
                     /locus_tag="PN977_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HDI6040345.1"
                     /translation="MSVPLILTILAGAATFIGAFLGVLGQKPSNRLLAFSLGFAAGIM
                     LLISLMEMLPAALAAEGMSPVLGYGMFIFGLLGYFGLDRMLPHAHPQDLMQKSVQPLP
                     KSIKRTAILLTLGISLHNFPEGIATFVTASSNLELGFGIALAVALHNIPEGLAVAGPV
                     YAATGSKRTAILWAGISGLAEILGGVLAWLILGSMISPVVMAAIMAAVAGIMVALSVD
                     ELMPLAKEIDPNNNPSYGVLCGMSVMGFSLVLLQTAGIG"
     gene            complement(52067..52237)
                     /locus_tag="PN977_000263"
     CDS             complement(52067..52237)
                     /locus_tag="PN977_000263"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6040346.1"
                     /translation="MFIAWYWIVLIALVVVGYFLHLKRYCRAFRQDRDALLEARNKYL
                     NSTREETAEKVE"
     gene            complement(52499..53152)
                     /gene="ribB"
                     /locus_tag="PN977_000264"
     CDS             complement(52499..53152)
                     /gene="ribB"
                     /locus_tag="PN977_000264"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HDI6040347.1"
                     /translation="MNQTLLSSFGTPFERVENALAALREGRGVMVLDDEDRENEGDMI
                     FPAETMTVEQMALTIRHGSGIVCLCITEDRRKQLDLPMMVENNTSAYGTGFTVTIEAA
                     EGVTTGVSAADRITTVRAAIADDAKPSDLNRPGHVFPLRAQAGGVLTRGGHTEATIDL
                     MTLAGFKPAGVLCELTNDDGTMARAPECIEFANKHNMALVTIEDLVAYRQAHERKAS"
     regulatory      complement(53256..53404)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            53526..53825
                     /gene="ubiK"
                     /locus_tag="PN977_000265"
     CDS             53526..53825
                     /gene="ubiK"
                     /locus_tag="PN977_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311957.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis accessory factor UbiK"
                     /protein_id="HDI6040348.1"
                     /translation="MIDPKKIEQIARQVHESMPKGIREFGEDVEKKIRQTLQAQLTRL
                     DLVSREEFDVQTQVLLRTREKLALLEQRISELEARNNSVADLQSPPAIPPIDKAE"
     gene            complement(53860..54060)
                     /gene="glgS"
                     /locus_tag="PN977_000266"
     CDS             complement(53860..54060)
                     /gene="glgS"
                     /locus_tag="PN977_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface composition regulator GlgS"
                     /protein_id="HDI6040349.1"
                     /translation="MDHSLNSLNNFDFLARSFARMHAEGRPVDILAVTGNMDEEHRTW
                     FCARYAWYCQQMMQTRELELEH"
     gene            54329..54958
                     /locus_tag="PN977_000267"
     CDS             54329..54958
                     /locus_tag="PN977_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1449 family protein"
                     /protein_id="HDI6040350.1"
                     /translation="MILFADYNTPYLFAISFVLLIGLLEIFALICGHMLSGALDAHLD
                     HYDSITTGHISQALHYLNIGRLPALVVLCLLAGFFGLIGILLQHTCIMVWQSPLSNLF
                     VVPVSLLFTIIAVHYTGKIVAPWIPRDHSSAITEEEYIGSMALITGHQATSGNPCEGK
                     LTDQFGQIHYLLLEPEEGKIFTKGDKVLIICRLSATRYLAENNPWPQIL"
     gene            54985..56646
                     /locus_tag="PN977_000268"
     CDS             54985..56646
                     /locus_tag="PN977_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HDI6040351.1"
                     /translation="MDDIVNSVPSWMFTAIIAVCILFIIGIIFARLYRRASAEQAFVR
                     TGLGGQKVVMSGGAIVMPIFHEIIPINMNTLKLEVSRSTIDSLITKDRMRVDVVVAFF
                     VRVKPSVEGIATAAQTLGQRTLSPEDLRMLVEDKFVDALRATAAQMTMHELQDTRENF
                     VQGVQNTVAEDLSKNGLELESVSLTNFNQTSKEHFNPNNAFDAEGLTKLTQETERRRR
                     ERNEVEQDVEVAVREKNRDALSRKLEIEQQEAFMTLEQEQQVKTRTAEQNAKIAAFEA
                     ERRREAEQTRILAERQIQETEIDREQAVRSRKVEAEREVRIKEIEQQQVTEIANQTKS
                     IAIAAKSEQQSQAEARANLALAEAVSAQQNVETTRQTAEADRAKQVALIAAAQDAETK
                     AVELTVRAKAEKEAAEMQAAAIVELAEATRKKGLAEAEAQRALNDAINVLSDEQTSLK
                     FKLALLQSLPAVIEKSVEPMKSIDGIKIIQVDGLNRGGAAGDAASGSVSGGNLAEQAL
                     SAALSYRTQAPLIDSLLNEIGVSGGSLAALTSPLTSTTPVAENVE"
     gene            56885..56944
                     /locus_tag="PN977_000269"
     CDS             56885..56944
                     /locus_tag="PN977_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HDI6040352.1"
                     /translation="MMKLVIILIVLLLVSFATY"
     gene            57261..57320
                     /gene="ibsE"
                     /locus_tag="PN977_000270"
     CDS             57261..57320
                     /gene="ibsE"
                     /locus_tag="PN977_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsE"
                     /protein_id="HDI6040353.1"
                     /translation="MMKFVIILVVLLLLSFPTY"
     gene            complement(57440..58873)
                     /gene="hldE"
                     /locus_tag="PN977_000271"
     CDS             complement(57440..58873)
                     /gene="hldE"
                     /locus_tag="PN977_000271"
                     /EC_number="2.7.1.167"
                     /EC_number="2.7.7.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     D-glycero-beta-D-manno-heptose-7-phosphate
                     kinase/D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase HldE"
                     /protein_id="HDI6040354.1"
                     /translation="MKVTLPEFERAGVMVVGDVMLDRYWYGPTSRISPEAPVPVVKVN
                     TIEERPGGAANVAMNIASLGANARLVGLTGIDDAARALSKSLADVNVKCDFVSVPTHP
                     TITKLRVLSRNQQLIRLDFEEGFEGVDPQPLHERINQALSSIGALVLSDYAKGALASV
                     QQMIQLARKAGVPVLIDPKGTDFERYRGATLLTPNLSEFEAVVGKCKTEEEIVERGMK
                     LIADYELSALLVTRSEQGMSLLQPGKAPLHMPTQAQEVYDVTGAGDTVIGVLAATLAA
                     GNSLEEACFFANAAAGVVVGKLGTSTVSPIELENAVRGRADTGFGVMTEEELKLAVAA
                     ARKRGEKVVMTNGVFDILHAGHVSYLANARKLGDRLIVAVNSDASTKRLKGDSRPVNP
                     LEQRMIVLGALEAVDWVVSFEEDTPQRLIAGILPDLLVKGGDYKPEEIAGSKEVWANG
                     GEVLVLNFEDGCSTTNIIKKIQQDKKG"
     gene            complement(58921..61761)
                     /gene="glnE"
                     /locus_tag="PN977_000272"
     CDS             complement(58921..61761)
                     /gene="glnE"
                     /locus_tag="PN977_000272"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HDI6040355.1"
                     /translation="MKPLSSPLQQYWQTVVERLPEPLADESLSAQAKSVLTFSDFVQD
                     SVIAHPEWLTELESQPPQADEWQHYSAWWQEALSNVSDEAGLMRELRLFRRRIMVRIA
                     WAQTLALVTEESILLQLSHLAETLIVAARDWLYDACCREWGTPCNAQGEAQPLLILGM
                     GKLGGGELNFSSDIDLIFAWPEHGCTQGGRRELDNAQFFTRMGQRLIKVLDQPTQDGF
                     VYRVDMRLRPFGESGPLVLSFAALEDYYQEQGRDWERYAMVKARIMGDSEGVYANELR
                     AMLRPFVFRRYIDFSVIQSLRNMKGMIAREVRRRGLTDNIKLGAGGIREIEFIVQVFQ
                     LIRGGREPSLQSRSLLPTLSAIAELHLLSENDAEQLRVAYLFLRRLENLLQSINDEQT
                     QTLPSDELNRARLAWAMDFADWQQLTGALIGHMTNVRRVFNELIGDDESETQEESLSE
                     QWRELWQDALQEDDTTPVLAHLSEDDRKQVLTLIADFRKELDKRTIGPRGRQVLDHLM
                     PHLLRDVCTREDAAVTLSRITALLVGIVTRTTYLELLSEFPAALKHLISLCAASPMIA
                     SQLARYPLLLDELLDPNTLYQPTATDAYRDELRQYLLRVPEDDEEQQLEALRQFKQAQ
                     LLRIAAADIAGTLPVMKVSDHLTWLAEAMIDAVVQQAWVQMVARYGKPNHLNEREGRG
                     FAVVGYGKLGGWELGYSSDLDLIFLHDCPMDAMTDGEREIDGRQFYLRLAQRIMHLFS
                     TRTSSGILYEVDARLRPSGAAGMLVTSAEAFADYQKNEAWTWEHQALVRARVVYGDPQ
                     LTAHFDAVRREIMTLPREGKTLQTEVREMREKMRAHLGNKHRDRFDIKADEGGITDIE
                     FITQYLVLRYAHEKPKLTRWSDNVRILELLAQNDIMEEQEAMALTRAYTTLRDELHHL
                     ALQELPGHVPEDCFTAEREQVRVSWQKWLVEE"
     gene            complement(61784..63085)
                     /gene="ygiF"
                     /locus_tag="PN977_000273"
     CDS             complement(61784..63085)
                     /gene="ygiF"
                     /locus_tag="PN977_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic triphosphatase"
                     /protein_id="HDI6040356.1"
                     /translation="MAQEIELKFIVNHSAVEALRDHLNTLGGEHHDPVQLLNIYYETP
                     DNWLRGHDMGLRIRGENGRYEMTMKVAGRVTGGLHQRPEYNVALSEPMLDLAQLPTEV
                     WPNGELPTDLASRVQPLFSTDFYREKWLVEVDGSQIEIALDQGEVKAGEFAEPICELE
                     LELLSGDTRAVLKLANQLVSQTGLRQGSLSKAARGYHLAQGNPAREIKPTTILHVAAK
                     ADVEQGLEAALELALAQWQYHEELWVRGNDAAKEQVLAAIGLVRHTLMLFGGIVPRKA
                     STHLRDLLTQCEATIASVVSAVTAVYSTETAMAKLALTEWLVSKAWQPFLDAKAQSKM
                     SDSFKRFADIHLSRHAAELKSVFCQPLGDRYRDQLPRLTRDIDSILLLAGYYDPVVAQ
                     AWLENWQGLRHAIVTGQRIEIEHFRNEANNQEPFWLHSGKR"
     gene            63327..63947
                     /locus_tag="PN977_000274"
     CDS             63327..63947
                     /locus_tag="PN977_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HDI6040357.1"
                     /translation="MPKLRLIGLTLLALSATAVSHAEETRYVSDELNTWVRSGPGDHY
                     RLVGTVNAGEEVTLLQTDANTNYAQVKDSSGRTAWIPLKQLSTEPSLRSRVPDLENQV
                     KTLTDKLTNIDNTWNQRTAEMQQKVAQSDSVINGLKEENQKLKNELIVAQKKVDAASV
                     QLDDKQRTIIMQWFMYGGGVLGLGLLLGLVLPHLIPSRKRKDRWMN"
     gene            64011..65249
                     /gene="cca"
                     /locus_tag="PN977_000275"
     CDS             64011..65249
                     /gene="cca"
                     /locus_tag="PN977_000275"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused tRNA nucleotidyltransferase/2',3'-cyclic
                     phosphodiesterase/2' nucleotidase/phosphatase Cca"
                     /protein_id="HDI6040358.1"
                     /translation="MKIYLVGGAVRDALLGLPVKDRDWVVVGSTPQEMLDAGYQQVGR
                     DFPVFLHPQTHEEYALARTERKSGSGYTGFTCYAAPDVTLEDDLKRRDLTINALAQDD
                     NGEIIDPYNGLGDLQNRLLRHVSPAFGEDPLRVLRVARFAARYAHLGFRIADETLALM
                     REMTHAGELEHLTPERVWKETESALTTRNPQVFFQVLRDCGALRVLFPEIDALFGVPA
                     PARWHPEIDTGIHTLMTLSMAAMLSPQVDVRFATLCHDLGKGLTPPELWPRHHGHGPA
                     GVKLVEQLCQRLRVPNEIRDLARLVAEFHDLIHTFPMLNPKTIVKLFDSIDAWRKPQR
                     VEQLALTSEADVRGRTGFESADYPQGRWLREAWEVAQSVPTKAVVEAGFKGVEIREEL
                     TRRRIAAVASWKEQRCPKPD"
     gene            complement(65330..66151)
                     /gene="bacA"
                     /locus_tag="PN977_000276"
     CDS             complement(65330..66151)
                     /gene="bacA"
                     /locus_tag="PN977_000276"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HDI6040359.1"
                     /translation="MSDMHSLLIAAILGVVEGLTEFLPVSSTGHMIIVGHLLGFEGDT
                     AKTFEVVIQLGSILAVVVMFWRRLFGLIGIHFGRPLQHEGESKGRLTLIHILLGMIPA
                     VVLGLLFHDTIKSLFNPINVMYALVVGGLLLIAAECLKPKEPRAPGLDDMTYRQAFMI
                     GCFQCLALWPGFSRSGATISGGMLMGVSRYAASEFSFLLAVPMMMGATALDLYKSWGF
                     LTTGDIPMFAVGFITAFVVALIAIKTFLQLIKRISFIPFAIYRFIVAAAVYVVFF"
     gene            complement(66242..66610)
                     /gene="folB"
                     /locus_tag="PN977_000277"
     CDS             complement(66242..66610)
                     /gene="folB"
                     /locus_tag="PN977_000277"
                     /EC_number="4.1.2.25"
                     /EC_number="5.1.99.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional dihydroneopterin
                     aldolase/7,8-dihydroneopterin epimerase"
                     /protein_id="HDI6040360.1"
                     /translation="MDIVFIEQLSVITTIGVYDWEQTIEQKLVFDIEMAWDNRKAAKS
                     DDVADCLSYADIAETVVSHVEGARFALVERVAEEVAELLLARFNSPWVRIKLSKLGAV
                     ARAANVGVIIERGNNLKENN"
     gene            66715..67332
                     /gene="plsY"
                     /locus_tag="PN977_000278"
     CDS             66715..67332
                     /gene="plsY"
                     /locus_tag="PN977_000278"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HDI6040361.1"
                     /translation="MSAIAPGMILIAYLCGSISSAILVCRLCGLPDPRTSGSGNPGAT
                     NVLRIGGKGAAVAVLIFDVLKGMLPVWGAYELGVSPFWLGLIAIAACLGHIWPVFFGF
                     KGGKGVATAFGAIAPIGWDLTGVMAGTWLLTVLLSGYSSLGAIVSALIAPFYVWWFKP
                     QFTFPVSMLSCLILLRHHDNIQRLWRRQETKIWTKFKRKREKDPE"
     gene            complement(67345..68277)
                     /gene="ttdR"
                     /locus_tag="PN977_000279"
                     /gene_synonym="dan"
     CDS             complement(67345..68277)
                     /gene="ttdR"
                     /locus_tag="PN977_000279"
                     /gene_synonym="dan"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator TtdR"
                     /protein_id="HDI6040362.1"
                     /translation="MLNSWPLAKDLQVLVEIVHSGSFSAAAATLGQTPAFVTKRIQIL
                     ENTLATTLLNRSARGVALTESGQRCYEHALEILTQYQRLVDDVTQIKTRPEGMIRIGC
                     SFGFGRSHIAPAITELMRNYPELQVHFELFDRQIDLVQDNIDLDIRINDEIPDYYIAH
                     LLTKNKRILCAAPEYLQKYPQPQSLQELSRHDCLVTKERDMTHGIWELGNGQEKKSVK
                     VSGHLSSNSGEIVLQWALEGKGIMLRSEWDVLPFLESGKLVRVLPEYAQSANIWAVYR
                     EPLYRSMKLRVCVEFLAAWCQQRLGKPDEGYQVM"
     gene            68484..69395
                     /gene="ttdA"
                     /locus_tag="PN977_000280"
     CDS             68484..69395
                     /gene="ttdA"
                     /locus_tag="PN977_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit alpha"
                     /protein_id="HDI6040363.1"
                     /translation="MMSESNKQQAVNKLTEIVANFTAMISTRMPDDVVDKLKQLKDAE
                     TSSMGKIIYHTMFDNMQKAIDLNRPACQDTGEIMFFVKVGSRFPLLGELQSILKQAVE
                     EATVKAPLRHNAVEIFDEVNTGKNTGSGVPWVTWDIIPDNDDAEIEVYMAGGGCTLPG
                     RSKVLMPSEGYEGVVKFVFENISTLAVNACPPVLVGVGIATSVETAAVLSRKAILRPI
                     GSRHPNPKAAELELRLEEGLNRLGIGPQGLTGNSSVMGVHIESAARHPSTIGVAVSTG
                     CWAHRRGTLLVHADLTFENLSHTRSAL"
     gene            69392..69997
                     /gene="ttdB"
                     /locus_tag="PN977_000281"
     CDS             69392..69997
                     /gene="ttdB"
                     /locus_tag="PN977_000281"
                     /EC_number="4.2.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit beta"
                     /protein_id="HDI6040364.1"
                     /translation="MKKILTTPIKAEDLQDIRVGDVIYLTGTLVTCRDVCHRRLIELK
                     RPIPYDLNGKAIFHAGPIVRKNGDKWEMVSVGPTTSMRMESFEREFIEQTGVKLVVGK
                     GGMGPLTEEGCQKFKALHVIFPAGCAVLAATQVEEIEEVHWTELGMPESLWVCRVKEF
                     GPLIVSIDTHGNNLIAENKKLFAERRDPIVEEICEHVHYIK"
     gene            70045..71508
                     /gene="ttdT"
                     /locus_tag="PN977_000282"
     CDS             70045..71508
                     /gene="ttdT"
                     /locus_tag="PN977_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-tartrate/succinate antiporter"
                     /protein_id="HDI6040365.1"
                     /translation="MKPSTEWWRYLAPLAVIAIIALIPVPAGLESHTWLYFAVFTGVI
                     VGLILEPVPGAVVAMVGISIIAILSPWLLFSPEQLAQPGFKFTAKSLSWAVSGFSNSV
                     IWLIFAAFMFGTGYEKTGLGRRIALILVKKMGHRTLFLGYAVMFSELILAPVTPSNSA
                     RGAGIIYPIIRNLPPLYQSQPNDSSSRSIGSYIMWMGIVADCVTSAIFLTAMAPNLLL
                     IGLMKSASHATLSWGDWFLGMLPLSILLVLLVPWLAYVLYPPVLKSGDQVPRWAETEL
                     QAMGPLCSREKRMLGLMVGALVLWIFGGDYIDAAMVGYSVVALMLLLRIISWDDIVSN
                     KAAWNVFFWLASLITLATGLNNTGFISWFGKLLAGSLSGYSPTIVMVALIVVFYLLRY
                     FFASATAYTSALAPMMIAAALAMPEIPLPVFCLMVGAAIGLGSILTPYATGPSPIYYG
                     SGYLPTVDYWRLGAIFGLIFLVLLVITGLLWMPVVLL"
     gene            complement(71551..72564)
                     /gene="tsaD"
                     /locus_tag="PN977_000283"
     CDS             complement(71551..72564)
                     /gene="tsaD"
                     /locus_tag="PN977_000283"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001264383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HDI6040366.1"
                     /translation="MRVLGIETSCDETGIAIYDDEKGLLANQLYSQVKLHADYGGVVP
                     ELASRDHVRKTVPLIQEALKESGLTAKDIDAVAYTAGPGLVGALLVGATVGRSLAFAW
                     DVPAIPVHHMEGHLLAPMLEDNPPAFPFVALLVSGGHTQLISVTGIGQYELLGESIDD
                     AAGEAFDKTAKLLGLDYPGGPLLSKMAAQGTAGRFVFPRPMTDRPGLDFSFSGLKTFA
                     ANTIRDNGTDDQTRADIARAFEDAVVDTLMIKCKRALDQTGFKRLVMAGGVSANRTLR
                     AKLAEMMKKRRGEVFYARPEFCTDNGAMIAYAGMVRFKAGATADLGVSVRPRWPLAEL
                     PAA"
     gene            72802..73017
                     /gene="rpsU"
                     /locus_tag="PN977_000284"
     CDS             72802..73017
                     /gene="rpsU"
                     /locus_tag="PN977_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HDI6040367.1"
                     /translation="MPVIKVRENEPFDVALRRFKRSCEKAGVLAEVRRREFYEKPTTE
                     RKRAKASAVKRHAKKLARENARRTRLY"
     gene            73128..74873
                     /gene="dnaG"
                     /locus_tag="PN977_000285"
     CDS             73128..74873
                     /gene="dnaG"
                     /locus_tag="PN977_000285"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HDI6040368.1"
                     /translation="MAGRIPRVFINDLLARTDIVDLIDARVKLKKQGKNFHACCPFHN
                     EKTPSFTVNGEKQFYHCFGCGAHGNAIDFLMNYDKLEFVETVEELAAMHNLEVPFEAG
                     SGPSQIERHQRQTLYQLMDGLNTFYQQSLQQPVATSARQYLEKRGLSHEVIARFAIGF
                     APPGWDNVLKRFGGNPENRQSLIDAGMLVTNDQGRSYDRFRERVMFPIRDKRGRVIGF
                     GGRVLGNDTPKYLNSPETDIFHKGRQLYGLYEAQQDNAEPNRLLVVEGYMDVVALAQY
                     GINYAVASLGTSTTADHIQLLFRATNNVICCYDGDRAGRDAAWRALETALPYMTDGRQ
                     LRFMFLPDGEDPDTLVRKEGKEAFEARMEQAMPLSAFLFNSLMPQVDLSTPDGRARLS
                     TLALPLISQVPGETLRIYLRQELGNKLGILDDSQLERLMPKAAESGVSRPVPQLKRTT
                     MRILIGLLVQNPELATLVPPLENLDENKLPGLGLFRELVNTCLSQPGLTTGQLLEHYR
                     GTNNAATLEKLSMWDDIADKNIAEQTFTDSLNHMFDSLLELRQEELIARERTHGLSNE
                     ERLELWTLNQELAKK"
     gene            75068..76909
                     /gene="rpoD"
                     /locus_tag="PN977_000286"
     CDS             75068..76909
                     /gene="rpoD"
                     /locus_tag="PN977_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HDI6040369.1"
                     /translation="MEQNPQSQLKLLVTRGKEQGYLTYAEVNDHLPEDIVDSDQIEDI
                     IQMINDMGIQVMEEAPDADDLMLAENTADEDAAEAAAQVLSSVESEIGRTTDPVRMYM
                     REMGTVELLTREGEIDIAKRIEDGINQVQCSVAEYPEAITYLLEQYDRVEAEEARLSD
                     LITGFVDPNAEEDLAPTATHVGSELSQEDLDDDEDEDEEDGDDDSADDDNSIDPELAR
                     EKFAELRAQYVVTRDTIKAKGRSHAAAQEEILKLSEVFKQFRLVPKQFDYLVNSMRVM
                     MDRVRTQERLIMKLCVEQCKMPKKNFITLFTGNETSDTWFNAAIAMNKPWSEKLHDVS
                     EEVHRALQKLQQIEEETGLTIEQVKDINRRMSIGEAKARRAKKEMVEANLRLVISIAK
                     KYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYKFSTYATWWIRQAITRSIADQARTI
                     RIPVHMIETINKLNRISRQMLQEMGREPTPEELAERMLMPEDKIRKVLKIAKEPISME
                     TPIGDDEDSHLGDFIEDTTLELPLDSATTESLRAATHDVLAGLTAREAKVLRMRFGID
                     MNTDHTLEEVGKQFDVTRERIRQIEAKALRKLRHPSRSEVLRSFLDD"
     gene            76968..77531
                     /locus_tag="PN977_000287"
     CDS             76968..77531
                     /locus_tag="PN977_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001183949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADAR family protein"
                     /protein_id="HDI6040370.1"
                     /translation="MQQGYYSLPALISALDAGKKVKYLWFWGHQPAANHEITASCFSQ
                     WWQGSPFTYDGITYATAEHWMMAGKARLFNDSKTLARILAAATPAEAKKLGRQVTNFD
                     EQCWQACCFDLVCEGNYHKFSQHSALKTFLLNTQPRILVEASPVDKIWGIGLAKDHPH
                     AGVPSHWKGKNLLGFALMVVRDRLAEE"
     gene            complement(77588..78094)
                     /gene="mug"
                     /locus_tag="PN977_000288"
     CDS             complement(77588..78094)
                     /gene="mug"
                     /locus_tag="PN977_000288"
                     /EC_number="3.2.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000228936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G/U mismatch-specific DNA glycosylase"
                     /protein_id="HDI6040371.1"
                     /translation="MVEDILAPGLRVVFCGINPGLSSAGTGFPFAHPANRFWKVIYQA
                     GFTDRQLKPQEAQHLLDYRCGVTKLVDRPTVQANEVSKQELHAGGRKLIEKIEDYQPQ
                     ALAILGKQAYEQGFSQRGAQWGKQTLTIGSTQIWVLPNPSGLSRVSLEKLVEAYRELD
                     QALVVRGL"
     gene            78219..78294
                     /locus_tag="PN977_000289"
     tRNA            78219..78294
                     /locus_tag="PN977_000289"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:78252..78254,aa:Ile,seq:cat)
     gene            complement(78348..79112)
                     /gene="nfeF"
                     /locus_tag="PN977_000290"
     CDS             complement(78348..79112)
                     /gene="nfeF"
                     /locus_tag="PN977_000290"
                     /EC_number="1.16.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent ferric chelate reductase"
                     /protein_id="HDI6040372.1"
                     /translation="MNNSPRYPQRVRNDLRFRELTVLRVERISAGFQRIVLGGEALDG
                     FTSRGFDDHSKLFFPQPDAHFVPPTVTEEGIVWPEGPRPPSRDYTPLYDELRHELAID
                     FFIHDGGVASGWAMQAKPGDKLTVAGPRGSLVVPEDYAYQLYVCDESGMPALRRRLET
                     LSKLAVKPQVSALVSVRDNACQDYLAHLDGFNIEWLAHDEQAVDARLAQMQIPADDYF
                     IWITGEGKVVKNLSRRFEAEQYDPQRVRAAAYWHAK"
     gene            79399..80022
                     /gene="nfeR"
                     /locus_tag="PN977_000291"
                     /gene_synonym="yqjI"
     CDS             79399..80022
                     /gene="nfeR"
                     /locus_tag="PN977_000291"
                     /gene_synonym="yqjI"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NfeR"
                     /protein_id="HDI6040373.1"
                     /translation="MSHHHEGCCKHEGQPRHEGCCKGEKSEHEHCGHGHQHEHGQCCG
                     GRHGRGGGRRQRFFGHGELRLVILDILSRDDSHGYELIKAIENLTQGNYTPSPGVIYP
                     TLDFLQEQSLITIREEEGGKKQIALTEQGAQWLEENREQVEMIEERIKSRCVGAALRQ
                     NPQMKRALDNFKAVLDLRVNQSDITDAQIKKIIAVIDRAAFDITQLD"
     gene            complement(80051..81571)
                     /gene="aer"
                     /locus_tag="PN977_000292"
     CDS             complement(80051..81571)
                     /gene="aer"
                     /locus_tag="PN977_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerotaxis sensor receptor Aer"
                     /protein_id="HDI6040374.1"
                     /translation="MSSHPYVTQQNTPLADDTTLMSTTDLQSYITHANDTFVQVSGFT
                     LQELQGQPHNMVRHPDMPKAAFADMWFTLKKGEPWSGIVKNRRKNGDHYWVRANAVPM
                     VREGKISGYMSIRTRATDEEIAAVEPLYKALNAGRTSKRIHKGLVVRKGWLGKLPSLP
                     LRWRARGVMTLMFILLAVMLWFVAAPVVTYILCALVVLLASACFEWQIVRPIENVARQ
                     ALKVATGERNSVEHLNRSDELGLTLRAVGQLGLMCRWLINDVSSQVSSVRNGSETLAK
                     GTDELNEHTQQTVDNVQQTVATMNQMAASVKQNSATASAADKLSITASNAAVQGGEAM
                     TTVIKTMDDIADSTQRIGTITSLINDIAFQTNILALNAAVEAARAGEQGKGFAVVAGE
                     VRHLASRSANAANDIRKLIDASADKVQSGSQQVHAAGRTMEDIVAQVKNVTQLIAQIS
                     HSTLEQADGLSSLTRAVDELNLITQKNAELVEESAQVSAMVKHRASRLEDAVTVLH"
     gene            81989..83368
                     /gene="ygjG"
                     /locus_tag="PN977_000293"
     CDS             81989..83368
                     /gene="ygjG"
                     /locus_tag="PN977_000293"
                     /EC_number="2.6.1.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417544.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine aminotransferase"
                     /protein_id="HDI6040375.1"
                     /translation="MNRLPSSASALACSAHALNLIEKRTLDHEEMKALNREVIEYFKE
                     HVNPGFLEYRKSVTAGGDYGAVEWQAGGLNTLVDTQGQEFIDCLGGFGIFNVGHRNPV
                     VVSAVQNQLAKQPLHSQELLDPLRAMLAKTLAALTPGKLKYSFFCNSGTESVEAALKL
                     AKAYQSPRGKFTFIATSGAFHGKSLGALSATAKSTFRKPFMPLLPGFRHVPFGNIEAM
                     RTALSECKKTGDDVAAVILEPIQGEGGVILPPPGYLTAVRKLCDEFGALMILDEVQTG
                     MGRTGKMFACEHENVQPDILCLAKALGGGVMPIGATIATEEVFSVLFDNPFLHTTTFG
                     GNPLACAAALATINVLLEQNLPAQAEQKGDMLLDGFRQLAREYPDLVQEARGKGMLMA
                     IEFVDNEIGYNFASEMFRQRVLVAGTLNNAKTIRIEPPLTLTIEQCEQVIKAARKALA
                     AIRVSVEEA"
     gene            complement(83410..83742)
                     /locus_tag="PN977_000294"
     CDS             complement(83410..83742)
                     /locus_tag="PN977_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-binding protein"
                     /protein_id="HDI6040376.1"
                     /translation="METVAYADFARLEMRVGKIVEVKRHENADKLYIVQVDVGEKTLQ
                     TVTSLVPYYSEEELMGKTVVVLCNLQKAKMRGETSECMLLCAETDDGSESVLLTPERM
                     MPAGVRIV"
     gene            83960..84943
                     /gene="ebgR"
                     /locus_tag="PN977_000295"
     CDS             83960..84943
                     /gene="ebgR"
                     /locus_tag="PN977_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator EbgR"
                     /protein_id="HDI6040377.1"
                     /translation="MATLKDIAIEAGVSLATVSRVLNDDPTLNVKEETKHRILEIAEK
                     LEYKTSSARKLQTGAVNQHHILAIYSYQQELEINDPYYLAIRHGIETQCEKLAIELTN
                     CYEHSGLPDIKNVTGILIVGKPTPALRAAACALTDNICFIDFHEPGSGYDAVDIDLAR
                     ISKEIIDFYINQGVNRIGFIGGEDLPGKADIREVAFVEYGRLKHVVREEDIWRGGFSS
                     SSGYELAKQMLAQEDYPKALFVASDSIAIGVLRAIHERGLNIPQDISLISVNDIPTAR
                     FTFPPLSTVRIHSEMMGSQGVNLLYEKARDGRALPLLVFVPSKLKLRGTTR"
     gene            85127..88219
                     /gene="ebgA"
                     /locus_tag="PN977_000296"
     CDS             85127..88219
                     /gene="ebgA"
                     /locus_tag="PN977_000296"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311985.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit alpha"
                     /protein_id="HDI6040378.1"
                     /translation="MNRWENIQLTHENRLAPRAYFFSYDSVAQARTFARETSSLFLSL
                     SGQWNFHFFDHPLQVPEAFTSELMADWGHITVPAMWQMEGHGKLQYTDEGFPFPIDVP
                     FVPSDNPTGAYQRIFTLSEGWQGKQTLIKFDGVETYFEVYVNGQYVGFSKGSRLTAEF
                     DISAMVKTGDNLLCVRVMQWADSTYVEDQDMWWSAGIFRDVYLIGKQLTHINDFTVRT
                     DFDEAYCDATLSCEVVLENLAASPVVTTLEYTLFDGERLVHSSAIDHLAIEKLTSASF
                     AFTVEQPQQWSAESPYLYHLVMTLKDADGNVLEVVPQRVGFRDIKVRDGLFWINNRYV
                     MLHGVNRHDNDHRKGRAVGMDRVEKDLQLMKQHNINSVRTAHYPNDPRFYELCDIYGL
                     FVMAETDVESHGFANVGDISRITDDPQWENVYVERIVRHIHAQKNHPSIIIWSLGNES
                     GYGCNIRAMYHAAKALDDTRLVHYEEDRDAEVVDIISTMYTRVPLMNEFGEYPHPKPR
                     IICEYAHAMGNGPGGLTEYQNVFYKHDCIQGHYVWEWCDHGIQAQDDNGNVWYKFGGD
                     YGDYPNNYNFCLDGLIYSDQTPGPGLKEYKQVIAPVKIHALDLTRGELKVENKLWFTT
                     LDDYTLHAEVRAEGETLATQQIKLRDVAPNSEAPLQITLPQLDAREAFLNITVTKDSR
                     TRYSEAGHSIATYQFPLKENTAQPVPFAPNNARPLTLEDDRLSCTVRGYNFAITFSKM
                     SGKPTSWQVNGESLLTREPKINFFKPMIDNHKQEYEGLWQPNHLQIMQEHLRDFAVEQ
                     SDDEVLIISRTVIAPPVFDFGMRCTYIWRIAADGQVNVALSGERYGDYPHIIPCIGFT
                     MGINGEYDQVAYYGRGPGENYADSQQANIIDIWRSTVDAMFENYPFPQNNGNRQHVRW
                     TALTNRHGNGLLVVPQRPINFSAWRYTQENIHAAQHCNELQRSDDITLNLDHQLLGLG
                     SNSWGSEVLDSWRVWFRDFSYGFTLLPVSGGEATAQSLASYEFGAGFFSTNLHSENKQ
                     "
     gene            88216..88665
                     /locus_tag="PN977_000297"
     CDS             88216..88665
                     /locus_tag="PN977_000297"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit beta"
                     /protein_id="HDI6040379.1"
                     /translation="MRIIDNLEQFRQIYASGKKWQRCVEAIENIDNIQPGVAHSIGDS
                     LTYRVETDSATDALFTGHRRYFEVHYYLQGQQKIEYAPKETLQVVEYYRDETDREYLK
                     GCGETVEVHEGQIVICDNHEAYRFICNNAVKKVVLKVTIEDGYFHNK"
     gene            88728..90161
                     /locus_tag="PN977_000298"
     CDS             88728..90161
                     /locus_tag="PN977_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDI6040380.1"
                     /translation="MSDTKRNTIGKFGLLSLTFAAVYSFNNVINNNIELGLASAPMFF
                     LATIFYFIPFCLIIAEFVSLNKNSEAGVYAWVKSSLGGRWAFITAYTYWFVNLFFFTS
                     LLPRVIAYASYAFLGYEYIMTPVATTIISMVLFAFSTWVSTNGAKMLGPITSVTSTLM
                     LLLTLSYILLAGTALVGGVQPADPITVDAMIPNFNWAFLGVTTWIFMAAGGAESVAVY
                     VNDVKGGSKSFVKVIILAGIFIGVLYSVSSVLINVFVSSKELKFTGGSVQVFHGMAAY
                     FGLPEALMNRFVGLVSFTAMFGSLLMWTATPVKIFFSEIPEGIFGKKTVELNENGVPA
                     RAAWIQFLIVIPLMIIPMLGSNTVQDLMNTIINMTAAASMLPPLFIMLAYLNLRAKLD
                     HLPRDFRMGSRRTGIIVVSMLIAIFAVGFVASTFPTGANILTIIFYNVGGIVIFLRFA
                     WWKYSKYIKGLTAEERHIEATPASNVD"
     gene            90295..91365
                     /gene="ygjJ"
                     /locus_tag="PN977_000299"
     CDS             90295..91365
                     /gene="ygjJ"
                     /locus_tag="PN977_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgjJ"
                     /protein_id="HDI6040381.1"
                     /translation="MKLITAPCRALLALPFCYAFSAAAEEARPAEHDDTKTPAITSTS
                     SPSFRFYGELGVGGYMDLEGENKHKYSDGTYIEGGLEMKYGSWFGLIYGEGWTVQADH
                     DGNAWVPDHSWGGFEGGINRFYGGYRTNDGTEIMLSLRQDSSLDDLQWWGDFTPDLGY
                     VIPNTRDIMTALKVQNLTGNFRYSVTATPAGHHDESKAWLHFGKYDRYDDKYTYPAMM
                     NGYIQYDLAEGITWMNGLEITDGTGQLYLTGLLTPNFAARAWHHTGRADGLDVPGSES
                     GMMVSAMYEALKGVYLSTAYTYAKHRPDHADDETTSFMQFGIWYEYGGGRFATAFDSR
                     FYMKNASHDPSDQLFLMQYFYW"
     gene            91382..93733
                     /gene="ygjK"
                     /locus_tag="PN977_000300"
     CDS             91382..93733
                     /gene="ygjK"
                     /locus_tag="PN977_000300"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HDI6040382.1"
                     /translation="MKIKTILTPVTCALLISFSAHAANADNYKNVINRTGAPQYMKDY
                     DYDDHQRFNPFFDLGAWHGHLLPDGPNTMGGFPGVALLTEEYINFMASNFDRLTVWQD
                     GKKVDFTLEAYSIPGALVQKLTAKDVQVEMTLRFATPRTSLLETKITSDKPLDLVWDG
                     ELLEKLEAKEGKPLSDKTIAGEYPDYQRKISATRDGLKVTFGKVRATWDLLTSGESEY
                     QVHKSLPVQTEINGNRFTSKAHINGSTTLYTTYSHLLTAQEVSKEQMQIRDILARPAF
                     YLTASQQRWEEYLKKGLTNPDATPEQTRVAVKAIETLNGNWRSPGGAVKFNTVTPSVT
                     GRWFSGNQTWPWDTWKQAFAMAHFNPDIAKENIRAVFSWQIQPGDTVRPQDVGFVSDL
                     IAWNLSPERGGDGGNWNERNTKPSLAAWSVMEVYNVTQDKTWLAEMYPKLVAYHDWWL
                     RNRDHNGNGVPEYGATRDKAHNTKSGEMLFTVKKGDKEETLSGLNNYARVVEKGQYDS
                     LEIPAQVAASWESGRDDAAVFGFIDKEQLDKYVANGGKRSDWTVKFAENRSQDGTLLG
                     YSLLQESVDQASYMYSDNHYLAEMATILGKPEEAKRYRQLAQQLADYINTCMFDPTTQ
                     FYYDVRIEDKPLANGCAGKPIVERGKGPEGWSPLFNGAATQANADAVVKVMLDPKEFN
                     TFVPLGTAALTNPAFGADIYWRGRVWVDQFWFGLKGMERYGYRDDALKLADTFFQHAK
                     GLTADGPIQENYNPLTGAQQGAPNFSWSAAHLYMLYNDFFRKQ"
     gene            93904..95922
                     /gene="fadH"
                     /locus_tag="PN977_000301"
     CDS             93904..95922
                     /gene="fadH"
                     /locus_tag="PN977_000301"
                     /EC_number="1.3.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 2,4-dienoyl-CoA reductase"
                     /protein_id="HDI6040383.1"
                     /translation="MSYPSLFASLDLGFTTLKNRVLMGSMHTGLEEYPDGAERLAAFY
                     AERARHGVALIVSGGIAPDLTGVGMEGGAMLNDASQIPHHRTITEAVHQEGGKIALQI
                     LHTGRYSYQPHLVAPSALQAPINRFVPHELSHEEILQLIDNFARCAQLAREAGYDGVE
                     VMGSEGYLINEFLTLRTNQRSDQWGGDYHNRMRFAVEVVSAVRERVGNDFIIIYRLSM
                     LDLVEDGGTFAETVELAQAIEAAGATIINTGIGWHEARIPTIATPVPRGAFSWVTRKL
                     KGHVSLPLVTTNRINDPQVADDILARGDADMVSMARPFLADAELLSKAQSGRADEINT
                     CIGCNQACLDQIFVGKVTSCLVNPRACHETKMPILPAVQKKNLAVVGAGPAGLAFAIN
                     AAARGHQVTLFDAHSEIGGQFNIAKQIPGKEEFYETLRYYRRMIEVTGVTLKLNHTVT
                     ADQLKAFDETILASGIVPRIPPIDGIDHPKVLSYLDVLRDKAPVGNKVAIIGCGGIGF
                     DTAMYLSQPGESTSQNIAGFCNEWGIDSSLQQAGGLSPQGMQIPRSPRQIVMLQRKAS
                     KPGQGLGKTTGWIHSTTLLSRGVKMIPGVSYQKIDDDGLHVVINGETQVLAVDNVVIC
                     AGQEPNRALAQPLIDSGKTVHLIGGCDVAMELDARRAIAQGTRLALEI"
BASE COUNT        24203 a        25161 c        23915 g        22810 t
ORIGIN      
        1 ctgattatct ctaccaaggc tggctacgat atgtggcccg gcccttacgg ctctggcggt
       61 tcacgtaaat acctgctcgc cagcctcgac caaagcctga agcggatggg gcttgagtat
      121 gtcgatatct tttactctca tcgcgtcgat gaaaatacgc cgatggaaga aaccgcctct
      181 gcgctggctc atgcggtaca aagcggtaag gcgctgtatg tcgggatctc ctcttactcg
      241 ccagagcgga cgcaaaaaat ggtcgagtta ctgcgcgagt ggaaaattcc gctgttaatt
      301 catcaacctt cgtacaattt actgaaccgc tgggtggata aaagcggcct gctggatacc
      361 ctgcaaaata acggcgtggg ctgcattgcc tttactcctc tggctcaggg attgctgacc
      421 ggaaaatatc tcaacggcat tccgcaagat tcacggatgc atcgtgaagg gaataaagtt
      481 cgtggtctga cgccgaaaat gctcaccgaa gccaacctca acagcctgcg gttattgaat
      541 gaaatggcac agcagcgtgg acaatcaatg gcgcaaatgg cgttaagctg gttgctgaaa
      601 gatgatcgcg tgacgtcggt attgattggt gccagccgcg cggagcaact tgaggagaac
      661 gtgcaggcgc tgaataatct gacatttagc accgaggagc tggcgcagat tgatcagcat
      721 atcgccgatg gcgagctgaa tctgtggcag gcgtcttccg ataaatgacc tgttaataac
      781 gggcgcgaga accgcgcccg aatcagatca gtgattatga cgagtcagtc ggtcaagata
      841 gcccatcaca aatgcagaga gcacaaacgt cagatggata atgacgtacc acatcagttt
      901 gttatcaggg acatttttcg catccataaa gacgcgcagt aagtgaatgg aagaaattgc
      961 cacaatcgac gctgctactt tgtttttcag cgacgttgcg tccattttcc ccagccagtt
     1021 cagcttctct ttgttctcgg agatatccag ttgtgagacg aaattctcat aaccggaaaa
     1081 catcaccatc accagcaaac cgccaaccag cgtcatatcc accagcgaca gcaacacgag
     1141 gatcaaatct gattccgcca tcgagaagat attcggcagt acgtgaataa tctcctggaa
     1201 gaacttcagc gccagggcaa ctaacgccag cgaaaggcca aagtacacgg gggcaagcag
     1261 ccagcgagaa gcatacattg cattttcaag aaaacgttcc atacattcct gtcgtgtagt
     1321 taaaacgggc aacagtatat cgcaattgag taaacagcgc gcaacagcaa atcaacatat
     1381 ccgttaaacc tttgtcgcat aaatccgagg tttagggatc ttcctggttc ccttatcaac
     1441 gcatcgagag cgtctaccct taaaggacgt tgaacctgaa gggagaaaaa caatgtctca
     1501 tttaaaagac ccgaccacgc agtattacac tggtgaatat cccaaacaga aacaaccgac
     1561 gccaggcatc caggcgaaga tgacaccggt accggattgc ggcgagaaaa tctatgttgg
     1621 tagcggtcgc ctgaaagatc gtaaggcact ggtgacaggg ggcgattccg gaatcggtcg
     1681 cgctgccgcc atcgcttacg cgcgtgaagg ggctgacgtg gcgatcagtt atcttcccgt
     1741 ggaagaagaa gacgctcagg atgtgaaaaa gatcattgaa gaatgcggac gcaaagccgt
     1801 tctgctgcca ggcgatttaa gcgatgagaa atttgcccgt tcgctggttc acgaagcgca
     1861 taaggcctta ggcgggctgg atattatggc gctggtcgcc gggaaacagg ttgccattcc
     1921 tgatattgca gacctcacca gcgaacagtt tcaaaagacc tttgccatta acgttttcgc
     1981 gctattctgg ctaacccagg aagcgatccc cctgctaccg aaaggtgcaa gtattatcac
     2041 cacttcgtca atccaggcat accagccaag cccgcattta ctggactatg cggctacgaa
     2101 ggcggcgatt ctgaactaca gccgaggcct ggcaaaacag ctcgcggaga aaggtattcg
     2161 ggtgaatatt gtcgcgccag gcccgatctg gacagcactg caaatttccg gcgggcaaac
     2221 gcaggataag atcccgcagt ttggtcagca aacgccgatg aaacgtgcgg ggcaaccggc
     2281 ggaactggcc cctgtatatg tttatctggc aagtcaggaa tcgagctacg tcaccgcaga
     2341 agtgcacggc gtgtgcggcg gcgagcattt aggttaaaaa atgcccggtt gtgaaaagca
     2401 accgggcatc atttttattt cgctttggca gtttcttcgc caaccagacc tatcttcagg
     2461 taacccgcct gatgcagcgt atccattacc ttcatcaacg tctcgtaatc gacggttttg
     2521 tccgctcgga agaagatggt ggtgtctttc ttgccttcgg ttaacgcatt caacgccgta
     2581 atcattgttt catcggtgac cggatcgtta ccgataaaca tcgagttgtc tgccttcacc
     2641 gacagataaa ccggtttttc cgggcgcggc tgcggcgtgc tggtagaagc aggcaagttc
     2701 accttcacat ctaccgtcgc taacggtgcc gccaccataa agataatcag cagaaccaac
     2761 atcacgtcga taaacggcgt cacgttgata tcatgcattt cgccgttatc gtcgaggttt
     2821 tcgttaagat gcattgccat cggacattat cctgcgcgta atttttgtgc gacacgaacc
     2881 ggatgcgcag cggcgctggc ttccagatcc aggtcacggc tttgcagcaa caatacctgc
     2941 gctgcaacat cacccagcat cgctttaaag ccgccaatct ggcgtgcaaa tacgttatag
     3001 ataacgaccg ccggaatcgc tgccacgagg ccgattgccg ttgctaacag agcttctgcg
     3061 atacccggcg caacgactgc caggttagtg gtctgcgttt gcgcgatacc aataaagctg
     3121 ttcatgatgc cccagaccgt accaaacaga ccaacgaacg gcgaaatcgc gccgatggtt
     3181 gccagatagc cgttaccgcg gcccatttga cgacctactg cggcgacccg acgctccaga
     3241 cggaagctgg tacgttcttt aataccttcg ttatcgtcgc tgccttctga cagttccagc
     3301 tcattctgcg cttcattgag caaatgcagg cttaagcttt tgctaccaaa atcagcggcg
     3361 atatcgttgg cctggtttaa ggaacgcgct tcagccagca gttgctgctc gcgcttaagg
     3421 cgacgcttct gattgaagaa ctctacgctc ttactgaaga agattgccca ggtgactacg
     3481 gaggccaaaa taagcccaat catcacgcac ttaacgacaa tatcggcgtg ctgatacata
     3541 ccccagacgg aaaggtccgt ctgcattaaa ttattaccca ctgtgtatct ccaggacgca
     3601 agtcacaaaa tctgcgcata ataatatcaa aacgacgtcg aattgatagt cgttctcatt
     3661 actatttgca tactgccgta cctttgcgtt cttttccttg cgtttacgca gtaaaaaagt
     3721 caccagcacg ccatttgcga aaattttctg ctttatgcca attcttcagg atgcgcccgc
     3781 gaatattcat gctagtttag acatccagac gtataaaaac aggaatcccg acatggcgga
     3841 caaaaagctt gatactcaac tggtgaatgc aggacgcagc aaaaaataca ctctcggcgc
     3901 ggtaaatagc gtgattcagc gcgcttcttc gctggtcttt gacagtgtgg aagccaaaaa
     3961 gcacgcgaca cgcaatcgcg ccaatggcga gttgttctat gggcggcgcg gaacgttaac
     4021 ccatttttcc ttacaacaag cgatgtgtga actggaaggt ggcgcaggct gcgcgctatt
     4081 tccctgcggg gcggcggcgg ttgctaattc gattcttgct tttgtcgaac agggcgatca
     4141 tgtgttgatg accaacaccg cctatgaacc gagtcaggat ttctgtagca aaatcctcag
     4201 caaactgggc gtaacgacat cgtggtttga tccactgatt ggtgccgata tcgttaagca
     4261 tctgcagcca aacactaaaa tcgtgtttct ggaatcgcca ggctccatca ccatggaagt
     4321 ccacgacgtt ccggcgattg ttgccgccgt acgcagtgtg gtgccggatg ccatcattat
     4381 gatcgacaac acctgggcag ccggtgtgct gtttaaggcg ctggattttg gcattgatgt
     4441 ttccattcag gccgctacca aatatctggt tgggcattca gatgcgatga ttggcactgc
     4501 ggtgtgcaat gcccgttgct gggagcagct gcgagagaat gcctatctga tgggccagat
     4561 ggtcgatgcc gataccgcct atataaccag ccgtggcctg cgcactttgg gtgttcgcct
     4621 gcgtcaacat catgaaagca gtctgaaagt ggctgaatgg ctggcagaac atccccaagt
     4681 agcgcgagtt aaccaccctg ctctgcctgg cagtaaaggc cacgaattct ggaaacgaga
     4741 ctttacaggt agcagcgggc tattttcctt tgtgcttaag aaaaagctca atgatgaaga
     4801 actggcgaac tatctggata acttcagttt attcagcatg gcctactcgt ggggcgggta
     4861 tgaatcgttg atcctggcaa atcaaccaga acatatcgcc gccattcgcc cacaaggcga
     4921 gatcgatttt agcgggacct tgattcgcct gcatattggt ctggaagatg tcgacgatct
     4981 gattgccgat ctggatgccg gttttgcgcg aattgtataa cattgccact tttggacaat
     5041 tttgcagaca ttttattgtg aaaagtctta aattgttgcg tccgggatca aggcgtcccg
     5101 gacgattcag gagtacaata ggcagataaa ggcttaaacg ctgttccaca ggaaagtcca
     5161 tggctgttat tcaagatatc atcgctgcgc tctggcaaca cgactttgcc gcgctggcgg
     5221 atcctcatat tgttagcgtt gtttactttg tcatgtttgc cacgctgttt ttagaaaacg
     5281 gcctgctgcc cgcctcattt ttgccaggcg acagcttgtt gatattggct ggcgcattga
     5341 ttgcccaggg ggttatggat tttctgccta cgattgcgat tctgaccgcc gcagcaagtc
     5401 tgggctgctg gctaagttat attcaggggc gctggttagg aaataccaaa acggtgaaag
     5461 gctggctggc acagcttcct gctaaatatc accagcgcgc cacctgcatg tttgaccgcc
     5521 acggtctgct ggcgctgctg gctggacgtt ttcttgcatt tgtccgtacg ctgctgccaa
     5581 ccatggcagg tatttccggt ttgccaaacc gccgtttcca gtttttcaac tggttaagcg
     5641 gattgctgtg ggtcagcgtg gtaaccagtt tcggctatgc attaagtatg attccgtttg
     5701 ttaaacgcca tgaagatcag gtaatgacgt tcctgatgat cctgccaatt gccttgttaa
     5761 ccgctggctt gttgggcacg ctgtttgtgg tgattaaaaa aaaatactgt aacgcctgac
     5821 gattttcccc gttcccggtt gctgtaccgg gaacgtattt aattcccctg catcgcccgc
     5881 attcttgccg catcttcccc cggcgtcaca ccgaagtaac gtttaaactc acggctaaat
     5941 tgcgatgcgc tttcgtaacc gacgcgcatc gctgccgcac tggctttcat gccgtcgtgg
     6001 atgatcatca tccgcgcctt atgcagacgg taattcttca aatactgcaa cggcgaggtg
     6061 ctggtgacag acttaaaatt atggtggaac gccgatacgc tcatgttggc ttctgccgcc
     6121 agttgctcga cgctcaggtt ttcggtgtat ttattctcaa tccgtttcag cacgcggcta
     6181 ataaggctga agtgagtctg gcgactgacc agcgccagta acgcgccgcc gcaaggtccg
     6241 gtcagcacgt agtacagaat ttcgcggatg atctgtttgc cgagaatacg cgcatccagc
     6301 ggtcgctcca tcacgtcgag taaccgctcc gccgcgcata aaatctcttc tgataacgtg
     6361 gcggagttaa tcccgctggc tgccatcgac ggctggaaat gctcatcttc gccaatgtcc
     6421 atcaacagtt cctgtaactg caaaatatcg acattgagac gcaaccctgc cagcggcacc
     6481 tctgacgtgg cataggtttc gcactcaaac ggcaacggca ccgtcagcag caggtattca
     6541 ttggcatcat aacgaaacac gcgttcattg atataaccga ttttatgtcc ggaaaagaga
     6601 attatgatgc caggctcgta catcaccggt gtgcgtgcga aaggcgtctc gccatacaac
     6661 aaacgcacat cgggcaacag ctctgacaaa ctattttctt tatttttcag tttattaact
     6721 ttatccgcca gcaagcggca aatctcttca cgtttcatat cgcgtaattt cttaggaata
     6781 atgcggcaat ttgattgtgc gcaattttgt agcatttctc cagcactctg gagaaatagg
     6841 caagacattg gcagaaatga gcattgagag gtacggcgct ggcgaccaca atgaaaaaca
     6901 tcaggcagac cgatctctgc cctcatattg acccagcaaa gggagcaagt aatgaacaac
     6961 tttaatctgc acaccccaac ccgcattctg tttggtaaag gcgcaatcgc tggtttacgc
     7021 gagcaaattc ctcacgatgc acgcgtattg attacctacg gcggcggcag cgtgaaaaaa
     7081 accggcgttc tcgatcaagt tctggatgcc ctgaaaggca tggacgtgct ggaatttggc
     7141 ggtattgagc caaacccggc ttatgaaacg ctgatgaacg ccgtgaaact ggttcgcgaa
     7201 caaaaagtga ctttcctgct ggcggttggc ggcggttctg tactggacgg caccaaattt
     7261 atcgccgcag cggctaatta tccggaaaat atcgatccgt ggcacattct gcaaacgggc
     7321 ggcaaagaga ttaaaagcgc catcccgatg ggctgtgtgc tgacgctgcc agcaaccggt
     7381 tcagaatcca acgcaggcgc ggtgatctcc cgtaaaacca caggcgacaa gcaggcattc
     7441 cattccgccc atgttcagcc ggtatttgcc gtgctcgatc cggtttatac ctacaccctg
     7501 ccgccgcgtc aggtggctaa cggtgtagtg gatgcctttg tgcacaccgt ggaacagtat
     7561 gttaccaaac cggttgatgc caaaattcag gaccgtttcg cagaaggcat tttgttgacg
     7621 ctgatcgaag atggtccgaa agccctgaaa gagccagaaa actacgatgt gcgcgccaac
     7681 gttatgtggg cggcgactca ggcgctgaac ggtttgatcg gcgctggcgt accgcaggac
     7741 tgggcaacgc atatgctagg ccacgaactg actgcgatgc acggtctgga tcacgcacaa
     7801 acactggcta tcgtcctgcc tgcactgtgg aatgaaaaac gcgataccaa gcgcgctaag
     7861 ctgctgcaat atgctgaacg cgtctggaac atcactgaag gttccgacga tgagcgtatt
     7921 gacgccgcga ttgccgcaac ccgcaatttc tttgagcaat taggcgtgcc gacccacctc
     7981 tccgactacg gtctggacgg cagctccatc ccggctttgt tgaaaaaact ggaagagcac
     8041 ggtatgaccc aactgggcga aaatcatgac attacgctgg atgtcagccg ccgcatatac
     8101 gaagccgccc gctaagcttt ttacgcctca aactttcgtt ttcgggcatt tcgtccagac
     8161 ttaagttcac aacacctcac cggagcctgc tccggtgagt tcatataaag gaggaacgta
     8221 tggctaatcc aaccgttatt aagctacagg atggcaatgt catgccccag ttgggactgg
     8281 gcgtctggca agcaagtaat gaggaagtaa tcaccgccat tcaaaaagcg ttagacgtgg
     8341 gttatcgctc gattgatacc gccgcggcct acaagaacga agaaggtgtc ggcaaagccc
     8401 tgaaaaatgc ctcagtcaac agagaagaac tgttcatcac cactaagctg tggaacgacg
     8461 accacaagcg cccccgcgaa gccctgctcg acagcctgaa aaaactccag cttgattata
     8521 tcgacctcta tttaatgcac tggcccgttc ccgctatcga ccattatgtc gaagcatgga
     8581 agggcatgat cgaattgcaa aaagagggat taatcaaaag cattggcgtg tgcaacttcc
     8641 agatccatca cctgcaacgc ctgattgatg aaactggcgt gacgcctgtt attaatcaga
     8701 tcgaacttca cccgctgatg caacaacgcc agctacatgc ctggaacgcg acacacaaaa
     8761 tccagaccga atcctggagc ccattagcgc aaggagggaa aggcgttttc gatcagaaag
     8821 tcattcgcga tctggcagat aaatacggca aaaccccggc gcagattgtt atccgctggc
     8881 atctggatag cggcctggtg gtgatcccga aatcggtcac accttcacgt attgccgaaa
     8941 actttgatgt ctgggatttc cgtctcgaca aagacgaact cggcgaaatt gcaaaactcg
     9001 atcagggcaa gcgtctcggt cccgatcctg accagtttgg cggctaacgg gcaaattctc
     9061 ccggtggcgg taatgttccg ctaccggact tttcagaaat catttattcc cctcgcgtct
     9121 tgcccattgt tactcttcct tgttcaggaa tgccaaatat aaggacatca tcatgcagag
     9181 ccggaagctc ttaaaggaac aactcatcta cacccgggat aaacgcaacg gagaggtgaa
     9241 aaacagatga aaataatact tctgttttta gcagccctgg caagttttac cgtacaggcg
     9301 cagccttctt cacagaccgt agaacaaaca gtccggcaga tttatcagaa ctataaatca
     9361 gatgccagta cgccttattt tggtgaaacc ggagagcgag cgataacttc tgcgcgtatt
     9421 caacaggcgc ttaccctgaa cgacaatctt acactaccgg gcaatattgg ctggctggat
     9481 tatgatccgg tttgtgattg tcaggatttt ggcgatctgg tgttagaaag cgtagcgata
     9541 acccaacctg acgccgatca tgccgatgcc gttgtgcgct ttcgtatctt taaagacgat
     9601 aaagaaaaga ccacgcagac actgaaaatg gtggcggaaa atggtcgttg ggtcattgac
     9661 gatattgtca gcaatcatgg cagcgtctta caagcggtaa atagcgagaa tgaaaaaacg
     9721 ctggccgcta tagcttcgtt gcaaaaagaa cagccggaag cctttgttgc cgaactcttt
     9781 gaacatattg ctgattatag ctggccgtgg acgtgggtgg tttccgactc ttaccgccag
     9841 gcggtcaatg ccttctataa aaccaccttc aagactacca acaatcccga tgaagatatg
     9901 caaatagaac ggcaatttat ttacgacaat ccgatctgtt ttggcgaaga gtcgctattt
     9961 tcacgcgttg atgaaatccg tgtcctggag aaaaccgccg attccgcccg cattcatgtt
    10021 cgttttacgc tgagcaatgg caacaacgaa gagcaagaac tggttttaca gcggcgcgaa
    10081 ggtaagtggg aaatcgctga ttttatccgc ccgaatagcg gcagcctact taagcagatt
    10141 gaggcaaaaa ccgccgccag attaaagcaa tgagctgaat taaataacaa ttagccggaa
    10201 caataaataa aagggaacac tatatgaaaa cgattttcac cgtgggagct gttgttctgg
    10261 caacctgctt gctcagtggc tgcgtcaatg agcaaaaggt caatcagctg gcgagcaatg
    10321 tgcaaacatt aaatgccaaa atcgcccggc ttgagcagga tatgaaagca ttacgcccac
    10381 aaatctatgc tgccaaatcc gaagctaaca gagccaatac gcgtcttgat gcacaggact
    10441 atttcgactg cctgcgctgc ttgcgtatgt acgcagaatg ataaaaaaat ccccggcagc
    10501 attctgttac cggggatatt ttttaacgtc caaccgccgc tttagggcgt ttcttcgcac
    10561 cagcattctg caggcgagat tgcgtagacg acgctttttt tgccgtagca ggcgtctgac
    10621 gctgggtcgc catcggcgta tgtttagtca acgccggacg ggtattgcgg ttctggcggc
    10681 gagcttcacg catctcttca atggttggcg caggcactaa gcaatcgcga cggctgccaa
    10741 tcagatgctt tttgcccatc gcttccagcg cctgacggat taacggccag tttgccggat
    10801 cgtggtaacg caacaacgct ttatgcaaac gacgctgttt gtcgcccttc ggtacgaaga
    10861 cgtcttcact cttataacca atcttcgcca gcgggttttt cccggtgtaa tacatggtgg
    10921 tcgagttagc cagcggcgat gggtagaagt tctgtacctg gtcgagacgg aaacgatgct
    10981 ttttcagcca cagcgccaga ttcaccatat cttcatcacg cgtacccggg tgcgcggaga
    11041 tgaaatacgg gatcagatac tgttctttac ctgcctgttt cgagtaagta tcgaacagct
    11101 ctttaaagcg gtcatagctg cccatgcccg gcttcatcat cttcgataac ggcccttctt
    11161 cggtatgttc cggggcaatc ttcagataac cgccgacgtg atgggtcgcc agctctttga
    11221 tatagcgcgg atcttctacg gcgatgtcat aacgcacacc agaggcgatg aggatctttt
    11281 taatgccttt cagatcacga gcgcggcgat agaggttgat ggtcggttca tggttagtgt
    11341 ccatgtgcgg acaaatatcc ggataaacgc acgacaaacg acggcaggtt tgctcagcgc
    11401 gtggcgattt gcagcgcaac atatacatgt tggcagtagg cccaccgaga tcggaaatca
    11461 cgcccgtaaa acctggaaca gtgtcgcgga tcgcttcgat ctcattaata atcgaatctt
    11521 cggaacggct ctgaataatg cgcccttcgt gctcggtaat agaacagaaa gagcagccgc
    11581 caaagcagcc acgcataatg ttgaccgaga aacgaatcat ttcgtaagcc ggaattcggg
    11641 cattaccgta agccggatgc ggcacgcgct tgtacggcag cgcaaagacg ctatccatct
    11701 cttcggtaga aagcggaata gcaggcgggt tgatccatac atagcggtcg ccatgttttt
    11761 gcatcaatgc gcgggcgcag cctgggttag tttcgtggtg cagaatgcgc gaagcatggg
    11821 cgtacagcac tttatcgccc ttcactttct cgaaagaagg cagcaacacg taggtttttt
    11881 cccacggttt cgggcgtggt ggctgcacgg ttacagcttt ggcttcctgc tttttcggag
    11941 ctaccggttt gttatccgcg cacggcaaat cttcgccata cggatgcggg attgggtcga
    12001 ttttccctgg ggtatcaaga cgggtggaat ccacgccgct ccagcctggc aacgcctctt
    12061 ttacgataat cgcggtatta cgcacatcgc ggatttcact aatcggctcg cccatcgcca
    12121 gacggtgcgc cacttccacc agcggacgct caccgttacc aaacatcagc atgtcggctt
    12181 tcgaatccac caacacggaa cggcgcacgg tatcggacca gtaatcataa tgcgcggtac
    12241 ggcgcaggct ggcctcaata ccgccgagga tcaccggcac atctttccac gcctctttac
    12301 aacgttgggt ataaaccagt gtggcgcgat ccggacgctt acccgcgaca ttatctggcg
    12361 tgtaggcatc gtcatgacgt aaacggcgat cggcggtata gcggttgatc atcgagtcca
    12421 tgttgccagc agtaacgccg aaaaacagat tcggtttacc cagacgcata aagtcgtctt
    12481 tgctgctcca gtctggctgg gcgatgatcc cgacgcgaaa gccctgcgct tccagcatac
    12541 gaccgcaaat caccatcccg aagcttgggt gatcgacata cgcgtcgcca gtaaccaaaa
    12601 tgatgtcgca gctatcccag ccaagttgat ccatctcttc acgtgacatc ggcaaaaacg
    12661 gtgccggtcc aaaacaggcg gcccagtact gcggccagga gaacaggtcg cgatccggtt
    12721 ggatcaggga gatagagctc attttgcttc cagaaatgat aaaaaaataa tcaaaggccg
    12781 gggattatac gctgtatcac gcgcggaaat gaagtgatgt tgatgaacgg tggcgaaata
    12841 ccctggtgag gtttttcact gcccgacttg ggcaagcaca tttaaggctg gcggtatcgt
    12901 ggcaactgcc gctactattt atgccgatca gcaacgttca aatcatattg cacgacgatc
    12961 ggctatccga ggacacccac agtaaggcaa ggtatggaac aggttattac aaagcgccgc
    13021 tattacgata ttggtctaca gattgaagag ctgctgtatt caggcgtatt taaggccgga
    13081 gaacgcttac cttcagaacg tgaactgagc gagcggttta ataccagccg aactaccatt
    13141 cgtgaagcca tcattatgct ggaactgaaa ggcgtattga atgtgaagca agggtcaggg
    13201 attttctttg tcgactccac cgataagctc aaccagaaat cattaatgcc ctattcagaa
    13261 attggcccgt ttgaactgtt gcaggcgcgt caggtgattg agagcaatat tactggtttt
    13321 gcagccagcc agattagctt taacgagtta caagagttaa agaaaatcat tagattgcag
    13381 gaaaacgcta tcgctgcgga aagtgataaa tttgaagacc tggatcaccg atttcatagc
    13441 attattgccg aagcgacgca aaatcgggta ctcatcaaac aagctgctga attatggcgg
    13501 gcagttcgta cagaaaaccc ccgctggaag aaacttaact acaagtacct gcatgaaaag
    13561 catttgcgtc tgcaatggct ggaagatcac cgggctattt tccttgcgct acaacagaaa
    13621 gattcagagc tcgccaggga agcatcctgg agacatctgg aaaacagcaa aaatgaactg
    13681 atcaaaatct tcaaacaaga cgcttccatc agcgattttg atgacttttt cttcgctcgt
    13741 taaccttcat tagtccagag ctggcgggaa aaataacgcc ggctctgcct tccgccctcc
    13801 actggtacac gctctctttt caattggtct aataaatcga ctatcatcag atatcttaga
    13861 ttagcggcaa ttatcccaaa aactgacctc taaaaaactt tcctttcatt ttattcatat
    13921 aactcattga tacagatata aataacgcct tgttgttctg tttcgtactt ttacctttct
    13981 ctgcaagcgt gatgcatctc tctgtttttg gtctagtgaa ttatgtaaat tggtatacac
    14041 acaagccacc aggagtaaga gcataatgtc agggaacgaa agtatcgctg ccgcccacta
    14101 tgatcaggta accacctggc ctcgggacgg gttacaggca gatattgtcc atattggttt
    14161 tggcgctttt catcgcggac accaggctgt ctacacggat ctcactaatc aactttcgga
    14221 cacccgctgg gggatctttg agatcaacct gtttggtgat gctcaactga tcgaaaactt
    14281 aaatgcgcaa aatgggctgt tttcggttgt ggaaacatct gcatcgcaat ctacctcacg
    14341 ccttgtgcgt tcggtggctg gcggtattca tacccccaga gatggcattg ccgcagcaat
    14401 ccataaactg gctgaacctc aggtaaaaat cgtttcatta accatcaccg agaaaggtta
    14461 ttgcctcgat ccgcaaacac gttcacttga tctcaccaat ggattaatcc aacacgatct
    14521 acaaaacccg gatgcgcctc tgtcagctat tggcgtgatc gtctgtgctt tgcaacaacg
    14581 taaagcgaca ggactcgccg cttttagtgt gctctcctgt gacaacttgc cagacaatgg
    14641 gcatctgacg cgcaatgccg ttctcggttt tgcccgacaa ctggaccagc ccctggctca
    14701 gtggatcgaa gaaaatgtct catttccagg cacgatggta gatcgcattg ttccggcaat
    14761 gactgaatcg caattcgcct tactggaaac gaaaaccggt tatgccgatc cctgcgggat
    14821 cgtctgcgaa tcatttcgtc agtgggtgat cgaagataat tttgtgcgcg gacgaccaca
    14881 atgggataaa gccggcgcga tgtttgtcag caatgttcag ccttatgaag agatgaagtt
    14941 acgcatgtta aatggtagcc attcatttct ggcttataac ggctcgctgg caggctatga
    15001 gtttatctgg caatgcatgg aagacgctaa ttttcgttcc attacccacc aactgatgat
    15061 taatgaacaa gcccgaacac ttaatccaga cttaaatatc aacatccagg aatacgccga
    15121 cctgttaatt gaacgcttta gcaaccgtaa cgttgcacac cgtaccgggc aaatagccat
    15181 ggacggttca caaaagcttc cccagcgagc gctgacgccc tggctgaaat tgcatcagca
    15241 aaagcaaaac aatgctgttc tgtcactgct cattgctggt tggttgcatt atgtcattga
    15301 tgctgttgag aaaagccagt ctgccgctga tccaatgaat gaccaatttc aggcgctaat
    15361 aaaggaacaa caagacgcat ggcaacaggc gctcgcatta ctgcacctta gcgccatatt
    15421 tggtgattta agcaaccatc agccatttat aaatgaaata aaaatcgcct ttgcgaatat
    15481 aaaaaacaaa ggcatcaagg ccaccatcag ccaattatta tcggatgagc agaaatgaaa
    15541 acattaattt gtcagcagcc tggcgttatg gaatatgtgg aaaaggatat tcccacacca
    15601 gcagataatg aagtgctgtt aaaaatcaaa gctgtgggta tttgtggtac tgatattcac
    15661 gcttttgccg gcagacagcc tttttttagc tacccacgtg tattaggtca tgaaatatgc
    15721 gccgaagcgg tttcgcgagg cagccagtgc caaacagcac aatcaggcca gcgctattcc
    15781 gtcatcccat gcattccgtg tggcgagtgc gcagcctgtc gggaagagaa aacgaactgc
    15841 tgcgaacgtg tttcgctgta tggcgtgcat caggatgggg gttttagtga gtaccttgcg
    15901 gtacgtgaag acaaccttgt gcctctccct gacgaggtca gcgacagtgc cggagcattg
    15961 gttgaatgtt tcgccattgg tgcacatgcc gttcgtcggg cagagatcaa ggctgaacaa
    16021 aacgtactgg tgattggtgc tgggccaatc ggtttggcta ccgcagccat cgccagggct
    16081 aaaggggcgc atgttgttgt tgctgatatt gactgtcaac gtcgccagca cgttgtggat
    16141 catctggcaa ttaatgtctt cgacccaaca caggaaggtt ttattgccgc gcttagtgaa
    16201 gtatttggag gcgaactggc ttgcgtagta ctggatgcga cgggaaataa agcttcaatg
    16261 agtcatgatg taaatcttat tcgtcatggc ggcaaaattg ttttcatcgg tttgtacatt
    16321 ggtgaacttg ttattgacga tccgaccttc cataaaaaag agacaacgtt actcagcagc
    16381 cgcaatgcca cacgggaaga ttttgcgttg gtgattgaac tgatgcgcag caataaaatt
    16441 cacgaaaatt taatgaaaaa ccaggcgttc aatttcttta gtgttggcga agattaccag
    16501 cgtaacgttg tagaaaataa aaatatggtc aagggtgtga tcacttttta agggaacaaa
    16561 tatgacaact gtttatgtca gtgaagaaaa tttaaaatca ctggtgcacc acaaattaca
    16621 taccgccggg ctggataccg acaccacaca acaggtgaca gatgttttag tacatgccga
    16681 tattacaggt gtgcattcac atggtgttat gcgtgtcgaa cattattgca cccgccttgc
    16741 tgccggaggg ttaaataaag ccccgcagtt tagcattgag caaatttcac cgtcagtggc
    16801 cattctcgac tctgacgacg gaatggggca ttccgcatta attagcgcca ctgagcacgc
    16861 tattaaactg gcccagcaag agggcctcgg ttttgttagc gttaaaaaca cgtctcactg
    16921 tggtgcgtta tcttattttg cagagatgat caccaacaaa gggttggttg ctatcgtaat
    16981 gacgcaaacc gatacctgtg tggctcccca tggcggcgcc gagcgctttt tgggaactaa
    17041 ccccatcgcc tttggtttcc cggtggaaaa cagccatccg atgattgttg atatggcgac
    17101 cagtgccacg gctttcggca aaatacttca tgcaaaggaa accggaaaac atattggtga
    17161 agggctggcg atagataaaa acggttatgg caccactgat ccgtataaga ttgaaaacct
    17221 gctacctttc ggccaacaca aaggttcagg cattgcactg gctattgatg cactgactgg
    17281 cgtgctgatg aatgcgaatt ttggcaacca tattgttcgc atgtatggtg attatgacaa
    17341 gatgcgtaag ctggcaagtt tggttattgc catcgatccg aaaaagctcg gcaatcctgt
    17401 ttttgcaaaa accatggcga aaatggtcac ggagctgcat gccgttaaac cggctcccgg
    17461 tgtcgaaaaa gtgttagcgc cgaacgatcc gcaaatacac tacaaagaaa aatgccaaca
    17521 ggaaggtatt ccggttccgg caggaatatt ccattatctg gcagagaatt aatcactact
    17581 ttttattata acgatagaaa agcgctgtat ttatcgtttc tgttatatta accaggagca
    17641 ggatatgaaa gcacttcgtc ctctgacggc atcgcttatg ctgttaacca gttgtttatt
    17701 aatatctaat accacactgg caaaaacaac attaaaactg agtcataatc aggataaaag
    17761 ccatgctgtc cataaagcat taagctattt agcggataaa accaaagagt attctaatgg
    17821 tgaattagtt attcgcattt acccaaatgc aacattaggt aatgagcgtg aatcactgga
    17881 attaatgaac tccggtgcat tacagatggt taaggttaat gccgcatcac tagaatcatt
    17941 tgctccagat tacagccttt tcagtctgcc gttcttattc cgcgaccgtg atcactatta
    18001 tcgtgttctg caaagtgatt taggtaaaaa aatacttaat tcatcagaaa gcaaaggttt
    18061 tgttggtatt acgtactatg acggcggtgc gagaagtttt tattccaata aaccgattac
    18121 aaaacctgaa gatttagcgg gaatgaaaat cagggttcaa caaagcccca gcgccattgc
    18181 aatgatgaaa gcactcggtg gtgtcgctac cccgatggcg caaggcgaac tctacaccgc
    18241 acttcagcaa ggagtggttg atggcggaga gaacaatacc gttgtctatt ccgatatgcg
    18301 tcacgccgaa gtcgcaaaag tctattcacg tgatgaacac accatggtac ctgatgtgtt
    18361 aattatcagc accaacgtgt tgaataaact tggtgataaa gagcgcacgg cattattaaa
    18421 agcagccgat gagtccatga tgcagatgaa ggacgtcatc tggcctgcgg ctgaaaaaga
    18481 agcctacgac aaaatgaaag ggatgaacgc aacagttgtt gatgttgata aatccgcttt
    18541 caaagaacgc gtcaaaccgt tatacgatga attcaaggcg aaagatgcac aatcagccaa
    18601 aaaccttgag ctagtagaaa gtatgtaacc ctaatgggca ggtaattcct gccttttcat
    18661 ggaagactta tgcgaacact cactcatatt cttaataaga tccttgccgg atgttgctgc
    18721 attattctcg ccattatggt gttctgcgta acctagcaag ttattgcacg ttttattttt
    18781 aacgctccca gcactgtgct tgatgagttc acccagattt tatttatgtg gatgatttta
    18841 cttggcggtg tatatactgc ggggttaaaa aaacatctgg ccattgattt gttggctcaa
    18901 aaacttccgg ctgcatctgt tctgacgctt gacagtttta tccaaattat tattacggtc
    18961 tttgcggtta ttttcatgat ctatggcggg aatattgttg ttgaaaaagc acaacatgtt
    19021 ggacaaatct ctccagtatt aaaatggcca atggataaag tatattgggt tatgcctgtc
    19081 agtggactta ttctggtctg gtattcagta atgaatatta ttgacaacta tcgtaaacgg
    19141 aattttcatt aacgagggaa atatggactt tgaatatatc taccccgtct taattttatt
    19201 tggcagtttt gccgtcatgc tggcaatcgg tgtgccaatt acttttgcga ttggtctttc
    19261 ttcgctgtta tctattatta ctgccttacc acccgatgcc gccatttctg tgatttcgca
    19321 aaagatgact gtggggctgg atggctttac gctattagcc attcccttct tcgtgttagc
    19381 cggaaacatt atgaataccg gtggtatagc cagacgactg gttaacctgg cgcaagcatt
    19441 agttgggcgt cttcctggct cactggctca ttgtaatatc ctcgcgaata cgctgtttgg
    19501 tgcgatttca ggttcagccg ttgcgtcagc cgccgcggta ggtggaatta tgtcaccact
    19561 gcaagaaaaa gagggctatg atccggcgtt ttcagcagcg gttaatattg cctctgcccc
    19621 cattggcctg atgatcccac cgagcaatgt gttgattgtt tattccctcg ccagtggcgg
    19681 gacttctgtt gctgcgttgt tcttagccgg atacttgcca ggcattctca ccgctgctgc
    19741 tttaatgttt gtggcggcac tttatgcgcg acgtaaccat tatccggtgg ccgaacgtat
    19801 caattttcat caatttttgc aggtattccg cgaatcaatt cccagcctga tgcttatttt
    19861 tatcattatt ggcggtatta tcgcaggggt attcacgccc acggaagcat cggcaattgc
    19921 ggtaatttat agtttagccc tggcgatgat ttaccgggaa atcactttta agaagctcaa
    19981 tgatattctg ttagattcgg tagtaaccag ttcaattgtt ctgttactgg taggctgctc
    20041 gatggggatg tcatgggcca tgacgaatgc tgatgttcct gagttgatca acgaactgat
    20101 taccagtgtt tcggataaca aatgggttat tctgtttatc atcaatatca ttctgttgat
    20161 cgtcggtacc tttatggata tcacaccggc gatcctgata tttacgccta ttttcctgcc
    20221 gatcgatcag catctgggaa tagatcccat ccacttcggt attattatgg tgttcaactt
    20281 gaccattggc ctttgtacac cacccgttgg caccattctg tttgttggtt gcagcattgg
    20341 taaggtcagt atcgacaggg caataaaacc attactgccg atgtttctgg cattgtttgt
    20401 agtaatggca attatttgtt atttcccgca gcttagtctg atgctgccag gattattttc
    20461 gacctgatac acaatgaaga ctgcatactg agtaattttc aatgccccac cactctgtgg
    20521 ggcattgaat tggtccatta cggtaccgga ttgaccaaca gttgcccaat cgaaccacgg
    20581 tccgccattt ccagcgtctg actgttgaag tagaacggga agtgcgccca ggaaggctga
    20641 ccgaaataaa ccagcagctc cacttgtccg tcaacccaaa cggtatcttt ccagcctctg
    20701 tcttccggga acggcatcgc accattcacg ttacggatct ggaacatcac gccttcaata
    20761 tggaacgctt gcggctcgtc cgcgcgtacc gtccagcgtt cccacgttcc ttgctgcgca
    20821 gtaacatcaa tacggttgac gtcccacaac tgcccattaa tccccgggtc atcacccaga
    20881 ctgatatcac gacttcgaat cggcgaaccg gccataattt cagttggcag caagcgcatc
    20941 ggaagactgt ctgtgaccag cggcagaagg ccggttgggc gcagcgttag caccagggta
    21001 gaaaccagaa tactggatgg ctcaaagaag ccgcgaatac gatcaacaat gctcgccgct
    21061 tcaccacagg tgatcgacac ttcatcaccg ttgctcatat ccaccagaat ctcacgacgc
    21121 tcgcccggtg ccagcgaaag ttgcttcacc gatacaggag cgggcaggaa tccctgatcg
    21181 ccagaaatca catgtaacgg gcgaccatcg ctcatctgta gttgatagcg acgagagttc
    21241 gacgcgttca acagacgcaa gcgcacccag ccacgcgaga cttcaacgta cgggctttgt
    21301 acaccgttaa ccagcagcgt atcgccaaca aagccgccgc ttcccggttc gttgtattct
    21361 ggcgtaccaa agttatccag ccgtttatcc tgaataatga ccggaaaatc atccacacca
    21421 taatggttgg ggataggcag cgacttgctg acttcatctt ccaccagcca cattccggca
    21481 aggccgttat agacctgctg agccgtgcgg ttgggagtat tggcgtgata ccacagagta
    21541 gctgcgttct ggcgaatagg cagtacgggt gcccagtcag cgtttggcga catcatccgt
    21601 gccggaccgc ccatcagcgg gccaggtacc tgtagcccgg ccaccgtcat tgagacattt
    21661 tctgtcaggc ggttgctgta aataagctta acatcgtcgc ccttccagac gcggatagtc
    21721 ggccccaggt aacgaccatt gattccccaa accgacgcgc gtgtccctgg cgtaaatgac
    21781 cagtgcgcac gttgtacagt cataaacagc ggttgcccac ggcgagattc aagcagcggc
    21841 ggaacgggta gcggttgctg ttgcccggct gcgctggcct tcaggggaac agcgcctgca
    21901 caaagtgcaa tccccgatgc ctgaatgaac tgacgccgac tgagtgacat atttgctcca
    21961 tgtaaaactg gctaaagtaa taccgtgcag gaaagtgttc cccgcaaaaa tacagattgc
    22021 cgacttaaac ttttccggcg gcttcgcgct ctgcgacttc tttatcgagc tcagcgattt
    22081 tttgttccat tatcgaacga caatgggcag ccaactcgcg aacctgatct ttgccatact
    22141 gactgacgtc aattggcggc agcatttcga caatcaccag accgttgtgt agccgattaa
    22201 gattaatctt attcgaagtt gtagagacgc acacgggaat aatcgggacg cccgccgcaa
    22261 ttgccgcgtg aaatgccccg gtcttgaacg gtagcaggcc gcgaccacgg ctgcgggttc
    22321 cttccgggaa catccagatg gaaatacggc gttttttgaa gtgattcacg acttccgcaa
    22381 tagtgccgtg agctttagtg cgattgtttc tgtcgatcaa taagttaccg gttaaccagt
    22441 acaactgccc gaagaagggg atccacagca agctcttttt acctaccgtt accgtcggcg
    22501 gttgcacaat gttcgatgct gtcaccatgt catagttgtt ctggtggtta gcgatataga
    22561 tagcattgcc gtaactttca gcgtctgcag gtttgcggca ctccactttc aggccgaaca
    22621 gcggcgcaag acggccaaac atatgcccaa aggtggccac atgtttcgga ttacgcgggc
    22681 tgaaaaggca gtaaatggag ccgaatacac agactaaaat gctgtaaatc acggtaataa
    22741 taagacgaaa gatatatagc ataacaccct ctgaaggtct gagagactgg cattgtacgt
    22801 cacctgaaat caggttaggg cattgttaaa agcagctctc tggaaaagcg tattgttaat
    22861 cgcaacgcat gaataaacaa cggtttcaag ggaattttta tcgtttccgg gggttgaata
    22921 cgctgccgga tgacgactta acgtttcatc cggcgttcct tgcaagcggg aggaaacagc
    22981 gccctccccg gcatattact cttcgctatc accgctgctg gcacggcgag gagagtcgat
    23041 ctcaacacga tcgatacgct gcaaaccgcg catcaacgta ccgcggcgtc cacgttcgcc
    23101 agtgactttc tgtagctctt ccggacgcag tttaattttg cgtttcccaa catgaatggt
    23161 cagcgtgctt tgcggtggta gaacgtacag ttgcgccaga ccatcctcgc cacgcgcggc
    23221 ttctgccgac ggaatgttga taatcttatt gcctttgccc ttcgacaact gcggcagatc
    23281 gcttaccggg aacatcaaca tacggcctgc ctgagtgatt gccagcagca tatcggaagc
    23341 atcctcaatc accaccggcg gcataacatg ggcattttcc ggtaaggtga tcaaagcctt
    23401 acctgcacgg ttacgcgcca ccagatcgtt aaaggtgcag acgaaaccgt aacccgcatc
    23461 ggaagccatc agcagtttct gatcgtcgct ttccatcagc atatggtcaa cggtcgctcc
    23521 aggcggcaac gttaatttgc cggtgagcgg ttcgccctga ccacgcgccg acggcagcgt
    23581 aatcgggtcg atggcatagc tacgaccggt ggaatcaaca aacactaccg gttggttgct
    23641 cttacctttc accgccgctt tgaagctatc gcccgcttta taattcaggc ccggcgcgtc
    23701 gatatcatgg cctttagcgc tgcgtaccca gcccatctgc gacagcacaa tggtgacagg
    23761 ttcagacggc agcatgtcgt gctcgctcat cgctttcgct tcttcgcgtt cctgcaacgg
    23821 cgaacgacgt tcgtcaccgt aggcttgcgc gtctgcctgc agttctttct tcagcaggtt
    23881 attcatttta cgctcggaag ccaaaatgcc ctgcaactgg tcgcgctctt tttccagctc
    23941 actctgctca ccgcgaatct tcatctcttc cagtttggca agatgacgca gtttcagttc
    24001 gaggatcgct tccgcctggg tttccgtaag gccaaaccgc gacatcagcg ccggtttcgg
    24061 ttcatcttca ttacgaatga tctcaatcac ttcgtcgata ttgagaaacg ccaccagcaa
    24121 accttcgagg atatgcaggc gcttgaggac tttctccaga cgatagttca gtcggcggcg
    24181 cacggtatcg cggcggaaca ccagccattc ggagaggatt tccagcaggt ttttcaccgc
    24241 cggacgacca tccagaccga tcatattgag gttaatgcga tagctctttt ccagatcggt
    24301 ggtagcgaag aggtggttca tcacctgatc catatccacg cggttggaac gcggcacaat
    24361 caccagacgg gtcgggttct cgtggtcaga ttcatcgcgc agatcgtcaa ccatcggcag
    24421 ctttttgttg cgcatttgcg cagcaatttg ctccagtacg cgcgcacctg aaacctgatg
    24481 cggcaatgcg ctaataacca ccgcgccatc ttctttcttc caaaccgcgc gcatacgcac
    24541 tgaaccacgt ccgttctcgt agattttacg aatctcggcg cgcgaagtga taatttccgc
    24601 ttcagtcgga taatccggcc cctgcacgat atccagcagc tgatcgagcg tggttttcgg
    24661 ctggtcgatt aatgcgattg ccgcctgagc cacttcacgc aggttatgcg gtggaatatc
    24721 ggtcgccatg ccgacggcaa taccggtggt gccgttaagc aaaatgttcg gcaggcgggc
    24781 tggcagcatt ttcggctcct gtaaggtgcc gtcgaagttt ggcacccagt cagccgtccc
    24841 ctgtccaagc tcgcttaata gcaactcaga atatttcgat aaccgggatt cggtgtaacg
    24901 cattgccgcg aacgatttcg gatcgtccgg cgcgccccag ttcccctgac catcaaccag
    24961 cggataacgg taagagaacg gctgcgccat caggaccatc gctccataac aggcaatatc
    25021 gccgtgcgga tggtatttac ccagtacgtc accgacggta cgggccgatt ttttaaattt
    25081 ggcactggca ttcagaccca gttcagacat cgcatacaca atgcgccgct gaacgggttt
    25141 cagaccatca ccaataaacg gcaacgcacg gtccatgatc acgtacatgg agtagtttaa
    25201 gtaggcgttt tccgtaaatt catgtagcgc aaggcgctct gccatatcgc tcattaattc
    25261 tgattcctca acttattcgc cagccttcaa aggggcaata ttgccggaga tactacatta
    25321 tctgccgcgt cgagtcacag gagatcagcg tgaatagcgc cgccggacgt gctccgacgg
    25381 cgataaagac atcaatgtgc cttgatatac aactctttgc tgtagacata atcctgggga
    25441 ttatgcagcg gaaaaccgcc aacgtagggt ttaagcagtt tgattaacgg ctgataatag
    25501 acaggaatga gcggtgcctg ctggttgatg atcacttctg cctgctgata caacgcatta
    25561 cgctttgttg catcagccgt ttgtgccgcc tggtttagta aggcgtcata ctgcgcgttt
    25621 ttccagtggc cgacattttc ctcgctatcg ctcttaagcg tgttcagaaa ggtggaggca
    25681 tcattgtacg tcgcatccca cgactgccga gacaacatga aatcaccggc tcgtcgggca
    25741 tcaagataag ttttccactc cattgtgcgc agcgtcacct gtgcacccag ccattttttc
    25801 cattcggaag acaacgctat cgcggtcttt tcatgcagat cgtacttgtt gtagaacagc
    25861 tcaaagcgaa gcggatgaga ggcgtcgtat cccgcctgtt tcaacaaggc ttttgccatc
    25921 gcgacgcgct cactcagcgg cttttacagt tcatcgaacg tcgtcgcgct aaagcctttt
    25981 acctctggcg gcgtcagcgt agttgcgggc gttctcaacc ccagtacctt ttgcgcaata
    26041 agctgtcgat caaccgtaag atatagcgcc cgacgcaccc gcacatcgtt aaatggcggt
    26101 ttctcaaggt tgaagttgta atattcgctg ttcagacgcg gaataattcg tagctcgcca
    26161 ggcagtgatt tttcaatggc gggaatttgc tgcgccggaa cccaggtgag atcgacctct
    26221 cccgcgcgat agcggttata gccggtgacc gaattatcca gcgccagata ctcaacctgt
    26281 tgcaatactg tatgttgcgc atcgcggtac tttggatttt tgcgtgcagt aatcttttcg
    26341 ttaactaccc actgatcaag cacaaaggca ccgttgtaaa ccatgttctc tggcttactc
    26401 cagctatcgc catgtttagc gatgacatga tgaggaaccg ggaacagcgt cggccaggcg
    26461 agcatcgtcg tgaaccaagg aactggctgc tcaagcgtaa cttcaagagt acgatcatcc
    26521 gtcgctttga cacccagcga tgtaacatct gctttacccg caacaatagc cgcggcattg
    26581 ttaatgtgcg cctgtgccag atagccagca aaagggcttg ccgttttcgg gtcaaccgcg
    26641 cgctgccagc cgaggacaaa atcctctgcc gtcagaggct gaccgtctga ccactgcaaa
    26701 ccgctacgca gatggaaaat atagcgcttg ccgccgtcca gtatctccca gcgttcagcc
    26761 tgagcgggct gcacctggcc ttcaccgtcc atccatacca gaccttcaaa cagatccagc
    26821 acaatctgcg cggcagtatt ctcctccacc ttttgcgggt cgagcgtgcc tgggtcgcta
    26881 tgattgttgt aacgaaagac ttgttgcggg gcgagcggtg tgttggcggg aacgtcagca
    26941 gcgtagagag gagccgcact taccaggctg accagccata ataaatttcg cgtatacatg
    27001 gtattcctta ccggagaaat tataagccca acaacaatac gcctgtggca ttgttcgctc
    27061 aaacttatcg cgagtgattt ggctcacact ccggtaaaga ttcttcatca cgccaacggc
    27121 acataaacat ctgtttccag tcgctgctct gtcatccctt cggcaagatt ggtgtagtga
    27181 aacaggatcg gggccttgcg cattttttcg ccgcttgccg gaagccagtg gcgaatgatg
    27241 ccccatatcg tgtgtgaaat atcgtccagc tcgccaacat ggcgggccac agcataacgt
    27301 ccaccggtaa gttcaccatt acttacgcca taacgattat cggggatcgg ttcgctaacg
    27361 ctgccgcaga tatcaaagcg aaacgcttct ggtgcggtgg tggcagggtc gtcccaggcg
    27421 acgccaaaag tctggctttg gttaacagga gaaagccccg tctctttacg ccacatgata
    27481 aatttcgcca cactgtagtt gagcaactca gggctacagc ggtgcggcaa catcgccaca
    27541 gggattgatg ggaaatcgat aatatttaca tccagtgtca ggtttgtcat tttgcctccc
    27601 tgcgaaatta ttcccgggaa cgtgtcccgg gagcggtaac aattacggat ttactttgcg
    27661 gatctgtttg acgtcaattt caacagaatt ccagtcttta tcgacttcac cctgaatctc
    27721 aaccgtatct ttcggcgtca ccgtcatgcc gttccagcgt ttgtggtcga tatcaacatt
    27781 gatagtaccg ctggcatctt tgaacacgta gagatcgtca gagatgcgtt caacgatatt
    27841 gccgcgcagg gttacccagg tgtcgtcacg cagggatttt gcgctttcta tagtcgttac
    27901 gctgccattc ggcccctgga atcctccggc ctgactttgc gttgccgatg ggccagaaaa
    27961 acctccctgc tctgctgcca taaccggtgc actgcacagg gccattactg cgattactgc
    28021 tgcgtatttt ttcatgttta ttactccctt taatgtctgt ttccgagcat ttaacaagat
    28081 agtccttaac aacttcttaa gggaaaaaaa taaaatttaa tgctgtacag agcgcgttac
    28141 aacacggttt actggcagca aatacggtta tcgcagggat gaaaaaatgc gaattttact
    28201 gatagaagat gacatgctga ttggcgacgg catcaaaacg ggccttagta aaatgggttt
    28261 tagcgtcgac tggtttacac aaggtcgtca gggaaaagag gcgctatata gcgcacctta
    28321 tgatgcggtg atcctggatt taaccttacc gggcatggat ggtcgcgata ttttgcgcga
    28381 atggcgagaa aaaggtcagc gtgagccggt tttgatcctg accgcgcgcg atgcgctggc
    28441 ggaacgtgta gaagggctgc gtctgggagc tgacgattat ctgtgtaaac cttttgcgtt
    28501 gatagaagtc gccgccaggc tggaagctct gatgcgccga accaacggcc aggccagcaa
    28561 cgagctgcgc cacggcaacg tcatgctcga ccccggcaaa cgtatcgcca cgctggctgg
    28621 cgaaccctta acgctgaagc caaaagaatt tgccctgctg gaattactga tgcgtaacgc
    28681 tggtcgggta ctgccgcgca aactgattga agagaaactg tatacctggg acgaagaggt
    28741 caccagtaat gccgttgaag tgcatgtgca tcatctgcga cgcaaactcg gcagtgattt
    28801 tattcgtacc gttcatggta ttggctacac attaggtgag aaatgaaatt tacccaacgt
    28861 cttagtctgc gagtcaggct aacgctaatc tttttaattc tggcctcggt gacctggctg
    28921 ctttccagct ttgtcgcctg gaaacaaaca acggataacg tcgatgagtt gttcgacacc
    28981 caactgatgc tgtttgccaa acggttaagt acgctcgatc tcaacgaaat caacgcggcg
    29041 gatcgcatgg cacagacgcc aaataaatta aaacacggtc atgttgatga cgatgcgctg
    29101 acctttgcca tctttaccca cgacggcaga atggtcctta acgatggcga taatggagaa
    29161 gatattcctt atagctatca acgagaaggt tttgctgacg ggcaactggt gggcgataaa
    29221 gatcaatggc gttttgtctg gatgacctca cctgatggca aatatcgcat tgttgttggt
    29281 caggaatggg aataccgtga agacatggcg ctggcgattg ttgccggaca attgatcccg
    29341 tggctggtcg cactgccgat tatgttaatc atcatgatgg tactactggg tcgtgaactc
    29401 gcgccgctga acaaactggc gctggcacta cgtatgcgtg acccagactc ggaaaaacca
    29461 ctaaacgcga cgggcgtacc cagcgaagtg cgcccactgg ttgagtcgtt gaatcaactg
    29521 ttcgcccgca cacatgcgat gatggttcgt gaacgacgct ttacctccga cgcagctcac
    29581 gaacttcgta gcccgttaac ggcgctgaaa gtgcaaaccg aagttgctca gctctctgac
    29641 gatgatccgc aggcgcggaa aaaagcgctg ctccaattac attccgggat cgatcgcgct
    29701 actcgtctgg ttgatcaact gctcacgcta tcgcggctgg actcactgga taaccttcag
    29761 gacgtcgcgg agatcccgct tgaagatctc ctgcaatcgt cggtgatgga tatttaccac
    29821 acggcgcagc aggcgaaaat tgacgtgcga ctgacactca atgcccacgg catcaaacgc
    29881 accggacaac cgctgttgct aagtttgttg gtacgaaacc ttctggataa cgccgtgcgc
    29941 tacagtccac agggctgcgt ggtggacgtc acgctgaatg ccgataattt catcgtgaag
    30001 gataacggcc ccggtgtgac gccagaggca ctggcgagaa ttggcgaacg cttctatcgc
    30061 ccacccggac aaaccgctac cggcagtgga cttgggcttt caattgtcca gcgaatcgcc
    30121 aagctgcatg acatgaatgt tgagtttggg aatgcggaac aaggtggatt tgaggcgaaa
    30181 gtaagctggt aagggtaaga cttttgctaa attcgcaaaa gactttgcac attttgctaa
    30241 tttcaccgta ccgctctgtg acgtactata gtcggcaaac gtctcacctt aaggttaaaa
    30301 aatgagcaac atcctgatta tcaacggcgc gaaaaaattc gcccactcca atggtcaact
    30361 gaacgacacc ctgaccgaag tcgcggatgg cacactgcgc gacctcgggc atgatgtccg
    30421 catcgttcgc gccgacagcg actacgatgt caaagcggaa gtacaaaact ttctctgggc
    30481 tgatgtggtg atctggcaga tgccaggctg gtggatgggc gcgccgtgga cagtgaaaaa
    30541 atacattgat gatgtattca ccgaaggtca cgggacgctg tatgccagcg atggtcgtac
    30601 ccgcaaagat ccgtcgaaaa aatacggctc cggcggcctg gtacagggca aaaaatatat
    30661 gctttctctg acctggaacg caccaatgga agccttcacc gaaaaagatc agttcttcca
    30721 cggcgttggt gttgacggtg tgtacctgcc gttccataaa gcaaaccaat tccttggcat
    30781 ggaaccgctg ccgacattta tcgctaatga cgtgataaaa atgcctgatg ttccccgcta
    30841 tactgaagaa tatcgcaagc atcttgtgga aatttttggt taactagagc tctggcttta
    30901 gaaggagtta accatgatta cagtaatcgc agaaatccgt actcgtcctg gtcaacatca
    30961 ccgtcaggca gtgttggatc agtttgctaa aatcgttcca accgtactga aagaagaagg
    31021 ttgccacggc tatgcgccaa tggtggattg cgctgctggc gtgagtttcc agtctatggc
    31081 accggattct atcgtgatga ttgagcagtg ggaaagcatc gcgcatcttg aagcgcactt
    31141 gcagaccccg cacatgaaag cgtacagcga ggccgtaaaa ggcgatgtgc tggagatgaa
    31201 tatccgcatc ctgcaaccgg ggatttaaca ttcctgaaaa gaggccggtg ttgctaccgg
    31261 tctcagccca gattagccat tctttcttcc cgttttcagt cctcgatgga aatcgttaat
    31321 tcgcttaatg gttttaaccg cacgctgcgg cactgcggaa gctaccgacc agacaatcat
    31381 ttccagacac aacaataccg taccatgtag cggcgttttc cctttttcat ccccgcgcgg
    31441 aacatgaatc acgatactgg cgtctttgct aaaacgtgaa tccagcgcgt ttgtcagcaa
    31501 aataacaggg atccccagcc gcctcgcctc acgaagcgtt gtcagccctt cccggtgcgc
    31561 agatttttgc gccatcatga tcagaacatc gccacgttga agcgcgatca gttgctcggc
    31621 aaggccaata cccgtacggt ttagtgccgt ggcaggcaaa cctatgcggt tgaatagtcg
    31681 tgcggtatag tccgccagga tacccgaagc gccaatgcca aaaatagcga cttgccgtgc
    31741 ctgcaccaac aacgctacag cttgcgcaag cgcgtggcgg ttttcaggct ctgacaggac
    31801 attacaggta tacaaatgcc cttcaagaac aaagtcgatt gcggtgttaa catcgctcgt
    31861 caagttactc accgtcgtag acatcttttc gctggaacta agggctggac caagccactg
    31921 ttccagagtg cgtttcagat cacgcagccc ggcaaacccc agggcctgta tagccctgat
    31981 aactgtggca tcggaagtgt tcaacgtggc cgcaatttcc atcgccgttt gctcgagtac
    32041 cacttcacga tgctcattaa tgtagctggc gacaactctt aacccaggcg ttaactgact
    32101 cgcgcgagtg cgaaaacgct caccaaaaat atcaacccgc gctttatctt tctggatcat
    32161 ttatcctccg gtagaggtcg ccctgcaatt tgtgattgta tctgcgcagc catcagcccc
    32221 agagatgcaa aggagttaga aatcgcctct tcacgagaaa tcagcacata acgcccggaa
    32281 cgacaggcag tcagaaactg acaccatccc ggcatgactt tttccattgc tgccagctca
    32341 tcctgaggtt taccaccggt gtcaccgcgc caggttgcaa aaacaaaatc ggcatccagt
    32401 tcaggtaaac gctccgcgct cacatccatt cgcccacctt caggaatgct ctcaatcagc
    32461 ggcggaaaac gaaaaccagc atcgcgtaat acccgcccca gcgaatgata gctatgcatc
    32521 acattaattt ttccctggtt tgcctgaatc accgataccg ttattttttg gctgtccagc
    32581 gtcgccttca gcgcattgat ttgtgcctga tagcggcgtt caagtatcgc cagctgcgac
    32641 tgcgttcctg taagttctgc cagcttgcga tagatttctg gcgcgccccc tttgagatga
    32701 tcaatgctca ccgtgggcgc aattttttca agttgttcaa tgggcgtatt acgggtcggt
    32761 tctgtgatga tcaggtcagg ctttgccgcc acaatcgctt caatatcaat atcagcggta
    32821 ccaataaagg cgatcgatga attatcaaag tcgacacctg tcaataacgc gccagatcga
    32881 atgaaatggc taccgtcagg ccgagttcgc ccgtggctgg cgacaggagg tacaccgagc
    32941 tcaatcagag gaatagtgat atcaagatcg tgtaaagaga caattctctt tggatgagca
    33001 ggaacagtga ctttgcgccc taaatcatca gtaaatacct gaacaggttc tgttgcacgg
    33061 gaaaaaaatg acaacagtat cagcagagaa aaaaataaac gcatataact cccgttaaaa
    33121 actgttcctg cgctgccaga gcagtagcaa aaaaaatggt ccaccaatca gtgagataat
    33181 gattccggca ggcagttgca aaggcaaaaa agccaaacga ccaatgctat ccgccagaat
    33241 aactaacaac gcccccgata ccgcgctccc cagcagtaat gtcgtctgcc cgccacgtag
    33301 aataaaacgt gatatatggg gtgctatcaa tccgacaaaa ccgatgttac caacacacga
    33361 aacgcagacg gctgtcagga ttatgggtgc agcgacacgt aacaacgcca gacgagaact
    33421 attcacccct aacccggttg caacctgatg accaagcaga gcgatatccg ccgtcctggc
    33481 ggtgaacagc aataaaagag cggctggcaa catccaacag gctgaaatag cgactaacat
    33541 ccagttagct gcatgcaggc ttcccgataa ccacatcaat gcagtttgta catctcgaac
    33601 atctgcagtg gtcataaata caccgatacc ggcggcgaaa aaccaggaaa caccaatacc
    33661 aatcagaaca aatcgcgggc gggaaatatc gcgcgcacaa aaaatcacga ttaaagccac
    33721 caacaggcca ccagcaagac cagccactgg acgccagaac attcccagca ttggaaactg
    33781 gaaaatcagc cacagcacag ccacgctaca tccttctttg acgccgatta aaccaggatc
    33841 ggcaaggccg ttacgtgcaa tggattgcat tgcggcccct gccataccta acatggcccc
    33901 gcatagcaat gccatgatga ctctcggcaa gcgaatatcc tgcacaatat aacgtgcgtc
    33961 tgtgcttacg ttttctggag caaacaacgc tcgtcctact tccgatgccg gaacagagaa
    34021 agaaccctgc atcagaccga atatcagtat tccaaccgca agcaggaaca gcccaccaag
    34081 caacagcaga tttttagggc gtaccagtgt cgaaaactta ccaacacgta gtgggcgtag
    34141 cccgacgttc attatccgac tcatttgaac atcctcgtcg ccataatgac aaaaacaggc
    34201 gcaccaacta acgccgtcat gacaccggtc gctaactcat ggggtgtgaa aagcgtacgg
    34261 gcaataatat ctgccagtaa taacaaaagt gcgccgacac aagctgaaag agggagcaga
    34321 acgcgcagat ccgctgatac cagacgccgg acaatctgcg gtactagcag gccgacaaaa
    34381 ccaatggggc cagcaatcga tacggctgcg ccacagagca acgcgatcgc aagcagggtg
    34441 aatgttcgcg tgcggaatac agagacgccc agcccctgcg ccatgcgatc gcccagtgcc
    34501 aacatgttaa gggagggtgc aaggtaaagg gctaacacaa atccccccag agaaaaccag
    34561 gcggacgtac ccagcgtcgc ccaatcctgc cccgctaaat cacctgctag ccatgtccgc
    34621 atcgccagca gtgtttgttc gtcaagaatc aggattgcag ccgttattga ggacacgaac
    34681 gccgatagcg ccacgccgca taaagtaact ttcattggcg ttaaccctga acgccctgcg
    34741 gaggaaagca gcaatatcaa caggaacaat aacgcgccgc ccgtagatgc cagcagtggg
    34801 cgtccaaccg gaaaggccag cccaagcgca ctggcagcaa cgacagcaag agcggctccg
    34861 gcatttaagc ctaaaatatg tggctccccc aatgggttac gaatgacagc ctgcaacaaa
    34921 gcaccagcaa ctccaagaga agcaccggta agcaatgctg ccgctaaacg cggcaaacgg
    34981 agtcttacaa ttacgttgtg atcgaagttt tgcggatcaa aatgaagaaa tgcctgcaac
    35041 accgtttgtg gtgcaataaa tcgggcacca atccctggat ataccacgca tcccaccagc
    35101 agtaaggcga ggaaaattac agtaaccagc cctgtgcgca tcatactttt tctacaccac
    35161 gaaacggcat gaagaacggc tttcccgtca gaggatttat tgatgcgtga acatcaacgt
    35221 caaagaccgc ttttaccagc tcaggcgtgc aatgctcccc ctcattcaac acacgcacca
    35281 ctttcccctg gcgtaaaaat atcaacgtat cgccataatt gacggcaaaa ttcagatcat
    35341 gcaacaccac gacgactgta cgcccgtgat gacgcgttaa atcatgtaat aactcaagaa
    35401 tttcaacctg ataacgtaaa tcaagccagg ttgtgggttc atccagcagg atataaggcg
    35461 tcttttgcgc caacaccatc gcaatccagc aacgctggcg ctgcccacca gagagttttt
    35521 caacaggtaa atgtgcaaac tcctgcgtac ccgttaattt cagggcctct tctaccgcca
    35581 gctcatcggc gtcgctccat tgacgaatga aattctgcca gggaaaacgc ccacgcgaca
    35641 ccagttcata aacggttaag ccctccggca ataatggcga ctgcggcaag atcccaagtc
    35701 tacgggataa cgctttcgtt ggttgctcgt gaatcgcttt accatccagc aatatcgaac
    35761 cgcccatggg ttgcaagata cgagcaatgg tgctgagcag cgtcgacttt ccacagccgt
    35821 ttgccccaac cagaacggtc attttttctg tgggaacgga gaaagaaaca ccatcaacaa
    35881 tgattttctt gtgatacccg gcagaaacat tgtcgaggat cagcccctgc ccttttgcgt
    35941 tatgtgccac gtgtacgcca acttaatcaa agacaaacaa aaataaaact cattctcctt
    36001 tgagagtttg acgccacaaa atgtagtagt caagcagtta aaagccacac aaaaacaagg
    36061 taaataacag gtaaaatatt tttgcacata aaaacaaaac cattaaatat caataaatta
    36121 aaagcaatca aattgtataa acttttcata aaaacacatg tagcaatttt ttgttatttc
    36181 tcatttacta ctacattact gcgtgattga atgattctca attacatatc cgatggcgaa
    36241 ataacaaacg gatagtgctt agtgagcaat aagaaacaat tttcactcgc gacaatccgc
    36301 gagtttttgg gataacagac taaatggcta tgttcacacc ttcattctca ggactcaaag
    36361 gtcgggcgct cttttcactg ctttttgcgg caccgatgat tcatgcaaca gactctgtaa
    36421 cgaccaaaga tggcgaaaca atcactgtta cagcagatgc aaataccgca actgaggcaa
    36481 ccgatggtta tcaacctctg agcacttcca cggcgacatt aaccgatatg ccgatgctgg
    36541 atatcccgca ggtggtcaat acggttagcg atcaggttct ggaaaatcag aatgcgacga
    36601 cgctggatga agcgctttat aacgtcagta acgtggtaca gaccaataca ttaggcggaa
    36661 ctcaggacgc ctttgtacgt cgtgggtttg gtgctaaccg ggatggctcc atcatgacca
    36721 acggcctgcg aactgtactt cctcgcagtt tcaacgccgc cacagaacgt gtggaagtgc
    36781 taaaaggtcc ggcctccacg ctgtatggca ttctcgatcc tggtggactg attaacgtcg
    36841 tgaccaagcg cccggaaaaa acattccatg gttcggtttc agccacctcc tccagttttg
    36901 gtggcggcac tgggcaactt gatatcacag gtcccattga aggcactcag ctggcgtatc
    36961 gccttaccgg ggaagtgcag gatgaagatt actggcgaaa cttcggtaaa gagcgcagta
    37021 catttattgc cccgtcactc acctggtttg gtgataatgc aacagtaact atgctctatt
    37081 cccatcggga ctataaaact ccgttcgatc gtggaacgat tttcgacctt acgacgaaac
    37141 agcccgtaaa cgttgatcga aaaatacgtt ttgacgaacc gtttaatatt acagatggaa
    37201 agtccgatct ggcgcaactc aacgcagaat atcatctcaa tagccagtgg acagcgcgct
    37261 ttgattacag ctacagccag gataaataca gcgataatca ggcgcgtgtt accgcgtatg
    37321 atgcaacgac aggaacactg acacggcgtg ttgatgcaac tcagggatct acccagcgta
    37381 tgcatgctac tcgtgcggat ctgcaaggga atgttgatat tgccggattc tataatgaga
    37441 ttctgggtgg ggtgtcatat gaatattatg atcttctgcg tacagatatg attcgctgta
    37501 aaaaagctaa agatttcaat atctacaacc ctgtttatgg taataccagc aaatgtacaa
    37561 cggtttcggc gtcggacagt gatcagacga tcaaacagga gagctactca gcgtatgcac
    37621 aggacgcact ctatctgacc gataactgga ttgccgtcgc cgggatccgc tatcagtatt
    37681 acacgcagta tgcgggtaaa ggccgtcctt ttaatgtcaa tactgacagc cgcgatgaac
    37741 aatggactcc caaactgggg ttagtctaca aactgacgcc atcggtatcc ttatttgcca
    37801 attattcgca aacatttatg ccgcagtcgt caattgccag ctacattggc gatcttccac
    37861 cggaatcatc taatgcttac gaagtcgggg caaaattcga gctattcgat ggtatcaccg
    37921 cagatattgc gctgtttgat atccataaac gtaatgtgtt gtataccgaa agtgttggtg
    37981 atgaaaccat tgccaaaacg gcaggccgtg ttcgttcaag aggggtagaa gtcgaccttg
    38041 cgggagcatt aactgaaaac attaatatca ttgccagcta cggctatacc gatgctaagg
    38101 ttctggagga tcctgattat gcagggaaac cattgccgaa tgttcctcgt cataccggtt
    38161 cgctattcct gacttatgac attcataaca tgcctggcaa taacacactg acgtttggcg
    38221 gtggtggaca tggcgtaagc cgtcgttcgg caaccaatgg ggctgactat tatctgccag
    38281 gctatttcgt tgccgatgcc ttcgccgcat acaaaatgaa attgcagtat ccggtcacac
    38341 tgcaattaaa cgtcaaaaac ctgtttgata aaacgtatta cacctcttcc atcgccacaa
    38401 ataatctggg caaccagatt ggcgatcctc gtgaagtgca attcacggtg aaaatggaat
    38461 tttgatcaaa aaagcccggt atacacttgt ataccgggct atgttttatt cttctggcgg
    38521 gaggattatc agacctcaat ctctgccatg tcgcctttct cttgcaacca gttgcggcga
    38581 tcttccgagc gtttcttcgc cagcagcata tccatcatcg cgtcagtacg ctgatcgtct
    38641 tcatcatcga tagtcaactg caccagacgg cgagtgttcg gatcaagcgt ggtttcgcgc
    38701 aattgcatcg ggttcatttc cccgagacct ttaaaacgct ggacgtttgg cttgcctttc
    38761 ttgcgtttta attgctcaag tacgccctct ttctcttctt ccgtcagcgc gtaataaacc
    38821 tctttcccga ggtcaatacg gtagagcggt ggcagtgcaa cgtaaacgtg accgtgtttc
    38881 accaatgcgc ggaagtgttt tacaaacaaa gcgcagagca gcgtggcaat gtgcagacca
    38941 tcggagtcag catccgccag gatacagatc ttgccgtaac gaagctggct caaatcgtcg
    39001 ctgtcaggat cgataccgat agctaccgaa atatcgtgca cttcctgcga agccagcact
    39061 tcgtcggaag agacttccca ggtgttaagg atcttacctt tcagtggcat gatcgcctga
    39121 tattcgcgat cgcgcgcctg cttggcagat ccgcctgcgg agtcaccttc cacaaggaac
    39181 agctcggtac ggttaaggtc ctgcgcggta cagtcagcca gtttgccagg caacgccggg
    39241 ccgctggtca gctttttacg caccactttt ttggccgcac gcatacggcg ctgggcgctg
    39301 gaaatcgcca tctccgccag cagttcagcc gcctgaacgt tctggttcag ccacaaggtg
    39361 aaggcatctt tcaccacgcc agaaacaaat gccgcgcact gacgcgaaga gagacgctct
    39421 ttggtctgcc cggcaaactg tggatcctgc atttttactg acagcacata ggcgcagcga
    39481 tcccagatat cttccgccga cagcttcacg ccgcgcggca gaatgttgcg atattcacag
    39541 aactcgcgca tcgcatccag caggccctga cgcaagccat taacgtgcgt accaccctgc
    39601 atcgttggga tcaggttgac gtagctttcg gtcagcagtt caccgccttc cggcagccac
    39661 agtagcgccc agtccaccgc ttcagtatcg ccagcgaaat taccgataaa cggtttttct
    39721 ggcagcgtcg gtaaaccgtt taccgcttcc gccaggtaat cattcagacc gtcctgatag
    39781 caccagcgct gttcggtgtt gttgatctca tctttaaagg tgatctcaac accagggcac
    39841 agtaccgctt tggctttcag cacatgcgtc aggcgtgaaa cagaaaaacg cgggctgtca
    39901 aagaaggttt catccggcca gaagtgcacg ctggtaccgg tattgcgttt accgcaagtg
    39961 ccgataacct gtaaatcttg caccttttcg ccattttcaa aggcgatgtt atagatctga
    40021 ccatcgcggc gtacgttaac ttctacgcgc ttcgacaggg cgttaaccac cgaaatcccc
    40081 acgccatgca ggccgccaga gaactggtaa tttttgttag agaatttacc gcccgcgtgc
    40141 agacggcaaa gaatcagttc aaccgccggt accccctctt ccgggtgaat atctaccggc
    40201 atcccgcgcc catcgtcaat cacttctaac gactggtcgg catgtaagat tacatccacg
    40261 cgttttgcgt ggcccgccag tgcttcatcg acactgttat caataacttc ttgccccaaa
    40321 tggttagggc gagtggtatc ggtatacatc cccggacggc ggcgaaccgg ctcaagcccg
    40381 gtgagtacct caatggcatc agcgttataa gtttgcgtca tggtttaagt tagcaattcg
    40441 agttgatcgt cagagatggt gcagaccaag aaaatcgacg atcgggttga aataatcttc
    40501 gaagcccgtg aatgcgtggt tgccgccttc tatgacagtc tggcggcagg aagcgtagta
    40561 cgccaccgcc tggcggtaat ccagcacttc atctcccgtc tgttgcagca gccagatcaa
    40621 atccggcgct tccagcgggt caatctgcat gactttgaga tcgtaaatat ggcgtgactc
    40681 tagcacatat tgctgcccgg tgtaggggtt ctcgttctga ccgagatagt ccgtcagcag
    40741 ttcaaacggg cgcaccgccg ggtttaccac cactgcgggc agcataaaac attgcgacaa
    40801 ccaggtggcg taatatcccc ccagtgacga accgacaata cccagcgaat cgccgccatg
    40861 ttccaggaca atggattcca gcagttctgc cgcatcggaa ggatacggcg gcaactgcgg
    40921 aatgatcatc tctacgtcag ggtgatgttc cgccaaccag ttttttaaca agctcgcttt
    40981 tgcagagcgc ggcgagctgt tgaaaccgtg taaataaaga agcgtagaca tcagtagcct
    41041 tctgaagcgg tatcaggttg gaagcgtgcg tccgccaggc gatgcacctc ggtggtcagc
    41101 gtgccatcag catgtaactc gagagtacgc cagccgggcg cgatggtgtc cagcgtaaag
    41161 ttagaacagt gcggcttaaa ctgcacacag gtcgacggcg ttgccagcaa gcggcgaccg
    41221 ttccagtcga gatccagctc ctgatgaata tgaccgcaca gcaagtattt gacgtgcgga
    41281 aacttcgcca gcacggtatc cagttcgccc gcgttacgca ggctgtgttg atcaagccaa
    41341 ctacaacccg caggtagcgg atgatgatgc agcagcagca acgtatggcg ttctggcgca
    41401 tcggccagtt tacgttccag ccactcaagc tgaaactcgc tcagctcacc gtgcggcacg
    41461 ccaaacacct ggctatccag caacaggatt tgccattgct caccaataaa cacccgcttc
    41521 gccggggaga tacccgcatc ctgtaacgcg ctgtacatcg cgggctggaa atcgtggttg
    41581 cccggcagcc agacgcaggg cgcacgaaaa cttgcgatgc cttcagcgaa atgctgatag
    41641 gccgcagagg attgatcctg cgctaaatca cctgtcgcga caatcaggtc gaattcgtgc
    41701 tggtgtggcc gaatcgcctc cagcaccgcc tggtagcttt cccaggtgtt tacccctaac
    41761 agggcttcgt gcttttgtgc aaacaggtga gtgtcggtaa tttgtaaaat cctgactctg
    41821 gcctcaccag ccagaggaag ggttaacagg ctttccaaat ggtgtcctta ggtttcacga
    41881 cgctaataaa ccggaatcgc catcgctcca tgtgctaaac agtatcgcaa ccagtccgct
    41941 aaaaactgat taatttgatg cttttcgtcg cgttgatgca actttttatt aggataatca
    42001 taccgcgctt tgaagcgaaa aatctgctgg cttgaacaca cttcagccac catcgcgtca
    42061 tgatacagac gcaccgtcat tgacggaagg ctccagtaac tgatcgcggg cgcagtctgt
    42121 tctattgtca ccagggtagt gtatcgggtc gattccacaa tcgtcagccg atattgtgcg
    42181 tttgccacct gatagcttac agtttcgccg ggtgcgtcat tgcgcggtaa caaacggcgc
    42241 aattgtgaaa agttcatctc gcacaggcgc atcatttcag gaaagtcagg tgtgtaacgc
    42301 ttcatttatg cccactcatt ttttaacgct tgatgatgca gctgcagcca ttgcaaagcg
    42361 atgaccgacg ctgcgttgtc gattttcccc tcttctaccc actggtatgc ctgttcccgg
    42421 cttaccacat gaacgcgaat atcttcgttt tcatcagcca gaccgtgaat accgcttgcg
    42481 gtcgtggcgt ccacttcgcc caccataatt gacgaacgct cactggtgcc ccccgggctt
    42541 gccaggaaac ttaacaccgg tttggtccgt ttgactatca gccccgcctc ttcaatcgct
    42601 tcgcgacggg caacatcttc cacactttca ccctcttcaa tcatcccggc aaccatctcc
    42661 agtagccagg gggtttcgct agtgtcgtac gcggcaattc gaatctgctc aatcagcaca
    42721 acttcatcac gtactgggtc aaagggtagc aagactgcgg cgtgaccgcg ctcaaaaatt
    42781 tcccgccgta cctcatgact catttgcccg ttgaatagac gatgacgaaa tctataaaga
    42841 tctaatgaaa aaaagccgcg ataaagtgtt tctcgtgcaa taatttctac atcgtttttg
    42901 ccaaatgtaa cgggcaggtt gtctggctta agcattgtta atgtcctggc actaatagtg
    42961 aattaaatgt gaatttcagc gacgtttgac tgccgtttga gcagtcatgt gttaaattga
    43021 ggcacattaa cgccctatgg cacgtaacgc caaccttttg cggtagcggc ttctgctaga
    43081 atccgcaata attttacagt ttgatcgcgc taaatactgc ttcaccacaa ggaatgcaaa
    43141 tgaagaaatt gctccccatt cttatcggcc tgagcctttc tggtttcagt tcgttgagcc
    43201 aggccgagaa cctgatgcaa gtttatcagc aagcacgcct tagtaacccg gaattgcgta
    43261 agtctgccgc cgatcgtgat gctgcctttg aaaaaattaa tgaagcgcgc agtccattac
    43321 tgccacagct aggtttaggt gcagattaca cctatagcaa cggctaccgc gacgcgaacg
    43381 gcatcaactc taacgcgacc agtgcgtccc tgcagttaac tcaatccatt tttgatatgt
    43441 cgaaatggcg tgcgttaacg ctgcaggaaa aagcagctgg gattcaggac gtcacgtatc
    43501 agaccgatca acaaactttg atcctcaaca ccgcgaccgc ttatttcaac gtgttgaatg
    43561 ctattgacgt tctttcctat acacaggcgc aaaaagaagc gatctaccgt caattagatc
    43621 aaaccaccca acgttttaac gtgggcctgg tagcgatcac tgacgtgcag aacgcccgcg
    43681 cacagtacga taccgtgctg gcgaacgaag tgaccgcacg taataacctt gataacgcgg
    43741 tagagcagct gcgccagatc accggtaact actatccgga actggcggcg ctgaatgtcg
    43801 aaaactttaa aaccgacaaa ccacagccgg ttaacacgct gctgaaagaa gccgaaaaac
    43861 gcaacctgtc gttgttacag gcgcgcttga gccaggacct ggcgcgcgag caaattcgcc
    43921 aggcgcagga tggtcactta ccgactctgg atttaacggc ttctaccggg atttctgaca
    43981 cctcttatag cggttcgaaa acccgtggtg ccgctggtac ccagtatgac gatagcaata
    44041 tgggccagaa caaagttggc ctgagcttct cgctgccgat ttatcagggc ggaatggtta
    44101 actcgcaggt gaaacaggcc cagtacaact ttgttggtgc cagcgagcaa ctggaaagcg
    44161 cgcatcgtag cgtcgtacaa accgtacgtt cctccttcaa caacattaat gcttctatca
    44221 gtagcattaa cgcctacaaa caagccgtag tttccgctca aagctcatta gacgcgatgg
    44281 aagcgggtta ctcggtcggt acgcgtacca ttgttgatgt gttggatgcg accaccacgc
    44341 tgtacaacgc caagcaagag ctggcaaatg cgcgttataa ctacctgatt aaccagttga
    44401 atattaaatc agctctgggt acgttgaacg agcaggatct tctggcactg aacaatgcgc
    44461 tgagcaaacc ggtttccact aatccggaaa acgttgcccc gcaaacgccg gaacagaatg
    44521 ctattgctga tggttatgcg cctgatagcc cggcacccgt cgttcagcaa acatccgcac
    44581 gcactaccac cagtaacggt cataaccctt tccgtaactg atgacgacga cggggcttcg
    44641 gccccgtctg aacgtaaggc aacgtaaaga tacgggttat ctgccgcatt cttccccctt
    44701 ctcgcttcaa tttcgaccag ccatcctcta ttctgatggg tatttaccac tggtcccgga
    44761 agacaaaaat gaaacggaca aaatccatac gccacgcatc gttccgcaaa aactggagcg
    44821 cacgccatct gacaccagtc gctctcgcgg ttgccactgt ttttatgctg gctggctgtg
    44881 aaaagagtga tgaaacagtg tctctctatc aaaatgctga cgactgttca gctgcaaacc
    44941 caggcaaaag cgccgaatgt accaccgcgt acaacaatgc gctgaaagaa gccgaacgta
    45001 ctgcgccgaa atacgccacc cgtgaagact gtgttgctga atttggtgaa ggtcagtgcc
    45061 agcaggcacc agcccaggct ggtatggcac cagaaaacca ggcgcaggcc cagcaatcca
    45121 gcggaagttt ctggatgccg ctgatggccg gttacatgat ggggcgtctg atgggcggcg
    45181 gcgcgggatt tgcacagcag ccgctgttct cctcgaaaaa cccggccagc ccggcttacg
    45241 gtaaatatac cgacgcgacg ggtaaaaact atggcgcagc ccagccaggc cgcaccatga
    45301 ccgtaccgaa gacggcaatg gcaccaaaac cggcgaccac cactaccgtt acccgtggcg
    45361 gttttggtga atctgttgcc aaacaaagca ctatgcagcg tagcgcaacc ggcacctctt
    45421 ctcgttcaat gggtggctga taccgatgga aagagtcagt attaccgagc gcccggactg
    45481 gcgcgaaaaa gcccacgaat acggtttcaa ttttcacacc atgtacggcg agccgtactg
    45541 gtgtgaagat gcttactaca agttgaccct cgctcaggtt gaaaagctgg aagaagtcac
    45601 cgccgaactg caccagatgt gcctgaaagt ggtggaaaaa gtgatcgcca gcgatgagct
    45661 gatgaccaaa ttccgcatac caaaacacac ctggagtttt gtgcgccagt catggctgac
    45721 gcaccagcca tcgctttatt cgcgtcttga tctggcgtgg gatggcactg gcgaacctaa
    45781 gcttctggaa aataacgccg atacgccaac gtcactatac gaggcggcgt tctttcagtg
    45841 gatctggctg gaagatcagc ttaacgccgg taacttgccg gagggcagcg accagtttaa
    45901 cagtttgcaa gaaaagctga tcgatcgctt cgttgagctg cgtgaacagt atggcttcca
    45961 gttgctgcat ctcacctgct gtcgcgacac ggtggaagat cgcggaacca ttcagtattt
    46021 gcaggactgc gcgacggaag ctgaaatcgc taccgagttc ctctacatcg acgatatcgg
    46081 gttaggtgaa aaaggtcagt tcacggattt acaggatcag gtgatttcca acctgttcaa
    46141 gctgtatccg tgggaattta tgttgcgtga gatgttctcc accaagctgg aggatgcagg
    46201 cgtacgctgg ctggaaccgg cgtggaagag cattatctcc aacaaagcgc ttctaccgct
    46261 actgtgggag atgttcccga atcacccgaa cctgctgccc gcttattttg cggaagatga
    46321 tcatccgcaa atggaaaaat atgtggttaa accgatcttc tcccgtgaag gcgcaaacgt
    46381 gtcgatcatt gagaacggca aaaccattga agcagcggaa ggtccgtatg gcgaagaagg
    46441 gatgattgtt cagcaattcc acccgttacc gaaattcggc gacagctata tgctgattgg
    46501 tagctggctg gtgaacgatc aacctgccgg aattggcatt cgtgaagacc gtgcattgat
    46561 cacccaggat atgtctcggt tttatccgca tatttttgtt gagtaataca gcttaatatt
    46621 gccccgataa gcggggcaat ctcttataat ccaccattca taattcccat caatatataa
    46681 acacatacat atccaacctt tatttaacat taatactcaa tttaatgtta aatcgtcata
    46741 tgcaagcgat attgtaatta aagtaagtga atacatcaca acataactat ttgatattat
    46801 ttaaatactt tacttttatc tcaccagatt taaatttaat aattaaaaaa caatgttgca
    46861 gggatcacac tacacagtat tgaacattgt gattgatata ttgcccccct actatccatt
    46921 aaacataaat atttgtttca ccgaataaca acatatttaa aagaaacaca aaagcattct
    46981 cactctttat tgaattaata ttcatattca cgtaacgaat ttattagcac cctctcaaca
    47041 gtcgatattc atcattatta tatatttgcg ttatataagg agatatatcc atgtttgata
    47101 aatatagaaa aacactcgta gccggaactg tggcgataac cctgggtttg tcagcatcgg
    47161 gggtgatggc tgcgggtttt aaaccagcgc cgcctgccgg gcaactgggt gcggtcattg
    47221 tcgatcccta cggcaatgca ccactgaccg ctttggttga cttagatagc catgttattt
    47281 ctgacgtcag agttaccgtc catgggaagg gcgaaaaagg cgtagaaatc agctatcccg
    47341 tgggtcagga atcactaaaa acttacgatg gtgtaccgat ttttggtctt tatcagaaat
    47401 ttgctaacaa agtgaccgtt gagtggaaag aaaacggcaa ggtcatgaaa gatgattatg
    47461 tggtgcacac ttcggccatc gtcaataatt acatggataa ccgctctatc tccgatttac
    47521 aacagaccaa agttattaaa gtcgcaccgg gttttgaaga tcgcctctat ctggttaata
    47581 cccacacctt taccgcccaa ggttccgatc tccactggca tggtgagaaa gataaaaatg
    47641 ccggtatcct tgatgcgggt ccggcaactg gcgcactccc ttttgatatc gcgccattca
    47701 cctttatcgt cgatacggaa ggcgaatacc gctggtggtt ggatcaaaac accttctacg
    47761 atggtcgtga ccgcaacatt aacaaacgtg gttatctgat gggtatccgc gaaacgccac
    47821 gcggcacctt taccgctgta caaggtcagc actggtacga gttcgacatg atggggcagg
    47881 tgctcgaaga tcacaaacta ccgcgcggat ttgctgacgc tactcatgaa tccattgaga
    47941 cgccaaatgg cacggtactg ttgcgcgtag gtaagagtaa ctatcgtcgc gatgacggcg
    48001 tacacgtcac caccattcgt gaccatatcc tcgaagtcga taaatctggt cgcgttgtag
    48061 atgtatggga tctgacgaag atcctcgatc cgaaacgcga tgcactgctc ggcgcgctgg
    48121 atgcaggtgc agtttgcgtt aacgttgacc ttgcccatgc aggacaacag gcaaaactgg
    48181 aaccagatac accgttcggc gacgctctgg gtgtagggcc aggccgtaac tgggcgcacg
    48241 ttaattccat cgcttatgac gcaaaagatg actcaattat tctctcttct cgtcaccagg
    48301 gtgttgtgaa gattggtcgt gataagcaag tgaaatggat ccttgcaccc tctaaaggtt
    48361 gggaaaaacc gctggccagc aagctgctga aaccggttga tgctaacggt aagccaatta
    48421 cctgtaacga aaatggcctg tgcgaaaact cagacttcga ctttacctac acccagcata
    48481 ccgcctggat ttccagcaaa ggaacgctca ccatttttga taatggcgat ggtcgtcatc
    48541 tggaacaacc tgccttacca accatgaaat attcccgctt tgtggaatat aagattgatg
    48601 agaagaaagg caccgttcag caagtgtggg aatacggtaa agaacgtggc tacgatttct
    48661 atagcccaat cacctccatc attgaatatc aagccgaccg taacaccatg tttggcttcg
    48721 gtggttctat tcatttgttc gatgtcgggc agccaaccgt cggtaagttg aacgaaatcg
    48781 attacaaaac caaagaagtg aaagtggaaa tcgacgtgct gtcagataaa cccaatcaga
    48841 ctcactatcg tgcattgtta gtccgtccac aacagatgtt caaataatta agaagggaat
    48901 aaaaatgtcg aaattaggga tctcatcact atttaaaagc atactgctta ccgcagcttt
    48961 ggctgtttca tttaccgctt ctgcatttac cgaaggcacg gattacatgg ttctggaaaa
    49021 acctattccg aatgcagaca aaacgctgat taaggtattc agctacgcct gcccgttctg
    49081 ttacaagtac gacaaagcgg ttaccggtcc ggtatcggaa aaagtgaaag atatcgttgc
    49141 tttcacgccg ttccatctgg aaaccaaagg tgaatacggc aaacaagcaa gcgaagtctt
    49201 tgcggtcttg atcaataaag ataaagcagc aggcatttcc ttatttgatg ctaactctca
    49261 gtttaagaaa gccaagtttg cctactacgt ggcttatcac gacaaaaaag aacgctggtc
    49321 tgacggtaaa gatccggctg cttttatcaa aaccggtctg gatgccgcag gtatgagtca
    49381 ggcggatttt gaagcggcac tgaaagaacc agcggttcaa gaaacactgg aaaaatggaa
    49441 agcctcttat gatgtcgcca aaatccaggg ggttccagcc tatgtcgtta atggtaagta
    49501 cttgatctac accaagagca tcaaatccat cgacgcgatg gcagacctta tccgtgagtt
    49561 agcaagcaaa taacagggaa acaggctatg ggtattaagg gaatgtggaa agatcttcgc
    49621 acatcaccag ttgacacact ggtcagatgg caggagcaac gcttgttgtg gttgttaatg
    49681 gccgttgcga tgggcgcgtt aattatcctg gcgcactctt tcttccagat ctatctctac
    49741 atggcaccct gtgagcaatg tgtatacatt cgctacgcca tgtttgtgat ggttattgga
    49801 ggattggttg cagcgatcaa cccaaagaac atcatcctaa agctaattgg ctgcgtgatg
    49861 gcattttacg gcagcatttt gggactgaag ttttcgctca aactgaacga tatccaccat
    49921 gcagtacata atccggatcc cgattcactg tttggcgtac agggttgctc taccgatcca
    49981 acttttccct ttaacctgcc gctggcacag tgggcaccaa attggttcaa gcctaccggg
    50041 gattgtggct atgacgcacc aatcgttcct gatggcgtca cactgagcag cacgcaacag
    50101 tggtttgtgg aaatgtatca gcaatctgaa ggctggtatc tgctaccacc atggcatttt
    50161 atgaatatgg ctcaggcttg tatgctggct tttggtatgt gtctggtgct gctggtcatt
    50221 atgagtggcg catgggcact taaaatcatc agaggctaac tcagcaacac ccgaagccag
    50281 tcattctggc ttcgggttct tttattaacc tatctgcacc gacagcatac ttaaactgcc
    50341 catttctata tcatcgaccg gaatggtaat tggctcctgc ccatcccacg cacctaagac
    50401 atacaacaac ggcagatagt gctctggcgt tgggttcgat aacgcgccac cttcatggtc
    50461 gagataattc accagaggat gttgttccac cggcccttgc cacgtcagat tcgctttcac
    50521 atactcatta aacgacgtcg cccacggata cggtgaacta tcaccgtgcc acttcactgt
    50581 acgcaggtta tgcaccacgt tgccactggc gaccaacatt atcccttcat cacgcagcgc
    50641 cgcgagtttg cgccccattt cgaaatgcca ggcggcaggt ttgctactgt cgatactcaa
    50701 ctgcaccatc gggatatcgg cgtcaggata catcttaatc agtacgcccc acgagccgtg
    50761 gtcgaagccc caggcttctt tatccagcgt cacagggacc ggcgctaaca gctcaaccag
    50821 atgctgtgcc agcgcaggtg aacccggagc aggataatgc gtatcgtaca gcgcctgcgg
    50881 gaagccacca aagtcatgaa tcgtgggcgg cgtctccatc gcggtcactc ctgttccacg
    50941 ggtaaaccag tgagccgaaa ccaccactat tgcttgcggg cgtggcaatg tcatccccaa
    51001 cgtctgccag ctgcgggtat acaaattatc ttccagcacg ttcatcggac taccgtgacc
    51061 taaaaacaat gctggcatac gtgttgaaga catgatgata tccttaacta aaggtgtcat
    51121 tttgatatcc tcacaatacg cttgttcggc ggagtaagaa cccggataac aatgatgatg
    51181 atcatcagtt attttgacga tctgcctgaa ggtgaagatt tataaggagt tgtcgatgtc
    51241 agtacctctc attctgacca tactggcggg ggcagccacg tttattggcg cgtttctcgg
    51301 cgttctcggg caaaaaccct cgaaccgctt actggcgttt tcgctaggat ttgcggcggg
    51361 gatcatgttg ctcatctcat taatggaaat gcttcctgcc gcattagccg ccgaaggaat
    51421 gtcacctgtg ttgggttatg gaatgtttat cttcggtctg cttggctatt ttggcctgga
    51481 ccgcatgttg ccacatgcgc atccgcagga tttaatgcaa aaatcggtgc agccgttgcc
    51541 aaaatcgatc aagcgcacag ccattctgct cactctcggc atcagtctgc ataacttccc
    51601 ggaagggatt gccacctttg tcacggcgag cagcaacctg gagctgggct ttggcatcgc
    51661 actggccgtt gcgttacaca atattcctga aggtctggcg gttgcaggcc cggtttacgc
    51721 ggcaacgggg tctaaacgca ccgcgattct ttgggcgggg atttctggac tggcagaaat
    51781 tcttggtggt gtgctggcgt ggttaatcct cggtagcatg atttccccgg tggtcatggc
    51841 ggcaatcatg gcggcggttg caggaattat ggtagcgctc tcggttgatg aattaatgcc
    51901 gctcgccaaa gagattgacc ctaataataa ccccagctat ggcgtactat gtggaatgtc
    51961 agtgatggga ttcagtttag tgctgctaca aacggcggga attggttaaa agcaacatca
    52021 gaagttgtcc agcgcatgga caacttctgc cagcttcact aaagccttat tctacctttt
    52081 cggctgtctc ttctctcgta ctgtttaagt atttgttccg cgcttcaagc agtgcgtctc
    52141 tgtcctggcg aaacgcccga caataacgtt tcaaatgcag gaaataaccc acaacaacaa
    52201 gagcaatcaa tacaatccag taccaggcga taaacatcct tttaccctta atatttaaca
    52261 atactaaaca tttagcgcat aaatttcaca tatccttttt cggatatatt catatggtcg
    52321 cataataacg gaacaattat atgattttga tcataatgaa aaagaatgcc tgatattaat
    52381 gaaattttct catagaaaga gaagcggaat taacatcttg catagcaggg aaaaataaaa
    52441 accgaagcct gctacaactt cggtttcttg attaaggcag taaattaagc agcggttttc
    52501 agctggcttt acgctcatgt gcctgacggt atgccaccag gtcttcaata gtcacgagcg
    52561 ccatattgtg tttattggca aactcaatgc actctggtgc acgcgccatc gtgccatcgt
    52621 cattagtcag ctcacacagc acaccagccg gtttaaagcc tgccagcgtc attagatcaa
    52681 tagttgcttc agtatggccg ccacgcgtca gtactccacc tgcctgcgcg cgaagtggga
    52741 aaacgtggcc cgggcgattc agatcagaag gttttgcgtc atcggcaatc gctgcgcgaa
    52801 cggtcgtaat acggtcagcg gcagaaacac cggtagtcac accttcagcc gcttcaatgg
    52861 tcacggtaaa accggtgcca taggcgctgg tgttattttc taccatcatt ggcagatcga
    52921 gttgtttacg gcgatcttca gtaatgcaca ggcaaacaat accgctaccg tggcgaatgg
    52981 tcagcgccat ctgctcaacg gtcatggttt ctgccgggaa gatcatatcg ccttcgtttt
    53041 cacggtcttc atcatcaagc accattacac cgcgtccttc acgcagcgca gccagtgcat
    53101 tttcaacacg ttcgaaaggc gtaccaaaag aggaaagtag cgtctgattc atggtaaaaa
    53161 aacctcacta aaattatggt taccagaatc agggcagtct taggagtggc ggcatatagc
    53221 caaaataacg tgagcgggtc catgcccgac agaatcgtta ctctctccca tccggactct
    53281 aaccgtcggc cccggaatta caccggatct gctgtccttt gagttcgcac ccaaagcgct
    53341 cgcgggcttt caactgagtt gatttaccgc cggtggggaa tttcgccccg ccctgagaat
    53401 aagcggattc actataacgc taatgattag cggcagcaac gcatagcttc acataattct
    53461 ggtttatgac ttactcttat cgcactacaa tggcactcaa cacctatcag tacagggaaa
    53521 ctacaatgat tgacccgaaa aaaattgagc aaatcgctcg ccaggttcac gaatcaatgc
    53581 ctaaaggaat cagggagttc ggggaagatg tggagaaaaa aatccgccaa accctacaag
    53641 cgcagctgac acgtctcgat ctggtaagcc gcgaagaatt cgacgttcag acgcaagtcc
    53701 tgttacggac tcgtgaaaaa ctggcgttac tggagcagcg catcagtgaa ctggaagcgc
    53761 gcaataattc ggtagcagat cttcaatctc ctccggctat cccgcctatt gataaagcag
    53821 aataaagcaa cacaaaaacg ggccagacgg cccgtaatat cagtgttcta attcaagttc
    53881 tctggtttgc atcatttgtt ggcaatacca ggcataacgt gcgcaaaacc aggttctatg
    53941 ttcttcatcc atgttaccag taacggccag aatatcgacc gggcgacctt ctgcgtgcat
    54001 tctggcaaaa ctacgcgcca ggaaatcgaa attatttaaa gaattaagac tatgatccat
    54061 aagcacatcc tccggttatc gcataccttt taaggccata tgctttttct gatttcagta
    54121 ttccccaatc agtgtagtgc ctttaaacat aacgtgcgta aatatattgt ccccgatcac
    54181 actttttagt gaaaaataaa aaaccatatt aaaagacttt ctcttccaga acttctccaa
    54241 catgtgttat tattagcaaa gacaaatgta agcaaggaaa ctatcccaaa gtaaatattg
    54301 cgtcactaaa tggacattgg agtgacatat gattttattc gccgactata acacccctta
    54361 tcttttcgct atttcctttg tactgttgat cggcctttta gaaatttttg cgcttatctg
    54421 cggtcacatg ctctccggtg cacttgatgc acatcttgac cattatgatt ccataaccac
    54481 aggtcatatt agccaggcac ttcattatct caatatcgga agattgccag ctctcgtcgt
    54541 tctttgctta ctggctggtt tttttggtct tattggcatc ctgttgcagc atacctgcat
    54601 catggtctgg caatcaccac tctcgaacct gtttgtggtt cctgtcagtt tactgtttac
    54661 gattattgcg gtgcattata ccggtaaaat agttgcgccc tggattccac gcgatcatag
    54721 ctcagctatt acagaagaag aatatattgg tagcatggcg ttaattaccg gtcatcaagc
    54781 cacttcaggt aatccctgcg aaggaaaact caccgatcaa tttggccaaa tacattattt
    54841 actactggag cctgaagaag gaaaaatttt cactaaagga gacaaagtgt taattatttg
    54901 ccgactttct gcaactcgat atttggcgga aaacaatccc tggccccaaa tattgtaatt
    54961 tattaatcaa aaggaaattt aataatggat gatattgtta attctgtgcc ctcctggatg
    55021 tttaccgcga ttattgccgt atgcattctg tttattattg gaattatttt cgccaggctc
    55081 tatcgtcgcg cttcggcaga gcaagctttt gttcgtactg gtttaggtgg gcaaaaagtg
    55141 gtaatgagcg gtggcgcaat cgtgatgccg atcttccatg aaataatccc catcaatatg
    55201 aatactctga agctggaagt cagccgctca accattgata gcctgattac gaaagatcgt
    55261 atgcgcgtcg atgtcgttgt cgctttcttt gtgcgggtaa aaccttcagt agaagggatc
    55321 gccaccgctg cccagacgct ggggcaacgc accctgtcgc ctgaagactt acgtatgttg
    55381 gttgaagata aatttgtcga tgccctccgt gcaacagctg cgcaaatgac catgcatgag
    55441 ttacaggata cccgcgagaa cttcgtgcag ggcgtacaaa ataccgttgc tgaagatctg
    55501 tcgaaaaacg gcctggaact ggaaagcgtt tcacttacca actttaacca gacgtcgaaa
    55561 gaacatttca atcctaacaa cgcctttgac gccgaaggtt taaccaagct gacgcaggag
    55621 acggagcgcc gtcgtcgcga acgtaacgaa gttgaacagg atgtagaagt tgcggtgcgt
    55681 gagaaaaacc gtgatgcact ttcgcgcaag ttggagattg aacaacaaga agcgtttatg
    55741 acgcttgagc aggagcagca ggttaaaacc cgtaccgctg agcagaatgc gaaaattgcg
    55801 gcttttgaag ctgaacgtcg tcgtgaagca gagcagacgc gaattctggc tgaacgacag
    55861 attcaggaaa cagaaatcga ccgcgaacag gccgtccgct caagaaaagt tgaagctgaa
    55921 cgtgaagttc gcattaaaga gatcgaacaa cagcaggtca ccgaaatcgc caaccagacg
    55981 aaatcgatcg ctattgccgc caaatcggaa cagcagtcac aagcggaagc gcgtgccaac
    56041 cttgcacttg cagaagcggt aagcgcccag caaaacgtag aaaccactcg ccagaccgca
    56101 gaagccgatc gtgctaaaca agttgcccta atcgctgccg cgcaggatgc agaaaccaaa
    56161 gcggttgaac tgaccgtgcg ggcgaaagca gagaaagaag ccgcagagat gcaggcggcg
    56221 gcgatcgttg agttagccga agcaacacgc aaaaaaggcc tggcggaagc agaagcgcaa
    56281 cgtgcgctga acgacgctat caacgtactt tctgacgagc aaaccagcct taaattcaaa
    56341 ctggcgctat tacagtcgtt acctgcagta atagagaaat ccgttgagcc gatgaagtcc
    56401 attgacggca ttaagattat tcaggtcgat ggattaaacc gaggtggtgc tgcgggggat
    56461 gcggcatcag gcagcgttag tggcggaaac ctggcagaac aggcattgtc tgccgccctt
    56521 tcttaccgca cacaggcacc gctgattgac tccttgctca atgaaattgg cgtttcaggc
    56581 ggctcactgg ctgcattgac ttcaccctta acctcaacaa ctcccgtcgc cgaaaacgta
    56641 gaataaattt tcagctatga acatccctgg cacaaaaagc cggggatttt ttatatctgc
    56701 gttccgctaa aaactgcaaa tgatcaggtc gttgcaacgt tttgcgtgac cgctcgggga
    56761 aggcaaaact gcctcaggga aagcgttgcg cggggtccgg cgctcatcaa caatcgggga
    56821 gtcagcaaag ggctgaaacg ggaaagcccc tcccgaggaa ggggccttac agaaggaaag
    56881 ggttatgatg aagctcgtca tcatactgat tgtgttgtta cttgtaagtt tcgccactta
    56941 ttaacagcca atcagagggg ggagaaatcc tccctcaccc ttgttccttt actttaggtt
    57001 gaaaaaacaa cagcgtcaag aaggtttcat taacacatat ccctggccca aaaagccggg
    57061 gattttttat atctgcgttc tgctaaaaag tacaaatgat caggttgtta cagcgttttg
    57121 cgagccgact cggggaagag aaaactgcat aagagaaagc attgcgcggg gtctggcgct
    57181 catcaacaat cggggagtca gcaaggggct gaaacgggaa agcccctccc gaggaagggg
    57241 ccttatagaa ggaaagggtt atgatgaagt tcgtcatcat actggttgtg ttgttactgt
    57301 taagtttccc gacttactaa caactcatca gaggggggag aaatcctccc ttacccttgt
    57361 tcctttactt taggttgaaa aaacaacagc gtcaataggt ctatccggca caacgcgaga
    57421 actgtgaagc gctttccggt tagccttttt tatcctgttg gatcttcttg ataatgttag
    57481 tggtcgagca accgtcttca aagttgagca ccagcacttc gccaccattg gcccagactt
    57541 ctttactccc ggcaatctct tctggtttat aatcgccgcc tttcaccagc aggtctggca
    57601 ggatcccggc gatcaagcgc tgcggcgtgt cttcttcaaa cgacaccacc cagtcgaccg
    57661 cttccagtgc gcccagcaca atcatacgct gttcaagcgg gttaacgggg cgggaatccc
    57721 ctttcagccg tttggtggag gcatcgctgt tgacggcgac aatcaaacgg tcacccagtt
    57781 tgcgggcatt tgccagataa gagacgtgtc cggcgtgcag gatgtcaaag acgccattgg
    57841 tcatcaccac tttttcaccg cgtttacgcg ctgccgctac agcaagcttc agttcctctt
    57901 cggtcatcac gccaaagccg gtatctgcac gtccacgtac tgcgttttcc agctcgatcg
    57961 gcgaaaccgt ggatgtcccc agtttgccga ccaccacacc agccgccgca ttggcaaaga
    58021 agcaggcttc ttccagcgaa ttacccgctg ccagcgttgc cgccaggacg ccaatcaccg
    58081 tgtcgcccgc accggtaacg tcatacactt cctgcgcctg ggttggcata tgcagcggcg
    58141 ctttacccgg ttgcagcagc gacataccct gttcggaacg ggtcactaac agagccgaga
    58201 gttcgtaatc ggcaattagt ttcatgccgc gctcaacaat ctcttcttcg gtcttacatt
    58261 taccgacaac agcttcaaat tccgagagat tcggcgttaa cagcgtagcg ccgcggtagc
    58321 gctcaaaatc ggtacctttt ggatcaatca gcaccggaac acccgcttta cgcgccagtt
    58381 ggatcatctg ctgtacgctt gccagcgcac ctttggcgta gtcagaaagc accagcgcgc
    58441 caatcgaact cagcgcctga ttaatccgtt cgtgcagcgg ttgcggatca acaccttcga
    58501 aaccttcttc aaaatccaga cgaatcagct gttggttgcg ggaaagcacc cgtaatttgg
    58561 tgatagtcgg atgcgtcggt acagaaacga agtcgcattt gacgttgacg tcggccagag
    58621 atttactcag cgcgcgcgct gcatcgtcaa tgcccgtcaa cccgaccagg cgtgcattag
    58681 caccgagaga agcgatattc atcgccacgt tagccgcgcc gcccggacgt tcttcgatgg
    58741 tattcacttt aaccacgggc accggcgctt ccggcgagat acggctggtg ggaccgtacc
    58801 agtaacgatc cagcatcaca tcaccaacca ccatcactcc tgcacgttca aactctggca
    58861 gcgttacttt cattcctgtc tcctgagaga ttcaaaattt gcgcgcgata ataccatact
    58921 tcattcttcc accagccact tctgccagct tacccgcacc tgttcacgct ctgcggtgaa
    58981 gcaatcctcc ggcacatggc ccggcaattc ctgtaatgcc agatgatgaa gttcatcgcg
    59041 cagcgtagtg taagcacggg tcagcgccat cgcttcctgc tcttccataa tgtcgttttg
    59101 cgccagcagt tccagaatac gtacgttgtc tgaccagcgc gttaacttcg gtttttcatg
    59161 agcgtagcgc aacaccagat attgggtaat aaattcgata tcggtaattc cgccttcatc
    59221 ggctttgata tcaaagcgat cgcgatgttt attaccgaga tgagcgcgca ttttctcgcg
    59281 catttcccgc acttccgttt gcagagtttt accttctcgc ggcagcgtca taatctcgcg
    59341 acgcaccgcg tcaaagtgcg cggtgagctg cggatcgccg tacactacac gcgcacgcac
    59401 cagcgcctga tgttcccacg tccaggcctc gtttttctga taatcagcaa atgcttctgc
    59461 ggatgtcacc agcattcccg ccgccccgga tggacgcagt cgagcatcca cttcatacaa
    59521 aatgccggaa gaggttcgcg tactgaacag atgcataatg cgttgcgcca gacgcagata
    59581 aaactgtcgc ccgtcgattt cccgctcgcc gtcagtcatc gcatccattg ggcaatcatg
    59641 gaggaagatc aggtcaagat cggaactgta gcctaactcc cagccgccca gcttgccgta
    59701 gccgaccacc gcaaaaccac gcccttcgcg ctcgttcagg tgattcggtt taccgtagcg
    59761 ggcaaccatt tgaacccacg cctgctgaac gacggcatct atcatagctt ccgccagcca
    59821 ggttaagtga tcgctcactt tcattaccgg tagcgtaccg gcgatatccg ctgccgcaat
    59881 gcgtaacagt tgcgcctgtt tgaactgacg cagcgcctca agctgttgtt cttcgtcatc
    59941 ttccggcacg cgcagcaaat actggcgcag ttcatcacgg taggcatcgg tcgccgtcgg
    60001 ctggtagaga gtgtttggat cgagcagttc atccagcaat aacggatagc gcgccagctg
    60061 gctggcaatc atcggcgatg cggcacacag agaaatcaaa tgcttaagcg ccgcaggaaa
    60121 ttcactgagc aattctaaat aggtggtgcg ggtaacaatc cccaccagca aggccgtaat
    60181 gcgcgacagc gttacagcag cgtcttcacg tgtacagaca tctcttagca gatgtggcat
    60241 cagatggtcg agcacctgac gtccgcgtgg cccgattgtg cgcttatcca gctctttgcg
    60301 gaaatcggca atcagcgtca gcacctgttt gcgatcgtcc tcgctaagat gcgccagcac
    60361 tggtgtagtg tcatcttcct gcaacgcgtc ctgccacagc tcacgccact gttccgacag
    60421 cgactcttcc tgagtttcac tttcgtcgtc gccaatcaat tcattaaaca cccggcgcac
    60481 attggtcata tgtccgatca gcgccccagt cagttgctgc cagtcagcaa agtccatcgc
    60541 ccacgccagc cgcgcacggt taagctcatc agaaggaagc gtctgggttt gttcgtcgtt
    60601 aatgctttgc agcaggtttt ccagacgccg caggaacaga tacgccactc gcaattgttc
    60661 agcatcgttt tcagaaagca gatgcagctc ggcaatggcg ctgagcgttg gcagtaaaga
    60721 gcgcgattgc agcgacggtt cgcgcccgcc gcgaatgagc tggaatacct ggacgataaa
    60781 ttcaatttca cgaatgccac ccgcgccaag tttgatgttg tcggtcaaac cgcgccgacg
    60841 cacttcacgg gcaatcatcc ctttcatgtt gcgcagcgac tgaatcacgc tgaaatcgat
    60901 gtaacgacgg aaaacaaacg ggcgcagcat cgcacgcaac tcgttagcat agacgccttc
    60961 gctatcgccc ataatccgcg ccttgaccat cgcgtaacgc tcccagtcgc gcccctgctc
    61021 ctggtaataa tcttccagtg ccgcaaagct cagcaccagc gggccacttt cgccaaacgg
    61081 acgcagtcgc atatccacgc gatagacgaa gccatcctgc gttggttgat ccagcacttt
    61141 aatcagccgc tgccccatgc gggtaaaaaa ctgggcgtta tccagttccc ggcgtccgcc
    61201 ctgcgtacaa ccatgttccg gccaggcaaa aatcagatcg atatctgagg agaaattcag
    61261 ttcccctccg cccagcttac ccatgcctaa aatcagcagc ggttgcgctt cgccctgcgc
    61321 attgcacggc gttccccact cgcggcagca ggcgtcatac agccagtcac gcgccgcaac
    61381 aatcagcgtc tccgccagat ggctgagctg cagcaatatg ctctcttcag taaccagtgc
    61441 caacgtttgc gcccaggcga tgcgcaccat aatgcgccgc cggaatagcc gcagctcgcg
    61501 cattaacccg gcttcgtcac tcacattact gagcgcctcc tgccaccatg cagagtaatg
    61561 ctgccattcg tcggcctgcg gcggttggct ttccagttcc gtcagccact ctggatgcgc
    61621 aatcacgctg tcctgcacaa aatcactgaa agtaagtacc gacttcgcct gtgcgctaag
    61681 tgattcgtcg gctaaaggct ctggcagccg ctcaacaacg gtctgccagt actgctgtaa
    61741 cggtgaagag agcggcttca taagcgattt tatccttgcc tgattaacgt tttccgctgt
    61801 gcaaccagaa cggttcctga ttgtttgcct cattacggaa atgttcaatt tcaatgcgtt
    61861 gcccggtcac aatagcgtga cgcagcccct gccagttctc cagccaggct tgagccacga
    61921 caggatcata gtaacccgcc agcaacagta ttgagtcaat atcacgcgtc agacgtggca
    61981 actggtcacg gtagcgatcg cctaacggct ggcagaaaac gcttttcagt tcagcggcat
    62041 ggcgggaaag atggatatcg gcaaagcgtt tgaaggaatc gctcatttta ctctgcgctt
    62101 tggcatctaa aaacggctgc caggctttgc ttaccagcca ttcggtcaac gccagcttcg
    62161 ccattgccgt ttcggtagaa tagaccgccg tcacggcaga aaccactgaa gcgatggtcg
    62221 cttcgcattg ggtcagcaaa tcacgtaagt gagtgctcgc tttacgcggc acaatgccac
    62281 cgaacagcat cagcgtatga cggaccaggc caatggctgc cagcacctgc tctttcgccg
    62341 catcgttgcc gcgtacccac agctcttcat gatactgcca ttgcgctaac gccagctcga
    62401 gcgccgcttc cagcccctgc tcgacatccg cttttgccgc aacatgtaaa atggtggtcg
    62461 gtttgatttc acgcgccgga ttgccctgcg ccagatgata gccacgcgcc gctttgctca
    62521 ggctgccctg gcgtaatccg gtttgcgata ccagttggtt cgccagtttc agcaccgcgc
    62581 gcgtgtcgcc gctaagcagt tccagttcca gctcgcagat aggttcagca aattcacccg
    62641 ctttcacttc cccttggtcg agggcgattt caatttggct accatcaacc tctaccagcc
    62701 acttttcgcg ataaaaatcg gtgctaaaca gcggctgcac gcgggaagcg agatcggtag
    62761 gcaattcgcc gttcggccag acttccgtcg gtaactgcgc caggtcgagc atcggttcgc
    62821 tcaacgccac gttatattcc gggcgctggt gtaagccgcc tgtcactctt cctgcaactt
    62881 tcatggtcat ctcatagcga ccgttttcgc cacgaatacg caagcccatg tcgtgcccac
    62941 gcagccagtt atccggcgtt tcgtagtaaa tattcagcaa ctgcacgggg tcatggtgct
    63001 cgccgcccag cgtattgaga tggtcacgca acgcctcaac ggcactgtga ttaacaataa
    63061 actttaattc gatttcctga gccatagcct ttacttatcg gttatgtcac ataagtgacg
    63121 atgaacggcg aacttaatgc gatctttttt gtcagtagat agtattttgc gccaaattgc
    63181 catgcaacgg gcaatttgac gggcgtaaaa gtttgaagca gtggcaagac ctgacacaga
    63241 ggattccgat tgatgtcgaa tgctttgcgt agcgacactg atgccactac tatcgttcca
    63301 ctttccatga caataacgac agcctgatgc caaaattacg cctgatcgga ttaactttac
    63361 tcgcacttag cgcgactgcc gtctcacacg ctgaagaaac gcgctatgtt tctgacgaac
    63421 tgaatacctg ggtccgtagc ggtccgggag atcattatcg cctcgtaggc acggttaacg
    63481 ccggcgagga agtgacctta ttacaaactg acgccaacac caattatgcc caggtgaaag
    63541 acagctctgg ccgtaccgcc tggatcccgt tgaaacaact tagcactgag ccaagcctgc
    63601 gctcccgtgt gccagatctg gaaaatcagg tcaaaaccct gaccgataaa ctcaccaata
    63661 tcgataacac ctggaatcag cgcaccgcag aaatgcagca aaaagtggcg cagagcgaca
    63721 gcgtgatcaa cgggttaaaa gaagaaaatc agaaactgaa aaacgagctg attgtcgcgc
    63781 agaaaaaggt cgatgccgcc agcgtacagc tggatgacaa acagcgcacc atcatcatgc
    63841 aatggtttat gtatggcggc ggcgtgctgg ggcttggctt gctgctcggt ctggtactgc
    63901 cgcacctgat cccaagccgc aaacgcaaag atcgctggat gaactaaatc gccttcttcg
    63961 ttgcactgtc ttattattag gaattgtgta tttgacgaag ggatgatggc gtgaagattt
    64021 atctggtcgg tggtgctgtt cgggatgcat tgttagggct accggtcaaa gacagagatt
    64081 gggtggtggt cggcagtacg ccacaggaga tgctcgacgc gggctaccag caggtaggcc
    64141 gcgattttcc tgtgtttctg catccgcaaa cgcatgaaga gtatgcactg gcacgtaccg
    64201 aacggaaatc cggttccggt tacaccggtt ttacctgcta tgccgcaccg gatgtcacgc
    64261 tggaagatga tcttaagcgt cgcgatctga ccattaatgc gctggcccag gacgataacg
    64321 gcgagattat cgacccgtac aacggtctgg gcgatctgca aaatcgtctg ttgcgccatg
    64381 tttcccccgc ttttggcgaa gatccgttac gcgtgttgcg cgtagcgcgt tttgctgcgc
    64441 gttatgccca cctcggtttt cgtattgccg atgaaactct ggcgctgatg cgcgagatga
    64501 cccacgcggg tgaactggaa cacctgacgc ctgaacgggt atggaaagag acggaaagcg
    64561 ccctcactac ccgcaatccc caggtgttct tccaggtact gcgcgattgc ggcgcactgc
    64621 gcgttttatt cccggaaatt gacgccctgt ttggcgttcc ggcccctgcc aggtggcatc
    64681 cggaaatcga cacgggtatt cataccttaa tgacgctctc aatggcggcg atgctgagtc
    64741 cgcaggtcga tgtccgtttc gcaacgttat gccacgatct cggtaaagga ctgacgccac
    64801 cagaactttg gccgcgtcat catggtcatg gcccggcggg tgtgaagtta gtggaacaac
    64861 tatgccagcg tctgcgcgtg ccaaatgaaa ttcgcgattt agccagactg gtggctgagt
    64921 ttcacgatct catccacacc ttcccaatgc tgaacccaaa aaccatcgtc aaactatttg
    64981 attccatcga cgcctggcgt aaaccgcagc gtgtcgagca actggcgctg accagcgagg
    65041 ctgatgtgcg cggcagaacc ggttttgaat cagccgacta cccgcaaggc cgctggttgc
    65101 gcgaagcctg ggaagtggca cagtcagtgc cgacaaaagc cgtcgttgaa gcgggattta
    65161 aaggtgtgga gattcgcgag gagctgaccc gacggcggat tgcggcggta gccagctgga
    65221 aggaacaacg ttgtccaaag cctgattaag cacttgaatt tcaatttatt gcaaacaccg
    65281 gccaggtcgg ataagacgca caagcgtcat atccgacact attcctcaat caaaagaaca
    65341 cgacatacac cgcagccgcc acaataaagc gataaatggc gaaagggata aacgaaatgc
    65401 gcttaatcaa ttgcaggaag gttttaatcg ctatcagcgc caccacaaaa gcggtgataa
    65461 acccaacggc aaacatcggg atatcgccgg tagtcaggaa gccccagctt ttgtagagat
    65521 cgagcgccgt tgcgcccatc atcatcggca ccgccagcag gaacgagaac tcggaagcag
    65581 cgtaacggct cacgcccatc agcatcccac ctgaaatggt cgccccggag cgagagaaac
    65641 ccggccacag cgccagacac tggaaacagc caatcataaa cgcctgacga taggtcatat
    65701 catcaagacc cggcgcacgc ggctctttcg gcttcaggca ttcagcggca atcagcaaca
    65761 gaccgccaac gaccagtgca tacatcacat ttatcgggtt aaacaatgat ttaatcgtgt
    65821 cgtggaacaa cagccccagt accaccgccg gaatcatccc cagcaaaatg tggatcagcg
    65881 ttaaacggcc tttgctttct ccttcgtgct gcaacgggcg accaaagtga ataccaataa
    65941 ggccaaacag acgtcgccag aacatgacaa ccactgccag aattgatcct aactggatca
    66001 cgacttcaaa ggttttcgcc gtgtcgccct caaaccccaa caagtgaccg acaataatca
    66061 tatggcccgt gctggatacc ggcagaaatt ctgtcaatcc ttcgaccaca cccaatattg
    66121 ccgctatcag cagcgagtgc atatcgctca tcaataaacc cctaaattat taaaatgtac
    66181 cgcttgtccg aactactgca gtatgaccag gttataaccg tttggtttaa cagctgtaaa
    66241 attaattatt ttctttcaga ttattgccac gctcaatgat gacgccgaca ttcgccgctc
    66301 gcgccaccgc acctagcttg ctaagtttga tgcgcaccca cggcgagttg aagcgtgcta
    66361 acagcagctc cgccacctct tcagccacgc gttccaccag cgcaaaacgc gccccctcga
    66421 cgtggctgac caccgtttct gcaatgtcag cgtaactgag gcaatccgcc acatcatcac
    66481 ttttcgccgc tttacggtta tcccacgcca tttcgatatc gaacactaac ttctgttcga
    66541 tggtctgttc ccagtcgtaa acaccaatag tggtgattac cgaaagttgc tctataaata
    66601 caatatccat cacgtcctgc ctgctttttg gctaaccgga taccacttcc ggcgaaatgt
    66661 gcgtattatc cacagattca tcgttgaaca cgaattttca aaacggaaca gcttatgagt
    66721 gcaatcgcgc ctggaatgat cctcatcgcg tacctctgcg gctccatttc cagtgccatt
    66781 ctggtttgcc gcttgtgtgg gttgcccgat ccgcgaacca gcggctccgg caatccaggc
    66841 gcaaccaatg tgttacgtat cggtggcaag ggagcagccg tagcagtact gattttcgac
    66901 gttctgaaag gaatgttgcc tgtctggggc gcgtatgaat taggtgtcag ccccttctgg
    66961 ctaggcttaa ttgccatcgc cgcctgtctt ggacacatct ggcccgtttt cttcggtttt
    67021 aaaggaggaa aaggcgttgc taccgctttt ggtgccatcg caccaattgg ctgggatctc
    67081 accggagtaa tggcgggaac ctggttactg accgtgctat tgagcggata ttcgtcgctg
    67141 ggagcgattg tcagtgcact gattgctccg ttttatgtct ggtggtttaa gccacaattc
    67201 accttcccgg tttcgatgct ctcttgcctg atcctgctgc gtcatcatga caacatccag
    67261 cgtctgtggc gtcgtcagga gacaaaaatc tggacgaaat tcaaaagaaa gcgcgaaaag
    67321 gatcccgagt gatttctggt ggatttacat gacctgatag ccttcgtcgg gcttgcccag
    67381 ccgttgctgg caccatgccg ccagaaattc cacgcagaca cgtaatttca tgctgcgata
    67441 gagcggctcc cggtaaacag cccagatatt ggcgctttgt gcatactccg gcaatactcg
    67501 caccagtttg ccactctcca gaaacggcaa cacatcccac tcggaacgca acataatccc
    67561 tttgccctcc agcgcccatt gcaacactat ctcgccgcta ttggaggaaa gatgcccgct
    67621 taccttcacc gattttttct cctgaccgtt ccccaactcc catattccgt gggtcatatc
    67681 gcgctctttg gtcaccagac aatcatgacg acttaattcc tgtaaggatt gtggctgcgg
    67741 atatttttgc agatactcag gtgctgcgca taatattctt ttatttttcg ttaacagatg
    67801 cgcaatataa taatcaggaa tttcgtcatt aatacgaata tccagatcaa tattatcctg
    67861 cactaaatca atttgccgat cgaacaattc aaaatgcacc tgtaactcag gataattgcg
    67921 catcagttcg gtaatggctg gcgcaatatg gctgcgccca aaaccaaaac tgcaaccaat
    67981 acgaatcatc ccctccgggc gcgttttgat ttgcgtgacg tcatcgacca gtcgctgata
    68041 ttgggtgagg atttccagcg catgctcgta acaacgttgg ccactttcag tcagcgccac
    68101 gccgcgagcg gagcggttca gcagcgttgt tgccagcgta ttttcaagga tttggatccg
    68161 cttggtgaca aatgcaggcg tctgcccaag cgtcgccgct gcggcgctaa aactcccgct
    68221 gtggacgatt tctaccagta cctgcaaatc tttggctaaa ggccagctat tcagcatcgt
    68281 taattatccg cagttgtgat aagcgcagtg tatttcgtaa aacattgcca ttccctgctc
    68341 tggcgcaata ttcattctta ttttcgcgtt gattcacgaa ctgtgaatta catattcacg
    68401 gcagcaaaaa atataaaacc acatttttag tggtagtttg tggcggtgaa tttttccaga
    68461 caaatacata aactggagtt gccatgatga gcgaaagtaa taagcaacag gcagtgaata
    68521 agttgacgga gattgtcgct aactttaccg ccatgatttc tacccgaatg cccgatgacg
    68581 tggtggacaa actaaaacag ctaaaggatg ccgaaacatc gtcgatgggg aaaatcatct
    68641 accacacgat gttcgataac atgcaaaaag cgatcgacct gaatcgtcct gcctgtcagg
    68701 acaccggcga aatcatgttt tttgttaagg tcggttcccg tttcccactg cttggcgagc
    68761 tgcaaagcat actcaaacaa gccgtggaag aggcaaccgt caaagcgcca ctgcgtcaca
    68821 atgcggtaga aatttttgac gaagtaaaca ccggcaaaaa taccggcagc ggtgtaccgt
    68881 gggtcacctg ggacatcatc cccgacaatg acgatgcgga aatcgaagtt tacatggcag
    68941 gcggcggctg cacgctacca ggccgctcga aagtgttaat gccgtcagaa ggctacgaag
    69001 gcgtagtgaa attcgtcttc gaaaatatct ccaccctcgc cgtaaacgcc tgtccgccgg
    69061 tacttgtggg cgttggcatc gctacctcgg tggaaaccgc cgccgtgctc tcgcgtaaag
    69121 ccattttgcg cccgattggc agccgccacc ccaatccgaa agcggcagag ctggagctac
    69181 gcctggaaga aggactcaac cgtctgggga ttggtccaca agggctgact ggcaacagtt
    69241 cagtgatggg cgtacatatc gaatctgccg cccgccatcc gtcaaccatc ggcgttgctg
    69301 tttctacagg ctgctgggcg catcgtcgcg gcacactgct ggttcatgcc gatctcacct
    69361 ttgaaaatct gtctcacacc cggagcgcgt tatgaaaaag atcctgacaa ccccgatcaa
    69421 agctgaagat ctgcaagata ttcgcgtcgg cgatgtgatc tacctgaccg gtacgctggt
    69481 gacctgccgt gacgtttgtc accgtcgctt gatcgaactc aaacgtccga tcccttacga
    69541 tctcaacggc aaagcgattt tccacgctgg ccccatcgtg cgtaaaaacg gcgacaaatg
    69601 ggagatggta tccgtcggcc cgacaaccag tatgcgtatg gaaagttttg aacgtgaatt
    69661 tatcgaacag accggcgtga aactggtggt cggcaaaggc ggaatggggc cgctgaccga
    69721 agaaggctgc cagaaattca aggcgctaca tgtgattttc ccggcaggct gcgcagtact
    69781 ggcggcaacc caggtggaag agattgaaga agtgcactgg acagaactcg ggatgccgga
    69841 gtcactgtgg gtctgccggg tcaaagagtt cggcccgctg attgtctcta ttgataccca
    69901 cggcaacaac ctgatagccg aaaacaaaaa gctgttcgcc gaacgccgcg atcccatcgt
    69961 ggaagagatc tgcgagcacg tccattacat caaataaccc tcccggagag gctcgcccct
    70021 ctcctttttc gcaggcataa cacgatgaaa ccttccactg aatggtggcg ataccttgcg
    70081 ccgctggcgg tcatcgccat tattgctcta attccggttc ccgcagggct ggagagtcat
    70141 acctggctct actttgctgt ttttactggc gtgatcgttg gactgatcct cgaacccgtg
    70201 ccgggtgccg tggtggcgat ggtgggtatc tccattatcg ccattctctc tccctggctg
    70261 ctgttcagcc cggagcagct cgcccagcca ggttttaagt tcaccgccaa atccctctcc
    70321 tgggcggttt ctggtttttc taattcggtt atctggctga ttttcgccgc ctttatgttt
    70381 ggcacaggct atgaaaaaac cgggctgggg cgacgtatcg cgctgattct ggtgaaaaag
    70441 atggggcatc gcacgctgtt tctcggctat gcggtgatgt tctccgagct tatcctggca
    70501 cctgtaacac cgtccaactc ggcgcgtggt gcggggatta tctatcccat catccgtaac
    70561 ctgccaccgc tctatcaatc acaaccaaac gacagcagtt cgcgcagcat tggctcgtac
    70621 atcatgtgga tggggattgt tgccgactgc gtgaccagcg ccattttcct gacggcgatg
    70681 gcacctaact tgctgttaat tggcctgatg aaaagcgcat ctcacgccac actgagttgg
    70741 ggcgactggt tcctcgggat gttgccgctc agtattttac tggttctgct ggttccctgg
    70801 ctggcttacg tgctgtaccc gccggtactg aagtctggtg atcaggtgcc gcgctgggca
    70861 gagacggaac tgcaggcaat gggcccgctc tgttcgcgtg aaaaacggat gctggggctg
    70921 atggtaggcg cgctggtgct gtggattttc ggcggtgatt atatcgatgc cgcgatggtc
    70981 ggttacagcg tggtggcgct gatgctgctt ctgcgcatta tcagttggga cgacattgtc
    71041 agtaataaag ccgcgtggaa cgttttcttc tggctggcct cgcttatcac cctcgctacc
    71101 ggactcaaca acaccggttt tattagctgg tttggcaaac tgttagcagg cagcttaagc
    71161 ggttattcgc caacgatagt gatggtggcg ttgattgtgg tgttttatct actgcgctac
    71221 tttttcgcca gcgccacggc gtatacctcc gctctcgcgc cgatgatgat tgccgccgcg
    71281 ctggcgatgc cggaaatccc gctgccggta ttctgcctga tggttggcgc ggcaattggt
    71341 ctggggagca ttcttacgcc atacgccacc ggacccagcc cgatttacta cggtagtggt
    71401 tatctgccaa cggtggatta ctggcgactg ggggcaattt ttgggctgat attcctcgta
    71461 ttgctggtga ttaccggctt actgtggatg cccgtggtgt tgctttaagt gaggacggtg
    71521 ccggaagtga ctctccggca cctaatagtt ttacgcggcc ggtaactctg ccagcggcca
    71581 gcgcggacgc acgctaacgc cgagatccgc cgtcgcgcct gctttaaacc gcaccattcc
    71641 ggcataggcg atcatcgcgc cgttatcagt acaaaattcc ggacgcgcgt agaacacttc
    71701 gccacggcgt tttttcatca tttcagccag cttcgcccgt aacgtgcggt tagcactcac
    71761 gccgcctgcc attaccagtc gcttaaagcc cgtctgatcc agtgctcgct tacacttaat
    71821 catcaacgta tcgaccaccg catcttcaaa ggcgcgggcg atatcagcac gcgtctggtc
    71881 gtcggtgccg ttgtcacgaa tcgtgttcgc cgcgaaggtt ttcagaccag aaaagctgaa
    71941 atccagcccc ggacggtcgg tcatcgggcg cgggaagaca aagcgcccgg cagtaccctg
    72001 agccgccatt ttcgacagta atggcccgcc aggataatcc agccccagca gcttcgcggt
    72061 tttatcaaac gcttcaccag cagcatcatc gatagactcg ccaagcagct cgtactgacc
    72121 aatgccagtc acgctgatta actgcgtatg accgccggaa accagcagcg ccacaaacgg
    72181 aaatgccggt gggttatctt ccagcatcgg cgctaacaga tgcccttcca tatggtgtac
    72241 cgggattgcc ggaacgtccc aggcaaacgc cagagaacgc cccacggtcg cgccaaccag
    72301 cagtgcgccg actaatcctg ggcctgcggt ataggccaca gcatcaatgt cttttgccgt
    72361 taaaccagac tctttcagcg cctcctggat caacggtacg gttttacgca cgtgatcgcg
    72421 ggaggccagt tcaggcacga cgccgccgta gtcagcgtgc aatttcacct gactatacaa
    72481 ttggttggct aacaaacctt tttcatcgtc gtaaatggcg atgccggttt catcgcagga
    72541 agtttcaata cccagtacac gcatgacttg ttttacctcg ctttattacc gcgcagtgta
    72601 ggaccaatgc gggttgatgt aaaactttgt tcgcccctgg agaaagcctc gtgtatactc
    72661 ctcaccctta taaaagtccc tttcaaaaaa ggccgcggtg ctttacaaag cagcagcaat
    72721 tgcagtaaaa ttccgcacca ttttgaaata agctggcgtt gatgccagcg gcaaaccgaa
    72781 ttaatcaaag gtgagaggca catgccggta attaaagtac gtgaaaacga gccgttcgac
    72841 gtagctctgc gtcgcttcaa gcgttcctgc gaaaaagcag gtgttctggc ggaagttcgt
    72901 cgtcgtgagt tctatgaaaa accgactacc gaacgtaagc gcgctaaagc ttctgcagtg
    72961 aaacgtcacg cgaagaaact ggctcgcgaa aacgcacgcc gcactcgtct gtactaattc
    73021 cccgagagcg ttgctctccg atcagaccga gttgtagttg taaggccgtg cttccgaaag
    73081 gaatgcgcgg cttattttcg tttatgaatt gctaaaaatc ggggcctatg gctggacgaa
    73141 tcccacgcgt attcattaat gatctgctgg cacgcactga catcgtcgat ctgatcgatg
    73201 cccgtgtgaa gctgaaaaag cagggcaaga atttccacgc gtgttgtcca ttccacaacg
    73261 agaaaacccc atcattcacc gttaacggtg agaaacagtt ttaccactgc tttggatgtg
    73321 gcgcgcacgg caacgcgatc gacttcctga tgaactacga caagcttgag ttcgtcgaaa
    73381 cggtcgaaga gctggcagca atgcacaatc ttgaagtgcc atttgaagca ggtagcggcc
    73441 ccagccagat cgagcgccat caacgacaaa cgctttatca gttgatggac ggtctgaata
    73501 cgttttacca acaatcttta cagcaacctg ttgccacgtc tgcgcgccag tatctggaaa
    73561 aacgcggatt aagccacgag gttatcgccc gctttgcgat tggttttgcc ccccctggct
    73621 gggacaacgt cctgaagcgg tttggcggca atccagaaaa tcgccagtca ttgattgatg
    73681 cgggcatgtt ggtcactaac gatcaggggc gcagttacga ccgtttccgc gagcgggtga
    73741 tgttccccat tcgtgataaa cgcggtcggg tgattggttt tggcggacgc gtgctgggca
    73801 acgatacccc caaatacctg aactcgccgg aaacggacat tttccataaa ggccgccagc
    73861 tttacggtct ttatgaagcg cagcaggata acgctgaacc caatcgtctg cttgtggtcg
    73921 aaggctatat ggacgtggtg gcgctggcgc aatacggcat taattacgcc gttgcgtcgt
    73981 taggtacgtc aaccactgcc gatcacatac aactgttgtt ccgcgcgacc aacaatgtca
    74041 tttgctgtta tgacggcgac cgtgcaggcc gcgatgccgc ctggcgagcg ctggaaacgg
    74101 cgctgcctta catgacagac ggtcgtcagc tacgctttat gtttttgcct gatggcgaag
    74161 accctgacac gctggtacga aaagaaggta aagaagcgtt tgaagcgcgg atggagcagg
    74221 cgatgccact ctccgcattt ctgtttaaca gtctgatgcc gcaagttgat ctgagtaccc
    74281 ctgacgggcg cgcacgtttg agtacgctgg cactgccatt gatatcgcaa gtgccgggcg
    74341 aaacgctgcg aatatatctt cgtcaggaat taggcaacaa attaggcata cttgatgaca
    74401 gccagcttga acgattaatg ccaaaagcgg cagagagcgg cgtttctcgc cctgttccgc
    74461 agctaaaacg cacgaccatg cgtatactta tagggttgct ggtgcaaaat ccagaattag
    74521 cgacgttggt cccgccgctt gagaatctgg atgaaaataa gctccctgga cttggcttat
    74581 tcagagaact ggtcaacact tgtctctccc agccaggtct gaccaccggg caacttttag
    74641 agcactatcg tggtacaaat aatgctgcca cccttgaaaa actgtcgatg tgggacgata
    74701 tagcagataa gaatattgct gagcaaacct tcaccgactc actcaaccat atgtttgatt
    74761 cgctgcttga actgcgccag gaagagttaa tcgctcgtga gcgcacgcat ggtttaagca
    74821 acgaagaacg cctggagctc tggacattaa accaggagct ggcgaaaaag tgatttaacg
    74881 gcttaagtgc cgaagagcga tcgggaagcc cccgacagcc gcactgagag gcagcggcaa
    74941 atatataagt acgccctcgt aattatcgtt ggcggtaaac aaccgttgga tttcagcgtt
    75001 aacagctgaa ggacatcggg tcaatcgccc aacaccaacc tcatgaaata agtgtggata
    75061 ccgtcttatg gagcaaaacc cgcagtcaca gctgaaactt cttgtcaccc gtggtaagga
    75121 gcaaggctat ctgacctatg ccgaggtcaa tgaccatctg ccggaagata tcgtcgattc
    75181 agatcagatc gaagacatca tccaaatgat caacgacatg ggcattcagg tgatggaaga
    75241 agcaccggat gccgatgatc tgatgctggc tgaaaacacc gcggacgaag atgctgccga
    75301 agccgccgcg caggtgcttt ccagcgtgga atctgaaatc gggcgcacga ctgacccggt
    75361 acgcatgtac atgcgtgaaa tgggcaccgt tgaactgttg acccgcgaag gcgaaattga
    75421 catcgctaag cgtattgaag acgggatcaa ccaggttcaa tgctccgttg ctgaatatcc
    75481 ggaagcgatc acctatctgc tggaacagta cgatcgtgtt gaagcagaag aagcgcgtct
    75541 gtccgatctg atcaccggct ttgttgaccc gaacgcagaa gaagatctgg cacctaccgc
    75601 cactcacgtc ggttctgagc tttcccagga agatctggac gatgacgaag atgaagacga
    75661 agaagatggc gatgacgaca gcgccgatga tgacaacagc atcgacccgg aactggctcg
    75721 cgaaaaattt gcggaactgc gcgctcagta cgttgtaacg cgtgacacca tcaaagcgaa
    75781 aggtcgcagt cacgctgctg ctcaggaaga gatcctgaaa ctgtctgaag tatttaaaca
    75841 gttccgcctg gtgccgaagc agtttgacta cctggtcaac agcatgcgcg tcatgatgga
    75901 ccgcgttcgt acgcaagaac gtctgatcat gaagctctgc gttgagcagt gcaaaatgcc
    75961 gaagaaaaac ttcattaccc tgtttaccgg caacgaaacc agcgatacct ggttcaacgc
    76021 ggcaattgcg atgaacaagc cgtggtcgga aaaactgcac gatgtctctg aagaagtgca
    76081 tcgcgccctg cagaaactgc agcagattga agaagaaacc ggcctgacca tcgagcaggt
    76141 taaagatatc aaccgtcgta tgtccatcgg tgaagcaaaa gcccgccgtg cgaagaaaga
    76201 gatggttgaa gcgaacttac gtctggttat ttctatcgcc aagaaataca ccaaccgtgg
    76261 cttgcagttc cttgacctga ttcaggaagg taacatcggc ctgatgaaag cggttgataa
    76321 attcgaatac cgccgtggtt acaagttctc cacctacgca acctggtgga tccgtcaggc
    76381 gatcacccgt tctatcgcgg atcaggcgcg caccatccgt attccggtgc atatgattga
    76441 gactatcaac aaactcaacc gtatttctcg ccagatgctg caagagatgg gccgtgaacc
    76501 gacgccggaa gaactggctg aacgtatgct gatgccggaa gacaagatcc gcaaagtgct
    76561 gaagatcgcc aaagagccaa tctccatgga aacgccgatc ggtgatgatg aagattcgca
    76621 tctgggggat ttcatcgagg ataccaccct cgagctgccg ctggattctg cgaccaccga
    76681 aagcctgcgt gcggcaacgc acgacgtgct ggctggcctg accgcgcgtg aagcgaaagt
    76741 tctgcgtatg cgtttcggta tcgatatgaa caccgaccac acgctggaag aagtgggtaa
    76801 acagttcgac gttacccgcg aacgtatccg tcagatcgaa gcgaaggcgc tgcgcaaact
    76861 gcgtcacccg agccgttctg aagtgctgcg tagcttcctg gacgattaat tgtaaagatg
    76921 tgcataagcc ctccatcgtg agggcttttt tttaaggaaa tctggcaatg cagcaaggat
    76981 actactctct ccccgccctt atttccgctc tcgatgccgg gaaaaaggtg aaatacctgt
    77041 ggttctgggg ccatcaacct gccgcgaatc acgaaattac cgcctcttgc tttagccagt
    77101 ggtggcaggg ttcgcctttt acctatgatg gtataaccta tgccaccgca gaacactgga
    77161 tgatggcggg aaaagcgcgg ctgtttaatg acagtaaaac acttgccaga attctggcgg
    77221 cggcgacccc ggcagaagcc aaaaagctgg ggcgacaggt gacaaacttt gacgagcaat
    77281 gctggcaagc ttgctgcttt gatctggtct gcgaagggaa ctaccataaa tttagccaac
    77341 attcggcatt aaaaacattc ctgctgaata cgcagcctcg cattctggtt gaagccagcc
    77401 cggtggataa aatctggggg attggccttg caaaagatca tccccatgcc ggtgtgccat
    77461 ctcactggaa ggggaaaaat ctgctgggat ttgcgcttat ggtagtcaga gaccgtttgg
    77521 cggaagaata agccagtagc ggtgaggagc ggtgcctggc ccgctcataa cttcgttaca
    77581 ctgtacttca caagccacgc actaccagcg cctggtccag ctcgcgatac gcttcaacca
    77641 gtttctccag tgaaacgcga cttaaaccgc tgggatttgg cagcacccaa atctgcgtcg
    77701 aaccaatggt gagcgtttgt ttcccccact gtgcaccgcg ctggctgaat ccctgttcat
    77761 atgcttgttt gcccagaatc gccaacgcct gcggctgata atcttctatt ttttcaatta
    77821 gcttacgccc gcctgcgtgc agctcctgct ttgaaacttc attggcttgc accgttggac
    77881 ggtctaccag tttggtgacg ccacaacgat aatccagcag atgctgtgcc tcctgcggct
    77941 tcaactgacg gtcggtaaac ccggcctgat atatcacctt ccagaagcga tttgccggat
    78001 gagcaaaggg aaaaccagtc ccggcggatg aaagcccagg gttgataccg caaaacacga
    78061 cccgtaaccc tggagccaaa atatcctcaa ccatatttac tcctttctaa catcaccagg
    78121 aaaagtataa cggattgaaa atacattgtt tataaaaaca gcaggcgcgc ggtaatggct
    78181 ggattgcgac acggagttac tttataatcc gctaccatgg ccccttagct cagtggttag
    78241 agcaggcgac tcataatcgc ttggtcgctg gttcaagtcc agcaggggcc accagacata
    78301 gcaaaggctg acgagaaatc gtcagccttt ttctttatat atatcagtta ctttgcgtgc
    78361 cagtaagccg ctgcacgtac ccgctgtggg tcatactgtt ccgcttcaaa gcggcggctt
    78421 aaattcttaa cgactttgcc ttcgccggtt atccagatga agtaatcatc ggcagggatt
    78481 tgcatctgcg ccagacgcgc atctaccgcc tgctcatcat gtgccagcca ttcgatatta
    78541 aaaccatcaa ggtgcgcgag ataatcctga caggcgttat cccgcacgct aaccagcgca
    78601 ctaacttgcg gtttaacggc aagtttgctc aacgtttcca ggcggcggcg cagtgcgggc
    78661 attccggatt catcgcagac atacagctga tacgcgtaat cttccggcac caccagcgaa
    78721 ccgcgcggac ctgccaccgt aagtttatcg cccggtttcg cctgcatcgc ccagccgctg
    78781 gcgaccccac cgtcgtgaat aaagaaatcg atcgccagtt catggcgtag ttcgtcatac
    78841 agcggcgtat agtcacgcga cggtgggcgt ggtccttccg gccagacgat gccctcttcc
    78901 gttaccgttg gcggcacaaa gtgagcgtca ggttgaggaa agaagagttt gctgtgatcg
    78961 tcaaagccgc gcgacgtaaa accgtccagc gcctcgccgc cgaggacaat gcgctgaaaa
    79021 ccggcgctga tgcgctcaac gcgtaacaca gtcagttcac ggaagcgcag atcattgcga
    79081 acgcgctgcg ggtagcgggg ggagttattc attgttatcg ccttcgtgat agtaatcaga
    79141 tatatctaaa taattttcgt aaatgataat gattgttaat catgataaat gcaagcgatt
    79201 tgtagaactg atatatctgt agtctgataa gacgcgcaac ctcttatcaa gcatcattgc
    79261 tcaatgtcgg atgcggcgtg aacgccttat ccggcctacg tgtgagatga gccacattta
    79321 aaaatcattt ttattcttgc aagagtgctc atatcagata taaattagat atatctaatt
    79381 aagcaaaagg aggctgatat gagccatcat cacgaagggt gttgtaaaca tgaaggccag
    79441 ccacgccatg agggctgctg caaaggtgag aagtcagaac acgagcactg cggacacggt
    79501 caccagcatg aacacggtca atgctgcggt ggtcgccacg gtcgcggcgg cggtcgtcgg
    79561 caacgtttct ttggtcacgg tgaattacgt ctggtgattc tggatattct ctcgcgcgat
    79621 gacagccacg gttacgaatt gattaaagcg attgagaatc taacccaggg gaattacacc
    79681 ccaagcccgg gcgtcatcta cccaacgctg gattttctgc aggagcagtc gctgattacc
    79741 attcgcgaag aggaaggtgg taagaagcag attgcgctga ccgaacaagg cgcgcagtgg
    79801 ctggaagaaa accgcgaaca ggtagagatg attgaagaac gcatcaaatc gcgttgcgtt
    79861 ggcgctgcgc tgcgccagaa cccgcaaatg aagcgggcgc tggataattt taaagcggtg
    79921 ctggatttac gcgtcaacca gagcgatatc actgatgcac aaataaaaaa gatcattgcg
    79981 gtgatcgacc gcgccgcttt tgatattacg caactggatt aatcaccgat tgccggatcc
    80041 ggcataccga ttaatgcagc accgtcaccg cgtcttccag tcggctggcg cggtgtttca
    80101 ccatcgccga cacctgcgca ctctcttcca ctaactcggc gtttttctgg gtgatcaggt
    80161 taagctcatc cactgcacgg gtcaggctgg aaagcccatc ggcctgttcc agcgttgaat
    80221 ggctaatctg agctatcaac tgggtgacgt tttttacctg cgccacaata tcttccatcg
    80281 tccgtccggc ggcgtgtacc tgctgcgaac cagattgcac cttatcagca ctggcatcaa
    80341 tcagcttgcg aatatcgttg gcagcattgg cgctgcggct ggctaaatga cgcacttccc
    80401 ccgccaccac tgcaaaacct ttgccctgtt cgccggcacg cgccgcttcc accgccgcat
    80461 tcagggccag aatattggtc tgaaacgcaa tatcgttaat cagcgaagta atggtgccaa
    80521 tgcgctgggt actgtcggca atatcgtcca ttgtcttgat caccgtagtc atcgcctccc
    80581 cgccctgcac tgccgcatta ctggcggtga tagaaagttt atcagcagcc gacgccgtgg
    80641 cagagttctg tttcaccgac gccgccatct ggttcatggt cgccaccgtt tgctgaacgt
    80701 tatcaactgt ctgctgggta tgttcgttca gttcatcggt gcctttcgcc agcgtctcac
    80761 tgccatttct gacactggac acctggcttg agacatcgtt aattagccaa cggcacatca
    80821 ggccaagctg ccctaccgcg cgtaatgtca gccccagctc atcgctgcga ttcagatgct
    80881 caacactatt acgctctccg gtcgccacct tcagtgcctg acgggcgaca ttttctatcg
    80941 ggcggacaat ctgccattca aaacaggcgc ttgccaacaa taccactaac gcacagagga
    81001 tatacgtcac caccggggca gcaacaaacc aaagcatgac cgccagcaag ataaacatca
    81061 gggtcatcac gccacgcgcc cgccagcgaa gcggtaatga aggcagtttt cccagccagc
    81121 ctttacgcac caccaggcct ttatgaatac gcttactggt acgtccggcg ttcaacgctt
    81181 tgtacagcgg ctccaccgcc gcgatctctt catccgtcgc ccgggtacga atcgacatat
    81241 agccactgat ttttccctcg cgcaccatcg gtaccgcatt ggcccgcacc caataatggt
    81301 cgccattttt gcggcgattt ttcacgatgc cgctccaggg ctcccctttt ttcagggtga
    81361 accacatatc tgcaaacgcc gcttttggca tatccgggtg acgcaccatg ttgtgcggct
    81421 gcccttgtaa ctcttgcaag gtaaagccgc tcacctgcac aaaagtgtca ttagcatgag
    81481 tgatatagct ttgcagatcg gtagtggaca tcagagtggt atcgtccgcc agcggggtat
    81541 tttgctgggt gacatacgga tgagaagaca tgttcgcgtc ctgtgcaggt tatatggttg
    81601 ttaacttctt gtcagagttt atgtcggccc cactgcggtt atctttaact gattaatttg
    81661 atttagatcg caatttgcga ttaaagcata aatctaatcc cttgatttaa aatactttca
    81721 ctctgttact atacgaaaac gttaattatc ttgcccaaaa atcaggcagt tgttgccctg
    81781 aaagtgtgca tttgcgcagc aatcgccaaa tccatacccg acaaaaaccg tgcaaaacaa
    81841 caacagatgt taacaaatag cattaaatat tgcacaaatg ataaccacat ttgtgtttat
    81901 cccgattttc gcgatcgcag ccggagtggc gcaatccctg caatacttaa atcggtatca
    81961 tgtgatacgc gagcctccgg agcatatttt gaacaggtta ccttcgagcg catcggcttt
    82021 agcgtgcagc gcccacgccc tgaatctcat tgaaaagcga acgttggatc atgaggagat
    82081 gaaagcactt aaccgggagg tgattgaata cttcaaagag catgtcaatc cggggttttt
    82141 agagtatcga aaatctgtta ccgccggcgg ggattacgga gccgtagagt ggcaagcggg
    82201 aggtttaaat acgcttgtcg acacccaggg acaggagttt atcgactgcc ttggaggttt
    82261 tggaattttc aacgtggggc accgtaatcc agttgtggtt tccgccgtac agaatcaact
    82321 tgcgaaacaa ccgcttcaca gccaggagct gctcgatccg ttacgggcga tgctggcgaa
    82381 aacccttgcg gcgctaacgc ccggtaaact gaaatacagc ttcttctgta atagcggcac
    82441 cgagtccgtt gaagcagcgc tgaagctggc gaaggcttat cagtcaccgc gcggcaagtt
    82501 tacttttatt gccaccagcg gcgcgttcca cggtaaatca cttggcgcgc tgtcggccac
    82561 ggcgaaatcg accttccgca aaccatttat gccgctgctg ccagggttcc gtcatgtacc
    82621 gtttggcaat atcgaagcca tgcgcacggc ccttagcgag tgcaaaaaaa ccggtgatga
    82681 tgtggctgca gtgatcctcg aaccgattca gggcgaaggt ggcgtcattc tgccgccgcc
    82741 aggctatctc accgccgtgc gtaagctgtg cgatgagttc ggcgcactga tgatcctcga
    82801 tgaagtacaa acgggcatgg ggcgcacggg caagatgttc gcctgcgagc atgagaacgt
    82861 acagccggac atcctgtgtc tggcaaaagc cctcggcggc ggcgtgatgc cgattggcgc
    82921 aaccatcgcc actgaagagg tgttttccgt tctgttcgac aacccgttcc tgcataccac
    82981 cacctttggc ggcaacccgc tggcctgtgc ggcggcgctg gcgaccatca atgtgttgct
    83041 ggagcagaac ttaccggctc aggctgaaca aaaaggcgat atgttgctgg acggttttcg
    83101 tcaactggcg cgggaatatc ccgatctggt acaggaagcg cgtggtaaag ggatgttgat
    83161 ggcgattgag tttgttgata acgaaatcgg ctataacttt gccagcgaga tgttccgcca
    83221 gcgcgtcctg gtggccggaa cactcaacaa cgctaaaacg atccgcattg aaccgccact
    83281 gacactgacc attgaacagt gtgaacaggt gatcaaagcg gcgcgtaagg cgctggcggc
    83341 catacgagtc agtgtcgaag aagcgtaata tcaaatcgga tggcgattcg acgtcgccat
    83401 ccgatctttt tatacgatac gtacgcccgc aggcatcatc cgctccggcg ttaacagcac
    83461 gctttcactg ccgtcatcgg tttccgcaca caacagcatg cattccgacg tttcaccgcg
    83521 cattttcgct ttttgcagat tgcataagac caccactgtt tttcccatca gctcttcttc
    83581 gctgtagtac ggcaccaggc tggtcacggt ttgcagcgtt ttttcaccca catcaacctg
    83641 tacgatgtac agcttgtcgg cgttttcatg gcgtttcact tccacaatct ttccgacgcg
    83701 catttccaga cgtgcaaaat cagcgtaagc cacggtttcc attgtttcct cccttcgggt
    83761 aaaattttac taaactatag caaaaatttt tctcaatcct gtaggctaaa aatggagaat
    83821 gcaggcgtga tcacattcgc aagccgctgt gttaccgtta cagcgtcaaa gaaacgcgct
    83881 ttatttactg aaaacaggtg acccgataag cacttcctct acaatggggc gcacatcagg
    83941 gaaagtaaaa aaggtaaaca tggcaacact aaaagacatc gcaatcgaag ctggcgtatc
    84001 cctggcgaca gtatccaggg tcttaaatga cgatccgaca ttgaatgtga aagaagagac
    84061 gaaacatcgc attctcgaga tcgccgaaaa gctggagtac aagaccagta gtgcccgtaa
    84121 actccagaca ggtgcagtca accaacacca tattctggct atctacagct accaacagga
    84181 gctggagatc aacgatcctt actatctggc gatccgccac ggcattgaaa cccagtgcga
    84241 aaaactggcg atcgaactga ccaactgtta tgaacacagc ggcttaccag acattaaaaa
    84301 cgtcaccggt attttaattg tcggcaaacc cacgcccgcc ctgcgcgccg ccgcttgtgc
    84361 gttgaccgac aatatctgct ttatcgactt tcacgaaccc ggcagcggtt acgatgcggt
    84421 ggatatcgat ctggcacgta tcagtaaaga aatcatcgat ttctatatca atcagggcgt
    84481 taatcgtatt ggttttattg gcggtgaaga cttgcctggc aaggcggata ttcgtgaggt
    84541 cgcctttgtg gaatatggtc gtctgaaaca cgtggtacgc gaagaggata tctggcgcgg
    84601 cggtttttcc agttcgtcgg gctatgaact ggcaaaacag atgctggcgc aggaagacta
    84661 cccgaaggcg ttgtttgtcg cttctgattc cattgctatc ggcgtactgc gggcaattca
    84721 tgaacgaggc ctgaacatcc cacaggatat ttcgcttatc agcgtgaacg acatccccac
    84781 cgcgcgattt acctttccgc cgctctcaac cgtgcgcatc cattccgaga tgatgggaag
    84841 tcagggcgtt aacctgttgt atgaaaaggc tcgcgatggt cgcgcgctgc cgctgttagt
    84901 cttcgttccc agcaaattaa aactccgcgg cacgacccgc taaatcccct tacacactgt
    84961 ccggcaatcg tttttgccgg acagtgctgc cgtttatttt cgtgatccag ttaaagtaaa
    85021 tgcatttacc tgctactttt tagtaaaaat tttactaaac tccccagcaa ttacacaaac
    85081 taccatcacc atgaatggtt ccgatttctc tctaccggga ggccctatga atcgctggga
    85141 aaacattcag ctcacccacg aaaaccgact tgcgccgcgt gcgtactttt tttcatatga
    85201 ttctgttgcg caggcgcgta cttttgcccg tgaaaccagc agcctgtttc tgtccttaag
    85261 cggtcagtgg aatttccact tttttgacca tccgctgcaa gtaccagaag ccttcacctc
    85321 tgagttaatg gctgactggg ggcatattac cgtccccgcc atgtggcaaa tggaaggtca
    85381 cggcaaactg caatataccg acgaaggttt tccattcccc atcgatgtgc cgtttgttcc
    85441 cagcgataac ccaaccggtg cctatcaacg tattttcacc ctcagcgaag gctggcaggg
    85501 taaacagacg ctgatcaaat ttgacggtgt cgaaacctat tttgaagtct acgttaacgg
    85561 tcagtatgtg gggttcagca agggcagtcg cctgaccgca gagtttgaca tcagcgcgat
    85621 ggttaaaacc ggcgacaacc tgttgtgtgt gcgcgtgatg caatgggcgg actctaccta
    85681 cgtggaagac caggatatgt ggtggtcggc ggggattttc cgcgatgttt atctgatcgg
    85741 aaaacaactc acgcatatta acgatttcac cgttcgtacc gactttgacg aagcctattg
    85801 cgatgccacg ctttcctgcg aagtggtgct ggaaaatctc gccgcctccc ctgtcgtcac
    85861 gacgctggaa tataccctgt ttgatgggga acgcctggtg cacagcagcg ccattgatca
    85921 tttggcaatt gaaaaactga ccagcgccag ctttgctttt actgtcgaac agccccagca
    85981 atggtcagca gaatcccctt atctttacca tctggtcatg acgctgaaag acgccgacgg
    86041 caacgttctg gaagtggtgc cacaacgcgt tggcttccgt gatatcaaag tgcgcgacgg
    86101 tctgttctgg atcaataacc gttatgtgat gctgcatggc gtcaaccgcc acgacaatga
    86161 tcatcgcaaa ggccgcgccg ttgggatgga tcgcgtcgag aaagatctcc agttgatgaa
    86221 gcagcacaac atcaattccg tgcgtaccgc tcactacccg aacgatcctc gtttttacga
    86281 actgtgtgat atctacggcc tgtttgtgat ggcggaaacc gacgtcgaat cgcacggctt
    86341 tgctaacgtc ggcgatatca gccgcattac cgacgatccg cagtgggaaa acgtctacgt
    86401 cgagcgcatt gttcgccata ttcacgcgca gaaaaaccat ccgtcgatca tcatctggtc
    86461 gctgggcaat gaatccggct atggctgtaa catccgcgcg atgtaccatg cggcgaaggc
    86521 gctggatgac acgcgactgg tgcattacga agaagatcgc gatgctgaag tggtcgatat
    86581 tatttccacc atgtacaccc gcgtgccgct gatgaatgag tttggtgaat acccgcatcc
    86641 gaagccgcgc atcatctgtg aatatgctca tgcgatgggg aacggaccag gtgggctgac
    86701 ggagtaccag aacgtcttct acaagcacga ttgcattcag ggacattatg tctgggaatg
    86761 gtgcgaccac gggatccagg cgcaggatga caatggcaac gtctggtata aattcggcgg
    86821 cgactacggc gactatccca acaactataa cttctgtctt gatggtttga tctattccga
    86881 tcagacgccg ggaccaggcc tgaaagagta caaacaggtt attgcgccgg taaaaatcca
    86941 cgcgctggat ctgactcgcg gcgagttgaa agtcgaaaat aaactatggt ttaccacgct
    87001 tgatgactac accctgcacg cagaggtgcg cgccgaaggt gaaacactcg cgacgcagca
    87061 gattaaactg cgcgacgttg cgccgaacag cgaagccccc ttgcagatca cgctgccgca
    87121 gctggacgcc cgcgaagcgt tcctcaacat tacggtgacc aaagattccc gcacccgcta
    87181 cagcgaagcc gggcattcta tcgccactta tcagttcccg ctgaaggaaa acaccgcgca
    87241 gccagtgcct ttcgcaccaa ataatgcgcg tccgctgacg ctggaagacg atcgtttgag
    87301 ctgcaccgtt cgcggctaca acttcgcgat caccttctca aaaatgagtg gcaaaccgac
    87361 atcctggcag gtgaatggcg agtcgctgct gacccgcgag ccaaagatca acttcttcaa
    87421 gccaatgatc gacaaccaca agcaggagta cgaaggactg tggcaaccga atcatttgca
    87481 gatcatgcag gaacatctgc gcgactttgc cgttgagcaa agtgatgatg aagtgttgat
    87541 catcagccgc acggttattg ccccaccggt gtttgacttc gggatgcgct gtacctacat
    87601 ctggcgcatc gctgccgatg gtcaggttaa tgtggcgctt tccggcgaac gttacggcga
    87661 ctatccgcac atcattccgt gcatcggttt caccatgggg attaacggcg aatacgacca
    87721 ggtagcatat tacggacgtg gaccgggcga aaactacgcc gacagccagc aggctaacat
    87781 catcgatatc tggcgcagca ccgtcgatgc catgttcgag aactatccct tcccgcagaa
    87841 caacggcaac cgtcagcatg tccgctggac ggcactgact aaccgccacg gcaacggtct
    87901 gctggtggtt ccgcagcgcc ccattaactt cagcgcctgg cgctataccc aggaaaacat
    87961 ccacgctgcc cagcactgta acgagcttca gcgcagtgat gacatcactc tgaatctcga
    88021 ccaccagctg cttggcctcg gctccaactc ctggggcagc gaggtgctgg actcctggcg
    88081 cgtctggttc cgtgacttca gctacggctt tacgttgctg ccggtttctg gcggagaagc
    88141 taccgcgcaa agcctggcgt cgtatgagtt cggcgcaggg ttcttttcca cgaatttgca
    88201 cagcgagaat aagcaatgag gatcatcgat aacttagaac agttccgcca gatttacgcc
    88261 tctggcaaga agtggcaacg atgcgttgaa gcgattgaaa atatcgacaa cattcagcct
    88321 ggcgtcgccc actccatcgg tgactcattg acttaccgcg tggagacgga ctccgcgacc
    88381 gatgcgctat ttaccgggca tcgacgctat tttgaagtgc attactacct gcaagggcag
    88441 caaaaaattg aatatgcgcc gaaagagaca ttacaggttg tggaatatta tcgtgatgaa
    88501 actgaccgtg aatatttaaa aggctgcgga gaaaccgtag aggtccacga agggcaaatc
    88561 gttatttgcg ataaccatga agcatatcgg tttatctgca ataacgcggt caaaaaagtg
    88621 gttctcaaag tcaccatcga agatggttat ttccataaca aataacaact acggcggcaa
    88681 aaggagtttg ccgccaccgc taccctactc attttcggag atgtgttatg tctgatacca
    88741 aacgtaatac aatcggcaaa ttcggcttgc tctcgctgac ttttgccgcc gtttacagct
    88801 ttaacaacgt tatcaataat aatattgagc ttggactggc ctcggcaccg atgtttttcc
    88861 tcgcgacgat tttttatttt attcccttct gtctgatcat tgcagaattt gtttcgttaa
    88921 ataaaaactc agaagccggt gtctacgcgt gggtaaaaag ttcgctgggc ggacgttggg
    88981 catttattac tgcctatacc tactggttcg taaacctgtt ctttttcacc tcgctgttac
    89041 cgcgcgttat tgcttatgct tcgtatgcct tcctcggcta cgaatatatt atgacgccgg
    89101 ttgccaccac cattatcagt atggtgctgt tcgccttctc cacatgggtt tccaccaacg
    89161 gggcgaaaat gctggggcca atcacctccg tcacttcaac gctgatgctg ctgttaacgc
    89221 tctcctacat tttactggca ggtacggcac tggttggcgg cgtacagcct gccgatccga
    89281 tcaccgttga cgcgatgatc ccgaacttca actgggcgtt cctcggcgtg accacctgga
    89341 tctttatggc cgcaggtggc gcggagtccg tcgcggtgta cgttaacgac gtcaaaggcg
    89401 gttcgaaatc gttcgttaaa gtgatcatcc tcgccgggat ttttatcggc gtactgtatt
    89461 ccgtctcctc ggtgctgatt aacgtcttcg tcagcagcaa agagttgaaa tttactggcg
    89521 gatcggtaca ggtattccac ggcatggcgg cgtattttgg tctaccggaa gcactgatga
    89581 atcgctttgt cggtctggtg tcctttaccg cgatgttcgg ttccctgctg atgtggacgg
    89641 caacgccggt gaaaattttc ttctccgaaa ttccggaagg catctttggt aagaaaaccg
    89701 tcgaactgaa cgaaaacggc gttccggcgc gcgcagcgtg gatccagttc ctgatcgtca
    89761 tcccgctgat gattatcccg atgctcggtt ccaataccgt gcaggatctg atgaatacca
    89821 ttattaatat gaccgccgca gcgtccatgc ttccgccgtt attcatcatg ctggcttacc
    89881 tgaatttacg cgccaaatta gatcacctgc cacgcgattt ccgtatgggt tcccgacgca
    89941 ccggtattat cgttgtttca atgctgattg cgatatttgc tgtagggttt gtcgcttcga
    90001 cattcccgac tggcgcgaat attctgacca tcatttttta taacgtcggc ggtattgtta
    90061 tcttcctccg ctttgcgtgg tggaaataca gtaaatatat aaagggatta acggctgaag
    90121 agcgccatat tgaagcgacg ccagccagca atgttgatta agcagaataa taaaaaatga
    90181 gcaggcattt tccctcttcc tggatgggga gagggaaaat agtttctgcc tttatatttt
    90241 tataattaca acgataaaag gctgtacttt ttctttagct catggattaa cacaatgaaa
    90301 ttaatcactg caccatgcag agcattactt gctctgccgt tttgctacgc cttttctgcg
    90361 gccgctgaag aagcacgtcc ggcagaacat gacgacacaa aaacacccgc aattacctcg
    90421 acatcttctc cttcatttcg tttttacggc gaattagggg ttggtgggta tatggattta
    90481 gagggtgaga ataaacataa atacagcgac ggcacctata ttgaaggtgg cctggagatg
    90541 aagtacggct cctggttcgg cctgatttac ggcgaaggct ggaccgtgca ggccgaccac
    90601 gacggtaatg cctgggtgcc tgaccatagc tggggcggtt tcgaaggtgg gattaaccgt
    90661 ttctatggcg gttatcgtac caatgatggc accgaaatca tgctcagtct gcgtcaggat
    90721 tcctcgctgg atgatctgca atggtggggc gacttcaccc ccgatctggg ctacgtcatt
    90781 cccaataccc gcgacattat gaccgcgctg aaggtgcaga acttaaccgg caactttcgt
    90841 tatagcgtca ccgccacacc agccggacat catgacgaaa gcaaagcctg gctacatttt
    90901 ggcaaatacg atcgctacga cgacaaatac acttatccgg caatgatgaa cggttacatc
    90961 cagtatgacc ttgcagaagg tatcacctgg atgaacggtc tggaaatcac cgacggcaca
    91021 ggacaactct atctgacggg cctgcttacc cctaactttg ccgctcgcgc ctggcaccat
    91081 accggacgcg ccgacgggct ggacgtaccg ggaagtgaaa gcgggatgat ggtgagcgcc
    91141 atgtatgaag cgttaaaagg cgtttatctc tccaccgctt acacctacgc caaacatcgc
    91201 cctgaccacg ctgacgatga aaccacctct ttcatgcagt ttggtatctg gtacgaatac
    91261 ggcggcggac gtttcgcaac ggcttttgat agccgcttct acatgaaaaa tgcctctcac
    91321 gatcccagcg accaactctt cctgatgcaa tatttctact ggtaataagg actgtaattt
    91381 catgaaaatc aaaactattt taacgccagt aacctgcgct ctgctgataa gtttttccgc
    91441 ccatgccgct aacgccgaca attataaaaa cgtgattaac cgtactggcg cgccgcagta
    91501 catgaaggat tacgattacg acgatcacca gcgttttaat ccgtttttcg atctcggagc
    91561 ctggcatggt catctgttgc cagacggccc gaacaccatg ggaggttttc ctggcgttgc
    91621 gctgctgacg gaagaataca tcaactttat ggccagcaat ttcgatcgcc tgaccgtctg
    91681 gcaagacggc aagaaagtcg acttcacgct ggaggcatac agtattcccg gcgcgctggt
    91741 gcaaaaactg acagcaaaag atgtgcaggt cgaaatgact ctgcgcttcg ccacgccgcg
    91801 cacatcacta ctggaaacca aaatcaccag cgataaaccg ctggatctgg tgtgggatgg
    91861 tgaactgctg gaaaaactgg aagcgaaaga agggaaaccg ctttccgata aaaccattgc
    91921 tggcgaatac cctgactatc agcgcaaaat cagcgccacc cgtgatggcc tgaaagtcac
    91981 ctttggcaaa gtgcgcgcca cctgggatct gctgacctcc ggtgaatcag aatatcaggt
    92041 gcataaatcc ctgccagtgc agactgaaat caacggcaat cgctttacca gtaaggcgca
    92101 tatcaacggt tcgaccacgc tctataccac ctattcccat ctgctgaccg ctcaggaagt
    92161 tagcaaagag caaatgcaga tccgcgatat tctggcgcgt ccggcgtttt atctcaccgc
    92221 ctcgcagcaa cgctgggaag agtatctgaa gaaagggtta accaatccgg atgcgacgcc
    92281 ggaacagacg cgcgtcgcgg tgaaagccat cgaaacactc aacggtaact ggcgctcacc
    92341 gggcggggcg gtgaaattta ataccgtcac accgtcggtg accggacgct ggttctccgg
    92401 caatcagacc tggccgtggg atacctggaa acaggcgttt gcgatggcgc atttcaatcc
    92461 ggacatcgcc aaagagaata tccgcgcggt cttctcctgg cagatccaac ctggcgacac
    92521 cgtgcgtcca caggatgtgg gatttgtctc cgacctgatt gcgtggaacc ttagccccga
    92581 gcgtggcggc gatggcggca actggaacga acgcaatacc aagcccagcc ttgccgcctg
    92641 gtcggtgatg gaagtgtaca acgtcaccca ggataaaacc tggctggcag agatgtaccc
    92701 gaaactggtg gcctatcacg actggtggtt acgtaaccgc gatcataacg gcaacggcgt
    92761 gccggaatat ggcgcgaccc gcgataaagc ccataacacc aagagcggcg agatgctgtt
    92821 tacggtgaaa aaaggcgata aagaagagac gctgtctggg ctgaacaact acgcccgcgt
    92881 ggtggagaaa ggccagtacg acagtctgga aatcccggca caagttgctg cgtcgtggga
    92941 atcaggtcgt gatgacgccg ccgtctttgg gtttatcgac aaagaacagc tggataaata
    93001 tgtcgctaac ggcggcaaac gtagcgactg gacggtgaaa ttcgccgaaa accgcagtca
    93061 ggacggaacg ttgctgggct actcgctatt gcaggagtcg gtggatcagg ccagctatat
    93121 gtacagcgat aaccattatc tggcggagat ggcaaccata ctcggtaagc cggaagaggc
    93181 caaacgctat cgccagttgg cacagcagct cgcggactac atcaacacct gtatgttcga
    93241 cccgactaca cagttctact atgacgtgcg cattgaagat aaaccactgg cgaacggctg
    93301 cgcgggcaaa ccgattgttg agcgcggtaa agggccagaa ggctggtcgc cgctgtttaa
    93361 cggtgcggca acgcaggcca acgccgacgc ggtggtgaag gtgatgctcg atcctaaaga
    93421 gttcaacacc tttgtaccgc tgggaacggc agcgttaacc aacccggctt ttggcgctga
    93481 tatctactgg cgcgggcgcg tatgggtaga tcagttctgg tttggtctga aaggaatgga
    93541 gcgttacggt tatcgtgatg atgccctgaa actggcggat acgttcttcc agcatgccaa
    93601 aggattaacc gccgatggcc cgattcagga gaattacaac ccgctgactg gcgcacagca
    93661 aggcgcacca aatttctcct ggagtgccgc gcatttgtat atgttgtata acgatttttt
    93721 ccgtaagcag taatttttcc cgatgccgga cgactgtatt accgccgtcc ggcatgacga
    93781 acgggcgtat tctttttgaa tcccatcaca aaccccgcac tccccttttc ccttttctcc
    93841 ggcgacggct aaattagaac tcatccgacc acataacaat tattttacat actggacaat
    93901 tttatgagct acccgtcgct gttcgcctcg ctggatttag gttttaccac gttaaaaaac
    93961 cgcgtgttga tgggttcaat gcacaccggg ctggaggaat acccggacgg tgctgaacgg
    94021 ctggcagcgt tttatgccga acgcgcccgt catggcgtgg cgttgattgt cagcggcggc
    94081 attgcaccag atttaacagg cgttggcatg gaaggcggcg caatgctcaa tgacgccagc
    94141 cagatcccac accatcgcac cattaccgaa gccgtacatc aggaaggcgg caaaatcgcc
    94201 ctgcaaattt tgcataccgg gcgctacagc taccaaccgc atctggtcgc cccctccgca
    94261 ttgcaggccc ccatcaaccg tttcgtgccc catgagttaa gccatgaaga gatcctgcaa
    94321 ctgatcgaca atttcgcccg ctgcgcgcaa ctggcacggg aggcaggata cgacggtgta
    94381 gaggtgatgg gttccgaagg gtatttgatc aacgaatttc tgacgctgcg caccaatcag
    94441 cgtagtgacc agtggggcgg cgattaccac aaccggatgc ggtttgcggt tgaagtggtg
    94501 agtgcggtac gcgaacgcgt tggcaacgac ttcattatta tctaccgttt gtcgatgctc
    94561 gatctggtcg aagacggcgg gacttttgcc gaaacggtag agctggcgca ggccattgaa
    94621 gcggcgggcg cgaccattat caacaccggc attggctggc atgaagcgcg gattccgacc
    94681 atcgccacgc ccgtgccgcg cggcgcattt agctgggtca cacgcaaact gaaaggccac
    94741 gtttcgctgc cgctggtgac caccaaccgg attaacgatc cgcaggttgc cgacgatatt
    94801 ctcgcgcgcg gtgatgccga tatggtatcg atggcgcgac cgtttcttgc tgatgcggag
    94861 ctgctgtcaa aagcgcaatc agggcgagcc gatgagatca acacctgcat tggctgcaat
    94921 caagcctgcc tcgatcagat cttcgttggc aaagtcacct cgtgcctggt gaatcctcgc
    94981 gcctgccacg aaaccaaaat gccgatcctt cccgccgtgc agaaaaaaaa tctggcggtg
    95041 gtcggcgcag gtccggcggg gctggcgttt gccatcaatg cggcggcgcg tggtcatcag
    95101 gtaacgttgt ttgatgcaca tagcgagatc ggcgggcagt ttaatatcgc caaacagatc
    95161 cccggcaaag aggaatttta cgaaacattg cgctactacc gccggatgat cgaagtgact
    95221 ggcgtgacac tgaaacttaa tcacaccgtg accgcagatc agttaaaggc gttcgatgaa
    95281 acgatcctcg ccagtgggat cgtaccgcgc attccgccca tcgacgggat cgatcatccg
    95341 aaggtgttga gttatctcga cgtactgcgc gacaaagcgc cggtaggcaa caaagttgcc
    95401 atcatcggtt gtggcgggat tggttttgat acggcgatgt atttaagtca gccgggcgaa
    95461 tccaccagcc agaatatcgc cgggttctgt aatgaatggg ggatcgacag cagcctgcaa
    95521 caggctggtg gcttaagccc gcagggaatg cagatcccgc gtagcccacg gcagattgtg
    95581 atgctccagc gcaaagccag caaaccggga caggggttag gcaaaaccac cggctggatc
    95641 catagcacca ccctgctctc gcgcggtgtg aaaatgatcc caggcgtaag ttatcagaag
    95701 attgacgatg acgggctgca tgtggtgatc aacggcgaaa cgcaggtatt agcagtggac
    95761 aatgtggtga tctgcgcagg gcaagagcca aaccgcgcat tggcgcaacc gctgattgat
    95821 agcgggaaaa ctgtgcattt aattggcggc tgcgatgtag ctatggagct ggacgcacga
    95881 cgggcaattg cccagggaac acggctggcg ctggagattt aaattgtgca ttgaagtgcc
    95941 ggatgcgacg cttgccgcgt cttatccgac ctgggggtaa tgtgctgaac gtatgccaga
    96001 taaggcgttt acgccttatc tggcaggtag ctgccaatct taccgacgac gccccagctt
    96061 cactgctttc agcaccacaa atttattat
//
LOCUS       DAMZFT010000004        94884 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.004, whole genome shotgun sequence.
ACCESSION   DAMZFT010000004 DAMZFT010000000
VERSION     DAMZFT010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 94884)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 94884)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..94884
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.004"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..1000
                     /gene="rtcR"
                     /locus_tag="PN977_000302"
     CDS             <1..1000
                     /gene="rtcR"
                     /locus_tag="PN977_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RtcR"
                     /protein_id="HDI6040384.1"
                     /translation="EKVAIKSRAPILLNGPTGAGKSFLARRIFELKQARHQFSGAFVE
                     VNCATLRGDTAMSALFGHVKGAFTGARESREGLLRSANGGMLFLDEIGELGADEQAML
                     LKAIEEKTFYPFGSDRQVSSDFQLIAGTVRDLRQLVAEGKFREDLYVRINLWTFTLPG
                     LRQRQEDIEPNLDYEVERHASLTGDSVRFNTEARRAWLAFATSSQAAWRGNFRELSAS
                     VTRMATFATSGRITLEVVGDEINRLRYNWQESRPSALTALLGAEAENIDLFDRMQLEH
                     VIAICRQAKSLSAAGRQLFDVSRQGKASVNDADRLRKYLARFNLTWEAVQDQHSSS"
     gene            complement(982..1740)
                     /locus_tag="PN977_000303"
     CDS             complement(982..1740)
                     /locus_tag="PN977_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000815090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family transcriptional regulator"
                     /protein_id="HDI6040385.1"
                     /translation="MKQTQRHNGIIELVKQQGYVSTEELVEHFSVSPQTIRRDLNELA
                     EQNLILRHHGGAALPSSSVNTPWHDRKATQTEEKERIARKVAEQIPNGSTLFIDIGTT
                     PEAVAHALLNHSNLRIVTNNLNVANTLMVKEDFRIILAGGELRSRDGGIIGEATLDFI
                     SQFRLDFGILGISGIDSDGSLLEFDYHEVRTKRAIIENSRHVMLVVDHSKFGRNAMVN
                     MGSISMVDAVYTDAPPPVSVMQVLTDHHIQLELC"
     gene            complement(1757..2587)
                     /gene="glpG"
                     /locus_tag="PN977_000304"
     CDS             complement(1757..2587)
                     /gene="glpG"
                     /locus_tag="PN977_000304"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease
                     GlpG"
                     /protein_id="HDI6040386.1"
                     /translation="MLMITSFANPRVAQAFVDYMATQGVILTIQQHNQSDVWLADESQ
                     AERVRAELARFLENPADPRYLAASWQAGHTGSGLHYRRYPFFAALRERAGPVTWVMMI
                     ACVVVFIAMQILGDQEVMLWLAWPFDPTLKFEFWRYFTHALMHFSLMHILFNLLWWWY
                     LGGAVEKRLGSGKLIVITLISALLSGYVQQKFSGPWFGGLSGVVYALMGYVWLRGERD
                     PQSGIYLQRGLIIFALIWIVAGWFDLFGMSMANGAHIAGLAVGLAMAFVDSLNARKRK
                     "
     gene            complement(2632..2958)
                     /gene="glpE"
                     /locus_tag="PN977_000305"
     CDS             complement(2632..2958)
                     /gene="glpE"
                     /locus_tag="PN977_000305"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase GlpE"
                     /protein_id="HDI6040387.1"
                     /translation="MDQFECINVADAHQKLQEKEAVLVDIRDPQSFAMGHAAQAFHLT
                     NDTLGAFMRDNDFDTPVMVMCYHGNSSKGAAQYLLQQGYDVVYSIDGGFEAWQRQFPA
                     EVAYGA"
     gene            3148..4653
                     /gene="glpD"
                     /locus_tag="PN977_000306"
     CDS             3148..4653
                     /gene="glpD"
                     /locus_tag="PN977_000306"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase"
                     /protein_id="HDI6040388.1"
                     /translation="METKDLIVIGGGINGAGIAADAAGRGLSVLMLEAQDLACATSSA
                     SSKLIHGGLRYLEHYEFRLVSEALAEREVLLKMAPHIAFPMRFRLPHRPHLRPAWMIR
                     IGLFMYDHLGKRTSLPGSTGLRFGANSVLKPEIKRGFEYSDCWVDDARLVLANAQMVV
                     RKGGEVLTRTRATSARRENGLWIVEAEDIDTGKKYTWQARGLVNATGPWVKQFFDDGM
                     HLPSPYGIRLIKGSHIVVPRVHTQKQAYILQNEDKRIVFVIPWMDEFSIIGTTDVEYK
                     GDPKAVKIEESEINYLLKVYNTHFKKQLSRDDIVWTYSGVRPLCDDESDSPQAITRDY
                     TLDIHDENGKAPLLSVFGGKLTTYRKLAEHALEKLTPYYQGIGPAWTKESVLPGGAIE
                     GDRDDYAARLRRRYPFLTESLARHYARTYGSNSELLLGNAGAISDLGEDFGHEFYEAE
                     LKYLVDHEWVRRADDALWRRTKQGMWLNADQQSRVSQWLVEYTQQKLSLAS"
     gene            complement(4868..5468)
                     /locus_tag="PN977_000307"
                     /pseudo
     CDS             complement(4868..5468)
                     /locus_tag="PN977_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016159550.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(5471..6463)
                     /locus_tag="PN977_000308"
     CDS             complement(5471..6463)
                     /locus_tag="PN977_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PstS family phosphate ABC transporter
                     substrate-binding protein"
                     /protein_id="HDI6040389.1"
                     /translation="MNKFTGVLLLGTALLAGCVDREGYYNSVREEDSHGLTSLRGQPE
                     LRYNDDWSRWPRVYGATALYPLYASAYYELVPEPKDKDRTSLAWQAYGLQQTRTAEAY
                     DSLIKGTATVIFVAQPSEGQKKRAEEAGVKLKYTAFAREAFVFIVDIKNPVNSLSEQQ
                     VKDIFSGKVSRWNKVGGGDESIKVWQRPEDSGSQTVMKGLVMQDTPMLPAKKSTVIDL
                     MGGLITEVADYQNTPSSIGYTFHYYVTRMNDNMLKMRKQIKLLAINGVAPTEENIRNG
                     TYPYIIHAYMVTHENPTLETQKFVDWFLSPQGQQLVEDVGYVPIYDASSESSGQ"
     gene            complement(6489..7997)
                     /locus_tag="PN977_000309"
     CDS             complement(6489..7997)
                     /locus_tag="PN977_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PstS family phosphate ABC transporter
                     substrate-binding protein"
                     /protein_id="HDI6040390.1"
                     /translation="MMQNGKWILTSLVMTFFGIPILAQFLAAVVAMLGAGLAAIFDVC
                     NLLFTPTIYLLLNVFMLTLGALLLFFSGRVWAGDSAPENREIAAWRQCLFLVPALLTL
                     VGWIITLHLADYQFRQMVSGWLANLMLPWLGVFTVSFVGGEYWWIVIIPVGAHISFSL
                     GYGWPTRHPLTGTSGLRCRNLLLFILLLLGIVAGYQAYLYKQLNPGVGVRENIDTWAW
                     RPDKLNNQLTPLRGKPQIQFTQNWPRLDGATAAYPIYASAFYALSVIPEDFHVWDYLD
                     NSRTQEAYNKIVKGDADIIFVAQPSDGQKKRAEKSGVTLLYTPFAREAFVFIVNADNP
                     VNSLTEQQVRDIFSGAITNWRTVGGNDQEIQTWQRPEDSGSQTVMQSQVMKNVRMISP
                     QETEVASMMEGMIKVVAEYRNTNNAIGYTFRYYATQMNADKNIKLLAINGIAPTAENI
                     RNGKYPYIIDAFMVTRENTTSETQKLVEWFLTPQGQSLVEDVGYVPLYPTMK"
     gene            complement(8123..10570)
                     /gene="glgP"
                     /locus_tag="PN977_000310"
     CDS             complement(8123..10570)
                     /gene="glgP"
                     /locus_tag="PN977_000310"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen phosphorylase"
                     /protein_id="HDI6040391.1"
                     /translation="MNAPFTYSSPTLSVEALKHSIAYKLMFTIGKDPVVANKHEWLNA
                     TLFAVRDRLVERWLRSNRAQLSQETRQVYYLSMEFLIGRTLSNAMLSLGIYEDVQGAL
                     EAMGLNLEELIDEENDPGLGNGGLGRLAACFLDSLATLGLPGRGYGIRYDYGMFKQNI
                     VNGSQKESPDYWLEYGNPWEFKRHNTRYKVRFGGRIQQEGKKTRWIETEEILGVAYDQ
                     IIPGYDTDATNTLRLWSAQASSEINLGKFNQGDYFAAVEDKNHSENVSRVLYPDDSTY
                     SGRELRLRQEYFLVSSTIQDILSRHYQLHKTYDNLADKIAIHLNDTHPVLSIPEMMRL
                     LIDEHQFSWDDAFEVCCQVFSYTNHTLMSEALETWPVDMLGKILPRHLQIIFEINDYF
                     LKTLQEQYPNDTDLLGRASIIDESNGRRVRMAWLAVVVSHKVNGVSELHSNLMVQSLF
                     ADFAKIFPGRFTNVTNGVTPRRWLAVANPSLSAVLDEHLGRNWRTDLSLLNELQQHCD
                     FPMVNHAVHQAKLENKKRLAEYIAQQLNVVVNPKALFDVQIKRIHEYKRQLMNVLHVI
                     TRYNRIKADPDAKWVPRVNIFGGKAASAYYMAKHIIHLINDVAKVINNDPQIGDKLKV
                     VFIPNYSVSLAQLIIPAADLSEQISLAGTEASGTSNMKFALNGALTIGTLDGANVEML
                     DHVGADNIFIFGNTAEEVEELRRQGYKPREYYEKDEELHQVLTQIGSGVFSPEDPGRY
                     RDLVDSLINFGDHYQVLADYRSYVDCQDKVDELYERQEEWTAKAMLNIANMGYFSSDR
                     TIKEYADHIWHIDPVRL"
     gene            complement(10589..12022)
                     /gene="glgA"
                     /locus_tag="PN977_000311"
     CDS             complement(10589..12022)
                     /gene="glgA"
                     /locus_tag="PN977_000311"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HDI6040392.1"
                     /translation="MQVLHVCSEMFPLLKTGGLADVIGALPAAQIADGVDARVLLPAF
                     PDIRRGVTDAQVVSRRDTFAGHITLLFGHYNGVGIYLIDAPHLYDRPGSPYHDTNLFA
                     YTDNVLRFALLGWVGAEMASGLDPFWRPDVVHAHDWHAGLAPAYLAARGRPAKSVFTV
                     HNLAYQGMFYAHHMNDIQLPWSFFNIHGLEFNGQISFLKAGLYYADHITAVSPTYARE
                     ITEPQFAYGMEGLLQQRHREGRLSGVLNGVDEKIWSPETDLLLASRYTRDTLEDKAEN
                     KRQLQIAMGLKVDDKVPLFAVVSRLTSQKGLDLVLEALPGLLEQGGQLALLGAGDPVL
                     QEGFLAAAAEYPGQVGVQIGYHEAFSHRIMGGADVILVPSRFEPCGLTQLYGLKYGTL
                     PLVRRTGGLADTVSDCSLENLADGVASGFVFEDSNAWSLLRAIRRAFVLWSRPSLWRF
                     VQRQAMAMDFSWQVAAKSYRELYYRLK"
     gene            complement(12022..13317)
                     /gene="glgC"
                     /locus_tag="PN977_000312"
     CDS             complement(12022..13317)
                     /gene="glgC"
                     /locus_tag="PN977_000312"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HDI6040393.1"
                     /translation="MVSLEKNDHLMLARQLPLKSVALILAGGRGTRLKDLTNKRAKPA
                     VHFGGKFRIIDFALSNCINSGIRRMGVITQYQSHTLVQHIQRGWSFFNEEMNEFVDLL
                     PAQQRMKGENWYRGTADAVTQNLDIIRRYKAEYVVILAGDHIYKQDYSRMLIDHVEKG
                     ARCTVACMPVPIEEASAFGVMAVDENDKIIEFVEKPANPPSMPNDPSKSLASMGIYVF
                     DADYLYELLEEDDRDENSSHDFGKDLIPKITEAGLAYAHPFPLSCVQSDPDAEPYWRD
                     VGTLEAYWKANLDLASVVPELDMYDRNWPIRTYNESLPPAKFVQDRSGSHGMTLNSLV
                     SGGCVISGSVVVQSVLFSRVRVNSFCNIDSAVLLPEVWVGRSCRLRRCVIDRACVIPE
                     GMVIGENAEEDARRFYRSEEGIVLVTREMLRKLGHKQER"
     gene            complement(13335..15308)
                     /gene="glgX"
                     /locus_tag="PN977_000313"
     CDS             complement(13335..15308)
                     /gene="glgX"
                     /locus_tag="PN977_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HDI6040394.1"
                     /translation="MTQLAIGKPTPLGAHYDGQGVNFTLFSAHAERVELCVFDANGQE
                     HRYDLPGHSGDIWHGYLPDARPGLRYGYRVHGPWQPAEGHRFNPAKLLIDPCARQIDG
                     EFKDNPLLHAGHNEPDYRDNAAIAPKCVVVVDHYDWEDDAPPRTPWGSTIIYEAHVKG
                     LTYLHPEIPVEIRGTYKALGHPVMINYLKQLGITALELLPVAQFASEPRLQRMGLSNY
                     WGYNPVAMFALHPAYACSPETALDEFRDAIKALHKAGIEVILDIVLNHSAELDLDGPL
                     FSLRGIDNRSYYWIREDGDYHNWTGCGNTLNLSHPAVVDYASACLRYWVETCHVDGFR
                     FDLAAVMGRTPEFRQDAPLFTAIQNCPVLSQVKLIAEPWDIAPGGYQVGNFPPLFAEW
                     NDHFRDAARRFWLHYDLPLGAFAGRFAASSDVFKRNDRLPSAAINLVTAHDGFTLRDC
                     VCFNHKHNEANGEENRDGTNNNYSNNHGKEGLGGTLDLVERRRDSIHALLTTLLLSQG
                     TPMLLAGDEHGHSQHGNNNAYCQDNQLTWLDWSQASSGLTAFTAALIHLRKRIPALVE
                     NRWWEEGDGNVRWLNRYAQPLSTDEWQNGPKQLQILLSDRFLIAINATLEVTEIVLPA
                     GEWHAIPPFAGEDNPVITAVWQGPAHGLCVFQR"
     gene            complement(15305..17491)
                     /gene="glgB"
                     /locus_tag="PN977_000314"
     CDS             complement(15305..17491)
                     /gene="glgB"
                     /locus_tag="PN977_000314"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching enzyme"
                     /protein_id="HDI6040395.1"
                     /translation="MSDRIDRDVINALIAGHFADPFSVLGMHKTTAGLEVRALLPDAT
                     DVWVIEPKTGRKLAKLECLDSRGFFSGVIPRRKNFFRYQLAVVWHGQQNLIDDPYRFG
                     PLIQEMDAWLLSEGTHLRPYETLGAHADTMDGVTGTRFSVWAPNARRVSVVGQFNYWD
                     GRRHPMRLRKESGIWELFIPGAHNGQLYKYEMIDANGNLRLKSDPYAFEAQMRPETAS
                     LICGLPKKVVQTEERKKANQFDAPISIYEVHLGSWRRHTDNNFWLSYRELADQLVPYA
                     KWMGFTHLELLPINEHPFDGSWGYQPTGLYAPTRRFGTRDDFRYFIDAAHAAGLNVIL
                     DWVPGHFPTDDFALAEFDGTNLYEHSDPREGYHQDWNTLIYNYGRREVSNFLVGNALY
                     WIERFGIDALRVDAVASMIYRDYSRKEGEWIPNEFGGRENLEAIEFLRNTNRILGEQV
                     SGAVTMAEESTDFPGVSRPQDMGGLGFWYKWNLGWMHDTLDYMKLDPVYRQYHHDKLT
                     FGMLYNYTENFVLPLSHDEVVHGKKSILDRMPGDAWQKFANLRAYYGWMWAFPGKKLL
                     FMGNEFAQGREWNHDASLDWHLLEGGDNWHHGVQRLVRDLNLTYRHHKAMHELDFDPY
                     GFEWLVVDDKERSVLIFVRRDKEGNEIIVASNFTPVPRHDYRFGINQPGKWREILNTD
                     SMHYHGSNAGNGGTVHSDEIASHGRQHSLSLTLPPLATIWLVREAE"
     gene            complement(17764..18867)
                     /gene="asd"
                     /locus_tag="PN977_000315"
     CDS             complement(17764..18867)
                     /gene="asd"
                     /locus_tag="PN977_000315"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HDI6040396.1"
                     /translation="MKNVGFIGWRGMVGSVLMQRMVEERDFDAIRPVFFSTSQLGQAA
                     PSFGGTTDTLQDAFDLEALKALDIIVTCQGGDYTNEIYPKLRESGWQGYWIDAASSLR
                     MKDDAIIILDPVNQDVITDGLNNGIRTFVGGNCTVSLMLMSLGGLFANDLVDWVSVAT
                     YQAASGGGARHMRELLTQMGHLYGHVADELATPSSAILDIERKVTTLTRSGELPVDNF
                     GVPLAGSLIPWIDKQLDNGQSREEWKGQAETNKILNTSSVIPVDGLCVRVGALRCHSQ
                     AFTIKLKKDVSIPTVEELLAAHNPWAKVVPNDREITMRELTPAAVTGTLTTPVGRLRK
                     LNMGPEFLSAFTVGDQLLWGAAEPLRRMLRQLA"
     gene            19059..19652
                     /gene="yhgN"
                     /locus_tag="PN977_000316"
     CDS             19059..19652
                     /gene="yhgN"
                     /locus_tag="PN977_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YhgN"
                     /protein_id="HDI6040397.1"
                     /translation="MNEIISAAVLLILIMDPLGNLPIFMSVLKHTEPKRRRAIMVREL
                     LIALLVMLVFLFAGEKILAFLSLRAETVSISGGIILFLIAIKMIFPSASGNSSGLPAG
                     EEPFIVPLAIPLVAGPTILATLMLLSHQYPNQMGHLVIALLLAWGGTFVILLQSSLFL
                     RLLGEKGVNALERLMGLILVMMATQMFLDGIRMWMKG"
     gene            complement(19698..20993)
                     /locus_tag="PN977_000317"
     CDS             complement(19698..20993)
                     /locus_tag="PN977_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312307.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HDI6040398.1"
                     /translation="MDADSSDSTVTPGRLPDGLSSCPCVYLGKKLMNLSANAQATLLL
                     TSDFSRAAASEYKPLSNSEWGKFALWLKHQRISPADLLVPQPQEKLTGWSDPRISQER
                     ILGLLARGHSLALAVDKWQRAGLWILTRGDADYPVRLKNRLRTDAPPVLFGCGNKALL
                     QAEGMAIVGSRDAPTADLRYTQQLAAKLAQQGICVISGGARGIDECAMASALEAGGTA
                     VGVLADSLLKTSTLVKWREGLIAGNLVLISPFYPEVRFTVGNAMARNKYIYCLAESAM
                     VVRAGMTGGTITGAMEALKHQWLPVQVKPNQDMQSANSRLVENGASWSTEQAENVTFR
                     LPDVTGLMYDKALRNAQPELFSLHEDDANYAVMPSHTPVDFYQLFVAELAILAKESIS
                     IERLASCTGLTIEQISVWLNRAEEEGRVIRLGEGHYQFR"
     gene            complement(20900..22996)
                     /locus_tag="PN977_000318"
     CDS             complement(20900..22996)
                     /locus_tag="PN977_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010349194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RecQ family ATP-dependent DNA helicase"
                     /protein_id="HDI6040399.1"
                     /translation="MEKHGAELLLQRMLGNTTATFREGQWEAIDAVVNQRRKLLVVQR
                     TGWGKSAVYFIASKIFRDRGAGTTIIISPLLALMRNQVAAAERLGITAETLNSTNREE
                     WQRISDKLLQGEVDCLLVSPERLANQDFIETVLYPIADRIGLLVVDEAHCISDWGHDF
                     RPDYRRILDILRQLPANTPVLGTTATANNRVVEDIRQQLGDIVIQRGTLARESLALDA
                     LVLGEQSSRLAWLATVIPQFSKSGIVYTLTTRDAELVAEWLRKNGISAFAYYSGVTCE
                     GAEDSNTAREYLEQALLANKIKVLVATTALGMGFDKPDLGFVIHYQMPGSIVGYYQQV
                     GRAGRAIDSAVGILLCGGEDRAIHQFFRESAFPAEAQIHEILNVLSENDGLTLRGIEQ
                     RTNLRYGQIEKALKLLVAENPSPVVYTEKLWRRTIVSFSPDHERINHLMNQRKSELAD
                     VESYITTKECKMQFLRRALDEPSAERCGKCSSCLQHPLLSPDIDSGLLHAANLFIKHA
                     DLPLNLNKQMAAGAFTQYGFKGNLPAGLQGSTGRILSRWGDSGWGKQVAQEKKTGRLS
                     DELVEACAEMVCQRWNPHPEPTWVCCVPSLRHLDLVPDFARRLAAKLGLPFIDAIEKV
                     VDNPPQKMQQNRFHQCQNLDGAFVIIPPLMPGPALLVDDIVDSAWTLTVLTALLRQAG
                     CPTVYPLALASTSVKN"
     gene            complement(23136..24476)
                     /gene="gntU"
                     /locus_tag="PN977_000319"
     CDS             complement(23136..24476)
                     /gene="gntU"
                     /locus_tag="PN977_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HDI6040400.1"
                     /translation="MTTLTLVLTAVGSVLLLLFLVMKARMHAFLALMVVSMGAGLFSG
                     MPLDKIAATMEKGMGGTLGFLAVVVALGAMFGKILHETGAVDQIAVKMLKSFGHSRAH
                     YAIGLAGLVCALPLFFEVAIVLLISVAFSMARHTGTNLVKLVIPLFAGVAAAAAFLVP
                     GPAPMLLASQMNADFGWMILIGLCAAIPGMIIAGPLWGNFISRYVELHIPDDISEPHL
                     GEGKMPSFGFSLSLILLPLVLVGLKTIAARFVPEGSTAYEWFEFIGHPFTAILVACLV
                     AIYGLAMRQGMPKDKVMEICGHALQPAGIILLVIGAGGVFKQVLVDSGVGPALGEALT
                     GMGLPIAITCFVLAAAVRIIQGSATVACLTAVGLVMPVIEQLNYSGAQMAALSICIAG
                     GSIVVSHVNDAGFWLFGKFTGATEAETLKTWTMMETILGTVGAIVGMIAFQLLS"
     gene            complement(24480..25007)
                     /gene="gntK"
                     /locus_tag="PN977_000320"
     CDS             complement(24480..25007)
                     /gene="gntK"
                     /locus_tag="PN977_000320"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HDI6040401.1"
                     /translation="MSTTNHDHHIYVLMGVSGSGKSAVASEVAHQLHAAFLDGDFLHP
                     RCNIEKMASGEPLNDDDRKPWLQALNDAAFAMQRTNKVSLIVCSALKKHYRDLLRAGN
                     PNLSFIYLKGDFDVIESRLKARKGHFFKTQMLVTQFETLQEPGADETDVLVVDIDQPL
                     EGVVASTIEVIKKGK"
     gene            complement(25146..26141)
                     /gene="gntR"
                     /locus_tag="PN977_000321"
     CDS             complement(25146..26141)
                     /gene="gntR"
                     /locus_tag="PN977_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate operon transcriptional repressor GntR"
                     /protein_id="HDI6040402.1"
                     /translation="MKKKRPVLQDVADRVGVTKMTVSRFLRNPEQVSVALRGKIAAAL
                     DELGYIPNRAPDILSNATSRAIGVLLPSLTNQVFAEVLRGIESVTDAHGYQTMLAHYG
                     YKPEMEQERLESMLSWNIDGLILTERTHTPRTLKMIEVAGIPVVELMDSKSPCLDIAV
                     GFDNFEAARQMTTAIIARGHRHIAYLGARLDERTIIKQKGYEQAMLDAGLVPYSVMVE
                     QSSSYSSGIELIRQARREYPQLDGVFCTNDDLAVGAAFECQRLGLKVPDDMAIAGFHG
                     HDIGQVMEPRLASVLTPRERMGSIGAERLLARIRGESVTPKMLDLGFTLSPGGSI"
     gene            complement(26365..27060)
                     /gene="yhhW"
                     /locus_tag="PN977_000322"
     CDS             complement(26365..27060)
                     /gene="yhhW"
                     /locus_tag="PN977_000322"
                     /EC_number="1.13.11.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004213399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quercetin 2,3-dioxygenase"
                     /protein_id="HDI6040403.1"
                     /translation="MIYLRKANERGHANHGWLDSWHTFSFANYYDPNFMGFSALRVIN
                     DDVIEAGQGFGTHPHKDMEILTYVLEGTVEHQDSMGNKEQVPAGEFQIMSAGTGIRHS
                     EYNPSSTERLHLYQIWIMPEENGITPRYEQRRFDAVQGKQLVLSPDARDGSLKVHQDM
                     ELYRWALLKDEQSVHQIAAERRVWIQVVKGNVTINGVKASTSDGLAIWDEQAISIHAD
                     SDSEVLLFDLPPV"
     gene            complement(27183..28220)
                     /locus_tag="PN977_000323"
     CDS             complement(27183..28220)
                     /locus_tag="PN977_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HDI6040404.1"
                     /translation="MVINCAFIGFGKSTTRYHLPYVLNRKDSWHVAHIFRRHAKPEEQ
                     APIYSHIHFTSDLDEVLNDPDVKLVVVCTHADSHFEYAKRALEAGKNVLVEKPFTPTI
                     AQAKELFALAKSKGLIVTPYQNRRFDSCFLTAKKAIESGKLGEIVEVESHFDYYRPVA
                     ETKPGLPQDGAFYGLGVHTMDQIISLFGRPDHVAYDIRSLRNKANPDDTFEAQLFYGD
                     LKAIVKTSHLVKIDYPKFIVHGKKGSFIKYGIDQQETSLKANIMPGEPGFAADDSVGV
                     LEYVNDEGVTVREEMKPEVGDYGRVYDALYQTITNGAPNYVKESEVLTNLEILERGFE
                     QASPSTVTLAK"
     gene            28554..29042
                     /gene="yhhY"
                     /locus_tag="PN977_000324"
     CDS             28554..29042
                     /gene="yhhY"
                     /locus_tag="PN977_000324"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HDI6040405.1"
                     /translation="MSEIVIRHAETRDYEAIRQIHAQPEVYYNTLQVPHPSEQMWLER
                     LTARPGIKQLVACIDGIVVGHLTIDVQQRPHRSHVADFGICVDSRWKNRGVASALMRE
                     MIEMCDNWLRVDRIELTVFVDNAPAIKVYKKFGFEIEGTGKKYALRNGEYVDAYYMAR
                     VK"
     gene            complement(29103..30848)
                     /gene="ggt"
                     /locus_tag="PN977_000325"
     CDS             complement(29103..30848)
                     /gene="ggt"
                     /locus_tag="PN977_000325"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HDI6040406.1"
                     /translation="MIKPTFLRRVAIAALFTGSCFSTVAAPPAPSPVSYGVEEDVFHP
                     VRAKQGMVASVDATATQVGVDILKEGGNAVDAAVAVGYALAVTHPQAGNLGGGGFMLI
                     RSKNGNTTAIDFREMAPAKATRDMFLDDQGNPDSKKSLTSHLASGTPGTVAGFSLALE
                     KYGTMPLNKVVQPAFKLARDGFIVNDALADDLKTYGSEVLPNHENSKAIFWKEGAPLK
                     KGDKLVQANLAKSLEMIAENGPDEFYKGTIAEQIAQEMQKNGGLITKEDLAAYKAVER
                     TPISGEYRGYQVYSMPPPSSGGIHIVQILNILENFDMKKYGFGSADAMQIMAEAEKYA
                     YADRSEYLGDPDFVKVPWQALTNKAYAKSIADQIDINKAKPSSEIRPGKLAPYESNQT
                     THYSVVDKDGNAVAVTYTLNTTFGTGIVAGESGILLNNQMDDFSAKPGVPNVYGLVGG
                     DANAVGPNKRPLSSMSPTIVVKDGKTWLVTGSPGGSRIITTVLQMVVNSIDYGMNVAE
                     ATNAPRFHHQWLPDELRVEKGFSPDTIKLLEAKGQKVALKEAMGSTQSIMVGPDGELY
                     GASDPRSVDDLTAGY"
     gene            30968..31408
                     /locus_tag="PN977_000326"
     CDS             30968..31408
                     /locus_tag="PN977_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2756 family protein"
                     /protein_id="HDI6040407.1"
                     /translation="MKRLLILTALLPFVGFAQPINTLNNPNQPGYQIPSQQRMQTQMQ
                     TQQIQQKGMLNQQLKTQTQLQQQHLENQINNNSQRVLQSQPGERNPARQQMLPNTNGG
                     MLNSNRNPDSSLNQQHMLPERRNGDMLNQPGTPQPDIPLKTIGP"
     gene            complement(31395..32138)
                     /gene="ugpQ"
                     /locus_tag="PN977_000327"
     CDS             complement(31395..32138)
                     /gene="ugpQ"
                     /locus_tag="PN977_000327"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HDI6040408.1"
                     /translation="MSNWPYPRIVAHRGGGKLAPENTLAAIDVGAKYGHKMIEFDAKL
                     SKDGEIFLLHDDNLERTSNGWGVAGDLNWQDLLRVDAGSWYSKAFKGEPLPLLSQVAE
                     RCRKHGMMANIEIKPTTGTGPLTGKMVALAARELWAGMTPPLLSSFEIDALDAAQQAV
                     PELPRGLLLDEWRDDWRELTARLGCVSIHLNHKLLDKARVMQLKDAGLRILVYTVNKP
                     QRAAELLRWGVDCICTDAIDVIGPNFTAQ"
     gene            complement(32135..33205)
                     /gene="ugpC"
                     /locus_tag="PN977_000328"
     CDS             complement(32135..33205)
                     /gene="ugpC"
                     /locus_tag="PN977_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol 3-phosphate ABC transporter ATP
                     binding protein UgpC"
                     /protein_id="HDI6040409.1"
                     /translation="MAGLKLQAVTKSWDGKTQVIKPLTLDVADGEFIVMVGPSGCGKS
                     TLLRMVAGLERVTTGDIWIDRKRVTEMEPKDRGIAMVFQNYALYPHMSVEENMAWGLK
                     IRGMGKQQIAERVKEAARILELDGLLKRRPRELSGGQRQRVAMGRAIVREPAVFLFDE
                     PLSNLDAKLRVQMRLELQQLHRRLKTTSLYVTHDQVEAMTLAQRVMVMNGGVAEQIGT
                     PVEVYEKPASLFVASFIGSPAMNLLAGRVNNEGTHFELDGGITLPLNGGYRQYAGRKM
                     TLGIRPEHIALSSQAEGGVPLVMDTLEILGADNLAHGRWGEQKLVVRLAHQERPTAGS
                     TLWLHLPENQLHLFDGETGQRV"
     gene            complement(33207..34052)
                     /gene="ugpE"
                     /locus_tag="PN977_000329"
     CDS             complement(33207..34052)
                     /gene="ugpE"
                     /locus_tag="PN977_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpE"
                     /protein_id="HDI6040410.1"
                     /translation="MIENRPWLTIFSHTMLILGIAVILFPLYVAFVAATLDKQEVYAA
                     PMTLIPGTHLLENIHNIWVNGVGTNSAPFWRMLLNSFVMAFSITLGKITVSMLSAFAI
                     VWFRFPLRNLFFWMIFITLMLPVEVRIFPTVEVIANLKMLDSYAGLTLPLMASATATF
                     LFRQFFMTLPDELVEAARIDGASPMRFFCDIVFPLSKTNLAALFVITFIYGWNQYLWP
                     LLIITDVDLGTTVAGIKGMIATGEGTTEWNSVMAAMLLTLIPPVVIVLVMQRAFVRGL
                     VDSEK"
     gene            complement(34049..34936)
                     /gene="ugpA"
                     /locus_tag="PN977_000330"
     CDS             complement(34049..34936)
                     /gene="ugpA"
                     /locus_tag="PN977_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000099274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpA"
                     /protein_id="HDI6040411.1"
                     /translation="MSSSRPVFRSRWLPYLLVAPQLIITVIFFIWPAGEALWYSLQSV
                     DPFGFSSQFVGLDNFVTLFHDSYYLDAFWTTIKFSTFVTVSGLLVSLFFAALVEYIVR
                     GSRFYQTLMLLPYAVAPAVAAVLWIFLFNPGRGLITHFLAEFGYDWNHAQNSGQAMFL
                     VVFASVWKQISYNFLFFYAALQSIPRSLIEAAAIDGAGPIRRFFKIALPLIAPVSFFL
                     LVVNLVYAFFDTFPVIDAATSGGPVQATTTLIYKIYREGFTGLDLASSAAQSVVLMFL
                     VIVLTVVQFRYVESKVRYQ"
     gene            complement(35034..36350)
                     /gene="ugpB"
                     /locus_tag="PN977_000331"
     CDS             complement(35034..36350)
                     /gene="ugpB"
                     /locus_tag="PN977_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     substrate-binding protein UgpB"
                     /protein_id="HDI6040412.1"
                     /translation="MKPLRYTASALALGLALMANAQAATTIPFWHSMEGELGKEVDSL
                     AQRFNAENPDYKIVPTYKGNYEQNLSAGIAAFRTGNAPAILQVYEVGTATMMASKAIK
                     PVYDVFKEAGIQFDESQFVPTVSGYYSDSKTGHLLSQPFNSSTPVLYYNKDAFKKAGL
                     DPEQPPKTWQDLADYAAKLKASGMKCGYASGWQGWIQLENFSAWNGLPFASKNNGFDG
                     TDAVLEFNKPEQVKHIAMLEEMNKKGDFSYVGRKDESTEKFYNGDCAMTTASSGSLAN
                     IREYAKFNYGVGMMPYDADAKDAPQNAIIGGASLWVMQGKDKETYTGVAKFLDFLAKP
                     ENAAEWHQKTGYLPITKAAYDLTREQGFYEKNPGADIATRQMLNKPPLPFTKGLRLGN
                     MPQIRVIVDEELESVWTGKKTPQQALDTAVERGNQLLRRFEKSTKS"
     gene            complement(36710..37456)
                     /locus_tag="PN977_000332"
     CDS             complement(36710..37456)
                     /locus_tag="PN977_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HDI6040413.1"
                     /translation="MHYAVVVMLELLCCIHAYRTGQERYWIFIIFCFPVIGCVAYFVI
                     VMLPETGADRHGHTLLMRLQDKLNPERHLRKLTEELAIAETNQNHYALANELVCLGRY
                     HEAVPHYQQALSGIFVHEAAMMLSLAQAQFAIQEFAACQQTLEDVMRYNPDFQSADGH
                     LLFARALAAQEKYADAESEFEVLISYYSGPQARIYYAEMLAKMSRLREANEQYIAVVD
                     TAKRSKPHYRKHHREWIKTANERLKQSVVQ"
     gene            complement(37575..38288)
                     /gene="livF"
                     /locus_tag="PN977_000333"
     CDS             complement(37575..38288)
                     /gene="livF"
                     /locus_tag="PN977_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivF"
                     /protein_id="HDI6040414.1"
                     /translation="MEKVMLSFDKVSAHYGKIQALHEVSLHINQGEIVTLIGANGAGK
                     TTLLGTLCGDPRATSGRIVFDDKDITDWQTAKIMREAVAIVPEGRRVFSRMTVEENLA
                     MGGFFAERDQFQERIKWVYELFPRLHERRVQRAGTMSGGEQQMLAIGRALMSNPRLLL
                     LDEPSLGLAPIIIQQIFDTIEQLREQGMTIFLVEQNANQALKLADRGYVLENGHVVLS
                     DTGDALLANEAVRSAYLGG"
     gene            complement(38290..39057)
                     /gene="livG"
                     /locus_tag="PN977_000334"
     CDS             complement(38290..39057)
                     /gene="livG"
                     /locus_tag="PN977_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000084170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivG"
                     /protein_id="HDI6040415.1"
                     /translation="MSQPLLSVNGLMMRFGGLLAVNNVNLELYPQEIVSLIGPNGAGK
                     TTVFNCLTGFYKPTGGTILLRDQHLEGLPGQQIARMGVVRTFQHVRLFREMTVIENLL
                     VAQHQQLKTGLFSGLLKTPSFRRAQSEALDRAATWLERIGLLEHANRQASNLAYGDQR
                     RLEIARCMVTQPEILMLDEPAAGLNPKETKELDELIAELRNHHNTTILLIEHDMKLVM
                     GISDRIYVVNQGTPLANGTPEQIRNNPDVIRAYLGEA"
     gene            complement(39054..40331)
                     /gene="livM"
                     /locus_tag="PN977_000335"
     CDS             complement(39054..40331)
                     /gene="livM"
                     /locus_tag="PN977_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     permease LivM"
                     /protein_id="HDI6040416.1"
                     /translation="MKPMHIAMALLSAAMFFVLAGVFMGVQLELDGTKLVVDTASEIR
                     WQWVFIGTAVVFFFQLLRPAFQKGLKSVSGPKFILPAIDGSTVKQKLFLVALLVLAVA
                     WPFMVSRGTVDIATLTMIYIILGLGLNVVVGLSGLLVLGYGGFYAIGAYTFALLNHYY
                     GLGFWTCLPIAGLMAAAAGFLLGFPVLRLRGDYLAIVTLGFGEIVRILLLNNTEITGG
                     PNGISQIPKPTFFGLEFSRTAREGGWDTFSNFFGLKYDPSDRVIFLYLVALLLVVLSL
                     FVINRLLRMPLGRAWEALREDEIACRSLGLSPRRIKLTAFTISAAFAGFAGTLFAARQ
                     GFVSPESFTFAESAFVLAIVVLGGMGSQFAVILAAILLVVSRELMRDFNEYSMLMLGG
                     LMVLMMIWRPQGLLPMTRPQLKLKNGAAKGEQA"
     gene            complement(40328..41254)
                     /gene="livH"
                     /locus_tag="PN977_000336"
     CDS             complement(40328..41254)
                     /gene="livH"
                     /locus_tag="PN977_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010436488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter permease LivH"
                     /protein_id="HDI6040417.1"
                     /translation="MSEQFLYFLQQMFNGVTLGSTYALIAIGYTMVYGIIGMINFAHG
                     EVYMIGSYVSFMIIAALMMLGIDTGWLLVAAGFVGAIVIASAYGWSIERVAYRPVRNS
                     KRLIALISAIGMSIFLQNYVSLTEGSRDVALPSLFNGQWVVGHSENFSASITTMQAVI
                     WIVTFLAMLALTIFIRYSRMGRACRACAEDLKMASLLGINTDRVIALTFVIGAAMAAV
                     AGVLLGQFYGVINPYIGFMAGMKAFTAAVLGGIGSIPGAMIGGLILGIAEALSSAYLS
                     TEYKDVVSFALLILVLLVMPTGILGRPEVEKV"
     gene            complement(41302..42411)
                     /gene="livK"
                     /locus_tag="PN977_000337"
     CDS             complement(41302..42411)
                     /gene="livK"
                     /locus_tag="PN977_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter substrate-binding protein LivK"
                     /protein_id="HDI6040418.1"
                     /translation="MKRNAKTIIAGMIALTISHTAMADDIKVAVVGAMSGPIAQWGDM
                     EFNGARQAIKDINAKGGIKGDKLVGVEYDDACDPKQAVAVANKIVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMISPGATNPELTQRGYQYIMRTAGLDSSQGPTAAKYIVETVK
                     PQRIAIIHDKQQYGEGLARSVQDGLKAANANVVFFDGITAGEKDFSALIARLKKENID
                     FVYYGGYYPEMGQMLRQARSVGLKTQFMGPEGVGNASLSNIAGDAAEGMLVTMPKRYD
                     QDPANQGIVDALKADKKDPSGPYVWITYAAVQSLATALERTGSDEPLALVKDLKANGA
                     NTVIGPLNWDEKGDLKGFDFGVFQWHADGSSTAAK"
     gene            42835..43218
                     /gene="panM"
                     /locus_tag="PN977_000338"
     CDS             42835..43218
                     /gene="panM"
                     /locus_tag="PN977_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase autocleavage activator
                     PanM"
                     /protein_id="HDI6040419.1"
                     /translation="MKLTIIRLENFSDQDRIDLQKIWPEYSPSSLQVDDNHRIYAARF
                     NERLLAAVRVTLSGTEGALDSLRVREVTRRRGVGQYLLEEVLRTNPGVSCWWMADAGV
                     EDRGVMTAFMRALGFTAQQGGWEKR"
     gene            complement(43406..44509)
                     /gene="livJ"
                     /locus_tag="PN977_000339"
     CDS             complement(43406..44509)
                     /gene="livJ"
                     /locus_tag="PN977_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     substrate-binding protein LivJ"
                     /protein_id="HDI6040420.1"
                     /translation="MNIKGKALLAGCIALAFSNMALAEDIKVAVVGAMSGPVAQYGDQ
                     EFTGAEQAVADINAKGGIKGNKLQIVKYDDACDPKQAVAVANKVVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMITPAATAPELTARGYQLILRTTGLDSDQGPTAAKYILEKVK
                     PQRIAIVHDKQQYGEGLARAVQDGLKKGNANVVFFDGITAGEKDFSTLVARLKKENID
                     FVYYGGYHPEMGQILRQARAAGLKTQFMGPEGVANVSLSNIAGESAEGLLVTKPKNYD
                     QVPANKPIVDAIKAKKQDPSGAFVWTTYAALQSLQAGLNQSDDPAEIAKYLKANSVDT
                     VMGPLSWDEKGDLKGFEFGVFDWHANGTATDAK"
     gene            complement(44781..45635)
                     /gene="rpoH"
                     /locus_tag="PN977_000340"
     CDS             complement(44781..45635)
                     /gene="rpoH"
                     /locus_tag="PN977_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HDI6040421.1"
                     /translation="MTDKMQSLALAPVGNLDSYIRAANAWPMLSADEERALAEKLHYH
                     GDLEAAKTLILSHLRFVVHIARNYAGYGLPQADLIQEGNIGLMKAVRRFNPEVGVRLV
                     SFAVHWIKAEIHEYVLRNWRIVKVATTKAQRKLFFNLRKTKQRLGWFNQDEVEMVARE
                     LGVTSKDVREMESRMAAQDMTFDLSSDDDSDSQPMAPVLYLQDKSSNFADGIEDDNWE
                     EQAANRLTDAMQGLDERSQDIIRARWLDEDNKSTLQELADRYGVSAERVRQLEKNAMK
                     KLRAAIEA"
     gene            complement(45880..46938)
                     /gene="ftsX"
                     /locus_tag="PN977_000341"
     CDS             complement(45880..46938)
                     /gene="ftsX"
                     /locus_tag="PN977_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsX"
                     /protein_id="HDI6040422.1"
                     /translation="MNKRDAINHIRQFGGRLDRFRKSVGGSGDGGRNAPKRAKSSPKP
                     VNRKTNVFNEQVRYAFHGALQDLKSKPFATFLTVMVIAISLTLPSVCYMVYKNVNQAA
                     TQYYPSPQITVYLQKTLDDDAAAGVVAQLQAEQGVEKVNYLSREDALGEFRNWSGFGG
                     ALDMLEENPLPAVAVVIPKLDFQGTESLNTLRDRITQINGIDEVRMDDSWFARLAALT
                     GLVGRVSAMIGVLMVAAVFLVIGNSVRLSIFARRDSINVQKLIGATDGFILRPFLYGG
                     ALLGFSGALLSLILSEILVLRLSSAVAEVAQVFGTKFDINGLSFDECLLLLLVCSMIG
                     WVAAWLATVQHLRHFTPE"
     gene            complement(46931..47599)
                     /gene="ftsE"
                     /locus_tag="PN977_000342"
     CDS             complement(46931..47599)
                     /gene="ftsE"
                     /locus_tag="PN977_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HDI6040423.1"
                     /translation="MIRFEHVSKAYLGGRQALQGVTFHMQPGEMAFLTGHSGAGKSTL
                     LKLICGIERPSAGKIWFSGHDITRLKNREVPFLRRQIGMIFQDHHLLMDRTVYDNVAI
                     PLIIAGASGDDIRRRVSAALDKVGLLDKAKNFPIQLSGGEQQRVGIARAVVNKPAVLL
                     ADEPTGNLDDALSEGILRLFEEFNRVGVTVLMATHDINLISRRSYRMLTLSDGHLHGG
                     VGHE"
     gene            complement(47602..49140)
                     /gene="ftsY"
                     /locus_tag="PN977_000343"
     CDS             complement(47602..49140)
                     /gene="ftsY"
                     /locus_tag="PN977_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HDI6040424.1"
                     /translation="MAKEKKRGFFSWLGFGQKEQTPEKETEVQNEQPVVEEIVPAQEP
                     VKASEQTVEEQPQAHTEAEAETFAADVVEVTEQVAESEKAQPEAVAEPEVITEPETIV
                     EETPASVIEPEVTPEPVAIEREELPLPEDVNAEEVSPEEWQAEAETLEIVEAAEEEAA
                     KEDITDEELEAQALAAEAAEEAVMVVSPAEEEQPVEEIAQEQEKPTKEGFFARLKRSL
                     LKTKENLGSGFISLFRGKKIDDDLFEELEEQLLIADVGVETTRKIITNLTEGASRKQL
                     RDAEALYGLLKEEMGEILAKVDEPLNVEGKTPFVILMVGVNGVGKTTTIGKLARQFEQ
                     QGKSVMLAAGDTFRAAAVEQLQVWGQRNNIPVIAQHTGADSASVIFDAIQAAKARNID
                     VLIADTAGRLQNKSHLMEELKKIVRVMKKLDVEAPHEVMLTIDASTGQNAVSQAKLFH
                     EAVGLTGITLTKLDGTAKGGVIFSVADQFGIPIRYIGVGERIEDLRPFKADDFIEALF
                     ARED"
     gene            49290..49886
                     /gene="rsmD"
                     /locus_tag="PN977_000344"
     CDS             49290..49886
                     /gene="rsmD"
                     /locus_tag="PN977_000344"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase"
                     /protein_id="HDI6040425.1"
                     /translation="MKKPNHSGSGQIRIIGGQWRGRKLPVPDSPGLRPTTDRVRETLF
                     NWLAPVIVDAQCLDCFAGSGALGLEALSRYAAGATLIEMDRAVSQQLIKNLATLKAGN
                     ARVVNSNAMSFLAQKGTPHNIVFVDPPFRRGLLEETINLLEDNGWLADEALIYVESEV
                     ENGLPTVPANWSLHREKVAGQVAYRLYQREAQGESDVD"
     gene            49876..50145
                     /locus_tag="PN977_000345"
     CDS             49876..50145
                     /locus_tag="PN977_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1145 family protein"
                     /protein_id="HDI6040426.1"
                     /translation="MLINIGRLLMLCVWGFLILNLVHPFPRPLNIFVNVALIFTVLMH
                     GMQLALLKSTLPKDGPQMTTAEKVRIFLFGVFELLVWQKKFKVKK"
     gene            complement(50148..50507)
                     /locus_tag="PN977_000346"
     CDS             complement(50148..50507)
                     /locus_tag="PN977_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2500 domain-containing protein"
                     /protein_id="HDI6040427.1"
                     /translation="MSKPPLFFIVIIGLIIVAASFRFMQQRREKADNDMAPLQQKLVV
                     VSNKREKPINDRRSRQQEVTPAGTSMRYEASFKPQSGGMEQTFRLDAQQYHALTVGDK
                     GTLSYKGTRFVSFIGEQ"
     gene            50648..51274
                     /locus_tag="PN977_000347"
     CDS             50648..51274
                     /locus_tag="PN977_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysoplasmalogenase"
                     /protein_id="HDI6040428.1"
                     /translation="MLWSFIAVCLSAWLSVDASYRGPTWQRWVFKPLTLLLLLLLAWQ
                     APMFDAISYLVLAGLCASLLGDALTLLPRQRLMYAIGAFFLSHLLYTIYFASQMTLSF
                     FWPLPLVLLVLGALLLAIIWTRLEEYRWPICTFIGMTLVMVWLAGELWFFRPTAPALS
                     AFVGASLLFISNFVWLGSHYRRRFRADNAIAAACYFAGHFLIVRSLYL"
     gene            51348..53546
                     /gene="zntA"
                     /locus_tag="PN977_000348"
     CDS             51348..53546
                     /gene="zntA"
                     /locus_tag="PN977_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(II)/Cd(II)/Pb(II) translocating P-type ATPase
                     ZntA"
                     /protein_id="HDI6040429.1"
                     /translation="MSTPDNHGKKAPQFAAFKPLTTVQNTNDCCCDGACSSTPTLSES
                     VSGTRYSWKVSGMDCAACARKVENAVRQLAGVNQVQVLFATEKLVVDADNDIRAQVES
                     AVQKAGYSLRDEQTTDEPQASRLKENLPLITLIVMMAISWGLEQFNHPFGQLAFIATT
                     LVGLYPIARQALRLIKSGSYFAIETLMSVAAIGALFIGATAEAAMVLLLFLIGERLEG
                     WAASRARQGVSALMALKPETATRLRNGEREEVAINSLRPGDVIEVAAGGRLPADGKLL
                     SPFASFDESALTGESIPVERATGDKVPAGATSVDRLVTLEVLSEPGASAIDRILKLIE
                     EAEERRAPIERFIDRFSRIYTPAIMAVALLVTLVPPLLFAASWQEWIYKGLTLLLIGC
                     PCALVISTPAAITSGLAAAARRGALIKGGAALEQLGRVTQVAFDKTGTLTVGKPRVTA
                     IHPATGISESELLTLAAAVEQGATHPLAQAIVREAQVAELAIPTAQSQRALVGSGIEA
                     QVNGERVLICAAGKHPADAFAGLINELESAGQTVVLVVRNDDVLGVIALQDTLRADAA
                     TAISELNALGVKGVILTGDNPRAAAAIAGELGLEFKAGLLPEDKVKAVTELNQHAPLA
                     MVGDGINDAPAMKAAAIGIAMGSGTDVALETADAALTHNHLRGLVQMIELARATHANI
                     RQNITIALGLKGVFLVTTLLGMTGLWLAVLADTGATVLVTANALRLLRRK"
     gene            complement(53579..53887)
                     /locus_tag="PN977_000349"
     CDS             complement(53579..53887)
                     /locus_tag="PN977_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000385055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6040430.1"
                     /translation="MDYLIPVCLALLGLLAIFTPNWLIPVFIVVGGWLGVLAWGWLWL
                     MIFLPLLNGVGLAYALWLMFKRPSIASRLVAACCVISVILMLALYYAFLTMPPFPPQH
                     "
     gene            complement(54102..54347)
                     /gene="tusA"
                     /locus_tag="PN977_000350"
     CDS             complement(54102..54347)
                     /gene="tusA"
                     /locus_tag="PN977_000350"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA"
                     /protein_id="HDI6040431.1"
                     /translation="MTDLFSSPDHTLDALGLRCPEPVMMVRKTVRNMQPGETLLIIAD
                     DPATTRDIPGFCTFMEHELVAKETDGLPYRYLIRKSG"
     gene            54568..55233
                     /locus_tag="PN977_000351"
     CDS             54568..55233
                     /locus_tag="PN977_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine/7-aminomethyl-7-
                     deazaguanine transporter"
                     /protein_id="HDI6040432.1"
                     /translation="MNVFSQTQRYKALFWLSLFHLLVITSSNYLVQLPVSIFGFHTTW
                     GAFSFPFIFLATDLTVRIFGAPLARRIIFAVMIPALLISYVISSLFYMGSWQGFGALA
                     HFNLFVARIATASFMAYALGQILDVHVFNRLRQSRRWWLAPTASTLFGNVSDTLAFFF
                     IAFWRSPDAFMAEHWMEIALVDYCFKVLISIVFFLPMYGVLLNMLLKRLADKSEINAL
                     QAS"
     gene            55306..55863
                     /gene="dcrB"
                     /locus_tag="PN977_000352"
     CDS             55306..55863
                     /gene="dcrB"
                     /locus_tag="PN977_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417929.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage sensitivity protein DcrB"
                     /protein_id="HDI6040433.1"
                     /translation="MRNLVKYVGIGLLVMGLAACDDKDTNATAQGSVAESNATGNPVN
                     LLDGKLSFSLPADMTDQSGKLGTQANNMHVWSDATGQKAVIVIMGDDPKEDLAVLAKR
                     LEDQQRSRDPQLQVVTNKAIELKGHKMQQLDSIISAKGQTAYSSVILGNVGNQLLTMQ
                     ITLPADDQQKAQTTAENIINTLVIQ"
     gene            complement(55867..57117)
                     /locus_tag="PN977_000353"
     CDS             complement(55867..57117)
                     /locus_tag="PN977_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312349.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDI6040434.1"
                     /translation="MKHCCKNVVILMPEPVAEPALNGLRLNLRIVSIVMFNFASYLTI
                     GLPLAVLPGYVHDVMGFSAFWAGLVISLQYFATLLSRPHAGRYADLLGPKKIVVFGLC
                     GCFLSGLGYLTAGLTASLPVISLLLLCLGRVILGIGQSFAGTGSTLWGVGVVGSLHIG
                     RVISWNGIVTYGAMAMGAPLGVVFYHWGGLQALALIIMGVALVAILLAIPRPMVKASK
                     GKPLPFRAVLGRVWLYGMALALASAGFGVIATFITLFYDAKGWDGAAFALTLFSCAFV
                     GTRLLFPNGINRIGGLNVAMICFSVEIIGLLLVGVATMPWMAKIGVLLAGAGFSLVFP
                     ALGVVAVKAVPQQNQGAALATYTVFMDLSLGVTGPLAGLVMSWAGVPVIYLAAAGLVA
                     IALLLTWRLKKRPPVEVPEAISSS"
     gene            57216..58265
                     /locus_tag="PN977_000354"
     CDS             57216..58265
                     /locus_tag="PN977_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HDI6040435.1"
                     /translation="METPQPDKTGMHILLKLASLVVILAGIHAAADIIVQLLLALFFA
                     IVLNPLVTWFIRRGVQRPVAITIVVVVMLIALTALVGVLAASFNEFISMLPKFNKELT
                     RKLFKLQEMLPFLNLHMSPERMLQRMDSEKMVTFTTALMTGLSGAMASVLLLVMTVVF
                     MLFEVRHVPYKMRFALNNPQIHIAGLHRALKGVSHYLALKTLLSLWTGVIVWLGLALM
                     GVQFALMWAVLAFLLNYVPNIGAVISAVPPMIQVLLFNGVYECILVGALFLVVHMVIG
                     NILEPRMMGHRLGMSTMVVFLSLLIWGWLLGPVGMLLSVPLTSVCKIWMETTKGGSKL
                     AILLGPGRPKSRLPG"
     gene            58320..58907
                     /gene="acpT"
                     /locus_tag="PN977_000355"
     CDS             58320..58907
                     /gene="acpT"
                     /locus_tag="PN977_000355"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase AcpT"
                     /protein_id="HDI6040436.1"
                     /translation="MYRIVLGKVSTLSAAPLPPGLREQAPQGPRRERWLAGRALLSHT
                     LSPLPEIIYGEQGKPAFAPETPLWFNLSHSGDDIALLLSDEGEVGCDIEVIRPRANWR
                     WLANAVFSLGEHAEMDAVHPDQQLEMFWRIWTRKEAIVKQRGGSAWQIVSVDSTYNSS
                     LSVSHCQLENLSLAICTPTPFTLTADSVQWIDSVN"
     gene            59018..60592
                     /gene="nikA"
                     /locus_tag="PN977_000356"
     CDS             59018..60592
                     /gene="nikA"
                     /locus_tag="PN977_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter, nickel/metallophore
                     periplasmic binding protein"
                     /protein_id="HDI6040437.1"
                     /translation="MFSTLRRTLFALLACASFIVHAAAPDEITTAWPVNVGPLNPHLY
                     TPNQMFAQSMVYEPLVKYQADGSVIPWLAKSWTHSEDGKTWTFTLRDDVKFSNGEPFD
                     AEAAAENFRAVLDNRQRHAWLELANQIVDVKALSKNELQITLKSAYYPFLQELALPRP
                     FRFIAPSQFKNHETMNGIKAPIGTGPWVLQESKLNQYDVFVRNENYWGEKPAIKKITF
                     NVIPDPTTRAVAFETGDIDLLYGNEGLLPLDTFARFSQNSAYHTQLSQPIETVMLALN
                     TAKAPTNELAVREALNYAVNKKSLIDNALYGTQQVADTLFAPSVPYANLGLKPRQYDP
                     QKAKALLEQAGWTLPAGKDIREKNGQPLRIELSFIGTDALSKSMAEIIQADMRQIGAD
                     VTLIGEEESSIYARQRDGRFGMIFHRTWGAPYDPHAFLSSMRVPSHADFQAQQGLADK
                     PLIDKEIGEVLATHDETQRQALYRDILTRLHNEAVYLPISYISMMVVSKPELGNIPYA
                     PIATEIPFEQIKPVKP"
     gene            60592..61536
                     /gene="nikB"
                     /locus_tag="PN977_000357"
     CDS             60592..61536
                     /gene="nikB"
                     /locus_tag="PN977_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikB"
                     /protein_id="HDI6040438.1"
                     /translation="MLRYVLRRFLLLIPMVLAASVIIFLMLRLGTGDPALDYLRLSNL
                     PPTPEMLASTRTMLGLDQPLYVQYGTWLWKALHLDFGISFASQRPVLDDMLTFLPATL
                     ELAGAALVLILLTSVPLGIWAARHRDRLPDFAVRFIAFLGVSMPNFWLAFLLVMAFSV
                     YLQWLPAMGYGGWQHIILPAVSIAFMSLAINARLLRASMLDVAGQRHVTWARLRGLND
                     KQTERRHILRNASLPMITAVGMHIGELIGGTMIIENIFAWPGVGRYAVSAIFNRDYPV
                     IQCFTLMMVVVFVVCNLIVDLLNAALDPRIRRHEGAHA"
     gene            61533..62366
                     /gene="nikC"
                     /locus_tag="PN977_000358"
     CDS             61533..62366
                     /gene="nikC"
                     /locus_tag="PN977_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikC"
                     /protein_id="HDI6040439.1"
                     /translation="MNFFLSSRWSVRLALIIIALLALIALTSQWWLPYDPQAIDLPSR
                     LLSPDAQHWLGTDHLGRDIFSRLMAATRVSLGSVMACLLLVLTLGLVIGGSAGLIGGR
                     VDQATMRVADMFMTFPTSILSFFMVGVLGTGLTNVIIAIALSHWAWYARMVRSLVISL
                     RQREFVLASRLSGAGHVRVFVDHLAGAVIPSLLVLATLDIGHMMLHVAGMSFLGLGVT
                     APTAEWGVMINDARQYIWTQPLQMFWPGLALFISVMAFNLVGDALRDHLDPHLVTEHA
                     H"
     gene            62366..63130
                     /gene="nikD"
                     /locus_tag="PN977_000359"
     CDS             62366..63130
                     /gene="nikD"
                     /locus_tag="PN977_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikD"
                     /protein_id="HDI6040440.1"
                     /translation="MPQQIELRNIALQAAQPLVHGVSLTLKRGRVLALVGGSGSGKSL
                     TCAATLGILPAGVRQTAGEILADGKPVSPCALRGIKIATIMQNPRSAFNPLHTMHTHA
                     RETCLALGKPADDATLTAAIEAVGLENAARVLKLYPFEMSGGMLQRMMIAMAVLCESP
                     FIIADEPTTDLDVVAQARILDLLESIMQKQAPGMLLVTHDMGVVARLADDVAVMSHGK
                     IVEQGDVETLFNAPKHAVTRSLVSAHLALYGMELAS"
     gene            63127..63933
                     /gene="nikE"
                     /locus_tag="PN977_000360"
     CDS             63127..63933
                     /gene="nikE"
                     /locus_tag="PN977_000360"
                     /EC_number="7.2.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikE"
                     /protein_id="HDI6040441.1"
                     /translation="MTLLNVSDLSHHYAHGGFSGKHQHQAVLNNVSLTLKSGETVALL
                     GRSGCGKSTLARLLVGLESPSQGNISWRGEPLAKLNRAQRKAFRRDIQMVFQDSISAV
                     NPRKTVREILREPMRHLLSLKKAEQLARASEMLKAVDLDDSVLDKRPPQLSGGQLQRV
                     CLARALAVEPKLLILDEAVSNLDLVLQAGVIRLLKKLQQQFGTACLFITHDLRLVERF
                     CQRVMVMDNGQIVETQAVGDKLTFSSDAGRVLQNAVLPAFPVRRRTTEKV"
     gene            63939..64340
                     /gene="nikR"
                     /locus_tag="PN977_000361"
     CDS             63939..64340
                     /gene="nikR"
                     /locus_tag="PN977_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-responsive transcriptional regulator
                     NikR"
                     /protein_id="HDI6040442.1"
                     /translation="MQRITITLDDDLLETLDSLSQRRGYNNRSEAIRDILRSALAQEA
                     TQQHGTQGFAVLSYVYEHEKRDLASRIVSTQHHHHDLSVATLHVHINHDDCLEIAVLK
                     GDMGDVQHFADDVIAQRGVRHGHLQCLPKED"
     gene            64539..65285
                     /locus_tag="PN977_000362"
     CDS             64539..65285
                     /locus_tag="PN977_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDI6040443.1"
                     /translation="MKSLSKSSQIPLYQQVVEWIRESIYTGDLVEDDRIPSEYQIMDM
                     LEVSRGTVKKAVAQLVKEGVLIQVQGKGTFVKKENVAYPLGEGLLSFAESLESQKIHF
                     TTEVITSRIEPANRYVAEKLRITPGQDILYLERLRSIGDEKAMLIENRINIELCPGIV
                     EIDFNQHNLFPTIESLSQRKIRYSESRYAARLIGNERGHFLDISEDAPVLHLEQLVFF
                     SRELPVEFGNVWLKGNKYYLGTVLQRREVS"
     gene            65310..65783
                     /locus_tag="PN977_000363"
     CDS             65310..65783
                     /locus_tag="PN977_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDI6040444.1"
                     /translation="MQGIQFQENYIQRLPAGLSVEQIIRQLAQPLVAAELVVPDFANH
                     VLKREATYPTGLPTEPPCVAIPHTDHKHVRHNAIAVGILPEPVEFADMGGDPDPVPVR
                     VIFLLALSESNKQLNALGWIMEMIQDTPFMQALLTMETTEIHTVILNKMNERGEI"
     gene            65780..66061
                     /locus_tag="PN977_000364"
     CDS             65780..66061
                     /locus_tag="PN977_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDI6040445.1"
                     /translation="MSQTILFVCATGIATSTAVTEKVMEYCKEHGLNVNYSQTNVASL
                     PGNTDGVALVVSTTKVPYELDVPVVNGLPIITGIGEEKVLAQIVSILKK"
     gene            66135..67493
                     /locus_tag="PN977_000365"
     CDS             66135..67493
                     /locus_tag="PN977_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HDI6040446.1"
                     /translation="MNDIAHTLYNIVQYILGFGPTVMLPLVLFILALCFKVKPAKALR
                     SSLTVGIGFVGIYAIFDILTSNVGPAAQAMVERTGINLPVVDLGWPPLSAITWGSPIA
                     PFVIPLTILINVAMLALNKTRTVDVDMWNYWHFALAGTLVYYSTGSLFFGLLAAAIAA
                     VVVLKLADWSAPLVQKYFGLEGISLPTLSSVVFFPVGLLVDKIIDHIPGLNRIHIDPE
                     TVQKKFGIFGEPMMVGTILGILLGVIAGYDFKKVLLLGISIGGVMFILPRMVRILMEG
                     LLPLSEAIKKYLNAKYPDRDDLYIGLDIAVAVGNPAIISTALLLTPISVFIAFVLPGN
                     EVLPLGDLANLAVMASMIALASRGNIFRTVLAAIPVIIADLWIATKIAPFITGMAKDV
                     NFKFAEGSSGQVSSFLDGGNPFRFWLLEIFNGNLIAIGLVPVIALVLYGIFRMTRSTV
                     YA"
     gene            67486..68994
                     /locus_tag="PN977_000366"
     CDS             67486..68994
                     /locus_tag="PN977_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase"
                     /protein_id="HDI6040447.1"
                     /translation="MPDNSAAIVIDIGTTNCKVTCFSCLDATTLGAHKFVTAKQISPQ
                     GDVDFDIDALWQEVRQAIAQLSAASPLPVRRISIASFGESGVFLDEHGEILTPMLAWY
                     DRRGEEYLATLSEADSAALYDICGLPLHSNYSAFKMRWLLEHYPLRNRRGLRWLHAPE
                     VLLWRLTGEQRTDITLASRTLCLDVRKGEWSAKAAALLHVPCSAFAPLVQPGEHAGWV
                     SESLCKTLGFLQPVSVTLAGHDHMVGARALQMTPGDILNSTGTTEGILQLDTQPTLDE
                     HAKRDKLANGCYSLANQFTLFASLPVGGFALEWLRNTFRLTDEEIAASLTRGHADYLA
                     GNWSLDDIPVFIPHLRGSGSPYKNRHTRGLFYGLGDTLNIDMLIASVSLGLTMEFANC
                     FACFNVPGTSALKVIGPATRNPLWLQLKADILQRPVEAIAFNEAVSVGALLTAAPDIP
                     PPQVTIAQRLLPNRARYHQLQRYQHKWKSWYQLKLQQEGVMPLHHREEHYVE"
     gene            68984..69253
                     /locus_tag="PN977_000367"
     CDS             68984..69253
                     /locus_tag="PN977_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HDI6040448.1"
                     /translation="MLSKTVEVRNSTGLHARPAACLAKAAKKYSCKVTLHYEGNDINA
                     TSMMNIMRAGIKGGKTVEIRCEGDDENEAIQTLTTLFRDRFGEAE"
     gene            69285..70145
                     /locus_tag="PN977_000368"
     CDS             69285..70145
                     /locus_tag="PN977_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase"
                     /protein_id="HDI6040449.1"
                     /translation="MPLVNGRILLDRIQEKRVLAGAFNTTNLETTISILNAIERSGLP
                     NFIQIAPTNAQLSGYDYIYEIVKRHADKMDVPVSLHLDHGKTQEDVKQAVRAGFTSVM
                     IDGAAFSFEENIAFTQEAVDFCKSYGVPVEAELGAILGKEDDHVSEADCKTEPEKVKT
                     FVERTGCDMLAVSIGNVHGLDDIPRIDIPLLKRIAEVSPVPLVIHGGSGIAPEILRSF
                     VNYRVAKVNIASDLRKAFITAVGKAYVNNHNEANLARVMASAKNAVEEDVYSKILMMN
                     EGHRLIRKAS"
     gene            complement(70195..70554)
                     /locus_tag="PN977_000369"
     CDS             complement(70195..70554)
                     /locus_tag="PN977_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HDI6040450.1"
                     /translation="MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSD
                     SIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKTFTLRYPESLSERLNNAAAQQQV
                     SVNTYIIETLNERLNHL"
     gene            complement(70551..70838)
                     /locus_tag="PN977_000370"
     CDS             complement(70551..70838)
                     /locus_tag="PN977_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001443258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HDI6040451.1"
                     /translation="MRTCHENPGTGFTEETKNTLDQIFKSPVPQGIKWSDIESLVKAL
                     GGEIKEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESVRDWLLSIGVKP"
     gene            complement(70898..72022)
                     /locus_tag="PN977_000371"
     CDS             complement(70898..72022)
                     /locus_tag="PN977_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDI6040452.1"
                     /translation="MRHLRNIFNLGIKELRSLLGDKAMLTLIVFSFTVSVYSSATVTP
                     GSLNLAPIAIADMDQSQLSNRIVNSFYRPWFLPPEMITADEMDAGLDAGRYTFAINIP
                     PNFQRDVLAGRQPDIQVNVDATRMSQAFTGNGYIQNIINGEVNSFVARYRDNSEPLVS
                     LETRMRFNPNLDPAWFGGVMAIINNITMLAIVLTGSALIREREHGTVEHLLVMPITPF
                     EIMMAKVWSMGLVVLVVSGLSLVLMVKGVLGVPIEGSIPLFMLGVALSLFATTSIGIF
                     MGTIARSMPQLGLLVILVLLPLQMLSGGSTPRESMPQMVQDIMLTMPTTHFVSLAQAI
                     LYRGAGFEIVWPQFLTLMAIGGAFFTIALLRFRKTIGTMA"
     gene            complement(72022..74757)
                     /gene="rbbA"
                     /locus_tag="PN977_000372"
     CDS             complement(72022..74757)
                     /gene="rbbA"
                     /locus_tag="PN977_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated ATPase/putative transporter
                     RbbA"
                     /protein_id="HDI6040453.1"
                     /translation="MTHLELVPVPPVAQLAGVSQHYGKTVALNNITLDIPARCMVGLI
                     GPDGVGKSSLLSLISGARVIEQGNVMVLGGDMRDPKHRRDVCPRIAWMPQGLGKNLYH
                     TLSVYENVDFFARLFGHDKAEREVRINDLLTSTGLAPFRDRPAGKLSGGMKQKLGLCC
                     ALIHDPELLILDEPTTGVDPLSRAQFWDLIDSIRQRQSNMSVLVATAYMEEAERFDWL
                     VAMNAGEVLATGSAEELRQQTQSATLEEAFINLLPQAQRQAHQAVVIPPYQPENAEIA
                     IEARDLTMRFGSFVAVDHVNFRIPRGEIFGFLGSNGCGKSTTMKMLTGLLPASEGEAW
                     LFGQPVDPKDIDTRRRVGYMSQAFSLYNELTVRQNLELHARLFHIPEAEIPARVAEMS
                     ERFKLNDVEDVLPESLPLGIRQRLSLAVAVIHRPEMLILDEPTSGVDPVARDMFWQLM
                     VDLSRQDKVTIFISTHFMNEAERCDRISLMHAGKVLASGTPQELVEKRGAASLEEAFI
                     AYLQEAAGQSNEAEAPPVIHDTTHAPRQGFSLRRLFSYSRREALELRRDPVRSTLALM
                     GTVILMLIMGYGISMDVENLRFAVLDRDQTVSSQAWTLNLSGSRYFIEQPPLTSYDEL
                     DRRMRAGDITVAIEIPPNFGRDIARGTPVELGVWIDGAMPSRAETVKGYVQAMHQSWL
                     QDVASRQSTPASQSGLMNIETRYRYNPDVKSLPAIVPAVIPLLLMMIPSMLSALSVVR
                     EKELGSIINLYVTPTTRSEFLLGKQLPYIGLGMLNFFLLCALSVFVFGVPHKGSFLTL
                     TLAALLYIIIATGMGLLISTFMKSQIAAIFGTAIITLIPATQFSGMIDPVASLEGPGR
                     WIGEVYPTSHFLTIARGTFSKALDLTDLWQLFIPLLIAIPLVMGLSILLLKKQEG"
     gene            complement(74754..75821)
                     /locus_tag="PN977_000373"
     CDS             complement(74754..75821)
                     /locus_tag="PN977_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HDI6040454.1"
                     /translation="MDKSKRHLAWWVVGLLAVAAIVAWWLLRPAGVPEGFAVSNGRIE
                     ATEVDIASKIAGRIDTILVKEGQFVREGEVLAKMDTRVLQEQRLEAIAQIKEAQSAIA
                     AAQALLEQRQSETRAAQSLVNQRQAELDSVAKRHTRSRSLAQRGAISAQQLDDDRAAA
                     ESARAALESAKAQVSASKAAIEAARTNIIQAQTRVEAAQATERRIAADIDDSELKAPR
                     DGRVQYRVAEPGEVLAAGGRVLNMVDLSDVYMTFFLPTEQAGTLKLGGEARLILDAAP
                     DLRIPATISFVASVAQFTPKTVETSDERLKLMFRVKARIPPELLQQHLEYVKTGLPGV
                     AWVRVNEELPWPDDLVVRLPQ"
     gene            complement(76187..77809)
                     /locus_tag="PN977_000374"
     CDS             complement(76187..77809)
                     /locus_tag="PN977_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HDI6040455.1"
                     /translation="MKIGTVAGTNDSITTIATNDMVQEHVTNFTKELFGYIANGIGDD
                     ISSIARTILGEVVEKIDDWQIERFQQSFQDDKISFTIETNHSEKYSMLSGMRAHILRR
                     NNSYQFIVTINSKNYGCPLDDTDIDWCSIVYLLNNMTVNDNANDVVVTESYKPIWDWK
                     ISQYNVFDIKFETIIKPQFADRIYFSNCSPVDPTSTRPTYFGDTDGSVGAVLYALFAT
                     GHLGIMAEGENFLSQLLNIEDEVLNVLLRENFNEQLDTNVNTIISILNRRDVVLESLQ
                     PYLVINKGAVTPCTFLGDQTGDRFSNICGDQFIIDLLKRIMSINENFHVLAGNHETNC
                     NGNYMQNFTRMKPLDEDTYAGIKDYPVCFYDPKYKIMANHHGITFDDQRKRYIIGPIT
                     VSIDEMTNALDPVELAAIINKKHHIIINGKKFKTSRAISCRSFNRYFSVSTDYRPKLE
                     ALLACSQMLGINQVVAHNGNGGRERIGETGTVLGLNARDSKHAGRMFSMHNCQINPGA
                     GPEITTPWKSYQHEKNKNGLMPLIRRRTMLQL"
     gene            complement(78071..79660)
                     /locus_tag="PN977_000375"
     CDS             complement(78071..79660)
                     /locus_tag="PN977_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HDI6040456.1"
                     /translation="MKVFHNIFKHISSNHQDKHSDKVNSHQHHGKVDKKHRSKIVEID
                     KLDKHLQIDNDFGLHIIYFLQHGCWNVTEHSSQIEKIWFYNSEPSIDIKEYNSFADNT
                     TDTFIFTIIPDNNHVIKLSSPITVTIERKDGYYFINFSGDKSDIIYKVDGFSIIERNF
                     FTLLSGNFKPDWRWAVSKETFTKEKFDSYVESVFSKIDFYKQCGVINPQNPNTAYFGD
                     TDGRVGAVLYALLVSGHIGIREKGWSLLCELLKHEDMASFAYENKKLKKLFTLLDTRD
                     MILNELHQHVFLKGDAITPCIFLGDHTGDRFSTIFGDKYILTLLNSMRNMEGNKDSRI
                     NKNVVVLAGNHEINFNGNYSARLANHKLSSGDTYNLIKTLDVCNYDSETKVLTSHHGI
                     IRNEENECYCLGALQVPFNQMKDPIDPEELANIFNKKHKQHMDDRLIHLIRSNAIAST
                     PVYDNYFNNTTAFRPKPEDIFKCGQTLKKSKQKYGHYGLGVDQHQKIDNYTMGLNSWK
                     IAPNERGDKKGVPGLSCFQAH"
     gene            80043..81095
                     /locus_tag="PN977_000376"
     CDS             80043..81095
                     /locus_tag="PN977_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417948.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2776 domain-containing protein"
                     /protein_id="HDI6040457.1"
                     /translation="MNIYIGWLFKLIPLIMGLICIALGGFVLESSGQSEYFVAGHVLI
                     SLAAICLALFTTAFIIISQLTRGVNTFYNTLFPIIGYAGSIITMIWGWALLAGNDVMA
                     DEFVAGHVIFGVGMIAACVSTVAASSGHFLLIPKNAAGSKSDGTPVQAYSSLIGNCLI
                     AVPVLLTLLGFIWSITLLRSADITPHYVAGHVLLGLTAICACLIGLVATIVHQTRNTF
                     SSKEHWLWCYWVIILGSITVLQGIYVLVSSDASVRLAPGIILICLGMICYSIFSKVWL
                     LALVWRRTCSLANRIPMIPVFTCLFCLFLASFLAEMAQTDIGYFIPSRVLVGLGAVCF
                     TLFSIVSILEAGSAKK"
     gene            complement(81410..82612)
                     /locus_tag="PN977_000377"
     CDS             complement(81410..82612)
                     /locus_tag="PN977_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HDI6040458.1"
                     /translation="MERFDAIIIGAGAAGMFCSALAGQAGRRVLLIDNGKKPGRKILM
                     SGGGRCNFTNLYVEPGAYLSQNPHFCKSALARFTQWDFIDLVNKHGITWHEKTLGQLF
                     CDDSAQQIVDMLVDECEKGNVTFRLRSEVLSVAKDETGFTLELNGMTVGCEKLVIATG
                     GLSMPGLGASPFGYKIAEQFGLNVLPTRAGLVPFTLHKPLLEELQVLAGVAVPSVITA
                     ENGTVFRENLLFTHRGLSGPAVLQISSYWQPGEFVSINLLPDVDLETFLNEQRNAHPN
                     QSLKNTLAVHLPKRLVERLQQLGQIPDVSLKQLNVRDQQALISTLTDWRVQPNGTEGY
                     RTAEVTLGGVDTNELSSRTMEARKVPGLYFIGEVMDVTGWLGGYNFQWAWSSAWACAQ
                     DLIAAKSS"
     gene            82844..84343
                     /gene="pitA"
                     /locus_tag="PN977_000378"
     CDS             82844..84343
                     /gene="pitA"
                     /locus_tag="PN977_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitA"
                     /protein_id="HDI6040459.1"
                     /translation="MLHLFAGLDLHTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMRSQLAVVMAAVFNFLGVLLGGLSVAYAIVHMLPTDLLLNMGSSHGLAMVFSMLL
                     AAIIWNLGTWYFGLPASSSHTLIGAIIGIGLTNALMTGTSVVDALNIPKVLSIFGSLI
                     VSPIVGLVFAGGLIFLLRRYWSGTKKRARIHLTPAEREKKDGKKKPPFWTRIALILSA
                     IGVAFSHGANDGQKGIGLVMLVLIGVAPAGFVVNMNATGYEITRTRDAINNVEAYFEQ
                     HPALLKQATSADQLVPAPEAGATQPAEFHCHPSNTINALNRLKGMLTTDVESYDKLSL
                     DQRSQMRRIMLCVSDTIDKVVKMPGVSADDQRLLKKLKSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVATTIGEKIGKKGMTYAQGMSAQMTAAVSIGLASYTGMPVSTTHVL
                     SSSVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAVLLSGGLYWLSLQFL"
     gene            complement(84414..84749)
                     /gene="uspB"
                     /locus_tag="PN977_000379"
     CDS             complement(84414..84749)
                     /gene="uspB"
                     /locus_tag="PN977_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspB"
                     /protein_id="HDI6040460.1"
                     /translation="MISTVALFWALCVVCIVNMARYFSSLRALLVVLRNCDPLLYQYV
                     DGGGFFTSHGQPNKQVRLVWYIYAQRYRDHHDDEFIRRCERVRRQFILTSALCGLVVV
                     SLIALMIWH"
     gene            85140..85574
                     /gene="uspA"
                     /locus_tag="PN977_000380"
     CDS             85140..85574
                     /gene="uspA"
                     /locus_tag="PN977_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspA"
                     /protein_id="HDI6040461.1"
                     /translation="MAYKHILIAVDLSPESKVLVEKAVSMARPYNAKVSLIHVDVNYS
                     DLYTGLIDVNLGDMQKRISEETHHALTELSTNAGYPITETLSGSGDLGQVLVDAIKKY
                     DMDLVVCGHHQDFWSKLMSSARQLINTVHVDMLIVPLRDEEE"
     gene            85890..87359
                     /gene="dtpB"
                     /locus_tag="PN977_000381"
     CDS             85890..87359
                     /gene="dtpB"
                     /locus_tag="PN977_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpB"
                     /protein_id="HDI6040462.1"
                     /translation="MNTTTPMGMLQQPRPFFMIFFVELWERFGYYGVQGVLAVFFVKQ
                     LGFSQEQAFVTFGAFAALVYGLISIGGYVGDHLLGTKRTIVLGALVLAIGYFMTGLSL
                     LKPDLIFIALGTIAVGNGLFKANPASLLSKCYPPKDPRLDGAFTLFYMSINIGSLIAL
                     SLAPVIADRFGYSVTYNLCGAGLIIALLVYIACRGMVKDIGSEPDFCPMSFSKLLYVL
                     LGSVVMIFVCAWLMHNVEVANLVLIVLSIVVTIIFFRQAFKLDKTGRNKMFVAFVLML
                     EAVVFYILYAQMPTSLNFFAINNVHHEILGFSINPVSFQALNPFWVVLASPILAGIYT
                     HLGNKGKDLSMPMKFTLGMFMCSLGFLTAAAAGMWFADAQGLTSPWFIVLVYLFQSLG
                     ELFISALGLAMIAALVPQHLMGFILGMWFLTQAAAFLLGGYVATFTAVPDNITDPLET
                     LPVYTNVFGKIGLVTLGVAVVMLLMVPWLKRMIATPESH"
     gene            complement(87408..88160)
                     /gene="rsmJ"
                     /locus_tag="PN977_000382"
     CDS             complement(87408..88160)
                     /gene="rsmJ"
                     /locus_tag="PN977_000382"
                     /EC_number="2.1.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1516)-N(2))-methyltransferase
                     RsmJ"
                     /protein_id="HDI6040463.1"
                     /translation="MKICLIDETGTGDGALSVLAARWGLEHDEDNLMALVLTPEHLEL
                     RKRDEPKLGGIFVDFVGGAMAHRRKFGGGRGEAVAKAVGIKGDYLPDVVDATAGLGRD
                     AFVLASVGCRVRMLERNPVVAALLDDGLARGYADAEIGGWLQERLQLIHASSLTALTD
                     ITPRPQVVYLDPMFPHKQKSALVKKEMRVFQSLVGPDLDADGLLEPARLLATKRVVVK
                     RPDYAPPLANVATPNAVVTKGHRFDIYAGTPV"
     gene            complement(88168..90210)
                     /gene="prlC"
                     /locus_tag="PN977_000383"
     CDS             complement(88168..90210)
                     /gene="prlC"
                     /locus_tag="PN977_000383"
                     /EC_number="3.4.24.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase A"
                     /protein_id="HDI6040464.1"
                     /translation="MTNPLLTPFELPPFSKILPEHVVPAVTKALNDCRENVERVVAQG
                     APYTWENLCQPLAEVDDVLGRIFSPVSHLNSVKNSPELREAYEQTLPLLSEYSTWVGQ
                     HEGLYKAYRDLRDGDHYATLNTAQKKAVDNALRDFELSGIGLPKEKQQRYGEIATRLS
                     ELGNQYSNNVLDATMGWTKLVTDEAELAGMPESALAAAKAQAEAKELEGYLLTLDIPS
                     YLPVMTYCDNQALREEMYRAYSTRASDQGPNAGKWDNSKVMEEILALRHELAQLLGFE
                     NYAFKSLATKMAENPQQVLDFLTDLAKRARPQGEKELAQLRAFAKAEFGVDELQPWDI
                     AYYSEKQKQHLYSISDEQLRPYFPENKAVNGLFEVVKRIYGITAKERKDVDVWHPDVR
                     FFELYDENNELRGSFYLDLYARENKRGGAWMDDCVGQMRKADGSLQKPVAYLTCNFNR
                     PVNGKPALFTHDEVITLFHEFGHGLHHMLTRIETAGVSGISGVPWDAVELPSQFMENW
                     CWEPEALAFISGHYETGEPLPKELLDKMLAAKNYQAALFILRQLEFGLFDFRLHAEFR
                     PDQGAKILETLAEIKKLVAVVPSPSWGRFPHAFSHIFAGGYAAGYYSYLWADVLAADA
                     FSRFEEEGIFNRETGQSFLDNILSRGGSEEPMDLFKRFRGREPQLDAMLEHYGIKG"
     gene            90413..91255
                     /gene="rlmJ"
                     /locus_tag="PN977_000384"
     CDS             90413..91255
                     /gene="rlmJ"
                     /locus_tag="PN977_000384"
                     /EC_number="2.1.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase"
                     /protein_id="HDI6040465.1"
                     /translation="MLSYRHSFHAGNHADVLKHTVQSLIIESLKEKDKPFLYLDTHAG
                     AGRYQLGSEHAERTGEYLEGIARIWQQDDLPAELEAYINVVKHFNRSGQLRYYPGSPL
                     IARQLLREQDSLQLTELHPSDYPLLRSEFQKDSRARVEKADGFQQLKAKLPPVSRRGL
                     ILIDPPYEMKTDYQAVVSGIAEGYKRFATGTYALWYPVVLRQQIKRMIHDLEATGIRK
                     ILQIELAVLPDSDRRGMTASGMIVINPPWKLEQQMNNVLPWLHSKLVPAGTGHATVSW
                     IVPE"
     gene            91327..92679
                     /gene="gorA"
                     /locus_tag="PN977_000385"
     CDS             91327..92679
                     /gene="gorA"
                     /locus_tag="PN977_000385"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HDI6040466.1"
                     /translation="MTKHYDYIAIGGGSGGIASINRAAMYGQKCALIEAKELGGTCVN
                     VGCVPKKVMWHAAQIREAIHMYGPDYGFDTTINKFNWETLIASRTAYIDRIHTSYENV
                     LGKNNVDVIKGFARFVDAKTIEVNGETITADHILIATGGRPSHPDIPGVEYGIDSDGF
                     FALPALPERVAVVGAGYIAVELAGVINGLGAKTHLFVRKHAPLRSFDPMISETLVEVM
                     NAEGPQLHTNAIPKAVVKNADGSLTLELEDGRSETVDCLIWAIGREPANDNINLEAAG
                     VKTNEKGYIVVDKYQNTNVEGIYAVGDNTGAVELTPVAVAAGRRLSERLFNNKPDEHL
                     DYSNIPTVVFSHPPIGTVGLTEPQAREQYGDDQVKVYKSSFTAMYTAVTTHRQPCRMK
                     LVCVGPEEKIVGIHGIGFGMDEMLQGFAVALKMGATKKDFDNTVAIHPTAAEEFVTMR
                     "
     gene            complement(92730..92816)
                     /locus_tag="PN977_000386"
     CDS             complement(92730..92816)
                     /locus_tag="PN977_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Damage inducible protein"
                     /protein_id="HDI6040467.1"
                     /translation="MIDKAIIVLGALIALLELIRFLLQLLNW"
     gene            93557..93910
                     /gene="arsR"
                     /locus_tag="PN977_000387"
     CDS             93557..93910
                     /gene="arsR"
                     /locus_tag="PN977_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="As(III)-sensing metalloregulatory
                     transcriptional repressor ArsR"
                     /protein_id="HDI6040468.1"
                     /translation="MSFLLPIQLFKILADETRLGIVLLLSELGELCVCDLCTALDQSQ
                     PKISRHLALLRESGLLLDRKQGKWVHYRLSPHIPAWAAKIIEQAWRCEQEKVQVIVRN
                     LARQNCSADSKNICS"
     gene            93965..>94884
                     /gene="arsB"
                     /locus_tag="PN977_000388"
     CDS             93965..>94884
                     /gene="arsB"
                     /locus_tag="PN977_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite/antimonite:H(+) antiporter ArsB"
                     /protein_id="HDI6040469.1"
                     /translation="MLLAGAIFVLTIVLVIWQPKGLGIGWSATLGAVLALVTGVVHPG
                     DIPVVWNIVWNATAAFIAVIIISLLLDESGFFEWAALHVSRWGNGRGRLLFTWIVLLG
                     AAVAALFANDGAALILTPIVIAMLLALGFNKGTTLAFVMAAGFIADTASLPLIVSNLV
                     NIVSADFFSLDFREYASVMVPVDIAAILATLVMLHLYFRKDIPQNYDMALLKSPAEAV
                     KDPATFKTGGVVLLLLLVGFFVLEPLGIPVSAIAAVGALILFVVAKRGHAINTGKVLR
                     GAPWQIVIFSLGMYLVVYGLRNAGLTEYLSG"
BASE COUNT        22817 a        25437 c        23673 g        22957 t
ORIGIN      
        1 cgaaaaagtg gcgatcaaat cccgcgcacc gatcctgctc aacggcccaa ccggtgcggg
       61 aaagtcgttt ctggcacgac gcatcttcga gttaaaacag gcgcggcatc agtttagcgg
      121 cgcgtttgtg gaggtgaact gtgccactct gcgcggcgat accgccatgt cagcgctgtt
      181 tggtcatgtg aaaggcgcgt ttaccggggc gcgggagtcg cgggaaggat tattacgcag
      241 cgccaacggc gggatgttat ttcttgatga gattggcgaa ctgggcgcag acgaacaggc
      301 aatgctgctg aaagccattg aagagaaaac cttttatccg tttggcagcg atcgccaggt
      361 gagtagcgac tttcagctga tcgccggaac ggtgcgcgat ttgcgtcagc tggttgccga
      421 aggcaaattc cgcgaagatc tgtacgtgcg gatcaatctc tggaccttca ccctgccggg
      481 gctacgccag cgccaggaag atattgaacc taacctggat tatgaagtgg agcgccacgc
      541 ctcacttacc ggcgacagcg tgcgttttaa caccgaagcg cggcgcgcct ggctggcctt
      601 tgcaacatca tctcaggcgg catggcgcgg taactttcgc gagctttctg ccagcgtcac
      661 gcgaatggca acctttgcca ctagcggacg catcactctg gaagtggttg gagatgagat
      721 aaaccgtctg cgctataact ggcaggagag tcgtccttcc gcgcttacgg cgttgctggg
      781 cgctgaggca gaaaacatcg acctctttga ccgtatgcaa ctggaacatg ttatagctat
      841 ctgccggcag gcaaagtcgc tttccgctgc cggacgccag ctttttgacg tttcgcgcca
      901 ggggaaagcc agcgtcaacg acgcggatcg gctacgcaaa tacctggcgc gttttaatct
      961 gacgtgggaa gccgtgcagg atcagcacag ctccagttga atatggtggt ccgtcagcac
     1021 ctgcatcacg cttactggcg gcggggcgtc ggtgtagacg gcatctacca tgctgatact
     1081 gcccatattg accatcgcgt tacggccaaa ttttgagtgg tcgacaacca gcataacgtg
     1141 gcgcgagttc tcaatgatgg cgcgtttggt gcgaacttcg tggtaatcga actccagtag
     1201 cgagccgtcg ctatcgatgc cgcttatccc cagaatgccg aaatcaaggc ggaactggga
     1261 gataaaatcg agcgttgctt cgccaatgat cccgccatcg cggctgcgta attcgccacc
     1321 ggcaagaatg atacggaaat cctcttttac catcagcgtg ttagcgacgt tgagattgtt
     1381 ggtcacaatg cgtaaattgc tgtgattaag cagcgcgtgc gccaccgctt ccggcgtggt
     1441 gccgatatcg ataaacagcg tcgagccatt gggaatttgc tccgccactt tgcgggcgat
     1501 gcgctctttt tcttcggtct gggtggcctt gcgatcgtgc cacggcgtat taaccgaact
     1561 ggaaggcagc gccgcaccgc catgatggcg caggatcagg ttttgctctg ccagctcgtt
     1621 gagatcgcgg cgaatagtct gcgggctaac ggagaaatgc tctaccagct cctcggtgct
     1681 gacataaccc tgctgtttaa ccagttcgat aataccgttg tgacgttgtg tttgtttcat
     1741 ttctaaatcc ctggaattat tttcgttttc gcgcattgag cgaatcaaca aaagccatcg
     1801 ctaaacccac ggctaacccg gcgatgtgtg ctccgttcgc catcgacatc ccaaacaaat
     1861 caaaccatcc ggcgacaatc cagatcaacg caaagataat taacccacgt tgcaggtaaa
     1921 tgccactttg cggatcgcgt tcgccacgta gccagacgta gcccatcagc gcatacacca
     1981 cgccagaaag cccgccaaac cacggcccgc tgaatttttg ctgcacatag ccgcttaaca
     2041 gggcgctaat gagagtaatg acaattagct taccgctacc gaggcgtttt tccaccgcac
     2101 cgccgagata ccaccaccag agcaggttaa agaggatatg catcagcgag aagtgcatta
     2161 acgcgtgggt gaagtaacgc cagaactcaa atttcagcgt cggatcgaat ggccaggcca
     2221 gccataacat cacttcctga tcgccgagaa tttgcatggc gataaacacc accacgcagg
     2281 caatcatcat cacccaggtt accggacctg cgcgttcacg caaggcagca aagaaaggat
     2341 aacggcgata atgcaggcca ctgccggtat ggcccgcctg ccagctggcc gccagataac
     2401 gcggatctgc cgggttttcg agaaaacgcg ccagctccgc ccgcacgcgt tcggcctggg
     2461 actcatccgc tagccagaca tcgctttggt tatgttgttg aatcgtgagg ataacaccct
     2521 gcgtcgccat gtaatcaaca aacgcctgcg ccactcgggg gttagcaaaa gaggtaatca
     2581 tcaacatcgt tgctgtcgct tattccacac aaaaggggac agtataaagc gttacgcgcc
     2641 gtacgccacc tctgcgggaa actggcgctg ccaggcttca aagccgccat caatgctgta
     2701 gaccacatcg tagccctgtt gcagcagata ctgcgccgcg cctttgctgc tattaccgtg
     2761 gtaacacatc accatgactg gcgtatcgaa gtcgttatca cgcataaacg cgcccaatgt
     2821 gtcgttggtt aaatggaaag cctgcgccgc atgccccatt gcgaaactct gtggatcgcg
     2881 aatatcgacc agcaccgcct ctttttcctg caacttctgg tgcgcgtcgg caacgttaat
     2941 acattcgaac tgatccatgc gtctctcttt ctttacaaac aagtgggcaa atttaccgca
     3001 cagtttacgt cgaagcggca gataaacgcc ataatgttat acatatcact ctaaaatgtt
     3061 ttttcaatgt tacccaaagc gcgattcttt gctaatatgt tcgataacga acatttatga
     3121 actttaacga aagtgaatga gggcagcatg gaaaccaaag atctgattgt gataggtggc
     3181 ggcatcaatg gtgctggtat cgcggcagac gccgctggac gcggtttatc cgtgctgatg
     3241 ctggaggcgc aggatctcgc ttgcgcgacc tcttccgcca gttcaaaact cattcacggt
     3301 ggcctgcgct accttgagca ctatgaattc cgcctggtca gcgaggcgct ggctgaacgt
     3361 gaagtgttgc tgaaaatggc tccgcatatc gccttcccga tgcgttttcg cctgccacat
     3421 cgtccgcatc tgcgcccggc gtggatgatt cgcattggtc tgtttatgta cgatcatctg
     3481 ggtaaacgca ccagcctgcc gggatcaacc ggtttgcgtt ttggcgcaaa ctcagtgtta
     3541 aaaccggaaa ttaagcgcgg attcgaatat tctgactgtt gggtagacga cgcccgtctg
     3601 gtactcgcca acgcccagat ggtggtacgt aaaggcggtg aagtgcttac tcgtactcgt
     3661 gccacttctg ctcgccgcga aaacggcctg tggattgtgg aagcagaaga tatcgatacc
     3721 ggcaaaaaat acacctggca ggcgcgcggc ctggtgaacg ccaccggccc gtgggtgaaa
     3781 caatttttcg acgacgggat gcatctgcct tcaccttatg gcattcgcct gatcaaaggc
     3841 agccatattg tggtgccacg cgtgcatacc cagaagcaag cctacattct gcaaaacgaa
     3901 gataaacgta ttgtgttcgt gatcccgtgg atggacgagt tttccatcat cggcaccacc
     3961 gatgtggaat acaaaggtga tccgaaagcc gtgaagattg aagagagtga aatcaattac
     4021 ctgctgaaag tgtataacac gcactttaaa aagcagctaa gccgtgacga tatcgtctgg
     4081 acttactccg gcgtgcgtcc gctgtgtgat gatgagtccg actcgccgca ggcaattacc
     4141 cgtgattaca cccttgatat ccatgacgaa aacggcaaag caccgctgct gtcggtattc
     4201 ggcggtaagc tgaccaccta ccgcaaactg gcggaacatg cgctggaaaa actaacgccg
     4261 tattatcagg gtattggccc ggcgtggacg aaagagagcg tgctgccggg tggtgccatt
     4321 gaaggcgatc gcgacgatta tgccgctcgc ctgcgccgcc gttacccgtt cctgactgaa
     4381 tcgctggcac gtcactacgc gcgcacttac ggcagcaaca gcgaactgct gctcggcaat
     4441 gcgggggcga taagcgacct cggggaggat ttcggtcatg agttctacga agcggaactg
     4501 aaatacctgg tggatcacga atgggtccgt cgcgccgacg atgccctgtg gcgtcgcaca
     4561 aaacaaggca tgtggctaaa tgcggatcaa caatctcgtg tgagtcagtg gctggtggag
     4621 tatacgcagc agaagttgtc gctggcgtcg taaattaacg taaggtggcc aggtcagatt
     4681 tcaatctgac ctgagactga tgataaacaa aaaaactgcc tgatgcgcta cgcttatcag
     4741 gcctacgtgg tttatgcaat atattgaatt tgcatgattt tgtagacaaa ataaggcgtt
     4801 cacgcagcat ccggcataaa caaagcgcac ttcgtcaaca atctgaccta ctttttgatt
     4861 caaggaatta ccgcaaagcc cacgcaatca tacccaacgt aacaaaactc agacacatta
     4921 cccactcgcc ccacttcttc ctctgacttc tatttttgct acgccactgg caataatatc
     4981 ccaatgcaaa accaagctgg gcgcttatcg gaccgataaa gtcagtccac ggctgagacc
     5041 caaattgaaa aaattcaggg gtaaccagac aatggtcaga aagaaattgc gcatattttc
     5101 cagcaaagca atttgcgctg gcgcgacata gaaaatgata ttccacagga gcaaccagaa
     5161 ataaactggt gcaaatacca accaacaatg gagtctgctt ttcagttcag attgagctct
     5221 gcaagaaaat tgccaaccaa cgcaacacat ggcacagaaa aaaaaagtgc atattcagcc
     5281 aataacccaa gttggaattc cagaagtgaa atacgcctga acatgagcgg gatagagaac
     5341 agtatttctc ccataaaact cagcgtgata acacaagcag ccagagcaac caccgtcggc
     5401 cacaccaaca acggcacaat aacaaaacca agaattaacg gaggaatata aagtctgatt
     5461 ttattcatct ttattgtcct gatgattcgg atgatgcgtc ataaatcggc acatagccca
     5521 catcctctac caattgctgt ccctgcgggc ttaaaaacca gtcgacgaat ttctgcgttt
     5581 ccagcgtagg gttttcgtgc gtcaccatat aagcatgaat tatgtacgga taagtgccgt
     5641 tacggatatt ttcctctgta ggtgcaacgc catttatagc cagcagtttg atctgtttgc
     5701 gcattttgag catattgtca ttcatacgtg tgacgtaata gtggaaggtg tatccaatag
     5761 aagatggtgt attttggtag tcggcgactt cggtaattaa accgcccata agatcgataa
     5821 cagtggattt tttggctggc agcattggag tatcttgcat aaccagtccc ttcataaccg
     5881 tttggcttcc agaatcttct ggccgctgcc agacttttat actttcgtca ccgccgccca
     5941 ctttattcca gcgactcact ttaccgctaa aaatgtcttt gacctgctgt tcgctaagtg
     6001 aattaaccgg gtttttgata tcaacgataa agacaaaggc ttcgcgggcg aaagcggtat
     6061 atttgagttt aacacctgct tcttctgcac gttttttctg tccttccgac ggttgtgcaa
     6121 caaaaataac cgtcgcggta cccttaatca gactatcgta ggcttcagct gttcgggttt
     6181 gttgcaaacc atacgcctgc caggccagcg aggttcgatc cttatctttt ggctcgggta
     6241 ctaattcata atacgcggag gcatacagcg ggtataaggc tgtagcgccg tacactctcg
     6301 gccatcttga ccaatcatcg ttgtaacgta attcaggttg cccccgcaga gacgtcagtc
     6361 catggctgtc ttcttccctg acgctgttat agtacccttc ccggtcgaca catcccgcca
     6421 gtagcgccgt acctaatagt aaaacaccgg tgaatttatt cattgcgctg attcctttct
     6481 ctcgagcgtt atttcattgt tggatacagc ggcacatagc ccacatcttc caccagactc
     6541 tgtccctgcg gcgttaaaaa ccattcgacc agtttttgtg tttctgacgt agtattttcc
     6601 cgcgttacca taaaggcatc gatgatatac ggatatttgc cgttacgaat attttccgca
     6661 gtcggtgcaa taccgttaat cgctagcagt tttatatttt tatcggcatt catttgtgtt
     6721 gcgtaatagc ggaaggtgta gcctattgcg ttgtttgtat tacggtattc ggcaacgact
     6781 ttaatcattc cctccatcat gcttgccact tcggtttcct gcggcgagat catgcggaca
     6841 tttttcatga cctgcgattg catcactgtc tggctgccag agtcttccgg gcgttgccag
     6901 gtctggatct cctgatcgtt accgccaacc gtgcgccagt tggtaattgc gccactaaaa
     6961 atgtcacgca cttgttgttc agtcagggaa ttaaccggat tatccgcatt gacgatgaaa
     7021 acaaacgctt cacgggcaaa tggcgtgtac agcaaagtga cgcccgattt ctcagcgcgt
     7081 tttttttgcc catcggaagg ttgcgccacg aaaataatat cagcatcgcc cttaacgatt
     7141 ttgttatatg cttcttgcgt acgagagtta tccagataat cccaaacgtg aaaatcctct
     7201 ggtattacac ttaatgcata aaatgcagag gcataaatgg ggtacgccgc cgtagcgcca
     7261 tcgagtcgcg gccagttttg cgtaaattga atttgtggtt taccacgcag tggcgtcagt
     7321 tgattattaa gtttatcggg tcgccaggcc caggtatcaa tattttcacg cacaccgacg
     7381 ccgggattaa gctgtttata taaataagcc tgataaccag cgacaatacc cagtaagaga
     7441 agaatgaaca gaagtaaatt acggcaacgt agaccggagg tgccggttaa agggtgtctg
     7501 gtcggccagc cgtatcccag tgaaaaactg atatgcgcgc caacgggaat aatgactatc
     7561 caccagtact caccaccgac gaatgagact gtaaaaacgc ccaaccaggg aagcataagg
     7621 tttgccaacc aacctgaaac catctggcga aattgataat ctgccagatg tagcgtgatt
     7681 atccagccaa ccagggttaa taaagcggga actaaaaaaa gacattgtcg ccaggcggct
     7741 atttctctgt tttctggtgc gctatcgccc gcccacactc gcccagagaa aaatagcagt
     7801 aatgcgccca gcgtcagcat aaagacgttg agtagaagat aaattgttgg cgtaaagagt
     7861 aaattgcaaa catcaaaaat agcggcaagc ccggccccca gcatggcaac aaccgctgcc
     7921 aaaaattgcg ccagtatggg gatgccaaaa aaagtcatta ccagcgaggt caaaatccat
     7981 tttccgtttt gcattattct ttccattctt gctgaatggc gaaattatac tccccgataa
     8041 ccctcgcccc ctctggacac ttttcctaaa taaaggcgaa aaaaaacggg accttttggc
     8101 cccgttctgt tttttagcga acttacaatc tcaccggatc aatatgccag atatgatcgg
     8161 cgtactcttt gatcgtacgg tcagaagaga agtagcccat attggcaatg ttcagcatcg
     8221 cttttgcggt ccactcttcc tgacgctcgt agagttcgtc gactttatcc tgacaatcga
     8281 catagctgcg ataatccgcc agtacctggt agtgatcgcc gaagttgatc agcgaatcga
     8341 ccagatcgcg atagcgaccc ggatcttccg gactgaatac accgctgccg atttgcgtca
     8401 gcacctgatg cagctcctca tctttctcgt agtattcacg cggtttgtag ccctgacgac
     8461 gcagttcttc cacttcttcc gctgtgttac caaagataaa gatattgtca gcaccgacat
     8521 gatccagcat ctcgacattc gcaccgtcca gcgtaccgat agtcagcgcg ccgttaagcg
     8581 caaacttcat gttactggtg ccggaagctt ccgtccccgc cagcgaaatc tgttcagata
     8641 gatcggccgc cggaatgatc aactgcgcca ggctaacact gtagttcggg atgaacacga
     8701 ctttcagctt gtcgccaatc tgcggatcgt tgttgatcac tttcgctacg tcattgatca
     8761 aatgaataat gtgcttcgcc atgtaatagg ccgaagccgc cttaccgcca aaaatattca
     8821 cgcgcggtac ccacttcgca tccgggtcgg ccttgatgcg gttatagcgg gtgatcacgt
     8881 gcaacacatt catcaattga cgtttgtatt cgtgaatacg tttgatttgc acatcgaaca
     8941 gcgctttcgg atttaccacc acattcagct gctgggcgat atactccgcc agacgctttt
     9001 tgttctccag cttcgcctga tgcaccgcgt gattaaccat tgggaaatca cagtgttgtt
     9061 gcagctcatt aagcagacta aggtcggtgc gccagttgcg gcccaggtgt tcgtccaaca
     9121 cagctgaaag cgatgggttc gctaccgcca gccagcgacg cggcgtcaca ccgttggtga
     9181 cgttggtgaa acgacccggg aagattttcg caaagtcggc aaatagcgat tgcaccatca
     9241 ggttagagtg cagctccgat acaccgttaa ctttgtggct caccacaacc gccagccagg
     9301 ccatacgcac acgacgaccg ttggattcat cgatgatcga cgcccgtccc agcagatcgg
     9361 tatcgttcgg atactgttcc tgcaaagttt tcaggaaata gtcgttgatt tcaaagatga
     9421 tctgcaggtg acgcggcaga attttaccca gcatatcgac tggccaggtt tccagcgcct
     9481 cgctcattag cgtgtggttg gtgtaggaga agacctgaca acacacttca aacgcgtcgt
     9541 cccagctaaa ttggtgctca tcgatcagca gacgcatcat ctcaggaatc gacagcaccg
     9601 gatgggtatc gttgaggtga atcgcaattt tatccgccag gttatcgtag gttttatgca
     9661 actgataatg gcggcttaaa atgtcctgaa tggtcgagga aaccaggaag tattcctgac
     9721 gcagtcgcag ctcacgcccg gagtaggtgg agtcgtccgg atacagtacg cgagatacgt
     9781 tctcggagtg gtttttatct tccactgccg cgaagtagtc gccctggttg aatttaccga
     9841 ggttaatttc gctactggct tgggcactcc acaaacgcaa cgtgttggtc gcgtcggtgt
     9901 cgtaacctgg gattatctga tcgtaagcta ctcccagaat ctcttcggtt tcaatccagc
     9961 gcgttttttt accttcctgc tgaatgcgac cgccaaaacg gactttatag cgcgtgttgt
    10021 ggcgtttgaa ttcccacggg ttaccgtatt ccagccagta gtcaggcgac tctttctggc
    10081 taccgttaac gatgttctgc ttgaacatac cgtagtcata gcggatgccg taaccgcgcc
    10141 ccggcaaccc taacgtcgcc agagaatcga ggaagcaagc cgccagacgt cccaggccac
    10201 cgttaccgag gcccgggtca ttttcttcat caatcagctc ttcgagattt aaccccatcg
    10261 cttccagtgc gccctgtaca tcttcgtaaa ttcctagcga caacatggcg ttggagagcg
    10321 tacggccaat caaaaactcc atcgacaggt agtaaacctg acgagtttct tgcgacaact
    10381 gggcacggtt tgaacgtaac cagcgctcca cgagacgatc gcgcacagca aataatgttg
    10441 cgttcagcca ttcatgttta ttggcgacga ccgggtcctt gccaatcgta aacatcagct
    10501 tgtaagcgat agaatgctta agagcttcta cgctaagcgt gggcgatgaa tatgtaaacg
    10561 gagcattcat ataggcgttt cctgaaaact atttcaagcg atagtaaagc tcacggtacg
    10621 acttcgccgc gacctgccag ctaaaatcca ttgccatagc ctgacgttgc acaaaccgcc
    10681 acagtgaagg acgggaccac agtacaaaag cacgtcgaat agcccgtaat agcgaccagg
    10741 cattactatc ttcaaagaca aacccactgg cgacgccatc tgcaaggttc tcaagagaac
    10801 agtcagaaac cgtatcagca agcccaccgg tgcgccgcac taacggcagc gtaccgtact
    10861 tcaatccata aagttgcgtt aagccgcacg gttcaaaacg gctgggcacc agaatgacgt
    10921 ccgcgccgcc cataatgcga tgcgaaaatg cttcgtgata gccaatctga acgcccacct
    10981 ggccggggta ttccgctgcc gccgcaagga aaccttcctg cagcaccgga tcgcccgcgc
    11041 cgagtagcgc cagttgcccg ccctgctcca gaagacccgg taaggcctcc agcaccaggt
    11101 cgagaccttt ctggctggtc agacggctca ccactgcaaa aagcggcact ttatcgtcaa
    11161 ccttaagccc cattgcgatt tgtaactggc gcttattttc cgctttatct tccaacgtat
    11221 cgcgggtgta acgcgaggcc aacagtaagt ccgtctctgg actccagatt ttctcgtcca
    11281 cgccgttcag tacgccggaa agacgccctt cacggtgacg ctgttgcaac agaccttcca
    11341 taccgtaggc aaactgcggt tcggtgatct cgcgagcgta ggttggactg actgccgtaa
    11401 tgtgatcggc atagtacaga ccggccttca gaaaagagat ttgtccgttg aattccagcc
    11461 catgaatatt aaagaatgac catggcaatt ggatgtcatt catgtgatgt gcataaaaca
    11521 tgccttgata ggccaggttg tgcacagtaa acaccgactt cgccggacgc ccgcgcgccg
    11581 ccagatacgc aggcgcaagg cctgcatgcc agtcgtgcgc atgcaccaca tcaggacgcc
    11641 agaatgggtc aagcccactg gccatttctg ccccaaccca ccccagcagc gcaaaacgca
    11701 atacgttgtc ggtataggca aataagttgg tatcgtgata cgggcttccc ggacgatcat
    11761 agagatgcgg cgcgtcaatc aggtatatgc caaccccgtt gtaatgaccg aacaacagcg
    11821 tgatatgtcc ggcgaaggta tcacgacggg atactacctg cgcatcggtc acgccacggc
    11881 gaatatcggg aaatgcaggc aacagtacgc gagcgtcaac gccgtctgcg atttgtgctg
    11941 cgggtaatgc cccaataaca tcagccagac cgccggtttt aagcagcggg aacatctctg
    12001 aacatacatg taaaacctgc attatcgctc ctgtttatgc cctaacttcc gtagcatttc
    12061 gcgcgttacc agcacgatgc cttcttctga acgatagaaa cgacgtgcat cttcctctgc
    12121 gttttcacca atcaccatgc cttccggaat aacacaagca cgatcgatga cgcagcggcg
    12181 cagacggcac gagcgaccta cccatacttc cggtaacaat acggcggaat caatgttgca
    12241 gaatgaattc acgcgaacgc gcgagaacag aacggactgc accaccaccg aaccggagat
    12301 cacacaaccg ccggatacca gtgagttaag ggtcatcccg tggctaccgg agcgatcctg
    12361 cacgaatttc gctggcggta atgattcatt gtaggtgcga attggccaat tgcgatcgta
    12421 catatccagt tccggcacca cagaggccag atcgaggttc gctttccagt aagcttccag
    12481 cgtacccaca tcgcgccagt acggctcggc atccgggtcg gattgtacgc aagagagcgg
    12541 gaacggatgc gcataggcca gaccggcttc ggtgatcttg ggaatcaaat ctttgccaaa
    12601 gtcgtggctg gagttctcat cgcgatcgtc ttcttccagc agttcataca gatagtcggc
    12661 gtcaaagacg taaataccca tactcgccag agatttgctc ggatcgttcg gcattgacgg
    12721 cgggttagct ggtttttcaa cgaattcgat aattttatcg ttctcatcaa ccgccataac
    12781 gccaaacgcg gaggcttctt caatcggtac tggcatacaa gcaacggtgc aacgtgcgcc
    12841 tttttcgacg tggtcgataa gcatacgcga gtagtcttgc ttgtagatat ggtcgcccgc
    12901 caggatcacc acgtattccg ctttataacg gcggataatg tcgaggtttt gggtgaccgc
    12961 atctgcggtg ccgcgatacc agttttcccc tttcattctc tgctgtgctg gcagcagatc
    13021 gacaaactcg ttcatttctt cattgaagaa tgaccagccg cgctgaatgt gctgcaccag
    13081 agtgtgggac tggtactggg tgatcacgcc catacgacgg atcccggagt tgatgcagtt
    13141 agacagcgca aagtcgataa tgcggaactt accgccgaag tgtacggccg gttttgctcg
    13201 cttattggtt aaatccttca ggcgggtacc acgtcctccc gccagtatca gggcaacaga
    13261 tttcaatggc agctggcgcg ccaacattaa gtgatcgttc ttctctaaac taaccatgac
    13321 taactccttt tttatcatct ctggaacaca cacaatccgt gtgcaggtcc ctgccagaca
    13381 gccgtaatca ctgggttatc ctctccagcg aatgggggaa tggcgtgcca ctccccagca
    13441 ggtaaaacaa tctctgttac ctcaagcgtg gcgttaattg cgatcaaaaa gcgatccgag
    13501 agcagaattt gcagctgttt cggcccgttt tgccactcat ccgtgcttaa aggttgagca
    13561 tatcgattta gccaacggac attgccgtcg ccttcttccc accagcgatt ctccaccaaa
    13621 gcgggaatgc gcttgcgcag atggattaac gcggcggtaa atgcggttaa accactgctt
    13681 gcctgcgacc agtccaacca ggttaattgg ttatcctgac agtaggcatt gttattgcca
    13741 tgctggctgt gaccatgttc gtcaccggcc agtaacatcg gcgtaccctg ggagagcaac
    13801 aacgttgtta acagggcgtg aatgctgtcg cgacgccgtt caaccaggtc aagagtaccg
    13861 cctaaccctt ctttaccatg attgttactg taattgttgt tggtcccgtc gcgattttct
    13921 tctccgtttg cttcattgtg tttatggttg aagcaaacgc agtcgcgaag cgtaaaaccg
    13981 tcatgcgcgg tgacgagatt aatcgcggca ctcggcagac gatcattacg tttaaaaaca
    14041 tcgctggagg cagcaaaacg cccggcaaac gcccccagag gcaaatcata atgaagccag
    14101 aaacgacggg cagcatcgcg gaaatgatcg ttccactcgg caaacagcgg cgggaaattt
    14161 cccacctgat aaccaccagg agcaatatcc cacggttcag caattaactt cacctgcgag
    14221 agcaccgggc agttctggat agcggtaaac aacggcgcat cctgacggaa ctctggcgta
    14281 cggcccatga ctgccgccag atcaaagcgg aaaccatcga cgtggcaggt ttctacccaa
    14341 taacgcaggc aagcgctggc ataatccact accgccggat gactcaaatt gagcgtgttg
    14401 ccacaaccgg tccagttgtg ataatcgccg tcttctctta tccaataata gctacggttg
    14461 tcgatcccac gcagagagaa taacggaccg tcgaggtcca gttccgcact atggttgagc
    14521 acgatatcaa gaatgacttc gatacccgct ttatgcagtg ctttgattgc atcgcgaaac
    14581 tcatccagcg ccgtttctgg ggagcaggca tacgccggat gcagcgcaaa catcgccacc
    14641 gggttataac cccagtagtt acttagaccc atacgttgca ggcgtggttc gctggcaaac
    14701 tgcgccaccg gcagcagttc cagcgcggta atgcccaact gtttcaaata gttgatcatc
    14761 accggatgcc ccagggcttt ataagtgcca cggatctcga ccgggatctc cgggtgcagg
    14821 tacgttaatc ctttgacatg ggcttcataa atgatggtgc tgccccacgg cgtgcgcggc
    14881 ggggcatcat cctcccagtc atagtgatca accaccacta cgcatttcgg cgcaatggcg
    14941 gcgttgtcgc gatagtcagg ttcattatga ccggcgtgta gcagcgggtt atctttaaac
    15001 tccccgtcaa tttgccgcgc gcaaggatca atcaacaact tcgccgggtt aaagcgatgc
    15061 ccctcggcgg gttgccaggg gccatgaacg cgataaccat aacgcaaacc cggacgcgca
    15121 tccggcagat aaccgtgcca aatgtcgcca ctgtgccctg gcaagtcata gcgatgttcc
    15181 tggccattgg cgtcaaagac acacagctct acccgctcgg catgagcgga gaaaagtgtg
    15241 aaattgacgc cctgaccgtc gtaatgcgcg ccgaggggag tgggtttgcc aatggcgagt
    15301 tgtgtcattc tgcctcccga accagccaga tagtggccag aggtggtagc gtcaggctta
    15361 gtgaatgctg acgaccgtgg ctggcaatct catcgctgtg taccgtgccg ccattgcctg
    15421 cattactgcc gtgatagtgc atggaatcgg tattgaggat ctcacgccat ttacccggct
    15481 ggttaatgcc gaagcgataa tcatgacgcg gcaccggagt aaagttactg gcaacgatga
    15541 tttcgttacc ctctttatcg cgacgcacaa agatcagcac cgagcgttct ttgtcatcca
    15601 ccaccagcca ttcaaagccg tacgggtcaa aatccagttc atgcattgct ttatggtggc
    15661 ggtaggtgag gttcagatcg cgcaccagac gctggacacc gtggtgccag ttatcgccgc
    15721 cttccaacag atgccagtcg aggctggcgt catggttcca ctcgcggccc tgggcaaatt
    15781 cgttccccat gaacagcaat ttcttgcccg ggaatgccca catccagccg tagtaggcgc
    15841 gcaggttagc gaatttctgc catgcgtcgc ccggcatccg gtcgagaatc gattttttac
    15901 cgtggaccac ttcatcatgc gacaacggca ggacgaagtt ttcagtgtag ttgtagagca
    15961 tcccgaaggt cagtttatcg tgatgatact gacgataaac tgggtcgagc ttcatgtagt
    16021 ccagggtgtc atgcatccag ccgaggttcc acttgtacca gaagcccaga ccgcccatgt
    16081 cctgcggacg agaaacgcca gggaaatcgg tagactcctc cgccattgtc actgcaccgg
    16141 aaacctgctc accaagaata cggttggtat tacgcaagaa ttcaatcgct tcaagattct
    16201 cgcgaccgcc aaattcgttc gggatccact ccccctcttt acggctgtag tcgcgataaa
    16261 tcattgacgc caccgcatcg acgcgcagcg catcaatacc aaaacgttcg atccagtaaa
    16321 gcgcgttacc gacaaggaag ttactgactt cacggcgacc atagttgtag atcagcgtgt
    16381 tccagtcctg atgatagcct tcgcgcggat cgctgtgttc atacaagttc gtgccatcaa
    16441 attcggcaag cgcaaagtca tcggtcggga agtggcctgg cacccagtcg agaatcacgt
    16501 tcagaccagc tgcgtgtgcg gcatcaatga aataacggaa gtcgtcgcgg gtaccaaaac
    16561 ggcgggtcgg tgcatacagg ccggttggct gataacccca actgccatcg aacggatgct
    16621 cgttaatggg cagtagctcg agatgggtaa agcccatcca tttagcataa ggcacaagtt
    16681 gatcggccag ctcgcggtag cttaaccaga aattgttgtc ggtgtggcga cgccaggagc
    16741 ccaggtgaac ttcataaata gagattggcg catcaaactg attcgctttt ttgcgctctt
    16801 cagtctgtac aacctttttc ggcagcccgc aaataagaga cgcggtttcc gggcgcattt
    16861 gcgcttcgaa ggcataaggg tcggacttca gacgcaagtt gccattggca tcaatcatct
    16921 cgtatttata gagctgaccg ttatgcgccc cagggataaa cagttcccag atgccgctct
    16981 ctttacgcag gcgcatcgga tgacggcgac cgtcccagta gttgaattgc ccaaccaccg
    17041 agacccgacg ggcgtttgga gcccagacag aaaaacgcgt acctgtgacg ccatccatag
    17101 tatctgcatg cgcgcctaag gtttcatacg ggcgcaggtg agtaccttca gataatagcc
    17161 aggcatccat ttcctggatt agcggaccaa aacggtaagg atcatctatc aggttttgct
    17221 gaccatgcca gacaacagcc aactgatagc ggaaaaaatt cttacgtcgc ggaatgacac
    17281 cgctaaagaa tccacgggag tcgagacact ccagttttgc gagtttgcgc ccggttttcg
    17341 gttcaatcac ccacacatcg gtagcgtcgg gtaaaagggc acggacttcc agtcccgcgg
    17401 tggttttatg catccccagt acggaaaaag gatccgcaaa atggcctgca attagcgcgt
    17461 taatcacgtc tctatcgata cgatcggaca tgcttgtctt cctgttttat tgtgtcaccc
    17521 catccaactg aatttttgac ttctggttgt gacatttttt tgacctgaac ggcgcagcac
    17581 tctgtgcatc ctctctgcgt cgtcctcact tcaggtaagg ctgtgaatac tcatgtattc
    17641 agccaccctt aaagaatagc caatgctcta tttaactccc ggtaaatcat gaaacatctg
    17701 cgcttactcc tgtattacgc actaacaggg gcggcatcgc gccccagatt taatgaataa
    17761 agattacgcc agttgacgaa gcatccgacg tagcggctcc gcggcccccc acagcagctg
    17821 gtcgcccacg gtaaaggctg acaggaactc cggtcccata ttcagcttac gcagacggcc
    17881 taccggcgtg gtcagcgtgc cggtaacggc agctggggtt agctcacgca tagtgatttc
    17941 ccgatcgttt ggaacaactt tcgcccacgg attgtgcgca gccagcagtt cttccacggt
    18001 cggaatagac acatcttttt tcaatttaat agtgaatgcc tggctgtggc agcgcaatgc
    18061 cccgacacgc acacataaac catctaccgg aattacggaa gatgtgttga ggatcttgtt
    18121 ggtttccgcc tgccctttcc actcttcgcg gctctgaccg ttatcaagct gtttgtcgat
    18181 ccacggaatc aggctacccg ccagcggcac gccaaagtta tccaccggca gctcaccgct
    18241 acgggttaag gttgtgactt tgcgttcgat atcgagaata gcagaggacg gggtcgcgag
    18301 ttcatctgcc acatggccat acagatggcc catctgggtt aataactcac gcatatgtcg
    18361 cgcaccaccg ccggaagcgg cctggtaggt tgcaacggac acccaatcaa caagatcatt
    18421 ggcgaataaa ccacccaacg acatcaacat caggcttacg gtacagttac cgccaacaaa
    18481 agtcctgatg ccattattta atccgtcggt aatgacgtcc tgattgacgg ggtcaagaat
    18541 gatgatggcg tcatctttca tgcgcagaga tgatgctgcg tcaatccagt aaccttgcca
    18601 tccgctttca cgaagctttg gatagatttc gttggtataa tcgccgccct gacaggtcac
    18661 aatgatatcg agggccttta gcgcctccag atcaaaggca tcctgaagtg tgtcagtggt
    18721 tccgccaaaa gacggcgcag cctgaccaag ctgagaagta gaaaagaaga cagggcgaat
    18781 ggcgtcgaag tcgcgctctt caaccatgcg ttgcatgaga acggagccga ccataccgcg
    18841 ccagccgata aaaccaacat ttttcataag cgttttttcc tgcaaagatg tgtgctgtat
    18901 aaatgtgccg gtctcctctt ggcacatctt tcaccataca aaaagcagcc aaagtcgcaa
    18961 gtgaaattaa tcaatgatag cgaagccatc agtaatgcga cttatcctgc tttgttagca
    19021 cgcagaaagt ccgcggcaat tatcagggaa tttgagttat gaatgaaatc atttctgcag
    19081 cagttttatt gatcctgatt atggatccgc tcggaaacct acctattttc atgtccgtac
    19141 tgaaacatac tgaaccgaaa agacggcggg caatcatggt gcgagagttg cttattgctc
    19201 tcctggtgat gctggtgttc ctgtttgcgg gtgagaaaat tctggcattt ctcagcctgc
    19261 gagcagaaac cgtctccatt tctggcggca tcattctgtt tctgatcgcc attaagatga
    19321 ttttccccag cgcttcagga aatagcagcg ggcttccggc aggtgaagag ccgtttatcg
    19381 tgccgttggc aattccgtta gtcgccgggc cgactattct cgccacgctg atgttgttgt
    19441 ctcatcagta cccgaatcag atgggacatc tggtaattgc tctgctgctg gcctggggtg
    19501 gcacctttgt catcctgcta cagtcttcgc tatttttacg tctgctgggc gagaaagggg
    19561 tgaatgcact tgaacgcctg atgggattga ttctggtgat gatggcaact cagatgttcc
    19621 tcgacggcat tcgaatgtgg atgaaggggt aaggttcaaa caggtgctgc acacaaccag
    19681 acgcggcacc tgttttatta cctgaactga taatgacctt cgcccaatcg gataaccctt
    19741 ccctcttctt ctgcgcggtt cagccacaca ctaatttgtt cgatggttaa accagtacaa
    19801 gacgccagcc tttcaatact tatcgattcc tttgcaagga tcgccagttc cgccacaaaa
    19861 agttgataaa aatcgacagg ggtatgcgag ggcatcactg cgtaatttgc gtcatcttcg
    19921 tgcagcgaaa acaattctgg ttgagcgtta cggagtgctt tgtcatacat cagcccagta
    19981 acgtctggca gtctgaacgt cacattctca gcctgttcag tactccatga cgccccattt
    20041 tctactaatc gtgaattggc tgattgcata tcctgatttg gtttaacctg cacaggcagc
    20101 cactgatgtt ttaatgcctc catcgccccg gttatcgttc caccggtcat tcccgcacgt
    20161 acaaccattg cgctttcagc aaggcaataa atatatttat ttcgcgccat cgcattgccg
    20221 acggtgaaac gtacctctgg gtaaaacggc gaaatcaaca ccaggttgcc tgctataagc
    20281 ccttcacgcc atttcactaa cgtactcgtt tttaacaagc tatcagctaa tacgccaacg
    20341 gcagttcccc cggcctccag tgccgacgcc attgcacatt catcaatacc tcgcgcacca
    20401 ccagagataa cgcaaatccc ctgttgggcc agtttcgctg ccagttgttg ggtatagcgc
    20461 aaatcggcag tcggagcatc tcgcgagcca acaatcgcca taccttccgc ttgcagtaat
    20521 gctttattcc cgcagccaaa taaaacggga ggtgcatccg ttcgcaatcg gtttttcaag
    20581 cgaacgggat aatcggcatc tccgcgggtt aagatccata aaccggcgcg ttgccactta
    20641 tctaccgcca acgccaaact atgaccacgc gccagcaagc caagaatacg ctcctgagaa
    20701 atacgcggat cgctccagcc tgtaagtttc tcttgcggtt gcggcaccag aagatcggcg
    20761 ggactgatac gttggtgctt cagccataat gcaaacttcc cccattcact attactaaga
    20821 ggtttatact cactcgccgc cgcgcgagaa aaatcgctgg ttagcaggag ggtcgcttgt
    20881 gcattggctg aaagattcat caatttttta ccgaggtaga cgcaagggca agaggataaa
    20941 ccgtcgggca acctgcctgg cgtaacagtg ctgtcagaac tgtcagcgtc catgcagaat
    21001 ccacgatatc gtcaaccagc aacgccggac ctggcatcaa aggagggata atcacaaacg
    21061 ccccgtcgag attttgacac tggtgaaaac ggttttgctg cattttctgc ggtggattgt
    21121 ccacgacttt ttcaatggca tcaataaaag gtaagccaag tttcgccgcc agtcgccggg
    21181 caaaatcagg aaccaggtcg aggtgcctta atgaaggaac gcagcatacc caggttggtt
    21241 caggatgcgg attccagcgt tggcaaacca tttccgcaca tgcttctacc agctcatcac
    21301 ttaagcgccc cgtttttttc tcctgtgcta cctgctttcc ccacccggaa tctccccaac
    21361 gagaaaggat tcgccccgta gatccttgta aacccgcagg caagttccct ttaaatccgt
    21421 attgagtaaa agccccagct gccatctgtt tattgagatt taacggcagg tcagcgtgtt
    21481 taataaataa atttgccgca tggagtaagc cgctatcaat gtcgggcgac aataacggat
    21541 gctgaagaca actgctacat ttaccacaac gttcggcact tggctcatcg agtgcacggc
    21601 gcagaaattg cattttgcac tccttggtcg tgatatagct ttcaacgtct gccagttcac
    21661 tttttctctg attcatcaaa tggttaattc gttcatgatc aggagaaaaa ctgacgatag
    21721 ttctgcgcca taatttctcg gtatacacca caggcgatgg attttccgct accaataatt
    21781 tcagtgcttt ttctatttgc ccataacgaa gattcgtccg ttgttcaatg cctcgtaggg
    21841 taagaccgtc attctcgcta agtacgttga gtatttcatg aatttgcgcc tccgcaggaa
    21901 aggcgctttc acggaagaat tgatgaatag cgcggtcttc accaccacaa agcaatatgc
    21961 caactgcgga atctatagca cgcccggcac gccccacctg ttggtagtaa ccgacaatag
    22021 accccggcat ctgataatga atgacgaaac ctaaatcagg tttatcaaac cccataccta
    22081 atgccgtcgt cgcgaccagc actttgattt tatttgccag cagtgcctgc tccagatatt
    22141 ccctggcagt attagaatct tccgcgcctt cacaagtcac gccgctgtag taagcaaatg
    22201 cactgatacc atttttcctt aaccactcgg cgacaagttc agcatcgcga gtcgtcaggg
    22261 tataaacaat tcccgatttg gaaaactgag ggataaccgt tgctaaccat gccagacggg
    22321 atgactgttc tcctaagact aaggcatcaa gcgccaaact ttcgcgagcc agcgttccac
    22381 gctgaatcac aatgtcaccc aattgctgac ggatatcctc aacaacacgg ttattcgctg
    22441 tcgcggttgt acccagaaca ggggtattcg caggcagttg gcgcaaaata tctaatatac
    22501 gtcggtaatc cgggcgaaaa tcgtggcccc aatcagagat gcagtgcgcc tcgtcgacca
    22561 caagcaagcc aatacgatcg gctataggat ataaaaccgt ttcgataaag tcctggtttg
    22621 ccaaacgttc cggggaaacc agcaagcaat cgacctcacc ttgcagcaac ttatcgctaa
    22681 tgcgctgcca ttcttccctg tttgtagaat tcaacgtttc agccgtaata cctaaccgtt
    22741 cagcggcggc aacctggtta cgcatcaggg caagcaaagg agagatgata attgtcgtgc
    22801 cagcgccgcg atcgcggaag attttactgg cgatgaagta cacggcactt ttcccccagc
    22861 cggtgcgctg tacaaccagc agttttcgcc gctgattaac cactgcatct atggcttccc
    22921 attgcccttc cctgaaagta gcggtggtat ttcctaacat ccgctgtaaa agcaattcag
    22981 ctccgtgctt ttccatcttt atctcctgcg atctttaggc agtgattaga acggattatt
    23041 agcaggtaaa tttgcgagag agatccaaaa gatgaagaga ggagataaga gaaatgaatt
    23101 actgcgagca agctcgcagt aatttgtcgc agtaattaac tcaacagctg gaacgcaatc
    23161 atcccaacga tggcaccaac agtgccgagt atggtttcca tcatggtcca ggttttcagc
    23221 gtttcggctt cggtcgcgcc ggtaaattta ccgaacaacc agaaacccgc gtcgttaacg
    23281 tggctgacaa caatcgaacc accggcgata caaatcgaca gcgccgccat ttgcgcaccg
    23341 gagtagttca gttgttcaat aaccggcatc accagtccca ccgccgttaa acaggcaacg
    23401 gtggcagaac cctgaatgat gcgcactgca gctgccagca cgaagcaggt gatggcaatc
    23461 ggcaggccca tgccggttaa cgcttcgccc agtgccggac ctacgccaga gtcaaccagc
    23521 acctgtttga atacgccgcc cgcaccaatc accagcagaa tgatccccgc cggttgcagc
    23581 gcgtgaccgc aaatttccat cactttatct ttcggcatgc cctgacgcat tgccaggcca
    23641 taaatcgcta ccagacaagc aaccagaatc gcggtaaacg gatgaccaat aaactcgaac
    23701 cattcgtaag cggtagagcc ttctggcaca aaacgcgcgg caatggtttt cagccctacc
    23761 agcaccagcg gcaacaggat cagcgacagg ctgaatccga aagatggcat tttgccttcg
    23821 ccgagatgcg gttcgctgat gtcgtcagga atatgcagct ccacgtagcg gctgatgaaa
    23881 ttaccccaca gcggcccggc aataatcatt cccggaattg ccgcacacag gccaatcagg
    23941 atcatccagc caaagtcggc gttcatctgc gatgccagca gcattggcgc tggtccaggc
    24001 accaggaacg cagcagcggc cgccacgcct gcaaataatg ggattaccag cttcaccagg
    24061 ttcgtaccgg tgtggcgcgc cattgagaaa gcaacgctaa tcagcagaac aatcgccact
    24121 tcaaagaaca acggcagcgc acagaccagc cccgcaaggc cgatggcata atgcgcgcgg
    24181 ctgtgaccga aggatttgag cattttgacg gcaatctgat cgactgcgcc ggtttcatgt
    24241 aagatcttgc caaacatggc tcccagggcg acaaccaccg ccaggaagcc gagggtgcct
    24301 cccatccctt tttccatcgt cgctgcgatt ttatcgagcg gcataccgga aaaaaggcca
    24361 gcccccatag acaccaccat taaagccagg aaagcgtgca tacgcgcctt catgactaaa
    24421 aacagcagca gtaaaacaga ccctactgct gttaaaacaa gcgttaatgt agtcactact
    24481 tatttgcctt ttttaataac ctcaatggtg cttgccacaa caccttccag cggttgatcg
    24541 atatctacca ccagtacatc ggtttcgtcc gcacccggct cctgcagcgt ttcaaactgc
    24601 gtcaccaaca tttgggtttt aaagaaatgg cctttgcgcg ctttcaggcg gctttcaatc
    24661 acatcaaaat cgcctttcag gtagatgaaa gagagattcg gattacccgc gcgtagcagg
    24721 tcacgatagt gttttttcag tgcagaacag acaatcagcg acactttatt agtgcgctgc
    24781 atagcaaacg cggcgtcgtt cagcgcctgc aaccacggtt tgcgatcgtc gtcattcagc
    24841 ggttcgccag acgccatttt ttcgatattg cagcgcggat ggaggaaatc gccatcaaga
    24901 aacgcggcat gaagttgatg cgccacttca ctggcgaccg cagatttgcc gctgcccgat
    24961 acgcccatca agacgtaaat gtggtgatca tggttagtcg tgctcaaagt ggtgccccca
    25021 caatacaaga attaaaaatg ttacgggtaa ctgttatcgg taacattgtc cagccggaca
    25081 atatcagaag caatatccat ccgtgcctta agtgtataag tgtgagctac ttcaaatttg
    25141 tgggcttaaa tagatccgcc cggtgacaag gtgaaaccta aatctaacat tttcggtgtc
    25201 acagattcgc cacgaatacg cgccagcagg cgttcagcgc caatactgcc catccgctca
    25261 cgcggcgtca gtacgctcgc caaccgaggt tccatcacct gaccaatgtc atgaccatgg
    25321 aaaccggcaa tcgccatatc gtcaggaact ttcaacccca gacgctggca ttcaaacgcc
    25381 gcgccgaccg ccaggtcatc attagtacag aacacgccat ccagctgcgg atattcccgc
    25441 ctcgcctggc gaatcaattc aataccggaa gagtaagaag aagattgctc aaccatcacg
    25501 ctatatggca ccaggcctgc atccagcatc gcctgttcgt atcccttctg tttgatgata
    25561 gtacgttcgt cgagacgtgc gccgagatag gcaatgtggc gatgcccgcg agcaataatg
    25621 gcggtggtca tctggcgtgc cgcttcaaag ttatcaaaac cgacggcaat atcgaggcac
    25681 ggcgacttgc tgtccatcag ttccaccact ggaatccccg ccacttcaat catctttaag
    25741 gtgcgcggcg tgtgcgtacg ttcagtgagg atcaggccgt cgatattcca ggagagcatg
    25801 gattcgaggc gttcttgctc catttccggt ttataaccgt agtgggccag catggtctga
    25861 taaccgtgcg cgtcggtgac gctttcgatt ccgcgtaata cttccgcaaa aacctggttg
    25921 gtgagagaag gtaacaggac gccaatcgcc cggctggtgg cgttagagag gatatcgggt
    25981 gcgcgattgg gaatataacc cagttcatca agagcggcgg caatcttgcc gcgtagagcg
    26041 acggaaacct gctccgggtt gcgtaaaaaa cggctgaccg tcattttggt cacgcctaca
    26101 cggtcagcca catcctgaag tacgggtctt ttctttttca tcgtcctgaa ggtacaaaag
    26161 agatagattc ttttagttta acacggacgc ctcagaacct tcccggggaa aacagtgctt
    26221 gtttacaatt attagatata cactaaatca attgcatcgc attgtgctaa cgacgcggca
    26281 aaaccccggt cgcgtacatc agcacgcgac cggagcgagc gaggaagcct gcaaaaaggc
    26341 aggcttcaaa gatgccgttg agttttaaac cggcggcaga tcgaacagta acacttcgct
    26401 gtcgctatcc gcatgaatgg agattgcctg ctcatcccag attgccagac catcgctggt
    26461 cgaggctttc acgccgttaa tggtgacatt gcctttcacc acctggatcc agacgcggcg
    26521 ttcagcggca atctgatgca ccgactgctc atctttcagc aatgcccaac gatacagttc
    26581 catatcctga tgcactttca gcgaaccatc tcgcgcatcc ggcgagagca ccagctgttt
    26641 gccctgtacg gcatcgaagc gacgctgttc ataacgcggc gtaatgccgt tttcttcagg
    26701 catgatccag atctgataca gatgcagacg ctcggtgctg cttgggttgt actcagagtg
    26761 acggataccc gtacccgcac tcataatctg gaactcaccc gccggaacct gctctttatt
    26821 gcccatgctg tcctggtgct caacagtacc ttccagcacg taggtcaaaa tttccatatc
    26881 tttatgcggg tgagtgccaa agccctgccc tgcttcaatc acgtcgtcgt taatcacgcg
    26941 cagcgcggag aagcccataa agttcggatc gtaatagttg gcaaaagaga aagtatgcca
    27001 ggaatccagc cagccatgat ttgcatgacc acgttcattt gctttgcgta agtagatcat
    27061 ttgtattcgc cccctgaatg ttttcgatgg gcttagtgtg gacccgatct gcctgggatg
    27121 atagagggtg aaaattgacc cctctgttca aaaaaattga actattcgag gggccatcaa
    27181 acttacttcg cgagagtcac tgtggaggga gaggcttgct caaaaccgcg ttcaaggatt
    27241 tccaaattgg taagaacttc agattccttg acgtaatttg gcgcaccgtt ggtgatggtt
    27301 tgatacaacg catcataaac gcgcccgtaa tcgcccacct ccggcttcat ctcttctctg
    27361 accgtcacac cctcgtcatt gacatactcc agcacaccga ccgaatcatc cgctgcgaat
    27421 cccggttcgc ccggcataat attagccttc aggctggttt cctgctggtc gataccgtat
    27481 ttaataaacg aacctttctt accgtgaacg ataaatttcg gataatcgat tttcaccaga
    27541 tggctggttt tgacgatggc ttttagatcg ccataaaaca gctgcgcttc aaaggtgtcg
    27601 tccggattgg ctttattacg caggctgcgg atgtcataag cgacgtgatc cgggcgaccg
    27661 aacagagaaa taatctggtc catcgtatgc acaccaaggc catagaacgc gccatcctgc
    27721 ggcagcccag gtttggtttc tgccaccggg cggtaatagt caaaatggct ttccacttcg
    27781 acaatctctc ccagcttgcc gctttcaatc gcttttttcg ctgtcaggaa acaggaatca
    27841 aagcgacgat tctgatacgg cgtaacgatc agccctttgc ttttcgccag tgcaaacagc
    27901 tctttcgcct gcgcaattgt cggagtgaac ggtttttcga ccagcacatt tttcccggct
    27961 tccagcgcgc gcttcgcgta ctcgaagtgg ctgtccgcgt gggtgcagac gacaaccagc
    28021 ttaacatcgg gatcgtttag cacttcgtcg agatcgctgg tgaaatggat atgggaataa
    28081 atgggggcct gttcttccgg cttcgcatgg cggcgaaaaa tatgcgcgac atgccagcta
    28141 tccttgcggt taagtacata cggcagatgg taacgggtgg tgcttttgcc gaagccaata
    28201 aaggcgcagt taatgaccat gatttcgtcc tttttaaggt ggttatttac accttagcgc
    28261 aaagcggacg tggttcctac tggagtgcgc ataacgaaca caagcacttc ccgaggataa
    28321 attgagaacg aaaggtcaaa aaaaaagcca gcacccggct ggctaagtaa tactggaagc
    28381 aatgtgagca atgtcgtgct ttcaggttct ccgcgagggt cttcctgatc gcgagacaat
    28441 aataatcatt ctcattcgca cttgtccaac actttttgca aaaaaatgca tttgactcgc
    28501 atttgaaagt caatgatgtt gaaagggaca tttaccccaa agaggacaaa ggaatgagtg
    28561 agatagtaat acgccacgca gaaacacgcg attacgaggc catcaggcag attcacgccc
    28621 agccggaggt gtattacaac acgctacagg tgcctcatcc ttcagaacaa atgtggctgg
    28681 aacgactcac cgctcgcccc ggcatcaagc agttagtcgc ctgtattgat ggaatcgtcg
    28741 taggccatct caccattgac gtgcaacaac gcccacaccg cagtcatgtt gccgactttg
    28801 gtatctgtgt cgactcccgt tggaagaacc gcggcgtcgc cagcgccctg atgcgagaga
    28861 tgattgaaat gtgcgacaac tggttgcggg tagatcgtat tgagctaacg gtgttcgtcg
    28921 ataacgcgcc agcaattaag gtctataaaa aatttggctt tgagattgaa gggactggca
    28981 agaagtacgc gctgcgtaat ggtgaatatg tcgatgcgta ttatatggcg cgggtgaaat
    29041 agagaatttg caatctacgt ttgtcattta agccctcacc taccgggaga gggcttaaga
    29101 tattaatacc ccgcagttaa atcatccacc gagcgtgggt cggatgcacc gtacagctca
    29161 ccgtccggcc caaccataat gctttgtgta ctgcccatcg cctctttcag cgccactttc
    29221 tgaccttttg cttccagcag cttgatcgta tccgggctaa accctttttc gacacgcagc
    29281 tcgtccggca accactgatg gtggaaacgc ggcgcattgg tcgcttcggc gacgttcatt
    29341 ccataatcga tgctattcac caccatttgc agcacagtgg tgataatccg gctaccgcct
    29401 gggcttccgg tgaccagcca ggttttacca tccttgacca caatggttgg tgacatcgac
    29461 gacagcgggc gtttattcgg cccgacggcg ttggcatcgc cgcccaccag cccgtaaacg
    29521 ttcggtacgc ccggtttggc ggagaaatca tccatctggt tattaagcag aataccgctc
    29581 tcgcccgcga caatgcccgt accgaaggtg gtgttcagcg tataggtcac cgccaccgcg
    29641 ttaccgtctt tatccaccac tgagtaatgg gtagtttgat tactctcata aggcgcaagc
    29701 ttgccggggc gaatttcgct ggatggcttc gctttattga tatcgatttg atcggcaata
    29761 gatttggcat aggctttatt ggtcagcgcc tgccacggta ctttgacaaa atccggatcg
    29821 ccaagatatt ccgagcggtc ggcgtaggcg tatttctcag cttctgccat gatttgcatc
    29881 gcgtcggcac tgccaaagcc atatttcttc atatcgaagt tttccaggat attgaggatc
    29941 tgcacgatat gaatcccacc ggaagatggc ggtggcatcg agtaaacctg atacccgcga
    30001 tattcgccgc taatcggtgt acgttcgacc gctttatacg ccgctaaatc ttctttagtg
    30061 atcaagccac cgtttttctg catctcctgg gcaatctgtt ccgctatcgt gcctttgtag
    30121 aattcgtccg ggccgttttc ggcaatcatc tccaggctct ttgccaggtt cgcctgcacc
    30181 agcttgtcac cctttttcag cggcgcgccc tctttccaga agatagcctt gctgttttcg
    30241 tgattcggca atacttcgct accgtaggtt ttgagatcgt cagccaacgc gtcgttaacg
    30301 ataaaaccat cgcgtgccag tttaaacgcc ggttgcacca ctttgttcaa cggcatggtg
    30361 ccgtacttct ccaacgccag cgagaaacct gctaccgtac cgggcgtacc agaagccaga
    30421 tgcgaagtga gtgatttttt gctgtccggg ttgccctgat catcaaggaa catatcgcgg
    30481 gtcgctttgg cgggtgccat ttcgcggaaa tcgatagcgg tggtattgcc atttttcgag
    30541 cggatcagca taaaaccacc accgcccaaa tttcctgcct gcggatgcgt taccgccagc
    30601 gcgtagccca ctgccacggc agcatcaacg gcattcccgc cctcctttag aatatctacc
    30661 cccacttggg tggcagtggc atcgacagac gccaccatcc cctgtttcgc gcgtaccgga
    30721 tgaaagacat cttcctccac gccgtaagaa acaggagacg gcgcaggtgg cgcggctacc
    30781 gtactaaaac aacttcctgt gaacagagca gcaatggcca cccggcgtaa aaacgtcggt
    30841 tttatcatcg ttattctcca gagattaagg ggcaacccca gctaagcctg gtatataact
    30901 ctgaattaat cctgatgatt gcaggaaagg agtaaactta aagaatatct tcagaggagg
    30961 atacgcgatg aaacgacttc tgattctaac ggcactcctg ccgtttgtcg gctttgcaca
    31021 gcccattaat actctgaaca accctaacca gccggggtat cagatcccca gtcagcagcg
    31081 gatgcaaacc caaatgcaaa cgcagcaaat ccagcaaaaa gggatgctga atcagcaact
    31141 gaaaacgcaa actcagttgc aacaacagca tttagaaaac cagataaaca ataattctca
    31201 gcgggtgttg cagtcacagc cgggggagcg aaatcccgcc cggcagcaaa tgctgcccaa
    31261 caccaatggc gggatgttaa acagcaaccg taatccggat agttcgttga atcagcagca
    31321 tatgttgccg gagaggagaa acggcgacat gctgaatcaa cccggcacgc cgcagcctga
    31381 tataccgttg aaaactattg ggccgtaaag ttcggaccaa tcacatcaat cgcatcggta
    31441 cagatgcaat ccacgcccca gcgcagcaac tctgccgcgc gctggggttt attgacggta
    31501 taaaccagaa tccgtaatcc ggcgtctttc aactgcatca ctcgcgcttt atcgagcaac
    31561 ttatgattga gatgaataga gacgcagccc agtcgcgcgg tcaattctcg ccagtcgtcg
    31621 cgccactcat caagcaataa accgcgcggc agttctggta ctgcttgttg cgcggcgtct
    31681 aaagcatcaa tctcaaacga tgacagcagc ggcggcgtca ttccggccca cagttcgcgt
    31741 gccgccagcg ccaccatttt gcccgttaat ggcccggtgc cagtggtggg tttgatttcg
    31801 atattcgcca tcatcccgtg tttgcgacaa cgttccgcca cctgcgaaag caacggcagt
    31861 ggctcacctt taaacgcttt gctgtaccaa ctgcctgcat ccacgcgcag taaatcctgc
    31921 cagttcagat cgcccgcgac gccccagccg ttgctggtgc gttcaagatt gtcgtcatgg
    31981 agcaggaaga tctcgccatc tttcgataac ttcgcgtcaa attcgatcat cttatgaccg
    32041 tattttgccc cgacgtcgat tgctgccagg gtgttctccg gggccagttt accgccgcca
    32101 cgatgggcga cgatgcgggg ataaggccag ttactcatac tcgttgtcct gtttcaccat
    32161 caaaaagatg tagctgattt tccggcagat gcagccacag cgtgctgcct gccgtcgggc
    32221 gctcctgatg tgccagtcgc accaccagct tctgttcgcc ccagcgtccg tgcgccaggt
    32281 tatctgcgcc gaggatctcc agcgtgtcca tcaccagcgg cacaccacct tctgcctgcg
    32341 agcttagcgc tatatgttcc gggcgaatgc cgagggtcat tttacgcccg gcatactgac
    32401 ggtagccacc gtttagcggc agcgtaatac caccatccag ttcgaaatgc gtaccttcgt
    32461 tattcacgcg gccagccagt aggttcatcg ccgggctgcc gataaaactc gctacaaaca
    32521 ggctggcggg cttttcgtag acttccactg gcgtgccaat ctgttcggca acgccgccgt
    32581 tcatcaccat tactcgctgg gcgagcgtca tcgcctcaac ctgatcgtgg gtaacgtaga
    32641 gtgaagtcgt tttcaggcga cggtgcagct gttgcagttc aagacgcatc tgcacacgca
    32701 gcttggcatc gaggttagag agcggctcat caaacaggaa cactgccggc tcacgcacaa
    32761 tcgcacgccc catcgccaca cgctgacgct gaccgccaga tagctcacgc gggcggcgct
    32821 taagcaggcc gtccagctcc agaatgcgcg ccgcttcttt aacacgttcg gcaatttgtt
    32881 gcttgcccat gccgcgaatt ttcagccccc acgccatgtt ctcctctacg ctcatatgcg
    32941 gatagagcgc gtagttctgg aacaccatcg caattccgcg atctttcggc tccatttcgg
    33001 tgacacgctt gcggtcgatc cagatatcgc ccgtcgttac ccgctccagt ccggccacca
    33061 ttcgcaataa cgtcgatttc ccgcagccag acggcccgac catcacgata aattcgccat
    33121 ccgccacatc aagggtcagc ggtttaatga cctgggtttt gccatcccag cttttggtta
    33181 ctgcctgtaa tttcagtcct gccatcttat ttctcactat cgaccaggcc gcgcacgaag
    33241 gcacgctgca tcactaaaac aatcactact ggtgggatca gggttaacaa catcgccgcc
    33301 atcaccgagt tccattccgt ggtgccttcg cctgtggcga tcatcccttt aatccctgct
    33361 acggtggtgc cgagatccac atcggtaata atcaacaacg gccacaaata ctggttccag
    33421 ccgtaaataa aggtgatcac aaacagcgcc gccagattag ttttggacag cggaaaaacg
    33481 atgtcgcaaa agaaacgcat tggtgacgca ccgtcgatcc gcgccgcttc caccagctca
    33541 tccggcagcg tcataaagaa ctggcggaac aggaaggtcg cggtcgccga ggccatcagc
    33601 ggcagcgtta aacctgcgta gctgtcgagc attttcaggt tagcgatgac ttccaccgtc
    33661 gggaagatac gcacttcaac cggcagcatc agggtgataa aaatcatcca gaagaagagg
    33721 ttacgtagcg gaaaacgaaa ccagacaatg gcaaatgccg agagcatcga gacggtaatt
    33781 ttgccgagcg taatgctgaa cgccatcacg aagctgttaa gcaacatccg ccagaacggc
    33841 gcactattcg tgcctacccc gtttacccag atgttgtgga tgttttccag cagatgtgtg
    33901 ccggggatta gcgtcatcgg cgcggcatag acttcttgtt tatccagtgt cgccgcgaca
    33961 aacgccacgt acagcgggaa gaggatcacc gcgatcccga ggatcagcat ggtatggctg
    34021 aatattgtca gccacggacg gttctcaatc attggtaacg taccttgctt tcaacatagc
    34081 ggaactgcac caccgtcagc acgatgacga ggaacatcaa caccaccgac tgcgcggcag
    34141 acgaagccaa atccagtccg gtaaagcctt cacggtagat cttataaatc agcgtcgtgg
    34201 tggcctgtac tggcccgccg gacgtggcgg cgtcgatcac cgggaaggtg tcgaagaagg
    34261 catacaccag gttcaccacc agcaggaaga aactcaccgg agcgataagc ggcagcgcaa
    34321 tcttaaagaa gcggcgaatc gggccagccc catcaatggc tgcggcttcg atcaacgaac
    34381 gggggatgga ttgcagcgcg gcatagaaga acagaaagtt gtagctgatt tgcttccaca
    34441 ctgaggcaaa caccaccagg aacatcgcct gaccgctgtt ttgcgcgtgg ttccagtcgt
    34501 agccgaactc agcgagaaaa tgggtgatca gcccgcgacc ggggttaaac aggaagatcc
    34561 ataatacggc ggcaacggcg ggagccacgg catacggcag cagcattaag gtttgataga
    34621 aacggctgcc gcgcacgatg tactccacca acgccgcgaa gaacagtgac accagcaaac
    34681 cgctgacggt gacaaaggtg ctgaatttta tcgtcgtcca gaaggcgtcg agatagtagc
    34741 tgtcgtgaaa cagcgtgacg aagttatcca ggccgacaaa ctggctggag aaaccaaatg
    34801 gatcgacact ttgcagcgag taccacaacg cttcgcccgc aggccagata aaaaagataa
    34861 cggtgatgat gagctgcggc gcgaccagca gataaggcag ccagcgcgag cggaacaccg
    34921 gacgggatga tgacattaca gttaattcct gaactgtgcc ggatgcgctt cgcttatccg
    34981 gcctacacgc gatgccaggt cagataagcg aaacgcatcc gacattacac tgattaagac
    35041 ttcgtcgatt tctcaaagcg gcgcagtaac tggttcccac gctcaacggc agtatccagc
    35101 gcctgctgtg gtgtcttctt accggtccac acgctctcca gctcttcatc cacaatcacg
    35161 cggatctgcg gcatgttgcc cagacgcaaa cctttggtga acggcaacgg tggcttgttc
    35221 agcatctgac gcgtggcaat atccgctcct gggtttttct cgtaaaagcc ctgctcacgg
    35281 gtcaggtcat acgccgcttt agtgattggc agatagccgg ttttctgatg ccactcggca
    35341 gcgttttctg gctttgcgag gaagtcgagg aacttcgcca cgccggtgta ggtttcttta
    35401 tctttaccct gcattaccca tagactggct ccgccgataa tggcgttttg cggcgcgtct
    35461 ttcgcatcgg catcgtaagg catcatgcct acgccatagt taaatttggc gtactcgcga
    35521 atgttggcaa gagaaccgga agaggcggtc gtcatcgcgc aatcaccgtt atagaacttc
    35581 tcggtggatt catccttacg cccgacgtag ctgaaatcgc ccttcttgtt catctcttcg
    35641 agcatagcga tgtgtttcac ctgctccggc ttgttgaact ccagcaccgc gtctgtgccg
    35701 tcaaagccgt tgtttttgct ggcaaacggc agaccgttcc aggcgctaaa gttttccagt
    35761 tggatccagc cctgccagcc gctggcgtag ccgcacttca taccggaggc tttcagtttc
    35821 gcggcatagt ccgccagatc ctgccaggtt ttcggcggct gttccgggtc taaaccggct
    35881 ttcttgaagg cgtctttgtt gtaatagaga acgggagtcg agctgttgaa cggctgggag
    35941 agtaagtgcc cagttttgct gtcggagtag tagcctgaaa ccgtcggcac aaactgcgac
    36001 tcatcgaact gaatccccgc ctctttaaac acgtcatata ccggtttaat ggctttcgac
    36061 gccatcatgg tggcggtgcc aacttcataa acctgcaaaa tagccggagc gttgccggta
    36121 cgaaatgcgg caatccccgc gcttaaattc tgttcgtagt tgcctttata ggtcggtaca
    36181 attttgtaat ccgggttttc ggcgttaaaa cgttgggcca gagaatccac ctctttaccc
    36241 agttcccctt ccatagaatg ccagaacgga atggtcgtcg ctgcctgcgc atttgccatt
    36301 aaagccagtc cgagcgccag tgctgaagct gtataacgta acggtttcat tgattatctc
    36361 tcttgttgta ccgaatgcgc gaattcacgc gttttatgct cgcggggtaa catgacatgc
    36421 tcgaattaca gaaaaataac tattttgtta catttttaag atagtaaggt gtcagaaaga
    36481 tgacaaggcg gtgacggtac gggtgaggga aaatgggaga tggggcacgg atatcccgag
    36541 aatgcactga aaagaggcga ttaacggtca gctaatcgcc agaactcctt accatgaaga
    36601 gcaaaaatgg cagcaaattc tgcgtccagt tttgctgccc gatgagagct acatttttca
    36661 attttaacaa aagattgccg cgacactggt ctaacggcaa agaagatttt cattgcacca
    36721 cactctgctt cagccgttca ttggcggtct taatccattc ccggtggtgt tttctataat
    36781 gtggttttga acgtttagcc gtatcaacca cagcaatata ctgctcgttg gcttcacgta
    36841 accgactcat tttcgccaac atttccgcat agtaaatacg cgcctgcggg ccggagtaat
    36901 aagaaatcaa cacctcaaac tcgctttccg catcagcgta tttttcttgt gcggctaacg
    36961 ctctggcaaa taacaaatgc ccgtctgccg actgaaaatc ggggttataa cgcatcacat
    37021 cttccagcgt ctgctgacag gcggcgaact cctgaatggc aaactgcgcc tgcgccaggc
    37081 ttagcatcat tgccgcttca tggacgaata tcccgcttaa cgcctgctga taatggggaa
    37141 cagcttcgtg ataacgcccc agacacacca gttcattagc caacgcataa tggttttgat
    37201 tcgtctcggc aatcgccagt tcttcggtta atttgcgcag gtggcgctca gggttgagtt
    37261 tgtcctgcag gcgcatcagc aaagtatgcc cgtgacggtc agcgcccgtt tcaggcaaca
    37321 tcactatgac gaaatacgcc acgcagccaa tcaccggaaa acagaaaatg ataaaaatcc
    37381 agtaccgctc ttgtccggta cggtaagcgt gaatacagca aagcagttca agcatgacaa
    37441 caacggcata atgcatggga gcaaaccttt ccgtggcaaa taagtggtct ggttttaatc
    37501 ctcccggtct gttgaaacaa gtctgtatac ggacgaataa cccggcagaa cttagctgcc
    37561 gggttattga taatttaccc gcctaaatac gcacttctca ccgcttcatt cgccagtagt
    37621 gcatcaccag tatcggagag caccacatga ccgttttcca gcacgtagcc gcgatcagcg
    37681 agcttcagcg cctggttggc gttctgctcg acgaggaaga tggtcatccc ctgctcgcgc
    37741 agttgttcga tggtgtcgaa aatttgctgg atgataatcg gtgccagccc gagcgatggc
    37801 tcatcaagca acaacaaacg cgggttgctc atcagcgcgc gcccaatcgc cagcatctgc
    37861 tgctcaccgc cggacatggt gcccgcccgc tgaacacggc gttcatggag acgcggaaac
    37921 agttcataca cccactttat gcgttcctgg aactggtcgc gttcggcaaa aaagccgccc
    37981 attgccaggt tctcttccac cgtcatccgc gagaaaacac gacgcccttc cggaacaatc
    38041 gccaccgctt cgcgcatgat tttcgctgtc tgccagtcgg taatgtcttt atcatcaaac
    38101 acaattcgcc cactggtggc gcgcggatcg ccgcataacg tgccgagcaa ggtggttttc
    38161 cccgccccgt tcgcgccaat gagggtgaca atctcgccct gattgatatg caggctcacc
    38221 tcatgcagcg cctggatttt gccgtagtgg gcgctgactt tgtcaaagga caacatgact
    38281 ttttccatct tatgcctcac ctaaataggc acggatcacg tccgggttat tacggatctg
    38341 ctccggtgta ccgtttgcca gcggcgtccc ctgattgacc acgtaaattc ggtctgaaat
    38401 tcccatcacc agcttcatat cgtgttcaat caacaagata gtggtgttgt gatgattacg
    38461 cagttcggca atcagctcat ccagctcttt cgtctctttc gggttaagtc ctgctgccgg
    38521 ttcgtcgagc attaaaatct ccggctgcgt caccatgcag cgggcgatct ccagacgacg
    38581 ctggtcgcca taggccaggt tactcgcctg acggttggcg tgttccaaca aaccaatgcg
    38641 ctcaagccag gtagcggcgc ggtcgagcgc ttcgctctgg gcgcgacgga aagaaggcgt
    38701 tttcaacagg ccagagaaca gcccggtttt cagttgctga tgctgcgcca ccagcaggtt
    38761 ttcaattacc gtcatttcac ggaacagacg cacatgctgg aaggtgcgca ccacgcccat
    38821 tcgggcgatt tgctgccccg gtaaaccttc cagatgctga tcgcgcagta aaatggtgcc
    38881 gccggtgggt ttatagaatc cggtcaggca gttgaagacc gtggtttttc cggcaccgtt
    38941 agggccgatt aacgagacga tctcctgcgg gtacagttca agattgacgt tgttcaccgc
    39001 cagcaggccg ccgaagcgca tcatcaagcc gttaacagat aataatggct gactcatgcc
    39061 tgctctcctt tcgctgcgcc gttcttcagc ttgagttgcg ggcgcgtcat gggcagcaag
    39121 ccctgcggac gccagatcat catcagcacc atcaaaccac caagcattaa catgctgtat
    39181 tcgttgaaat cacgcatcaa ttcgcgcgac accaccagca aaattgccgc cagaattacc
    39241 gcaaattgcg agcccatacc gccgagcacc actatcgcca gcacaaacgc cgattcggca
    39301 aaggtgaagg attccgggct gacaaagccc tgacgcgcag caaacaacgt tccggcaaaa
    39361 ccggcaaacg cagcgctgat ggtaaaggca gtcagcttga tacgacgcgg gcttaagccc
    39421 agcgaacgac aagcgatttc atcttcacgc aacgcttccc acgcacgccc cagcggcatc
    39481 cgcagcaggc ggttaatgac aaacaggctt aacaccacca gcagcaacgc caccaggtag
    39541 aggaagatga cgcgatcgga gggatcgtat ttcaggccaa agaaattgct gaacgtgtcc
    39601 cagccgcctt cacgggcggt acggctgaac tcaaggccga agaaagtcgg tttcgggatc
    39661 tgactgattc cgttcgggcc gccggtaatt tcggtgttat tgagcagcaa tatgcgcacg
    39721 atttcgccga acccgagggt aacgatcgcc agatagtcac cgcgcagacg cagcaccgga
    39781 aaaccgagca ggaagcccgc cgccgccgcc atcaacccgg caatcggcag acaggtccag
    39841 aagcccaagc cgtaatagtg attgagcagc gcaaaagtgt aagcgccgat agcgtaaaaa
    39901 ccgccgtatc ccagcaccag cagaccagaa agacctacca ccacgttcag cccgagaccg
    39961 agaataatgt agatcatggt cagggtggcg atatccaccg tcccacgtga aaccataaac
    40021 ggccacgcca ccgcaagcac cagcagcgcc acgagaaaca gcttttgctt caccgtggag
    40081 ccatcaatcg cgggcagaat aaacttcggc ccggaaacgc tttttagccc tttctggaaa
    40141 gccggtcgca aaagctggaa gaaaaagacc accgccgtgc cgataaacac ccactgccaa
    40201 cggatctctg aagccgtgtc gaccaccagt ttggtgccat ccagctccag ttgcacgccc
    40261 ataaagacgc ccgccagcac aaagaacatg gcggcagaga gcagcgccat tgcaatatgc
    40321 atcggtttca tactttctct acctccgggc gacccagaat accggtcggc atcaccagca
    40381 acaccagaat cagcagggcg aatgagacca catctttata ttccgtactc agataggcag
    40441 aagagagcgc ctccgcaatc cccagaatca ggccgccaat catcgctccc ggaatgctgc
    40501 caatcccacc gagcaccgca gcggtaaagg ctttcatccc ggccataaag ccgatgtagg
    40561 ggttaattac gccgtagaac tgaccgagca gcacacccgc caccgccgcc atcgccgcgc
    40621 caatcacaaa ggtcagcgca atcacccggt cggtgttgat gccaagcagg ctcgccattt
    40681 tcagatcttc cgcgcaggca cggcaggctc gccccatacg ggaatagcga atgaaaatcg
    40741 tcagcgccag catggcgagg aaagtaacaa tccagataac cgcctgcatg gtagtaatag
    40801 aggcagagaa gttttcgcta tggcctacca cccactgacc gttaaacagg ctcggcagcg
    40861 ccacgtcgcg cgaaccttcg gtcaggctga cgtagttttg caggaagata gacatgccga
    40921 ttgcggaaat aagcgcaatc aggcgcttag agttacgcac cgggcgatag gccacccgtt
    40981 cgatactcca gccgtaggcg ctggcaatga cgattgcgcc aacgaatccc gccgccacca
    41041 gcagccagcc ggtatcaatt cccagcatca tcagtgcagc gatgatcata aaggagacgt
    41101 agctgccaat catgtaaacc tcgccgtggg cgaagttgat catgccgata atgccgtaaa
    41161 ccatggtgta gccgatggct atcagcgcgt aggtactgcc cagcgtgacg ccgttaaaca
    41221 tctgctgcaa gaaatacaaa aactgctcag acataaggta acctttctaa acccgcccgt
    41281 ttttcacggg cagtgggatg atcacttggc tgccgtggat gaaccgtcgg cgtgccactg
    41341 gaagacacca aaatcaaatc ccttaagatc gcctttttca tcccagttca gcggcccaat
    41401 tacggtgttt gcaccgttag cttttaaatc tttcaccagc gccagcggct catcgctgcc
    41461 ggtacgctca agtgcagtcg ccagagattg caccgccgcg taagtgatcc agacatatgg
    41521 cccggacgga tctttcttgt ctgctttcag cgcatcaacg atgccctgat ttgccggatc
    41581 ctggtcatag cgttttggca tagtgaccaa catgccttcg gcagcatcgc cagcaatatt
    41641 cgacaacgac gcattaccca caccttccgg ccccataaac tgggttttca ggccaacgga
    41701 acgggcctgg cgcagcatct gccccatttc cgggtagtaa ccgccgtagt aaacgaagtc
    41761 gatgttttct tttttcaggc gggcgatcag cgcggagaaa tctttctcac ccgcggtaat
    41821 accgtcgaag aagacaacgt tggcgttagc cgctttcagc ccgtcctgca ccgaacgtgc
    41881 cagcccttcg ccatactgtt gtttgtcgtg aatgatggcg atgcgctggg gcttcaccgt
    41941 ctcaacaatg tattttgccg ccgttggccc ctgggaagaa tccagcccgg cagtacgcat
    42001 gatgtattga taaccgcgtt gggtcagctc cgggttggtc gcccccggcg agatcatcaa
    42061 aataccttcg tcttcgtaga tatctgatgc aggttgggta gaagaagaac acagatgacc
    42121 aataacgtat ttaatgccgt cgttaacgat tttgttggcg accgcaacgg cttgtttcgg
    42181 gtcgcaggcg tcgtcatatt ccacgccaac cagtttatcg cccttaattc cccctttggc
    42241 attaatgtct ttaattgcct gacgcgcgcc gttaaattcc atatcgcccc actgggcaat
    42301 cgggccggac atcgcgccga caacggcgac tttaatatcg tcagccatag cggtgtgtga
    42361 aattgtcagt gcaatcatcc ctgcgatgat agttttcgca ttccgtttca tagtcaaaaa
    42421 tccccattcg tgatgttgtg ttgctttgtt tttatgtgtt aacaaatcag actgttcttt
    42481 ttttatactg cactgttttt acctgtctga ttaagggttt agcgcagtat tttgtgatga
    42541 tagcgattaa aatccctatt tttcagtcga ttaagaacag ataatattct gaatttattg
    42601 atagataaac agaaaagagc gccgttgtca gcataaaata acggtacaaa gggcagaata
    42661 attcactacc atttagggga ttatgctgga tatttttcat tctctaatgt tttaactttg
    42721 taattattgc tgaaaaaaaa ttaatcacct gccaaaagaa ataaaaaaga gaaagcctcc
    42781 gattaaatta tttcgctaca ctggttccac ttttgtgatt tacacgggtt ccccatgaag
    42841 ctgaccatca ttcgattaga aaactttagc gaccaggacc ggattgacct gcaaaagatc
    42901 tggccggaat attccccttc ctctttacag gttgacgata accaccgcat ctacgccgcg
    42961 cgttttaacg agcgcctgct cgcggccgtg cgggtaacct taagcggcac tgagggggca
    43021 ctggattccc tgcgcgtgcg ggaagtcacc cgccgtcgcg gtgtggggca atatctgctg
    43081 gaagaggttt tgcgtaccaa tcctggcgtt tcatgttggt ggatggcgga tgctggcgtg
    43141 gaagatcgcg gcgtgatgac ggcgtttatg cgagcactgg gatttacggc acaacagggt
    43201 ggctgggaga agcgttaatc gtcaagtttg atttcacaag tgatattgcc tgatgcgcta
    43261 cgcttatcag gcctacaatg tgtgctgcaa tttactgatt tctttgaatc ttgtaggccg
    43321 gataaggcgt ttacgccgta tccggcatga agcaacgtac tcgatattag caatttggcg
    43381 gcaacccaaa gttgccgatt aatgattact tcgcatcggt cgccgtgccg ttggcgtgcc
    43441 agtcaaatac gccgaactca aagcctttca gatcgccttt ctcatcccag ctaagcggcc
    43501 ccatcaccgt atcaaccgag ttcgctttca ggtatttagc gatctccgcc ggatcgtcag
    43561 actgattcag ccccgcttgc aaagattgca gcgcggcgta ggtggtccag acgaacgcgc
    43621 cgcttgggtc ctgttttttc gctttgatcg catcaacaat gggtttgttc gccggaacct
    43681 gatcgtagtt cttcggcttg gtcaccagca gcccttccgc tgattcgccc gcaatgttag
    43741 acagcgaaac gttagccaca ccttccggcc ccataaactg agttttcaac cctgccgcgc
    43801 gtgcctgacg caggatttgc cccatttccg ggtgataacc accgtagtaa acgaagtcga
    43861 tattctcttt cttcagacgc gccaccaggg ttgagaaatc tttttcccca gcggtaatac
    43921 catcaaagaa caccacgtta gcattgcctt tcttcaggcc gtcctgcacc gcacgcgcca
    43981 gaccttcgcc gtactgctgt ttgtcgtgaa cgatagcaat gcgttgcggt ttcactttct
    44041 caagaatata tttcgccgcc gtcggcccct ggtcggagtc caggccggta gtgcgcagga
    44101 tcagctgata gccacgggcg gtcagctctg gcgcggttgc cgctggggtg atcattaaaa
    44161 tgccttcatc ttcatagatg tctgacgctg gctgcgttga tgaagaacag aggtgaccaa
    44221 tcacatattt aatgccgtcg ttaacgactt tgttcgccac cgcaaccgcc tgtttcgggt
    44281 cacaggcatc gtcatatttc acgatttgca gtttgttgcc tttgatacca cctttagcat
    44341 tgatatccgc aaccgcctgc tcagcaccag taaactcctg gtcaccgtac tgcgccaccg
    44401 gaccggacat tgcgcctacg acggcgactt taatatcttc tgccagagcc atattgctga
    44461 atgccagcgc gatacatcct gccagtaacg ctttaccctt tatgttcatc ctgagaaccc
    44521 ccactcttct ggttattacg tgtgttgtga tgttgttgtg cagcacttta tttcgtttta
    44581 tacgtagcca cccgtgcttt agcagcatac tctgctaaaa cataccccat ttttatgata
    44641 ttggaatagc tattttgaca gtttattaac aatctgcgtg gggattggcg ttttgccgga
    44701 gggggaaact gattattgta gaggcccaaa aaacaaaaac cccggactct catccagggt
    44761 tctctgctta atagcggaaa ttacgcttca atggcagcgc gcaatttttt catcgcgttc
    44821 ttttccagct gacgcacacg ctcagcggaa acaccgtaac ggtcagccag ttcctgcaac
    44881 gtggacttgt tgtcttcgtc cagccagcgc gcgcggatga tatcctggct acgctcgtcc
    44941 agaccttgca tcgcgtcggt cagacggttt gccgcctgct cttcccagtt atcatcttcg
    45001 atgccgtcag caaagttaga tgatttatcc tgcagataga gcaccggagc catcggctgg
    45061 ctgtcggaat cgtcatcgga agacaggtca aaggtcatgt cctgtgccgc catacgcgat
    45121 tccatctcac gtacgtcttt gctggttacg cccagttcac gggccaccat ttcgacttca
    45181 tcctgattaa accagcccag acgctgcttg gttttacgca ggttgaagaa cagtttgcgc
    45241 tgcgctttgg tggtcgcaac tttgacgata cgccagttgc gcagaacgta ttcgtggatc
    45301 tctgctttga tccagtgaac ggcgaaggag accaggcgca cacccacttc cgggttaaaa
    45361 cggcgcactg ctttcatcag gccgatgtta ccttcctgaa tcaaatccgc ctgtggcagg
    45421 ccatagcccg cataattacg agcaatatga acaacaaacc gcaggtgaga caggatcagc
    45481 gttttagctg cttccagatc gccatggtaa tgcagctttt cagccagcgc ccgctcctcg
    45541 tcagccgaca acatcggcca cgcgttagct gcccggatgt aggaatccag gttgccaact
    45601 ggggctaaag ctaaactttg cattttgtca gtcattcaaa tcctctcaat cgatatcttc
    45661 tggcgcttca gtggtagcaa caactgtgcc agagcttaag agcaacgagg ttatcattca
    45721 ctgttttatc agacagtgat tttatccaca agttcaatgc aacgctgtga ataaattacg
    45781 cacaaaatgt gacatagaga tgaaataccg ggaagagaca acggggtctc tttccctgct
    45841 acggaaccca ttgcagggaa agagtataac acgcttttat tattcaggcg taaagtggcg
    45901 taaatgttgt accgtggcaa gccacgctgc cacccagcca atcatcgagc ataccagcag
    45961 caatagcagg cattcatcga acgataaacc attgatatca aacttcgttc cgaaaacctg
    46021 cgccacttcc gcaaccgccg atgacaatcg cagcaccaga atttctgaca aaattaatga
    46081 caacaatgcg ccagaaaatc ccagcaatgc gcctccgtat aggaacgggc gcaggatgaa
    46141 accatccgtc gcaccaatca gtttctgcac gttaatggag tcacggcgag caaagatact
    46201 caggcgcaca ctgttaccga tgacgaggaa cactgctgcc accatcaaca cgccgatcat
    46261 cgcggaaacg cgcccgacca gcccggttaa cgccgccaga cgggcaaacc agctgtcatc
    46321 catccgcact tcgtcaatgc cgttgatctg ggtgatacga tcacgcagcg tattcagtga
    46381 ttccgtcccc tgaaaatcga gtttcgggat caccaccgcc actgccggaa gcgggttttc
    46441 ttccagcata tccagcgcac caccaaaacc agaccagtta cggaactcgc ccagtgcgtc
    46501 ttcacgagaa agataattca ctttctccac gccttgctcg gcctgcaact gtgccaccac
    46561 gcccgcagcg gcgtcatcgt ccagcgtttt ttgcagataa acagtgattt gcggtgacgg
    46621 ataatattgc gtcgccgcct ggttaacgtt tttgtacacc atataacaga cgctgggcag
    46681 cgtcagggag atggcgataa ccatcaccgt taaaaacgtg gcgaacggtt tgcttttcag
    46741 atcctgcaat gcgccgtgga aggcatagcg cacctgttcg ttgaaaacgt tggttttgcg
    46801 atttaccggt tttggcgagg atttcgcccg ttttggcgcg ttacgaccgc cgtcgccaga
    46861 gccgccgacc gatttacgga agcgatccag acgcccgcca aactgccgaa tatgattgat
    46921 tgcatcgcgc ttattcatgg cccacgcctc catgcaagtg accatcgctc agggtgagca
    46981 tgcgatagga acgccgcgag atcaggttga tgtcgtgcgt tgccatcaat acggttaccc
    47041 caacgcggtt aaactcttca aacagacgta aaatgccttc cgacaacgcg tcgtccaggt
    47101 taccggtcgg ttcatccgcc agcagtaccg caggcttgtt caccaccgcg cgggcaatgc
    47161 caacacgttg ttgttcaccg ccggaaagct gaataggaaa gttcttcgct ttgtccagta
    47221 gcccgacttt atccagtgcc gccgacaccc ggcgacgaat atcgtcaccg ctggcaccgg
    47281 cgataatcag cgggatcgcc acattatcgt agacagtacg gtccatcagt agatggtgat
    47341 cctggaaaat catgccaatc tggcggcgca gaaacggaac ttcacggttt ttcagacgcg
    47401 tgatgtcatg gccgctaaac cagattttcc cggcgctggg ccgctcaatc ccacagatca
    47461 gcttcaggag ggtacttttc cctgcgccgg aatgaccggt cagaaacgcc atctcacccg
    47521 gctgcatatg gaacgtaacg ccctgcagcg cctgtctccc accgagataa gccttgctga
    47581 catgttcaaa gcgaatcatt gttaatcctc tcgggcaaaa agtgcctcta taaagtcgtc
    47641 cgccttaaac ggacgcaaat cctcaatacg ttcgccgaca ccaatgtagc ggatagggat
    47701 accaaactgg tcagccaccg agaaaattac cccgcctttc gccgtgccgt ccagtttcgt
    47761 tagcgtgatg ccggttaagc caacggcttc gtggaacagt ttggcctggc ttaccgcatt
    47821 ctgcccggtg ctggcatcaa tagtcagcat aacttcatgc ggagcttcaa cgtcgagttt
    47881 cttcatcacg cggacgattt tcttcaactc ttccatcagg tgcgatttgt tttgcaggcg
    47941 tccggcggta tcggcaatca ggacgtcgat gttacgcgct ttagcggcct gaatggcgtc
    48001 gaagataaca gaagcggaat ccgcaccggt atgctgggca atcaccggaa tattgttgcg
    48061 ctgaccccag acctgaagct gttcaaccgc tgccgcacgg aaagtatcgc ctgccgccag
    48121 catcaccgat ttaccctgct gctcaaactg acgcgccagc ttaccaatcg tcgtggtttt
    48181 acccacaccg ttgacgccca ccatcaggat cacgaacggc gttttacctt caacattcag
    48241 cggctcatcg actttcgcca gaatctcgcc catctcttct ttcagcaggc catagagcgc
    48301 ctcggcatca cgaagctgct tgcgggatgc gccttccgtc agattggtga taattttacg
    48361 tgtggtttcc acgcccacat cggcgatcaa cagctgttct tccagctctt caaacagatc
    48421 atcgtcgatt tttttaccgc ggaacaggct gataaatccg gaaccgagat tttctttggt
    48481 ttttaacagg ctgcgtttca ggcgcgcgaa gaaaccttct ttggtcggtt tttcctgctc
    48541 ctgagcgatt tcttccaccg gctgctcttc ttctgccgga gaaaccacca ttaccgcctc
    48601 ttctgccgct tcggcagcca gcgcctgtgc ttccagctct tcgtcggtga tgtcttcttt
    48661 agccgcttct tcttccgccg cttcgacaat ctctagggtt tccgcttcag cctgccactc
    48721 ttctggcgaa acctcttcgg cgttgacgtc ttccggcaac ggcagctctt cacgctcgat
    48781 agccactggc tctggcgtaa cttcaggttc aatgactgac gctggcgttt cttcaactat
    48841 cgtttccggc tcagtaataa cttccggttc agcaacagct tcaggctgcg ctttttcact
    48901 ttcagcaacc tgttcagtaa cttccacaac gtcggcagca aaagtttccg cctcggcttc
    48961 agtatgcgcc tgcggctgct cttcaacggt ttgttcagaa gccttcacag gctcttgcgc
    49021 cggaacgatt tcttctacaa ctggttgttc attctgaact tctgtctctt tttccggggt
    49081 ctgctctttt tgaccaaagc ccagccagga aaaaaagcca cgtttttttt ctttcgccat
    49141 ttgcgactac actcctcgct gttgcttcat ggcacagcgt taacgctatg tacatagcag
    49201 ctaaaaaaat gatgaaatag tctatcactt aacttaattc acatcaccgc ctgcaagtat
    49261 gtgttatctg gcggattgag caatttatca tgaaaaaacc gaatcattcc ggcagcggcc
    49321 aaatccgcat tattggcggg cagtggcgag gccgtaaact cccggttcct gatagcccag
    49381 gtctgcgccc caccaccgac cgcgtacgcg aaacgttgtt taactggctg gctccggtca
    49441 ttgttgacgc ccaatgtctg gattgcttcg ccggaagcgg cgcgctgggg ctggaagcgt
    49501 tatcgcgcta cgctgcgggg gccacgttga ttgagatgga tcgtgcggtt tctcagcagt
    49561 taattaagaa tctggcgaca ctaaaagcag gcaatgcacg cgtggtgaac agcaacgcga
    49621 tgtcattcct ggcgcaaaaa ggcacaccgc ataatatcgt gtttgtcgat ccaccgttcc
    49681 gccgtggatt gttagaagag acgataaatt tactggaaga taacggctgg ctggctgacg
    49741 aagccctgat ttatgtcgaa agcgaagtcg aaaacggtct gcccactgtt ccggcgaact
    49801 ggtcattaca tcgggaaaaa gtggcgggtc aggtggctta tcggctgtat caacgcgaag
    49861 cacaaggaga aagtgatgtt gattaatatt ggacgtttgt taatgctctg cgtttgggga
    49921 tttttaatcc tcaacctggt gcatcccttc ccgcgtccgc tgaatatttt cgttaacgtg
    49981 gcgctgattt ttaccgtgct gatgcatggt atgcagctgg cgctattgaa atccactttg
    50041 ccgaaagatg gcccgcagat gaccaccgcc gaaaaggtgc ggattttcct tttcggcgtg
    50101 tttgaactgc tggtctggca gaagaaattt aaagttaaaa aataacctta ttgttcgcct
    50161 ataaagctga caaagcgcgt tcctttatag ctcagcgtac ctttatcgcc cactgtcagg
    50221 gcgtggtact gctgggcgtc taggcgaaac gtctgctcca ttcctccgct ttgcggtttg
    50281 aagcttgcct catagcgcat actggtgcct gccggagtta cttcctgctg gcgcgaacgg
    50341 cgatcgttaa tcggtttttc ccgcttgttg ctcaccacca ccagcttttg ctggagcggt
    50401 gccatatcat tatcagcttt ttcccgtcgt tgttgcataa aacgaaacga tgcggcgacg
    50461 atgattaagc caatgataac aataaagaaa agaggtggtt tactcatctt tatccctcat
    50521 cggaaaatgc ggaaataagc ataccctgcc agttatggtg ttgtcatccg tccaccctcg
    50581 ccactaaact ggaagcaaga ccgtaggcat tccgcttacg aaaaaataac gaattcaagg
    50641 aactaagatg ctttggtcgt ttatcgctgt ctgtctttcc gcatggctat ctgtggatgc
    50701 atcgtatcgt gggccaacct ggcaacgctg ggtgtttaaa ccgttaaccc ttcttctcct
    50761 gctgttgctg gcctggcaag cgccgatgtt cgacgccatt agctatctgg tgctggcggg
    50821 gctgtgtgcc tcactgctgg gcgatgcgct aaccctgttg ccacgtcaac gtctgatgta
    50881 cgccatcggc gcgtttttcc tctcgcacct gctgtacacc atctatttcg ccagtcagat
    50941 gacgctctcc ttcttctggc ctctgccgtt ggtactgttg gttctgggcg cgctgttact
    51001 ggcgattatc tggacgcgtc tggaagagta tcgttggcct atctgcacgt ttatcggcat
    51061 gacgctggtg atggtgtggc tggcaggtga actgtggttc ttccgtccga ccgctccggc
    51121 actctctgcg tttgtcggcg cttcgttgct gtttatcagt aactttgtct ggctgggtag
    51181 tcactatcgc cgccgcttcc gtgcggacaa cgcgattgct gcggcctgct actttgctgg
    51241 tcacttcctc atcgtccgct cgctgtatct ctgataaaac ttgactctgg agtcgactcc
    51301 agagtgtatc cttcggttaa tgagaaaaaa cttaaccgga ggatgccatg tcgactcctg
    51361 acaatcacgg caagaaagcc cctcaatttg ctgcgttcaa accgctaact acggtacaga
    51421 acaccaacga ctgttgctgc gatggcgcat gttccagcac gccaacgctc tctgaaagcg
    51481 tctccggcac ccgctatagc tggaaagtca gcggcatgga ctgcgccgcc tgtgcgcgca
    51541 aagtggaaaa tgccgtgcgc cagcttgcag gcgtgaatca ggtgcaggtg ttgttcgcca
    51601 ccgaaaaatt ggtagtcgat gccgacaatg acatccgtgc ccaggttgaa tctgcggtgc
    51661 aaaaagcggg ctattccctg cgcgatgaac agaccaccga cgaaccacaa gcatcacgcc
    51721 tgaaagagaa tctgccgctg attacgctaa tcgtgatgat ggcaatcagc tggggtctgg
    51781 agcagttcaa tcatccgttt ggtcaactgg cgtttatcgc gaccacgctg gttgggctgt
    51841 acccgattgc tcgtcaggca ttacggctga tcaaatctgg cagctacttc gccattgaaa
    51901 ccttaatgag cgtagccgct attggtgcgc tgtttattgg cgcaacggct gaagctgcga
    51961 tggtgttgct gctgtttttg attggtgaac gactggaagg ctgggccgcc agccgcgcgc
    52021 gtcagggcgt tagcgcgtta atggcgctga aaccggaaac cgccacgcgt ctgcgtaacg
    52081 gtgagcgtga agaggtggcg attaacagcc tgcgccctgg cgatgtgatt gaagttgcgg
    52141 caggtgggcg tttgcctgcc gacggtaaac tgctctcacc gtttgccagt tttgatgaaa
    52201 gcgccctgac cggagaatcc attccggtgg agcgcgcaac gggcgataaa gtccctgctg
    52261 gtgccaccag cgtagaccgt ctggtaacgc tggaagtgct gtcagaaccg ggtgccagcg
    52321 ccattgaccg gattctgaaa ctgatcgaag aagccgaaga gcgtcgcgcc cccattgagc
    52381 ggtttatcga ccgtttcagc cgtatctaca cgccagcgat tatggccgtc gctctgctgg
    52441 tgacgctggt gccgccgctg ctgtttgccg ccagctggca agagtggatt tataaagggc
    52501 tgacgctgct gctgattggt tgcccgtgtg cgttagttat ctccacgccc gcggcgatta
    52561 cctccgggct ggcggcagca gcgcgtcgtg gggcgttgat taaaggcggc gcggcgctgg
    52621 agcagctggg tcgtgttacc caggtggcgt ttgataaaac cggtacgctg accgtcggta
    52681 aaccgcgcgt taccgcgatt catccggcaa cgggtattag tgaatctgaa ctgctgacac
    52741 tggcggcggc ggtcgagcaa ggcgcgacgc atccactggc acaagccatt gtgcgcgaag
    52801 cacaggttgc tgaactcgcc attcccaccg cccaatcaca gcgggcgctg gtcgggtctg
    52861 gcattgaagc gcaggttaac ggtgagcgcg tgttgatatg cgctgccgga aaacatcctg
    52921 ctgatgcatt tgctggtttg attaatgaac tggaaagcgc cgggcaaacg gtagtgctgg
    52981 tagtacgtaa tgatgacgtg ctgggtgtca ttgcgttgca ggataccctg cgcgccgatg
    53041 ctgcaactgc tatcagtgaa ctgaacgcgc tgggggttaa aggagtgatc ctcaccggcg
    53101 ataatccacg cgccgcggcg gcaattgccg gggagttggg gctggaattt aaagcgggcc
    53161 tgttgccgga agataaagtc aaagcggtga ccgagctgaa tcaacatgcg ccgctggcga
    53221 tggtcggtga tggtattaac gacgcgccag cgatgaaagc tgccgccatc gggattgcaa
    53281 tgggcagtgg cacagacgtg gcgctggaaa ccgccgatgc agcattaacc cataaccacc
    53341 tgcgcggtct ggtgcaaatg attgaactgg cacgcgccac tcacgccaat atccgccaga
    53401 acatcaccat tgcgctgggg ctgaaaggcg ttttcctcgt caccacactg ttaggaatga
    53461 ccgggctatg gctggcggtg ctggcagata cgggtgcgac ggtgctggtg acggcgaatg
    53521 cgttaagatt gttgcgcagg aagtaaaagt gttgcctgat agcctctggc tatcaggctt
    53581 agtgctgagg cgggaacggc ggcatagtca ggaaagcata ataaagcgct aacatcagaa
    53641 tgacggagat aacacagcat gcagcaacca ggcgggatgc tatggatggc cttttgaaca
    53701 tcaaccacag ggcataagca agcccgacac cgttaagtaa aggtaaaaaa atcattagcc
    53761 agagccaacc ccaggcaagt acccccagcc atccccccac gacgataaaa acagggatca
    53821 gccaattggg ggtgaagatc gcgagcaatc ccagcaatgc cagacaaact ggaattaaat
    53881 aatccatttg tgcattccat tacgtttgcg tgccggatgc ggcgtaaacg ccttatctgg
    53941 cctacacagg gcatcgaact cgtaggcatg ataagacgcg acagcgtcgc atcaggcatt
    54001 gcgcaccgta tcgccagatg cagcttgaac tttttatccg tcctacaaac cggttgcaag
    54061 ccgcaaaatt gagcgcgatc ggtccgctag cctgatccac cttaccccga tttgcgaatc
    54121 aaataacgat aaggcagtcc atccgtctct ttcgcaacca gttcgtgttc cataaaggta
    54181 caaaacccag gaatatcgcg tgtcgtcgcc ggatcgtcag caataatcag caacgtttca
    54241 cctggctgca tattgcgcac tgttttgcgc accatcatta ctggttccgg gcagcgcagg
    54301 ccaagtgcgt cgagtgtgtg gtcagggctg gaaaagagat cggtcatttt cttctcatca
    54361 cataaaaaaa cggcgctagt ttacgccctg tgagtccgta agcaaaccag gttaacgatt
    54421 gcgtgaaaat taaccattgc attgttaacg taaagcagta tcatgcggcg gctcgaaaaa
    54481 agggtcagca cgttattatg ttaaggtaac agacgtgtcg tacgtattgg gttccctcac
    54541 cccaatggtt aatcaaaaag gtacaatatg aacgttttct cgcaaactca acgctataag
    54601 gcgttgttct ggttatcgtt atttcatctt ctggtgatca cctccagtaa ctatctggtt
    54661 cagcttcccg tatccatttt tggtttccat accacctggg gcgcgtttag ctttccgttt
    54721 attttccttg ctaccgacct gactgtgcgt atttttggcg caccgctggc ccgtcgtatc
    54781 atctttgcgg tgatgatccc ggcgttactg atctcctacg tcatctcgtc gctgttctat
    54841 atgggttcct ggcagggatt cggcgcgctc gcccacttca acctgtttgt cgcccgtatc
    54901 gccaccgcca gtttcatggc ctacgcgctg gggcaaatcc tcgacgtgca cgtttttaac
    54961 cgcctgcgtc agagtcgtcg ctggtggctg gcaccgacgg cttccaccct gtttggtaac
    55021 gtcagcgaca cgctggcctt cttcttcatt gccttctggc gcagcccaga tgcctttatg
    55081 gctgaacact ggatggaaat cgcgctggtc gattactgtt tcaaagtgtt aatcagtatc
    55141 gttttcttcc tgccaatgta tggcgtatta ctcaatatgc tgttgaaaag actggcagat
    55201 aaatccgaaa tcaacgcttt gcaggcgagt taaaggttcg ttatcagagt tgtgataaga
    55261 tggatgaatg agccgttatg gccgtttatc gaaaggaaga agtcaatgcg caatctggtt
    55321 aaatatgtcg gaattggcct gctggttatg gggcttgcgg cctgtgatga taaagacact
    55381 aacgctacgg cgcagggttc ggtcgcggaa agtaacgcta ccgggaatcc cgtcaacctg
    55441 cttgatggca agttaagttt ctcgctgcca gcggatatga ccgaccagag cggtaagctg
    55501 ggaacgcagg ctaataacat gcatgtctgg tccgacgcca ccgggcaaaa agcagtcatc
    55561 gtcatcatgg gcgatgatcc gaaagaagat ctggcggtgc tggcgaagcg cctggaagat
    55621 cagcaacgta gccgcgatcc gcagctgcaa gtggtaacca ataaagccat tgagctgaaa
    55681 ggtcacaaaa tgcagcagtt agacagcatt atctccgcga aaggccagac ggcgtactct
    55741 tccgttattc tgggtaacgt gggtaatcaa ctactgacca tgcaaattac actgcccgct
    55801 gacgatcagc aaaaagcaca gaccaccgca gaaaacatca ttaatacgct ggttattcag
    55861 taagttttaa gatgatgaga tggcctcagg tacttccacc ggaggccgtt tttttaatcg
    55921 ccacgtcagt aataacgcga ttgcgaccag tcccgccgcc gccagataaa tcaccgggac
    55981 gcccgcccaa ctcatcacca gcccagccag tggtccggtc acgccaagcg ataaatccat
    56041 aaatacggtg taagttgcca gcgccgcccc ctgattttgc tgcggaaccg cttttaccgc
    56101 cactacgccc aatgccggga acaccagcga aaaccccgcc cccgcgagta agacgccgat
    56161 tttcgccatc cacggcatag tcgccacgcc aaccagtagc aggccgatta tctcaacgct
    56221 aaagcaaatc atcgccacgt ttaagccgcc gatacggtta atgccgttag ggaataacaa
    56281 acgcgtaccg acaaacgcac agctaaacag cgtcagcgcg aaagccgcac cgtcccaacc
    56341 tttagcgtca taaaacagcg tgataaaggt ggcgataacg ccaaatccgg cggaagccag
    56401 tgccagcgcc ataccgtaca gccagacgcg cccaagcacc gcacgaaacg gcagcggttt
    56461 gcctttactg gcttttacca tcggacgcgg gatcgccaac aaaatggcca ccagcgccac
    56521 gcccataatg attaacgcta acgcctgcaa tccgccccag tgataaaaca cgacacctaa
    56581 cggcgcaccc atcgccatcg ccccgtaagt gacaataccg ttccacgaaa tcacccgccc
    56641 gatatgtagc gaaccaacca cgccaacacc ccacagggtc gatcccgttc cggcaaaact
    56701 ttgcccaatc ccaaggatta cgcgccccag gcacagtaat aacaggctga tgacgggcag
    56761 actggcggtt aatcccgccg tcagatatcc cagaccgctc aaaaagcagc cgcataaacc
    56821 gaagacgaca atctttttgg gtcccagcaa atcggcgtaa cgtccggcat gaggacggct
    56881 cagcaaggtg gcgaaatatt gcaggctgat aaccaaccct gcccagaagg cactaaatcc
    56941 catcacatca tggacatagc ccggtaatac agcgagcggc aacccgatgg tgaggtagct
    57001 ggcgaagtta aacatgacaa tggagacaat gcgcaaattc aggcgcaatc cgtttagcgc
    57061 gggttcggct acgggttcgg gcatgaggat caccacattt ttacaacagt gtttcatttt
    57121 taccacgtgc tgacgtgaaa atcagcagta agaatcagaa tattgctggc gtgactcccg
    57181 ctacacttaa tacaaaaagt cacaaggaag ccccaatgga aacccctcaa cccgataaaa
    57241 cgggcatgca cattctgctc aagctggcct cgctggtggt gatcctcgcg ggaattcacg
    57301 cagcggcaga tatcattgtg cagctgttac tggcgctgtt ttttgccatc gtcctcaacc
    57361 cgctcgtcac ctggtttatt cgtcggggag tacaacgccc cgttgccatt acgattgtgg
    57421 tggtggtgat gctgatcgca ctaaccgcgc tggtcggcgt actggcggca tcgtttaacg
    57481 aatttatctc tatgctgccg aagtttaata aggagctgac gcgcaaactt tttaaattgc
    57541 aggagatgtt gccttttctt aatttgcata tgtcgccgga gcgaatgctg cagcggatgg
    57601 actcggaaaa aatggttacc ttcaccacgg cgctaatgac cgggctttcc ggggcaatgg
    57661 cgagcgtgct tttgctggtg atgaccgtag ttttcatgct gtttgaagtg cgccacgtcc
    57721 cttacaaaat gcgttttgcg ttgaataatc cacagattca catcgccgga ctacaccgcg
    57781 ccttaaaagg tgtttcgcac tatcttgcat tgaagacact gctaagttta tggacaggcg
    57841 taatcgtctg gctggggctg gcgctaatgg gcgtacagtt tgcgctgatg tgggcagtac
    57901 tggcgttttt gctcaactac gtgcccaata tcggcgcggt aatttccgcc gtaccgccaa
    57961 tgattcaggt gctgctgttt aatggcgttt acgaatgtat tctggtcggc gcattgtttt
    58021 tagtggtcca tatggtcatc ggcaatattt tagaaccacg gatgatgggc catcgcctgg
    58081 ggatgtccac catggtggta tttctttcat tgttaatttg gggatggctg ctcggcccgg
    58141 tagggatgct actttcggta ccattaacca gcgtgtgtaa aatctggatg gaaaccacca
    58201 aaggcggtag caaactggcg attttactgg gaccgggcag accgaaaagt cgattaccgg
    58261 gatgaggcga caagtgttac gatacgcact ttcattttcc attaaacgtt ggccctgata
    58321 tgtatcggat agttctgggg aaagtttcga ccttaagcgc agctcctctg ccaccgggtt
    58381 tacgcgagca agcaccgcaa ggtccacgac gcgaacgctg gctggcgggg cgtgcattgc
    58441 tttcgcacac gctttccccg ctaccggaga tcatctatgg cgaacaaggc aaacctgcat
    58501 tcgcgccgga aacgccgcta tggttcaact taagccatag cggtgacgat atcgccctgc
    58561 tgttgagcga tgaaggcgaa gtcggctgcg atatcgaagt gattcgcccg cgcgccaact
    58621 ggcgctggct ggcgaacgcc gtattcagcc ttggggaaca tgccgagatg gacgccgtgc
    58681 atcctgatca gcaactggaa atgttctggc gcatctggac gcgcaaagaa gccatcgtta
    58741 aacagcgtgg cggcagcgcc tggcaaatcg tcagcgtaga cagcacctat aactcctcgc
    58801 tgtcagtcag ccattgtcag ctagaaaatt taagcctggc gatctgcacc cctaccccct
    58861 ttacgctcac cgccgacagt gtgcaatgga tcgattcagt taactgatcc gcccacccga
    58921 ctgcccatct attgatccag aacaggtaat cagtatgacg aatacttaaa atcgtcatac
    58981 ttatttccgc catctatttt aatccattgg ggttaccatg ttctctacac tccgccgcac
    59041 tctatttgcg ctgctggctt gtgcgtcttt tatcgtccat gccgctgcac cagatgaaat
    59101 caccaccgcc tggccggtga atgtcgggcc actaaacccg cacctttaca cgcctaacca
    59161 aatgttcgcc cagagcatgg tttatgaacc actggtgaaa tatcaggcag acggttcggt
    59221 gatcccgtgg ctggcaaaaa gctggaccca ttcagaagat ggcaaaacct ggaccttcac
    59281 cctgcgtgat gatgtgaaat tctctaacgg tgaaccgttt gatgcagagg ccgcggcaga
    59341 aaacttccgc gctgtgctcg acaaccgtca acgtcacgcc tggctggagc tggcaaacca
    59401 gattgttgat gtcaaagcac tcagcaaaaa cgagctgcaa atcaccctga aaagcgccta
    59461 ctatcctttc ctgcaagaac tggccctgcc gcgccctttc cgctttatcg ccccctcgca
    59521 gtttaaaaac catgaaacca tgaatgggat taaagcgccg attggcaccg ggccgtgggt
    59581 tttgcaggaa tcgaaactga atcagtacga tgtcttcgtc cgtaacgaaa actactgggg
    59641 cgaaaagccg gcgattaaga agatcacttt taacgtcatc ccagacccga ctacccgcgc
    59701 agtagcgttt gaaaccggcg atatcgatct actatacggt aatgaagggc tattaccgct
    59761 cgataccttc gcccgcttta gccagaactc ggcttaccac acccaactgt cacagccgat
    59821 cgaaaccgtg atgctggcgc tcaatactgc caaagccccc accaacgagc tggccgtacg
    59881 tgaagctctc aattacgcgg taaacaaaaa atcgctgatc gataacgcgt tgtatggcac
    59941 ccagcaggtc gccgacaccc tgtttgcccc ttccgtgccc tatgccaatc tcggcctgaa
    60001 accgcgccag tacgatccgc agaaagcgaa agcgttgctg gaacaagcag gctggacgct
    60061 gcccgcaggc aaagacatcc gcgagaaaaa tggtcagccg ctgcgcattg aactgtcgtt
    60121 catcggcacc gatgcgttaa gcaaatcaat ggccgaaatc attcaggctg atatgcgcca
    60181 gattggcgcg gatgtcacgc tgatcggcga agaagagagc agtatctatg ctcgtcagcg
    60241 cgacggtcgt tttggcatga ttttccaccg tacctggggc gcgccatacg atccacacgc
    60301 cttcctgagt tcaatgcgcg taccgtcgca cgctgacttc caggcacagc aaggtttagc
    60361 cgacaagcca ctgattgata aagagattgg cgaagtgctg gcgacacacg acgaaacaca
    60421 acgtcaggca ctgtatcgcg acattctgac ccgtctgcat aacgaggcgg tttatctgcc
    60481 catcagctat atctcaatga tggtggtttc aaaaccggaa ctgggtaaca tcccctacgc
    60541 gccgatcgcc accgaaatcc cgttcgaaca gattaaaccg gtgaaacctt aatgttgcgt
    60601 tatgtattac gccgctttct gctgctgatc ccgatggtgc tcgccgcctc ggtgatcatt
    60661 tttctgatgc tgcgcctcgg caccggcgac ccggcgctcg attatctgcg tctgtctaac
    60721 ctgccgccga cgccggaaat gctggcttct acccgcacca tgctggggct ggatcagccg
    60781 ttgtacgtgc agtacggaac ctggttgtgg aaagcgctgc atcttgactt tggtatctca
    60841 ttcgccagcc aacgcccggt gctggacgat atgctgacct tcctgcccgc cacgctggag
    60901 ctggcgggtg cggcgctggt attaattctg ctcacttccg tgccgctcgg catctgggcg
    60961 gcgcgccatc gcgaccgcct gccggatttc gccgtgcgtt ttatcgcgtt tcttggcgtg
    61021 tcgatgccta acttctggtt ggcgttttta ctggtgatgg cattttcagt gtatctgcaa
    61081 tggctgcccg cgatgggtta cggcggctgg cagcacatca ttttgcctgc ggtttccatc
    61141 gcttttatgt cgctggcgat taacgcgcgt ctgctgcgcg ccagtatgct ggacgtcgcc
    61201 ggtcagcgtc atgtgacctg ggcgcgactg cgcggcctga acgacaaaca gaccgaacgc
    61261 cgccacatcc tgcgcaatgc ctcgctgccg atgatcaccg ccgtggggat gcacattggc
    61321 gaactgattg gcgggacgat gattatcgaa aacatctttg cctggccggg cgtcgggcgc
    61381 tatgcggtgt cggcgatttt caaccgtgac tatccggtga tccagtgctt tacgctgatg
    61441 atggtggtgg tttttgtggt ctgtaatttg attgtcgatt tgctcaacgc cgcgctggac
    61501 ccacgtattc gtcgtcatga aggagcccac gcgtgaactt tttcctctcc tcccgctggt
    61561 cggttcgcct ggcgctgatc attatcgccc tgctggcact gattgcgctc accagccagt
    61621 ggtggctgcc gtatgaccca caggcgattg atctgccgtc gcgcctgctt tcaccggatg
    61681 cgcagcactg gctgggtacc gatcatttag ggcgcgatat tttctcgcgg ctgatggcgg
    61741 cgacccgcgt gtcgctcggc tcggtaatgg cctgcctgct gctggtgctg acattagggc
    61801 tggttattgg cggcagcgcc ggactgattg gcgggcgcgt tgatcaggcc accatgcgcg
    61861 tcgcggatat gtttatgacc ttcccgacct cgattctgtc gttctttatg gttggcgtgc
    61921 tcggcaccgg gctgaccaac gtaattatcg ccatcgccct gtcgcactgg gcgtggtatg
    61981 cacgcatggt gcgcagcctg gtgatttcgc tacgccagcg cgagtttgta ctggcatcac
    62041 ggctttctgg cgcgggccat gtgcgagtgt ttgtagatca tctggcaggt gcggtgatcc
    62101 cttcgctgct ggtgctggca acgctggata tcggccatat gatgctacac gtcgcgggga
    62161 tgtccttcct cggcctcggc gtgaccgcgc ctaccgccga atggggcgtg atgattaacg
    62221 acgcgcgcca gtatatctgg acccagccgc tgcaaatgtt ctggccgggg ctggcgctgt
    62281 ttatcagcgt gatggccttt aacctggtgg gtgacgcact gcgcgatcat ctggaccctc
    62341 atctggtgac ggagcacgca cactaatgcc gcaacagatt gaactacgta atatcgcgct
    62401 acaggccgcg cagccgctgg tgcacggcgt atcgttaacc cttaaacgcg ggcgtgtgct
    62461 ggcgttagtc ggcggtagcg gcagcgggaa atcactgacc tgcgccgcga cgctgggcat
    62521 tctgcccgca ggcgttcgcc agacggcggg ggaaatttta gccgatggca aaccggtttc
    62581 tccctgtgcc ctgcgcggca tcaaaattgc caccatcatg cagaacccgc gcagtgcctt
    62641 taatccgctt cacaccatgc acacccacgc gcgggaaacc tgcctggcgc tggggaaacc
    62701 cgccgatgac gctacgctta ccgctgccat agaagcggtg gggctggaaa acgccgcacg
    62761 cgtgctgaag ctgtacccgt ttgagatgag cggcggcatg ttgcagcgca tgatgattgc
    62821 gatggcggtg ttgtgtgaat cgccgtttat catcgccgat gaaccgacca ccgatctcga
    62881 cgtggtagca caggcgcgca ttctcgatct gctggaaagc attatgcaaa aacaagcacc
    62941 gggaatgctg ctggtgaccc atgatatggg cgttgtggcg cgtctggcgg atgacgtggc
    63001 ggtgatgtca cacggtaaaa ttgtcgaaca gggcgatgta gaaacgctgt ttaatgcccc
    63061 caaacatgcg gtaacgcgca gcctggtttc cgctcatctc gccctctacg gtatggagct
    63121 ggcatcatga ctttacttaa cgtctccgac ctttcccatc actatgcgca cggtggattt
    63181 agcggaaaac atcaacacca ggcggtgctg aataacgttt ccctgaccct gaaaagcggc
    63241 gaaaccgtcg ctctgctggg gcgcagcggc tgcggaaaaa gcactctcgc gcggctgctg
    63301 gtaggcttag agtctccctc acaagggaat attagctggc gcggcgaacc gttggcaaag
    63361 ctcaatcgcg cccagcgtaa agcgttccgc cgcgatattc agatggtatt tcaggactcc
    63421 atcagcgccg tgaatccgcg caaaaccgtg cgcgagatcc tgcgagaacc gatgcgccac
    63481 ctgctatcgc tgaaaaaagc cgaacaactg gcgcgcgcca gcgaaatgct gaaggcggtc
    63541 gatctcgatg acagcgttct agacaaacgc ccaccgcagt taagcggcgg tcagctccag
    63601 cgcgtctgcc tggctcgcgc gctggcggtc gaaccgaaac tactgattct ggatgaagcc
    63661 gtttctaacc ttgatctcgt gttacaggcg ggcgtcattc gcctgctgaa aaagctacaa
    63721 caacagtttg gcactgcctg cctgttcatc actcatgact tacgcctggt ggaacgcttt
    63781 tgccagcgag tgatggtgat ggacaacgga caaatcgtcg aaacccaggc ggtgggagat
    63841 aaattaacct tttcctctga cgccggacgt gtgctacaaa acgcggtatt acccgcattc
    63901 cccgtgcgcc gtcgcaccac agaaaaggtt taacgcaaat gcaacgaatc accatcacgc
    63961 ttgatgacga tttactggag acgctggaca gcctgagcca gcgtcgtggc tataacaacc
    64021 gttccgaagc tatccgcgat attctgcgta gcgccctggc gcaagaggcc acccagcagc
    64081 acggcacaca aggtttcgca gtgctctcct atgtgtatga acacgaaaaa cgcgacttag
    64141 ccagccgtat tgtctccacc cagcaccatc accacgatct ctcggtcgcc acgctgcatg
    64201 tgcacattaa tcacgacgac tgtctggaaa tcgccgtgct gaaaggtgac atgggcgacg
    64261 tgcagcattt tgccgatgac gttatcgccc agcgcggcgt gcggcacggg catttgcagt
    64321 gcttgccgaa ggaagattaa cacctaccca cgcgccgtta tttcctgacg gcgcagtaaa
    64381 ttcctgccaa atatcgactc acgtcacatt tttttgatca cgatttggtc cctcaaaatg
    64441 tgagacgctt cgatcctctt tttctcccgt tcacctacta tacgcttgaa gttgtccatg
    64501 attgtccata gacaactgta aatgttcagg tgcgcgccat gaaatcatta agtaagtcgt
    64561 cacaaatacc gctctatcaa caagtggtgg agtggataag agaaagtatt tataccgggg
    64621 atctggtgga agacgatcgc attccttcgg aataccagat tatggatatg ctggaagtga
    64681 gtcggggaac cgttaaaaaa gcggtcgccc aactggtaaa agaaggcgtg ttgattcagg
    64741 tccaggggaa gggaactttt gtcaaaaaag agaacgtggc atatccgtta ggtgaaggat
    64801 tattgtcatt cgcggaatcg ctggaaagcc agaaaataca ttttaccact gaagttatta
    64861 cgtcacggat tgaaccggct aatcgttatg tggcagagaa attaagaata acgcccggtc
    64921 aggatattct ttaccttgag cgtttacgtt caattggtga tgaaaaagcg atgctgatag
    64981 agaaccgtat caatattgag ctatgccccg gcatcgtgga aatcgatttt aatcaacaca
    65041 atttatttcc aacaatagaa agtttgtcgc aaagaaaaat tcgttactcg gaaagtcgct
    65101 atgccgcgcg attaattggt aatgaacgcg gtcatttttt agatatcagt gaagatgcac
    65161 ccgttttgca tctggagcag ttagtctttt tctcccgaga gttacccgtt gagtttggca
    65221 acgtctggtt aaaaggaaat aaatattatc tgggcaccgt gctgcaacgg cgggaagtga
    65281 gttaaataca cttttttatg aggaataaaa tgcaaggcat acagtttcag gaaaattata
    65341 ttcaacgact tccggcaggg ttaagcgttg aacagatcat ccgtcagtta gcgcaaccgt
    65401 tggttgctgc tgagctggtg gtccctgatt ttgccaatca cgtactgaaa cgtgaggcga
    65461 cgtatccaac ggggctgcct accgaaccac cgtgcgtcgc cattccgcac acggaccata
    65521 aacatgtccg gcacaatgca attgccgtcg gtattttgcc agagccggta gagtttgccg
    65581 atatgggcgg cgaccctgat cccgtgcctg tcagggtgat ttttttgctc gccttaagcg
    65641 aaagtaacaa acaattaaat gccctgggct ggattatgga gatgattcag gatacgccct
    65701 ttatgcaagc cttgcttacg atggaaacaa cagaaataca cacagtaatc ttaaacaaaa
    65761 tgaatgaacg aggtgaaata tgagtcaaac cattttattt gtctgcgcca caggtattgc
    65821 cacatccacg gcggtaacag aaaaagtcat ggagtattgc aaagagcacg gccttaacgt
    65881 caattattcc caaaccaacg ttgcctcttt acccggtaat accgacggcg tagcgctggt
    65941 tgtttcaacg actaaagtgc cttatgaact cgacgtgccg gtggttaacg gcttacccat
    66001 tattaccggt attggcgaag agaaagtgct ggcgcaaatt gtttcgattc ttaaaaagta
    66061 attaataatt aattccaacc cactccatca ggcgctaacg cgaggtgcgc tctcgcctga
    66121 aaaagaggtt tattatgaac gatatcgcgc ataccctcta taatattgtg caatatatat
    66181 tgggatttgg ccccacggta atgttgccgt tggtgttatt tattcttgct ctctgtttta
    66241 aagtaaaacc cgctaaagcc ttacgctcgt cattaacggt cggcattggt tttgtcggta
    66301 tttatgccat tttcgatatt cttaccagca atgtcgggcc agcggcccag gcgatggttg
    66361 aacgcaccgg aattaattta ccggtggtgg atttaggctg gccgccgctt tctgccatta
    66421 cctgggggtc gccaattgcc ccgtttgtta ttcccctgac cattctgatt aatgtcgcaa
    66481 tgctggcgtt gaataaaacc cgcaccgttg atgtagatat gtggaactac tggcattttg
    66541 cccttgccgg tacgctggtt tattacagca ccggcagcct gttctttggt ttgctggcgg
    66601 ccgcgattgc cgcagtggtg gtgctaaaac tcgccgactg gtctgcgcca ctggtacaaa
    66661 aatactttgg cctggaaggg atctcattgc cgacgctctc ttcggtggtg ttcttcccgg
    66721 tcggtctgct ggtcgacaaa atcatcgacc atatccctgg cctcaatcgt attcatatcg
    66781 acccggaaac cgtacagaaa aagtttggca tcttcggcga accgatgatg gttggcacta
    66841 ttctgggcat tctgctcggc gtaattgccg gatacgattt caaaaaagtc ttgctgcttg
    66901 gcatcagcat tggcggtgtg atgttcatcc tgccacgcat ggtacgcatc ctgatggaag
    66961 gtttattacc gctgtctgaa gccattaaaa agtatctcaa tgccaaatac cctgaccgtg
    67021 acgatctcta tatcggcctg gatatcgccg ttgccgtagg taacccggcg attatctcca
    67081 ccgccctgct gctgacgcca atctcggtct ttatcgcgtt tgtcctccca ggtaatgaag
    67141 tcctgccgct tggtgacctt gccaacctgg cggtaatggc gtcgatgatt gctttagcca
    67201 gccgtggcaa tattttccgc accgttctgg cggcgatccc ggtgattatt gccgacctgt
    67261 ggattgccac taaaatcgcg ccgtttatta ccggaatggc gaaagacgtt aacttcaaat
    67321 ttgccgaagg ctccagcggc caggtttcca gtttccttga tggcggtaac ccgttccgct
    67381 tctggctgct ggaaatcttc aacggcaatc tcatcgccat tggtctggtg ccggttatcg
    67441 ccctggtact gtatggcatt ttccgaatga cgcggagcac ggtttatgcc tgataacagc
    67501 gcagctatcg ttatcgatat tggcaccacc aattgcaaag tcacctgctt ttcctgcctg
    67561 gatgcaacga cgttgggcgc gcataaattc gtgacggcaa aacagatctc cccacagggc
    67621 gatgtcgatt tcgatatcga cgccctctgg caggaggtcc gccaggcaat agcgcaactg
    67681 agcgccgctt cgccgctgcc ggtcaggcgg ataagcattg ccagttttgg cgagtcaggg
    67741 gtgtttcttg acgagcatgg cgagatcctg acgccaatgc tggcatggta tgaccgccgc
    67801 ggtgaagagt acctggcaac gcttagtgag gcagacagtg cggcgcttta tgacatctgc
    67861 gggctaccgc tgcacagcaa ttactctgcc ttcaaaatgc gttggttgct ggagcattat
    67921 ccactgcgta atcgccgcgg cctgcgttgg ctacatgcgc cggaagtact gctctggcgg
    67981 ctgactggcg aacagcgtac ggatatcacc ttagccagcc gtacgctgtg tctggacgtg
    68041 cgtaaaggcg aatggtcagc gaaagcggcg gcgttgttac acgttccctg ttcagcattt
    68101 gcgccattgg tgcagccagg cgagcacgcc ggatgggtca gcgagtcgct ctgcaaaacg
    68161 cttgggttct tgcaaccggt cagcgtgacg ctggccgggc atgaccatat ggtgggtgcg
    68221 cgggcgttgc agatgacgcc gggcgatatc cttaactcga cgggcaccac agaaggcatt
    68281 ctgcaactgg atacacaacc gacgctggat gaacatgcca aacgtgacaa gctggcaaac
    68341 ggctgttatt cgcttgcaaa ccagttcacc ctgtttgcgt cgctgccggt gggcggtttc
    68401 gctctggagt ggctgcgcaa cacgttccgg ctaaccgatg aggagatcgc cgcatcactt
    68461 actcgcggac atgcggatta cctggcgggg aactggtcgc tcgatgacat tcccgttttt
    68521 attccgcatc ttcgcggttc gggttcgccc tataaaaatc gccatacccg aggattattt
    68581 tatgggcttg gcgatacgtt aaatatcgat atgttaattg ccagcgtctc actgggatta
    68641 accatggaat ttgccaactg cttcgcctgt tttaatgtgc ccggcaccag cgcgttaaaa
    68701 gtgataggtc cggcaacccg taatcccctt tggctgcaat taaaggcgga tattttacag
    68761 cgtccggttg aagcaattgc atttaacgag gcggtttctg tcggagcatt attaaccgcc
    68821 gcaccggata ttccaccgcc gcaagtcacc atagcccaac gtttgttacc gaatcgggcg
    68881 agataccatc aattacagcg ttatcagcac aaatggaaaa gctggtatca gttgaaatta
    68941 cagcaagaag gcgtgatgcc attacatcat cgggaggagc actatgttga gtaaaaccgt
    69001 ggaggtcaga aacagtactg gcctgcatgc acgtccggcg gcctgtctgg cgaaagccgc
    69061 caaaaaatat agctgtaaag tgacgctgca ttatgaaggt aatgatatta atgccaccag
    69121 catgatgaat attatgcgcg ccggaataaa aggcggaaaa acggtggaaa tacgctgcga
    69181 aggtgatgat gaaaacgagg caatacaaac tctcactacc ttatttcgcg accgcttcgg
    69241 tgaagctgaa taattaactg atattaaaag atgaggtttt caatatgcca ttagttaatg
    69301 gacggatact gctcgaccgc attcaggaaa aacgggtatt agcgggcgca tttaatacca
    69361 ccaatctgga gacaacgatt tcaattttaa acgccattga gcgttcagga ttacccaact
    69421 tcattcagat tgcgcccacc aatgcacaac tgtcgggata cgattacatt tatgaaatcg
    69481 tgaaacgtca cgctgataaa atggatgttc cggtcagtct tcatcttgat cacgggaaaa
    69541 ctcaggagga tgttaaacag gcggtgcgcg cgggctttac ctcggtcatg attgacggtg
    69601 ccgcattctc ttttgaagag aatatcgctt tcacccagga agctgtcgat ttctgtaaat
    69661 cctatggcgt accggttgaa gcggagctgg gggcgattct cggcaaagaa gacgatcacg
    69721 tcagcgaagc cgattgcaaa acggaaccgg aaaaggtgaa gacctttgtc gaacgcaccg
    69781 gttgcgacat gctggcagtt tctattggta acgttcatgg cctggacgat ataccgcgga
    69841 tcgatatccc cctgctgaag cgcattgccg aagtcagccc ggtgccgctg gttattcacg
    69901 gcggctccgg tatcgccccg gagatattac gcagtttcgt taattatcgc gtcgctaaag
    69961 tgaatatcgc cagcgatctg cgcaaagcgt ttatcactgc cgtcggcaaa gcgtatgtga
    70021 ataatcacaa cgaggcgaat ctggcacgag tgatggcatc agcaaaaaat gccgtcgagg
    70081 aagatgtgta tagcaaaatc ctgatgatga atgaggggca tcgattgatt agaaaggcat
    70141 cttaagagcc acaaaaatat agcggcgcaa cagaaattgt tgggccgcta taagttataa
    70201 atgattcaaa cgctcattta gcgtttcaat aatataagta tttacagaga cttgttgctg
    70261 cgcagccgca ttattaaggc gttcactcag cgattctgga tagcgtaatg taaaggtctt
    70321 aatcttttct gtacgcgcat acggctcaat acccgcagct ttacaatcct caagatactc
    70381 tcgcagggaa agctcccctt ctttttgcag cccctgaatg ctgtcagaga caaaatcaca
    70441 ataacctgac aagcccagga atttgccacg aaatgcattc agttctggca cgtaagtgat
    70501 aacagcaggt tgcccggcaa tctccataga gtttggcgtc ttcagtttga tcatggtttt
    70561 acccctatac ttagtaacca gtcacgcacg ctttctacag cgcctttatc ggtatctggc
    70621 gacggatgag gccgatggaa acacgcaacg ctcatattta gtatgaactt acaacgcgaa
    70681 cctcttcctt ccttaatttc tccgcctaat gctttaacca gtgactctat atcagaccac
    70741 ttaatccctt gaggaacagg agatttaaat atttgatcca atgtattttt tgtttcttcc
    70801 gtaaagcctg taccagggtt ctcatggcat gtccttatga cttcacttta tgacatcaat
    70861 agtatgacat cacaatatga agtcaagttt cccttcctta cgccattgtc ccaatcgtct
    70921 tcctgaatcg cagcagcgca atggtaaaaa acgcgccgcc aattgccatc agcgtcagga
    70981 actgcggcca gacgatttcg aatccggcac cacggtagag aatggcctgc gcgaggctga
    71041 caaagtgtgt cgtcggcatg gtcaacataa tgtcctgcac catctgcggc atactttcgc
    71101 gcggcgtgga gccaccggaa agcatttgca gcggcagcag caccagaatc accagcagcc
    71161 ccagttgcgg cattgaacgc gctatcgtcc ccataaaaat gccgattgac gtggtggcaa
    71221 acagactgag cgccacgccc agcataaaca gcgggatcga gccttcaatc ggtacaccca
    71281 gtacaccttt caccatcagc accagcgata atcccgacac caccagcacc accagcccca
    71341 tcgaccagac ctttgccatc atgatctcaa acggcgttat cggcatcacc agtaagtgtt
    71401 ccaccgtgcc gtgttcacgc tcgcggatca gtgccgatcc ggtcaacaca atcgccagca
    71461 tggtaatgtt gttgatgatc gccatcaccc cgccaaacca cgcgggatcg aggttcgggt
    71521 taaagcgcat ccgggtttcc agcgatacca acggttcgct gttatcacgg tagcgcgcga
    71581 caaagctgtt cacttcaccg ttgataatat tctggatata cccgttgccg gtaaatgcct
    71641 ggctcatgcg cgtggcatcg acgttcacct gaatatctgg ctggcgtccg gcgaggacat
    71701 cacgctgaaa attaggcgga atatttatcg cgaaggtata gcgtccggcg tccagcccgg
    71761 catccatctc atcggcggtg atcatctccg gtggcaaaaa ccacgggcga tagaagctgt
    71821 taacgatcct gttcgataac tgcgattgat ccatatcggc aatggcgatc ggcgcgaggt
    71881 tcaacgatcc tggcgtaacg gtcgctgacg aatacaccga caccgtaaac gagaagacaa
    71941 tcagcgtcag catcgcttta tcaccgagca aactgcgcaa ctctttgata cccagattaa
    72001 aaatattgcg taaatggcgc atcatccctc ctgttttttc agcagcagga tgcttaagcc
    72061 catcaccagc gggatggcta tcagcaacgg gataaaaagt tgccacaaat cagtcaaatc
    72121 cagcgccttc gagaacgtcc cgcgggcaat agtcagaaaa tgactggtcg ggtaaacctc
    72181 gccgatccaa cgtcctggac cttccagcga agctaccgga tcgatcatcc cggaaaactg
    72241 cgtcgccgga atcaacgtga tgatcgcagt gccaaaaatg gcggcaatct ggcttttcat
    72301 aaaggtggag atcagcagcc ccattccggt ggcaatgatg atatacagca gcgccgccag
    72361 ggtgagcgtc aggaaactgc ctttatgcgg cacgccaaac acaaacaccg acagggcgca
    72421 gagcaggaaa aagttcagca ttcccagccc gatgtacggt agctgtttgc caagtaaaaa
    72481 ttcgctgcgg gtggtggggg taacgtaaag gttgataatc gaccccaact ctttttcccg
    72541 cactacgcta agggcgctta acatcgacgg aatcatcatc agcagaagcg ggatcaccgc
    72601 cggaacaatc gctggcaggc tttttacgtc cgggttatag cgatagcgtg tctcaatatt
    72661 catcagcccg ctttggctcg cgggcgtgga ttgccgactc gccacatcct gtagccagct
    72721 ctggtgcatg gcctgcacgt aaccttttac cgtttcggcg cgactcggca tcgcaccgtc
    72781 gatccagaca ccgagttcca cgggcgtacc acgcgcgata tcgcgcccga aattgggagg
    72841 gatctcaatc gccacggtga tatcgcccgc acgcatccgg cgatcaagct cgtcataact
    72901 ggtgagcggc ggctgctcga taaagtaacg ggaaccggag agattgagcg tccacgcctg
    72961 gctactgacg gtctggtcgc ggtcgagcac cgcaaagcgc aggttttcca catccatact
    73021 gatgccgtaa cccattatca gcatcaggat caccgttccc atcagcgcca gcgtcgaacg
    73081 taccggatcg cggcgcagtt ccagtgcttc gcgacggctg tagctaaaca gacggcgcag
    73141 gctaaatccc tgacgcggcg cgtgggtggt gtcgtgtatc acgggcggcg cttcggcttc
    73201 gttgctctgc cctgccgctt cctgcaaata ggcgataaat gcctcttcca gactggcggc
    73261 tccgcgtttc tcaaccagtt cctgcggtgt accgctggcg agcacttttc cggcgtgcat
    73321 cagtgagata cggtcgcaac gttccgcttc gttcataaag tgggtggaga taaaaatggt
    73381 cactttgtcc tggcgcgaga gatcgaccat caactgccag aacatatccc tcgccaccgg
    73441 atcgacacca gaagtaggct catcgaggat taacatctcc gggcgatgaa tcaccgccac
    73501 cgccagcgaa agccgctggc gaatgccgag cggcaatgac tccggcagaa catcttcaac
    73561 gtcgttgagc ttaaaacgct cgctcatttc agccactctt gcgggaattt ccgcttccgg
    73621 gatgtgaaac aaacgggcat gtaactcaag gttttgccgc acggtgagtt cgttatagag
    73681 cgaaaacgcc tgcgacatat agcctacacg acggcgggta tcgatatctt ttggatcaac
    73741 cggctgcccg aacagccacg cctcaccttc gctggcgggc agcaatccgg taagcatttt
    73801 catggtggtg gatttaccgc agccgttcga accaagaaaa ccaaaaatct ccccgcgtgg
    73861 aatgcggaaa ttgacgtgat caacggcaac gaaggaacca aaacgcatgg tcagatcgcg
    73921 cgcttcgatg gcaatctctg cgttttccgg ttgatacggt gggatcacta ccgcctgatg
    73981 cgcctggcgt tgcgcttgcg gtaacagatt tataaatgct tcttccagcg tggcgctttg
    74041 cgtttgctgc cgcagctctt cggcgctgcc ggttgccagc acttctccgg cattcatcgc
    74101 taccagccag tcgaagcgtt cggcctcttc catgtaggcg gtggcgacca gcacgctcat
    74161 attgctctgc cgctggcgaa tactgtcgat cagatcccag aactgggcac gggagagcgg
    74221 gtcaaccccc gttgttggct catcaagaat caacagttcc gggtcgtgaa ttaacgcaca
    74281 gcacagccca agtttttgct tcatcccgcc ggagagttta cctgccggac gatcgcgaaa
    74341 cggtgctaac ccggtgctgg tcagcagatc attgattcgg acttcccgct ccgctttgtc
    74401 gtgaccgaac aggcgggcga aaaaatcgac gttttcatac accgacaagg tgtggtagag
    74461 gtttttgccc agcccttgcg gcatccaggc gatgcgcggg caaacgtcac ggcgatgctt
    74521 cgggtcgcgc atatcgccgc ccagcaccat cacattgccc tgctcaatga cgcgggcacc
    74581 ggaaatcaac gacaacaagc tcgacttccc gacgccatcc ggaccaatca gcccgaccat
    74641 acagcgggcc ggaatatcga gagtgatatt gttcagcgca acggtttttc cataatgctg
    74701 gctcacgccc gccagttgcg cgacaggcgg gacgggaacc agttccagat gcgtcattgc
    74761 ggcaacctca ccaccaggtc gtcaggccac ggaagttctt cattcacccg cacccacgct
    74821 acgcccggca aaccggtttt gacatattcc agatgctgct ggagtaattc cggtgggata
    74881 cgcgctttga cgcggaacat tagtttcagc cgttcatcgc tggtttcgac ggtttttggc
    74941 gtgaactggg cgacgctggc gacaaaactg atggttgcag gaatacgcag atctggcgcg
    75001 gcatcgagga tcagccgggc ttcaccgccc agtttcagcg tgcccgcctg ttcggttggc
    75061 aggaagaagg tcatatagac gtcgctgaga tcgaccatat tcagtacccg accacctgcc
    75121 gccagcactt cgcctggctc ggcaacccga tactgcacgc gtccgtcacg cggggctttc
    75181 agttcgctgt catcgatatc tgcggcaatg cgccgttcag tggcttgcgc ggcttcgacg
    75241 cgagtttgcg cctgaatgat attggtgcgt gccgcctcta tagcggcttt agaagcagaa
    75301 acctgggctt ttgctgattc cagcgcagct cgggcgctct cagcggcggc gcggtcgtca
    75361 tccagctgtt gcgcagaaat agctccacgt tgggcaagcg agcgggaacg cgtatgacgt
    75421 ttagccacgg agtccagttc ggcctggcgt tgattgacca gcgactgtgc ggcacgagtt
    75481 tcgctttgcc gttgctccag caaagcctgc gcggcggcaa tggcgctttg tgcctctttg
    75541 atttgcgcga tggcttccag tcgctgttcc tgcaacacgc gagtatccat cttcgccagt
    75601 acttcccctt cgcgaacaaa ctggccttct ttcaccagaa tggtgtcgat acgcccggca
    75661 attttgctgg cgatatccac ttccgtcgct tcaatgcgcc cattgctgac ggcaaagcct
    75721 tccggcacac ctgccgggcg caacagccac cacgccacaa tagcggccac cgccagtaac
    75781 ccgacaaccc accacgccag atggcgctta ctcttatcca taatcgaccc gccataatcc
    75841 ctgtaagcaa acgacgcgtg ttacaactgc cgccgaacga cgttcagcgg attcttccat
    75901 ccgggataag aaggaaacgg caaatacgac cagcgcgcag agcattgagc gccaatgggg
    75961 aaggcttagt gaaaacgggt agtaatgttg tcatcatcag cattcctggc cgtaaatgaa
    76021 acacgcatac tcataagtgt gatctataaa actggctgac aaaacggtat ccatcctgtt
    76081 ggatagcgtt tcacgcctgt tcagttgcag gaacagagca ttccttttta cgttttatca
    76141 acagtctcaa ccagatagta acattaatat tcatgttgtc tatggttcag agctgtaaca
    76201 ttgtgcgcct tctaatcaaa ggcataagtc catttttgtt tttttcatgc tggtaagact
    76261 tccagggagt ggttatctca ggccccgcac cagggttaat ttggcaatta tgcatgctga
    76321 acattcttcc cgcatgttta ctgtcacggg cgttaagacc taaaactgtc cccgtttcac
    76381 ctatgcgttc tctcccacca tttccgttat gtgcaacaac ttgatttata cctaacattt
    76441 gtgagcatgc taatagtgct tcaagtttag gtctgtaatc tgtcgaaaca gaaaaataac
    76501 gattaaagga tcgacaagag atagcccggc tagttttaaa ctttttacca ttaattataa
    76561 tatgatgctt tttattaata attgccgcta attcgaccgg atcgagagca ttggtcattt
    76621 catcaataga gactgttatc ggtcctataa tgtaacgttt tcgctgatca tcaaaagtta
    76681 tcccatgatg atttgccatt attttgtatt tagggtcata gaaacataca ggataatctt
    76741 ttatcccagc gtaggtatcc tcatcaagtg gtttcatacg cgtgaagttc tgcatatagt
    76801 taccattgca attcgtttca tgattaccag ctaatacatg aaaattttca ttgatactca
    76861 ttatgcgttt taacaaatcg atgataaatt gatcgccaca gatattacta aatcgatcgc
    76921 cggtttggtc acccagaaac gtacatggcg tcactgcgcc tttattaata actaaatatg
    76981 gctgaagact ttctaaaaca acatctcttc tgttcagaat actgattatt gtattaacat
    77041 tagtgtcaag ttgctcatta aagttctccc ttaacaatac gttgagaact tcatcttcta
    77101 tattaagcaa ctggctaagg aaattttctc cttcagccat gattcccaaa tgtcccgttg
    77161 cgaaaagcgc gtacaaaact gcaccaactg agccatcggt atcgccaaaa taggtcggcc
    77221 tggtacttgt gggatcaacg ggtgaacaat tggagaaata gattctgtca gcgaactggg
    77281 gttttattat tgtttcgaat ttaatatcaa atacattgta ttgcgatatt ttccagtccc
    77341 agatgggttt gtacgactca gtaactacaa catcattagc attatcattt actgtcatat
    77401 tattaagtaa ataaacaatg ctgcaccaat caatatcggt atcatcaaga gggcaaccgt
    77461 aatttttact attaattgtc acaataaact gataactatt attcctacgt agaatatggg
    77521 cacgcatgcc cgatagcata ctatattttt ctgaatgatt cgtttctatg gtaaatgaaa
    77581 ttttgtcatc ttgaaaagat tgttggaaac gttctatttg ccaatcatca attttttcca
    77641 caacttcacc gagtattgtc ctggcaatac ttgatatatc atcacctata ccatttgcaa
    77701 tatacccaaa cagttcttta gtgaaattag taacatgctc ttgcaccata tcatttgttg
    77761 cgatagtcgt tatactgtcg ttagtacctg ccacagttcc aattttcatc ttttacctca
    77821 tataattcac cgcgaggaat acatagttat catttaatct tatgacaaac acttcatgtt
    77881 cgatggaaaa ataattatat tttgcacaaa tcattcagcc ttaaagcata atatgaaatt
    77941 tcttatctca tagtagagaa agtccctaaa gattgtagag gcgtcatcag tacatgcctc
    78001 tacaatcgat taagaaaaac tatgaaatac tggcaggatt aaaaagacat accacagcac
    78061 tcatggagtt ttaatgtgct tgaaaacaac taagccctgg gacacctttt ttatcaccac
    78121 gttcgttagg tgctatcttc caggaattta gccccatggt ataattatca attttttgat
    78181 gctgatctac ccctaatcca taatggccat atttctgttt actttttttg agcgtctgtc
    78241 cacatttgaa gatatcttca ggttttggtc tgaaagcagt agtgttatta aaataattgt
    78301 catatacagg tgtggatgct atagcattgc ttctaattag atggataaga cgatcatcca
    78361 tgtgttgttt atgtttttta ttaaatatat ttgctaattc ctcaggatca attgggtctt
    78421 tcatctgatt aaatggaacc tgcaaagcac ctagacaata acattcattt tcttcatttc
    78481 ttattattcc gtgatgactg gttaaaactt tcgtttctga gtcataatta caaacatcga
    78541 gcgttttaat taagttatat gtatcaccgg aagagagttt atgatttgct agtcgtgcac
    78601 tatagttacc attaaaattg atttcatgat ttccagccag aacgacgaca tttttattta
    78661 tacgactatc tttattaccc tccatatttc gcatagaatt tagcaaagta aggatatatt
    78721 tatcaccaaa aatagtacta aatctatcgc ctgtatgatc cccaaggaag atacaagggg
    78781 taatcgcatc gccttttaaa aaaacatgtt gatgcaactc attcaagatc atgtctctcg
    78841 tatctaataa agtaaagagt tttttcaatt ttttattttc atatgcgaat gaagccatgt
    78901 cttcgtgttt taataattca cataataaac tccacccctt ttccctgatg cctatatgcc
    78961 ctgaaacgag caatgcataa agtacagcgc ccactcttcc atctgtatcg ccaaaataag
    79021 ccgtgttggg attttgtggg ttaattacac cacactgttt ataaaagtct atttttgaaa
    79081 aaacagattc aacatagcta tcaaactttt ccttagtaaa cgtctcttta gagacagccc
    79141 aacgccagtc aggtttgaaa ttaccactaa gaagagtaaa gaaatttctc tctatgatag
    79201 aaaacccatc aactttataa attatgtctg atttatcccc ggagaaatta ataaaataat
    79261 aaccgtcctt acgctcaatc gtaacggtaa tgggagacga taattttatc acatggttat
    79321 tgtctggtat aattgtaaag ataaacgtgt cagtagtatt atcggcgaaa ctattatatt
    79381 ctttaatatc tatagatggc tcactattgt aaaaccatat tttttctatc tgagaactat
    79441 gttcagttac gttccagcag ccatgttgca ggaaatagat gatatgcagg ccaaaatcgt
    79501 tatctatttg caaatgttta tccagcttat caatttcaac tatctttgaa cgatgttttt
    79561 tatcaacctt gccatggtgt tgatgactgt taactttatc tgaatgctta tcttgatggt
    79621 ttgaagagat gtgcttaaat atattgtgaa atactttcat aaataacccg tgatattttt
    79681 gacttgttag taacaaaata aatgacacat gtctgaaaga catatttacg atgatacaaa
    79741 gcaataatac ttatatgttc gatgatttta agcaccgata ccatccggtc ttttaccaaa
    79801 taatgaaaaa gaatccctcc ctggaagata attgtaaaaa acaacagaat aagaattaat
    79861 cttaggataa atatttattt accatggcct tttgaacgct gaaaagtagc gctctgcaaa
    79921 gtaaatcccg ctatagttat gctatgcatt tgtctttaat gatatctcgt cattatatta
    79981 acaggctgaa attatcatta atgcacattt caatattagc agggatacct gaggagttta
    80041 aagtgaacat atatatcggg tggcttttca aattaatccc tttaattatg ggcttaattt
    80101 gtatcgcgtt aggtggcttt gtgctggaaa gttctgggca aagtgagtat ttcgtcgcgg
    80161 gtcatgtgct gatttctctg gcagccatat gcctggcatt attcactacc gcatttatta
    80221 tcatttcgca gctcacgcgc ggcgttaata cgttttacaa tacattgttc cccattattg
    80281 gctatgcggg gtcaattatc accatgatat ggggttgggc actgttagca ggcaatgatg
    80341 tgatggcaga cgagtttgtt gccggccatg ttatttttgg cgttggtatg attgccgcct
    80401 gtgtatcgac agtggcagcg tcatccggtc actttctgct cattcccaaa aacgcagcag
    80461 ggagcaagag cgatggaaca ccggtacagg cttattcgtc attaatcggt aattgcctca
    80521 ttgccgttcc cgttttactc accctgctcg gtttcatttg gtctattacg ctgttacgta
    80581 gtgccgacat aactccgcat tatgtcgccg gtcacgtatt gcttgggtta accgcaatct
    80641 gtgcctgtct aattggcctt gttgccacaa tcgtccatca aacacgtaat acgttctcat
    80701 ccaaagaaca ctggctgtgg tgttattggg ttattattct cggctcaatc acggtgctgc
    80761 agggcatata cgtgttagtc agctccgatg caagcgtccg actggctccc ggcattattc
    80821 tcatttgcct cggaatgatc tgttacagca tattctcaaa agtctggcta ctggcactgg
    80881 tctggagacg tacctgttcg ttagccaaca gaataccgat gattcccgtc ttcacctgcc
    80941 tgttttgcct tttcctggca tcgtttcttg cggaaatggc gcagaccgac atagggtatt
    81001 ttattccttc gcgggttctg gtcggtttgg gagcggtatg ctttacgttg ttctcaatcg
    81061 tttcaatatt agaagcgggt tctgctaaaa aataattgca acgtaccgga taaaaccagc
    81121 gttgattatt tgcgtaacgc tggtttttct gaggtatcat gaaataacgc agcattaatg
    81181 catagtggtt aagtataaat aaacagcaaa gttgctgatt ttttcaacct gttcatattt
    81241 tataaagatc tggccactgg gggagtttta atcacctttc cctccacctt atattaagca
    81301 tggagggttt cagttcacag gctcattaga aaataatcac aagataacca ctatcaaaat
    81361 tagctcattt ttaatgcgac tctaataatt ttcatcttta ggaaatagct caggacgact
    81421 tcgctgcaat caaatcctgc gcacaagccc acgcactcga ccacgcccac tggaaattat
    81481 agccccccag ccaaccggtg acgtccatca cttcgccgat gaagtacagc cctggcactt
    81541 tgcgcgcttc catcgtccgt gaagagagtt cgttggtgtc cacgccgccg agcgtcactt
    81601 cagcagtacg atagccttca gtgccgttgg gttgtacgcg ccagtcggtc aatgtgctaa
    81661 tcagtgcctg ttggtcacgc acgttgagct gttttagcga aacatccggg atttgcccga
    81721 gttgctgtaa acgttcaacc aaccgcttcg gtagatgaac cgccagtgtg tttttcaggc
    81781 tctgattcgg atgtgcatta cgctgctcat tcaggaaggt ttcgaggtcc acatccggta
    81841 gcagattgat actgacaaat tcacccggtt gccagtagct ggaaatttgc aacaccgccg
    81901 gtccagataa gccgcggtgg gtgaagagta agttctcacg gaaaacggtg ccgttttcag
    81961 cggtaatcac ggaaggcacc gccacgcccg ccagcacctg taactcttcg agcaacggtt
    82021 tatgcagagt gaatggcacc aggcccgcgc gggtcggcag cacgttgagg ccaaattgtt
    82081 cggcaatctt ataaccaaac ggcgatgcgc ccagccccgg cattgacagc ccaccggtcg
    82141 cgatgaccag cttttcgcaa ccgacagtca tgccgttcag ttcaagcgtg aagcctgttt
    82201 catccttcgc cacactcagc acttcgctac gcaatctgaa ggtcacattg cccttctcgc
    82261 actcatccac cagcatgtcg acaatctgct gcgcggagtc atcgcagaag agttgcccta
    82321 acgttttctc gtgccaggtg atgccgtgtt tattgaccag atcaatgaaa tcccactggg
    82381 taaaacgcgc gagtgcagac ttacaaaaat gcggattctg gctcagataa gcgcccggtt
    82441 cgacataaag gttggtaaag ttgcagcgcc caccgccaga catgaggatt ttgcgccctg
    82501 gttttttacc attatcgatc agcagaaccc ggcgtcctgc ctgacctgcc agcgcagaac
    82561 agaacatacc tgccgcacca gcgcctataa taatggcatc aaacctttcc acgttgcgct
    82621 cctctaagaa aaaacgggcg tgaattgtaa agattcctca gtggtcgcac cagcatcaat
    82681 attactaaaa ggaagtattt gcctgaatta tataagataa ttattttttg agtgaaatcc
    82741 atacaggagg caaatcaaaa aaagtctata tttcactttg cccgcgccgc gaaagtcact
    82801 gataatgcgc cgcgttcatg tcctcaaaat ggcgtaacgt cctatgctac atttgtttgc
    82861 tggcctggat ttgcataccg ggctgttatt attgcttgca ctggcttttg tgctgttcta
    82921 cgaagccatc aatggtttcc atgacacagc caacgccgtg gcaaccgtta tctatacccg
    82981 cgcgatgcgt tctcagctcg ccgtggttat ggcggcggtg ttcaactttt tgggtgtttt
    83041 gctgggtggt ctgagtgttg cctatgccat tgtgcatatg ctgccgacgg atctgctgct
    83101 taatatggga tcgtctcatg gccttgccat ggtgttctct atgttgctgg cggcgattat
    83161 ctggaacctg ggtacctggt actttggttt acctgcatcc agctctcata cgctgattgg
    83221 cgcgatcatc gggattggtt taaccaatgc gttgatgacc gggacgtcag tggtggatgc
    83281 actcaatatc ccgaaagtat taagtatttt cggttctctg atcgtttccc ctattgtcgg
    83341 cctggtgttt gctggcggtc tgattttctt gctgcgtcgc tactggagcg gcaccaagaa
    83401 acgcgcccgt atccacctga ccccagcgga gcgtgaaaag aaagacggca agaaaaagcc
    83461 gccgttctgg acgcgtattg cgctgatcct ttccgctatc ggcgtggcgt tttcgcacgg
    83521 cgcgaacgat ggtcagaaag gtattggtct ggttatgttg gtattgattg gcgtcgcacc
    83581 agcaggcttc gtggtgaaca tgaatgccac gggctacgaa atcacccgta cccgtgatgc
    83641 catcaacaac gtggaagctt actttgagca gcaccctgcg ctgctgaaac aggctaccag
    83701 tgctgatcag ttagtaccgg ctccggaagc tggcgcaacg caacctgcgg agttccattg
    83761 ccatccgtcg aataccatta acgcgctcaa ccgcctgaaa ggcatgttga ctaccgatgt
    83821 ggaaagctac gataagctgt cgcttgatca acgtagccag atgcgccgca ttatgctgtg
    83881 cgtttctgac actatcgata aagtggtgaa gatgcctggt gtgagtgctg acgatcagcg
    83941 tctgttgaag aaactgaagt ccgacatgct tagcaccatc gagtatgcgc cggtgtggat
    84001 catcatggcg gtcgcgctgg cgttaggtat cggtacgatg attggctggc gtcgtgtggc
    84061 aacgactatc ggtgagaaaa tcggtaagaa aggcatgacc tacgctcagg ggatgtctgc
    84121 ccagatgacg gcggcagtgt ctatcggcct ggcgagttat accgggatgc cggtttccac
    84181 tacccacgta ctctcctctt ctgtcgcggg gacgatggtg gtagatggcg gcggcttaca
    84241 gcgtaaaacc gtaaccagta ttctgatggc ctgggtattt acccttccgg ctgcggtact
    84301 gctttccggc gggctgtact ggctctcctt gcagttcctg taatcgtacg caccaaaacg
    84361 agcgggtcag tttactggcc cgcttttttt atgcgtcatt attgctgaag tgcttaatgc
    84421 caaatcatca atgcaatcag gctgaccacc accagaccac acaatgcgct ggtcagaata
    84481 aactgccgac gcacccgctc acagcggcga ataaactcat catcgtgatg atcgcgataa
    84541 cgttgggcat agatatacca aacgagacgc acctgtttgt tgggttggcc atgtgaggta
    84601 aaaaagcccc ctccatcaac atattgatag agcaatggat cgcagttacg cagtaccact
    84661 aacaacgcgc gtagtgatga gaaatagcgc gccatgttaa caatgcaaac gacacataaa
    84721 gcccaaaata atgcgacggt gcttatcata cctcctcccc ggcgacctgc ccgcggagtt
    84781 ccaccccggg gctaccgctc ccgatacgct gccaatcagt taacaccagg tcctggagaa
    84841 accgcttttg tggtgaccaa catccgagcg gctctataga tagtgtagga gatcaggttg
    84901 ttttttttcc agaaggttaa ccactatcaa tatattcatg tcgaaaattt gtttatctaa
    84961 cgagtaagca aggcggattg acggatcatc cgggtcgcta taaggtaagg atggtcttaa
    85021 cactgaatct ttacggctgg gttagccccg cgcacgtagt tcgcaggacg cgggtgacgt
    85081 aacggcacaa gaaacgctag ctggccagtc atcgacaact ttatggaagg agtaacacta
    85141 tggcttataa acacattctc atcgcggtcg acctctcccc ggaaagcaaa gttctggtag
    85201 agaaagcagt ctctatggct cgcccctaca atgcgaaagt ttctctgatc cacgtagatg
    85261 taaactactc tgacctatac accgggctta ttgatgtgaa tctgggtgat atgcagaaac
    85321 gcatctctga agagacacat catgcgctga ccgagctttc cactaatgca ggctacccaa
    85381 tcactgaaac cctgagcggc agcggcgatc tgggccaggt tctggtcgat gcaatcaaga
    85441 aatacgatat ggatctggtg gtttgtggtc accaccagga cttctggagc aaactgatgt
    85501 cttccgcacg tcagctgatc aacaccgttc acgttgatat gctgattgtt ccgctacgcg
    85561 acgaagaaga ataatcttcc ctctacgacg tgttcctgaa cgctcgcata tgcgggcgtt
    85621 ttgctttttg gcgcgccttg ttaccttgca gcgtaaacac cttatctggc ctacggtctg
    85681 cgtacgcaat caaaatcccc tgccaataca acatttaaca ccatcatact ttccatcatt
    85741 agtgtgatca gctggttatt ttctgttgta atagtgtatt aatctattca cagcatcaat
    85801 attaagaatc tctgacagat gtaaactttt tcgcgcgtta tcccttacgc gttcatactt
    85861 ttcaggatgg tattggaagg ttaataaata tgaatacaac aacacccatg gggatgctgc
    85921 agcaacctcg cccatttttc atgatctttt ttgtcgagtt atgggagcga ttcggctact
    85981 acggcgtgca gggcgtactg gctgttttct tcgttaagca gcttggattc tcgcaagaac
    86041 aggcttttgt cacttttggt gcttttgctg cgctggtcta tggcctcatt tccattggcg
    86101 gctatgtcgg cgaccacctg ctggggacca aacgcaccat tgttctcgga gcacttgtgc
    86161 tggcgattgg ctacttcatg accggcctgt cgctacttaa gcctgacctg attttcatcg
    86221 ccctggggac tatcgctgtc ggtaacggcc tgtttaaagc taacccagcc agcttgcttt
    86281 cgaagtgcta tccgccgaaa gatccgcggc ttgatggcgc atttaccctg ttctatatgt
    86341 cgatcaacat cggctcgttg atagcgttat cgctggcccc tgtgatcgct gatagattcg
    86401 gctattcagt cacctacaac ctgtgcggtg cgggattaat tatcgcgcta ttggtttaca
    86461 tcgcctgtcg cggaatggtg aaagatattg gttctgaacc cgacttctgc ccgatgagct
    86521 tcagtaaact gttgtacgtg ttacttggca gcgtggtgat gatcttcgtc tgtgcatggc
    86581 tgatgcacaa cgtagaagtc gccaatctgg tgctgattgt tctctccatc gtcgtcacca
    86641 tcattttctt tcgtcaggca ttcaagctgg ataaaactgg gcgcaataaa atgtttgtcg
    86701 cctttgtcct gatgctcgaa gcggtggtgt tttacattct ctacgcccag atgccaacgt
    86761 cgctgaactt ctttgccatc aacaacgtgc atcatgaaat tctcggcttt tccatcaacc
    86821 cggtcagctt ccaggcgctt aacccgttct gggtggtact cgccagccca atactggcag
    86881 gcatttacac gcatctgggt aacaaaggca aagacctctc gatgccgatg aaatttactc
    86941 tcggaatgtt tatgtgctcg ctgggctttt tgacggctgc agcggctgga atgtggtttg
    87001 cggatgccca ggggctgaca tcgccatggt ttatcgtgct ggtgtactta ttccagagcc
    87061 tgggtgaact gtttattagc gccctgggcc tggcgatgat tgctgccctg gtgccgcagc
    87121 atttgatggg ctttattctc gggatgtggt tcctgacgca ggctgccgcg ttcttgctgg
    87181 gcggctatgt ggcaacattt accgcagtac cagacaacat taccgatccg cttgagacgt
    87241 tgcccgtcta taccaacgtg tttggtaaga ttggcctggt tacgctgggc gttgcggtgg
    87301 tgatgctgct gatggtgccg tggctgaaac gcatgattgc gacacccgaa agccattaat
    87361 tattcttgca gaaagcaggg tagcgataac gctaccctgc tttactttta caccggcgtt
    87421 ccggcataaa tatcaaagcg atgccctttg gtgactaccg cgtttggcgt ggcgacattc
    87481 gccagcggtg gcgcgtagtc cgggcgcttt accaccacgc gtttggtcgc caacaagcgt
    87541 gcaggctcca gtaatccatc ggcatcaaga tccggtccca ccagcgactg aaagacgcgc
    87601 atctcttttt tcaccagcgc gcttttctgc ttatgcggga acatcggatc gagatagacc
    87661 acctgcgggc gcggagtaat atcagtcagc gccgtcaggc tggaggcgtg aattaactgc
    87721 aaacgctcct gcaaccagcc gccgatttcc gcatccgcat aaccacgcgc caggccgtcg
    87781 tcgagcaatg cagcaacgac tggattacgc tccagcatcc gcacgcggca gccgactgaa
    87841 gccagcacaa aggcatcgcg cccaagtcct gcagtggcat ccaccacatc cggcaaataa
    87901 tcgcctttaa tgcccaccgc tttcgccact gcctccccac gtccgccgcc gaatttgcgt
    87961 cggtgcgcca tcgctccgcc aacaaaatca acaaagatgc cgccaagttt cggctcatca
    88021 cgcttgcgca attccagatg ttccggcgtt aacaccagcg ccatcaggtt gtcttcatcg
    88081 tgctccagcc cccagcgggc cgccagaaca gataaggcac cgtctccggt gcctgtttca
    88141 tcaattaagc agattttcac tgaatgatca gcccttaatg ccgtaatgct ccagcatcgc
    88201 atccagctgc ggttcacgac cacggaagcg tttgaacaga tccatcggct cttctgaacc
    88261 gccacggctc agaatgttgt cgaggaacga ctgtccggtt tcacggttga aaatgccctc
    88321 ttcctcaaag cgcgagaagg catctgccgc cagcacgtcg gcccacaggt agctgtagta
    88381 acctgcggca taaccaccgg cgaaaatatg gctgaaagcg tgcgggaaac ggccccagga
    88441 tggagacggt actacggcaa ccagtttctt gatttctgcc agagtttcga ggattttcgc
    88501 tccctgatcc gggcggaact cggcatggag gcggaaatcg aacagaccga actccagctg
    88561 gcgcagaata aacagcgccg cctggtagtt cttcgccgcc agcattttat ccagcaactc
    88621 tttcggcagc ggttcgccgg tttcatagtg accggagata aacgccagcg cctccggctc
    88681 ccagcaccag ttttccataa actggctcgg cagttcgacc gcatcccacg gcaccccgct
    88741 gataccagac actccagcgg tttcaatgcg ggtcagcata tgatgcaggc cgtgaccgaa
    88801 ctcgtggaac agggtgatca cttcgtcatg ggtaaacagc gccggtttac catttaccgg
    88861 gcggttgaag ttgcaggtca gatacgcgac cggtttttgc agcgaaccgt cggctttacg
    88921 catctggcct acgcagtcat ccatccacgc cccgccgcgt ttgttttcac gggcatacag
    88981 gtcgaggtag aagctgccgc gcagttcgtt gttctcgtca tacagttcga agaaacgtac
    89041 atccggatgc cagacatcaa catctttacg ctctttagcg gtgatgccgt aaatacgttt
    89101 caccacttca aacaggccgt taaccgcttt gttttccggg aagtacggac gcagttgttc
    89161 atcgctgatg ctgtagaggt gctgtttctg tttttcgctg tagtaagcga tatcccacgg
    89221 ctgcaactca tcgacgccaa attcggcttt ggcgaaggca cgcagttgcg ccagctcttt
    89281 ttcgccttgt ggacgcgcgc gttttgccag atcggttaag aaatccagca cctgctgcgg
    89341 gttttctgcc attttagtag caagggattt aaaggcatag ttttcaaagc ccagcagttg
    89401 cgccagttcg tgacgcagcg cgaggatctc ttccatcacc ttgctgttgt cccacttacc
    89461 ggcgttcggg ccttgatcgg aagcgcgggt gctgtaagca cgatacatct cttcacgcag
    89521 agcctggttg tcgcagtagg tcattaccgg caagtagctt gggatatcca gcgtcagcaa
    89581 ataaccttcc agctctttcg cttcggcctg ggcttttgcc gcagccagcg cgctttctgg
    89641 catccccgcc agctccgctt cgtcggtaac gagtttggtc cagcccatcg tcgcatcgag
    89701 gacgttgttg ctgtactggt tgcccagttc agaaagacgg gtagcaattt cgccgtaacg
    89761 ctgctgtttc tctttcggca gaccgatgcc agagagttcg aagtcgcgca gtgcgttatc
    89821 aaccgctttt ttctgcgccg tgttcagcgt ggcgtaatga tcgccatcgc gcaggtcgcg
    89881 atatgcctta tacagccctt catgttgccc tacccaggtg ctgtattctg acagcagcgg
    89941 cagggtttgt tcgtacgctt cacgcagttc cgggctattt ttcaccgagt tcaggtggct
    90001 gaccggggag aagatacgcc ccagcacatc gtccacttcc gccaacggct ggcagagatt
    90061 ttcccaggtg tacggtgccc cttgcgctac tacgcgctcc acattttcgc ggcagtcgtt
    90121 cagcgcctta gtcacggctg gaacgacatg ttccgggaga attttagaaa acggaggcaa
    90181 ttcaaaggga gtcagtaacg gattcgtcat tagcgcagtc ctggttaaag aggttaagga
    90241 agcgctcaac aggcgcttta cataatgtgt gtagcatggg gttaagtgta gtgaatttca
    90301 atgagaaacg ttacgctttc gcggcggcgg cctcttttcg gtatactgtc ctgataggct
    90361 tttgtgcgcc ccgaatacgg gccgattttt acttaccgga acacctttac ccatgctcag
    90421 ttatcgccac agctttcacg ctggcaacca cgccgacgtc cttaaacata ccgttcagag
    90481 cctgatcatc gagtcgctga aagagaaaga taaaccgttt ctctatctcg acacccacgc
    90541 aggggccggg cgttatcagt taggcagcga acatgccgag cgtaccggcg aatatctcga
    90601 aggtatcgcc cgtatctggc agcaggacga tttgcctgcg gagctggagg cgtacatcaa
    90661 tgtggtaaaa cacttcaacc gtagcggtca gttgcgttat taccccggtt cgccgttgat
    90721 tgctcgccag ctactgcgtg aacaggacag cctgcaactg accgaactgc acccgagcga
    90781 ttacccgttg ctgcgttctg aatttcagaa agacagccgt gcgcgcgtcg aaaaagccga
    90841 tggtttccag cagcttaagg ccaaactgcc gccggtttcc cgccgtggtt taatccttat
    90901 cgacccgccg tatgaaatga aaaccgacta tcaggcggtg gtcagcggga tagcagaagg
    90961 ttacaaacgt ttcgccactg gcacttacgc attgtggtat ccagtggtgc tgcgccagca
    91021 aattaagcgc atgatccacg acctggaagc gaccggcatt cgcaaaattc tgcaaattga
    91081 actggcggtg ctgccagaca gcgatcgccg tggcatgacc gcttccggca tgattgtgat
    91141 taacccgccg tggaagctgg aacagcagat gaataacgtg ctgccgtggc tgcacagcaa
    91201 actggttcca gcaggcaccg ggcacgccac cgtaagctgg atcgtgccag agtaattgca
    91261 gccattgctg gcacctatta cgtctcgcgc tacaatcgcg gtaatcaacg ataaggacaa
    91321 tatgtcatga ctaaacacta tgattacatc gccatcggcg gcggcagcgg cggtatcgcc
    91381 tccatcaacc gcgcggctat gtacggccag aaatgcgcac tgattgaagc caaagagctg
    91441 ggcggcacct gcgtaaatgt tggctgtgtg ccgaaaaaag tgatgtggca cgcggcgcaa
    91501 atccgtgaag cgatccacat gtacggcccg gattatggtt tcgataccac catcaataag
    91561 ttcaactggg aaacgttgat cgccagccgt accgcctata tcgaccgtat tcatacttcc
    91621 tatgagaacg tgctcggtaa aaataacgtt gatgtaatca aaggctttgc ccgcttcgtt
    91681 gatgccaaaa ccattgaagt caacggcgaa accatcacgg ccgatcatat tctgatcgcc
    91741 accggtggtc gtccgagcca cccggatatt ccaggcgtgg aatacggtat tgattccgat
    91801 ggtttcttcg cccttcctgc tttgccagag cgcgtggcgg ttgttggcgc aggttacatc
    91861 gccgtggaac tggcgggcgt gattaacggc ctcggcgcga aaacgcatct gtttgtgcgt
    91921 aaacatgcgc cgctgcgcag cttcgacccg atgatctctg aaacgctggt cgaagtgatg
    91981 aacgccgaag gcccgcagtt gcacaccaac gccatcccga aagcagtagt gaaaaatgct
    92041 gatggtagcc tgacgctgga gctggaagat gggcgcagtg aaacggtgga ttgcctgatt
    92101 tgggcgattg gtcgcgagcc tgccaatgac aacatcaacc tggaagccgc tggtgtaaaa
    92161 accaacgaaa aaggctatat cgtcgtcgat aaatatcaaa ataccaacgt tgaaggtatt
    92221 tacgcggtgg gcgataacac gggtgcagtg gaactgacac cggtggcagt tgctgcgggt
    92281 cgccgtctct ctgaacgcct gtttaataac aagccggatg agcatctgga ttacagcaac
    92341 attccgaccg tggtcttcag ccatccgccg attgggactg ttggtttaac ggaaccgcag
    92401 gcacgcgagc agtacggcga cgatcaggtg aaagtgtata aatcctcttt caccgcgatg
    92461 tataccgccg tcaccactca ccgccagccg tgccgcatga aactggtatg cgttggaccg
    92521 gaagagaaga ttgtcggaat tcacggcatt ggttttggta tggacgaaat gttgcagggc
    92581 ttcgcggtgg cactgaagat gggcgcaacc aaaaaagact tcgataatac cgtcgccatt
    92641 cacccgacgg cggcagaaga gttcgtgacc atgcgttaaa tgttaaaggg ctaagagtag
    92701 tgtgctctta gcccttagct acgtttccgc taccagttca gaagctgaag cagaaagcgg
    92761 atcagttcca gcagcgcaat taacgcccca agaacgatga ttgctttatc aatcacccgt
    92821 gttctccata cgatggagtg agaatgcatc cgcttactca tccattgcct gtcacggcgc
    92881 atgccctatt gttagataat ggcattctca ctccggccag agcatcagtt aacggcacca
    92941 cccgtaccac tgatcaggac tttgaaagcg tttatgcgca ttgccagagt gaaaatgcct
    93001 cagagctaac tggataatca tacagtatat tcaggttata aaaccagcat gtccttgcaa
    93061 tagtttcagt atggtattag cattgatgcg ttagatgatg gcattctcac tccagccaga
    93121 gccaccaact cagggctgga aagtaaaaaa ccgacgcaaa gtcggttttt ttacatccgg
    93181 attcggacaa ggcttggtac ggcagcgacc cgatgaaagt atatcaatcc tctttcacca
    93241 ccatgtatag caaactgtcc ctccgccagc tgaagaaatc gctaattctt gcaatgttag
    93301 tcactggcta atagtattga accgttagat aagagctctc tcactccagc cagagccacc
    93361 aactcaggct gaagagtaaa aaaccgacgc aaagtcggtt ttttatgtcc tgattcagac
    93421 ctcctttcaa atgaataccc aactcaaaat tcacacctat taccttcctc tgcacatacg
    93481 aattcgttaa accatatatg tttttgactt atccgcttcg aagagagaca ctaagtgcaa
    93541 caatcaggag cgcaatatgt catttctgtt acccatccaa ctcttcaaaa ttcttgccga
    93601 tgaaacccgt ttgggcatcg ttttactgct cagcgagctg ggagagttat gcgtctgcga
    93661 tctctgcact gccctcgatc agtcgcagcc caagatctcc cgccatctgg cattactgcg
    93721 tgaaagtggg ctattgctgg accgcaagca aggaaagtgg gttcattacc gtttatcgcc
    93781 gcatattcca gcatgggcgg cgaaaattat tgagcaggcc tggcgatgcg aacaggaaaa
    93841 ggttcaggtg attgtccgta acctcgccag acaaaattgt tccgcagaca gtaagaatat
    93901 ttgtagttaa aaaatttatc taaacacata tgaatattcg aatgtgtttt atcaaggagg
    93961 catgatgtta ctggcgggcg ctatctttgt cctgaccatc gtattggtta tctggcagcc
    94021 gaaaggttta ggcatcggct ggagtgcgac actgggcgcc gtactggcgt tagttactgg
    94081 cgtggtccat ccgggcgata ttccggtggt gtggaatatc gtctggaacg cgacggcggc
    94141 gtttatagcc gtcattatca tcagcttgct gctggatgag tccggctttt ttgaatgggc
    94201 ggcgctgcac gtctcacgct ggggcaatgg gcgcggtcgc ttgctgttta cctggattgt
    94261 cctgctcggt gctgccgttg cagctctgtt tgccaatgat ggcgcggcgc ttattttgac
    94321 accgattgtc atcgccatgc tgctggcttt agggttcaac aaaggcacta cgctggcgtt
    94381 tgttatggcg gccggattca ttgccgatac cgccagcctg ccgcttattg tctctaacct
    94441 ggtgaatatc gtttcggcag atttctttag cctcgacttt cgcgaatacg cctcggtgat
    94501 ggtgccggtg gatatcgctg caatactggc taccctggtg atgttgcatc tctatttccg
    94561 caaagatatt ccgcagaact acgacatggc gctgctaaaa tctcccgcag aagcggttaa
    94621 agatcctgcc accttcaaaa ctggcggggt tgttttactg cttctgctgg tgggattttt
    94681 cgtcctggaa ccgctcggca ttccggtaag cgccattgcg gcagtgggcg cgctgatatt
    94741 atttgtcgtc gctaaacgcg gtcatgcgat taatacgggt aaggtgctgc gcggtgcgcc
    94801 ctggcagatt gtcatcttct cgctcggcat gtatctggtg gtttatggcc tgcgcaatgc
    94861 cggattaacg gaatatcttt ctgg
//
LOCUS       DAMZFT010000005        83041 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.005, whole genome shotgun sequence.
ACCESSION   DAMZFT010000005 DAMZFT010000000
VERSION     DAMZFT010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 83041)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 83041)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..83041
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.005"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(<1..134)
                     /gene="tuf"
                     /locus_tag="PN977_000389"
     CDS             complement(<1..134)
                     /gene="tuf"
                     /locus_tag="PN977_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HDI6040470.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     R"
     gene            complement(205..2319)
                     /gene="fusA"
                     /locus_tag="PN977_000390"
     CDS             complement(205..2319)
                     /gene="fusA"
                     /locus_tag="PN977_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020455498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HDI6040471.1"
                     /translation="MARTTPIARYRNIGISAHIDAGKTTTTERILFYTGVNHKIGEVH
                     DGAATMDWMEQEQERGITITSAATTAFWSGMAKQYEPHRINIIDTPGHVDFTIEVERS
                     MRVLDGAVMVYCAVGGVQPQSETVWRQANKYKVPRIAFVNKMDRMGANFLKVVNQIKT
                     RLGANPVPLQLAIGAEEHFTGVVDLVKMKAINWNDADQGVTFEYEDIPADMVELANEW
                     HQNLIESAAEASEELMEKYLGGEELTEAEIKGALRQRVLNNEIILVTCGSAFKNKGVQ
                     AMLDAVIDYLPSPVDVPAINGILDDGKDTPAERHASDDEPFSALAFKIATDPFVGNLT
                     FFRVYSGVVNSGDTVLNSVKAARERFGRIVQMHANKREEIKEVRAGDIAAAIGLKDVT
                     TGDTLCDPDAPIILERMEFPEPVISIAVEPKTKADQEKMGLALGRLAKEDPSFRVWTD
                     EESNQTIIAGMGELHLDIIVDRMKREFNVEANVGKPQVAYRETIRQKVTDVEGKHAKQ
                     SGGRGQYGHVVIDMYPLEPGSNPKGYEFINDIKGGVIPGEYIPAVDKGIQEQLKAGPL
                     AGYPVVDMGIRLHFGSYHDVDSSELAFKLAASIAFKEGFKKAKPVLLEPIMKVEVETP
                     EENTGDVIGDLSRRRGMLKGQESEVTGVKIHAEVPLSEMFGYATQLRSLTKGRASYTM
                     EFLKYDEAPSNVAQAVIEARGK"
     gene            complement(2416..2886)
                     /gene="rpsG"
                     /locus_tag="PN977_000391"
     CDS             complement(2416..2886)
                     /gene="rpsG"
                     /locus_tag="PN977_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015873274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HDI6040472.1"
                     /translation="MPRRRVIGQRKILPDPKFGSELLAKFVNILMVDGKKSTAESIVY
                     SALETLAQRSGKSELEAFEVALENVRPTVEVKSRRVGGSTYQVPVEVRPVRRNALAMR
                     WIVEAARKRGDKSMALRLANELSDAAENKGTAVKKREDVHRMAEANKAFAHYRW"
     gene            complement(2983..3357)
                     /gene="rpsL"
                     /locus_tag="PN977_000392"
     CDS             complement(2983..3357)
                     /gene="rpsL"
                     /locus_tag="PN977_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005306278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HDI6040473.1"
                     /translation="MATVNQLVRKPRARKVAKSNVPALEACPQKRGVCTRVYTTTPKK
                     PNSALRKVCRVRLTNGFEVTSYIGGEGHNLQEHSVILIRGGRVKDLPGVRYHTVRGAL
                     DCSGVKDRKQARSKYGVKRPKA"
     gene            complement(3483..3770)
                     /gene="tusB"
                     /locus_tag="PN977_000393"
     CDS             complement(3483..3770)
                     /gene="tusB"
                     /locus_tag="PN977_000393"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusB"
                     /protein_id="HDI6040474.1"
                     /translation="MLHTLHRSPWLTDFAALLRLLSEGDELLLLQDGVTAGVDGNCYL
                     ESLRNAPIKVYALNEDLIARGLTGRISNDIIPIDYTDFVRLTVKHSSQMAW"
     gene            complement(3778..4137)
                     /gene="tusC"
                     /locus_tag="PN977_000394"
     CDS             complement(3778..4137)
                     /gene="tusC"
                     /locus_tag="PN977_000394"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusC"
                     /protein_id="HDI6040475.1"
                     /translation="MKRIAFVFSTAPHGTAAGREGLDALLATSALTDDLAVFFIADGV
                     FQLLPGQKPDAVLARDYIATFKLLGLYDIEQCWVCAASLRERGLDPQTPFVVEATPLE
                     ADALRRELANYDVILRF"
     gene            complement(4137..4523)
                     /gene="tusD"
                     /locus_tag="PN977_000395"
     CDS             complement(4137..4523)
                     /gene="tusD"
                     /locus_tag="PN977_000395"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusD"
                     /protein_id="HDI6040476.1"
                     /translation="MRFAIVVTGPAYGTQQASSAFQFAQALIAEGHELSCVFFYREGV
                     YNANQLTSPASDEFDLVRGWQQLNAQHGVALNICVAAALRRGIVDETEAGRLGLASSN
                     LQSGFTLSGLGALAEASLTCDRVVQF"
     gene            complement(4523..5245)
                     /locus_tag="PN977_000396"
     CDS             complement(4523..5245)
                     /locus_tag="PN977_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDI6040477.1"
                     /translation="MSRSLLTNETSELDLLDQRPFDQTDFDILKSYEAVVDGLAMLIG
                     SHCEIVLHSLQDLKCSAIRIANGEHTGRKIGSPITDLALRMLHDMTGADSSVSKCYFT
                     RAKSGVLMKSLTIAIRNREQRVIGLLCINMNLDVPFSQIMSTFVPPETPDVGSSVNFA
                     SSVEDLVTQTLEFTIEEVNADRNVSNNAKNRQIVLNLYEKGIFDIKDAINQVADRLNI
                     SKHTVYLYIRQFKSGDFQGQDK"
     gene            complement(5412..6224)
                     /gene="fkpA"
                     /locus_tag="PN977_000397"
     CDS             complement(5412..6224)
                     /gene="fkpA"
                     /locus_tag="PN977_000397"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HDI6040478.1"
                     /translation="MKSLFKVTLLATTMAVALHAPITFAAEAAKPATTADSKAAFKND
                     DQKSAYALGASLGRYMENSLKEQEKLGIKLDKDQLIAGVQDAFADKSKLSDQEIEQTL
                     QAFEARVKSSAQAKMEKDAADNEAKGKEYREKFAKEKGVKTSSTGLVYQVVEAGKGEA
                     PKDSDTVVVNYKGTLIDGKEFDNSYTRGEPLSFRLDGVIPGWTEGLKNIKKGGKIKLV
                     IPPELAYGKAGVPGIPPNSTLVFDVELLDVKPAPKADAKPEADAKAADSAKK"
     gene            6445..6663
                     /locus_tag="PN977_000398"
     CDS             6445..6663
                     /locus_tag="PN977_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein SlyX"
                     /protein_id="HDI6040479.1"
                     /translation="MQDLSLEARLAELESRLAFQEITIEELNVTVTAHEMEMAKLRDH
                     LRLLTEKLKASQPSNIASQAEETPPPHY"
     gene            complement(6713..7303)
                     /gene="slyD"
                     /locus_tag="PN977_000399"
     CDS             complement(6713..7303)
                     /gene="slyD"
                     /locus_tag="PN977_000399"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HDI6040480.1"
                     /translation="MKVAKDLVVSLAYQVRTEDGVLVDESPVSAPLDYLHGHGSLISG
                     LETALEGHEVGDKFDVAVGANDAYGQYDENLVQRVPKDVFMGVDELQVGMRFLAETDQ
                     GPVPVEITAVEDDHVVVDGNHMLAGQNLKFNVEVVAIREATEEELAHGHVHGAHDHHH
                     DHDHDGCCGGHGHDHGHEHGGEGCCGGKGNGGCGCH"
     gene            complement(7398..7598)
                     /locus_tag="PN977_000400"
     CDS             complement(7398..7598)
                     /locus_tag="PN977_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheV family putative metal-binding protein"
                     /protein_id="HDI6040481.1"
                     /translation="MAIRKRFIAGAKCPACQAQDSMAMWRENNIDIVECVKCGHQMRE
                     ADKEARDHVRKDEQVIGIFHPD"
     gene            complement(7608..9413)
                     /gene="kefB"
                     /locus_tag="PN977_000401"
     CDS             complement(7608..9413)
                     /gene="kefB"
                     /locus_tag="PN977_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefB"
                     /protein_id="HDI6040482.1"
                     /translation="MEGSDFLLAGVLFLFAAVAAVPLASRLGIGAVLGYLLAGIAIGP
                     WGLGFISDVDEILHFSELGVVFLMFIIGLELNPSKLWQLRRSIFGVGAAQVLLSAALL
                     AGLLMLTHFSWQAAVVGGIGLAMSSTAMALQLMRDKRMNRSESGQLGFSVLLFQDLAV
                     IPALALVPLLAGSADEHFDWMKIGMKVLAFVGMLIGGRYLLRPVFRFIAASGVREVFT
                     AATLLLVLGSALFMDALGLSMALGTFIAGVLLAESEYRHELETAIDPFKGLLLGLFFI
                     SVGMSLNLGVLYTHLLWVVISVVVLVAVKILVLYLLARLYGVRSSERMQFAGVLSQGG
                     EFAFVLFSTASSQRLFQGDQMALLLVTVTLSMMTTPLLMKLVDKWLSRQFNGPEEEDE
                     KPWVNDDKPQVIVVGFGRFGQVIGRLLMANKMRITVLERDISAVNLMRKYGYKVYYGD
                     ATQVDLLRSAGAEAAESIVITCNEPEDTMKLVEICQQHFPHLHILARARGRVEAHELL
                     QAGVTQFSRETFSSALELGRKTLVTLGMHPHQAQRAQLHFRRLDMRMLRELIPMHADT
                     VQISRAREARRELEEIFQREMQQERRQLDGWDEFE"
     gene            complement(9413..9964)
                     /gene="kefG"
                     /locus_tag="PN977_000402"
     CDS             complement(9413..9964)
                     /gene="kefG"
                     /locus_tag="PN977_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312229.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     ancillary protein KefG"
                     /protein_id="HDI6040483.1"
                     /translation="MSQPAKVLLLYAHPESQDSVANRVLLKPATQLSNVTVHDLYAHY
                     PDFFIDIPREQALLREHEVIVFQHPLYTYSCPALLKEWLDRVLSRGFASGPGGNQLAG
                     KYWRSVITTGEPESAYRYDALNRYPMSDVLRPFELAAGMCRMHWLSPIIIYWARRQSA
                     QELASHARAYGDWLANPLSPGGR"
     gene            10095..12008
                     /locus_tag="PN977_000403"
     CDS             10095..12008
                     /locus_tag="PN977_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDI6040484.1"
                     /translation="MIVFSSLQIRRGVRVLLDNATATINPGQKVGLVGKNGCGKSTLL
                     ALLKNEISADGGSYTFPGSWQLAWVNQETPALPQAALEYVIDGDREYRQLEAQLYDAN
                     ERNDGHAIATIHGKLDAIDAWSIRSRAASLLHGLGFSNEQLERPVSDFSGGWRMRLNL
                     AQALICRSDLLLLDEPTNHLDLDAVIWLEKWLKSYQGTLILISHDRDFLDPIVDKIIH
                     IEQQSMFEYTGNYSSFEVQRATRLAQQQAMYESQQERVAHLQSYIDRFRAKATKAKQA
                     QSRIKMLERMELIAPAHVDNPFRFSFRAPESLPNPLLKMEKVSAGYGDRIILDSIKLN
                     LVPGSRIGLLGRNGAGKSTLIKLLAGELAPVSGEIGLAKGIKLGYFAQHQLEYLRADE
                     SPIQHLARLAPQELEQKLRDYLGGFGFQGDKVTEETRRFSGGEKARLVLALIVWQRPN
                     LLLLDEPTNHLDLDMRQALTEALIEFEGALVVVSHDRHLLRSTTDDLYLVHDRKVEPF
                     DGDLEDYQQWLSDVQKQENQTDEAPKENANSAQARKDQKRREAELRAQTQPLRKEIAR
                     LEKEMEKLNAQLAQAEEKLGDSELYDQSRKAELTACLQQQASAKSGLEECEMAWLEAQ
                     EQLEQMLLEGQSN"
     gene            12008..13030
                     /locus_tag="PN977_000404"
     CDS             12008..13030
                     /locus_tag="PN977_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HDI6040485.1"
                     /translation="MAQITTTDANEFSSSAEFTPMRGFSNCHLQTMLPRLFRRKVKFT
                     PYWQRLELPDGDFVDLAWSEDPAQANHKPRLVVFHGLEGSLNSPYAHGLVEAAQKRGW
                     LGVVMHFRGCSGEPNRMHRIYHSGETEDASWFLRWLQREFGHAPTAAVGYSLGGNMLA
                     CLLAKEGNNLPIDAAVIVSAPFMLEACSYHMEKGFSRVYQRYLLNLLKANAARKLAAY
                     PGTLPINLAQLKSVRRIREFDDLITARIHGYADAIDYYRQCSAMPMLNQIAKPTLIIH
                     AKDDPFMDHQVIPKPESLPPQVEYQLTEHGGHVGFIGGTLLHPQMWLESRIPDWLTTY
                     LEAKSC"
     gene            13024..13242
                     /locus_tag="PN977_000405"
     CDS             13024..13242
                     /locus_tag="PN977_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheU family protein"
                     /protein_id="HDI6040486.1"
                     /translation="MLIPWQDLSPETLENLIESFVLREGTDYGEHERTLEQKVADVKR
                     QLQCGEAVLVWSELHETVNIMPRSQFRE"
     gene            13296..14165
                     /locus_tag="PN977_000406"
     CDS             13296..14165
                     /locus_tag="PN977_000406"
                     /EC_number="2.7.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribulokinase"
                     /protein_id="HDI6040487.1"
                     /translation="MSAKHPVIAVTGSSGAGTTTTSLAFRKIFAQLNLHAAEVEGDSF
                     HRYTRPEMDMAIRKARDAGRHISYFGPEANDFGLLEQTFIEYGQSGKGKSRKYLHTYD
                     EAVPWNQVPGTFTPWQPLPEPTDVLFYEGLHGGVVTPQHNVAQHVDLLVGVVPIVNLE
                     WIQKLIRDTSERGHSREAVMDSVVRSMEDYINYITPQFSRTHLNFQRVPTVDTSNPFA
                     AKGIPSLDESFVVIHFRNLEGIDFPWLLAMLQGSFISHINTLVVPGGKMGLAMELIML
                     PLVQRLMEGKKIE"
     gene            complement(14220..14624)
                     /locus_tag="PN977_000407"
     CDS             complement(14220..14624)
                     /locus_tag="PN977_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HDI6040488.1"
                     /translation="MQARVKWVEGLTFLGESASGHQILMDGNSGDKAPSPMEMVLMAA
                     GGCSAIDVVSILQKGRQDVVDCEVKLTSERREEAPRLFTHINLHFIVTGRDLKDAAVA
                     RAVDLSAEKYCSVALMLEKAVNITHSYEVVAA"
     gene            14926..15558
                     /gene="crp"
                     /locus_tag="PN977_000408"
     CDS             14926..15558
                     /gene="crp"
                     /locus_tag="PN977_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cAMP-activated global transcriptional regulator
                     CRP"
                     /protein_id="HDI6040489.1"
                     /translation="MVLGKPQTDPTLEWFLSHCHIHKYPSKSTLIHQGEKAETLYYIV
                     KGSVAVLIKDEEGKEMILSYLNQGDFIGELGLFEEGQERSAWVRAKTACEVAEISYKK
                     FRQLIQVNPDILMRLSAQMARRLQVTSEKVGNLAFLDVTGRIAQTLLNLAKQPDAMTH
                     PDGMQIKITRQEIGQIVGCSRETVGRILKMLEDQNLISAHGKTIVVYGTR"
     gene            15609..17699
                     /locus_tag="PN977_000409"
     CDS             15609..17699
                     /locus_tag="PN977_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312236.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HDI6040490.1"
                     /translation="MWRRLIYHPDINYALRQTLVLCLPVAVGLMLGELRFGLLFSLVP
                     ACCNIAGLDTPHKRFFKRLIIGASLFATCSLLTQVLLAKDVPLPFLLTGLTLVLGVTA
                     ELGPLHAKLLPASLLAAIFTLSLAGYMPVWEPLLIYALGTLWYGLFNWFWFWIWREQP
                     LRESLSLLYRELADYCEAKYSLLTQHTDPEKALPPLLVRQQKAVDLITQCYQQMHMLS
                     AQNNTDYKRMLRIFQEALDLQEHISVSLHQPEEVQKLVERSHAEEVIRWNAQTVAARL
                     RVLADDILYHRLPTRFTMEKQIGALEKIARQHPDNPVGQFCYWHFSRIARVLRTQKPL
                     YARDLLADKQRRMPLLPALKSYLSLKSPALRNAGRLSVMLSVASLMGTALHLPKSYWI
                     LMTVLLVTQNGYGATRLRIVNRSVGTVVGLIIAGVALHFKIPEGYTLTLMLITTLASY
                     LILRKNYGWATVGFTITAVYTLQLLWLNGEQYILPRLIDTIIGCLIAFGGTVWLWPQW
                     QSGLLRKNAHDALEAYQDAIRLILSEDPQPTPLAWQRMRVNQAHNTLYNSLNQAMQEP
                     AFNSHYLADMKLWVTHSQFIVEHINAMTTLAREHRALPPELAQEYLQSCEIAIQRCQQ
                     RLEYDEPGSSGDANIMDAPEMQPHEGAAGTLEQHLQRVIGHLNTMHTISSMAWRQRPH
                     HGIWLSRKLRDSKA"
     gene            complement(17762..18985)
                     /gene="argD/dapC"
                     /locus_tag="PN977_000410"
     CDS             complement(17762..18985)
                     /gene="argD/dapC"
                     /locus_tag="PN977_000410"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001765220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     acetylornithine/succinyldiaminopimelate transaminase"
                     /protein_id="HDI6040491.1"
                     /translation="MAIEQTAITRATFDEVILPIYAPAEFIPVKGQGSRIWDQQGKEY
                     VDFAGGIAVTALGHCHPALVNALKTQGETLWHISNVFTNEPALRLGRKLIEATFAERV
                     VFMNSGTEANETAFKLARHYACVRHSPFKTKIIAFHNAFHGRSLFTVSVGGQPKYSDG
                     FGPKPADIIHVPFNDLHAVKAVMDDHTCAVVVEPIQGEGGVTAATPEFLQGLRELCDQ
                     HQALLVFDEVQCGMGRTGDLFAYMHYGVTPDILTSAKALGGGFPISAMLTTAEIASAF
                     HPGSHGSTYGGNPLACAVAGAAFDIINTPEVLEGIQAKRQRFVDHLQKIDQQYDVFSD
                     IRGMGLLIGAELKPQYKGQARDFLYAGAEEGVMVLNAGPDVMRFAPSLVVEDADIDEG
                     MHRFAHAVAKVIGGK"
     gene            complement(19071..19634)
                     /gene="pabA"
                     /locus_tag="PN977_000411"
     CDS             complement(19071..19634)
                     /gene="pabA"
                     /locus_tag="PN977_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 2"
                     /protein_id="HDI6040492.1"
                     /translation="MILLIDNYDSFTWNLYQYFCELGADVLVKRNDALTLADIDALKP
                     QKIVISPGPCTPDEAGISLDVIRHYAGRLPILGVCLGHQAMAQAFGGKVVRTAKVMHG
                     KTSPITHNGEGVFRGLANPLTVTRYHSLVVEPDSLPACFDVTAWSETREIMGIRHRQW
                     DLEGVQFHPESILSEQGHQLLANFLHR"
     gene            complement(19666..20268)
                     /locus_tag="PN977_000412"
     CDS             complement(19666..20268)
                     /locus_tag="PN977_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative adenosine monophosphate-protein
                     transferase Fic"
                     /protein_id="HDI6040493.1"
                     /translation="MSDKFGEGRDPYLYPGLDIMRNRLNIHQQQRLEQAAYEMTALRA
                     ATIELGPLVRGLPHLRTIHRQLYQDIFDWAGQLREVDIYQGDTPFCHFAYIEKEGNAL
                     MQDLEEEGYLVGLEKAKFVERLAHYYCEINVLHPFRVGSGLAQRIFFEQLAIHAGYQL
                     SWQGIEKEAWNQANQSGAMGDLTALQTIFSKVVSEAGESE"
     gene            complement(20258..20425)
                     /locus_tag="PN977_000413"
     CDS             complement(20258..20425)
                     /locus_tag="PN977_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfG family protein"
                     /protein_id="HDI6040494.1"
                     /translation="MKKLTDKQKSRLWELQRNRNFQASRRLEGVEMPLVTLTATEALA
                     RLEALRRHYER"
     gene            complement(20530..21102)
                     /gene="ppiA"
                     /locus_tag="PN977_000414"
                     /gene_synonym="rotA"
     CDS             complement(20530..21102)
                     /gene="ppiA"
                     /locus_tag="PN977_000414"
                     /gene_synonym="rotA"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase A"
                     /protein_id="HDI6040495.1"
                     /translation="MFKSTLAAMAAVFALSALSPAAMAAKGDPHVLLTTSAGNIELEL
                     DKQKAPVSVQNFVDYVNSGFYNNTTFHRVIPGFMIQGGGFTEQMQQKKPNPPIKNEAD
                     NGLRNTRGTIAMARTADKDSATSQFFINVADNAFLDHGQRDFGYAVFGKVVKGMDVAD
                     KISQVPTHDVGPYQNVPSKPVVILSAKVLP"
     gene            21373..22554
                     /gene="tsgA"
                     /locus_tag="PN977_000415"
     CDS             21373..22554
                     /gene="tsgA"
                     /locus_tag="PN977_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter TsgA"
                     /protein_id="HDI6040496.1"
                     /translation="MTNSNRIKLTWISFLSYALTGALVIVTGMVMGNIADYFNLPVSS
                     MSNTFTFLNAGILISIFLNAWLMEIVPLKTQLRFGFLLMVLAVAGLMFSHSLALFSAA
                     MFILGVVSGITMSIGTFLVTQMYEGRQRGSRLLFTDSFFSMAGMIFPMIAAFLLARSI
                     EWYWVYACIGLVYVAIFILTFGCEFPALGKHAPKTDAPVAKEKWGIGVLFLSIAALCY
                     ILGQLGFISWVPEYAKGLGMSLNDAGTLVSNFWMSYMVGMWAFSFILRFFDLQRILTV
                     LAGLAAILMYVFNTGTPAHMAWSILALGFFSSAIYTTIITLGSQQTKVPSPKLVNFVL
                     TCGTIGTMLTFVVTGPIVEHSGPQAALLTANGLYAVVFVMCFLLGFVSRHRQHNTLTS
                     H"
     gene            22816..25359
                     /gene="nirB"
                     /locus_tag="PN977_000416"
     CDS             22816..25359
                     /gene="nirB"
                     /locus_tag="PN977_000416"
                     /EC_number="1.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-nitrite reductase large subunit"
                     /protein_id="HDI6040497.1"
                     /translation="MSKVRLAIIGNGMVGHRFIEDLLDKSDAANFDITVFCEEPRIAY
                     DRVHLSSYFSHHTAEELSLVREGFYEKHGIKVLVGERAITINRQEKVIHSSAGRTVFY
                     DKLIMATGSYPWIPPIKGSDTQDCFVYRTIEDLNAIESCARRSKRGAVVGGGLLGLEA
                     AGALKNLGIETHVIEFAPMLMAEQLDQMGGEQLRRKIESMGVRVHTSKNTLEIVQEGV
                     EARKTMRFADGSELEVDFIVFSTGIRPRDKLATQCGLDVAPRGGIVINDSCQTSDPDI
                     YAIGECASWNNRVFGLVAPGYKMAQVAVDHILGSENAFEGADLSAKLKLLGVDVGGIG
                     DAHGRTPGARSYVYLDESKEIYKRLIVSEDNKTLLGAVLVGDTSDYGNLLQLVLNAIE
                     LPENPDSLILPAHSGSGKPSIGVDKLPDSAQICSCFDVTKGDLIAAINKGCHTVAALK
                     AETKAGTGCGGCIPLVTQVLNAELAKQGIEVNNNLCEHFAYSRQELFHLIRVEGIKTF
                     EELLAKHGKGYGCEVCKPTVGSLLASCWNEYILKPEHTPLQDSNDNFLANIQKDGTYS
                     VIPRSPGGEITPEGLMAVGRIAREFNLYTKITGSQRLAMFGAQKDDLPEIWRQLIEAG
                     FETGHAYAKALRMAKTCVGSTWCRYGVGDSVGLGVELENRYKGIRTPHKMKFGVSGCT
                     RECSEAQGKDVGIIATEKGWNLYVCGNGGMKPRHADLLAADIDRETLIKYLDRFMMFY
                     IRTADKLTRTAPWLENLEGGIDYLKAVIIDDKLGLNAHLEEEMARLREAVVCEWTETV
                     NTPSAQTRFKHFINSDKRDPNVQMVPEREQHRPATPYERIPVTLVEDNA"
     gene            25356..25682
                     /gene="nirD"
                     /locus_tag="PN977_000417"
     CDS             25356..25682
                     /gene="nirD"
                     /locus_tag="PN977_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase small subunit NirD"
                     /protein_id="HDI6040498.1"
                     /translation="MSQWKDICKIDDILPETGVCALLGDEQVAIFRPYHSDQVFAISN
                     IDPFFESSVLSRGLIAEHQGELWVASPLKKQRFRLSDGLCMEDEQFSVKHYDARVKDG
                     VVQLRG"
     gene            25807..26613
                     /gene="nirC"
                     /locus_tag="PN977_000418"
     CDS             25807..26613
                     /gene="nirC"
                     /locus_tag="PN977_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite transporter NirC"
                     /protein_id="HDI6040499.1"
                     /translation="MFTDTINKCAANAARIARLSANNPLGFWVSSAMAGAYVGLGIIL
                     IFTLGNLLDPSVRPLVMGATFGIALTLVIIAGSELFTGHTMFLTFGVKAGTISHGQMW
                     AILPQTWLGNLVGSVFVAMLYSWGGGSLLPVDTSIVHSVALAKTTAPAMVLFFKGALC
                     NWLVCLAIWMALRTEGAAKFIAIWWCLLAFIASGYEHSIANMTLFALSWFGNHSEAYT
                     LAGIGHNLLWVTLGNTLSGAVFMGLGYWYATPKANRPVADKFNQTETAAG"
     gene            26632..28005
                     /gene="cobA"
                     /locus_tag="PN977_000419"
     CDS             26632..28005
                     /gene="cobA"
                     /locus_tag="PN977_000419"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HDI6040500.1"
                     /translation="MDHLPIFCQLRDRDCLIVGGGDVAERKARLLLDAGARLTVNALA
                     FIPQFTAWADAGMLTLVEGPFDESLLDTCWLAIAATDDDALNQRVSEAAEARRIFCNV
                     VDAPKAASFIMPSIIDRSPLMVAVSSGGTSPVLARLLREKLESLLPLHLGQVAKYAGQ
                     LRGRVKQQFATMGERRRFWEKLFVNDRLAQSLANNDQKAITETTEQLINEPLDHRGEV
                     VLVGAGPGDAGLLTLKGLQQIQQADVVVYDRLVSDDIMNLVRRDADRVFVGKRAGYHC
                     VPQEEINQILLREAQKGKRVVRLKGGDPFIFGRGGEELETLCNAGIPFSVVPGITAAS
                     GCSAYSGIPLTHRDYAQSVRLITGHLKTGGELDWENLAAEKQTLVFYMGLNQAATIQQ
                     KLIEHGMPGEMPVAIVENGTAVTQRVIDGTLTQLGELAQQMNSPSLIIIGRVVGLRDK
                     LNWFSNH"
     gene            28256..28423
                     /locus_tag="PN977_000420"
     CDS             28256..28423
                     /locus_tag="PN977_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfL family protein"
                     /protein_id="HDI6040501.1"
                     /translation="MNKFIKVALVGAVLATLTACTGHIENRDKNCSYDYLLHPAISIS
                     KIIGGCGPTAQ"
     gene            complement(28530..29615)
                     /locus_tag="PN977_000421"
     CDS             complement(28530..29615)
                     /locus_tag="PN977_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HDI6040502.1"
                     /translation="MKTFPLQSLTLIEAQQKQFALVDSICRHFPGSEFLAGGDLGLTP
                     GLNQPRITQRVEQVLADAFHAQAAALVQGAGTGAIRAALAALLKPGQRLLVHDAPVYP
                     TTQVIIEQMGLTLITANFNDLSALKQVVDEQQPDAALVQHTRQQPQDGYILADVLATL
                     RSAGVPALTDDNYAVMKVARIGCECGANVSTFSCFKLFGPEGVGAVVGDADVISRIRA
                     TLYSGGSQVQGAQALEVLRGLVLAPVMHAVQAGVSERLLALLNGGAVAEVKSAVIANA
                     QSKVLIVEFHQPIAARVLEEAQKLGALPYPVGAESKYEIPPLFYRLSGTFRQANPQLE
                     HCAIRINPNRSGEETVLRILRESIADV"
     gene            complement(29627..30931)
                     /locus_tag="PN977_000422"
     CDS             complement(29627..30931)
                     /locus_tag="PN977_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfT family protein"
                     /protein_id="HDI6040503.1"
                     /translation="MDLYIQIIVVACLTGMTSLLAHRSAAVFHDGIRPILPQLIEGYM
                     NRREAGSIAFGLSIGFVASVGISFTLKTGLLNAWLLFLPTDILGVLAINSLMAFGLGA
                     IWGVLILTCLLPVNQLLTALPVDVLGSLGELSSPVVSAFALFPLVAIFYQFGWKQSLI
                     AAVVVLMTRVVVVRYFPHLNPESIEIFIGMVMLLGIAITHDLRHRDENDIDASGLSVF
                     EERTSRIIKNLPYIAIVGALIAAVASMKIFAGSEVSIFTLEKAYSAGVTPEQSQTLIN
                     QAALAEFMRGLGFVPMIATTALATGVYAVAGFTFVYAVGYLSPNPMVAAVLGAVVISA
                     EVLLLRSIGKWLGRYPSVRNASDNIRNAMNMLMEVALLVGSIFAAIKMAGYTGFSIAV
                     AIYFLNESLGRPVQKMAAPVVAVMITGILLNVLYWLGLFVPA"
     gene            complement(30943..31296)
                     /locus_tag="PN977_000423"
     CDS             complement(30943..31296)
                     /locus_tag="PN977_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2620 domain-containing protein"
                     /protein_id="HDI6040504.1"
                     /translation="MKKIGVAGLQREQIKKTIEATAPGCFEVFIHNDMEAAMKVKSGQ
                     LDYYIGACNTGAGAALSIAIAVIGYNKSCTIAKPGIKAKDEHIAKMIAEGKVAFGLSV
                     EHVEHAIPMLINHLK"
     gene            complement(31307..32185)
                     /locus_tag="PN977_000424"
     CDS             complement(31307..32185)
                     /locus_tag="PN977_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotriesterase-related protein"
                     /protein_id="HDI6040505.1"
                     /translation="MSFDPTGYTLAHEHLHIDLSGFKNNVDCRLDQYAFICQEMNDLM
                     ARGVRNVIEMTNRYMGRNAQFMLDVMRETGINVVACTGYYQDAFFPEHVATRSVQELA
                     QEMVDEIEQGIDGTDLKAGIIAEIGSSEGKITSLEEKVFIAAALAHNQTGRPISTHTS
                     FSTMGLEQLALLQAHGVDLSRVTVGHCDLKDNLDNILKMIDLGAYVQFDTIGKNSYYP
                     DEKRIAMLHALRDRGLLNRVMLSMDITRRSHLKANGGYGYDFLLTTFIPQLRQSGFSQ
                     ADVDVMLRENPSQFFQ"
     gene            complement(32182..33408)
                     /locus_tag="PN977_000425"
     CDS             complement(32182..33408)
                     /locus_tag="PN977_000425"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HDI6040506.1"
                     /translation="MARFVVLVIDSFGVGAMKDVTLVRPQDAGANTCGHILSQLPHLQ
                     LPTLEKLGLINALGYAPGDMQPSDSATWGVAELQHEGGDTFMGHQEILGTRPLPPLRM
                     PFRDVIDRVEQALVSAAWQVERRGEELQFLWVNQAVAIGDNLEADLGQVYNITANLSV
                     VSFDDAIKIGRIVREQVPVGRVITFGGLLTDSQRILDAAESKEGRFIGINAPRSGAYD
                     NGFQVVHMGYGVDEKVQVPQKLYEAGVPTVLVGKVADIVNNPYGVSWQNLVDSQRIMD
                     ITLDEFITHQTAFICTNIQETDLAGHAEDVARYAERLQVVDRNLALLVETMQPDDCLV
                     VMADHGNDPTIGHSHHTREVVPVLVYQQGLAATQLGVRTTLSDVGATVCEFFRAPPPQ
                     NGRSFLSSLRFAGDTL"
     gene            complement(33408..34571)
                     /locus_tag="PN977_000426"
     CDS             complement(33408..34571)
                     /locus_tag="PN977_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfX family PLP-dependent enzyme"
                     /protein_id="HDI6040507.1"
                     /translation="MFVEALKRQNPALISAALSLWQKGKIAPDSWVIDVDQILENGKR
                     LIETARLYGIELYLMTKQFGRNPWLAEKLLALGYSGIVAVDYKEARVMRRAGLPVAHQ
                     GHLVQIPCHLVSDAVEQGTDVITVFTLDKAREVSAAALKTGRVQSVLLKVYSDDDFLY
                     PGQESGFALQVLPEIVAEIQNLPGLHLAGLTHFPCLLWDEAVGKVLPTPNLHTLVQAR
                     DQLAKSGIAIEQLNAPSATSCTSLPLLAEYGVTHAEPGHALTGTIPANQQGDQPERIA
                     MLWLSEISHHFRGDSYCYGGGYYRRGHAQHALVFTPENQKITETNLKTVDDSSIDYTL
                     PLAGEFPVSSAVVLCFRTQIFVTRSDVVLLSGIHRGEPEIVGRYDSLGNSLGA"
     gene            complement(34655..35017)
                     /locus_tag="PN977_000427"
     CDS             complement(34655..35017)
                     /locus_tag="PN977_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HDI6040508.1"
                     /translation="METRLNLLCDAGVIDKDICKGMMQVVNVLETECHLPVRSEQGMM
                     AMTHMASALMRSRRGEEIEPLDNELLAELAQSSHWQAVVQLHQVLLKEFALEVNPCEE
                     GYLLANLYGLWMAVNEEV"
     gene            complement(35034..35939)
                     /locus_tag="PN977_000428"
     CDS             complement(35034..35939)
                     /locus_tag="PN977_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6040509.1"
                     /translation="MRRTFIKKEGVVITTLARYLLGEKCGNRLKTIDELATECRSSVG
                     LTQAALKTLESSGAIRIERRGRNGSYLVEMDNKALLTHVDINNVVCAMPLPYTRLYEG
                     LASGLKAQFDGIPFYYAHMRGADIRVECLLNGVYDMAVVSRLAAESYLTQKGLCLALE
                     LGPHTYVGEHQLICRKGESANVKRVGLDNRSADQKIMTDVFFGDSDVERVDLSYHESL
                     QRIVKGDVDAVIWNVVAENELTMLGLEATPLTDDPRFLQATEAVVLTRVDDYPMQQLL
                     RAVVDKQALLAHQQRVVSGEQEPSY"
     gene            complement(36099..37103)
                     /gene="trpS"
                     /locus_tag="PN977_000429"
     CDS             complement(36099..37103)
                     /gene="trpS"
                     /locus_tag="PN977_000429"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HDI6040510.1"
                     /translation="MTKPIVFSGAQPSGELTIGNYMGALRQWVNMQDDYHCIYCIVDQ
                     HAITVRQDAQKLRKATLDTLALYLACGIDPEKSTIFVQSHVPEHAQLGWALNCYTYFG
                     ELSRMTQFKDKSARYAENINAGLFDYPVLMAADILLYQTNLVPVGEDQKQHLELSRDI
                     AQRFNALYGDIFKVPEPFIPKSGARVMSLLEPTKKMSKSDDNRNNVIGLLEDPKSVVK
                     KIKRAVTDSDEPPVVRYDVQNKAGVSNLLDILSAVTGQSIPELEKQFEGKMYGHLKGE
                     VADAVSGMLTELQERYHRFRNDEAFLQQVMKDGAEKASAHASRTLKAVYEAIGFVAKP
                     "
     gene            complement(37096..37854)
                     /gene="gph"
                     /locus_tag="PN977_000430"
     CDS             complement(37096..37854)
                     /gene="gph"
                     /locus_tag="PN977_000430"
                     /EC_number="3.1.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HDI6040511.1"
                     /translation="MNKFEDIRGVAFDLDGTLVDSAPGLATAVDMALYALELPVAGEE
                     RVITWIGNGADVLMERALTWARQERATLRKTMGKPPVDDDIPAEEQVRILRKLFDRYY
                     GEVAEEGTFLFPHVADTLGALQAKGLPLGLVTNKPTPFVAPLLEALDIAKYFSVVIGG
                     DDVQNKKPHPDPLLLVAERMGIAPQQMLFVGDSRNDIQAAKAAGCPSVGLTYGYNYGE
                     AIDLSQPDVIYQSINDLLPALGLPHSENQESKND"
     gene            complement(37847..38524)
                     /gene="rpe"
                     /locus_tag="PN977_000431"
     CDS             complement(37847..38524)
                     /gene="rpe"
                     /locus_tag="PN977_000431"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HDI6040512.1"
                     /translation="MKQYLIAPSILSADFARLGEDTAKALAAGADVVHFDVMDNHYVP
                     NLTIGPMVLKSLRNYGITAPIDVHLMVKPVDRIVPDFAAAGASIITFHPEASEHVDRT
                     LQLIKENGCKAGLVFNPATPLSYLDYVMDKLDVILLMSVNPGFGGQSFIPQTLDKLRE
                     VRRRIDESGFDIRLEVDGGVKVNNIGEIAAAGADMFVAGSAIFDQPDYKKVIDEMRSE
                     LAKVSHE"
     gene            complement(38542..39378)
                     /gene="dam"
                     /locus_tag="PN977_000432"
     CDS             complement(38542..39378)
                     /gene="dam"
                     /locus_tag="PN977_000432"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HDI6040513.1"
                     /translation="MKKNRAFLKWAGGKYPLLDDIKRHLPKGECLVEPFVGAGSVFLN
                     TDFSRYILADINSDLISLYNIVKMRTDEYVQAARELFVPETNCAEVYYQFREEFNKSQ
                     DPFRRAVLFLYLNRYGYNGLCRYNLRGEFNVPFGRYKKPYFPEAELYHFAEKAQNAFF
                     YCESYADSMARADDASVVYCDPPYAPLSATANFTAYHTNSFTLEQQAHLAEIAEGLVD
                     RHIPVLISNHDTMLTREWYQRAKLHVVKVRRSISSNGGTRKKVDELLALYKPGVVSPA
                     KK"
     gene            complement(39485..40771)
                     /gene="damX"
                     /locus_tag="PN977_000433"
     CDS             complement(39485..40771)
                     /gene="damX"
                     /locus_tag="PN977_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DamX"
                     /protein_id="HDI6040514.1"
                     /translation="MDEFKPEDELKPDPSDRRTGRSRQSSERSERTERGEPQINFDDI
                     ELDDTDDRRPTRAQKERNDEPEIEEEIDESEDEIVDEERVERRPRKRKKAASKPASRQ
                     YMMMGVGILVLLLLIIGIGSALKAPSTSSSDQTASGEKSIDLAGNATDQANGVQPAPG
                     TTSAENTQQDVSLPPISSTPTQGQTPAATDGQQRVEVQGDLNNALTQPQNQQQLNNVA
                     VNSTLPTEPATVAPVRNGNASRDTAKTQTAERPATTRPARQQAVIEPKKPQATVKTEP
                     KPVAQTPKRTEPAAPVASTKAPAATSTPAPKETATTAPVQTASPAQTTATPAAGGKTA
                     GNVGSLKSAPSSHYTLQLSSSSNYDNLNGWAKKENLKNYVVYETTRNGQPWYVLVSGV
                     YASKEEAKKAVSTLPADVQAKNPWAKPLRQVQADLK"
     gene            complement(40863..41951)
                     /gene="aroB"
                     /locus_tag="PN977_000434"
     CDS             complement(40863..41951)
                     /gene="aroB"
                     /locus_tag="PN977_000434"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HDI6040515.1"
                     /translation="MERIVVTLGERSYPITIASGLFNEPASFLPLKSGEQVMLVTNET
                     LAPLYLDKVRGVLEQAGVNVDSVILPDGEQYKSLAVLDTVFTALLQKPHGRDTTLVAL
                     GGGVVGDLTGFAAASYQRGVRFIQVPTTLLSQVDSSVGGKTAVNHPLGKNMIGAFYQP
                     ASVVVDLDCLKTLPPRELASGLAEVIKYGIILDGAFFNWLEENLDALLRLDGPAMAYC
                     IRRCCELKAEVVAADERETGLRALLNLGHTFGHAIEAEMGYGNWLHGEAVAAGMVMAA
                     RTSERLGQFSSAETQRIITLLTRAGLPVNGPREMSAQAYLPHMLRDKKVLAGEMRLIL
                     PLAIGKSEVRSGVSHELVLNAIADCQSA"
     gene            complement(42008..42529)
                     /gene="aroK"
                     /locus_tag="PN977_000435"
     CDS             complement(42008..42529)
                     /gene="aroK"
                     /locus_tag="PN977_000435"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004709337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroK"
                     /protein_id="HDI6040516.1"
                     /translation="MAEKRNIFLVGPMGAGKSTIGRQLAQQLNMEFYDSDQEIEKRTG
                     ADVGWVFDLEGEEGFRDREEKVINELTEKQGIVLATGGGSVKSRETRNRLSARGVVVY
                     LETTIEKQLARTQRDKKRPLLHVETPPREVLEALANERNPLYEEIADVTIRTDDQSAK
                     VVANQIIHMLESN"
     gene            complement(42930..44168)
                     /gene="hofQ"
                     /locus_tag="PN977_000436"
     CDS             complement(42930..44168)
                     /gene="hofQ"
                     /locus_tag="PN977_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA uptake porin HofQ"
                     /protein_id="HDI6040517.1"
                     /translation="MKQWIAALLLMLIPGVQAAKPQKVTLMVDDVPVAQVLQALAEQE
                     KLNLVVSPDVSGTVSLHLTDVPWKQALQTVVKSAGLITRQEGNILSVHSIAWQNDNIA
                     RQEAEQARAQANLPLENRNITLQYADAGELAKAGEKLLSAKGSMTVDKRTNRLLLRDN
                     KTALSALEQWVAQMDLPVGQVELSAHIVTINEKSLRELGVKWTLADAQQAGGVGQVTT
                     LGSDLSVATATTHVGFNIGRINGRLLDLELSALEQKQQLDIIASPRLLASHLQPASIK
                     QGSEIPYQVSSGESGATSVEFKEAVLGMEVTPTVLQKGRIRLKLHISQNVPGQVLQQA
                     DGEVLAIDKQEIETQVEVKSGETLALGGIFTRKNKSGQDSVPLLGDIPWFGQLFRHDG
                     KEDERRELVVFITPRLVSSE"
     gene            complement(44080..44484)
                     /gene="hofP"
                     /locus_tag="PN977_000437"
     CDS             complement(44080..44484)
                     /gene="hofP"
                     /locus_tag="PN977_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofP"
                     /protein_id="HDI6040518.1"
                     /translation="MRGKRWLLAGIALCLLTGMRDPFKPPEDLCRISELSQWRYQGIV
                     GRGERIIGVIKDGQKKWRRVQQNDVLENGWTILQLTPDALTLGTGTNCEPPQWLWQRQ
                     GDTNEAMDSRTTVDADTRRTGGKAAKSDTDGG"
     gene            complement(44474..44914)
                     /locus_tag="PN977_000438"
     CDS             complement(44474..44914)
                     /locus_tag="PN977_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofO"
                     /protein_id="HDI6040519.1"
                     /translation="MDMLFDWWFAASPRLRQLCWAFWLMMLVTLIFLSSTHQEESDAL
                     IRLRASHHQQWAALYRLVDTTSFSEEKTLPFSPLDFQLPGAQLVSWHPSAQGGELALK
                     TLWEAVPSAFTRLAERNVSVSRFSLSVEGDDLLFTLQLETPHEG"
     gene            complement(44898..45437)
                     /gene="hofN"
                     /locus_tag="PN977_000439"
     CDS             complement(44898..45437)
                     /gene="hofN"
                     /locus_tag="PN977_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofN"
                     /protein_id="HDI6040520.1"
                     /translation="MNPPINFLPWRQQRRTAFLRFWLLMFVAPLLLAVGITLIMRLTS
                     NAEARVNAVLLQAEQQLARSLQITKPRLLEQQQLREQRLQRQRQRQFTRDWQSALEAL
                     AALLPEHAWLTTISWQQGTLEIKGLTTSITALNALETSLRQDASFHLNQRGATQQDAQ
                     GRWQFEYQLTRKVSDGHAL"
     gene            complement(45437..46216)
                     /gene="hofM"
                     /locus_tag="PN977_000440"
     CDS             complement(45437..46216)
                     /gene="hofM"
                     /locus_tag="PN977_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000874542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofM"
                     /protein_id="HDI6040521.1"
                     /translation="MAFKIWQIGLHLQQQEAVAVAIVRGAKECFLQRWWRLPLENDII
                     KDGRIVDAQRLANTLLPWSRELPQRHHIMLAFPASRTLQRSFPRPSMSLGEREQTAWL
                     SGTMARELDMDPDSLRFDYSEDSLSPAYNVTAAQSKELATLLTLAERLRVHVSAITPD
                     ASALQRFLPFLPSHQQCLAWRDNEQWLWATRYRWGRKLAVGMTSAKELAAALSVDPES
                     VALCGEGGFDPWEAVSVRQPPLPPPGGDFAIALGLALGKAN"
     gene            46336..48888
                     /gene="mrcA"
                     /locus_tag="PN977_000441"
     CDS             46336..48888
                     /gene="mrcA"
                     /locus_tag="PN977_000441"
                     /EC_number="2.4.1.129"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase/peptidoglycan
                     DD-transpeptidase MrcA"
                     /protein_id="HDI6040522.1"
                     /translation="MKFVKYFLILAVCCILLGAGSIYGLYRYIEPQLPDVATLKDVRL
                     QIPMQIYSADGELIAQYGEKRRIPVTLDQIPPEMVKAFIATEDSRFYEHHGVDPVGIF
                     RAASVALFSGHASQGASTITQQLARNFFLSPERTLMRKIKEVFLAIRIEQLLTKDEIL
                     ELYLNKIYLGYRAYGVGAAAQVYFGKTVDQLTLNEMAVIAGLPKAPSTFNPLYSMDRA
                     VARRNVVLSRMLDEGYITQQQFDQTRTEAINANYHAPEIVFSAPYLSEMVRQEMYNRY
                     GESAYEDGYRIYTTITRKVQQAAQQAVRNNVLDYDMRHGYRGPANVLWKVGESAWDNN
                     KITDTLKALPTYGPLLPAAVTSANPQEATAMLADGSTVALSMEGVRWARPYRSDTQQG
                     PTPRKVTDVLQTGQQIWVRQVGDAWWLAQVPEVNSALVSINPQNGAVMALVGGFDFNQ
                     SKFNRATQALRQVGSNIKPFLYTAAMDKGLTLASMLNDVPISRWDAGAGSDWQPKNSP
                     PQYAGPIRLRQGLGQSKNVVMVRAMRAMGVDYAAEYLQRFGFPAQNIVHTESLALGSA
                     SFTPMQVARGYAVMANGGFLVDPWFISKIENDQGGVIFEAKPKVACPECDIPVIYGDT
                     QKSNVLENNDVEDVAISREQQNVSVPMPQLEQANQALVAKTGAQEYAPHVINTPLAFL
                     IKSALNTNIFGEPGWQGTGWRAGRDLQRHDIGGKTGTTNSSKDAWFSGYGPGVVTSVW
                     IGFDDHRRNLGHTTASGAIKDQISGYEGGAKSAQPAWDAYMKAVLEGVPEQPLTPPPG
                     IVTVNIDRSTGQLANGGNSREEYFIEGTQPTQQAVHEVGTTIIDNGEAQELF"
     gene            complement(49056..49616)
                     /gene="nudE"
                     /locus_tag="PN977_000442"
     CDS             complement(49056..49616)
                     /gene="nudE"
                     /locus_tag="PN977_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP compounds hydrolase NudE"
                     /protein_id="HDI6040523.1"
                     /translation="MSKSLQKPTILNVETVARSRLFTVESVDLEFSNGVRRVYERMRP
                     TNREAVMIVPIVDDHLILIREYAVGTESYELGFSKGLIDPGESVYEAANRELKEEVGF
                     GANDLTFLKKLSMAPSYFSSKMNIVVAQDLYPESLEGDEPEPLPQVRWPLAHMMDLLE
                     DPDFNEARNVSALFLVREWLKGQGRL"
     gene            49936..52071
                     /gene="igaA"
                     /locus_tag="PN977_000443"
     CDS             49936..52071
                     /gene="igaA"
                     /locus_tag="PN977_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intracellular growth attenuator protein IgaA"
                     /protein_id="HDI6040524.1"
                     /translation="MSTIVIFLAALLACSLLAGWLIKVRSRRRQLPWTNAFADAQTRK
                     LTPEERSAVENYLESLTQVLQVPGPTGASAAPISLALNAESNNVMMLTHAITRYGIST
                     DDPNKWRYYLDSVEVHLPPFWEQYINDENTVELIHTDSLPLVISLNGHTLQEYMQETR
                     GYALQPVPSTQASIRGEESEQIELLNIRKETHEEYALSRPRGLREALLIVASFLMFFF
                     CLITPDVFVPWLAGGALLLLGAGLWGLFAPPAKSSLREIHCLRGTPRRWGLFGENDQE
                     QINNISLGIIDLVYPAHWQPYIAQDLGQQTDIDIYLDRHVVRQGRYLSLHDEVKNFPL
                     QHWLRSTIIAAGSLLVLFMLLFWIPLDMPLKFTLSWMKGAQTIEATSVKQLADAGVRV
                     GDTLRISGTGMCNIRTSGTWSAKTNSPFLPFDCSQIIWNDARSLPLPESELVNKATAL
                     TEAVNRQLHPKPEDESRVSASLRSAIQKSGMVLLDDFGDIVLKTADLCSAKDDCVRLK
                     NALVNLGNSKDWDALVKRANAGKLDGVNVLLRPVSAESLDNLVATSTAPFITHETARA
                     AQSLNSPAPGGFLIVSDEGSDFVDQPWPSASLYDYPPQEQWNAFQKLAQMLMHTPFNA
                     EGIVTKIFTDANGTQHIGLHPIPDRSGLWRYLSTTLLLLTMLGSAIYNGVQAWRRYQR
                     HRTRMMKIQAYYESCLNPQLITPSESLIE"
     gene            52136..52804
                     /gene="yrfG"
                     /locus_tag="PN977_000444"
     CDS             52136..52804
                     /gene="yrfG"
                     /locus_tag="PN977_000444"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP/IMP nucleotidase"
                     /protein_id="HDI6040525.1"
                     /translation="MHINIAWQDVDTVLLDMDGTLLDLAFDNYFWQKLVPETWGAKNG
                     VTPQEAMEYMRQQYHDVQHTLNWYCLDYWSEQLGLDICAMTTEMGPRAVLREDTIPFL
                     EALKASGKQRILLTNAHPHNLAVKLEHTGLDAHLDLLLSTHTFGYPKEDQRLWHAVAE
                     ATGLKAERTLFIDDSEAILDAAAQFGIRYCLGVTNPDSGIAEKQYQRHPSLNDYRRLI
                     PSLM"
     gene            52815..53216
                     /gene="hslR"
                     /locus_tag="PN977_000445"
     CDS             52815..53216
                     /gene="hslR"
                     /locus_tag="PN977_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated heat shock protein Hsp15"
                     /protein_id="HDI6040526.1"
                     /translation="MKEKPAVEVRLDKWLWAARFYKTRALAREMIEGGKVHYNGQRSK
                     PSKIVELNATLTLRQGNDERTVIVKAITEQRRPASEAALLYEETAESVEKREKMALAR
                     KLNALTMPHPDRRPDKKERRDLLRFKHGDSE"
     gene            53241..54119
                     /gene="hslO"
                     /locus_tag="PN977_000446"
     CDS             53241..54119
                     /gene="hslO"
                     /locus_tag="PN977_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417860.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HDI6040527.1"
                     /translation="MPQHDQLHRYLFENFAVRGELVTVSETLQQILENHDYPQPVKNV
                     LAELLVATSLLTATLKFDGDITVQLQGDGPMNLAVINGNNNQQMRGVARVQGEIPENA
                     DLKTLVGNGYVVITITPSEGERYQGVVGLEGDTLAACLEDYFMRSEQLPTRLFIRTGD
                     VDGKPAAGGMLLQVMPAQNAQQDDFDHLATLTETIKTEELLTLPANEVLWRLYHEEEV
                     TVYDPQDVEFKCTCSRERCADALKTLPDEEVDSILAEDGEIDMHCDYCGNHYLFNAMD
                     IAEIRNNASPADPQVH"
     gene            complement(54253..55977)
                     /locus_tag="PN977_000447"
     CDS             complement(54253..55977)
                     /locus_tag="PN977_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4153 domain-containing protein"
                     /protein_id="HDI6040528.1"
                     /translation="MDSVELSPATRWGMIATGLLQGLVCYLLIAWLAGKNLSWIVYGV
                     PATVAFSSVLLFSVISFKQKRLWGWLALVFIATLGMSGWLKWQIDGMTPWRAEKAIWD
                     FGCYLLLMAMLLLPWIQQSLRIRNDSSRYRYFYQSVWHNVLILLVIFLANGLTWLVLL
                     LWSELFKLVGITFFKTLFFATDWFIYLTLGLVTALAVILARTQSRLIDSIQKLFTLIA
                     TGLLPLVSLLTLMFIITLPFTGLSAISRHISAAGLLLTLAFLQLILMAIVRDPQKASL
                     PWTGPLRCLIKTALLAAPLYVFVAAWALWLRVAQYGWTVDRLQGALAVLVLLVWSLGY
                     FVSIVWRKEQNPLVLQGKVNLAVSLLVLVILVLLNSPVLDSMRISVNSHMARYQSGKN
                     TPDQVTIYMLEQSGRYGRAALESLKSDAGFMKDPKRARDLLMALDGEQHLQEQVSEKV
                     LADNVLIAPGSGKPDATFWSALIQDRFNVMTCIEKDACVLVEQDLNSDGQAERILFAF
                     NDDRVIVYGFDSDRKEWDALDMSLLPRQITKEKLLAAAKDGKLGTRPKAWRDLTVDGE
                     TLEINLSK"
     gene            56356..57978
                     /gene="pckA"
                     /locus_tag="PN977_000448"
     CDS             56356..57978
                     /gene="pckA"
                     /locus_tag="PN977_000448"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HDI6040529.1"
                     /translation="MRVNNGLTPQELEAYGISDVHDIVYNPSYDLLYQEELDPSLTGY
                     ERGVLTNLGAVAVDTGIFTGRSPKDKYIVRDDTTRDTFWWADKGKGKNDNKPLSPETW
                     QHLKGLVTKQLSGKRLFVVDAFCGANPDTRLSVRFITEVAWQAHFVKNMFIRPSDEEL
                     AGFKPDFIVMNGAKCTNPQWKEQGLNSENFVAFNLTERMQLIGGTWYGGEMKKGMFSM
                     MNYLLPLKGIASMHCSANVGEKGDVAVFFGLSGTGKTTLSTDPKRRLIGDDEHGWDDD
                     GVFNFEGGCYAKTIKLSKEAEPEIYNAIRRDALLENVTVREDGSIDFDDGSKTENTRV
                     SYPIYHIENIVKPVSKAGHATKVIFLTADAFGVLPPVSRLTADQTQYHFLSGFTAKLA
                     GTERGITEPTPTFSACFGAAFLSLHPTQYAEVLVKRMQAAGAQAYLVNTGWNGTGKRI
                     SIKDTRAIIDAILNGSLDNAETFTLPMFNLAIPTELPGVDTKILDPRNTYASPEQWQE
                     KAETLAKLFIDNFDKYTDTPAGAALVAAGPKL"
     gene            58093..58410
                     /locus_tag="PN977_000449"
     CDS             58093..58410
                     /locus_tag="PN977_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HDI6040530.1"
                     /translation="MFTFIELQGFSKRRQLLLPDDEFRAFQELLIQDPTAGDIIAGTG
                     SFRKIRWNRSGMGKRGGTRIIYYNVTRNGRIYLALIYPKNEQDDLTEEQRKALKLLSE
                     KLL"
     gene            58469..58765
                     /locus_tag="PN977_000450"
     CDS             58469..58765
                     /locus_tag="PN977_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDI6040531.1"
                     /translation="MKDELFADLLASAEEMVRIEKGEETPEPEHVHTFSEIDVKAIRE
                     ATGLRQQDFAVAVGVSYDLVKSWETKRRQPTGAPRKLLLLLQANPFIINQLKTI"
     gene            complement(58797..60149)
                     /gene="envZ"
                     /locus_tag="PN977_000451"
     CDS             complement(58797..60149)
                     /gene="envZ"
                     /locus_tag="PN977_000451"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     EnvZ"
                     /protein_id="HDI6040532.1"
                     /translation="MRRLRFSPRSSFARTLLLIVTLLFVSLVTTYLVVLNFAILPSLQ
                     QFNKVLAYEVRMLMTDKLQLEDGTQLVVPPAFRREIYRELGISLYSNEAAEEAGLRWA
                     QHYEFLSHQMAQQLGGPTEVRVEVNKSSPVVWLKTWLSPNIWVRVPLTEIHQGDFSPL
                     FRYTLAIMLLAIGGAWLFIRIQNRPLVDLEHAALQVGKGIIPPPLREYGASEVRSVTR
                     AFNHMAAGVKQLADDRTLLMAGVSHDLRTPLTRIRLATEMMSEQDGYLAESINKDIEE
                     CNAIIEQFIDYLRTGQEMPMEMADLNAVLGEVIAAESGYEREIETALYPGSIEVKMHP
                     LSIKRAVANMVVNAARYGNGWIKVSSGTEPNRAWFQVEDDGPGIAPEQRKHLFQPFVR
                     GDSARTISGTGLGLAIVQRIVDNHNGMLELGTSERGGLSIRAWLPVPVTRAQGMTKEG
                     "
     gene            complement(60146..60865)
                     /gene="ompR"
                     /locus_tag="PN977_000452"
     CDS             complement(60146..60865)
                     /gene="ompR"
                     /locus_tag="PN977_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014646135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator OmpR"
                     /protein_id="HDI6040533.1"
                     /translation="MQENYKILVVDDDMRLRALLERYLTEQGFQVRSVANAEQMDRLL
                     TRESFHLMVLDLMLPGEDGLSICRRLRSQSNPMPIIMVTAKGEEVDRIVGLEIGADDY
                     IPKPFNPRELLARIRAVLRRQANELPGAPSQEEAVIAFGKFKLNLGTREMFREDEPMP
                     LTSGEFAVLKALVSHPREPLSRDKLMNLARGREYSAMERSIDVQISRLRRMVEEDPAH
                     PRYIQTVWGLGYVFVPDGSKA"
     gene            61093..61569
                     /gene="greB"
                     /locus_tag="PN977_000453"
     CDS             61093..61569
                     /gene="greB"
                     /locus_tag="PN977_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312275.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HDI6040534.1"
                     /translation="MKTPLVTREGYEKLKQELNYLWREERPEVTKKVTWAASLGDRSE
                     NADYQYNKKRLREIDRRVRYLTKCMENLKIVDYSPQQEGKVFFGAWVEIENDDGVTHR
                     FRIVGYDEIFGRKDYISIDSPMARALLKKEVGDLAVVNTPAGEASWYVNAIEYVKP"
     gene            61666..63987
                     /locus_tag="PN977_000454"
     CDS             61666..63987
                     /locus_tag="PN977_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HDI6040535.1"
                     /translation="MMNDSFCRIIAGEIQARPEQVDAAVRLLDEGNTVPFIARYRKEI
                     TGGLDDTQLRNLETRLSYLRELEERRQAILKSISEQGKLTDDLAKAINATLSKTELED
                     LYLPYKPKRRTRGQIAIEAGLEPLADLLWSDPSHTPEVAAAQYVDADKGVADTKAALD
                     GARYILMERFAEDAALLAKVRDYLWKNAHLVSTVVSGKEEEGAKFRDYFDHHEPLSTV
                     PSHRALAMFRGRNEGILQLSLNADPQFEEPPKESYCEQIIMDHLGLRLNNAPADSWRK
                     GVVSWTWRIKVLMHLETELMGTVRERAEDEAINVFARNLHDLLMAAPAGLRATMGLDP
                     GLRTGVKVAVVDATGKLVATDTIYPHTGQAAKAAMTVAALCEKHNVELVAIGNGTASR
                     ETERFYLDVQKQFPKVTAQKVIVSEAGASVYSASELAAQEFPDLDVSLRGAVSIARRL
                     QDPLAELVKIDPKSIGVGQYQHDVSQTQLARKLDAIVEDCVNAVGVDLNTASVPLLTR
                     VAGLTRMMAQNIVAWRDENGQFQNRQQLLKVSRLGPKAFEQCAGFLRINHGDNPLDAS
                     TVHPEAYPVVERILAATQQALKDLMGNSSELRNLKASDFTDEKFGVPTVTDIIKELEK
                     PGRDPRPEFKTAQFADGVETMNDLQPGMILEGAVTNVTNFGAFVDIGVHQDGLVHISS
                     LSNKFVEDPHTVVKAGDIVKVKVLEVDLQRKRIALTMRLDEQPGETNARRGGGNERPQ
                     NNRPAAKPRGREAQPAGNSAMMDALAAAMGKKR"
     gene            64425..64652
                     /gene="feoA"
                     /locus_tag="PN977_000455"
     CDS             64425..64652
                     /gene="feoA"
                     /locus_tag="PN977_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transporter A"
                     /protein_id="HDI6040536.1"
                     /translation="MQYTPDTAWKITGFSREISPAYRQKLLSLGMLPGSSFNVVRVAP
                     LGDPIHIETRRVSLVLRKKDLALLEVEAVSC"
     gene            64669..66990
                     /gene="feoB"
                     /locus_tag="PN977_000456"
     CDS             64669..66990
                     /gene="feoB"
                     /locus_tag="PN977_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(2+) transporter permease subunit FeoB"
                     /protein_id="HDI6040537.1"
                     /translation="MKKLTIGLIGNPNSGKTTLFNQLTGARQRVGNWAGVTVERKEGQ
                     FSTTDHQVTLVDLPGTYSLTTISSQTSLDEQIACHYILSGDADLLINVVDASNLERNL
                     YLTLQLLELGIPCIVALNMLDIAEKQNIRIEIDALSARLGCPVIPLVSTRGRGIEALK
                     LAIDRYKANENVELVHYAQPLLNEADSLAKVMPSDIPLKQRRWLGLQMLEGDIYSRAY
                     AGEASQHLDAALARLRNEMDDPALHIADARYQCIAAICDVVSNTLTAEPSRFTTAVDK
                     IVLNRFLGLPIFLFVMYLMFLLAINIGGALQPLFDVGSVALFVHGIQWIGYTLHFPDW
                     LTIFLAQGLGGGINTVLPLVPQIGMMYLFLSFLEDSGYMARAAFVMDRLMQALGLPGK
                     SFVPLIVGFGCNVPSVMGARTLDAPRERLMTIMMAPFMSCGARLAIFAVFAAAFFGQN
                     GALAVFSLYMLGIVMAVLTGLMLKYTIMRGEATPFVMELPVYHVPHVKSLIIQTWQRL
                     KGFVLRAGKVIIIVSIFLSAFNSFSLSGKIVDNINDSALASVSRVITPVFKPIGVHED
                     NWQATVGLFTGAMAKEVVVGTLNTLYTAENIQDEEFNPAEFNLGEELFSAIDETWQSL
                     KDTFSLSVLMNPIEASKGDGEMGTGAMGVMDQKFGSAAAAYSYLIFVLLYVPCISVMG
                     AIARESSRGWMGFSILWGLNIAYSLATLFYQVASYSQHPTYSLVCILAVILFNIVVIG
                     LLRRARSRVDIELLATRKSVSSCCAASTTGDCH"
     gene            66990..67226
                     /gene="feoC"
                     /locus_tag="PN977_000457"
     CDS             66990..67226
                     /gene="feoC"
                     /locus_tag="PN977_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-S]-dependent transcriptional repressor FeoC"
                     /protein_id="HDI6040538.1"
                     /translation="MASLIQVRDLLALRGRMEAAQISQTLNTPQPMINAMLQQLESMG
                     KAVRIQEEPDGCLSGSCKSCPEGKACLREWWALR"
     gene            67429..68307
                     /gene="rpnA"
                     /locus_tag="PN977_000458"
     CDS             67429..68307
                     /gene="rpnA"
                     /locus_tag="PN977_000458"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnA"
                     /protein_id="HDI6040539.1"
                     /translation="MSKKQSSTPHDALFKLFLRQPETARDFLAFHLPAPIHALCDMKT
                     LKLESSSFIDDDLRESYSDVLWSVKTEQGPGYIYCLIEHQSTSNKLIAFRMMRYAIAA
                     MQNHLDAGYKTLPMVVPLLFYHGIESPYPYSLCWLDCFADPKLARQLYASAFPLIDVT
                     VMPDDEIMQHRRMALLELIQKHIRQRDLMGLVEQMACLLSSGYANDRQIKGLFNYILQ
                     TGDAVRFNDFIDGVAEHSPKHKESLMTIAERLRQEGEQSKALHIAKIMLESGVPLADI
                     MRFTGVSEEELAAASQ"
     gene            complement(68336..69106)
                     /gene="bioH"
                     /locus_tag="PN977_000459"
     CDS             complement(68336..69106)
                     /gene="bioH"
                     /locus_tag="PN977_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pimeloyl-ACP methyl ester esterase BioH"
                     /protein_id="HDI6040540.1"
                     /translation="MNNIWWQTKGQGNVHLVLLHGWGLNAEVWRCIDEELSSHFTLHL
                     VDLPGFGRSRGFGAMSLADMAEAVLRQAPDKAIWLGWSLGGLVASQIALTHPERVQAL
                     VTVASSPCFSARDEWPGIKPDVLAGFQQQLSDDFQRTVERFLALQTMGTETARQDARA
                     LKKTVLALPMPEVDVLNGGLEILKTVDLRQPLQNVSMPFLRLYGYLDGLVPRKVVPML
                     DKLWPHSESYIFAKAAHAPFISHPAEFCRMLVALKQRV"
     gene            69144..69827
                     /gene="gntX"
                     /locus_tag="PN977_000460"
     CDS             69144..69827
                     /gene="gntX"
                     /locus_tag="PN977_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417872.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein GntX"
                     /protein_id="HDI6040541.1"
                     /translation="MLTVPGLCWLCRMPLALGHWGICSVCSLAARTDKTLCPQCGLPA
                     THSHLPCGRCLQKPPPWQRLVTVADYAPPLSPLIHQLKFSRRSEIASALSRLLLLEVL
                     HARRTTGLQLPDRIISVPLWQRRHWRRGFNQSDLLCQPLSRWLHCRWDSEAVTRTRAT
                     ATQHFLSARLRKRNLKNAFRLELPVQGRHMVIVDDVVTTGSTVAEIAQLLLRNGAATV
                     QVWCLCRTL"
     gene            69886..70461
                     /gene="nfuA"
                     /locus_tag="PN977_000461"
     CDS             69886..70461
                     /gene="nfuA"
                     /locus_tag="PN977_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016942932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S biogenesis protein NfuA"
                     /protein_id="HDI6040542.1"
                     /translation="MIRISDAAQAHFAKLLANQEEGTQIRVFVINPGTPNAECGVSYC
                     PPDAVEATDTALKFDLLTAYVDELSAPYLEDAEIDFVTDQLGSQLTLKAPNAKMRKVA
                     DDAPLMERVEYMLQSQINPQLAGHGGRVSLMEITEDGYAILQFGGGCNGCSMVDVTLK
                     EGIEKQLLNEFPELKGVRDLTEHQRGEHSYY"
     gene            70822..72138
                     /gene="gntT"
                     /locus_tag="PN977_000462"
     CDS             70822..72138
                     /gene="gntT"
                     /locus_tag="PN977_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HDI6040543.1"
                     /translation="MPLVIVAIGVILLLLLMIRFKMNGFIALVLVALAVGLMQGMPLD
                     KVIGSIKAGVGGTLGSLALIMGFGAMLGKMLADCGGAQRIATTLIAKFGKKHIQWAVV
                     LTGFTVGFALFYEVGFVLMLPLVFTIAASANIPLLYVGVPMAAALSVTHGFLPPHPGP
                     TAIATIFNADMGKTLLYGTILAIPTVILAGPVYARVLKGIDKPIPEGLYSAKTFSEEE
                     MPSFGVSVWTSLVPVVLMAMRAIAEMILPKGHAFLPVAEFLGDPVMATLIAVLIAMFT
                     FGLNRGRSMDQINDTLVSSIKIIAMMLLIIGGGGAFKQVLVDSGVDKYIASMMHETNI
                     SPLLMAWSIAAVLRIALGSATVAAITAGGIAAPLIATTGVSPELMVIAVGSGSVIFSH
                     VNDPGFWLFKEYFNLTIGETIKSWSMLETIISVCGLVGCLLLNMVI"
     gene            complement(72183..74267)
                     /gene="malQ"
                     /locus_tag="PN977_000463"
     CDS             complement(72183..74267)
                     /gene="malQ"
                     /locus_tag="PN977_000463"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HDI6040544.1"
                     /translation="MESKRLDNAALAAGISPNYINAHGKPQSISAETKRRLLDAMHQR
                     TATKVAVTPVPNVMVYTSGKKMPMVVEGSGEYSWLLTTEEGTQYKGHVTGGKAFNLPT
                     KLPEGYHTLTLTQDDQRAHCRVIVAPKRCYEPQALLNKQKLWGACVQLYTLRSEKNWG
                     IGDFGDLKAMLVDVAKRGGSFIGLNPIHALYPANPESASPYSPSSRRWLNVIYIDVNA
                     VEDFHLSEEALAWWKLPTTQQTLQQARDADWVDYSTVTALKMTALRMAWKGFAQRDDE
                     QVAAFRQFVAEQGDSLFWQAAFDALHAQQVKEDEMRWGWPAWPEMYQNVDSPEVRQFC
                     EEHRDDVDFYLWLQWLAYSQFAACWEISQDYEMPIGLYRDLAVGVAEGGAETWCDREL
                     YCLKASVGAPPDILGPLGQNWGLPPMDPHIITARAYEPFIELLRANMQNCGALRIDHV
                     MSMLRLWWIPYGETADQGAYVHYPVDDLLSILALESKRHRCMVIGEDLGTVPVEIVGK
                     LRSSGVYSYKVLYFENDHEKTFRSPKAYPEQSMAVAATHDLPTLRGYWESGDLTLGKT
                     LGLYPDEVVLRGLYQDRELAKQGLLDALHKYGCLPKRAGHKASLMSMTPTLNRGLQRY
                     IADSNSALLGLQPEDWLDMADPVNIPGTSYQYKNWRRKLSATLEAMFADDGVNKLLKD
                     LDRRRRAAAKKK"
     gene            complement(74277..76670)
                     /gene="malP"
                     /locus_tag="PN977_000464"
     CDS             complement(74277..76670)
                     /gene="malP"
                     /locus_tag="PN977_000464"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin phosphorylase"
                     /protein_id="HDI6040545.1"
                     /translation="MSQPIFNDKQFQEALSRQWQRYGLNSAAEMTPRQWWLAVSEALA
                     EMLRAQPFAKPVANQRHVNYISMEFLIGRLTGNNLLNLGWYQDVQDSLKAYDINLTDL
                     LEEEIDPALGNGGLGRLAACFLDSMATVGQSATGYGLNYQYGLFRQSFVDGKQVEAPD
                     DWHRSNYPWFRHNEALDVQVGIGGKVTKDGRWEPEFTITGQAWDLPVVGYRNGVAQPL
                     RLWQATHAHPFDLTKFNDGDFLRAEQQGINAEKLTKVLYPNDNHTAGKKLRLMQQYFQ
                     CACSVADILRRHHLAGRKLHELADYEVIQLNDTHPTIAIPELLRVLIDEHQMSWDDAW
                     AITSKTFAYTNHTLMPEALERWDVKLVKGLLPRHMQIINEINTRFKTLVEKNWPGDEK
                     VWAKLAVVHDKQVHMANLCVVGGFAVNGVAALHSDLVVKDLFPEYHQLWPNKFHNVTN
                     GITPRRWIKQCNPALAALLDKSLKKEWANDLDQLINLEKFADDAKFRQQYREIKQANK
                     VRLAEFVKVRTGIEINPQAIFDIQIKRLHEYKRQHLNLLHILALYKEIRENPQADRVP
                     RVFLFGAKAAPGYYLAKNIIFAINKVADVINNDPQVGDKLKVVFLPDYCVSAAEKLIP
                     AADISEQISTAGKEASGTGNMKLALNGALTVGTLDGANVEIAEKVGEENIFIFGHTVE
                     QVKAILAKGYDPVKWRKKDKVLDAVLKELESGKYSDGDKHAFDQMLHSIGKQGGDPYL
                     VMADFAAYVEAQKQVDVLYRDQEAWTRAAILNTARCGMFSSDRSIRDYQARIWQAKR"
     gene            77293..79998
                     /gene="malT"
                     /locus_tag="PN977_000465"
     CDS             77293..79998
                     /gene="malT"
                     /locus_tag="PN977_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MalT"
                     /protein_id="HDI6040546.1"
                     /translation="MLIPSKLSRPVRLDHTVVRERLLAKLSGANNFRLALITSPAGYG
                     KTTLISQWAAGKNDIGWYSLDEGDNQQERFASYLIAAVQQATNGHCAICETMAQKRQY
                     ASLTSLFAQLFIELAEWHSPLYLVIDDYHLITNPVIHESMRFFIRHQPENLTLVVLSR
                     NLPQLGIANLRVRDQLLEIGSQQLAFTHQEAKQFFDCRLSSPIEAAESSRICDDVSGW
                     ATALQLIALSARQNTHSAHKSARRLAGINASHLSDYLVDEVLDNVDLATRHFLLKSAI
                     LRSMNDALINRVTGEENGQMRLEEIERQGLFLQRMDDTGEWFCYHPLFGNFLRQRCQW
                     ELAAELPEIHRAAAESWMAQGFPSEAIHHALAAGDALMLRDILLNHAWSLFNHSELSL
                     LEESLKALPWDSLLENPQLVLLQAWLMQSQHRYGEVNTLLARAEHEIKDIREGTMHAE
                     FNALRAQVAINDGNPDEAERLAKLALEELPPGWFYSRIVATSVLGEVLHCKGELTRSL
                     ALMQQTEQMARQHDVWHYALWSLIQQSEILFAQGFLQTAWETQEKAFQLINEQHLEQL
                     PMHEFLVRIRAQLLWAWARLDEAEASARSGIEVLSSYQPQQQLQCLAMLIQCSLARGD
                     LDNARSQLNRLENLLGNGKYHSDWISNANKVRVIYWQMTGDKAAAANWLRHTAKPEFA
                     NNHFLQGQWRNIARAQILLGEFEPAEIVLEELNENARSLRLMSDLNRNLLLLNQLYWQ
                     AGRKSDAQRVLLDALKLANRTGFISHFVIEGEAMAQQLRQLIQLNTLPELEQHRAQRI
                     LREINQHHRHKFAHFDENFVERLLNHPEVPELIRTSPLTQREWQVLGLIYSGYSNEQI
                     AGELEVAATTIKTHIRNLYQKLGVAHRQAAVQHAQKLLKMMGYGV"
     gene            complement(80041..81057)
                     /gene="rtcA"
                     /locus_tag="PN977_000466"
     CDS             complement(80041..81057)
                     /gene="rtcA"
                     /locus_tag="PN977_000466"
                     /EC_number="6.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 3'-terminal phosphate cyclase"
                     /protein_id="HDI6040547.1"
                     /translation="MKRMIALDGAQGEGGGQILRSALSLSMITGLPFTITGIRAGRAK
                     PGLLRQHLTAVKAAAEICRATVEGAELGSQRLLFRPGTVRGGDYRFAIGSAGSCTLVL
                     QTVLPALWFADGPSRVEVSGGTDNPSAPPADFIRRVLEPLLAKMGIHQQTTLIRHGFY
                     PAGGGVVATEVSPVALFNTLQLGERGNIVQMRGEVLLAGVPRHVAEREIATLVGSFSL
                     HEQNIHNLPRDQGPGNTVSLEVESENITERFFVVGEKRVSAEVVAAQLVKEVKRYLAS
                     PAAVGEYLADQLVLPMALAGAGEFTVAHPSCHLLTNIAVVERFLPVRFGLVEADGVTR
                     VSIE"
     gene            complement(81061..82287)
                     /gene="rtcB"
                     /locus_tag="PN977_000467"
     CDS             complement(81061..82287)
                     /gene="rtcB"
                     /locus_tag="PN977_000467"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-splicing ligase RtcB"
                     /protein_id="HDI6040548.1"
                     /translation="MNYELLTTENVPVKMWTKGVPVEDDARQQLINTAKMPFIFKHIA
                     VMPDVHLGKGSTIGSVIPTKGAIIPAAVGVDIGCGMNALRTALTAADLPENLAELRQA
                     IEAAVPHGRTTGRCKRDKGAWEKPPVNVDAKWAELEAGYQWLTQKYPRFLNTNNYKHL
                     GTLGTGNHFIEICLDESDQVWIMLHSGSRGIGNAIGTYFIDLAQKEMQETLETLPSRD
                     LAYFMEGTEYFDDYLKAVAWAQLFASLNRDAMMENVVTALQSVTQKTVRQPQTLAMEE
                     INCHHNYVQKEQHFSEEIYVTRKGAVSARAGQYGIIPGSMGAKSFIVRGLGNEESFCS
                     CSHGAGRVMSRTKAKKLFSVEDQIRATAHVECRKDAEVIDEIPMAYKDIDAVMAAQSD
                     LVEVIYTLRQVVCVKG"
     gene            82475..>83041
                     /locus_tag="PN977_000468"
     CDS             82475..>83041
                     /locus_tag="PN977_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312292.1"
                     /note="sigma 54-dependent; regulates genes involved in
                     forming a 2',3'-cyclic phosphodiester on RNA; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-dependent transcriptional regulator"
                     /protein_id="HDI6040549.1"
                     /translation="MRKTVAFGFVGTVLDYAGRGSQRWSKWRPSLCLCQQESLVINRL
                     ELLHDARSRSLFETLKRDIANVSPETKVVGVEIELHNPWDFEEVYACLHDFARGYAFE
                     PDKEDYLIHITTGTHVAQICWFLLAEARYLPARLIQSSPPRKKEQPRGAGEVTIIDLD
                     LSRYNAIASRFAEERQQTLDFLKSGIATR"
BASE COUNT        19974 a        22595 c        20845 g        19627 t
ORIGIN      
        1 cgagcagcac cgccgtaggt tttagccagt acggtggtga ttgcagcggt cagagtagtt
       61 ttaccgtggt caacgtggcc gatagtacca acgttaacgt gcggttttgt acgttcaaat
      121 ttttctttag acacggctat attccttact atagtgctct ccccttcagg agagagcacg
      181 ggactttggt attaaccctt aggcttattt accacgggct tcaattacgg cctgagcaac
      241 gttactcggc gcttcatcat acttcaggaa ttccatggtg tatgatgcac gacctttggt
      301 cagagaacgc agctgagttg catatccgaa catttcagac agcggtactt cagcgtggat
      361 cttaacgcca gtaacttcag attcctgacc tttgagcata ccacgacgac ggctcaagtc
      421 accgataacg tcaccggtgt tctcttccgg agtttctact tcaaccttca tgatcggctc
      481 aagcagaact ggtttcgctt tcttaaagcc ttctttaaag gcgatagaag cagccagttt
      541 aaacgccagt tcagaggagt caacgtcatg gtaagaaccg aagtgcagac gaatacccat
      601 gtctactacc gggtagcctg ccagcggacc tgctttcagc tgttcctgga tacctttatc
      661 aacggccggg atgtattcgc cagggattac accaccttta atgtcgttga tgaactcgta
      721 gcctttcggg tttgaacccg gctccagcgg gtacatgtcg ataacaacat gaccatactg
      781 accacgacca ccagactgtt tcgcgtgttt accttcaaca tcggtaactt tctggcggat
      841 agtttcacgg taagcaacct gcggtttacc tacgttcgct tcaacgttga attcacgctt
      901 catacggtca acgatgatgt cgaggtgcag ttcgcccata cccgcgatga tggtctggtt
      961 agattcttcg tcagtccata cacggaaaga cgggtcttct ttagccagac ggcccagagc
     1021 cagacccatt ttttcctggt cagctttggt tttcggttca actgcgatgg agattaccgg
     1081 ctcagggaat tccatacgtt ccagaatgat cggcgcatcc gggtcacaca gggtgtcacc
     1141 agtggttacg tctttcagac cgattgcagc agcgatgtcg cccgcgcgaa cttctttgat
     1201 ctcttcacgt ttgttagcgt gcatctgaac gatacgaccg aaacgctcac gtgcagcttt
     1261 cacggagttc agtacggtat caccagagtt aaccacaccg gagtaaacac ggaagaaggt
     1321 caggttacca acaaacgggt cagtagcgat tttgaacgcc agtgcagaga acggctcgtc
     1381 atcacttgcg tgacgttcag ccggagtatc tttaccgtcg tccaggatac cgttgatcgc
     1441 aggtacgtca accggggatg gcaggtaatc aattaccgca tccagcatcg cctgaacacc
     1501 tttgttcttg aacgcagaac cacaggttac caggatgatt tcgttgttca gaacgcgctg
     1561 acgcagagca cctttgattt ctgcttcagt cagttcttca ccacccaggt atttttccat
     1621 cagctcttca gaagcttcag ctgcggattc gatcaggttc tggtgccatt cgttagccag
     1681 ttcaaccatg tctgccggga tatcttcgta ttcgaaggtt acgccctggt cagcgtcgtt
     1741 ccagttgata gctttcattt tcaccaggtc aacaacaccg gtgaaatgtt cttcagcacc
     1801 aatcgccagc tgcagcggaa ccgggttcgc gcccagacgg gttttgatct ggttaacaac
     1861 tttcaggaag ttcgcaccca tgcggtccat tttgttaacg aacgcaatgc gcggaacttt
     1921 atatttgttt gcctgacgcc atacggtttc agactgcggc tgaacaccac caactgcgca
     1981 gtaaaccatt accgcaccat cgagaacacg catggaacgt tctacttcga ttgtgaagtc
     2041 aacgtgcccc ggggtgtcga tgatgttgat gcgatgcggc tcatactgct tagccatacc
     2101 agaccagaat gcagtagtcg cagcggaagt gatggtaata ccacgttcct gctcctgctc
     2161 catccagtcc atggttgcag cgccgtcatg aacttcaccg attttatggt ttacaccggt
     2221 gtagaacaga atacgttcgg tagtagtggt tttaccggcg tcgatgtgcg cactgatacc
     2281 gatgttacgg tagcgtgcga tgggtgttgt acgagccatt tgtttcctcg tttatctttt
     2341 aggcgttcaa tttaagtagc ccaaagcggg ctgcttactg gaagcgcccg cctggtgact
     2401 aaaactccga agggattacc aacggtagtg tgcgaacgcc ttgttggctt cggccatacg
     2461 gtgaacgtct tcacgtttct taactgcagt acctttgttt tctgcagcat cagaaagttc
     2521 gttcgccagg cgcagagcca tggatttatc accgcgttta cgagcagctt caacgatcca
     2581 acgcattgcc agagcattac gacgaaccgg acggacttca actggtacct gataagtaga
     2641 accaccaacg cggcgagact taacttctac agtcgggcgc acgttttcga gagctacttc
     2701 gaatgcttcc agttcagatt taccagagcg ctgagccagg gtctccagcg cgctgtatac
     2761 gatagattca gcagtagatt ttttaccatc taccatcagg atatttacaa atttagccag
     2821 cagttctgat ccgaacttcg gatccggcag aattttacgc tgaccaatga cgcgacgacg
     2881 tggcatggaa atactccgtt gttaattcag gattgtccaa aactctacga gtttagtttg
     2941 acatttaagt taaaacgttt ggccttactt aacggagaac cattaagcct taggacgctt
     3001 cacgccatac ttggaacgag cctgcttacg gtctttaacg ccggagcagt caagcgcacc
     3061 acgtacggtg tggtaacgaa cacccgggag gtctttaaca cgaccgccac ggatcaggat
     3121 cacggagtgt tcctgcaggt tgtgaccttc accaccgatg taggaagtca cttcgaaacc
     3181 gttagtcaga cgaacacggc atactttacg cagcgcggag ttcggttttt taggagtggt
     3241 agtatataca cgagtacata cgccacgttt ttgcgggcat gcttccagcg caggcacgtt
     3301 gcttttcgca actttgcgag cacgtggttt gcgtaccagc tggttaactg ttgccattaa
     3361 atagctcctg gttttagctt ttgcttcgta aacacgtaat aaaacgtcct cacacaatat
     3421 gaggacgccg aattttaggg cgatgccgaa aaggtgtcaa gaaatataca acgatcccgc
     3481 catcaccagg ccatctggct ggagtgctta accgtaagtc tgacgaaatc agtatagtca
     3541 atgggaatga tgtcgttcga aattcgacca gtcaaaccgc gggcaataag gtcttcgttc
     3601 agggcataga ccttaatggg ggcattacgc agactttcaa ggtagcagtt accgtcaact
     3661 ccggcagtta cgccatcttg caataatagc agttcgtcgc cttcactgag cagacgcagc
     3721 agcgcagcaa aatccgtcag ccagggtgaa cgatgtaatg tatgcagcat aaacgcctca
     3781 aaacctcaaa ataacatcgt agttggcgag ttcgcggcgt aaggcatctg cttcgagcgg
     3841 cgtggcttcg acaacaaagg gtgtctgcgg atctaacccg cgttcgcgca gtgaagccgc
     3901 acaaacccag cactgttcaa tgtcgtacag acccaacaat ttaaaagtgg caatgtaatc
     3961 acgcgccagc actgcatcgg gcttttgtcc tggcagcagc tgaaaaacgc catcagctat
     4021 aaagaagaca gccagatcgt cagttaatgc ggaagttgcc agtaaagcat ctaaaccttc
     4081 ccggcctgca gctgtaccat gaggtgcagt agaaaaaaca aacgcaattc gtttcatcag
     4141 aactgtacca ccctgtcaca ggtcagcgag gcttccgcca gcgcgccaag tccgcttaag
     4201 gtaaatcccg actgaaggtt tgacgaagcc agccccagcc ttcctgcttc agtttcatcg
     4261 acgataccgc ggcgtaacgc tgccgctacg cagatattca gcgccacacc atgttgcgca
     4321 ttcaactgtt gccagccgcg cacaaggtca aattcatcgc ttgccggtga ggtcaattgg
     4381 ttagcgttat agaccccttc ccgatagaaa aagacgcagc ttaactcatg gccttcggct
     4441 atcagtgcct gcgcaaactg aaaagcacta ctcgcctgtt gcgtaccgta tgctggcccg
     4501 gtcaccacga tggcaaaacg cattacttat cttgcccctg gaaatcaccg ctcttgaact
     4561 ggcggatgta gagatagaca gtgtgtttgg agatgttcag gcggtcagca acctggttga
     4621 tcgcatcttt aatatcgaag atccctttct cgtagagatt cagcacgatc tgacgatttt
     4681 tggcgttatt agaaacattg cgatcggcat tcacttcttc gatggtgaac tccagcgttt
     4741 gggtaaccag atcttcaaca gaagaggcaa agttgacgct tgaaccgaca tccggggttt
     4801 ctggcggcac aaaggtgctc ataatctgcg agaagggaac atcaagattc atattgatgc
     4861 acagcagacc aattacacgc tgttcgcggt tacgaatcgc gatagtcagg gacttcatta
     4921 atacgccgct tttggcgcga gtaaagtagc atttagaaac gctgctatcc gctcccgtca
     4981 tatcgtgcag catacgtagc gccaggtcag taattggcga accaatcttc cggcctgtat
     5041 gttcaccgtt agcaatgcga atggctgaac attttagatc ctgcaaagag tgcaaaacga
     5101 tttcacagtg ggagccaata agcatcgcta acccgtccac caccgcttcg taggatttca
     5161 gaatatcaaa atcggtctgg tcgaaaggac gttgatccag taaatccaac tcactggttt
     5221 cgttggttaa aagcgacctg gacatgaaaa aaaacactcc ttttcaggag cctgtcgtta
     5281 acttttcagg gcaggctcat taatgatgcg ggtaactaaa ttaatacagc ggaggttccg
     5341 ctttccagca ctaattatat ccggcctgta ataaaaaaac cgccgcctgg tcaggcggcg
     5401 gttcttaatg cttatttttt agcagaatct gcggctttcg catcagcttc cggctttgca
     5461 tcagccttcg gcgctggttt cacatccagc agctctacgt caaacaccag ggtagaattc
     5521 ggtgggatcc ccggaacacc cgctttgccg taagccagtt ctggtggaat aaccagtttg
     5581 atcttaccgc ctttcttgat gttcttcaga ccttctgtcc aacccggaat aacaccgtcc
     5641 agacggaaag agagcggttc accacgggtg taagagttgt cgaactcttt tccgtcgatc
     5701 agcgtaccct tgtagttcac gacaacagta tcgctgtctt tcggtgcttc gcctttaccg
     5761 gcttctacta cctgataaac cagaccagtt gaagaggttt tcacaccttt ctctttggca
     5821 aatttctcgc ggtactcttt acctttcgct tcgttatctg ccgcgtcttt ttccatcttc
     5881 gcctgagcag aagacttcac gcgagcttcg aatgcttgca gagtctgttc gatctcttgg
     5941 tcggaaagtt tgctcttatc agcaaatgca tcctgaacac cagcgatcag ctgatcttta
     6001 tccagtttga tgcccagttt ttcttgttct ttaagagagt tttccatgta acgacccagc
     6061 gaagcaccca gtgcataagc tgatttctga tcgtcatttt tgaacgctgc tttgctgtca
     6121 gcagttgtag caggttttgc agcttcagca gcaaaagtga ttggtgcatg cagggcaacg
     6181 gccattgtgg tcgccagcag cgttacttta aacagtgatt tcatccatat ctccaggatc
     6241 ggggcatctc accccagggt taactattat cagaagggta ctataaagcg ttgtcgaaca
     6301 aatctacata cagacacgcc ctattatcat ctattttcag actctttttg tttaaattag
     6361 tttcgatgac cgcgaaatga gtgctgtctc gggcggcaaa gttaagtaga atccgcggca
     6421 accattcgac aaaagaggtg aatcatgcag gatttatcat tggaagcacg cctggcagag
     6481 ctggagagcc gactggcttt tcaggaaatc accattgaag aactgaacgt cacggtgacc
     6541 gctcatgaaa tggagatggc gaaactgcgc gatcatctgc gtctgctgac cgagaagcta
     6601 aaagccagcc agccgtcgaa catcgcgtcg caggctgaag aaacgccacc gccacattat
     6661 tgaggcgtaa aaaaagcggg gattcccccg cttttttgtc actttttcgg tattagtggc
     6721 aaccgcaacc gccgttacct ttaccgccgc agcagccttc gccaccgtgt tcatgaccgt
     6781 gatcgtggcc atgaccgccg cagcaaccgt cgtggtcgtg atcgtggtga tgatcgtgcg
     6841 cgccgtgaac gtgaccatga gccagttctt cttcggtcgc ttcgcgaatc gccacaactt
     6901 caacgttgaa tttcaggttc tgaccagcca gcatgtggtt accatcaacc actacgtgat
     6961 cgtcttcaac cgcagtgatt tcaaccggta ccggaccctg gtcggtttca gccaggaaac
     7021 gcatacctac ctgcagttca tcaacgccca taaatacgtc tttaggaacg cgttgcacca
     7081 ggttttcgtc gtactgaccg taagcgtcgt tcgcgccaac agcgacatca aatttgtcgc
     7141 caacttcgtg accttccagc gccgtttcca ggccagagat cagggaaccg tgaccatgca
     7201 ggtagtccag cggcgcactc accggagact catcaaccaa cacaccgtct tctgtacgta
     7261 cctgataggc caggctgacc accaggtctt ttgctacttt catgatatct cctgagcatg
     7321 ggaagaatag tggcgcagat tgtagcggaa ttctgcagcc gtgtactcac tagcttaaaa
     7381 aaactcggcg catatcgcta gtccggatga aaaatcccga tcacttgctc atctttgcga
     7441 acgtgatcgc gggcttcttt gtctgcttct cgcatctgat gtccgcactt aacacattca
     7501 acaatatcaa tattattttc gcgccacatc gccattgaat cctgcgcctg acaggccggg
     7561 cattttgccc ccgcaataaa acgttttcgg attgccatct ttaccctcta ctcaaattca
     7621 tcccagccgt ccagctggcg tcgttcttgt tgcatttcac gctggaaaat ctcttccagt
     7681 tcgcgtcggg cttccctggc gcgagaaatt tgtacggtat cggcatgcat tgggatgagc
     7741 tctcgcagca tccgcatatc caggcggcga aaatgcagtt gcgcccgctg tgcctgatgc
     7801 ggatgcatgc caagcgtgac cagcgtcttg cgccccagct ctaacgcact ggagaatgtt
     7861 tcacgggaaa actgcgtcac ccctgcctgt aataactcat gcgcttccac acgtccgcgc
     7921 gctcgcgcaa gaatatgcaa atgcggaaag tgctgctggc atatttccac cagtttcatg
     7981 gtgtcttccg gctcgttaca ggtaatgacg atagactcag cggcctctgc acccgcagaa
     8041 cgtaaaagat ctacctgcgt ggcgtcgccg taataaactt tgtagccata tttgcgcatc
     8101 aggttaacgg cactgatatc ccgctccagt acagtaatgc gcattttatt tgccatcagc
     8161 aaacgaccaa tcacctgacc aaaacgccca aagcccacga caatgacctg gggtttatca
     8221 tcgttgaccc atggtttttc gtcttcttct tccggtccgt taaactggcg ggatagccat
     8281 ttatctacca gcttcatcag cagcggcgtg gtcatcatgg aaagcgtcac cgtcaccagc
     8341 aacagcgcca tctggtcgcc ctggaataag cgttgtgaag aagcggtaga aaagaggaca
     8401 aaggcaaact caccgccctg actcaacacg ccagcaaact gcatccgctc tgagctacgc
     8461 acgccataca atcgcgccag cagatacagc acgagaattt tcaccgccac cagcacaacc
     8521 acacttatca ctacccacaa cagatgggta taaagcaccc cgaggttgag tgacatgccg
     8581 acagagataa agaacaaacc gagcagcaag cctttgaagg gatcgatagc cgtttccagt
     8641 tcatggcgat attcactttc cgccagtagc acacccgcaa taaacgtacc gagcgccatc
     8701 gacaacccca gcgcatccat aaacaatgcg gaacccaaca ccagcagcag cgtcgcggcg
     8761 gtgaacactt cccgcacacc agaagctgca ataaaacgga ataccggacg cagtaaatag
     8821 cgcccgccaa tcagcatgcc gacaaacgcc agcaccttca tgccgatctt catccagtcg
     8881 aaatgttcgt ctgccgaacc cgccaacaac ggcactaacg ccagtgccgg gatcaccgcc
     8941 agatcctgaa acagcagtac cgagaaacct aactggccgg attcgctgcg attcatcctt
     9001 ttatcacgca tcaattgcaa cgccattgca gttgaagaca tcgcaaggcc aatgccaccg
     9061 accaccgccg cctgccatga gaaatgggtc agcatcaata atcccgccag caacgccgcg
     9121 cttaacagca cctgtgccgc gcctacgcca aaaatcgaac gccgcaattg ccaaagcttg
     9181 gagggattca actccaggcc gatgataaac atcaggaaca ccacgccgag ttccgaaaag
     9241 tggaggatct catcgacgtc gctaataaaa cccagccccc acgggccaat tgcaatccct
     9301 gccagcaaat atcccaacac agcgccaata cccagccgcg atgccagcgg caccgcagcc
     9361 accgccgcga agagaaacaa cactcctgcg agtaaaaaat cggaaccttc catcagcggc
     9421 ctcctggaga cagcggattt gccagccagt caccgtaggc tctggcgtga ctcgccagct
     9481 cctgtgcgct ttgccgtctc gcccagtaaa taatgatggg actcaaccaa tgcatccggc
     9541 acatgcccgc cgccagttca aaggggcgca gcacatcgct catcgggtag cgattcagcg
     9601 cgtcataacg gtaagcactt tccggctcgc cggtggtaat cacgctacgc cagtactttc
     9661 ccgccagttg gtttcccccc ggtccgctgg caaaaccacg gcttaatacc cggtccagcc
     9721 actctttcag tagcgccggg cagctatagg tataaagagg atgctgaaag acaatcacct
     9781 cgtgctcgcg cagtaatgcc tgctcacgag ggatatcaat aaaaaagtcg ggatagtgcg
     9841 cataaaggtc gtgcacggta acattgctga gctgcgtggc cggtttaagc agtacccggt
     9901 ttgccaccga gtcctgagat tccggatggg catacagcag caaaactttc gctggctgag
     9961 acatcatccc cctcccggta tggtttttgt gtatagtcgc tgttttgggc taccattgcg
    10021 cccggtgcgg cagctcgccc atacattaca ttatcataat gataagttaa catagtctga
    10081 acatacggcg ccttatgatt gttttctcct cgttacaaat tcgtcgcggc gtgcgcgtcc
    10141 tgctggataa tgccaccgcc accatcaacc ccgggcagaa agtcggcctg gtgggtaaaa
    10201 acggctgtgg taaatctacc ctgctggcat tgctgaaaaa tgaaatcagc gccgacggcg
    10261 gcagctacac ctttccggga agctggcaac tggcgtgggt gaatcaggaa acaccagcgt
    10321 taccgcaagc ggcgctggaa tatgtcattg acggcgaccg tgaatatcgt caactggaag
    10381 cgcagctata cgacgccaac gaacgtaacg acggtcacgc cattgcgacc attcatggca
    10441 agctggatgc tatcgacgca tggagtattc gctcccgcgc cgccagcctg ctgcacggcc
    10501 tcggtttcag caacgaacaa ctggagcgtc cggtaagtga tttctccggt ggctggcgta
    10561 tgcgccttaa ccttgctcag gcgctgattt gccgttcaga cttgctgctg ctcgacgaac
    10621 cgactaacca cctcgatctc gatgccgtta tctggctgga aaaatggctg aagagctatc
    10681 agggcacgct gatcctgatc tctcacgacc gcgacttcct cgatccgatc gtcgataaaa
    10741 tcattcatat cgaacaacaa agcatgttcg agtacaccgg taactacagc tcatttgaag
    10801 tgcagcgcgc cacccgtctg gcgcagcaac aggcgatgta tgaaagccag caggaacgcg
    10861 tggcgcatct gcaaagttat atcgaccgtt tccgtgccaa agccaccaaa gcgaagcagg
    10921 cccagagccg cattaagatg ctcgagcgta tggagctgat tgcccccgca cacgtcgaca
    10981 acccgttccg ctttagcttc cgcgcgccgg aaagcctgcc aaatccgtta ctgaagatgg
    11041 aaaaagtcag tgcgggctat ggtgatcgca ttattctcga ctcgattaaa ctgaatctgg
    11101 tccccggctc gcgcattggt ctgttaggcc gcaacggcgc gggtaaatcg acattaatca
    11161 aactgttagc cggcgaactt gcgccagtca gcggtgaaat tggtctggca aaagggatca
    11221 agctcggtta cttcgcccag catcaactgg aatacctgcg cgccgacgaa tcgcctattc
    11281 aacatctggc acgtttagcg ccgcaggagc tggagcaaaa actgcgtgac tacctcggcg
    11341 gctttggttt ccagggcgat aaagtaaccg aagaaacacg ccgcttctcc ggtggggaaa
    11401 aagcccgcct ggtgctggca ttaattgtct ggcagcgtcc gaatctgctg ctgctcgacg
    11461 aaccgaccaa ccaccttgac ctcgacatgc gtcaggcact caccgaagca ttaatcgagt
    11521 tcgaaggtgc gctggttgtc gtctcgcacg accgtcattt gctgcgttcc accactgacg
    11581 atctctacct ggttcacgat cgtaaagtcg aaccgttcga cggcgatctg gaagattatc
    11641 aacagtggtt gagcgacgta caaaagcagg aaaaccagac cgacgaagcg ccaaaagaga
    11701 acgcgaacag cgcccaggca cgtaaagatc agaagcgtcg ggaagcggag ctgcgtgcgc
    11761 aaacccagcc actgcgtaaa gagattgccc gtctggaaaa agagatggag aagctgaacg
    11821 cgcaactggc gcaggcggaa gagaaactcg gcgacagcga actgtatgat cagagccgta
    11881 aagcggagtt gaccgcctgc ctgcaacagc aagccagcgc caaatccggc ctggaagagt
    11941 gcgaaatggc gtggctggaa gcccaggagc agcttgagca gatgctgctg gaaggccaaa
    12001 gcaactgatg gcgcagataa cgacgaccga tgccaatgaa ttcagcagca gtgctgaatt
    12061 cacccctatg cgcggcttta gcaattgtca tctgcaaacc atgctgccgc gtctgtttcg
    12121 tcgcaaggtg aaattcaccc cgtactggca gcggctggag ttgcccgacg gcgattttgt
    12181 cgatctcgca tggagtgaag accctgccca ggcgaaccat aaaccgcgtt tagtggtgtt
    12241 tcacgggctg gagggcagcc tcaatagccc ttacgcccac ggtctggtcg aggcggcgca
    12301 aaaacgcggc tggctgggcg tggtgatgca ttttcgcgga tgcagcggtg aaccaaaccg
    12361 tatgcaccgc atttaccatt cgggcgaaac cgaagacgcc agctggtttt tacgctggct
    12421 gcagcgcgaa tttggacatg cgccaacggc tgccgtcggc tattcgctcg gcggtaatat
    12481 gctcgcctgt ttgctggcaa aagaaggtaa taacctcccg attgatgcag cggtaattgt
    12541 ctccgcgcca tttatgctgg aagcctgtag ttatcatatg gaaaagggct tttcccgcgt
    12601 ttatcagcgt tacttgctga acctgttaaa agccaatgct gcgcgcaaac tggcagccta
    12661 tcccggtacg ctgccgatta atctcgcaca gttaaaatcg gtacgtcgca tccgtgaatt
    12721 tgacgatctg atcaccgcca gaattcacgg ctacgctgac gctatcgact attatcgtca
    12781 gtgtagcgcc atgccgatgc tgaaccagat cgccaaaccg acgctgatta ttcacgccaa
    12841 agacgatccg tttatggatc atcaggtgat cccgaaaccg gaaagtctcc ccccgcaggt
    12901 ggagtatcaa ctgactgaac atggcggtca tgttggcttt attggcggta cgttacttca
    12961 tccgcaaatg tggctggagt cacgcattcc tgactggtta acaacgtatc tggaggcgaa
    13021 atcatgttga ttccgtggca agacctctcc cccgaaacgc tggaaaattt aattgaaagc
    13081 tttgtgttac gtgaaggcac cgattatggt gaacatgagc gtacacttga acagaaagtc
    13141 gccgacgtca aacgccagct acagtgcgga gaagcggtgc tggtatggtc ggagctgcac
    13201 gaaacggtca atatcatgcc gcgcagtcag tttcgcgaat aacctgcaac cgtcggcgga
    13261 cgagataata atgatagcta agtcatggag ttactatgtc tgccaaacat ccggtcattg
    13321 cggtaacagg atccagcggc gcggggacca ccaccaccag cctcgcgttt cgtaaaatat
    13381 tcgcgcagtt aaatctgcat gcagccgagg tggaaggtga cagttttcac cgttacaccc
    13441 gcccggaaat ggacatggcg atccgcaaag cgcgcgacgc cgggcggcat atcagctact
    13501 tcggccccga agcgaacgac ttcggcctgc tggaacaaac cttcattgaa tacggtcaga
    13561 gcggcaaagg gaaatctcgc aaatatctgc atacctacga cgaagccgta ccgtggaatc
    13621 aggtaccggg gacattcacc ccatggcaac ctttaccgga acccactgat gtactgtttt
    13681 atgaaggttt acacggcggc gtcgtcacgc cacagcataa cgttgcgcag catgtggatt
    13741 tactggtcgg cgtggtgcct atcgttaacc ttgagtggat tcaaaaattg atccgcgaca
    13801 ccagcgagcg cgggcactca cgagaagcag tgatggactc agtagtgcgc tcaatggaag
    13861 actatatcaa ctacatcaca ccgcagtttt cccgcaccca tcttaacttc cagcgcgttc
    13921 ccaccgtcga cacctcaaac ccgttcgcgg caaaaggtat cccgtcgctg gatgaaagct
    13981 ttgtggtgat ccattttcgt aatctggaag ggatcgattt cccctggctg ctggcgatgt
    14041 tgcagggctc attcatttcc cacatcaata cgttagtggt gcccggcggc aaaatgggtc
    14101 tggcaatgga attaattatg ctgccgctgg tgcaacgatt gatggaagga aagaaaatcg
    14161 agtaactctg ctattacgcc ggataaaata ctatccggct tcacaacggg atagttaaat
    14221 cacgcggcaa ccacttcata cgagtgagta atattcaccg ctttttccag catcaacgcc
    14281 actgaacaat atttctcagc agagagatca accgcacgcg caaccgctgc gtctttcagg
    14341 tcgcgaccgg tgacgataaa atgcagatta atgtgcgtaa acaggcgtgg tgcctcttcg
    14401 cggcgttcag aggtcaattt tacttcacaa tcgaccacat cctgacgccc tttttgcagg
    14461 atagaaacca catcgatggc actgcaaccc cccgccgcca tcaacaccat ttccatcgga
    14521 cttggtgctt tatcgcctga gttgccgtcc attaaaatct ggtgaccaga ggcggattcg
    14581 cccagaaaag ttaacccttc gacccacttc actcgcgctt gcatttttgc tactccactg
    14641 cgtcaatttt cctgacagag tacgcgtact aaccaaatcg cgcaacggaa ggcgacctgg
    14701 gtcatgctga agcgagacac caggagacac aaagcgaaag ctatgctaaa acagtcagga
    14761 tgctacagta atacattgat gtactgcatg tatgcaaagg acgtcacatt accgtgcagt
    14821 acagttgata gccccttccc aggtagcggg aagcatattt cggcaatcca gagacagcgg
    14881 cgttatctgg ctctggagaa agcttataac agaggataac cgcgcatggt gcttggcaaa
    14941 ccgcaaacag acccgactct cgaatggttc ttgtctcatt gccacattca taagtaccca
    15001 tccaagagca cgcttattca ccagggtgaa aaagcggaaa cgctgtacta catcgttaaa
    15061 ggctctgtgg cagtgctgat caaagacgaa gagggtaaag aaatgatcct ctcctatctg
    15121 aatcagggtg attttattgg cgaactgggc ctgtttgaag agggccagga acgtagcgca
    15181 tgggtacgtg cgaaaaccgc ctgtgaagtg gctgaaattt cgtacaaaaa atttcgccaa
    15241 ttgattcagg taaacccgga cattctgatg cgtctgtctg cacagatggc gcgtcgtctg
    15301 caagtcactt cagagaaagt gggcaacctg gcgttcctcg acgtgacggg ccgcattgca
    15361 cagactctgc tgaatctggc aaaacaaccg gatgctatga ctcacccgga cggtatgcaa
    15421 atcaaaatta cccgtcagga aatcggtcag attgtcggct gttctcgtga aaccgtggga
    15481 cgcattctga agatgctgga agatcagaac ctgatctccg cacacggtaa aaccatcgtc
    15541 gtttacggca ctcgttaatc ccgtcggagt ggcgcgttac ctggtagcgc gccattttgt
    15601 ttcccccgat gtggcgcaga ctgatttatc accccgatat taactatgca ctgcgacaaa
    15661 cgctggtgct atgtttgccc gtggccgttg ggttaatgct tggcgaatta cgattcggtc
    15721 tgctcttctc cctcgttcct gcctgttgca atattgcggg ccttgatacg cctcataaac
    15781 gttttttcaa acgcttaatc attggtgcgt cgctgtttgc cacctgtagc ttgctgacac
    15841 aggtgctact ggcaaaagat gttcccctgc cctttttgct gaccggatta acgctggtac
    15901 ttggcgtcac tgctgagctg gggccattgc acgcaaaatt gcttcccgca tcgctgctcg
    15961 ccgccatttt taccctcagt ctggcgggat acatgccggt ctgggaaccg ttgctcatct
    16021 atgcgctggg cactttgtgg tacggattgt ttaactggtt ttggttctgg atctggcgcg
    16081 aacaaccgct acgcgagtca ctaagtctgc tgtaccgtga actggcagat tactgtgaag
    16141 ccaaatacag cctgcttacc cagcacaccg accctgaaaa agcgctgccg ccgctgctgg
    16201 tgcgccagca aaaagcggtc gatttaatta cccagtgcta tcagcaaatg catatgcttt
    16261 ccgcgcaaaa taataccgat tacaagcgga tgctgcgtat tttccaggag gcgctggatt
    16321 tgcaggaaca tatttcggtc agtttgcatc agccggaaga ggtgcaaaag ctggtcgagc
    16381 gtagccatgc ggaagaagtt atccgctgga atgcgcaaac cgtcgccgct cgcctgcgcg
    16441 tgctggctga tgacattctt taccatcgcc tgccgacgcg ttttacgatg gaaaagcaaa
    16501 ttggcgcact ggaaaaaatc gcccgccaac acccggataa tccggttggg caattctgct
    16561 actggcattt cagccgcatt gcccgcgtgc tgcgcaccca aaaaccgctc tatgcccgtg
    16621 acttactggc cgataaacag cggcgaatgc cgttacttcc ggcgctgaaa agttatttgt
    16681 cactaaagtc tccggcgcta cgcaatgccg gacgactcag tgtgatgtta agcgttgcca
    16741 gcctgatggg caccgcgctg catctgccga agtcgtactg gatcctgatg acggtattgc
    16801 tggtgacgca aaatggctat ggcgcaaccc gtctgaggat tgtgaatcgc tccgtgggaa
    16861 ccgtggtcgg gttaatcatt gcgggcgtgg cgctgcactt taaaattccc gaaggttaca
    16921 ccctgacctt gatgctgatt accacccttg ccagctacct gatattgcgc aaaaactacg
    16981 gctgggcgac ggtcggtttt accattaccg cagtgtatac cctgcaactg ttgtggctga
    17041 acggtgagca gtacatcctt ccgcgtctta tcgataccat tattggttgt ttaattgctt
    17101 tcggcggtac tgtctggctg tggccgcagt ggcagagcgg gttattgcgt aagaacgccc
    17161 atgacgcttt agaagcctat caggacgcga ttcgcttgat tctgagcgag gacccgcaac
    17221 caacgccact ggcctggcag cgaatgcggg tgaatcaggc gcataacact ctgtataact
    17281 cattgaatca ggcgatgcag gaaccggcat tcaacagcca ttatctggca gatatgaaac
    17341 tgtgggtaac gcacagtcag tttatagttg aacatatcaa tgccatgacc acgctggcac
    17401 gggaacaccg ggcattgcca cctgaactgg cgcaagaata tttacagtct tgtgaaatcg
    17461 ccattcagcg ttgtcagcag cgactggagt atgacgaacc gggtagttct ggcgatgcca
    17521 atatcatgga tgcgccggag atgcagccgc acgaaggcgc ggcaggtacg ctggagcagc
    17581 atttacagcg ggttattggt catctgaaca ccatgcacac catttcgtcg atggcatggc
    17641 gtcagcgacc gcatcacggg atttggctga gtcgcaagtt gcgggattcg aaggcgtaat
    17701 gcaggctaaa ccatcggata cagcgttcac gccgcatccg acatcttttg cctgatgcgt
    17761 cttatttacc cccaatcacc ttcgccaccg cgtgggcgaa acgatgcatc ccttcatcga
    17821 tatccgcatc ttccaccacc agcgacggcg caaaacgcat cacatcaggc ccggcattca
    17881 gcaccattac gccttcctct gcacccgcat acaggaaatc acgcgcctga cctttgtact
    17941 gtggtttcag ctctgcgcca atcaatagcc ccataccgcg aatatcgcta aacacatcgt
    18001 actgctgatc gatcttctgc aaatggtcaa caaaacgctg acgtttcgcc tgaatgcctt
    18061 ccagcacttc aggggtattg atgatatcaa acgccgcccc cgctactgca caggccagag
    18121 gattaccgcc gtaggtggaa ccgtgagaac caggatgaaa tgcagaagca atttccgccg
    18181 tggtcagcat ggcgctaatc gggaagccgc cgcctaacgc tttcgcagag gtcagaatat
    18241 ccggcgtcac gccgtagtgc atgtaagcaa acaaatcgcc ggtccgcccc atcccgcact
    18301 gcacttcatc aaataccaat aatgcctgat gttgatcgca cagctcgcgc aagccctgca
    18361 aaaactctgg cgtcgctgcc gtcacgccgc cctcgccctg gatcggctca accaccaccg
    18421 cacaggtgtg atcatccatc accgctttca ctgcgtggag atcgttaaag ggaacgtgga
    18481 tgatgtctgc cggttttggc ccaaagccat cggaatattt tggttgcccg cccaccgaga
    18541 cggtaaacag cgagcgacca tgaaaagcgt tatggaaggc gataattttg gttttgaacg
    18601 ggctatgacg cacacaggcg taatggcgtg ccagtttaaa ggcagtttcg ttagcttccg
    18661 tgccggagtt cataaacacc acgcgttcgg caaacgttgc ctcaatcagt ttacggccaa
    18721 gacgcagcgc cggctcattg gtgaaaacgt tactgatatg ccacagagtt tcgccctggg
    18781 tttttaacgc gttcaccagc gcaggatggc aatggcccaa cgccgtaact gcaatgccac
    18841 ccgcgaaatc gacatactcc ttgccttgct gatcccagat tcggctgccc tgacctttta
    18901 ccggaataaa ctctgccgga gcataaatcg gcaggatcac ttcatcgaaa gtcgcgcgtg
    18961 taattgctgt ttgttcaatt gccatctcat gatcaccctc ttacgcataa atcaatgtga
    19021 aattataatc acaaaatatg cataaaaaat cacttaatag caatcagaaa tcagcgatgc
    19081 aggaaattag ccagcagttg atgtccttgt tcgctaagaa tactttctgg atggaactgc
    19141 acaccttcca gatcccactg gcgatggcga atccccataa tctcgcgggt ttcgctccag
    19201 gccgtcacgt caaagcacgc tggtaatgag tcaggttcca ccaccagcga atggtagcgt
    19261 gtcacggtaa gtggatttgc cagcccccga aatacgccct caccgttatg tgttatcggc
    19321 gaggttttgc cgtgcatgac ctttgcggtg cgcacaactt taccgccaaa tgcctgcgcc
    19381 attgcctgat gaccgaggca gacgccaaga atcggcaagc gcccggcata gtggcgaata
    19441 acgtcaaggg agatcccggc ttcatctggc gtacaggggc cgggtgagat gacaattttt
    19501 tgtggtttaa gggcgtcgat atccgccagc gtcaacgcat cgttgcgctt aaccagcaca
    19561 tccgccccca gttcacaaaa gtactggtag aggttccagg taaaagaatc gtagttatct
    19621 ataagcagga tcatggcggc tccggtacga aagaaccgcg ctattttact cagattcccc
    19681 ggcttcgctt accactttgc taaatatcgt ctgcagcgcg gtgagatccc ccattgcccc
    19741 actctgattt gcctgattcc aggcctcttt ttcgataccc tgccagctca gttgatatcc
    19801 ggcatgaatc gccagttgct cgaagaagat ccgctgtgcc agaccacttc ccacccggaa
    19861 gggatgcagc acgttgattt cacaatagta atgcgccagc cgctcgacga acttcgcttt
    19921 ctccaggcca accagataac cttcttcctc cagatcctgc atcagggcat tgccctcttt
    19981 ttcgatataa gcaaagtggc agaacggcgt atcgccctga taaatatcaa cttcacgcag
    20041 ttgccccgcc cagtcgaaaa tatcctgata cagctggcga tggatagtgc gcaaatgcgg
    20101 taaaccgcgc accagcggac caagctcaat ggtcgcagca cgcagcgccg tcatttcgta
    20161 agcggcctgt tccagccgct gctgctggtg gatgttcagc cggttacgca tgatatcaag
    20221 gccaggataa agatacggat cgcgcccttc gccgaattta tcgctcatag tgcctcctca
    20281 gcgcttcaag gcgcgctaaa gcctctgtgg cagtaagagt gactaaaggc atctcgacgc
    20341 cttcaagacg gcgactggcc tggaaattac gattacgctg aagctcccag agacgggact
    20401 tttgcttatc ggtgagtttc ttcacttgat gcctccctga acgtgtccat ttgccacaag
    20461 tataagcggc aaatccgggt tacgccggga gagcagaagc gcgggcaaga ttgcccgcgc
    20521 gagaaatcat tacggcagca ctttagcgga aaggataact accggttttg acggcacatt
    20581 ctggtacgga ccaacgtcat gagtcggcac ctgggaaatc ttatcggcaa cgtccatgcc
    20641 tttcaccact ttaccaaata ccgcgtaacc gaaatcacgc tgaccgtggt caaggaaggc
    20701 gttatcggca acgttgataa agaactggct ggtggcgctg tctttgtcag cggtacgcgc
    20761 catcgcgatg gtgccacgcg tgttgcgcag gccgttatct gcttcatttt tgattggcgg
    20821 gtttggtttt ttctgctgca tctgctcggt gaaaccgccg ccctgaatca taaagccagg
    20881 aatgacgcgg tgaaaggtcg tgttgttata aaaaccactg ttcacataat cgacaaagtt
    20941 ttgcacagac actggcgctt tttgtttatc cagctccagt tcgatgttac cagctgaggt
    21001 tgtcaacaat acgtgcgggt cccctttcgc tgccattgct gcgggagaaa gagcagaaag
    21061 agcgaaaaca gcagccatcg ccgccagggt cgatttgaac atgagatttc cttaacaaac
    21121 agagcagaaa aaaagcaagt gaagagattc taaatagcct tcaagaacaa ggccatccct
    21181 ttacctaatt ttacgtatct gaaatatatg taacctgacg atcacttttt attctgtgat
    21241 caaaatcacc tcttaaaatg caatttagca accgattgca ataaaacatt taaacagatc
    21301 acaaaatcac ctaaaatcgc ccgctcgcgg catatcccgc tatgccattt tatttttgaa
    21361 cgcaggccaa tcatgactaa cagcaatcgc atcaagctca catggattag ctttctctcc
    21421 tacgcactga ccggtgcgtt ggttattgtc accgggatgg tgatgggaaa tatcgccgat
    21481 tatttcaatc tgcctgtttc cagtatgagt aataccttca ccttcctcaa cgccggcatt
    21541 ttaatctcta tcttcctcaa cgcctggctg atggaaatcg tcccgttgaa aacgcagtta
    21601 cgttttggct ttctcctgat ggtgctggcg gttgccggtt tgatgttcag ccacagcctg
    21661 gcactgttct cggcggcgat gttcattctc ggggtggtca gcggcatcac catgtcgatt
    21721 ggtacattcc tggtaacaca aatgtatgaa ggacgtcagc gcggttcacg cctgttattt
    21781 accgactcct tcttcagtat ggccgggatg attttcccaa tgatcgccgc gtttctgctg
    21841 gcgcgcagca ttgagtggta ctgggtttat gcctgcatcg ggctggtgta cgtcgctatc
    21901 tttattctga ccttcggctg tgagttcccg gcgctgggta aacatgcgcc aaaaacggac
    21961 gctccggtag cgaaagaaaa atgggggatc ggcgtgctgt ttctctccat tgcggcactg
    22021 tgctacatcc tcggtcagtt aggttttatc tcctgggtgc ctgagtatgc caaaggcctg
    22081 ggcatgagcc tgaacgacgc gggcacgctg gtgagtaact tctggatgtc atacatggtc
    22141 ggcatgtggg cgttcagctt tattcttcgc ttctttgatt tgcaacgcat tctgaccgta
    22201 ctggctggtc tggctgcgat tctgatgtac gtctttaaca ccggaacacc ggcacatatg
    22261 gcgtggtcaa ttctcgccct gggcttcttc tccagcgcga tctataccac catcatcact
    22321 ttgggttcac agcagaccaa agtaccgtcg ccaaaactgg ttaactttgt cctgacctgc
    22381 gggaccatcg gtactatgtt gacctttgtg gttaccggcc cgattgttga acatagcggt
    22441 ccgcaggcgg cgctgctgac ggcaaacggt ctgtacgctg tcgtctttgt gatgtgcttc
    22501 ctgttaggtt tcgtcagccg tcaccgtcag cataacaccc tgacctctca ttaattgctc
    22561 atgccgggcg gcactatcgt cgtccggcct tttcctctct taccgcgcta cgtacatcta
    22621 tttctataaa cccgctcaat ttgtctgttt tttgtacaaa catgaaatgt cagacaattc
    22681 cgtgacttaa gaaaatttat acaaatcagc aatatacccc ttaaggagta tataaaggtg
    22741 aatttgattt acatcaataa gcggggttgc tgaatcgtta aggtaggcgg taatagaaaa
    22801 gaaatcgagg caaaaatgag caaagtcaga ctcgcaatta tcggtaacgg tatggtcggc
    22861 catcgcttta tcgaagatct tcttgataaa tctgatgcgg ccaactttga tattaccgtt
    22921 ttctgtgaag aaccgcgcat cgcttatgac cgcgtacacc tctcgtctta cttctctcac
    22981 cacaccgccg aagagctgtc gctggtgcgc gaaggcttct acgagaaaca cggcatcaaa
    23041 gttctggtcg gtgaacgcgc tatcaccatc aaccgtcagg agaaggtgat tcactccagc
    23101 gccggacgta ccgtttttta tgacaagctg atcatggcaa ccggttccta cccgtggatc
    23161 ccgccaatca aaggttctga tactcaggac tgctttgtct atcgcaccat tgaagacctc
    23221 aacgccattg aatcctgtgc ccgtcgcagc aaacgcggtg ccgttgttgg tggcggcctg
    23281 ttaggtctgg aagccgctgg cgcgctgaaa aacttaggta ttgaaaccca cgttatcgaa
    23341 tttgccccta tgctgatggc ggaacagctt gatcagatgg gtggcgagca gctgcgtcgc
    23401 aaaatcgaaa gtatgggcgt gcgcgttcac accagcaaaa acacccttga gattgtgcag
    23461 gaaggtgtcg aagcgcgtaa aaccatgcgt tttgccgacg gtagcgagct ggaagtcgac
    23521 tttatcgtct tctcaaccgg tatccgtccg cgcgataagc tggcaaccca gtgtggtctg
    23581 gacgttgctc cgcgtggggg tattgtcatc aatgattcct gccagacttc cgatccggat
    23641 atctacgcca tcggtgaatg cgcaagctgg aacaaccgtg tatttggtct ggtagcacca
    23701 ggctacaaaa tggcgcaggt cgccgttgac catattcttg gtagcgaaaa cgcctttgaa
    23761 ggtgctgacc ttagcgctaa gctgaaactg ctgggcgtag acgtaggcgg tattggtgat
    23821 gcgcatggtc gcacgcctgg cgcacgtagc tacgtttacc tcgacgaaag caaagagatc
    23881 tacaaacgcc tgattgtcag cgaagacaac aaaaccctgc tcggtgcggt actggtgggc
    23941 gataccagcg actacggcaa cctgctgcaa ctggtgctga acgctatcga actgccggaa
    24001 aacccggatt ctctgattct gccggcgcac tcaggtagcg gtaagccgtc tatcggtgtt
    24061 gataaactgc cggacagcgc gcaaatctgc tcctgcttcg acgtcaccaa aggcgatctg
    24121 attgcagcta tcaacaaagg ctgccacacc gttgcggcgc tgaaagctga aaccaaagcg
    24181 ggtactggct gcggtggctg tatcccgctg gtcactcagg tactgaacgc ggaactggcg
    24241 aaacagggca tcgaagttaa caacaacctg tgcgaacact ttgcttattc gcgtcaggaa
    24301 ctgttccatt tgatccgcgt tgaaggcatt aaaaccttcg aagaactgct ggcgaaacac
    24361 ggcaaaggct acggttgtga agtttgtaaa ccgaccgtcg gttcgctgct ggcctcctgc
    24421 tggaacgaat acattctgaa gccggaacat actccgctgc aggattcaaa cgacaacttc
    24481 ctcgctaaca tccagaaaga cggcacctac tcggtgatcc cgcgttctcc gggcggtgaa
    24541 atcaccccgg aagggctgat ggcagtaggt cgtatcgcgc gtgaatttaa tctctacacc
    24601 aaaatcaccg gctctcagcg cctggcgatg tttggcgcac agaaagacga tctgccggaa
    24661 atctggcgtc agctgattga agccggcttc gaaaccggtc atgcctatgc gaaagcactg
    24721 cgtatggcga aaacctgcgt gggtagcacc tggtgccgct acggcgttgg cgacagcgtc
    24781 ggcctcggcg tggaactgga aaaccgctac aaaggcatcc gtacgccgca caaaatgaag
    24841 ttcggtgtct ctggctgtac ccgtgaatgt tcagaagctc agggtaaaga cgtgggtatc
    24901 atcgccaccg aaaaaggctg gaacctgtat gtttgcggta acggcggcat gaaaccgcgt
    24961 catgcggatc tgctggcagc ggatatcgat cgcgaaacgc tgattaaata tcttgaccgc
    25021 ttcatgatgt tctacatccg tactgccgac aaactgacgc gtaccgcgcc gtggttagaa
    25081 aacctcgaag gtggcatcga ttacctgaaa gcggtgatca ttgacgacaa actggggctg
    25141 aacgcacatc tggaagaaga gatggcgcgc ctgcgtgaag cggtagtgtg tgagtggact
    25201 gaaacggtca atacaccgtc cgcgcagact cgcttcaaac acttcatcaa cagcgacaag
    25261 cgtgacccga acgtgcagat ggtgccagag cgcgaacagc accgtccggc aacgccgtat
    25321 gaacgtatcc cggtaactct ggtggaggac aacgcatgag ccagtggaaa gacatctgca
    25381 aaatcgatga catcctgcct gaaaccggcg tctgcgcgct gttaggtgac gagcaggtag
    25441 caattttccg cccgtatcac agcgaccagg tgtttgcgat cagcaacatc gacccgttct
    25501 tcgagtccag cgtgctgtca cgcgggctga ttgcggaaca ccagggcgag ctgtgggtcg
    25561 ccagcccgct gaaaaaacag cgttttcgct taagcgacgg cttgtgcatg gaagacgaac
    25621 agttttccgt caagcattac gacgcgcgag tgaaagacgg cgtggtgcag ctacgcggtt
    25681 aatgttttaa cgggaggcac aatgcctccc ctttttgcat ggtcctgtaa taaccttggt
    25741 atattgcagg acatttttta aactttttgt tttatttttt gtttttattt tttaaaggat
    25801 aatcaaatgt ttacagacac tattaataag tgtgcggcta acgctgcgcg cattgcacgc
    25861 ctgtcggcaa ataacccgct cggcttctgg gtcagctccg caatggcggg ggcgtatgtg
    25921 ggtcttggga tcatcctgat tttcacgctc ggtaatttgc tcgacccgtc cgtacgtcct
    25981 ctggtgatgg gcgcgacctt tggtatcgcc ttaacgctgg tcattattgc tggttctgaa
    26041 ctgttcaccg gacacaccat gttcctcacc tttggagtga aagcgggcac tatcagccac
    26101 gggcaaatgt gggcaatcct gccgcaaacc tggctgggta acctggttgg ttccgtcttt
    26161 gttgccatgc tctatagctg gggcggcggt agcctgctgc cggtagatac cagcatcgtt
    26221 cactccgtcg cgctggctaa aaccactgca ccggcaatgg tactgttctt caaaggtgca
    26281 ttgtgtaact ggctggtttg cctggctatc tggatggcgc tgcgcactga aggggcggcg
    26341 aaatttatcg ctatctggtg gtgtctgctg gcatttatcg cgtccggcta cgagcactcc
    26401 atcgcgaaca tgacgctgtt cgccctctcc tggtttggca accacagcga agcctacacg
    26461 ctggcgggta ttggtcataa cctgctgtgg gtgacgctgg gtaatacttt atcaggtgcc
    26521 gtattcatgg gattgggtta ttggtatgct acgccgaaag cgaatcgtcc ggttgcggac
    26581 aaatttaatc aaactgaaac agctgccggt taattactaa ggggttttta cgtggatcat
    26641 ttgcctatat tttgccaatt acgcgatcgc gactgtctga ttgtcggcgg tggtgatgtc
    26701 gcggaacgca aagcaaggtt gctgttagac gcaggcgctc gcttaacggt gaatgcatta
    26761 gcgtttattc cacagttcac cgcatgggca gatgcaggca tgttaaccct cgtcgaaggg
    26821 ccatttgatg aaagccttct cgacacctgc tggctggcga ttgcagcgac agatgatgac
    26881 gcgcttaacc agcgcgtcag tgaagccgct gaagctcgtc gcatcttctg taacgtggtc
    26941 gatgcgccga aagccgccag ctttattatg ccgtcaatta ttgaccgctc accgctcatg
    27001 gtcgcggtct cctctggcgg cacctctccg gttctggcac gcctgttgcg tgaaaaactt
    27061 gaatcactgc tgccgttgca tctgggccag gtagcgaaat acgccgggca attacgcggt
    27121 cgagtgaaac aacagttcgc cacaatgggt gaacgtcgcc gtttctggga aaaactgttc
    27181 gttaatgacc gtctggcgca gtcgctggca aacaacgatc agaaagccat tactgaaacg
    27241 accgaacaat taatcaacga accgctcgac catcgcggtg aagtggtgct ggttggcgca
    27301 ggtccgggcg atgccgggct gctgacactg aaaggactgc aacaaattca gcaggcagat
    27361 gtggtggtct acgaccgtct ggtttctgac gatattatga atctggtacg ccgcgatgct
    27421 gatcgcgttt tcgtcggcaa acgcgcggga taccactgcg taccgcagga agagattaac
    27481 cagatcctgc tgcgggaagc gcaaaaaggc aaacgcgtgg tgcgactgaa aggcggcgat
    27541 ccgtttattt ttggccgtgg tggcgaagag ctggaaacac tgtgcaatgc aggcattccg
    27601 ttctcggtgg ttccgggtat taccgcagct tctggttgct ctgcctattc gggtattccg
    27661 ctcacgcatc gcgattatgc ccagagcgta cgcttaatta ccggacactt aaaaaccggt
    27721 ggcgaactgg actgggaaaa cctggcggca gaaaaacaga cgctggtgtt ctatatgggg
    27781 ttgaatcagg ccgcgactat tcagcaaaag ctgattgaac acggtatgcc tggcgaaatg
    27841 ccggtggcaa ttgtcgaaaa cggaacggca gtcacgcagc gcgtgattga cggtacgctc
    27901 acgcagctgg gcgaactggc gcagcaaatg aacagtccat cgctaattat tattggtcgg
    27961 gttgttggcc tgcgcgataa attgaactgg ttctctaacc attaatttaa ccagggccag
    28021 agaattctgg cctttttcgt ttacaactct ctttatatat tcagcagata aaaatacccc
    28081 ccttaataaa tattatctgc taatgggtta tattcaccgt tacagcgact tacaaaatta
    28141 agtcattcgt tcactgacgc caattaaata ttcctgtatc attcttaatg agcagtctgc
    28201 ttatattcat ataatcaatg aatattaatc aatatataat acatagggaa tgtaaatgaa
    28261 caaatttatt aaagttgcac tggtaggtgc ggtactggct acgttaactg catgtactgg
    28321 tcatattgaa aaccgtgata agaactgctc ttacgactac ctgctgcacc cagctatttc
    28381 tatttctaaa atcattggcg gttgcggtcc tactgcacag taagtctctg acagaaattg
    28441 gcagatgctg atttaattgc ctgacgccct cgagattttc cgagggcgta ttttttctcc
    28501 atactcttcg aaaaacatcg ttcccagaat taaacgtcgg caatactctc gcgcaaaatc
    28561 cgcaataccg tctcttcacc gctgcgattt gggttaatgc gaatcgcgca atgttctaac
    28621 tgcggattcg cctggcgaaa cgttccggaa agtcgataaa agagcggcgg gatttcatat
    28681 ttcgactctg caccaaccgg gtaaggcaag gcaccgagtt tctgcgcctc ttccagcact
    28741 ctggcggcaa tcggctgatg aaactccaca atcaatacct tcgactgtgc attggcaatg
    28801 acggcgcttt tcacttccgc caccgcgccg ccgttaagca aagccagcaa ccgttcagat
    28861 acccccgcct gcaccgcgtg catcactggc gcgagcacca gaccacgcaa gacttccagc
    28921 gcctgcgcgc cctgaacctg gctgccgccg gaatagagcg tggcgcgaat ccggctaata
    28981 acatcggcat cgcctaccac cgcgccaaca ccctctggcc caaatagctt gaagcaggaa
    29041 aatgtcgaga cattcgcccc gcattcacag ccaatgcgcg ccaccttcat caccgcatag
    29101 ttatcatcgg ttaaggctgg aacacctgcc gagcgcaacg ttgccagcac atctgccaga
    29161 atgtagccgt cctgcggctg ctggcgcgta tgctgcacca gcgccgcatc cggttgttgc
    29221 tcgtcgacga cctgcttaag tgccgacagg tcattgaaat tagcagtaat aagcgtcagc
    29281 cccatctgct caataatgac ctgagtcgtc gggtaaacag gcgcgtcatg caccagaaga
    29341 cgctgccccg gtttgagcaa agccgccaac gccgcacgga tcgcgccagt acccgcgccc
    29401 tgtaccagcg ctgcagcctg tgcgtgaaat gcatcagcca gcacctgttc cactcgctgg
    29461 gtaatgcgcg gttgattcag ccccggcgtt aagcctaaat caccgccagc aagaaactcg
    29521 ctgccgggaa aatggcgaca aatgctatcc accagcgcaa actgcttttg ctgcgcctca
    29581 ataagcgtca ggctttgcag aggaaacgtc ttcataggtg ccttccttaa gccggaacga
    29641 acaggccaag ccagtaaaga acattcagca gaataccggt gatcattact gccacgaccg
    29701 gtgccgccat tttctgtacc ggacggccca gggattcgtt gaggaagtaa atggcaactg
    29761 cgatagagaa tccggtatag cccgccattt tgattgctgc aaagatcgaa ccgaccagca
    29821 acgccacttc catcagcata ttcatggcgt tacggatgtt atccgacgca ttacgcaccg
    29881 acgggtagcg ccccagccat ttgccgatcg aacgaagcag caggacttcc gccgaaataa
    29941 ccactgcgcc taataccgct gcaaccatcg gattcggcga gagatagccc accgcataaa
    30001 caaaggtaaa gcccgcaact gcatacacgc cggtggctag cgcggtggtg gcaatcatcg
    30061 gcacaaaacc cagcccgcgc ataaattccg ccagagctgc ctggttgatt agtgtttgcg
    30121 attgttccgg cgttacgcct gcggagtacg ctttctccag agtgaagatc gacacttcac
    30181 taccggcgaa aatcttcatg ctggcaacgg cggcaatcaa tgctcccacg atggcgatat
    30241 acggtaagtt tttgataatc cgtgacgtgc gttcttcaaa caccgaaagc ccgctggcat
    30301 caatgtcatt ttcatcacga tgacgcaggt cgtgagttat cgcgatcccc agcagcatca
    30361 ccataccaat aaagatttcg atggattcag ggttaagatg tgggaaatag cgcacgacga
    30421 ccacgcgggt catcagtacc accacggcgg cgatcagact ttgcttccag ccaaactggt
    30481 agaaaatcgc caccagcggg aagagtgcaa aagctgaaac caccggcgag cttaattccc
    30541 cgaggctacc taatacatcc accggtagcg cggtcagcag ctggtttacc ggcaacaggc
    30601 aagtaaggat caacacgccc cagatagcgc caagaccaaa cgccatcagg ctgtttatcg
    30661 ccagtacgcc gaggatatcg gtaggaagaa agagtaacca tgcgttgagc agcccggttt
    30721 tcagggtaaa agagatcccc accgaggcca caaaaccaat gctcagacca aaagcgatac
    30781 tccccgcctc gcgacggttc atatagcctt caatcagttg cggcaggatc gggcggatac
    30841 cgtcatgaaa aacggctgcc gagcgatgcg ccagaagcga tgtcataccc gtcaggcacg
    30901 ccaccacgat aatctgaata tacagatcca tagtcgtgcc ttttatttca gatggttaat
    30961 cagcatcgga atcgcgtgtt cgacgtgctc aacggaaagg ccaaacgcca cttttccttc
    31021 agcgatcatt ttggcgatat gctcgtcttt cgctttaatg cctggtttgg caatggtgca
    31081 acttttgtta tagccaatca ccgcgatggc aatcgacaat gccgcgcccg caccggtatt
    31141 acacgcgccg atgtagtaat ccagttgccc ggatttcact ttcatcgccg cttccatgtc
    31201 gttatgaatg aaaacttcaa aacagccagg agccgtcgct tcaatggttt ttttaatctg
    31261 ctcacgctgt aagcctgcaa caccaatctt tttcatgagt ctgtccttat tggaaaaatt
    31321 gagagggatt ttcacgtaac atcacatcca catcggcctg actgaatcct gactggcgca
    31381 attgcggaat aaaggtggtc aataaaaagt catagccata accaccgttg gcttttaaat
    31441 gagagcgccg cgtaatatcc atcgacagca tgacgcggtt cagcaaccca cggtcacgta
    31501 gcgcatggag catcgcaatc cgcttttcgt ccgggtagta actgttcttg ccgatggtgt
    31561 cgaactgcac atacgcgccg agatcgatca tcttcaaaat gttgtcgaga ttgtctttca
    31621 gatcgcagtg gccgacggtg acgcgcgaaa gatcaactcc gtgggcttgc aacagagcca
    31681 gttgctccag ccccatcgtg ctgaacgacg tatgcgtgga gatcgggcgt ccggtctggt
    31741 tatgcgccag cgcggcggca ataaacacct tctcttctag cgacgtaatc ttcccttcgc
    31801 tggagccgat ctccgcgata attccggctt tcaggtcagt gccatcgata ccctgctcaa
    31861 tttcatcgac catctcctgc gccagttcct gcacgctgcg ggtcgccaca tgttccggga
    31921 aaaacgcgtc ctggtaataa ccggtacagg ccaccacgtt gatcccggtt tcacgcatta
    31981 catcgagcat aaattgcgca ttgcgcccca tgtaacggtt ggtcatttca atcacattac
    32041 gcacgccccg ggccatcagg tcgttcatct cctggcaaat gaacgcatac tgatcaaggc
    32101 ggcagtccac gttgttttta aagccggaaa gatcaatatg cagatgctca tgggccaggg
    32161 tgtaacccgt cggatcaaaa ctcatagggt gtctcctgca aaccggaggg aggaaagaaa
    32221 agagcgacca ttttgtggcg gtggcgcacg gaagaattca cacacggtag cgcccacatc
    32281 ggagagcgtg gtgcgaacac caagctgcgt ggcagccagg ccttgctgat aaaccagcac
    32341 cggcacgact tcgcgggtat ggtggctgtg accgattgtc ggatcgttac cgtgatccgc
    32401 catcacgacc aggcaatcat ctggctgcat cgtctcaacc aggagagcaa gattacggtc
    32461 aacgacctgc aaacgttcag cataacgtgc gacgtcttct gcgtgaccag cgaggtcggt
    32521 ttcctgaatg ttggtgcaaa taaacgctgt ctgatgggtg ataaattcgt cgagggtgat
    32581 atccataatc cgctggctat ccaccaggtt ttgccagctc acgccataag gattgttgac
    32641 gatatctgcc accttaccca ccagcacggt tggcacgcct gcttcataca gtttttgtgg
    32701 tacctgcact ttttcatcga cgccatagcc catatgcacg acctggaaac cgttgtcata
    32761 agcgccagaa cgcggcgcgt tgatgccaat aaagcgccct tctttgcttt ctgcggcatc
    32821 gagaatgcgt tgactgtcgg ttaacaggcc gccaaatgta atgacccgcc cgaccggtac
    32881 ctgctcacgc acgatacgac caattttgat tgcgtcgtca aaagagacca cagagagatt
    32941 ggcggtgatg ttatagacct ggcctaaatc cgcctcgaga ttatcgccaa tcgcaaccgc
    33001 ctgattgacc cacagaaatt gcaactcttc gccacggcgc tccacctgcc aagcggcgga
    33061 aactaatgcc tgctcaacac ggtcaatcac atcgcgaaaa ggcatccgca gcggtggtaa
    33121 cgggcgcgtg cctaaaattt cctgatgccc cataaaggta tcgccacctt catgttgcag
    33181 ctctgccaca ccccaggttg cggagtctga cggctgcata tcacctggcg cataacccaa
    33241 tgcgttgatt agccccaact tctccagcgt tggtagctgc aaatgcggca gctggctcag
    33301 gatgtgacca caagtattcg ccccggcatc ctgtggacgc accagcgtga catctttcat
    33361 tgcgcctacg ccaaagctat caatcactaa caccacaaat cgcgccatta cgcccccaga
    33421 gagtttccaa gactgtcata acgaccgacg atttccggtt cgccacggtg aataccggac
    33481 agcaacacca catcgctacg ggtgacaaaa atctgcgtgc gaaaacagag cactactgca
    33541 ctgcttaccg gaaactcgcc tgccagcggc agggtgtagt cgatactgct gtcatccaca
    33601 gttttgagat tggtttcagt aatcttttga ttttctggcg taaacaccag cgcatgttgc
    33661 gcgtgaccac gacggtagta accgccgccg tagcagtagc tgtcgccacg gaaatgatgg
    33721 gagatttcac ttaaccagag catcgcgata cgttcaggct gatcgccctg ctggtttgcc
    33781 ggaatagtgc ccgtcagcgc atgaccgggt tcggcatgag tcacaccgta ttccgccagc
    33841 aatggcagcg aagtgcagct ggtcgctgaa ggcgcgttca gttgctcaat cgcaatacca
    33901 gatttcgcca gttgatcccg tgcttgtacc agcgtgtgaa gattcggtgt cggcaaaact
    33961 ttcccgacag cttcatccca aagcaggcaa gggaaatggg taagtcccgc caaatgtaac
    34021 cccggcagat tctggatttc ggcgacaatc tccggcaaca cctggagggc aaaaccgctc
    34081 tcctggcccg gataaagaaa atcatcgtcg ctataaactt tgagtagcac agactgcaca
    34141 cgtccggtct tcaacgctgc cgcagaaact tcccgcgctt tgtcgagagt aaacacggtg
    34201 atgacatcag ttccctgttc aacggcgtca gaaaccagat gacaagggat ttgtaccaga
    34261 tgcccctgat gcgccaccgg caaaccagcg cggcgcatga ctcgcgcctc tttgtaatcc
    34321 accgccacaa tgccgctgta gcctaatgcc agcaattttt ccgccagcca cggattacga
    34381 ccaaactgct tggtcatcag atacagttca atgccgtaaa gccgcgccgt ctcaatcagc
    34441 cgcttaccgt tttccagtat ctgatccacg tcgatcaccc agctgtccgg ggcgatcttg
    34501 cccttttgcc acaaacttag tgcggcggaa attagcgccg ggttctggcg tttcaatgct
    34561 tctacaaaca tcttgttgcc tcgtttctgt tccttgaatt aaatattcat ttttttgaat
    34621 atttaggttg tgcgacgtgg atttgtcggg agcttcaaac ctcttcgtta acagccatcc
    34681 ataatccata aaggttggcc agcaaatagc cttcttcaca cgggttaact tccagtgcaa
    34741 attccttcaa caacacctga tgcaattgca caacggcttg ccagtggctg gattgcgcca
    34801 gttctgccag caattcgtta tccagcggct ctatttcttc accgcggcga ctgcgcatca
    34861 gtgcgctcgc catatgtgtc atcgccatca ttccttgctc gctgcgcacc ggtagatgac
    34921 actctgtttc cagtacgttg acgacctgca tcatgccttt gcagatgtcc ttatcaataa
    34981 cgcctgcatc gcaaagcagg ttgagtctgg tttccatttc agatgccttt cgtttaataa
    35041 ctgggttcct gttccccact gaccactcgt tgttgatggg caagcagggc ctgtttatca
    35101 acaactgcac gcagcaattg ttgcatcggg taatcatcga ctcgcgtcaa aaccacggct
    35161 tcggtggcct gtaaaaatcg cggatcgtct gtgagcggcg tcgcttctaa tcccagcatg
    35221 gtcagttcgt tttccgccac cacgttccag atcaccgcat cgacatcgcc tttaacaatg
    35281 cgttgtaaac tctcgtgata agagagatcg acacgttcca catcactatc gccaaaaaag
    35341 acatcggtca tgattttctg atccgccgaa cggttatcca gccccacgcg cttcacgttt
    35401 gcggactcgc ctttacggca aatcaactgg tgctcgccaa cgtaggtgtg cggccccaac
    35461 tccagcgcga ggcataagcc tttttgcgtg agataacttt ccgccgccag tcgggaaaca
    35521 accgccatgt catacacgcc gttaagtaaa cactccacgc gaatatccgc gccgcgcata
    35581 tgcgcatagt aaaaaggaat accatcaaac tgagctttca atccgctcgc caggccttcg
    35641 tacaaacggg tatagggcaa gggcatcgcg cacaccacat tgttgatatc tacatgagtc
    35701 agcaatgctt tgttatccat ctcgaccaga taactgccat tgcgcccacg gcgttcaatc
    35761 cgtatcgctc cgcttgattc cagcgttttc aacgcggcct gcgtcaggcc aaccgatgaa
    35821 cggcattcag ttgccagctc atctatggtt ttcagtcgat taccgcactt ttcacccaac
    35881 aaataacggg ccagtgtcgt gataacgacg ccttcttttt tgataaacgt tcgacgcata
    35941 atatattttc aataaaatga atgtttatat cttcattaaa atgaagataa gtggcaactg
    36001 tgagatgtgg aaacggcgag gtgcttcaca ttttttcgga ttattgcaaa tgaggataaa
    36061 aaaaccgggt ttccccggtt tttggcatta atgttaaatt acggcttcgc cacaaaacca
    36121 atcgcttcgt acaccgcttt cagcgtacga gaagcgtgtg cactggcttt ttccgcgccg
    36181 tctttcatca cctgttgcag gaaggcttca tcgttgcgga aacggtgata acgttcctgc
    36241 aattcagtca gcataccgga aacggcatca gccacttcgc ctttgagatg accatacatc
    36301 ttgccttcga actgtttttc cagttccggg atgctctggc ccgttaccgc agaaaggata
    36361 tccagcaggt tggaaacgcc cgctttgttc tgcacatcgt agcgaactac cggcggctcg
    36421 tcggagtcag tgaccgcacg tttgattttc ttcactaccg atttcggatc ttccagcagg
    36481 ccgataacgt tattgcggtt atcgtcagac ttggacatct ttttggtcgg ctccagcagc
    36541 gacattacgc gcgcgccgga tttcggaata aacggctccg gcactttaaa gatatcgcca
    36601 tacagcgcgt tgaaacgctg ggcgatatcg cggctcagtt ccaggtgctg tttctggtct
    36661 tcacccaccg gtaccagatt agtttgatac agcaggatgt ccgcagccat cagcaccgga
    36721 tagtcaaaca gaccagcatt gatgttctcg gcataacgcg cagatttatc tttaaactgc
    36781 gtcatgcggc tcagttcgcc gaagtaggta tagcagttca gtgcccagcc taactgcgca
    36841 tgttccggca cgtgggactg aacaaaaatg gtgcttttct caggatcgat accacaagcc
    36901 agatacaagg ccagcgtatc cagcgtcgct ttacgcagct tctgtgcatc ctggcgcacg
    36961 gtgatcgcgt gttggtcaac gatacagtaa atgcaatggt agtcatcctg catgtttacc
    37021 cactgacgca gcgcacccat gtagttacca atggtcaatt cacctgaggg ctgtgcgcca
    37081 ctaaaaacga tgggcttagt catttttcga ttcctgattt tcgctatgcg gaagccctaa
    37141 tgcgggcaga aggtcattta tagactgata aattacatca ggctggctga gatcgatagc
    37201 ctcgccgtag ttatatccgt aggttaagcc aactgatggg caacctgccg cttttgctgc
    37261 ctgaatatca ttgcgtgagt cgccaacaaa cagcatctgt tgtggagcaa ttcccatccg
    37321 ctcagccacc agtaacagcg ggtccggatg cggttttttg ttttgcacat catcaccacc
    37381 aattaccacg ctgaagtatt tggcgatatc taaggcttcg agcagcggcg cgacgaacgg
    37441 cgtcggtttg ttggtgacca gacctagcgg caggccttta gcctgcaatg cgcccagcgt
    37501 atcggcaacg tgcgggaaca aaaacgtccc ctcttcggca acctcgccat agtagcgatc
    37561 gaacagttta cgcagaatac gtacttgttc ttctgccgga atgtcgtcat caacgggcgg
    37621 tttacccatt gttttacgca gagtcgcacg ttcctgacgc gcccaggtca atgcgcgctc
    37681 catcagaaca tctgcgccgt taccaatcca ggttataacg cgttcttcac ctgcgacggg
    37741 caactccagc gcatacagcg ccatatctac tgcggtagcc aagccaggag cactgtccac
    37801 cagcgtaccg tcgagatcaa aagcgacgcc gcgaatatct tcaaacttat tcatgactta
    37861 cctttgccag ttcactgcgc atttcatcaa tgactttttt gtagtctggc tggtcgaaga
    37921 ttgccgaacc agcaacaaac atatccgcgc ccgccgcagc gatttcgcca atgttgttca
    37981 ccttcacgcc accgtccact tccagtcgaa tgtcaaagcc agactcgtcg atacgacgac
    38041 gtacttcgcg cagtttatcc agtgtttgag gaatgaaaga ctgaccgccg aaaccaggat
    38101 taacggacat cagcaggatc acatccagct tatccatcac gtaatccaga tagctcagcg
    38161 gtgtcgccgg gttaaatacc agccccgctt tacagccatt ttctttaatc agttgcagcg
    38221 tgcggtcaac atgctcggag gcttctggat gaaaggtaat gatgctggca ccagcggcag
    38281 cgaaatcagg cacaatgcga tcgacgggtt tcaccatcag atgtacgtcg ataggggcgg
    38341 taatgccgta gttacgcaag gatttcagca ccattggccc aatcgtcaga ttgggaacat
    38401 agtggttatc catgacgtca aaatgcacga catcagcgcc agctgctagg gcttttgcgg
    38461 tatcttcacc caggcgggca aaatcagccg acagaattga gggggcaatc aaatactgtt
    38521 tcatccgctt ctccttgaga attatttttt cgcgggtgaa acgactcctg gtttgtacaa
    38581 agccagcagt tcgtccacct ttttacgtgt gccgccgttg ctgcttatac tgcgtcgaac
    38641 tttgacgaca tgcaattttg cgcgctgata ccactcacgc gttaacatcg tatcgtgatt
    38701 ggagatcagc acaggaatat ggcgatcaac cagaccttcg gcgatctccg ccagatgcgc
    38761 ttgttgttca agcgtaaaac tgtttgtgtg atacgccgta aagttggcgg tcgcagacag
    38821 cggtgcataa ggcggatcgc aatagacgac ggatgcatca tctgcgcgtg ccatgctatc
    38881 ggcgtaagac tcacaataga aaaaggcatt ctgcgctttt tcagcgaagt gatacaactc
    38941 tgcttccggg aaatagggtt ttttgtagcg gccaaacggc acgttaaact caccgcgaag
    39001 attgtaacga cacaggccgt tgtaaccgta gcggttcaaa tataaaaaca gtaccgcccg
    39061 acggaacgga tcctggcttt tgttgaactc ttcgcggaac tgatagtaaa cctcggcgca
    39121 atttgtttcg ggaacaaaca gctcgcgtgc ggcctgtacg tactcatcag tacgcatttt
    39181 cacaatgtta tagagactga tcaggtcgct attgatatcg gcaaggatat aacgagaaaa
    39241 gtcggtgttg agaaacaccg acccggcacc tacaaaaggc tcaaccagac attcgccctt
    39301 gggcaaatgc cgtttaatat catcaagcag gggatacttg ccccctgccc acttcaaaaa
    39361 agcgcgattt ttcttcatgc tgactaacta attacacctt ctccggctgt ggagaaagct
    39421 ccgacagcat ccagcgcttc aggcaactcc cgcgatgata aaccattgcg ggagataacc
    39481 ttgattactt cagatcggcc tgtacctgac gcagcggttt cgcccacggg tttttggcct
    39541 ggacatcggc tggcaatgta gataccgctt ttttcgcctc ttctttcgaa gcgtacacgc
    39601 cagaaaccag gacataccac ggctgaccat tacgcgtcgt ttcatagaca acgtagtttt
    39661 tcagattctc tttcttcgcc caaccgttca ggttgtcgta gttagaggaa ctgctcagct
    39721 gcagagtgta atggctggac ggtgccgatt tcaacgaacc cacattacct gcggtcttcc
    39781 ctccagcggc tggtgttgcc gtggtttgcg ccggggatgc cgtctgtact ggagccgtag
    39841 ttgcggtctc ttttggtgct ggcgtagagg tcgcagccgg tgccttcgtg cttgccacag
    39901 gagcagctgg ttcagtacgc ttcggcgtct gtgctaccgg cttcggctcc gttttcacgg
    39961 ttgcttgcgg ttttttcggt tcaatcaccg cctgctggcg agctggacgc gtagtggccg
    40021 gacgttcagc ggtttgcgtt ttcgccgtgt cacgcgatgc attgccattg cgaacaggcg
    40081 cgaccgtcgc gggttcagtc ggcaacgtgg aattgaccgc cacattattc aactgttgct
    40141 gattttgtgg ctgggtcagc gcattgttca ggtcaccctg cacttcaaca cgttgttgac
    40201 catccgttgc cgccggggtt tgcccttgag tcggcgtaga agagatcggc ggcagagaaa
    40261 catcctgctg agtattttcc gcagacgtgg ttcccggcgc tggctgcaca ccattcgcct
    40321 gatcggtcgc attgcctgca agatcaatac tcttctcgcc agacgcggtt tgatcgctgg
    40381 aagaggtcga gggggctttt agcgcagaac cgataccgat gatcaacagt aggagaacca
    40441 gaatgccgac ccccatcatc atatactgac gagaagcggg tttactggct gcttttttgc
    40501 gcttacgcgg acgacgctct acgcgctctt catccacgat ttcatcttcg gattcgtcaa
    40561 tttcttcttc gatttccggc tcatcattgc gctctttttg cgcacgagtc ggacggcgat
    40621 cgtcagtgtc atcaagttca atatcatcaa aattgatctg cggttcgcca cgttcagtac
    40681 gctcagaacg ttcagaagat tgacgagaac gaccagtacg acgatcgctg ggatcgggtt
    40741 tcagctcgtc ttctggtttg aattcatcca tttaacaccc cactaaaagg ttaatgctta
    40801 ccacgttgca attaacctga agctaataga ccgcttgata agcggcctga cctttcttgt
    40861 tgttacgctg attgacaatc ggcaatggcg ttaagaacaa gctcgtgcga aacgccgctg
    40921 cgaacttcac tcttaccaat tgccaacgga agaattaagc gcatctctcc cgcaaggact
    40981 ttcttgtcac gcagcatatg cggtaaatac gcctgcgcag acatttcgcg cggcccattg
    41041 accggtaacc cagcccgcgt gagcagggtt ataatacgct gcgtttcggc agaactaaac
    41101 tgcccgagac gttccgacgt ccgcgccgcc atcaccatac ccgcagcaac cgcttcaccg
    41161 tgtaaccaat tgccataccc catttcagct tcaatggcat gaccaaaggt gtgtcccaga
    41221 ttcagtaaag cacgtaaccc ggtttcgcgc tcgtcggcgg cgacaacttc tgccttcagt
    41281 tcacaacaac ggcgaataca gtacgccatt gccggaccgt ccagacgcaa caacgcatcc
    41341 agattctctt ccagccagtt aaaaaacgca ccgtcaagaa taatgccgta tttgatgact
    41401 tctgccagcc ccgacgctaa ctcacgcggg ggaagcgttt tcagacagtc gagatccacc
    41461 accactgaag caggctggta gaacgcgcca atcatgtttt taccgagggg atggttgacc
    41521 gcagttttgc cgccaacgga ggaatcgacc tgcgacagta acgtcgtcgg gacttgaatg
    41581 aaacgtacac cgcgctgata actcgccgcc gcaaagccgg tcagatcgcc cactacgccg
    41641 ccgccaagcg ccaccagcgt agtatcgcga ccatgcggct tttgtaacaa cgccgtaaag
    41701 acggtatcga gtacagccag gcttttatac tgctcgccgt cagggaggat aacgctatcg
    41761 acgttaacac ccgcctgttc aagtacgccg cggaccttat cgagatacag aggagccagg
    41821 gtttcgttgg taaccaacat gacctgctcg cccgatttca gcggtaagaa tgaagctggt
    41881 tcattaaaca aaccagatgc gatggtaatt gggtaactac gttccccgag agtaacgaca
    41941 atcctctcca taacgcgaca tccaccttaa ttactgtacc cgcagacgag tgtatataaa
    42001 gccagaatta gttgctttcc agcatgtgaa taatctggtt tgcgaccact ttagcgcttt
    42061 gatcatcagt acgaatggtc acgtcggcaa tctcttcata cagcggattg cgttcattgg
    42121 ccaacgcttc cagaacttca cgcggcggtg tttcaacgtg cagcaacggg cgttttttat
    42181 cacgctgcgt gcgtgcaagt tgcttttcga tggtcgtttc aagataaacg acaacgccac
    42241 gagcggaaag acggttacgc gtttcgcggg atttcacaga gccgccgcca gtagccagca
    42301 caataccctg tttctcggtc aactcattga tgaccttttc ttcgcgatcg cggaagcctt
    42361 cttcgccttc taaatcgaaa acccagccca catcagctcc ggttcgtttc tcaatctctt
    42421 gatcggaatc gtaaaattcc atattgagtt gttgagctaa ctggcgccca atagtgcttt
    42481 ttccggcacc cataggccca accagaaaga tattgcgttt ctctgccatt ttttcggtac
    42541 tactaagact attcgttaat gataaacccg cttcgctcag agagcgccgc aggacatgaa
    42601 ctgaaacctc ataagatatt gcgagagtca gactgaaaat tatctcaata ctccagcggg
    42661 tttggcaact gaataaatca cccatccctc tgcatatctg gtcgctgcaa gcgcgctgcc
    42721 ttgctaccac cgctctggcg ataaatcacc gggtaagatt agcgtaaaaa agacagcaaa
    42781 atgccgcctg aatgataaat catcatcatg gaacgcaggc agcgaagagc accaactctc
    42841 aaatcggtac tccttgtatg ctaaatacct gcgcgcgtca aatagatgaa acacgttcag
    42901 cgtaaaaaca ttaccgcttt tacggctgtt tactcactgg aaaccagtcg tggcgtgata
    42961 aacaccacta actcgcgtcg ttcatcttct tttccgtcat gacgaaataa ttgcccgaac
    43021 cagggaatgt cgccaagcaa cggtacgcta tcctgacccg atttattttt acgggtaaaa
    43081 atgccgccca gcgccaacgt ttctccgctt ttgacctcga cctgcgtttc gatctcctgc
    43141 ttatcaatcg ccagcacttc gccatcggcc tgctgtagca cctgccccgg aacgttctgg
    43201 ctgatgtgta atttcagccg gatgcgacct ttttgtaaca ccgtgggcgt gacctccatc
    43261 cccaggacgg cctctttaaa ttccaccgac gtcgcgccac tttccccgct ggaaacctga
    43321 tatggaattt cgctcccctg tttaatgctg gcaggctgaa gatgtgaggc cagcagacgc
    43381 ggactggcga taatatccag ctgctgtttt tgttcgagcg cagaaagctc aagatccagc
    43441 aaacgtccgt tgatacgccc aatgttaaaa ccgacatgcg ttgtcgccgt cgctacggag
    43501 aggtcgctgc caagcgtggt gacttgccca acgccaccag cttgttgcgc atcggccagc
    43561 gtccatttca cgcctaactc acgcaaactt ttttcattaa tggtgacaat atgcgccgac
    43621 agctcaactt gcccgaccgg cagatccatt tgcgctaccc actgttcaag ggcgcttaac
    43681 gccgttttgt tatctcgcag caaaaggcga ttggtgcgtt tatcgacggt catactccct
    43741 ttggcactca gtagcttctc cccagctttc gccagctctc cggcgtcggc gtattgcaga
    43801 gtaatattgc gattttccag cggcagattt gcctgcgccc gcgcctgctc cgcttcctga
    43861 cgggcgatat tgtcattctg ccaggcaatg gaatgcactg agagaatgtt gccctcctgg
    43921 cgcgttatca gtccggcgct tttcactaca gtttgtagtg cctgcttcca gggaacatct
    43981 gttagatgta acgacaccgt accgctgacg tctggcgaca ccaccaggtt caacttctcc
    44041 tgttcagcca gcgcctgcaa cacctgagct accggaacgt catccaccat cagtgtcact
    44101 ttttgcggct ttgccgcctg tacgccgggt atcagcatca acagtagtgc ggctatccat
    44161 tgcttcattt gtatctcctt gccgttgcca caaccattgt ggcggttcgc agtttgttcc
    44221 ggttcccagc gttagtgcgt ctggcgtcag ctgtaaaatt gtccagccgt tttccagcac
    44281 atcgttttgc tgcacccgtc gccatttctt ttgcccgtct tttattacac cgatgatgcg
    44341 ctcgcctcgc cctactatcc cctgatagcg ccactggcta agttcgctaa tccggcatag
    44401 atcttccggc ggtttaaaag ggtcacgcat accggttaaa aggcacaatg caatacctgc
    44461 caacaaccag cgtttacccc tcatgcggcg tctccagttg tagcgtgaac aaaagatcat
    44521 caccttccac gcttaacgaa aaacggctca cgctgacgtt gcgctctgcc agccgtgtaa
    44581 atgccgacgg cactgcttcc cacagcgttt tcaacgccaa ctcacctccc tgcgcggatg
    44641 gatgccagga aaccagttgc gcgccgggca actgaaaatc cagtggcgaa aagggcagcg
    44701 ttttttcttc gctgaaggaa gtggtgtcta ccaggcgata cagtgcggcc cactgctggt
    44761 gatgactcgc ccgtagtcga attaatgcgt cgctctcttc ctggtgtgtc gatgacagaa
    44821 aaatgagcgt aactaacatc atcaaccaga atgcccagca aagctggcgg aggcggggtg
    44881 atgcggcgaa ccaccagtca aagagcatgt ccatcgctaa ccttccttgt taactgatac
    44941 tcaaattgcc agcgtccctg cgcatcctgc tgcgtggccc cccgctgatt gagatgaaaa
    45001 gatgcatcct ggcggagtga cgtttctagt gcgtttaacg cggtaatgct tgttgtcagc
    45061 cccttgatct ccagcgttcc ctgctgccag cttatcgttg tcagccaggc gtgctcgggt
    45121 aaaagcgccg ccagtgcttc cagcgcagat tgccagtcgc gggtaaattg tcgctggcgc
    45181 tgcctttgca aacgctgttc gcgtaactgc tgttgctcca gcaaacgtgg tttcgttatc
    45241 tgtaagctgc gagcgagttg ttgttccgcc tgaagcaaaa cggcgtttac gcgtgcttcg
    45301 gcgttgcttg tcagacgcat tattagcgta atcccgacgg ccagcagcag aggcgcaacg
    45361 aacatcagca accagaaacg cagaaaagcg gtccggcgtt gctgtcgcca gggcaaaaaa
    45421 ttaattggcg ggttcatcag ttcgccttcc ctagcgccag ccccagcgcg atggcaaagt
    45481 ctccacctgg cggcggtagc ggcggctgac gaacagaaac ggcctcccag ggatcaaatc
    45541 cgccttcgcc acatagcgcg acgctttcgg gatcaacgga taacgctgcc gccagctcct
    45601 tcgcgctagt catccccacc gccagtttgc gcccccagcg atagcgcgtc gcccacagcc
    45661 actgttcgtt atcacgccag gccagacatt gctgatgaga aggtaaaaaa ggcaggaatc
    45721 gctgtaatgc actggcatcc ggggtgatcg cgctaacatg aacacgcaac ctttctgcca
    45781 gagtaagcag cgttgccagc tctttgcttt gcgcggcagt cacgttatag gcgggactga
    45841 gtgagtcttc gctgtaatcg aagcgcaggg agtccggatc catatccagt tcacgggcca
    45901 tcgtcccgga cagccaggcc gtttgctccc gctcaccaag ggacatcgac gggcgcggaa
    45961 atgaccgctg taatgtgcga ctggcgggaa acgccaacat aatgtgatga cgctgcggca
    46021 gttcgcggct ccacggtaac aacgtgttag ccagccgctg cgcatcaaca atccgcccat
    46081 ctttgataat gtcattctcc agcggcaacc gccaccagcg ttgcaaaaag cattcttttg
    46141 cgccccgcac gatcgcaacc gctaccgctt cttgctgttg taaatgcaaa ccaatttgcc
    46201 agatcttaaa tgccattgtg atgatctcct tatcacccgt cactctgacg ggtatatcaa
    46261 tgcgtctggc ttgcctttat actaccgcgc gtttgtttat aaactgccca aatgaaacta
    46321 aatgggaaat ttccagtgaa gttcgtaaag tattttttga tccttgcagt ctgttgcatt
    46381 ctgctgggag caggctcgat ttatggccta taccgctaca tcgagccaca actgccggat
    46441 gtggcgacat taaaagatgt tcgcctgcaa attccgatgc agatttacag cgccgatggc
    46501 gagctgattg ctcaatacgg tgagaaacgt cgtattccgg ttacgttgga tcaaatcccg
    46561 ccggagatgg tgaaagcctt tatcgcgacg gaagacagcc gcttctacga gcatcacggc
    46621 gttgacccgg tggggatctt ccgtgcggca agtgtggcgc tgttctccgg ccacgcgtca
    46681 caaggggcga gtaccattac ccagcagctg gcgagaaact tcttcctcag tccagaacgc
    46741 acgctgatgc gtaagattaa ggaagtcttc ctcgcgattc gcattgaaca gttgctgacg
    46801 aaagacgaga tcctcgagct ttatctgaac aagatttacc tcggttaccg cgcctatggt
    46861 gtcggtgctg cggcacaagt ctatttcggt aaaacggtcg accaactgac gctgaacgaa
    46921 atggcggtga tagccgggct gccgaaagcg ccttccacct ttaatccgct ctactcaatg
    46981 gatcgtgccg tcgcgcggcg taacgtcgta ctgtcgcgga tgctggatga agggtatatc
    47041 acccaacaac agttcgatca gacacgcact gaggcgatta acgctaacta tcacgcgccg
    47101 gagattgttt tctctgcgcc gtacctgagc gaaatggtgc gccaggagat gtataaccgt
    47161 tatggcgaaa gtgcctatga agacggttat cgcatttaca ccaccatcac ccgcaaagta
    47221 cagcaggccg cacagcaggc agtacgtaat aacgtgctgg actacgacat gcgccacggc
    47281 tatcgcggcc cggcaaatgt gctgtggaaa gtgggcgaat cggcgtggga taacaacaag
    47341 attaccgata cgttgaaggc gctgccgacc tatggtcctc tgctgcctgc cgcagtcacc
    47401 agcgccaatc ctcaggaagc gacggcgatg ctggcggacg ggtcgaccgt cgcattgagt
    47461 atggaaggcg ttcgctgggc gcgtccttac cgttcggata ctcagcaggg accgacgccg
    47521 cgtaaagtga ccgatgtttt gcaaacgggt cagcaaatct gggttcgtca ggttggcgat
    47581 gcatggtggc tggcacaagt gccggaagtg aactcggcgc tggtgtcgat caatccgcaa
    47641 aacggtgccg ttatggcgct ggtcggtggc tttgatttca atcagagcaa gtttaaccgc
    47701 gccacccagg cactgcgtca ggtgggttcc aacatcaaac cgttcctcta caccgcggcg
    47761 atggataaag gtctgacgct ggcaagtatg ttgaacgatg tgccaatttc tcgctgggac
    47821 gcaggtgccg gttctgactg gcagccgaaa aactcgccgc cacaatatgc tggtccaatt
    47881 cgcttacgtc aggggctggg tcagtcgaaa aacgtggtga tggtacgcgc aatgcgggcg
    47941 atgggcgtcg actacgctgc agaatatctg caacgcttcg gcttcccggc acaaaacatt
    48001 gtccacaccg aatcgctggc gctgggttca gcgtccttca ccccaatgca ggtggcgcgt
    48061 ggctacgcgg tcatggcgaa cggcggcttc ctggtggacc cgtggtttat cagcaaaatt
    48121 gaaaacgatc agggcggcgt gattttcgaa gcgaaaccga aagtagcctg cccggaatgc
    48181 gatattccgg tgatttacgg tgatacgcag aaatcgaacg ttctggaaaa taacgatgtt
    48241 gaagatgtcg ctatctcccg cgagcagcag aatgtttctg taccaatgcc gcagctggag
    48301 caggcaaatc aggcgttagt ggcgaagact ggcgcgcagg agtacgcacc gcacgtcatc
    48361 aacacaccgc tggcattcct gattaagagt gccttgaaca ccaatatttt tggtgaacca
    48421 ggctggcagg gtactggctg gcgagcaggt cgtgatttac aacggcatga tataggcgga
    48481 aaaacgggta ccactaacag ttcgaaagat gcgtggttct cgggttacgg tccgggcgtt
    48541 gtgacctcgg tctggattgg ctttgatgat caccgtcgta atctcggtca tacaacggct
    48601 tctggagcga ttaaagatca gatctcaggt tacgaaggcg gtgccaagag tgcccagcct
    48661 gcatgggacg cttatatgaa agccgttctt gaaggtgtgc cggagcagcc gctgacgccg
    48721 ccgccgggta ttgtgacggt gaatatcgat cgcagcaccg ggcagttagc taatggtggc
    48781 aacagccgcg aagagtattt catcgaaggt acgcagccga cacaacaggc ggtgcacgag
    48841 gtgggaacga ccattatcga taatggcgag gcacaggaat tgttctgatt aaaaaggcgc
    48901 ttcggcgcct ttttctgttt gctgacaaag tgcactttgt ttataccgga tacggcgtga
    48961 acactttacc cgtctggcaa catctagaaa aatcaatata ttgcaggagc cgcacaggcc
    49021 tgataagcat tatgcatcag gcaaacttca cgcatttaca accgcccctg ccccttcaac
    49081 cattcgcgca cgaggaacag cgcgctgaca ttacgcgctt cattgaagtc aggatcttcc
    49141 agcaaatcca tcatatgcgc cagcggccag cgcacctgtg gtagcggctc tggctcatcg
    49201 ccttccagcg attccggata gagatcttgc gctaccacga tattcatttt gctggaaaag
    49261 taagacggcg ccatgctgag cttcttcaaa aaagtcagat cgttcgcacc aaatcccact
    49321 tcttctttta gctcgcggtt agcggcttcg tagacgcttt cacccggatc aattaatcct
    49381 ttcgaaaaac ctaattcgta ggattccgtt cccactgcgt attcgcggat caggatcagg
    49441 tgatcgtcca caatcggcac aatcatcacc gcttcccggt tggttggacg cattcgttca
    49501 taaacacgcc gcacgccatt gctgaactcc agatccacgc tttcgacggt aaacagtcgt
    49561 gaacgggcta cagtttcaac attcagaatg gtgggttttt gtaatgattt gctcatcgtg
    49621 ggatctatgc tgtgaaatca gcggttattg tgcgatatcg gacacgcttt cggcaatgtg
    49681 aattgcatgt tatttacatt tatgtaactt attaaataat catcctcaaa tcaaattaaa
    49741 agtcaatagg ttgaaataac tccaggaatt tgctgatatt ccgccttcag agggtttgct
    49801 atgatcagcg gttactgtga tgtgcttaat gatgctcaag ttaaactcca cgcttgccga
    49861 tagccaaccg cagaatcatg tattgtgtcc ggtgcgactg accacgcctg acagactaag
    49921 taagatgggg aaagcatgag caccattgtg atttttttag ctgctttgct ggcctgctca
    49981 ctacttgcgg gatggctgat aaaagtgcga tccagacggc gtcaactgcc ctggaccaac
    50041 gctttcgcag acgcgcaaac gcgcaaactc acacctgaag aacgtagcgc cgttgaaaat
    50101 tatcttgaga gcctgacgca ggtattacag gtgcctggcc caacgggagc cagcgcggca
    50161 ccgatctctc tggcgctgaa tgccgaaagc aacaacgtca tgatgctaac acacgctatc
    50221 acgcgttacg gcatctctac cgacgatccg aataaatggc gttactacct cgattcggta
    50281 gaagtccacc tgcccccttt ctgggagcag tacatcaacg atgagaatac cgttgaactg
    50341 attcataccg attcgctgcc gctggttatt tcactcaacg gtcatacgct gcaggagtac
    50401 atgcaggaaa ctcgcggcta tgccttgcaa cctgttccgt caacacaggc gtcgattcgc
    50461 ggggaagaga gtgagcaaat cgagctactc aatattcgca aagaaacgca cgaagagtat
    50521 gcgctaagtc gtccgcgcgg gctgcgcgaa gcgttgctga ttgtagcctc cttcctgatg
    50581 ttctttttct gcctgattac cccggatgta tttgttccgt ggctggcagg cggcgcgtta
    50641 ctgctgctgg gcgcaggact gtgggggcta ttcgcgcccc cggcaaaatc ctccctgcgg
    50701 gaaattcatt gtctacgcgg cacaccccgt cgttggggat tgtttggcga aaacgatcag
    50761 gaacagatca acaatatttc actcggtatt atcgacctgg tctatcccgc acactggcag
    50821 ccctacattg ctcaggatct tggtcaacaa accgatatcg atatctatct cgaccgccac
    50881 gtagtgcgtc aggggcgata tctttcgctg catgatgaag taaaaaactt tccgttacag
    50941 cactggctgc gcagtacgat tatcgctgcg ggctcgctgc tggtgctgtt tatgctgtta
    51001 ttctggatcc cgctggatat gccgctgaaa ttcaccctct cgtggatgaa aggcgcacag
    51061 accattgaag ccaccagcgt aaaacaactg gctgacgctg gcgtgcgggt tggcgataca
    51121 ttgcgtatta gcggtacggg aatgtgtaat attcgcactt ccggaacctg gagcgcgaaa
    51181 accaattcac cttttttacc gtttgactgc tcgcagatca tctggaatga cgcccgctca
    51241 ttgccattac cagaatctga actggtcaac aaagcgacgg ctttgaccga agcagttaat
    51301 cgccagctgc accctaaacc ggaagatgaa tctcgcgtca gcgcctcatt acgttcagca
    51361 attcaaaaat ccggcatggt attgcttgat gattttggcg acattgtact gaagacagcg
    51421 gatttatgtt ctgccaaaga tgactgtgtg cgactgaaaa atgcgctggt caatctcggc
    51481 aacagtaaag actgggacgc gctggtaaaa cgcgccaacg ccgggaaact cgatggcgtg
    51541 aatgtgttat tacgcccggt gagtgcggaa tcactggata acctggtggc aacctccacc
    51601 gcgccgttta tcacgcatga aacggcgcga gcggcacaat cactaaacag tccggccccc
    51661 ggtggattcc tgattgtcag cgacgaaggc agcgattttg ttgatcagcc ctggccttcg
    51721 gcatcacttt acgactaccc gccgcaagaa cagtggaacg ctttccagaa actggcacaa
    51781 atgctgatgc atacgccgtt taacgccgaa ggtatcgtca caaaaatctt cactgacgcc
    51841 aatggtacgc agcatattgg ccttcatccg atcccggatc gttccggcct gtggcgctat
    51901 ctcagcacca cgctgctgct actgacgatg ctgggtagcg ccatttataa tggcgtacag
    51961 gcctggcgtc gttaccagcg tcatcgcact cgcatgatga agattcaggc ctattatgaa
    52021 agctgcctga acccgcaact gatcacccct tcagaaagcc ttatcgaata acacgtttgc
    52081 gcggcaggtt atgctaccct gtcgcgcaaa ttgcttcact ctggagattt ccctcatgca
    52141 tatcaacatt gcctggcagg acgtagatac cgttctgctg gatatggacg gcacgttgct
    52201 cgacctcgcc ttcgataact atttctggca aaagctggtg cctgaaacat ggggcgcgaa
    52261 aaacggggtt acgccacagg aagcgatgga atatatgcgc cagcaatatc acgacgtaca
    52321 gcatacgcta aactggtact gtcttgatta ctggagtgag caactgggtc tggatatctg
    52381 tgcgatgacc accgagatgg gaccgcgcgc cgtactgcgt gaagatacca ttccgtttct
    52441 tgaggcactg aaagccagcg gtaagcagcg aattttgctc accaatgcgc atccgcacaa
    52501 cctggcggta aaacttgagc ataccggtct ggacgcacac cttgatttat tactttccac
    52561 ccacacattt ggttatccga aagaggatca gcggttatgg catgcggtgg ccgaagctac
    52621 gggtcttaaa gctgaaagaa cgctgtttat tgatgacagc gaagcgattc tcgatgctgc
    52681 cgcgcaattt ggtattcgtt actgcctcgg tgtgactaat cctgactccg ggattgccga
    52741 gaaacagtat caacgccatc cgtcactgaa tgactaccgc cgcctgatcc cctcgctaat
    52801 gtgaaggaga tgccatgaaa gaaaaacctg ctgttgaggt tcgactggat aaatggctat
    52861 gggctgcccg tttttataaa acccgcgcgc tggcccgtga aatgattgaa ggcggtaagg
    52921 tgcattacaa cgggcagcgc agcaagccaa gcaaaatcgt cgagctgaat gccacgctca
    52981 ctctgcgcca gggaaatgac gaacgcacgg tgattgtaaa ggcgattact gaacagcgtc
    53041 gccccgccag cgaggcagcc ttgctgtatg aagagactgc ggaaagtgta gagaaacgcg
    53101 aaaaaatggc gctggcacgt aaacttaatg ccttaaccat gccgcatccg gaccgacgcc
    53161 cggacaaaaa agagcgccgc gacctgttac gatttaaaca cggcgacagt gaataactgt
    53221 cacctgcaag agagatgatt atgccgcaac atgaccaatt acatcgctat ctgtttgaaa
    53281 actttgccgt tcgcggcgaa ctggtaaccg tttcggaaac cctgcaacag atccttgaga
    53341 atcacgatta tccgcagccc gttaaaaacg tgctggcaga actgctggtt gcgaccagcc
    53401 tgttaaccgc tacgttgaag tttgatggtg atatcaccgt acagctgcaa ggcgacggtc
    53461 cgatgaatct ggcggttatt aacggtaaca acaaccagca gatgcgcggt gtagcgcgcg
    53521 tgcagggcga aattccagaa aatgccgacc tgaaaacgct ggtcggcaat ggttacgtgg
    53581 tgatcaccat caccccaagc gaaggcgaac gctatcaggg cgtggtgggt ctggaaggtg
    53641 ataccctggc ggcctgcctg gaagattact ttatgcgttc tgaacagttg ccgacgcgcc
    53701 tgtttattcg caccggcgac gtagacggca aaccggctgc aggcggtatg ttgttgcagg
    53761 taatgcctgc gcaaaatgcc cagcaggacg actttgacca cctggcgacg ctaaccgaaa
    53821 ccatcaaaac cgaagaactg ctgaccttac cggcaaacga agtgttgtgg cgtttgtatc
    53881 acgaagaaga ggtgacggtt tacgatccgc aggatgtgga gttcaaatgc acctgctcgc
    53941 gtgaacgttg cgccgacgcg ctaaaaacgc tgccggatga agaagtcgat agcatcctgg
    54001 cggaagatgg cgaaattgac atgcattgtg attactgcgg taaccactat ctgttcaatg
    54061 cgatggatat tgctgaaatc cgcaacaacg cgtctccggc agatccgcaa gttcattaat
    54121 gagtatgtcc ggcagagagg gctctgccgg attttacgac gtaatggcct gatgcgctgc
    54181 gcttaccagg cctatgaaat aaatcgcaag ttattttggt cagatacagc gtcgtatccg
    54241 ttaaaagcgt tgtcacttac ttaagtttat ttccagcgtt tcaccatcta ccgtcagatc
    54301 gcgccacgct ttaggcctcg tccccagctt tccatccttc gcagcagcga gtaatttttc
    54361 tttcgttatt tggcgcggaa gtaaactcat atcaagcgcg tcccattctt ttctatctga
    54421 gtcaaagcca tagacaatga ctctgtcatc attaaaagca aacaggatcc gctccgcctg
    54481 accatcacta ttcagatctt gctcgaccag tacgcaggcg tctttttcaa tacaggtcat
    54541 cacgttaaag cgatcctgga ttaaggccga ccagaatgtc gcatccggtt taccagaacc
    54601 tggggcaatt aacacattat cggctaatac tttttccgat acctgttcct gaagatgttg
    54661 ctctccatct aacgccatca gcagatcccg cgcgcgtttc gggtctttca taaacccggc
    54721 atcgcttttc agcgactcaa gcgccgcacg tccatagcga ccgctctgtt cgagcatgta
    54781 gatagttacc tggtctggcg tgtttttgcc gctctgataa cgcgccatat gactgttcac
    54841 actaatgcgc atactatcca gcaccggcga attaagaagc accagtatta ccaacaccaa
    54901 taacgaaacc gcaaggttca ctttgccctg aagaacaaga gggttttgct ctttacgcca
    54961 gacgatactg acaaaatacc ccagcgacca taccagtaac accagcaccg ccagcgcgcc
    55021 ctgcaagcgg tcgacagtcc agccgtactg agcgacccgc agccataacg cccaggcggc
    55081 gacgaacaca tacagcggag cagccagcag agcggtttta atcaggcaac gcaacggccc
    55141 cgtccaggga agtgacgctt tttgcggatc gcggacaata gccattaaga tcaattgcaa
    55201 aaaggccagc gttaacagca acccagcggc ggagatgtgg cgagaaatcg cgctcaggcc
    55261 cgtaaacggc agggtgataa taaacatcag ggttagcaat gatactaacg gcagcaaccc
    55321 cgtggcgatt aacgtgaaca acttttgaat agagtcgatt aaacgtgact gtgttcgcgc
    55381 gaggatcacc gccagcgcgg taaccagacc taacgttaga taaataaacc agtcggttgc
    55441 aaaaaagagc gttttgaaaa acgtgatgcc aacaagttta aacaactcac tccacagtaa
    55501 aagcaccagc cacgtcaggc cattggcgag aaaaatcacc agtaatataa gtacgttatg
    55561 ccatactgac tggtaaaaat agcgatagcg gctactgtca ttgcggatgc gaaggctttg
    55621 ttgtatccac ggtagcaaca acattgccat cagcagcaga tagcagccaa aatcccaaat
    55681 cgccttttca gctctccagg gggtcatgcc atcaatttgc cactttagcc aaccgctcat
    55741 ccctagcgtg gcaataaaca ccagcgccag ccatccccaa aggcgcttct gtttaaagga
    55801 gatcacggag aagagtaaaa cggatgaaaa cgcgacggtc gcaggcacgc cataaacaat
    55861 ccaactgaga ttttttcctg caagccaggc gatcagcagg tagcaaacca gaccctgaag
    55921 taatccggta gcaatcatcc cccaacgtgt tgcgggtgaa agttcaacgc tatccatgtt
    55981 agttatccag aatcaaaaag tgggttaatt atcgcatcca ggccgtagta ttttgctttt
    56041 ttcagaaaat aatcaaaaaa agttagcgtg gtgaatcgat actttaccga ttgaatttgc
    56101 atcaatttca ttcaggaatg cgattccact cacaatattc ccgccatata aaccaagatt
    56161 taaccttttg agaacatttt ccacacctaa aatgctattt ctgcgataat agcaaccgtt
    56221 tcgtgacagg aatcacggag ttttttgtca aatatgaatt tctccagata cgtaaatcta
    56281 tgagccttgt cgcggttaac acccccaaaa agactttact attcaggcaa tacatattgg
    56341 ctaaggagca gtgaaatgcg cgttaacaat ggtttgaccc cgcaagaact cgaggcttat
    56401 ggtatcagtg acgtacatga tatcgtttac aacccaagct acgacctgct gtatcaggaa
    56461 gagctcgatc cgagcctgac aggttatgag cgcggggtgt taactaatct gggtgccgtt
    56521 gccgtcgata ccgggatctt caccggtcgt tcaccaaaag ataagtatat cgtccgtgac
    56581 gataccactc gcgatacttt ctggtgggca gacaaaggca aaggtaagaa cgacaacaaa
    56641 cctctctctc cggaaacctg gcagcatctt aaaggcctgg tgaccaaaca gctttccggc
    56701 aaacgtctgt tcgttgtcga cgctttctgt ggtgcgaacc cggatactcg tctttccgtc
    56761 cgtttcatca ccgaagtggc ctggcaggcg cattttgtca aaaacatgtt tattcgcccg
    56821 agcgatgaag agctggcagg tttcaaacca gactttatcg ttatgaacgg cgcgaagtgc
    56881 actaacccgc agtggaaaga acagggtctg aactccgaaa acttcgtggc gtttaacctg
    56941 accgagcgca tgcagctgat tggcggcacc tggtacggcg gcgaaatgaa gaaagggatg
    57001 ttctcaatga tgaactacct gctgccgctg aaaggtatcg cttctatgca ctgctccgcc
    57061 aacgttggtg agaaaggcga tgttgcggtg ttcttcggcc tttccggcac cggtaaaacc
    57121 accctttcca ccgacccgaa acgtcgcctg attggcgatg acgaacacgg ctgggacgat
    57181 gacggcgtgt ttaactttga aggcggctgc tacgcaaaaa ctatcaagct gtcgaaagaa
    57241 gcggaacctg aaatctacaa cgctatccgt cgtgatgcgc tgctggaaaa cgtcaccgtg
    57301 cgtgaagatg gttctatcga ctttgatgat ggttcaaaaa ccgagaacac ccgcgtttct
    57361 tatcctatct atcacattga aaacatcgtt aagccggtgt ccaaagcggg ccacgcaact
    57421 aaggttatct tcctgactgc tgatgctttc ggcgtgctgc cgccggtttc tcgtctgact
    57481 gccgatcaaa cccagtacca cttcctctct ggcttcaccg ccaaactggc tggtacagag
    57541 cgcggcatca ccgaaccgac gccaaccttc tccgcctgct tcggcgcggc attcctgtcg
    57601 ctgcacccaa ctcagtacgc agaagtgctg gtgaaacgta tgcaggcggc aggcgcgcag
    57661 gcgtatctgg ttaacaccgg ctggaacggc actggcaaac gtatctcgat taaagatacc
    57721 cgcgccatta tcgacgccat cctcaacggt tcgctggata acgcagaaac cttcactctg
    57781 ccgatgttta acctggcgat cccaaccgaa ctgccgggcg tagacacgaa gatcctcgat
    57841 ccgcgtaaca cctacgcttc cccggaacag tggcaggaaa aagccgaaac cctggcgaaa
    57901 ctgtttatcg acaactttga taaatacacc gacacccctg cgggtgccgc gctggtagcg
    57961 gcaggtccta agctgtaatc agctgatatc tcattgaccc cttatccaaa cggataaggg
    58021 gtttttttat ctaattagct accaatatgt acacggtaca cttataatta tcaatagacc
    58081 gcaggatgca ttatgttcac ctttattgaa ctacaaggat ttagcaaacg ccgccagtta
    58141 ttgctacctg atgatgagtt ccgggctttt caggaactac tcattcaaga tcccactgcc
    58201 ggtgacatta tcgcaggcac aggcagtttt agaaaaattc gctggaaccg ttctggaatg
    58261 gggaaacggg gtggcactcg gataatttat tacaacgtaa cacgcaatgg ccgaatttat
    58321 ctggcgttga tttatcctaa aaatgaacag gatgatttaa cagaagaaca aagaaaggca
    58381 ttgaaactat tgtccgagaa gctgctttaa tgttgaagct ccacaaattc caccatggcc
    58441 gaaatgtcaa cggaggcatt gaaaacagat gaaagatgaa ttatttgctg atcttcttgc
    58501 cagcgcagaa gaaatggtac gcatcgaaaa gggtgaagaa acgccagaac cagagcacgt
    58561 ccatactttt agcgaaatcg atgtaaaagc gatccgtgaa gcaacgggtt tacgtcagca
    58621 agattttgct gtcgccgtag gggtaagtta tgacctggtc aaaagctggg aaaccaaacg
    58681 ccgtcagcct accggagcac cccgaaaatt gctcctgctt ttgcaagcga atcctttcat
    58741 tatcaatcaa ttgaaaacaa tctaaaaacg ggaggcacct tcacctcccg ttttatttac
    58801 ccttcttttg tcatgccctg cgcccgcgtt accggcactg gcagccaggc acgaatggaa
    58861 agcccgcccc gctcgctggt gccaagctcc aacatgccgt tatggttatc cacgatacgc
    58921 tgcacaatcg ccagccctaa tcccgtgccg ctaatagtgc gcgcactgtc gccacgaaca
    58981 aacggctgga acaggtgctt acgttgttcc ggcgcaatac ccggaccgtc atcttctacc
    59041 tggaaccagg cgcgattcgg ctccgtgccg ctgctgactt tgatccagcc attgccgtaa
    59101 cgggcggcgt tgaccaccat attcgccacc gcgcgtttga tcgacagcgg gtgcattttc
    59161 acctcaatgc tgccggggta aagcgcggtt tcaatttccc gctcatagcc actttcggcg
    59221 gcaatcacct caccgagcac ggcgttgaga tccgccattt ccatcggcat ctcctgcccg
    59281 gtgcgcaggt agtcgataaa ctgctcaatg atggcgttgc actcttcgat atctttattg
    59341 atcgattctg ccaggtagcc atcctgctcg ctcatcatct cggtcgccag acgaatacgc
    59401 gtcagcggcg tgcgcagatc atggcttacc cccgccatca gcagcgtacg gtcatccgcc
    59461 agttgcttaa caccagccgc catatggtta aaggcgcggg taacggaacg cacctccgaa
    59521 gcgccatatt cacgcagcgg cggcggaata atccctttac ccacctgcaa ggctgcgtgt
    59581 tcgagatcga ccaacggtcg gttctggata cgaataaaca gccacgcccc gcctatcgcc
    59641 aatagcataa tcgccaacgt atagcggaac agcggggaga aatcgccctg atgaatttcg
    59701 gttagcggca cgcgtaccca gatattgggc gacaaccagg ttttcagcca gacgacaggc
    59761 gaacttttgt tgacctcgac gcgcacttcc gtcgggccgc ccagttgctg cgccatctga
    59821 tggcttaaga attcatagtg ttgtgcccaa cgcagacctg cctcttcggc agcttcgttg
    59881 gaatagagag agatccccag ctcacggtag atctcccgac ggaaagcggg aggcacaacc
    59941 aactgcgtgc cgtcctccag ttgcagtttg tcggtcatca acatacgcac ttcgtacgcg
    60001 aggactttat taaactgctg gaggctcggc aaaatcgcga agttcagcac caccagataa
    60061 gtcgtcacca ggctgacgaa cagcaaggtg acgatgagca ataacgtgcg ggcaaatgaa
    60121 cttcgtggcg agaagcgcaa tcgcctcatg ctttagaacc gtccggtaca aagacgtagc
    60181 ccagacccca gacggtctga atgtaacgcg gatgcgccgg atcttcttcc accatgcggc
    60241 gtagacgcga aatctgcacg tcgatggagc gttccattgc ggagtattca cggccacggg
    60301 caaggttcat cagcttatcg cgggagagcg gctcacgcgg atggctgacc agtgccttca
    60361 gtaccgcaaa ctcaccgctg gtgagcggca tcggctcatc ttcgcggaac atttcacgcg
    60421 taccgaggtt aagtttgaac ttaccgaaag caattaccgc ctcttcctgt gacggcgcgc
    60481 ccggcagttc gttggcctga cgacgcagca ctgcacgaat gcgggccagc aattcacgcg
    60541 ggttaaacgg ttttggaatg tagtcgtcag cgccaatctc caggccaacg atacggtcca
    60601 cttcttcccc tttcgccgtc accataatga tcggcatcgg attgctctgg ctacgaagac
    60661 gtcggcaaat cgacaagcca tcttcaccag gtaacatcaa atccagtacc ataagatgga
    60721 aagattcacg agtcagcagg cgatccatct gttctgcatt agcgacgctt cgaacctgga
    60781 agccttgttc ggtgagataa cgttccagca gcgcgcgcag gcgcatgtcg tcatcaacca
    60841 ccagaatctt gtagttctct tgcattgttt gtactcccaa aggttcgcaa caatttgtaa
    60901 gcgtgtattc ttaaaaaagc tcacgttcgt caccagctaa atctggtatg aatttcagcc
    60961 taaattgtta caaagcatat taaacagcag cttaagtata caatttattc ggcgaaacat
    61021 tattgattct gttgatatga tcacgttata cccaatgtgc gcattatcaa acagacaaag
    61081 ggaatcaacg agatgaaaac gcccctggtt acccgggaag ggtatgaaaa actcaaacaa
    61141 gagcttaatt atctctggcg tgaagaacgc ccggaggtca caaagaaagt gacctgggcc
    61201 gcaagtttgg gcgaccgcag cgaaaatgct gactatcagt ataataaaaa gcgcctgcgt
    61261 gaaatcgacc gtcgcgtgcg ctatctcact aaatgcatgg aaaatctcaa aatcgtcgat
    61321 tactccccgc agcaggaagg caaagtcttt tttggcgcgt gggtggagat tgaaaacgac
    61381 gatggcgtga ctcaccgttt ccgtattgtc ggctacgatg aaatttttgg ccgtaaagat
    61441 tacatctcta tcgattcccc gatggcccgc gcattgctga aaaaagaagt cggcgatctg
    61501 gcggtggtga atacccctgc cggggaagcg agctggtatg ttaatgctat cgagtacgtg
    61561 aaaccgtaag gaagagtctt aacctcctgc cgatggctgg catttttgcc agccagtccg
    61621 tataactatc ccctgatttt tgatccgaaa agatgaactc aaaccatgat gaatgattcg
    61681 ttctgccgca ttattgcggg tgaaattcag gcgcgcccgg aacaggttga cgctgccgtt
    61741 cgcctgcttg acgaagggaa taccgtgccg tttatcgcac gttatcgtaa ggaaatcacc
    61801 ggcggtctgg atgacacgca gctgcgtaat ctggaaacgc gtctgagcta tctgcgcgag
    61861 ctggaagaga ggcgtcaggc gatcctcaaa tccatttccg agcaaggcaa actcaccgat
    61921 gatctggcga aggccatcaa cgccacctta agcaaaaccg aactcgaaga cctctaccta
    61981 ccctacaaac ctaaacgccg cacccgcggg caaatcgcca ttgaagcagg gcttgagccg
    62041 ttggctgacc tgctgtggag cgatccgtca cacacgccag aagtcgcggc tgcacaatat
    62101 gttgatgcag ataaaggcgt ggcagatacc aaagccgcgc tggacggcgc gcgctatatc
    62161 ctgatggaac ggtttgccga agatgccgcg ctgctggcaa aagtgcgtga ttatctgtgg
    62221 aagaacgcgc atttggtttc gacggtggtg agcggtaaag aagaggaagg ggcgaaattc
    62281 cgcgactatt tcgatcatca cgaaccgcta tccacggtgc cttctcaccg cgcgctggcg
    62341 atgttccgtg ggcgtaacga aggcatactt cagctttcgc tgaatgccga tccgcagttc
    62401 gaagagccgc cgaaagagag ctattgcgag caaatcatca tggatcacct tggcctgcgc
    62461 ctgaacaatg ccccggcgga tagctggcgc aaaggcgtgg tgagctggac gtggcgcatc
    62521 aaggtgctga tgcatctgga aaccgaactg atgggcaccg tgcgcgaacg cgcggaagat
    62581 gaagcaatca acgtctttgc ccgtaacctg cacgatctgc tgatggcggc cccagccgga
    62641 ctgcgtgcaa cgatgggcct cgatccgggt ctgcgtaccg gggtaaaagt agcggtggtc
    62701 gatgcaactg gcaaactggt ggcgaccgat accatttatc cacataccgg acaagccgca
    62761 aaagcagcga tgaccgttgc tgccctgtgt gaaaagcata acgttgaact ggtggcgatc
    62821 ggcaacggta cagcttcccg cgaaaccgag cgtttctatc tcgacgtgca gaagcagttc
    62881 ccgaaagtga ccgcgcagaa agtgatcgtc agcgaagctg gcgcgtcggt ttactcggct
    62941 tccgagctgg cagcacagga gttcccggat ctcgacgttt cgctgcgtgg cgcggtgtct
    63001 atagcccgcc gtttgcagga tccgctggcg gagctggtga aaatcgatcc gaaatctatc
    63061 ggcgtaggtc agtatcagca tgacgtcagc cagacgcaac tggcccgcaa actggacgca
    63121 atagtagaag actgcgtaaa cgccgttggc gtcgatctca acaccgcttc tgttccgctg
    63181 ttaactcgcg tggcgggcct gacgcgcatg atggcgcaaa acatcgttgc ctggcgcgat
    63241 gagaacggtc agttccagaa ccgtcagcaa ctgctgaaag tgagccgtct ggggccgaaa
    63301 gccttcgagc agtgcgcggg cttcttgcgc attaaccatg gtgataaccc gctggacgcg
    63361 tctaccgtcc acccggaagc ctatccggtg gtggaacgca ttctggcagc aacacagcag
    63421 gcactgaaag atctgatggg taacagcagc gagttgcgta acctgaaagc gtctgacttt
    63481 actgatgaaa aattcggtgt accgacggta actgacatca tcaaagagct ggaaaaaccg
    63541 ggtcgcgatc cgcgtccgga atttaaaacc gctcagtttg ccgatggcgt cgaaacgatg
    63601 aacgacctgc aaccgggtat gatcctcgaa ggtgcggtga ccaacgtcac caactttggc
    63661 gcgtttgtcg atattggcgt tcatcaggac ggcctggttc acatctcttc attgtcgaac
    63721 aagtttgtgg aagatccgca taccgtggtg aaagcgggcg atattgtgaa ggtgaaagtg
    63781 ctggaagtgg atcttcagcg caaacgtatc gccctgacta tgcgtctgga cgagcagcct
    63841 ggcgaaacca acgctcgtcg cggcggcggt aatgaacgtc cgcagaacaa ccgcccggca
    63901 gccaaaccgc gtgggcgtga agcgcagcct gccggtaata gcgcgatgat ggatgcgctg
    63961 gcggcggcaa tgggcaaaaa acgttaaacg cccggactgg cccctaaacc tgcgcgatta
    64021 ggcgttcacg ccacatctgg caacgaacgc cttgcctgac ataaaagtgc cggagaatat
    64081 ctccggcctt tttattccac aagcaaactc ataatatatc ccgttaatat ttatcatttc
    64141 attaacaact gaaaccttaa ttaaacatta accagcccta ataattcgtc atgcgaatta
    64201 tattttcgct gcgatataac cttgagccat atcaacattg agtcagatta ttattcaaac
    64261 caacattcac acacatttta agtattgctg atagaaacca ttctcattat cattgtactg
    64321 ttgattattt aatctctcct tcgttggcaa atcatctggt ctcatgtcgc tgtcaaacgc
    64381 cccatgaggt agttatccag ttaatgagaa acaagtaggc acctatgcaa tacactccag
    64441 atactgcgtg gaaaatcact ggcttttccc gtgaaatcag cccggcatat cgccaaaaac
    64501 tgctttctct tggcatgtta cctggctcct cttttaatgt ggtgcgcgtc gctccactcg
    64561 gcgaccccat tcatatcgaa acccgtcgtg tgagcctggt attacgcaaa aaagatctgg
    64621 ccttattaga agtggaagcg gtttcctgtt aatacggtga taacaacaat gaaaaaatta
    64681 accattggct taattggtaa tccaaattct ggcaagacaa ccttatttaa ccagctcact
    64741 ggcgcacgtc agcgtgtagg taactgggct ggcgttaccg tcgaacgtaa agaagggcaa
    64801 ttctccacca ccgatcatca ggtcaccctg gtggacctgc ctggcaccta ttctctgacc
    64861 accatctcat cgcagacctc gctcgatgag caaatcgcct gtcactatat tctgagtggc
    64921 gacgccgacc tgctgattaa cgtggtggat gcgtctaacc ttgagcgtaa cctgtacctg
    64981 acgctgcaac tgctggaact cggcattccc tgcatcgtgg cgctgaacat gctcgacatt
    65041 gccgagaagc aaaatattcg tattgaaatt gatgctctgt cggcgcgtct tggctgcccg
    65101 gtgatcccgc tggtttcaac ccgtggtcgc ggtattgaag cgctcaagct ggcgattgat
    65161 cgctataaag ctaacgagaa tgtggaactg gtgcattacg cacagccgct actcaacgaa
    65221 gcagattcac tggcaaaagt gatgccttcc gacatcccgc tgaaacaacg tcgctggctg
    65281 ggtctgcaaa tgctggaagg cgatatctac agccgcgcct acgccggtga agcgtcgcag
    65341 catcttgatg ccgccctcgc ccgtctgcgt aatgagatgg acgatccggc gctgcacatt
    65401 gccgatgcgc gttaccagtg cattgctgct atctgtgatg tggtaagcaa caccttgacg
    65461 gcagaaccca gccgtttcac cactgcggta gataaaatcg tgctcaaccg tttcctcggt
    65521 ctgccgattt tcctctttgt gatgtacctg atgttcctgc tggctatcaa catcggcggg
    65581 gcgttacagc cgctgtttga cgtcggctcc gtggcgctat ttgtgcatgg tattcagtgg
    65641 attggctaca cgctccactt cccggactgg ctgactatct tcctcgccca gggcctgggc
    65701 ggcggtatta acaccgtgct gccactggtg ccgcagatcg gcatgatgta cctgttcctc
    65761 tccttcctcg aggactccgg atatatggcg cgtgcggcgt ttgtgatgga ccgtctgatg
    65821 caggcgctgg gcttgccggg gaaatccttt gtaccgctga tcgttggttt cggttgtaac
    65881 gtaccgtcgg taatgggcgc acgtacgctt gatgcaccgc gtgaacgtct gatgaccatc
    65941 atgatggcac cgtttatgtc ctgcggcgcg cgtctggcta tcttcgccgt atttgcggct
    66001 gctttcttcg ggcagaacgg tgcgctggcg gtcttctcgc tgtatatgct gggtatcgtg
    66061 atggcggtgc tgactggcct gatgctcaag tacaccatca tgcgcggtga agcgacgccg
    66121 tttgtcatgg agttgccggt ctatcatgta ccacacgtta aaagcctgat tatccagacc
    66181 tggcagcgtc tgaaaggctt cgttctgcgt gctggtaaag tgattatcat cgtcagcatt
    66241 ttcctgagcg ctttcaacag cttctcgctg agcgggaaaa tcgtcgataa catcaacgac
    66301 tcggcgctgg cgtccgtcag ccgggtgatc accccggtct tcaagccaat tggcgtgcat
    66361 gaagataact ggcaggcaac ggttggcctg tttacaggtg ccatggcgaa agaagtggta
    66421 gtgggtacgc tcaacaccct ctacaccgca gaaaatattc aggacgaaga gttcaatccg
    66481 gcagagttta acctcggtga agagctgttc agtgcgatag atgaaacctg gcagagcctg
    66541 aaagacacct tcagccttag cgtactgatg aaccccattg aagccagcaa aggcgacggc
    66601 gaaatgggta ccggggcgat gggcgtgatg gatcagaaat tcggtagcgc agctgccgca
    66661 tacagctacc tgattttcgt cctgctgtat gtaccgtgca tctcggtgat gggggctatc
    66721 gcccgtgaat caagccgtgg ctggatgggc ttctccatcc tgtgggggct gaatatcgct
    66781 tactcactgg caacattgtt ctatcaggtc gccagctaca gtcagcaccc aacttacagc
    66841 ctggtgtgca ttctggcggt tatcctgttt aacatcgtgg ttatcggtct gctgcgccgc
    66901 gcgcgtagcc gggtggatat cgaactgctg gcaacccgca agtcggtaag cagttgctgc
    66961 gcagccagca ccaccggtga ttgccattaa tggcttcact tattcaggtg cgtgatttgc
    67021 tggcgttacg gggccgtatg gaagcggccc agataagcca gacattgaac actccgcagc
    67081 caatgattaa cgccatgctg caacagctgg aaagcatggg caaagccgtg cggattcagg
    67141 aagaacctga cggctgcctc tctggcagtt gtaaaagctg cccggaaggc aaagcctgtc
    67201 tgcgcgagtg gtgggcgctg cgttaaccat actccatcgc ctgttttgaa aagcccggta
    67261 tgcgtttgca tccgggcttt tttgcgtgcg gctttccata aaaatgtaac ccttgcagca
    67321 cggcgcaagt tcctctgaaa gcgtctcgca gggataaaaa ctcgctaatg cacaggtgtt
    67381 gagtggcgcg tagagtcgcg gcattcaaac aacaggtgaa ggaacgccat gagcaaaaag
    67441 cagagttcca ccccacacga tgcgctgttc aaactctttt tacgccaacc ggagacggca
    67501 cgcgattttc ttgcgtttca tttaccggca cccattcacg cgctctgtga tatgaaaacc
    67561 ctcaagctgg agtcgagcag ctttattgat gacgatctgc gtgaaagcta ttccgatgtg
    67621 ctgtggtcgg tgaaaacgga acaaggacca ggatacatct attgtctgat tgaacatcaa
    67681 agcacctcaa acaaactgat cgcatttcgc atgatgcgtt acgctattgc cgcaatgcaa
    67741 aatcacctgg atgccggata caaaacgttg ccgatggtgg tgccattgtt gttttaccac
    67801 ggcattgaaa gcccctatcc ctattcactg tgttggctgg attgtttcgc cgatcccaaa
    67861 ctggcaaggc agctttatgc ctctgcattt ccgctgattg atgtcactgt catgcctgat
    67921 gatgaaatca tgcagcaccg gcgcatggcg ctgctggagc taattcaaaa acatatacgt
    67981 caacgcgatc tgatggggct ggtagagcaa atggcctgct tattaagtag tggatacgct
    68041 aatgacagac aaatcaaagg gctgtttaat tacatcttgc aaaccggcga tgcggtacgt
    68101 tttaacgatt ttatcgacgg cgttgccgaa cattcaccga aacacaagga gagcttaatg
    68161 actattgcgg aaagattgcg gcaagagggg gaacaatcca aagccttgca tatagcaaaa
    68221 ataatgcttg aatccggagt ccctcttgcg gacatcatgc gttttaccgg ggtgtcagaa
    68281 gaagagttgg ctgcggcgag tcagtaaagt tctgtctcgc catttcaaaa gccacctaca
    68341 ccctctgctt caacgccacc agcatgcgac aaaactcggc cggatgcgaa ataaacggcg
    68401 catgggccgc tttggcgaag atgtatgatt cgctgtgagg ccaaagttta tccagcatcg
    68461 gcaccacttt gcgcggcacc agaccgtcga gatagccata caatcgcaaa aacggcatgg
    68521 acacgttttg cagcggctga cggagatcga ccgttttcag gatttccagc ccgccattaa
    68581 gcacgtcaac ctccggcatc ggtaacgcca gaacggtttt cttcagcgcc cgcgcatcct
    68641 ggcgcgccgt ttcagtaccc atggtttgta acgccaggaa ccgctccact gtacgctgaa
    68701 aatcatcact gagttgctgc tgaaatcccg ccagcacgtc cggttttatc cccggccact
    68761 cgtcacgagc actaaaacaa ggtgacgacg ccacggtgac cagcgcctga acacgctcgg
    68821 gatgggttaa cgcaatctgg cttgccacca gcccgcccag actccagcct aaccaaatgg
    68881 ctttatcagg tgcctgtcgc agcacggctt cggccatatc agcaagtgac atcgcaccaa
    68941 atccccggct acgcccgaag ccgggcaggt caacaaggtg cagcgtaaaa tgcgagctca
    69001 gttcctcgtc aatgcaacgc cacacttcgg cattcagtcc ccatccgtgc agcagcacaa
    69061 gatgaacatt cccctgacct ttggtctgcc accagatgtt attcatccgc tattgttctc
    69121 ttttgactta caaggatgaa catatgctaa cagtaccggg attatgctgg ctatgccgaa
    69181 tgccgctggc gttaggtcat tgggggattt gttcggtctg ctcactcgcc gcccgtacag
    69241 ataaaacgtt atgcccacaa tgtggattac ccgccacaca ctcacatctt ccctgcggtc
    69301 gctgcctgca aaaaccaccg ccctggcaaa gactggtcac ggttgccgac tatgcgccac
    69361 cgttaagccc gctaatccac cagcttaagt tttcccggcg cagcgaaatc gccagcgccc
    69421 tgtcacgtct gttactattg gaggtcttac acgctcgtcg caccaccggg ttgcaattgc
    69481 cggatcgcat catcagcgtt ccgttatggc agcggcgtca ctggcgtcgg ggatttaatc
    69541 agagcgattt gctgtgtcag ccgttatcac gctggttgca ctgccgatgg gatagcgaag
    69601 ccgtcacacg aacacgggcc actgcgaccc agcattttct cagtgcccgg ctgcgcaagc
    69661 gcaacctgaa aaatgccttt cgccttgaat tgcctgtgca aggtcgccat atggtgattg
    69721 tggatgatgt cgttaccacc ggaagtaccg tcgcagagat tgcgcagttg cttttacgca
    69781 atggtgcggc gactgtccag gtctggtgcc tttgtcgaac cttgtagagc ctcgatgatg
    69841 ggcgtattat aaccaactaa aatagtcaac tattaggcca ttactatgat ccgtatttcc
    69901 gatgctgcac aagcgcactt tgccaaactg ctggcaaatc aggaagaagg gacacaaatc
    69961 cgcgtatttg tgattaaccc tggcacgcct aacgctgaat gtggcgtttc ttattgtccg
    70021 ccggatgctg tggaagccac cgacactgcc ctgaaatttg acctgctgac cgcgtatgtt
    70081 gatgagttaa gcgctcctta cctggaagat gcagagatcg actttgttac cgaccagttg
    70141 ggttcccagt taacgttgaa agcgcctaac gccaaaatgc gtaaagtcgc agacgatgcg
    70201 ccgctgatgg agcgcgttga gtatatgctg cagtcgcaga tcaacccgca actggctggt
    70261 cacggtggtc gcgtttcgct gatggaaatc accgaagacg gttacgccat tctgcaattt
    70321 ggtggcggct gtaacggttg ttccatggtc gatgtgacgc tgaaagaagg gatcgagaag
    70381 cagctgctaa acgaattccc ggagctgaaa ggtgtgcgcg atctcaccga acaccagcgc
    70441 ggcgaacact cctactacta agttatcccc tcttttgtag attgcccgat gcgacgctaa
    70501 agcgtcattt cgggcctacg ttgaacatgt gccagacgtt ggcgctgcat tgctttggca
    70561 aatccctccc ttcgtttccc cgcgataata tgaccaacct ctcataattt aaatttaccc
    70621 cgctctggtg attctcaaac gccagatgtt acccgtatca ttcacgtggg taccaaacat
    70681 actcctgaca tctgactaca ataattagtt ttagtgggta tcagtcgtgg tgccgcaata
    70741 tctctgttcc cgattgggat aattagagtt tgtcgtcaga aaattgacgt tacccataac
    70801 aaatgaaagg ccaggtaaat catgccatta gtcattgttg ctatcggtgt aatcttgttg
    70861 ttgctcctga tgatccgctt caaaatgaac ggtttcatcg ctctcgtcct cgtggcgctt
    70921 gctgttggat taatgcaagg gatgccgctg gataaagtta ttggctccat caaagccggt
    70981 gtcggcggaa cgctcggtag ccttgccctg atcatgggtt ttggcgcgat gctgggcaaa
    71041 atgctggcag actgcggtgg cgcacaacgt atcgccacca cgctgattgc taaatttggt
    71101 aaaaaacaca tccagtgggc ggtggtactg accggtttta ccgttggttt tgccctgttc
    71161 tatgaagtgg gtttcgtgct gatgctgccg ctggtgttta ccatcgcggc ttccgcgaat
    71221 attccactgc tgtatgttgg tgtaccaatg gcggctgcac tgtctgtgac ccacggcttc
    71281 ctgccaccgc atccgggtcc gactgcgatt gccaccattt tcaatgccga tatgggtaaa
    71341 actctgctgt acgggactat tctggcaatc ccgaccgtga ttctcgccgg tccggtttac
    71401 gctcgcgtgt tgaaaggtat cgataagccg atcccggaag gtctgtacag cgcgaaaacc
    71461 ttcagcgaag aagagatgcc gagctttggc gtcagcgtct ggacctctct ggtgccggta
    71521 gtgctgatgg cgatgcgtgc gattgccgaa atgatcctgc cgaaaggtca cgctttcctg
    71581 ccggtagcgg agttcctcgg tgacccggta atggctacgc tgattgccgt actgattgcg
    71641 atgttcacct ttggtctgaa ccgtggtcgt tcaatggatc agattaacga cacgctggtt
    71701 tcttccatca aaatcattgc gatgatgctg ttgattatcg gtggtggcgg tgcgttcaag
    71761 caggtgctgg tagacagcgg cgtggacaaa tacattgctt ccatgatgca cgaaaccaac
    71821 atttctccgc tgctgatggc ctggtcgatt gctgccgtac tgcgtatcgc gctgggttcc
    71881 gcaaccgttg cggcaatcac tgcgggtggt atcgcggcac cgctgattgc aacgacgggt
    71941 gttagcccgg agctgatggt tattgcggtt ggttccggta gtgtgatttt ctctcacgtg
    72001 aacgatccgg gcttctggtt gttcaaagag tactttaacc tgactatcgg cgagaccatc
    72061 aaatcctggt cgatgctgga aaccattatc tcggtgtgcg gtctggtagg ctgtctgctg
    72121 ctgaatatgg tgatttgata acactgactg ccggatgcat cgcatccggc atcaaccgca
    72181 ctctacttct tcttcgctgc cgctctgcgc cgtctgtcca aatccttcag caacttgttc
    72241 acgccatcat cggcaaacat cgcctcaagc gttgcggaga gcttgcgccg ccagttttta
    72301 tactggtaac tggtgccggg aatatttacc gggtccgcca tatccagcca gtcttctggc
    72361 tgtagtccta acagggcgct gttgctatcg gcgatgtagc gctgtaagcc acgattcagc
    72421 gtcggcgtca tcgacatcaa cgatgcctta tgcccggcac gttttggcag acaaccatat
    72481 ttatgcagag catccagtag tccttgcttc gccagttcgc gatcctgata cagaccgcgc
    72541 agtaccactt catccggata caaccccagc gttttgccca gcgttagatc cccgctctcc
    72601 cagtagccgc gtagcgtagg caggtcgtgt gtcgccgcaa ccgccatcga ctgctctggg
    72661 tacgctttcg gtgagcggaa tgtcttctcg tggtcgtttt cgaaatagag cactttgtag
    72721 gagtacacac cgctgctgcg cagcttaccg acaatctcta ccggtacggt accgagatct
    72781 tcaccaatca ccatgcagcg atgacgttta ctttcgagtg ccagaatcga gagcagatca
    72841 tccactggat agtgaacata tgccccctga tccgccgtct cgccatacgg tatccaccac
    72901 aaacgcagca tcgacatcac atggtcaatt cgtaatgcgc cgcagttttg catattggca
    72961 cgcaacagct cgataaacgg ttcataggca cgcgcggtga taatatgcgg gtccattggc
    73021 ggcaatcccc agttctgccc caacgggccg aggatatccg gcggcgcgcc aaccgacgct
    73081 ttcaggcaat acagttcacg gtcgcaccag gtttctgccc caccttctgc tacaccaacg
    73141 gccagatcac gatacaagcc aatcggcatt tcatagtcct ggcttatctc ccagcaggcg
    73201 gcaaactggc tgtaagccag ccactgcaac cagagataaa aatcgacgtc atcacgatgt
    73261 tcttcgcaga actggcgcac ttctggtgaa tccacgttct gatacatctc tggccatgca
    73321 ggccagcccc agcgcatttc gtcctctttc acttgctggg catgtagtgc gtcaaacgcc
    73381 gcctgccaga acaggctgtc gccttgctct gcaacaaact ggcgaaacgc ggccacctgt
    73441 tcatcatcac gttgcgcgaa acctttccac gccattcgta atgctgtcat ttttagggcg
    73501 gtaaccgtgg agtaatcgac ccagtcggca tcccgcgcct gttgcagcgt ctgttgcgtg
    73561 gtcggcaact tccaccaggc cagagcctct tcgctaagat ggaaatcttc aacggcgtta
    73621 acgtcgatat aaatcacatt cagccaacgg cgagaagacg ggctatacgg gctggcactt
    73681 tccggatttg ccggatagag cgcatgaatc gggttcagac cgatgaacga cccgccacgt
    73741 tttgccacat ccaccagcat cgctttgaga tcgccaaaat ccccaatacc ccagtttttt
    73801 tccgatcgca gcgtataaag ctgaacgcag gccccccaca gcttttgttt gttcagcaac
    73861 gcctgcggtt cgtaacagcg tttcggtgcc acaatcaccc ggcaatgagc acgctggtcg
    73921 tcctgggtga gtgtcagcgt gtgataacct tccggcagct tcgtcggtag attgaacgct
    73981 ttgccccccg ttacatggcc tttgtactgc gttccttctt cggtggtcag cagccagcta
    74041 tattcgccgc tgccctccac caccatcggc atttttttgc cgctggtata aaccatgaca
    74101 ttcgggactg gcgttaccgc cactttcgtg gcggtacgtt gatgcatcgc gtcaagcaaa
    74161 cgccgtttgg tttcggcgct aatcgactgc ggtttaccgt gggcattgat gtaattgggg
    74221 ctgatccccg ccgccagcgc ggcattatcc agacgtttgc tttccatcga gcttccttag
    74281 cgttttgcct gccagatacg agcctgataa tcgcgaatag agcgatccga gctaaacata
    74341 ccgcagcggg cggtattgag gatcgccgcg cgggtccagg cctcctggtc gcggtacagc
    74401 acatccacct gcttttgtgc ctctacatag gctgcgaaat ccgccatcac cagatacgga
    74461 tcgccgccct gtttgccgat actgtgcagc atctggtcga aggcatgctt atcgccgtcg
    74521 ctgtatttgc cgctttccag ctctttcaat actgcgtcca gcaccttatc tttcttccgc
    74581 catttcaccg ggtcgtagcc tttggccaga attgccttca cttgttccac ggtatgacca
    74641 aaaataaaga tattttcttc accaactttc tcggcgattt caacgttcgc accatccagc
    74701 gtaccgacag taagcgcacc attgagcgcc agtttcatat tgccggtacc ggaagcttct
    74761 ttacctgccg tcgaaatttg ttcggagata tccgccgccg ggatcagttt ttccgccgcc
    74821 gaaacgcaat aatccggcag gaacaccacc ttcaatttgt cgccaacctg cggatcgttg
    74881 ttgatcacgt cagccacttt gttgatcgca aagataatgt ttttcgccag gtagtagccc
    74941 ggtgccgctt tcgcgccaaa gaggaagacg cgcggtacgc gatcagcctg cgggttttca
    75001 cgaatttctt tgtacaacgc cagaatatgc agcagattca ggtgctggcg tttgtactca
    75061 tgcagacgtt tgatctgaat atcgaaaatc gcctgtggat tgatctcaat accggtacga
    75121 actttcacaa actctgccag acggacttta ttcgcctgct tgatctcgcg atattgctga
    75181 cggaatttcg catcatcagc gaatttttcc agattgatca gttgatcgag atcgttagcc
    75241 cactcttttt tcagtgattt atccaacaga gccgccagtg ccgggttgca ctgtttgatc
    75301 cagcgacgtg gggtaatacc gttggtgacg ttatggaatt tgttcggcca tagctggtga
    75361 tattccggga acagatcttt caccaccaga tccgagtgca gcgccgcaac accgttcacc
    75421 gcgaaaccgc caaccacaca caggttcgcc atatgcactt gtttgtcgtg taccaccgcc
    75481 agtttggccc acactttttc atcgcccggc cagttttttt ccaccagcgt tttaaagcgt
    75541 gtattaattt cgttaataat ctgcatgtgg cgcggcagta agcctttcac cagtttcaca
    75601 tcccagcgtt ccagcgcttc tggcatcagg gtatggttgg tgtaagcgaa agttttactg
    75661 gtgatagccc aggcgtcatc ccagctcatc tggtgctcat cgatcagcac gcgcagcagt
    75721 tctggaatcg cgattgttgg gtgggtatcg ttcagctgaa taacttcgta atccgccagt
    75781 tcgtgcagtt tacgccccgc cagatgatgg cgacgcaaaa tatccgctac cgaacaggca
    75841 cactggaagt attgctgcat caggcgcagc tttttaccgg cagtatggtt gtcgtttgga
    75901 tagagaactt tggtcagttt ttccgcgttg atgccctgct gttcggcgcg caggaaatca
    75961 ccgtcgttaa atttagtcag atcaaacgga tgcgcatgcg tcgcctgcca cagacgcagt
    76021 ggctgtgcca cgccattacg atagccgaca acggggagat cccacgcttg accggtaatg
    76081 gtaaattccg gctcccagcg tccgtctttc gtcactttac cgccaattcc tacctgcaca
    76141 tccagtgctt cattgtggcg gaaccacggg tagttactgc gatgccagtc atccggcgct
    76201 tcaacctgtt tgccatcgac aaatgactgg cggaacaagc catattgata attaaggccg
    76261 tagccagtag ctgactgccc gacagttgcc attgagtcga ggaagcacgc cgccagacgt
    76321 cccagaccac cgttacccag cgccgggtcg atctcttctt ccagcaggtc agtcaggttg
    76381 atgtcataag ccttcaacga atcctgtaca tcctgatacc agccgagatt caacaggttg
    76441 ttgcccgtca ggcgaccaat caaaaactcc attgagatgt agttaacatg tcgctgattc
    76501 gccaccggtt tggcaaatgg ctgagcacgc agcatttcgg ccagtgcttc gctcactgcc
    76561 agccaccact gacgaggggt catttcagcc gcagaattta agccataacg ctgccactga
    76621 cgtgaaagcg cttcctgaaa ttgcttatcg ttaaaaatag gttgtgacat aggagttcca
    76681 cttttcttag attttcaaca caacgttatc gctagtttgc caggctcgat gttgaccttc
    76741 ctcatcctgc gggggattag gcagggagga gttgcgggga tgagcaagga aatgtgatct
    76801 cgaccactta aagttagtgc aaaccacagg attagcagca aatcaatgcc atacagcgca
    76861 gaaaatctgt atctaagtgc aaaaaatggc cgttgcgtat tttcaaaaag cggaaggtaa
    76921 ctctataaat taagtaaagg agtgaaacag tttcataagt aaaatatcaa gtgtgctcca
    76981 tctcattctt aatagattta ttaagatcat ctttttagat ggcactttca tcagggataa
    77041 agaagaaacc cttgcttaaa tgaaactgat gaacataagg aaaataccgt acaacccagc
    77101 attcacgctg gatcagcgtc gttttaggtg agtcattaat aaagatttgg aattgtgaca
    77161 gagtgcaaat tcagacacat aaaaaaacgt catcgcttgc attagaaagg tttctggccg
    77221 accttataac cattaattac gaagcgcaaa aaaaataata tttcctcata ttccacagtg
    77281 aagtgattaa ctatgctgat tccgtcaaaa ttaagtcgtc cggttcgact cgaccatacc
    77341 gtggttcgtg agcgcctgct ggctaaactt tccggcgcga acaacttccg gctggcgctg
    77401 atcacaagtc ctgcgggcta cggaaagacc acgctcattt cccagtgggc ggcaggcaaa
    77461 aacgatatcg gctggtactc gctggatgaa ggtgataacc agcaagagcg tttcgccagc
    77521 tatctcattg ccgccgtgca gcaggcaacc aacggtcact gtgcgatatg tgagacaatg
    77581 gcgcaaaaac ggcaatatgc cagcctgacg tcactcttcg cccagctttt cattgagctg
    77641 gcggaatggc atagcccact ttatctggtc atcgatgact atcatctgat cactaatcca
    77701 gtgatccacg agtcaatgcg cttctttatt cgccatcaac cagaaaatct caccctggtg
    77761 gtgttgtcac gcaaccttcc gcaactgggc attgccaatc tgcgtgttcg tgaccaactg
    77821 ctggaaattg gcagtcagca actggcattt acccatcagg aagcgaagca gttttttgat
    77881 tgccgtctgt catcgccgat tgaagccgca gaaagcagtc ggatttgtga tgacgtttcc
    77941 ggttgggcga cggcactgca gctaatcgcc ctctccgccc ggcagaatac ccactcagcc
    78001 cataagtcgg cacgccgcct ggcgggaatc aatgccagcc atctttcgga ttatctggtc
    78061 gatgaggttt tggataacgt cgatctcgca acgcgccatt ttctgttgaa aagcgccatt
    78121 ttgcgctcaa tgaacgacgc actcatcaac cgtgtgaccg gcgaagaaaa cgggcaaatg
    78181 cgtctcgaag agattgagcg ccaggggctg tttttacagc gaatggatga taccggcgag
    78241 tggttctgct atcacccgct gtttggtaac ttcctgcgtc agcgctgcca gtgggaactg
    78301 gcggcggagc tgccggaaat ccaccgtgcc gccgcagaaa gctggatggc ccagggattc
    78361 cccagcgaag cgattcatca tgcgctggcg gcaggcgatg cgctgatgct gcgcgatatt
    78421 ctgcttaatc acgcctggag tctgttcaac catagcgaac tgtcgctgct ggaagagtcg
    78481 cttaaggccc tgccgtggga cagtttgctg gaaaatccgc agttagtgtt attgcaggcg
    78541 tggctgatgc aaagccaaca tcgctacggc gaagttaaca ccctgctagc ccgtgctgaa
    78601 catgaaatca aggacatcag agaaggcacc atgcacgcag aatttaacgc tctgcgcgcc
    78661 caggtggcga ttaacgatgg taacccggat gaagcggaac ggctggcaaa actggcactg
    78721 gaagagctgc cgccgggctg gttctatagc cgcattgtag caacctcagt gctgggtgaa
    78781 gtattgcact gcaaaggcga attgacccgc tcactggcgt taatgcagca aaccgaacag
    78841 atggcacgcc agcacgatgt ctggcactac gccttatgga gtttaatcca gcaaagtgaa
    78901 attctgtttg ctcagggatt cctgcaaacc gcgtgggaaa cgcaggaaaa agcgttccag
    78961 ctgatcaacg agcagcatct ggaacagctg ccaatgcatg agtttctggt gcgcattcgt
    79021 gcgcagctgt tatgggcctg ggcacggctg gatgaagccg aagcgtcagc acgtagcggg
    79081 attgaagtct tgtcgtctta tcagccacag caacagcttc agtgcctggc aatgttgatt
    79141 caatgctcgc tggcccgtgg tgatttagat aacgcccgta gccagcttaa ccgtctggaa
    79201 aacctgctgg ggaatggcaa atatcacagc gactggatct ctaacgccaa caaagtccgg
    79261 gtgatttact ggcaaatgac cggcgataaa gccgccgctg ccaactggtt gcgtcatacg
    79321 gctaaaccgg agtttgcgaa caaccacttc ctgcaaggtc aatggcgcaa cattgcccgt
    79381 gcacaaatct tgctgggcga gtttgaaccg gcagaaattg ttctcgaaga actcaatgaa
    79441 aatgcccgca gtctgcggtt gatgagcgat ctcaaccgta acctgttgct gcttaatcaa
    79501 ctgtactggc aggccggacg taaaagtgac gcccagcgtg tgttgctgga cgcattaaaa
    79561 ctggcgaatc gcaccggatt tatcagtcat tttgtcatcg aaggcgaagc gatggcgcaa
    79621 caactgcgcc agttgattca gcttaatacg ctgccggaac tggaacagca tcgtgcgcag
    79681 cgtattctgc gagaaatcaa tcaacatcat cggcataaat tcgcccattt cgatgagaat
    79741 ttcgttgaac gtctgctaaa tcatccggaa gtgccagaac tgatccgcac cagcccgctg
    79801 acccaacgtg aatggcaggt cctggggctg atttactccg gttacagcaa cgagcaaatt
    79861 gcaggcgagc tggaagtggc ggcaaccacc atcaaaacgc atatccgcaa tctgtaccaa
    79921 aaactcggtg tcgcccaccg tcaggccgcg gtacaacacg cgcagaaact gctgaagatg
    79981 atgggatatg gggtatgagt ttagccggat aatacgtcgg atccggctta catctttata
    80041 tcattcaatg ctcacccgcg ttacgccatc agcctccacc agaccaaacc gcactggcaa
    80101 gaaacgctcc accaccgcaa tattggtcag cagatggcat gagggatggg cgaccgtaaa
    80161 ttctcccgcg cccgccagcg ccatcggtag caccagttgg tcggcgagat attcccccac
    80221 cgccgccggg cttgccaggt agcgtttcac ctctttcacc aactgtgccg cgaccacctc
    80281 agcgctgacg cgcttttcac cgacgacaaa aaagcgttcg gtgatatttt cactttcgac
    80341 ttcaagcgag acggtattac ccggcccctg gtcacgcggc aggttatgaa tattctgctc
    80401 atgcagggaa aaactcccca ccagcgtagc gatttcacgc tcagcaacat gcctcggcac
    80461 gccagctaat agcacctctc cacgcatctg cacaatgttc ccgcgctcgc caagttgcaa
    80521 ggtgttaaac aatgccacgg gcgagacttc cgttgccacc accccgcctc cggcaggata
    80581 aaaaccgtgg cgtattagtg tggtttgctg atgaatgccc attttcgcca gcagcggctc
    80641 cagcacccgg cggataaaat ccgcaggcgg ggccgacggg ttatcggtgc ctccgctcac
    80701 ttcaacacgc gaaggtccat cggcaaacca cagcgcgggc agcaccgttt gcagcaccag
    80761 cgtacaactt ccggcgctac cgatagcaaa gcggtaatcg ccgccgcgca cggtgccggg
    80821 ccggaagagc agacgctgcg atcccagctc cgcaccttcc accgttgccc tacaaatttc
    80881 cgcagccgct tttaccgcgg tcagatgctg gcgcaacagt cccggttttg cccgcccggc
    80941 acgaatgccg gtgatggtaa atggcaggcc ggttatcatc gacaggctca gcgccgagcg
    81001 caggatctgc ccgccgcctt cgccctgtgc gccatccagc gcaatcatcc ttttcatcat
    81061 ttatcctttt acgcacacca cctgacgcag ggtatagata acttccacca gatcgctttg
    81121 tgccgccatc accgcatcaa tatctttata cgccatcggg atttcgtcga tcacttcggc
    81181 atctttacgg cattccacat gcgcggtggc acgaatttgg tcttccacac tgaacagttt
    81241 tttcgcttta gtacggctca ttacccgccc ggcaccgtgg ctgcacgaac agaacgactc
    81301 ttcatttccc agcccacgga caataaagct tttcgccccc atcgaaccgg gaataattcc
    81361 atattgacca gcacgagcag acaccgcgcc tttacgcgtc acatagatct cttcgctaaa
    81421 gtgctgttct ttttgcacat agttgtggtg acagttgatc tcttccatcg ccagcgtttg
    81481 tggctgtctt accgttttct gcgtaacgct ctgcaacgcc gttaccacgt tttccatcat
    81541 cgcatcgcgg ttaaggctgg caaaaagctg cgcccaggcc accgctttca ggtaatcgtc
    81601 aaaatattcc gtaccttcca taaagtacgc caggtcacgc gatggcaacg tttcaagtgt
    81661 ttcctgcatc tctttttgcg ccagatcgat aaagtaagtc ccgatggcat taccaattcc
    81721 gcgtgaaccg gagtgcagca taatccacac ctgatccgac tcatcaaggc aaatttcaat
    81781 aaagtggtta cccgttccca gcgttcccag gtgtttatag ttattggtat taaggaaacg
    81841 cggatatttt tgcgttaacc actgataacc ggcttcaagc tcagcccatt tcgcatcgac
    81901 gttaacaggc ggtttttccc atgcgccttt atcacgttta caacggcctg tggtacgccc
    81961 atgcggcacg gcagcttcaa tcgcctgacg cagctccgcc aggttttcag gcaggtcagc
    82021 cgccgttaac gcggtacgca gcgcgttcat tccacaaccg atatctaccc ccaccgccgc
    82081 cggaataatc gctcctttgg ttgggatcac gctaccaatg gtggaacctt tacccagatg
    82141 tacatcaggc attaccgcaa tatgtttgaa aataaacggc atcttcgccg tatttatcag
    82201 ttgctgacgc gcatcgtctt ctaccggtac gcctttggtc cacattttta ccgggacatt
    82261 ttcagtggtc agtaattcgt aattcatttt atttttctct tttcgttgtt tgctgtccag
    82321 ataattgcaa ccgtcgtgcc agaaaattaa ttaagcagct taatttttta attcattgtt
    82381 tttaatagat taaatcttta cgtccgtaaa cggagatttc ccgcaaagcc aatttaccga
    82441 taatgaaata tcgtctttta taaggatatc taagatgcgt aaaacagtgg cttttggttt
    82501 tgtcggaacg gtgttggatt atgccgggcg cggcagtcag cgctggtcaa aatggcgtcc
    82561 gtcactctgt ctatgccagc aagaatcgtt agtcatcaat cgactggaat tgttgcacga
    82621 cgcccgctca cgctcgctgt ttgaaacgct taaacgcgat atcgccaatg tttcgccaga
    82681 aacaaaagtg gtgggcgttg agattgaact gcataaccca tgggatttcg aggaggttta
    82741 cgcctgcctg catgatttcg cccgtggtta cgcgttcgaa cctgataaag aagactattt
    82801 aattcacatc accaccggta cccacgtcgc gcagatttgc tggtttctgc tggcggaagc
    82861 acgttacctg cccgccaggt taatacaatc ttcgccaccg cgtaaaaaag aacagccgcg
    82921 cggtgcaggt gaagtgacga ttatcgatct cgatttaagc cgttataacg ccatcgccag
    82981 ccgctttgcc gaggaacgcc agcaaacgct cgattttctt aagtccggca ttgccacgcg
    83041 t
//
LOCUS       DAMZFT010000006        80519 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.006, whole genome shotgun sequence.
ACCESSION   DAMZFT010000006 DAMZFT010000000
VERSION     DAMZFT010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 80519)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 80519)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..80519
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.006"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..474
                     /locus_tag="PN977_000469"
     CDS             <1..474
                     /locus_tag="PN977_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001226973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl CoA:acetate/3-ketoacid CoA transferase"
                     /protein_id="HDI6040550.1"
                     /translation="YSGGGIDITFLGMGEMDQYGNVNVSHLNGNLIGPGGFLEIAQNA
                     RKVVFCGTFDAKGSKIDVTPDGLHIAQSGQIPKLVTQVEKITFSAAYAQQSGQEVLYI
                     TERAVFQLTAEGVELIEIAPGVEIERDILPYMAFRPIINQPRLMESSLFTPMEDA"
     gene            471..2552
                     /locus_tag="PN977_000470"
     CDS             471..2552
                     /locus_tag="PN977_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005766390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HDI6040551.1"
                     /translation="MSQLDDTLLDALTHVTFPKGFVSAEPASIVTVEDVDYPIWQVDA
                     LVVGSGAAGLRAAVELKRRQQNVLIATAGLYMGTSACSGSDKQTLFTAATAGNGDNFT
                     KLAEALASGGAMDHDTAYVEAVGSLHTLGGLQYLGLELPEDRYGAILRYQTDHDEAGR
                     ATSCGPRTSRLMVKVLLEEVQRLAIPMLTSATVIKLLHQRDENGEDRVAGAILATGHR
                     AHNPWGLAIVTAPNVVLATGGPGELYRDSVYPHKCFGSLGLALEEGLTLTNLTESQFG
                     IGTTRSTFPWNLSGTYVQVIPYIYSVDAGGNEYNFLADYYRTTQELASNIFRKGYQWP
                     FHATRVMDFGSSLLDMAVAQEQQSGRQVFMDFNRNPEPVPGDLPFSLDRLDDDVRAYL
                     ENNDALAPSPIERLQRMNPLSISLYKMHGYDLTTQPLQFAMNNQHMNGGIEVDIWGQT
                     SLPGCFAVGEVAGTHGVTRPGGAALNAGQVFAVRLARFIGCTQKRNIDGDIAQLAAPA
                     LASIREIITQAHDNGTGMPLSVVREKIQARMSDHAGFICHADKVRRATRDALLLNEFV
                     QRHGLAIKHVGEVAELFMWRHMTLTSAAVLTQLTHYIDAGGGSRGARIILDRDGNSIP
                     QTRNGFCDAWRFRSERTEDKKDKLLIHYCNSIFHVRETPVREFPTIRGIWFEKNWPGF
                     LNGTIYQPQDE"
     gene            2573..3049
                     /locus_tag="PN977_000471"
     CDS             2573..3049
                     /locus_tag="PN977_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012311688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HDI6040552.1"
                     /translation="MRNIIRSINALLATLNITILAIIVACVTWQVVARFIFKSPSIFT
                     DELSRLLFICLGLFGGAYTAGQNRHLAIDLLPMMLKGKARRHLFLCIQIIVIIFSTII
                     MVYGGGLLTLDTFDSGQTSPALGWQMGYIYMSIPISGVLIIIYTIDMVLTELKQPL"
     gene            3092..4393
                     /locus_tag="PN977_000472"
     CDS             3092..4393
                     /locus_tag="PN977_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000386845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HDI6040553.1"
                     /translation="MDYWLPIIVLFGAFFFMLALGVPIVYAIGLSTLASISTQLDFNS
                     ALSVVSQKLASGLDSFTLLAIPFFILSGNIMNHGGIARRLINFARILGGRLPGSLAHC
                     NILANMLFGAISGSAVASAAAMGGVMHPQQVKEGYDPAFSTAVNVASAPTGLLIPPSN
                     TLIVYSLVSGGTSIAALFLAGYVPGILLGLALMVIAGIIAVRRGYPKPERPTLRQAGV
                     AIWMAIPSIFLIILIMGGVLSGIFTPTEASAIAVIYTLFLALVLYREISVKDLPKIFL
                     ESVITTAIVLLLIGSSMGMSWAMSNADVPFLILDLLNTISDNPIIILLIINIILLIIG
                     TFMDMTPAVLIFTPIFLPVVTELGMDPIHFGIVMVLNMCIGICTPPVGSVLFVGCSVS
                     KLPINKIIKPMLPFYAVMVLVLAMVTYIPQISMALPRALGY"
     gene            4446..5417
                     /locus_tag="PN977_000473"
     CDS             4446..5417
                     /locus_tag="PN977_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001422748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HDI6040554.1"
                     /translation="MKIKVSAGIIGAVLMLSASQSWAVTLKLSHNQDKSHPVHKAMEF
                     FAKKSKEYSNGDITIRIYPNGTLGTQRETMELIRSGAIPLVKTNAAEMEAFENSYKLF
                     SLPYLFRDRDHYYQVMQGDIGRKILDSTKSKGYFGLTFYDGGARSFYGNKPVLKPDDL
                     KGMKVRVQPSPGAVEMIKVMGGNPTPLDYGELYTALQQGVVDMAENSVMALTTMRHGE
                     VAKSFSLDEHTMVPDVVLMSNAAFDKLSPENQAVILKAAKESMSYMKDLWSEEEKQEF
                     AKLDKMGVKVYQVDKAPFIEKVQPMYANFAKDNPALAPMLADIQAAK"
     gene            complement(5529..5978)
                     /gene="rplI"
                     /locus_tag="PN977_000474"
     CDS             complement(5529..5978)
                     /gene="rplI"
                     /locus_tag="PN977_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HDI6040555.1"
                     /translation="MQVILLDKVANLGSLGDQVNVKAGYARNFLVPQGKAVPATKKNI
                     EFFEARRAELEAKLAEVLAAANARAEKINALETVTIASKAGDEGKLFGSIGTRDIADA
                     VTAAGVEVAKSEVRLPNGVLRTTGEHEVSFQVHSEVFAKVIVNVVAE"
     gene            complement(6020..6247)
                     /gene="rpsR"
                     /locus_tag="PN977_000475"
     CDS             complement(6020..6247)
                     /gene="rpsR"
                     /locus_tag="PN977_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012986941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HDI6040556.1"
                     /translation="MARYFRRRKFCRFTAEGVQEIDYKDIATLKNYITESGKIVPSRI
                     TGTRAKYQRQLARAIKRARYLSLLPYTDRHQ"
     gene            complement(6252..6566)
                     /gene="priB"
                     /locus_tag="PN977_000476"
     CDS             complement(6252..6566)
                     /gene="priB"
                     /locus_tag="PN977_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N"
                     /protein_id="HDI6040557.1"
                     /translation="MTNRLVLSGTVCRTPLRKVSPSGIPHCQFVLEHRSVQEEAGFHR
                     QAWCQMPVIVSGHENQAITHSITVGSRITVQGFISCHKAKNGLSKMVLHAEQIELIDS
                     GD"
     gene            complement(6573..6968)
                     /gene="rpsF"
                     /locus_tag="PN977_000477"
     CDS             complement(6573..6968)
                     /gene="rpsF"
                     /locus_tag="PN977_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HDI6040558.1"
                     /translation="MRHYEIVFMVHPDQSEQVPGMIERYTAAITGAEGKIHRLEDWGR
                     RQLAYPINKLHKAHYVLMNVEAPQEVIDELETTFRFNDAVIRSMVMRTKHAVTEASPM
                     VKAKDERRERRDDFANETADDAEAGDSEE"
     gene            7296..7571
                     /locus_tag="PN977_000478"
     CDS             7296..7571
                     /locus_tag="PN977_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HDI6040559.1"
                     /translation="MFSRVLALLAVLLLSANTWAAIEINNHQARNMDDVQSLGVIYIN
                     HNFATESEARQALNEETDAQGATYYHVILMREPGSNGNMHASADIYR"
     gene            complement(7700..8386)
                     /gene="ulaF"
                     /locus_tag="PN977_000479"
     CDS             complement(7700..8386)
                     /gene="ulaF"
                     /locus_tag="PN977_000479"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710063.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase UlaF"
                     /protein_id="HDI6040560.1"
                     /translation="MQKLKQQVFEANMDLPRYGLVTFTWGNVSAIDRERGLVVIKPSG
                     VAYETMKAADMVVVDMSGKVVEGEYRPSSDTATHLELYRRYPSLGGIVHTHSTHATAW
                     AQAGLAIPALGTTHADYFFGDIPCTRGLSEEEVQGEYELNTGKVIIETLGDAEPLHTP
                     GIVVYQHGPFAWGKDAHDAVHNAVVMEEVAKMAWIARSINPQLNHIDSYLMNKHFMRK
                     HGPNAYYGQK"
     gene            complement(8386..9240)
                     /gene="ulaE"
                     /locus_tag="PN977_000480"
     CDS             complement(8386..9240)
                     /gene="ulaE"
                     /locus_tag="PN977_000480"
                     /EC_number="5.1.3.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase UlaE"
                     /protein_id="HDI6040561.1"
                     /translation="MLSKQIPLGIYEKALPAGECWLERLQLAKKLGFDFVEMSVDETD
                     DRLSRLDWSREQRLALVNAIVETGVRVPSMCLSAHRRFPLGSEDDAVRAQGLEIMRKA
                     IQFAQDVGIRVIQLAGYDVYYQEANNETRRRFRDGLKESVEMASRAQVTLAMEIMDYP
                     LMNSISKALGYAHYLNNPWFQLYPDIGNLSAWDNDVQMELQAGIGHIVAVHVKDTKPG
                     VFKNVPFGEGVVDFERCFETLKQSGYCGPYLIEMWSETAEDPAAEVAKARDWVKARMA
                     KAGMVEAA"
     gene            complement(9250..9900)
                     /gene="ulaD"
                     /locus_tag="PN977_000481"
     CDS             complement(9250..9900)
                     /gene="ulaD"
                     /locus_tag="PN977_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HDI6040562.1"
                     /translation="MSLPMLQVALDNQTMDSAYETTRLIAEEVDIIEVGTILCVGEGV
                     RAVRDLKALYPHKIVLADAKIADAGKILSRMCFEANADWVTVICCADINTAKGALDVA
                     KEFNGDVQIELTGYWTWEQAQQWRDAGIQQVVYHRSRDAQAAGVAWGEADITAIKRLS
                     DMGFKVTVTGGLALEDLPLFKGIPIHVFIAGRSIRDAASPVEAARQFKRSIAELWG"
     gene            complement(9914..10378)
                     /gene="ulaC"
                     /locus_tag="PN977_000482"
     CDS             complement(9914..10378)
                     /gene="ulaC"
                     /locus_tag="PN977_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIA"
                     /protein_id="HDI6040563.1"
                     /translation="MKLRDSLAENKSIRLQAEANTWQEAVKIGVDLLVAADVVEPRYY
                     QAILDGVEQFGPYFVIAPGLAMPHGRPEEGVKKTGFSLVTLKKPLEFNHEDNDPVDIL
                     ITMAAVDANTHQEVGIMQIVNLFEDEENFDRLRACRTEQEVLDLIDRTNAAA"
     gene            complement(10388..10693)
                     /gene="ulaB"
                     /locus_tag="PN977_000483"
     CDS             complement(10388..10693)
                     /gene="ulaB"
                     /locus_tag="PN977_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIB"
                     /protein_id="HDI6040564.1"
                     /translation="MTVRILAVCGNGQGSSMIMKMKVDQFLTQSNIDHTVNSCAVGEY
                     KSELSGADIIIASTHIAGEITVTGNKYVVGVRNMLSPADFGPKLLEVIKAHFPQDVK"
     gene            complement(10709..12106)
                     /gene="ulaA"
                     /locus_tag="PN977_000484"
     CDS             complement(10709..12106)
                     /gene="ulaA"
                     /locus_tag="PN977_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418614.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HDI6040565.1"
                     /translation="MEILYNIFTVFFNQVMTNAPLLLGIVTCLGYILLRKSVSVIIKG
                     TIKTIIGFMLLQAGSGILTSTFKPVVAKMSEVYGINGAISDTYASMMATIDRMGDAYS
                     WVGYAVLLALALNICYVLLRRITGIRTIMLTGHIMFQQAGLIAVTLFIFGYSMWTTII
                     CTAILVSLYWGITSNMMYKPTQEVTDGCGFSIGHQQQFASWIAYKVAPFLGKKEESVE
                     DLKLPGWLNIFHDNIVSTAIVMTIFFGAILLSFGIDTVQAMAGKVNWTVYILQTGFSF
                     AVAIFIITQGVRMFVAELSEAFNGISQRLIPGAVLAIDCAAIYSFAPNAVVWGFMWGT
                     IGQLIAVGILVACGSSILIIPGFIPMFFSNATIGVFANHFGGWRAALKICLVMGMIEI
                     FGCVWAVKLTGMSAWMGMADWSILAPPMMQGFFSIGIAFMAVIIVIALAYMFFAGRAL
                     RAEEDAEKQLAEQSA"
     gene            12461..13525
                     /gene="ulaG"
                     /locus_tag="PN977_000485"
     CDS             12461..13525
                     /gene="ulaG"
                     /locus_tag="PN977_000485"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003856017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HDI6040566.1"
                     /translation="MSKVKSITRESWILSTFPEWGSWLNEEIEQEQVAPGTFAMWWLG
                     CTGIWLKSEGGTNVCVDFWCGTGKQSHGNPLMKQGHQMQRMAGVKKLQPNLRTTPFVL
                     DPFAIRQIDAVLATHDHNDHIDVNVAAAVMQNCADDVPFIGPKTCVDLWIGWGVPKER
                     CIVVKPGDVVKVKDIEIHALDAFDRTALITLPADQKAAGVLPDGMDDRAVNYLFKTPG
                     GTLYHSGDSHYSNYYAKHGNEHQIDVALGSYGENPRGITDKMTSADILRMGEALNAKV
                     VIPFHHDIWSNFQADPQEIRVLWEMKKDRLKYGFKPFIWQVGGKFTWPLDKDNFEYHY
                     PRGFDDCFTIEPDLPFKSFL"
     gene            13633..14388
                     /gene="ulaR"
                     /locus_tag="PN977_000486"
     CDS             13633..14388
                     /gene="ulaR"
                     /locus_tag="PN977_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator UlaR"
                     /protein_id="HDI6040567.1"
                     /translation="MTEAQRHQILLEMLAQLGFVTVEKVVERLGISPATARRDINKLD
                     ESGKLKKVRNGAEAITQQRPRWTPMNLHQAQNHDEKVRIAKAASQLVNTGESVVINCG
                     STAFLLGREMCGKPVQIITNYLPLANYLIDQEHDSVIIMGGQYNKSQSITLSPQGSEN
                     SLYAGHWMFTSGKGLTAEGLYKTDMLTAMAEQKMLSVVGKLVVLVDSSKIGERAGMLF
                     SRADQIDMLITGKNANPEILQQLEAQGVSILRV"
     gene            complement(14385..15134)
                     /gene="yjfP"
                     /locus_tag="PN977_000487"
     CDS             complement(14385..15134)
                     /gene="yjfP"
                     /locus_tag="PN977_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HDI6040568.1"
                     /translation="MIEIESRELADIPVLHAYPVGQKDTPLPCAIFYHGFTSSSLVYS
                     YFAVALAQAGLRVIMPDAPDHGSRFSGDAARRLNQFWQILLQSMQEFTTLRAAIAEEN
                     WLLDDRLAVGGASMGAMTALGITARHPTVKCTASMMGSGYFTLLARSLFPPLIPETAA
                     QQNEFNNIVAPLAEWEATNHLEQLGDRPLLLWHGLDDDVVPADESLRLQQALSETGRD
                     KLLTCSWQPGVRHRITPEALDAAVTFFRQHL"
     gene            15316..15645
                     /gene="bsmA"
                     /locus_tag="PN977_000488"
     CDS             15316..15645
                     /gene="bsmA"
                     /locus_tag="PN977_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313192.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm peroxide resistance protein BsmA"
                     /protein_id="HDI6040569.1"
                     /translation="MVSRKRNSVIYRFASLLLVLMLSACSALQGTPQPAPPVTDHPQE
                     IRRDQTQGLQRIGSVSTMVRGSPDDALAEIRAKAVAAKADYYVVVMVDETIVTGQWYS
                     QAILYRK"
     gene            15794..16069
                     /gene="yjfN"
                     /locus_tag="PN977_000489"
     CDS             15794..16069
                     /gene="yjfN"
                     /locus_tag="PN977_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418609.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease activator YjfN"
                     /protein_id="HDI6040570.1"
                     /translation="MKQLLASPSLQLVTYPASATAQSAEFASADCVTGLNEIGQISVS
                     NISGDPQDVERIVALKADEQGASWYRIITMYEDQQPDNWRVQAILYA"
     gene            complement(16186..17811)
                     /locus_tag="PN977_000490"
     CDS             complement(16186..17811)
                     /locus_tag="PN977_000490"
                     /EC_number="1.3.8.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418608.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isovaleryl-CoA dehydrogenase"
                     /protein_id="HDI6040571.1"
                     /translation="MHWQTHTVFNQPIPLNNSNLYLSDGALCEAVTREGAGWDSDFLA
                     SIGQQLGTAESLELGRLANVNPPELLRYDAQGRRLDDVRFHPAWHLLMQALCTNRVHN
                     LAWEEDARSGAFVARAARFMLHAQVEAGSLCPITMTFAATPLLLQMLPAPFQDWTTPL
                     LSDRYDSHLLPGGQKRGLLIGMGMTEKQGGSDVMSNTTRAERLEDGSYRLVGHKWFFS
                     VPQSDAHLVLAQTTGGLSCFFVPRFLPDGQRNAIRLERLKDKLGNRSNASCEVEFQDA
                     IGWLLGQEGEGIRLILKMGGMTRFDCALGSHAMMRRAFSLAIYHAHQRHVFGNPLIQQ
                     PLMRHVLSRMALQLEGQTALLFRLARAWDRRADAKEALWARLFTPAAKFVICKHGIPF
                     VAEAMEVLGGIGYCEESELPRLYREMPVNSIWEGSGNIMCLDVLRVLNKQAGVYDLLS
                     EAFVEVKGQDRYFDRAVRRLQQQLRKPAEELGREITHQLFLLGCGAQMLKYASPPMAQ
                     AWCQVMLDTRGGVRLSEQIQNDLLLRATGGVCL"
     gene            complement(17895..19058)
                     /locus_tag="PN977_000491"
     CDS             complement(17895..19058)
                     /locus_tag="PN977_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HDI6040572.1"
                     /translation="MLRHNVPVRRDLDRIAADNGFDFHIIDNEIYWDESRAYRFTLRQ
                     IEEQIEKPTAELHQMCLEVVDRAVKDEEILTQLAIPPLYWDVIAESWHARDPSLYGRM
                     DFAWCGNAPVKLLEYNADTPTSLYESAYFQWLWLEDARRSGVIPRDADQYNAIQERLI
                     SRFSELYSREPFYFCCCQDTDEDRSTVLYLQDCAQQAGQESRFIYIEDLGLGVGGVLT
                     DLDDNVIQRAFKLYPLEWMMRDDNGPLLRKRREQWVEPLWKSILSNKGLMPLLWRFFP
                     GHPNLLASWFEGEKPQIAAGESYVRKPIYSREGGNVTIFDGQNNVVDHADGDYADEPM
                     IYQAFQPLPRFGDSYTLIGSWIVDDEACGMGIREDNTLITKDTSRFVPHYIAG"
     gene            complement(19061..19699)
                     /locus_tag="PN977_000492"
     CDS             complement(19061..19699)
                     /locus_tag="PN977_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 domain-containing protein"
                     /protein_id="HDI6040573.1"
                     /translation="MARKRKSRNNSKIGHGAISRIGRPNNPFEPRRNRYAQKYLTLAL
                     MGGAAFFVLKGCGDSGDVDNDGDGTFYSTVQDCIDDGNNSDICAHGWNNAKAAFYADV
                     PKNMTQQNCQSKYENCYYDNVEQSWIPVVSGFLLSRVIRKDRDEPFVYNSGGSSFASR
                     PVWRNTSGDYSWRSGSGKKESYSSGGFTTKKASTVSRGGYGRSSSARGHWGG"
     gene            complement(19709..20107)
                     /locus_tag="PN977_000493"
     CDS             complement(19709..20107)
                     /locus_tag="PN977_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF350 domain-containing protein"
                     /protein_id="HDI6040574.1"
                     /translation="MHILDSLLAFSAYFFIGVAMVIIFLFIYSKITPHNEWQLIKNNN
                     TAASLAFSGTLLGYVIPLSSAAINAVSIPDYFAWGGIALVIQLLVFAGVRLYMPALSE
                     KIINHNTAAGMFMGTAALAGGIFNAACMTW"
     gene            complement(20125..20784)
                     /locus_tag="PN977_000494"
     CDS             complement(20125..20784)
                     /locus_tag="PN977_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjfK family protein"
                     /protein_id="HDI6040575.1"
                     /translation="MSGFFQRLFGKDNKPAIARGPLGLHLNSGFTLDTLAFRLLEDEL
                     LIALPGEEFTVAAVSRIDLGGGSQIFRYYTSGDEFLQINTTGGENIDDIDDIKLFVYE
                     ESYGISKESHWREAINAKAMGAITLNWQEKRWQRFFNSEEPGNIEPVYMLEKVENQNH
                     AKWEVHNFTMGYERQVTEDTCEYLLLNGEESFNDLGEPEWLFSRALGVDIPLTSLHII
                     G"
     gene            complement(20834..21532)
                     /locus_tag="PN977_000495"
     CDS             complement(20834..21532)
                     /locus_tag="PN977_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspA/IM30 family protein"
                     /protein_id="HDI6040576.1"
                     /translation="MGILKSLFTLGKSFISQAEESIEETQGVRMLEQHIRDAKAELDK
                     AGKSRVDLLARVKLSHDKLKDLRERKASLEARALEALSKNVNPSLINEVAEEIARLEN
                     LITAEEQVLSNLEVSRDGVEKAVTATAQRIAQFEQQMEVVKATEAMQRAQQAVTTSTV
                     GASSSVSTAAESLKRLQTRQAERQARLDAAAQLEKVADGRDLDEKLAEAGIGGSNKSI
                     AQDVLARLQRQQGE"
     gene            complement(21551..21952)
                     /locus_tag="PN977_000496"
     CDS             complement(21551..21952)
                     /locus_tag="PN977_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2170 family protein"
                     /protein_id="HDI6040577.1"
                     /translation="MTWNPLALATALQTVPEQNIDVTNSESALIIKMNDYGDLQINIL
                     FTSRQMIIETFICPVSSISNPDEFNTFLLRNQKMMPLSSVGISSVQQEEYYIVFGALS
                     LKSSLEDILLEITSLVDNALDLAEITEEYSH"
     gene            complement(22079..22810)
                     /gene="rlmB"
                     /locus_tag="PN977_000497"
     CDS             complement(22079..22810)
                     /gene="rlmB"
                     /locus_tag="PN977_000497"
                     /EC_number="2.1.1.185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HDI6040578.1"
                     /translation="MSEMIYGIHAVQALLERAPERFQEVFILKGREDKRLLPLIHALE
                     SQGVVIQLANRQYLDEKSDGAVHQGIIARVKPGRQYQENDLPDLIASLDQPFLLILDG
                     VTDPHNLGACLRSADAAGVHAVIVPKDRSAQLNATAKKVACGAAESVPLIRVTNLART
                     MRMLQEENIWIVGTAGEADHTLYQSKMTGRLALVMGAEGEGMRRLTREHCDELISIPM
                     AGSVSSLNVSVATGICLFEAVRQRS"
     gene            complement(22991..25432)
                     /gene="rnr"
                     /locus_tag="PN977_000498"
     CDS             complement(22991..25432)
                     /gene="rnr"
                     /locus_tag="PN977_000498"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418600.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HDI6040579.1"
                     /translation="MSQDPFQEREAEKYANPIPSREFILEHLTKREKPASRDELAVEL
                     HIEGEEQLEGLRRRLRAMERDGQLVFTRRQCYALPERLDLVKGTVIGHRDGYGFLRVE
                     GRKDDLYLSSEQMKTCIHGDQVLAQPLGADRKGRREARIVRVLVPKTSQIVGRYFTEA
                     GVGFVVPDDSRLSFDILIPPDQIMGARMGFVVVVELTQRPTRRTKAVGKIVEVLGDNM
                     GTGMAVDIALRTHEIPYIWPQAVEQQVAGLKEEVPEEAKAGRVDLRDLPLVTIDGEDA
                     RDFDDAVYCEKKRGGGWRLWVAIADVSYYVRPPTPLDREARNRGTSVYFPSQVIPMLP
                     EVLSNGLCSLNPQVDRLCMVCEMTVSSKGRLTGYKFYEAVMSSHARLTYTKVWHILQG
                     DQDLREQYAPLVKHLEELHNLYKVLDKAREERGGISFESEEAKFIFNAERRIERIEQT
                     QRNDAHKLIEECMILANISAARFVEKAKEPALFRIHDKPSTEAITSFRSVLAELGLEL
                     PGGNKPEPRDYAELLESVADRPDAEMLQTMLLRSMKQAIYDPENRGHFGLALQSYAHF
                     TSPIRRYPDLTLHRAIKYLLAKEQGHQGNTTETGGYHYSMEEMLQLGQHCSMAERRAD
                     EATRDVADWLKCDFMLDQVGNVFKGVISSVTGFGFFVRLDDLFIDGLVHVSSLDNDYY
                     RFDQVGQRLMGESSGQTYRLGDRVEVRVEAVNMDERKIDFSLISSERAPRNVGKTARE
                     KAKKGDAGKKGGKRRQVGKKVNFEPDSAFRGEKKTKPKAAKKDARKAKKPSAKTQKIA
                     AATKAKRAAKKKVAE"
     gene            complement(25471..25896)
                     /gene="nsrR"
                     /locus_tag="PN977_000499"
     CDS             complement(25471..25896)
                     /gene="nsrR"
                     /locus_tag="PN977_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide-sensing transcriptional repressor
                     NsrR"
                     /protein_id="HDI6040580.1"
                     /translation="MQLTSFTDYGLRALIYMASLPEGRMTSISEVTDVYGVSRNHMVK
                     IINQLSRAGYVTAVRGKNGGIRLGKPASAIRIGDVVRELEPLSLVNCSSEFCHITPAC
                     RLKQALSKAVQSFLTELDNYTLADLVEENQPLYKLLLVE"
     gene            complement(26101..27399)
                     /gene="purA"
                     /locus_tag="PN977_000500"
     CDS             complement(26101..27399)
                     /gene="purA"
                     /locus_tag="PN977_000500"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HDI6040581.1"
                     /translation="MGNNVVVLGTQWGDEGKGKIVDLLTERAKYVVRYQGGHNAGHTL
                     VINGEKTVLHLIPSGILRENVTSIIGNGVVLSPAALMKEMKELEDRGIPVRERLLLSE
                     ACPLILDYHVALDNAREKARGAKAIGTTGRGIGPAYEDKVARRGLRVGDLFDKETFAE
                     KLKEVMEYHNFQLVNYYKAEAVDYQKVLDDTMAVADILTSMVVDVSDLLDQARQRGDF
                     VMFEGAQGTLLDIDHGTYPYVTSSNTTAGGVATGSGLGPRYVDYVLGILKAYSTRVGA
                     GPFPTELFDETGEFLCKQGNEFGATTGRRRRTGWLDTVAVRRAVQLNSLSGFCLTKLD
                     VLDGLKEVKLCVAYRMPDGREVTTTPLAADDWKGVEPIYETMPGWSESTFGVKDRSGL
                     PQAALNYIKRIEELTGVPIDIISTGPDRTETMILRDPFDA"
     gene            complement(27503..27700)
                     /locus_tag="PN977_000501"
     CDS             complement(27503..27700)
                     /locus_tag="PN977_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2065 domain-containing protein"
                     /protein_id="HDI6040582.1"
                     /translation="MNSTIWLALALVLVLEGLGPMLYPKAWKKMISAMTNLPDNILRR
                     FGGGLVVAGVVVYYMLRKTIG"
     gene            complement(27782..28786)
                     /gene="hflC"
                     /locus_tag="PN977_000502"
     CDS             complement(27782..28786)
                     /gene="hflC"
                     /locus_tag="PN977_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HDI6040583.1"
                     /translation="MRKSVIAIIIIVLVVLYMSVFVVKEGERGITLRFGKVLRDDDNK
                     PLVYEPGLHFKIPFIETVKMLDARIQTMDNQADRFVTKEKKDLIVDSYIKWRISDFSR
                     YYLATGGGDISQAEVLLKRKFSDRLRSEIGRLDVKDIVTDSRGRLTLEVRDALNSGSA
                     GTEDEVTTPAADNAIAEAAERVTAETKGKVPVINPNSMAALGIEVVDVRIKQINLPTE
                     VSEAIYNRMRAEREAVARRHRSQGQEEAEKLRATADYEVTRTLAEAERQGRIMRGEGD
                     AEAAKLFADAFSKDPDFYAFIRSLRAYENSFSGNQDVMVMSPDSDFFRYMKTPTSATR
                     "
     gene            complement(28789..30048)
                     /gene="hflK"
                     /locus_tag="PN977_000503"
     CDS             complement(28789..30048)
                     /gene="hflK"
                     /locus_tag="PN977_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HDI6040584.1"
                     /translation="MAWNQPGNNGQDRDPWGSSKPGGNSEGNGNKGGRDQGPPDLDDI
                     FRKLSKKLGGLGGGKGTGSGGGSSSQGPRPQLGGRVVTIAAAAIVIIWAASGFYTIKE
                     AERGVVTRFGKFSHLVEPGLNWKPTFIDEVKPVNVEAVRELAASGVMLTSDENVVRVE
                     MNVQYRVTDPEKYLYSVTSPDDNLRQATDSALRGVIGKYTMDRILTEGRTVIRSDTQR
                     ELEETIRPYDMGITLLDVNFQAARPPEEVKAAFDDAIAARENEQQYIREAEAYTNEVQ
                     PRANGQAQRILEEARAYKAQTILEAQGEVARFAKLLPEYKAAPEITRERLYIETMEKV
                     LGNTRKVLVNDKGGNLMVLPLDQMLKGGNAPAAKSDNGASNLLRLPPASSSTTSGASN
                     TSSTSQGDIMDQRRANAQRNDYQRQGE"
     gene            complement(30134..31414)
                     /gene="hflX"
                     /locus_tag="PN977_000504"
     CDS             complement(30134..31414)
                     /gene="hflX"
                     /locus_tag="PN977_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HDI6040585.1"
                     /translation="MFDRYDAGEQAVLVHIYFTQDKDMEDLQEFESLVSSAGVEALQV
                     ITGSRKAPHPKYFVGEGKAVEIAEAVKATGASVVLFDHALSPAQERNLERLCECRVID
                     RTGLILDIFAQRARTHEGKLQVELAQLRHLATRLVRGWTHLERQKGGIGLRGPGETQL
                     ETDRRLLRNRIVQIQSRLERVEKQREQGRQSRIKADVPTVSLVGYTNAGKSTLFNRIT
                     EARVYAADQLFATLDPTLRRIDVADVGETVLADTVGFIRHLPHDLVAAFKATLQETRQ
                     ATLLLHVIDAADVRVQENIEAVNTVLEEIDAHEIPTLLVMNKIDMLEDFEPRIDRDEE
                     NKPIRVWLSAQTGAGIPQLFQALTERLSGEVAQHTLRLPPQEGRLRSRFYQLQAIEKE
                     WMEEDGSVSLQVRMPIVDWRRLCKQEPALIDYLI"
     gene            complement(31490..31798)
                     /gene="hfq"
                     /locus_tag="PN977_000505"
     CDS             complement(31490..31798)
                     /gene="hfq"
                     /locus_tag="PN977_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HDI6040586.1"
                     /translation="MAKGQSLQDPFLNALRRERVPVSIYLVNGIKLQGQIESFDQFVI
                     LLKNTVSQMVYKHAISTVVPSRPVSHHSNNAGGGTSSNYHHGSSAQNTSAQQDSEETE
                     "
     gene            complement(31884..32834)
                     /gene="miaA"
                     /locus_tag="PN977_000506"
     CDS             complement(31884..32834)
                     /gene="miaA"
                     /locus_tag="PN977_000506"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HDI6040587.1"
                     /translation="MSDISKASLPKAIFLMGPTASGKTALAIELRKILPVELISVDSA
                     LIYKGMDIGTAKPNAEELLAAPHRLLDIRDPSQAYSAADFRRDALAEMADITAAGRIP
                     LLVGGTMLYFKALLEGLSPLPSADPEVRARIEQQAAEQGWESLHRQLQEIDPVAAARI
                     HPNDPQRLSRALEVFFISGKTLTELTQTSGDALPYQVHQFAIAPASRELLHQRIEQRF
                     HQMLASGFEAEVRALFARGDLHTDLPSIRCVGYRQMWSYLEGEISYDEMVYRGVCATR
                     QLAKRQITWLRGWEGVHWLDSEKPEQARDEVLQVVGAIAG"
     gene            complement(32827..34674)
                     /gene="mutL"
                     /locus_tag="PN977_000507"
     CDS             complement(32827..34674)
                     /gene="mutL"
                     /locus_tag="PN977_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HDI6040588.1"
                     /translation="MPIQVLPPQLANQIAAGEVVERPASVVKELVENSLDAGATRIDI
                     DIERGGAKLIRIRDNGCGIKKDELALALARHATSKIASLDDLEAIISLGFRGEALASI
                     SSVSRLTLTSRTAEQQEAWQAYAEGRDMDVTVKPAAHPVGTTLEVLDLFYNTPARRKF
                     LRTEKTEFNHIDEIIRRIALARFDVTINLSHNGKIVRQYRAVPEGGQKERRLGAICGT
                     AFLEQALAIEWQHGDLTLRGWVADPNHTTPALAEIQYCYVNGRMMRDRLINHAIRQAC
                     EDKLGADQQPAFVLYLEIDPHQVDVNVHPAKHEVRFHQSRLVHDFIYQGVLSVLQQQL
                     ETPLPLDDEPQPAPRAIPENRVAAGRNHFAEPAVREPVAPRYTPAPASGSRPAAPWPN
                     AQPGYQKQQGEVYRQLLQTPAPMQKPKAPEPQEPALAANSQSFGRVLTIVHSDCALLE
                     RDGNISLLALPVAERWLRQAQLTPGEAPVCAQPLLIPLRLKVSGEEKSALEKAQSALA
                     ELGIDFQSDAQHVTIRAVPLPLRQQNLQILIPELIGYLAKQSVFEPGNIAQWIARNLM
                     SEHAQWSMAQAITLLADVERLCPQLVKTPPGGLLQSVDLHPAIKALKDE"
     gene            complement(34684..36021)
                     /gene="amiB"
                     /locus_tag="PN977_000508"
     CDS             complement(34684..36021)
                     /gene="amiB"
                     /locus_tag="PN977_000508"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiB"
                     /protein_id="HDI6040589.1"
                     /translation="MMYRIRNWLVATLLLLCAQVGAATLSDIQVSNGNQQARITLSFI
                     GDPDYAFSHQSKRIVALDIKQTGVIQGLPLLFSGNNLVKAIRSGTPKDAQTLRLVVDL
                     TENGKTEAVKRQNGSNYTVVFTINADAPPPPPPPPVVAKRVETPAVGAPRVSEPARNP
                     FKTESNRTTGVISSNTVTRPAARATANTGDKIIIAIDAGHGGQDPGAIGPGGTREKNV
                     TIAIARKLRTLLNDDPMFKGVLTRDGDYFISVMGRSDVARKQNANFLVSIHADAAPNR
                     SATGASVWVLSNRRANSEMASWLEQHEKQSELLGGAGDVLANSQSDPYLSQAVLDLQF
                     GHSQRVGYDVATSMISQLQRIGEIHKRRPEHASLGVLRSPDIPSVLVETGFISNNSEE
                     RLLASDDYQQQLAEAIYKGLRNYFLAHPMQSAPQGATAQTASTVTTPDRTLPN"
     gene            complement(36040..36501)
                     /gene="tsaE"
                     /locus_tag="PN977_000509"
     CDS             complement(36040..36501)
                     /gene="tsaE"
                     /locus_tag="PN977_000509"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HDI6040590.1"
                     /translation="MMNRVIPLPDEQATLDLGERVAKACDGATVIYLYGDLGAGKTTF
                     SRGFLQALGHQGNVKSPTYTLVEPYTLDNLMVYHFDLYRLADPEELEFMGIRDYFAND
                     AICLVEWPQQGTGVLPDPDVEIHIDYQAQGREARVSAVSSAGELLLARLAG"
     gene            complement(36473..38020)
                     /gene="nnr"
                     /locus_tag="PN977_000510"
     CDS             complement(36473..38020)
                     /gene="nnr"
                     /locus_tag="PN977_000510"
                     /EC_number="4.2.1.136"
                     /EC_number="5.1.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ADP-dependent NAD(P)H-hydrate
                     dehydratase/NAD(P)H-hydrate epimerase"
                     /protein_id="HDI6040591.1"
                     /translation="MTDHTMKKNPVSIPHTVWHADDIRRGEREAVDALGLTLYELMLR
                     AGEAAFQVCRSAYPDARHWLVLCGHGNNGGDGYVVARLAKAVGIDVTLLAQESDKPLP
                     EEAALAREAWLNAGGEIHASNIVWPESVDLIVDALLGTGLQQAPRESISQLIDHANSH
                     PAPIVAVDIPSGLLAETGATPGAVINADHTITFIALKPGLLTGKARDVTGQLHFDSLG
                     LDSWLAGQETKIQRFSAEQLSHWLKPRRPTSHKGDHGRLVIIGGDHGTAGAIRMTGEA
                     ALRAGAGLVRVLTRSENIAPLLTARPELMVHELTMDSLTESLEWADVVVIGPGLGQQE
                     WGKKALQKVENFRKPMLWDADALNLLAINPDKRHNRVITPHPGEAARLLGCSVAEIES
                     DRLHCAQRLVQRYGGVAVLKGAGTVVAAHSDALGIIDVGNAGMASGGMGDVLSGIIGA
                     LLGQKMSPYDAACAGCVAHGAAADVLAARFGTRGMLATDLFSTLQRIVNPEVTDKNHD
                     ESSNSAP"
     gene            38019..39158
                     /gene="queG"
                     /locus_tag="PN977_000511"
     CDS             38019..39158
                     /gene="queG"
                     /locus_tag="PN977_000511"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HDI6040592.1"
                     /translation="MSEPLDLNQLAQKIKQWGLELGFQQVGITDTDLSASEPKLQAWL
                     DKQYHGEMDWMARHGMLRARPHELLPGTLRVISVRMNYLPANAAFASTLKNPKLGYVS
                     RYALGRDYHKLLRNRLKKLGEMIQQHCVSLNFRPFVDSAPILERPLAEKAGLGWTGKH
                     SLILNREAGSFFFLGELLVDIPLPVDQPVEEGCGKCVACMTICPTGAIVEPYTVDARR
                     CISYLTIELEGAIPVELRPLMGNRIYGCDDCQLICPWNRYSQLTTEDDFSPRKPLHAP
                     ELIELFAWSEEKFLKVTEGSAIRRIGHLRWLRNIAVALGNAPWDETILTALESRKGEH
                     PLLDEHIAWAIAQQIERRNACVVEVQLPKKQRLVRVIEKGLPRDA"
     gene            complement(39141..39194)
                     /locus_tag="PN977_000512"
     CDS             complement(39141..39194)
                     /locus_tag="PN977_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6040593.1"
                     /translation="MRFYFYSQAVDESGVTR"
     gene            complement(39428..39503)
                     /locus_tag="PN977_000513"
     tRNA            complement(39428..39503)
                     /locus_tag="PN977_000513"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(39468..39470),aa:Gly,seq:gcc)
     gene            complement(39539..39614)
                     /locus_tag="PN977_000514"
     tRNA            complement(39539..39614)
                     /locus_tag="PN977_000514"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(39579..39581),aa:Gly,seq:gcc)
     gene            complement(39772..39847)
                     /locus_tag="PN977_000515"
     tRNA            complement(39772..39847)
                     /locus_tag="PN977_000515"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(39812..39814),aa:Gly,seq:gcc)
     gene            complement(40058..40603)
                     /gene="orn"
                     /locus_tag="PN977_000516"
     CDS             complement(40058..40603)
                     /gene="orn"
                     /locus_tag="PN977_000516"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HDI6040594.1"
                     /translation="MSANENNLIWIDLEMTGLDPERDRIIEIATLVTDANLNILAEGP
                     TIAVHQSDEQLALMDDWNVRTHTASGLVERVKASTMGDREAELATLEFLKQWVPAGKS
                     PICGNSIGQDRRFLFKYMPELEAYFHYRYLDVSTLKELARRWKPEILDGFTKQGTHQA
                     MDDIRESVAELAYYREHFIKL"
     gene            40698..41750
                     /gene="rsgA"
                     /locus_tag="PN977_000517"
     CDS             40698..41750
                     /gene="rsgA"
                     /locus_tag="PN977_000517"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small ribosomal subunit biogenesis GTPase RsgA"
                     /protein_id="HDI6040595.1"
                     /translation="MSKNKLSKGQQRRVNANHQRRLKTSKEKPDYDDNLFGEPDEGIV
                     ISRFGMHADVESADGDVHRCNIRRTIRSLVTGDRVVWRPGKPAAEGVNVKGIVEAVHE
                     RTSVLTRPDFYDGVKPIAANIDQIVIVSAILPELSLNIIDRYLVACETLQIEPIIVLN
                     KIDLLDDEGMKFVNEQMDIYRNIGYRVLMVSSHTQDGLKPLEEALTGRISIFAGQSGV
                     GKSSLLNALLGLQKEILTNDVSDNSGLGQHTTTAARLYHFPHGGDVIDSPGVREFGLW
                     HLEPEQITQGFVEFHDYLGLCKYRDCKHDTDPGCAIREAVEEGKIAETRFENYHRILE
                     SMAQVKTRKNFSDTDD"
     gene            41846..42814
                     /gene="psd"
                     /locus_tag="PN977_000518"
     CDS             41846..42814
                     /gene="psd"
                     /locus_tag="PN977_000518"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313166.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HDI6040596.1"
                     /translation="MLNSFKLSLQYILPKLWLTRLAGWGASKRAGWLTKLVIDLFVKY
                     YKVDMKEAQKPDTASYRTFNEFFVRPLRDEVRPIDTDPNVLVMPADGVISQLGKIEED
                     KILQAKGHNYSLEALLAGNYLMADLFRNGTFVTTYLSPRDYHRVHMPCNGILREMIYV
                     PGDLFSVNHLTAQNVPNLFARNERVICLFDTEFGPMAQILVGATIVGSIETVWAGTIT
                     PPREGIIKRWTWPAGENDGSVALLKGQEMGRFKLGSTVINLFAPGKVNLVEQLESLSV
                     TKIGQPLAVSTETFVTPDAEPAPLPAEEIEAEHDASPLVDDKKDQV"
     gene            42836..46159
                     /gene="mscM"
                     /locus_tag="PN977_000519"
     CDS             42836..46159
                     /gene="mscM"
                     /locus_tag="PN977_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="miniconductance mechanosensitive channel MscM"
                     /protein_id="HDI6040597.1"
                     /translation="MRLIITFLMAWCLSWGAYAATAPDSKQISQELEQAKAAKPAQPE
                     VVEALQSALNALEERKGSLERIKQYQEVIDNYPKLSATLRAQLNNMRDEPRSVSPGMS
                     TDALNQEILQVSSQLLDKSRQAQQEQERAREIADSLNQLPQQQTDARRQLNEIERRLG
                     TLTGNSPLNQAQNFALQSDSARLKALVDELELAQLSANNRQELARLRSELAEKESQQL
                     DAYLQALRNQLNSQRQLEAERALESTELLAENSADLPKDIVAQFKINRELSAALNQQA
                     QRMDLVASQQRQAASQTLQVRQALNTLREQSQWLGSSNLLGEALRAQVARLPEMPKPQ
                     QLDTEMAQLRVQRLRYEDLLNKQPQLRQIHQADGQPLTAEQNRILEAQLRTQRELLNS
                     LLQGGDTLLLELTKLKVSNGQLEDALKEVNEATHRYLFWTSDVRPMTIAWPLEIAQDL
                     RRLISLDTFSQLGKASVMMLTSKETILPLFGALILVGCSIYSRRYFTRFLERSAAKVG
                     KVTQDHFWLTLRTLFWSILVASPLPVLWMTLGYGLREAWPYPLAVAIGDGVTATVPLL
                     WVVMICATFARPNGLFIAHFGWPRERVSRGMRYYLMSIGLIVPLIMALMMFDNLDDRE
                     FSGSLGRLCFILICGALAVVTLSLKKAGIPLYLNKEGSGDNITNHMLWNMMISAPLVA
                     ILASAVGYLATAQALLARLETSVAIWFLLLVVYHVIRRWMLIQRRRLAFDRAKHRRAE
                     MLAQRARGEEEAHHHSSPEGAIEVDESEVDLDAISAQSLRLVRSILMLIALLSVIVLW
                     SEIHSAFGFLENISLWDVTSTVQGVESLEPITLGAVLIAILVFIITTQLVRNLPALLE
                     LAILQHLDLTPGTGYAITTITKYLLMLIGGLVGFSMIGIEWSKLQWLVAALGVGLGFG
                     LQEIFANFISGLIILFEKPIRIGDTVTIRDLTGSVTKINTRATTISDWDRKEIIVPNK
                     AFITEQFINWSLSDSVTRVVLTIPAPADANSEEVTEILLTAARRCSLVIDNPAPEVFL
                     VDLQQGIQIFELRIYAAEMGHRMPLRHEIHQLILAGFHAHGIDMPFPPFQMRLESLNG
                     KQTGRTLTSAGKGRQAGSL"
     gene            complement(46190..46504)
                     /locus_tag="PN977_000520"
     CDS             complement(46190..46504)
                     /locus_tag="PN977_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjeO family protein"
                     /protein_id="HDI6040598.1"
                     /translation="MSARMFILCCIWFIVAFLWITITSALDKEWMIDGRGINNVCDVL
                     MYLEDDDTRDVGVIMTLPLFFPFLWFALWRKKRGWFMYATALAIFGYWLWQFFLRYQF
                     CL"
     gene            complement(46501..46815)
                     /locus_tag="PN977_000521"
     CDS             complement(46501..46815)
                     /locus_tag="PN977_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000340721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6040599.1"
                     /translation="MDDASRDPVITEDEIRELHFSAGDVAEIEQTVLSFVDTRHTRKV
                     AMVVGNTINTLKERDGPRWGNLPDIYCAYLIRCLVFRGELVGYGDLFRMRYSEIKRPI
                     IS"
     gene            complement(46878..48380)
                     /gene="yjeM"
                     /locus_tag="PN977_000522"
     CDS             complement(46878..48380)
                     /gene="yjeM"
                     /locus_tag="PN977_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418580.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/gamma-aminobutyrate family transporter
                     YjeM"
                     /protein_id="HDI6040600.1"
                     /translation="MPHTIKKMSLIGLILMIFTSVFGFANSPSAYYLMGYSAIPFYIF
                     SALLFFIPFALMMAEMGAAYRKEEGGIYSWMNNSVGPRFAFIGTFMWFSSYIIWMVST
                     SAKVWVPFSTFLYGSDMTQHWRIAGLEPTQVVGLLAVAWMILVTVVASKGINKIARIT
                     AVGGIAVMCLNLVLLLVSITILLLNGGHFAQDINFLASPNPGYQSGLAMLSFVVFAIF
                     AYGGIEAVGGLVDKTENPEKNFAKGIVFAAIVISIGYSLAIFLWGVSTNWQQVLSNGS
                     VNLGNITYVLMKSLGVTLGNALHFSPEASLSLGVWFARITGLSMFLAYTGAFFTLCYS
                     PLKAIIQGTPKALWPEPMTRLNTMGMPSIAMWMQCGLVTIFILLVSFGGGTASAFFNK
                     LTLMANVSMTLPYLFLALAFPFFKARQDLDRPFVIFKTHMSAMIATVVVVLVVTFANV
                     FTIIQPVVEAGDWDSTLWMIGGPVFFSLLAMAIYQNYCSRMANKPELALD"
     gene            complement(48599..49576)
                     /gene="epmA"
                     /locus_tag="PN977_000523"
     CDS             complement(48599..49576)
                     /gene="epmA"
                     /locus_tag="PN977_000523"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P--(R)-beta-lysine ligase"
                     /protein_id="HDI6040601.1"
                     /translation="MSETASWQPSASIPNLLKRAAIMAEIRRFFADRGVLEVETPCMS
                     QATVTDIHLVPFETRFVGPGHSQGMNLWLMTSPEYHMKRLLVAGCGPVFQLCRSFRNE
                     EMGRYHNPEFTMLEWYRPHYDMYRLMNEVDDLLQQVLDCPAAESLSYQQAFLRYLEID
                     PLSADKTQLREVAAKLDLSNVADTEEDRDTLLQLLFTFGVEPNIGKEKPTFVYHFPAS
                     QASLAQISTEDHRVAERFEVYYKGIELANGFHELTDAREQQQRFEQDNRKRAARGLPQ
                     HPIDQNLIEALKVGMPDCSGVALGVDRLVMLALGAETLAEVIAFSVDRA"
     gene            49901..51709
                     /gene="frdA"
                     /locus_tag="PN977_000524"
     CDS             49901..51709
                     /gene="frdA"
                     /locus_tag="PN977_000524"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase (quinol) flavoprotein
                     subunit"
                     /protein_id="HDI6040602.1"
                     /translation="MQTFQADLAIVGAGGAGLRAAIAAAQANPNAKIALISKVYPMRS
                     HTVAAEGGSAAVAQDHDSFEYHFHDTVAGGDWLCEQDVVDYFVHHCPTEMTQLELWGC
                     PWSRRPDGSVNVRRFGGMKIERTWFAADKTGFHMLHTLFQTSLQFPQIQRFDEHFVLD
                     ILVDDGHVRGLVAMNMMEGTLVQIRANAVVMATGGAGRVYRYNTNGGIVTGDGMGMAL
                     SHGVPLRDMEFVQYHPTGLPGSGILMTEGCRGEGGILVNKNGYRYLQDYGMGPETPLG
                     EPKNKYMELGPRDKVSQAFWHEWRKGNTISTPRGDVVYLDLRHLGEKKLHERLPFICE
                     LAKAYVGVDPVKEPIPVRPTAHYTMGGIETDQNCETRIKGLFAVGECSSVGLHGANRL
                     GSNSLAELVVFGRLAGEQAMERASTAGNGNDAAINAQVAGVEQRLKDLVNQDGGENWA
                     KIRDEMGMAMEEGCGIYRTPELMQKTIDKLAELQERFKRVRITDTSSVFNTDLLYTIE
                     LGHGLNVAECMAHSAMARKESRGAHQRLDEGCTERDDVNFLKHTLAFRDADGTTRLEY
                     SDVKITTLPPAKRVYGGEADAADKAEAANKKEKANG"
     gene            51702..52436
                     /gene="frdB"
                     /locus_tag="PN977_000525"
     CDS             51702..52436
                     /gene="frdB"
                     /locus_tag="PN977_000525"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase iron-sulfur protein"
                     /protein_id="HDI6040603.1"
                     /translation="MAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALGYI
                     KDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDGMKVEALANFPIERD
                     LVVDMTHFIESLEAIKPYIIGNSRTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACP
                     QFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKH
                     VDPAAAIQQGKVESSKDFLIATLKPR"
     gene            52447..52842
                     /gene="frdC"
                     /locus_tag="PN977_000526"
     CDS             52447..52842
                     /gene="frdC"
                     /locus_tag="PN977_000526"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HDI6040604.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FGLFALKNGPEAWAGFVDFLQNPVIVIINLITLAAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            52853..53212
                     /gene="frdD"
                     /locus_tag="PN977_000527"
     CDS             52853..53212
                     /gene="frdD"
                     /locus_tag="PN977_000527"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HDI6040605.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGVVTI"
     gene            53275..54408
                     /locus_tag="PN977_000528"
     CDS             53275..54408
                     /locus_tag="PN977_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HDI6040606.1"
                     /translation="MFKTTLCTLLITASCSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPTTKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMKTRVFQPLKLNHTWINVPSAEEKNYAWG
                     YREGKAVHVSPGALDAEAYGVKSTIEDMARWVQSNLKPFDINEKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDIIINGSDNKIALAARPVKPITPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVTAAWQILNTLQ"
     gene            54496..55029
                     /gene="blc"
                     /locus_tag="PN977_000529"
     CDS             54496..55029
                     /gene="blc"
                     /locus_tag="PN977_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HDI6040607.1"
                     /translation="MRLLPLVAAATAAFLVVACSSPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDRGMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(55026..55343)
                     /gene="sugE"
                     /locus_tag="PN977_000530"
     CDS             complement(55026..55343)
                     /gene="sugE"
                     /locus_tag="PN977_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HDI6040608.1"
                     /translation="MSWIILVIAGLLEVVWAVGLKYTHGFSRLTPSVITVMAMIVSMA
                     LLAWAMKSLPVGTAYAVWTGIGAVGAAITGIVLLGESANPMRLASLALIVLGIIGLKL
                     STH"
     gene            complement(55518..55664)
                     /gene="ecnB"
                     /locus_tag="PN977_000531"
     CDS             complement(55518..55664)
                     /gene="ecnB"
                     /locus_tag="PN977_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HDI6040609.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAAT
                     KAQQ"
     gene            complement(55775..55900)
                     /gene="ecnA"
                     /locus_tag="PN977_000532"
     CDS             complement(55775..55900)
                     /gene="ecnA"
                     /locus_tag="PN977_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein antitoxin entericidin A"
                     /protein_id="HDI6040610.1"
                     /translation="MMKRLIVLVLLASALLTGCNTARGFGEDIKHLGNSISRAAS"
     gene            complement(55952..56518)
                     /gene="efp"
                     /locus_tag="PN977_000533"
     CDS             complement(55952..56518)
                     /gene="efp"
                     /locus_tag="PN977_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HDI6040611.1"
                     /translation="MATYYSNDFRAGLKIMLDGEPYAVEASEFVKPGKGQAFARVKLR
                     RLLTGTRVEKTFKSTDSAEGADVVDMNLTYLYNDGEFWHFMNNETFEQLSADAKAIGD
                     NAKWLLDQAECIVTLWNGQPISVTPPNFVELEIVDTDPGLKGDTAGTGGKPATLSTGA
                     VVKVPLFVQIGEVIKVDTRSGEYVSRVK"
     gene            56560..57588
                     /gene="epmB"
                     /locus_tag="PN977_000534"
     CDS             56560..57588
                     /gene="epmB"
                     /locus_tag="PN977_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HDI6040612.1"
                     /translation="MAHIVTLNTPSREDWLTQLADVVTDPDELLRLLNIDADEKLLAG
                     RSAKKLFALRVPRSFIDRMEKGNPNDPLLRQVITSQDEFVVAPGFSTDPLEEQHSVVP
                     GLLHKYHNRALLLVKGGCAVNCRYCFRRHFPYAENQGNKRNWQIALEYVAAHPELDEM
                     IFSGGDPLMAKDHELDWLLTQLEAIPHIKRLRIHSRLPIVIPARITDALVERFSHSTL
                     QILLVNHINHANEIDETFRQAMAKLRRVGVTLLNQSVLLRDVNDNAQTLANLSNALFD
                     AGVMPYYLHVLDKVQGAAHFMVSDDEARQIMRELLTLVSGYLVPKLAREIGGEPSKTP
                     LDLQLRQQ"
     gene            57823..58692
                     /locus_tag="PN977_000535"
     CDS             57823..58692
                     /locus_tag="PN977_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjeJ family protein"
                     /protein_id="HDI6040613.1"
                     /translation="MAISIKGVNTGVIRKSNNFIALALKIKEPRNKESLFFLSVMELR
                     DLLIALESRLHQKHKLDAAARLQYEQARDKVIKKMAENIPEILVDELKNADINRRVNT
                     LELTDNQGENLTFVLTLHDGSTCELVINELQIEMLARAIIHAINNAEMRELALRITSL
                     LDFLPLYDVDCQDNGNLEYDTYSQPEWKHNLFNHYLAVLYRFKDKSGKEQFSGAVVKT
                     REATPGKEVEAITRRMLDFSPRLKKLAGVPCQVYVRTVAANNAQPLTQDQCLRALHHL
                     RVQSTSKTAPQAK"
     gene            complement(58742..59095)
                     /locus_tag="PN977_000536"
     CDS             complement(58742..59095)
                     /locus_tag="PN977_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4156 domain-containing protein"
                     /protein_id="HDI6040614.1"
                     /translation="MHVKYLAGIVGAALLMAGCGSSNELSAAGQSVRIVDEQPGAECQ
                     LIGTATGKQSNWLSGQHGEEGGSMRGAANDLRNQAAAMGGNVIYGISSPSQGMLSSFV
                     PTDSQIIGQVYKCPN"
     gene            complement(59230..60876)
                     /gene="groL"
                     /locus_tag="PN977_000537"
     CDS             complement(59230..60876)
                     /gene="groL"
                     /locus_tag="PN977_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120975.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HDI6040615.1"
                     /translation="MAAKDVKFGNDARVKMLRGVNVLADAVKVTLGPKGRNVVLDKSF
                     GAPTITKDGVSVAREIELEDKFENMGAQMVKEVASKANDAAGDGTTTATVLAQAIITE
                     GLKAVAAGMNPMDLKRGIDKAVTAAVEELKALSVPCSDSKAIAQVGTISANSDETVGK
                     LIAEAMDKVGKEGVITVEDGTGLQDELDVVEGMQFDRGYLSPYFINKPETGAVELESP
                     FILLADKKISNIREMLPVLEAVAKAGKPLLIIAEDVEGEALATLVVNTMRGIVKVAAV
                     KAPGFGDRRKAMLQDIATLTGGTVISEEIGMELEKATLEDLGQAKRVVINKDTTTIID
                     GVGEEAAIQGRVAQIRQQIEEATSDYDREKLQERVAKLAGGVAVIKVGAATEVEMKEK
                     KARVEDALHATRAAVEEGVVAGGGVALIRVASKLADLRGQNEDQNVGIKVALRAMEAP
                     LRQIVLNCGEEPSVVANTVKGGDGNYGYNAATEEYGNMIDMGILDPTKVTRSALQYAA
                     SVAGLMITTECMVTDLPKNDAADLGAAGGMGGMGGMGGMM"
     gene            complement(60920..61213)
                     /locus_tag="PN977_000538"
     CDS             complement(60920..61213)
                     /locus_tag="PN977_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000027827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HDI6040616.1"
                     /translation="MNIRPLHDRVIVKRKEVETKSAGGIVLTGSAAAKSTRGEVLAVG
                     NGRILENGEVKPLDVKVGDIVIFNDGYGVKSEKIDNEEVLIMSESDILAIVEA"
     gene            61489..62745
                     /gene="yjeH"
                     /locus_tag="PN977_000539"
     CDS             61489..62745
                     /gene="yjeH"
                     /locus_tag="PN977_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine/branched-chain amino acid
                     transporter"
                     /protein_id="HDI6040617.1"
                     /translation="MSGLKQELGLAQGIGLLSTSLLGTGVFAVPALAALVAGNNSLWA
                     WPVLIILVFPIAIVFAILGRHYPSAGGVAHFVGMAFGSRLERVTGWLFLSVIPVGLPA
                     ALQIAAGFGQAMFGWHSWQLLLAELGTLALVWYIGTRGASSSANLQTVIAGLIVALIV
                     AIWWAGDIKPANIPFPAPGNIELTGLFAALSVMFWCFVGLEAFAHLASEFKNPERDFP
                     RALMIGLLLAGLVYWGCTVVVLHFDAYGEQMAAAASLPKIVVQLFGVGALWIACVIGY
                     LACFASLNIYIQSFARLVWSQAQHNPDHYLARLSSRHIPNNALNAVLGCCVVSTLVIH
                     ALEINLDALIIYANGIFIMIYLLCMLAGCKLLQGRYRLLAVVGGLLCVLLLAMVGWKS
                     LYALIMLAGLWLFLPKRKTPENGITT"
     gene            complement(62761..63237)
                     /gene="fxsA"
                     /locus_tag="PN977_000540"
     CDS             complement(62761..63237)
                     /gene="fxsA"
                     /locus_tag="PN977_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein FxsA"
                     /protein_id="HDI6040618.1"
                     /translation="MRWLPFIAIFLYVYIEISIFIQVAHVLGVLLTLVLVIFTSVIGM
                     SLVRNQGFKNFVLMQQKMAAGENPAAEMIKSVSLIIAGLLLLLPGFFTDFLGLLLLLP
                     PVQKHLTVKLMPHLRFSRMPGGGFSAGTGGGNTFDGEYQRKDDDRDRLDHKDDRQD"
     gene            63574..65010
                     /gene="aspA"
                     /locus_tag="PN977_000541"
     CDS             63574..65010
                     /gene="aspA"
                     /locus_tag="PN977_000541"
                     /EC_number="4.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313147.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HDI6040619.1"
                     /translation="MSNNIRIEEDLLGTREVPADAYYGVHTLRAIENFYISNNKISDI
                     PEFVRGMVMVKKAAAMANKELQTIPKSVANAIIAACDEVLNNGKCMDQFPVDVYQGGA
                     GTSVNMNTNEVLANIGLELMGHQKGEYQYLNPNDHVNKCQSTNDAYPTGFRIAVYSSL
                     IKLVDAINQLREGFERKAVEFQDILKMGRTQLQDAVPMTLGQEFRAFSILLKEEVKNI
                     QRTAELLLEVNLGATAIGTGLNTPKEYSPLAVKKLAEVTGFPCVPAEDLIEATSDCGA
                     YVMVHGALKRLAVKMSKICNDLRLLSSGPRAGLNEINLPELQAGSSIMPAKVNPVVPE
                     VVNQVCFKVIGNDTTVTMAAEAGQLQLNVMEPVIGQAMFESVHILTNACYNLLEKCIN
                     GITANKEVCEGYVYNSIGIVTYLNPFIGHHNGDIVGKICAETGKSVREVVLERGLLTE
                     AELDDIFSVQNLMHPAYKAKRYTDESEQ"
     gene            65128..66429
                     /gene="dcuA"
                     /locus_tag="PN977_000542"
     CDS             65128..66429
                     /gene="dcuA"
                     /locus_tag="PN977_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuA"
                     /protein_id="HDI6040620.1"
                     /translation="MLVVELIIVLLAIFLGARLGGIGIGFAGGLGVLVLAAIGVKPGN
                     IPFDVISIIMAVIAAISAMQVAGGLDYLVHQTEKLLRRNPKYITILAPIVTYFLTIFA
                     GTGNISLATLPVIAEVAKEQGVKPCRPLSTAVVSAQIAITASPISAAVVYMSSVMEGH
                     GISYLHLLSVVIPSTLLAVLVMSFLVTMLFNSKLSDDPIYRKRLEEGLVELRGEKQIE
                     IKSGAKTSVWLFLLGVVGVVIYAIINSPSMGLVEKPLMNTTNAILIIMLSVATLTTVI
                     CKVDTDNILNSSTFKAGMSACICILGVAWLGDTFVSNNIDWIKDTAGEVIQGHPWLLA
                     VIFFFASALLYSQAATAKALMPMALALNVSPLTAVASFAAVSGLFILPTYPTLVAAVQ
                     MDDTGTTRIGKFVFNHPFFIPGTLGVALAVCFGFVLGSFML"
     gene            66545..66883
                     /gene="cutA"
                     /locus_tag="PN977_000543"
     CDS             66545..66883
                     /gene="cutA"
                     /locus_tag="PN977_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent cation tolerance protein CutA"
                     /protein_id="HDI6040621.1"
                     /translation="MLDEKSSNTASVVVLCTAPDEATAQDLAAKVLAEKLAACATLIP
                     GATSLYYWEGKLEQEYEVQMILKTTVSHQQALLECLKSHHPYQTPELLVLPVTHGDTD
                     YLSWLNASLR"
     gene            66859..68556
                     /gene="dsbD"
                     /locus_tag="PN977_000544"
     CDS             66859..68556
                     /gene="dsbD"
                     /locus_tag="PN977_000544"
                     /EC_number="1.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD"
                     /protein_id="HDI6040622.1"
                     /translation="MAQRIFTLILLLCSTSVFAGLFDAPGRSQFVPADQAFAFDFQQK
                     QHDLNLTWQIKDGYYLYRKQIRITPEHAKIADVQLPQGVWHEDEFYGKSEIYRDRLTL
                     PVTINQASAGATLTVTYQGCADAGFCYPPETKTVPLSEVVANNAASQPVSVPQQEQHT
                     AQLPFSALWALLIGIGIAFTPCVLPMYPLISGIVLGGKQRLSTARALLLTFIYVQGMA
                     LTYTALGLVVAAAGLQFQAALQHPYVLIGLAIVFTLLAMSMFGLFTLQLPSSLQTRLT
                     LMSNRQQGGSPGGVFVMGAIAGLICSPCTTAPLSAILLYIAQSGNMWLGGGTLYLYAL
                     GMGLPLMLITVFGNRLLPKSGPWMEQVKTAFGFVILALPVFLLERVIGDIWGLRLWSA
                     LGVAFFGWAFITSLQAKRGWMRVVQIILLAAALVSVRPLQDWAFGATHTVQTQTHLNF
                     TQIKTVDELNQALVEAKGKPVMLDLYADWCVACKEFEKYTFSDPQVQKALADTVLLQA
                     NVTANDAQDVALLKHLNVLGLPTILFFDGQGQEHPQERVTGFMDAETFSAHLRDRQP"
     gene            68593..69168
                     /locus_tag="PN977_000545"
     CDS             68593..69168
                     /locus_tag="PN977_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDI6040623.1"
                     /translation="MQREDVLGEALKLLELQGIANTTLEMVAERVDYPLDELRRFWPD
                     KEAILYDALRYLSQQIDAWRRQLMLDETQTAEQKLLARYQALSECVKNNRYPGCLFIA
                     ACTFYPDPGHPIHQLADQQKSAAYDFTHELLTTLEVDDPAMVAKQMELVLEGCLSRML
                     VNRSQADVDTAHRLAEDILRFARCRQGGALT"
     gene            69275..69350
                     /locus_tag="PN977_000546"
     tRNA            69275..69350
                     /locus_tag="PN977_000546"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:69308..69310,aa:Phe,seq:gaa)
     gene            69527..70717
                     /locus_tag="PN977_000547"
     CDS             69527..70717
                     /locus_tag="PN977_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001218738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDI6040624.1"
                     /translation="MALTARQVETARPKEKDYKLSDERGLYLLVKTTGARYWRLKYRI
                     AGKEKKLALGVYPDVSLAEARIKRDDARKIISEGGDPGEKKRKEKLTQKISATNTFHA
                     LATEWHQHKSLSWSESYARSVLEALDKDIFPYLGKRSVTDILPLEMLEILRRIEKRGS
                     LEKLRKVRQYCNQIFRYAIATGRATVNPASELTSTLAAPKAAHFPHLRADELPVFLRK
                     LAEYHGSPVTRMATNLLLLTGLRTIELRSAEWSEIDFDNALWTIPESRMKMRRKHVVP
                     LSRQATDILLQLKTFSGQYRLVFPGRCDINKPMSEASINMVLKRIGYDGRATGHGFRH
                     TMSTILHEQGFNSAWIEMQLAHVDKNAIRGTYNHAQYLDGRREMMQWYADYIDSLSRQ
                     ESQG"
     gene            71151..72194
                     /locus_tag="PN977_000548"
     CDS             71151..72194
                     /locus_tag="PN977_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HDI6040625.1"
                     /translation="MNDRLRFEVNDNQGRFVFPDTWFGPLLGEFEEVLDAYDTDEISE
                     TSYINKLRHLAQREPDFIDIHAHLAYAFLEQNAPRKALNAALKGLAAGNRLIPESFSG
                     EIIWMHPENRPYLRALYAAILANVHLQRHQDAVMLTDKILAYNPEDNQGARWLLGSEL
                     LRTGDHERAFSVLKKHADEFSPYWYELGLLHFLNGEQVKAATAFRRGFAANTYIAEML
                     CGNLHPFPLAVWHNYSAGPDTAEDYYATYSPLWGQYPEALLFVNWLYNHSSVLHERAE
                     IIKCAEMLMQEDDFEICESILRQQEKLRERIDETLSEKIVQKCRNMNGEYVWPWILPF
                     SAAGRKHSGIQYQ"
     gene            complement(72554..73738)
                     /locus_tag="PN977_000549"
                     /pseudo
     CDS             complement(72554..73738)
                     /locus_tag="PN977_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001608786.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
     gene            74325..74554
                     /locus_tag="PN977_000550"
                     /pseudo
     CDS             74325..74554
                     /locus_tag="PN977_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001546148.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            75585..76802
                     /locus_tag="PN977_000551"
     CDS             75585..76802
                     /locus_tag="PN977_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418699.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDI6040626.1"
                     /translation="MATAWYKQVNPPQRKALFSAWLGYVFDGFDFMMIFYILHIIKAD
                     LGITDIQATLIGTVAFIARPIGGGFFGAMADKYGRKPMMMWAIIIYSVGTGLSGIATN
                     LYMLAVCRFIVGLGMSGEYACASTYAVESWPKNLQSKASAFLVSGFSVGNIIAAQIIP
                     QFAEVYGWRNSFFIGLLPVLLVLWIRKSAPESQEWIEDKYKDKSTFLSVFRKPHLSIS
                     MIVFLVCFCLFGANWPINGLLPSYLADNGVNTVVISTLMTIAGLGTLTGTIFFGFVGD
                     KIGVKKAFVVGLITSFVFLCPLFFISVKNSSLIGLCLFGLMFTNLGIAGLVPKFIYDY
                     FPTKLRGLGTGLIYNLGATGGMAAPVLATYISGYYGLGVSLFIVTVAFSALLILLVGF
                     DIPGKIYKLSVAK"
     gene            76814..77932
                     /locus_tag="PN977_000552"
     CDS             76814..77932
                     /locus_tag="PN977_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HDI6040627.1"
                     /translation="MINYGVVGVGYFGAELARFMNMHDNAKITCVYDPENGENIAREL
                     QCINMSSLDALVSSKLVDCVIVATPNYLHKEPVIKAAKNKKHVFCEKPIALSYEDCVD
                     MVKACKEAGVTFMAGHIMNFFNGVQYARKLIKEGVIGEILSCHTKRNGWENKQERLSW
                     KKMKEKSGGHLYHHIHELDCVQHLLGEIPETVTMIGGNLAHSGPGFGNEDDMLFMTLE
                     FPSGKLATLEWGSAFNWPEHYVIINGTKGSIKIDMQETAGSLRIGGQTKHFLVHETQE
                     EDDDRRKGNMTSEMDGAIAYGHPGKKTPLWLASLIRKETLFLHNILCGAKPEEDYIDL
                     LNGEAAMSAIATADAATLSRSQDRKVKISEIIKHTSVM"
     gene            77975..78100
                     /locus_tag="PN977_000553"
     CDS             77975..78100
                     /locus_tag="PN977_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001491548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6040628.1"
                     /translation="MIKNFIFDNLIILAVPFMIKTSLKTNLIFFFLCVFVPRMAS"
     gene            78386..79598
                     /locus_tag="PN977_000554"
                     /pseudo
     CDS             78386..79598
                     /locus_tag="PN977_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558238.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     misc_feature    78667..78782
                     /locus_tag="PN977_000554"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01497"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="AL1L pseudoknot; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /pseudo
                     /db_xref="RFAM:RF01497"
     gene            79781..80404
                     /locus_tag="PN977_000555"
     CDS             79781..80404
                     /locus_tag="PN977_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HDI6040629.1"
                     /translation="MPAQIDYLADKYCFTELNESPRLCQQWQDVLEECRQTEAGPEER
                     LRIALLNVDYVTSFELPFRLLLTRTPQLIAALREEWDISQKNVVFNDKRFGCVYSLKA
                     SLSGVPDTFRYHLSHRIRRMVGNENTSSPYQQIAREVKVPRERLKYALEAGLLVTALD
                     GLFWSGSQRIAADVLRLRQNGMPVVTTNVEVHDNLTGTTRKVPAYHL"
BASE COUNT        19936 a        20878 c        19864 g        19841 t
ORIGIN      
        1 tacagtggcg gagggatcga tatcaccttc ctcggcatgg gagagatgga tcagtacggt
       61 aacgtcaacg tctcccatct caatggcaat ctgattggcc ccggcggatt tctcgaaatt
      121 gcgcaaaacg cccgtaaagt ggtgttctgc ggcacgttcg acgccaaagg tagcaagatt
      181 gatgtaacgc cagatggctt gcatatcgcc cagtcaggtc aaatccctaa actggttacc
      241 caggtggaaa aaatcacttt tagcgccgcc tacgcacagc aaagtggtca ggaagtgttg
      301 tatatcactg aacgtgcagt attccagtta acggcagaag gcgttgaatt aattgaaatc
      361 gcaccaggtg tggagattga gcgcgacatt ctgccgtata tggccttccg tccaattatc
      421 aatcagccac gcctgatgga aagtagcctg tttacgccga tggaggatgc atgagccagc
      481 ttgatgacac cctccttgat gcgctcacac acgtcacttt tccaaagggt tttgtgtcag
      541 cagagccagc gtcaattgtc accgttgagg atgttgatta cccgatctgg caagttgatg
      601 cgctggtcgt tggtagcggc gcagcaggat tacgtgcggc agttgaactt aaacgccgcc
      661 agcaaaatgt gctgatcgcc accgccgggt tatatatggg gacatcggca tgttctggct
      721 ctgataaaca aacactgttt accgccgcta ccgcgggcaa cggcgacaac ttcaccaaac
      781 tggcagaggc actggcgagc ggtggggcga tggatcacga caccgcttat gtcgaagcgg
      841 taggttctct gcacactcta ggcgggttgc aatatcttgg tctggaatta ccggaagatc
      901 gctatggcgc gattcttcgt tatcaaaccg accatgacga agccgggcgt gcaacctcgt
      961 gtgggccgcg tacctcaagg ttaatggtga aagtgctgct ggaagaagta cagcgtcttg
     1021 ctatcccaat gttgaccagt gcaacagtga ttaaactgct acatcagcgt gacgaaaacg
     1081 gcgaagaccg cgtggcgggg gcaatcctcg cgaccggtca tcgcgcccat aacccatggg
     1141 ggctggcaat tgtgactgcg cccaatgtgg tactggcaac aggagggcct ggcgagcttt
     1201 atcgcgacag tgtgtatcca cacaaatgtt tcggctcact ggggctggcg ctggaggaag
     1261 gtctgacgct caccaatctg accgaaagcc agtttggtat cggcacaacg cgcagcacct
     1321 ttccgtggaa tttgtccggg acttatgtac aggtgatccc atatatctat tccgtggatg
     1381 ccgggggtaa cgagtataac ttcctcgcgg attactatcg caccacccag gagctggctt
     1441 caaacatttt ccgtaaaggc tatcagtggc cgttccacgc cacgcgggta atggattttg
     1501 gctccagctt gttagatatg gcagtagcgc aagagcagca atcagggcgt caggtattta
     1561 tggatttcaa tcgtaatcct gaacctgtgc cgggtgacct gccgttctca ttagatcgac
     1621 tggacgacga cgttcgcgcg tatctggaaa ataacgacgc tctggcacca tcgcccatcg
     1681 aacgactgca acgaatgaat ccgctgtcta tctcgctgta taagatgcac ggttacgatc
     1741 tcaccacgca gccattgcag tttgccatga ataatcagca tatgaatggc ggcattgaag
     1801 tcgatatctg gggacaaaca tctctgcccg gttgttttgc agtgggggaa gttgctggca
     1861 cacacggcgt cacccgacct ggcggcgcgg cgttgaatgc cgggcaggtt tttgctgttc
     1921 gtctggcacg ttttattggt tgcacgcaaa agcggaatat tgatggagat atagcacagc
     1981 tggcagctcc ggcactggct tctataagag agatcattac tcaggcgcac gataacggga
     2041 ccgggatgcc gttgtcggtt gtgagagaaa aaattcaggc acgaatgtct gaccatgcgg
     2101 gatttatttg ccatgccgat aaagtccgac gtgccactcg tgatgccctg ctattgaacg
     2161 aatttgtgca acgacatgga ttggcaatca aacatgtggg tgaagttgcc gagctgttta
     2221 tgtggcggca tatgacgctg acttctgccg ccgtcttaac tcaactgaca cattatattg
     2281 atgcaggtgg tggtagccgg ggagcacgta ttattctcga cagggatgga aattctattc
     2341 cgcaaacacg aaatggtttt tgtgatgcct ggcgctttcg ttcagagcgt actgaggata
     2401 aaaaagataa actgcttatt cattattgca acagtatctt tcacgtcaga gaaacacctg
     2461 tccgtgagtt tcccactatc cgcggcattt ggtttgaaaa aaactggcct ggctttctta
     2521 atgggacaat ttaccagcca caggatgaat gatattgcaa aggttaatca ctatgcgaaa
     2581 tataattaga tctattaatg ctctgcttgc aacattgaat attaccattt tggcgatcat
     2641 tgtggcatgt gtgacatggc aagtggtagc acgatttata tttaaaagcc ccagtatatt
     2701 tacggatgaa ctcagtcgtt tgctttttat ttgccttggc ctttttggtg gtgcctatac
     2761 cgcggggcag aaccggcatc tggcgattga tctgcttcct atgatgctga aaggaaaagc
     2821 ccggcgtcat ttatttttgt gtattcagat tatcgttatt atcttctcta ccatcattat
     2881 ggtatatggt ggtgggctat taacgctgga cacattcgat tcaggtcaaa cctcacccgc
     2941 tttaggctgg cagatgggat atatctatat gagtatcccg attagtggtg ttcttattat
     3001 tatatacacc atagatatgg ttttaacaga acttaaacaa ccactctaaa gataataaac
     3061 aacaaccaac catagtagga atggagaaat tatggattac tggttaccga ttattgtgtt
     3121 atttggtgcc ttctttttta tgttggcatt aggtgtacct attgtttatg ccattggttt
     3181 atccacactg gcatcaattt ccacacaact cgatttcaac tcagcattga gtgtggtttc
     3241 ccaaaagtta gcctccgggt tagatagctt tacattattg gcaattccat tttttatttt
     3301 gtctggcaac attatgaatc atggaggaat agcgcgacga ttaatcaact ttgccagaat
     3361 tcttggcggt cgactgcctg gctcacttgc tcactgtaat attcttgcca atatgctgtt
     3421 cggggcaatt tctggttcag ctgttgcttc ggcggctgcg atgggcggcg tcatgcatcc
     3481 gcaacaggtt aaagagggct acgacccggc attcagtact gcggttaacg ttgcctctgc
     3541 cccgacgggt ttgcttattc caccaagtaa tacgttaatc gtctattcac tggtcagtgg
     3601 tggtacttct attgccgcac tgttcctggc gggttatgtt ccgggaattt tgctgggtct
     3661 ggcactgatg gtaatagccg gtattatcgc ggtacgtcgc ggttatccaa agccagaacg
     3721 cccgacttta cgccaggctg gcgttgcgat atggatggct atccccagta ttttcctgat
     3781 tattttaatt atggggggtg tattaagtgg tatttttacc ccgacagagg cctccgcaat
     3841 tgcagtaatt tatacccttt tccttgcact ggttctttat cgtgaaatct cagttaaaga
     3901 tttacctaaa atatttctgg aatccgttat taccactgca attgtcttgt tattaattgg
     3961 ttcttcgatg ggaatgtcat gggcgatgtc aaacgctgac gttcccttcc tgattctcga
     4021 tttgcttaac actatttctg acaacccaat aataatcctg cttattatca atattatcct
     4081 gttgattatc ggcactttta tggatatgac accggcggta ctgattttta cgccgatatt
     4141 tttaccggtt gtcaccgaac tgggcatgga cccgattcac tttggtattg tcatggtact
     4201 gaacatgtgt atcggtattt gtactccgcc tgtaggcagt gtgttatttg tcggttgtag
     4261 tgtatctaaa ttaccgatta ataaaattat caaacctatg ctgccatttt atgccgtgat
     4321 ggtattggtg ctggcaatgg tgacctatat tccgcaaatc agtatggcat tgccaagagc
     4381 actgggttac tgatgcgcat tcactggcgg cacctgccgc catatttatt agaaaaggaa
     4441 catctatgaa aataaaagta tccgctggca ttatcggtgc tgttcttatg ttatccgcaa
     4501 gccagtcctg ggcagtgaca ttaaaactga gtcataatca ggataagtct catcctgttc
     4561 ataaagcgat ggagttcttt gcgaaaaaga gcaaagagta ctctaacggt gatattacta
     4621 ttcgtattta tccaaatgga acattgggta ctcaacgaga aacaatggag ctgattcgtt
     4681 ctggcgctat tccactggta aaaaccaacg cggcagaaat ggaagcattt gaaaattcct
     4741 ataaattatt tagcctgcct tatttgttcc gcgatcgtga tcattattat caggtcatgc
     4801 agggcgatat cgggagaaaa atcctcgact caacgaaaag caaaggttat ttcgggctga
     4861 ctttttatga tggaggcgcc cgcagtttct acggcaataa accagtactg aaaccagacg
     4921 atctcaaagg catgaaagtc cgtgtccagc caagtcctgg cgcagttgaa atgatcaaag
     4981 tcatgggcgg taacccgacg ccactggatt acggcgagtt gtatacagcc ttacagcagg
     5041 gtgtggtcga tatggcagaa aacagcgtga tggcgctgac caccatgcgt cacggtgaag
     5101 tggcaaaatc cttcagcctt gacgaacaca ctatggttcc cgatgtggtt ctgatgagca
     5161 atgctgcgtt tgataaactt agcccggaaa atcaggcagt tatattaaaa gcagctaaag
     5221 aatcaatgag ctacatgaaa gacttgtgga gcgaggaaga gaaacaagaa tttgcaaaac
     5281 tggataaaat gggcgtgaaa gtctaccagg tagataaagc tccgtttatc gagaaagtac
     5341 agccaatgta cgcaaacttc gctaaggaca acccagccct tgccccaatg ctggctgata
     5401 ttcaggcagc taagtaatta tcttcgtagg gctgcttatt gtggccctta tcgatatctg
     5461 caagtgctcg ctacaaaagc aaaacgccga ccaatggtcg gcgtttttac gtctcgttga
     5521 ataacgaatt attcagctac tacgtttacg atcactttcg cgaatacttc gctgtgaacc
     5581 tggaagctca cttcgtgttc gccagtggta cgcagaacgc cgttcggcag acgaacttcg
     5641 ctcttagcca cttcaacgcc agctgcagtt acagcgtcag cgatgtcgcg agtaccgatg
     5701 gaaccgaaca gtttaccttc gtcgccagct ttagacgcga tggtaacagt ttccagtgca
     5761 ttgattttct cagcgcgagc attagctgct gccagaactt cagccagttt agcttccagt
     5821 tcagcgcgac gtgcttcgaa gaattcaatg tttttcttgg tagctggaac agctttaccc
     5881 tgcggtacca ggaagttacg agcatagccc gctttaacgt ttacctgatc acccaggcta
     5941 cccaggtttg ctactttatc aagcagaata acttgcatta ccttatcctc tcaaagtcgt
     6001 attaatggac cgtgaccgat tactgatggc gatcagtgta cggcagcagg gacaggtagc
     6061 gagcgcgttt gatagcgcga gccagctgac gctggtattt tgcacgggta ccggtgatac
     6121 ggcttgggac aatcttaccg ctttcggtga tgtagttttt cagcgtagcg atatctttat
     6181 agtcgatctc ttgaacgcct tccgcggtga aacggcagaa cttgcgacga cggaaataac
     6241 gtgccatatg gctagtctcc agaatctatc aattcaatct gctcggcatg caaaaccatt
     6301 ttgctcagtc cgttctttgc cttgtggcat gaaatgaacc cctgaacggt tatgcgactg
     6361 ccgaccgtta tactgtgagt aatggcctgg ttttcgtgtc cgctaacaat aacgggcatt
     6421 tggcaccacg cctgccggtg aaaaccggct tcctcctgca cagaacgatg ctcaagcacg
     6481 aactggcagt gaggaattcc tgatggactg acctttcgaa ggggagtcct gcacacggtg
     6541 ccggacaaca ccagacggtt ggtcatcaga aattactctt cagaatcccc agcttcagca
     6601 tcatcagcgg tttcgtttgc gaaatcatcg cgacgctcac ggcgctcgtc tttcgcttta
     6661 accatcggag atgcttcggt aacagcgtgc ttggtacgca taaccatgct gcggataacg
     6721 gcatcgttga agcggaaggt agtttccagc tcatcgatca cttcctgcgg agcttcaacg
     6781 ttcatcagaa cgtagtgtgc tttgtgcagt ttgttgatcg ggtaagccag ctgacggcgg
     6841 ccccagtctt ccagacggtg gatcttgcct tctgcaccag tgatggcagc agtgtagcgc
     6901 tcgatcatgc ccggaacctg ttcgctctgg tcaggatgga ccataaaaac gatttcgtaa
     6961 tgacgcatcg aattgctcct tacggattat tcagcctcct gtctgggtca gccgaatccc
     7021 ggggaggcaa ggaacgtgtt aaaggtcggc tgaaaaatga cgcgttatag tactgacata
     7081 ccccgctaaa ctcaaggcca gccgacaaat aaatctcatc tttcaccaaa tcgtactaac
     7141 acgctgattt gaagttcatc acacttcatc atggtgtttt tttgaacaac tgcatcagaa
     7201 atggtcacca tccacttctt tatcattgat taaaattaaa tcagaagatc accacaagag
     7261 gagagcacac aaagtcagcc gttgaggagg tatctatgtt cagtcgtgtt ttagcccttc
     7321 tggctgtgct tttgctaagt gcaaatacat gggcagccat tgaaattaat aaccaccagg
     7381 ccagaaatat ggacgatgtg caaagcttag gcgtgattta tatcaaccat aacttcgcca
     7441 ctgaaagtga agcacgtcag gcattaaatg aagagacaga tgcgcagggc gcaacgtact
     7501 accacgtgat tctgatgcgg gaaccgggga gtaacggcaa catgcacgcc agcgcggata
     7561 tttatcgcta gcaccaggta taaccaacga aacattgcca tagtttgctt tgcccccttc
     7621 gcagggggct ttttttgaaa aacggctgcg gaatcagacc agttatctcc cgaagaagga
     7681 aatttccgca gcgcgtgttt tacttctgcc cgtaataagc gttgggacca tgcttacgca
     7741 tgaagtgttt attcataaga tagctgtcga tgtgattcag ttgcgggttt atactgcgcg
     7801 caatccacgc cattttcgcc acttcttcca tcaccaccgc gttatgcacc gcatcgtggg
     7861 catctttccc ccaggcaaac ggcccgtgct gatacaccac aattcccggc gtatgcaacg
     7921 gctcggcgtc acccagcgtt tcgataatca ctttgccggt gttcagttcg tactcaccct
     7981 gcacctcttc ttcgcttaat ccgcgcgtac acgggatatc accaaagaag tagtcggcgt
     8041 gcgtagtgcc taacgccggg atcgccagcc ccgcctgcgc ccatgcggta gcatgagtgg
     8101 agtgggtatg gacaatgccg ccaagcgacg gataacgacg gtagagttcg agatgcgtcg
     8161 cggtgtcgga agacgggcga tattcccctt ccaccacctt gccgctcata tcaaccacca
     8221 ccatatcggc cgctttcatg gtttcgtagg caacgccgct gggcttgatc accaccagcc
     8281 cgcgttcgcg gtcaatggcg ctgacgttgc cccaggtaaa ggtcaccagc ccatagcgcg
     8341 gcagatccat gttggcttca aatacctgct gttttagctt ttgcattatg ccgcctccac
     8401 catgcccgct ttcgccatgc gcgctttcac ccaatcacgc gctttcgcca cttctgctgc
     8461 cgggtcttcc gccgtttcgc tccacatctc aatcaggtac ggcccgcaat agccactctg
     8521 tttgagcgtt tcgaagcaac gttcgaaatc cactacgcct tcgccaaacg gcacattttt
     8581 gaagacgcct ggtttggtgt ctttcacatg caccgcgacg atatgcccaa ttccggcctg
     8641 caattccatc tgcacgtcgt tgtcccacgc cgacaggttg ccgatatccg ggtagagctg
     8701 gaaccacggg ttgttgagat agtgcgcata gcccagcgcc ttgctgatgg agttcatcaa
     8761 tggataatcc atgatctcca ttgccagcgt cacctgcgcg cggcttgcca tctcaacgct
     8821 ctctttcagg ccgtcacgga aacgacgacg cgtttcgtta ttggcttcct gatagtaaac
     8881 gtcatagccc gccagttgga ttacgcgaat gccaacgtcc tgggcgaact ggatagcttt
     8941 acgcataatc tccagcccct gcgcccgtac cgcgtcatct tcgctgccca gcgggaaacg
     9001 acgatgagca gaaaggcaca tggacggcac gcgcacgccg gtttcaacaa tcgcattcac
     9061 cagcgccaga cgctgctcgc ggctccagtc gaggcgcgac aggcgatcgt cagtttcatc
     9121 taccgacatc tcgacaaaat cgaagcctaa tttttttgcc agttgcagcc gttccagcca
     9181 gcactccccg gcggggagcg ctttttcata gatgccaagc gggatttgtt tggacaacat
     9241 atccgctcct taaccccaca gttcggcgat ggaacgtttg aactgacgtg cggcttccac
     9301 cggagaagcg gcatcacgga tactacggcc cgcgataaag acgtgaatcg gaatgccttt
     9361 gaacagcggc agatcttcca gcgccaggcc accagtgacg gtcactttga agcccatatc
     9421 ggaaagacgt ttgatcgcgg taatgtccgc ttcgccccac gctacgcctg cggcctgtgc
     9481 gtcacggctg cggtgataaa ccacctgctg aatgcctgcg tcgcgccact gctgcgcctg
     9541 ttcccaggtc cagtaaccgg tcagttcgat ctgcacgtcg ccgttaaact cttttgccac
     9601 gtccagcgca cctttggcgg tgttgatatc cgcacagcaa atcacggtca cccagtcagc
     9661 gttggcttcg aagcacatgc gagaaaggat tttgcctgca tcggcaattt tggcgtcagc
     9721 cagtacgatt ttgtgcgggt acagcgcttt caggtcacga accgcacgca cgccttcgcc
     9781 tacgcaaaga atggtgccca cttcgataat gtcgacttct tcggcaatca ggcgagtggt
     9841 ttcgtaggcg ctatccatag tctggttgtc cagcgcgact tgcaacatcg gtaatgacat
     9901 tttcaattcc ttcttaagct gccgcgttgg tgcggtcaat gagatccagt acttcctgct
     9961 cggtacggca ggcacgtaaa cggtcgaaat tctcttcatc ttcaaacagg ttaacgatct
    10021 gcatgatgcc cacttcctgg tgagtgttgg catcgaccgc cgccatggtg atgaggatat
    10081 ccaccggatc gttatcttcg tggttgaact ccagcggctt tttcagcgtt accagagaga
    10141 aaccggtttt cttaacgccc tcttccggac gcccgtgcgg cattgccagt cccggtgcga
    10201 taacgaaata aggaccaaac tgttcaacgc catccagaat cgcctggtag taacgcggct
    10261 ctaccacatc tgccgctacc agcaggtcaa caccgatttt caccgcttcc tgccaggtat
    10321 ttgcttcggc ctgcaggcgg atagatttat tttcagccag cgaatcacgt aatttcatgg
    10381 cgcgtcctta cttcacatcc tgcgggaaat gcgctttgat cacttccagc agtttcgggc
    10441 caaagtcagc aggagagagc atgttgcgca cgccaaccac atatttgttg ccggtcacgg
    10501 tgatttcgcc cgcaatgtgc gtagaagcga tgatgatatc cgcgccgctc aactcgcttt
    10561 tgtattcgcc aaccgcgcag ctgtttaccg tgtggtcaat gtttgattgg gttaaaaact
    10621 ggtccacttt catcttcatg atcatggaac tgccttgtcc gttgccacac acagccagaa
    10681 tacgtacggt cataatcaaa actccttatt aagcagactg ttctgccagt tgtttttctg
    10741 catcttcttc tgcgcgcagt gcgcggccag cgaagaacat ataagccagt gcaattacaa
    10801 tgatgacagc cataaaggcg ataccgatgg agaagaagcc ctgcatcatc ggcggtgcca
    10861 gaatcgacca gtccgccata cccatccagg cactcatacc ggtaagtttc accgcccaga
    10921 cgcaaccaaa gatttcgatc atccccatca ccagacaaat cttcagcgcc gcacgccagc
    10981 cgccgaagtg gttagcgaac acgccgatgg tggcgttgga gaagaacatc gggataaagc
    11041 cggggataat caggatcgag gaaccgcagg cgaccaggat gccaaccgca atcagctgac
    11101 cgatggtgcc ccacataaag ccccagacca cggcgttcgg cgcgaagcta taaatagccg
    11161 cacagtcaat cgccagaacc gcgccaggga tcaggcgctg agagataccg ttaaacgctt
    11221 cagagagttc cgctacaaac atgcgcacac cctgagtgat gatgaagatc gccaccgcga
    11281 aggagaaacc ggtttgcagg atatacaccg tccagttcac tttgcctgcc atcgcctgca
    11341 cggtgtcgat accgaaggag agcagaatgg caccaaagaa gatggtcatg acaatcgccg
    11401 tggagacgat gttgtcgtgg aaaatattca gccagcctgg cagtttgagg tcttcaacgc
    11461 tttcctcttt tttgccaagg aacggcgcaa ccttataggc aatccatgat gcaaactgtt
    11521 gctggtgacc gatggagaaa ccgcagccgt ccgtcacttc ctgagtcggc ttgtacatca
    11581 tgttggaggt tatgccccag tagagcgaaa ccagaatcgc cgtgcagata atggtcgtcc
    11641 acatggaata gccgaaaata aacaacgtaa cggcaatcag tcccgcctgc tggaacatga
    11701 tgtgtccagt cagcatgatg gtacgaatac cggtaatgcg acgcagcagc acgtaacaga
    11761 tgttcagcgc cagcgctaac aacaccgcgt aacctaccca gctataggca tcgcccatgc
    11821 ggtcgatggt tgccatcatc gacgcgtaag tatcggaaat tgcgccgtta atgccgtaga
    11881 cttcggacat tttcgccacc accggtttga aggtgctggt gaggatgccg gaccctgcct
    11941 gcaacaacat gaaaccaatt atggttttaa tcgtgccttt aataataacg ctgacacttt
    12001 tgcgcagtag gatgtagccc agacaggtca caatacccag caacaacggg gcattggtca
    12061 tgacctggtt aaaaaacacg gtaaagatgt tgtagaggat ctccataacg atctcctgaa
    12121 gaagaggtaa ccgggtattc cacacacccg gaatgttgtg cattcactct aattttcaaa
    12181 agtaatcaca acaagattat ttatgattaa atgtgacgcg acccgcaaat aattccacat
    12241 ggatagagtg ggattcacac aacgaaataa ttattcattt aaattcattt agttaattga
    12301 attacacatt ccattaaatc tttccacact caaaatcaac aacaacttcc ctggcaaaaa
    12361 gtcaaaaaat ggtcattgcc ttcctgaaaa aatagtgtca ggattaatca aaaccaatca
    12421 catgttgatt cgaattgaac tatcatgaag gtaaatggcg atgagtaaag tgaaaagtat
    12481 cacccgagaa tcctggatcc tgagcacttt cccggagtgg ggtagctggc tgaatgaaga
    12541 aattgaacaa gagcaggtcg cccctggcac atttgcgatg tggtggctag gctgcaccgg
    12601 gatctggctg aaatcagaag gtggcaccaa cgtttgcgtt gatttctggt gcggcactgg
    12661 caaacaaagt cacggtaacc cgttaatgaa acagggtcac cagatgcagc gcatggctgg
    12721 cgtgaaaaaa ctgcagccaa acctgcgcac caccccgttt gttcttgatc cgtttgcgat
    12781 tcgccagatc gacgcggtac tggcgactca cgatcacaac gatcatatcg acgttaacgt
    12841 cgctgctgcc gtgatgcaga actgtgctga tgacgtaccg ttcattgggc cgaaaacctg
    12901 cgttgatttg tggattggct ggggcgtacc gaaagagcgt tgcatcgtcg ttaaaccggg
    12961 cgacgtagtg aaggtaaaag acattgaaat tcatgcactt gatgctttcg accgcacggc
    13021 actgatcacc ctgcctgccg atcaaaaagc agctggcgta ctgccggacg gcatggacga
    13081 tcgcgcggta aactacctgt tcaaaacccc aggcggtacc ctgtatcaca gcggcgattc
    13141 ccactactct aactactatg caaaacatgg taatgagcat cagatcgacg tggctttagg
    13201 ttcatacggc gaaaacccac gcggtattac cgacaaaatg accagtgctg atatcttgcg
    13261 tatgggcgaa gcgctgaatg cgaaagtggt tatcccgttc caccacgaca tctggtcaaa
    13321 cttccaggcc gatccgcaag aaatccgcgt gctgtgggag atgaaaaaag atcgtctgaa
    13381 gtatggcttc aagccgttta tctggcaggt gggcggcaaa tttacctggc cgctggataa
    13441 agacaacttc gagtaccact atccgcgtgg tttcgatgat tgcttcacta ttgaaccgga
    13501 tctgccgttc aagtcgttcc tgtaatctgt cgtttatacc ggatagcgtg gagtagttcg
    13561 cgctatccgg cgtagtattt cctggctatt tcaaatatca tctaaaaaaa tcaaatttta
    13621 tcggaatagc tcatgactga agcacaaaga catcaaatcc tcctggaaat gctcgcacaa
    13681 ttgggctttg tgaccgttga gaaagtcgtt gagcgtctgg gaatttcgcc tgccactgca
    13741 cgacgcgata tcaataaact tgacgaaagc ggcaaactga aaaaagtgcg caatggcgca
    13801 gaagctatta cccagcagcg cccacgctgg acgccgatga atctgcatca ggcgcagaat
    13861 cacgatgaaa aagtacgtat tgctaaagcg gcctcgcagc tggttaatac gggcgaaagc
    13921 gtagtcatca actgcggctc caccgcgttt ctgcttgggc gggaaatgtg tggcaagcca
    13981 gtgcaaatca tcactaatta tctaccgctg gcaaattacc tgatcgatca ggagcatgac
    14041 agcgtgatca ttatgggcgg acagtacaac aaaagtcagt ccatcacttt aagcccgcag
    14101 ggcagcgaaa acagtctcta tgccgggcac tggatgttta ccagcggcaa agggctgaca
    14161 gcagaagggt tgtataaaac cgatatgttg acggcaatgg cagagcagaa gatgctgagc
    14221 gtggtaggga aactggtggt actggttgat agcagtaaga ttggcgaacg cgcgggaatg
    14281 ctttttagcc gtgccgatca aatcgatatg ctgatcaccg gcaaaaatgc taacccggaa
    14341 atcctgcaac aactggaagc gcaaggggtc agcattctgc gtgtttaaag atgctggcgg
    14401 aaaaatgtca cggcagcatc taacgcctca ggcgtaatgc ggtgacgcac gccaggctgc
    14461 catgaacagg ttagcagttt atcccgtccc gtctcgctta aggcctgctg caaacgtagt
    14521 gattcgtcgg caggcacgac gtcatcgtca aggccatgcc acagcagtag aggtctgtca
    14581 ccaagttgtt ccaggtggtt tgtcgcttcc cactctgcca gcggcgcgac aatgttattg
    14641 aattcattct gctgtgctgc cgtttcaggt atcagcggtg gaaacagtga acgggcgagc
    14701 aatgtaaaat agcccgatcc catcatgctg gcggtacatt tcaccgtggg gtggcgagcg
    14761 gtaattccca gtgccgtcat cgcgcccatc gacgcgccac cgactgccag acggtcatca
    14821 agcagccagt tttcttcggc tattgccgca cgtaaagtag tgaattcctg catactttgt
    14881 agcaagattt gccagaattg atttaaccgc cgcgctgcgt cgccactaaa acgactaccg
    14941 tgatcgggcg catccggcat gatcacccgc aaaccagcct gcgccagcgc aacggcaaaa
    15001 tagctataca ccagactgga cgaagtaaag ccgtgataaa aaatcgcgca cggtaacggg
    15061 gtatcttttt gcccgacagg ataagcatga agaacgggaa tatctgccag ctcgcgtgat
    15121 tctatttcaa tcatctgtct ctcctttttg tctggtatga catgaagaaa tccttagcac
    15181 aagtttctgc tggcggtatt agttcataat gttgagatgt gggttacgct ttcatcacgt
    15241 tttcattcga aaatcgcgta ccaggtaaca attcgggaac attcccccaa agttaaatta
    15301 ataaggcact acactatggt tagcaggaaa cgtaatagcg ttatttaccg gtttgccagt
    15361 ttattattgg tgttgatgtt aagtgcctgt agcgcactgc aaggtacgcc acagccagca
    15421 ccgccagtaa ccgaccatcc gcaagagatt cgccgcgacc agacgcaagg gttacagcga
    15481 ataggtagcg taagcacgat ggttcggggt tctccggatg acgcattagc agaaatcaga
    15541 gcgaaagctg tcgctgcaaa agctgattat tacgttgtcg tcatggtgga cgaaaccatc
    15601 gtgacaggac agtggtattc acaagccatt ttgtaccgta aataaatcag acaacttaca
    15661 tagtcagctt tacattgctt tgcgtcccta tgcgtttacc tgtgcacaat aaattaccgg
    15721 ctggaagcct gggcgtttat taccgcgatg gaatgccctg caacatgtgg ggaaacgaaa
    15781 aatggagctg acgatgaaac aattacttgc ctcaccctcg ctgcaattag tgacttatcc
    15841 tgcgagcgcc acggcacagt ctgcagaatt cgccagtgct gactgcgtga cgggcctgaa
    15901 tgaaataggt cagatctcag ttagcaatat ttcaggggat ccgcaggacg tggaacgtat
    15961 cgtggcgtta aaggccgatg aacaaggtgc gtcatggtac cgcattatta ccatgtacga
    16021 agaccagcag cctgataact ggcgcgtaca ggcgatcctg tatgcgtgag tatcatctac
    16081 agcaaatgaa caatgccgga tgcggcgcga gcgccttgtc cggcctacaa ttttaaaata
    16141 tttcatttag ttaatcgaaa ccagcgtcgc atccgtcgta tacgcttaca aacacactcc
    16201 tcccgtcgcc cgcagcaata aatcattctg gatctgctct gacaaccgta cgccgccgcg
    16261 cgtatctaac atcacctgac accacgcctg cgccattggc ggagaggcat atttcaacat
    16321 ttgcgcaccg cagcccagca ggaatagctg atgagtaatc tctcgcccca attcttcagc
    16381 tggcttacgc agctgctgct gtaaacgacg aaccgcgcga tcaaaatagc gatcctgccc
    16441 tttcacttcc acaaatgctt ccgacaataa gtcgtatacg cccgcttgct tattgagaac
    16501 gcgcagcaca tccaggcaca taatattgcc ggaaccttcc caaatactgt ttaccggcat
    16561 ctcccggtaa agccgcggca gctcgctctc ctcgcaataa ccaatgccgc ccagcacctc
    16621 catcgcttcg gccacaaacg gaataccatg tttgcagatc acaaatttcg ccgcaggcgt
    16681 aaataaacgc gcccacagag cttctttggc atcggcacgc cggtcccacg ctcgcgcaag
    16741 acgaaacagc aacgccgttt gcccttcaag ctgaagcgcc atgcgactta agacatgacg
    16801 cataaggggc tgttggatca atggattacc aaaaacatgg cgttgatgtg catgataaat
    16861 cgccagcgaa aatgcacggc gcatcatggc atggctaccc agggcgcaat caaaacgcgt
    16921 catcccaccc attttcagga tcagacgaat tccttcccct tcctgcccca acaaccagcc
    16981 aatggcatcc tgaaactcca cttcgcagct ggcgttagag cgattaccca gcttatcttt
    17041 cagccgctcg aggcgaatcg cgttgcgttg cccgtcaggc aaaaagcgcg gcacaaaaaa
    17101 gcaggacaaa ccgcccgtag tctgcgccag caccagatgc gcatcgcttt gcggcaccga
    17161 gaaaaaccat ttatgcccca ccagccgata agagccatct tccaggcgct ctgcgcgggt
    17221 ggtgttgctc atgacatcgg aaccgccctg cttttccgtc attcccatgc caatcaacaa
    17281 accgcgtttt tgcccacctg gcaataagtg agaatcgtag cgatcgctca acagcggcgt
    17341 ggtccagtcc tgaaacggcg cgggtaacat ctgtaacagc aatggcgtgg cggcaaaggt
    17401 catcgttatc ggacataacg acccagcctc aacctgcgca tgtaacataa aacgcgccgc
    17461 gcgcgccaca aatgcgccgg agcgagcgtc ttcttcccag gcaagattgt gcacccgatt
    17521 ggtacatagc gcctgcatca gcaggtgcca ggcggggtga aaacgcacat cgtccagacg
    17581 gcgtccttgc gcatcgtagc gcaataattc aggcggattc acattcgcca gccgccccag
    17641 ttcaagggat tcagccgtcc ctaactgctg accaatactg gctagaaaat cgctatccca
    17701 gccagcacct tcacgcgtta ccgcttcgca gagcgcgcca tcagacaggt ataaattgct
    17761 gttatttaat ggtataggtt gattaaaaac ggtgtgagtt tgccagtgca ctgtgtctcc
    17821 ctccatcaat ggcagtcacc attagtatgg tcactgccat ggattcatga cagaaagaga
    17881 ttgctaaaac attcttatcc agcaatgtaa tgcggaacga aacgtgaggt gtctttggtg
    17941 atcaacgtgt tatcttcacg gatccccatt ccgcacgctt catcatcgac aatccagcta
    18001 ccgatgagtg tgtagctatc gccaaatcgc ggcagaggtt gaaacgcctg gtagatcatc
    18061 ggttcatcgg cgtaatcacc atcagcgtgg tcaacgacgt tattctgacc gtcgaaaatg
    18121 gtgacgtttc cgccttcgcg cgagtaaatc ggtttacgca catagctttc gccagcggca
    18181 atctgcggtt tttcaccctc aaaccaggat gcaagaaggt taggatgacc agggaagaag
    18241 cgccaaagca gcggcattag ccctttatta ctcaagatac ttttccataa cggctccacc
    18301 cattgctcac gacgcttgcg cagcagcgga ccgttatcgt cacgcatcat ccactccagc
    18361 ggatacagct taaatgcacg ctggatgaca ttatcatcaa gatcggtcag tactccgccg
    18421 acgcccaaac cgagatcttc aatgtagata aaccgcgact cctgccctgc ctgctgggcg
    18481 cagtcctgca agtacagcac ggtactcctg tcttcatcgg tgtcctgaca gcagcaaaaa
    18541 taaaacggtt cccgactgta aagctcacta aagcgcgaaa tcaggcgttc ctgaatggca
    18601 ttgtactgat cggcatcacg cggaataacg ccgctgcgcc gggcatcttc cagccacaac
    18661 cactggaaat aagccgactc gtacaatgaa gttggcgtat cggcgttgta ctccagcaac
    18721 ttaaccggtg cattaccaca ccaggcaaaa tccatacggc catacaacga aggatcgcgt
    18781 gcgtgccagc tttcggcgat cacatcccag tacaacggcg ggatcgccag ttgcgtcagg
    18841 atctcttcat ctttaaccgc acgatccacc acctccaggc acatctgatg cagttccgca
    18901 gtcggttttt cgatctgctc ttcaatctgg cgcaaagtaa agcgataagc ccgactctca
    18961 tcccaataga tttcattgtc gatgatatga aagtcaaatc cgttatcggc ggcaatccgg
    19021 tccagatccc gccgcacagg aacgttgtgt ctcagcatga ttagcctccc caatgcccac
    19081 gggcgctgga agaacgacca tagccgccgc gagaaacggt tgacgctttt ttggtggtaa
    19141 agccgcccga agagtaagac tcttttttgc cagaaccgga gcgccaggag taatcaccgg
    19201 aagtgttgcg ccagacgggg cgggaagcaa atgaggatcc gccgctgtta tagacgaacg
    19261 gctcatcgcg atctttacga ataacccggc ttaacaaaaa tccagaaacg accgggatcc
    19321 aactctgttc aacattgtcg tagtaacaat tttcatactt agactgacaa ttctgctgag
    19381 tcatattctt cggaacatcg gcataaaacg ccgctttggc gttgttccag ccatgagcac
    19441 agatatctga attattaccg tcatcaatac aatcctgcac agtcgagtaa aacgttccgt
    19501 cgccgtcgtt atcgacatcg ccactatcgc cacaaccttt caatacgaaa aaagcggcac
    19561 cgcccattag cgccaacgtt aagtattttt gtgcgtagcg attgcgacgc ggttcaaaag
    19621 gattattcgg tctgccaatg cgactgatcg ctccgtgacc aattttactg ttatttcttg
    19681 atttgcgttt tctggccatg atcgtccatt accatgtcat acaagctgcg ttaaaaatac
    19741 caccagccag tgcggcggtt cccataaaca ttcctgctgc ggtattgtga ttaataattt
    19801 tttcgcttaa tgcgggcata taaagtctga cgccagcaaa aacgagtaac tgaatcacca
    19861 gtgcaatccc gccccaggcg aaataatccg gaatactcac cgcattgatt gccgcactgg
    19921 ataaggggat cacgtaaccc aacaatgtac cgctgaatgc cagtgacgcc gcggtattat
    19981 tgtttttgat taattgccat tcgttgtgcg gtgtaatttt ggagtagata aacagaaaaa
    20041 taatcaccat agccacgcca ataaaaaaat aggcgctaaa ggcaagaagt gaatccagta
    20101 tatgcatcgt aaacgtcctt gtaattaacc aataatatga agtgatgtca gtgggatatc
    20161 aacacccagc gcgcgcgaaa ataaccactc cggctcgccg agatcgttaa aagattcctc
    20221 accatttaac agtaaatatt cacaggtatc ttcggtcacc tgccgttcat agcccatggt
    20281 aaaattatgc acttcccatt tggcgtggtt ttgattttca actttttcca gcatatagac
    20341 gggttcgata ttccccggtt cttcgctgtt aaaaaatcgc tgccagcgtt tttcctgcca
    20401 gtttaaggtt atagccccca tcgctttggc gttgatcgcc tcgcgccagt gactctcttt
    20461 actgataccg tagctctctt catagacaaa gagcttgata tcatcaatgt catcaatatt
    20521 ttcgccgccg gtggtattga tttgcagaaa ctcatcgcct gaagtgtagt agcggaaaat
    20581 ctggctaccg ccgcccagat cgatgcggct gacggcggct accgtaaatt cctcacccgg
    20641 cagcgcaatc agcaattcat cttccagcaa acgaaacgct aacgtgtcga gcgtaaagcc
    20701 actattgaga tgaagtccca gcggaccacg agcgatagca ggcttattat ccttgccaaa
    20761 cagacgctgg aaaaaaccag acatatttta tccttataat agttgccagc cttgcagtga
    20821 ggctggcaaa aaattactcg ccctgttggc gttgcagtct tgctaataca tcctgggcga
    20881 tacttttatt gctaccgcca attccggctt ccgccagctt ttcatcgaga tcgcgaccgt
    20941 ctgcgacttt ttccaactgt gcggcagcat ccaggcgagc ctgacgttcg gcctgacgcg
    21001 tttgcaggcg ttttaaggat tctgccgctg tcgaaacgct ggaagatgcg ccaacggtag
    21061 aggtcgttac agcctgttgt gcacgctgca tggcttcagt ggctttaacg acttccattt
    21121 gctgctcaaa ctgagcgata cgctgcgctg tcgctgtaac tgctttttcc acgccatcac
    21181 gagagacttc cagattcgac aacacttgct cttcagcggt aatgagattc tcaaggcgcg
    21241 cgatttcttc agcaacttcg ttaatcaacg acggattcac gttcttgctt aacgcttcca
    21301 gcgcacgagc ttccagactg gctttgcgct cacgtaaatc tttcagctta tcgtgactta
    21361 atttcactcg cgccagcaga tcaacgcgag attttccggc tttatcgagt tcagctttag
    21421 cgtcacgaat atgctgttcc agcatgcgca cgccctgggt ttcttcaatg gactcttccg
    21481 cctgggagat aaacgatttc cccagcgtaa ataaactttt taaaatcccc ataaataccc
    21541 gtccttgtaa ttagtgtgaa tattcttctg taatttcagc cagatccaat gcgttatcta
    21601 ccagcgaggt tatctcaagc agaatatctt caagagaaga tttaagcgat aacgcaccga
    21661 aaacaatgta atactcttcc tgttgcacgc tggagatccc taccgatgac aacggcatca
    21721 tcttctgatt tcttaataag aaggtattaa attcatctgg attgctgata ctacttaccg
    21781 gacaaataaa ggtttcgatg atcatttggc gggaagtaaa aagaatattg atttgcaaat
    21841 cgccataatc attcatttta ataattaatg cgctttcgct atttgttaca tcaatatttt
    21901 gttcaggtac agtttgcagc gccgtcgcca gcgccaacgg attccatgtc atatattatt
    21961 ccatatagat taagtttaaa tattaataaa atgaatattt acagtacgta attatcttac
    22021 cagctataga caaaaaaaaa ccatccaaat ctggatggct tttcataatt cagaggaact
    22081 agctgcgctg acgaaccgct tcaaataagc aaattccggt tgcaaccgaa acgttcaggg
    22141 aagaaacact tcctgccatt gggatgctga tcaactcatc gcaatgttca cgggtcaggc
    22201 gacgcatacc ttcaccttcc gcgcccatca ccagcgccag gcgtccggtc attttgctct
    22261 gatagagtgt atgatccgcc tcgcctgccg taccgacgat ccagatattc tcttcctgca
    22321 acatacgcat ggtgcgcgcc agattagtca cccgaatcag cggaacgctt tctgccgcac
    22381 cacaggccac ttttttcgcc gtggcgttga gctgtgcgga gcgatctttc ggcacaatca
    22441 ccgcatgaac accagcggcg tccgcgctac gcaggcacgc gccgaggttg tgcggatcgg
    22501 ttacgccgtc gaggatcagc aggaacggtt gatcgagcga agcgatcaga tccggcagat
    22561 cgttttcctg atactgccgt cctggcttca cacgagcgat aatgccctga tgcacggcac
    22621 cgtcgctttt ctcgtcgaga tattggcggt ttgccagctg gataaccacg ccctgggact
    22681 caagggcgtg aatcagcggc aacagacgtt tatcttcacg gccttttaaa ataaagactt
    22741 cctgaaaacg ttcaggggcg cgctccagca gggcctgcac tgcgtggatg ccgtaaatca
    22801 tttcgctcat taatgtactc gttgtttacg ttttgcctga tgcgctacgc ttatcaggcc
    22861 tacatgatcc ctgcaatgta ttgaatttgc gtgattatgt aggccggata aggcgttcac
    22921 gccgcatccg gcatgaacaa agcgcatttt gtcagcaatc taaccctctt ctgtttgaag
    22981 agggtaatga tcactctgcc acttttttct tcgccgcacg cttcgctttg gtcgctgcgg
    23041 ctattttctg cgttttcgcc gatggctttt tcgcttttct cgcgtctttc ttcgccgctt
    23101 tcggcttcgt ttttttctcg ccgcggaaag cgctgtctgg ctcaaagttt acctttttac
    23161 cgacctgacg acgcttgccg ccttttttac ctgcatcgcc ttttttcgct ttctcgcgcg
    23221 ccgttttacc gacgttgcgc ggtgcgcgtt cgctggagat caggctaaag tcgattttac
    23281 gctcgtccat attaaccgct tcgacgcgaa cttccacgcg atcgcccagg cgataagtct
    23341 ggccgctgga ttcacccatc aggcgttgcc ctacctggtc aaagcgatag tagtcattgt
    23401 ccagcgaaga gacatggacc agaccatcaa tgaacaagtc gtccagacgg acgaagaagc
    23461 caaagccagt gacgctggaa attacgcctt taaagacgtt acctacctgg tcgagcatga
    23521 agtcacactt cagccagtca gctacatcgc gtgttgcttc gtcggcacga cgttccgcca
    23581 tcgaacagtg ctgacccagt tgcagcatct cttccatcga ataatggtag ccgccggttt
    23641 cagtggtgtt gccctgatgc ccctgctctt tcgccagcag atatttaatg gcgcggtgca
    23701 gcgtcaggtc aggataacga cgaatcggcg aagtaaagtg cgcataggac tgcaatgcca
    23761 gaccgaagtg accacggttt tccggatcgt aaatcgcctg tttcatcgag cgcagcagca
    23821 tagtttgcag catttctgca tcaggacgat cggcaaccga ttccagtaac tctgcatagt
    23881 cgcgcggttc cggcttgtta ccgcccggca attccagccc cagctccgcc agcactgaac
    23941 ggaaagaggt aatcgcttcg gtgctcggct tgtcgtgaat acggaacagt gccggttctt
    24001 tggctttctc aacgaaacgc gccgccgaga tattcgccag aatcatgcac tcttcaatta
    24061 acttgtgggc gtcgttacgc tgagtctgtt cgatacgttc aatacggcgt tcagcgttga
    24121 aaatgaactt cgcttcttcg ctctcaaatg agatcccacc gcgttcttca cgggctttat
    24181 ccagcacttt atagaggtta tgcaactctt cgagatgctt aaccagcggg gcgtactgct
    24241 cgcgcagatc ctgatcgccc tgcagaatat gccagacttt ggtgtaggtc agacgtgcgt
    24301 gagagctcat taccgcttca tagaatttgt agccagtcag gcggcctttc gacgaaaccg
    24361 tcatctcgca caccatacac aggcggtcta cctgcgggtt gagcgagcac aggccgttag
    24421 agagcacttc cggcagcatc gggataacct gcgaagggaa gtacaccgac gtaccacggt
    24481 tacgcgcttc tctgtccagc ggtgttggcg gacgcacata gtagctgacg tcggcaatcg
    24541 cgacccataa acgccagccg ccgccgcgtt ttttctcaca gtaaactgca tcgtcaaagt
    24601 cacgggcgtc ttcgccatca atggtgacca gcggtaaatc gcgcaaatcg acacggcccg
    24661 cttttgcttc ttccggcact tcttctttca gcccggcaac ttgttgctca acagcctgcg
    24721 gccagatata cggaatttca tgggtacgca gagcgatatc aaccgccatg ccggtgccca
    24781 tattgtcgcc aagtacttcg acgattttac ccaccgcttt ggtacggcga gtcgggcgtt
    24841 gggtcagttc aaccaccacc acaaagccca tccgcgcgcc catgatctga tcgggcggga
    24901 ttaagatatc gaagctcaaa cgactgtcgt caggaaccac aaagccgacg cccgcttcgg
    24961 taaagtagcg accaacaatc tggctggttt ttggcaccag tacgcggaca atacgcgctt
    25021 cacgacgacc tttacggtca gcgcccagcg gctgcgccag cacctgatcg ccatgaatgc
    25081 aggttttcat ctgctcgctg gagagataca aatcatcttt acgcccttca acccgcagaa
    25141 agccgtagcc atcacggtgg ccaataacgg tacctttcac caggtcgagg cgttccggca
    25201 gcgcatagca ctgacgacga gtgaagacca gttgaccatc gcgctccatc gcgcgcaggc
    25261 ggcgacgcag gccttcaagc tgctcttcgc cttcaatgtg cagttctacc gccagctcat
    25321 cacggctggc cggtttttca cgtttggtta aatgttcgag gataaattcc cgactaggga
    25381 tgggattcgc gtatttttca gcttcgcgtt cctggaaagg atcttgtgac atctcggttc
    25441 ctccgttgtc atctctgatg aagattttcg tcactccacc agcaataatt tataaagcgg
    25501 ttgattctct tcaaccaaat cggcaagcgt gtagttatcc agttccgtaa gaaaactttg
    25561 cacggcctta gaaagtgcct gtttcaaccg acaggcaggt gtaatgtggc aaaactcact
    25621 gctgcaattc accagcgata agggctccag ctcgcgcacc acatcaccaa tacgtatcgc
    25681 actcgccggt ttacccagac gaatgccgcc attttttcca cgaacagcag tcacgtagcc
    25741 ggcacgacta agttgattga ttattttgac catatgatta cgggagacgc cgtagacgtc
    25801 agtcacttca gaaatactgg tcatccgccc ttctggcaat gacgccatgt agatcagcgc
    25861 acgtaatccg taatcagtga aactcgttaa ctgcacatca acctcaaaag ggaaatcggg
    25921 aaaaaataca tttatattga tgataaacca gccacaagct gtgtcgctaa tttatttcag
    25981 ttcggggaag gaaaaaagcg aggatttaac actgtgtcgg atagcgtaat ggcttacccg
    26041 acacagcaat gtatcacact gttagcccgg caggcaaaat atctgccagg cgtaccagaa
    26101 ttacgcgtcg aacgggtcgc gcagaatcat ggtttcagta cgatccggac cggtagagat
    26161 gatatcgatc ggcacaccgg tcagctcttc aatacgcttg atgtagttca gcgccgcctg
    26221 cggcaggccg ctacgatctt tcacgccgaa ggtggattca gaccagcccg gcatggtttc
    26281 gtaaatcggc tctacacctt tccagtcgtc agctgccagc ggagtggtag tcacttcacg
    26341 accatccggc atacggtaag ccacgcagag tttcacctct ttcaggccat ccagaacgtc
    26401 cagcttggtc aggcagaagc cagacaggga gttcagctgt accgcacgac gaacggcaac
    26461 ggtgtccagc cagccggtac gacgacgacg acccgtagtt gcgccgaatt cgttaccctg
    26521 cttgcagagg aactcgccag tttcatcaaa cagttcagtc gggaacggac ctgcacccac
    26581 acgagtggag taagctttga ggatacccag aacgtaatca acataacgtg ggcccaggcc
    26641 ggaaccggtc gccacgccac cagcagtggt gttggaagaa gttacgtacg gataagtacc
    26701 gtggtcgata tccagcagcg taccctgcgc accttcaaac atgacgaaat cgccacgctg
    26761 acgcgcctgg tcgagcagat cagaaacgtc aacaaccata gaagtcagga tgtcggcaac
    26821 agccatcgta tcatccagaa ctttctggta atcaaccgct tcagctttgt agtagttaac
    26881 caactggaag ttgtgatatt ccatcacttc tttcagtttt tcagcgaagg tttctttgtc
    26941 gaaaaggtcg ccaacacgca gaccgcgacg tgccacttta tcttcataag caggcccgat
    27001 accacgaccg gtggtgccga tcgctttcgc gccacgcgct ttctcacgcg cgttatccag
    27061 cgcaacgtga taatcaagga tcagcggaca tgcttcagac agcagcagac gctcacgaac
    27121 ggggatgcca cggtcttcca gttctttcat ctctttcatc agcgcagccg gagacagcac
    27181 aacaccgtta ccgatgatgc tggttacatt ctcgcggaga atacctgatg gaataagatg
    27241 gagaacggtt ttttcaccgt tgattacgag agtatggcct gcgttgtgac cgccctggta
    27301 gcgtacaaca tatttagccc gttcagtcag aagatcgacg atcttacctt taccttcgtc
    27361 accccattgg gtgcccagta cgacgacgtt gttacccatt tttcaaaatc accgtttgct
    27421 taaaaatgga ttctaccatc gctttttcag agttacagca ctttttgcac tcaaaaatga
    27481 cctcgatcag tctgtttttt gttcagccaa tcgttttcct caacatgtag tagaccacaa
    27541 cgcccgcaac cacaagtcca ccgccaaaac gacgtaaaat attatcgggc aaattggtca
    27601 tcgcagagat catcttcttc catgccttcg ggtaaagcat cggccctaaa ccttccagta
    27661 ccaaaaccag ggcaagcgcc agccagattg tcgaattcat tcattatcct tatagaaaaa
    27721 gaaaaccacc gacatccctg aggatgcggt ggctttattg acctgtaccg cagtcgttat
    27781 attaacgcgt tgcggaggtc ggcgtcttca tgtagcggaa gaaatcgctg tccgggctca
    27841 tgaccatcac gtcctgattg ccagagaagc tgttctcata agcacgcagg ctacggatga
    27901 atgcgtagaa gtccggatct ttactgaatg catcagcaaa cagtttggct gcttcggcat
    27961 cgccttcacc gcgcatgatg cggccctgac gctcagcttc tgccagcgtt ctggtcactt
    28021 catagtcggc agttgcacgc agtttttccg cttcttcctg accttgtgaa cggtgacgac
    28081 gcgctaccgc ttcacgctcg gcgcgcatac ggttgtagat cgcttcagac acttcggtcg
    28141 gcaggttgat ctgcttaatt cgcacatcga caacttcaat acccagcgcc gccatactgt
    28201 tcgggttgat gaccggaact ttgcccttcg tctcagccgt tacgcgctct gccgcttcgg
    28261 caatggcgtt atctgccgcc ggggtagtaa cttcatcttc tgtacccgca gaaccggagt
    28321 tcagcgcgtc acgtacttcg agggtcagac gaccacggga atcggtgacg atgtctttca
    28381 cgtccaggcg accaatttca gaacgcagac ggtcagagaa cttacgtttc aacagcactt
    28441 ccgcttgcga aatgtcgcca ccgcccgttg ccaggtagta acggctgaaa tcgctgatgc
    28501 gccatttgat gtaagagtcg acgatcaggt ctttcttctc tttggtcaca aagcggtcgg
    28561 cctggttgtc catggtctga atacgtgcgt cgagcatttt caccgtttca atgaacggta
    28621 tcttgaaatg cagacccggc tcataaacca gaggtttgtt gtcatcgtca cgcagtacct
    28681 taccaaaacg cagcgtaata ccgcgctcac cttctttgac gacaaagaca gacatgtaaa
    28741 gcactaccag cacgatgatg ataatcgcga taactgactt acgcatcgtt attccccctg
    28801 acgctggtag tcgttacgct gcgcgttggc gcggcgttgg tccataatat cgccctgact
    28861 ggtggacgac gtgttgcttg ctccactggt tgtggaggaa gaggctggcg gcagacgcag
    28921 cagattgctg gcaccgttat cgctcttcgc cgcaggggcg ttaccacctt tcagcatctg
    28981 gtctaacggc agaaccatca ggttgccacc tttgtcgtta accagcactt tgcgggtgtt
    29041 gcccaacact ttttccatcg tctcgatata cagacgctcg cgagtaattt ccggcgcagc
    29101 tttatattcc ggcagaagtt tagcaaagcg cgccacttca ccctgagctt ccaggatggt
    29161 ctgggccttg tacgcacgcg cctcttcgag gatacgttgc gcctgaccgt tcgcacgcgg
    29221 ctgaacttcg ttggtatacg cttctgcttc acgaatgtat tgctgttcgt tttcacgcgc
    29281 ggcaatcgca tcgtcaaacg ccgcttttac ttcttccggc ggacgagctg cctggaagtt
    29341 gacgtccagc agagtgatac ccatatcata cggacgaatc gtctcttcca gttcgcgctg
    29401 agtatcgcta cgaatcacgg tacgaccttc cgtcagaatg cggtccatgg tgtatttacc
    29461 gataaccccg cgcagggcgc tgtcggtagc ctgacgcaga ttgtcatccg ggctggtcac
    29521 gctatacaga tatttttccg gatcggtgac gcggtactgc acgttcatct caacgcgcac
    29581 tacgttctcg tccgacgtca gcatcacacc agaagcggcc agttcacgca cggcttccac
    29641 gttcaccggt ttgacttcgt cgataaacgt cggtttccag ttcagacccg gctcaaccag
    29701 atggctgaat ttaccaaagc gtgttaccac gccgcgttcg gcttctttaa tggtatagaa
    29761 accactggcc gcccagataa tgacaatcgc tgccgctgcg atggtaacga cgcgaccgcc
    29821 aagctgcggg cgcgggcctt gcgatgaact gccaccgcca gatccggtgc ctttaccgcc
    29881 gcccagacca ccgagctttt tgctcagttt gcggaagata tcatctaaat caggtggccc
    29941 ttgatcgcga ccgcctttgt ttccatttcc ctcagagttg ccgccaggtt tgctgcttcc
    30001 ccacgggtcg cggtcttgtc cgttattacc gggctgattc cacgccatgt ttgtgctcca
    30061 tatttgttat gcggtgatcc ctgttgtctt tcgacgccag aggatatgac tccacgcttc
    30121 agacgctacg ccgttagatc aggtaatcga tcaacgccgg ttcttgttta cagaggcgac
    30181 gccagtcaac gatcggcata cgaacttgca gacttacgct gccgtcctcc tccatccact
    30241 ctttttctat tgcctgaagc tgataaaaac gacttctcag acgcccttcc tgcggtggca
    30301 gacgcaatgt atgctgcgcc acctcgccgg aaagccgctc cgtcaaagcc tgaaaaagct
    30361 gtggtatccc cgctccggtc tgtgcggaaa gccagacacg gatcggtttg ttctcttcgt
    30421 cccgatcaat acgcggttcg aaatcttcca gcatatcgat cttgttcatc accagcaggg
    30481 ttgggatctc gtgagcgtcg atctcttcaa gaaccgtatt caccgcttcg atgttttctt
    30541 gtacacgtac atccgccgca tcaatgacgt gcagcagcaa tgtggcttgc cgcgtctctt
    30601 gtaacgtggc tttaaatgcc gccaccagat cgtgcggcag gtggcgaata aaccctacgg
    30661 tatctgcaag tacggtttca ccgacatctg caacgtcaat acgccgcaac gtcgggtcga
    30721 gggtggcaaa caactggtct gccgcgtaga cccgcgcttc ggtgatgcga ttgaaaaggg
    30781 tagatttacc ggcgttggta tatcccacca gcgaaacagt aggaacgtcg gctttgatac
    30841 gcgattgccg cccctgctca cgctgctttt caactctttc caggcgcgac tgtatctgca
    30901 cgatgcgatt acgcaacaaa cgacggtcgg tttcgagctg ggtttcaccc ggaccacgca
    30961 aacctatccc gcctttctgt ctttcaaggt gggtccagcc acgcaccagg cgcgtagcca
    31021 gatggcgcaa ctgcgccagc tcaacctgca acttaccctc atgggtacgc gcacgttggg
    31081 cgaaaatatc taaaataagg ccggtgcggt cgatgacacg acactcgcac aaacgctcca
    31141 ggttacgctc ctgcgccggg ctcagggcat ggtcaaaaag aacgaccgaa gctcccgtcg
    31201 cttttacggc ttccgcaatt tcaactgctt taccttcacc tacaaaatac tttgggtgcg
    31261 gcgctttacg gctaccggta atcacctgca atgcttcgac accggcggaa gagaccagag
    31321 attcaaactc ctggaggtct tccatatctt tgtcttgcgt aaaatagatg tgtaccagta
    31381 ccgcctgctc accagcatca taacggtcaa acaagcgtat aaccctctaa atagatcagc
    31441 ggggaacgca ggatcgctgg ctccccgtgt aaaaaaacag cccgaaacct tattcggttt
    31501 cttcgctgtc ctgttgcgcg gaagtattct gcgcgctgct accatgatgg tagttactgc
    31561 tggtaccgcc accggcgttg ttactgtgat gagaaaccgg gcgagacggg acaacagtag
    31621 aaatcgcgtg cttgtaaacc atctgactga ccgtgttttt caacaggatc acgaactgat
    31681 caaaagactc gatttgccct tgcagcttaa taccattcac caaataaata gaaactggaa
    31741 cacgttcccg acgcagtgcg ttcaggaacg gatcttgtaa agattgcccc ttagccattc
    31801 tctcttttcc ttatatgctt atttgtactt tgaacctttc gattctgaaa aaattgcgca
    31861 cgatacgtct caattgtaca cattcagcct gcgatagcac caacaacctg taatacttcg
    31921 tcacgcgctt gttctggttt ttcactgtca agccagtgaa ccccttccca accacgcagc
    31981 caggttatct gccgcttcgc caactgtctc gtggcgcaaa cacctcgata aaccatttca
    32041 tcgtatgaga tttcgccttc aaggtaagac cacatctggc gataacccac gcaacgaatg
    32101 gaaggcaaat ccgtatgcaa atctcctcgg gcaaaaagcg cccggacttc tgcttcaaaa
    32161 cctgaagcca acatctgatg aaaacgctgc tcaattcgtt gatggagcag ttcacggctc
    32221 gccggggcga tggcgaactg atgcacctga tacggtagag cgtctcctga cgtttgcgtc
    32281 agttccgtta aagttttacc cgaaatgaaa aaaacttcca gtgcccggga aagcctttgt
    32341 ggatcatttg gatgaatcct tgctgccgca accggatcga tctcctgaag ttgacgatgc
    32401 aatgactccc aaccttgctc tgccgcctgt tgctcaattc tggcccgcac ttccgggtct
    32461 gccgacggta gcggcgacaa cccttccagc aatgccttga aatacaacat cgtaccgccc
    32521 actaacagtg ggatccgccc cgccgcggtg atatcggcca tttccgccag cgcatcgcgg
    32581 cgaaaatcag cagccgagta agcctgcgac ggatcgcgaa tatccagcaa tcggtgcggc
    32641 gcggcgagta actcttcagc gttcggcttc gccgtcccga tatccatccc tttgtaaata
    32701 agggcagaat caacgcttat caactctact ggtaaaattt tacgcagctc aatggctaac
    32761 gccgttttac cggaggccgt cggccccatc aaaaaaatcg ccttaggcag gctcgcctta
    32821 ctgatatcac tcatctttca gggcttttat cgccggatgt aaatcaacag attgtaacag
    32881 accacccggc ggcgttttca caagttgcgg acataaccgt tccacgtccg ccagcagggt
    32941 tatggcctgt gccattgacc actgcgcatg ttcgctcatc agatttcgtg caatccactg
    33001 cgcaatattg ccaggttcga atacggactg cttcgccagg tagcctatca gttcaggaat
    33061 caagatttgt aaattttgtt ggcgtaaggg taaaggcacg gccctgatgg tcacatgctg
    33121 cgcatcggac tggaaatcaa tacccaattc cgccagggca gactgcgctt tttctaatgc
    33181 cgatttttct tcgccagaaa cttttagccg caacggaata agcagcggtt gggcgcaaac
    33241 tggcgcttca cccggcgtca gttgcgcctg acgcagccaa cgttctgcca ctggcaaggc
    33301 taaaagtgaa atgtttccgt cgcgctccag caaggcacag tcggaatgga cgatagtcag
    33361 tacccgacca aaactctgac tgttcgccgc aagtgcaggt tcctgcggtt ctggcgcttt
    33421 tggtttttgc atcggcgcgg gcgtttgcaa aagctggcga tacacttcac cttgctgttt
    33481 ctggtaacct ggctgcgcat tcggccaggg ggcagccgga cgactgcctg aggcgggcgc
    33541 aggagtgtaa cgcggagcta ctggctcacg aactgccggt tcagcaaaat gattgcgccc
    33601 cgccgccacg cggttttccg gaatagcacg cggtgcaggt tggggttcat cgtccagcgg
    33661 tagcggcgtt tccagttgct gttgtagcac gctcagcacg ccctgataga taaaatcatg
    33721 caccagacgc gactgatgga aacgcacttc gtgtttggca gggtgcacgt tgacgtccac
    33781 ctgatgtggg tcgatctcca gatacaacac aaatgccggt tgctgatcgg cccccagttt
    33841 gtcttcacag gcctggcgga tcgcgtgatt gatcaggcga tcgcgcatca tgcgaccgtt
    33901 cacgtagcaa tactgaattt ctgccagggc gggcgtggtg tgattcggat cggccaccca
    33961 gccgcgcagg gtgagatcgc cgtgttgcca ttcaatcgcc agcgcttgtt cgagaaaagc
    34021 ggtgccgcaa atcgcgccta agcgccgttc tttttgcccg ccttccggca ctgcacggta
    34081 ctgacgcaca attttaccgt tatgcgacag gttgatcgtg acgtcgaaac gcgccagcgc
    34141 aatgcggcga ataatttcat cgatatggtt aaattcggtt ttctcggtgc gcaggaattt
    34201 gcgccgcgcc ggggtgttgt agaacagatc cagcacctcc agcgtcgttc ccaccggatg
    34261 cgccgccggt ttaaccgtca cgtccatatc gcgcccttcg gcataggcct gccaggcttc
    34321 ctgctgttct gcggtgcgtg aagtgagcgt caggcgggaa accgaactga tactcgccag
    34381 cgcctcaccg cgaaagccca ggctgataat ggcttcgaga tcgtccagag aggcgatttt
    34441 actggtggca tgacgagcca gcgccagcgc cagctcatct tttttgatac cgcagccgtt
    34501 atcacgaatg cggataagtt tcgccccgcc acgttcgata tcaatatcga tacgcgtcgc
    34561 acctgcatcg aggctgtttt ccaccagttc tttgactacc gacgcaggtc gctcgaccac
    34621 ctcacctgcg gcaatctggt tcgccagttg tggcggtaag acctgaattg gcatcaatcg
    34681 tccttagttt ggcagcgtac gatctggcgt cgtcaccgta ctggcagttt gtgccgttgc
    34741 accctgcggc gcagattgca tcggatgcgc aaggaaataa ttgcgcagac ctttataaat
    34801 ggcttctgcc agctgttgtt ggtaatcgtc gctcgccagc aaacgttctt cgctgttgtt
    34861 gctgataaaa ccggtttcga ccagtactga tgggatatcc ggcgaacgca gaacgccaag
    34921 gctggcgtgt tctggtcgac gtttatgaat ttcgccaatg cgttgcaact gactgatcat
    34981 actggtcgct acatcatacc ctacccgctg ggaatgaccg aactgtaaat ccagcaccgc
    35041 ctggctcaaa taggggtcag actgactgtt cgccagcaca tcgcccgctc cgcccagtag
    35101 ctccgactgt ttctcatgct gttccagcca gcttgccatc tcgctgtttg cacgacggtt
    35161 agagagcacc catacggaag cgccagtcgc actgcggttt ggtgcggcat cagcgtgaat
    35221 cgacacgagg aaattggcgt tttgcttacg tgccacatcg ctgcgcccca tcaccgaaat
    35281 aaagtaatcc ccgtcacggg ttaaaacgcc tttaaacatt ggatcgtcat tgagcaaagt
    35341 acgtaattta cgtgcgatgg cgatggtgac atttttctcc cgcgtaccac cggggccgat
    35401 agcgccagga tcctgaccgc cgtgtccggc atcaatagcg atgataattt tatcgccagt
    35461 gttagccgtc gcgcgcgctg ccggacgcgt taccgtatta ctgctgataa cacccgtagt
    35521 gcggttactt tccgttttaa acggattgcg cgccggttcg ctgacgcgcg gtgcgccaac
    35581 cgcaggcgtt tcaacgcgtt tcgcaaccac aggcggcgga ggaggcggtg gcggcgcatc
    35641 ggcgttaatc gtaaagacga cagtgtaatt gctgccattc tgccgcttca ccgcttcggt
    35701 tttaccattt tcggtaagat cgaccaccag ccgtagcgtt tgtgcatctt taggcgttcc
    35761 agagcgaatc gccttcacca gattattgcc gctgaacaac aacggcagtc cctgaatcac
    35821 gcccgtttgt ttgatatcga gcgccacgat gcgtttgctt tgatggctaa acgcataatc
    35881 aggatcgcca ataaaactca acgttatccg cgcctgttgg ttgccgttag aaacctgaat
    35941 atcagagagc gtcgcggcac ccacctgcgc gcacagcagc agcaacgtcg ctaccaacca
    36001 atttctgatg cgatacatca tcccgccacc tttcaaaggt taaccggcta aacgcgccag
    36061 caacaattca cccgcagagg aaaccgcact cacgcgcgcc tcacggcctt gtgcctgata
    36121 atcaatgtgt atttcgacat ccgggtcagg aagaacacct gtaccttgtt gtggccactc
    36181 caccaggcag atggcatcgt tggcaaaata atcgcggatc cccataaact ccagctcctc
    36241 gggatcggca aggcggtaca aatcaaagtg ataaaccatt aaattgtcga gcgtataggg
    36301 ttcgaccagc gtataagtgg ggcttttgac attaccctga tgacccaggg cttgtaaaaa
    36361 gccccggcta aaggtggttt tacctgcgcc taaatcgcca tacagataga ttacggttgc
    36421 gccatcgcag gctttcgcta cccgctcgcc caggtctaat gttgcctgct catcagggag
    36481 cggaattact cgattcatca tggtttttat cagtcacttc cgggttaaca atacgctgta
    36541 gcgtggaaaa gagatcggtt gccagcatcc cgcgcgttcc aaaacgcgcc gccagtacgt
    36601 cagctgccgc accgtgcgcg acacagcccg cacaggctgc atcataaggg ctcatttttt
    36661 gcccaagcaa tgcgccaata ataccagaga gcacatcgcc catgccgccg ctcgccatgc
    36721 ctgcatttcc gacatcaata atgcctaaag cgtcagaatg ggcggcgacc acggttccgg
    36781 cacctttcag caccgctacg ccgccataac gttgtaccag acgttgggcg caatgtaagc
    36841 ggtcactttc aatttcagcg acggaacagc ctaacaaccg tgcggcctcg ccaggatgcg
    36901 gcgtgatcac gcgattgtga cgcttatcgg gattgattgc cagcaggttc aatgcatcgg
    36961 catcccacaa catcggtttg cgaaaattct caactttttg cagggctttt ttcccccact
    37021 cttgctggcc cagaccggga ccaatcacca ccacatcggc ccattccagg ctttcggtaa
    37081 gagagtccat agtcagttca tgcaccatca attctggtcg tgcagtcagc agcggcgcaa
    37141 tgttctcact gcgggtcagt actcggacta aaccagcacc agcacgcagc gccgcttccc
    37201 ccgtcatacg aatagccccc gccgtgccgt gatcgccacc gataatcacc agccgcccgt
    37261 gatcgccttt atgcgaagtc gggcgacgcg gtttcagcca gtgagaaagt tgttctgccg
    37321 aaaaccgctg aattttcgtc tcctgacctg ccagccagct atccagcccc agtgagtcaa
    37381 aatgcagttg tcctgtaaca tcccgcgctt ttccagtgag caagcctggt ttcagcgcaa
    37441 taaaagtgat ggtgtgatcg gcgttgatca ccgcgcctgg cgtagcgcca gtttcagcca
    37501 gcaggccgga agggatatca accgccacaa tcggcgcagg atgggaatta gcgtggtcga
    37561 ttaactggct aatggattca cgaggcgctt gctgtaatcc ggtaccgagc agcgcatcaa
    37621 caatcagatc taccgattcg ggccagacaa tattcgaagc atggatctcg cctcccgcgt
    37681 ttaaccatgc ttcgcgtgcc agcgcggcct cttccggcaa cggtttgtca ctttcctggg
    37741 ccagcagcgt gacatcaata ccgaccgctt tggccagtcg cgcgaccacg tagccatcgc
    37801 cgccgttatt accatgaccg cacagcacca gccagtggcg ggcgtcagga taagccgaac
    37861 gacacacctg gaatgcggct tcgccagcgc gaagcatcag ctcatagagt gtgagcccca
    37921 gcgcatctac cgcctcgcgt tctccgcggc ggatatcgtc ggcgtgccag acggtgtgtg
    37981 gtatacttac ggggtttttc ttcattgtat ggtccgtcat gtcagagccc ctcgatctca
    38041 atcagttagc gcaaaaaatt aaacagtggg ggctggaact gggctttcag caggtaggta
    38101 ttaccgatac cgatctcagc gcgtccgagc ccaaactgca agcatggctg gacaaacaat
    38161 accacggcga aatggactgg atggcgcgtc acggtatgct gcgtgctcgt cctcacgaat
    38221 tattgcccgg tacgctgcgc gtgatcagcg tgcggatgaa ttaccttcct gctaacgccg
    38281 catttgccag cacgctgaaa aaccccaaac tcggctatgt tagccgttat gcgctgggcc
    38341 gtgactatca caaacttctg cgcaaccgac tcaaaaagct gggcgagatg attcagcaac
    38401 attgtgtttc gctgaatttt agaccgtttg tcgattctgc gcctattctt gagcgcccgt
    38461 tagctgaaaa agctgggctc ggctggacag gtaagcactc acttatcctc aatcgcgagg
    38521 ccggctcgtt cttcttttta ggcgaattgc tggtcgatat tccgctaccc gtggatcaac
    38581 cagtcgagga aggatgcggt aaatgcgtgg cctgtatgac gatttgcccg accggtgcca
    38641 tcgtcgagcc atataccgtc gatgctcgcc gctgtatctc ttatctcacc atcgaactgg
    38701 aaggggcgat cccggtagag ttgcgaccat taatgggaaa ccgtatttac ggttgcgatg
    38761 actgccagct tatctgcccg tggaatcgct attcgcaact cactacagaa gacgatttca
    38821 gcccgcgtaa gccgctacac gcaccggaac tcattgagtt attcgcctgg agcgaagaga
    38881 agtttttaaa agtcacggaa ggttcggcga ttcgccgtat aggtcacctg cgttggctgc
    38941 gtaatatcgc cgtagcctta ggcaatgccc cctgggatga aacgattttg acagcgctgg
    39001 aaagtcgtaa aggtgagcac ccacttcttg atgagcacat agcgtgggcg attgcgcagc
    39061 aaatcgagag gcgaaatgcg tgcgtggtcg aagtgcaatt accgaaaaaa cagcgtctgg
    39121 ttcgggtgat tgaaaaaggg ttaccgcgtg acgcctgatt catccacagc ttgtgaataa
    39181 aaataaaaac gcattgcaat tcaagccgga aaaaatcgtc aagtgatcga tacgacaaaa
    39241 cgaaatctta atttttaact tatctatcaa taggttaaaa aaatactatt caccgtgcga
    39301 agtttctttg aaattaagat tacggcctga ggctgtggat aactctgttt acaagagttt
    39361 gtcagagaca acaaaaacca gcaataacgc atcgcgtcgc tgtggatatt ttattgagag
    39421 aagaatttgg agcgggaaac gagactcgaa ctcgcgaccc cgaccttggc aaggtcgtgc
    39481 tctaccaact gagctattcc cgcttgggtg gtctgtgctt tacagcactt tcaaattttg
    39541 gagcgggaaa cgagactcga actcgcgacc ccgaccttgg caaggtcgtg ctctaccaac
    39601 tgagctattc ccgcttaatc ttcgtctttc gaactgctac taccttggct gccttcactc
    39661 accttagtca cttacttttg taagctccca aggattcgtt cagttgccgc cttgtcgcaa
    39721 ttcgaaatac tgcgattggg tggtctgtgc tttgcagcac ttttcaaatt ttggagcggg
    39781 aaacgagact cgaactcgcg accccgacct tggcaaggtc gtgctctacc aactgagcta
    39841 ttcccgcgta ctacttaatt ttgctttcgt aattttcgca tttctgcgtc gttacgggag
    39901 gcgcattata cgagaaatct ttccctctgc aaccccccta aaaaattttt tttgagtttt
    39961 tatctcaagt gctgaaatta tcggcaagac gttcaattta gcgtcaaaat cacgccaccg
    40021 caagtggatt acggcgctta cgtgaccagg aaaaaattta cagcttgata aaatgctcgc
    40081 ggtagtaagc cagctccgcc accgattcac ggatatcatc catcgcctga tgcgtcccct
    40141 gcttggtaaa accatcaaga atttccggct tccagcggcg cgccagctct ttcagggtgc
    40201 tgacatcgag ataacggtag tggaagtagg cttccagctc cggcatgtat ttaaacagga
    40261 agcgacggtc ctgaccgatg ctgttaccac aaatcggcga ttttcccgca ggcacccact
    40321 gttttaagaa ttcgagcgtt gccagttcag cttcacgatc gcccatcgtg ctcgctttca
    40381 cgcgctccac cagcccgctg gcggtatggg tgcgcacgtt ccagtcatcc atcagcgcca
    40441 gctgttcatc ggactgatgt actgcaatgg tcggcccttc tgccagaata ttcaggttgg
    40501 catcggtcac cagcgtggca atctcaataa tgcgatcgcg ctcgggatcc agaccggtca
    40561 tctcaagatc gatccaaatc aggttgtttt cattggcact catgctattt tccacccatt
    40621 cacagtgact atattgatcc acaattgcgt gtatcataga tgttttgccc atcaggggcg
    40681 accaggagtc tgtacgattg agtaaaaata aactctccaa aggccagcag cgccgcgtga
    40741 acgccaatca ccagcgtcgt cttaaaacgt ctaaggagaa gcccgactac gacgacaatc
    40801 tgtttggcga gcctgatgaa ggtatcgtca tcagccgctt tggtatgcac gctgatgtgg
    40861 aatccgcaga tggcgacgtt caccgctgca atattcgccg taccatccgt tcgctggtga
    40921 ccggcgaccg cgtagtctgg cgtccgggta aaccggcggc ggaaggcgta aatgtcaaag
    40981 ggatcgtgga agcggtgcat gagcgcactt cagtgttaac gcgcccggac ttctacgatg
    41041 gcgtgaaacc tattgccgcc aacatcgacc agattgtcat tgtctccgcc attttgccgg
    41101 agctgtcgct caatattatc gaccgttacc tggtggcctg cgaaaccttg cagattgagc
    41161 cgattattgt gctcaacaaa atagacctgc tggacgacga aggtatgaag ttcgtcaacg
    41221 agcagatgga catctaccgc aatatcggtt atcgcgtatt gatggtttcc agtcatacac
    41281 aggatgggct gaaaccgctg gaagaggcgt tgaccgggcg catcagcatt tttgccgggc
    41341 agtctggcgt tggcaaatcc agcctgctga acgcgttgct ggggctgcaa aaagagatcc
    41401 tgaccaacga tgtctctgac aactcggggc ttggtcagca caccacgacc gccgctcggc
    41461 tgtatcactt cccgcacggc ggtgatgtga ttgactcccc aggagtgcgt gagttcggcc
    41521 tctggcacct ggagccggaa caaatcactc agggctttgt cgaattccat gactatttag
    41581 gtctgtgtaa atatcgcgat tgcaaacacg ataccgatcc gggctgcgct atccgggaag
    41641 cggttgagga agggaaaatc gcggaaaccc gtttcgaaaa ctatcaccgt attctggaaa
    41701 gcatggcgca ggtaaaaacg cgtaaaaact tttctgatac ggatgactga caattaagct
    41761 aaccgtcgtt agaatcgtcc ccttttttca ggatccggca tgtaggccgg atcaggaacg
    41821 acaaaacaat ggcctggagg ctaccttgtt aaattcattt aaactttcgc tacagtacat
    41881 tctgccgaaa ctatggctta ctcgcctggc gggttggggc gcaagcaagc gggcaggatg
    41941 gctgacaaaa ctggttatcg atctgttcgt taaatactac aaggtcgaca tgaaagaggc
    42001 gcaaaagccg gataccgcca gctaccgcac ctttaacgaa ttctttgtcc gtccgctgcg
    42061 tgacgaagta cgcccaatcg ataccgatcc gaatgtactg gttatgcctg ccgatggcgt
    42121 tatcagccag ttgggtaaaa tcgaagaaga taaaatcctg caagccaaag gccacaacta
    42181 cagcctcgaa gccctgctgg caggcaacta tctgatggcg gacctgttcc gcaacggtac
    42241 gtttgtgacc acttacctct ccccgcgtga ctaccaccgc gtacacatgc cgtgcaacgg
    42301 tattctgcgt gagatgatct acgtgccggg cgatctcttc tccgttaacc atctcaccgc
    42361 tcagaacgtg ccgaatctgt ttgcccgtaa cgaacgcgtg atctgcctgt tcgataccga
    42421 atttggccca atggcgcaga ttctggtcgg ggcgacgatt gtaggcagca ttgagacggt
    42481 ctgggcgggc accattacgc cgccgcgcga aggtatcatc aagcgctgga cctggcctgc
    42541 cggggaaaac gacggttctg tggcactgct gaaaggccag gaaatgggtc gctttaaact
    42601 cggttccacc gttatcaacc tgtttgcacc gggtaaagtg aatctggttg agcaactgga
    42661 aagcctgtct gtcacgaaaa ttggccagcc gctggcagta tctaccgaaa cctttgttac
    42721 gccagacgct gaacccgccc cgcttcctgc tgaagagatc gaagcagaac acgacgccag
    42781 cccattggtt gacgacaaaa aagaccaggt ctaatccatc aaaggaaacg ctgacgtgcg
    42841 cctgattatc acttttctga tggcctggtg cctcagttgg ggggcgtacg ccgcgacggc
    42901 ccccgatagc aaacaaatct cacaagaact ggagcaggca aaagcggcga aacccgcgca
    42961 accggaagtc gtagaggcgc tccagtctgc cttaaatgcg cttgaggaac ggaaaggttc
    43021 ccttgagcgc atcaagcaat atcaagaagt cattgataat tatccgaaac tctccgctac
    43081 tctgcgcgcg caattaaaca acatgcgtga cgagccgcgc agcgtgtcac cgggaatgtc
    43141 taccgacgcg ctgaatcagg aaattctcca ggtcagcagc cagttgctgg ataaaagccg
    43201 tcaggcccag caagagcagg aacgcgcccg cgagattgcc gattcgctga atcaactgcc
    43261 gcaacagcaa accgatgccc gccgtcagtt aaatgaaatc gagcgccgcc tggggacgct
    43321 taccggcaat tccccgctca atcaggcaca aaatttcgcg ttgcagtctg actccgcacg
    43381 cctgaaagcg ctcgtcgatg aactggaact ggcgcagctg tccgccaata accgccagga
    43441 attagcgcgc ttacgctcag agctggcgga aaaagagagc cagcaactgg atgcatattt
    43501 gcaggcctta cgtaatcagt tgaacagcca acgtcagctt gaggcggagc gggcgctgga
    43561 aagtaccgaa ttactggcag aaaacagtgc cgatttgccg aaagatatcg tcgcgcaatt
    43621 caaaattaac cgcgaactat cggcagcgct gaatcaacag gcgcagcgga tggatctcgt
    43681 tgcctcgcaa cagcgtcagg ctgccagcca gacgttacag gtccggcagg cgttgaatac
    43741 gctgcgtgaa cagtcgcaat ggctgggctc gtccaacctg ctcggcgaag cgttgcgggc
    43801 gcaggtggca cggctgccgg aaatgccaaa accacaacag cttgataccg aaatggcgca
    43861 gttgcgtgtg caacggttac gttatgagga tctgcttaat aaacagccgc agctaaggca
    43921 aattcatcag gccgacggtc agccgctgac cgccgagcaa aaccgtattc tggaagcaca
    43981 gctacgcact cagcgtgagt tgctgaactc attgttgcag ggtggcgaca cgctactact
    44041 ggaactgacc aagctgaaag tctccaacgg gcaactggag gatgcgctga aagaggtgaa
    44101 tgaagcgacg caccgctatc tgttctggac ctctgacgtg cgcccgatga ccatcgcctg
    44161 gccgctggaa atcgcccagg atctgcgtcg tctcatttcg ctggacacct tcagtcagtt
    44221 gggcaaagcc agtgtgatga tgctgaccag taaagagaca attttaccgc tgtttggcgc
    44281 gttgattctg gtcggttgca gtatttactc acgccgctat ttcacccgtt ttcttgaacg
    44341 ttcggcggcg aaagtcggca aagtgactca ggatcacttc tggctgacgt tacgcactct
    44401 tttctggtcg attctcgtcg cgtcaccgct accggtgctg tggatgacgc tgggttacgg
    44461 cttgcgcgag gcgtggcctt atccgctggc ggtcgcgatt ggcgatggtg taacggccac
    44521 cgtgccgctg ctgtgggtag tgatgatttg cgctaccttt gcccgcccga acggcttgtt
    44581 tatcgctcat tttggctggc cgcgcgaacg cgtttcccgt gggatgcgct actacctgat
    44641 gagcatcggg cttattgtgc cactgattat ggcgctgatg atgttcgata acctcgacga
    44701 ccgcgaattt tccggttcgc tgggacggct ttgctttatc ctcatttgcg gtgcgctggc
    44761 ggtggtcacc ctcagcctga aaaaggccgg gatcccgctg tatctcaaca aagaaggcag
    44821 cggcgacaac attaccaacc atatgctgtg gaacatgatg attagcgcac cgctggtcgc
    44881 tattctggcg tcggcggtgg gttatctggc aacggcgcag gcactgttag cgaggcttga
    44941 aacctcggtt gccatctggt tcctgctact ggtggtttat cacgttatcc gccgctggat
    45001 gctgatccag cgccgcaggc tggcgtttga ccgggcgaag catcgccggg cagagatgtt
    45061 agcgcaacgt gcgcgtggcg aagaggaagc gcatcatcac agtagcccgg aaggagcaat
    45121 tgaagtcgat gaaagcgaag tcgatctcga tgccatcagt gcgcaatcct tgcggctggt
    45181 gcgctcaatt ttgatgttga tcgccctgct ttcggtcatt gtgctgtggt cagaaatcca
    45241 ttccgctttc ggcttcctcg aaaatatttc gctgtgggat gtcacttcca cggtacaggg
    45301 cgtagaaagc ctggagccga ttaccctcgg tgcggtactg attgccattc tggtgtttat
    45361 catcaccacg cagctggtgc gcaacctgcc cgcgctgctg gaactggcga ttctgcagca
    45421 cctggattta acgccgggta cgggctacgc catcaccacc atcaccaaat atctgctgat
    45481 gctgattggc gggctggtcg gcttctcgat gattggtatt gagtggtcga aattgcagtg
    45541 gctggtcgcc gcgctcggtg ttggtctcgg ttttggtttg caggaaattt tcgccaactt
    45601 tatctctggc ctgattatcc tgttcgaaaa accgattcgc attggcgata cggtaacgat
    45661 ccgcgatctt accggtagtg tgacgaaaat taacacccgc gccaccacca tcagcgactg
    45721 ggaccgcaaa gaaattatcg tgccgaacaa ggcgtttatt accgagcagt ttatcaactg
    45781 gtcgctctct gactcggtca cgcgcgtggt gttgacgatt ccggcccctg ccgatgccaa
    45841 cagtgaagaa gtgacggaaa tcctgctcac cgcagcgcgt cgctgctcgc tggtgatcga
    45901 caacccggca ccggaagtct tcctggtgga tctgcaacag gggattcaga ttttcgagct
    45961 gcgtatttac gccgcagaaa tgggtcaccg catgccgcta cgccatgaga ttcaccagct
    46021 aattctggca ggcttccatg cccacggtat cgatatgcca ttcccgccct tccagatgcg
    46081 tctggaaagc ctgaatggta aacaaacggg gagaacatta acttctgcgg gcaaaggtcg
    46141 tcaggcggga agtttgtaag gtctttgtgg ccggatgaac ccatccggct tataagcaaa
    46201 actgatagcg cagaaaaaac tgccacagcc aatagccgaa aatagccagc gcggtggcgt
    46261 acataaacca gccgcgtttt tttcgccaca aagcgaacca gagaaaaggg aaaaagagcg
    46321 gtaacgtcat tatcacaccg acatcacggg tatcgtcatc ttcgagatac atcagcacat
    46381 cgcagacgtt attaatcccg cgtccatcaa tcatccactc tttgtccagc gcggaagtga
    46441 tggttatcca gaggaacgcc acaataaacc agatgcagca caagataaac atgcgcgcac
    46501 tcatgagata atcggtcgct taatttcgct atagcgcata cgaaacaaat cgccgtatcc
    46561 cactaattct ccgcgaaaaa ccagacatct aataagataa gcgcaataga tatccggcag
    46621 gtttccccac cgcggaccat cgcgctcttt gagggtatta atagtgttac ccaccaccat
    46681 cgctactttg cgggtgtggc gggtatcgac aaaagagagg accgtttgct ctatttccgc
    46741 cacatccccg gcagaaaagt gcagctccct gatttcatct tcggttatca ctggatcccg
    46801 actagcgtcg tccataacgt cttcctttcc agacaactca tcgccggaga gacgatgagt
    46861 tgcaggattt catatgatca gtcgagagct aactcaggtt tgttcgccat gcgactgcaa
    46921 tagttctggt aaatcgccat cgctaacagc gagaagaaga cagggccgcc aatcatccac
    46981 aatgtgctgt cccagtctcc agcttcaacc acaggttgaa tgatggtgaa gacgttcgca
    47041 aatgtcacca ccagtacgac aaccactgtt gcaatcattg ccgacatatg cgttttgaaa
    47101 atcacaaacg gtctgtcgag atcctgacgt gctttaaaaa atgggaaagc cagcgcgagg
    47161 aacaggtaag gaagcgtcat agacacgttc gccatcagcg tcagcttgtt aaagaacgcc
    47221 gatgcggtac cgccaccaaa cgaaaccagc agaatgaaga tagtaaccaa cccgcactgc
    47281 atccacatgg caatagacgg catccccatc gtattcaggc gcgtcatcgg ttccggccac
    47341 aatgctttcg gcgttccctg gatgatggct ttcagcggtg aatagcaaag cgtaaagaac
    47401 gcaccggtat aagcgaggaa catcgacagt ccggtaatac gcgcaaacca tacgcccagc
    47461 gacaatgacg cttcaggtga aaaatgcagt gcgttaccca gcgtcacccc gaggctcttc
    47521 atcagcacat aggtaatatt gccgaggtta acggaaccat tacttaatac ctgctgccag
    47581 tttgtgctga cgccccataa aaatattgcc agcgaataac cgattgaaat aacaatagcg
    47641 gcaaaaacaa tacctttggc aaagttcttt tctggatttt ccgttttatc gaccagacca
    47701 cccaccgctt caattccgcc ataggcaaaa atagcaaata ccacaaacga tagcattgcc
    47761 agaccggact gataacctgg gttcggtgat gcaaggaaat taatatcctg cgcgaaatgc
    47821 ccaccattta ataacaaaat agtaatgctt actaacagca atactaaatt cagacacatt
    47881 actgcaatac cgcccaccgc agtaatgcgg gcaattttat taatcccctt tgaagcaacg
    47941 acggtgacca gaatcatcca tgccactgcc agcagaccaa ccacctgcgt aggctccagt
    48001 ccggcaatac gccagtgctg ggtcatgtcg ctaccataga ggaatgttga gaacggtacc
    48061 caaactttcg ctgaggtact caccatccag atgatataag aggaaaacca cataaatgta
    48121 ccaatgaagg caaaacgtgg tccgacacta ttattcatcc aggaatagat accaccttct
    48181 tctttgcgat aagccgctcc catttcagcc atcattaagg cgaatggaat aaagaataac
    48241 aatgcagaaa atatataaaa gggaatcgca ctataaccca ttaagtaata agccgatggg
    48301 ctattggcaa atccaaatac ggaagtaaag atcatcaata tgagtcctat cagactcatc
    48361 ttttttatcg tgtgaggcat gaaaccatcc ttcatttcag cgcgtgtata aaacgcgccc
    48421 actgaactgc actgcccaac attgagcggg cagcgtaaaa accaatacag aattgatacc
    48481 tggatgatat caaatatcca gtcttaaatt gtggccttta gcggaaaaat gaaaacatat
    48541 gctacagaat ggcgcttatc acgccattct tcgcggttaa ttcagtaatt tttcagaatt
    48601 atgcccggtc aacgctaaag gcgatgactt cagccagtgt ctccgcgccc agcgccaaca
    48661 tcaccagacg atcaacgcct aatgccacgc cggaacagtc aggcataccg actttcaagg
    48721 cttcaatcag attctggtca atggggtgct gcggcaaacc gcgcgccgcg cgcttacggt
    48781 tatcttgttc aaagcgttgt tgctgctcac gggcatccgt caattcatgg aaaccattcg
    48841 ccagctcaat acctttataa taaacctcaa agcgttcagc gacccgatga tcttcggtac
    48901 tgatttgcgc cagtgatgcc tggctggctg gaaagtggta cacaaaggtc ggtttttctt
    48961 tgccaatatt tggctctacg ccaaaggtaa acagcaattg tagcaacgtg tcgcggtctt
    49021 cttcggtatc tgcaacgttg ctcaaatcca gtttcgctgc cacttcccgc agttgcgttt
    49081 tgtcggcaga gagcgggtca atttccagat aacgcaagaa agcttgttga taagaaaggc
    49141 tttctgctgc cgggcagtcc agcacttgtt gtaagagatc gtccacctcg ttcatcaacc
    49201 ggtacatatc atagtgcggt cgataccact caagcatagt gaactcaggg ttgtgataac
    49261 gccccatctc ttcattacgg aagctgcggc acagctggaa taccggccca caaccagcaa
    49321 ccagcaggcg tttcatatgg tattcagggc tggtcattaa ccagagattc atcccctgcg
    49381 aatgcccggg gccaacgaaa cgtgtctcaa acgggaccaa atgaatatcg gttaccgtcg
    49441 cctggctcat acaaggcgtc tccacctcca gcactccacg atcggcaaag aaacgacgga
    49501 tctccgccat aatcgccgcg cgttttaata agttaggaat ggatgcgctc ggctgccagg
    49561 atgccgtttc gctcatagtt aaatctccag tttttgacaa gggcacgaag tctactcgca
    49621 acgcgacggc gagacaaatt ttacgcagga atcaaacaac ggtgggcagt gactaaaaaa
    49681 agcacgatct gatggtttag taattaaatt aatcatcttc agtgataatt tagccctctt
    49741 gcgcactaaa aaaatcgatc tcgtcaaatt tcagacttat ccatcagact atactgttgt
    49801 acctataaag gagcagtgga atagcgttcg cagaccgtaa ctttcaggca cttaccctga
    49861 agtacggggc tgtgggataa aaacaatctg gaggaatgtc gtgcaaacct ttcaagccga
    49921 tcttgccatt gtaggcgccg gtggcgcggg attacgtgct gcaattgctg ccgcgcaggc
    49981 aaatccaaat gcaaaaatcg cactaatctc aaaagtatac ccgatgcgta gccataccgt
    50041 tgctgcagaa gggggctccg ccgctgtcgc gcaggatcat gacagcttcg aatatcactt
    50101 tcacgataca gtagcgggtg gcgactggtt gtgtgagcag gatgtcgtgg attatttcgt
    50161 ccaccactgc ccaaccgaaa tgacccaact ggaactgtgg ggatgcccat ggagccgtcg
    50221 cccggatggt agcgtcaacg tacgtcgctt cggcggcatg aaaatcgagc gtacctggtt
    50281 cgccgccgat aagaccggct tccatatgct gcacacgctg ttccagacct ctctgcaatt
    50341 cccgcagatc cagcgttttg acgaacattt cgtgctggat attctggttg atgatggtca
    50401 tgttcgcggc ctggtagcaa tgaacatgat ggaaggcacg ctggtgcaga tccgtgctaa
    50461 cgcggtcgtt atggctaccg gcggtgcggg tcgcgtttat cgttacaaca ccaacggcgg
    50521 catcgttacc ggtgacggta tgggtatggc gctaagccac ggcgttccgc tgcgtgacat
    50581 ggaattcgtt cagtatcacc caaccggtct accaggttcc ggtatcctga tgaccgaagg
    50641 ctgccgcggt gaaggtggta ttctggtcaa caaaaatggc taccgttatc tgcaagatta
    50701 cggcatgggc ccggaaactc cgctgggcga gccgaaaaac aaatatatgg aactgggtcc
    50761 acgcgacaaa gtttctcagg ccttctggca cgaatggcgt aaaggcaaca ccatctccac
    50821 gccacgtggc gatgtggttt atctcgacct gcgtcacctc ggcgagaaaa aactgcatga
    50881 acgtctgccg ttcatctgcg aactggcgaa agcgtacgtt ggcgtcgatc cggttaaaga
    50941 accgattccg gtacgtccga ccgcacacta caccatgggt ggtatcgaaa ccgatcagaa
    51001 ctgtgaaacc cgcattaaag gtctgttcgc cgtgggtgaa tgttcctctg ttggtctgca
    51061 cggtgcgaac cgtctgggct ccaactcgct ggcggaactg gtggtcttcg gtcgtctggc
    51121 cggtgagcaa gctatggaac gcgcatctac tgccggtaat ggcaacgatg cagcaatcaa
    51181 tgctcaggtt gctggcgttg aacagcgcct gaaagatctg gttaaccagg atggcggcga
    51241 aaactgggct aagatccgcg acgaaatggg catggccatg gaagaaggct gcggtatcta
    51301 ccgtacgcca gaactgatgc agaaaaccat cgacaagctg gcagaattgc aggaacgctt
    51361 caagcgcgtg cgcatcaccg acacttccag cgtgttcaac accgacctgc tctacaccat
    51421 tgaactgggt cacggtctga acgttgctga atgtatggcg cactccgcaa tggcacgtaa
    51481 agagtctcgc ggcgcgcacc agcgtctgga cgaaggttgc accgagcgtg acgacgtcaa
    51541 cttcctcaaa cacaccctcg ccttccgcga tgctgatggc acgactcgcc tggagtacag
    51601 cgacgtgaag attactacgc tgccgccagc taaacgcgtt tacggtggcg aagcggatgc
    51661 agccgataag gcggaagcag ccaataagaa ggagaaggcg aatggctgag atgaaaaacc
    51721 tgaaaattga ggtggtgcgc tataacccgg aagtcgatac cgcaccgcat agcgcattct
    51781 atgaagtgcc ttatgacgca actacctcat tactggatgc gctgggctac atcaaagaca
    51841 acctggcacc ggacttgagc taccgctggt cctgccgtat ggcgatttgt ggttcctgcg
    51901 gcatgatggt taacaacgtg ccaaaactgg catgtaaaac cttcctgcgt gattacaccg
    51961 acggtatgaa ggttgaagcg ttagctaact tcccgattga acgcgatctg gtggtcgata
    52021 tgacccactt catcgaaagt ctggaagcga tcaaaccgta catcatcggc aactcccgca
    52081 ccgcggatca gggtactaac atccagaccc cggcgcagat ggcgaagtat caccagttct
    52141 ccggttgcat caactgtggt ctgtgctacg ccgcgtgccc gcagtttggc ctgaacccag
    52201 agttcatcgg tccggctgcc attacgctgg cgcatcgtta taacgaagat agccgcgacc
    52261 acggtaagaa ggagcgtatg gcgcagttga acagccagaa cggcgtatgg agctgtactt
    52321 tcgtgggcta ctgctccgaa gtctgcccga aacacgtcga tccggctgcg gccattcagc
    52381 agggcaaagt agaaagttcg aaagactttc ttatcgcgac cctgaaacca cgctaaggag
    52441 tgcaacatga cgactaaacg taaaccgtat gtacggccaa tgacgtccac ctggtggaaa
    52501 aaattgccgt tttatcgctt ttacatgctg cgcgaaggca cggcggttcc ggctgtgtgg
    52561 ttcagcattg aactgatttt cgggctgttt gccctgaaaa atggcccgga agcctgggcg
    52621 ggattcgtcg actttttaca aaacccggtt atcgtgatca ttaacctgat cactctggcg
    52681 gcagccctgc tgcacaccaa aacctggttt gagctggcac caaaagcagc caatatcatt
    52741 gtaaaagacg aaaaaatggg accagagcca attatcaaaa gtctctgggc ggtaactgtg
    52801 gttgccacca tcgtaatcct gtttgttgcc ctgtactggt aaggagcctg agatgattaa
    52861 tccaaatcca aagcgttctg acgaaccggt attctggggc ctcttcgggg ccggtggtat
    52921 gtggagcgca attattgcgc cggtgatgat cctgctggtg ggtattctgc tgccactggg
    52981 gctatttccg ggtgatgcgc tgagctacga gcgcgttctg gcgttcgcgc agagcttcat
    53041 tggtcgcgta ttcctgttcc tgatgatcgt tctgccgctg tggtgtggtt tacaccgtat
    53101 gcaccacgcg atgcacgatc tgaaaatcca cgtacctgcg ggtaaatggg ttttctacgg
    53161 tctggctgct atcctgacag ttgtcacact gattggtgtc gttacaatct aacgcatcgc
    53221 caatgtaaat ccggcccgcc tatggcgggc cgttttgtat ggaaaccaga ccttatgttc
    53281 aaaacgacgc tctgcacctt attaattacc gcctcttgct ccacatttgc cgcccctcaa
    53341 caaatcaacg atattgtgca tcgcacaatt accccgctta tagagcaaca aaagatcccc
    53401 ggtatggcgg tggcggtaat ttatcagggt aaaccttatt actttacctg gggctatgcg
    53461 gacatcgcca aaaagcagcc cgtcacacag caaacgttgt ttgagttagg ttcggtcagc
    53521 aaaacattta cgggcgtgct tggtggcgac gctattgctc gaggggaaat caagttaagc
    53581 gatcccacaa caaaatactg gcctgaactt accgctaaac agtggaatgg gatcacacta
    53641 ttacatctcg caacctacac cgctggcggc ctgccattgc aggtgccgga tgaggtgaaa
    53701 tcctcaagcg acttgctgcg cttctatcaa aactggcagc ctgcatgggc tccaggaaca
    53761 caacgtctgt atgccaactc cagtatcggt ttgttcggcg cactggctgt gaagccgtct
    53821 ggtttgagtt ttgagcaggc gatgaaaact cgtgtcttcc agccactcaa actcaatcat
    53881 acgtggatta atgtaccgtc cgcagaagaa aagaactacg cctggggata tcgcgaaggt
    53941 aaggcagtac atgtttcgcc aggggcgtta gatgctgaag cttatggtgt gaagtcgacc
    54001 attgaagata tggcccgctg ggtgcaaagc aatttaaaac cctttgatat caatgagaaa
    54061 acacttcaac aagggataca actggcacaa tctcgctact ggcaaaccgg cgatatgtat
    54121 cagggtctgg gctgggaaat gctggactgg ccggtaaatc ctgacatcat cattaacggc
    54181 agtgacaata aaattgcact ggcagcacgc cccgtaaaac cgattacgcc cccaactcct
    54241 gcagtacgtg catcatgggt acataaaacg ggggcgaccg gcggatttgg tagctatgtc
    54301 gcgtttattc cagaaaaaga gctgggtatc gtgatgctgg ctaacaaaaa ctatcccaat
    54361 ccagcgagag ttaccgccgc ctggcagatt ctcaacactc tacagtaaaa tttcatcggg
    54421 tccgaatttt cggacctttt ctccgctttt ccttgctgtc atctacactt agaaaaaacc
    54481 agtaaggaaa catttatgcg cctgctccct ctcgttgccg cagcgacagc tgcatttctg
    54541 gtcgttgcct gcagttctcc tacgccgccg cgtggcgtga ccgtagtgaa taatttcgac
    54601 gctaaacgct atctcggcac ctggtatgag attgcccgtt ttgatcaccg ctttgaacgt
    54661 ggactggaaa aagtcaccgc aacatacagc ctgcgggatg acggcggcct gaatgtcatt
    54721 aataaaggct ataaccctga cagaggaatg tggcagcaga gtgaagggaa agcgtacttt
    54781 accggcgcac caactcgcgc tgcgctgaaa gtgtcattct ttggtccttt ctatggcggt
    54841 tataacgtta ttgcactcga tcgggaatac cgccatgcgc tggtttgcgg cccggaccgc
    54901 gactacctgt ggatactctc tcgcacgcca accatttctg acgaagtgaa acaggagatg
    54961 ctggcagtcg cgacccggga agggtttgat gtcagtaagt ttatttgggt acagcagccc
    55021 ggtagttagt gagtgctgag tttcagacca ataatcccca atacgattaa cgccagactc
    55081 gccaggcgca tcgggttagc ggactcaccg agcagcacaa tgccggtgat ggccgcgccg
    55141 actgcgccaa tacccgtcca cacggcataa gccgtcccta ctggtaacga tttcatcgcc
    55201 caggcaagta gcgccatact gacaatcatc gccatcacgg taataacact tggcgtcaaa
    55261 cgactaaagc cgtgggtata tttcaggcca acggcccata ccacttccag cagaccagca
    55321 ataactaaga taatccagga catatcaggc tccggagaat tggggccgtc cccggtgaaa
    55381 agaggcgttt gcaggtcgtc ctgcaaagct gatggttgaa cggatatttt gccaggtggc
    55441 aggggaattt caactattac ctttgtgact attgcaaaaa aacaaacggc acgacataac
    55501 tgtcgtaccg ttattgctta ctgctgcgct ttcgttgctg cgccagaaat cgcgttaccg
    55561 ccatcagaaa tgtcttcacc aacgccacgc gtggtgttgc aggcagttaa tactgttgaa
    55621 agcaccagaa cagaaaagat cgctgcaatt gtcttcttca ccataacgtc ttccttttag
    55681 gtaatgttat ttatgtttgc ctatagcaaa ttaataatag acaacatcac caaaaatgat
    55741 ggaaatctga tgatttttcg gaagagaaga aaaattagct ggcagcgcgg gagatggaat
    55801 taccgagatg tttaatgtct tcaccaaaac cgcgagcggt gttacagccc gtgagcagcg
    55861 cactggcaag caaaacaaga acgataaggc gtttcatcat ctctgacctg aataagtgat
    55921 ggtgcagcct gcaggccgca ccacaaccgc attacttcac gcgagagacg tattcaccag
    55981 agcgggtatc cactttgatg acttcgccga tctgcacgaa cagcggaact ttaaccacag
    56041 cgccagtaga cagggtagct ggtttgccgc cagtacctgc ggtatcacct ttcaggcccg
    56101 gatcggtatc aacgatttcc agttcaacga agttcggcgg agtaacggag atcggctgac
    56161 cattccacag agttacgata cactctgcct gatcaagcag ccatttcgcg ttgtcgccaa
    56221 ttgcttttgc gtcagcagac agctgctcga aagtttcgtt gttcatgaag tgccagaact
    56281 caccgtcgtt gtacaggtaa gtcaggttca tatcgacaac atcagcgcct tcagcggaat
    56341 cagtagattt gaaggttttt tctacgcgag taccggtcag cagacgacgc agtttaacgc
    56401 gagcaaatgc ctggccttta cccggtttta cgaattcact cgcttcaacc gcgtaaggtt
    56461 cgccgtctaa catgatttta agaccagcac gaaaatcgtt gctatagtac gttgccataa
    56521 ggccctctga aatttgttaa ttggtagcta agccacaaaa tggcgcatat tgtaacccta
    56581 aataccccat ccagagaaga ttggttaacg caacttgccg atgttgtgac cgatcctgat
    56641 gaacttctgc gtcttttgaa tatagacgcg gacgaaaaac tgttggccgg acgcagcgcc
    56701 aaaaagctgt ttgccctgcg tgtgccccgc tcatttatcg atcgcatgga gaaaggcaat
    56761 ccgaacgatc cgcttttgcg tcaggtaatt acctcgcaag atgagtttgt cgtcgcgccc
    56821 ggattctcca ccgacccgct ggaagaacag cacagcgtag tgccaggttt gttgcataaa
    56881 taccacaacc gggcgctttt gctggtcaaa ggcggctgcg cggtaaattg ccgctattgc
    56941 ttccgtcgcc acttccccta tgccgaaaat cagggcaaca agcgtaactg gcagattgca
    57001 cttgagtatg ttgctgcgca tccggaactg gacgagatga ttttctccgg cggcgatccg
    57061 ctgatggcga aagatcacga gctggactgg ttgctcacac aactggaagc catcccgcat
    57121 atcaaacgtc tgcggattca cagccgtctg ccgattgtga tcccggcacg catcacagat
    57181 gcgctggttg aacgcttttc acattctacg ctgcaaatct tgctggtgaa tcacatcaac
    57241 catgccaatg agatagatga aacattccgt caggcgatgg ctaagttgcg ccgggtaggc
    57301 gttactttgc tcaaccagag cgttctgtta cgtgatgtga acgataacgc acaaacgctg
    57361 gcaaacctga gtaatgcgtt gttcgatgcc ggcgtgatgc cgtattacct gcatgtgctc
    57421 gataaagtac agggcgcggc gcattttatg gtgagtgatg acgaagcacg gcagattatg
    57481 cgtgagttgc tgacactggt gtcgggatat ctggtgccta aactggcgcg agaaattggc
    57541 ggcgaaccca gcaaaacgcc gctggatctc cagctacgcc agcagtaact gattaaacag
    57601 ggaagtttaa tctttgcccg ttgcataacc tttaattatt catatacatt gctgttgtta
    57661 ctctttatat aaccaacacc aataattaac ctttcttttc tgtttatata gagtgagcac
    57721 ggctatttat gttattttaa caactattac taccaagccc atttatctgg cttataaact
    57781 aaaattaatt atttaattga gaaatggtta gggagaacct acatggccat aagcatcaag
    57841 ggtgttaata ccggtgttat tcgcaagagt aataatttca ttgcactggc gctgaaaatt
    57901 aaagagcctc gcaataaaga atcgttgttc tttctgtctg tcatggaact gcgcgacctt
    57961 ctgattgcgc tggaaagccg tttgcatcaa aagcataaac tggatgctgc tgcacgcctt
    58021 cagtatgagc aagctcgcga taaagtcatt aagaaaatgg cagaaaatat cccagaaatt
    58081 ctggttgatg agcttaaaaa tgccgatatt aatcgtcggg ttaacacgct ggaacttacc
    58141 gataatcagg gtgaaaatct taccttcgta ttaacgctac atgacggtag cacatgtgag
    58201 ttagtcatca atgaattgca gattgaaatg ctggcgcgag caatcattca tgcaattaat
    58261 aatgcggaaa tgcgtgaact ggcattacgt attacctcac tgttagattt cctgccgtta
    58321 tacgacgttg actgccagga caatggcaat ctggaatatg acacctattc ccaaccggaa
    58381 tggaaacata acttgttcaa ccattatctg gcggtgcttt accgctttaa agataaaagt
    58441 ggcaaagaac aatttagcgg cgcagtggtg aaaacgcgtg aagcaacgcc gggtaaagaa
    58501 gtggaggcga tcactcgccg aatgcttgat ttcagccccc gactgaaaaa actcgcgggt
    58561 gtgccatgtc aggtctatgt tcgcaccgta gcggcaaata atgcccaacc gctaacccag
    58621 gatcaatgct tacgcgcgct gcatcactta cgtgttcaat ccaccagcaa aactgcaccg
    58681 caggcgaaat aatcccatct gttttactac gaggctggaa gtatccagcc tctggatccc
    58741 atcagttcgg gcacttatat acctggccga taatctggct atccgtcggg acaaaactgg
    58801 acaacattcc ctgcgacggg ctgctgatgc cataaatcac gttaccgccc attgcagccg
    58861 cctggttgcg cagatcgttt gctgcgccgc gcatagaacc gccttcttca ccgtgttgcc
    58921 cggaaagcca gttgctttgc ttacctgtcg cagtaccaat cagctggcac tctgcgcctg
    58981 gctgctcgtc cacaatgcgt acactctgac cggcagcact caattcgttg ctggagccac
    59041 aacccgccat cagtagcgcg gcaccgacaa tccctgctaa gtattttacg tgcatgttat
    59101 tccccataat caatgagctg gacgccactc gcgtctgtgt gtgaatctta tactaaaaag
    59161 atgactaaaa gaaaaacccc cagacatttc tgcccggggg tttgtttatt tctgcgaggt
    59221 gcagggcaat tacatcatgc cgcccatgcc acccatgccg cccataccgc cagcagcgcc
    59281 taagtcagct gcatcgtttt tcggcaggtc ggtaaccatg cactcggtgg tgatcatcag
    59341 gccagccaca gaagccgcgt actgcagagc agaacgggtt actttagttg gatccaggat
    59401 acccatgtcg atcatgttgc cgtattcttc ggttgctgcg ttgtaaccgt agttgccgtc
    59461 gccgccttta acggtgttag caacaacaga cggttcttcg ccgcagttca ggacgatctg
    59521 acgcagcgga gcttccattg cacgcagtgc aactttgata cccacgttct ggtcttcgtt
    59581 ctgaccacgc aggtcagcca gtttagacgc tacgcggatc agcgcaacac caccaccagc
    59641 aaccacgcct tcttctaccg cagcacgggt cgcgtgcagg gcgtcttcaa cgcgtgcttt
    59701 tttctctttc atttcaactt cggtagcagc acctactttg ataactgcaa cgccgcctgc
    59761 cagtttcgct acgcgctcct gcagtttttc acggtcgtag tcagaagttg cttcttcaat
    59821 ctgctgacgg atctgagcaa cacggccctg gattgcagct tcttcaccca cgccatcgat
    59881 gatggtggta gtgtctttgt tgatcacaac acgtttagcc tgacccaggt cttccagggt
    59941 tgctttttcc agctccatac cgatctcttc agagattacg gtaccgccag tcagggttgc
    60001 gatatcctgc agcatagctt tacgacgatc gccgaagccc ggagctttaa ccgcagcaac
    60061 tttcacgatg ccacgcatgg tgttaacaac cagagttgcc agcgcttcgc cttcaacatc
    60121 ttcagcgatg atcagcagcg gtttgcctgc tttggcaaca gcttccagaa ccggcagcat
    60181 ttcgcggatg ttggagattt tcttgtcagc cagcaggatg aacgggcttt ccagttctac
    60241 tgcgccagtt tccggcttgt tgatgaagta aggagacagg tagccacggt cgaactgcat
    60301 accttcaacc acgtccagtt cgtcctgcag accggtaccg tcttcaacgg tgataacacc
    60361 ttctttaccg actttgtcca tcgcttcagc gatcagttta cctacggttt cgtcggagtt
    60421 agcggagatg gtaccaacct gagcaatcgc tttagagtca gagcacggta cggacagcgc
    60481 tttcagttct tcaactgcag cggtaacagc tttgtcgata ccacgtttca ggtccatcgg
    60541 gttcatgccc gcagcaacag ctttcagacc ttcagtgatg atagcctgag ccagtacggt
    60601 tgcagtggtg gtaccgtcgc ctgcagcgtc gttcgcttta gaggcaactt ctttcaccat
    60661 ctgcgcaccc atattttcga acttgtcttc cagttcgatt tcacgagcaa cggaaacacc
    60721 atctttggtg atggtcggtg caccgaaaga tttatccaga actacgttac ggcctttcgg
    60781 accgagggta actttcactg catctgccag tacgtttacg ccgcgcagca ttttcacacg
    60841 agcgtcgtta ccgaatttta cgtctttagc tgccattatc tttattcctt aaattcgtat
    60901 gttcagtgtc gtgcgcggat tacgcttcaa caattgccag aatgtcgctt tcggacatga
    60961 tcaacacttc ttcattgtcg atcttctcag atttcacacc gtagccatcg ttgaaaataa
    61021 cgatgtcgcc aactttcaca tccagcggct tcacttcgcc attttcaagg atacggccat
    61081 tgccgacagc cagcacttcg ccgcgggtgg atttagccgc tgcagagccg gtcagaacga
    61141 tgccgccagc agatttagtt tcaacttctt tacgcttgac gatcacgcga tcatgcaatg
    61201 gacgaatatt cattgataac tctcctttga gaaagtccgt atctgttatg ggtgacgccg
    61261 gagcttacgt ggtttcccgg ctggtgacca gagaaatggg gatgaggctt cgccccttca
    61321 agggggaaaa agaaaaaaaa ttctgatcag cacaaaatcg ggtgaaaacc ctgattcacc
    61381 tcacatttca tcgcaatttc ttcatcaccg tcataagcga atctgattgt gctaccatcg
    61441 aaaatctacg cagttgccca taatttgggc gcaatcggac atcaacccat gagtggactc
    61501 aaacaagaac tggggctggc ccagggcatt ggcctgctat cgacgtcatt attaggcact
    61561 ggcgtgtttg ccgttcctgc gttagctgcg ctggtagcgg gcaataacag cctgtgggcg
    61621 tggcccgttt tgattatctt agtgttcccg attgcgattg tgtttgcgat tctgggtcgc
    61681 cactatccca gcgcaggcgg tgtcgcgcac ttcgtcggta tggcgtttgg ttcgcggctt
    61741 gagcgagtca ccggctggtt gtttttatcg gtcattcccg tgggtttgcc tgccgcgcta
    61801 caaattgccg ccgggttcgg ccaggcaatg tttggctggc atagctggca actgttgttg
    61861 gcagaactcg gtacgctggc gctggtgtgg tatatcggta ctcgcggtgc cagttccagt
    61921 gctaatctac aaacagttat tgccggactt atcgtcgcgc tgattgtcgc tatctggtgg
    61981 gcgggcgata tcaaacctgc gaatatcccc ttccctgcac ctggtaatat cgaacttacc
    62041 gggttatttg ctgcgttatc agtgatgttc tggtgctttg tcggtctgga ggcatttgcc
    62101 catcttgcct cggaatttaa aaatccagag cgtgattttc ctcgtgcttt gatgattggt
    62161 ctgctgctgg caggattagt ctactggggc tgtacggtag tcgtcttaca cttcgacgcc
    62221 tatggtgaac aaatggccgc ggcagcatcg cttccaaaaa ttgtagtgca actgttcggt
    62281 gtaggagcgt tatggattgc ctgcgtgatt ggctatctgg cctgctttgc cagtctcaac
    62341 atttatatac agagcttcgc ccgcctggtc tggtcgcagg cgcaacataa tcctgaccac
    62401 tacctggcac gcctctcttc tcgtcatatc ccgaataatg ccctcaatgc ggtgctcggc
    62461 tgctgcgtgg tgagcacgtt ggtgattcat gctttagaga tcaatctgga cgctcttatt
    62521 atttatgcca atggcatctt tattatgatt tatctgttat gcatgctggc aggttgtaaa
    62581 ttattgcaag gacgttatcg actactggcg gtggttggcg ggctgttatg cgttctgtta
    62641 ctggcaatgg tcggctggaa aagtctctat gcgctgatca tgctggcggg gttatggctg
    62701 tttctgccaa aacgaaaaac gccggaaaat ggcataacca cataatccgg cgtttcgaca
    62761 ttaatcctgg cgatcgtctt tatgatcaag gcggtcgcgg tcatcatcct ttctctggta
    62821 ctcaccatca aaagtattac cgccaccggt cccggcgcta aaaccgccgc ctggcatgcg
    62881 agaaaagcgc aaatgtggca tcaacttcac tgtcagatgc ttttgcaccg gcggcaataa
    62941 aagtagaaga ccgaggaagt cggtaaaaaa gcccggtaat aaaagcagca aaccggcaat
    63001 gatcagcgaa acacttttaa tcatctccgc cgctgggttt tcacccgccg ccattttttg
    63061 ctgcatcagc acgaaattct taaagccctg gttgcgtacc agtgacatac cgataactga
    63121 cgtgaatata accagcacga gggtcagcaa tacccccaat acatgggcaa cctgaataaa
    63181 gattgaaatc tcaatataga cataaaggaa aatggcaata aaaggtaacc agcgcaaagg
    63241 tttctcctgt aatagcagcc ggttaacccc ggctacctga atgggttgcg aatcgcgttt
    63301 agcttatatt gtggtcatta gcaaaatttc aagatgtttg cgcaactatt tttggtagta
    63361 atcccaaagc ggtgatctat ttcacaaatt aataattaag gggtaaaaac cgacacttaa
    63421 agtgatccag attacggtag aaatcctcaa gcagcatatg atctcgggta ttcggtcgat
    63481 gcaggggata atcgtcggtc gaaaaacatt cgaaaccaca tatattctgt gtgtttaaag
    63541 caaatcattg gcagcttgaa aaagaaggtt cacatgtcaa acaacattcg tatcgaagaa
    63601 gatctgttgg gtaccaggga agttccagct gatgcctact atggtgttca cactctgaga
    63661 gcgattgaaa acttctatat cagcaacaac aaaatcagtg atattcctga atttgttcgc
    63721 ggtatggtaa tggttaaaaa agccgcagct atggcaaaca aagagctgca aaccattcct
    63781 aaaagtgtag cgaatgccat cattgccgca tgtgatgaag tcctgaacaa cggaaaatgc
    63841 atggatcagt tcccggtaga cgtctaccag ggcggcgcag gtacttccgt aaacatgaac
    63901 accaacgaag tgctggccaa tatcggtctg gaactgatgg gtcaccagaa aggtgaatat
    63961 cagtacctga acccgaacga ccatgttaac aaatgtcagt ccactaacga cgcctacccg
    64021 accggtttcc gtatcgcagt ttactcttcc ctgattaagc tggtagatgc gattaaccaa
    64081 ctgcgtgaag gctttgaacg taaagctgtc gaattccagg acatcctgaa aatgggtcgt
    64141 acacagctgc aggacgcagt accgatgacc ctcggccagg aattccgcgc tttcagcatc
    64201 ctgctgaaag aagaagttaa aaacatccaa cgtaccgctg aactgctgct ggaagttaac
    64261 cttggcgcaa cagcaatcgg tactggtctg aacacgccga aagagtactc tccgctggca
    64321 gtgaaaaaac tggctgaagt cactggcttc ccatgcgtac cggctgaaga cctgatcgaa
    64381 gcgacctctg actgcggcgc ttatgttatg gttcacggcg cgctgaaacg cctggctgtg
    64441 aagatgtcca aaatctgtaa cgacctgcgc ttgctctctt ctggcccacg tgccggcctg
    64501 aacgagatca acctgccgga actgcaggcg ggctcttcca tcatgccagc taaagtaaac
    64561 ccggttgttc cggaagtggt taaccaggta tgcttcaaag tcatcggtaa cgacaccact
    64621 gttaccatgg cagcagaagc aggtcagctg cagttgaacg ttatggagcc ggtcattggc
    64681 caggccatgt ttgaatccgt tcacattctg accaacgctt gctacaacct gctggaaaaa
    64741 tgcattaacg gcatcactgc taacaaagaa gtgtgcgaag gttacgttta caactctatc
    64801 ggtatcgtta cttacctgaa cccgttcatc ggtcaccaca acggtgacat cgtgggtaaa
    64861 atctgtgccg aaaccggtaa gagtgtacgt gaagtcgttc tggaacgcgg tctgttgact
    64921 gaagcggaac ttgacgatat tttctccgta cagaatctga tgcacccggc ttacaaagca
    64981 aaacgctata ctgatgaaag cgaacagtaa tcgtacaggg tagtacaaat aaagaaggca
    65041 cgtcagatga cgtgcctttt ttcttgtgag cagtaactta aaaataacaa tctaatatca
    65101 acttgttaaa aaacaaggaa ggctaatatg ctagttgtag aactcatcat agttttgctg
    65161 gcgatcttct tgggcgccag attgggggga ataggtattg gttttgcagg cggattgggg
    65221 gtgctggttc ttgccgctat tggcgttaaa cccggtaaca tcccgttcga tgtcatttcc
    65281 attatcatgg cggttatcgc cgctatttct gccatgcagg ttgctggcgg tctggactat
    65341 ctggttcatc agacagaaaa gctgctgcgc cgtaacccga aatacatcac gatcctcgca
    65401 ccgatcgtga cctatttcct gactatcttt gctggtactg gcaacatctc tctggcgaca
    65461 ctgccagtta tcgctgaagt tgcgaaggaa caaggcgtta aaccttgccg tccgctgtct
    65521 actgcagtgg tatccgcgca gattgcgatc accgcatcgc caatctcagc agcagtggtt
    65581 tacatgtctt ccgtgatgga aggtcatggc atcagctacc tccatctgct ctccgtggtc
    65641 atcccgtcca ccctgctggc ggttctggtg atgtccttcc tggtcactat gctgttcaac
    65701 tccaaactct ctgacgatcc gatttatcgc aagcgtctgg aagagggcct ggttgaactg
    65761 cgcggtgaaa agcagattga aatcaaatcc ggtgcaaaaa cgtccgtctg gctgttcctg
    65821 ctgggcgtag ttggcgtggt tatctatgca atcatcaaca gcccaagcat gggtctggtt
    65881 gaaaaaccgc tgatgaacac caccaacgca atcctgatca tcatgctcag cgttgcaact
    65941 ctgaccaccg ttatctgtaa agtcgatacc gacaacatcc tcaactccag caccttcaaa
    66001 gcaggtatga gcgcctgtat ttgtatcctg ggtgttgcgt ggctgggcga tactttcgtt
    66061 tccaacaaca tcgactggat caaagatacc gctggtgaag tgattcaggg tcatccgtgg
    66121 ctgctggccg tcatcttctt ctttgcttct gctctgctgt actctcaggc tgcaaccgca
    66181 aaagcactga tgccgatggc tctggcactg aacgtttctc cgctgaccgc tgttgcttct
    66241 ttcgctgcgg tgtctggtct gttcattctg ccgacctacc cgacgctggt tgctgcggta
    66301 cagatggatg acacgggtac tacccgtatc ggtaaattcg tcttcaacca tccgttcttc
    66361 atcccgggta ctctgggtgt tgccctggcc gtttgcttcg gcttcgtgct gggtagcttc
    66421 atgctgtaat gatcaatcgc ggggcgttca cgccccgctt tctttcccgc cgactaacat
    66481 ccttcccccg tccgttgtat agtgacctct ctcttgcggt tccatctgtt cttgcgaggt
    66541 gtttatgctt gatgaaaaaa gttcgaatac cgcgtctgtc gtggtgctat gtaccgcacc
    66601 ggatgaagcg acagcccagg atttagccgc caaagtgctg gcggaaaaac tggcggcctg
    66661 cgcgaccttg atccccggcg ctacctctct ctattactgg gaaggtaagc tggagcaaga
    66721 atacgaagtg cagatgattt taaaaactac cgtatctcac cagcaggcac tgctggaatg
    66781 cctgaagtct catcatccat atcaaacccc ggaacttctg gttttacctg ttacacacgg
    66841 agacacagat tacctctcat ggctcaacgc atctttacgc tgatcctgct actttgcagc
    66901 acttccgttt ttgccggatt attcgacgcg ccgggacgtt cacaatttgt ccccgcggat
    66961 caagcctttg cttttgattt tcagcaaaag caacatgacc ttaatctgac ctggcagatc
    67021 aaagacggtt actacctcta ccgtaaacag atccgcatta cgccggaaca cgcgaaaatt
    67081 gccgacgtgc agctgccaca aggcgtctgg catgaagatg agttttacgg caaaagcgag
    67141 atttaccgcg atcggctgac gcttcccgta accatcaacc aggcgagtgc gggagcgacg
    67201 ttaactgtca cctaccaggg ctgtgctgat gccggtttct gttatccgcc agaaaccaaa
    67261 accgttccgt taagcgaagt ggtcgccaac aacgcagcgt cacagcctgt gtctgttccg
    67321 cagcaagagc agcacaccgc gcaattgccc ttttccgcgc tctgggcgtt gttgatcggt
    67381 attggtatcg cctttacgcc atgcgtgctg ccaatgtacc ctctgatttc tggtatcgtg
    67441 ctgggcggta aacagcggct ctccactgcc agagcattgt tgctgacctt tatttatgtg
    67501 caggggatgg cactgactta cacggcgctg ggtctggtag ttgccgccgc agggttacag
    67561 ttccaggcgg cgctacagca cccatacgtg ctcattggcc tcgctatcgt ctttaccttg
    67621 ctggcgatgt caatgtttgg cttgtttacc ctgcaactcc cctcttcgct gcaaacgcgc
    67681 ctcacgctga tgagcaatcg ccaacagggc ggctcacctg gcggtgtgtt tgttatggga
    67741 gcgattgccg gactgatctg ttcaccatgc accaccgcac cgcttagcgc gattctgttg
    67801 tatatcgccc aaagcgggaa catgtggctg gggggcggca cgctttatct ctatgcgttg
    67861 ggcatgggcc tgccgctgat gctaataacc gtctttggta accgcctgct gccgaaaagc
    67921 ggcccgtgga tggaacaagt caaaaccgcg tttggttttg tgatcctcgc actgccggtc
    67981 ttcctgctgg agcgagtgat tggtgatata tggggattac gcttgtggtc ggcgcttggt
    68041 gtcgcattct ttggctgggc ctttatcacc agcctacagg ccaaacgcgg ctggatgcgc
    68101 gtggtgcaaa taatcctgct ggcagcggca ttggttagcg tgcgcccact tcaggattgg
    68161 gcatttggtg cgacgcatac cgtgcaaact cagacgcatc tcaactttac acaaatcaaa
    68221 acggtagatg agttaaatca ggcgctcgtt gaagccaaag gcaaaccagt gatgttagat
    68281 ctctatgccg actggtgcgt cgcctgtaaa gagtttgaga aatacacctt cagcgacccg
    68341 caggtgcaaa aagcgttagc agacacggtc ttacttcagg ctaacgtcac tgccaacgac
    68401 gcccaagatg tggcgctgtt aaagcatctt aatgtccttg gcctaccgac aattctcttt
    68461 tttgacggac aaggccagga gcatccacaa gaacgcgtca cgggctttat ggatgctgaa
    68521 accttcagcg cacatttgcg cgatcgccaa ccgtgaacga cacttgcagt gggaaagacg
    68581 gaggaaaata gcgtgcaacg tgaagatgta ctgggagaag ccctgaaatt attagaatta
    68641 caagggattg ccaacaccac gctggagatg gttgctgaac gtgtggatta tccactggac
    68701 gagctgcgcc gcttctggcc agacaaagag gcaatcctct acgatgcgct gcgttatctt
    68761 agccaacaga tagatgcctg gcgtcgtcag ctgatgctgg acgaaacgca aaccgccgaa
    68821 caaaagttgc tggcacgtta tcaggcgtta tcggagtgcg ttaaaaacaa ccgctatccg
    68881 ggctgtctgt ttatcgctgc ctgtacgttt tatcccgatc ctggccaccc tattcatcaa
    68941 ctagccgatc agcaaaaaag cgcggcctac gatttcaccc acgaactgtt aaccacgctg
    69001 gaagttgacg atccggcgat ggtagcaaag cagatggaac tggtgctgga aggctgttta
    69061 agccgaatgc tggtgaatcg tagccaggcg gatgtcgaca ccgcacatcg gctggcggaa
    69121 gatatcctgc gcttcgcccg ctgccgccag ggtggcgcac tgacctgaca gaaacacaga
    69181 aaagaagcga tttgccgcaa tcttaagcag ttgaatcgct tttactgaaa ttaggttgac
    69241 gagatgtgca gattacggtt taatgcgccc cgttgcccgg atagctcagt cggtagagca
    69301 ggggattgaa aatccccgtg tccttggttc gattccgagt ccgggcacca ctttcacttc
    69361 cagattcatc tcccgaactc cacgaaactc aagtataatc acatcgttac aagcaatctg
    69421 aacattctga actccaccag actctactga actctctctc aatcaagcgt tcattggggg
    69481 ccccggtggg ggctcattgt gttcagtcag tttcaggagc ccccatatgg cacttacagc
    69541 cagacaggtc gaaacagcca gacctaaaga aaaagactat aaactctctg acgaacgtgg
    69601 tttatatctg ctggtaaaaa ccacgggtgc ccgctactgg cggcttaaat accggatagc
    69661 aggaaaagag aaaaaactgg ccctcggcgt ctatcccgac gtctcccttg ctgaggccag
    69721 aatcaaacgc gacgatgccc gaaaaatcat ctccgaaggt ggtgacccgg gcgaaaagaa
    69781 gcgaaaggaa aaactcactc agaaaatctc tgccaccaat acgttccatg ccctcgctac
    69841 ggaatggcac cagcataaat ctttgtcatg gtctgaaagt tacgccagaa gcgtactgga
    69901 agcgctggat aaagatattt tcccgtatct gggcaaacga agcgttacgg atatcctccc
    69961 gctggaaatg ctggaaattc tgcgccgcat agaaaaacgt ggctcgctgg aaaaacttcg
    70021 taaggtgcgt caatactgta atcagatttt tcgttatgcc atcgccaccg gacgagccac
    70081 tgtcaatccg gcatctgaac tgaccagtac gctggcggcg ccaaaagctg cacatttccc
    70141 ccacctgaga gcagatgagc tccctgtttt tctccggaag ctcgctgagt atcatggcag
    70201 tcctgttacc cgcatggcga caaatctgct gcttctgaca ggcctcagaa cgattgaact
    70261 acggtccgct gaatggtcag aaattgattt tgataatgcc ctgtggacaa tccctgaaag
    70321 ccgcatgaaa atgcgacgta aacatgtcgt accactgtca cgacaggcca ctgacattct
    70381 gctgcagctc aaaactttct ccggacaata ccggctggtt ttcccgggac gttgtgatat
    70441 caacaagcca atgagcgaag ccagcatcaa tatggtgctc aaacgtatcg gttacgatgg
    70501 cagggcaacc ggtcatggtt ttcgtcacac catgagtacc attctgcacg aacagggctt
    70561 taattctgcc tggattgaaa tgcagttagc tcatgtggat aaaaacgcca tcaggggtac
    70621 ctataatcat gcccagtatc tcgatggtcg ccgtgaaatg atgcaatggt acgcagatta
    70681 cattgattcg ctttccaggc aagagagtca gggttaagaa cacttgttct gatattttct
    70741 ctcaatccac aagttctcct ccggcctcca ggtccggagg aattttgttt ttatgcttta
    70801 tttttagata ttttttgttt tttgacaaaa tatttaatat tgcatctgta aacaatctca
    70861 aaacatcaca ccgttatgga ccgctaacaa ttttgatacc tggagtttca atcatcatcc
    70921 tgtcgttatt tcagcgccat cacaaaaaaa aatcgattaa tatgctgttt cagctgacaa
    70981 ctcacaatac accggaatgc agataaagat aaattttcat tctcatgtaa aatcactgtc
    71041 gatgatattg taaacaacat tcatcccgga caaaaacagg acttgataca caacatcacg
    71101 acttttatgc ttttcagatt atgtccccat ccgttttaca ggctcagaaa atgaacgaca
    71161 gattacgctt tgaagtgaac gataaccagg ggcgtttcgt atttccggat acctggtttg
    71221 gcccattact gggggagttt gaagaagtgc ttgatgcata cgacactgat gaaatcagcg
    71281 agacgagtta catcaacaaa ctcagacacc ttgctcaacg ggagcctgac tttattgata
    71341 tacacgctca tctggcatat gcatttctgg agcagaatgc cccgcgtaag gctctgaacg
    71401 ccgcgcttaa aggactggcg gccggtaatc gtctcatccc ggaaagtttc agtggtgaaa
    71461 tcatctggat gcatcccgaa aaccggcctt atctgcgcgc gctttatgca gcaattctcg
    71521 caaatgtgca cttacagcgt catcaggatg cggttatgct tactgataaa atactggcat
    71581 acaatcctga agataatcag ggtgcccgct ggttgttggg ttcagaactg ttacgaacag
    71641 gtgatcatga gcgagccttc agtgtactga aaaaacatgc tgatgaattt tcaccttact
    71701 ggtatgaact tggtcttctg cattttctta atggggaaca ggtaaaagct gcaactgcat
    71761 ttcgtcgcgg atttgcggca aatacttaca tcgcggaaat gctgtgcgga aatctgcatc
    71821 catttcctct tgctgtatgg cataactatt ctgccgggcc ggatactgcc gaagattatt
    71881 atgcaacata ctcaccatta tggggacaat atccggaagc attgctgttt gtaaactggc
    71941 tctataacca ttcctctgtg ctgcatgaac gggcagaaat cattaaatgc gccgaaatgc
    72001 tgatgcagga agatgatttc gaaatatgcg aaagtatttt aagacagcag gagaagttgc
    72061 gtgaaagaat tgatgagacg ctttctgaga aaattgtaca gaaatgcaga aatatgaatg
    72121 gtgaatatgt ctggccctgg atattgccgt tttcagcagc aggcaggaaa cattctggca
    72181 tacagtatca gtagatatcg cattagtgta tcctgcacac aagtaataat ttatccacca
    72241 ataataacac tgttaatgtc ccctttccct ggttgtcagc caggggttat cttctgaata
    72301 tttcttttga aaaggacaac acagtaaatt atttttatga attatcccat ggactcatta
    72361 acaccctttc ataatgcttt attgtcaaac acgttatggc tgacatcaaa aaaaaccgga
    72421 tttcctctgc cagcgggtaa tcacctcccc ggtgttttcg gttggtctgg ttactcctgt
    72481 ctggttatta gcaagataat tgctataaac agtggaaaac tcatcgtaca taacctggtg
    72541 atgaacatta ctcttatttt cccttgcccg gaagaatcag aggttgtgac tcaagactgt
    72601 ctgtcggtac attcttgtct ccgttaaaaa ccataatgag ttcattatct tcgtctgtca
    72661 gcggattgaa tggcggtata ttttcagtac gaatgccggt cagccactga aaaatacctg
    72721 cgaaatgacg ggcactgatt tttctgctga cggactgatg agacgtgatg tcactggcgg
    72781 taataatcag gggaacgctg tagcctccct gcacatgacc atcatgatga acaggattag
    72841 cactgtcgct gaccgacagc ccatggtcag aaaagtaaag catgacgaaa tgacgggaat
    72901 gccggcgaag gataccatca agctgaccga gaaagttatc cagtttactg atgctggcga
    72961 ggtaacaggc aatctttcgg ggatactgct ccaggtaata attcggccag gagttaagtc
    73021 ggtcacacgg attcggatga gagccaatca tgtgcaggaa aatcaccttc ggagaggatt
    73081 tatccgccag cgcacgttct gtttcctgta acaacaacat gtcatccgtt ttacgggaag
    73141 cgaagctgcc tttcttgagg aaaacggtat gctccgcatc agaagcaata acagagatgc
    73201 gtgtgtcatg ctcccccagt tttccttgat tggatatcca ccatgtgctg tatcctgctt
    73261 ttgctgccag cgccaccaca ttgttgccgg aatcagggtt ctgctcatag tcataaatca
    73321 gtgtccggct cagggaagac actgtactgg aggctgtcaa ggtataatcg tcaataaata
    73381 aaccgggtgc cgtattcagc cacggtgtgg ttggtacgag atagccatac actgacatat
    73441 aatccctgcg cacattctca ccagtgacaa taacaatcgt atcatataac ggtgctcccg
    73501 gctggatttt ccagttgtca gccccgtgct gactcagttg ttcataacgc tgcatttcac
    73561 gtaatgtgtc agttgtcccc acaactgttc ctttaaccat ccgcaatggc caacggttta
    73621 cagagcataa tacgaatagc cgtagagcca gccagttacg gtggtcgcga tggtgtgtcc
    73681 gccagaaaat caccacgaat accagaatcg cggcactgac cagaaaatga taaacaggaa
    73741 acacggggcg gttcactttg ctttctcctg tagtgttttc tgtaggttct gaaccggttc
    73801 gagcggggtt gtgaggctga tggcaatccc cgggctcttc ctgcatgtta cactggtact
    73861 gcaagagggg gccttcgctc ctctgacatt actcagtatt ttcactacta caccccatct
    73921 gccatccata acaaccgatt caccccctcg cgaggcgttg ttgcccgtca cccggacaga
    73981 catcgttatg gacttctctt gttgcacgat cgatcaattg ttatgcatgc tgacaccaca
    74041 accccggtgg gtatgaccga tgcctttctc actctcttcc cggtcatcgt cggccttccc
    74101 cgttattgtg gcgtgtcagc accctcgacg gcatttcgat ggctgctcag tgttcactca
    74161 cattacggcc cgcataaccc gttgacttcc tgaagaagtc gtttcaggga gagattcagc
    74221 ccttttgtta cctcctggac cgcttcttgt ccttccagtc ggagcgagag ttagccgggg
    74281 cggactttca ccgacggaaa gatcgtgcat ttcaaggcac acacaacaat atctgcgagc
    74341 gtgctatccg tctggtcgtt atgggacgaa aggcctggtt gttcgctggt tcactgatgg
    74401 ccgggaaccg cgcggcacag ataatgagcc ttctgggaac cgcagtctgg agccacatgc
    74461 ttggctgacg gacgtcctga cgcgtctgcc ggagtggccg gaggagaggt tggctgagtt
    74521 actgcctctt gagggcttta ccttcttcgg gtaagtgata cctgccgtca ggtgtttgtg
    74581 caccgggcca taacctgcag ttgggaattg aacgcctgac aacaggaaat gagctagagc
    74641 agtaaacacg cgggctgaca atgggtcaga atatgagtca gcccggcgtt attcctgggg
    74701 tcagtacttc agatccagcc tccggtacca gactagctag cccctacgca accaaataaa
    74761 tcaaccatcg tcagagtgca atcgttcata tacactgtcg gtgatgcgct gaaaaatgcg
    74821 ttaatgttga gttcctcgaa cccttaccaa ttaagggaca tagccagata cgcaactggc
    74881 tgatgcaagc agtctaatgt ctttagatcc gctggcctgc gggcatgaca atgtcaaaca
    74941 gtcaaagatt gtggcgttga cggtcagtct tggggacaaa aaagctcagt attctcgttg
    75001 gcaaccagat aatttaacag ttgatgctga aacgaaatag gcatcttggt tctggtgttt
    75061 gtaacaaact atcagctaca aaaatatgct caatttgtga catcagtaac aaaacgcgtt
    75121 ttgttatgtg gattgcttgt tttttgatct ggtataacag gtataaaggt atacagaaaa
    75181 gcaaggaaat actgcaaagg aaaacagcta taacgtaagc taaagtaata acctctcagt
    75241 ctttcctcat ttgactaagg gagttttatt caacctgaac ggactacgaa aatgagcaca
    75301 atgaataagt caatttttga agcagggttg aactgtgcca gtggtggaat atctggcgaa
    75361 cattgttttt tgttggtgac ccaaaactgt aggtgggtat cagttttatc tttcatagag
    75421 tgaaatatgt taagaagaaa tggaggaaaa gattgtactg attaggtatt gataaccatc
    75481 aatagtactg gcgtatttga agacaatata attatttctg gatattgttg aggctcccta
    75541 atatttactt taagggctat attagaataa cacaggaaac aaatatggca acagcatggt
    75601 ataaacaagt taatccacca caacggaaag ctcttttttc cgcatggctt ggatatgtat
    75661 ttgatggctt tgattttatg atgatatttt acattcttca tattataaaa gcagatcttg
    75721 gcattacgga tattcaggct actttaatag ggacagtggc cttcatagcc agacctattg
    75781 gaggtggttt ttttggtgcc atggctgata aatatggtcg taagccaatg atgatgtggg
    75841 caattatcat ttactcagtc ggaacaggcc ttagcggtat tgctacaaac ttatatatgc
    75901 tcgcagtttg ccgttttatt gttggcttag ggatgtctgg tgaatatgca tgtgcttcaa
    75961 cttatgcggt agaaagttgg cctaaaaatc ttcaatctaa agctagtgct tttttggtaa
    76021 gtggtttttc tgttggaaat attattgcgg cacaaataat ccctcagttt gctgaagtat
    76081 atggatggag aaactctttt tttataggcc tgttaccagt tttactagtt ctttggatca
    76141 gaaaaagtgc tccagaaagt caggagtgga ttgaagataa atataaggat aaatcaactt
    76201 ttttgtctgt cttcagaaaa ccacatcttt caatctctat gatcgttttc ctcgtctgtt
    76261 tttgtctatt tggtgcaaac tggccgataa acggactact tccttcctac ctggcagata
    76321 atggagttaa tacagtggtt atttcaactc tgatgacaat agcaggttta ggaacactga
    76381 caggtacaat attttttggt tttgttggtg ataagattgg tgtaaaaaaa gcctttgtag
    76441 tcggtctaat aacttcattt gttttccttt gtcctctttt ttttatttct gtgaaaaact
    76501 cttctcttat aggattatgt ctctttggat taatgtttac aaatttaggt attgcagggt
    76561 tggttccaaa atttatatat gattactttc caacaaaatt aagaggatta gggaccggtc
    76621 ttatttataa cttaggggca actggaggaa tggccgcacc tgtattagct acatacattt
    76681 caggatatta tggcttaggt gtttcattat tcattgttac ggttgcattc tctgccttat
    76741 taattttgtt agttggtttt gatattccag gtaaaattta taaactatcc gtggctaaat
    76801 aattggaggc tttatgatta attatggcgt tgttggtgtt ggatactttg gcgctgaatt
    76861 agctcgtttt atgaatatgc atgataatgc aaaaattaca tgtgtatacg atcctgaaaa
    76921 tggagaaaat attgcccgtg aattgcagtg tatcaatatg tcaagcttgg atgctttagt
    76981 ctcaagtaaa ttagtcgatt gcgtgatcgt agccacccca aattatctgc ataaagaacc
    77041 agtaattaaa gcagcaaaga ataagaagca tgttttttgt gaaaaaccaa ttgcattaag
    77101 ttatgaagat tgtgtggata tggtcaaagc gtgtaaagaa gctggtgtga cctttatggc
    77161 cgggcatatt atgaattttt tcaatggggt tcaatatgca cggaagttaa ttaaagaagg
    77221 tgttatcggc gaaatattat catgtcatac taagagaaat ggctgggaaa acaaacaaga
    77281 gagactttcc tggaaaaaga tgaaagaaaa atctggtgga catctatatc atcatataca
    77341 tgagttagat tgtgttcagc atttacttgg agaaatacca gagacggtta ctatgattgg
    77401 tggaaatttg gcccattctg gtccaggatt tggcaatgaa gatgatatgt tatttatgac
    77461 cttggaattc ccgtcaggaa aactagcaac cttagagtgg gggagtgcat ttaactggcc
    77521 ggaacattat gtcatcatca atggaactaa aggctctatt aaaattgata tgcaagaaac
    77581 agcagggtca cttaggattg gcggtcagac aaagcatttt ttggtccatg aaacacaaga
    77641 agaagatgat gatcgtcgga aaggcaatat gacctcagaa atggatggcg ctatagcata
    77701 tggtcatcca ggaaaaaaaa caccattatg gcttgccagt ttaataagaa aggagacgtt
    77761 attcctccat aatatcctct gtggtgcaaa acctgaagaa gattatattg accttctcaa
    77821 tggtgaggcg gccatgtcgg cgattgctac tgctgatgct gccactcttt caagatcgca
    77881 ggacaggaaa gtgaaaatca gtgagatcat taaacataca tcagtaatgt aaaaactata
    77941 cgcctcttga tttaattcag gaggcctttt atgtatgatc aagaatttta tcttcgataa
    78001 tctcataatt ttagcagtgc catttatgat aaaaacatcc cttaaaacca atctaatctt
    78061 tttcttcctc tgtgtatttg ttccccgtat ggcgtcataa tttacgctac gtaatacggg
    78121 atagttacga tacgcagcga tagcgctaag ttttagttaa aatcccccat cagcgggata
    78181 tggggatctc tgggaggttc aggttttagc atcgcgactt taatacgaca gacgctggtg
    78241 acgatgatgc caagtagtga ctcttccgaa taaggttccc actgaccaat tggcgcgcgc
    78301 ttcgcaataa catctccctt cgcccagtat ttgaaccgcc ccggaaatcc tggagactaa
    78361 actccctgag aaagaggtaa acaggatgac taaaaatact cgtttttccc ccgaagtccg
    78421 tcagcgggcg attcgtatgg ttctggaaag tcaggatgaa tatgactcac agtgggcggc
    78481 aatttgttcc attgctccaa agattggctg tacgccggag actctgcgtg tctgggttcg
    78541 ccagcatgag cgggataccg ggggcggtga tggtgggctc accagcgctg aacgtcagcg
    78601 tctgaaagag ctggaacgtg aaaatcgtga actgcgccgc agtaacgata tccttcgcca
    78661 ggcttccgct tattttgcga aggcggagtt cgaccgcctc tggaaaaatg atgccactgc
    78721 tggataagct gcgtgagcag tacggggtcg gaccggtatg cagcgaactg catattgccc
    78781 cgtcaacgta ttaccattgt cagcaacagc gacatcatcc ggataaacgc agtgcccgtg
    78841 cgcagcacga cgactggctg aagagagaga tacagcgcgt atacgatgaa aatcatcagg
    78901 tgtacggtgt gcgtaaagtc tggcgtcagt tgttacggga aggaatcagg gtggccagat
    78961 gtacagtggc acgtctcatg gcggttatgg gacttgccgg tgttctccgg ggtaaaaagg
    79021 tccgtacgac catcagccgg aaagccgttg ccgcaggcga ccgcgtaaac cgtcagttcg
    79081 tggcagaacg acctgaccag ctgtgggtgg ctgattttac ttacgtcagc acatggcagg
    79141 gcttcgtcta tgtggcgttt atcattgatg tgtttgccgg atacatcgtg gggtggcggg
    79201 tctcatcgtc tatggaaacg acattcgttc tggatgcgct ggagcaggcg ttgtgggccc
    79261 gtcgtccgtc tggcaccatc catcacagcg ataaaggctc tcagtatgtg tcactggcct
    79321 atacggagcg actaaaagaa gccggattac tggcatcaac agggagtaca ggcgactcgt
    79381 atgacaacgc gatggctgag agcatcaatg gtctttacaa agcggaggta atacaccgta
    79441 agagctggaa aaaccgtgca gaagtggaac tggccacact aacgtgggtg gactggtata
    79501 acaatcgacg attgctggga aggctgggcc atactcctcc ggcagaagca gaaaaagctt
    79561 attatgcttc catcggaaac gatgatctgg cagcctgagt tcacagataa aacactctcc
    79621 aggaaacccg gggcggttca aacagtcgtt cagtccttta tgttacccgt tatgacaaca
    79681 atcagttggt gatggacgta tgaaccttct gtgcttccgt tgattcttat tgactgataa
    79741 agttttgtct gctgaatctt tatacggaat tctcttcagt atgcctgcac aaattgacta
    79801 tcttgcggat aaatattgtt ttacagaact gaatgaatct ccacgccttt gccagcagtg
    79861 gcaggatgtt ctggaggagt gtcggcagac agaggccgga ccagaagaac ggctgcgtat
    79921 tgccctgctg aatgtggatt acgtcaccag ttttgaactg ccttttcgtc tgcttttaac
    79981 ccgcacgcca cagctgattg ctgcgcttcg agaagaatgg gatatcagcc agaaaaatgt
    80041 ggtgttcaac gataagcgat ttggctgcgt gtacagcctg aaggccagtc tttccggtgt
    80101 gccggataca ttccggtatc atctgtctca ccgtattcgc cggatggttg ggaatgaaaa
    80161 tacatcatca ccctatcagc agattgcccg ggaagtgaaa gtgccccgtg aacggctgaa
    80221 gtatgcgctg gaggcgggat tgctggtgac tgcgctggac ggcctgttct ggtctggcag
    80281 tcagcgtatt gcggctgatg tcctgagact aagacagaac ggaatgccgg tagtgacaac
    80341 gaatgtggag gtgcacgata acctgacagg aacaacccgc aaagtaccgg cataccatct
    80401 ctgatacaac catcaggggg aatattgctc tggcagcatg caaaatggca tctgaatcga
    80461 tcgcagtttg gcattcgaat cgatcatgat ttggcggtgt ttttcaaggt agttgtcgc
//
LOCUS       DAMZFT010000007        74669 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.007, whole genome shotgun sequence.
ACCESSION   DAMZFT010000007 DAMZFT010000000
VERSION     DAMZFT010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 74669)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 74669)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..74669
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.007"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..1141
                     /locus_tag="PN977_000556"
     CDS             <1..1141
                     /locus_tag="PN977_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001093935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HDI6040630.1"
                     /translation="LHSGPPIAWEEMCGPVKGAIIGAMLYEGWATSQQDAENQINAGE
                     IDLAPCHHYHAVGPMAGIISPSMPLWVVENKTNGHRTFSNFNEGLGKVLRFGANNDEV
                     LNRLAWMRDELAPAMKAAIAQHGELELKPLMAQALHMGDEVHNRNAAATGLLIKRLLP
                     ALLACSLPQEHIQRVVAFITGNDHFFLNLSMAACKAMMDAAANVPFSSMVTVMARNGV
                     NFGIRLSGTGDRWFQAPANAVEGLFFPGFGVDDAAADLGDSAITETAGVGGFAMASSP
                     AIVKFVGGTPADATNNSRRMQAVTLGGNPAFTLPALNFAPTAAGIDARKVTDRGILPV
                     INTGIAHKQAGVGQIGAGITTAPMACFVAAVRALAEIVAKENHHG"
     gene            1134..2090
                     /gene="arcC"
                     /locus_tag="PN977_000557"
     CDS             1134..2090
                     /gene="arcC"
                     /locus_tag="PN977_000557"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001606533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HDI6040631.1"
                     /translation="MVKPLAVVAVGGNALIQDEQRNSIPDQYVAVMESVQHIVDMVEA
                     GWDLVLTHGNGPQVGFILRRSELASNEVSPVPLDYAVGDTQGAIGYMFQKALHNELAR
                     RGINKPVIALVTQTRVSPHDDAFASPSKPIGAFLDEATAQQRQQQLGWTLMEDAGRGW
                     RRTVPSPAPLEVIEHDTIAHLVRQGYLVIACGGGGIPVVRDGQQLKGVEAVIDKDLAS
                     ALLASQLGADLLVIPTGVEKVAINFGTPQQQWLDAISVAEAQTLLREGQFGVGSMQPK
                     VEAIVDFINASQQQGKQASGLITSPQTIKAALAHQSGTWITL"
     gene            2107..2496
                     /locus_tag="PN977_000558"
     CDS             2107..2496
                     /locus_tag="PN977_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000214032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA amidotransferase"
                     /protein_id="HDI6040632.1"
                     /translation="MTTTLLAVNGTLMRGLELNPNMQKAGGIFVREDRTDAHYRLWSI
                     NDRHPGMIRVNEGGTHVDVEIWQLPLASFAALLMSEPAGLAIGKIKLADGSEVLGVLA
                     ENWLTEGQREITELGSWRKYTGHFHTV"
     gene            2522..3745
                     /locus_tag="PN977_000559"
     CDS             2522..3745
                     /locus_tag="PN977_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020234945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine permease"
                     /protein_id="HDI6040633.1"
                     /translation="MRKKEENLNTASGLRIAMILLGIAVTPVLLSSSSLGNQLSSGSL
                     ISVVLLGGVILTLLSAITISVGEKARLPTYGIVKYSFGEKGAIAINILMAISLFGWIA
                     VTANMFGHSVHDLLAQHGLEVPLALLVAAGCVIFVASTAFGFAVLGKIAQVAVPVIAL
                     VLCYILYVATHTEVAVPAAIVEMNTGVAVSTVVGTIIVLVATLPDFGSFVHNRKHALI
                     AAGVTFLVAYPLLYWAGATPSAISGQGSLLGAMAVFGAVLPAALLLIFACVTGNAGNM
                     FQGTLVVSTLLTRFPKWQITVALGILSAIVGSMDIMAWFIPFLLFLGIATPPVAGIYI
                     ADFFFYRRNGYQESVLAQESQIKVLTFAAWIIGAAVGFMTVKGLFTLTTIPSVDSILV
                     ACIAYAILSRASQHR"
     gene            3823..4719
                     /locus_tag="PN977_000560"
     CDS             3823..4719
                     /locus_tag="PN977_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000470721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDI6040634.1"
                     /translation="MFISDETLRVIHLVARHQSITTAAEQLNKVPSAISYTIKKAEES
                     LGVELFIRKGRYIELSPAGSYFIEHSKTILGDLEALKRNTVLIHDGIERELTIAVNNI
                     IPGDLLVAFIRDFERQFTSTTLTVDLEVYNGCWDALYSKRADLVYGAPHAVPSSEGII
                     SEPVGQMEWDFVVSPLHPLAAKRHPLENSELRHYPAVCIRDTSVNFPPMQAWLLEGQK
                     PIFVPDFATAIALIEQNVGIGYIPHHLALPLLNSGKLLKKPMREHKHATKLFLAARSD
                     GMGKACQWCIEYLRNPQLIFCS"
     gene            complement(4813..7074)
                     /gene="metE"
                     /locus_tag="PN977_000561"
     CDS             complement(4813..7074)
                     /gene="metE"
                     /locus_tag="PN977_000561"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HDI6040635.1"
                     /translation="MTILNHTLGFPRVGLRRELKKAQESYWAGNSTREELLAVGRELR
                     ARHWEQQKQAGIDLLPVGDFAWYDHVLTTSLLLGNVPARHQNNDGSVDIDTLFRIGRG
                     RAPTGEPAAAAEMTKWFNTNYHYMVPEFVKGQQFKLTWTQLLEEVDEALALGHKVKPV
                     LLGPVTYLWLGKVKGEQFDRLSLLNDILPVYQQVLAELAKRGIEWVQIDEPALVLELP
                     QAWLDAYKPAYDALQGQVKLLLTTYFEGVTPNLDTITALPVQGLHVDLVHGKDDVAEL
                     HKRLPSDWLLSAGLINGRNVWRADLTEKYAQIKDIVGKRDLWVASSCSLLHSPIDLSV
                     ETRLDAEVKSWFAFALQKCHELALLRDALNSGDTAALAAWSAPIQARRHSTRVHNPAV
                     EKRLAAITAQDSQRANVYEVRAEAQRARFKLPAWPTTTIGSFPQTTEIRTLRLDFKKG
                     NLDANNYRTGIAEHIKQAIVEQERLGLDVLVHGEAERNDMVEYFGEHLDGFVFTQNGW
                     VQSYGSRCVKPPIVIGDVSRPAPITVEWAKYAQSLTDKPVKGMLTGPVTILCWSFPRE
                     DVSRETIAKQIALALRDEVADLEAAGIGIIQIDEPALREGLPLRRSDWDAYLQWGVEA
                     FRINAAVAKDDTQIHTHMCYCEFNDIMDSIAALDADVITIETSRSDMELLESFEEFDY
                     PNEIGPGVYDIHSPNVPSVEWIEALLKKAAKRIPAERLWVNPDCGLKTRGWPETRAAL
                     ANMVQAAQNLRRG"
     gene            7192..8145
                     /gene="metR"
                     /locus_tag="PN977_000562"
     CDS             7192..8145
                     /gene="metR"
                     /locus_tag="PN977_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MetR"
                     /protein_id="HDI6040636.1"
                     /translation="MIEVKHLKTLQALRNCGSLAAAAATLHQTQSALSHQFSDLEQRL
                     GFRLFVRKSQPLRFTPQGEILLQLANQVLPQISQALQACNEPQQTRLRIAIECHSCIQ
                     WLTPALENFHKNWPQVEMDFKSGVTFDPQPALQQGELDLVMTSDILPRSGLHYSPMFD
                     YEVRLVLAPDHPLAAKTRITPEDLASETLLIYPVQRSRLDVWRHFLQPAGVSPSLKSV
                     DNTLLLIQMVAARMGIAALPHWVVESFERQGLVVTKTLGEGLWSRLYAAVRDGEQRQP
                     ITEAFIRSARNHACDHLPFVKSAERPTYDAPTVRPGSPARL"
     gene            complement(8033..8932)
                     /gene="bioP"
                     /locus_tag="PN977_000563"
     CDS             complement(8033..8932)
                     /gene="bioP"
                     /locus_tag="PN977_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter"
                     /protein_id="HDI6040637.1"
                     /translation="MALLIITTILWAFSFSFYGEYLAGHVDSYFAVLVRVGLAALVFL
                     PFLRTRGNSLKTVGLYMLVGAMQLGVMYMLSFRAYLYLTVSELLLFTVLTPLYITLIY
                     DIMSKRRLRWGYAFSALLAVIGAGIIRYDQVTDHFWTGLLLVQLSNITFAIGMVGYKR
                     LMETRPMPQHNAFAWFYLGAFLVAVIAWFLLGNAQKMPQTTLQWGILVFLGVVASGIG
                     YFMWNYGATQVDAGTLGIMNNMHVPAGLLVNLAIWHQQPHWPTFITGALVILASLWVH
                     RKWVAPRSSQTADDRRRDCALNE"
     gene            complement(9008..9808)
                     /gene="yigL"
                     /locus_tag="PN977_000564"
     CDS             complement(9008..9808)
                     /gene="yigL"
                     /locus_tag="PN977_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar/pyridoxal phosphate phosphatase YigL"
                     /protein_id="HDI6040638.1"
                     /translation="MYQVVASDLDGTLLSPDHTLSPYAKETLKLLTARGINFVFATGR
                     HHVDVGQIRDNLEIKSYMITSNGARVHDLDGNLIFAHNLDRDIASDLFGVVNDNPDII
                     TNVYRDDEWFMNRHRPEEMRFFKEAVFKYALYEPGLLEPEGVSKVFFTCDSHEQLLPL
                     EQAINARWGDRVNVSFSTLTCLEVMAGGVSKGHALEAVAKKLGYSLQDCIAFGDGMND
                     AEMLSMAGKGCIMGSAHQRLKDLHPELEVIGTNAEDAVPHYLRKLYLS"
     gene            complement(9816..10838)
                     /gene="pldB"
                     /locus_tag="PN977_000565"
     CDS             complement(9816..10838)
                     /gene="pldB"
                     /locus_tag="PN977_000565"
                     /EC_number="3.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipase L2"
                     /protein_id="HDI6040639.1"
                     /translation="MFQQQKDWETRENAFAAFTMGPLTDFWRQRDEAEFTGVDDIPVR
                     FVRFRAQHHDRVVVICPGRIESYVKYAELAYDLFHLGFDVLIIDHRGQGRSGRLLADP
                     HLGHVNRFNDYVDDLAAFWQQEVQPGPWRKRYILAHSMGGAISTLFLQRHPGVCDAIA
                     LTAPMFGIVIRMPSFMARQILNWAEAHPRFRDGYAIGTGRWRALPFAINVLTHSRQRY
                     RRNLRFYADDPTIRVGGPTYHWVRESILAGEQVLAGAGDDATPTLLLQAEEERVVDNR
                     MHDRFCELRTAAGHPVEGGRPLVIKGAYHEILFEKDAMRSVALHAIVDFFNRHNSPSG
                     NRSTEV"
     gene            10949..11569
                     /gene="rhtB"
                     /locus_tag="PN977_000566"
     CDS             10949..11569
                     /gene="rhtB"
                     /locus_tag="PN977_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine/homoserine lactone efflux protein"
                     /protein_id="HDI6040640.1"
                     /translation="MTLEWWFAYLLTSIILSLSPGSGAINTMTTSLNHGYRGAVASIA
                     GLQTGLAIHIVLVGVGLGTLFSRSVIAFEVLKWAGAAYLIWLGIQQWRAAGAIDLKSP
                     ASTQSRRHLFQRAVFVNLTNPKSIVFLAALFPQFIMPQQPQLMQYIVLGVTTIVVDII
                     VMIGYATLAQRIALWIKGPKQMKALNKIFGSLFMLVGALLASARHA"
     gene            complement(11631..12251)
                     /gene="rhtC"
                     /locus_tag="PN977_000567"
     CDS             complement(11631..12251)
                     /gene="rhtC"
                     /locus_tag="PN977_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709629.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine export protein RhtC"
                     /protein_id="HDI6040641.1"
                     /translation="MLMLFLTVAMVHIVALMSPGPDFFFVSQTAVSRSRKEAMMGVLG
                     ITCGVMVWAGIALLGLHLIIEKMAWLHTLIMVGGGLYLCWMGYQMLRGALKKEAVSAP
                     APQVELAKSGRSFLKGLLTNLANPKAIIYFGSVFSLFVGDNVGTTERWGIFALIIIET
                     LAWFTVVASLFALPQMRRGYQRLAKWIDGFAGALFAGFGIHLIISR"
     gene            complement(12315..14144)
                     /gene="recQ"
                     /locus_tag="PN977_000568"
     CDS             complement(12315..14144)
                     /gene="recQ"
                     /locus_tag="PN977_000568"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026263.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HDI6040642.1"
                     /translation="MAQAEVLNLESGAKQVLQETFGYQQFRPGQEEIIDTVLSGRDCL
                     VVMPTGGGKSLCYQIPALLLNGLTVVVSPLISLMKDQVDQLQANGVAAVCLNSTQTRE
                     QQLEVMTGCRTGQIRLLYIAPERLMLDNFLEHLAHWNPVLLAVDEAHCISQWGHDFRP
                     EYAALGQLRQRFPTLPFMALTATADDTTRQDIVRLLGLNDPLIQISSFDRPNIRYMLM
                     EKFKPLDQLMRYVQEQRGKSGIIYCNSRAKVEDTAARLQSKGISAAAYHAGLENNVRA
                     DVQEKFQRDDLQIVVATVAFGMGINKPNVRFVVHFDIPRNIESYYQETGRAGRDGLPA
                     EAMLFYDPADMAWLRRCLEEKPQGQLQDIERHKLNAMGAFAEAQTCRRLVLLNYFGEG
                     RQEPCGNCDICLDPPKQYDGSTDAQIALSTIGRVNQRFGMGYVVEVIRGANNQRIRDY
                     GHDKLKVYGMGRDKSHEHWVSVIRQLIHLGLVTQNIAQHSALQLTEAARPVLRGESSL
                     QLAVPRIVALKPKAMQKSFGGNYDRKLFAKLRKLRKSIADESNVPPYVVFNDATLIEM
                     AEQMPITASEMLSVNGVGMRKLERFGKPFMALIRAHVDGDDEE"
     gene            complement(14269..15138)
                     /gene="pldA"
                     /locus_tag="PN977_000569"
     CDS             complement(14269..15138)
                     /gene="pldA"
                     /locus_tag="PN977_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase A"
                     /protein_id="HDI6040643.1"
                     /translation="MRTLQGWLLPVFMLPMAVYAQEATVKEVHDAPAVRGSIIANMLQ
                     EHDNPFTLYPYDTNYIIYTQTSDLNKEAIASYDWAENARKDEVKFQLSLAFPLWRGIL
                     GPNSVLGASYTQKSWWQLSNSEESSPFRETNYEPQLFLGFATDYRFAGWTLRDVEMGY
                     NHDSNGRSDPTSRSWNRLYTRLMAENGNWLVEVKPWYVVGNTDDNPDITKYMGYYQLK
                     IGYHLGDAVLSAKGQYNWNTGYGGAELGLSYPITKHVRLYTQVYSGYGESLIDYNFNQ
                     TRVGVGVMLNDLF"
     gene            15303..15770
                     /locus_tag="PN977_000570"
     CDS             15303..15770
                     /locus_tag="PN977_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HDI6040644.1"
                     /translation="MSAVLTAEQALKLVGEMFVYHMPFNRALGMELERYEKEFAQLAF
                     KNQPMMVGNWAQSILHGGVIASALDVAAGLVCVGSTLTRHETISEDELRQRLSRMGTI
                     DLRVDYLRPGRGERFTATSSLLRAGNKVAVARVELHNEEQLYIASATATYMVG"
     gene            15822..16712
                     /gene="rarD"
                     /locus_tag="PN977_000571"
     CDS             15822..16712
                     /gene="rarD"
                     /locus_tag="PN977_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HDI6040645.1"
                     /translation="MDAKQTRQGVLLALAAYFIWGIAPAYFKLIYYVPADEILTHRVI
                     WSFFFMVVLMSICRQWSYLKTLIQTPQKIFMLAVSAVLIGGNWLLFIWAVNNHHMLEA
                     SLGYFINPLVNIVLGMIFLGERFRRMQWLAVILAICGVLVQLWTFGSLPIIALGLAFS
                     FAFYGLVRKKIAVEAQTGMLIETMWLLPVAAIYLFAIADSSTSHMGQNPMSLNLLLIA
                     AGIVTTVPLLCFTAAATRLRLSTLGFFQYIGPTLMFLLAVTFYGEKPGADKMVTFAFI
                     WVALAIFVMDAIYTQRRTSK"
     gene            17022..17870
                     /locus_tag="PN977_000572"
     CDS             17022..17870
                     /locus_tag="PN977_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3289 family protein"
                     /protein_id="HDI6040646.1"
                     /translation="MNNILGSSINMPCTLFETITLFDDFSADDMQYGDMEEQDFLSLG
                     LSDISAKVDPYRLIKYHFPGPNSTYGAFSVPTSGTKISQSECIDILFAEMKDLAKMFS
                     FFGQYKTLIQDLIDHFRYGNGNSFHSQELNLSFHERINKYDYNSPIRVIKECIENDIS
                     STPTIGYRPLLLQKIKTELLSSRLNKFNDFKDNFNGMGISIHDISAQKISLLNFQKYP
                     MGWSATIHFIAQDHFGLDVTDIKNKIYNKYRFFRIWFFLQRHKDFAFKPFFTNFNTIE
                     RIENYL"
     gene            17867..18364
                     /locus_tag="PN977_000573"
     CDS             17867..18364
                     /locus_tag="PN977_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000823788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF943 family protein"
                     /protein_id="HDI6040647.1"
                     /translation="MKRKLQKIILYLFLLCCIYNLWTLRPVQILYTYSDAGNSVFLVV
                     DHLPWTDSDKINWYLKHQNEIKNQHPLPEGSWHTWYVIDIGNGFTDYKKYIEGPYEDL
                     YCFPTIKSNDNCIVKNYLMVINEYPYRNTHFFIADNNEYQLTQENKIERVFNPHDFEK
                     DNFQQ"
     gene            complement(18400..19350)
                     /gene="corA"
                     /locus_tag="PN977_000574"
     CDS             complement(18400..19350)
                     /gene="corA"
                     /locus_tag="PN977_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HDI6040648.1"
                     /translation="MLSAFQLENNRLTRLEVEESQPLVNAVWIDLVEPDDDERLRVQS
                     ELGQSLATRPELEDIEASARFFEDDDGLHIHSFFFFEDAEDHAGNSTVAFTIRDGRLF
                     TLRERELPAFRLYRMRARSQSMVDGNAYELLLDLFETKIEQLADEIENIYSDLEQLSR
                     VIMEGHQGDEYDEALSTLAELEDIGWKVRLCLMDTQRALNFLVRKARLPGGQLEQARE
                     ILRDIESLLPHNESLFQKVNFLMQAAMGFINIEQNRIIKIFSVVSVVFLPPTLVASSY
                     GMNFEFMPELKWSFGYPGAIIFMILAGLAPYLYFKRKNWL"
     gene            19720..20484
                     /locus_tag="PN977_000575"
     CDS             19720..20484
                     /locus_tag="PN977_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HDI6040649.1"
                     /translation="MAHRLLIGKGMITLNLKRIFLTFTLLPLFAVAADDCALSDPTLT
                     VQAYTVNPQTERVKMYWQKANGEAWGTLHALLADMNSQGQVQMAMNGGIYDESYAPLG
                     LYIENGQQKVALNLASGEGNFFIRPGGVFYVAGDKVGIVRLDAFKTSKEIQFAVQSGP
                     MLLENGVINPRIHPNVASRKIRNGVGINKQGNAVFLLSQQATNFYDFACYAKAKLNVE
                     QLLYLDGTISHMYMKGWAIPWQHYPFVTMISVEWKG"
     gene            20567..21469
                     /locus_tag="PN977_000576"
     CDS             20567..21469
                     /locus_tag="PN977_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosyl-l-methionine hydroxide
                     adenosyltransferase family protein"
                     /protein_id="HDI6040650.1"
                     /translation="MKKTLATLFLLTCLGSSSAYADNALILQTDFSLKDGAVSAMKGV
                     AFGVDHNLKIFDLTHEIPPYNIWEGAYRLYQTASYWPQGSVFVSVVDPGVGTDRKSVV
                     LKTKNGQYFVSPDNGTLTLVAQTLGIDSVREIDEKANRLKGSEKSYTFHGRDVYAYTG
                     ARLASGAITFEQVGPELPAKVVELSYQKAKATKGEVKGNIPILDIQYGNVWSNISDEL
                     LNQAGIKLNDTLCVTISEGSQQKYAGKMPYVASFGDVPEGQPMVYLNSLLNVSVALNM
                     DNFAQKHQVASGADWNIDVKKCAK"
     gene            complement(21524..23686)
                     /gene="uvrD"
                     /locus_tag="PN977_000577"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
     CDS             complement(21524..23686)
                     /gene="uvrD"
                     /locus_tag="PN977_000577"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase II"
                     /protein_id="HDI6040651.1"
                     /translation="MDVSYLLDSLNDKQREAVAAPRSNLLVLAGAGSGKTRVLVHRIA
                     WLMSVENCSPYSIMAVTFTNKAAAEMRHRIGQLMGTSQGGMWVGTFHGLAHRLLRAHH
                     MDANLPQDFQILDSEDQLRLLKRLIKAMNLDEKQWPPRQAMWYINSQKDEGLRPHHIQ
                     SYGNPVEQTWQKVYQAYQEACDRAGLVDFAELLLRAHELWLNKPHILQHYRERFTNIL
                     VDEFQDTNNIQYAWIRLLAGDTGKVMIVGDDDQSIYGWRGAQVENIQRFLNDFPGAET
                     IRLEQNYRSTSNILSAANALIENNNGRLGKKLWTDGADGEPISLYCAFNELDEARFVV
                     NRIKTWQDNGGALAECAILYRSNAQSRVLEEALLQASMPYRIYGGMRFFERQEIKDAL
                     SYLRLIANRNDDAAFERVVNTPTRGIGDRTLDVVRQTSRDRQLTLWQACRELLQEKAL
                     AGRAASALQRFMELIDALAQETADMPLHVQTDRVIKDSGLRTMYEQEKGEKGQTRIEN
                     LEELVTATRQFSYNEEDEDLMPLQAFLSHAALEAGEGQADTWQDAVQLMTLHSAKGLE
                     FPQVFIVGMEEGMFPSQMSLDEGGRLEEERRLAYVGVTRAMQKLTLTYAETRRLYGKE
                     VYHRPSRFIGELPEECVEEVRLRATVSRPVSHQRMGTPMVENDSGYKLGQRVRHAKFG
                     EGTIVNMEGSGEHSRLQVAFQGQGIKWLVAAYARLETV"
     gene            complement(23770..24486)
                     /gene="yigB"
                     /locus_tag="PN977_000578"
     CDS             complement(23770..24486)
                     /gene="yigB"
                     /locus_tag="PN977_000578"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase YigB"
                     /protein_id="HDI6040652.1"
                     /translation="MRFYRPLGRISALTFDLDDTLYDNRPVILRTEREALTFVQNYHP
                     ALRSFQNEDLQRLRQAVREAEPEIYHDVTRWRFRSIEQAMLDAGLSAEEASAGAHAAM
                     INFAKWRSRIDVPQQTHDTLKQLAKKWPLVAITNGNAQPELFGLGDYFEFVLRAGPHG
                     RSKPFSDMYFLAAEKLNVPIGEILHVGDDLTTDVGGAIRSGMQACWIRPENGDLMQTW
                     DSRLLPHLEISRLASLTSLI"
     gene            complement(24486..25382)
                     /gene="xerC"
                     /locus_tag="PN977_000579"
     CDS             complement(24486..25382)
                     /gene="xerC"
                     /locus_tag="PN977_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HDI6040653.1"
                     /translation="MTDLHTDVERYLRYLSVERQLSPITLLNYQRQLEAIINFASENG
                     LQSWQQCDVTMVRNFAVRSRRKGLGAASLALRLSALRSFFDWLVSQNELKANPAKGVS
                     APKAPRHLPKNIDVDDMNRLLDIDINDPLAVRDRAMLEVMYGAGLRLSELVGLDIKHL
                     DLESGEVWVMGKGSKERRLPIGRNAVAWIEHWLDLRDLFGSEDDALFLSKLGKRISAR
                     NVQKRFAEWGIKQGLNNHVHPHKLRHSFATHMLESSGDLRGVQELLGHANLSTTQIYT
                     HLDFQHLASVYDAAHPRAKRGK"
     gene            complement(25379..26086)
                     /locus_tag="PN977_000580"
     CDS             complement(25379..26086)
                     /locus_tag="PN977_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF484 domain-containing protein"
                     /protein_id="HDI6040654.1"
                     /translation="MKQPGEELQETLTELDDRAVVDYLIKNPEFFIRNARAVEAIRVP
                     HPVRGTVSLVEWHMARARNHIHVLEENMALLMEQAIANEGLFYRLLYLQRSLTAASSL
                     DDMLMRFHRWARDLGLAGASLRLFPDRWRLGAPSNHTHLALSRQSFEPLRIQRLGQEQ
                     HYLGPLNGPELLVVLPEAKAVGSVAMSMLGSDADLGVVLFTSRDASHYQQGQGTQLLH
                     EIALMLPELLERWIERV"
     gene            complement(26083..26907)
                     /gene="dapF"
                     /locus_tag="PN977_000581"
     CDS             complement(26083..26907)
                     /gene="dapF"
                     /locus_tag="PN977_000581"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HDI6040655.1"
                     /translation="MQFSKMHGLGNDFMVVDAVTQNVFFSPELIRRLADRHLGVGFDQ
                     LLVVEPPYDPELDFHYRIFNADGSEVAQCGNGARCFARFVRLKGLTNKRDIRVSTANG
                     RMVLTVTDDDLVRVNMGEPNFEPSAVPFRANKAEKTYIMRAAEQTILCGVVSMGNPHC
                     VIQVDDVDTAAVETLGPVLESHERFPERANIGFMQVVKREHIRLRVYERGAGETQACG
                     SGACAAVAVGIQQGLLAEEVRVELPGGRLDIAWKGPGHPLYMTGPAVHVYDGFIHL"
     gene            complement(26944..27147)
                     /locus_tag="PN977_000582"
     CDS             complement(26944..27147)
                     /locus_tag="PN977_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HDI6040656.1"
                     /translation="MKNVFKALTVLLTLFSLTGCGLKGPLYFPPADKNAPPPTKPVET
                     QTQSTVPDKNDRATGDGPSQVNY"
     gene            27306..27491
                     /locus_tag="PN977_000583"
                     /pseudo
     CDS             27306..27491
                     /locus_tag="PN977_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000580503.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DKNYY family protein"
     gene            27617..27937
                     /gene="cyaY"
                     /locus_tag="PN977_000584"
     CDS             27617..27937
                     /gene="cyaY"
                     /locus_tag="PN977_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000999950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron donor protein CyaY"
                     /protein_id="HDI6040657.1"
                     /translation="MNDSEFHRLADQLWLTIEERLDDWDGDSDIDCEINGGVLTITFE
                     NGSKIIINRQEPLHQVWLATKQGGYHFDMKGDEWICDRSGETFWDLLEQAATQQAGET
                     VSFR"
     gene            complement(27977..30523)
                     /gene="cyaA"
                     /locus_tag="PN977_000585"
     CDS             complement(27977..30523)
                     /gene="cyaA"
                     /locus_tag="PN977_000585"
                     /EC_number="4.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate cyclase"
                     /protein_id="HDI6040658.1"
                     /translation="MYLYIETLKQRLDAINQLRVDRALAAMGPAFQQVYSLLPTLLHY
                     HHPLMPGYLDGNVPKGICLYTPDETQRHYLNELELYRGMSVQDPPKGELPITGVYTMG
                     STSSVGQSCSSDLDIWVCHQSWLDSEERQLLQRKCSLLESWAASLGVEVSFFLIDENR
                     FRHNESGSLGGEDCGSTQHILLLDEFYRTAVRLAGKRILWNMVPCDEEEHYDDYVMTL
                     YAQGVLTPNEWLDLGGLSSLSAEEYFGASLWQLYKSIDSPYKAVLKTLLLEAYSWEYP
                     NPRLLAKDIKQRLHDGEIVSFGLDPYCMMLERVTEYLTAIEDFTRLDLVRRCFYLKVC
                     EKLSRERACVGWRREVLTQLVKEWEWDDARLAMLDNRANWKIDQVREAHNELLDAMMQ
                     SYRNLIRFARRNNLSVSASPQDIGVLTRKLYAAFEALPGKVTLVNPQISPDLSEPNLT
                     FIYVPPGRANRSGWYLYNRAPNIESIISHQPLEYNRYLNKLVAWAWFNGLLTSRTRLY
                     IKGNGIVDLPKLQEMVADVSHHFPLRLPAPTPKALYSPCEIRHLAIIVNLEYDPTAAF
                     RNQVVHFDFRKLDVFSFGENQNCLVGSVDLLYRNSWNEVRTLHFNGEQSMIEALKTIL
                     GKMHQDAAPPDSVEVFCYSQHLRGLIRTRVQQLVSECIELRLSSTRQETGRFKALRVS
                     GQTWGLFFERLNVSVQKLENAIEFYGAISHNKLHGLSVQVETNHVKLPAVVDGFASEG
                     IIQFFFEETQDENGFNIYILDESNRVEVYHHCEGSKEELVRDVSRFYSSSHDRFTYGS
                     SFINFNLPQFYQIVKVDGREQVIPFRTKSIGNMPPANQDHDTPLLQQYFS"
     gene            30910..31851
                     /gene="hemC"
                     /locus_tag="PN977_000586"
     CDS             30910..31851
                     /gene="hemC"
                     /locus_tag="PN977_000586"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HDI6040659.1"
                     /translation="MLDNVLRIATRQSPLALWQAHYVKDKLMASHPGLVVELVPMVTR
                     GDVILDTPLAKVGGKGLFVKELEVALLENRADIAVHSMKDVPVEFPQGLGLVTICERE
                     DPRDAFVSNNYESLDALPAGSIVGTSSLRRQCQLAERRPDLIIRSLRGNVGTRLSKLD
                     NGEYDAIILAVAGLKRLGLESRIRAALPPEISLPAVGQGAVGIECRLDDSRTRELLAA
                     LNHHETALRVTAERAMNTRLEGGCQVPIGSYAELIDGEIWLRALVGAPDGSQIIRGER
                     RGAPQDAEQMGISLAEELLNNGAREILAEVYNGDAPA"
     gene            31848..32588
                     /gene="hemD"
                     /locus_tag="PN977_000587"
     CDS             31848..32588
                     /gene="hemD"
                     /locus_tag="PN977_000587"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HDI6040660.1"
                     /translation="MSILVTRPSPAGEELVSRLRTLGQVAWHFPLIEFSPGRQLPQLA
                     DQLAALGESDLLFALSQHAVAFAQSQLHQQDRKWPRLPDYFAIGRTTALALHTVSGQK
                     ILYPQDREISEVLLQLPELQNIAGKRALILRGNGGRELIGDSLTARGAEVIFCECYQR
                     CAIHYDGAEEAMRWQSREVTTVVVTSGEMLQQLWSLIPQWYREHWLLRCRLLVVSERL
                     AKLARELGWQDIKVADNADNDALLRALQ"
     gene            32610..33803
                     /gene="hemX"
                     /locus_tag="PN977_000588"
     CDS             32610..33803
                     /gene="hemX"
                     /locus_tag="PN977_000588"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HDI6040661.1"
                     /translation="MTEQEKTSAVVEETREAVDTTSQPVATEKKSKNNTALILSAVAI
                     AIALAAGVGLYGWGKQQAVNQTATSDALANQLTALQKAQESQKAELEGIIKQQAVQLE
                     QANRQQETLAKQLDEVQQKVATISGSDAKTWLLAQADFLVKLAGRKLWSDQDVTTAAA
                     LLKSADASLADMNDPSLITVRRAITDDIASLSAVSQVDYDGIILKLNQLSNQVDNLRL
                     ADNDSDGSPMDSDGEELSSSISEWRINLQKSWQNFMDNFITIRRRDDTAVPLLAPNQD
                     IYLRENIRSRLLVAAQAVPRHQEETYRQALENVSTWVRAYYDTDDATTKAFLDEVDQL
                     SQQNISMDLPETLQSQAMLEKLMQTRVRNLLAQPAAGTTEAKPAPAPAPAPQADTPAA
                     APQGE"
     gene            33806..35002
                     /gene="hemY"
                     /locus_tag="PN977_000589"
     CDS             33806..35002
                     /gene="hemY"
                     /locus_tag="PN977_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX biogenesis protein HemY"
                     /protein_id="HDI6040662.1"
                     /translation="MLKVLLLFVLLIAGIVVGPMIAGHQGYVLIQTDNYNIETSVTGL
                     AIILILAMVVLFAIEWLLRRIFRTGAHTRGWFVGRKRRRARKQTEQALLKLAEGDYQQ
                     VEKLMAKNADHAEQPVVNYLLAAEAAQQRGDEARANQHLERAAELAGNDTIPVEITRV
                     RLQLARNENHAARHGVDKLLEVTPRHPEVLRLAEQAYIRTGAWSSLLDIIPSMAKAHV
                     GDEEHRAMLEQQAWIGLMDQARADNGSEGLRNWWKNQSRKTRHQVALQVAMAEHLIEC
                     DDHDTAQQIIIDGLKRQYDDRLLLPIPRLKTNNPEQLEKVLRQQIKNVGDRPLLWSTL
                     GQSLMKHGEWQEASLAFRAALKQRPDAYDYAWLADALDRLHKPEEAAAMRRDGLMLTL
                     QNNPSQ"
     gene            35666..37321
                     /gene="aslA"
                     /locus_tag="PN977_000590"
     CDS             35666..37321
                     /gene="aslA"
                     /locus_tag="PN977_000590"
                     /EC_number="3.1.6.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase AslA"
                     /protein_id="HDI6040663.1"
                     /translation="MEFSFSPKLLVVAVAAALPLMANAADTPSTATARKGFAGYDHPN
                     QYLVKPATTIADNMMPVMQHPAQDKETQQKLAELEKKTGKKPNVVVFLLDDVGWMDVG
                     FNGGGVAVGNPTPDIDAVASQGLILTSAYSQPSSSPTRATILTGQYSIHHGILMPPMY
                     GQPGGLQGLTTLPQLLHDQGYVTQAIGKWHMGENKESQPQNVGFDDFRGFNSVSDMYT
                     EWRDVHVNPEVALSPDRSEYIQKLPFSKDDVHAVRGGEQEAIADITPKYMEDLDQRWM
                     EYGVKFLDKMAKSDKPFFLYYGTRGCHFDNYPNAKYAGSSPARTSYGDCMVEMNDIFA
                     NLYKALEKNGQLDNTLIVFTSDNGPEAEVPPHGRTPFRGAKGSTWEGGVRVPTFVYWK
                     GMIQPRKSDGIVDLADLFPTALDLAGHPGAKVANLVPKTTFIDGVDQTSFFLGTNGQS
                     NRKAEHYFLNGKLSAVRMDEFKYHVLIQQPYAYTQSGYQGGFTGTVMQTAGSSVFNLY
                     TDPQESDSIGVRHIPMGVPLQTEMHAYMEILKKYPPRAQIKSD"
     gene            complement(37479..38714)
                     /locus_tag="PN977_000591"
     CDS             complement(37479..38714)
                     /locus_tag="PN977_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709601.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HDI6040664.1"
                     /translation="MLQQVPTRAFHVMAKPSGSDCNLNCDYYFYLEKQSLYREKPVTH
                     MDDDTLEAYVRHYIAASETQNEVAFTWQGGEPTLLGLDFYRRAVALQAKYGAGRKISN
                     SFQTNGVLLDDEWCAFLAENHFLVGLSLDGPAEIHNQYRVTKGGRPTHKLVMRALTLL
                     QKHHVNYNVLVCVNRTSALQPLQVYDFLCDAGVEFIQFIPVVERLADETAVHAGLKLH
                     APGDIQGELTEWSVRPDEFGEFLVAIFDHWIKRDVGKIFVMNIEWAFANFVGAPGAVC
                     HHQPTCGRSVIVEHNGDVYACDHYVYSQYRLGNMLQQTIAEMVDSPQQQVFGEDKFKQ
                     LPAQCRSCNVLKACWGGCPKHRFMLDASGKPGLNYLCAGYQRYFRHLPPYLKAMADLL
                     AHGRPASDIMQAHLMVVNK"
     gene            complement(38861..38937)
                     /locus_tag="PN977_000592"
     tRNA            complement(38861..38937)
                     /locus_tag="PN977_000592"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(38901..38903),aa:Pro,seq:tgg)
     gene            complement(38980..39066)
                     /locus_tag="PN977_000593"
     tRNA            complement(38980..39066)
                     /locus_tag="PN977_000593"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(39030..39032),aa:Leu,seq:cag)
     gene            complement(39087..39162)
                     /locus_tag="PN977_000594"
     tRNA            complement(39087..39162)
                     /locus_tag="PN977_000594"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(39127..39129),aa:His,seq:gtg)
     gene            complement(39221..39297)
                     /locus_tag="PN977_000595"
     tRNA            complement(39221..39297)
                     /locus_tag="PN977_000595"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(39261..39263),aa:Arg,seq:ccg)
     gene            complement(39400..40785)
                     /locus_tag="PN977_000596"
     CDS             complement(39400..40785)
                     /locus_tag="PN977_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDI6040665.1"
                     /translation="MADNKPELQRGLEARHIELIALGGTIGVGLFMGAASTLKWAGPS
                     VLLAYIIAGLFVFFIMRSMGEMLFLEPVTGSFAVYAHRYMSPFFGYLTAWSYWFMWMA
                     VGISEITAIGVYVQFWFPEMAQWIPALIAVALVALANLAAVRLYGEIEFWFAMIKVTT
                     IIVMIVIGLGVIFFGFGNGGQSIGFSNLTEHGGFFAGGWKGFLTALCIVVASYQGVEL
                     IGITAGEAKNPQVTLRSAVGKVLWRILIFYVGAIFVIVTIFPWNEIGSNGSPFVLTFA
                     KIGITAAAGIINFVVLTAALSGCNSGMYSCGRMLYALAKNRQLPAAMAKVSRHGVPVA
                     GVAVSIAILLIGSCLNYIIPNPQRVFVYVYSASVLPGMVPWFVILISQLRFRRAHKAA
                     IASHPFRSILFPWANYVTMAFLICVLIGMYFNEDTRMSLFVGIIFMLAVTAIYKIFGL
                     NRHGKAHKLEE"
     gene            complement(40976..41716)
                     /gene="rffM"
                     /locus_tag="PN977_000597"
     CDS             complement(40976..41716)
                     /gene="rffM"
                     /locus_tag="PN977_000597"
                     /EC_number="2.4.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     N-acetylmannosaminouronosyltransferase"
                     /protein_id="HDI6040666.1"
                     /translation="MNNNTTAPTYTLRGLQLIGWRDMQHALDYLFADGQLKQGTLVAI
                     NAEKMLTIEDNAEVRELINAAEFKYADGISVVRSVRKKYPQAQVSRVAGADLWEELMA
                     RAGKEGTPVFLVGGKPEVLAQTEAKLRNQWNVNIVGSQDGYFKPEQRQALFERIHASG
                     AQIVTVAMGSPKQEIFMRDCRLVHPDALYMGVGGTYDVFTGHVKRAPKIWQTLGLEWL
                     YRLLSQPSRIKRQLRLLRYLRWHYTGNL"
     gene            complement(41719..43071)
                     /gene="wzyE"
                     /locus_tag="PN977_000598"
     CDS             complement(41719..43071)
                     /gene="wzyE"
                     /locus_tag="PN977_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECA oligosaccharide polymerase"
                     /protein_id="HDI6040667.1"
                     /translation="MSLLQFSGLFVVWLLCTLFIATLTWFEFRRVRFNFNVFFSLLFL
                     LTFFFGFPLTSVLVFRFDVGVAPPEILLQALLSAGCFYAVYYVTYKTRLRKRVADAPR
                     RPLFTMNRVETNLTWVILMGIALVSVGIFFMHNGFLLFRLNSYSQIFSSEVSGVALKR
                     FFYFFIPAMLVVYFLRQDSKAWLFFLVSTVAFGLLTYMIVGGTRANIIIAFAIFLFIG
                     IIRGWISLWMLAAAGVLGIVGMFWLALKRYGMNVSGDEAFYTFLYLTRDTFSPWENLA
                     LLLQNYDNIDFQGLAPIVRDFYVFIPSWLWPGRPSMVLNSANYFTWEVLNNHSGLAIS
                     PTLIGSLVVMGGALFIPLGAIVVGLIIKWFDWLYELGNRETNRYKAAILHSFCFGAIF
                     NMIVLAREGLDSFVSRVVFFIVVFGACLMIAKLLYWLFESAGLIHKRTKSSLRTQVEG
                     "
     gene            complement(43068..44147)
                     /gene="rffT"
                     /locus_tag="PN977_000599"
                     /gene_synonym="wecF"
     CDS             complement(43068..44147)
                     /gene="rffT"
                     /locus_tag="PN977_000599"
                     /gene_synonym="wecF"
                     /EC_number="2.4.1.325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDP-N-acetylfucosamine:lipid II
                     N-acetylfucosaminyltransferase"
                     /protein_id="HDI6040668.1"
                     /translation="MTVLIHVLGSDIPHHNRTVLRFFNDALAATSEHAREFMVVGKDD
                     GLSDSCPTLSVQFFPGKKSLAEAVIAKAKANRQQRFFFHGQFNPTLWLALLSGGIKPS
                     QFYWHIWGADLYELSSGLRYKLFYPLRRLAQKRVGCVFATRGDLSFFAKTHPKVRGEL
                     LYFPTRMDPSLNTMANDRQREGKMTILVGNSGDRSNEHIAALRAVHQQFGDTVKVVVP
                     MGYPPNNEAYIEEVRQAGLELFSEENLQVLSEKLEFDAYLTLLRQCDLGYFIFARQQG
                     IGTLCLLIQAGIPCVLNRENPFWQDMTEQHLPVLFTTDDLNEDIVREAQRQLASVDKN
                     TIAFFSPNYLQGWQRALAIATGEVA"
     gene            complement(44144..45394)
                     /gene="wzxE"
                     /locus_tag="PN977_000600"
     CDS             complement(44144..45394)
                     /gene="wzxE"
                     /locus_tag="PN977_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid III flippase WzxE"
                     /protein_id="HDI6040669.1"
                     /translation="MSLAKASLWTAASTLVKIGAGLLVGKLLAVSFGPAGLGLAANFR
                     QLITVLGVLAGAGIFNGVTKYVAQYHDNPQQLRRVVGTSSAMVLGFSMLMALVFVLAA
                     APISQGLFGNTDYQGLVRLVALVQMGIAWGNLLLALMKGFRDAAGNALSLIVGSLIGV
                     LAYYVSYRLGGYEGALLGLALIPALVVIPAVIMLIKRGAIPLSYLKPSWDNGLAGQLS
                     KFTLMALITSVTLPVAYIMMRKLLAAQYSWDEVGIWQGVSSISDAYLQFITASFSVYL
                     LPTLSRLTEKRDITREVVKSLKFVLPAVAAASFSVWLLRDFAIWLLLSNKFTAMRDLF
                     AWQLVGDVLKVGAYVFGYLVIAKASLRFYILAEVSQFTLLMVFSHWLIPAHGALGAAQ
                     AYMATYIVYFSLCCGVFLLWRRRA"
     gene            complement(45396..46526)
                     /gene="rffA"
                     /locus_tag="PN977_000601"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
     CDS             complement(45396..46526)
                     /gene="rffA"
                     /locus_tag="PN977_000601"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
                     /EC_number="2.6.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxygalactose transaminase"
                     /protein_id="HDI6040670.1"
                     /translation="MIPFNAPPVVGTELDYMQSAMGSGKLCGDGGFTRRCQQWLEQRF
                     GSAKVLLTPSCTASLEMAALLLDIQPGDEVIMPSYTFVSTANAFVLRGAKIVFVDVRP
                     DTMNIDETLIEAAITDKTRVIVPVHYAGVACEMDTIMALAKKHNLFVVEDAAQGVMST
                     YKGRALGTIGHIGCFSFHETKNYTAGGEGGATLINDKALIERAEIIREKGTNRSQFFR
                     GQVDKYTWRDIGSSYLMSDLQAAYLWAQLEAAERINQQRLALWQNYYDALAPLAKAGR
                     IELPSIPDGCVQNAHMFYIKLRDIDDRSALINFLKEAEIMAVFHYIPLHGCPAGERFG
                     EFHGEDRYTTKESERLLRLPLFYNLSPVNQRTVIATLLNYFS"
     gene            complement(46531..47205)
                     /gene="rffC"
                     /locus_tag="PN977_000602"
                     /gene_synonym="wecD"
     CDS             complement(46531..47205)
                     /gene="rffC"
                     /locus_tag="PN977_000602"
                     /gene_synonym="wecD"
                     /EC_number="2.3.1.210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxy-D-galactose
                     acyltransferase"
                     /protein_id="HDI6040671.1"
                     /translation="MPVRASIEPLTWENAFFGVNSAIVRITSEAPLLTPDALAPWSRV
                     QAKIAASNTGELDALQQLGFSLVEGEVDLALPVNNVSDSGAVVAQETDIPALRQLASA
                     AFAQSRFRAPWYAPDASGRFYAQWIENAVRGTFDHQCLILRAASGDIRGYVSLRELNA
                     TDARIGLLAGRGAGAELMQTALNWAYARGKTTLRVATQMGNTAALKRYIQSGANVEST
                     TYWLYR"
     gene            complement(47183..48064)
                     /gene="rfbA"
                     /locus_tag="PN977_000603"
     CDS             complement(47183..48064)
                     /gene="rfbA"
                     /locus_tag="PN977_000603"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HDI6040672.1"
                     /translation="MKGIILAGGSGTRLHPITRGVSKQLLPIYDKPMIYYPLSVLMLA
                     GIREILIITTPEDKGYFQRLLGDGSEFGIQLEYAEQPSPDGLAQAFIIGETFLNGEPS
                     CLVLGDNIFFGQGFSPKLRHVAARTEGATVFGYQVMDPERFGVVEFDDNFRAISLEEK
                     PKQPKSNWAVTGLYFYDSKVVEYAKQVKPSERGELEITSINQMYLEAGNLTVELLGRG
                     FAWLDTGTHDSLIEASTFVQTVEKRQGFKIACLEEIAWRNGWLDDEGVKRAASSLAKT
                     GYGQYLLELLRARPRQY"
     gene            complement(48083..49150)
                     /gene="rffG"
                     /locus_tag="PN977_000604"
     CDS             complement(48083..49150)
                     /gene="rffG"
                     /locus_tag="PN977_000604"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HDI6040673.1"
                     /translation="MRKILITGGAGFIGSALVRYIINETSDAVVVVDKLTYAGNLMSL
                     APVAQSERFAFEKVDICDRAELARVFTEHQPDCVMHLAAESHVDRSIDGPAAFIETNI
                     VGTYTLLEAARTYWNALTEDKKSAFRFHHISTDEVYGDLHSTDDFFTETTPYAPSSPY
                     SASKASSDHLVRAWLRTYGLPTLITNCSNNYGPYHFPEKLIPLMILNALAGKPLPVYG
                     NGQQIRDWLYVEDHARALYCVATTGKVGETYNIGGHNERKNLDVVETICELLEELAPN
                     KPHGVAHYRDLITFVADRPGHDLRYAIDASKIARELGWLPQETFESGMRKTVQWYLAN
                     ESWWKQVQDGSYQGERLGLKG"
     gene            complement(49150..50412)
                     /gene="wecC"
                     /locus_tag="PN977_000605"
     CDS             complement(49150..50412)
                     /gene="wecC"
                     /locus_tag="PN977_000605"
                     /EC_number="1.1.1.336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetyl-D-mannosamine dehydrogenase"
                     /protein_id="HDI6040674.1"
                     /translation="MSFATISVIGLGYIGLPTAAAFASRQKQVIGVDINQHAVDTINR
                     GEIHIVEPDLASVVKTAVEGGFLRASTTPVEADAWLIAVPTPFKGDHEPDMTYVESAA
                     RSIAPVLKKGALVILESTSPVGSTEKMAEWLAEMRPDLTFPQQVGELADVNIAYCPER
                     VLPGQVMVELIKNDRVIGGMTPVCSARASELYKIFLEGECVVTNSRTAEMCKLTENSF
                     RDVNIAFANELSLICADQGINVWELIRLANRHPRVNILQPGPGVGGHCIAVDPWFIVA
                     QNPQQARLIRTAREVNDHKPFWVIDQVKAAVADCLAATDKRASELKIACFGLAFKPNI
                     DDLRESPAMEIAELLAQWHSGETLVVEPNIHQLPKKLTGLCTLAQLDEALATADVLVM
                     LVDHSQFKVINGDNVHQQYVVDAKGVWR"
     gene            complement(50409..51539)
                     /gene="wecB"
                     /locus_tag="PN977_000606"
     CDS             complement(50409..51539)
                     /gene="wecB"
                     /locus_tag="PN977_000606"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HDI6040675.1"
                     /translation="MKVLTVFGTRPEAIKMAPLVHALAKDPFFEAKVCVTAQHREMLD
                     QVLKLFSIVPDYDLNIMQPGQGLTEITCRILEGLKPILAEFKPDVVLVHGDTTTTLAT
                     SLAAFYQRIPVGHVEAGLRTGDLYSPWPEEANRTLTGHLAMYHFSPTETSRQNLLREN
                     VADSRIFITGNTVIDALLWVRDQVMSSDTLRSELAANYPFIDPDKKMILVTGHRRESF
                     GRGFEEICQALADIATTHQDIQIVYPVHLNPNVREPVNRILGHVKNVILIDPQEYLPF
                     VWLMNHAWLILTDSGGIQEEAPSLGKPVLVMRDTTERPEAVTAGTVRLVGTDKQRIVE
                     EVTRLLKDENEYQAMSRAHNPYGDGQACSRILEALKNNRISL"
     gene            complement(51595..52641)
                     /gene="wzzE"
                     /locus_tag="PN977_000607"
     CDS             complement(51595..52641)
                     /gene="wzzE"
                     /locus_tag="PN977_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECA polysaccharide chain length modulation
                     protein"
                     /protein_id="HDI6040676.1"
                     /translation="MTQPMPGKPAEDAENELDIRGLFRTLWAGKLWIIGMGLAFALIA
                     LAYTFFARQEWSATAITDRPTVNMLGGYYSQQQFLRNLDVRSNMASADQPSVMDEAYK
                     EFVMQLASWDTRREFWLQTDYYKQRMVGNSKADAALLDEMINNIVFIPGDFTRAVNDS
                     VKLIAETAPDANNLLRQYVAFASQRAASHLNDELKGAWAARTIQMKAQVKRQEEVAKA
                     IYDRRMNSIEQALKIAEQHNISRSATDVPAEELPDSEMFLLGRPMLQARLENLQAVGP
                     AFDLDYDQNRAMLNTLNVGPTLDPRFQTYRYLRTPEEPVKRDSPRRAFLMIMWGIVGG
                     LIGAGVALTRRCSK"
     gene            complement(52653..53756)
                     /gene="wecA"
                     /locus_tag="PN977_000608"
     CDS             complement(52653..53756)
                     /gene="wecA"
                     /locus_tag="PN977_000608"
                     /EC_number="2.7.8.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--undecaprenyl-phosphate
                     N-acetylglucosaminephosphotransferase"
                     /protein_id="HDI6040677.1"
                     /translation="MNLLTVSTDLISIFLFTTLFLFFARKVAKKVGLVDKPNFRKRHQ
                     GLIPLVGGISVYAGICFTFGIVDYYIPHASLYLACAGVLVFIGALDDRFDISVKIRAT
                     IQAAVGIVMMVFGKLYLSSLGYIFGSWEMVLGPFGYFLTLFAVWAAINAFNMVDGIDG
                     LLGGLSCVSFAAIGMILWFDGQTSLAIWCFAMIAAILPYIMLNLGILGRRYKVFMGDA
                     GSTLIGFTVIWILLETTQGKTHPISPVTALWIIAIPLMDMVAIMYRRLRKGMSPFSPD
                     RQHIHHLIMRAGFTSRQAFVLITLAAALLASIGVLAEYSHFVPEWVMLVLFLLAFFLY
                     GYCIKRAWKVARFIKRVKRRLRRNRGGSPNLTK"
     gene            complement(53996..55255)
                     /gene="rho"
                     /locus_tag="PN977_000609"
     CDS             complement(53996..55255)
                     /gene="rho"
                     /locus_tag="PN977_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017492510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HDI6040678.1"
                     /translation="MNLTELKNTPVSELITLGENMGLENLARMRKQDIIFAILKQHAK
                     SGEDIFGDGVLEILQDGFGFLRSADSSYLAGPDDIYVSPSQIRRFNLRTGDTISGKIR
                     PPKEGERYFALLKVNEVNFDKPENARNKILFENLTPLHANSRLRMERGNGSTEDLTAR
                     VLDLASPIGRGQRGLIVAPPKAGKTMLLQNIAQSIAYNHPDCVLMVLLIDERPEEVTE
                     MQRLVKGEVVASTFDEPASRHVQVAEMVIEKAKRLVEHKKDVIILLDSITRLARAYNT
                     VVPASGKVLTGGVDANALHRPKRFFGAARNVEEGGSLTIIATALIDTGSKMDEVIYEE
                     FKGTGNMELHLSRKIAEKRVFPAIDYNRSGTRKEELLTTQEELQKMWILRKIIHPMGE
                     IDAMEFLINKLAMTKTNDDFFEMMKRS"
     gene            complement(55340..55441)
                     /gene="rhoL"
                     /locus_tag="PN977_000610"
     CDS             complement(55340..55441)
                     /gene="rhoL"
                     /locus_tag="PN977_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rho operon leader peptide RhoL"
                     /protein_id="HDI6040679.1"
                     /translation="MRSEQISGSSLNPSCRFSSAYSPVTRQRKDMSR"
     gene            complement(55582..55911)
                     /gene="trxA"
                     /locus_tag="PN977_000611"
     CDS             complement(55582..55911)
                     /gene="trxA"
                     /locus_tag="PN977_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017375425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxA"
                     /protein_id="HDI6040680.1"
                     /translation="MSDKIIHLTDDSFDTDVLKADGAILVDFWAEWCGPCKMIAPILD
                     EIADEYQGKLTVAKLNIDQNPGTAPKYGIRGIPTLLLFKNGEVAATKVGALSKGQLKE
                     FLDANLA"
     gene            56042..57307
                     /gene="rhlB"
                     /locus_tag="PN977_000612"
     CDS             56042..57307
                     /gene="rhlB"
                     /locus_tag="PN977_000612"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlB"
                     /protein_id="HDI6040681.1"
                     /translation="MSKTHLTEQKFSDFALHPKVVEALEKKGFHNCTPIQALALPLTL
                     AGRDVAGQAQTGTGKTMAFLTSTFHYLLSHPAIADRKVNQPRALIMAPTRELAVQIHA
                     DAEPLAQATGLKLGLAYGGDGYDKQLKVLESGVDILIGTTGRLIDYAKQNHINLGAIQ
                     VVVLDEADRMYDLGFIKDIRWLFRRMPPANQRLNMLFSATLSYRVRELAFEQMNNAEY
                     IEVEPEQKTGHRIKEELFYPSNEEKMRLLQTLIEEEWPDRAIIFANTKHRCEEIWGHL
                     AADGHRVGLLTGDVAQKKRLRILDEFTRGDLDILVATDVAARGLHIPAVTHVFNYDLP
                     DDCEDYVHRIGRTGRAGASGHSISLACEEYALNLPAIETYIGHSIPVSKYNPDALMTD
                     LPKPLRLTRPRTGNGPRRTGAPRNRRRSG"
     gene            57443..58927
                     /gene="gppA"
                     /locus_tag="PN977_000613"
     CDS             57443..58927
                     /gene="gppA"
                     /locus_tag="PN977_000613"
                     /EC_number="3.6.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanosine-5'-triphosphate,3'-diphosphate
                     diphosphatase"
                     /protein_id="HDI6040682.1"
                     /translation="MGSTSSLYAAIDLGSNSFHMLVVREVAGSIQTLTRIKRKVRLAA
                     GLNSENALSNEAMERGWQCLRLFAERLQDIPPSQIRVVATATLRLAVNAGDFIAKAQE
                     ILGCPVQVISGEEEARLIYQGVAHTTGGADQRLVVDIGGASTELVTGTGAQTTSLFSL
                     SMGCVTWLERYFADRNLGQENFDAAEKAAREVLRPVADELRYHGWKVCVGASGTVQAL
                     QEIMMAQGMDERITLEKLQQLKQRAIHCGRLEELEIDGLTLERALVFPSGLAILIAIF
                     TELNIQCMTLAGGALREGLVYGMLHLTVEQDIRSRTLRNIQRRFMIDIDQAQRVAKVA
                     ANFFDQVENEWHLEAISRDLLISACQLHEIGLSVDFKQAPQHAAYLVRNLDLPGFTPA
                     QKKLLATLLLNQTNPVDLSSLHQQNAVPPRVAEQLCRLLRLAIIFASRRRDDLVPEMT
                     LQANHELLTLTLPQGWLTQHPLGKEIIDQESQWQSYVHWPLEVH"
     gene            complement(58974..60995)
                     /gene="rep"
                     /locus_tag="PN977_000614"
     CDS             complement(58974..60995)
                     /gene="rep"
                     /locus_tag="PN977_000614"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase Rep"
                     /protein_id="HDI6040683.1"
                     /translation="MRLNPGQQQAVEFVTGPCLVLAGAGSGKTRVITNKIAHLIRGCG
                     YQARHIAAVTFTNKAAREMKERVGQTLGRKEARGLMISTFHTLGLDIIKREYAALGMK
                     ANFSLFDDTDQLALLKELTEGLIEDDKVLLQQLISTISNWKNDLKTPSQAAASAIGER
                     DRIFAHCYGLYDAHLKACNVLDFDDLILLPTLLLQRNEEVRERWQNKIRYLLVDEYQD
                     TNTSQYELVKLLVGSRARFTVVGDDDQSIYSWRGARPQNLVLLSQDFPALKVIKLEQN
                     YRSSGRILKAANILIANNPHVFEKRLFSELGYGAELKVLSANNEEHEAERVTGELIAH
                     HFVNKTQYKDYAILYRGNHQSRVFEKFLMQNRIPYKISGGTSFFSRPEIKDLLAYLRV
                     LTNPDDDSAFLRIVNTPKREIGPATLKKLGEWAMTRNKSMFTASFDMGLSQTLSGRGY
                     EALTRFTHWLAEIQRLAEREPIAAVRDLIHGMDYESWLYETSPSPKAAEMRMKNVNQL
                     FSWMTEMLEGSELDEPMTLTQVVTRFTLRDMMERGESEEELDQVQLMTLHASKGLEFP
                     YVYMVGMEEGFLPHQSSIDEDNIDEERRLAYVGITRAQKELTFTLCKERRQYGELVRP
                     EPSRFLLELPQDDLIWEQERKVVSAEERMQKGQSHLANLKAMMAAKRGK"
     gene            61213..61662
                     /locus_tag="PN977_000615"
     CDS             61213..61662
                     /locus_tag="PN977_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HDI6040684.1"
                     /translation="MAINFSPKVGEILECNFGNYPVSQNGQFSTTYYDGRIPPEMIKN
                     RLVVVLNGKINGNACIVVPLSTTRDHDKLNRGMHVEIASNVINDLQFFDQQIRWAKAD
                     LVQQVSRNRLNRARTYRGYLNQCLPHELVADIQRAVIKSINAISLIN"
     gene            61815..62096
                     /gene="ppiC"
                     /locus_tag="PN977_000616"
     CDS             61815..62096
                     /gene="ppiC"
                     /locus_tag="PN977_000616"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004097481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase PpiC"
                     /protein_id="HDI6040685.1"
                     /translation="MAKTAAALHILVKEEKLALELLEQIKNGADFGKLAKKHSICPSG
                     KRGGDLGEFRQGQMVPAFDKVVFSCPVLEPTGPLHTQFGYHIIKVLYRN"
     gene            complement(62142..63617)
                     /gene="ilvC"
                     /locus_tag="PN977_000617"
     CDS             complement(62142..63617)
                     /gene="ilvC"
                     /locus_tag="PN977_000617"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HDI6040686.1"
                     /translation="MANYFNTLNLRQQLAQLGKCRFMGRDEFADGASYLQGKKVVIVG
                     CGAQGLNQGLNMRDSGLDISYALRKEAIAEKRASWRKATENGFKVGTYEELIPQADLV
                     VNLTPDKQHSDVVRTVQPLMKDGAALGYSHGFNIVEVGEQIRKDITVVMVAPKCPGTE
                     VREEYKRGFGVPTLIAVHPENDPKGEGMAIAKAWAAATGGHRAGVLESSFVAEVKSDL
                     MGEQTILCGMLQAGSLLCFDKLVEEGTDPAYAEKLIQFGWETITEALKQGGITLMMDR
                     LSNPAKLRAYALSEQLKEIMAPLFQKHMDDIISGEFSSGMMADWANDDKKLLTWREET
                     GKTAFETAPQYEGKIGEQEYFDKGVLMIAMVKAGVELAFETMVDSGIIEESAYYESLH
                     ELPLIANTIARKRLYEMNVVISDTAEYGNYLFSYACVPLLKPFMAELQPGDLGKAIPE
                     GAVDNAQLRDVNEAIRSHAIEQVGKKLRGYMTDMKRIAVAG"
     gene            63767..64657
                     /gene="ilvY"
                     /locus_tag="PN977_000618"
     CDS             63767..64657
                     /gene="ilvY"
                     /locus_tag="PN977_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator IlvY"
                     /protein_id="HDI6040687.1"
                     /translation="MDLRDLKTFLHLAESRHFGRSARAMHVSPSTLSRQIQRLEEDLG
                     QPLFVRDNRTVTLTEAGEELRVFAQQTLLQYQQLRHTIDQQGPSLSGELHIFCSVTAA
                     YSHLPPILDRFRAEHPSVEIKLTTGDAADAMEKVVTGEADLAIAGKPETLPGAVAFSM
                     LENLAVVLIAPALPCPVRNQVSVEKPDWSTVPFIMADQGPVRRRIELWFRRNKISNPM
                     IYATVGGHEAMVSMVALGCGVALLPEVVLDNSPEPVRNRVMILERSDEKTPFELGVCV
                     QKKRLHEPLIEAFWKILPNH"
     gene            complement(64654..66198)
                     /gene="ilvA"
                     /locus_tag="PN977_000619"
     CDS             complement(64654..66198)
                     /gene="ilvA"
                     /locus_tag="PN977_000619"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase, biosynthetic"
                     /protein_id="HDI6040688.1"
                     /translation="MADSQPLSGAPEGAEYLRAVLRAPVYEAAQVTPLQKMEKLSSRL
                     DNVILVKREDRQPVHSFKLRGAYAMMAGLTEEQKAHGVITASAGNHAQGVAFSSARLG
                     VKALIVMPTATADIKVDAVRGFGGEVLLHGANFDEAKAKAIELSQQQGFTWVPPFDHP
                     MVIAGQGTLALELLQQDAHLDRVFVPVGGGGLAAGVAVLIKQLMPQIKVIAVEAEDSA
                     CLKAALNAGHPVDLPRVGLFAEGVAVKRIGDETFRLCQEYLDDIITVDSDAICAAMKD
                     LFEDVRAVAEPSGALALAGMKKYIAQHNIRGERLAHILSGANVNFHGLRYVSERCELG
                     EQREALLAVTIPEEKGSFLKFCQLLGGRSVTEFNYRFADAKNACIFVGVRLSRGLEER
                     KEILQMLNDGGYSVVDLSDDEMAKLHVRYMVGGRPSHPLQERLYSFEFPESPGALLRF
                     LNTLGTHWNISLFHYRSHGTDYGRVLAAFELGDHEPDFETRLNELGYDCHDETNNPAF
                     RFFLAG"
     gene            complement(66201..68051)
                     /gene="ilvD"
                     /locus_tag="PN977_000620"
     CDS             complement(66201..68051)
                     /gene="ilvD"
                     /locus_tag="PN977_000620"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HDI6040689.1"
                     /translation="MPKYRSATTTHGRNMAGARALWRATGMTDADFGKPIIAVVNSFT
                     QFVPGHVHLRDLGKLVAEQIEAAGGVAKEFNTIAVDDGIAMGHGGMLYSLPSRELIAD
                     SVEYMVNAHCADAMVCISNCDKITPGMLMASLRLNIPVIFVSGGPMEAGKTKLSDQII
                     KLDLVDAMIQGADPKVSDSQSDQVERSACPTCGSCSGMFTANSMNCLTEALGLSQPGN
                     GSLLATHADRKQLFLNAGKRIVELTKRYYEQNDESALPRNIASKAAFENAMTLDIAMG
                     GSTNTVLHLLAAAQEAEIDFTMSDIDKLSRKVPQLCKVAPSTQKYHMEDVHRAGGVIG
                     ILGELDRAGLLNRDVKNVLGLTLPQTLEQYDVMLTQDDSVKNMFRAGPAGIRTTQAFS
                     QDCRWDSLDDDRANGCIRSLEHAYSKEGGLAVLYGNFAENGCIVKTAGVDDSILKFTG
                     PAKVYESQDDAVEAILGGKVVAGDVVVIRYEGPKGGPGMQEMLYPTSFLKSMGLGKAC
                     ALITDGRFSGGTSGLSIGHVSPEAASGGSIGLIEDGDLIAIDIPNRGIQLQVSDAELA
                     ARREAQEARGDKAWTPKNRERQVSFALRAYASLATSADKGAVRDKSKLGG"
     gene            complement(68116..69045)
                     /gene="ilvE"
                     /locus_tag="PN977_000621"
     CDS             complement(68116..69045)
                     /gene="ilvE"
                     /locus_tag="PN977_000621"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain-amino-acid transaminase"
                     /protein_id="HDI6040690.1"
                     /translation="MTTKKADYIWFNGEMVRWEDAKVHVMSHALHYGTSVFEGIRCYD
                     SHKGPVVFRHREHMQRLHDSAKIYRFPVSQSIDELMEACRDVIRKNNLTSAYIRPLIF
                     VGDVGMGVNPPAGYSTDVIIAAFPWGAYLGAEALEQGIDAMVSSWNRAAPNTIPTAAK
                     AGGNYLSSLLVGSEARRHGYQEGIALDVNGYISEGAGENLFEVKDGVLFTPPFTSSAL
                     PGITRDAIIKLAKELGIEVREQVLSRESLYLADEVFMSGTAAEITPVRSVDGIQVGEG
                     RCGPVTKRIQQAFFGLFTGETEDKWGWLDQVNQ"
     gene            complement(69065..69328)
                     /gene="ilvM"
                     /locus_tag="PN977_000622"
     CDS             complement(69065..69328)
                     /gene="ilvM"
                     /locus_tag="PN977_000622"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 small subunit"
                     /protein_id="HDI6040691.1"
                     /translation="MMQHQVNVSARFNPETLERVLRVVRHRGFHVCSMNMAAASDAQN
                     INIELTVASPRSVDLLFSQLNKLVDVAHVAICQSTTTSQQIRA"
     gene            complement(69325..70971)
                     /locus_tag="PN977_000623"
     CDS             complement(69325..70971)
                     /locus_tag="PN977_000623"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001012636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 catalytic subunit"
                     /protein_id="HDI6040692.1"
                     /translation="MNGAQWVVHALRAQGVNTVFGYPGGAIMPVYDALYDGGMEHLLC
                     RHEQGAAMAAIGYARATGKTGVCIATSGPGATNLITGLADALLDSIPVVAITGQVSAP
                     FIGTDAFQEVDILGLSLACTKHSFLVQSLEELPRIMAEAFDVASSGRPGPVLVDIPKD
                     IQLASGDLEPWFTTVENEVTFPHAEVEQARQMLAKAQKPMLYVGGGVGMAQAVPALRE
                     FLATTKMPATCTLKGLGAVEADYPYYLGMLGMHGTKAANFAVQECDLLIAVGARFDDR
                     VTGKLNTFAPHASVIHMDIDPAEMNKLRQAHAALQGDLNALLPALQQPLNIDDWQQHC
                     AQLRDEHAWRYDHPGDAIYAPLLLKQLSDRKPADCIVTTDVGQHQMWSAQHIVHTRPE
                     NFITSSGLGTMGFGLPAAVGAQVARPNDTVVCISGDGSFMMNVQELGTVKRKQLPLKI
                     VLLDNQRLGMVRQWQQLFFQERYSETTLTDNPDFLMLASAFGIPGQHITRKDQVEAAL
                     DTMLNSDGPYLLHVSIDELENVWPLVPPGASNSEMLEKLS"
     gene            complement(70974..71024)
                     /locus_tag="PN977_000624"
     CDS             complement(70974..71024)
                     /locus_tag="PN977_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6040693.1"
                     /translation="MNNSTKFCFSRFRTGN"
     gene            complement(71111..71209)
                     /gene="ilvL"
                     /locus_tag="PN977_000625"
     CDS             complement(71111..71209)
                     /gene="ilvL"
                     /locus_tag="PN977_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ilv operon leader peptide"
                     /protein_id="HDI6040694.1"
                     /translation="MTALLRVISLVVISVVVIIIPPCGAALGRGKA"
     gene            71562..73082
                     /locus_tag="PN977_000626"
     CDS             71562..73082
                     /locus_tag="PN977_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312727.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HDI6040695.1"
                     /translation="MSLSIVHTRAALGVNAPPITVEVHISKGLPGLTMVGLPETTVKE
                     ARDRVRSAIINSGYEYPAKKITINLAPADLPKEGGRYDLPIAIALLAASEQLTANKLD
                     EYELVGELALTGALRGVPGAISSATEAIKSGRKIIVAKDNEDEVGLINGEGCLIADHL
                     QAVCAFLEGKHALERPKPTDAVSRALQHDLSDVVGQEQGKRGLEITAAGGHNLLLIGP
                     PGTGKTMLASRINGLLPDLSNEEALESAAILSLVNAESVQKQWRQRPFRSPHHSASLT
                     AMVGGGAIPGPGEISLAHNGVLFLDELPEFERRTLDALREPIESGQIHLSRTRAKITY
                     PARFQLVAAMNPSPTGHYQGNHNRCTPEQTLRYLNRLSGPFLDRFDLSLEIPLPPPGI
                     LSKTVVQGENSTTVKQRVMAARERQFKRQNKLNAWLDNPEIRQFCKLESEDAQWLEET
                     LIHLGLSIRAWQRLLKVARTIADIDQSDIITRQHLQEAVSYRAIDRLLIHLQKLLT"
     gene            complement(73107..73445)
                     /gene="maoP"
                     /locus_tag="PN977_000627"
     CDS             complement(73107..73445)
                     /gene="maoP"
                     /locus_tag="PN977_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ori organization protein MaoP"
                     /protein_id="HDI6040696.1"
                     /translation="MAESFTTTNRYFDNKHYPRGFSRHGDFTIKEAQLLERHGYAFNE
                     LDLGKREPVTEEEKLFVAVCRGEREPVTEAERVWSKYMTRIKRPKRFHTLSGGKPQVE
                     GAEDYTDSDD"
     gene            73564..74403
                     /gene="hdfR"
                     /locus_tag="PN977_000628"
     CDS             73564..74403
                     /gene="hdfR"
                     /locus_tag="PN977_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator HdfR"
                     /protein_id="HDI6040697.1"
                     /translation="MDTELLKTFLEVSRTRHFGRAAESLYLTQSAVSFRIRQLENQLG
                     VNLFTRHRNNIRLTAAGEKLLPYAETLMSTWQAARREVAHTSRHNEFSIGASASLWEC
                     MLNQWLGRLYQNQDAHTGLQFEARIAQRQSLVKQLHERQLDLLITTEAPKMDEFSSQL
                     LGYFTLALYTSAPSKLKGDLNYLRLEWGPDFQQHEAGLIGADEVPILTTSSAELAQQQ
                     IAMLNGCTWLPVSWARKKGGLHTVVDSTTLSRPLYAIWLQNSDKNALIRDLLKINVLD
                     EVY"
     gene            complement(74498..74573)
                     /locus_tag="PN977_000629"
     tRNA            complement(74498..74573)
                     /locus_tag="PN977_000629"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(74538..74540),aa:Trp,seq:cca)
     gene            complement(74582..74658)
                     /locus_tag="PN977_000630"
     tRNA            complement(74582..74658)
                     /locus_tag="PN977_000630"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(74622..74624),aa:Asp,seq:gtc)
BASE COUNT        18314 a        19970 c        18913 g        17472 t
ORIGIN      
        1 tttgcactct ggcccgccga ttgcctggga agagatgtgt ggtccggtaa aaggggcgat
       61 tattggtgcc atgctgtacg aaggttgggc caccagccag caagacgctg aaaaccaaat
      121 aaacgcaggc gaaatcgacc ttgccccttg tcatcattat cacgcggttg gcccaatggc
      181 aggcatcatc agcccgtcga tgccgttatg ggtggtagaa aacaaaacca acggccaccg
      241 tacttttagc aatttcaatg aaggtctggg taaggttctc cgttttggcg ccaataacga
      301 tgaagttctc aatcgtctgg cctggatgcg cgatgaactg gcgccggcaa tgaaagcagc
      361 aatcgcacaa cacggcgagc tggaactcaa gccattaatg gcgcaggcgc tgcatatggg
      421 ggatgaagtt cataaccgca atgctgccgc taccggtttg ctgattaagc gcctgctccc
      481 ggcattgctg gcctgctctc ttccacagga acatatccag cgcgtcgtgg catttatcac
      541 cggtaacgat cacttcttcc tcaatctttc gatggccgcc tgtaaagcaa tgatggatgc
      601 cgccgccaat gtaccgttca gttcaatggt tacggtgatg gcgcgtaacg gggtgaattt
      661 tggtattcgg ctttccggta ctggcgatcg ctggttccag gctcctgcca atgccgttga
      721 aggtctgttc ttccccggat ttggcgtcga cgatgccgct gctgacctgg gtgatagcgc
      781 aattactgaa accgcaggtg tgggtggctt cgcgatggcg tcgtctccgg cgatcgtcaa
      841 atttgtggga ggcacccctg ccgatgccac caataacagc cgtcggatgc aagccgtcac
      901 cctcggcggt aaccccgcat ttacacttcc agccctcaat tttgccccca ccgcagcagg
      961 gattgatgcg cgtaaagtga ccgatcgcgg cattttaccg gtcatcaata cgggtatcgc
     1021 ccacaaacaa gcgggggtcg ggcaaattgg tgccgggatc accactgcgc cgatggcctg
     1081 ttttgtcgcc gctgtacgtg cactggcgga gatcgtcgca aaggagaacc atcatggtta
     1141 agccactggc tgtcgtcgcg gttggcggca atgcgctcat tcaggacgag caacgcaata
     1201 gtattcccga tcaatatgtt gcagtgatgg aaagcgtgca gcatatcgtt gatatggttg
     1261 aagccggatg ggacctggtg ctaacccacg gtaatggccc gcaggtgggc tttattctgc
     1321 gccgctctga actcgccagt aacgaagttt ctccggttcc acttgattac gccgtgggtg
     1381 atacacaagg tgcaattggc tacatgttcc agaaagcact gcataacgaa ttggctcgcc
     1441 gtggcataaa caaaccggta attgccctgg tgacacaaac gcgagtcagc ccacatgacg
     1501 atgctttcgc cagccccagt aaaccaattg gcgcgtttct cgatgaagca acggcccaac
     1561 aacgccaaca acaactcggc tggacgctga tggaggacgc cgggcgtggt tggcggcgta
     1621 cagttccctc tcctgcccca ctggaagtta ttgagcacga caccatcgct cacctggtgc
     1681 gccagggata tctggttatt gcctgcggcg gcggcggtat tccggtggtg cgagacggac
     1741 aacaactgaa aggtgtagaa gccgtgatcg ataaagatct ggcctccgcg ctgctcgcca
     1801 gtcagttagg cgcagatctg ctggtgatcc ccaccggtgt agaaaaagtg gcgattaact
     1861 ttggtacacc acaacaacag tggctcgacg ctatcagcgt tgccgaagcg caaacgctgt
     1921 tgcgggaagg tcagtttggt gtcggcagta tgcaacccaa agtggaagcc attgttgatt
     1981 tcatcaatgc cagccagcaa caaggcaaac aggccagcgg cctgattact tcaccgcaaa
     2041 ccataaaagc agccctggcg catcagagcg gtacatggat aaccctttaa ttctctggag
     2101 aacacaatga ctacaacgct tttagccgtt aacggaacgc tgatgcgcgg cctggaactc
     2161 aacccaaata tgcaaaaagc gggcggtatt tttgtccgtg aagaccgaac tgacgcccat
     2221 taccggttgt ggagtattaa tgatcgccat ccgggaatga ttcgcgtgaa tgaaggagga
     2281 acacacgtcg acgtagaaat ttggcaatta ccgctggcca gttttgccgc tctgttaatg
     2341 agcgaacccg ctgggctggc aattggcaaa atcaaacttg ccgatggtag cgaagtgctg
     2401 ggcgtactgg cagaaaactg gctgaccgaa ggccaacggg aaattacaga actgggtagc
     2461 tggcgcaaat acaccgggca ttttcatact gtgtaacgcg catttataaa gggaacatac
     2521 tatgcgtaaa aaagaagaga atctgaatac ggcatcagga ttgcgtattg ccatgatttt
     2581 gctgggtatt gccgtcacac ctgtgctgtt gtcatcctca agcctcggca atcaactttc
     2641 cagcggcagt ttaattagcg tcgtattgtt aggcggcgtc attctgacct tactttcagc
     2701 catcaccatt agcgtgggag aaaaagcccg cctaccaacg tatggcattg tgaaatattc
     2761 gtttggcgaa aaaggggcca tcgccattaa cattttgatg gcgataagtc tgttcggctg
     2821 gattgccgtt accgccaata tgtttggtca ttcggtacat gacttactgg ctcaacatgg
     2881 actggaagtt ccactggcac tgttagtggc ggctggctgt gtcatttttg tcgcctctac
     2941 ggcatttggc tttgccgttc tgggaaaaat tgcccaggtt gccgtgccgg ttatcgcgct
     3001 ggtgctgtgt tacatcctct atgtggcaac ccataccgaa gtggcagtac cagcggcgat
     3061 tgtggagatg aatacaggtg tcgccgtttc caccgttgtt ggcaccatta ttgtgctggt
     3121 tgccacactg cctgatttcg gtagttttgt gcataaccgc aaacatgcgc tgattgccgc
     3181 aggcgtgacg tttctggttg cctaccctct gctctactgg gcgggtgcaa cgccgagcgc
     3241 cattagtggt cagggatctt tactgggtgc gatggcggta ttcggtgcgg ttctgcctgc
     3301 ggcgctgttg ttgattttcg cctgcgtcac cggtaacgcg ggcaatatgt tccagggcac
     3361 gctggtggtt tccacactgc ttacccgctt tcccaaatgg cagattaccg tggcgctggg
     3421 tatcctttcc gccatcgtag gcagtatgga tattatggcg tggtttattc cgtttctgct
     3481 gttcctgggt atcgccacgc cacccgttgc cggaatttat atcgctgact ttttctttta
     3541 tcgccgtaat ggctatcaag agtcagtgtt agcccaggag tcacagatta aagtgctgac
     3601 attcgcagca tggatcatag gcgcagcggt tggctttatg accgtaaaag gcttattcac
     3661 cctgacgacg atcccttcgg tagactcgat tctggtggca tgtatcgctt atgcgattct
     3721 cagtcgggca agtcaacacc gctaattcct gggtatactc aggcaatgat tacggctgaa
     3781 tctggagaga ttcagccact ttaccactga ccaggaagcc ttatgttcat ctcagacgaa
     3841 acgcttcgtg ttattcatct ggtcgccaga caccagagca ttaccaccgc agccgagcag
     3901 ttaaataaag tcccttccgc catcagttac accatcaaga aagcggaaga gagcctgggc
     3961 gttgaactgt tcattcgtaa gggacgctat attgaattat cgccagcggg aagttacttc
     4021 atcgaacata gcaaaactat tcttggcgac ctggaagcat taaagcgcaa cacagtgctg
     4081 attcacgatg gtattgagcg tgaactgaca attgcggtaa acaatattat tcctggtgat
     4141 ttgctggtgg catttattcg agattttgaa cggcaattca catcaacaac actgacggta
     4201 gatcttgagg tatacaacgg ctgctgggat gcgctgtaca gtaagcgtgc cgatctggtc
     4261 tacggtgctc ctcatgcagt gccaagcagt gaaggcatca ttagcgaacc ggtcggacaa
     4321 atggaatggg attttgtcgt cagcccactc cacccactgg cagcgaagcg gcatccatta
     4381 gaaaacagtg aattgcgtca ttatccagcg gtttgtatcc gcgatacctc cgttaacttc
     4441 cccccgatgc aagcctggtt gttggaagga caaaaaccca tttttgttcc tgacttcgct
     4501 acggcaatcg ccttaataga acaaaacgtc ggtattggtt atattccaca tcatctggca
     4561 ttgccgttat tgaacagcgg taaattgctt aaaaagccaa tgcgcgaaca taaacacgcc
     4621 accaagttat ttcttgccgc tcgttccgat ggtatgggca aagcctgtca gtggtgcata
     4681 gaatatttaa gaaatccgca attaattttc tgttcctgaa gatccacatc atacctggca
     4741 tacatagcaa aatggcttcc ggtgttggaa gccattattg tgctgcaatt tacaatgact
     4801 cgttaatact catcatcccc gacgcaaatt ctgcgccgcc tgcaccatgt tcgccagtgc
     4861 cgcgcgggtt tctggccagc cgcgcgtttt caggccacag tccgggttga cccacagacg
     4921 ctctgccgga atgcgttttg ccgctttctt cagcaaggct tcaatccatt ccacgctcgg
     4981 tacgtttggc gagtgaatgt catagacgcc aggaccgatt tcatttggat aatcaaactc
     5041 ttcgaacgac tccagcaact ccatgtcgga acgcgaggtt tcgatggtga tgacgtctgc
     5101 gtccagcgcc gcaatcgaat ccatgatgtc gttgaactcg cagtaacaca tgtgagtgtg
     5161 gatttgtgtg tcatctttcg ccacagcggc gttgatacgg aaagcctcca cgccccactg
     5221 gagataggca tcccagtcgc tgcgacgcag tggtaaacct tcgcgcaatg ccggttcgtc
     5281 aatctgaatg atgccaattc cagcggcttc caggtccgcg acttcatcac gcagcgccag
     5341 cgcaatttgt ttggcgatgg tttcacggct gacatcttca cgcgggaacg accagcagag
     5401 aatagtcacc gggccggtca acatcccttt caccggttta tcagtcaggg attgcgcata
     5461 ttttgcccac tccacggtaa tcggtgccgg gcggctaacg tcaccaataa caatcggtgg
     5521 cttcacgcag cgggaaccgt agctctgtac ccaaccgttt tgcgtaaaga caaagccatc
     5581 cagatgctcg ccaaagtatt ccaccatgtc attacgctcg gcctcgccat gtaccagcac
     5641 atccagtccc aaacgttcct gctcaacaat ggcctgcttg atatgttccg caatgcctgt
     5701 gcggtagttg ttggcgtcga gattaccctt tttgaaatcc agacgcaggg tacgaatctc
     5761 cgtggtttgc gggaaagaac caatcgtggt ggtcggccat gctgggagtt taaaacgcgc
     5821 acgctgggct tcagcacgca cttcatagac attcgcacgc tgactgtcct gagcggtgat
     5881 cgccgccaga cgcttttcta ctgccggatt atgtacacga gtagagtgac gacgcgcctg
     5941 aatcggagcg ctccacgctg ccagagctgc cgtatcacca ctgttcaacg catcgcgcag
     6001 caatgccagt tcatgacatt tttgcagggc gaaggcaaac cagcttttca cttctgcatc
     6061 aagacgtgtt tccacactca ggtcaatggg actgtgcagc agtgagcagg aagatgccac
     6121 ccacaaatca cgtttgccga caatgtcctt aatttgcgca tatttctcgg taagatcggc
     6181 gcgccagacg ttacgaccat tgataagacc cgcagacagc agccagtcag aaggcagacg
     6241 cttgtgcagt tcagcaacgt catctttacc atgtacaaga tcgacatgca gaccctgaac
     6301 aggcagcgca gtaatcgtgt cgagatttgg tgttacgcct tcaaaatagg tggtcagcag
     6361 cagtttcacc tgtccctgga gcgcgtcgta agcgggtttg tatgcgtcca gccacgcctg
     6421 cggcagttcc agtaccagcg cgggttcatc aatctgtacc cactcgatgc cgcgtttcgc
     6481 cagttctgcc agcacttgct gataaaccgg cagaatgtcg ttcagcaggc tcaggcgatc
     6541 aaactgttca cctttcactt tacccagcca caggtaggta actggcccca gcagcacagg
     6601 tttcaccttg tggcccagcg ccagcgcctc gtccacttcc tccagcagct gcgtccaggt
     6661 cagtttgaac tgttggcctt taacgaactc cggcaccatg tagtgatagt tggtgttaaa
     6721 ccatttggtc atttccgctg ccgccgcagg ttcgccagtc ggtgcacgtc cacgaccaat
     6781 acggaacagg gtgtcgatat ctaccgaacc atcgttgttc tggtgacgcg ccggaacatt
     6841 acccagcagc agactggtgg tcagtacatg atcgtaccag gcaaaatcgc ccaccggcag
     6901 caggtcgata cccgcttgct tttgttgctc ccagtgacga gcacgtaatt cacgccctac
     6961 cgccagcagt tcttcacgcg tggagttccc cgcccaataa ctctcttgcg cttttttcag
     7021 ctcgcgacgc aggccaacgc gagggaaacc gagggtgtga ttaagaattg tcattatatg
     7081 ccccttatgg aatttttagt atttagccgt ccagatgttt acacatccat aattggcggt
     7141 tactgtatat tcctcaagcg caaattattc atgccgaagt gaaggacttt catgatcgaa
     7201 gtaaaacacc tgaaaacgct acaagcgttg cggaactgcg gctcactcgc agccgctgcg
     7261 gcgacgttgc atcaaacgca atccgccctg tctcaccagt ttagcgatct ggaacaacgc
     7321 cttggcttcc ggctatttgt gcgtaaaagc cagccgcttc gctttacacc gcagggagaa
     7381 atcctgttgc aactggcaaa tcaggtgctg ccgcaaatta gccaggcact gcaagcctgc
     7441 aatgaaccgc agcagacgcg cctgcgcatt gccatagagt gccatagctg tattcagtgg
     7501 ctgacgcccg cattagaaaa tttccataag aactggccgc aggtggagat ggattttaaa
     7561 tcgggcgtaa cgtttgatcc gcagcctgcg ttgcaacagg gcgagctgga tctggtgatg
     7621 acgtccgata ttctgccgcg cagtggcctg cattattcgc cgatgttcga ctatgaagtg
     7681 cgtctggtgt tagcacctga ccatccactg gcggcaaaaa cgcgaattac accggaagat
     7741 ctcgccagcg agacgctgtt gatttatccg gtgcagcgta gccgactgga tgtctggcgg
     7801 cattttcttc agccagcagg cgtcagcccg tcactgaaaa gcgtcgataa caccttattg
     7861 ttgattcaga tggttgccgc acggatgggt attgccgcac taccgcattg ggtagtggag
     7921 agttttgaac gccagggtct ggtggtcacc aaaaccctgg gcgaaggctt gtggagccga
     7981 ctgtacgccg ccgtgcgcga tggcgagcag cgtcagccga ttacagaagc gtttattcgt
     8041 tcagcgcgca atcacgcctg cgatcatctg ccgtttgtga agagcgcgga gcgacccact
     8101 tacgatgcac ccacagtgag gccaggatca ccagcgcgcc tgtaataaac gttggccagt
     8161 gcggctgttg gtgccagata gccaggttta ccagcagccc tgccggaacg tgcatattat
     8221 tcataatgcc cagcgttccg gcgtccacct gcgtcgcgcc gtagttccac ataaagtagc
     8281 caatcccgga agccaccacg ccaagaaaca ccagaatgcc ccattgcaac gtggtttgcg
     8341 gcattttctg cgcatttcct agcaagaacc atgcaatcac tgccaccagg aacgcgccaa
     8401 gatagaacca cgcaaaggcg ttatgctgcg gcatcgggcg agtttccatc agacgtttgt
     8461 aacccaccat gccgatggca aaagtgatat tggagagttg caccagcaat aagccagtcc
     8521 agaaatggtc ggtgacctga tcatagcgaa taatcccggc accaatcacc gccagcaagg
     8581 cgctaaaagc atagccccag cgcagacggc gcttactcat gatgtcataa atcagcgtga
     8641 tgtagagcgg cgtcagtacg gtgaacagca acagttcgga gaccgtcagg tagagatagg
     8701 cgcggaaact cagcatatac atcacaccga gctgcatcgc gcccaccagc atatacaggc
     8761 cgaccgtttt caggctattg ccacgggtac gcagaaacgg cagaaaaacg agtgccgcca
     8821 ggccaacgcg caccagcacc gcaaaatagc tatcgacgtg ccccgcaagg tactcgccat
     8881 aaaagctaaa ggagaaggcc cacagaatcg tggtgatgat aagtagcgcc acaatggaaa
     8941 actctttgat taacgagtat ccattgtagc gaagtatcag gttgacaact gaccaaataa
     9001 agaacgatta cgataaatag agcttacgca gataatgcgg cactgcgtcc tcggcgttag
     9061 taccaatcac ttccagttcc ggatgcagat ctttcagacg ctggtgcgca ctgcccataa
     9121 tgcagccttt ccccgccatc gacagcattt cggcgtcgtt catgccatcg ccaaaggcaa
     9181 tacaatcttg caggctgtag cccagtttct tcgccaccgc ttccagcgca tggcctttcg
     9241 acacgccgcc cgccatcact tccagacagg ttaaggtaga gaaactaacg ttgacgcgat
     9301 cgccccagcg agcattaatc gcctgctcca gcggcagcag ttgttcatgg gaatcgcagg
     9361 tgaagaacac tttgctgacg ccttccggct ccagtaatcc cggctcatac agcgcatatt
     9421 tgaataccgc ttctttaaaa aagcgcatct cttccgggcg atgacgattc ataaaccatt
     9481 cgtcgtcgcg ataaacgtta gtaatgatgt ccggattgtc gttgactacg ccaaacagat
     9541 cgctggcaat atcgcgatcc aggttatgag caaaaatcag attgccatcc agatcgtgaa
     9601 cgcgcgcgcc attggaggta atcatgtaag acttaatctc cagattatcg cgaatttgcc
     9661 ccacatcaac gtggtgacga ccggtcgcaa atacaaagtt gatgccgcgc gcggtgagca
     9721 gcttcagagt ttctttggcg taaggggata acgtatggtc gggagaaagt aacgtgccat
     9781 ctaaatcaga cgcaacaacc tggtacataa gaaatttaaa cctctgtaga gcggtttccg
     9841 ctgggtgagt tatgcctgtt gaaaaaatca acgatagcgt ggagcgcgac tgagcgcatt
     9901 gcgtcctttt caaaaaggat ctcatggtac gcacctttaa ttaccaacgg ccgtcctcct
     9961 tcgacaggat ggcccgcagc ggtgcgtagt tcacaaaaac ggtcgtgcat gcggttatcc
    10021 accacgcgtt cctcttcagc ttgtaagaga agcgttggcg tggcgtcatc acccgcaccg
    10081 gctaacacct gttcgccagc cagaatactt tcgcgtaccc aatggtaggt cggcccaccg
    10141 acgcgaatcg ttgggtcatc agcataaaag cgtaagttac gtcgatatcg ctgtctgctg
    10201 tgggtcagta cgttgatagc aaacggcaac gcgcgccagc gcccggtgcc tattgcatag
    10261 ccatcacgga aacgtggatg cgcttcggcc cagttgagga tctgccgtgc cataaatgac
    10321 ggcatacgaa tcacgatccc aaacattggc gcagttagcg caatggcgtc acatacacct
    10381 ggatggcgtt gcagaaataa tgtggagatc gcaccgccca ttgaatgtgc cagtatatag
    10441 cgtttacgcc acggaccggg ctgaacctcc tgctgccaga atgccgccag atcatcaaca
    10501 taatcattaa agcgattaac atgcccgaga tgcggatcgg ctaacaggcg accggaacgt
    10561 ccctgcccgc gatggtcgat gattaagaca tcaaacccca aatggaacag gtcataggcc
    10621 agttccgcat attttacgta gctctcaata cgccccgggc agatgactac cacccggtca
    10681 tggtgctgtg cgcgaaaacg gacaaagcgc accggaatgt catccacacc agtaaattct
    10741 gcttcatcac gctgacgcca gaaatcagtc agcggtccca tggtaaaagc agcaaacgcg
    10801 ttttctcttg tttcccagtc tttttgctgc tgaaacatcg ggtaatctgc ctcttaaacc
    10861 acgtaaaatc gtttttttaa gcgtgcctga cacaacgctg cgacagtagc gtattgtggc
    10921 acaaaaatag acacaccggg agttcatcat gaccttagaa tggtggtttg cctacctgct
    10981 gacatcgatc attttaagcc tgtcgccagg ctctggcgca atcaacacca tgaccacctc
    11041 gctcaaccac ggttatcgcg gcgcggtggc gtctattgct gggcttcaga ccggactggc
    11101 gattcatatt gtgctggttg gcgtggggtt ggggacgcta ttttcccgct cagtgattgc
    11161 gtttgaagtg ttgaagtggg caggcgcagc ttacttgatt tggctgggaa tacagcaatg
    11221 gcgcgccgct ggtgcaattg accttaaatc gccagcctct actcaatcgc gccgacattt
    11281 gttccagcgc gcagtttttg tgaatcttac caatcccaaa agtattgtgt ttctggcggc
    11341 gctatttccg caattcatca tgccgcagca gccacaactg atgcagtata tcgtgctcgg
    11401 cgtcaccact attgtggtcg atattattgt gatgatcggt tacgccaccc ttgctcaacg
    11461 gattgctcta tggattaaag gaccaaagca gatgaaggcg ctgaataaga ttttcggctc
    11521 gttgtttatg ctggtgggag cgctgttagc atcggcgagg catgcgtgaa aaataatgtc
    11581 ggatgcggcg taaacgcctt atccgactta ctctgatgac gcgtctggca tcaacgcgaa
    11641 ataatcaaat gaatgccaaa tccggcaaat aacgccccgg caaaaccatc aatccacttc
    11701 gccagacgtt gataaccacg gcgcatttgc ggcagggcaa acaggctggc aacgacggta
    11761 aaccacgcca gtgtttcgat aataatcaac gcgaaaatgc cccagcgctc agtggtgccg
    11821 acgttatcgc cgacaaacag cgagaatacc gaaccaaagt agataatcgc tttcggattg
    11881 gcgagattgg tcagtaagcc tttcaggaaa ctgcgcccgc tttttgccag ctcaacctgt
    11941 ggtgcaggcg cagaaaccgc ctcttttttc agtgcaccgc gtagcatctg gtaacccatc
    12001 cagcagagat acaggccacc gcccaccata atcagcgtat gcagccaggc cattttttcg
    12061 ataatcaaat gtaggccaag caacgcaatc ccagcccaaa ccattacgcc gcaggtaatg
    12121 cccagcacgc ccatcatcgc ttctttacgg gaacgactga cagcggtctg agagacaaaa
    12181 aagaaatcgg gaccggggct cataagcgcc acaatgtgca ccatggcgac ggtgaggaat
    12241 aacatcaaca tacattagac tcgcggggga aacgtttacg gagtcttcat cctggcactt
    12301 ttttatgctg ctgactactc ttcgtcgtca ccatcgacat gcgcgcggat aagcgccata
    12361 aacggtttac caaagcgttc cagcttgcgc atcccaacgc cgttaacgct gagcatttcg
    12421 ctggcggtga tcggcatctg ttcagccatc tcaatcaagg ttgcgtcgtt aaacaccacg
    12481 tacggcggga cattgctttc atcggcaatc gatttacgca gtttgcgtaa tttggcgaac
    12541 agtttgcgat catagttgcc gccgaaggat ttctgcatcg ctttcggttt gagcgccacg
    12601 atacgcggca cggcaagttg caaagaggat tcgccgcgca gcaccggacg cgcagcctct
    12661 gtcagttgta gggcagaatg ctgggcaata ttttgcgtca ccaggccgag gtgaatcagc
    12721 tggcggatca cgctcaccca atgttcatgg cttttatcac ggcccatgcc atagactttc
    12781 agtttgtcat gaccatagtc gcggatacgc tgattattag caccgcgaat aacttccacc
    12841 acatagccca tcccaaaccg ctgattcaca cgaccaatgg tggaaagggc aatctgagca
    12901 tcggttgaac catcgtattg tttcggcgga tcgaggcaga tatcgcagtt cccgcacggc
    12961 tcctgccgcc cttcgccaaa gtagttcagc agcaccagac gacggcaagt ttgcgcttcg
    13021 gcaaacgcgc ccatcgcatt gagtttatgg cgctcgatat cctgcaattg cccctgcggc
    13081 ttctcttcca gacaacggcg tagccacgcc atatcggccg gatcgtaaaa cagcatcgct
    13141 tccgcgggca ggccatcacg cccggcgcga ccggtttcct gataatagga ttcgatattg
    13201 cgcggaatat caaagtggac cacgaagcgg acgttaggct tgttgatgcc catgccaaac
    13261 gccacggtcg ccaccacaat ttgcaggtca tcgcgctgaa atttttcctg cacgtcggcg
    13321 cggacattat tttccagccc ggcatgatag gccgccgcgc taataccctt gctttgcagg
    13381 cgcgcagcgg tgtcttctac tttcgcccgg ctgttgcagt agataatgcc ggacttaccg
    13441 cgctgttcct gcacgtagcg catcaactga tcgagcggtt tgaacttctc catcagcatg
    13501 tagcgaatat tcggacgatc aaaactgctg atttgaatca gcggatcgtt cagccccagc
    13561 aggcgcacga tatcctggcg cgtggtgtca tcggctgtgg cagtcagcgc cataaacggc
    13621 agcgtcggaa aacgctgacg cagctgaccg agggcagcgt attccgggcg gaaatcgtga
    13681 ccccattggg agatacagtg cgcttcatca acggctaata acaccggatt ccagtgcgcc
    13741 agatgctcaa gaaagttatc cagcatcagg cgttccggcg cgatataaag caggcggatt
    13801 tgcccggtgc gacagcctgt catcacttca agttgctgct cgcgggtttg cgtcgagtta
    13861 aggcacaccg ccgccacgcc gttggcttgc agttgatcca cctgatcttt catcaacgaa
    13921 atcagcggtg aaacaaccac ggtaaggccg tttagcaata aggcagggat ttgatagcaa
    13981 agggattttc cgccaccagt gggcatgaca acgaggcaat cgcggccgga aagcacagtg
    14041 tcgataattt cttcctggcc ggggcgaaac tgttggtagc caaaggtttc ttgtaaaacc
    14101 tgtttagccc cggactccag attcaacact tccgcctgcg ccacattcac cccgattgcc
    14161 tgaaataaaa acaggcgcta ttttcagcgc ctgcgcgaga aactgcaatg cttttagtct
    14221 ctgagagggt aatcctggat gctggattcc agatttgcca accattcatc aaaacaggtc
    14281 gtttagcata actcccacac caacgcgggt ctggttgaag ttatagtcga tgagcgattc
    14341 gccatagccg ctgtaaacct gagtataaag gcgcacatgt ttggtgatcg ggtaacttaa
    14401 gcctaactcc gcgccgccgt agcccgtgtt ccagttgtac tgtcctttcg cactcaacac
    14461 cgcatcaccg aggtgatagc cgattttaag ctggtagtaa cccatatatt tggtgatatc
    14521 cgggttatcg tcagtattac ccaccacata ccacggcttc acctctacca gccagttacc
    14581 gttttctgcc atcaggcgtg tataaaggcg gttccagctg cgggaggtcg ggtctgaacg
    14641 cccgttagag tcgtggttat aacccatctc cacatcgcgc agcgtccaac ctgcaaaacg
    14701 gtaatcggtg gcaaaaccga ggaacaattg cggttcgtag ttggtttcac gaaacggtga
    14761 agactcttcg ctattggaca gttgccacca ggatttttgc gtgtaagacg cacccaatac
    14821 cgagttcggg cctaaaatcc cacgccacag cggaaatgcc aggctcaact gaaactttac
    14881 ttcatcctta cgcgcatttt ccgcccagtc gtaactggca atcgcttctt tattcagatc
    14941 gctggtttgg gtgtagatga tgtagttggt gtcataagga taaagggtga acggattgtc
    15001 atgctcctgc agcatattgg cgataatact gccacgcacc gctggcgcgt catgcacctc
    15061 tttcaccgtt gcctcttgtg catatactgc cataggcaac ataaacaccg gcaacaacca
    15121 gccctgcaga gtccgcatcg ctcgtgttct ccgtaacgaa ttattgataa ggtgaatatt
    15181 tttctgcggg ctattttaca tacttaactc catagccgtt agttatccct tcctaaagta
    15241 gaaatcaaca ataaagaagc ataaaatcaa catttcatta actaatgaag tgtaaggatg
    15301 atatgtctgc cgtactgacc gctgaacaag ccctgaaatt agtgggtgag atgtttgttt
    15361 atcacatgcc gtttaaccgc gcattgggga tggaactgga gcgttacgaa aaagagttcg
    15421 cacagctggc ctttaaaaat cagccaatga tggtgggtaa ctgggcgcaa agcattttgc
    15481 acggcggggt cattgcgtcg gcgctggatg tcgccgccgg tctggtgtgc gtgggaagta
    15541 ccttaacccg ccacgaaacc atcagtgaag atgaactacg ccagcggcta tcgcggatgg
    15601 ggaccattga tcttcgcgtc gattatctgc gcccaggcag aggcgagcgt tttactgcta
    15661 ccagtagcct gttacgtgca ggcaataaag tcgccgtcgc ccgcgttgaa ttacacaatg
    15721 aagaacagct ttatattgcc agtgccaccg ccacctatat ggtaggttga agacataaaa
    15781 gcaggtaaac tgctgttacc aattaaaaat gagtattccc gatggatgca aaacaaacgc
    15841 ggcagggcgt attactcgct cttgccgctt attttatttg gggtattgcg ccagcgtact
    15901 tcaagctgat ttactacgtg cccgccgatg agatcctgac gcatcgcgtg atctggtcgt
    15961 ttttctttat ggtggtgctg atgagcattt gccgccagtg gtcctattta aaaacgctga
    16021 ttcagacgcc acagaaaatt tttatgctgg cagtctctgc cgtgctgatt ggtggcaact
    16081 ggctactgtt tatctgggcg gtgaacaatc accatatgct ggaagcgagc cttggttact
    16141 ttattaaccc actggtgaac attgtgctgg ggatgatttt cctcggcgag cgattccgcc
    16201 ggatgcaatg gctggcggtg attctggcga tatgtggcgt gttggttcag ttgtggactt
    16261 ttggttcact gccgattatc gcgctggggc tggcgtttag ttttgccttc tacggcctgg
    16321 tacgcaagaa gattgctgtt gaagcgcaaa ccggcatgtt aatcgaaact atgtggttgc
    16381 tgcccgtggc ggcgatttac ctgtttgcta ttgccgacag ctcaaccagc catatggggc
    16441 aaaacccgat gtcgctgaat ttactgctga tcgccgccgg tattgtcact accgtaccgc
    16501 tgttgtgttt tactgcggct gcgacacgcc tgcgtctctc aacgttaggc ttttttcagt
    16561 acattggccc gacgctgatg ttcctgctgg ctgtgacgtt ttatggtgaa aaaccgggtg
    16621 ccgataagat ggtaaccttc gcctttattt gggtggcgct ggcaattttt gtgatggatg
    16681 cgatttatac gcagcgtaga acgtccaaat aaaaaataaa tatattctga aaaaatatgc
    16741 cgactcttgc atgagagtgg gcatattgcc atgctgacag gagactcaca taaggaaagg
    16801 cagaatttcc agacgtcgtt gtatattttc atttgcttta ataaatttca tttttggtaa
    16861 attcagcatt atgttattag cgatgagcaa gaccaatcat taaccaagtt ctcttcagaa
    16921 tgtaatgaaa aatgtgaagt gcatttcttt tttaaaattt tttaccctat tatttcttat
    16981 caaattaaca ttgcccttaa ttataagaaa ggagaatata aatgaacaac atattaggct
    17041 catcaattaa tatgccctgt acacttttcg aaactattac tttattcgat gatttttctg
    17101 ctgatgatat gcagtatggg gatatggaag aacaagattt tctctcactc ggcttaagcg
    17161 acatctctgc aaaagttgat ccttaccgcc tcattaaata ccattttcct gggccgaatt
    17221 ctacttatgg tgcttttagt gttccaactt ctggaacaaa aatatcacaa agtgaatgta
    17281 ttgatatttt atttgcagaa atgaaagatc tggcaaaaat gttttccttt tttggacaat
    17341 acaaaacact catccaggat ctgattgatc attttagata tggaaatggc aacagtttcc
    17401 atagccagga attgaactta tcttttcatg aacgaataaa caaatatgat tataatagcc
    17461 caatacgtgt aatcaaagaa tgtatcgaaa atgatatttc ttcaacacct acgataggat
    17521 accggcctct tctcctacaa aagattaaga ccgaattact ttcatcaaga ctaaataaat
    17581 tcaatgattt taaagataat tttaatggca tgggaatcag cattcatgat atctcagctc
    17641 aaaaaatatc tcttttaaat tttcaaaaat atcctatggg ctggagcgct accattcatt
    17701 tcatagcaca agaccacttt ggtttggatg tgactgatat aaaaaacaag atttacaaca
    17761 agtatagatt ttttcgcata tggttctttt tacaacggca taaagacttt gctttcaaac
    17821 cttttttcac caattttaac actatcgaaa gaatcgaaaa ttatctatga aaagaaaact
    17881 ccagaaaatt atattatatt tatttcttct atgctgcatc tataatttat ggacattgcg
    17941 tcctgtacag attttgtata catactctga tgctggcaat tcagtctttc tggtcgttga
    18001 tcatctgcca tggactgaca gcgataaaat aaattggtat ctgaagcatc agaatgaaat
    18061 taaaaatcaa caccccctac cagaaggtag ttggcatacc tggtatgtta ttgatatcgg
    18121 taatggcttc actgactata aaaaatatat cgaagggccg tatgaagatc tctactgttt
    18181 cccgacgatt aagagcaatg ataattgcat cgtaaaaaac tatttaatgg ttattaatga
    18241 atatccttac cgtaatactc atttttttat tgctgataac aatgagtatc aactaaccca
    18301 ggaaaataag atagagcgag tgttcaaccc gcatgacttt gaaaaagaca attttcagca
    18361 ataagccgga taaagaacaa tctttacccg gcatttatat tacaaccagt tcttccgctt
    18421 aaagtacaga tacggtgcca ggcccgcgag gatcataaag ataatcgcgc cagggtagcc
    18481 gaagctccac ttcagttccg gcataaactc aaagttcatc ccgtagctgg aagcgaccag
    18541 cgtcggtggc aggaacacca cggataccac cgagaagatt ttgatgatgc ggttctgctc
    18601 gatattgata aagcccatcg ccgcctgcat caggaagttc accttctgga acagggattc
    18661 gttatgcggc agcagggatt cgatatctcg caggatttca cgcgcctgct ccagttgccc
    18721 acccggtaag cgtgctttac gtaccaggaa gttgagcgcg cgctgggtat ccatcagaca
    18781 caggcgaact ttccagccga tatcttccag ttccgccagc gtggagagcg cctcgtcgta
    18841 ctcatcgccc tgatgccctt ccataattac ccggctcaac tgctccaggt cactgtagat
    18901 attttcaatt tcatccgcca actgttcgat tttggtttcg aacagatcca gcaacagctc
    18961 gtaggcgtta ccgtccacca tcgactggct acgggcacgc atacgataca gacgaaaagc
    19021 gggcagttca cgctcacgca gagtaaacag acgaccatca cgaatggtaa atgccacagt
    19081 ggagttaccg gcgtgatctt ccgcatcttc aaagaagaag aaggagtgaa tatgcaggcc
    19141 gtcatcgtct tcaaagaaac gtgccgatgc ttcgatgtct tccagttccg ggcgggttgc
    19201 caggctctgg ccaagttcag attgtacgcg cagtcgctcg tcgtcgtccg gttcgacaag
    19261 atcaatccat actgcattta caaggggttg tgactcttcg acttccagcc gggtcagtcg
    19321 gttattttcc agttgaaatg cgctcagcat gaccgggact cccaatgctt aaaatatcgg
    19381 acagttcggt gggcacacag aaacaaattg ggtttcagac cattaaacag cctgactcag
    19441 cgcgacggga aaaattgagg tcgctgacaa ccgctaaggc tatcagcaaa aagggatagc
    19501 cttaggagtt gatcctggat gacaggatag tgagccagta tctactgggt gtgtccaagg
    19561 cgaatgtcct cttagagtga tcgtgcgcgc atgttacgcc agcaaaattt tgccgtcaac
    19621 acgcaacaga acaccacaga acaatagttg aacttcgcgc tttttggtac tcgtcaaggt
    19681 cgctaacagg ataaaaaaat cccacatcca ggacctataa tggcgcatcg gctactcatt
    19741 ggtaaaggaa tgatcacctt gaatctcaaa cggatttttc tcaccttcac cttacttccc
    19801 ttgtttgctg ttgccgctga tgattgcgca ctctcagatc cgacgctgac cgtacaggcg
    19861 tataccgtta atcctcaaac agagcgggtg aaaatgtact ggcaaaaagc caatggcgaa
    19921 gcgtggggaa cgttacatgc tctgctggcg gatatgaata gtcagggtca ggtgcagatg
    19981 gcgatgaacg gcggcatcta tgatgaaagc tatgcgccgc tcggtttgta catcgaaaac
    20041 ggtcagcaga aggtggcgtt aaatctcgct tcaggtgaag ggaatttctt tattcgtcct
    20101 ggcggcgtgt tttatgtcgc gggagataaa gtcggcatcg ttcgtctgga tgcctttaaa
    20161 accagtaaag agattcagtt tgcagtgcag tcagggccaa tgttattaga aaacggcgta
    20221 attaatccac gcattcaccc gaatgttgcc tcgcgtaaaa ttcgtaacgg tgtagggatt
    20281 aataaacaag ggaacgccgt gtttttgttg agccagcagg cgacaaattt ttatgatttt
    20341 gcctgctatg ccaaagcgaa actgaacgtt gagcagctgc tttatcttga tggcacaatt
    20401 tcacatatgt atatgaaagg ctgggcaatc ccgtggcaac actatccttt tgttaccatg
    20461 atttccgtcg agtggaaagg ttaagcattg tacactcgag gaggccagac aaactgggac
    20521 tggctacaca gctggaaaat catcagataa atgaagggaa acaaaaatga aaaaaacgct
    20581 cgcaacgtta tttttattaa cgtgtctggg atcatcatcc gcctatgcgg ataacgcttt
    20641 gattttacaa actgatttta gcctgaaaga tggtgccgtc tcggcgatga aaggggtcgc
    20701 attcggcgtt gaccataatc ttaaaatctt tgatttaaca cacgaaattc caccgtataa
    20761 catctgggaa ggcgcttatc gcctgtatca gactgccagt tactggccgc aaggttcggt
    20821 gtttgttagc gtggtcgatc cgggcgtagg cactgaccgt aaatcggtag tgctgaaaac
    20881 caaaaacggt caatacttcg tttcaccaga taacggtacg ctcactttgg tggcacaaac
    20941 tctgggtatt gatagcgtcc gagaaatcga cgaaaaagct aaccgcctga aaggttctga
    21001 aaagtcatat accttccacg gtcgtgacgt gtatgcctac accggtgcgc gtctggcttc
    21061 cggggcgatt acgttcgaac aggtggggcc agagcttcct gcaaaagttg tcgaactttc
    21121 ttatcagaaa gcaaaagcaa caaaaggtga agtgaagggc aatatcccga ttctcgatat
    21181 tcagtacggc aacgtctgga gcaatatcag cgacgagttg ctgaatcagg caggaatcaa
    21241 actcaacgac acgttgtgtg tgaccatttc cgaaggttcc cagcaaaaat acgcagggaa
    21301 aatgccatat gtcgcaagct tcggcgatgt gccggaaggc cagccgatgg tgtatctgaa
    21361 cagcttattg aatgtttccg tggcgctgaa tatggataat tttgcgcaga aacatcaggt
    21421 tgcgtctggt gctgactgga atattgatgt gaagaagtgc gctaagtaag gtgctgatgc
    21481 cgaatgcggt gtggcgcacc gcatcctgca acggagttgt aaattacacc gtctccagtc
    21541 gggcataagc cgccaccagc catttaatac cctggccctg gaatgccacc tgcaaacggc
    21601 tatgctcgcc gctgccttcc atattgacga tggtgccttc accaaactta gcgtggcgta
    21661 cgcgctggcc gagcttgtag ccgctgtcgt tctcgaccat cggcgtaccc atccgttgat
    21721 ggctgaccgg gcggcttacc gtagcgcgca ggcgcacctc ttccacacac tcttccggca
    21781 actcaccgat aaagcgcgac gggcgatggt aaacctcttt gccatacaga cggcgagttt
    21841 ccgcgtaggt cagcgtcagt ttctgcatcg cgcgggttac gccaacgtag gccagacggc
    21901 gttcttcttc cagacgtccg ccttcatcca gcgacatctg gcttgggaac atgccctctt
    21961 ccataccaac gataaacacc tgcgggaact ccaggccttt cgccgagtgt agcgtcatca
    22021 actgcaccgc gtcctgccag gtatccgcct gcccctcgcc cgcttccagc gccgcatggg
    22081 agaggaatgc ctgcagcggc attaaatctt cgtcttcttc gttgtagctg aactggcgcg
    22141 ttgccgtcac cagttcctcc aggttttcga tacgcgtctg acctttttcg cccttctcct
    22201 gctcgtacat ggtgcgcaag ccggagtctt taattacccg gtcagtctgt acatgcagcg
    22261 gcatatcggc agtttcctgc gctaaggcgt cgatcagctc cataaaccgc tgtaaggcac
    22321 tggcagcacg tccggcgagg gctttttcct gcaacagttc acgacatgcc tgccagagtg
    22381 ttaactggcg atcacgcgat gtctgacgta ccacgtccag cgtccggtca ccaatacccc
    22441 gcgttggtgt attcaccacg cgctcaaagg ccgcgtcgtc attacggttg gcaatcaggc
    22501 gcagatacga gagcgcatct ttgatttcct ggcgttcgaa gaatcgcatc ccgccgtaaa
    22561 tacggtacgg catacttgcc tgtaacagtg cttcttccag cacacgcgac tgggcgttgc
    22621 tgcggtagag aatggcgcac tcggcaagcg ccccgccgtt gtcctgccag gttttgatac
    22681 ggttaaccac aaaacgcgct tcatcgagtt cgttaaaagc gcaatagagg gaaataggct
    22741 caccatccgc gccatcggtc cacagttttt tacccaaacg cccgttgttg ttttcaatca
    22801 gggcgttcgc ggcgctcaga atattgctgg tagagcggta gttctgctcc agacggatag
    22861 tttcagcacc ggggaaatca ttgaggaaac gctgaatatt ctccacctgc gctccgcgcc
    22921 agccgtagat tgactgatcg tcatcgccaa cgatcatcac tttgccggta tcgcccgcca
    22981 gcaggcggat ccacgcatac tgaatgttgt tggtatcctg gaattcgtcc accaggatat
    23041 tggtaaaccg ttcgcgatag tgctgaagga tatgcggctt gttaagccac aactcgtgag
    23101 cacgtagcag cagctcggcg aagtccacca gccccgcgcg gtcacacgct tcctgatacg
    23161 cctgatacac cttctgccag gtctgctcca ccggattacc gtagctttga atatgatgtg
    23221 gacgcaggcc ttcatctttc tggctgttga tgtaccacat tgcctggcgc ggcggccact
    23281 gcttctcgtc gaggttcatc gccttgatta ggcgtttgag caggcgtagc tggtcctcgc
    23341 tgtcgaggat ctgaaaatcc tgcggcagat tggcgtccat atggtgcgca cgcagcagac
    23401 ggtgcgccag cccgtggaag gtgccgaccc acatgccgcc ctggctggtg cccatcagct
    23461 gcccgatacg atgacgcatc tccgccgccg ctttgttggt aaacgttacc gccataatcg
    23521 agtatggcga gcagttttcc acgctcatca gccaggcgat acgatgcacc agtacgcgcg
    23581 tcttaccact gcccgcgccc gccagcacca gaaggttgct gcgtggcgcg gccaccgctt
    23641 cgcgctgttt gtcattaagg ctgtcgagca ggtaagaaac gtccattggc accgccgcgt
    23701 aaaaataggt tgggcggaga agcgcaacgc cctccgccaa aaagctgggt atatatacag
    23761 atttgctgat tatatcagcg aggtcagaga tgccaaccgg gaaatttcca gatgcgggag
    23821 taaacggctg tcccaggttt gcatcagatc gccattttcc ggtctgatcc aacaggcctg
    23881 cattccgctg cgaattgccc cacccacgtc agtggtgaga tcgtccccga catgtaagat
    23941 ctcgccgatc ggcacgttga gtttttccgc cgccaggaag tacatatcgc tgaacggttt
    24001 tgagcgcccg tgcgggccag cgcgcaacac aaactcaaaa taatccccta aaccaaacag
    24061 ctccggctgg gcgttaccgt tggtgatcgc caccagcggc catttcttcg ccagctgttt
    24121 taaggtgtcg tgggtttgct gcgggacatc gattcggctg cgccatttgg caaagttgat
    24181 cattgctgcg tgtgcgcctg cactggcttc ttcggcactc agcccggcgt cgagcatggc
    24241 ttgttcaatc gaacgaaaac gccagcgcgt cacgtcgtga taaatctcgg gttccgcttc
    24301 ccgtaccgcc tggcgcaggc gttgcagatc ttcattctgg aagctgcgca gcgccggatg
    24361 ataattttgc acaaaggtaa gcgcctctcg ctcggtgcgc aaaatcaccg gacggttatc
    24421 gtaaagggta tcatccaggt caaaggtgag cgccgagatg cgccccaaag gccggtaaaa
    24481 acgcattatt tcccccgttt ggcgcgtgga tgcgccgcat cgtacaccga ggcaaggtgt
    24541 tgaaaatcaa gatgagtata gatttgcgtg gtggagaggt tggcatgacc cagcagctcc
    24601 tgcacaccac gaagatcgcc gctcgactcc agcatatgcg tagcgaacga gtgacgtaat
    24661 ttatgcgggt gaacgtgatt attcagcccc tgttttattc cccattcggc aaagcgtttt
    24721 tgcacattac gcgcggagat acgcttgccc agtttcgaca ggaaaagcgc gtcgtcttcg
    24781 ctgccaaaca ggtcgcgcaa atcaagccag tgctcaatcc acgccacggc gttacgacca
    24841 atcggcaggc ggcgctcttt gctgcctttc cccatcaccc acacttcgcc ggactccagg
    24901 tcgaggtgtt tgatatcgag ccccaccagt tcggaaagac gcagccccgc gccgtacatc
    24961 acttccagca ttgcccgatc gcgcacggcg aggggatcgt tgatatcaat atccagcagc
    25021 cgattcatat catcgacgtc gatgtttttc ggcagatgac gcggcgcttt cggtgccgac
    25081 acacctttcg ccgggttggc tttgagttcg ttctggctga ccagccagtc aaaaaagcta
    25141 cgtagcgcag aaagccgtaa cgccagactt gcagctccca gccctttacg gcggctgcgt
    25201 acagcgaaat tgcgcaccat cgtcacatca cattgctgcc agctttgcag gccgttttca
    25261 ctggcaaaat tgatgatcgc ctcaagctga cgctgatagt taagtagggt tatcgggcta
    25321 agctgacgct ccacgctcag ataacgtagg tagcgttcta catcggtgtg taaatcggtc
    25381 atacgcgttc aatccaacgc tccagaagct ccggcaacat cagcgcaatt tcatgaagta
    25441 actgcgttcc ctgcccttgt tgatagtgac tggcatcgcg actggtaaac agcacgacac
    25501 ccaaatcagc atcgcttccc agcatcgaca tcgccaccga tcccaccgct ttcgcttccg
    25561 gtagcaccac cagaagctcc ggtccgttaa gtggccccag atagtgctgt tcctgcccca
    25621 gacgctgaat acgcagcggt tcgaaagact gacggcttaa tgccagatga gtgtggttcg
    25681 acggcgcacc taaacgccag cgatccggga acaggcgtag gctcgcaccc gccaggccga
    25741 gatcgcgcgc ccagcggtga aagcgcatca gcatatcatc gagactgctg gcggcggtga
    25801 gactgcgctg caggtagagt agacgataaa acaggccttc gttggcaata gcctgttcca
    25861 tcaacaacgc catgttctct tccagaacat gaatgtgatt acgtgcgcgg gccatgtgcc
    25921 attcgaccaa cgaaacggtg ccgcgtaccg gatgcggcac acgtatcgct tcaactgcgc
    25981 gcgcattgcg gataaaaaac tcaggatttt taatcagata atcgacaacc gcccggtcat
    26041 caagctccgt gagtgtttcc tgcagttctt cccctggttg cttcatagat gaataaatcc
    26101 gtcgtagaca tgtaccgccg ggccagtcat atataacggg tgacccggac ctttccaggc
    26161 gatatcaaga cgaccgccgg ggagttccac gcgtacttct tcggccagca aaccttgctg
    26221 aatccctacc gccaccgccg cacatgcgcc gctgccgcag gcctgggttt ctcctgcccc
    26281 acgctcataa acgcgtaaac gaatatgctc gcgcttaacc acttgcataa aaccgatatt
    26341 ggcgcgctcc gggaaacgtt cgtggctttc cagaacagga ccaagcgttt ctaccgccgc
    26401 ggtatcgaca tcatcgacct gaatcacgca atgcggattt cccatcgaca ccacgccgca
    26461 taagattgtc tgctcggcgg cgcgcataat ataggtcttt tccgctttgt tagcgcgaaa
    26521 cggcacggca gaaggttcga agttgggttc gcccatattt acgcggacca gatcatcatc
    26581 ggtgacggta agaaccatcc gcccgttggc ggtgctgacg cggatatcgc gcttattggt
    26641 cagccctttc agacgcacaa aacgggcaaa gcagcgtgct ccgttgccgc actgcgccac
    26701 ttcactgcca tcagcattga aaatgcgata gtgaaaatcc agttcaggat catacggcgg
    26761 ctcaaccacc agcagttggt caaaccctac ccccaggtgc cgatcagcca ggcgacgaat
    26821 cagctccggt gaaaaaaaga cattctgcgt taccgcgtcg acgaccataa aatcgttgcc
    26881 aaggccatgc attttcgaga actgcatcat ttactccaat cacgcgggta cagaaactga
    26941 cttttagtaa ttcacctggg atggaccatc gccagtggcg cggtcgtttt tatccggcac
    27001 cgtggattgc gtttgcgtct ctaccggttt ggtcggtggc ggtgcgtttt tatctgcagg
    27061 cgggaaatag agcggacctt tcagaccgca gcccgtcagg ctgaagagag taagtaatac
    27121 agtgagtgcc ttaaacacgt ttttcattat ggattgcctg taaagttcat cgctttctgc
    27181 tttctatcat cgcaggagaa gacgtaaaag caagcgttta gcgactcttt atgaagtctg
    27241 aaaagtgagg aaatgctgcg agtataacgc ttacacgtgg cgatttatgt tttgctggca
    27301 gtttcgtgat cggcacctcg cgccaggatg gctttatgca ctatcgcaac gtggtttgtt
    27361 tgttgtcttg ttcgttgttc ttatcgtcgg catggggatg tcggctttcc gcgcctgaac
    27421 ataatattta ccagaaacag ggaaaaggcg tggtgtacct gcgtccttat gaaaagacca
    27481 atctttcgct atatagagat attattattc cgttgacgga tgaatcatgc cgtaacgcag
    27541 ccgcagacgg gcaaactttg aagccctgac cccataccgc tatactgccg ccatcactta
    27601 aaaacaggat acaacaatga acgacagtga atttcatcgc ctggctgatc aactgtggct
    27661 gaccattgaa gagcgtctgg acgactggga tggcgatagc gatatcgact gcgaaatcaa
    27721 cggcggcgtg ctgaccatta cctttgaaaa cgggagcaaa atcattatca accgccagga
    27781 accgctgcac caggtatggc tggcaaccaa acagggcggc taccattttg atatgaaagg
    27841 cgatgagtgg atttgcgatc gtagcggcga aaccttctgg gatttgctgg aacaggcggc
    27901 aacgcagcaa gcgggtgaaa cagtcagttt ccgctaagat tgcatgccgg ataagcctcg
    27961 ctttccggca cgctcatcac gaaaaatact gctgtaatag cggcgtgtcg tgatcctgat
    28021 tggcaggcgg catgttgcca atcgatttgg tgcggaacgg aatcacctgt tcacgaccat
    28081 caaccttcac aatctgatag aactgcggca ggttgaagtt gatgaagctt gagccgtagg
    28141 taaaacggtc gtgcgatgac gagtagaagc gactgacgtc acgcaccagc tcctctttgc
    28201 tgccttcgca gtggtgatac acctcaaccc ggttgctttc gtcgagaatg tagatattaa
    28261 agccattctc gtcttgcgtt tcttcgaaga agaactggat gatcccttcg ctggcaaagc
    28321 cgtccaccac cgccggtaat ttgacgtgat tggtttcaac ctgcactgac aggccgtgca
    28381 gtttgttatg cgaaatagcg ccataaaact cgatggcatt ttctagtttc tgtaccgata
    28441 cattcaggcg ttcgaagaat aagccccagg tttgaccaga aacgcgcagc gccttgaaac
    28501 gtccggtttc ctggcgggtg ctggaaagac gcagttcaat gcactcagaa accagttgct
    28561 gcacgcgagt acggattaat ccgcgcagat gctggctata acagaagact tccacgctat
    28621 ctggcggtgc ggcgtcctga tgcattttac cgagaatggt tttcagggct tcgatcatcg
    28681 actgctcgcc gttgaagtgc aacgtacgca cttcgttcca cgagttgcgg tacagcaggt
    28741 ccacgctacc taccaggcaa ttttgattct cgccaaagct gaagacgtcc agcttacgga
    28801 aatcaaaatg caccacctga ttgcggaacg cagctgtcgg gtcatattcc aggttaacga
    28861 taatcgccag atggcgaatc tcacatgggc tgtagagcgc ttttggcgtc ggtgcaggca
    28921 ggcgcagcgg gaaatggtgc gacacgtcgg cgaccatctc ctgcaactta ggcaaatcga
    28981 caatgccgtt gcctttaata tacaagcggg tgcgcgaggt cagcaggccg ttaaaccatg
    29041 cccacgccac cagtttattc aggtaacggt tatattccag cggctgatgg ctgatgatcg
    29101 actcaatatt tggcgcgcgg ttatacagat accaacctga acggttagcc cggcccggcg
    29161 gcacataaat aaaggtcaga ttcggttccg agagatcggg cgaaatctgc gggtttacca
    29221 gcgtcacttt acctggcaat gcttcaaacg cggcgtacag cttacgtgtc agcacgccga
    29281 tatcctgcgg gctggcggag acgctaaggt tattgcggcg cgcaaagcgg atcagattac
    29341 ggtagctctg catcatcgcg tcgagcagct cgttgtgcgc ctcacgcacc tgatcaatct
    29401 tccagttggc gcggttatcg agcattgcca gacgcgcatc gtcccattcc cactctttca
    29461 ctaactgggt tagcacttca cgacgccagc ctacgcaagc gcgttcgcgg ctgagttttt
    29521 cgcacacttt taaatagaag cagcggcgta ctaaatccag acgggtgaaa tcttcaatcg
    29581 ccgtcaggta ttccgtaaca cgctccagca tcatgcagta tggatcgagg ccaaacgaca
    29641 caatctcgcc gtcgtgcaaa cgctgtttga tatctttcgc cagcaggcgt gggttgggat
    29701 attcccagga ataggcttcc aatagcagtg ttttcagcac cgctttgtat ggggaatcga
    29761 tactcttgta gagctgccaa aggctggcac caaagtactc ttcagcagaa agcgagctta
    29821 agccaccaag atccagccat tcattaggag taagcacgcc ctgcgcgtaa agcgtcatca
    29881 catagtcgtc gtaatgctct tcttcgtcgc acggcaccat attccacaga atacgcttac
    29941 cggcgagacg cacggcggta cgataaaatt cgtcaagcag cagtatatgc tgggtggagc
    30001 cacaatcttc gccccccagg ctgccgcttt cattatgacg gaagcgattt tcatcaatca
    30061 ggaagaagct gacttccaca cccagcgagg cggcccagct ttccagcaga ctacatttac
    30121 gttgtagtaa ctggcgctct tcgctatcga gccaggattg atgacagacc cagatatcca
    30181 ggtcagagga acaactttgc cctacggacg aggtgctgcc catggtgtat acaccagtaa
    30241 ttggaagctc acctttcggc ggatcctgta ctgacattcc acgatacagt tcaagctcgt
    30301 tcaggtagtg gcgttgagtt tcatcaggcg tgtaaaggca aatgcctttg ggaacgttac
    30361 catcaaggta acccggcatt agcggatggt gatagtgcaa caatgtcggc agtagactgt
    30421 agacctgttg gaatgcaggc cccatagcag caagcgcgcg atccacacgc aattgattta
    30481 tggcatccag tctctgtttc agagtctcaa tatagaggta caagacgtat cgcctgattt
    30541 gctacccgtc atgactgtga ttccgccaac atcaacggta acacgcggca ttcgggatat
    30601 ttcgtatgtc aaaggtaacc gttaccactt ttcgcgcctg gtttttttag tttcacgacg
    30661 aaaaaatggt ctaaaacgtg atcaatttaa caccttgctg attgaccgta aagaaagatg
    30721 cgctacatac aagtgtagca ccgtttattc tctgtaaatt ccttattacg acggcgtgaa
    30781 acgcctgtca ggatccactg ccagacctca ttttacggtt tgcgcaggcg tctacgtttc
    30841 accacaacac tgacatcgct ctggcaagga tgttaggatg gaccacggat gataatgacg
    30901 gtaacaagca tgttagacaa tgttttaaga attgccacac gccaaagccc acttgcactc
    30961 tggcaggcac actatgtcaa agacaagttg atggcgagcc atccgggcct ggtcgttgaa
    31021 ctggtaccga tggtgacgcg cggcgatgtg attcttgata cgccgctggc gaaagtaggc
    31081 ggaaaaggct tatttgttaa agagctggaa gtcgcgctcc tggagaatcg cgccgatatc
    31141 gccgtacatt caatgaaaga tgtgccggtt gaattcccgc aaggtctggg gctggtcacc
    31201 atttgcgagc gtgaagatcc tcgcgatgcc tttgtgtcca ataactatga aagtctggat
    31261 gcgttaccgg caggcagtat cgtcgggacg tccagtttac gtcgccagtg ccaactggct
    31321 gaacgccgcc cggatctgat tatccgctcc ctgcgcggca acgtcggcac tcgcctgagt
    31381 aaactggata acggcgaata cgatgccatc attcttgccg tagccggact aaaacgttta
    31441 ggtctggagt cccgcattcg cgccgcattg ccacccgaga tttctcttcc ggcggtagga
    31501 caaggtgcgg tgggtattga atgccgcctt gatgattctc gcactcgcga gctgcttgcc
    31561 gcgctgaatc accacgaaac tgcactgcgc gttaccgcag aacgcgccat gaatacccgt
    31621 ctcgaaggcg gatgtcaggt gccaattggt agctacgccg agcttattga tggcgaaatc
    31681 tggctgcgtg cgctggtcgg cgcgccggac ggttcgcaga ttattcgcgg tgaacgccgc
    31741 ggtgcgccgc aagatgccga acaaatgggg atttcgctgg cagaagagct actgaataac
    31801 ggcgcgcgcg agatcctcgc tgaagtctat aacggagacg ccccggcatg agtatcctgg
    31861 tcacccgccc gtctcccgct ggagaagagt tagtgagccg tctgcgcaca ctggggcagg
    31921 tggcctggca ttttccgctg attgagtttt ctccgggtcg acaattaccg caacttgctg
    31981 atcaactggc agcgctgggg gagagcgatc tgttgtttgc cctctcgcaa cacgcggttg
    32041 cttttgccca atcacagcta catcagcaag atcgtaaatg gccccgatta cctgattatt
    32101 tcgccattgg acgcaccacc gcactggcat tacataccgt aagcggacag aagattctct
    32161 acccgcagga tcgggaaatc agcgaagtct tgctacaatt acctgaatta caaaatattg
    32221 cgggcaaacg tgcgctgata ttacgtggca atggcggtcg tgagctaatt ggggattccc
    32281 tgacggcgcg cggtgctgag gtcatttttt gtgaatgtta tcaacgatgc gcaatccatt
    32341 acgatggtgc agaagaagcg atgcgctggc aatcccgcga ggtgacgacg gtcgttgtta
    32401 ccagcggtga aatgttgcag caactctggt cgctgatccc acaatggtat cgtgagcact
    32461 ggttactacg ctgtcgacta ttggtcgtca gtgagcgttt ggcgaaactc gcccgggaac
    32521 tgggctggca agacattaag gtcgccgata acgctgacaa cgatgcgctt ttacgggcat
    32581 tacaataact ctcataacag gaagccataa tgacggaaca agaaaaaacc tccgccgtgg
    32641 ttgaagagac cagggaggcc gtggacacca cgtcacaacc tgtcgcaaca gaaaaaaaga
    32701 gtaagaacaa taccgcattg attctcagcg cggtggctat cgctattgct ctggcggcgg
    32761 gcgtcggttt gtatggctgg ggtaaacaac aggccgtcaa tcagactgcc accagcgatg
    32821 ccctggctaa ccaacttact gcactgcaaa aagcccagga gagccaaaaa gccgagctgg
    32881 aaggcattat caagcaacaa gctgtacagc ttgagcaggc gaatcgtcag caagaaacgc
    32941 tggcaaaaca gctggatgaa gtccaacaaa aggtcgccac catttccggc agcgatgcta
    33001 aaacctggct gctggctcag gctgatttcc tggtgaaact cgccggacgg aagctgtgga
    33061 gcgatcagga cgtcacgacc gctgcagcgt tgctgaaaag tgcagacgcc agcctggcgg
    33121 atatgaatga cccgagtctg attaccgttc gccgggcaat taccgatgat atcgccagcc
    33181 tttctgcggt atcgcaggtg gattatgacg gcattatcct taagcttaat cagctttcaa
    33241 atcaggtaga taacctgcgt cttgctgata atgatagcga tggttcgccg atggattccg
    33301 acggtgaaga gctttccagt tccatcagcg aatggcgtat caatctgcaa aaaagctggc
    33361 agaactttat ggacaacttc attacgattc gccgtcgtga tgacaccgcc gtaccgctgt
    33421 tagcgccaaa tcaggatatc tatctgcgcg aaaatattcg ctctcgcctg ctggtcgcag
    33481 cacaagctgt accgcgtcac caggaagaga cttatcgcca ggcgctggag aacgtctcca
    33541 cctgggtacg tgcttactac gatactgatg atgccaccac caaagcgttc ctcgacgagg
    33601 tggaccagtt aagccagcaa aatatctcga tggatcttcc ggaaaccctg caaagccagg
    33661 cgatgctgga aaaattgatg cagacccgcg tgcgtaacct gctggcacaa ccggcagcag
    33721 ggacaacgga agctaaacct gcacctgcac ctgcacctgc accgcaagct gatactccgg
    33781 cagccgcgcc gcaaggagaa taaccatgct aaaagtgtta ttgctctttg ttttgctgat
    33841 tgcggggatc gtggttggcc cgatgattgc cggtcatcag gggtacgtat tgattcagac
    33901 cgacaactac aatatcgaaa ccagcgtcac tggcctcgcg attattttga ttctggcgat
    33961 ggtagtgctg tttgccattg agtggctact gcggcggatc ttccgcacag gcgcgcacac
    34021 ccgtgggtgg tttgtcggac gtaagcgtcg ccgtgcccgt aagcagaccg aacaggcgct
    34081 gctgaaacta gcggaaggcg attatcagca agttgaaaag ctgatggcga aaaatgccga
    34141 tcacgctgaa caaccggtgg tgaactatct gctggctgcc gaagccgcgc aacaacgcgg
    34201 tgatgaagca cgcgccaacc aacatctgga acgcgcagcg gagctggccg gcaacgacac
    34261 aattccggta gaaatcaccc gtgtacgtct gcaactggcc cgtaatgaaa accatgctgc
    34321 acgccacggc gtggataagc tgctggaagt tacgccacgc catccggaag tattacgtct
    34381 ggcggaacag gcgtatatcc gcactggtgc atggagttcg ctgctggata tcatcccatc
    34441 aatggcgaaa gcccacgttg gcgatgaaga acatcgtgca atgctggaac aacaggcatg
    34501 gattggcctg atggatcagg cgcgtgccga taacggtagc gaaggtttgc gtaactggtg
    34561 gaaaaaccaa agccggaaaa cgcgtcatca ggtggcgtta caggtggcaa tggcggaaca
    34621 ccttattgag tgtgatgacc atgacaccgc ccagcaaatt atcatcgacg gcctgaaacg
    34681 ccagtatgac gatcgcctgc tgctgccgat cccgcgtctg aaaaccaata acccggaaca
    34741 gctggaaaaa gtgctgcgcc aacagatcaa aaacgtgggc gatcgcccgt tgttgtggag
    34801 cacactgggt cagtcgctga tgaagcacgg cgaatggcag gaagcatcgc tcgccttccg
    34861 cgcggcgctg aaacaacgtc cggacgccta cgattacgca tggcttgccg acgcgctgga
    34921 cagactgcac aaaccggaag aagccgcagc gatgcgtcgc gatggcttga tgctgacctt
    34981 acagaataac ccgtcacagt agttctttct cacccggagg caagcacctc cgggaccttt
    35041 ctgatacata aaaaaacgcc tgctcttatt acggagcagg cgttaaaaca ggtctgtatg
    35101 acaacaagtg ggtgcttcac tcaacgttgt gtccatggtg tctgatgagg cataagcgac
    35161 atctgtcagt ggacgataag caccgtaaac ggctctgcgt cattccggag tttatgaggc
    35221 actaaggcga acataagaga tggaatgagc atctactcgt ttattatgcc acagagaacc
    35281 gggaaataac atcccttaac acttgttatg agataattct gtaatcctct ttgcttcctg
    35341 agtgaatatt tctcctgcgc acgattctac acatatatat aatgaataat aaaattcatt
    35401 caattaataa catatatatt aattgccgtt aaaactaaaa acagcatcaa taatcaacgc
    35461 gatataacat caccgcctta catatcacct gcgccagagg taggattgaa aacgctctcc
    35521 tgattttcca attcattttc tggataaata aataatttat ttttatcact attatttatg
    35581 caatcatcct gtcagggaga gggatctcaa ttatcaatgc ttaattacgt caacattttg
    35641 acgacatgaa accggagcat tttgcatgga attttcgttt tcacccaaac ttcttgttgt
    35701 agctgtcgcc gccgctcttc cactcatggc caacgcagca gataccccgt caaccgccac
    35761 cgcacgcaaa ggctttgccg gatacgatca cccaaaccag tatctggtta aaccggcgac
    35821 cactattgcc gacaacatga tgccggtgat gcagcatcct gcacaagaca aagaaaccca
    35881 gcagaagctg gcagaacttg agaaaaaaac cggtaagaaa ccgaacgtgg ttgttttctt
    35941 gctggatgat gtgggctgga tggatgttgg ctttaacggc ggcggcgtgg cggtgggtaa
    36001 ccctacacca gatatcgacg ccgttgccag ccaggggctg attttaactt cggcgtattc
    36061 tcaaccgagt tcttccccaa cccgcgccac gattcttacc ggacaatact ccatccacca
    36121 cggcattctg atgccgccaa tgtacgggca accgggcggt ctgcaagggt taaccactct
    36181 gccgcagttg ctgcacgatc agggctacgt cactcaggcc atcgggaaat ggcatatggg
    36241 ggaaaacaaa gagtcgcagc cgcagaacgt tggctttgat gatttccgcg gctttaactc
    36301 ggtatccgat atgtataccg aatggcgtga tgttcacgtc aacccggaag tcgccctgag
    36361 cccggatcgt tctgaataca tccaaaaact gccgttcagc aaagatgacg ttcatgccgt
    36421 gcgcggcggt gaacaggaag ccattgccga cattacgccg aaatatatgg aagacctgga
    36481 tcaacgctgg atggaatatg gcgttaagtt cctcgacaag atggcgaaga gcgataagcc
    36541 tttcttcctc tactacggca ctcgcggctg tcacttcgat aactacccaa atgcgaaata
    36601 tgcgggtagc tctccggcgc gcacctctta cggcgactgc atggtggaga tgaacgatat
    36661 cttcgccaat ctgtataaag cactggagaa aaacggtcag cttgataaca cgctgattgt
    36721 gttcacctct gataacggtc cggaagccga agttccgccg cacggtcgca cgccgttccg
    36781 cggtgcgaaa ggttctacat gggaaggcgg cgttcgtgtg ccaaccttcg tttactggaa
    36841 aggcatgatc caaccgcgta aatctgacgg cattgtcgat ctggcggatc tcttccctac
    36901 ggcgctggat ctggcggggc atcctggggc gaaggtagcg aatttagtac cgaaaaccac
    36961 ctttatcgat ggtgtggacc agacatcttt cttcctggga acaaatggtc agtctaaccg
    37021 taaggccgag cactacttcc tcaacggtaa actttctgct gtgcgtatgg atgagttcaa
    37081 gtatcacgtc ctgatccagc aaccgtacgc gtatacccag agcgggtatc agggtggatt
    37141 caccggcaca gtaatgcaaa cggcgggatc gtcggtgttt aacctgtaca ccgatccgca
    37201 ggaaagcgac tccatcggcg tgcgccatat tccgatgggt gtaccgctac agaccgaaat
    37261 gcacgcgtat atggagatcc tgaaaaaata tccaccacgc gcgcagatta aatctgacta
    37321 agccggcgct atcgctgaga tctgcctttg ccggatgcga tgctgacgca tcttatccag
    37381 cctacagaac gctgcaattt attgaatttg caaggattgt aggccggata aggcgttcac
    37441 gccgcatccg gcaatcaacc gcaggcggcc gccgatttct acttattcac caccatcaaa
    37501 tgcgcctgca taatgtcgct ggccggacga ccgtgcgcca gcaaatcagc cattgcttta
    37561 agatatggcg gtagatggcg gaaataacgc tgatacccgg cacacaaata attcagcccc
    37621 ggtttgccgc tggcatcgag cataaagcgg tgtttcgggc agcctcccca acatgctttt
    37681 aacacgttac aactgcgaca ctgcgctggt aactgcttaa atttatcttc accaaacacc
    37741 tgctgttgcg gggaatctac catttctgca attgtctgct gaagcatatt ccccagtcga
    37801 tattgcgaat aaacatagtg atcgcaggcg taaacgtcgc cgttgtgttc aacaatcacc
    37861 gagcgcccac aggttggctg atgatggcaa accgctcctg gcgcaccgac aaaattggca
    37921 aacgcccatt cgatattcat cacgaaaatc ttgccgacgt cgcgtttgat ccagtggtcg
    37981 aatatcgcca ccaaaaattc accgaactca tcggggcgta ccgaccattc cgtcagttcg
    38041 ccctgaatat cgccgggagc atgtagctta agtccagcat gaacagccgt ttcatcagcc
    38101 aggcgttcga ccacaggaat aaactggata aactcaacgc ctgcatcaca cagaaaatca
    38161 tagacttgca aaggttgtaa cgcactggtg cgatttacgc agaccagcac gttatagttg
    38221 acatgatgtt tttgcaggag cgtcagggca cgcatcacca gcttatgcgt tggtctgccg
    38281 cctttggtca cgcgatattg attgtggatc tcagccggac catccagcga taacccaaca
    38341 agaaaatgat tttctgccag aaatgcacac cattcgtcat caagcagaac gccgttagtc
    38401 tggaagctgt tacttatctt cctgccagca ccatatttcg cctgtagcgc cacggcacgg
    38461 cggtaaaaat ccagcccgag taacgttggt tcgccaccct gccaggtaaa agccacttcg
    38521 ttttgggttt cgctggcagc gatatagtga cggacatacg cttccagcgt gtcatcgtcc
    38581 atatgcgtga ctggcttttc gcggtaaagg gattgttttt cgagataaaa atagtagtca
    38641 cagttcagat tacaatcgga accgctcggt ttcgccatca catgaaaagc acgcgttgga
    38701 acctgttgca gcatggtcgc tccttgcctg aagtcatcct tcaataagtt aagggcgaaa
    38761 tcataccgtg gcgcaatgcg ttattacggg cgtttattgc gaagggaaag tgacgaaatg
    38821 cacagaaaac aaaaaacccc gccgaagcgg ggttcaaaat tggtcggcga gagaggattc
    38881 gaacctccga cccactggtc ccaaaccagt tgcgctacca agctgcgcta ctcgccgaaa
    38941 tactgctttt tgaattttta gttcaattct ttaaagtcgt ggtgcgaggg gggggacttg
    39001 aacccccacg tccgtaagga cactaacacc tgaagctagc gcgtctacca attccgccac
    39061 cttcgcattg tcacaacttc taataatggg gtggctaatg ggattcgaac ccacgacaac
    39121 tggaatcaca atccagggct ctaccaactg agctatagcc accactacaa atcttgttac
    39181 gcggtattac taccaccgca gctcaagcgc cgggactaaa tggcgcgccc gacaggattc
    39241 gaacctgaga cctctgcctc cggagggcag cgctctatcc agctgagcta cgggcgctta
    39301 gcgccgttgc gggggtggat aatacggact tcccacccct ctgtctagtc cctttttaaa
    39361 taaattgcgc gtttggttac ggtttgtgcg ttttgctgct tattcctcca gtttatgcgc
    39421 tttaccgtgg cgattaagcc caaaaatttt ataaatcgcc gtcaccgcca gcataaagat
    39481 gatgccgaca aacagcgaca tacgcgtatc ttcattaaag tacatgccga tcaaaacgca
    39541 aatcaggaat gccattgtta cgtagttggc ccacgggaac aggattgagc ggaacggatg
    39601 gctggcaatc gccgctttat gcgcacgacg aaaacgcagc tggcttatca atatcacaaa
    39661 ccatggcacc atccccggaa gcacgctggc actgtagacg tagacaaaca cacgctgcgg
    39721 attgggaatg atgtagttca ggcatgagcc aattaacagg atagcaatag atactgccac
    39781 acccgcaacc ggtacgccgt gacgggaaac tttcgccatt gccgccggta actgacggtt
    39841 tttcgccagt gcgtagagca tacgtccgca actgtacatg ccgctgttac agccagagag
    39901 cgcagccgtc agcaccacaa agttgataat acccgccgct gcggtaatgc cgattttggc
    39961 gaaagtcagt acgaacgggc tgccgttgct gcctatttca ttccacggga agatggtgac
    40021 gataacgaaa atcgcgccta cgtagaaaat caggattcgc cacagcacct tgcctacggc
    40081 actgcgcagc gtcacctgcg gattcttcgc ttcaccggca gtaatgccaa tcagctccac
    40141 gccctggtag gacgccacca caatacacag cgcggtcagg aaccctttcc agccccccgc
    40201 aaagaaaccg ccatgctctg tgagattgct aaaaccaatc gactgcccgc cattgccaaa
    40261 gccaaagaaa atcacgccca ggccaatgac aatcatcacg ataatcgtgg tgactttgat
    40321 catcgcaaac cagaactcga tttcgccgta caaccgcacc gccgccagat tcgccaatgc
    40381 taccagcgcc accgcgatca atgccggtat ccattgcgcc atctccggga accagaactg
    40441 gacataaacg ccaatggcgg tgatttctga gatccccacc gccatccaca taaaccagta
    40501 agaccaggcg gtgagatagc caaaaaacgg gctcatataa cgatgcgcat aaacggcgaa
    40561 cgaaccggta acaggttcga ggaacaacat ttcgcccatt gaacgcatga tgaaaaagac
    40621 gaatagcccg gcgatgatat aggccaacaa tacggatggc ccggcccatt tcagggtact
    40681 ggcggccccc ataaacaggc cgacgccaat ggtgcccccc agggcgatga gttcgatatg
    40741 tcgagcctcc agcccacgct gtagctctgg tttgttatct gccataaatc ctcgtgttgt
    40801 gtttgcatgc tttccggtgt taccggttat cgttatgggt acatcgagtg ttgcgaatgt
    40861 tttcgtaatt caggagaaat ggcaaataaa gcattaaaaa tttgaatgct ttgtgtaata
    40921 aaaaagcagg aaggcgacga agtgaccact ccgtcgcttt acaaagagag gaaaatcaga
    40981 ggttgccggt gtagtgccag cgtaaataac gcagcaaacg aagctgacgc ttaatgcggc
    41041 tcggctgcga aagcaggcgg tagagccact ccagccccag cgtttgccag attttcggtg
    41101 cgcgttttac gtgaccggtg aaaacatcgt aagtcccgcc aacgcccata tacagcgcat
    41161 ccggatgcac cagacggcag tcgcgcatga agatctcctg ctttggcgat cccatcgcaa
    41221 cggtgacgat ttgcgctccg ctggcatgaa tgcgttcaaa cagcgcctga cgctgctcgg
    41281 gtttaaaata accatcctga ctgccaacga tattcacatt ccactggttg cgcagtttag
    41341 cttcagtttg cgccagcact tcaggtttac cgcccacaag aaataccggc gtcccttctt
    41401 tgcctgcgcg cgccatcagc tcttcccaga gatcggcccc ggcaacgcgg gaaacctgcg
    41461 cctgcgggta ctttttacgt actgaacgta caacgctgat gccatccgca tatttaaatt
    41521 cggcagcgtt aattaactcc ctgacctcag cattatcttc aatggtcagc attttttcag
    41581 cattaatggc aaccagcgtt ccctgcttaa gctgcccgtc agcgaacaga taatcgaggg
    41641 cgtgctgcat atcacgccaa ccgatcaact gtaagccacg cagcgtatag gtcggtgccg
    41701 tggtgttgtt attcattgtt atccttcaac ctgcgtccgg agcgatgatt ttgttcgttt
    41761 atgaatgagt ccggcgcttt caaaaagcca gtacaacagt tttgcgatca tcagacatgc
    41821 gccgaagacc acgataaaaa agaccacgcg tgagacaaac gaatccagcc cttcacgcgc
    41881 cagcacgatc atattgaaga tcgccccgaa gcagaaactg tgcaatatcg cagccttata
    41941 gcgattagtc tcgcggttgc ccagctcata cagccagtcg aaccatttga tgatcagtcc
    42001 aacaacaatc gccccgagcg ggatgaacaa cgcgccgccc atcaccacca gtgagcctat
    42061 aagcgtaggc gagatcgcca gtccggagtg gttattcagc acttcccagg taaagtagtt
    42121 ggcggagttc agtaccatac tcgggcgacc cggccacagc caggaaggga taaagacata
    42181 gaaatcgcgg acaatgggag ccaggccctg gaagtcgatg ttgtcgtagt tctgcagcag
    42241 caatgccaga ttctcccacg gcgaaaaggt atcgcgggtg agatagagaa acgtatagaa
    42301 cgcttcatca ccgctcacat tcattccata gcgttttagc gccagccaga acatgcccac
    42361 aatccccagc acgcccgccg ccgccagcat ccacaacgaa atccagccgc gaataatgcc
    42421 aataaacagg aagatagcga atgcgatgat gatattggcg cgagtgccgc cgacaatcat
    42481 ataagtcagc aagccaaaag cgaccgtgct gacgaggaaa aacagccacg ctttgctgtc
    42541 ctggcgcaga aagtagacca ccagcatcgc cgggatgaaa aagtaaaaga agcgttttaa
    42601 tgccacgccg gagacttcgc tggaaaagat ctgactgtaa gaattaagcc ggaacagcaa
    42661 aaagccgttg tgcatgaaga agatgccgac gctcaccagc gcgatgccca tcaggatcac
    42721 ccacgtgaga ttggtctcca cgcggttcat ggtaaacagc ggacggcgcg gtgcatcagc
    42781 aacgcgtttg cgtagacggg ttttgtaggt gacatagtaa accgcgtaga agcagcctgc
    42841 agaaagcaac gcctgcaaca agatttctgg cggcgcgaca ccaacatcaa agcgaaatac
    42901 cagcacgctg gtcagcggaa agccgaagaa aaaggtgagc aaaaacagca atgaaaagaa
    42961 gacattgaag ttaaagcgca cacggcgaaa ctcaaaccag gtcagcgtgg caataaacag
    43021 cgtgcagagc agccagacaa caaaaaggcc actgaattgc agcagactca tgcgacctcc
    43081 ccggtggcaa tcgccaacgc ccgctgccag ccttgtagat agttagggct aaagaaggca
    43141 atggtgtttt tatccaccga cgccagctgg cgctgcgctt cacgcacaat atcctcgttg
    43201 agatcgtcgg tagtaaacag caccggcaaa tgttgttccg tcatatcctg ccagaacgga
    43261 ttttcccggt taagcacaca aggaatgccc gcctgaatca gtaagcacag cgtaccaatg
    43321 ccctgctggc gggcaaaaat aaagtaacca agatcgcact gacgaagtag cgtcagatag
    43381 gcgtcaaatt ccagtttttc gctcagaact tgcagatttt cttcgctgaa taactccagc
    43441 cccgcctgac gaacttcctc gatgtacgct tcgttattag gcggatatcc catcggcacc
    43501 accactttta ccgtatcgcc aaattgctga tgaacggcgc gcaaggcagc aatatgctca
    43561 ttgctgcggt cgccggagtt gcccaccaga atggtcattt ttccttcacg ttgccgatcg
    43621 ttcgccatcg tattgagaga aggatccatc cgcgtcggga agtacagcag ttcgccccgc
    43681 acctttgggt gcgttttggc aaaaaagctc aaatcaccgc gggtggcaaa tacacagccg
    43741 actcgctttt gcgccaggcg acgtagtggg taaaaaagct tatatctcaa gccactggaa
    43801 agctcgtaca ggtctgcccc ccagatatgc cagtaaaact ggctgggctt aataccacca
    43861 ctcagcagag ccagccacag tgtgggattg aactgaccgt ggaaaaaaaa acgctgctga
    43921 cggttagctt ttgctttcgc gatgaccgct tccgccagcg attttttccc agggaaaaat
    43981 tgcacagaaa gcgtcggaca gctatcactt aaaccgtcgt ccttgccaac aaccataaac
    44041 tcgcgcgcgt gctcgctcgt cgcggccagc gcgtcattga aaaaccgcaa aacggttcgg
    44101 ttatggtgag ggatatccga tcccagtacg tgaatcagta cagtcatgcc cgcctacgcc
    44161 agagtaaaaa cacgccacaa caaagagaaa aatagacgat ataggttgcc atataggcct
    44221 gcgccgcgcc cagtgcacca tgcgcaggga ttagccagtg ggaaaatacc atcaataaag
    44281 tgaactggct gacttccgcc agaatataaa accgcagtga cgctttggcg atcaccagat
    44341 aaccaaagac ataagcgccc acttttaaca catcacctac caactgccag gcaaagagat
    44401 cgcgcatagc ggtaaattta ttcgacaaca gcagccagat agcaaaatca cgcagcaacc
    44461 agacggaaaa actcgccgcc gccactgccg gtaagacgaa tttcagcgat ttaaccactt
    44521 cccgggtgat atcgcgcttt tccgttagcc gcgacaacgt gggcagcaaa tatacgctga
    44581 acgatgccgt aataaattgc aggtaggcat cggaaatact gctcacccct tgccagatcc
    44641 ccacttcatc ccagctatac tgcgccgcca gcagtttacg catcatgatg taagcaacag
    44701 gcaaggttac cgacgtaatc aacgccatga gcgtaaattt gctcaactgc cctgccaggc
    44761 cgttatccca gctgggtttc agatagctta acgggattgc accacgctta attagcatga
    44821 tgacggcagg aatcaccacc agcgcgggaa tcaacgccag ccccagcaac gccccttcat
    44881 aaccgcccaa acggtaactg acgtagtacg caagaacgcc aatcaggctg ccgacaatca
    44941 gggataacgc attacctgcg gcatcgcgaa agcctttcat caacgccagt aacaggttgc
    45001 cccaggcgat ccccatttgc accaacgcca ctaaacgcac cagcccctga tagtcggtat
    45061 taccgaacaa tccctggctg attggcgcag ctgccagcac aaaaaccagc gccattagca
    45121 tagagaagcc aagtaccatc gctgatgaag tgccgaccac gcggcgcaat tgttggggat
    45181 tatcatggta ctgggcaacg tatttggtta cgccgttaaa gatgcctgcc ccggcaagca
    45241 cgccgagcac ggtaatcaac tggcggaaat ttgccgccag cccaagcccc gccggaccaa
    45301 atgacactgc cagcaactta ccgaccagta acccggcacc aatcttgacc agtgtactgg
    45361 ccgccgtcca caaggacgct tttgccaacg acatatcagg agaagtagtt caacaaggtc
    45421 gcaattaccg tacgctgatt gacgggcgac aggttgtaga acaacggcag gcgcagcagg
    45481 cgctcgctct ctttggtggt gtagcgatct tcaccgtgga actcaccaaa gcgctccccc
    45541 gcagggcaac cgtgcagcgg aatgtaatgg aacaccgcca tgatttccgc ttctttcaga
    45601 aagttaatca acgcgctccg gtcatcaata tcccgcagtt taatgtagaa catatgcgcg
    45661 ttctgcacgc agccatcggg aatcgacggc agctcgatac gcccggcttt cgccagaggc
    45721 gctaacgcat cgtagtagtt ttgccacagc gccagacgct gctgattgat acgttcagcg
    45781 gcttccagtt gcgcccaaag gtacgctgct tgcagatcgg acatcaaata gctggagcca
    45841 atatcgcgcc aggtatattt atcgacctga ccacggaaga actggctgcg gtttgtgcct
    45901 ttttcacgga tgatctcggc tcgttcgatc aacgctttat cgttaatcag cgtcgcgccg
    45961 ccttcaccgc ccgccgtgta gtttttggtt tcatggaagc taaagcagcc aatatgacca
    46021 atggttccca gtgcacgccc tttgtaagtg gacatcacgc cctgagcggc atcttccacc
    46081 acaaatagat tatgcttctt cgccaacgcc ataatagtgt ccatttcgca ggccacaccc
    46141 gcgtaatgaa ccggcacgat aacgcgcgtt ttgtcggtga tcgccgcttc aatcagcgtt
    46201 tcgtcgatgt tcatggtgtc cgggcgaaca tccacaaaaa cgatttttgc gccacgcagc
    46261 acaaaggcat tggcggtgga gacaaaggtg tagctcggca tgatcacttc atcgccaggc
    46321 tggatatcga gcagcagcgc cgccatctcc agcgaagcgg tgcaggatgg cgtcagtaac
    46381 actttggcgc tgccaaaacg ttgctccagc cactgctggc agcgacgggt aaaaccacca
    46441 tcgccgcaca gtttgccgct acccattgcc gactgcatat agtcgagttc ggttcccacc
    46501 accggcggtg cgttaaatgg aatcatgtga tcacctgtat aaccagtacg tggtgctttc
    46561 tacattcgca ccactttgta tgtatcgttt aagcgcggcg gtgttgccca tctgggtcgc
    46621 cacccgcaaa gttgttttac cgcgagcata cgcccagttt agcgccgttt gcattagctc
    46681 agcacctgca ccgcgtccag ccagcaggcc aattcgcgca tctgtcgcat tgagttcccg
    46741 taaagagaca taaccgcgaa tatcgccgga cgccgcacgt aaaatcagac attgatgatc
    46801 aaaggtgccg cgcacggcat tttcaatcca ctgtgcataa aagcgaccgc tggcgtcagg
    46861 cgcataccac ggcgcacgaa aacggctttg cgcaaatgcg gcgctggcta actgacgtaa
    46921 tgcgggaata tcggtctctt gtgccactac tgcaccgcta tcactgacat tgttcacggg
    46981 tagtgccaaa tcaacttcac cttctaccag ggagaatccc agctgttgca gggcatccag
    47041 ttcacccgta tttgatgccg caattttggc ctgcacccgt gaccacggcg ctaacgcgtc
    47101 tggcgtcagg agcggtgctt cagacgtaat gcgcacgatg gcgctgttaa caccaaagaa
    47161 ggcgttttcc caggttagtg gctcaatact ggcgcggacg ggcacgaagt aactccagca
    47221 gatattggcc gtagccagtt ttcgctaatg aactggcagc acgcttcaca ccctcgtcat
    47281 cgagccagcc gttacgccag gcaatctctt ccaggcaggc aatcttaaag ccctggcgtt
    47341 tttccaccgt ctgtacaaag gtgctggctt caatcaggct gtcgtgagtg ccagtgtcca
    47401 gccaggcaaa tccgcgccca agcagttcaa cggtcaggtt gcccgcctcg aggtacatct
    47461 ggttgatgga ggtaatctcc agctcacccc gctccgacgg cttcacctgc tttgcgtact
    47521 ccacgacttt actgtcgtag aaataaagcc cggtcaccgc ccagtttgat ttcggctgtt
    47581 ttggtttttc ttccagcgag atagcgcgga aattgtcgtc aaactccacg acgccaaagc
    47641 gttccgggtc catcacctga tagccaaata ccgtcgcccc ttccgtgcgc gccgcaacat
    47701 gacgcagctt cggactgaag ccctgtccga agaaaatgtt atcgcccagc accagacaag
    47761 aaggttcacc gttgaggaaa gtttcaccga tgataaaggc ctgcgccaga ccgtccgggc
    47821 tgggctgttc ggcatattcc agctggatac cgaactcact accatcgccc agcaggcgct
    47881 ggaaataacc tttatcttcc ggcgtagtga tgatgagaat ttcgcggata ccggccaaca
    47941 tcagcaccga cagcggatag taaatcattg gcttatcgta aatcggcaac agttgcttcg
    48001 atacgccgcg cgtaatcgga tgcaatcggg tgccggaacc gcccgccagg ataatacctt
    48061 tcatgtttgc ctccgctgaa aattagcctt tcagacctaa acgctcgccc tgatagctgc
    48121 cgtcctgcac ctgcttccac cagctttcat tagccagata ccactgcacc gttttacgca
    48181 ttccactttc aaaggtttcc tgcggcagcc agccaagttc acgggcaatt ttcgaagcat
    48241 caatggcata acgcagatca tgccccggac ggtcagcgac aaaggtgatc aggtcacgat
    48301 agtgcgccac gccgtgcggc ttgttcggag ccagttcttc cagcagctcg caaatggttt
    48361 ccacaacatc gagattctta cgctcgttgt gaccaccaat attataggtt tcaccgactt
    48421 tcccggtggt cgccacgcaa tacagcgcgc gggcgtgatc ttcaacatac agccagtcac
    48481 ggatttgctg cccgttgcca tataccggca gcggtttacc cgccagcgcg ttgaggatca
    48541 tcagcgggat cagtttttcc ggaaagtggt aagggccgta gttattcgag cagttggtga
    48601 taagcgtcgg cagaccatag gtccgcagcc aggcgcgtac cagatggtca ctgctggctt
    48661 ttgaagcgga ataagggctg ctcggcgcat acggcgtggt ttcggtgaag aaatcatccg
    48721 tcgagtgcag gtcaccatat acttcgtcgg tggagatatg atgaaaacgg aacgctgatt
    48781 ttttatcttc cgtcagcgca ttccagtaag tccgcgccgc ttcaagcaat gtataagtcc
    48841 cgacaatgtt ggtttcaata aacgctgccg ggccgtcaat agaacggtca acatggcttt
    48901 cggctgccag atgcatgaca cagtctggct gatgctcagt gaatacgcgc gccagttctg
    48961 cccgatcgca gatatcaact ttctcaaagg caaagcgctc gctttgcgcg accggtgcca
    49021 gcgacatcag atttccggcg taggtcagct tatcgaccac taccaccgcg tcgctcgttt
    49081 cgttgatgat ataacgcacc agcgccgagc caataaaccc ggcaccacct gttatcagaa
    49141 tctttctcat cagcgccaaa ctcctttggc atcgacgaca tactgctgat ggacattgtc
    49201 gccattgata actttgaact gactatgatc gaccagcatc accagcacgt ctgccgttgc
    49261 cagcgcctcg tcaagctgcg ccagagtaca aagcccggtc agttttttcg gcaactggtg
    49321 gatgttaggc tcaacaacca gcgtttcgcc gctatgccac tgggcgagca gttcagcgat
    49381 ttccatcgcc gggctttcgc gcaggtcatc aatattcggt ttaaacgcca gaccaaagca
    49441 ggcgattttc agttcactgg cgcgtttatc ggtagcagca aggcagtcgg caaccgccgc
    49501 tttcacctga tcgataaccc agaacggttt gtgatcgttc acttcgcgcg cggtacggat
    49561 aagccgcgcc tgctgcgggt tctgtgccac gataaaccac ggatcaacag caatgcagtg
    49621 accgcccacg ccaggaccag gctgaagaat attaacgcga gggtgacgat tcgccaggcg
    49681 aatcagttcc cagacgttaa ttccctgatc ggcacaaatc agcgacagtt cgttagcaaa
    49741 agcgatattc acatcgcgga agctgttttc ggtgagctta cacatttccg ccgtccgcga
    49801 gttagtgacg acacactcac cttcgaggaa aattttgtac agttcgctgg cgcgggccga
    49861 acaaaccggc gtcataccac caatcacgcg atcgttttta atcagctcga ccataacctg
    49921 gcccggtaac acgcgttccg gacagtaagc aatgttgacg tccgccaact cgcccacctg
    49981 ctgtgggaaa gtgagatctg gacgcatctc tgctaaccat tctgccatct tctcggttga
    50041 ccccaccggc gaggtggatt caaggatcac cagcgcgcct tttttcagca ctggcgcaat
    50101 ggagcgagca gccgattcaa cgtaggtcat atctggctca tgatcgcctt taaacggcgt
    50161 gggtacagca atcagccagg catccgcttc aactggcgtc gtgctcgctc gtaaaaaacc
    50221 gccttctacg gcagttttta ctacgctcgc caaatcaggt tcgacgatat ggatttcgcc
    50281 acgattgatg gtatcaaccg cgtgttggtt gatatcgaca ccaatcacct gtttttgcct
    50341 tgaggcaaac gcagctgctg taggcagccc gatgtaaccc agtccgataa cagaaatggt
    50401 cgcaaaactc atagtgatat ccgattattt tttaacgctt ccagaatgcg agagcatgcc
    50461 tgaccatcac catacgggtt atgggcgcgg ctcatagctt gatattcgtt ttcgtctttt
    50521 aaaagacgcg tcacttcctc gacaattcgc tgcttatccg tgccgaccag acgcaccgtc
    50581 cccgctgtca ccgcttccgg acgctcagtg gtatcgcgca tcaccagcac cggtttcccc
    50641 agcgaaggcg cttcttcctg aatgccgcct gagtcggtca aaatcagcca ggcgtggttc
    50701 atcagccaga caaacggtaa atactcctgg ggatcgatca gaatgacatt tttcacatgc
    50761 cccagaatgc gattgaccgg ctctctgacg ttcgggttga gatgcaccgg atagacaatc
    50821 tggatgtcct ggtgcgtggt ggcgatgtct gccagcgcct ggcagatttc ttcaaagcca
    50881 cgaccgaagc tctcacgcct gtgaccggtc accagaatca tctttttatc ggggtcgata
    50941 aacgggtaat ttgccgccag ttctgaacgc agcgtgtcgc tgctcatcac ctggtcacgc
    51001 acccataaca gtgcatcgat gacagtatta ccggtaatga agattcggct gtcagcaacg
    51061 ttttcacgca gcaagttttg ccgggaagtt tcggttggag agaagtgata catcgccaga
    51121 tgcccggtca atgtacggtt agcctcttcc ggccacggcg aatagagatc gcccgtgcgc
    51181 agacctgcct caacgtgacc aactggaata cgctgataaa acgccgccag gctggttgcc
    51241 agcgtcgtcg tagtatcgcc gtgaaccagc acgacatctg gtttgaactc ggcaagaatt
    51301 ggttttagcc cttccagaat ccgacaggtt atctctgtca ggccctgtcc tggctgcatt
    51361 atgttgagat cgtagtcagg tacaatggaa aagagtttca gcacctgatc gagcatctcc
    51421 cgatgctgcg cagtgacgca aactttagcc tcaaaaaaag gatcttttgc caacgcatgc
    51481 accaacggag ccatcttgat ggcttccggg cgcgtaccaa atacagtcag tactttcaca
    51541 tcgattctct tcgaataagc ggcgagcgcc tttgcgctca ccacagcagt gttgctattt
    51601 cgagcaacgg cgggttaatg cgacaccagc cccgatcagt cccccgacaa tgccccacat
    51661 aatcatcagg aaggcacgac gtgggctatc gcgttttacc ggttcttccg gcgtacgcaa
    51721 atagcgatag gtctgaaaac gcggatccag ggttggacca acattcaggg tgtttaacat
    51781 ggcccgattc tgatcatagt cgagatcaaa ggccggaccg acggcctgta aattttccag
    51841 tcgagcctgg agcattggac gcccaagcag gaacatttct gaatcaggta gttcctcggc
    51901 aggcacatct gtcgcactgc gcgaaatatt atgctgctca gcaattttca gcgcctgttc
    51961 aatactgttc atccggcgat cgtagatagc tttcgccacc tcttcctgac gcttcacctg
    52021 agctttcatc tggatggtgc gtgccgccca tgcgcctttc agctcatcat tcagatggct
    52081 ggctgcacgc tggctggcaa aagcaacata ctgacgtaac aggttattag cgtcaggcgc
    52141 agtttcggca atcagcttca cgctgtcatt gaccgcgcgg gtgaagtcac cgggaataaa
    52201 cacgatgttg ttgatcattt catccagcaa cgccgcatcg gctttgctat tgcccaccat
    52261 ccgctgtttg taatagtcgg tttgcagcca gaactctctg cgggtatccc acgaggccag
    52321 ttgcataaca aactctttat aggcttcgtc catgaccgat ggttggtcgg cagaagccat
    52381 gtttgaacgg acatcgaggt tacgcaaaaa ttgctgctgc gagtaatatc cccccagcat
    52441 attcaccgtt ggacgatcgg taatcgccgt tgcgctccac tcctgtcgag caaaaaaagt
    52501 atacgccagc gcgattaacg caaacgccag ccccatgcca ataatccaca gcttcccagc
    52561 ccacaaggta cgaaacaacc cacgaatatc cagttcattt tcagcgtctt cggccggttt
    52621 cccaggcatt ggttgtgtca tcacatcctc atttatttgg ttaaattggg gctgccacca
    52681 cgatttctac gcagtctgcg ttttacgcgc ttaataaagc gagcaacttt ccaggcacgc
    52741 ttgatgcaat atccatagag gaagaatgct agcaaaaaga gcaccagcat gacccactcc
    52801 gggacaaaat gagaatattc tgccagcacg ccaatggaag cgagcagtgc tgcggcaagg
    52861 gtaatcagca caaacgcctg gcgggaagta aacccggcac gcatgatcaa atggtgaata
    52921 tgctgacggt caggagagaa tgggctcatg cctttacgca ggcgacggta cataatcgcc
    52981 accatatcca ttagtggaat ggcgattatc cataatgcag taaccgggct tattgggtgc
    53041 gtttttccct gagtcgtttc gagcaggatc cagataacgg taaaaccaat cagcgtactg
    53101 cccgcatccc ccataaagac tttgtagcgg cgacccagga taccaaggtt aagcatgatg
    53161 tatggcagga tagcggcgat catcgcaaag caccagattg cgagactggt ttgcccgtcg
    53221 aaccacaaaa tcataccgat tgctgcaaac gagacgcagg acaacccgcc cagcaagcca
    53281 tcaatgccat caaccatgtt gaacgcatta atggccgccc agacggcaaa tagcgtcagg
    53341 aagtaaccaa acggtccgag caccatctcc caggagccaa agatataacc caggctactg
    53401 agataaagtt tgccgaacac catcataaca atgccaacag cggcctgtat ggtggcacgg
    53461 atttttacgc tgatatcaaa acggtcatcc agcgcgccaa tgaaaacaag cacaccagca
    53521 caagcgagat agagagatgc atgcggaata tagtaatcga caattccgaa cgtgaagcaa
    53581 atccctgcgt aaaccgaaat ccccccaacg agaggtatca atccctggtg acgtttgcgg
    53641 aagtttggtt tatccactaa accgactttt tttgccacct tacgggcaaa aaacagaaac
    53701 agtgtcgtga ataaaaaaat actgatgaga tcagtactca ctgtcagtaa attcacaatg
    53761 catgctctca gagaaaatta ttagcagaag tataaccacg aagaccttta ttcagaaggg
    53821 aaagtccgtt ccgaacccac cagccctgtg caaaaattaa ccatttgata tgcaaattgc
    53881 ataacacacc agcaaattcc aggaattttc ctagcatcgc cagcgcggca ttaagattac
    53941 agatataaaa gcaaaacgcc acgtaaacac gtggcgtttt tggcataaaa caaatttatg
    54001 agcgtttcat catttcgaag aaatcgtcat tggtcttggt cattgccagt ttattaatga
    54061 ggaattccat tgcatcgatt tcgcccatcg ggtgaatgat tttgcgcagg atccacattt
    54121 tctgcagttc ttcctgagtc gtgagcagct cttctttacg ggtaccggaa cggttgtagt
    54181 cgatagccgg gaagacgcgt ttttcagcga tcttacgaga gaggtgcagt tccatgttgc
    54241 ctgtaccttt aaactcttcg tagataactt cgtccatctt ggaaccggta tcgataagcg
    54301 cggtagcgat gatggtcagg ctgccgccct cttccacgtt acgcgccgca ccaaagaagc
    54361 gtttcggacg atgcagagca ttggcatcca caccaccggt caacacttta cctgacgccg
    54421 gaacaacggt gttgtaagcg cgcgccagac gagtgatgga gtcgagcaga atgataacgt
    54481 ctttcttgtg ctcaaccagg cgtttggcct tctcgatcac catttccgca acctgaacgt
    54541 ggcgagatgc gggttcgtca aaggtagaag caacaacttc acctttaacc agacgctgca
    54601 tctcggtgac ttcttccgga cgttcgtcga tcagcagaac catcagtacg caatccgggt
    54661 ggttgtaagc aatgctctga gcaatgttct gcagcagcat ggttttaccg gctttcggcg
    54721 gtgccacaat cagaccacgc tgaccacgac cgataggtga tgccagatcc agtacgcgag
    54781 cggttaaatc ttccgtagaa ccgttaccac gttccatacg cagacgagag tttgcgtgca
    54841 gcggggttaa gttctcaaag aggattttgt tgcgggcgtt ttcaggtttg tcgaagttaa
    54901 cttcgttaac tttcagcagc gcaaaatagc gttcaccttc tttcggcggg cgaatcttac
    54961 cagagatggt atcaccagtg cggaggttga aacggcggat ttggctaggg gaaacgtaga
    55021 tgtcatcagg accggcgagg taggagctgt ctgcggaacg gaggaaacca aatccatcct
    55081 gcaatatctc cagtacgcca tcaccaaaga tatcttcgcc actctttgcg tgctgcttca
    55141 ggatggcaaa aataatgtcc tgcttacgca tacgagccag gttttccagc cccatatttt
    55201 cgccgagagt gatcagctca gaaaccggcg tattctttaa ttcggtaaga ttcatagtgg
    55261 tgtgagttct taaacttggg gtaaatctcg aacttaatgt tgtgaatggt atggcaggat
    55321 catccatgcc tgtttacggt catcgactca tgtcttttcg ctgtctggtc acaggagagt
    55381 acgcagaact gaaacgacaa gacggattga gtgacgagcc agaaatctgt tcacttcgca
    55441 tttaagataa aacgggaagc gttggaaatt acaagattca aacttaataa ggtatgttta
    55501 atacgaagtc aacactaagt tagcatgact cacgccgggc gtccagtttt tagcgacggg
    55561 gcacccgaac atgaaattcc cttacgccag gttagcgtcg aggaactctt tcaactgacc
    55621 tttagacagt gcgcccactt tggttgccgc cacttcaccg tttttgaaca gcagcagagt
    55681 cgggatacca cggatgccat atttcggcgc agtgccaggg ttttgatcga tgttcagttt
    55741 tgcaacggtc agtttgccct gatattcgtc agcgatttca tccagaatcg gggcgatcat
    55801 tttgcacgga ccgcaccact ctgcccagaa atcaacgagg atcgccccgt ccgctttgag
    55861 tacatccgtg tcaaaactgt cgtcagtcag gtgaataatt ttatcgctca tatataactc
    55921 cacaggaata agcctggcgt gttggtttcg ttgttggtgt aacattaacc aactaaaggt
    55981 tgactttatt tcaccggata cgctttcgta aagcaatagt aagctgatat tctaccacac
    56041 tatgagcaaa acacatttaa cagaacagaa gttttccgac ttcgccctgc atccgaaggt
    56101 tgtagaagcc cttgaaaaaa aagggtttca taactgtacg cccattcagg cactggccct
    56161 tccgctgacg ctggcgggtc gtgacgtagc cgggcaggcg caaaccggta ccgggaaaac
    56221 gatggcgttt cttacgtcaa cgtttcatta tcttctctct catcctgcga tagccgatcg
    56281 caaggtgaat caaccgcgtg ccttaattat ggcaccgacg cgtgaacttg ccgtgcagat
    56341 ccacgccgac gcggagccac ttgcgcaagc taccggcctg aagctgggtc tggcttacgg
    56401 tggtgatggc tacgacaaac agctgaaagt gctggaaagc ggcgttgaca ttctgattgg
    56461 caccacgggg cgtttaattg actacgccaa gcagaaccac attaacctcg gtgccattca
    56521 ggtagtggta ctggacgaag ccgatcgtat gtacgatctg ggctttatta aagatatccg
    56581 ctggctgttc cgccgtatgc cgcctgcaaa ccagcgcctc aacatgctgt tctccgccac
    56641 gctttcgtac cgggtacgtg aactagcgtt cgagcagatg aacaatgccg aatatattga
    56701 agtggaaccg gaacagaaaa cgggccaccg tattaaagaa gagcttttct acccttctaa
    56761 cgaagaaaaa atgcgtttgc tgcaaacgct gatcgaagaa gagtggccag acagagcgat
    56821 tattttcgcc aacaccaaac accgttgtga agagatctgg ggccacctgg cggcagatgg
    56881 tcatcgtgtc ggtttgttga caggcgatgt cgcgcagaaa aaacgtctgc gtattcttga
    56941 tgaatttacc cgtggggatc tggatattct ggttgccacc gacgttgccg cgcgtggttt
    57001 gcatattccg gcagtgacgc acgtctttaa ctacgattta cccgatgact gtgaagatta
    57061 cgttcaccgt attggtcgta caggtcgcgc aggcgcaagc ggtcactcta tcagcctggc
    57121 ctgtgaagag tatgcattga atttgcctgc tattgagacc tatattggtc actcaattcc
    57181 ggtaagtaaa tacaatccgg acgcattgat gaccgatctg ccaaaaccgc tgcgcctcac
    57241 gcgcccgcgc acaggcaatg gtccgcgtcg tactggcgct ccgcgtaatc gtcgtcgttc
    57301 aggttaaata aaaaatgtca tactccaaat cattcaagct gtatcaagat ggcaagagaa
    57361 tgaatcccga tgagtttact gtagtaagtg attcaggtga aagaatgcag ccaacacaga
    57421 gacagattga aggatgaaga gtatgggttc cacctcgtcg ctgtatgcag ccattgatct
    57481 cggttcgaat agttttcata tgctggttgt gcgcgaggtg gctggaagca tccagacgct
    57541 gacgcgaatt aaacgcaaag tgcgtctggc tgctggcctg aatagcgaaa atgccctgtc
    57601 taatgaagca atggagcgcg gttggcaatg tctgcgcctg tttgctgaac gtctgcaaga
    57661 tatccctccc tcgcaaattc gcgttgtcgc tacggcgaca ttacgactag ccgtcaatgc
    57721 gggtgatttt atcgccaaag cacaggaaat cctcggttgt ccggtacagg tgatcagcgg
    57781 tgaagaggaa gcacgtctga tttatcaggg cgttgctcac acaactggcg gtgccgatca
    57841 gcgcctggtg gtggatatag gcggtgccag tacggaactg gtaaccggca cgggtgcaca
    57901 aaccacctcg ttgttcagcc tgtcgatggg ctgcgtcacc tggctggaac gctattttgc
    57961 cgatcgtaat ctggggcagg aaaattttga tgctgcagaa aaagcggcac gcgaagtgtt
    58021 acgtccggtt gccgatgaat tacggtatca cggctggaaa gtgtgcgttg gcgcttccgg
    58081 caccgtgcag gcgttacagg aaatcatgat ggcacagggg atggatgaac gcattaccct
    58141 ggaaaagttg cagcaactga aacaacgcgc cattcattgc ggtcggctgg aagagctaga
    58201 gattgacggg ctgacgctgg aacgtgcgtt agtgttcccg agtggtctgg cgatcctgat
    58261 agccattttt accgaactga atattcagtg tatgaccctg gcgggcggtg cgctgcgtga
    58321 aggcctggtc tacggtatgt tacatcttac cgtcgagcag gatattcgca gccgtacgct
    58381 gcgtaatatt cagcgccgct ttatgatcga tattgatcag gcacagcgcg tagccaaagt
    58441 tgcggctaac ttcttcgatc aggtggaaaa tgaatggcat cttgaagcaa taagccgcga
    58501 tttgctcatc agcgcctgcc agcttcatga aatcggcctg agcgttgact tcaaacaagc
    58561 gccgcaacac gctgcttatc tggtacgtaa tctggatctt cccggtttta cccccgcaca
    58621 gaaaaaactg ctggcgacgc tactgctcaa ccagactaat ccggtcgatc tctcatcgct
    58681 gcatcagcaa aatgccgtac caccgcgcgt cgcagaacaa ctctgccgtt tactccgcct
    58741 ggctatcatt tttgccagcc gtcgccgtga cgatctcgtg ccagagatga cattacaggc
    58801 taaccatgaa ctgttgacct tgacgcttcc gcaaggttgg ctaacccaac atccgctggg
    58861 taaagagatt attgatcagg aaagccagtg gcagagctat gtccactggc cgctggaagt
    58921 gcattaatga gtaagtgccg gatgcgatgc tgacacatct tttccggcct tgattatttc
    58981 cctcgttttg ccgccatcat cgctttcaga ttcgccagat ggctttgccc tttctgcatc
    59041 cgttcttcgg cgctgaccac tttgcgctcc tgttcccaaa tcagatcatc ctgcggcagc
    59101 tccagcaaaa agcggctcgg ctccgggcgc accagttcgc cgtactgacg gcgttcttta
    59161 cacagcgtaa aggtcaattc cttctgggcg cgggtaatgc cgacataggc cagccgccgc
    59221 tcctcatcga tattatcttc atcgatgctg ctctggtgcg gcaaaaaccc ttcttccata
    59281 ccgaccatgt agacataagg aaactccagc cctttcgacg cgtggagagt catcagttgc
    59341 acctgatcca gctcttcttc actctcacca cgctccatca tgtcgcgcaa agtaaagcgc
    59401 gtcaccacct gggttaacgt catcggctca tccagttcac tgccttcgag catctccgtc
    59461 atccagctaa acagttggtt gacgttcttc atgcgcattt cggcggcttt cgggctgggc
    59521 gatgtttcgt acagccagga ttcataatcc atgccgtgaa tcagatcacg caccgcggca
    59581 atcggctctc gctccgccag gcgctggatt tctgccaacc agtgagtgaa gcgggtcaat
    59641 gcttcataac cacgtccgct aagcgtctga ctcaggccca tatcaaagct ggcggtaaac
    59701 atgcttttat tgcgcgtcat cgcccactca cccagctttt tcagcgtagc cgggccaatc
    59761 tctcgcttcg gcgtgttaac gatacgcagg aatgcgctgt catcgtccgg gttagtcagc
    59821 acgcgcagat aagccagcaa atccttgatt tcagggcgag agaaaaacga cgtaccacca
    59881 gaaattttgt acgggatgcg gttttgcatc aggaattttt caaacacccg cgactgatgg
    59941 ttgccgcgat aaagaatggc gtaatctttg tactgcgttt tattgacgaa gtgatgcgcg
    60001 atcagctcgc cggtgactct ctcggcttca tgttcttcgt tattcgcgct taaaactttt
    60061 agctccgcgc catagcccag ttcggagaac agacgctttt caaagacgtg cgggttattg
    60121 gcgatcagga tgttcgccgc tttcagaata cgtccggaag agcggtagtt ctgctcaagc
    60181 ttaatcacct tcagtgccgg gaaatcctga ctcaacagca ccaggttttg cggacgtgca
    60241 ccgcgccagg agtagatcga ctgatcgtca tcgccaacta cggtaaagcg cgcgcggctg
    60301 cccaccagca gtttcaccag ttcatactgg ctggtgttgg tgtcctgata ctcatccacc
    60361 agcaggtagc gaattttgtt ctgccagcgc tcgcggactt cttcattgcg ttgcagcagt
    60421 aacgtcggca ataaaatcag atcatcgaag tcgagaacgt tacaggcttt caggtgtgca
    60481 tcatacagcc cataacaatg ggcaaaaata cggtcccgct cgccaatcgc actggctgcc
    60541 gcctgggacg gtgttttgag atcgttcttc cagttagaga tggtcgaaat cagctgttgc
    60601 aggaggactt tgtcatcttc aatcagcccc tcggtcagct ctttaagcaa cgccagctga
    60661 tcggtgtcat caaacagcga aaagttcgct ttcatcccca acgccgcata ctcacgcttg
    60721 atgatatcca gccccagcgt gtggaaggtg gaaatcatca gcccgcgcgc ctctttgcgc
    60781 cccagcgtct gcccgacacg ctctttcatc tcgcgcgccg ccttattggt aaaggtcacc
    60841 gccgcgatat gtcgcgcctg ataaccgcaa ccgcggatca gatgggcgat tttattggtg
    60901 atgacacgag ttttaccgga acccgcgcct gccagcacca ggcaggggcc ggtaacgaat
    60961 tcgacagctt gttgttggcc ggggtttaga cgcataggta tattgctcaa tcttcgaacg
    61021 ggggggagga ttgtagcaga atcgcatttg aatgtgaccg agtgaaaacc gctaaccgga
    61081 tggctgcgga tgtttaaatc agagagggaa ggccgaaacc ggagttcatc atgaatccat
    61141 tagacttaga aaatatcggg tcgtaacgtt gtgttattac tttttcagag tttactaagg
    61201 agaggtttac tcgtggcgat taatttcagc cccaaggttg gtgaaatact ggaatgcaac
    61261 tttggtaact atcctgttag ccagaatgga cagttcagca cgacgtatta cgatggccgc
    61321 attccacctg agatgataaa aaataggttg gttgtcgtgc tcaacggaaa aattaatggc
    61381 aacgcatgta tcgtcgttcc attatcaacc actcgcgacc atgacaagtt aaatcgtgga
    61441 atgcatgttg aaatagccag taatgttatc aatgacctgc aattctttga tcagcagata
    61501 cgttgggcta aagccgacct ggtacaacaa gtcagtcgaa atcgattaaa cagagcaagg
    61561 acttatcggg ggtatcttaa tcaatgtttg ccacatgagt tggttgcaga catacagagg
    61621 gcagtcatca agtcaatcaa tgccatatcg ctcataaatt gacaataaaa tcaccatccc
    61681 cgataatgac tctgtagccc atagaggccc taagttttct ccccttgagg gacctgcgaa
    61741 gcccggattt tccgggcttt tgccatatat gtccccccct ccctgactga caatttttaa
    61801 ttcaaggcac gatcatggca aaaacagcag cagcactgca tatccttgta aaagaagaga
    61861 aactggctct ggaacttctc gagcagatta agaacggtgc cgatttcggc aagctggcga
    61921 agaaacactc catttgcccg tcaggtaaac gcggtggtga tttaggtgaa ttccgccagg
    61981 gccagatggt tccggcgttc gataaagtgg ttttctcttg tccggtactg gagccaaccg
    62041 gtccgctgca tacccagttc gggtatcaca tcattaaggt gctttaccgt aactaacagc
    62101 aaggccttct ccgggagaag gccttgaact ctgaatcgca cttaacctgc aacagcaata
    62161 cgtttcatat ccgtcatata gccgcgcagt ttcttaccta cctgctcaat cgcatggctg
    62221 cgaatcgctt cgttcacatc acgcaactgc gcgttatcta ccgcaccttc cggaatagct
    62281 ttacccaagt cgcccggttg cagctctgcc ataaacggtt tcagcagcgg cacacaagcg
    62341 taagagaaca gatagttacc gtactcggcg gtatcagaga taaccacgtt catttcgtac
    62401 agacgcttac gagcgatagt gttggcaatc agcggcagtt cgtgcagtga ttcatagtaa
    62461 gcagactctt cgatgatgcc ggaatcaacc atggtttcaa acgccaactc aacgcctgct
    62521 tttaccatcg cgatcatcag tacgccttta tcgaagtact cctgctcgcc gattttgcct
    62581 tcatactgcg gcgcggtttc gaacgcggtt ttgccggtct cttcacgcca ggtcagcagt
    62641 ttcttatcgt cgttggccca gtctgccatc atgccggaag agaattcgcc ggagatgatg
    62701 tcatccatgt gtttctggaa cagcggcgcc atgatctctt tcagctgttc agacagcgcg
    62761 taagcacgca gtttcgccgg gttagagaga cggtccatca tcagcgtgat gccgccctgt
    62821 ttcagcgctt cggtgatggt ttcccagccg aactgaatca gtttttctgc gtatgccgga
    62881 tcggtgcctt cttccaccag tttgtcgaag cacagcagag agccagcctg caacataccg
    62941 cacaggatgg tttgctcgcc catcaggtca gatttcactt ccgcaacgaa agaggattcc
    63001 agcacgcccg cacgatgacc accggttgcc gccgcccacg ctttagcgat cgccatgcct
    63061 tcgcctttcg gatcgttttc cgggtgaacg gcaatcagcg tcggtacgcc gaatccacgt
    63121 ttgtactctt cacgcacttc ggtgccaggg catttcggcg caaccattac tacggtgatg
    63181 tctttacgga tctgctcgcc tacttccacg atgttgaaac catgcgagta acccagcgcc
    63241 gcgccgtcct tcatcagtgg ctgtacggtg cgcactacat cagagtgctg cttgtccggc
    63301 gtcaggttaa ccaccagatc cgcctgtggg atcagttctt cgtaagtacc cactttaaaa
    63361 ccattttcgg ttgctttacg ccaggatgcg cgcttttcgg caatcgcttc tttacgcaga
    63421 gcgtaggaga tatcgagacc agaatcacgc atgttcaggc cctggttcag accctgtgcg
    63481 ccacagccga cgatgactac ttttttaccc tgaaggtagc tcgcgccatc ggcgaattca
    63541 tcgcgcccca taaagcgaca tttgcccagc tgtgccagct gctggcgcag attcagtgta
    63601 ttgaagtagt tagccatggt gattcctcgt gatgttgtgc ttcttattgt tcggttcgcg
    63661 tttgcgagat tgaattcact atatgacagg aaatttattg cggaaattga tatattcaca
    63721 acgtcacatt gcaatttttg caacgtcaac attgagggct gcgccattgg atttacgcga
    63781 tctgaaaacc ttcctgcatc tggcggaaag ccgccatttt gggcgcagcg cgcgggcaat
    63841 gcacgttagc ccgtctacgc tttcgcgaca aatacagcgc ctggaagaag atctcggtca
    63901 gccgctgttt gtgcgtgata accgcacggt aacgctgacg gaagcgggcg aagaactgcg
    63961 cgttttcgcc cagcaaacgc tgttgcaata tcagcaattg cgccacacca tcgaccagca
    64021 agggccgtcg ctctctggtg aattacatat cttctgctcg gtgaccgctg cctacagcca
    64081 tcttccaccg attctcgacc gcttccgcgc ggaacatccg tcagtggaga tcaaactcac
    64141 taccggtgat gcagccgatg cgatggaaaa ggtggtcact ggcgaagcgg atctggcgat
    64201 tgccgggaaa ccggaaacct tgccgggcgc agtggcgttt tcgatgctgg agaatctggc
    64261 ggtggtgctg attgctcccg cgctgccctg cccggtgcgt aatcaggtct ctgtcgaaaa
    64321 gcccgactgg tctacagtgc cgtttatcat ggccgatcag ggaccggtac gccgccgcat
    64381 tgaactgtgg tttcggcgta acaaaatcag caacccaatg atttacgcca cggttggcgg
    64441 gcatgaagcg atggtatcga tggtggcgct cggctgtggc gtggcgctgt tgccggaagt
    64501 ggtgctggat aacagcccgg aaccggtgcg taaccgcgtg atgattttag agcgcagtga
    64561 tgaaaaaacg ccgtttgagc tgggcgtgtg cgtacaaaaa aagcggcttc atgagccgct
    64621 gattgaggcg ttctggaaga ttttgccgaa ccactaaccc gccaaaaaga acctgaacgc
    64681 cgggttattg gtttcgtcgt ggcaatcgta acccagttca ttcagccggg tttcaaaatc
    64741 cggttcatga tcgccaagct cgaacgccgc cagtacgcgc ccatagtcgg taccgtggct
    64801 gcgataatgg aacaaagaaa tgttccagtg cgtacccagc gtgttgagga agcgcagcag
    64861 cgcgcccggt gattccggga attcgaaact gtagaggcgt tcctgcaacg gatgcgatgg
    64921 acgcccgcca accatatagc gcacatgcag cttcgccatt tcgtcgtcgg agagatcaac
    64981 cacgctgtag ccaccgtcgt tgagcatctg caaaatttct ttgcgctctt cgaggccacg
    65041 gcttaagcgc acgccgacaa agatgcaggc gtttttggca tcggcaaaac ggtagttgaa
    65101 ctcggtgacc gaacgcccgc caagcagttg gcagaatttg aggaagctgc ctttttcttc
    65161 cggaatggtc accgccagta acgcttcacg ctgttcgccc agttcgcagc gttccgagac
    65221 gtagcgcaga ccgtgaaagt tcacgttagc accggaaaga atatgcgcca gccgctcacc
    65281 gcgaatgttg tgctgggcga tatatttttt cattcccgcc agtgccagcg cgccagaggg
    65341 ttccgccacc gcgcgcacat cttcgaacag atctttcatc gccgcacaga tggcatcgct
    65401 atcgacggtg atgatgtcgt cgagatactc ctggcataaa cggaaggttt cgtcgccgat
    65461 gcgtttcacc gcaacacctt cggcaaacag ccccacgcgt ggcagatcaa ccggatgacc
    65521 tgcattcagc gccgctttca ggcaggcgga atcttccgct tccacggcga tcactttgat
    65581 ttgcggcatc agttgtttga tcagtaccgc gacgcccgcc gccagaccgc cgccgccgac
    65641 cggtacaaat acgcggtcga gatgggcgtc ctgctggagc agttccagcg ccagcgtgcc
    65701 ttgcccggcg atcaccatcg gatggtcgaa cggcggcacc caggtgaagc cctgctgctg
    65761 tgacagttcg attgctttcg ctttcgcttc gtcaaagttc gcgccatgaa gcagcacttc
    65821 gccgccaaag ccgcgcaccg catcaacttt gatatcggcg gtggcggttg gcatgacgat
    65881 cagcgccttc acgcctaacc gtgcggaaga aaacgcgacg ccctgcgcgt ggttacccgc
    65941 agaagcggtg atcacgccgt gcgctttttg ttcttccgtc aggcccgcca tcatggcgta
    66001 tgcgccgcgc agcttaaagc tgtgtactgg ctggcgatct tcgcgcttca ccagaatcac
    66061 gttatcaaga cgcgacgaca gtttttccat tttctgtagc ggcgtgacct gcgccgcttc
    66121 gtagaccggc gcgcgcagca ccgctcttaa atattcggca ccttcagggg caccggacag
    66181 gggttgtgag tctgccatca ttaacccccc agtttcgatt tatcgcgcac cgcacctttg
    66241 tcggcgctgg ttgccaggct ggcgtaggca cgcagcgcaa aggaaacctg gcgttcacgg
    66301 tttttcggcg tccaggcttt gtcgccacga gcttcctgcg cttcacgacg cgccgccagt
    66361 tcggcatcgc ttacctgtaa ctgaatacca cggttcggaa tgtcgatggc gatcaggtcg
    66421 ccgtcttcaa tcaggccaat gctgccgccg cttgccgctt ccggtgagac gtggccgata
    66481 gaaaggccag aggtgccgcc ggagaagcgt ccgtcggtga tcagcgcgca ggctttaccg
    66541 agacccattg atttcaggaa gctggttggg tagagcattt cctgcatccc cggaccgcct
    66601 ttcgggcctt catagcggat caccactaca tcgccagcga caactttgcc gccgagaatc
    66661 gcttctaccg cgtcatcctg gctttcgtac actttcgccg ggccggtgaa tttgaggatg
    66721 ctgtcatcga cgcccgcggt tttaacgatg cagccgtttt ctgcgaaatt accgtacagc
    66781 accgccagac caccttcttt gctgtaggcg tgttccagcg agcggataca gccattggcg
    66841 cgatcgtcat cgagagaatc ccaacggcaa tcctgcgaga atgcctgtgt agtacgaatg
    66901 cccgcagggc cagcgcggaa catatttttt accgagtcat cctgggtcag cataacgtcg
    66961 tattgttcca gcgtttgtgg caacgtcagg ccaagcacgt ttttcacatc acggttcagt
    67021 aaccccgcgc gatccagttc gccgagaata ccgataacac caccagcacg atgaacatct
    67081 tccatatggt atttctgggt gctcggcgca actttgcata actgcgggac tttacgcgaa
    67141 agtttgtcga tatcactcat ggtgaagtcg atttccgctt cctgcgccgc cgccagcagg
    67201 tgaagtacag tgttagtcga tccacccatc gcgatatcca gcgtcatggc gttttcaaac
    67261 gccgccttac tggcgatatt acgcggcagt gcactttcgt cattttgctc gtagtaacgt
    67321 ttggtcaatt caacaatgcg tttaccggca ttgaggaaca gctgcttacg gtcggcgtga
    67381 gttgccagca gcgagccgtt gcccggctgc gacaggccca gcgcttcggt caggcagttc
    67441 attgagttag cggtaaacat cccggagcag gaaccgcagg ttgggcatgc ggaacgttca
    67501 acctgatcgc tttgggagtc agaaactttc gggtctgccc cctggatcat cgcatcaacc
    67561 agatcgagct tgatgatctg atcggacagt ttggttttcc cggcctccat cgggccgccg
    67621 gaaacaaaga tcaccggaat attcaggcgc agggaagcca tcagcatccc cggggtgatt
    67681 ttgtcgcagt tagagatgca gaccatggcg tcggcgcagt gagcattgac catatactca
    67741 acggaatcag cgatcagttc gcgagatggc agcgaataaa gcatcccccc gtggcccatg
    67801 gcaatcccat catccaccgc aatggtgttg aactctttgg caacgccgcc agccgcttca
    67861 atttgttcgg cgaccagttt accgagatcg cgcagatgga cgtgacccgg tacaaattgg
    67921 gtgaacgagt tcacaaccgc gataatcggc ttaccgaaat cggcgtcggt cattccggtg
    67981 gcgcgccaca gcgcacgagc acccgccata ttacgaccat gagtggtggt ggcggaacgg
    68041 tacttaggca tactttattt actcccagtg tctgtctcgt aaatgggacg gtgcgtgccg
    68101 tcccattttt tgtatttatt gattaacttg atctaaccag ccccatttat cttcggtttc
    68161 gccagtgaag aggccgaaga aggcttgctg aatgcgtttg gtaaccgggc cacaacggcc
    68221 ttcgccaacc tgaataccgt ctacgctgcg cactggcgtg atttctgccg ccgtaccgga
    68281 cataaacact tcatccgcca ggtacaggga ttcgcgcgac agcacctgct cacgcacttc
    68341 aattcccagc tcttttgcca gtttgatgat ggcatcacgg gtaatacccg gcagcgcgga
    68401 tgaggtgaac ggtggggtga acagcacgcc gtctttcact tcaaacaggt tttcgcctgc
    68461 gccttcagag atgtaaccat tcacatccaa cgcgatacct tcctgataac cgtggcggcg
    68521 cgcttcgcta cccaccagca gggaagagag gtagttacca ccggcttttg ccgccgtcgg
    68581 gatggtgttt ggtgctgcgc ggttccagga ggaaaccatc gcatcgatcc cctgctccag
    68641 tgcttctgcg cccagatacg ccccccacgg gaaagcggcg ataatcacat cggttgagta
    68701 tcccgctggc gggtttacgc ccatgccaac atcaccgacg aaaatcagcg gacggatata
    68761 ggcgctggtg agattgtttt tgcggatcac gtcgcgacaa gcttccatca gctcatcaat
    68821 gctctgtgaa accgggaagc gatagatttt ggcggagtca tgcagacgct gcatatgctc
    68881 acgatggcgg aatacaaccg gtcctttgtg cgagtcgtag caacggatgc cttcaaaaac
    68941 cgaggtgcca tagtgcagcg cgtgcgacat cacatgcacc ttcgcgtctt cccagcgaac
    69001 catctcccca ttgaaccaaa tgtaatcagc tttcttcgtg gtcattttta tattcctttt
    69061 gcgctcaggc gcggatttgt tgtgatgtgg ttgtgctctg gcagatggca acgtgtgcga
    69121 cgtccaccag tttatttaac tgactaaaca gtaagtcgac cgaccgtggg ctggcaacgg
    69181 tcaattcgat atttatattt tgtgcatcgc tggcggcggc catattcatt gagcagacgt
    69241 ggaaaccacg atgacgcacc acgcgtaaaa cacgttctaa ggtttccgga ttgaagcgag
    69301 ccgatacatt gacctgatgt tgcatcatga taatttctcc aacatttctg aattactggc
    69361 acctggcggc accagcggcc agacgttctc aagttcgtcg attgagacat gaagcaggta
    69421 tggcccatca ctgttcagca tggtgtcgag tgccgcttca acctggtctt tacgggtgat
    69481 gtgttggcca gggatgccga aggcgctggc taacatgagg aaatcgggat tatcggtaag
    69541 ggtggtttcg ctatatcgtt cctggaaaaa cagttgctgc cattgtcgaa ccatccctaa
    69601 ccgttggtta tcgagtaaga cgattttcaa cggtaactgc ttgcgtttta cggtgcccag
    69661 ctcttgcaca ttcatcatga aagagccgtc accggagata cagacgacgg tatcgttcgg
    69721 tcgcgcgact tgtgcgccaa ccgccgccgg taaaccaaaa cccatggtgc ctaagccgct
    69781 ggaggtgatg aaattttccg ggcgagtgtg gacgatgtgc tgggcagacc acatctggtg
    69841 ctgccccaca tctgtggtca cgatgcaatc cgcaggttta cgatccgaca gttgttttaa
    69901 taacaacggc gcgtagatag cgtcaccagg atggtcgtaa cgccaggcat gttcatcacg
    69961 cagttgtgcg cagtgttgct gccagtcatc gatatttaac ggctgctgta atgctggtaa
    70021 cagagcattt aaatcaccct gtaatgccgc atgtgcctga cgcagcttgt tcatttctgc
    70081 cggatcgata tccatatgga taacgctggc gtgtggtgcg aaggtgttca gtttgccggt
    70141 cacccggtca tcaaaacgtg cgcccacggc tatcagtaga tcgcactcct gaaccgcgaa
    70201 gttcgccgct ttggtgccgt gcattcccag catgcccaga tagtacggat aatctgcttc
    70261 aactgcgccc agccctttca gcgtgcaggt ggcaggcatt tttgtggtag cgagaaattc
    70321 acgcaaagcc ggaactgcct gcgccatgcc aacgccaccg ccaacgtaca gcatcggttt
    70381 ttgcgctttt gccagcatct ggcgcgcttg ctcgacttcg gcatggggga aagtcacttc
    70441 gttttcaacg gtggtgaacc acggttccag atcgccgctg gctaactgga tatcttttgg
    70501 aatatcgacc aaaaccggac caggacgacc agagctggcg acgtcaaacg cttcggccat
    70561 gatacgcggt aactcttcca gtgattgcac caggaaactg tgcttggtgc aggctaacga
    70621 caaccccaga atatccacct cctgaaatgc gtcagtgccg ataaacggtg cggatacctg
    70681 accggtgata gcaacaacgg ggatggaatc taacagtgcg tccgcaagcc cggttatcag
    70741 gttggttgcg cccggaccag acgtggcgat acatacgcca gttttgccag tagcacgggc
    70801 ataaccgata gccgccattg ccgcaccctg ttcatgtcgg cacagcaagt gctccatgcc
    70861 gccgtcatac aatgcatcgt aaaccggcat aattgcgcca cccggataac cgaaaacggt
    70921 attcacaccc tgtgcccgca acgcatgtac cacccactgt gcgccattca tagttagttc
    70981 cccgtcctga atcttgagaa acagaatttt gtgctgttat tcattgtctg ctcctcggtt
    71041 atgtttttaa ggtcaaaaaa aacccccgga cctttcggtg cgggggtctt agttcgttaa
    71101 ggcttgatct ctaagccttt cctcgtccaa gtgcagcccc gcacggtggg ataataatca
    71161 ccaccacgct aatcacgacc aggctaatca ctcgtagaag ggctgtcatt ttgtcttttc
    71221 ttgaatcttg ttcgaaggaa tgcctaaaga gttaccatag attttgcgaa tagcacaaga
    71281 tattttttgg ccgattttta tgatgtcttt aataatttca agagaaattg ttgtttttta
    71341 aaaagaaaat agtgagtgaa aaaaattcaa tttcagtatg aatgttgttc ccttcctcgt
    71401 agttcatcct ttgcccgctg catttttgcg agcatcattc caccgccaga acaactcaac
    71461 ttcacttgca caaataaaca ggaatcagcc actaaaattc acgaattagc gggctacagc
    71521 agcgatatct tatgcacata atggtgactc aaggagggct tatgtcactg tcaattgttc
    71581 atacccgcgc agccctggga gtaaatgcgc ccccaatcac tgttgaggta catatcagta
    71641 aaggtctacc cggcttaacg atggtgggct taccagaaac aacggtaaaa gaagcccgcg
    71701 atcgcgtgcg cagcgccatt atcaatagcg gatatgaata tccggcgaaa aaaatcacca
    71761 tcaaccttgc tccagccgat ctgccaaaag aagggggacg atatgattta cctatcgcca
    71821 ttgcgttgct ggcagcctca gaacagctta cagccaataa gttagatgaa tatgaattag
    71881 tcggagaact ggcgcttaca ggcgctctgc gtggcgttcc cggcgcaatc tccagtgcaa
    71941 ctgaagccat taagtcgggc agaaaaatta tcgtcgcgaa agataacgag gatgaagtgg
    72001 ggctaattaa cggtgaagga tgcctgatag ccgatcatct gcaggctgtc tgtgcgtttc
    72061 tggagggtaa gcacgctctc gaacgcccga aaccaactga tgcagtatcc cgggcgctac
    72121 aacatgatct cagtgatgtt gtcggtcagg agcaaggaaa gcgaggactg gaaattaccg
    72181 ccgctggcgg gcacaacctt ttactgattg gaccgccggg aacaggtaaa acaatgctcg
    72241 ccagccgtat taatggcctt ttgccagatt taagcaatga agaggcactg gagagcgctg
    72301 cgatattaag tctggtaaat gctgaatcag tacaaaaaca atggcggcag cgcccgttcc
    72361 gctcacctca tcacagtgcg tcgttaactg cgatggtggg cggtggtgca attccagggc
    72421 caggtgaaat ttcgctggcg cataacggcg tgctttttct tgatgagcta cctgaatttg
    72481 aacggcgtac actggatgcc ttgcgagagc cgattgaatc cgggcagatc catctttcac
    72541 gcacacgagc aaaaataacc tatccagccc gtttccagct tgtcgcggcg atgaatccca
    72601 gccctaccgg acattatcag ggaaaccata accgctgcac accagaacag acattacgtt
    72661 atctcaaccg gctctcgggg ccctttctcg accgcttcga tctctcacta gagatcccat
    72721 taccgccccc cggcattttg agtaaaacgg tagtgcaggg agaaaacagc accaccgtta
    72781 aacaacgtgt aatggccgcc agagagcgcc aatttaagcg gcagaataaa ctgaacgcct
    72841 ggctggataa tccggaaata cgccaattct gcaagcttga gagcgaagat gcgcagtggc
    72901 tggaagaaac gctgatccat ctggggttat cgattcgtgc ctggcagcgg ttattgaaag
    72961 ttgcacgaac cattgctgat attgatcagt ctgacattat cacacgtcag catttgcagg
    73021 aggcagttag ctatcgtgcg attgaccgtt tgctcatcca tctgcaaaaa ctactgacat
    73081 aaaaaaaggg catttcgccc tttttattaa tcgtcagaat cggtgtagtc ttcagcacct
    73141 tcaacctgcg gtttaccgcc ggaaagggtg tgaaaacgtt ttggacgctt gatacgcgtc
    73201 atatacttgg accacacgcg ttctgcttct gtcactggct cacgttcgcc acggcatact
    73261 gctacgaaga gtttctcttc ctcggtaacc ggctcgcgtt tgccaagatc caactcattg
    73321 aaggcataac catgacgctc aagcagttgt gcctctttga tggtgaagtc accatgacga
    73381 gagaatccac gtggataatg tttattgtcg aaatatcgat tagtcgtcgt aaagctttcc
    73441 gccatcctgc acgctcctaa ttctttgacc gagctagtta tggcgcggag tattagttac
    73501 gcttgacaga gtgtaaaaca aaacatttaa atcataacga caaataattt tgcggagagc
    73561 actgtggata cggaattgtt aaaaactttc ctggaagtta gccgaacgcg tcactttggt
    73621 cgagcggctg aatcgctcta tctgacccaa tcagccgtga gctttcgaat cagacaactg
    73681 gaaaatcaac tgggtgtgaa tcttttcacc cgccacagaa acaatatccg tttaaccgct
    73741 gccggtgaaa aactactgcc ttatgcagaa acgctcatga gcacctggca ggccgcccgt
    73801 agggaggtgg cgcatacctc acgacataac gagttttcta tcggtgccag cgcctcattg
    73861 tgggaatgta tgcttaatca gtggctggga cgcttgtatc aaaatcaaga tgcccataca
    73921 ggcttacagt tcgaagcgcg aattgcccaa cgacagtctc tggtaaaaca gctgcatgaa
    73981 cgccagcttg atcttcttat taccactgaa gcgcccaaaa tggacgaatt tagtagtcag
    74041 ttgctggggt atttcacttt agcgctttat accagtgccc cttctaaact aaagggagat
    74101 cttaactatc tgcgactgga gtgggggcca gattttcaac agcatgaggc aggtttgatc
    74161 ggtgctgacg aagttcccat tctgacaacc agttctgctg aactggcaca gcaacagatt
    74221 gcgatgctta atggttgcac ctggctaccc gtcagttggg cgcgtaaaaa gggcggcctg
    74281 cataccgttg tcgatagcac aacactttca cggccgcttt atgccatatg gctgcaaaat
    74341 agcgataaaa atgcgttgat tcgcgatcta ttgaaaatta acgtgctgga tgaagtgtat
    74401 taatctgaat ggctggcaag gatgctggta gaaggattta cttcggagag gttatttcag
    74461 ataaaaaaaa tccttagctt tcgctaagga tgatttctgg caggggcgga gagactcgaa
    74521 ctcccaacac ccggttttgg agaccggtgc tctaccaatt gaactacgcc cctaattagg
    74581 gtggcggaac ggacgggact cgaacccgcg accccctgcg tgacaggcag gtattctaac
    74641 cgactgaact accgctccac cgaattctt
//
LOCUS       DAMZFT010000008        73131 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.008, whole genome shotgun sequence.
ACCESSION   DAMZFT010000008 DAMZFT010000000
VERSION     DAMZFT010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 73131)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 73131)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..73131
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.008"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(<1..240)
                     /locus_tag="PN977_000631"
     CDS             complement(<1..240)
                     /locus_tag="PN977_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HDI6040698.1"
                     /translation="MVWLANPERYGQMQYRYCGKSGLRLPALSLGLWHNFGHVNALES
                     QRAILRKAFDLGITHFDLANNYGPPPGSAEENFGRL"
     gene            397..1284
                     /locus_tag="PN977_000632"
     CDS             397..1284
                     /locus_tag="PN977_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311911.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HDI6040699.1"
                     /translation="MPRLTAKDFPQELLDYYDYYAHGKISKREFLNLAAKYAVGGMTA
                     LALFDLLKPNYALATQVEFTDPEIVAEYITYPSPNGHGEVRGYLVKPAKMSGKTPAVV
                     VVHENRGLNPYIEDVARRVAKAGYIALAPDGLSSVGGYPGNDDKGRELQQQVDPTKLM
                     NDFFAAIEFMQRYPQATGKVGITGFCYGGGVSNAAAVAYPELACAVPFYGRQAPTADV
                     AKIEAPLLLHFAELDTRINEGWPAYEAALKANNKVYEAYIYPGVNHGFHNDSTPRYDK
                     SAADLAWQRTLKWFDKYLS"
     gene            1403..1690
                     /locus_tag="PN977_000633"
     CDS             1403..1690
                     /locus_tag="PN977_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2623 domain-containing protein"
                     /protein_id="HDI6040700.1"
                     /translation="MNNHFGKGLMAGLKATHADSAVNVTKYCADYKRGFVLGYSHRMY
                     EKTGDRQLSAWEAGILTRRYGLDKEMVMDFFRENNSCSTLRFFMAGYRLEN"
     gene            1879..2997
                     /gene="hybO"
                     /locus_tag="PN977_000634"
     CDS             1879..2997
                     /gene="hybO"
                     /locus_tag="PN977_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 small subunit"
                     /protein_id="HDI6040701.1"
                     /translation="MTGDNTLIHSHGINRRDFMKLCAALAATMGLSSKAAAEMAESVT
                     NPQRPPVIWIGAQECTGCTESLLRATHPTVENLVLETISLEYHEVLSAAFGHQVEENK
                     HNALEKYKGQYVLVVDGSIPLKDNGIYCMVAGEPIVDHIRKAAEGAAAIIAIGSCSAW
                     GGVAAAGVNPTGAVSLQEVLPGKTVINIPGCPPNPHNFLATVAHIITYGKPPKLDDKN
                     RPTFAYGRLIHEHCERRPHFDAGRFAKEFGDEGHREGWCLYHLGCKGPETYGNCSTLQ
                     FCDVGGVWPVAIGHPCYGCNEEGIGFHKGIHQLANVENQTPRSQKPDVNAKEGGNVSA
                     GAIGLLGGVVGLVAGVSVMAVRELGRQQKKDNADSRGE"
     gene            3000..3986
                     /gene="hybA"
                     /locus_tag="PN977_000635"
     CDS             3000..3986
                     /gene="hybA"
                     /locus_tag="PN977_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 operon protein HybA"
                     /protein_id="HDI6040702.1"
                     /translation="MNRRNFIKAASCGALLTGALPSVSHAAAENRPPIPGSLGMLYDS
                     TLCVGCQACVTKCQDINFPERNPQGEQTWSNNDKLSPYTNNIIQVWTSGTGVNKDQEE
                     NGYAYIKKQCMHCVDPNCVSVCPVSALKKDPKTGIVHYDKDVCTGCRYCMVACPYNVP
                     KYDYNNPFGALHKCELCNQKGVERLDKGGLPGCVEVCPAGAVIFGTREELMAEAKKRL
                     ALKPGSEYHYPRQTLKSGDTYLHTVPKYYPHLYGEKEGGGTQVLVLTGVPYENLDLPK
                     LDDLSTGARSENIQHTLYKGMMLPLAVLAGLTVLVRRNTKNDHHDGGDDHES"
     gene            3976..5154
                     /gene="hybB"
                     /locus_tag="PN977_000636"
     CDS             3976..5154
                     /gene="hybB"
                     /locus_tag="PN977_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase cytochrome b subunit"
                     /protein_id="HDI6040703.1"
                     /translation="MSHDPQPLGGKIISKPVMIFGPLIVICMLLIVKRLVFGLGSVSD
                     LNGGFPWGVWIAFDLLIGTGFACGGWALAWAVYVFNRGQYHPLVRPALLASLFGYSLG
                     GLSITIDVGRYWNLPYFYIPGHFNVNSVLFETAVCMTIYIGVMALEFAPALFERLGWK
                     VSLQRLNKVMFFIIALGALLPTMHQSSMGSLMISAGYKVHPLWQSYEMLPLFSLLTAF
                     IMGFSIVIFEGSLVQAGLRGNGPDEKSLFVKLTNTISVLLAIFIVLRFGELIYRDKLS
                     LAFAGDFYSVMFWIEVLLMLFPLVVLRVAKLRNDSRMLFLSALSALLGCATWRLTYSL
                     VAFNPGGGYAYFPTWEELLISIGFVAIEICAYIVLIRLLPILPPLKQNDHNRHEASKA
                     "
     gene            5151..6854
                     /gene="hybC"
                     /locus_tag="PN977_000637"
     CDS             5151..6854
                     /gene="hybC"
                     /locus_tag="PN977_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 large subunit"
                     /protein_id="HDI6040704.1"
                     /translation="MSQRITIDPVTRIEGHLRIDCEIENGVVSKAWASGTMWRGMEEI
                     VKNRDPRDAWMIVQRICGVCTTTHALSSVRAAESALNIDVPVNAQYIRNIILAAHTTH
                     DHIVHFYQLSALDWVDITSALQADPTKASEMLKGVSTWHLNSPEEFTKVQNKIKDLVA
                     SGQLGIFANGYWGHPAMKLPPEVNLIAVAHYLQALECQRDANRVVALLGGKTPHIQNL
                     AVGGVANPINLDGLGVLNLERLMYIKSFIDKLSDFVEQVYKVDTAVIAAFYPEWLTRG
                     KGAVNYLSVPEFPTDSKNGSFLFPGGYIENADLSSYRPITSHSDEYLIKGIQESAKHS
                     WYKDEAPQAPWEGTTIPAYDGWSDDGKYSWVKSPTFYGKTVEVGPLANMLVKLAAGRE
                     STQNKLNEIVAIYQKLTGNTLEVAQLHSTLGRIIGRTVHCCELQDILQNQYSALITNI
                     GKGDHTTFVKPNIPATGEFKGVGFLEAPRGMLSHWMVIKDGIISNYQAVVPSTWNSGP
                     RNFNDDVGPYEQSLVGTPVADPNKPLEVVRTIHSFDPCMACAVHVVDADGNEVVSVKV
                     L"
     gene            6854..7348
                     /locus_tag="PN977_000638"
     CDS             6854..7348
                     /locus_tag="PN977_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HyaD/HybD family hydrogenase maturation
                     endopeptidase"
                     /protein_id="HDI6040705.1"
                     /translation="MRILVLGVGNILLTDEAIGVRIVEALEQRYILPDYVEILDGGTA
                     GMELLGDMANRDHLIIADAIVSKKNAPGTMMILRDEEVPALFTNKISPHQLGLADVLS
                     ALRFTGEFPKKLTLVGVIPESLEPHIGLTPTVEAMIEPALEQVLAALRESGVEAIPRE
                     AIHD"
     gene            7341..7829
                     /gene="hybE"
                     /locus_tag="PN977_000639"
     CDS             7341..7829
                     /gene="hybE"
                     /locus_tag="PN977_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-2 assembly chaperone"
                     /protein_id="HDI6040706.1"
                     /translation="MTEEIAGFQTSPKAQVQAAFEEIARRSMHDLSFLHPSMPVYVSD
                     FTLFEGQWTGCVITPWMLSAVIFPGPDQLWPLRKVSEKIGLQLPYGTMTFTVGELDSV
                     SQYLSCSLMSPLSHSMSIEEGQRLTDDCARMILSLPVTNPDVPHAGRRALLFGRRSGE
                     NA"
     gene            7822..8163
                     /gene="hypA"
                     /locus_tag="PN977_000640"
     CDS             7822..8163
                     /gene="hypA"
                     /locus_tag="PN977_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HDI6040707.1"
                     /translation="MHELSLCQSAVEIIQRQAEQHDVKRVTAVWLEIGALSCVEESAV
                     RFSFEIVCHGTVAQGCDLHIVYKPAQAWCWDCSQVVEIHQHDAQCPICHGERLRVDTG
                     DSLIVKSIEVE"
     gene            8176..8424
                     /gene="hybG"
                     /locus_tag="PN977_000641"
     CDS             8176..8424
                     /gene="hybG"
                     /locus_tag="PN977_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation factor HybG"
                     /protein_id="HDI6040708.1"
                     /translation="MCIGVPGQVLAVGEDIHQLAQVEVCGIKRDVNIALICEGNPADL
                     LGQWVLVHVGFAMSIIDEDEAKATLDALRQMDYDITSA"
     gene            complement(8494..9360)
                     /gene="yghU"
                     /locus_tag="PN977_000642"
     CDS             complement(8494..9360)
                     /gene="yghU"
                     /locus_tag="PN977_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent disulfide-bond
                     oxidoreductase"
                     /protein_id="HDI6040709.1"
                     /translation="MTDNTYQPAKVWTWDKSAGGAFANINRPVSGPTHEKTLPVGKHP
                     LQLYSLGTPNGQKVTIMLEELLALGVTGAEYDAWLIRIGDGDQFSSGFVEVNPNSKIP
                     ALRDHTHNPPIRVFESGSILLYLAEKFGYFLPQDLAKRTETLNWLFWLQGAAPFLGGG
                     FGHFYHYAPVKIEYAINRFTMEAKRLLDVLDKQLAQHKFVAGDEYTIADMAIWPWFGN
                     VVLGGVYDAAEFLDAGSYKHVQRWAKEVGERPAVKRGRIVNRTNGPLNEQLHERHDAS
                     DFETNTEDKRQG"
     gene            9565..11424
                     /gene="gss"
                     /locus_tag="PN977_000643"
     CDS             9565..11424
                     /gene="gss"
                     /locus_tag="PN977_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutathionylspermidine
                     amidase/synthase"
                     /protein_id="HDI6040710.1"
                     /translation="MSKGTTSQDAPFGTLLGYAPGGVAIYSSDYSSLDPQEYEDDAVF
                     RSYIDDEYMGHKWQCVEFARRFLFLNYGVVFTDVGMAWEIFSLRFLREVVNDNILPLQ
                     AFPNGSPRAPVAGALLIWDKGGEFKDTGHVAIITQLHGNKVRIAEQNVIHSPLPQGQQ
                     WTRELEMVVENGCYTLKDTFDDTTILGWMIQTEDTDYSLPQPEIAGELLKISGARLEN
                     KGQFDGKWLDEKDPLQNAYVQANGQVINQDPYHYYTITESAEQELIKATNELHLMYLH
                     ATDKVLKDDNLLALFDIPKILWPRLRLSWQRRRHHMITGRMDFCMDERGLKVYEYNAD
                     SASCHTEAGLILERWAEQGYKGNGFNPAEGLINELAGAWKHSRARPFVHIMQDKDIEE
                     NYHAQFMEQALHQAGFETRILRGLDELGWDAAGQLIDGEGRLVNCVWKTWAWETAFDQ
                     IREVSDREFAAVPIRTGHPQNEVRLIDVLLRPEVLVFEPLWTVIPGNKAILPILWSLF
                     PHHRYLLDTDFTVNDELVKTGYAVKPIAGRCGSNIDLVSHHEEVLDKTSGKFAEQKNI
                     YQQLWCLPKVDGKYIQVCTFTVGGNYGGTCLRGDESLVIKKESDIEPLIVVKK"
     gene            11716..13215
                     /gene="pitB"
                     /locus_tag="PN977_000644"
     CDS             11716..13215
                     /gene="pitB"
                     /locus_tag="PN977_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitB"
                     /protein_id="HDI6040711.1"
                     /translation="MLNLFVGLDIYTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMQPQLAVVMAAFFNFFGVLLGGLSVAYAIVHMLPTDLLLNMGSTHGLAMVFSMLL
                     AAIIWNLGTWFFGLPASSSHTLIGAIIGIGLTNALLTGSSVMDALNLREVTKIFSSLI
                     VSPIVGLVIAGGLIFLLRRFWSGTKKRDRIHRIPEDRKKKKGKRKPPFWTRIALIVSA
                     AGVAFSHGANDGQKGIGLVMLVLVGIAPAGFVVNMNASGYEITRTRDAVTNFEHYLQQ
                     HPELPQKLITMEPPLPATSTDGTQVTEFHCHPANTFDAIARVKTMLPGNMESYEPLSV
                     SQRSQLRRIMLCISDTSAKLAKLPGVSKEDQNLLKKLRSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVAMTIGEKIGKRGMTYAQGMAAQMTAAVSIGLASYIGMPVSTTHVL
                     SSAVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAIFLSGGLYWIALQLI"
     gene            complement(13264..13956)
                     /locus_tag="PN977_000645"
     CDS             complement(13264..13956)
                     /locus_tag="PN977_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate kinase"
                     /protein_id="HDI6040712.1"
                     /translation="MQSITPPLIAVIGSDGSGKSTVCEHLITVVEKYGAAERVHLGKQ
                     AGNVGRAVTKLPLMGKSLHKTIERNQVKTAKKLPGPVPALVITAFVARRLLRFRHMLA
                     CRRRGLIVLTDRYPQDQIPGAYDGTVFPPNVEGGRFVSWLASQERKAFHWMASHKPDL
                     VIKLNVDLEVACARKPDHKRESLARKIAITPQLTFGGAQLVDINANQPLEQVLVDAEK
                     AIMDFMTARGYH"
     gene            14130..14843
                     /locus_tag="PN977_000646"
     CDS             14130..14843
                     /locus_tag="PN977_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate kinase"
                     /protein_id="HDI6040713.1"
                     /translation="MNQRNMSIINSTPVRVIAIVGCDGSGKSTLTASLVNELAATMPT
                     EHIYLGQSSGRIGEWISQLPVIGAPFGRYLRSKAAHVHEKPLTPPGNITALVIYLLSC
                     WRAYKFRKMLCKSQQGYLLITDRYPQVEVPGFRFDGPQLAKTTGGNGWIKMLRQRELK
                     LYQWMASYLPVLLIRLGIDEQTAFARKPDHQLAALQEKIAVTPQLTFNGAKILELDGR
                     QPADEILQASLRAIHAALS"
     gene            14875..15633
                     /locus_tag="PN977_000647"
     CDS             14875..15633
                     /locus_tag="PN977_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6040714.1"
                     /translation="MDALQTQTVNSTTAPQPNYIPGLIAVVGCDGTGKSTLTTDLVKS
                     LQQHWQTERRYLGLLSGEDGDKIKRLPLVGVWLERRLAAKSSKTQSMKTKSPALWAAV
                     IMYCFSLRRMANLRKVQRLAQSGVLVVSDRFPQAEISGFYYDGPGIGVERATGKISMF
                     LAQRERRLYQQMAQYRPELIIRLGIDIDTAISRKPDHDYAELQDKIGVMSTIGYNGTK
                     ILEIDSRAPYSEVLEQAQKAVSLVAIVSDRRSLT"
     gene            15679..17028
                     /locus_tag="PN977_000648"
     CDS             15679..17028
                     /locus_tag="PN977_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HDI6040715.1"
                     /translation="MAGFNIKHWFADGAFRTIIRNSAWLGSSNVVSALLGLLALSCAG
                     KGMTPAMFGVLVIVQSYAKSISDFIKFQTWQLVVQYGTPALTNNNPQQFRNVVSFSFS
                     LDIVSGAVAIVGGIALLPFLSHSLGLDDQSFWLAALYCTLIPSMASSTPTGILRAVDR
                     FDLIAVQQATKPFLRAAGSVVAWYFDFGFAGFVIAWYVSNLVGGTMYWWFAARELRRR
                     NIHNAFKLNLFESARHIKGAWSFVWSTNIAHSIWSARNSCSTVLVGIVLGPAAAGLFK
                     IAMTFFDAAGTPAGLLGKSFYPEVMRLDPRTTRPWLLGVKSGLLAGGIGILVALAVLI
                     VGKPLISLVFGVKYLEAYDLIQVMLGAIVISMLGFPQESLLLMAGKQRAFLVAQTIAS
                     IGYIVLLFMFCHLFGVLGAAFAYFGGQCLDVVLSLIPTLRAFFQRHSLLYNAAGEKS"
     gene            17028..17852
                     /locus_tag="PN977_000649"
     CDS             17028..17852
                     /locus_tag="PN977_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6040716.1"
                     /translation="MMRKYFPLEASERLFVAIEEDDVVDAQVSLPPTIALSCTTEIIH
                     DNYALCLQFWLNGVNRQELLRLICKQAKGDELTADERKQFKYMRARYKHLRFAQRLYL
                     KKHQAGFLFGKTTVFLGRFQDGFRNGKKNIVSYYGNLLRVYLSSPVWSLVNYSLRHSQ
                     LESVSGFIAYRQKQMHTLKEIIAKPRLTGREFHDVRKIISQQVSYYDTLRSLDPENKE
                     ALQISRFLAAINGLMGDKHDDMVADDMENRHSYDAPMALDSDIRQRLELLISRFPL"
     gene            17864..18424
                     /locus_tag="PN977_000650"
     CDS             17864..18424
                     /locus_tag="PN977_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HDI6040717.1"
                     /translation="MSSRLIIALVIMLLASGVQAHNFVTGKTVTPVYIQEGGELLLNS
                     EDEIHYQKWNSTQLAGKVRIIQYIAGRKSAKKKNSLLIKAVEAANFPQDRFQPTTIVN
                     TDDAIFGTGYFVVGKIEKNKRRYPWAQFVIDGNGLGRVAWRLPEQSSTILVLNKDGQI
                     QWAKDGSLTPEEVDHVIALAQKLINE"
     gene            18455..19525
                     /gene="lptF"
                     /locus_tag="PN977_000651"
     CDS             18455..19525
                     /gene="lptF"
                     /locus_tag="PN977_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HDI6040718.1"
                     /translation="MKLVEHYIMRGTRRLVLIIVGFLIFIFASYSAQRYLTEAANGTL
                     ALDVVLDIVFYKVLIALEMLLPVGLYVSVGVTLGQMYTDSEITAISAAGGSPGRLYKA
                     VLYLAIPLSIFVTLLSMYGRPWAYAQIYQLEQQSQSELDVRQLRAKKFNTNDNGRMIL
                     SQTVDQDNNRLTDALIYTSTANRTRIFRARSVDVVDPSPEKPTVMLHNGTAYLLDHQG
                     RDDNEQIYRNLQLHLNPLDQSPNVKRKAKSVTELARSAFPADHAELQWRQSRGLTALL
                     MALLAISLSRVKPRQGRFSTLLPLTLLFVAIFYGGDVCRTLVANGAIPLIPGLWLVPG
                     LMLMGLLMLVARDFSLLQKFSR"
     gene            19522..20601
                     /gene="lptG"
                     /locus_tag="PN977_000652"
     CDS             19522..20601
                     /gene="lptG"
                     /locus_tag="PN977_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HDI6040719.1"
                     /translation="MNVFSRYLIRHLFLGFAAAAGLLLPLFTTFNLINELDDVSPGGY
                     RWTLAVLVVLMTLPRTLVELSPFIALLGGIVGLGQLSKNSELTAIRSTGFSIFRIALV
                     ALVAGILWTVSLGAIDEWVASPLQQQALQIKSTATALGEDDDITGNMLWARRGNEFVT
                     VKSLNEQGQPVGVEIFHYRDDLSLESYIFARSASIEDDKTWILHGVNHKKWLNGKETL
                     ETSDNLAWQSAFTSMDLEELSMPGNTFSVRQLNHYIHYLQETGQPSSEYRLALWEKLG
                     QPILTLAMILLAVPFTFSAPRSPGVGSRLAVGVIVGLLTWISYQIMVNLGLLFALSAP
                     VTALGLPVAFVLVALSLVYWYDRQH"
     gene            complement(20636..21808)
                     /locus_tag="PN977_000653"
     CDS             complement(20636..21808)
                     /locus_tag="PN977_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HDI6040720.1"
                     /translation="MGLYDKYARLAGERLQFSDNGLTPFGTCIDEVYSATEGRIGNKK
                     VILAGTNNYLGLTFNHDAISEGQAALAAQGTGTTGSRMANGSYAPHLALEKEIAEFFN
                     RPTAIVFSTGYTANLGVISALADHNAVVLLDADSHASIYDACSLGGAEIIRFRHNDAK
                     DLERRMVRLGERAKEAIIIVEGIYSMLGDVAPLAEIVDIKRRLGGYLIVDEAHSFGVL
                     GATGRGLAEAVGVEDDVDIIVGTFSKSLASIGGFAVGSEAMEVLRYGSRPYIFTASPS
                     PSCIATVRSSLRTIATQPELRQKLMDNANHLYDGLQKLGYELSSHISPVVPVIIGSKE
                     EGLRIWRELISLGVYVNLILPPAAPAGITLLRCSVNAAHSREQIDAIIQAFATLKQ"
     gene            complement(21808..22056)
                     /locus_tag="PN977_000654"
     CDS             complement(21808..22056)
                     /locus_tag="PN977_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HDI6040721.1"
                     /translation="MINREIVMDYILSCLQDLVENGVEIKPDSDLVNDLGLESIKVMD
                     LLMMLEDRFDISIPINILLDVKTPAQLMETLLPWLENK"
     gene            complement(22088..23002)
                     /locus_tag="PN977_000655"
     CDS             complement(22088..23002)
                     /locus_tag="PN977_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001481799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HDI6040722.1"
                     /translation="MNQTVAVTGATGFIGKYIIDNLLACGFHVRALTRTARAHVNDNL
                     TWVRGSLEDTHSLSELVTGASAVVHCAGQVRGHKEEIFTRCNVDGSLRLMQAAKESGF
                     CQRFLFISSLAARHPELSWYANSKHVAEQQLTAMADEITLGVFRPTAVYGPGDKELKP
                     LFDWMLRGLLPRLGTPETQLSFLHVTDFAQAVGQWLSAETVQTQTYELCDGVAGGYDW
                     QRVQQLVADVRCGSVRMVGIPLPLLTCLADISTALSRLAGKEPMLTRSKIRELTHADW
                     SASNNRISEDINWFPGISLEHALRNGLF"
     gene            complement(22999..24732)
                     /locus_tag="PN977_000656"
     CDS             complement(22999..24732)
                     /locus_tag="PN977_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acyl-AMP ligase"
                     /protein_id="HDI6040723.1"
                     /translation="MVYMSNKIFTHSLPMRYADFPTLVDALDYAALSSAGMNFYDRRC
                     QLEDQLEYQTLKARAEAGAKRLLSLNLKKGDRVALIAETSSGFVEAFFACQYAGLVAV
                     PLAIPMGVGQRDSWSAKLQGLLASCQPAAIITGDEWLPLVNAATHNNNPELHVLSHAW
                     FKALPEADVALQRPVPNDIAYLQYTSGSTRFPRGVIITHREVMANLRAISHDGIKLRP
                     GDRCVSWLPFYHDMGLVGFLLTPVATQLSVDYLRTQDFAMRPLQWLKLISKNRGTVSV
                     APPFGYELCQRRVNEKDLAELDLSCWRVAGIGAEPISAEQLHQFAECFRQVNFDDKTF
                     MPCYGLAENALAVSFSDEASGVVVNEVDRDILEYQGKAVAPDAETRAVSTFVNCGKAL
                     PEHGIEIRNEAGIPVAERVVGHICISGPSLMSGYFGDQISQDEIAATGWLDTGDLGYL
                     LDGYLYVTGRIKDLIIIRGRNIWPQDIEYIAEQEPEIHSGDAIAFVTAQEKIILQIQC
                     RISDEERRGQLIHALAARIQSEFGVTADIDLLPPHSIPRTSSGKPARAEAKKRYQKAY
                     AASLHVQESLA"
     gene            25094..26236
                     /locus_tag="PN977_000657"
     CDS             25094..26236
                     /locus_tag="PN977_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001327865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HDI6040724.1"
                     /translation="MECDLLMIKIEKVINKNDLKAFIAFPSSLYPDDPNWIPPLFIER
                     NEHLSAKNPGTDHIIWQAWVAKKAGQIVGRITAQIDTLHRERYGKDTGHFGMIDAIDD
                     PQVFAALFGAAEAWLKSQGASKISGPFSLNINQESGLLIEGFDTPPCAMMPHGKPWYA
                     AHIEQLGNHKGIDLLAWWMQRTDLTFSPALKKLMDQVRKKVTIRCINRQRFAEEMQIL
                     REIFNSGWQHNWGFVPFTEHEFATMGDQLKYLVPDDMIYIAEIDSAPCAFIVGLPNIN
                     EAIADLNGSLFPFGWAKLLWRLKVSGVRTARVPLMGVRDEYQFSRIGPVIALLLIEAL
                     RDPFARRKIDALEMSWILETNTGMNNMLERIGAEPYKRYRLYEKQI"
     gene            complement(26312..27082)
                     /gene="glcC"
                     /locus_tag="PN977_000658"
     CDS             complement(26312..27082)
                     /gene="glcC"
                     /locus_tag="PN977_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GlcC"
                     /protein_id="HDI6040725.1"
                     /translation="MKDERRPICEVVAESIERLIIDGVLKVGQPLPSERRLCEKLGFS
                     RSALREGLTVLRGRGIIETAQGRDSRVARLNRVQDTSPLIHLFSTQPRTLYDLLDVRA
                     LLEGESARLAATLGTQADFVVITRCYEKMLAASENNKEISLIEHAQLDHAFHLAICQA
                     SHNQVLVFTLQSLTDLMFNSVFASVNNLYHRPQQKKQIDRQHARIYNAVLQRLPHVAQ
                     RAARDHVRTVKKNLHDIELEGHHLIRSAVPLEMNKVGM"
     gene            27333..28832
                     /gene="glcD"
                     /locus_tag="PN977_000659"
     CDS             27333..28832
                     /gene="glcD"
                     /locus_tag="PN977_000659"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcD"
                     /protein_id="HDI6040726.1"
                     /translation="MSILYEERLDGALPDVDRTSVLMALREHVPGLEILHTDEEIIPY
                     ECDGLSAYRTRPLLVVLPKQMEQVTAILAVCHRLRVPVVTRGAGTGLSGGALPLEKGV
                     LLVMARFKEILDINPVGRRARVQPGVRNLAISQAVAPHNLYYAPDPSSQIACSIGGNV
                     AENAGGVHCLKYGLTVHNLLKIEVQTLDGEALTLGSDALDSPGFDLLALFTGSEGMLG
                     VTTEVTVKLLPKPPVARVLLASFDSVEKAGLAVGDIIANGIIPGGLEMMDNLSIRAAE
                     DFIHAGYPVDAEAILLCELDGVESDVQEDCEQVNDILLNAGATDVRLAQDEAERVRFW
                     AGRKNAFPAVGRISPDYYCMDGTIPRRALPGVLEGIARLSQQYDLRVANVFHAGDGNM
                     HPLILFDANEPGEFARAEELGGKILELCVEVGGSISGEHGIGREKINQMCAQFNSDEI
                     TTFHAVKAAFDPDGLLNPGKNIPTLHRCAEFGAMHVHHGHLPFPELERF"
     gene            28832..29884
                     /gene="glcE"
                     /locus_tag="PN977_000660"
     CDS             28832..29884
                     /gene="glcE"
                     /locus_tag="PN977_000660"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcE"
                     /protein_id="HDI6040727.1"
                     /translation="MLRECDYSQALLEQVNQAISDKTPLVIQGSNSKAFLGRPVTGQT
                     LDVRCHRGIVNYDPTELVITARAGTPLVAIEAALESAGQMLPCEPPHYGEEATWGGMI
                     ACGLAGPRRPWSGSVRDFVLGTRIITGTGKHLRFGGEVMKNVAGYDLSRLMAGSYGCL
                     GVLTEISMKVLPRPRATLSLRREISLQEAMNEIAQWQLQPLPISGLCYFDNALWIRLE
                     GGEGSVKAARELLGGEEVADQFWQQLREQQLPFFSLPGTLWRISLPSDAPMMDLPGEQ
                     LIDWGGALRWLKSTADDNQIHRIARNAGGHATRFSAGDGGFAPLPAPLFRYHQQLKQQ
                     LDPCGVFNPGRMYAEL"
     gene            29895..31118
                     /gene="glcF"
                     /locus_tag="PN977_000661"
     CDS             29895..31118
                     /gene="glcF"
                     /locus_tag="PN977_000661"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcF"
                     /protein_id="HDI6040728.1"
                     /translation="MQTQLTEEMRQNARALEADSILRACVHCGFCTATCPTYQLLGDE
                     LDGPRGRIYLIKQVLEGNEVTLKTQEHLDRCLTCRNCETTCPSGVRYHNLLDIGRDIV
                     EQKVKRPLPERMLREGLREVVPRPAVFRALTQVGLVLRPFLPEQVRAKLPAETVKAKP
                     RPPLRHKRRVLMLEGCAQPTLSPNTNAATARVLDRLGISVMPANEAGCCGAVDYHLNA
                     QEKGLARARNNIDAWWPAIEAGAEAILQTASGCGAFVKEYGQMLKNDALYADKARQVS
                     ELAVDLVELLREEPLEKLAIRGDKKLAFHCPCTLQHAQKLNGEVEKVLLRLGFTLTDV
                     PDSHLCCGSAGTYALTHPDLARQLRDNKMNALESGKPEMIVTANIGCQTHLASAGRTS
                     VRHWIEIVEQALEKE"
     gene            31123..31527
                     /locus_tag="PN977_000662"
     CDS             31123..31527
                     /locus_tag="PN977_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein GlcG"
                     /protein_id="HDI6040729.1"
                     /translation="MKTKVILSQQMASAIIAAGQEEAQKNNWSVSIAVADDGGHLLAL
                     SRMDDCAPIAAYISQEKARTAALGRRETKGYEEMVNNGRTAFVTAPLLTSLEGGVPVV
                     VDGQIIGAVGVSGLTGAQDAQVAKAAAAVLAK"
     gene            31549..33720
                     /gene="glcB"
                     /locus_tag="PN977_000663"
     CDS             31549..33720
                     /gene="glcB"
                     /locus_tag="PN977_000663"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase G"
                     /protein_id="HDI6040730.1"
                     /translation="MSQTITQGRLRINANFKRFVDEEVLPGTGLDTAAFWRNFDEIVH
                     DLAPENRQLLAERDRIQAALDEWHRSNPGPVKDKEAYKSFLRELGYLVPQPERVTVET
                     TGIDSEITSQAGPQLVVPAMNARYALNAANARWGSLYDALYGSDIIPQEGAMVSGYDP
                     QRGEQVIAWVRRFLDESLPLENGSYQDVLAFKVVDKQLRIQLKNGKETTLRTPAQFVG
                     YRGDAAAPTCILLKNNGLHIELQIDANGRIGKDDPAHINDVIVEAAISTILDCEDSVA
                     AVDAEDKILLYRNLLGLMQGTLQEKMEKNGRQIVRKLNDDRHYTAADGSEISLHGRSL
                     LFIRNVGHLMTIPVIWDSEGNEIPEGILDGVMTGAIALYDLKVQKNSRTGSVYIVKPK
                     MHGPQEVAFANKLFTRIETMLGMAPNTLKMGIMDEERRTSLNLRSCIAQARNRVAFIN
                     TGFLDRTGDEMHSVMEAGPMLRKNQMKSTPWIKAYERNNVLSGLFCGLRGKAQIGKGM
                     WAMPDLMADMYSQKGDQLRAGANTAWVPSPTAATLHALHYHQTNVQSVQANIAQSEFN
                     AEFEPLLDDLLTIPVAENANWSVEEIQQELDNNVQGILGYVVRWVEQGIGCSKVPDIH
                     NVALMEDRATLRISSQHIANWLRHGILTKEQVQASLENMAKVVDQQNAGDPAYRPMAG
                     NFANSCAFKAASDLIFLGVKQPNGYTEPLLHAWRLREKESH"
     gene            34075..35757
                     /gene="glcA"
                     /locus_tag="PN977_000664"
     CDS             34075..35757
                     /gene="glcA"
                     /locus_tag="PN977_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate permease GlcA"
                     /protein_id="HDI6040731.1"
                     /translation="MVTWTQMYMPMGGLGLSALVALIPIIFFFVALAVLRLKGHVAGA
                     ITLILSILIAIFAFKMPIDMAFAAAGYGFIYGLWPIAWIIVAAVFLYKLTVASGQFDI
                     IRSSVISITDDQRLQVLLIGFSFGALLEGAAGFGAPVAITGALLVGLGFKPLYAAGLC
                     LIANTAPVAFGALGVPILVAGQVTGIDPFHIGAMAGRQLPFLSVLVPFWLVAMMDGWK
                     GVKETWPAALVAGGSFAVTQFFTSNYIGPELPDITSALVSIVSLALFLKVWRPKNTET
                     AISMGQSAGAMVVNKPSSGGPVPSEYSLGQIIRAWSPFLILTVLVTIWTMKPFKALFA
                     PGGAFYSLVINFQIPHLHQQVLKAAPIVAQPTPMDAVFKFDPLSAGGTAIFIAAIISI
                     FILGVGIKKGIGVFAETLISLKWPILSIGMVLAFAFVTNYSGMSTTLALVLAGTGVMF
                     PFFSPFLGWLGVFLTGSDTSSNALFGSLQSTTAQQINVSDTLLVAANTSGGVTGKMIS
                     PQSIAVACAATGMVGRESELFRYTVKHSLIFASVIGIITLLQAYVFTGMLVS"
     gene            36242..40798
                     /gene="sslE"
                     /locus_tag="PN977_000665"
     CDS             36242..40798
                     /gene="sslE"
                     /locus_tag="PN977_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein metalloprotease SslE"
                     /protein_id="HDI6040732.1"
                     /translation="MNKKFKYKKSLLAAILSATLLAGCDGSGSGSSSDTPPVDSGTGS
                     LPEVKPDPTPNPEPTPEPTPDPEPTPEPTPDPEPEPEPEPVPTKTGYLTLGGSQRITG
                     ATCNGESSDGFTFTPGEDVTCVAGNTTIATFDTQSEAARSLRAVEKVSFSLEDAQELA
                     ASDDKKSNAVSLVTSSNSCPANTEQVCLTFSSVIESKRFDSLYKQIDLAPEEFKKLVN
                     EEVENNAATDKAPSTHTSPVVPVTTPGTKPDLNASFVSANAEQFYQYQPTEIILSEGR
                     LVDSMGNGVVGVNYYTSSGRGVTGENGKFNFSWGETISFGIDTFELGSVRGNKSTIAL
                     TELGDEVRGANIDQLIHRYSQAGKNDEREVPDVVRKVFAEYPNVINEIINLSLSNGEA
                     LSEGDQTFERTNEFLEQFESGQAKEIDTAICDSLGGCNSQRWFSLTARNVNDGQIQGV
                     INKLWGVDTNYKSVSKFHVFHDSTNFYGSTGNARGQAVVNISNAAFPILMARNDKNYW
                     LAFGEKRAWDKNELAYITEAPSLVEPENVTRDTATFNLPFISLGQVGEGKLMVIGNPH
                     YNSILRCPNGYSWEGGVDKNGQCTRNSDSNDMKHFMQNVLRYLSDDKWTPDAKASMTV
                     GTNLDTVYFKRHGQVTGNSAEFGFHPDFAGISVEHLSSYGDLDPQEMPLLILNGFEYV
                     TQVGNDPYAIPLRADTSKPKLTQQDVTDLIAYLNKGGSVLIMENVMSNLKEESASGFV
                     RLLDAAGLSMALNKSVVNNDPQGYPNRVRQQRATGIWVYERYPAVDGALPYTIDSKTG
                     EVKWKYQVENKPDDKPKLEVASWLEDVDGKQETRYAFIDEADHKTEDSLKAAKAKIFE
                     KFPGLKECKDPTYHYEVNCLEYRPGTGVPVTGGMYVPQYTQLSLNADTAKAMVQAADL
                     GTNIQRLYQHELYFRTNGRKGERLSSVELERLYQNMSVWLWNKIEYRYENDKDDELGF
                     KTFTEFLNCYANDAYTGGTQCSDELKKSLVDNNMIYGEKSVNKAGMMNPSYPLNYMEK
                     PLTRLMLGRSWWDLNIKVDVEKYPGAVSAEGEKVTETISLYSNPTKWFAGNMQSTGLW
                     APAQKEVTIESKANVPVTVTVALADDLTGREKHEVALNRPPKVTKTYELKANGEVKFT
                     VPYGGLIYIKGNSPKNESAEFTFTGVVKAPFYKDGAWKNALNSPAPLGELESDAFVYT
                     TPKKNLEASNYKGGQEQFAEELDTFASSMNDFYGRDGENGKHRMFTYKNLTGHKHRFT
                     NDVQISIGDAHSGYPVMNSSFSTNSTTLPTTPLNDWLIWHEVGHNAAETPLTVPGATE
                     VANNVLALYMQDRYLGKMNRVADDITVAPEYLEESNGQAWARGGAGDRLLMYAQLKEW
                     AEKNFDIKTWYPDGNLPAFYSEREGMKGWNLFQLMHRKARGDEVGKTKFGERNYCAES
                     NGNAADKLMLCASWVAQTDLSEFFKKWNPGANAYQLPGASEMNFEGGVSQSAYETLAA
                     LNLPKPQQGPETINQVTEHKMSAE"
     gene            40928..41737
                     /gene="pppA"
                     /locus_tag="PN977_000666"
     CDS             40928..41737
                     /gene="pppA"
                     /locus_tag="PN977_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417446.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase PppA"
                     /protein_id="HDI6040733.1"
                     /translation="MLFDVFQQYPAGMPILATVGGLIIGSFLNVVIWRYPIMLRQQMA
                     EFHGEMPSTQSKINLALPRSHCPHCQQTIRIRDNIPLLSWLMLKGRCRDCQAKISKRY
                     PLVELLTALAFLLASLVWPESGWGLAVMILSAWLIAASVIDLDNQWLPDVFTQGVLWT
                     GLIAAWAQQSPLTLQDAVTGVLVGFITFYSLRWIAGIVLRKEALGMGDVLLFAALGGW
                     VGPLSLPNVALIASCCGLIYAVITKRGSTTLPFGPCLSLGGIATLYLQALF"
     gene            41803..42213
                     /gene="gspS2"
                     /locus_tag="PN977_000667"
     CDS             41803..42213
                     /gene="gspS2"
                     /locus_tag="PN977_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system pilot lipoprotein
                     GspS-beta"
                     /protein_id="HDI6040734.1"
                     /translation="MSIKQMPGRVLISLLLSVTGLLSGCAGHNENASLLAKKQAQNIS
                     QNLPIKSAGYTLVLAQSSGTTVKMTIISEAGTQTTQTPDAFLTSYQRQMCADPTVKLM
                     ITEGINYSITINDTRTGNQYQRKLDRTTCGIVKA"
     gene            42231..43190
                     /gene="gspC"
                     /locus_tag="PN977_000668"
     CDS             42231..43190
                     /gene="gspC"
                     /locus_tag="PN977_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspC"
                     /protein_id="HDI6040735.1"
                     /translation="MAQVVFRDARIYLIQWLTKIRHTLNQRQSLNTDKEHLRKIARGM
                     FWLMLLIISAKVAHSLWRYFSFSAEYTAVSPSANKPPRADAKTFDKNDVQLISQQNWF
                     GKYQPVAAPVKQPEPVPVAETRLNVVLRGIAFGARPGAVIEEGGKQQVYLQGERLGSH
                     NAVIEEINRDHVMLRYQGKMERLSLAEEKRPTIAVTSKKAVSDEAKQAVAEPAASAPV
                     EIPAAVRQALAKDPQKIFNYIQLTPVRKEGIVGYAVKPGADRSLFDASGFKEGDIAIA
                     LNQQDFTDPRAMIALMRQLPSMDSIQLTVLRKGARYDISIALR"
     gene            43220..45280
                     /gene="gspD"
                     /locus_tag="PN977_000669"
     CDS             43220..45280
                     /gene="gspD"
                     /locus_tag="PN977_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020234996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system secretin GspD"
                     /protein_id="HDI6040736.1"
                     /translation="MFWRDMTLSVWRKKTTGLKTKKRLLPLVLAAALCSSPVWAEEAT
                     FTANFKDTDLKSFIETVGANLNKTIIMGPGVQGKVSIRTMTPLNERQYYQLFLNLLEA
                     QGYAVVPMENDVLKVVKSSAAKVEPLPLVGEGSDNYAGDEMVTKVVPVRNVSVRELAP
                     ILRQMIDSAGSGNVVNYDPSNVIMLTGRASVVERLTEVIQRVDHAGNRTEEVIPLDNA
                     SASEIARVLESLTKNSGENQPATLKSQIVADERTNSVIVSGDPATRDKMRRLIRRLDS
                     EMERSGNSQVFYLKYSKAEDLVDVLKQVSGTLTAAKEEAEGTVGSGREVVSIAASKHS
                     NALIVTAPQDIMQSLQSVIEQLDIRRAQVHVEALIVEVAEGSNINFGVQWASKDAGLM
                     QFANGTQIPIGTLGAAISQAKPQKGSTVISENGATTINPDTNGDLSTLAQLLSGFSGT
                     AVGVVKGDWMALVQAVKNDSSSNVLSTPSITTLDNQEAFFMVGQDVPVLTGSTVGSNN
                     SNPFNTVERKKVGIMLKVTPQINEGNAVQMVIEQEVSKVEGQTSLDVVFGERKLKTTV
                     LANDGELIVLGGLMDDQAGESVAKVPLLGDIPLIGNLFKSTADKKEKRNLMVFIRPTI
                     LRDGMAADGVSQRKYNYMRAEQIYRDEQGLSLMPHTAQPILPAQNQALPPEVRAFLNA
                     GRTR"
     gene            45280..46773
                     /gene="gspE"
                     /locus_tag="PN977_000670"
     CDS             45280..46773
                     /gene="gspE"
                     /locus_tag="PN977_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001525635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system ATPase GspE"
                     /protein_id="HDI6040737.1"
                     /translation="MVPVAQETTANTVRLPYSFSRRFSLVAWCEASLEILHVHPLSLS
                     VLQELQRGLNAPFTLRQIDGAEFEQRLNAVWQRDSSEARQLMEDLGSAEDFFTLAEEL
                     PETEDLLESDDDAPIIKLINAMLAEAIKEGASDIHIETFEKSLVIRFRVDGTLHEMLR
                     PGRKLASLLVSRIKVMARLDIAEKRVPQDGRIALLLGGRAIDVRVSTMPSAWGERVVL
                     RLLDKNQARLTLERLGLSHELTAQLRQLLHKPHGIFLVTGPTGSGKSTTLYAGLQELN
                     NHSRNILTVEDPIEYMIEGIGQTQVNTRVGMTFARGLRAILRQDPDVVMVGEIRDTET
                     AEIAVQASLTGHLVLSTLHTNTAVGAITRLQDMGVEPFLLSSSLTGVMAQRLVRTLCP
                     DCRQALPATDEEKRLLGITDARTVTLYHPQGCPACNHKGFRGRTAIHELIVVDATLRD
                     LIHRQAGELELERYVRQHSAGIRSNGIEKVLAGETSLDEVLRVTMEA"
     gene            46773..47996
                     /gene="gspF"
                     /locus_tag="PN977_000671"
     CDS             46773..47996
                     /gene="gspF"
                     /locus_tag="PN977_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001173399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system inner membrane protein
                     GspF"
                     /protein_id="HDI6040738.1"
                     /translation="MALFYYQALERNGRKTKGMIEADSARHARQLLRGKELIPVHIEA
                     RMNTSSGGMLQRRRHAHRRVAAADLALFTRQLATLVQAAMPLETCLQAVSEQSEKLHV
                     KSLGMALRSRIQEGYTLSDSLREHPRVFDSLFCSMVAAGEKSGHLDVVLNRLADYTEQ
                     RQRLKSRLLQAMLYPLVLLVVATGVVTILLTAVVPKIIEQFDHLGHALPASTRMLIAM
                     SDALQASGVYWLAGLLGLLVLGQRLLKNPAMRLRWDKTLLRLPVTGRVARGLNTARFS
                     RTLSILTASSVPLLEGIQTAAAVSANRYVEQQLLLAADRVREGSSLRAALAELRLFPP
                     MMLYMIASGEQSGELETMLEQAAVNQEREFDTQVGLALGLFEPALVVMMAGVVLFIVI
                     AILEPMLQLNNMVGM"
     gene            48013..48468
                     /gene="gspG"
                     /locus_tag="PN977_000672"
     CDS             48013..48468
                     /gene="gspG"
                     /locus_tag="PN977_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003912123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system major pseudopilin GspG"
                     /protein_id="HDI6040739.1"
                     /translation="MNSLSRTQKPRAGFTLLEVMVVIVILGVLASLVVPNLLGNKEKA
                     DRQKAISDIVALENALDMYRLDNGRYPTTEQGLEALIQQPANMADSRNYRTGGYIKRL
                     PKDPWGNDYQYLSPGEKGLFDVYTLGADGQENGEGAGADIGNWNLQEFQ"
     gene            48472..49035
                     /gene="gspH"
                     /locus_tag="PN977_000673"
     CDS             48472..49035
                     /gene="gspH"
                     /locus_tag="PN977_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001394829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspH"
                     /protein_id="HDI6040740.1"
                     /translation="MPERGFTLLEIMLVIFLIGLASAGVVQTFATDSESPAKKAAQDF
                     LTRFAQFKDRAVIEGQTLGVLIDPPGYQFMQRRYGQWLPVSSTRLSAQVTVPKQVQML
                     LQPGSDIWQKEYALELQRRRLTLHDIELELQKEAKKKTPQIRFSPFEPATPFTLRFYS
                     AAQNACWAVKLAHNGVLSLNQCDERMP"
     gene            49032..49403
                     /gene="gspI"
                     /locus_tag="PN977_000674"
     CDS             49032..49403
                     /gene="gspI"
                     /locus_tag="PN977_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001709020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspI"
                     /protein_id="HDI6040741.1"
                     /translation="MKRGFTLLEVMLALAIFALAATAVLQIASGALSNQQILEEKTVA
                     GWVAENQTALLYLMTREQRAVRHQGESDMAGSRWYWRTTPLNTGNVLLQAVDIEVSLH
                     DDFSPVIQSRRAWFSAVGGQQ"
     gene            49400..50005
                     /gene="gspJ"
                     /locus_tag="PN977_000675"
     CDS             49400..50005
                     /gene="gspJ"
                     /locus_tag="PN977_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001255030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspJ"
                     /protein_id="HDI6040742.1"
                     /translation="MRRARAGFTLLEMLVAIAIFASLALMAQQVTNGVTRVNSAVAGH
                     DQKLNLMQQTMSFLNHDLTQMMPRPVRGDQGQREPALLAGAGVLASESEGMRFVRGGV
                     VNPLMRLPRSNLLTVGYRIHGGYLERLAWPLTDAAGSVKPTTQKLIPADSLRLQFHDG
                     TRWQESWSSVQAIPVAVRITLHSPQWGEIERIWLLRGPQLS"
     gene            50002..50979
                     /gene="gspK"
                     /locus_tag="PN977_000676"
     CDS             50002..50979
                     /gene="gspK"
                     /locus_tag="PN977_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001554494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspK"
                     /protein_id="HDI6040743.1"
                     /translation="MITLPPKRGMALVVVLVLLAVMMLVTITLSGRMQQQLGRTRSQQ
                     EYQQALWYSASAESLALSALSLSLKNEKRVHLAQPWASGPRFFPLPQGQIAVTLRDAQ
                     ACFNLNALAQPTTASRPLAVQQLIALISRLDVPAYRAELIVESLWEFIDEDRSVQTRL
                     GREDSEYLARSVPFYAANQPLTDISEIRVVQGMDAGLYQKLKPLVCALPMARQQININ
                     TLDVTQSVILEALFDPWLSPVQARALLQQRPAKGWEDVDQFLAQPLLADVNERTKKQL
                     KTVLSVDSNYFWLRSDITVNEIELTMNSLIVRMGPQHFSVLWHQTGESE"
     gene            50976..52154
                     /gene="gspL"
                     /locus_tag="PN977_000677"
     CDS             50976..52154
                     /gene="gspL"
                     /locus_tag="PN977_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000097227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspL"
                     /protein_id="HDI6040744.1"
                     /translation="MSSMLEIYFPLCAADPIRWQRRTPDVEHGIWPDVADEYLQQWLQ
                     TDTIRLYIPGEWISVWQVELPDVPRKQIPTILPALLEEELNQDIDELHFAPLKIDQQL
                     ATVAVIHQQHMRNIAQWLQANGITRATVAPDWMSIPCGFMAGDAQRVICRIDECRGWS
                     AGRALAPVMFRAQLNEQNLPISLTVVGIAPEELSAWAGADAERLTVTALPAITTYGES
                     EGNLLTGPWQPRVSYRKQWARWRVMILPILLILVALAVERGVTLWSVSEQVAQSRTQA
                     EKQFLTLFPEQKRIVNLRSQVTMALKKYRPQADDTRLLAELSAIASTLKSASLTDIEM
                     RGFTFDQKRQTLHLQLRAANFASFDKLRSALAADYVVQQDALQKEGDAVSGGVTLRRK
                     "
     gene            52156..52692
                     /gene="yghD"
                     /locus_tag="PN977_000678"
     CDS             52156..52692
                     /gene="yghD"
                     /locus_tag="PN977_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GspM family type II secretion system protein
                     YghD"
                     /protein_id="HDI6040745.1"
                     /translation="MLRDKFILYFQQWRERQLSRGEHWLTQHLAGRSPREKGMLLAAV
                     VFLFSAGYYVLIWQPLSERIEQQETMLQQLVAMNARLKSAAPDIIAARKSGTTTPAQV
                     SRVISDSASAHSVVIKRIAERGENIQVWIDPVVFNDLLNWLKALDEKYALRVIQIDVS
                     AGEKPGMVNVQRLEFGRG"
     gene            53743..54519
                     /locus_tag="PN977_000679"
     CDS             53743..54519
                     /locus_tag="PN977_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001544930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDI6040746.1"
                     /translation="MARSGFEVQKVTVEALFLREIRTRFGKFRLGYLWAILEPSAHLL
                     ILLGIFGYIMHRTMPDISFPVFLLNGLIPFFIFSSISNRSVGAIEANQGLFNYRPVKP
                     IDTIIARALLETLIYVAVYILLMLIVWMAGEYFEITNFLQLVLTWSLLIILSCGVGLI
                     FMVVGKTFPEMQKVLPILLKPLYFISCIMFPLHSIPKQYWSYLLWNPLVHVVELSREA
                     VMPGYISEGVSLNYLAMFTLVTLFIGLALYRTREEAMLTS"
     gene            54516..55187
                     /locus_tag="PN977_000680"
     CDS             54516..55187
                     /locus_tag="PN977_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000590255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDI6040747.1"
                     /translation="MIKIENLTKSYRTPTGRHYVFKDLNIEIPSGKSVAFIGRNGAGK
                     STLLRMIGGIDRPDSGKIITNKTISWPVGLAGGFQGSLTGRENVKFVARLYAKQEELK
                     EKIEFVEEFAELGKYFDMPIKTYSSGMRSRLGFGLSMAFKFDYYIVDEVTAVGDARFK
                     EKCAQLFKERHKESSFLMVSHSLNSLKEFCDVALFISRDHQTCLYQNINEAIKLYQND
                     ENLNI"
     gene            55199..55594
                     /locus_tag="PN977_000681"
     CDS             55199..55594
                     /locus_tag="PN977_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001233350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HDI6040748.1"
                     /translation="MRKVITFGTFDVLHIGHINILKRAKKMGDYLIVGVSSDYLNFSK
                     KQRYPVYPETERLEIIRSLKFVDEVFIEESLELKGEYIKKFKADILVMGDDWLGRFDE
                     YKELCEVSYLPRTVGISTTELISQIKRYG"
     gene            55610..56845
                     /locus_tag="PN977_000682"
     CDS             55610..56845
                     /locus_tag="PN977_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000668514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6040749.1"
                     /translation="MKFHSDIKSAIKNAKKFKGVLFVNIISYLLLTIAIRKGHSKEIL
                     KFKWLILLLGGSVAKYFVARASFLNGDYICAKKLYQEIIKVNPIHTDSYYGLADVYRL
                     IGNKLEAKGILIQVFNFSDRLKTWLLLANLVDTKEDYDELLNHWERECSYRNILKYHY
                     DVNDYLIIAALRAKLYEEALNRVKVLLGSINSEAFILPKTSNKNFSVNAAKIALLDLK
                     KILDTNKIQFFLVSGTLLGCIRDKKLLKHDKDIDIGVWSETNLSALSTKIACSGLFDI
                     APMRSPYTLRIKHVNGVAIDIFFHYRDHDSYWHAGSKLRWNNTPFNLISYGFLGNVFL
                     IPENYDLYLTENYGNWMQEKMKFDSAFDTPNHEIINMYELKIHAYKKLIINLKIGSIE
                     GVRYYEDKLNELGCIISKP"
     gene            56898..60698
                     /locus_tag="PN977_000683"
     CDS             56898..60698
                     /locus_tag="PN977_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000927649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-glycerol glycerophosphotransferase family
                     protein"
                     /protein_id="HDI6040750.1"
                     /translation="MLLTKLAKKICQSKEYINGISLYKKKEWERALLFFEKSIIKRTK
                     HAESYFKAGICNLKLHRYEAAFKYISKALELEPSHIQWKEQLEQCARHLDKLNNHMVS
                     KSSTEEDLLREKLNTDFNNPKLHDRLAEILHKKGRWWQEVDTLKNAIKLDPSIPERYF
                     KLGTALEKMGRFNEASIYYKKGLEFNKKHDAIWYYALGYSLESDETNNAENVRSSEIA
                     YATAISLDKKLNSNKFGIGAFHQYKGRWAKAIDAYEKHTSINPLCAELYYRLGLSYDR
                     CYQWDKAAENYRKALSLDENHPHWHYRLGFVLERSQKYLDAAVAYQFAAQSNTKHISI
                     WYYRSGYAYAKANKYRQSCEMYIKAYDSSQEIYASQTKAIDNSDTHLNSYRKKLKYES
                     RINTLESAISNSCINYGLWYELGKTYESSCNWNDAVIAYHEGIMRNNIYTKDYYYRIG
                     YSLFRDGNYQQACNFFRKTEIFGTPYGAPDDILTKNLTFRRNAIYTEFYETLSIEKNT
                     ILYESFHGASISCNPYALFLDIIDDQRFDNFRHIWVINNEKKIPEHLKNKKNVYFVSR
                     QSDLYMQCLASCEFLINNVSFPEYFIRKKGQRYLNTWHGTPIKFLGKDIKDEFLAHKN
                     VARNFLHTTHLLSPNTHTTNILLDRYDISNIFSGEIKELGYPRIDRTINLSSERKEYI
                     RRKINANVYDKVVLYAPTWRGIHGKATLDIEKLKNDLEKLADQDCHIVFRGHHMIEKL
                     VSEQNISGITIVPSEVDTNELLGAIDILITDYSSIAFDFFVMNRPVIYYAYDIEQYNN
                     ERGLYFPLNELPGTVCFNNVELLNALSGYLRNEIYFDASKGVDKFCKNDDGSVCGKVI
                     EWFFFEEKSILLNKNKKENILFYIGPFIPNGILSSWLNLTSVIDRDKYNISLAVDPKS
                     IHSFQERFEQFKRVSPDIQVIGTCGNMLYNIEEKWLNDKLNNQFTLASKEMYDIIDHA
                     YQREFLRLFGYSHIDHLIHFEGYNQSWVIRFANAPKDTVRNKIIFQHNDKLSEWREKF
                     PYLRVVFDFYKSYNKIVSVSEKTMELNRDNLSEFFNIEHDKFIYCDNVQNPDEVIKKS
                     DDIDTSGFNFENDKIYFITLGRLSVEKDQQKLINAFCRLQKLYPNIELLILGDGPLKI
                     DLQRQIKTLGLEKSVHLLGRISNPFPLLKRADCFVLSSNHEGQPMVLFEAMILDKPII
                     STDITGSRSALEGRSGVLVENSVDGLFNGMRDFILGRLEFKHFDIESYQKNALSMFYE
                     KCLH"
     gene            60801..61829
                     /locus_tag="PN977_000684"
     CDS             60801..61829
                     /locus_tag="PN977_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001044674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XcbB/CpsF family capsular polysaccharide
                     biosynthesis protein"
                     /protein_id="HDI6040751.1"
                     /translation="MNKTVLHINSNITTDEISLLLQASKYKYIHFEVIGKGDSCKNLI
                     EFSYHDYNLKKQLIALTNAGFMSYVHRGNVTSLVHFDEIKNLWIPVKEKKFSINSDGI
                     VYTLQRAACKINEKLLVVFSQMPIEPYSASLYRYFAKNFSTIDKYVGKNVSILRVADI
                     GGITGSFYLNTNALPTNADKIKSLILEVIEQCQIKSDDVVLYGCSKGGTAAVYHGLTN
                     NYKIVVVDPILNDEHYVNNKNDLHLIEGVFPQPKEELFKKVIDDYLINYKGNMSYFIV
                     SQNSEQYKYIMDIINPIIPYSTVANSINDKIKSHPDVGPQSIHMIVAMINMALSGIKL
                     SNGICDFV"
     gene            61835..62830
                     /locus_tag="PN977_000685"
     CDS             61835..62830
                     /locus_tag="PN977_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001208050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6040752.1"
                     /translation="MALRLNFVFILAVICSIQICSAAILSKSYSHNDIHITLPLLFNE
                     YDDVLSQYDGAKYLYPSSFALDDDNNIYIQYIFEPKQKNNILVCYNKKGNYLGYYKIP
                     SGGRGGAGEGLAIIQDYNGFNVVYVVSNTGTLQRYNMVSSLYGDSLFKERDFSINVFN
                     QFSMSQDYWLIEGYESERSIKYSRNRLELYSVSGDLLRVIYLQNNYSGYIVSDASPDA
                     KNYNKRQGIALGDGFFVASYGAFYYSKMESTYNTYQGIRVFDFSGKLLSEKIFPPDEM
                     MNVLKKMGLTVSRIENEGVIVKNNTIYSMYIYSDRYHHKTSRSEGIIILLDKFTQ"
     gene            complement(62992..64230)
                     /locus_tag="PN977_000686"
     CDS             complement(62992..64230)
                     /locus_tag="PN977_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001161825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular biosynthesis protein"
                     /protein_id="HDI6040753.1"
                     /translation="MQGNALTILLSGKKYLLLQGPMGPFFNDVAEWLESLGRNAVNVV
                     FNGGDRFYCRHRQYLAYYQTPKEFPGWLRDLHRQYDFDTILCFGDCRPLHKEAKRWAK
                     SKGLRFLAFEEGYLRPQFITVEEGGVNAYSSLPRDPDFYRKLPDMPTPHVENLKPSTM
                     KRIGHAMWYYLMGWHYRHEFPRYRHHKSFSPWYEARCWVRAYWRKQLYKVTQRKVLPR
                     LMNELDQRYYLAVLQVYNDSQIRNHSNYNDVRDYINEVMYSFSRKAPKESYLVIKHHP
                     MDRGHRLYRPLIKRLSKEYGLGERVIYVHDLPMPELLRHAKAVVTINSTAGISALIHN
                     KPLKVMGNALYDIKGLTYQGHLHQFWQADFKPDMKLFKKFRGYLLVKTQVNAVYYGGV
                     KFENALGMNKDLLFGSKSIS"
     gene            complement(64265..66292)
                     /locus_tag="PN977_000687"
     CDS             complement(64265..66292)
                     /locus_tag="PN977_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000579243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide biosynthesis protein"
                     /protein_id="HDI6040754.1"
                     /translation="MIGIYSPGIWRIPHLEKFLAQPCQKLSLLRPVPQEVDAIAVWGH
                     RPSAAKPVAIAKAAGKPVIRLEDGFVRSLDLGVNGEPPLSLVVDDCGIYYDASKPSAL
                     EKLVQDKAGNAALADQAREAMHTIVTGDLSKYNLAPAFVADESERADIILVVDQTFND
                     MSVTYGNAGPHEFAAMLEAAMAENPQAEIWVKVHPDVLEGKKTGYFADLRATQRVRLI
                     AENVSPQSLLRHVSQVYVVTSQYGFEALLAGKPVTCFGQPWYAGWGLTDDRHPQSALL
                     SARRGSATLEELFAAAYLRYCRYIDPQTGEVSDLFTVLQWLQLQRRHLQQRDGYLWAL
                     GLTLWKSAILKPFLQTATNRLSFSRRCTAASACVVWGVKGEQQWRAEAQRKSLPLWRM
                     EDGFLRSSGLGSDLLPPLSLVLDKRGIYYDATRPSDLEVLLNHSQLTLAQQMRAEKLR
                     QRLVESKLSKYNLGADFSLPAEAKDKKVILVPGQVEDDASIKTGTVSIKSNLELLRTV
                     RERNPHAYIIYKPHPDVLVGNRKGNIPAELIAELADYQALDADIIQCIQRADEVHTMT
                     SLSGFEALLHGKQVHCYGLPFYASWGLTVDEHHCPRRERRLTIADLIYQALIVYPTYI
                     HPTRLQPITVEEAAEYLIKTPRKPMFITRKKAGRVIRYYRKLIMFCKVRFG"
     gene            complement(66289..67029)
                     /gene="kdsB"
                     /locus_tag="PN977_000688"
     CDS             complement(66289..67029)
                     /gene="kdsB"
                     /locus_tag="PN977_000688"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001578967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HDI6040755.1"
                     /translation="MSKAVIVIPARYGSSRLPGKPLLDIVGKPMIQHVYERALQVAGV
                     AEVWVATDDPRVEKAVQAFGGKAIMTRNDHESGTDRLVEVMHKVEADIYINLQGDEPM
                     IRPRDVETLLQGMRDDPALPVATLCHAISAEEATEPSTVKVVVNTRQDALYFSRSPIP
                     YPRNAEKARYLKHVGIYAYRRDVLQNYSQLPESMPEQAESLEQLRLMSAGINIRTFEV
                     AATGPGVDTPACLEKVRALMAQELAENA"
     gene            complement(67039..68715)
                     /locus_tag="PN977_000689"
     CDS             complement(67039..68715)
                     /locus_tag="PN977_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016159830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysialic acid transporter KpsD"
                     /protein_id="HDI6040756.1"
                     /translation="MKLFKSILLIAACHAAQASAAIDINADPNLTGAAPLTGILNGQQ
                     SDTQNMSGFDNTPPPAPPVVMSRMFGAQLFNGTSADSGATVGFNPDYILNPGDSIQVR
                     LWGAFTFDGALQVDPKGNIFLPNVGPVKVAGVSNSQLNALVTSKVKEVYQSNVNVYAS
                     LLQAQPVKVYVTGFVRNPGLYGGVTSDSLLNYLIKAGGVDPERGSYVDIVVKRGNRVR
                     SNVNLYDFLLNGKLGLSQFADGDTIIVGPRQHTFSVQGDVFNSYDFEFRESSIPVTEA
                     LSWARPKPGATHITIMRKQGLQKRSEYYPISSAPGRMLQNGDTLIVSTDRYAGTIQVR
                     VEGAHSGEHAMVLPYGSTMRAVLEKVRPNSMSQMNAVQLYRPSVAQRQKEMLNLSLQK
                     LEEASLSAQSSTKEEASLRMQEAQLISRFVAKARTVVPKGEVILNESNIDSVLLEDGD
                     VINIPEKTSLVMVHGEVLFPNAVSWQKGMTTEDYIEKCGGLTQKSGNARIIVIRQNGA
                     AVNAEDVDSLKPGDEIMVLPKYESKNIEVTRGISTILYQLAVGAKVILSL"
     gene            complement(68739..69887)
                     /locus_tag="PN977_000690"
     CDS             complement(68739..69887)
                     /locus_tag="PN977_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000905920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule polysaccharide export inner-membrane
                     protein KpsE"
                     /protein_id="HDI6040757.1"
                     /translation="MLIKVKSAVSWMRARLSAISLADIQKHLAKIIILAPMAVLLIYL
                     AIFSQPRYMSESKVAIKRSDDLNSGSLNFGLLLGASNPSSAEDALYLKEYINSPDMLA
                     ALDKQLNFREAFSHSGLDFLNHLSKDETAEGFLKYYKDRINVSYDDKTGLLNIQTQGF
                     SPEFALKFNQTVLKESERFINEMSHRIARDQLAFAETEMEKARQRLDASKAELLSYQD
                     NNNVLDPQAQAQAASTLVNTLMGQKIQMEADLRNLLTYLREDAPQVVSARNAIQSLQA
                     QIDEEKSKITAPQGDKLNRMAVDFEEIKSKVEFNTELYKLTLTSIEKTRVEAARKLKV
                     LSVISSPQLPQESSFPNIPYLIACWLLVCCLLFGTLKLLLAVIEDHRD"
     gene            complement(69959..70942)
                     /locus_tag="PN977_000691"
     CDS             complement(69959..70942)
                     /locus_tag="PN977_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001327875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KpsF/GutQ family sugar-phosphate isomerase"
                     /protein_id="HDI6040758.1"
                     /translation="MSERHLPDDQSSTIDPYLITSVRQTLAEQGAALQNLSKQLDSGQ
                     YQRVLNLIMNCKGHVILSGMGKSGHVGRKMSATLASTGTPSFFIHPAEAFHGDLGMIT
                     PYDLLILISASGETDEILKLVPSLKNFGNRIIAITNNGNSTLAKNADAVLELHMANET
                     CPNNLAPTTSTTLTMAIGDALAIAMIHQRKFMPNDFARYHPGGSLGRRLLTRVADVMQ
                     HDVPAVQLDASFKTVIQRITSGCQGMVMVEDAEGGLAGIITDGDLRRFMEKEDSLTSA
                     TAAQMMTREPLTLPEDTMIIEAEEKMQKHRVSTLLVTNKANKVTGLVRIFD"
     gene            complement(71741..71911)
                     /locus_tag="PN977_000692"
                     /pseudo
     CDS             complement(71741..71911)
                     /locus_tag="PN977_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003031967.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            complement(72254..73096)
                     /locus_tag="PN977_000693"
     CDS             complement(72254..73096)
                     /locus_tag="PN977_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
                     /protein_id="HDI6040759.1"
                     /translation="MSDITISRPEVVTGHTDVICSTNIRHILAVRKSTLLQIDTLIRQ
                     LAEISAMTESIGGKAAPGWAMKQDFRCGCWLMEKPETAMKAITRNLDREIWRDLMQRS
                     GMLSLMDAQARDTWYRSLEYDNFPEISEANILITFEQLHQNKDEVFERGVINVFRGLS
                     WNYKTNCPCKFGSKIIVNNLVRWDRWGFHLITGQQTDRLVDLERMLHLFSGKPIPDNR
                     ENITIRLDGHIQSVQGKERYEDEMFIIKYFKKGSAHITFKRLELIDRINDIIARYFPS
                     VLSA"
BASE COUNT        18659 a        17061 c        18837 g        18574 t
ORIGIN      
        1 caggcgacca aagttctctt ctgcgcttcc tggaggtggc ccgtaattgt tggctaaatc
       61 aaagtgcgta atgcccaaat caaacgcttt acgcaggatc gcacgctgtg attccagcgc
      121 gttaacgtga ccgaaattgt gccataaacc gagcgataac gcgggcaggc gtaaaccgct
      181 ttttccgcaa tagcggtact gcatctgccc gtaacgttcg ggattcgcta accagaccat
      241 gacctctcct ttccaccgtt caatttcgaa acaatgtttc tagtttagcg attcgccagc
      301 gcgtatcccg tagtctggct cacagagtga cgaaaaactg ggcgaaaaca cgtgcttatg
      361 ctttgcttaa aaaaacacca gttgaggagt gcaacgatgc cgcgtttaac cgccaaagat
      421 ttcccacaag agttgttgga ttactacgac tattacgctc acgggaaaat ctcgaaacgt
      481 gagttcctca accttgcggc gaagtatgcg gtaggcggga tgacggcatt agcgttgttt
      541 gatttgctca agccaaatta tgcgctggcg actcaggtag agtttaccga cccggagatt
      601 gttgctgagt acatcacgta tccttcgcca aatggtcacg gcgaggtacg gggttatctg
      661 gtgaaacccg caaaaatgag cggcaaaacg ccagccgtgg tggtggtgca tgagaatcgt
      721 ggactgaatc cgtatatcga agatgtggca cggcgagtgg cgaaggcggg gtatatcgcc
      781 ctggcacctg acggcttaag ttccgttgga ggttatccgg gaaatgatga taaaggtcgt
      841 gagctgcaac agcaggttga tccaaccaaa ctgatgaatg atttctttgc cgcaattgag
      901 tttatgcaac gctatccgca agcgacaggc aaagtgggta ttaccggatt ttgctatggc
      961 ggtggcgtat cgaacgcggc ggctgtcgcg tatccggaac tggcctgcgc ggtgccgttt
     1021 tatggtcgcc aggcacccac tgccgatgtg gcgaagattg aagcgccttt actactccac
     1081 ttcgcggaac tggacacccg aatcaacgag ggctggcctg cttacgaggc ggcgttgaaa
     1141 gccaataata aggtttatga ggcgtatatc tatccggggg ttaatcacgg attccataat
     1201 gattccacgc cccgttatga caaatctgcc gccgatcttg cctggcaaag gacactgaaa
     1261 tggttcgata aatatctctc ctgataggtt tatctcttac gggattacgt cttaaacaag
     1321 catgaaaaaa tagcgtgcgc aaaagtcgtt ctttgcctaa aatatcgcta tatatgacaa
     1381 tatatagcga atgaggtgaa cgatgaataa ccattttggt aaaggcttaa tggcgggatt
     1441 aaaagcaacg catgccgaca gtgcggttaa tgtgacaaaa tactgtgccg attataaacg
     1501 cggttttgta ttaggctact cgcaccggat gtacgaaaag accggagatc gccagctcag
     1561 cgcctgggaa gcgggtattc tgacgcgccg ctatggactg gataaagaga tggtaatgga
     1621 tttctttcgt gagaataatt cctgttctac gttgcgcttt tttatggccg gttatcgcct
     1681 cgaaaattga tcaaacatac gtattatctt gctttaatta attacactaa tgcttcttcc
     1741 cttcgtttta gcgccccgcc gcagtatcat gatatcgata accataataa atgtgtggta
     1801 aatggcgcat cgatcgcatt attgattttg cgattgaggc aaaatatatg ccaggtcttc
     1861 gcaacggaat aactataaat gactggagat aacaccctca tccattctca cggcattaac
     1921 cgtcgtgatt tcatgaagct ttgtgcagca ttagccgcca ccatggggtt aagtagcaaa
     1981 gccgctgcag agatggccga atcggttact aacccgcagc gtccgccagt tatctggatt
     2041 ggcgcgcagg agtgcaccgg ttgtacggaa tctctgcttc gtgcaacgca tccaacggta
     2101 gaaaacctcg tgctggagac tatctctctg gagtatcacg aagtgctttc cgccgccttc
     2161 ggtcatcagg tcgaagagaa caaacataac gcacttgaga agtacaaagg gcagtatgtg
     2221 ttggttgtgg atggttccat cccattaaaa gataacggta tttattgcat ggttgctggt
     2281 gagccgattg tggatcacat ccgcaaagcg gcagaaggcg cagcagcgat tatcgctatc
     2341 ggttcctgct ctgcgtgggg cggtgttgcc gcagctggag ttaacccaac tggcgcagtc
     2401 agcctgcaag aagttctgcc aggcaaaacc gttatcaata ttccgggctg cccgccgaac
     2461 ccgcacaact tcctcgcgac cgttgcgcac atcatcactt acggcaaacc gccgaaactg
     2521 gatgacaaaa atcgtccgac cttcgcctat ggccgtctga ttcacgaaca ctgcgaacgt
     2581 cgcccgcact tcgatgctgg tcgttttgcc aaagagttcg gtgatgaagg ccaccgcgaa
     2641 ggctggtgcc tgtaccacct cggctgtaaa gggccagaaa cttacggcaa ctgctcaacg
     2701 ctgcaattct gcgatgttgg cggtgtgtgg ccggtggcga ttggtcaccc gtgctatggc
     2761 tgtaacgaag aaggtatcgg cttccataaa ggcatccatc agcttgccaa cgtcgaaaac
     2821 cagactccgc gttcacagaa accggatgtt aacgctaaag aaggcggcaa cgtctcggca
     2881 ggcgctattg gtttgctcgg cggtgtggtt gggctggttg ccggtgtcag cgtgatggcg
     2941 gttcgtgaac tgggtcgtca gcaaaagaaa gataacgctg actcacgggg agaataaccg
     3001 tgaacagacg taattttatt aaagcagcct cctgcggggc attgctgacg ggcgcgctgc
     3061 cgtctgtcag tcatgcggct gctgaaaacc gcccgccaat tccgggatcg ctggggatgt
     3121 tgtacgactc gaccttgtgc gttggctgcc aggcgtgcgt caccaagtgt caggatatca
     3181 acttccctga acgtaacccg caaggggaac agacctggtc gaacaacgac aaactgtcgc
     3241 cgtacaccaa taacatcatt caggtgtgga ccagcggcac aggggtcaac aaagaccagg
     3301 aggagaatgg ctacgcgtac attaagaaac agtgtatgca ttgcgtcgat ccgaactgtg
     3361 tctctgtgtg cccggtctct gcactaaaaa aagatccgaa aaccggcatt gtccattacg
     3421 acaaagatgt gtgcaccggc tgccgttact gcatggtcgc ctgcccatac aatgtgccga
     3481 agtacgacta caacaacccg tttggtgcgc tgcataagtg cgagctgtgc aaccagaaag
     3541 gtgtggaacg tctcgataaa ggcggtctgc ctggctgcgt agaagtgtgc ccggcgggcg
     3601 cggtgatttt cggtacgcgt gaagagctga tggcggaggc gaaaaaacgt ctggcgctga
     3661 agcctggcag cgaataccac tatccgcgtc agacgctgaa atctggcgac acttacctgc
     3721 atacggtgcc gaaatattat ccgcatctgt acggcgagaa agagggcggc ggtactcagg
     3781 ttctggtact gacgggtgtg ccttatgaaa atctcgacct gccgaaactg gacgatcttt
     3841 ctaccggtgc gcgttccgaa aatattcaac acaccctgta taaaggcatg atgctaccac
     3901 tggctgtgct ggcgggctta accgtgctgg ttcgtcgcaa caccaaaaac gaccatcacg
     3961 acggaggaga cgatcatgag tcatgatcca caaccgctgg gcggcaaaat catcagtaaa
     4021 ccggtcatga tttttggacc gttaatcgtc atctgtatgc tcctgattgt gaagcgtctg
     4081 gtgttcggtc tgggctcagt ctctgacctg aacggcggct tcccgtgggg cgtgtggatc
     4141 gcgtttgacc tgctgatcgg caccggcttt gcctgtggcg gctgggcgct ggcgtgggcg
     4201 gtatacgtct ttaaccgtgg gcaataccat ccgctggtgc gtccggcgct gttggcgagt
     4261 ctgtttggtt actcactggg tggcttgtcg atcactattg acgtgggtcg ctactggaac
     4321 ctgccgtact tctacattcc gggtcacttc aacgtgaact cggtactgtt cgagacggcg
     4381 gtctgtatga ccatctacat cggcgtgatg gcactggagt ttgctccagc actgtttgaa
     4441 cgtctgggct ggaaggtgtc gctacagcgt ctgaacaagg ttatgttctt catcattgca
     4501 cttggcgcgc tgctgccgac catgcaccag tcttcaatgg ggtcgctgat gatctcggcg
     4561 ggctacaagg tgcatccgtt gtggcagagc tatgaaatgt tgccgttgtt ctcgctgctg
     4621 acggcgttca tcatgggctt ctcgattgtc atctttgaag gttcgctggt gcaggcgggt
     4681 ctgcgtggca acggtccgga tgaaaagagt ctgttcgtta agctgaccaa caccatcagt
     4741 gtgttgctgg cgattttcat cgtgctgcgc tttggcgagc ttatctatcg cgacaagctg
     4801 tcgttagcgt ttgccggtga cttctattcc gtgatgttct ggattgaagt cctgctgatg
     4861 ctcttcccgc tggtcgttct gcgtgtggcg aagctgcgta atgattcccg catgctgttc
     4921 ctgtcagcac tgagcgcgct gttaggttgt gcaacctggc gtctgaccta ttcgctggtg
     4981 gcattcaacc cgggtggcgg ttacgcctac ttcccgacct gggaagaact gttgatttct
     5041 attggttttg tggctattga gatttgcgct tacatcgtac tcattcgtct actgccgata
     5101 cttcctcctt taaaacaaaa cgatcataat cgtcatgagg cgagcaaagc atgagccaga
     5161 gaattactat tgatccggta acccgtattg aagggcattt acgcatcgat tgcgaaatcg
     5221 aaaatggcgt cgtttcgaaa gcatgggctt ccggtaccat gtggcgcggc atggaagaga
     5281 tcgtgaaaaa ccgcgatccg cgcgatgcat ggatgattgt gcaacgtatc tgtggcgtat
     5341 gtactaccac tcacgcgctg tcttccgttc gtgcggcaga aagtgcactg aatatcgacg
     5401 ttccggttaa cgcgcaatac atccgtaaca tcattctggc tgcgcacacc acgcatgacc
     5461 atatcgttca tttctatcag ctctcggcgc tggactgggt ggacatcact tctgcactgc
     5521 aagctgaccc aaccaaagcc tccgaaatgc tgaaaggtgt ttcgacctgg cacctgaaca
     5581 gcccggaaga gttcaccaaa gttcagaaca agattaaaga tctggttgcc agcggtcagt
     5641 tggggatttt cgccaatggc tactggggtc acccggcgat gaaactgccg ccagaagtga
     5701 acctgattgc ggtagcgcac tacctgcagg cgctggagtg ccagcgtgac gctaaccgcg
     5761 tcgtggcgct gctgggcggt aaaacgccac acattcagaa cctggcggta ggtggggtcg
     5821 ctaacccaat caacctcgac ggtctgggcg tgctgaacct tgagcgcctg atgtacatca
     5881 agtctttcat cgataaactg agcgactttg ttgagcaggt ttataaggtt gataccgcgg
     5941 ttatcgccgc gttctacccg gaatggctga cgcgcggtaa aggtgcggtg aactacctga
     6001 gcgtgccgga attcccgacc gacagcaaaa acggcagctt cctgttcccg ggcggctaca
     6061 ttgagaatgc ggatctgtcc tcgtatcgcc cgatcacttc ccactccgat gaatacctga
     6121 ttaaaggcat tcaggagagc gcgaagcact cctggtataa agacgaagcg ccgcaggcac
     6181 cgtgggaagg taccaccatt ccggcttatg atggttggtc tgacgacggt aaatattcct
     6241 gggtgaaatc accgactttc tacggcaaaa cggtagaagt gggtccgctg gctaacatgc
     6301 tggtgaaact ggcggcagga cgcgagtcta cccagaacaa actgaatgaa atcgttgcga
     6361 tttatcagaa actgactggc aacacgctgg aagtggcgca gctgcactcc acgctgggcc
     6421 gtattattgg tcgtaccgtt cactgctgtg aattgcagga tatcctgcaa aatcaataca
     6481 gtgcactgat caccaatatc ggcaaaggcg atcacaccac ctttgtgaag ccgaacattc
     6541 cggcaacggg tgagttcaaa ggcgtaggtt tcctcgaagc accgcgcggt atgctctctc
     6601 actggatggt tattaaagac ggtatcatca gcaactacca ggcggttgtt ccatcaacct
     6661 ggaactctgg tccgcgtaac ttcaatgatg acgtcggtcc ttacgagcag tcgctggtgg
     6721 gtacgccggt tgccgatccg aataaaccgc tggaagtggt gcgtaccatt cactccttcg
     6781 acccgtgcat ggcctgtgcg gtacacgtag tggatgctga cggcaacgaa gtggtttcag
     6841 tgaaggttct gtaatgcgta ttttagtctt aggggtcggc aatattttgc tgaccgatga
     6901 agccatcggc gtgcggattg tcgaagcgtt agagcaacga tacattctgc cggattatgt
     6961 tgagatcctc gatggcggca cggcgggaat ggagctgctt ggcgacatgg caaatcgcga
     7021 tcatctgatt attgcggatg ccattgtgtc gaaaaagaac gcgccgggaa cgatgatgat
     7081 cctgcgggat gaagaagttc cggcgttgtt taccaacaaa atctctccgc atcagcttgg
     7141 cctggccgac gtattgtcgg ccctgcgctt caccggcgag tttccgaaaa agctgaccct
     7201 ggtcggcgtg atcccggaat cactggagcc acacatcggc ttaacgccga cggtcgaagc
     7261 gatgattgaa cctgcgcttg agcaggttct ggctgcgctg cgcgaatcag gcgtggaagc
     7321 tatcccacgg gaggcgattc atgactgaag agatagcagg tttccagacc tccccgaagg
     7381 cgcaagtaca ggcagcgttt gaagaaattg cccggcgttc gatgcacgat ctctcttttc
     7441 tgcatccttc aatgccggtg tatgtttctg actttacgct gttcgaaggt cagtggacgg
     7501 ggtgtgtgat caccccgtgg atgctgagtg cagttatctt ccccggcccg gatcaactct
     7561 ggccgctgcg caaagtgagt gaaaaaattg gtctgcaact gccgtatggc actatgacct
     7621 ttaccgttgg cgaactggac agtgtttcgc aatacctctc ctgctcgctg atgtcgccgc
     7681 tttcgcacag catgtcgatt gaagagggcc aacggctgac ggatgactgc gcccgaatga
     7741 tcctttcgct gccagtcacc aatccggatg taccacacgc agggcgtcgt gccctgctat
     7801 ttggccgcag gagtggcgaa aatgcatgag ttgtcgcttt gccagagcgc cgttgaaatt
     7861 atccaacggc aggcggagca gcacgatgtt aagcgcgtca ccgccgtgtg gctggaaatt
     7921 ggtgcgctct cctgcgttga ggagagtgcc gtccgtttta gttttgaaat tgtctgccac
     7981 ggaacggtgg cgcaagggtg cgatttacat atcgtctata aacccgccca ggcctggtgc
     8041 tgggattgca gccaggtggt ggagattcat cagcacgatg cgcagtgtcc gatctgtcat
     8101 ggcgagcggt tgcgtgtcga taccggcgat tcgctgatcg tcaaaagtat tgaagttgaa
     8161 taaccggagt taacaatgtg tattggcgtt ccaggccagg tgctggctgt cggtgaagat
     8221 attcaccagc ttgcgcaggt tgaagtatgt ggtatcaagc gcgacgtgaa tatcgccctg
     8281 atttgtgaag gtaaccctgc cgatctgctg ggccagtggg tactggtaca cgtcggattt
     8341 gccatgagca ttatcgatga agacgaagcc aaagccacat tagacgcact gcgccaaatg
     8401 gattacgaca ttaccagcgc gtgatgatta aagccggatg cggcgtaaat gctttatccg
     8461 gctacatagc gataagcctg ccaatgttgc gacttacccc tgacgcttat cttccgtatt
     8521 cgtctcgaaa tcactggcgt catggcgctc atgcaactgc tcgttcagcg gcccgttggt
     8581 gcggttaaca atacgcccac gcttcaccgc cggacgttcg cccacttctt tcgcccagcg
     8641 ttgtacgtgt ttataacttc ccgcatcaag aaactctgcg gcatcataca cgccgcctaa
     8701 caccacgttg ccaaaccacg gccagatcgc catatccgca atggtgtact catcgcccgc
     8761 aacaaacttg tgctgcgcca gttgcttatc cagcacgtcg agcaggcgct tggcttccat
     8821 ggtaaagcgg ttgatggcgt actcaatttt caccggcgcg taatggtaaa agtgaccaaa
     8881 accaccgccg aggaacggtg ccgcgccctg taaccagaac agccagttca gcgtttcagt
     8941 ccgttttgcc agatcctgcg gcaggaagta gccaaatttc tccgccagat aaagcagaat
     9001 cgatccagac tcaaacacgc ggatcggtgg attatgggta tggtcgcgca atgccgggat
     9061 cttcgagttt gggttcactt cgacaaagcc gctggagaat tgatcgccat cgccaatacg
     9121 aatcagccag gcgtcgtact ctgcaccagt aacgcccagc gccagcagct cctcaagcat
     9181 aatcgttact ttctgtccgt ttggcgttcc cagcgaatag agttgcagcg gatgtttgcc
     9241 caccggcagc gttttttcat gcgtcggacc agaaaccggg cgattgatat tggcgaacgc
     9301 gccgccagcg gatttatccc acgtccagac tttcgcgggc tgataagtat tgtctgtcat
     9361 agtgagttgc cttctgagtg gtagtgttga agcagtgtag caggtcactt gctgtacaga
     9421 atgtgaattt tttaaccctt tgcgccagcc gctgcgttga ggtgtatgat aagtcagacc
     9481 tctgtccggt taatgtcgct gagcaggtaa tcagagcaca acaattacgc caactgctta
     9541 ggttgtattg ttaaaggtaa agtgatgagc aaaggaacga ccagccagga tgccccgttc
     9601 gggacattat tgggctacgc cccaggtggg gtagcaatct actcttcaga ttacagttct
     9661 ctcgatccgc aggaatacga agatgacgcc gtattccgta gctatatcga cgacgaatat
     9721 atgggccaca aatggcaatg cgttgaattt gctcgccgtt ttctctttct gaattacggt
     9781 gtggtcttca ctgacgtggg tatggcgtgg gagattttct cgctgcgctt cctgcgtgaa
     9841 gtggttaatg acaacatcct gccattgcag gcatttccta atggctcgcc gcgtgcaccg
     9901 gtcgcgggcg cgcttcttat ctgggataaa ggcggtgaat ttaaagacac tggccatgtc
     9961 gccatcatta ctcaattgca tggcaacaaa gtccgtattg cggaacagaa cgtgattcat
    10021 tccccgttgc cgcaagggca acagtggaca cgcgagctgg agatggtggt cgaaaacggc
    10081 tgctataccc ttaaagacac gtttgatgac accaccattc tgggctggat gatccagacg
    10141 gaagataccg actacagctt accgcagccg gaaattgcag gcgagctgct gaaaatcagc
    10201 ggagcgcgtc tggaaaacaa aggtcagttt gacggtaaat ggctggatga aaaagatccg
    10261 ctgcaaaacg cctatgtaca ggccaacggc caggtgatca atcaggatcc gtatcattac
    10321 tacaccatta ccgagagtgc cgagcaggag ctgattaaag ccaccaacga gctgcacctg
    10381 atgtatcttc acgcaaccga caaggtgcta aaagatgaca acctgctggc gctgttcgac
    10441 attccgaaaa tcctctggcc acgtttgcgt ctctcctggc agcgtcgccg tcaccatatg
    10501 atcaccggtc gtatggattt ctgcatggac gaacgtggcc tgaaggtcta cgaatacaac
    10561 gccgattccg cctcctgtca taccgaagcg ggcttgatcc tcgaacgttg ggcggagcag
    10621 ggctataaag gcaacggctt caatcctgcg gaagggctga ttaacgaact ggctggtgcc
    10681 tggaaacaca gtcgtgcacg tccgtttgtc catatcatgc aggacaaaga tatcgaggaa
    10741 aactatcacg cgcagtttat ggagcaggcg ctgcaccagg cgggctttga aacgcgtatc
    10801 ttgcgtgggt tggatgaact gggctgggat gctgccgggc aactgattga tggggaaggg
    10861 cgactggtta actgcgtgtg gaaaacctgg gcgtgggaaa ccgcgtttga tcagattcgt
    10921 gaagtcagcg accgtgagtt tgctgcggtg ccgatccgta ccggtcatcc gcaaaatgaa
    10981 gtgcgtctta tcgatgtatt gctgcgcccg gaagtgctgg tctttgaacc tctgtggact
    11041 gtgatccccg gcaacaaagc gattctgccg atcctctggt cgctgttccc gcaccaccgc
    11101 tatctgttgg atactgattt caccgttaat gatgaactgg tgaaaaccgg ttatgcagtg
    11161 aaaccgatcg ccggtcgctg tggtagcaat atcgacctgg tcagccatca tgaagaggtg
    11221 ctggacaaaa ccagcggtaa atttgccgag cagaaaaaca tctatcagca actgtggtgt
    11281 ttgccgaaag tggacggtaa atacattcag gtatgtacct tcaccgttgg cggcaactac
    11341 ggtgggacgt gtttgcgcgg tgatgaatca ctggtcatca aaaaagagag tgatattgaa
    11401 ccgttaattg tggtgaaaaa gtaatacatg ttgtggcaac cccgtactga ttatgaagaa
    11461 taatcagtac ggggattaat caaaacattc aatatttata tattccaata tttattcatt
    11521 ttatatgtga atatattaac caacagaata cctgtgtttt gattatttct aaaggttttg
    11581 aatgaatgct tattgtctga tacacaagaa ataacacgct ttttatcgtt aaaaaatgat
    11641 atttcacttt gcccatgccg ttaaagtcac tgataatgcg tccgttcgta aattcaaaat
    11701 ggcgtaatct aatatatgct aaatttattt gttggccttg atatatacac agggcttttg
    11761 ttattacttg ctctggcatt tgtgttgttc tacgaagcaa tcaatggttt tcatgacacg
    11821 gcgaatgcgg tggcaaccgt tatttatact cgtgccatgc aaccacaact tgctgtggtg
    11881 atggcggcat tttttaattt ttttggcgtg ttattgggcg gacttagcgt tgcctatgcc
    11941 attgtccata tgttgccaac cgatttgttg ctgaatatgg ggtcaaccca cggtctggcg
    12001 atggtctttt ccatgctgct ggcggcgatt atctggaacc tgggaacgtg gttctttggt
    12061 ttaccggcct ccagttcgca caccttgatt ggtgcgatta tcggcatcgg tttaaccaac
    12121 gcgctgttaa ccggctcatc ggtgatggat gcgttaaacc tgcgtgaagt gaccaaaatt
    12181 ttctcctcgc tgattgtttc ccctatcgtc ggcctggtca ttgcgggtgg tctgattttc
    12241 ctgctgcgcc gtttctggag cgggacgaaa aagcgtgacc gtattcaccg cattccggaa
    12301 gatcgcaaaa agaaaaaagg caaacgtaag ccgccattct ggacgcgtat tgcgctgatt
    12361 gtttccgctg cgggcgtggc gttttcgcac ggcgcgaacg acgggcagaa agggatcggc
    12421 ctggtgatgc tggtactggt ggggattgcc cctgctggct tcgtcgtcaa tatgaacgcg
    12481 tccggctatg aaattacccg tacccgcgat gccgtcacca acttcgaaca ctacctgcaa
    12541 cagcatcctg aactgccgca gaagttgatt acgatggaac ctccattgcc tgcgacatcg
    12601 actgatggca cgcaagtaac agagtttcac tgtcatccgg caaatacctt tgatgcgatt
    12661 gcgcgcgtta aaacgatgct gccaggcaat atggaaagtt acgagccgtt aagcgtgagt
    12721 cagcgcagcc agctgcgccg cattatgctg tgcatctctg atacctccgc gaagctggcg
    12781 aaactgccag gcgtcagtaa agaagaccag aacctgctga aaaaactgcg cagcgatatg
    12841 ttaagcacca ttgagtacgc tccggtgtgg atcatcatgg cggtagcact ggcgctcggc
    12901 attggcacca tgattggctg gcgtcgtgta gcgatgacca tcggtgagaa gattggtaag
    12961 cgcggcatga cgtatgcgca aggcatggcg gcacaaatga cggcggcagt gtctatcggc
    13021 cttgccagtt atattgggat gcccgtctcc acaacacacg tactctcatc tgccgtagca
    13081 ggaacgatgg tggtggacgg cggtgggtta cagcgtaaaa cggtaaccag catcctgatg
    13141 gcgtgggtgt ttactttacc ggcggcgatt tttctttctg gtgggttgta ctggatagca
    13201 ttgcagttga tttaaccttc ctgaaaatgc ccggtcctgg cgatcgggca tttccatttt
    13261 tcactagtga taaccacgcg cggtcataaa atccattatc gctttttccg catcaactag
    13321 cacttgttcc agcggttgat tggcattgat atcaaccagt tgcgcgcctc caaaggttaa
    13381 ctgtggcgtt atggcaatct tcctcgccag cgattcccgt ttatggtcgg gtttacgcgc
    13441 gcaggcaact tcaaggtcaa cattgagttt gatgaccaga tcaggtttat ggctcgccat
    13501 ccagtgaaac gctttacgct cctggcttgc cagccatgag acaaaacgac caccttcaac
    13561 gttaggcgga aacaccgtac catcgtaagc gccaggaatt tggtcctgag gataacggtc
    13621 ggttagaaca attaacccgc gacgacgaca ggcaagcata tgacgaaagc gcagtaagcg
    13681 acgggcgaca aacgctgtaa ttaccagcgc cggaacaggg ccaggcaatt tttttgctgt
    13741 tttcacctga tttcgttcaa ttgttttatg taaggatttt cccatcaacg gtaattttgt
    13801 cactgcacga ccgacatttc cggcctgttt tcctaaatga actctttcgg ctgcaccata
    13861 tttttcgaca acggtaatga gatgttcaca caccgttgac ttgcctgaac catcgctacc
    13921 aataacggca attaatggag gtgttattga ctgcatgaat atatccttta atttaaatat
    13981 ccattaaaaa tatttatttg gttaatatgt ttttatgaaa gcgtaattca ggtcaatgtc
    14041 acaattaacc attgtcacaa taaggttgaa cggatattct gagcacggac cacgtaatat
    14101 tcaatacatt atttgctgcc gggtttttcg tgaatcaacg taatatgtca ataattaatt
    14161 ccacgccagt gcgtgttatt gccattgtag gatgtgatgg ttcaggtaaa tcgaccctca
    14221 cggcaagcct ggtaaatgaa ctggcagcaa caatgccaac agaacacatt tatctcgggc
    14281 aatcgtccgg gcgaattggc gaatggattt cacagctccc tgttattggc gcaccttttg
    14341 ggcgttatct gcgaagtaaa gcggcacatg tgcacgaaaa gcccttaaca ccgcctggca
    14401 atattactgc gctggttatc tatctgcttt cctgctggcg ggcgtacaag tttcgcaaaa
    14461 tgttgtgtaa aagccagcaa ggctatctgc tcatcaccga ccgctacccg caggttgaag
    14521 tgccgggttt tcgctttgat ggcccgcaat tggcaaaaac cacgggcggt aacggttgga
    14581 taaaaatgtt aaggcagcgc gagctgaagc tgtaccaatg gatggcgtct tatttgcccg
    14641 tattgttgat tcgtcttggc attgatgaac aaaccgcgtt tgcgcgtaaa cctgaccacc
    14701 aactggcagc gttacaggaa aaaatcgccg ttacgccgca actgacattc aatggcgcaa
    14761 agatccttga actggatggt cggcaaccag ccgatgaaat tctgcaagcg tcactacgcg
    14821 caattcacgc tgcgctttcc tgatcaccaa aatggatagg ggatgattta acgaatggac
    14881 gcactacaaa cacaaactgt taatagtaca accgcgccgc agcccaacta cattccgggg
    14941 ctgattgcgg tggtcgggtg tgatggcacc ggtaaatcca cactgaccac cgacctggtg
    15001 aaatcgctgc aacaacactg gcaaaccgag cggcgctatc tggggctgct ctcgggcgaa
    15061 gacggcgaca aaatcaaacg attgccgttg gttggcgtct ggctcgaaag gcgactggcg
    15121 gccaaatcat cgaaaaccca aagcatgaaa accaaatctc cagcgctatg ggcggcggtg
    15181 attatgtact gcttctcgct gcgaagaatg gcgaatctac gcaaggttca gcgactggcg
    15241 caaagtggcg ttctggtggt cagcgatcgc ttcccgcagg ctgaaatttc gggcttttat
    15301 tatgacggac cggggatcgg cgtcgaacgt gcgaccggga aaatcagcat gtttctggcg
    15361 cagcgcgaac ggcgtttata ccaacaaatg gcgcaatacc gtcctgaatt aatcattcgt
    15421 ttggggattg atattgatac tgccatttcc cgcaagcctg accatgacta tgccgaattg
    15481 caggacaaaa tcggcgtcat gtcgacgatt ggctataacg gcacaaaaat tcttgagatt
    15541 gattccagag cgccttacag cgaagtgctg gaacaggcac agaaggcggt ttctctggtt
    15601 gccatcgttt ctgaccgccg aagtttaact taaggctggc gggatacttt ttactatcaa
    15661 cgataaggtc aggctgaatt ggcgggtttt aacatcaaac attggtttgc agatggcgcg
    15721 tttcgcacca ttattcgcaa tagcgcctgg ttaggctcca gtaatgtcgt gagcgcttta
    15781 ctgggtctgt tggcgctctc gtgtgccggt aaagggatga cgcccgccat gtttggcgta
    15841 ctggtgattg tgcaatcgta cgccaagtcg atcagcgatt ttattaagtt tcagacatgg
    15901 cagttggtgg tgcagtacgg aacgccagcc ttgaccaaca ataatccgca gcaatttcgc
    15961 aatgtcgtct cattttcctt ctcgctggat atcgtcagcg gcgcggtggc gattgtcggc
    16021 ggcattgcct tactcccttt tctttcccat tcattaggtc tggatgacca aagcttttgg
    16081 ctggcagcgc tgtattgcac gctcattcct tcaatggctt cctccacgcc gaccggcatt
    16141 ctgcgtgcgg tagatcgctt cgatctaatt gctgtacagc aagcgacgaa accttttctg
    16201 cgtgcagcgg ggagcgtcgt agcctggtat tttgactttg gttttgcggg ttttgttatt
    16261 gcctggtacg tgtcgaatct ggttggcggc accatgtact ggtggtttgc cgcgcgcgaa
    16321 ttacgccgca gaaatatcca taacgccttc aaattgaatc tgtttgagtc tgcccgacac
    16381 attaaaggcg cgtggagttt tgtctggtca accaacattg cccactccat ctggtcggcg
    16441 cgtaactcgt gcagcacagt gctggtgggg atcgtgttag gacccgctgc cgccgggtta
    16501 tttaaaatcg ccatgacatt cttcgacgcc gccggaacgc cagcgggttt actgggcaaa
    16561 agtttttacc cagaggtgat gcgtttagat ccgcgcacca ccagaccgtg gttgttaggc
    16621 gtgaagtcag gtttactggc gggcggaatc ggtattctgg tggcgctcgc ggtgttgatt
    16681 gtcggcaagc cgctcatttc gctggtgttt ggcgttaagt atctcgaagc gtatgacctg
    16741 attcaggtga tgctgggcgc aattgtgatc tcgatgctgg gcttccctca ggagtcattg
    16801 ctattgatgg cgggaaaaca gcgcgctttt ctcgtggcgc aaaccatcgc ctcaatcggc
    16861 tacatcgtat tgctgttcat gttctgtcat ctgtttggcg tgctgggagc agcgtttgct
    16921 tatttcggcg gtcaatgtct ggatgtggtg ctctcgctaa ttccaacctt aagggcattt
    16981 tttcagcgcc attccttgct ttataacgca gccggagaga aatcctgatg atgagaaaat
    17041 atttcccgct tgaggctagc gagcgtctat ttgttgcgat tgaagaagat gatgtcgttg
    17101 atgcacaggt cagtttgccg ccgactatcg cactaagttg taccaccgaa atcatccatg
    17161 ataattacgc gttatgcctg caattctggc tcaatggcgt caatcgccag gagttactgc
    17221 gtttaatatg caaacaggcc aaaggcgatg agctgactgc tgatgagcgc aaacaattta
    17281 aatacatgcg agcgcgctac aagcacctgc gttttgcgca acggctgtat ttaaagaagc
    17341 atcaggccgg atttttattc ggtaaaacga ccgttttttt ggggcgtttt caggacggct
    17401 ttcgtaacgg taagaaaaat attgtgtcgt attacggcaa cctgttacgg gtttatttaa
    17461 gctctccggt ctggtcgttg gtgaactatt cattacgcca tagccagtta gaaagcgtaa
    17521 gcggttttat tgcctatcgg caaaagcaaa tgcataccct gaaagagata attgccaaac
    17581 cgcgactcac cggcagggaa tttcatgatg tgcgaaaaat catcagccag caggtttctt
    17641 attacgacac cttaagatcg cttgatcctg aaaataaaga agcgttacag atttcccgct
    17701 tcctcgccgc cattaatggc ctgatggggg ataaacatga cgacatggtg gcggatgata
    17761 tggaaaaccg ccattcgtat gatgcaccga tggcgctgga tagcgatatt cgtcagcgct
    17821 tagaactgct gatttcgcgt tttcctctat aaccggagca atgatgtcat ctcgtttgat
    17881 tattgcgctg gttatcatgc tcttagcctc gggtgtgcag gcacacaatt tcgtcactgg
    17941 caaaacggtt acccctgttt atatccagga aggcggcgag ctgcttctga atagcgaaga
    18001 cgaaattcac taccaaaagt ggaacagcac ccaattggcg ggaaaagtcc gcattattca
    18061 gtatatcgcc gggcgtaagt cggcgaagaa aaagaactca ctgttaatta aagcggttga
    18121 agcggcaaac tttccgcagg atcgttttca gcccaccacc attgtgaaca ccgatgatgc
    18181 catctttggt actggctatt ttgtcgttgg caaaatcgaa aaaaataaac gccgctatcc
    18241 gtgggcgcaa ttcgttattg acggcaatgg actgggacgc gttgcctggc ggttgccgga
    18301 gcagagttca accattctgg tgctgaataa agacgggcaa attcagtggg caaaggacgg
    18361 cagcctgacg ccggaagagg tagatcatgt cattgcgctg gcgcagaaac tgatcaatga
    18421 ataaaaaatt caaaggattg agggtgttag tgagatgaag ctggttgaac actacatcat
    18481 gcgtggtacg cgccgtctgg tgctgattat cgtcggtttt ctgatcttta ttttcgccag
    18541 ctactccgca cagcgttatc tgaccgaagc ggcaaatggg accttagctc ttgatgttgt
    18601 gctggatatc gttttctaca aagtgctgat tgcactggag atgttgttac ctgttggtct
    18661 gtatgtgtca gttggcgtga cgctagggca gatgtatacc gactcggaaa ttaccgctat
    18721 ctctgcggcg gggggcagtc cgggacgctt gtacaaagcc gttctttatc tggcgatacc
    18781 gctaagtatt tttgtcaccc ttctgtcgat gtatggtcgg ccgtgggctt atgcgcagat
    18841 ttatcaactg gagcaacagt cacagtcgga gctggatgtt cgccagttgc gggcaaagaa
    18901 atttaacact aacgataacg gacgaatgat cctttcgcag acggttgatc aggataataa
    18961 tcgcctgact gacgcgctga tttatacttc tactgccaat cgaacccgca ttttccgcgc
    19021 ccgttcggtt gatgtggttg acccatcacc tgagaaaccg accgttatgt tgcataacgg
    19081 gaccgcctat cttctcgatc atcaggggcg tgacgacaac gaacagatct accgtaatct
    19141 gcaattacat ctgaatccgc tggatcaaag ccctaacgtc aaacgcaaag caaaatcggt
    19201 cacggagctg gcgcgctccg cctttcctgc cgatcatgcc gaactgcaat ggcgacaaag
    19261 ccgtggcctg acagcattgt tgatggcgct gctggccatt tcattaagtc gggtaaaacc
    19321 gcggcaaggg cgattttcaa cgttattgcc actgacgttg ctgtttgttg ccatttttta
    19381 tggcggcgac gtctgccgta cgctggtggc taacggtgcg attcccctca ttcctggttt
    19441 gtggttagta cccggactca tgctaatggg cctgctgatg ctggtcgcac gcgacttctc
    19501 tttgctgcag aaattttccc gatgaatgtt ttcagtcgct atttaatccg tcatctcttt
    19561 ctcggttttg ccgccgccgc agggctattg ctgccgcttt ttaccacctt caacctgatt
    19621 aacgaactgg atgatgtcag cccgggcggt tatcgctgga ctctggcggt gctggtggtg
    19681 ctgatgacct taccgcgtac actggtcgaa ctttcgccat ttatcgcatt attgggaggg
    19741 attgttggcc tggggcagtt atcgaaaaac agtgagctta ccgccattcg cagcactggg
    19801 ttttctatct tccgtattgc actggtggcg ctggttgccg ggatattgtg gactgtttcg
    19861 ttaggcgcga tagatgagtg ggtggcgtcg ccattgcagc agcaggcgct gcaaatcaaa
    19921 tcgactgcca ccgcgttggg ggaggacgat gacattaccg gcaatatgct ttgggccagg
    19981 cgcggtaatg aatttgtgac ggtgaaatcg ctgaacgagc agggccagcc tgtgggcgtg
    20041 gagatatttc attatcgcga cgatctctcg ctcgaatcct acatttttgc acgcagtgcc
    20101 tccattgaag acgacaaaac gtggatcctg catggtgtga atcataaaaa atggttgaat
    20161 ggcaaagaaa cgctggaaac atcagataat cttgcctggc aatcggcctt caccagtatg
    20221 gatcttgaag agttatcgat gccggggaat actttttctg tccgtcagct taatcattac
    20281 atccattatt tgcaggaaac cggacaaccc agcagcgaat accgccttgc actgtgggaa
    20341 aaactggggc aaccgatcct gaccctggcg atgattttgc tggctgtgcc gttcaccttt
    20401 agcgccccgc gctcgccagg ggtgggtagc cgtctcgctg taggcgtcat cgttggctta
    20461 ctcacctgga tcagctatca aatcatggtc aatctgggat tgttatttgc gttgagcgca
    20521 cctgttaccg cgctcggttt accggtagcg tttgtgttgg tggcgttgag cctggtgtat
    20581 tggtatgaca gacaacatta aacccgccgt ggcgcaacac cacagcgggc tcacattact
    20641 gcttcagagt agcaaatgcc tgaatgatgg catcgatttg ctcacgactg tgggcagcgt
    20701 ttacgctaca gcgcagcaga gtgatgcctg caggcgcggc aggtggcagg ataagattca
    20761 cataaacgcc cagcgaaata agctcgcgcc agatgcgtaa accttcctct ttcgaaccga
    20821 ttattactgg cactaccggg ctaatatgtg aacttaactc ataacccagt ttttgcagac
    20881 catcatacag atggttggcg ttatccatca gtttttgccg cagctctggt tgtgtggcaa
    20941 tggttcttaa cgacgagcgc acggtcgcaa tgcaggacgg agaaggagag gcggtaaaaa
    21001 tatacgggcg gctgccgtag cgcagcactt ccatcgcttc cgaaccgaca gcaaaaccgc
    21061 caatcgaggc cagactttta ctgaaggtgc caacgataat atccacatca tcttcaacgc
    21121 caacggcttc agccagtccg cgccccgttg cgcctaacac gccaaacgaa tgcgcttcat
    21181 cgacaatcaa ataaccaccg agacgacgtt tgatatcaac aatttccgct aacggcgcaa
    21241 cgtcaccgag catgctgtag atgccctcaa cgatgataat cgcctctttc gcccgttcac
    21301 cgaggcgaac catgcgccgt tccagatctt tagcatcgtt atggcggaaa cgaataattt
    21361 ccgcgccacc gagagaacag gcgtcataaa tactggcgtg gctgtcggca tccagtaaca
    21421 ccacggcgtt atggtcggca agtgcgctga tgacgcctaa attagcggta taaccggttg
    21481 aaaaaacgat agcggtaggg cgattgaaaa actcggcaat ctctttctct aacgccaggt
    21541 gcggcgcata actgccgttt gccatacgag aaccggtggt gccggtacct tgggccgcta
    21601 acgcggcctg accttcgctg atggcatcat gattaaaggt taaaccgaga taattattgg
    21661 tcccggccag aatcactttt ttattgccga tccggccttc ggtggcggaa tacacttcat
    21721 caatacaggt gccaaacggt gttaacccgt tgtcactaaa ttgcagtcgc tcgccagcaa
    21781 ggcgcgcata tttatcgtat agccccatta tttattctcc agccatggaa gtagagtttc
    21841 cattaattgc gcaggggttt tgacatccag cagaatatta ataggaatgg aaatatcgaa
    21901 tctgtcttcc agcatcatta agagatccat cactttgatc gactccaggc caagatcgtt
    21961 gacgagatcg ctgtcgggct taatttccac accgttttcg accaaatcct gtaaacagga
    22021 aaggatgtaa tccattacta tttcacgatt tatcatagaa acgttaacac acctttaaac
    22081 aagacaatca aaatagcccg ttgcgtaatg cgtgttccag gctaatcccg ggaaaccaat
    22141 taatatcttc agaaatacgg ttattacttg ccgaccagtc ggcatgggtt aattcacgga
    22201 ttttcgaacg agtcagcatc ggttctttac ccgccagacg gctcaacgcg gtactgatat
    22261 ccgcaaggca ggtgagtagc ggcagaggaa taccgaccat ccgcacggaa ccacagcgga
    22321 catcggcaac aagctgttgg acacgttgcc agtcatagcc tccggcgacg ccatcgcata
    22381 attcataggt ttgcgtctgt acagtttcgg cgcttaacca ctgacccact gcttgcgcaa
    22441 aatcggtgac gtgcaggaaa gagagctgtg tctctggcgt gccaagtcgt ggcagcagac
    22501 cgcgcagcat ccagtcaaac agcggtttta actctttatc gccgggacca tacacggctg
    22561 tcgggcgaaa aacgcccaac gtaatttcgt cagccattgc agtcagctgt tgttcggcga
    22621 cgtgtttgga atttgcgtac caggagagtt cgggatggcg cgccgccagc gaagagataa
    22681 acagaaaacg ttggcaaaag ccgctctctt ttgccgcttg catcaggcgc aggctgccgt
    22741 caacgttgca gcgggtaaaa atctcttctt tgtgcccgcg cacttgcccg gcgcaatgga
    22801 ccaccgcgct ggctccggta accagttcgc taagcgaatg tgtatcttcc agcgaaccgc
    22861 gcacccaagt gagattatca ttgacgtgag cgcgggcagt acgcgtcaat gcgcgaacgt
    22921 gaaagccgca ggcgagcagg ttatcaataa tatatttacc gataaacccg gtagcgcccg
    22981 tcaccgcgac agtttgattc atgccagaga ctcctgcaca tgaagactgg cagcataagc
    23041 cttctgataa cgttttttcg cttccgcacg ggcaggcttg ccggaggatg ttcggggaat
    23101 actgtggggc ggcaacagat cgatatccgc ggtaacgcca aattcgcttt ggatccgtgc
    23161 tgccagcgcg tggataagct gcccgcgacg ttcttcatcg ctgatccgac actggatctg
    23221 caaaatgatt ttttcctggg cggtaacaaa agcaatcgca tcgccagaat gaatttccgg
    23281 ttcctgttcc gcaatatatt caatatcctg cggccagata ttacggccac gaataataat
    23341 cagatcttta atgcgtccgg tgacatacag ataaccgtcc agcagataac cgaggtcgcc
    23401 ggtgtctaac cagcccgtcg cggcaatctc gtcttgtgaa atctggtcgc caaagtaacc
    23461 gctcatcagg ctagggccgg agatgcaaat atggcctacc acacgttccg cgacaggtat
    23521 acctgcttca ttgcggattt caatgccatg ttccggcaac gctttaccgc agttgacgaa
    23581 agtcgatacg gcgcgtgttt cagcatccgg cgcgacggct ttgccctgat attcgaggat
    23641 gtcgcgatcc acttcgttaa ccacaacccc ggaagcttca tcagagaagc tgacagccag
    23701 cgcattttct gccagtccgt agcacggcat gaaagtttta tcgtcaaagt tgacctgacg
    23761 gaaacattca gcgaattgat ggagttgttc tgcggagatg ggttctgcac caataccagc
    23821 gacgcgccag caggaaagat ccagttcagc gagatctttt tcattcacgc ggcgctggca
    23881 caattcatag ccaaacggcg gcgcaacgga aacggtgccg cgatttttac tgatcaattt
    23941 aagccattgc agaggacgca tggcaaaatc ctgagtgcgc aaataatcta ctgaaagctg
    24001 cgtggcgacg ggggtcagga gaaagccgac cagtcccata tcatggtaga aaggcagcca
    24061 ggagacgcag cggtcgccag ggcgtaattt tatgccatcg tggcttatag cacgtagatt
    24121 agccatcact tcgcgatggg tgataatgac gccacgggga aaacgggtgc tgccggaggt
    24181 gtactggagg taggcgatat cgttcggaac tggacgctgg agcgcaacat cggcttccgg
    24241 taatgcctta aaccaggcgt ggcttaaaac atgtaattcg gggttgttgt tatgcgtcgc
    24301 ggcattgacc agtggcaacc actcatcacc agtgataatg gctgcgggct ggcaacttgc
    24361 cagtaaaccc tgcagcttgg cgctccagga atcccgctga ccgacgccca tgggaatcgc
    24421 caacgggacg gcgactaagc cggcatactg gcaggcaaaa aaagcctcta cgaacccgct
    24481 actggtttcg gcaatcagcg ccacgcgatc gccttttttc aggtttagcg ataacaaccg
    24541 cttcgcacca gcttcggcac gcgcttttaa cgtctgatat tccagttgat cttcaagttg
    24601 gcaacgtctg tcataaaaat tcattccagc gctactcaga gcggcatagt ccaaagcatc
    24661 aaccagcgtt ggaaaatcgg catagcgcat aggtagggaa tgcgtaaaga ttttattaga
    24721 catatacacc acgaaaaaat tggatatcaa aaataaaatg acaataatgc ggtggattat
    24781 ataggcataa aagataaatg agatagtatt ttcatcagtg tgaaaatgaa cattgattta
    24841 aataatgaat ttgagttttt tgtttttatt gtgaatgata tttaatttat gaatatatta
    24901 agaaggagcc ttgtccggtc gccttaaatc gacaaaaggc attgtggcgc ttctgtgtag
    24961 tctctgacct tatcgtaatc attaataaat actatttatt cgttgtttgt attcattggg
    25021 ttgattatta aggtgccgtt cgttgctgtt gcaacctgtt tgtcaccgtt aattatttgt
    25081 tacccacaga actatggaat gcgatctgct gatgattaaa attgaaaaag taattaataa
    25141 aaatgacctt aaggcattta tcgcttttcc gtcatcactt tatcccgacg atccaaactg
    25201 gataccccct ttattcattg aacgcaacga gcatttgtct gcgaaaaatc cggggacgga
    25261 ccatatcatc tggcaggcgt gggtggcaaa aaaagcgggg cagatagttg ggcgtattac
    25321 cgcgcaaata gatacattgc accgcgagcg ttacggcaaa gataccggtc atttcggcat
    25381 gattgacgcc attgatgatc cgcaggtttt tgccgcgttg tttggcgcag cggaagcgtg
    25441 gttgaagtca caaggtgcaa gtaagatcag cggtcctttc agcctgaata tcaatcagga
    25501 aagcggatta ctgattgaag gtttcgatac accaccctgt gcgatgatgc cacacggcaa
    25561 accgtggtat gccgcgcata ttgaacaatt gggtaatcac aaaggcattg atttactggc
    25621 gtggtggatg cagcgaaccg atctcacttt ctctccggcg ctaaaaaaac tgatggatca
    25681 ggtacgcaaa aaggtgacca ttcgttgcat caatcgtcag cggtttgctg aagagatgca
    25741 gatcctgcgt gagattttca actctggctg gcagcacaac tggggatttg tgccgtttac
    25801 cgaacatgaa ttcgcgacca tgggcgacca acttaaatat ctggtgccgg acgatatgat
    25861 ctacatcgct gagattgatt ctgcaccctg cgcgtttatt gtcggcttgc caaacatcaa
    25921 cgaggcgatt gccgatctga acggatcgct ctttcccttc ggctgggcaa aattgctgtg
    25981 gcgtttaaaa gtcagcggtg tgcgaactgc gcgagtaccg ctgatgggcg tgcgggacga
    26041 gtatcagttc agccgcatcg gcccggtgat tgcgctgtta ttgattgaag ccttacgcga
    26101 tccgtttgcc cgccggaaga tcgatgcgct ggagatgtca tggatcctcg aaaccaatac
    26161 aggcatgaat aacatgctgg aacgcattgg ggcagagccg tacaagcgtt accgattgta
    26221 tgaaaagcag atttaatacc agatcctgtt aagcgcaggg gcgatccctc gcccctcggg
    26281 gaaagggtta gggtgagggt gaaaactcgg ctcacattcc caccttgttc atctccagcg
    26341 gcaccgccga gcgaatcaaa tggtggcctt ccagctcgat atcgtggaga ttctttttca
    26401 cggtccgcac atgatcgcgt gctgcgcgct gggcgacgtg cggcagccgc tgcaacaccg
    26461 cgttgtagat ccgcgcatgc tggcgatcga tctgcttttt ttgctgtggt cgatggtaga
    26521 gattatttac gctggcaaac actgaattaa acatcagatc ggtcaatgat tgcagcgtaa
    26581 acaccagcac ctgattgtga gaagcctgac aaatggcgag atggaaagcg tgatccaact
    26641 gcgcatgttc gatcagcgaa atctctttgt tgttctcact ggcggcgagc attttttcat
    26701 aacagcgggt tatcacaaca aaatcagcct gggttcccag cgttgccgcc agccttgccg
    26761 attcaccctc cagcaatgcg cgaacgtcga gcagatcgta cagcgttcgc ggctgcgtac
    26821 tgaacagatg gatcagcggg ctggtgtcct gcacccgatt aagccgtgcg acacgagaat
    26881 cgcgaccctg cgccgtttca ataatcccgc gcccgcgcag cacggtcagc ccttcacgca
    26941 gtgcggagcg tgagaagccg agcttttcac acagtcgacg ttccgaggga agcggctgac
    27001 cgaccttcag tacgccgtcg ataattaacc gttcgatact ctctgcaacc acttcgcaaa
    27061 tagggcgacg ttcatctttc attcccggac ctcgtgcaca ggtaggacca atttttctgt
    27121 tcttaactcg caaaacacgc acatcacgca agtgattatg ttacatcaat ttaacattaa
    27181 gttaaccagg acaaggtcac agagctggaa aaaaaatggt ctgaccggta gctaaagaga
    27241 tagacgaaaa cgaaaagccc gcttaataac tgttcacaga agcagcgcga aaaaatcagc
    27301 tgccacacaa cacaacaaag cgaaccctac tcatgagcat cttgtacgaa gagcgtcttg
    27361 atggcgcttt acccgatgtc gaccgcacat cggtactgat ggcactgcgt gagcatgtcc
    27421 ctggactgga gatcctgcat accgatgagg agatcattcc ttacgagtgt gacgggttga
    27481 gcgcgtatcg cacgcgtcca ttactggttg ttctgcctaa gcagatggaa caggtgacgg
    27541 cgattctggc tgtctgccac cgcctgcgtg taccggtggt gacccgtggt gcaggcaccg
    27601 ggctttctgg tggcgcgctg ccgctggaaa aaggtgtgtt gttggtgatg gcgcgcttta
    27661 aagagatcct cgacattaac cccgttggtc gccgtgcgcg cgtgcagccg ggcgtgcgta
    27721 acctggcgat ctcccaggcc gttgcgccgc ataatctcta ttacgcgccg gatccgtcct
    27781 cacaaatcgc ctgttcgatt ggcggcaatg tggcggaaaa tgccggtggc gttcactgcc
    27841 tgaaatatgg tctaaccgta cataacctgc tgaaaattga agtgcaaacg ctggacggcg
    27901 aggcgctgac gctgggatcg gacgcgctgg attcgcctgg ttttgacctg ctggcgctgt
    27961 ttaccggatc ggaaggtatg ctcggcgtga ccaccgaagt gacggtaaaa ctgctgccga
    28021 agccgcccgt ggcgcgggtg ctgttagcca gctttgactc ggtagaaaaa gccggacttg
    28081 cggttggtga catcatcgcc aatggcatta tccccggcgg gctggagatg atggataacc
    28141 tgtcgattcg cgcggcggaa gattttattc atgccggtta tcccgtcgac gccgaagcga
    28201 ttttgttatg cgaactggac ggcgtggagt ctgacgtaca ggaagactgc gagcaggtta
    28261 acgacatctt gttgaacgcg ggtgcgactg acgtccgtct ggcacaggac gaagcagagc
    28321 gagtacgttt ctgggccggt cgcaaaaatg cgttcccggc ggtaggacgt atctccccgg
    28381 attactactg catggatggc accatcccgc gccgcgccct gcctggcgta ctggaaggca
    28441 ttgcccgttt atcgcagcaa tatgatttac gcgttgccaa cgtctttcat gccggagacg
    28501 gcaacatgca cccgttaatc cttttcgatg ccaacgaacc cggtgaattt gcccgcgcgg
    28561 aagagctggg cgggaagatc ctcgaactct gcgttgaagt tggcggcagc atcagtgggg
    28621 aacatggcat tgggcgcgaa aaaatcaatc aaatgtgcgc ccagttcaac agcgatgaaa
    28681 tcacgacctt ccatgcggtc aaggcggcgt ttgaccccga tggtttgctg aacccaggaa
    28741 aaaacattcc cacgctacac cgctgtgctg aatttggtgc catgcatgtg catcacggtc
    28801 atttaccttt tcctgaactg gagcgtttct gatgctacgc gagtgtgatt acagccaggc
    28861 gctgctggag caggtgaatc aggcgattag cgataaaacg ccgctggtga ttcagggcag
    28921 caatagcaaa gcctttttag gtcgtcctgt caccgggcaa acgctggatg tgcgttgtca
    28981 tcgcggcatt gttaattacg acccgaccga gctggtgata accgcgcgtg ccggaacgcc
    29041 gctggtggcg attgaagcgg cgctggagag cgccgggcaa atgctcccct gcgagccgcc
    29101 gcattatggt gaagaagcca cctggggcgg gatgatcgcc tgcgggctgg cggggccgcg
    29161 tcgcccgtgg agtggttcgg tccgcgattt tgtcctcggc acgcgcatca ttaccggcac
    29221 tggaaaacat ctgcgttttg gtggcgaagt gatgaaaaac gttgccggat acgatctctc
    29281 acggttaatg gccggaagct acggttgtct gggtgtgctc actgaaatct cgatgaaagt
    29341 gttaccgcga ccacgcgcca ccctgagtct gcgtcgggaa atcagcctgc aagaagccat
    29401 gaatgaaatc gcccagtggc aactccagcc attacccatt agtggcttat gttacttcga
    29461 caatgcgttg tggatccgcc ttgagggcgg cgaaggatcg gtaaaagcag cgcgtgaact
    29521 gctgggtggc gaagaggttg cggatcagtt ctggcagcaa ttgcgtgaac aacaactgcc
    29581 gttcttctcg ttaccaggta ccttatggcg catttcatta cccagcgatg cgccgatgat
    29641 ggatttaccc ggcgagcaac tgatcgactg gggcggggcg ttacgctggc tgaaatcgac
    29701 agccgatgac aatcaaatcc atcgcattgc ccgcaacgct ggcggtcatg cgacccgctt
    29761 tagtgccgga gatggtggtt ttgccccgct accggctcct ttattccgct atcaccagca
    29821 gcttaaacag cagctcgacc cttgcggcgt gtttaatccc ggtcgcatgt acgcggagct
    29881 ttaaggagca ggctatgcaa acccaattaa ccgaagagat gcggcagaac gcgcgggcgc
    29941 tggaagccga cagcatcctg cgcgcctgtg ttcactgcgg attttgtacc gcaacctgcc
    30001 caacctatca gctcctgggc gatgaactgg acgggccgcg cgggcgcatc tatctgatta
    30061 aacaggtgct ggaaggcaac gaagtcacgc ttaaaacaca ggagcatctc gatcgctgtc
    30121 tcacttgccg caattgtgaa accacctgtc cttctggtgt gcgctatcac aatttgctgg
    30181 atatcgggcg tgatattgtc gagcagaaag tgaaacgccc attgccggag cgaatgctgc
    30241 gcgaaggatt gcgcgaggta gtgccgcgtc cggcggtctt ccgtgcgctg acgcaggtag
    30301 gactggtgct gcgaccgttt ctgccggaac aggtcagagc aaaactgccc gccgaaacgg
    30361 tgaaagctaa accgcgacca ccgctgcgcc ataagcgtcg ggttttaatg ctggaaggct
    30421 gcgcccagcc tacgctttcg cccaacacca acgcggcaac tgcgcgagtg ctggatcgtc
    30481 tggggatcag cgtcatgcca gctaacgaag caggctgttg tggcgcggtg gactatcatc
    30541 ttaatgcgca agagaaaggg ctggcgcggg cgcgaaataa tattgatgcc tggtggcccg
    30601 cgattgaagc aggtgccgag gcaattttgc aaaccgccag cggttgtggc gcgttcgtta
    30661 aagagtatgg gcagatgctg aaaaacgatg cgttatatgc cgataaagcg cgtcaggtca
    30721 gtgagctggc ggtcgattta gtcgaacttc tgcgcgagga accgctggaa aaactggcaa
    30781 ttcgcggcga taaaaaactg gccttccact gcccgtgtac cctacaacat gcgcaaaagc
    30841 tgaacggcga agtggaaaaa gtgttgcttc gtcttggatt taccttaacg gacgttcccg
    30901 acagccatct gtgctgcggg tcggcgggaa catatgcgtt aacgcatccc gatctggcgc
    30961 gccagctgcg ggataacaaa atgaatgcgc tggaaagcgg caaaccggaa atgatcgtca
    31021 ccgccaacat tggttgccag acgcatctgg cgagcgccgg tcgtacctct gtgcgtcact
    31081 ggattgaaat tgtagaacaa gcccttgaaa aggaataaca aaatgaaaac taaagtcatt
    31141 cttagccagc aaatggcgag tgcaattatt gccgcaggtc aggaagaggc gcagaaaaat
    31201 aactggtctg tttccattgc tgttgccgat gacggcggtc atctgctggc gttaagtcgc
    31261 atggacgatt gcgcgccgat tgcggcttac atctcccagg agaaagcgcg taccgccgcg
    31321 ctggggcgtc gtgaaactaa gggctatgaa gagatggtga acaacggacg taccgcgttc
    31381 gtgactgcgc cgttactgac gtcgctggaa ggtggcgtac cggttgttgt ggatgggcaa
    31441 attattggtg ccgtgggcgt ttctggttta accggagcac aggatgcgca ggtcgcgaaa
    31501 gcggcagcag cggtgttggc gaaataagcg aaaacgagga gataaacaat gagtcaaacc
    31561 ataacccagg gccgtttacg cattaacgcc aattttaaac gttttgtgga tgaagaagtt
    31621 ttaccgggaa cagggttgga cactgcggcg ttctggcgca attttgatga gatcgttcac
    31681 gatctggcac cagaaaatcg tcagttgctg gcagaacgcg atcgcattca ggcggcgctt
    31741 gatgagtggc atcgcagcaa tccggggccg gttaaagata aagaggccta taaatctttc
    31801 ctgcgtgaac tgggctacct ggtgccacaa ccggagcgcg tgacggtgga aaccacgggc
    31861 attgacagcg aaatcaccag ccaggcgggg ccacagctgg tggttccggc aatgaacgcc
    31921 cgctacgcgc tgaacgcggc gaacgctcgc tggggctcac tgtacgatgc gttatacggc
    31981 agcgacatca tcccgcagga aggggcgatg gtcagcggct acgatccgca gcgcggtgag
    32041 caggttatcg cctgggttcg gcgtttcctc gatgaatctc taccgctgga aaacggcagc
    32101 tatcaggatg tgctggcgtt taaggtggtc gataaacaat tacgcatcca gttgaaaaat
    32161 ggtaaagaaa ccacgttacg tacccctgcg cagtttgtcg gttaccgtgg cgatgccgct
    32221 gcgccgacct gcattttgct gaaaaataac ggcctgcata ttgaactgca aattgatgcc
    32281 aacgggcgga ttggcaaaga cgatccggcg cacatcaacg atgttatcgt cgaagcggcc
    32341 atcagtacca ttctcgactg cgaagattcg gtcgcggcgg ttgatgcgga agataaaatc
    32401 ctgctgtacc gcaacctgct gggcctgatg caggggactc tgcaagagaa aatggagaaa
    32461 aacggtcgac aaatcgtgcg taaactgaat gacgatcgtc attacaccgc cgccgatggc
    32521 tctgaaattt ctctgcacgg acgctcgctg ctgtttatcc gcaacgtggg tcatttgatg
    32581 accattcctg tgatttggga cagcgaaggc aatgaaatcc cggaaggcat tcttgatggc
    32641 gtcatgactg gcgcgattgc cctctatgat ttaaaagtgc agaaaaactc gcgcactggc
    32701 agcgtctata ttgtgaaacc gaaaatgcac ggtccgcagg aagtggcgtt cgccaacaaa
    32761 ctgtttaccc gcattgagac aatgctcggt atggcaccga ataccctgaa aatgggcatt
    32821 atggatgaag aacgtcggac ctcgctgaac ttgcgtagct gtatcgctca ggcgcgcaac
    32881 cgcgtggcgt tcatcaatac cggtttcctc gaccgtaccg gcgatgaaat gcattcggtg
    32941 atggaagctg gcccgatgct gcgtaaaaat cagatgaaat cgacgccttg gatcaaagcc
    33001 tacgagcgta ataacgtgct ttccggtctg ttctgtgggc tgcgcggtaa agcgcaaatc
    33061 ggtaaaggca tgtgggcaat gccggacctg atggcagaca tgtacagcca gaagggcgac
    33121 caactgcgtg ccggggcaaa cacagcctgg gttccgtcac caaccgctgc tacgctccat
    33181 gcgctgcact accaccaaac caacgtacag agcgtgcagg ccaacattgc ccagagcgag
    33241 ttcaatgctg aatttgaacc gctgctggac gatctgctga ctattccggt ggctgaaaac
    33301 gctaactggt cggtggaaga gatccaacaa gagctggata acaacgtgca ggggattctg
    33361 gggtacgtgg tgcgttgggt ggagcagggg attggttgtt caaaagtgcc ggatattcac
    33421 aacgtggcgc tgatggaaga ccgcgcaacg ctgcgtatct ccagccagca tatcgccaac
    33481 tggttacgtc acggtattct gaccaaagaa caggtgcagg cgtcgctgga gaatatggcg
    33541 aaagtggttg atcagcaaaa cgctggcgat ccggcttatc gtccgatggc ggggaatttc
    33601 gctaactcgt gtgcttttaa agctgccagc gatttaatct tcctcggcgt gaaacagcca
    33661 aacggctata ccgaaccgtt attacacgcc tggcgtttac gcgaaaaaga aagtcattaa
    33721 tagcgtcaaa aaagccccgg caagttttat tgtcggggct atgatagtaa ctgcggtagt
    33781 acatttatcc cggaaataac cggagcgatt atcagggttt aacaatatta aatacccgta
    33841 gcgcattact agcgggccta aaacaacaat atggacatct taacgatatt ctgcgcctgc
    33901 gtgtgcggaa cagaaaccct tacccgatat acttgttatg ccagaggtag caattgcgtt
    33961 agcagcgtac tgttgctgta aagtataacg cctatatttt acagttcagt cctcttttgc
    34021 ttattacaac cttaagtata tcaagcatat aaagataata agagactgaa caatatggtt
    34081 acctggaccc aaatgtatat gccgatggga ggactggggc tatccgctct ggtcgccctg
    34141 atcccgataa tattcttctt cgttgcactc gcggtattac gtctgaaagg acatgtcgcc
    34201 ggagcaataa cccttatatt atctattctg attgcgatat tcgcctttaa aatgccgatt
    34261 gatatggcat ttgctgctgc gggctatggc tttatttatg gattatggcc tattgcgtgg
    34321 attattgttg cggcggtgtt cctctataaa ttaaccgttg ccagcggtca gttcgatatt
    34381 atccgcagct cggttatctc catcactgac gatcagcgtt tgcaggtgtt actgattggt
    34441 ttctcctttg gcgcgctgct ggaaggggcg gctggctttg gtgcgccggt ggcgattacc
    34501 ggtgcgctgc tggtgggcct gggcttcaaa ccgttatacg cggcggggct gtgtctgatt
    34561 gccaacactg cgccggtggc gtttggtgcg ttgggcgtgc cgattctggt cgccggtcag
    34621 gtaacgggaa tcgatccgtt ccacattggc gcaatggcgg gacgtcagtt accgttcctg
    34681 tcggttcttg tgccgttctg gctggtagca atgatggacg gctggaaagg ggtgaaagag
    34741 acgtggccag cggcgctggt tgctggggga agcttcgctg tcacgcagtt ctttacctct
    34801 aactatattg gtccggaact gccggatatt acttcggcgc tggtgagtat cgtctcactc
    34861 gctttattcc ttaaagtctg gcggccgaaa aataccgaaa cggcaatcag catgggacaa
    34921 tccgcaggtg cgatggtggt gaataaacca tcttctggcg gtcccgtgcc ttcagaatat
    34981 agtctggggc aaatcattcg tgcgtggtca ccgtttttaa tcttaacggt gctggtcacc
    35041 atctggacca tgaagccgtt taaagcgtta tttgctccgg gcggcgcgtt ttattcactg
    35101 gtgattaatt tccagatccc tcatttgcat caacaagtgt tgaaagcggc acccattgtc
    35161 gcccaaccaa cgccaatgga tgcggtgttt aaattcgacc ctctctcggc tggcggcacc
    35221 gctattttta ttgcggcgat tatctctatc ttcatcctcg gcgtgggcat caagaaaggg
    35281 attggcgtct ttgccgaaac gctaattagt ctgaagtggc cgatattgtc gattggcatg
    35341 gtgctggcgt tcgccttcgt caccaactat tccggcatgt ccaccacgct ggcgctggta
    35401 ctggcaggta caggcgtgat gttcccgttt ttctcaccgt ttctcggctg gctgggcgta
    35461 ttccttaccg gctcggacac ctcctctaac gccctgtttg gttcactgca atcgaccacg
    35521 gcgcagcaaa tcaacgtctc tgacactctg ctggtggcag caaataccag cggcggcgta
    35581 actggcaaga tgatttcccc gcaatctatc gccgtggcct gcgccgcgac gggcatggtg
    35641 ggccgagaat ctgaactgtt ccgctacacc gtgaagcaca gtctgatctt tgccagtgtg
    35701 atcggcatta tcacgctgtt gcaggcgtat gtgtttaccg ggatgttagt ttcgtaagtc
    35761 aataaagccg gatgatcgtt aatatcatcc ggcttcgttt acattactct gtcttattaa
    35821 tggaaaatta tttcccgctt tattatccat gtggcaataa tgtttcgcgt tcagataacc
    35881 tgctaaataa ttaaaagcat gagaacatct gtttttttat cacgcaaaac aagtcagtat
    35941 taattatgtt cgcttgatga gcaaacaccg ttctatctgt tgcttttgca atcttaatcg
    36001 ttaaaactac caacaaattt atatagttac gttgtgaaaa gttgttgatt ttttgtttat
    36061 tgaaatttcg taaaaataac cagccagaca tcgaataggg gttattctta taaatttcac
    36121 ctgtttttct atcaatgatg ccgttttctt aagaatggag ggagttatta aagaagataa
    36181 agcataaaac tatttccccg gttacgaatc ttttaacttt gttttgtcac ttgcgttatt
    36241 aatgaataag aaatttaaat ataagaaatc gcttttagcg gctattttga gtgcaaccct
    36301 gttagccggt tgtgatggca gtggttccgg atcttcctcc gatacgccgc ctgtagattc
    36361 tggaacaggg tctttgccgg aagtgaaacc tgatccaaca ccaaacccgg agccgacgcc
    36421 tgagccaacg ccggacccag aacctacgcc ggaaccgaca cctgatccag agccagaacc
    36481 ggagccagaa cctgttccta cgaaaacggg ttatctgacc ctgggcggaa gccagcggat
    36541 aactggtgct acttgtaatg gtgaatccag cgatggcttt acatttacac ctggcgagga
    36601 cgttacttgc gtggcgggta acacgacaat tgctaccttc gacacccagt cagaagctgc
    36661 gcgtagcctg cgtgcggttg aaaaagtgtc gtttagtctt gaggacgcgc aagaactggc
    36721 ggcttccgat gacaagaaaa gcaatgcggt ttcgctggta acgtccagta acagctgtcc
    36781 ggcgaataca gaacaggttt gcctgacgtt ctcctcggtg atcgagagta aacgtttcga
    36841 ctcgctgtat aagcaaatcg atctggcacc ggaagagttc aaaaagctgg tcaatgaaga
    36901 ggtggaaaac aatgctgcga ccgataaagc gccatccact catacctcac cggtcgtgcc
    36961 tgtcaccacg ccgggaacaa aaccggatct gaacgcgtcc ttcgtgtcgg ctaacgcgga
    37021 acagttttat caatatcaac ccactgaaat cattctttcc gaaggccgac tggtggatag
    37081 catgggcaat ggtgtggttg gcgtaaatta ctacaccagc tcaggccgtg gcgtaactgg
    37141 cgaaaacggc aaattcaact tcagctgggg cgaaaccatc tcctttggta tcgacacctt
    37201 tgaactgggc tcagtgcgcg gcaataagtc gaccattgcg ttgactgaac tgggtgacga
    37261 agttcgcggc gcgaatattg atcagcttat tcatcgttac tcccaggccg gaaaaaatga
    37321 tgagcgtgaa gtgccggacg tagtgcgcaa ggtctttgcc gagtatccca acgtaatcaa
    37381 cgagattatc aatctctcgt tatccaatgg cgaggcgttg agcgaaggcg atcaaacctt
    37441 tgagcggaca aacgaatttc ttgagcagtt tgaatccggg caggctaaag aaattgatac
    37501 ggcgatttgt gactcccttg ggggctgcaa ctctcagcgt tggttctcgt tgacagcacg
    37561 caatgttaat gacggccaga ttcagggcgt tattaacaag ctgtggggtg tggatacgaa
    37621 ctacaaatct gtcagcaagt tccatgtatt ccatgactct accaacttct atggcagcac
    37681 cggtaatgcg cgcggtcagg cagtggtgaa tatctccaac gcggcattcc cgattctgat
    37741 ggcgcgtaat gataaaaact actggctggc cttcggcgaa aaacgcgcct gggataaaaa
    37801 cgagctggcg tacattacgg aagcgccttc tctcgttgag ccggaaaacg ttacgcgcga
    37861 taccgccacc tttaacctgc cgtttatttc gctggggcaa gtcggtgagg gcaaactgat
    37921 ggttatcggt aacccgcact acaacagcat tttgcgttgt ccgaacggtt acagctggga
    37981 aggcggtgtt gataaaaacg gtcagtgtac gcgtaacagt gattctaatg atatgaagca
    38041 ctttatgcag aacgtgttgc gctatctgtc cgacgataaa tggacgccgg acgcgaaagc
    38101 cagcatgacc gtaggtacca acctggatac tgtctatttc aaacgtcatg gtcaggttac
    38161 aggaaacagc gctgagttcg gctttcatcc ggattttgcg ggtatctctg ttgagcattt
    38221 aagtagctat ggcgatctcg acccgcagga aatgccgctg ctgatcctca acggctttga
    38281 gtatgtgact caggttggta acgatcctta tgcaatcccg ctgcgagcag ataccagcaa
    38341 accgaagctg actcagcagg atgtgaccga tctgatcgcc tatctgaaca aaggtggatc
    38401 ggtgctgatc atggaaaacg tgatgagcaa tcttaaggaa gagagcgcgt ctggctttgt
    38461 acgtctgctt gatgccgcag gtctgtcgat ggcactgaac aagtcggtag taaataacga
    38521 tccgcaaggg tatccgaacc gcgttcgtca gcagcgcgca acgggcattt gggtctatga
    38581 acgttatcct gccgtagatg gtgcgctgcc gtacaccatc gatagtaaga caggggaagt
    38641 taagtggaaa tatcaggtag aaaacaaacc tgatgacaaa ccgaagctgg aagttgccag
    38701 ctggctggaa gatgtagatg gcaaacagga aacgcgttat gcctttattg atgaggccga
    38761 tcataaaaca gaggattctc tgaaggctgc gaaggcaaaa atctttgaga agtttcctgg
    38821 attaaaggag tgtaaggacc caacttacca ctacgaggtc aactgtctgg aatatcgtcc
    38881 tggcacgggg gttccggtta ctggtggcat gtatgttcca cagtatacgc aactaagcct
    38941 taacgccgac acggcaaaag cgatggtgca ggctgcggat ttaggcacca acattcagcg
    39001 tctgtatcag catgagctct acttccggac caatggtcgc aaaggtgagc gtctgagcag
    39061 cgtcgagctg gaacgtctgt accagaacat gtcggtctgg ctgtggaata aaattgaata
    39121 tcgttatgaa aacgacaagg atgacgagct gggctttaaa acgttcaccg agttcctgaa
    39181 ctgctacgcc aacgatgcct atactggcgg cacgcagtgt tctgatgagc tgaaaaaatc
    39241 gctggtcgat aacaacatga tctacggcga gaagagcgtt aataaagcgg gcatgatgaa
    39301 cccgagctat ccgctcaact atatggaaaa accgctgacg cgcctgatgc tgggtcgttc
    39361 ctggtgggat ctgaacatca aagttgatgt cgagaagtat ccgggagcgg tatcggcaga
    39421 aggtgagaag gttactgaaa ccatcagcct gtactcgaat ccgaccaaat ggtttgcggg
    39481 taacatgcag tctactggcc tgtgggctcc ggctcagaaa gaggtcacta ttgagtctaa
    39541 ggcgaacgtt cctgtgactg tcaccgtggc gctggccgac gacctgaccg gacgtgagaa
    39601 gcatgaagtt gctctgaacc gtccgccaaa agtgacgaaa acctatgagc tgaaagccaa
    39661 tggtgaggtg aagtttacgg ttccttacgg tggtctgatt tatatcaagg gcaacagccc
    39721 aaagaatgag tcagccgaat tcacctttac tggtgtggtg aaagcgccgt tctataaaga
    39781 tggcgcatgg aaaaacgctc tgaactcccc tgcgccgctg ggcgagctgg aatcagacgc
    39841 tttcgtctat accacgccga agaagaacct tgaggccagc aattacaagg gcggtcagga
    39901 acaattcgct gaggaactgg atacctttgc cagctcgatg aatgacttct acggacgtga
    39961 tggcgaaaac ggtaagcacc ggatgtttac ctataaaaac ttgacggggc acaagcatcg
    40021 tttcaccaac gatgtgcaga tctccatcgg tgatgcgcac tctggttatc cggtaatgaa
    40081 cagcagcttc tcgacgaaca gcaccacgct gccgacgacg ccgctgaacg actggctgat
    40141 ctggcacgaa gttggtcaca acgccgccga aacgccgctg actgttcctg gcgcaactga
    40201 agtagcgaac aacgtgctgg cgctgtacat gcaggatcgc tatctcggca agatgaaccg
    40261 tgtcgctgac gacattaccg tcgcgccgga atatctggag gagagcaacg gtcaggcatg
    40321 ggcgcgcggc ggtgcgggtg accgtctgct gatgtacgca cagctgaaag agtgggcaga
    40381 gaaaaacttt gatatcaaga cgtggtatcc agatggcaat ctgccagcgt tctacagcga
    40441 gcgtgaaggg atgaaaggct ggaacctgtt ccagttgatg catcgtaaag cacgcggcga
    40501 tgaggttggc aaaaccaagt ttggcgaaag aaattactgt gccgaatcca acggtaacgc
    40561 tgccgacaag ctgatgctgt gtgcctcctg ggtcgctcag acggatcttt cggagttctt
    40621 taagaaatgg aatccgggcg cgaatgctta ccagttaccg ggagcgagcg agatgaactt
    40681 cgagggcggt gtgagccagt cggcttacga gacgctggcg gcgcttaatc tgccgaaacc
    40741 gcagcaaggg ccggaaacca ttaatcaggt taccgagcat aagatgtcag ctgagtaagc
    40801 ctgtatgccg gataaggcgc tcgcgccgca tccggcatga aataaggcgc atcgggcctg
    40861 gaggaaagcc atatggcata cacccgcagg cccgcatccg gcaagttaca acaaacaacc
    40921 tttaaccatg ctttttgatg tttttcagca ataccccgcg gggatgccca tactggcaac
    40981 cgtcggagga ttgatcatcg gcagtttttt gaatgtggtg atttggcgtt accccatcat
    41041 gctgcgccaa caaatggcgg agtttcacgg tgaaatgccg agtacgcagt caaaaataaa
    41101 cctggcgctg ccacgctcgc actgtccgca ttgccagcag accatccgga tacgtgacaa
    41161 tattccgctg ctctcatggt tgatgctcaa agggcgctgc cgcgactgtc aggcgaaaat
    41221 cagcaagcgt tatccgctgg tggagttatt gacggcactc gcttttttgc tggcgagtct
    41281 ggtgtggcca gaaagtggat gggggctggc ggtgatgata ttatccgcct ggctgattgc
    41341 cgcgagcgtc attgacctcg ataaccaatg gctgcccgat gtttttactc agggcgtatt
    41401 gtggacggga ctaattgcgg catgggcgca gcagagcccg ctcacgctac aagacgcagt
    41461 taccggcgta ctggtggggt ttatcacttt ttactcactg cgctggatag ccggaatagt
    41521 tctgcgtaaa gaagcattag gcatgggcga tgtattactt ttcgctgcgt taggtggctg
    41581 ggtggggccg ttgtcgctac ccaatgttgc tttaatcgcc tcatgctgcg gcctgatata
    41641 tgccgttatt acaaaaagag gatcaaccac actgcctttt ggaccgtgtt taagtctggg
    41701 cggtatagca acactttatc tacaggcatt gttttaatga taacaacgtc attatcaaag
    41761 tgacatttta actcttatta ataaccttag agattattta ccatgtcgat aaaacaaatg
    41821 ccagggaggg tattaatatc gctattgttg agcgttacag gattattaag tggctgtgcc
    41881 ggccataatg aaaatgccag tttactggcg aaaaaacagg cgcaaaatat cagccaaaac
    41941 ctgccgatta aatctgcggg atatacctta gtgctggcac aaagtagcgg cacgacggta
    42001 aaaatgacca ttatcagcga agcgggtact cagaccacgc agacacctga cgccttttta
    42061 accagctatc aacgacaaat gtgcgctgac ccgacggtga aattaatgat cactgaggga
    42121 attaattaca gcataacgat taatgataca cgtacaggta accagtatca gcggaaactg
    42181 gatcgtacca cctgtggtat agtcaaagca taacgtcggg tagatataaa ttggcgcagg
    42241 ttgtttttcg tgacgcacga atttatctca ttcaatggct gacaaaaatt cgtcacactc
    42301 ttaaccagag acaatctctt aatacagaca aagagcatct gcgaaaaatt gcacgcggga
    42361 tgttctggct gatgctgctt attatttctg caaaagtggc gcattcactc tggcgctatt
    42421 tctccttttc tgcggaatat acggcggttt ccccatcggc gaataaaccg ccccgtgcag
    42481 atgcaaaaac gttcgataaa aatgacgtgc aattaatcag ccagcaaaac tggtttggca
    42541 aatatcagcc cgtcgccgca ccggtaaaac aacctgaacc tgtgcctgtg gcagaaacgc
    42601 gtcttaatgt ggtgctgcgc gggatcgcct ttggtgccag acccggcgcg gtgattgaag
    42661 aaggcggcaa acagcaggtc tatttacagg gcgaacggct tggctctcac aacgcggtga
    42721 ttgaggaaat taatcgtgac catgtgatgc tgcgctatca ggggaaaatg gaacgtctga
    42781 gtctggcaga agagaagcgt cccaccatag ccgtgaccag caaaaaagcc gtcagcgacg
    42841 aagcaaagca agctgttgct gagcctgctg ccagtgcgcc agttgagatc ccggctgccg
    42901 tgcgtcaggc actggcgaaa gatccgcaga aaatttttaa ctatatccag cttacgcctg
    42961 tgcgtaagga agggattgtc ggttatgcag tgaaaccggg ggcagatcgt tctctgttcg
    43021 atgccagcgg ttttaaggaa ggcgatatcg ccattgcgct aaatcagcag gatttcactg
    43081 atccacgagc aatgattgct ctgatgcggc agttaccttc aatggattcc attcaactta
    43141 cggttttacg caagggtgcg cgctacgaca tttccatcgc gctgcgctaa ccgcatttaa
    43201 tccaggagaa tcattcatcg tgttttggcg tgatatgacg ttgtcggtct ggcgtaagaa
    43261 gacaactggc ctcaaaacaa aaaagcgttt actgccgctg gtgctggcag cggcattatg
    43321 cagttcacct gtctgggcgg aagaagccac tttcaccgct aattttaaag ataccgacct
    43381 gaaatcgttc atcgaaaccg tcggcgctaa ccttaataaa accatcatta tggggccggg
    43441 cgttcagggg aaagtgagta ttcgcaccat gaccccgctc aatgaacgcc agtattacca
    43501 gctattcctt aacctgctgg aagcacaggg gtatgccgtc gtaccgatgg aaaacgacgt
    43561 gctgaaggtg gtgaaatcca gtgccgcgaa agtcgagccg ctgccgctgg ttggtgaagg
    43621 cagcgacaac tacgcgggcg atgaaatggt caccaaagtc gtgccggtac gtaatgtgtc
    43681 ggtacgcgag ctggcaccga ttctgcgcca gatgatcgac agcgcaggct caggcaacgt
    43741 tgttaattac gatccctcca acgtgattat gcttaccgga cgcgcctcag ttgtggagcg
    43801 cctgacggaa gtgatccagc gcgtggatca tgcgggtaat cgcaccgaag aggtgatccc
    43861 gctggataac gcctcggctt cggaaatcgc ccgcgtgctg gaaagcctga cgaaaaacag
    43921 cggcgagaac cagcctgcga cgctgaaatc tcaaattgtc gccgacgaac gcaccaacag
    43981 tgtgattgtc agtggtgacc cggccacgcg ggacaaaatg cgccgcctga tccgtcggct
    44041 ggactcagaa atggagcgca gcggcaacag ccaggttttc tatctcaaat acagcaaagc
    44101 cgaagatctg gtcgatgtac tgaagcaggt cagcggcacg ctcacggcgg ctaaagaaga
    44161 ggcggaaggc accgtcggta gcgggcgtga ggttgtctcc atcgccgcca gcaaacacag
    44221 taatgccctg attgttaccg cgccgcagga catcatgcag tcgctgcaaa gcgtgattga
    44281 acaactggat attcgccgtg ctcaggtgca cgtcgaggcg ttgatcgtgg aagttgccga
    44341 aggtagcaat atcaatttcg gcgtgcagtg ggcgtcgaaa gatgccggat tgatgcagtt
    44401 tgctaacggt acgcagatcc ctattggcac gctgggtgca gccatttctc aggcgaaacc
    44461 gcagaaaggc tccacggtga tcagtgaaaa cggcgctacc accattaacc cggatactaa
    44521 cggcgatctc tccacgctcg ctcagcttct ttctggcttt agcggtacgg cggttggcgt
    44581 ggtgaaaggc gactggatgg cgctggtaca ggcggtcaaa aacgactcca gctcgaacgt
    44641 gctctccacg ccgagcatca ccacgctgga caaccaggaa gccttcttca tggtgggcca
    44701 ggacgttccg gtattaactg gatctaccgt tggctccaat aacagcaatc ctttcaacac
    44761 ggtagagagg aaaaaagtcg gcatcatgct gaaagtcacg ccgcagatta acgaaggtaa
    44821 cgcggtacag atggtgattg agcaggaagt ctcgaaggtg gaaggacaga ccagcctcga
    44881 cgtcgtgttt ggcgagcgca aactgaaaac caccgtgctg gctaacgatg gtgagctgat
    44941 cgtgcttggc ggtctgatgg acgaccaggc gggagaaagc gtggcgaaag tgccgctgct
    45001 gggcgatatc ccgttgattg gtaacctgtt taaatcgacg gcggataaaa aagaaaaacg
    45061 taacctgatg gtgtttatcc gcccgaccat tctgcgtgac ggtatggcgg cagacggcgt
    45121 gtcgcagcgc aaatataact acatgcgcgc cgagcaaatc taccgcgatg agcaaggctt
    45181 aagcctgatg ccgcacaccg cgcagccgat attgcctgcg caaaatcagg ccttaccgcc
    45241 agaagttcgt gcgttcctca atgccgggag aacgcgttaa tggtgcctgt agcacaggaa
    45301 accaccgcta acaccgtgcg tctgccctac agtttcagcc gtcggtttag cctggtggca
    45361 tggtgcgaag cgtcgctgga gatcctccac gttcatccgt tgtcgctctc tgttttgcag
    45421 gagctgcagc gggggctgaa cgcgcccttt acgctgcggc aaatcgacgg ggccgaattt
    45481 gaacagcgac tgaatgcggt ctggcagcga gactcttccg aggcccgcca gttgatggaa
    45541 gatctcggtt ctgctgagga tttttttacc ctcgccgaag aactgccgga aacggaagat
    45601 ctgctggaaa gcgacgacga cgcgccaatc atcaaactga tcaacgctat gctggcagag
    45661 gcgattaaag aaggtgcttc ggatatccac atcgagacat ttgaaaagag tctggtgatc
    45721 cgttttcgcg ttgacggcac attacatgaa atgctgcgcc cggggcgcaa actggcctcg
    45781 ctgctggtat cgcgtatcaa ggtgatggcg cggctggata ttgccgaaaa gcgcgtaccg
    45841 caggatgggc gtattgcgct gctgctgggc ggtcgggcga ttgacgtgcg tgtctccacc
    45901 atgccctccg cctggggcga gcgcgtggtg ctgcgactgc tggacaaaaa ccaggcccgc
    45961 ctgacgctgg aacgtctggg gttgagtcac gaactgaccg cgcagttgcg ccagctgtta
    46021 cacaaaccgc acggcatttt tctggtgacg gggccgaccg gttccggcaa aagcaccacg
    46081 ctgtacgctg gattgcagga gctgaacaac cactcgcgta acattctcac ggttgaagac
    46141 cctatcgaat acatgattga agggatcggt cagacacagg ttaacacccg cgtcggcatg
    46201 accttcgccc gtggcctgcg cgcgattttg cgtcaggacc cggatgtggt gatggtcggt
    46261 gaaatccgcg ataccgaaac cgcagaaatt gccgtacagg catcgcttac agggcacctg
    46321 gtcctttcca cgctgcatac caacacggcg gtgggggcga tcacgcgttt gcaggatatg
    46381 ggcgtggagc ctttcctgct ctcttccagt ctgactggcg tgatggcgca gcggctggtt
    46441 cgcacgctgt gtcccgactg ccgccaggcc ctgcctgcca ctgacgaaga aaaacgcctg
    46501 ctggggatta ccgatgcgcg aaccgtcacg ctgtaccatc cgcagggctg tcccgcctgt
    46561 aatcacaaag gttttcgcgg acggactgcc atccatgagc tgatcgtggt ggatgccaca
    46621 ttgcgtgatt tgatccaccg tcaggccggg gagctggagc tggaacgtta tgtccgacaa
    46681 cactctgcgg gtatccgcag caacggcatt gagaaagtgc tcgccggaga aacctctctc
    46741 gatgaagttc tgcgggtaac catggaggcg taatggcact gttttactat caggcgctgg
    46801 agcgtaatgg tcgcaaaacc aaaggtatga ttgaggcgga ttccgcgcgt catgcccgcc
    46861 agttgttgcg cggtaaagag cttatccccg tgcatattga agcccggatg aatacttcgt
    46921 caggggggat gttgcagcgt cggcggcacg cacatcgtcg cgtggcggcg gcagatcttg
    46981 cgctgttcac gcgccaactg gcaacgctgg tacaggcagc aatgccgctg gaaacctgct
    47041 tacaggcggt cagtgagcaa agtgaaaaac tgcatgtaaa aagcctcgga atggcgctgc
    47101 gcagccggat tcaggaaggt tacaccctgt cggacagcct gcgcgaacat ccccgcgtct
    47161 ttgattctct gttttgttcg atggttgctg ccggagaaaa atccgggcat ctcgacgtgg
    47221 tgcttaatcg cctggcagat tacaccgaac agcgacagcg cctgaaatca cgtctattgc
    47281 aggccatgct ctacccgctg gtactgctgg tggtggcaac gggcgtggtc actattttgc
    47341 tgacggcagt agtgccgaaa attatcgaac agtttgatca tctcggacac gcgctgcccg
    47401 cctccacccg aatgctcatc gctatgagcg acgcgttaca ggccagcggc gtgtactggc
    47461 tggcgggttt gctggggctt ctggtgctgg ggcaacggct actcaaaaat cctgccatgc
    47521 gcctgcgctg ggataaaacc ttgctgcgtt tgcccgtgac ggggcgtgtt gcgcgcggac
    47581 tgaatacggc gcgtttttcc cgcacgttaa gcatcctcac cgccagcagt gttccgctgc
    47641 tggaaggcat tcagaccgcc gccgccgtgt cggcaaatcg ttatgtcgag caacaactgc
    47701 tgctggcggc agatcgcgtc cgcgaaggaa gcagcttgcg cgccgcgctg gcggagttgc
    47761 gcctgttccc gccgatgatg ctgtacatga tcgcctccgg cgaacagagc ggtgaactgg
    47821 aaaccatgct tgaacaggcc gcagtcaacc aggaacggga atttgatacc caggtggggc
    47881 tggcgttagg gctgtttgag ccggcgctgg tggtgatgat ggcgggcgtg gtgctgttta
    47941 tcgtcatcgc catcctcgaa ccgatgctgc aactgaacaa tatggttgga atgtaattta
    48001 cggagttatc acatgaattc gttatcccgc acacaaaaac cacgggcagg ttttaccttg
    48061 ctggaagtga tggtggtgat tgttattctt ggcgtcctgg caagtctggt ggtgcctaac
    48121 ctgctgggca acaaagagaa agccgatcgg caaaaagcca tcagcgatat cgtggcgctg
    48181 gagaatgcgc tggatatgta ccgactggat aacgggcgtt atccgactac cgaacaggga
    48241 ctggaggcgc tgatccagca accggccaat atggcggatt cccgtaacta ccgtaccggt
    48301 gggtacatta aacgactgcc aaaagatccg tggggcaatg attatcagta tctcagtccg
    48361 ggtgaaaaag gactgtttga tgtttacacc ctgggggcgg atggtcagga aaatggggaa
    48421 ggcgctggcg cagatatcgg taactggaat ttgcaggagt ttcagtaagc agtgcctgaa
    48481 cgcggattca cacttctgga aatcatgctg gtgattttcc ttatcggcct tgccagtgcg
    48541 ggcgtggtac agacatttgc gaccgattct gaatcgcccg cgaaaaaagc ggcacaggat
    48601 tttctgactc gctttgcgca gtttaaggac agggccgtaa tcgaagggca aacactcgga
    48661 gtgctaatcg acccgcctgg ctatcagttt atgcagcgtc gttacggaca gtggttgccc
    48721 gtttcctcga cccgcttatc ggcacaggtt acggtgccga aacaggtgca gatgctgtta
    48781 caacccggaa gtgatatctg gcagaaggaa tatgcgctgg agctgcagcg tcgccgcctg
    48841 acgctgcacg atattgaact ggagctgcaa aaagaggcga aaaagaagac accgcagatc
    48901 cgtttttcgc cttttgaacc cgccacgccg tttacgctgc gcttctactc ggcggcgcaa
    48961 aacgcatgtt gggcggtaaa gctggcgcac aatggcgtgt tatccctcaa tcaatgtgat
    49021 gagaggatgc catgaagcgc ggatttacct tgctggaagt gatgctcgcg ctggcgattt
    49081 ttgcgctggc tgccacggcg gtgttacaga ttgccagcgg tgcgctgagt aatcagcaaa
    49141 tccttgagga gaaaacggta gcgggctggg tagctgaaaa ccagaccgcg ctgctttacc
    49201 tgatgacccg tgaacaacga gcggtcaggc accagggcga gagtgatatg gcaggaagcc
    49261 gctggtactg gcggaccaca ccgctgaata ccggtaacgt gctgctccag gcggtggata
    49321 ttgaagtcag tcttcatgac gacttttcgc cagtgattca gtcacgacgc gcctggttta
    49381 gcgccgtggg aggccaacag tgagaagggc tcgcgctggt ttcacgttac tggaaatgct
    49441 ggtggcgata gccatttttg cctcactggc actgatggcg cagcaggtga caaacggcgt
    49501 tacacgcgtg aatagcgccg tcgccggaca cgatcaaaaa ctaaacctca tgcagcaaac
    49561 gatgagtttt ttgaaccacg atctgacaca aatgatgccg cgtccggtaa gaggcgatca
    49621 gggccagcgt gaaccagcgt tactggcggg cgctggcgtg ctggcgtctg agagtgaagg
    49681 gatgcgtttt gtacgtggcg gcgtggttaa tccgttgatg cgtctgccgc gcagtaatct
    49741 gctcaccgtc ggttaccgca ttcatggtgg ttatctcgaa cggttagcct ggccactgac
    49801 cgatgcggca ggcagcgtga agccaacaac gcaaaaatta atcccggcgg attcgctccg
    49861 tttgcagttc cacgacggca cgcgctggca ggagagctgg tcatcagtgc aggcgattcc
    49921 tgtggcagta cgcataaccc tgcattcgcc gcaatggggc gaaattgaac gcatctggtt
    49981 gttacgcggg ccgcaattat catgatcacc ttaccaccaa aacgcggaat ggcactggtc
    50041 gtggtgctgg tattgctggc ggttatgatg ctggtgacca tcacgctttc cgggcggatg
    50101 cagcaacaac ttgggcgaac gcgcagccag caggagtacc agcaggcgct gtggtacagc
    50161 gccagtgcag aaagcctggc gttgagcgca ctcagtctga gcctgaaaaa tgaaaagcgc
    50221 gtgcatctgg cacaaccgtg ggcttctggc ccgcgttttt tcccactgcc gcaggggcaa
    50281 attgccgtca ctctgcgtga cgcacaggcc tgctttaacc tgaatgccct cgctcagccg
    50341 acgacggcgt cgcgtccgct cgcggtacaa caactgattg ccctgatctc gcgcctcgat
    50401 gtgcctgctt atcgggccga actgatagtc gaaagcctgt gggagtttat tgacgaggac
    50461 cgcagcgtgc agacacgtct gggccgtgaa gacagcgagt atcttgcccg ttcggtgccg
    50521 ttctacgccg ctaatcaacc gctgacggat atcagcgaaa ttcgcgtggt gcagggaatg
    50581 gacgccgggc tttatcaaaa actgaaaccg ctggtctgtg cgctgccgat ggcccgccag
    50641 caaatcaaca tcaatacatt agatgtcacg caaagtgtga ttcttgaggc gctgtttgac
    50701 ccgtggttaa gccctgttca ggcgcgggca ttattacaac aacgtccggc gaagggctgg
    50761 gaagatgtcg atcagtttct tgctcagccg ctacttgcag acgtcaatga gcgtactaaa
    50821 aaacagctaa aaaccgtcct gagcgtggac agcaattact tctggctgcg ttcagatatc
    50881 accgtgaatg agattgaact gacgatgaat tcgttaattg tccgcatggg cccacaacac
    50941 ttttctgttc tctggcatca gacaggagaa agtgagtgag ttccatgctt gagatttatt
    51001 tcccgctttg cgccgctgat cccatccgtt ggcagcgccg tacacccgac gtggagcacg
    51061 gcatctggcc tgacgtcgcc gacgaatatc tccagcaatg gctgcaaaca gacacaattc
    51121 gactctatat tcccggcgaa tggatcagcg tctggcaggt tgaactgcct gatgtgcctc
    51181 gcaagcagat accgacgatt ctgcccgcct tgctggaaga agagctgaac caggatatcg
    51241 atgaactgca tttcgccccg ttgaaaatcg accagcaact ggcaaccgta gctgtgattc
    51301 accagcagca tatgcgcaac attgcgcagt ggttgcaggc aaacggcatc acccgcgcta
    51361 ccgtcgcgcc agactggatg tccattcctt gtgggtttat ggctggcgat gcgcaacggg
    51421 ttatctgccg catcgatgaa tgccggggat ggagcgccgg gcgggcgctg gctccggtca
    51481 tgttccgcgc acagctcaat gagcagaatt taccgatttc actaaccgtg gtcggcattg
    51541 caccggaaga actgtctgca tgggctggtg cggacgccga acgcctgacc gttacggctc
    51601 tgcccgccat taccacttat ggcgaatcgg aagggaacct gctaacaggg ccgtggcagc
    51661 cccgcgtcag ctaccgaaaa cagtgggcgc gctggcgggt gatgattctg ccgatattgc
    51721 tgattctggt tgcgctggca gtggaacggg gcgtgacgtt atggagcgtc agcgaacagg
    51781 tggcgcaaag ccgcacccag gcggagaaac agttcttaac actgttcccg gagcagaagc
    51841 ggattgtgaa tttacgctct caggtgacga tggcgctgaa aaaatatcgc ccgcaggccg
    51901 acgatacccg gctgctcgca gaattgtcag cgatcgccag taccctgaaa tcagcgtcac
    51961 ttaccgacat cgaaatgcgt ggtttcacct ttgatcaaaa acgccagacg cttcacctcc
    52021 agctacgggc tgcgaacttt gccagcttcg acaaactgcg tagcgcactg gcggcagatt
    52081 atgttgtgca acaggacgcg ttacagaaag agggtgatgc ggtttccggc ggcgtaacgt
    52141 tgcggaggaa ataacatgtt acgcgataaa tttattctct attttcagca atggcgtgaa
    52201 cgccagttaa gccgtggcga acactggctg acacaacacc tggcggggcg ttctccgcgt
    52261 gaaaaaggca tgttactggc agcggtagtg ttcctgttta gcgccggata ttacgttctc
    52321 atctggcagc cgttgagcga acggattgag caacaggaga cgatgttgca gcagctggtg
    52381 gcgatgaacg cgcgactgaa gagcgccgcg ccggatatta ttgcggcgcg aaaatccggc
    52441 acgacaacgc cagcacaggt atcgcgggtc atcagcgaca gcgcttccgc gcactcagtg
    52501 gtcattaagc gaatagccga gcgtggggag aatattcagg tctggataga tccggtggtg
    52561 tttaatgacc tgctgaactg gttaaaagca ctggatgaaa aatatgcgct gcgggtgata
    52621 caaattgatg tcagcgcagg tgagaagcca gggatggtga atgtgcagcg actggaattt
    52681 ggacgggggt aggtattgcc atttccttaa ccccacctga taacccttag cccaatggtg
    52741 agtgcggaac tgtcaaaccc gctcaccggg ctgagtttcg atgtagcaaa aattatctcc
    52801 ctccagacca ccacggaata ttcggtaaca ctgaaggaac ctgtttttca ccatgtagcc
    52861 ttcgttaatc gacacctttc taagactgta aagatgctta agtgttttga cacattatga
    52921 ttattaatag ttgtaataaa tcattgagta acaattgata ggccaaaaca tataggataa
    52981 ttcttgtgtg atctgtattt tgtgtagcct ggaaattagt aaaattcctg gagataatca
    53041 gaaagggaat ttcaaataag cataataatt tccagtgaaa ctattcgtcg attttaagaa
    53101 atgtctgctc aggtatatct actgagggat ggtgttggtt gtaacactgg tcaaaataaa
    53161 tacacaaaat aatcaagatg tatattttaa tcgacgaaaa taattacctt cgggattatt
    53221 gatgcgactt aaataacacc atttaaatgt gatataaatc acaaatatgg ctgtaaagag
    53281 ggggctgtag atataaataa gaagtacacg agtgaatttt aaatagggaa atagtttctc
    53341 ggtgaataat ttattggtaa tcaatcgcgt gcgttctggt ttgaaacaga ttgcaggtat
    53401 ttttacgcat aaaatccatg ggggatatta taatcaagta gttaacagta ataacaaaaa
    53461 ataatttcct gagaaattaa ctctacattc taaaacggcc agttttctga aattaccaga
    53521 agacggaata cattcatgcc tgaagaatgg attagaagaa acgagacgga tctatttatc
    53581 cctgcggaaa taatttctgc tgaaattttt tgcgattatt aaaaaggtca aaccgtctga
    53641 gtaaatttta tccagttaca aataagcatt acctccagtg tattggtagc tgttaagcca
    53701 agggcggtag cgtacctgaa gagattagga tcacatcatc aaatggcaag aagtggattt
    53761 gaagtccaga aagtcaccgt agaggcatta tttctacgag aaatacgaac acgctttggt
    53821 aagttccgtc tgggatattt gtgggcgatt ctcgaaccct ctgcgcattt gctgatactg
    53881 ttgggcattt ttggttacat tatgcaccgc acgatgccag acatctcatt cccggtgttt
    53941 ttacttaatg gcctgattcc cttttttatc tttagcagta tcagcaatcg ttctgtaggt
    54001 gctattgaag cgaatcaggg gctgtttaat tatcgaccag taaaacccat cgatacgatc
    54061 attgcgcgtg cattgcttga gacactgatt tacgttgctg tttatatatt gcttatgctt
    54121 atcgtctgga tggcaggcga atatttcgaa ataacaaact ttttacaact tgtgcttacc
    54181 tggagtttgt taatcattct ttcatgtggc gttggcttaa tattcatggt cgttggtaaa
    54241 accttccctg aaatgcaaaa ggtcctgccg atactgctta agccgctgta tttcatctcc
    54301 tgcatcatgt tccctctaca ctcgattccg aagcaatact ggtcatatct actctggaat
    54361 ccattagtgc atgtcgtaga gttaagccgc gaggcagtta tgcctggcta tatcagcgaa
    54421 ggcgtgagtc tgaactacct tgcaatgttc actctggtca ccctgttcat cggtctggcg
    54481 ttataccgaa cgcgtgaaga ggcaatgctg acatcatgat taagattgaa aatttgacga
    54541 agtcatatcg cacgccaact ggtcgacact atgtgtttaa ggatttaaat attgaaatcc
    54601 cttcaggaaa aagtgttgcc tttattggtc gcaacggagc gggtaaatca acgttactga
    54661 gaatgattgg cggcattgac cgccccgaca gtggaaagat catcactaat aaaacaatat
    54721 catggccggt cggcctcgcc ggtggatttc agggaagttt aaccggacgc gaaaacgtaa
    54781 aatttgttgc gcggttatac gcgaagcaag aagaactgaa agagaaaatt gagtttgtcg
    54841 aagaatttgc cgagctcggt aagtacttcg atatgccgat taaaacttac tcatcaggta
    54901 tgcgatctcg tctgggcttt ggtttaagca tggcatttaa gtttgattat tatatcgttg
    54961 atgaagtaac cgcagtcggt gatgccaggt ttaaagaaaa atgcgctcaa ttgtttaaag
    55021 aaaggcataa agagtctagt tttttaatgg tttcacatag tttgaattca ttgaaagagt
    55081 tttgtgatgt ggcgctattt atatctagag atcatcagac ttgtctttat cagaatatta
    55141 atgaagcaat taagttatat caaaatgatg aaaatttgaa catttagtgg agctaaaaat
    55201 gcgtaaagtt atcacctttg gtacatttga tgtattgcat ataggtcata taaatatctt
    55261 aaaaagggca aaaaaaatgg gggactatct aatagttggc gtttcatccg attatttaaa
    55321 tttctcaaaa aaacagaggt atcctgttta tcctgaaact gagcgtcttg aaataattag
    55381 gtccttaaaa tttgtagatg aagtttttat tgaagagtca ttggaattaa agggcgagta
    55441 tattaaaaaa tttaaggcag atatcttagt gatgggagat gattggctag ggcgatttga
    55501 tgaatataag gaattatgtg aagtatcata tctgccaaga actgttggta tttcaacgac
    55561 agaactaatt tctcaaatca aaagatatgg ttaattttgg agaggaattt tgaaatttca
    55621 ttcagatata aaaagcgcta taaaaaatgc gaagaaattt aaaggtgttt tatttgttaa
    55681 tataatatct tatctgctgc taacaattgc gataagaaaa ggtcattcga aagagatttt
    55741 aaaatttaag tggttgattt tacttcttgg cgggagtgtc gctaaatatt ttgttgctag
    55801 ggcttcattt cttaatggtg attatatatg tgctaagaaa ttatatcaag agataattaa
    55861 ggtaaaccca atacatactg atagctatta tgggttggct gatgtttata gactaatcgg
    55921 aaataaatta gaagcgaagg gtatacttat tcaagttttt aatttttctg atcgtttgaa
    55981 aacttggcta ttattggcga acttggttga tacaaaagaa gattatgatg aattattgaa
    56041 tcattgggaa agggaatgtt cttataggaa tatacttaag tatcattatg atgtgaacga
    56101 ttaccttata attgctgcgt taagggctaa actatatgaa gaagcattaa atcgggttaa
    56161 agtattgttg ggaagtataa attctgaggc ttttatctta ccaaagacta gcaataaaaa
    56221 tttttctgtt aatgctgcta aaattgcatt attagatcta aaaaaaatat tggatactaa
    56281 taaaatacag ttttttttag ttagcggaac attacttggt tgtattcgtg ataaaaaatt
    56341 actaaaacat gacaaagata ttgatatagg agtatggagc gaaacaaatc tatccgcatt
    56401 atcaacgaaa attgcttgct ctggcttgtt tgatattgca cctatgcgct caccgtatac
    56461 gttgagaata aaacatgtta atggtgtggc aatcgacata ttttttcatt atcgagatca
    56521 tgacagctat tggcatgcgg gttcaaagct gagatggaac aatactccat ttaatttgat
    56581 ttcttatggt tttcttggta acgtgttttt aataccagaa aattatgatt tatatttgac
    56641 tgagaattat ggaaactgga tgcaagaaaa aatgaaattt gactctgcat tcgatacacc
    56701 caatcatgaa ataattaata tgtatgaatt gaaaatacac gcgtataaaa agctaataat
    56761 aaatctgaaa attggtagta ttgaaggtgt tagatattat gaagataaat taaatgaact
    56821 tgggtgtatt atatctaagc cttgaagcct atataactga atatattaaa tattaaaaat
    56881 aatcatggga acatagtatg ttgttaacta agttagctaa aaaaatatgc caatccaaag
    56941 aatatattaa cggaatttct ctatacaaaa aaaaagaatg ggagagggca ttattgttct
    57001 ttgagaaatc aatcataaaa agaacaaaac atgcggagag ctattttaaa gcaggaatat
    57061 gcaatctcaa attgcataga tatgaagcgg catttaaata tataagtaaa gcattagaat
    57121 tggaaccctc acatatacag tggaaagaac aactcgaaca gtgtgcaagg catcttgata
    57181 agttaaataa tcatatggtg agcaaatctt ctactgaaga agatttatta agggaaaaac
    57241 ttaatacaga ctttaataat ccgaaattac acgatagact tgctgagatt cttcataaaa
    57301 aagggcgttg gtggcaggaa gttgatacgc tgaaaaacgc aataaaatta gatccttcaa
    57361 tacctgagcg ttatttcaaa cttggcactg cattagaaaa gatggggcgt tttaatgagg
    57421 ctagtatata ttataaaaaa ggacttgaat ttaataaaaa acatgatgct atttggtatt
    57481 atgcactcgg atattcgctt gaatctgatg aaacaaacaa tgcagaaaat gtccgtagtt
    57541 ctgaaatagc atatgcgaca gctatatcgt tagacaaaaa attaaatagt aataaatttg
    57601 gaataggagc atttcatcaa tataagggac gctgggctaa ggctattgat gcgtatgaaa
    57661 aacatacatc cataaatcca ttatgtgcag agctatatta taggctcgga ttatcttatg
    57721 atagatgtta tcaatgggat aaagccgcag agaattatcg caaagcatta tcactcgatg
    57781 aaaatcatcc tcattggcat tataggctag ggtttgtttt agagagaagt caaaagtatc
    57841 ttgatgctgc tgttgcttat caatttgcag cacaaagcaa taccaaacat atctcgattt
    57901 ggtactatag atctggatac gcatatgcta aggctaataa atacaggcaa tcctgtgaaa
    57961 tgtatataaa agcatatgac tcttctcagg aaatttatgc atcacaaaca aaagctattg
    58021 ataacagtga tacccattta aactcttata gaaaaaaact taaatatgaa agcaggatta
    58081 ataccttaga gtcagcaatt tctaattcat gcataaatta tggattatgg tatgaattag
    58141 gtaaaacata tgaatcttca tgtaattgga atgatgctgt cattgcatat catgaaggta
    58201 ttatgagaaa caatatatat accaaagact attattatag aatcggatat tcattattcc
    58261 gcgatgggaa ttatcaacag gcatgtaatt tttttcgtaa aacagagatt tttggaacac
    58321 catatggggc tcctgatgat attctaacaa aaaatttaac ttttaggagg aatgctatat
    58381 acactgaatt ttatgaaaca ttatcaattg aaaaaaacac tatactttat gaaagctttc
    58441 atggtgcaag tatttcttgt aacccatatg cattattttt agatattatt gatgatcaac
    58501 gatttgataa tttcagacat atttgggtta tcaataatga aaaaaaaatc cctgagcatc
    58561 taaaaaataa aaaaaatgta tattttgttt caaggcaaag tgatttatac atgcaatgtt
    58621 tggcatcatg tgaatttttg ataaataatg ttagcttccc tgagtatttt ataagaaaga
    58681 aagggcagcg atatttaaat acttggcatg gaactcctat aaaatttctt ggaaaagata
    58741 ttaaagatga atttttagca cataaaaatg tggctagaaa ttttcttcac acaacacatt
    58801 tacttagtcc taatacacac acaactaata ttttattaga taggtatgat atatctaata
    58861 tatttagtgg ggaaattaaa gagttaggtt atcctcgcat tgaccggact attaatctta
    58921 gctctgaacg gaaggaatat atacgtagga aaattaatgc gaacgtttac gataaagtag
    58981 tcttgtatgc tccaacttgg agaggaatac atggaaaggc aactttagat atagaaaaac
    59041 taaagaatga tcttgaaaag ttagctgacc aggattgtca tatcgttttc cgtggtcatc
    59101 atatgatcga aaaattagta agtgagcaga atatttctgg tataacaatt gttccttctg
    59161 aggttgatac gaatgagtta ctaggggcga ttgatattct tatcacagat tattcaagta
    59221 ttgctttcga tttttttgta atgaatagac cagtaattta ctatgcgtat gacattgagc
    59281 aatataataa tgagagaggg ttgtatttcc ctctaaatga attacctgga accgtttgtt
    59341 ttaataatgt agagttactt aacgcgttaa gtggatatct tcgtaatgaa atatatttcg
    59401 atgcatctaa aggtgttgat aagttttgta aaaatgatga tggaagtgta tgtggaaaag
    59461 taattgaatg gttcttcttt gaagaaaaat ctatattgct caataaaaat aaaaaagaaa
    59521 atatactttt ttatattggt ccatttattc caaatggtat attgtcatca tggctaaacc
    59581 ttacatcggt aattgatcgt gataaatata atatttcttt ggctgttgac ccaaaatcaa
    59641 ttcatagttt tcaagaacgg ttcgagcagt ttaagagagt ctctccagat attcaagtca
    59701 ttggaacttg tggaaacatg ctttataata ttgaagagaa atggcttaat gataaattaa
    59761 ataatcaatt caccctggca agtaaggaaa tgtatgatat tattgatcat gcttaccaac
    59821 gagagttttt aaggctcttt ggctatagtc atattgatca tctaatccat tttgaaggtt
    59881 ataatcaatc ttgggttatc cggtttgcta atgctccgaa agatacagtt agaaataaaa
    59941 taatatttca gcataatgat aagttgagcg agtggagaga aaaatttcca tatcttagag
    60001 ttgtttttga tttttataaa tcttacaata aaattgtttc tgtttctgaa aaaacgatgg
    60061 agttgaacag agataactta agcgaatttt ttaatattga gcatgataag tttatttatt
    60121 gtgataatgt tcagaatcca gatgaggtga ttaaaaaatc tgatgatatt gatacaagtg
    60181 gatttaattt tgaaaatgat aaaatctact ttataacttt gggcagactt tcagtcgaaa
    60241 aagatcaaca aaagttgatt aatgcctttt gtagattaca aaaattatat cctaatattg
    60301 agttattaat tcttggagac ggccccctca agattgattt acaacgccag attaaaactt
    60361 taggactcga aaaatccgta catcttcttg gacgcatatc aaatcctttt ccactgctaa
    60421 agagagctga ctgttttgtc ctctcttcaa atcatgaagg gcagcctatg gttcttttcg
    60481 aagcaatgat tctagataaa cctataatat caactgacat tactggttct agaagtgctc
    60541 ttgaagggcg ttctggtgta ttagttgaaa atagtgtaga cggtctcttc aacggaatgc
    60601 gggactttat cttgggaagg ttggagttta aacattttga cattgaatct tatcaaaaaa
    60661 atgctttgtc gatgttttat gaaaaatgcc ttcattaaag cattaatcta tatcatgatc
    60721 gtcaagaacc attgattgat ttgtaaggag gtatacctcc ttacaaattt aaggaaattt
    60781 agttaagtta ataaataatt atgaataaga cagttttaca tattaatagt aacataacca
    60841 cagatgaaat tagtttattg ttgcaggcat ccaagtataa atatattcat tttgaagtaa
    60901 ttgggaaagg tgattcatgt aaaaatttaa ttgagttttc atatcatgat tataatctaa
    60961 aaaaacaatt aattgcttta actaatgcag gttttatgtc gtatgttcat cgtggaaatg
    61021 taacttcatt ggttcacttt gatgagataa aaaatctctg gattccagta aaagaaaaaa
    61081 agttttctat taattctgat gggattgtat atacgttaca acgggctgca tgcaaaataa
    61141 atgaaaagct actggttgtt ttctctcaga tgccaattga accatattct gcaagcttgt
    61201 acagatattt tgcaaaaaac ttttctacaa ttgataaata tgttggaaaa aatgtttcta
    61261 tattaagggt ggctgatatc ggaggaatta ctggctcatt ttacttaaat acaaatgcat
    61321 taccaaccaa tgctgataaa attaaaagtc ttatattaga agttattgaa caatgtcaaa
    61381 taaaatcaga tgatgtcgta ctttatggat gctcaaaagg aggtacggca gccgtttatc
    61441 atggattaac taataattat aaaatagtag tggtggatcc tatattaaat gatgaacatt
    61501 atgtaaataa taaaaatgat ctacatttaa tagagggtgt ttttcctcag ccaaaggaag
    61561 agttattcaa aaaagtcatt gatgactatt taataaacta taaggggaat atgtcttatt
    61621 ttattgtttc acaaaattca gaacagtaca aatatattat ggatattata aatccaatca
    61681 tcccttattc aacagttgca aatagcatca atgataaaat taaaagccat cctgatgttg
    61741 ggccacaatc aattcatatg attgtagcaa tgattaatat ggctctttct ggaataaaac
    61801 tatctaacgg catctgtgat tttgtttaaa taatatggct ttaagattaa attttgtttt
    61861 tatacttgcg gtgatttgca gtatccaaat ttgtagcgct gctattttaa gtaaaagcta
    61921 ttcacataat gatattcata tcactctgcc attattattt aatgagtacg atgatgtgct
    61981 cagtcagtat gacggggcaa aatatttata ccctagttca tttgctcttg atgatgataa
    62041 taatatttat attcagtata tttttgagcc aaaacaaaag aataatatat tggtttgtta
    62101 taataaaaaa ggaaattacc ttgggtacta taagatacca agtggtgggc gaggcggggc
    62161 cggcgaaggt ttggctatta tacaagacta taatgggttt aatgttgtat atgtcgtttc
    62221 gaatacggga accttgcaaa gatacaatat ggtgagtagc ttgtatggtg acagcttatt
    62281 taaggaaaga gatttttcaa ttaatgtttt taaccaattt tcgatgagtc aagattattg
    62341 gcttattgaa ggttatgaaa gcgaaagaag tataaagtat agtagaaata ggcttgaact
    62401 atattcagta agtggagatt tattaagagt aatttattta cagaataatt attctggata
    62461 tattgtatca gatgcgagtc ctgatgcaaa gaattataat aaaagacaag ggatagcttt
    62521 aggtgatggt ttttttgttg ctagttatgg tgctttttac tactccaaaa tggaaagtac
    62581 ttataataca tatcaaggca ttagagtttt tgatttttcc ggaaagcttt tatctgaaaa
    62641 aattttccct ccagatgaaa tgatgaatgt tctcaaaaaa atgggcttaa cagtttctag
    62701 aattgaaaat gagggagtga tagtcaagaa taatactatt tacagtatgt atatttatag
    62761 tgatagatac catcataaaa cctccagaag tgaagggatc attattctac tggataaatt
    62821 tactcaataa gaagtcagac gagtttttat tatataaaaa tgatatgttt acatggtaaa
    62881 ataattatat gaactaaata tataggaata tatctttatg agtcatataa ttataaaaac
    62941 aaggattctt gaagataatg cttttatttt acgttgataa tttagaataa attatgatat
    63001 tgactttgat ccaaacaaaa gatccttatt catacccaat gcattctcaa attttactcc
    63061 cccataataa accgcattaa cctgtgtctt aaccaataaa tacccccgaa acttcttaaa
    63121 cagtttcata tccggtttaa aatcggcctg ccagaactgg tgcaaatgcc cttgatacgt
    63181 caagcctttg atgtcataca gggcattgcc catcactttg agtggtttgt tatgaatcag
    63241 tgcagagatc cccgccgtac tgttaatcgt caccaccgct ttagcatggc gtaatagttc
    63301 cggcatcggg agatcgtgca cgtatatgac tcgctcacct aagccatatt ccttacttaa
    63361 ccgcttaatt aatggtcgat agagtctgtg accacgatcc atcggatgat gtttgatcac
    63421 caaataactt tctttcggcg ctttacgtga aaatgagtac ataacttcat taatatagtc
    63481 acgcacatcg ttataattac tgtggttacg aatctggcta tcgttatata cctgcaaaac
    63541 ggcaagataa taacgctggt ccagctcgtt cattaacctc ggtaatacct tacgctgtgt
    63601 taccttgtaa agttgcttgc gccagtatgc acgaacccag caacgtgctt cataccaggg
    63661 ggaaaacgat ttatggtggc ggtagcgagg gaactcatgg cggtaatgcc agcccatcag
    63721 gtaataccac atcgcatgac ctatacgttt catcgttgaa ggttttaagt tctcaacgtg
    63781 cggcgtaggc atatctggta acttacgata aaaatccgga tcgcgcggta gcgatgaata
    63841 tgcgtttact ccgccttctt caacggttat aaattgcggg cgtaaatatc cttcttcaaa
    63901 tgccagaaag cggagccctt tcgactttgc ccagcgtttt gcttctttat gcaatgggcg
    63961 acagtcacca aaacagagga tggtgtcaaa gtcatattgt cggtggagat ctcgcaacca
    64021 accaggaaac tctttcggcg tttggtagta agccagatat tgtcgatggc ggcagtaaaa
    64081 acgatcccca ccgttgaaga caacattcac agcgttacgg cctaatgact ctaaccattc
    64141 ggcgacgtca ttgaaaaaag gtcccattgg cccctgcaat agcagatatt ttttaccgga
    64201 caataaaatg gttagtgcat taccttgcat aaataatttg cgtaatagtt aactatgaaa
    64261 ttgtttagcc aaatctgacc ttgcagaaca taattaattt gcggtaatag cgtattaccc
    64321 gccccgcttt ttttcgggta ataaacatcg gctttcgcgg tgtcttgatt aaatattccg
    64381 cagcttcttc aaccgtaata ggttgtagcc gtgttgggtg gatataggtt ggataaacaa
    64441 tcaacgcctg atagatcaag tctgctatcg ttaatctgcg ctcgcggcgc gggcaatgat
    64501 gttcatcaac ggttaaaccc caactggcat agaagggcag gccgtaacaa tgaacttgct
    64561 tgccatgtaa taacgcttca aacccggaca atgatgtcat ggtgtgcact tcatctgcgc
    64621 gctgaatgca ttgaataata tctgcgtcca gtgcctgata atcagcgagt tcagcaatta
    64681 gttctgccgg aatattgccc ttgcgattcc ccaccagtac atccgggtgc ggtttataaa
    64741 taatgtaagc gtgcggattg cgctcgcgta ctgtgcgtaa taactcaagg ttgctcttaa
    64801 tcgagacagt gcctgtttta atagaggcat cgtcctctac ctgacccggc accaggataa
    64861 cttttttatc tttggcttcg gctggtagag agaaatcggc tcccaggttg tacttgctca
    64921 gtttactttc aaccagtcgc tggcgtaatt tttcagctcg catctgctgc gccagcgtta
    64981 gctggctatg attaagcagc acttccaggt cgctggggcg cgtggcgtca tagtagatcc
    65041 cgcgtttatc cagtaccaac gatagcggcg gcagcaggtc agagccaagt ccggatgaac
    65101 gcagaaaacc atcttccatt cgccataacg gcagtgattt tcgctgcgct tcggctcgcc
    65161 attgctgttc tccctttaca ccccatacca cgcaggcgct cgccgcagta cagcgacgtg
    65221 aaaaactcag ccggtttgtt gccgtttgca agaaaggttt caggatcgct gacttccaca
    65281 gcgttaagcc tagcgcccat aaataaccat cacgctgttg cagatgtcga cgttgtaatt
    65341 gcagccattg cagcacggta aatagatcgc ttacttctcc cgtttgcgga tcgatatagc
    65401 gacagtaacg caggtatgca gcggcaaaaa gttcctccag cgtggcagaa ccgcgtcggg
    65461 cagataacaa agcggactgt ggatgacgat cgtcggttaa gccccaacct gcataccagg
    65521 gctggccgaa acatgttact ggttttcctg ccagcaaggc ttcaaagccg tactgggagg
    65581 tcacgacgta aacctgggaa acgtgtcgca atagcgactg cgggctgaca ttctcggcaa
    65641 tcaaacgtac tcgttgcgtg gcgcgcagat cggcgaaata acctgttttc tttccttcca
    65701 ggacatccgg gtgcaccttc acccaaattt cggcttgagg attttccgcc atcgcggctt
    65761 ccagcatggc agcaaactca tgcgggccag cattgccata cgtcactgac atatcattaa
    65821 atgtctgatc gacaaccaga atgatgtctg cacgttctga ctcatcagcc acaaacgcag
    65881 gtgccaggtt atatttcgac aaatccccgg tcacgatggt gtgcatcgct tctctggcct
    65941 gatcagccaa tgccgcattt ccggctttat cctgtaccag tttctccagc gccgaaggct
    66001 tgctggcatc gtagtaaatg ccacaatcat ccaccaccag agaaagcggc ggctcgccat
    66061 tgacgccaag atccagcgaa cgcacaaatc catcttccag acgaatgacg ggttttcccg
    66121 ctgctttggc gatggcgact ggtttcgccg cgctgggacg atgtccccac acggcgatag
    66181 catcaacttc ttgcggaaca gggcgcagca gagaaagttt ctggcacggt tgcgccagaa
    66241 atttctccag atgcggaata cgccagatgc caggcgagta aatgccaatc atgcgttttc
    66301 agccagttcc tgtgccatca gggcgcgcac tttttccagg catgctgggg tgtcgacgcc
    66361 cggaccggtt gcggcaacct caaatgtgcg gatgttgatc cctgcgctca tcaaccgcag
    66421 ctgctccagt gattctgcct gctctggcat ggactccggt aactgactgt agttttgcag
    66481 cacatcgcga cgataagcgt aaataccaac gtgtttcagg tagcgcgctt tttcagcatt
    66541 acgcggatag ggaatcggcg aacggctgaa ataaagcgca tcctggcggg tatttaccac
    66601 cacttttacc gtgcttggct cggtcgcttc ttcggcagaa atcgcgtggc atagcgttgc
    66661 cactggcaac gcgggatcat cacgcattcc ttgtaacagc gtttctacat cccgcgggcg
    66721 aatcattggt tcatcgccct gcaggttaat gtagatatct gcctcgactt tatgcatcac
    66781 ctcgaccagc cgatcggtgc cggattcatg atcgttgcgc gtcatgatgg ctttcccgcc
    66841 aaacgcctgc acggcctttt caacacgcgg gtcgtctgtt gccacccaaa cttccgcaac
    66901 gcccgccacc tgtaacgcac gctcgtaaac atgctggatc atcggtttac caacaatatc
    66961 gagcagtggc ttacccggca ggcgcgagga gccatagcga gccggaatga caataactgc
    67021 tttgctcatt tcaactcctt acaaagacag aatcactttt gcacccaccg ccaactggta
    67081 aaggatggtg gaaataccgc gagtaacttc aatgtttttc gattcatatt tcggcagaac
    67141 cataatctca tcgcccggtt tgagtgaatc cacatcttct gcgttgacgg ctgcaccgtt
    67201 ctgacgaatg acgataattc tggcgttacc cgatttctgc gtcaggccac cacatttctc
    67261 gatgtaatcc tcggtggtca tacccttctg ccagctcacc gcgttcggga acagcacttc
    67321 gccatgaacc ataaccagcg atgttttctc cggaatattg atgacgtcgc catcttcaag
    67381 cagaacagaa tcaatattgg attcgttgag gatcacttca cctttcggaa caacggtgcg
    67441 tgctttcgcc acaaagcggc tgatcagttg tgcttcctgc attcgcaggc tggcttcttc
    67501 tttggtggat gactgggcag aaagtgatgc ttcctccagc ttttgcagcg agagattcag
    67561 catctctttc tgacgctgag ccactgatgg gcgataaagc tgaactgcgt tcatctgcga
    67621 catgctgttc gggcggactt tttccagaac cgcacgcata gtggaaccat aaggcagtac
    67681 catggcatgt tcaccggagt gtgcgccttc aacccgcacc tgaatggtgc cggcatagcg
    67741 gtcagtgctc acgattaagg tatcgccatt ttgcaacata cggcctggcg cagaactgat
    67801 cggatagtat tcgctgcgtt tttgcagccc ctgtttacgc ataatcgtaa tgtgagtcgc
    67861 gccaggctta gggcgcgccc agctcaacgc ttccgttacg ggaatgctgc tttcgcggaa
    67921 ctcaaagtcg tagctgttaa agacatcgcc ctgaacgctg aaagtatgct gacgcggccc
    67981 gacgatgatg gtgtcaccat cggcgaactg cgaaagcccc agtttgccgt tcagcaggaa
    68041 gtcgtacagg ttgacgttgg agcgcacgcg gttaccgcgc ttgaccacaa tatcaacgta
    68101 acttccgcgc tctggatcaa cgccgccagc cttgatcaga taattgagta acgaatcaga
    68161 cgtcacaccg ccatacagac caggattacg cacaaatccg gtcacgtaca cttttactgg
    68221 ctgcgcctgt aataaggagg cgtagacgtt gacgttggac tggtatactt ccttcacttt
    68281 ggatgtgacc agggcattta gctgactatt actgacgcca gcaactttca ccggaccaac
    68341 gttcggcagg aaaatattac ctttgggatc aacctgtaac gcaccatcaa aggtgaacgc
    68401 accccacaag cgaacctgaa tgctatcacc cggattcaga atatagtcag ggttgaatcc
    68461 taccgtcgca ccgctatctg cgctggtgcc gttgaaaagt tgagcaccaa acatacggct
    68521 cattaccacc ggcggtgcgg gcggcggggt attgtcgaag ccgctcatgt tttgcgtatc
    68581 cgactgttgc ccgttcagaa taccggtaag cggcgcggct cctgtaaggt ttgggtcggc
    68641 gttaatatca atggccgcgc tggcctgcgc cgcgtgacag gcggcaatca gtaaaattga
    68701 tttaaataat ttcatcacaa actcattcag cgacagcgtt agtctcggtg atcttcaata
    68761 acagccagca acagtttcag ggtgccgaat agcaggcagc acaccagtaa ccagcaggcg
    68821 atcaaataag ggatatttgg aaaagacgat tcctgcggca actgtggcga actgatcact
    68881 gacagcacct tgagcttacg agccgcttct acacgggtct tttcaatgga ggtcagggtc
    68941 agtttgtaca gctcggtgtt gaactctact tttgatttga tttcttcaaa atccaccgcc
    69001 atacggttta gcttgtcacc ctgcggcgct gtgattttgc ttttttcttc gtcaatttgt
    69061 gcctgcaatg actgaatcgc attacgcgca ctcacaactt gcggggcgtc ttcacgcaga
    69121 tacgtcagca agttccgcag gtccgcttcc atctggatct tctggcccat cagcgtattg
    69181 actaacgtgc tcgccgcctg tgcctgtgcc tgtggatcca gaacgttgtt gttgtcctga
    69241 taagagagca attccgcttt gctggcgtcc agacgctggc gtgccttttc catctccgtt
    69301 tctgcaaagg caagctggtc acgcgcgatg cgatgtgaca tctcattgat aaagcgctct
    69361 gactctttca gcacggtctg gttaaactta agcgcaaact ccgggctaaa gccctgcgtc
    69421 tgaatattca gtaatccggt tttatcgtca tacgagacgt tgatacggtc cttgtagtac
    69481 ttcaggaagc cttctgcagt ttcatccttg ctaagatgat taagaaaatc gagcccgctg
    69541 tggctaaacg cttcacgaaa atttagttgc ttatccagtg ccgccagcat atccggcgag
    69601 ttgatgtact ctttcagata caacgcatct tctgcggaac tggggttaga ggcacccaga
    69661 agcagaccaa aattcaggct gccgctgttt aaatcatccg agcgtttaat ggcgactttc
    69721 gactcgctca tatagcgagg ctggctgaag atagccagat agatcagcag caccgccatc
    69781 ggtgccagaa tgatgatttt cgccaggtgt ttttggatat ctgccagtga gatggcagac
    69841 agacgagcac gcatccagga tacggcagac ttcactttta tcaacatgta acgattatca
    69901 aagtttcagt ggggcgcaaa ctcaatgaga gactaacctc tcgcaccccg ttgcttaatt
    69961 agtcgaaaat gcgcacaagg ccagtgactt tatttgcctt gttggtcacc aataacgttg
    70021 agacgcggtg cttttgcatt ttttcttccg cttcaatgat catggtgtct tccggtagcg
    70081 tcagcggttc acgtgtcatc atctgcgcag ccgtggcgga tgtcagagaa tcctcttttt
    70141 ccataaagcg acgcaggtca ccgtcggtga taatgcccgc tagcccacct tctgcgtctt
    70201 ctaccatcac cattccctgg catccgctgg tgatacgttg aatcacggtt ttaaatgacg
    70261 catccagctg taccgcagga acatcatgct gcatgacatc agcaacgcgg gtcagcaggc
    70321 gacgacctaa tgaaccgccc gggtgatagc gcgcaaaatc attcggcata aatttgcgtt
    70381 ggtggatcat ggcaatcgcc agcgcatcgc cgatcgccat cgtcagcgtg gtagacgttg
    70441 ttggtgcaag attattcggg caggtttcat tcgccatgtg gagttccagc acggcatcag
    70501 catttttcgc cagcgtggaa tttccattat tggtaatggc gataattcgg ttgccgaaat
    70561 ttttcagtga aggaactagc ttgaggattt catccgtttc accgctggca gaaataagga
    70621 tcagaagatc gtaaggcgta atcatgccca gatcgccatg gaaagcttct gcaggatgaa
    70681 taaagaaact aggcgtaccc gtagaggcca gcgtcgctga cattttacga ccgacatgcc
    70741 ctgatttacc cattcccgaa agaataacgt gccctttaca gttcattatc aaattaagga
    70801 cacgctggta ctgcccggaa tccagttgtt tagacaagtt ttgtaatgct gcgccttgtt
    70861 ctgccagagt ctggcgaaca gaggtaatta gatatggatc gatagtactg ctctggtcat
    70921 caggtaaatg tctttcagac atatcagctc ctttgcacgg aaataatgcc acagtcacaa
    70981 caaacccgaa cccactataa tttatttatg ctaaatcaat gggttgtaac aaaattttta
    71041 atgaatataa aaccatggtc agtccatgct tatatgcagg acttatcacc ttcttattaa
    71101 gtcgcaatgt ctcatggagg tgcgaagatg ctgcaatatt acaggcgaga aggggaacca
    71161 aattttgccc acctatttaa cacaccccta cattatgaac aaggaattta ggatttttat
    71221 catttattaa aatgtttcct acttgactat taaaccaaaa aacaatttat caattgatta
    71281 tttccttgta aaaaaagaac gtatgaccat gttattaata aataaaacac gtaaataatg
    71341 gtgatgttgt tttgtgatta actatttaag agtagcataa taaacaagtt gacaatttct
    71401 atgtaaagaa gaagaaaata ataaaatttt agcggtgact attgaatgat atgatagttg
    71461 tggtgttgat tatttctggt aatgaatgcg gaattaaatc tggccggatt gtatttttgc
    71521 tatatctatt tttcaggaga ttattattaa atataaaaat aacaatggtg attatctccg
    71581 gttgacctgg agtcacgtat gccagcgagt gctttcttcc gggccttcct tcacttcaga
    71641 gaaaaagagg caatcatcag gctctttttg agtcatatgc gccgataatc aactgtcttg
    71701 aacgtaaaaa ttatggagca acatgatgct acgattcatt tcactcttcg ttctttctga
    71761 gcattgcgtt caggatggtc agcaatttac gcatactgtc aaccagcgcc acttttttgg
    71821 tttttccggc tgctgacaga tgcatgtaaa atgccgttat caccggatta aaccaggtta
    71881 ccacaagcgc tgccatgtgc agggctgctc ttgcctccgt acgcgcactg gcgtcgttcg
    71941 gccagcagat atgtgtggga taacagggat gttatgtgaa ctgaactgtg ttttgttagc
    72001 ggggggatgt ttattgatga ttcgggagtg tttaagacct gatggcgtta tgctcccaag
    72061 tttattttat ataaagatcc aaaagcaaaa acccgccttg tgggcgggtt ctttagaata
    72121 gtggtgccgg aatcggaatc gaaaatttct tttatcatat tgatattaat ataatttgtt
    72181 tggtttgatt aaaaatgtac cctcaattat acccgcagtc ccttttgata tcgaaaggga
    72241 atcacactcg ggatcaggct gagagcacag aaggaaagta cctggctatt atatcgttaa
    72301 ttctgtcaat cagttccagc cttttgaaag tgatgtgtgc agatcccttc ttaaagtatt
    72361 taatgatgaa catctcgtct tcatagcgct ctttaccctg aacggactgg atgtgaccat
    72421 ccagacgaat agtgatgttt tcccggttgt cggggatcgg tttgccgctg aacagatgta
    72481 gcattctttc caggtcgacg agtcggtcag tctgttgccc ggtgataaga tgaaaccccc
    72541 accggtccca cctcaccagg ttgttgacga taattttact gccaaatttg caggggcaat
    72601 tggttttgta attccagctc agtcccctga agacgttgat cacccctcgc tcaaacacct
    72661 catccttatt ctggtgtagt tgttcaaatg tgatcagaat gttcgcttca ctgatttccg
    72721 gaaaattatc gtactccagt gaccggtacc atgtatcacg ggcctgtgca tccattaagg
    72781 aaagcatccc tgaacgttgc atcaggtcac gccagatttc gcgatcgaga ttgcgtgtga
    72841 tggctttcat tgcggtttcc ggtttctcca tcagccagca gccgcagcga aaatcctgtt
    72901 tcatggccca gcccggggcg gctttaccgc caatactttc tgtcattgct gatatttcag
    72961 ccagttgccg gataagtgtg tcgatttgca gcagtgtact ctttcgtaca gccagaatgt
    73021 ggcggatatt ggttgagcag ataacgtccg tatgcccggt gaccacttcc ggacgggaga
    73081 ttgtgatgtc tgacattatc ggtttccctt ttggtttata aacgaaaacg c
//
LOCUS       DAMZFT010000009        70065 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.009, whole genome shotgun sequence.
ACCESSION   DAMZFT010000009 DAMZFT010000000
VERSION     DAMZFT010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 70065)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 70065)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..70065
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.009"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            120..812
                     /locus_tag="PN977_000694"
     CDS             120..812
                     /locus_tag="PN977_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HDI6040760.1"
                     /translation="MPLSAQQLAAQKNLSYVLAEKLAQRILKGEYEPGTILPGEIELG
                     EQFGVSRTAVREAVKTLTAKGMVLPRPRIGTRVMPQSNWNFLDQELLTWWMTEENFHQ
                     VIDHFLVMRICLEPQACLLAATVGTAEQKAHLNTLMAEMVALKENFRRERWIEVDMAW
                     HEHIYEMSANPFLTSFASLFHSVYHTYFTSITSDTVIKLDLHQAIVDAIIQSDGDAAF
                     KACQALLRSPDK"
     gene            835..2262
                     /gene="mdtD"
                     /locus_tag="PN977_000695"
     CDS             835..2262
                     /gene="mdtD"
                     /locus_tag="PN977_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter subunit MdtD"
                     /protein_id="HDI6040761.1"
                     /translation="MSDKKKRSMAGLPWIAAMAFFMQALDATILNTALPAIAHSLNRS
                     PLAMQSAIISYTLTVAMLIPVSGWLADRFGTRRIFTLAVSLFTLGSLACALSNSLPQL
                     VVFRVIQGIGGAMMMPVARLALLRAYPRNELLPVLNFVAMPGLVGPILGPVLGGVLVT
                     WATWHWIFLINIPIGIAGLLYARKHMPNFTTARRRFDITGFLLFGLSLVLFSSGIELF
                     GEKIVASWIALTVIVTSIGLLLLYILHARRTPNPLISLDLFKTRTFSIGIVGNIATRL
                     GTGCVPFLMPLMLQVGFGYQAFIAGCMMAPTALGSIIAKSMVTQVLRRLGYRHTLVGI
                     TVIIGLMIAQFSLQSPAMAIWMLILPLFILGMAMSTQFTAMNTITLADLTDDNASSGN
                     SVLAVTQQLSISLGVAVSAAVLRVYEGMEGTTTVEQFHYTFITMGIITVASAAMFMLL
                     KTTDGNNLIKRQRKSKPNRVPSESE"
     gene            complement(2228..3220)
                     /gene="rbsR"
                     /locus_tag="PN977_000696"
     CDS             complement(2228..3220)
                     /gene="rbsR"
                     /locus_tag="PN977_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose operon transcriptional repressor RbsR"
                     /protein_id="HDI6040762.1"
                     /translation="MATMKDVARLAGVSTSTVSHVINKDRFVSEAITAKVEAAIKELN
                     YAPSALARSLKLNQTHTIGMLITASTNPFYSELVRGVERSCFERGYSLVLCNTEGDEQ
                     RMNRNLETLMQKRVDGLLLLCTETHQPSREIMQRYPTVPTVMMDWAPFDGDSDLIQDN
                     SLLGGDLATQYLIDKGHTRIACITGPLDKTPARLRLEGYRAAMKRAGLNIPDGYEVTG
                     DFEFNGGFDAMRQLLSHPLRPQAVFTGNDAMAVGVYQALYQAELQVPQDIAVIGYDDI
                     ELASFMTPPLTTIHQPKDELGELAIDVLIHRITQPTLQQQRLQLTPILMERGSA"
     gene            complement(3224..4153)
                     /gene="rbsK"
                     /locus_tag="PN977_000697"
     CDS             complement(3224..4153)
                     /gene="rbsK"
                     /locus_tag="PN977_000697"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HDI6040763.1"
                     /translation="MQNAGSLVVLGSINADHILNLQSFPTPGETVTGNHYQVAFGGKG
                     ANQAVAAGRSGANIAFIACTGDDSIGESVRQQLATDNIDISPVSVIKGESTGVALIFV
                     NGEGENVIGIHAGANAALSPALVEAQRERIANASALLMQLESPLESVMAAAKIAHQNK
                     TIVALNPAPARELPDELLALVDIITPNETEAEKLTGIRVENDEDAAKAAQVLHEKGIR
                     TVLITLGSRGVWASVNGEGQRVPGFRVQAVDTIAAGDTFNGALITALLEEKPLPEAIR
                     FAHAAAAIAVTRKGAQPSVPWREEIDAFLDRQR"
     gene            complement(4279..5169)
                     /gene="rbsB"
                     /locus_tag="PN977_000698"
     CDS             complement(4279..5169)
                     /gene="rbsB"
                     /locus_tag="PN977_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter substrate-binding protein
                     RbsB"
                     /protein_id="HDI6040764.1"
                     /translation="MNMKKLATLVSAVALSATVSANAMAKDTIALVVSTLNNPFFVSL
                     KDGAQKEADKLGYNLVVLDSQNNPAKELANVQDLTVRGTKILLINPTDSDAVGNAVKM
                     ANQANIPVITLDRQATKGEVVSHIASDNVLGGKIAGDYIAKKAGEGAKVIELQGIAGT
                     SAARERGEGFQQAVAAHKFNVLASQPADFDRTKGLNVMQNLLTAHPDVQAVFAQNDEM
                     ALGALRALQTAGKSDVMVVGFDGTPDGEKAVNDGKLAATIAQLPDQIGAKGVETADKV
                     LKGEKVQAKYPVDLKLVVKQ"
     gene            complement(5194..6159)
                     /gene="rbsC"
                     /locus_tag="PN977_000699"
     CDS             complement(5194..6159)
                     /gene="rbsC"
                     /locus_tag="PN977_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002882531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter permease"
                     /protein_id="HDI6040765.1"
                     /translation="MTTQTVSGRRYFTKAWLMEQKSLIALLVLIAIVSTLSPNFFTIN
                     NLFNILQQTSVNAIMAVGMTLVILTSGIDLSVGSLLALTGAVAASIVGIEVNALVAVA
                     AALALGAAIGAVTGVIVAKGRVQAFIATLVMMLLLRGVTMVYTNGSPVNTGFTENADL
                     FGWFGIGRPLGVPTPVWIMGIVFLAAWYMLHHTRLGRYIYALGGNEAATRLSGINVNK
                     IKIIVYSLCGLLASLAGIIEVARLSSAQPTAGTGYELDAIAAVVLGGTSLAGGKGRIV
                     GTLIGALILGFLNNGLNLLGVSSYYQMIVKAVVILLAVLVDNKKQ"
     gene            complement(6164..7669)
                     /gene="rbsA"
                     /locus_tag="PN977_000700"
     CDS             complement(6164..7669)
                     /gene="rbsA"
                     /locus_tag="PN977_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter ATP-binding protein RbsA"
                     /protein_id="HDI6040766.1"
                     /translation="MEALLQLKGIDKAFPGVKALSGAALNVYPGRVMALVGENGAGKS
                     TMMKVLTGIYTRDAGTLLWLGKETTFTGPKSSQEAGIGIIHQELNLIPQLTIAENIFL
                     GREFVNRFGKIDWKTMYAEADKLLAKLNLRFKSDKLVGDLSIGDQQMVEIAKVLSFES
                     KVIIMDEPTDALTDTETESLFRVIRELKSQGRGIVYISHRMKEIFEICDDVTVFRDGQ
                     FIAEREVASLTEDSLIEMMVGRKLEDQYPHLDKAPGDIRLKVDNLCGPGVNDVSFTLR
                     KGEILGVSGLMGAGRTELMKVLYGALPRTSGYVTLDGHEVVTRSPQDGLANGIVYISE
                     DRKRDGLVLGMSVKENMSLTALRYFSRAGGSLKHADEQQAVSDFIRLFNVKTPSMEQA
                     IGLLSGGNQQKVAIARGLMTRPKVLILDEPTRGVDVGAKKEIYQLINQFKADGLSIIL
                     VSSEMPEVLGMSDRIIVMHEGHLSGEFTREQATQEVLMAAAVGKLNRVNQE"
     gene            complement(7677..8096)
                     /gene="rbsD"
                     /locus_tag="PN977_000701"
     CDS             complement(7677..8096)
                     /gene="rbsD"
                     /locus_tag="PN977_000701"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709562.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HDI6040767.1"
                     /translation="MKKGTVLNSDISSVISRLGHTDTLVVCDAGLPIPKSTTRIDMAL
                     TQGVPSFMQVLGVVTNEMQVEAAIIAEEIKQHNPQLHETLLTHLEQLQKHQGNTIEIR
                     YTTHEQFKQQTAESQAVIRSGECSPYANIILCAGVTF"
     gene            complement(8263..10131)
                     /gene="kup"
                     /locus_tag="PN977_000702"
     CDS             complement(8263..10131)
                     /gene="kup"
                     /locus_tag="PN977_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity potassium transporter Kup"
                     /protein_id="HDI6040768.1"
                     /translation="MSTDNKQSLPAITLAAIGVVYGDIGTSPLYTLRECLSGQFGFGV
                     ERDAVFGFLSLIFWLLIFVVSIKYLTFVMRADNAGEGGILTLMSLAGRNTSARTTSML
                     VIMGLIGGSFFYGEVVITPAISVMSAIEGLEIVAPQLDTWIVPLSIIVLTLLFMIQKH
                     GTAMVGKLFAPIMLTWFLILAGLGLRSIIANPEVLHALNPMWAVHFFLEYKTVSFIAL
                     GAVVLSITGVEALYADMGHFGKFPIRLAWFTVVLPSLTLNYFGQGALLLKNPEAIKNP
                     FFLLAPDWALIPLLIIAALATVIASQAVISGVFSLTRQAVRLGYLSPMRIIHTSEMES
                     GQIYIPFVNWMLYVAVVIVIVSFEHSSNLAAAYGIAVTGTMVLTSILSTTVARQNWHW
                     NKYFVALILIAFLCVDIPLFTANLDKLLSGGWLPLSLGTVMFIVMTTWKSERFRLLRR
                     MHEHGNSLEAMIASLEKSPPVRVPGTAVYMSRAINVIPFALMHNLKHNKVLHERVILL
                     TLRTEDAPYVHNVRRVQIEQLSPTFWRVVASYGWRETPNVEEVFHRCGLEGLSCRMME
                     TSFFMSHESLILGKRPWYLRLRGKLYLLLQRNALRAPDQFEIPPNRVIELGTQVEI"
     gene            10354..11850
                     /gene="ravA"
                     /locus_tag="PN977_000703"
     CDS             10354..11850
                     /gene="ravA"
                     /locus_tag="PN977_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418202.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA"
                     /protein_id="HDI6040769.1"
                     /translation="MAHPHLLAERISRLSSSLEKGLYERSHAIRLCLLAALSGESVFL
                     LGPPGIAKSLIARRLKFAFQNARAFEYLMTRFSTPEEVFGPLSIQALKDEGRYERLTS
                     GYLPEAEIVFLDEIWKAGPAILNTLLTAINERQFRNGAHIEKIPMRLLVAASNELPEA
                     DSSLEALYDRMLIRLWLDKVQDKANFRSMLTSQQDENDNPVPTSLQVTDEEYERWQKE
                     IGEITLPDHVFELIFMLRQQLDKLPDAPYVSDRRWKKAIRLLQASAFFSGRSAVAPVD
                     LILLKDCLWYDAQSLNLIQQQIDVLMTGHAWQQQGMLTRLGAIVQRHLQLQQQQSDKT
                     ALTVIRLGGIFSRRQQYQLPVNVTASTLTLLLQKPLKLHDMEVVHITFERSALEQWLS
                     KGGEIRGKLNGIGFAQKLNLEVDSTQHLVVRDVSLQGSTLALPGSSAEGLPGEIKQQL
                     EELESDWRKQHALFSEQQKCLFIPGDWLGRIEASLQDVGAQIRQAQQC"
     gene            11844..13295
                     /gene="viaA"
                     /locus_tag="PN977_000704"
     CDS             11844..13295
                     /gene="viaA"
                     /locus_tag="PN977_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA stimulator ViaA"
                     /protein_id="HDI6040770.1"
                     /translation="MLTLDTLNVMLAVSEEGLIEEMIIALLASPQLAVFFEKFPRLKA
                     AITDDVPRWREALRSRLKDARVPPELTEEVMCYQQSQLLSTPQFIVQLPQILDLLHRL
                     NSPWAEQARQLVDANSTITSALHTLFLQRWRLSLIVQATTLNQQLLEEEREQLLSEVQ
                     ERMTQSGQLEPILADNNTAAGRLWDMSAGQLKRGDYQLIVKYGEFLNEQPELKRLAEQ
                     LGRSREAKSIPRNDAQMETFRTLVREPATVPEQVDGLQQSDDILRLLPPELATLGITE
                     LEYEFYRRLVEKQLLTYRLHGESWREKVIERPVVHKDYDEQPRGPFIVCVDTSGSMGG
                     FNEQCAKAFCLALMRIALAENRRCYIMLFSTEIVRYELSGPQGIEQAIRFLSQRFRGG
                     TDLASCFRAIMERLQSREWFDADAVVISDFIAQRLPDDVTSKVKELQRVHQHRFHAVA
                     MSAHGKPGIMRIFDHIWRFDTGMRSRLLRRWRR"
     gene            complement(13300..14292)
                     /gene="asnA"
                     /locus_tag="PN977_000705"
     CDS             complement(13300..14292)
                     /gene="asnA"
                     /locus_tag="PN977_000705"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HDI6040771.1"
                     /translation="MKTAYIAKQRQISFVKSHFSRQLEERLGLIEVQAPILSRVGDGT
                     QDNLSGCEKAVQVKVKALPDAQFEVVHSLAKWKRQTLGQHDFSAGEGLYTHMKALRPD
                     EDRLSPLHSVYVDQWDWERVMADGERQFSTLKSTVEAIWAGIKATEAAVSEEFGLAPF
                     LPDQIHFVHSQELLSRYPNLDAKGRERAIAKDLGAVFLVGIGGKLSDGHRHDVRAPDY
                     DDWSTPSELGYAGLNGDILVWNPVLEDAFELSSMGIRVDADTLKHQLALTGDEDRLQL
                     EWHQALLRGEMPQTIGGGIGQSRLTMLLLQLPHIGQVQCGVWSAAVRESVPSLL"
     gene            14444..14902
                     /gene="asnC"
                     /locus_tag="PN977_000706"
     CDS             14444..14902
                     /gene="asnC"
                     /locus_tag="PN977_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator AsnC"
                     /protein_id="HDI6040772.1"
                     /translation="MENYLIDNLDRGILEALMENARTAYAELAKQFGVSPGTIHVRVE
                     KMKQAGIITGARIDVSPKQLGYDVGCFIGIILKSAKDYPSALAKLESLDEVTEAYYTT
                     GHYSIFIKVMCRSIDALQHVLINKIQTIDEIQSTETLIVLQNPIMRTIKP"
     gene            14992..15435
                     /gene="mioC"
                     /locus_tag="PN977_000707"
     CDS             14992..15435
                     /gene="mioC"
                     /locus_tag="PN977_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein MioC"
                     /protein_id="HDI6040773.1"
                     /translation="MADITLISGSTLGGAEYVAEHLAEKLEEAGFTTETLHGPLLEEL
                     SASGIWLVISSTHGAGDIPDNLSPFYEALQEQKPDLSAVRFGAIGIGSREYDTFCGAI
                     DKLEAELKNSGAKQTGETLKINILDHDIPEDPAEEWLGSWINLLK"
     gene            15814..17703
                     /gene="mnmG"
                     /locus_tag="PN977_000708"
     CDS             15814..17703
                     /gene="mnmG"
                     /locus_tag="PN977_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000499793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HDI6040774.1"
                     /translation="MFYPDPFDVIIIGGGHAGTEAAMAAARMGQQTLLLTHNIDTLGQ
                     MSCNPAIGGIGKGHLVKEVDALGGLMAKAIDLAGIQFRILNASKGPAVRATRAQADRV
                     LYRQAVRTALENQPNLMIFQQAVEDLIVENDRVVGAVTQMGLKFRAKAVVLTVGTFLD
                     GKIHIGLDNYSGGRAGDPPSIPLSRRLRELPLRVGRLKTGTPPRIDARTIDFSVLAQQ
                     HGDNPMPVFSFMGNASQHPQQVPCYITHTNEKTHDVIRSNLDRSPMYAGVIEGVGPRY
                     CPSIEDKVMRFADRNQHQIFLEPEGLTSNEIYPNGISTSLPFDVQMQIVRSMQGMENA
                     KIVRPGYAIEYDFFDPRDLKPTLESKFIQGLFFAGQINGTTGYEEAAAQGLLAGLNAA
                     RLSADKEGWAPARSQAYLGVLVDDLCTLGTKEPYRMFTSRAEYRLMLREDNADLRLTE
                     IGRELGLVDDERWARFNEKLENIERERQRLKSTWVTPSAEAAAEVNAHLTAPLSREAS
                     GEDLLRRPEMTYEKLTTLTPFAPALTDEQAAEQVEIQVKYEGYIARQQDEIEKQLRNE
                     NTLLPATLDYRQVSGLSNEVIAKLNDHKPASIGQASRISGVTPAAISILLVWLKKQGM
                     LRRSA"
     gene            17767..18390
                     /gene="rsmG"
                     /locus_tag="PN977_000709"
     CDS             17767..18390
                     /gene="rsmG"
                     /locus_tag="PN977_000709"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HDI6040775.1"
                     /translation="MLNKLSLLLKDAGISLTDHQKNQLIAYVNMLHKWNKAYNLTSVR
                     DPNEMLVRHILDSIVVAPYLQGERFIDVGTGPGLPGIPLSIVRPEAHFTLLDSLGKRV
                     RFLRQVQHELKLENIEPVQSRVEEFPSEPPFDGVISRAFASLNDMVSWCHHLPGEQGR
                     FYALKGQMPEDEIALLPEEYQVESVVKLHVPALDGERHLVMIKANKI"
     gene            19007..19387
                     /gene="atpI"
                     /locus_tag="PN977_000710"
     CDS             19007..19387
                     /gene="atpI"
                     /locus_tag="PN977_000710"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418195.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit I"
                     /protein_id="HDI6040776.1"
                     /translation="MSVSLVSRNVARKLLLVQLLVVIASGLLFSLKDPFWGVSAISGG
                     LAVFLPNVLFMIFAWRHQAHTPAKGRVAWTFAFGEAFKVLAMLVLLVVALAVLKAVFL
                     PLIVTWVLVLVVQILAPAVINNKG"
     gene            19396..20211
                     /gene="atpB"
                     /locus_tag="PN977_000711"
     CDS             19396..20211
                     /gene="atpB"
                     /locus_tag="PN977_000711"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HDI6040777.1"
                     /translation="MASENMTPQDYIGHHLNNLQLDLRTFSLVDPHNPPATFWTINID
                     SMFFSVVLGLLFLVLFRSVAKKATSGVPGKFQTAIELVIGFVNGSVKDMYHGKSKLIA
                     PLALTIFVWVFLMNLMDLLPIDLLPYIAEHVLGLPALRVVPSADVNVTLSMALGVFIL
                     ILFYSIKMKGIGGFTKELTLQPFNHWAFIPVNLILEGVSLLSKPVSLGLRLFGNMYAG
                     ELIFILIAGLLPWWSQWILNVPWAIFHILIITLQAFIFMVLTIVYLSMASEEH"
     gene            20258..20497
                     /gene="atpE"
                     /locus_tag="PN977_000712"
     CDS             20258..20497
                     /gene="atpE"
                     /locus_tag="PN977_000712"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004246596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HDI6040778.1"
                     /translation="MENLNMDLLYMAAAVMMGLAAIGAAIGIGILGGKFLEGAARQPD
                     LIPLLRTQFFIVMGLVDAIPMIAVGLGLYVMFAVA"
     gene            20559..21029
                     /gene="atpF"
                     /locus_tag="PN977_000713"
     CDS             20559..21029
                     /gene="atpF"
                     /locus_tag="PN977_000713"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001052215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HDI6040779.1"
                     /translation="MNLNATILGQAIAFVLFVLFCMKYVWPPLMAAIEKRQKEIADGL
                     ASAERAHKDLDLAKASATDQLKKAKAEAQVIIEQANKRRSQILDEAKAEAEQERTKIV
                     AQAQAEIEAERKRAREELRKQVAILAVAGAEKIIERSVDEAANSDIVDKLVAEL"
     gene            21044..21577
                     /gene="atpH"
                     /locus_tag="PN977_000714"
     CDS             21044..21577
                     /gene="atpH"
                     /locus_tag="PN977_000714"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HDI6040780.1"
                     /translation="MSEFITVARPYAKAAFDFAVEHQSVERWQDMLAFAAEVTKNEQM
                     AELLSGALAPETLAESFIAVCGEQLDENGQNLIRVMAENGRLNALPDVLEQFIHLRAV
                     SEATAEVDVISAAALSEQQLAKISAAMEKRLSRKVKLNCKIDKSVMAGVIIRAGDMVI
                     DGSVRGRLERLADVLQS"
     gene            21590..23131
                     /gene="atpA"
                     /locus_tag="PN977_000715"
     CDS             21590..23131
                     /gene="atpA"
                     /locus_tag="PN977_000715"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001176751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HDI6040781.1"
                     /translation="MQLNSTEISELIKQRIAQFNVVSEAHNEGTIVSVSDGVIRIHGL
                     ADCMQGEMISLPGNRYAIALNLERDSVGAVVMGPYADLAEGMKVKCTGRILEVPVGRG
                     LLGRVVNTLGAPIDGKGPLDHDGFSAVEAIAPGVIERQSVDQPVQTGYKAVDSMIPIG
                     RGQRELIIGDRQTGKTALAIDAIINQRDSGIKCIYVAIGQKASTISNVVRKLEEHGAL
                     ANTIVVVATASESAALQYLAPYAGCAMGEYFRDRGEDALIIYDDLSKQAVAYRQISLL
                     LRRPPGREAFPGDVFYLHSRLLERAARVNAEYVEAFTKGEVKGKTGSLTALPIIETQA
                     GDVSAFVPTNVISITDGQIFLETNLFNAGIRPAVNPGISVSRVGGAAQTKIMKKLSGG
                     IRTALAQYRELAAFSQFASDLDDATRKQLDHGQKVTELLKQKQYAPMSVAQQSLVLFA
                     AERGYLADVELSKIGSFEAALLAYVDRDHAPLMQEINQTGGYNDEIEGKLKGILDSFK
                     ATQSW"
     gene            23182..24045
                     /gene="atpG"
                     /locus_tag="PN977_000716"
     CDS             23182..24045
                     /gene="atpG"
                     /locus_tag="PN977_000716"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HDI6040782.1"
                     /translation="MAGAKEIRSKIASVQNTQKITKAMEMVAASKMRKSQDRMAASRP
                     YAETMRKVIGHLAHGNLEYKHPYLEDRDVKRVGYLVVSTDRGLCGGLNINLFKKLLAE
                     MKTWTDKGVQCDLAMIGSKGVSFFNSVGGNVVAQVTGMGDNPSLSELIGPVKVMLQAY
                     DEGRLDKLYIVSNKFINTMSQVPTISQLLPLPASDDDDLKHKSWDYLYEPDPKALLDT
                     LLRRYVESQVYQGVVENLASEQAARMVAMKAATDNGGSLIKELQLVYNKARQASITQE
                     LTEIVSGAAAV"
     gene            24072..25454
                     /gene="atpD"
                     /locus_tag="PN977_000717"
     CDS             24072..25454
                     /gene="atpD"
                     /locus_tag="PN977_000717"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017800291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HDI6040783.1"
                     /translation="MATGKIVQVIGAVVDVEFPQDAVPRVYDALEVQNGNERLVLEVQ
                     QQLGGGIVRTIAMGSSDGLRRGLDVKDLEHPIEVPVGKATLGRIMNVLGEPVDMKGEI
                     GEEERWAIHRAAPSYEELSNSQELLETGIKVIDLMCPFAKGGKVGLFGGAGVGKTVNM
                     MELIRNIAIEHSGYSVFAGVGERTREGNDFYHEMTDSNVIDKVSLVYGQMNEPPGNRL
                     RVALTGLTMAEKFRDEGRDVLLFVDNIYRYTLAGTEVSALLGRMPSAVGYQPTLAEEM
                     GVLQERITSTKTGSITSVQAVYVPADDLTDPSPATTFAHLDATVVLSRQIASLGIYPA
                     VDPLDSTSRQLDPLVVGQEHYDTARGVQSILQRYQELKDIIAILGMDELSEEDKLVVA
                     RARKIQRFLSQPFFVAEVFTGSPGKYVSLKDTIRGFKGIMEGEYDHLPEQAFYMVGSI
                     EEAVEKAKKL"
     gene            25475..25894
                     /gene="atpC"
                     /locus_tag="PN977_000718"
     CDS             25475..25894
                     /gene="atpC"
                     /locus_tag="PN977_000718"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001251972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HDI6040784.1"
                     /translation="MAMTYHLDVVSAEQQMFSGLVEKIQVTGSEGELGIYPGHAPLLT
                     AIKPGMIRIVKQHGHEEFIYLSGGILEVQPGNVTVLADTAIRGQDLDEARAMEAKRKA
                     EEHISSSHGDVDYAQASAELAKAIAQLRVIELTKKAM"
     gene            26243..27613
                     /gene="glmU"
                     /locus_tag="PN977_000719"
     CDS             26243..27613
                     /gene="glmU"
                     /locus_tag="PN977_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HDI6040785.1"
                     /translation="MLNNAMSVVILAAGKGTRMYSDLPKVLHTLAGKAMVQHVIDAAN
                     ELGAAHVHLVYGHGGDLLKQALKDDNLNWVLQAEQLGTGHAMQQAAPFFADDEDILML
                     YGDVPLISVETLQRLRDAKPQGGIGLLTVKLDDPTGYGRITRENGKVTGIVEHKDATD
                     EQRQIQEINTGILIANGADMKRWLAKLTNNNAQGEYYITDIIALAYQEGREIVAVHPQ
                     RLSEVEGVNNRLQLSRLERVYQSEQAEKLLLAGVMLRDPARFDLRGTLTHGRDVEIDT
                     NVIIEGNVTLGHRVKIGTGCVIKNSVIGDDCEISPYTVVEDANLAAACTIGPFARLRP
                     GAELLEGAHVGNFVEMKKARLGKGSKAGHLTYLGDAEIGDNVNIGAGTITCNYDGANK
                     FKTIIGDDVFVGSDTQLVAPVTVGKGATIAAGTTVTRNVGENALAISRVPQTQKEGWR
                     RPVKKK"
     gene            27775..29604
                     /gene="glmS"
                     /locus_tag="PN977_000720"
     CDS             27775..29604
                     /gene="glmS"
                     /locus_tag="PN977_000720"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HDI6040786.1"
                     /translation="MCGIVGAIAQRDVAEILLEGLRRLEYRGYDSAGLAVVDAEGHMT
                     RLRRLGKVQMLAQAAEEHPLHGGTGIAHTRWATHGEPSEANAHPHVSEHIVVVHNGII
                     ENHEPLREELKARGYTFVSETDTEVIAHLVNWELKQGGTLREAVLRAIPQLRGAYGTV
                     IMDSRHPDTLLAARSGSPLVIGLGMGENFIASDQLALLPVTRRFIFLEEGDIAEITRR
                     SVNIFDKTGAEVKRQDIESNLQYDAGDKGIYRHYMQKEIYEQPNAIKNTLTGRISHGQ
                     VDLSELGPNADELLSKVEHIQILACGTSYNSGMVSRYWFESLAGIPCDVEIASEFRYR
                     KSAVRRNSLMITLSQSGETADTLAGLRLSKELGYLGSLAICNVPGSSLVRESDLALMT
                     NAGTEIGVASTKAFTTQLTVLLMLVAKLSRLKGLDASIEHDIVHGLQALPSRIEQMLS
                     QDKRIEALAEDFSDKHHALFLGRGDQYPIALEGALKLKEISYIHAEAYAAGELKHGPL
                     ALIDADMPVIVVAPNNELLEKLKSNIEEVRARGGQLYVFADQDAGFVSSDNMHIIEMP
                     HVEEVIAPIFYTVPLQLLAYHVALIKGTDVDQPRNLAKSVTVE"
     gene            29912..30484
                     /locus_tag="PN977_000721"
     CDS             29912..30484
                     /locus_tag="PN977_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbria major subunit LpfA-O113"
                     /protein_id="HDI6040787.1"
                     /translation="MKRNIIGGAFTLASLMLAGHALAEDGVVHFVGEIVDTTCEVTSD
                     TADQTVPLGKVSKNAFSGVGSLASPQQFSIKLEKCPATYTQAAVRFDGTEAPGGDGDL
                     KVGTPLTAGNPGDFTGTGQAIAATGVGIRIFNQSDNSQVKLYNDSAYTAIDAEGKAEM
                     KFIARYVATNATVTAGTANADSQFTVEYKK"
     gene            30532..31263
                     /locus_tag="PN977_000722"
     CDS             30532..31263
                     /locus_tag="PN977_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001572122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
                     /protein_id="HDI6040788.1"
                     /translation="MSRKSCLIRLFKTTVSCKLFTAIILSVFLVQPALTYAGVVIGGT
                     RVVYLSNNPDKSISVFSKEEKIPYLIQAWVDPFNKEDKSKAPFTVIPPVSRLEPAQEK
                     VLRIIHTKGVSLPDDRESVFWLNIKNIPPSASNKAANSLEIAVKTRIKLFWRPASIRL
                     IPEDAAPKVKWHREGRNLIAENPNPIHISVMDVIVDGHDVPLNMLRPFETLTLPLPAN
                     SAGGQMIWRFINDYGAVSDPIKMTL"
     gene            31288..33810
                     /locus_tag="PN977_000723"
     CDS             31288..33810
                     /locus_tag="PN977_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HDI6040789.1"
                     /translation="MMTTRIKVGLTAGTCLIFSQSLMAEVSVFNPALLEIDHQSGVDI
                     RQFNRANLMPPGVYSVDIFINGKMFERQDVTFVQDNPDADLHACFVAIKKTLTTFGVK
                     VDALKSLNDVDETVCIDPGPRIEGSSWQFDSDKLQLNISIPQIYMDAMAYDYISPSRW
                     DEGINALTINYDFSGSHTLRSDYGSQETDTSYLNLRNGLNIGPWRLRNYSTLNTTDGS
                     AEYNSISTWIQRDIAALRSQIMIGDTWTASDIFDSTQIRGARLYTDNDMLPASQNGFA
                     PVVRGIAKSNATVIIRQNGYVIYQSAVPQGAFEITDLNTASTGGDLDVTIKEEDGSEQ
                     RFTQPYASLAILKREGQTDVDVSVGELRDEDGFTPDVIQAQILHGFPYGFTLYGGMQA
                     AEKYGSAALGVGKDLGALGAISFDVTHARAKFSHDDTETGQSYRFLYSKRFDDTDTSL
                     RLVGYRYSTEGYYTLNEWASRRNNPEDFWETGNRRSRVEGTLTQSLGRDYGNLYLTLS
                     RQQYWHTDDVERLMQFGYSSSWKRLSWNVSWSYSNTARQGTGNNHASDNTSEQIYMLS
                     LSVPLSGWWGNSYATYSVSQNDNSGSSHQLGLSGTALERNNLSWNLMQSYNSHDDEVG
                     GNMSLTYDGTYGTVNGSYNYSQNSQRLNYGIRGGILAHSEGVTLSQELGETIALVKAP
                     GAAGLEIDNMRGAATDWRGYTVKTQLNPYDENRVAISDNYFSKSNIELDNTVVTMVPT
                     RGAVVKAEFVTRVGYRVLFRVAGTKGKPAPFGAIATVQNTSSADSGIVGDLGELYLSG
                     LPEKGQVMLSWGENAATTCTFDYSISIPESESGLIEQGVTCH"
     gene            33821..34891
                     /locus_tag="PN977_000724"
     CDS             33821..34891
                     /locus_tag="PN977_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001685709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HDI6040790.1"
                     /translation="MNKYIIQWCFTVFIFSFSGATFAAPKGICTSDNGAFHSTLDFSG
                     YLITADENRVGETFNKTVTNGDSYPAHCHCDTGKVGEFPYIYYTARINEALSYAGVRS
                     NVNYFNLNPNLDVGISIDLLGVGYINAPFEYHANRPTGGSYKCSRTDPLSISSGAKAI
                     IYFYIKKTFAGKLIVPETLVAKLYGTISRDTPVDYSQPMADVYIRGDITAPQSCEINS
                     LRPINFDFKEIPAADFSSVAGSTVTTHKITKTVTIECENLGILNTDDISTSFYATEPS
                     TDNSMVVTSNPNVGIKIYDKNNKEINVNGGELPTDMGKSNVFGEKAGSVTFSAAPASL
                     TGARPAPGTFTATATITIEIVR"
     gene            35139..36179
                     /gene="pstS"
                     /locus_tag="PN977_000725"
     CDS             35139..36179
                     /gene="pstS"
                     /locus_tag="PN977_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HDI6040791.1"
                     /translation="MKVMRTTVATVVAATLSMSAFSVFAEASLTGAGATFPAPVYAKW
                     ADTYQKETGNKVNYQGIGSSGGVKQIIANTVDFGASDAPLSDEKLAQEGLFQFPTVIG
                     GVVLAVNIPGLKSGELVLDGKTLGDIYLGKIKKWDDEAIAKLNPGLKLPSQNIAVVRR
                     ADGSGTSFVFTSYLAKVNEEWKNNVGTGSTVKWPIGLGGKGNDGIAAFVQRLPGAIGY
                     VEYAYAKQNNLAYTKLISADGKPVSPTEENFANAAKGADWSKTFAQDLTNQKGEDAWP
                     ITSTTFILIHKDQKKPEQGVEVLKFFDWAYKTGAKQANDLDYASLPDSVVEQVRAAWK
                     TNIKDSSGKPLY"
     gene            36266..37225
                     /gene="pstC"
                     /locus_tag="PN977_000726"
     CDS             36266..37225
                     /gene="pstC"
                     /locus_tag="PN977_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008806729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstC"
                     /protein_id="HDI6040792.1"
                     /translation="MAATKPAFNPPGKKGDIIFSVLVKLAALIVLLMLGGIIVSLIIS
                     SWPSIQKFGLAFLWTKGWDAPNDIYGALVPIYGTLVTSFIALLIAVPVSFGIALFLTE
                     LAPGWLKRPLGIAIELLAAIPSIVYGMWGLFIFAPLFAVYFQEPVGNIMSNIPIVGAL
                     FSGPAFGIGILAAGVILAIMIIPYIAAVMRDVFEQTPVMMKESAYGIGCTTWEVIWRI
                     VLPFTKNGVIGGIMLGLGRALGETMAVTFIIGNTYQLDSASLYMPGNSITSALANEFA
                     EAESGLHVAALMELGLILFVITFIVLAASKFMIMRLAKNEGAR"
     gene            37225..38115
                     /gene="pstA"
                     /locus_tag="PN977_000727"
     CDS             37225..38115
                     /gene="pstA"
                     /locus_tag="PN977_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HDI6040793.1"
                     /translation="MAMVEMQTTAALAESRRKMQARRRLKNRIALTLSMATMAFGLFW
                     LIWILMSTITRGIDGMSLALFTEMTPPPNTEGGGLANALAGSGLLILWATVFGTPLGI
                     MAGIYLAEYGRKSWLAEVIRFINDILLSAPSIVVGLFVYTIVVAQMEHFSGWAGVIAL
                     ALLQVPIVIRTTENMLKLVPDSLREAAYALGTPKWKMISAITLKASVSGIMTGILLAI
                     ARIAGETAPLLFTALSNQFWSTDMMQPIANLPVTIFKFAMSPFAEWQQLAWAGVLIIT
                     LCVLLLNILARVVFAKNKHG"
     gene            38205..38978
                     /gene="pstB"
                     /locus_tag="PN977_000728"
     CDS             38205..38978
                     /gene="pstB"
                     /locus_tag="PN977_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000063125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HDI6040794.1"
                     /translation="MSMVETAPSKIQVRNLNFYYGKFHALKNINLDIAKNQVTAFIGP
                     SGCGKSTLLRTFNKMFELYPEQRAEGEILLDGDNILTNSQDIALLRAKVGMVFQKPTP
                     FPMSIYDNIAFGVRLFEKLSRADMDERVQWALTKAALWNETKDKLHQSGYSLSGGQQQ
                     RLCIARGIAIRPEVLLLDEPCSALDPISTGRIEELITELKQDYTVVIVTHNMQQAARC
                     SDHTAFMYLGELIEFSNTDDLFTKPAKKQTEDYITGRYG"
     gene            38993..39718
                     /gene="phoU"
                     /locus_tag="PN977_000729"
     CDS             38993..39718
                     /gene="phoU"
                     /locus_tag="PN977_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HDI6040795.1"
                     /translation="MDSLNLNKHISGQFNAELESIRTQVMTMGGMVEQQLSDAITAMH
                     NQDSDLAKRVIEGDKNVNMMEVAIDEACVRIIAKRQPTASDLRLVMVISKTIAELERI
                     GDVADKICRTALEKFSQQHQPLLVSLESLGRHTIQMLHDVLDAFARMDIDEAVRIYRE
                     DKKVDQEYEGIVRQLMTYMMEDSRTIPSVLTALFCARSIERIGDRCQNICEFIFYYVK
                     GQDFRHVGGDELDKLLAGKDPKE"
     gene            40145..40292
                     /locus_tag="PN977_000730"
                     /pseudo
     CDS             40145..40292
                     /locus_tag="PN977_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_307391.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            40259..42634
                     /locus_tag="PN977_000731"
     CDS             40259..42634
                     /locus_tag="PN977_000731"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="type III effector protein"
                     /protein_id="HDI6040796.1"
                     /translation="MSLDDVGKYSVTHKDITNILAGDSQSFLKRSLWEFILDLFPMSH
                     IKEAKQAIVDFVTKTDDKVEMFNRVKSLADQDQQWRFNTTTEFTVDENKDIIVSRVFN
                     ININAQDYHNDAEKNTMRSLFSEELSLDIYKHDIHFDQKAFQSGMLKIQFPNDNEKLS
                     VYLKNKIPFENITIDLSALEKEALASLKYCNFKKVTFTGAISYENLNGSVFGNCFFED
                     CIFENVSLVSSRYRVKPNNEIPIYGVFKECFLYRCEIKNFNIDTSKIYSVNQDPNVGK
                     PVGAYLFMQSFVDECTLQDGVCKNASVIASSLLCCKIAKLNGAEMDFIGTSFYHKYKC
                     ELKYDNHFYNCDFSSSDMTYKRNGIRKDLLDFDPREYFKKDGGKLNLDKRISDLIYIN
                     LFPKMSGGRYINDKYVAGDIEKYLKLSNCNLLETCLGESINGITLNDCAIDHLTTWLS
                     GNLAANTQIRIPVYMDMKGAIAENGISDFMKNLMEINSWVEEFNNHGKDAESYYQKEL
                     LYLQSLLVKLGRFNLDDEGKKYQLNGEAIQYIILKVLYNIEKDIANEIDIDKVNFMHK
                     LYTGFFWQRPYDQRNYLKINRSAISNSKTFHFHFDFNDLRNIYKEYNKNHKDDSFDKM
                     QKIFLALNEFKERTKINYRDYRKVSKYVDDKVDLFSGIEQYLREVIEKNNSYNKENYT
                     AKKQKEADLFMLRNNLVKTKAKLSEESCMLNKEDKKDAAKIRKINETLNKIDDEIATI
                     DKEIVKLKDETERLEKEYEQENTLNDVVQNIRSWLKENQNMVKAIKKIDTE"
     gene            complement(43104..44291)
                     /locus_tag="PN977_000732"
     CDS             complement(43104..44291)
                     /locus_tag="PN977_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000369293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III effector protein"
                     /protein_id="HDI6040797.1"
                     /translation="MDNLIGTPPNHAVPHKYIDMEQMEYLCHLNRFSKLSDDFLINGP
                     RDDLFTYKYVLDGSFSNLQHLLPKGQLQKIQQRLSSLIDKNKFHCFHIFLLKLCSIEN
                     IPVPNADYAFFDDEMPFTLTDEQIENISFLNAYHKEKKGNNDIVTFDFMSYDHHHNFS
                     TTIALTNDSFHISSINNHNAQVIFDENVLLYTNELPESSQWCYQLIKNMISLHCRYNN
                     NFKVKKTCSFDFKNKSFCHSSLQYITFIACLFSHANMKCSTFHDINFDMCEIKNCNFD
                     NSEMNFISCVGTNFSGSTFNNVKTTTAQLIKTPTKWTNNTLKYWFSSSNKRNIIFTLN
                     TISDRDIKLKCIKDILLSLVDQKANIYSVRQELLDFLNNDLYKNDGEILSYKESIMLF
                     CAV"
     gene            44592..47000
                     /locus_tag="PN977_000733"
     CDS             44592..47000
                     /locus_tag="PN977_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III effector"
                     /protein_id="HDI6040798.1"
                     /translation="MVAKLKPDVFVNTNPFLEAMYKERLSHKGYSDKIALSINDKKYN
                     VNSKDIENILDGKGDLFKKRTLWEFVRDLFPGSHIKEVKGLIYEFVTKVDNKAEVFDK
                     IKSLAKKEQQWRFSTKTDFTTNENNEVIVSRSFNLYTGATSNDNEKKQVSSERLTLDN
                     YIDDLHFDNSPLKRFTFDDYAVKLTTLIKNKIPIINTTINLSSLSKDVLNSLKYCSFK
                     NVIFSGVINSPNLEGPVFENCYFEDCKFNNIQLYETNDDTVGSGNKKPIKGMFKGCFI
                     SKCKIENYRCETSKIYTVTQPDSIQEKLGSYLFMQSFVQDCIIQGGRCPGSSILLSHF
                     YNCTIAKLDAHGMDFLANAFNKSGDNETRAQDQGTVFDCCNLNSIKMNCGLGNVSSDE
                     YKFNPEEYFSDYIERRSRNLKLDDNFSNCVENIEKAVTTSFSKENSENDNISFNCCDL
                     RRANLDNYYREDLCKDCSIDPDSIYLEDGVTPIKYIYMNLDGAISKDQIPNFLKKVSA
                     INSDVKDFYNTELKIKEEYNNAFSDLESFLSTLYVENKSYKEKYHFNNGRAEFFIFKD
                     MQYKAQNIITNMIESDRIKFIESIIKKIIPPADVKMPTKDSDKFIRQKQHESHNQFDI
                     DYKLNYGDLVPLFDGVEEKQIEVLKIQLENIKNFKAYYDSELNKFASDFRNFSGAFAI
                     NWEDLLKNYQEIRASIKDYDDLLREIKELTISRDKSLEEKSTLFDNKRDKWNSQEVQD
                     EVKALNKKIADCDNEIRSNLNIVQNFRRENQYKANKKISNAMLNILDWFERYPDIVKN
                     ITQA"
     gene            complement(47137..47802)
                     /gene="yieH"
                     /locus_tag="PN977_000734"
     CDS             complement(47137..47802)
                     /gene="yieH"
                     /locus_tag="PN977_000734"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconate phosphatase"
                     /protein_id="HDI6040799.1"
                     /translation="MSQIEAVFFDCDGTLVDSEVICSRAYVTMFREFGIHVDLEEIFT
                     RFKGVKLYEIIDIISKEQGVTMIQADAEHVYRAEVARLFDTELEDIAGANELLASINV
                     PMCVVSNGPINKMQHSLGKLKMLHYFPEKLFSGYDIQRWKPDPALMFHAAKAMNVNVE
                     NCILVDDSSAGAQSGIDAGMEVFYFCADPHNKPIVHPKVTTFTELAQLPALWKARGWN
                     ITR"
     gene            47969..49306
                     /gene="adeP"
                     /locus_tag="PN977_000735"
     CDS             47969..49306
                     /gene="adeP"
                     /locus_tag="PN977_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeP"
                     /protein_id="HDI6040800.1"
                     /translation="MSQQHTTQASGQGMLERVFKLREHGTTARTEVIAGFTTFLTMVY
                     IVFVNPQILGVAGMDTSAVFVTTCLIAAFGSIMMGLFANLPVALAPAMGLNAFFAFVV
                     VQAMGLPWQVGMGAIFWGAIGLLLLTIFRVRYWMIANIPVSLRVGITSGIGLFIGMMG
                     LKNAGVIVANPETLVSIGNLTSHSVLLGILGFFIIAILASRNIHAAVLVSIVVTTLLG
                     WMLGDVHYNGIVSAPPSVMTVVGHVDLAGSFNLGLAGVIFSFMLVNLFDSSGTLIGVT
                     DKAGLADDKGKFPRMKQALYVDSISSVTGSFIGTSSVTAYIESSSGVSVGGRTGLTAV
                     VVGLLFLLVIFLSPLAGMVPGYAAAGALIYVGVLMTSSLARVNWQDLTESVPAFITAV
                     MMPFSFSITEGIALGFISYCVMKIGTGRLRDLSPCVIIVALLFILKIVFIDAH"
     gene            complement(49360..49926)
                     /gene="yieF"
                     /locus_tag="PN977_000736"
                     /gene_synonym="chrR"
     CDS             complement(49360..49926)
                     /gene="yieF"
                     /locus_tag="PN977_000736"
                     /gene_synonym="chrR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I chromate reductase YieF"
                     /protein_id="HDI6040801.1"
                     /translation="MSEKLQVVTLLGSLRKGSFNGMVARTLPKIAPASMEVNALPSIA
                     DIPLYDADVQQEDGFPATVEALAEQIRQADGVVIVTPEYNYSVPGGLKNAIDWLSRLP
                     DQPLAGKPVLIQTSSMGVIGGARCQYHLRQILVFLDAMVMNKPEFMGGVIQNKVDPQT
                     GEVIDQGTLDHLTGQLTAFGEFIQRVKI"
     gene            complement(49948..50697)
                     /locus_tag="PN977_000737"
     CDS             complement(49948..50697)
                     /locus_tag="PN977_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HDI6040802.1"
                     /translation="MATHFARGILTEGHLISVRLPSQCHQEARNIPPHRQSRFLASRG
                     LLAELMFMLYGIGELPEIVTLPKGKPVFSDKNLPSFSISYAGNMVGVALTTEGECGLD
                     MELQRATRGFHSPHAPDNHTFSSNESLWISKQNDPNEARAQLITLRRSVLKLTGDVLN
                     DDPRDLQLLPIAGRLKCAHVNHVEALCDAEDVLVWSVSVTPTIEKLSVWELDGKHGWK
                     SLPDIHSRANNPTSRMMRFAQLSTVKAFSPY"
     gene            complement(50854..51813)
                     /gene="yidZ"
                     /locus_tag="PN977_000738"
     CDS             complement(50854..51813)
                     /gene="yidZ"
                     /locus_tag="PN977_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator YidZ"
                     /protein_id="HDI6040803.1"
                     /translation="MKKSITTLDLNLLLCLQLLMQERSVTKAAKRMNVTPSAVSKSLA
                     KLRAWFDDPLFVNSPLGLSPTPLMVSMEQNLAEWMQMSNLLLDKPHHQTPRGLKFELA
                     AESPLMMIMLNALSKRIYQRYPQATIKLRNWDYDSLDAITRGEVDIGFSGRESHPRSR
                     ELLSSLPLAIDYEVLFSDVPCVWLRKDHPALHEAWNLDTFLRYPHISICWEQSDTWAL
                     DNVLQELGRERTIAMSLPEFEQSLFMAAQPDNLLLATAPRYCQYYNQLHQLPLVALPL
                     PFDEIQQKKLEVPFTLLWHKRNSRNPKIVWLRETIKNLYASMA"
     gene            complement(51788..52963)
                     /gene="mdtL"
                     /locus_tag="PN977_000739"
     CDS             complement(51788..52963)
                     /gene="mdtL"
                     /locus_tag="PN977_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtL"
                     /protein_id="HDI6040804.1"
                     /translation="MSRFLICSFALVLLYPAGIDMYLVGLPRIAADLNASEAQLHIAF
                     SVYLAGMAAAMLFAGKVADRSGRKPVAIPGAALFIIASVFCSLAETSTLFLAGRFLQG
                     LGAGCCYVVAFAILRDTLDDRRRAKVLSLLNGITCIIPVLAPVLGHLIMLKFPWQSLF
                     WTMATMGIAVLMLSLFILKETRPAAPAASDKPRENSESLLNRFFLSRVVITTLSVSVI
                     LTFVNTSPVLLMEIMGFERGEYATIMALTAGVSMTVSFSTPFALGIFKPRTLMITSQV
                     LFLAAGITLAVSPSHAVSLFGITLICAGFSVGFGVAMSQALGPFSLRAGVASSTLGIA
                     QVCGSSLWIWLAAVVGIGAWNMLIGILIACSIVSLLLIMFVAPGRPVAAHEEIHHHA"
     gene            complement(53096..54343)
                     /gene="tnaB"
                     /locus_tag="PN977_000740"
                     /pseudo
     CDS             complement(53096..54343)
                     /gene="tnaB"
                     /locus_tag="PN977_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312673.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity tryptophan permease TnaB"
     gene            complement(54434..55849)
                     /gene="tnaA"
                     /locus_tag="PN977_000741"
     CDS             complement(54434..55849)
                     /gene="tnaA"
                     /locus_tag="PN977_000741"
                     /EC_number="4.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709490.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase"
                     /protein_id="HDI6040805.1"
                     /translation="MENFKHLPEPFRIRVIEPVKRTTRAYREEAIIKSGMNPFLLDSE
                     DVFIDLLTDSGTGAVTQSMQAAMMRGDEAYSGSRSYYALAESVKNIFGYQYTIPTHQG
                     RGAEQIYIPVLIKKREQEKGLDRSKMVAFSNYFFDTTQGHSQINGCTVRNVYIKEAFD
                     TGVRYDFKGNFDLEGLERGIEEVGPNNVPYIVATITSNSAGGQPVSLANLKAMYSIAK
                     KYDIPVVMDSARFAENAYFIKQREAEYKDWTIEQITRETYKYADMLAMSAKKDAMVPM
                     GGLLCVKDDSFFDVYTECRTLCVVQEGFPTYGGLEGGAMERLAVGLYDGMNLDWLAYR
                     IAQVQYLVDGLEEIGVVCQQAGGHAAFVDAGKLLPHIPADQFPAQALACELYKVAGIR
                     AVEIGSFLLGRDPKTGKQLPCPAELLRLTIPRATYTQTHMDFIIEAFKHVKENAANIK
                     GLTFTYEPKVLRHFTAKLKEV"
     gene            complement(56070..56144)
                     /gene="tnaC"
                     /locus_tag="PN977_000742"
     CDS             complement(56070..56144)
                     /gene="tnaC"
                     /locus_tag="PN977_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase leader peptide"
                     /protein_id="HDI6040806.1"
                     /translation="MNILHICVTSKWFNIDNKIVDHRP"
     gene            complement(56386..57750)
                     /gene="mnmE"
                     /locus_tag="PN977_000743"
     CDS             complement(56386..57750)
                     /gene="mnmE"
                     /locus_tag="PN977_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HDI6040807.1"
                     /translation="MSDNDTIVAQATPPGRGGVGILRISGLKAREVAETVLGKLPKPR
                     YADYLPFKDADGSVLDQGIALWFPGPNSFTGEDVLELQGHGGPVILDLLLKRILTIPG
                     LRIARPGEFSERAFLNDKLDLAQAEAIADLIDASSEQAARSALNSLQGAFSARVNHLV
                     EALTHLRIYVEAAIDFPDEEIDFLSDGKIEAQLNDVIADLDAVRAEARQGSLLREGMK
                     VVIAGRPNAGKSSLLNALAGREAAIVTDIAGTTRDVLREHIHIDGMPLHIIDTAGLRE
                     ASDEVERIGIERAWQEIEQADRVLFMVDGTTTDAVDPAEIWPEFIARLPAKLPITVVR
                     NKADITGETLGMSEVNGHALIRLSARTGEGVDVLRNHLKQSMGFDTNMEGGFLARRRH
                     LQALEQAAEHLQQGKAQLLGAWAGELLAEELRLAQQNLSEITGEFTSDDLLGRIFSSF
                     CIGK"
     gene            complement(57856..59502)
                     /gene="yidC"
                     /locus_tag="PN977_000744"
     CDS             complement(57856..59502)
                     /gene="yidC"
                     /locus_tag="PN977_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HDI6040808.1"
                     /translation="MDSQRNLLVIALLFVSFMIWQAWEQDKNPQPQAQQTTQTTTTAA
                     GSAADQGVPASGQGKLISVKTDVLDLTINTRGGDVEQALLPAYPKELNSTQPFQLLET
                     SPQFIYQAQSGLTGRDGPDNPANGPRPLYNVEKDAYVLAEGQNELQVPMTYTDAAGNT
                     FTKTFVLKRGDYAVNVNYNVQNAGEKPLEISTFGQLKQSITLPPHLDTGSSNFALHTF
                     RGAAYSTPDEKYEKYKFDTIADNENLNISSKGGWVAMLQQYFATAWIPHNDGTNNFYT
                     ANLGNGIAAIGYKSQPVLVQPGQTGAMNSTLWVGPEIQDKMAAVAPHLDLTVDYGWLW
                     FISQPLFKLLKWIHSFVGNWGFSIIIITFIVRGIMYPLTKAQYTSMAKMRMLQPKIQA
                     MRERLGDDKQRISQEMMALYKAEKVNPLGGCFPLLIQMPIFLALYYMLMGSVELRQAP
                     FALWIHDLSAQDPYYILPILMGVTMFFIQKMSPTTVTDPMQQKIMTFMPVIFTVFFLW
                     FPSGLVLYYIVSNLVTIIQQQLIYRGLEKRGLHSREKKKS"
     gene            complement(59505..59762)
                     /gene="yidD"
                     /locus_tag="PN977_000745"
     CDS             complement(59505..59762)
                     /gene="yidD"
                     /locus_tag="PN977_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HDI6040809.1"
                     /translation="MAPPLSPGSRVLIALIRVYQRLISPLLGPHCRFTPTCSSYGIEA
                     LRRFGVIKGSWLTVKRVLKCHPLHPGGDDPVPPGPFDTREH"
     gene            complement(59726..60085)
                     /gene="rnpA"
                     /locus_tag="PN977_000746"
     CDS             complement(59726..60085)
                     /gene="rnpA"
                     /locus_tag="PN977_000746"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014172301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HDI6040810.1"
                     /translation="MVKLAFPRELRLLTPSQFTFVFQQPQRAGTPQITILGRLNSLGH
                     PRIGLTVAKKNVRRAHERNRIKRLTRESFRLRQHELPAMDFVVVAKKGVADLDNRALS
                     EALEKLWRRHCRLARGS"
     gene            complement(60102..60242)
                     /gene="rpmH"
                     /locus_tag="PN977_000747"
     CDS             complement(60102..60242)
                     /gene="rpmH"
                     /locus_tag="PN977_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001686914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HDI6040811.1"
                     /translation="MKRTFQPSVLKRNRSHGFRARMATKNGRQVLARRRAKGRARLTV
                     SK"
     gene            complement(60472..60552)
                     /locus_tag="PN977_000748"
     CDS             complement(60472..60552)
                     /locus_tag="PN977_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6040812.1"
                     /translation="MTIDKNWLNRSNKDPGRSLRFTRQPV"
     gene            60849..62252
                     /gene="dnaA"
                     /locus_tag="PN977_000749"
     CDS             60849..62252
                     /gene="dnaA"
                     /locus_tag="PN977_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HDI6040813.1"
                     /translation="MSLSLWQQCLARLQDELPATEFSMWIRPLQAELSDNTLALYAPN
                     RFVLDWVRDKYLNNINGLLTSFCGADAPQLRFEVGTKPVTQTPQAAVTSNVAAPAQVA
                     QTQPQRAAPSTRSGWDNVPAPAEPTYRSNVNVKHTFDNFVEGKSNQLARAAARQVADN
                     PGGAYNPLFLYGGTGLGKTHLLHAVGNGIMARKPNAKVVYMHSERFVQDMVKALQNNA
                     IEEFKRYYRSVDALLIDDIQFFANKERSQEEFFHTFNALLEGNQQIILTSDRYPKEIN
                     GVEDRLKSRFGWGLTVAIEPPELETRVAILMKKADENDIRLPGEVAFFIAKRLRSNVR
                     ELEGALNRVIANANFTGRAITIDFVREALRDLLALQEKLVTIDNIQKTVAEYYKIKVA
                     DLLSKRRSRSVARPRQMAMALAKELTNHSLPEIGDAFGGRDHTTVLHACRKIEQLREE
                     SHDIKEDFSNLIRTLSS"
     gene            62257..63357
                     /gene="dnaN"
                     /locus_tag="PN977_000750"
     CDS             62257..63357
                     /gene="dnaN"
                     /locus_tag="PN977_000750"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006177590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HDI6040814.1"
                     /translation="MKFTVEREHLLKPLQQVSGPLGGRPTLPILGNLLLQVADGTLSL
                     TGTDLEMEMVARVALVQPHEPGATTVPARKFFDICRGLPEGAEIAVQLEGERMLVRSG
                     RSRFSLSTLPAADFPNLDDWQSEVEFTLPQATMKRLIEATQFSMAHQDVRYYLNGMLF
                     ETEGEELRTVATDGHRLAVCSMPIGQSLPSHSVIVPRKGVIELMRMLDGGDNPLRVQI
                     GSNNIRAHVGDFIFTSKLVDGRFPDYRRVLPKNPDKHLEAGCDLLKQAFARAAILSNE
                     KFRGVRLYVSENQLKITANNPEQEEAEEILDVTYSGAEMEIGFNVSYVLDVLNALKCE
                     NVRMMLTDSVSSVQIEDAASQSAAYVVMPMRL"
     gene            63357..64430
                     /gene="recF"
                     /locus_tag="PN977_000751"
     CDS             63357..64430
                     /gene="recF"
                     /locus_tag="PN977_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HDI6040815.1"
                     /translation="MSLTRLLIRDFRNIETADLALSPGFNFLVGANGSGKTSVLEAIY
                     TLGHGRAFRSLQIGRVIRHEQEAFVLHGRLQGEERETAIGLTKDKQGDSKVRIDGTDG
                     HKVAELAHLMPMQLITPEGFTLLNGGPKYRRAFLDWGCFHNEPGFFTAWSNLKRLLKQ
                     RNAALRQVTRYEQLRPWDKELIPLAEQISTWRAEYSAGIAADMADTCKQFLPEFSLTF
                     SFQRGWEKETEYAEVLERNFERDRQLTYTAHGPHKADLRIRADGAPVEDTLSRGQLKL
                     LMCALRLAQGEFLTRESGRRCLYLIDDFASELDDERRGLLASRLKATQSQVFVSAISA
                     EHVIDMSDENSKMFTVEKGKITD"
     gene            64459..66873
                     /gene="gyrB"
                     /locus_tag="PN977_000752"
     CDS             64459..66873
                     /gene="gyrB"
                     /locus_tag="PN977_000752"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HDI6040816.1"
                     /translation="MSNSYDSSSIKVLKGLDAVRKRPGMYIGDTDDGTGLHHMVFEVV
                     DNAIDEALAGHCKEIIVTIHADNSVSVQDDGRGIPTGIHPEEGVSAAEVIMTVLHAGG
                     KFDDNSYKVSGGLHGVGVSVVNALSQKLELVIQREGKIHRQIYEHGVPQAPLAVTGET
                     EKTGTMVRFWPSLETFTNVTEFEYDILAKRLRELSFLNSGVSIRLRDKRDGKEDHFHY
                     EGGIKAFVEYLNKNKTPIHPNIFYFSTEKDGIGVEVALQWNDGFQENIYCFTNNIPQR
                     DGGTHLAGFRAAMTRTLNAYMDKEGYSKKAKVSATGDDAREGLIAVVSVKVPDPKFSS
                     QTKDKLVSSEVKSAVEQQMNELLAEYLLENPTDAKIVVGKIIDAARAREAARRAREMT
                     RRKGALDLAGLPGKLADCQERDPALSELYLVEGDSAGGSAKQGRNRKNQAILPLKGKI
                     LNVEKARFDKMLSSQEVATLITALGCGIGRDEYNPDKLRYHNIIIMTDADVDGSHIRT
                     LLLTFFYRQMPEIVERGHVYIAQPPLYKVKKGKQEQYIKDDEAMDQYQISIALDGATL
                     HTNASAPALAGEALEKLVSEYNATQKMINRMERRYPKAMLKELIYQPTLTETDLSDEQ
                     TVTRWVNALVSELNDKEQHGSQWKFDVHTNAEQNLFEPIVRVRTHGVDTDYPLDHEFI
                     TGGEYRRICTLGEKLRGLLEEDAFIERGERRQPVASFEQALDWLVKESRRGLSIQRYK
                     GLGEMNPEQLWETTMDPESRRMLRVTVKDAIAADQLFTTLMGDAVEPRRAFIEENALK
                     AANIDI"
     gene            67017..67415
                     /locus_tag="PN977_000753"
     CDS             67017..67415
                     /locus_tag="PN977_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF937 domain-containing protein"
                     /protein_id="HDI6040817.1"
                     /translation="MGLFDDVVGAFLKGDAGKYQAILSWVEEQGGIQVLLEKLQSGGL
                     GDILSTWLSNQQGNQPVSGEQLESALGTNAVSDLGQKLGVDTSTASSLLAEQLPKIID
                     ALSPQGEVSAQANNDLLSAGMELLKGKLFR"
     gene            67530..68342
                     /gene="yidA"
                     /locus_tag="PN977_000754"
     CDS             67530..68342
                     /gene="yidA"
                     /locus_tag="PN977_000754"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HDI6040818.1"
                     /translation="MAIKLIAIDMDGTLLLPDHTISPAVKNAIAAARARGVNVVLTTG
                     RPYAGVHNYLKELHMEQPGDYCITYNGALVQKAADGSTVAQTALSYDDYRFLEKLSRE
                     VGSHFHALDRTTLYTANRDISYYTVHESFVATIPLVFCEAEKMDPNTQFLKVMMIDEP
                     AILDQAIARIPQEVKEKYTVLKSAPYFLEILDKRVNKGTGVKSLADVLGIKPEEIMAI
                     GDQENDIAMIEYAGVGVAMDNAIPSVKEVANFVTKSNLEDGVAFAIEKYVLN"
     gene            complement(68386..69042)
                     /locus_tag="PN977_000755"
     CDS             complement(68386..69042)
                     /locus_tag="PN977_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010377884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6040819.1"
                     /translation="MKLNLKGFFKAASLCSLAFALTGCITWGLVSNTASRPHAEQWRS
                     DTIKGLSLAEDSNGTKGYVFVGESLDYLLTTGGDEVVKMLNDPAIHGERITVSDNAKF
                     ILSSSDTNFSGSITLYYDWDNEEDKALATQYGFICNTQRCTWALDGLKGSIHQKNKKA
                     DYSNVMVFHQPFTVGFYKYKATDGVPRGLANALLPVTLTLDIVTSPLQFLILCTTRNC
                     "
     gene            69321..70010
                     /gene="dgoR"
                     /locus_tag="PN977_000756"
     CDS             69321..70010
                     /gene="dgoR"
                     /locus_tag="PN977_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-galactonate utilization transcriptional
                     regulator DgoR"
                     /protein_id="HDI6040820.1"
                     /translation="MTLNKTDRIVITLGKQIVHGKYVPGSPLPAEAELCEEFATSRNI
                     IREVFRSLMAKRLIEMKRYRGAFVAPRNQWNYLDTDVLQWVLENDYDPRLISAMSEVR
                     NLVEPAIARWAAERATSSDLAQIESALNEMIANNQDREAFNEADIRYHEAVLQSVHNP
                     VLQQLSIAISSLQRAVFERTWMGDEANMPQTLQEHKALFDAIRHQDGDAAEQAALTMI
                     ASSTRRLKEIT"
BASE COUNT        18238 a        16793 c        17517 g        17517 t
ORIGIN      
        1 tttttcagca aaagccccgt taatgtgcca taaaacaagc aatttgccgt ttttgcaacc
       61 gtaatcacac ttcctggtgg catactaagg aggaataaaa agaaaaagga agattagtaa
      121 tgcctttaag cgcacaacag ttagctgcac agaaaaacct ttcgtatgtt ctggctgaga
      181 agctggcgca acgcatctta aaaggtgaat atgaacccgg caccattttg cctggtgaga
      241 ttgagctggg cgagcaattt ggagtgagtc gtacagcggt acgcgaagcg gtcaaaacgt
      301 taacggcaaa agggatggtt ttaccgcgac cgcgaattgg tacccgggtc atgccacaat
      361 caaactggaa ttttcttgat caggaattgc ttacctggtg gatgacagaa gagaactttc
      421 atcaggtcat cgatcacttt ctggttatgc gtatctgtct ggagccgcaa gcttgcctac
      481 tggctgcaac ggttggcacc gcggaacaga aggcgcatct caatacgtta atggccgaaa
      541 tggtggcatt aaaagagaac tttcgccgtg aacgctggat tgaggtcgat atggcctggc
      601 atgagcatat ctatgaaatg agcgccaatc cgtttttgac ctcattcgcc tcgctattcc
      661 attcggttta tcacacttac ttcacgtcaa taaccagcga cacagtgata aagctggatc
      721 tgcaccaggc gattgtcgat gcgattatcc aaagcgatgg cgacgcggca tttaaagctt
      781 gtcaggcgct gctacgctca ccagataagt gataacccga taacaggaca ttgaatgagc
      841 gataaaaaga agcgcagtat ggcgggtttg ccgtggatcg cggcgatggc cttcttcatg
      901 caggcacttg atgccactat tctgaatacc gccttacccg caatcgctca tagccttaat
      961 cgttctcctc tcgcgatgca atcagccatc atcagttata cgctgacggt ggcgatgctt
     1021 attccggtaa gcggatggct agccgatcgc ttcggtacgc gtcgcatatt tacccttgcc
     1081 gtgagtctgt tcacgttggg ttctctggcc tgcgcacttt ctaactcgct accacagctg
     1141 gttgtcttcc gggttattca agggataggc ggcgcaatga tgatgcctgt tgctcggctg
     1201 gccttactgc gcgcttatcc tcgtaatgag cttcttcctg tattgaattt tgtcgccatg
     1261 ccgggtctgg tggggccaat tttaggcccc gttcttggcg gcgtgctggt cacctgggca
     1321 acctggcact ggatattttt aatcaatatc cccataggta ttgcgggcct tctttacgcg
     1381 cgcaaacata tgcccaattt caccaccgca cgacgcagat tcgatatcac tggctttttg
     1441 ctgtttggcc ttagccttgt tctcttctca agcggaatag agctattcgg ggaaaagatt
     1501 gtcgccagtt ggattgcctt gacggtaatt gtcaccagca tcgggttact gcttctctat
     1561 attctccatg cacgacgcac gccaaaccca ttaatttcat tagatttatt taaaacccgc
     1621 actttctcga tcggtatcgt aggcaatatt gcaacccgtc tggggaccgg ttgtgtaccg
     1681 ttccttatgc cattgatgtt acaggtagga tttggttatc aggcgtttat tgccggctgt
     1741 atgatggcac cgacagcgtt aggttccatt attgcaaaat cgatggttac ccaagtctta
     1801 cgtcgtctgg gctatcgcca tacgttagtg gggatcacgg tgattattgg gctaatgatc
     1861 gctcagttct ctttgcaatc accggcaatg gcgatatgga tgctgatctt gccgttgttt
     1921 atattaggga tggctatgtc gacgcaattt accgcgatga ataccatcac acttgccgat
     1981 ctgaccgatg acaacgccag cagcggtaac agtgttctgg cggtcacgca gcaactgtct
     2041 atcagtttag gcgttgctgt aagtgcggcc gtccttcgcg tttatgaagg gatggaaggc
     2101 acaacgactg tcgaacaatt ccactatacg tttatcacga tgggcattat tactgttgct
     2161 tcagcagcaa tgttcatgct tctgaaaaca accgatggta ataatttgat caaaagacag
     2221 cgtaaatcta agccgaaccg cgttccatca gaatcggagt aagttgtaat cgttgttgct
     2281 gaagggtcgg ttgggttatc cgatggatga gtacatcaat cgccagctcc cccagttcat
     2341 ctttcggttg atggatagtg gttaatggtg gcgtcataaa gcttgccagt tcgatatcgt
     2401 catagccaat caccgcgata tcctgcggaa cctgtaactc tgcctgatat agcgcctggt
     2461 aaacgccaac agccatagcg tcatttccgg taaagacggc ctgaggacgc agcggatgtg
     2521 ataacagttg gcgcatagcg tcaaacccgc cgttaaattc aaaatcacca gtgacttcat
     2581 agccatcagg aatgttgaga cccgcacgtt tcatcgccgc ccgataacct tccaacctca
     2641 ggcgcgccgg agttttatcc agcgggccgg taatacaggc gatacgggta tgacctttat
     2701 cgatcagata ttgcgttgct aagtctccgc ccagcaacga gttatcctga ataagatcgc
     2761 tgtcgccatc gaacggtgcc cagtccatca tcacggtagg cactgtcgga taacgttgca
     2821 tgatttcacg cgaaggctga tgcgtttcgg tgcacagtaa cagcaagcca tcaacgcgtt
     2881 tttgcatcag cgtttccaga ttgcgattca tccgttgttc atcgccttcg gtattgcaaa
     2941 ggacgagact ataaccgcgt tcgaagcagc tgcgttcaac gccacgcacc agttctgaat
     3001 agaaaggatt ggtactggca gtgatcaaca tgccaatggt atgtgtttga ttgagtttga
     3061 ggctacgcgc cagagctgat ggcgcgtaat tgagttcttt aatcgccgct tcaactttgg
     3121 cggtaatcgc ttcactgacg aagcgatctt tattgataac gtgagaaact gttgaggtag
     3181 aaacgcccgc caggcgggca acatctttca ttgtagccaa gcgtcacctc tgcctgtcta
     3241 aaaatgcgtc gatctcttca cgccacggta cggaaggttg tgcgccttta cgtgttacgg
     3301 caatcgcagc ggcagcatgg gcaaaccgaa tcgcctctgg caatggtttt tcttccagca
     3361 acgccgtgat taacgcgccg ttaaaggtat ctccggcagc aatggtatcg acagcctgca
     3421 cccggaatcc aggaacgcgc tgaccttcac cattcacgct agcccataca ccacgacttc
     3481 ctaaagtaat cagtacagta cggatacctt tttcatgcag tacctgcgcc gccttcgctg
     3541 catcttcatc attttcaaca cgaataccgg tgagcttttc tgcttccgtt tcgtttggcg
     3601 taataatgtc caccagtgcc agcagttcgt caggaagttc gcgagccgga gccgggttaa
     3661 gcgcaacgat agtcttattt tgatgggcga ttttcgccgc tgccatcaca ctttcgagtg
     3721 gtgattccag ctgcattaat aatgctgacg cgttggcaat acgctcacgt tgcgcttcca
     3781 ccagtgccgg ggaaagggca gcattagcgc cggcatgaat accgatgaca ttctcacctt
     3841 cgccattaac aaaaatcagc gccacacctg ttgattcgcc tttgatcacg ctgaccggag
     3901 aaatatcgat gttatcagtg gcgagctgct ggcgaacgct ctcaccaatg ctgtcatcgc
     3961 cagtgcaggc aataaacgcg atattcgcac cgctacgccc agcagccaca gcctgattag
     4021 cgcctttgcc gccaaatgca acctgatagt ggttaccggt tacggtttcg cctggggtag
     4081 gaaaagattg aagattaaga atgtggtcag cattaatgct gccaagaaca acgaggctgc
     4141 ctgcgttttg catattcggg atgtccataa aatgcgccac cgtgttaggg tggcgcgtgc
     4201 cctgcttttc tttatattac acgttcatct gtcgatgccg tatttatgtc accatcaggt
     4261 catacaacct gattaaaact actgcttaac aaccagtttc agatcaaccg gatacttagc
     4321 ctgaactttc tcacctttca gtactttatc ggcggtttcg acgcctttcg cgccaatctg
     4381 atcgggtagc tgagcgatag tcgctgctag tttgccatca ttcaccgctt tttcgccatc
     4441 cggtgtaccg tcaaatccga cgaccatcac atccgattta ccggcagttt gcagtgcgcg
     4501 cagcgcgccc agcgccattt catcattctg cgcgaataca gcctgaacat ccggatgtgc
     4561 ggtcaacagg ttctgcatta cgttcaaacc tttagtgcga tcaaaatctg ctggctggct
     4621 ggcaagaaca ttaaacttgt gagcagcaac agcctgctgg aagccttcgc cacgttcacg
     4681 ggctgcggat gtaccggcaa tgccttgcag ctcgataact ttggcacctt cacccgcttt
     4741 cttcgcgatg taatcaccgg cgattttgcc gcccagtacg ttatcagaag caatgtggct
     4801 caccacttca cctttcgttg cctggcggtc aagagtgata accgggatgt tcgcctggtt
     4861 agccatcttc acagcattac ccactgcgtc ggagtcggtc gggttaatca gcagaatttt
     4921 ggtgccgcga acggttaagt cctgcacgtt cgccagctct ttcgccgggt tattctggga
     4981 gtccagcacc accaggttat agccaagttt atccgcctct ttctgcgcgc catctttcag
     5041 cgatacaaag aacgggttgt taagcgtgga gaccaccagc gcgatggtgt cttttgccat
     5101 cgcattcgca ctgacggtgg cgcttagcgc aacagcggaa accagggtag ccagtttttt
     5161 catgttcata ttcaagatgt cctgtagtcg ttattactgc tttttgttgt ctaccagcac
     5221 cgccagcaaa atcaccaccg ctttgacgat catctggtaa taggaggaaa cacctaacaa
     5281 attcaatcca ttattaagga agccaagaat taatgcgccg atcaacgtcc caacaatgcg
     5341 accttttccg cccgccagac tcgtaccgcc cagaaccacc gcagcaatgg catccagctc
     5401 atagccagtc cccgccgtag gttgtgcgga ggagagacgc gccacttcaa tgatcccggc
     5461 cagcgatgcc agcagaccac aaagagaata gacgatgatt ttgattttat tgacgttgat
     5521 accagaaagg cgcgttgccg cttcgttgcc gcccagcgcg tagatgtaac gccccagacg
     5581 cgtgtgatgc agcatgtacc aggccgcgag gaagacaatt cccatgatcc agactggcgt
     5641 cggtacaccc agcggacgac caataccaaa ccagccaaac agatcggcgt tctcagtaaa
     5701 gccagtattc actgggctac cgttggtata aaccatggtc acgccgcgca gtaaaagcat
     5761 cataaccagc gtagcgataa aggcctggac gcgacctttc gctacaatca ccccggttac
     5821 cgcaccaatt gcggcaccta acgcgagagc ggcagcgaca gccaccagcg cattgacttc
     5881 aatgccaacg atagatgcag caactgcgcc ggtcagcgcc aatagagaac ctaccgacaa
     5941 gtcgatgccc gacgtcagga tcaccagcgt catcccgact gccataatgg cgttcaccga
     6001 ggtttgctgg agaatattga ataagttatt gatggtgaaa aagttcgggc ttaacgtcga
     6061 gacaatcgcg atcagcacca gcagagcgat aagcgatttc tgctccatca gccacgcttt
     6121 cgtgaaataa cggcgaccag agacagtctg ggttgtcatt tttttactcc tgattcacgc
     6181 gattaagctt gcccacagcg gcagccatta acacttcctg ggtggcctgc tcacgagtaa
     6241 attccccgct gagatgccct tcatgcatga cgatgatgcg atcgctcatg cctaatactt
     6301 ctggcatctc cgatgacacc agaatgatgc tcaagccatc ggctttgaac tggttaatca
     6361 gttgatagat ctcttttttc gcgccgacat ctacgccacg ggtaggctca tcgaggatca
     6421 acactttggg gcgtgtcatc agaccacggg caatcgccac tttttgctga ttgccaccgg
     6481 agagcagacc aattgcctgt tccatcgacg gcgttttcac attaaacaga cgaatgaaat
     6541 cacttactgc ctgctgttca tcggcatgct tcaaactgcc gccagcgcgg ctgaagtagc
     6601 gcagcgctgt cagcgacatg ttctctttta ctgacatgcc cagcactaaa ccgtcacgtt
     6661 tacggtcttc ggagatatac acaatgccgt ttgccaggcc atcctgcggt gaacgggtaa
     6721 cgacttcatg cccatccagg gtgacgtaac cgctggtgcg cggcagtgcg ccgtagagca
     6781 ctttcatcag ttcggtacga cctgcgccca tcaaaccaga gacgccaaga atttcgcctt
     6841 tgcgtaaagt aaaagagaca tcgttaacgc caggtccgca gagattatcg actttcaggc
     6901 ggatatctcc cggcgctttg tccaggtgcg gatattgatc ttccagcttg cgacccacca
     6961 tcatctcaat cagcgaatct tcggtcagtg atgctacttc acgctcagca ataaattgcc
     7021 catcacgaaa aacggtaacg tcatcgcaaa tctcgaagat ttctttcatg cggtgggaga
     7081 tatagacaat accgcggcct tgcgatttca gctcgcggat gacgcggaac aaggattcgg
     7141 tttcggtatc ggtcagcgca tcggtcggtt catccataat gatgactttc gactcaaagc
     7201 tcagcacttt ggcaatttca accatttgct ggtcaccgat ggaaagatcg cccaccagct
     7261 tgtcgctttt aaagcgcagg ttaagtttag ccagcaattt atccgcttcg gcatacatgg
     7321 ttttccagtc aattttgcca aagcgattaa caaactcacg accgaggaaa atgttttcgg
     7381 caatggttaa ctgcgggatc aggttcagtt cctggtggat aatcccaatc ccggcttcct
     7441 gggaagattt cggcccggta aatgtcgttt ctttccccag ccataaaagc gtgccggcat
     7501 cgcgagtata gataccagta agcactttca tcatggtgga tttacccgcg ccgttttcgc
     7561 ccaccagcgc catcacgcgg cccggataga catttaacgc tgcgcccgag agggctttta
     7621 cgcccgggaa ggctttatcg atgcctttaa gctgaagtaa tgcttccatg acggcctcag
     7681 aacgtcacgc cagcacagag aatgatattc gcatacggag aacattctcc gctgcgaatt
     7741 accgcctgac tttctgcggt ttgttgtttg aattgttcat gcgtggtgta acgaatttca
     7801 atggtatttc cctggtgttt ttgcagctgc tcaaggtgag tgagcaacgt ttcgtggagt
     7861 tgcggattat gttgtttgat ctcttccgcg ataatggcag cttcgacctg catttcattt
     7921 gtgacgacgc ccagcacctg cataaaagaa ggtaccccct gggttaatgc catatcgata
     7981 cgcgttgtac ttttggggat gggtaaacca gcatcacaca ccaccagcgt atcggtatgt
     8041 cccagacggg agatcaccga tgaaatatca gaattaagaa cggtgccttt tttcattttt
     8101 ttctccatca gcgaaacgtt tcgctgaccc gcagtttaat cctgactgag ttgggaaaac
     8161 catcgtgacg ttacggaaat gtgatcaacc tcgaaacgtt ttacatggtg attaaccatg
     8221 aaaacaaaaa cgccccctta tggagaaagg aggcgtctgg cgttagattt cgacctgagt
     8281 acccagttcg ataaccctgt ttggcgggat ttcaaattga tccggcgcac gcagcgcatt
     8341 acgttgcagc agcaagtaca gcttgccgcg cagacgcaaa taccacgggc gtttgccgag
     8401 gatcaacgac tcatgcgaca taaagaagga ggtttccatc atccggcaac ttaatccttc
     8461 cagaccgcag cggtggaaaa cttcttccac gtttggcgtt tctcgccaac cataacttgc
     8521 caccacgcgc cagaaagtgg gcgacagttg ttcaatctgt acccgacgga cgttatggac
     8581 atatggcgcg tcttcggtgc gcagagttaa cagaatcacc cgctcatgca ataccttgtt
     8641 atgtttcagg ttatgcatca gcgcaaaggg aatgacgttg attgcacgcg acatatacac
     8701 cgcggtccca ggcacgcgaa cgggcggtga tttctccagc gaagcaatca ttgcttccag
     8761 agagttacca tgttcatgca tccgccgcag caagcggaaa cgctcgctct tccaggtggt
     8821 catcacgata aacatcaccg taccgaggct taatggcaac cagccgccgg agagcagttt
     8881 atcgaggtta gcggtgaaca agggaatatc gacacaaagg aaagcaatca ggatcagcgc
     8941 aacaaaatac ttattccagt gccagttctg acgtgccacg gtagtcgaga gaatagacgt
     9001 cagcaccatg gttccggtca ccgcaatccc gtacgccgcc gccaggttgc tggagtgctc
     9061 aaagctgaca atcacaatca cgaccgcgac atagagcatc cagttcacaa agggaatata
     9121 gatttgccct gactccattt cggaggtgtg aataatgcgc atcggcgaca aatatcccag
     9181 acgtaccgcc tgacgcgtca atgagaagac gccagagata accgcctgcg aggcaattac
     9241 cgtcgccagt gcggcgatga tcagcagcgg gatcagcgcc cagtccggtg ccaacaggaa
     9301 gaacgggttc ttaatcgctt ccggattctt taacaacagc gctccctggc cgaagtaatt
     9361 aagggttaag gaaggcaata cgacggtgaa ccacgccaga cgaataggga acttaccaaa
     9421 gtgccccata tcagcataca gcgcctcgac ccccgtaatc gacagcacca ctgcccctaa
     9481 tgcaataaaa gaaaccgttt tgtattcaag gaagaaatgc accgcccaca tcggattcag
     9541 tgcatgcagc acttccgggt tagcaataat gctacgtaac cccagccctg ccagaatcaa
     9601 aaaccaggtc agcatgatcg gtgcaaacag cttaccgacc atagccgtgc catgtttttg
     9661 aatcataaat aataaagtga gaacgataat tgagagggga actatccagg tatccagctg
     9721 cggggcgacg atttccagac cttcaatggc tgacatcacc gaaatagcgg gtgttatgac
     9781 gacttcacca tagaaaaagc tgccgccgat tagccccata atcaccagca ttgatgtggt
     9841 tcgcgccgac gtattacgcc cggcaagcga catcaacgtc aggatccccc cttcaccggc
     9901 gttatctgcc cgcatcacga aggtgagata tttaatggaa accacaaaga ttagcagcca
     9961 gaagatcagc gataaaaagc caaacacggc atcgcgttca acgccaaaac caaactggcc
    10021 ggacaaacat tcacgaagtg tatataacgg gctggtacca atatcgccgt agacaactcc
    10081 aatcgccgcg agggtaatcg cgggcaatga ttgcttatta tcagtgctca tagactagtc
    10141 tttcgttgaa atatgaaatg tgtgcttagt cccttggccc acaaaaaagc gcacagtatg
    10201 cacgattaac ggcaaaatcg tactcctaaa tgcggccaca ttaacctggc tcaaagaaaa
    10261 ataccccccc tttgagacta ttttagccac cagcaagaaa cgtctatact cgcaatttac
    10321 gcagaacttt tgacgaaagg acgccacttc attatggctc accctcattt attagcggaa
    10381 agaatttccc gcctgagcag ttcgttggaa aaggggcttt atgaacgtag ccacgccatc
    10441 cgcttgtgtt tattagcggc attaagtggt gaaagtgtgt tcctccttgg cccgccaggt
    10501 attgccaaaa gtttgatcgc ccggcgctta aaattcgcct ttcagaatgc ccgcgcgttt
    10561 gaatatctga tgactcgctt ctccacgccg gaagaggttt ttggtcccct ttctattcag
    10621 gcgctaaaag atgaagggcg ctatgaacgt ttaaccagcg gttacctgcc ggaagcagaa
    10681 atcgtctttc tggatgagat ctggaaagcg ggtccggcaa ttcttaatac cctgctcacc
    10741 gccattaacg agcgccagtt ccgcaacggt gcacacatag aaaaaatccc gatgcgcctg
    10801 ctggtggcgg cctccaacga gctgccggaa gcagacagca gtctggaagc gttatatgac
    10861 cgcatgctga ttcgtctgtg gttagataaa gtgcaggata aagcgaattt ccgctccatg
    10921 ctgaccagtc aacaggatga aaacgacaat ccggttccta cctccttgca ggtcacagat
    10981 gaagaatatg aacgctggca gaaagagatt ggtgaaatta cgctgcccga tcatgtattt
    11041 gagctgattt ttatgctgcg ccagcaactg gataaattac cggatgcgcc ttatgtctcg
    11101 gatcgtcgct ggaaaaaagc gattcgatta ttgcaggcca gcgccttttt tagcggtcgc
    11161 agtgctgttg ccccggttga tctcattttg ctgaaagatt gcctgtggta tgacgcgcaa
    11221 agcctgaatt tgatacaaca acaaatcgat gtattgatga ccggtcatgc ctggcaacag
    11281 caagggatgt tgacccgcct gggcgcgatt gtgcaacgtc acctgcaact acagcagcaa
    11341 caaagcgata aaacagcctt aacggtaatt cgtctgggcg gcattttcag ccgtcgtcag
    11401 cagtatcaac tccctgttaa cgttactgct tccactctga ctctgctgct gcaaaaaccg
    11461 ttaaaactgc atgatatgga agtggttcat atcacctttg agcgtagcgc gctggaacag
    11521 tggctgagca aaggtggtga aattcgcggc aaactaaacg gtatcggctt tgcccagaaa
    11581 ctgaatctgg aagttgatag cacccaacat cttgttgtac gcgatgtaag tttacaaggc
    11641 agtacgctgg cacttcccgg ttcatcggct gaaggtctgc caggtgaaat aaaacaacaa
    11701 ctggaagagc ttgaaagcga ctggcgcaag caacacgctt tattcagcga acagcaaaaa
    11761 tgtctgttta tccctggcga ctggttaggt cgcattgaag ccagcctaca ggatgtcggt
    11821 gcacagattc gccaggcgca acaatgctaa cgctggatac gcttaatgtg atgctggccg
    11881 tcagcgaaga gggattgatc gaagagatga tcatcgcgct gctggcctca ccgcagctgg
    11941 cggtcttctt tgaaaaattc ccacgactga aggcggctat cactgatgat gttccccgct
    12001 ggcgtgaggc gctgcgcagt cggctgaaag atgcccgagt cccgccggaa ctcaccgaag
    12061 aggtgatgtg ctatcagcaa agccagctcc tctccacgcc gcagtttatt gtgcagctac
    12121 cacagatcct ggacttactg catcgtctga attccccatg ggcagaacaa gcccgacagt
    12181 tggttgatgc taacagcacg atcacttcag cgttacacac gctttttctc cagcgttggc
    12241 gtttaagtct gatcgtgcaa gcaacgacgt taaatcaaca gctattagaa gaagaacgcg
    12301 aacaactgtt gagtgaagtt caggaacgca tgacgcagag cggacaactt gaaccgattc
    12361 tcgcagataa caataccgca gctggtcgtc tgtgggatat gagcgccggt cagcttaaac
    12421 gtggcgacta tcagttgatt gtgaaatacg gtgaatttct gaacgaacag ccggaactga
    12481 aacgcctggc agaacagttg gggcgttccc gggaagccaa atcaataccg cgcaacgatg
    12541 cgcagatgga aaccttccgc accctggtgc gcgaaccggc gacggttcct gagcaggttg
    12601 atggtctgca acaaagcgat gatattttac gtctcctgcc gccagaactg gcgacactag
    12661 ggataacaga actggagtat gagttttacc gtcggctggt ggaaaaacag ttgctcacct
    12721 atcgcctgca cggtgagtcg tggcgtgaaa aagtgatcga acgcccggtg gtgcataaag
    12781 attacgacga acagccgcgc ggaccgttta ttgtctgtgt ggatacttcc ggctcaatgg
    12841 gcggctttaa tgaacagtgt gcgaaagcgt tctgcctggc cttgatgcgc attgctctcg
    12901 ctgaaaaccg gcgctgctat attatgctat tttccaccga gatcgtccgt tatgagcttt
    12961 caggcccaca aggcatcgaa caggcaatcc gttttttaag ccagcgtttt cgtggtggta
    13021 ctgatcttgc cagttgtttt cgcgccatta tggaacgatt gcaaagccgg gaatggtttg
    13081 acgccgatgc ggtggtgatt tctgatttta tcgcccagcg gttgcctgac gacgtgacga
    13141 gtaaagtgaa agagttgcag cgggtacatc agcatcgctt tcatgccgtg gcgatgtcgg
    13201 cacatggcaa acccggcatc atgcgcattt tcgatcatat ctggcgcttt gataccggga
    13261 tgcgaagccg cctgctcaga cgctggcgac gataaacgat tacagcagag aagggacgct
    13321 ctcacgaact gccgctgacc atactccaca ctgaacctgg ccgatatgcg gcagttgcag
    13381 cagcagcatg gtcaaacgag actggccgat accaccgccg atggtctgcg gcatttcacc
    13441 gcgcagcagc gcctgatgcc actccagttg caggcgatct tcgtcaccgg tcagcgccag
    13501 ctgatgcttc agcgtgtcgg catcaacgcg gatccccatg gaagaaagct caaacgcatc
    13561 ttccagtacc gggttccaca ccagaatatc gccgttcagc cccgcatagc ccagctctga
    13621 cggggtgctc cagtcatcat aatccggtgc gcgcacgtcg tgacgatggc catcactcaa
    13681 cttgccgcca atcccgacga gaaataccgc gccaagatct ttcgcaatcg cccgctcacg
    13741 ccctttggca tcaagattcg gataacgaga cagtaactcc tggctgtgta cgaagtggat
    13801 ctgatccggc aggaacggtg ccaggccaaa ctcttcgcta accgcagctt cggttgcttt
    13861 aattcccgcc cagatcgcct ctaccgtgct tttcagagtc gagaattgac gctcgccgtc
    13921 ggccattacg cgttcccagt cccactggtc aacatagacc gagtgcaacg gagaaagacg
    13981 gtcttcatcg gggcgaaggg ctttcatgtg cgtgtaaagc ccttcgcccg cactgaagtc
    14041 gtgttgccct aatgtctgac gtttccactt cgccagtgaa tgaaccactt cgaactgggc
    14101 atcaggcaga gctttcactt ttacctgcac cgctttttca cagcccgaca agttatcctg
    14161 cgtgccatcc cccacacggc taagaatcgg cgcctggact tcaatcagcc ctaagcgttc
    14221 ttccagttga cgggaaaagt gagatttcac gaagctaatt tgacgttgtt tggcaatgta
    14281 agcggttttc attttttata ctcctgcgtc ctgttgatta agattaagca acaaaaattg
    14341 gccctcatgc aatattcatt caataaaaag ccatagtgac ttttgattca ttaaaaaata
    14401 aggctaaaat agagtgaatc attaattctc ataagaaaaa cctatggaaa attatctgat
    14461 cgacaatctg gaccgcggca tcctggaagc attaatggaa aatgcacgca ctgcttacgc
    14521 cgaactggcg aaacaatttg gtgtaagccc ggggacgatt cacgttcgtg tagagaaaat
    14581 gaagcaggcg ggaatcatta caggggcgcg tattgacgtc agcccgaaac agctcggtta
    14641 tgacgtaggc tgctttatcg gcattatatt gaagagtgcc aaagactatc cttccgcgct
    14701 ggcaaagctg gaaagccttg atgaagtcac cgaggcctac tacaccaccg gccactacag
    14761 catctttatc aaagtgatgt gccgttcgat cgacgccctc cagcatgtac ttatcaacaa
    14821 gatccaaaca attgatgaaa ttcagtccac cgagacactg atcgtcctgc aaaacccgat
    14881 catgcgtacc atcaagccat gatcggcttt tttaatccca tacttttcca caggtagatc
    14941 ccagcgcgtt cacagcgtac aatacgccac tcttaataaa ggtggcggtt tatggcagat
    15001 atcactctta tcagcggcag caccctcggc ggtgccgaat atgtagcaga acacctggct
    15061 gaaaagctgg aagaggcggg ttttaccacc gaaacgctgc acggtccgct gttggaagaa
    15121 ttatctgcct cggggatctg gctggttatc agttccaccc acggtgccgg agatattccg
    15181 gacaaccttt ctcctttcta tgaagcatta caggaacaaa agcccgatct ttctgcggta
    15241 cgctttggcg caatcggtat tggcagtcgt gaatacgaca ccttttgtgg ggctatcgat
    15301 aaactcgagg cagaactcaa aaattccggt gcaaaacaga caggcgaaac actgaagatc
    15361 aacatccttg atcacgacat tcctgaagat ccggcagaag aatggctggg atcgtggatt
    15421 aatttactca aataagtata cagatcgtgc gatccactgt ggataactct gtcaggaagc
    15481 ttggatcaac cggtagttat ccaaagaaca acggttgttc agtttttgag ttgtgtataa
    15541 cccctcattc tgatcccagc ttatacggtc caagatcacc gatcattcac agttaatgat
    15601 cctttccagg ttgttgatct taaaagccgg atccttgtta tccacagggc agtgcgatcc
    15661 taataagaga tcacaataga acagatctct aaataaatag atcttctttt taatacccag
    15721 gatcccaggt ctttctcaag ccgacaaagt tgagtagaat ccacggcccg ggcttcaatc
    15781 cattttcata ccgcgttatg cgaggcaatc accatgtttt atccggatcc ttttgacgtc
    15841 atcatcattg gcgggggtca tgcaggcacc gaggccgcga tggccgcggc gcgtatgggt
    15901 caacagactc tgcttttgac acacaatatc gacactctgg ggcagatgag ctgcaacccg
    15961 gcgatcggcg gtattgggaa gggacatctg gtaaaagaag tggatgcact cggcggtctg
    16021 atggcgaaag cgatcgatct ggcgggtatc cagtttagga tactaaacgc aagtaaggga
    16081 ccggcagttc gcgctacccg agctcaggcg gatcgtgtgc tctaccggca ggcggtacgt
    16141 acggcgctgg agaaccaacc gaacctgatg atcttccagc aggcagttga agatcttatt
    16201 gtcgaaaacg atcgcgtggt cggagccgtt acccaaatgg gactgaagtt ccgtgccaaa
    16261 gctgtcgtgc tcaccgttgg gacgttcctc gacggtaaaa ttcatatcgg tctggataac
    16321 tacagcggtg gccgtgctgg tgatccgccg tccattccgc tttctcgccg tttgcgtgaa
    16381 ctgccgctgc gcgttggtcg tctgaaaacc gggacaccac cgcgtattga tgctcgaacc
    16441 atcgacttta gcgtactggc gcaacagcat ggcgataacc caatgccggt attctcgttt
    16501 atgggcaatg cgtcccagca tccccagcag gtgccgtgtt atatcactca taccaacgag
    16561 aaaacccatg atgtgatccg cagtaacctc gatcgtagcc caatgtacgc aggggtgatc
    16621 gaaggtgtcg gcccacgcta ctgcccgtcg atcgaagaca aagtcatgcg cttcgccgac
    16681 agaaatcagc atcagatctt ccttgaaccg gaagggctga cctctaacga aatttatccg
    16741 aacggtatct ccaccagcct gccgttcgat gtgcagatgc aaatcgtccg ctccatgcag
    16801 gggatggaaa acgcgaagat cgtgcgtccg ggttatgcca ttgagtatga cttcttcgat
    16861 ccacgcgacc tgaaaccgac gctggagagc aagtttatcc aggggctgtt ctttgctggt
    16921 cagattaacg gcactaccgg ttacgaagaa gccgctgcgc aaggtttgct ggctggtctt
    16981 aacgctgccc gtctgtctgc agacaaagaa ggttgggctc cggcgcgttc tcaggcgtat
    17041 ctcggcgtac tggttgatga cctgtgcact ttaggaacca aagaaccgta tcgtatgttt
    17101 acctcgcgcg cagaatatcg tctgatgctg cgcgaagata atgcggatct gcgtttgact
    17161 gaaatcggtc gtgaactggg cctggtggat gacgaacgtt gggcgcgctt taacgagaaa
    17221 cttgagaata tcgagcgtga gcgtcagcgt ctgaaatcga cctgggtaac cccgtcggcg
    17281 gaagctgcag ccgaagtgaa tgctcacctg actgcgccac tttcccgtga agccagtggt
    17341 gaagatctgc tgcgtcgtcc ggaaatgact tatgaaaaat taaccacgct gacgccgttt
    17401 gcccctgcgt tgacagacga acaggcggcg gaacaggttg agattcaggt taaatacgaa
    17461 ggttatatcg cgcgccagca agatgagatc gaaaagcagc tgcgtaacga gaacaccctg
    17521 ctaccagcga cgctggatta ccgccaggta tccggtcttt ctaacgaagt gatcgccaaa
    17581 cttaacgatc acaaaccggc ctctatcggt caggcttcgc gtatttctgg cgtcacgcct
    17641 gcggccatct ccattctgct ggtgtggctg aaaaaacagg gtatgctgcg tcgtagcgca
    17701 taacgcatta aaaatgcctg gtaagcaccc gcttaccagg caacgcatca agaacaggta
    17761 atcaccgtgc tcaacaaact ctccttactg ctgaaagacg caggtatttc gcttaccgat
    17821 caccagaaaa accagcttat tgcctacgtg aatatgctgc ataaatggaa taaagcgtac
    17881 aacctgactt cggttcgcga tcctaatgag atgctggtac gccatattct cgatagcatt
    17941 gtggtagctc cgtatctgca aggtgaacgg tttatcgatg tcggcaccgg accaggactg
    18001 cctggcattc cactctctat cgtgcgtcct gaagcccact tcacgttgtt ggatagcctt
    18061 ggtaaacgcg tgcgtttcct tcgtcaggtg caacatgagc ttaaactgga gaatattgag
    18121 ccagtacaga gcagggtaga agagtttcct tctgagccgc catttgatgg cgtaattagc
    18181 cgcgcttttg cctctctgaa cgatatggtg agctggtgcc accatcttcc tggtgagcaa
    18241 ggtcgtttct acgcgctgaa aggccaaatg ccggaagatg aaatcgcttt gttgcccgaa
    18301 gaatatcagg tcgaatcagt ggttaaactt cacgttccgg ccctggatgg cgaacgtcat
    18361 ctggtgatga ttaaagcaaa taaaatttaa tttttatcaa aaaaatcata aaaaattgac
    18421 cggttagact gttaacaaca acccggtttt ctactgatat aactggttac atttaacgcc
    18481 acgttcactc ttttgcatca acaaagtaac gtggcttttt ttggtaagca gaaaataagt
    18541 cataagtgaa aatatcagtc tgctaaaaat cggcgctaag aatcatcatt ggctgttaaa
    18601 acattattaa aaatgtcaat gggtggtttt tgttgtgtaa atgtcattta ttaaaacagt
    18661 atctgttttt agactgaaat atcataaact tgcaaaggca tcatttgcca agtaaataaa
    18721 tatgctgtgc gcgaacatgc tcaatatgtg atctgaagca cgctttatca ccagtgttta
    18781 cgcgttattt acagtttttc atgatcgaac agggtttgca gaaaagtcgc aattgtatgc
    18841 actggaaaaa tatttaaaca tttattcacc ttttggctac ttattgtttg aaatcacggg
    18901 ggcgcaccgt ataatttgac cgctttttga tgcttgactc taagccttaa agaaagtttt
    18961 atacgacacg cggcatacct cgaagggagc aggagtgaaa aacgtgatgt ctgtgtcgct
    19021 cgtgagtcga aacgttgctc ggaagcttct gctcgttcag ttactggtgg tgatagcaag
    19081 tggattgctg ttcagcctca aagacccctt ctggggcgtc tctgcaataa gcgggggcct
    19141 ggcagtcttt ctgcctaacg ttttgtttat gatatttgcc tggcgtcacc aggcgcatac
    19201 accagcgaaa ggccgggtgg cctggacatt cgcatttggc gaagctttca aagttctggc
    19261 gatgttggtg ttactggtgg tggcgttggc ggttttaaag gcggtattct tgccgctgat
    19321 cgttacgtgg gttttggtgc tggtggttca gatactggca ccggctgtaa ttaacaacaa
    19381 agggtaaaag gcatcatggc ttcagaaaat atgacgccgc aggattacat aggacaccac
    19441 ctgaacaacc ttcagctgga cctgcgtaca ttctcgctgg tggatccaca taacccccca
    19501 gccaccttct ggacaatcaa tattgactcc atgttcttct cggtggtgct gggtctgttg
    19561 ttcctggttt tattccgtag cgtagccaaa aaggcgacca gcggtgtgcc aggtaagttt
    19621 cagaccgcga ttgagctggt gatcggcttt gttaatggta gcgtgaaaga catgtaccat
    19681 ggcaaaagca agctgattgc tccgctggcc ctgacgatct tcgtctgggt attcctgatg
    19741 aacctgatgg atttactgcc tatcgacctg ctgccgtaca ttgctgaaca tgtactgggg
    19801 ctgccggcac tgcgtgtggt tccgtctgcg gacgtgaacg taacgctgtc tatggcactg
    19861 ggcgtattta tcctgattct gttctacagc atcaaaatga aaggcatcgg cggcttcacg
    19921 aaagagttga cgctgcagcc gttcaatcac tgggcgttca ttcctgtcaa cttaatcctt
    19981 gaaggggtaa gcctgctgtc caaaccagtt tcactcggtt tgcgactgtt cggtaacatg
    20041 tatgccggtg agctgatttt cattctgatt gctggtctgt tgccgtggtg gtcacagtgg
    20101 attctgaatg tgccgtgggc cattttccac atcctgatca ttacgctgca agccttcatc
    20161 ttcatggttc tgacgatcgt ctatctgtcg atggcgtctg aagaacatta atttaccaac
    20221 actactacgt tttaactgaa acaaactgga gactgtcatg gaaaacctga atatggatct
    20281 gctgtacatg gctgccgctg tgatgatggg tctggcggca atcggtgctg cgatcggtat
    20341 cggcatcctc gggggtaaat tcctggaagg cgcagcgcgt caacctgatc tgattcctct
    20401 gctgcgtact cagttcttta tcgttatggg tctggtggat gctatcccga tgatcgctgt
    20461 aggtctgggt ctgtacgtga tgttcgctgt cgcgtagtaa gcgttgcttt tatttaaaga
    20521 gcaatatcag aacgttaact aaatagaggc attgtgctgt gaatcttaac gcaacaatcc
    20581 tcggccaggc catcgcgttt gtcctgttcg ttctgttctg catgaagtac gtatggccgc
    20641 cattaatggc agccatcgaa aaacgtcaaa aagaaattgc tgacggcctt gcttccgcag
    20701 aacgagcaca taaggacctt gaccttgcaa aggccagcgc gaccgaccag ctgaaaaaag
    20761 cgaaagcgga agcccaggta atcatcgagc aggcgaacaa acgccgctcg cagattctgg
    20821 acgaagctaa agctgaagca gagcaggaac gtactaaaat cgtggcccag gcgcaggcgg
    20881 aaattgaagc cgagcgtaaa cgtgcccgtg aagagctgcg taagcaagtt gctatcctgg
    20941 ctgttgctgg cgccgagaag atcatcgaac gttccgtgga tgaagctgct aacagcgaca
    21001 tcgtggataa acttgtcgct gaactgtaag gagggagggg ctgatgtctg aatttattac
    21061 ggtagctcgc ccctacgcca aagcagcttt tgactttgcc gtcgaacacc aaagtgtaga
    21121 acgctggcag gacatgctgg cgtttgccgc cgaggtaacc aaaaacgaac aaatggcaga
    21181 gcttctctct ggcgcgcttg cgccagaaac gctcgccgag tcgtttatcg cagtttgtgg
    21241 tgagcaactg gacgaaaacg gtcagaacct gattcgggta atggctgaaa atggtcgtct
    21301 taacgcgctc ccggatgttc tggagcagtt tattcacctg cgtgccgtga gtgaggctac
    21361 cgctgaggta gacgtcattt ccgctgccgc gttgagtgaa caacagctcg cgaaaatttc
    21421 tgctgcgatg gaaaaacgtc tgtcacgcaa agttaagctg aattgcaaaa tcgataagtc
    21481 tgtaatggca ggcgttatca tccgagcggg tgatatggtc attgatggca gcgtacgcgg
    21541 tcgtcttgag cgccttgcag acgtcttgca gtcttaaggg gactggagca tgcaactgaa
    21601 ttccaccgaa atcagcgaac tgatcaagca gcgcattgct cagttcaatg ttgtgagtga
    21661 agctcacaac gaaggtacta ttgtttctgt aagtgacggt gttatccgca ttcacggcct
    21721 ggccgattgt atgcagggtg aaatgatctc cctgccgggt aaccgttacg ctatcgcact
    21781 gaacctcgag cgcgactctg taggtgcggt tgttatgggt ccgtacgctg accttgccga
    21841 aggcatgaaa gttaagtgta ctggccgtat cctggaagtt ccggttggcc gtggcctgct
    21901 gggccgtgtg gttaacactc tgggtgcacc aatcgatggt aaaggtccgc tggatcacga
    21961 cggcttctct gctgtagaag caatcgctcc gggcgttatc gaacgtcagt ccgtagatca
    22021 gccggtacag accggttata aagccgttga ctccatgatc ccaatcggtc gtggtcagcg
    22081 tgaattgatc atcggtgacc gtcagaccgg taaaaccgca ctggctatcg atgccatcat
    22141 caaccagcgc gattccggta tcaaatgtat ctatgtcgct atcggccaga aagcgtccac
    22201 catttctaac gtggtacgta aactggaaga gcacggcgca ctggctaaca ccatcgttgt
    22261 ggtagcaacc gcgtctgaat ccgctgcact gcaatacctg gcaccgtacg ctggttgcgc
    22321 aatgggcgaa tacttccgtg accgcggtga agatgcgctg atcatttacg atgacctgtc
    22381 taaacaggct gttgcttacc gtcagatctc cctgctgctc cgtcgtccgc caggacgtga
    22441 agcattccct ggcgacgtat tctacctcca ctctcgtctg ctggagcgtg ctgcgcgtgt
    22501 taacgccgaa tacgttgaag ccttcaccaa aggtgaagtg aaagggaaaa ccggttctct
    22561 gactgcgctg ccgattatcg aaactcaggc gggtgacgtt tctgcgttcg ttccgaccaa
    22621 cgtaatctcc attaccgatg gtcagatctt cctggaaacc aacctgttca acgccggtat
    22681 tcgtcctgcg gttaacccgg gtatttccgt atcccgtgtt ggtggtgcag cacagaccaa
    22741 gatcatgaaa aaactgtccg gtggtatccg taccgctctg gcacagtatc gtgaactggc
    22801 agcgttctct cagtttgcat ccgaccttga cgatgcaaca cgtaagcagc ttgaccacgg
    22861 tcagaaagtg accgaactgc tgaaacagaa acagtatgcg ccgatgtccg tagcgcagca
    22921 gtctctggtt ctgttcgcag cagaacgtgg ttacctggcg gatgttgaac tgtcgaaaat
    22981 cggcagcttc gaagccgctc tgctggctta cgtcgaccgt gatcacgctc cgttgatgca
    23041 agagatcaac cagaccggtg gctacaacga cgaaatcgaa ggcaagctga aaggcatcct
    23101 cgattccttc aaagcaaccc aatcctggta acgtctggcg gcttgcctta gggcaggccg
    23161 caaggcattg aggagaagct catggccggc gcaaaagaga tacgtagtaa gatcgcaagc
    23221 gtccagaaca cgcaaaagat cactaaagcg atggagatgg tcgccgcttc caaaatgcgt
    23281 aaatcgcagg atcgcatggc ggccagccgt ccttatgcag aaaccatgcg caaagtgatt
    23341 ggtcaccttg cacacggtaa tctggaatat aagcaccctt acctggaaga ccgcgacgtt
    23401 aaacgcgtgg gctacctggt ggtgtcgacc gaccgtggtt tgtgcggtgg tttgaacatt
    23461 aacctgttca aaaaactgct ggcggaaatg aagacctgga ccgacaaagg cgttcaatgc
    23521 gacctcgcaa tgatcggctc gaaaggcgtg tcgttcttca actccgtggg cggcaatgtt
    23581 gttgcccagg tcaccggcat gggggataac ccttccctgt ccgaactgat cggtccggta
    23641 aaagtgatgt tgcaggccta cgacgaaggc cgtctggaca agctttacat tgtcagcaac
    23701 aaatttatta acaccatgtc tcaggttccg accatcagcc agctgctgcc gttaccggca
    23761 tcagatgatg atgatctgaa acataaatcc tgggattacc tgtacgaacc cgatccgaag
    23821 gcgttgctgg ataccctgct gcgtcgttat gtcgaatctc aggtttatca gggcgtggtt
    23881 gaaaacctgg ccagcgagca ggccgcccgt atggtggcga tgaaagccgc gaccgacaat
    23941 ggcggcagcc tgattaaaga gctgcagttg gtatacaaca aagctcgtca ggccagcatt
    24001 actcaggaac tcaccgagat cgtctcgggg gccgccgcgg tttaaacagg ttatttcgta
    24061 gaggatttaa gatggctact ggaaagattg tccaggtaat cggcgccgta gttgacgtcg
    24121 aattccctca ggatgccgta ccgcgcgtgt acgatgctct tgaggtgcaa aatggtaatg
    24181 agcgtctggt gctggaagtt cagcagcagc tcggcggcgg tatcgtgcgt accatcgcaa
    24241 tgggttcctc cgacggtctg cgtcgcggtc tggatgtaaa agacctcgaa cacccgatcg
    24301 aagtcccggt aggtaaagcg actctgggcc gtatcatgaa cgtactgggt gaaccggtcg
    24361 acatgaaagg cgagatcggt gaagaagagc gttgggcgat tcaccgcgca gcaccttcct
    24421 acgaagagct gtcaaactct caggaactgc tggaaaccgg tatcaaagtt atcgacctga
    24481 tgtgtccgtt cgctaagggc ggtaaagttg gtctgttcgg tggtgcgggt gtaggtaaaa
    24541 ccgtaaacat gatggagctt attcgtaaca tcgcgatcga gcactccgga tactctgtgt
    24601 ttgcgggcgt aggtgaacgt actcgtgagg gtaacgactt ctaccacgaa atgaccgact
    24661 ccaacgttat cgacaaagta tccctggtgt atggccagat gaacgagccg ccgggaaacc
    24721 gtctgcgcgt tgctctgacc ggtctgacca tggctgagaa attccgtgac gaaggtcgtg
    24781 acgttctgct gttcgttgac aacatctatc gttacaccct ggccggtacg gaagtatccg
    24841 cactgctggg ccgtatgcct tcagcggtag gttatcagcc gaccctggcg gaagagatgg
    24901 gcgttctgca ggaacgtatc acctccacca aaactggttc tatcacctcc gtacaggcgg
    24961 tatacgtacc tgcggatgac ttgactgacc cgtctccggc aaccaccttt gcgcaccttg
    25021 acgcaaccgt ggtactgagc cgtcagatcg cgtctctggg tatctacccg gccgttgacc
    25081 cgctggactc caccagccgt cagctggacc cgctggtggt tggtcaggaa cactacgaca
    25141 ccgcgcgtgg cgttcagtcc atcctgcaac gttatcagga actgaaagac atcatcgcca
    25201 tcctgggtat ggatgaactg tctgaagaag acaaactggt ggtagcgcgt gctcgtaaga
    25261 tccagcgctt cctgtcccag ccgttcttcg tggcagaagt attcaccggt tctccgggta
    25321 aatacgtctc cctgaaagac accatccgtg gctttaaagg catcatggaa ggcgaatacg
    25381 atcacctgcc ggagcaggcg ttctacatgg tcggttccat cgaagaagct gtggaaaaag
    25441 ccaaaaaact ttaacgcctt aatcggaggg tgatatggca atgacttacc acctggacgt
    25501 cgtcagtgca gagcaacaaa tgttctctgg tctggtcgag aaaatccagg taacgggtag
    25561 cgaaggtgaa ctggggatct accctggcca cgcaccgctg ctcaccgcca ttaagcctgg
    25621 tatgattcgc atcgtgaaac agcacggtca cgaagagttt atctatctgt ctggcggcat
    25681 tcttgaagtg cagcctggca acgtgaccgt tctggccgac accgcaattc gcggccagga
    25741 tctcgacgaa gcgcgagcca tggaagcgaa acgtaaggct gaagagcaca ttagcagctc
    25801 tcacggcgac gtagattacg ctcaggcgtc tgcggaactg gccaaagcga tcgcgcagct
    25861 gcgcgttatc gagttgacca aaaaagcgat gtaacaccgg cttgaaaagc acaaaagcca
    25921 gtctggaaac aggctggctt tttttacgcg tgacccgtcc tgaagagcgt tcacatagat
    25981 cctgctgata taaaaccccc tgttttcccg tttattcatt gatcgaaata agagcaaaaa
    26041 catccacctg acgcttaaat gaaggtattg ccttaatttt ctgcagacaa aaggcgtgac
    26101 gatggtcgaa aatggcgctt tcgtcagcgg ggataatccg ttattgaaca atttatcctc
    26161 tgtccatttc acgatgaaaa aaatgtagtt ttttcaaggt gaagcggttt aaattcgttc
    26221 tcaaattaca gtcaggacgc gtatgttgaa taatgctatg agcgtagtga tccttgccgc
    26281 aggtaaaggc acgcgcatgt attccgatct tccgaaagtg ctgcataccc ttgccgggaa
    26341 agcgatggtt cagcatgtca ttgatgctgc gaatgaatta ggcgcagcgc acgttcacct
    26401 ggtgtacggt cacggcggcg atctgctaaa acaggcgctg aaagacgaca acctgaactg
    26461 ggtgcttcag gcagagcagc tgggtacggg tcatgcgatg cagcaggccg cacctttctt
    26521 tgccgatgat gaagacattt taatgctcta cggcgacgtg ccgctgatct ctgtcgaaac
    26581 actccagcgc ctgcgtgatg ctaaaccgca gggtggcatt ggtctgctga cggtaaaact
    26641 ggatgatccg accggttatg gacgtatcac ccgtgaaaac ggcaaagtta ccggcattgt
    26701 tgagcacaaa gacgccaccg acgagcagcg tcagattcag gagatcaaca ccggcattct
    26761 gattgccaac ggcgcagata tgaaacgctg gctggcgaag ctgaccaaca ataacgcgca
    26821 gggtgaatac tacatcaccg acattattgc gctggcgtat caggaaggac gtgaaatcgt
    26881 cgccgttcat ccgcaacgtt taagcgaagt agaaggcgtg aataaccgcc tgcaactctc
    26941 ccgactggag cgcgtttacc agtccgaaca ggctgaaaaa ctgctgttag caggcgttat
    27001 gctgcgcgat ccggcgcgtt ttgatctgcg cggtacgctt actcacgggc gcgatgttga
    27061 aattgatact aacgttatca tcgagggcaa cgtgactctc ggtcatcgcg tgaaaatcgg
    27121 caccggttgt gtgattaaaa acagcgtgat tggcgatgat tgcgaaatta gcccgtatac
    27181 cgttgtggaa gacgcgaatc tggcggcggc ctgtaccatt ggcccgtttg cccgtctgcg
    27241 tcctggtgct gagttgctgg aaggtgcaca cgttggtaac ttcgttgaga tgaaaaaagc
    27301 gcgtctgggt aaaggctcga aagctggtca tctgacttac ctgggcgatg cggaaattgg
    27361 cgataacgtt aacatcggcg cgggaaccat tacctgcaac tacgatggtg cgaataaatt
    27421 taagactatt atcggcgacg atgtgtttgt cggttccgac actcagctgg tggccccggt
    27481 aacagtaggc aaaggcgcga ccattgctgc gggtacaact gtgacgcgta atgtcggcga
    27541 aaacgccctg gcgatcagcc gtgtgccgca gactcaaaaa gaaggctggc gtcgtccggt
    27601 aaagaaaaag taattctggc tgggaaatcc ggtcatatgg gatgaggaga taacataatc
    27661 tcccgcccac aagcagtaac tataaaaata accccactct ctacaaggct cggggcgccc
    27721 gaaaaaacgg gcatacaggt tgaccgacaa cgatataaat cggaatcaaa aactatgtgt
    27781 ggaattgttg gcgcgatcgc gcaacgtgat gtagcagaaa tccttcttga aggtttacgt
    27841 cgtcttgaat accgcggata tgactctgcc ggtctggccg ttgttgatgc agaaggtcat
    27901 atgacccgcc tgcgtcgcct cggtaaagtc cagatgctgg ctcaggcagc ggaagaacat
    27961 cctctgcatg gcggcaccgg tattgctcat actcgctggg cgacccacgg tgaaccttca
    28021 gaagctaacg cgcatccgca tgtttctgaa cacattgtgg tggtgcataa cggcatcatc
    28081 gaaaaccatg aaccgctgcg tgaagagtta aaagcgcgtg gctatacctt cgtttctgaa
    28141 accgacaccg aagtgattgc ccatctggtg aactgggagc tgaaacaagg cggtactctg
    28201 cgtgaggcag ttctgcgtgc tatcccgcaa ctgcgtggtg cgtacggtac agtgatcatg
    28261 gactcccgtc acccggatac cctgctggcg gcacgttctg gtagcccgct ggtgattggc
    28321 ttgggtatgg gtgaaaactt tatcgcttct gaccagctgg cgctgctgcc ggtgacccgt
    28381 cgctttatct tccttgaaga gggggacatt gcggaaatca ctcgccgttc ggtaaacatc
    28441 ttcgataaaa ctggcgcgga agtaaaacgt caggatattg aatccaatct gcaatatgac
    28501 gcgggcgata aaggtattta ccgtcactac atgcagaaag agatctacga acagccaaac
    28561 gcgatcaaaa acacccttac cgggcgcatc agtcacggtc aggttgattt aagcgagctg
    28621 ggaccgaacg ccgacgaact gctgtcgaag gttgagcata ttcagatcct cgcctgtggt
    28681 acttcttata actccggtat ggtttcccgc tactggtttg aatcgctggc aggtattccg
    28741 tgcgacgtcg aaatcgcctc tgaattccgc tatcgcaaat ctgccgtgcg tcgtaacagc
    28801 ctgatgatca ccttgtcaca gtctggcgaa accgcggata ccctggcggg cctgcgtctg
    28861 tcgaaagagc tgggttacct tggttcgctg gcaatctgta acgttccggg ttcttctctg
    28921 gtgcgcgaat ccgatctggc gctgatgacc aacgccggta cggaaatcgg cgtggcatcc
    28981 actaaagcat tcaccactca gttaactgtg ctgttgatgc tggtggcgaa gctgtctcgc
    29041 ctgaaaggtc tggatgcctc cattgaacat gacattgtgc atggtctgca ggcgctgccg
    29101 agccgtattg agcagatgct gtctcaggac aaacgcattg aagctctggc agaagatttc
    29161 tctgacaaac atcacgcgct gttcctgggc cgtggcgatc agtacccaat cgcgctggaa
    29221 ggcgcattga agctgaaaga gatctcttac attcacgctg aagcctacgc tgctggcgaa
    29281 ctgaaacacg gtccgctggc gctgatagat gccgatatgc cggttatcgt cgttgcaccg
    29341 aacaacgaat tgctggaaaa actgaaatcc aacattgaag aagttcgcgc gcgcggcggt
    29401 cagttgtatg tttttgccga tcaggatgcg ggttttgtga gcagtgataa tatgcacatc
    29461 atcgagatgc cgcatgtgga agaggtgatt gcaccgatct tctacaccgt tccgctgcaa
    29521 ctactggctt accacgttgc gctgatcaaa ggcactgacg ttgaccagcc gcgtaacctg
    29581 gcaaaatcgg taacggttga gtaatatctc tcgttgatag aaaagggctg ccgaccggcg
    29641 gccctttttt tataagtgat atcaataatt ccattattca tatacccatt gatataaata
    29701 tattctgaat tattgataat gtctgataat aaacttgatt tcttgctgtt ttaataatat
    29761 ttaaataata aatttatatt taagattgat taattaatat tatttttaga taaaactatc
    29821 tcattggtgt actatgcctg tgaatcaaag cacacttatt attttaagtg atgattcatg
    29881 gtaaaggata tattatatca atggagtttt tatgaagcgt aatattatag gcggtgcatt
    29941 cactctggca tctttaatgc tggccggaca tgcactggca gaagatggtg ttgttcactt
    30001 cgtcggtgaa attgtcgaca ctacttgtga agttacctcc gatacagccg atcaaaccgt
    30061 cccactgggt aaagtcagta aaaatgcatt ttcaggtgta ggtagtctgg cgtcaccaca
    30121 gcagttcagt attaaacttg aaaagtgccc ggcaacctac actcaggcag ccgttcgttt
    30181 tgatggtaca gaagcgcctg gcggcgacgg cgacctgaaa gtgggtacgc cgcttacagc
    30241 gggcaaccct ggcgatttta ccggtacagg acaagcgatt gcggcaacgg gtgtcggtat
    30301 tcgtattttt aaccagtcag ataactcgca ggttaaactt tataacgatt ctgcctatac
    30361 cgctatcgac gcggaaggca aggctgaaat gaagtttatt gcacgctatg tggcaaccaa
    30421 tgcaaccgta acggcgggta cggcgaacgc cgattcacaa tttactgtcg aatataagaa
    30481 ataatttatc aagggccttt ttattcagtt ggctttggcc agggagtcgt tatgtccaga
    30541 aaaagttgtt taatcaggct ctttaaaaca accgtttcat gtaagttatt taccgcgatt
    30601 atactcagtg tttttttagt ccaaccagca ttaacctatg caggcgttgt cattggtggt
    30661 acacgggtag tttacttaag taataaccca gataaatcta tttctgtatt tagcaaggaa
    30721 gaaaaaattc cttatctcat ccaggcatgg gtagatccgt ttaataaaga ggataaaagc
    30781 aaagcaccgt ttaccgttat tcctccggta tcacgattag aacccgcgca agaaaaagta
    30841 ttacgcatta ttcataccaa aggtgtgtca ttgcctgatg atcgcgagtc agtcttctgg
    30901 ctgaatatta aaaatattcc gccctctgcg agcaacaaag cggcgaactc actggaaatc
    30961 gcagttaaaa cgcgtatcaa gctgttctgg cgaccggcaa gcattcgcct gattcccgaa
    31021 gatgctgcac cgaaggtgaa gtggcaccgg gaagggcgaa acctgattgc agagaatccc
    31081 aacccgattc atatcagtgt gatggatgtc atcgttgatg gtcatgatgt gcccctcaac
    31141 atgctccgtc cttttgaaac gctcacgtta cctttacctg ctaattctgc gggaggccaa
    31201 atgatctggc gatttattaa tgattacggc gcagtcagtg acccgataaa aatgacgctg
    31261 taagcaaggg aaaaggatag gcgtattatg atgacgacca gaataaaggt tggcctcacg
    31321 gcagggacgt gtctgatttt ctcgcaaagc ctgatggccg aggtcagtgt attcaatccg
    31381 gcgcttctgg aaatcgacca tcaatccgga gtcgatattc gccagtttaa tcgggcaaac
    31441 ctgatgcccc caggtgttta tagcgttgat atttttatca acggtaaaat gtttgaacgt
    31501 caggatgtga cctttgttca ggataatccc gatgctgatt tacacgcctg ctttgtcgct
    31561 attaaaaaaa cgctaaccac ctttggcgta aaagttgatg cgctgaaatc gctcaatgat
    31621 gtagatgaaa cagtctgtat tgatcctggg ccacgcattg aaggctcatc atggcagttc
    31681 gacagcgata aactgcaact gaatatctcc attccccaaa tctatatgga tgcgatggct
    31741 tatgattaca tcagcccctc gcgttgggat gaggggatta acgcgctcac catcaactac
    31801 gatttttctg gttcacatac tctacgttcg gattatggtt cacaagagac agataccagt
    31861 tatctcaatc tgcgcaatgg actgaatatt gggccgtggc gattacgcaa ttacagcacc
    31921 ttaaatacga ccgatggcag tgcagaatac aactctataa gcacctggat acaacgtgat
    31981 atagctgcgt taagaagcca gattatgata ggtgatacgt ggacggcgag cgatatcttc
    32041 gacagtacgc aaattcgtgg cgcacgcttg tataccgata acgatatgtt gcctgccagc
    32101 caaaacggat ttgcccccgt agtgcgtggg attgcaaagt ctaacgccac tgtcattatt
    32161 cgccagaatg gctacgtgat ttatcagtca gccgttccac aaggtgcttt tgaaattacc
    32221 gacctcaaca ccgccagtac aggtggtgat ctggacgtaa ccatcaaaga agaagacggt
    32281 agcgaacaac gatttactca accttatgcg tcgttagcca tcctcaaacg tgaaggtcaa
    32341 actgatgttg atgtcagtgt gggagaatta cgcgatgaag atggctttac ccctgacgtt
    32401 attcaggctc agatccttca tggttttccc tacggtttca ccttgtatgg aggtatgcag
    32461 gctgctgaaa agtatggttc tgccgctttg ggtgtcggta aagatcttgg cgcattgggc
    32521 gcaatttctt tcgatgtgac acatgctcgc gcgaaattta gccatgatga tacggaaacc
    32581 ggtcagtctt atcgcttcct gtactcgaaa cgctttgacg atacggatac cagcttgcgc
    32641 ctggttggct atcgttactc caccgaaggt tattataccc tcaatgaatg ggcgtcccgg
    32701 cgtaataatc ctgaagattt ctgggaaacc ggtaaccgcc gcagccgtgt ggaaggaacg
    32761 ttaacgcaat cactgggcag agattatggc aacttatatc tgacattaag tcgccagcag
    32821 tactggcata ccgatgatgt tgaacgatta atgcaatttg gctacagcag tagctggaag
    32881 cgtctctcgt ggaacgtctc ctggagttat tccaatactg cccggcaggg gacggggaac
    32941 aaccatgcca gtgataacac cagtgagcag atctacatgc tctctttatc tgttccttta
    33001 tcgggctggt gggggaatag ttacgccacc tattctgttt cacaaaacga taattccggt
    33061 agctcacatc aacttggact cagcggtacg gcgctggaaa gaaataacct ttcctggaat
    33121 ttaatgcagt cctataacag ccatgatgat gaggttggcg gtaatatgtc cctgacctat
    33181 gatggcactt atggcacggt gaacggcagc tataactaca gccaaaattc tcagaggctg
    33241 aattatggta tcagaggggg aattctggct cacagcgaag gcgtaacgtt aagccaggag
    33301 ttgggtgaaa ccatagctct tgttaaagca ccaggggctg ccgggttaga aatagacaat
    33361 atgcgtggtg ctgcgacgga ctggcgtggt tatacggtca agacacagct aaacccttat
    33421 gatgaaaacc gggtagcaat cagcgataac tatttctcga agtcgaatat agaacttgat
    33481 aataccgtcg ttacgatggt tcccacccgt ggcgcggtgg ttaaagccga atttgttact
    33541 cgtgtaggtt atcgtgtgct tttcagggtg gcgggtacaa aaggtaaacc cgcacctttt
    33601 ggcgctattg ctacagtaca aaatacaagc tccgctgatt cagggattgt cggtgacctg
    33661 ggggagcttt atctctctgg ccttcctgaa aaggggcaag taatgctctc ctggggggaa
    33721 aatgccgcca ctacatgcac cttcgattat tcaatttcaa taccagaaag tgaaagcggc
    33781 ttaattgaac aaggtgtgac atgtcattaa aaggattttt atgaacaagt atattataca
    33841 gtggtgcttt actgttttta tcttctcctt tagtggtgca acatttgcgg ccccaaaagg
    33901 tatctgcacc tcagataatg gggcctttca tagcacactt gatttttccg gctatctgat
    33961 tacggcagac gagaacagag tgggagagac ctttaataaa accgtgacaa atggggactc
    34021 ttatcctgct cactgccatt gtgatacagg gaaagtgggg gagtttcctt atatttacta
    34081 tacagcgaga ataaacgagg ccttaagtta tgcgggtgtt cgttctaatg taaactactt
    34141 taatttgaat cctaatctgg atgttggaat atcgatagac cttctcggtg taggatacat
    34201 taatgcacct tttgaatacc atgctaacag gcctactgga ggttcatata aatgtagtcg
    34261 cacggaccca ttaagtattt ccagtggtgc aaaagcaata atttattttt atattaagaa
    34321 gacttttgct ggaaagttaa ttgtccctga aacgctcgtg gcaaaattgt acggaactat
    34381 aagccgtgac actccggttg attactcaca acctatggcg gatgtttata ttcgtggcga
    34441 tatcactgca ccgcaaagct gtgaaattaa cagtttaaga cccattaatt ttgattttaa
    34501 agaaattcct gcagcagatt tttcttcggt agctggaagt accgtgacaa cgcataaaat
    34561 taccaaaacc gtcaccattg agtgtgaaaa tttaggaata ctaaatactg atgatatcag
    34621 tacctctttt tatgctaccg aacccagtac tgacaactca atggtcgtga catcaaaccc
    34681 gaacgttggg ataaaaattt atgataaaaa taataaggaa atcaacgtta acggtggtga
    34741 actgccgaca gatatgggta aatcaaacgt ctttggtgaa aaagccggta gcgtcacttt
    34801 ttctgctgct cctgcaagcc tcacgggggc tcgccccgcg ccaggaacat ttaccgcgac
    34861 tgcaacgata acaattgaga ttgtacgcta aacgaactga cagaggtgtt cctcacaccg
    34921 ttgtcatctt acgcattttt gtccgcgagt tttcactttg catgacaaaa agatgacact
    34981 acttttcttt ttcttagtca ataaaatcaa aaagttaatt aataattctg atttgtcata
    35041 aaactgtcat aaaccttaca tataactgtc acctgtttgt cctattttgc ttctcgtagc
    35101 caacaaacaa tgctttatga atcctcccag gagacattat gaaagttatg cgtaccaccg
    35161 tcgcaactgt tgtcgccgcg accttatcga tgagtgcttt ctctgtgttt gcagaagcaa
    35221 gcctgacagg tgcaggtgca accttccctg cgccggtgta tgccaaatgg gctgacactt
    35281 accagaaaga aaccggtaat aaagttaact accagggtat cggttcttcc ggtggcgtaa
    35341 aacagattat cgctaatacc gttgattttg gtgcctctga cgcgccgctg tctgatgaaa
    35401 aattggctca ggaaggcctg ttccagttcc cgaccgtgat tggcggcgtg gtgctggctg
    35461 ttaacattcc agggctgaag tctggcgaac tggtgctgga tggtaaaacc ctcggcgaca
    35521 tctacctggg caaaatcaaa aagtgggatg atgaagccat cgccaaactg aatccgggtc
    35581 tgaaactgcc ttcacagaac attgctgtcg tgcgccgtgc agatggctcc gggacatcct
    35641 tcgtcttcac cagctacctg gcgaaagtga acgaagagtg gaaaaacaac gttggtactg
    35701 gctctaccgt aaaatggccg atcggtctgg gcggtaaagg taacgacggt atcgccgcgt
    35761 tcgttcagcg tctgccgggt gcaattggtt acgttgaata tgcttacgcg aagcagaaca
    35821 acctggcgta caccaaactg atctccgctg atggtaaacc ggttagtccg accgaagaaa
    35881 acttcgctaa tgcagcaaaa ggcgcagact ggagcaaaac attcgctcag gatctgacta
    35941 accagaaagg cgaagatgca tggcctatca cttctaccac gttcattctg atccataaag
    36001 atcagaagaa acctgagcag ggtgtagaag tgctgaagtt cttcgactgg gcgtacaaaa
    36061 ccggggctaa acaggctaac gacctggatt atgccagcct gccggatagc gtagtggaac
    36121 aggttcgcgc tgcgtggaag accaatatta aagacagtag cggcaagccg ctgtactaat
    36181 aaaactccag gccgggtacg gtgttttacg ccgcatccgg cattacaaaa tgactttgta
    36241 aacgcgttta actgaagagt aacttatggc tgcaaccaag cctgctttta acccaccggg
    36301 taaaaagggc gacataattt tcagcgtgct ggtaaaactg gcggcgctga ttgtgctatt
    36361 gatgttgggt ggcattattg tctctctgat catctcatcc tggccgagca ttcagaaatt
    36421 tggtctggct ttcctgtgga ccaaagggtg ggatgcgccg aacgatatct acggggcgct
    36481 ggtgccgatc tacggtacgc tggtgacctc gtttatcgcg ctgctgatcg ccgttccagt
    36541 gagtttcggt atcgccctgt tcctgaccga gcttgcgcct ggctggctga agcgcccgct
    36601 gggtatcgcc attgagctgc tggcagccat tccaagtatc gtttacggca tgtggggctt
    36661 gtttatcttc gccccgctgt tcgccgttta tttccaggag ccggtcggca atatcatgtc
    36721 gaatatcccg attgtcggcg cgctgttctc cggcccggcc tttggtatcg gtatcctcgc
    36781 ggcaggcgta atcctcgcca ttatgattat tccgtacatt gcagcggtaa tgcgcgatgt
    36841 attcgaacaa accccggtga tgatgaaaga gtcagcctac ggtatcggct gcaccacctg
    36901 ggaagttatc tggcgtatcg ttcttccgtt caccaaaaat ggggttatcg gcggcattat
    36961 gctgggtctg gggcgtgcgc tcggtgaaac catggcggtg acctttatca tcggtaacac
    37021 ctaccagctc gacagcgcct cgctgtacat gccgggcaac agtatcacct ctgcgctggc
    37081 gaacgaattt gcggaagcgg aatccggtct gcacgttgct gcactgatgg aactgggcct
    37141 gatcctgttt gtgattacct tcatcgtcct cgccgcatcg aagtttatga ttatgcgcct
    37201 ggctaagaat gagggggcac gctaatggct atggttgaaa tgcaaaccac tgcggcgctg
    37261 gctgaatccc gccgtaaaat gcaggcgcgt cgccgcctca aaaaccgtat tgcgctgacg
    37321 ctctcgatgg cgacgatggc tttcggtctg ttctggctga tctggatttt aatgtccacc
    37381 atcactcgcg gtatcgacgg tatgtcgctg gcgctgttca ctgaaatgac gccgccgccc
    37441 aatacggaag gtggtggtct ggcgaacgcc ctggcaggta gcgggctgtt aattttgtgg
    37501 gccacggtat tcggtacgcc gctgggcatt atggcgggga tttatctggc ggaatatggt
    37561 cgtaaatcct ggctggcaga agtgattcgc tttattaacg acattctgct ctctgcgccg
    37621 tcgattgtgg ttggtctgtt tgtttacacc attgtggtgg cgcagatgga gcatttctcc
    37681 ggttgggcgg gtgtgattgc cctggcgttg ttgcaggtgc cgattgttat ccgcaccacc
    37741 gagaacatgc tgaaactggt gccggacagc ctgcgtgaag cggcttatgc gctgggtaca
    37801 ccgaagtgga agatgatctc cgcgattacg ctgaaagcgt cggtgtccgg gattatgacc
    37861 ggtatcctgc tggcgattgc ccgtattgct ggtgaaaccg cgccgctgct gtttaccgcg
    37921 ctctccaacc agttctggag cacggacatg atgcagccga tcgccaacct gccggtgacg
    37981 atctttaagt ttgcgatgag cccatttgcg gaatggcagc aactggcctg ggccggggtg
    38041 ttgatcatta ccctgtgcgt cctgctgctg aacattctgg cgcgcgttgt atttgcgaag
    38101 aataaacacg gttaacactt tgccggatgc ggcgtgaacg ccttatccgg cctacggtaa
    38161 gcctgattcg cgaagtgcat caggcacgat gaggaaagat tgcaatgagt atggttgaaa
    38221 ctgccccgag taaaattcag gttcgtaatt tgaacttcta ctacggcaaa ttccatgccc
    38281 tgaaaaacat caacctggat atcgctaaaa accaggtaac ggcgtttatc gggccgtccg
    38341 gctgcggtaa atcgacgctg ctgcgtacct tcaataaaat gtttgaactg tacccggaac
    38401 agcgtgcgga aggtgaaatt ctgctcgatg gcgacaacat cctgaccaac tctcaggata
    38461 tcgccctgct gcgtgcgaaa gtgggcatgg tgttccagaa accgacgccg ttcccgatgt
    38521 ccatctacga caacatcgcc tttggcgttc gtctgtttga gaagctctcc cgtgccgaca
    38581 tggacgagcg cgtgcagtgg gcattgacca aagccgcatt gtggaacgaa accaaagata
    38641 aattgcacca gagcggttac tctctctctg gtggtcagca acagcgtctg tgcattgcgc
    38701 gtggtatcgc cattcgcccg gaagtgctgc tgctcgacga accatgttcg gcgctcgacc
    38761 ccatctccac cgggcgtatt gaagagctga tcaccgaact gaagcaggat tacaccgtgg
    38821 tgatcgtcac ccacaacatg cagcaggctg cgcgttgttc cgaccacacg gcgtttatgt
    38881 acctgggcga attgattgag ttcagcaaca cggacgatct gttcaccaag ccagcgaaga
    38941 aacaaacaga agactacatc accggtcgtt acggttgatt caggagtgcg ttatggacag
    39001 tctcaatctt aataaacata tttccggcca gttcaacgcc gaactggaaa gtatccgcac
    39061 gcaggtgatg accatgggcg gcatggtgga gcagcagctt tctgatgcaa tcaccgcgat
    39121 gcataaccag gacagcgatc tggcgaagcg cgtcatcgaa ggcgacaaga acgtcaacat
    39181 gatggaagtg gcgatcgatg aagcttgcgt gcgcattatc gccaaacgtc agccgacggc
    39241 gagcgacctg cgtctggtta tggtgatcag taaaaccatt gccgagctgg agcgtattgg
    39301 cgacgtggcg gacaaaatct gccgtactgc gctggagaaa ttctcccagc agcatcagcc
    39361 gttgctggta agtctggagt cgctgggccg tcataccatc cagatgctgc acgacgtgct
    39421 ggacgcgttc gcgcggatgg acattgacga agcggtacgt atttatcggg aagataaaaa
    39481 agtcgatcag gagtacgaag gtattgttcg tcaactgatg acctacatga tggaagattc
    39541 gcgcaccatt ccgagcgtac ttaccgcgct attctgtgcg cgttcaatcg aacgtatcgg
    39601 cgaccgctgc cagaatattt gtgagtttat cttctactac gtgaaggggc aggatttccg
    39661 tcatgtaggc ggtgatgagc tggataaact gctggccgga aaagacccga aagagtaacg
    39721 ctaacagtag gtcagataag gcttttacgc cgaatctggc aatcagcccg gtcatacata
    39781 gctaccgggc tgataaactt tctccctgaa atattgccac acaaactcac ctcgaaactc
    39841 tccccagtgc aatgcaaata tattatttca tttgaattat tttactttta atgtagtgcc
    39901 ccgttataaa attttttttg ttattccttt catttatatc gttacccgca cagaaaatgg
    39961 aaaagaaaac aaaaaaccag ggtaaaagcc ctatatcgga acataaaaaa tgagcgattt
    40021 tttacagtat tcataggtgg tttagtcata atcgtatcaa aacaacacca aagtgttctt
    40081 ttttatacaa aacggttatg tattgtgagc gggcgaataa atattgtgtt caggagaaat
    40141 gattatggct atatcatcat atagtacagt tacagataaa tttgctactc tgactaaaat
    40201 gccagatgaa aatctgaatg aaaaaaatag aacgcaaaaa tattcaggaa aaattggttt
    40261 gtctcttgat gatgtgggta agtattcagt aactcataaa gatataacaa acattttagc
    40321 tggtgatagt caatcttttc ttaaacgaag tctttgggag tttatactcg atctttttcc
    40381 catgtctcat attaaggagg ctaaacaagc cattgtagat tttgtgacga aaactgatga
    40441 taaagttgag atgtttaata gagttaaatc tctcgccgat caagatcaac aatggcgctt
    40501 taatacgact acagaattta ctgtagatga aaataaagac attattgtta gtagagtatt
    40561 taatataaat attaatgcgc aagattatca taatgatgct gaaaaaaaca caatgcgttc
    40621 tttattttct gaagaactca gcttggatat ttataagcat gatattcatt ttgatcagaa
    40681 ggcttttcaa tcagggatgc tgaaaataca gttcccaaat gataatgaaa agcttagcgt
    40741 atatttaaaa aataaaatcc cctttgaaaa tatcacaatc gatttatctg cgctggaaaa
    40801 agaagccctg gcatctctaa aatattgtaa ctttaaaaaa gtgacattca ctggtgctat
    40861 atcttacgaa aatcttaatg gttcagtatt tggaaattgt ttttttgagg actgtatttt
    40921 tgagaatgtg tcacttgtta gctccaggta tcgagtaaaa ccaaataatg aaatacctat
    40981 ttacggcgtg tttaaagagt gttttctgta tcgatgtgaa ataaaaaatt ttaacataga
    41041 tacatcaaaa atatactctg taaatcaaga tcccaatgtg ggtaagccag ttggtgcata
    41101 tctgtttatg cagtcatttg tagatgagtg tactcttcag gatggagtgt gtaaaaatgc
    41161 aagtgttatt gcaagcagtt tgttatgttg taaaatagca aagcttaatg gtgctgaaat
    41221 ggatttcata gggacttcgt tttatcacaa atataaatgt gaattaaaat atgataatca
    41281 tttttataat tgtgatttta gctcctctga tatgacatat aagcgaaatg gaattcgcaa
    41341 agatttactg gattttgatc ccagagagta tttcaaaaag gatggaggta aacttaatct
    41401 ggataaacgc atttctgacc ttatctacat taatcttttc cctaagatga gtggcggtcg
    41461 ttatataaat gataaatacg ttgctggtga tatagaaaaa tatttaaaac ttagtaattg
    41521 caatttactt gaaacttgtt tgggcgagtc aatcaatgga attaccttga atgattgtgc
    41581 tattgatcat ttaaccactt ggttaagtgg taatcttgct gcgaacaccc agattagaat
    41641 tcctgtttat atggacatga aaggagctat agctgaaaat ggcatttcag atttcatgaa
    41701 aaatttaatg gaaattaatt catgggtaga agaatttaat aatcatggca aggatgcgga
    41761 aagttattac caaaaggaac tattatattt acaatctttg ttggtcaagt tggggcgctt
    41821 caatcttgat gatgaaggaa aaaaatatca acttaatggt gaagctatcc agtatattat
    41881 attaaaagtt ttgtacaata tagaaaaaga tatagccaat gaaatagata ttgataaagt
    41941 gaatttcatg cataagctct atactggatt cttttggcaa agaccttacg atcaacgtaa
    42001 ctatttaaaa ataaatcgtt ctgctatttc aaacagcaaa acatttcact tccactttga
    42061 ttttaatgat ttgcgaaata tttacaaaga atataataaa aatcataaag atgatagttt
    42121 tgataaaatg caaaagattt ttttagcatt gaatgaattt aaggaaagaa cgaaaataaa
    42181 ttatagggat tatagaaaag tatcaaaata tgtagatgat aaagtcgatt tattctctgg
    42241 cattgagcaa tacttacggg aagtaattga gaaaaacaat agctataata aggaaaatta
    42301 tactgctaaa aaacaaaaag aagccgacct ttttatgcta agaaataatt tagtcaaaac
    42361 taaggctaaa ttgagtgagg agagctgtat gttaaataaa gaagataaaa aagatgcagc
    42421 caaaattcgt aagattaatg aaacactaaa taaaatagat gacgaaatag caacaataga
    42481 taaagagatt gtaaagctta aggatgaaac agagagattg gagaaagagt atgaacaaga
    42541 aaatacactt aatgatgtag ttcaaaatat caggagttgg ctaaaagaaa atcaaaatat
    42601 ggtgaaagct attaagaaaa ttgatactga ataacatggc gaataaataa acggatcaag
    42661 tcgttcctgt gacaatgagg ttatacaaaa agacgcttac tcatcagaaa tctggtatgc
    42721 tttaattaaa ccagtgtcgc tgatgtgtaa gcgttgacgt tgggaggcga ttaaatggtt
    42781 ataccactcc tacgcctttt ccagacggcg tataagagtg caacatgttg agttttccga
    42841 gtgaatggtg cattttacta accgagccat ttgaatcaac caacgcaata aaaaagactg
    42901 gaataataag cctgtattta ttattcatcg caatcgcact ctacggtttt atcctgcaga
    42961 aatgagacta cttccagcgg ttgtggatta tccctgggcg atagtcatag tctggtgtca
    43021 ggattcacaa atgacctgtt acctgacatg gatataaaca caacatagag tgcggtagtt
    43081 aatctgaatc atctattttt cggctacaca gcacaaaaca acattattga ttctttataa
    43141 gagagaatct ctccatcatt tttatataaa tcattattaa gaaaatccag aagctcttgc
    43201 cttacactat aaatattggc tttttggtca accaacgaca gtaatatatc cttgatacat
    43261 tttaatttta tatctctatc agatattgta ttaagtgtga agatgatatt acgtttatta
    43321 gaactggaga accaatattt tagagtatta ttcgtccact ttgttggtgt cttaattagt
    43381 tgtgctgttg ttgtttttac attgttaaat gtagaaccgc taaaatttgt cccgacgcac
    43441 gaaatgaaat tcatttctga attgtcaaaa ttgcaattct ttatctcaca catatcaaaa
    43501 ttaatatcat gaaatgtcga acatttcatg tttgcatgtg aaaataaaca tgcaataaac
    43561 gttatatatt gcaaactgga atgacaaaaa gatttatttt taaaatcaaa agaacaggtt
    43621 tttttgactt tgaagttgtt attatatctg caatgtaaag aaatcatgtt ttttattaac
    43681 tgataacacc actgtgatga ttctggtaat tcattggtgt ataaaaggac gttttcatcg
    43741 aatataacct gtgcattatg gttatttatt gaagatatat ggaagctatc atttgttaaa
    43801 gctatggttg tgctaaaatt atgatggtga tcatagctca tgaaatcaaa tgtaacaatg
    43861 tcgttgtttc ctttcttttc tttgtgatat gcgtttaaaa aagaaatatt ttcgatctgt
    43921 tcatcagtta atgtaaatgg catttcgtca tcaaagaaag cataatcggc atttgggact
    43981 ggaatatttt caattgagca tagttttaat aaaaaaatat gaaaacaatg aaacttattt
    44041 ttgtcaataa gagaacttaa tctctgttgt atcttctgta gttgtccttt gggaagtaaa
    44101 tgctgtaaat ttgaaaaaga gccatctaaa acatatttgt atgtaaaaag gtcatctctt
    44161 ggaccattta ttaaaaaatc gtcagacaat ttagaaaaac gattgagatg acataaatat
    44221 tccatttgct ccatgtctat atacttatgg ggaactgcgt ggtttggtgg tgtgccaata
    44281 agattatcca taaatcacct tgataaaatt taataaaatc tatttatttt aacctcgaca
    44341 ataaaagtgg ctataaaaaa acgctcattt tctgttgctg cggttaaccc gtggaatgta
    44401 ccgttaaccc ccaaaaaatt gcaaatccag ggtgaacacc ctgggttaaa atggaaataa
    44461 tgagctattt tttacagtat tcaccggtaa tataagtaac acttgttgct aacctaacta
    44521 gcagtacagt taattaacaa catagaagtt attgtttttc aaacaactca atattgcacg
    44581 ccggaggatt tatggtcgct aaattaaaac cagatgtttt tgttaatact aatccttttt
    44641 tagaagcaat gtataaggag aggcttagtc ataaaggcta ttctgataaa attgctttaa
    44701 gtattaatga taagaaatat aatgtaaact ctaaggatat tgaaaatatt cttgatggca
    44761 aaggggattt atttaaaaaa cgtacactgt gggagtttgt tcgcgatctt tttcctggat
    44821 ctcatattaa agaagtcaaa ggtttaattt acgaatttgt cacgaaagtt gataataaag
    44881 ccgaggtgtt cgataagatt aaatctctgg caaaaaaaga acaacaatgg cgatttagta
    44941 ctaagacaga ctttaccaca aatgaaaata atgaagttat tgtgagtaga tcctttaatc
    45001 tctacacggg ggctacttcg aatgataacg aaaaaaaaca ggtttcttca gagagattaa
    45061 cgcttgataa ttatattgat gatttacatt ttgataattc tcccttgaag agatttacgt
    45121 ttgatgatta tgctgtaaaa cttacgacac tgattaaaaa taaaattcct attattaata
    45181 caacaatcaa tttatcatcg ttgtctaagg atgttttaaa ttcattaaaa tattgtagtt
    45241 ttaaaaatgt aattttttca ggagtgataa acagccctaa ccttgaaggt ccggtttttg
    45301 aaaattgtta tttcgaggat tgtaaattca acaacataca gctttatgag actaatgatg
    45361 atactgtggg gtcaggaaat aaaaaaccaa taaaaggtat gtttaaaggt tgttttattt
    45421 ctaaatgcaa aattgaaaat tatagatgtg agacgtctaa aatctacacc gttacacaac
    45481 ctgatagtat tcaagaaaaa ttaggttctt atctctttat gcagtctttc gttcaggatt
    45541 gtataattca gggaggacgt tgtcctggtt cgagtatact tctgagtcat ttttataact
    45601 gtacgatagc gaaacttgat gctcatggga tggatttttt ggcaaacgca ttcaataagt
    45661 ctggtgataa cgaaaccagg gcgcaggatc agggaactgt gtttgattgt tgtaatttga
    45721 atagtattaa aatgaactgt ggtcttggta atgtgagttc agatgaatat aagtttaacc
    45781 ccgaagagta tttttctgat tacattgaaa gaagatcgag aaatcttaag ttggatgata
    45841 atttttctaa ctgcgtagag aatatcgaga aggctgttac aacttcattt agcaaggaaa
    45901 attcagagaa tgataatatc tcctttaatt gttgtgattt gaggagggcc aatttagaca
    45961 attattacag agaagatttg tgcaaagact gttctattga tccagattca atttatttag
    46021 aagatggtgt tacaccgata aaatatattt atatgaatct ggacggagct ataagtaaag
    46081 accaaatacc taatttctta aaaaaggtaa gtgctatcaa tagcgatgta aaagattttt
    46141 ataatactga gctaaagatt aaggaagaat ataataacgc attttcagat ttagagtcgt
    46201 ttttgtctac actttatgtt gaaaataaat cgtacaaaga gaaatatcat tttaataacg
    46261 gtagggctga gttttttatt ttcaaagata tgcagtataa agcacaaaat atcattacta
    46321 atatgataga aagtgaccga attaagttta ttgaatctat cataaaaaaa atcattccac
    46381 cggcagatgt aaaaatgcct actaaagatt cagacaaatt tattcgacaa aaacagcatg
    46441 agtctcataa ccaatttgat attgattata aattgaatta tggtgacctt gtcccccttt
    46501 ttgacggtgt tgaagaaaaa caaattgaag tgctaaaaat ccaacttgaa aatataaaaa
    46561 atttcaaagc atattacgac tcagaactca ataagtttgc ttccgatttt cgaaattttt
    46621 ccggtgcatt tgcaattaac tgggaggatt tactaaaaaa ctatcaggaa ataagagctt
    46681 ccattaaaga ttacgatgac ttattgcgag aaattaaaga gttaaccata agtcgggata
    46741 aatcattaga ggagaaaagt actttatttg ataacaaaag ggacaagtgg aatagtcaag
    46801 aagtacagga tgaagtaaag gcattaaata agaagattgc tgactgtgac aatgagatac
    46861 gttctaatct taatattgtt caaaatttta gacgcgagaa tcaatataaa gctaataaaa
    46921 aaatcagtaa cgcgatgctt aatattctgg attggttcga acggtatccc gatattgtga
    46981 aaaatataac ccaggcttaa ccatacagag ggtgagcgac tggacgatta acgacataaa
    47041 ttatttacag ggaatagacg cttactcatc agacacctgg tattttttat ctcaaaccag
    47101 cgtgactgat gtgtaagcgt tgaagtaagg aggcgattat cgcgttatat tccagccgcg
    47161 tgccttccag agtgcaggca gctgcgccag ctcggtaaat gtggtgactt tcgggtgaac
    47221 gatcggttta ttgtgcgggt cagcgcagaa gtaaaacact tccattccgg catctatccc
    47281 tgattgcgca ccggcgctgg agtcatcgac cagaatgcag ttttcaacgt tcacattcat
    47341 cgcttttgcc gcatggaaca ttaacgccgg gtcgggcttc caacgctgga tatcgtagcc
    47401 gctgaacagt ttttccggga agtagtgcag catcttgagc ttacccagcg aatgctgcat
    47461 tttgttgatc ggaccgttag agacgacgca catcggcacg ttaatgctcg ccagcaattc
    47521 gttcgctcct gcgatgtcct caagttcggt atcaaacaga cgcgccactt ctgcgcggta
    47581 aacgtgctcg gcatcggcct ggatcatcgt cacgccctgt tctttactga tgatgtcgat
    47641 gatttcgtag agttttacgc ctttaaaacg ggtaaaaatc tcctccaggt caacgtgaat
    47701 gccaaattcc ctgaacatcg tgacataggc gcgagagcaa atgacctcgc tatcgaccag
    47761 cgtaccgtcg cagtcgaaaa ataccgcttc tatctgggac atgtctttcc tttatcgtta
    47821 gtttaacgtt ttcgtcatgc caattcgcat gacgcaatcg ttgccgtata agcaaataca
    47881 atgaaaaaaa gttatcttca gccgtccata ttgtctcatt ttggtatacg atagcgacgg
    47941 attttccctc cttgttcgga aatagataat gagtcaacaa cacacaaccc aggcttctgg
    48001 ccaggggatg ctggaacgcg tgtttaaact gcgcgaacat ggcacgacgg cacggaccga
    48061 agtgatcgcc ggttttacca ccttcctgac gatggtttac atcgtttttg ttaacccgca
    48121 aattcttggc gttgctggca tggataccag cgccgtcttc gtcactacct gtctgattgc
    48181 tgcattcggc agtattatga tgggactgtt tgctaacctg ccagttgcat tggcacccgc
    48241 tatgggcctg aatgcgttct tcgcttttgt ggttgtgcag gcgatgggct tgccgtggca
    48301 agtcgggatg ggcgcaatct tctggggcgc gattggtcta ctgttactga cgattttccg
    48361 cgttcgctac tggatgatag ccaacattcc ggtaagtcta cgtgtgggca tcaccagtgg
    48421 tatcggtctg tttattggca tgatggggct gaaaaacgca ggtgtgattg tcgctaaccc
    48481 ggaaacgctg gtgagcatcg gtaacctgac ttctcacagc gtgctgctgg ggatcctcgg
    48541 cttcttcatc atcgccattc tggcctcgcg caatattcac gcggcggtgc tggtttccat
    48601 cgtggtgaca acgctgctgg gctggatgct gggtgatgtc cactacaacg gcatcgtttc
    48661 tgcgccgccg agcgtaatga ctgtcgtggg tcatgtagat ttagccgggt cgtttaacct
    48721 cgggctggca ggggtgattt tctctttcat gctggtcaac ttgtttgact cctccggtac
    48781 gctgattggc gtgaccgata aagcaggtct ggctgatgat aaaggtaaat tcccgcgcat
    48841 gaagcaggcg ctgtatgtcg acagcatctc ttcggtgacc ggttcgttta tcggtacttc
    48901 ttctgttacg gcgtatattg aatcttcttc cggcgtttcc gttggcggtc gtaccggtct
    48961 gacggcagtg gttgttggtc tgctgttcct gctggttatc ttcctgtcgc cgctggcagg
    49021 gatggtgcca ggctacgctg cagctggtgc gctgatttac gttggcgtgt tgatgacctc
    49081 aagtcttgct cgcgtgaact ggcaggatct tactgaatct gttccggcgt ttattaccgc
    49141 tgtgatgatg ccgttcagct tctcgattac tgaaggtatc gcgctgggct ttatctccta
    49201 ctgcgtgatg aagattggta ccggacgtct gcgtgacctt agcccgtgcg taatcatcgt
    49261 tgcgctgctg tttatcctga agattgtgtt tatcgacgct cactaaaatc gatagcgcgt
    49321 taaaaacccg ccatgcatga ttgctggcgg gttttttatt tagatcttaa ctcgctgaat
    49381 aaactcacca aatgcggtca attgcccggt caggtggtcc agcgtaccct gatcaatcac
    49441 ttctcctgtt tgcggatcaa ctttgttctg aatcacgccg cccataaatt ccggcttgtt
    49501 catcaccatt gcatcgagga aaaccagaat ctggcgcagg tgatactgac agcgcgcgcc
    49561 gccaatcacg cccattgagc tggtctgaat caataccggt ttaccagcca gcggttgatc
    49621 cggcaggcgg gaaagccagt cgatggcatt tttcagccca cccggtaccg agtagttata
    49681 ttccggcgtg acgatcacca caccgtcagc ctgacggatc tgttccgcca gagcttcaac
    49741 cgttgctgga aaaccgtctt cctgctgtac gtcagcgtca tacaggggaa tgtcggcaat
    49801 ggaaggtaac gcattgactt ccatgctcgc cggagcaatt ttcggcaggg tacgtgcaac
    49861 catgccatta aatgagcctt tgcgcaggct ccccagtaac gtaaccacct gcaatttttc
    49921 agacatgatg actcctgtgg ttgtctatca atatggcgaa aaagccttca cggtagagag
    49981 ctgggcaaaa cgcatcatcc ggctggtagg attgttggcg cggctgtgaa tatcgggcag
    50041 gcttttccag ccgtgtttgc catctaactc ccagacactg agcttttcaa tcgtgggcgt
    50101 gaccgacacg gaccacacca gcacgtcctc cgcgtcgcat aacgcttcta catgatttac
    50161 atgagcacat ttcaggcgtc cggcaatggg cagtagttgc agatcgcgcg gatcgtcatt
    50221 caaaacatca ccggttagtt ttagcacgct tcggcgcagc gtgatgagct gcgcccgcgc
    50281 ttcgttagga tcgttttgtt tactgatcca tagcgattca ttgctggaaa aggtgtggtt
    50341 atcgggcgcg tgtgggctat gaaacccgcg cgtcgcacgc tgcagttcca tatcgaggcc
    50401 acattcacct tcggttgtta acgccacgcc gaccatattc ccggcatagg aaatagaaaa
    50461 cgaaggcaaa tttttatcgc tgaaaaccgg ttttcctttc ggcagggtga cgatttccgg
    50521 caattcgcca atgccataca gcatgaacat cagttctgcg agtaaacctc tggacgccag
    50581 aaaacggctt tgacgatgag ggggaatgtt tcgggcttct tgatgacact gggaggggag
    50641 acgaacagaa atcagatgtc cttccgttaa aatccctcgg gcaaagtgag tcgccatttt
    50701 tcgctccatg cttaatggtc cattatgaat acaagagtaa tcatttactg gaatgatgga
    50761 cagactaaag cgactaaaag ttcgattcag ataagtttat tacaactttt ttacatttca
    50821 ttgtgaaatt gggtcgcgtt tcatacgatt cggttatgcc atcgacgcgt aaagattttt
    50881 aatggtttcc cgtaaccaga cgatcttcgg attacggctg ttccgtttat gccacagaag
    50941 ggtaaaaggg acttccagct ttttttgctg gatttcatca aatgggagag gaagagcaac
    51001 taacggcagt tgatggagtt gattgtagta ctgacagtag cgcggcgcgg tcgccagtag
    51061 tagattgtcg ggttgcgctg ccataaacag tgactgctcg aattccggca ggctcattgc
    51121 aatagtacgt tcgcgcccca gctcctgtaa cacattgtcc agcgcccagg tatcgctctg
    51181 ttcccagcaa atgctgatat gcggataacg taaaaaggtg tccagattcc atgcttcatg
    51241 cagtgccgga tgatctttgc gtaaccagac gcagggcaca tcactaaaca gcacttcata
    51301 atcaatggct aacggtagcg agcttaacag ctcccgcgaa cggggatgac tttcgcgacc
    51361 ggaaaaacca atatccactt caccacgagt aatggcatct aaggaatcgt aatcccagtt
    51421 acgtaatttg atggtcgcct gcgggtaacg ttggtagatc cgtttcgaca gcgcattaag
    51481 catgatcatc attagcggtg attctgctgc cagctcaaac ttcaggccgc gcggtgtctg
    51541 gtggtgcggt ttatccagca gcaggttgct catttgcatc cactccgcca gattttgctc
    51601 catgctgacc attagcggtg tgggcgacag ccccagcggt gagttcacaa agagcgggtc
    51661 gtcaaaccac gctcttaact ttgccagcga cttactcacc gccgaagggg tcacgttcat
    51721 ccgcttcgcc gctttggtca cgctacgctc ctgcatcagc agttgcagac agagcagcag
    51781 attgagatca agcgtggtga tggatttctt catgagcggc aaccgggcgt ccaggcgcga
    51841 cgaacataat cagcaacagg ctcactatgc tacaggcaat cagaatcccg atcagcatat
    51901 tccatgcgcc gataccaacc accgccgcca gccaaatcca cagtgacgaa ccgcaaactt
    51961 gcgcaatacc taaggtcgag ctggctacgc ccgcgcgtaa tgaaaacggc cctaacgcct
    52021 gactcatcgc tacgccaaaa cctaccgaga aaccggcgca aatcagcgtg ataccaaaca
    52081 gcgaaaccgc atgggaaggt gaaacggcaa gggtgatccc cgccgccagg aataacacct
    52141 gtgaggtgat catcaacgta cgtggcttaa aaattcccag cgcaaacggc gtggagaatg
    52201 aaacggtcat gctgacgcca gcggtcaacg ccataatggt ggcgtattct ccgcgctcaa
    52261 aacccatgat ttccatcagc aatactggcg acgtgttgac gaaggtgagg atcaccgaaa
    52321 cgctgagggt ggtgataaca acacggctga ggaaaaaccg gttaagcagt gactcgctat
    52381 tttctcgtgg tttgtcagaa gctgcggggg ccgcagggcg cgtttctttt aaaataaaca
    52441 aagacaacat cagtaccgcg atgcccatcg ttgccatcgt ccagaacaga ctctgccaag
    52501 ggaatttaag cataatcagg tgtccgagca ccggcgctaa caccggaatg atgcaggtaa
    52561 tgccgttgag tagcgacagt acctttgccc gacgtcgatc atccagtgtg tcgcgtaaaa
    52621 tggcgaacgc caccacgtaa caacagcccg cgcccagccc ctgtagaaag cgccctgcaa
    52681 gaaataacgt gctggtttca gccagtgaac agaacaccga ggcgataata aatagcgccg
    52741 cgccgggtat ggcgacaggc tttctccctg aacgatcggc cactttaccg gcaaataaca
    52801 tcgcagctgc catgcccgcc agataaacgg agaacgcaat atgcaactgc gcttcgctgg
    52861 cattgagatc ggcagcgatg cgcggtaaac caacgaggta catatcaatc ccggcgggat
    52921 aaagtaaaac cagggcaaaa ctacaaatca aaaagcggga catgggctaa agcgtcctga
    52981 tagcaaggtg acgcaaagca tacgtggtga aggagaatca ggcgagttgc catttggaca
    53041 taagtgattt cctgacaggc aagaagccag cgaaatggct ggcttcttga agggatcagc
    53101 caaatttagg taacacgtta aagacgttac cgaaccagca taaaattacg gttataccaa
    53161 agagaatgac aatcgccgga ataagattgc cgccccagac cgtgaacatc tgattgggaa
    53221 acttcttgcg agctttgatt gccagcactg cgggaataat gaccgcccag atggtggcgc
    53281 acagtccggc accgccgatc ccgtaaataa agccgttcgg gaagatcaga tacaacaacg
    53341 cgggtggcag gaaggttaac agcacggttt tgaaacgccc gccgtgggag ttatcaatct
    53401 taaacagatc cgccagataa tcgaacaatc ccagcgtgac accaaagaac gaactggcaa
    53461 cagctaagtt ggagaacacc agcaggcaaa actcgataat cccatgctgt ttggtgccga
    53521 ggaacgattt caccagcgaa tcaacgttgc cgcctgagga gattatcgcc ttaaagcttt
    53581 cgcgcggaat attccccatc gtgcaataga gccagaagag ataaatcacc agcgccagca
    53641 gcgaaccaaa gaccacgctt ttgattaact tatctttgcg tttgccatag caaataatca
    53701 ggctgggaat attgccgtgg aaaccaaatg acgccagaca caccggcaaa gccataaaga
    53761 tatacgggaa gtaagacgtt cccgcagtgg tgctggtggc atcgcgcaga atggagtaat
    53821 cgacctggaa gaagaaagaa ccaaacacga tcacaaagga gataatcttc agcccgagga
    53881 acaatgaggt aatacgactg gcggctaacg agcttatcca caatacgctg gcaacgaaaa
    53941 tggctgtgca gatcccgaca atacgcggat tagcgtgata gcccaaattc attgatatcg
    54001 tttcactaat gatcgcacca ttagcagaga tataggcata agtgaggata tagagaacga
    54061 aggcaacggt aataccgctg ataatgttct aggtgttacc gattaaatct ttggtgatgg
    54121 tgttaaaact ggagccgaca ggataattta aatttgcttc taataacaat aacccggaat
    54181 gaagcattga aaaccaggca atgataagga taaaggcacc ccagaaaaac caggcaccgg
    54241 caagatcaac aggtaaagca aacatacctc caccaattac tgtacctgct ataaccataa
    54301 caccccaaaa tgcagagtgc tttttttcag cttgatcagt catgatgcca cctttagagg
    54361 aaggctattt ttgttattga ggatgtaggg taagagagtg gctaacatcc ttatagccac
    54421 tctgtagtat taattaaact tctttcagtt ttgcggtgaa gtgacgcaat actttcggtt
    54481 cgtaggtaaa ggttaaccct ttaatattcg ccgcgttctc tttcacatgt ttgaaggctt
    54541 caataatgaa gtccatatgt gtttgagtat atgttgcgcg cggaatggtt aaacgcagca
    54601 gttcagccgg gcatggcagt tgtttaccgg ttttcggatc gcggcctaac aggaaagagc
    54661 caatttctac cgcacggata ccggcgactt tatacagctc gcaggccagc gcctgtgccg
    54721 ggaactgatc tgccgggata tgcggcagca gtttaccggc atcaacgaat gcagcgtgac
    54781 cgcccgcctg ctggcagaca acgccaatct cttccagacc atcgaccaga tactgcacct
    54841 gcgcgatacg ataagccagc cagtccagat tcatgccgtc atacagacct accgccagac
    54901 gctccatcgc gccgccttcc aggccgccat atgtcgggaa gccttcctgt accacgcaaa
    54961 gggttctgca ctcggtgtac acatcaaaga agctgtcgtc tttcacgcac agcaggccgc
    55021 ccatcggcac cattgcatct ttcttggcgg acatcgccag catatcggca tatttgtagg
    55081 tttcgcgggt gatctgctcg atggtccagt ctttgtattc tgcttcacgc tgcttgatga
    55141 aataggcgtt ttcggcaaag cgtgcggagt ccatgaccac cggaatatcg tatttcttcg
    55201 cgatgctgta catcgctttt aagtttgcca gtgaaaccgg ctgaccacct gcggagttac
    55261 tggtgatggt tgcaacgata tacggcacgt tattcgggcc aacttcttca ataccgcgtt
    55321 ctaatccctc aaggtcaaag ttgcctttaa agtcgtaacg cacgccagta tcgaaggctt
    55381 ctttgatata gacgttacgc acggtacagc cgttaatctg gctatggccc tgcgtggtat
    55441 caaagaaata gttagagaac gccaccattt tgctgcgatc caggcctttt tcctgctcgc
    55501 gttttttaat cagtaccgga atatagattt gttctgcgcc acggccctgg tgagtcggaa
    55561 tggtgtattg ataaccaaag atatttttca ctgactcggc taacgcatag tagctgcggc
    55621 tgccgctgta ggcttcgtcg ccgcgcatca tcgcggcctg catgctctgc gtcaccgccc
    55681 cggtgccgct gtcggtcagt aaatcgataa acacatcttc gctatccagc aggaacgggt
    55741 tcataccgga tttaataatt gcctcttcac ggtaagcgcg agtcgtacgt tttactggct
    55801 caataacacg aatgcggaat ggttcaggga gatgtttaaa gttttccatt acataatcct
    55861 tcatttattt taattacagt gatccctgtg aatattacat ctgcaatatc cattaattcg
    55921 ccagatatat atgcaagcac catcatttac taaaacaagt aaataaatga gagatgagct
    55981 ttgcaaaagg caaaattaag atatagcaaa caaatagatc acattgaatc taatcggttt
    56041 ggctctggtg atggctacag aagggcaaat caagggcggt gatcgacaat cttgttgtca
    56101 atattgaacc attttgaggt cacacatata tgtaagatat tcataatgca cttatcctcg
    56161 aaagacacga ggctacagta taatactcaa gaaatttgtc tattctgaaa ttgattaaat
    56221 gtgaatcgaa tcacaatcgt tcggggagca atattaaatg ctttttttaa gattataaat
    56281 tacgtaagaa ttttaatgtt taaaaaaagg gttgaggcat aacaggagtg ctaacaatga
    56341 agcgaagccg catctgacag tcagaatacg gcttcgtaag cgcagttact taccaataca
    56401 gaagctggag aaaatccgcc caagcaggtc gtctgaagta aactccccgg tgatttcgct
    56461 taagttctgc tgcgccaggc gcaactcttc cgccagcagt tcacccgccc aggcacccaa
    56521 cagttgcgct ttaccttgct gcaaatgctc tgccgcctgt tccagcgcct gtaggtgacg
    56581 acgacgcgcc aggaagccgc cttccatatt ggtgtcaaag cccatgctct gtttgagatg
    56641 gttacgcagc acatccacgc cttcgccagt ccgcgccgaa agacgaatta acgcatgacc
    56701 gttcacttcg ctcatcccca acgtttcgcc ggtgatatcg gctttattgc gcaccacggt
    56761 gatcggcagt ttcgctggca gacgggcgat aaattccggc cagatctctg ccggatcaac
    56821 ggcgtctgtc gtggtgccat cgaccataaa cagcacgcgg tcggcctgtt caatttcctg
    56881 ccacgcgcgc tcgataccaa tacgttccac ttcgtcactg gcttcacgta gcccggcggt
    56941 atcgatgata tgcagcggca ttccgtcaat gtggatatgc tcacgcagca cgtcacgcgt
    57001 ggttccggcg atatcggtta cgattgccgc ttcacggccc gccagcgcgt ttaacaggct
    57061 cgatttaccg gcgttaggac gtccggcaat caccactttc atcccttcgc gcaacaaact
    57121 accctgacgt gcttcagcac gcactgcatc aagatcggca ataacgtcat tgagctgggc
    57181 ttcaattttg ccgtcggaga ggaaatcgat ctcttcatcc gggaaatcaa ttgccgcttc
    57241 gacgtaaatg cgcaagtggg tgagggcttc caccagatgg ttgacccgtg cggagaatgc
    57301 gccttgcagc gagtttagcg ccgaacgggc cgcctgttcc gagctggcgt cgataagatc
    57361 agcaatcgct tcggcctggg ctaagtcgag tttatcgttc agaaatgcgc gttcggaaaa
    57421 ctcaccaggg cgggcaatcc gcaggccggg aatggtcaga atgcgtttta acagcaggtc
    57481 gaggatcacc ggaccgccat gaccttgcag ttccagcaca tcttcgccgg tgaacgagtt
    57541 cgggccaggg aaccatagcg caatcccctg atcgagcacg ctgccgtcgg cgtctttaaa
    57601 cggaagataa tcggcgtagc gcggctttgg cagtttaccc agcacggttt cggcaacctc
    57661 acgggctttg aggccggaga tgcgcaggat gccaacgccg ccacgtcccg gaggcgtggc
    57721 ctgggctacg atagtgtcat tatcgctcat gatgttcctg ttgctttgtg tggcggatgc
    57781 gcggtgctta tccgccctac gaaaagaaaa aaggcggtca actgaccgcc cttattttag
    57841 cgaaaactca ccgaatcagg attttttctt ctcgcggcta tgcaggccac gtttttccag
    57901 accacggtaa atcagctgct gctgaataat ggttaccagg ttgctgacga tatagtacag
    57961 caccagacct gacgggaacc acaggaagaa cacggtgaag atgaccggca taaaggtcat
    58021 gatcttctgc tgcatcgggt cggtcacggt ggtcggcgac atcttctgaa tgaagaacat
    58081 cgttacgccc atcaggatcg gcaggatgta gtacgggtcc tgtgcagaca ggtcgtggat
    58141 ccacagtgca aacggtgcct gacgcagttc aacggaaccc atcagcatgt agtacaacgc
    58201 caggaagatt ggcatctgga tcagcagcgg gaagcagccg cccagcgggt taaccttctc
    58261 agctttgtac agcgccatca tttcctgact gatacgctgt ttgtcatcgc ccagacgctc
    58321 acgcatagcc tgaatcttcg gctgcagcat acgcatcttc gccatggagg tgtactgcgc
    58381 tttggtcagc gggtacatga tgccacgaac gataaaggtg atgatgataa tggagaagcc
    58441 ccagttaccc acaaagctat ggatccattt cagcagtttg aacagcggct gagaaatgaa
    58501 ccacaaccaa ccgtaatcaa cggtcagatc caggtgcgga gcaacagctg ccattttgtc
    58561 ctggatttcc gggccaaccc acagggtgct gttcatcgcg ccagtctgac caggctgaac
    58621 cagtaccggc tgagatttat agccgatagc ggcgatgccg ttacccagat tagcggtata
    58681 gaagttgttg gtaccgtcgt tatgcgggat ccacgccgtc gcgaaatact gttgcagcat
    58741 cgccacccaa ccacctttcg aagagatgtt caggttttcg ttatcggcaa tggtatcgaa
    58801 cttgtatttc tcgtacttct cgtcaggcgt ggagtacgcc gcgccacgga aggtgtgcag
    58861 tgcgaagttg ctgcttccgg tatcgagatg cggtggcaga gtgatggatt gcttcaactg
    58921 accaaaggtg gagatttcca gcggtttctc gccagcgttc tgcacgttgt agttgacgtt
    58981 gacagcgtaa tcaccacgtt tcaggacaaa cgttttggta aacgtgttgc ctgccgcgtc
    59041 ggtatacgtc atcggcacct gcagttcgtt ttgaccttca gccagcacat aagcgtcctt
    59101 ttcaacgtta tacagcggac gcgggccgtt agccgggtta tccgggccat cacgaccggt
    59161 cagaccgctc tgtgcctgat aaataaactg cggtgaagtt tccagcaact ggaacggctg
    59221 ggtagagttc agctctttcg ggtaagcagg cagcagagct tgctcaacat caccaccacg
    59281 ggtgttgatg gtcagatcaa gcacgtcggt cttaaccgag atcagtttcc cctggccact
    59341 ggccggtacg ccctggtcgg cggcgctacc cgctgcggtg gtcgttgtct gcgtggtctg
    59401 ttgggcctga ggttgcgggt ttttatcctg ctcccaggct tgccagatca tgaaagacac
    59461 gaacagcaaa gcgatgacta aaagattgcg ttgcgaatcc atcgttagtg ttctctggta
    59521 tcaaatggtc cgggcgggac gggatcgtca ccaccagggt gtaaagggtg gcattttaat
    59581 acgcgtttca ccgtcaacca actgcctttt atcactccaa acctgcgcaa tgcctcaatt
    59641 ccgtagcttg aacaggttgg agtgaaacga caatgcggcc cgagtagcgg actaatcagg
    59701 cgttgataga cccgaatgag ggctatcagg acccgcgagc caggcgacag tggcggcgcc
    59761 ataatttttc caacgcttcc gagagagcac ggttatcgag gtcggcaacc ccttttttcg
    59821 ccaccaccac gaaatccata gccgggagtt catgttggcg cagacggaag ctttcacgcg
    59881 tcagacgttt aatccgattg cgttcatggg cgcgtcgaac gtttttcttg gcgactgtaa
    59941 gaccgatacg gggatgcccc agcgaattca ggcggccgag aatggtaatt tgcggcgtgc
    60001 cagcccgttg tggctgctgg aagacgaatg tgaattgact gggagttaac aagcgtaact
    60061 ccctgggaaa tgcgagctta accactcagg ggttagcttt attacttaga aacggtcaga
    60121 cgagcgcggc ctttagcacg acgacgtgcc agaacctgac gaccattttt agtagccata
    60181 cgagcacgga agccgtgaga acggttgcgc ttcagtacag acggttgaaa agtgcgtttc
    60241 atggcgattt ctacctaaac ttgaataaat tcaatggctt tattggatat ccgccgaaaa
    60301 atgaaacgat ggacaccgaa gccatgggtg attaaagagg ccggattgta ataattgtac
    60361 actccggagt caattctctt tccttattta ccgcgctttt ccgcaccttt tcgcggggaa
    60421 aacgtacggc ctcacaccag tgaaaaccag catggcgcgc cgggtggagg attatacggg
    60481 ctgacgggta aagcgcaagg atcgtcctgg atctttatta gatcgattaa gccaattttt
    60541 gtctatggtc attaaatttt ccaatatgcg gcgtaaatcg tgcccggccc gcggcaggat
    60601 cgtttacact tagcgagttc tggaaagtcc tgtggataaa tcgggaaaat ctgtgagaaa
    60661 cagaagatct cttgcgcagt ttaggctatg atccgcggtc ccgatcgttt tgcaggatct
    60721 tgatcgggca tataaccgca gacagcggtt cgtgcgtcac cctcatgcag ggtcttttcg
    60781 acgtacgtca acaatcatga atgtttcagc cttagtcatt atcgactttt gttcgagtgg
    60841 agtccgccgt gtcactttcg ctttggcagc agtgtcttgc ccgattgcag gatgagttac
    60901 cagccacaga attcagtatg tggatacgcc cattgcaggc ggaactgagc gataacacgc
    60961 tggccctgta cgcgccaaac cgttttgtcc tcgattgggt acgggacaag taccttaata
    61021 atatcaatgg actgctaacc agtttctgcg gagcggatgc cccacagctg cgttttgaag
    61081 tcggcaccaa accggtgacg caaacgccac aagcggcagt gacgagcaac gtcgcggccc
    61141 ctgcacaggt ggcgcaaacg cagccgcaac gtgctgcgcc ttctacgcgc tcgggttggg
    61201 ataacgtccc ggctccggca gaaccgacct atcgttctaa cgtaaacgtc aaacacacgt
    61261 ttgataactt cgttgaaggt aaatctaacc aactggcgcg cgcggcggct cgccaggtgg
    61321 cagataaccc tggcggtgct tataacccgt tgttccttta tggcggcacg ggtttgggta
    61381 aaactcacct gctgcatgcg gtgggtaacg gcattatggc gcgcaagccg aatgccaaag
    61441 tggtttatat gcactccgag cgctttgttc aggacatggt taaagccctg caaaacaacg
    61501 cgatcgaaga gtttaaacgc tactaccgtt ccgtagatgc actgctgatc gacgatattc
    61561 agttttttgc taataaagaa cgatctcagg aagagttttt ccacaccttc aacgccctgc
    61621 tggaaggtaa tcaacagatc attctcacct ccgatcgcta tccgaaagag atcaacggcg
    61681 ttgaggatcg tttgaaatcc cgcttcggct ggggactgac tgtggcgatc gaaccgccag
    61741 agctggaaac ccgcgtggcg atcctgatga aaaaggccga cgaaaacgac attcgtttgc
    61801 cgggcgaagt ggcgttcttt atcgccaagc gtctacgatc taacgtacgt gagctggaag
    61861 gcgcgctgaa ccgcgttatt gctaacgcca actttaccgg acgtgcgatc accatcgact
    61921 tcgtgcgtga ggcgctgcgc gacttgctgg cattgcagga aaaactggtc accatcgaca
    61981 atattcagaa gacggtggcg gagtactaca agatcaaagt cgcggatctc ctttccaagc
    62041 gtcgatcccg ctcggtggcg cgtccgcgcc agatggcgat ggcgctggca aaagaactga
    62101 ctaaccacag tctgccggag attggcgatg cgtttggtgg ccgtgaccac acgacggtgc
    62161 ttcatgcctg ccgtaagatc gagcagctgc gtgaagagag ccacgatatc aaagaagatt
    62221 tttccaattt aatcagaaca ttgtcatcgt aaacctatga aatttaccgt agaacgtgag
    62281 catttattaa aaccgctaca acaggtgagc gggccgttag gtggtcgtcc tacgctaccg
    62341 attctcggta atctgctgtt acaggttgct gacggtacgt tgtcgctgac cggtactgat
    62401 ctcgagatgg aaatggtggc acgcgttgcg ctggttcagc cacacgaacc aggggcgacg
    62461 accgttccag cgcgcaaatt ctttgatatc tgccgtggtc tgcctgaagg cgcagaaatt
    62521 gccgtgcagc tggaaggtga gcggatgctg gtgcgctccg ggcgtagccg tttttcgctg
    62581 tctaccctgc cagcggcgga tttcccgaat cttgatgact ggcagagcga agtcgaattt
    62641 accctgccgc aggcgacgat gaagcgtctg attgaagcga cccagttttc tatggcgcat
    62701 caggacgttc gctattactt aaacggtatg ctgttcgaaa ctgaaggtga agaactgcgc
    62761 accgtggcga ccgatggtca ccgtctggcg gtctgttcaa tgccaattgg tcaatctttg
    62821 ccaagccatt cggtgatcgt gccgcgtaaa ggcgtgattg aactgatgcg tatgctcgac
    62881 ggcggcgaca atccgctgcg cgtgcagatt ggcagcaaca acattcgcgc ccacgttggc
    62941 gactttatct tcacctcgaa actggtggat ggtcgcttcc cggattaccg tcgtgttctg
    63001 ccgaagaatc cggacaaaca tctcgaagca gggtgcgatc tgctcaagca ggcgtttgcc
    63061 cgtgcggcga tcctctctaa cgagaaattc cgcggcgtgc gtctgtatgt cagcgaaaat
    63121 cagctgaaaa tcaccgccaa taacccggaa caggaagaag cggaagagat cctcgacgtt
    63181 acctatagcg gtgcggagat ggaaatcggc ttcaatgtca gctatgtgct ggatgttctg
    63241 aacgcgctga aatgcgaaaa cgtccgcatg atgctgaccg attcggtttc cagcgtgcag
    63301 attgaagatg ccgcatcaca gtcggctgcc tatgttgtca tgccaatgag actgtaatgt
    63361 ccctcacccg cttgttgatc cgcgatttcc gcaatatcga aaccgcggat ctcgctttat
    63421 ctcccggctt taactttctg gtaggtgcca acggtagtgg caaaaccagc gtgctggaag
    63481 ccatctatac gctcggccat ggtcgggcgt ttcgcagttt gcagattggt cgcgtcattc
    63541 gccacgagca ggaggcgttt gttctccacg ggcgattaca gggcgaagag cgcgagacag
    63601 cgattggctt aaccaaagac aaacagggcg acagcaaagt ccgcatcgac ggtacagacg
    63661 ggcataaggt cgcggaactg gcgcacctga tgccaatgca gctgataacg ccagaagggt
    63721 ttactttact caacggcggc cccaaataca gaagagcatt cctcgactgg ggatgctttc
    63781 acaacgaacc cggatttttc accgcctgga gcaatctcaa gcgattgctc aagcagcgca
    63841 atgcggcgct gcgccaggtg actcgttacg aacagctacg cccgtgggat aaagaactga
    63901 tcccgctggc ggagcaaatc agcacctggc gcgcggagta tagcgccggt atcgcagccg
    63961 atatggctga tacctgtaag caatttctcc ctgagttttc tctgactttc tctttccagc
    64021 gcggctggga gaaagagaca gagtacgctg aggtgctgga acgtaatttt gaacgcgatc
    64081 gccagctaac ctacaccgcg cacggcccgc ataaagcgga cttacgcatt cgcgccgacg
    64141 gtgcgccggt ggaagatacc ttatcgcgtg ggcagcttaa gctgttgatg tgcgccttac
    64201 gtctggcgca aggagagttc ctcacccgtg aaagcgggcg gcggtgtctc tacctgatag
    64261 atgattttgc ctctgagctt gatgatgagc gtcgcgggct gcttgccagc cgcttaaaag
    64321 cgacgcaatc acaggtcttt gtcagcgcga tcagtgctga acacgttata gacatgtcgg
    64381 acgaaaattc gaagatgttt accgtggaaa agggtaaaat aacggattaa cccaagtata
    64441 aatgagcgag aaacgttgat gtcgaattct tatgactcct ccagtatcaa agtcctgaaa
    64501 gggctggatg cggtgcgtaa gcgcccgggt atgtatatcg gcgacacgga tgacggcacc
    64561 ggtctgcacc acatggtatt cgaggtggta gataacgcta tcgacgaagc gctcgcgggt
    64621 cactgtaaag aaattatcgt caccattcac gccgataact ctgtctctgt acaggatgac
    64681 gggcgcggca ttccgaccgg tattcacccg gaagagggcg tatcggcggc ggaagtgatc
    64741 atgaccgttc tgcacgcagg cggtaaattt gacgataact cctataaagt gtccggcggt
    64801 ctgcacggcg ttggtgtttc ggtagtaaac gccctgtcgc aaaaactgga gctggttatc
    64861 cagcgcgagg gtaaaattca ccgtcagatc tacgaacacg gtgtaccgca ggccccgctg
    64921 gcggttaccg gcgagactga aaaaaccggc accatggtgc gtttctggcc aagccttgaa
    64981 accttcacca atgtgaccga gttcgaatat gacattctgg cgaaacgtct gcgtgagttg
    65041 tcgttcctca actccggcgt ttccattcgt ctgcgcgata agcgcgacgg caaagaagac
    65101 cacttccact atgaaggcgg catcaaggcg ttcgttgaat atctgaacaa gaacaaaacg
    65161 ccgatccacc cgaatatctt ctacttctcc actgaaaaag acggtattgg cgtcgaagtg
    65221 gcgttgcagt ggaacgatgg cttccaggaa aacatctact gctttaccaa caacattccg
    65281 cagcgtgacg gcggtactca cctggcaggc ttccgtgcgg cgatgacccg taccctgaac
    65341 gcctacatgg acaaagaagg ctacagcaaa aaagccaaag tcagcgccac cggtgacgat
    65401 gcgcgtgaag gcctgattgc ggtcgtttcc gtgaaagtgc cggacccgaa attctcctcc
    65461 cagaccaaag acaaactggt ttcttctgag gtgaaatcgg cggttgaaca gcagatgaac
    65521 gaactgctgg cggaatacct gctggaaaac ccaaccgacg cgaaaatcgt ggttggcaaa
    65581 attatcgatg ctgcccgtgc ccgtgaagct gcgcgtcgcg cgcgtgaaat gacccgccgt
    65641 aaaggtgcgc tcgacttagc gggcctgccg ggcaaactgg cagactgcca ggaacgcgat
    65701 ccggcgctct ctgaactgta cctggtggaa ggggactccg cgggcggctc tgcgaagcag
    65761 gggcgtaacc gcaagaacca ggcgattctg ccgctgaagg gtaaaatcct caacgttgag
    65821 aaagcgcgct tcgataagat gctctcttct caggaagtgg cgacgctgat caccgcgctt
    65881 ggctgtggta tcggtcgtga cgagtacaac ccggacaaac tgcgttatca caacatcatc
    65941 atcatgaccg atgcggacgt cgacggctcg cacattcgta cgctgctgtt gaccttcttc
    66001 tatcgtcaga tgccggaaat cgttgaacgc ggtcacgtct acatcgctca gccgccgctg
    66061 tacaaagtga agaaaggcaa gcaggaacag tacattaaag acgacgaagc gatggatcag
    66121 taccagatct ctatcgcgct ggatggcgca acgctgcaca ccaacgccag tgcaccggcg
    66181 ctggctggcg aagcgttaga gaaactggtg tctgagtaca acgcgacgca gaaaatgatc
    66241 aaccgcatgg agcgtcgtta tccgaaagca atgctgaaag agcttatcta tcagccgacg
    66301 ttgacggaaa ccgacctttc tgacgagcaa accgttaccc gctgggtgaa cgcgctggtc
    66361 agcgaactga acgataaaga acagcacggc agccagtgga agtttgatgt ccataccaat
    66421 gccgaacaaa acctgttcga gccgattgtt cgcgtgcgta cccacggtgt ggatactgac
    66481 tatccgctgg atcacgaatt tatcaccggc ggcgaatatc gtcgtatctg cacgctgggt
    66541 gagaaactgc gtggcttgct ggaagaagat gcatttatcg aacgtggcga acgtcgtcag
    66601 ccggtagcca gcttcgagca ggcgctggac tggctggtga aagagtcccg tcgcggcctc
    66661 tccatccagc gttataaagg tctgggcgag atgaacccgg aacagctgtg ggaaaccacc
    66721 atggatccgg aaagccgtcg tatgctgcgc gttaccgtta aagatgcgat tgctgccgac
    66781 cagttgttca ctacgcttat gggcgacgcc gttgaaccgc gccgtgcgtt tatcgaagag
    66841 aacgccctga aagcggcaaa tatcgatatt taatggcttt ttgttgctga taaaatgact
    66901 tagtcgtcag tccgggcgga gatcatctcc gcccttttta tttctgcaat ccgcaattat
    66961 catcccgcag atctatactt tttgggtcaa cacgtaacgc tttacaggtg attatcatgg
    67021 ggctttttga tgacgttgtc ggtgcctttc tgaaaggcga tgcggggaaa tatcaggcca
    67081 ttttgagctg ggttgaggag cagggcggca ttcaggtgct gctggaaaaa ctgcaaagtg
    67141 gcggtttagg tgacattctc tcaacctggc tgagtaatca acagggcaac caaccagtta
    67201 gcggcgagca actggaatca gcgctcggca caaatgcggt gtccgatctc gggcaaaaac
    67261 tgggcgtgga taccagtaca gcttccagtt tactggcaga acaattgccg aagattattg
    67321 atgcactctc accgcaaggt gaagtgtcag cacaagccaa taacgatctg ctttccgcag
    67381 gcatggaact gctgaaaggg aaactcttcc gctaagcaaa tggggagcat gcaacccgca
    67441 ttgcatcccc cttttccctc gggtgttgta ctgatttttg agcggaatcg cgttagcatg
    67501 ggtcaggaac caatctacct ggggaactca tggctatcaa actcattgct atcgatatgg
    67561 atggcaccct tctgctgccc gatcacacca tttcacccgc cgttaaaaat gcgattgccg
    67621 cagctcgcgc ccgtggcgtg aatgtcgtgc tgacgacggg tcgcccttat gcaggtgttc
    67681 acaattacct gaaagagctg catatggaac agccgggcga ctactgcatt acttataacg
    67741 gcgcgctggt acagaaggcc gctgatggta gcaccgtggc gcaaaccgct ctcagctatg
    67801 acgactaccg tttcctggaa aaactctctc gcgaagtcgg ttctcatttc cacgccctgg
    67861 accgcaccac gctgtacacc gccaaccgag atatcagcta ctacacggtg catgaatctt
    67921 tcgttgccac cattccactg gtgttctgcg aagcggagaa aatggacccc aatacccagt
    67981 tcctgaaagt gatgatgatt gatgaacccg ccatcctcga ccaggccatt gcgcgtattc
    68041 cgcaggaagt gaaagaaaaa tacaccgtgc tgaaaagtgc gccgtacttc ctcgaaatcc
    68101 tcgataaacg cgttaacaaa ggtacgggtg tgaaatcact ggccgacgtg ttaggtatta
    68161 aaccggaaga aatcatggcg attggcgatc aggaaaacga catcgcaatg attgaatacg
    68221 caggtgttgg tgtggcaatg gataacgcta ttccttcggt gaaagaagtg gcgaactttg
    68281 tcaccaaatc caacctggaa gatggcgtgg cgtttgctat tgagaagtat gtgctgaatt
    68341 aatcagtggg cgggcaaact gcataaatgc gtttgcccgc agcgattaac aatttctggt
    68401 cgtgcataaa atcaggaact ggagtggtga cgttactata tccagcgtaa gagtgactgg
    68461 tagcagcgca ttagcgaggc cgcgcggaac gccatcagtt gctttgtact tataaaatcc
    68521 caccgtaaag ggttgatgga aaaccatcac gtttgagtaa tccgcttttt tatttttctg
    68581 gtggatagag cctttcaggc cgtcaagtgc ccaggtacaa cgctgggtat tacaaataaa
    68641 accatattgt gtcgccagtg ctttatcttc ctcattatcc cagtcgtaat agagcgttat
    68701 tgaaccagaa aaattagtat cactgctgct taaaataaat ttcgcgttat cagaaacggt
    68761 gattctttca ccatggattg ccggatcgtt cagcatttta actacctcat cgccgccggt
    68821 ggtcaacaga tagtccaatg attcaccaac aaaaacatag cctttcgtac cgttactatc
    68881 ttctgccagc gataaaccct taatcgtgtc tgaccgccat tgctcggcat gtggacggct
    68941 tgcagtattg gaaaccaatc cccaggtgat acatccggtc aacgcaaatg ccaaagaaca
    69001 caaactggca gccttgaaaa atcccttaag attcaacttc atgattaatc ctttaaacaa
    69061 gagtactaag ccgttgaaag catactcttc tttcccccca gtttagcgcc tgatctccct
    69121 tttcagattt ttgcccgacg tagaacattt tcttatggat tgttcttttt gtgatctaaa
    69181 ttgtagtaca acaatataat tttgtactac attacgcaca cggcaaacgc gaacgtcatc
    69241 acgctggtac tacaaagttg ctgcgttatg catcgatctg ggtaaagtag agaagaacat
    69301 acagagcaca aggactctcc atgactctca ataaaaccga tcgcattgtc attacgctgg
    69361 gtaaacagat cgttcacggc aaatacgtac caggctctcc acttccggct gaggcggagc
    69421 tctgtgaaga gtttgcaacc tcgcgcaaca tcatccgtga ggtgttccgt tcgctgatgg
    69481 cgaagcggct gattgaaatg aaacgttatc gcggggcgtt tgtggcaccg cgtaatcagt
    69541 ggaattacct cgatactgac gtgctgcaat gggtgctgga aaacgactac gacccacggc
    69601 ttatcagtgc aatgagcgaa gtgcgaaatc tggtggaacc ggcgattgcc cgttgggcgg
    69661 cagagcgcgc gacttccagc gatctggcgc agattgaatc agcgctgaac gagatgattg
    69721 ccaacaatca ggaccgcgaa gcgtttaacg aagcggatat tcgctaccac gaggcggtgc
    69781 tgcagtcggt gcataacccg gtgttacagc aacttagcat tgcgatcagt tcgctgcagc
    69841 gggcggtttt tgaacgaacc tggatgggcg atgaggccaa catgccgcaa acgctccagg
    69901 aacataaggc gctgtttgat gcgatacggc atcaggacgg cgatgcggca gagcaggcgg
    69961 cgcttaccat gatcgccagc tcgacacgaa ggttaaagga aatcacatga cagctcgcta
    70021 catcgcaatt gactggggat cgaccaatct gcgcgcctgg cttta
//
LOCUS       DAMZFT010000010        65535 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.010, whole genome shotgun sequence.
ACCESSION   DAMZFT010000010 DAMZFT010000000
VERSION     DAMZFT010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 65535)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 65535)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..65535
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.010"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(<1..506)
                     /gene="yeiB"
                     /locus_tag="PN977_000757"
     CDS             complement(<1..506)
                     /gene="yeiB"
                     /locus_tag="PN977_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF418 domain-containing protein"
                     /protein_id="HDI6040821.1"
                     /translation="MERNVTLDFVRGVAILGILLLNISAFGLPKAAYLNPAWYGAITS
                     QDAWTWAFLDLIGQVKFLTLFALLFGAGLQMLLPRGRRWIQSRLTLLVLLGFIHGLLF
                     WDGDILLAYGLVGLICWRLVRDAPSVKSLFNTGVMLYLVGLGVLLLLGLISDSQTSRA
                     WTPDASAI"
     gene            complement(523..1191)
                     /gene="folE"
                     /locus_tag="PN977_000758"
     CDS             complement(523..1191)
                     /gene="folE"
                     /locus_tag="PN977_000758"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HDI6040822.1"
                     /translation="MPSLSKEAALVHEALVARGLETPLRPPVHEMDNETRKSLIAGHM
                     TEIMQLLNLDLADDSLMETPHRIAKMYVDEIFSGLDYANFPKITLIENKMKVDEMVTV
                     RDITLTSTCEHHFVTIDGKATVAYIPKDSVIGLSKINRIVQFFAQRPQVQERLTQQIL
                     IALQTLLGTNNVAVSIDAVHYCVKARGIRDATSATTTTSLGGLFKSSQNTRHEFLRAV
                     RHHN"
     gene            1448..2284
                     /gene="yeiG"
                     /locus_tag="PN977_000759"
     CDS             1448..2284
                     /gene="yeiG"
                     /locus_tag="PN977_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HDI6040823.1"
                     /translation="MELLEEHLCFEGWQQRWRHDSSTLNCPMTFSIFLPPPRDHTPPP
                     VLYWLSGLTCNDENFTTKAGAQRVAAELGIVLVMPDTSPRGEQVANDDGYDLGQGAGF
                     YLNATQPPWATHYRMYDYLRDELPALVQSQFNVSDRCAISGHSMGGHGALIMALKNPG
                     KYTSVSAFAPIVNPCSIPWGIKAFSRYLGEDKNAWLEWDSCALMYASNAQDAIPTLID
                     QGDNDQFLADQLQPAVLAEAARQKAWPMTLRIQPGYDHSYYFIASFIEDHLRFHAQYL
                     LK"
     gene            complement(2316..4307)
                     /gene="cirA"
                     /locus_tag="PN977_000760"
     CDS             complement(2316..4307)
                     /gene="cirA"
                     /locus_tag="PN977_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catecholate siderophore receptor CirA"
                     /protein_id="HDI6040824.1"
                     /translation="MFRLNPFVRVGLCLSAISCAWPVLAVDDDGETMVVTASSVEQNL
                     KDAPASISVITQEDLQRKPVQNLKDVLKEVPGVQLTNEGDNRKGVSIRGLDSSYTLIL
                     VDGKRVNSRNAVFRHNDFDLNWIPVDSIERIEVVRGPMSSLYGSDALGGVVNIITKKI
                     GQKWSGTVTVDTTVQEHRDRGDTYNGQFFTSGPLIDGVLGMKAYGSLAKREKDDPQNS
                     TTTDTGETPRIEGFSSRDGNVEFAWTPNQNHDFTAGYGFDRQDRDSDSLDKNRLERQN
                     YSVSHNGRWDYGTSELKYYGEKVENKNPGYSSPITSESNTVDGKYTLPLTAINQFLTV
                     GGEWRHDKLSDAVNLTGGTSSKTSASQYALFVEDEWRIFEPLALTTGVRMDDHETYGE
                     HWSPRAYLVYNATDTVTVKGGWATAFKAPSLLQLSPDWTSNSCRGACKIVGSPDLKPE
                     TSESWELGLYYMGEEGWLEGVESSVTVFRNDVKDRISISRTSDVNAAPGYQNFVGFET
                     GANGRRIPVFSYYNVNKARIQGVETELKIPFNDEWKLSINYTYNDGRDVSNGENKPLS
                     DLPFHTANGTLDWKPLALEDWSFYVSGHYTGQKRADSATAKTPGGYTIWNTGAAWQVT
                     KDVKLCAGVLNLGDKDLSRDDYSYNEDGRRYFMAVDYRF"
     gene            complement(4599..6068)
                     /gene="lysP"
                     /locus_tag="PN977_000761"
     CDS             complement(4599..6068)
                     /gene="lysP"
                     /locus_tag="PN977_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-specific permease"
                     /protein_id="HDI6040825.1"
                     /translation="MGSETKTTEAPGLRRELKARHLTMIAIGGSIGTGLFVASGATIS
                     QAGPGGALLSYMLIGLMVYFLMTSLGELAAYMPVSGSFATYGQNYVEEGFGFALGWNY
                     WYNWAVTIAVDLVAAQLVMSWWFPDTPGWIWSALFLGVIFLLNYISVRGFGEAEYWFS
                     LIKVTTVIVFIIVGVLMIIGIFKGAQPAGWSNWTIGEAPFAGGFAAMIGVAMIVGFSF
                     QGTELIGIAAGESEDPAKNIPRAVRQVFWRILLFYVFAILIISLIIPYTDPSLLRNDV
                     KDISVSPFTLVFQHAGLLSAAAVMNAVILTAVLSAGNSGMYASTRMLYTLACDGKAPR
                     IFAKLSRGGVPRNALYATTVIAGLCFLTSMFGNQTVYLWLLNTSGMTGFIAWLGIAIS
                     HYRFRRGYVLQGHDINDLPYRSGFFPLGPIFAFILCLIITLGQNYEAFLKDTIDWGGV
                     AATYIGIPLFLIIWFGYKLIKGTHFVRYSEMKFPQNDKK"
     gene            complement(6273..7154)
                     /locus_tag="PN977_000762"
     CDS             complement(6273..7154)
                     /locus_tag="PN977_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDI6040826.1"
                     /translation="MHITLRQLEVFAEVLKSGSTTQASVMLALSQSAVSAALTDLEGQ
                     LGVQLFDRVGKRLVVNEHGRLLYPRALALLEQAVEIEQLFREDNGAIRIYASSTIGNY
                     ILPAVIARYRHDYPQLPIELSVGNSQDVMQAVLDFRVDIGFIEGPCHSTEIISEPWLE
                     DELVVFAAPTSPLARGPVTLEQLAAAPWILRERGSGTREIVDYLLLSHLPKFEMAMEL
                     GNSEAIKHAVRHGLGISCLSRRVIEDQLQAGTLSEVAVPLPRLMRTLWRIHHRQKHLS
                     NALRRFLDYCDPANVPR"
     gene            7253..8302
                     /locus_tag="PN977_000763"
     CDS             7253..8302
                     /locus_tag="PN977_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family protein"
                     /protein_id="HDI6040827.1"
                     /translation="MTNITLQKQHRTLWHFIPGLALSAVITGVALWGGSIPAVAGAGF
                     SALTLAILLGMVLGNTIYPHIWKSCDGGVLFAKQYLLRLGIILYGFRLTFSQIADVGI
                     SGIIIDVLTLSSTFLLACFLGQKVFGLDKHTSWLIGAGSSICGAAAVLATEPVVKAEA
                     SKVTVAVATVVIFGTVAIFLYPAIYPLMSQWFSPETFGIYIGSTVHEVAQVVAAGHAI
                     SPDAENAAVISKMLRVMMLAPFLILLAARVKQLSGANSGEKSKITIPWFAILFIVVAI
                     FNSFHLLPQSVVNMLVTLDTFLLAMAMAALGLTTHVSALKKAGAKPLLMALVLFAWLI
                     VGGGAINYVIQSVIA"
     gene            8376..9233
                     /gene="nfo"
                     /locus_tag="PN977_000764"
     CDS             8376..9233
                     /gene="nfo"
                     /locus_tag="PN977_000764"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HDI6040828.1"
                     /translation="MKYIGAHVSAAGGLANAAIRAAEIDATAFALFTKNQRQWRAAPL
                     TTQTIDEFKAACEKYHYTSAQILPHDSYLINLGHPVTEALEKSRDAFIDEMQRCEQLG
                     LSLLNFHPGSHLMQISEEDCLARIAESINIALDKTHGVTAVIENTAGQGSNLGFKFEH
                     LAAIIDGVEDKSRVGVCIDTCHAFAAGYDLRTSAECEKTFADFARIVGFKYLRGMHLN
                     DAKSTFGSRVDRHHSLGEGNIGHDAFRWIMQDDRFDGIPLILETINPDIWAEEIAWLK
                     AQQTEKAVA"
     gene            9236..10324
                     /locus_tag="PN977_000765"
     CDS             9236..10324
                     /locus_tag="PN977_000765"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HDI6040829.1"
                     /translation="MNNREKEILAILRRNPLIQQNEIADMLQISRSRVAAHIMDLMRK
                     GRIKGKGYILTEQEYCVVVGTINMDIRGMADIRYPQAASHPGTIHCSAGGVGRNIAHN
                     LALLGRDVHLLSVIGDDFYGEMLLEETRRAGVNVSGCVRLHGQSTSTYLAIANRDDQT
                     VLAINDTHLLDQLTPQLLNGSRDLLRHAGVVLADCNLTAEALEWVFTLADEIPVFVDT
                     VSEFKAGKIKHWLAHIHTLKPTLSELEILWGQPITRDADRNAAVNALHQQGVQQLFVY
                     LPDESVYCSQKDGEQFLLTAPAHTTVDSFGADDGFMAGLVYSFLEGSSFRDSARFAMA
                     CAAISRASGSLNNPTLSADNALSLVPMV"
     gene            complement(10380..11630)
                     /locus_tag="PN977_000766"
     CDS             complement(10380..11630)
                     /locus_tag="PN977_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HDI6040830.1"
                     /translation="MDVMRSVLGMVVLLAIAFLLSVNKKRISLRTVGAALVLQVVIGG
                     IMLWLPPGRWVAEKVAFGVHKVMAYSDAGSAFIFGSLVGPKMDTLFDGAGFIFGFRVL
                     PAIIFVTALVSILYYIGVMGILIRILGGIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRLNRNELFTAICSGMASIAGSTMIGYAALGVPVEYLLAASLMAIPGGILFAR
                     LLSPATESSQVSFNNLSFTETPPKSIIEAAATGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGVGGWFGFEQASLESILGYLLAPLAWVMGVDWSDANLAGSLIGQKLAINEFVA
                     YLNFSPYLQTAGTLDAKTVAIISFALCGFANFGSIGVVVGAFSAVAPHRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(11730..12671)
                     /gene="rihB"
                     /locus_tag="PN977_000767"
     CDS             complement(11730..12671)
                     /gene="rihB"
                     /locus_tag="PN977_000767"
                     /EC_number="3.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosylpyrimidine nucleosidase"
                     /protein_id="HDI6040831.1"
                     /translation="MEKRKIILDCDPGHDDAIAMMMAAKHPAIDLLGITIVAGNQTLD
                     KTLINGLNVCQKLEINVPVYAGMPQPIMRQQIVADNIHGETGLDGPVFEPLTRQAEST
                     HAVKYIIDTLMASDGDITLVPVGPLSNIAVAMRMQPAILPKIREIVLMGGAYGTGNFT
                     PSAEFNIFADPEAARVVFTSGVPLVMMGLDLTNQTVCTPDVIARMERAGGPAGELFSD
                     IMNFTLKTQFENYGLAGGPVHDATCIGYLINPDGIKTQEMYVEVDVNSGPCYGRTVCD
                     ELGVLGKPANTKVGITIDTDWFWGLVEECVRGYIKTH"
     gene            12801..13499
                     /gene="yeiL"
                     /locus_tag="PN977_000768"
     CDS             12801..13499
                     /gene="yeiL"
                     /locus_tag="PN977_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311082.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator YeiL"
                     /protein_id="HDI6040832.1"
                     /translation="MKEIHNNDLKQQLMSESAFKDCFSTDVSADARLFHFLARDYIVQ
                     EGQQPSWLFYLTRGRARLYATLANGRVSLIDFFAAPCFIGEIELIDKDHEPRAVQAIE
                     ECWCLALPMKHYRPLLLNDTLFLRKLCVSLSHKNYRNIVSLTQNQSFPLVNRLAAFIL
                     LSQEGDLYHEKHTQAAEYLGVSYRHLLYVLAQFIHDGLLIKSKKGYLIKNRKQLSGLA
                     LEMDPENKFSGMMQ"
     gene            complement(13567..14817)
                     /locus_tag="PN977_000769"
     CDS             complement(13567..14817)
                     /locus_tag="PN977_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HDI6040833.1"
                     /translation="MDIMRSVVGMVVLLAIAFLLSVNKKSISLRTVGAALLLQIAIGG
                     IMLYFPPGKWAVEQAALGVHKVMSYSDAGSAFIFGSLVGPKMDVLFDGAGFIFAFRVL
                     PAIIFVTALISLLYYIGVMGLLIRILGSIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRMNRNELFTAICSGMASIAGSMMIGYAGMGVPIDYLLAASLMAIPGGILFAR
                     ILSPATEPSQVTFENLSFSETPPKSFIEAAASGAMTGLKIAAGVATVVMVFVAIIALI
                     NGIIGGIGGWFGFANASLESIFGYVLAPLAWIMGVDWSDANLAGSLIGQKLAINEFVA
                     YLSFSPYLQTGGTLEVKTIAIISFALCGFANFGSIGVVVGAFSAISPKRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(14911..15849)
                     /gene="psuG"
                     /locus_tag="PN977_000770"
     CDS             complement(14911..15849)
                     /gene="psuG"
                     /locus_tag="PN977_000770"
                     /EC_number="4.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HDI6040834.1"
                     /translation="MSELKISPELLQISPEVQDALKNKKPVVALESTIISHGMPFPQN
                     AQTAIEVEETIRKQGAVPATIAIIGGVMKVGLSKEEIELLGREGHNVTKVSRRDLPFV
                     VAAGKNGATTVASTMIIAALAGIKVFATGGIGGVHRGAEHTFDISADLQELANTNVTV
                     VCAGAKSILDLGLTTEYLETFGVPLIGYQTKALPAFFCRTSPFEVSIRLDSATEIARA
                     MAVKWQSGLNGGLVVANPIPEQFAMPEESINAAIDQAVAEAEEQGVIGKESTPFLLAR
                     VAELTGGDSLKSNIQLVFNNAILASEIAKEYQRLAG"
     gene            complement(15837..16778)
                     /locus_tag="PN977_000771"
     CDS             complement(15837..16778)
                     /locus_tag="PN977_000771"
                     /EC_number="2.7.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine kinase"
                     /protein_id="HDI6040835.1"
                     /translation="MREKDYVVIIGSANIDVAGYSHESLNYADSNPGKIKFTPGGVGR
                     NIAQNLALLGNKAWLLSAVGSDFYGQSLLTQTNQSGVYVDKCLIVPGENTSSYLSLLD
                     NTGEMLVAINDMNISNAITAEYLAQHREFIQRAKVIVADCNISEEALAWILDNAANVP
                     VFVDPVSAWKCVKVRDRLNQIHTLKPNRLEAETLSGIALSGREDVAKVAAWFHQHGLN
                     RLVLSMGGDGVYYSDISGESGWSAPIKTNVINVTGAGDAMMAGLASCWVDGMPFAESV
                     RFAQGCSSMALSCEYTNNPDLSIANVISLVENVECLN"
     gene            complement(17202..18893)
                     /gene="fruA"
                     /locus_tag="PN977_000772"
     CDS             complement(17202..18893)
                     /gene="fruA"
                     /locus_tag="PN977_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIBC"
                     /protein_id="HDI6040836.1"
                     /translation="MKTLLIIDANLGQARAYMAKTLLGAAARKAKLEIIDNPNDAEMA
                     IVLGDSIPNDSALNGKNVWLGDISRAVAHPELFLSEAKGHAKPYTAPVAATAPVAASG
                     PKRVVAVTACPTGVAHTFMAAEAIETEAKKRGWWVKVETRGSVGAGNAITPEEVAAAD
                     LVIVAADIEVDLAKFAGKPMYRTSTGLALKKTAQELDKAVAEATPYEPAGKAQTATTE
                     GKKESAGAYRHLLTGVSYMLPMVVAGGLCIALSFAFGIEAFKEPGTLAAALMQIGGGS
                     AFALMVPVLAGYIAFSIADRPGLTPGLIGGMLAVSTGSGFIGGIIAGFLAGYIAKLIS
                     TQLKLPQSMEALKPILIIPLISSLVVGLAMIYLIGKPVAGILEGLTHWLQTMGTANAV
                     LLGAILGGMMCTDMGGPVNKAAYAFGVGLLSTQTYGPMAAIMAAGMVPPLAMGLATMV
                     ARRKFDKAQQEGGKAALVLGLCFISEGAIPFAARDPMRVLPCCIVGGALTGAISMAIG
                     AKLMAPHGGLFVLLIPGAITPVLGYLVAIIAGTLVAGLAYAFLKRPEVDAVAKAA"
     gene            complement(18910..19848)
                     /gene="fruK"
                     /locus_tag="PN977_000773"
     CDS             complement(18910..19848)
                     /gene="fruK"
                     /locus_tag="PN977_000773"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HDI6040837.1"
                     /translation="MSRRVATITLNPAYDLVGFCPEIERGEVNLVKTTGLHAAGKGIN
                     VAKVLKDLGIDVTVGGFLGKDNQDGFQQLFSELGIANRFQVVQGRTRINVKLTEKDGE
                     VTDFNFSGFEVTPADWERFVTDSLSWLGQFDMVCVSGSLPSGVSPEAFTDWMTRLRSQ
                     CPCIIFDSSREALVAGLKAAPWLVKPNRRELEIWAGRKLPEMKDVIEAAHALREQGIA
                     HVVISLGAEGALWVNASGEWIAKPPSVDVVSTVGAGDSMVGGLIYGLLMRESSEHTLR
                     LATAVAALAVSQSNVGITDRPQLAAMMARVDLQPFN"
     gene            complement(19848..20978)
                     /gene="fruB"
                     /locus_tag="PN977_000774"
     CDS             complement(19848..20978)
                     /gene="fruB"
                     /locus_tag="PN977_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused PTS fructose transporter subunit IIA/HPr
                     protein"
                     /protein_id="HDI6040838.1"
                     /translation="MFQLSVQDIHPGEKAGDKEEAIRQVAAALVQAGNVAEGYVNDML
                     AREQQTSTFLGNGIAIPHGTTDTRDQVLKTGVQVFQFPEGVTWGDGQVAYVAIGIAAS
                     SDEHLGLLRQLTHVLSDDSVAEQLKSATTAEELRALLMGEKQSEQLKLDNEMLTLDIV
                     ASDLLTLQALNAARLKEAGAVDATFVTKAINEQPLNLGQGIWLSDSAEGNLRSAIAVS
                     RAANAFDVDGETAAMLVSVAMNDDQPIAVLKRLADLLLDNKADRLLKADAATLLALLT
                     SDDAPTDDVLSAEFVVRNEHGLHARPGTMLVNTIKQFNSDITVTNLDGTGKPANGRSL
                     MKVVALGVKKGHRLRFTAQGADAEQALKAIGDAIAAGLGEGA"
     gene            21346..22527
                     /gene="setB"
                     /locus_tag="PN977_000775"
     CDS             21346..22527
                     /gene="setB"
                     /locus_tag="PN977_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetB"
                     /protein_id="HDI6040839.1"
                     /translation="MHNSPAVSSAKSFDLTSTAFLIVAFLTGIAGALQTPTLSIFLTD
                     EVHARPAMVGFFFTGSAVIGILVSQFLAGRSDKRGDRKSLIVFCCLLGVLACTLFAWN
                     RNYFVLLFVGVFLSSFGSTANPQMFALAREHADKTGREAVMFSSFLRAQVSLAWVIGP
                     PLAYALAMGFSFTVMYLSAAVAFIVCGVMVWLFLPSMQKELPLATGTVEAPRRNRRDT
                     LLLFVICTLMWGSNSLYIINMPLFIINELHLPEKLAGVMMGTAAGLEIPTMLIAGYFA
                     KRLGKRFLMRVAAVGGVCFYAGMLMAHSPAILLGLQLLNAIFIGILGGIGMLYFQDLM
                     PGQAGSATTLYTNTSRVGWIIAGSVAGIVAEIWNYHAVFWFAMVMIIATLFCLLRIKD
                     V"
     gene            complement(22524..22778)
                     /locus_tag="PN977_000776"
     CDS             complement(22524..22778)
                     /locus_tag="PN977_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HDI6040840.1"
                     /translation="MECRPGCGACCTAPSISSPIPGMPDGKPANTPCIQLDEQQRCKI
                     FTSPLRPKVCAGLQASAEMCGNSRQQAMTWLIDLEMLTAP"
     gene            22933..23505
                     /gene="yeiP"
                     /locus_tag="PN977_000777"
     CDS             22933..23505
                     /gene="yeiP"
                     /locus_tag="PN977_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007750416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P-like protein YeiP"
                     /protein_id="HDI6040841.1"
                     /translation="MPRANEIKKGMVLNYNGKLLLVKDIDIQSPTARGAATLYKMRFS
                     DVRTGLKVEERFKGDDIVDTVTLTRRYVDFSYVDGNEYVFMDKEDYTPYTFTKDQIEE
                     ELLFMPEGGMPDMQVLTWDGQLLALELPQTVDLEIVETAPGIKGASASARNKPATLST
                     GLVIQVPEYLSPGEKIRIHIEERRYMGRAD"
     gene            23728..25194
                     /locus_tag="PN977_000778"
     CDS             23728..25194
                     /locus_tag="PN977_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HDI6040842.1"
                     /translation="MNTIASVTLPHHVHAPRYDRQQLQSRIVHFGFGAFHRAHQALLT
                     DRVLNAQGGDWGICEISLFSGDQLMSQLRAQNHLYTVLEKGADGNQAIIVGAVHDCLN
                     AKLDSLAAIIEKFCEPQVAIVSLTITEKGYCIDPATGALDTSNPRIIHDLQNPEEPHS
                     APGILVEALKRRRERGLAPFTVLSCDNIPDNGHLVKNAVLGMAEKRSPELAGWIKEHV
                     SFPGTMVDRIVPAATDESLAEISQHLGVNDPCAISCEPFIQWVVEDNFVAGRPAWEVA
                     GVQMVNDVLPWEEMKLRMLNGSHSFLAYLGYLSGFAHISDCMQDRAFRHAARTLMLDE
                     QAPTLRIKDFDLTQYADKLIARFANPALKHKTWQIAMDGSQKLPQRMLAGIRIHLARE
                     TDWSLLALGVAGWMRYVSGVDDAGNAIDVRDPLSDKICELVAGSNSEQRVTALLSLRE
                     VFSDDLPDNPHFVQAIEQAWQQIAQFGAHQALLNTLKI"
     gene            25312..26298
                     /gene="yeiR"
                     /locus_tag="PN977_000779"
     CDS             25312..26298
                     /gene="yeiR"
                     /locus_tag="PN977_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding GTPase YeiR"
                     /protein_id="HDI6040843.1"
                     /translation="MTRTNLITGFLGSGKTTSILHLLAHKDPNEKWAVLVNEFGEVGI
                     DGALLADSGALLKEIPGGCMCCVNGLPMQVGLNTLLRQGKPDRLLIEPTGLGHPKQIL
                     DLLTAPVYEPWIDLRATLCILDPRLLLDEKSASNENFRDQLAAADIIVANKSDRATPE
                     SEQALQRWWQQNGGDRQLIHSAHGKVDGHLLDLPRRNLAELPASAAHSHQHVVKKGLA
                     ALSLPEHQRWRRSLNSGQGYQACGWIFDADTVFDTIGILEWARLAPVERVKGVLRIPE
                     GLVRINRQGDDLHIETQNVAPPDSRIELISSSEADWNALQSALLKLRLATTA"
     gene            26337..27050
                     /gene="lpxT"
                     /locus_tag="PN977_000780"
     CDS             26337..27050
                     /gene="lpxT"
                     /locus_tag="PN977_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kdo(2)-lipid A phosphotransferase"
                     /protein_id="HDI6040844.1"
                     /translation="MIKNLPQIVLLNIVGLALFLSWYIPVNHGFWLPIDADIFYFFNQ
                     KLVESKAFLWLVALTNNRAFDGCSLLAMGMLMLSFWLKENAPGRRRIVIIGLVMLLTA
                     VVLNQLGQALIPVKRASPTLTFTDINRVSELLSVPTKDASRDSFPGDHGMMLLIFSAF
                     MWRYFGKVAGLIALIIFVVFAFPRVMIGAHWFTDIIVGSMTVILIGLPWVLLTPLSDR
                     LITFFDKSLPGKNKHFQNK"
     gene            27462..28028
                     /gene="mepS"
                     /locus_tag="PN977_000781"
     CDS             27462..28028
                     /gene="mepS"
                     /locus_tag="PN977_000781"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional murein DD-endopeptidase/murein
                     LD-carboxypeptidase"
                     /protein_id="HDI6040845.1"
                     /translation="MVKSQPILRYILRGIPAIAVAVLLSACSANNTAKNMHPETRAVG
                     SETSSLQASQDEFENLVRNVDVKSRIMDQYADWKGVRYRLGGSTKKGIDCSGFVQRTF
                     REQFGLELPRSTYEQQEMGKSVSRSNLRTGDLVLFRAGSTGRHVGIYIGNNQFVHAST
                     SSGVIISSMNEPYWKKRYNEARRVLSRS"
     gene            28209..29765
                     /locus_tag="PN977_000782"
     CDS             28209..29765
                     /locus_tag="PN977_000782"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
                     /protein_id="HDI6040846.1"
                     /translation="MFIRAPNSGRKLLLTCIVAGVMIAILVSCLQFLVAWHKHEVKYD
                     TLITDVQKYLDTYFADLKSTTDRLQPLTLDTCQQANPELTARAAFSMNVRTFVLVKDK
                     KTFCSSATGEMDIPLKELIPALDINKNVDMAILPGTPMVPNKPAIVIWYRNPLLKNSG
                     VFAALNLNLTPSLFYSSRQEDYDGLALIIGNTALSTFSSRLMNVNELTDMPVRETKIA
                     GIPLTVRLYADDWTWNDVWYAFLLGGMSGTFVGLLCYYLMSVRMRPGREIMTAIKREQ
                     FYVVYQPVVDTQALRVTGLEVLLRWRHPVAGEIPPDAFINFAEAQKMIVPLTQHLFEL
                     IARDAAELEKVLPVGVKFGINIAPAHLHSESFKADIQKLLTSLPAHHFQIVLEITERD
                     MLKEREATQLFAWLHSVGVEIAIDDFGTGHSALIYLERFTLDYLKIDRGFINAIGTET
                     ITSPVLDAVLTLAKRLNMLTVAEGVETPEQARWLSERGVNFMQGYWISRPLPLDDFVR
                     WLKKPYTPQW"
     gene            29847..31661
                     /locus_tag="PN977_000783"
     CDS             29847..31661
                     /locus_tag="PN977_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311096.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDI6040847.1"
                     /translation="MIVRILLLFIALFTFGAQAQAIKESYAFAVLGEPRYAFNFNHFD
                     YVNPAAPKGGQITLSALGTFDNFNRYALRGNPGARTEQLYDTLFTTSDDEPGSYYPLI
                     AESARYADDYSWVEVAINPRARFHDGSPITARDVEFTFQKFMTEGVPQFRLVYKGTTV
                     KAIAPLTVRIELAKPGKEDMLSLFSLPVFPEKYWKDHKLSDPLATPPLASGPYRITSW
                     KMGQNIVYSRVKDYWAANLPVNRGRWNFDTIRYDYYLDDNVAFEAFKAGAFDLRMEND
                     AKNWATRYTGKNFDKKYIIKDEQKNESAQDTRWLAFNIQRPVFSDRRVREAITLAFDF
                     EWMNKALFYNAWSRTNSYFQNTEYAARNYPDAAELVLLAPMKKDLPPEVFTQIYQPPV
                     SKGDGYDRDNLLKADKLLNEAGWVLKGQQRVNATTGQPLSFELLLPSSSNSQWVLPFQ
                     HSLQRLGINMDIRKVDNSQITNRMRSRDYDMMPRVWRAMPWPSSDLQISWSSEYINST
                     YNAPGVQSPVIDSLINQIIAAQGNKEKLLPLGRALDRVLTWNYYMLPMWYMAEDRLAW
                     WDKFSHPAVRPVYSLGIDTWWYDVNKATKLPSARQQGE"
     gene            31662..32756
                     /locus_tag="PN977_000784"
     CDS             31662..32756
                     /locus_tag="PN977_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter permease YejB"
                     /protein_id="HDI6040848.1"
                     /translation="MGAYLIRRLLLVIPTLWAIITINFFIVQIAPGGPVDQAIAAIEF
                     GNAGVLPGAGGEGVRASHAQTGVGNISDSNYRGGRGLDPEVIAEITHRYGFDKPIHER
                     YFKMLWDYIRFDFGDSLFRSASVLTLIKDSLPVSITLGLWSTLIIYLVSIPLGIRKAV
                     YNGSRFDVWSSAFIIIGYAIPAFLFAILLIVFFAGGSYFDLFPLRGLVSANFDSLPWY
                     QKITDYLWHITLPVLATVIGGFAALTMLTKNSFLDEVRKQYVVTARAKGVSEKNILWK
                     HVFRNAMLLVIAGFPATFISMFFTGSLLIEVMFSLNGLGLLGYEATVSRDYPVMFGTL
                     YIFTLIGLLLNIVSDISYTLVDPRIDFEGR"
     gene            32756..33781
                     /locus_tag="PN977_000785"
     CDS             32756..33781
                     /locus_tag="PN977_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDI6040849.1"
                     /translation="MPRLSPVNQARWARFRHNRRGYWSLWIFLVLFGLSLCSELIAND
                     KPLLVRYDGSWYFPLLKNYSESDFGGPLASQADYQDPWLKQRLENNGWVLWAPIRFGA
                     TSINFATDKPFPAPPSRQNWLGTDANGGDVLARILYGTRISVLFGLMLTLCSSVMGVL
                     AGALQGYYGGKVDLWGQRFIEVWSGMPTLFLIILLSSVVQPNFWWLLAITVLFGWMSL
                     VGVVRAEFLRTRNFDYIRAAQALGVSDRSIILRHMLPNAMVATLTFLPFILCSSITTL
                     TSLDFLGFGLPLGSPSLGELLLQGKNNLQAPWLGITAFLSVAILLSLLIFIGEAVRDA
                     FDPNKAV"
     gene            33783..35372
                     /gene="yejF"
                     /locus_tag="PN977_000786"
     CDS             33783..35372
                     /gene="yejF"
                     /locus_tag="PN977_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter ATP-binding protein
                     YejF"
                     /protein_id="HDI6040850.1"
                     /translation="MTQTLLAIENLSVGFRHQQTVRTVVNDVSLQIEAGETLALVGES
                     GSGKSVTALSILRLLPSPPVEYLSGDIRFHGESLLHASDQTLRGVRGNKIAMIFQEPM
                     VSLNPLHTLEKQLYEVLSLHRGMRREAARGEILNCLDRVGIRQAAKRLTDYPHQLSGG
                     ERQRVMIAMALLTRPELLIADEPTTALDVSVQAQILQLLRELQGELNMGMLFITHNLS
                     IVRKLAHRVAVMQNGRCVEQNNAATLFASPTHPYTQKLLNSEPSGDPVPLPEPASTLL
                     DVEQLQVAFPIRKGILKRIVDHNVVVKNISFTLRAGETLGLVGESGSGKSTTGLALLR
                     LINSHGSIVFDGQPLQNLNRRQLLPIRHRIQVVFQDPNSSLNPRLNVLQIIEEGLRVH
                     QPTLSAAQREQQVIAVMHEVGLDPETRHRYPAEFSGGQRQRIAIARALILKPSLIILD
                     EPTSSLDKTVQAQILTLLKSLQQKHQLAYLFISHDLHVVRALCHQVIVLRQGEVVEQG
                     PCARVFAAPQQEYTRQLLALS"
     gene            complement(35376..35720)
                     /locus_tag="PN977_000787"
     CDS             complement(35376..35720)
                     /locus_tag="PN977_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6040851.1"
                     /translation="MTSLQLSIVHRLPQNYRWSAGFAGSKVEPIPQNGPCGDNSLVAL
                     KLLSPDGDNAWSVMYKLSQALSDIEVPCSVLECEGEPCLFVNRQDEFAATCRLKNFGV
                     AIAEPFSNYNPF"
     gene            complement(36054..37244)
                     /gene="bcr"
                     /locus_tag="PN977_000788"
     CDS             complement(36054..37244)
                     /gene="bcr"
                     /locus_tag="PN977_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter Bcr"
                     /protein_id="HDI6040852.1"
                     /translation="MTTRQHSSFAIVFILGLLAMLMPLSIDMYLPALPVISAQFGVPA
                     GSTQMTLSTYILGFALGQLIYGPMADSFGRKPVVLGGTLVFAAAAVACALAQTIDQLI
                     VMRFFHGLAAAAASVVINALMRDIYPKEEFSRMMSFVMLVTTIAPLMAPIVGGWVLVW
                     LSWHYIFWILAVAAILASAMIFFLIKETLPPERRQPFHIRTTIGNFAALFRHKRVLSY
                     MLASGFSFAGMFSFLSAGPFVYIEINHVAPENFGYYFALNIVFLFVMTIFNSRFVRRI
                     GALNMFRSGLWIQFIMAAWMVISALLGLGFWSLVVGVAAFVGCVSMVSSNAMAVILDE
                     FPHMAGTASSLAGTFRFGIGAIVGALLSLATFNSAWPMIWSIAFCATSSILFCLYASR
                     PKKR"
     gene            complement(37272..37967)
                     /gene="rsuA"
                     /locus_tag="PN977_000789"
     CDS             complement(37272..37967)
                     /gene="rsuA"
                     /locus_tag="PN977_000789"
                     /EC_number="5.4.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase RsuA"
                     /protein_id="HDI6040853.1"
                     /translation="MRLDKFIAQQLGVSRAIAGREIRGNRVTVDGEIVRNAAFKLLPE
                     HDVAYDGNPLAQQHGPRYFMLNKPQGYVCSTDDPDHPTVLYFLDEPVAWKLHAAGRLD
                     IDTTGLVLMTDDGQWSHRITSPRHHCEKTYLVTLESPVADDTAEQFAKGVQLHNEKDL
                     TKPAVLEVITPTQVRLTISEGRYHQVKRMFAAVGNHVVELHRERIGGITLDADLAPGE
                     YRPLTEEEIASVV"
     gene            38117..39877
                     /locus_tag="PN977_000790"
     CDS             38117..39877
                     /locus_tag="PN977_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HDI6040854.1"
                     /translation="MIFTLRPYQQEAVDATLNHFRRHKTPAVIVLPTGAGKSLVIAEL
                     ARLARGRVLVLAHVKELVAQNHAKYQALGLEADIFAAGLKRKESHGKVVFGSVQSVAR
                     NLDAFQGEFSLLIVDECHRIGDDEESQYQQILTHLTKVNPHLRLLGLTATPFRLGKGW
                     IYQFHYHGMVRGDEKALFRDCIYELPLRYMIKHGYLTPPERLDMPVVQYDFSRLQAQS
                     NGLFSEADLNRELKKQQRITPHIINQIMEFAATRKGVMIFAATVEHAKEIVGLLPTED
                     AALITGDTPGAERDVLIEDFKAQRFRYLVNVAVLTTGFDAPHVDLIAILRPTESVSLY
                     QQIVGRGLRLAPGKTDCLILDYAGNPHDLYAPEVGTPKGKSDNVPVQVFCPACGFANT
                     FWGKTTADGTLIEHFGRRCQGWFEDDDGHREQCDFRFRFKNCPQCNAENDIAARRCRE
                     CDTILVDPDDMLKAALRLKDALVLRCSGMSLQHGHDEKGEWLKITYYDEDGADVSERF
                     RLQTPAQRTAFEQLFIRPHTRTPGIPLRWITAADILAQQALLRHPDFVVARMKGQYWQ
                     VREKVFDYEGRFRRAHELRG"
     gene            40002..40286
                     /gene="rplY"
                     /locus_tag="PN977_000791"
     CDS             40002..40286
                     /gene="rplY"
                     /locus_tag="PN977_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000494192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HDI6040855.1"
                     /translation="MFTINAEVRKEQGKGASRRLRAANKFPAIIYGGKEAPLAVELDH
                     DKVMNMQVKAEFYSEVLTIVVDGKEIKVKAQDVQRHPYKPKLLHIDFVRA"
     gene            complement(40425..41432)
                     /gene="yejK"
                     /locus_tag="PN977_000792"
     CDS             complement(40425..41432)
                     /gene="yejK"
                     /locus_tag="PN977_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein YejK"
                     /protein_id="HDI6040856.1"
                     /translation="MSLDINQIALHQLIKRDEQNLELVLRDSLLEPTETVVEMVAELH
                     RVYSAKNKAYGLFSEESELAQTLRLQRQGEEDFLAFSRAATGRLRDELAKYPFADGGF
                     VLFCHYRYLAVEYLLVAVLSNLSSMRVNENLDINPTHYLDINHADIVARIDLTEWETN
                     PESTRYLTFLKGRVGRKVADFFMDFLGASEGLNAKAQNRGLLQAVDDFTAEAQLDKAE
                     RQNVRQQVYSYCNEQLQAGEEIELESLSKELAGVSEVSFTEFAAEKGYELEESFPADR
                     STLRQLTKFAGSGGGLTINFDAMLLGERIFWDPATDTLTIKGTPPNLRDQLQRRTSGG
                     N"
     gene            41614..41841
                     /locus_tag="PN977_000793"
     CDS             41614..41841
                     /locus_tag="PN977_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YejL family protein"
                     /protein_id="HDI6040857.1"
                     /translation="MPQISRYSDEQVEQLLAELLNILEKHKAPTDLSLMVLGNMVTNL
                     INTSIAPAQRQAIANSFARALQSSINEDKAH"
     gene            41861..43621
                     /gene="pbgA"
                     /locus_tag="PN977_000794"
     CDS             41861..43621
                     /gene="pbgA"
                     /locus_tag="PN977_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin transport protein PbgA"
                     /protein_id="HDI6040858.1"
                     /translation="MVTHRQRYREKVSQMVSWGHWFALFNILLSLVIGSRYLFIADWP
                     TTLAGRIYSYVSIIGHFSFLVFATYLLILFPLTFIVGSQRLMRFLSVILATAGMTLLL
                     IDSEVFTRFHLHLNPIVWQLVINPDENEMARDWQLMFISVPVILLLELVFATWSWQKL
                     RSLTRRRRFARPLAAFLFIAFIASHVVYIWADANFYRPITMQRANLPLSYPMTARRFL
                     EKHGLLDAQEYQRRLIEQGNPDAVSVQYPLSELRYRDMGTGQNVLLITVDGLNYSRFE
                     KQMPALAGFAEQNISFTRHMSSGNTTDNGIFGLFYGISPSYMDGILSTRTPAALITAL
                     NQQGYQLGLFSSDGFTSPLYRQALLSDFSMPSIRTQSDEQTATQWINWLGRYAQEDNR
                     WFSWVSFNGTNIDDSNQQAFARKYSRAAGNVDDQINRVLNALRDSGKLDNTVVIITAG
                     RGIPLSEEEETFDWSHGHLQVPLVIHWPGTPAQRINALTDHTDLMTTLMQRLLHVSTP
                     ASEYSQGQDLFNPQRRHYWVTAADNDTLAITTPKKTLVLNNNGKYRTYNLRGERVKDE
                     KPQLSLLLQVLTDEKRFIAN"
     gene            43696..43772
                     /locus_tag="PN977_000795"
     tRNA            43696..43772
                     /locus_tag="PN977_000795"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:43730..43732,aa:Pro,seq:ggg)
     gene            complement(43875..46466)
                     /gene="yejO"
                     /locus_tag="PN977_000796"
     CDS             complement(43875..46466)
                     /gene="yejO"
                     /locus_tag="PN977_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311108.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter YejO"
                     /protein_id="HDI6040859.1"
                     /translation="MHQSGSVSLCRSAISVLVATALYSPIALASTVEYGETVDGVVLE
                     KDIQLVYGTANNTKINPGGEQHIKEFGISSNTEINGGYQYIEMNGTAEYSVLNDGYQI
                     VQMGGAANQTTLNNGVLQVYGAANEPTIKGGRLIVEKDGGTVFAAIEKGGLLEVKEGG
                     FALAVDQKAGGAIKTTTRAMEVFGTNRLGQFDIKNGIANNMLLENGGSLRVEENDFAY
                     NTTVDNGGLLEVMDGGTATGVDKKAGGKLIVSTNALEVSGTNSKGQFSIKDGVSKNYE
                     LDDGSGLIVMEDTQAIDTILDEHATMQSLGKDTGTRVQANAVYDLGRSDQNGSITYSS
                     KAISENMVINNGRANVWAGTMVNVSVRGNDGILEVMKPQINYAPAMLVGSVVVSEGAS
                     LRTHGAVDTSKADVSLDGSSWTIIADITTTNQNTLLNLANLAMSDANVIMMDEPVTRS
                     SVTASAENFITLTTNTLSGNGNFYMRTDMANHQSDQLNVTGQATGDFKIFVTDTGASP
                     AAGDSLTLVTTGGGDAAFTLGNAGGVVDIGTYEYTLLDNGNHSWSLAENRAQITPSTT
                     DVLNMAAAQPLVFDAELDTVRERLGSVKGVNYDTAMWSSAINTRNNVTTDAGAGFEQT
                     LTGLTLGIDSRFSREESSTIRGLFFGYSHSDIGFDRGGKGNIDSYTLGAYAGWEHQNG
                     AYVDGVVKVDRFANTIHGKMSNGATAFGDYNSNGAGAHVESGFRWVDGLWSVRPYLAF
                     TGFTTDGQDYTLSNGMRADVGNTRILRAEAGTAVSYHMDLQNGTTLEPWLKAAVRQEY
                     ADSNQVKVNDDGKFNNDVAGTRGVYQAGIRSSFTPTLSGHLSVSYGNGAGVESPWNTQ
                     AGVVWTF"
     gene            46786..47433
                     /gene="narP"
                     /locus_tag="PN977_000797"
     CDS             46786..47433
                     /gene="narP"
                     /locus_tag="PN977_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite response regulator protein NarP"
                     /protein_id="HDI6040860.1"
                     /translation="MPEATPFQVMIVDDHPLMRRGVRQLLELDPGFEVVAEAGDGASA
                     IDLANRLDIDVILLDLNMKGMSGLDTLNALRRDGVTAQIIILTVSDASSDVFALIDAG
                     ADGYLLKDSDPEVLLEAIRAGAKGSKVFSERVNQYLREREMFGAEEDPFSVLTERELD
                     VLHELAQGLSNKQIASVLNISEQTVKVHIRNLLRKLNVRSRVAATILFLQQRGAQ"
     gene            complement(47468..48520)
                     /gene="ccmH"
                     /locus_tag="PN977_000798"
     CDS             complement(47468..48520)
                     /gene="ccmH"
                     /locus_tag="PN977_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis thiol:disulfide
                     oxidoreductase CcmH"
                     /protein_id="HDI6040861.1"
                     /translation="MRFLLGVLMLMISGSALATIDVLQFKDEAQEQQFRQLTEELRCP
                     KCQNNSIADSNSMIATDLRQKVYELMQEGKSKKEIVDYMVARYGNFVTYDPPLTPLTV
                     LLWVLPVVAIGIGGWVIYARSRRRVRVVPDAFPEQSVPEGKRAGYIVYLPGIVVALIV
                     AGVSYYQTGNYQQVKIWQQATAQAPALLDRALDPKADPLNEEEMSRLALGMRTQLQKN
                     PGDIEGWIMLGRVGMALGNASIATDAYATAYRLDPKNSDAALGYAEALTRSSDPNDNR
                     LGGELLRQLVRTDHSNIRVLSMYAFNAFEQQRFGEAVAAWEMMLKLLPANDTRRAVIE
                     RSIAQAMQHLSPQESK"
     gene            complement(48517..49074)
                     /gene="dsbE"
                     /locus_tag="PN977_000799"
     CDS             complement(48517..49074)
                     /gene="dsbE"
                     /locus_tag="PN977_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbE"
                     /protein_id="HDI6040862.1"
                     /translation="MKRKVLLIPLIIFLAIAAALLWQLARNAEGDDPTNLESALIGKP
                     VPKFRLESLDNPGQFYQADVLTQGKPVLLNVWATWCPTCRAEHQYLNQLSAQGIRVVG
                     MNYKDDRQKAISWLKELGNPYALSLFDGDGMLGLDLGVYGAPETFLIDGNGIIRYRHA
                     GDLNPRVWEEEIKPLWEKYSKEAAQ"
     gene            complement(49071..51014)
                     /gene="ccmF"
                     /locus_tag="PN977_000800"
     CDS             complement(49071..51014)
                     /gene="ccmF"
                     /locus_tag="PN977_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis heme lyase CcmF"
                     /protein_id="HDI6040863.1"
                     /translation="MMPEIGNGLLCLALGIALLLSVYPLWGVARGDARMMASSRLFAW
                     LLFMSVAGAFLVLVNAFVVNDFTVTYVASNSNTQLPVWYRVAATWGAHEGSLLLWVLL
                     MSGWTFAVAIFSQRIPLDIVARVLAIMGMVSVGFLLFILFTSNPFSRTLPNFPIEGRD
                     LNPLLQDPGLIFHPPLLYMGYVGFSVAFAFAIASLLSGRLDSTYARFTRPWTLAAWIF
                     LTLGIVLGSAWAYYELGWGGWWFWDPVENASFMPWLVGTALMHSLAVTEQRASFKAWT
                     LLLAISAFSLCLLGTFLVRSGVLVSVHAFASDPARGMFILAFMVLVIGGSLLLFAARG
                     HKVRSRVNNALWSRESLLLANNVLLVAAMLVVLLGTLLPLVHKQLGLGSISIGEPFFN
                     TMFTWLMVPFALLLGVGPLVRWGRDRPRKIRNLLIIAFISTLVLSLLLPWLFESKVVA
                     MTVLGLAMACWIAVLAIAEAALRISRGTKTTFSYWGMVAAHLGLAVTIVGIAFSQNYS
                     VERDVRMKSGDSVDIHEYRFTFRDVKEVTGPNWRGGVATIGVTRDGKPETVLYAEKRY
                     YNTAGSMMTEAAIDGGITRDLYAALGEELENGAWAVRLYYKPFVRWIWAGGLMMALGG
                     LLCLFDPRYRKRVSPQKTAPEAV"
     gene            complement(51011..51490)
                     /gene="ccmE"
                     /locus_tag="PN977_000801"
     CDS             complement(51011..51490)
                     /gene="ccmE"
                     /locus_tag="PN977_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HDI6040864.1"
                     /translation="MNIRRKNRLWIACAVLAGLALTIGLVLYALRSNIDLFYTPGEIL
                     YGKRETQQMPEVGQRLRVGGMVMPGSVQRDPNSLKVTFTIYDAEGSVDVSYEGILPDL
                     FREGQGVVVQGELEEGNHILAKEVLAKHDENYTPPEVEKAMEANHRRPASVYKDPAS"
     gene            complement(51487..51696)
                     /gene="ccmD"
                     /locus_tag="PN977_000802"
     CDS             complement(51487..51696)
                     /gene="ccmD"
                     /locus_tag="PN977_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmD"
                     /protein_id="HDI6040865.1"
                     /translation="MTPAFASWNEFFAMGGYAFFVWLAVVMTVIPLVVLVVHSVMQHR
                     AILRGVAQQRAREARLRAAQQQEAA"
     gene            complement(51693..52430)
                     /gene="ccmC"
                     /locus_tag="PN977_000803"
     CDS             complement(51693..52430)
                     /gene="ccmC"
                     /locus_tag="PN977_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmC"
                     /protein_id="HDI6040866.1"
                     /translation="MWKTLHQLAIPPRLYQICGWFIPWLAIASVVVLTVGWIWGFGFA
                     PADYQQGNSYRIIYLHVPAAIWSMGIYASMAVAAFIGLVWQMKMANLAVAAMAPIGAV
                     FTFIALVTGSAWGKPMWGTWWVWDARLTSELVLLFLYVGVIALWHAFDDRRLAGRAAG
                     ILVLIGVVNLPIIHYSVEWWNTLHQGSTRMQQSIDPAMRSPLRWSIFGFLLLSATLTL
                     MRMRNLILLMEKRRPWVSELILKRGRK"
     gene            complement(52472..53134)
                     /gene="ccmB"
                     /locus_tag="PN977_000804"
     CDS             complement(52472..53134)
                     /gene="ccmB"
                     /locus_tag="PN977_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HDI6040867.1"
                     /translation="MMFWRIFRLELRVAFRHSAEIANPLWFFLIVITLFPLSIGPEPQ
                     LLARIAPGIIWVAALLSSLLALERLFRDDLQDGSLEQLMLLPLPLPAVVLAKVMAHWM
                     VTGLPLLILSPLVAMLLGMDVYGWQVMALTLLLGTPTLGFLGAPGVALTVGLKRGGVL
                     LSILVLPLTIPLLIFATAAMDAASMHLPVDGYLAILGALLAGTATLSPFATAAALRIS
                     IQ"
     gene            complement(53131..53748)
                     /gene="ccmA"
                     /locus_tag="PN977_000805"
     CDS             complement(53131..53748)
                     /gene="ccmA"
                     /locus_tag="PN977_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001300065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis heme-transporting ATPase
                     CcmA"
                     /protein_id="HDI6040868.1"
                     /translation="MLEARELLCERDERTLFSGLSFTLNAGEWVQITGSNGAGKTTLL
                     RLLTGLSRPDAGDVLWQGQPLHQVRDSYHQNLLWIGHQPGIKTRLTALENLHFYHRDG
                     DTAQCLEALAQAGLAGFEDIPVNQLSAGQQRRVALARLWLTRATLWILDEPFTAIDVN
                     GVDRLTQRMAQHTEQGGTVILTTHQPLNVAESKIRRISLTQTGAA"
     gene            complement(53767..54369)
                     /gene="napC"
                     /locus_tag="PN977_000806"
     CDS             complement(53767..54369)
                     /gene="napC"
                     /locus_tag="PN977_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type protein NapC"
                     /protein_id="HDI6040869.1"
                     /translation="MGNSDRKPGLIKRLWKWWRTPSRLALGTLLLIGFVGGIVFWGGF
                     NTGMEKANTEEFCISCHEMRNTVYQEYMDSVHYNNRSGVRATCPDCHVPHEFVPKMIR
                     KLKASKELYGKIFGVIDTPQKFEAHRLTMAQNEWRRMKDNNSQECRNCHNFEYMDTTA
                     QKSVAAKMHDQAVKDGQTCIDCHKGIAHKLPDMREVEPGF"
     gene            complement(54379..54828)
                     /gene="napB"
                     /locus_tag="PN977_000807"
     CDS             complement(54379..54828)
                     /gene="napB"
                     /locus_tag="PN977_000807"
                     /EC_number="1.9.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase cytochrome c-type subunit"
                     /protein_id="HDI6040870.1"
                     /translation="MKSHDLKKALCQWTAMLALVVSGAVWAANGVDFSQSPEVSGTQE
                     GAIRMPKEQERMPLNYVNQPPMIPHSVEGYQVTTNTNRCLQCHGVESYRTTGAPRISP
                     THFMDSDGKVGAEVAPRRYFCLQCHVPQADTAPIVGNTFTPSKGYGK"
     gene            complement(54825..55688)
                     /gene="napH"
                     /locus_tag="PN977_000808"
     CDS             complement(54825..55688)
                     /gene="napH"
                     /locus_tag="PN977_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinol dehydrogenase ferredoxin subunit NapH"
                     /protein_id="HDI6040871.1"
                     /translation="MANRKRDAGREALEKKGWWRSHRWLVLRRLCQFFVLGMFLSGPW
                     FGVWILHGNYSSSLLFDTVPLTDPLMTLQSLASGHLPATVALTGAVIITVLYALAGKR
                     LFCSWVCPLNPITDLANWLRRRFDLNQSATIPRHIRYVLLVVILLGSALTGTLIWEWI
                     NPVSLMGRSLVMGFGSGALLILALFLFDLLVVEHGWCGHICPVGALYGVLGSKGVITV
                     AASDRQKCNRCMDCFHVCPEPHVLRAPVLDEQSPVQVTSRDCMTCGRCVDVCSEDVFT
                     ITTRWSSGAKS"
     gene            complement(55675..56370)
                     /gene="napG"
                     /locus_tag="PN977_000809"
     CDS             complement(55675..56370)
                     /gene="napG"
                     /locus_tag="PN977_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapG"
                     /protein_id="HDI6040872.1"
                     /translation="MSRSAKPQNGRRRFLRDVVRTAGGLAAVGVALGLQQQTARASGV
                     RLRPPGAINENAFASACVRCGQCVQACPYDTLKLATLASGLSAGTPYFVARDIPCEMC
                     EDIPCAKVCPSGALDREIESIDDARMGLAVLVDQENCLNFQGLRCDVCYRECPKIDEA
                     ITLELERNTRTGKHARFLPTVHSDACTGCGKCEKVCVLEQPAIKVLPLSLAKGELGHH
                     YRFGWLEGNNGKS"
     gene            complement(56377..58863)
                     /gene="napA"
                     /locus_tag="PN977_000810"
     CDS             complement(56377..58863)
                     /gene="napA"
                     /locus_tag="PN977_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="periplasmic nitrate reductase subunit alpha"
                     /protein_id="HDI6040873.1"
                     /translation="MKLSRRSFMKANAVAAAAAAAGLSVPGVARAVVGQQEAIKWDKA
                     PCRFCGTGCGVLVGTQQGRVVACQGDPDAPVNRGLNCIKGYFLPKIMYGKDRLTQPLL
                     RMKNGKYDKEGEFTPITWDQAFDVMEEKFKTALKEKGPESIGMFGSGQWTIWEGYAAS
                     KLFKAGFRSNNIDPNARHCMASAVVGFMRTFGMDEPMGCYDDIEQADAFVLWGANMAE
                     MHPILWSRITNRRLSNQNVTVAVLSTYQHRSFELADNGIIFTPQSDLVILNYIANYII
                     QNNAINQDFFSKHVNLRKGATDIGYGLRPTHPLEKAAKNPGSDASEPMSFEDYKAFVA
                     EYTLEKTAEMTGVPKDQLEQLAQLYADPNKKVISYWTMGFNQHTRGVWANNLVYNLHL
                     LTGKISQPGCGPFSLTGQPSACGTAREVGTFAHRLPADMVVTNEKHRDICEKKWNIPS
                     GTIPAKIGLHAVAQDRALKDGKLNVYWTMCTNNMQAGPNINEERMPGWRDPRNFIIVS
                     DPYPTVSALAADLILPTAMWVEKEGAYGNAERRTQFWRQQVQAPGEAKSDLWQLVQFS
                     RRFKTEEVWPEELLAKKPELRGKTLYEVLYATPEVSKFPLSELAEDQLNDESRELGFY
                     LQKGLFEEYAWFGRGHGHDLAPFDDYHKARGLRWPVVNGKETQWRYSEGNDPYVKAGE
                     GYKFYGKPDGKAVIFALPFEPAAEAPDEEYDLWLSTGRVLEHWHTGSMTRRVPELHRA
                     FPEAVLFIHPLDAKARDLRRGDKVKVVSRRGEVISIVETRGRNRPPQGLVYMPFFDAA
                     QLVNKLTLDATDPLSKETDFKKCAVKLEKV"
     gene            complement(58860..59123)
                     /gene="napD"
                     /locus_tag="PN977_000811"
     CDS             complement(58860..59123)
                     /gene="napD"
                     /locus_tag="PN977_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone NapD"
                     /protein_id="HDI6040874.1"
                     /translation="MHTNWQVCSLVVQAKSERISDISTQLNAFTGCEVAVSDAPSGQL
                     IVVVEAEDSETLIQTIESVRNVEGVLAVSLVYHQQEEQGEETP"
     gene            complement(59113..59607)
                     /gene="napF"
                     /locus_tag="PN977_000812"
     CDS             complement(59113..59607)
                     /gene="napF"
                     /locus_tag="PN977_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapF"
                     /protein_id="HDI6040875.1"
                     /translation="MKIDASRRGILTGRWRKASNGIRPPWSGDESHFLTHCTRCDVCI
                     NACENNILQRGAGGYPSVNFKNNECSFCYACAQACPESLFSPRHTRAWDLQFTIGDAC
                     LAYQSVECRRCQDSCEPMAIIFRPTLSGIYQPQLNSQLCNGCGACAASCPVSAITAEY
                     LHAH"
     gene            59716..59880
                     /locus_tag="PN977_000813"
     CDS             59716..59880
                     /locus_tag="PN977_000813"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6040876.1"
                     /translation="MHTPIGVKPVAGSKEWREAWQKRAFAHISNGYKHIYIAINSPEI
                     FLLVCFLIRI"
     gene            60015..60503
                     /gene="eco"
                     /locus_tag="PN977_000814"
     CDS             60015..60503
                     /gene="eco"
                     /locus_tag="PN977_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease inhibitor ecotin"
                     /protein_id="HDI6040877.1"
                     /translation="MKTILPAVLFAAFATTSAWAAESVQPLEKIAPYPQAEKGMKRQV
                     IQLTPQEDESTLKVELLIGQTLEVDCNLHRLGGKLESKTLEGWGYDYYVFDKVSSPVS
                     TMMACPDGKKEKKFVTAYLGDAGMLRYNSKLPIVVYTPDNVDVKYRVWKAEEKIDNAV
                     VR"
     gene            complement(60653..62299)
                     /gene="mqo"
                     /locus_tag="PN977_000815"
     CDS             complement(60653..62299)
                     /gene="mqo"
                     /locus_tag="PN977_000815"
                     /EC_number="1.1.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase (quinone)"
                     /protein_id="HDI6040878.1"
                     /translation="MKKVTAMLFSMAVGLNAVSMAAKAKASEEQETDVLLIGGGIMSA
                     TLGTYLRELEPEWSMTMVERLEGVAQESSNGWNNAGTGHSALMELNYTPQNADGSISI
                     EKAVAINEAFQISRQFWAHQVERGVLRTPRSFINTVPHMSFVWGEDNVNFLRARYAAL
                     QQSSLFRGMRYSEDHAQIKEWAPLVMEGRDPQQKVAATRTEIGTDVNYGEITRQLIAS
                     LQKKSNFSLQLSSEVRALKRNDDNTWTVTVADLKNGTAQNIRAKFVFIGAGGAALKLL
                     QESGIPEAKDYAGFPVGGQFLVSENPDVVNHHLAKVYGKASVGAPPMSVPHIDTRVLD
                     GKRVVLFGPFATFSTKFLKNGSLWDLMSSTTTSNVMPMMHVGLDNFDLVKYLVSQVML
                     SEEDRFEALKEYYPQAKKEDWRLWQAGQRVQIIKRDAEKGGVLRLGTEVVSDQQGTIA
                     ALLGASPGASTAAPIMLNLLEKVFGDRVSSPQWQATLKATVPSYGRKLNGDVAATERE
                     LQYTSEVLGLKYDKPQAADSTPKPQLKPQPVQKEVADIAL"
     gene            complement(62517..64160)
                     /gene="yojI"
                     /locus_tag="PN977_000816"
     CDS             complement(62517..64160)
                     /gene="yojI"
                     /locus_tag="PN977_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin J25 efflux ABC transporter YojI"
                     /protein_id="HDI6040879.1"
                     /translation="MELLVLVWRQYRWPFISVMALSLASAALGIGLIAFINQRLIETA
                     DTSLLVLPEFLGLLLLLMAVTLGSQLALTTLGHHFVYRLRSEFIKRILDTHVERIEQL
                     GSASLLAGLTSDVRNITIAFVRLPELVQGIILTIGSAAYLWMLSGKMLLVTAIWMAVT
                     IWGGFVLVARVYKHMATLRETEDKLYTDFQTVLEGRKELTLNRERAEYVFNNLYIPDA
                     QEYRHHIIRADTFHLSAVNWSNIMMLGAIGLVFWMANSLGWADTNVAATYSLTLLFLR
                     TPLLSAVGALPTLLTAQVAFNKLNKFALAPFKAEFPRPQTFPNWQTLELRNVTFAYQD
                     NAFSVGPINLTIKRGELLFLIGGNGSGKSTLAMLLTGLYQPQSGEILLDGKPVSGEQP
                     EDYRKLFSAVFTDVWLFDQLLGPEGQPANPQLIEKWLAQLKMAHKLELSNGRIVNLKL
                     SKGQKKRVALLLALAEERDIILLDEWAADQDPHFRREFYQVLLPLMQEMGKTIFAISH
                     DDHYFIHADRLLEMRNGQLSELTGEERDAASRDAVARTA"
     gene            complement(64236..64886)
                     /gene="alkB"
                     /locus_tag="PN977_000817"
     CDS             complement(64236..64886)
                     /gene="alkB"
                     /locus_tag="PN977_000817"
                     /EC_number="1.14.11.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA oxidative demethylase AlkB"
                     /protein_id="HDI6040880.1"
                     /translation="MLDLFADAEPWQEPLAAGAVILRRFAFNAAEQLIRDINDVASQS
                     PFRQMVTPGGYTMSVAMTNCGHLGWTTHRQGYLYSPIDPQTNKPWPAMPQSFHHLCQR
                     AATAGGYPDFQPDACLINRYVPGAKLSLHQDKDEPDLRAPIVSVSLGLPAIFQFGGLK
                     RNDPLKRLLLEHGDVVVWGGESRLFYHGIQPLKAGFHPLAGDCRYNLTFRQAGKKE"
     gene            complement(64886..>65535)
                     /gene="ada"
                     /locus_tag="PN977_000818"
     CDS             complement(64886..>65535)
                     /gene="ada"
                     /locus_tag="PN977_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional DNA-binding transcriptional
                     regulator/O6-methylguanine-DNA methyltransferase Ada"
                     /protein_id="HDI6040881.1"
                     /translation="LRESLAKGESVTTSILNAGFPDSSSYYRKADETLGMTAKQFRHG
                     GENLAVRYALADCELGRCLVAESERGICAILLGDDDATLISELQQMFPAADSAPADLT
                     FQQHVREVIASLNQRDTPLTLPLDIRGTAFQQQVWQALRTIPCGETVSYQQLANAIGK
                     PKAVRAVASACAANKLAIVIPCHRVVRGDGTLSGYRWGVSRKAQLLRREAENEER"
BASE COUNT        16011 a        17778 c        16143 g        15603 t
ORIGIN      
        1 aaaatagccg atgcatccgg cgtccaggcg cggctggtct ggctgtcgga aatcagcccc
       61 aataacagca aaacgccaag ccccaccaga taaagcatga cgcctgtatt aaacaggctt
      121 tttaccgatg gcgcatcgcg caccagccgc cagcagatta agcccaccag cccgtaagcc
      181 agcagaatat cgccgtccca gaacaataaa ccgtgaataa agcccagcaa gactaacagc
      241 gttaaccgcg actggatcca gcgtctgcca cggggcagca acatttgcag gcccgcacca
      301 aacagcagag caaaaagcgt gaggaatttc acctggccga tgagatcgag aaatgcccag
      361 gtccatgcat cctgcgacgt aatagcgccg taccaggcgg gattgagata agccgccttt
      421 ggtagcccaa aggcgctgat gtttaatagc aggatcccca gaatggcgac gccgcgaaca
      481 aaatcgagcg tgacgttgcg ctccatggtt cctgcctttt aatcagttgt gatgacgcac
      541 agcgcgcaga aactcgtggc gcgtattctg actggatttg aacaatccac caagagaggt
      601 cgttgttgtg gcactggttg cgtcgcggat gccacgcgct ttcacgcagt aatgcaccgc
      661 gtcgatagag acagccacgt tattggtgcc cagcagcgtt tgtagcgcaa tgagaatttg
      721 ctgggtcaga cgttcctgca cctgcggacg ctgggcaaag aactgcacga tgcggttaat
      781 ttttgacaga ccgatcaccg aatctttcgg gatataagcc accgtcgctt tgccatcaat
      841 ggtaacaaaa tggtgttcac aggtactggt cagagtgata tcgcgcaccg taaccatctc
      901 atcgaccttc attttgtttt caataagggt aattttcggg aaattggcgt aatccagacc
      961 ggagaaaatt tcatcgacat acattttggc aatacgatgc ggcgtttcca tcaaactgtc
     1021 atcagccagg tcgagattca gcaactgcat gatttccgtc atatgaccgg cgataaggct
     1081 tttgcgcgtt tcgttatcca tttcatgcac gggcgggcgc agcggtgttt ccagtcctcg
     1141 cgcaaccagc gcttcatgaa ccagggccgc ttctttactg agtgatggca tttatttttc
     1201 tcctgcaggt gtgacgcctc tgccctgcgt ggggcaaagt tattaagctg attagcagcc
     1261 tgattattgt gcgtgaggcg gcacacataa tccagtattc accgcgataa ttgttgtaat
     1321 tgccgctgcc ttacaaaggt gtaatgttaa aacacggtta tgtaaatacg gaagtgaaag
     1381 ttactcacga cacaatgaat taacggtatg atggaggaat tgcaaacaac acaacaagga
     1441 gccacgcatg gaattgctcg aagagcacct ctgttttgaa ggctggcaac aacgctggcg
     1501 acacgactcc agtaccttaa actgcccgat gacgttcagt atctttctcc caccacctcg
     1561 tgatcacacc ccgccaccag tgctgtactg gctttccgga ttaacctgca atgacgagaa
     1621 cttcaccacc aaggcgggtg cccagcgcgt ggcggcggaa ctggggattg tactggtgat
     1681 gccagacacc agcccgcgcg gcgaacaggt tgccaacgac gatggctacg atttaggcca
     1741 gggcgcaggc ttttatctta atgccacgca accgccttgg gcgacgcatt accggatgta
     1801 tgattatctg cgcgatgaat taccggcgct ggttcagtcg caatttaatg tcagcgaccg
     1861 ctgcgccatt agcggtcatt ccatgggtgg tcacggtgcg ctgattatgg cgctgaaaaa
     1921 cccgggcaaa tacaccagcg tttccgcctt tgcgccgatt gtgaatccgt gcagcatccc
     1981 gtggggaatc aaagcgttta gccgctattt aggtgaggac aaaaatgcat ggctggaatg
     2041 ggacagttgc gcgctgatgt atgccagtaa cgcgcaggat gcgatcccga cgcttatcga
     2101 tcagggtgat aatgatcagt ttcttgccga tcagttgcaa cccgcagtgc tggcagaagc
     2161 cgcgcgccag aaagcgtggc cgatgacgct gcgtattcag ccggggtatg atcacagtta
     2221 ctacttcatc gcctctttta tagaggatca cctgcgcttc catgcgcagt atttactgaa
     2281 gtgaaagtcc gcccggttcg ccgggcatct tctcatcaga agcgataatc cactgccata
     2341 aagtaacgac gtccgtcttc gttatagctg tagtcgtcac gactgagatc cttgtcgcca
     2401 aggttcagca cgcctgcgca cagtttgacg tctttagtca cctgccaggc cgcaccggta
     2461 ttccagatgg tataaccgcc cggtgtttta gccgtcgcgc tgtcggcgcg tttctgcccg
     2521 gtatagtgac cagaaacata gaatgaccag tcttccagcg ccagcggttt ccagtccagc
     2581 gtaccgttag cagtatggaa cggcagatcg gatagcggtt tgttttcgcc gttgctgaca
     2641 tcacgaccat cgttgtaggt gtagttgatc gacagtttcc attcatcgtt gaacggaatt
     2701 ttcagttcgg tttccacgcc ctgaatacga gctttgttaa cgttgtagta gctaaatacc
     2761 ggtatgcgcc gtccgttagc gcccgtctca aaaccgacaa agttttggta gcccggtgca
     2821 gcgttgacgt cagacgtacg gctaatgctg atacgatctt tcacatcgtt acggaaaacg
     2881 gtaacgctgg attcaacccc ttccagccag ccttcttcgc ccatgtagta aagccccagt
     2941 tcccaacttt cgctggtttc tggtttcaga tccgggctac ccacaatctt acatgcgcca
     3001 cggcaggaat tgctcgtcca gtcagggcta agttgcaaca gagaaggcgc tttaaatgcc
     3061 gtcgcccagc cccctttcac cgttacggtg tcggtggcgt tataaaccag gtaggcacgc
     3121 ggactccagt gttcaccgta ggtttcatga tcgtccatac gtacgccggt cgtcagcgcc
     3181 agcggctcga agatccgcca ttcatcttcc acaaacagcg cgtactggct ggcagacgtt
     3241 ttggagctgg ttcccccggt caggttcacc gcatcgctaa gtttgtcgtg acgccattca
     3301 ccgccaaccg tgagaaactg attaatcgcc gtcagcggca acgtgtattt gccgtcgacc
     3361 gtattgcttt cggaagttat cgggctgctg tagccagggt ttttgttctc gactttctca
     3421 ccgtagtatt tcagttcgct ggtgccgtaa tcccaacgcc cattatggct gacggagtag
     3481 ttctggcgtt ccaggcggtt tttgtccagc gagtcggaat cacgatcctg acggtcgaaa
     3541 ccgtatccgg cagtaaaatc gtgattttga ttcggtgtcc aggcaaattc gacattgccg
     3601 tcgcggctgg agaatccttc aatacgcggc gtttctccgg tatcggtggt cgttgagttt
     3661 tgcgggtcat ccttttcacg ttttgccagg ctgccgtaag ctttcattcc cagcacgccg
     3721 tcaattaatg gtccgctggt aaagaactga ccgttatagg tatcaccgcg atcgcgatgt
     3781 tcctgaacgg tggtatcgac ggtaacggtg cccgaccatt tctgaccgat ttttttggtg
     3841 atgatattca ctacaccgcc gagcgcatcg gaaccgtaca gcgacgacat cgggccacgg
     3901 accacttcaa tacgttcgat ggaatcgacc gggatccagt tcagatcgaa atcattgtgg
     3961 cggaagacgg cattgcggga gttaacgcgt ttaccgtcga caagaatcag ggtgtagctg
     4021 ctgtccagac cacgaatact tacgccctta cggttatccc cttcgttcgt cagttgtacg
     4081 ccaggcactt ctttgaggac atccttcaga ttctgtaccg gttttcgctg caggtcttcc
     4141 tgggtaatga cgctgatact ggcgggtgca tctttaaggt tttgctcaac ggaagatgca
     4201 gtgacaacca tcgtttcgcc atcatcatcg accgctaaca caggccatgc acaagaaata
     4261 gcggacaaac acagcccgac ccgtacgaaa gggttcaacc taaacattcc atatctccat
     4321 gaggtaacta cgaaaataaa atgggtatcg ctcacatctt ctttacgtcc ccttgcgtac
     4381 ggcagcatcg cggtggactt atttgtatgc aggtgatcat cccagaaagc cggaacagcc
     4441 tctgatttgt cagccttttt tgattgcggc gtaacgataa tgcaaacgat aacaattatc
     4501 aatccaaatg ttaaatttta tatccgcatg atttgtcagg gaaatggaat tgaaaaagcc
     4561 ctctcggttg agagggctta gcaaggaagg gaggaaactt atttcttatc gttctgcggg
     4621 aacttcattt cgctgtagcg tacgaagtga gttcctttaa tcagcttgta tccgaaccaa
     4681 ataatcagga acagcgggat accaatatac gttgccgcta cgccgcccca gtcaatggta
     4741 tctttcagga acgcttcgta gttctggccc aaagtgataa tcagacacag aatgaatgcg
     4801 aagatcggcc ccagtgggaa gaaacctgaa cggtacggca gatcgttaat gtcgtgtccc
     4861 tgcaatacgt acccgcgacg gaaacgatag tggctaatgg caatccccag ccaggcgata
     4921 aaaccggtca tcccggaggt gttcagcagc cacaggtata ccgtctggtt gccaaacatg
     4981 gaggtcagga agcacagacc tgcaatcacc gtcgtcgcat acagcgcatt acgcggcacg
     5041 ccaccacgcg acagtttagc gaaaatgcgc ggcgctttac cgtcacacgc cagggtgtac
     5101 agcatacgag tagacgcata cataccggag ttacccgctg acagcaccgc cgtcagaata
     5161 actgcgttca tcaccgccgc cgcagagagc agacccgcgt gctggaacac cagggtgaac
     5221 ggactgacgc tgatgtcttt aacatcgtta cgcagcaggc tcggatcggt atacggaata
     5281 atcaggctga taatcaggat cgcgaacaca tagaacaaca ggattcgcca gaatacctga
     5341 cgtaccgcgc gtggaatgtt tttcgccgga tcttcggact cgcctgcagc aataccgatc
     5401 agctcggttc cctggaaaga gaagccgaca atcatagcta cgccgatcat cgccgcaaaa
     5461 ccaccagcaa acggcgcttc gccgattgtc cagttgctcc agcccgcagg ctgcgcgcct
     5521 ttgaagatac cgataatcat cagcacgcca acgatgataa agacaataac tgtcgtgact
     5581 ttgatcagtg agaaccagta ttccgcttca ccaaagccac gaactgagat gtagttcagc
     5641 aggaagataa cgccgaggaa cagcgcactc cagatccagc ccggtgtatc cgggaaccac
     5701 cagctcatga ccagctgagc tgcaaccagg tcaacggcga tagtcaccgc ccagttgtac
     5761 cagtagttcc agcccagcgc gaagccaaag ccttcttcaa catagttctg accgtaagtg
     5821 gcaaacgaac cggaaaccgg catatatgca gccagttcac cgagactggt catcaggaag
     5881 taaaccatca ggccaatcag catatacgag agcaatgccc cgcccggacc tgcctgagaa
     5941 atcgttgcgc cagaggcaac aaaaagacct gtaccgatgg aaccgccaat ggcaatcatc
     6001 gtcaggtgac gcgcctttaa ttcacggcgt aagcccggcg cttctgtagt tttagtttcg
     6061 gaacccatac aaaaatgcta tccatcttaa aaatgaggcg cgattgtagc agacgattgg
     6121 caatccttcc ggcagaaata cacggttata agacaccttc atgatcgccc agggattata
     6181 agtaaagcag ccaatctctt ttctggcgaa tgcatgctga gggcgctgcg tcacgaaatc
     6241 gacacagcac cagcatgttc ttgtacagca ccttaacgcg gtacattcgc gggatcgcaa
     6301 tagtccagga agcgccgtag cgcgttggaa aggtgttttt gccgatgatg tatacgccac
     6361 aacgtacgca tcaggcgcgg aagagggacc gcaacttcac ttaatgtgcc tgcctgcaat
     6421 tgatcttcaa tcacccgtcg cgacaggcaa ctaataccca acccatgacg caccgcatgt
     6481 ttgattgcct cggagttacc taattccatc gccatctcaa acttcggtaa atgtgacagc
     6541 aacagataat cgacaatctc ccgcgtgccg gaaccgcgtt cacgcaggat ccacggcgca
     6601 gcggccagct gttctaaggt gaccggacca cgagccaacg gcgaagtcgg cgcggcgaaa
     6661 accaccagct cgtcttccag ccacggttca gaaatgattt cagtgctgtg gcacggtcct
     6721 tcaataaagc caatatcaac gcggaaatcc agcaccgctt gcatcacgtc ctggctattc
     6781 ccaacgctaa gttcaatcgg caactgcgga taatcatggc gataacgggc gataactgca
     6841 ggcagaatgt agttaccgat ggtactactg gcatagatac gaatcgcgcc gttgtcttcg
     6901 cgaaacagtt gttcgatttc aaccgcctgt tcaagcagtg ccaacgcgcg cggatagagc
     6961 agccgcccgt gttcattaac aaccagtctt ttccccacgc gatcaaacag ttgcacgcca
     7021 agctgccctt ccaggtcggt caaggctgcg ctcactgctg attgcgacaa cgccagcatc
     7081 accgacgcct gggtggttga tccacttttc aatacttctg caaaaacttc caactgccgg
     7141 agggtgatgt gcatagtcgc ttaccactta taaagattaa ttataaatat ataatcaatt
     7201 ttatttttaa accagttagt cgtaacctta tgcccggtta aaggagaggg ttatgacgaa
     7261 tatcacctta cagaaacaac atcgtacact gtggcatttt attccggggt tagccctgag
     7321 tgcagttatc accggggtcg ccctgtgggg tggttccatt cccgcggttg cgggtgccgg
     7381 gtttagtgcc ctcaccctcg caattttgtt ggggatggtt ttaggcaaca ccatctatcc
     7441 gcacatctgg aaaagctgtg acggtggcgt gctgtttgcc aagcaatatt tattacgtct
     7501 gggtatcatt ctttatggct tccgtctgac gttctcgcaa attgccgatg tcggtatcag
     7561 tgggatcatc attgacgtgt tgacgctgtc cagtaccttc ctgctggctt gcttcctggg
     7621 gcagaaagtg tttggtctgg ataagcacac gagctggttg atcggtgccg gtagcagtat
     7681 ctgtggtgct gccgcggtac tggcgactga gccggtagtg aaagcagaag ccagtaaagt
     7741 aaccgtggct gttgcaaccg ttgttatctt cgggaccgtc gcgattttcc tctacccggc
     7801 gatatatccg ctgatgtctc aatggtttag tccggaaact ttcggtatct acattggttc
     7861 tactgtgcac gaagtggcgc aggtggtggc ggcaggtcat gccatcagcc cggatgcaga
     7921 aaacgcagca gttatttcca aaatgctgcg cgtgatgatg ctggctccgt tcctcatcct
     7981 gctggcggcg cgtgttaaac agctgtctgg ggcgaacagc ggcgagaaaa gcaaaatcac
     8041 tattccgtgg tttgccatct tgttcatcgt agttgccatc tttaactcgt tccacctgtt
     8101 accgcagagc gtagtgaata tgctggtaac gctggatacc ttcctgctgg caatggcgat
     8161 ggcggcgctg ggtctgacca ctcacgtcag cgcgctgaaa aaagccgggg cgaaacctct
     8221 gctgatggct ctggtactgt ttgcctggct gattgttggt ggtggtgcga ttaactatgt
     8281 gattcaaagc gtcatcgcat aaaccactac atcttgctcc tgttaacccg ctatcattac
     8341 cgttttcctc cagcgggttt aacaggagtc ctcgcatgaa atacattgga gcgcacgtta
     8401 gtgctgctgg cggtctggca aatgccgcaa ttcgcgccgc cgaaatcgac gcaaccgcgt
     8461 ttgccttgtt caccaaaaac caacgtcagt ggcgtgccgc accgctcacg acgcaaacca
     8521 tcgatgaatt caaagccgcc tgtgaaaaat atcactacac atcggcgcaa attcttcccc
     8581 acgacagtta tctgattaac ctcggacatc cggtcactga agctctggaa aaatcgcgcg
     8641 atgcctttat agatgaaatg cagcgttgcg aacagctggg gctttctttg ctcaacttcc
     8701 accctggcag ccatctgatg cagatttcag aagaggattg ccttgcgcgt attgccgaat
     8761 ccatcaacat tgcgctggat aaaactcatg gtgtgacagc ggtgattgaa aacaccgccg
     8821 gtcagggcag taacttaggg tttaaattcg aacatctcgc ggcgattatc gacggcgtgg
     8881 aagataaatc ccgcgtcggc gtctgcattg atacctgcca tgctttcgct gccgggtatg
     8941 atttgcgtac ttctgccgaa tgcgagaaaa cgttcgcgga ttttgcccgt attgtcggct
     9001 ttaagtatct gcgcgggatg caccttaacg atgcgaaaag cacctttggc agccgcgttg
     9061 accgccatca tagcctcggt gaaggcaata tcggtcatga tgcgttccgc tggatcatgc
     9121 aggacgaccg tttcgacggc attccgctga tccttgaaac catcaacccg gatatctggg
     9181 cagaagagat cgcctggctg aaagcgcaac aaactgaaaa agcggtagcc tgaagatgaa
     9241 taaccgggag aaggagatcc ttgcaatatt aaggcgtaac ccgctgattc agcagaacga
     9301 aattgcggat atgctgcaaa tcagccgttc gcgtgttgca gcgcatatta tggatttaat
     9361 gcgcaaagga cggattaaag gcaaaggtta cattctcacc gagcaggaat actgcgtagt
     9421 ggtggggaca atcaatatgg atattcgcgg gatggcggat atccgttacc cgcaagcggc
     9481 ttctcatccc ggtaccattc attgctcggc aggcggcgtt ggacgcaata tcgcccacaa
     9541 tctggcgctg ttaggccgcg acgttcattt gctttcagtg attggcgatg acttttatgg
     9601 cgaaatgctc ctggaagaaa cgcgccgcgc cggcgtgaat gtctccggct gcgttcgttt
     9661 acatggtcaa agcacatcga cgtatctggc aattgccaat cgagacgatc aaaccgtgct
     9721 ggcgattaac gatacccatc tgctggatca gttgacaccg caactactga acgggtcgcg
     9781 cgatttactt cgtcatgcgg gcgtggtact ggcagattgt aacctgacag ccgaggcgct
     9841 ggaatgggtc tttaccctcg ctgatgaaat cccggtgttt gtcgataccg tttcagaatt
     9901 caaagcgggc aaaatcaaac actggctggc gcatattcac actctgaaac ccactttgtc
     9961 ggagctggaa attttatggg gccagccgat aacccgcgat gctgatcgta atgccgcagt
    10021 gaatgcgttg catcagcaag gcgttcagca actgtttgtt tatttgcccg atgagtctgt
    10081 ttattgcagc caaaaggatg gcgaacaatt tttgctgact gcgccagcgc atacgacggt
    10141 agacagtttt ggtgctgacg atggttttat ggcgggcctg gtgtatagct ttctggaagg
    10201 aagcagtttc cgtgacagcg cccgttttgc gatggcctgc gcggcaattt cacgcgccag
    10261 cggcagctta aacaacccta ccctgtctgc cgataacgcg ctttcattag tgccgatggt
    10321 gtaacaatct tgccggattc gacgcttgac cgcgtcttat ccggctcata actcgcgcac
    10381 tacgctaagc caataaagaa tcctgcaatg gtggcactca tcaggttaga aagtgtcgcc
    10441 gccgccagcg cgcgtaaacc aagctgggcg atttccggcg cgcggtgtgg cgcaaccgca
    10501 gaaaatgccc caaccactac cccgatagaa ccaaagttag cgaaaccgca caacgcgaag
    10561 gaaataatcg ccacggtttt agcatcgagc gtgccagctg tttgcagata aggtgagaaa
    10621 ttgaggtaag cgacaaattc attgatcgcc aacttctgcc caatcaaact cccggcaaga
    10681 tttgcatcac tccagtcaac ccccatcacc cacgccagcg gggccagcag ataacctaaa
    10741 atggactcca gcgaggcttg ctcaaaacca aaccagccgc caacgccgcc gataataccg
    10801 ttaattaacg caatgatggc aacaaatgcc atcaccactg ttgccacacc cgcagcgatt
    10861 ttcagcccgg tcattgcccc tgtcgcagcg gcctcaataa tactttttgg cggtgtttcg
    10921 gtgaaagaga ggttattaaa agaaacctgc gaagattccg tagccgggct taacaggcgg
    10981 gcaaacaaga tcccgcctgg gatcgccatt aacgatgccg ccagcaaata ttccacaggt
    11041 acgcccagtg cggcgtaacc aatcattgtc gaaccagcaa tcgaggccat gccactacaa
    11101 atcgctgtaa ataattcatt gcgattcagc cgatcgataa agggtttgac gattgccgga
    11161 atttcgtttt gtccgaggaa aatggtagtg accgcgacga atgactcgat cttgctgata
    11221 tttaatgctt tctggaagat accgccgaga atgcgaatta agatccccat cacaccgatg
    11281 tagtagagaa tactcaccag cgcggtgacg aagataattg ccggtaatac tctgaaacca
    11341 aagataaatc ctgcaccatc aaacagcgtg tccattttcg gcccgaccag cgaaccgaag
    11401 ataaatgcgc tacccgcgtc gctgtacgcc atcactttat ggacgccaaa agcgactttt
    11461 tcagcgaccc aacgccctgg cggtaaccaa agcataatgc cgccaatcac gacctgtaac
    11521 actaacgccg cgccaacggt acgcaggctg atcctcttct tgtttactga cagcaaaaat
    11581 gcaattgcca gcaataccac cattcccaga acacttctca tgacatccat agttattatc
    11641 tcttcatgtc attttgggcg acggctttct gcccatcaga aatcgccggg cctgcatctt
    11701 gcgatactgt cccggcttgt tatcaacact taatgggttt tgatgtagcc gcgcacgcac
    11761 tcttcgacta atccccagaa ccagtcggta tcaatagtga tgccgacttt ggtattggcg
    11821 ggcttgccaa gaacgcccag ctcgtcgcag acggtacgcc catagcaagg gccactgtta
    11881 acatccactt cgacgtacat ctcctgggtt ttaatgccat cggggttaat cagataaccg
    11941 atacaggtgg cgtcatgtac cgggccgcca gccagaccgt agttttcgaa ctgcgttttc
    12001 agagtgaagt tcatgatgtc gctgaacagc tctccggcgg ggccgcctgc cctttccatc
    12061 ctagcaatca cgtccggggt gcaaacggtc tggttggtga gatcgaggcc catcatcact
    12121 aatggaacgc cggaggtgaa caccactcgc gccgcttccg ggtccgcaaa gatgttgaac
    12181 tcggcagatg gcgtgaagtt gccagtacca taagcgccgc ccatcagtac aatttcacgg
    12241 atttttggca ggatcgcggg ctgcatacgc attgccaccg cgatatttga aagcggacca
    12301 accggcacca gagtgatatc gccatcgctt gccatcaggg tatcgatgat atatttcacc
    12361 gcatgagtgc tttctgcctg gcgggtcagc ggctcgaata ccgggccatc cagtccggtt
    12421 tcaccgtgaa tattatcggc aacgatttgt tgccgcataa tgggttgtgg catccccgca
    12481 taaaccggaa cattaatctc cagtttctgg caaacattca ggccattaat taatgtttta
    12541 tcaagcgtct gattacccgc tacaatggtg atacctaata aatcaatcgc cggatgtttc
    12601 gctgccatca tcattgcaat agcatcatca tgacccggat cacaatccag aataattttt
    12661 ctcttttcca ttgtttattt cctctgtttc cagttgcgtt attttttcta cagcaaagaa
    12721 aattcgaccg ggccgatgat tgaatcttaa caacagcgta cgtatgctaa atatgagaaa
    12781 tctcatagcg gataaatatc gtgaaagaaa tccacaataa tgatcttaag cagcaattga
    12841 tgagtgaatc cgcgtttaag gattgctttt caacggatgt ttcagccgat gcacggctgt
    12901 ttcatttttt agcacgtgac tacattgtgc aggaagggca acagccgtca tggctgtttt
    12961 acctgacgcg aggccgcgcc aggctttacg ccacgcttgc taatggtcgc gtgtcgctga
    13021 tcgatttctt tgccgccccc tgttttattg gtgagattga gttaatcgat aaagaccatg
    13081 aaccacgtgc ggtgcaggcg attgaagagt gttggtgcct tgcactcccc atgaaacatt
    13141 accgtccgct gttattaaac gacacgctat ttttacgaaa actctgcgtt agtttaagcc
    13201 ataaaaatta tcgcaatatt gtttctttaa ctcagaatca atcatttccg ttagtgaatc
    13261 gcctggcagc atttatatta ctctcacaag aaggtgatct ttatcacgaa aagcatacgc
    13321 aagcggcaga gtatttaggc gtttcttatc gacatctttt atatgttctc gcgcaattca
    13381 ttcacgacgg tttattaatt aaaagcaaga aaggatatct aattaaaaac agaaagcagt
    13441 tgtcaggact ggcgctggag atggacccgg agaataaatt ctccgggatg atgcagtaaa
    13501 aaataatctg caatagcgcg attgccggat gcaacgctta ataaggcgta catccggcaa
    13561 tgaaactcaa gctaaaccaa taaagaaccc ggcaatggtc gcgctcatca ggttggaaag
    13621 cgttgctgct gccagcgccc gtaaaccaag ctgagcgatt tccggtgcgc gttttggcga
    13681 aatagccgaa aatgccccaa cgacaacacc gatagaacca aagttagcaa aaccacaaag
    13741 cgcaaaggag ataatcgcaa tggttttcac ttccagcgtg ccgcccgttt gcaggtatgg
    13801 ggagaaactc aggtaagcga cgaattcgtt aattgccagt ttctgcccaa tcaggctacc
    13861 cgcaaggttg gcatcactcc agtccacacc catgatccac gccagcggtg ccagcacata
    13921 gccaaaaata ctttccagag aagcattggc gaagccaaac cagccgccaa ttccgccgat
    13981 aatgccgttg atcagcgcaa taattgccac aaataccatt accaccgtcg ccacaccagc
    14041 ggcgattttc agcccggtca tcgcaccgct cgccgccgct tcgataaagc tttttggcgg
    14101 cgtttcgctg aacgataaat tttcaaatgt gacctgcgaa ggctcggttg ccgggctaag
    14161 aatacgtgca aacaaaatcc cgccagggat cgccatcagc gatgccgcta acaggtagtc
    14221 aattggtacg cccattccgg cataaccaat catcatcgaa cccgcaatgg acgccatccc
    14281 gctacaaatt gcggtaaaca actcgttgcg attcatgcga tcgataaacg gtttaacgat
    14341 cgccgggatc tcattttgcc cgaggaaaat agtggtaacc gcaacaaaag attcgatttt
    14401 gctgatgttg agggctttct ggaaaatgct gccaaggatg cgaatcagca gccccatcac
    14461 gccaatgtag tacagcagac taatgagcgc agtgacgaag ataatcgccg ggagtacgcg
    14521 aaaggcgaag ataaaacccg caccgtcaaa gaggacatcc attttcggcc caaccagcga
    14581 accaaaaatg aaggcgctac cggcatcact gtaagacatc actttatgaa cgcctaatgc
    14641 cgcctgttct accgcccatt ttcctggtgg aaaatagagc atgattccgc caatggcgat
    14701 ttgtagcagc aacgcggctc caaccgtgcg caaactgatg ctctttttat tcactgacaa
    14761 caaaaatgct attgccagta acaccaccat ccccacaaca cttctcatta tatccataat
    14821 gattttccct tcatgccagt aaacccggcg tcagcgccag gttttggtat gtatgatcag
    14881 tacgggcgac ggctttctgc ccgtcagaaa ttaacccgcg agacgctggt attctttggc
    14941 aatttcgctc gccagaatgg cgttgttgaa caccagttgg atgttggatt tcaggctgtc
    15001 accgccagtc agttcagcaa cacgagccag caggaacggt gtgctttctt taccaataac
    15061 gccctgctct tcggcttcgg cgacggcttg atctatggct gcattgatag attcctccgg
    15121 catcgcaaac tgttccggga tcggatttgc caccaccagg ccaccgttca gaccgctttg
    15181 ccatttcacc gccattgcac gagcgatttc cgtggcacta tcaaggcgaa tactaacttc
    15241 aaatgggctg gtacggcaga aaaacgcagg cagcgctttt gtctgatagc caattaacgg
    15301 cacaccgaag gtttctaaat attctgttgt taatcctaaa tcgagaatag attttgcacc
    15361 ggcacaaaca acggtgacat tagtatttgc cagttcttgc aaatcggcag aaatatcgaa
    15421 ggtatgttcc gccccgcgat gcacaccacc aattcccccg gtggcaaata ctttaattcc
    15481 ggcaagcgcc gcaataatca tcgttgaagc cacagtggtt gcgccatttt ttccggcggc
    15541 aacgacaaaa ggtaaatcgc gacgactaac tttggtcacg ttatgccctt cgcgacccag
    15601 caattcaatt tcttctttgc ttaaacccac tttcatcacg ccgccaataa tggcgatagt
    15661 ggcaggtaca gcgccctgtt tacgaatagt ttcttcaact tcaattgcgg tctgggcatt
    15721 ttgtgggaac ggcatcccgt gagaaataat agtcgattcc agcgccacaa ccggtttttt
    15781 gttttttaaa gcgtcctgca cttccgggga gatttgtaat aattcagggg aaatttttaa
    15841 ttcagacatt ctacgttctc cactaacgat ataacgttgg caatcgataa atcggggtta
    15901 ttggtgtatt cacaggagag cgccattgac gaacatccct gtgcgaaacg aacagattcg
    15961 gcaaacggca ttccgtctac ccaacacgaa gcaagtcccg ccatcatggc atcgcccgct
    16021 ccggtaacat taatgacatt ggttttgatc ggcgcagacc aaccactttc accgctgata
    16081 tcactgtaat aaacgccgtc gccgcccatg ctcaatacca gtcggttcag gccatgttga
    16141 tggaaccagg cggcaacctt tgccacatct tcacgccctg acagcgcaat cccactcagg
    16201 gtttccgctt caaggcggtt tggcttgaga gtgtggattt gatttagacg gtcgcgcact
    16261 ttgacacatt tccacgctga aaccggatcg acaaatacgg gtacgttggc ggcattatcc
    16321 agaatccatg ccagtgcctc ttcactgata ttacagtccg cgacaatgac ctttgccctc
    16381 tgaataaatt cacggtgctg tgcgagatat tcagctgtaa tagcgttgct aatattcatg
    16441 tcatttatag caaccagcat ttcaccggta ttatcgagta atgataaata gctcgacgta
    16501 ttttctcccg gcacaatcag gcatttatcg acataaacgc cagattgatt ggtttgcgtt
    16561 agcagcgatt ggccataaaa atcactgcct acggcgctca gtagccaggc tttattaccc
    16621 agcaacgcca ggttttgtgc aatattgcgc cctaccccac caggcgtaaa ttttatttta
    16681 cctggatttg aatccgcata atttaatgat tcatgtgaat atccggcgac atcaatattc
    16741 gccgaaccta taattacgac ataatccttt tcgcgcatag cacgtcctct ggtaaaaaat
    16801 taccaaccct gtcaatccac aagaatattg atactgcaaa gacggtttaa acatgtgttc
    16861 atatttaaac atgtgctcat agtatgcatg ttttacgtaa agtaaattac cgccagagag
    16921 gcaaaatggg cagatttgag caggctcaca aaacgcgatt aaatgcctga attataaaaa
    16981 cggggaattt gtgacgggat gcacagaaca ggaacactaa aaaagggcag aaattaatct
    17041 gcccttgagg aaataacctt agtgtttgat gaaagtttca ggtttccact aaaagataaa
    17101 accatttaaa ttcataaaat tacattcaca aaccggtcat tccctttgcg ctcatcaaat
    17161 gttacagggc aggaaatttc tgccctgtaa cacacctttt attacgctgc tttcgctact
    17221 gcgtccactt ccggacgttt caggaaggca taggccaacc ccgccaccag cgtaccggca
    17281 ataatggcta ccaggtaacc caataccggc gtaatcgcgc cagggatcag cagaacaaac
    17341 agaccaccgt gcggtgccat cagtttcgca ccaatcgcca ttgagattgc gccagtcagc
    17401 gccccaccca cgatacagca cggcagcaca cgcatcggat cacgggcagc aaacggaatt
    17461 gcaccttccg aaataaagca cagtcccagt accagagcag ctttgccccc ttcctgctgc
    17521 gccttgtcga atttgcgacg cgccaccatt gttgccaggc ccattgccag cggtggcacc
    17581 atacctgccg ccataatcgc cgccatcggg ccataggttt gagtactcag cagacccaca
    17641 ccgaatgcgt acgctgcttt gtttaccgga ccgcccatgt cagtacacat catgccaccg
    17701 aggatcgccc ccagcagaac cgcattcgca gtccccatgg tctgcagcca gtgagtcaac
    17761 ccttcgagaa tgccagcaac cggtttaccg atcaggtaga tcatcgccag accgaccacc
    17821 agactggaaa ttagcggaat gatcagaatc ggtttcagcg cctccatact ctgtggcagt
    17881 ttcaattgcg tactgattaa cttcgcaatg taaccagcca ggaagcccgc aataataccg
    17941 ccaatgaagc cagaaccggt gctgaccgcc agcataccgc caatcagacc cggcgtgaga
    18001 cccggacgat cggcaatgga aaaggcaata taacctgcca gtaccggcac catcaacgca
    18061 aaggctgaac caccaccaat ctgcatcagc gccgcagcca acgtacccgg ctctttaaac
    18121 gcttcgatac caaaagcaaa agaaagcgcg atacacagac cacctgcaac gaccatcggc
    18181 agcatgtaag agacgcccgt cagcaagtga cggtaagcgc ctgcactctc tttcttacct
    18241 tcagtggtcg ctgtttgagc tttgcccgct ggttcatacg gcgttgcttc agcaaccgct
    18301 ttatccagtt cctgcgcagt tttcttcagc gccagaccgg tagaggtgcg atacatcggt
    18361 ttaccagcaa atttcgccag atccacttcg atatctgccg ccacaatcac cagatccgct
    18421 gctgcgactt cttcaggggt gattgcatta cccgcgccaa cagaaccacg ggtttcaact
    18481 ttcacccacc agccacgttt tttcgcttcg gtttcaatgg cttcagccgc cataaaggtg
    18541 tgtgctacgc cagtcgggca agcagtcacc gcaactacgc gtttcggacc gctggcggca
    18601 accggtgctg tcgcagcgac cggcgcagtg taaggtttcg catggccttt ggcttcactc
    18661 aggaacagct cagggtgcgc aactgcccgg gaaatatcgc ccagccagac atttttaccg
    18721 ttcagcgcgc tgtcattcgg gatggaatca ccgagaacaa tcgccatttc tgcgtcgttc
    18781 ggattgtcga tgatttccag ttttgctttt cgcgccgccg cgcccagcag ggtcttcgcc
    18841 atataggcgc gtgcctgacc gagattagcg tcaataatca gcagcgtttt cattatgcct
    18901 ctcctgctgt cagttaaaag gttgtaagtc gacgcgcgcc atcattgcgg ccaactgcgg
    18961 acgatcggta atacccacat tgctttgact taccgccagg gctgcaacag ctgtcgccag
    19021 acgcagtgtg tgttcactgg attcacgcat cagcaagcca taaatcaggc caccaaccat
    19081 agaatcccct gcgccaacgg tgcttacgac atcgactgac ggtggtttgg cgatccattc
    19141 gccggaggca ttaacccaaa gcgcgccttc ggcacccagt gaaataacga catgcgcgat
    19201 accttgttca cgcagcgcat gcgcagcttc aatcacatct ttcatttcag gcagtttacg
    19261 gcccgcccag atttccagct cgcggcggtt aggtttcacc agccacggtg ccgctttcaa
    19321 acctgctact aacgcttcac ggctactatc aaagataatg caaggacact gactacgcag
    19381 gcgagtcatc cagtcggtga acgcttccgg gctgacgcct gacggtaagc ttccgctgac
    19441 acagaccata tcgaactgac cgagccagct cagagaatca gtcacaaagc gttcccagtc
    19501 ggcgggggtg acttcaaaac ccgagaagtt gaagtcggtc acttcgccgt ctttttccgt
    19561 cagcttaacg ttaattcggg tgcgcccctg tacaacctgg aaacggttgg caatgcccag
    19621 ctcgctgaac agttgctgaa aaccatcctg attgtcttta cccaggaagc cgccaacggt
    19681 gacatcaatt cccaggtctt ttaatacttt ggccacgttg atgcctttac ccgccgcatg
    19741 cagaccggtg gttttcacca ggttcacttc gccgcgttca atttccgggc agaaaccaac
    19801 aaggtcataa gccggattaa gggtgatcgt agcaacacgt ctgctcatta tgcgccctcc
    19861 ccaagaccag cagcgatagc gtcgccaatg gctttcagcg cctgttcagc atctgcaccc
    19921 tgggcggtaa agcgtaggcg atgacctttc ttaacgccaa gtgccacaac tttcatcaga
    19981 ctacgtccgt ttgccggttt gccggtgcca tcaaggtttg tcacggtaat atcactgtta
    20041 aattgtttaa tggtattgac cagcatggta cctggacgag catgcaggcc gtgttcattg
    20101 cgcaccacaa actccgcgct taacacgtcg tcggtcggcg catcatcgct ggtcagcagc
    20161 gccagcaacg ttgccgcatc cgctttcagc aagcggtcag ctttattatc gagcaacaaa
    20221 tcagcgagac gcttaagaac cgcaatgggc tgatcgtcat tcatcgccac actcaccagc
    20281 atggctgccg tttcgccgtc cacatcaaaa gcatttgccg cacggcttac cgcaatcgcg
    20341 ctacgcagat tgccttcagc gctatcgctc agccagatgc cctgtccgag gttcagcggt
    20401 tgttcattga tggctttagt gacgaaggtg gcgtcaactg cccctgcttc tttcagacgc
    20461 gcagcgttaa gcgcctgaag cgtcagcaga tcgctggcga caatatccag cgtcagcatt
    20521 tcgttatcga gcttcagctg ctcactctgc ttttcgccca tcagcaatgc gcgaagttct
    20581 tctgctgtcg ttgctgactt cagctgttca gcaacggaat catcgcttag tacgtgggtc
    20641 agctggcgta acagacccag atgttcatcc gagctggcag caataccgat cgccacgtac
    20701 gctacctggc cgtcacccca ggtgacgcct tccgggaact gaaatacctg aacgccggtt
    20761 ttcagcacct gatcgcgggt gtcggtagtg ccgtgtggaa tagcaatacc attgccgagg
    20821 aacgtagagg tttgctgttc gcgcgccagc atgtcattga cgtagccttc agctacatta
    20881 ccggcctgca ccagcgccgc agcgacctgg cgaatcgcct cttctttgtc tccggccttt
    20941 tcgcccggat ggatgtcctg tacggataac tggaacatag ttctcctctc ttgctgaatt
    21001 gaaacgattc agcttctatg agaaaaaaag cgccaacctg gcttagggtt aaagacaaga
    21061 tcgcgctgaa acgtttcaag aaagcataat acttctgttt cagcacgcaa ggaaagccgc
    21121 aaactaatga gcaaaagttt gatgtgctgc acaatttttt gtcatttttc ctaattgctg
    21181 atgggaaaat ccgttatcag caatttcatt tcagcagcgt cagcagccaa tcagcaacag
    21241 tgaaatggca ttttgattat tcgtggcgct tttgatttcg ctgtctgttt attttctaac
    21301 aagcagcgta aactccgcgt cttcctcttc cagtgatcga ccagcatgca taactccccc
    21361 gcagtctcca gcgcgaaatc gtttgatctg acctcgacgg cgtttttaat cgttgccttt
    21421 ctcaccggta ttgcgggcgc tttgcaaacc ccgacactca gtattttcct taccgatgaa
    21481 gtacatgccc gtccggcgat ggtgggattc ttctttaccg gcagcgctgt cattgggatt
    21541 ctggtcagtc agtttctcgc cgggcgctct gataagcgcg gcgatcgcaa atcgctgatt
    21601 gtcttttgct gcctgttagg cgtgctggcc tgcacccttt ttgcctggaa tcgcaactac
    21661 tttgttttgt tattcgttgg cgtctttctt agcagctttg gctcgaccgc taacccgcaa
    21721 atgtttgccc ttgcacgtga acatgccgac aaaaccggac gtgaggcggt gatgttcagc
    21781 tcttttttac gcgctcaggt ttcactggca tgggtcattg gcccgccgct ggcttatgcc
    21841 ttagcgatgg gtttcagctt tacggtaatg tatctgagcg cagcggtagc gtttattgtt
    21901 tgcggcgtga tggtgtggct gtttttaccg tcgatgcaaa aagagcttcc gctggcgacc
    21961 ggcacggttg aagcgccgcg ccgtaaccgt cgcgatacgc tgctgctgtt tgtcatttgt
    22021 acattgatgt ggggctcgaa cagcctgtac atcatcaaca tgccgctatt tattatcaac
    22081 gaactgcatc ttcccgagaa actggcaggc gtgatgatgg ggaccgccgc cgggctggaa
    22141 atcccgacca tgttgattgc cggatatttt gccaaacgtc tgggtaagcg tttcttaatg
    22201 cgcgttgctg ccgtgggtgg cgtctgtttt tacgcaggaa tgctgatggc gcattcacct
    22261 gccattctgt tgggcttgca gctgctaaat gctattttta ttggcattct gggcggcatc
    22321 gggatgcttt attttcagga tttgatgccc ggtcaggcag gttcagccac cacgctctat
    22381 accaacacgt cgcgcgtggg ctggatcatc gcaggatctg tggcgggcat cgtcgccgag
    22441 atctggaatt atcacgctgt gttctggttt gcgatggtga tgattatcgc cactctgttt
    22501 tgcttactgc ggattaaaga tgtttaaggc gcggtcagca tttccagatc gataagccag
    22561 gtcattgcct gctgacgcga gttaccgcac atttcagcgc tggcttgtaa gcctgcacag
    22621 actttcggcc gcagtggcga ggtgaaaatt ttacagcgtt gctgttcatc aagctgaatg
    22681 cagggcgtat tggcgggctt gccatctggc ataccgggaa tagggctgga aattgaaggg
    22741 gcagtgcaac acgccccgca acccggacgg cattccatac gttgttctct taaggttaac
    22801 caccggattg atgagcgcgc acagtaccac cttttttgca ccagcaaaag tgcgaatacc
    22861 acttgcccga aaggcccgtc gcgagtactt tgtcgcgata tttttgacat tttcgactac
    22921 aggaattttt cgatgccaag agcgaacgaa attaaaaaag gtatggtact gaattacaac
    22981 ggcaaactgc tgctggttaa ggatattgat attcagtcgc ccactgcccg cggtgccgct
    23041 acgctgtaca aaatgcgttt ttctgatgtc cgtaccgggc tgaaagtaga agagcgtttc
    23101 aaaggtgatg atatcgttga caccgtgacg ctgacccgcc gttacgttga cttctcctat
    23161 gtcgatggca acgaatatgt ctttatggat aaagaagact ataccccgta taccttcacc
    23221 aaagatcaga ttgaagaaga gttgctgttt atgcctgagg gcggcatgcc ggacatgcag
    23281 gtgctgacct gggacggtca actgctggcg cttgagctac cgcagaccgt tgatctggaa
    23341 atcgttgaaa ccgcacctgg catcaaaggg gcatctgcca gcgcccgtaa caaaccggcg
    23401 acattgagca ctggtctggt gattcaggta ccagaatact taagcccggg cgaaaaaatt
    23461 cgtatccata tcgaagaacg ccgttatatg ggtcgtgctg actaacttca gccgcatgca
    23521 gaaaagggat agctcaggct gtcccttttt taatttatta taccaatctt ctattttgcg
    23581 cttcctgaac accttacgcc cgcaattttc actcgttgat tgatcgccct cacacttcat
    23641 cgcattaaca atccagacca atttcaattg ctgtcatata actttacact gtcattgtta
    23701 attaatcgtt actaagacgt gactcctatg aatacaatcg cctccgttac gctcccgcat
    23761 catgtacacg ctccacgcta cgatcgccag cagttgcaat cacgtatcgt tcattttggc
    23821 tttggagcct ttcaccgcgc tcatcaggcg ttactgaccg atcgtgtgct gaatgcccag
    23881 ggcggcgact gggggatctg tgagatcagc ttgttcagcg gcgatcaact gatgagccag
    23941 ctccgcgcac agaaccattt atataccgtg ctggagaaag gtgcggacgg caatcaggcg
    24001 ataattgtcg gtgccgttca cgactgcctg aatgcaaaac tggattcctt agcggcaatt
    24061 attgagaaat tttgcgagcc acaggtggca attgtttccc tgacgattac cgaaaaaggc
    24121 tattgtattg acccggccac cggtgcactc gacaccagca atccgcggat tattcacgat
    24181 cttcaaaatc ctgaagaacc tcactccgcg ccgggtattc tcgtcgaagc actgaaacga
    24241 cgccgtgaac gcggccttgc accgtttacc gtactctcct gcgacaatat tcccgacaat
    24301 ggccatttgg tgaaaaacgc ggtgctgggg atggcggaaa aacgttcacc agaactcgcc
    24361 gggtggataa aagaacacgt cagttttccg ggaaccatgg tcgaccgtat tgttccggca
    24421 gcaaccgacg aatcactggc ggaaatcagc cagcatctgg gggtgaatga tccctgtgcg
    24481 attagctgcg aaccgtttat ccagtgggtg gtggaagata acttcgtcgc tgggcgtcct
    24541 gcctgggaag tcgcaggtgt acaaatggtg aatgatgtcc tgccatggga agagatgaaa
    24601 ctgcgtatgc ttaatggcag ccactctttt ctcgcttatc tgggttacct ctcaggattc
    24661 gcccatatca gtgattgcat gcaggatcgc gcatttcgcc atgccgccag aacattaatg
    24721 ctggatgagc aagcgccgac actgcggatt aaagatttcg atttaacaca atatgcggat
    24781 aagttaattg cacgttttgc taacccggcg ctgaaacata agacctggca aatcgcgatg
    24841 gatggcagcc agaaattacc gcaacgtatg ctggcaggta ttcgcataca tctggcgcgc
    24901 gaaacggact ggtcgttgct ggcattaggc gttgcaggct ggatgcgtta cgtcagcggc
    24961 gttgatgatg ccggaaatgc cattgatgtt cgcgatccgc ttagcgataa aatttgcgaa
    25021 cttgttgcgg gcagtaacag tgaacaacgc gtaaccgccc tgctttctct gcgtgaagtt
    25081 ttcagtgatg atctgccaga taacccgcat tttgtgcagg caatcgaaca agcctggcaa
    25141 caaatcgcac aattcggcgc gcatcaagcg ctattaaaca ccctcaaaat ttaacgattt
    25201 ctgcggttaa agcggatgaa gctcgccttc gtccgctctc cccttctctt ttctgccttt
    25261 tttagccagg attaacgctc agttaacttt taagagtatt gcggagccgt tgtgaccaga
    25321 accaacctca tcaccggttt tctcggcagc gggaaaacta cgtcgattct tcatctgtta
    25381 gcccataaag atcccaacga aaaatgggcg gtactggtta atgaatttgg ggaagtcgga
    25441 attgatggtg ctttgctcgc cgatagcggc gcattgttga aagagatccc cggcggctgt
    25501 atgtgctgcg ttaatggttt acccatgcag gtagggttga ataccttact gcgtcaggga
    25561 aaaccagacc gcttgttgat agagccgacc gggctgggcc atccgaaaca gatcctcgat
    25621 ctcttaaccg cgcccgtgta tgaaccgtgg atagatctgc gcgccacgtt gtgcattctc
    25681 gatccacgcc tgctgctgga cgaaaaaagc gccagcaatg aaaacttccg tgaccagctg
    25741 gctgccgcag acattattgt cgccaataaa tccgaccgtg cgacgcccga aagtgagcaa
    25801 gcgctacagc gctggtggca gcaaaatggt ggcgatcggc aattaattca cagcgcgcat
    25861 gggaaagttg acggtcatct tctggatttg ccgcgtcgca atttagccga gttgcccgcc
    25921 agcgccgcgc attctcatca gcatgtcgtg aaaaaagggt tagcagcgtt aagcctgcca
    25981 gagcatcaac gctggcgtcg cagtctgaac agcgggcaag gatatcaggc ctgcggctgg
    26041 atattcgacg ctgatacggt attcgacacc attggcattc tggaatgggc gcgacttgca
    26101 ccggtagaac gcgtcaaagg cgtgctgcgt attcccgaag ggctggtgcg aatcaaccgt
    26161 cagggcgatg acctgcacat tgaaacgcaa aacgttgcgc caccggacag ccgtattgag
    26221 ctgatttcca gcagcgaagc tgactggaat gccttgcaga gcgcgctgtt gaagcttcgt
    26281 ttagcgacta ccgcgtaagg ttgcctgcgt ttttcagtaa gataattaga gaaaatatga
    26341 ttaaaaattt gccgcaaata gtgttgttga atattgtcgg cctcgcgctg tttctttcct
    26401 ggtatattcc cgttaatcat ggattctggt tgccgattga tgcggatatt ttttatttct
    26461 ttaatcagaa actggtcgaa agtaaggcct ttttgtggct ggttgcattg accaacaatc
    26521 gcgccttcga cggttgttca ctgctggcga tgggtatgtt gatgctgagt ttctggctga
    26581 aagaaaacgc ccctggcaga cgacgtatcg tgattattgg tctggtcatg ctattaactg
    26641 cagtagtatt aaaccagctg ggtcaggcat taattcctgt aaaacgggcc agcccaacat
    26701 tgacttttac cgatattaac cgcgtcagcg aactgctctc tgttcccacg aaagatgcct
    26761 cacgagatag ctttcccggc gatcacggta tgatgctgct tattttttcg gcattcatgt
    26821 ggcgttattt cggcaaagtt gcaggcctta tcgcccttat tatttttgtg gtttttgcat
    26881 ttcccagagt aatgattggc gcacactggt ttactgacat cattgttggt tcgatgaccg
    26941 tgatattgat cggtttgccc tgggtgttgc tgacgccatt aagtgatcga ttaatcacct
    27001 tttttgacaa atcactaccg ggaaaaaaca aacatttcca aaacaaataa ctcacagtaa
    27061 ttaacatcat cagggttatt tttatagtga ggataatcct gatgatgcgc accgtgcttt
    27121 catctatcga acgcaaaaat cattctctaa gtaaatgaat gtattgcatg cgtttcactc
    27181 aatcgtactt taattgacca atcccgctta ttaactttct gtatcacttt ttcttataaa
    27241 aaatcatgta aaaccgctcg ccaagaccgc accaatcggg taatctcgaa ctcgttttgc
    27301 ctcggcggta gattatcctc acagcatata attttgtgcg ttagtccaca gatttggcct
    27361 taaggaattg tttcaacatg cccaggtaat tagtctcgtg tcgcttggca tttttttata
    27421 acgatatttg tcgttaagga cttcaaggga aaacaaacaa catggtcaaa tctcaaccga
    27481 ttttgagata tatcttgcgc gggatccccg cgattgcagt agcggttctg ctttctgcat
    27541 gtagtgcaaa taacaccgca aagaatatgc atcctgagac acgtgcagtg ggtagtgaaa
    27601 catcatcact gcaagcttct caggatgaat ttgaaaacct ggttcgtaat gtcgacgtaa
    27661 aatcgcgaat tatggatcag tatgctgact ggaaaggcgt acgctatcgt ctgggcggca
    27721 gcactaaaaa aggcatcgat tgttctggtt tcgtacagcg tacattccgt gagcaatttg
    27781 gcttagaact tccgcgttcg acttatgaac agcaggaaat gggtaaatct gtttcccgca
    27841 gtaatttgcg tacgggtgat ttagttctgt tccgtgccgg ttcaacggga cgccatgtcg
    27901 gtatttatat cggcaacaac cagtttgtcc atgcttccac cagcagtggc gttattattt
    27961 ccagcatgaa tgaaccatac tggaagaagc gttacaacga agcacgccgg gttctcagcc
    28021 gcagctaata aaccgtttgg atgcaatccc ttggctatcc tgacgagtta actgaaagca
    28081 ctgcttaggc agtgcttttt tgttttcatt catcagagaa aatgatgttt ccgcgtcttg
    28141 atccaggcta tagtccggtc attgttatct tttaaatgtt gtcgtaattt caggaaatta
    28201 acggaatcat gttcatacgc gctcccaatt ctggacgtaa gctcctgctt acctgcattg
    28261 ttgcaggcgt gatgattgcg atactggtga gctgccttca gtttttagtg gcctggcata
    28321 agcacgaagt caaatacgac acactgatta ccgacgtaca aaagtatctc gatacctatt
    28381 ttgccgacct gaaatccact actgaccggc ttcagccgct gaccttagat acctgccagc
    28441 aggctaaccc cgaactgacc gctcgcgcgg cgtttagcat gaatgtccgt acgtttgtgc
    28501 tggtgaaaga taaaaaaaca ttctgttcat ctgcgactgg tgagatggac attccactaa
    28561 aagaattgat tccggcgctc gacattaata aaaatgtcga tatggcgatc ttacccggta
    28621 cgccgatggt gccgaacaaa cccgcaatcg tcatctggta tcgcaaccct ttgctgaaaa
    28681 atagcggcgt ctttgccgct ctgaatctca acctgacgcc ttcactcttt tatagttcac
    28741 ggcaggaaga ttacgatggc ctcgccctca ttattggtaa tactgcgcta tctacctttt
    28801 cttcacgttt gatgaatgtt aatgaattaa ccgacatgcc ggtccgtgaa actaaaattg
    28861 cgggcattcc tctgaccgtt cggctttatg cggatgactg gacatggaac gatgtgtggt
    28921 acgcattttt actgggtggc atgagtggaa ctttcgttgg acttctctgc tattacctga
    28981 tgagtgtgcg tatgcgccca ggcagagaaa tcatgaccgc catcaagcgc gaacaatttt
    29041 acgtggtata tcaaccggtg gttgatacac aagctttgcg ggtaacgggc ctggaagtac
    29101 tgctacgctg gcggcatcca gtagcaggag aaatcccccc ggatgccttc attaactttg
    29161 ccgaagcgca aaagatgatt gtgccactga ctcagcacct gtttgagttg attgcccgcg
    29221 atgccgcaga attagaaaaa gtactgccgg taggcgtcaa atttggcatt aacattgcgc
    29281 cggcccactt gcacagcgaa agctttaaag cggatatcca gaaactgctc acttccctgc
    29341 ccgcacacca tttccagatt gtgctggaaa ttaccgagcg cgatatgctg aaagagcgag
    29401 aagccacaca actcttcgcc tggctgcatt cggtcggcgt agaaattgct attgatgact
    29461 tcggcaccgg gcacagcgcg cttatctatc ttgagcgttt tacgctcgat tatctgaaaa
    29521 ttgatcgtgg atttatcaac gccatcggta cggaaacgat cacttcaccc gtacttgacg
    29581 cggtgctgac gctggcgaaa cgtctcaata tgctgacagt tgctgaaggg gtcgaaacgc
    29641 cagaacaggc acgatggcta agcgaacgcg gcgttaattt catgcaaggc tactggatta
    29701 gtcgcccgtt accgctggac gattttgttc gctggctgaa gaaaccgtat acgccgcagt
    29761 ggtaaggtgt gcttacgtcc cttattattc atagtgaaag catgccggat tgcggctaat
    29821 gatgagtaaa aggaaatccg ttgcagatga ttgtgcgcat actgctgctg tttatcgctc
    29881 tgttcacctt tggtgcgcag gcgcaggcta tcaaggaaag ctatgccttt gccgtactgg
    29941 gcgaaccccg gtacgcattt aatttcaacc attttgatta tgtgaacccc gccgcgccaa
    30001 aaggtgggca aataacgttg tccgccctcg gcaccttcga taatttcaac cgctatgcac
    30061 tacgcggcaa cccgggcgca cgcaccgagc agctgtacga cacgctattt acgacttccg
    30121 atgacgaacc aggcagttat tacccgctga ttgctgaaag cgcacgctat gctgacgatt
    30181 attcctgggt ggaggtcgct attaatccgc gcgcccgttt tcatgatggt tcgcccatta
    30241 ctgcccgcga tgtagagttt acttttcaaa aatttatgac cgaaggcgtg ccgcaatttc
    30301 gtctggtcta caaaggcacc accgtcaaag ccattgcgcc gttaaccgtg cgcattgagt
    30361 tagctaaacc cggcaaagaa gatatgctga gtctgttttc gctgccggta tttccagaaa
    30421 agtactggaa agatcacaaa cttagcgacc cgctcgccac gcctccgctt gccagtggtc
    30481 cgtaccgcat tacgtcctgg aaaatggggc aaaatattgt ctattcccgc gtaaaagatt
    30541 actgggcagc aaacttaccg gtaaaccgtg gacgctggaa tttcgacacc attcgctacg
    30601 attattacct cgatgataat gtcgcctttg aagcgtttaa agcaggtgcc tttgatttgc
    30661 gtatggaaaa cgacgccaaa aactgggcca cgcgctatac cggtaaaaat ttcgataaaa
    30721 aatacatcat caaagatgag caaaagaacg aatcagccca ggatacgcgc tggctggcgt
    30781 ttaatatcca acgtccggta ttcagcgatc gccgggtgcg ggaagcgatc actctcgcct
    30841 ttgactttga atggatgaac aaagcgttgt tttacaatgc ctggagtcgc acgaacagtt
    30901 attttcagaa taccgaatac gcggccagaa attaccccga cgccgcggag ctggtgcttc
    30961 tggcaccaat gaaaaaagat ctaccgccag aagtcttcac gcaaatctac cagccgccgg
    31021 tatccaaagg cgatggctac gatcgtgaca acctgttaaa agccgacaaa cttcttaacg
    31081 aagcaggctg ggtgctgaag ggtcagcaac gcgttaatgc cacaacgggt cagccactca
    31141 gctttgaatt attgcttccc tcaagcagta atagtcagtg ggtattgccg ttccagcaca
    31201 gcctgcaacg tctgggtatc aacatggata ttcgcaaggt ggataactct caaatcacca
    31261 accgcatgcg cagtcgcgac tatgacatga tgccgcgcgt atggcgggcg atgccgtggc
    31321 ccagttccga tttacagatt tcctggtcat cggaatatat caattccact tataatgccc
    31381 ccggcgtgca aagcccggtt atcgactcgc tgatcaacca aattattgcc gcgcagggaa
    31441 ataaagaaaa attactgccg ttggggcgag cactggatcg cgtattaacg tggaattatt
    31501 acatgctgcc aatgtggtat atggcggaag accgtctcgc ctggtgggat aaattctccc
    31561 accccgctgt acgccctgtt tacagcctgg gtatcgatac ctggtggtat gacgttaaca
    31621 aagcgacgaa actgccgtca gccagacaac agggagagta gatgggcgct tatctgattc
    31681 gccgtctgtt gctggtgatc ccaacgctat gggcgattat cactatcaac tttttcatcg
    31741 tgcaaattgc gcctggcggt ccggtcgacc aggccatcgc cgccattgag tttggcaatg
    31801 ccggagtatt acccggcgca ggcggtgaag gtgttcgcgc cagccatgcg caaacgggcg
    31861 tcggcaatat cagcgacagt aattaccgtg gcggacgcgg attagatcca gaagtgatcg
    31921 ccgagatcac tcatcgctac ggtttcgata agccgatcca cgaacgttac ttcaaaatgc
    31981 tctgggacta catccgtttt gattttggcg acagcctttt tcgcagcgcc tcggtgctga
    32041 cgctgattaa agacagtctg ccggtttcca tcaccctcgg attgtggagc acgctgatta
    32101 tctatctggt gtcgattccg ttaggcattc gcaaagctgt ttataatggg agccgctttg
    32161 acgtctggag tagcgcattt atcatcatcg gctacgccat tccggccttt ttgtttgcca
    32221 tcctgctgat tgtcttcttc gcgggcggca gctatttcga cctgttccct ctgcgcggcc
    32281 tggtttccgc taactttgat tcgctgccgt ggtatcagaa aatcaccgat tatctgtggc
    32341 atatcacgct gccggtgctg gcgactgtga ttggtggctt tgcagcgctg accatgctga
    32401 caaaaaactc attccttgat gaagtgcgca agcaatacgt ggtgaccgcg cgcgcgaaag
    32461 gggtaagtga aaaaaatatt ctctggaaac atgtgttccg caacgccatg ctgctggtga
    32521 ttgccggttt tccggcgacg tttatcagca tgttttttac cggctcgctg ctgattgagg
    32581 tgatgttttc actcaatggt cttggtttac tgggctacga agcgaccgtc tcgcgcgatt
    32641 atcctgtaat gtttggtacc ttgtatattt tcaccctgat tggcctgctg ctgaatattg
    32701 tcagtgatat cagctatacg ctggtcgatc cgcgtataga ttttgaggga cgctaatgcc
    32761 gcgactcagc cccgtcaatc aggcccgttg ggcgcgtttt cgccataacc gtcgcggcta
    32821 ctggtcgtta tggattttcc tcgtcttgtt tggtttgagt ttgtgttctg aacttatcgc
    32881 caacgataaa ccgttgctgg tacgttatga cggcagttgg tatttcccgt tgttgaaaaa
    32941 ctacagcgaa agcgattttg gtggcccgct ggcaagtcag gctgattatc aggacccgtg
    33001 gctgaaacaa cggctggaaa ataacggctg ggtactgtgg gcaccgattc gctttggtgc
    33061 taccagtatc aactttgcta ccgataagcc cttccctgct ccaccctccc ggcaaaactg
    33121 gctgggaacg gatgccaatg gcggcgatgt gctggcgcgc attctctatg gcacgcggat
    33181 ctcagttctg tttggcctga tgctgaccct ctgctccagc gtgatgggcg tgttggcggg
    33241 ggcgctacaa ggctattacg gcggtaaagt cgatctctgg gggcaacgtt ttattgaagt
    33301 atggtcgggg atgccgacgc tgtttttgat tattttactt tccagcgtcg tacagcctaa
    33361 cttctggtgg ctgttggcaa ttacggtctt gtttggctgg atgagtctgg tcggcgtggt
    33421 gcgggcggag tttttacgta cccgtaattt cgactacatc cgtgcggcgc aggcgcttgg
    33481 cgtcagcgat cgcagtatca tcctgcgtca tatgttgcca aatgccatgg tcgctaccct
    33541 caccttttta ccgtttattt tatgtagttc gatcaccacc ctgacctcac tcgatttcct
    33601 cggcttcggt ctgccgctcg gttcaccgtc actcggcgaa ctgctgttac aagggaaaaa
    33661 taaccttcag gccccgtggc ttgggatcac cgccttcttg tcggtggcga tattgttgtc
    33721 tttgctgatc tttattggtg aagccgtccg cgacgcattt gatcctaata aggcggtgta
    33781 gcatgacgca aactctgtta gcgattgaaa atttgtcggt gggttttcgc catcagcaaa
    33841 ccgtacgtac agtagtcaat gatgtttcac ttcagattga ggctggcgaa acgctggcgc
    33901 tggtgggtga gtcaggttca ggcaaaagcg ttaccgcgct gtcaatttta cgcctgctcc
    33961 cttccccgcc ggttgaatat ctctccggcg atattcgttt tcatggcgaa tcgctgcttc
    34021 atgccagcga tcaaacgtta cgcggtgtac gcggtaataa gattgccatg atttttcagg
    34081 agccgatggt gtcattaaat ccattgcata ccctggaaaa acagctttat gaagtgcttt
    34141 cactccaccg cgggatgcgt cgggaagcgg ctcgtggcga aattcttaac tgccttgatc
    34201 gcgtcggtat ccgccaggcg gcaaaacggc taacagatta tccgcatcag ctctccggcg
    34261 gcgaacgcca gcgggtgatg attgcgatgg cgctgttaac gcgaccggaa ttattaattg
    34321 ccgatgaacc gaccaccgcg ctggacgtct ctgtccaggc gcagatttta cagctgttgc
    34381 gcgaactgca aggcgagctg aatatgggca tgctgtttat tactcataac ctcagcattg
    34441 tcagaaaact ggcccaccgc gtggcggtaa tgcaaaacgg tcgctgtgtc gagcaaaata
    34501 acgctgctac gctatttgcc tcacccactc atccttacac acaaaagcta ctcaacagtg
    34561 aaccatctgg cgacccggtg ccattgccag aacctgcctc tacgttgctg gatgttgaac
    34621 agcttcaggt tgccttcccc attcgcaaag ggattttgaa gcgcattgtg gatcataatg
    34681 tggtggtgaa aaacatcagt tttacgctac gggcgggtga aacactgggt ttagtgggcg
    34741 agtccggttc cgggaaaagc acgacgggac tggcgctgct gcgactgatt aattctcatg
    34801 gcagcatcgt ctttgacggt cagccactgc aaaatttaaa tcgccgccag ctgttaccta
    34861 ttcgtcatcg cattcaggtg gtatttcagg atccaaactc ctcactcaac ccacgactca
    34921 acgttttgca aattattgag gaaggcttac gggttcacca gccgacgctt tctgccgcac
    34981 aacgcgaaca acaagtgata gccgtgatgc atgaagtggg attagatcct gaaacacgcc
    35041 accgttatcc ggcggagttc tctggtggtc agcggcaacg tattgcgatt gccagggcgt
    35101 taattcttaa gccctcgctg atcattcttg atgaaccaac atcatcactc gacaaaacgg
    35161 tgcaggcgca aatattgacg ctattgaaat cattgcaaca aaagcatcaa ctggcctatt
    35221 tgtttatcag tcacgatttg cacgttgtcc gcgcgttatg tcatcaggtt atcgtactgc
    35281 gacaagggga agtagtggaa caaggaccgt gcgcgcgcgt gtttgccgca ccgcagcagg
    35341 agtatacgcg tcagctactg gcgttgagct gacgcttaaa aaggattgta gtttgagaag
    35401 ggttcggcaa ttgccacacc aaaatttttc aatcggcatg ttgcagcaaa ctcgtcctgg
    35461 cgatttacaa acaggcacgg ctcaccttca cactccagta ccgagcatgg aacttcgatg
    35521 tcgcttaacg cctggcttag tttgtacatc accgaccatg cattatcacc atccgggcta
    35581 agcaatttaa gcgccaccag gctgttgtca ccgcacggtc cattttgcgg aatcggttca
    35641 accttcgaac ctgcgaaacc agcagaccag cgatagttct gcggcagtcg atggacgatt
    35701 gagagttgta atgaagtcac ttattttccc cggaagcata ttttaattca caatttattt
    35761 acatcaattt taacacatca tcaacaatcc gtctttatac agatcgtaaa gattgatgcc
    35821 tcgcctgcgt ggcctctatg gctctgattt aaataatatc tgcgggcgtg gtcacgtttg
    35881 cggggctata tgtacccgtt tctgcgatct aactcaacct ttttaactac aatgatgtga
    35941 ctttttacat aaattgattt tacataaaat aaacatatat cgggggaatg atagatttgt
    36001 ggttgacatg catcaacaaa ggaagccttt tagcttcctc gttgtgcaat agatcaccgt
    36061 tttttcggcc gactggcgta cagacagaag agaatggaac tggttgcgca gaatgcaatt
    36121 gaccaaatca tcggccatgc agagttaaag gtcgcaagag aaagtaatgc gccaacaatt
    36181 gcccctatgc cgaaacggaa ggttcctgcc agcgaagatg ccgttcccgc catatgggga
    36241 aactcatcaa gaatgaccgc catcgcattg gatgacacca tcgacacgca gcccacaaac
    36301 gccgcaacgc caaccaccag cgaccagaat cccagcccca gcagcgcact gatgaccatc
    36361 cacgccgcca taataaactg tatccacaac cccgagcgga acatatttaa cgcgccaata
    36421 cggcgaacga agcggctgtt aaagatagtc atcacgaaca gaaaaacaat gtttagcgca
    36481 aagtaataac caaagttttc cggcgcgacg tggttaattt caatgtagac aaacggtccg
    36541 gcgcttaaga acgagaacat cccggcaaag ctgaaaccac tggcaagcat ataactcagg
    36601 acacgcttat gacggaacag cgccgcaaag ttaccaatag tagtacgaat gtgaaaaggc
    36661 tggcgacgct ccggtggtaa ggtttctttg atcagaaaga agatcattgc cgaagccaga
    36721 atcgccgcca cagcgaggat ccagaagatg taatgccagc tcagccacac cagcacccag
    36781 ccgccaacta tcggtgccat cagcggtgca atggtcgtca ccagcatgac aaacgacatc
    36841 atccgcgaga actcttcttt cgggtagata tcacgcatca aagcgttgat aaccacgctg
    36901 gccgcagccg cagccagccc gtggaagaaa cgcatcacaa tcagctgatc gatggtttgt
    36961 gccaacgcac acgccaccgc ggcggcggca aacaccagcg taccgccaag aaccaccggc
    37021 ttacgcccga agctgtctgc catcggcccg tagattaact gccccaacgc aaagcccaga
    37081 atataagtac tgagggtcat ctgcgtactg cccgccggta caccaaactg cgctgaaatt
    37141 accggtagcg caggcagata catatcaatc gacagcggca tcaacatggc cagcaggcca
    37201 aggataaaaa caatagcaaa cgacgaatgc tgtcgggtgg tcacaacggg ctcctgaaag
    37261 tcattgaaaa gttagacgac gctggcaatt tcttcttcag ttaacggacg atattcaccg
    37321 ggggctaaat cagcatccag cgtaatgccg ccaatacgtt cacgatgcag ctcaaccaca
    37381 tggttaccca cggcggcgaa catgcgtttc acctgatgat aacgcccttc gctgatggtc
    37441 agacgaacct gcgttggggt aatcacttcc agcaccgccg gcttagtgag atctttttcg
    37501 ttatgcagtt gcacgccttt agcaaattgc tctgccgtat cgtcagctac gggtgattcc
    37561 agcgtcacca gataggtctt ctcgcaatga tggcgcgggg aagtaatgcg gtgcgaccac
    37621 tgaccatcat cagtcattag taccagaccg gtggtatcga tatccagccg ccctgcggcg
    37681 tgcagtttcc acgccactgg ttcatcaaga aaatagagca ccgttgggtg atcagggtcg
    37741 tccgtggagc aaacatagcc ctgaggctta ttgagcatga agtaacgtgg accgtgttgc
    37801 tgcgccagcg gattgccatc gtaagcgaca tcatgttcag ggagcagttt gaacgctgca
    37861 ttacggacga tttcgccatc gacggtgaca cgattgccgc ggatttcacg cccggcaata
    37921 gcacggctaa cgccgagttg ctgtgcgata aatttatcaa gtcgcatgtg tgtgattttg
    37981 cctgtaaaaa acggaggtcg ggtaatgacc cgaaaatctg aactgttgtc tgcccagtat
    38041 agcggtctaa taacttccct caagggaaaa agatccgtgg catactatta gcagaataat
    38101 ctacctacgc gagaccatga tttttacact ccgcccatat cagcaagaag ccgtggatgc
    38161 cacgctcaac cattttcgtc gtcataaaac ccctgccgta atcgtgctgc ccaccggtgc
    38221 aggtaaaagc ctggtgatag cggaactggc gcggctggca cgtggtcgcg tgctggtgct
    38281 ggcacacgtt aaagaactgg tggcgcaaaa ccatgcaaaa tatcaggcgc tggggctgga
    38341 agccgatatt tttgccgccg ggctaaagcg caaagagagc cacggtaaag tggtatttgg
    38401 cagcgtgcag tcggtcgccc gtaatcttga tgcctttcag ggtgaatttt cgctgttgat
    38461 tgtcgatgaa tgtcaccgta ttggtgacga tgaagagagc cagtatcagc aaatcctcac
    38521 tcacctgacc aaagtgaatc cccacttacg cctgctgggg ctgactgcca cgccttttcg
    38581 actgggcaaa ggctggattt atcagttcca ttatcacggc atggtacgcg gcgatgagaa
    38641 agcccttttc cgtgactgca tttatgagct gccgctgcgt tatatgatta aacacggcta
    38701 tctgacgccg ccagaacgac tggatatgcc agtagtgcaa tacgatttca gccgcttgca
    38761 ggcacagagt aacgggctgt tcagcgaagc cgatctcaac cgtgagctga aaaaacaaca
    38821 acgtattacc ccgcacatca tcaaccagat tatggagttt gctgcaacgc gcaaaggggt
    38881 gatgattttc gccgccaccg ttgaacacgc aaaagagatt gtgggattac tacccaccga
    38941 agatgctgca ctgattactg gcgacacccc cggcgctgag cgcgatgtgt taattgaaga
    39001 ttttaaagcc cagcgttttc gctatctggt caacgtcgcg gtactgacca ccggatttga
    39061 cgccccgcac gtcgatctta tcgccattct gcgccctacc gaatcggtga gtctttacca
    39121 acaaattgtc gggcgaggtc tgcgtctcgc tcctggcaag actgattgct taattcttga
    39181 ttatgcgggt aatcctcacg atctctacgc gccggaagtt ggtacaccga aaggcaaaag
    39241 tgacaacgtt ccggtacagg ttttctgccc tgcctgcggt tttgccaaca ccttttgggg
    39301 gaaaacgacc gccgacggga cattgattga acactttggt cgccgctgtc agggatggtt
    39361 tgaagatgac gacggtcatc gcgaacagtg tgacttccgt ttccgtttta aaaattgccc
    39421 gcaatgtaac gcagaaaatg atattgccgc ccgccgttgc cgcgagtgtg acaccattct
    39481 ggttgacccg gatgatatgt taaaagcggc gctacgactg aaagacgcgc tggtattacg
    39541 ctgtagcggc atgtctttgc agcatgggca cgacgaaaaa ggcgaatggt tgaaaatcac
    39601 ctattacgat gaagacggcg cggatgtgag tgagcgtttc cgtctgcaaa cgcccgccca
    39661 gcgaactgcc ttcgagcagc tttttatccg cccgcatacg cgcacaccgg gcatcccgct
    39721 gcgctggatc accgccgccg atatcctcgc ccagcaagcc ttattgcgac acccggattt
    39781 tgtcgttgcc cgcatgaaag gtcagtactg gcaagtgcgt gaaaaagtgt tcgattacga
    39841 aggtcgtttt cgtcgggcgc acgaattacg cggttaatgg tagttttgat tgatgtataa
    39901 ggcgattgag tatagaatct cgcccgcttt tgcatacgca aagcagatca cttacctgtt
    39961 gctgggtcgc ctgtagcagg attaatttaa gagagaaaga aatgtttact atcaacgcag
    40021 aagtacgtaa agagcagggt aagggtgcga gccgccgcct gcgtgccgct aacaagttcc
    40081 cggcaatcat ctacggtggc aaagaagcgc cgctggctgt cgaactggat cacgacaaag
    40141 tcatgaacat gcaagttaaa gctgaattct acagcgaagt tctgaccatc gttgttgacg
    40201 gtaaagaaat caaagttaaa gctcaggacg tacagcgtca cccgtacaaa ccgaagctgc
    40261 tgcacatcga cttcgttcgc gcttaattgc tgaataagtt gtaaaaaacc ccgctccggc
    40321 ggggtttttt gtctctgcaa attatgcctg attacggtat tgctattttt tgcaggccag
    40381 ataaggcgtt cacgccgcat ctggcattca gaacacaaaa ccgattaatt gccgccagac
    40441 gtccggcgct gcaattggtc acgcaggttc ggcggcgtgc ctttaatggt cagggtatcg
    40501 gtcgccggat cccagaagat gcgttcacct aacagcatcg catcaaagtt aatagtcaag
    40561 ccaccgccgc taccggcaaa cttcgtcaac tgacgcagtg tgctacggtc tgccgggaag
    40621 ctctcttcca gttcgtagcc tttttcagcc gcaaactccg tgaagctcac ttcgctgacg
    40681 ccagccagct ctttcgacag cgattccagt tcaatctctt cacctgcctg cagctgctca
    40741 ttacagtagc tataaacctg ctggcgcacg ttctgtcgct ctgctttatc cagttgtgct
    40801 tcggcagtga aatcatcgac cgcttgcaac agcccacggt tttgcgcttt cgcgtttaac
    40861 ccttcgctgg caccgaggaa atccataaag aaatccgcca ctttgcgccc tactcgccct
    40921 ttcaggaaag tgagatagcg ggtcgactct ggattggttt cccactcggt taaatcgata
    40981 cgcgcaacaa tatccgcatg attgatatca agataatgag ttgggttgat gtcgagattt
    41041 tcgttaacgc gcatactgct cagattgctc agaactgcca ccagcaaata ctccaccgcc
    41101 agatagcgat agtggcaaaa cagcacgaat ccaccgtcag caaaaggata tttcgctaat
    41161 tcgtcacgca ggcgaccggt tgccgcgcgg ctaaatgcca gaaagtcctc ttcgccctga
    41221 cgctgtaagc gcagcgtctg cgccagttca ctctcttcgc taaacaagcc gtaagcttta
    41281 tttttggcgc tatagacccg atgcagttct gccaccatct caacgacggt ttctgtcggt
    41341 tccagcaatg aatcgcgcaa caccagctca aggttctgct catcacgctt gataagctgg
    41401 tgcagagcaa tctggttgat atccagactc atgataaact ctcctttaag accgggcggt
    41461 attcaaccac cgccggttaa gcgacgcaat aaaagatggc ggtcatttta cagaaggcag
    41521 cgtaacgaga atgatttaac gcataaaaaa gcagaaaaaa aaccgttgct acggtaatat
    41581 gttgcccttt catcaactaa ctgatttcga tttatgccac aaatttcccg ctacagtgat
    41641 gaacaggttg aacaattgct cgcagagctg ctcaacatac tggaaaaaca taaggctccg
    41701 accgaccttt ccctgatggt gttaggcaac atggtcacta accttatcaa caccagcatt
    41761 gccccggccc aacgccaggc aattgccaac tcttttgccc gcgccttaca gtcctctatc
    41821 aacgaagaca aagcgcacta agggaaacag ataacaggtt atggtaactc atcgtcagcg
    41881 ctaccgtgaa aaagtctccc agatggtcag ttgggggcac tggtttgcac tgttcaatat
    41941 tctgctttcg ctcgtcattg gcagccgtta cctgtttatc gccgactggc cgacaacgct
    42001 tgctggtcgc atttattcct acgtaagcat tatcggtcat ttcagcttcc tggtgttcgc
    42061 cacctacttg ctgatcctct tcccgctgac ctttatcgtc ggctcccaga ggctgatgag
    42121 gtttttgtcc gtcattctgg caacggcggg aatgacgcta ttactgatcg atagcgaagt
    42181 ctttactcgt ttccatctcc atcttaatcc catcgtctgg caactggtta tcaacccaga
    42241 cgaaaatgag atggcgcgcg actggcagct gatgttcatc agcgtgccgg ttattttatt
    42301 gcttgaactg gtgtttgcga cgtggagctg gcaaaagctg cgcagcctga cgcgtcgtcg
    42361 acgcttcgcg cgcccgctgg ccgcattctt atttatcgcc tttatcgcct cgcatgtggt
    42421 gtatatctgg gccgatgcca acttctatcg cccgatcacc atgcaacgcg ctaacctgcc
    42481 gctttcgtac ccgatgacgg cgcgacgttt tcttgagaag catggtctgc ttgatgcgca
    42541 ggagtatcaa cgccgtctca ttgagcaagg taatcctgac gccgtttccg ttcagtatcc
    42601 gttaagcgaa ctgcgctatc gcgatatggg caccgggcag aatgtgctgt tgattactgt
    42661 cgatggcctg aactactcac gcttcgagaa gcagatgcct gcgctggcag gttttgctga
    42721 gcaaaatatt tcgttcacgc gccatatgag ctccggcaac actacagaca acggcatctt
    42781 tggcctgttc tatggcatct cgccgagcta tatggatggc attctgtcga cccgtacgcc
    42841 tgcggcatta attactgcgc ttaatcaaca aggctatcag ctgggattat tctcatcaga
    42901 tggctttacc agcccgctgt atcgccaggc attgttgtca gatttctcga tgccgagcat
    42961 acgcacccaa tccgacgagc agaccgccac gcagtggatc aactggctgg gccgctacgc
    43021 acaagaagat aaccgctggt tctcgtgggt ttctttcaat ggcactaaca ttgacgacag
    43081 caatcagcag gcatttgcac ggaaatatag ccgggcggca ggcaatgtcg acgaccagat
    43141 caaccgcgtg ctcaatgcac tgcgtgattc tggcaaactg gacaatacgg tggtgattat
    43201 cactgccggt cggggtattc cgctgagcga agaggaagaa acctttgact ggtcccacgg
    43261 tcatctgcag gtgccattag tgattcactg gccaggcacg ccggcgcagc gtattaatgc
    43321 tctgactgat cataccgatc tgatgacgac gctgatgcaa cgcctgctac atgtcagcac
    43381 acctgccagc gaatattcgc aaggtcagga tttgttcaac cctcaacgcc gtcattactg
    43441 ggttaccgcc gcggataacg atacgctggc aattaccacc ccgaaaaaga cgctggtgct
    43501 gaacaataac ggtaaatacc gtacttacaa cttacgtggt gaaagagtga aagatgaaaa
    43561 accacagtta agtttgttat tgcaagtact gacagacgag aagcgtttta tcgctaactg
    43621 attaattata aatcagttag caaaatatct tacttgcaat cggtgtggaa aacggtagta
    43681 ttagcagcca cgagtcggca cgtagcgcag cctggtagcg caccgtcatg gggtgtcggg
    43741 ggtcggaggt tcaaatcctc tcgtgccgac caaaaatccc aagaaaaaac caaccattac
    43801 ggttggtttt tttatatctg caattaattc gataaacaga ccgtgacaca tcacagcctg
    43861 tttattttct gttatcagaa cgtccagacc acacccgcct gagtattcca cggcgattct
    43921 acccctgcgc cattgccata actgactgac aaatgaccgc ttaacgtcgg ggtaaacgat
    43981 gaccttatcc cagcctgata aacgccacgg gttccagcca catcattatt aaatttgcca
    44041 tcgtcattaa ctttcacctg gttagaatcg gcgtattcct gacgtacggc ggctttcagc
    44101 cagggttcca gcgtcgtacc gttttgcagg tccatgtgat agcttaccgc cgttcccgct
    44161 tcagcccgta atatgcgggt atttcccaca tccgcgcgca tgccgtttga taacgtgtag
    44221 tcctgaccat ctgtggtaaa gccggtaaag gccagataag gtctaacact ccacaatccg
    44281 tcaacccaac ggaacccgct ctcgacatga gcacccgcgc cgttactatt gtaatcgcca
    44341 aacgctgttg ccccattact catcttgcca tggatggtgt tggcaaaacg gtcaactttc
    44401 accaccccat caacataggc accgttctga tgctcccaac cggcataagc ccccagggta
    44461 tagctatcga tattgccttt gccgccgcga tcaaaaccaa tatcagaatg agagtaacca
    44521 aagaacaagc cgcgaattgt actgctttct tcacgggaga aacggctatc gataccgagc
    44581 gtcagccccg tcaatgtttg ctcaaagcca gctcccgcat cagtggtcac gttgttgcgg
    44641 gtgttaattg ccgaactcca catcgccgta tcgtaattaa cgccttttac gctaccaaga
    44701 cgctcacgca cggtgtccag ttctgcatca aataccagcg gttgtgctgc cgccatattc
    44761 agcacatcag tggttgaagg ggtaatttgc gcgcgattct ctgccagact ccagctatgg
    44821 ttgccattat ccagcaaggt atattcatac gtaccgatat caacaacgcc tccggcattg
    44881 cccaacgtaa atgcagcatc accgccgccc gtcgttacca gtgtaaggct atctcctgct
    44941 gccgggctgg caccggtgtc cgtaacgaat attttgaaat cacctgttgc ctgaccggtg
    45001 acgttgagct gatcgctctg atgattagcc atatcggtac gcatataaaa attgccgttt
    45061 cccgacaggg tattggtggt caacgtaatg aaattttccg cacttgccgt cactgatgaa
    45121 cgagtcactg gctcatccat cataatcaca tttgcgtcag acatcgcaag gttggctaag
    45181 ttgaggaggg tgttttggtt cgtcgtagtg atatcggcaa tgatggtcca tgagctacca
    45241 tcgagcgaaa catccgcttt gctggtatcc acggcaccat gcgttcttaa agaagcgccc
    45301 tcagaaacca cgacactacc caccaacata gcgggtgcat aatttatttg cggcttcatg
    45361 acctcaagaa tgccatcatt ccctctgact gaaacgttaa ccattgtgcc agcccagaca
    45421 ttagcgcggc cattgttgat aaccatattt tcagagatgg ctttagagga atacgtgata
    45481 cttccattct gatctgatcg accgagatca tataccgcat ttgcctgcac tctcgtacca
    45541 gtatcctttc ccagcgattg catagtggca tgctcatcaa ggatagtatc aatggcctgc
    45601 gtgtcctcca taacaatgag cccggaacca tcatccagtt cataattttt tgacacacca
    45661 tcttttatac taaattggcc tttactgttt gtaccactca cttccagcgc attcgttgag
    45721 acaattaatt ttccgcctgc ttttttatca acgccagttg cagtcccgcc atccataacc
    45781 tccagtaagc cgccattatc cacagtggta ttataagcga agtcattttc ttcaactcgc
    45841 aaacttccgc cgttttccaa cagcatatta ttagcaatac cattcttgat atcgaactga
    45901 ccgagacggt ttgttccgaa tacctccatg gcccgcgtgg ttgttttaat cgcaccgcct
    45961 gctttctgat ctaccgcaag cgcaaatccc ccctctttaa cctccagtag tcctcccttt
    46021 tcgatagcgg caaagacggt ccccccatct ttttcaacga ttaagcgccc gcctttaatc
    46081 gtaggttcat tcgccgcgcc ataaacctgt agcacaccat tattgagggt agtctggttt
    46141 gccgcgccac ccatttgaac aatttgataa ccatcattta atactgagta ttctgcggtg
    46201 ccattcattt caatgtactg atacccgccg ttaatttcag tattactact tataccaaat
    46261 tctttaatat gctgttctcc gccaggattg attttcgtat tattggcggt cccataaacc
    46321 agctggatat ctttttccag gacaacacca tcaactgtct caccatactc aactgttgat
    46381 gccaatgcta tgggtgaata taacgctgta gccaccagaa cagatattgc ggaacgacaa
    46441 agagaaacag aaccagattg atgcattgag ctttcatcct atgaaattaa ttgctgttaa
    46501 aagcattggg taaagaaaat acccatagct ccatacccgg agtcagtttt taaaaactgt
    46561 ttaaagaaat gcacaagtat tgtgattgat tttttagttg tttttcttga tgagaagctg
    46621 atgcaaaatt ccgtctttat aatgaaaatg atgccaaagc gaacgacaag gttgtagttt
    46681 tcactacatg tccatacata aaatggggta acattcacgc gcctggtagc gttaccaacg
    46741 ctacgctcaa acataatgat tctaataaaa cctcaggaga ctactatgcc tgaagcaacg
    46801 ccttttcagg tgatgattgt ggatgatcat ccacttatgc gacgcggtgt tcgtcagtta
    46861 ctggagcttg atcctggctt tgaagtggtc gccgaagcgg gcgacggcgc gagcgcgatc
    46921 gatctggcga ataggctgga tatcgacgtg atcttactgg atctcaatat gaaaggtatg
    46981 agtggcctgg ataccctcaa tgccttgcgc agggatggcg ttaccgcgca aattattatc
    47041 ctgaccgtat cagatgcctc cagcgatgtc tttgcgctga tagacgcagg cgcagacggt
    47101 tatctgttga aagacagcga cccggaagtg ttgctggaag cgattcgtgc cggagcgaaa
    47161 ggcagcaaag tctttagcga acgcgtcaat cagtacttac gtgaacgtga aatgtttggc
    47221 gcagaagaag atcccttcag cgtgctgacg gagcgcgagc tggatgttct gcacgagctg
    47281 gcacaggggc tgtcaaataa acagattgcc tcggtgttga atatttccga gcagacagta
    47341 aaagtacaca ttcgcaatct gctgcgtaaa ctcaatgtcc gctcacgcgt ggcggccacc
    47401 attctgttcc tgcaacaacg cggggcacaa taaaaatagc ccgatggcat caccatcggg
    47461 cctcttttta tttactctcc tgcggcgaca aatgttgcat cgcctgcgcg atactacgtt
    47521 caatcaccgc acggcgagta tcattggcag gtaagagttt caacatcatc tcccacgcgg
    47581 caacggcttc gccaaatcgc tgctgctcaa aggcattaaa cgcatacatg cttaacacac
    47641 ggatattgct gtggtcagtt ctcaccaact ggcgtagcag ttcaccaccc aggcggttgt
    47701 cgttgggatc agatgaacgt gtcaacgctt cagcgtatcc gagcgccgca tcactgttct
    47761 tcggatcgag gcgatatgca gtagcgtatg catcggtggc aatactggcg ttacccagcg
    47821 ccatgccaac gcggcccaac ataatccagc cttctatatc tcccggattt ttttgcagtt
    47881 gagtacgcat ccccagcgcc aggcgcgaca tctcttcttc gttgagcgga tcggctttcg
    47941 gatccagcgc cctgtccagt aacgccggag cctgtgccgt ggcctgctgc cagattttca
    48001 cctgctgata attgcccgtc tggtagtagc tgacgccagc cacaattaac gccaccacaa
    48061 tacccggcag ataaacaata tatccggcac gcttaccttc cggcacgctt tgttcaggaa
    48121 acgcgtccgg caccacgcgt acccgacgcc gcgaacgggc gtaaatgacc caaccgccaa
    48181 tgccaatagc cactaccgga agcacccaca gcagcacggt cagcggcgtt aacggcggat
    48241 cgtaagtgac gaagttgccg taacgcgcca ccatataatc gacaatctct ttcttacttt
    48301 taccttcctg catcagttca tacactttct ggcgcaggtc ggtggcaatc atcgagttgg
    48361 aatcggcaat gctgttgttc tggcatttcg ggcagcgtag ttcttcagtg agttggcgga
    48421 actgctgctc ctgcgcttca tccttaaact gcaacacgtc gatggtcgcc agcgctgagc
    48481 cggagatcat cagcatcagc acgcccaata aaaacctcat tgtgcggcct ccttactgta
    48541 tttctcccac agcggcttga tctcttcttc ccagacgcga ggattgagat cgcccgcatg
    48601 gcgatagcga atgatgccgt tgccgtcgat gaggaacgtt tctggcgcgc catagacacc
    48661 gagatccagc cctaacatgc cgtcgccatc aaacaggctt agtgcgtaag gattgcccag
    48721 ctctttcagc cagctgattg ccttctggcg atcgtcttta tagttcatgc cgaccacgcg
    48781 aatgccctgc gcagaaagct gattcagata ttgatgttcc gcacggcagg tcggacacca
    48841 ggtcgcccag acgttaagca gcacaggttt gccctgagtc agcacatccg cctgataaaa
    48901 ctgccccgga ttgtccagtg attcgaggcg aaacttcggc actggcttgc caatgagcgc
    48961 cgattccaga ttggtcggat catccccttc ggcattacgc gccagctgcc acagcagcgc
    49021 cgcggcaatc gccaggaaga taatcaacgg aattaacaat actttgcgct tcatacagcc
    49081 tccggcgcag ttttttgcgg actcacgcgc ttacgatagc gaggatcaaa cagacacagc
    49141 agtccgccca acgccatcat cagcccgccc gcccaaatcc agcgaacaaa cggtttgtag
    49201 taaagacgca cggcccacgc gccgttttcc agctcttcac cgagcgcggc gtacaggtca
    49261 cgcgtgatgc cgccgtcaat cgccgcttcg gtcatcatcg acccggcagt gttgtaataa
    49321 cgtttttccg catacagcac cgtttccggc ttgccatcgc gcgttacgcc gatagtcgcc
    49381 acaccgccac gccagttcgg gccagtcacc tctttgacat cacggaaggt gaagcgatat
    49441 tcatggatat cgacactatc gccggacttc atgcgcacat cacgctcaac gctatagttc
    49501 tggctaaagg caataccaac aattgtcact gccagcccca ggtgagccgc caccattccc
    49561 caataactga aggtggtttt cgtgccgcgt gaaatacgca gcgcagcttc cgcaattgcc
    49621 agcaccgcaa tccagcaggc cattgccagg ccgagcaccg tcatcgccac cactttgctt
    49681 tcgaacagcc acggcaacag cagcgacagc accagcgtag agatgaaggc gataatcaac
    49741 aaattgcgga ttttacgtgg gcgatcgcgc ccccagcgca ccagaggacc gacaccaagc
    49801 agcagcgcaa acggcaccat cagccaggta aacatagtgt tgaagaacgg ttcgccaatc
    49861 gaaatactgc ccagtcccag ttgcttgtgc accagcggca gcagcgtccc cagcaacacc
    49921 accagcatcg cggcgaccag caaaacgttg ttcgctaaca gcagagattc ccgcgaccac
    49981 agcgcattgt ttacgcgtga gcgaactttg tgtccacgcg cggcaaacag cagcagcgaa
    50041 ccaccaatca ccagtaccat aaaggcgagg ataaacatac cgcgtgccgg atcagaggca
    50101 aacgcgtgta ccgataccag cacgccggaa cgcaccagga aagtccccag cagacacaac
    50161 gagaaggcac tgattgccag cagtaatgtc cacgctttga agctggcgcg ttgttcagtg
    50221 accgccagtg agtgcatcag cgcagtcccc accagccacg gcataaacga ggcgttttct
    50281 accggatccc agaaccacca gccgccccag ccgagttcgt aataggccca tgcggaaccg
    50341 agcacaatgc cgagcgtcaa gaagatccac gccgccagcg tccacggacg agtaaaacgc
    50401 gcataagtgc tgtccagacg cccgctcaac aaagaagcaa tggcaaaagc aaacgccacc
    50461 gagaagccga cgtaccccat ataaagcaga ggcggatgga agatcagccc cggatcctgt
    50521 aacagcgggt taagatcgcg accttcaatc gggaagttcg gcaacgtgcg tgagaacggg
    50581 ttagaggtaa agagaatgaa cagcaaaaag ccgacgctga ccatccccat tatcgccagt
    50641 acgcgggcca caatatccag cggaatacgc tgactaaaaa tcgctaccgc aaaggtccag
    50701 ccgctcatca gcagcaccca cagcaggagc gagccttcat gcgcgcccca ggtggccgcc
    50761 acgcgatacc acaccggaag ctgggtattg gagttgctgg caacataggt gacggtgaag
    50821 tcgttgacca cgaaggcatt gaccagtacc agaaatgcgc cagccacaga cataaacagc
    50881 agccaggcaa acaagcggga agatgccatc atgcgcgcat ctccgcgcgc tacgccccat
    50941 agcggataca cggacagcag cagcgcaatt cctagcgcca gacacagcag cccgttacca
    51001 atttccggca tcatgatgct gggtccttat aaacactcgc cgggcgacgg tgattggctt
    51061 ccatcgcttt ctcaacttct ggcggcgtgt agttttcatc gtgtttcgcc agcacttctt
    51121 tcgcgaggat atgattgcct tcttccagtt cgccctgcac cacaacgccc tgcccttcac
    51181 ggaacagatc cggcaaaatg ccttcgtaag agacatctac tgaaccttca gcgtcgtaaa
    51241 tggtgaaagt tacttttagc gaattgggat cgcgctgcac actacccggc atcaccatcc
    51301 cgccaacgcg cagacgctga ccgacttccg gcatttgctg agtttcacgc ttgccgtaga
    51361 gaatttcccc cggcgtataa aagagatcga tattcgagcg cagcgcatat agcaccagac
    51421 cgatagtcag cgccagcccc gccaacacgg cacaggcaat ccacaagcgg tttttacggc
    51481 gaatattcat gcagcctcct gctgttgcgc agcacgtaaa cgcgcctcac gcgcccgctg
    51541 ttgcgccacg ccacgcagaa ttgcgcgatg ctgcatcacc gagtgcacga ccaaaaccac
    51601 cagcggaatg acggtcatca ccaccgccag ccagacaaaa aaggcgtaac cgcccattgc
    51661 gaaaaattca ttccaggaag caaatgcagg ggtcatttac ggcctctttt cagtatcagt
    51721 tcactcaccc acggacggcg tttttccatc agcaaaatca aattacgcat ccgcatcagc
    51781 gtcagcgtgg cagacaggag caggaagcca aaaatcgacc agcgcagcgg cgaacgcatc
    51841 gccggatcga tactttgctg catccgcgtt gatccctggt gcagggtgtt ccaccactcc
    51901 acggagtaat gaataatcgg cagattcacc acgccaatca gcaccaggat acctgccgca
    51961 cggcccgcca gacggcggtc gtcgaaggcg tgccacaggg caatcacacc cacatacaaa
    52021 aacagcagca ccagttcaga agtcagacgt gcatcccata cccaccaggt gccccacatc
    52081 ggttttcccc atgcagagcc ggtaaccagg gcaataaagg taaacacggc accaatgggg
    52141 gccatcgccg ccaccgccag gttggccatt ttcatctgcc agacaaggcc aataaacgcc
    52201 gccaccgcca ttgatgcata aatgcccatc gaccagatcg ccgcaggcac atgcaggtag
    52261 ataatgcggt agctatttcc ctgctgataa tcagccggag caaagccgaa tccccagatc
    52321 cagccgacgg taagcacgac cacactggca attgccagcc acggtataaa ccagccacag
    52381 atttgataca gccgtggtgg gatcgccagt tgatgcagtg ttttccacat agtttcgata
    52441 ccagactcga acaaaaatca gtaatccagc gttattgaat gctgattcgt aacgctgccg
    52501 ccgtcgcaaa aggacttaat gtcgcggtgc ctgccagcaa cgcgcctaaa attgccagat
    52561 acccatcaac gggcaaatgc atagaagccg cgtccatcgc ggcggtggca aagatgagta
    52621 atgggatagt cagcggtaac accagtatgc tgagcagcac accaccgcgc ttaagtccca
    52681 ctgtcagcgc cacgcccggt gcaccgagaa agccaagcgt aggcgttccc agcagcagcg
    52741 tcagcgccat cacttgccag ccataaacat ccattcccag tagcattgct accaggggcg
    52801 aaaggatgag taacggcaga ccggttacca tccagtgcgc catcaccttc gccagcacaa
    52861 cggcgggcaa gggtaacggc aacaacatca attgttcaag actgccgtcc tgcaaatcgt
    52921 cacggaacag tcgctcgagt gccagcaagg atgaaagcag cgcggcaacc cagataatgc
    52981 ccggtgcaat acgcgccagc agttgcggct ccggaccgat actgagcgga aaaagggtaa
    53041 tgacaatcag gaagaaccac agcgggttgg cgatttcggc gctgtggcga aacgccacgc
    53101 gcagctcaag acggaaaatg cgccagaaca tcatgcggcc cccgtttgcg tcagtgaaat
    53161 gcggcgaatt ttactttcag caacgttgag cggctggtgg gtagtcagaa tcacagtccc
    53221 cccctgctcc gtatgctgcg ccatacgctg ggtcagacga tcgacgccgt taacgtcaat
    53281 cgcggtaaaa ggctcgtcga ggatccataa cgtggcacgg gtcagccaca gacgcgctaa
    53341 agcgacgcgg cgttgttgcc cggcagaaag ttgatttacc ggaatatctt cgaatccggc
    53401 aagcccagcc tgcgccaggg cttccagaca ttgtgcggta tcgccatcgc gatgataaaa
    53461 gtgcagattt tctaacgccg tcagccgggt tttgatcccc ggctgatggc ctatccataa
    53521 caggttttga tggtagctgt cgcgtacctg atgcaagggc tgcccttgcc agagaacgtc
    53581 acccgcatca gggcgagaca accccgtcag caaacggaga agcgttgtct tccccgcgcc
    53641 gttgctaccg gtgatttgta cccactctcc tgcgttcagc gtaaatgaca agccactaaa
    53701 taaggttcgt tcatcccgct cgcaaagtaa ctctctggct tcaagcatac ccacgcaata
    53761 accctgttaa aaacctggct cgacttcgcg catatcgggc agcttgtgcg ctatcccttt
    53821 atggcaatca atacaggttt gcccatcttt caccgcctgg tcatgcatct tcgcggcaac
    53881 cgatttctgg gcggttgtat ccatatactc gaagttgtga cagttacggc actcctgcga
    53941 gttattgtcc ttcatgcgcc gccactcatt ctgtgccatc gtcaggcgat gagcttcaaa
    54001 tttctgcggc gtgtcaataa cgccaaaaat tttaccatac agctctttac ttgctttgag
    54061 cttgcgtatc atcttcggca caaactcgtg cggaacgtga caatccggac aggtcgcacg
    54121 aacgccgcta cggttgttgt agtgcacgga atccatgtat tcctgataca ccgtgttacg
    54181 catttcgtgg cagctaatgc agaactcttc ggtattggct ttttccatcc cggtgttaaa
    54241 gccgccccag aagacgatgc cgccgacaaa accgatcaac agtagcgtcc ccagcgccaa
    54301 acggctgggg gtacgccacc atttccacag gcgcttaatc agaccaggct tacggtcaga
    54361 atttcccata ataacctctt atttcccgta accttttgat ggggtaaagg tattccccac
    54421 gattggcgcg gtatcggcct gcggtacgtg acattgcaga cagaaataac gacgcggagc
    54481 cacttccgcg cccactttgc cgtcgctgtc cataaagtga gtaggactga tgcgcggcgc
    54541 gccagtggtg cgatagcttt cgacaccgtg gcactgcaag cagcggttgg tattggtcgt
    54601 tacctgataa ccttcaacgc tatgcgggat cattggcggc tgattcacat agttcagcgg
    54661 catccgttcc tgctcttttg gcatccgaat ggccccttcc tgcgtcccgg atacttccgg
    54721 cgactggcta aaatcgacgc cattagccgc ccaaaccgcg ccgctcacca ccagggccag
    54781 catcgccgtc cattgacaca gcgctttctt caggtcatgg cttttcatga tttcgctccc
    54841 gaactccatc gtgtagttat tgtaaataca tcctcagaac agacatccac gcagcgaccg
    54901 cacgtcatgc aatcgcggct ggtgacctgc accgggcttt gttcatccag caccggggca
    54961 cgtagcacat gcggttccgg gcaaacatga aaacaatcca tacagcggtt acatttctgc
    55021 cgatcgctgg ccgcaacggt aatcacacct ttgctaccca gtacgccata cagcgcgcca
    55081 accggacaaa tgtgaccgca ccagccgtgt tcaacgacca gtagatcaaa taaaaacagc
    55141 gcgagaataa gcagcgcgcc gctaccgaag cccattacca ggctgcgccc catcagggaa
    55201 acggggttga tccattccca gatgagcgtg ccggttaagg ccgatcccag cagaatgacc
    55261 accaacagca cgtagcgaat gtggcgaggg atcgtcgcag actgattgag gtcaaagcgc
    55321 ctgcgtaacc agttcgctaa gtcggtaatc gggttcagcg ggcagaccca gctgcaaaat
    55381 aatcgctttc ctgccagggc gtagagcacg gtgataatta ccgcaccagt cagtgctacc
    55441 gtggcaggca gatgaccgct ggcaaggctt tgcagcgtca tcagcggatc ggtcagcgga
    55501 acggtgtcga ataacaggct actgctgtag ttgccgtgca agatccacac accaaaccac
    55561 ggaccgctca aaaacatccc cagcacgaag aactgacaaa ggcgacgtaa cactaaccaa
    55621 cggtgactgc gccaccagcc ttttttctcc agcgcctcgc gcccggcgtc acgtttacga
    55681 tttgccattg ttcccctcca gccagccgaa gcggtaatgg tgacctaact cccctttcgc
    55741 tagtgacagc ggtaacacct tgattgccgg ttgttccagc acgcacactt tttcgcactt
    55801 accgcaaccg gtacaggcgt cgctgtgaac cgtcggcaga aagcgggcgt gcttaccggt
    55861 acgcgtgttg cgctccagct ccagggtgat ggcctcatca attttcgggc attcgcgata
    55921 acaaacatcg cagcgcagcc cctgaaagtt gagacagttt tcctggtcca ccagcaccgc
    55981 cagccccatc cgcgcgtcgt cgatcgattc gatctcacga tccagcgcac cgctcgggca
    56041 cactttggcg cacggaatgt cctcacacat ttcgcaagga atatcccgcg caacaaaata
    56101 tggcgtgccc gccgacaggc cagaagccag cgtcgccagt tttaaggtgt cgtaagggca
    56161 agcctgaaca cactgaccgc aacgcacaca ggcactggca aaggcgttct cgtttatggc
    56221 tccgggcggg cgcaaccgca caccagatgc gcgtgcggtt tgctgttgta accccagcgc
    56281 cacacccacg gcagccagcc cgcctgctgt gcgaacgaca tcgcgcagaa agcggcggcg
    56341 accattttga ggtttcgctg accgggacat aatggcttac accttctcca gtttgaccgc
    56401 gcacttcttg aagtccgtct ctttcgagag cggatcggtc gcgtccagcg tcagtttgtt
    56461 aaccagctgt gcggcgtcga agaacggcat gtacaccagg ccctgcggtg gacggttacg
    56521 gccgcgcgtt tcgacaatcg agatcacttc gccacggcga gaaaccactt tcactttgtc
    56581 gccacgacgc agatcgcgcg ctttcgcatc cagcgggtga ataaacagga ccgcttcagg
    56641 gaaggcgcgg tgcagttccg gtacacggcg agtcatactg ccggtatgcc agtgctccag
    56701 gacgcgtccg gtagagagcc acaggtcgta ctcttcatcc ggtgcttccg ccgcaggttc
    56761 gaacggcagc gcgaagatca ccgctttgcc atccggttta ccgtagaact tgtagccttc
    56821 gcccgctttc acgtacgggt cgttaccttc gctgtaacgc cactgcgttt ctttaccgtt
    56881 caccaccggc cagcgcagac cgcgtgcttt gtggtagtca tcgaacggcg cgagatcgtg
    56941 accgtgaccg cgaccaaacc atgcgtactc ttcgaacagc cctttttgca gatagaagcc
    57001 cagctcgcgg gattcatcgt tcagctgatc ttccgccagt tcggatagcg ggaatttgct
    57061 cacttccgga gtggcataca gaacctcata cagcgttttg ccacgcagtt caggtttctt
    57121 cgccagcaac tcttccggcc acacttcttc agttttgaag cggcgggaga actgaactaa
    57181 ctgccagaga tccgatttcg cttcacccgg tgcctgtacc tgctgacgcc agaactgagt
    57241 acggcgttcg gcattaccgt aagcgccctc tttctctacc cacattgcgg tcggcaggat
    57301 caagtccgct gccagcgcac tgaccgtcgg atacggatcg gaaacgatga taaagttgcg
    57361 cggatcgcgc cagcccggca tacgctcttc attaatgttc ggtccggcct gcatgttatt
    57421 ggtacacatg gtccagtaaa cattcagttt gccgtctttc agcgcacggt cttgtgccac
    57481 cgcgtgcaga ccgattttcg ccggaatggt gccactcggg atattccact tcttctcgca
    57541 aatatcacga tgtttctcgt tagtcaccac catgtccgca ggcagacggt gagcaaaggt
    57601 gcccacttca cgcgcagtac cacacgcgga aggctgcccg gtcagggaga acggaccgca
    57661 acccggctgg gaaattttgc cggtcagcag gtgcaggttg tagaccaggt tgttggccca
    57721 cacaccacgg gtatgctggt tgaagcccat cgtccagtag gagatgactt tcttgttcgg
    57781 atcggcatac agctgcgcca gttgttctaa ctggtctttc ggcacgccgg tcatttcggc
    57841 ggttttttcc agcgtatatt cagcaacgaa ggctttgtaa tcttcaaagc tcatcggttc
    57901 ggaggcgtca gaacccggat tcttcgctgc tttttccagc ggatgggtcg gacgtaaacc
    57961 gtagccgatg tccgtcgccc ctttgcgcag gttaacgtgt ttgctgaaga agtcctgatt
    58021 tatcgcattg ttttgaatga tatagttggc gatatagttc aggatcacca ggtcagattg
    58081 cggcgtaaag atgatgccgt tatccgccag ctcgaagcta cgatgctggt aggtagaaag
    58141 caccgccacg gtaacgttct ggttagagag acgacggtta gtgatgcgcg accagaggat
    58201 cgggtgcatc tccgccatgt ttgcgcccca cagcacaaac gcgtcagcct gctcgatgtc
    58261 gtcgtagcag cccatcggct catccatacc aaaggtacgc ataaagccaa ctactgccga
    58321 cgccatacag tgacgcgcgt tcgggtcgat gttgttcgaa cggaagcccg ctttgaacaa
    58381 cttggacgcg gcataacctt cccagatagt ccactgacca gaaccgaaca taccgatcga
    58441 ttccggccct ttttctttca gggcagtctt aaatttctct tccatcacat cgaaggcctg
    58501 atcccaggtg attggggtaa attcgccttc tttgtcatat ttaccgtttt tcatacgcag
    58561 cagcggctgc gtcaaacggt ctttaccgta catgattttg ggcaggaaat agcccttaat
    58621 gcagttcagg ccacggttaa ccggtgcgtc cgggtcgccc tgacaggcca ccacacgccc
    58681 ctgctgcgtt ccgaccagaa cgccgcaacc cgtaccgcag aaacggcacg gcgctttatc
    58741 ccatttaatg gcttcctgct gaccaacaac ggcgcgggca acgcccggca cgctgagacc
    58801 ggcagccgcc gcagcggccg caacggcgtt agctttcata aagctacgac gactgagttt
    58861 catggtgttt cctcaccttg ctcttcctgc tggtgataaa ccagcgacac cgccagcacg
    58921 ccctctacgt tgcgtactga ctcaatggtt tggatcagcg tttcgctgtc ttctgcttcc
    58981 accaccacaa tcaactgacc gctcggcgcg tcgctgacag caacttcaca gccggtaaag
    59041 gcgttcagtt gggtgctgat gtctgaaatt cgttcgcttt tggcctgcac gaccaggctg
    59101 caaacttgcc agttagtgtg catggagata ctccgcagtt atggctgata ccggacagct
    59161 ggctgcacat gctccacatc cgttgcaaag ttggctatta agttgcggct ggtagatccc
    59221 ggagagcgtc gggcgaaaga taattgccat tggctcacag ctatcctgac agcggcggca
    59281 ttcaactgac tgatacgcca ggcacgcgtc cccaatggtg aactgcaaat cccaggccct
    59341 ggtgtggcgc ggagaaaata gcgattcggg gcaagcctgc gcgcaggcgt aacagaagct
    59401 gcactcgtta tttttgaaat taacgctcgg atagccaccc gcgccgcgtt gcagaatgtt
    59461 gttttcacaa gcattaatac agacatcaca gcgggtgcaa tgggtcagaa aatgagattc
    59521 atcaccggac cagggcggac ggataccgtt actggctttg cgccagcgac cagtgagtat
    59581 gccccgacgg gatgcatcaa tcttcacatt gaccttccat cattaacgcg ctctgaaaat
    59641 tgagagcgac caaataaacc gcataattaa taagccattt ttatagccgc taagatatta
    59701 aaggatgtgt caaagatgca taccccgatc ggggtaaaac ctgtagcagg atcaaaagag
    59761 tggcgggaag cgtggcaaaa acgggctttt gctcacattt caaatggtta taaacatatt
    59821 tatatagcga ttaattcgcc agagatattt ctgctggttt gttttctcat cagaatttaa
    59881 cactaaaaga gcaggtaaaa ttatctgaat gttctttaag ttattcataa agcaaattaa
    59941 taaatctgat gaatatgtta accttcagcg acatcatcgg tgaaaaccta taaatgaaga
    60001 aggaaagcaa aaaaatgaag accattctac ctgcagtatt gtttgccgct ttcgccacca
    60061 cttccgcctg ggcggcagaa agcgtccagc cactggaaaa aatcgcacct tatccacaag
    60121 ctgaaaaagg catgaagcgt caggtgattc agttaactcc gcaagaagat gaatctaccc
    60181 tgaaagtaga actgttaatc ggtcagacgc tggaagtcga ctgcaatttg catcgtctcg
    60241 gcgggaagct ggaaagcaaa acgctggaag gctggggcta tgattattat gtctttgata
    60301 aagttagctc gccggtttcc acgatgatgg cttgcccgga tggcaagaaa gagaagaaat
    60361 ttgtcaccgc gtatctgggc gatgctggaa tgctgcgtta caacagcaag ctgccgatcg
    60421 tggtgtatac gccagacaat gtggatgtga agtaccgtgt ctggaaggcg gaagagaaaa
    60481 ttgacaacgc ggttgtccgc taaactgctg tgaagtgctg caacctgtag gccgaataag
    60541 gcggttacgc cgcatccgac atctaacgcc cgagccggtt gcctgatgcg acgctgacgc
    60601 gtcttattag catacgccac atccggcaaa ccatgccgga tgtggcacat cattacaacg
    60661 caatatccgc cacttctttt tgaacgggtt gcggtttcaa ctgcggtttc ggcgtactat
    60721 ctgctgcctg cggcttgtcg tatttcagcc ccagcacttc gctggtgtac tgcaactcgc
    60781 gttctgttgc cgctacatca ccgttcagct tgcgtccata agacggaacg gtcgccttca
    60841 acgttgcctg ccattgcggg ctggaaacgc gatcgccaaa tactttttcc agcagattca
    60901 acataatcgg cgcggcggtt gacgcacctg gcgatgcccc cagcagcgcg gcaatggtcc
    60961 cttgctggtc gctgaccact tcggtaccca gacgcagtac gccacctttc tcggcatcac
    61021 gcttgataat ctgtacgcgc tgccccgctt gccacaaacg ccagtcctct tttttcgcct
    61081 gcggatagta ctctttcaac gcttcaaaac gatcctcttc actcaacatc acctgactca
    61141 ccagatattt caccagatcg aaattatcca gcccgacgtg catcatcggc atcacgttag
    61201 aggtggtggt ggaactcatc agatcccaca atgaaccgtt tttgaggaat ttggttgaga
    61261 aggtggcaaa tggcccaaac agcacgacgc gtttaccgtc cagaacacgg gtatcgatat
    61321 gcggaaccga catcggtggt gcgccaacgg atgctttacc gtaaaccttc gccagatggt
    61381 gattaaccac gtccgggttt tccgaaacaa ggaactgtcc gcccaccggg aaaccggcgt
    61441 agtctttcgc ttccggaatc cccgattcct gtaacagctt cagcgccgca ccgcccgcgc
    61501 cgataaagac aaatttggcg cggatgttct gtgcagtgcc atttttcaga tcggcaacgg
    61561 taacggtcca ggtgttatcg tcattacgct ttagggcgcg gacttcgctg ctgagttgca
    61621 gcgaaaaatt agatttcttc tgcaaggaag caattaactg gcgggtgatc tcgccatagt
    61681 tcacatcagt accaatttcc gtacgcgtgg ctgccacttt ctgttgcgga tcgcgccctt
    61741 ccatcactaa cggtgcccac tctttgatct gcgcgtgatc ttcagagtaa cgcataccgc
    61801 gaaacagcga gctttgttgc aacgcggcgt aacgggcgcg caggaaatta acattatcct
    61861 cgccccagac aaagctcata tgcggaacgg tattgataaa tgaacgcggt gtacgcagca
    61921 cgccgcgctc gacctggtgc gcccagaact ggcgcgaaat ctgaaatgct tcgttaatgg
    61981 cgactgcttt ttcaatactg atgctgccat cggcgttttg cggggtgtag ttcagttcca
    62041 tcagtgcaga atgcccggtt ccggcgttgt tccagccgtt cgaactctcc tgcgcgacac
    62101 cctccaggcg ctccaccatg gtcatcgacc attcaggctc cagctcgcgt aaataggtcc
    62161 ccaacgtggc gctcataatg ccgccgccaa tcaacagtac atcagtttcc tgctcctcgg
    62221 acgctttcgc ttttgccgcc atcgaaacgg cattaagccc cacggccatc gagaagagca
    62281 tggcagtcac ttttttcatc ttgttaatgc cttactttta gtcgctttat tgcaggtgag
    62341 atttgcgcgg caccaacggt aacacttaag taacaacatt taaataatgt ttataaatta
    62401 tattctaatt ttagaaacat tataatttcg ttgattaatt atagggttat tagaaacgaa
    62461 gggattttta gcatattgac ctggcggcag cagtccgcca ggtcagcgtc gaaatattat
    62521 gccgtccggg caacggcatc acgcgaagca gcatcgcgct cttcgcctgt cagctcgcta
    62581 agttgcccgt tgcgcatttc cagcaggcgg tcagcgtgga taaagtaatg atcatcatga
    62641 ctgatggcga atatggtttt acccatctcc tgcatcagcg gcagcagcac ctgataaaac
    62701 tcacggcgga agtgtgggtc ctgatccgcc gcccattcat ccagcaggat aatatcgcgt
    62761 tcttctgcca gcgccagcaa cagcgcgacg cgttttttct gcccttttga taacttcagg
    62821 ttaacaatac gcccgttgct taactcaagc ttatgagcca ttttcagttg cgccagccac
    62881 ttctcaatca gttgcgggtt agcgggttga ccctccggcc ccagcagttg atcgaacagc
    62941 cagacatcgg taaacactgc cgaaaacagt ttgcgataat cttccggttg ttcgccgctg
    63001 acaggtttgc catccagcaa gatttcgccg ctttgtggct gatacaagcc cgtcagcaac
    63061 atcgccagcg tcgattttcc actaccgttg ccaccaatca gaaacagcag ttcgccacgt
    63121 ttgatggtga ggttaatcgg cccaacggaa aacgcgttat cctgataagc aaacgtaaca
    63181 ttgcgcagct ccagcgtttg ccagttggga aacgtctgcg gacgcggaaa ctctgctttg
    63241 aaaggcgcga gcgcgaattt gttcagcttg ttaaacgcca cctgcgccgt cagcagcgtc
    63301 ggtaatgcgc caaccgccga aagcagcggc gtacgcagga acaacagcgt caacgaatag
    63361 gtcgcggcaa cgttggtatc agcccaaccg agactgttcg ccatccagaa caccaggccg
    63421 atagcgccca gcatcatgat gtttgaccag ttcacggcac taagatggaa ggtgtctgcg
    63481 cggataatat gatggcgata ctcttgcgca tcaggaatgt agaggttgtt aaacacatac
    63541 tcggcgcgtt cccggttcag agtcagctct ttgcgccctt cgagtacggt ttgaaaatcc
    63601 gtgtacagct tatcttcggt ttcacgcagg gtcgccatat gtttgtacac ccgcgccacc
    63661 agcacaaaac cgccccagat ggtgactgcc atccagatag ccgttaccag caacattttg
    63721 cccgacagca tccacaaata cgccgcggaa ccgatagtga gaatgatccc ctgcaccagt
    63781 tccggcagac gcacaaaagc aatggtgata ttgcgcacat cgctggttaa ccccgccagc
    63841 aacgaagcgc taccaagttg ttcaatgcgc tcgacgtggg tatccagaat ccgcttgata
    63901 aattcactac gcagtcggta aacgaagtga tgccccaaag tggtgagcgc cagttgcgat
    63961 ccgagagtga ctgccatcaa cagcaacaat aatcccagaa actccggcaa caccagcaga
    64021 ctggtatccg ccgtttcgat aaggcgctga ttgataaaag caattaagcc aatgcctaat
    64081 gccgcactgg cgaggcttag cgccatcaca ctgataaatg gccagcgata ctgccgccag
    64141 acaagtacaa gaagttccat gcagaaaacc cggacaatga attacagccc gcagtttaaa
    64201 catcttcgcg cgcacagcaa taataattct tatttttatt cttttttacc tgcctgacgg
    64261 aatgtcaggt tgtagcggca gtcgccggcg agtggatgaa accccgcttt caacggttga
    64321 atgccatgat aaaacagccg cgattcaccg ccccatacca ccacatcgcc atgttccagc
    64381 aacaatcttt ttagcggatc atttcgtttc aggccgccaa attgaaaaat cgcgggtaag
    64441 cccagagaaa cagaaacaat tggcgcgcgc agatccggtt cgtctttatc ctgatgcagc
    64501 gacagtttcg cgccaggaac gtagcggttg atcaaacagg catctggctg gaaatctgga
    64561 tagccccccg ccgtagccgc acgttgacat aaatgatgaa aactctgcgg catggcgggc
    64621 cacggtttat ttgtttgcgg atcaatgggc gaatagagat aaccttgccg atgggtcgtc
    64681 cagcccagat gcccacagtt ggtcatcgcc accgacatgg tatatccgcc tggagtgacc
    64741 atctggcgaa acggcgactg gctggcaacg tcattaatat cgcggattag ttgctccgca
    64801 gcgttaaaag cgaaacgccg taaaattacc gcaccagccg ccagtggctc ttgccacggt
    64861 tcagcatcgg caaacagatc caacattacc tctcctcatt ctcagcttcg cggcgcagca
    64921 gttgcgcttt gcgcgacacg ccccagcggt aaccggaaag tgtgccatca ccacggacca
    64981 cccgatgaca aggtataact atagctagct tgttggcggc acaggcgctg gcaacagccc
    65041 gtaccgcttt cggtttgccg atggcattag ccagttgctg ataactgacg gtttcaccgc
    65101 aaggtatcgt gcgcagtgcc tgccagactt gttgctgaaa agcagtgccg cgaatgtcca
    65161 gcggtaacgt cagcggcgta tcgcgttgat tgaggctggc gatcacttca cgcacatgtt
    65221 gctgaaacgt cagatcggca ggcgcgctgt cggcagcggg aaacatctgc tgtaactcgc
    65281 tgattagtgt cgcgtcatca tcgcccagta atatcgcgca aatcccccgc tcactttctg
    65341 ccaccaggca acggcccagc tcacaatcag ccagcgcgta acgcaccgcc agattttcgc
    65401 caccgtgacg gaattgttta gccgtcatgc ccagcgtttc gtcagctttg cggtagtaac
    65461 tgctgctgtc ggggaatccg gcgttaagaa tagacgtcgt cacgctctcc cctttcgcca
    65521 gcgattcgcg caaac
//
LOCUS       DAMZFT010000011        62403 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.011, whole genome shotgun sequence.
ACCESSION   DAMZFT010000011 DAMZFT010000000
VERSION     DAMZFT010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 62403)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 62403)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..62403
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.011"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(<1..355)
                     /locus_tag="PN977_000819"
     CDS             complement(<1..355)
                     /locus_tag="PN977_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HDI6040882.1"
                     /translation="MKEKTIIIVGGGQAAAMAAASLRQQGFTGELHLFSDEQHLPYER
                     PPLSKSMLLEDSPQLQSVLPAHWWQENNVHLHSGVTIKTLGRDTRELVLANGESWHWD
                     QLFIATGAAARPLPLL"
     gene            complement(365..1177)
                     /gene="hcaB"
                     /locus_tag="PN977_000820"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
     CDS             complement(365..1177)
                     /gene="hcaB"
                     /locus_tag="PN977_000820"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
                     /EC_number="1.3.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate-dihydrodiol/cinnamic
                     acid-dihydrodiol dehydrogenase"
                     /protein_id="HDI6040883.1"
                     /translation="MSDLHNESIFITGGGSGLGLALVERFIEEGAQVATLELSAAKVA
                     SLRQRFGEHILAVEGNVTCYADYQRALDQILTRSGKLDCFIGNAGIWDHNASLVNTPA
                     ETLETGFHELFNVNVLGYLLGAKACAPALIASEGSMIFTLSNAAWYPGGGGPLYTASK
                     HAATGLIRQLAYELAPKVRVNGVGPCGMASDLRGPQALGQSETSIMQSLTPEKIAAIL
                     PLQFFPQPADFTGPYVMLASRRNNRALSGVMINADAGLAIRGIRHVAAGLDL"
     gene            complement(1174..1494)
                     /locus_tag="PN977_000821"
     CDS             complement(1174..1494)
                     /locus_tag="PN977_000821"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-phenylpropionate/cinnamic acid
                     dioxygenase ferredoxin subunit"
                     /protein_id="HDI6040884.1"
                     /translation="MNRIYACPVADVPEGEALRIDTSPVIALFNVGGEFYAINDRCSH
                     GNASMSEGYLEDDATVECPLHAASFCLKTGKALCLPATDPLTTYPVHVEGGDIFIDLP
                     EAQP"
     gene            complement(1494..2012)
                     /locus_tag="PN977_000822"
     CDS             complement(1494..2012)
                     /locus_tag="PN977_000822"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate/cinnamic acid dioxygenase
                     subunit beta"
                     /protein_id="HDI6040885.1"
                     /translation="MSAQVSLELHHRISQFLFHEASLLDDWKFRDWLAQLDEEIRYTM
                     RTTVNAQTRDRRKGVQPPTTWIFNDTKDQLERRIARLETGMAWAEEPPSRTRHLISNC
                     QVSETDIPNVFAVRVNYLLYRAQKERDETFYVGTRFDKVRRLEDDNWRLLERDIVLDQ
                     AVITSHNLSVLF"
     gene            complement(2009..3370)
                     /locus_tag="PN977_000823"
     CDS             complement(2009..3370)
                     /locus_tag="PN977_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HDI6040886.1"
                     /translation="MTTPSDLNIYQLIDTQNGRVTPRIYTDPDIYQLELERIFGRCWL
                     FLAHESQIPKPGDFFNTYMGEDAVVVVRQKDGSIKAFLNQCRHRAMRVSYADCGNTRA
                     FTCPYHGWSYGINGELIDVPLEPRAYPQGLCKSHWGLNEVPCVESYKGLIFGNWDTSA
                     PGLRDYLGDIAWYLDGMLDRREGGTEIVGGVQKWVINCNWKFPAEQFASDQYHALFSH
                     ASAVQVLGAKDDGSDKRLGDGQTARPVWETAKDALQFGQDGHGSGFFFTEKPDANVWV
                     DGAVSSYYRETYAEAEQRLGEVRALRLAGHNNIFPTLSWLNGTATLRVWHPRGPDQVE
                     VWAFCITDKAASDEVKAAFENSATRAFGPAGFLEQDDSENWCEIQKLLKGHRARNSKL
                     CLEMGLGQEKHRDDGIPGITNYIFSETAARGMYQRWADLLSSESWQEVLDKTAAYQQE
                     VMK"
     gene            3506..4396
                     /gene="hcaR"
                     /locus_tag="PN977_000824"
     CDS             3506..4396
                     /gene="hcaR"
                     /locus_tag="PN977_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HcaR"
                     /protein_id="HDI6040887.1"
                     /translation="MELRHLRYFVAVAQALNFTRAAEKLHTSQPSLSSQIRDLENCVG
                     VPLLVRDKRKVALTAAGECFLQDALAILEQAENAKLRARKIVQEDRQLTIGFVPSAEV
                     NLLPKVLPMFRLRQPDTLIELVSLITTEQEEKIRRGELDVGLMRHPVYSPEIDYLELF
                     DEPLVVVLPVDHPLAHEKEITAAQLDGVNFVSTDPAYSGSLAPIVKAWFAQENSQPNI
                     VQVATNILVTMNLVGMGLGVTLIPGYMNNFNTGQVVFRPIAGNVPAIALLMAWKKGEM
                     KPALRDFIAIVQERLASVTA"
     gene            4556..5695
                     /locus_tag="PN977_000825"
     CDS             4556..5695
                     /locus_tag="PN977_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate MFS transporter"
                     /protein_id="HDI6040888.1"
                     /translation="MVLQSTRWLALGYFTYFFSYGIFLPFWSVWLKGIGLTPETIGLL
                     LGAGLVARFLGSLLIAPRVSDPSRLISALRVLALLTLLFAVAFWAGAHVAWLMLVMIG
                     FNLFFSPLVPLTDALANTWQKQFPLDYGKVRLWGSVAFVIGSALTGKLVSMFDYRVIL
                     ALLTLGVASMLLGFLIRPTIQPQGASRQQESTGWSAWLALVRQNWRFLACVCLLQGAH
                     AAYYGFSAIYWQAAGYSASAVGYLWSLGVVAEVIIFALSNKLFRRCSARDMLLISAVC
                     GVLRWGIMGATTELLWLIMVQILHCGTFTVCHLAAMRYIAARQGSEVIRLQAVYSAVA
                     MGGSIAIMTVFAGFLYQYLGHGVFWVMALVALPAMFLRPKVVPSC"
     gene            complement(5687..6967)
                     /gene="csiE"
                     /locus_tag="PN977_000826"
     CDS             complement(5687..6967)
                     /gene="csiE"
                     /locus_tag="PN977_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary phase inducible protein CsiE"
                     /protein_id="HDI6040889.1"
                     /translation="MMPTLAPPSVLSAPQRRCQILLTLFQPGLTATTATFSELNGVDD
                     DIASLDISETGREILRYHQLTLTTGYDGSYRVEGTVLNQRLCLFHWLRRGFRLCPSFI
                     TSHFTPALKSELKRRGIARNFYDDTNLQALVNLCSRRLQKRFETRDIHFLCLYLQYCL
                     LQHHAGITPQFNPLQRRWAESCLEFQVAQEIGRHWQRRALQPVPPDEPLFMALLFSML
                     RVPDPLRDAHQRDRQLRQSIKRLVNHFRELGNVRFYDEQGLCDQLYTHLAQALNRSLF
                     AIGIDNTLPEEFARLYPRLVRTTRAALAGFESEYGVHLSDEESGLVAVIFGAWLMQEN
                     DLHEKQIILLTGNDSEREAQIEQQLRELTLLPLNIKHMSVKAFLQTGAPRGAALIIAP
                     YTMPLPLFSPPLIYTDLTLTTHQQEQIRKMLESA"
     gene            complement(7158..8012)
                     /locus_tag="PN977_000827"
     CDS             complement(7158..8012)
                     /locus_tag="PN977_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417029.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDI6040890.1"
                     /translation="MALPVNKRVLKILFILFVVAFCVYLVPRVAINFFYYPDDKIYGP
                     DPWSAESVEFTAKDGTRLQGWFIPSSTGPADNAIATIIHAHGNAGNMSAHWPLVSWLP
                     ERNFNVFMFDYRGFGKSKGKPSQAGLLDDTQSAINVARHRSDVNPQRLVLFGQSIGGA
                     NILDVIGQGDREGIRAVILDSTFASYATIANQMIPGSGYLLDESYSGENYIASVSPIP
                     LLLIHGKADHVIPWQHSEKLYSLAKEPKRLILIPDGEHIDAFSDRHGDVYREQMVDFI
                     LSALNPQN"
     gene            complement(8157..8960)
                     /gene="suhB"
                     /locus_tag="PN977_000828"
     CDS             complement(8157..8960)
                     /gene="suhB"
                     /locus_tag="PN977_000828"
                     /EC_number="3.1.3.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000553460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-1-monophosphatase"
                     /protein_id="HDI6040891.1"
                     /translation="MHPMLNIAVRAARKAGNLIAKNYETPDAVEASQKGSNDFVTNVD
                     KAAEAVIIDTIRKSYPQHTIITEESGELEGTDQDVQWVIDPLDGTTNFIKRLPHFAVS
                     IAVRIKGRTEVAVVYDPMRNELFTATRGQGAQLNGYRLRGSTARDLDGTILATGFPFK
                     AKQYATTYINIVGKLFNECADFRRTGSAALDLAYVAAGRVDGFFEIGLRPWDFAAGEL
                     LVREAGGIVSDFTGGHNYMLTGNIVAGNPRVVKAMLANMRDELSDALKR"
     gene            9079..9819
                     /gene="trmJ"
                     /locus_tag="PN977_000829"
     CDS             9079..9819
                     /gene="trmJ"
                     /locus_tag="PN977_000829"
                     /EC_number="2.1.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (cytosine(32)/uridine(32)-2'-O)-methyltransferase TrmJ"
                     /protein_id="HDI6040892.1"
                     /translation="MLQNIRIVLVETSHTGNMGSVARAMKTMGLTNLWLVNPLVKPDS
                     QAIALAAGASDVIGNAHIVDTLDEALAGCSLVVGTSARSRTLPWPMLDPRECGLKSVA
                     EAANTPVALVFGRERVGLTNEELQKCHYHVAIAANPEYSSLNLAMAVQVIAYEVRMAW
                     LATQENGEQVEHEETPYPLVDDLERFYGHLEQTLLATGFIRENHPGQVMNKLRRLFTR
                     ARPESQELNILRGILASIEQQNKGNKAE"
     gene            10090..10578
                     /gene="iscR"
                     /locus_tag="PN977_000830"
     CDS             10090..10578
                     /gene="iscR"
                     /locus_tag="PN977_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002913993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly transcriptional regulator
                     IscR"
                     /protein_id="HDI6040893.1"
                     /translation="MRLTSKGRYAVTAMLDVALNSEAGPVPLADISERQGISLSYLEQ
                     LFSRLRKNGLVSSVRGPGGGYLLGKDASSIAVGEVISAVDESVDATRCQGKGGCQGGD
                     KCLTHALWRDLSDRLTGFLNNITLGELVNNQEVLDVSGRQHTHDAPRTRTQDAIDVKL
                     RA"
     gene            10690..11904
                     /gene="iscS"
                     /locus_tag="PN977_000831"
     CDS             10690..11904
                     /gene="iscS"
                     /locus_tag="PN977_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017459011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HDI6040894.1"
                     /translation="MKLPIYLDYSATTPVDPRVAEKMMQFMTMDGTFGNPASRSHRFG
                     WQAEEAVDIARNQIADLVGADPREIVFTSGATESDNLAIKGAANFYQKKGKHIITSKT
                     EHKAVLDTCRQLEREGFEVTYLAPQRNGIIDLKELEAAMRDDTILVSIMHVNNEIGVV
                     QDIAAIGEMCRARGIIYHVDATQSVGKLPIDLSQLKVDLMSFSGHKIYGPKGIGALYV
                     RRKPRVRIEAQMHGGGHERGMRSGTLPVHQIVGMGEAYRIAKEEMATEMERLRGLRNR
                     LWNGIKDIEEVYLNGDLEHGAPNILNVSFNYVEGESLIMALKDLAVSSGSACTSASLE
                     PSYVLRALGLNDELAHSSIRFSLGRFTTEEEIDYTIELVRKSIGRLRDLSPLWEMYKQ
                     GVDLNSIEWAHH"
     gene            11932..12318
                     /gene="iscU"
                     /locus_tag="PN977_000832"
     CDS             11932..12318
                     /gene="iscU"
                     /locus_tag="PN977_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold IscU"
                     /protein_id="HDI6040895.1"
                     /translation="MAYSEKVIDHYENPRNVGSFDNNDENVGSGMVGAPACGDVMKLQ
                     IKVNDEGIIEDARFKTYGCGSAIASSSLVTEWVKGKSLDEAQAIKNTDIAEELELPPV
                     KIHCSILAEDAIKAAIADYKSKREAK"
     gene            12335..12658
                     /gene="iscA"
                     /locus_tag="PN977_000833"
     CDS             12335..12658
                     /gene="iscA"
                     /locus_tag="PN977_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein IscA"
                     /protein_id="HDI6040896.1"
                     /translation="MSITLSDSAAARVNTFLANRGKGFGLRLGVRTSGCSGMAYVLEF
                     VDEPTPEDIVFEDKGVKVVVDGKSLQFLDGTQLDFVKEGLNEGFKFTNPNVKDECGCG
                     ESFHV"
     gene            12754..13269
                     /gene="hscB"
                     /locus_tag="PN977_000834"
     CDS             12754..13269
                     /gene="hscB"
                     /locus_tag="PN977_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone HscB"
                     /protein_id="HDI6040897.1"
                     /translation="MDYFTLFGLPARYQLDTQALSLRFQDLQRQYHPDKFASGSQAEQ
                     LAAVQQSATINQAWQTLRHPLMRAEYLLSLHGFDLACEQHTVRDTAFLMEQLELREEL
                     DEIEQAKDEARLESFIKRVKKMFDTRHQLMVEQLDNETWDAAADTVRKLRFLDKLRSS
                     AEQLEEKLLDF"
     gene            13286..15136
                     /gene="hscA"
                     /locus_tag="PN977_000835"
     CDS             13286..15136
                     /gene="hscA"
                     /locus_tag="PN977_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly chaperone HscA"
                     /protein_id="HDI6040898.1"
                     /translation="MALLQISEPGLSAAPHQRRLAAGIDLGTTNSLVATVRSGQAETL
                     ADHEGRHLLPSVVHYQQQGHSVGYDARTNAALDTANTISSVKRLMGRSLADIQQRYPH
                     LPYQFQASENGLPMIETAAGLLNPVRVSADILKALAARATEALAGELDGVVITVPAYF
                     DDAQRQGTKDAARLAGLHVLRLLNEPTAAAIAYGLDSGQEGVIAVYDLGGGTFDISIL
                     RLSRGVFEVLATGGDSALGGDDFDHLLADYIREQAGIPDRSDNRVQRELLDAAIAAKI
                     ALSDADSVTVNVAGWQGEISREQFNELIAPLVKRTLLACRRALKDAGVEADEVLEVVM
                     VGGSTRVPLVRERVGEFFGRPPLTSIDPDKVVAIGAAIQADILVGNKPDSEMLLLDVI
                     PLSLGLETMGGLVEKVIPRNTTIPVARAQDFTTFKDGQTAMSIHVMQGERELVQDCRS
                     LARFALRGIPALPAGGAHIRVTFQVDADGLLSVTAMEKSTGVEASIQVKPSYGLTDSE
                     IASMIKDSMSYAEQDVKARMLAEQKVEAARVLESLHGALAADAALLSAAERQDIDDAA
                     AHLSEVAQGDDVDAIEQAIKNVDKQTQDFAARRMDQSVRRALKGHSVDEV"
     gene            15138..15473
                     /gene="fdx"
                     /locus_tag="PN977_000836"
     CDS             15138..15473
                     /gene="fdx"
                     /locus_tag="PN977_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015683702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISC system 2Fe-2S type ferredoxin"
                     /protein_id="HDI6040899.1"
                     /translation="MPKIVILPHQDLCPDGAVLEANSGETILDAALRNGIEIEHACEK
                     SCACTTCHCIVREGFDSLPESSEQEDDMLDKAWGLEPESRLSCQARVTDEDLVVEIPR
                     YTINHAREH"
     gene            15485..15685
                     /gene="iscX"
                     /locus_tag="PN977_000837"
     CDS             15485..15685
                     /gene="iscX"
                     /locus_tag="PN977_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004104502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein IscX"
                     /protein_id="HDI6040900.1"
                     /translation="MGLKWTDSREIGEALYDAYPDIDPKMVRFTDMHQWICALEDFDD
                     DPQASNEKILEAILLVWLDEAE"
     gene            15744..17027
                     /gene="pepB"
                     /locus_tag="PN977_000838"
     CDS             15744..17027
                     /gene="pepB"
                     /locus_tag="PN977_000838"
                     /EC_number="3.4.11.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417018.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase PepB"
                     /protein_id="HDI6040901.1"
                     /translation="MTEAMKITLSTQPADARWGEKATYSINNDGITLHLNGADDLGLI
                     QRAARKIDGLGIKHVQLSGEGWDADRCWAFWQGYKAPKGTRKVEWPDLDDVQRQELDN
                     RLMIIDWVRDTINAPAEELGPSQLAQRAVDLISNVAGDRVTYRITKGEDLRDQGYMGL
                     HTVGRGSERSPVLLALDYNPTGDKEAPVYACLVGKGITFDSGGYSIKQTAFMDSMKSD
                     MGGAATVTGALAFAITRGLNKRVKLFLCCADNLISGNAFKLGDIITYRNGKKVEVMNT
                     DAEGRLVLADGLIDASAQKPELIIDAATLTGAAKTALGNDYHALFSFDDALAGRLLAS
                     AAQENEPFWRLPLAEFHRSQLPSNFAELNNTGSAAYPAGASTAAGFLSHFVENYQQGW
                     LHIDCSATYRKAPVEQWSAGATGLGVRTIANLLTA"
     gene            17169..17945
                     /gene="sseB"
                     /locus_tag="PN977_000839"
     CDS             17169..17945
                     /gene="sseB"
                     /locus_tag="PN977_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311415.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enhanced serine sensitivity protein SseB"
                     /protein_id="HDI6040902.1"
                     /translation="MSETKNELEDLLEKAATEPAHRPAFFRTLLESTVWVPGTAAQGE
                     AVVEDSALDLQHWEKEDGTSVIPFFTSLEALQQAVEDEQAFVVMPVRTLFEMTLGETL
                     FLNAKLPTGKEFMPREISLLIGEEGNPLSSQEVLEGGESLILSEVAEPPAQMIDSLTT
                     LFKTIKPVKRAFICSIKENEEAQPNLLIGIEADGDIEEIIQAAGSVATDTIPGDEPID
                     ICQVKKGEKGISHFITEHIVPFYERRWGGFLRDFKQNRII"
     gene            complement(18442..19287)
                     /gene="sseA"
                     /locus_tag="PN977_000840"
     CDS             complement(18442..19287)
                     /gene="sseA"
                     /locus_tag="PN977_000840"
                     /EC_number="2.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-mercaptopyruvate sulfurtransferase"
                     /protein_id="HDI6040903.1"
                     /translation="MSTTWFVGADWLAEHIDDPEIQIIDARMASPGQEDRNVAQEYLN
                     GHIPGAVFFDIEALSDHTSPLPHMLPRPETFAVAMRELGVNQDKHLIIYDEGNLFSAP
                     RAWWMLRTFGVEKVSILGGGLAGWQRDDLLLEEGAVELPEGEFNATFNPEAVVKVTDV
                     LLASHENTAQIIDARPAARFNAEVDEPRPGLRRGHIPGALNVPWTELVREGELKTTDE
                     LDAIFFGRGVSYDKPIIVSCGSGVTAAVVLLALATLDVPNVKLYDGAWSEWGARADLP
                     VEPEK"
     gene            19494..24452
                     /gene="yfhM"
                     /locus_tag="PN977_000841"
     CDS             19494..24452
                     /gene="yfhM"
                     /locus_tag="PN977_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha2-macroglobulin"
                     /protein_id="HDI6040904.1"
                     /translation="MKKLRLAACMLMLALAGCDNNDNAPTAVKKDAPSEVTKAASSEN
                     ASSAKLSAPERQKLAQQSAGKALTLLDLSEVQLDGAATLVLTFSIPLDPDQDFSRVIH
                     VVDKKSGKVDGAWELSDNLKELRLRHLEPKRDLIVTIGKEVKALNNATFSKDYEKTIT
                     TRDIQPSVGFASRGSLLPGKVIEGLPVMALNVNNVDVNFFRVKPESLPAFISQWEYRN
                     SLANWQSDKLLQMADLVYTGRFDLNPARNTREKLLLPLGDIKPLQQAGVYLAVMNQAG
                     RYDYSNPATLFTLSDIGVSAHRYHNRLDIFTQSLENGAAQQGIEVSLLNEKGQTLTQA
                     TSDAQGHVQLENDKNAALLLARKNGQTTLLDLKLPALDLAEFNIAGAPGYSKQFFMFG
                     PRDLYRPGETVILNGLLRDADGKALPDQPIKLDVIKPDGQVLRSVVSQPENGLYHFTW
                     PLDSNAATGMWHIRANTGDNQYRMWDFHVEDFMPERMALNLTGEKTPLTPKDEVKFSV
                     VGYYLYGAPANGNTLQGQLFLRPLREAVSALPGFEFGDIAAENLSRTLDEVQLTLDDK
                     GRGEVSTESQWKETHSPLQVIFQGSLLESGGRPVTRRAEQAIWPADALPGIRPQFASK
                     SVYDYRTDSTVKQPIVDEGSNAAFDIVYSDAQGVKKAVSGLQVRLIRERRDYYWNWSE
                     DEGWQSQFDQKDLIENEQTLDLKADETGKVSFPVEWGAYRLEVKAPNEAVSSVRFWAG
                     YSWQDNSDGSGAVRPDRVTLKLDKASYRPGDTIKLHIAAPTAGKGYAMVESSEGPLWW
                     QEIDVPAQGLDLTIPVNKTWNRHDLYLSTLVVRPGDKSRSATPKRAVGVLHLPLGDEN
                     RRLDLALETPTKMRPNQPLTVKIKASTKNGEMPKQVNVLVSAVDSGVLNITDYVTPDP
                     WQAFFGQKRYGADIYDIYGQVIEGQGRLAALRFGGDGDELKRGGKPPVNHVNIVAQQA
                     LPVTLNEQGEGSVTLPIGDFNGELRVMAQAWTADDFGSNESKVVVAAPVIAELNMPRF
                     MASGDTSRLTLDITNLTDKPQKLNVALTASGLLELVSDSPAAVELAPGVRTTLFIPVR
                     ALPGYGDGEIQATISGLALPGETVADQHKQWKIGVRPAFPAQTVNYGTALQPGETWAI
                     PADGLQNFSPVTLEGQLLLSGKPPLNIASYIKELKAYPYGCLEQTASGLFPSLYTNAA
                     QLQALGIKGDSDEKRRASVDIGISRLLQMQRDNGGFALWDKNGDEEYWLTAYVMDFLV
                     RAGEQGYSVPTDAINRGNERLLRYLQDPGMMSIPYADNLKASKFAVQSYAALVLARQQ
                     KAPLGALREIWEHRADAASGLPLLQLGVALKTMGDATRGEEAIALALKTPRNDERKWL
                     GDYGSPLRDNALMLSLLEENKLLPDEQNTLLNTLSQQAFGERWLSTQESNALFLAART
                     VQDLPGKWQAQTSFSAEPLTGEKAQTSNLNSDQLATLQVTNSGDQPLWLRVDASGYPQ
                     SAPLPANNVLQIERHILGTDGKSKSLDSLRSGDLVLVWLQVKASNSVPDALVVDLLPA
                     GLELENQNLANGSASLEQSGGEVQNLLNQMQQASIKHIEFRDDRFVAAVAVDEYQPVT
                     LVYLARAVTPGTYQVPQPMVESMYVPQWRATGAAEDLLIVRP"
     gene            24453..26765
                     /gene="pbpC"
                     /locus_tag="PN977_000842"
     CDS             24453..26765
                     /gene="pbpC"
                     /locus_tag="PN977_000842"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417014.1"
                     /note="penicillin-binding protein 1C; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase PbpC"
                     /protein_id="HDI6040905.1"
                     /translation="MPRLLTKRGCWITLAAAPFLLFLAAWGADKLWPLPLHEVNPARV
                     VVAQDGTPLWRFADAEGIWRYPVTIEDVSPRYLEALINYEDRWFWKHPGVNPFSVARA
                     AWQDLTSGRVISGGSTLTMQVARLLDPHPKTFGGKIRQLWRALQLEWHLSKREILTLY
                     LNRAPFGGTLQGIGAASWAYLGKSPANLSYSEAAMLAVLPQAPSRLRPDRWPERAEAA
                     RNKVLERMAVQGVWSREQVKESREEPIWLAPRQMPQLAPLFSRMMLGKSKSDKIVTTL
                     DAGLQRRLEELAQNWKGRLPPRSSLAMIVVDHTDMRVRGWVGSVDLNDDSRFGHVDMV
                     NAIRSPGSVLKPFVYGLALDEGLIHPASLLQDVPRRTGDYRPGNFDSGFHGPISMSEA
                     LVRSLNLPAVQVLEAYGPKRFAAKLRNVGLPLYLPNGAAPNLSLILGGAGAKLEDMAA
                     AYTAFARHGKAGKLRLQPDDPLLERPLMSSGAAWIIRRIMADEAQPLPDGALPRVAPL
                     AWKTGTSYGYRDAWAIGVNARYVIGIWTGRPDGTPVVGQFGFASAVPLLNQVNNILLS
                     RSANLPEDPRPDSVSRGVICWPGGQSLPEGDSNCRRRLATWLLDGSQPPTLLLPEQEG
                     INGIRFPIWLDENGKRVAADCPQARQEMINVWPLPLEPWLPASERRAVRLPPASTICP
                     PYGHDAQLPLQLTGVRDGAIIKRLPGAAEATLPLQSSGGAGERWWFLNGEPLTERGRN
                     VTLHLTDKGDYQLLVMDEVGQIATVKFVMQ"
     gene            26914..27345
                     /gene="ndk"
                     /locus_tag="PN977_000843"
     CDS             26914..27345
                     /gene="ndk"
                     /locus_tag="PN977_000843"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HDI6040906.1"
                     /translation="MAIERTFSIIKPNAVAKNVIGSIFARFEAAGFKIVGTKMLHLTV
                     EQARGFYAEHDGKPFFDGLVEFMTSGPIVVSVLEGENAVQRHRDLLGATNPANALAGT
                     LRADYADSLTENGTHGSDSVESAAREIAYFFGEGEVCPRTR"
     gene            27495..28649
                     /gene="trmG/rlmN"
                     /locus_tag="PN977_000844"
     CDS             27495..28649
                     /gene="trmG/rlmN"
                     /locus_tag="PN977_000844"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA
                     large subunit methyltransferase RlmN"
                     /protein_id="HDI6040907.1"
                     /translation="MSEQLVTPENVTTKDGKINLLDLNRQQMREFFKDLGEKPFRADQ
                     VMKWMYHYCCDNFDEMTDINKVLRGKLKEVAEIRAPEVVEEQRSSDGTIKWAIAVGDQ
                     RVETVYIPEDDRATLCVSSQVGCALECKFCSTAQQGFNRNLRVSEIIGQVWRAAKIVG
                     AAKVTGQRPITNVVMMGMGEPLLNLNNVVPAMEIMLDDFGFGLSKRRVTLSTSGVVPA
                     LDKLGDMIDVALAISLHAPNDEIRDEIVPINKKYNIETFLAAVRRYLEKSNANQGRVT
                     IEYVMLDHVNDGTEHAHQLAELLKDTPCKINLIPWNPFPGAPYGRSSNSRIDRFSKVL
                     MSYGFTTIVRKTRGDDIDAACGQLAGDVIDRTKRTLRKRMQGEAIDIKAV"
     gene            28934..29944
                     /gene="rodZ"
                     /locus_tag="PN977_000845"
     CDS             28934..29944
                     /gene="rodZ"
                     /locus_tag="PN977_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytoskeleton protein RodZ"
                     /protein_id="HDI6040908.1"
                     /translation="MNTEATHDQNEALTTGARLRNAREQLGLSQQAVAERLCLKVSTV
                     RDIEEDKAPADLASTFLRGYIRSYARLVHIPEEELLPGLEKQAPLRAAKVAPMQSFSL
                     GKRRKKRDGWLMTFTWLVLFVVIGLSGAWWWQDHKAQQEEITTMADQSSAELNNNQSQ
                     SVPLDTSTTTDQAMATTPTSPVDTTATNTQTPAATTAPSPTVDSQQNAVVPPSQANVD
                     TAATPAPAATTMPDGAAPLPTDQAGVTTPAADPNALVMNFTADCWLEVTDATGKKLFS
                     GMQRKDGNLNLTGQAPYKLKIGAPAAVQIQYQGKPVDLSRFIRTNQVARLTLNAEQSP
                     AQ"
     gene            29971..31089
                     /gene="ispG"
                     /locus_tag="PN977_000846"
                     /gene_synonym="gcpE"
     CDS             29971..31089
                     /gene="ispG"
                     /locus_tag="PN977_000846"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HDI6040909.1"
                     /translation="MHNQAPIQRRKSTRIYVGNVPIGDGAPIAVQSMTNTRTTDVEAT
                     VNQIKALERVGADIVRVSVPTMDAAEAFKLIKQQVNVPLVADIHFDYRIALKVAEYGV
                     DCLRINPGNIGNEERIRMVVDCARDKNIPIRIGVNAGSLEKDLQEKYGEPTPQALLES
                     AMRHVDHLDRLNFEQFKVSVKASDVFLAVESYRLLAKQIDQPLHLGITEAGGARSGAV
                     KSAIGLGLLLSEGIGDTLRVSLAADPVEEIKVGFDILKSLRIRSRGINFIACPTCSRQ
                     EFDVIGTVNALEQRLEDIITPMDVSIIGCVVNGPGEALVSTLGVTGGNKKSGLYEDGV
                     RKDRLDNNDMIDQLEARIRAKASQLDEARRIDVQQVEK"
     gene            31200..32474
                     /gene="hisS"
                     /locus_tag="PN977_000847"
     CDS             31200..32474
                     /gene="hisS"
                     /locus_tag="PN977_000847"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HDI6040910.1"
                     /translation="MAKNIQAIRGMNDYLPGETAIWQRIEGTLKNVLGSYGYSEIRLP
                     IVEQTPLFKRAIGEVTDVVEKEMYTFEDRNGDSLTLRPEGTAGCVRAGIEHGLLYNQE
                     QRLWYIGPMFRHERPQKGRYRQFHQLGCEVFGLQGPDIDAELIMLTARWWRALGISEH
                     VTLELNSIGSLEARANYRDALVAFLEQHKEKLDEDCKRRMYTNPLRVLDSKNPEVQAL
                     LNDAPALGDYLDEESREHFAGLCKLLESAGIAYTVNQRLVRGLDYYNRTVFEWVTNSL
                     GSQGTVCAGGRYDGLVEQLGGRATPAVGFAMGLERLVLLVQAVNPEFKADPVVDIYLV
                     ASGADTQSAAMALAERLRDELPGVKLMTNHGGGNFKKQFARADKWGARVAVVLGESEV
                     ANGTAVVKDLRSGEQTAVAQDSVAAHLRTLLG"
     gene            32492..33112
                     /gene="yfgM"
                     /locus_tag="PN977_000848"
     CDS             32492..33112
                     /gene="yfgM"
                     /locus_tag="PN977_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ancillary SecYEG translocon subunit YfgM"
                     /protein_id="HDI6040911.1"
                     /translation="MEIYENENDQVEAVKRFFAENGKALAVGVILGVGALIGWRYWNS
                     HQVDSARSASLAYQNAVTAVSEGKPDSIPAAEKFAAENKNTYGALASLELAQQFVDKN
                     ELEKAAAQLQQGLADTSDENLKAVINLRLARVQVQLKQADAALKTLDTIKGEGWAAIV
                     ADLRGEALLSKGDKQGARSAWEAGVKSDVTPALSEMMQMKINNLSI"
     gene            33123..34301
                     /gene="bamB"
                     /locus_tag="PN977_000849"
     CDS             33123..34301
                     /gene="bamB"
                     /locus_tag="PN977_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB"
                     /protein_id="HDI6040912.1"
                     /translation="MQLRKLLLPGLLSVTLLSGCSLFNSEEDVVKMSPLPTVENQFTP
                     STAWSTSVGSGIGNFYSNLHPALADNVVYAADRAGLVKALNADDGKEIWSVNLAEKDG
                     WFSKDPALLSGGVTVSGGHVYIGTEKAQVYALNTSDGTVAWQTKVAGEALSRPVVSDG
                     LVLIHTSNGQLQALNEADGAVKWTVNLDMPSLSLRGESAPATAFGAAVVGGDNGRVSA
                     VLMEQGQMIWQQRISQATGSTEIDRLSDVDTTPVVVNGVVFALAYNGNLTALDLRSGQ
                     IMWKRELGSVNDFIVDGNRIYLVDQNDRVMALTIDGGVTLWTQSDLLHRLLTSPVLYN
                     GNLVVGDSEGYLHWINVEDGRFVAQQKVDSSGFQTEPVAADGKLLIQAKDGTVYSITR
                     "
     gene            34419..35891
                     /gene="der"
                     /locus_tag="PN977_000850"
     CDS             34419..35891
                     /gene="der"
                     /locus_tag="PN977_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HDI6040913.1"
                     /translation="MVPVVALVGRPNVGKSTLFNRLTRTRDALVADFPGLTRDRKYGR
                     AEIEGREFICIDTGGIDGTEDGVETRMAEQSLLAIEEADVVLFMVDARAGLMPADEAI
                     AKHLRSREKPTFLVANKTDGLDPDQAVVDFYSLGLGEIYPIAASHGRGVLSLLEHVLL
                     PWMEDLAPQEEVDEDAEYWAQFEAEENGEEEEEDDFDPQSLPIKLAIVGRPNVGKSTL
                     TNRILGEERVVVYDMPGTTRDSIYIPMERDGREYVLIDTAGVRKRGKITDAVEKFSVI
                     KTLQAIEDANVVMLVIDAREGISDQDLSLLGFILNSGRSLVIVVNKWDGLSQEVKEQV
                     KETLDFRLGFIDFARVHFISALHGSGVGNLFESVREAYDSSTRRVGTSMLTRIMTMAV
                     EDHQPPLVRGRRVKLKYAHAGGYNPPIVVIHGNQVKDLPDSYKRYLMNYFRKSLDVMG
                     SPIRIQFKEGENPYANKRNTLTPTQMRKRKRLMKHIKKSK"
     gene            35954..36169
                     /locus_tag="PN977_000851"
     CDS             35954..36169
                     /locus_tag="PN977_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001196900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HDI6040914.1"
                     /translation="MALNCPTCKTPLERNGGTAHCATCAKDFTIQALCPDCHQPLQVL
                     KACGAVDYFCQYGHGLISKTRVEFVIV"
     gene            36379..38553
                     /gene="sinH"
                     /locus_tag="PN977_000852"
     CDS             36379..38553
                     /gene="sinH"
                     /locus_tag="PN977_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001317981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intimin-like inverse autotransporter SinH"
                     /protein_id="HDI6040915.1"
                     /translation="MLRWTRCIMLTFISGAAFAAPEINVEHKESLPDLGSQAAQQEEQ
                     TNKGKSLKERGADYVINSATQGFENLTPEALESQARSYLQSQITSTTQSYIEDTLSPY
                     GKVRTNLSLGQGGDLDGSSIDYFVPWYDNQTTVYFSQFSAQRKEDRTIGNIGLGVRHN
                     FDKWLLGGNIFYDYDFTRGHRRLGLGVEAWTDYLKFSGNYYHPLSDWKDSEDFDFYEE
                     RPARGWDIRAEAWLPAYPQLGGKIVFEQYYGNEVALFGTDNLEKDPFAVTLGVKYQPV
                     PLIAVGTDFKAGTGDNTDLSVNATLNYQFGVPLKDQLDPDKVSAAHSLMGSRHDFVER
                     NNFIVLEYKEKDPLDVTLWLKADATNEHPECVIKDTPEEAIGLEKCKWTINALINHHY
                     KIVAASWQAKNNAARTLVMPVIKENTLTEGNNNHWNLVLPAWQYSSDKAEQEKLNTWR
                     VRLALEDEKGNRQNSGVVEITVQQDRKIELIVNNIADVPDENNHSHEASAQADGVDGV
                     VMDLDITDSFGDNTDRNGNVLPQDNLNPQLFDANDKKVTLTNKPCTTETPCVFIAKQD
                     KEKGTVTLSSTLPGTFRWKAKAAPYDDSNYVDVTFLGSDIGGLNAFIYRVGAAKPVNL
                     IGNKEPLPLNSSYRFVLWRDANKDGVFQLSEKFTEEEMKQYDYQWEFTGHSVNGNTGA
                     QANTTNADIEIPATNKDAATKFSAQVTDGVQGYGLQVNYSKK"
     gene            38609..39601
                     /locus_tag="PN977_000853"
     CDS             38609..39601
                     /locus_tag="PN977_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000802324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6040916.1"
                     /translation="MKPHMKRGLTRVALALMLAGYCAVPAAMAEDAAWVASGTTAEFE
                     GTIPWLYREGGNATINSDDADHIKVTSDGKGTRPSGSETDKRLYSGDTITLGWDIGDT
                     EGDIDDGPDGIDAKTTATIKWYSYSDNAGGGKTELTAAAGKTSYKLTDDERERYIGVE
                     IQPITQTGNPFQGTSLTLLDISTASGGGSDTDNVDPGPVVNQNLKVAIFEKGTSTNLI
                     GGNTAIALNKTYVAKLYSDENQNGKYDAGTDVDVTANYDFAWVFNGNSKQLAAAGGIA
                     NASFDNNDIVIPQTNEQARTSLNGSDRNGKTGLAIPANGDGVQGYTLSIIYKHH"
     gene            39711..47786
                     /locus_tag="PN977_000854"
     CDS             39711..47786
                     /locus_tag="PN977_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000109691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RatA-like protein"
                     /protein_id="HDI6040917.1"
                     /translation="MSVCLKPGKIIVLLGMLAAFMLSDFARAGVEWQTYPGSTGEFNG
                     TVPIADSASVPVYQGSVQLDPAASHDVAFSAKPNEFSVDDDAANLIVANPQDSEGDQF
                     STPPALRWENQTPPTVSLVWADAATPDTPLNPQPIANRSFCAQGLAGRSLVAWPQIDT
                     QQTIPLLYLLTSTGYPYEGTVALAEQKVTLNIAPAQGDLISVSASGYNETIAAAKTTV
                     GGTITLTVTTKDCQGNVAGNIPFIIKRKDAQNRQGAVNNTAPVVLGSTELTTTATEYR
                     GTSDANGTATITVTQPNGPGVKTPLVASISGIAQTSETAVIFTVLTSPDVAQATMWGH
                     MAETVEAHGYTFSRPKLAAEVSNENATVVDHNETWSTFTWSGADSHCTVLPGMRHFGA
                     LATVIPSTVQTVLGWPMQDDYYWTSLAGTTGQHHAADVSNRAEAQKPDSTTFLVSCVD
                     KPAPDVEPKIVLTPENYDDTAQAMKAKVGEDATMRLTITDNKNNDQPLAYYYFSLHLD
                     DGVNRKNQTDSAWEAHPVQITGGSNFRQVDAHTYEGMTDANGQASLTLSQPGGAGVKT
                     HITAKMRSDFNATDAKDVIFTVITSPDSDKARMWGHMRGIIESGSLYKRPLLADETEH
                     ELGTVRENNEDWALYDQNTSMQAECGVGHIPRQSSLESLFSAHPGNAIGTEYGWPTAQ
                     QSYLSAVEQETHSSVNLGNGSVDSYSGFKQNYLSCSGNEMVANVEVSTDHDVSVGTQA
                     QAKVGDTIVMTVRTINALNNSPVPFSAFTITKGMGYNRAGQVSGFDDPSSGSITMDNS
                     QYGTSQPSMVYAGTTDVRGVATVEIKQPQGVGLKTVLSVTPVNSYLPNTVNYSVIFTT
                     PTSPDVSGAQMWGHMDETITVDSSTFTRPRLAAEIASPDGTLTENNEIWARVSQANTS
                     STSKGGCGTNMLPRRSQLSALYSANSGNAVQTTHGWPTQRQPYWSSSPADVTPHFFTI
                     ALNDGAQAIGGDTPVYVSCLTTANKPASSITLEVVDKAQWNAGNNAATLKKGETLQVK
                     VTVKDAQGNALADMPFTLSRGDGYTRSGEKHIAGSGDALVAPVVVNGGLADETTLNDT
                     ATVYTAMTGSDGSKILNITRPDTHGTKTALTATLYSDATKKDSLDTIFTVVTSPDSSQ
                     AKMWGHMPETVTAEDGTVFKRPRLLKELSSQTGRTSTLEDNENWALFNINYASSSTTY
                     SGCGTNYIPTQAGLTSLFANNAGNTMKTVQGWPVATRYLSNTSDNGSMEQRNYKAVDL
                     SNGTSAAVSSTALQLLTCQTTPVTTVSQILLEAADPATLDTTYNVVKAKKGEESVVRV
                     TTKDAQGNLVGNTAFILTRANSVSRANASATMSVGSLTVTDAWGNTRNNFQSTSETIY
                     GVTGADGSTTLTLKQDNSTGLRTDLTAKLDTSSSVKATVPVVFTVVTSPDSPKANFWG
                     HMAETVAASDGSVYKRPLLLAELANTGGRQISSENGESWVRFTWNQSTDPSVSGCGVA
                     YMPTLAGLQALYDANSGNAMSTVQGWPVKATYLTNTPSDTQTGSRYYNVVQLDSGAAS
                     QITTNTGVLQTCRTTPLTAASQITLEAADPAQFVSIDSTLSAVKVQKGDSVPIRISTK
                     DAQGNFVGNTPFALKHANSINRQNVSSSQKVSVTTEVGTTVDTSATTTLYGVTGPDGT
                     TTMTLRQDASTGLRTDFYALLNDTGVSSDTLPVIFTVITSPDTPLAANWGHMAETFTS
                     SEGVTFKRPSLKAELSGGTAITVNNEVWSRLTAAEKVDASKAGCDETYQPLVNDMQGL
                     YADYPNGQLGSVLGLPTSAGYWWAYDMMMVSGNWTNQAFSPVNGQLMQASSSYTAIVM
                     CLVEPHTEAATIELTSTAQDAAKSASNGGRPSAVAKKGETIPLTVTVRDSAGNPMPYA
                     EFTLTRETTLDRSKATVNTSADDLTVTALVPANTNSVLAASGAKLIGTTGSDGKATFE
                     VSQNATTGLATPLTVTLARDTTKAATLDVIYTVITSPDSPSAKFWGHMPDTFTSSTGV
                     TFKRPLLKAETSSGSSISSNGEVWSYMSNAQNLTSTDCPLENQPRSNELLDLYSDHPN
                     GTLMTDLGLPVYAGNWWAYDMVMLSGTSWTYQLISLKDGTITQKGTTSALMLCLAQPH
                     PAGVSVTLTSSAQDDARTTSNGGRPSASAKKGNSIPIVVTVKDRDGNPLAGEAVTLKR
                     DSALSRSGTIVGTPANEIKLTELTPVSATFPLVSNGTQWLGFTGSDGTATFNVEQPDT
                     VGLAIPFNAILARDTAKVSTLDLIFTVLTSPDAALATYWGHMPDTVTAENGAVFERPK
                     LWKELPSTSGVSQITNSNEAWPVFNATQRADSNLSPCEAARRPLSDDLEALYTRYPGN
                     TITAQIGWPTQYAWWAVDNWEGGSNPQAVSLVNGSRGQYNPAHQACLVNPRASVSSVT
                     LTSTALDDATQAATVKKGDAAPIVVTVKDSAGKPVPNIAFILKRGEAVPRNSGATLYG
                     DVDAMDDLTVQPSSGAAVTLADSGNTIDGVTGADGTASFTVRQDNTPGYKTPLTVTLT
                     DNATITATLDTIFTVPTSPNVATAYFWGHMADTATVSGKTLHRPLLKSELPSGATAAA
                     TPDVNNETWALAHVMDSSKWDVAQQCGSMNNVPSSAELQTLHSGFSTLGWPSSISFPY
                     LSTDKAGSFYCGVDEGSGSLNCGIQPAKTPGFATCFQ"
     gene            complement(47840..49207)
                     /gene="xseA"
                     /locus_tag="PN977_000855"
     CDS             complement(47840..49207)
                     /gene="xseA"
                     /locus_tag="PN977_000855"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000937957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HDI6040918.1"
                     /translation="MLPSQSPAIFTVSRLNQTVRLLLEHEMGQVWISGEISNFTQPAS
                     GHWYFTLKDDTAQVRCAMFRNSNRRVTFRPQHGQQVLVRANITLYEPRGDYQIIVESM
                     QPAGEGLLQQKYEQLKAKLQAEGLFDLQYKNSLPSPAHCVGVITSKTGAALHDILHVL
                     KRRDPSLPVIIYPTAVQGDDAPGQIVRAIELANQRNECDVLIVGRGGGSLEDLWSFND
                     ERVARAIFASRIPVVSAVGHETDVTIADFVADLRAPTPSAAAEVVSRNQQELLRQVQS
                     ARQRLEMAMDYYLANRTRRFTQIHHRLQQQHPQLRLARQQTMLERLQKRMSFALENQL
                     KRAGQHQQRLTQRLNQQNPQPKIHRAQTRIQQLEYRLAETLRAQLSATRERFGNAVTH
                     LEAVSPLSTLARGYSVTSAADGAVLKQVKQVKVGETLTTRLGDGVVISEVSAVTKSRK
                     PRKKA"
     gene            49369..50835
                     /gene="guaB"
                     /locus_tag="PN977_000856"
     CDS             49369..50835
                     /gene="guaB"
                     /locus_tag="PN977_000856"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004151980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HDI6040919.1"
                     /translation="MLRIAKEALTFDDVLLVPAHSTVLPNTADLSTQLTKTIRLNIPM
                     LSAAMDTVTEARLAIALAQEGGIGFIHKNMSIERQAEEVRRVKKHESGVVTDPQTVLP
                     TTTLREVKELTERNGFAGYPVVTEENELVGIITGRDVRFVTDLNQPVSVYMTPKERLV
                     TVREGEAREVVLAKMHEKRVEKALVVDDEFHLIGMITVKDFQKAERKPNACKDEQGRL
                     RVGAAVGAGAGNEERVDALVAAGVDVLLIDSSHGHSEGVLQRIRETRAKYPDLQIIGG
                     NVATAAGARALAEAGCSAVKVGIGPGSICTTRIVTGVGVPQITAVADAVEALEGTGIP
                     VIADGGIRFSGDIAKAIAAGASAVMVGSMLAGTEESPGEIELYQGRSYKSYRGMGSLG
                     AMSKGSSDRYFQSDNAADKLVPEGIEGRVAYKGRLKEIIHQQMGGLRSCMGLTGCGTI
                     DELRTKAEFVRISGAGIQESHVHDVTITKESPNYRLGS"
     gene            50904..52481
                     /gene="guaA"
                     /locus_tag="PN977_000857"
     CDS             50904..52481
                     /gene="guaA"
                     /locus_tag="PN977_000857"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HDI6040920.1"
                     /translation="MTENIHKHRILILDFGSQYTQLVARRVRELGVYCELWAWDVTEA
                     QIRDFNPSGIILSGGPESTTEENSPRAPQYVFEAGVPVFGVCYGMQTMAMQLGGHVEA
                     SNEREFGYAQVEVVNDSALVRGIEDALTADGKPLLDVWMSHGDKVTAIPSDFVTVAST
                     ESCPFAIMANEEKRFYGVQFHPEVTHTRQGMRMLERFVRDICQCEALWTPAKIIDDAV
                     ARIREQVGDDKVILGLSGGVDSSVTAMLLHRAIGKNLTCVFVDNGLLRLNEAEQVLDM
                     FGDHFGLNIVHVPAEDRFLSALAGENDPEAKRKIIGRVFVEVFDEEALKLEDVKWLAQ
                     GTIYPDVIESAASATGKAHVIKSHHNVGGLPKEMKMGLVEPLKELFKDEVRKIGLELG
                     LPYDMLYRHPFPGPGLGVRVLGEVKKEYCDLLRRADAIFIEELRKADLYDKVSQAFTV
                     FLPVRSVGVMGDGRKYDWVVSLRAVETIDFMTAHWAHLPYDFLGRVSNRIINEVNGIS
                     RVVYDISGKPPATIEWE"
     gene            52674..53924
                     /locus_tag="PN977_000858"
     CDS             52674..53924
                     /locus_tag="PN977_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020231273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDI6040921.1"
                     /translation="MLTDTKLKNLKPQDKLYKVSDRDGLYVAVLTSGTVSFRYDYRIN
                     GRRETLVIGQYGRDGISLAEAREELIAAKKLLKAGQSPAAAKRDGIKKIRGAETFAVH
                     TDSYMKHVILADSTRAMKQAVIDRDILPVLGNKMMAEITTSMVRDLCDRIVERGGRAT
                     AVQAREIISSVYRHANDRGHGLFNPAADIKPSSIAIFKPRERTLTPEEIGLFFRTLDA
                     IGAMGTMKMALKLVLITMVRKGEFTNATWDEIDFKKWTWTIPSDRMKGSRAHVIYLPK
                     QAQDILVGLQMCAGGSEYLVPGRYNFRKPLSNAALNSLIDRTVKIINEDGEHIQDFTV
                     HDMRRTASTLLHEAGYPSDWIEKALAHEQKGVRAVYNKAEYARQRAYMLQQWSDMIDS
                     WINGEHTDLIPFSPSKFEKWMAGE"
     gene            complement(53956..54612)
                     /locus_tag="PN977_000859"
     CDS             complement(53956..54612)
                     /locus_tag="PN977_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001555611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor Ant"
                     /protein_id="HDI6040922.1"
                     /translation="MTSQLIPVFNGTISNETALLCNARDLHAFLGVKKVFAAWITNRI
                     SEYEFIENQDYILLSNLGKQTSGRGGHNRKEYHLTLDTAKELAMVERNEKGRQVRRYF
                     IECEKRLRQQETKVEKVLSGFMPAIMEAIKLEDKKEYSAPLKPGYRSLIHSPSGVLGL
                     TENSLLMNLLNQLQEDGHDVSGAAAELTTMFCYIVGVSKCLRDIQTHAEYINDKAGFF
                     "
     gene            complement(54937..55185)
                     /locus_tag="PN977_000860"
     CDS             complement(54937..55185)
                     /locus_tag="PN977_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001555198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6040923.1"
                     /translation="MKQIMVAHNGVRFEVVMVLTKYRNDEVLQRKETVTYRYKHRSSA
                     VRRLRKITEELKNDPHRVIEVKGNPYKITTSVELRHMQ"
     gene            complement(55182..55832)
                     /locus_tag="PN977_000861"
     CDS             complement(55182..55832)
                     /locus_tag="PN977_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine methylase"
                     /protein_id="HDI6040924.1"
                     /translation="MTKKYTLIYADPPWVYRDKAADGNRGASFKYPVMSVMDICRLPV
                     WDLADENCLLAMWWVPTQPLEALKVVEAWGFRLMTMKGFTWIKCGSRQPDKLVMGMGH
                     MTRANSEDCLFAVKGKLPPRINAGIVQSFTAPRLEHSRKPDVVREKLVQLLGDVSRIE
                     LFARQTSHGFDVWGNQCEDPAVQLHPGYALDIGGLTNAFSNAPVSPTDNQGRERAA"
     gene            complement(55825..56076)
                     /locus_tag="PN977_000862"
     CDS             complement(55825..56076)
                     /locus_tag="PN977_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001429704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
                     /protein_id="HDI6040925.1"
                     /translation="MRSPSKVVAVTPAAIEIEKRAIEREKKGQFRIAAHLWLQCMDVA
                     SGDVERARIAVRRDQCITKGNGLRRGDYSGIGCCGVVYD"
     gene            complement(56234..56482)
                     /locus_tag="PN977_000863"
     CDS             complement(56234..56482)
                     /locus_tag="PN977_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009485476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family phage regulatory protein"
                     /protein_id="HDI6040926.1"
                     /translation="MKGALGKKELLAVVPLSWSTIDRMERAGEFPKRWYITDKRCAWN
                     RDEVERWLDERQAASPAEFQGKKPPVQQRVYRPVSNAA"
     gene            complement(56532..57413)
                     /locus_tag="PN977_000864"
     CDS             complement(56532..57413)
                     /locus_tag="PN977_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000063822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecT"
                     /protein_id="HDI6040927.1"
                     /translation="MSNDIAITSQPGATVGTAAAIFSPEGMNQLVRFAELMSQSKATV
                     PKHLEGKPADCLAVTMQAAQWGMNPFAVAQKTHVVNGTLGYEAQLVNAVVSSSSLLAT
                     RLNYRWSGDWSNVNGKTDKSPNLTVTVSAVLKGEAEPRELTISMAQAGVRNSPLWEQD
                     PRQQLAYLCTKRWARLHAPDVLLGVYTPDELQETAPRVERDITPQMTTAAGMNSLINA
                     KSVKKPDEQTRKADSREPEEMLMAFTSAAMNYSTVSELDKAYKYIAQKLSDDDELLAK
                     ATDVYSVRREELNETSM"
     gene            complement(57410..58231)
                     /locus_tag="PN977_000865"
     CDS             complement(57410..58231)
                     /locus_tag="PN977_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000802265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease-like domain-containing
                     protein"
                     /protein_id="HDI6040928.1"
                     /translation="MKPGIYFDISNEDYHAGDGVSKSQLDMVAKNPALLKWVQAAPED
                     EEKKSALDMGTALHCLLLEPGEFDKRFIVSPKFDRRTKQGKADEEAFLRDVADMGITV
                     LDAEQWRKLELMRDSAMAHPAARWMLEAPGYCEASMYWNDEETGELCRIRPDKWLKEH
                     NVIVDVKKVTDMDRFARHIEEFRYHVQDAMYREGAMRVTGQPHGFFFLAVSESIDCGR
                     YPVRVFELDAPDVDAGHALFRRDLNTYHECRINDEWGGVEIIKRPDWARKQDMYV"
     gene            complement(58228..58527)
                     /locus_tag="PN977_000866"
     CDS             complement(58228..58527)
                     /locus_tag="PN977_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005028273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6040929.1"
                     /translation="MNVNQQKNLQKIMLAFDKDYRLSEQLYDRQVELIESIRLHQLAS
                     TFDVVTGKGVRQEVLEAAKDSPEFEELMDSYRREAMAIIARWDLADQLDGQRDAA"
     gene            complement(58530..58682)
                     /locus_tag="PN977_000867"
     CDS             complement(58530..58682)
                     /locus_tag="PN977_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001198620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6040930.1"
                     /translation="MQWKIINGWYCVTACGFMSWKFRTLQEGIKWAFVSKEARDVAND
                     NEIWEG"
     gene            complement(58836..59420)
                     /locus_tag="PN977_000868"
     CDS             complement(58836..59420)
                     /locus_tag="PN977_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000836290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDI6040931.1"
                     /translation="MKSIQDVRRQNLNDLIDREFNGVQTRMAEKLGTQANLVNRWALG
                     KKVIGDQVARKIEAAANKPRNWLDIDRSLSQEGFQPVGPSDIGQLAAHNLERWMSESR
                     DLSTQGKLHRASGVAQVTISRLLNNEVSVSISTLENVASAFGRHGYELLIHPHDPATI
                     NYDRSRYALLPETEKAKIESYIEFVINQNEKSKQ"
     gene            59575..59805
                     /locus_tag="PN977_000869"
     CDS             59575..59805
                     /locus_tag="PN977_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001282459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6040932.1"
                     /translation="MSDNGHFDFKKHWLALTPDEREAFAQEAGTTSHYIQTHLTGKRK
                     MPGKVLMNGLFKACKTRQWLRSKAELAYFFYS"
     gene            60147..60977
                     /locus_tag="PN977_000870"
     CDS             60147..60977
                     /locus_tag="PN977_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000168728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6040933.1"
                     /translation="MASSWIKVEVITPDKPEIFQIAEILGIDPDAVLGKLVRIWAWAD
                     QQTIDGNAGSVTKGVLDRLAFITGFADALISVGWLAYHDGKLILPNFERHNGESSKKR
                     ALTNRRVAEHRKRVTQKVTPTALQKELPEEEEEEDIYKTPHIAHVREGAPTSEANSTP
                     LQVAEPEFLDGLSEPIGKFPMTDGWLPSPDFRRRAALWGTALPEPEFTPAELAAFRDY
                     WMAEGKVFTQVQWEQKFARHVNHIRGKSKNAGKSDELDWNNTDWIEGVWDEINSRTSQ
                     "
     gene            60949..61725
                     /locus_tag="PN977_000871"
     CDS             60949..61725
                     /locus_tag="PN977_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020231269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HDI6040934.1"
                     /translation="MKSTPELLNEYDRLREHGVAVHEERRDSNGKKEQVARIFNELFV
                     QLQAAFPASVSTIREQNKLNEFRKQWMLAFLENGITTMEQVNAGMRHARASESPFWPS
                     PGQFIKWCKDSKMVLGVTIDDVMAEFHRYSKEKSLYPGGPERFPWRHPVMYWVVCDTR
                     RAMYQRQLSEIEVEKHARRLLDDWAKKVASGQQIPDPVISIQAKPEPMSTPPDTGRDV
                     YHPPGRSFGCMPNAATLGGITPAQWLIEEYRRGKAAGFIK"
     gene            61843..62187
                     /locus_tag="PN977_000872"
     CDS             61843..62187
                     /locus_tag="PN977_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001550162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1064 domain-containing protein"
                     /protein_id="HDI6040935.1"
                     /translation="MRKQIQALGRLKTGQMNKTESAYCQHLELRKRAGEIVWYRFEGI
                     KLRLADNTFYTPDFAVMLATGEMELHEVKGFWTDDARVKTKVAADQYPFRIIGVTVKP
                     KKAGGGWNIEEF"
     gene            62249..>62403
                     /locus_tag="PN977_000873"
     CDS             62249..>62403
                     /locus_tag="PN977_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000445433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6040936.1"
                     /translation="MESNIKGLVAAGHEMASELKAECGAVDMRSVAKLISDLATQLEV
                     QLVRANAL"
BASE COUNT        15159 a        15590 c        17071 g        14583 t
ORIGIN      
        1 caagcaacgg cagcggtctg gctgccgcgc cggttgctat aaaaagctga tcccagtgcc
       61 agctttcgcc gttagctaac actaactctc gtgtgtcgcg gcccaatgtt ttgatggtta
      121 caccggaatg cagatgaaca ttgttttcct gccaccagtg agcgggtaac acagactgca
      181 actgtgggga atcttccagc aacatggatt tcgagagcgg ggggcgttca taaggaagat
      241 gttgctcatc ggaaaacaga tgcagctcac cggtgaaccc ttgctggcgt agcgaggccg
      301 cagccattgc cgccgcttgc ccgccaccga caataatgat cgttttttct ttcatcgtgc
      361 ttccttaaag atccagccca gccgctacgt ggcgaatgcc gcgaatcgct aaacccgcat
      421 cagcgttgat catcacaccg cttaatgcgc gattattgcg ccgcgatgcc aacatcacat
      481 acggacccgt aaaatccgcc ggttgcggga aaaattgcag cggtaatatg gcggcaattt
      541 tctccggcgt cagagactgc attatcgagg tttcactttg cccgagcgcc tgtgggccgc
      601 gcaggtcgct ggccatacca cacgggccga cgccattcac ccgcactttc ggtgccagtt
      661 cataagccag ttggcgaata agtccggttg cggcatgttt actggcggtg tacagcgggc
      721 caccgccgcc gggataccag gcggcatttg acagtgtgaa aatcatgctg ccttcactgg
      781 cgattaacgc cggagcgcag gcttttgcgc ccagcaggta accgagtacg ttgacgttaa
      841 acagctcgtg gaagccggtt tcgagcgtct ctgcgggagt attaaccagt gaggcattgt
      901 gatcccagat gcccgcattg ccgataaaac aatccagttt gccagaacga gtcaggatct
      961 gatcgagcgc gcgttgataa tcggcataac aggtcacgtt gccttccacc gccagaatat
     1021 gttcaccaaa tcgctgacgc agactggcga ctttcgccgc cgacagttcc agcgtggcaa
     1081 cctgcgcgcc ttcttcgata aatcgctcga ccagcgccag gcctaatccc gatccgccgc
     1141 cggtaataaa gatggactcg ttatgcagat cgctcatggc tgcgcctccg gtaagtcgat
     1201 gaagatgtca ccaccttcaa cgtgtactgg ataagtggtt agcggatcgg tggcgggcag
     1261 gcataatgct ttccccgttt tcaggcaaaa actggcggcg tgcagcgggc actccaccgt
     1321 ggcgtcatct tccagatacc cttctgacat cgacgcatta ccatggctgc aacgatcgtt
     1381 aatggcataa aactcgccgc ctacgttgaa cagggcgatg acgggcgagg tatcgatccg
     1441 gagagcctca ccctccggca catccgcaac gggacacgca taaattcgat tcatcagaac
     1501 agtacactca ggttatggga agtgatcacc gcttgatcca ggacgatatc ccgttccagc
     1561 aagcgccagt tgtcatcttc cagacggcga actttgtcga aacgcgttcc aacatagaat
     1621 gtttcatcgc gctctttttg tgcccgataa agtagataat tcacccgcac agcaaatacg
     1681 tttgggatgt cggtttcgct tacctggcag ttgctgatta agtgacgagt gcgtgatggc
     1741 ggctcttctg cccaggccat gcccgtttcc agacgggcga ttcgccgctc cagctggtct
     1801 ttagtgtcat taaaaatcca ggtcgttggt ggctggacgc ctttgcggcg gtcgcgtgtt
     1861 tgcgcgttaa ctgtggtgcg catggtgtaa cgaatttctt cgtcgagctg tgccagccag
     1921 tcacgaaatt tccagtcatc cagcagactg gcttcgtgaa agagaaactg gctaatgcgg
     1981 tgatgtaact ccagtgaaac ttgcgcactc atttcatcac ctcctgctgg taagcggcgg
     2041 ttttatcgag cacttcctgc cagctttcgc tactcagaag atcggcccag cgttggtaca
     2101 ttccacgagc ggccgtttct gaaaagatat agttagtaat gccaggaatg ccgtcgtcgc
     2161 ggtgcttttc ctgaccaagc cccatttcca gacacagttt gctgttgcgg gcgcggtggc
     2221 ctttaagcaa tttctggatt tcacaccagt tctccgagtc atcctgctcg agaaaaccag
     2281 caggaccaaa agcacgagtg gcgctgtttt caaaagcggc tttaacttca tcggaggcag
     2341 ctttgtcagt aatacagaac gcccacactt cgacttgatc agggccgcgc ggatgccaga
     2401 cgcggagcgt ggcagtgccg ttgagccatg aaagcgtggg gaaaatattg ttatgacccg
     2461 ccaggcgcag ggcacgaact tcacctaaac gttgttctgc ttcggcatag gtttcgcggt
     2521 aatagctgga aactgcgcca tcgacccaga cattagcatc cggtttttca gtaaagaaga
     2581 aaccgctgcc gtgaccgtcc tgaccaaact gcagcgcatc tttggcggtt tcccacaccg
     2641 ggcgggcagt ttgtccatca ccgagacgct tatcgctgcc atcatctttc gcccctaata
     2701 cctgaacggc agaagcatgg ctgaacagag catgatactg gtcactggcg aactgctctg
     2761 ccgggaattt ccagttgcag ttgatcaccc acttttgtac gccgccgaca atttcggtgc
     2821 cgccttcgcg acgatccagc atgccatcca gataccaggc aatgtcacca aggtaatcac
     2881 gcaggcccgg tgcgctggta tcccagttgc caaaaatcag ccctttataa ctctccacac
     2941 aaggaacttc gtttagtccc cagtgggatt tacacaaccc ttgtgggtag gcgcgaggtt
     3001 ccagcggtac atcgatcaac tcgccgttaa tgccataaga ccagccgtga tacggacagg
     3061 taaaggcgcg agtgttgccg caatctgcat aactcacacg catggcccgg tggcggcact
     3121 ggttgagaaa cgccttgatg ctgccgtctt tctggcgcac cacgacaact gcatcttccc
     3181 ccatgtaggt gttaaagaaa tcaccaggtt ttgggatctg gctttcgtgg gcgagaaata
     3241 gccagcaacg accgaaaatg cgctcaagct ccagttggta aatgtccggg tcggtataaa
     3301 tacgcggagt gacccgaccg ttttgggtgt caatcagttg gtaaatgttc aaatctgagg
     3361 gtgtggtcat attttttaat cctgaaagat aagatgtatt gaccttctat ttttgtgcca
     3421 taaggatgtt gctggttggt tgctgatatg tgaaatagtt aatttctacc agctgagacg
     3481 ttttttcgat aacaagggga aggtgatgga actacggcat ttacgctatt tcgtcgcagt
     3541 ggcgcaggca ctgaacttta cccgtgcggc ggaaaaactg catacctcac agccttcgtt
     3601 aagcagccag atccgcgatc ttgaaaactg tgtcggtgtt ccgttactgg tgagggataa
     3661 gcgcaaagtc gcgttgacgg cggcgggaga atgttttctc caggacgcgc tggcaatcct
     3721 cgaacaagcg gaaaatgcca aattacgggc gcggaaaatt gttcaggaag acagacaatt
     3781 aaccattggc tttgtgccat cggcggaagt gaatttactg ccaaaagtat taccgatgtt
     3841 tcgtctcaga cagccagaca ccttaattga gctggtgagt ttaatcacca cggaacagga
     3901 agaaaaaatt cgccgtggtg aactcgacgt cggcttaatg cgccatcccg tttatagccc
     3961 ggagattgat tatctggagc tttttgacga accattagtg gtggtgttac cggttgatca
     4021 ccctttagca catgaaaaag agatcactgc cgcgcaactg gatggcgtga atttcgtcag
     4081 tactgatcca gcgtattccg gttcgcttgc gccgatcgtt aaagcgtggt ttgcgcaaga
     4141 aaacagccag ccaaatatcg tccaggtggc aacgaatatt ctggtgacca tgaatctggt
     4201 ggggatgggg ctgggcgtca ctttgatacc cggttatatg aataatttta ataccggcca
     4261 ggtggtattt cgacccattg ccggaaatgt gcctgccatt gctttactga tggcgtggaa
     4321 gaaaggggag atgaagccgg cattacgcga tttcatcgcc attgtgcagg aacgtttggc
     4381 aagcgtaacg gcataattac gcattatttt tgcaacatgc gtcgcggatc gcagtttttt
     4441 agtgcttgtg aactggtgtt acagacatcg ggaagcaggc tcgaatattc ttatttttca
     4501 acttgcacca acattcgatt aaccccagac taacgcctcc tgacgggagg gactcatggt
     4561 tttgcaatcc acgcgctggt tggcgctcgg ctatttcaca tactttttta gttacggcat
     4621 ttttctacct ttctggagcg tctggcttaa aggtattggt ttaacgccag aaaccatcgg
     4681 cctgttattg ggggcgggtc tggttgcccg tttcctcggg agtttgctca tcgcgccccg
     4741 cgtcagcgat ccttcccgcc tgatttccgc cttgcgcgtg ctggcactgc tgacacttct
     4801 ctttgctgtc gccttctggg cgggggcgca cgtagcgtgg ctgatgctgg tgatgattgg
     4861 ctttaacctc tttttctcac cgctggtacc gttgaccgat gcactggcga atacgtggca
     4921 aaagcagttc ccgcttgatt acggcaaagt gagactgtgg ggctcggtag cgtttgtcat
     4981 tggctcggcg ctgacgggca aactggtcag tatgtttgat tatcgggtga tcctcgcgct
     5041 gttgacgttg ggcgtggcat ccatgctgct cggctttctc atccgtccga cgattcagcc
     5101 acaaggggca agccgccagc aggagagcac cggttggtct gcgtggttgg cgttggttcg
     5161 ccagaactgg cgctttctgg cctgcgtttg tttattgcag ggggcacatg cggcctatta
     5221 cggttttagc gccatttact ggcaggcagc tggctactcg gcctcggcgg tgggctattt
     5281 gtggtcgctg ggcgtggtgg cagaagtcat tatctttgcg ctgagtaata agcttttccg
     5341 ccgttgtagc gcccgtgata tgttgctgat ctcggcagta tgtggggtat tgcgttgggg
     5401 aattatgggg gcaaccactg agctactgtg gttgattatg gtgcaaattt tgcattgcgg
     5461 caccttcacc gtgtgccatc tggccgctat gcgctacatt gctgctcgcc agggtagcga
     5521 agtcatccgt ttacaggcgg tttactctgc cgtcgcgatg ggcggcagta tcgccatcat
     5581 gaccgttttc gccggtttcc tgtatcaata tctcggccac ggcgtgttct gggtgatggc
     5641 gctggtggcg cttccggcaa tgtttttgcg cccgaaagtt gttccctcat gctgattcga
     5701 gcattttgcg gatctgctcc tgttgatgtg ttgtcaacgt caggtccgta tagatcagcg
     5761 gtggtgaaaa gagcggtaac ggcatggtat aaggagcaat aatcagtgcc gcgccgcgcg
     5821 gagcgcctgt ctgcaaaaat gcctttaccg acatatgttt aatgttgagc ggcagtaacg
     5881 ttagttcgcg tagctgctgc tcaatctgcg cttctcgctc gctatcattc ccggtcagta
     5941 gaataatctg tttctcatgc aggtcgtttt cctgcattag ccaggcaccg aaaatcaccg
     6001 cgaccagacc gctttcctca tcagaaagat ggacgccgta ttcactttca aatccggcca
     6061 gcgccgcgcg ggtggtgcgc accaggcgtg gatacaggcg ggcgaactct tccggcaggg
     6121 tgttatcaat accgatggca aacaaactgc gatttaacgc ctgggcgagg tgggtataaa
     6181 gctgatcgca caacccctgt tcatcataaa aacgaacatt tcccagctca cgaaaatggt
     6241 ttaccagacg tttgatagac tgacgcagtt gtctgtcccg ctgatgcgca tcccgcagtg
     6301 gatcgggaac ccgcaacatg gaaaaaagta atgccatgaa cagtggctca tcaggcggta
     6361 caggctggag cgcccgacgc tgccagtggc gtccaatttc ctgcgctacc tgaaattcaa
     6421 ggcaggattc cgcccagcga cgttggagcg gattaaactg aggcgtaatt ccagcgtggt
     6481 gttgcagcaa acaatattgc agatacagac acaggaaatg aatatcgcgc gtttcaaagc
     6541 gtttttgcag ccgtcgggag cagagattca ctaacgcttg tagattggta tcgtcgtaaa
     6601 agttacgcgc aattccgcgc cgcttcagtt cactcttcag ggcgggggtg aaatggctgg
     6661 taataaatga cggacacaga cggaaaccac gtcgtagcca gtgaaataaa cacaaacgtt
     6721 ggttaagcac tgtaccttca acccggtagc taccgtcata accagtagtc agtgtgagtt
     6781 gatgatagcg caggatctcc cgtcctgttt cgctgatatc aagactggca atatcatcat
     6841 ccacaccatt aagctcgctg aaagttgccg tggtggcggt caaccccggc tgaaagagtg
     6901 tcagcaagat ctggcagcgg cgctggggag ccgaaaggac agatggtgga gcaagcgtag
     6961 gcatcatctg tagatctccc ataagcataa ttgataagaa tagctaaagt ttgctcaggg
     7021 aaatggcgaa gggaatgcta atcatcagaa atgttggcaa gcgtcacaga atttttctca
     7081 tcttaaactg ttctgaccag tggattagca tgtcttaagg tggttttgtg aatgattatc
     7141 gtgctagcga tcatgggtta gttctgcgga ttcaacgcgc taaggataaa atccaccatc
     7201 tgttcgcgat aaacatcgcc gtgacgatca gaaaaagcat caatgtgttc gccatccggg
     7261 attaggatca accgttttgg ctcttttgcc aggctataca acttttcgct gtgctgccat
     7321 gggataacgt gatcagcttt accgtgaatg agtaaaagcg ggatcgggct gacgctggcg
     7381 atgtaatttt cgccgctgta actctcatca agtaagtagc cactgccggg gatcatctgg
     7441 ttggcgatgg tcgcataaga ggcaaatgtg gagtcgagga tcaccgcacg tatgccttca
     7501 cgatcaccct gaccaataac atcaagaata ttcgccccgc caatactctg cccgaacagc
     7561 accagccgtt gtgggtttac atcactgcga tggcgcgcca cattgatggc actttgcgta
     7621 tcgtctaata accctgcctg agaaggtttt ccttttgatt taccaaaccc gcgataatca
     7681 aacataaaaa cgttgaaatt acgctcgggt aaccaactga ccagcggcca gtgggcggac
     7741 atatttccgg cattgccgtg agcatgaata atggtagcaa tggcgttatc agcagggccc
     7801 gtcgaagaag gaataaacca gccttgcaga cgagtaccgt ccttagccgt aaattcgacg
     7861 gattccgccg accaggggtc gggaccgtat attttatcgt caggataata gaagaagttg
     7921 atggcgacgc gcggcactaa ataaacgcag aaggcaacaa caaagagaat aaacagaatt
     7981 ttgagaacgc gtttgttcac tggcagcgcc ataacctttc cttgcgactt ctttatcatc
     8041 cattaatcgg ggaaagatgg tgggagagtg acgaaagaaa gtaaagcggt gaggaagtat
     8101 ttcggaatag cgcctgaaag gcgagggcgg gtgagtgaca tcacccgcca gagtcattaa
     8161 cgcttcaggg cgtcgcttaa ctcgtcacgc atattcgcca gcatggcttt cacaacgcgc
     8221 gggttaccag caacgatgtt accggtcagc atatagttat gaccaccggt gaagtcgctg
     8281 acgatgccgc ccgcttcacg aaccagcagc tcgcctgccg cgaaatccca cgggcgcaga
     8341 ccgatttcaa agaaaccgtc aacacgaccc gcagcgacgt aagccagatc cagcgccgca
     8401 gaaccggtac gacggaagtc tgcacattcg ttgaacagtt tgccgacgat gttgatgtag
     8461 gtagtggcgt actgttttgc tttgaacggg aagccggtcg ccagaatagt accgtcgaga
     8521 tcgcgagcgg tgctgccgcg cagacggtag ccgttcagct gtgcgccctg accgcgagtg
     8581 gcggtgaaca gttcgttacg cataggatcg tataccacag caacttcggt gcggcctttg
     8641 atacgaacgg cgatagatac cgcgaagtgc ggcagacgtt tgataaagtt ggtagtgcca
     8701 tccagtggat cgataaccca ttgaacatcc tgatcggtac cttcaagttc accgctttct
     8761 tcggtgatga tggtgtgctg tgggtaagat ttacgaatcg tgtcgataat caccgcttcg
     8821 gcagctttat ctacgttggt cacgaaatcg ttactgcctt tctggctcgc ttctacagcg
     8881 tccggggttt catagttttt ggcaattaaa ttacccgcct tgcgcgctgc gcgcacggcg
     8941 atgttcagca tcggatgcat cggtctctct cactggatgt taaagaacgg gaaaacggcg
     9001 cagagtatag cagcgagatc ggcatctgtc ttcgttttat gataagatgc gcgaataatc
     9061 tttagacgaa gaaagacaat gctgcaaaat attcgaattg tgctggtgga gacgtcacac
     9121 accggcaata tgggttctgt tgcccgtgcc atgaaaacaa tgggattaac caatctgtgg
     9181 ctggttaatc cactggtgaa acctgactcc caggcgattg ccctggcagc aggggccagc
     9241 gatgtgattg gtaatgctca catcgtcgat acgctcgacg aagcgttggc tggttgtagc
     9301 ctggtggtgg gcaccagcgc acgttcccgc acgctgccgt ggccgatgct cgacccgcgc
     9361 gaatgcggtc tgaaaagcgt cgctgaagcg gcaaataccc cggtggcgct ggtttttggt
     9421 cgtgagcgtg ttggcttgac caatgaagag ttgcagaaat gccattatca tgtcgcgatt
     9481 gccgcgaacc cggaatacag ttcgctgaac ctggcgatgg cggttcaggt tattgcctat
     9541 gaagtgcgca tggcctggct ggcaactcag gaaaatggcg agcaggtcga gcatgaagag
     9601 acgccgtatc cgctggtcga tgatctggag cgtttttatg gtcatctgga gcaaacgctg
     9661 ctggcaaccg gttttatccg tgaaaaccat ccggggcagg tgatgaataa attgcgccgt
     9721 ctgtttaccc gtgcacgccc ggaaagccag gaattgaata tcctgcgcgg gattctggct
     9781 tctattgagc agcagaataa aggtaacaag gccgaataac agtggttgcc tgatgtgatg
     9841 cttaacgcgt cttatcaggc ttgtaagtgc acggaatccc aggtcggata aggtgttcac
     9901 accgcatccg acaacaggta caaacgccac gataaaaaaa tggcactgaa ggttaaatac
     9961 ccgactaaat cagtcaagta aatagttgac caatttactc gggaatgtca gacttgaccc
    10021 tgctatgcaa tacccccact tttacaataa aaaaccccgg gcaggggcga gtttgaggtg
    10081 aagtaagaca tgagactgac atctaaaggg cgctatgccg tgaccgcaat gcttgacgtt
    10141 gcgctcaact ctgaagcggg cccggtaccg ttggctgata tttccgaacg tcagggaatt
    10201 tccctttctt atctggaaca actgttttcc cgtctgcgta aaaatggtct ggtttccagc
    10261 gtacgtggac caggcggtgg ttatctgtta ggcaaagatg ccagcagcat cgccgttggc
    10321 gaagtgatta gcgccgttga cgaatctgta gatgccaccc gttgtcaggg taaaggcggc
    10381 tgccagggcg gcgataaatg cctgacccac gcgctgtggc gtgatttgag cgaccgtctt
    10441 accggttttc tcaataacat tactttaggc gaactggtta ataaccagga agtgctggat
    10501 gtgtctggtc gtcagcatac tcacgacgcg ccacgcaccc gcacacaaga cgcgatcgac
    10561 gttaagttac gcgcttaata aaaagaattc agaatcaggc cggagtgcta aacactccgt
    10621 aaacacggtc gtacatccag ccggtagcct ggttccttgc attgagtgat gtacggagtt
    10681 tatagagcaa tgaaattacc gatttatctc gactactccg caaccacgcc ggtggacccg
    10741 cgtgttgccg agaaaatgat gcagtttatg acgatggacg gaacctttgg taacccggcc
    10801 tcccgttctc accgtttcgg ctggcaggct gaagaagcgg ttgatatcgc ccgtaatcag
    10861 attgcagatc tggtcggcgc tgacccgcgt gaaatcgtct ttacctctgg tgcaaccgaa
    10921 tctgacaacc tggcgatcaa aggtgcagcc aacttttatc agaaaaaagg caagcacatc
    10981 atcaccagca aaaccgaaca caaagcggta ctggatacct gccgtcagct ggagcgcgaa
    11041 ggtttcgaag tcacctacct ggcaccgcag cgtaacggca ttatcgacct gaaagaactt
    11101 gaagcagcga tgcgtgacga caccatcctc gtttctatca tgcacgtgaa taacgaaatc
    11161 ggcgtggtgc aggatatcgc ggctatcggc gaaatgtgcc gtgctcgcgg cattatctat
    11221 cacgttgatg caacccagag cgtgggcaaa ctgcctatcg acctgagcca gttgaaagtt
    11281 gacctgatgt cattctccgg tcacaaaatc tatggcccga aaggtatcgg tgcgctgtat
    11341 gtgcgtcgta aaccgcgcgt acgcatcgaa gcgcaaatgc acggtggcgg tcacgagcgc
    11401 ggtatgcgtt ccggcactct gcctgttcac cagatcgttg gtatgggcga agcctatcgc
    11461 atcgcaaaag aagagatggc gaccgagatg gaacgtctgc gcggcctgcg taaccgtctg
    11521 tggaacggca tcaaagatat cgaagaagtt tacctgaacg gtgacctgga acacggtgcg
    11581 ccgaacattc tcaacgtcag cttcaactac gttgaaggtg agtcgctgat tatggcgctg
    11641 aaagacctcg cggtttcttc aggttccgcc tgtacgtcag caagcctcga accgtcctac
    11701 gtgctgcgcg cgctggggct gaacgacgag ctggcacata gctctatccg tttctcttta
    11761 ggtcgtttta ctactgaaga agagatcgac tacaccatcg agttagttcg taaatccatc
    11821 ggtcgtctgc gtgacctttc tccgctgtgg gaaatgtaca agcagggcgt ggatctgaac
    11881 agcatcgaat gggcacatca ttaatcggta tcggaatcag gagaatttat aatggcttac
    11941 agcgaaaaag ttatcgacca ttacgagaat ccgcgtaacg tgggttcttt tgacaacaac
    12001 gacgagaacg tcggcagcgg catggtgggt gcaccggcct gtggcgacgt gatgaagttg
    12061 cagattaaag tcaacgatga aggtatcatt gaagacgcgc gttttaaaac ttacggctgc
    12121 ggttctgcta tcgcttccag ctccctggtc accgaatggg tgaaagggaa gtctctcgac
    12181 gaagcgcagg cgatcaaaaa caccgatatt gctgaagaac ttgaactgcc gccagtgaaa
    12241 attcactgtt ctattctggc agaagacgcg atcaaagccg ccattgcgga ctataaaagc
    12301 aaacgtgaag caaaataaga gttgaggttt ggttatgtcg attacactga gcgacagtgc
    12361 agcagcgcga gtaaatacct ttctggctaa ccgtggcaaa gggtttggcc tgcgtctggg
    12421 cgtgagaacc tccggatgtt caggtatggc ttatgtactg gaatttgttg acgaaccgac
    12481 gccggaagac atcgtgtttg aagacaaagg cgtgaaagtc gtggtcgatg gcaaaagcct
    12541 gcaatttctg gacggtacgc agctggactt cgtaaaagaa ggcctgaacg aagggtttaa
    12601 attcaccaac ccgaacgtta aagatgagtg tggttgcggc gaaagcttcc acgtttgatg
    12661 cgcataccga taaccccacc gtggtcgcct gcgcgtgggg tttgttttac ctgattcgcc
    12721 gttatcgcgg cggatcgcag ccctgagaat gttatggatt acttcaccct ctttggcttg
    12781 cctgcccgtt atcaactcga tacccaggca ctgagcctgc gttttcagga tctacaacgt
    12841 cagtatcatc ctgataaatt cgccagcgga agccaggcgg aacaactcgc cgccgtacag
    12901 caatctgcaa ccattaacca ggcctggcaa acgctgcgtc atccgttaat gcgcgcggaa
    12961 tatttacttt ctttgcacgg ttttgatctc gcctgcgagc agcatactgt gcgcgacacc
    13021 gcgttcctga tggaacagct ggaactgcgc gaagaactgg acgagatcga acaggcgaaa
    13081 gatgaagcgc ggctggaaag ctttatcaaa cgtgtgaaaa agatgtttga tacccgccat
    13141 cagttgatgg ttgaacagtt agacaacgag acgtgggacg cggcggcgga taccgtgcgt
    13201 aagctgcgtt ttctcgataa actgcgaagc agtgccgaac aactcgaaga aaaactgctc
    13261 gatttttaat ttctggaagc taaacatggc cttattacaa attagtgaac ctggtttgag
    13321 tgctgcgccg catcagcgtc gtctggcggc cggtatcgac ctgggcacaa ccaactcgct
    13381 ggtggcgaca gtgcgcagcg gtcaggccga aacgttagcc gaccatgaag gccgtcacct
    13441 gctgccatct gttgttcact atcaacagca agggcattcg gtgggttatg acgcgcgtac
    13501 taatgcagcg ctcgataccg ccaacaccat tagttctgtt aaacgtctga tggggcgctc
    13561 gctggctgat atccagcaac gctatccgca tctgccttat caattccagg ccagcgaaaa
    13621 cggcctgccg atgattgaaa cggcggcggg gctgctgaac ccagtgcgcg tttctgcgga
    13681 catcctcaaa gcactggcgg cgcgggcaac tgaagccctg gcaggcgagc tggatggtgt
    13741 cgttatcacc gttccggcgt actttgacga tgcccagcgt cagggcacca aagacgcggc
    13801 gcgtctggcg gggttgcatg ttctgcgctt acttaacgaa ccgaccgctg cggctattgc
    13861 ctacgggctg gattccggtc aggaaggcgt gatcgctgtt tatgacctcg gcggcggtac
    13921 gtttgatatt tccattctgc gcttaagtcg cggcgtgttt gaagtgctgg caaccggcgg
    13981 tgattccgcg ctcggcggcg atgatttcga ccatctgctg gcggattaca ttcgcgagca
    14041 ggcgggcatt cctgatcgta gcgataaccg cgttcagcgt gaactgctgg atgccgccat
    14101 tgcagccaaa atcgcgctga gtgatgcgga ctccgtgacc gttaacgttg cgggctggca
    14161 gggcgaaatc agccgtgaac aattcaatga actgatcgcg ccactggtaa aacgaacctt
    14221 actggcttgt cgtcgcgcgc tgaaagatgc gggcgtagaa gctgatgaag tgctggaagt
    14281 ggtgatggtg ggcggttcta ctcgcgtgcc gctggtgcgt gaacgggtag gcgaattttt
    14341 cggtcgtcca ccgttgactt ccatcgaccc ggataaagtc gtcgctattg gcgcggcgat
    14401 tcaggcggat attttggtgg gtaacaagcc ggacagcgaa atgctgctgc ttgatgtgat
    14461 cccgctgtcg ctgggcctcg agaccatggg cggcctggtg gagaaagtga tcccgcgtaa
    14521 taccacgatt ccggtagccc gcgcccagga tttcaccacc tttaaagatg gtcagacggc
    14581 gatgtctatc catgtaatgc agggtgagcg cgaactggtg caggactgcc gctcgctggc
    14641 gcgttttgcg ctgcgaggta ttccggcgct accggctggc ggcgcgcata ttcgcgtgac
    14701 gttccaggtc gatgccgacg gtcttttgag cgtgacggcg atggagaaat ccaccggcgt
    14761 tgaggcgtct atccaggtca aaccgtctta cggtctgacc gatagcgaaa tcgcttcgat
    14821 gatcaaagac tcaatgagct atgccgagca ggacgtaaaa gcccgtatgc tggcagaaca
    14881 aaaagtagaa gcggcgcgtg tgctggaaag tctgcacggc gcgctggctg ctgatgccgc
    14941 gctgttaagc gccgcagaac gtcaggatat cgacgatgct gccgctcacc tgagtgaagt
    15001 ggcgcagggc gatgatgttg acgccatcga acaagcgatt aaaaacgtag acaaacaaac
    15061 ccaggatttc gccgctcgcc gcatggacca gtcggttcgt cgtgcgctga aaggccattc
    15121 cgtggacgag gtttaatatg ccaaagattg ttattttgcc tcatcaggat ctctgtcctg
    15181 atggcgctgt tctggaagct aatagcggtg aaaccattct cgacgcagct ctgcgtaacg
    15241 gtatcgagat tgaacacgcc tgtgaaaaat cctgtgcttg caccacctgc cactgcatcg
    15301 ttcgcgaagg ttttgactcg ctgccggaaa gctcagagca ggaagacgac atgctggaca
    15361 aagcctgggg gctggagccg gaaagccgtt taagctgcca ggcgcgcgtt accgacgaag
    15421 atttagtggt cgaaatcccg cgttacacta tcaaccatgc gcgtgagcat taacagaggt
    15481 tagtatggga cttaagtgga ccgatagccg cgaaattggc gaagcactgt acgatgcgta
    15541 tcccgatatt gatccgaaaa tggttcgatt caccgatatg catcagtgga tttgcgctct
    15601 ggaagatttc gacgacgacc cgcaggcatc gaatgagaaa atcctcgaag cgattttgtt
    15661 ggtctggctg gacgaagccg agtgaatgaa cgggctgcct gagggtagcc cgttttgctg
    15721 ttaaacgaat aaggataact aaaatgacag aagcgatgaa aattaccctc tctacccaac
    15781 ctgccgatgc gcgctgggga gaaaaagcaa cttacagcat taataatgat ggcattaccc
    15841 tgcacttgaa cggagcggac gatctggggc tgatccagcg tgcggcgcgc aagattgacg
    15901 gtctgggcat caaacatgtt cagctaagcg gtgaaggctg ggatgcggat cgttgctggg
    15961 cattctggca aggttacaaa gccccgaaag gcacgcgtaa agtggagtgg ccggatctgg
    16021 acgatgtcca gcgccaggaa ctggataacc gcctgatgat catcgactgg gtgcgtgaca
    16081 ccattaacgc accggcggaa gagctggggc catcgcaact ggcacagcgt gctgttgatc
    16141 tgatcagcaa tgtcgcgggc gatcgtgtga cttaccggat caccaaaggc gaagatctgc
    16201 gtgatcaagg ttatatgggg ctgcacacgg tcggacgcgg ttcagaacgt tctccggtat
    16261 tgctggcgct ggattacaac ccgactggcg ataaagaagc gccagtgtac gcgtgcctgg
    16321 taggtaaagg tatcactttt gactccggcg gctacagcat caaacagacc gcatttatgg
    16381 actcgatgaa gtcggacatg ggcggcgcgg caacggttac cggggcgctg gcgtttgcca
    16441 ttacgcgcgg actgaacaag cgcgtgaagc tgttcctctg ctgtgcggat aacctgatca
    16501 gcggcaacgc gttcaaactg ggcgatatta ttacctatcg caacggtaaa aaagttgaag
    16561 tgatgaacac tgatgcggaa gggcgtctgg tgctggccga tggcctgatt gatgccagtg
    16621 cgcagaaacc ggaactgatc attgatgcgg cgaccctcac cggggcggcg aaaactgcgc
    16681 tgggtaatga ttatcacgcg ctgttcagtt ttgacgatgc gcttgccggt cgcttgctgg
    16741 cgagtgccgc gcaggagaat gaaccgttct ggcgtctgcc gctggcggag ttccaccgca
    16801 gccagctgcc gtctaacttt gccgaactga acaataccgg aagcgcggcg tacccggcag
    16861 gcgcgagcac ggcggcgggc ttcctgtcgc actttgttga gaactatcag caaggctggc
    16921 tgcatatcga ctgctcggcg acttaccgta aagcgccggt tgaacagtgg tctgcgggtg
    16981 ctacgggact tggtgtgcgc acgattgcta atctgttaac ggcgtaaact aatatgccgg
    17041 atgcggcgta aacgccttat ccggactaca aattgcgaaa atccaataga ttgcgactcc
    17101 cttgtaggcc tgataagcgt ggcgcatcag gcacttgtgc cccgaacgtt gaatgaattg
    17161 tggttcttat gtccgaaaca aaaaatgaac ttgaagacct gctggaaaaa gcagcaaccg
    17221 aaccggcgca ccgcccggcc tttttccgta ctctactgga atccaccgtc tgggtgccgg
    17281 gtacggcggc gcagggcgag gctgtggttg aagatagcgc gcttgattta cagcactggg
    17341 aaaaagaaga cggcaccagc gtcattcctt tttttacctc gttagaagca cttcaacagg
    17401 cggttgaaga cgaacaggca tttgtcgtaa tgcccgttcg cacattgttt gagatgaccc
    17461 ttggcgaaac gctcttcctt aatgccaaac tgccaaccgg taaagaattt atgccgcgtg
    17521 aaatcagttt gttgattggt gaagaaggaa atccgctgag cagccaggaa gtactggagg
    17581 gcggtgaatc gctgatatta tcggaagtcg cagagccacc agcacaaatg attgattctc
    17641 tcaccacctt atttaaaact attaagccgg tgaagcgtgc ttttatttgt tcaattaaag
    17701 agaacgaaga ggcacagcct aatttactta ttggcattga agccgatggt gatatcgaag
    17761 aaattattca ggcggcggga agtgtagcga ccgatacaat acctggcgat gaacccatcg
    17821 atatttgtca ggtgaaaaaa ggggaaaaag gaattagcca ctttattacc gaacatattg
    17881 tgccattcta tgaacgtcgc tggggtggtt ttttgcgtga ctttaaacag aaccggataa
    17941 tctaaaatat gccgccccaa agggcggcat tcgggcttac ctgctgttct gaaggttgtt
    18001 aaccaccaat aaattgttac cggagagact ccggttatta tttacctcgg ttaacagcgc
    18061 accagaaaca ataagtacga tacaaaagca ccgtatcttc attgtgcggt ccatgcgaag
    18121 accgcatcac gacagcacca ctggcgatat tgccgcgata gcaagcgctg tgaaacacct
    18181 gcatttacgg ccatggaaca tcctttcctt aagccagcgc ccgtttgcga gacggcggaa
    18241 agggccggtg tcgataatac gctaaataaa caatgattac tctctttcgc ttgacaaaaa
    18301 gagagttact ggcgagtatt gctttgctgt gaagcacctg cgttgctcat gcaacacaag
    18361 ccaatctgct ggaaaggccg cgaaccagac cagcaataaa aaaccgccaa attcggcggt
    18421 tttttattgc ctgttaaaag cttatttctc tggctcaacc ggtaaatctg ctcgtgcgcc
    18481 ccattcactc catgcgccgt cgtacagttt cacgtttggc acatcaagtg tcgcgagtgc
    18541 caacaaaacc acggctgccg ttacaccaga gccgcagctg acgataatcg gtttgtcata
    18601 gctgacgccg cgaccaaaaa atatcgcatc cagttcatcg gtcgttttta gttcgccttc
    18661 gcgcaccagt tccgtccacg gaacgttcaa tgcaccggga atatgtccgc gacgtaaacc
    18721 tgggcgaggt tcatcaactt ctgcgttaaa acgtgcagcc gggcgggcat caataatttg
    18781 cgccgtattt tcatggcttg ccaataacac atcggtgact ttcaccacgg cttcaggatt
    18841 aaacgtggcg ttaaactctc cttccggcag ctctactgca ccttcttcta acagcagatc
    18901 atcgcgctgc cagcctgcaa gtccaccccc cagaatcgac actttctcta caccaaaggt
    18961 gcgcagcatc caccatgctc gtggggctga gaaaagatta ccttcgtcat agataatcag
    19021 gtgcttatcc tggttaacgc ctaattcacg catcgccacg gcgaacgttt ccgggcgcgg
    19081 cagcatgtgc ggaagcgggg aagtgtgatc agaaagcgct tcgatatcaa aaaacactgc
    19141 gccgggaata tgtccattca gatactcctg agcaacgtta cgatcttcct gtccaggcga
    19201 cgccatgcgg gcatcgataa tctgaatttc cgggtcatca atatgttcgg cgagccagtc
    19261 ggctcctaca aaccatgtcg tggacatagg catctccatt tttgccgggg ttaggctaaa
    19321 ttgtcggatg tttagtgaaa agcgacaagc aaaagaggct gatttggctg tctgagccac
    19381 aattctcact aaaattcgga ttgtatgggc gtgatactga attacataac gtaaattctc
    19441 acgcataata attattcgac gttgctaacg ggccattgtg gcagggatga aaaatgaaaa
    19501 agttacgctt agccgcctgc atgctaatgc tggcgctggc agggtgcgac aacaacgata
    19561 acgcgccaac agcggtgaaa aaagatgcac cttctgaagt tactaaagcg gcatcttcag
    19621 aaaacgcgag ttcagcaaaa ctctctgcac cggagcgaca gaaactggcc caacagagtg
    19681 ccggtaaggc gctgacattg ctggatctct ctgaagtcca acttgatggc gcagccacgc
    19741 tggtgctgac cttctctatc cctctcgacc cggatcagga tttctcgcgc gttattcacg
    19801 tcgttgataa aaaaagcggc aaagtggatg gggcctggga gctgtcagat aatcttaaag
    19861 agctgcgttt acgccacctc gagccgaaac gtgatttgat cgttactatt ggcaaggagg
    19921 tcaaggcact caacaacgca accttcagta aagattacga aaaaactata actacccgcg
    19981 atatccagcc cagcgtcggt tttgccagcc gtggttcgct gctgcctgga aaagtcattg
    20041 aagggctgcc ggtaatggcg ctcaatgtta acaatgtcga tgttaacttc ttccgtgtta
    20101 agccagaatc tctgccagca tttattagcc aatgggaata ccgcaattcg ctggcgaact
    20161 ggcagtcaga taaactgctg cagatggcgg atctggtcta caccggacgg tttgatctca
    20221 atcctgcgcg taacacccgt gaaaaattat tgctgccgct gggcgatatc aaaccgcttc
    20281 agcaggcggg cgtgtatctg gctgtgatga atcaggctgg acgttacgat tacagtaatc
    20341 ccgcgacgct gtttacgtta agtgatattg gcgtttcagc tcaccgttat cacaatcgtc
    20401 tggatatctt tacccaaagt ctggaaaacg gcgcggccca gcaaggaatt gaagtctctt
    20461 tattaaatga gaaagggcag actctgactc aggcaaccag tgacgctcag gggcatgtgc
    20521 agctggaaaa tgataaaaac gcggcattac tgctggcgcg taaaaacggt cagacaacgc
    20581 tgctcgattt aaaactcccg gcgctggact tagcggaatt taacattgct ggcgcgccgg
    20641 gctatagcaa acagtttttc atgtttggcc cgcgcgatct ttatcgcccg ggtgaaacgg
    20701 tcatcctcaa tggtttgctg cgtgatgcag acggtaaagc attgcccgat caacccatca
    20761 agttagacgt gattaaaccc gacgggcagg tactcaggag cgtcgttagt cagccggaga
    20821 atggcctcta ccactttacc tggccactcg atagcaatgc ggcaaccggt atgtggcata
    20881 ttcgcgctaa cacgggcgat aaccagtatc ggatgtggga tttccacgtc gaagatttta
    20941 tgccagagcg tatggcgctg aatctgaccg gtgagaaaac cccgctaacg ccgaaagatg
    21001 aagtgaaatt ctccgtggtg gggtactacc tgtatggtgc acctgctaat ggtaatactt
    21061 tgcaagggca acttttcctg cgcccactgc gtgaagctgt gtcagcctta cctggttttg
    21121 aattcggcga tatagctgcc gaaaatcttt cccgcacgct ggatgaagtt cagttgacgc
    21181 tggatgataa agggcgtggc gaagtttcca ctgaaagcca gtggaaggaa acgcattccc
    21241 cattacaggt tattttccag gggagtttgt tggaatcggg cggtcgcccg gtgacgcgcc
    21301 gcgctgagca ggctatctgg cctgccgatg cattgccggg gatccgtccg cagttcgcct
    21361 cgaaatcggt ttacgattat cgcactgaca gcacggtgaa acagcccatt gttgatgaag
    21421 gcagtaacgc cgcttttgac atcgtttata gcgatgcgca aggcgtaaaa aaagccgtgt
    21481 cgggcttgca ggtgcgcctg attcgcgaac gccgcgatta ctactggaac tggtcagaag
    21541 atgaaggctg gcagtcacag tttgatcaaa aagatctgat tgaaaatgaa caaactctgg
    21601 atctgaaagc ggacgaaacc ggcaaggtca gtttcccggt agagtggggc gcttatcgtc
    21661 tggaagtcaa agcgccgaat gaagcggtca gtagtgtgcg tttctgggct ggctatagct
    21721 ggcaggacaa cagcgacggg agcggtgccg tgcgacccga ccgtgtcacg ctgaaactgg
    21781 ataaagccag ttatcgccct ggcgatacca ttaagttgca tattgccgcg ccaacggcgg
    21841 gtaaaggtta tgcgatggtc gagtccagtg aagggccgct gtggtggcaa gagattgatg
    21901 ttccggctca agggctggat ctgacgattc cggtaaataa aacctggaat cgtcatgatc
    21961 tttatttgag tacgctggtg gtgcgtcctg gcgataaatc tcgctccgcg acgccaaaac
    22021 gcgcggttgg ggtgttgcat ctgccgcttg gtgatgaaaa ccgtcgcctc gatctggcgc
    22081 tggaaacccc caccaaaatg cgccccaatc agccattaac cgtgaaaatt aaagccagca
    22141 ctaaaaatgg tgagatgcca aaacaggtga atgtgctggt gtctgccgtt gatagcggtg
    22201 tgctgaatat tactgattac gtcacgccag atccgtggca ggcgttcttt ggtcagaaac
    22261 gctatggcgc agacatttac gatatttacg gtcaggttat tgaaggtcag gggcgtctgg
    22321 cagctctgcg tttcggtggc gatggtgatg agctgaaacg tggtggtaaa ccgccggtca
    22381 atcacgtcaa tattgtcgcg cagcaggcgc tgccggtaac gctcaacgaa cagggcgaag
    22441 gctcggttac actgccaatt ggcgatttta atggtgagtt acgagtcatg gcgcaggcct
    22501 ggacagcaga tgatttcggt agcaacgaaa gcaaagtggt tgttgccgca ccggtgatag
    22561 cagaactgaa catgccgcgc tttatggcca gcggtgacac ctcacgtctg acgctggata
    22621 tcactaatct taccgataaa ccgcaaaaac tgaacgttgc cctgaccgcc agtggtttgc
    22681 ttgaactggt cagcgattca cccgcagccg ttgaattagc gccaggtgtg cgtactacgc
    22741 tgtttatccc ggtgcgagca ttgccgggtt atggtgatgg tgaaattcag gccaccatta
    22801 gcgggttagc gttacccggg gaaaccgttg ccgatcagca taaacagtgg aaaatcggcg
    22861 tccgtccggc gttcccggca caaacggtca attacggtac ggcgttgcag cctggtgaga
    22921 catgggcgat tccggcggat ggattgcaaa acttctcgcc tgttacgctg gaagggcaat
    22981 tgttgttgag cggcaaacca ccgctgaaca tcgcaagtta tatcaaagag ttaaaagcgt
    23041 atccgtacgg ctgtcttgag caaaccgcca gcggcctgtt cccgtcactt tataccaacg
    23101 cagcccaact gcaggcgttg ggcatcaaag gcgacagtga tgagaaacgc cgtgcatcgg
    23161 tcgatatcgg catttcccgt ttgctgcaaa tgcaacgtga taacggcggc tttgcgctgt
    23221 gggataaaaa cggtgacgaa gagtactggc tgacggctta cgtgatggat ttcctggtcc
    23281 gcgcaggtga gcagggttac agcgtgccga cagacgccat taaccgggga aatgagcgtc
    23341 tgctgcgcta tttacaagat ccgggcatga tgtcgatccc gtacgcggat aatctcaaag
    23401 ccagtaaatt cgccgtacag tcttacgctg cgctggtgct ggcccgtcag caaaaagctc
    23461 cactgggtgc gctgcgtgaa atctgggagc atcgtgcaga tgccgcttct ggtttaccgc
    23521 tgctgcaact tggcgttgcg ctgaaaacca tgggtgatgc gacgcgtggt gaagaagcaa
    23581 ttgcgctggc gctgaaaacg ccgcgtaatg atgagcggaa atggctgggt gattacggta
    23641 gcccactgcg cgacaacgcg ttaatgctct ccttgctgga agaaaataaa ctgctacccg
    23701 atgagcagaa cactttgctg aatacgcttt cgcagcaggc gtttggtgaa cgctggctat
    23761 cgacgcagga aagtaacgcg ttgttcctgg ctgcccgtac ggttcaggat ttaccgggta
    23821 aatggcaggc gcaaacctct ttctcagctg agccgctgac aggcgagaaa gcgcaaacca
    23881 gcaatctgaa tagcgatcaa cttgccacct tgcaggtgac caacagtggc gatcagccgt
    23941 tatggctgcg tgtggatgcc agcggttatc cgcaatccgc acctttaccg gcgaacaatg
    24001 tgctgcaaat cgagcgtcat attcttggta ctgatggtaa gagcaaatcg ctggactcgt
    24061 tacgtagcgg cgatctggtg ctggtgtggt tgcaggttaa agccagtaac agcgtgccgg
    24121 atgcgttggt cgtggatctg ctgccagcgg gtctggaact ggaaaaccag aatctggcga
    24181 acggtagcgc cagcctggag caaagtggtg gcgaagtgca gaacttactg aaccagatgc
    24241 agcaggcgag tattaagcac attgagttcc gtgacgatcg ctttgtggcg gcggttgccg
    24301 ttgatgaata ccaaccggta acgctggtgt atctggcgcg ggcggtgacg ccgggaacgt
    24361 atcaggtacc gcaaccgatg gtggaatcaa tgtatgttcc ccaatggcgg gcgaccggcg
    24421 cggctgaaga tttgctaatt gtcagaccgt aaatgcctcg cttgttaacc aaacgcggct
    24481 gctggataac gctggcagct gcgccatttc tcctttttct tgcagcgtgg ggagcagata
    24541 aactctggcc tctaccgctg catgaagtca atcccgcacg agtggttgtg gcgcaggatg
    24601 ggacgccgct ctggcgcttt gccgatgcgg aaggcatctg gcgttatccg gtaacaatcg
    24661 aagatgtttc tccacgttac cttgaagcgc tgatcaatta tgaagatcgc tggttctgga
    24721 agcatccggg ggtgaatcca ttctcggtgg cgcgcgcagc atggcaagat ctcacttcgg
    24781 gaagggttat ttccggtggc agcacgctca ctatgcaggt tgctcgtctg cttgatcctc
    24841 accccaaaac atttggcggc aaaattcgcc agctctggcg cgcgttgcaa ctggagtggc
    24901 atctgtctaa gcgtgaaatt ctgaccttgt atcttaaccg cgctccgttt ggcggtacgt
    24961 tgcaggggat cggtgcggca agttgggctt atctcggaaa atcgcctgcg aatttaagct
    25021 attccgaggc ggcaatgctg gcggtgttgc cgcaagcgcc cagccgactg cgcccggatc
    25081 gctggccgga acgtgccgaa gccgcgcgta ataaagtgct cgaaaggatg gccgtgcaag
    25141 gtgtgtggtc ccgtgagcag gtaaaagagt caagggaaga acccatctgg ctggcacccc
    25201 gacaaatgcc gcaactggca ccgctgtttt cgcgcatgat gctcggtaaa agcaaaagcg
    25261 acaaaatcgt tacgacgctg gatgccggtc ttcaacgacg tctggaagaa ctggcgcaaa
    25321 actggaaagg acgattacca ccgcgcagct cactggcgat gatcgtggtt gatcataccg
    25381 atatgcgtgt tcgcggctgg gtgggttcgg ttgatctcaa cgatgattca cgctttggtc
    25441 atgttgatat ggtcaatgcg atccgatcgc caggatcggt gctcaaaccg tttgtttatg
    25501 gtctggcgct ggatgaaggc ttgatccacc cggcatcgct gttgcaagac gtcccccgac
    25561 gcaccggtga ttaccgacct ggcaactttg acagcggttt tcatggcccg atcagcatga
    25621 gtgaggcgct ggtgcgatcg ctgaacttac ctgctgtgca ggtgctggaa gcctatggac
    25681 cgaaacggtt tgcggcaaag ttacgcaatg ttggattgcc gttatatttg cccaacggtg
    25741 ctgcaccgaa tctttcgctc attctcggcg gcgctggcgc aaaactggaa gatatggcgg
    25801 cagcgtatac cgcgtttgct cgccacggca aggcaggcaa attgcgctta cagcctgacg
    25861 atccgctgct tgaacggcct ttaatgtcgt cgggggctgc gtggatcatt cgccggatta
    25921 tggctgatga agcgcaaccc ttgccggatg gtgccttgcc gcgcgtcgcc ccactggcat
    25981 ggaaaacggg caccagctat ggctatcgtg atgcctgggc gattggggtt aacgctcgct
    26041 atgtcattgg gatctggact ggcagaccgg acggcacgcc cgttgttggt cagtttggct
    26101 ttgccagcgc cgtaccattg ttgaatcagg tcaataatat cttactgtcg cgcagtgcga
    26161 atctgccgga agacccacgt cctgactctg taagtcgtgg cgttatctgc tggccgggcg
    26221 gacagtcttt gccggaaggt gacagtaact gtcgccgccg cctggcaacc tggctgctgg
    26281 acgggagtca gccgccaact ctattactgc cggagcagga aggcattaat ggcattcgtt
    26341 tccccatctg gctggatgaa aacggcaaac gtgttgccgc tgattgccct caggcgcgac
    26401 aagaaatgat aaatgtctgg ccgctaccgc tggaaccctg gctgcccgca tcagaacgtc
    26461 gcgctgtgcg cttgccaccg gcctcgacaa tttgcccgcc atacggtcac gatgcacaac
    26521 tcccgctgca gctaacaggt gtgcgcgatg gcgcgattat taaacgttta ccgggcgcag
    26581 cggaagcgac tttgccgtta caatcaagtg gaggggcagg tgaacgctgg tggtttctga
    26641 acggcgaacc gttaactgaa cgtgggcgca acgtgaccct gcatttgacg gataaaggcg
    26701 attatcaatt gctggtgatg gatgaggtgg ggcaaatagc gacagtgaaa tttgtcatgc
    26761 aatagtctgt tttgtccctg attgttgctt aaactgggtt tattttaaaa aaatgttacc
    26821 ttcatcaata gtcaacggct ctgttgctca ttataatccg cgccatctcg tacgctggta
    26881 cagacaacaa cagaacaatt tacagaggta aaaatggcta ttgaacgtac tttttccatc
    26941 atcaaaccga acgcggtagc aaaaaacgtg attggtagta tctttgcgcg ctttgaagct
    27001 gcagggttca aaattgtcgg caccaaaatg ctgcacctga ccgttgaaca ggctcgtggc
    27061 ttttatgctg aacacgatgg aaaaccgttc tttgatggtc tggttgaatt catgacttct
    27121 ggcccgatcg ttgtttccgt gctggaaggt gaaaatgctg ttcagcgtca ccgcgatctg
    27181 ctgggcgcga ccaatccggc aaacgcactg gccggtactc tgcgtgctga ttacgctgac
    27241 agcctgaccg aaaatggtac ccacggttct gattccgtcg aatctgccgc tcgcgaaatc
    27301 gcttatttct tcggcgaagg cgaagtgtgc ccgcgcaccc gttaataatt tcgtaaatgc
    27361 cgcgtgcaaa cgtggcatcc gtgcgccaga atttgtacaa tgcagcgccc ccggacgagc
    27421 agccgctcac cggggcgttt cttttttcaa ccctccaggg gccataacgt gtaataacga
    27481 ggccggaata gcatatgtct gaacaattag tcacacctga aaacgtcacc actaaagatg
    27541 gaaaaatcaa cctgctggat ctcaaccgtc agcagatgcg ggagtttttt aaagatttag
    27601 gtgaaaaacc cttccgcgcc gatcaggtga tgaagtggat gtatcactat tgctgcgaca
    27661 actttgatga gatgaccgac atcaataaag tgttgcgcgg caaactgaaa gaggtggcgg
    27721 aaatccgtgc accggaagtg gttgaagaac agcgttcatc tgacggcacc attaaatggg
    27781 cgatcgccgt tggcgatcaa cgcgtcgaaa cggtgtatat cccggaagac gaccgcgcaa
    27841 cgctctgcgt ctcttcgcag gtggggtgtg cgctggagtg taaattctgt tccaccgccc
    27901 agcagggctt taaccgcaac ctgcgggtat cggaaattat cggccaggtg tggcgtgcgg
    27961 cgaaaatcgt cggcgcagca aaagtcaccg gtcagcgccc aatcactaac gtcgtgatga
    28021 tgggtatggg cgagccgctg ctcaacctga acaatgtcgt tccggcgatg gagatcatgt
    28081 tggatgactt tggttttggt ctgtctaaac gtcgcgtcac gctttccact tccggtgtag
    28141 ttccggcgct ggataaactg ggcgatatga tcgacgttgc actggcaatt tccctgcacg
    28201 cgccgaacga cgaaattcgt gacgaaattg tgccgatcaa caaaaagtac aatatcgaaa
    28261 cgttccttgc ggcggtgcgc cgttatctgg agaaatccaa cgccaatcag ggccgagtca
    28321 ctattgaata cgtgatgctt gaccacgtta acgacggcac tgaacacgcg caccaactgg
    28381 cggagctact gaaagatacg ccgtgtaaga tcaacctgat cccgtggaac ccgttccctg
    28441 gcgcgccgta tggacgcagt tcgaacagcc gtatcgaccg tttctcaaaa gtgctgatga
    28501 gctacggttt caccactatt gtgcgtaaaa ctcgtggtga tgatatcgat gctgcctgtg
    28561 gtcagttggc gggcgatgtt atcgaccgta cgaaacgtac cctgcgtaaa cggatgcagg
    28621 gtgaagccat cgatattaaa gcggtctgat agccattacg ccacggttac tttatgtacc
    28681 gtggcgtaat cttttatgaa tgaagtatta agttgcaatt gtctggtcaa tattaacggt
    28741 gcgtttttgc tgactttaag gcagtatgta acgatgcaac agtaagttag ccttagtgaa
    28801 tgtgggcttt gtcacgagca cacagacggt cttatactgt atgataacgg ttaacttaac
    28861 ggatgtttcg cggtgtgggt gagcatgatg gttcaccggc atctcaattc tcatttaaac
    28921 gtacctgcag cgaatgaata ctgaagccac gcacgaccaa aatgaagcac ttactaccgg
    28981 cgctcgcctg cgtaatgctc gcgaacaact aggacttagt cagcaggccg ttgctgagcg
    29041 actttgcctg aaggtttcca cggtacgcga cattgaagaa gataaggcac ctgccgatct
    29101 tgcttcaaca ttcctgcgcg gatatatccg ctcttatgcg cgtctggtac atattccaga
    29161 agaagaactg ctaccggggc tggaaaaaca ggctccacta cgagctgcaa aagttgcgcc
    29221 gatgcagagt ttttccctcg gtaaacgccg caaaaaacgt gacggctggc tgatgacctt
    29281 cacctggctg gtgttgtttg tggttattgg cctgagcggt gcctggtggt ggcaagacca
    29341 caaagctcag caggaagaga tcaccactat ggccgatcaa tcttctgcgg aactgaataa
    29401 taatcaaagc cagagtgtac cgttagatac gtcgacaacc acagatcagg ctatggcgac
    29461 cacgccgaca tcacctgtgg ataccaccgc aactaacacg caaacacctg ccgcgacgac
    29521 ggctccatcg ccaaccgtcg attcacaaca gaatgcggtt gttccacctt cgcaggcaaa
    29581 tgttgatacc gctgcgactc cggcaccagc ggcaacaaca atgccagatg gtgctgcgcc
    29641 gttgccaacc gatcaggcag gcgtgactac gccggcggct gatccgaatg cgctggtgat
    29701 gaactttact gccgattgct ggctggaggt tactgatgct accggtaaaa aactgtttag
    29761 cggtatgcag cgtaaagacg gtaatttgaa cttaaccggc caggcaccgt acaaactgaa
    29821 aattggtgcg ccagccgcag tacagatcca gtatcaaggg aaacctgtcg atctgagtcg
    29881 ttttatcaga actaaccagg ttgcgcgtct gaccctcaat gccgaacaat caccggcgca
    29941 gtaacagacg ggtaacgcgg gagatttttc atgcataacc aggctccaat tcaacgtaga
    30001 aaatcaacac gtatttacgt tgggaatgtg ccgattggcg atggtgctcc catcgccgta
    30061 cagtccatga ccaatacgcg tacgacagac gtcgaagcaa cggtcaatca aatcaaggcg
    30121 ctggaacgtg ttggcgctga tatcgtccgt gtctccgtac cgacgatgga cgcggcagaa
    30181 gcgttcaaac tcatcaaaca gcaggttaac gtgccgctgg tggctgacat ccacttcgac
    30241 tatcgcattg cgctgaaagt agcggaatac ggcgtcgatt gtctgcgtat taaccctggc
    30301 aatatcggta atgaagagcg tattcgcatg gtggttgact gtgcgcgcga taaaaacatt
    30361 ccgatccgta ttggcgttaa cgccggatcg ctggaaaaag atctgcaaga aaagtatggc
    30421 gaaccgacgc cgcaggcgtt gctggaatct gccatgcgtc atgttgatca tctcgatcgc
    30481 ctgaacttcg aacagttcaa agtcagcgtg aaagcgtcag acgtcttcct cgctgttgag
    30541 tcttatcgtt tgctggcaaa acagatcgat cagccgctgc atctggggat caccgaagcg
    30601 ggtggtgcgc gcagcggggc ggttaaatcc gccattggtt taggtctgct gctgtctgaa
    30661 ggcatcggcg acacgctgcg cgtatcgctg gcggccgatc cggtcgaaga gatcaaagtc
    30721 ggtttcgata ttttgaaatc gctgcgtatc cgttcgcgcg ggatcaactt catcgcctgc
    30781 ccgacctgtt cgcgtcagga atttgatgtt atcggtacgg ttaacgcgct ggagcaacgc
    30841 ctggaagata tcatcactcc gatggacgtt tcgattatcg gctgcgtggt gaatggccca
    30901 ggtgaggcgc tggtttctac actcggcgtc accggcggca acaagaaaag cggcctctat
    30961 gaagatggcg tgcgcaaaga ccgtctggac aacaacgata tgatcgatca gctggaagcg
    31021 cgcattcgtg cgaaagccag tcagctggac gaagcgcgtc gaattgacgt tcagcaggtt
    31081 gaaaaataat aacgtgatgg gaagcgcctc gcttcccgtg tatgattgaa cccgcatggc
    31141 tcccgaaaca ttgagggaag cgttgagggt tcatttttat attcagaaag agaataaacg
    31201 tggcaaaaaa cattcaagcc attcgcggca tgaacgatta cctgcctggc gaaacggcca
    31261 tctggcagcg cattgaaggc acactgaaaa acgtgctcgg cagctacggt tacagtgaaa
    31321 tccgcttgcc gattgtagag cagaccccgc tattcaaacg tgcgattggt gaagtcaccg
    31381 acgtggttga aaaagagatg tacacctttg aggatcgcaa tggcgacagc ctgactctgc
    31441 gccctgaagg gacggcgggc tgtgtacgcg ccggcatcga gcatggtctt ctgtacaatc
    31501 aggaacagcg tctgtggtat atcgggccga tgttccgtca cgagcgtccg cagaaagggc
    31561 gttatcgtca gttccatcag ttgggctgcg aagttttcgg tctgcaaggt ccggatatcg
    31621 acgctgaatt gattatgctc accgcccgct ggtggcgcgc gctgggtatc tccgagcacg
    31681 taactcttga gctgaactct atcggttcgc tggaagcacg cgccaattac cgcgatgcgc
    31741 tggtggcatt ccttgagcag cataaagaaa agctggacga agactgcaaa cgccgcatgt
    31801 acactaaccc gctgcgcgtg ctggattcaa aaaatccgga agtgcaggcg cttctcaacg
    31861 acgctccggc attaggcgat tatctggacg aggaatctcg tgagcatttt gccggtctgt
    31921 gcaaactgct tgagagcgcg gggatcgctt acaccgtcaa ccagcgtctg gtgcgtggtc
    31981 tggattacta taaccgtacc gttttcgagt gggtgactaa cagcctcggt tcccagggca
    32041 ccgtgtgtgc aggcggtcgt tatgacggtc tggtggaaca actgggcggt cgtgcaacac
    32101 cggctgtcgg ttttgcgatg ggcctcgaac gtcttgtatt gttagtacag gccgttaatc
    32161 cggaatttaa agccgatcct gttgtcgata tatacctggt ggcttcaggt gctgatacac
    32221 aatctgcggc tatggcatta gctgagcgtc tgcgtgatga attaccgggc gtgaaattga
    32281 tgaccaacca cggcggcggc aactttaaga aacagtttgc ccgtgctgat aaatggggtg
    32341 cccgcgttgc tgtggtgctg ggtgagtctg aagtggctaa cggcacagca gtagtgaagg
    32401 atttgcgctc tggtgagcaa acggcagttg cgcaggatag cgtagccgcg catttgcgca
    32461 cgttactggg ttaaggaagg agaaggacag cgtggaaatt tacgagaacg aaaacgacca
    32521 ggttgaagcg gttaaacgct tttttgctga aaatggcaaa gcactggctg ttggggtgat
    32581 tttgggcgtt ggcgcactga ttggctggcg ctactggaac agccatcagg ttgattctgc
    32641 acgctccgct tctcttgcct atcaaaatgc ggttaccgca gtgagcgaag gcaaaccgga
    32701 tagcatcccg gcggcggaaa aatttgctgc tgaaaataaa aatacttatg gtgcgctggc
    32761 ttctttggaa cttgcgcagc aatttgttga caaaaatgaa ctagagaaag ctgccgccca
    32821 gttacaacag gggctggcag acacgagcga tgaaaatctc aaagccgtga taaatctgcg
    32881 tcttgctcgc gttcaggtac agctcaagca ggctgatgcc gcgctgaaaa cccttgatac
    32941 catcaaaggt gaagggtggg ctgccattgt tgccgacctg cgtggtgaag cattgctgag
    33001 caaaggtgat aagcaaggtg cgcgtagtgc atgggaagca ggcgtgaaaa gcgatgttac
    33061 cccggcactg agcgaaatga tgcagatgaa aattaataat ttgtccatct gagagggacc
    33121 cgatgcaatt gcgtaaatta ctgctgccag gactgctttc cgttaccctt ttaagcggct
    33181 gttcgctgtt taacagcgaa gaagatgtgg taaagatgtc cccattgcca accgttgaaa
    33241 accagtttac gccgtctact gcgtggagca cttccgttgg tagcggcatt ggcaacttct
    33301 attccaatct tcatccggca ctggcggaca acgttgtcta tgctgcggac cgcgctggtt
    33361 tagtaaaagc gctgaatgcg gatgacggca aagaaatctg gtctgtcaac ctggccgaga
    33421 aagatggctg gttctctaaa gatcctgcat tactttctgg cggcgtgacc gtgtctggtg
    33481 ggcatgtcta cattggcacc gaaaaggcgc aggtttacgc gctgaatacc agcgatggta
    33541 ctgtggcatg gcaaactaaa gtcgcgggtg aagcactttc gcgcccggtg gtcagcgacg
    33601 gtctggtgtt aatccacacc agtaacggtc agttacaagc gctgaacgaa gctgacggcg
    33661 ctgtcaaatg gacagttaac ctcgatatgc cttcgctctc tttgcgtggc gagtctgcgc
    33721 cggcaacggc ttttggtgcg gccgtcgtgg gtggcgataa tggtcgcgtc agcgcagtgc
    33781 tgatggaaca gggccagatg atttggcagc agcgtatttc ccaggcgacc ggttctaccg
    33841 aaattgaccg tctgagcgat gttgacacga cacccgtcgt tgttaacggc gttgttttcg
    33901 cgctggccta taatggtaac ctgacggcgc ttgatctgcg cagtggtcag attatgtgga
    33961 aacgcgaact gggttcggtg aatgatttca tcgtcgacgg caatcgcatc tatctggtcg
    34021 atcaaaatga ccgggtgatg gcgttgacca ttgatggcgg cgttacgctg tggacacaaa
    34081 gcgatctgct gcatcgcctg ctgacttctc cggtgctgta taatggcaac ctggtggtag
    34141 gtgacagtga aggttatctg cactggatta acgtcgaaga tggtcgtttc gttgcccagc
    34201 aaaaagttga tagttccggt ttccagactg aaccggttgc cgctgacggc aaactgctga
    34261 tccaggcaaa agacggaacc gtgtactcta ttacacgtta atcgtctctg tcgttcactt
    34321 tgaaaacggc tcctggacag gggccgtttt cctgttttta acaacgacac gaatatagcg
    34381 tgcgttgtct gatgatttat aaaaatgagg ctttaaacat ggtacctgtg gtcgcgcttg
    34441 tcgggcgccc taacgtagga aaatccacgt tatttaaccg tctaactcgc acccgagatg
    34501 cgctggttgc ggatttcccg ggtctgactc gtgaccgtaa gtacggtcgt gcggaaattg
    34561 aaggccgtga gtttatctgc attgataccg gcgggattga tggcacagaa gacggtgtag
    34621 aaacccgcat ggcggaacag tcgctgctgg cgattgaaga agcggacgtc gtactgttta
    34681 tggtggatgc gcgcgcgggc ctgatgccgg cggatgaagc gattgctaaa catctgcgct
    34741 cccgtgaaaa accgaccttc ctggtggcaa acaaaactga cggtctggat cccgatcagg
    34801 cagtggttga tttctactcg cttggtttag gtgaaatcta cccgatcgcc gcgtctcacg
    34861 gtcgtggcgt attaagtctg ctggagcacg tgctgctgcc gtggatggaa gatctcgcac
    34921 cgcaagaaga agtcgacgaa gacgccgaat actgggcgca atttgaagcg gaagagaacg
    34981 gcgaagaaga agaggaagac gacttcgacc cgcaaagtct gccgatcaaa ctggcgattg
    35041 tgggtcgtcc gaacgtcggt aagtctacac tcactaaccg tattcttggt gaagagcgcg
    35101 ttgttgttta cgacatgcct ggcacgacgc gtgacagtat ctacattcca atggaacgcg
    35161 acggacgtga gtatgtgctc attgacaccg ctggcgtacg taaacgcggc aaaatcaccg
    35221 atgcggtaga gaagttctcg gtaatcaaaa cgttgcaggc cattgaagat gccaacgtgg
    35281 tgatgttggt gattgatgcg cgtgaaggta tttccgatca ggatctctcc ttgctgggct
    35341 ttattctcaa tagtgggcgc tcacttgtga ttgtagtgaa caagtgggac ggcctgagcc
    35401 aggaagtgaa agagcaggtg aaagagacgc tggacttccg tctgggcttt atcgattttg
    35461 ctcgtgtgca ctttatctct gccttgcacg gcagtggtgt tggtaacttg tttgaatcag
    35521 tacgtgaagc gtatgacagc tccacccgtc gtgtggggac ctctatgctg acgcgcatca
    35581 tgacgatggc tgttgaagat caccaaccgc cgctggtacg cggtcgtcga gtgaagctga
    35641 aatatgccca cgccgggggt tataacccgc cgattgtggt gattcacggt aatcaggtga
    35701 aagacctgcc tgattcctac aagcgttacc tgatgaacta cttccgcaaa tcgctggacg
    35761 taatgggatc gccgattcgt attcagttca aagaagggga aaacccgtat gcgaacaagc
    35821 gtaataccct gacgccgact cagatgcgca agcgtaagcg tctgatgaag cacatcaaga
    35881 aaagtaagta atcacttatc tggtttccct ttccacacgg agagggaaac cgaataacac
    35941 gcgaggagta tttatggcac tcaactgtcc aacctgcaaa acccctctcg aacgcaatgg
    36001 cgggactgct cactgtgcaa cttgtgccaa agatttcacc attcaggctc tttgtcctga
    36061 ctgccaccag ccattgcagg tattaaaagc atgcggtgcg gtggattatt tctgccaata
    36121 tgggcacggt ttaatttcta aaacacgcgt tgaatttgtt attgtgtaat atcatttaaa
    36181 tatatgattt tatcattcat atttataata tggatgatta tgccattttt gtattagttg
    36241 aattactaat atgagtggaa aaaatcactt atttaaaaaa taatgatata tttatccttt
    36301 ctggcatgcc tttctgaata atcagggata caatattaaa gcttattttt gactttatca
    36361 ttgtcacagg aaaagtgcat gctgcgatgg acacgctgta ttatgctaac atttatctct
    36421 ggggctgctt ttgcggctcc agagataaat gttgagcata aagaatcatt acctgattta
    36481 ggtagtcagg ccgcacaaca agaagaacaa accaacaagg ggaaatcgct aaaagagcgt
    36541 ggtgccgatt acgtcattaa ctccgctacg caagggtttg aaaacctgac acccgaggcg
    36601 ctggagtccc aggccagaag ctatctgcaa agtcaaatta cctctacgac acaatcttat
    36661 attgaggata cactctcccc ctacggtaag gttcgaacga atctttctct tggccagggc
    36721 ggcgatctgg atggcagttc catcgactat tttgttccct ggtatgataa tcaaactact
    36781 gtttatttca gccaattttc tgcacaacga aaagaagatc gtacgatcgg gaatattggg
    36841 cttggtgtaa gacataattt tgataaatgg ttgttgggtg gaaatatatt ttatgattat
    36901 gattttaccc gtgggcatcg ccgtttaggt ttaggtgtcg aagcgtggac tgattattta
    36961 aaattctctg gcaactatta tcacccgctt tctgactgga aagactccga agatttcgat
    37021 ttttatgagg agcgtcctgc gcgcggctgg gatattcgcg ccgaagcctg gcttcccgct
    37081 tatccgcagc ttggtggtaa aattgttttc gaacagtatt acggtaatga agtcgcgctt
    37141 tttggcactg ataatctgga gaaagatccc ttcgctgtca cgctgggtgt gaaataccag
    37201 cccgttccgc tcattgctgt cggtacagat ttcaaagctg gcactggcga taataccgat
    37261 ctttctgtta acgcaacgct taattatcag tttggcgtcc cgctcaaaga ccagcttgat
    37321 cctgacaaag tgagcgctgc tcactcgctt atgggcagcc gtcatgactt tgttgaacgt
    37381 aacaacttta ttgttctgga atacaaagaa aaagatcccc tcgacgtcac tttgtggctg
    37441 aaagccgacg ccacgaatga acatcccgag tgcgtgatta aagatacgcc tgaagaagct
    37501 atcgggctgg aaaaatgtaa atggaccatt aacgcgctca ttaaccatca ctacaaaatc
    37561 gtcgctgcct cctggcaggc gaaaaacaat gctgcacgta cgctggtgat gccggttatc
    37621 aaagaaaaca cgcttaccga aggtaacaac aaccactgga atctggtgct gcctgcctgg
    37681 cagtacagtt ctgataaagc cgaacaagaa aaactcaata cctggcgggt tcgtctggcg
    37741 ctggaagatg aaaaaggtaa tcgacagaat tccggcgtgg tggaaatcac cgttcagcag
    37801 gatcgcaaaa ttgagctcat cgttaacaac atcgccgatg taccagatga gaacaatcac
    37861 agtcatgaag ccagcgcaca ggccgatggt gtagatggtg tggtgatgga tctcgatatt
    37921 actgatagct ttggcgacaa cactgaccgc aacggtaatg tcttgccgca ggataatctc
    37981 aacccgcagt tgtttgacgc caacgataaa aaagtgacgc tgacgaataa accctgcact
    38041 accgagactc cctgtgtctt tatcgccaaa caagataaag aaaaggggac cgttacgctc
    38101 tccagtacat tgcctggcac cttccgttgg aaggcgaaag ccgctcccta tgatgacagt
    38161 aactatgttg acgtaacgtt cctgggttct gacatcggcg ggctgaatgc ctttatctat
    38221 cgtgttggtg cggctaagcc cgtgaatctt atcggcaata aagagccgtt gcctctcaat
    38281 agcagctatc gctttgtgtt gtggcgtgac gctaacaaag acggcgtgtt ccagctatca
    38341 gagaaattca ctgaagagga gatgaaacag tatgactatc agtgggaatt cacgggacat
    38401 agcgtgaatg gcaataccgg tgcgcaagcc aataccacta acgccgatat agagatcccg
    38461 gcaacgaaca aggacgcggc aacgaagttt agtgcccagg taaccgatgg cgtgcaggga
    38521 tacggtttgc aggtcaatta cagcaagaaa taggatcagc gccatggctg atcgggccac
    38581 cgctgaatgt taaccaagga gagtgtttat gaaacctcat atgaaacgtg gcctgacaag
    38641 agtcgcgctg gcactgatgc tggctggata ctgtgccgtg ccagccgcta tggcggaaga
    38701 tgccgcctgg gtagccagtg ggacgaccgc tgaattcgaa gggaccatac cctggcttta
    38761 tcgcgagggt gggaatgcca cgattaactc ggatgatgcg gatcacatca aagtaacgtc
    38821 tgatggtaaa ggtactcgtc cttcaggtag cgagacggat aaacgccttt actcaggaga
    38881 taccatcacg ttggggtggg atatcggcga taccgaaggg gatatcgacg atggtccaga
    38941 cggtattgat gcgaaaacga cagccactat caaatggtac agttacagcg ataatgcggg
    39001 tggcggtaaa acggagctga ccgcagcggc aggcaagacg agttataaac tcactgatga
    39061 cgaacgtgag cgttatatcg gtgttgaaat ccagcccatc acccagaccg gtaatccttt
    39121 ccagggaaca tcgctgactc tgctggatat ttcaacggcc agcggtggcg gcagcgatac
    39181 ggataatgtt gatcctggcc cggttgtgaa ccagaacctg aaagttgcca tcttcgagaa
    39241 aggcaccagt accaacctta tcggtggtaa tacagccatt gcgcttaaca aaacgtatgt
    39301 ggccaaactg tactcggatg aaaaccagaa tggtaagtac gatgcgggta cggatgtgga
    39361 cgttaccgct aactacgact ttgcgtgggt ctttaacggt aacagtaaac aacttgcggc
    39421 ggcgggcggt attgccaacg ccagcttcga taacaatgac attgttattc cgcaaactaa
    39481 cgaacaagca agaaccagcc ttaacggtag tgaccgtaat ggtaagaccg gccttgcaat
    39541 cccggcaaac ggcgacggcg ttcagggcta tacgctgtct atcatttaca aacaccacta
    39601 atgcgcacaa cagcattccc gtatgggaat gctgttttcc gcctgttttg cttagcgacg
    39661 cgttaagcaa aacgggttga cgttttgttt atcatgctgg agttttctac atgtcggtat
    39721 gtctgaaacc aggaaaaatc attgtgcttc tgggcatgtt ggcagccttt atgctgtctg
    39781 actttgcacg ggcaggagtg gaatggcaga catatcctgg ttctacaggc gagtttaacg
    39841 gtacagttcc tattgccgat agcgctagcg tacccgttta tcagggaagc gttcagcttg
    39901 atcctgctgc atctcatgat gtggcgtttt ctgctaaacc gaatgaattt agcgttgacg
    39961 atgacgctgc taacctgata gtcgctaatc ctcaggacag tgaaggcgac cagttttcca
    40021 caccacctgc gctgcgctgg gaaaaccaga cgccgccgac cgtcagtctg gtgtgggcgg
    40081 atgccgccac gccggatacg ccgctcaacc cgcagcccat cgccaaccgc agcttttgcg
    40141 cgcaaggtct ggccgggcgt tcgctggtgg cctggccgca gatagataca caacaaacca
    40201 ttccactgct ttacctgctg acctcgaccg gttatcccta tgaggggacg gtggcgctgg
    40261 ctgaacagaa agtgacgctg aacatcgcgc ccgcacaggg cgatttaatt tcggtgagcg
    40321 ccagtggtta taacgaaacg atcgcggcgg caaaaaccac cgttggtggc actatcacgc
    40381 tgaccgtcac caccaaagat tgtcagggca acgtcgcggg taatattccg tttattatca
    40441 aacgtaaaga tgcccaaaac cgtcagggcg cggtgaataa taccgcgccg gtggtgctgg
    40501 ggagtaccga gttaacgacg acggcaaccg agtatcgtgg cacatcggat gccaacggta
    40561 cggcaaccat cacggtgacc caacctaacg gccccggcgt caaaacgccg ctggtggcga
    40621 gtatttccgg cattgcgcaa accagcgaaa cggcggtgat ttttaccgta cttaccagcc
    40681 cggacgtggc gcaggccacg atgtggggcc atatggcgga aacggttgag gcacacggct
    40741 acacgttcag caggccaaaa ctggcggcgg aagtcagcaa tgaaaatgcc acggtcgtcg
    40801 atcacaacga aacctggtcc actttcacct ggagcggggc agacagccat tgcaccgtgc
    40861 tgccgggcat gcgccatttc ggcgcactgg cgacggtgat tccctccacg gtacagactg
    40921 tccttggctg gccgatgcag gatgattatt actggacatc gctggcgggc actaccgggc
    40981 agcaccatgc agcggatgtc tctaaccgtg ctgaagcgca aaagccggat agcaccacgt
    41041 ttcttgtaag ctgcgtggac aaacccgcgc ctgacgtcga accgaaaatc gtccttacgc
    41101 cagaaaacta tgatgatacg gcacaggcga tgaaagcgaa ggtgggtgag gatgccacga
    41161 tgcgtctgac gatcaccgac aataaaaaca acgatcaacc gctggcgtat tattacttct
    41221 cgctgcatct ggatgacggc gttaaccgta agaatcaaac cgactccgcc tgggaagccc
    41281 atccggtgca gattactggc ggcagtaatt ttcgccaggt cgacgcacat acctatgaag
    41341 gcatgaccga cgccaacggc caggcttcgc tcaccctgag ccagcccggc ggcgcggggg
    41401 tgaaaaccca tatcacggcg aaaatgcgca gcgattttaa cgccacggat gccaaagatg
    41461 tgatttttac cgtaatcacc agcccggaca gcgacaaagc gcggatgtgg ggccatatgc
    41521 gcggcattat tgagtccggc agtctgtata aacgcccgtt gctggcggat gaaaccgagc
    41581 atgaactggg aacagtgcgg gaaaacaacg aagactgggc gttgtacgat caaaacacca
    41641 gtatgcaggc ggaatgcggg gtgggtcata taccgcgtca gagctcactg gaaagcctgt
    41701 tttcagccca tcccggtaac gcgattggca ctgagtatgg ctggcctacc gcacaacaga
    41761 gctacctaag cgcggtggag caagaaacgc attcatcggt gaatctgggg aacgggagcg
    41821 tcgacagcta ttccggcttt aagcagaatt acctctcctg ttcgggtaat gagatggtag
    41881 cgaatgttga agtcagcacc gatcatgatg tgtctgttgg cacccaagcg caagccaaag
    41941 tgggtgacac catcgtaatg accgtgcgta cgattaacgc gctaaataat tccccggtac
    42001 cctttagcgc attcaccatt accaaaggta tgggatataa ccgagcggga caggtgagcg
    42061 ggtttgatga cccgagtagc ggctcaatca cgatggataa ctcgcaatat ggtacatcac
    42121 agccatcgat ggtttatgcg ggaacaaccg atgtccgggg cgtggcgacg gtcgagatta
    42181 agcaaccaca gggcgtcggg ctaaaaacgg tgctgagcgt aacaccggta aattcctacc
    42241 tgccgaatac cgttaactac agcgttattt ttactacccc aaccagcccg gacgtttccg
    42301 gcgcgcagat gtggggccat atggatgaaa cgattaccgt tgattcatca acctttaccc
    42361 ggcctaggct ggcggcggaa atcgccagcc cggacggaac cctgactgaa aataacgaaa
    42421 tctgggcgcg cgtgagccag gccaatacgt ccagtaccag caaaggcggc tgcggcacca
    42481 atatgctgcc gcgtcgctcc cagctaagcg cgttgtatag cgccaacagt ggtaatgcag
    42541 tgcaaacgac gcacggctgg ccaacgcagc gtcagcccta ctggagcagc tctccggcgg
    42601 atgtgacgcc gcactttttc acgatagcgc tgaacgatgg cgcacaagct atcggcggcg
    42661 atacgccggt ttacgtcagt tgcctgacga cggcgaacaa acctgccagc agcattacgc
    42721 tggaggtggt tgataaagcg caatggaatg ccgggaataa cgcggcaacg ctgaaaaaag
    42781 gcgaaacgct acaggttaag gtgacggtaa aagatgcaca gggcaacgcg ttagcggata
    42841 tgccatttac cctcagtcgc ggtgatggct atacccgtag cggcgaaaaa catattgccg
    42901 gtagcggcga tgcgctggtg gcgccggtgg tggtcaatgg cgggctggcc gatgaaacaa
    42961 cgttgaatga caccgcaacg gtgtataccg ccatgaccgg cagtgacggc agcaaaattc
    43021 ttaatattac ccgtccggat acccacggca ccaaaacggc gctgacggca acgctttact
    43081 ccgatgcgac gaaaaaagac agcctggaca cgatctttac ggtggttacc agcccggata
    43141 gcagccaggc gaaaatgtgg gggcatatgc cggaaacggt gacggctgag gacggtacgg
    43201 tctttaaacg cccgcggtta ctgaaggaac tgagcagtca gactgggcga acatcaacgc
    43261 ttgaagacaa cgaaaactgg gcgttgttta acattaatta tgcgagctct tccacgacgt
    43321 atagtggctg tggaaccaac tatattccga ctcaggccgg tctgacgtca ctgtttgcta
    43381 ataatgcggg caacaccatg aaaaccgtgc agggctggcc ggtggcaacg cgctatttgt
    43441 ccaacacgtc cgacaatggc agcatggagc agcgtaacta caaggcggtc gatctcagca
    43501 atggcacgag tgcggcagtg tcatcaacgg cgctgcaact gctgacctgt cagactacac
    43561 cggtaaccac ggtgagccaa atcctgctgg aagcggcaga tccggcgacg ctggatacca
    43621 cctataacgt ggtaaaagcg aaaaaaggcg aagaatccgt ggtgcgcgtc accaccaaag
    43681 atgcacaagg caacctggtg ggcaatacgg catttatcct gacgcgtgcc aacagcgtga
    43741 gtcgggctaa tgcatcagcg acgatgagcg tgggctcgtt gacggtgacg gatgcctggg
    43801 gcaatacgcg aaataatttc cagtcgacaa gtgaaaccat ttacggcgtg accggtgccg
    43861 acgggtcaac gacattgacc ctgaaacagg ataacagtac cgggttaaga accgatctca
    43921 cggcaaaact ggatacgtcc agcagcgtca aggcaacggt accggtggtc tttaccgtcg
    43981 tgaccagccc ggattcgcca aaagcgaatt tttggggaca tatggccgaa acggtggcgg
    44041 cctccgatgg ttcagtctat aaacgcccac tgttgctggc agagctggcc aacactggcg
    44101 ggcgacaaat cagttcagag aacggggaaa gctgggtacg attcacctgg aatcagtcga
    44161 cagatccttc tgtaagcggc tgcggcgttg cgtatatgcc aacgttggca ggcctgcaag
    44221 cgttgtatga cgctaatagt ggtaatgcaa tgagtacggt acagggatgg ccggtaaaag
    44281 cgacctatct caccaatacc ccaagtgaca cgcaaacggg gagccgttac tacaatgtgg
    44341 tgcaactgga ttctggcgca gcctcgcaaa tcacgacgaa cacgggtgta ctgcaaacct
    44401 gccgtacaac gccgttgacg gctgccagcc agattacgct ggaggccgcg gatcctgcgc
    44461 aatttgtcag cattgacagc acgcttagcg ctgtcaaagt gcagaaaggc gatagcgtgc
    44521 cgatccgcat ttcgactaaa gatgcgcagg gcaatttcgt cggcaatacg ccgtttgccc
    44581 tgaaacacgc gaactcgatc aatcgtcaaa atgtgtcgtc atcgcagaaa gtgtcggtca
    44641 cgacggaggt gggtacgacg gtggatacca gtgcaacaac caccctgtat ggcgtaaccg
    44701 ggccagacgg aaccaccacg atgacgctcc gacaggatgc gtccaccggg ttaagaaccg
    44761 acttttatgc cctgttgaac gataccggcg tgtcgtcgga caccttgccg gtgatcttta
    44821 ccgtcatcac cagcccggat acgccgctgg cggccaactg gggccatatg gcggaaacct
    44881 ttaccagtag cgaaggcgtc accttcaagc ggccttccct caaagctgaa ctctctggcg
    44941 gtacggccat aacggtcaat aatgaagtct ggtcgcgcct gacagcggcg gagaaagtgg
    45001 atgccagcaa ggccgggtgt gatgagacgt atcaaccgct ggtgaatgac atgcaggggt
    45061 tgtatgcgga ttaccctaac ggccagttag gatcggtgct cggcttgccg acgtctgccg
    45121 ggtattggtg ggcctacgac atgatgatgg tttctggcaa ctggactaat caggcatttt
    45181 cacctgttaa tggtcagttg atgcaggcat cgtccagcta tacggcgatc gtgatgtgtc
    45241 tggtagaacc acatacggaa gcagccacaa tagagcttac ttcaacggcg caggacgcag
    45301 caaaatcggc cagtaatggt ggacgaccga gcgccgtagc gaagaaaggc gagacgattc
    45361 cgttgacggt gaccgtgcgt gatagcgccg ggaacccgat gccatatgct gagtttaccc
    45421 tcacgcgtga aacgacgctg gatcgctcga aagcaaccgt taacacgagt gcggacgacc
    45481 ttaccgtaac ggcattggta cccgcgaata caaatagtgt gttggcagca agtggagcaa
    45541 aactgatcgg cacgacgggc agcgatggca aagcgacctt tgaggtgagt cagaatgcga
    45601 cgaccgggct ggcgacgccg ttaaccgtga cgctggcgcg tgatacgacg aaagccgcca
    45661 cgctggatgt tatctatacg gtgatcacca gcccggattc gccgagtgcg aaattctggg
    45721 ggcatatgcc ggacaccttt accagctcga cgggcgtcac cttcaaacga ccgctgctga
    45781 aagcagaaac cagctcaggc tcatcgatta gctctaacgg cgaagtttgg tcatatatga
    45841 gcaacgcgca aaatttaaca tcgacagatt gcccgctcga aaatcaaccg cgcagtaatg
    45901 agctgttgga tttgtatagt gatcacccga acggcacgct aatgaccgac ctggggttac
    45961 ccgtatatgc aggtaactgg tgggcctatg atatggtcat gctgagtgga acgtcgtgga
    46021 cttatcaact catcagcctg aaagacggaa ccattacgca gaaggggaca accagtgcgt
    46081 tgatgctttg cctggcacag ccgcatccgg ccggcgtcag cgtaacattg acgtcatccg
    46141 cacaggatga cgccagaacc accagcaatg gcggccgtcc cagcgcgtca gcgaaaaaag
    46201 gtaactcaat accgattgtg gtaacggtaa aagatcgtga tggcaacccg ttggcgggcg
    46261 aagcggtgac gctcaaacgt gattcagcgt tgagccgttc aggaacaatc gtggggacgc
    46321 cagcgaatga aataaagttg acggaactga cgcccgtgtc ggcgacattc ccgctggttt
    46381 caaatggtac acaatggctt ggtttcaccg gtagcgatgg tacggcgaca ttcaatgttg
    46441 aacagcctga tactgtcggc ctggcaatcc cctttaacgc catcctggcc cgtgacacgg
    46501 caaaggtatc tactctggat ctgattttta ccgtcctcac cagcccggat gccgcattag
    46561 caacttactg gggccatatg ccggataccg tcacggcgga aaacggtgct gtctttgagc
    46621 gccctaaact gtggaaagag ctgccgtcga catccggggt aagtcagata acgaatagca
    46681 atgaagcctg gccagtgttt aacgcaacgc agagggctga cagcaatctc agcccttgtg
    46741 aagcggcgcg tcgaccattg agcgatgatt tagaagcgct ctatacacgt tatccaggaa
    46801 acacgattac cgcgcaaatt ggctggccta cgcagtatgc atggtgggcg gtggataact
    46861 gggaaggggg cagtaatccg caggccgtca gcctggtaaa tggttcgaga ggccaatata
    46921 atcccgctca tcaggcctgc ctggttaacc cgcgtgcgag cgtttccagc gtcacgctga
    46981 catcaacggc gctggatgac gccacacagg cggcgacggt gaaaaaaggc gacgccgcgc
    47041 ccattgtcgt taccgtaaaa gacagcgccg ggaagccggt gccgaatatc gcttttatcc
    47101 taaaacgcgg tgaggccgtg ccgcgtaaca gcggggcgac gctgtatggc gatgtggatg
    47161 cgatggacga tttgactgtg caaccttcat ccggtgcggc agtcacgttg gctgacagtg
    47221 gaaataccat cgacggagtc acgggagcgg acggtacggc aagctttacc gttcgtcagg
    47281 ataatacgcc aggatataaa acgccgttga cggtgacgct gacggataac gccaccataa
    47341 cggcgacgct ggacacgatt ttcaccgtgc cgaccagccc gaatgtcgcc acggcgtatt
    47401 tctggggtca tatggcggat accgccacgg taagtggcaa gacgctgcat cgcccgctat
    47461 taaaatcgga gttgccgtca ggcgctaccg cagcagcaac gcctgatgta aataatgaaa
    47521 cctgggcact ggcgcatgtt atggattcct ctaaatggga tgtcgcgcag cagtgtggca
    47581 gtatgaataa cgtgccgagc agtgccgaat tacaaacatt acactccggc tttagcacat
    47641 tgggatggcc gtcctcgata agcttcccgt atttatctac tgataaggct gggtcgttct
    47701 attgtggtgt tgatgaaggc tctggcagtc tcaactgtgg cattcagcct gcgaaaacgc
    47761 cggggtttgc aacctgcttc cagtaacctt atccaccttg tcaatatgag gttccgaaag
    47821 cctgatacgc aacgcgctat caggcttttt tacgtggctt gcggcttttc gtcaccgcac
    47881 tcacttcact gatcactacg ccatcgccca ggcgagtggt cagtgtctca cccactttca
    47941 cctgcttaac ctgttttaac accgcgccat cagcggcgct ggtaacgcta taaccgcgag
    48001 cgagtgtgga cagcggactc accgcttcga gatgcgttac tgcattaccg aaacgttcac
    48061 gcgtggcgct aagctgtgcg cgcagggttt ctgctaaacg atattccagt tgctgaatgc
    48121 gcgtttgcgc gcgatgaatc ttcggctgtg gattttgctg attcagccgc tgtgttaatc
    48181 gctgctggtg ttgcccggca cgcttaagct gattttccag cgcaaagctc atccgttttt
    48241 gcaggcgttc aagcatggtt tgttggcgtg ccagccggag ctgtggatgc tgctgctgca
    48301 agcgatgatg gatctgcgta aaacgacgcg tgcggttggc gagataataa tccatcgcca
    48361 tctccagccg ttgacgggcc gattgcacct ggcgcagtaa ttcttgctga ttgcggctca
    48421 ctacttcagc ggcggcagac ggcgttggcg cacgcagatc agcaacaaag tcggcaatcg
    48481 tcacatccgt ctcatgcccg acggcactca ctaccggaat gcggctggca aaaatcgccc
    48541 gcgctacgcg ttcgtcgtta aaactccata aatcttccag cgaaccgccg ccgcgcccga
    48601 cgattaatac gtcacactcg ttgcgctgat tcgccagttc aatggcgcga acaatttgcc
    48661 ccggcgcgtc atcgccctga acggcggtgg ggtagatgat caccggcaaa gacggatcgc
    48721 gacgttttaa cacatgcaaa atatcatgta gcgcagcacc ggtttttgag gtgatcacac
    48781 caacgcaatg cgcaggggag gggagtgaat ttttgtattg cagatcgaac aaaccttcag
    48841 cctgcaactt cgctttaagc tgttcgtact tctgttgcag caacccttca ccggcaggct
    48901 gcatactctc gactataatc tgataatcgc cgcgcggttc atagagtgta atattggcgc
    48961 gcactaaaac ttgttgtccg tgctgcgggc gaaaggtcac ccgacggttg ctgttgcgga
    49021 acatcgcaca gcgcacctgg gcggtgtcgt ctttgagcgt aaaataccag tgaccggaag
    49081 ctggttgtgt gaaattagag atttcgccgc tgatccacac ctgtcccatc tcatgctcaa
    49141 gcagcagacg aaccgtttga ttcaggcgac taacggtaaa aattgcaggg gattgagaag
    49201 gtaacatgtg agcgagatca aattctaaat cagcaggtta ttcagtcgat agtaacccgc
    49261 cctccgggga tagcaagcat tttttgcaaa aaggggtaga tgcaatcggt tacgctctgt
    49321 ataatgccgc ggcaatattt attaaccact ctggtcgaga tattgcccat gctacgtatc
    49381 gctaaagaag ctctgacgtt tgacgacgtt ctcctcgttc ctgctcattc taccgttctg
    49441 ccgaatactg ctgacctcag cacccagctg acgaaaacta ttcgtctgaa tatccctatg
    49501 ctttccgcag caatggatac cgtaacggaa gcgcgcctgg ctattgctct ggctcaggaa
    49561 ggcggtatcg gctttatcca caaaaacatg tccattgaac gccaggcaga agaagttcgc
    49621 cgtgtgaaaa aacacgaatc tggcgtggta actgatccgc agaccgtgct gccgacaacc
    49681 accctgcgtg aagtgaaaga actgaccgag cgtaacggct ttgcgggcta cccggtggtt
    49741 accgaagaaa acgaactggt cggcatcatc accggtcgtg acgtgcgttt tgtgaccgat
    49801 ctgaatcaac cggttagcgt ttacatgacg ccgaaagagc gtctggtaac cgtgcgtgaa
    49861 ggcgaagccc gtgaagtggt gctggcaaaa atgcacgaaa aacgcgttga aaaggcgctg
    49921 gtggttgatg acgaattcca cctgatcggc atgatcactg tgaaggactt ccagaaagcg
    49981 gaacgtaaac cgaacgcgtg taaagacgaa caaggccgtc tgcgtgtagg tgcagcggtt
    50041 ggcgcgggtg cgggtaacga agagcgtgtt gatgcgctgg ttgccgcagg cgttgacgtt
    50101 ctgctgatcg actcctctca cggtcactct gaaggtgttc tgcagcgtat ccgtgaaact
    50161 cgcgctaaat atcctgacct gcaaatcatc ggcggcaacg tggcaacagc agcaggtgcc
    50221 cgcgcgctgg cagaagccgg ttgcagtgcc gttaaagtgg gtatcggccc tggttctatt
    50281 tgtactaccc gcattgttac tggcgtcggt gttccgcaga tcaccgccgt tgctgacgca
    50341 gtagaagccc tggaaggcac cggaattccg gttatcgctg acggtggtat tcgtttctcc
    50401 ggcgacatcg ccaaagctat cgccgctggc gcaagcgcgg taatggtggg ttccatgctg
    50461 gcaggtactg aagaatctcc gggtgaaatt gaactctacc agggccgttc ttacaaatct
    50521 taccgtggta tgggttccct gggcgcgatg tccaaaggtt cttctgaccg ttacttccag
    50581 agcgacaacg ctgccgacaa actggtgccg gaaggtatcg aaggtcgcgt ggcctataaa
    50641 ggtcgcctga aagagatcat tcaccagcag atgggcggcc tgcgctcctg tatgggcctg
    50701 accggctgtg gtactatcga cgaactgcgt actaaagcgg agtttgtacg tatcagcggt
    50761 gcgggcattc aggaaagcca cgttcacgac gtgaccatta ctaaagagtc cccgaactac
    50821 cgtctgggct cctgattctc ttcgcccgac ttcatgtcgg gcgatttata ttatctgttt
    50881 cacttgcctc ggaataagcg tcaatgacgg aaaacattca taagcatcgc atcctcattc
    50941 tggacttcgg ttcccagtat actcaactgg ttgcgcgccg cgtgcgtgag ctgggcgttt
    51001 actgcgaact gtgggcgtgg gatgtgacag aagcacaaat tcgtgacttc aatccaagcg
    51061 gcattattct ttccggcggc ccggaaagca ccaccgaaga aaacagcccg cgtgcgccgc
    51121 agtatgtctt tgaagcaggc gtaccggtat tcggcgtttg ctatggcatg cagaccatgg
    51181 cgatgcagtt gggcggtcac gttgaagcct ctaacgaacg tgaatttggc tacgcgcagg
    51241 ttgaagtcgt aaacgacagc gcactagttc gcggtatcga agatgcgctg accgcagacg
    51301 gtaaacctct gctcgatgtc tggatgagcc acggcgataa agttaccgct atcccgtccg
    51361 acttcgtcac cgtagccagc accgaaagct gcccgtttgc cattatggct aacgaagaaa
    51421 aacgcttcta tggcgtacag ttccacccgg aagtgactca cacccgccag ggtatgcgca
    51481 tgctggagcg ttttgtgcgt gatatctgcc agtgtgaagc cctgtggacg ccagcgaaaa
    51541 ttatcgacga tgctgtagcc cgtatccgcg agcaggtagg cgacgataaa gtcatcctcg
    51601 gcctctctgg tggcgtggat tcctccgtga ccgcaatgct gctgcaccgc gctatcggta
    51661 aaaatctgac ttgcgtattc gtcgataacg gcctgctgcg tctcaacgaa gcagagcagg
    51721 ttctggatat gtttggcgat cactttggtc tgaacattgt tcacgttccg gcagaagatc
    51781 gcttcctgtc agcgttggcc ggcgaaaacg atccggaagc aaaacgtaaa atcatcgggc
    51841 gcgttttcgt tgaagtattc gatgaagaag cactgaaact ggaagacgtg aagtggctgg
    51901 cgcagggcac tatctaccct gacgttattg aatctgccgc atctgcaacc ggtaaagcac
    51961 acgtcatcaa atctcaccac aacgtgggcg gcctgccgaa agagatgaag atgggcctgg
    52021 ttgaaccgct gaaagagctg ttcaaagacg aagtgcgtaa gattggtctg gagctgggcc
    52081 tgccgtacga catgctgtac cgtcacccgt tcccgggacc aggccttggc gttcgtgttc
    52141 tgggtgaagt gaagaaagag tattgtgacc tgctgcgccg tgctgacgcc atcttcattg
    52201 aagaactgcg taaagcggac ctgtacgaca aagtcagcca ggcgttcacc gtcttcctgc
    52261 cggtacgttc cgttggcgta atgggcgatg gtcgtaagta tgactgggtt gtctctctgc
    52321 gtgctgtcga aaccatcgac tttatgaccg cacactgggc gcatctgccg tacgatttcc
    52381 tcggtcgcgt ttccaaccgc attatcaatg aagtgaacgg tatttcccgc gtggtgtatg
    52441 acatcagcgg caagccgcca gctaccattg agtgggaatg attaacagct aactcatagc
    52501 aagtcattcc tattcagatg ctaattcaac cctctgtttt tacagagggt ttttgttttt
    52561 atgtattcat ttctattcac tctacaccat atttttcggc ggtacaggtg acggtattac
    52621 cttaaaggta tactctcata ccgtcatgaa aatggtttct atacgggtga attgtgctta
    52681 ccgatacaaa attaaaaaac ctcaagccgc aggacaaact gtacaaggtc tccgatcgtg
    52741 acgggctgta tgtagctgtg cttacgtcag gcacggtctc gtttcgctat gactaccgta
    52801 tcaacggtcg ccgcgaaaca ctggtaatcg ggcagtatgg gcgtgacggt atcagcctgg
    52861 cagaagcgcg agaagaactg attgctgcaa agaagctgct taaagcaggc cagtcaccgg
    52921 ctgcggctaa acgtgacggt atcaaaaaga ttcgtggtgc cgagacgttt gcggtacata
    52981 ccgacagtta tatgaaacac gtcatcctgg ctgacagtac ccgcgcaatg aaacaggcgg
    53041 tgatcgaccg tgacatactt ccggttcttg gcaataaaat gatggctgaa attaccacat
    53101 cgatggttcg tgatttgtgt gaccggattg tcgaacgcgg tggccgggca acagcagtgc
    53161 aggccaggga gatcatcagt agcgtatacc gtcacgccaa tgaccgtggt catggtttgt
    53221 ttaatcctgc ggctgacatt aaaccttcgt ctatcgccat atttaaacca cgagagcgaa
    53281 cactgacacc agaagaaatt ggcctgttct tccgcacgct ggatgccatt ggtgctatgg
    53341 gcactatgaa aatggcttta aagctggtgc ttatcactat ggttcgtaaa ggcgaattca
    53401 ctaatgcaac gtgggacgaa atagatttta aaaaatggac atggacaatt ccttcagacc
    53461 gcatgaaggg aagccgggcg cacgttattt acctgcctaa acaggctcag gatatattgg
    53521 ttgggttgca gatgtgcgct ggtggaagtg aatatctggt tcctggtcgt tacaacttcc
    53581 ggaagccatt atctaatgcc gcgctgaact ctctgatcga cagaacggtg aaaataataa
    53641 atgaagatgg tgagcatatt caggacttca ccgtacacga tatgcgccgt acagccagta
    53701 cgttgttgca tgaggctggt tatccttcag actggattga aaaggctctg gcacatgagc
    53761 agaaaggtgt gcgcgccgta tataacaaag cggaatacgc cagacagcgc gcctacatgt
    53821 tgcagcagtg gtccgatatg attgattcct ggattaacgg ggagcatacg gatctgattc
    53881 cgttctcccc gtcgaagttt gagaagtgga tggcggggga ataattttgc ccggcacatc
    53941 cctgtgccgc cgccgtcaga agaatcctgc cttgtcgttg atgtactccg cgtgcgtctg
    54001 gatatcacgc aggcatttgc tcacaccgac gatgtagcag aacatggtgg tcagctccgc
    54061 cgccgcgccc gatacgtcgt gcccgtcttc ctgtaactgg ttcagcagat tcatcagcag
    54121 tgagttctcc gtcaggccga gaacaccaga cggagaatga atcagactgc ggtagccggg
    54181 cttcagtggg gcgctgtatt cttttttgtc ttccagcttg atcgcctcca taatggcggg
    54241 catgaagcct gacaagacct tctccacttt tgtttcttgt tgtcttaaac gtttctcgca
    54301 ttcaatgaag tagcgtcgta cctgacgacc tttttcgtta cgctcgacca ttgctagttc
    54361 tttggctgta tcgagggtga ggtggtactc tttgcggttg tggccgcctc taccagatgt
    54421 ttgctttccc aaattggaaa gcaaaatata gtcttgattt tcaatgaatt cgtattctga
    54481 aatgcgattt gtaatccatg ctgcaaacac ctttttaaca cctaaaaaag cgtgcagatc
    54541 gcgggcattg caaagtaggg ctgtttcgtt agatatagtg ccgttgaata cggggatgag
    54601 ttgactggtc atgatgacct cctttgaagt tttagttagc aatcaccagt tagtagctgg
    54661 tgatcgggtg tcaactagag ccttcaaaga cgctccgggc atattcccct tttgggtatt
    54721 gtattacgcc tctccacccg acctttgtac ggatgtgact atgccaaatt gcaggcataa
    54781 aaaagccgca aagctatcgg gtgcgggtga ccgctttgaa gttctagtgc ggtcagtatg
    54841 cgatagctct ggcggcattg tcaaatcatg cgccactgag tccagcaatc tctgcaatac
    54901 caatagcgag ataatcgcga ttcgtcaggc gtgattttat tgcatatgtc ttagctccac
    54961 tgaagttgtg attttatatg ggttgccttt tacctcaata acacgatgag gatcattttt
    55021 tagctcttcc gtaatcttcc gtaatcttct aacagctgaa cttcggtgtt tataacggta
    55081 agttacagtt tcttttctct gcaatacctc atcattgcgg tacttagtca aaaccatgac
    55141 gacttcaaac ctgacgccgt tgtgtgcaac cataatctgc ttcatgctgc acgctcccgc
    55201 ccctggttgt ctgttggtga caccggagca ttgctgaatg catttgttaa tccgccaata
    55261 tccaacgcgt atccagggtg tagttgcact gccgggtctt cgcactgatt accccaaaca
    55321 tcgaagccat gagacgtctg gcgggcgaac agttcaatgc gagaaacatc gcctaacaat
    55381 tgcacaagtt tttcacgaac gacatctggt tttcttgaat gctcaagccg cggtgcggta
    55441 aatgactgaa cgatccctgc attaatgcgt ggaggtagtt ttccctttac tgcaaacagg
    55501 caatcttcac tattggcgcg agtcatgtgt cccataccca taaccagttt atctggttgt
    55561 cgactaccac attttatcca cgtgaagccc ttcatggtca tcagacggaa tccccaggct
    55621 tcaacaactt ttagcgcttc gagtggttgt gttggcaccc accacatggc caacagacag
    55681 ttttcatcgg ccaaatccca cacaggaagg cggcagatat ccatcacact cataaccgga
    55741 tatttaaaac tggcaccgcg attaccatct gcggctttgt cccggtatac ccagggtgga
    55801 tctgcataga ttagtgtgta tttcttagtc ataaaccacc ccacaacatc ctatgccgct
    55861 atagtcgcca cggcgaaggc cgttaccttt tgtgatacat tggtccctgc gaaccgcgat
    55921 ccttgcacgc tcaacatcac cagaagcaac atccatacac tgaagccaaa ggtgggcggc
    55981 aatgcggaac tgcccttttt tctctctttc aatcgcgcgt ttttcgatct ctattgccgc
    56041 aggagtaacg gcaacaacct ttgaagggct gcgcattgaa accttgttca tgtgatattt
    56101 ttcaagtcgg cttaactttc tcacttaatc caaccctctc tgaaaattaa tgccagcaga
    56161 taaagccatg ctgaaacaga ggccaggaat aagtaccatc ctgaccattt gctccagtgc
    56221 cttagcagca cactcatgca gcgttgctca caggacgata tacacgttgc tgaacaggag
    56281 gctttttacc ctggaactct gccgggcttg ctgcctgacg ttcatcaagc caacgctcaa
    56341 cttcatcacg gttccatgcg cagcgtttat cggtgatata ccagcgttta ggaaattccc
    56401 ctgcgcgctc catacggtcg atagtgctcc atgacagtgg caccaccgcc aggagttcct
    56461 tcttacctaa tgcacctttc ataaatacct ctcttggttg cagtgcggcg cgcgtggcgc
    56521 cgcggtggtg gttacataga tgtttcgttt aattcttccc gacgaacgct gtaaacgtcg
    56581 gtggcttttg ccagcagttc gtcatcatct gaaagttttt gtgcaatgta tttgtaagcc
    56641 ttatccagtt cggagacagt gctgtaattc atcgctgcgc tggtaaaggc catcagcatt
    56701 tcttctggct cacggctatc cgctttacgc gtttgctcat caggcttttt cactgattta
    56761 gcgttgatca gactgttcat tcccgcagcc gtagtcattt gcggagtaat gtctcgctca
    56821 acgcgcggtg ccgtttcctg taattcgtca ggggtgtaaa caccgagaag tacatcagga
    56881 gcgtgcaggc gagcccatcg tttcgtgcaa agataggcaa gttgctggcg cggatcctgt
    56941 tcccacaatg gagagttacg cactcctgct tgcgccatac tgatggtaag ctcacgtggt
    57001 tctgcttctc ctttaagaac tgctgacaca gttaccgtca gattcggtga tttatctgtt
    57061 ttgccgttaa cattcgacca gtcaccgctc cagcgataat tcaggcgtgt cgccagcagg
    57121 ctggaagagg atacgaccgc gtttaccaac tgtgcttcgt agcctaacgt tccgtttacc
    57181 acatgcgttt tctgcgccac ggcgaaaggg ttcattcccc actgtgccgc ctgcatggtc
    57241 accgccaggc aatcggcagg tttgccttca agatgtttcg gtacagtcgc tttgctttgt
    57301 gacatcaact ccgcgaaacg caccagttga ttcatgccct cggggctgaa gattgccgca
    57361 gcagtgccta cagttgcgcc tggttgtgat gtgattgcga tatcattgct catacgtaca
    57421 tatcctgttt acgtgcccag tcagggcgtt taataatttc cactccgccc cattcatcgt
    57481 tgatgcggca ttcgtgatag gtattcagat cccggcggaa cagagcgtgc ccggcatcga
    57541 catccggcgc atccagctcg aacacgcgta ccggataccg accacaatca atgctttcgc
    57601 tcacggcaag aaagaaaaaa ccatgtggct gaccagtaac cctcattgcg ccttcgcggt
    57661 acattgcgtc ctgcacgtgg tagcggaact cctcgatgtg gcgtgcaaaa cggtccatat
    57721 ctgtaacctt tttcacgtcg acgatcacgt tgtgctcttt cagccatttg tctggacgaa
    57781 tgcggcacaa ctcaccagtc tcttcatcgt tccagtacat tgatgcttcg cagtaaccag
    57841 gtgcttccag catccagcgt gccgccgggt gagccattgc gctatcacgc atcagctcca
    57901 gtttccgcca ctgctcggca tcaagtaccg taatccccat atccgccaca tcacgaagaa
    57961 atgcctcttc gtcagcttta ccttgtttcg tccgacgatc gaatttcggc gaaacaatga
    58021 agcgtttgtc gaactctcca ggctccagaa gcagacagtg caatgcggtt cccatatcca
    58081 gtgcagactt tttctcttcg tcttctggtg ctgcctgaac ccatttaaga agcgccggat
    58141 tcttggcaac catgtccagt tgcgacttac tcacgccgtc cccggcgtgg tagtcttcgt
    58201 tgctgatgtc gaaataaatt cccggcttca tgccgcgtcc ctctgcccat caagctgatc
    58261 agccagatcc cagcgggcga taattgccat tgcctcgcgc cggtaggaat ccatcagttc
    58321 ttcgaactca gggctgtctt tagctgcttc cagcacttcc tggcgaactc ctttgcctgt
    58381 tacaacgtcg aaagttgagg ccagttgatg aagccggatg ctctcaatca gttcaacttg
    58441 tcggtcatat agctgttctg acaggcggta gtccttgtcg aatgccagca tgattttttg
    58501 aagatttttc tgctgattaa cgttcattat cagccctccc atatctcgtt atcgttggcc
    58561 acatcgcgag cttctttgct gacgaaagcc cacttaatac cttcctgtaa ggtgcggaac
    58621 ttccagctca tgaatccgca tgcagtaacg cagtaccaac cgttgatgat tttccactgc
    58681 ataacttgtt acctcggttt gttaccgttg aggtaatgat tatgcgtatt tggtttggtg
    58741 tcaatagata tgagttaaaa aaattaccca caaggtaatc ttgctggcaa taaaaaagcc
    58801 gccatgaggc ggcttacttg ctgaaaacta tagttttatt gtttgctttt ttcgttctgg
    58861 ttgatgacaa attcaatgta actttcgatc tttgctttct cggtttcggg taacaatgcg
    58921 tagcgcgagc ggtcatagtt gatagtcgca gggtcgtgcg ggtgaatcag taattcatag
    58981 ccgtgacgcc cgaatgcgga tgcaacattc tccagggtgg aaatggaaac gctgacctca
    59041 ttgtttaaca ggcggctgat tgtcacctgg gcgacgccgg atgcgcggtg aagttttccc
    59101 tgtgttgaaa ggtcgcggct ttcgctcatc cagcgttcca ggttgtgagc cgccagctga
    59161 ccaatgtcgc ttgggccgac aggctgaaaa ccttcctgag aaagcgagcg atcgatatca
    59221 agccagttac ggggtttatt ggcggcagct tcaatttttc gcgcaacctg gtcgccgata
    59281 accttcttac caagagccca gcggtttacc agatttgcct gagttccaag tttttctgcc
    59341 atccgcgtct gaacaccatt gaattcacgg tcgatcaagt cgttgagatt ttgcctgcgg
    59401 acgtcctgga tacttttcat tttctggaaa atcgcctcat atatgaatca gtagatgatt
    59461 caatttaaag caatattacc caacaggtaa atgcacccca taggtaacta tccttgattt
    59521 ttgttacctt atgggtgaat atttattatc tgaaataaat atcaggcaat agctatgagc
    59581 gataacggac atttcgattt caaaaagcac tggcttgcac ttactccgga tgagcgtgaa
    59641 gccttcgcac aggaagccgg aacgacgagt cactatatac agactcactt aacaggtaag
    59701 cgcaaaatgc caggtaaggt attgatgaat gggcttttta aagcctgtaa aacaagacaa
    59761 tggctgcgct caaaagcaga actggcatac ttcttctact catgatatcc agccacaacc
    59821 ctctgtagac cgccacccgg cggtcttttc atatctattc gtacctcaaa ggtaataaaa
    59881 aaccaaatct ggttgatcaa attttccaat tgtgcaaaat agccaatatc aataacaaaa
    59941 aggggcggaa aaattgaaga tagtaaccag aatggaggcc gcaaaagccg ggttaaatcg
    60001 ctatttcaca ggaaagcggt gccgtcacgg ccatctctct gaaaggtatg ttctgaacgg
    60061 aacatgtgtt gaatgtgcaa tgaatagcgc caaccgccat cgtaatgaat ttgcttgtgc
    60121 actaaagagt gcaagagggg aaacctatgg caagcagctg gattaaggtt gaagttatca
    60181 ctcctgataa acctgaaatt tttcagatag cagaaattct gggtattgat ccagatgctg
    60241 ttcttggaaa gctggttcgt atatgggcat gggctgacca gcaaacaata gacggtaacg
    60301 ctggcagcgt tacaaaagga gtacttgata gactcgcttt tattacagga tttgctgacg
    60361 ccctcattag cgtcggatgg cttgcttatc atgacggcaa actaattctt ccaaactttg
    60421 agcgacacaa tggagaatca tcgaaaaaac gtgcacttac gaatagaaga gtggcagagc
    60481 atcgaaaacg agtaacgcaa aaagtaacgc caacagcgtt acaaaaggag ttaccagagg
    60541 aagaggaaga ggaagatata tataaaaccc cacacatagc gcacgtgcgc gagggtgctc
    60601 cgaccagtga agcgaacagt acgccgttgc aggtggcaga acctgaattt ctggatggcc
    60661 tgagtgaacc catcgggaaa tttccgatga cagatggctg gctaccgtcg ccagattttc
    60721 ggcggcgtgc tgcgctgtgg ggaacggccc tgccggaacc ggaatttaca cctgctgaac
    60781 ttgctgcatt ccgggattac tggatggctg agggcaaagt gttcacgcag gttcagtggg
    60841 aacaaaaatt cgccaggcac gtaaatcata tcaggggaaa atcaaaaaat gccgggaaaa
    60901 gcgatgagct tgactggaat aacactgact ggatagaagg ggtgtgggat gaaatcaact
    60961 ccagaacttc tcaatgagta cgatcgctta cgtgagcatg gtgttgctgt gcatgaagag
    61021 cggcgtgaca gcaatggcaa aaaggagcag gttgctagaa ttttcaatga actatttgtc
    61081 cagttacagg ctgcatttcc tgcaagcgtt tcgaccataa gggagcagaa caaacttaat
    61141 gaattccgta agcaatggat gcttgcgttt ctggagaatg ggatcactac aatggaacag
    61201 gttaacgctg gtatgcgcca cgcccgcgcc agtgaatctc cgttctggcc gtcgccaggg
    61261 caattcatca agtggtgcaa agacagcaag atggttcttg gcgtcaccat tgacgatgtg
    61321 atggcggagt ttcaccggta cagcaaggaa aaaagtttat atcctggtgg tcccgaaaga
    61381 ttcccgtggc gacatccggt tatgtactgg gtcgtatgtg atacccgccg tgcaatgtat
    61441 cagcgccagc ttagcgagat tgaggttgag aaacacgcgc gcaggctgct cgatgattgg
    61501 gcgaaaaagg tggcttccgg acagcagata cccgatccgg tgatcagcat acaggcaaag
    61561 ccagaaccca tgagtacgcc tcctgacact gggagagacg tttaccatcc accagggcga
    61621 agtttcgggt gcatgcctaa cgccgccacc ctggggggaa taacaccggc gcagtggctg
    61681 atagaggaat acaggcgggg aaaggcggca ggatttatca agtaatacca gcgcgatagc
    61741 gcattttttt acgcctacat gattacctgt tgggtaacaa aatgttctaa tctctattga
    61801 tttcatgtct tatgtggttt ttaattacct aaggggtaaa tcatgagaaa acagatacag
    61861 gctcttggtc gactcaaaac aggccaaatg aacaaaacag aatctgcgta ttgccagcac
    61921 cttgagctgc gtaaacgtgc aggagaaatc gtctggtatc gatttgaggg tatcaagctg
    61981 cggttagctg acaacacgtt ctatacgccc gattttgctg tgatgctcgc caccggcgag
    62041 atggaactgc acgaagtgaa aggtttctgg accgacgacg ccagggtgaa aaccaaagtc
    62101 gccgcagatc agtatccgtt ccgaatcatc ggggtaacgg ttaaaccaaa gaaagcaggt
    62161 ggtggctgga acatcgaaga gttctgaagc gacgatcttt ttagttatca atgtaatcaa
    62221 taagttatgt ggataagcga gggtaaagat ggaaagtaat atcaaagggt tagttgccgc
    62281 cgggcatgag atggcttcgg aactgaaagc agaatgtggt gccgttgata tgcgcagtgt
    62341 ggcaaagctg atcagcgatt tggcaacgca actggaagtg caactggtgc gtgctaatgc
    62401 gct
//
LOCUS       DAMZFT010000012        60321 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.012, whole genome shotgun sequence.
ACCESSION   DAMZFT010000012 DAMZFT010000000
VERSION     DAMZFT010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 60321)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 60321)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..60321
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.012"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(23..241)
                     /gene="ogrK"
                     /locus_tag="PN977_000874"
     CDS             complement(23..241)
                     /gene="ogrK"
                     /locus_tag="PN977_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage transcriptional regulator OgrK"
                     /protein_id="HDI6040937.1"
                     /translation="MFHCPLCQHAAHARTSRYITDTTKERYHQCQNVNCSATFITYES
                     VQRYIVKPGEVHAVRPHPLPSGQQIMWM"
     gene            complement(323..1486)
                     /locus_tag="PN977_000875"
     CDS             complement(323..1486)
                     /locus_tag="PN977_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016157990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage late control D family protein"
                     /protein_id="HDI6040938.1"
                     /translation="MLDALTFDAGSTLTPDYMLMLDSRDITGNISDRLMSMTLTDNRG
                     FEADQLDIELNDADGQVELPVRGAVLTVYIGWKGFALVCKGKFTVDEVEHRGTPDVVA
                     IRARSADFRGTLNSRREGSWHDTTLGAIVEAIASRNRLEASVAPSLAGIKIPHIDQSQ
                     ESDAKFLTRLAERNGGEVSVKMGKLLFLKAGQGVTASGKKIPQITITRSDGDRHHFAI
                     ADRGAYTGVTAKWLHTKDPKPQKQKVKLKRKKKEKHLRALEHPKAKPVTQKKAPKVPE
                     AREGEYMAGEADNVFALTTVYATKAQAMRAAQAKWDKLQRGVAEFSIILATGRADIYT
                     ETPVRVSGFKRVIDEQDWTVTKVTHFLNNSGFTTSLELEVRLSDVEYETEDYE"
     gene            complement(1486..1965)
                     /locus_tag="PN977_000876"
     CDS             complement(1486..1965)
                     /locus_tag="PN977_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000978923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDI6040939.1"
                     /translation="MMLALGMFVFMRQTLPHQTMQRESDYRWPSNSRIGKRDAFQFLG
                     VGEENITLAGVLYPELTGGKLTMTTLRLMAEEGRAWPLLDGTGMIYGMYVISKVSETG
                     SIFFADGTPRKIDFTLSLTRVDESLAALYGDIGKQAESLIGKAGSLATRFTGMTGAE"
     gene            complement(1980..4427)
                     /locus_tag="PN977_000877"
     CDS             complement(1980..4427)
                     /locus_tag="PN977_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005228341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HDI6040940.1"
                     /translation="MSNNVKLQVLLRAVDQASRPFKSIRTASKSLSGDIRETQKSLRE
                     LNGQASRIEGFRKTSAQLAVTGHALEKARQEAEALATQFKNTERPTRAQAKVLESAKR
                     AAEDLQAKYNRLTDSVKRQQRELAVVGINTRNLAHDEQGLKNRISETTAQLNRQRDAL
                     ARVSAQQAKLNAVKQRYQAGKELAGNMASVGAAGVGIAAAGTMAGVKLLMPGYEFAQK
                     NSELLAVLGVAKDSAEMTALRKQARQLGDNTAASADDAAGAQIIIAKAGGDVDAIQAA
                     TPVTLNMALANRRTMEENAALLMGMKSAFQLSNDKVAHIGDVLSMTMNKTAADFDGMS
                     DALTYAAPVAKNAGVSIEETAAMVGALHDAKITGSMAGTGSRAVLSRLQAPTGKAWDA
                     LKELGVKTSDSKGNTRPVFTILKEMQASFEKNRLGTAQQAEYMKTIFGEEASSAAAVL
                     MTAASTGKLDKLTAAFKSSDGKTAELVNIMQDNLGGDFKEFQSAYEAVGTDLFDQQEG
                     ALRKLTQTTTKYVLKLDGWIQKNKSLASTIGIIAGGALALTGIIGAIGLVAWPVITGI
                     NAIIAAAGAMGAVFTTVGSAVMTAIGVISWPVVAVVAAIVAGALLIRKYWEPVSAFFG
                     GVVEGLKVAFAPVGELFTPLKPVFDWLGEKLQAAWQWFKNLIAPVKATQDSLNSCRDT
                     GVMFGQALADALMLPLNAFNKLRSGIDWVLEKLGVINKESDTLDQTAARTQAATYGSG
                     GYIPATSSYAGYQAYQPVTAPAGRSYVDQSKNEYHISLTGGTAPGTQLDRQLQDALEK
                     YERDKRVRARASMMHDG"
     gene            complement(4420..4539)
                     /locus_tag="PN977_000878"
     CDS             complement(4420..4539)
                     /locus_tag="PN977_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001496926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GpE family phage tail protein"
                     /protein_id="HDI6040941.1"
                     /translation="MADVAVIFHWPPSELYPMSLTELITWREKALRRSGNTNE"
     gene            complement(4572..4847)
                     /locus_tag="PN977_000879"
     CDS             complement(4572..4847)
                     /locus_tag="PN977_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001719230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein"
                     /protein_id="HDI6040942.1"
                     /translation="MNKENVITLDNPVKRGEQVIEQVTLMKPNAGTLRGVSLAAVANS
                     EVDALIKVLPRMTAPMLTEQEVAALELPDLVALAGKVVGFLSPNSVQ"
     gene            complement(4904..5422)
                     /locus_tag="PN977_000880"
     CDS             complement(4904..5422)
                     /locus_tag="PN977_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001554312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major tail tube protein"
                     /protein_id="HDI6040943.1"
                     /translation="MAMPRKLKLMNVFLNGYSYQGVAKSVTLPKLTRKLENYRGAGMN
                     GSAPVDLGLDDDALSMEWSLGGFPDSVIWELYAATGVDAVPIRFAGSYQRDDTGETVA
                     VEVVMRGRQKEIDTGEGKQGEDTESKISVVCTYFRLTMDGKELVEIDTINMIEKVNGV
                     DRLEQHRRNIGL"
     gene            complement(5435..6625)
                     /locus_tag="PN977_000881"
     CDS             complement(5435..6625)
                     /locus_tag="PN977_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001483446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath protein"
                     /protein_id="HDI6040944.1"
                     /translation="MSDYHHGVQVLEINDGTRVISTVSTAIVGMVCTASDADAEIFPL
                     NKPVLITNVQSAIAKAGKKGTLAASLQAIADQSKPVTVVVRVEDGTGDDEETKLAQTV
                     SNIIGTTDENGQYTGLKALLAAESVTGVKPRILGVPGLDTKEVAVALASVCQKLRAFG
                     YISAWGCKTISEVKAYRQNFSQRELMVIWPDFLAWDTVTSTTATAYATARALGLRARI
                     DQEQGWHKTLSNVGVNGVTGISASVFWDLQESGTDADLLNESGVTTLIRRDGFRFWGN
                     RTCSDDPLFLFENYTRTAQVLADTMAEAHMWAVDKPITATLIRDIVDGINAKFRELKT
                     NGYIVDATCWFSEESNDAETLKAGKLYIDYDYTPVPPLENLTLRQRITDKYLANLVTS
                     VNSN"
     gene            complement(6945..7844)
                     /locus_tag="PN977_000882"
     CDS             complement(6945..7844)
                     /locus_tag="PN977_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000014361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6040945.1"
                     /translation="MSGIRITQAIDNNQILVNVNTFNYSVNPYPLKCPDPNCSAHLVY
                     VKSHIRRSFNKTLHIPAFFRLEKNFTHDKLCQYGTSGLNTIYASDSSHDISRALATGN
                     NIFRIHILDEDDISKLSRKAAAMQANPPSDTTDRVYVKRGRKAPYVKNMDSLREIYEY
                     GKAHPNKRNSIKIVTGTSTVTWSDFFYETTQLERLSTYLQKVKIAQVAVIMKVHVARI
                     PMAKFNYRHFIEGSPMRIKGGFNIYPTIQLGNVTPKLFPLSSIVMVLGKFTIPTNRKM
                     IMDPIFEREVRTIVSSEEQVLIL"
     gene            complement(8060..8587)
                     /locus_tag="PN977_000883"
     CDS             complement(8060..8587)
                     /locus_tag="PN977_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001761879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HDI6040946.1"
                     /translation="MMHLKNIKAGNAKTLEQYELTKKHGVIWLYSEDGKNWYEEVKNF
                     QPDTIKIVYDENNIIVAITRDASTLNPEGFSVVEVPDITSNRRADDSGKWMFKDGAVI
                     KRIYTADEQQKLAELHKAALLSEAESVILPLERAVRLNMATDEERSRLEAWERYSVLV
                     SRVDPANPEWPEMPQ"
     gene            complement(8589..10610)
                     /locus_tag="PN977_000884"
     CDS             complement(8589..10610)
                     /locus_tag="PN977_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016242808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDI6040947.1"
                     /translation="MSTKFKTVITTAGAAKLAAATAPGGRKVNITTMAVGDGGGKLPV
                     PDAGQTGLIHEVWRHALNKISQDKRNSNYIIAELVIPPEVGGFWMRELGLYDDAGTLI
                     AVANMAESYKPALAEGSGRSQTCRMVIIVSSVASVDLTIDTTTVMATQDYVDDKIAEH
                     EQSRRHPDASLTAKGFTQLSSATNSTSETLAATPKAVKTVMDETNKKAPLNSPALTGT
                     PTTPTARQGTNNTQIANTAFVMAAIAALVDSSPDALNTLNELAAALGNDPNFATTMTN
                     ALAGKQPKDATLTALAGLATAADRFPYFIGNDVASLATLTKVGRDILAKSTVAAVIEY
                     LGLQETVNRAGNAVQKNGDTLSGGLTFENDSILAWIRNTDWAKIGFKNDADGDTDSFM
                     WFETGDNGNEYFKWRSRQSTTTKDLMNLKWDALYVLVNAIVNGEVISKSANGLRIAYG
                     NYGFFIRNDGSNTYFMLTNSGDNMGTYNGLRPLWINNATGAVSMGRGLNVSGETLSDR
                     FAINSSNGMWIQMRDNNAIFGKNIVNTDSAQALLRQNHADRKFMIGGLGNKQFGIYMI
                     NNSRTANGTDGQAYMDNNGNWLCGSQVIPGNYGNFDSRYVKDVRLGSQQYYGVNNWQT
                     WNFQCPSGHVLSGINVQDTGSNSADNIAGVYYRPVQKYINGTWYNVASV"
     gene            complement(10621..11151)
                     /locus_tag="PN977_000885"
     CDS             complement(10621..11151)
                     /locus_tag="PN977_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001285325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein I"
                     /protein_id="HDI6040948.1"
                     /translation="MSDSRLLPTGSSPLEVAAAKACAEIEKTPVSIRELWNPDTCPAN
                     LLPWLAWAFSVDRWDEKWPEATKRAVIRDAYFIHCHKGTIGAIRRVVEPLGYLINVTE
                     WWENSDPPGTFRLDIGVLESGITEAMYQEMERLIADAKPASRHLIGLNITRDIPGYLF
                     AGGVAYDGDVITVYPG"
     gene            complement(11144..12052)
                     /locus_tag="PN977_000886"
     CDS             complement(11144..12052)
                     /locus_tag="PN977_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001121472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate assembly protein"
                     /protein_id="HDI6040949.1"
                     /translation="MPIIDLNQLPAPDVVEELDFETILAERKATLISLYPEDQQEAVA
                     RTLTLESEPLVKLLEENAYRELIWRQRVNEAARAVMLACAAGNDLDVIGANYNTTRLT
                     ITPADDSTIPPTPAVMESDTDYRLRIQQAFEGLSVAGSVGAYQYHGRSADGRVADISV
                     TSPSPACVTISVLSRENNGVASKDLLAVVRNALNGEDVRPVADRVTVQSAAIVEYQIN
                     ATLYLYPGPESEPIRAAAVKKLEAYITAQHRLGRDIRLSAIYAALHVEGVQRVELAAP
                     LADIVLNNTQASFCTEYSVVTGGSDE"
     gene            complement(12057..12404)
                     /locus_tag="PN977_000887"
     CDS             complement(12057..12404)
                     /locus_tag="PN977_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005228331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPW/gp25 family protein"
                     /protein_id="HDI6040950.1"
                     /translation="MTARYLGMNRSDGLTVTDLEHISQSIGDILRTPVGSRVMRRDYG
                     SLLASMIDQPQTPALELQIKVACYMAVLKWEPRVTLSSVTTARSFDGRMTVTLTGQHN
                     DTGQPLSLTIPVS"
     gene            complement(12401..13036)
                     /locus_tag="PN977_000888"
     CDS             complement(12401..13036)
                     /locus_tag="PN977_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001093708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein V"
                     /protein_id="HDI6040951.1"
                     /translation="MNTLANIQELARALRNMIRTGIIVETDLNAGRCRVQTGGMCTDW
                     LQWLTHRAGRSRTWWAPSVGEQVLILAVGGELDTAFVLPGIYSGDNPAPSASADALHI
                     RFPDGAVIEYEPETSALTVSGVKTASVTASDSVTATVPVVMVKASTRITLDTPEVVCT
                     NRLITGTLEVQKGGTMRGNIEHTGGELSSNGKVLHTHKHPGDSGGTTGGPL"
     gene            13120..13905
                     /locus_tag="PN977_000889"
     CDS             13120..13905
                     /locus_tag="PN977_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001704934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6040952.1"
                     /translation="MFSDNVTNAWWFISLYLFLLIALTFVTFGKSNLMRFIAHHFNLE
                     YSDRKLKMLDKKWRDIQLFKIINGINVSGIEDVRMIQQGLIDGKLKTSYFFLTRFWGD
                     ITKPPHIIKTTIVILASIIYILFACYIHNEQSAIVRDAIGIPYKNMMYYVYSDKVLLS
                     FKNKTVEFNKTYSLADCKSLQNVFIKDTLPEIACNKLLQLNKEDSEWLSQEIKDNNSH
                     RKTLLIISLTYFISGLLIFLSYTKFLYANKKVLEYKASNKNHS"
     gene            complement(13977..14429)
                     /locus_tag="PN977_000890"
     CDS             complement(13977..14429)
                     /locus_tag="PN977_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001001795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage virion morphogenesis protein"
                     /protein_id="HDI6040953.1"
                     /translation="MNEFKRFEDRLTGLIESLSPSGRRRLSAELAKRLRQSQQRRVMA
                     QKAPDGTPYAPRQQQSARKKTGRVKRKMFAKLITSRFLHIRASPEQASMEFYGGKSPK
                     IASVHQFGLSEENRKDGKKIDYPARPLLGFTGEDVQMIEEIILAHLER"
     gene            complement(14422..14889)
                     /locus_tag="PN977_000891"
     CDS             complement(14422..14889)
                     /locus_tag="PN977_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001406878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDI6040954.1"
                     /translation="MLKPDSLRRALTDAVTVLKTSPEMLRIFVDNGSIASTLATSLSF
                     EKRYTLNVIVTDFTGDFDLLIVPVLAWLRENQPDIMTTDAGQKKGFTFYADINNDSSF
                     DISISLMLTERTLVSEVDGALHVKNIPEPPPPEPVTRPMELYINGELVSKWDE"
     gene            complement(14852..15010)
                     /locus_tag="PN977_000892"
     CDS             complement(14852..15010)
                     /locus_tag="PN977_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019841551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage lysis protein"
                     /protein_id="HDI6040955.1"
                     /translation="MPGSDPQTNGDLSADIRQLENALARCASQVKMIKHCQDENDAQT
                     RQPAQGAD"
     gene            complement(14997..15422)
                     /locus_tag="PN977_000893"
     CDS             complement(14997..15422)
                     /locus_tag="PN977_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001585210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysB family phage lysis regulatory protein"
                     /protein_id="HDI6040956.1"
                     /translation="MSKLMIVLVVLLSLAVAGLFLAKHENASLRTSLDRANNVANGQQ
                     TTITMLKNQLHVALTRADKNELAQVALRQELENAAKREAQREKTITRLLNENEDFRRW
                     YGAGLPDAVRRLHQRPACTDASDCPQRLPESEPLPDAGQ"
     gene            complement(15410..15835)
                     /locus_tag="PN977_000894"
     CDS             complement(15410..15835)
                     /locus_tag="PN977_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000736575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein lysA"
                     /protein_id="HDI6040957.1"
                     /translation="MKKLSLSLMLNVSLALMLALSLIYPQSVAVSFVAAWAILATVIC
                     VVAGGVGVYATEYVLERYGRELPPESLAVKIVTALFLQPVPWRRRSVALVVMVATFIS
                     LVAAGWIFTALIYLVASVFFRLIRTACRQRFEGREPCQS"
     gene            complement(15850..16347)
                     /locus_tag="PN977_000895"
     CDS             complement(15850..16347)
                     /locus_tag="PN977_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016235441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 104 protein"
                     /protein_id="HDI6040958.1"
                     /translation="MPVINTHQNIAAFLDMLAVSEGTANHPLTKNRGYDVIVTGLDGK
                     PEIFTDYSDHPFAHGRPAKVFNRRGEKSTASGRYQQLYLFWPHYRKQLALPDFSPLSQ
                     DRLAIQLIRERGALDDIRAGRIERAISRCRNIWASLPGAGYGQREHSLEKLFTVWRTA
                     GGVLA"
     gene            complement(16347..16628)
                     /locus_tag="PN977_000896"
     CDS             complement(16347..16628)
                     /locus_tag="PN977_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000123123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HDI6040959.1"
                     /translation="MTAEEKSVLSLFMIGVLIVVGKVLTGGEPITPRLFIGRMLLGGF
                     VSMVAGVVLVQFPDLSLPAVCGIGSMLGIAGYQVIEIAIQRRFKGRGKP"
     gene            complement(16632..16835)
                     /locus_tag="PN977_000897"
     CDS             complement(16632..16835)
                     /locus_tag="PN977_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000846400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail protein X"
                     /protein_id="HDI6040960.1"
                     /translation="MKTFALQGDTLDAICVRYYGRTEGVVETVLVANPGLAELGAVLP
                     HGTAVELPDVQTAPVAETVNLWE"
     gene            complement(16835..17344)
                     /locus_tag="PN977_000898"
     CDS             complement(16835..17344)
                     /locus_tag="PN977_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005228322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head completion/stabilization protein"
                     /protein_id="HDI6040961.1"
                     /translation="MMTLIIPRKEAPVSGEGTVVIPQPAGDEPVIKNTFFFPDIDPKR
                     VRERMRLEQTVAPARLREAIKSGMAETNAELYEYREQKIAAGFTRLADVPADDIDGES
                     IKVFYYERAVCAMATASLYERYRGVDASAKGDKKADSIDSTIDELWRDMRWAVARIQD
                     KPRCIVSQI"
     gene            complement(17444..18187)
                     /locus_tag="PN977_000899"
     CDS             complement(17444..18187)
                     /locus_tag="PN977_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase endonuclease subunit"
                     /protein_id="HDI6040962.1"
                     /translation="MTSPAQRHMMRVSAAMTAQREAAPLRHATVYEQMLVKLAADQRT
                     LKAIYSKELKAAKKRELLPFWLPWVNGVLEQGKGAQDDILMTVMLWRLDTGDIAGALE
                     IARYALKYGLTMPGKHRRPPPYMFTEEVALAAMRAHAAGESVDTRLLTETIELTATAD
                     MPDEVRAKLHKITGLFLRDAGDAAGALAHLQRATQLDCQAGVKKEIERLERELKPKPE
                     PQPKAATRTPRKTRSVTPAKRGRPKKKAS"
     gene            complement(18191..19264)
                     /locus_tag="PN977_000900"
     CDS             complement(18191..19264)
                     /locus_tag="PN977_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001248534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein, P2 family"
                     /protein_id="HDI6040963.1"
                     /translation="MRQETRFKFNAYLSRVAELNGIDAGDVSKKFTVEPSVTQTLMNT
                     MQESSDFLTRINIVPVSEMKGEKIGIGVTGSIASTTDTAGGTERQPKDFSKLASNKYE
                     CDQINFDFYIRYKTLDLWARYQDFQLRVRNAIIKRQSLDFIMAGFNGVKRAETSDRSS
                     NPMLQDVAVGWLQKYRNEAPARVMSKVTDEEGHTTSEVIRVGKGGDYASLDALVMDAT
                     NNLIEPWYQEDPDLVVIVGRQLLADKYFPIVNKEQDNSEMLAADVIISQKRIGNLPAV
                     RVPYFPADAMLITKLENLSIYYMDDSHRRVIVENPKLDRVENYESMNIDYVVEDYAAG
                     CLVEKIKVGDFSTPARATAEPGA"
     gene            complement(19323..20177)
                     /locus_tag="PN977_000901"
     CDS             complement(19323..20177)
                     /locus_tag="PN977_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001074418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPO family capsid scaffolding protein"
                     /protein_id="HDI6040964.1"
                     /translation="MAKKVSKFFRIGVEGDTCDGRVISAQDIQEMAETFDPRVYGCRI
                     NLEHLRGILPDGIFKRYGDVVELKAEKIDDDSALKGKWALFAKITPTDDLIAMNKAAQ
                     KVYTSMEIQPNFANTGKCYLVGLAVTDDPASLGTEYLEFCRTAKHNPLNRFKLSPENL
                     ISVATPVELEFEDLPETVFTALTEKVKSIFGRKQASDDARLNDVHEAVTAVAEHVQEK
                     LSATEQRLAEMETAFSALKQEVTDRADETSQAFTRLKNSLDHTESLTQQRRSKATGGG
                     GDALMTNC"
     gene            20351..22123
                     /locus_tag="PN977_000902"
     CDS             20351..22123
                     /locus_tag="PN977_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001357052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase ATPase subunit family protein"
                     /protein_id="HDI6040965.1"
                     /translation="MTITTDTTLLHDPRRQAALLYWQGFSVPQIAAMLQMKRPTVQSW
                     KQRDGWDSVAPISRVEMSLEARLTQLIIKPQKTGGDFKEIDLLGRQIERLARVNRYSQ
                     TGNEADLNPNIANRNKGGRRKPKKNFFSDEAIEKLEQIFFEQSFDYQLHWYRAGLEHR
                     IRDILKSRQIGATFYFSREALLRALKTGHNQIFLSASKTQAYVFREYIIAFARLVDVD
                     LTGDPIVLGNNGAKLIFLGTNSNTAQSHNGDLYVDEIFWIPNFQVLRKVASGMASQSH
                     LRSTYFSTPSTLAHDAYPFWSGELFNRGRASAAERVEIDVSHNALAGGLLCADGQWRQ
                     IVTIEDALKGGCTLFDIEQLKRENSADDFKNLFMCEFVDDKASVFPFEELQRCMVDTL
                     EEWEDYAPFAANPFGSRPVWIGYDPSHRGDSAGCVVLAPPVVAGGKFRILERHQWKGM
                     DFATQAESIRKLTEKYNVEYIGIDATGLGVGVFQLVRSFYPAARDIRYTPEMKTAMVL
                     KAKDVIRRGCLEYDVSATDITSSFMAIRKTMTSSGRSATYEASRSEEASHADLAWATM
                     HALLNEPLTAGISTPLTSTILEFY"
     gene            22123..23157
                     /locus_tag="PN977_000903"
     CDS             22123..23157
                     /locus_tag="PN977_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001752368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HDI6040966.1"
                     /translation="MSKKKGKTPQPAAKKMTASAPKMEAFTFGEPVPVLDRRDILDYV
                     ECISNGRWYEPPVSFTGLAKSLRAAVHHSSPIYVKRNILASTFIPHPWLSQQDFSRFV
                     LDFLVFGNAFLEKRYSTTGKVIRLETSPAKYTRRGVEEDVYWWVPSFNEPTPFAPGSV
                     FHLLEPDINQELYGLPEYLSALNSAWLNESATLFRRKYYENGAHAGYIMYVTDAVQDR
                     NDIEMLRENMVKSKGRNNFKNLFLYAPQGKADGIKIIPLSEVATKDDFFNIKKASAAD
                     LLDAHRIPFQLMGGKPENVGSLGDIEKVAKVFVRNELIPLQDRIREINGWLGQEVIRF
                     KNYSLDTDNG"
     gene            23573..24646
                     /locus_tag="PN977_000904"
     CDS             23573..24646
                     /locus_tag="PN977_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000368931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB N-terminal domain-containing protein"
                     /protein_id="HDI6040967.1"
                     /translation="MDNKIVEIETNKLDFDPKNPRFFRLNDASNAATVIEEMLDDESV
                     HDLMLSIGQQGYFPGEPLLAVKSNGNYIVVEGNRRLAAVKLLNGDLLPPKRKLKGVQE
                     IIDDTTNKPKKLPCIIYENREDVLRYIGYRHITGVKEWDSLSKAKYLKELCDTFYSHE
                     PKEIVLKNLAREIGSKPHYVATLLTALNLYEVAHDHEFFNLPMKASDVEFSYITTALG
                     YSKITNWLGLQDKKDFLDPNLNEENLKRLFSWFFVPDQQGRTIIGESRRIKDIAAVVE
                     KPEAIEILMKSSNLDEAYLYTSGEREALDKALNAASVKLRVVWDMLLKAKELTLEHEE
                     AASEIFEMSKNIRNQIRSKREDD"
     gene            24651..25676
                     /locus_tag="PN977_000905"
     CDS             24651..25676
                     /locus_tag="PN977_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001350076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6040968.1"
                     /translation="MITNLDSMPSNEPYLWADYIEILALTNIDRSFSRGDLYSTLQAQ
                     PEAVLAETDEAEEEGVYDVDDENDTPVRKRTKRSVSRAYTDRKWSYAIGFIRQRIDLF
                     GDSYPFTLSEDNDTVELRDISEKPLEHLERLYLALLICANIKYVNIMSRREITRSFEL
                     ISLPIFESLMPSGSIIKACWASGGQAAPYTGTLYNKFKSIASDIRCTANFKERDFSRG
                     NSGDGGLDIIAWHPMGDQRDAIPISFVQCGCSQEEWEAKQLEASPAMLYSKFPVAHRW
                     ATYYFLPQDLRWIDGEWAHKNKLGDAIFVDRLRLINLTRASDNIDHSQNISYLDIILD
                     PSSAIAA"
     gene            complement(25673..26611)
                     /locus_tag="PN977_000906"
     CDS             complement(25673..26611)
                     /locus_tag="PN977_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001143634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HDI6040969.1"
                     /translation="MPTVVSLFSGCGGSDAGVLNAGFNVLMANDILPYARDVYLENHP
                     ETDYILGDISGLQSFPSAELLIGCYPCQGFSQGGARKADRKINTLYLEFARALSKIKP
                     KAFIVENVSGMVRRNFEHLLKDQFKVFEEAGYTVSSQILNASHYGVSQDRKRIFIVGI
                     RKDYGITYKFPKPTHGDGLTPYSTIRDAIGHMPVWPVGEFYDADFHWYYLSRNRRQDW
                     DQISKTIVANPRHMPLHPISPTLEKLGPDKWQFTSDAPARRFSYREAAILQGFGDLIF
                     PETERASINMKYTVVGNAVPPPLFEAVARNLPDLWD"
     gene            complement(26854..27060)
                     /locus_tag="PN977_000907"
     CDS             complement(26854..27060)
                     /locus_tag="PN977_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000554768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6040970.1"
                     /translation="MHRIPGEIPHHKTKNIKLMAIVQRLQRIMVNENLTPDELVGCAE
                     IVRDNYGRFNHIGQSRVAPPQRRR"
     gene            complement(27060..27512)
                     /locus_tag="PN977_000908"
     CDS             complement(27060..27512)
                     /locus_tag="PN977_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001364189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3850 domain-containing protein"
                     /protein_id="HDI6040971.1"
                     /translation="MAKIHEVKLHAKYFDLVLEGKKRAEFRKNDRNYERGDTLILYEW
                     VQGVYTGRNVEARITDVTDLSDWLEDYVLLSIELLNTGAYEIVNWKELSERGLVFRIN
                     HEIMHQLGLAVMYEPETGMSGGAMVATDGAWNYSDEQMERAQQNGWLG"
     gene            complement(27512..29797)
                     /locus_tag="PN977_000909"
     CDS             complement(27512..29797)
                     /locus_tag="PN977_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016235174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication endonuclease"
                     /protein_id="HDI6040972.1"
                     /translation="MAVKASGRFVPPSAFAAGTGKMFTGAYAWNAPREAVGRERPLTR
                     DEMRQVQGVLSTINRLPYFLRSLFTSRYDYIRRNKSPVHGFYFLTSTFQRRLWPRIER
                     VNQRHEMNTDASLLFLAERDHYARLPGMNDKELKKFAARISSQLFIMYEELCDAWVDA
                     HGEKESLFTDEAQAHLYGHVAGAARAFNISPLYWKKYRKGQMTTRQAYSAIARLFNDE
                     WWTHQLKGQRMRWHEALLIAVGEVNKDRSPYASKHAIRDVRARRQANLEFLKSCDLEN
                     RETGERIDLISKVMGSISNPEIRRMELMNTIAGIERYAAAEGDVGMFITLTAPSKYHP
                     TRQVGKGESKTVQLNHGWNDEAFNPKDAQRYLCRIWSLMRTAFKDNDLQAYGLRVVEP
                     HHDGTPHWHMMLFCNPRQRNQIIEIMRRYALKEDGDERGAARNRFQAKHLNRGGAAGY
                     IAKYISKNIDGYALDGQLDNDTGRPLKDTAAAVTAWASTWRIPQFKTVGLPTMGAYRE
                     LRKLPRGVSIADEFDERVEAARAAADSGDFALYISAQGGANVPRDCQTVRVARSPSSD
                     VNEYEEEVERVVGIYAPHLGARHIHITRTTDWRIVPKVPVVEPLTLKSGIAAPRSPVN
                     NCGKLTGSDTSLPAPTPYEHAAAVLNLVDDGVIEWNEPEVVRALRGALKHELRTPNRQ
                     QRNGSPLKPHEIAPSARLTRSERTQITRIRVDLAQNGIRPQRWELEALARGATVNYDG
                     KKFTYPVADEWPGFSTVMEWT"
     gene            complement(29787..30062)
                     /locus_tag="PN977_000910"
     CDS             complement(29787..30062)
                     /locus_tag="PN977_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000027668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5405 family protein"
                     /protein_id="HDI6040973.1"
                     /translation="MSIRIEIGERYVVTSDSFQFILHEKKRAESGKNAGQEWLAVVGY
                     YPKLSQLVSGLMHHDILTGSAKSFADLNAQVEQLSRRCSEAFGSYGR"
     gene            complement(30059..30283)
                     /locus_tag="PN977_000911"
     CDS             complement(30059..30283)
                     /locus_tag="PN977_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016242005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HDI6040974.1"
                     /translation="MPDNVDFIQEQQAELLERQINAARVKHCGASALVCEECDAPIPA
                     ARRAAYPSATRCVSCQSVFEAKNKHYRRTA"
     gene            complement(30283..30585)
                     /locus_tag="PN977_000912"
     CDS             complement(30283..30585)
                     /locus_tag="PN977_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001277953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5405 family protein"
                     /protein_id="HDI6040975.1"
                     /translation="MSCSRSVVLLNNALKIAVMKNGDLSLIQLGLDKEKREITESVIA
                     IYQNELNLLSDVVNLLVKRAVFHKQISSVDELTKLTTEIASYCADEFKNLNDKRNW"
     gene            complement(30585..30809)
                     /locus_tag="PN977_000913"
     CDS             complement(30585..30809)
                     /locus_tag="PN977_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016236090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2732 family protein"
                     /protein_id="HDI6040976.1"
                     /translation="MHTVSENQCGKYALLLQQARTEAQADAATRFSSHLDAMIRHITK
                     AELSRVEIVELLSQESEKFHNIGLSRGEVL"
     gene            complement(30873..31373)
                     /locus_tag="PN977_000914"
     CDS             complement(30873..31373)
                     /locus_tag="PN977_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017441883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein B"
                     /protein_id="HDI6040977.1"
                     /translation="MTVMTLNLVEKQPAAMRRIIGKHLAVPRWQDTCDYYNQMMERER
                     LTVCFHAQLKQRHATMRFEEMNDVERERLVCAIDELRGAFSKRRQVGASEYAYISFLT
                     VSQRRTLFMHARLTEKEFNQPYWRINEESCYWRDALFRALRELFSLFEYAPTILTSVK
                     PEQYLH"
     gene            complement(31370..31567)
                     /locus_tag="PN977_000915"
     CDS             complement(31370..31567)
                     /locus_tag="PN977_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001350035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6040978.1"
                     /translation="MAFVDGVVIMSEPRCIAQLLRNESPRAIDFTITHGKGRKGIIIR
                     TKKQSPLKKALTFLKSRRGWK"
     gene            complement(31543..31815)
                     /locus_tag="PN977_000916"
     CDS             complement(31543..31815)
                     /locus_tag="PN977_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001583792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6040979.1"
                     /translation="MEVNDYVIQYPIDAVHTVKFAELLGKPETAVVKMVKENKLPVIE
                     LRDPSKPNARVGEKWVFIPEFNRAVREAFYNRPVEQRDAWLLWMGL"
     gene            31968..32261
                     /locus_tag="PN977_000917"
     CDS             31968..32261
                     /locus_tag="PN977_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001192859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDI6040980.1"
                     /translation="MQTFEKLKAIRKAEGLTQAKFSEISGIALGTVKNYESGHKDPGL
                     SIVMRVTNTPLFKKYTLWLMTGDTSPQAGQIAPALAHIGQKPTESDHSEKQTG"
     gene            32331..33311
                     /locus_tag="PN977_000918"
     CDS             32331..33311
                     /locus_tag="PN977_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000985242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDI6040981.1"
                     /translation="MSIKKLDDGRYEVDIRPRGRDGKRIRRKFERKAEAVAFERYTIA
                     YASQKEWAGQRADRRTLSELLNIWWKYHGQNHEHGTKEFNHLLKTISGIGDIPVSRMS
                     KRALMDYRSMRLRDGISAATINRDMYRLSGMFTKLIQLDEFSGQHPIHGLPPLAEANP
                     EMTFLEKAEIEKLLNVLDGDDLLVALLCLSTGGRWTEVATLKPAQITNCRVTFLKTKN
                     GKKRTVPISEELEKKVKEEASAKLFKVDYEKFCGILRRVKPDIPPNQATHILRHTFAS
                     HFMMNGGNIIALQQILGHASIQQTMAYAHLAPDYLQNAVALNPLKGGVTL"
     gene            complement(33497..33997)
                     /gene="cpxP"
                     /locus_tag="PN977_000919"
     CDS             complement(33497..33997)
                     /gene="cpxP"
                     /locus_tag="PN977_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell-envelope stress modulator CpxP"
                     /protein_id="HDI6040982.1"
                     /translation="MRIVTAAVMASTLAVSSLSHAAEVGSGDNWHPGEELTQRSTQSH
                     MFDGISLTEHQRQQMRDLMQQARHEQPPVNVSELETMHRLVTAENFDENAVRAQAEKM
                     ANEQIARQVEMAKVRNQMYRLLTPEQQAVLNEKHQQRMEQLRDVTQWQKSSSLKLLSS
                     SNSRSQ"
     gene            34147..34845
                     /gene="cpxR"
                     /locus_tag="PN977_000920"
     CDS             34147..34845
                     /gene="cpxR"
                     /locus_tag="PN977_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response regulator transcription
                     factor CpxR"
                     /protein_id="HDI6040983.1"
                     /translation="MNKILLVDDDRELTSLLKELLEMEGFNVIVAHDGEQALDLLDDS
                     IDLLLLDVMMPKKNGIDTLKALRQTHQTPVIMLTARGSELDRVLGLELGADDYLPKPF
                     NDRELVARIRAILRRSHWSEQQQNNDNGSPTLEVDALVLNPGRQEASFDGQTLELTGT
                     EFTLLYLLAQHLGQVVSREHLSQEVLGKRLTPFDRAIDMHISNLRRKLPDRKDGHPWF
                     KTLRGRGYLMVSAS"
     gene            34842..36215
                     /gene="cpxA"
                     /locus_tag="PN977_000921"
     CDS             34842..36215
                     /gene="cpxA"
                     /locus_tag="PN977_000921"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress sensor histidine kinase CpxA"
                     /protein_id="HDI6040984.1"
                     /translation="MIGSLTARIFAIFWLTLALVLMLVLMLPKLDSRQMTELLDSEQR
                     QGLMIEQHVEAELANDPPNDLMWWRRLFRAIDKWAPPGQRLLLVTTEGRVIGAERSEM
                     QIIRNFIGQADNADHPQKKKYGRVELVGPFSVRDGEDNYQLYLIRPASSSQSDFINLL
                     FDRPLLLLIVTMLVSTPLLLWLAWSLAKPARKLKNAADEVAQGNLRQHPELEAGPQEF
                     LAAGASFNQMVTALERMMTSQQRLLSDISHELRTPLTRLQLGTALLRRRSGESKELER
                     IETEAQRLDSMINDLLVMSRNQQKNALVSETIKANQLWSEVLDNAAFEAEQMGKSLTV
                     NFPPGPWPLYGNPNALESALENIVRNALRYSHTKIEVGFAVDKDGITITVDDDGPGVS
                     PEDREQIFRPFYRTDEARDRESGGTGLGLAIVETAIQQHRGWVKAEDSPLGGLRLVIW
                     LPLYKRS"
     gene            complement(36320..36994)
                     /gene="yiiM"
                     /locus_tag="PN977_000922"
     CDS             complement(36320..36994)
                     /gene="yiiM"
                     /locus_tag="PN977_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418346.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-N-hydroxylaminopurine resistance protein"
                     /protein_id="HDI6040985.1"
                     /translation="MRYPVDVYTGKIQAYPEGKPSAIAKIQVDGELMLTELGLEGDEQ
                     AEKMVHGGPDRALCHYPREHYLYWAREFPEQAELFVAPAFGENLSTDGLTESNVYMGD
                     IFRWGEALIQVSQPRSPCYKLNYHFDISDIAQLMQNTGKVGWLYSVIAPGLVSADAPL
                     ELVSRVSDVTVQEAAAIAWHMPFDDDQYHRLLSAAGLSKSWTRTMQKRRLSGKIEDFS
                     RRLWGK"
     gene            complement(37143..38126)
                     /gene="kdgT"
                     /locus_tag="PN977_000923"
     CDS             complement(37143..38126)
                     /gene="kdgT"
                     /locus_tag="PN977_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxygluconate transporter"
                     /protein_id="HDI6040986.1"
                     /translation="MQIKRSIEKIPGGMMLVPLFLGALCHTFSPGAGKYFGSFTNGMI
                     TGTVPILAVWFFCMGASIKLSATGTVLRKSGTLVVTKIAVAWVVAAIASRIIPEHGVE
                     VGFFAGLSTLALVAAMDMTNGGLYASIMQQYGTKEEAGAFVLMSLESGPLMTMIILGT
                     AGIASFEPHVFVGAVLPFLVGFALGNLDPELREFFSKAVQTLIPFFAFALGNTIDLTV
                     IAQTGLLGILLGVAVIIVTGIPLIIADKLIGGGDGTAGIAASSSAGAAVATPVLIAEM
                     VPAFKPMAPAATSLVATAVIVTSILVPILTSIWSRKVKARAAKIEILGTVK"
     gene            complement(38386..39006)
                     /gene="sodA"
                     /locus_tag="PN977_000924"
     CDS             complement(38386..39006)
                     /gene="sodA"
                     /locus_tag="PN977_000924"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010345169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Mn]"
                     /protein_id="HDI6040987.1"
                     /translation="MSYTLPSLPYAYDALEPHFDKQTMEIHHTKHHQTYVNNANAALE
                     SLPEFANLPVEELITKLDQLPADKKTVLRNNAGGHANHSLFWKGLKKGTTLQGDLKAA
                     IERDFGSVDNFKAEFEKAAASRFGSGWAWLVLKGDKLAVVSTANQDSPLMGEAISGAS
                     GFPIVGLDVWEHAYYLKFQNRRPDYIKEFWNVVNWDEAAARFAAKK"
     gene            39291..40325
                     /gene="rhaT"
                     /locus_tag="PN977_000925"
     CDS             39291..40325
                     /gene="rhaT"
                     /locus_tag="PN977_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose/proton symporter RhaT"
                     /protein_id="HDI6040988.1"
                     /translation="MSNAITMGIFWHLIGAASAACFYAPFKKVKKWSWETMWSVGGIV
                     SWIILPWAISALLLPNFWAYYSSFSLSTLLPVFLFGAMWGIGNINYGLTMRYLGMSMG
                     IGIAIGITLIVGTLMTPIINGNFDVLINTEGGRMTLLGVLVALIGVGIVTRAGQLKER
                     KMGIKAEEFNLKKGLVLAVMCGIFSAGMSFAMNAAKPMHEAAAALGVDPLYVALPSYV
                     VIMGGGAIINLGFCFIRLAKVKDLSLKADFSLAKPLIIHNVLLSALGGLMWYLQFFFY
                     AWGHARIPAQYDYISWMLHMSFYVLCGGIVGLVLKEWNNAGRRPVTVLSLGCVVIIVA
                     ANIVGMGMAN"
     gene            complement(40322..41188)
                     /gene="rhaR"
                     /locus_tag="PN977_000926"
     CDS             complement(40322..41188)
                     /gene="rhaR"
                     /locus_tag="PN977_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaR"
                     /protein_id="HDI6040989.1"
                     /translation="MTEVATVAHQLKLLKDDFFASDQQAVAVADRYPQDVFAEHTHDF
                     CELVIVWRGNGLHVLNDRPYRITRGDLFYIHADDKHSYASVNDLVLQNIIYCPERLKL
                     NLDWQGAIPGFSASAGQPHWRLGSMGMAQARQIIGQLEHESSQHVPFANEMAELLFGQ
                     LVMSLNRHRYTSDSLPPTSSETLLDKLITRLAASLKSPFALDEFCDEASCSERVLRQQ
                     FRQQTGMTINQYLRQVRVCHAQYLLQHSRLLISDISTECGFEDSNYFSVVFTRETGMT
                     PSQWRHLNSQKD"
     gene            complement(41244..42080)
                     /gene="rhaS"
                     /locus_tag="PN977_000927"
     CDS             complement(41244..42080)
                     /gene="rhaS"
                     /locus_tag="PN977_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaS"
                     /protein_id="HDI6040990.1"
                     /translation="MTVLHSVDFFPSGNASVAIEPRLPQADFPEHHHDFHEIVIVEHG
                     TGIHVFNGQPYTITGGTVCFVRDHDRHLYEHTDNLCLTNVLYRSPDRFQFLAGLNQLL
                     PQEQDGQYPSHWRVNHSVLQQVRQLVAQMEQQEEENDLPSTASREILFMQLLLLLRKS
                     SLQENLENSASRLNLLLAWLEDHFADEVNWDAVADQFSLSLRTLHRQLKQQTGLTPQR
                     YLNRLRLMKARHLLRHSEASVTDIAYRCGFSDSNHFSTLFRREFNWSPRDIRQGRDGF
                     LQ"
     gene            42368..43837
                     /gene="rhaB"
                     /locus_tag="PN977_000928"
     CDS             42368..43837
                     /gene="rhaB"
                     /locus_tag="PN977_000928"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HDI6040991.1"
                     /translation="MTFRNCVAVDLGASSGRVMLARYERECRSLTLREIYRFNNGLHS
                     QNGYVTWDVDSLESAIRLGLNKVCEEGIRIDSIGIDTWGVDFVLLDLQGKRVGLPVAY
                     RDSRTNGLMTQAQQQLGKRDIYQRSGIQFLPFNTLYQLRALTEQQPELIPHIAHALLM
                     PDYFSYRLTGKMNWEYTNATTTQLVNINSDDWDESLLAWSGANKAWFGRPTHPGNVIG
                     HWICPQGNEIPVVAVASHDTASAVIASPLNGSRAAYLSSGTWSLMGFESQTPFTNDTA
                     LAANITNEGGAEGRYRVLKNIMGLWLLQRVLQERQINDLPALIAATQALPACRFTINP
                     NDDRFINPDEMCSEIQAACRETAQPIPESDAELARCIVDSLALLYADVLHELAQLRGE
                     DFSQLHIVGGGCQNALLNQLCADACGIRVIAGPVEASTLGNIGIQLMTLDELNNVDDF
                     RQVVSTTANLTTFTPNPDSEIAHYVAQIHSTRQTKELCA"
     gene            43834..45093
                     /gene="rhaA"
                     /locus_tag="PN977_000929"
     CDS             43834..45093
                     /gene="rhaA"
                     /locus_tag="PN977_000929"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HDI6040992.1"
                     /translation="MTTQLEQAWELAKQRFAAVGIDVEEALRQLDRLPVSMHCWQGDD
                     VSGFENPEGSLTGGIQATGNYPGKARNASELRADLEQAMRLIPGPKRLNLHAIYLESD
                     TPVSRDQIKPEHFKNWVEWAKANQLGLDFNPSCFSHPLSADGFTLSHADDSIRQFWID
                     HCKASRRVSAYFGEQLGTPSVMNIWIPDGMKDITVDRLAPRQRLLAALDEVISEKLDP
                     AHHIDAVESKLFGIGAESYTVGSNEFYMGYATSRQTALCLDAGHFHPTEVISDKISAA
                     MLYVPQLLLHVSRPVRWDSDHVVLLDDETQAIASEIVRHDLFDRVHIGLDFFDASINR
                     IAAWVIGTRNMKKALLRALLEPTTELRKLEAAGDYTARLALLEEQKSLPWQAVWEMYC
                     QRHDTPVGSEWLESVRAYEKAILSQRG"
     gene            45176..46000
                     /gene="rhaD"
                     /locus_tag="PN977_000930"
     CDS             45176..46000
                     /gene="rhaD"
                     /locus_tag="PN977_000930"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HDI6040993.1"
                     /translation="MQNITQSWFVQGMIKATTDAWLKGWDERNGGNLTLRLDDADIAP
                     YHDNFHAQPRYIPLSQPMPLLANTPFIVTGSGKFFRNVQLDPAANLGVVKVDSDGAGY
                     HILWGLTNEAVPTSELPAHFLSHCERIKATNGKDRVIMHCHATNLIALTYVLENDTAV
                     FTRQLWEGSTECLVVFPDGVGILPWMVPGTDEIGQATAQEMQKHSLVLWPFHGVFGSG
                     PTLDETFGLIDTAEKSAQVLVKVYSMGGMKQTISREELIALGQRFGVTPLASALAL"
     gene            46010..46324
                     /gene="rhaM"
                     /locus_tag="PN977_000931"
     CDS             46010..46324
                     /gene="rhaM"
                     /locus_tag="PN977_000931"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HDI6040994.1"
                     /translation="MIRKAFVMQVNPDAHEEYQRRHNPIWPELEAVLKSHGAHNYAIY
                     LDKARNLLFATVEIESEERWNAVASTDVCQRWWKYMTDVMPANPDNSPVSSELQEVFY
                     LP"
     gene            complement(46365..47759)
                     /locus_tag="PN977_000932"
     CDS             complement(46365..47759)
                     /locus_tag="PN977_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate porin"
                     /protein_id="HDI6040995.1"
                     /translation="MKKSTLSLAIGLLLACSTGMAKTQHLTLEQRMALLEERLEAAEM
                     RAAKAESQVKQLQTQQAAEIREIKAAQGNTPVNGQATAESAKKNSTSPNLLLSGYGDL
                     KIYGDVEFNMDAESNHGLLAMTNADVNSDPTNEQWNLNGRILLGFDGMRKLDNGYFAG
                     FSAQPLGDMHGSVNIDDAVFFFGKENDWKVKVGRFEAYDMFPLNQDTFVEHSGNTAND
                     LYDDGSGYIYMMKEGRGRSNAGGNFLVSKQLDNWYFELNTLLEDGTSLYNDGNYHGRD
                     MEQQKNVAYLRPVIAWSPTEEFTVSAAMEANVVNNAYGYTDSKGNFVDQSDRTGYGMS
                     MTWNGLKTDPENGVVVNLNTAYLDANNEKDFTAGINALWKRFELGYIYAHNKIDEFSG
                     VVCDNDCWIDDEGTYTIHTIHASYQFANVMDMENFNIYLGTYYSILDSDGDKKHGDDT
                     DDRYGARVRFKYFF"
     gene            48236..48682
                     /locus_tag="PN977_000933"
     CDS             48236..48682
                     /locus_tag="PN977_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HDI6040996.1"
                     /translation="MAALTASCIDLNIQGNGAYSVLKQLATMALQNGFITDSHQFLQT
                     LLLREKMHSTGFGSGIAVPHGKSACVKQPFVLFARKTQAIDWQASDGEDVNCWICLGV
                     PQSGEEEQVKIIGTLCRKIIHQDFINQLRQGDADQVLALLNQTLSS"
     gene            48693..50144
                     /locus_tag="PN977_000934"
     CDS             48693..50144
                     /locus_tag="PN977_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIBC"
                     /protein_id="HDI6040997.1"
                     /translation="MESSLRIVAITNCPAGIAHTYMVAEALEQKARSLGHTIKVETQG
                     SSGIENRLSSEEIAAADYVILATGRGLSGDDRARFAGKKVYEIAISQALKNIDQIFSE
                     LPTNSQLFAADSGVKLGKQEVQSGSVMSHLMAGVSAALPFVIGGGILVALANMLVQFG
                     LPYTDMSKGAPSFTWVVESIGYLGFTFMIPIMGAYIASSIADKPAFAPAFLVCYLAND
                     KALLGTQSGAGFLGAVVLGLAIGYFVFWFRKVRLGKALQPLLGSMLIPFVTLLVFGVL
                     TYYVIGPVMSDLMGGLLHFLNTIPPSMKFAAAFLVGAMLAFDMGGPINKTAWFFCFSL
                     LEKHIYDWYAIVGVVALMPPVAAGLATFIAPKLFTQQEKEAASSAIVVGATVATEPAI
                     PYALAAPLPMITANTLAGGITGVLVIAFGIKRLAPGLGIFDPLIGLMSPVGSFYLVLA
                     IGLALNISFIIVLKGLWLRRKAKAAQQELVHEH"
     gene            50134..51204
                     /locus_tag="PN977_000935"
     CDS             50134..51204
                     /locus_tag="PN977_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HDI6040998.1"
                     /translation="MNIELLQQLCEASAVSGDEQEVRDILINTLEPCVNEITFDGLGS
                     FVARKGNKGPKVAVVGHMDEVGFMVTHIDKSGFLRFTTIGGWWNQSMLNHRVTIRTHK
                     GVKIPGVIGSVAPHALTEKQKQQPLSFDEMFIDIGANSREEVEKRGVEIGNFISPEAN
                     FACWGEDKVVGKALDNRIGCAMMAELLQTVNNPEITLYGVGSVEEEVGLRGAQTSAEH
                     IKPDVVIVLDTAVAGDVPGIDNIKYPLKLGQGPGLMLFDKRYFPNQKLVAALKNCAAH
                     NDLPLQCSTMKTGATDGGRYNVMGGGRPVVALCLPTRYLHANSGMISKADYDALLTLI
                     RDFLTTLTAEKVNAFSQFRQVD"
     gene            51204..52952
                     /locus_tag="PN977_000936"
     CDS             51204..52952
                     /locus_tag="PN977_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative frv operon regulatory protein"
                     /protein_id="HDI6040999.1"
                     /translation="MLNERQLKIVDLLEQQPRTPGELAQQTGVSGRTILRDIDYLNFT
                     LNGKARISASGNAGYQLEIFERRSFFQLLQKHDNDDRLLALLLLNTFTPRAQLASALN
                     LPETWVAERLPRLKQRYERGFCLASRPGLGHFIDETEEKRIILLANLLRKDPFLIPLS
                     GVTRDNFQQLTTACEKQRRWPLMQGDYLSSLILAIYALRNQLTDEWPQYPGDEIKQIV
                     EQSGLFLGDNAVRTLTGLIEKQHQQAQVISADHVLGLLQRVPGIASLNIIDTQLVENI
                     TGHLLRCLAAPVWIAEHRQSSMNNLKAAWPAAFDMSLHFITLLREQLDIPLFDSDLIG
                     LYFACALERHQNERQPIILLSDQNAIATINQLAIERDVLHCRVIIARSLSELVAIREE
                     IEPLLIINNSHYLLDDAVNNYITVKNIITAAGIEQIKHFLATAFIRQQPERFFSAPGS
                     FHYSNVRGESWQHITRQICAQLVAQHHITADEAQRIIAREGEGENLIVNRLAIPHCWS
                     EQERRFRGFFITLAQPVEVNNEVVNHVLIACAAADARHELKIFSYLASVLCQHPAEVI
                     AGLTGYEAFMELLHKG"
     gene            complement(53008..54063)
                     /locus_tag="PN977_000937"
     CDS             complement(53008..54063)
                     /locus_tag="PN977_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiG family protein"
                     /protein_id="HDI6041000.1"
                     /translation="MKRNLLSSAIIVAIMALGLTGCDDKKAETETPPPANSQPAAPAP
                     EAKPAEAPVAKAEVKPETPAQPVVDEQAVFDEKMDVYIKCYNKLQIPVQRSLARYADW
                     LKDFKQGPTGKESTVYGIYGFSESSLAECEKGVKSAVALTPALQPIDGVAVSYIDAAV
                     ALGNTINEMDKYYTQENYKDDAFAKGKTLHQTFLKNLEAFEPVAESYHAAIQEINDKR
                     QLAELKNIEEREGKTFHYYSLAVMISAKQINNLISQEKFDVDAAMKKVSELETLVAQA
                     KEADKGGMNFSFINSADQYQLEAKKYVRRVRDKVPYSDWDKEQLQDANTSWMVDDSFP
                     RALREYNEMVDDYNSLR"
     gene            complement(54258..56564)
                     /locus_tag="PN977_000938"
     CDS             complement(54258..56564)
                     /locus_tag="PN977_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001591275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6041001.1"
                     /translation="MDIWEWYHKYTKELRDAGQEYIPQLIDDYSEGVMHLQMEKCDSL
                     LPEVKALTQVAKNPWLEIFIGHWEMRHRLTNYEEGEKVLPDVVALFEKAHREETKDCP
                     QSICVTQDLSACYENIDAPGWANERMAVCEETIARIDPTWSCFQCLSTEYASALIDSK
                     RIEEALAYLDRQIDAVKAAGEDDIDALLYTRADILIELQRYDEARILIDELTEANDYW
                     GRDRISNHLKKLYIMALCGQDEEVWEELPRWSDLTPHSWGSWLKIITVLLPRHPQRNN
                     WQLARIIQKMLEHRSQAGAHRKVVDLAKLQITLALERESVWVAKIAMKLAQRHMPKLR
                     VLLGVDREFLELSEKIAAKEAELLARPVVEVPENSDEETQPDPEKDVETFRRAYLNAP
                     DDAELLGKLVNALNACNAIDESIELLQRYVESHIQEDNGLCFDLLDLLLHRDDLQTIE
                     HLAKQYEQAGLLHMALWFRARVINQQEDWSELESIGHQLLQNEKGREWIGVYRMCSWA
                     AQKQEAWDRQLDYLQQLQQLMISREEDIRNIQWDIMATASILQQWDLVRAIAAELEIE
                     LTEGEGFIDEKWGLLNIRFRENYEYRYYYAQRIGPVTARIIEPTYRTDYPQHVDEVVV
                     FDAEILNTQPEDEEERKNFTPLYACLQTIIPTEYGESWFCEGVMPGEEQFNAFREALR
                     ERNWYYWSNSNEDYTLTDSQQGEDAQPLPAFYFVISAPRSVSKGDIDKTLHELTADWE
                     HPLCWWRLAKEAGANEEKHAAISERYGM"
     gene            complement(56566..58401)
                     /locus_tag="PN977_000939"
     CDS             complement(56566..58401)
                     /locus_tag="PN977_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000105780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDI6041002.1"
                     /translation="MSTLQLPGSSYSTEVNLNGLIEVLSKHLYSTPVVAVRELVQNGH
                     DAIVRRRIEQPDAPKDNAIRVVADVAKSTITISDTGAGLTESEIHGFLATVGVGYTRM
                     LRQQDDNTGLIGMFGLGFLSAFVLAKEVTVLTTSWQTPDQSWKYHSTDGQKYTVTPHQ
                     SSETGTQVILTLKEEYSHLASNNLLNRVLSRYCILLHEPVYVGDASEPVNKLQPPWRE
                     VAPEGVTMHRALVQRKNLAFAAQFESSFEPICTIPVVPVGMSDAVGILWIQDGATYGT
                     SDNRNLSLFLRGMLLDDEARELLPPWAGFIGGVIESSKLTPTASREDLQRDETWVAVQ
                     EALKEALISGLSDLAQNQPEIWRRVLMRHNEALLGAALCDDRLFDLLKDRLQVPTSKG
                     ALLAKDLRVNNSIHILLSRDGGFEEMLFHILQRPVARGDRYAVVPFLRRWALLYHCRI
                     VEVGTQTGNEQLFSLAELPEEQVAYLEEHLCDGEQLIISRFEPAVLPLVVTPDREAEL
                     KQILEQDDADKRISTAALMLARQFTSQIQKTKTSSLYINLNNPCIMQLVTALQHQQQP
                     AAALRLLKSLKVILCSSGNKEQQWDLHQALEDFTQVIPVLINQGK"
     gene            complement(58711..59547)
                     /gene="fdhD"
                     /locus_tag="PN977_000940"
     CDS             complement(58711..59547)
                     /gene="fdhD"
                     /locus_tag="PN977_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000753607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HDI6041003.1"
                     /translation="MKKTQQKEIENVTSVTGVRQIELWRRDDLQHPRLDEVAEEVPVA
                     LVYNGISHVVMMASPKDLEYFALGFSLSEGIIESPRDIFGMDVVPSCNGLEVQIELSS
                     RRFMGLKERRRALAGRTGCGVCGVEQLNDIGKPVQPLPFTQTFDLNKLDDALRHLNDF
                     QPVGQLTGCTHAAAWMLPSGELVGGHEDVGRHVALDKLLGRRSQEGESWQQGAVLVSS
                     RASYEMVQKSAMCGVEILFAVSAATTLAVEVAERCNLTLVGFCKPGRATVYTHPQRLI
                     HN"
     gene            59741..>60321
                     /locus_tag="PN977_000941"
     CDS             59741..>60321
                     /locus_tag="PN977_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HDI6041004.1"
                     /translation="MQVSRRQFFKICAGGMAGTTAAALGFAPSVALAETRQYKLLRTR
                     ETRNTCTYCSVGCGLLMYSLGDGAKNAKASIFHIEGDPDHPVNRGALCPKGAGLVDFI
                     HSESRLKFPEYRAPGSDKWQQISWEEAFDRIAKLMKEDRDANYIAQNAEGVTVNRWLS
                     TGMLCASASSNETGYLTQKFSRALGMLAVDNQAR"
BASE COUNT        14554 a        15942 c        14776 g        15049 t
ORIGIN      
        1 aactgagggg ctatttttgt gatcacatcc acataatttg ctgccctgac ggcaacgggt
       61 gcggcctcac ggcgtggact tctcccggct tcacgatgta tctctgtacc gactcataag
      121 tgatgaacgt ggcgctgcaa ttcacgttct ggcactggtg ataacgctct tttgtcgtgt
      181 cagtgatata gcggcttgta cgcgcatgtg cggcatgctg gcataaagga caatgaaaca
      241 tcgcgagcac ctcttccggt tttgttgata gtgccatttt agttaaatta tcattataaa
      301 acaaaaagat aaacaaaaga catcactcat aatcttctgt ttcgtactcc acatcagaaa
      361 gcctgacctc aagctctaag gacgtcgtga agccgctatt attcagaaaa tgtgtcacct
      421 tagtgactgt ccagtcctgc tcgtctatga cgcgcttaaa gcctgacact ctgaccggtg
      481 tttccgtgta aatatctgca cgaccggtag ccaggatgat ggagaactcc gcaacgcccc
      541 gttgcagttt atcccacttc gcctgagcag cacgcatggc ctgtgctttc gtggcatata
      601 ccgtggtcag ggcaaaaacg ttgtcagcct caccggccat gtattcacct tcgcgcgctt
      661 ccggtacttt tggcgctttc ttctgcgtga ctggtttcgc tttcgggtgc tccagtgcgc
      721 gcaggtgttt ttctttcttt ttgcgtttca gttttacctt ctgcttttgc ggcttcgggt
      781 ctttggtgtg aagccacttt gccgttacgc cggtataggc tccacggtca gcaatcgcaa
      841 aatgatgacg gtcgccgtcg ctgcgggtga tggtaatctg cgggattttt ttaccgctgg
      901 ccgtcacccc ctgccccgct ttgagaaaca acagttttcc catttttacc gacacctcac
      961 cgccgttgcg ttcagcaagg cgggtcagga attttgcatc agactcctgc gactggtcga
     1021 tgtgcgggat tttaattccg gccagtgacg gagcgacact ggcttccagc ctgttacggg
     1081 aggctatcgc ctcaacaatc gcaccgagcg tggtgtcatg ccaggagcct tcacggcggg
     1141 aattgagcgt gccacgaaaa tctgcactcc gggcccggat ggcgaccaca tccggcgtgc
     1201 cccggtgttc tacctcatca acggtgaatt tccctttgca taccagggca aaacctttcc
     1261 agccgatata caccgtcaga acagcgccac gaaccggcag ttcgacctgc ccgtcggcat
     1321 cgttcagttc aatatcaagc tgatcagcct caaagccccg gttatccgtc agggtcatgc
     1381 tcatcagacg gtcgctgata ttgccggtaa tatccctgct gtcgagcatc agcatgtaat
     1441 ccggcgtcag cgtactgcct gcatcaaatg tcagcgcatc cagcattatt ccgcccccgt
     1501 catccccgtg aatctggtcg ccagactacc ggccttaccg atgagcgact ccgcctgttt
     1561 accgatatcg ccataaagcg cggccagtga ttcatcaacg cgggtgagcg acagcgtaaa
     1621 atcaattttt cggggtgtgc cgtctgcaaa gaaaatactc cctgtttcac tcaccttgct
     1681 gatgacatac atgccgtaaa tcatgcctgt gccatccagc aacggccacg cccggccttc
     1741 ctctgccatc agcctgagcg tggtcatcgt cagcttgccg ccggtcagtt cgggataaag
     1801 cacgccggca agcgtaatgt tttcctcacc cacaccgaga aactgaaagg catcccgttt
     1861 accgatacgg gaatttgacg gccagcgata atctgattcg cgctgcatgg tctggtgtgg
     1921 cagcgtctgg cgcataaaaa caaacatacc taacgcgagc atcattttta gtcacctcct
     1981 taaccgtcat gcatcatgct ggcacgggcg cgcacacgtt tatcccgctc gtatttttcg
     2041 agcgcatcct gtaactggcg gtcgagctgt gtccccggcg cagtaccacc cgtcaggctg
     2101 atgtgatatt cgtttttact ctggtccaca taagagcggc cagccggtgc cgtgaccggc
     2161 tgataagcct gatagcctgc ataagagctg gtcgccggaa tataaccacc gctgccatac
     2221 gtggcggctt gagttctggc ggcggtctgg tcaagtgtgt ctgactcttt gttgataaca
     2281 ccgagttttt ccagtaccca gtcaataccg ctgcgcagtt tgttgaacgc attaagcggc
     2341 agcatcagcg cgtcagccag tgcctgcccg aacatgaccc ccgtgtcacg gcaactgttc
     2401 agggagtcct gagtggcttt gaccggggca atcaggtttt taaaccactg ccacgcggcc
     2461 tgtaactttt cacccagcca gtcaaacacc ggcttaagtg gcgtgaacag ttcccccacc
     2521 ggcgcaaatg ccactttcag cccttcaacc acaccgccaa agaatgcgct gacaggctcc
     2581 cagtatttac ggataagcaa cgccccggcg acaattgcgg ccaccacggc cacaaccggc
     2641 cagctaataa ccccgatggc ggtcataaca gcactgccaa ccgtcgtgaa gactgccccc
     2701 attgcgcctg ctgccgcgat gatggcattg atgccggtga taaccggcca ggctacgagg
     2761 ccaatggcac cgatgatgcc agtcagcgcc agcgcgccac cggcaatgat gccgatggtt
     2821 gacgccagtg atttgttttt ctggatccag ccgtcgagtt ttaacacata ctttgtggtc
     2881 gtctgcgtga gcttacgcag cgcgccttcc tgctggtcaa acaggtcagt ccccactgcc
     2941 tcataagcgg actgaaactc tttaaagtca ccgcctaggt tgtcctgcat gatatttacc
     3001 agctcggcgg tcttcccgtc tgaggattta aacgcagcgg tcagtttgtc cagctttccg
     3061 gttgaggcgg cagtcatcag cacggcggcg gctgagctgg cctcctcccc gaaaatagtt
     3121 ttcatgtatt cagcctgctg ggcagtaccg agccggtttt tctcaaaact ggcctgcatt
     3181 tctttcagaa tggtaaatac tggtcgggtg tttcccttgc tgtctgaggt tttcactcca
     3241 agctctttga gtgcatccca tgcttttccc gtcggtgcct gcaggcggct taacacggca
     3301 cggcttcccg tccccgccat tgagcctgtg atttttgcat catgcagcgc cccgaccatt
     3361 gcggcggttt cttcaatgct gacaccggca ttttttgcca caggtgcggc ataggtcagc
     3421 gcatcgctca tgccgtcaaa atcggcggcg gttttgttca tcgtcatgga gagaacatcc
     3481 ccgatatgag cgaccttatc gtttgaaagc tgaaaggcgg atttcatccc catcaacagg
     3541 gcggcgtttt cttccatcgt gcggcggttc gccagcgcca tattcagcgt gaccggcgtt
     3601 gccgcctgaa tggcatcaac atccccaccc gctttcgcga tgattatctg tgcaccggcc
     3661 gcatcatccg ccgaggcggc ggtattgtcg ccgagctggc gcgcctgttt gcgtagtgcg
     3721 gtcatttcgg cggagtcttt tgccactccg agcacggcca gcaattctga gtttttctgc
     3781 gcaaactcat aacctggcat cagcaactta actccggcca tcgttcccgc cgccgcaatc
     3841 cccacaccgg cagcgcccac tgaggccata tttccggcca gttccttgcc tgcctgataa
     3901 cgctgtttta ctgcgttaag ttttgcctgt tgcgcactga cacgcgccag cgcgtcacgc
     3961 tgacggttaa gctgtgcggt ggtttcactg atacggtttt tcagtccctg ctcatcatgt
     4021 gcaagattgc gggtattaat tcccacaacg gccagttccc gctgctggcg tttaacggaa
     4081 tccgtcaggc ggttatattt cgcctgtaag tcctccgccg cacgcttcgc ggattccagc
     4141 actttcgcct gagcacgtgt cggacgttcg gtgtttttaa actgtgtggc aagggcttcg
     4201 gcctcctgcc gtgccttttc aagtgcatga ccagtcacgg cgagctgtgc actggtcttg
     4261 cgaaatccct caatacggga tgcctgaccg ttcagctcac gcagtgattt ttgtgtttcc
     4321 cggatatccc ccgacagcga tttgctcgct gtgcggatgg atttaaacgg gcgggatgcc
     4381 tggtcaacag ccctgagcaa tacctgtaat tttacattgt tactcattcg tgtttccgct
     4441 tcgccggagc gccttttcgc gccatgtgat gagttcggtc aggctcatgg gatacagttc
     4501 tgatggcggc cagtgaaata tcactgccac atccgccatc aggtcatcga ccgagagatt
     4561 tttcggaaac gtcactgcac cgagttcggc gacaaaaaac cgaccacctt accggccagc
     4621 gccacaaggt caggcagttc cagcgcggcg acctcctgtt cggtcagcat cggtgccgtc
     4681 atgcgcggca gcactttaat cagtgcatcg acttcggagt tcgcgaccgc agccagactg
     4741 acaccgcgca gcgtcccggc attgggtttc atcagcgtga cctgttcgat aacctgctca
     4801 ccacgcttga ccggattgtc cagggtaatc acattttctt tgttcatggt tttctcactt
     4861 ctgaatcagg gttaaccggt cagcctggct gaccggatga aaatcacagg ccgatattgc
     4921 ggcggtgttg ctccagccgg tcgacgccgt tcaccttctc aatcatgttg atggtgtcga
     4981 tttcgaccag ctccttaccg tccatcgtca gccggaaata ggtgcagacc acggagattt
     5041 tcgactcagt gtcttctccc tgtttaccct cgccagtgtc gatttctttc tgacgtccac
     5101 gcatgaccac ttcgacggcc accgtttcgc cggtatcgtc acgctggtaa gagcctgcaa
     5161 aacgaatcgg cacagcatcc acaccggttg cggcgtaaag ctcccagata accgaatccg
     5221 ggaagccacc gagtgaccac tccattgaca gcgcatcgtc atcaaggccg aggtctaccg
     5281 gtgcgctgcc gttcatcccc gcaccgcgat agttttcgag cttacgggtc agttttggca
     5341 gcgtgacgga ctttgcaacg ccctgatagc tgtagccgtt cagaaagacg ttcattaact
     5401 tgagtttgcg cggcattgcc atcggtcagg ctccttaatt gctgttaacc gaggtgacca
     5461 gatttgccag gtatttatcg gtaatacgct ggcgcagggt caggttttca agaggaggca
     5521 ccggggtata gtcgtagtcg atatacagtt ttccggcctt aagggtttcc gcatcgttgg
     5581 attcttcgct gaaccagcag gtcgcatcca cgatatagcc gtttgttttc agctcacgga
     5641 atttggcatt gatgccgtca acgatgtcgc gaatcagcgt tgcggtgatg ggcttgtcca
     5701 ccgcccacat gtgcgcctca gccatcgtgt cggccagcac ctgcgcggtg cgggtgtagt
     5761 tttcaaagag gaacagcggg tcatcagagc aggtacggtt accccagaat cggaaaccgt
     5821 cgcggcgaat cagcgtagtg acgcctgact cgttaagcag gtcagcatcg gtgccggact
     5881 cctgcaaatc ccagaataca gatgcgctga tgccggtaac accgttcacc ccgacattgg
     5941 acagcgtttt atgccagccc tgctcctggt cgattctggc acgcagaccc agcgcacggg
     6001 cggtggcata cgcggtggcg gtggtgctgg tgaccgtatc ccatgcgagg aaatccggcc
     6061 agatgaccat cagctcacgc tggctgaaat tctggcggta ggctttcacc tcggaaatgg
     6121 ttttacagcc ccatgcgctg atatacccga aagcgcgcag cttctgacag actgatgcca
     6181 gtgcaacagc cacctctttg gtatccagcc ccggcacgcc gagaatacgc ggtttaacac
     6241 cggttaccga ctccgccgcc agcagggctt tcagtccggt gtactgaccg ttttcgtcgg
     6301 tggtgccgat gatattggaa acggtctgcg caagtttcgt ttcctcgtcg tcgccggtgc
     6361 cgtcttccac acgcacgaca acggtgaccg gttttgactg gtcggcgatg gcctgcaacg
     6421 atgccgccag cgtgcctttt ttaccggcct ttgcaattgc gctctgcaca ttggtaatca
     6481 gcactggttt attgaggggg aagatttccg catccgcatc gctggccgtg cagaccatgc
     6541 caacaatggc ggtggatacg gtggaaatga cgcgggtgcc gtcgttaatc tccagcacct
     6601 gcacgccgtg atgatagtca ctcatccgtt taactccgtg gttaatgggt gcaactattt
     6661 tctgttgggc agtgcatgag acgctatttg acctggctgg tcagtggatg aaacaacaga
     6721 taaagaaaag gcaggcaatt cgcccgcctg tcctgatttg tactcactca ttttccgact
     6781 gacaatttac atagccaaaa cgctatcaaa tctgacagtc tgctttgagc gaggagcaga
     6841 ggttagtttt agttaaccaa aatgataaaa agcagtagaa aaatccgctc attacgttat
     6901 ggttataagc cgcacataat catccgagcc aaatcctctt gatttcaaag aattagcact
     6961 tgctcttcac tagaaactat ggttctgact tcacgctcaa atattggatc cattatcatt
     7021 tttctatttg ttggaatagt aaatttaccg agtaccatga caatgctact caacggaaat
     7081 aattttggag taacatttcc taattgaatc gtaggatata tattaaagcc cccttttatc
     7141 ctcattggcg aaccttcaat gaagtgtcga taattaaatt tcgccatagg gattctcgcc
     7201 acatgcactt tcataattac agcaacctga gctattttta ctttctggag gtaagttgac
     7261 aatcgttcta actgcgttgt ttcatagaaa aaatcagacc acgttacagt acttgttcca
     7321 gtaacaattt ttatgctgtt tctcttgttg gggtgagctt ttccatattc atagatctcc
     7381 cgtaagctat ccatgttctt cacataaggg gctttccgac ctctcttgac ataaacgcgg
     7441 tctgtagtat cagaaggtgg atttgcctgc atagctgcag cttttctgct taatttagag
     7501 atatcatctt catcgagtat atgtatacgg aatatattat tacctgtggc aagcgcacgg
     7561 ctgatatcat gactggaatc acttgcataa attgtattca atcctgaagt tccatactgg
     7621 cataatttgt catgcgtgaa atttttttcc agtctaaaga aggcggggat atgcaaggtt
     7681 ttattaaaac tccgacgaat atggcttttg acatacacca aatgagcaga gcaatttggg
     7741 tcaggacatt tgagtggata gggattaaca ctataattaa aagtatttac atttactaaa
     7801 atctgattgt tatcaatagc ctgagttata cgaataccac tcattactcc tccataaaga
     7861 taggcgcaag catttagtaa taaaccatta acatgccgag atcatacccg atcattgaaa
     7921 tgtaaacact cccctaagac ggctgctggc ataatatttt atgacatcag caatgcccac
     7981 ctctggcaca gagtggactg tcagattagg ctttactctg tgccatagat atgtaagccc
     8041 acactagagc tcatacaact tattgcggca tttccggcca ttcaggattt gcaggatcca
     8101 cacgactgac cagaacacta tagcgttccc atgcctccag tcgactacgc tcctcatctg
     8161 ttgccatatt cagcctgacc gcgcgctcca gcggcaaaat cacggattca gcttcggaaa
     8221 gcaaagctgc cttatgtaat tccgccagtt tctgctgttc gtctgccgta taaatccgct
     8281 taatcacggc accatcctta aacatccatt tacctgagtc gtcagcacgt cggttggagg
     8341 taatatcagg aacctcaaca acgctaaaac cttcaggatt aagcgttgaa gcatctctgg
     8401 tgatagcgac aataatatta ttttcatcgt aaacaatctt tattgtgtct ggctgaaagt
     8461 tcttcacttc ctcataccag ttttttccgt cctcagagta aagccagata actccgtgtt
     8521 tctttgttaa ctcatactgt tccagtgttt tagcgttacc cgcttttatg ttctttaagt
     8581 gcatcatatt aaacgctcgc tacattatac caggtgccat ttatatactt ttgaacgggt
     8641 ctgtaataaa cgcccgctat attatcggca gagttggacc ctgtatcctg aacattaata
     8701 ccagacaata catgacctga cgggcactgg aaattccatg tttgccagtt gttcactcca
     8761 taatattgct gtgacccaag tcgaacatct ttcacatatc tggaatcaaa attgccatag
     8821 ttgccaggaa taacttgcga gccacaaagc cagttaccgt tattatccat gtacgcctga
     8881 ccatcggtgc cattggctgt ccttgagtta ttaatcatgt agatgccaaa ttgcttgttc
     8941 cccagtccac ctatcatgaa cttgcggtca gcgtgattct ggcgaagcaa cgcctgagcg
     9001 ctatcagtgt taactatatt tttcccaaag atagcgttgt tatcgcgcat ctgaatccac
     9061 ataccattac tgctgttaat agcaaaacgg tctgaaagcg tctcccctga aacattaagg
     9121 ccacgcccca tcgaaacagc gccagtagcg ttattaatcc ataatggcct taatccgtta
     9181 taagtcccca tgttgtcacc ggagtttgtc aacatgaagt atgtatttga accatcatta
     9241 cgaataaaga atccgtaatt accataagca atacgtaggc cgtttgctga ttttgatatg
     9301 acttcgccat ttacaatggc attgacaaga acatacaaag catcccattt aagattcatc
     9361 aggtcttttg ttgtagtact ctggcggctt ctccatttga aatattcatt gccgttatcc
     9421 cccgtttcaa accacatgaa tgaatcagtg tcaccatcgg catcattttt aaatccaatc
     9481 ttcgcccagt cagtatttcg aatccaggca aggattgagt cgttttcaaa agtaagtcca
     9541 ccggacaagg tatcgccatt tttttgcacg gcgttcccgg ctcggtttac cgtttcctgt
     9601 aaaccgagat attcgataac ggcggcaacg gtcgatttag ccagaatatc ccgcccgact
     9661 tttgtcaggg ttgccaagct ggcaacatca ttcccaataa aatacggaaa cctgtctgcc
     9721 gcagtagcaa gccccgccag tgccgtcagg gtggcatctt tcggttgctt acccgcaagc
     9781 gcattagtca tggtggtagc aaaattcggg tcattgccca gcgccgccgc cagctcgttc
     9841 agcgtattca gtgcgtcagg cgacgagtct acaagggcgg caatcgcggc cataacgaaa
     9901 gccgtgtttg cgatctgagt attattcgtt ccctgtcgcg cagttggcgt cgttggcgtt
     9961 ccggtcagtg cagggctgtt taatggcgct ttcttgttcg tttcatccat taccgtctta
    10021 acggctttcg gcgttgcagc cagtgtttca gacgtgctgt tggtcgcact gcttaactga
    10081 gtaaaacctt ttgcggtcag cgaggcgtca gggtgacgtc gtgactgttc atgttctgca
    10141 attttgtcat caacgtaatc ctgcgtcgcc atcaccgttg tggtgtcgat ggtcagatcc
    10201 actgaggcta cactgctgac gataatgacc atgcggcagg tctgcgaacg tcctgagcct
    10261 tcggcaaggg ctggcttata actttcggcc atgttcgcca cggcaattaa cgttcccgca
    10321 tcatcgtaca ggccaagctc acgcatccag aaaccgccca cctccggcgg aataaccagc
    10381 tctgcgataa tataattact gtttcgtttg tcctggctga ttttgttcag cgcatgtcgc
    10441 cagacttcgt ggataagccc ggtctgtccg gcatccggga caggcaattt accaccgcca
    10501 tccccgacgg ccatcgtggt aatgttgacc ttccgccctc ccggcgcggt tgccgctgcc
    10561 agctttgctg caccggcagt ggtgataacg gttttgaatt ttgtgctcat tattcctcac
    10621 ttatccgggg taaaccgtaa ttacatcgcc gtcataagcc acaccaccgg caaacaggta
    10681 gccgggaatg tcccgggtaa tgttcagacc aataaggtgg cggcttgcag gtttggcatc
    10741 agcaatcagc cgttccattt cctgatacat tgcctctgtg atgccgcttt ccagtacacc
    10801 aatatcaagc cggaaggtgc cgggcgggtc actgttttcc caccactccg tcacgttgat
    10861 gagatagccg agcggctcca ccacacgccg gattgcacct attgtgcctt tatgacagtg
    10921 gatgaaatag gcatcgcgga taacggcgcg ttttgtcgct tccggccact tttcatccca
    10981 cctgtcgacc gaaaacgccc acgccagcca cggcagcaga tttgccggac aggtatccgg
    11041 gttccacagc tcacgaatac tgaccggcgt tttttcaatt tccgcacagg cttttgcggc
    11101 ggcgacttca agcggtgatg agccggtcgg cagcagtcgc gaatcactca tccgagcctc
    11161 cggtcacgac gctgtattcg gtacagaaag acgcctgcgt attgttgagc acgatgtcgg
    11221 ccagcggtgc agccagttcg acacgctgca cgccttccac atgcaaagcg gcataaatgg
    11281 cagacagacg gatgtcgcgc cccagccggt gctgtgccgt gatgtatgct tccagttttt
    11341 tcacggcggc cgcgcggatg ggttcacttt cgggaccagg gtaaaggtaa agcgtggcgt
    11401 ttatctggta ttcaacaatg gcggcagact gcacggtcac gcggtcggcc accggtctga
    11461 cgtcctcgcc attcagggcg ttacgcacca ccgccagcag gtctttggat gcgacaccgt
    11521 tattctcacg tgacagcaca gagatggtga cgcaggccgg agacggactg gtgacagaga
    11581 tatccgcgac acgcccgtca gcactgcgac catgatactg ataggcaccc accgacccgg
    11641 cgacgcttaa accttcaaac gcctgctgaa tacgcagacg ataatcggta tcagattcca
    11701 tcactgccgg tgtcggcggg atggtcgaat catctgccgg ggtgatagtc agacgcgtgg
    11761 tgttgtaatt ggcaccaatc acatcaaggt cattaccggc ggcacaggcc agcatcaccg
    11821 cccgtgcggc ctcattcaca cgctgacgcc agataagctc acgataagca ttttcttcca
    11881 gcagtttgac gagaggctca gattccagtg tcagggtacg ggcgaccgcc tcctgctggt
    11941 cttccgggta aagggaaatc agtgtcgcct tgcgttcggc gagaatggtt tcaaagtcca
    12001 gctcctcgac cacatccggt gcgggtagct ggttcaggtc gataatcggc atggtttcaa
    12061 ctcacaggga tggttaacga aagtggctgg ccggtgtcgt tgtgctgacc ggttaacgtg
    12121 accgtcattc gcccgtcaaa actgcgcgcc gtggtgacgg atgacagggt gacgcggggt
    12181 tcccatttca gcactgccat gtaacaggcg accttaatct gcaactcaag cgccggggtc
    12241 tgcggctggt caatcattga cgccagcaac gagccgtaat cacgacgcat cacccgtgag
    12301 ccgacggggg tacgcaggat atcgccgata ctctggctga tatgctcaag gtcagtgaca
    12361 gtcaggccat cactgcgatt cattccgaga taacgcgcag tcatagaggt cccccggttg
    12421 tgccgccgct gtcgccgggg tgtttatggg tatgcagtac cttaccgttt gatgagagct
    12481 caccgccggt gtgttcaatg ttgccgcgca tcgtcccgcc cttctgcact tccagcgtgc
    12541 cggtaatcag cctgttggtg cagaccacct ccggtgtgtc cagggtgatg cgggttgatg
    12601 ctttcaccat gaccaccggc accgtggcag taacagaatc agaagccgtc acgctggccg
    12661 ttttaactcc gcttaccgtg agcgcgctgg tttcaggttc atactcaatc accgccccgt
    12721 cagggaaacg gatatgcagg gcatccgccg acgcagacgg cgcggggtta tcgccggaat
    12781 aaatccccgg cagaacgaac gccgtgtcga gttcaccacc cacggccaga atcagcacct
    12841 gttcccccac ggaaggtgcc caccatgtgc gcgaacgccc ggcgcgatgg gtcagccact
    12901 gaagccagtc ggtgcacatg ccgccggtct gcacacggca gcgaccggca ttaaggtcgg
    12961 tttcgacgat aatgccggtg cggatcatgt tgcgcagtgc gcgcgcgagt tcctgaatat
    13021 ttgcgagagt gttcatgcgt gtgagattgc acaatatata aaagttatgc tatctggatt
    13081 catttgtaga acgaccatac aacattcgag gagagcgtaa tgttcagtga taatgtgact
    13141 aatgcgtggt ggtttatctc tttgtatcta tttttattaa tagcattaac atttgttacc
    13201 tttggtaaaa gtaatcttat gaggtttatt gcacatcatt tcaatcttga gtattcagac
    13261 agaaagttaa aaatgctcga caaaaaatgg cgcgacattc aactatttaa aataattaac
    13321 ggaatcaatg tatcaggcat cgaagatgtg agaatgatac agcaggggct gattgatgga
    13381 aaactaaaaa catcgtattt tttccttact cgcttctggg gtgacataac aaaaccacca
    13441 cacataatta aaacaacaat tgtaattctg gccagtatta tttatattct cttcgcatgt
    13501 tatatacaca atgaacaatc cgctatagta agggatgcca taggtatacc atataaaaat
    13561 atgatgtact atgtttatag tgacaaagtt cttttatcct tcaaaaataa aacggttgaa
    13621 tttaataaaa cttatagcct tgccgattgc aagagtctgc aaaacgtatt tataaaagac
    13681 acacttcccg agatcgcctg caataagctc ttacagctaa acaaggagga ctccgaatgg
    13741 ttaagtcagg agattaaaga taataacagc cacagaaaaa cattattaat aatatcccta
    13801 acctatttca tttcaggtct gcttatattc ctgtcatata caaaattcct ttacgccaat
    13861 aagaaggttt tagaatacaa agcatcaaat aaaaaccact cataaacctc taaatattga
    13921 gcgaccagca cggccgctca atgcttaatt gcgcatcagc ctctgcctgg ataaaactaa
    13981 cgctcaaggt gagccaggat aatctcttca atcatctgca catcctcacc ggtaaagccg
    14041 agcagaggac gcgccggata atcaattttc ttaccgtctt tccggttttc ttccgacaga
    14101 ccgaactgat gcacactggc gattttcggt gacttcccgc cgtaaaactc cattgatgcc
    14161 tgttccgggc tggcgcggat atgcaaaaaa cgactggtga taagtttcgc aaacattttt
    14221 cgcttaacac gaccggtctt ttttctggcg ctctgctgct ggcgtggcgc gtagggtgtg
    14281 ccgtccggag ctttctgtgc catcacccga cgctgctgac tctgccgcag gcgtttcgcc
    14341 agttcggcac tcagtcgccg acgccctgac ggtgacagcg attcaatcag tccggtcagc
    14401 cggtcttcaa aacgcttaaa ctcattcatc ccacttgctc accagttcgc cattgatata
    14461 cagctccatc gggcgggtga ccggctccgg cggcggaggt tccgggatat tcttcacatg
    14521 cagcgcgccg tccacctcac tgaccagcgt gcgctcggtc agcatcaggc tgatactgat
    14581 atcaaagctg ctgtcattgt tgatgtctgc ataaaacgtg aagccctttt tctggcctgc
    14641 gtcggtggtc atgatgtcgg gctgattttc ccgcagccat gccagcaccg gcacgatgag
    14701 caggtcaaaa tcaccggtaa agtcggtcac aatgacattg agcgtgtaac gcttttcgaa
    14761 tgacagcgac gtcgccagtg tggaggcaat actcccgtta tccacgaata tccgcagcat
    14821 ctcggggctt gttttcagca ccgtgacggc atcagtcagc gccctgcgca ggctgtcggg
    14881 tttgagcatc gttttcgtcc tgacagtgtt taatcatttt tacctggctg gcacagcgtg
    14941 ccagcgcgtt ctcaagctgc cggatatcgg cacttaaatc accgttcgtc tgcgggtcac
    15001 tgcccggcat caggcaaagg ctcactttcg ggcaggcgtt gtggacaatc actggcgtcg
    15061 gtgcaggccg ggcgctggtg caaccggcgc acagcatcag gcaggccagc gccgtaccag
    15121 cggcgaaaat cttcgttttc attcagtaac ctcgtgatgg ttttctcgcg ctgtgcttca
    15181 cgcttcgccg cgttctccag ttcctgacgc agtgccacct gcgccagctc gtttttgtct
    15241 gctctggtga gggcaacatg aagctgattt ttcagcatgg tgatggtcgt ctgctgcccg
    15301 ttggcgacgt tgttcgccct gtccagcgag gtgcgcaggc tggcgttttc atgcttcgcc
    15361 agaaacagac cggccaccgc cagtgataac aacacaacca gcacaatcat cagctttgac
    15421 atggttcccg cccctcaaaa cgctgacgac aggccgtgcg tatcagccgg aagaacaccg
    15481 acgccacgag gtaaatcagc gcggtaaaaa tccacccggc agcgaccagc gagataaacg
    15541 ttgccaccat cactaccaga gccactgacc gcctgcgcca cggcaccggc tgcaaaaaca
    15601 gcgccgtgac aatcttcacg gccagcgatt ccggcggcag ctcccgcccg tagcgttcca
    15661 gtacatactc agtggcatac acgccgacac caccggcaac cacacagata accgtcgcca
    15721 gaatcgccca ggcggcgaca aaactgacgg ccacgctctg cgggtaaatc agggacagtg
    15781 ccagcatcag cgccagcgac acgttcagca tcagtgaaag ggataatttc ttcatggtgt
    15841 ttactccgtt taagccagta cgccgccagc ggtacgccag acggtgaaca gtttttccag
    15901 tgaatgctca cgctgaccgt aaccggcacc cggcagggac gcccagatat tgcgacagcg
    15961 tgaaatggcg cgctcaatgc gtcccgcccg gatgtcatcc agtgcaccgc gttcgcggat
    16021 caactgaatg gcgagtctgt cctgtgacaa cggactgaaa tcaggcaggg caagctgttt
    16081 gcggtagtgc ggccagaaca ggtaaagctg ctgatagcga ccggaggccg tggatttttc
    16141 accgcgacgg ttaaacacct tcgccggtcg gccatgcgcg aacgggtggt cactgtagtc
    16201 ggtgaaaatt tccggcttcc cgtccagtcc ggtgactatc acgtcatagc cccggttttt
    16261 cgtcagcgga tgattcgccg tcccttcgga cacggccagc atgtcgagaa aggcggcgat
    16321 attctggtgc gtgttaatta ccggcattac ggtttccccc tgcccttaaa gcggcgctga
    16381 atggcaatct caatcacctg ataaccggcg atacccagca tggagccgat gccgcaaacc
    16441 gcaggcagtg acaggtcagg aaactgcacc agaacaacac cggcaaccat cgagacaaaa
    16501 ccaccgagca acatgcgccc gataaacaga cgcggggtga tgggttcacc accggtaagc
    16561 accttgccga caacaatcag caccccaatc atgaaaagcg acaggacgct tttttcttct
    16621 gctgtcatgc gttactccca cagattgaca gtttcagcca cgggcgcggt ctgaacgtcg
    16681 ggcagttcga cggcggtgcc atgtggcagc accgcaccca gttcagccag tcccggattt
    16741 gcgacgagca cggtctcaac cacgccctca gtgcgcccgt aataccggac acaaatggcg
    16801 tcgagcgtgt cgccctgtag cgcaaaggtc ttcatcagat ttgactcacg atgcagcgcg
    16861 gcttgtcctg gatacgcgcc accgcccagc gcatatcccg ccacagttca tcaatggtgc
    16921 tgtcaatgct gtcagccttc ttgtcacctt tcgcactggc atccacgccg cgataacgct
    16981 cataaagcga cgcggtcgcc atcgcacaca cggcgcgctc gtagtaaaaa actttgatgc
    17041 tttcaccgtc gatgtcgtcc gccgggacgt ccgccagacg cgtaaaaccg gcggcaattt
    17101 tctgttcgcg gtactcgtac agctccgcat tcgtctccgc catgcctgac ttgatggcct
    17161 cacgcagacg ggcgggggcg acggtctgct caaggcgcat acgttcccgg acgcgcttcg
    17221 ggtcgatatc gggaaaaaag aacgtgtttt taatcaccgg ctcgtcgcct gccggttgcg
    17281 ggatgaccac cgtaccctca ccggacacgg gagcctcctt tcgcggaata atcagcgtca
    17341 tcatgactac ctctgaaaag tcgggcggtg gacgccggtg caatgtcagg taattcaccc
    17401 tcactgaccg gcgtgccgcc ctggcgcggg gcgcattcgg ttgttaactg gctttctttt
    17461 tcgggcgtcc acgttttgcc ggtgtcacgc tccgggtctt acgcggggta cgggtggccg
    17521 cttttggctg cggctccggc ttcggtttca gctcccgctc cagtcgttca atctcttttt
    17581 tgacgcctgc ctgacagtcg agctgtgtcg cacgttgcag gtgagccagc gcaccggcgg
    17641 catcaccagc gtcacgcaga aacagaccgg tgattttgtg cagctttgcg cgcacttcat
    17701 caggcatgtc agccgtggcg gtcagttcaa tggtctccgt cagcaggcgg gtatccacag
    17761 actcaccggc agcgtgagca cgcatggccg cgagcgccac ctcctcggtg aacatgtacg
    17821 gcggggggcg gcggtgttta cccggcatgg tcagaccgta cttcagggca taacgggcaa
    17881 tctccagcgc accggcaata tcgccggtat ccagacgcca cagcatgacc gtcatcagaa
    17941 tgtcatcctg tgcacctttg ccctgctcca gcacgccgtt cacccacggc aaccagaacg
    18001 gcagcagttc gcgttttttc gcggccttca gctcttttga ataaatcgct ttcagtgtgc
    18061 gctggtctgc ggccagcttg accagcatct gctcatagac agttgcatgt cgcagcgggg
    18121 cggcttcccg ctgcgcggtc atcgctgccg agacccgcat catgtggcgc tgtgcgggac
    18181 tcgtcatcgg tcacgctccc ggctctgcgg tcgccctggc cggtgtggag aaatcaccga
    18241 ccttaatttt ttccaccaga caaccggcgg cgtagtcttc caccacgtaa tcaatgttca
    18301 ttgactcgta gttctccacg cggtcgagtt tcgggttttc cacaatcacg cggcgatggc
    18361 tgtcatccat gtagtagatg gacaggtttt ccagcttcgt gatgagcatc gcatccgccg
    18421 ggaagtacgg gacgcgtacc gctggcaggt tgccgatgcg tttctggctg atgatgacgt
    18481 cagcggccag catttcgctg ttgtcctgct ccttgttgac gatgggaaaa tacttgtccg
    18541 ccagtagctg acgtcccaca atcaccacaa ggtcagggtc ttcctgatac cacggctcaa
    18601 tcaggttgtt ggtcgcatcc atcaccagtg catcaaggct ggcataatca ccgcccttac
    18661 ccacgcggat gacctcagag gtggtgtgcc cttcctcgtc agtgaccttg ctcatcacgc
    18721 gcgccggggc ttcattgcgg tatttctgca gccagccgac cgccacatcc tgcagcatcg
    18781 gattgctgct gcggtcagag gtttcggcac gcttcacgcc gttaaaaccg gccatgatga
    18841 aatcaaggga ctggcgtttg ataatggcgt tacggacacg gagctggaaa tcctgataac
    18901 gcgcccacag gtccagcgtt ttgtagcgga tataaaaatc gaagttaatc tggtcgcatt
    18961 cgtacttgtt tgacgccagc ttcgagaagt ccttcggctg acgctcggtg ccaccggcgg
    19021 tgtcggtggt gctggcgatg gagccggtga caccaatacc aattttttcc cctttcattt
    19081 cgctgaccgg cacaatgttg atgcgggtca gaaagtcaga ggactcctgc atggtgttca
    19141 tcagggtctg ggtgaccgac ggttcaacgg tgaatttttt cgacacatca ccggcgtcga
    19201 tgccgttcag ttcggcaaca cgggacaggt aggcattaaa tttaaagcgg gtttcctggc
    19261 gcatagtttt tcctgaaatt aagggttaat cgtgaaggtt ttcccggact gactgacgcc
    19321 ggtcagcagt tcgtcatcag ggcgtcaccg ccaccgccgg tggccttgct gcggcgctgc
    19381 tgggtcagac tttcggtgtg gtcgagactg tttttcaggc gggtgaatgc ctggctggtt
    19441 tcatccgccc tgtcagtcac ctcttgctta agtgcggaaa aggcggtttc catctcagcg
    19501 aggcgctgct cagtggcgct cagtttttcc tgcacatgtt cagcaacagc ggtcaccgct
    19561 tcatgcacgt cattcagacg ggcgtcatcg ctggcctgtt tgcggccaaa aatggatttc
    19621 accttttcgg tcagggcggt gaacacggtt tcaggcaggt cttcaaattc cagctcaaca
    19681 ggcgttgcca ctgaaatcag gttttcaggg cttaatttga agcggttcag agggttgtgt
    19741 tttgccgtgc ggcagaattc caggtattcc gtgccgaggc ttgccgggtc atcggtgacg
    19801 gccagaccca ccagataaca tttgccggtg ttggcaaagt tcggctgaat ttccattgag
    19861 gtgtagacct tctgtgcggc cttgttcatc gcgataaggt catcggtcgg ggtgattttc
    19921 gcaaacagcg cccatttgcc tttcagcgcc gaatcatcgt caatcttttc ggccttcagt
    19981 tcgaccacat cgccataacg tttaaaaatg ccgtcaggca ggatgcctcg cagatgttcc
    20041 aggttaatac ggcaaccata gacacgcggg tcaaaggttt ccgccatttc ctgaatatcc
    20101 tgcgcactga tgacacgccc gtcacaggtg tcaccctcaa cgccgatacg aaagaatttt
    20161 gagacttttt ttgccattgt caggagtcct gaatagtgat tagaggagtc acatgtcggc
    20221 atcagtttcc cgacgatgcg catcctccgc catcagtccc ggatggctta tcactgacac
    20281 aacagcacct tagcgaatcg cggggcgcga ctcagtagcc ttgccgtgta ttcatcacgg
    20341 cgaggtattc atgaccatca ccacagacac cactctttta cacgacccgc gtcgtcaggc
    20401 ggcgctgctg tactggcagg ggttttccgt gccgcagatt gccgccatgt tgcagatgaa
    20461 acgcccgacg gtgcagagct ggaaacagcg cgacggctgg gacagcgttg cccccatcag
    20521 ccgtgtcgaa atgagtctgg aagcgcggct gacccagctc atcatcaaac cgcagaaaac
    20581 cggcggtgac ttcaaggaaa ttgacctgct gggacgccag attgaacgac tggcacgggt
    20641 caaccgctac agtcagaccg gcaacgaggc agaccttaat ccgaacatcg ctaaccgcaa
    20701 caaaggcggg cggcgcaaac cgaaaaagaa ttttttcagc gacgaagcca tcgaaaagct
    20761 ggagcagatt ttctttgagc agtctttcga ctatcagttg cactggtatc gcgccgggct
    20821 tgagcaccgc atccgcgata tcctgaaatc ccgccagatt ggcgcaacgt tttatttttc
    20881 ccgcgaggcg ctgctgcgtg ccctgaaaac cggtcataac cagatttttc tgtcggccag
    20941 taaaacgcag gcgtatgtgt tccgcgaata catcatcgcc tttgcccggc tggttgacgt
    21001 tgacctgacc ggtgacccga ttgtcctggg caataacggc gcaaaactga tttttctcgg
    21061 caccaactcc aacaccgcgc agagccataa cggcgacctg tacgtcgacg agattttctg
    21121 gatcccgaat tttcaggtac tgcgtaaggt ggcatcaggt atggcctcac agagtcacct
    21181 gcgctcgacc tatttctcca ccccgtccac gctggcgcac gacgcctacc cgttctggtc
    21241 gggtgaactg tttaaccggg gacgcgccag cgccgccgaa cgcgtggaaa tcgacgtcag
    21301 tcataacgcc cttgccggtg ggcttctctg tgcggacggc cagtggcggc agattgtcac
    21361 cattgaggac gccctgaaag gcggctgcac gctgttcgac attgagcagc tcaaacgcga
    21421 aaacagcgcc gacgatttta aaaacctgtt catgtgtgaa tttgttgacg acaaggcgtc
    21481 ggtattcccg ttcgaggagc tgcaacgctg catggtcgac acgctggaag aatgggaaga
    21541 ctatgcgccg tttgccgcca atccgttcgg ctcccgcccg gtatggattg gttacgaccc
    21601 gtcacaccgt ggcgacagcg ccggatgcgt ggtgctggca ccgccggtgg tggccggagg
    21661 taaattcaga atacttgagc gtcaccagtg gaaaggcatg gactttgcca ctcaggcgga
    21721 atccatccgc aaactcaccg aaaaatacaa cgtcgaatac atcggaattg atgccaccgg
    21781 cctcggtgtc ggcgtgttcc agctcgtgcg ctcgttctat cccgccgcgc gcgatatccg
    21841 ctacacgccg gaaatgaaaa ccgcaatggt gctcaaggca aaagacgtca tccgccgtgg
    21901 ctgtctggaa tatgacgtca gcgccaccga catcaccagc tcgttcatgg ctatccgcaa
    21961 gaccatgacc agcagcggac gcagcgccac ctatgaggcc agccgtagcg aggaagccag
    22021 ccacgccgac ctcgcctggg cgaccatgca cgccctgtta aatgagccac tcaccgccgg
    22081 tatcagcacc ccgctgacat ccaccattct ggagttttac tgatgagcaa gaaaaaaggg
    22141 aaaacaccgc aacctgcggc aaaaaaaatg accgccagcg ccccgaaaat ggaggcattc
    22201 acctttggtg agccggtgcc ggtactcgac cgccgtgaca ttctggatta cgtcgagtgc
    22261 atcagtaacg gcagatggta tgagccacca gtcagcttta ccggtctggc aaaaagcctg
    22321 cgtgctgccg tgcatcacag ctcaccgatt tacgtcaaac gtaacattct ggcctcaacg
    22381 tttatcccac acccgtggct ttctcagcag gatttcagcc gctttgtgct ggattttctg
    22441 gtattcggta atgcgtttct ggaaaagcgt tacagcacca ccggtaaggt catcagactg
    22501 gaaacctcac cggcaaaata tacccgccgt ggcgtggagg aggatgttta ctggtgggtg
    22561 ccgtccttca acgagccgac acctttcgcg cccggctccg tgtttcacct gctggagccg
    22621 gatattaatc aggagctgta cggtctgccg gaatatctca gcgcccttaa ctctgcctgg
    22681 ctgaatgagt cggccacgct gttccgccgc aagtattacg aaaacggcgc tcatgccgga
    22741 tatatcatgt acgtcactga tgccgtgcag gatcgcaacg atatcgaaat gcttcgcgaa
    22801 aacatggtga agtcgaaagg ccgcaacaac tttaaaaacc tgtttctcta tgccccgcag
    22861 gggaaagctg acggcattaa aattatcccg ctcagtgaag tggcaacgaa ggacgatttt
    22921 tttaatatca aaaaagccag cgccgctgac ctgctggacg cgcaccgcat cccctttcag
    22981 ttgatgggcg gcaagccgga gaacgtcggg tcgctgggtg atattgagaa agtagcaaag
    23041 gtctttgtcc gcaatgagct tatcccgtta caggacagga tccgcgagat aaacggctgg
    23101 ctcggtcagg aggtcatccg atttaaaaac tactcactgg acactgacaa cggctgaaca
    23161 tcgccgcctg cgggcggctt ttttacaccc cgtcatcacg ccctcacacg ttcgccactg
    23221 tacaaaacac cccgcagaca caccaacgcc ccggcaggcc gactaaacgc catcacgacg
    23281 cgctcagacg ctgaaaaaat aaaatcagca ccaccgccag cgcgcagtgc tttccccgcc
    23341 tcgcccgccc gcttcatggg tcggttttga tgcaattcca aaagccgtcc aaactctctt
    23401 aggctaaatg tccaacgaga aaatagttct ttgaatgtga atgcatttta atgcagagtt
    23461 atgcccagca tttttgtaca cttcgatgta tcaaatgcgc tgcaaacgat caaatatgga
    23521 tgttttatca agcatccccc aaaagatatt tacatcatcc catgaggtta agatggataa
    23581 caaaatcgta gaaattgaga caaataagct tgattttgac cctaaaaacc cacgtttctt
    23641 tcgtctcaat gatgccagta acgctgcaac agtcattgag gaaatgttag atgacgaaag
    23701 tgtccacgat ctaatgctat caatcggtca gcaaggttac tttcctggag aacctttatt
    23761 ggcagtaaaa agcaatggaa actacatcgt ggttgaggga aacagacgct tagctgctgt
    23821 aaagttgctc aatggagatc tgcttcctcc aaaaagaaaa cttaaaggtg tgcaagaaat
    23881 cattgatgat actaccaata aacctaagaa gcttccctgc atcatttatg aaaaccgaga
    23941 ggatgtactg agatatatcg gttatcgtca tataactggg gtcaaagaat gggactcatt
    24001 atctaaagcc aaatacctta aagagttatg tgatactttt tattcacatg agcctaaaga
    24061 gatagtatta aaaaatctgg ctcgtgagat tgggagtaaa ccacattatg ttgcaacact
    24121 tctcactgca ctgaacttat atgaagtcgc gcatgaccat gagtttttta atttacccat
    24181 gaaggcttct gacgtggaat tttcatatat aaccacagct ttgggatatt caaaaatcac
    24241 aaactggtta ggtctacagg ataaaaagga ttttttagac ccaaatttaa atgaagaaaa
    24301 ccttaagcgt ttattctctt ggttttttgt gcctgaccaa caaggtagaa ccatcatcgg
    24361 tgagtctcga agaataaaag atattgcagc agtggttgag aaacccgaag caattgaaat
    24421 tctcatgaaa agttcaaact tggatgaagc atatctatat accagcggag aaagagaagc
    24481 attagataaa gcactaaacg cagctagtgt taaattaaga gtagtttggg atatgctact
    24541 taaagctaaa gaattaacat tagagcatga agaggctgca tctgaaattt ttgagatgtc
    24601 aaaaaatatt agaaatcaga tcagaagcaa aagggaggat gattgagatt atgattacaa
    24661 atcttgattc aatgccttct aatgagcctt atttatgggc tgattatatt gagatattgg
    24721 ccttaactaa tatcgacagg tcattcagtc gaggagacct atatagcaca ctgcaagctc
    24781 aacccgaagc agtactagct gaaacagatg aagcagaaga agagggcgtt tatgatgttg
    24841 atgatgaaaa tgatacgcct gtacgcaaga gaacaaaacg aagtgttagt cgagcatata
    24901 ctgacagaaa gtggagctat gcgataggct tcatacgaca acgcattgat ttatttgggg
    24961 atagttaccc ttttacttta tcagaagaca acgatactgt agagttacgt gatatatcag
    25021 aaaagccact ggaacattta gaaagactat atttagcttt actaatctgt gctaacataa
    25081 aatatgtcaa cataatgagc agaagagaga taacgcgcag ttttgaacta attagtttac
    25141 ctatttttga aagcctaatg cctagcggta gcataataaa agcatgctgg gcttctggtg
    25201 gtcaagcggc cccttacact ggaactctat ataataaatt taagagtatt gcttccgata
    25261 tccgttgcac agcgaacttc aaagaacgag atttcagtcg aggaaatagt ggtgacggag
    25321 gccttgacat aattgcctgg catccaatgg gagatcaacg agatgccatc cctatttctt
    25381 ttgttcagtg tggctgttct caagaagagt gggaagcgaa gcagcttgag gcctcacctg
    25441 cgatgctcta cagtaaattc cccgtagctc accgatgggc aacttattat ttcttacctc
    25501 aagatctacg atggatagat ggtgagtggg cgcataaaaa taagttaggc gatgctattt
    25561 ttgttgatcg cctaagatta atcaatttaa ccagagcatc tgataatatt gatcacagtc
    25621 aaaatattag ctatctagat atcatccttg atccttccag cgcgatcgct gcttaatccc
    25681 ataaatctgg aaggtttcta gcaaccgcct caaacaatgg aggcggtact gcattaccta
    25741 ccacagtata tttcatatta atagaagccc gttcagtttc tgggaaaatt aaatccccaa
    25801 aaccttgtaa aatagcagcc tctcgatagc taaacctacg agctggtgca tccgaagtaa
    25861 attgccactt atcaggtccc aatttttcta atgttggact tattggatgt agaggcatat
    25921 gtctaggatt tgcaacaatt gttttagata tctgatccca atcttgccta cggtttcgcg
    25981 atagataata ccaatgaaaa tcggcgtcat aaaactcgcc aacaggccaa acaggcatat
    26041 gcccaatagc atcacgaatt gtggaatatg gtgtcaaacc atcaccatgt gttggttttg
    26101 gaaatttgta tgtaataccg tagtcctttc gtattcctac gataaagatt cgcttcctat
    26161 cttgggatac cccataatgg gacgcattca gaatttgcga gcttactgta taacctgctt
    26221 cttcgaaaac tttgaattga tcctttaata aatgctcaaa gttacgcctt accataccag
    26281 agacattctc tacaatgaat gcttttggct taattttact caaagcacgg gcaaactcta
    26341 aatatagtgt attaatcttt ctatctgcct tccttgcccc accttgacta aatccttggc
    26401 aaggatagca tccgatgagc aactcagcag aagggaacga ctggagccct gagatatcgc
    26461 ccaaaatgta gtcagtttca ggatggtttt ctaagtaaac gtcccttgcg taaggcaaaa
    26521 tatcatttgc cataagcaca ttgaaacctg cgttcaaaac tcccgcatca gaaccaccac
    26581 atccagaaaa aagtgaaact acagttggca ttgacccctc ctaaaaaccg accgcgtatt
    26641 atagcgaaac accccgttgg gaaaagctag attttgccaa gtcttgatat tctcacgttt
    26701 tagtagttgt ggccatcttt aacgagaaaa agataaaatt gacttctcat taattttcaa
    26761 taggtttaat tgtaagctca aactaacgcc tcgcgacact cgttattcaa ccccgccagc
    26821 cctgaaaaca agtttcacga ctggcggcgt tctctatcgt ctgcgttgtg gtggcgcaac
    26881 tctggactga ccgatatggt taaaccgccc gtaattatcc cggactattt cggcacaccc
    26941 gaccagctca tcgggcgtca gattttcgtt gaccataatc cgctgtaaac gctgaacaat
    27001 agccatcagc ttgatatttt tagttttatg gtgcggtatc tcgcctggta ttctgtgcat
    27061 tatccaagcc acccgttttg ctgtgcacgc tccatctgtt catctgaata gttccatgct
    27121 ccatccgtgg caaccattgc cccgccagac atccccgtct ctggttcata cataacagca
    27181 aggccgagct gatgcataat ttcatgatta attctgaata ccaggccacg ctcactaagt
    27241 tctttccagt tcacaatctc atatgcgcct gtattaagca gctcaatact tagcaagaca
    27301 taatcttcca gccagtctga caggtcagta acatctgtta tccgggcttc aacatttcgc
    27361 cccgtataca caccctgcac ccattcatac aaaatcaacg tgtccccgcg ctcataatta
    27421 cggtcatttt tccggaactc tgcacgtttc tttccttcca gcacaaggtc gaaatatttt
    27481 gcgtgcagct ttacctcgtg aatttttgcc attatgtcca ctccattact gttgagaatc
    27541 ccggccactc atcagcgacc ggatacgtga attttttccc gtcataattt acggtcgcgc
    27601 cacgcgccag cgcctcaagc tcccatcgct gcggcctgat accgttctga gcaaggtcaa
    27661 cgcggatacg ggtaatttgc gttcgttccg accgggtcag tctggccgat ggcgctattt
    27721 catgtggttt taacgggctt ccgtttctct gctgacgatt tggtgttctc agttcgtgtt
    27781 ttaatgcacc tctgagcgcc ctcacgacct ctggctcatt ccattcgata acaccgtcat
    27841 caaccagatt aagcacggct gcggcatgtt cataaggtgt gggagccggt aacgaagtat
    27901 cactgccggt gagctttcca cagttattga caggactccg aggcgcggcg atgccgcttt
    27961 ttaaagtcaa aggctcaacg accggaactt tcggcacaat gcgccagtcc gtcgttctgg
    28021 tgatatgaat atgacgcgcg ccgagatgcg gcgcgtaaat accgaccact ctctcgactt
    28081 cttcctcgta ctcgttaacg tcactcgacg ggctacgggc aaccctgaca gtctgacaat
    28141 cgcgcgggac atttgcccca ccctgcgcgc tgatatacaa cgcaaaatca ccactgtctg
    28201 cggcagcgcg agcagcctcg acgcgttcgt caaactcatc agcaatactg acgccgcgag
    28261 gtaatttgcg tagttcacgg taagccccca ttgtcggtag gccaaccgtt ttaaattgcg
    28321 gaatgcgcca cgttgacgcc catgcggtaa cagccgcggc agtgtctttc agcggcctgc
    28381 cggtatcgtt atcgagctga ccatccagtg catagccgtc gatgtttttt gaaatgtatt
    28441 tcgcgatata ccccgcagca ccgccccgat taaggtgttt tgcctgaaaa cggtttcgcg
    28501 cggctcctct ttcgtcgcca tcctctttga gcgcatagcg acgcatgatt tcgataatct
    28561 ggttacgctg gcgtggatta caaaaaagca tcatatgcca gtgcggtgtt ccgtcgtggt
    28621 gtggttcaac aacacgcaaa ccgtaggcct gtaaatcatt atccttgaat gccgtgcgca
    28681 tcaggctcca gatacggcag agataacgct gcgcatcctt tggattaaat gcctcatcgt
    28741 tccagccgtg attaagctga acggttttac tttcgccttt tccgacctga cgtgtcgggt
    28801 gatactttga cggcgcggtc agcgtgataa acatccccac atcaccctct gcggcggcgt
    28861 aacgctcaat accggcaatg gtgttcatca gctccatccg gcgaatttca ggattagaaa
    28921 tactgcccat caccttactg ataaggtcga tgcgctcgcc ggtttccctg ttttcaaggt
    28981 cacacgattt aagaaattcc agatttgcct ggcggcgcgc acgcacatca cgaatggcgt
    29041 gtttactggc ataaggagaa cggtctttat tgacctcccc gacagctatc agtaacgcct
    29101 catgccagcg catacgctgg cctttaagct gatgagtcca ccactcatcg ttaaacaggc
    29161 gggcaatggc agaatatgcc tgcctcgtgg tcatctgtcc tttacggtat tttttccagt
    29221 agagcgggga aatattgaaa gcacgtgcag cgccagcaac atgaccatac aggtgagcct
    29281 gcgcctcatc cgtaaacagc gattcttttt caccatgcgc atcaacccag gcatcgcaga
    29341 gttcctcata cattatgaaa agctgcgatg agatacgggc ggcaaacttt ttcagctcct
    29401 tgtcattcat tccaggcagg cgcgcatagt ggtcacgctc tgccagaaac agcaacgacg
    29461 cgtcggtgtt catttcatgg cgctgattca cacgctcaat gcgcggccat aaacgacgct
    29521 gaaaagtgga tgtgaggaaa taaaacccgt gcaccgggct tttattgcgc cggatgtagt
    29581 catagcgtga agtaaacagc gagcgcaaaa agtaaggcag gcggttaatc gtggataaaa
    29641 caccttgcac ctgacgcatc tcatcacgtg taaggggtct ttcgcgcccg acggcctcgc
    29701 gtggcgcgtt ccatgcataa gcaccggtaa acatcttacc ggtgcctgcg gcaaatgctg
    29761 acggagggac aaaacgcccg gaggctttaa cggccatatg agccaaaagc ctctgaacaa
    29821 cgcctgctga gttgctcaac ctgcgcgttt aaatcagcaa aagactttgc gcttccggtc
    29881 agaatatcgt gatgcatcag gccggaaacg agctggctta atttcgggta ataaccaacc
    29941 accgccagcc attcctgacc ggcgttttta ccgctttcag ctctcttttt ctcgtggaga
    30001 ataaactgaa agctgtcact ggtaacgaca taacgttcgc caatttcaat acgaatactc
    30061 atgccgttct ccggtaatgt ttgttttttg cttcaaagac tgactgacag gaaacacaac
    30121 gcgtggctga cggataagcc gcacgacggg cagcaggtat tggcgcgtca cactcttcgc
    30181 aaaccagcgc agaagcaccg caatgtttta cccttgccgc gttaatctgg cgctccagta
    30241 attcagcctg ttgttcctga ataaaatcta cgttgtccgg cattaccagt tccttttgtc
    30301 gttaaggttt ttaaattcat cagcgcaata gctggcgatt tctgtcgtta atttcgtcag
    30361 ttcatccacg gaggagattt gcttgtgaaa cacagcgcgt ttaacaagta aattgaccac
    30421 atcagacagg aggtttaatt cgttctgata aatcgcgata acagactcag ttatttcgcg
    30481 tttttcttta tcaagaccaa gttgaataag agacaaatcg ccatttttca taacggcgat
    30541 ttttagggcg ttattcagta atacaactga acgagaacag gacatcaaag cacctccccg
    30601 cgagacaatc cgatattgtg aaatttttcc gactcctgac tgagcagctc gactatctcc
    30661 acgcgggata actccgcctt tgtgatgtgg cgaatcatgg cgtcaagatg agaagaaaag
    30721 cgcgtcgctg cgtcggcctg tgcttcggtt ctggcctgtt gcagcagtaa tgcgtattta
    30781 ccgcactgat tttcagaaac tgtatgcatg actttctcca ggcaaaaaga agccccgcac
    30841 gattaagtgc gttaaaaact ctggttaatt acttaatgca gatattgctc tggttttacc
    30901 gacgtcagaa ttgtcggtgc atactcaaac aggctgaata attcacgtaa tgcacggaat
    30961 aaagcatcac gccagtaaca tgattcttca ttaattcgcc agtatggctg gttgaattct
    31021 ttttcagtca gtcgtgcgtg cataaataaa gtgcgacgct gactgactgt taaaaaacta
    31081 atatatgcat actcacttgc gccaacctga cggcgttttg agaatgcgcc acgcaattca
    31141 tcaattgcac aaaccagtcg ttcacgttcg acgtcgttca tttcttcaaa acgcatcgtt
    31201 gcgtgacgct gttttaactg tgcatgaaag caaaccgtta gccgttcgcg ctccatcatc
    31261 tgattataat aatcacatgt atcctgccag cgagggacgg caagatgctt gccaattatc
    31321 cggcgcatag ctgctggctg tttttcaacg agattaagcg tcatcactgt catttccagc
    31381 ccctccggct tttcagaaag gtcagagcct tttttaacgg actctgtttt ttggtgcgga
    31441 taatgattcc cttacgcccc ttaccgtggg tgatggtgaa gtcaatcgcc ctggggcttt
    31501 cgttacgcag taactgagca atacaacgcg gttcactcat aatcacaacc ccatccacaa
    31561 aagccatgca tcacgctgtt caactggtcg gttataaaac gcctctcgta cagcgcgatt
    31621 aaactctgga atgaaaaccc acttctcacc gacacgagcg ttcggcttac ttggatcacg
    31681 aagctcaata actggcaact tattctcttt taccatcttg actacagccg tttctggctt
    31741 accaagtaac tctgcaaact taaccgtatg taccgcatca atcgggtact gaatcacata
    31801 gtcattgact tccattgatt agcccttttt gctttcgtgt tacccttatt agatccagtc
    31861 ccttctaggt cgcacctgtc ctttctaggg actggctaac acactcaaaa ggtcaccaat
    31921 acacaacctt ttgacgggaa tataagtcac caataggtta ctgtcaaatg cagacattcg
    31981 aaaaactgaa agcgattagg aaagcagaag gcttaacaca ggcgaaattc agcgaaatta
    32041 gcgggatagc tctaggaaca gtcaaaaatt acgaaagtgg gcataaagac cctggtctca
    32101 gcatcgttat gcgagtcaca aatacgcctt tatttaaaaa atatacgctc tggttaatga
    32161 ctggtgatac gtcaccacaa gctggtcaga tcgcgccggc tctcgcacac attgggcaaa
    32221 aaccaacaga atcagaccac tccgaaaaac agactggtta acactctata aacattacat
    32281 tttcaccatt tgttaccaag atggtgaata cagcgtcaga gggctttctt atgtcaatta
    32341 agaagctcga tgatggacgc tatgaagtgg acattagacc tcgcggtcgc gacggaaaac
    32401 gcatccgcag gaaatttgaa agaaaagctg aggctgtagc atttgagcga tacacaatcg
    32461 cctacgccag ccagaaagaa tgggcaggtc agcgagcaga tcgcagaact ttgagtgagt
    32521 tgctgaacat ctggtggaaa tatcacgggc aaaaccacga gcatggaaca aaagagttta
    32581 atcatctgct caaaaccatc agcggcatag gtgatatacc agtgagccgg atgagcaaaa
    32641 gagctttgat ggattatcgt tccatgcgac tacgtgatgg tatcagtgcc gcaacgataa
    32701 accgtgacat gtaccgatta tccggcatgt tcacaaaatt aattcaattg gatgaatttt
    32761 ccgggcaaca cccaattcac ggactgccgc cactggcgga ggccaaccct gaaatgacgt
    32821 tcctggaaaa agcagaaatc gaaaaactgt taaatgtttt ggatggtgat gacttacttg
    32881 tcgcactttt atgtctgagc actggaggaa gatggacgga agttgccacg ctaaaaccag
    32941 cacagattac aaattgcagg gttaccttcc tgaaaaccaa aaacggtaaa aagcgaaccg
    33001 tgccgatttc tgaggaactg gagaaaaaag ttaaagagga ggccagcgct aaattattca
    33061 aagttgatta tgagaagttt tgcgggattt tacgcagagt gaagccagat ataccaccca
    33121 atcaggcaac ccacatcctg cggcatacat tcgcaagcca tttcatgatg aatgggggca
    33181 atataatcgc actgcaacag attctgggac atgcgagcat tcagcagacg atggcctatg
    33241 cgcaccttgc gcctgactac ctgcaaaatg ccgtcgcgct gaatcctcta aaaggcggag
    33301 tgacgttata aatttccctt ctgagtgtcc acatagtgtc cacactctca gaactttgta
    33361 gcccttccag tcccttatag gttttcttaa gttactgttt tcttacggaa accgatgtaa
    33421 gtgattgata aaaaaaaccc ccacatcatg tgggggaaga cagggatggt gtctatggca
    33481 aggaaaacag ggtttactac tgggaacgtg agttgctact actcaatagc ttcaacgatg
    33541 aacttttttg ccattgcgtc acgtcgcgca actgctccat tcgttgttga tgtttctcgt
    33601 ttaaaaccgc ttgctgctcc ggcgttaaca ggcgatacat ttggttgcgg acttttgcca
    33661 tctcaacctg acgagcaatt tgctcattcg ccattttttc tgcctgtgcg cgcacagcgt
    33721 tttcatcaaa attttctgcg gtgacaaggc gatgcattgt ctccagttcg ctaacattaa
    33781 caggaggctg ttcgtgccgg gcctgttgca taagatctcg catctgctga cgctgatgtt
    33841 cggttaaact tatgccgtcg aacatatggc tctgcgtact gcgctgcgta agttcttcac
    33901 ccggatgcca gttatcgcct gaaccgactt cagcagcgtg gcttaatgaa ctgactgcca
    33961 gcgttgaggc catgacggca gcggtaacta tgcgcatcat ttgctcccaa aatctttctg
    34021 tcgcgattca acgatagaga gtttacgatt caggctgcaa acatgcgtca gggggtgtaa
    34081 aacaacgtaa agtcatggat tagcgacgtc tgatgacgta atttctgcct cggaggtatt
    34141 taaacaatga ataaaatcct gttagttgat gatgaccgag agctgacttc cctattaaag
    34201 gagctgctcg agatggaagg cttcaacgtg attgttgccc acgatgggga acaggcgctt
    34261 gatcttctgg acgacagcat tgatttactt ttgcttgatg taatgatgcc gaagaaaaat
    34321 ggtatcgaca cattaaaagc acttcgccag acacaccaga cgcctgtcat tatgttgacg
    34381 gcgcgcggca gtgaacttga tcgcgttctc ggccttgagc tgggcgcaga tgactatctc
    34441 ccgaaaccgt ttaatgatcg tgagctggtg gcacgtattc gcgcgatcct gcgccgttcg
    34501 cactggagcg agcaacagca aaacaacgac aacggttcac cgacactgga agttgatgcc
    34561 ttagtgctga atccaggccg tcaggaagcc agcttcgacg ggcaaacgct ggagttaacc
    34621 ggcactgagt ttaccctgct ctatttgctg gcacagcatc tgggtcaggt ggtttcccgt
    34681 gagcatttaa gccaggaagt gctgggcaaa cgcctgacgc ctttcgaccg cgctattgat
    34741 atgcacattt ccaacctgcg tcgtaaactg ccggatcgta aagatggtca tccgtggttt
    34801 aaaaccttgc gtggtcgcgg ctatctgatg gtttctgctt catgataggc agcttaaccg
    34861 cgcgcatctt cgccatcttc tggctgacgc tggcgctggt gttgatgttg gttttgatgt
    34921 tacccaagct cgattcacgc cagatgaccg agcttctgga tagcgaacag cgtcagggtc
    34981 tgatgattga gcagcatgtc gaagcggagc tggcgaacga tccgcccaac gatttaatgt
    35041 ggtggcggcg tctgttccgg gcgattgata agtgggcacc gccaggacag cgtttgttat
    35101 tggtgaccac cgaaggccgc gtgatcggcg ctgaacgcag cgaaatgcag atcattcgta
    35161 actttattgg tcaggccgat aacgccgatc atccgcagaa gaaaaagtat ggccgcgtgg
    35221 aactggtcgg tccgttctcc gtgcgtgatg gcgaagataa ttaccaactt tatctgattc
    35281 gtccggccag cagttctcaa tccgatttca ttaacttact gtttgaccgc ccgctattac
    35341 tgctgattgt caccatgttg gtcagtacgc cgctgctgtt gtggttggcc tggagtctgg
    35401 caaaaccggc gcgtaagctg aaaaacgctg ccgatgaagt tgcccaggga aacttacgcc
    35461 agcacccgga acttgaagcg gggccacagg aattccttgc cgctggtgcc agttttaacc
    35521 agatggtcac cgcgctggag cgcatgatga cctctcagca gcgtctgctt tctgatatct
    35581 ctcacgagct gcgcaccccg ctgacgcgtc tgcaactggg tacggcgtta ctgcgccgtc
    35641 gtagcggtga aagcaaagaa ctggagcgta ttgaaaccga agcgcaacgt ctggacagca
    35701 tgattaacga cctgttggtg atgtcacgta atcagcagaa aaacgcactg gttagcgaga
    35761 ccatcaaagc caatcagttg tggagtgaag tgctggataa cgcggcgttc gaagccgagc
    35821 aaatgggcaa gtcgttgaca gttaacttcc cgcctgggcc gtggccgctg tacggcaatc
    35881 cgaacgccct ggaaagtgcg ctggaaaaca ttgttcgtaa tgccctgcgt tattcccata
    35941 cgaagattga agtgggcttt gcggtagata aagacggtat caccattacg gtggacgacg
    36001 atggtcctgg tgttagcccg gaagatcgcg aacagatttt ccgtccgttc tatcgtaccg
    36061 atgaagcacg cgatcgtgaa tctggcggta caggtttggg gctggcgatt gttgaaaccg
    36121 ccatccagca gcaccgtggc tgggttaaag cggaagacag tccgcttggc ggtttacggc
    36181 tggtgatttg gttgccgctg tataagcgga gttaaactcc gcattgtagg caggataagg
    36241 cgtttacgcc gcatccggca tttgagcagg atgcctgatg cgacgctgat agcgtcttat
    36301 caggcctaca ctccgggggt tattttcccc acaaccggcg ggaaaaatct tcgatcttgc
    36361 cgctcagtcg gcgcttttgc atcgtccgcg tccagctttt cgataacccg gcggcggaga
    36421 gtaaacggtg atactgatcg tcatcaaacg gcatatgcca tgcgatggcg gcagcctcct
    36481 gcacggtcac atcactgaca cgggaaacca actccagtgg cgcatcagca gataccaacc
    36541 ctggtgcaat tacgctatac aaccacccca ctttacccgt gttttgcatc aactgcgcta
    36601 tgtcgctgat atcaaaatgg taattgagtt tatagcaagg cgagcgcggc tggctgacct
    36661 gaattaatgc ctctccccag cggaaaatat cgcccatata aacattactt tccgtcagac
    36721 cgtcggttga gagattttca ccaaacgcag gcgcaacaaa caactccgcc tgttccggaa
    36781 attcccgcgc ccagtagaga tagtgctcac gaggataatg acaaagcgct ctgtctggcc
    36841 cgccgtgaac cattttctcc gcctgctcgt caccttccag ccccagctcc gtcagcatca
    36901 actcaccatc aacctggatt ttggcaattg cgctgggttt gccttcggga taagcctgaa
    36961 tcttgcctgt gtatacatca accggatatc gcatctgttc cctctccacg agaaatttcc
    37021 ccattagacc atatttattt tatgtcaggc gtgctgtttt cgcggtatgt atccaggcag
    37081 gagctcaata gcaaaccgga cgaacgttag ttcatccggc tgtggggatt aaggaagcag
    37141 gtttatttca ccgtacctaa aatttcgatt ttcgccgctc tggctttgac tttacgtgac
    37201 cagatagagg taagaattgg caccagaatc gaagtcacaa tgaccgccgt cgctaccagc
    37261 gaagttgctg ccggagccat cggtttaaac gcaggcacca tttctgcaat cagcacaggt
    37321 gtcgctaccg cggcccctgc ggaactggaa gcggcaattc cggcagtgcc atcgccaccg
    37381 ccaatcaatt tatcagcgat aatcaacgga ataccggtca cgataattac tgccacaccc
    37441 aacaggatcc ccagcaaacc tgtctgggca attacagtca aatcaatggt attgcccagc
    37501 gcgaaggcaa agaatggaat cagcgtctgc accgctttgc tgaaaaattc tcgtaattca
    37561 gggtcaaggt tcccaagggc aaagcccacc aggaacggta atactgcgcc gacgaaaaca
    37621 tgcggttcaa acgaggcaat cccggcagtg cccagaataa tcatcgtcat gagcggcccg
    37681 gactccaatg acatcaacac aaatgcccca gcttcttctt ttgtgccgta ctgctgcatg
    37741 atggaagcgt aaagtccgcc gttggtcata tccatcgccg ccaccagcgc cagcgttgaa
    37801 agtccggcaa agaatccaac ttcaacacca tgttccggaa taatgcgcga ggcaatcgcc
    37861 gcaaccaccc acgcgacggc aatttttgtt accaccagcg tgccggattt acgcaatacc
    37921 gttcccgtcg cgcttaattt tattgacgcc cccatgcaaa aaaaccacac cgccagaatg
    37981 ggcaccgtac cggtaatcat cccgttggtg aatgatccaa aatatttccc cgcccccggc
    38041 gagaaggtgt ggcacagtgc gccaaggaat agcgggacga gcatcatccc ccccgggatt
    38101 ttctcaatcg agcgttttat ctgcatttcc atcacctgtc ggcatatttt taagggctga
    38161 tgcgtcattc ctcaacggag catcagtgtt gatggaaacg aagatagaca ttcatttgat
    38221 caataaaagt gatcaaaacc acaccatttg aaacactgtt ttatttttat cggatcattg
    38281 tttttatttt gaaattgatc acaaaaaaac caccgccgtt ggcgatggtt cattttttaa
    38341 gctgcaatgc ggcgtaaacg cctcattgca gcaggcagca aatgattatt ttttcgccgc
    38401 gaaacgtgcc gctgcttcgt cccagttcac cacgttccag aactctttga tgtagtccgg
    38461 acggcggttc tggaatttca ggtagtaagc atgttcccac acatccaggc ccacaatcgg
    38521 gaagccggaa gcgccagaaa tagcttcacc catcagcggg gagtcctggt tagcagtaga
    38581 aaccaccgcc agtttatcgc ctttcagcac cagccatgcc cagccggaac caaagcggga
    38641 agctgccgct ttctcaaatt ctgctttgaa gttatcaacg gagccgaagt cacgttcgat
    38701 agccgctttc aggtcaccct gtaaggtggt gccttttttc agacctttcc agaacaggct
    38761 gtggttagcg tgaccgccag cgttgttgcg cagtacggtt ttcttgtctg ctggcagctg
    38821 gtccagtttg gtgatcagct cttcaaccgg caggttagcg aattctggca ggctttccag
    38881 cgccgcgttg gcgttgttga cgtaggtctg atggtgtttg gtgtggtgga tttccatggt
    38941 ctgcttatcg aagtgcggtt ccagggcatc gtaagcatac ggcagggatg gcagggtata
    39001 gctcatattc atctccagta ttgtcgggcg gccgattgtt aatgccgcgt aagcagttgg
    39061 ttcattatag ttaattaaat gatattgaaa atgattatca atgccgtact tttcgtaagg
    39121 gtatggtttt gcaggaaaat gcccaagatg tgaagcaaat cacccactta atgccgtgat
    39181 tgccagtaaa tcggcagcgg cggcaacagg cgaaaggtta atcgacagca cgatttttac
    39241 actcatctcg tcggagatgt gacgcgacga aaaatgatga ggataagaag atgagtaacg
    39301 cgattacgat ggggatattt tggcatttga tcggcgcggc cagtgcagcc tgtttttacg
    39361 ctccgttcaa aaaagtaaaa aaatggtcat gggaaaccat gtggtcagtt ggtgggattg
    39421 tttcgtggat tattctgccg tgggccatca gcgccctgtt actgccgaat ttctgggcgt
    39481 attacagctc gtttagtctc tctacgctgc tgcctgtttt tctgttcggc gctatgtggg
    39541 ggatcggtaa tatcaactac ggcctgacca tgcgttatct cggcatgtcg atgggaattg
    39601 gcatcgccat tggcattacg ttgattgtcg gtacgctgat gacgccaatt atcaacggca
    39661 atttcgatgt tctgattaat accgaaggcg gacgcatgac gttgctcggc gttctggtgg
    39721 cgctgattgg cgtagggatt gtgactcgcg ccgggcagtt gaaagagcgc aagatgggca
    39781 ttaaagccga agagttcaac ctgaaaaaag ggctggtgct ggcggtgatg tgcggcattt
    39841 tctctgccgg gatgtccttt gcgatgaacg ccgcaaaacc gatgcatgaa gccgctgctg
    39901 cactgggcgt cgatccactg tatgtcgctc tgccaagcta tgttgtcatc atgggaggcg
    39961 gcgcgatcat caacctcggt ttctgcttca ttcgtctggc aaaagtgaag gatttgtcgc
    40021 taaaagccga cttttcgctg gcaaaaccgc taatcattca caacgtgtta ctctcggcac
    40081 tgggcggttt gatgtggtat ctgcaattct ttttctatgc ctggggccac gcccgcattc
    40141 cggcgcagta tgactacatc agctggatgc tgcatatgag cttctatgta ttgtgcggcg
    40201 gtatcgtcgg gctggtgctg aaagagtgga acaatgcagg ccgccgtccg gtaacggtgt
    40261 tgagcctcgg ttgtgtggtg attattgtcg ccgccaacat cgtcggcatg ggcatggcga
    40321 attaatcttt ctgcgaattg agatgacgcc actggctggg cgtcatcccg gtttcccggg
    40381 taaacaccac cgaaaaatag ttactatctt caaagccaca ttcggttgaa atatcactga
    40441 ttaacaggcg gctatgctgg agaagatatt gcgcatgaca cactctgacc tgccgcagat
    40501 attgattgat ggtcattcca gtctgctggc gaaattgctg acgcaaaacg cgctcactgc
    40561 acgatgcctc atcacaaaac tcatccagcg caaagggact tttcaggctg gccgccagcc
    40621 gggtaatcag cttatccagc aacgtttcgc tggatgttgg cggcaacgaa tcgctggtgt
    40681 aacgatggcg attcagcgac atcaccaact gcccgaacag caactctgcc atttcgttag
    40741 caaacggcac atgctgacta ctttcatgct caagctgacc gataatctgc cgcgcctgtg
    40801 ccatccccat gctacctaag cgccagtgtg gttgccctgc gctggcgcta aatcccggaa
    40861 tcgccccctg ccagtcaaga ttcagcttca gccgctccgg gcaataaata atattctgca
    40921 aaaccagatc gttaacggaa gcgtaggagt gtttatcgtc agcatgaatg taaaagagat
    40981 cgccacgggt aatgcgataa gggcgatcgt tgagtacatg cagaccatta ccgcgccaga
    41041 caattaccag ctcacaaaaa tcatgtgtat gttcagcaaa gacatcttgc ggataacggt
    41101 cagccacagc gactgcctgc tggtcgctgg caaaaaaatc atctttgaga agttttaact
    41161 gatgcgccac cgtggctacc tcggtcagag aacgaagttg attattcgca atatggcgta
    41221 caaatacgtt gagaagattc gcgttattgc agaaagccat cccgtccctg acgaatatca
    41281 cgcggtgacc agttaaactc tcggcgaaaa agcgtcgaaa agtggttact gtcgctaaat
    41341 ccgcagcgat aggcgatgtc agtaacgctg gcctcactgt ggcgtagcag atgtcgggcc
    41401 ttcatcagtc gcaggcggtt caggtaacgc tgaggcgtca gtcccgtttg ctgcttaagc
    41461 tgccgatgta gcgtacgcag cgaaagagaa aattgatccg ccacggcatc ccaattcacc
    41521 tcatcggcaa aatggtcctc cagccaggcc agaagcaagt tgagacgtga cgcgctgttt
    41581 tccaggttct cctgcaaact gcttttacgc agcaagagca gtaattgcat aaataagatc
    41641 tcgcgactgg cggtcgaggg taaatcattt tcctcttcct gctgttccat ctgtgcaacc
    41701 agctgtcgca cctgctgcaa cacgctgtgg ttaacgcgcc agtgagacgg atactgccca
    41761 tcctgctctt gtggcagcaa ctgattcagc ccggcgagaa actgaaatcg atccggcgag
    41821 cgatacagca cattggtcag acacagatta tcggtatgtt catacagatg ccgatcatga
    41881 tcgcgtacga aacagaccgt ccccccggtg atggtatagg gctgcccatt aaacacatga
    41941 atacccgtgc catgttcgac aatcacaatt tcatgaaaat catgatgatg ttcaggaaaa
    42001 tccgcctgcg ggagccgggg ttctatcgcc acggacgcgt taccagacgg aaaaaaatcc
    42061 acactatgta atacggtcat actggcctcc tgatgtcgtc aacacggcga aatagtaatc
    42121 acgaggtcag gttcttacct taaattttcg acggaaaacc acgtaaaaaa cgtcgatttt
    42181 tcaagataca gcgtgaattt tcaggaaatg cggtaagcat cacatcacca caattcagca
    42241 aattgtgaac atcatcacgt tcatctttcc ctggttgcca atggcccatt ttcctgtcag
    42301 taacgagaag gtcgcgaatt caggcgcttt ttagactggt cgtaatgaaa ttcagcagga
    42361 tcacattatg acctttcgca attgtgtcgc cgtcgatctc ggcgcatcca gtgggcgcgt
    42421 gatgctggcg cgttacgagc gtgaatgccg cagcctgacg ctgcgcgaaa tctatcgttt
    42481 taacaatggc ctgcatagcc agaacggtta tgtcacctgg gatgtggata gcctggaaag
    42541 tgccattcgc cttggattaa acaaggtgtg cgaggaaggg attcgtatcg atagcattgg
    42601 gattgatacc tggggcgtgg actttgtgct gctcgaccta cagggtaagc gtgtgggtct
    42661 gcccgttgct tatcgcgata gccgcaccaa tggcctgatg acgcaggcac aacagcaact
    42721 cggcaaacgc gatatttatc agcgtagcgg catccagttt ctgcccttca atacgcttta
    42781 tcagttgcgt gcgctgacgg agcaacaacc tgagcttatt ccacacattg ctcacgctct
    42841 gctgatgccg gattacttca gttatcgcct gaccggcaag atgaactggg agtacaccaa
    42901 cgccacgacc acgcaactgg tcaatattaa tagcgacgac tgggacgagt cgctactggc
    42961 gtggagcggg gccaacaaag cctggtttgg tcgcccgacg catccgggta atgtcattgg
    43021 gcactggatt tgcccgcagg gtaatgagat tccggtggtc gccgttgcca gccatgatac
    43081 cgccagcgcg gttatcgcct cgccgttaaa cggttcacgc gccgcttatc tctcttctgg
    43141 cacctggtca ttgatgggct tcgaaagcca gacaccattt accaatgaca cggcgctggc
    43201 agccaacatc accaatgaag gcggggcgga aggtcgctat cgggtgctga aaaatattat
    43261 gggcttatgg ctgcttcagc gggtgcttca ggagcggcaa atcaacgatc ttccggcgct
    43321 tatcgccgcg acacaggcac tgccggcttg ccgcttcacc atcaatccca atgacgatcg
    43381 ctttattaat cctgacgaga tgtgcagcga aattcaggct gcgtgtcggg aaacggcgca
    43441 accaatcccg gaaagtgatg ctgaactggc gcgctgtatt gtcgacagtc tggcgctgct
    43501 gtatgccgat gtgttgcatg agctggcgca actgcgcggt gaagatttct cacaactgca
    43561 tattgtcggc ggtggctgcc agaacgcgct gctcaaccag ttatgtgctg atgcctgcgg
    43621 tattcgggtg atcgccgggc ctgttgaagc ctcgacgctc ggcaatatcg gcatccagtt
    43681 aatgacgctg gatgaactca acaatgtgga tgatttccgt caggtcgtca gcaccaccgc
    43741 gaatctgacc acctttaccc ctaatcctga cagtgaaatt gcccactatg tggcgcagat
    43801 tcactctaca cgacagacaa aggagctttg tgcatgacca ctcaactgga acaggcctgg
    43861 gaactggcga aacagcgttt cgcggcggtg gggattgatg tcgaggaggc gctgcgccaa
    43921 cttgatcgtt tacccgtttc aatgcactgc tggcagggcg atgatgtttc cggttttgaa
    43981 aacccggaag gttcgctgac cggtgggatt caggctactg gtaattatcc gggcaaagcg
    44041 cgtaatgcca gcgagctacg tgccgatctg gaacaagcta tgcggctgat tccgggtccg
    44101 aaacggctta atttacatgc catctatctg gaatcagaca cgccagtctc gcgcgaccaa
    44161 atcaaaccag agcacttcaa aaactgggtt gaatgggcga aagccaatca gctcggtctg
    44221 gattttaacc cctcctgttt ttcgcatccg ctaagcgccg atggctttac gctttcccat
    44281 gccgacgaca gtattcgcca gttctggatt gatcactgca aagccagccg ccgtgtttca
    44341 gcctattttg gcgagcaact tggcacaccg tcggtgatga acatctggat cccggatggc
    44401 atgaaagata tcaccgttga ccgtctcgcc ccacgtcagc gtctgctggc agcactggat
    44461 gaggtgatca gcgagaagct ggatccggcg caccatatcg acgccgttga gagcaaattg
    44521 tttggcattg gcgcggagag ctacacggtt ggctccaatg agttttacat ggggtatgcc
    44581 accagccgcc agacggcgct gtgcctggac gccgggcatt tccacccgac tgaagtgatt
    44641 tccgacaaga tttccgccgc catgctgtat gtgccgcagt tgctgctgca cgtcagccgt
    44701 ccggttcgct gggacagcga tcacgtagtg ctgctggatg atgaaaccca ggcgattgcc
    44761 agtgagattg ttcgtcacga tctgtttgac cgggtacata tcggcctgga tttctttgat
    44821 gcctctatca accgcattgc cgcatgggtc attggtacgc gcaatatgaa aaaagccctg
    44881 ctacgtgcgt tgctggaacc taccactgaa ctgcgcaagc tggaagcagc gggcgattac
    44941 accgcacgac tggcactgct ggaagaacaa aaatcattgc cgtggcaggc ggtctgggaa
    45001 atgtattgcc aacgtcacga taccccagtg ggtagcgaat ggctggaaag cgtgcgggct
    45061 tatgagaaag cgattttgag ccagcgtggg taaacactgc cggatgcggc gcgagcgcct
    45121 tatccggcct acgggtcgtg catccgacaa caccgaattt acaggaacac agaacatgca
    45181 aaacattacc cagtcctggt ttgtccaggg aatgatcaaa gccaccaccg acgcctggct
    45241 gaaaggctgg gacgagcgca atggtggtaa cctgacgctg cgcctggatg acgccgatat
    45301 cgcaccgtat cacgacaact ttcatgcaca accgcgctat atcccgctca gccagcccat
    45361 gcctttactg gcaaatacac cgtttattgt caccggctcc ggcaaattct tccgtaacgt
    45421 ccagcttgat cctgcggcta acttaggcgt cgtaaaagtc gacagcgacg gcgcgggcta
    45481 ccatattctc tgggggttaa ccaacgaagc cgtcccgaca tccgaacttc cggcgcactt
    45541 cctctctcat tgcgaacgca ttaaagccac caacggtaaa gatcgggtga tcatgcactg
    45601 ccatgccacc aacctgatcg cccttaccta cgtattggaa aacgacaccg cggtcttcac
    45661 tcgccagctt tgggaaggca gcaccgagtg tctggtagtg ttcccggatg gcgtcggcat
    45721 tttgccgtgg atggtgcccg gcaccgacga aatcggccag gcgaccgcac aggaaatgca
    45781 aaaacattcg ctggtgttgt ggcccttcca cggcgtcttc ggcagcggtc cgacactgga
    45841 tgaaacattc ggtttaatcg acaccgcaga aaaatcagca caagtattag tgaaggttta
    45901 ttcgatgggc ggcatgaaac agaccatcag tcgtgaagag ctgatagcac tcggccagcg
    45961 tttcggcgtt acaccacttg ccagtgcgct ggcgctgtaa ggagcaaata tgatccgcaa
    46021 agcctttgtc atgcaggtta accccgacgc ccacgaagag tatcagcgtc ggcataatcc
    46081 aatctggccg gaactggaag cagtgttgaa atctcacggt gcgcataact acgccattta
    46141 cctcgacaaa gcgcgtaatc tgctgtttgc cacggtagag attgaatctg aagaacggtg
    46201 gaatgcggta gccagtactg atgtttgcca acgttggtgg aagtatatga ccgacgttat
    46261 gccagctaac ccggataaca gcccagtcag tagcgagctg caagaagtgt tttacctgcc
    46321 ttaatgagtt gcggtgccgg aaaatatcca gcaccgcgcc ttcatcagaa gaagtattta
    46381 aagcgaacgc gtgcgccgta acggtcatca gtatcatcac cgtgtttttt atcaccgtcg
    46441 ctatccagaa tggagtaata cgtcccgagg taaatattaa agttctccat atccatcaca
    46501 ttagcgaact gataagacgc atgaatggtg tgaatggtgt acgtcccttc atcatcaatc
    46561 cagcagtcgt tatcacaaac tacaccgctg aattcatcaa tcttattgtg cgcgtagata
    46621 taacccagct cgaaacgttt ccacagcgcg ttaatcccgg cagtgaaatc tttctcatta
    46681 ttagcatcta aataggcggt attaagatta accacgacgc cattttccgg atccgttttc
    46741 aggccattcc aggtcatgct cataccatag ccggtacgat cggactgatc gacaaaatta
    46801 cccttgctat cggtataacc ataagcatta tttactacgt tcgcttccat cgctgcggaa
    46861 acggtgaatt cttccgtcgg cgaccaggca attaccggac gcagataagc aacatttttc
    46921 tgctgctcca tatcgcgtcc atgataatta ccgtcattat ataaagatgt tccgtcttca
    46981 agtaacgtgt ttaactcaaa ataccagtta tcaagttgtt tgctgacgag gaaattaccg
    47041 ccagcgttag agcgcccgcg gccctctttc atcatataga tataaccgct gccatcgtca
    47101 taaagatcgt tcgcagtatt accggaatgt tcaacaaagg tatcctgatt cagcgggaac
    47161 atatcgtagg cttcaaaacg gccgactttg accttccagt cattctcttt cccaaagaag
    47221 aataccgcat catcgatatt tactgaaccg tgcatgtccc ccagcggttg tgcggagaac
    47281 ccggcgaaat agccattatc cagttttcgc ataccatcaa aacctaacaa aatacgacca
    47341 ttgagattcc actgttcatt agtgggatcg ctattcacat cagcgttggt cattgccagc
    47401 aggccatgat tactttctgc atccatatta aattctacgt cgccgtagat ttttaaatcg
    47461 ccataacctg agagcaaaag attaggtgag gtggagtttt tctttgcaga ttccgccgtt
    47521 gcctgtccat ttaccggcgt attgccctgg gcagccttaa tttcgcggat ctcagcggct
    47581 tgttgtgtct gcagctgttt aacctggctc tctgcttttg ctgcccgcat ttctgccgct
    47641 tccaggcgtt cttccagcaa tgccatgcgt tgttccagcg ttaaatgctg tgtttttgcc
    47701 attccggtac tacatgccaa taataaaccg atggctaaag ataatgtcga ttttttcatt
    47761 atttacgttc cctgaaataa aagagaccct acgctttcca aaaggaatac gtatatagag
    47821 aaatataagg tgtgcacttt cgaattttta atccaacttc agcaggccga tggatagtat
    47881 cccgctgcat aatatgagta aatccgctga tttcctggaa cgtgaggaat ataacaatag
    47941 tgcctttttt aaccaaatat cgtaaaaaat attagatata attcacgctg actcataagc
    48001 cacaaatata aataaccttg ctgcaaaaca tgtgcgacag ttaaacattc acaacatcat
    48061 taaataatga ttttttattc actgaattta tttttcccat caatatgtgc cttacaccac
    48121 aaaatttatc cttcactctg ccttatattg tcacgacttc acgacatatc cgcgctcatc
    48181 acaaaaccgc cccgccgtta caattaacta taatattttt tcatggagtc caataatggc
    48241 agcacttact gcaagctgta ttgacctgaa tattcagggc aatggcgctt attccgttct
    48301 gaagcagttg gcgacaatgg cgttacaaaa cggttttatc accgactcac accagttcct
    48361 gcaaaccctg ctgctgcgcg aaaaaatgca ctccacggga tttggttccg gcatcgccgt
    48421 gccgcacggt aaaagcgcct gcgttaaaca accgttcgtg ttattcgccc gcaaaacgca
    48481 ggccattgac tggcaggcca gcgatggcga agacgtcaat tgctggatct gcctcggcgt
    48541 gccacaaagt ggcgaagagg aacaggtcaa aatcatcggc acgctgtgcc gcaaaattat
    48601 ccaccaggat tttatcaacc aactgcgaca gggcgacgcc gaccaggtac ttgctttgtt
    48661 aaatcaaacc ctcagctcat aaggaagtgg cgatggagtc atccttacgt attgtcgcga
    48721 tcaccaactg ccccgccggg atcgctcaca cctacatggt ggcggaagcc ctggaacaga
    48781 aagcccgttc tctcggtcat accattaaag ttgaaactca aggatcaagc ggcatcgaaa
    48841 accgcttatc cagcgaagag attgccgctg ccgattacgt gattctcgct accgggcgtg
    48901 gcctgagcgg tgatgatcgc gcgcgatttg ccgggaaaaa ggtttatgag attgccatct
    48961 cccaggcgct gaaaaatatc gaccagattt tcagtgaatt accgacaaac tcgcagcttt
    49021 ttgccgcaga tagcggcgtg aagctcggca aacaggaagt gcaaagtggc agcgtaatga
    49081 gccatctgat ggcgggtgtt tctgcggcgc tgccgtttgt catcggtggc ggtatcctgg
    49141 tggcgcttgc caacatgctg gtgcagttcg gtttgccgta taccgatatg tcgaaaggcg
    49201 cgccgtcgtt tacctgggtg gtggaatcca tcggttatct cggcttcacc tttatgatcc
    49261 ccatcatggg cgcttatatt gcctcgtcga ttgccgataa accggctttc gctccggcgt
    49321 ttctggtttg ctatctggcg aatgacaaag ccctgctcgg cacccagtct ggcgcgggct
    49381 tcctcggtgc ggtggtgctg gggctggcga ttggctattt cgtcttctgg ttccgtaaag
    49441 tgcgccttgg caaagcatta caaccgctgc tcggttccat gctgatcccg ttcgttactc
    49501 tgctggtttt tggcgtactg acttactacg ttatcggacc ggtgatgtcc gacctcatgg
    49561 gcgggttgct gcacttcctg aacaccattc ctccgtcaat gaagtttgcg gcggcgtttc
    49621 tggttggtgc gatgctggca ttcgatatgg gcggccccat caacaaaacc gcctggttct
    49681 tctgcttctc tctgctggaa aaacacattt acgactggta cgccatcgtc ggcgttgtcg
    49741 cgctgatgcc gcctgtcgcg gcgggtcttg cgacgttcat tgcgcccaaa ctgtttactc
    49801 aacaggaaaa agaagccgcc agtagcgcca ttgtggtcgg tgccacggtc gcgaccgaac
    49861 cggctattcc ttacgctctg gcggcaccac tgccaatgat caccgccaat acgctggcgg
    49921 gcggcattac cggcgtgctg gtgatcgctt tcggaatcaa acgactggca cctggtttgg
    49981 gtatctttga cccgctgatc ggcctgatgt cgccggtagg ttcgttttac ctggtgctgg
    50041 cgattggcct cgcgctgaac atttcattca ttatcgtcct gaaaggatta tggttacgcc
    50101 gcaaagccaa agctgcgcaa caggagcttg tccatgaaca ttgagttgct gcaacagttg
    50161 tgcgaagcca gcgccgtcag cggcgatgaa caggaagttc gcgacattct gataaacacg
    50221 ctggaacctt gcgttaatga gatcaccttt gatggtctgg gcagctttgt tgcccgtaag
    50281 gggaataaag ggccaaaagt tgctgttgtc gggcatatgg atgaagtcgg ctttatggtc
    50341 acccacatcg acaagagcgg ttttctgcgt tttaccacca ttggcggctg gtggaatcag
    50401 tcgatgctca accaccgggt aaccatacgg acacacaagg gagtgaaaat ccctggtgta
    50461 attggctccg tcgccccaca tgcgttaacc gaaaagcaaa agcaacaacc gctgtcattt
    50521 gatgagatgt tcattgatat tggcgcgaac agtcgcgaag aggtggaaaa acgcggcgtg
    50581 gaaattggca attttattag cccggaagcc aattttgcct gctggggcga agataaagtg
    50641 gtcggcaagg cgttggataa ccgcatcggc tgcgcaatga tggccgagct actacagaca
    50701 gtaaataacc ccgaaattac gctttatggc gttggcagtg tggaagaaga agttgggcta
    50761 cgcggggcac aaacctcggc tgaacacatt aaaccggatg tggtgatcgt gctggatacc
    50821 gccgtcgcgg gcgatgttcc gggcattgat aacattaaat acccgctgaa actgggccag
    50881 gggccggggc tgatgctgtt tgacaagcgc tacttcccca accagaaact ggtggcggcg
    50941 ttaaaaaact gtgccgcaca taacgattta ccgctgcaat gttccaccat gaaaaccgga
    51001 gcgacggatg gcgggcgcta caacgtgatg ggcggcgggc gtccagttgt cgcgctgtgt
    51061 ctgccaacgc gttatctgca cgccaatagc ggtatgattt caaaagccga ttacgatgct
    51121 ctgctcacgt tgataaggga ttttctgacc accttaaccg cggagaaagt caacgcgttt
    51181 agccagttcc gtcaggtgga ttaatgctaa acgaacgcca gttaaagatt gtcgatctgc
    51241 tggagcaaca gccacgcacg cctggcgagc tggcgcaaca gactggcgtt tccggcagga
    51301 ccatcctgcg tgatattgac tatctcaact ttacccttaa cggcaaagcc cgcatttccg
    51361 ccagtggcaa cgcgggctat cagctggaaa tcttcgagcg ccgcagcttt tttcagttgc
    51421 tgcaaaagca cgataacgac gatcggctgc tggcgctgtt attactgaat accttcactc
    51481 cccgtgcgca actcgcctcg gcgcttaatt tgccagaaac gtgggtagca gagcgcctgc
    51541 cccgcctgaa acagcgctat gaacgcggtt tttgtctcgc tagtcgccct ggtttgggcc
    51601 atttcattga tgagacagaa gagaaacgca ttattttgct ggcgaatctt ttacgcaaag
    51661 atccgttttt aattccgctg tcgggcgtaa cacgagacaa ttttcaacaa ttaaccaccg
    51721 cctgcgaaaa gcaacgtcgc tggccgctga tgcaaggcga ctatctctca agcctgattc
    51781 tggcgattta cgccctgcgt aatcaactga ccgatgagtg gccgcaatat cccggtgacg
    51841 agataaaaca aatcgttgaa cagagcggcc tgtttcttgg tgataacgct gtaagaaccc
    51901 tgacaggttt gatagagaaa cagcatcagc aagcgcaggt aatttctgcc gatcatgtgc
    51961 tggggttgct gcaaagggtg ccaggcatcg cgtcattgaa tattattgat acgcagttgg
    52021 ttgagaatat taccgggcat ttattacgtt gccttgccgc accggtgtgg attgccgagc
    52081 accgccaaag cagcatgaat aacctgaaag ccgcctggcc tgcggccttt gatatgagtc
    52141 tgcactttat tacgctactg cgtgaacagc tcgatattcc ccttttcgac agcgatctga
    52201 tcggtttgta ttttgcctgt gcgctggaac ggcatcaaaa cgaacgccag ccgatcattt
    52261 tgctctcgga ccagaacgcg attgccacca ttaatcagct cgccattgag cgcgatgttt
    52321 tacattgtcg ggtgattatt gcccgtagct taagcgaact tgttgccatt cgcgaagaga
    52381 ttgagccgtt attgatcatt aacaacagcc attatttact ggatgacgcg gtaaataatt
    52441 acatcaccgt aaaaaatatc attacggctg ccggtatcga acaaataaaa cattttctgg
    52501 cgacggcatt tattcgccag caaccggagc gttttttctc tgcccccgga agttttcatt
    52561 attcgaatgt acgcggtgaa agctggcaac atattacccg gcaaatttgt gctcaattag
    52621 tcgcacaaca ccatattacc gccgatgaag cgcaacggat catcgcccgc gaaggcgaag
    52681 gtgaaaacct gattgttaat cgcctcgcca tcccacattg ctggagcgaa caggagcgac
    52741 gttttcgtgg attttttatt accctcgccc aaccggttga ggtgaataac gaagtagtta
    52801 accatgtact gatcgcctgt gccgccgccg atgcgcgtca tgagctaaaa atatttagct
    52861 atctggcaag cgtattgtgc cagcatccgg cagaggttat tgccgggtta acaggatatg
    52921 aggcatttat ggagttactt cacaaggggt gaactgtgtc ggatgcgccg catccgacac
    52981 tgcatttatc accaaaatca aaaacgttta acgcaagcta ttatagtcat cgaccatttc
    53041 gttgtactcg cgtaacgctc gtggaaatga gtcgtcgacc atccagcttg tgtttgcatc
    53101 ctgaagttgc tctttatccc agtcagaata cggaacttta tctctgacgc ggcgaacata
    53161 ttttttagcc tcgagttgat actggtctgc cgaattaatg aacgagaaat tcataccgcc
    53221 tttatccgct tctttggcct gcgccaccag cgtttccagt tcagacactt tcttcattgc
    53281 cgcatcaaca tcaaacttct cttgcgatat caggttatta atttgtttcg ctgaaatcat
    53341 gactgccaga gaatagtagt ggaatgtttt cccttctctt tcttcaatat ttttcagttc
    53401 cgcaagctga cgcttatcat taatttcctg gatcgccgca tgataagatt cggctacagg
    53461 ttcaaaggct tccagatttt ttaagaatgt ctggtgcagc gttttccctt tcgcaaacgc
    53521 atcgtcttta taattttcct gggtgtaata tttatccatt tcgttaatgg tattacccaa
    53581 cgccacggca gcatcgatat aactcaccgc tacgccatca ataggttgca gcgcaggcgt
    53641 taacgccaca gcacttttta cgcctttttc acactcagcg aggctggatt cactaaagcc
    53701 gtagatgcca taaacagtac tctctttacc ggtaggcccc tgtttaaaat ctttcagcca
    53761 gtcagcataa cgcgccagac tgcgctgtac cgggatctgt aacttgttgt agcacttgat
    53821 atagacatcc attttttcgt cgaaaaccgc ttgttcatcg accaccggtt gggcaggagt
    53881 ttcaggttta acttctgctt ttgcaacggg agcttcagca ggtttcgctt caggagctgg
    53941 tgcggcaggt tgactattgg caggcggggg cgtttctgtt tcggcttttt tatcatcaca
    54001 acccgtgaga ccgagggcca tgatggcgac tataatagcg gaagataata aattcctttt
    54061 catttaattg ccttataaat atttagataa gagagataat attttaaatg aaaaaatgat
    54121 ccgtcactat acacattttg cttataaaca aaccgaaatc attcacactc cgaataattc
    54181 ctgcgactta aaatataaat cgcaggaagc gattttaatt acccgctcta ccactggcgc
    54241 aggatataaa ctctcaccta cattccataa cgttcagaaa tagcagcatg cttctcttca
    54301 ttcgccccgg cttctttggc aagacgccac cagcacaacg gatgttccca gtcagcggtc
    54361 aattcgtgca aggtcttatc gatatcacct ttagaaactg aacgcggcgc gctgataaca
    54421 aagtagaacg cgggcagcgg ctgtgcatct tctccctgct ggctgtccgt taacgtatag
    54481 tcctcgttac tgttgctcca gtagtaccag tttcgttcac ggagcgcctc acggaaggca
    54541 ttaaactgtt cttcaccagg catgacacct tcgcagaacc aggactcgcc gtactcagtc
    54601 gggataatgg tttgcaggca ggcataaagt ggcgtgaaat tcttccgttc ttcctcatcc
    54661 tctggttggg tgttcaatat ttccgcatcg aataccacaa cttcatcaac atgttgcgga
    54721 taatcggtcc ggtaagttgg ctcaatgatc cttgctgtta ccggcccaat acgttgagca
    54781 tagtaatagc ggtattcata attttcccgg aagcgaatgt ttagcagtcc ccatttttcg
    54841 tcgataaaac cttcaccttc cgtaagttcg atttccagtt cagctgcaat agcacgaacc
    54901 aaatcccatt gttgcaggat gctggcggtc gccatgatat cccactggat attacgaata
    54961 tcttcttcac ggctaatcat cagttgctgc aattgttgta aataatccag ctgtcgatcc
    55021 caggcttcct gtttttgtgc tgcccaggag cacatgcgat aaacgccgat ccactcacgc
    55081 cctttttcgt tttgcagcaa ctggtgccca atcgactcga gttcactcca gtcttcctgt
    55141 tggttgatca ctcgcgcacg gaaccacaag gccatatgta gcagccccgc ctgttcatac
    55201 tgcttcgcca ggtgctcaat ggtttgcaga tcgtcacggt ggagcaataa atccaacaga
    55261 tcgaagcaaa gaccattatc ttcctggatg tggctttcga cgtagcgttg cagaagttca
    55321 atcgactcat caatcgcatt acaggcgttt aacgcattga ccagtttgcc cagcaattcc
    55381 gcatcatctg gcgcgttcag gtaagcgcgg cggaatgttt cgacgtcttt ttctggatct
    55441 ggctgcgttt cttcatcgct attttcaggt acttctacaa ccggacgagc aagcaactct
    55501 gcttctttgg cggcgatttt ttcactcagt tcgagaaact cacgatcaac ccccagtaac
    55561 acgcgtaatt ttggcatgtg tcgctgggcc agtttcatcg ctattttggc aacccagacg
    55621 gattcacgct ccagcgccag ggtaatttgc agtttcgcca gatcaaccac tttgcggtgc
    55681 gctccggcct gcgagcggtg ctccagcatt ttttggatga tgcgcgccag ttgccagttg
    55741 tttctttgtg gatgacgggg aagtaacaca gtaatgattt tcagccatga cccccatgaa
    55801 tggggagtca gatctgacca acgcggtaat tcctcccaga cttcttcgtc ctgcccgcat
    55861 aacgccatga tgtacagttt tttgaggtga ttactaatgc ggtcgcggcc ccagtaatca
    55921 ttggcttctg tcagctcatc aatcagtatg cgtgcttcat cataacgctg aagttcaatc
    55981 agaatatcgg cacgagtata aagtaatgca tcaatatcat cttccccggc tgctttaact
    56041 gcgtcgattt gtcgatccag ataagccagt gcctcctcta ttcttttgga atctatcagt
    56101 gcactggcat attcagtgga caaacactga aaacagctcc aggtcggatc aatccgcgcg
    56161 atggtttctt cacataccgc catacgttca ttcgcccagc caggtgcatc aatattttcg
    56221 tagcaggcgg ataaatcctg ggtaacgcag atagattgtg ggcaatcttt tgtctcttcc
    56281 cggtgcgctt tttcaaacag cgctaccacg tctggcagca ccttttcacc ttcttcatag
    56341 ttagtcaggc gatgacgcat ttcccagtga ccaataaata tttccagcca cggatttttt
    56401 gccacctggg taagtgcttt tacttcaggt aacagcgaat cgcacttttc catttgcagg
    56461 tgcataacgc cttccgaata atcatcgata agttgcggaa tatactcctg cccggcatcg
    56521 cgtaactcct tagtatattt gtgataccac tcccatatat ccattttatt ttccttgatt
    56581 aattaagaca ggaataacct gagtaaaatc ttccagtgcc tggtgtaaat cccactgctg
    56641 ttctttatta ccgctggagc acaaaatcac tttcagcgat tttaataagc gtaatgccgc
    56701 tgcgggctgt tgctggtgtt gtaatgccgt caccagttgc atgatgcaag ggttattaag
    56761 gttgatgtat aaacttgagg ttttcgtttt ttggatttgt gaagtaaatt gccgcgccag
    56821 cattaacgct gcggtgctga tgcgtttatc tgcgtcatcc tgttcgagaa tttgttttaa
    56881 ttctgcttcg cggtctggcg taaccactaa cggtaaaacg gcgggttcga agcgggagat
    56941 aattaattgc tcgccatcgc agagatgctc ttccagataa gctacctgct cttcgggtaa
    57001 ttccgccagg ctgaacaact gctcattacc tgtttgcgta ccgacttcga caatccggca
    57061 gtgatataac agcgcccagc gacgtaaaaa tggcacgacg gcatagcgat cgccacgggc
    57121 aacgggccgt tgcagaatgt ggaacaacat ctcttcaaaa ccgccgtcgc ggcttaacag
    57181 aatatgaatg ctgttattaa cgcgtaaatc cttcgccagc aacgcccctt ttgacgttgg
    57241 cacctgcaag cgatctttga gcaaatcaaa cagacggtca tcacataatg ccgcaccgag
    57301 caacgcttcg ttgtggcgca ttaatacacg ccgccagatt tctggctgat tttgtgcgag
    57361 atcggacaaa ccagaaatca gcgcctcttt taacgcctcc tgcaccgcaa cccaggtttc
    57421 gtcccgctgg agatcttccc ggctcgccgt aggcgttagt ttcgatgact caatcacgcc
    57481 gccaataaat ccggcccagg gaggtaacaa ctcacgcgct tcatcatcca gtaacatacc
    57541 gcgcaaaaac agcgacaggt tgcggttatc gctggtgccg taggttgcgc catcctgaat
    57601 ccataaaatc ccaaccgcgt cgctcatccc cacgggcacc accggaatgg tgcaaatcgg
    57661 ttcgaaggag gattcaaact gggcggcaaa ggcgagattt ttacgctgta ccagcgcgcg
    57721 gtgcatggtt acgccttcgg gggcaacttc acgccacggt ggttgaagtt tatttaccgg
    57781 ctcgctggca tcgccgacat agaccggttc gtgcagcaat atacagtagc gggaaagaac
    57841 gcggttcagc aaattgttac tcgccagatg gctgtactct tctttgagcg tcagaatcac
    57901 ctgcgtaccc gtttccgagg actgatgcgg cgtaacggta tatttttgcc cgtcggtaga
    57961 gtggtatttc cagctctgat ccggcgtttg ccaggatgtg gtcaacaccg tgacctcttt
    58021 cgccaacaca aaggccgaca aaaaaccgag gccgaacata ccaattaaac cggtgttgtc
    58081 atcctgctgg cgcaacattc gggtataacc cacgcctact gtcgccagga agccgtgaat
    58141 ttcactttct gtcagtccag cgccagtatc gctaatagtg atagtggact tcgccacgtc
    58201 tgccaccaca cgaatcgcgt tatcctttgg tgcatcgggc tgctcaatcc tgcggcgaac
    58261 gatcgcatca tggccgttct gcaccagctc gcgcacggca accacgggag tggagtaaag
    58321 atgcttactg agcacctcaa ttaagccgtt taaattaact tctgtagaat atgaactacc
    58381 aggtaactgt aatgtactca tttttcaata ctcataatcc atcacctact attgaaaata
    58441 catcgctgtt tttacaaccg gtgcaaggtt tgttatttca ttcactcatc ggaaacattt
    58501 atttattgac gcgatgatta aaacaaaatg tcttatataa ataatgatta attcttaaag
    58561 gattaatgcg actggagtat atcgatataa cccggagtta acgagataat ttcttgtggt
    58621 gaaattatta tttgaatgct gaataatata ttgaggatta tttaatagaa aaataaaacc
    58681 gcaatccaga atcagaattg cggtatttct ttaattatga atcaaacgct gcggatgggt
    58741 ataaaccgtt gcccgacccg gtttacaaaa acctaccagt gtcagattac agcgctcagc
    58801 cacttctaca gcaagcgtgg tcgcggcaga caccgcaaac aaaatctcta cgccgcacat
    58861 cgctgacttt tgcaccattt cataactggc acggctggaa accagcaccg caccttgctg
    58921 ccagctttcc ccttcttgtg accggcggcc taacagtttg tccagcgcca catggcgacc
    58981 cacgtcttca tgcccgccga ccagttcgcc agatggcaac atccaggcgg cggcgtgagt
    59041 acaaccggtc agttgcccca ctggctgaaa atcattgaga tgacgtaatg catcatccag
    59101 tttgttgaga tcaaacgtct gggtgaacgg tagcggctgc accggttttc cgatgtcatt
    59161 aagttgctcc acgccgcaca cgccacatcc cgtacgtccc gccagtgccc ggcgacgctc
    59221 cttcaacccc ataaagcggc ggctggaaag ctcaatttgc acttccagac cattacagga
    59281 aggaacgaca tccataccga agatatcgcg cggactttcg ataatcccct cggaaagcga
    59341 aaaaccgagc gcaaagtact caaggtcttt gggtgacgcc atcatcacta catgcgaaat
    59401 gccgttgtag accagcgcaa cgggaacctc ttccgccacc tcatctaacc gtgggtgttg
    59461 taaatcatca cgccgccata actcaatttg acgaacacct gtgacacttg tcacattttc
    59521 gatttctttt tgctgtgttt ttttcacttt ctcttaacca gataagaaca cacacaccaa
    59581 cattatggta ttctgttaca aacccttcct ggatggaggg aaattgagcc aattctggac
    59641 ctttgcggcc ccttccgcaa agaaaaataa ctcccactcc ctgcacacgc agcaagcgaa
    59701 tgtaaatggg acgtgacaat gtcgaaacaa ggagcaatcc atgcaggtca gcagaaggca
    59761 gttctttaag atctgcgctg gcggtatggc aggcaccacg gcagcggcac tgggttttgc
    59821 acccagcgta gcactcgcgg aaacccggca gtataaactg ctgcgcaccc gcgaaacccg
    59881 taatacctgc acctattgtt ccgtaggctg tgggctgttg atgtacagcc tcggtgacgg
    59941 agcaaaaaac gccaaagcat ctatcttcca tatcgaaggt gacccggatc acccggtcaa
    60001 ccgcggagca ctttgtccga aaggcgctgg cctggtggat ttcatccact ccgaaagccg
    60061 cctgaagttt ccggaatacc gtgcgccagg ttctgataaa tggcaacaaa tcagttggga
    60121 agaggcgttt gatcgcatcg ccaaactgat gaaagaagac cgcgatgcga actacattgc
    60181 gcaaaacgcc gaaggcgtga ctgttaaccg ctggctctcc accgggatgc tgtgtgcttc
    60241 cgcgtcgagc aacgaaaccg gctatttaac acaaaaattc tcccgcgcgc tgggtatgct
    60301 cgcggtcgac aaccaggcgc g
//
LOCUS       DAMZFT010000013        58282 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.013, whole genome shotgun sequence.
ACCESSION   DAMZFT010000013 DAMZFT010000000
VERSION     DAMZFT010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 58282)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 58282)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..58282
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.013"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(<1..121)
                     /locus_tag="PN977_000942"
     CDS             complement(<1..121)
                     /locus_tag="PN977_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418394.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HDI6041005.1"
                     /translation="MMNPLIIKLGGVLLDSEEALERLFSALVNYRVSHQRPLVI"
     gene            complement(129..1133)
                     /gene="argC"
                     /locus_tag="PN977_000943"
     CDS             complement(129..1133)
                     /gene="argC"
                     /locus_tag="PN977_000943"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HDI6041006.1"
                     /translation="MLNTLIVGASGYAGAELVTYVNRHPHMNITALTVSAQSNDAGKL
                     ISDLHPQLKGIVDLPLQPMSDISEFSPGVDVVFLATAHEVSHDLAPQFLEAGCVVFDL
                     SGAFRVNDAAFYEKYYGFTHQYPELLEQAAYGLAEWCGNKLKEANLIAVPGCYPTAAQ
                     LALKPLIDADLLDLIQWPVINATSGVSGAGRKAAISNSFCEVSLQPYGVFTHRHQPEI
                     ATHLGADVIFTPHLGNFPRGILETITCRLKSGVTQAQVAQVLQQAYAHKPLVRLYDKG
                     VPALKNVVGLPFCDIGFAVQGEHLIIVTTEDNLLKGAAAQAVQCANIRFGYAETQSLI
                     "
     gene            1287..2438
                     /gene="argE"
                     /locus_tag="PN977_000944"
     CDS             1287..2438
                     /gene="argE"
                     /locus_tag="PN977_000944"
                     /EC_number="3.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine deacetylase"
                     /protein_id="HDI6041007.1"
                     /translation="MKNKLPPFIEIYRALIATPSISATEEALDQSNADLITLLADWFK
                     DLGFNVEVQPVPGTRNKFNMLASTGQGAGGLLLAGHTDTVPFDDGRWTRDPFTLTEHD
                     GKLYGLGTADMKGFFAFILDALRDVDVTKLAKPLYILATADEETSMAGARYFAETTAL
                     RPDCAIIGEPTSLQPVRAHKGHISNAIRIQGQSGHSSDPARGVNAIELMHDAIGHILQ
                     LRDKLKERYHYEAFTVPYPTLNLGHIHGGDASNRICACCELHMDIRPLPGMTLNELNG
                     LLNDALAPVSERWPGRLTVDELHPPIPGYECPPNHQLVEVVEKLLGAKTEVVNYCTEA
                     PFIQTLCPTLVLGPGSINQAHQPDEYLETRFIKPTRELITQVIHHFCWH"
     gene            2740..5391
                     /gene="ppc"
                     /locus_tag="PN977_000945"
     CDS             2740..5391
                     /gene="ppc"
                     /locus_tag="PN977_000945"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004203687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HDI6041008.1"
                     /translation="MNEQYSALRSNVSMLGKVLGETIKDALGEHILERVETIRKLSKS
                     SRAGNDANRQELLTTLQNLSNDELLPVARAFSQFLNLANTAEQYHSISPKGEAASNPE
                     VIARTLRKLKNQPELSEDTIKKAVESLSLELVLTAHPTEITRRTLIHKMVEVNACLKQ
                     LDNKDIADYEHNQLMRRLRQLIAQSWHTDEIRKLRPSPVDEAKWGFAVVENSLWQGVP
                     NYLRELNEQLEENLGYKLPVEFVPVRFTSWMGGDRDGNPNVTADITRHVLLLSRWKAT
                     DLFLKDIQVLVSELSMVEATPELLALVGEEGAAEPYRYLMKNLRSRLMATQAWLEARL
                     KGEELPKPEGLLTQNEELWEPLYACYQSLQACGMGIIANGDLLDTLRRVKCFGVPLVR
                     IDIRQESTRHTEALGELTRYLGIGDYESWSEADKQAFLIRELNSKRPLLPRNWQPSAE
                     TREVLDTCQVIAEAPQGSIAAYVISMAKTPSDVLAVHLLLKEAGIGFAMPVAPLFETL
                     DDLNNANDVMTQLLNIDWYRGLIQGKQMVMIGYSDSAKDAGVMAASWAQYQAQDALIK
                     TCEKAGIELTLFHGRGGSIGRGGAPAHAALLSQPPGSLKGGLRVTEQGEMIRFKYGLP
                     EITVSSLSLYTGAILEANLLPPPEPKESWRRIMDELSVISCDLYRGYVRENKDFVPYF
                     RSATPEQELGKLPLGSRPAKRRPTGGVESLRAIPWIFAWTQNRLMLPAWLGAGTALQK
                     VVEDGKQSELEAMCRDWPFFSTRLGMLEMVFAKADLWLAEYYDQRLVDKALWPLGKEL
                     RNLQEEDIKVVLAIANDSHLMADLPWIAESIQLRNIYTDPLNVLQAELLHRSRQAEKE
                     GHEPDPRVEQALMVTIAGIAAGMRNTG"
     gene            5574..7307
                     /locus_tag="PN977_000946"
     CDS             5574..7307
                     /locus_tag="PN977_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase CptA"
                     /protein_id="HDI6041009.1"
                     /translation="MHSTEVQAKPLFSWKALGWALLYFWFFSTLLQAIIYISGYSGTN
                     GIRDSLLFSSLWLIPVFLFPKRIKIIAAVIGVVLWAASLAALCYYVIYGQEFSQSVLF
                     VMFETNTNEASEYLSQYFSLKIVLIALAYTAVAVLLWTRLRPVYIPKPWRYVVSFALL
                     YGLILHPIAMNTIIKGKPIEKTLDSLASRMEPAAPWQFISGYYQYRQQLNSLTKLLNE
                     NNALPPLANFKDESGNEPRTLVLVIGESTQRGRMSLYGYPRETTPELDALHKTDPNLT
                     VFNNVVTSRPYTIEILQQALTFANEKNPDLYLTQPSLMNMMKQAGYKTFWITNQQTMT
                     ARNTMLTVFSRQTDKQYYMNQQRTQSAREYDTNVLKPFQEVLKDPAPKKLIIVHLLGT
                     HIKYKYRYPENQGKFDGNTDHVPPGLNAEELESYNDYDNANLYNDHVVASLIKDFKAA
                     DPNGFLVYFSDHGEEVYDTPPHKTQGRNEDNPTRHMYTIPFLLWTSEKWQATHPRDFS
                     QDVDRKYSLAELIHTWSDLAGLSYDGYDPTRSVVNPQFKETTRWIGNPYKKNALIDYD
                     TLPYGDQVGNQ"
     gene            7456..8307
                     /locus_tag="PN977_000947"
     CDS             7456..8307
                     /locus_tag="PN977_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDI6041010.1"
                     /translation="MYHDVSYLLSRLINGPLSLRQIYFASSNGPVPDLAYQVDFPRLE
                     IVLEGEFVDTGAGATLVPGDVLYVPAGGWNFPQWQAPATTFSVLFGKQQLGFSVVQWD
                     GKQYQNLAKQHVARRGPRIGSFLLQTLNEMQMQPQEQQTARLIVASLLSHCRDLLGSQ
                     IQTASRSQALFEAIRDYIDERYASALTRESVAQAFYISPNYLSHLFQKTGAIGFNEYL
                     NHTRLEHAKTLLKGYDLKVKEVAHACGFVDSNYFCRLFRKNTERSPSEYRRQYHSQLT
                     EKPTTPE"
     gene            complement(8294..8635)
                     /locus_tag="PN977_000948"
     CDS             complement(8294..8635)
                     /locus_tag="PN977_000948"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HDI6041011.1"
                     /translation="MAYLVAVTACVSGVAHTYMAAERLEKLCLLEKWGVSIETQGALG
                     TENRLADEDIRRADVALLITDIELAGAERFEHCRYVQCSIYAFLREPQRVMSAVRKVL
                     SAPQQTHLILE"
     gene            complement(8637..9515)
                     /locus_tag="PN977_000949"
     CDS             complement(8637..9515)
                     /locus_tag="PN977_000949"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418387.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[formate-C-acetyltransferase]-activating enzyme"
                     /protein_id="HDI6041012.1"
                     /translation="MTSSAGQRISCNVVETRRNDVARIFNIQRYSLNDGEGIRTVVFF
                     KGCPHLCPWCANPESISGKIQTVRREAKCLHCAKCLRDADECPSGAFERIGRDISLDA
                     LEREVMKDDIFFRTSGGGVTLSGGEVLMQAEFATLFLQRLRLWGVSCAIETAGDAPAS
                     KLLPLAKLCDEVLFDLKIMDATQARDVVKMNLPRVLENLRLLVSEGVNVIPRLPLIPG
                     FTLSRENMQQALDVLIPLNIRQIHLLPFHQYGEPKYRLLGKTWSMKEVSAPSSADVAT
                     MREMAERAGFQVTVGG"
     gene            complement(9481..11778)
                     /locus_tag="PN977_000950"
     CDS             complement(9481..11778)
                     /locus_tag="PN977_000950"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HDI6041013.1"
                     /translation="MTNRISRLKTALFANTREISLERALLYTASHRQTEGEPVILRRA
                     KATAYILEHVEISIRDEELIAGNRTVKPRAGIMSPEMDPYWLLKELDQFPTRPQDRFA
                     ISEEDKRIYREELFPYWEKRSMKDFINGQMTDEVKAATSTQIFSINQTDKGQGHIIID
                     YPRLLNHGLGELVAQMQQHCQQQPENHFYQAALLLLEASQKHILRYAELAETMAASCT
                     DGQRREELLTIAEISRHNAEHKPQTFWQACQLFWYMNIILQYESNASSLSLGRFDQYM
                     LPFYQASLTQGEDPAFLKELLESLWVKCNDIVLLRSTSSARYFAGFPTGYTALLGGLT
                     ENGRSAVNVLSFLCLDAYQSVQLPQPNLGVRTNALIDTPFLMKTAETIRLGTGIPQIF
                     NDEVVVPAFLNRGVSLEDARDYSVVGCVELSIPGRTYGLHDIAMFNLLKVMEICLHEN
                     EGNAALTYEGLLEQIRAKISHYITLMVEGSNICDIGHRDWAPVPLLSSFISDCLEKGR
                     DITDGGARYNFSGVQGIGIANLSDSLHALKGMVFEQQRLSFDELLSVLKANFATPEGE
                     KVRARLINRFEKYGNDIDEVDNISAELLRHYCKEVEKYQNPRGGYFTPGSYTVSAHVP
                     LGSVVGATPDGRFAGEQLADGGLSPMLGQDAQGPTAVLKSVSKLDNTLLSNGTLLNVK
                     FTPATLEGEAGLRKLADFLRAFTQLKLQHIQFNVVNADTLREAQQRPQDYAGLVVRVA
                     GYSAFFVELSKEIQDDIIRRTAHQL"
     gene            complement(11829..12149)
                     /locus_tag="PN977_000951"
     CDS             complement(11829..12149)
                     /locus_tag="PN977_000951"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HDI6041014.1"
                     /translation="MTKIIAVTACPSGVAHTYMAAEALESAAKAKGWEVKVETQGSIG
                     LENELTAEDVASADMVILTKDIGIKFEERFAGKTIVRVNISDAVKRADAIMSKIEAHL
                     AQTA"
     gene            complement(12164..13243)
                     /locus_tag="PN977_000952"
     CDS             complement(12164..13243)
                     /locus_tag="PN977_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit EIIC"
                     /protein_id="HDI6041015.1"
                     /translation="MNELVQILKNTRQHLMTGVSHMIPFVVSGGILLAVSVMLYGKGA
                     VPDAVADPNLKKLFDIGVAGLTLMVPFLAAYIGYSIAERSALAPCAIGAWVGNSFGAG
                     FFGALIAGIIGGIVVHYLKKIPVHKVLRSVMPIFIIPIVGTLITAGVMMWGLGEPVGA
                     LTNSLTQWLQGMQQGSIVMLAVIMGLMLAFDMGGPVNKVAYAFMLICVAQGVYTVVAI
                     AAVGICVPPLGMGLATLIGRKNFSAEERETGKAALVMGCVGVTEGAIPFAAADPLRVI
                     PSIMIGSVCGAVTAALVGAQCYAGWGGLIVLPVVEGKLGYIAAVAVGAVVTAVCVNVL
                     KSLARKNGSSTDEKEDDLDLDFEIN"
     gene            13552..16053
                     /gene="ptsP"
                     /locus_tag="PN977_000953"
     CDS             13552..16053
                     /gene="ptsP"
                     /locus_tag="PN977_000953"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HDI6041016.1"
                     /translation="MALIVEFICELPNGVHARPASHVETLCNTFSSQIEWHNLRTDRK
                     GNAKSALALIGTDTLVGDNCQLLISGADEQEAHQRLSQWLRDEFPHCDAPLAEVKSDE
                     LEPLPVSLTNLNPQIIRARTVCSGSAGGILTPISSLDLNALGNLPAAKGVDAEQSALE
                     NGLTLVLKNIEFRLLDSDGATSAILEAHRSLAGDTSLREHLLAGVSAGLSCAEAIVAS
                     ANHFCEEFARSSSSYLQERALDVRDVCFQLLQQIYGEQRFPAPGKLTQPAICMADELT
                     PSQFLELDKNHLKGLLLKSGGTTSHTVILARSFNIPTLVGVDIDALTPWQHQTIYIDG
                     NAGAIVVEPGESVARYYQQEARVQDALREQQRVWLTQQARTADGIRIEIAANIAHSVE
                     AQAAFGNGAEGVGLFRTEMLYMDRTSAPGESELYNIFCQALESANGRSIIVRTMDIGG
                     DKPVDYLNIPAEANPFLGYRAVRIYEEYASLFTTQLRSILRASAHGSLKIMIPMISSM
                     EEILWVKEKLAEAKQQLRNEHIPFDEKIQLGIMLEVPSVMFIIDQCCEEIDFFSIGSN
                     DLTQYLLAVDRDNAKVTRHYNSLNPAFLRALDYAVQAVHRQGKWIGLCGELGAKGSVL
                     PLLVGLGLDELSMSAPSIPAAKARMAQLDSRECRQLLNQAMACRTSLEVEHLLAQFRM
                     TQQDAPLVTAECITLESDWRSKEEVLKGMTDNLLLAGRCRYPRKLEADLWAREAVFST
                     GLGFSFAIPHSKSEHIEQSTISVARLAAPVRWGDDEAQFIIMLTLNKHAAGDQHMRIF
                     SRLARRIMHEEFRNALVNAASADAIASLLQHELEL"
     gene            16065..16727
                     /gene="fsa"
                     /locus_tag="PN977_000954"
     CDS             16065..16727
                     /gene="fsa"
                     /locus_tag="PN977_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HDI6041017.1"
                     /translation="MELYLDTANVAEVERLARIFPIAGVTTNPSIIAASKESIWEVLP
                     RLQKAIGDEGILFAQTMSRDAQGMVEEAKRLRDAIPGIVVKIPVTSEGLAAIKMLKKE
                     GITTLGTAVYSAAQGLLAALAGAKYVAPYVNRVDAQGGDGIRTVQELQALLEMHAPES
                     MVLAASFKTPRQALDCLLAGCESITLPLDVAQQMLNTPAVESAIEKFEHDWNAAFGTT
                     HL"
     gene            16738..17841
                     /gene="gldA"
                     /locus_tag="PN977_000955"
     CDS             16738..17841
                     /gene="gldA"
                     /locus_tag="PN977_000955"
                     /EC_number="1.1.1.6"
                     /EC_number="1.1.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418380.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional L-1,2-propanediol
                     dehydrogenase/glycerol dehydrogenase"
                     /protein_id="HDI6041018.1"
                     /translation="MDRIIQSPGKYIQGADVINRLGEYLKPLAERWLVVGDKFVLGFA
                     QSTVEKSFKDAGLVVEIAPFGGECSQNEIDRLRGIAETAQCGAILGIGGGKTLDTAKA
                     LAHFMGVPVAIAPTIASTDAPCSALSVIYTDEGEFDRYLMLPNNPNMVIVDTKIVAGA
                     PARLLAAGIGDALATWFEARACSRSGATTMAGGKCTQAALALAELCYNTLLEEGEKAM
                     LAAEQHVVTPALERVIEANTYLSGVGFESGGLAAAHAVHNGLTAIPDAHHYYHGEKVA
                     FGTLTQLVLENAPVEEIETVAALSHAVGLPITLAQLDIKEDVPAKMRIVAEAACAEGE
                     TIHNMPGGATPDQVYAALLVADQYGQRFLQEWE"
     gene            18116..18733
                     /locus_tag="PN977_000956"
     CDS             18116..18733
                     /locus_tag="PN977_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1287 domain-containing protein"
                     /protein_id="HDI6041019.1"
                     /translation="MKASLVLVSLLTAITSHSLKSPAVPPTVVQIQANTNLAIADGAR
                     QQIGSTLFYDPAYVQLTYPGGDVPQERGVCSDVVIRALRSQKVDLQKLVHEDMAKNFA
                     AYPQKWQLKRPDSNIDHRRVPNLETWFTRHDKTRPTSKNPSDYQAGDIVSWRLDNGLA
                     HIGVVSDGFARDGTPLVIHNIGAGTQEEDVLFSWRMVGHYRYFAK"
     gene            complement(18758..19663)
                     /gene="yijE"
                     /locus_tag="PN977_000957"
     CDS             complement(18758..19663)
                     /gene="yijE"
                     /locus_tag="PN977_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418378.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine transporter YijE"
                     /protein_id="HDI6041020.1"
                     /translation="MSAAGKSNPLAISGLVVLTLIWSYSWIFMKQVTSYIGAFDFTAL
                     RCIFGALVLFIVLLLRGRGMRPTPFKYTLAIALLQTCGMVGLAQWALVSGGAGKVAIL
                     SYTMPFWVVIFAALFLGERLRRGQYFAILIAAFGLFLVLQPWQLDFSSMKSAMLAILS
                     GVSWGASAIVAKRLYARHPRVDLLSLTSWQMLYAALVMSVVALLVPQREIDWQPTVFW
                     ALAYSAILATALAWSLWLFVLKNLPASIASLSTLAVPVCGVLFSWWLLGENPGAVEGS
                     GIVLIVLALALVSRKKKEAVSVKRI"
     gene            complement(19757..21937)
                     /gene="katG"
                     /locus_tag="PN977_000958"
     CDS             complement(19757..21937)
                     /gene="katG"
                     /locus_tag="PN977_000958"
                     /EC_number="1.11.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase/peroxidase HPI"
                     /protein_id="HDI6041021.1"
                     /translation="MSTSDDIHNTTATGKCPFHQGGHDQSAGGGTTTRDWWPNQLRVD
                     LLNQHSNRSNPLGEDFDYRKEFSKLDYYGLKKDLKALLTESQPWWPADWGSYAGLFIR
                     MAWHGAGTYRSIDGRGGAGRGQQRFAPLNSWPDNVSLDKARRLLWPIKQKYGQKISWA
                     DLFILAGNVALENSGFRTFGFGAGREDVWEPDLDVNWGDEKAWLTHRHPEALAKAPLG
                     ATEMGLIYVNPEGPDHSGEPLSAAAAIRATFGNMGMNDEETVALIAGGHTLGKTHGAG
                     PTSNVGPDPEAAPIEEQGLGWASTYGSGVGADAITSGLEVVWTQTPTQWSNYFFENLF
                     KYEWVQTRSPAGAIQFEAVDAPEIIPDPFDPSKKRKPTMLVTDLTLRFDPEFEKISRR
                     FLNDPQAFNEAFARAWFKLTHRDMGPKSRYIGPEVPKEDLIWQDPLPQPIYNPTEQDI
                     IDLKFAIADSGLSVSELVSVAWASASTFRGGDKRGGANGARLALMPQRDWDVNAAAVR
                     ALPVLEKIQKESGKASLADIIVLAGVVGVEKAASAAGLSIHVPFAPGRVDARQDQTDI
                     EMFELLEPIADGFRNYRARLDVSTTESLLIDKAQQLTLTAPEMTALVGGMRVLGANFD
                     GSKNGVFTDRVGVLSNDFFVNLLDMRYEWKATDESKELFEGRDRETGEVKYTASRADL
                     VFGSNSVLRAVAEVYASSDAHEKFVKDFVAAWVKVMNLDRFDLL"
     gene            complement(22266..23156)
                     /gene="metF"
                     /locus_tag="PN977_000959"
     CDS             complement(22266..23156)
                     /gene="metF"
                     /locus_tag="PN977_000959"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HDI6041022.1"
                     /translation="MSFFHASQRDALNQSLAEVQGQINVSFEFFPPRTSEMEQTLWNS
                     IDRLSNLKPKFVSVTYGANSGERDRTHSIIKGIKDRTGLEAAPHLTCIDATPDELRTI
                     ARDYWNNGIRHIVALRGDLPPGSGKPEMYASDLVTLLKEVADFDISVAAYPEVHPEAK
                     SAQADLLNLKRKVDAGANRAITQFFFDVESYLRFRDRCVSAGIDVEIIPGILPVSNFK
                     QAKKFADMTNVRIPAWMAQMFDGLDDDAETRKLVGANIAMDMVKILSREGVKDFHFYT
                     LNRAEMSYAICHTLGVRPGL"
     gene            complement(23538..25091)
                     /locus_tag="PN977_000960"
     CDS             complement(23538..25091)
                     /locus_tag="PN977_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HDI6041023.1"
                     /translation="MKIKMLAAGIALTLPFWACAKDVTIIYTNDLHAHVEPYKVPWIA
                     DGKRDIGGWANITTLVKQEKAKNKATWFFDAGDYFTGPYISSLTKGKAIIDIMNTMPF
                     DAVTIGNHEFDHGWDNTLLQLSQAKFPIVQGNVFYQNSSKSFWDKPYTIIEKDGVKIG
                     VIGLHGVFAFNDTVSAATRVGIEARDEIKWLQRYIDELKGKVDLTVALIHEGVPARQS
                     SMGGTDVRRALDKDIQTASQVKGLDILITGHAHVGTPEPIKVGNTLILSTDSGGIDVG
                     KLVLDYKEKPHTFTVKNFELKTLYADEWKPDPQTKQVIDSWNKKLDEVVQQTVAQSPV
                     ELKRAYGESASLGNLAADALLAAAGKNTQLALTNSGGIRNEIPAGAITMGGVISTFPF
                     PNELVTMDLTGKQLRSLMEHGASLSNGVLQVSKGLEMKYDSSKPVGQRVITLTLNGKP
                     IEDATIYHIATQSFLADGGDGFTAFTEGKARNTTGGYYVYHAVVDYFKAGNTITDEQI
                     NGMRVKDIK"
     gene            25224..26351
                     /locus_tag="PN977_000961"
     CDS             25224..26351
                     /locus_tag="PN977_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001765453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytoplasmic protein"
                     /protein_id="HDI6041024.1"
                     /translation="MSNLFAINVCKTFGCRNLGLASSEDYSWPDYKLGFPALHCQACG
                     SYPPLFDEQQFRDWLSVHMTACAIETGHFCPSCYCKEKIFYGHNPQGTQRVQCRSCKK
                     VWTPKQQPAREITYPQSIETVQLFMPFQGASAVQKLYVLVSLDATRGNILHLSTNYTQ
                     HQTGDSLRYSYRGNTEPTEHHSDIVQRVDMREAQFLRRSQFDEIQYGSAVLKRNGKGA
                     ILRPVITAHGHFRILKIRFPHVKTHIISHECFLRGAIITAWADQFRQQQGELWFVEEE
                     ISDRNADIPWHFQGTTYHGWWQNQWQRWGQGNNSKMVCLLTGVSLERGANVSLATSRC
                     FITWLTDQHDFTQSALLSAGRVTKMLTSLALKYNESLTPSC"
     gene            26584..28134
                     /locus_tag="PN977_000962"
     CDS             26584..28134
                     /locus_tag="PN977_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HDI6041025.1"
                     /translation="MRTVFTTLSVIFSVMFSHSILAQDVTIYYTNDIHAHVNPAKIPA
                     VDKNRLVGGMANIAGIVNEAKKKNKDVFFFDAGDYFTGPYISTLTKGEAIIDIMNTMP
                     FDAVSVGNHEFDHGVPNMVSQLSKAKFPILLGNIYYTDTNKPVWDHPWTIIEKDGLKI
                     GVIGLHGAFAFYDTVAAKAREGVEARDEIKYLNKALAELKGKVDITVLLIHEGVPARQ
                     SSFGSKDVERLLQADIETAKKVNGVDVLITGHAHVGTPQPIKVNNTLIVSTDAYGTDI
                     GKLVLDYNPKTKKIDSYNGELITIFADQFKPDTIVQNTIDKWSVKLNKITQEVVGHSP
                     VVLTREYGSSSSTGNLILDAMMEKTPDAIAGFQNSGGMRADFPKGDITLGDVISTFPF
                     NNDLIEMDLTGRDLKSLMTHATNLTNGVLQVSKSVAVVYDSKKPLNQRLISFTINGKP
                     VEDNQTYRIATHSFCASGGDGFEAFLNGKNVKTIPGTTSAESIIDYFKNHKPVTPDLT
                     KRVMDVAK"
     gene            28175..29794
                     /locus_tag="PN977_000963"
     CDS             28175..29794
                     /locus_tag="PN977_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001357039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HDI6041026.1"
                     /translation="MKTVQRTLLAATLLTIFSVSVMAQDVTIIYTNDLHAHVDSYKVP
                     YIADGKRDIGGFANISTLVKQEKAKNKATFYFDAGDYFTGPYISSLTKGEAIIDIMNT
                     MPFDAVSIGNHEFDHGWDNALRQLSKANFPVLLGNVYHKESENPFWNKPYTILEKDGI
                     KIGIIGLHGVFAFNDTVSELSLQGLDNDNNNRFDKSSETLKNQGIEARDEVKYLQHYI
                     DELRDKVDLTVALVHEGVPARQSSIGNTDVRRALDKDIQTASKVKGLDILITGHAHVG
                     TPEPIKVGNTLILSTDSGGIDIGKLVLDVNPTARTHKMKSFELKTVYADEWIPDPTTQ
                     KVINGWNKKLADIVRQPVGESSIALTRAYGESSQLGNLFTDAMLVAAPTAQIALINSG
                     SLRADINAGTITFGDITSTFPFKNELTEMDLSGKDLRNLLEHGASLTNGILQMSKGAE
                     MRYTPQKPVGQRIVSFKINGEEIVDTNIYHVATTTFLALGGDGFLAFKEGKNVQVRAG
                     NNMSDVVIDYLKKGHKITPAQVNEMRVDVSK"
     gene            29872..30732
                     /locus_tag="PN977_000964"
     CDS             29872..30732
                     /locus_tag="PN977_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001765455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-specific channel-forming protein Tsx"
                     /protein_id="HDI6041027.1"
                     /translation="MCIKKHGIALSILLIPCIGNAQEIKSEEGWLHQSLNVIGRTDSR
                     FGPRLTNDLYPEYTVAGRKDWFDFYGYVDLPKFFGVGSHYDVGIWDEGSPLFTEIEPR
                     FSIDKLTGLNLAFGPFKEWFIANNYVYDMGDNQSSRQSTWYMGLGTDIDTGLPIKLSA
                     NIYAKYQWQNYGAANENEWDGYRFKIKYSIPLTHLFGGRLVYNSFTNFDFGSDLADKS
                     HNNKRTSNAIASSHILSLLYEHWKFAFTLRYFHNGGQWNAGEKVNFGDGPFELKNTGW
                     GTYTTIGYQF"
     gene            complement(30949..33381)
                     /gene="metL"
                     /locus_tag="PN977_000965"
     CDS             complement(30949..33381)
                     /gene="metL"
                     /locus_tag="PN977_000965"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000110794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase II"
                     /protein_id="HDI6041028.1"
                     /translation="MSVIAQAGAKGRQLHKFGGSSLADVKCYLRVAGIMAEYSQPDDM
                     MVVSAAGSTTNQLINWLKLSQTDRLSAHQVQQTLRRYQCDLISGLLPAEEADSLISAF
                     VSDLERLAALLDSGINDAVYAEVVGHGEVWSARLMSAVLNQQGLPAAWLDAREFLRAE
                     RAAQPQVDEGLSYPLLQQLLVQHPGKRLVVTGFISRNNAGETVLLGRNGSDYSATQIG
                     ALAGVSRVTIWSDVAGVYSADPRKVKDACLLPLLRLDEASELARLAAPVLHARTLQPV
                     SGSEIDLQLRCSYTPDQGSTRIERVLASGTGARIVTSHDDVCLIEFQVPASQDFKLAH
                     KEIDQILKRAQVRPLAVGVHNDRQLLQFCYTSEVADSALKILDEAGLPGELRLRQGLA
                     LVAMVGAGVTRNPLHCHRFWQQLKGQPVEFTWQSDDGISLVAVLRTGPTESLIQGLHQ
                     SVFRAEKRIGLVLFGKGNIGSRWLELFAREQSTLSARTGFEFVLAGVVDSRRSLLSYD
                     GLDASRALAFFNDEAVEQDEESLFLWMRAHPYDDLVVLDVTASQQLADQYLDFASHGF
                     HVISANKLAGASDSNKYRQIHDAFEKTGRHWLYNATVGAGLPINHTVRDLIDSGDTIL
                     SISGIFSGTLSWLFLQFDGSVPFTELVDQAWQQGLTEPDPRDDLSGKDVMRKLVILAR
                     EAGYNIEPDQVRVESLVPTHCEGGSIDHFFENGDELNEQMVQRLEAAREMGLVLRYVA
                     RFDANGKARVGVEAVREDHPLASLLPCDNVFAIESRWYRDNPLVIRGPGAGRDVTAGA
                     IQSDINRLAQLL"
     gene            complement(33384..34544)
                     /gene="metB"
                     /locus_tag="PN977_000966"
     CDS             complement(33384..34544)
                     /gene="metB"
                     /locus_tag="PN977_000966"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HDI6041029.1"
                     /translation="MTRKQATIAVRSGLNDDEQYGCVVPPIHLSSTYNFTGFNEPRAH
                     DYSRRGNPTRDVVQRALAELEGGAGAVLTNTGMSAIHLVTTVFLKPGDLLVAPHDCYG
                     GSYRLFDSLAKRGCYRVLFVDQGDEQALRAALAENPKLVLVESPSNPLLRVVDIAKIC
                     HLAREAGAVSVVDNTFLSPALQNPLALGADLVLHSCTKYLNGHSDVVAGVVIAKDPDV
                     VTELAWWANNIGVTGGAFDSYLLLRGLRTLVPRMELAQRNAQAIVKYLQTQPLVKKLY
                     HPSLPENQGHEIAARQQKGFGAMLSFELDGDEQTLRRFLGGLSLFTLAESLGGVESLI
                     SHAATMTHAGMAPEARAAAGISETLLRISTGIEDGEDLIADLENGFRAANKG"
     gene            34821..35138
                     /gene="metJ"
                     /locus_tag="PN977_000967"
     CDS             34821..35138
                     /gene="metJ"
                     /locus_tag="PN977_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015855437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="met regulon transcriptional regulator MetJ"
                     /protein_id="HDI6041030.1"
                     /translation="MAEWSGEYISPYAEHGKKSEQVKKITVSIPLKVLKILTDERTRR
                     QVNNLRHATNSELLCEAFLHAFTGQPLPDDADLRKERSDEIPEAAKEIMREMGINPET
                     WEY"
     gene            35198..35806
                     /locus_tag="PN977_000968"
     CDS             35198..35806
                     /locus_tag="PN977_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiX family permuted papain-like enzyme"
                     /protein_id="HDI6041031.1"
                     /translation="MKIRLLILSLLVSVPAFAWQPQTGDIIFQISRSSQSKAIQLATH
                     SDYSHTGMLVMRNKKPYVFEAVGPVKYTPLKQWIAHGEDGKYIVRRVEGGLNTEQQQK
                     LAQTAKRYLGKPYDFSFSWSDDRQYCSEVVWKVYQNALGMRVGEQQKLKEFDLSNPLV
                     QAKLKERYGKNIPLEETVVSPQAVFDAPQLTTVAKEWPLFSW"
     gene            complement(35867..36079)
                     /gene="rpmE"
                     /locus_tag="PN977_000969"
     CDS             complement(35867..36079)
                     /gene="rpmE"
                     /locus_tag="PN977_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HDI6041032.1"
                     /translation="MKKDIHPKYEEITASCSCGNVMKIRSTVGHDLNLDVCSKCHPFF
                     TGKQRDVATGGRVDRFNKRFNIPGSK"
     gene            36282..38480
                     /gene="priA"
                     /locus_tag="PN977_000970"
     CDS             36282..38480
                     /gene="priA"
                     /locus_tag="PN977_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HDI6041033.1"
                     /translation="MPVAHVALPVPLPRTFDYLLPEGMTVKAGCRVRVPFGKQQERIG
                     VVVSVSDVSELPLNELKVVVEVLDVEPVFTHSVWRLLLWAADYYHHPIGDVLFHALPI
                     LLRQGRPAANAPMWYWFATEQGQAVDLNSLKRSPKQQQALAALRQGKIWRDQVATLEF
                     NDAALQALRKKGLCDLASETPEFSDWRKNYAVSGERLRLNTEQATAVGAIHSAADTFS
                     AWLLAGVTGSGKTEVYLSVLENVLAQGKQALVMVPEIGLTPQTIARFRERFNAPVEVL
                     HSGLNDSERLSAWLKAKNGEAAIVIGTRSALFTPFKNLGVIVIDEEHDSSYKQQEGWR
                     YHARDLAVYRAHSEQIPIILGSATPALETLCNVQQKKYRLLRLTRRAGNARPAIQHVL
                     DLKGQKVQAGLAPALITRMRQHLQANNQVILFLNRRGFAPALLCHDCGWIAECPRCDH
                     YYTLHQAQQHLRCHHCDSQRPVPRQCPSCGSTHLVPVGLGTEQLEQTLAPLFPGVPIS
                     RIDRDTTSRKGALEQQLAEVHRGGARILIGTQMLAKGHHFPDVTLVALLDVDGALFSA
                     DFRSAERFAQLYTQVAGRAGRAGKQGEVVLQTHHPEHPLLQTLLYKGYDAFAEQALAE
                     RRMMQLPPWTSHVIVRAEDHNNQHAPLFLQQLRNLILSSPLADDKLWVLGPVPALAPK
                     RGGRWRWQILLQHPSRVRLQHIINGTLALINTIPDSRKVKWVLDVDPIEG"
     gene            38636..39661
                     /gene="cytR"
                     /locus_tag="PN977_000971"
     CDS             38636..39661
                     /gene="cytR"
                     /locus_tag="PN977_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CytR"
                     /protein_id="HDI6041034.1"
                     /translation="MKAKKQETAATMKDVALKAKVSTATVSRALMNPDKVSQATRNRV
                     EKAAREVGYLPQPMGRNVKRNESRTILVIVPDICDPFFSEIIRGIEVTAANHGYLVLI
                     GDCAHQNQQEKTFIDLIITKQIDGMLLLGSRLPFDASIEEQRNLPPMVMANEFAPELE
                     LPTVHIDNLTAAFDAVNYLYEQGHKRIGCIAGPEEMPLCHYRLQGYVQALRRCGIMVD
                     PQYIARGDFTFEAGSKAMQQLLDLPQPPTAVFCHSDVMALGALSQAKRQGLKVPEDLS
                     IIGFDNIDLTQFCDPPLTTIAQPRYEIGREAMLLLLDQMQGQHVGSGSRLMDCELIIR
                     GSTRALP"
     gene            39753..40712
                     /gene="ftsN"
                     /locus_tag="PN977_000972"
     CDS             39753..40712
                     /gene="ftsN"
                     /locus_tag="PN977_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsN"
                     /protein_id="HDI6041035.1"
                     /translation="MAQRDYVRRSQPAPSRRKKSTSRKKQRNLPAVSPAMVAIAAAVL
                     VTFIGGLYFITHHKKEESETLQSQKVTGNGLPPKPEERWRYIKELESRQPGVRAPTEP
                     SAGGEVKTPEQLTPEQRQLLEQMQADMRQQPTQLVEVPWNEQTPEQRQQTLQRQRQAQ
                     QLAEQQRLAQQSRTTEQSWQQQTRTSQAAPVQAQPRQSKPASTQQPYQDLLQTPAHTT
                     AQSKPQQAAPVARAADAPKPTAEKKDERRWMVQCGSFRGAEQAETVRAQLAFEGFDSK
                     ITTNNGWNRVVIGPVKGKENADSTLNRLKMAGHTNCIRLAAGG"
     gene            40805..41335
                     /gene="hslV"
                     /locus_tag="PN977_000973"
     CDS             40805..41335
                     /gene="hslV"
                     /locus_tag="PN977_000973"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HDI6041036.1"
                     /translation="MTTIVSVRRNGHVVIAGDGQATLGNTVMKGNVKKVRRLYNDKVI
                     AGFAGGTADAFTLFELFERKLEMHQGHLVKAAVELAKDWRTDRMLRKLEALLAVADET
                     ASLIITGNGDVVQPENDLIAIGSGGPYAQAAARALLENTELSAREIAEKALDIAGDIC
                     IYTNHFHTIEELSYKA"
     gene            41345..42676
                     /gene="hslU"
                     /locus_tag="PN977_000974"
     CDS             41345..42676
                     /gene="hslU"
                     /locus_tag="PN977_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019841241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase ATPase subunit"
                     /protein_id="HDI6041037.1"
                     /translation="MSEMTPREIVSELDKHIIGQDNAKRSVAIALRNRWRRMQLNEEL
                     RHEVTPKNILMIGPTGVGKTEIARRLAKLANAPFIKVEATKFTEVGYVGKEVDSIIRD
                     LTDAAVKMVRVQAIEKNRYRAEELAEERILDVLIPPAKNNWGQTEQQQEPSAARQAFR
                     KKLREGQLDDKEIEIDLAAAPMGVEIMAPPGMEEMTSQLQSMFQNLGGQKQKARKLKI
                     KDAMKLLIEEEAAKLVNPEELKQDAIDAVEQHGIVFIDEIDKICKRGESSGPDVSREG
                     VQRDLLPLVEGCTVSTKHGMVKTDHILFIASGAFQIAKPSDLIPELQGRLPIRVELQA
                     LTTSDFERILTEPNASITVQYKALMATEGVNIEFTDSGIKRIAEAAWQVNESTENIGA
                     RRLHTVLERLMEEISYDASDLSGQTITIDADYVSKHLDALVADEDLSRFIL"
     gene            42743..43669
                     /gene="menA"
                     /locus_tag="PN977_000975"
     CDS             42743..43669
                     /gene="menA"
                     /locus_tag="PN977_000975"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HDI6041038.1"
                     /translation="MTEQQISRTQAWLESLRPKTLPLAFAAIIVGTALAWWQGHFDPL
                     VALLALITAGLLQILSNLANDYGDAVKGSDKPDRIGPLRGMQKGVITQQEMKRALIIT
                     VVLICLSGLALVAVACHTLADFVGFLILGGLSIIAAITYTVGNRPYGYIGLGDISVLV
                     FFGWLSVMGSWYLQAHTLIPALILPATACGLLATAVLNINNLRDINSDRENGKNTLVV
                     RLGAVNARRYHACLLMGSLVCLALFNLFSLHSLWGWLFLLAAPLLVKQARYVMREMDP
                     VAMRPMLERTVKGALLTNLLFVLGIFLSQWAA"
     gene            43762..44247
                     /gene="rraA"
                     /locus_tag="PN977_000976"
     CDS             43762..44247
                     /gene="rraA"
                     /locus_tag="PN977_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001392924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E activity regulator RraA"
                     /protein_id="HDI6041039.1"
                     /translation="MKYDTSELCDIYQEDVNVVEPLFSNFGGRASFGGQIITVKCFED
                     NGLLYDLLEQNGRGRVLVVDGGGSVRRALVDAELARLAVQNEWEGLVIYGAVRQVDDL
                     EELDIGIQAMAAIPVGAAGEGIGESDVRVNFGGVTFFSGDHLYADNTGIILSEDPLDI
                     E"
     gene            44307..44981
                     /locus_tag="PN977_000977"
     CDS             44307..44981
                     /locus_tag="PN977_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6041040.1"
                     /translation="MQNIILLFIYLLLIVVNHFRYKFLLSGNTGEMILYFANVFFNPL
                     ALSFIIATIICITIKKRSSHSFIRGTCWLMGLFLIYSSYTVWERHSTWDYTFPGPGIT
                     VTVPSKQWVANIVKQGPILVTRDAHAILVIVAFSQNELGVHSIEELTATQNGTAEMHV
                     CDVKGFACAYQEGIQTMSDGKIRHAIFMTLLDNTNLIRLVAAIDPDYLDEYQEEVMKM
                     ILSAHQ"
     gene            45104..45730
                     /locus_tag="PN977_000978"
     CDS             45104..45730
                     /locus_tag="PN977_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6041041.1"
                     /translation="MVGEYIGSVFLGPLLLPVLVSGILCAFIKKTRNFASFTRGCCWV
                     LGILLLSKVGNTFRMFTPWQYTFENAAITVTIPNRHWNTVSISTNETIDIRSEDNSVF
                     ISAFRLPAGRSADDPLEALKKKQRDNLKDQYNEEAFQFHVCNAKHFTCKYQDVLINFD
                     GQQKRTISVYLEDTPRAVGIIALMAPDTADKYRQQAMEIMLSAKNAVK"
     gene            complement(45769..46014)
                     /gene="zapB"
                     /locus_tag="PN977_000979"
     CDS             complement(45769..46014)
                     /gene="zapB"
                     /locus_tag="PN977_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring assembly protein ZapB"
                     /protein_id="HDI6041042.1"
                     /translation="MTMSLEVFEKLEAKVQQAIDTITLLQMEIEELKEKNNSLSQEVQ
                     NAQHQREELERENNHLKEQQNGWQERLQALLGRMEEV"
     gene            46439..47284
                     /gene="glpF"
                     /locus_tag="PN977_000980"
     CDS             46439..47284
                     /gene="glpF"
                     /locus_tag="PN977_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol uptake facilitator protein GlpF"
                     /protein_id="HDI6041043.1"
                     /translation="MSQTSTLKGQCIAEFLGTGLLIFFGVGCVAALKVAGASFGQWEI
                     SVIWGLGVAMAIYLTAGVSGAHLNPAVTIALWLFACFDKRKVIPFIVSQVAGAFCAAA
                     LVYGLYYNLFFDFEQTHHIVRGSVESVDLAGTFSTYPNPHINFVQAFAVEMVITAILM
                     GLILALTDDGNGVPRGPLAPLLIGLLIAVIGASMGPLTGFAMNPARDFGPKVFAWLAG
                     WGNVAFTGGRDIPYFLVPLFGPIVGAIVGAFAYRKLIGRHLPCDICVVEEKETTTPSE
                     QKASL"
     gene            47307..48815
                     /gene="glpK"
                     /locus_tag="PN977_000981"
     CDS             47307..48815
                     /gene="glpK"
                     /locus_tag="PN977_000981"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HDI6041044.1"
                     /translation="MTEKKYIVALDQGTTSSRAVVMDHDANIISVSQREFEQIYPKPG
                     WVEHDPMEIWATQSSTLVEVLAKADISSDQIAAIGITNQRETTIVWEKETGKPIYNAI
                     VWQCRRTAEICEHLKRDGLEDYIRSNTGLVIDPYFSGTKVKWILDHVEGSRERARRGE
                     LLFGTVDTWLIWKMTQGRVHVTDYTNASRTMLFNIHTLDWDDKMLEVLDIPREMLPEV
                     RRSSEVYGQTNIGGKGGTRIPISGIAGDQQAALFGQLCVKEGMAKNTYGTGCFMLMNT
                     GEKAVKSENGLLTTIACGPTGEVNYALEGAVFMAGASIQWLRDEMKLINDAYDSEYFA
                     TKVQNTNGVYVVPAFTGLGAPYWDPYARGAIFGLTRGVNANHIIRATLESIAYQTRDV
                     LEAMQADSGIRLHALRVDGGAVANNFLMQFQSDILGTRVERPEVREVTALGAAYLAGL
                     AVGFWQNLDELQEKAVIEREFRPGIETTERNYRYAGWKKAVKRAMAWEEHDE"
     gene            48950..49960
                     /gene="glpX"
                     /locus_tag="PN977_000982"
     CDS             48950..49960
                     /gene="glpX"
                     /locus_tag="PN977_000982"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HDI6041045.1"
                     /translation="MRRELAIEFSRVTESAALAGYKWLGRGDKNTADGAAVNAMRIML
                     NQVNIDGTIVIGEGEIDEAPMLYIGEKVGTGRGDAVDIAVDPIEGTRMTAMGQANALA
                     VLAVGDKGCFLNAPDMYMEKLIVGPGAKGTIDLNLPLADNLRNVAAALGKPLSELTVT
                     ILAKPRHDAVIAEMQQLGVRVFAIPDGDVAASILTCMPDSEVDVLYGIGGAPEGVVSA
                     AVIRALDGDMNGRLLARHDVKGDNEENRRIGEQELARCKAMGIEAGKVLRLGDMARSD
                     NVIFSATGITKGDLLEGISRKGNIATTETLLIRGKSRTIRRIQSIHYLDRKDPEMQVH
                     IL"
     gene            50057..50803
                     /gene="fpr"
                     /locus_tag="PN977_000983"
     CDS             50057..50803
                     /gene="fpr"
                     /locus_tag="PN977_000983"
                     /EC_number="1.18.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP(+) reductase"
                     /protein_id="HDI6041046.1"
                     /translation="MADWVTGKVTKVQNWTDALFSLTVHAPVLPFTAGQFTKLGLEID
                     GERVQRAYSYVNSPDNPDLEFYLVTVPDGKLSPRLAALKPGDEVQVVSEAAGFFVLDE
                     VPDCETLWMLATGTAIGPYLSILQLGKDLDRFKNLVLVHAARYAADLSYLPLMQELEK
                     RYEGKLRIQTVVSRETAAGSLTGRIPALIESGELESAVGLPMNKETSHVMLCGNPQMV
                     RDTQQLLKETRQMTKHLRRRPGHMTAEHYW"
     gene            complement(50808..51236)
                     /gene="uspD"
                     /locus_tag="PN977_000984"
     CDS             complement(50808..51236)
                     /gene="uspD"
                     /locus_tag="PN977_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspD"
                     /protein_id="HDI6041047.1"
                     /translation="MAYKHIGVAISGNEEDALLVNKALELARHNDAHLTLIHIDDGLS
                     ELYPGIYFPATEDILQLLKNKSDNKLYKLTKNIQWPKTKLRIERGEMPETLLEIMQKE
                     QCDLLVCGHHHSFINRLMPAYRGMINKLSADLLIVPFIDK"
     gene            complement(51263..51562)
                     /locus_tag="PN977_000985"
     CDS             complement(51263..51562)
                     /locus_tag="PN977_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF406 domain-containing protein"
                     /protein_id="HDI6041048.1"
                     /translation="MKDVVDKCSTKGCAIDIGTVIDNDNCTSKFSRFFATREEAESFM
                     TKLKELAAAASSADEGASVAYKIKDLEGQVELDAAFTFSCQAEMIIFELSLRSLA"
     gene            complement(51774..52214)
                     /locus_tag="PN977_000986"
     CDS             complement(51774..52214)
                     /locus_tag="PN977_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HDI6041049.1"
                     /translation="MTIQQWLFSFKGRIGRRDFWIWIGLWFAGMLVLFSLAGKNLLDI
                     QTAAFCLVCLLWPTAAVTVKRLHDRGRSGAWAFLMIVAWMLLAGNWAILPGVWQWAVG
                     RFVPTLILVMMLIDLGAFVGTQGENKYGKDTQDVKYKADNKSSN"
     gene            52315..52914
                     /locus_tag="PN977_000987"
     CDS             52315..52914
                     /locus_tag="PN977_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiQ family protein"
                     /protein_id="HDI6041050.1"
                     /translation="MKPGCTLFFLLYSALTVTTTAHAQTPDTATTAPYLLAGAPTFDL
                     SISQFREDFNSQNPSLPLNEFRAIDSSPDKANLTRAASKINENLYASTALERGTLKIK
                     SIQMTWLPIQGPEQKAAKAKAQEYMAAVIRTLTPLMTKTQSQKKLQSLLTAGKNKRYY
                     TETEGALRYVVADNGEKGLTFAVEPIKLALSESLEGLNK"
     gene            53022..53789
                     /gene="tpiA"
                     /locus_tag="PN977_000988"
     CDS             53022..53789
                     /gene="tpiA"
                     /locus_tag="PN977_000988"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HDI6041051.1"
                     /translation="MRHPLVMGNWKLNGSRHMVHELVSNLRKELAGVAGCAVAIAPPE
                     MYIDMAKREAEGSHIMLGAQNVDLNLSGAFTGETSAAMLKDIGAQYIIIGHSERRTYH
                     KESDELIAKKFAVLKEQGLTPVLCIGETEAENEAGKTEEVCARQIDAVLKTQGAAAFE
                     GAVIAYEPVWAIGTGKSATPAQAQAVHKFIRDHIAKVDANIAEQVIIQYGGSVNASNA
                     AELFAQPDIDGALVGGASLKADAFAVIVKAAEAAKQA"
     gene            complement(53844..54599)
                     /gene="cdh"
                     /locus_tag="PN977_000989"
     CDS             complement(53844..54599)
                     /gene="cdh"
                     /locus_tag="PN977_000989"
                     /EC_number="3.6.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol diphosphatase"
                     /protein_id="HDI6041052.1"
                     /translation="MKKAGLLFLVMIVIAVVAAGIGYWKLTGEESDTLRKIVLEECLP
                     NQQQNQNPSPCAEVKPNAGYVVLKDLNGPLQYLLMPTYRINGTESPLLTDPSTPNFFW
                     LAWQARDFMSKKYGQPVPDRAVSLAINSRTGRTQNHFHIHISCIRPDVREQLDNNLAN
                     ISSRWLPLPGGLRGHEYLARRVTESELVQRSPFMMLAEEVPEAREHMGSYGLAMVRQS
                     DNSFVLLATQRNLLTLNRASAEEIQDHQCEILR"
     gene            complement(54706..55695)
                     /gene="sbp"
                     /locus_tag="PN977_000990"
     CDS             complement(54706..55695)
                     /gene="sbp"
                     /locus_tag="PN977_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter
                     substrate-binding protein Sbp"
                     /protein_id="HDI6041053.1"
                     /translation="MNKWGVGLTFLLAATSVMAKDIQLLNVSYDPTRELYEQYNKAFS
                     AHWKQQTGDNVVIRQSHGGSGKQATSVINGIEADVVTLALAYDVDAIAERGRIDKEWI
                     KRLPDNSAPYTSTIVFLVRKGNPKQIHDWNDLIKPGVSVITPNPKSSGGARWNYLAAW
                     GYALHHNNNDQAKAQDFVRALYKNVEVLDSGARGSTNTFVERGIGDVLIAWENEALLA
                     ANELGKDKFEIVTPSESILAEPTVSVVDKVVEKKGTKEVAEAYLKYLYSPEGQEIAAK
                     NYYRPRDAEVAKKYENAFPKLKLFTIDEEFGGWTKAQKEHFANGGTFDQISKR"
     gene            complement(56015..56977)
                     /gene="pfkA"
                     /locus_tag="PN977_000991"
     CDS             complement(56015..56977)
                     /gene="pfkA"
                     /locus_tag="PN977_000991"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014072359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HDI6041054.1"
                     /translation="MIKKIGVLTSGGDAPGMNAAIRGVVRSALTEGLEVMGIYDGYLG
                     LYEDRMVQLDRYSVSDMINRGGTFLGSARFPEFRDENIRAVAIENLKKRGIDALVVIG
                     GDGSYMGAMRLTEMGFPCIGLPGTIDNDIKGTDYTIGFFTALSTVVEAIDRLRDTSSS
                     HQRISVVEVMGRYCGDLTLAAAIAGGCEFVVVPEVEFSREDLVNEIKAGIAKGKKHAI
                     VAITEHMCDVDELAHFIEKETGRETRATVLGHIQRGGSPVPYDRILASRMGAYAIDLL
                     LAGYGGRCVGIQNEQLVHHDIIDAIENMKRPFKGDWLDCAKKLY"
     gene            complement(57158..58060)
                     /gene="fieF"
                     /locus_tag="PN977_000992"
     CDS             complement(57158..58060)
                     /gene="fieF"
                     /locus_tag="PN977_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709720.2"
                     /note="FieF, a metal efflux transporter, is a member of
                     the CDF (cation diffusion facilitator) family of
                     transporters; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family cation-efflux transporter FieF"
                     /protein_id="HDI6041055.1"
                     /translation="MNQSYGRLVSRAAIAATAMASLLLLIKIFAWWYTGSVSILAALV
                     DSLVDIGASLTNLLVVRYSLQPADDNHSFGHGKAESLAALAQSMFISGSALFLFLTGI
                     QHLVSPTPMTDPGVGVIVTIVALICTIILVSFQRWVVRRTQSQAVRADMLHYQSDVMM
                     NGAILLALGLSWYGWHRADALFALGIGIYILYSALRMGYEAVQSLLDRALPDEERQEI
                     IDIVTSWPGVSGAHDLRTRQSGPTRFIQIHLEMEDSLPLVQAHMVADQVEQAILRRFP
                     GSDVIIHQDPCSVVPREGKRSMLS"
BASE COUNT        14699 a        15280 c        14553 g        13750 t
ORIGIN      
        1 caatcaccag cggacgctga tgtgacacac gataattcac cagtgcacta aacagacgtt
       61 ccagcgcctc ttcactatcc agcagtacgc cgcccagttt gataattaat ggattcatca
      121 ttgcaccctt aaataagaga ctgcgtttcc gcatagccga aacgaatatt ggcgcactgt
      181 accgcttgtg ccgccgcgcc tttcagtaag ttgtcttcgg tcgtcacaat aatcagatgc
      241 tcgccctgaa cggcaaaccc gatatcgcaa aatggcagcc caacgacatt tttcagcgcc
      301 ggaacgcctt tgtcatacag ccgcaccagc ggtttatggg catacgcctg ctgtaacact
      361 tgcgcgactt gcgcctgggt cacacccgat ttcaggcggc aggtaatggt ttcgagaatg
      421 ccgcgcggga aattgcccag atgcggggtg aagataacgt cagcaccgag atgtgtggcg
      481 atctctggtt gatggcgatg ggtaaagacg ccatacggtt gcaggctaac ttcacaaaaa
      541 ctatttgaaa tcgctgcctt acgtcctgca ccgctcacgc cgctggtagc gttgatcacc
      601 ggccactgaa tgaggtcaag aagatcggca tcaatcaacg gtttcagcgc cagctgtgct
      661 gccgtcggat aacagcctgg caccgcaatc aagtttgctt cttttaattt attaccgcac
      721 cactccgcca gaccgtaggc tgcctgttcc agcagttccg ggtattgatg ggtaaagccg
      781 taatattttt catagaaggc ggcgtcgtta acacgaaacg cgccggaaag gtcgaacacc
      841 acgcagccgg cttcaagaaa ttgcggcgct aaatcgtggc taacttcatg ggcggtggcg
      901 agaaacacca cgtccacccc tgggctaaac tcgctgatat ccgacatcgg ctgcaacggc
      961 agatcaacga tgccttttag ctgcggatgc aaatcggaga ttaactttcc cgcatcattg
     1021 ctttgcgctg aaacagtcaa agcggttatg ttcatatgcg gatggcgatt tacataggtc
     1081 actagctctg cgccagcgta gccgctggca cccacaatca gcgtattcaa catcggggct
     1141 attcaccttc ttatgtctgg ttgccaggtt aaacgtaaaa cattcacctt acggctggtg
     1201 ggttttatta cgctcaacgt tagtgtattt ttattcataa atactgcatg aatattgata
     1261 ctatcatgac cagaggtgtg tcaacaatga aaaacaaatt accgccattt atcgagattt
     1321 accgtgctct gattgccaca ccttcaataa gcgccacgga agaggcactc gatcaaagca
     1381 atgcagattt aatcactctg ctggcggact ggtttaaaga tttaggcttc aatgtggaag
     1441 tgcagcctgt gccaggaact cgcaacaaat ttaatatgct ggcaagtacc ggacaggggg
     1501 ctggcggttt gttgctggcg gggcataccg atacggtgcc atttgatgac ggacgctgga
     1561 cgcgcgatcc gtttacactg acggagcatg acggcaagct ttacggctta ggcaccgccg
     1621 acatgaaagg cttttttgcg tttatccttg atgcgctacg cgatgtcgac gtcacgaagc
     1681 ttgcaaaacc gctctacatt ctggcgactg ccgatgaaga aaccagtatg gccggagcgc
     1741 gttattttgc cgaaactacc gccctgcgcc cggattgcgc catcattggc gagccaacgt
     1801 cgctgcaacc ggtacgcgcc cataaaggtc atatctctaa cgccatccgt attcagggcc
     1861 agtcgggcca ctccagcgat ccagcacgcg gagttaacgc tatcgaactg atgcacgacg
     1921 ccatcgggca tattttgcaa ttgcgcgata agctgaaaga acgttatcac tatgaagcgt
     1981 ttaccgtgcc ataccccacg ctcaacctcg ggcatattca cggtggcgac gcttctaacc
     2041 gtatttgtgc ttgctgtgag ttgcatatgg atattcgtcc gctgccgggc atgacactca
     2101 atgaacttaa tggcctactc aacgatgcgc tggctccggt gagcgaacgc tggccgggtc
     2161 gtctgacggt cgacgagctg catccgccga tccctggcta tgaatgcccg ccaaatcatc
     2221 aactggttga agtagttgag aaattgctcg gagcaaagac cgaagtggtg aattactgta
     2281 ccgaagcgcc gtttattcaa acgttatgcc cgacgctggt tctgggtcct ggctcaatta
     2341 atcaggctca tcaacctgat gaatatctgg aaacacggtt tatcaagccc acccgcgaac
     2401 tgatcaccca ggtgattcac catttttgct ggcattaaaa cgtaggccaa acaatgtcgg
     2461 atgcgatgct tgcgcatctt atccgaccta cacctttggt gttactcgag gcgatttttt
     2521 aacattccca taagttacgc ttatttaaag cgtcgtgaat ttaatggcgt aaattcctgc
     2581 tatttattcg tttgctgaag cgatttcgca gcatttgacg tcaccgcttt tacgtggctt
     2641 tataaaagac gacgaaaagc aaagcccgag catattcgcg ccaatgcgac gtgaaggata
     2701 cagggctatc aaacgataag atggggtgtc tggggtaata tgaacgaaca atattccgca
     2761 ttgcgtagta atgtcagtat gctcggcaaa gtgctgggag aaaccatcaa ggatgcgtta
     2821 ggagaacaca ttcttgaacg cgtagaaact atccgtaagt tgtccaaatc ttcacgcgct
     2881 ggcaatgatg ctaaccgcca ggagttgctc accaccttac aaaatttgtc gaacgacgag
     2941 ctgctgcccg ttgcgcgtgc gtttagtcag tttctgaacc tggccaacac cgccgagcaa
     3001 taccacagca tttcgccgaa aggcgaagct gccagcaacc cggaagtgat cgcccgcacc
     3061 ctgcgcaaac tgaaaaacca gccggaactg agcgaagaca ccataaaaaa agcagtggaa
     3121 tcgctgtcgc tggaactggt cctcacggct cacccaaccg aaattacccg tcgtacactg
     3181 atccacaaaa tggtggaagt gaacgcctgc ttaaaacagc tcgataacaa agatatcgct
     3241 gactacgaac acaaccagct gatgcgccgc ctgcgccagt tgatcgccca gtcatggcat
     3301 accgatgaaa tccgtaagct gcgtccaagc ccggtagatg aagccaaatg gggctttgcc
     3361 gtagtagaaa acagcctgtg gcaaggcgta ccgaattacc tgcgcgaact gaacgaacaa
     3421 ctggaagaga atctcggcta caaactgccc gtcgaatttg ttccggtccg ttttacctcg
     3481 tggatgggcg gtgaccgcga cggcaacccg aacgtcactg ccgatatcac ccgccacgtc
     3541 ctgctgctca gtcgctggaa agccaccgat ctgttcctga aagatattca ggtgctggtt
     3601 tctgaactgt cgatggttga agcgacccct gaactgctgg cgctggttgg cgaagaaggt
     3661 gccgcagaac cgtatcgcta tctgatgaaa aacctgcgtt ctcgcctgat ggcgacacag
     3721 gcatggctgg aagcgcgcct gaaaggcgaa gaactgccaa aaccagaagg cctgctgaca
     3781 caaaacgaag aactgtggga accgctctac gcttgctacc agtcacttca ggcgtgtggc
     3841 atgggtatta tcgccaacgg cgatctgctc gacaccctgc gccgcgtgaa atgtttcggc
     3901 gtaccgctgg tccgtattga tatccgtcag gagagcaccc gccataccga agcgctgggc
     3961 gagctgaccc gctacctcgg tatcggcgac tacgaaagct ggtcagaggc cgacaaacag
     4021 gcgttcctga tccgcgaact gaactccaaa cgtccgcttc tgccgcgcaa ctggcaacca
     4081 agcgccgaaa cgcgcgaagt gctcgatacc tgccaggtga ttgccgaagc accgcaaggt
     4141 tccattgccg cctacgtgat ctcgatggca aaaacgccgt ccgacgtact ggctgtccac
     4201 ctgctgctga aagaagcggg tatcgggttt gcaatgccgg ttgctccgct gtttgaaacc
     4261 ctcgatgacc tgaacaacgc caacgatgtc atgacccagc tgctcaatat cgactggtat
     4321 cgcggcctga ttcagggcaa acagatggtg atgattggct attccgactc agcaaaagat
     4381 gcgggcgtga tggcagcttc ctgggcgcaa tatcaggcac aggatgcatt aatcaaaacc
     4441 tgcgaaaaag cgggtattga gctgacgttg ttccacggtc gcggcggttc cattggtcgt
     4501 ggcggcgcac ctgctcatgc ggcgctgctg tcacaaccgc caggtagcct gaaaggtggc
     4561 ctgcgcgtga ctgaacaggg cgagatgatc cgctttaaat atggtctgcc agaaatcacc
     4621 gtcagcagcc tgtcgctgta caccggggcg attctggaag ccaacctgct gccaccgcct
     4681 gagccgaaag agagctggcg tcgcattatg gatgaactgt cagtcatctc ctgcgatctc
     4741 taccgtggct acgtacgtga aaacaaagat tttgtgcctt acttccgctc cgctacgccg
     4801 gaacaagaat tgggcaaact gccgttgggt tcacgtccgg cgaaacgtcg cccaaccggc
     4861 ggcgtcgagt cgctgcgcgc cattccgtgg atttttgcct ggacgcaaaa ccgcctgatg
     4921 ctccccgcct ggctgggtgc aggtacggcg ctgcaaaaag tggtcgaaga tggcaagcag
     4981 agcgaactgg aagccatgtg ccgcgattgg ccattcttct cgacgcgtct cggcatgctg
     5041 gagatggtct tcgccaaagc agacctgtgg ctggcggaat actatgatca acgtctggta
     5101 gacaaagcac tgtggccgtt aggtaaagag ttacgcaatc tgcaagaaga agacatcaaa
     5161 gtggtgctgg cgattgccaa cgattcccat ctgatggccg atctgccgtg gattgcagag
     5221 tctattcagc tacggaatat ttacaccgac ccgctgaacg tattgcaggc cgagttgctg
     5281 caccgctccc gccaggcaga aaaagaaggc catgagccgg atcctcgcgt cgagcaggcg
     5341 ttaatggtca ctattgccgg gattgcggca ggtatgcgta ataccggcta atcttcctct
     5401 cctgcaaacc ctcgtgcttt tgcgcgaggg ttttctgaaa tacttctgtt ctaacacctt
     5461 cgttttcaat atatttctgt ctgcatttta ttcaaattct gaatacacct tcagatatcc
     5521 ttaaggaatt gtcgttacat tcggcgatat ttttttcaag acaggttctt actatgcatt
     5581 ccacagaagt ccaggctaaa ccccttttta gctggaaagc cctgggttgg gcactgctct
     5641 acttttggtt tttctctact ctgctacagg ccattattta catcagtggt tatagtggca
     5701 ctaacggcat tcgcgactcg ctgttattca gttcgctgtg gttgatcccg gtattcctct
     5761 ttccgaagcg gatcaaaatt attgccgcag tgatcggcgt ggtgctatgg gcggcctctc
     5821 tggcggcgct gtgctactac gtcatctacg gtcaggagtt ctcgcagagc gttctgtttg
     5881 tgatgttcga aaccaacacc aacgaagcta gcgagtattt aagccagtat ttcagcctga
     5941 aaattgtgct tatcgcgctg gcctatacgg cggtggcagt tctgctgtgg acacgcctgc
     6001 gcccggtcta tattccaaag ccgtggcgtt atgttgtctc ttttgccctg ctttatggct
     6061 tgattctgca tccgatcgcc atgaacacca tcatcaaagg caaaccgatt gaaaaaacgc
     6121 tggatagtct ggcatcgcga atggaacccg cagcaccatg gcaatttatt tccggctact
     6181 accagtaccg ccagcaactt aactcgctga ccaaattact caacgaaaac aatgcgctgc
     6241 cgccgctggc taatttcaaa gatgaatcgg gtaacgaacc gcgcacctta gtgctggtga
     6301 ttggcgagtc gacccagcgt ggacgcatga gtctgtacgg ttatccgcgt gaaaccacgc
     6361 cggagctgga tgcgctgcat aaaaccgatc cgaatctgac cgtgtttaat aacgtggtga
     6421 cgtctcgtcc gtacaccatt gaaatcctgc aacaggcgct gacctttgcc aatgaaaaga
     6481 acccggatct gtatctgacg cagccgtcgc tgatgaacat gatgaaacag gcgggttata
     6541 aaaccttctg gatcaccaac cagcagacga tgaccgcccg caataccatg ctgacggtat
     6601 tttcgcgcca gaccgacaag cagtactaca tgaaccagca acgtacacag agtgcgcgtg
     6661 aatacgacac taacgtgctg aagccgttcc aggaagtgct gaaggaccct gcgccgaaga
     6721 aactgatcat cgttcatctg ctgggtacgc atatcaaata caaataccgc tacccggaaa
     6781 atcagggcaa gtttgatggc aataccgatc atgtcccgcc ggggttaaac gcggaagagc
     6841 tggagtcata taacgattat gacaacgcta acctgtataa cgatcatgtg gttgccagcc
     6901 tgattaaaga ctttaaagcg gcagacccga acggattcct ggtttacttc tctgaccacg
     6961 gtgaagaggt ttacgacacg ccgccgcata aaacccaggg gcgtaatgag gacaacccga
     7021 cgcgccacat gtacaccatt ccgttcctgc tgtggacgtc ggaaaaatgg caagcgactc
     7081 atccgcgtga tttctcacag gatgttgatc gtaaatacag cctggcggaa ttgatccaca
     7141 cctggtcaga tttagcgggc ttatcttacg acggttacga cccaacccgt tcagtggtga
     7201 atccgcagtt caaagaaact acccgctgga ttggtaaccc gtacaagaaa aacgcgctga
     7261 tcgattacga cacgctgccg tatggcgacc aggtaggtaa tcagtaaatg acctttgtat
     7321 cgcgtctggc gctatttgcc ggatgcgata ctgtcgcaat gtttacatcg aactcccctc
     7381 aggccacact tttcaggtgg ccttttttca taatgccagt tactgttttc ccgtctacat
     7441 acctaaggga ataacatgta tcacgacgtc agctatctgc tctcccgcct gatcaacggc
     7501 ccgctgtccc tgcgccagat ttactttgcc agcagtaacg gcccggtgcc cgatcttgcg
     7561 tatcaggtcg attttccacg actggaaatt gtgctggaag gtgagtttgt tgataccggc
     7621 gctggagcaa cgttagttcc cggcgatgtg ctgtacgttc ccgctggtgg ctggaatttt
     7681 ccacaatggc aagcccccgc cactaccttt agcgtgctgt ttggcaaaca gcaactcggc
     7741 ttcagcgtcg tgcaatggga tggcaaacaa tatcaaaatc tggcgaagca acacgtcgcc
     7801 cgacgcggcc cgcgcattgg ttcttttctg ctacaaacgc tcaatgaaat gcaaatgcag
     7861 ccgcaggagc agcaaacggc aaggcttatc gtcgccagcc tgcttagcca ctgccgcgat
     7921 ttgctcggca gccaaatcca gaccgcctca cgtagccagg ctctattcga agctattcgc
     7981 gattatatcg acgaacgcta tgcctccgcg cttacccgcg aatctgtcgc acaggcattc
     8041 tatatttcgc caaattacct ctcgcacctg tttcaaaaaa cgggggccat tggttttaac
     8101 gaatacctga atcacacgcg actggagcac gctaagacgt tattgaaagg ctatgacctg
     8161 aaggtaaaag aagtggcgca cgcctgcggc tttgtcgaca gcaactactt ctgccgtctg
     8221 tttcgtaaaa ataccgaacg ctcaccgtcg gaatatcgtc ggcagtacca cagccaactg
     8281 acagaaaaac cgactactcc agaataagat gggtttgctg cggcgcagaa agcactttgc
     8341 gtactgcgct catcacccgc tgcggctcac gcaggaaagc gtagatgctg cattgcacgt
     8401 agcggcaatg ttcaaatcgc tcggcaccgg caagctcgat atccgtaatc aacagagcaa
     8461 catccgcccg acggatatcc tcgtccgcta aacgattctc cgttcccagc gcgccctgag
     8521 tttcaatgct gacgccccac ttctctaaca ggcacaactt ttccagccgt tccgccgcca
     8581 tataagtatg cgccacgccg ctgacgcagg cggttactgc caccaggtat gccattttaa
     8641 cctcccacgg taacctgaaa tccggcccgc tctgccattt cacgcatcgt tgccacatcg
     8701 gctgacgacg gcgcagacac ctctttcatc gaccatgttt tccccagcag gcggtatttc
     8761 ggttcgccgt actgatgaaa cggtaacaga tggatctgcc tgatattcag cgggatcagc
     8821 acgtccagcg cctgctgcat attctcccgg ctgagcgtga aaccagggat cagcggtaaa
     8881 cgcgggatca cgttgacgcc ctcactcacc agcaaacgca gattctccag cacgcgtggc
     8941 aggttcatct tcaccacatc ccgcgcctga gtcgcgtcca taatttttaa atcgaacaac
     9001 acttcatcgc acaatttcgc cagcggtaac agcttgctgg ctggcgcgtc tccggcagtt
     9061 tcgatggcgc atgacacacc ccacaaccgc agccgctgta aaaaaagagt agcaaactcc
     9121 gcctgcatta acacttcgcc gccagaaagc gtgacgccgc cgccggacgt gcgaaagaaa
     9181 atgtcatctt tcatcacttc ccgttccaga gcgtcaaggc tgatatcgcg accaatccgt
     9241 tcaaacgcac cggaggggca ttcatccgca tcacgcaaac atttcgcaca gtgcagacat
     9301 ttcgcctctc tgcgtacagt ctggattttg ccggagatcg actccggatt agcacaccac
     9361 gggcaaagat gtggacagcc tttaaaaaag accaccgtac gaatgccctc gccgtcattc
     9421 agtgaataac gctgaatgtt gaaaatgcgc gccacatcat tgcggcgcgt ttccacaacg
     9481 ttacagctga tgcgctgtcc ggcggatgat gtcatcctgg atctccttcg acaattcgac
     9541 aaagaaggcg ctgtatccgg caacgcgcac caccagcccg gcataatctt gtgggcgctg
     9601 ttgcgcttcc cgcaacgtgt cggcgttcac cacgttaaac tgaatatgct gcaacttaag
     9661 ctgggtaaac gcccgtaaga agtcggccag tttgcgtaat cccgcttcac cttccagggt
     9721 cgccggagtg aatttcacgt tcagcaacgt accgttagac agcagcgtat tatcgagctt
     9781 actgactgac ttcagtaccg ccgttggccc ttgtgcgtcc tggcccagca tgggtgacaa
     9841 gccgccgtct gccagctgtt ctccggcaaa acgaccgtct ggcgtcgcgc caaccaccga
     9901 tcccaacggg acgtgagcag aaacggtata cgatcccggc gtgaagtagc cgccgcgcgg
     9961 gttctggtat ttttccactt ctttgcagta gtggcgtaac agttcggcgc tgatgttatc
    10021 cacctcgtcg atatcgttac cgtatttctc gaagcggtta attaagcgag cacggacttt
    10081 ttcgccttct ggcgttgcga agttggcttt taataccgac agcaattcgt caaaacttaa
    10141 acgctgttgc tcaaaaacca tacctttcaa cgcatggaga gaatcgctca ggttggcgat
    10201 accgatcccc tgtacgccgg agaagttata acgcgcaccg ccatcggtaa tatcgcggcc
    10261 tttttccaga caatcgctga taaacgatga gagcagcggt acaggtgccc agtcacggtg
    10321 accgatatcg caaatattgc tgccctcaac catcagggtg atgtagtggc tgatcttggc
    10381 gcggatctgc tccagtaaac cttcataagt cagcgcagca ttgccttcat tttcatgcag
    10441 gcagatttcc atcactttca gcagattaaa catcgcgatg tcatgcaagc cgtaggttct
    10501 gccgggaata gataattcca cacagcccac tacggaatag tcgcgtgcat cttccagcga
    10561 aacgccacgg ttgaggaacg ctggcaccac tacttcatcg ttaaagattt gcggaatacc
    10621 ggtgccgagg cgaatggttt cggcggtttt catcaggaac ggcgtgtcga tcaaggcgtt
    10681 agtgcgcacc cccaggttcg gttgcggtaa ttgcacgctt tgataggcgt caaggcaaag
    10741 gaacgaaagc acgttcaccg cgctacgtcc gttctcggtt aacccgccga gcagtgcggt
    10801 atagccggtc gggaaaccgg cgaaataacg cgcgctactg gtggagcgca acagcacgat
    10861 gtcgttgcat ttcacccata aagattcgag cagttctttc aggaacgccg gatcttcgcc
    10921 ctgggttaat gatgcctgat agaacggcaa catatactgg tcgaagcgcc ctaaagacag
    10981 cgaactggcg ttggattcgt attgcagaat gatgttcatg taccagaata actggcacgc
    11041 ctgccagaac gtctgcggct tatgttcggc gttatggcgg gagatctccg caatagtcag
    11101 cagttcttcg cgacgctggc catcagtgca gcttgccgcc atcgtttccg ccagttcggc
    11161 gtaacgcaaa atatgtttct gcgaggcttc cagcagtaac agcgctgcct gataaaagtg
    11221 attctccggc tgttgctgac aatgttgctg catctgtgcc accagctccc ccagcccgtg
    11281 attcagcagg cgcgggtaat caataataat gtgcccctgg cctttatccg tctggttgat
    11341 gctgaaaatc tgcgtgctgg tcgcggcttt tacttcatcc gtcatctgcc cgttaatgaa
    11401 atctttcatc gaacgttttt cccagtacgg gaacaactct tcgcggtaga tacgtttatc
    11461 ttcttcgctg atagcaaagc ggtcctgcgg acgcgtcggg aattgatcca gctctttcag
    11521 cagccagtaa gggtccattt ccggcgacat aatcccggcg cgtggtttta cggtgcggtt
    11581 accggcaatc agttcttcat cacgaatcga aatttcaacg tgttcaagga tatacgctgt
    11641 tgctttcgcc cggcgcaata tcaccggttc gccttcggtt tgccgatggc tggcggtata
    11701 aagcagcgcc cgctccagcg agatttcacg ggtattggca aacagtgcag ttttgaggcg
    11761 agagatacga ttcgtcatga ggaactcctg tgggaaagcg aacccgccct tacgggcggg
    11821 ataagaactt aagcggtttg tgccaggtga gcttcaattt tgctcataat ggcatcagcg
    11881 cgttttaccg cgtcgctgat gttgacgcgc acgatggtct tgccagcaaa acgctcttca
    11941 aacttgatgc cgatatcttt ggtcagaatc accatatcgg cgctcgccac gtcttccgca
    12001 gtcagttcat tttccagacc aatcgagccc tgggtttcta ctttcacttc ccagcctttc
    12061 gctttcgcgg cactttccag cgcctctgcc gccatatagg tatgggcaac accagatgga
    12121 cacgcggtta ctgcaataat tttcgtcatg gctcgttcct caattaatta atttcaaaat
    12181 ccaaatccag gtcgtcttct ttttcatcag tcgaagaccc atttttacgc gccagacttt
    12241 tcagcacgtt cacacaaaca gccgtcacca ctgctccgac agccactgct gcgatataac
    12301 ccagcttgcc ttcaaccacc ggcagcacaa tcagaccacc ccagcctgca tagcactgcg
    12361 caccgaccag cgccgcagtt actgcaccac aaactgaacc gatcatgatg gaaggaataa
    12421 cacgcagcgg atcggcagcg gcgaaaggaa tcgccccttc agtaacccca acacagccca
    12481 tcaccagtgc cgccttgccg gtttcgcgct cttctgcgga gaaattttta cgaccaatca
    12541 gcgtcgccag ccccatcccc agcggtggaa cacaaatccc aacagcggca atagccacca
    12601 cggtataaac accctgagca acgcaaatca gcatgaaggc ataggccact ttgttaaccg
    12661 ggccgcccat atcgaacgcc agcatcagac ccatgatcac cgccagcata acaatgctgc
    12721 cctgctgcat cccctgaagc cactgagtca ggctgttggt caacgcccct acaggctcgc
    12781 ccagccccca catcatgacg ccagcggtaa tcagtgtgcc gacgatagga atgatgaaga
    12841 ttggcatcac cgagcgcaga actttatgca ccggaatttt cttcaggtaa tgcaccacga
    12901 tgccgccgat aatcccggcg atcagtgcac caaagaagcc cgcaccaaag ctgttaccaa
    12961 cccaggcacc gatagcgcac ggagccagcg cagagcgttc tgcaatggaa taaccgatgt
    13021 aagcggcgag gaaaggcacc atcagcgtca aacctgcaac gccgatatca aacagttttt
    13081 tcagattcgg atcggcgacg gcatccggca ctgcgccttt gccatacaac atgacggaaa
    13141 ccgccagcaa aataccgccc gataccacga agggaatcat gtgtgaaacg cccgtcatta
    13201 aatgctgacg agtatttttc aggatctgca ccaactcatt cataagctgc tcccgggctt
    13261 gcgaaagccc atgtccatat ttttgatgga agaaacaata ggcaatcgca ggcggagcaa
    13321 acagtggata aaaaggccat ttactggact tttgtaatat cagtacaaaa atgcgatccg
    13381 cctcataact tgcgataacc cggaagcatg ccgcaaaacg gcgtcttgtg agacgcgtcg
    13441 caaattggcg gttacattac atttgtccag cttttggcag atttgtcaca tggcaggccg
    13501 ttgccgtgcg ctcttatcct gtaaacagaa aaccgtaccg ggagagaggc aatggccctg
    13561 attgtggaat ttatttgtga gctacctaac ggcgtacatg cgcgtccggc aagccacgtt
    13621 gaaacgctgt gtaatacttt ttcatcacaa attgagtggc ataacctgcg cactgaccgc
    13681 aagggcaacg ccaaaagcgc ccttgcgctg attggcaccg atacgctggt gggcgataac
    13741 tgccagttac tgatttccgg ggccgacgaa caggaagcgc accagcgttt aagccaatgg
    13801 ctgcgcgatg aattccccca ctgcgacgca ccgctggcgg aagtaaaatc tgacgaactg
    13861 gaaccactgc cggtttcact gaccaatctg aatccgcaaa ttatccgtgc ccgcaccgtg
    13921 tgcagcggta gcgcaggcgg cattctgacg ccgatctctt ctttagatct caatgcgctg
    13981 ggtaatcttc ccgcagccaa aggcgttgac gccgaacaat ccgcactgga aaacggtctg
    14041 acgctggtac tgaaaaacat agagtttcgt ctgctggata gcgacggtgc taccagcgcg
    14101 attctggaag cgcaccgatc cctggctggc gatacttccc tgcgcgaaca tttactggca
    14161 ggtgtcagcg ccggattaag ctgcgccgaa gcgattgttg ccagtgcgaa tcacttttgc
    14221 gaagagttcg cccgttccag cagcagctac ctgcaagaac gtgccctgga cgtacgcgac
    14281 gtctgcttcc agttactcca gcaaatctac ggtgagcaac gcttcccggc accgggcaaa
    14341 ctgacgcagc ccgccatttg tatggctgat gaactgaccc ccagccagtt cctcgaactg
    14401 gataaaaatc acctcaaagg attgttgctc aaaagcggcg gcaccacctc acatacggtg
    14461 atccttgccc gttcgttcaa cattccgacg ctggttggtg tggatattga tgccctcact
    14521 ccgtggcagc atcaaacgat ttatatcgac ggcaacgctg gggcgattgt ggttgagcca
    14581 ggggaatccg tggcccgtta ttatcagcaa gaagcccgcg tacaggacgc tctgcgtgag
    14641 caacagcgtg tctggctgac ccaacaagcc cgtaccgctg acggtatccg cattgaaatt
    14701 gctgctaaca tcgctcactc cgtggaagcg caggccgcat tcggcaatgg tgcggaaggc
    14761 gttggtttat tccgcactga aatgctctat atggatcgca ccagcgcacc gggcgaaagc
    14821 gagctgtaca acattttttg tcaggcgctg gagtccgcca acggacgcag cattattgtg
    14881 cgcactatgg atattggcgg tgacaaaccc gttgattatc tgaacattcc cgcagaggca
    14941 aacccgttcc tcggttatcg cgccgtgcgt atttatgaag agtacgcgtc gttatttacc
    15001 acacaactac ggtcgattct ccgtgcctcc gctcacggca gcctgaaaat catgatcccg
    15061 atgatctcct caatggaaga gatcttatgg gtaaaagaaa aactggcaga agccaaacag
    15121 caactacgta acgaacacat tccgtttgat gagaagatcc agctcggcat tatgctggaa
    15181 gtgccgtcgg tgatgttcat catcgatcaa tgctgcgaag agattgattt ctttagtatc
    15241 ggtagtaatg acctgacgca atatctgctg gcagtggatc gcgataacgc taaggttact
    15301 cgtcactata acagcctgaa tccggccttc ttgcgggcgc tcgattacgc cgtgcaggcg
    15361 gtgcatcgcc agggcaaatg gattggtctg tgcggtgaac tgggagcgaa aggttccgtg
    15421 ctgcctttgc tggtcggttt agggctggat gaactcagca tgagcgcacc atcaattccg
    15481 gcggcgaaag cgcggatggc gcaacttgat agccgtgagt gccgccagtt gctcaaccag
    15541 gcaatggcct gccgtacgtc gctggaagtg gaacacctgc tggcgcaatt ccgcatgacc
    15601 caacaagacg caccgctggt caccgccgag tgcatcacac tggaaagcga ctggcgcagc
    15661 aaagaagaag tgctcaaagg catgaccgat aacctgctgc tggcgggccg ctgccgctat
    15721 ccgcgtaaac tggaagccga cttgtgggcg cgcgaggccg ttttctctac cggtctgggc
    15781 tttagtttcg ccattcccca cagcaaatca gaacacattg agcaatccac catcagcgtg
    15841 gcgcgtctgg cagcgccggt gcgctggggc gatgatgaag cgcaattcat cattatgtta
    15901 accctgaaca aacatgctgc gggcgatcag cacatgcgca ttttctcgcg cctcgctcgt
    15961 cgcatcatgc acgaagaatt ccgtaacgcg ctggttaacg ccgcctctgc cgacgctatc
    16021 gccagcctgc tgcaacatga actggaactg taaaaggaaa catcatggaa ctgtatctgg
    16081 acaccgctaa cgtcgcagaa gtcgaacgtc tggcacgcat attcccgatt gccggggtga
    16141 caactaaccc gagcattatc gctgccagca aggagtccat ctgggaagtg ctgccgcgtc
    16201 tgcaaaaagc gattggtgat gagggtattt tgtttgctca gaccatgagt cgtgacgcgc
    16261 agggtatggt ggaagaagcg aagcgcctgc gcgacgccat tccgggcatt gtggtgaaaa
    16321 tcccggtaac ttccgaaggt cttgccgcca taaaaatgct gaaaaaagag ggtattacta
    16381 cacttggcac cgctgtatat agcgccgcac aagggttatt agccgcgctg gcaggggcaa
    16441 aatatgttgc tccgtatgtt aaccgcgtcg acgcccaggg cggtgacggc attcgtacgg
    16501 ttcaggagct gcaagcgctg ttggaaatgc acgcgccaga aagcatggtg ctggcagcca
    16561 gctttaaaac gccgcgtcag gcgctggact gtttactggc aggatgtgaa tccatcaccc
    16621 tgcccttaga tgtagcgcaa caaatgctca acacccctgc ggtagagtca gctatagaga
    16681 agttcgaaca cgactggaat gccgcatttg gcactactca tctctaaagg agcaattatg
    16741 gaccgcatta ttcaatcacc gggtaaatac atccagggcg ctgatgtgat taatcgtctg
    16801 ggcgaatacc tgaagccact ggcggaacgc tggttagtgg tgggtgacaa atttgtttta
    16861 ggttttgctc aatccactgt cgagaaaagc tttaaagatg ctggactggt agtagaaatt
    16921 gcgccgtttg gcggtgaatg ttcgcaaaat gagatcgacc gtctgcgtgg catcgcggag
    16981 actgcgcagt gtggcgcaat tctcggtatc ggtggcggaa aaaccctcga tactgccaaa
    17041 gcactggcac atttcatggg tgttccggta gcgatcgcac cgactatcgc ctctaccgac
    17101 gcaccgtgca gtgcgttgtc tgttatctac accgatgagg gtgagtttga ccgctatctg
    17161 atgttgccaa ataacccgaa tatggtcatt gtcgacacca aaatcgtcgc tggcgcacct
    17221 gcacgtctgt tagcggcggg tatcggcgat gcgctggcaa cctggtttga agcacgcgcc
    17281 tgttcccgta gcggcgctac caccatggcg ggcggcaagt gcactcaggc ggcgctggca
    17341 ctggcagaac tgtgctacaa cactctgctg gaagaaggcg aaaaagcgat gcttgccgcc
    17401 gaacagcatg tagtgactcc ggcgctggaa cgcgtgattg aagcgaacac ctatttgagc
    17461 ggtgttggtt ttgaaagcgg cgggctggcg gcggcacacg cggtacataa cggcctgacc
    17521 gctatcccgg acgcgcatca ctattatcat ggtgaaaaag tggcatttgg tacgctgacg
    17581 cagctggtac tggaaaatgc gccggtggag gaaatcgaaa ccgtagctgc ccttagccat
    17641 gcggtaggtt tgccaataac gctcgctcaa ctggatatta aagaagatgt cccggcgaaa
    17701 atgcgaattg tggcagaagc ggcatgtgca gaaggtgaaa ccatccacaa catgcctggc
    17761 ggcgcaacgc cagatcaggt ttacgccgca ctgctggtag ctgaccagta tggacaacgt
    17821 ttcctgcaag agtgggaata acctactcca aactcccggc ttgtccggga gtttgaacgc
    17881 aaaattgcct gatgcgctac gcttatcagg cctacgcaat ctctgcaata tattgaattt
    17941 gcgtgctttt gtaggccgga taaggcgttc acgccgcatc cggcactctg tgccagtaat
    18001 ctgaaactcc cacgttgttc aggaattttt tatccgcttc tggacatttt ctctacagaa
    18061 caatcgttag cccggaaggt cgaatctgtg actcttattt tcacggacga accagatgaa
    18121 agcttcactg gtgctggtca gcctgctaac ggcaattacc agccactcct taaaatctcc
    18181 cgccgttcca ccaaccgtgg tacagatcca ggccaacacc aaccttgcca tcgcagacgg
    18241 tgccagacag caaatcggca gcacgctatt ttacgatccg gcgtatgtgc agcttaccta
    18301 tcctggcggt gatgtcccgc aagaacgcgg cgtatgttcc gatgtggtga tccgtgcatt
    18361 gcgcagccag aaggtcgatt tgcagaaact ggtgcacgaa gatatggcga agaattttgc
    18421 tgcgtacccg caaaaatggc agttaaagcg cccggacagc aacatcgatc accgtcgggt
    18481 gcctaatctg gaaacctggt ttacccgcca cgataaaaca cgccccacca gcaagaaccc
    18541 cagtgactat caagcgggcg atattgtctc ctggcgatta gataacgggc tggcacatat
    18601 tggggtggtg tcagatggct ttgcccgcga cggtacgccg ctggtgatac ataacattgg
    18661 cgcaggcacg caggaagaag atgtactgtt tagctggcgg atggtggggc actatcggta
    18721 ttttgctaaa tgagcgctcg ccccacatgt aaaaaattca gatccttttt acgctgacgg
    18781 cttctttttt cttacggctc accagcgcca gtgccagcac aatcagcaca atgccgctac
    18841 cttcaacggc ccccggattt tcaccgagca gccaccagga aaagagtacg ccgcaaacgg
    18901 gaacggccag tgtgcttaag ctggcaatac tggcaggcaa gtttttcaat acaaacaacc
    18961 ataagctcca cgccagcgct gtcgccagaa tcgcactgta ggccagcgcc cagaacacgg
    19021 tgggttgcca gtcaatttca cgttgcggca ccagtaaagc gaccacgctc atcaccagcg
    19081 ccgcatacag catctgccag gatgttagcg acaataaatc cacgcgcgga tggcgggcat
    19141 acagacgttt agcaacaatc gcgctcgccc cccaactcac accggagagg attgccagca
    19201 tggcgctttt catcgaagag aaatcgagtt gccacggctg caacaccaaa aataagccga
    19261 aagcggcaat cagaatcgcg aaatattgtc cgcgtcgcag gcgttcaccg agaaacaacg
    19321 cggcgaaaat taccacccag aacggcatgg tatagctcag gattgccacc ttccccgctc
    19381 ctccgctgac cagcgcccac tgcgccaggc cgaccatccc gcaggtttgt aacagggcaa
    19441 tggctaaggt gtatttaaac ggcgtcgggc gcatgccgcg accgcgtaat aaaaggacga
    19501 tgaataaaac gagagcgccg aaaatgcagc gtaaggcggt aaagtcgaag gcaccgatgt
    19561 agctggtgac ttgcttcatg aaaatccagc tataactcca gataagtgtg agcacaacca
    19621 ggccactgat tgccagtggg ttgctctttc ctgcggcaga catgagagat ccagtgtgta
    19681 gttttcctgt ggacactata cgccggtaaa gaaacttcag cgactgcctg aaaggcagtc
    19741 gctgaagaag gtcagattac agcaggtcga aacggtcgag gttcatcact ttcacccatg
    19801 ccgccacgaa gtctttaaca aacttctcgt gggcatcgct gctggcgtaa acttccgcca
    19861 ccgcacgcag gacagagtta gaaccaaaga ccagatccgc acggctggct gtgtatttca
    19921 cttcgccggt ttcgcggtca cggccttcga acagctcttt cgattcgtcg gtcgctttcc
    19981 actcgtaacg catatccagc aagttcacga agaagtcatt gctcaatacg ccaacgcggt
    20041 cagtgaagac gccgtttttg ctgccatcga agttggcacc cagtacgcgc atcccgccca
    20101 ccagcgcagt catttccggc gcggtcaggg tcagttgctg ggctttatca atcagcagtg
    20161 actcggtggt ggaaacgtcc agacgagcgc gatagttacg gaaaccgtca gcaattggct
    20221 ccagcagttc gaacatctca atgtcagtct gatcctgacg cgcatcaacg cgacccggcg
    20281 caaacggtac atgaatgctc aaacctgcgg cgcttgcggc tttctcaaca ccaaccacac
    20341 cagccagcac gatgatatct gccagcgagg ctttaccaga ctctttctga attttctcca
    20401 gaactggcag agcacgaacg gctgcggcgt tcacatccca gtcgcgctgc ggcattaatg
    20461 ccagacgtgc accattggca ccaccacgtt tgtcgccacc acggaaggta gaagcagacg
    20521 cccaggccac cgataccagc tcgctgacag acagaccaga atccgcaatc gcgaatttca
    20581 gatcgataat gtcctgctcg gtcgggttgt agatcggctg cggcagagga tcttgccaga
    20641 tcagatcttc tttcggtact tccggcccga tgtagcgaga tttcggcccc atatccctgt
    20701 gcgtcagttt gaaccaggca cgggcaaagg cttcgttgaa cgcctgcgga tcgttgagga
    20761 aacgacgaga aatcttctcg aactcaggat caaaacgcag cgtcaggtcg gtcaccagca
    20821 ttgtcggttt acgtttcttc gacggatcga acggatccgg gataatttcc ggtgcgtcta
    20881 ccgcttcaaa ctggattgcg ccagccgggc tgcgggtctg tacccactca tatttgaaca
    20941 ggttctcgaa gaaatagttg ctccactggg tcggtgtctg ggtccagacc acttccagac
    21001 cagaggtgat ggcatctgcg ccaacgccgc tgccgtaagt gctcgcccag cctaaacctt
    21061 gttcttcaat cggtgcagct tctggatcag gacctacatt tgatgtcgga ccggcaccgt
    21121 gagttttacc cagcgtatga ccacccgcaa tcagcgccac ggtttcttcg tcgttcatgc
    21181 ccatgttgcc gaaggtcgcg cggatagctg ctgccgcaga aagcggttcg ccgctgtgat
    21241 ccgggccttc cgggttaacg taaatcaggc ccatctcggt tgcgcccagc ggtgctttcg
    21301 ccagtgcttc cggatgacgg tgggtcagcc aggctttttc atcaccccag ttaacatcca
    21361 gatccggttc ccagacgtct tcacgaccgg caccaaaacc gaaggtacgg aagccggagt
    21421 tttccagcgc cacgttaccc gcgaggataa acaggtcggc ccaggagatt ttctgaccat
    21481 atttctgttt gattggccac agcaggcgac gcgctttatc gaggcttacg ttatccggcc
    21541 aggagttcag cggtgcaaaa cgttgctgac cacgacccgc gccgccgcgt ccgtcgatgg
    21601 aacggtaagt acccgcgccg tgccaggcca tacgaataaa cagaccggca tagctgcccc
    21661 agtcggcagg ccaccacggc tgagattctg tcagcagggc tttcagatct tttttcaggc
    21721 cgtagtaatc taatttgctg aattctttgc ggtagtcaaa gtcctcaccc agcgggttag
    21781 aacgattaga atgttggttt aacaggtcaa cacggagttg atttggccac cagtcgcgcg
    21841 tggttgtgcc cccacccgcg ctctggtcgt gaccgccctg atggaacggg catttgccag
    21901 tggctgtcgt gttatggata tcgtctgacg tgctcatcaa tgtgctcccc tctacagtgt
    21961 taccgttacg atacacagcg ttagagagaa gttataattg aattaatcca cggatgcgat
    22021 agttgagatc ttacatattg ttggttaaag agatgtagat caaattgatc gtaattaagc
    22081 ttttttgatt atgtccgaat tcggacaaat gctttataaa aaaggatttt agcttcatat
    22141 ccttcaagta atgagggaga aaacaaggct gacaacctta ttttcccgcc ctcatttcga
    22201 ggcagcattt tgtgctctgt ttaaaatttg tgatcactgt gtgattttca caaaagccac
    22261 actatttata aaccaggtcg aacacccagc gtatggcaaa tcgcgtaact catttcagca
    22321 cggttaagcg tatagaagtg gaaatctttt accccttcac gacttaaaat cttcaccata
    22381 tccatggcaa tattcgcgcc caccagcttg cgggtttcag cgtcgtcgtc cagcccgtca
    22441 aacatttgcg ccatccacgc cggaatacgc acgttggtca tgtcagcgaa tttcttcgcc
    22501 tgtttaaagt tagataccgg caaaatgccc ggaatgattt ccacatcaat gcctgccgat
    22561 acgcagcggt cacgaaaacg caggtagctt tcgacatcaa agaagaactg agtaatcgcg
    22621 cggttggctc cggcatcgac tttgcgtttc aggttgagca aatccgcctg agcgcttttt
    22681 gcttccgggt gaacctccgg atacgccgcc acggagatat cgaaatctgc cacttctttt
    22741 aacagcgtca ccaggtcaga agcatacatt tccggcttac cgcttcccgg cggcagatcg
    22801 ccacgcagcg ccacgatatg acgaataccg ttattccagt agtcacgtgc aatggtgcgc
    22861 agctcgtcgg gcgtcgcatc aatgcaagta agatgcggtg ccgcttccag accagtgcga
    22921 tctttaatgc ctttaataat gctgtgcgta cggtcacgct cgccggagtt cgcgccatag
    22981 gtcaccgaca caaacttcgg tttcaggttg ctaaggcgat cgatggagtt ccacagggtc
    23041 tgctccattt cactggtacg cggcgggaaa aactcgaacg aaacgttaat ctgcccctgg
    23101 acttctgcca gactctgatt cagggcatcc cgctggctgg cgtgaaaaaa gctcatacct
    23161 tacctcatca atcgcttgta gttatatgtt gtgttgtgaa catctatcca tttagacgtc
    23221 cagatgtaaa gatgaaggaa aagccgaggg gcgtcaaccg aaaaatgacc aatgatggtg
    23281 aggaaaaatc agggaagttg aaaaaaactc atcttaatta gaggcgataa aagatttctc
    23341 tatagcgcaa tccacctcgc acgttttgac tgatattctg acaacacagc gacacctggg
    23401 cttattcgaa aaacaacgtc ctgccaccgg atggtgatgc taaggcatct gattcggcct
    23461 gcgccctcgc gtaggccgaa tactggatct gcaaaatcaa ctggtaacaa acagatgaat
    23521 caactcaata cgtactttta cttgatatct ttcacgcgca taccgttgat ctgttcatcc
    23581 gtgatggtgt tacccgcttt gaagtaatca accacggcgt gataaacgta ataaccgccc
    23641 gttgtgttac gcgctttccc ttcggtaaag gcggtaaaac catctccacc atcagcaagg
    23701 aaactctgag tagcaatgtg gtaaatcgtc gcatcttcaa tgggtttgcc attcagagtc
    23761 agcgtgatta cccgctgacc aaccggctta ctgctgtcgt acttcatttc caggcctttc
    23821 gatacctgta aaacgccatt actcaaactt gcgccgtgtt ccatcaaact gcgtaattgt
    23881 ttacccgtga gatccatcgt caccagttcg ttggggaacg ggaaggtact gatgacgcca
    23941 cccatcgtaa ttgcacccgc cgggatctca ttgcgaatcc cgccagagtt ggttaacgcc
    24001 aactgggtat ttttacccgc cgctgccagc aaagcgtctg ccgccaggtt cccgagagaa
    24061 gctgattcac cataggcacg ttttagttca accggcgatt gcgccaccgt ttgttgcacg
    24121 acttcatcca gctttttgtt ccaactatca atcacctgtt tcgtttgcgg atcgggcttc
    24181 cactcatcgg cgtaaagggt tttaagctcg aagtttttca ccgtaaaagt gtgcggcttc
    24241 tctttgtagt cgagaaccag tttaccgaca tcaatcccgc cgctgtcagt ggagaggatt
    24301 aacgtattgc cgactttaat cggttccggc gtacccacat gtgcatgccc ggtgatcaaa
    24361 atatccaacc ctttcacctg acttgccgtc tgaatatctt tatccagtgc gcgacgcaca
    24421 tccgtgcctc ccatactgga ctggcgggcc ggaacacctt cgtggatcag ggcgacggtt
    24481 agatcaacct tgcctttgag ttcatcgata taacgttgta gccatttaat ttcatcacgc
    24541 gcctcaatac ccactcgcgt tgccgcagat accgtatcat taaaggcaaa tacaccgtgc
    24601 aaaccaatca cgccaatttt cacgccgtct ttttcaatga tggtataggg cttatcccag
    24661 aatgatttac tgctgttctg ataaaaaaca ttaccctgca caatcgggaa ttttgcctga
    24721 ctcaactgta ataacgtatt gtcccagccg tgatcaaatt catgattacc tatagtgacc
    24781 gcatcgaatg gcatggtatt cataatatcg ataatcgcct tgcctttagt caggctgctg
    24841 atatacggtc cggtaaaata gtcacccgca tcaaaaaacc aggtcgcttt gtttttagct
    24901 ttttcttgtt taacaagcgt agtgatattt gcccagccgc caatatcccg tttaccgtca
    24961 gcaatccacg gcactttata aggctctaca tgtgcatgga ggtcgttggt ataaatgatg
    25021 gtgacatctt tggcgcaagc ccaaaacggc agtgttaatg cgatacccgc agcaagcatt
    25081 ttaattttca taaacaatcc cttccagtga gataaaggat tgctacatta cgttctgaaa
    25141 ggctttttta tgtgagaata ctcacatcca gaggcgataa ctgtaggaca aaatcaacga
    25201 ccttatgaaa tcgttggtga tatatgtcaa atctgtttgc gattaatgtc tgtaaaacgt
    25261 tcggttgtcg aaatttggga ttggcctcat cggaagatta cagttggcca gactataaac
    25321 tgggttttcc ggcactgcac tgccaggcat gcggaagtta cccccctttg tttgatgaac
    25381 aacaatttcg tgactggttg tcagttcaca tgactgcgtg tgccatagaa acagggcatt
    25441 tttgtccatc atgctattgt aaagaaaaga ttttttatgg ccacaacccg cagggaacac
    25501 aacgcgttca atgccgttca tgcaaaaaag tctggactcc gaaacagcag ccagcaagag
    25561 aaattacgta tccccagtcc attgaaaccg ttcaactgtt catgcccttt caaggagcca
    25621 gcgcagtaca aaagctctat gttttagtca gtcttgacgc cactcgtggc aacatccttc
    25681 atctctccac gaattatacg caacatcaga caggagacag tctgcggtac agttacagag
    25741 gcaatacaga accaactgag caccatagcg atattgtgca gagggtagat atgcgtgaag
    25801 cgcaattctt gcgccggagc cagttcgatg aaattcagta tggcagtgct gtgctcaagc
    25861 gcaacggcaa gggagccata ctacgcccgg ttatcacggc acacgggcat ttcagaatac
    25921 tgaaaatccg ctttccacat gtcaaaacgc atatcatttc acacgaatgt tttctgagag
    25981 gcgcaattat tacagcctgg gcagatcagt tccgccaaca acaaggcgaa ctttggttcg
    26041 tagaagaaga aatcagcgac cgtaacgctg atattccctg gcattttcag ggaacgacat
    26101 accatggttg gtggcaaaat cagtggcaac gctgggggca ggggaataac agcaagatgg
    26161 tctgcctgct cacaggagtc tccttagaaa ggggcgcaaa tgtttctctg gcaaccagtc
    26221 gttgctttat cacatggctg acagaccaac acgactttac ccaaagcgcg ttattatccg
    26281 caggtcgcgt aacgaaaatg ctgacctcac tggcgttaaa atacaatgaa tcgctcactc
    26341 catcttgtta gtcaatacac tcgacaaacg accataaatt aatgggatca ctatttctca
    26401 tttaattaaa tatatgaata tatgactgaa aaatataatc acagtcaatg tttaattaat
    26461 aacattattt gttaccaaga tcacataata ctttcctgac ctatgcttac ataaaaataa
    26521 gacacggtgt tccttttatt taaataattc aaccaatgta acaaccccta tcaaggaggg
    26581 atgatgagaa ctgtatttac gactttatca gtcatattca gcgtgatgtt ttctcacagt
    26641 atactagctc aggacgttac tatttattat acgaatgata ttcacgcaca tgtcaatccg
    26701 gcaaaaattc ccgctgttga taagaacaga cttgtaggtg gtatggctaa tattgcaggc
    26761 atcgttaatg aagcgaagaa aaaaaacaaa gatgtctttt tctttgatgc aggcgactat
    26821 tttaccggtc cgtatatcag taccctgacc aaaggtgaag ctattattga tatcatgaac
    26881 accatgccct ttgatgcggt gtccgttggt aatcacgagt tcgatcacgg cgtgcccaat
    26941 atggtatctc agttatcaaa agcaaaattc cccattctgc tgggaaatat ttactacacc
    27001 gatacaaata aaccagtttg ggatcatccg tggaccatta tcgaaaaaga tggtttaaag
    27061 attggtgtga ttggattgca tggtgcattc gctttctatg atacagtcgc ggcaaaagca
    27121 cgtgaaggcg tagaagccag agacgaaatt aaatatttaa ataaagcact tgcagaatta
    27181 aaaggtaaag tcgacattac cgttttgcta attcacgaag gtgttccggc gcgtcaatca
    27241 agctttggta gtaaagatgt ggagcggcta ctacaggcag atattgagac agctaaaaaa
    27301 gtgaatggtg ttgatgtctt aattaccggt cacgcacatg tcggaacacc gcaaccaata
    27361 aaagttaata acacattaat tgtatctacc gatgcatacg gcaccgacat cggaaaactt
    27421 gtgcttgatt acaatccgaa aacaaagaaa attgatagtt ataatggtga gttaatcacc
    27481 atctttgcag atcaatttaa acctgacacc atcgttcaaa atactatcga taaatggagc
    27541 gtaaagctta acaaaataac acaggaagtt gtcggccatt ctcccgtagt tttaacacgt
    27601 gagtatggca gttcttcttc taccggtaat cttattctgg atgcaatgat ggaaaaaaca
    27661 cctgatgcca ttgccggatt tcaaaatagc ggtgggatgc gagctgattt tcctaaaggt
    27721 gatatcacac tgggagatgt tattagcaca ttccccttta ataatgacct catcgagatg
    27781 gatttgacgg gccgcgatct caaatcgttg atgacgcatg caaccaatct aactaacggt
    27841 gtgttacagg tttcaaaaag cgttgcggtt gtctatgaca gcaaaaaacc actcaaccaa
    27901 cggttaatct ctttcaccat taacggcaaa cccgtggaag ataatcaaac atatcgtatt
    27961 gccacgcact ccttttgtgc cagtggtggt gacggttttg aagcattttt gaatggaaaa
    28021 aatgtgaaga cgataccggg aacaacctcg gcggaatcta tcatcgatta tttcaaaaat
    28081 cataaacctg tcaccccaga cttaactaaa cgagtcatgg acgtcgccaa ataaaatatc
    28141 atttatcagt ctatatattt aacaaggaat gcgaatgaaa acagtgcagc gtactttact
    28201 tgccgcaaca ttgctgacta ttttttcggt ctcagtcatg gcgcaggatg tcacgattat
    28261 ttatacaaat gatcttcacg ctcatgtgga ttcttataaa gtgccgtata tcgcagacgg
    28321 aaaacgcgac attggcggct ttgccaatat atctacttta gtaaaacagg agaaagcgaa
    28381 aaataaagcc accttttatt ttgatgctgg agactatttc accggccctt atatcagcag
    28441 tctgacaaag ggcgaagcaa taattgacat tatgaatacc atgccttttg acgcagtatc
    28501 aatcggtaat catgagtttg accatggctg ggataatgcc ctgcgacaat taagcaaagc
    28561 gaattttcct gttttattgg gcaatgttta tcataaagag agtgagaacc ccttctggaa
    28621 caagccctac accatcctgg aaaaagatgg catcaagatt ggtattattg ggctacacgg
    28681 cgtatttgca tttaatgata cggtctcgga gctctctctc cagggactcg ataatgacaa
    28741 taataaccgc tttgataaat cttccgagac ccttaagaat caggggattg aagcacgtga
    28801 tgaagtgaag tatttgcagc attatatcga cgagttacgt gataaggttg acctcactgt
    28861 ggcgctggtt catgaaggcg ttccggcgcg tcagtccagc attggcaata cagatgttag
    28921 acgcgctctt gataaagata ttcaaaccgc tagcaaggtg aaaggattgg atattctgat
    28981 taccgggcat gcccacgtcg gaacgccaga accaatcaaa gtcggcaaca ccttaatcct
    29041 ttcaactgat agtggaggta tcgatattgg taaattagtc cttgatgtca accctacagc
    29101 tcgtacccat aaaatgaaga gctttgagct gaaaacagtt tatgccgatg aatggatacc
    29161 cgatccaacg acacaaaaag ttatcaatgg ctggaataaa aaactggcgg atattgtacg
    29221 ccaaccagtg ggtgaatcat cgattgcatt aactcgcgcc tatggtgagt catcgcaact
    29281 aggcaacctg tttaccgatg caatgcttgt cgctgctccg actgcacaga ttgcattgat
    29341 aaactccggc agcttacgtg cggatataaa tgcaggcacg attacctttg gcgacattac
    29401 cagtacattc cccttcaaga atgaacttac tgaaatggat ctcagcggta aggatctgcg
    29461 taacctactg gaacacgggg catcgcttac caatggcatc ctgcaaatgt caaaaggcgc
    29521 agaaatgcgc tataccccgc agaaaccagt aggtcagcgc atagtatctt ttaaaattaa
    29581 tggtgaagag atcgttgata cgaatattta ccatgttgca acaacgactt tccttgcact
    29641 ggggggcgat ggttttctgg cattcaaaga agggaaaaat gttcaggtcc gcgccggaaa
    29701 taacatgtcc gatgtcgtga tcgattattt gaagaaaggc cacaagatta cgcctgcaca
    29761 ggtaaatgaa atgcgggtgg acgttagcaa ataataaaaa aattataatg ccattcattc
    29821 actgaatggc attattttct ctaaatcaac aatagataat gagatatatt tatgtgtata
    29881 aaaaagcacg ggatagcttt atcgattcta ttaatacctt gcattggaaa cgctcaggaa
    29941 attaaaagtg aggaaggctg gttacatcaa agcttgaatg tcattggtcg cacagactct
    30001 cgctttggcc caaggctgac taacgacctc taccctgaat atactgtagc aggaagaaaa
    30061 gactggtttg atttttatgg ttatgttgat ctaccaaaat tctttggcgt cggcagtcac
    30121 tatgatgttg ggatctggga tgagggctca ccactattta cggaaataga acctcggttt
    30181 tccattgaca aattgaccgg attaaatctt gcgttcggcc cttttaaaga atggttcatt
    30241 gcaaacaact atgtctatga tatgggtgac aaccagtcat cccggcaaag tacatggtat
    30301 atggggcttg gtacagatat cgacacgggt ctaccaatta agctttctgc caatatatac
    30361 gccaagtatc agtggcaaaa ctatggtgcc gctaatgaaa atgaatggga cggatatcga
    30421 ttcaaaataa aatatagcat tcctcttacc catttattcg gagggcgatt ggtatacaat
    30481 agttttacta actttgattt tggctccgat cttgcggaca aatcacacaa taataaacga
    30541 accagtaatg ctattgcttc aagccatatc ctttccctgc tatatgaaca ctggaaattt
    30601 gcatttacac tacgttattt tcacaacggt ggacaatgga atgctgggga gaaagttaac
    30661 ttcggagatg gtccatttga attaaaaaac acaggatggg gaacctatac tactattggt
    30721 tatcaatttt aaatcaggtg atgttttaga tatttttact taatgccatt ttgtaattgt
    30781 cataaataac ttcaccattc acactgtgaa ttcatcgctc aacacaaaag cccccttatt
    30841 accggggcac tgaccaaagg caaaatgaca atcaatcacc acctccgcag gaggtggttt
    30901 agaggtgaca acaaaagctg gcaactgcca gcttttgttc aataatcact acaacaactg
    30961 cgccagacgg ttaatatccg actgaatcgc cccggcggtg acatcgcgcc ctgcacctgg
    31021 cccgcggatc accagcgggt tgtcgcgata ccagcggctt tcgatggcaa agacgttatc
    31081 gcacggtagt agtgatgcca gcggatggtc ttctcgcacc gcttccacgc ctactcgcgc
    31141 tttgccgttg gcatcgaaac gcgccacgta gcgcaacacc agccccattt cacgggccgc
    31201 ttccaaccgt tgtaccatct gctcattaag ttcatcgcca ttttcaaaga aatggtcgat
    31261 gcttccacct tcgcaatggg ttggcaccag tgattcaaca cgcacctggt ccggttcaat
    31321 gttgtaaccc gcttcacgcg ccagaatcac cagcttgcgc atcacgtctt tgccggaaag
    31381 gtcatcacgc ggatcaggct cggttaagcc ctgctgccac gcctgatcca ccagctcggt
    31441 aaacggcacg ctaccgtcga attgcaggaa cagccaggag agcgtgccag agaagatccc
    31501 actgatcgac aaaatggtat cgccgctgtc gatcagatcg cgcacggtat ggttgatcgg
    31561 caaacccgca ccgacagtgg cattgtacag ccagtgacgc ccggtttttt cgaaggcgtc
    31621 gtggatctgg cgatatttat tgctgtcgct ggctcccgcc agtttgttgg cgctgatgac
    31681 gtggaaaccg tggctggcaa aatcgagata ctgatcagca agctgctggc tggcggtaac
    31741 gtccagcacc actaaatcat catacggatg ggcgcgcatc cacaggaaca gcgactcttc
    31801 atcctgctcg accgcttcat cgttgaagaa ggctaacgcg cggctggcgt ccagcccgtc
    31861 atagctcaac aggctgcggc ggctgtccac cacgcctgcc agcacaaatt caaagccggt
    31921 acgcgccgaa agcgtgctct gctcacgggc gaacagttcc agccagcggg aaccgatatt
    31981 gcccttgccg aacaatacca ggccgatgcg tttttccgcg cgaaagacgg actgatgcag
    32041 accctgaatc aggctttcgg tcggcccggt gcgcagtact gccaccaggc tgatgccgtc
    32101 atcggactgc caggtaaatt cgaccggctg gcctttcagt tgctgccaga agcggtggca
    32161 atgcagcggg ttacgggtta cgcccgcccc aaccatcgcc accagcgcca gcccctgacg
    32221 caggcgcagt tcgccaggta atcccgcttc gtcgagaatt ttcagcgcac tgtcagcgac
    32281 ttctgaggtg tagcaaaatt gcagcaactg gcgatcgtta tgtacgccaa ccgccagtgg
    32341 gcgcacctga gcacgtttca ggatctggtc gatctcttta tgcgccagtt tgaaatcctg
    32401 actggcgggc acctgaaact caatcaaaca gacatcatcg tggctggtga caatacgcgc
    32461 gccagtacct gaggccagca cgcgttcaat gcgcgtggaa ccttgatccg gcgtgtagct
    32521 acagcgcagt tgcaggtcga tttcactacc ggatactggc tgtaaagtac gggcgtgaag
    32581 aacgggggcc gccagacgcg ccagttcact ggcttcatca agacgcagca atggcagcag
    32641 acaggcatct ttcactttac gcgggtcggc gctgtatacc ccggcgacgt cgctccagat
    32701 ggttacgcga gaaacacccg ccagcgcacc gatttgtgtc gcggaatagt cggaaccgtt
    32761 acgccccagc agcaccgttt caccggcgtt gttacggctg ataaacccgg tcaccaccag
    32821 acgtttgccc ggatgttgta ccagtagctg ttgcagcaac gggtaagaaa gcccttcatc
    32881 aacctgcggc tgcgcggcgc gttcagcgcg taaaaactcg cgggcatcaa gccaggccgc
    32941 tggcagccct tgttgattaa gtaccgcaga catcaggcgc gccgaccaca cttcaccgtg
    33001 gcccaccact tctgcataca ccgcatcgtt aataccgctg tcgagcagcg ccgccaggcg
    33061 ttccagatcg ctgacaaaag cgctaatgag gctatcggct tcttcagcgg gtagcaggcc
    33121 gctaatcaga tcgcactgat aacgacgtag cgtttgttga acctgatgcg cagagagacg
    33181 atcggtctgg cttagtttca accagttaat caactggtta gtggtgctac cggcggcgga
    33241 aaccaccatc atatcgtcag gctgagagta ctccgccata atgcccgcga cacgcaaata
    33301 acacttcaca tcagccagac tactgccacc aaatttatgc agttgacgac ctttcgcccc
    33361 tgcctgcgca atcacactca tttttacccc ttgtttgcag cccggaagcc attttccagg
    33421 tcggcaatta aatcttcgcc atcttcaata ccggtggaga tacgcagcag cgtctcggag
    33481 atcccggcgg cagcacgcgc ttctggtgcc atgcctgcat gtgtcatggt tgcggcgtga
    33541 gagattaaac tttccactcc ccctaatgat tccgccagcg taaacaacga cagcccaccc
    33601 aggaaacgac gcagcgtctg ctcatcgcca tccagttcaa aactcaacat tgcgccaaag
    33661 cctttttgct ggcgcgcggc aatttcatgc ccctgatttt ccggcagcga cgggtgatac
    33721 agttttttca ccaacggctg ggtttgtaga tatttcacaa tcgcctgcgc gttgcgctgg
    33781 gccagctcca tacgcggcac cagcgttcgc aaaccacgta gcagcagata gctgtcaaac
    33841 gcgccgcccg tcacgccaat attgtttgcc caccaagcaa gttcagtgac aacgtccggg
    33901 tctttagcaa tcaccacgcc agccaccacg tctgagtgac cgttcagata tttcgtgcat
    33961 gaatgcaaca ccagatcggc acctaatgcc agcggatttt gtaatgccgg gcttaagaag
    34021 gtgttatcca ccacgctcac cgccccggct tcccttgcca gatggcagat tttcgcaata
    34081 tccacgacgc gtaacaatgg attacttggg ctttctacca gtaccagttt ggggttttct
    34141 gccagcgctg cccgtaatgc ctgttcatcg ccttgatcaa caaacaacac gcgatagcaa
    34201 ccgcgttttg ccagactgtc gaacaggcga tagctaccgc cgtagcagtc gtgcggcgca
    34261 accagcagat cgccaggttt caaaaagacg gtcgttacca ggtgaatcgc ggacatgccg
    34321 gtattagtaa gtacggcacc ggcaccgcct tccagttctg ccagcgcacg ctgaaccaca
    34381 tcgcgcgttg ggttgccacg acgcgagtaa tcatgcgcgc gcggttcatt aaatccggta
    34441 aagttatagg tgctggaaag atggatggga gggacaacgc aaccatactg ttcgtcgtca
    34501 tttaacccgc tacgcactgc gatggtggcc tgtttacgcg tcatgtgatg aagttccctg
    34561 ggcttggtcg gtgaaatgtc aggcaccaga gtaaacattg tgttaatgga cgtcaataca
    34621 tctggacatc taaacttctt tgcgtataga ttgagcaaat cccaaatagc cgttaaaatt
    34681 atatgcatta tcacgccgac aggtgcatta cacgatgtca cggtagcgcc tgtacggtaa
    34741 actatgcggg tttacggtca gtacccacat taactatgtg gtctggtctc aatttattga
    34801 cgaagaggat taagtatctc atggctgaat ggagcggcga atatatcagc ccatacgctg
    34861 agcacggcaa gaagagtgaa caagtcaaaa agattacggt ttccattcct cttaaggtgt
    34921 taaaaatcct caccgatgaa cgcacgcgtc gtcaggtgaa caacctgcgt cacgcgacca
    34981 acagcgagct gctgtgcgaa gcgtttctgc atgcctttac cgggcaacct ttgccggatg
    35041 atgccgacct gcgtaaagag cgcagcgacg aaatcccgga agcggcaaaa gagatcatgc
    35101 gtgagatggg gattaacccg gagacgtggg aatactaaag cggaaaagag cggcgcggag
    35161 ttaaccagcc gcttttttca tacatggacg tttaacgatg aaaatcaggc tactgatcct
    35221 cagcctgctg gtttctgtgc ctgcctttgc ctggcagccg caaactggcg acatcatctt
    35281 tcaaatctct cgctcatcgc aaagtaaagc gatccaactg gcgacccatt ccgattacag
    35341 ccacaccggt atgctggtga tgcgtaacaa aaagccctac gtttttgaag cggtcggccc
    35401 ggtgaaatac accccgctca agcagtggat cgcccacggt gaagatggca aatacattgt
    35461 tcgccgcgtt gaaggtgggc tgaataccga acaacagcaa aaactggcgc aaacggcaaa
    35521 acgttatctc ggcaaaccgt atgactttag tttctcgtgg agcgacgatc gccagtactg
    35581 ctcggaagtc gtttggaagg tttatcagaa cgcgctggga atgcgcgtgg gcgagcagca
    35641 gaagttgaaa gagttcgatc tcagcaaccc gctggtccag gcaaagctca aagaacgtta
    35701 cggcaagaat attccgcttg aggaaacggt agtttcacca caagccgttt tcgatgcgcc
    35761 acaactcact acggtcgcca aagaatggcc gctgttttcg tggtaatacg accaaaattc
    35821 aggcacaaaa aaagcgccgt gcggcgcttt tttcggaaat ccggtcttat ttgctgcccg
    35881 ggatgttgaa acgcttgttg aagcggtcaa cacggccacc ggtagcaaca tcacgctgtt
    35941 tgccagtgaa gaacgggtgg cacttgctgc acacgtcgag gttcaggtca tgaccaacgg
    36001 tggagcggat tttcattacg ttaccgcaag agcagctagc agtaatttct tcgtatttcg
    36061 ggtgaatatc ttttttcatg ggaaaacctc ggtttaaggc cgcgtcgctc ttccagccct
    36121 aacgccagac accacgcgat gttaaaagta tagcttcaat acgatcattt cgtacgaagg
    36181 cgcgaaatca tacagaaatt aaccagcgta tgcaaactga tccgcactct tctacggcaa
    36241 tgtgtatact aacccaccga atttcaagtc aggatgatgc tatgcccgtt gcccacgttg
    36301 ccttgcccgt tccgcttcct cgtacctttg actatctgct gccagaaggc atgacggtta
    36361 aagccgggtg tcgcgtgcgc gtgccgtttg gcaaacagca ggagcgcatc ggggttgtgg
    36421 tgtcagttag cgacgtcagc gagctgccgc tcaacgagct gaaagtggta gtagaagtgc
    36481 tggacgttga gccggtattt actcactccg tctggcgatt actgctatgg gcggcagatt
    36541 actatcatca tccgattggc gatgtgctgt ttcatgcctt gccgatttta ctgcgccagg
    36601 ggcggcctgc ggcgaacgcg ccgatgtggt actggtttgc cactgaacaa ggtcaggcgg
    36661 tggatctgaa cagcctgaaa cgttcgccaa agcaacaaca ggcactggca gcattgcgac
    36721 aagggaaaat ctggcgcgat caggtcgcca cgctcgaatt taatgatgct gcactgcagg
    36781 cgctgcgcaa aaaaggtctg tgtgatttag caagtgaaac accagagttt agcgactggc
    36841 gaaagaatta tgccgtttct ggtgagcggt tgcgattgaa taccgaacag gccaccgccg
    36901 ttggcgcaat tcatagcgcg gcagatacct tctctgcctg gctgctggcg ggcgtcaccg
    36961 gttccggtaa aacagaagtt tatttgagcg tactggaaaa cgtgctcgct cagggcaagc
    37021 aggcgctggt gatggtgccg gaaatcggcc tgacgccgca aactatcgcc cgttttcgtg
    37081 aacgttttaa tgcccccgtg gaagttctgc attccggcct gaacgacagc gagcgccttt
    37141 cggcgtggct gaaagcgaaa aatggcgagg cggcgattgt gattggcacc cgctccgcgc
    37201 tgtttacgcc gtttaaaaat ctcggcgtga ttgtcattga tgaagagcac gacagctcct
    37261 acaagcagca ggaaggctgg cgctatcatg cccgcgacct ggcggtgtat cgtgcgcaca
    37321 gcgagcaaat cccgattatt cttggctccg caacgcccgc gctggaaacg ttatgcaacg
    37381 tccagcagaa aaaataccgc ctgctgcgcc tgacccgtcg ggcgggcaat gcgcgcccgg
    37441 caattcaaca tgtgctggat ttaaaaggtc agaaggtgca ggcaggtctg gctccggcgt
    37501 taatcacccg tatgcgccag catttacagg ccaacaatca ggttatcctc tttcttaacc
    37561 gccggggttt tgcgcctgcg ctactgtgcc acgactgtgg ctggatagcc gaatgcccac
    37621 gttgcgatca ctactacacg ctgcatcagg cgcaacagca tttgcgttgc caccactgtg
    37681 acagtcagcg tccggtgccg cgccagtgcc cttcttgcgg ttccacgcac ctggtccccg
    37741 tggggctggg caccgaacag cttgaacaga cgctcgcacc gttgttccct ggtgtgccca
    37801 tttctcgtat cgaccgcgat accaccagcc gtaaaggtgc gctggaacaa caactggcgg
    37861 aagtacatcg cggtggcgcg aggattttga tcggcacgca aatgctggcg aaaggtcacc
    37921 actttccgga tgtgacgctg gtggcgttac tggacgtaga cggcgcgctg ttttctgccg
    37981 attttcgctc ggcagagcgt ttcgcccagc tttacaccca ggtcgccggt cgtgccgggc
    38041 gtgcgggtaa acagggcgaa gtggtgctgc aaacgcacca tcctgaacat ccgctgttgc
    38101 aaacgttgct ctataaaggc tacgacgcct ttgccgaaca ggcactggct gaacgacgca
    38161 tgatgcagct gccgccgtgg accagccatg tgattgtgcg tgcggaagat cataacaatc
    38221 agcacgcgcc gctgttcctg caacaactgc gtaatctcat cctctccagc ccgctggcag
    38281 acgacaaact gtgggttctc ggtccggttc cggcgctggc tccgaaacgt ggtggtcgct
    38341 ggcgctggca aattttgttg cagcaccctt cccgcgtgcg cttacagcac atcatcaatg
    38401 gtacgctggc acttattaat acaataccgg attcccgcaa ggtgaaatgg gtactggatg
    38461 ttgatccgat tgagggttaa accgctcacg atgcgaggcg gatcgaaaaa ttcaatattc
    38521 atcacacttt tcatgaaaat tctgtaaccg ttttcacgcg ctatctgcta aaaatgttgc
    38581 cgatgtgaag taaacatgga tgtagtacgc ctgacgtgcc aggcgaggag tgagtgtgaa
    38641 agcgaagaag caggaaactg ccgcgaccat gaaagacgtt gccctcaagg caaaagtctc
    38701 tacagcgact gtctcccgag cattaatgaa tcccgataaa gtctcccagg ccacccgtaa
    38761 tcgggttgaa aaagcggccc gggaagtggg ttatttaccg cagcctatgg ggcgcaacgt
    38821 caagcgtaat gaatcccgca ccattctggt gattgtcccg gatatctgcg accctttctt
    38881 tagcgaaatt attcgcggta tcgaagttac ggcggcaaat cacggatatc tggtgctgat
    38941 tggcgactgt gcgcatcaaa atcagcagga aaaaaccttt atcgatttga tcatcaccaa
    39001 gcaaattgat ggcatgttgc tgctgggttc aaggctgccg tttgatgcca gcattgagga
    39061 acagcgtaat ctgccgccga tggtgatggc gaacgaattt gcaccggaac tggagctacc
    39121 taccgttcat atagacaatc tgaccgccgc atttgatgca gtaaattatt tatatgagca
    39181 agggcataaa cggattggct gtatagccgg tcccgaagag atgccgctgt gtcactaccg
    39241 cctgcaaggt tacgttcagg cgctgcgtcg ctgcggcatt atggttgatc cgcaatacat
    39301 cgcccgtggc gacttcacct tcgaagccgg aagcaaagca atgcaacagc tgcttgatct
    39361 tccacaaccg cctactgctg tcttctgcca tagcgatgtg atggcgctcg gcgcactttc
    39421 tcaggcaaaa cgtcaggggc tgaaagtccc ggaagacctt tccataatcg gttttgataa
    39481 catcgacctg acgcaatttt gtgatccgcc gctgacaacc atcgcgcagc cgcgttacga
    39541 aatcggtcgg gaagctatgc tgttattgct tgatcaaatg caggggcagc acgttggcag
    39601 tggctctcgt ttaatggact gcgaacttat catccgggga tcaacacgcg cgttacctta
    39661 aagtaaacct ttaagactgc cgtgactggt caaaggcccg ccgcttaagt aacatggcgg
    39721 gctgacgaac gaataaatac agcgaaacga tagtggcaca acgagattat gtacgccgca
    39781 gccaaccggc accttcgcgg cgaaaaaaga gcacctcacg gaaaaagcaa cgaaatctgc
    39841 ctgcggtttc tcccgctatg gtcgctattg ctgccgccgt tcttgtgacc tttatcggtg
    39901 gtctgtactt cattacgcat cacaagaaag aagagtccga gacgctgcaa agccagaaag
    39961 tgaccggaaa tggactacca ccaaaaccag aagaacgctg gcgctacatt aaagagctgg
    40021 aaagtcgcca gccgggtgtg cgtgcgccca cagaaccttc tgccggtggt gaagtgaaaa
    40081 cgccggagca actgacacca gaacaacgcc agcttcttga acaaatgcag gctgatatgc
    40141 gccagcagcc aacgcagctg gttgaagttc cgtggaatga gcagacgccg gaacagcgtc
    40201 agcaaacgct acagcgccag cgtcaggcgc agcaattagc ggagcagcaa cgcctggctc
    40261 agcagtcacg tacgactgaa caaagctggc agcagcagac gcgtacgtcg caagccgcac
    40321 cggtacaggc gcaaccgcgc cagtctaaac cggcttctac tcagcagcct taccaggatc
    40381 tgctgcaaac ccctgcgcac accaccgcgc agtcgaagcc gcagcaagct gcgccagttg
    40441 ctcgtgccgc tgacgcgcca aaaccgacgg cggagaaaaa agacgaacgc cgctggatgg
    40501 tgcagtgcgg ttcgttcaga ggcgcggaac aggcagagac ggtacgtgct cagctggcgt
    40561 ttgaaggctt tgactcaaaa attaccacca acaatggctg gaatcgtgtg gtcattggcc
    40621 cggtgaaagg caaagagaac gcagacagca ccctcaatcg gttgaagatg gcgggtcata
    40681 caaactgcat tcggctcgcc gccgggggtt gaaaccctca aaatcccccc catctataat
    40741 tgcattatgc cccgtacttt tgtacggggt ttgtactctg tatttgtaac caaggggtca
    40801 gctcgtgaca actatagtaa gcgtacgccg taacggccat gtggtcatcg ctggtgatgg
    40861 tcaggccacg ttgggcaata ccgtaatgaa aggcaacgtg aaaaaggtcc gccgtctgta
    40921 caacgacaaa gtcatcgcgg gctttgcggg cggtactgcg gatgctttta cgctgttcga
    40981 actgtttgaa cgcaaactgg aaatgcatca gggccatctg gtcaaagccg ccgttgagct
    41041 ggcaaaagac tggcgtaccg atcgcatgct gcgcaaactt gaagcactgc tggcagtcgc
    41101 ggatgaaacc gcttcgctta tcatcaccgg taacggtgac gtggtgcagc cagaaaacga
    41161 tcttattgct atcggctccg gcggccctta cgcccaggct gcagcgcgcg cgctgttaga
    41221 aaacactgaa cttagcgccc gtgaaattgc tgaaaaggcg ttggatattg caggcgacat
    41281 ttgcatctat accaaccatt tccacaccat cgaagaatta agctacaaag cgtaaggatc
    41341 tcccatgtct gaaatgaccc cacgcgaaat cgtcagcgaa ctggataagc acatcatcgg
    41401 ccaggacaac gccaagcgtt ctgtggcgat tgctctgcgt aaccgctggc gtcgcatgca
    41461 gctcaacgaa gagctgcgcc atgaagtgac cccgaaaaat atcctgatga tcggcccgac
    41521 cggtgtcggt aaaactgaaa tcgcccgtcg tctggctaag ctggcgaatg cgccgttcat
    41581 caaagttgaa gcgaccaaat tcaccgaagt gggctatgtc ggtaaggaag tggattctat
    41641 tattcgcgat ctgaccgatg ccgccgtgaa aatggtacgc gtccaggcta tcgagaaaaa
    41701 ccgttatcgc gctgaagaac tggcagaaga acgtattctc gacgtgctga tcccacctgc
    41761 taaaaacaac tggggacaga ccgaacagca gcaggaaccg tccgctgctc gtcaggcatt
    41821 ccgcaaaaaa ctgcgtgaag gccagcttga tgacaaagaa atcgagatcg atcttgccgc
    41881 agcaccgatg ggcgttgaaa ttatggctcc tccgggcatg gaagagatga ccagccagct
    41941 gcagtccatg ttccagaacc tgggcggtca gaagcaaaaa gcgcgtaagc tgaaaatcaa
    42001 agacgccatg aagctgctga ttgaagaaga agcggcgaaa ctggtgaacc cggaagagct
    42061 gaagcaagat gctatcgacg ctgttgagca gcacgggatc gtgtttatcg acgaaatcga
    42121 caaaatctgt aagcgcggcg agtcttccgg tccggatgtt tctcgtgaag gcgttcagcg
    42181 tgacctgctg ccgctggtag aaggttgcac tgtttccacc aaacacggga tggtcaaaac
    42241 tgaccacatt ctgtttatcg cttctggcgc gttccagatt gcgaaaccgt ctgacctgat
    42301 cccggaactg caaggtcgtc tgccaatccg cgttgagctg caggcactga ccaccagcga
    42361 cttcgagcgt attctgaccg agccgaatgc ctctatcacc gtgcagtaca aagcattgat
    42421 ggcgactgaa ggcgtaaata tcgagttcac cgactccggt attaagcgga tcgcggaagc
    42481 agcatggcag gtgaacgaat ctaccgaaaa catcggtgct cgtcgtttac atactgttct
    42541 ggagcgttta atggaagaga tttcctacga cgccagcgat ttaagcggtc aaaccatcac
    42601 tattgacgca gattatgtga gcaaacatct ggatgcgttg gtggcagatg aagatctgag
    42661 ccgttttatc ctataatcgc gttcaatcat tttcatcatt gtttgatggg gctgaaaggc
    42721 cccattttta ttggcgcgta ttatgactga acaacaaatt agccgaactc aggcgtggct
    42781 ggaaagttta cgacctaaaa ccctccccct cgcctttgct gcaattatcg tcgggacggc
    42841 gctggcatgg tggcaaggtc acttcgatcc actggtcgcc ctgctggcat tgattaccgc
    42901 cgggctatta cagatccttt ctaacctcgc caatgattac ggcgatgcgg taaaaggcag
    42961 cgataaacct gaccgcattg ggccgctacg cggcatgcaa aaaggggtca ttacccagca
    43021 agagatgaaa cgggcgctca ttattaccgt tgtgctcatc tgtctttccg ggctggcact
    43081 ggttgcagtg gcgtgccata cgctggccga ttttgtcggt ttcctgattc ttggcgggtt
    43141 gtcgatcatt gccgctatca cctacaccgt gggcaatcgt ccttatggtt atatcggttt
    43201 gggtgatatt tccgtactgg ttttctttgg ctggttgagc gtcatgggga gctggtattt
    43261 acaggctcat acattgattc cggcactgat ccttccggca accgcatgcg gcctgctggc
    43321 aacggcagta ctgaacatta ataacctgcg tgatatcaat agcgaccgcg aaaacggcaa
    43381 aaacacgctg gtggtgcgct taggtgcagt aaacgcgcgt cgttatcatg cctgcctgct
    43441 gatgggctct ctggtgtgtc tggcgctgtt taatctcttt tcgctgcata gcctgtgggg
    43501 ctggctgttc ctgctggcgg caccgttgct ggtgaagcaa gctcgttatg tgatgcgcga
    43561 aatggacccg gtggcgatgc gaccaatgct ggaacgcact gtcaaaggag cgttactgac
    43621 taacctgctg tttgttttag ggatattcct aagccagtgg gcagcataac tgacaaatat
    43681 caattaacaa ttgatgattt tgccaacagc ctacatagcg cgatatactg aaaattctcg
    43741 cagcaactga atgttaagcc tatgaaatac gatacttccg agctttgtga catctatcaa
    43801 gaagatgtta acgtcgtgga accgctgttc tccaactttg gcggacgggc gtcgtttggc
    43861 ggacaaataa tcacggtaaa atgtttcgag gacaacgggt tgctgtacga tctgctcgaa
    43921 cagaatggcc gtggtcgtgt tcttgtcgtc gatggcggtg gttctgttcg tcgcgcactg
    43981 gtcgatgctg aactggcgcg tctggcagta caaaatgaat gggaaggtct ggtcatttac
    44041 ggcgcggtgc gtcaggtaga tgacctggaa gagctggata tcggcatcca ggcgatggcg
    44101 gcaattccgg ttggtgccgc tggcgaaggc attggcgaaa gcgatgtccg cgtcaatttt
    44161 ggcggtgtca ccttcttctc cggcgaccat ctttatgccg acaataccgg gattattctt
    44221 tcagaagatc cgctggatat tgaataagta cctgccatgc gttaaaaggg gaattaaatt
    44281 cccctttttt atacatggac gtattcatgc aaaatattat tcttttattt atatatttat
    44341 tacttatagt cgttaaccat ttccgctaca aattcctttt gtctggcaac acgggtgaaa
    44401 tgattttata ttttgctaac gtatttttta acccacttgc cttatcattt attatcgcca
    44461 caatcatctg tataacgata aaaaaaagaa gcagccacag ctttattcgc ggcacttgtt
    44521 ggttaatggg attatttctt atctatagca gctatacagt gtgggaacga cacagcacat
    44581 gggattatac tttcccgggg ccaggtatca ccgtcacagt tccgagcaaa cagtgggttg
    44641 ccaatatagt aaaacaagga ccaatcctgg tgactcggga tgcccatgct attttagtta
    44701 ttgttgcctt ctcacaaaat gaactcggtg tgcattcaat cgaggaactt accgcgactc
    44761 aaaacggaac tgcagaaatg catgtttgtg atgttaaggg atttgcctgc gcttatcagg
    44821 aaggaattca aacaatgagt gatggcaaaa taagacatgc tattttcatg actctactgg
    44881 ataacactaa tttgatacga ctggtcgcag ctatcgaccc agattatttg gacgaatatc
    44941 aagaagaagt aatgaaaatg atactttctg cccatcaata atctgatgtg catgttaata
    45001 ataagggatt gatatgagat acgttatctt attaatacta tggataataa ctacttttct
    45061 tagtcatctt cagcacagca acctactgtc tggagatcct ggcatggttg gcgagtatat
    45121 cggttcagta tttctggggc cgttgttact tcccgtgctt gtgtcaggta ttctgtgcgc
    45181 atttattaaa aaaacacgta acttcgccag ttttactcgc gggtgctgtt gggtattggg
    45241 aatcctgctt ttatccaaag tcggcaatac gtttcgtatg tttaccccgt ggcaatatac
    45301 ctttgagaat gctgcgataa ccgtgactat cccgaaccga cactggaata cggtttccat
    45361 tagtactaat gaaaccattg atataagaag tgaggataat agcgtcttca tttctgcttt
    45421 ccgcctgccc gcaggacgta gcgccgatga cccgctggaa gcgttaaaga agaagcagcg
    45481 agacaatctc aaggatcaat acaatgagga agccttccag tttcatgttt gtaatgccaa
    45541 acatttcacc tgtaagtatc aggatgtgtt gataaatttt gatggtcagc agaaaagaac
    45601 catcagtgtt tatcttgaag acacccccag ggctgtgggg atcattgcat taatggcgcc
    45661 agatacggca gataaatatc gccaacaggc gatggagatt atgctttccg ctaaaaatgc
    45721 agtgaagtaa aaaataaggc gccttgcggc gcctctgcat ggaagagatt aaacctcttc
    45781 catgcgaccc agcagggcct gcagacgttc ctgccagccg ttctgctgtt ctttcagatg
    45841 gttgttctca cgctccagct cttcgcgctg atgctgggca ttttgaactt cctgcgacag
    45901 tgagttgttt ttttctttca gctcttcgat ttccatctgc aacagagtga tggtatcaat
    45961 cgcctgctgt acttttgctt ccagtttctc aaacacttct aatgacattg tcatacctct
    46021 cctgaattgc aaggcgttga tggataaaaa tcctcgtccc gattaccggt gacgccttaa
    46081 taaatacgag cgcactttag ttagctccga ttgtatgaag ccgcgccatc gctgtccagc
    46141 ggcacgcctt gcagattacg gtttgccaca cttttcatcc ttctcctggt gacataatcc
    46201 acatcaatcg aaaatgttaa taaatttgtt gcgcgaatga tctaacaaac atgcatcatg
    46261 tacaatcaga tggaataaat ggcgcgataa cgctcatttt atgacgaagc acacacattt
    46321 taagttcgat atttctcgtt tttgctcgtt aacgataagt ttacagcatg cctacaagca
    46381 tcgtggaggt ccgtgacttt cacgcataca acaaacatta actcttcagg atccgattat
    46441 gagtcaaaca tcaaccttga aaggccagtg cattgctgaa ttcctcggta ccgggttgtt
    46501 gatttttttc ggtgtgggtt gcgttgcagc actaaaagtc gctggtgcgt cttttggtca
    46561 gtgggaaatc agtgtcattt ggggactggg ggtggcaatg gccatctacc tgaccgcagg
    46621 ggtttccggc gcgcatctta atcccgctgt taccattgca ttgtggctgt ttgcctgttt
    46681 cgacaagcgc aaagttattc cttttatcgt ttcacaagtt gccggcgctt tctgcgctgc
    46741 ggctttagtt tacgggcttt actacaattt atttttcgac ttcgagcaga ctcatcacat
    46801 tgttcgcggc agcgttgaaa gtgttgatct ggctggcact ttctctactt accctaatcc
    46861 tcatatcaat tttgtgcagg ctttcgcagt tgagatggtg attaccgcta ttctgatggg
    46921 gctgatcctg gcgttaacgg acgatggcaa cggtgtacca cgcggccctt tggctccctt
    46981 gctgattggt ctactgattg cggtcattgg cgcatctatg ggtccattga cgggctttgc
    47041 catgaaccca gcgcgtgact tcggtccgaa agtctttgcc tggctggcgg gctggggcaa
    47101 tgtcgccttt accggcggca gagacattcc ttacttcctg gtgccgcttt ttggccctat
    47161 cgttggcgcg attgtaggtg catttgccta tcgcaaactg attggtcgcc atttgccttg
    47221 cgatatctgt gttgtggaag aaaaggagac cacaactcct tcagaacaaa aagcttcgct
    47281 gtaatatgac tacgggacaa ttaaacatga ctgaaaaaaa atatatcgtt gcgctcgacc
    47341 agggcaccac cagctcccgc gcggtcgtaa tggatcacga tgccaatatc attagcgtgt
    47401 cgcagcgcga atttgagcaa atctacccaa aaccaggctg ggtagaacac gacccaatgg
    47461 aaatctgggc cacccaaagc tccacgctgg tagaagtgct ggcgaaagcc gatatcagtt
    47521 ccgatcaaat tgcagctatc ggtattacga accagcgtga aaccactatt gtctgggaaa
    47581 aagaaaccgg caagcctatc tataacgcca ttgtctggca gtgccgtcgt accgcagaaa
    47641 tctgcgagca tttaaaacgt gacggtttag aagattatat ccgcagcaat accggtctgg
    47701 tgattgaccc gtacttttct ggcaccaaag tgaagtggat cctcgaccat gtggaaggct
    47761 ctcgcgagcg tgcgcgtcgt ggtgaattgc tgtttggtac ggttgatacg tggcttatct
    47821 ggaaaatgac tcagggccgt gtccatgtga ccgattacac caacgcctct cgtaccatgt
    47881 tgttcaacat ccataccctg gactgggacg acaaaatgct cgaggtgctg gatattccgc
    47941 gcgagatgct gccagaagtg cgtcgttctt ccgaagtata cggtcagact aacattggcg
    48001 gcaaaggcgg cacgcgtatt ccaatctccg ggatcgccgg tgaccagcag gcggcgctgt
    48061 ttggtcagtt gtgcgtgaaa gaagggatgg cgaagaacac ctatggcact ggctgcttta
    48121 tgctgatgaa cactggcgag aaagcggtga aatcagaaaa cggcctgctg accaccatcg
    48181 cctgtggccc aactggcgaa gtaaactatg cgctggaagg tgcggtgttt atggcgggcg
    48241 catccattca gtggctgcgt gatgagatga agttgattaa cgacgcctac gattccgaat
    48301 actttgccac gaaagtgcaa aacaccaatg gtgtgtatgt ggttccggcc tttaccgggc
    48361 tgggtgcgcc atactgggat ccgtatgcac gcggggcgat tttcggtctg actcgtgggg
    48421 tgaacgctaa ccacattata cgcgcgacgc tggagtctat tgcttatcag acgcgtgacg
    48481 tgctggaagc gatgcaggcc gattctggta ttcgtctgca cgccctgcgc gtggatggcg
    48541 gcgcagtggc gaacaatttc ctgatgcagt tccagtccga tattctcggt acgcgcgttg
    48601 agcgcccgga agtgcgcgaa gtcaccgcat tgggtgcggc ctatcttgct ggtctggcgg
    48661 ttggcttctg gcagaacctc gacgagctgc aagagaaagc ggtgattgag cgcgagttcc
    48721 gtccaggcat cgaaaccact gagcgtaatt accgttacgc aggctggaaa aaagcggtga
    48781 aacgcgcgat ggcgtgggaa gaacacgacg agtaatgtaa atgccggatg aagcgtttat
    48841 gccgcatccg gtagtcctga atcgtgcggg ggcaaccccg cacacatcaa taatcccgcc
    48901 cttcccctgt gctacacttc gcgccattcc ttactgctta gagtttgcta tgagacgaga
    48961 acttgccatc gaattttccc gcgtcaccga atctgcggcg ctggctggct acaaatggct
    49021 aggacgcggc gataaaaaca ccgcggacgg cgcagcggta aacgccatgc gtattatgct
    49081 caaccaggtc aacattgacg gcaccatcgt tattggtgaa ggtgaaatcg acgaagcacc
    49141 gatgctctac attggtgaaa aagtcggtac tggtcgcggc gacgcggtag atattgctgt
    49201 tgatccgatt gaaggcacgc gcatgacggc gatgggccag gctaacgcgc tggcggtgct
    49261 ggctgttggc gataaaggct gcttcctcaa tgcgccagat atgtatatgg agaagctgat
    49321 cgtcggaccg ggagccaaag gcaccattga tctgaacctg ccgctggcgg ataacctgcg
    49381 caatgttgcg gcggcgctcg gtaaaccgtt gagcgaactg acggtaacaa ttctggctaa
    49441 accacgccac gatgccgtta tcgctgaaat gcagcaactc ggcgtacgcg tatttgctat
    49501 tccggacggc gacgttgcgg cctcaattct cacctgtatg ccagacagcg aagttgacgt
    49561 gctgtacggt attggtggcg cgccggaagg cgtggtttct gcagcggtga tccgcgcatt
    49621 agatggtgac atgaacggtc gtctgctggc gcgtcatgac gtcaaaggcg acaacgaaga
    49681 gaatcgtcgc attggcgagc aggagctggc acgctgcaaa gcaatgggca tcgaagccgg
    49741 taaagtattg cgtctgggcg atatggcgcg cagcgataac gtcatcttct ctgccaccgg
    49801 tattaccaaa ggcgatctgc tggaaggcat tagccgcaaa ggcaatatcg cgactaccga
    49861 aacgctgctg atccgcggca agtcacgcac tattcgccgc attcagtcca tccactatct
    49921 ggatcgcaaa gacccggaaa tgcaggtgca cattctctga gtgatttgat cgattgagcc
    49981 ttccagtcct tcgggactgg aatttttttg ttcggagaac gaagataagg caagtcaatc
    50041 aaaacaggag aaaaacatgg ctgattgggt aacaggcaaa gtcactaaag tgcagaactg
    50101 gaccgacgcc ctgtttagtc tcaccgttca cgcccccgtg cttccgttta ccgccgggca
    50161 atttaccaag cttggccttg aaatcgacgg tgaacgcgtc cagcgcgcct actcctatgt
    50221 taactcgccc gataatcccg atctggagtt ttacctggtc accgtccccg atggcaaatt
    50281 aagcccacga ttggcggcac tgaaaccggg cgatgaagtg caggtggtta gcgaagcagc
    50341 tggcttcttt gttctggatg aagtaccaga ttgcgaaacg ctatggatgc tggcaaccgg
    50401 tacagcgatt ggcccttatc tatcgattct gcaactgggt aaagatttag atcgcttcaa
    50461 aaatctggtc ctggttcacg ccgcacgtta tgccgccgac ttaagctatt tgccactgat
    50521 gcaagaactg gaaaaacgct acgaaggcaa actgcgcatt cagacggtgg tcagtcggga
    50581 aacggctgcg ggttcgctca ctgggcggat accggcgtta attgaaagtg gtgaactgga
    50641 aagcgcggtt ggcctgccga tgaataaaga aaccagccat gtgatgctgt gcggcaatcc
    50701 acagatggtg cgcgatacgc aacagttgct gaaagagacc cggcagatga cgaaacattt
    50761 acgtcgccga ccgggccata tgacagcgga gcattactgg taagcggtta cttatcgata
    50821 aacggcacga tgagcaaatc cgcactcaac ttattgatca tcccgcgata tgccggcatc
    50881 aaacggttga taaatgagtg gtgatgacca cagacaagga ggtcgcactg ctctttttgc
    50941 ataatttcca gcagtgtttc cggcatttct ccgcgttcaa tacgcagttt tgtcttcggc
    51001 cattgaatat ttttcgtcag tttatacagc ttgttatccg acttattctt caacaattga
    51061 agaatatctt ctgttgcagg gaagtagata cccggataca actcgcttaa gccatcatca
    51121 atatgaatta acgtcaggtg agcgtcatta tgtctggcga gctccagggc tttattcacc
    51181 agtaaggcat cttcttcatt cccggaaatt gccacgccaa tgtgtttata agccatgttt
    51241 aactccttct aaagcaactc catcaagcta acgaacgcag tgataactca aaaataatca
    51301 tctcggcctg gcatgagaag gtgaaggccg catcaagctc aacttgccct tccagatcct
    51361 taatcttata ggctacgctg gccccttcat ctgcagagga tgcagcggcg gcaagctctt
    51421 tcagtttggt cataaaggac tctgcttctt cgcgggtggc aaaaaagcgc gaaaacttac
    51481 tggtacagtt gtcgttatca atcaccgtac cgatatcaat cgcacatcct ttagtactgc
    51541 atttatctac gacatctttc atagggcacc tctgtatatt cgcctctctg tcgattacga
    51601 cttaagaata atgcgccgtt aaaggaagaa caaacaagta atgatcttat ggattaccat
    51661 tttttttgat attgcttaag gtgaagagtt cagaaggatt ttccgggcat tgtcacttgc
    51721 atcgtggaaa taacaatccc ggaaaaaaat agcagtaaga gatatattaa aggttaatta
    51781 cttgatttat tgtcggcttt atacttcaca tcctgggtat ctttaccata tttattttcg
    51841 ccttgtgtgc caacaaacgc accaagatcg ataagcatca tcaccagaat caatgtcggg
    51901 acaaaacgcc ccaccgccca ttgccagaca cccggtaaaa tcgcccagtt acccgccagc
    51961 agcatccacg ccacaatcat cagaaatgcc catgcgccgg aacgcccgcg atcatgcaag
    52021 cgcttaacgg ttactgccgc tgttggccaa agcaagcaca caaggcaaaa cgccgcggtc
    52081 tgaatatcga gtaaattttt acccgccagt gagaaaagca ccagcatgcc tgcgaaccac
    52141 aggcctatcc aaatccagaa atcacggcgt ccaatacgcc ctttaaatga gaataaccat
    52201 tgctgtatgg tcatgtaagt tccttgatgg ttgtcttttc caggattcta cccttttgac
    52261 aagggcgaca gatatcgttt taatcggagc cagtcataac aaaaggtact gtcaatgaag
    52321 ccagggtgta cgctgttttt tctcttatat tctgcattaa ccgttacaac aacggcgcat
    52381 gcacaaacac cagatacggc aacgaccgcg ccttatctgc tggctggagc ccctactttc
    52441 gatctctcca tcagccagtt tcgagaagac tttaacagcc agaatcccag cctgccactg
    52501 aacgaatttc gtgccatcga cagcagtccc gacaaagcca atctcactcg tgctgccagt
    52561 aaaattaacg agaacttgta tgcttctaca gcgctggagc gcggtacctt aaaaatcaaa
    52621 agcattcaaa tgacctggct acccatccag gggccagagc aaaaagccgc gaaagcgaaa
    52681 gctcaggaat acatggcagc ggtgatccgc acactcaccc cattaatgac caaaacacaa
    52741 agccagaaaa aactgcagtc gctactaacg gcggggaaaa acaaacgtta ttacaccgag
    52801 acagaaggtg cactgcgtta tgttgtcgcg gacaacggcg aaaaggggct gaccttcgct
    52861 gttgaaccga ttaagctggc actatctgaa tcgcttgaag gtttgaataa atgacaaaaa
    52921 gcaaagcctt tgtgccgatg aatctctata ctgtttcaca gacctgctgc cctgcggggc
    52981 ggccatcttc ctttattcgc ttataagcgt ggagaattaa aatgcgacat cctttagtga
    53041 tgggtaactg gaaactgaac ggcagccgcc acatggttca cgagctggtt tctaacctgc
    53101 gtaaagagct ggcaggtgtt gctggctgtg cggttgcaat cgcaccaccg gaaatgtaca
    53161 tcgatatggc gaagcgcgaa gctgaaggca gccacatcat gctgggtgcg caaaacgtgg
    53221 acctgaacct gtccggcgca ttcaccggtg aaacctctgc tgctatgctg aaagacatcg
    53281 gcgcacagta catcatcatc ggccactctg aacgtcgtac ttaccacaaa gagtctgacg
    53341 aactgatcgc gaaaaaattc gcggtgctga aagagcaggg actgactccg gttctgtgca
    53401 tcggtgaaac cgaagcggaa aacgaagcgg gcaaaactga agaagtttgt gcacgtcaga
    53461 tcgacgcggt actgaaaact cagggggctg cggcattcga aggtgcggtt atcgcttacg
    53521 aacctgtatg ggcaatcggt actggcaaat ctgcaactcc ggctcaggca caggctgttc
    53581 acaaattcat ccgtgaccac atcgctaaag ttgacgctaa catcgctgaa caagtgatca
    53641 tccagtacgg cggctctgta aacgcgtcta acgctgcaga actgtttgct cagccagaca
    53701 tcgacggcgc gctggttggc ggtgcttctc tgaaagctga cgctttcgca gtgatcgtta
    53761 aagctgcaga agcggctaaa caggcttaag tctgacaggt gccggatttc atatccggca
    53821 cttactttcc ttaactcctc gccttaacgc aaaatctcac actgatgatc ctggatttcc
    53881 tcggctgaag cacggttcag cgtcagtaga tttcgttgtg tcgccagcaa tacaaatgag
    53941 ttatcactct gccgtaccat tgccagcccg tagcttccca tatgttcccg cgcctcaggc
    54001 acttcttctg caagcatcat aaatgggctg cgttgtacca gttcgctttc cgttacccga
    54061 cgcgccaggt actcatgccc acgcaaacca cctggcagtg gcaaccagcg gctactgatg
    54121 ttcgccagat tgttatccag ctgttcgcgc acatcaggac gaatacaaga aatatgaata
    54181 tgaaaatggt tttgcgtacg cccggtgcga gagttgatcg ccaaagaaac cgcgcgatcg
    54241 ggaaccggct ggccgtattt tttgctcata aaatcacgcg cctgccaggc caaccaaaag
    54301 aagttcggcg ttgaaggatc ggtcaacaaa ggactttcag taccgttaat acgatacgtt
    54361 ggcatcaaca gatattgcag tgggccatta agatctttta aaaccacgta tccggcgttg
    54421 ggtttgactt ccgcacatgg cgaaggattt tgattttgct gctgattggg caaacattcc
    54481 tcaaggacaa tcttacgtaa tgtatccgac tcttcaccgg ttaatttcca gtaaccaata
    54541 ccggcagcca caacggcgat aactatcatc accaaaaaaa gaagacccgc ttttttcatc
    54601 tttttttccc tgtacctcaa agagatgcaa gggtaacgca aaatcgtgac aaataaaaaa
    54661 cccggtcgcg agaaaccacc gggtttgata attatcctgg agaaatcagc gtttgctgat
    54721 ctgatcgaac gtaccgccgt tagcaaaatg ctctttttgc gctttcgtcc agccgccgaa
    54781 ctcttcatca atggtgaata acttcagctt tggaaacgca ttttcgtact ttttcgccac
    54841 ctcagcatcg cgcggacggt agtagttttt cgcggcaatt tcctgacctt ctggcgagta
    54901 gagatatttc aggtaggctt ccgccacctc tttggtgcct tttttctcga ccactttatc
    54961 gaccaccgac acggttggct ctgcgaggat agactcactc ggcgtgacga tttcgaattt
    55021 atctttcccc agttcattcg ctgccagcag agcttcgttt tcccaggcga tcagtacatc
    55081 gccaattccg cgctcgacaa aagtgttagt ggagccgcgc gcgccagaat ccagaacttc
    55141 gacgttttta tacagtgccc gaacaaaatc ctgtgctttt gcctgatcgt tgttgttgtg
    55201 atgcagcgcg tagccccagg cagccaggta gttccagcgc gcgccaccag agcttttcgg
    55261 attaggcgtg atcaccgaaa cacccggttt aatcagatcg ttccagtcat ggatctgctt
    55321 cggattgccc ttacgtacca ggaaaacaat ggtggaagtg tacggtgcgg agttatccgg
    55381 cagacgtttg atccactctt tatcaatccg cccgcgttcc gcaattgcgt ccacgtcata
    55441 ggccagagcc agagtgacaa catcagcttc aataccattg attaccgacg tcgcttgttt
    55501 gcctgaacca ccgtgggact gacggatcac cacgttatcg ccagtttgct gtttccagtg
    55561 ggcgctgaat gccttgttgt actgttcgta caattcgcgc gttggatcat atgaaacgtt
    55621 aagaagctga atatcctttg ccataacgct ggttgccgcc agcaaaaatg ttaaccctac
    55681 gccccacttg ttcatcgccc gactctctta tgttgtgttg tgatgagcaa agcgtgccag
    55741 aaggttaacc aaacattaaa gaataaaaaa agattggcta taacttgcgg gtatatgttg
    55801 agggattaaa aaggcggaag aaatccgcct catattgctg acaaagtgcg ctttgtccat
    55861 gccggatgcg gcgcgaacgc cttatccggc ctacaaaagt ttgcaaattc aataaattgc
    55921 agaattcatg taggcctgat aagcgaagcg catcaggcat ttttgcttct gtcatcggtt
    55981 tcaggctaaa ggaatctgcc tttttccgaa atcattaata cagttttttc gcgcagtcca
    56041 gccagtcgcc tttgaacgga cgcttcatgt tttcgatagc gtcgatgatg tcgtggtgaa
    56101 ccagctgttc gttctggata cctacgcaac gaccgccgta gcctgccagc agcagatcga
    56161 tagcgtaagc gcccatacgg gaagccagaa tacggtcgta aggcaccgga gaaccaccgc
    56221 gctggatgtg gcccagcaca gttgcgcggg tttcacgacc ggtttctttc tcgatgaaat
    56281 gcgccagttc gtcaacatca cacatatgtt cggtaatcgc cacgatcgcg tgttttttac
    56341 ctttcgcgat acccgctttg atttcgttta ccaggtcttc acggctgaat tcaacttccg
    56401 gaactacaac gaattcacag ccaccagcaa tagccgcagc caacgtcaga tcgccacaat
    56461 aacgacccat cacttccacc acggaaatac gctggtgaga agaagaggtg tcacgcagac
    56521 ggtcgatcgc ttctacaacg gtgctcagcg cagtgaagaa accgatagtg tagtcagtgc
    56581 ctttgatgtc gttgtcgata gtgcccggca ggccgatgca cgggaagccc atttcggtca
    56641 gacgcattgc acccatgtag gaaccgtcac cgccgataac caccagcgca tcgatcccac
    56701 gttttttcag gttttcgata gccacggcgc ggatgttctc gtcgcggaat tccgggaaac
    56761 gcgcagaacc gaggaacgta ccgccacggt tgatcatgtc agaaacgctg taacggtcta
    56821 gctgtaccat acggtcttca tacagaccca gatagccgtc ataaatgccc attacttcca
    56881 gaccttctgt cagcgcagaa cgaacaaccc cgcgaattgc ggcgttcatg cctggcgcat
    56941 caccgccgct tgtcaacaca ccgattttct taatcatgac tacctctgaa ctttggaatg
    57001 caaaatgaaa tctgttgccg gaagtcttct tgcacatcga agtgatccaa cgaatgtgca
    57061 aatagtataa caatcacttc ctgctgaatt gattcaggtc aggccaaatg gcggtatttt
    57121 atacacaaaa tgcgggtctg gctctctttt atactgatta tgaaagcata gaccgtttac
    57181 cctccctggg tacgacggaa caggggtcct gatggataat cacatccgat cccggaaaac
    57241 gccgtaaaat agcctgctct acctgatccg ccaccatatg tgcctgaacc aaaggcagag
    57301 agtcttccat ttccaaatga atctgaataa agcgggtcgg ccctgactgc cgcgtgcgaa
    57361 gatcgtgagc gccgctaaca cccggccagg aagtcacgat atcaataatt tcttgccgtt
    57421 cttcatcagg caatgcgcga tccagtaatg actgtaccgc ctcatatccc atgcgtaacg
    57481 cgctatataa aatatagatg ccgattccca atgcaaacag agcatcggcg cgatgccagc
    57541 cgtaccagga caaccccagc gccagcagaa ttgcgccgtt catcataaca tcagactggt
    57601 aatgtagcat atcagcccgc accgcctggc tttgcgtccg gcgcaccacc caccgctgaa
    57661 acgagacaag gataatcgta caaattagcg ccacaattgt cacgataacc ccgacgcctg
    57721 gatctgtcat cggtgttgga gataccagat gttgaatacc cgtcaaaaac aggaatagtg
    57781 ccgaaccgga gataaacata ctttgcgcca gcgccgcgag ggactcagct ttaccgtgac
    57841 caaacgagtg attatcgtcg gcaggttgca gggaatatcg caccaccagt aaattcgtca
    57901 acgacgcgcc gatatccacc agcgaatcca ccagcgcggc gagaatactc accgacccgg
    57961 tataccacca tgcaaaaatt ttaatcagca atagcagcga agccatcgcc gtcgcagcaa
    58021 tcgccgcccg actgaccagc cgtccataag attgattcat aaatactccc gctatcaact
    58081 gacggtagta taacggaagc aaatcatctg caatgcatta agcagcaagc aaattgagga
    58141 taaaaaaaac ccccacatca tgtgggggaa gacagggatg gtgtcacaaa aaatcaccta
    58201 acctactgat ataaatggat ttatataaac cactgtccac atagcgtcca catcgaccat
    58261 aaataaagcc cctcaactga gg
//
LOCUS       DAMZFT010000014        57730 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.014, whole genome shotgun sequence.
ACCESSION   DAMZFT010000014 DAMZFT010000000
VERSION     DAMZFT010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 57730)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 57730)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..57730
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.014"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(<1..731)
                     /locus_tag="PN977_000993"
     CDS             complement(<1..731)
                     /locus_tag="PN977_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HDI6041056.1"
                     /translation="MHVTAKPSSFQCNLKCDYCFYLEKESQFTHEKWMDDSTLKEFIK
                     QYIAASGNQVYFTWQGGEPTLAGLDFFRKVIHYQQRYAGQKRIFNALQTNGILLNNEW
                     CAFLKEHEFLVGISIDGPQELHDRYRRSNSGNGTFAKVIAAIERLKSYQVEFNTLTVI
                     NNVNVHYPLEVYHFLKSIGSKHMQFIELLETGTPNIDFSGHSENTFRIIDFSVPPTAY
                     GKFMSTIFMQWVKNDVGEIFIRQFE"
     gene            complement(783..2465)
                     /locus_tag="PN977_000994"
     CDS             complement(783..2465)
                     /locus_tag="PN977_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310130.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HDI6041057.1"
                     /translation="MKSALKKSVVSTSISLILASGMAAFAAHAADDVKLKATKTNVAF
                     SDFTPTEYSTKGKPNIIVLTMDDLGYGQLPFDKGSFDPKTMENREVVDTYKIGIDKAI
                     EAAQKSTPTLLSLMDEGVRFTNGYVAHGVSGPSRAAIMTGRAPARFGVYSNTDAQDGI
                     PLTETFLPELFQNHGYYTAAVGKWHLSKISNVPVPEDKQTRDYHDNFTTFSAEEWQPQ
                     NRGFDYFMGFHAAGTAYYNSPSLFKNRERVPAKGYISDQLTDEAIGVVDRAKTLDQPF
                     MLYLAYNAPHLPNDNPAPEQYQKQFNTGSQTADNYYASVYSVDQGVKRILEQLKKNGQ
                     YDNTIILFTSDNGAVIDGPLPLNGAQKGYKSQTYPGGTHTPMFMWWKGKLQPGNYDKL
                     ISAMDFYPTALDAADISIPKDLKLDGVSLLPWLQDKKQGEPHKNLTWITSYSHWFDEE
                     NIPFWDNYHKFVRHQSDDYPHNPNTEDLSQFSYTVRNNDYSLVYTVENNQLGLYKLTD
                     LQQKDNLAAANPQVVKEMQGVVREFIDSSQPPLSEVNQEKFNNIKKALSEAK"
     gene            complement(2866..3627)
                     /gene="ydeO"
                     /locus_tag="PN977_000995"
     CDS             complement(2866..3627)
                     /gene="ydeO"
                     /locus_tag="PN977_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response transcriptional regulator
                     YdeO"
                     /protein_id="HDI6041058.1"
                     /translation="MSLVCSVIFIHHAFNANILDKDYAFSDGEILMVDNAVRTHFEPY
                     ERHFKEIGFNENTIKKYLQCTNIQTVTVPVPAKFLRASNVPTGLLNEMIAYLNSEERN
                     HHNFSELLLFSCLSIFAACKGFITLLTNGVLSVSGKVRNIVNMKLAHPWKLKDICDCL
                     YISESLLKKKLKQEQTTFSQILLDARMQHAKNLIRVEGSVNKIAEQCGYASTSYFIYA
                     FRKHFGNSPKRVSKEYRCQRHTGMNTDNTMSALAI"
     gene            complement(3702..3899)
                     /locus_tag="PN977_000996"
     CDS             complement(3702..3899)
                     /locus_tag="PN977_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component-system connector protein SafA"
                     /protein_id="HDI6041059.1"
                     /translation="MHATTVKNKIMQRDNYKEIMSVIVVVLLLTLTLIAIFSAIDQQG
                     ISEMGRMARDLTHFIINSLQD"
     gene            complement(4147..6426)
                     /gene="ydeP"
                     /locus_tag="PN977_000997"
     CDS             complement(4147..6426)
                     /gene="ydeP"
                     /locus_tag="PN977_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance putative oxidoreductase YdeP"
                     /protein_id="HDI6041060.1"
                     /translation="MKKKIESYQGAAGGWGAVKSVANAVRKQMDIRQDVIAMFDMNKP
                     EGFDCPGCAWPDPKHSASFDICENGAKAIAWEVTDKQVNASFFAENTVQSLLTWGDHE
                     LEAAGRLTQPLKYDAVSDCYKPLSWQQAFDEIGERLQSYSDPNQVEFYTSGRTSNEAA
                     FLYQLFAREYGSNNFPDCSNMCHEPTSVGLAASIGVGKGTVLLEDFEKCDLVICIGHN
                     PGTNHPRMLTSLRALVKRGAKMIAINPLQERGLERFTAPQNPFEMLTNSETQLASAYY
                     NVRIGGDMALLKGMMRLLIERDDAASAAGRPSLLDDEFIQTHTVGFDELRRDVLNSEW
                     KDIERISGLSQTQIAELADAYAAAERTIICYGMGITQHEHGTQNVQQLVNLLLMKGNI
                     GKPGAGICPLRGHSNVQGDRTVGITEKPSAEFLDRLGERYGFTPPHAPGHAAIASMQA
                     ICTGQARALICMGGNFALAMPDREASAVPLTQLDLAVHVATKLNRSHLLTARHSYILP
                     VLGRSEIDMQKSGAQAVTVEDSMSMIHASRGVLKPAGVMLKSECAVVAGIAQAALPQS
                     VVAWEYLVEDYDRIRNDIEAVLPEFADYNQRIRHPGGFHLINAAAERRWMTPSGKANF
                     ITSKGLLEDPSSAFNSKLVMATVRSHDQYNTTIYGMDDRYRGVFGQRDVVFMSAKQAK
                     ICRVKNGERVNLIALTPDGKRSSRRMDRLKVVIYPMADRSLVTYFPESNHMLTLDNHD
                     PLSGIPGYKSIPVELEPSN"
     gene            complement(6765..7679)
                     /locus_tag="PN977_000998"
     CDS             complement(6765..7679)
                     /locus_tag="PN977_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HDI6041061.1"
                     /translation="MGKTISMKLLFGIYLLLMAGKVFAFSCNVDGGSSIGAGTTSVYV
                     NLDPVIQPGQNLVVDLSQHISCWNDYGGWYDTDHINLVQGSAFAGSLQSYKGSLYWNN
                     VTYPFPLTTNTNVLDIGDKTPMPLPLKLYITPVGAAGGVVIKAGEVIARIHMYKIATL
                     GSGNPRNFTWNIISNNSVVMPTGGCTVDSRNVNVNLPDFPGSAEIPLGVYCSSEQKLS
                     FYLSGATTDSARQVFANTAPDATKASGVGVSLMRNGKILATGENVSLGTVNKSKVPLG
                     LSATYGQTGNKVAAGTVQSVIGVTFIYE"
     gene            complement(7739..8242)
                     /locus_tag="PN977_000999"
     CDS             complement(7739..8242)
                     /locus_tag="PN977_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HDI6041062.1"
                     /translation="MKRLHKRFLLATFCALFTATLQAADVTITVIGRVVAKPCTIQTK
                     EANVNLGDLYTRNLQQPGSASGWHNITLSLTDCPVETSAVTAIVTGSTDNTGYYKNEG
                     TAENIQIELRDDQDATLKNGDSKTVIVDEITRNAQFPLKARAITVNGNASQGTIEALI
                     NVIYTWQ"
     gene            complement(8255..8785)
                     /locus_tag="PN977_001000"
     CDS             complement(8255..8785)
                     /locus_tag="PN977_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HDI6041063.1"
                     /translation="MKYNNVIFLGLCLGLTTYSALSADSVIKISGRVLDYGCTVSSDS
                     LNFTVDLQKNSARQFPTTGSTSPAVPFQITLSECSKGTTGVRVAFNGIEDAENNTLLK
                     LDEGSNTASGLGIEILDGNMRPVKLNDLHAGMQWIPLVPEQNNILPYSARLKSTQKSV
                     NPGLVSASATFTLEFQ"
     gene            complement(8799..11450)
                     /locus_tag="PN977_001001"
     CDS             complement(8799..11450)
                     /locus_tag="PN977_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HDI6041064.1"
                     /translation="MTAFRAAFKAYRMHQVLLLPRFARLTIALGLATAVFPVDAEFYF
                     NPRFLSNDLAESVDLSAFTKGREAPPGTYRVDIYLNDEFMTSRDITFIADDNNADLIP
                     CLSTDLLVSLGIKKSALLDNKEHSAEKHVSDNSACTPLRDRLADASSEFNVGQQHLSL
                     SVPQIYVGRMARGYVSPDLWEEGINAGLLNYSFNGNSINNRGNHNAGKSNYAYLNLQS
                     GINIGSWRLRDNSTWSYNSGSSNSSDSNKWQHINTSAERDIIPLRSRLTVGDSYTDGD
                     IFDSVNFRGLKINSTEAMLPDSQHGFAPVIHGIARSTAQVSVKQNGYDVYQTTVPPGP
                     FTIDDINSAANGGDLQVTIKEADGSIQTLYVPYSSVPVLQRAGYTRYALAMGEYRSGN
                     NLQSSPKFIQGSLMHGLEGNWTPYGGMQIAEDYQAFNLGIGKDLGLFGAFSFDITQAN
                     TTLADGTRHSGQSIKSVYSKSFYQTGTNIQVAGYRYSTQGFYNLSDSAYSRMSGYTVK
                     PPTGDSNEQTQFIDYFNLFYSKRGQEQISISQQLGNYGTTFFSASRQSYWNTSRSDQQ
                     ISFGLNVPFSDITTSLNYSYSNNIWQNDRDHLLAFTLNVPFSHWMRTDSQSAFRNSNA
                     SYSMSNDLKGGMTNLSGVYGTLLPDNNLNYSVQVGNTHGGNTSSGTSGYSSLNYRGAY
                     GNTNIGYSRSGDSSQIYYGMSGGIIAHADGITFGQPLGDTMVLVKAPGADNVKIENQT
                     GIHTDWRGYAILPFATEYRENRVALNANSLADNVELDETVVTVIPTHGAIARATFNAQ
                     IGGKVLMTLKYGNKSVPFGAIVTHGENKNGSIVAENGQVYLTGLPQSGKLQVSWGNDK
                     NSNCIVDYKLPAVSPGTLLNQQTAICR"
     gene            complement(11492..12202)
                     /locus_tag="PN977_001002"
     CDS             complement(11492..12202)
                     /locus_tag="PN977_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HDI6041065.1"
                     /translation="MQTTRIPYSISFMATVLLLLLFACHSTIANAAVALGATRVIYPA
                     NQKQVLLPVTNNDPASVYLIQSWIENAGDQKDTQFVITPPLFSMQGKKENTLRIINAT
                     NHQLPGDRESLFWVNVKAIPAMEKDQKNENTLQLAIISRIKMFYRPTNLAMAPEEAPA
                     MLRFRRSGSKLTLINPTPYFITVTNMKAGNSNLPNTMVPPKGEVSVDIPHAATGDISF
                     QTINDYGALTPRIKATMQ"
     gene            complement(12565..13128)
                     /gene="fimA"
                     /locus_tag="PN977_001003"
     CDS             complement(12565..13128)
                     /gene="fimA"
                     /locus_tag="PN977_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HDI6041066.1"
                     /translation="MKLKHVGMIVVSVLAMSSAAVSAAEGDESVTTTVNGGVIHFKGE
                     VVNAACAIDSESMNQTVELGQVRSSRLAKAGDLSSAVGFNIKLNDCDTNVSSNAAVAF
                     LGTTVTSNDDTLALQSSAAGSAQNVGIQILDRTGEVLILDGATFSAKTDLIDGTNILP
                     FQARYIALGQSVAGTANADATFKVQYL"
     gene            complement(13562..13828)
                     /locus_tag="PN977_001004"
                     /pseudo
     CDS             complement(13562..13828)
                     /locus_tag="PN977_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226947.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            complement(14271..19069)
                     /locus_tag="PN977_001005"
                     /pseudo
     CDS             complement(14271..19069)
                     /locus_tag="PN977_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001475310.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter barrel domain-containing
                     lipoprotein"
     gene            complement(19136..19207)
                     /locus_tag="PN977_001006"
                     /pseudo
     CDS             complement(19136..19207)
                     /locus_tag="PN977_001006"
                     /inference="COORDINATES: protein motif:HMM:NF024418.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(19737..21329)
                     /gene="lsrK"
                     /locus_tag="PN977_001007"
     CDS             complement(19737..21329)
                     /gene="lsrK"
                     /locus_tag="PN977_001007"
                     /EC_number="2.7.1.189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416028.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer-2 kinase"
                     /protein_id="HDI6041067.1"
                     /translation="MARLFTPSESKYYLMALDAGTGSIRAVIFDLEGNQIAVGQAEWR
                     HLAVPDVPGSMEFDLNKNWQLACECMRQALHNAGIAPEYIAAVSACSMREGIVLYNND
                     GTPIWACANVDARAAREVSELKELHNNTFENKVYRATGQTLALSAIPRLLWLAHHRSD
                     IYRQASTITMISDWLAYMLSGELAVDPSNAGTTGLLDLTTRDWKPALLDMAGLRADIL
                     SPVKETGTLLGVVSSHAAELCGLKAGTPVVVGGGDVQLGCLGLGVVRPAQTAVLGGTF
                     WQQVVNLAAPVTDPEMNVRVNPHVIPGMVQAESISFFTGLTMRWFRDAFCAEEKLIAE
                     RLGIDTYTLLEEMASRVPPGSWGVMPIFSDRMRFKTWYHAAPSFINLSIDPDKCNKAT
                     LFRALEENASIVSACNLQQIADFSNIHPTSLVFAGGGSKGKLWSQILADVSGLPVNIP
                     VVKEATALGCAIAAGVGAGIFSSMAETGERLVRWERTHTPDPEKHELYQDSRDKWQAV
                     YQDQLGLVDHGLTTSLWKAPGL"
     gene            complement(21408..22361)
                     /gene="lsrR"
                     /locus_tag="PN977_001008"
     CDS             complement(21408..22361)
                     /gene="lsrR"
                     /locus_tag="PN977_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LsrR"
                     /protein_id="HDI6041068.1"
                     /translation="MTINDSVISEQGMCEEEQVARIAWFYYHDGLTQSEISDRLGLTR
                     LKVSRLLEKGHQSGIIRVQINSRFEGCLEYETQLCRQFSLQHVRVIPGLADADVGGRL
                     GIGAAHMLMSLLQPQQMLAIGFGEATMNTLQRLSGFISSQQIRLVTLSGGVGSYMTGI
                     GQLNAACSVNIIPAPLRASSADIAHTLKNENCVKDVLLAAQAADVAIVGIGAVSQQDD
                     ATIIRSGYISQGEQLMIGRKGAVGDILGYFFDAKGDVVTDIKIHNELIGLPLSALKTI
                     PVRVGVAGGENKAEAIAAAMKGGYINALVTDQDTAAAILRS"
     gene            22610..24145
                     /gene="lsrA"
                     /locus_tag="PN977_001009"
     CDS             22610..24145
                     /gene="lsrA"
                     /locus_tag="PN977_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter ATP-binding
                     protein LsrA"
                     /protein_id="HDI6041069.1"
                     /translation="MQTSDTRALPLLCARSVYKQYSGVNVLKGIDFTLHQGEVHALLG
                     GNGAGKSTLMKIIAGITPADSGTLEIGGNNYVRLTPVHAHQLGIYLVPQEPLLFPSLS
                     IKENILFGLAKKQLSIQKMNNLLAALGCQFDLHSLAGSLDVADRQMVEILRGLMRDSR
                     ILILDEPTASLTPAETERLFIRLRELLATGVGIVFISHKLPEIRQIADRISVMRDGTI
                     ALSGKTSELSTDDIIQAITPALREKSLSASQKLWLELPGNRPQHAAGTPVLTLENLTG
                     EGFRNVSLTLNAGEILGLAGLVGAGRTELAETLYGLRTLRGGRIMLNGKEINRLSTGE
                     RLLRGLVYLPEDRQSSGLNLDASLAWNVCALTHNLRGFWAKTAKDNATLERYRRALNI
                     KFNQPEQAARTLSGGNQQKILIAKCLEASPQVLIVDEPTRGVDVSARNDIYQLLRSIA
                     AQNVAVLLISSDLEEIELMADRVYVMHQGEIAHSALTGRDINVETIMRVAFGDSQRQE
                     ASC"
     gene            24139..25167
                     /gene="lsrC"
                     /locus_tag="PN977_001010"
     CDS             24139..25167
                     /gene="lsrC"
                     /locus_tag="PN977_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter permease LsrC"
                     /protein_id="HDI6041070.1"
                     /translation="MLKFIQNNREITALLAVVLLFVLPGFLDRQYLSVQTLTMVYSSA
                     QILILLAMGATLVMLTRNIDVSVGSITGMCAVLLGMLLNAGYSLPVACVATLLLGLLA
                     GFFNGVLVAWLKIPAIVATLGTLGLYRGIMLLWTGGKWIEGLPAELKQLSAPLLFGVS
                     AIGWLTIILVAFMAWLLAKTAFGRSFYVTGDNLQGARQLGVRTEAIRIVAFSLNGCMA
                     ALAGIVFASQIGFIPNQTGTGLEMKAIAACVLGGISLLGGSGAIIGAVLGAWFLTQID
                     SVLVLLRIPAWWNDFIAGMVLLAVLVFDGRLRCALERNLRRQKYARFMMPPPPVKPAS
                     SGKKREAA"
     gene            25167..26159
                     /gene="lsrD"
                     /locus_tag="PN977_001011"
     CDS             25167..26159
                     /gene="lsrD"
                     /locus_tag="PN977_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter permease LsrD"
                     /protein_id="HDI6041071.1"
                     /translation="MRIRYGWELALAALLVIEIVAFGAINPRMLDLNMLLFSTSDFIC
                     IGIVALPLTMVIVSGGIDISFGSTIGLCAIALGVLFQSGVPMPLAILLTLLLGALCGL
                     INAGLIIYTKVNPLVITLGTLYLFAGSALLLSGMAGATGYEGIGGFPMAFTDFANLDV
                     LGLPVPLIIFLICLLVFWLWLHKTHAGRNVFLIGQSPRVAVYSAIPVNRTLCALYAMT
                     GLASAVAAVLLVSYFGSARSDLGASFLMPAITAVVLGGANIYGGSGSIIGTAIAVLLV
                     GYLQQGLQMAGVPNQVSSALSGALLIVVVVGRSVSLHRQQIKEWLARRANNPLP"
     gene            26171..27193
                     /gene="lsrB"
                     /locus_tag="PN977_001012"
     CDS             26171..27193
                     /gene="lsrB"
                     /locus_tag="PN977_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter substrate-binding
                     protein LsrB"
                     /protein_id="HDI6041072.1"
                     /translation="MTLHRFKKIALLSALGIAAISMNVQAAERIAFIPKLVGVGFFTS
                     GGNGAQQAGKELGVDVTYDGPTEPSVSGQVQLINNFVNQGYNAIIVSAVSPDGLCPAL
                     KRAMQRGVRVLTWDSDTKPECRSYYINQGTPAQLGGMLVDMAARQVNKDKAKVAFFYS
                     SPTVTDQNQWVKEAKAKIAKEHPGWEIVTTQFGYNDATKSLQTAEGILKAYSDLDAII
                     APDANALPAAAQAAENLKNDKVAIVGFSTPNVMRPYVERGTVKEFGLWDVVQQGKISV
                     YVADALLKKGSMKTGDKLDIQGVGQVEVSPNSVQGYDYEADGNGIVLLPERVIFNKEN
                     IGKYDF"
     gene            27220..28095
                     /gene="lsrF"
                     /locus_tag="PN977_001013"
     CDS             27220..28095
                     /gene="lsrF"
                     /locus_tag="PN977_001013"
                     /EC_number="2.3.1.245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxy-5-phosphonooxypentane-2,4-dione
                     thiolase"
                     /protein_id="HDI6041073.1"
                     /translation="MADLDDIKDGKDFRTDQPQQNIPFTLKGCGALDWGMQSRLSRIF
                     NSKTGKTVMLAFDHGYFQGPTTGLERIDISIAPLFEHADVLMCTRGILRSVVPPATNK
                     PVVLRASGANSILAELSNEAVALSMDDAVRLNSCAVAAQVYIGSEYEHQSIKNIIQLV
                     DAGMKVGMPTMAVTGVGKDMVRDQRYFSLATRIAAEMGAQIIKTYYVDKGFERIVAGC
                     PVPIVIAGGKKLPEREALEMCWQAIDQGASGVDMGRNIFQSDHPVAMMKAVQAVVHHN
                     ETADRAYELYLSEKQ"
     gene            28119..28409
                     /gene="lsrG"
                     /locus_tag="PN977_001014"
     CDS             28119..28409
                     /gene="lsrG"
                     /locus_tag="PN977_001014"
                     /EC_number="5.3.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(4S)-4-hydroxy-5-phosphonooxypentane-2,3-dione
                     isomerase"
                     /protein_id="HDI6041074.1"
                     /translation="MHVTLVEINVHEDKVDEFIEVFRQNHLGSVQEEGNLRFDVLQDP
                     EVNSRFYIYEAYKDEDAVAFHKTTPHYKTCVAKLESLMTGPRKKRLFNGLMP"
     gene            28466..29224
                     /gene="tam"
                     /locus_tag="PN977_001015"
     CDS             28466..29224
                     /gene="tam"
                     /locus_tag="PN977_001015"
                     /EC_number="2.1.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trans-aconitate 2-methyltransferase"
                     /protein_id="HDI6041075.1"
                     /translation="MSDWNPSLYLHFAAERSRPAVELLARVPLENIKYVADLGCGPGN
                     STALLHQRWPAARITGVDSSPAMIAEARSALPDCQFVEADIRNWQPEQALDLIFANAS
                     LQWLPDHYELFPHLVSLLNSHGVLAVQMPDNWLEPTHVLMREVAWEQNYPDRGREPLA
                     GVHAYYDILSEAGCEVDIWRTTYYHQMPSHQAIIDWVTATGLRPWLQDLTESEQQHFL
                     TRYHQMLEEQYPLQENGEILLAFPRLFIVARRTE"
     gene            complement(29228..30142)
                     /locus_tag="PN977_001016"
     CDS             complement(29228..30142)
                     /locus_tag="PN977_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bestrophin family inner membrane protein"
                     /protein_id="HDI6041076.1"
                     /translation="MIVRPQQHWLRRIFVWHGSVLSKISSRLLLNFLFSIAVIFMLPW
                     YTHLGIKFTLAPFSILGVAIAIFLGFRNNAGYARYVEARKLWGQLMIASRSLLREVKT
                     TLPDSASVREFARLQIAFAHCLRMTLRKQPQAEVLAHYLKTEDLQRVLASNSPANRIL
                     LIMGEWLAVQRRNGQLSDILFISLNDRLNDISAVLAGCERIAYTPIPFAYTLILHRTV
                     YLFCIMLPFALVVDLHYMTPFISVLISYTFISLDCLAEELEDPFGTENNDLPLDAICN
                     AIEIDLLQMNDEAEIPAKILPDRHYQLT"
     gene            complement(30339..31790)
                     /gene="uxaB"
                     /locus_tag="PN977_001017"
     CDS             complement(30339..31790)
                     /gene="uxaB"
                     /locus_tag="PN977_001017"
                     /EC_number="1.1.1.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HDI6041077.1"
                     /translation="MKTLNRRDFPGAQYPERIIQFGEGNFLRAFVDWQIDLLNEHTDL
                     NSGVVVVRPIETSFPPSLSTQDGLYTTIIRGLNEKGEAVSDARLIRSVNREISVYSEY
                     DEFLKLAHNPEMRFVFSNTTEAGISYHAGDKFDDAPAVSYPAKLTRLLFERFSHFNGA
                     LDKGWIIIPCELIDYNGDALRELVLRYAQEWALPEAFIQWLDQANSFCSTLVDRIVTG
                     YPRDEVAKLEEELGYHDGFLDTAEHFYLFVIQGPKSLATELRLDKYPLNVLIVDDIKP
                     YKERKVAILNGAHTALVPVAFQAGLDTVGEAMNDAEICAFVEKAIYEEIIPVLDLPRD
                     ELESFASAVTGRFRNPYIKHQLLSIALNGMTKFRTRILPQLLAGQKANGTLPARLTFA
                     LAALIAFYRGERNGETYPVQDDAHWLERYQQLWSQHRDRVIGTQELVAIVLAEKDHWE
                     QDLTQVPGLVEQVANDLDAILEKGMREAVRPLC"
     gene            complement(32018..33436)
                     /locus_tag="PN977_001018"
     CDS             complement(32018..33436)
                     /locus_tag="PN977_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HDI6041078.1"
                     /translation="MHVQPISTFRLFQEGHLLRNSIAIFVLTTLFYFIGAELRLVHEL
                     SLFWPLNGVMAGVFARYVWLNRLHYYAISYVAMLVYDAITTEWGLVSLAINFSNMMFI
                     VTVALLVARDKRLGKNKYEPVSALRLFNYCLIAALLCAIVGAIGSVSIDSLDFWPLLA
                     DWFSEQFSTGVLIVPCMLTLAIPGVLPRFKAEQMMPAIALIVSVIASVVIGGAGSLAF
                     PLPALIWCAVRYTPQVTCLLTFVTGAVEIVLVANSVIDISVGSPFSIPEMFSARLGIA
                     TMAICPIMVSFSVAAINSLMKQVALRADFDFLTQVYSRSGLYEALKSPSLKQTQHLTV
                     MLLDIDYFKSINDNYGHECGDKVLSVFAQHIQKIVGDKGLVARMGGEEFAVAVPSVNP
                     VDGLLMAEKIRKGVELQPFTWQQKTLYLTVSIGVGSGCASYRTLTDDFNKLMVEADTC
                     LYRSKKDGRNRTSTMRYGEEVV"
     gene            complement(33575..33934)
                     /locus_tag="PN977_001019"
     CDS             complement(33575..33934)
                     /locus_tag="PN977_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4186 domain-containing protein"
                     /protein_id="HDI6041079.1"
                     /translation="MQSLDPLFARLSRSKFRSRFRLGVKERQYCLEKGAPVIEQHAAD
                     FVAKRLAPALPANDGKQTPMRGHPVFIAQHATATCCRGCLAKWHNIPQGVSLSEEQQR
                     YIVAVIYHWLVIQMNQP"
     gene            complement(33934..34860)
                     /gene="glsB"
                     /locus_tag="PN977_001020"
     CDS             complement(33934..34860)
                     /gene="glsB"
                     /locus_tag="PN977_001020"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase B"
                     /protein_id="HDI6041080.1"
                     /translation="MAVAMDNAILENILRQVRPLIGQGKVADYIPALATVDGSRLGIA
                     ICTVDGQLFQAGDAQERFSIQSISKVLSLVVAMRHYSEEEIWQRVGKDPSGSPFNSLV
                     QLEMEQGIPRNPFINAGALVVCDMLQGRLSAPRQRMLEVVRGLSGVSDISYDTVVARS
                     EFEHSARNAAIAWLMKSFGNFHHDVTTVLQNYFHYCALKMSCVELARTFVFLANQGKA
                     IHIDEPVVTPMQARQINALMATSGMYQNAGEFAWRVGLPAKSGVGGGIVAIVPHEMAI
                     AVWSPELDDAGNSLAGIAVLEQLTKQLGRSVY"
     gene            complement(34924..36312)
                     /gene="sad"
                     /locus_tag="PN977_001021"
     CDS             complement(34924..36312)
                     /gene="sad"
                     /locus_tag="PN977_001021"
                     /EC_number="1.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate-semialdehyde dehydrogenase"
                     /protein_id="HDI6041081.1"
                     /translation="MTITPATHAISINPATGEQLSVLPWAGADDIENALQLAAAGFRD
                     WRETNIDYRAEKLRDIGKALRVRSEEMAQMITREMGKPINQARAEVAKSANLCDWYAE
                     HGPAMLKAEPTLVENQQAVIEYRPLGTILAIMPWNFPLWQVMRGAVPIILAGNGYLLK
                     HAPNVMGCAQLIAQVFKDAGIPQGVYGWLNADNDGVSQMIKDSRIAAVTVTGSVRAGA
                     AIGAQAGAALKKCVLELGGSDPFIVLNDADLELAVKAAVAGRYQNTGQVCAAAKRFII
                     EEGIASAFTERFVAAAAALKMGDPRDEENTLGPMARFDLRDELHHQVEKTLAQGARLL
                     LGGEKMAGAGNYYPPTVLANVTPEMTAFREEMFGPVAAITIAKDAEHALELANNSEFG
                     LSATIFTTDETRARQMAARLECGGVFINGYCASDARVAFGGVKKSGFGRELSHFGLHE
                     FCNIQTVWKDRV"
     gene            36413..37294
                     /locus_tag="PN977_001022"
     CDS             36413..37294
                     /locus_tag="PN977_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDI6041082.1"
                     /translation="MDLTQLEMFNAVAEAGSITQAAAKVHRVPSNLTTRLRQLETELG
                     IDLFIRENQRLRLSPAGHNFLRYSQQILALVDEARSVVAGDEPQGLFSLGSLESTAAV
                     RIPVTLAEFNRRYPKIQFSLSTGPSGTMLEGVLEGKLNAAFIDGPINHTAIDGIPVYR
                     EELMIVTPQGHAPVTRASQVNGSNIYAFRANCSYRRHFESWFHADGAAPGTIHEMESY
                     HGMLACVVAGAGIALIPRSMLESMPGHHQVEAWPLAEQWRWLTTWLVWRRGAKTRPLE
                     AFIQLLDVPDSAKQGYQ"
     gene            37372..38487
                     /gene="yneK"
                     /locus_tag="PN977_001023"
     CDS             37372..38487
                     /gene="yneK"
                     /locus_tag="PN977_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative protein YneK"
                     /protein_id="HDI6041083.1"
                     /translation="MVTPVSISNYISLPDDFPVRNIAPQVKEVLKDFIDALSTIICDE
                     EWRTSLNINSATKKIFNNLDNLSYIQRTSFRGNDTLYNEKVQFKLTYPVKNGRHKENI
                     EFQVVINLSPIYLDNFRHDGEINIFCAPNPKPVTMGRVFQTGVERVLFLFLNDFIEQF
                     PIINPGVPIKRAHTPHIEPLHPDHHTAADYIRQFDLLVLNFISRGNFVILPRLWNNSE
                     VHRWFGNKEPNLLTAILDITDSELKEDLLQSLMDSLGSNKHVLPEVCICFLSLLAEQD
                     FPHFQELFLFFANMLLHYHQFMNPNESDLIDVLMPASLSDDKIIKHMARKTLKLFVKN
                     ETPPKVTHEDLVKNRPRSPVRPPIPATAKTPDLPERH"
     gene            38637..39827
                     /gene="ydeA"
                     /locus_tag="PN977_001024"
     CDS             38637..39827
                     /gene="ydeA"
                     /locus_tag="PN977_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose MFS transporter"
                     /protein_id="HDI6041084.1"
                     /translation="MTTNTVSRKVAWLRVVTLAVAAFIFNTTEFVPVGLLSDIAQSFH
                     MQTAQVGIMLTIYAWVVALMSLPFMLMTSQVERRKLLICLFVVFIASHVLSFLSWSFT
                     VLVISRIGVAFAHAIFWSITASLAIRMAPAGKRAQALSLIATGTALAMVLGLPLGRIV
                     GQYFGWRMTFFAIGIGALITLLCLIKLLPLLPSEHSGSLKSLPLLFRRPALMSIYLLT
                     VVVVTAHYTAYSYIEPFVQNIAGFSANFATALLLLLGGAGIIGSVIFGKLGNQYASTL
                     VSTAIALLLVCLALLLPAANSEIHLGVLSIFWGIAMMIIGLGMQVKVLALAPDATDVA
                     MALFSGIFNIGIGAGALVGNQVSLHWSMSMIGYVGAVPAFAALIWSIIIFRRWPVTLE
                     EQTQ"
     gene            complement(39852..40517)
                     /gene="marC"
                     /locus_tag="PN977_001025"
     CDS             complement(39852..40517)
                     /gene="marC"
                     /locus_tag="PN977_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter MarC"
                     /protein_id="HDI6041085.1"
                     /translation="MLDLFKAIGLGLVVLLPLANPLTTVALFLGLAGNMNSAERNRQS
                     LMASVYVFAIMMVAYYAGQLVMDTFGISIPGLRIAGGLIVAFIGFRMLFPQQKAIDSP
                     EAKSKSKELEDEPSANIAFVPLAMPSTAGPGTIAMIISSASTVRQSSTFADWVLMVAP
                     PLIFFLVAVILWGSLRSSGAIMRLVGKGGIEAISRLMGFLLVCMGVQFIINGILEIIK
                     TYH"
     gene            40729..41163
                     /gene="marR"
                     /locus_tag="PN977_001026"
     CDS             40729..41163
                     /gene="marR"
                     /locus_tag="PN977_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance transcriptional
                     regulator MarR"
                     /protein_id="HDI6041086.1"
                     /translation="MKSTSDLFNEIIPLGRLIHMVNQKKDRLLNEYLSPLDITAAQFK
                     VLCSIRCAACITPVELKKVLSVDLGALTRMLDRLVCKGWVERLPNPNDKRGVLVKLTT
                     SGAAICEQCHQLVGQDLHQELTKNLTADEVATLEHLLKKVLP"
     gene            41184..41567
                     /gene="marA"
                     /locus_tag="PN977_001027"
     CDS             41184..41567
                     /gene="marA"
                     /locus_tag="PN977_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     MarA"
                     /protein_id="HDI6041087.1"
                     /translation="MSRRNTDAITIHSILDWIEDNLESPLSLEKVSERSGYSKWHLQR
                     MFKKETGHSLGQYIRSRKMTEIAQKLKESNEPILYLAERYGFESQQTLTRTFKNYFDV
                     PPHKYRMTNMQGESRFLHPLNHYNS"
     gene            41599..41817
                     /gene="marB"
                     /locus_tag="PN977_001028"
     CDS             41599..41817
                     /gene="marB"
                     /locus_tag="PN977_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance protein MarB"
                     /protein_id="HDI6041088.1"
                     /translation="MKPLLSAIAAALILFSTQGIAEQTTQPVVTSCANVVVVPPSQEQ
                     PPFDLNHMGTGSDKSDALGVPYYNQHAM"
     gene            complement(41874..43313)
                     /locus_tag="PN977_001029"
     CDS             complement(41874..43313)
                     /locus_tag="PN977_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000012594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HDI6041089.1"
                     /translation="MSGFKKGFLWGGAVAAHQLEGGWNEGGKGISIADVMTAGAHGVP
                     REVTEGIIDGLNYPNHEAIDFYHRYKTDIQLFAEMGFKCFRTSIAWTRIFPQGDEQEP
                     NEEGLQFYDDLFDECLKQGMEPVVTLSHFEMPYHLVTKYGGWRHRKLIDFFIRFASTV
                     FTRYKEKVKYWMTFNEINNQVNFSESLCPFTNSGILYSPEEDINEREQIMYQAVHYEL
                     VASALAVQTGKSINPEFNIGCMIAMCPIYPLTCAPNDMMMATKAMHRRYWFTDVHARG
                     YYPQHMLNYFARKGFNLDITPEDNTILARGCVDFIGFSYYMSFTTQFSPDNPQLNYVE
                     PRDLVSNPYIDTSEWGWQIDPAGLRYSLNWFWDHFQLPLFIVENGLGAVDQRQADGTV
                     NDHYRIEYFASHIREMKKAVVEDGVDLIGYTPWGCIDLVSAGTGEMKKRYGMIYVDKD
                     NEGKGTLERIRKASFYWYRDLIANNGENI"
     gene            complement(43338..45012)
                     /locus_tag="PN977_001030"
                     /pseudo
     CDS             complement(43338..45012)
                     /locus_tag="PN977_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233185.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate porin"
     gene            complement(45068..45379)
                     /locus_tag="PN977_001031"
     CDS             complement(45068..45379)
                     /locus_tag="PN977_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001314746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HDI6041090.1"
                     /translation="MFADEELVMELLVNAGQARSDAMEAIRCAGQKDWQGATQLMASS
                     ESACLQAHKIQTALISQDEGCGKIKVNLILVHAQDHLMNAILCQDLAREIISLRKELH
                     A"
     gene            complement(45407..46729)
                     /locus_tag="PN977_001032"
     CDS             complement(45407..46729)
                     /locus_tag="PN977_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001736301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDI6041091.1"
                     /translation="MGLMASFERGMERFLVPVAIKLNSQKHVAAVRDGFVFTFPIIMA
                     SSLIILINFAILSPDGFIAGLLHLNSVFPNLEKAQAIFTPVMNGSVNIMSIMIAFLVA
                     RNMAISYEQDDLLCGLTAIGAFFIVYTPYQMIDGQAFLTTKYLGAQGLFVAVIVALIT
                     SEIFCRLARNPKITITMPAAVPPAVARSFKVLLPIFFVMVFFSALNYCLTLISPAGLN
                     DLIYTLIQTPLKHMGTNIFAVIILGAVGNFLWVLGIHGPNTTSAIRETVFSEANLENL
                     SWAAQHGTTWGAPYPITWTSINDAFANCGGSGMTLGLLLAIFIASKRAEYRDLAKMSF
                     IPGIFNINEPIMFGLPIVLNPIMMVPFIMVPIVNCAIGYFFVSMEIIPPVAYAVPWTT
                     PGPLIAFLGTGGNWLALLVGFLCLGVATMIYLPFVIAANKVNNLTTNG"
     gene            complement(46843..47154)
                     /locus_tag="PN977_001033"
     CDS             complement(46843..47154)
                     /locus_tag="PN977_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000722572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDI6041092.1"
                     /translation="MKKILLVCAAGMSTSMLVKRMIDHANAISLEVNISALAIAEAKG
                     KIKNNEVDVVLLGPQVRFQKPEIEAVAQGKMPVAVIEMKDYGTMNGQAVLEFAMKLLQ
                     Q"
     gene            47353..48051
                     /locus_tag="PN977_001034"
     CDS             47353..48051
                     /locus_tag="PN977_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000577178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDI6041093.1"
                     /translation="MIFQKIARLLKSEINGYSWHVGDLLPSEAELAVRYNVSRNTLRK
                     ALSLLEGEGIIHRKHGSGTYIQKKNFVAHIDHMNSFSEIAHKSGKEAGSQIMKFEVQD
                     ASPPIATELNLVTGEQVYYIKRLRFIEDNAAQLEETWMSVARFPDLTVAHMQKSKFSY
                     IENECGIKIIGTFETFSPTFPTPEIASILRISPRDPILKIQTQAVDSNSIPLDYSLLY
                     SNIFEFQVKYFFPR"
     gene            complement(48096..48995)
                     /gene="eamA"
                     /locus_tag="PN977_001035"
     CDS             complement(48096..48995)
                     /gene="eamA"
                     /locus_tag="PN977_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylserine/cysteine exporter"
                     /protein_id="HDI6041094.1"
                     /translation="MSRKDGVLALLVVVVWGLNFVVIKVGLHNMPPLMLAGLRFMLVA
                     FPAIFFVARPKVPLNLLLGYGLTISFAQFAFLFCAINFGMPAGLASLVLQAQAFFTIV
                     LGAFTFGERLHGKQLAGIALAIFGVLVLIEDSLNGQHVAMLGFMLTLAAAFSWACGNI
                     FNKKIMSHATRPAVMSLVIWSALIPIIPFFVASLILDGSATMIHSLVTIDMTTILSLM
                     YLAFVATIVGYGIWGTLLGRYETWRVAPLSLLVPVVGLASAALLLDERLTGLQFFGAV
                     LIMTGLYINVFGLRWRKAVKVRG"
     gene            49190..50377
                     /gene="ydeE"
                     /locus_tag="PN977_001036"
     CDS             49190..50377
                     /gene="ydeE"
                     /locus_tag="PN977_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux MFS transporter YdeE"
                     /protein_id="HDI6041095.1"
                     /translation="MNLSLRRSTSALLASSLLLTIGRGATLPFMTIYLSRQYSLSVDL
                     IGYAMTIALTIGVVFSLGFGILADKFDKKRYMLLAITAFASGFIAIPLVNNVTLVVLF
                     FALINCAYSVFATVLKAWFADNLSSTSKTKIFSINYTMLNIGWTIGPPLGTLLVMQSI
                     NLPFWLAAICSAFPMLFIQIWVKRSEKIIATETGRAWSPKVLLQDKALLWFTCSGFLA
                     SFVSGAFASCISQYVMVIADGDFAEKVVAVVLPVNAAMVVTLQYSVGRRLNPANIRAL
                     MTAGTLCFVIGLVGFIFSGNSLLMWGMSAAVFTVGEIIYAPGEYMLIDHIAPPGMKAS
                     YFSAQSLGWLGAAINPLVSGIVLTSLPPFSLFIILALVIVAAWVLMLKGIRARPWGQP
                     ALC"
     gene            50504..50599
                     /gene="mgtS"
                     /locus_tag="PN977_001037"
     CDS             50504..50599
                     /gene="mgtS"
                     /locus_tag="PN977_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein MgtS"
                     /protein_id="HDI6041096.1"
                     /translation="MLGNMNVFMAVLGIILFSGFLAAYFSHKWDD"
     gene            complement(50818..51708)
                     /gene="dgcZ"
                     /locus_tag="PN977_001038"
     CDS             complement(50818..51708)
                     /gene="dgcZ"
                     /locus_tag="PN977_001038"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcZ"
                     /protein_id="HDI6041097.1"
                     /translation="MIKKTTEIDAILLNLNKAIDAHYQWLVSMFHSVVARDASKPEIT
                     DNHSYGLCQFGRWIDHLGPLDNDELPYVRLMDSAHQHMHNCGRELMLAIVENHWQDAH
                     FDAFQEGLLSFTAALTDYKIYLLTIRSNMDVLTGLPGRRVLDESFDHQLRNAEPLNLY
                     LMLLDIDRFKLVNDTYGHLIGDVVLRTLATYLASWTRDYETVYRYGGEEFIIIVKADN
                     DEEACRAGIRICQLVDNHAITHSEGHINITVTAGVSRAFPEEPLDVVIGRADRAMYEG
                     KQTGRNRCMFIDEQNVIHRV"
     gene            complement(51963..52355)
                     /gene="ydeI"
                     /locus_tag="PN977_001039"
     CDS             complement(51963..52355)
                     /gene="ydeI"
                     /locus_tag="PN977_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI family stress tolerance OB fold protein"
                     /protein_id="HDI6041098.1"
                     /translation="MKFQAIVLASFLVMPYALADDQGGLKQDAAPPPPHAIEDGYRGT
                     DDAKKMTVDFAKTMHDGASVSLRGNLISHKGEDRYVFRDKSGEINVVIPAAVFDGREV
                     QPDQMINISGSLDKKSAPAVVRVTHLQK"
     gene            complement(52718..54763)
                     /gene="dcp"
                     /locus_tag="PN977_001040"
     CDS             complement(52718..54763)
                     /gene="dcp"
                     /locus_tag="PN977_001040"
                     /EC_number="3.4.15.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-dipeptidase Dcp"
                     /protein_id="HDI6041099.1"
                     /translation="MTTMNPFLVQSTLPYLAPHFDQIANHHYRPAFDEGMQQKRAEIA
                     AIALNPQTPDFNNTILALEQSGELLTRVTSVFFAMTAAHTNDELQRLDEQFSAELAEL
                     ANDIYLNGELFARVDAVWQRRESLGLDSESIRLVEVIHQRFVLAGAKLKQADKAKLKV
                     LNTEAATLTSQFNQRLLAANKSGGLVVNDIAQLAGMSEQEIVLAAEAAREKGLYNKWL
                     IPLLNTTQQPALAELCDRATREKLFTAGWMRAEKNDANDTRAIIQRLVEIRAQQAKLL
                     GFPHYAAWKIADQMAKTPEAALNFMREIVPAARQRASDELASIQAIIDKQQGGFSAQP
                     WDWAFYAEQVRREKFDLDESQLKPYFELNTVLNEGVFWTANQLFGIKFVERFDIPVYH
                     PDVRVWEIFDHNGVGLALFYGDFFARDSKSGGAWMGNFVEQSTLNETHPVIYNVCNYQ
                     KPAAGEPALLLWDDVITLFHEFGHTLHGLFARQRYATLSGTNTPRDFVEFPSQINEHW
                     ATHPQVFARYARHYQSGAAMPDELQQKMRNASLFNKGYEMSELLSAALLDMRWHCLEE
                     NEAMQDVDDFELRALVAENMDLPAIPPRYRSSYFAHIFGGGYAAGYYAYLWTQMLADD
                     GYQWFVEQGGLTRENGLRFREAILSRGNSEDLERLYRQWRGKAPQIMPMLQHRGLNV"
     gene            54900..55646
                     /gene="ydfG"
                     /locus_tag="PN977_001041"
     CDS             54900..55646
                     /gene="ydfG"
                     /locus_tag="PN977_001041"
                     /EC_number="1.1.1.298"
                     /EC_number="1.1.1.381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional NADP-dependent 3-hydroxy acid
                     dehydrogenase/3-hydroxypropionate dehydrogenase YdfG"
                     /protein_id="HDI6041100.1"
                     /translation="MIVLVTGATAGFGECITRRFIQQGHKVIATGRRQERLQELKDEL
                     GDNLYIAQLDVRNRAAIEEMLASLPAEWCNIDILVNNAGLALGMEPAHKASVEDWETM
                     IDTNNKGLVYMTRAVLPGMVERNYGHIINIGSTAGSWPYAGGNVYGATKAFVRQFSLN
                     LRTDLHGTAVRVTDIEPGLVGGTEFSNVRFKGDDGKAEKTYQNTVALTPEDVSEAVWW
                     VSTLPAHVNINTLEMMPVTQSYAGLNVHRQ"
     gene            55735..56421
                     /gene="ydfH"
                     /locus_tag="PN977_001042"
     CDS             55735..56421
                     /gene="ydfH"
                     /locus_tag="PN977_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YdfH"
                     /protein_id="HDI6041101.1"
                     /translation="MTVETQLNPTQPVNQQIYRILRRDIVHCLIAPGTPLSEKEVSVR
                     FNVSRQPVREAFIKLAENGLIQIRPQRGSYVNKISMAQVRNGSFIRQAIECAVARRAA
                     SMITESQCYQLEQNLHQQRIAIERKQLDDFFELDDNFHQLLTQIADCQLAWDTIENLK
                     ATVDRVRYMSFDHVSPPEMLLRQHLEIFSALQKRDGDAVERAMTQHLQEISESVRQIR
                     QENSDWFSEE"
     gene            56598..56801
                     /gene="ydfZ"
                     /locus_tag="PN977_001043"
     CDS             56598..56801
                     /gene="ydfZ"
                     /locus_tag="PN977_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenium delivery protein YdfZ"
                     /protein_id="HDI6041102.1"
                     /translation="MTTYDRNRNAITTGSRVMVSGTGHTGKILSIDTEGLTAEQIRRG
                     KTVVVEGCEEKLAPLDLIRLGMN"
     gene            complement(56837..>57730)
                     /locus_tag="PN977_001044"
     CDS             complement(56837..>57730)
                     /locus_tag="PN977_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HDI6041103.1"
                     /translation="HVMRDVVTSYAQAVDVKLAQWIEENVTFPSTMVDRIVPAVTEDT
                     LAKIEQLTGVRDPAGVACEPFRQWVIEDNFVAGRPEWEKAGAELVSDVLPYEEMKLRM
                     LNGSHSFLAYLGYLAGYQHINDCMEDEHYRHAAYALMLQEQAPTLKVQGVDLQDYANR
                     LIERYSNPALRHRTWQIAMDGSQKLPQRMLDSVRWHLAHDSKFDLLALGVAGWMRYVG
                     GVDEQGNPIEISDPLLPVIQKAVQSSAEGKARVQSLLAIKAIFGDDLPDNSLFTAKVT
                     EAYLSLLAHGAKATVAKYSVK"
BASE COUNT        15183 a        14075 c        13048 g        15424 t
ORIGIN      
        1 ctttcaaact gacggatgaa aatttcaccc acatcgtttt taacccattg cataaaaatg
       61 gttgacataa acttgccata agccgtagga ggcacagaaa aatcaatgat acggaatgtg
      121 ttctcactat gaccactgaa atcaatattc ggcgtcccgg tttcgagcaa ttcgataaat
      181 tgcatatgtt tactgccgat agattttaaa aaatgataaa cctcaagagg gtaatggaca
      241 ttaacgttat taatgacggt taacgtatta aactctactt gatatgattt cagacgctcg
      301 atggctgcta tcacttttgc aaaagtaccg ttacctgaat tactgcgtct gtaacggtca
      361 tgtagctcct ggggtccatc gatcgagata ccaaccagaa actcatgttc tttgagaaag
      421 gcacaccatt cattattcaa taaaatgcca ttcgtttgta atgcattaaa aatacgtttt
      481 tggcctgcat agcgttgttg atagtgaata actttacgga aaaaatccag gccagccaga
      541 gtgggttcac cgccttgcca ggtaaaatag acctgattgc cagacgctgc gatatattgt
      601 ttgatgaact ctttcagagt gctgtcatcc atccattttt catgagtaaa ctgcgactct
      661 ttttcaaggt aaaaacagta atcacatttg agattacatt gaaaactgga gggcttggct
      721 gtaacgtgca tcgctatctc gctcaataag gcggcggaaa aatccgccgc atgaaggttt
      781 agttatttcg cttcgcttag tgctttcttg atattgttaa acttctcctg atttacctcg
      841 ctaagtggtg gttggctgct gtcgataaac tctcttacca cgccttgcat ctctttaacg
      901 acctgcggat tggcggcggc aaggttatct ttttgctgta gatccgtcag tttgtataga
      961 cctaactgat tgttttctac tgtatagaca agcgaataat cgttatttct taccgtataa
     1021 gagaattggc ttaaatcctc ggtgttgggg ttatgcgggt aatcgtctga ctgatggcgg
     1081 acaaatttgt ggtaattatc ccagaatgga atattttcct cgtcaaacca gtgagaataa
     1141 gaggttatcc aggtcagatt tttatgtggc tcgccttgtt tcttatcttg caaccacggc
     1201 agcaaggaaa cgccatccag cttaaggtct tttggaatgc tgatatcggc tgcatcaaga
     1261 gctgtcgggt agaaatccat tgcggaaatc agcttgtcat aattaccggg ttgaagtttt
     1321 cctttccacc acataaacat tggggtgtga gtaccgccag gataggtctg actcttatag
     1381 cctttttgcg ccccgttcag cggcagagga ccatcgataa ccgcaccatt atcggaggta
     1441 aagagaataa tagtattgtc atactgtccg tttttcttca gttgttcgag aatgcgtttt
     1501 acaccctgat caacagaata aacggaagcg tagtagttat ctgctgtttg actaccggta
     1561 ttaaattgct tctgatattg ctccggtgca ggattatcat ttggcaggtg cggagcatta
     1621 taagccaggt aaagcataaa aggctggtca agtgttttgg cacgatcaac aacgccaatt
     1681 gcctcatcgg ttaactgatc gctgatataa ccttttgcgg ggacgcgttc acggtttttg
     1741 aacagtgaag gggagttgta atatgccgtt cctgcagcgt ggaatcccat aaagtaatca
     1801 aagccacggt tttgaggttg ccattcttcc gcagaaaatg tggtgaagtt gtcatgatag
     1861 tcacgcgttt gtttatcttc cggtaccggc acattactga tttttgacaa gtgccattta
     1921 cctactgctg cagtgtaata accatgattc tggaataatt caggcaagaa agtttctgtt
     1981 agcggaatac catcctgagc atcggtattg gaatagacac caaagcgggc gggcgctcga
     2041 ccggtcatta ttgcagcgcg ggaggggccg gaaacaccgt gcgccacata gccgttagta
     2101 aaacgcacgc cttcatccat taatgaaagg agcgtcggcg ttgatttttg cgcagcttca
     2161 atggctttat ctatccctat tttgtaggta tcgacaactt cacgattttc cattgttttt
     2221 gggtcaaaag atcccttatc aaaaggaagt tgtccataac caagatcatc catggtcagt
     2281 acgataatat ttggctttcc tttggtactg tattcagtcg gcgtaaagtc tgagaaagca
     2341 acgtttgttt tggttgcttt cagctttaca tcatctgccg catgagcagc aaatgcagcc
     2401 ataccagatg ccagtatcaa agatatcgag gtacttacga cacttttctt taatgcagac
     2461 ttcataaatg ttcctcttct tattgtactt aattccaaag taattttacg aagagaaata
     2521 agtggatgta agtgaagtga gtcacataaa gagatagcag atttagctaa aaaagggaaa
     2581 taacagtcca taaagcgttg acattacttt ctgttctact aagtaatttc tcgccgataa
     2641 acaactaatc tattgatatt taataaatta ttgcatttta atgacaaaat gccgaattga
     2701 gatcataaat aatcatgcaa caggttatgc aaatgcataa atatgtgatg aatgtcactt
     2761 atttatttca agaaatatat cgcctaaaaa caacgcgggg caggaaatgg ctgccccatt
     2821 taattcttac gtagcgtgtg tggttgacta ctcgttagca aataatcaaa tagctaaagc
     2881 actcatcgtg ttgtccgtgt tcataccggt gtgcctttga caacggtact ccttcgaaac
     2941 tctcttcgga ctgttgccga aatgtttgcg gaacgcataa ataaaataag atgtactcgc
     3001 ataaccacat tgttcggcaa ttttattgac tgaaccttct acgcgtatca aattttttgc
     3061 gtgctgcatt ctggcatcta aaagaatctg tgagaatgtc gtttgctctt gctttagttt
     3121 tttcttcaac aggctttcac tgatgtacag gcagtcacaa atatctttca gcttccatgg
     3181 gtgcgccagc ttcatgttga caatatttct cactttcccg gaaacggata gcacaccgtt
     3241 agttaatagt gtaatgaaac ctttgcatgc ggcaaaaata gacaggcaag aaaaaagcaa
     3301 aagttctgaa aagttatgat gattgcgttc ttccgagttg agataagcaa tcatttcatt
     3361 aagcaatccc gtcggtacat ttgaagcacg taaaaacttc gcaggaacag gcaccgtcac
     3421 tgtctggatg ttagtgcatt gtagatattt tttaatggtg ttttcattaa atccgatttc
     3481 tttgaaatgc cgctcataag gttcaaaatg cgtacgaaca gcgttatcta ccatcaggat
     3541 ctcgccgtca gagaaggcgt aatctttatc taaaatgtta gcgttgaagg catgatgaat
     3601 aaatataaca gaacaaacga gcgacatttt atctccttaa aacaataaag tttttatcga
     3661 tactttttta acatttcaca tttataattt gctgtttatt ttcagtcttg caaactattg
     3721 ataatgaaat gtgtaagatc tcttgccatg cgacccattt ctgaaatgcc ctgctgatca
     3781 attgccgaaa aaatggctat caacgtaagt gtcagcaaca agacaaccac aattacagac
     3841 atgatttctt tatagttgtc tctttgcatg attttgtttt tcactgtggt cgcatgcatt
     3901 tcaaatgtgt ttatttagcg gataacgtta aaaatcgtta atcagtatga ttaatataac
     3961 aggctggata aaagaggaaa taggactggc acttcagtac tgagcggagt ttcttacaac
     4021 tgtaggcaga ggtttatatc ttatagtgtg tagtttaatt taatccatgt gagcaaattg
     4081 cgcgataata gtcaatgagg aattcttctg ctgtgcggat gacagcagaa gaaatgagga
     4141 ggcgaattaa tttgatggct ctaattcaac aggaatactt ttatagccag gaatgccact
     4201 taatggatcg tggttatcaa gtgttagcat gtgattcgat tctggaaaat aggtcaccag
     4261 tgagcggtca gccatagggt aaatgaccac ttttaatcta tccatgcgtc gtgagctacg
     4321 tttaccgtct ggcgtgagtg caataagatt aactctttcg ccgtttttta cacggcaaat
     4381 tttagcttgt ttagcactca taaagaccac atctcgttga ccgaatacac ctcgatagcg
     4441 atcatccata ccataaatcg tcgtgttgta ctgatcgtgg ctgcgtactg tcgccatgac
     4501 cagtttactg ttaaacgctg aagagggatc ttctaacagc cctttgctgg taatgaaatt
     4561 agccttacct gacggcgtca tccagcgcct ttcagcagct gcgtttatca ggtgaaaacc
     4621 accaggatga cggatgcgct ggttatagtc ggcaaactct ggcagcacag cttcaatgtc
     4681 attgcgaatg cgatcataat cttccaccag atactcccag gctaccacgc tctggggtag
     4741 tgctgcctgc gcgattcccg cgaccactgc acactctgat ttcagcatta caccggcggg
     4801 ttttaacaca ccacgcgagg catgaatcat cgacattgaa tcttcaacgg ttaccgcctg
     4861 cgcaccgctt ttttgcatgt caatctcgct acgtcccaga accggcagaa tatagctatg
     4921 ccgtgcggtc agcagatgag agcggttaag cttagtggct acgtgtaccg ccaaatctaa
     4981 ttgcgttaac ggtacagcgc tcgcttcccg atctggcatt gccagcgcaa agttgccccc
     5041 catgcagatc aatgctcgag cttgccccgt acatattgct tgcatgctgg caattgcagc
     5101 atgtccaggt gcatgaggtg gggtgaagcc atagcgctca cccaggcgat ccagaaactc
     5161 tgcagacggt ttctcggtga taccgacggt tcggtcgccc tgtacattag agtgacctcg
     5221 tagtgggcag atacccgcac caggcttgcc aatgttacct ttcatcaaca gcagattgac
     5281 cagttgctgt acgttctggg taccatgttc gtgctgagtg atccccattc cgtaacagat
     5341 aatggttcgt tcggcagcgg catatgcatc agcaagttcg gcgatttgtg tctgacttag
     5401 tccagatata cgttcgatat ctttccactc ggaattgagc acgtcacggc gtagctcgtc
     5461 aaagccgacg gtatgcgttt gaataaattc atcatccagc aatgagggcc gacctgcggc
     5521 gcttgcagca tcatcgcgct caattaacag gcgcatcatt cccttgagca acgccatatc
     5581 accaccaatg cgcacgttgt agtaggcact ggccaactga gtctcagagt tcgtcagcat
     5641 ttcaaacggg ttttgcggtg cggtaaatcg ctccaggcca cgttcctgta gaggattgat
     5701 ggcgatcatt ttcgctcccc gtttcactaa agcgcgcaac gaagtcaaca tgcgagggtg
     5761 gtttgtacca gggttatgcc caatgcaaat gactaaatcg catttctcaa agtcttccag
     5821 caacacggtc cctttaccta caccgatact cgctgccaaa cccacactgg tcggttcatg
     5881 gcacatgttg gagcagtcgg ggaagttatt gctcccgtat tcacgggcaa aaagctgata
     5941 aagaaaggca gcttcattgg aagtgcggcc cgaagtatag aattcaacct gattgggatc
     6001 actatagctt tgaaggcgtt cgccaatttc gtcgaaagct tgttgccagc ttaatggctt
     6061 gtaacagtcg ctgacggcat catatttcaa aggctgggtg agacgccccg cagcctcaag
     6121 ctcgtgatct ccccaggtaa gtaatgattg aaccgtattc tcagcaaaga aagaggcatt
     6181 tacctgctta tccgtgactt cccaggcgat tgcttttgcg ccgttttcac aaatgtcgaa
     6241 tgacgcactg tgcttaggat ctggccatgc acaacccgga cagtcaaagc cctctggctt
     6301 attcatgtca aacatggcaa taacatcctg gcgtatatcc atctgcttac gtactgcatt
     6361 cgctacggat ttaacagcac cccaaccacc ggcagccccc tggtaggatt caattttttt
     6421 cttcatctac ttatcctgtg tcgaaatgaa ttattaggat ttgaaatagc gatgatgcac
     6481 agtatataca aagggattat cgtcaatatg gatatataag agtaggggac tactctcttt
     6541 ctatttttaa tctattatcg ttaaataata gaatggtata aatacgataa gttaagcatc
     6601 atctctcata gtgtttaaat taacatgttt ttcaatataa aaaataagac tctggcgtta
     6661 attgtgcgcg gattttctta caggtgtagg ataatagcct tcacaaaata attatccgcg
     6721 caattttcgc gaatggagcg catagactaa ggtcaaaatc acaattattc atagataaaa
     6781 gtgacaccaa tgactgactg gacggttccg gctgcaacct tgtttcctgt ttgtccatag
     6841 gttgcgctta aacccagagg tactttgctt ttatttactg tccccaggga gacgttttct
     6901 cccgtagcca gtatttttcc attacgcatc agagagacac ccactcccga tgcttttgtt
     6961 gcatctggtg ctgtgttagc aaacacttgt ctggcactgt cagtggtagc acccgaaaga
     7021 taaaatgaga gcttttgttc acttgagcaa tatacgccaa gggggatttc tgcgcttcca
     7081 ggaaagtccg ggagattcac attgacatta cgactatcaa cggtgcagcc gcctgttggc
     7141 ataacaacac tgttatttga aataatattc cacgtaaaat tacgcggatt tccgcttcct
     7201 aacgtggcaa ttttatacat atgaatacgg gcaataactt ccccggcttt tatgacaact
     7261 ccaccggccg caccgactgg ggtaatatag agttttaacg gtaatggcat cggtgtctta
     7321 tcaccaatat ctaatacatt agtatttgtc gtcaacggga aggggtaggt cacattattc
     7381 cagtacagcg atcctttata tgactgtaga gagcctgcga aagctgatcc ttgtaccagg
     7441 tttatatgat cagtgtcgta ccagccgccg taatcattcc agcaacttat atgctgagac
     7501 aagtctacaa ccagattctg gcccggttgt attacagggt cgagattaac ataaacagag
     7561 gttgtacctg ctccaatact tgacccgcca tcgacattac aactgaaggc aaaaactttg
     7621 cctgccatta atagaagata aataccgaac agaagcttca tacttattgt tttacccatg
     7681 atatatccta agattaaaaa attgatttaa aaaggaggct aagaaaatcg aatcgacgtt
     7741 attgccaggt gtagatcaca ttgattagcg cctcgatcgt tccctggctt gcgtttccat
     7801 tcaccgtgat agctcttgcc ttaagtggaa actgtgcatt acgagtgatc tcatcaacaa
     7861 taaccgtttt gctatcacca ttttttaacg tcgcatcctg gtcatccctt agctctatct
     7921 gaatattttc ggcagtacct tcatttttgt aataacccgt attgtcagtt gaacctgtca
     7981 cgattgccgt cactgcactt gtttcaaccg gacaatcggt taatgacaaa gtaatattgt
     8041 gccagcctga tgcagaacca ggttgttgca gattgcgcgt ataaagatcc ccgagattaa
     8101 cgttagcttc tttggtttga atagtgcagg gtttagcgac tacccgacca ataacagtga
     8161 tagtgacatc ggcggcctgg agagttgctg tgaataacgc gcaaaacgta gctaacagga
     8221 acctcttgtg aagtcttttc atatctgact ctgattattg aaattcaagg gtaaaggttg
     8281 ccgaagcgct caccagtcca ggattgacgg acttctgagt tgacttcaaa cgagcggagt
     8341 aaggcaaaat attgttctgt tctggtacca atgggatcca ctgcatcccg gcatgaaggt
     8401 cattcaattt caccggacgc atatttccgt ccagtatttc tatacccaaa ccggaggccg
     8461 tattgcttcc ttcatccagt ttcaacagag tattattttc tgcatcctca ataccgttaa
     8521 atgcaacccg aactcccgtt gtccctttgc tgcattcact taatgtaatc tgaaaaggga
     8581 cggctggact tgtgctaccg gtcgttggaa attgtctggc actgtttttt tggagatcta
     8641 cggtaaaatt aagcgaatcc gatgagactg tgcagccata atcgagaacg cgcccgctaa
     8701 ttttaataac gctatctgcg gataaagcag aataggtggt taaccccaga cataaaccga
     8761 ggaaaataac gttattgtat ttcataatct attgttcctt agcgacagat tgctgtctgc
     8821 tggttcagca aggttccagg agagactgca ggaagcttgt aatcgacaat acagtttgag
     8881 tttttatcat tgccccatga aacctgtaat ttccctgact gtggaagtcc agtcagataa
     8941 acctgaccat tttccgcgac aatgctgcca tttttattct ctccgtgagt gacaattgca
     9001 ccgaatggaa cgcttttatt accgtacttc aacgtcatta atactttccc gccgatttgt
     9061 gcattaaatg ttgctctggc aatagcaccg tgagttggga tgacagtgac tacggtttca
     9121 tccagttcaa cattatctgc aagggaattc gcgttaagag cgacacgatt ttctctatat
     9181 tctgtcgcaa atggtaatat ggcatagcca cgccagtcgg tatgaattcc ggtctggttc
     9241 tctattttga cattatcagc gccaggagcc ttaaccagaa ccattgtgtc gcccagtggc
     9301 tgtccaaagg tgatgccatc agcatgagca ataatgccac cactcattcc gtaataaatc
     9361 tggctgctgt caccactccg actgtaaccg atattcgtat taccgtaagc tccacgataa
     9421 ttaagagaac tgtaaccact ggtgccagac gatgtattac ccccgtgggt gttaccgacc
     9481 tgaacactat aattcaggtt attatccggc agcagagtgc cataaacccc tgataggttg
     9541 gtcatgccgc ctttcaaatc gtttgacata ctgtaactgg cgtttgagtt acgaaatgcc
     9601 gactgactgt ctgtacgcat ccaatgactg aagggaacat taagcgtaaa agcaagtaaa
     9661 tgatcccgat cgttttgcca tatattattg gaatagctgt aattcagcga agtcgtaata
     9721 tcactaaacg gcacatttaa tccaaatgat atttgctggt cgctgcgtga cgtgttccag
     9781 taactttggc gactggcact gaaaaatgtc gtaccgtaat ttccaagctg ctgagagatg
     9841 cttatttgtt cctgaccacg cttactgtag aacagattaa aataatcaat aaattgtgtc
     9901 tgctcattgc tgtctccggt aggaggcttg acggtgtaac cactcattcg actgtaggca
     9961 ctgtcggata agttataaaa accttgcgta gaatagcgat atcctgcgac ctggatattg
    10021 gttcccgtct ggtagaagga tttgctgtag acggatttaa tcgattgccc gctgtgacgg
    10081 gtgccatctg caagtgtcgt attggcctgc gtgatatcga aagaaaaggc accaaaaagt
    10141 cctaaatctt taccaatacc aaggttgaag gcctgataat cttctgcaat ttgcattccg
    10201 ccataaggtg tccagtttcc ttccagtcca tgcatcaagc taccttgtat gaacttgggg
    10261 gagctttgca ggttatttcc actacgatat tcccccatgg caagcgcata acgcgtatat
    10321 ccagcacgtt ggagaaccgg aacagacgaa taaggaacat ataatgtctg aatactgccg
    10381 tctgcctctt ttatggttac ttgcagatca ccaccattgg ccgcagagtt gatatcatca
    10441 atagtaaaag ggccgggtgg gacagtagtc tgataaacat cgtatccatt ctgttttaca
    10501 ctcacttgtg cggtgctgcg ggcaatacca tgaatcaccg gagcgaaacc atgttggcta
    10561 tcgggcaaca tcgcttctgt tgaatttatt ttgaggccac gaaagttcac actatcaaaa
    10621 atatcgccat cggtataact atcacctacc gttaagcgtg agcgtaaggg aataatgtca
    10681 cgttcagccg acgtattgat atgctgccat ttattgctgt cagatgaatt gctactccca
    10741 ctgttataac tccacgttga gttatcgcgc agtcgccaac taccaatgtt gatgccactc
    10801 tgtaaattca aatatgcata gttggatttt cctgcattat ggttgccacg attattaata
    10861 gaattaccat taaaactata gtttagtagc ccagcattta ttccttcttc ccacagatcc
    10921 ggagaaacat agccgcgagc cattctgcca acataaattt gcggaacgga gagtgataga
    10981 tgttgctgac caacattaaa ctcagatgat gcgtcagcca aacgatcccg aagtggtgtg
    11041 catgcgctgt tgtccgacac atgtttttct gccgaatgtt ccttattgtc taataatgct
    11101 gattttttta tgccaagact gacaagaagg tctgtactca ggcatgggat gagatctgca
    11161 ttattatcat ctgcaataaa agtaatatcc cgactagtca taaattcgtc gttcaaataa
    11221 atgtcgaccc gatatgtacc aggtggtgcc tcgcgtccct tagtaaaggc ggataaatcg
    11281 acagattcag caagatcatt gcttaaaaac cgtggattaa aataaaactc tgcgtcaacg
    11341 ggaaataccg cagttgctaa gccaagggca atggtaagcc gtgcaaagcg aggcagtagt
    11401 aacacctgat gcatacgata tgccttaaat gcggcacgaa aggctgtcat tattaattcc
    11461 gattgagttt attattaatt aggtcactgg cttactgcat ggttgcctta atacgcggag
    11521 ttaatgcacc gtaatcattg atcgtctgaa aactgatatc cccggttgca gcatgcggga
    11581 tatccaccga tacttcacct tttggcggca ccatggtgtt tgggagattg ctatttcctg
    11641 ctttcatatt tgttacggta atgaagtagg gcgttggatt aataagagtg agtttgctac
    11701 cgctgcgacg aaaccgaagc attgctggcg cttcttcagg tgccattgcc aggttcgtcg
    11761 ggcgataaaa catttttatg cggctaataa ttgccagctg taatgtattc tcattcttct
    11821 ggtctttttc catagcagga atagctttaa cattaaccca aaacaggctt tcacggtcgc
    11881 ctggtaactg atgattagtg gcgttgatta tccgaagcgt attctctttt ttaccttgca
    11941 tggaaaaaag cggtggggta atgacaaact gggtgtcttt ttggtcacct gcattttcta
    12001 tccatgactg aatgagatag acactggcag gatcattatt cgtgacaggc aataaaacct
    12061 gtttctggtt tgccggataa attacccgcg tcgcaccaag tgcgacagct gcgttggcaa
    12121 tggtgctatg gcaagcaaag agcaataaaa gtaacactgt cgccataaag gaaatagagt
    12181 aggggatacg cgtagtttgc atctgatgca ttctccatgt gttaagagga taagcgggaa
    12241 aaccgttaca tcagacaatc agtaaatgtt gctaatacat tggcgactcg ccgggtttcc
    12301 agacttaatg cgtttgtaat aaatgttggt tctcttttaa taggaaaacc ttgctacaaa
    12361 gtaaatattt taaataacag taagtttaac tactgttgtt tattgatcgt ttagatatta
    12421 tgtgattttt tcataccgct ccaaaacaca aactattcct cattaataat gagatgagca
    12481 ttggtgaatt gctcgggtgt gcgtagccat gccacacaca ccctgtttca atttcagatg
    12541 acgtatagcc accttaagat tgaattatag atattgaact ttgaacgtcg catcagcgtt
    12601 tgcagtacca gctactgact gcccgagagc aatataacga gcctggaatg gcagtatatt
    12661 cgtgccatca atcaagtcgg ttttagcgct aaaagtggca ccatcaagta ttaatacctc
    12721 ccccgtacgg tccaaaattt gaataccgac attttgggca gagcctgccg ctgaactctg
    12781 cagcgctaag gtatcgtcat tactggtgac agtagtaccc aggaatgcaa cagctgcatt
    12841 actggaaaca ttggtatcac aatcattcag cttgatattg aagccaacgg cggagctgag
    12901 gtcacctgct ttagccaggc gagaagaacg gacctgaccc agctcaaccg tttggttcat
    12961 tgattctgaa tcaatcgcac aagcggcatt taccacttca cctttaaaat gaataacacc
    13021 gccattaaca gtggtcgtta ctgattcatc accctcggct gcgcttaccg cagcagacga
    13081 catcgccaac acagaaacga caatcatacc gacatgtttg agtttcataa ttctccaaaa
    13141 tttatattta agtagctgaa taaagaaagt taacaatcaa gtcattctgt tattgcttat
    13201 aaaacaagaa tcattcttga tgggatattg ccctgagaat atagtttcca gggttattac
    13261 atttcacgaa gaacgataat atctttaaat tacaaggaca cctaatcaca acaagtttat
    13321 tgcagcactt gttgtagaga gtatttgcaa agacatttta tgtcgctaaa taacatgacg
    13381 atttcattat gcatgttatt tataaaccgt gactaaacag aatattattg cggacaaggg
    13441 aagtgtcagg aaaggtcaag agcgaccaat ggtttatatg gctataaata attagctata
    13501 gttaatgttt gcctggtgta actaacacgg aattgctcta aacagataag tgcacggttt
    13561 attaaagctg accgacagac tttcttaacc tggtaatatc tctcttaggt gactctccaa
    13621 acatccgcga atattcccga cagaaatggg ataagctttc gtaaccgaca gcataggctg
    13681 cagtggtgac atcgtaatgt tcattcagca tcagtcgcct ggcttcattg aggcgtagcc
    13741 atttctgata ccggagtgga ctcattcccg caagctgtct gaagtgttga tggaaagagg
    13801 gggtgttcat ttgcacgcgg tttgccagcc tgactcttca acgcagggaa gatgcggcaa
    13861 acggcaaacc tgcataccag ccaggcacag gccaatcatg aaagaggaga ggctttatgt
    13921 tctggagccc tgaagtaata acgacgaccg gaaaatcgct tcgcatcact caaattcaat
    13981 ttaacataat atacattatg cgcaccaaca taacccaagg gagaatccag tcgttggcgg
    14041 tcatgatgtc ctgctcatta acaacgacca aaaccccata tcttacttaa taccgtattc
    14101 tcggcttaac cggtcgtgca acctcaacac attggtttca actacatctt atccgctcta
    14161 cgggataaac gctgcgatat ccgcgatcgc ggataaaaag gaggatttaa aagtcgtttg
    14221 aagtcggcaa tattaagccg catgcatctt cgacatgcgg cttatacggt ttaccagcta
    14281 tatttcacac ccagcatgcc ctgagtatcg ctatagcctt tatcacctag ttgcacaccg
    14341 acattccccc acaggctaag gttgttatat tcgcatcgtt ctgataccag gtcgcgtaca
    14401 gtcctgcgct gtaaccgctg atgcgcccat ccgatttata acccacatga ttactctgag
    14461 tattactgtg ctgattggca tagcctgcca tgacgccgag atgccagcga tcctgcgtgc
    14521 tactccactg cgccaaatcg ccgcctagct gcaatacata gcggttagcc tgagtattta
    14581 gctgaccatc accggtcctg aaacgttcgt gccctccgac atgacgcatc cacatactgg
    14641 ttgccgaatc ctgaactcgc agtgaatctg tatactgcgg ctcacccaga cggtcgtgta
    14701 agcgatggct aaacagcgag ttggctgcgg caatgttgct gatatagctt ccggcctccg
    14761 ggcgataaac tgatgggcct tccggatcca caacaggggg attattgatg ggatcgggtg
    14821 tatccgctgg cgttacgccg tcccatttac tggtcagata ccagtttttc tcgtcattcc
    14881 ccttcccttt agccagcgtg tagacgtaag ccccagcttc gacagttccg gtggtcagcg
    14941 cgaagttacc tgcagaatta ccgccaacct caatgagttc aataccgttg accgtttgtg
    15001 caccgacgcc gccaatgtta tcaacccgaa cgcgagtgtt cccttgggta tcgcctttca
    15061 cgttcatttt atccgtgggt gaattatcgc cgcccagcgt cgcgttgaac gtcatcaggc
    15121 cattgtttcc ggtatagttg ccgttaacgg tcagtacatt ccccggtttc cccccttcgc
    15181 tgttcatttg aaccgtgcca ccattctcca ggttaccgcc gacagtggtt ttagcgacac
    15241 gcagtgtccc ccctggcata acgttcacat cacctgcagt ggtgacattt cccgacatca
    15301 caccgctgtt atggatatta atgtgctgac ttgccatatt aatggcagag tcggaaccga
    15361 aagcaatttc accacccacg atatccgtcg taccctgata cgccgcggaa tctttgttaa
    15421 gagtcaggat cccggtaccc agttttgtca catcaccgct accgctgata ctgtgcgtca
    15481 tcgcccagct acgctcagtg gcgatggtga gcaggccatt attgataacg gaagcatccc
    15541 ccaggttttt ctgctcgttg acgctgagcg cgctgcctgt atcaatgttg aattgcccca
    15601 caaagcggct attatcgcca tccagctcta cgtctgtcct ggcggttgcg ctcacgatac
    15661 ccttcccgct tatgctatta cgcagttcgc cggtcacgtt ttttagcgtt aacagaccgt
    15721 cattggcaat atttcctctg cctaacccct gcgtattatc gaggctgact tccgcatttg
    15781 ggctaacgct ggttaacgca ttgtagcgtg cattaagccc ctcgatagcc agcgttcccc
    15841 ctgtaacatt caggtttccg ccacctgtca gttcaccctg actgatccca cctttattta
    15901 ccgtcagcga cccttctttg aacaaaacag tcgaacccat ctgcccggtg aatgtctcta
    15961 ccgtctgcgt cgatccatta agatcgacga tggccgcgtt gctgatgttc agttcccggg
    16021 tgttgcccag cgcgccatcc gcatcggtac gtaatgtccc catctgaacg taggttgccc
    16081 cctgatagtc gttctgaccg ttggaaagcg aaacctgtcg caccgtattg attgccagat
    16141 ccccttcacc accgattttt gccgacatat cggccgttgc gccataggct ccgccatgtt
    16201 cggctaatgt cagcttttgc ccaccatgga tgttcagcgc tttcagccca tagttaacgt
    16261 acaaaccatc acctggtgcg gtcgtgaggc ggaatccata atcaccctca gcgaccaccg
    16321 ttccgttttg cgcaatgtgt aacgttttgt ccgcttccac ctcgtcgccc tgtaaatcac
    16381 gtaacgttaa tgagccaccc gacccaataa ccgtttgcgc cttcaccagt tgaacgccga
    16441 catggttatc atcgtgttcc agcaaattga gcgtcgtcag agggttattc gccatgggat
    16501 cattccacgg tttaggcacg tcgataagca cgtcgcccgt cccgtttacc tgatagttac
    16561 ggcctttcca ggtgtagtca ctcgcgccga caaccagcgt atcgacgctg atatatccct
    16621 ctgcaagcgt cgcagcagga atatccgtat cgaaaatgag cgtaccgcca ttcatggcca
    16681 gtccaccaat ggattgctct cctacgttta ccgatgtggt attttcaatg tcagactgca
    16741 acatcgcgtg agtaagcgca gcggtgttgt cgcgttccag agtgaaggta ctgtctttca
    16801 gttgggcaac accggcgaat tcagtccctg ttgcatgggt aaagccaaac atcttgtcgg
    16861 aggatgacag ctgcactcgc atcaagccat cgcctttgag cgcattggtc agcgtgtaat
    16921 ctcctgcgct gttcgttgat gcgagaatgt cgagcgtacc ggggcttgca atggtgactt
    16981 cactcgaacc catgcctgtt accccatctg ccgtatgtga cgcaatagtc aggaggccgt
    17041 cattgaccag cgttttaccg ctataagtat tccctgcatt gaacaccgta tggcctgcat
    17101 catgcatcac ttttccgtct ttatcgtcac ccgtaatcag catatcgacc tggtagccgg
    17161 catcactgtt attggtatga ttaaagacaa aaacgccttc gccaagacca aactccactt
    17221 tcgtcgcatt ggtaataaac ccggcatctg ccgccacctc accgtgagca gcaccaatgt
    17281 ttagcgttcc tacagcaggc tcaaaaacac ctaagtaaac ttctccacct tcgacattca
    17341 gcgtgccgtt attcgtcagg gttaacgtac ctgtgccagt cgtccctacg ttcatattga
    17401 atgtttcaag aagagagttc tgcccatcca cccgcacgtc gcccttactt ttgtcattca
    17461 gagctatctg tgtatcacgc gctttaacta tacctccggt ggtaatattc agttcacccg
    17521 tacctaatac accgacctgt agcaattggg cgttagtaga tgacgttttt aaattccaga
    17581 gactatccgt tgaaatatta actatcccct taccggaggc atttcctccg ataaatccat
    17641 agcccgttga attaagggtg gcaccattac tgacatttac gatgccgcta ccaaaattac
    17701 cgacattgat acctacagta tcgaacgatg atgtcgcatc catcaggttc agtgtgccga
    17761 taccgcttgc tttatcgccc aaaaaagtaa tattttgcga tacgaactta ccgccatcgc
    17821 tgatattgag gtttccgacg cccgcaacac caatagtggt atagacattc ttattgacct
    17881 cccacatccc ccccgtggtg atgctaacct tcccgatgcc ggtttctcca taaccgagtg
    17941 aacttcctcc gttgctgaca acaagcccct cattactgat attcatttcg ccgtggccgt
    18001 cataaccaag attagttccg agattagtga taacggagtt cttatcctta acagtaatgt
    18061 tgcctgtgcc tgtttctttc atccccgcag tgataattcc cgaatctact ttgccttcac
    18121 tagagacatt aagttcaccg tcgccagcat caccgatata gatatagcgg aaagcttcgc
    18181 ccgttccgag gaaattccaa tgccctttat cggtcacgtt gacgacaccg tgggaaccgt
    18241 cctgaacgcc caccaatgaa tattctttgt tattaatcag tccattattg gaaatattga
    18301 ctttgccatt accgaaatat ccattataga gtctgcgtac cgtgatgaca gagtcttgat
    18361 cctgcacatt cagggttccg ataccggtgg cattgcctcc gataatcgta ttttcagccg
    18421 taatcagtcc accctcgcga atagtcgctt ccccagttcc ttgattacca atttgaaatt
    18481 caattgagga atcgttattt tttattagcc actcaccgcc cttttcaacg acaaccttac
    18541 cattactgtt cgcttgatat cctacagtgg cgactattga actcgtgacg taacccttat
    18601 ccgtaatatt taatgagccc gtaccgtaac tccctatttc gaataattcg gtcgtcagaa
    18661 ccgagtcctc tccctcaaca ttaaccgtcc cgacgcctcc tgtcgaagaa cctatcctta
    18721 aatatcctcc gtcaacgtga cccttctgtt taatattcag cgtccccgtt ccggattgac
    18781 ccacatttaa aggccttgca ttatttccgc tatcatacaa tcgccaggtg ccacccaaaa
    18841 cattaacggt gccctccgag tcctcattac caccaatgac gctggtagta atagtggtta
    18901 aggaggcatt accgccagcc aaaatattaa gcactccagt gccataccac ccaaccaggt
    18961 aggcatcgta agcaacatca gtattaatat tggtaatctg accattatca acttccagac
    19021 tcgcaccaga cgctgaaccg cttagtgcca gcaaaacacc cagcgtcaat ctactgaatt
    19081 tcgttgtcaa tccagaggat ttacgcaaat taaccgtcga tgttttacct accctgcgag
    19141 ttaattccga gcaggcctga aatacctgta gggtgcaatt ccatatcacg cgatagattc
    19201 tattcatata aacgctccat atacaaacaa tacagccacg aaagagatga gtcgtcagac
    19261 attaaaaatg aaacttatta aattgtcaga ggtctgtatt gagtgttggt tggaggtggg
    19321 aaggtgtcaa tttggctaac tttctaatgc tttcgaattg atagattaaa aatcgcatag
    19381 ttcttatttt atttaaaaat gaaccatgag ttattgttgc ttaataagaa aaatatttct
    19441 ttcaataaga aaattaccat catcatattt aacaaagaat agcactaatt gctaaaaaag
    19501 aactctatta aaactctttt tcattcattt atgcctttag gaatattcgc tataaaataa
    19561 gggttatgct taatgtttta agaaaaatag caatttcccc ttgaatatag tactggcgat
    19621 ggaacgatga atctgcaagc tggcatttaa caagccagtt ctaaaagaag ggaaataaga
    19681 ataactatac tcaaaaacta acagccacgg tcatcatgat gtggctgtca atgaaactat
    19741 aacccaggcg ctttccataa cgacgtcgtc agtccatgat caaccagccc cagctgatcc
    19801 tgataaactg cctgccactt atcgcgtgaa tcctgataaa gttcatgctt ttctgggtct
    19861 ggtgtgtgcg tccgttccca gcgaaccagg cgttctccgg tttctgccat tgatgaaaaa
    19921 attccggcac cgacgccagc tgcaatggca catcctaatg cagtggcttc tttgaccacc
    19981 ggaatattga cgggtaatcc cgagacatca gcgagaattt gactccataa tttccctttt
    20041 gaacctccgc ctgcaaagac cagagatgtg ggatgaatat tcgaaaaatc agcaatttgc
    20101 tgcaagttac acgctgatac aatcgacgca ttttcttcca gcgcacggaa caatgtcgct
    20161 ttgttacatt tatccgggtc aatggacaag ttaataaagg aaggcgcagc gtgataccag
    20221 gttttaaagc gcattctgtc ggagaatatc ggcattacgc cccacgaccc aggcggcaca
    20281 cgacttgcca tctcttcaag cagcgtatag gtgtcgatgc ctaaacgttc ggcaatcagt
    20341 ttttcttcgg cacagaaagc atcgcggaac cagcgcatgg tgagtccggt aaaaaagctt
    20401 atagattcag cttgtaccat gccaggaata acatggggat taacgcgcac gttcatttct
    20461 gggtctgtca ccggcgcggc taagtttaca acttgctgcc agaatgtgcc gccaagaacc
    20521 gcggtttgtg ccggacgtac aacgcctaac cccaggcaac caagctgcac gtcacctcct
    20581 ccaacgacca ccggagtgcc cgccttcaga ccgcagagct ccgccgcgtg tgaacttacc
    20641 acgcccagca atgtgccggt ttctttgaca ggagaaagaa tatcggcacg caggccagcc
    20701 atatccagca atgcaggttt ccagtcacgg gtggttagat caagaagtcc cgtggtgcca
    20761 gcgttagagg gatccaccgc cagttcgccg ctgagcatat aggccagcca gtcgctgatc
    20821 atggtgatgg tcgacgcctg tcggtagata tcggaacgat ggtgcgccag ccaaagtaat
    20881 ctggggatgg cgcttaaagc cagcgtttgc ccggtcgctc gataaacttt gttttcaaag
    20941 gtattgttgt gcagttcttt aagttcgcta acttcgcgcg ccgctctggc atccacattg
    21001 gcgcaggccc agatcggtgt tccatcattg ttatataaaa caatgccttc acgcatcgaa
    21061 catgccgaaa cggcagcgat atactccggg gctatgccgg cgttgtgcag cgcctggcgc
    21121 atacactcac acgccagttg ccagtttttg ttgagatcaa attccataga accaggaacg
    21181 tccggtactg ccagatgccg ccactccgcc tgtcccacgg ctatttgatt gccttccagg
    21241 tcgaatatca cagcccgaat acttccggtg cctgcatcca gcgccatcag gtagtacttt
    21301 gattctgaag gggtaaagag tcgagccatt agattatcct cggcttatgc gttctgtctg
    21361 ttcctctata cgttctccat cattcccggt aataaggtca tgcaaattta actacgtaaa
    21421 atcgccgctg ctgtatcctg atcggtaacc agtgcgttga tataaccgcc tttcattgca
    21481 gcggcaattg cttcggcttt attttctccc cctgccacgc caacccggac gggtatggtc
    21541 ttcagcgcgc ttaaaggtaa gccaatcagt tcgttatgta ttttaatatc cgtgacaacg
    21601 tcaccttttg catcaaaaaa gtagcctaaa atgtcgccaa ccgccccttt tcgaccaatc
    21661 attaactgtt cgccctggct gatataaccg gagcgaatga tcgtcgcgtc gtcttgttga
    21721 ctcacggccc caatgccgac aatcgccaca tccgcagctt gtgcggctag cagaacatct
    21781 ttgacgcaat tttcattttt tagcgtatgg gcaatgtcag cggaggatgc ccgcaacgga
    21841 gccggaataa tattcacact gcacgccgcg ttaagctgcc cgatacccgt catataagaa
    21901 ccgactccac cggagagcgt gaccaggcga atttgctgtg acgaaataaa accacttaag
    21961 cgttgcagcg tattcatggt tgcctcgcca aaaccaatcg ccagcatctg ttgtggttga
    22021 agtaaactca tcaacatatg cgccgcgcct atccctagtc gcccgccgac atcagcatcc
    22081 gcaagccctg ggatcacccg gacatgttgc agcgaaaact gacgacataa ttgtgtttca
    22141 tattccagac agccttcaaa gcgtgaatta atctgtacgc gaataatgcc ggattgatgc
    22201 cctttctcca gtaatcgcga cactttcaaa cgggtcaggc cgagacgatc gctgatctca
    22261 ctctgggtca gcccgtcgtg gtagtaaaac cacgcaatcc gtgcgacctg ttcttcttca
    22321 cacattccct gttctgaaat taccgaatcg ttgattgtca taattcattc ttcactttga
    22381 acatacttaa atctttaatg caattgttca gttcttgcgc atttatatct gtgatggcaa
    22441 ccacagtttg actctatgag catgaacaaa cgcaaccgtg aaaatcaaaa tagcataaat
    22501 tgtgatctat tcgtcggaaa tatgtgcaat gtccacctaa ggttatgaac aaattaaaag
    22561 cagaaataca tttgttcaaa actcacctgc aaaactgaac gggggaaata tgcaaacgag
    22621 tgatacccgc gcgttaccgc tactttgcgc ccgctcggtt tataaacagt attcaggagt
    22681 taatgtcctg aaaggcatcg attttacgtt gcatcagggg gaggtccacg ccctgctcgg
    22741 cggcaatggt gccggtaaat cgacgttaat gaagattatt gccggtatta cccctgctga
    22801 tagcggtacg ctggagattg ggggcaacaa ctacgtcaga ttaacgccag ttcatgctca
    22861 tcagctgggc atttatctcg ttccccagga accgctgctt tttccaagcc tgtcgataaa
    22921 agaaaacatc ctgtttggtc tggcaaaaaa acagctctcc atacagaaaa tgaataactt
    22981 gctggcggcg ctgggctgcc agtttgatct gcatagtctg gcaggatcgc tggatgtcgc
    23041 cgatcgccaa atggtggaaa tcctccgcgg gctgatgcgc gattcgcgaa ttctgatcct
    23101 cgatgaacct accgcctcgc ttactcccgc cgaaaccgaa cgtttgttta ttcgcttgcg
    23161 ggagctgctt gctactggcg tgggtattgt ttttatctcg cataagctgc cggaaattcg
    23221 ccagattgcc gaccgaatta gcgtgatgcg cgacggaacc atcgccttaa gcggcaaaac
    23281 cagcgaactg tctaccgacg acattattca ggccattacg ccagcgctac gggaaaaatc
    23341 gctctctgcc agccaaaaat tatggctgga attacctggc aaccgcccac aacatgccgc
    23401 gggaacgccg gtgctgacac tggaaaatct gaccggcgaa ggtttcagga atgtcagcct
    23461 gacgctcaat gccggagaaa ttctgggcct ggctgggctg gtgggagccg gacgcacaga
    23521 actggccgag acgctctatg gtctgcgtac tttgcgtggc ggacgcatta tgctgaatgg
    23581 taaagagatc aatagattat ccaccggaga acgtttactg cgcggtctgg tttatctgcc
    23641 ggaagatcgc cagtcatccg gacttaatct cgatgcttca ctggcgtgga acgtctgcgc
    23701 ccttactcat aaccttcgtg gattctgggc gaaaaccgcg aaagataatg ccaccctgga
    23761 acgatatcgt cgggcgctga atattaaatt taaccaaccg gaacaagctg cgcggacctt
    23821 atccggcggc aaccagcaaa aaatcctgat tgccaaatgc ctggaagcct cgccgcaagt
    23881 attgattgtc gatgagccga cgcgcggcgt ggatgtctcg gcgcgtaatg atatctacca
    23941 actgttgcgc agcatcgccg cacagaatgt ggctgtgctg cttatctcct ctgatctcga
    24001 agagatcgaa ctgatggcag atcgcgtgta tgtgatgcat cagggcgaaa ttgcccactc
    24061 tgcactgacc gggcgcgata ttaatgtcga gaccattatg cgcgttgcct tcggcgatag
    24121 tcagcgtcag gaggcgtcat gctgaaattt attcagaaca accgtgaaat cacggcactg
    24181 ctggcggtgg tgctgctgtt tgtattaccc ggttttctcg accgtcagta tttaagtgtg
    24241 caaacgctga ccatggttta tagcagcgcg caaatcctga tcctgctggc aatgggcgcg
    24301 acgctggtaa tgcttacgcg caatattgat gtttcagtgg gttcgattac cggaatgtgc
    24361 gcggtgctgt tggggatgtt actgaacgcg gggtattcac tacctgttgc ttgtgtcgcg
    24421 actttactgc ttggtttgct cgcgggattt ttcaacggtg tcctggtcgc gtggctaaag
    24481 atccctgcca ttgttgccac gctcggtaca ttagggttgt acagaggcat catgttgctg
    24541 tggactggcg gtaaatggat tgagggatta cccgccgaac tgaaacaact ctccgccccg
    24601 ctgctgtttg gcgtttccgc gattggttgg ttgacgataa ttctggtggc atttatggcc
    24661 tggctgctgg caaagacggc gtttggacgc agtttttatg tcacgggcga taatttacag
    24721 ggcgctcgtc aactgggcgt tcgtacggaa gccattcgca ttgtggcatt ttcgttgaac
    24781 ggctgcatgg cggcactggc gggaattgtg tttgcttcgc agattggttt tatccccaac
    24841 cagaccggta ccgggctgga gatgaaagca attgcagcct gcgttctggg cggcattagt
    24901 ttgctcggcg gttccggcgc gatcatcggt gcggtactcg gcgcatggtt cctgacgcag
    24961 atcgatagtg tactggtgct gttgcgcatt ccggcatggt ggaatgattt tatcgcgggc
    25021 atggttctgc tggcggtgct ggtgtttgat ggacgcctgc gttgtgcgct ggaacgtaat
    25081 ctgcggcggc aaaaatatgc ccgctttatg atgccaccgc cacccgttaa acccgcttcg
    25141 tcaggtaaaa aacgggaggc cgcgtaatgc gtattcgcta cggttgggaa ctggctcttg
    25201 ccgcactgct cgttattgag attgttgcat ttggtgcaat taacccgcgg atgttagatc
    25261 tcaatatgtt gctgttcagc accagtgact ttatctgcat tggcattgtc gccctaccgc
    25321 tgacgatggt gattgtcagt ggtgggatcg atatttcgtt tggttcgacc atcggcctct
    25381 gcgccattgc attgggcgta ctgtttcaaa gtggtgtgcc gatgccgctg gcgattcttc
    25441 tgaccttact gctcggcgca ttgtgcgggt tgatcaacgc cggattaatt atctatacca
    25501 aagttaaccc gctggtgatt acgcttggca cgctgtatct gtttgccgga agcgccctgc
    25561 tgctttccgg gatggccgga gcgacggggt acgaaggtat tggtggattc ccgatggcgt
    25621 ttacagattt cgctaacctg gatgtgctgg gactccccgt tccgctgatt atcttcctga
    25681 tatgtctcct cgttttctgg ctctggctgc ataaaaccca tgccggacgt aacgtgtttt
    25741 taattgggca aagcccgcgt gtggcggttt atagcgcgat tccggtaaac cgcaccttgt
    25801 gtgcgctcta tgccatgacg gggctggcgt ctgcggtcgc cgctgtgctg ctggtatcgt
    25861 attttggttc agcacgttcc gatctcggtg cgtcgtttct gatgcccgcc atcaccgccg
    25921 tagtgctcgg cggtgccaat atttatggtg gttctggttc cattatcggc accgccattg
    25981 cggttttatt agtgggatat ttgcaacaag gtttgcaaat ggcaggagtg ccaaatcagg
    26041 tgtccagcgc cctttccggt gcgctactta tcgtcgttgt cgtaggtcgt tccgttagcc
    26101 tgcatcgcca gcaaattaaa gagtggctgg cgcgtcgggc caataaccca ttgccataaa
    26161 ggatatcttc atgacacttc atcgctttaa gaaaatcgcc ttacttagcg ctcttggcat
    26221 tgccgcaatc tctatgaatg tgcaggccgc agagcgtatt gcatttattc ccaaactggt
    26281 tggcgtggga ttttttacca gcggtggcaa cggcgcacaa caagcgggta aagagctggg
    26341 cgttgatgtg acctacgacg ggccgacaga acccagtgtt tctggtcagg tacagttgat
    26401 taataacttc gtcaatcagg gttataacgc cattatcgtt tctgcggttt cgcctgatgg
    26461 cttgtgtccg gcactgaaac gcgccatgca acgtggtgtg agagtgctga cctgggactc
    26521 tgataccaaa ccggagtgcc gctcttacta cattaatcag ggaacgcccg cccagttggg
    26581 aggtatgttg gtggatatgg cggcgcgtca ggtgaataaa gacaaagcca aagtcgcgtt
    26641 tttctactca agccccaccg ttacggacca aaaccagtgg gtgaaagaag cgaaagcgaa
    26701 aatcgccaaa gagcatccag gctgggaaat tgtcactacg cagtttggct ataacgatgc
    26761 cactaaatcg ttacaaaccg cagaaggaat attaaaagcc tatagcgatc tcgacgccat
    26821 tatcgccccc gatgccaatg ccctgcccgc tgccgcacaa gccgcagaaa acctgaaaaa
    26881 tgacaaagtt gcgattgtcg gattcagtac gccaaatgtg atgcgtccat atgtggaacg
    26941 cggcacggtg aaagaatttg gcctgtggga tgtggttcag caaggcaaaa tatcagttta
    27001 tgtcgcggat gcattattga aaaaaggatc aatgaaaacg ggcgacaagc tggatattca
    27061 gggcgtaggt caggttgaag tctcgccaaa cagcgttcag ggctatgact acgaagcgga
    27121 tggtaatggc atcgtactgt taccggagcg cgtgatattc aacaaagaga atatcggcaa
    27181 atacgatttc tgatgtgcat tacttaaccg gagtacgtta tggcagattt agacgatatt
    27241 aaagatggta aggattttcg taccgatcaa ccgcaacaaa atatcccttt taccctgaaa
    27301 ggttgcggtg cactggattg gggaatgcag tcacgcttat cgcggatatt taattcgaaa
    27361 acgggtaaaa ccgtgatgct ggcttttgat catggttatt ttcaggggcc gactaccgga
    27421 cttgaacgca ttgatataag tatcgccccg ctgtttgaac atgccgatgt attaatgtgt
    27481 acgcgcggca ttttgcgcag cgtagtcccc cctgcgacca ataagccggt ggtactgcgg
    27541 gcgtctggtg cgaactctat tctggcggaa ttaagtaatg aagccgtggc gttatcgatg
    27601 gatgacgccg tgcgcctgaa cagttgcgcg gtggcggcgc aggtttatat cggcagcgaa
    27661 tatgaacatc agtcgatcaa aaatattatt cagctggtcg atgccggaat gaaagtggga
    27721 atgccgacca tggccgtgac aggcgtgggc aaagatatgg tgcgcgatca gcgttatttc
    27781 tcgctcgcga ctcgaatcgc cgctgaaatg ggggcgcaaa ttatcaaaac ctattatgtc
    27841 gataaaggtt ttgaacggat tgttgccgga tgtccggtac ccattgttat tgctggcggt
    27901 aaaaaattac cggagcgcga ggcgctggaa atgtgctggc aggctatcga tcagggcgct
    27961 tctggtgtgg atatggggcg taatattttc cagtctgacc atccggtggc gatgatgaaa
    28021 gccgtacagg cggtggttca ccataacgaa acggctgatc gggcatatga actctatctg
    28081 agtgaaaaac agtaactgcg gatttaagga gaagaattat gcacgtcaca ctggttgaaa
    28141 ttaacgttca tgaagacaag gttgacgagt ttatcgaagt ttttcgccag aaccacctgg
    28201 gctcggtaca ggaagaaggc aatttgcgct tcgatgtctt acaggacccg gaagtgaatt
    28261 cgcgctttta tatctacgaa gcttataaag atgaagacgc agtggcgttc cataaaacca
    28321 cgccccacta caaaacctgt gtcgcgaaac tggaatcttt aatgaccggg ccgcgtaaaa
    28381 aacgtctgtt caatgggttg atgccgtgag gcgaatttat caattttatc tacaattggg
    28441 gtaacgcgct gacgggagta aaaaaatgtc tgactggaac ccctctttat atctgcactt
    28501 tgccgctgaa cgatcgcgtc cggcggtgga gctgcttgcc agagtgccgc tggagaatat
    28561 caaatacgtt gccgatcttg gctgtggccc aggtaacagc accgccctgc tgcatcaacg
    28621 ttggcctgcg gccaggataa caggcgtcga ctcatctccg gcgatgattg ctgaagcgcg
    28681 cagtgctttg ccagactgcc agttcgtgga agcagacatc cgcaactggc aaccggaaca
    28741 ggcactcgat ctgatttttg ctaatgcctc actgcaatgg ctgcccgacc actacgaatt
    28801 gttccctcat ctggtttctt tacttaattc gcatggtgtg ttggcagtac agatgccaga
    28861 taactggcta gagccaaccc atgtgctcat gcgcgaagtc gcctgggaac aaaattatcc
    28921 ggatcgcggg cgtgagccgc tggctggcgt tcatgcttac tacgatattt tgagcgaagc
    28981 cggatgtgag gtcgatatct ggcgaaccac ttactatcac cagatgccgt cgcaccaggc
    29041 gattatcgat tgggtgaccg ccactggatt acgtccgtgg ttacaggatc tgaccgagag
    29101 cgaacagcag cattttctta cgcgctacca tcagatgctg gaagagcagt atccactgca
    29161 agagaacgga gagatactgc tggcatttcc gcgtctgttt attgttgccc gccgtacgga
    29221 gtaattatca cgtcagctgg taatggcgat cgggaagaat tttcgctgga atttctgctt
    29281 catcgttcat ctgtaacagg tcaatttcaa tagcgttgca gatggcatcc agcggtaaat
    29341 cattgttttc agtaccgaag ggatcttcca gttcttccgc cagacaatcc agcgaaataa
    29401 aagtgtagga aatcagcaca gagataaaag gcgtcatgta atgcaggtcc acgaccagcg
    29461 caaacggcag catgatacaa aacagataca cggtgcgatg caaaatcagg gtgtaggcaa
    29521 agggaattgg cgtataggca atgcgctcgc atcctgccag gaccgctgaa atatcattaa
    29581 gccgatcgtt gaggctaata aacaggatat ctgaaagttg tccattgcgt cgctgaaccg
    29641 ccaaccactc ccccattatt aacaagatgc ggttagctgg agagttcgaa gccagtacac
    29701 gctgaagatc ttcagtcttg agataatgag ccagcacttc cgcctgtggc tgtttgcgta
    29761 atgtcatgcg taaacagtgg gcgaaggcga tttgcagccg ggcaaactcc cttacacttg
    29821 ccgaatccgg taatgtcgtt tttacctcgc gcagtaacga ccgcgaggca atcatcaact
    29881 gcccccaaag ttttcgcgct tcaacgtaac gcgcgtaccc ggcattatta cgaaaaccaa
    29941 gaaaaatggc gatggcgaca ccgagaatgc tgaacggtgc gagggtgaat ttaatgccca
    30001 aatgcgtgta ccagggcagc atgaaaataa cagcgatcga aaagagaaaa ttgagtagta
    30061 agcgcgagga tatcttggat aatactgaac cgtgccagac aaaaatacgg cgcagccagt
    30121 gttgttgtgg acgaacaatc atggttatct tcaggcgtgg aaaagtcgcc ctattaaacg
    30181 tgattacgat cacattctca agacactact tacaaattac ctacaagctt acaacattta
    30241 caggtgtggc atatggcacc cgttaatgat aaaggcacat aggatgttgt aactaatata
    30301 gtgaaataag aaacccggtc gaaaccgggt tcagagtatt agcacaacgg acgtacagct
    30361 tcgcgcattc ctttttcgag aatcgcatcc aggtcattag ccacctgctc gaccagacca
    30421 ggcacttgcg tcaggtcctg ctcccagtgg tctttttcag ccaacacaat agcaaccagt
    30481 tcctgggtgc cgataacacg gtcacgatgc tggctccaga gttgctgata acgttccagc
    30541 cagtgggcat cgtcctgtac cggatatgtt tctccattac gctcaccgcg atagaacgca
    30601 atcaatgctg ctaatgcgaa agtaaggcgc gccggaagtg tgccgtttgc cttctgccct
    30661 gccagcagct gcggcaggat acgggtgcgg aacttggtca taccgttaag cgcgatagac
    30721 agcagctgat gcttaatgta cggattacgg aaacgtccgg tgactgcact ggcgaaagat
    30781 tccagttcat cacgaggcaa atccagtacc ggaataatct cttcgtagat ggctttttca
    30841 acgaatgcac aaatttcagc atcgttcatc gcttcaccta cggtatccag ccctgcctgg
    30901 aacgccaccg gcaccagcgc ggtgtgcgca ccgttgagta ttgccacttt gcgttcttta
    30961 tacggtttaa tatcgtcaac aatcagcacg ttgagcggat atttgtccag acgcagttca
    31021 gtcgctaagg atttcggccc ctgaatcaca aacaggtaaa agtgttcagc ggtgtcgagg
    31081 aaaccatcgt gataacccag ttcttcttcc agtttagcca cttcatcacg cggatagccg
    31141 gtaacgatac ggtccaccag cgtagaacag aagctgttag cctgatccag ccattgaata
    31201 aatgcttctg gcagtgccca ctcttgcgca tagcgcagca ccagttcacg caacgcgtcg
    31261 ccattgtagt cgatcaactc acacgggatg atgatccaac ctttatccag cgcaccgttg
    31321 aaatggctaa aacgttcgaa cagcagacgg gtcagttttg ccggataact taccgctggc
    31381 gcgtcatcga atttatcgcc cgcatggtag ctgatacccg cttcggtggt gttagagaaa
    31441 acaaagcgca tttccgggtt gtgcgccagt ttcaggaatt catcgtattc actgtagacg
    31501 ctgatttcac gattaacaga gcgaatcaga cgcgcgtcgc tgaccgcttc ccccttctcg
    31561 ttcagaccac ggataatggt ggtgtacaga ccgtcctgcg tgctcagtga cggcgggaat
    31621 gaagtttcaa tcggacgaac aacgaccacg ccagaattca gatcggtgtg ctcattcagg
    31681 agatcgattt gccagtcaac aaaggcgcgc aggaagttac cttcaccaaa ctgaatgata
    31741 cgttctggat actgtgcacc gggaaaatcg cgacgattta gtgttttcac aatgggttcc
    31801 cttctgatta gtcatacaac ctgtttgaat tggtacgaca ggttagcaaa ctttaatacg
    31861 ccgaacccct gttttgatca actcctgatg attaatgagc agttttatga gaaaagtgtg
    31921 gcgcggatca tggtttaatc gaggaactac gccttttatt cgctcaataa agtggtagaa
    31981 cacattccat ttaaatcgct gtgaatgaat aaagatgtca gacaacttcc tcaccgtaac
    32041 gcatagtgct ggtacggttg cgcccatctt tcttcgagcg atacagacat gtatcggctt
    32101 cgaccatcaa tttattaaag tcatcggtca gcgttcggta cgatgcgcaa ccactaccga
    32161 cgccaatact taccgtgaga taaagcgttt tttgttgcca ggtgaacggt tgcagttcaa
    32221 cgcctttacg gattttttcc gccattagca gaccatctac aggattcacc gatggcactg
    32281 caacagcaaa ttcctcgccg cccattcgcg ccaccagccc cttatcaccg acaatcttct
    32341 gaatatgctg ggcaaacacg cttaacactt tatcgccaca ttcatgtcca tagttatcgt
    32401 taatgctttt gaaatagtcg atatcaagca gcatgacagt cagatgttgt gtctgtttca
    32461 gcgatggact tttcagcgcc tcataaagac cggaccgtga gtaaacctga gtcagaaaat
    32521 caaagtcggc tcgcagcgca acttgcttca ttagcgaatt gatcgctgcc acgctaaaag
    32581 aaaccataat tgggcatatc gccatcgtgg caataccgag acgtgcggag aacatttctg
    32641 gaatggagaa tggcgaaccg accgagatat caatcaccga atttgccacc agtacgattt
    32701 ccaccgcacc ggtgacaaag gtcaacagac atgttacctg cggcgtatag cgcactgcac
    32761 accagattaa tgcagggagc ggaaacgcca gactccccgc tccgccaatg actaccgagg
    32821 caataaccga cacaataagc gcgatagcag gcatcatctg ctctgcttta aagcgcggca
    32881 gtactccagg aattgccaac gtcagcatac aaggcacgat caacacgccc gttgagaatt
    32941 gctcactgaa ccagtcggca agcaaaggcc agaaatccag actatcaata ctgaccgaac
    33001 caatcgcccc gacaatagcg cataataatg cggcaatcag acagtaatta aatagccgta
    33061 aggcacttac tggctcatac ttatttttcc ccagacgctt atcccgcgcg accagtaagg
    33121 cgacggtaac aataaacatc atattggaga aattgatagc cagtgaaacc agcccccatt
    33181 cggtggttat ggcatcataa accagcatcg ccacataact gatcgcatag taatgcagtc
    33241 gattaagcca gacatagcgg gcaaacaccc ccgccattac gccattcagc ggccagaaaa
    33301 gagaaagttc gtgaaccagc cgtaactctg caccaataaa atagaacagc gtggttagca
    33361 caaaaatagc gatgctatta cgtagcagat gaccttcctg gaacaaacgg aaagttgaaa
    33421 tgggctgtac atgcattaat tatctgtcca tacaattgcg ggtggcaatt atctcaacgc
    33481 aataagtaaa ttcttagaaa ggatagcatg ttcgctgaca acggcggcaa ctaaagatac
    33541 ctaatgaatg ccattttatc gatttccgta attatcatgg ctgattcatc tgtataacca
    33601 accagtgata gattaccgcc acgatataac gttgttgttc ttcgcttaac gaaacaccct
    33661 gcggaatgtt atgccactta gccagacaac cgcgacaaca ggttgcagtg gcgtgctgag
    33721 cgataaacac cggatgcccg cgcatggggg tttgcttacc gtcattagcc ggtaatgccg
    33781 gagcaagacg tttagcgaca aaatctgccg catgttgttc aatgactggc gcgcctttct
    33841 ccagacaata ctgacgctct ttcacaccca aacgaaagcg agagcgaaat tttgaacggg
    33901 ataaacgcgc gaagagtgga tcgagagact gcattaataa actgaacgcc ctaattgttt
    33961 cgtcaattgt tcaaggacag caatgcccgc aagcgagtta ccggcatcat ccagttccgg
    34021 gctccagact gcgatggcca tttcatgcgg aacaatcgcc acaataccgc caccaacgcc
    34081 agattttgcc ggtagtccca cccgccaggc aaactccccc gcgttctggt acataccact
    34141 ggtcgccatc agcgcgttaa tttgccgcgc ctgcattggc gtcaccacag gttcatcaat
    34201 atgaatagct ttcccctgat tagccagaaa gacaaatgtc cgggccagct ctacgcagct
    34261 cattttcaag gcgcaatagt ggaaatagtt ttgcagaacg gttgtcacat catgatggaa
    34321 attgccaaac gacttcatca gccaggcgat agccgcattt cgcgcggaat gttcaaattc
    34381 ggaacgcgct accaccgtat cgtaggaaat atcagacaca ccacttaagc cgcgcacgac
    34441 ttccagcata cgttgccgag gtgcgcttaa tcgcccttgc aacatatcgc agaccaccag
    34501 cgcaccggca ttaataaacg gattacgcgg aataccctgc tccatttcca gttgcactaa
    34561 agaattgaac ggtgatccag acggatcttt gccgacgcgt tgccagattt cctcttcgga
    34621 gtaatgacgc atggcgacaa cgagactcag cactttggaa atagactgaa tggaaaaacg
    34681 ttcttgcgcg tctccggcct gaaaaagctg tccgtcaacg gtacagatag caatccccaa
    34741 tcgggaaccg tctaccgtag ccagcgccgg aatataatcc gcgactttac cctgaccaat
    34801 gagcggccgc acttgccgca agatgttttc taaaattgca ttatccatgg cgactgccac
    34861 tttctactcc tggaccgcag gtctgaaaag acctgcgagt atatcagagc tgaatatata
    34921 gcgtcagacc cggtctttcc acaccgtctg gatattacag aattcgtgta agccaaaatg
    34981 ggaaagctca cgaccaaagc cactcttctt cacaccacca aaggcgactc gcgcgtcgct
    35041 ggcacagtaa ccattgataa acaccccacc gcattccaga cgtgccgcca tctgtctggc
    35101 ccgggtttcg tcggtggtga aaatggtcgc tgaaaggccg aactcgctat tattagccag
    35161 ttccagtgca tgttctgcat ctttcgcaat ggtgattgcc gcaacggggc caaacatttc
    35221 ttcccgaaac gcggtcattt ctggggtaac attcgccaga accgttggcg gatagtagtt
    35281 acctgcccca gccatctttt ccccgcccag taacaaacgc gcaccctgcg ccagggtttt
    35341 ctccacctga tgatgcagct catcacgtaa atcaaaacga gccattggtc cgagagtgtt
    35401 ctcttcgtca cggggatcgc ccattttcaa ggctgccgca gctgccacaa aacgttcggt
    35461 aaatgccgaa gcaattccct cttcgatgat aaagcgtttt gccgctgcac aaacctgtcc
    35521 ggtattctga taacgtccgg ctaccgccgc tttaaccgcc agttccagat cggcatcgtt
    35581 aagcacaata aatggatccg aaccgcccag ttccagtacg cattttttca gtgccgctcc
    35641 agcctgtgcg ccaatagccg ctcccgcacg aacacttccg gtcaccgtga cagcagcaat
    35701 gcgcgagtct ttaatcatct gactgacgcc gtcgttgtcg gcattcagcc agccatatac
    35761 gccttgtggg atacccgcat ctttaaacac ctgggcaatg agctgtgcac agcccatcac
    35821 attcggcgca tgtttaagta agtagccgtt acctgcaaga atgatgggaa ccgcgccacg
    35881 catcacctgc cataacggaa aattccacgg cataatcgcc agaattgtcc ccaacggtcg
    35941 atactcaata actgcctgct gattttccac cagcgtaggt tccgccttca gcattgccgg
    36001 accatgttct gcataccagt cacacaaatt cgccgatttc gccacttcag cgcgcgcctg
    36061 gttgattggt ttgcccattt cacgggtgat catttgcgcc atttcttcgc tacggacacg
    36121 cagagcctta ccgatatcac gcagtttttc agcacgataa tctatatttg tctcgcgcca
    36181 gtcgcgaaag cctgctgccg ccagctgaag tgcgttttcg atatcatcag cgccagccca
    36241 cggcagcaca gaaagttgtt caccggtggc aggatttatc gaaattgcat gagttgccgg
    36301 agtaatggtc atcggggtat ctcctttatg ggttatggta aaaagatacg cagatttact
    36361 cttgcttgaa aatgaataat attaagtcac ttattcacga attgagaatg ccatggatct
    36421 gacccaactg gagatgttca acgcggttgc cgaagctggc agcattaccc aggctgcagc
    36481 aaaagtgcat cgcgtgccgt cgaatctcac tacccgtcta cgccagctgg aaacagaact
    36541 ggggattgat ctgtttattc gcgagaatca gcgtttacgt ctctcacctg ccgggcataa
    36601 ctttttacgc tatagccaac aaattctcgc gttagtggat gaagcgcgga gcgttgtcgc
    36661 tggcgatgag ccgcaaggtt tattttctct tggttcgctg gaaagcaccg ctgcggtgcg
    36721 cattccggtc acgctggcgg aatttaaccg tcgttatccc aaaattcagt tttcactttc
    36781 caccggccct tccggcacta tgctggaagg ggtgctggag ggaaaactga atgcagcgtt
    36841 tattgatggg cccattaacc atactgccat tgacgggata ccggtgtacc gcgaggagct
    36901 gatgatcgtc acgccacaag gacatgcgcc agtaacccgc gccagtcagg taaatggcag
    36961 taacatttat gccttccgcg ccaattgttc gtatcgtcgc cacttcgaga gctggtttca
    37021 tgctgatggt gccgctccgg gaactattca cgagatggag tcgtatcacg ggatgttggc
    37081 ctgtgtggtc gcaggagcag gtattgcgct tattccgcgc tctatgctgg aaagtatgcc
    37141 ggggcatcac caggttgaag cgtggccgtt agctgagcaa tggcgttggt taacaacctg
    37201 gctggtctgg cgtcgcggtg cgaaaacgcg tccgctcgag gcatttattc aactgctgga
    37261 tgtgcctgac tcggcaaaac agggttacca atgagctatt tttgatagtt cttgcggcta
    37321 atatgctcta tatagtgatg ttccgatgac ttatgactat atggagcaaa tatggttacg
    37381 ccagtaagca tcagcaatta catatctctt cctgatgatt ttcccgtacg taatattgca
    37441 ccgcaggtaa aagaagtttt aaaggatttc attgatgcac ttagtacaat aatatgtgat
    37501 gaagaatggc gcacgtcttt aaacatcaac tcagccacaa aaaagatatt taataacctt
    37561 gacaacttat catatattca gaggacttct tttcggggta acgacacgct atacaatgaa
    37621 aaggttcagt ttaaactcac ttatcccgtc aagaatggaa gacacaaaga aaatattgaa
    37681 tttcaggtag taataaattt aagccctatt tatttagata atttccgcca tgatggggaa
    37741 attaatattt tttgcgctcc caacccaaag cctgtcacta tggggcgcgt atttcagacc
    37801 ggcgtcgagc gtgtgctctt tctttttctg aatgacttta tagaacaatt tccaataatc
    37861 aaccctggcg ttcctatcaa aagagcgcat acgccacata ttgaacccct gcatccggat
    37921 caccataccg ctgcagatta catacgccag tttgatttgc ttgtcctgaa ttttatctct
    37981 cgcggtaatt ttgtcatact cccccgatta tggaataact ctgaggttca cagatggttc
    38041 gggaataaag aacctaactt gctcaccgct attctcgaca taacggacag cgaattaaaa
    38101 gaggatttat tgcaaagtct gatggattca ctaggttcta acaaacatgt actacccgaa
    38161 gtctgcatct gctttttatc ccttttagca gaacaagatt ttccacattt tcaggagttg
    38221 tttttatttt tcgccaatat gttattgcac tatcaccaat ttatgaatcc caatgaaagt
    38281 gatttgattg acgtgttgat gccagcatca ttaagtgatg ataaaattat caaacatatg
    38341 gcgcgcaaga ccctcaaact ttttgtaaaa aatgaaacgc cgccaaaagt tactcatgaa
    38401 gacctggtga aaaacaggcc tcgctcccct gtcagaccac ctatacccgc aactgccaaa
    38461 acgccagacc tccctgaacg tcattaaacc gtgatgtgaa cgactctctg acgcgtgaaa
    38521 gaatcagcgt cagagaaacg gaaaacgcga tccagatcac aaatgcattg tattcacatc
    38581 attaaccgtt ttaagatcat ttcatcactt tttcgcaact cacccgataa tctgttatga
    38641 caacaaacac tgtttcccgc aaagtggcgt ggctacgggt cgttacgctg gcagtcgccg
    38701 ccttcatctt caacaccacc gaatttgtcc cagttggcct gctctctgac attgcgcaaa
    38761 gttttcacat gcaaaccgct caggtcggca ttatgttgac catttacgca tgggtagtag
    38821 cgctaatgtc attgcctttt atgttaatga ccagccaggt tgaacggcgc aaattactga
    38881 tctgcctgtt tgtggtgttt attgccagcc acgtactgtc gtttttatcg tggagtttta
    38941 ccgttctggt gatcagtcgc attggtgtgg cttttgcaca tgcgattttc tggtcgatta
    39001 cggcgtctct ggcgatccgt atggctccgg ccgggaagcg agcacaggca ttgagtttaa
    39061 ttgccaccgg tactgcactg gcgatggtct taggtttacc tctcgggcgc attgtggggc
    39121 agtatttcgg ttggcgaatg accttcttcg cgattggtat cggggcgctt atcacccttt
    39181 tgtgcctgat taagttactt cccttactgc ccagtgagca ttccggttcg ctgaaaagcc
    39241 tcccgctatt attccgccgc ccggcattga tgagcattta tttgttaact gtggtagttg
    39301 tcaccgccca ttacacggca tacagctata tcgagccttt tgtgcaaaac attgcgggat
    39361 tcagcgccaa ctttgccacg gcattactgt tattactcgg tggtgcgggc attattggca
    39421 gcgtgatttt cggtaaactg ggtaatcagt atgcgtctac gctggtaagt acggcgattg
    39481 cgctgttgct ggtgtgcctg gcactgctgc tacctgcggc gaacagtgaa atacacctcg
    39541 gggtgctgag tattttctgg gggatcgcga tgatgatcat cgggcttggt atgcaggtta
    39601 aagtgctggc gctggcacca gatgctaccg acgtcgcgat ggcgctattc tccgggatat
    39661 ttaatattgg aatcggcgcg ggtgcgttgg taggtaatca ggtcagtctg cactggtcaa
    39721 tgtcgatgat tggttatgtg ggcgcggtgc ctgcttttgc cgcgttaata tggtcaatca
    39781 tcattttccg ccgctggcca gtgacactcg aagaacagac gcaatagttg aaaggcccat
    39841 tcgggccttt tttagtggta cgttttaatg atttccagga tgccgttaat aataaactgc
    39901 acccccatac ataccagcag gaatcccatc agacgggaga tcgcttcaat gcccccctta
    39961 cccaccagcc gcataattgc gccagagctg cgtagacttc cccacaaaat aaccgccacc
    40021 aggaaaaaga tcagcggcgg cgcaaccatc agtacccaat cagcaaaggt tgaactctga
    40081 cgcactgtgg aggccgagct aataatcatc gctatggttc ccggacccgc ggtacttggc
    40141 attgccagtg gtacaaaagc aatattggcg ctgggttcat cttccagctc tttcgacttg
    40201 cttttcgctt ccggtgaatc aatcgctttc tgttgcggaa agagcatccg aaaaccgata
    40261 aacgcgacga ttaagccgcc tgcaattcgc agaccgggaa tcgaaatgcc aaatgtatcc
    40321 atcaccagtt gcccggcgta atacgccacc atcatgatgg caaatacgta caccgaggcc
    40381 atcaacgact gacgattacg ttcagcactg ttcatgttgc ctgccaggcc aagaaataac
    40441 gcgacagttg ttaatgggtt agctaacggt agtaacacca ccagccccag gcctattgct
    40501 ttaaacaaat ctaacattgg tggttgttat cctgtgtatc tggtttatca gcgaaaagta
    40561 taaggggtaa acaacgataa agtgtcactc tttagctaac cttgcatcgc attgaccaaa
    40621 acttgaaccg atttagcaaa acgtggcatc ggtcaattca ttcatttgac ttatacttgc
    40681 ctgggcaata ttatcccctg caactaatta cttgccaggg caactaatgt gaaaagtacc
    40741 agcgatctgt tcaatgaaat tattccattg ggtcgcttaa tccatatggt taatcagaag
    40801 aaagatcgcc tgcttaacga gtatctgtct ccgctggata ttaccgcggc acagtttaag
    40861 gtgctctgct ctatccgctg cgcggcgtgt attactccgg ttgaactgaa aaaggtattg
    40921 tcggtcgacc tgggggcgct gacccgtatg ctggatcgcc tggtctgtaa aggctgggtg
    40981 gaaaggttgc cgaacccgaa tgacaagcgc ggcgtactgg taaaacttac caccagcggc
    41041 gcggcaatat gtgaacaatg ccatcaatta gttggccagg acctgcatca agaattaaca
    41101 aaaaacctga cggcggacga agtggcaaca cttgagcatt tgcttaagaa agtcctgccg
    41161 taaacaaaaa aagaggtatg acgatgtcca gacgcaatac tgacgctatt accattcata
    41221 gcattttgga ctggatcgag gacaacctgg aatcgccact gtcactggag aaagtgtcag
    41281 agcgttcggg ttactccaaa tggcacctgc aacggatgtt taaaaaagaa accggtcatt
    41341 cattaggcca atacatccgc agccgtaaga tgacggaaat cgcgcaaaag ctgaaggaaa
    41401 gtaacgagcc gatactctat ctggcagaac gatatggctt cgagtcgcaa caaactctga
    41461 cccgaacctt caaaaattac tttgatgttc cgccacataa ataccggatg accaatatgc
    41521 agggcgaatc gcgcttttta catccattaa atcattacaa cagctagttg ataacgtgac
    41581 aacgtcactg aggcaatcat gaaaccactt ttatccgcaa ttgcagctgc gcttattctc
    41641 ttttccacgc agggcattgc ggaacaaacc acgcagccgg ttgttacttc ctgtgccaat
    41701 gtcgtggttg ttcccccatc gcaggaacaa ccaccgtttg atttaaatca catgggtact
    41761 ggcagtgata agtcggatgc gctcggcgtg ccctattata atcaacacgc tatgtagtta
    41821 ttgtcaggct ctgatcacag agcctgacat ctccgtaata tctttttgaa gtctcaaata
    41881 ttttcgccat tgttggcgat gagatcccga taccagtaaa acgacgcttt acgtatcctt
    41941 tccagcgttc ccttcccttc gttgtcttta tcgacataaa tcattccgta gcgttttttc
    42001 atttcccctg ttccggcaga aaccaggtca atgcagcccc acggtgtgta gccaattaag
    42061 tcaacaccat cttcaacaac ggcttttttc atttcccgaa tatgggaagc aaagtactca
    42121 atgcgatagt gatcgttcac cgtgccgtca gcttgtctct ggtcaactgc acccaatcca
    42181 ttttcgacaa taaacagcgg caactggaaa tgatcccaga accagttgag tgaataacgt
    42241 agccctgccg gatcaatttg ccatccccat tcggatgtat cgatataagg gttgctgacc
    42301 aaatctcgtg gttcaacata attcagttgc gggttatctg gcgaaaattg cgtagtaaaa
    42361 gacatgtagt agctaaaacc gataaagtcg acacaacctc tggcaagaat cgtgttatct
    42421 tctggtgtga tatcgaggtt gaatcctttc ctggcaaagt aattcagcat atgttgcgga
    42481 taatatccac gcgcatgaac atcagtaaac cagtaacgac gatgcatcgc tttcgtggcc
    42541 atcatcatat cgttgggtgc acacgtcaga ggatagatgg ggcacatggc gatcatacag
    42601 ccgatattaa attcaggatt gatcgatttt ccagtctgta ccgccagggc actggcaact
    42661 aactcgtaat gtaccgcctg gtacattatt tgttcgcgct cattgatatc ttcctctggc
    42721 gaatacaaga taccggaatt agtaaatgga cacaggcttt cgctgaaatt cacctgatta
    42781 ttgatttcgt taaacgtcat ccagtacttt actttttctt tatagcgcgt gaagaccgtt
    42841 gatgcgaagc ggatgaaaaa gtcgatcagt ttacggtgtc gccagccacc atattttgtc
    42901 accagatgat aaggcatctc aaaatgcgaa agcgtcacca caggctccat tccctgcttc
    42961 aggcattcat cgaacagatc atcataaaat tgtaaaccct cttcattcgg ctcctgttcg
    43021 tcaccttgcg gaaagattcg tgtccaggca atggaagttc gaaagcattt gaatcccatc
    43081 tcggcaaata actgaatatc tgttttatag cgatgataaa aatcaattgc ttcatgattg
    43141 ggataattaa gcccgtcgat aatgccttct gtcacttcac gcggcacccc gtgagcgcca
    43201 gcagtcatca catcagcgat actgatgcct tttcctcctt cattccagcc accttccaac
    43261 tgatgcgcgg ctaccgcgcc accccataaa aaaccttttt taaatcctga catacacaac
    43321 tccttaacta aaatagatta ccaccagact tccgcctgga tgccggccat aaattcgtct
    43381 ttgctgttct cattaaactt aaaattagat aattcgttat ccagaatatt gatatacgtg
    43441 ccatagaagc gaatttctgg tcgtgaacct aaaatgcttt cacccacttt caatgcatgc
    43501 cagagtgtcg ttttataagc cgattcattt agtgttacac cctgctgtgc tttgttctgt
    43561 tgcttaaacc agcctaattc aagccccgtc tgattccatg tattccagat ccaggccggt
    43621 cttattacgg tgcgaatact gtcaaaatca ctatgagcgc cactttcata actataaaca
    43681 tcttcgccat gagaatagac aagcgcgtta gccataataa tattgtcagt aagatacatc
    43741 tcgccctgag agattaaacg ccaggcgatc ccattggtat ggtcaccata gtaatagcga
    43801 ccatgcgcca tcgtgttact ggcatctgag aaactggcaa aactgctggc ataggaatta
    43861 ttggcaacct gtaacgtaaa ttcattaaac gtatcgcgtt gcagtttttg ccgtaaaaca
    43921 gaagtcagca tccagctatc tttcatttca aaataactgt cgtcattttc attatcttgt
    43981 tgttgatccg ttttattagg tgcggaatat ttagccatta atgataatgt tgcatcatcc
    44041 cataacggga tattgcgata acgaacatcc acagaattag tattcatctg actggtacgc
    44101 gtaaaatcac gggagtaaac atcgacatca tctcggctta aggacatatc aaacagccct
    44161 acaccaagtg cccagttttc aatccccaca cccgcagcga catccgtggt taaggttttc
    44221 cagtccagca tttggatctc atattgcggg agtttatgtt tgcctaccca gaagtctgcc
    44281 tctggcgcga agggtaaaaa acctcgcgtt gtcagataaa tatcactgaa ctgcatgata
    44341 ttttcattgg cactgtcacc aaaccaggca tcgttatact gctcacctac gttgccatca
    44401 taggtaacga ccgcatttgc cgttttaccg tcctgattat aaacacgctg atttaaggtt
    44461 aagtcaaacc aaccgctcat ctcgttacca aaacgtccca gagaacccgc tgcataagtt
    44521 tgtggtgagc catgatttcc ggtaccccaa cctgagcgaa agtaccccct gatagctgaa
    44581 cccaatatca tcttttatat atttactgat atctttcagg gtcacgcttt cgatagtggt
    44641 tttatcgcct ttactttcaa taacggcaat ttgctgcgat ccatttatag tatgtgctgc
    44701 ggcagacgag ttctgttcac cgttttcatt tttgatgtta tcagcaacgg gagatggagc
    44761 tgatatttcg gcaagcgagg ccgattgata tttattttct gtgatgcttt tttgtaatgt
    44821 cgaaagtcgg aatttatata ctcctagttc attctgtgtt gctctcagct cttgtttatt
    44881 ttgcgacaat tcattttcaa gcagttcaag gcgctgctct atggttaatt ttgttgccat
    44941 cgcgttcaat ggaaacaatg ctgtaataat agaaaaagac aaaaggctga cgttaagcgt
    45001 ctttatattc atgacttatc cttaagagtg taataatttc ctccagctat cgcactggag
    45061 gaaaaaatca ggcatggagc tcttttctta atgaaatgat ttccctggct aaatcctggc
    45121 ataatattgc attcatcaaa tgatcctgtg catgtaccag aatgagatta acttttattt
    45181 tgccacagcc ttcatcctga ctaattaacg cggtctgaat cttatgcgcc tgaagacagg
    45241 cggattcaga actggccatt agctgagttg cgccctgcca gtctttttgc ccggcacaac
    45301 gtatggcttc catcgcatca gaacgggcct gtcctgcgtt gaccagtaac tccataacaa
    45361 gttcttcatc tgcaaacatc aacatctcct tcctgtgtaa atggcattat ccgttagttg
    45421 ttaagttatt gaccttgttg gcggcaataa caaaaggtaa atagatcatt gtcgccacac
    45481 ctaaacataa aaaacccacc agtaacgcca gccagtttcc cccggttccg aggaaagcaa
    45541 ttaaaggtcc gggcgtagtc cagggcacgg cataagcaac cggtggaata atttccatcg
    45601 aaacaaagaa gtaaccaatg gcacagttaa caataggaac cataataaac ggcaccatca
    45661 tgatggggtt aagtacaata ggaaggccga acattatcgg ttcattgata ttgaaaatac
    45721 cggggataaa tgacattttt gccagatcac ggtattccgc acgcttagaa gcgataaaaa
    45781 tagccaacaa taaccccaac gtcatacctg aaccgccgca gttggcgaat gcatcattaa
    45841 tagaagtcca ggtaatcgga tatggcgcgc cccaggtagt gccgtgttga gcggcccagg
    45901 agagattctc cagattagcc tcagaaaaaa cagtttctcg aattgccgag gtggtattag
    45961 gtccgtggat ccccagcacc cacaggaaat tacccacagc ccccaggata attaccgcaa
    46021 agatattcgt tcccatatgt ttgagcggcg tctggattaa tgtgtaaatg aggtcgttta
    46081 atcctgccgg ggatatcagt gtcaggcaat aattaagtgc ggaaaagaac accatgacaa
    46141 aaaatattgg caataaaact ttaaatgaac gcgctaccgc aggaggtaca gctgccggca
    46201 tcgtgatggt gattttgggg tttcgagcta agcgacaaaa tatttcactg gtgatcagtg
    46261 caacgataac agcaacaaat aacccctgcg cgccgagata tttggtcgtc aggaatgctt
    46321 gcccatctat catctgatat ggcgtatata caataaaaaa tgctcctatt gccgttaatc
    46381 cgcataaaag atcatcttgc tcatagctaa tcgccatatt cctggcgacc aggaaagcaa
    46441 tcataattga catgatattt acagaaccat tcattaccgg agtaaaaata gcttgtgctt
    46501 tttcaaggtt ggggaaaacg ctgttcagat gcagcagccc ggcaataaag ccgtcgggcg
    46561 ataatatggc aaagttaatt aatataatta atgagcttgc catgataatt ggaaacgtaa
    46621 aaacgaatcc atctctcacc gctgcaacat gtttttgtga gtttaacttg atagcaactg
    46681 gaacaagaaa acgttccatt ccacgttcga atgaggccat taatcccatt ttttatacct
    46741 gttgttttgg atatttatac atcccaataa agcatgtatg ccatatccat tttatgatgt
    46801 gtgaaaagtt agttcaggta atagtttaat aaaattagat tcttactgtt gcagtagttt
    46861 catcgcaaat tcaagaactg cctgtccgtt catggtgcca tagtctttca tctcgataac
    46921 agctacaggc attttcccct gtgcaacagc ttcaatctct ggtttctgaa agcgaacttg
    46981 cgggccaagt aaaacaacgt caacttcgtt atttttaatt ttccctttag cctctgcaat
    47041 cgccaatgcg gaaatattaa cttcaagtga aatagcgtta gcatgatcaa tcatacgttt
    47101 aaccagcata ctggttgaca tgcccgcagc gcaaactaac aatatctttt tcatattttc
    47161 ccttactggt atataacaga ttacattgtg taaatatcat atcccgcagt agctatacgt
    47221 cctggtacat tgatcacagt aatacagcac taaagttgtt caacatcagg gtgataatta
    47281 aacgactcgg ccataggata aaccaatcag gactcaactc cttgttttca atggctctta
    47341 aaaggtgcaa aagtgatttt tcaaaagatt gcccgcttac ttaaatccga gatcaatggc
    47401 tattcatggc atgttggtga tttgttgccg tcagaagctg aactcgctgt tcgttataat
    47461 gtttcgcgta ataccctgcg taaggcatta tccttgctgg aaggcgaagg aattattcat
    47521 agaaagcatg gttcaggaac atacattcaa aaaaagaatt ttgttgcaca cattgatcac
    47581 atgaacagtt tcagtgaaat tgcgcataaa agcggcaaag aggcagggag tcagattatg
    47641 aaatttgaag tgcaggatgc ttctccccct attgcgactg agctgaattt agtgactggt
    47701 gagcaggttt attacataaa gagactgcga ttcattgagg ataatgcagc acaattagaa
    47761 gaaacgtgga tgtcagtggc acgttttcct gatttaaccg tagcacatat gcaaaaatcc
    47821 aaattttcgt atattgagaa cgaatgcggg atcaaaatca ttggcacctt tgaaactttc
    47881 tccccgactt ttcctacccc agaaatcgcc agtattttac ggatcagccc acgggatccc
    47941 atacttaaaa ttcagaccca ggctgtggac agtaactcta ttccgctgga ttattcgtta
    48001 ctttacagca atattttcga gttccaggta aagtactttt tcccgcgata aaagcagacg
    48061 cacagagtgc tggcatcaca ttctgtgcgt atggtttagc ctctaacctt taccgcttta
    48121 cgccaccgca agccaaatac attgatatac agcccggtca taatgagcac cgcaccgaaa
    48181 aattgcaggc ccgttaagcg ttcatccagc aatagtgccg cacttgccag tccaactaca
    48241 ggcaccagta acgataacgg tgcaacccgc caggtttcat agcgtcccag taacgtcccc
    48301 cagatcccat aaccaacaat tgtcgccaca aacgccagat acatcagaga caagatggtg
    48361 gtcatatcga tagtaaccag actgtgaatc atggttgcgg aaccatcgag aatcagcgag
    48421 gcaacaaaga agggaatgat tgggattaat gcgctccaga ttaccagcga catcaccgcc
    48481 ggacgcgttg cgtgcgacat gatcttttta ttgaagatgt tgccacacgc ccaactaaat
    48541 gctgccgcca gggtcaacat aaagccgagc atcgccacat gctgaccgtt cagactatct
    48601 tcgattaaca ccagtacgcc aaaaatcgct aaggcgatcc ccgccaattg tttgccatgc
    48661 agtcgctccc cgaaagtaaa cgcgccaagc acgatagtaa aaaacgcctg tgcctgtaac
    48721 accagcgaag ccagtccagc aggcatacca aagttaatgg cgcagaacag aaaggcaaac
    48781 tgcgcaaaac tgatggttaa tccatacccc agcagcaaat tcagtggtac tttcggtcgt
    48841 gcgacaaaaa agatagccgg aaaagcaacc agcataaagc gcaaaccggc caacatcagc
    48901 ggtggcatgt tatgaagccc cactttgatg accacaaaat ttagccccca tacgaccact
    48961 accagtagcg ccaacacccc atcttttcgc gacattctac cgcctctgaa tttcatcttt
    49021 tgtaagcaat caacttagct gaatttactt ttctttaaca gttgattcgt tagtcgccga
    49081 ttacgacggc attaatgcgc aaataagtcg ctatacttcg gatttttgcc atgctattcc
    49141 tttacatctc taaaacaaaa cataacgaaa cgcactgccg gacagacaaa tgaacttatc
    49201 cctacgacgc tctaccagcg cccttcttgc ctcgtcgttg ttattaacca tcggacgcgg
    49261 cgctacgctg ccgtttatga ccatttactt gagtcgccag tacagcctga gtgtcgatct
    49321 aatcggttat gcgatgacaa ttgcgctcac tattggcgtc gtttttagcc tcggttttgg
    49381 tatcctggcg gataagttcg acaagaaacg ctatatgtta ctggcaatta ccgccttcgc
    49441 cagcggtttt attgccattc ctttagtgaa taacgtgacg ctggtggtgc tcttttttgc
    49501 ccttattaac tgcgcctatt ccgtttttgc taccgtgttg aaagcctggt ttgccgacaa
    49561 tctttcgtcc accagcaaaa cgaaaatctt ctcaatcaac tacaccatgc tcaacattgg
    49621 ctggaccatc ggtccgccgc tcggcacgtt gttggtgatg cagagcatca atctgccctt
    49681 ttggctggca gctatctgtt ccgcgtttcc catgcttttc attcaaattt gggtaaagcg
    49741 cagcgagaaa atcatcgcca cggaaacagg cagagcctgg tcgccgaaag ttttattaca
    49801 agataaagca ctgttgtggt ttacctgctc tggttttctg gcttcctttg ttagcggcgc
    49861 atttgcctcg tgcatttcac aatatgtgat ggtgattgct gatggggatt ttgccgaaaa
    49921 ggtggtcgcg gttgttcttc cggtgaatgc tgccatggta gttacgttgc aatattccgt
    49981 gggccgccgc cttaacccgg ctaacatccg cgcgctgatg acagcaggca ccctctgttt
    50041 cgtcatcggg ctggtcggtt ttattttttc cggcaacagc ctgctaatgt ggggtatgtc
    50101 agctgcggta tttactgtcg gtgaaatcat ttatgcgccg ggcgagtata tgttgattga
    50161 ccatattgcg ccgccaggaa tgaaagccag ctatttttcc gcccagtctt taggctggct
    50221 tggtgctgcg attaacccat tagtgagtgg catagtactt accagtctgc cgcctttctc
    50281 gctgtttatc atcttagcgt tggtgatcgt tgctgcgtgg gtgctgatgt taaaaggaat
    50341 tcgtgcaaga ccgtgggggc agcccgcgct ttgttgattt aagtcgaaca caataaaaat
    50401 ttaattcagc cttcgtttag gttacctctg ctaatatctt tctcattgag atgaaaatta
    50461 aggtaagcga ggaaacacac cacaccataa acggaggcaa ataatgctgg gtaatatgaa
    50521 tgtttttatg gccgtactgg gaataatttt attttctggt tttctggccg cgtatttcag
    50581 ccacaaatgg gatgactaat gaacggagat aatccctcac ctaaccggcc ccttgttaca
    50641 actgtgtaca aggggcctga tttttatgac ggcgaaaaaa aaccgccagt aaaccggcgg
    50701 tgaatgcttg catggataga tttgtgtttt gcttttacgc taacaggcat tttcctgcac
    50761 tgataacgga tcgttgacac agtagcatca gttttctcaa tgaatgttaa acggagctta
    50821 aactcggtga atcacgtttt gttcgtcaat aaacatacag cgatttcttc cggtttgctt
    50881 accctcatac attgcccggt ccgctcttcc aatgaccaca tccagaggct cttcaggaaa
    50941 tgcgcgactc acacctgctg tcacggtaat gttgatatgc ccttcagaat gtgtgatggc
    51001 atggttatcg actaactggc aaattctgat acctgcacga catgcttctt catcattatc
    51061 cgctttgaca ataatgataa attcttcgcc cccgtagcga taaaccgttt cgtaatcacg
    51121 cgtccaactg gctaagtaag ttgccagggt gcgtaatact acatcgccga ttaaatgccc
    51181 gtaggtatca ttaaccaatt taaatcggtc aatatccaac aacattaaat aaagattcag
    51241 aggctcagcg ttgcgtaact gatgatcgaa ggattcatca agaacccgac gacccggcaa
    51301 tcccgtcaaa acatccatat tgctacggat cgtcagtaaa taaattttgt aatcggttaa
    51361 tgccgcagta aaagaaagca acccttcctg aaaagcgtcg aaatgcgcgt cctgccagtg
    51421 attttcaaca atagccaaca ttaattcccg accacagtta tgcatatgtt gatgggcaga
    51481 atccattagc cgaacgtaag gtaattcatc gttatcgagt ggccccagat gatcaatcca
    51541 ccgaccaaac tggcacagtc cataagaatg gttatccgtt atttctggct tactggcatc
    51601 tctcgcgacc acgctgtgaa acatacttac cagccactgg tagtgggcat cgatagcctt
    51661 attgagattt aacaagatgg catcaatttc cgttgtcttc ttgatcattg ccactccttt
    51721 ttcacagttc cttgtgcgcg ctattctaac gggagaaaag caaaattacg tcaatatttt
    51781 catagaaatc cgaagttatg agtcatctct gagataacat tgtgatttga aacaaaatca
    51841 gcagataaaa aagtgtttaa ttctgtaaat taactctgca ttatcgcaat taaaaggatg
    51901 acaaatagca taacccaata ccctaatggc ccagtagttc aggccatcag gctaatttat
    51961 ttttatttct gcaaatgagt gacccgaacg acggccggag cgcttttctt atccagactt
    52021 ccgctaatgt tgatcatctg gtccggctga acttctcgtc catcaaagac tgccgcagga
    52081 ataacaacat taatttcacc gctcttatcg cgaaaaacgt aacggtcttc tcctttgtga
    52141 gaaatcaaat taccgcgcag tgaaaccgaa gcgccatcat gcatggtttt tgcgaaatca
    52201 acggtcattt tttttgcatc atcggttccg cgatagccat cttctattgc atgaggcggc
    52261 ggtggcgctg catcctgttt taatccgccc tggtcatctg ccaacgcata aggcatgaca
    52321 agaaaacttg ctaatacaat ggcctgaaat ttcatactga ctccttaatt gcgtttggtt
    52381 tgacttatta agtctggttg ctatttttat aattgccaaa taagaatact gccaattgtt
    52441 ataaggcatt taaaatcagg ctactagatg tcaaatattc agagaattta actcactaaa
    52501 gttaagaaga ttgaagagtc ttaaacatat ttcagaacaa tcagatttat aaatttgaaa
    52561 attattataa cggaagagta tacaaaaaaa gtaattttgc tggcgactgc gaaacggcat
    52621 tcgcattagc ggtgaagttt gccctcgccc agctgctgca attactgcta taacgaggct
    52681 ggtctggcga taaatccagg ccagccattg gtggtgttta tacgttcaag ccacgatgtt
    52741 gcagcatcgg cataatctga ggtgccttac cgcgccattg tcgatacagg cgttccagat
    52801 cctcgctgtt acctctggaa aggatcgcct cgcgaaaacg cagcccattt tcacgcgtta
    52861 atccgccctg ctcaacaaac cactgataac catcatcggc caacatttgc gtccacagat
    52921 aagcgtaata acccgcggca tatccgccac caaaaatatg ggcgaaataa ctgctgcgat
    52981 agcgtggcgg tatagcggga agatccatat tttccgccac cagcgcccgc aattcaaaat
    53041 catcgacatc ctgcattgct tcgttttctt ccaggcaatg ccagcgcata tcgagaagtg
    53101 cggcgctaag cagttcgctc atctcatacc ctttgttgaa caggctggca ttacgcattt
    53161 tctgttgcag ttcgtcaggc attgctgccc cgctctgata atgccgggcg tagcgagcga
    53221 atacctgcgg atgtgttgcc cagtgttcgt tgatttgcga cggaaattcg acaaaatcac
    53281 gcggcgtgtt ggtgccggaa agcgtggcat aacgctggcg ggcaaaaagg ccgtgcagcg
    53341 tatgaccaaa ttcatggaat aaggttatta catcatccca gagtaacaac gcaggctcac
    53401 cggcagcggg tttctgataa ttgcagacgt tataaattac cggatgcgtt tcattaagcg
    53461 ttgattgctc aacaaaattg cccatccatg caccgccgct ttttgaatca cgggcgaaga
    53521 aatcaccgta aaataacgcc agccccacgc cattatgatc aaaaatttcc cacacccgaa
    53581 cgtcaggatg gtagacagga atatcaaaac gttcgacaaa cttaataccg aagagctgat
    53641 tcgcggtcca gaatacacct tcattcaaca ccgtgtttaa ttcaaaatat ggcttgagct
    53701 gcgattcatc gagatcgaat ttctcacgtc ttacctgctc ggcataaaat gcccagtccc
    53761 acggctgcgc gctaaacccg ccctgctgct tatcaataat cgcctgtatg gaggctaact
    53821 catcgctcgc acgttgacgc gccgctggaa caatttcccg cataaagttg agcgctgctt
    53881 ctggcgtttt tgccatctga tcggcgattt tccatgcggc ataatgagga aaaccaagca
    53941 gtttcgcctg ctgcgcgcgg atctccacca gacgttgaat gatagcgcgg gtgtcattgg
    54001 catcattttt ttccgctcgc atccagcccg cagtaaacag tttttcacgc gtcgcgcgat
    54061 cgcacagttc agcaagcgct ggttgttggg tggtattcag cagcggaatc agccatttgt
    54121 tatacagacc tttctcgcga gccgcctctg ccgccagcac aatctcttgc tcactcattc
    54181 ctgccagctg cgcgatatcg ttcacaacca gaccgccgga tttatttgct gccagtaacc
    54241 gctggttaaa ctggctggtc agggtcgcag cttctgtatt cagtactttt aattttgctt
    54301 tatcagcttg cttaagtttg gctccggcaa ggacaaaacg ttgatgaatc acctccacca
    54361 ggcggatgga ttcactatca agccccaggg attcacggcg ctgccagaca gcatctaccc
    54421 gcgcgaataa ttccccgttc agatagatat cattcgcaag ttccgccagt tcagcggaaa
    54481 actgttcatc aagacgctgt aattcatcat tggtatgcgc cgcagtcatc gcaaaaaaga
    54541 cgctggtaac gcgggtaagt aattctccgc tttgttccag tgccagaata gtattgttga
    54601 aatcaggtgt ttgcgggtta agcgcgatgg cagcaatttc tgcccgcttt tgctgcattc
    54661 cctcatcgaa tgccgggcga tagtgatgat tggcaatttg atcaaaatgg ggagccagat
    54721 acggcagtgt gctttgcaca aggaaaggat tcattgttgt cattttcttc tcctgaacgc
    54781 gaggtgttcc atagcgtagg cttactgata acggagtgca atcttgcaaa ccagtattac
    54841 ccgctcttaa gcatcacgtg ctatgttatt gacacacaaa agcgttgagg aacagtgaga
    54901 tgatcgtttt agtaactgga gcaacggcag gttttggtga atgcattact cgtcgtttta
    54961 ttcaacaagg gcataaagtt atcgccactg gccgtcgcca ggagcggttg caggagttaa
    55021 aagacgaact gggagataat ctgtatatcg cccaactgga cgttcgcaac cgcgccgcta
    55081 ttgaagagat gctggcatcg cttcctgccg agtggtgcaa tattgatatc ctggtaaata
    55141 atgccggtct ggcgttgggc atggagcctg cgcataaagc cagcgttgaa gactgggaaa
    55201 cgatgattga taccaacaac aaaggcctgg tatatatgac gcgcgccgtc ttaccgggta
    55261 tggttgaacg taattatggt catattatta acattggctc aacggcaggc agctggccgt
    55321 atgccggtgg taacgtttac ggtgcgacga aagcgtttgt tcgtcagttt agcctgaatc
    55381 tgcgtacgga cctgcatggt acggcggtgc gcgtcaccga catcgaaccg ggtctggtgg
    55441 gtggcaccga gttttccaat gtccgcttta aaggcgatga cggtaaagcg gaaaaaacct
    55501 atcaaaatac cgttgcattg acgccagaag atgtcagcga agccgtctgg tgggtgtcaa
    55561 cgctgcctgc tcacgtcaat atcaataccc tggaaatgat gccggttacc caaagctatg
    55621 ccggactgaa tgtccaccgt cagtaatttt tatacccggc gtaactgccg ggttattgct
    55681 tgtcacaaaa aagtggtaga ctcatgcagt taactcactc acaagcaaga acgaatgacc
    55741 gtcgaaacgc aacttaatcc cacacagcct gtcaatcagc agatttatcg tattcttcgt
    55801 cgcgacattg tccattgcct gattgctcca ggcacaccgt tgtcggaaaa agaagtttct
    55861 gttcgtttca atgtgtcacg ccagccggtt cgtgaagcct ttattaaact ggcggaaaac
    55921 ggcctgattc aaattcgtcc gcagcgtggc agctacgtca acaaaatttc catggcccag
    55981 gtgcgcaacg gcagttttat ccgtcaggcc attgagtgcg cggtggcgcg tcgggcggcg
    56041 agcatgatta ccgaaagcca gtgctaccaa ctggaacaaa atcttcacca gcaacgcatt
    56101 gccattgagc gcaagcaact ggatgatttt tttgaacttg atgacaactt ccatcaactc
    56161 ctgacccaga ttgccgactg tcaactggcg tgggatacca ttgagaacct gaaagcgacc
    56221 gttgatcgcg tgcgctatat gagtttcgac cacgtttctc caccagaaat gctgttacgc
    56281 cagcatcttg agattttctc tgccctgcaa aaacgtgatg gcgatgcggt agaacgtgca
    56341 atgacgcaac atttgcagga aatcagcgaa tccgtgcgcc agatccgtca ggaaaacagc
    56401 gactggttta gcgaagagta attcatttcc tctcatccca tccggggtga gagtttttcc
    56461 ctccgtcata tggctcatgc atgcatcaaa aaagatgtga gcttgatcaa aaacaaaaaa
    56521 tatttcactc gacaggagta tttatattgc gcccgttacg tgggcttcga ctgtaaatca
    56581 gaaaggagaa aacacctatg acgacctacg atcgtaaccg taacgcaatc accactggca
    56641 gccgtgttat ggttagcggc accggtcaca ctggcaagat cctgtcgatt gatactgaag
    56701 gtctgaccgc tgagcaaatc cgtcgcggaa aaaccgtagt tgttgaaggt tgtgaagaga
    56761 aactggcacc tctggacctg attcgtctcg gcatgaacta agcgtgtgaa tgccgccgat
    56821 ggcggcattg ctttttttac ttcacggaat atttcgccac ggtcgctttc gcaccatgcg
    56881 ctaataaaga caagtacgct tccgtcactt ttgcagtaaa caagctattg tctggcaaat
    56941 catcaccaaa gatcgcctta atcgccagca atgactggac gcgcgctttc ccttcggcac
    57001 tactttgtac agccttctga ataacaggta acagtggatc actgatttca atcggatttc
    57061 cctgttcatc aacaccaccg acatagcgca tccaacccgc gacgcccagc gccagcagat
    57121 cgaacttgct gtcatgcgcc agatgccagc gaacagaatc caacatccgc tgtggcaatt
    57181 tctggctgcc atccatcgca atctgccagg ttcggtgacg tagcgccggg ttgctatagc
    57241 gttcaattaa tcggttagcg taatcttgca aatcaacgcc ctgcactttc aacgtcggcg
    57301 cttgttcctg caacatcaag gcatacgctg catgacgata atgttcatct tccatgcagt
    57361 cattaatgtg ctgatatccg gcaagataac ccagatacgc caggaatgaa tgactgccgt
    57421 tgagcatgcg caacttcatc tcttcataag gcagcacatc actaaccagt tcggctcccg
    57481 ctttttccca ttccggacgt ccggcaacaa agttatcttc tattacccac tggcggaaag
    57541 gttcacaggc aacgccagca ggatcgcgca caccggtaag ttgttcgatt ttcgccagcg
    57601 tatcctctgt cactgcgggc acaatacggt ccaccattgt tgatgggaaa gtcacgtttt
    57661 cttcgatcca ttgtgccagt tttacatcaa cagcttgcgc gtaggaagtg acaacgtcac
    57721 gcataacatg
//
LOCUS       DAMZFT010000015        54210 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.015, whole genome shotgun sequence.
ACCESSION   DAMZFT010000015 DAMZFT010000000
VERSION     DAMZFT010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 54210)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 54210)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..54210
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.015"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(100..1233)
                     /gene="nagA"
                     /locus_tag="PN977_001045"
     CDS             complement(100..1233)
                     /gene="nagA"
                     /locus_tag="PN977_001045"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HDI6041104.1"
                     /translation="MTHVLRARRLLTEEGWLDDHQLRIADGVIAAIEPIPVGVTERDA
                     ELLCPAYIDTHVHGGAGVDVMDDAPDALDKLAMHKAREGVGSWLPTTVTAPLNTIHAA
                     LKRIALRCQRGGPGAQVLGSYLEGPYFTPQNKGAHPPELFRELEIAELDQLIAVSQHT
                     LRVVALAPEKEGALQAIRYLKQQNVRVMLGHSAATWQQTRAAFDAGADGLVHCYNGMT
                     GLHHREPGMVGAGLTDKRAWLELIADGHHVHPAAMSLCCCCAKERIVLITDAMQAAGM
                     PDGRYTLCGEEVQMHDGVVRTASGGLAGSTLSVDAAVRNMVELTGVTPAEAIHMASLH
                     PARMLGVDGVLGSLKPGKRASVVALDSGLHVQQIWIQGQLASF"
     gene            complement(1230..1664)
                     /locus_tag="PN977_001046"
     CDS             complement(1230..1664)
                     /locus_tag="PN977_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDI6041105.1"
                     /translation="MLSIILTGHGGFASGMEKAMKQILGEQSQFIAIDFPETSSTALL
                     TSQLEEAIVQLDCEDGIVFLTDLLGGTPFRVASTLAMQKPGCEVITGTNLQLLLEMVL
                     EREGLSGEEFRVQALECGHRGLTSLVDELGRCHEECPVEEGI"
     gene            complement(1682..2560)
                     /locus_tag="PN977_001047"
     CDS             complement(1682..2560)
                     /locus_tag="PN977_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019079236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HDI6041106.1"
                     /translation="MASNQTTLPNVSENEETLLTGVNENVYEDQSIGAELTKKDINRV
                     AWRSMLLQASFNYERMQASGWLYGLLPALKKIHTNKRDLARAMKGHMGFFNTHPFLVT
                     FVIGIILAMERSKQDVNSIQSTKIAVGAPLGGIGDAMFWLTLLPICGGIGASLALQGS
                     ILGAVVFIVLFNVVHLGLRFGLAHYAYRMGVAAIPLIKANTKKVGHAASIVGMTVIGA
                     LVATYVRLSTTLEITAGDAVVKLQADVIDKLMPAFLPLVYTLTMFWLVRRGWSPLRLI
                     AVTVVLGIVGKFCHFL"
     gene            complement(2550..3329)
                     /locus_tag="PN977_001048"
     CDS             complement(2550..3329)
                     /locus_tag="PN977_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HDI6041107.1"
                     /translation="MEISLLQAFALGIIAFIAGLDMFNGLTHMHRPVVLGPLVGLVLG
                     DLHTGILTGGTLELVWMGLAPLAGAQPPNVIIGTIVGTAFAITTGVKPDVAVGVAVPF
                     AVAVQMGITFLFSVMSGVMSRCDRMAENADTRGIERVNYLALLALGTFYFLCAFLPIY
                     FGAEHAKTIIDVLPQRLIDGLGVAGGIMPAIGFAVLLKIMMKNVYIPYFILGFVAAAW
                     LKLPVLAIAAAALAMALIDLLRKSPEPTQPAAQKEEFEDGI"
     gene            complement(3340..3813)
                     /gene="agaV"
                     /locus_tag="PN977_001049"
     CDS             complement(3340..3813)
                     /gene="agaV"
                     /locus_tag="PN977_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIB"
                     /protein_id="HDI6041108.1"
                     /translation="MPNIVLSRIDERLIHGQVGVQWVGFAGANLVLVANDEVAEDPVQ
                     QNLMEMVLAEGIAVRFWTLQKVIDNIHRAADRQKILLVCKTPADFLTLVKGGVPVNRI
                     NVGNMHYANGKQQIAKTVSVDAGDIAAFNDLKAAGVECFVQGVPTEPAVDLFKLL"
     gene            complement(3836..5116)
                     /gene="kbaZ"
                     /locus_tag="PN977_001050"
     CDS             complement(3836..5116)
                     /gene="kbaZ"
                     /locus_tag="PN977_001050"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit KbaZ"
                     /protein_id="HDI6041109.1"
                     /translation="MKHLTEMVRQHKEGKTNGIYAVCSAHPLVLEAAIRYASANQTPL
                     LIEATSNQVDQFGGYTGMTPADFRGFVCQLADSLNFPQDALILGGDHLGPNRWQNLPA
                     AQAMANADDLIKSYVAAGFKKIHLDCSMSCQDDPIPLTDDIVAERAARLAKVAEETCR
                     EHFGAADLEYVIGTEVPVPGGAHETLSELAVTTPDAARATLEAHRHAFEKQGLSAIWP
                     RIIALVVQPGVEFDHTNVIDYQPVKATALSQMVENYETLIFEAHSTDYQTPQSLRQLV
                     IDHFAILKVGPALTFALREALFSLAAIEEELVPAKACSGLRQVLENVMLDRPEYWQSH
                     YHGDGNARRLARGYSYSDRVRYYWPDSQIDDAFAHLVRNLADSPIPLPLISQYLPLQY
                     VKVRSGELQPTPRELIINHIQDILAQYHTACEGQ"
     gene            5365..6174
                     /gene="agaR"
                     /locus_tag="PN977_001051"
     CDS             5365..6174
                     /gene="agaR"
                     /locus_tag="PN977_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aga operon transcriptional regulator AgaR"
                     /protein_id="HDI6041110.1"
                     /translation="MSNTDASGEKRMTGTSERREQIIQRLRQQGSVQVNDLSALYGVS
                     TVTIRNDLAFLEKQGIAVRAYGGALICDSTTPSVEPSVEDKSALNTAMKRSVAKAAVE
                     LIQPGHRVILDSGTTTFEIARLMRKHTDVIAMTNGMNVANALLEAEGVELLMTGGHLR
                     RQSQSFYGDQAEQSLQNYHFDMLFLGVDAIDLERGVSTHNEDEARLNRRMCEVAERII
                     VVTDSSKFNRSSLHKIIDTQRIDMIIVDEGIPADSLEGLRKAGVEVILVGE"
     gene            complement(6229..6693)
                     /gene="yhaV"
                     /locus_tag="PN977_001052"
     CDS             complement(6229..6693)
                     /gene="yhaV"
                     /locus_tag="PN977_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system ribonuclease
                     toxin YhaV"
                     /protein_id="HDI6041111.1"
                     /translation="MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKA
                     ATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKNKDWSRVKFGAGRYRLFFRYSEK
                     EKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH"
     gene            complement(6693..7028)
                     /gene="prlF"
                     /locus_tag="PN977_001053"
     CDS             complement(6693..7028)
                     /gene="prlF"
                     /locus_tag="PN977_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin PrlF"
                     /protein_id="HDI6041112.1"
                     /translation="MPANARSNAVLTTESKVTIRGQTTIPAPVREALKLKPGLDSIHY
                     EILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNNPQKTRPFDTQQGKKLVAGMDVN
                     IDDEIGDDE"
     gene            complement(7177..8748)
                     /gene="garD"
                     /locus_tag="PN977_001054"
     CDS             complement(7177..8748)
                     /gene="garD"
                     /locus_tag="PN977_001054"
                     /EC_number="4.2.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate dehydratase"
                     /protein_id="HDI6041113.1"
                     /translation="MANIEIRQETPTAFYIKVHDTDNVAIIVNDNGLKAGTRFPDGLE
                     LIEHIPQGHKVALLDIPANGEIIRYGEVIGYAVRAIPRGSWIDESMVVLPEAPPLHTL
                     PLATKVPEPLPPLEGYTFEGYRNADGSVGTKNLLGITTSVHCVAGVVDYVVKIIERDL
                     LPKYPNVDGVVGLNHLYGCGVAINAPAAVVPIRTIHNISLNPNFGGEVMVIGLGCEKL
                     QPERLLTGTDDVQAIPVESASIVSLQDEKHVGFQSMVEDILQVAERHLHKLNQRQRET
                     CPASELVVGMQCGGSDAFSGVTANPAVGYASDLLVRCGATVMFSEVTEVRDAIHLLTP
                     RAVNEEVGKRLLEEMEWYDNYLNMGKTDRSANPSPGNKKGGLANVVEKALGSIAKSGK
                     SAIVEVLSPGQRPTKRGLIYAATPASDFVCGTQQVASGITVQVFTTGRGTPYGLMAVP
                     VIKMATRTELANRWFDLMDINAGTIATGEETIEEVGWKLFHFILDVASGKKKTFSDQW
                     GLHNQLAVFNPAPVT"
     gene            9123..10457
                     /gene="garP"
                     /locus_tag="PN977_001055"
                     /pseudo
     CDS             9123..10457
                     /gene="garP"
                     /locus_tag="PN977_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312032.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GarP"
     gene            10473..11243
                     /gene="garL"
                     /locus_tag="PN977_001056"
     CDS             10473..11243
                     /gene="garL"
                     /locus_tag="PN977_001056"
                     /EC_number="4.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxyglucarate aldolase"
                     /protein_id="HDI6041114.1"
                     /translation="MNNDVFPNKFKAALAAKQVQIGCWSALSNPISTEVLGLAGFDWL
                     VLDGEHAPNDISTFIPQLMALKGSASAPVVRVPTNEPVIIKRLLDIGFYNFLIPFVET
                     KEEAEQAVASTRYPPEGIRGVSVSHRANMFGTVADYFAQSNKNITILVQIESQQGVDN
                     VDAIAATEGVDGIFVGPSDLAAALGHLGNASHPDVQKAIQHIFNRASAHGKPSGILAP
                     VEADARRYLEWGATFVAVGSDLGVFRSATQKLADTFKK"
     gene            11273..12163
                     /gene="garR"
                     /locus_tag="PN977_001057"
     CDS             11273..12163
                     /gene="garR"
                     /locus_tag="PN977_001057"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HDI6041115.1"
                     /translation="MTMKVGFIGLGIMGKPMSKNLLKAGYSLVVADRNPEAIADVIAA
                     GAETASTAKAIAEQCDVIITMLPNSPHVKEVALGENGIIEGAKPGTVLIDMSSIAPLA
                     SREISEALKAKGIDMLDAPVSGGEPKAIDGTLSVMVGGDKAIFDKYYDLMKAMAGSVV
                     HTGEIGAGNVTKLANQVIVALNIAAMSEALTLATKAGVNPDLVYQAIRGGLAGSTVLD
                     AKAPMVMDRNFKPGFRIDLHIKDLANALDTSHGVGAQLPLTAAVMEMMQALRADGLGT
                     ADHSALACYYEKLAKVEVTR"
     gene            12260..13405
                     /gene="garK"
                     /locus_tag="PN977_001058"
     CDS             12260..13405
                     /gene="garK"
                     /locus_tag="PN977_001058"
                     /EC_number="2.7.1.165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312029.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate 2-kinase"
                     /protein_id="HDI6041116.1"
                     /translation="MKIVIAPDSYKESLSASEVAQAIEKGFREIFPDAQYVSVPVADG
                     GEGTVEAMIAATQGSERHAWVTGPLGEKVNASWGISGDGKTAFIEMAAASGLELVPAE
                     KRDPLVTTSRGTGELILQALESGATNIIIGIGGSATNDGGAGMVQALGAKLCDANGNE
                     IGFGGGSLNTLNDIDISGLDPRLKDCVIRVACDVTNPLVGDNGASRIFGPQKGASEAM
                     IVELDNNLSHYADVIKKALHVDVKDVPGAGAAGGMGAALMAFLGAELKSGIEIVTTAL
                     NLEEHIHDCTLVITGEGRIDSQSIHGKVPIGVANVAKKYHKPVIGIAGSLTNDVGVVH
                     QHGIDAVFSVLTSIGTLDEAFRGAYDNIYRASRNIAATLAIGMRNAG"
     gene            13438..13814
                     /gene="rnpB"
                     /locus_tag="PN977_001059"
     ncRNA           13438..13814
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="PN977_001059"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(14036..15223)
                     /locus_tag="PN977_001060"
     CDS             complement(14036..15223)
                     /locus_tag="PN977_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhaC family protein"
                     /protein_id="HDI6041117.1"
                     /translation="MFPVSSIGNDISSDLVRRKMNDLPESPIVNNLEALAPGIEKLKQ
                     TSIEMVTLLNALQPGGKCIITGDFQKELAYLQNVILYNDSSLRMDFFGYNARIIQRSD
                     NTCELTINEPLKNQEISTGNINVNCPLKDIYNEIRRLNVIFSCGTGGIVDLSSLDLRN
                     VDLELYDFTDKHMANTILNPFKLDDTDFTNANMFQVNFVSSKQNATISWDYLLKITPV
                     ITSINDMYSEEKIKLVESCLNALGDITEEQLKIMRLAIIESIPRATLTDELENELTKE
                     IYKSSSKINNYLNRIKLPEMKAFSLEKIDYYIDIIIKDYESVKENAYLIVPKINYNTD
                     LNIEDSSSEEFLSDNTLEKDENSHDNCFEVVKYNTYEAYNSEKQYFTREDYTYDYDLL
                     NAI"
     gene            complement(15245..15784)
                     /locus_tag="PN977_001061"
     CDS             complement(15245..15784)
                     /locus_tag="PN977_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312027.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6041118.1"
                     /translation="MKGFPIAHIFHPSIPPMHAVVNNHNRNIDYWTVKRKFAEIVSTN
                     DVNKIYSISNELRRVLSAITALNFYQGDIPSVMIRIQPENMSPFIIDISTGEHDDYII
                     QTLDVGTFAPFGEQCICSAVNKKELECIKETISKYCAKFTRKEAILTPPAHFNKTSIT
                     SDCWQILFFSPDHFNNYFY"
     gene            complement(16040..16384)
                     /gene="tdcR"
                     /locus_tag="PN977_001062"
     CDS             complement(16040..16384)
                     /gene="tdcR"
                     /locus_tag="PN977_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator TdcR"
                     /protein_id="HDI6041119.1"
                     /translation="MTGITIFYGDNIIRYVVNTKKGLRPYFKQLPDNYQAKFELNLMS
                     KFSNFIINKPFSAINNAARHIFSRYLLENKHLFYQYFKISNTGIDHLEQLINVNFLPH
                     DRTSFCECNRFP"
     gene            16573..17511
                     /gene="tdcA"
                     /locus_tag="PN977_001063"
     CDS             16573..17511
                     /gene="tdcA"
                     /locus_tag="PN977_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TdcA"
                     /protein_id="HDI6041120.1"
                     /translation="MSTILLPKTQHLVVFQEVIRSGSIGSAAKELGLTQPAVSKIIND
                     IEDYFGVELVVRKNTGVTLTPAGQLLLSRSESITREMKNMVNEISGMSSEAVVEVSFG
                     FPSLIGFTFMSGMINKFKEVFPKAQVSMYEAQLSSFLPAIRDGRLDFAIGTLSAEMKL
                     QDLHVEPLFESEFELVASKSRTCTGTTTLESLKNEQWVLPQTNMGYYSELLTTLQRNG
                     ISIENIVKTDSVVTIYNLVLNADFLTVIPCDMTSPFGSNQFITIPVEETLPVAQYAAV
                     WSKNYRIKKAASVLVELAKEYSSYNGCRRRQLIEVG"
     gene            17610..18599
                     /gene="tdcB"
                     /locus_tag="PN977_001064"
     CDS             17610..18599
                     /gene="tdcB"
                     /locus_tag="PN977_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional threonine ammonia-lyase/L-serine
                     ammonia-lyase TdcB"
                     /protein_id="HDI6041121.1"
                     /translation="MHITYDLPVAIDDIIEAKQRLAGRIYKTGMPRSNYFSERCKGEI
                     FLKFENMQRTGSFKIRGAFNKLSSLTDAEKRKGVVACSAGNHAQGVSLSCAMLGIDGK
                     VVMPKGAPKSKVAATCDYSAEVVLHGDNFNDTIAKVSEIVEMEGRIFIPPYDDPKVIA
                     GQGTIGLEIMEDLYDVDNVIVPIGGGGLIAGIAVAIKSINPTIRVIGVQSENVHGMAA
                     SFHSGEITTHRTTGTLADGCDVSRPGNLTYEIVRELVDDIVLVSEDEIRNSMIALIQR
                     NKVVTEGAGALACAALLSGKLDQYIQNRKTVSIISGGNIDLSRVSQITGFVDA"
     gene            18621..19952
                     /gene="tdcC"
                     /locus_tag="PN977_001065"
     CDS             18621..19952
                     /gene="tdcC"
                     /locus_tag="PN977_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine transporter TdcC"
                     /protein_id="HDI6041122.1"
                     /translation="MSTSDSIVSSQTKQSSWRKSDTTWTLGLFGTAIGAGVLFFPIRA
                     GFGGLIPILLMLVLAYPIAFYCHRALARLCLSGSNPSGNITETVEEHFGKTGGVVITF
                     LYFFAICPLLWIYGVTITNTFMTFWENQLGFAPLNRGFVALFLLLLMAFVIWFGKDLM
                     VKVMSYLVWPFIASLVLISLSLIPYWNSAVIDQVDLGSLSLTGHDGILITVWLGISIM
                     VFSFNFSPIVSSFVVSKREEYEKDFGRDFTERKCSQIISRASMLMVAVVMFFAFSCLF
                     TLSPANMAEAKAQNIPVLSYLANHFASMTGTKTTFAITLEYAASIIALVAIFKSFFGH
                     YLGTLEGLNGLILKFGYKGDKTKVSLGKLNTLSMIFIMGSTWVVAYANPNILDLIEAM
                     GAPIIASLLCLLPMYAIRKAPSLAKYRGRLDNVFVTVIGLLTILNIVYKLF"
     gene            19978..21186
                     /gene="tdcD"
                     /locus_tag="PN977_001066"
     CDS             19978..21186
                     /gene="tdcD"
                     /locus_tag="PN977_001066"
                     /EC_number="2.7.2.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionate kinase"
                     /protein_id="HDI6041123.1"
                     /translation="MNEFPVVLVINCGSSSIKFSVLDASDCEVLMSGIADGINSENAF
                     LSVNGGEPAPLAHHSYEGALKAIAFELEKRNLNDSVALIGHRIAHGGSIFTESAIITD
                     EVIDNIRRVSPLAPLHNYANLSGIESAQQLFPGVTQVAVFDTSFHQTMAPEAYLYGLP
                     WKYYEELGVRRYGFHGTSHRYVSQRAHSLLNLAEDDSGLVVAHLGNGASICAVRNGQS
                     VDTSMGMTPLEGLMMGTRSGDVDFGAMSWVASQTNQSLGDLERVVNKESGLLGISGLS
                     SDLRVLEKAWHEGHERAQLAIKTFVHRIARHIAGHAASLHRLDGIIFTGGIGENSSLI
                     RRLVMEHLAVLGVEIDTEMNNRSNSCGERIVSSENARVICAVIPTNEEKMIALDAIHL
                     GKVNAPAEFA"
     gene            21220..23514
                     /gene="tdcE"
                     /locus_tag="PN977_001067"
     CDS             21220..23514
                     /gene="tdcE"
                     /locus_tag="PN977_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-ketobutyrate formate-lyase/pyruvate
                     formate-lyase"
                     /protein_id="HDI6041124.1"
                     /translation="MKVDIDTSDKLYADAWLGFKGTDWKNEINVRDFIQHNYTPYEGD
                     ESFLAEATPATTELWEKVMEGIRIENATHAPVDFDTNIATTITAHDAGYINQPLEKIV
                     GLQTDAPLKRALHPFGGINMIKSSFHAYGREMDSEFEYLFTDLRKTHNQGVFDVYSPD
                     MLRCRKSGVLTGLPDGYGRGRIIGDYRRVALYGISYLVRERELQFADLQSRLEKGEDL
                     EATIRLREELAEHRRALLQIQEMAAKYGFDISRPAQNAQEAVQWLYFAYLAAVKSQNG
                     GAMSLGRTASFLDIYIERDFKAGVLNEQQAQELIDHFIMKIRMVRFLRTPEFDSLFSG
                     DPIWATEVIGGMGLDGRTLVTKNSFRYLHTLHTMGPAPEPNLTILWSEELPIAFKKYA
                     AQVSIVTSSLQYENDDLMRTDFNSDDYAIACCVSPMVIGKQMQFFGARANLAKTLLYA
                     INGGVDEKLKIQVGPKTAPLMDDVLDYDKVMDSLDHFMDWLAVQYISALNIIHYMHDK
                     YSYEASLMALHDRDVYRTMACGIAGLSVATDSLSAIKYARVKPIRDENGLAVDFEIDG
                     EYPQYGNNDERVDSIACDLVERFMKKIKALPTYRNAVPTQSILTITSNVVYGQKTGNT
                     PDGRRAGTPFAPGANPMHGRDRKGAVASLTSVAKLPFTYAKDGISYTFSIVPAALGKE
                     DPVRKTNLVGLLDGYFHHEADVEGGQHLNVNVMNREMLLDAIEHPEKYPNLTIRVSGY
                     AVRFNALTREQQQDVISRTFTQAL"
     gene            23528..23917
                     /locus_tag="PN977_001068"
     CDS             23528..23917
                     /locus_tag="PN977_001068"
                     /EC_number="3.5.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enamine/imine deaminase"
                     /protein_id="HDI6041125.1"
                     /translation="MKKIIETQRAPGAIGPYVQGVDLGSMVFTSGQIPVCPQTGEIPA
                     DVQDQARLSLENVKAIVVAAGLSVGDIIKMTVFITDLNDFATINEVYKQFFDEHQATY
                     PTRSCVQVARLPKDVKLEIEAIAVRSA"
     gene            23989..25353
                     /gene="tdcG"
                     /locus_tag="PN977_001069"
     CDS             23989..25353
                     /gene="tdcG"
                     /locus_tag="PN977_001069"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HDI6041126.1"
                     /translation="MISAFDIFKIGIGPSSSHTVGPMNAGKSFIDRLESSGLLTATSH
                     IVVDLYGSLSLTGKGHATDVAIIMGLAGNSPQDVVIDEIPAFIELVTRSGRLPVASGA
                     HIVDFPVAKNIIFHPEMLPRHENGMRITAWKGQEALLSKTYYSVGGGFIVEEEHFGLS
                     HDVETSVPYDFHSAGELLKMCDYNGLSISGLMMHNELALRSKAEIDAGFARIWQVMHD
                     GIERGMNTEGVLPGPLNVPRRAVALRRQLVSSDNISNDPMNVIDWINMYALAVSEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFRRPVNERSIARYFLAAGAIGALYKMNASISG
                     AEVGCQGEIGVACSMAAAGLTELLGGSPAQVCNAAEIAMEHNLGLTCDPVAGQVQIPC
                     IERNAINAVKAVNAARMAMRRTSAPRVSLDKVIETMYETGKDMNDKYRETSRGGLAIK
                     VVCG"
     gene            25628..26959
                     /locus_tag="PN977_001070"
     CDS             25628..26959
                     /locus_tag="PN977_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026203.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease"
                     /protein_id="HDI6041127.1"
                     /translation="MEIASNKGVIADASTPAGRAGMSESEWREAIKFDSTDTGWVIMS
                     IGMAIGAGIVFLPVQVGLMGLWVFLLSSVIGYPAMYLFQRLFINTLAESPECKDYPSV
                     ISGYLGKNWGILLGALYFVMLVIWMFVYSTAITNDSASYLHTFGVTEGLLSDSPFYGL
                     VLICILVAISSRGEKLLFKISTGMVLTKLLVVAALGVSMVGMWHLYNVGSLPPLGLLV
                     KNAIITLPFTLTSILFIQTLSPMVISYRSREKSIEVARHKALRAMNIAFGILFVTVFF
                     YAVSFTLAMGHDEAVKAYEQNISALAIAAQFISGDGAAWVKVVSVILNIFAVMTAFFG
                     VYLGFREATQGIVMNILRRKMPAEKINENLVQRGIMIFAILLAWSAIVLNAPVLSFTS
                     ICSPIFGMVGCLIPAWLVYKVPALHKYKGMSLYLIIVTGLLLCVSPFLAFS"
     gene            26987..28297
                     /locus_tag="PN977_001071"
     CDS             26987..28297
                     /locus_tag="PN977_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine dehydratase subunit alpha family protein"
                     /protein_id="HDI6041128.1"
                     /translation="MFDSTLNPLWQRYILAVQEEVKPALGCTEPISLALAAAVAAAEL
                     EGPVERVEAWVSPNLMKNGLGVTVPGTGMVGLPIAAALGALGGNANAGLEVLKDATAQ
                     AIADAKALLAAGKVSVKIQEPCDEILFSRAKVWNGEKWACVTIVGGHTNIVHIETHNG
                     VVFTQQACVTEGEQESPLTVLSRTTLAEILKFVNEVPFAAIRFILDSAKLNCALSQEG
                     LSGNWGLHIGATLEKQCARGLLAKDLSSSIVIRTSAASDARMGGATLPAMSNSGSGNQ
                     GITATMPVVVVAEHFGADDERLARALMLSHLSAIYIHNQLPRLSALCAATTAAMGAAA
                     GMAWLVDGRYETISMAISSMIGDVSGMICDGASNSCAMKVSTSASAAWKAVLMALDDT
                     AVTGNEGIVAHDVEQSIANLCALASHSMQQTDRQIIEIMASKAR"
     gene            complement(28430..28594)
                     /locus_tag="PN977_001072"
     CDS             complement(28430..28594)
                     /locus_tag="PN977_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6041129.1"
                     /translation="MSKKSAKKRQPVKPVVAKEPSRTAKNFGYEEMLSELEAIVADAE
                     TRLAEDEATA"
     gene            complement(28617..29318)
                     /locus_tag="PN977_001073"
     CDS             complement(28617..29318)
                     /locus_tag="PN977_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HDI6041130.1"
                     /translation="MITTRTARQCGQADYGWLQARYTFSFGHYFDPKLLGYASLRVLN
                     QEVLAPGAAFQPRTYPKVDILNVILDGEAEYRDSEGNHVQASAGEVLLLSTQPGVSYS
                     EHNLSKDKSLTRMQLWLDACPQRENPLIQKLALNMSKQQLIASPEGGMGSLQLRQQVW
                     LHHIVLDKGESANFQLHGPRAYLQSIHGKFHALTHHEEKAALTCGDGAFIRDEANITL
                     VADSPLRALLIDLPV"
     gene            29423..30319
                     /gene="yhaJ"
                     /locus_tag="PN977_001074"
     CDS             29423..30319
                     /gene="yhaJ"
                     /locus_tag="PN977_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YhaJ"
                     /protein_id="HDI6041131.1"
                     /translation="MAKERALTLEALRVMDAIDRRGSFAAAADELGRVPSALSYTMQK
                     LEEELDVVLFDRSGHRTKFTNVGRMLLERGRVLLEAADKLTTDAEALARGWETHLTIV
                     TEALVPTPAFFPLIDKLAAKANTQLAIITEVLAGAWERLEQGRADIVIAPDMHFRSSS
                     EINSRKLYTLMNVYVAAPDHPIHQEPEPLSEVTRVKYRGIAVADTARERPVLTVQLLD
                     KQPRLTVSTIEDKRQALLAGLGVATMPYPMVEKDIAEGRLRVVSPESTSEIDIIMAWR
                     RDSMGEAKSWCLREIPKLFSGK"
     gene            complement(30370..30726)
                     /locus_tag="PN977_001075"
     CDS             complement(30370..30726)
                     /locus_tag="PN977_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HDI6041132.1"
                     /translation="MQWYLAVLKNYVGFSGRARRKEYWMFTLINAIVGAIINVIQLIL
                     GLEFPFLSLIYLAATIIPVIALCVRRLHDTDRSGAWALLYLVPVIGWLVLFVFACLEG
                     NSGSNRYGNDPKFGSN"
     gene            complement(30968..31333)
                     /locus_tag="PN977_001076"
     CDS             complement(30968..31333)
                     /locus_tag="PN977_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HDI6041133.1"
                     /translation="MDWYLKVLKNYVGFRGRARRKEYWMFILVNIIFTFVLGLLDKML
                     GWQRAGGEGILTTIYGILVFLPWWAVQFRRLHDTDRSAWWALLFLIPFIGWLIIIVFN
                     CQAGTPGENRFGPDPKLEP"
     gene            complement(31616..32602)
                     /gene="yqjG"
                     /locus_tag="PN977_001077"
     CDS             complement(31616..32602)
                     /gene="yqjG"
                     /locus_tag="PN977_001077"
                     /EC_number="1.8.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionyl-hydroquinone reductase YqjG"
                     /protein_id="HDI6041134.1"
                     /translation="MGQLIDGVWHDTWYDTKSTGGKFQRSASAFRNWLTADGAPGPTG
                     KGGFIAEKDRYHLYVSLACPWAHRTLIMRKLKGLEPFISVSVVNPLMLENGWTFDDSF
                     PGATGDTLYQHEFLYQLYLHADPHYSGRVTVPVLWDKKNHTIVSNESAEIIRMFNTAF
                     DALGAKAGDYYPPALQTKIDELNGWIYDTVNNGVYKAGFATSQQAYDEAVAKVFESLA
                     RLEQILGQHRYLTGNQLTEADIRLWTTLVRFDPVYVTHFKCDKHRISDYLNLYGFLRD
                     IYQMPGIAETVNFDHIRNHYFRSHKTINPTGIISIGPWQDLDEPHGRDVRFG"
     gene            complement(32850..33242)
                     /locus_tag="PN977_001078"
     CDS             complement(32850..33242)
                     /locus_tag="PN977_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008503138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HDI6041135.1"
                     /translation="MKKLEDVGVLVARILMPILFITAGWGKITGYAGTQQYMEAMGVP
                     GFMLPLVILLEFGGGLAILFGFLTRTTALFTAGFTLLTAFLFHSNFAEGVNSLMFMKN
                     LTISGGFLLLAITGPGAYSIDRLLNKKW"
     gene            complement(33428..33727)
                     /locus_tag="PN977_001079"
     CDS             complement(33428..33727)
                     /locus_tag="PN977_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqjK-like family protein"
                     /protein_id="HDI6041136.1"
                     /translation="MSSKVERERRKAQLLSQIQQQRLDLSASRREWLEATGAYDRRWN
                     MLLSLRSWALVGSSVMAIWTIRHPNMLVRWARRGFGVWSAWRLVKTTLKQQQLRG"
     gene            complement(33717..34121)
                     /locus_tag="PN977_001080"
     CDS             complement(33717..34121)
                     /locus_tag="PN977_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HDI6041137.1"
                     /translation="MADTHHAQGPGKSVLGIGQRIVSIMVEMVETRLRLAVVELEEEK
                     ANLFQLLLMLGLTMLFAAFGLMSLMVLIIWAVDPQYRLNAMIATTVVLLLLALIGGIW
                     TLRKSRKSTLLRHTRHELANDRQLLEEESREQ"
     gene            complement(34124..34429)
                     /locus_tag="PN977_001081"
     CDS             complement(34124..34429)
                     /locus_tag="PN977_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 domain-containing protein"
                     /protein_id="HDI6041138.1"
                     /translation="MSKEHTTEHLRAELKSLSDTLEEVLSSSGEKSKEELSKIRSKAE
                     QALKQSRYRLGETGDAIAKQTRVAAARADEYVRENPWTGVGIGAAIGVVLGVLLSRR"
     gene            complement(34467..34835)
                     /locus_tag="PN977_001082"
     CDS             complement(34467..34835)
                     /locus_tag="PN977_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1090 domain-containing protein"
                     /protein_id="HDI6041139.1"
                     /translation="MKYRIALAVSLFALSAGSYATTLCQEKEQNILKEISYAEKHQNQ
                     NRIDGLNKALSEVRANCSDSQLRADHQKKIAKQKDEVAERQQDLAEAKQKGDADKIAK
                     RERKLAEAQEELKKLEARDY"
     gene            complement(34982..35365)
                     /gene="mzrA"
                     /locus_tag="PN977_001083"
     CDS             complement(34982..35365)
                     /gene="mzrA"
                     /locus_tag="PN977_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EnvZ/OmpR regulon moderator MzrA"
                     /protein_id="HDI6041140.1"
                     /translation="MQIPRMSLRQLAWSGAVLLLVGTLLLAWSAVRQQESTLAIRAVH
                     QGTTMPDGFSIWHHLDAHGIPFKSITPKNDTLLITFDSSDQSAAAKAVLDRTLPHGYI
                     IAQQDNNSQAMQWLTRLRDNSHRFG"
     gene            complement(35369..36031)
                     /locus_tag="PN977_001084"
     CDS             complement(35369..36031)
                     /locus_tag="PN977_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HDI6041141.1"
                     /translation="MELLTQLLQALWAQDFETLANPSMIGMLYFVLFVILFLENGLLP
                     AAFLPGDSLLVLVGVLIAKGAMGYPQTILLLTVAASLGCWVSYIQGRWLGNTRTVQNW
                     LSHLPAHYHQRAHHLFHKHGLSALLIGRFIAFVRTLLPTIAGLSGLNNARFQFFNWMS
                     GLLWVLILTTLGYMLGKTPVFLKYEDQLMSCLMLLPVVLLVFGLAGSLVVLWKKKYGN
                     RG"
     gene            complement(36376..37152)
                     /gene="exuR"
                     /locus_tag="PN977_001085"
     CDS             complement(36376..37152)
                     /gene="exuR"
                     /locus_tag="PN977_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ExuR"
                     /protein_id="HDI6041142.1"
                     /translation="MEITEPRRLYQQLAADLKERIEQGVYLVGDKLPAERFIADEKNV
                     SRTVVREAIIMLEVEGYVEVRKGSGIHVVSNQPRHQQSADNNMEFANYGPFELLQARQ
                     LIESNIAEFAATQVTKQDIMKLMAIQEQARGEQCFRDSEWDLQFHIQVALATQNSALA
                     AIVEKMWTQRSHNPYWKKLHEHIDARTVDNWCDDHDQILKALIRKDPHAAKLAMWQHL
                     ENTKIMLFNETSDDFEFNADRYLFAENPVVHLDTATSGSK"
     gene            37442..38158
                     /locus_tag="PN977_001086"
     CDS             37442..38158
                     /locus_tag="PN977_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000225871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HDI6041143.1"
                     /translation="MVAKINRLLRLGPVLLLCTSFFAQSNMTVYPMSVSINSQGEGSV
                     RVISKTNEVQYIKATVFRIDNPSTPQEKEIEVTSGDANQLVIMPPKFALPGGSSKTVR
                     FVAMEPEQKEKNYRVKFEAVPSIDDATDNKKDLSMQLTVNLIWGIVVSVPPQQPVAKL
                     DINADKKLINNGNQRLKILTIAYCKNSGSAECRTQTVNKNIFPGQERVLESISGYDKI
                     VVKYNNWITKDNGEFELAVH"
     gene            38188..38688
                     /locus_tag="PN977_001087"
     CDS             38188..38688
                     /locus_tag="PN977_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000755097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HDI6041144.1"
                     /translation="MKKVFAKSLLVAAMFSVAGSALAVQKDITVTANVDAALDMTQTD
                     NTALPKAVEMQYLPGQGLQSYQLMTKIWSNDTTKDVKMQLVSPAQLVQSLDASKIVPL
                     TVTWGGEEIKADAATTFTATKIFASDALTNGSLAKPLMFSQATKGVLETGIYRGVVSI
                     YLSQAL"
     gene            38779..41463
                     /locus_tag="PN977_001088"
     CDS             38779..41463
                     /locus_tag="PN977_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TcfC E-set like domain-containing protein"
                     /protein_id="HDI6041145.1"
                     /translation="MDKKLLALLILASLSPAKATLTKIPAGFEVIAQGQQEYIEVYFA
                     GKSLGKYYAMVNLDTVTFLDSSSLYSKLELSTDDQKIAHTVKEKLSQPLARHGELACG
                     FVRTDSGCGFLNTETVEIIYNDEESSATLFLNPQWSSAFNSKSLYLNPDKNTVNAFIH
                     QQDINVLVQDDYQSLSIQGNGALGITENSYIGAHWNFDGYDADDVSDNNVDVSDLYYR
                     YDFLRRYYVQAGRMDNRTLFNAQGGNFTFNFLPLGAIDGMRIGSTLSYLNQTQSQQGT
                     PVMILLSRNSRVDAYRNEQLLGSFYLNSGSQFIDTSSFPPGSYSVALKVYENNQLTRT
                     ELVPFTKTGGLTDGNAQWFLQAGKTTSQVSDDESSAYQLGVRLPLHPQYELYAGLANA
                     DDVSAFELGNNWTADLGGAGNLAISASVFRNDDGGKGDMQQANWSHPGWPTLGFYRTN
                     SDGDACTTDNRESYNALSCYESISATVSQNFVGWNMMLGYTRTQNNTDDSLRWDKQQS
                     FENNYLRQTSAQSISETVQLSASRAFVMRDWILSTSLGVFHRNDNGGGSDDNGLYLSF
                     SLSDTPTMDSNNNSHSTNVSTDYRYSDQDGDQTSWQLSHTFYNDSFSHKELGVTVGGL
                     NTDTINSAVNGRWDGQYGNVYATVSDSYDRQNHDHLSAFTGTYSSTLAVSRYGINLGA
                     SGSDDLLGAVLVDVKGFSEQDEQSQGLQLEARVAGSRTLQLGQSDSVLFPYPGFQSGF
                     VEVNDSNQGNQQGTTNIINGAGNRELMLLPGKLRYREVSASFNYNYIGRLLLPASVEK
                     FPLVGLNSAMLLVAEDGGFTLEISSGEKELYLLSGQQFLKCPLNVLKKRASIRYSGDV
                     NCSVVSYSQLPESIQVQAQLKQPKLRGNVQTAQREVAP"
     gene            41460..42554
                     /locus_tag="PN977_001089"
     CDS             41460..42554
                     /locus_tag="PN977_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001352530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein CblD"
                     /protein_id="HDI6041146.1"
                     /translation="MRNRLIAVILCLFGMVAGGHATPNVTAEITYDLAAGRADYYFWN
                     REDPPAVSYNTTWSSYKCDFPDVQQTCTASGNLSTVKIYLTEKRSGMRWPVKLKGYVD
                     AEVWNPGGVCNGWSTQIALANGTGYQCKSASDGYIQHLASAKPMTLYLEQSEMKNLPI
                     GGVWEGSVKLQFTNPSTDYRADITLNVLDPNHIDVFFPEFAHATPRVQLDLHPTGSVN
                     GNNYAQDLTMLDMCLYDGFNGNGLSYEILLKDEGRTAAGRSNGEFSIYRQGASSTDEG
                     ERIDYRVKMYDPESGGQIDVRNNESMVWTNINLKRVRPVVLPGIRYAVMCVPTPLTLV
                     VDKFNVTAKQAGYYMGKLSVIFTPSLPTIN"
     gene            complement(42630..43928)
                     /gene="exuT"
                     /locus_tag="PN977_001090"
     CDS             complement(42630..43928)
                     /gene="exuT"
                     /locus_tag="PN977_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708898.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexuronate transporter ExuT"
                     /protein_id="HDI6041147.1"
                     /translation="MRKIKGLRWYMIALVTLGTVLGYLTRNTVAAAAPTLMEELNIST
                     QQYSYIIAAYSAAYTVMQPVAGYVLDVLGTKIGYAMFAVLWAVFCGATALAGSWGGLA
                     VARGAVGAAEAAMIPAGLKASSEWFPAKERSIAVGYFNVGSSIGAMIAPPLVVWAIVM
                     HSWQMAFIISGALSFIWAMAWLIFYKHPRDQKHLTDEERDYIINGQEAQHQVDTAKKM
                     SVGQILRNRQFWGIALPRFLAEPAWGTFNAWIPLFMFKVYGFNLKEIAMFAWMPMLFA
                     DLGCILGGYLPPLFQRWFGVNLIVSRKMVVTLGAVLMIGPGMIGLFTNPYVAIMLLCI
                     GGFAHQALSGALITLSSDVFGRNEVATANGLTGMSAWLASTLFALVVGALADTIGFSP
                     LFAVLAVFDLLGALVIWTVLQNKPAIEVAQESHNDPAPQH"
     gene            44411..45823
                     /gene="uxaC"
                     /locus_tag="PN977_001091"
     CDS             44411..45823
                     /gene="uxaC"
                     /locus_tag="PN977_001091"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HDI6041148.1"
                     /translation="MTPFMTEDFLLDTEFARRLYHDYAKDQPIFDYHCHLPPQQIAED
                     YRFKNLYDIWLKGDHYKWRAMRTNGVAERLCTGDASDREKFDAWAATVPHTIGNPLYH
                     WTHLELRRPFGITGKLLSPSTADEIWNECNELLAQDNFSARGIMQQMNVKMVGTTDDP
                     IDSLEHHAEIAKDGSFTIKVLPSWRPDKAFNIEQATFNDYMAKLGEVSDTDIRRFADL
                     QTALTKRLDHFAAHGCKVSDHALDVVMFAEANEAELDSILARRLAGETLSEHEVAQFK
                     TAVLVFLGAEYARRGWVQQYHIGALRNNNLRQFKLLGPDVGFDSINDRPMAEELSKLL
                     SKQNEENLLPKTILYCLNPRDNEVLGTMIGNFQGEGMPGKMQFGSGWWFNDQKDGMER
                     QMTQLAQLGLLSRFVGMLTDSRSFLSYTRHEYFRRILCQMIGRWVEAGEAPADINLLG
                     EMVKNICFNNARDYFAIELN"
     gene            45838..47325
                     /gene="uxaA"
                     /locus_tag="PN977_001092"
     CDS             45838..47325
                     /gene="uxaA"
                     /locus_tag="PN977_001092"
                     /EC_number="4.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HDI6041149.1"
                     /translation="MQYIKIHALDNVAVALADLAEGTEVSVDNQTVTLRQDVARGHKF
                     ALTDIAKGANVIKYGLPIGYALADIAAGEHVHAHNTRTNLSDLDQYRYQPDFQDLPAQ
                     AADREVQIYRRANGDVGVRNELWILPTVGCVNGIARQIQNRFLKETNNAEGTDGVFLF
                     SHTYGCSQLGDDHINTRTMLQNMVRHPNAGAVLVIGLGCENNQVAAFRETLGDIDPER
                     VHFMICQQQDDEIEAGIAHLHQLYNVMRNDKREPGKLSELKFGLECGGSDGLSGITAN
                     PMLGRFSDYVIANGGTTVLTEVPEMFGAEQLLMDHCRDEATFEKLVTMVNDFKQYFIA
                     HDQPIYENPSPGNKAGGITTLEDKSLGCTQKAGSSVVVDVLRYGERLKTPGLNLLSAP
                     GNDAVATSALAGAGCHMVLFSTGRGTPYGGFVPTVKIATNSELAAKKKHWIDFDAGQL
                     IHGKAMPQLLEEFIDTIVEFANGKQTCNERNDFRELAIFKSGVTL"
     gene            47408..47959
                     /locus_tag="PN977_001093"
     CDS             47408..47959
                     /locus_tag="PN977_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgjV family protein"
                     /protein_id="HDI6041150.1"
                     /translation="MTAYWLAQGVGVIAFLIGITTFFNRDERRFKKQLSVYSAVIGVH
                     FFLLGTYPAGASAILNAIRTLITLRTRSLWVMAIFIVLTGGIGLAKFHHPVELLPVIG
                     TIVSTWALFRCKGLTMRCVMWFSTCCWVIHNFWAGSIGGTMIEGSFLLMNGLNIIRFW
                     RMQKRGIDPFKVEKTPSAVDERG"
     gene            complement(47963..49207)
                     /gene="sstT"
                     /locus_tag="PN977_001094"
     CDS             complement(47963..49207)
                     /gene="sstT"
                     /locus_tag="PN977_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HDI6041151.1"
                     /translation="MTTQRSPGLFRRLAHGSLVKQILVGLVLGILLAWISKPAAEAVG
                     LLGTLFVGALKAVAPILVLMLVMASIANHQHGQKTNIRPILFLYLLGTFSAALAAVIF
                     SFAFPSTLHLSSSAGDISPPSGIVEVMRGLVMSMVSNPIDALLKGNYIGILVWAIGLG
                     FALRHGNETTKNLVNDMSNAVTFMVKLVIRFAPIGIFGLVSSTLATTGFSTLWGYAQL
                     LVVLVGCMLLVALVVNPLLVWWKIRRNPFPLVLLCLRESGVYAFFTRSSAANIPVNMA
                     LCEKLNLDRDTYSVSIPLGATINMAGAAITITVLTLAAVNTLGIPVDLPTALLLSVVA
                     SLCACGASGVAGGSLLLIPLACNMFGISNDIAMQVVAVGFIIGVLQDSCETALNSSTD
                     VLFTAAACQAEDDRLANSALRN"
     gene            complement(49531..50496)
                     /gene="alx"
                     /locus_tag="PN977_001095"
     CDS             complement(49531..50496)
                     /gene="alx"
                     /locus_tag="PN977_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family membrane protein Alx"
                     /protein_id="HDI6041152.1"
                     /translation="MNTVGTPLLWGGFAVVVTIMLAIDLLLQGRRGAHAMTMKQAAAW
                     SLVWVTLSLLFNAAFWWYLVQTEGRAVADPQALAFLTGYLIEKSLAVDNVFVWLMLFS
                     YFSVPAALQRRVLVYGVLGAIVLRTIMIFTGSWLISQFDWILYIFGAFLLFTGVKMAL
                     AHEDESGIGDKPLVRWLRGHLRMTDTIDNEHFFVRKNGLLYATPLMLVLILVELSDVI
                     FAVDSIPAIFAVTTDPFIVLTSNLFAILGLRAMYFLLAGVAERFSMLKYGLAVILVFI
                     GIKMLIVDFYHIPIAVSLGVVFGILVMTFIINAWVNYRHDKQRVG"
     gene            complement(50780..51766)
                     /locus_tag="PN977_001096"
     CDS             complement(50780..51766)
                     /locus_tag="PN977_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417558.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HDI6041153.1"
                     /translation="MIRFAVIGTNWITRQFVEAAHESGKYKLTAVYSRSLEQAQHFAN
                     DFSVEHLFTSLEAMAESDAIDAVYIASPNSLHFSQTQLFLSHKIHVICEKPLASNLAE
                     VDAAIACARENQVVLFEAFKTASLPNFHLLRQALPKVGKLRKVFFNYCQYSSRYQRYL
                     DGENPNTFNPAFSNGSIMDIGFYCLASAVALFGEPKSVQATASLLASGVDAHGVVVMD
                     YGDFSVTLQHSKVSDSVLASEIQGEAGSLVIEKLSECQKVCFVPRGSQMQDLTQPQHI
                     NTMLYEAELFATLVDEHLVDHPGLAVSRITAKLLTEIRRQTRVIFPADSVKL"
     gene            complement(51845..52528)
                     /locus_tag="PN977_001097"
     CDS             complement(51845..52528)
                     /locus_tag="PN977_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001183047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin high temperature exclusion protein"
                     /protein_id="HDI6041154.1"
                     /translation="MQPRLLLRICFSRRTLKIGCLLLLIAGATIFIADRVMVNASQQL
                     TWSDVNAVPARNVGLLLGARPGNRYFTRRIDSAAELYHAGKVKWLLVSGDNGRKNYDE
                     ASGMQQALIAKGVPAKVIFCDYAGFSTLDSVVRANKVFSENHITIISQEFHNQRAIWL
                     AKQYGIDAIGFNAPDLNMKHGLYTQLREKLARVSAVIDAKILHRQPKYLGPLVMIGPF
                     SEHGCPAKE"
     gene            complement(52605..53108)
                     /locus_tag="PN977_001098"
     CDS             complement(52605..53108)
                     /locus_tag="PN977_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HDI6041155.1"
                     /translation="MSNLTYLQGYPEQLLSQVRTLINEQRLGDVLAKRYPGTHDYATD
                     KALWQYTQDLKNQFLRNAPPINKVMYDNKIHVLKNALGLHTAVSRVQGGKLKAKAEIR
                     VATVFRNAPEPFLRMIVVHELAHLKEKEHNKAFYQLCCHMEPQYHQLEFDTRLWLTQL
                     SLGQDKI"
     gene            53193..>54210
                     /gene="rlmG"
                     /locus_tag="PN977_001099"
     CDS             53193..>54210
                     /gene="rlmG"
                     /locus_tag="PN977_001099"
                     /EC_number="2.1.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(1835)-N(2))-methyltransferase
                     RlmG"
                     /protein_id="HDI6041156.1"
                     /translation="MSHLDNGFRSLTLQRFPATDDVNPLQAWEAADEYLLQQLDDTEI
                     RGPVLILNDAFGALSCALAEHKPYSIGDSYISELATRENLRLNGIDESSVKFLDSTAD
                     YPQQPGVVLIKVPKTLALLEQQLRALRKVVTPDTRIIAGAKARDIHTSTLELFEKVLG
                     PTTTTLAWKKARLINCTFNEPPLVDAPQTVSWKLEGTDWTIHNHANVFSRTGLDIGAR
                     FFMQHLPENLEGEIVDLGCGNGVIGLTLLDKNPQAKVVFVDESPMAVASSRLNVETNM
                     PEALDRCEFMINNALSGVEPFRFNAVLCNPPFHQQHALTDNVAWEMFHHARRCLKING
                     ELYIV"
BASE COUNT        13648 a        13156 c        13819 g        13587 t
ORIGIN      
        1 gacgcgtcag cgtcgcatcc gacaccgctg cacagatcaa aaagaaagaa aaagaaaggc
       61 cttacggggg aagtgaaggc ccaataaagg agcaaaccat caaaacgaag ctaattgacc
      121 ctgaatccag atttgttgca catgcagccc gctatccagc gcaacgacgc tggcgcgttt
      181 gcccggttta agcgatccca gaacaccatc aacacccagc atacgcgccg gatgcagcga
      241 tgccatatgg atggcttccg caggcgttac gcccgtcaac tcgaccatgt tgcgcactgc
      301 cgcatcgaca gacagcgtac tgcccgccag cccaccggac gcggtacgga caacgccatc
      361 gtgcatctgc acttcttcac cacataacgt atagcgacca tccggcatcc cggctgcctg
      421 catcgcgtcg gtgatcagca cgattctctc tttcgcacag caacaacaca gcgacatcgc
      481 cgccggatgt acatgatgac catcggctat cagttccagc caggcgcgct tgtccgttaa
      541 tcccgcgcca accatccccg gttcgcggtg atgtaaacct gtcatcccgt tatagcaatg
      601 caccaggccg tcggcaccag cgtcaaacgc ggcacgggtt tgttgccagg tcgccgcgct
      661 atgccccagc atcacccgta cgttttgctg tttaagatag cggatggcct gcaatgcccc
      721 ttctttttct ggtgccagcg ctactacgcg caaggtgtgc tgagaaacgg caatcaattg
      781 atccagctcg gcaatttcca gctcgcgaaa cagctccggc ggatgcgcgc ctttattctg
      841 cggcgtgaag tacggtcctt cgagataact ccccagcact tgcgcaccag gtccgccacg
      901 ctggcagcgt agagcaatac gtttcagcgc cgcatgaatg gtattaagcg gcgcggttac
      961 ggtggtcggt aaccaactgc cgacaccttc gcgtgccttg tgcattgcca gcttgtcgag
     1021 cgcatccggc gcatcatcca ttacatcaac gcccgcacca ccgtgtacat gggtgtcgat
     1081 gtaagcggga cagagcagtt ccgcatcgcg ttcagtcacg cccactggaa tcggttcgat
     1141 tgctgcgatt acaccgtcag caatacgcaa ctgatggtca tcgagccatc cctcttcagt
     1201 cagcagcctt ctggcgcgca gaacgtgtgt catattcctt cctcgaccgg acattcctca
     1261 tggcagcgac caagttcgtc caccagactg gttagcccac ggtgtccaca ctccagcgcc
     1321 tgcacacgga actcttcacc gcttaaccct tcgcgttcca gcaccatttc cagtaatagt
     1381 tgcaaatttg tgccggtgat cacttcacag cccggttttt gcatcgccag cgttgaagct
     1441 acgcgaaacg gcgtgccgcc cagcaaatcc gtcagaaaaa caatgccgtc ttcacaatcc
     1501 agttggacga tggcctcttc aagctgcgat gtaagcagcg cggtgcttga ggtttccgga
     1561 aaatcgatgg cgataaactg cgattgctca ccgagaattt gcttcattgc tttttccatg
     1621 ccgctggcaa atccgccatg ccctgtcaaa ataatactta acatctaata cctcattgta
     1681 tttaaaggaa atggcagaat ttaccgacga tgccgagaac cacggtaacc gcaatcaggc
     1741 gcagcggact ccagccgcgg cgtaccagcc agaacatggt cagggtgtag accagcggta
     1801 agaaggctgg catcagtttg tcgataacat cagcctgtaa cttaaccact gcgtcgcccg
     1861 cggtgatttc cagcgtggtg cttaaacgaa cataggttgc caccagcgcg ccgattaccg
     1921 tcatcccaac gatagatgcc gcatggccga cttttttggt atttgcttta atgagtggaa
     1981 tcgccgccac gcccatgcgg taagcataat gcgccagacc aaaacgcagc cccaggtgca
     2041 ccacgttgaa cagcacaata aagacgacag cgccaagaat agagccttgt agcgcgaggc
     2101 ttgcacctat cccgccacaa atcggcagta gcgttagcca gaacatcgca tcgccaatcc
     2161 cgccgagcgg cgcaccgacg gcaattttgg tgctctgaat actgttaacg tcctgcttag
     2221 aacgctccat cgcaaggata atgccgataa caaacgtcac cagaaacgga tgggtattga
     2281 agaaacccat atgccccttc atggcgcgcg ccaggtcgcg tttattagtg tggatctttt
     2341 tcagagcagg cagcagaccg tacaaccagc cagaagcctg catacgttcg tagttaaaag
     2401 acgcttgtaa cagcatggaa cgccaggcga cgcggttgat atcttttttc gtcagctctg
     2461 cgccaatgct ctgatcttca tacacgtttt cattgacacc tgtcagcagt gtttcttcgt
     2521 tttcactgac gttcggcagg gtagtttgat tagatgccat cttcaaattc ctctttctgt
     2581 gccgcaggtt gagtcggttc aggagattta cgcagcaagt cgatcagcgc catcgccagc
     2641 gcggcggcag caatagccag caccggtaac ttgagccagg ctgcggcaac aaagcccagg
     2701 atgaagtagg ggatgtagac gtttttcatc atgattttca gcagcacggc aaaaccgatt
     2761 gctggcatga tgccacccgc gacgccgagg ccgtcgatta atcgttgcgg caggacatca
     2821 atgatggttt tggcatgttc tgcgccgaag tagataggca ggaaagcgca gagaaaatag
     2881 aacgtgccga gtgccagcaa tgccagatag ttcacgcgtt caatgccgcg agtatcggcg
     2941 ttctccgcca tccggtcgca gcgggacatc acgccggaca tcaccgagaa caggaaggta
     3001 atccccatct gtactgcgac agcgaaaggt acggcgacac cgaccgcgac atccggtttc
     3061 acgccagtag taatggcaaa cgccgtgccg acgatagtac cgataatcac gttaggcggt
     3121 tgtgcgcctg ccagcggggc cagccccatc cacaccagtt ccagcgtacc gccggttaaa
     3181 ataccggtat gcagatcgcc aagtaccagt ccgaccaacg ggccgaggac caccgggcgg
     3241 tgcatatggg ttaggccgtt aaacatatcc aggccagcga taaaggcgat aatgcccaac
     3301 gcgaatgcct gcaacagact gatttccatg atgaatccct caaagtaatt taaagaggtc
     3361 cactgcaggc tctgttggga cgccctgaac gaagcattcc accccagcgg ctttcaggtc
     3421 gttaaatgct gcgatatcac ccgcatccac agaaaccgtt ttggcgattt gttgcttgcc
     3481 attggcgtag tgcatattgc caacgttaat gcgattcacc ggaacgccac ctttcaccag
     3541 cgtcaggaaa tcggcaggtg ttttacaaac cagcaggatt ttctgtcgat cggcggcgcg
     3601 atgaatgttg tcgatgactt tttgcagcgt ccagaaacgt acggcgatcc cttctgccag
     3661 taccatttcc atcaggtttt gttgtaccgg atcttcggca acctcatcgt tggctaccag
     3721 caccagattt gcccccgcaa atccgaccca ctgaacgccg acctgaccgt gaatcaaacg
     3781 ctcatcaatc cggcttaaaa caatatttgg catagcgtgt tcctctttgt tttgcttatt
     3841 ggccttcaca ggctgtgtgg tactgcgcca ggatgtcctg aatatggttg ataatgagtt
     3901 cccgtggtgt tggctgcagc tcgccggagc gaactttcac gtattgcagc ggcaaatact
     3961 ggctgatcag cggtagcgga attggtgaat ccgccagatt acgaaccaga tgagcgaaag
     4021 cgtcatcaat ctggctgtcc ggccagtaat agcgcacgcg atccgagtag ctataaccac
     4081 gcgccagacg acgcgcgttg ccgtcaccgt ggtagtggct ttgccagtat tccgggcggt
     4141 cgagcatcac gttttccagc acctgacgca gaccagaaca ggcttttgct ggtaccagtt
     4201 cttcttcaat cgccgccaga gagaacagag cttcacgcag agcaaaggtc agcgccgggc
     4261 caactttcag aatggcaaag tggtcaatca ccagctggcg cagcgattgc ggcgtttggt
     4321 aatcagtaga gtgcgcttcg aaaatcagcg tttcgtagtt ttcgaccatc tggcttaagg
     4381 cggtcgcttt gacgggctga taatcaataa cgttggtgtg atcgaattcg acgccgggtt
     4441 gaaccaccag ggcaataatg cgtggccaga tggcactcag accttgcttt tcaaaggcgt
     4501 gacgatgggc ttccagcgtg gcgcgggcgg catccggcgt ggtgaccgcc agctcgctta
     4561 aggtttcatg cgcgccgcca ggtaccggta cttcggtacc aatgacatac tccagatcgg
     4621 ccgcgccaaa atgttcacgg caggtttctt ccgccacttt cgccagacga gcggcgcgtt
     4681 cggccacgat gtcatcagtt aagggaatcg gatcgtcctg acaggacatg ctgcaatcaa
     4741 ggtgaatttt tttgaatcct gccgccacgt agcttttaat caaatcatcg gcattggcca
     4801 ttgcctgagc ggcaggcagg ttttgccagc ggtttggccc cagatggtcg ccacccagaa
     4861 tcaacgcatc ctgtgggaaa ttcaacgagt cggcgagctg acaaacaaag ccgcgaaaat
     4921 cggcgggcgt cattccggta taaccgccga actggtccac ctgattggag gttgcttcaa
     4981 tcagtaacgg cgtttggttt gcactggcgt agcggattgc agcttccagc accagcggat
     5041 gtgcggaaca aacggcataa atcccatttg ttttgccctc tttgtgctgt ctcaccattt
     5101 ctgtcagatg tttcactttc ctctccagtt cagattactg cgatattaat ctttcgtttt
     5161 gtttcagttg atactgcgcc atggtgagat aaaaataaaa cgaaagataa tggttttctg
     5221 atctgattca caaaagaaac ataatgaaag ccactgaaac gagagtagct tgcgccaatg
     5281 ggtaaggtgg gcttgcattt gcttaataga aaggcgttaa taggcaaaac gaaatgaaac
     5341 gaaagtttta cgaaaggact tactatgagt aataccgacg cttcaggtga gaagcgaatg
     5401 acaggcacca gcgagcgacg agaacagatc attcagcgtc tgcgacagca agggagtgtg
     5461 caggttaacg atctgtcggc attgtatggc gtatctactg tgacgatccg caacgatctg
     5521 gcgtttctgg aaaagcaggg gatcgctgtg cgtgcctatg gtggcgcgtt gatctgcgat
     5581 agcacaacgc cgtcagtcga gccatcagtg gaagataaaa gcgcactgaa caccgcgatg
     5641 aaacgcagcg ttgcgaaagc tgccgttgag ttgattcagc ctggtcatcg ggtgatcctc
     5701 gactccggga ccaccacttt tgagattgct cgtctgatgc gcaagcacac cgacgtaatt
     5761 gcgatgacca acggtatgaa cgtggctaat gcgttgctgg aagcggaagg cgttgagctg
     5821 ctgatgaccg gggggcattt gcgccgtcag tcgcaatctt tttacggtga tcaggctgag
     5881 caatcgctgc aaaattacca cttcgatatg ctgtttcttg gtgtagatgc gatcgatctg
     5941 gagcgcggcg tcagcacgca taatgaagat gaagcccgtt taaaccgtcg gatgtgcgaa
     6001 gttgcggaac ggatcatcgt ggtcaccgat tccagtaagt tcaaccgttc cagtttacat
     6061 aagatcattg atactcaacg tatcgacatg atcattgttg atgaaggcat tcctgcggat
     6121 agtctggaag gactgcgaaa ggctggggtt gaagtgattc tggtcgggga gtgagaaagg
     6181 gtgcccgctg gtcaacacga atgcgttcgg cgggcattgt gaacaccatc aatgggtttc
     6241 ttctgtctct cgggtgaggg tttcccagtc ggcaggagga tgtcctcttt ttaacatttt
     6301 gctgaatacg gtataggcat ctgttttttt accgtaggtg cgcagcgtgt tttcatcgtt
     6361 catccatccc agaatgatga ctttctcttt ttcactataa cgaaagaaga gacgataacg
     6421 accagcacca aattttaccc gtgaccagtc tttatttttc ccagagccta acgacttgcc
     6481 atgacggaat gccggtgatg atggattgac cgtgatatgc tcctcaatca ctttatggac
     6541 taccgccaat aactttgtgg cggcttttct ctgataattt tcaggatctt ttccctttaa
     6601 tgtctcgact tcggcaacta aagcgtcgta ggtttcctga aaacagggat gagcatatag
     6661 cgcccaacca ttaacccttt gtggaaaatc cattattcgt cgtcgccaat ctcatcgtca
     6721 atgttgacgt ccatgccagc gacaagtttc tttccttgtt gagtgtcgaa tggacgagtt
     6781 ttttgcgggt tgttctggat atctgcatcc agaaaacgca aaaatgcatt catagtatga
     6841 tcctcctgtt catctcccag tcggcacata aatacttgcc caccaggcag aatttcgtaa
     6901 tgaatgctgt ccaggcctgg cttcagtttt aaggcctcac gcactggcgc ggggatagtt
     6961 gtttgtccgc gtatcgtgac ctttgattca gtggtcagta cagcgttaga gcgagcatta
     7021 gcgggcatta tttactgtcc ttttacagcc tgttttctgc tcaaattata agcttgaact
     7081 aaggtaatgc aaatgcatta ttaatggatg tgtggacttt aaacaataaa aacatggcac
     7141 taaggccatg ttttgcgagg gacgttccaa aagaaatcag gtcaccggtg ctgggttaaa
     7201 caccgccagc tggttatgca gtccccattg atccgagaaa gttttcttct tcccgctggc
     7261 gacgtcgaga ataaagtgga acaacttcca gcccacttct tcaatggttt cttcgccggt
     7321 agcgatagtg cccgcgttaa tatccattaa atcaaaccag cggttcgcca gctcggtgcg
     7381 ggtcgccatt ttaatgaccg gtaccgccat caggccgtac ggcgtaccac gaccggtcgt
     7441 aaacacttgc acagtgatac ccgaagccac ctgttgcgtg ccacagacaa aatcgctggc
     7501 tggcgtcgcg gcgtaaatta atccgcgttt agtcgggcgt tggccgggcg acagcacttc
     7561 aacaattgca cttttacccg atttagcaat ggagccgagc gctttctcca ccacatttgc
     7621 cagaccgcct ttcttgttgc ccggcgaagg gttggcgctg cggtcggttt ttcccatatt
     7681 gagatagtta tcgtaccact ccatctcctc cagcagccgt ttgccgacct cttcgttcac
     7741 tgcgcgtggt gtcagcagat ggatcgcgtc acgcacttcc gtgacttctg aaaacatcac
     7801 cgttgcgccg cagcgcacca gtagatcaga cgcataccca accgccgggt ttgccgttac
     7861 gccagaaaac gcatcgctgc caccgcactg catgccgaca accagttctg aagccggaca
     7921 ggtttctcgc tgtcgttgat tcagtttgtg caaatggcgt tcggcgacct gcaaaatatc
     7981 ctcgaccatg gactgaaagc cgacatgctt ttcatcctgc aaactgacaa tgctggcgct
     8041 ttctactgga atagcttgca catcatccgt tccagtcagc aggcgctcag gctgcaactt
     8101 ttcacaaccc agaccaatca ccattacttc gccgccaaag ttagggttca gcgaaatatt
     8161 gtggatggta cgaataggca caactgccgc cggtgcgtta atcgccacgc cacagccgta
     8221 caaatgattc agccccacca cgccatcgac gttcgggtat ttcggtagca gatcgcgttc
     8281 aatgattttt actacatagt ccaccacgcc tgctacacag tggacgctgg tggtgatgcc
     8341 gagcaggttt ttagtgccca cgctgccatc ggcattgcga tagccctcaa aggtgtatcc
     8401 ttccagcggc ggtaagggtt ccgggacttt ggttgccagt ggcagcgtgt gtaacggcgg
     8461 cgcttccggt agtacaacca ttgattcgtc gatccagctt ccgcgtggga ttgcgcgcac
     8521 ggcgtaacca atcacttcgc cataacgaat aatttcacca ttagctggaa tgtccagcaa
     8581 tgcgacttta tgcccctggg gaatatgttc aattaattcc agcccatccg gaaaacgcgt
     8641 tcctgctttc aggccattat cattaacaat aattgccaca ttatctgtgt cgtgaacttt
     8701 tatataaaac gcagttggcg tttcttgtct gatttcgatg ttggccattg tgcaatattc
     8761 tccagccagg ggttattaaa atgaggacct ggtctcattt aaaaataaat gacttcattg
     8821 ggtgaaataa gaatgtcagg agtttaaacg cagaaatgtg atcaatgtca atcatttcag
     8881 ggattgcagt gccgcccggc aaggtaaagc gcaaatctgt gctttagcgc gcaaccttat
     8941 gtgcatatgc tcaaaataat atttaaatag tattaattaa attagcattt gcacaatgat
     9001 tattattctc gcgctgatta tactaattcc cgagttcatt gccgtaacgg cataaataaa
     9061 actcataaat aacaccttcc ccataataat aaaaatcagc ataagtaccc gaggtaaata
     9121 aaatgattct ggacaccgtt gacgtaaaaa agaaaggcgt gcatacccgc tatttaatat
     9181 tactgattat ttttattgtt accgccgtta actacgccga tcgtgcaacg ctgtctattg
     9241 ctggtaccga agtggcaaaa gagttgcagt taagtgcggt ttcgatgggt tacatcttct
     9301 ccgcttttgg ctgggcctac ctgctgatgc aaattcccgg cggctggctg cttgataagt
     9361 ttggctcgaa aaaagtttac acctacagcc tctttttctg gtcgctgttc accttcctgc
     9421 aaggctttgt tgatatgttc ccgctggcct gggcagggat ctccatgttc tttatgcgct
     9481 ttatgctggg gttctcggaa gcgccatcat tcccggcgaa cgcccgtatt gtcgccgcct
     9541 ggttcccaac gaaagaacgt ggtactgcct ccgccatctt taactcggcg caatatttct
     9601 cgctggcgct cttttcgccg ctgcttggct ggctgacttt cgcctggggc tgggagcacg
     9661 tcttcaccgt tatgggggtg attggttttg tgctgacggc gctgtggatc aagttgattc
     9721 ataacccgac agatcaccca cgtatgtctg cggaagagct gaagtttatc tcagaaaatg
     9781 gcgcggtggt cgatatggac cacaaaaagc cgggcagtgc ggcagcaagc ggtcccaaac
     9841 tgcattacat caagcaattg ctctctaacc gcatgatgct gggcgtattt ttcggacaat
     9901 attttatcaa caccatcacc tagttcttcc tcacctggtt cccaatttat ctggtgcagg
     9961 aaaaaggcat gtcgattctg aaagtgggtc tggtcgcctc gattccggca ctgtgtggct
    10021 ttgcgggcgg cgtactggga ggtgtcttct cggattatct gatcaaacgc ggtttgtccc
    10081 tgaccctggc acgtaagcta ccgattgtgc tgggaatgtt gctggcttcc accattatct
    10141 tatgtaacta caccaacaac accacgctag tggtcatgct gatggcgctg gctttctttg
    10201 gcaaaggatt tggtgcgctg ggctggccgg taatttctga caccgcgccg aaagagattg
    10261 ttggtctctg cggcggcgtc tttaacgtct ttggcaatgt tgcctccatt gtcactccac
    10321 tggtgattgg ctacctggta agtgaattgc actccttcaa tgcagcactg gttttcgtgg
    10381 gatgttcagc gctgatggcg atggtctgct acctcttcgt agttggcgac attaaacgta
    10441 tggaattgca gaaataagca aaggtataag cgatgaataa cgatgttttc ccgaataaat
    10501 tcaaagccgc actggctgcg aaacaggtac aaattggttg ctggtcagca ctctctaacc
    10561 cgattagcac tgaagtcctt ggtttggctg ggtttgactg gctggtgctg gatggcgaac
    10621 atgcgccaaa cgatatctcc acgtttattc cgcagttaat ggccctgaaa ggcagcgcca
    10681 gcgcgccagt agtgcgagtg ccgaccaacg agccggtaat tattaagcgt cttctggata
    10741 tcggtttcta taacttcctg attccttttg tagaaacaaa agaggaagca gagcaggcgg
    10801 tggcatcaac ccgttaccca ccggaaggca ttcgcggcgt ctccgtttct caccgcgcca
    10861 atatgtttgg caccgtggcg gattatttcg ctcagtcgaa caagaacatc accattctgg
    10921 tccagataga aagtcagcag ggcgtagata acgtcgatgc cattgccgct accgaaggcg
    10981 tagacggtat cttcgtcggc cccagcgatc tggctgcggc attaggccat ctcggcaatg
    11041 cctcgcaccc ggatgtacaa aaagcaatcc agcatatttt taaccgtgcc agtgcgcacg
    11101 gcaaacccag cggtatcctc gcaccggtcg aagccgatgc gcgtcgttat ctggaatggg
    11161 gggcgacgtt tgtggctgtc ggcagcgatc tcggcgtctt ccgctctgcc actcagaaac
    11221 tggctgatac ctttaaaaaa taaccaccac cgcaacaaga gagatgattg atatgactat
    11281 gaaagttggt tttattggcc tggggattat gggtaaacca atgagtaaaa accttctgaa
    11341 agcaggttac tcgctggtgg ttgctgaccg taacccagaa gctattgctg acgtgattgc
    11401 tgcaggtgca gaaacagcgt ctacggctaa agcgatcgct gaacagtgcg acgtcatcat
    11461 aaccatgctg ccaaactccc ctcatgtgaa agaggtggcg ctgggtgaga atggcattat
    11521 tgaaggcgcg aagcctggta cggtattgat cgatatgagt tctatcgcac cgctggcaag
    11581 ccgtgaaatc agcgaagcgc tgaaagcgaa aggcattgat atgctggatg ctccggtgag
    11641 cggcggtgaa ccgaaagcta tcgacggtac gctgtcagtg atggtgggcg gcgacaaggc
    11701 tattttcgac aaatactatg atttgatgaa agcaatggcg ggttccgtgg tgcataccgg
    11761 ggaaattggt gcaggtaacg ttaccaaact ggcgaatcag gtcattgtgg cgctgaatat
    11821 tgccgcgatg tcagaagcgt taacgctggc aaccaaagcg ggcgttaacc cggatttggt
    11881 gtatcaggca attcgcggtg gactggcggg cagtaccgtg ctggatgcca aagcgccgat
    11941 ggtgatggac cgcaacttca agccgggctt ccgtattgat ctgcatatta aggatctggc
    12001 aaatgcgctg gatacttccc acggcgtcgg cgcgcaactg ccgctcacag ctgcggttat
    12061 ggagatgatg caggcactgc gagcagatgg tttaggaacg gcagatcata gcgccctggc
    12121 gtgctactac gaaaaactgg cgaaagtcga agttactcgt taatgacgtc gtgcccggat
    12181 ggcgtattgc aatccgggcc tggaatccag gccgaataag agaaacgccc tccggcgtca
    12241 tgtggtaaca ggcataggta tgaaaatcgt aatcgcccca gactcttata aagaaagttt
    12301 atctgccagc gaggttgcgc aggcgataga aaaaggattt cgggaaattt ttcctgatgc
    12361 acagtacgtt tctgttcctg ttgccgatgg tggcgaagga acggtggaag cgatgattgc
    12421 agccacccag ggttccgaac gtcacgcctg ggttacaggg ccgctgggcg agaaggtgaa
    12481 tgccagttgg gggatctccg gcgatggcaa aaccgcgttt attgaaatgg cggcggccag
    12541 tgggctggag ctggtacctg cggaaaaacg tgatccactc gtgaccactt cacgcggcac
    12601 aggcgagtta attctgcagg cgctggagag cggcgcgaca aacattatta tcggcattgg
    12661 cggtagcgct acaaatgatg gcggtgcagg catggtacag gcgctggggg cgaaattatg
    12721 cgacgccaac ggcaatgaaa ttggttttgg cggcggtagt cttaatactc tgaatgatat
    12781 tgatatttcc ggcctcgatc cgcgcttaaa agattgcgtc attcgcgtcg cttgtgatgt
    12841 caccaatccg ctggtgggcg ataacggcgc atcgcgcatc tttggcccac aaaagggagc
    12901 cagtgaagcg atgattgttg agctggacaa taacctttct cactatgccg atgtcattaa
    12961 aaaagcgctg catgttgatg tgaaagatgt ccccggcgca ggagctgcgg gtggtatggg
    13021 cgcggcgcta atggcgtttc ttggtgcgga actgaaaagc ggtattgaaa tcgtcacgac
    13081 ggcgctgaat ctggaggaac atattcacga ttgtacgctg gtgatcaccg gtgaagggcg
    13141 tattgacagc cagagtatac acggcaaggt tccgattggc gtcgcaaacg tggcgaaaaa
    13201 gtaccataaa ccggtgattg gcattgcggg tagcctgacc aatgatgttg gcgttgtaca
    13261 tcagcatggc attgatgcgg tcttcagcgt attgaccagt attggtacgt tggacgaagc
    13321 attccgcggg gcttatgaca atatttaccg tgcttcacgt aatatcgccg cgacactggc
    13381 gattggaatg cgcaacgcgg ggtgacaagg gcgcgcaaac cctctatact gcgcgccgaa
    13441 gctgaccaga cagtcgccgc ttcgtcgtcg tcctcttcgg gggagacggg cggaggggag
    13501 gaaagtccgg gctccatagg gcagggtgcc aggtaacgcc tgggggggaa acccacgacc
    13561 agtgcaacag agagcaaacc gccgatggcc cgcgcaagcg ggatcaggta agggtgaaag
    13621 ggtgcggtaa gagcgcaccg cgcggctggt aacagtccgt ggcacggtaa actccacccg
    13681 gagcaaggcc aaataggggt tcataaggta cggcccgtac tgaacccggg taggctgctt
    13741 gagccagtga gcgattgctg gcctagatga atgactgtcc acgacagaac ccggcttatc
    13801 ggtcagtttc acctgtttta cgtaaaaacc cgcttcggcg ggtttttgct ttttggagga
    13861 gcggaaagat gaatgactgt ccacgacgct atacccaaaa gaaagcggct tatcggtcag
    13921 tttcacctgt tttacgtaaa aaaccgcttc ggcgggtttt tgctttttgg agcatagtgt
    13981 aagatgaata aatatctacg gtattaataa tagactttgt aagaaattat taagtctata
    14041 ttgcatttaa aaggtcgtaa tcatacgtat agtcctctct ggtaaaatat tgcttctctg
    14101 agttataagc ttcatatgtg ttatatttta cgacctcaaa gcaattgtca tgagaatttt
    14161 cgtctttctc tagtgtatta tctgaaagga attcctctga gctagaatcc tctatgttta
    14221 aatctgtatt gtaattaatt tttggaacga ttagataagc attttctttg acgctttcat
    14281 aatctttaat gattatatcg atgtaataat ctattttttc taatgagaat gctttcatct
    14341 ctggtaattt aattctgttc aagtaattgt taattttcga tgagctttta tatatttctt
    14401 ttgttaattc attttctaat tcatctgtta gagttgctct tggtatagat tctataattg
    14461 caagtctcat aatttttaat tgttcttcgg taatatctcc aagcgcattt aaacaacttt
    14521 cgacaagctt aattttttct tcagaataca tatcgttaat gcttgttata acaggcgtta
    14581 tttttagtaa ataatcccat gagattgtgg cgttttgttt tgaactaacg aaatttacct
    14641 gaaacatgtt ggcattagta aaatctgtgt catccaattt aaaaggattt aaaattgtat
    14701 tggccatatg tttatctgta aaatcataaa gttctaaatc gacattccgt aagtccagag
    14761 aggatagatc aactatacct ccagtcccac aactaaagat tacgttcaac ctcctgattt
    14821 cattgtaaat gtctttcaat gggcaattaa cattgatatt gcctgtggat atttcctggt
    14881 tttttaacgg ttcattaatt gtaagttcac aggtattgtc tgatcgttga ataattcggg
    14941 cgttataacc aaaaaaatcc atacgaagag acgaatcatt ataaagaatt acattttgta
    15001 agtacgctaa ttctttttga aaatcaccag tgataatgca tttcccacct ggttgtaacg
    15061 cgttaagtaa agtgaccatt tcaatagagg tctgttttag tttttctata cctggtgcta
    15121 gtgcttcgag gttattaact atggggcttt caggaagatc attcattttt cgacggacta
    15181 aatcactact aatatcatta ccaattgatg aaacagggaa cattatttta tcctttataa
    15241 ataatcaata aaaataatta ttaaaatgat cgggtgaaaa aaagagaatt tgccaacaat
    15301 ctgaagtaat gctggtttta ttaaagtgcg caggaggtgt taagatggct tcttttcggg
    15361 tgaattttgc acaatactta gaaatcgtct ccttaataca ttccagctct tttttattga
    15421 cggctgagca aatacattgt tcaccaaaag gtgcaaaagt gcctacatct aatgtttgta
    15481 tgatataatc gtcatgttct cctgtagaaa tatctatgat gaatggactc atattttccg
    15541 gttggattcg gatcatgaca gaaggaatat cgccttgata gaaattcaat gcagtaattg
    15601 cagataatac tctccgcagt tcattactta tactgtatat tttattaacg tcattggtgg
    15661 agacaatttc tgcaaacttt ctttttaccg tccaataatc aatatttctg ttgtgattgt
    15721 taaccactgc gtgcattggc gggattgaag ggtgaaaaat atgtgcaatt gggaagcctt
    15781 tcattgatgc ctgccttgtc atcatgacaa acaataatag atgcttattt tcctcgcacg
    15841 gcactttcgc actatcaaaa aacgctcatt tacccggcat tacagtaaac gaagcaacat
    15901 tctgtggcct acgccgatca aaagctctgt gaaaattccc cttgttgtct ttggctgacc
    15961 ttatcgcggc gaccagcaat tattcgtttt gtgatcctca acacaaaatg ctattcgtca
    16021 cctatgaggg gagaagaatt tatggaaaac gattgcattc acaaaatgac gtcctgtcat
    16081 gagggagaaa gttgacgtta atcaattgtt cgagatgatc aatacctgta ttcgaaattt
    16141 taaaatattg ataaaataaa tgtttgttct ccaacaaata gcgtgaaaaa atgtgacgtg
    16201 ccgcattatt tatcgctgaa aatggtttat ttataataaa atttgagaac ttgctcatta
    16261 aatttaactc aaattttgcc tggtaattat ccggtaattg cttgaaatat ggtctcagcc
    16321 ccttttttgt attaaccaca taacgaatga tgttatcgcc ataaaatatg gttatccccg
    16381 tcattttttt tgacaaaaat cagggtttat gctgattttt atactttaac ttattgatat
    16441 ataaagatat ttaattgaaa taacgatgcc ctggaatgta ttgaaagtta atttgtgagt
    16501 ggtcgcacat atcctgttca tttcattttg atacacttca tgccgtcaat gaggtaatta
    16561 acgtaggtcg ttatgagcac tattcttctt ccgaaaacgc agcacctggt agtctttcag
    16621 gaagtcatta gaagtggctc tatcggctcg gctgcaaaag aattagggtt aactcaaccg
    16681 gccgtcagta aaatcattaa cgatattgaa gattattttg gcgtggaatt agtggtgcgg
    16741 aaaaataccg gtgtaacatt aacacctgcc ggtcaattgt tactctcccg ttccgaatcc
    16801 attacccgtg aaatgaaaaa tatggttaat gagataagcg gtatgtcttc tgaggcggtg
    16861 gtggaagtct catttggttt tccttcattg attggtttta cttttatgtc agggatgatc
    16921 aacaagttca aagaggtgtt cccgaaagcg caggtttcta tgtatgaagc gcaactgtct
    16981 tcgttcttac cggcaatccg cgacggtcga cttgattttg cgattggtac gttaagcgca
    17041 gaaatgaagc ttcaggattt acatgttgag ccgctgttcg agtccgagtt tgagctggta
    17101 gccagtaagt cccgaacatg caccggcacc accacgctgg agtcgttgaa gaacgaacag
    17161 tgggtgttgc cacaaaccaa tatgggatac tacagcgagc tgctcaccac gttacaaaga
    17221 aatggcatca gtattgaaaa catcgttaaa accgactcag tcgtgacaat ttataatctt
    17281 gttctcaatg ctgatttctt aactgtaatt ccatgtgata tgacatcacc ttttggttct
    17341 aatcaattta ttactattcc ggttgaagaa acattacctg tggcacaata tgccgcggta
    17401 tggtcgaaaa attatcgtat taaaaaagca gcatcggttt tggtggaatt agccaaagag
    17461 tattcatctt ataatgggtg tagacgaagg caattaattg aagttggtta gttatttgtt
    17521 tttatttaaa cataaataat ccacctgtct gttttgcagg tgtcggttac ggttacctac
    17581 atatttaatt caggctaaga ggttttataa tgcatattac atacgatctc ccggttgcta
    17641 ttgatgacat cattgaagca aaacaacgac tggctgggcg aatttataaa acaggcatgc
    17701 ctcgctccaa ctattttagt gaacgttgca aaggtgaaat attcctcaaa ttcgaaaaca
    17761 tgcagcgtac gggttcattt aaaattcgtg gcgcatttaa taaattaagt tcactgaccg
    17821 atgcggaaaa acgcaaaggc gtggtggcct gttctgcggg caaccatgcg caaggggttt
    17881 ccctctcctg cgcgatgctg ggcatcgacg gtaaagtggt gatgccaaaa ggtgcgccaa
    17941 aatccaaagt agcggcaacg tgcgactact ccgcagaagt cgttctgcat ggtgataact
    18001 tcaacgacac tatcgctaaa gtgagcgaaa ttgtcgaaat ggaaggccgt atttttatcc
    18061 caccttacga tgatccgaaa gtgattgctg gccagggaac gattggtctg gaaattatgg
    18121 aagatctcta tgatgtcgat aacgtgattg tgccaattgg tggtggcggt ttaattgctg
    18181 gtattgcggt ggcaattaaa tctattaacc cgaccattcg tgttattggc gtacagtctg
    18241 aaaacgttca cggcatggcg gcttctttcc actccggaga aataaccacg caccgaacta
    18301 ccggcaccct ggcggatggt tgtgatgtct cccgcccggg taatttaact tacgaaatcg
    18361 ttcgtgaatt agtcgatgac atcgtgctgg tcagcgaaga cgaaatcaga aacagtatga
    18421 ttgccttaat tcagcgcaat aaagtcgtca ccgaaggcgc aggcgctctg gcatgtgctg
    18481 cattattaag cggtaaatta gaccaatata ttcaaaacag aaaaaccgtc agtattattt
    18541 ccggcggcaa tatcgatctt tctcgcgtct ctcaaatcac cggtttcgtt gacgcttaat
    18601 taattcgttg aggataggat atgagtactt cagatagcat tgtatccagc cagacaaaac
    18661 aatcgtcctg gcgtaaatca gataccacat ggacgttagg cttgtttggt acggcaatcg
    18721 gcgccggggt gctgttcttc cctatccgcg caggttttgg cggactgatc ccgattcttc
    18781 tgatgttggt attggcatac cccatcgcgt tttattgcca ccgggcgctg gcgcgtctgt
    18841 gtctttctgg ctctaaccct tccggcaaca ttacggaaac ggtggaagag cattttggta
    18901 aaactggcgg cgtggttatc acgttcctgt acttcttcgc gatttgccca ctgctgtgga
    18961 tttatggcgt tactattacc aataccttta tgacgttctg ggaaaaccag ctcggctttg
    19021 caccgctgaa tcgcggcttt gtggcgctgt tcctgttgct gctgatggct ttcgtcatct
    19081 ggtttggtaa ggatctgatg gttaaagtga tgagctacct ggtatggccg tttatcgcca
    19141 gcctggtgct gatttctctg tcgctgatcc cttactggaa ctctgcagtt atcgaccagg
    19201 ttgacctcgg ttcgctgtcg ttaactggtc atgacggtat cctgatcact gtctggctgg
    19261 ggatttccat catggttttc tcctttaact tctcgccaat cgtctcttcc ttcgtggttt
    19321 ctaaacgtga agagtatgag aaagacttcg gtcgcgactt caccgaacgt aaatgttccc
    19381 aaatcatttc tcgtgccagc atgctgatgg ttgcagtggt gatgttcttt gcctttagct
    19441 gcctgtttac gctgtctccg gccaacatgg cggaagccaa agcgcagaat attccagtgc
    19501 tttcttatct ggctaaccac tttgcgtcca tgaccggtac caaaacaacg ttcgcgatta
    19561 cactggaata tgcggcttcc atcatcgcac tcgtggctat cttcaaatct ttcttcggtc
    19621 actatctggg gacgctggaa ggcttgaatg gcctgattct gaagttcggt tataaaggtg
    19681 acaaaaccaa agtgtcgctg ggtaaactga atactctcag catgatcttc atcatgggct
    19741 ccacctgggt tgttgcctac gccaacccga acatcctcga cctgattgaa gccatgggcg
    19801 caccgattat cgcatccctg ctgtgcctgt tgccgatgta tgccatccgt aaagcgccgt
    19861 ctctggcgaa ataccgtggt cgtctggata acgtgtttgt taccgtgatt ggtctgctga
    19921 ccatcctgaa catcgtatac aaactgtttt aatccgtaac tcaggatgag aaaagagatg
    19981 aatgaatttc cggttgtttt ggttattaac tgtggttcgt cttcgattaa gttttccgta
    20041 ctcgatgcca gcgactgtga agtattaatg tcaggtattg ccgacggtat taactcggaa
    20101 aatgcattct tatccgtaaa tgggggagag ccagcaccgc tggctcacca cagctacgaa
    20161 ggtgcattga aggcaattgc atttgaactg gaaaaacgga atttaaatga cagtgtggcc
    20221 ttaattggcc accgcatcgc tcacggcggc agtattttta ccgagtccgc cattattacc
    20281 gatgaagtca ttgataatat ccgtcgcgtt tctccactgg cacccctgca taattacgcc
    20341 aatttaagcg gtattgaatc ggcgcagcaa ttatttccgg gcgtaactca ggtggcggta
    20401 tttgatacca gtttccacca gacgatggct ccggaagctt atttatacgg cctgccgtgg
    20461 aaatattatg aagagttagg tgtacgccgt tatggtttcc acggcacgtc gcaccgctat
    20521 gtttcccagc gcgcacattc tttgctgaat ctggcggaag atgactccgg cctggttgtg
    20581 gcgcatcttg gcaatggcgc gtcaatctgt gcagttcgca acggtcagag tgttgatacc
    20641 tcaatgggaa tgacgccgct ggaaggcttg atgatgggta cccgcagtgg cgatgtcgac
    20701 tttggtgcga tgtcctgggt cgccagccaa accaaccaga gcctgggtga cctggaacgc
    20761 gtagtgaata aagagtcggg attattaggt atttccggtc tttcttcgga tttacgtgtt
    20821 ctggaaaaag cctggcatga aggtcacgaa cgcgcgcaac tggcaattaa aacctttgtt
    20881 caccgaattg cccgtcatat tgccggacac gcagcctcat tacatcgcct ggatggaatt
    20941 atattcaccg gcggaatagg agagaattca agtttaattc gtcgtctggt catggaacat
    21001 ttggctgtat taggcgtaga gattgataca gaaatgaata atcgctctaa ctcctgtgga
    21061 gagcgaattg tttccagtga aaatgcgcgt gtcatttgcg ccgttatccc aaccaacgaa
    21121 gaaaaaatga ttgctttgga tgccattcat ttaggcaaag tgaacgcgcc cgcagaattt
    21181 gcataattta gttgaagtat tgtagagaga ttatttttca tgaaggtaga tattgatacc
    21241 agcgataagc tgtacgccga cgcatggctt ggctttaaag gtacggactg gaaaaacgaa
    21301 attaatgtcc gcgattttat tcaacataac tatacaccgt atgaaggtga tgaatctttt
    21361 ctcgccgaag caacgccagc caccacggaa ttgtgggaaa aagtgatgga aggcatccgt
    21421 atcgaaaacg caacccacgc gccggttgat ttcgatacta atattgctac caccattacc
    21481 gctcatgatg cgggatatat taaccagccg ctggaaaaaa tagtgggcct gcaaacggat
    21541 gcgccattaa agcgtgcgct gcatccgttc ggtggcatta atatgattaa aagttcattc
    21601 cacgcctatg gtcgtgaaat ggacagtgaa tttgaatatc tgtttaccga tctgcgtaaa
    21661 acccataacc agggcgtatt tgatgtttac tcaccggata tgctgcgctg ccgtaaatct
    21721 ggcgtgctga ccggtttacc agatggctat ggtcgtgggc gcattatcgg tgactatcgc
    21781 cgcgtggcgc tatatggcat cagttatctg gtgcgtgaac gcgaactgca atttgccgat
    21841 ctccagtccc gtctggaaaa aggcgaggat ctggaagcca ccatccgtct gcgtgaggag
    21901 ctggcggaac atcgtcgtgc gctgttgcag attcaggaaa tggcggcgaa atacggtttt
    21961 gatatctccc gtccggcgca gaatgcgcag gaagcggtgc agtggctcta cttcgcttac
    22021 ctggcggcag tgaaatcaca gaacggcggc gcaatgtctc ttggccgtac ggcatcgttc
    22081 ctcgatatct acattgagcg cgactttaaa gctggcgtac tcaatgaaca gcaggcacag
    22141 gaactgatcg accacttcat tatgaagatc cgtatggtgc gcttcctgcg tacgccggaa
    22201 tttgattcgc tgttctccgg cgacccaatc tgggcgacgg aagtgatcgg cgggatgggg
    22261 ctggacggtc gtacgctggt gaccaaaaac tctttccgtt atctgcacac cctgcacact
    22321 atggggccag caccggaacc aaacctgacc attctgtggt cggaagaatt accgatcgcc
    22381 ttcaaaaaat atgccgcaca ggtgtctatc gtcacctctt ccttgcagta tgaaaacgac
    22441 gatctgatgc gtactgactt caacagcgac gattacgcga ttgcctgctg cgttagcccg
    22501 atggtgattg gtaagcaaat gcagttcttt ggtgcacgcg ctaacctggc gaaaacgctg
    22561 ctctacgcaa ttaacggcgg ggtggatgag aagctgaaga ttcaggtcgg gccgaaaaca
    22621 gcaccattga tggacgatgt gctggactac gacaaagtga tggacagcct cgatcatttc
    22681 atggattggc tggcagtgca gtacatcagc gcgctgaata tcatccacta catgcacgac
    22741 aagtacagct atgaagcctc gctgatggcg ctgcacgatc gtgatgttta tcgcacgatg
    22801 gcatgcggca tcgcgggcct gtcggtggcg acggactccc tgtctgccat caaatatgcc
    22861 cgcgtgaaac caatccgtga cgaaaacggc ctggcggtgg actttgaaat cgacggcgaa
    22921 tatccgcagt acggtaacaa cgacgagcgc gtagacagca ttgcctgcga cctggttgaa
    22981 cgctttatga agaaaattaa agcgctgcca acctatcgca acgccgtccc tacccagtcg
    23041 attctgacta tcacttctaa cgtggtgtac ggccagaaaa ccggtaacac gccggacggt
    23101 cgtcgcgccg gaacaccgtt cgcaccgggc gctaacccga tgcacggtcg tgaccgcaaa
    23161 ggtgcggtag cctcgctgac gtcggtggcg aaactgccgt tcacctacgc caaagacggt
    23221 atttcgtaca ccttctcaat tgtcccggcg gcgctgggca aagaagatcc agtacgtaaa
    23281 accaaccttg tcggcctgct ggatgggtat ttccaccacg aagcggatgt cgaaggcggt
    23341 caacacctca acgtcaacgt aatgaatcgg gaaatgctgc tggatgccat cgagcacccg
    23401 gaaaaatatc ctaacctgac aatccgtgtc tctggctacg ccgtgcgctt caacgcactg
    23461 acccgtgaac agcagcagga tgttatttca cgtaccttta cccaggcgct ctgacgccgg
    23521 aggatgtatg aaaaagatta tcgaaacgca acgtgcccca ggcgcaatcg gcccttatgt
    23581 tcaaggcgtt gatttaggca gcatggtctt cacctccggg caaattccgg tttgcccaca
    23641 gaccggtgag atcccggctg atgtgcaaga tcaggcgcgt ttaagcctcg aaaacgtcaa
    23701 agcgatcgtg gttgctgccg ggctgagcgt gggcgatatc atcaagatga ccgtgttcat
    23761 caccgatctg aatgattttg ccaccatcaa tgaagtctat aagcagttct tcgatgagca
    23821 tcaggcgacc tacccgaccc ggagctgtgt gcaggtcgcg cgtttgccga aagatgtgaa
    23881 gctggaaatt gaagccatcg cagtacgtag tgcgtaaagc ctcgtgagcg ggacggtcgc
    23941 aaggtcgttc cgctccactt cactgaacgg caatccgagg gtgtggatat gattagtgca
    24001 ttcgatattt tcaaaattgg gattggtccc tccagttcgc ataccgtggg gccaatgaat
    24061 gccggaaaaa gttttattga tcggctggaa agtagcggct tattaaccgc gacgagccat
    24121 attgtggtcg atctgtacgg gtcgttgtca ctgacgggca aaggccatgc cacggatgtc
    24181 gccatcatca tgggactggc aggaaacagt ccgcaggatg ttgtcattga tgagatccct
    24241 gcatttatag agttagtgac gcgcagcggg cggctgccag tggcatctgg tgcgcatatt
    24301 gttgattttc ctgtagcaaa gaacattatc ttccatcccg aaatgttgcc tcgccatgag
    24361 aacggaatgc ggatcactgc ctggaaggga caggaagcgc tattaagcaa aacctattac
    24421 tctgtcggcg gcgggtttat tgtcgaagaa gaacacttcg gcctgtcgca cgatgtcgaa
    24481 acgtccgtac cttacgattt ccactcagca ggagaactgc tgaaaatgtg tgattacaac
    24541 ggcctgtcta tttctggcct gatgatgcac aacgagctgg cgctgcgcag taaagcggaa
    24601 attgatgccg gttttgcccg tatctggcaa gtgatgcatg acggtattga acgcgggatg
    24661 aacactgaag gcgtgctgcc tggtccgctc aatgtgccgc gccgtgccgt agcgctgcgt
    24721 cgtcagttgg tttccagcga taacatctct aacgatccga tgaatgtcat cgactggatc
    24781 aacatgtacg cgctggcggt tagtgaagaa aacgcagctg gcgggcgcgt ggtaacggca
    24841 ccgactaacg gtgcgtgcgg cattattccg gcagtgctgg cttattacga taagttccgt
    24901 cgtccggtaa atgagcggtc aattgcccgc tattttcttg cagccggggc tattggcgcg
    24961 ctgtataaaa tgaacgcctc catctctggc gcggaagtcg gctgtcaggg ggagattggc
    25021 gtggcctgtt caatggcggc ggcagggtta actgaactac tgggcggcag tccggcgcag
    25081 gtatgcaatg cggcggaaat cgcgatggag cataaccttg ggctgacctg cgatccggtt
    25141 gccggacagg tacaaatccc gtgcattgaa cgtaatgcca ttaatgccgt gaaagcagta
    25201 aacgccgcgc ggatggcgat gcgccgcacc tcggcaccgc gtgtttcact cgataaagtg
    25261 atcgagacga tgtatgaaac cggcaaagat atgaacgata aataccgcga aacatcacgc
    25321 ggaggactgg ctattaaagt ggtctgcggc tgacattgct gtcagctttc atccttgggt
    25381 gcacaaatgt gcaccttttt tttgtgatct gccgcaaata gagaatgaat attctcttgc
    25441 gattaattta gtagaaaatt cttatccatc tccgttattt caaagtcttg attgtgaaga
    25501 ttttctctga cctcgcgcgt atcatttctt tattctaata actcgcctgc aaaaacgtag
    25561 accgcatatc gccttgatgc ggctcggaaa aaaataataa gccctacaaa atattgagcg
    25621 agaaattatg gaaattgcat cgaataaagg cgtcattgca gacgcttcga ccccggcggg
    25681 tcgtgctgga atgagtgaga gcgagtggcg agaagcgatc aaattcgaca gtactgacac
    25741 cggctgggtg attatgagta tcgggatggc gattggcgcg gggattgttt ttctcccggt
    25801 gcaggtcggt ttgatggggt tgtgggtatt tttgctctca tcggtgattg gttacccggc
    25861 aatgtatctg tttcagcggt tgtttattaa tacgttggca gaatcaccag aatgtaaaga
    25921 ttacccgagc gtcattagcg gttatttagg taaaaactgg ggcatcctgt taggtgcact
    25981 ctatttcgta atgctggtga tttggatgtt cgtctattcc accgccatca ccaacgatag
    26041 tgcttcctac ctgcatacct tcggcgtgac ggaagggttg ctgtcagaca gtccctttta
    26101 tggtctggta ctgatttgca ttctggtggc gatctcttca cgcggcgaga aattgttatt
    26161 caaaatttcg accggcatgg tgctgaccaa gctgctggtg gtcgcggcgc tgggcgtatc
    26221 gatggtggga atgtggcatc tgtataacgt cggttcgcta ccgccgctgg ggctgctggt
    26281 gaaaaacgcc attattacgc tgccgtttac cctgacgtcg attctgttta tccagacgtt
    26341 aagcccgatg gtgatctctt atcgctcgcg ggaaaaatcg attgaagtgg cgcggcataa
    26401 agcattgcgg gcaatgaata tcgcgtttgg cattttgttt gtcaccgtct ttttctacgc
    26461 agtgtcgttc acgctggcga tgggacatga cgaagcggta aaagcctacg agcagaatat
    26521 ttccgcgctg gcgattgcgg cgcagtttat tagcggtgac ggcgcagcgt gggtgaaagt
    26581 ggtcagcgtc attctcaata tctttgcagt aatgaccgcg ttttttggcg tctatttagg
    26641 ctttcgcgaa gcaacgcaag ggatcgtaat gaacatcctg cgtcgcaaga tgcctgccga
    26701 gaagattaac gaaaatctcg ttcagcgcgg catcatgatt ttcgccattt tgctggcctg
    26761 gagcgccatc gtactgaacg caccggtgtt gagcttcacc tctatctgta gcccgatttt
    26821 cggcatggta gggtgcctga tcccggcgtg gctggtttac aaagtaccgg cattgcacaa
    26881 atacaaaggg atgtctctgt acctgattat cgtcactggt ttgttgcttt gtgtttctcc
    26941 gttcctggca ttttcttgat ttccctgaaa ttttaaggtt tttaatatgt ttgattcgac
    27001 tttaaatccg ttatggcagc gttacatcct cgccgttcag gaggaagtaa aaccggcgct
    27061 gggatgtact gaaccgattt cactggcgct ggcggcggcg gttgctgcgg cagaactgga
    27121 aggtccggtt gaacgtgtag aagcctgggt ttcgccaaat ctgatgaaga acggtctggg
    27181 cgtcaccgtt cccggcacgg gaatggtggg gctgccgatt gcggcggcgc tgggagcgtt
    27241 aggtggaaat gctaacgccg ggctggaagt gctgaaagat gcaactgcgc aggcaattgc
    27301 cgatgccaaa gcactgctgg cggcggggaa agtctcagtt aagatccagg aaccttgcga
    27361 tgaaatcctc ttctcacgcg ccaaagtctg gaacggtgag aagtgggcgt gtgtcaccat
    27421 tgtcggcggg cataccaaca ttgtgcatat tgagacgcac aatggtgtgg tgtttaccca
    27481 gcaggcgtgt gtgacagagg gcgagcaaga gtcgccgctg acggtgcttt ccaggacgac
    27541 gctggctgag atcctgaagt tcgtcaatga agtcccgttt gcggcgatcc gctttattct
    27601 cgattccgcg aagttaaatt gcgcgttatc gcaggaaggc ttgagcggta actgggggct
    27661 gcatattggc gcgacgctgg aaaaacagtg cgcgcgcggc ttgctggcga aagatctctc
    27721 ttcatccatt gtgattcgta ccagcgcggc atccgatgcg cgtatgggcg gcgctacgct
    27781 tccggcaatg agtaactccg gctcgggtaa ccaggggatc actgcaacaa tgcctgtggt
    27841 ggtggtagca gaacacttcg gggccgatga tgaacggctg gcgcgtgcgc tgatgctttc
    27901 gcatttgagc gcgatttaca tccataacca gttaccgcgt ttgtctgcgc tttgtgccgc
    27961 aacgaccgca gcaatggggg ccgccgccgg gatggcatgg ctggtggatg ggcgttatga
    28021 aaccatttcg atggcgatca gcagtatgat cggcgatgtc agcggcatga tttgcgatgg
    28081 tgcgtcgaac agctgcgcga tgaaggtttc gaccagtgct tcggctgcgt ggaaagcggt
    28141 gttaatggcg ctggatgata ccgcagtgac cggcaatgaa gggattgtgg cgcatgatgt
    28201 tgagcagtcg attgccaacc tgtgtgcgtt agcaagccat tcgatgcagc aaacggatcg
    28261 gcagattatc gagattatgg cgagcaaggc cagataatcg ccaggttttg gggagcgtgc
    28321 cggatgcgat gctggcgcat cttgtccggc ctacggggtg cgcaatttgt tgatgttgtt
    28381 gtgttttgta ggccggataa ggcgtttacg ccacatccgg cacttgtctt tacgcggtag
    28441 cttcatcctc ggctaaacgc gtttcagcat ccgcgacgat ggcttccagc tcgctcaaca
    28501 tctcttcata gccaaaattt ttggcggtgc gagaaggttc tttcgccacc acgggcttca
    28561 ctggctgacg ctttttggcc gattttttac tcatcatcat ctccctgtaa aaacaactag
    28621 acaggcaaat ctatcagcaa agcgcgcagt ggggaatcgg caaccagcgt aatgttagcc
    28681 tcgtcacgaa taaacgcccc atcaccgcag gtcagcgccg ctttctcttc atgatgcgta
    28741 agcgcatgaa atttcccgtg aatcgattgc aaatacgcgc gtggcccatg caactggaaa
    28801 ttcgcacttt cgcctttgtc gagcacgata tggtgcagcc acacttgctg gcgcaattgc
    28861 aggcttccca tcccaccttc tggcgaggcg attaattgct gcttgctcat attaagcgcc
    28921 agtttttgaa tcagcggatt ctctcgttgt gggcaggcat ccagccaaag ttgcattcgc
    28981 gttaacgatt tgtctttgct gagattgtgt tcgctatagc tgacacccgg ctgggtagag
    29041 agcagcaaca cctcaccggc gctggcctga acatgattgc cttcgctgtc gcgatactca
    29101 gcttctccat ccagaatcac atttaaaata tcgactttgg gataggttcg cggctgaaag
    29161 gcggcacctg gggccagcac ttcctggtta agcacgcgca gggaggcata gcctaacaat
    29221 ttcgggtcga agtagtgtcc aaaggaaaaa gtataccggg cctgcaacca tccgtagtct
    29281 gcttgtccac actgcctggc agttcgggta gtaatcataa ttcttgacct ctctttactt
    29341 ccttttatgg taaggggctg gacgctacat tgttagccag atattctgcc cggtatgttc
    29401 aaatttcctg aatgagaacg aaatggccaa agaaagggca ttaacgctgg aagcactacg
    29461 ggttatggat gcgatcgatc gccggggcag ttttgcggcg gcggcggatg agctgggacg
    29521 cgtgccttcc gcacttagct acaccatgca aaaattggaa gaagagctgg atgtggtgct
    29581 gtttgaccgc tcgggccatc gcaccaaatt caccaatgtc gggaggatgt tgctggagcg
    29641 agggcgcgtt ttgctggaag ccgcagataa actgactacc gatgcggaag ctctcgcgcg
    29701 cggttgggaa acgcatctca ccattgtgac cgaagcgctg gtaccgacac ctgccttttt
    29761 cccgttaatc gacaaactgg cggcaaaagc caatacccaa ctggcaatta tcaccgaagt
    29821 gctggcgggg gcgtgggaac ggctggagca ggggcgggcg gatattgtta tcgcgccgga
    29881 tatgcatttt cgctcctcgt cggagatcaa ctcgcgcaag ctctatacgt taatgaacgt
    29941 ctacgttgcc gcgcctgatc acccgattca tcaggagccg gaaccgttat ctgaagtgac
    30001 gcgcgtgaaa tatcgtggaa ttgcggtggc ggataccgct cgtgagcgcc cggtattgac
    30061 cgtacagcta ctggacaaac agccgcgttt aacggtgagc acgattgaag ataaacgtca
    30121 ggcattactg gcggggcttg gcgtggcgac gatgccgtat ccgatggtcg aaaaagatat
    30181 tgcggaaggg cggttgcgtg tcgtcagccc ggaatcgacc agcgagatcg atattattat
    30241 ggcctggcgt cgtgacagta tgggggaggc gaaatcctgg tgtctgcggg aaattcccaa
    30301 actttttagc ggaaaataat atttgttatt tttaataaaa aggggcgaaa tatcgcccca
    30361 attctaagat taatttgaac caaacttcgg atcgtttccg taacgattgc tgccagaatt
    30421 gccttctaaa caggcaaata caaacagaac taaccagcca atgaccggta ccagatagag
    30481 aagcgcccac gcacctgaac ggtcagtatc gtgcagacga cgaacgcaca gagcaatgac
    30541 cggaatgatt gttgctgcca ggtaaatgag agaaaggaat ggaaactcta aacctaaaat
    30601 caattgaatg acattgataa tagcgccgac gatggcgtta atcagagtaa acatccagta
    30661 ctctttacga cgtgcgcgac cagagaaacc aacataattt tttaatacgg ctaaatacca
    30721 ctgcataaaa acatcccttt aattaaaagt gacgaaagtc tattccgggt attgtgtaaa
    30781 tgctattcgt atctttcttt ttcggatgtt ataaaggttt tgatatttaa gataaataca
    30841 tggttatttt ttataggtaa tttatataac gatattgaaa tattttaaat aaacattagt
    30901 aaattattca ttcatcaaca tgatgataaa agccggatga gtcagacatc cggcgaggag
    30961 atttgtttta aggttccagt tttggatcag gtccaaagcg gttttcgcct ggcgtacccg
    31021 cctggcagtt aaagacgatg ataataagcc agccgataaa cgggattaag aacagcagtg
    31081 cccaccacgc cgagcggtcg gtgtcgtgca ggcggcggaa ctgaactgcc caccatggta
    31141 aaaacaccag aataccgtag attgtcgtca ggatgccttc gccaccggca cgttgccagc
    31201 ctaacatttt atccagtagc cccagcacga acgtaaagat gatgttgacc agaataaaca
    31261 tccagtactc tttgcgccgc gcacgacccc ggaaaccgac ataatttttt agtactttca
    31321 gataccagtc catttttgcc tcaattttgc tcaatcactt gtttttaaag cagcaaatta
    31381 agaatagacg tgaatgaaat ggcagtaaat attatctcta tgaattaata aggcgctgtg
    31441 aaggataggg gcaagcgcaa aattgccaga tgcgctacgc ttatctggcc tgcgcggtca
    31501 ttgcaacata ttgaatataa atgttttgta ggtttgataa ggcgtttacg ccgcatccga
    31561 catgaacaac atgcacttag ctaattatca gaggcacatc gtaaggtgcc tttttttaac
    31621 cgaagcgaac atctcgtcca tgcggttcat cgagatcctg ccacggacca attgaaataa
    31681 tccccgtagg gttgatggtc ttatggctgc ggaagtaatg attacggatg tgatcgaaat
    31741 tgactgtttc ggcaattccc ggcatctggt agatatcgcg caggaagcca tacagattca
    31801 ggtaatcgct gatgcggtgc ttatcacact tgaagtgggt cacatacact ggatcaaaac
    31861 gcaccagcgt ggtccacagg cgaatatcgg cttcggttag ctggttgccg gtcaggtaac
    31921 ggtgctgacc taaaatctgt tccagtcgcg ccagcgattc aaacactttc gccaccgcct
    31981 cgtcgtaagc ttgctggctg gtggcaaaac cggctttata cacgccgttg ttaacagtgt
    32041 cataaatcca gccgttaagt tcgtcaattt tcgtttgcag ggctggtggg tagtaatcac
    32101 ccgctttcgc gcccagcgca tcaaacgcgg tattgaacat gcgtatgatt tctgctgatt
    32161 cgttgctgac gatggtgtgg ttctttttgt cccacagcac gggaacagtc actcgtccgc
    32221 tgtagtgtgg atcggcgtgg agataaagct gatacagaaa ttcatgttga tagagcgtgt
    32281 cgccggttgc tcccggaaaa ctgtcatcaa aggtccagcc gttttccagc atcagcgggt
    32341 tcactacgga aacggaaata aacggttcca gtcctttgag tttacgcatg atcagcgtgc
    32401 ggtgcgccca cgggcaggcg agtgaaacat agagatgata acgatctttc tctgcgataa
    32461 aaccgccttt gccagtgggg cctggcgcgc catcggcagt gagccagtta cgaaatgcgg
    32521 aagctgaacg ttgaaattta ccgccggtag atttggtatc gtaccaggtg tcatgccaga
    32581 cgccgtcaat cagttgaccc atttttctct cctccaatag caaaaacgag gagcgtttct
    32641 cctcgtttga gactgatggc aaacgcaaaa ctgcctgatg cgctatgctt atcaggccta
    32701 cgaggtttct gcaatgtatt gaatttgcat gtttttgtag gccggataag gcgttcacgc
    32761 cgcatccggc atgaacaacg cgcactttgt caacaatctg aaacgaggag tgttcctcct
    32821 cgttttttat tgttaagtat agcgcgtgct taccactttt tattcagcag gcggtcgatg
    32881 ctatacgcgc ctggaccggt aattgccagc agcaggaatc cgccagaaat cgtcaggttt
    32941 ttcatgaaca tcagcgagtt gacgccttca gcaaagttgc tgtgaaataa aaatgccgtc
    33001 agcagcgtaa agcccgcagt aaacagggct gtggtacgtg tcaggaaacc gaacaggatt
    33061 gccagaccac caccaaactc aagcagaatc accagcggca gcataaagcc ggggacgccc
    33121 attgcttcca tatattgttg ggtacccgcg tagccagtaa tttttcccca gcctgcggta
    33181 ataaacagaa tcggcattaa aatacgcgct accagtacac caacatcttc taattttttc
    33241 atcatactct ccactgcgcc gctcaggctg ctgattgttt ttttgtgcaa gggcgcgcta
    33301 ttagcgtcgt tgctgtcgat ggagagaatc ataaacgtgg cgaagcgtga ttgttagcaa
    33361 ggaaaattgt caaaaatctt caaaaaattt gagggataag gccggaatgg ctccggccag
    33421 agggaagtta accgcgaagc tgttgctgct tgagggtcgt tttaaccaga cgccaggcgc
    33481 tccatacgcc aaaaccgcgt ctggcccagc ggaccagcat attaggatgg cgaatcgtcc
    33541 agatcgccat cacgctactg ccaaccagcg cccaggagcg cagacttagc agcatattcc
    33601 agcgacgatc gtaagcgcct gttgcctcca gccattcacg acgactggca gaaagatcca
    33661 gccgttgttg ctggatctgg ctaagcagtt gcgccttacg tcgttcacgt tcgactttac
    33721 tgctcacggg actcctcctc gagcagctgc cgatcgtttg ccagctcatg gcgtgtatgg
    33781 cgcagcaagg tggatttacg cgatttacgt aacgtccaga taccgccaat cagtgccagt
    33841 agcagcaaca ccacggtggt ggcaatcatc gcattcaggc gatattgcgg atcaaccgcc
    33901 caaataatta gcaccatcag gctcataaga ccaaatgcag cgaaaagcat cgtcaggccc
    33961 agcatcagta aaagttgaaa gagattcgct ttttcctctt ccagctccac caccgccagc
    34021 cgcagacgtg tttccaccat ttcaaccatg atggaaacaa ttcgctgccc gatgcccaga
    34081 acgcttttac cgggcccttg cgcgtgatga gtgtccgcca taattaacga cgcgacagca
    34141 gaacgccgag cactacaccg attgcagcgc caatgcccac gccggtccac ggattttcac
    34201 gcacatactc atcggcacgc gccgccgcga cacgggtttg tttggcgatg gcatcaccgg
    34261 tttcacccag gcgataacgg ctctgtttca gtgcctgctc cgctttgcta cgaatcttac
    34321 tcaactcttc tttcgacttc tcgccagatg agctaagcac ctcttccagc gtatcggaaa
    34381 gggatttcaa ctcagcacgc agatgttccg tagtgtgttc tttcgacata gttttctcca
    34441 ggtgagtaag tagtgactat tgtgagttag tagtcgcgcg cttccagctt tttcagctct
    34501 tcctgcgctt ctgccagttt ccgttcgcgt ttggcaatct tatctgcatc gcctttttgc
    34561 ttcgcctcgg ctaaatcttg ctggcgttcc gccacctcat ctttctgctt tgcgattttc
    34621 ttctgatgat cggcacgcag ttggctatct gaacagttgg cccggacttc actcagggct
    34681 ttattcagac cgtcaatacg attctggttt tggtgttttt cggcatagct gatctcctta
    34741 aggatatttt gctccttttc ctgacacagg gtagtggcat aactaccggc actaagagca
    34801 aagagagaaa cagctaaagc gatgcggtat ttcattcttg aaccttccat tgtgattcgg
    34861 acctattaca aaggaacgat tgtccagaca acgaagcggt cgtgttaacc accgccccat
    34921 taatcataga cagttacggt gaaatcacca aagtgagtga tctgtttcgg ataacaggaa
    34981 gttatccgaa gcgatgagag ttatcccgca accgggtcag ccactgcatt gcctgactat
    35041 tattgtcctg ctgcgcaatg atgtagccat ggggcaatgt tctgtcgagg accgcttttg
    35101 cggcggcgct ctggtcgctg gagtcaaagg taattaacag agtgtcgttt ttgggggtga
    35161 tacttttgaa aggaatgccg tgagcgtcaa ggtgatgcca gattgaaaaa ccgtctggca
    35221 tcgttgtgcc ttgatgaacg gcacgaatcg ccagcgtaga ctcttgctgg cgaaccgcgg
    35281 accaggccag caacagcgtg ccgaccagaa gtaaaacagc gccggaccag gctagctggc
    35341 gaagtgacat gcgaggtatt tgcatccctt acccccgatt tccatatttc tttttccata
    35401 acacaaccag agaacctgcc aggccaaaca ccagcagcac caccgggagc agcatcaggc
    35461 atgacatcag ctggtcctcg tactttaaaa ataccggcgt tttgccgagc atgtaaccca
    35521 gagttgtcag gatcaatacc cacagcagac cactcatcca gttgaagaac tgaaagcgcg
    35581 cgttattcag ccctgataac ccggcaatcg tcggcagcag tgttctgaca aacgcaataa
    35641 agcgaccaat taacagcgcc gataaaccgt gtttatgaaa aagatggtgt gcgcgttgat
    35701 gataatgcgc gggtaaatga gatagccagt tttgtacggt gcgggtattg cccagccatc
    35761 gcccctgaat atagctgacc cagcagccga ggctggcggc aacggtcagc agcagaatcg
    35821 tttgcggata gcccatcgcg cctttcgcaa tcaatacgcc gaccagtacc agtaaactgt
    35881 cgcccggtaa aaaggccgcc ggaagcaagc cgttttcaag gaacaaaatg acaaacaaga
    35941 caaaatacaa catgccaatc atcgacggat tggccagggt ttcaaaatcc tgcgcccaca
    36001 gggcttgcag caattgggtc aaaagttcca ttcattattc ctggtgaatc ggttaacgcc
    36061 acgctggttt taaatgatgc actaccgcaa tgttattgct gttttggtat gttttgcggg
    36121 tttgatacat caacacagcc tgaactattc cctgttaaat cgtgagataa gaaagcattc
    36181 aaaagcataa attcaaattg aatctaattg taacaaaggt caacgttgtt cgttatcaaa
    36241 attacatgtc cgtggagtct gattttgctg aatgcagggg agggaggcga gaggatttac
    36301 acaggctgac actttatact tttcgcttac cgacgagaca aacaggcgca ctgtagcgcc
    36361 tgtcggacag gaatttcatt tactgccgct ggtggctgta tcgagatgca ccaccgggtt
    36421 ttcggcgaac agatagcggt cggcattgaa ctcgaaatcg tcgctggttt cgttaaataa
    36481 catgatcttg gtgttttcca ggtgctgcca cattgccagc ttagcagcat gaggatcttt
    36541 gcgaatcagc gccttgagga tttgatcgtg gtcatcacac cagttatcga cggtacgggc
    36601 atcaatgtgt tcgtgcagtt ttttccagta cgggttatga ctacgctggg tccacatttt
    36661 ttcaacgata gccgccaggg cggagttctg cgtcgccagg gctacctgaa tatggaactg
    36721 caaatcccac tcggaatcac ggaagcattg ttcgccgcgc gcctgctcct ggatggccat
    36781 caatttcatg atgtcctgtt ttgtgacctg agttgccgcg aactcggcaa tattactttc
    36841 gatgagctgg cgggcttgca gtagctcaaa cggaccgtaa ttggcaaact ccatattatt
    36901 gtcagccgat tgctgatggc gcggctggtt ggaaaccaca tgaatacctg aacctttacg
    36961 cacttcgaca tagccttcaa cttccagcat gatgatggct tcacgaacca cggtgcggct
    37021 gacgtttttt tcatcggcaa taaagcgttc ggcaggcagt ttatcaccta ccagatagac
    37081 gccctgttcg atgcgctctt tcagatcagc ggcaagttgt tgatacaaac ggcgtggttc
    37141 agtgatttcc atatgcgtct ccaggagtgg aatgcggaca aagttatcat accactttat
    37201 gcatgcgtat gccattgtta aaatataaat cgaatgattg aaataatatt atttattgat
    37261 gttatgaata ttaatatatt cctaaaattg attatgttga attgtgaaaa aacatatttt
    37321 tcagtaggtt gtattttttt gagaataaat aattttatct aaagatgctt ttttgtgttg
    37381 acttgaaaaa tagtatgatt tctaatgcgc caacttttta tgaaataatt taactgatta
    37441 aatggtggcg aaaattaatc gattattacg gctcgggcct gtcttacttc tgtgcacatc
    37501 atttttcgca caatctaata tgactgtata tcctatgtct gtatctataa acagtcaggg
    37561 agaaggaagt gttcgtgtaa tatcgaaaac gaatgaagtc caatatataa aggcaaccgt
    37621 atttcgtatc gataatccat caacaccaca agaaaaagaa atcgaagtaa cttcaggcga
    37681 tgcaaaccaa cttgtgataa tgccgcccaa atttgctttg cctggcggta gtagcaaaac
    37741 cgtgcgtttt gtggcaatgg aacctgagca aaaagaaaaa aactatcggg ttaaatttga
    37801 agctgtaccc agcattgatg atgcaacaga taataaaaaa gatctctcca tgcaattaac
    37861 ggttaactta atttggggga ttgttgtcag cgtgccacct cagcagcctg tggcaaagtt
    37921 agatattaac gccgataaaa agctaatcaa taatggcaat caacgtctga agatattaac
    37981 cattgcctat tgtaaaaata gtggttctgc ggagtgcaga acacagaccg taaataaaaa
    38041 cattttccct ggtcaggaaa gagttttaga aagcatatct ggctacgata aaatcgttgt
    38101 caaatataat aactggatca ccaaggacaa tggcgagttt gagctggcgg tccattaatt
    38161 atcattgtaa taaaccaagg aatagtaatg aaaaaggtat tcgcaaaatc tcttctggta
    38221 gcggcaatgt tttccgttgc aggttctgca ttagcagtac aaaaagatat cactgttact
    38281 gctaacgttg atgctgctct ggatatgacg cagaccgata atactgcact gccgaaggca
    38341 gtagaaatgc aatatctgcc aggtcagggt ctgcaatctt accagttaat gactaaaatc
    38401 tggtcaaatg acacgaccaa agacgtgaaa atgcagttag tttcgcctgc gcaactggtt
    38461 cagagcctgg acgccagcaa aattgttccg ctgaccgtaa cctggggtgg tgaagagatt
    38521 aaagctgatg cggcaaccac ttttaccgca acgaaaatct ttgcttccga tgcgttaact
    38581 aacggttcgc tggctaaacc actgatgttt tcccaggcta ccaaaggtgt tctggaaacg
    38641 ggtatctacc gtggcgtagt tagcatttat ctgtcccagg ctctctaatc tggataacag
    38701 aattaacatg caataaaggg aggcaagtcc tcccttcttt taactatttt agtatttatt
    38761 atggattaca ggacagccat ggacaaaaaa ttactggctc ttttgatcct ggcgagtctc
    38821 agcccggcaa aggcaacatt aacaaaaatt cccgctggtt ttgaggttat tgctcaggga
    38881 cagcaggagt atatcgaggt ttattttgca gggaaaagtc tcggtaaata ttatgcgatg
    38941 gttaatcttg ataccgtaac ctttcttgat tcatcaagtt tatacagcaa acttgaattg
    39001 agcactgacg atcaaaaaat cgctcataca gtgaaagaaa aactatcgca acccttagct
    39061 cgccacggtg aactggcttg tggttttgta cgtactgatt caggatgcgg ttttcttaat
    39121 actgagacag tagaaataat ctacaatgat gaagaaagtt cagcaacgct atttcttaat
    39181 ccgcaatgga gttctgcgtt taactcgaag tcattgtatt taaatccaga caaaaatacg
    39241 gttaacgctt ttatacatca gcaagatatt aatgtgctgg tacaggatga ttaccaatcg
    39301 ttgtcaattc agggtaatgg cgcactggga ataacagaaa atagctatat tggtgcgcac
    39361 tggaatttcg acggttatga tgcagatgat gtcagcgaca acaatgtcga tgtcagcgat
    39421 ctctattacc gctatgattt tttacgccgt tattatgtgc aggcggggcg gatggacaac
    39481 cgcacattat ttaatgcgca aggtggaaac tttactttta actttctgcc actcggtgcc
    39541 atcgacggaa tgcgtatcgg gtcgactctc agttatttaa accagacgca aagccagcag
    39601 ggaacgccgg taatgatcct gctttcgcgc aattctcgtg ttgatgctta tcgtaatgaa
    39661 caactgttgg gatcgtttta cctcaatagt ggttcgcaat ttattgatac cagttccttt
    39721 ccgccaggca gctacagcgt ggcgttaaaa gtttatgaaa ataaccaact cacccgcacc
    39781 gagcttgtac cgtttaccaa aaccggcggt ctgacagacg gaaatgccca gtggttcttg
    39841 caggcaggta aaaccacatc acaggtttct gatgatgaaa gttctgctta tcagctaggc
    39901 gtacgcctgc cattacatcc gcaatatgag ctctacgcag ggctggcgaa tgccgatgat
    39961 gtaagtgctt tcgagttagg caataactgg acggcagatt taggcggggc ggggaatctg
    40021 gctatcagtg ccagcgtgtt ccgtaacgat gacggcggca aaggcgatat gcaacaggcc
    40081 aactggagtc atccgggatg gccgacgttg ggcttttatc ggaccaactc tgacggtgat
    40141 gcttgtacaa ccgacaacag agagagctac aacgccttaa gctgttatga aagtatttcc
    40201 gcgacggttt cacagaattt tgtcggctgg aatatgatgc tgggttatac ccgcacacaa
    40261 aataacactg atgatagttt gcgctgggac aaacagcaga gctttgaaaa taactatctt
    40321 cgccagactt cggctcaaag catttccgag accgtacaac ttagcgcttc tcgcgctttt
    40381 gtgatgcgtg actggatttt gagtacttcc cttggggttt tccatcgtaa tgacaacggc
    40441 ggtggtagcg atgacaacgg tttgtatctg tcgttttcgt tatctgatac gccgacgatg
    40501 gacagcaata acaacagcca ttcaaccaat gtttctactg attatcgtta tagcgatcag
    40561 gatggcgatc aaacgtcatg gcagttatcg catacctttt ataacgactc attcagccat
    40621 aaagagcttg gcgtgaccgt cggcggtttg aacaccgata ccataaacag cgcggttaac
    40681 gggcgttggg atggccaata tggaaatgtc tacgctactg tctcagacag ttatgatcgc
    40741 cagaaccatg atcatctctc ggccttcacc gggacataca gctccacgct ggcggtgagt
    40801 cgctatggca tcaatttggg ggccagcggc tcagatgatt tgctgggagc ggtgttggtg
    40861 gatgtgaaag gtttctctga acaggatgaa cagagtcagg gtctgcaact cgaagcgcgg
    40921 gtggctggca gcagaacatt gcagcttggt caaagcgaca gcgtgttatt cccctatcct
    40981 ggatttcagt ctggctttgt tgaagttaac gacagtaatc agggtaacca gcaaggaaca
    41041 accaacatca ttaacggtgc gggaaatcgt gagttaatgc tgttgccggg caaattacgt
    41101 tatcgcgaag tgtctgccag ttttaattac aactatattg gtcgtttgtt attgcccgca
    41161 tcggtagaga aattcccgct ggtgggtctg aatagcgcca tgttactggt ggctgaagat
    41221 ggcggattca cacttgagat cagcagtggt gaaaaagagt tgtatctgct ttccgggcag
    41281 cagttcctga aatgtccgct gaatgttttg aagaaacgcg ccagcattcg ctatagcggg
    41341 gatgtgaatt gtagcgtggt gagttattca caattgccgg aatctattca ggttcaggca
    41401 cagttgaaac agcctaaatt acgtggaaac gtccagacgg cgcaaaggga ggttgcacca
    41461 tgagaaatcg attaattgcc gtgattttat gtttatttgg catggtcgcg ggggggcacg
    41521 ctacgccaaa tgtgacggct gaaattactt atgatttggc cgctggtagg gcggattatt
    41581 acttctggaa tagggaagat ccccccgcag taagctacaa cacgacatgg tcaagttata
    41641 aatgcgattt tcctgatgtg cagcagacct gtaccgcatc aggaaattta tcaacagtga
    41701 aaatatattt gacagaaaaa cgcagtggaa tgcgttggcc cgtcaaactc aaaggctatg
    41761 ttgatgcgga ggtctggaac ccgggtggag tctgtaacgg atggtcgacg caaatcgcgt
    41821 tagctaacgg tacgggttat caatgtaaaa gtgcttcaga cggttatata caacatcttg
    41881 cgagcgcaaa gccgatgacg ctctatcttg aacagtccga aatgaaaaat ttaccaatcg
    41941 gtggtgtttg ggaaggttcg gtcaaattgc agtttactaa tccgtctacg gattatcgcg
    42001 ccgatattac gcttaatgtc ctcgatccca accatatcga cgtgttcttc ccggagttcg
    42061 cccacgctac gccacgcgta caactggatt tgcatccaac aggcagcgtt aacggcaaca
    42121 actacgcgca agatctgact atgttggata tgtgcctgta cgatggcttt aacggtaacg
    42181 gtcttagcta tgaaattttg ctaaaagatg agggaagaac ggcggcagga cgcagtaatg
    42241 gtgagttttc gatttatcgt cagggcgcga gctcaacgga tgaaggggag cgtattgatt
    42301 accgtgtcaa aatgtacgac ccggaatcag gtgggcaaat cgatgtgcgc aacaatgaga
    42361 gcatggtctg gaccaacatc aaccttaaac gtgtccgccc ggtggtgctt ccaggcattc
    42421 gttacgctgt gatgtgtgtt cccacaccgc tgacgctggt ggtcgacaaa tttaacgtga
    42481 cggcaaaaca ggcgggatat tatatgggta aattgtcggt catctttacc ccgtcattgc
    42541 cgacaatcaa ttgacctcgc agaccagaaa aaactaaaca acgaagccgc ctgaagaggc
    42601 ggcttcgtca ttacaactta cgttaattct taatgttgcg gtgcgggatc gttatgtgat
    42661 tcctgcgcca cctcaatcgc cggtttgttc tgcaataccg tccagataac cagtgccccc
    42721 agtaggtcga ataccgcaag tactgcgaac agcgggctaa agccgatggt gtcagccagt
    42781 gcaccaacca ccagagcaaa gagcgtactt gccagccatg cggacatccc ggttaaaccg
    42841 ttcgccgtcg ccacttcgtt acgaccgaac acgtcagaag agagcgtaat cagtgcgcca
    42901 gacagtgcct ggtgagcaaa accaccgata cacagcagca taatcgcgac atacgggttg
    42961 gtgaacagac cgatcatacc cgggccaatc atcagcacag cacccagcgt tacgaccatc
    43021 ttacgggaaa cgatcaggtt cacaccaaac caacgctgga acagcggcgg caggtaacca
    43081 ccgaggatac aaccgaggtc agcaaacagc atcggcatcc aggcaaacat cgcgatctct
    43141 ttcaggttaa agccgtaaac tttaaacatg aacagcggga tccacgcgtt aaaagtaccc
    43201 catgccggtt ctgccaggaa gcgcggcagc gcgatacccc agaactgacg gttgcgcagg
    43261 atctgaccaa cggacatttt cttcgctgtg tcaacctggt gctgggcttc ctgaccatta
    43321 ataatgtagt cgcgttcttc atcggtcaga tgtttctggt cgcgcggatg cttgtagaaa
    43381 atcagccatg ccatcgccca gataaagctc aacgcaccgg agatgataaa tgccatctgc
    43441 cagctgtgca tcacgattgc ccataccacc agcggcggcg caatcatcgc accaatcgaa
    43501 gaacctacgt taaagtaacc taccgcgatg gaacgctctt tcgccgggaa ccattcggag
    43561 ctggctttca gacccgccgg aatcatcgcg gcttccgcgg caccgaccgc accacgagca
    43621 accgccaggc caccccaact acctgccagc gcggttgcac cacagaacac ggcccacagt
    43681 acagcaaaca ttgcataacc gattttcgta cccagcacat ccagtacata acccgctacc
    43741 ggttgcatga ccgtataagc agcagaatac gctgcgatga tataggaata ctgttgggtg
    43801 gagatgttta actcttccat cagagttggc gcagctgccg ccacagtgtt acgcgtcagg
    43861 taaccaagca cggtgccgag cgtcaccagt gcgatcatat accaacgtaa ccctttaatt
    43921 ttacgcatgt aaaacctcat cgttttgtta tatccgcagg aagcgcggcc atcatcccgc
    43981 cagaggctga agatgttagt tacgaccggg taaccgaaaa aaaaccggca cgccccgaac
    44041 gttgccatga aagtttcgtg ctaattcggc ttccgtaccg gttaacccga ttcatctaac
    44101 aaatgaatga atcggcaatt tgcattccgt cgacttatga tttgcgacgg cagaaagata
    44161 acttgtcata caactttaaa aggtgagagc catcacaaat gtgcgaatat ttgtagggac
    44221 attacctgac gaccacgagg ccagtactgg cgcggcctgc agcgagattt accactttga
    44281 gagtaatttt tttaactacg tttattgatc taactcacga aaatatcttc ggactctgga
    44341 aattggtgtg ataactttgt cagcatcgca ccataagcaa gctagctcac tcgttgagag
    44401 gaagacgaaa atgactccgt ttatgactga agatttcctg ttagataccg aatttgcccg
    44461 ccgtctgtat cacgactacg caaaagacca gccgattttc gattaccatt gccatttgcc
    44521 gccgcagcag attgcggaag actatcgttt taaaaacctg tatgacatct ggttgaaggg
    44581 cgatcactac aaatggcgcg ctatgcgtac caacggtgtg gccgagcgtc tgtgcaccgg
    44641 tgatgcgtct gaccgtgaaa aatttgacgc ctgggcggcg actgttccgc atactatcgg
    44701 caacccgtta taccactgga cgcacctcga actgcgtcgt ccgtttggta tcactggcaa
    44761 attgctttct ccgtcaactg ccgatgaaat ctggaacgaa tgtaacgaat tgctggcgca
    44821 ggataatttc tctgcacgcg gcatcatgca gcagatgaac gtgaaaatgg tcggcaccac
    44881 cgatgacccg atcgattctc tggagcatca cgcagagatc gccaaagatg gctctttcac
    44941 cattaaagtg ctgccgagct ggcgtccgga caaagccttt aacattgaac aggcgacctt
    45001 taacgactac atggcgaagc tgggcgaagt ttccgatacc gacattcgcc gctttgctga
    45061 cctgcaaaca gccctgacta aacgtctgga tcacttcgcc gctcacggct gtaaagtgtc
    45121 tgaccacgcg ctggatgtag tgatgtttgc tgaagcgaac gaagcggaac tggacagcat
    45181 cctcgcgcgc cgtctggcag gcgaaaccct gagcgagcac gaagtggcac agttcaaaac
    45241 tgcggtgctg gtgttcctcg gcgctgaata cgcacgtcgc ggctgggtac agcagtacca
    45301 tattggcgca ctgcgtaata acaacctgcg tcagtttaaa ctgctggggc cggatgtcgg
    45361 ctttgactcc atcaacgacc gtccgatggc ggaagagctg tctaagctgc tgagcaagca
    45421 gaacgaagaa aacctgctgc cgaaaaccat tctctactgc ctgaacccgc gcgataacga
    45481 agtgctgggc accatgatcg gtaacttcca gggcgaaggt atgccgggca aaatgcagtt
    45541 cggttccggc tggtggttta acgaccagaa agacggtatg gaacgtcaga tgacccaact
    45601 ggcgcagctc ggtctgctga gccgctttgt cggtatgctg actgacagcc gtagcttcct
    45661 gtcatacacc cgtcacgaat acttccgccg cattctgtgc cagatgatcg gtcgctgggt
    45721 ggaagcaggc gaagcaccgg cggacatcaa cctgctgggc gagatggtga aaaatatttg
    45781 ctttaacaat gcgcgtgact acttcgccat tgaactgaac taaggtctgg ggttgatatg
    45841 caatacatca agatccatgc gctggataac gtcgcggttg ctttagcgga tttggctgaa
    45901 ggcacagaag tcagtgtcga taaccagact gttacgctgc gccaggatgt tgctcgtgga
    45961 cataaatttg cgttaacgga tatcgcaaaa ggggccaacg tcattaaata tggcctgccg
    46021 attggttatg cattggcgga tattgctgcg ggggaacacg ttcacgccca caatacgcgc
    46081 acgaatctga gcgatctgga tcagtatcgc tatcaacctg attttcagga tctgcctgcg
    46141 caagcggcag atcgtgaagt gcagatctat cgtcgcgcga acggcgatgt cggggtgcgt
    46201 aatgagctgt ggatcctgcc aaccgtgggt tgtgttaacg gcatcgcgcg gcagatccag
    46261 aaccgtttcc tgaaagagac caacaacgct gaaggtaccg acggcgtgtt cctcttcagc
    46321 cacacctacg gctgctcaca actgggcgac gatcacatca atactcgcac catgctgcaa
    46381 aacatggtgc gccacccgaa cgcgggcgca gtgctggtga ttggtctggg ctgtgaaaac
    46441 aaccaggttg ccgcattccg tgaaacgttg ggcgatatcg atcctgaacg cgttcatttc
    46501 atgatctgcc aacagcagga tgatgaaatc gaggccggga tcgcgcattt gcatcagctg
    46561 tataacgtga tgcgcaacga taaacgcgag ccaggcaaac tcagcgaact gaagtttggt
    46621 ctggagtgcg gtggttctga cggtctttct ggtattactg ctaacccgat gctggggcgt
    46681 ttctctgact acgtgattgc taacggtggt actaccgtac tgaccgaagt gccggagatg
    46741 tttggcgcag agcagttgct gatggaccat tgccgcgacg aagcaacgtt tgaaaaactg
    46801 gtcaccatgg tcaacgactt caaacagtac tttattgccc atgaccagcc gatctacgaa
    46861 aacccatcac cggggaacaa agcgggcggt atcaccacgc tggaagacaa atcacttggc
    46921 tgtacccaga aagcgggttc cagtgtcgtg gttgacgtgc tgcgttacgg cgagcgtctg
    46981 aaaacgccgg ggctgaactt gttaagtgcg ccgggtaacg atgccgtagc gaccagcgcc
    47041 ctggcgggtg cgggctgcca tatggtgctg ttcagtactg gtcgtggtac gccgtatggt
    47101 ggatttgtgc cgacggtgaa aatcgccacc aacagtgaac tggcggcgaa gaaaaaacac
    47161 tggatcgact ttgacgcggg tcagctgatc cacggtaaag cgatgccgca gttgctggaa
    47221 gaatttatcg ataccatcgt tgagtttgcc aacggtaagc aaacctgcaa cgagcgtaac
    47281 gacttccgtg aactggcgat ctttaaaagc ggcgtaacgc tataaagtcg aaatgagttg
    47341 ttaataaaaa acggcgtttc ataatgtgaa gcgccgtttg cttttccata cctgctaagg
    47401 atttatcatg accgcgtatt ggctggccca gggcgtgggt gtcatcgcct ttctgattgg
    47461 tatcacaaca tttttcaatc gtgacgaacg tcgcttcaaa aagcagcttt cggtctatag
    47521 cgccgttatt ggcgtacatt tttttcttct gggcacctat cctgccggtg ccagtgccat
    47581 ccttaatgcc attcgtacat tgattacctt acgcacgcgc agcttatggg taatggcgat
    47641 ttttattgtg ctgactggcg gaattggcct cgcgaagttc catcatcctg tcgaactact
    47701 gccggtaatc ggcacgattg tcagtacctg ggcgctgttc cgctgtaaag ggctgaccat
    47761 gcgctgcgta atgtggtttt caacgtgttg ctgggtgatt cacaacttct gggcggggtc
    47821 gataggcggc acgatgattg aggggagttt tctgctcatg aatggcctga atatcattcg
    47881 tttctggcgg atgcagaaaa ggggaattga tccgtttaaa gtggagaaaa ccccttccgc
    47941 cgtagacgaa aggggctaag tattagttac gcagggcgct atttgccaga cgatcgtctt
    48001 ccgcctggca agctgccgca gtgaacagca cgtcagttga agagttcagc gcggtttcgc
    48061 aagagtcctg caatacgccg atgataaagc cgacggcaac cacctgcatg gcgatatcgt
    48121 tcgaaatacc gaacatatta caggccagtg ggatcagcag cagagacccc cccgccacgc
    48181 cggatgcgcc acaggcacac agagaagcca ctacgcttaa cagcagcgct gtgggcagat
    48241 ccaccggaat acccagcgta ttaaccgcag ccagcgtcaa cacggtaatg gtgattgctg
    48301 cgcccgccat attgatggtg gctcccagcg gaatagaaac ggaataggta tcgcgatcca
    48361 gattcagctt ttcacacagc gccatattca ccggaatgtt cgctgcagag ctgcgggtga
    48421 agaaggcata cacgccgctt tcgcgcaggc acagcagcac cagcgggaac gggttacgac
    48481 gaatcttcca ccacaccagc aaggggttaa ccaccagcgc caccagcagc atacagccaa
    48541 ccagcacgac cagcagttgc gcgtagcccc acagtgtgga gaaaccggtg gttgccaggg
    48601 tagaagaaac cagaccaaaa ataccgatcg gtgcgaagcg aatgaccagt ttcaccataa
    48661 aggtgacggc attcgacata tcgttgacca ggtttttggt ggtctcgtta ccgtgacgca
    48721 gtgcgaaacc gaggccaatt gcccacacca ggatcccgat atagttacct ttcagcagcg
    48781 cgtcaatggg gttggaaacc atgctcatta ccagcccgcg cattacttct acaatgcctg
    48841 acggcggcga aatatcaccc gcgctactgg acaagtgcag ggtagaaggg aaggcaaaac
    48901 tgaagattac tgcggccaga gcagcagaga aggtgcccag cagatagagg aacaaaatag
    48961 ggcggatatt ggttttctgc ccgtgctggt ggttagcaat agatgccatt accagcatca
    49021 acaccaggat gggggcaacg gctttcagtg cgccgacgaa caaagtacct aacagaccaa
    49081 cagcttccgc cgcgggtttt gagatccatg ccagaagaat ccccagaaca aggccgacca
    49141 ggatttgttt taccaggctg ccatgagcca gacgccggaa tagccccggt gaacgttgcg
    49201 tagtcatttt tcgatccttt cattgtgttg tcgcacatcc ctggtgcgtt ctgtcgacgc
    49261 atctttcagg atgtaacaaa gtgtttgcaa ggtcgagtat aaggaaagtt acagcttcgg
    49321 gaagcgaaaa atgctggatt ttaaagagag gtcatatttt gcaacttaat ggttacattt
    49381 gtttgacata aaaataaagc ccaacgtgca gcttccagac tgatgacaac gtcaaaaaag
    49441 tactcggtca gtcccctcgc cccttttggg gagagggaga aaacggtgct catgttgatg
    49501 acagggtaca cggcgtgggc agattaaaaa tcatccaacc cgctgcttat catgccgata
    49561 attcacccag gcgttgataa taaacgtcat caccagaatg ccaaacacca cgcccagcga
    49621 gacggcgatc ggaatatggt agaagtcgac aatcagcatc ttgataccga taaacaccag
    49681 aatcaccgcc agaccatatt tgagcatcga gaaacgctct gccacacctg ccagcaggaa
    49741 atacatcgca cgcaggccga ggatcgcaaa caggtttgag gtcagcacaa taaacggatc
    49801 ggtggtcacg gcgaagatag ccggaatact atccacggcg aaaatcacgt cgctcaactc
    49861 caccagaatc agcaccagca tcagcggcgt ggcatacaac aaaccattct tacgcacaaa
    49921 gaaatgctcg ttgtcgatgg tgtcggtcat gcgtaaatga ccgcgtagcc agcgcaccag
    49981 cggcttgtcg ccaatgcctg attcatcttc atgggcgagc gccatcttca cgccggtaaa
    50041 cagcaggaag gcaccgaaga tatacaagat ccagtcgaac tgtgaaatca gccagctacc
    50101 agtgaagatc atgatggtac gcagaacaat cgccccgagc acgccataca ccagcacgcg
    50161 acgttgtaat gccgccggaa cagagaaata gctgaacaac atcagccaga caaagacgtt
    50221 atcgaccgcc agcgatttct caatcagata accggtgagg aaggccagtg cctgtggatc
    50281 ggcaacggcg cgaccttcgg tttgcaccag ataccaccag aaagcggcgt taaacagtaa
    50341 cgacagcgtc acccagacca gcgaccaggc cgcagcctgt ttcatggtca tggcatgtgc
    50401 cccacgacgc ccctgcaaca acaggtcgat agccagcata atggtgacaa caacagcgaa
    50461 tccgccccat agcaacggcg tgccgacagt attcatagaa gttccttaca cataaaaaaa
    50521 cggccaacgt cggaagacgt cagccgctgc tttttatgca tagacctcgc cttccggcaa
    50581 ggtctcactt acaacaaaaa aggaatacgt ctgcgtattc ctttcgggtt gcccggtgac
    50641 cggatgtggt ttttcacaca tcgtaatgac gatcgaccgg caatgaagtt actccccttt
    50701 gcaggtaaca aagtatgaca gaccattcag tcagtcaatg gtctgttaca taccttttac
    50761 actgttttac tttggcaatt tatagtttta cgctatctgc cggaaaaatc accctagtct
    50821 ggcggcggat ctcggtcagc agtttggcgg tgatgcgact gaccgccagc cctggatgat
    50881 ccaccagatg ctcatccacc agggtagcga acagctctgc ttcgtagagc atggtattaa
    50941 tatgctgcgg ttgggtgaga tcctgcatct ggctgccacg cggaacaaag caaactttct
    51001 ggcattcaga cagtttttca atcaccagcg atcccgcttc gccctgaatc tcgcttgcca
    51061 ggacagaatc gctcactttt gagtgctgca acgtaacgct gaaatcaccg taatccatca
    51121 ccaccacgcc gtgggcatcc acaccgcttg ccagcaaact ggcggttgcc tgcacgcttt
    51181 tcggttcacc gaataatgcc accgccgacg ccaggcagta aaagccgata tccataattg
    51241 aaccgttaga gaatgccgga ttaaaggtgt tgggattctc accatccagg taacgttgat
    51301 accgcgagga atactggcaa tagttgaaaa agactttacg cagtttgccg actttcggca
    51361 gcgcctggcg caacagatga aagttcggca ggctggcggt tttaaatgcc tcaaacagca
    51421 ccacctgatt ttcccgcgca caggcaatgg cggcatccac ttccgccaga ttcgacgcca
    51481 gtggcttctc gcaaatcaca tgaattttgt gactaaggaa aagttgtgtc tgggaaaagt
    51541 gcagggaatt cgggctggca atatacaccg cgtcaatggc atcgctttct gccatcgctt
    51601 ccagcgacgt aaacagatgc tcgacagaaa aatcattggc gaagtgctgg gcctgttcaa
    51661 gactgcggga ataaacggcg gttaacttgt atttaccgct ctcatgggcg gcctcgacga
    51721 actggcgagt gatccagttc gtaccaatca cagcgaaacg tatcataacg cgtggatact
    51781 ccataaaggg attctctcgt cactgtagca cgcctttttt atcttcgacg tgcgcagata
    51841 cgcattattc ttttgccgga cagccatgct cactaaacgg cccaatcata accaacggcc
    51901 cgagatattt cggctggcga tggaggattt tcgcatcaat caccgcgctg actctggcaa
    51961 gtttttcccg cagttgagta tataaaccgt gtttcatatt caggtctggc gcgttaaaac
    52021 cgatagcatc gataccatac tgttttgcca gccagatggc gcgttggtta tgaaattctt
    52081 gcgagataat ggtgatatgg ttttcactaa acaccttgtt ggcacgcacc accgaatcaa
    52141 gcgttgagaa tccggcatag tcgcagaaga tcacttttgc cggtacgcct ttagcgatca
    52201 aggcttgctg cattcctgac gcttcatcgt aatttttacg tccattatcg ccgctcacca
    52261 atagccattt cacttttccg gcgtggtaca actctgcggc gctatcaata cgtcgggtaa
    52321 agtagcgatt acccggcctg gctcccagca ataaacccac gttccgcgcc ggaacggcgt
    52381 tgacatcgct ccaggtcagt tgttggctgg cgtttaccat aacgcgatcg gcaataaaaa
    52441 tggtcgctcc tgctataagc aacaataaac agccaatctt aagagtccgg cgagaaaaac
    52501 aaatacggag aagaaggcgg ggctgcatgg tcgttatcct taacgaaagg ggggaggtcg
    52561 attttaggcg ctataggaag aaaaaataac gcctgacatc attttcagat tttgtcctgc
    52621 ccaagcgaca actgcgttag ccacagccgg gtgtcgaact caagctggtg gtactgcggc
    52681 tccatatggc agcacaattg atagaacgct ttgttatgct ctttctcctt caggtgcgcc
    52741 aactcgtgca ccacgatcat gcgcaaaaac ggttccggcg cgttgcgaaa cacggtagcg
    52801 acgcggatct ccgcctttgc tttcagcttg ccgccctgca cacgcgaaac agcggtatgt
    52861 aacccgagcg catttttcag cacatggatc ttgttgtcat acatcacttt attgatcggc
    52921 ggggcattgc gcagaaactg atttttaaga tcctgagtat attgccagag ggctttatcg
    52981 gtggcgtagt cgtgtgttcc cggatagcgt tttgccagca catcgcccag acgctgttcg
    53041 ttaatcagcg ttcgcacctg ggaaagtagc tgctcgggat aaccctgaag ataagtaaga
    53101 ttgctcataa ccgcccacga aaaagagaaa aagggtatac tcacgcaccc ttttcagggg
    53161 caacgtcgaa attttatcat tcaggagggc cgatgagcca cttagacaac ggtttccgtt
    53221 cactgacact acaacgtttt ccggcgacgg atgacgttaa cccgctacag gcgtgggaag
    53281 cggcggatga atatttgctg caacagttgg acgacacaga aatccgcggc ccggtgttga
    53341 tcctgaatga tgcctttggt gcgttaagtt gtgcgctggc ggaacataag ccgtacagca
    53401 ttggcgactc atacatcagt gaactggcca cgcgcgagaa tttacgcctc aacgggattg
    53461 atgaatcgag cgtgaagttt ctcgacagca ccgccgacta cccgcaacag ccgggcgtgg
    53521 tactgatcaa agtaccgaaa acactggcgt tgctggaaca acaactgcgt gcgctgcgca
    53581 aagtggtcac gccggataca cgtattattg ccggtgctaa agcccgtgac attcacacct
    53641 ccacgctgga actgttcgaa aaagtgctcg gtccgaccac caccacactg gcatggaaga
    53701 aagcgcgcct gattaactgc actttcaacg agccgccgct ggttgatgca ccgcagaccg
    53761 ttagctggaa gctggaaggt actgactgga ctatccacaa ccatgcgaat gtcttctccc
    53821 gcaccgggct ggatattggc gcgcgcttct ttatgcagca tctgccagag aatctcgaag
    53881 gtgagattgt cgatctcggt tgtggtaatg gcgttattgg tctgacgctg cttgataaaa
    53941 acccgcaggc gaaagtggtg tttgtcgatg aatcgccgat ggcggttgct tccagccgtt
    54001 tgaacgttga aaccaacatg ccagaggcgt tggatcgctg cgagtttatg attaacaacg
    54061 cgctctccgg cgtggagcct ttccgcttta atgctgtgct ctgcaacccg ccgtttcatc
    54121 agcaacatgc gctgaccgat aacgtcgcct gggagatgtt ccaccacgcc cgccgctgtc
    54181 tgaaaatcaa cggcgagctg tatatcgttg
//
LOCUS       DAMZFT010000016        51542 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.016, whole genome shotgun sequence.
ACCESSION   DAMZFT010000016 DAMZFT010000000
VERSION     DAMZFT010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 51542)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 51542)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..51542
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.016"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            91..165
                     /locus_tag="PN977_001100"
     tRNA            91..165
                     /locus_tag="PN977_001100"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:123..125,aa:Gln,seq:ctg)
     gene            complement(381..1556)
                     /gene="ubiF"
                     /locus_tag="PN977_001101"
     CDS             complement(381..1556)
                     /gene="ubiF"
                     /locus_tag="PN977_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-3-methyl-6-methoxy-1,4-benzoquinol
                     hydroxylase"
                     /protein_id="HDI6041157.1"
                     /translation="MTNQPTEIAIVGGGMVGGALALGLAQHGFSVMVIEHAQPAPFVA
                     DSQPDVRISAISAASVSLLKGLGVWDAVQAMRCHPYRRLETWEWETAHVVFDAAELKL
                     PLLGYMVENTVLQQALWQALEAHPKVTLRVPGSLIALHRHNDLQELELKGGETIRAKL
                     VIGADGANSQVRQMAGIGVHAWQYAQSCMLISVQCENDPGDSTWQQFTPDGPRAFLPL
                     FDNWASLVWYDSPARIRQLQNMNMAQLQAEIAKHFPSRLGYVTPLAAGAFPLTRRHAL
                     QYVQPGLALVGDAAHTIHPLAGQGVNLGYRDVDALIDVLVNARSYGEAWASYPVLKRY
                     QMRRMADNFIMQSGMDLFYAGFSNNLPPLRFMRNLGLMAAERAGVLKRQALKYALGL"
     gene            1702..3126
                     /gene="miaB"
                     /locus_tag="PN977_001102"
     CDS             1702..3126
                     /gene="miaB"
                     /locus_tag="PN977_001102"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HDI6041158.1"
                     /translation="MTKKLHIKTWGCQMNEYDSSKMADLLDATHGYQLTDVAEEADVL
                     LLNTCSIREKAQEKVFHQLGRWKLLKEKNPDLIIGVGGCVASQEGEHIRQRAHYVDII
                     FGPQTLHRLPEMINSVRGDRSPVVDISFPEIEKFDRLPEPRAEGPTAFVSIMEGCNKY
                     CTYCVVPYTRGEEVSRPSDDILFEIAQLAAQGVREVNLLGQNVNAWRGENYDGTTGSF
                     ADLLRLVAAIDGIDRIRFTTSHPIEFTDDIIEVYRDTPELVSFLHLPVQSGSDRILNL
                     MGRTHTALEYKAIIRKLRAARPDIQISSDFIVGFPGETTEDFEKTMKLIADVNFDMSY
                     SFIFSARPGTPAADMVDDVPEEEKKQRLYILQERINQQAMAWSRRMLGTTQRILVEGT
                     SRKSIMELSGRTENNRVVNFEGTPDMIGKFVDVEITDVYPNSLRGKVVRTEDEMGLRV
                     AETPESVIARTRKENDLGVGYYQP"
     gene            3279..4319
                     /locus_tag="PN977_001103"
     CDS             3279..4319
                     /locus_tag="PN977_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415193.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HDI6041159.1"
                     /translation="MNIDTREITLEPADNARLLSLCGPFDDNIKQLERRLGIEINRRD
                     NHFKLTGRPICVTAAADILRSLYVDTAPMRGQIQDIEPEQIHLAIKEARVLEQSAESV
                     PEYGKAVNIKTKRGVIKPRTPNQAQYIANILDHDITFGVGPAGTGKTYLAVAAAVDAL
                     ERQEIRRILLTRPAVEAGEKLGFLPGDLSQKVDPYLRPLYDALFEMLGFEKVEKLIER
                     NVIEVAPLAYMRGRTLNDAFIILDESQNTTIEQMKMFLTRIGFNSKAVITGDVTQIDL
                     PRNTKSGLRHAIEVLADVEEISFNFFHSEDVVRHPVVARIVNAYEAWEEAEQKRKAAL
                     AAERKREEQEQK"
     gene            4316..4783
                     /gene="ybeY"
                     /locus_tag="PN977_001104"
     CDS             4316..4783
                     /gene="ybeY"
                     /locus_tag="PN977_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014169133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HDI6041160.1"
                     /translation="MSQVILDLQLACEDNSGLPEESQFQTWLNAVIPQFQEESEVTIR
                     VVDTAESHSLNLTYRGKDKPTNVLSFPFEVPPGMEMSLLGDLVICRQVVEKEAQEQGK
                     PLEAHWAHMVVHGSLHLLGYDHIEDDEAEEMEAIETEIMLALGYEDPYIAEKE"
     gene            4873..5751
                     /gene="corC"
                     /locus_tag="PN977_001105"
     CDS             4873..5751
                     /gene="corC"
                     /locus_tag="PN977_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308723.1"
                     /note="CorC(YbeX) belongs to the Cyclin M Mg2+ Exporter
                     (CNNM) family, and was characterized as belonging to a set
                     of three proteins, at least one of which must be present
                     for CorA to function; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family magnesium/cobalt transport protein
                     CorC"
                     /protein_id="HDI6041161.1"
                     /translation="MSDDNSHSSDTISNKKGFFSLLLSQLFHGEPKNRDELLALIRDS
                     GQNDLIDEDTRDMLEGVMDIADQRVRDIMIPRSQMITLKRNQTLDECLDVIIESAHSR
                     FPVISEDKDHIEGILMAKDLLPFMRSDAEAFSMDKVLRQAVVVPESKRVDRMLKEFRS
                     QRYHMAIVIDEFGGVSGLVTIEDILELIVGEIEDEYDEEDDIDFRQLSRHTWTVRALA
                     SIEDFNEAFGTHFSDEEVDTIGGLVMQAFGHLPARGETIDIDGYQFKVAMADSRRIIQ
                     VHVKIPDDSPQPKLDE"
     gene            5776..7314
                     /gene="lnt"
                     /locus_tag="PN977_001106"
     CDS             5776..7314
                     /gene="lnt"
                     /locus_tag="PN977_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HDI6041162.1"
                     /translation="MAFASLIERQRIRLLLALLFGACGTLAFSPYDVWPAAIISLMGL
                     QALTFNRRPLQSAAIGFCWGFGLFGSGINWVYVSIATFGGMPGPVNIFLVVLLAAYLS
                     LYTGLFAGVLSRLWPKTTWLRVAIAAPALWQVTEFLRGWVLTGFPWLQFGYSQIDGPL
                     KGLAPLMGVEAINFLLMMVSGLLALALVKRNWRPLVVAVVLFALPFPLRYIQWFTPQP
                     EKTIQVSMVQGDIPQSLKWDGDQLLNTLKIYYNATAPLMGKSSLIIWPESAITDLEIN
                     QQPFLKALDGELRDKGSSLVTGIVDARLNKQNRYDTYNTIITLGKGAPYSYESADRYN
                     KNHLVPFGEFVPLESILRPLAPFFDLPMSSFSRGPYIQPPLSVNGIELTAAICYEIIL
                     GEQVRDNFRPDTDYLLTISNDAWFGKSIGPWQHFQMARMRALELARPLLRSTNNGITA
                     VIGPQGEIQAMIPQFTREVLTTNVTPTTGLTPYARTGNWPLWVLTALFGFAAVLMSLR
                     QRRK"
     gene            7711..8619
                     /gene="gltI"
                     /locus_tag="PN977_001107"
     CDS             7711..8619
                     /gene="gltI"
                     /locus_tag="PN977_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter
                     substrate-binding protein GltI"
                     /protein_id="HDI6041163.1"
                     /translation="MQLRKPATAILALALSAGLAQADDAAPAAGSTLDKIAKNGVIVV
                     GHRESSVPFSYYDNQQKVVGYSQDYSNAIVEAVKKKLNKPDLQVKLIPITSQNRIPLL
                     QNGTFDFECGSTTNNVERQKQAAFSDTIFVVGTRLLTKKGGDIKDFADLKDKAVVVTS
                     GTTSEVLLNKLNEEQKMNMRIISAKDHGDSFRTLESGRAVAFMMDDALLAGERAKAKK
                     PDNWEIVGKPQSQEAYGCMLRKDDPQFKKLMDDTIAQVQTSGEAEKWFDKWFKNPIPP
                     KNLNMNFELSDEMKALFKEPNDKALN"
     gene            8789..9529
                     /gene="gltJ"
                     /locus_tag="PN977_001108"
     CDS             8789..9529
                     /gene="gltJ"
                     /locus_tag="PN977_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltJ"
                     /protein_id="HDI6041164.1"
                     /translation="MSIDWNWGIFLQQAPFGNTTYLGWIWSGFQVTIALSICAWIIAF
                     LVGSFFGILRTVPNRFLSGLGTLYVELFRNVPLIVQFFTWYLVIPELLPEKIGMWFKA
                     ELDPNIQFFLSSMLCLGLFTAARVCEQVRAAIQSLPRGQKNAALAMGLTLPQAYRYVL
                     LPNAYRVIVPPMTSEMMNLVKNSAIASTIGLVDMAAQAGKLLDYSAHAWESFTAITLA
                     YVLINAVIMLVMTLVERKVRLPGNMGGK"
     gene            9529..10203
                     /gene="gltK"
                     /locus_tag="PN977_001109"
     CDS             9529..10203
                     /gene="gltK"
                     /locus_tag="PN977_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltK"
                     /protein_id="HDI6041165.1"
                     /translation="MYEFDWSSIVPSLPYLLDGLVITLKITVTAVVIGILWGTMLAVM
                     RLSSFAPVAWFAKAYVNVFRSIPLVMVLLWFYLIVPGFLQNVLGLSPKNDIRLISAMV
                     AFSMFEAAYYSEIIRAGIQSISRGQSSAALALGMNHWQSMKLIILPQAFRAMVPLLLT
                     QGIVLFQDTSLVYVLSLADFFRTASTIGERDGTQVEMILFAGFVYFVISLSASLLVSY
                     LKRRTA"
     gene            10203..10928
                     /gene="gltL"
                     /locus_tag="PN977_001110"
     CDS             10203..10928
                     /gene="gltL"
                     /locus_tag="PN977_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020245804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter ATP binding
                     protein GltL"
                     /protein_id="HDI6041166.1"
                     /translation="MITLKNVSKWYGHFQVLTDCSTEVKKGEVVVVCGPSGSGKSTLI
                     KTVNGLEPVQQGEITVDGIVVNDKKTDLAKLRSRVGMVFQHFELFPHLSIIENLTLAQ
                     VKVLKRNKAPAREKALKLLERVGLSAHANKFPAQLSGGQQQRVAIARALCMDPIAMLF
                     DEPTSALDPEMINEVLDVMVELANEGMTMMVVTHEMGFARKVANRVIFMDEGKIVEDS
                     PKDAFFDDPKSDRAKDFLAKILH"
     gene            11046..11981
                     /gene="rihA"
                     /locus_tag="PN977_001111"
     CDS             11046..11981
                     /gene="rihA"
                     /locus_tag="PN977_001111"
                     /EC_number="3.2.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-specific ribonucleoside hydrolase
                     RihA"
                     /protein_id="HDI6041167.1"
                     /translation="MALPILLDCDPGHDDAIAIVLALASPELDVKAITSSAGNQTPEK
                     TLRNVLCMLTLLNRTDIPVASGAVKPLMRDLIIADNVHGESGLDGPALPEPTFAPQNC
                     TAVELMAKTLCESEEPVTIVSTGPQTNVALLLNSHPELHSKIARIVIMGGAMGLGNWT
                     PAAEFNIYVDPEAAEIVFQSGIPVVMAGLDVTHKAQIHVEDTERFRAIGNPVSTIVAE
                     LLDFFLEYHKDEKWGFVGAPLHDPCTIAWLLKPELFTTVERWVGVETQGKYTQGMTVV
                     DYYYLTGNKPNATVMVDVDRQGFVDLLADRLKFYA"
     gene            12065..13735
                     /gene="hscC"
                     /locus_tag="PN977_001112"
     CDS             12065..13735
                     /gene="hscC"
                     /locus_tag="PN977_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HscC"
                     /protein_id="HDI6041168.1"
                     /translation="MDNAELAIGIDLGTTNSLIAVWKDGAAQLIPNKFGEYLTPSIIS
                     MDENNHILVGKPAVSRRTSHPDKTAALFKRAMGSNTNWRLGSDTFNAPELSSLVLRSL
                     KEDAEEFLQRPIKDVVISVPAYFSDEQRKHTRLAAELAGLNAVRLINEPTAAAMAYGL
                     HTQQNTRSLVFDLGGGTFDVTVLEYATPVIEVHASAGDNFLGGEDFTHMLVDEVLKRA
                     AVAKNMLNESELAALYTSVEAAKCSNQLPLQISWQYQEETRECEFYENELEDLWLPLL
                     NRLRVPIEQALHDARLKPSQIDSLVLVGGASQMPLVQRIAVRLFGKLPYQSYDPSTIV
                     ALGAAIQAACRLRSEDIEEVILTDICPYSLGVEVNRQGVSGIFSPIIERNTTVPVSRV
                     ETYSTMHPEQDSITINVYQGENHKVKNNILVESFDVPLKKTGAYQSIDIRFSYDINGL
                     LEVDVLLEDGSVKSRVINHSPVTLSTQQIEESRARLSALKIYPRDMLINRTFKAKLEE
                     LWARALGDEREEIGRVITDFDAALQSNDMARVDEVRRRASDYLAIEIP"
     gene            complement(13846..15297)
                     /gene="djlC"
                     /locus_tag="PN977_001113"
     CDS             complement(13846..15297)
                     /gene="djlC"
                     /locus_tag="PN977_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlC"
                     /protein_id="HDI6041169.1"
                     /translation="MKTCWQILEIESTTQIDIIRQAYLARLPLCHPETDPQGFKALRQ
                     AYEEALRLAVNPVEEADDEEKDAAAEHEILRAFRALLDSESDRFQPSAWQKFIQQLNT
                     WNMEDVDQLRWPLCAVAIEARYLSLNCASLLAERLNWHSFNDSEGMDEEEREAFLEAI
                     QAGDCFDFLSLLEYPVALQNQTVEYYFALERCCRYHPDYVTAFMAMEGPWFIPDDVKL
                     HRKLLRWYSSVKTGMAELIPVAQQWQAENPESEDARYYQCAQRLYCGEGESLLADLCA
                     YRESYPSTQADNLLLQWSKRHCPDYFALLVMVIEARSMIDAQGQPLKYVPGESARTRL
                     LWAEILHSGKLSPLGQSFIESLFFKRKAWAWWKSRVGSETEQDSPLLDLYRVAEQVVL
                     EAFPKQEMLARLNTRLEGGDAHPLEAIITRMLLTKVKLEPEDEDVDEPTPENHEEKND
                     EDEKPQSITSIIKISLTVLVIGYAIGKIAMLFS"
     gene            complement(15294..16001)
                     /locus_tag="PN977_001114"
     CDS             complement(15294..16001)
                     /locus_tag="PN977_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HDI6041170.1"
                     /translation="MNKEEQYLLFALSTPMEILNQGCKPAHDSPKMYTGIKEFDLSSS
                     WGINNRDDLIQTIYQMTDDGHANDLAGLYLTWHRSSPEEWKALIAGGSERGLIYTQFV
                     AQTAMCCGEGGIKAWDYVRMGFLSRVGVLNKWLTEDESLWLQSRVYIRAHHYYHSWMH
                     YFSAYSLGRLYWQSSQCEDNASLREALTLYKYDSAGSRMFEELAAGSDRFYATLPWQP
                     LTVQPECPVTLKDVSDL"
     gene            16103..16657
                     /locus_tag="PN977_001115"
     CDS             16103..16657
                     /locus_tag="PN977_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HDI6041171.1"
                     /translation="MNKKLMYIFAIFIVAAITCISQPKKTTLRDKAMANYAFDYLSSP
                     GSLPFTTAATELSAIHGHSTSQYRLGEFYLHGSDGKPLDYTQARYWYEQSAEQENPRA
                     QSKLGWIYLKGLGVKPDTRKAILWYKEAAEQGYAHAQYTLGLIYRNGSGINVNHYESQ
                     KWLKLAAKQHYKNAERLLAGLPAH"
     gene            complement(16667..18094)
                     /locus_tag="PN977_001116"
     CDS             complement(16667..18094)
                     /locus_tag="PN977_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HDI6041172.1"
                     /translation="MKNCWKILEIEETTDVDIIRRAYLALLPSFHPETDPQGFKQLRQ
                     AYEDALRIAQSPAKSVWQPEEHEVAEHEILLAFRALLASDSERFLPSAWQRFIQQLNY
                     CSMEDIDELRWSLCTIAMNTAHLSFECVVLLAERLRWLQEENVGEIDEEELESFLYAI
                     AKGNVFNFQTILHLPVAVQNDTIDFYQMFARIWSSHPEWLTLYLAQHRAVIIPDDANL
                     HRNLLRWYSTSRLDIPELLDYARSWREAEPDNEDARYYEYAQRVYCGEGESLLAELCD
                     YWREYPSTQADALMLQWCRQHRVDYYPLVVMMIEARDLVNDQGKPLLYVPGDSARTRF
                     HLYEILSDEKLSALGRSLVEMVLHKGRKPRISLTRDTEHPLWPLYLVAKQLVQASQPT
                     EESLMPIVSRLDAEDRCPLEALMIRRLLIQAANFTEKQTVEPEPQPQSMPVDDGGPGC
                     LGVIKIIFYIFIFAGLIGKILHLFG"
     gene            complement(18091..18798)
                     /locus_tag="PN977_001117"
     CDS             complement(18091..18798)
                     /locus_tag="PN977_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HDI6041173.1"
                     /translation="MDMESQKILFALSTPMEIRNECCLPSHSSPKMYLGTRFFDLSSS
                     WRIDDRDDLLRTIYRMIDNGHAARLAGFYHCWFRYSPCEWRDYLAELNEQGQAYAQFV
                     ASTAECCGEGGIKAWDYVRMGFLSRMGVLNNWLSEEESLWIQSRIHLRALRYYSHWRQ
                     YFAGYTFGRQYWQSPEDDDLPLLREFLARKEYDDSGNDMFYQLFASDDAYYATLPWQP
                     LADYPTCPETLKDMSDL"
     gene            18962..19939
                     /locus_tag="PN977_001118"
     CDS             18962..19939
                     /locus_tag="PN977_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415177.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SEL1-like repeat protein"
                     /protein_id="HDI6041174.1"
                     /translation="MIFTSSCCDNLSIEKIVKRAEKGDSEAQYIVGFYYNRDSAIDSP
                     DDEKAFYWLKLAAEQGHCEAQYSLGRKYSEDKSRHKDNEQAIFWLKKAALQGHTFASN
                     ALGWILDRGEDPNYKEAVAWYQIAAESGMPYAQNNLGWMYRNGNGVAQDYALAFFWYK
                     QAALQGHSYAQDNLADLYKDGEGVAQNKTLAAFWYLKSAQQGNRHAQFQIAWDYNAGE
                     GVDQDYKQAMYWYLKAAAQESVGAYVNIGYMYKHGQGVEKDYQAAFEWFMKAAECNDA
                     TAWYNLAIMYHYGEGRPVDLRQALDLYRKVQASGTRDVSQEIREIEDLL"
     gene            complement(20010..20492)
                     /locus_tag="PN977_001119"
     CDS             complement(20010..20492)
                     /locus_tag="PN977_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon-containing protein"
                     /protein_id="HDI6041175.1"
                     /translation="MNKVAQYYRELVASLSERLRNGERDIDALVEQARERVIKTGELT
                     RTEVDELTRAVRRDLEEFAMSYEESLKEESDSVFMRVIKESLWQELADITDKTQLEWR
                     EVFQDLNHHGVYHSGEVVGLGNLVCEKCHFHLPIYTPEVLTLCPKCGHDQFQRRPFEP
                     "
     gene            20727..23309
                     /gene="leuS"
                     /locus_tag="PN977_001120"
     CDS             20727..23309
                     /gene="leuS"
                     /locus_tag="PN977_001120"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008456064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HDI6041176.1"
                     /translation="MQEQYRPEEIESKVQLHWDEKRTFEVTEDESKEKYYCLSMLPYP
                     SGRLHMGHVRNYTIGDVIARYQRMLGKNVLQPIGWDAFGLPAEGAAVKNNTAPAPWTY
                     DNIAYMKNQLKMLGFGYDWSRELATCTPEYYRWEQKFFTELYKKGLVYKKTSAVNWCP
                     NDQTVLANEQVIDGCCWRCDTKVERKEIPQWFIKITAYADELLNDLDKLDHWPDTVKT
                     MQRNWIGRSEGVEITFNVNDYDNTLTVYTTRPDTFMGCSYLAVAAGHPLAQKAAENNP
                     ELAAFIDECRNTKVAEAEMATMEKKGVDTGFKAVHPLTGEEIPVWAANFVLMEYGTGA
                     VMAVPGHDQRDYEFASKYGLNIKPVILAADGSEPDLSQQALTEKGVLFNSGEFNGLDH
                     EAAFNAIADKLTAMGVGERKVNYRLRDWGVSRQRYWGAPIPMVTLEDGTVMPTPDDQL
                     PVILPEDVVMDGITSPIKADPEWAKTTVNGMPALRETDTFDTFMESSWYYARYTCPEY
                     KEGMLDSKAANYWLPVDIYIGGIEHAIMHLLYFRFFHKLMRDAGMVNSDEPAKQLLCQ
                     GMVLADAFYYVGENGERNWVSPVDAIVERDEKGRIVKAKDAAGHELVYTGMSKMSKSK
                     NNGIDPQVMVERYGADTVRLFMMFASPADMTLEWQESGVEGANRFLKRVWKLVYEHTA
                     KGDVAALNVDALTEDQKALRRDVHKTIAKVTDDIGRRQTFNTAIAAIMELMNKLAKAP
                     TDSEQDRALMQEALLAVIRMLNPFTPHICFTLWQELKGEGDIDNAPWPVADEKAMVED
                     STLVVVQVNGKVRAKITVPVDATEEQVRERAGQEHLVAKYLDGVTVRKVIYVPGKLLN
                     LVVG"
     gene            23324..23905
                     /gene="lptE"
                     /locus_tag="PN977_001121"
     CDS             23324..23905
                     /gene="lptE"
                     /locus_tag="PN977_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HDI6041177.1"
                     /translation="MRYLATLLLSLAVLITAGCGWHLRDTTQVPSTMKVMILDSGDPN
                     GPLSRAVRNQLRLNGVELLDKETTRKDVPSLRLGKVSIAKDTASVFRNGQTAEYQMIM
                     TVNATVLIPGRDIYPISAKVFRSFFDNPQMALAKDNEQDMIVKEMYDRAAEQLIRKLP
                     SIRAADIRSDEEQTSTTTDTPATPARVSTTLGN"
     gene            23905..24936
                     /gene="holA"
                     /locus_tag="PN977_001122"
     CDS             23905..24936
                     /gene="holA"
                     /locus_tag="PN977_001122"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HDI6041178.1"
                     /translation="MIRLYPEQLRAQLNEGLRAAYLLLGNDPLLLQESQDAVRQVAAA
                     QGFEEHHTFSIDPNTDWNAIFSLCQAMSLFASRQTLLLLLPENGPNAAINEQLLTLTG
                     LLHDDLLLIVRGNKLSKAQENAAWFTALANRSVQVTCQTPEQAQLPRWVAARAKQLNL
                     ELDDAANQVLCYCYEGNLLALAQALERLSLLWPDGKLTLPRVEQAVNDAAHFTPFHWV
                     DALLMGKSKRALHILQQLRLEGSEPVILLRTLQRELLLLVNLKRQSAHMPLRALFDKH
                     RVWQNRRGMMGEALNRLSQPQLRQAVQLLTRTELTLKQDYGQSVWAELEGLSLLLCHK
                     PLADVFIDG"
     gene            24938..25579
                     /gene="nadD"
                     /locus_tag="PN977_001123"
     CDS             24938..25579
                     /gene="nadD"
                     /locus_tag="PN977_001123"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HDI6041179.1"
                     /translation="MKSLQALFGGTFDPVHYGHLKPVETLANLIGLTRVTIIPNNVPP
                     HRPQPEANSVQRKHMLELAIADKPLFTLDERELKRNAPSYTAQTLKEWRQEQGPDVPL
                     AFIIGQDSLLTFPTWYEYETILDNAHLIVCRRPGYPLEMAQPQYQQWLEDHLTHNPED
                     LHLQPAGKIYLAETPWFNISATIIRERLQNGESCEDLLPEPVLTYINQQGLYR"
     gene            25603..26214
                     /locus_tag="PN977_001124"
     CDS             25603..26214
                     /locus_tag="PN977_001124"
                     /EC_number="3.1.3.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin/alpha-ribazole phosphatase"
                     /protein_id="HDI6041180.1"
                     /translation="MRLWLIRHGETQANVDGLYSGHAPTPLTARGIEQAQNLHTLLHD
                     VSFDLVLCSELERAQHTARRVLSDRQLPVQIIPELNEMFFGDWEMRHHRDLMQEDAEN
                     YSAWCNDWQHAIPTNGEGFQAFSQRVERFIARLSEFQHYQNILVVSHQGVLSLLIARL
                     IGMPAEAMWHFRVDQGCWSAIDINQKFATLRVLNSRAIGVENA"
     gene            26474..26791
                     /gene="rsfS"
                     /locus_tag="PN977_001125"
                     /gene_synonym="rsfA"
     CDS             26474..26791
                     /gene="rsfS"
                     /locus_tag="PN977_001125"
                     /gene_synonym="rsfA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007373852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HDI6041181.1"
                     /translation="MQGKALQDFVIDKIDDLKGQDIIALDVQGKSSITDCMIICTGTS
                     SRHVMSIADHVVQESRAAGLLPLGVEGENSADWIVVDLGDVIVHVMQEESRRLYELEK
                     LWS"
     gene            26795..27262
                     /gene="rlmH"
                     /locus_tag="PN977_001126"
     CDS             26795..27262
                     /gene="rlmH"
                     /locus_tag="PN977_001126"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HDI6041182.1"
                     /translation="MKLQLVAVGTKMPDWVQTGFTEYLRRFPKDMPFELIEIPAGKRG
                     KNADIKRILDKEGEQMLAAAGKNRIVTLDIPGKPWDTPQLAAELERWKLDGRDVSLLI
                     GGPEGLSPACKAAAEQSWSLSALTLPHPLVRVLVAESLYRAWSITTNHPYHRE"
     gene            27293..29194
                     /gene="mrdA"
                     /locus_tag="PN977_001127"
     CDS             27293..29194
                     /gene="mrdA"
                     /locus_tag="PN977_001127"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-transpeptidase MrdA"
                     /protein_id="HDI6041183.1"
                     /translation="MKLQNSFRDYTAESALFVRRALVAFLGILLLTGVLIANLYNLQI
                     VRFTDYQTRSNENRIKLVPIAPSRGIIYDRNGIPLALNRTIYQIEMMPEKVDNVQQTL
                     DALRSVVDLTDDDIAAFRKERARSHRFTSIPVKTNLTEVQVARFAVNQYRFPGVEVKG
                     YKRRYYPYGSALTHVIGYVSKINDKDVERLNNDGKLANYAATHDIGKLGIERYYEDVL
                     HGQTGYEEVEVNNRGRVIRQLKEVPPQAGHDIYLTLDLKLQQYIETLLAGSRAAVVVT
                     DPRTGGVLALVSTPSYDPNLFVDGISSKDYSALLNDPNTPLVNRATQGVYPPASTVKP
                     YVAVSALSAGVITRNTTLFDPGWWQLPGSEKRYRDWKKWGHGRLNVTRSLEESADTFF
                     YQVAYDMGIDRLSEWMGKFGYGHYTGIDLAEERSGNMPTREWKQKRFKKPWYQGDTIP
                     VGIGQGYWTATPIQMSKALMILINDGIVKVPHLLMSTAEDGKQVPWVQPHEPPVGDIH
                     SGYWELAKDGMYGVANRPNGTAHKYFASAPYKIAAKSGTAQVFGLKANETYNAHKIAE
                     RLRDHKLMTAFAPYNNPQVAVAMILENGGAGPAVGTLMRQILDHIMLGDNNTDLPAEN
                     PAVAAAEDH"
     gene            29197..30309
                     /gene="mrdB"
                     /locus_tag="PN977_001128"
                     /gene_synonym="rodA"
     CDS             29197..30309
                     /gene="mrdB"
                     /locus_tag="PN977_001128"
                     /gene_synonym="rodA"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004885876.1"
                     /note="rod shape-determining protein RodA; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase MrdB"
                     /protein_id="HDI6041184.1"
                     /translation="MTDNPNKKTFWDKVHLDPTMLLILLALLVYSALVIWSASGQDIG
                     MMERKIGQIAMGLVIMVVMAQIPPRVYEGWAPYLYIICIILLVAVDAFGAISKGAQRW
                     LDLGIVRFQPSEIAKIAVPLMVARFINRDVCPPSLKNTAIALVLIFMPTLLVAAQPDL
                     GTSILVALSGLFVLFLSGLSWRLIGVAVVLVAAFIPILWFFLMHDYQRQRVMMLLDPE
                     SDPLGAGYHIIQSKIAIGSGGLRGKGWLHGTQSQLEFLPERHTDFIFAVLAEELGLVG
                     ILILLALYILLIMRGLWIAARAQTTFGRVMAGGLMLILFVYVFVNIGMVSGILPVVGV
                     PLPLVSYGGSALIVLMAGFGIVMSIHTHRKMLSKSV"
     gene            30320..31408
                     /gene="rlpA"
                     /locus_tag="PN977_001129"
     CDS             30320..31408
                     /gene="rlpA"
                     /locus_tag="PN977_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic peptidoglycan transglycosylase RlpA"
                     /protein_id="HDI6041185.1"
                     /translation="MRKQWLGICIAAGMLAACTSDDGQQQTVSVPQPAVCNGPIVEIS
                     GADPRFEPLNATANQDYQRDGKSYKIVQDPSRFSQAGLAAIYDAEPGSNLTASGEAFD
                     PTQLTAAHPTLPIPSYARITNLANGRMIVVRINDRGPYGNDRVISLSRAAADRLNTSN
                     NTKVRIDPIIVAQDGSLSGPGMACTTVAKQTYALPAPPDLSGGAGTSSVSGPQGDILP
                     VSNSTLKSEDPTGAPVTSSGFLGAPTTLAPGVLEGSEPTPAPQPVVTAPSTTPATSPA
                     MVTPQAASQSASGNFMVQVGAVSDQARAQQYQQQLGQKFGVPGRVTQNGAVWRIQLGP
                     FASKAEASTLQQRLQTEAQLQSFITTAQ"
     gene            31548..32759
                     /gene="dacA"
                     /locus_tag="PN977_001130"
     CDS             31548..32759
                     /gene="dacA"
                     /locus_tag="PN977_001130"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase DacA"
                     /protein_id="HDI6041186.1"
                     /translation="MNTIFSARIMKRLALTTALCTAFISAAHADDLNIKTMIPGVPQI
                     DAESYILIDYNSGKVLAEQNADVRRDPASLTKMMTSYVIGQAMKAGKFKETDLVTIGN
                     DAWATGNPVFKGSSLMFLKPGMQVPVSQLIRGINLQSGNDACVAMADFAAGSQDAFVG
                     LMNSYVNALGLKNTHFQTVHGLDADGQYSSARDMALIGQALIRDVPNEYSIYKEKEFT
                     FNGIRQLNRNGLLWDNSLNVDGIKTGHTDKAGYNLVASATEGQMRLISAVMGGRTFKG
                     REAESKKLLTWGFRFFETVNPLKVGKEFASEPVWFGDSDRASLGVDKDVYLTIPRGRM
                     KDLKASYVLNSSELHAPLQKNQVVGTINFQLDGKTIEQRPLVVLQEIPEGNFFGKIID
                     YIKLMFHHWFG"
     gene            32869..33132
                     /locus_tag="PN977_001131"
     CDS             32869..33132
                     /locus_tag="PN977_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbeD family protein"
                     /protein_id="HDI6041187.1"
                     /translation="MKTKLNELLEFPTPFTYKVMGQALPELVDQVVEVVQRHAPGDYT
                     PTVKPSSKGNYHSVSITINATHIEQVETLYEELGKIDIVRMVL"
     gene            33233..33874
                     /gene="lipB"
                     /locus_tag="PN977_001132"
     CDS             33233..33874
                     /gene="lipB"
                     /locus_tag="PN977_001132"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415163.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HDI6041188.1"
                     /translation="MYQDKILVRQLGLQPYEPISLAMHEFTDTRDESTLDEIWLVEHY
                     PVFTQGQAGKAEHILMPGDIPVIQSDRGGQVTYHGPGQQVMYVLLNLKRRKLGVRELV
                     TLLEQTVVNTLAELGIEAHPRADAPGVYVGEKKICSLGLRIRRGCSFHGLALNVNMDL
                     SPFLRINPCGYAGMEMAKISQWKPEATTNNIAPRLLENILALLNNPDFEYITA"
     gene            34134..35087
                     /locus_tag="PN977_001133"
     CDS             34134..35087
                     /locus_tag="PN977_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
                     /protein_id="HDI6041189.1"
                     /translation="MDSNNQIETCLSRKSSEGKPQIFTTLRNIDLNLLTIFEAVYVHK
                     GIVNAAKVLNLTPSAISQSIQKLRVIFPDPLFIRKGQGVTPTAFAMHLHEYISQGLES
                     ILGALDIEGSYDKQRTITIATTPSVGALVLPVIYRAIKSHYPQLLLRNPPTSDAENQL
                     SQFQTDLIIDNMFCTNRTVQHHVLFTDNMVLICREGNPLLSLEDDRETIDNAAHILLL
                     PEGQNFSGLRQRVQEMFPDRQISFTSYNILTIAALVANSDMLAIIPSRFYNLFSRCWP
                     LEKLPFPSLNEEQIDFSIHYNKFSLRDPILHGVIDVIRNAF"
     gene            35296..36261
                     /gene="lipA"
                     /locus_tag="PN977_001134"
     CDS             35296..36261
                     /gene="lipA"
                     /locus_tag="PN977_001134"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HDI6041190.1"
                     /translation="MSKPIVMERGVKYRDADKMALIPVKNVATEREALLRKPEWMKIK
                     LPADSTRIQGIKAAMRKNGLHSVCEEASCPNLAECFNHGTATFMILGAICTRRCPFCD
                     VAHGRPVAPDANEPVKLAQTIADMALRYVVITSVDRDDLRDGGAQHFADCITAIREKS
                     PQIKIETLVPDFRGRMDRALDILTATPPDVFNHNLENVPRIYRQVRPGADYNWSLKLL
                     ERFKEAHPEIPTKSGLMVGLGETNEEIIEVMRDLRRHGVTMLTLGQYLQPSRHHLPVQ
                     RYVSPDEFDEMKAEALAMGFTHAACGPFVRSSYHADLQAKGMEVK"
     gene            complement(36362..36565)
                     /gene="tatE"
                     /locus_tag="PN977_001135"
     CDS             complement(36362..36565)
                     /gene="tatE"
                     /locus_tag="PN977_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatE"
                     /protein_id="HDI6041191.1"
                     /translation="MGEISITKLLVVAALVVLLFGTKKLRTLGGDLGAAIKGFKKAMN
                     DDDAAAKKGADVDLQAEKLSHKE"
     gene            complement(36694..37482)
                     /locus_tag="PN977_001136"
     CDS             complement(36694..37482)
                     /locus_tag="PN977_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deaminated glutathione amidase"
                     /protein_id="HDI6041192.1"
                     /translation="MLVAAGQFAVTSVWEKNAEICASLMAQAAENDVSLFVLPEALLA
                     RDDHDADLSVKSAQLLEGEFLGRLRRESKRNMMTTILTIHVPSTPGRAWNMLVALQAG
                     NIVARYAKLHLYDAFAIQESRHVDAGNEIAPLLEVEGMKVGLMTCYDLRFPELALAQA
                     LQGAEILVLPAAWVRGPLKEHHWSTLLAARALDTTCYMVAAGECGNKNIGQSRIIDPF
                     GVTIAAASEMPALIMAEVTPERVRQVRAQLPVLNNRRFAPPQLL"
     gene            37578..37961
                     /gene="crcB"
                     /locus_tag="PN977_001137"
     CDS             37578..37961
                     /gene="crcB"
                     /locus_tag="PN977_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HDI6041193.1"
                     /translation="MLQLLLAVFIGGGTGSVARWLLSMRFNPLHQAIPLGTLAANLIG
                     AFIIGMGFAWFSRMTNIDPVWKVLITTGFCGGLTTFSTFSAEVVFLLQEGRFGWALLN
                     VFVNLLGSFAMTALAFWLFSASTAH"
     gene            complement(38015..38224)
                     /gene="cspE"
                     /locus_tag="PN977_001138"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             complement(38015..38224)
                     /gene="cspE"
                     /locus_tag="PN977_001138"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HDI6041194.1"
                     /translation="MSKIKGNVKWFNESKGFGFITPEDGSKDVFVHFSAIQTNGFKTL
                     AEGQRVEFEITNGAKGPSAANVIAL"
     gene            complement(38399..38959)
                     /gene="pagP"
                     /locus_tag="PN977_001139"
     CDS             complement(38399..38959)
                     /gene="pagP"
                     /locus_tag="PN977_001139"
                     /EC_number="2.3.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) palmitoyltransferase PagP"
                     /protein_id="HDI6041195.1"
                     /translation="MNVSKYVTIFFFVFIQLISVGKVFANADEWMTTFRENIAQTWQQ
                     PEHYDLYIPAITWHARFAYDKEKTDRYNERPWGGGFGQSRWDEKGNWHGLYAMAFKDS
                     WNKWEPIAGYGWESTWRPLADENFHLGLGFTAGVTARDNWNYIPLPVLLPLASVGYGP
                     ATFQMTYIPGTYNNGNVYFAWMRFQF"
     gene            39548..40933
                     /gene="dcuC"
                     /locus_tag="PN977_001140"
     CDS             39548..40933
                     /gene="dcuC"
                     /locus_tag="PN977_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HDI6041196.1"
                     /translation="MLTFIELLIGVVVIVGVARYIIKGYSATGVLFVGGLLLLIISAI
                     MGHKVLPSSQASTGYSATDIVEYVKILLMSRGGDLGMMIMMLCGFAAYMTHIGANDMV
                     VKLASKPLQYINSPYLLMIAAYFVACLMSLAVSSATGLGVLLMATLFPVMVNVGISRG
                     AAAAICASPAAIILAPTSGDVVLAAQASEMSLIDFAFKTTLPISIAAIIGMAIAHFFW
                     QRYLDKKEHISHEMLDVSEITTTAPAFYAILPFTPIIGVLIFDGKWGPQLHIITILVI
                     CMLIASILEFIRSFNTQKVFSGLEVAYRGMADAFANVVMLLVAAGVFAQGLSTIGFIQ
                     SLISIATSFGSASIILMLVLVILTMLAAVTTGSGNAPFYAFVEMIPKLAHSSGINPAY
                     LTIPMLQASNLGRTLSPVSGVVVAVAGMAKISPFEVVKRTSVPVLVGLVIVIVATELM
                     VPGTAAAVTGK"
     gene            complement(40974..41654)
                     /gene="dpiA"
                     /locus_tag="PN977_001141"
                     /gene_synonym="citB"
     CDS             complement(40974..41654)
                     /gene="dpiA"
                     /locus_tag="PN977_001141"
                     /gene_synonym="citB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component response regulator DpiA"
                     /protein_id="HDI6041197.1"
                     /translation="MTAPLTLLIVEDETPLAEMHAEYIRHIPGFSQILLAGNLAQARM
                     MIERFKPGLILLDNYLPDGRGINLLHELVQAHYPGDVVFTTAASDMETVSEAVRCGVF
                     DYLIKPIAYERLGQTLTRFRQRKHMLESIDSASQKQIDEMFNAYARGEPKDELPTGID
                     PLTLNAVRKLFKEPGVQHTAETVAQALTISRTTARRYLEYCASRHLIIAEIVHGKVGR
                     PQRIYHSG"
     gene            complement(41623..43281)
                     /gene="dpiB"
                     /locus_tag="PN977_001142"
     CDS             complement(41623..43281)
                     /gene="dpiB"
                     /locus_tag="PN977_001142"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase DpiB"
                     /protein_id="HDI6041198.1"
                     /translation="MLQLNENKQFAFFQRLAFPLRIFLLILVFSIFVIAALAQYFTAS
                     FEDYLTLHVRDMAMNQAKIIASNDSIISAVKTRDYKRLATIADKLQRDTDFDYVVIGD
                     RHSIRLYHPNPEKIGYPMQFTKPGALEKGESYFITGKGSIGMAMRAKTPIFDDDGKVI
                     GVVSIGYLVSKIDSWRAEFLLPMAGVFVVLLGILMLLSWFLAAHIRRQMMGMEPKQIA
                     RVVRQQEALFSSVYEGLIAVDPHGYITAINRNARKMLGLSSPGRQWLGKPIAEVVRPA
                     DFFTEQIDEKRQDVVANFNGLSVIANREAIRSGDDLLGAIISFRSKDEISTLNAQLTQ
                     IKQYVESLRTLRHEHLNWMSTLNGLLQMKEYDRVLAMVQGESQAQQQLIDSLREAFAD
                     RQVAGLLFGKVQRARELGLKMVIVPGSQLSQLPPGLDSTEFAAIVGNLLDNAFEASLR
                     SDEGNKIVELFLSDEGDDVVIEVADQGCGVPESLRDKIFEQGVSTRADEPGEHGIGLY
                     LIASYVTRCGGVITLEDNDPCGTLFSIYIPKVKPNDSSINPIDR"
     gene            43660..44718
                     /gene="citC"
                     /locus_tag="PN977_001143"
     CDS             43660..44718
                     /gene="citC"
                     /locus_tag="PN977_001143"
                     /EC_number="6.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415151.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[citrate (pro-3S)-lyase] ligase"
                     /protein_id="HDI6041199.1"
                     /translation="MFGNDIFTRVKRSENKKMAEIAQFLHENDLSVDTTVEVFITVTR
                     DEKLIACGGIAGNIIKCVAISESVRGEGLALTLATELINLAYERHSTHLFIYTKTEYE
                     ALFRQCGFSTLTSVPGVMVLMENSATRLKRYAESLKKFRHPGNKIGCIVMNANPFTNG
                     HRYLIQQAAAQCDWLHLFLVKEDSSRFPYEDRLDLVLKGTADIPRLTVHRGSEYIISR
                     ATFPCYFIKEQSVINHCYTEIDLKIFRQYLAPALGVTHRFVGTEPFCRVTAQYNQDMR
                     YWLETPTISAPPIELVEIERLRYQEMPISASRVRQLLAKNDLTAIAPLVPAVTLHYLQ
                     NLLEHSRQDAAARQKTPA"
     gene            44733..45029
                     /gene="citD"
                     /locus_tag="PN977_001144"
     CDS             44733..45029
                     /gene="citD"
                     /locus_tag="PN977_001144"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase acyl carrier protein"
                     /protein_id="HDI6041200.1"
                     /translation="MKINQPAVAGTLESGDVMIRIAPLDTQDIDLQINSSVEKQFGDA
                     IHTTILDVLARYNVRGVQLNVDDKGALDCILRARLEALLARASGIPALPWEDCQ"
     gene            45026..45934
                     /gene="citE"
                     /locus_tag="PN977_001145"
     CDS             45026..45934
                     /gene="citE"
                     /locus_tag="PN977_001145"
                     /EC_number="4.1.3.34"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415149.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (pro-3S)-lyase subunit beta"
                     /protein_id="HDI6041201.1"
                     /translation="MISASLQQRKTRTRRSMLFVPGANAAMVSNSFIYPADALMFDLE
                     DSVALREKDTARRMVYHALQHPLYRDIETIVRVNALDSEWGVNDLEAVVRGGADVVRL
                     PKTDTAQDVLDIEKEILRIEKACGREPGSTGLLAAIESPLGITRAVEIAHASERLIGI
                     ALGAEDYVRNLRTERSPEGTELLFARCSILQAARSAGIQAFDTVYSDANNEAGFLQEA
                     AHIKQLGFDGKSLINPRQIDLLHNLYAPTQKEVDHARRVVEAAEAAAREGLGVVSLNG
                     KMVDGPVIDRARLVLSRAELSGIREE"
     gene            45945..47477
                     /gene="citF"
                     /locus_tag="PN977_001146"
     CDS             45945..47477
                     /gene="citF"
                     /locus_tag="PN977_001146"
                     /EC_number="2.8.3.10"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase subunit alpha"
                     /protein_id="HDI6041202.1"
                     /translation="MTQKIEQSQRQERVAAWNRRAECDLAAFQNSPKQTYQAEKARDR
                     KLCANLEEAIRRSGLQDGMTVSFHHAFRGGDLTVNMVMDVIAKMGFKNLTLASSSLSD
                     CHAPLVEHIRRGVVTRIYTSGLRGPLAEEISRGLLAEPVQIHSHGGRVHLVQSGELNI
                     DVAFLGVPSCDEFGNANGYSGKACCGSLGYAMVDADNAKQVVMLTEELLPYPHNPASI
                     EQDQVDLIVKVDRVGDAAKIGAGATRMTTNPRELLIARSAADVIVNSGYFKEGFSMQT
                     GTGGASLAVTRFLEDKMRSRDIRADFALGGITATMVDLHEKGLIRKLLDVQSFDSHAA
                     QSLARNPNHIEISANQYANWGSKGASVDRLDVVVLSALEIDTQFNVNVLTGSDGVLRG
                     ASGGHCDTAIASALSIIVAPLVRGRIPTLVDNVLTCITPGSSVDILVTDHGIAVNPAR
                     PELAERLQEAGIKVVSIEWLRERARLLTGEPQPIEFTDRVVAVVRYRDGSVIDVVHQV
                     KE"
     gene            47481..48032
                     /gene="citX"
                     /locus_tag="PN977_001147"
     CDS             47481..48032
                     /gene="citX"
                     /locus_tag="PN977_001147"
                     /EC_number="2.7.7.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase holo-[acyl-carrier protein]
                     synthase"
                     /protein_id="HDI6041203.1"
                     /translation="MHLLPELASHHAVSIPELLVSRDERQARQHVWLKRHPVPLVSFT
                     VVAPGPIKDSEVTRRIFNHGVTALRALAAKQGWQIQEQAALVSASGPEGMLSIAAPAR
                     DLKLATIELEHSHPLGRLWDIDVLTPEGDILSRRDYSLPPRRCLLCEQSAAVCARGKT
                     HQLTDLLNRMEALLNDVDACNVN"
     gene            48007..48885
                     /gene="citG"
                     /locus_tag="PN977_001148"
     CDS             48007..48885
                     /gene="citG"
                     /locus_tag="PN977_001148"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase CitG"
                     /protein_id="HDI6041204.1"
                     /translation="MSMPATSTKTTKLATSLIDEYALLGWRAMLTEVNLSPKPGLVDR
                     INCGAHKDMALEDFHRSALAIQGWLPRFIEFGACSAEMAPEAVLNGLRPIGMACEGDM
                     FRATAGVNTHKGSIFSLGLLCAAIGRLLQLNQPVTPTTVCSTAASFCRGLTDRELRTN
                     NSQLTAGQRLYQQLGLTGARGEAEAGYPLVINHALPHYLTLLDQGLDPELALLDTLLL
                     LMATNGDTNVASRGGEGGLRWLQREAQTLLNNGGIRTPADLDYLRQFDRECIERNLSP
                     GGSADLLIITWFLAQI"
     gene            48935..50398
                     /gene="citT"
                     /locus_tag="PN977_001149"
     CDS             48935..50398
                     /gene="citT"
                     /locus_tag="PN977_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/succinate antiporter CitT"
                     /protein_id="HDI6041205.1"
                     /translation="MSLAKDNIWKLLAPLVVMGVMFLIPVPDGMPPQAWHYFAVFVAM
                     IVGMILEPIPATAISFIAVTICVIGSNYLLFDAKELADPAFNAQKQALKWGLAGFSST
                     TVWLVFGAFIFALGYEVTGLGRRIALFLVKFMGKRTLTLGYAIVIIDILLAPFTPSNT
                     ARTGGTVFPVIKNLPPLFKSFPNDPSARRIGGYLMWMMVISTSLSSSMFVTGAAPNVL
                     GLEFVSKIAGIQISWLQWFLSFLPVGIILLIVAPWLSYVLYKPEVTHSAEVAAWAGDE
                     LKTMGALSRKEWTLIGLVLLSLGLWVFGGEMIDATAVGLLAVSLMLALHVVPWKDITR
                     YNSAWNTLVNLATLVVMANGLTRSGFIDWFASTMSTHLEGFSPNATVIVLVLVFYFAH
                     YLFASLSAHTATMLPVILAVGKGIPGVPMEQLCILLVLSIGIMGCLTPYATGPGVIIY
                     GCGYVKSKDYWRLGAIFGVIYISMLLLVGWPILAMWN"
     gene            50512..51318
                     /gene="rna"
                     /locus_tag="PN977_001150"
     CDS             50512..51318
                     /gene="rna"
                     /locus_tag="PN977_001150"
                     /EC_number="4.6.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease I"
                     /protein_id="HDI6041206.1"
                     /translation="MKAFWRNAALLAVSLLPFSSANAVALQAKQYGDFDRYVLALSWQ
                     TGFCQSQHDRNRNERDECRLQTETTNKADFLTVHGLWPGLPKSVAARGVDERRWMRFG
                     CATRPIPNLPEARASRMCSSPETGLSLETAAKLSEVMPGAGGRSCLERYEYAKHGACF
                     GFDPDAYFGTMVRLNQEIKENEAGKFLADNYGKTVSRRDFDAAFAKSWGKENVKAVKL
                     TCQGNPAYLTEIQISIKADAINAPLSANSFLPQPHPGNCGKTFVIDKAGY"
BASE COUNT        12794 a        13221 c        12861 g        12666 t
ORIGIN      
        1 gcaccggatt ctgattccgg cattccgagg ttcgaatcct cgtaccccag ccaatttatt
       61 caagacactt accttgtaag tgcacccagt tggggtatcg ccaagcggta aggcaccgga
      121 ttctgattcc ggcattccga ggttcgaatc ctcgtacccc agccacatta aaaaagctcg
      181 cttcggcgag ctttttgctt ttctgcgtat attcaatgtc gaatgcgatg ttgatacagc
      241 taatttgaat ttggcccacg gttcagggca aaccagcctg gaagcacact caggcgcggt
      301 tttccccctc accctaaccc tcacccgagg ggcgagggga ccgtccgcac gcttttgtag
      361 ctttggtatc aaccgttacg ctacaaccct aacgcatatt tcagcgcctg acgtttcaac
      421 acgccagcac gctccgccgc cattaatccg agattacgca taaaacgcag tggtggcaga
      481 ttattgctga atccggcata aaacagatcc ataccgcttt gcataatgaa gttatccgcc
      541 atgcgccgca tctggtaacg cttgagcaca ggataactgg cccacgcttc gccgtagctg
      601 cgggcgttca ccagaacgtc aatcagggca tcgacatcac gataaccaag attcaccccc
      661 tgccccgcca gcggatggat ggtatgcgcg gcatctccca ccagtgccaa tcccggctga
      721 acatactgca acgcatggcg acgtgtcagc ggaaacgccc ccgcggcaag cggtgtcacg
      781 taacccagac gcgacgggaa atgcttcgcg atttccgcct gtagctgcgc catattcata
      841 ttctgcaact ggcgaatgcg cgccggagag tcataccaca ccagcgatgc ccagttatca
      901 aacaacggca gaaacgcacg cggtccgtcc ggggtaaatt gctgccaggt gctatcgccg
      961 ggatcgttct cgcactgtac gctaatcaac atacacgact gtgcatactg ccaggcatga
     1021 acgccaattc ccgccatctg ccgcacctgc gaatttgcgc cgtcggcacc aatcaccagc
     1081 ttcgcgcgga tcgtttcacc gcccttcagt tccagctcct gaagatcatt atggcgatgc
     1141 agcgcaatca gcgagcctgg cacacgtaac gttactttcg gatgcgcttc cagcgcctgc
     1201 cacagcgcct gttgcaggac agtgttttcc accatgtagc caagcaacgg tagcttaagt
     1261 tcagcggcgt caaacaccac atgcgccgtt tcccactccc acgtttccag tctgcggtaa
     1321 ggatggcaac gcatagcctg tactgcatcc cagaccccta accctttaag caatgatacc
     1381 gaagccgcac taatcgccga gatccgcacg tccggctggc tgtcagcgac aaacggtgca
     1441 ggttgggcat gttcgataac cattaccgaa aatccgtgct gagccagtcc cagcgccagt
     1501 gcgccgccga ccattcctcc gccgacaatg gcaatttccg ttggttgatt tgtcatggtc
     1561 aatcatcctg ttagcaatat gctttaagtt tacaggattt ttacccccaa gggctgataa
     1621 cgctcatact ggtcacaacg gcagcaaagc attacactat gcgccctgca ttcctggcta
     1681 ctatttcgca agagcaagtc gatgaccaaa aaactccata ttaaaacctg gggctgtcag
     1741 atgaacgagt acgattcatc gaagatggcc gatctgctgg atgccaccca cggctatcaa
     1801 ctgaccgatg tggcggaaga agcggatgtg ctgctgctga acacctgctc aatccgcgag
     1861 aaggctcagg aaaaagtctt ccatcagttg ggtcgctgga aactgttaaa agagaagaat
     1921 ccagacctga ttatcggcgt cggcggctgc gtggcatcgc aagaaggcga gcacattcgc
     1981 cagcgcgccc actatgtcga tattattttt gggccgcaaa cgctgcaccg tctgccggag
     2041 atgatcaact ccgtgcgcgg cgaccgcagc ccggttgtag atatcagctt cccggaaatc
     2101 gagaagtttg accgtctgcc ggaaccgcgc gccgaagggc cgaccgcgtt tgtctccatc
     2161 atggaaggct gcaataaata ttgcacctac tgcgtagtgc cttacacccg tggtgaagag
     2221 gtaagccgtc cgtccgacga tattctgttt gaaattgccc agcttgcggc tcagggcgtg
     2281 cgtgaagtca acctgctcgg tcagaacgtg aacgcctggc gtggtgagaa ctacgacggc
     2341 accaccggat cgtttgccga cctgctgcgt ctggttgcgg cgatcgacgg gatcgatcgt
     2401 attcgcttta ccaccagcca tccgatcgaa ttcaccgacg atatcatcga agtgtaccgc
     2461 gacacgccgg agctggtgag tttcctgcat ctgccggtac agagcggttc cgatcgcatt
     2521 ctgaacctga tgggacgtac ccatacagcg ctggagtaca aagcgatcat ccgtaaactg
     2581 cgtgcggcgc gtccggatat tcagatcagt tctgacttca tcgttggctt ccctggcgaa
     2641 accaccgaag acttcgagaa aacgatgaag ctgattgccg acgtcaattt cgacatgagc
     2701 tacagcttta tcttctctgc acgtccgggt acgccagccg ccgatatggt tgatgatgtt
     2761 ccggaagaag agaagaagca gcgtctgtat attctgcaag aacgtattaa tcagcaagcg
     2821 atggcatgga gccgccggat gctcggcacc acccagcgta ttctggtaga aggtacatcg
     2881 cgtaagagca tcatggagct ttccggtcgc actgaaaata accgcgtggt caactttgaa
     2941 ggcacgccag atatgatcgg taaattcgtc gatgtagaaa ttaccgacgt ctacccgaac
     3001 tctctgcgcg gtaaagtggt gcgtactgaa gatgaaatgg gtctgcgcgt ggcagaaacg
     3061 ccggaatcgg tgattgcccg tacccgcaaa gaaaacgacc ttggcgtggg ttattatcag
     3121 ccgtaattct caggcccgcc gttccggtgg gccttgtttt ttctctcttt atccccatct
     3181 ttatcgcaat gcttgcgcct ttttcctgtg gcgtgaataa tttccttata ggccggtgaa
     3241 tgttcagctt ccggcggcat acagataaga ggaacggttt gaacatagac actcgcgaaa
     3301 tcaccctgga gccagcagac aatgcgcgtc tgttgagcct gtgcggcccg tttgatgaca
     3361 acatcaagca gctcgaacgc cgtctcggca tcgagatcaa tcgccgcgat aaccacttta
     3421 aactgaccgg ccgtccgatt tgcgtcaccg ctgcggcaga cattctgcgt agcctgtatg
     3481 tcgatactgc cccgatgcgt ggtcagattc aggatatcga accggaacag atccaccttg
     3541 cgattaaaga agcgcgggta ctggagcaaa gcgcggagag cgtgccggag tacggcaaag
     3601 cggtcaatat caaaaccaaa cgcggcgtaa tcaagccgcg tacgccaaac caggcgcagt
     3661 acatcgccaa tattctcgac catgacatca ccttcggcgt tggcccggcg ggtacgggta
     3721 aaacctacct ggcagtggct gcggcagttg atgccctgga gcgtcaggaa attcgccgta
     3781 ttctgctgac tcgtccggcg gtagaagccg gtgagaaact gggcttcctg cctggcgatt
     3841 taagccagaa agtagacccg tatttgcgcc cactgtacga cgcgctgttt gaaatgctgg
     3901 gctttgagaa agtcgagaaa ctgattgagc gcaacgttat tgaagtcgcg ccgctggcct
     3961 atatgcgtgg tcgtacgctg aacgacgcgt ttatcattct cgatgagagc cagaacacta
     4021 ccatcgaaca gatgaagatg ttcctgaccc gtatcggttt taactcaaaa gcggttatca
     4081 ccggcgacgt cacgcagatc gacttgccgc gtaatactaa atcaggctta cgtcacgcta
     4141 tcgaagtgtt agccgatgtc gaagagatca gctttaactt cttccacagc gaagacgtgg
     4201 ttcgtcaccc ggtggtggcg cgtatcgtta acgcttatga agcctgggaa gaagcagaac
     4261 aaaaacgtaa agcggcgctg gcagcagaac gcaagcgcga agaacaggaa caaaaatgag
     4321 tcaggtgatc ctcgatttac aactggcatg tgaagataat tccgggttac cggaagagag
     4381 ccagtttcag acatggctga atgcggtgat cccgcagttt caggaagaat cggaagtgac
     4441 tattcgcgtg gtcgataccg ccgaaagcca cagtctgaat ctgacctatc gcggtaagga
     4501 taagccgacc aacgtgctct ccttcccgtt tgaagtgccg cctggcatgg aaatgtcgct
     4561 actgggcgat ctggttatct gccgtcaggt ggttgagaag gaagctcagg agcaaggcaa
     4621 accactggag gcgcactggg cgcatatggt ggtgcacggc agtctgcatt tgttaggtta
     4681 cgatcacatc gaagatgacg aagcagaaga aatggaagcc atcgaaacag agattatgct
     4741 tgctctgggc tatgaggatc cgtacattgc cgagaaagaa taatttgcca ggcatttgac
     4801 tggcagacgt ccccgccgtt ggtgattaac gacggcgttt tgattaacta acatgagaac
     4861 ccatacgacg ccatgagcga cgacaattca cacagtagtg acacgataag caacaagaag
     4921 ggatttttct ccctgttact cagccaactt ttccacggtg aaccgaaaaa ccgtgacgaa
     4981 ctgctggcgc tgatccgtga ttccgggcag aacgacctta tcgacgaaga tacgcgcgat
     5041 atgctcgaag gggtgatgga catcgcagac caacgcgtcc gcgacatcat gatcccccgc
     5101 tcccagatga ttaccctgaa acgcaaccag acgctggacg aatgccttga tgtcatcatt
     5161 gagtccgccc actcacgttt cccggtgatt agcgaagaca aagatcacat tgaagggatt
     5221 ctgatggcga aagacttgct gccgtttatg cgcagcgatg ctgaagcctt cagcatggac
     5281 aaagtgttac gtcaggcggt tgtcgttcct gaaagtaagc gcgtagaccg gatgctgaaa
     5341 gagtttcgct ctcagcgtta ccacatggcg atcgtcattg acgaattcgg tggagtttcc
     5401 ggtctggtga ccattgaaga catcctggaa ctgattgttg gtgagattga agacgagtat
     5461 gacgaagaag atgatatcga cttccgtcag ctgagtcgtc atacctggac cgtgcgcgca
     5521 ctggcttcta ttgaagactt caacgaagcg ttcggcaccc actttagcga tgaagaggtt
     5581 gacactatcg gtggtctggt gatgcaggca tttgggcatc ttccggcgcg tggcgaaacc
     5641 atcgacatcg acggttacca gttcaaagtg gcgatggccg acagtcggcg tattattcag
     5701 gttcatgtca aaatcccgga tgactcaccc cagccgaagc tggatgaata aaaccgaaac
     5761 tggatagata actacatggc ttttgcctca ttaattgaac gccagcgcat tcgcctgctg
     5821 ctggcgttat tattcggtgc ctgcggaacg ctggccttct ctccttacga cgtctggcct
     5881 gcggcgatta tttcgctgat ggggcttcag gcgttgacct ttaaccgccg tccactccag
     5941 tctgccgcta ttggcttttg ctggggattt ggcctctttg gcagcggtat taactgggtc
     6001 tatgtcagca tcgcgacctt tggcggaatg cctggcccgg ttaacatctt cctggtggta
     6061 ctgctggcgg cgtatttgtc gctgtatacc ggactgtttg ctggcgtgct gtcgcgtctg
     6121 tggccgaaaa ccacctggct gcgcgtggcg attgccgccc ctgctctctg gcaagtgacc
     6181 gagtttctgc gcggttgggt actaacaggc ttcccgtggt tacagttcgg ctatagccag
     6241 attgatggcc cgttaaaagg gctggcaccg ctaatgggcg tggaagccat taacttcctg
     6301 cttatgatgg ttagcggcct gctggcactg gcgttagtca aacgcaactg gcgtccgctg
     6361 gtggtggccg tcgtgctgtt tgccctaccc ttcccgctgc gttacatcca gtggtttacc
     6421 ccgcaaccgg agaaaaccat tcaggtttcg atggttcagg gcgatattcc gcaatcgctg
     6481 aaatgggacg gagaccagct acttaatacg ctgaagattt actacaacgc aacggcaccg
     6541 ctgatgggca aatcatcgtt gattatctgg ccggagtcgg cgataaccga tctggaaatt
     6601 aatcagcaac cgttcctcaa agcactggac ggtgagttgc gcgataaagg tagctcgctg
     6661 gtaaccggga ttgtcgacgc gcgtctcaat aagcagaacc gctacgatac ctacaacacc
     6721 atcatcacac tgggtaaagg tgcaccgtac agctacgaat cagccgatcg ctataacaaa
     6781 aaccatctgg tgccgtttgg cgagtttgtc ccgctcgagt cgattctgcg tccgttagca
     6841 ccgttctttg atctgccgat gtcgtcgttc agccgtgggc catatatcca gccgccgctg
     6901 tcggtaaatg gtattgagct tactgcggct atttgctacg agatcattct cggcgagcaa
     6961 gtgcgcgata acttccgccc ggataccgac tatctgctga ctatctccaa cgatgcgtgg
     7021 tttggtaagt ctattggtcc atggcagcac ttccagatgg cgcgaatgcg tgcgttggag
     7081 ctggcgcgcc cactgttgcg cagcaccaac aacggcatta cggcggtgat tggcccgcag
     7141 ggtgagattc aggcgatgat cccgcagttc acccgcgagg tgttaaccac taatgtgacg
     7201 ccgaccaccg gactcacacc gtatgcacgt accggcaact ggccgctgtg ggtactgacg
     7261 gcattgtttg gttttgccgc tgtgttgatg agtctgcgtc aacgacgtaa ataatccctt
     7321 cttatcgtgc cggaatacat cccgtatttc ggcacgctcg ccggatgtgg catcagtgcg
     7381 ttatccggat gacaaaattc ccctatccaa ctttggcaca tctattgctt tgttatacaa
     7441 ggcaaagctt gaacccgcat cagtacaacc cggttgcacc acagcaatcc accggtagca
     7501 ccaaaagagt gcattttaag aattttgctt cggaatggtg cggcgcatct cgcaaaaaca
     7561 aacaataaaa catcaatatc tttacattta gccaaactaa atgttaccta acaaacacaa
     7621 cactgcacaa taaagttgca gatgataaca acacaaacac tcacaacggg tatccatgcg
     7681 ttcttaacgc agaagataaa ggagttggat atgcaattac gtaaacctgc cacagcaatc
     7741 ctggccctgg cgctttccgc aggactggca caggcagatg acgccgcccc ggcagcaggc
     7801 agtacgctgg acaaaatcgc caaaaacggt gtgattgtcg tcggtcaccg tgaatcttca
     7861 gtacctttct cttattacga caatcagcaa aaagtggtgg gttactcgca ggattactcc
     7921 aacgccattg ttgaagcagt gaaaaagaaa ctcaacaaac cggacttgca ggtgaaattg
     7981 attccgatca cctcacaaaa ccgtattccg ctgctgcaaa acggcacttt cgattttgaa
     8041 tgtggttcta ccaccaacaa cgtcgaacgc caaaaacagg cggctttctc cgacactatt
     8101 ttcgtggtcg gtacgcgcct gctgacaaaa aaaggcggcg atattaaaga ttttgccgat
     8161 ctgaaagaca aagccgtcgt agtcacttcc ggcaccacct ctgaagtttt gctcaacaaa
     8221 ctgaatgaag agcaaaaaat gaacatgcgc atcatcagcg ccaaagatca cggtgactct
     8281 ttccgtaccc tggaaagcgg tcgtgccgtt gcctttatga tggatgatgc tctgctggct
     8341 ggtgaacgtg cgaaagcgaa gaaaccagac aactgggaaa tcgtcggcaa gccgcagtct
     8401 caggaagcct acggttgcat gttgcgtaaa gacgatccgc agttcaaaaa gctgatggat
     8461 gacactatcg ctcaggtgca gacctccggt gaagcggaaa aatggtttga taaatggttc
     8521 aaaaacccaa ttccgccgaa aaacctgaac atgaatttcg aactgtcgga cgaaatgaaa
     8581 gcactgttca aagaaccgaa tgacaaggca ctgaactaat tacaagaacc aggggcggaa
     8641 attccagccc tctcgattgt tacgtagcac ggacagacta tacgcctgat ggtcgttccc
     8701 catcgggcct gaaaaccgca atacgctggg taacaatctt cgagggtagc agttaacgct
     8761 gctaccctct tttttctgga gtagatttat gtctatagac tggaactggg gtattttttt
     8821 acaacaagcc ccgttcggca acaccaccta tcttggttgg atctggagtg gttttcaggt
     8881 gacgatcgct ttgtcgatct gcgcctggat tatcgctttc ctcgtcggtt catttttcgg
     8941 cattttacgt accgttccaa accgttttct ctccggtctg ggtacgttgt atgtcgaact
     9001 gttccgcaac gtgccgttga ttgtgcaatt ctttacctgg tatctggtga tcccggagct
     9061 gttgccggag aaaatcggca tgtggtttaa ggccgagctg gatcccaata ttcagttttt
     9121 cctttcatcc atgctctgcc tggggctgtt taccgccgcc cgtgtttgcg aacaggtacg
     9181 cgccgcgatt cagtcgttgc cgcgtgggca aaagaatgcc gcgctggcaa tgggtctgac
     9241 gctaccgcag gcttaccgct atgtactgct gcctaatgct tatcgcgtta tcgtcccgcc
     9301 gatgacctcc gagatgatga acctggtgaa aaactccgcc atcgcctcga ctatcggtct
     9361 ggtagatatg gcggcgcagg cgggtaaatt gctcgattac tcagctcatg cctgggagtc
     9421 gtttaccgcc attacgcttg cgtatgtgtt gattaacgcg gttatcatgc tggtaatgac
     9481 gctggttgaa cgtaaagttc gcctgcccgg caacatgggg ggcaaataat gtacgagttt
     9541 gactggagtt ccatagttcc ttccctgcca tatctgctcg acgggctggt gatcaccctg
     9601 aaaatcaccg tcacggcagt cgtgattggt attttgtggg gcaccatgct ggcggtgatg
     9661 cgcttgtcca gctttgcgcc cgtagcgtgg tttgccaaag cctacgttaa cgtattccgc
     9721 tcgattcctt tagttatggt tttgctgtgg ttttacctga ttgtgccggg ttttctgcaa
     9781 aacgtgctgg gattatcgcc aaaaaatgat atccgtttaa tctcggcgat ggtggcgttt
     9841 tcgatgtttg aagcggccta ctattcagag attatccgtg ccggtattca aagtatttcc
     9901 cgtggtcagt cgagcgccgc gctggcgttg gggatgaatc actggcagtc gatgaaactg
     9961 attattctgc cgcaggcgtt ccgcgcgatg gtgccgctgc tgctcactca gggcatcgta
    10021 ctgttccagg atacctcact ggtgtatgtg ttatctctgg ccgatttctt ccgtaccgcc
    10081 tcaaccattg gtgagcgtga tggtacgcag gtcgagatga tcctgtttgc cgggtttgtt
    10141 tatttcgtta ttagccttag cgcatcgttg ttggtcagct acttgaaaag aaggacagca
    10201 taatgattac cctgaaaaat gtttcaaaat ggtatggtca ctttcaggtg ctgaccgact
    10261 gctcaaccga agtgaaaaaa ggcgaagtgg tggtggtttg cggcccgtct ggttccggca
    10321 aatcaacgct gattaaaacc gtcaacggcc tcgaaccggt gcagcaaggt gaaatcaccg
    10381 tcgatggcat cgtggttaat gataagaaaa ccgatctggc aaagctgcgt tcccgcgtcg
    10441 ggatggtgtt tcagcatttc gagctgttcc cccatctgtc gattatcgaa aacctgaccc
    10501 tggcgcaggt gaaagtgctt aaacgcaata aagcgccggc tcgtgaaaaa gcattgaaat
    10561 tgctggagcg cgtcgggctt tccgcacatg ccaataagtt tccggcgcag ctttccggcg
    10621 gccagcagca gcgagtggcg atcgctcgcg cattgtgcat ggatcctatt gccatgctgt
    10681 tcgacgaacc aacgtcggcg ctggatccgg agatgatcaa cgaagtgctg gacgtgatgg
    10741 tcgaactggc gaacgaagga atgacgatga tggtggtgac ccacgaaatg ggctttgccc
    10801 gtaaagtggc gaatcgggtg atctttatgg acgagggtaa aattgtcgaa gactcgccga
    10861 aagacgcttt cttcgatgat ccgaaatcgg accgcgcaaa agacttcctc gcgaaaatcc
    10921 tgcattaatc actctggcgc gcatacccaa gtgcgcgcca tctcacgctt tatcgtttcg
    10981 ctcctcgtat ctgttacatc ctggcggtac ccttgtcgca aagaagcaca caacaaggag
    11041 caacaatggc actgccaatt ctgttagatt gcgacccagg tcatgacgac gctatcgcaa
    11101 tcgttctcgc cctcgcctca ccagagcttg atgtcaaagc aattacgtct tccgccggaa
    11161 accagacacc agaaaaaacc ttacgcaatg ttctgtgtat gctgaccttg cttaatcgca
    11221 ccgatattcc ggtagcaagc ggtgcggtaa aaccattaat gcgtgatttg attatcgcgg
    11281 acaatgtgca cggcgaaagc ggtctcgacg gcccggcatt accggaaccg acgttcgcac
    11341 cgcaaaactg tacggcggtg gagctgatgg cgaaaacgct gtgtgaaagt gaggaacctg
    11401 tcaccattgt ttctaccgga ccgcaaacta acgttgcctt gctgctcaat agccacccgg
    11461 aactgcatag caaaattgcc cgtatcgtga ttatgggcgg cgcaatgggg cttggtaact
    11521 ggacgcctgc ggctgaattt aatatttacg ttgacccgga agcggcagaa attgtcttcc
    11581 agtcagggat cccggtggtg atggccggtc tggatgttac tcacaaagca caaattcacg
    11641 ttgaggacac cgagcgtttc cgcgccattg gcaaccctgt ttcaaccatt gttgccgaac
    11701 tgctggattt cttcctcgaa tatcataaag acgaaaaatg gggctttgtc ggcgcaccgc
    11761 tgcatgaccc atgcaccatc gcctggctgt taaaaccgga gttgtttacc actgttgagc
    11821 gctgggttgg cgtggaaaca caagggaaat atacccaagg tatgacggtt gttgattatt
    11881 attatctgac tggcaataaa ccgaatgcca ctgtaatggt cgatgttgat cgtcagggct
    11941 ttgttgattt actggccgat cgtctgaaat tttacgctta acacacgtca ttgattaaga
    12001 tcaccacgcg ttacctgggt ggtcttaatc tgtttatctc tttttctttc aagatataaa
    12061 tgcaatggat aatgcagaac tcgccattgg tatcgatctc ggtactacca atagtttaat
    12121 tgccgtctgg aaagacggtg ccgcgcaatt aattccaaat aagttcggtg aatatttaac
    12181 accatccata attagcatgg atgaaaataa tcatatttta gtcggaaaac cggctgtatc
    12241 acggcgtact tcgcatccgg ataaaacggc agcgttattt aaacgtgcaa tgggcagtaa
    12301 taccaactgg cggttaggca gcgacacatt taacgcgcca gaactgtcct ctttggtatt
    12361 acgctcatta aaagaagatg ccgaagaatt tctgcaacgt ccgattaaag atgtggtgat
    12421 ctccgttccg gcttatttca gcgatgaaca acgcaagcat acccgtttag cagcggagtt
    12481 agccgggtta aatgcggtac gcttaattaa tgaacccaca gcagctgcga tggcatatgg
    12541 cctgcatacg caacaaaata cccgctcgct ggtttttgat ctcggtggcg gcacgtttga
    12601 cgtcacggtg ctcgaatacg ccacgccggt gattgaagtt cacgcctccg ctggcgacaa
    12661 ctttcttggc ggcgaagatt ttactcatat gctggtggat gaagttttaa aacgcgccgc
    12721 agtggctaaa aacatgctca acgaaagtga gctggcagcc ctgtacacca gcgtggaagc
    12781 ggcaaaatgt agcaatcaat taccgctgca aattagctgg cagtatcagg aagaaacgcg
    12841 tgagtgcgaa ttttacgaga acgaactgga agatttgtgg ttgccgctgc tcaatcgctt
    12901 gcgagtgccg attgaacagg cgttgcacga tgcacgtctg aagccaagtc aaatcgacag
    12961 tctggtgctg gttggcggcg catcacaaat gccgctggtg cagcgaatcg ccgtgcgtct
    13021 gtttggcaaa ttaccgtatc aaagttacga tccgagcacc attgtcgcgc tgggcgcagc
    13081 aatccaggcc gcctgccgct tacgcagtga agatattgaa gaagtgatcc tcactgatat
    13141 ttgcccctac tccttaggtg ttgaagttaa ccgccagggc gtttccggca ttttctcacc
    13201 gattattgaa cgaaatacca ctgtgccggt gtcgcgagta gaaacttact cgaccatgca
    13261 cccggagcag gattcaataa cgattaatgt ttatcaggga gaaaatcaca aagttaagaa
    13321 taatatactg gttgaatcct ttgatgttcc gctgaagaaa accggggctt atcagtctat
    13381 tgatattcgc tttagttatg atattaacgg attgcttgaa gttgacgtgc ttctggaaga
    13441 cggcagcgtt aagtccagag tcattaacca cagcccggta acgttaagta cccagcaaat
    13501 tgaagagagt cgggcacggt tatccgcatt aaaaatttat ccgcgcgata tgctcatcaa
    13561 ccgtaccttt aaagccaaac tggaagagtt atgggcgcgg gcgctgggtg acgagcgaga
    13621 agagattggc cgggtaatca ccgattttga tgcggcgctg caatcaaacg atatggcccg
    13681 cgtcgatgaa gttcgtcgac gggcgagcga ttatttggcc attgagatcc cttaaacacg
    13741 gctgccggat gcggcgtaaa cgccttatcc ggcctactcg taggtcggat aagatgcgtt
    13801 agcatcgcat ccgacgttga aggataagat gcgttagcct cacatctaac tgaacaacat
    13861 cgcgatttta ccgatcgcgt aacctatcac cagcaccgtt aagctgattt tgataatgct
    13921 ggtaatgctc tgtggttttt catcctcatc atttttttct tcgtggtttt caggtgttgg
    13981 ctcatcgaca tcttcatcct ccggctcgag tttcactttc gtcaacagca tccgcgtgat
    14041 aatggcctct aatggatgag catctccacc ttccagcctt gtattaagac gggccagcat
    14101 ctcttgtttc gggaacgctt ccagcacaac ctgttccgct acccgataca aatccagtaa
    14161 cggtgaatct tgttctgtct cgctaccgac tctcgatttc caccatgccc atgctttgcg
    14221 cttgaagaat aacgactcaa taaacgattg acctaacggc gataattttc cgctatgtaa
    14281 aatttccgcc cataacagcc gcgtccgggc gctctcacca ggaacatatt tcaacggttg
    14341 accttgcgca tctatcatgc ttcgcgcttc gataaccatc actaataacg cgaaataatc
    14401 cgggcaatga cgcttgctcc actgcaacaa caaattatca gcttgtgtag atgggtaact
    14461 ctcccggtat gcgcagagat cggcaagcag gctttccccc tcgccacagt acaaccgttg
    14521 cgcacactgg taataacggg catcttcgct ttctggattt tctgcttgcc actgttgggc
    14581 aacaggaata agttccgcca tacctgtttt caccgagctg taccagcgca acagtttgcg
    14641 atgtaacttg acatcatcag gaatgaacca cggaccttcc attgccataa acgcagtgac
    14701 atagtcagga tggtaacggc agcagcgttc cagcgcgaag taatactcaa cagtctggtt
    14761 ctgcaacgcg acgggatatt ccagaaggct aaggaaatcg aaacaatcac cagcctgaat
    14821 ggcctcaaga aaagcctccc tttcttcctc atccattcct tcgctgtcat taaacgaatg
    14881 ccagttcaaa cgctctgcca gcaaagaagc acaatttaat gaaagatatc gcgcttctat
    14941 agcgactgca cacagcggcc agcgtaattg atcgacatcc tccatgttcc aggtatttaa
    15001 ttgctgaata aatttctgcc aggcggaggg ctgaaaacga tcactttcag aatccagtaa
    15061 tgccctgaat gcacgtagta tttcatgttc agcggcggca tctttttctt catcatctgc
    15121 ttcctcgaca ggatttaccg ccagtcgcag cgcctcttca taggcctggc gtaatgcttt
    15181 aaacccctgc ggatcggttt cgggatgaca caacggtaag cgagcaagat aagcctggcg
    15241 gataatgtct atttgcgtcg tgctttcaat ttcgaggatt tgccaacatg ttttcatagg
    15301 tcgctcacat ccttaagcgt taccgggcat tcaggctgga cagttaatgg ctgccaggga
    15361 agcgtggcat agaaacggtc gcttcccgct gccagctctt cgaacatccg actcccggcg
    15421 ctatcgtatt tgtacagggt tagcgcctcg cgtagcgaag cgttgtcttc gcactgagaa
    15481 gattgccagt agagacgccc taacgaatag gcggaaaaat agtgcatcca gctgtgatag
    15541 taatggtgtg ctcgtatata gacgcgcgat tgcagccata aactctcgtc ttccgtcagc
    15601 cacttgttga gcacaccaac ccgtgataaa aaacccatcc gcacataatc ccaggctttg
    15661 attccccctt ccccacaaca catagcggtt tgggcaacga attgggtata aatcaaccct
    15721 ctctccgagc cgccagcaat taatgctttc cactcttcag gggaagagcg atgccatgtc
    15781 agatataagc cagctaaatc attggcatga ccatcatcgg tcatctgata aatagtctga
    15841 attaaatcat cacgattatt tattccccag gatgatgaga gatcgaattc ttttatcccg
    15901 gtatacattt ttggcgagtc atgcgcgggc ttgcaaccct gattaagtat ttccatgggc
    15961 gtagaaagcg caaaaagaag atattgttct tccttattca tttgttaata atcccttatg
    16021 tgcattaact gactactttt ttattattga tgagtataaa cttgacgctt tcaaaataaa
    16081 aagaaaatcg aagcattcac tcatgaataa aaaattaatg tatatattcg caatatttat
    16141 agttgcagca ataacctgta ttagccaacc caagaaaacg acgttgcgtg ataaagccat
    16201 ggcgaattat gcctttgatt atttaagctc accgggcagt cttccattca ccacggcagc
    16261 cacggagctt tccgcgattc atggtcactc aacgtcgcaa tatcgccttg gagaatttta
    16321 tcttcatggt agcgacggta aaccactgga ttatacacag gcgagatact ggtatgagca
    16381 atcagcggaa caggaaaatc cacgcgcgca aagtaaactg gggtggatct acctcaaagg
    16441 tctgggggtc aaacccgata cccgtaaagc aattctctgg tataaggaag cagctgaaca
    16501 agggtatgct catgctcaat atactttagg tttgatctac agaaacggct caggtattaa
    16561 tgttaaccat tatgaatctc aaaaatggtt aaaactggcc gccaaacaac attacaaaaa
    16621 cgcggaaaga ttacttgccg ggcttcccgc acattaacgt cgactgtcac ccgaacagat
    16681 ggagtatttt ccctatcaaa ccggcaaaga taaaaatgta gaaaataatt ttgatgacgc
    16741 ccaggcagcc cggcccacca tcgtcaacgg gcatcgactg cggttgcggc tccggttcga
    16801 cggtttgctt ctcggtaaaa tttgccgcct gaattaataa tcgacgaatc attaatgctt
    16861 ccagtggaca acgatcttct gcatcaaggc ggctgacgat cggcattaat gattcttctg
    16921 tcggttggct ggcctgaact aattgtttag caactaaata taatggccat aagggatgtt
    16981 ctgtatcacg cgtgagtgag atccgcggct tacgtccttt gtgcaaaacc atctcgacca
    17041 gagaacgccc cagcgcagag agtttttcat cactgagtat ttcgtataaa tgaaaacgcg
    17101 tacgagcgct gtcaccaggg acataaagta gcggttttcc ctgatcgtta accagatcac
    17161 gcgcttcaat catcatcacc actaacgggt aataatcgac ccgatgctga cggcaccatt
    17221 gcaacattaa agcatccgcc tgggtggagg gatactcgcg ccagtagtca caaagttctg
    17281 ccagcaggct ttcgccttct ccacaataga cgcgttgcgc gtattcataa taacgcgcat
    17341 cttcattatc aggttcagct tcccgccacg accgggcgta atccagaagt tcggggatat
    17401 ccagacggct tgtgctatac cagcggagta aatttctgtg caaatttgca tcatcgggga
    17461 taatcactgc gcgatgttgc gctaaataca atgtcagcca ttctggatgc gatgaccaaa
    17521 tccgagcgaa catttggtaa aaatcaatgg tgtcattttg tacggcaacg ggcagatgca
    17581 gaatggtctg gaagttaaaa acattcccct tcgcaatggc atataaaaag gattccagtt
    17641 cttcttcgtc tatttccccg acgttttcct cctgcaacca ccgcaatctt tctgctaata
    17701 acaccacgca ctcgaaggat aaatgggcag tgttcatggc tattgtgcac agcgaccagc
    17761 gtaattcatc aatatcttcc atcgagcaat aatttaattg ctgaatgaat cgctgccagg
    17821 cagagggcag aaaacgttcg ctatcagagg cgagtaacgc acgaaaggcg agcagaattt
    17881 catgttctgc aacttcatgt tcttctggtt gccaaacaga tttagcaggc gactgcgcaa
    17941 tccgtagcgc gtcctcatac gcctgacgaa gttgtttaaa accctgcgga tcggtttctg
    18001 gatgaaagga cggtaacagc gccagataag cgcggcggat aatatcgacg tcagtcgttt
    18061 cctctatttc gagaatcttc cagcaatttt tcataggtcg ctcatatcct taagcgtttc
    18121 cgggcatgtg gggtagtcag ccagtggttg ccagggtaag gtcgcgtaat atgcatcgtc
    18181 actggcaaat aattgataaa acatatcatt gccggaatcg tcgtattctt tacgcgctaa
    18241 gaattcacgt aataaaggca gatcatcatc ctcgggagac tgccaatatt gccgaccaaa
    18301 ggtataaccg gcaaagtatt gccgccagtg actataataa cgtagagccc gtagatgaat
    18361 gcgcgactgg atccacaggc tttcttcttc actcaaccag ttattaagta cgcccattcg
    18421 gcttaaaaaa cccatccgga cataatccca ggctttaatt cctccctcac cacagcattc
    18481 ggcggtgctg gcgacaaatt gcgcataagc ctgaccttgc tcatttaatt cagcaagata
    18541 gtcacgccat tcacatggcg aataacgaaa ccagcagtga taaaaccctg ccaaccgcgc
    18601 ggcatgaccg ttatcaatca ttcggtaaat tgtgcgcagc aggtcatcac ggtcatcaat
    18661 tctccaggag gaagaaaggt caaaaaagcg tgtacctaaa tacattttgg gggatgaatg
    18721 cgaaggtaaa cagcattcat tgcgtatttc catcggagtg gaaagtgcaa acaatatttt
    18781 ttgcgactcc atgtccatat aattcatcct taaatattca ttcaatgaat attaaccacg
    18841 ttgcgaggct catttattta tacttttctg acgcaacata atacgcataa aataaggctt
    18901 acgcaaaaat ggaacacata aaaatataat atgttttata tgcaaccata aggaaatgat
    18961 aatgattttt acgtcaagtt gctgcgataa tttatcaata gaaaagattg ttaaacgtgc
    19021 tgaaaaaggc gatagcgagg ctcaatatat tgttgggttt tattataatc gcgatagcgc
    19081 aattgactct ccggacgacg aaaaagcctt ctactggctg aagcttgccg ctgaacaagg
    19141 ccattgtgaa gcacaatatt ccttagggcg gaaatactcc gaggataaaa gccgtcataa
    19201 agataatgag caagccatct tttggctgaa aaaagctgcc ctacaaggcc atactttcgc
    19261 ttccaacgcc cttggctgga tactggatcg tggagaagac cccaactata aagaagcggt
    19321 tgcctggtat cagatagccg cggagagcgg aatgccttat gcgcaaaata atcttggatg
    19381 gatgtacaga aatggtaacg gagtcgcaca agactatgcg ctggcatttt tctggtacaa
    19441 acaagctgca ttacaaggcc atagttacgc gcaagacaac ctggccgatc tctataaaga
    19501 cggagaaggc gttgctcaaa acaagacact cgccgcattc tggtatttga aaagcgcaca
    19561 gcagggtaat cggcatgccc agtttcaaat tgcatgggat tataacgctg gcgaaggggt
    19621 ggaccaggac tataagcaag cgatgtactg gtatctgaag gctgccgctc aggaaagcgt
    19681 cggcgcttac gtcaacatcg gttatatgta taaacatgga caaggcgttg agaaagatta
    19741 tcaggccgcc tttgaatggt ttatgaaagc cgctgaatgc aatgacgcca ctgcctggta
    19801 taacctggcc attatgtatc actacggaga aggaagacct gtcgatctcc gacaggctct
    19861 cgacctgtat cgtaaagttc aggcatccgg taccagggat gtcagtcaag aaatccgtga
    19921 gattgaagat ttactgtaag agataaaaac aagggccggg gaattttatt cggcccatat
    19981 tctctttatt gctgtgcgaa atcagaccat taaggctcga acgggcgtct ctggaactgg
    20041 tcatgaccac atttcgggca tagcgtcagc acttccggtg tgtagattgg gagatggaaa
    20101 tgacatttct cgcagaccag atttcccagc cctaccactt ctccgctgtg ataaacccca
    20161 tgatgattga ggtcctggaa aacttcgcgc cattcaagct gcgttttatc ggtgatgtct
    20221 gccagctcct gccataagct ttctttaatc acccgcataa agacgctgtc agattcttct
    20281 ttcaggctct cttcatagct catggcgaac tcttccaggt cacgtctgac agctcgcgtc
    20341 agctcatcga cctcggttcg cgttaactcc cctgttttta ttacgcgctc gcgcgcctgc
    20401 tccaccagtg cgtcgatatc acgttcccca ttacgcaggc gttcgctcag tgacgcgacc
    20461 agttcacggt aatattgagc aaccttgttc atcgtttcat ctcccgggtc gttaactgac
    20521 tataataata gacgttcttt gttccttgct ttgctaatac gcccacaaac tctgtaaatt
    20581 aatgtatgaa taatccgggc atcatttcaa cgtcgagtgc gcgaaaggct gttttgacgc
    20641 ggacgtttgg gctatgctat gcggatctga aaaaccacct caacgctaca tttgtagccg
    20701 tattgaaaac aggaccactg gctgccatgc aagagcaata ccgcccggaa gagatagaat
    20761 ccaaagtaca gcttcattgg gatgagaagc gcacatttga agtaaccgaa gacgagagca
    20821 aagagaagta ttactgcctg tctatgcttc cctatccttc tggtcgacta cacatgggcc
    20881 acgtacgtaa ctacaccatc ggtgacgtga tcgcccgcta ccagcgtatg ctgggcaaaa
    20941 acgtcctgca gccgatcggc tgggacgcgt ttggtctgcc tgcggaaggc gcggcggtga
    21001 aaaacaacac cgctccggca ccgtggacgt acgacaacat cgcatacatg aaaaaccagc
    21061 tcaaaatgct gggctttggt tatgactgga gccgcgagct ggcaacctgt acgccggaat
    21121 actaccgttg ggaacagaaa ttcttcaccg agctgtataa aaaaggcctg gtatataaaa
    21181 agacttctgc ggtcaactgg tgcccgaacg accagaccgt actggcgaac gaacaagtta
    21241 tcgacggctg ctgctggcgc tgcgatacca aagttgaacg taaagagatc ccgcagtggt
    21301 ttatcaaaat caccgcttac gctgacgagc tgcttaacga tctggataaa ctggatcact
    21361 ggccagacac cgttaaaacc atgcagcgta actggatcgg tcgttccgaa ggcgtggaaa
    21421 tcaccttcaa cgttaacgac tatgacaaca cgctgaccgt ttacactacc cgtccggaca
    21481 cctttatggg ttgtagctac ctggcggtag cggcgggtca tccgctggcg cagaaagcgg
    21541 cggaaaataa tcctgaactg gcggccttta ttgacgaatg ccgtaacacc aaagttgccg
    21601 aagctgaaat ggcaacgatg gagaaaaaag gcgtcgatac tggctttaaa gcggttcacc
    21661 cattaacggg cgaagaaatt cccgtttggg cagcaaactt cgtattgatg gagtacggca
    21721 cgggcgcagt tatggcggtt ccgggtcacg accagcgcga ctacgagttt gcctctaaat
    21781 acggcctgaa catcaaaccg gttatcctgg cagctgacgg ctctgagcca gatctttctc
    21841 agcaagccct gactgaaaaa ggcgttctgt tcaactctgg cgagttcaac ggtcttgacc
    21901 atgaagcggc cttcaacgcc atcgccgata aactgactgc gatgggcgtt ggcgagcgta
    21961 aagtgaacta ccgcctgcgc gactggggtg tttcccgtca acgttactgg ggcgcgccaa
    22021 ttccgatggt aacgctggaa gacggcactg tgatgccgac cccggacgac cagctgccgg
    22081 tgatcctgcc ggaagatgtg gtgatggacg gtattaccag cccgattaaa gcagatccgg
    22141 agtgggcaaa aactaccgtt aacggtatgc cagcgctgcg tgaaaccgac actttcgaca
    22201 cctttatgga gtcctcctgg tactatgcgc gctacacttg cccggagtac aaagaaggta
    22261 tgctggattc caaagcggct aactactggc tgccggtgga tatctacatt ggtggtatcg
    22321 aacacgccat tatgcacctg ctctacttcc gcttcttcca caaactgatg cgtgatgcag
    22381 gcatggtgaa ctctgacgaa ccagcgaaac agttgctgtg tcagggtatg gtgctggcag
    22441 atgccttcta ctatgttggc gaaaacggcg aacgtaactg ggtttccccg gttgatgcta
    22501 tcgttgaacg tgacgagaaa ggccgtatcg tgaaagcgaa agacgcggca ggtcatgaac
    22561 tggtttatac cggcatgagc aaaatgtcca agtcgaagaa caacggtatc gacccgcagg
    22621 tgatggttga acgttacggc gcggacaccg ttcgtctgtt tatgatgttt gcttctccgg
    22681 cggatatgac tctcgaatgg caggaatccg gcgtggaagg ggctaaccgc ttcctgaaac
    22741 gtgtctggaa actggtgtac gagcacacag caaaaggtga tgttgcggca ctgaacgttg
    22801 atgcgctgac tgaagatcag aaagcgctgc gtcgcgatgt gcataaaact atcgctaaag
    22861 tgaccgatga tatcggccgt cgtcagacct tcaacaccgc tattgcggcg attatggagc
    22921 tgatgaacaa actggcgaaa gcaccgaccg atagcgagca ggatcgcgcc ctgatgcagg
    22981 aagcgctgct ggcggtaatc cgtatgctta acccgttcac cccacacatc tgcttcacgc
    23041 tgtggcagga actgaaaggc gaaggcgata tcgacaacgc gccgtggccg gttgctgacg
    23101 aaaaagcgat ggtggaagac tccacgctgg tcgtggtgca ggttaacggt aaagtccgtg
    23161 caaaaatcac cgttccggtg gacgcaacgg aagaacaggt tcgcgaacgt gctggccagg
    23221 aacatctggt agcaaaatat cttgatggcg ttactgtacg taaagtgatt tacgtaccag
    23281 gtaaactcct caatctggtc gttggctaag cgcgggagga agcgtgcgat atctggcaac
    23341 attgttgtta tctctggcgg tgttaatcac cgccgggtgt ggctggcatc tgcgtgatac
    23401 cacgcaggtt ccttccacta tgaaggtcat gatcctggac tcaggcgatc cgaacgggcc
    23461 attaagccgt gcggtgcgta atcagttacg tctgaatggt gtcgagttgc ttgataaaga
    23521 aaccacgcgt aaggacgttc catccctgcg tttgggtaaa gtgagcatcg cgaaagatac
    23581 cgcatcggta ttccgtaacg gtcaaacagc agagtatcag atgatcatga cggtgaatgc
    23641 gaccgtgttg atccccggcc gtgatatcta cccgattagc gccaaagtct tccgttcgtt
    23701 cttcgataac ccgcaaatgg cgttagcgaa agataacgaa caagacatga tcgtaaaaga
    23761 gatgtacgac cgtgctgccg aacagctgat tcgtaagctg ccaagcatcc gtgctgcgga
    23821 tattcgttcc gacgaagaac agacgtcgac cacaacggat actccggcaa cgcctgcacg
    23881 cgtctccacc acgctgggta actgatgatt cggttgtacc cggaacaact ccgcgcgcag
    23941 ctcaatgaag ggctgcgcgc ggcgtatctt ttacttggta acgatcctct gttattgcag
    24001 gaaagccagg acgctgttcg tcaggtcgct gcggcacaag gattcgaaga acaccacact
    24061 ttttccattg atcccaacac tgactggaat gcgatctttt cgttatgcca ggctatgagt
    24121 ctgtttgcca gtcgacaaac gctattgctg ttgttaccag aaaacggacc gaatgcggcg
    24181 atcaatgaac aacttctcac actcaccgga cttctgcatg acgacctgct gttgatcgtc
    24241 cgcggtaata aattaagcaa agcgcaagaa aatgccgcct ggtttactgc tcttgcgaat
    24301 cgcagcgtgc aggtgacctg tcagacaccg gagcaggctc agcttccccg ctgggttgct
    24361 gcgcgtgcaa aacagctcaa cttagaactg gatgacgctg caaatcaggt gctctgctac
    24421 tgttatgaag gtaacctgct ggcgctggcc caggcactgg agcgtttatc gctgctctgg
    24481 ccagacggca aactgacatt accgcgcgtt gaacaggcgg tgaatgatgc cgcgcatttc
    24541 accccttttc attgggttga tgctttgttg atgggaaaaa gtaagcgcgc gttgcatatt
    24601 cttcagcaac tgcgtctgga aggcagcgaa ccggttattt tgttgcgcac attacaacgt
    24661 gaactgttgt tactggttaa cctgaaacgc cagtctgccc atatgccact gcgtgcattg
    24721 tttgataagc atcgggtatg gcagaaccgc cggggcatga tgggcgaggc gttaaatcgc
    24781 ttaagccagc cgcagttacg tcaggccgtg caactcctga cacgaacgga actcaccctc
    24841 aaacaagatt acggtcagtc agtgtgggca gagctggaag ggttatctct tctgttgtgc
    24901 cataaacccc tggcggacgt atttatcgac ggttgatatg aaatctttac aggctctgtt
    24961 tggcggcacc tttgatccgg tgcactatgg tcatcttaaa cccgtggaaa cgctggcgaa
    25021 tttgattggt ctgacgcggg tcacaatcat ccctaataat gttcctccgc atcgtcccca
    25081 gccggaagcg aacagcgtgc agcgtaaaca catgcttgaa ctggcgattg ccgacaagcc
    25141 attatttact cttgatgaac gcgagctaaa gcgcaatgcc ccctcttaca ctgcgcaaac
    25201 actgaaagag tggcggcagg aacaaggacc ggacgtgccg ctggcgttta ttattggtca
    25261 ggattcattg ctgacctttc cgacctggta cgaatacgaa acgatactcg acaatgcaca
    25321 tttgatcgtc tgtcggcgtc caggttaccc acttgaaatg gcgcaaccgc aataccagca
    25381 atggctggaa gatcatttga cacataaccc ggaagatctt caccttcagc ctgccggtaa
    25441 aatttatctg gctgaaacgc cgtggtttaa catctcggcg accatcatcc gcgaacgttt
    25501 gcaaaacggt gaatcgtgtg aggatttatt gccggaaccg gtactgactt acattaacca
    25561 acaaggcttg tatcgctgat acctgtcgtt acggagggca tgatgcgact gtggttaatt
    25621 cgtcatggcg aaacgcaagc gaatgtcgat ggtctgtaca gcggtcatgc gcccactccc
    25681 ctgaccgcac gcggtattga gcaagcgcaa aatctgcata cgctgctaca tgatgtttcc
    25741 tttgacctgg ttttatgcag tgaactggaa cgggcacagc ataccgcgcg acgggttctc
    25801 agtgatcgcc agctccccgt gcaaatcatc cctgaactca acgaaatgtt ttttggcgac
    25861 tgggagatgc gacatcatcg cgacctcatg caagaagatg ccgaaaacta tagcgcgtgg
    25921 tgcaatgact ggcagcatgc tatccccacg aacggtgaag gatttcaggc attttcacaa
    25981 cgtgtggaac ggtttatcgc aaggcttagt gaatttcagc actatcagaa tattttagtc
    26041 gtcagccatc agggtgtact gagtctgtta atcgctcgtt taattggcat gcctgccgag
    26101 gctatgtggc attttcgcgt tgatcaaggg tgttggagcg ccattgatat caaccaaaaa
    26161 ttcgcgacgc tacgcgtcct caatagccgt gccatcgggg tcgagaatgc atgacttttc
    26221 tgttttttta cgggtaagcc gcaacgacca ttgacagccc cgggcaggct gatattctcc
    26281 gcagccagac tttttccgcc agacacgact ttgtagaaat tgttttacaa aaatggcgat
    26341 gcaatctgcg gcgcggggtg ggatgatagc ccactttcga aagccgattc ggcgacaatt
    26401 gtcccgaaat cgcctctggt tcaggtatac tgacagacca tttttatcta tttgattcac
    26461 ccagggggaa aacttgcagg gtaaagcact ccaggatttt gttatcgaca aaattgatga
    26521 cctcaaaggt caggacatca tcgccttaga cgttcagggc aaatccagca tcaccgactg
    26581 catgatcatc tgtacgggta cgtccagccg tcatgttatg tccattgctg accacgttgt
    26641 gcaggagtct cgcgcagcgg gcctgttacc gctcggcgta gaaggtgaaa acagcgccga
    26701 ctggattgtc gtggatttgg gcgatgtgat tgtccatgtc atgcaggaag agagccgtcg
    26761 cctgtatgaa ctggaaaaac tctggagtta atgcgtgaag ctgcaacttg tcgccgtggg
    26821 aacgaaaatg ccggactggg tacaaaccgg ttttaccgag tacctgcgtc gttttccgaa
    26881 agatatgccc ttcgagctga ttgaaattcc ggccggaaaa cgcggcaaga atgcggacat
    26941 caagcgcata ctcgacaaag agggtgagca gatgttggcg gccgcaggca aaaaccgcat
    27001 tgtcaccctc gatattccag gcaagccctg ggatacgccg cagttagccg ctgagctgga
    27061 acgctggaag ctggatggtc gcgacgtcag tctactgatt ggcgggcctg aagggttgtc
    27121 gcctgcctgt aaagcggcgg ctgagcagag ctggtcgctg tcggcgctta ccctccccca
    27181 tccgctggtt cgcgtgctgg tcgcagagag tctgtaccgg gcgtggagca tcaccaccaa
    27241 ccatccttat caccgtgagt gataagggag ccttgagtag aaaacgcagc ggatgaaact
    27301 acagaactct tttcgcgact atacggcaga gtccgcgctg tttgtgcgcc gggcgctggt
    27361 cgcctttttg gggattttgc tgctgaccgg cgtgcttatc gccaacctgt ataatctgca
    27421 aattgttcgc tttaccgact accagacccg ctctaatgaa aaccgcatta agctggtgcc
    27481 tatcgcaccc agccgcggca ttatctacga ccgtaacggt atccctctgg ctctcaaccg
    27541 cactatctac cagatagaaa tgatgccgga gaaagtcgat aacgtgcagc aaacgctgga
    27601 cgctttgcgc agcgtggttg atctgaccga tgatgatatt gctgcattcc gaaaagagcg
    27661 cgcacgttca caccgtttca cctccattcc ggtgaaaact aacctgaccg aagtacaagt
    27721 agctcgcttt gccgtcaacc agtaccgttt tccgggtgtt gaagttaaag gctataaacg
    27781 tcgttactat ccttacggtt cggcgttgac ccacgtcatc ggctatgtgt cgaaaatcaa
    27841 cgataaagac gtcgaacgcc tgaataatga cggcaaactg gccaactatg cggcaacgca
    27901 tgatatcggt aagctgggca ttgagcgtta ctatgaagac gtgctgcacg gtcagactgg
    27961 ttatgaagag gttgaagtta acaaccgtgg gcgtgttatc cgccagttaa aagaagtacc
    28021 accgcaagcc ggacacgata tttacctgac gctggatctc aaactccagc aatatattga
    28081 aacgctgctg gcgggtagcc gcgcagctgt ggtagtcacc gatccgcgta caggtggggt
    28141 gctggcgctg gtttccacgc ctagttatga cccaaacctg tttgttgacg gtatctccag
    28201 caaagattat tccgccttgc tgaacgaccc gaatacaccg ctggtgaacc gcgccacaca
    28261 aggggtttat cctcccgcgt ctacagttaa accctatgtg gcggtttcgg cattgagcgc
    28321 cggagtgatc acgcgcaata cgacgctgtt tgacccaggc tggtggcaac tgccaggttc
    28381 ggaaaaacgt tatcgtgact ggaaaaaatg gggccacggg cgtctgaatg tcacaagatc
    28441 gctggaagaa tctgcggata ccttcttcta tcaggtggcc tacgatatgg gaatcgatcg
    28501 cctctccgaa tggatgggta aattcggtta tggtcattac accggtatcg acctggcgga
    28561 agaacgttcc ggcaacatgc ctacccgcga atggaaacag aaacgcttta aaaaaccgtg
    28621 gtatcagggt gacaccattc cggttggtat cggtcagggt tactggacag cgaccccaat
    28681 ccagatgagt aaggcactga tgatcctgat taatgacggt atcgtgaagg ttccgcattt
    28741 gctgatgagc accgccgaag acggcaaaca ggtgccatgg gtacaaccgc atgaaccgcc
    28801 cgtcggcgat attcattccg gttactggga gctggcaaaa gacggtatgt acggtgttgc
    28861 taaccgccct aacggtacgg cgcataaata ctttgccagc gcaccgtaca aaattgcagc
    28921 aaaatccggt acggctcagg tcttcggcct gaaagcgaac gaaacctata atgcgcacaa
    28981 aattgccgag cgtttacgtg accacaaact gatgaccgcc tttgcgccat acaacaatcc
    29041 gcaagtggct gtcgccatga ttctggagaa cggtggcgcg ggtccggcgg ttggtacgct
    29101 gatgcgccag atcctcgacc acattatgct gggtgataac aacaccgatc tgcctgcgga
    29161 aaatccagcg gttgccgcag cggaggacca ttaatcatga cggataatcc gaataaaaaa
    29221 acattctggg ataaagtcca tctcgatccc acaatgctgc tgatcttact ggcattgctg
    29281 gtttacagcg ccctggttat ctggagcgcc agcggtcagg atattggcat gatggagcgt
    29341 aaaatcggcc agattgcgat gggtctggtc atcatggtgg tgatggcgca aattcctcca
    29401 cgcgtttatg aaggctgggc cccctatctc tatatcatct gtattatttt gctggtggcg
    29461 gtagatgctt tcggtgccat ctctaaaggc gctcaacgct ggctggacct cggtattgtt
    29521 cgttttcaac cgtcggaaat tgccaagata gccgtaccac tgatggttgc acgctttatc
    29581 aaccgcgacg tttgcccacc ttcattgaag aatacagcta tcgcgctggt gctgatattt
    29641 atgcccacgc tgctggtggc tgcacagcct gacctgggaa catcaatcct cgttgcgctt
    29701 tccggcctgt ttgtactgtt cctctctggc cttagctggc gtctgattgg cgtcgcagta
    29761 gtgctggtag cggcgttcat tccgattctg tggttcttcc tgatgcatga ttaccagcgc
    29821 cagcgcgtaa tgatgctcct ggacccggaa tcagacccac tcggcgcggg ctatcatatt
    29881 attcaatcta aaattgctat tggctccggc ggattacgcg gcaaaggctg gctgcacggt
    29941 actcagtcac agcttgaatt tctccccgaa cgccataccg actttatctt cgcggtactg
    30001 gcggaagagc tgggattagt gggcattttg attctgctcg ctctttacat tctgctgatc
    30061 atgcgcgggc tgtggatagc cgccagagcg caaaccacct ttggtcgcgt catggctggc
    30121 ggcttaatgc tgatattatt cgtttatgtc ttcgtaaata ttggtatggt aagcggtatt
    30181 ctgccggttg taggggttcc gctcccactg gtcagttatg gaggatcggc gctaattgtg
    30241 ctgatggctg ggttcgggat tgtaatgtca atccacaccc acaggaaaat gttgtcgaaa
    30301 agcgtgtaag aggtgcgcaa tgcgtaagca gtggctcggg atctgcatcg cggcaggaat
    30361 gctcgcggca tgtacaagcg atgatggtca gcaacagacg gtaagtgtac cgcagcctgc
    30421 ggtatgtaac ggccccatag ttgaaattag cggggcggac ccgcgtttcg aaccactgaa
    30481 cgcgacggca aatcaggatt accagcgcga cggtaaaagc tacaaaatcg tgcaggatcc
    30541 gtctcgattt agccaggcgg gactggcggc aatctatgat gccgaacccg gcagtaacct
    30601 gacggcctct ggcgaagctt tcgatccgac acagttgacg gcggcccatc caacgcttcc
    30661 gatccccagc tacgccagaa tcactaacct ggctaacggg cgaatgatcg tggtgcgcat
    30721 taatgatcgc ggtccttacg gcaacgaccg cgttatttcg ctttctcgcg cggcagctga
    30781 ccgtcttaac acgtcaaaca acaccaaagt tcgtatcgat ccgattattg tcgcccagga
    30841 tggttcgctt tctggtcctg gtatggcctg taccacagtt gccaaacaga cttacgccct
    30901 gcctgcacct cccgatttaa gcggcggcgc aggaacaagt tcagtgtctg gcccgcaggg
    30961 tgacattctt ccggtcagta attcgacgct aaaaagcgaa gatccgactg gcgcgccggt
    31021 aaccagcagc ggtttcctcg gcgcaccaac gaccttagcg cctggtgttc tggaaggcag
    31081 cgaaccaacg cctgcaccac agcccgttgt tacagctccg tcgacaacgc ctgcaacctc
    31141 gcctgcaatg gtgacaccgc aagccgcctc gcaaagcgcc agcggcaact ttatggtgca
    31201 agtcggggcc gttagcgatc aggctcgtgc gcaacagtac caacagcaac tgggacagaa
    31261 gttcggcgtc cccggtcgcg taactcaaaa tggcgcggtc tggcggatcc agcttggccc
    31321 attcgccagc aaagccgaag ccagtacctt gcagcaacgt ttgcaaaccg aagcccaatt
    31381 acagtcattt attaccaccg cgcagtagcg taaagcaggc atctgatgtg tcaatttctg
    31441 taaatgacgt taacaaagtc acgcggaaag tcagatgcct gccggtagtg catttgctat
    31501 agtagggcac tttttttaat tccatcacgg atgtcgtagt tcagaccatg aataccattt
    31561 tttccgctcg tatcatgaag cgcctggcgc tcaccacggc tctttgcaca gcctttatct
    31621 ctgctgcaca tgccgatgac ctgaatatca aaactatgat cccgggtgta ccgcagatcg
    31681 atgcggagtc ctacatcctg attgactata actccggcaa agtgctcgcc gaacagaacg
    31741 cagatgtccg ccgcgatcct gccagcctga ccaaaatgat gaccagttac gttatcggcc
    31801 aggcaatgaa agccggtaaa tttaaagaaa ctgatttagt cactatcggc aacgacgcat
    31861 gggccaccgg taacccggtg tttaaaggtt cttcactgat gttcctcaaa ccgggcatgc
    31921 aggttccggt ttctcagcta atccgcggta ttaacctgca atcgggtaac gatgcttgtg
    31981 tcgccatggc cgattttgcc gctggtagcc aggacgcttt tgttggcttg atgaacagct
    32041 acgttaacgc actgggcctg aaaaataccc acttccagac ggtacatggt ctggatgctg
    32101 atggtcagta cagctccgcg cgagatatgg cgctgatcgg ccaggcgttg atccgtgacg
    32161 taccgaatga atactcgatc tataaagaaa aagaatttac gtttaacggt attcgccagc
    32221 tgaaccgtaa cggcctgtta tgggataaca gcctgaatgt cgacggcatc aaaaccggac
    32281 acactgacaa agcaggttac aaccttgttg cttctgcgac tgaaggccag atgcgcttga
    32341 tctctgcggt gatgggcgga cgtactttta aaggccgtga agccgaaagt aaaaaactgc
    32401 taacctgggg cttccgtttc ttcgaaaccg ttaacccact gaaagtaggt aaagagttcg
    32461 cctctgaacc ggtttggttt ggtgattctg atcgcgcttc gttaggtgtt gataaagacg
    32521 tgtatctgac cattccgcgt ggccgcatga aagatctgaa agccagctat gtgctgaaca
    32581 gcagtgaatt gcatgcgccg ctgcaaaaga atcaggtcgt cggtactatc aacttccagc
    32641 ttgatggcaa aacgatcgaa caacgcccgc tggttgtgct gcaagaaatc ccggaaggca
    32701 acttcttcgg caaaatcatt gattacatta aattaatgtt ccatcactgg tttggttaaa
    32761 aatcgaacac ttgaaagtgt aatttccgtc cccatatact aagcatcagt aaaaaactcc
    32821 cgccttctgg cgggagttgc tatttaatta cgttacgccg gagctgacat gaaaaccaaa
    32881 cttaacgaac tgcttgaatt ccctactcct tttacttaca aagttatggg gcaggcgtta
    32941 cctgagctgg ttgatcaggt ggttgaagtg gtacagcgcc atgcgccagg tgactacacc
    33001 ccaacggtaa aaccaagcag caaaggcaac taccactcgg tatctatcac tatcaacgcg
    33061 actcatatcg agcaggttga aacactgtat gaagaactgg gcaaaatcga tattgtccgc
    33121 atggttctgt aactcgcttc tccgttaccc ggctcctggt cgggtaactc ccctctcctc
    33181 gctgtgatat acttttcccc cacttttact cattctccac ggagatgccg ttttgtatca
    33241 ggataaaatt cttgtccgcc agctcggtct tcagccttac gagccaatct ccctggctat
    33301 gcatgaattc accgataccc gcgatgaaag tacccttgat gaaatctggc tggtcgagca
    33361 ctatccggta tttacccaag ggcaggcagg aaaagcggag cacattttaa tgccgggtga
    33421 tattccggtg attcagagcg atcgcggtgg gcaggtgact tatcacgggc cggggcaaca
    33481 ggtgatgtat gtgttgctta acctgaaacg ccgtaaactc ggtgtgcgtg aactggtgac
    33541 cttgcttgag caaacagtgg tgaataccct ggctgaactg ggtatagaag cgcatcctcg
    33601 ggctgacgcg cctggtgtct atgtcgggga aaagaaaatt tgctcactgg gtttacgtat
    33661 tcgacgcggt tgttcattcc acggtctggc attaaacgtc aatatggatc tttcaccatt
    33721 tttacgtatt aatccttgtg ggtatgccgg aatggaaatg gctaaaatat cacaatggaa
    33781 acccgaagcg acgactaata atattgcacc gcgtttactg gaaaatattt tagcgctact
    33841 aaacaatcct gacttcgaat atattaccgc ttaattgcat acatcaatgg cccaatttac
    33901 actgggtcat tactcataat acctgctaat gctttacatc tccttcgcgt ttgtatattt
    33961 tctttatcac tagcagaaat gcgatgctac gccttgctcc gtaactcacc ccattgatta
    34021 acttattgaa atagttgatt atggtgagca aaagcgttcc gttccaatta gacataagat
    34081 tattgtaatt ttttttaata ttatttttcc atgaataaaa ttggagtaag tgcgtggata
    34141 gtaataatca aattgaaacc tgtttaagca gaaagtcgtc agaaggtaaa ccacaaatat
    34201 ttacaacctt acgaaatatt gatcttaacc ttctgactat ttttgaagct gtatatgtac
    34261 ataaagggat cgttaatgca gcgaaagtgc ttaatctgac accctcggca atcagtcagt
    34321 ctattcagaa actgcgcgtt atattccctg acccattgtt tattcgcaaa ggccagggtg
    34381 tcactcctac cgcatttgcg atgcatctac atgagtatat cagtcagggc cttgagtcca
    34441 ttcttggcgc gctggatatc gaaggaagct atgataagca acgaacgata actattgcga
    34501 ccaccccctc ggtcggagcc ctggtccttc ctgtcatcta tcgggcgatt aaatctcact
    34561 atccgcagct tttactgcgc aacccaccca ccagcgacgc ggaaaaccaa ctcagtcagt
    34621 ttcaaaccga tctcatcatc gataacatgt tttgcaccaa tcgtacggtg caacatcatg
    34681 ttctgttcac cgacaatatg gtgttaattt gccgtgaggg aaatccacta ctctctttag
    34741 aagatgacag agagactatc gacaacgctg cgcatatact cctgttaccg gaagggcaaa
    34801 atttcagcgg tctgcggcag agagttcaag agatgtttcc ggaccggcaa atcagtttca
    34861 ccagctacaa cattttgaca atcgctgcac tggttgccaa cagtgacatg ttagcgatta
    34921 ttcccagccg tttttataac ctgtttagcc gttgttggcc gctagaaaaa ctgccttttc
    34981 cgtccttaaa tgaggagcaa atagatttct ctatccacta caacaaattc agcctgcgtg
    35041 atcccatcct gcacggggta atagatgtta tccggaatgc attttgaaaa agtattgtta
    35101 cagccagatc acagcgaaga acgaaaaaat ggcacaatcc aacaacaatt ttacattttc
    35161 gcgaccgctt tggctgcttt caggtccgtt tcaatgatat actgccagtc gttaattcaa
    35221 aaatagttga taattacaac aatctattga attgaaacgc tttccttcgt aattcgcaac
    35281 tggaacacgc acgctatgag taaacccatt gtgatggaac gcggtgttaa ataccgcgat
    35341 gccgataaga tggcccttat cccggttaaa aacgtggcaa cagagcgcga agccctgctg
    35401 cgcaagccgg aatggatgaa aatcaagctt ccagcggact ctacacgtat ccagggcatc
    35461 aaagccgcaa tgcgcaaaaa tggcctgcat tctgtctgcg aggaagcctc ctgccctaac
    35521 ctggcggaat gcttcaacca cggcacagca acttttatga tcctcggcgc tatttgtacc
    35581 cgccgttgtc cgttctgtga cgttgcccac ggtcgcccgg tagctccgga tgccaatgaa
    35641 ccagtgaaac tggcgcagac cattgccgat atggcgctgc gttatgtggt tatcacctcc
    35701 gttgaccgtg atgacctgcg cgatggcggt gcccaacact ttgcggattg cattactgcc
    35761 attcgggaaa aaagcccgca aatcaaaatt gaaactctgg tgccggattt ccgcggtcgt
    35821 atggatcgtg ctctggatat tctgaccgcc acaccaccag atgtgttcaa ccataacctg
    35881 gaaaacgtac cgcgtattta ccgtcaggta cggcctggtg cagattacaa ctggtcgctg
    35941 aagctgctgg aacgctttaa agaagcgcat ccggaaatcc cgaccaagtc tggtctgatg
    36001 gtgggtctgg gtgaaaccaa tgaagaaatt attgaggtaa tgcgcgacct gcgccgtcat
    36061 ggtgtgacga tgttaacgct ggggcaatat ttgcagccaa gccgccatca cctgccggtt
    36121 caacgttacg ttagcccgga tgagttcgac gaaatgaaag ccgaagcgct ggcgatgggc
    36181 tttacccatg ctgcatgcgg tccgtttgtc cgctcgtctt accacgccga tttgcaggcg
    36241 aaagggatgg aagttaagta atcctgtaat aattacttac atctgtctga taaaaaaccc
    36301 gctttttgaa gcgggttttt tgtatcgaac aagatattga gggagcgtcc tgctcgccac
    36361 gtcactcttt atgagagagc ttttcagcct gaagatcaac gtctgcgcct tttttcgccg
    36421 cagcatcgtc atcattcatc gccttcttga accctttaat ggccgctcca aggtctccgc
    36481 ccagcgtacg taacttctta gtcccaaaca gcagaacgac cagcgccgca actaccagca
    36541 gtttggtaat actaatctca cccatagata ccttcttgac atataaccgg caacagaacg
    36601 cgccttaaaa accagagaat attaacggtc attcgacgcg cgcacacaat agcaatctgt
    36661 aacaaggtga atcaagcgcc gagtaaaaaa acatcataat aattgcggcg gcgcaaagcg
    36721 acggttgttt aagacgggca gttgcgcgcg cacctgacgc acacgttcgg gcgtcacttc
    36781 cgccataatg agtgcaggca tttctgacgc tgccgcaatg gtgacgccaa agggatctat
    36841 aatccggctt tgcccgatat ttttgttccc gcactccccc gccgccacca tataacaggt
    36901 ggtatccagc gcacgagcgg caagcaacgt tgaccagtga tgctctttga gcggcccacg
    36961 aacccaggca gcaggaagta ccaggatttc agctccctgt aatgcctgtg ccagcgccag
    37021 ctctggaaag cgtaagtcat aacaggtcat cagaccgacc ttcatccctt ccacctccag
    37081 taacggagct atttcattac cagcatcaac atggcgtgat tcctgaatgg caaatgcatc
    37141 atagagatga agtttggcat aacgggcgac gatgtttcct gcctgtagcg ccaccagcat
    37201 attccatgcg cgccccggcg ttgaaggaac atgaatcgtc agaattgtcg tcatcatgtt
    37261 acgtttactt tctcgacgta aacgtccgag gaattcgcct tccagcagct gtgctgattt
    37321 aaccgataga tctgcatcat gatcatcacg cgccaacaat gcttccggca ggacaaacag
    37381 cgaaacgtcg ttttccgccg cctgcgccat caacgaggca caaatctcag cgttcttttc
    37441 ccacacagat gtcacagcaa actgtcctgc ggcaaccagc attgccacct cctcctgtca
    37501 gagtcgtaaa attcgccaac ggcgattatt cggcgttaca cttatcactc atacaaatca
    37561 aatagcagga ttttgcagtg ttacaacttc ttttagcagt ttttataggc ggtggtacgg
    37621 gaagcgtggc gagatggctg ttaagtatgc gatttaaccc gctgcatcag gcgattccgt
    37681 tggggacgct ggcagccaac ctgattgggg cattcatcat aggaatggga ttcgcctggt
    37741 tcagcaggat gacgaacatt gatcccgtgt ggaaagtatt aatcaccacc ggattttgtg
    37801 gcggtctaac aaccttctca acattttcgg cagaagtggt gtttttgtta caagagggtc
    37861 gctttggctg ggcattactg aacgttttcg tcaaccttct ggggtctttt gccatgaccg
    37921 cactggcttt ctggctgttt tcggcctcaa ccgcacacta aagaccaaaa aaaacccgct
    37981 gattaagcgg gttttgaatt cttgctgacg tatcttacag agcgattacg tttgcagcag
    38041 aagggccttt ggcaccgtta gtgatttcga actctacgcg ctgaccttca gcaagagttt
    38101 taaaaccatt agtctggatt gcagagaagt gtacgaacac gtctttgctg ccgtcttccg
    38161 gagtaatgaa accgaatcct ttggactcat taaaccactt aacgttacct ttaatcttag
    38221 acatcaaaat tacctttaca tgaaaaatag acacaaatgc tgtgtcggtt accagtacac
    38281 caattgtggt acgctttgtc cagtgcaact ttactaaaaa gtgataaaag tcgcgttatt
    38341 tttgatgtta attattttag tggttgattt taaagaattt actaaaactt catttgtctc
    38401 aaaactgaaa gcgcatccag gcaaagtaca cattgccatt gttgtaggta cccggaatgt
    38461 aggtcatctg aaacgtcgcg gggccatacc ccacagaggc caatggcagt agaaccggga
    38521 gagggatgta attccagtta tcgcgtgccg ttacgccagc ggtgaatccc aggcctaaat
    38581 gaaaattttc atctgccagc ggtcgccagg tactttccca tccgtatccg gcaatcggtt
    38641 cccatttgtt ccacgagtcc ttaaatgcca tggcatacag gccatgccag tttccttttt
    38701 cgtcccaacg cgactgacca aaaccgccac cccacggtcg ctcgttatag cgatcggttt
    38761 tttctttgtc ataggcaaaa cgcgcatgcc aggtgatggc aggaatatat aaatcataat
    38821 gttctggctg ttgccaggtt tgtgcaatat tttctctaaa cgttgtcatc cactcatcag
    38881 cgttagcaaa aactttacca acactgatta actgaataaa aacaaagaaa aatatagtga
    38941 catatttact cacgttcatt tgtgaccatt aaacatttat caaaaatcta ctactagcat
    39001 agcaaagcta caattaacat aaccttaata gccccaacat aaaaaataat ctgaatagct
    39061 gctctattta tttccgcttg tttaagttaa atatttccgc acatcgatta aagatgatgc
    39121 gtcgttttct gcgatgggaa tagtcaaaaa agaaaaacca agtctttttt gatgacaaaa
    39181 tgcaatcaag gaaaaattat tttatttttt aacgagatac aacaatcatc ttaacgaagt
    39241 atataatata tatcgtcaac ccatgctcat attagattat tttttgagcg catggccgca
    39301 gctaacacca ttgcaattaa caaatttgca tcaatccacc atcaatttac acacattatt
    39361 atgtgatatt tgccaaccgc taaatatgcg ttttgttatc tacgtataaa aacagcaact
    39421 tcaatgtctt aatggcagtt tttcttgatt ttaatcagca ttcaccgcca atttattggg
    39481 catatttttt ccttagtttt taggaatttt ttattattta ctttggggcc tggagacagg
    39541 aaaaattatg ctgacattca ttgaactcct tattggggtt gtggttattg tgggtgtagc
    39601 tcgctacatc attaaagggt attccgccac tggcgtgtta tttgtcggtg gcctgttatt
    39661 gctgattatc agtgccatta tggggcacaa agtgttaccg tccagccagg cttcaacagg
    39721 ctacagcgcc acggatatcg ttgaatacgt taaaatattg ctaatgagcc gcggcggcga
    39781 cctcggcatg atgattatga tgctgtgtgg ctttgccgct tacatgaccc atatcggcgc
    39841 gaatgatatg gtggtcaagc tggcgtcaaa accattgcag tatattaact ccccttacct
    39901 gctgatgatt gccgcctatt ttgtcgcctg tctgatgtct ctggccgtct cttccgcaac
    39961 cggtctgggt gttttgctga tggcaaccct gtttccggtg atggtaaacg ttggtatcag
    40021 ccgtggtgca gctgctgcta tttgtgcctc cccggcggcg attattctcg caccgacttc
    40081 aggggatgtg gtgctggcgg cgcaggcttc cgaaatgtcg ctgattgact tcgccttcaa
    40141 aacgacgctg cctatctcaa ttgctgcaat tatcggcatg gcgatcgccc acttcttctg
    40201 gcaacgttat ctggataaaa aagagcacat ctctcatgaa atgttagatg tcagtgaaat
    40261 caccactact gctcctgcgt tttatgccat tttgccgttc acgccgatca tcggagtgct
    40321 gatttttgac ggcaaatggg gtccgcaatt acacatcatc actattctgg tgatttgtat
    40381 gctgattgcc tccattctgg agttcatccg cagctttaat acccagaaag ttttctctgg
    40441 tctggaagtg gcttatcgcg ggatggccga tgcgtttgct aacgtggtga tgctgctggt
    40501 tgccgctggg gtattcgctc aggggcttag caccatcggc tttattcaaa gtctgatttc
    40561 tatcgccacc tcgtttggtt cggcgagtat catcctgatg ctggtattag tgatcctgac
    40621 aatgctggcg gcagtcacga ccggttcagg caatgcgccg ttttatgcgt ttgttgagat
    40681 gatcccgaaa ctggcgcact cttccggcat taacccggcg tatttgacta tcccaatgct
    40741 gcaggcgtca aacctcggcc gtaccctgtc acccgtttct ggcgtagtcg ttgcggttgc
    40801 cgggatggcg aaaatctcac catttgaagt cgtaaaacgc acctcggtgc cggtgcttgt
    40861 tgggctggtg attgttatcg ttgctacaga gctgatggtg ccaggaacgg cagccgcggt
    40921 cacaggcaag taaatagtca tgccggaggc aagtttcctc cggctttatc atgtcaccca
    40981 ctgtggtata tgcgttgcgg tctgccaact ttgccgtgaa caatttcagc aataatcaga
    41041 tggcggctgg cgcaatattc aagataacgt ctggcagtgg tgcggctgat agtcagtgcc
    41101 tgcgccaccg tttctgccgt atgttgcaca ccaggctctt taaacagttt tcgcactgcg
    41161 tttagcgtca aggggtcaat gccggtcggc agttcgtctt tgggttcacc gcgagcatag
    41221 gcattaaaca tctcatcaat ttgcttctgg ctggcgctat caatactttc cagcatatgt
    41281 ttacgctggc ggaaacgggt tagcgtttgc cccagccgtt cataggcaat gggcttaatg
    41341 agataatcaa atacaccaca acgtacggct tcagacaccg tttccatatc gctggctgca
    41401 gtggtaaaca ccacgtcgcc gggataatgc gcctgcacca gttcatgcag taaattaatc
    41461 cctctaccgt caggaagata gttatcgagc aagattagcc ccggcttaaa acgctcgatc
    41521 atcattcggg cctgcgccag gtttcccgcc agtaatatct gactgaatcc gggaatgtga
    41581 cgaatatatt ccgcatgcat ctctgccagc ggcgtttcgt cctcaacgat caatagggtt
    41641 aatggagctg tcattaggtt tcactttcgg aatatagatt gaaaataagg taccgcaggg
    41701 atcattatct tcgagagtga taacaccacc gcagcgcgtt acgtagctgg caatcaagta
    41761 caacccaatg ccatgttcgc cgggctcgtc agcacgcgta ctgacaccct gctcaaatat
    41821 tttgtctcgt agagactctg gaacgccgca gccctgatcg gcgacttcaa tcaccacatc
    41881 atcgccttca tcgctgagga ataattcaac gatcttgttt ccttcatcgc tacgtaggct
    41941 ggcttcgaag gcgttatcaa gtaaattgcc aacaatggct gcaaactcgg tgctgtccag
    42001 tcctggcggc agttgcgaaa gctgacttcc ggggacgatg accattttta gccccagttc
    42061 gcgggcgcgc tgcactttac caaaaagcag ccccgccacc tggcgatcgg caaacgcctc
    42121 gcgcaggcta tcaataagct gttgctgggc ctgagactcc ccctgcacca tcgccagcac
    42181 gcgatcatac tctttcatct gcaacagacc attgagcgtc gacatccaat tgagatgctc
    42241 gtgtcgcaat gtgcgcaggc tctcgacgta ttgtttaatt tgcgtcagtt gcgcattgag
    42301 ggtggatatt tcgtctttac tacgaaagct gataatggcc cccagcaaat catcaccaga
    42361 acgaatagct tcccggttgg caataacgct cagaccgtta aagttcgcca ccacatcctg
    42421 acgtttttca tcaatctgtt cggtaaagaa atcggcgggc ctgaccactt cagcaatggg
    42481 tttacccaac cattgccgtc cgggggaact cagacccagc atctttcttg cgttacgatt
    42541 gatggcggta atgtaaccat gcggatccac cgcaatgagc ccttcataaa ccgaactaaa
    42601 cagcgcctct tgctgacgga ccacgcgtgc gatttgcttt ggctccatgc ccatcatctg
    42661 ccgacggata tgcgcggcca ggaaccacga tagcaacatc agaatcccta acagcacgac
    42721 aaacacgcca gccatcggta ataaaaactc agcccgccag ctatcgattt tactcaccag
    42781 gtagccaatc gacaccacgc cgatgacttt tccatcgtca tcaaagattg gcgttttggc
    42841 acgcatcgcc atgccaattg accctttccc ggtgatgaag tagctctccc ctttctccag
    42901 cgcgcccggt ttggtgaact gcataggata accaattttc tccggattag gatggtaaag
    42961 acgaatcgag tgccgatcgc caatcaccac ataatcgaaa tcagtatctc tttgtaattt
    43021 gtcagcgatg gtcgccagcc tcttgtagtc acgcgttttc actgcgctga tgatactgtc
    43081 attagaagca ataatttttg cctgattcat tgccatgtcg cgcacatgaa gcgttaaata
    43141 gtcctcaaaa ctggctgtaa aatattgcgc cagggctgca atgacaaata ttgagaacac
    43201 cagaatcagc aaaaagatac gcagcggaaa tgccagtctt tggaaaaatg caaactgttt
    43261 attctcgtta agctgcaaca ttgttttcct tgcgggttgt cccgagcctc tttgacggat
    43321 gtaattaaaa tgttgaatca aaatacatag atattaaata tgatcgtggc aaattttatt
    43381 cattaaatca attaaatcac ttaaacctgc gggtcatttc gtaattacat tagttaaaaa
    43441 acttaaaacc attaaataaa taaaaaacca ctaactctat gtgaaataaa tcaaaatttc
    43501 acgccgaaat actccttagg atgtatagcg aaaggagaaa aagatatatc tcgatcaccc
    43561 cctttctccc aagtgaaaat aaaaggttat cagtttgcaa catcgaacaa cattcgttgc
    43621 aaatcgataa caacatgcac cttcaggata ctatttatta tgttcggcaa tgatattttc
    43681 acccgtgtaa aacgttcaga aaataaaaaa atggcggaaa tcgcccaatt cctgcatgaa
    43741 aatgatttga gcgttgacac cacagtcgaa gtatttatta ccgtaacccg cgatgaaaag
    43801 cttatcgcgt gcggtggaat tgccggaaat attattaaat gcgttgctat cagtgaatcc
    43861 gttcgcggtg aaggactggc gctgacatta gccactgaat tgataaacct cgcctatgag
    43921 cggcacagca cgcatctgtt tatttatacc aaaaccgaat acgaggcgct gttccgccag
    43981 tgcggttttt ccacgctgac cagtgtaccc ggcgtgatgg tgctgatgga aaacagcgcc
    44041 acgcgactga aacgctatgc cgaatcgctg aaaaaatttc gtcatccagg gaacaagatt
    44101 ggctgcattg tgatgaacgc caatcccttt acgaatggtc accgttatct gattcaacag
    44161 gctgcagcac agtgcgactg gttgcatctg tttttagtta aagaagattc ttcacgcttt
    44221 ccctatgaag accggctgga tctggtgtta aaaggcaccg ccgatattcc acgcctgact
    44281 gtgcatcgcg gctccgaata catcatctcc cgcgctacgt tcccttgcta cttcattaaa
    44341 gaacagagcg tcattaacca ttgttacacc gaaattgatc tgaagatttt ccgtcagtac
    44401 ctcgctcccg cgctgggtgt aactcaccgc tttgtcggta ctgaaccctt ttgtcgtgtt
    44461 accgcccagt acaaccagga tatgcgctac tggctggaaa cgccgactat ctccgcaccg
    44521 cccatcgaac tggttgaaat tgagcggctg cgttaccagg agatgccgat atccgcttcc
    44581 cgggtacgtc aactgctggc gaaaaacgat ctcacggcta tcgcgccgct ggtccctgca
    44641 gtcacgctgc attatttgca gaacctgctt gagcactccc gccaggacgc ggcagctcgt
    44701 caaaagaccc ccgcatgaga aacaggtgaa aaatgaaaat aaaccagccc gccgttgcag
    44761 gcacccttga gtctggggat gtgatgatac gcatcgcccc actcgatacg caggatatcg
    44821 acctgcaaat caacagcagc gttgagaaac agtttggcga tgcaattcac accaccattc
    44881 tggacgttct cgcccgctac aacgtgcgcg gcgtacagct gaatgtcgat gacaaaggcg
    44941 cactggactg cattttacgt gcacgactgg aagccctgct ggcacgcgcc agcggtatcc
    45001 cggctctgcc atgggaggat tgccaatgat ttccgcttcg ctgcaacaac gtaaaactcg
    45061 cacccgccgc agcatgctgt ttgtgccagg tgccaatgcc gcgatggtca gcaactcatt
    45121 catctacccg gctgatgccc tgatgtttga cctcgaagac tccgtggcat tgcgtgaaaa
    45181 agacaccgcc cgccgcatgg tttaccacgc gctgcaacat ccgctgtatc gcgatattga
    45241 aaccattgtg cgtgtaaacg cgctggattc cgaatggggt gttaacgacc tggaagccgt
    45301 cgttcgcggt ggtgcggacg ttgtgcgtct gccgaaaacc gataccgctc aggatgttct
    45361 ggatatcgaa aaagagatcc tgcgcatcga aaaagcctgt ggtcgtgaac ccggcagcac
    45421 cggcctgctg gcagcgattg aatctccgct gggcattacc cgcgcagtgg aaatcgctca
    45481 cgcttccgag cgtttgatcg gtatcgccct cggtgcagaa gactatgtgc gcaacctgcg
    45541 tacagaacgc tccccggaag gaactgaact gctgttcgca cgctgctcca ttttgcaggc
    45601 cgcgcgctct gcgggtattc aggcgttcga taccgtctat tccgacgcta acaacgaagc
    45661 cggattcctg caagaagccg cccacatcaa acagctgggc tttgacggca aatcgctgat
    45721 caacccgcgt cagattgatc tgctgcacaa cctctacgca ccgactcaga aagaagtgga
    45781 tcacgcccgc cgcgtcgtag aagccgctga agccgccgct cgcgaaggcc tcggcgtggt
    45841 ttccctgaac ggcaagatgg tggacggtcc ggttatcgat cgcgcccgtc tggtgctctc
    45901 ccgtgcagaa ctttccggca tccgcgaaga ataaggcaat caaaatgacg cagaaaattg
    45961 aacaatctca acgacaagaa cgggtagcgg cctggaatcg tcgcgctgaa tgcgatcttg
    46021 ccgctttcca gaactcgcca aagcaaacct accaggctga aaaagcgcgc gatcgcaaac
    46081 tgtgcgccaa cctggaagaa gcgattcgtc gctctggttt acaggacggt atgacggttt
    46141 ccttccatca cgctttccgt ggcggtgacc tgaccgtcaa tatggtgatg gacgtcatcg
    46201 cgaagatggg ctttaaaaac ctgactctgg cctccagctc cctgagtgat tgccatgcgc
    46261 cgctggtaga acacattcgc cggggcgtgg ttacccgcat ttatacctct ggcctgcgcg
    46321 gtccactggc ggaagaaatc tcccgtggtc tgctggcgga accggtgcaa atccactctc
    46381 acggcggacg tgtgcatctg gtacagagcg gcgaactgaa tatcgatgtg gctttcctcg
    46441 gcgtcccgtc ctgtgatgaa ttcggtaatg ccaacggcta ctccggtaaa gcctgctgcg
    46501 gctccctcgg ctatgcaatg gttgatgccg acaacgcaaa acaggtcgtg atgcttaccg
    46561 aagaactgct gccttatccg cataatccgg caagcattga gcaagatcag gttgatctga
    46621 tcgtcaaagt tgatcgcgtt ggcgatgctg caaaaatcgg cgctggtgct acccgtatga
    46681 ccactaaccc gcgcgaactg cttattgccc gtagcgctgc ggatgtgatt gttaactctg
    46741 gctacttcaa agaaggtttc tccatgcaga ccggcaccgg cggcgcttcg ctggcggtaa
    46801 cccgtttcct ggaagacaaa atgcgtagcc gcgatattcg cgccgacttc gccctcggcg
    46861 gtattaccgc aacgatggtt gatctgcacg aaaaaggttt gatccgcaaa ctgctggatg
    46921 tgcagagctt tgacagccac gctgcgcaat cgctggcccg taaccccaat cacatcgaaa
    46981 tcagcgccaa ccagtacgct aactggggtt cgaaaggtgc atcggttgat cgtctcgacg
    47041 tggtggtact gagcgcgctg gaaattgaca cccagttcaa cgttaacgtg ctgaccggct
    47101 ctgacggcgt actgcgtggc gcttccggtg gtcactgcga taccgcgatt gcctctgcac
    47161 tttccatcat cgtcgcaccg ctggtacgcg gtcgtattcc gactctggtg gataacgtgc
    47221 tgacctgcat caccccgggc tccagtgtcg atattctggt cacagaccac ggtatcgcag
    47281 ttaacccggc acgtccggaa ctggcagaac gtctgcagga agcgggcatt aaagtggttt
    47341 ccattgagtg gctgcgtgaa cgtgcgcgtc tgctgaccgg tgaaccacag ccgattgaat
    47401 tcaccgaccg cgtcgttgcc gttgtgcgtt accgcgatgg ctcggtgatc gatgttgtgc
    47461 atcaggtgaa ggaataagcc atgcacctgc ttcctgaact cgccagccac catgcggtgt
    47521 caattcccga gctgctcgtc agccgggatg aaaggcaagc acggcaacac gtctggctca
    47581 agcgccatcc tgttccactg gtctccttta ccgtggttgc gcctgggccg attaaagaca
    47641 gcgaggtcac acgccgaatt tttaatcatg gcgtgacagc cttgcgtgcc ttagccgcaa
    47701 aacagggctg gcaaattcag gagcaggctg cactggtttc cgccagcggg ccggagggca
    47761 tgttgagcat tgccgccccg gctcgcgacc tcaagctcgc caccattgag cttgaacata
    47821 gtcatcctct cgggcggtta tgggatatcg atgtcctgac acccgaaggc gacattctct
    47881 cccgccgcga ttattcactg ccgcctcgcc gctgcctgtt gtgcgagcaa agcgcagctg
    47941 tttgcgcgcg tggaaaaacc catcaactga ccgatttact caaccgcatg gaggcgctgc
    48001 tgaacgatgt cgatgcctgc aacgtcaact aaaaccacaa agcttgcgac gtcattaatc
    48061 gatgagtacg ccctgctggg ctggcgcgcc atgctgactg aagtcaatct gtcaccgaaa
    48121 ccaggcctcg tggatcgcat taactgcggt gcgcacaaag atatggcgct ggaagatttc
    48181 caccgcagcg cgctggcgat tcagggctgg ttaccccgtt tcattgaatt tggtgcctgt
    48241 agtgcagaaa tggcaccgga agcagtactc aacggattac gcccaattgg tatggcttgc
    48301 gaaggtgata tgttccgcgc cactgcgggc gtaaacacgc ataaaggaag cattttttct
    48361 ttagggctac tgtgtgcggc aattggccgt ttgcttcagc tcaaccagcc ggtaacgcca
    48421 acaaccgttt gttcgacggc ggcaagtttc tgtcgtggcc tgaccgatcg cgaactgcgt
    48481 accaataatt cacaactgac ggcaggtcaa cggttgtacc agcagcttgg ccttaccggc
    48541 gcacgcggtg aagccgaagc gggttatcca ctggtgatca accacgcctt gccgcattac
    48601 ctcactctgc tggatcaggg gttagatcct gaactggcat tgctcgatac cttgctccta
    48661 ctgatggcta ctaacggcga taccaacgtt gcatcgcgcg gtggcgaggg gggcctgcgc
    48721 tggctacagc gcgaggcgca aacactatta aacaatgggg gcatccgaac ccccgccgat
    48781 ctcgattatc tccggcagtt cgacagggag tgtatcgaac gaaatctcag tccaggcggc
    48841 agtgccgacc tgctgattat tacctggttt ttagcacaga tttaataatt taagcacttg
    48901 ataaaattgg aatattaatt gtcggagaac ccgtatgtct ttagcaaaag ataatatatg
    48961 gaaattattg gcgcccctgg tggtgatggg tgtcatgttt cttatccctg tacctgacgg
    49021 tatgccgccg caggcgtggc attactttgc agtgtttgtg gcaatgattg tcggcatgat
    49081 cctcgagcca attccggcaa cggcgatcag ttttattgcg gttactattt gcgttattgg
    49141 cagtaattac ctgctctttg atgccaaaga attagctgac ccagcgttta atgcgcaaaa
    49201 acaggcgctg aaatgggggc tggcaggttt ctccagcacc accgtctggc tggtgtttgg
    49261 cgcatttatt tttgcgctgg gctatgaagt caccggtctg ggccgtcgta tcgccctttt
    49321 cctggtgaaa ttcatgggta aacgcacgct gacgctgggt tacgcgattg tcattatcga
    49381 cattctgctg gcaccgttta caccgtccaa caccgcgcgt accgggggta cggtttttcc
    49441 ggtcattaaa aacctgccgc cgctgtttaa atcattcccg aacgatccgt ccgcgcgtcg
    49501 tattggcggc tatttgatgt ggatgatggt cattagtacc agtctgagtt cgtccatgtt
    49561 tgtcaccggt gcggcaccaa acgtgctggg tctggagttc gtcagcaaaa tcgcagggat
    49621 ccagattagc tggctgcaat ggttcctgag cttcctgccg gtcggtatta ttttgctgat
    49681 cgttgctccg tggctctcct atgtgctgta caagccggaa gtgactcaca gtgccgaagt
    49741 ggcagcatgg gccggtgatg aactgaaaac gatgggtgca ttgagccgta aagagtggac
    49801 cctgataggt ctggtgctgc tgagcttagg cttatgggta ttcggcggcg aaatgatcga
    49861 cgccacggcg gtaggtctgc tggcggtttc gctgatgctg gccctgcacg ttgtaccgtg
    49921 gaaagacatt acccgctaca acagcgcctg gaacacactg gtcaacctgg caacgctggt
    49981 tgttatggcg aacggtttga cccgctctgg ttttatcgac tggttcgcta gcaccatgag
    50041 cacgcacctg gaaggcttct caccgaacgc aacggtaatt gtactggttc tggtgttcta
    50101 ctttgcacac tacctgtttg ccagcctgtc tgcgcacacc gcaaccatgc tgccggttat
    50161 tctggccgtc ggtaaaggta ttccgggcgt accaatggaa caactgtgta tcctgctggt
    50221 gctgtctatc ggtatcatgg gctgtctgac gccgtatgca accggtcctg gggtgattat
    50281 ttacggctgt ggctatgtga aatcaaaaga ttactggcgt cttggcgcaa tcttcggggt
    50341 gatttacatc tctatgctgc tgttggttgg ctggccgatt ctcgccatgt ggaactaata
    50401 cgttcgccaa acgctgaata aatcatcacg cccgccaggt aagcccacct ggcgggcttt
    50461 tttatgattt aatagatagt attcagtata tgtatgagtt ccacacccat tatgaaagca
    50521 ttctggcgta acgccgcgtt gctcgcggtt tctctgcttc ccttctcttc tgccaacgcc
    50581 gtggcgttgc aggcaaaaca gtatggcgat tttgatcgct atgtcctggc cctctcctgg
    50641 caaaccggat tttgccagag tcaacacgat cgaaatcgta acgaacgaga tgaatgtcgc
    50701 ctgcaaaccg aaacgaccaa caaagctgat tttctgaccg tacatggtct gtggccagga
    50761 ttgcctaaat cggttgctgc ccgtggtgtt gatgaacgcc gctggatgcg cttcggttgc
    50821 gctactcgcc caatcccgaa tctaccagaa gcgcgcgcca gccgaatgtg ttcatctccg
    50881 gaaaccgggt tatcgctgga aacggccgct aaactaagtg aagtcatgcc aggagctggc
    50941 ggacgttcct gcctggaacg ctacgaatat gccaaacacg gtgcctgctt tggttttgat
    51001 ccagacgcat acttcggcac gatggtacgc ctgaatcaag aaattaaaga gaacgaagcc
    51061 ggaaaattcc ttgcggataa ctacggtaaa acagtgagcc gccgtgactt tgatgccgcc
    51121 tttgccaaaa gctggggaaa agagaacgtg aaagcagtta agctaacgtg ccagggtaac
    51181 cctgcgtatt tgactgaaat tcagatctcg atcaaagctg acgccatcaa cgctccgctt
    51241 tctgccaact cattcttgcc acaaccacac ccaggcaact gtggcaaaac ctttgtgatt
    51301 gataaagcag gttattaatc ctctgattta caaaacacgc taattcgtct tccataaacc
    51361 atgacgatta agatttatac ataatgcgct acattgagga attccctgaa tgtgacgcaa
    51421 atcacttcaa agagtgagca acagtcagta tcatcatgtg agttaaaccc tcgccgcctg
    51481 acggtgaggg ttttcttttg ggtatgttta tctgcgacac tcgcagtacc gacgacatgg
    51541 ag
//
LOCUS       DAMZFT010000017        48495 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.017, whole genome shotgun sequence.
ACCESSION   DAMZFT010000017 DAMZFT010000000
VERSION     DAMZFT010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 48495)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48495)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48495
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.017"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..73
                     /locus_tag="PN977_001151"
     CDS             <1..73
                     /locus_tag="PN977_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cse2/CasB"
                     /protein_id="HDI6041207.1"
                     /translation="TEFIRIRWALEYYQAGDADNEQN"
     gene            88..1143
                     /gene="cas7e"
                     /locus_tag="PN977_001152"
     CDS             88..1143
                     /gene="cas7e"
                     /locus_tag="PN977_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein
                     Cas7/Cse4/CasC"
                     /protein_id="HDI6041208.1"
                     /translation="MTTFIQLHLLTAYPAANLNRDDTGAPKTVVLGGATRLRVSSQSL
                     KRAWRTSALFEQALAGHIGIRSGRIAREAATIMIEKGIEEKKAIEWAADIADYLGKAK
                     KDKKPKDPLTNAETEQLVHISPAEFDAVKALAHQLAEEKRAPKEDDLALLRKDRMAVD
                     IAMFGRMLANKPEFNVEAACQVAHAFGVSETIVEDDFFTAVDDLRQASEDAGAGHLGE
                     TGFGSALFYTYICIDKDLLVENLGGDEALANQTLRAFTEAAFKVSPTGKQNSFASRAY
                     ASWALAEKGTEQPRSLAAAFYEPINGTRQLDVAVQRITTLRENMNTVYEQKTECASFD
                     VMNKQGSMKDVLDFICA"
     gene            1154..1900
                     /gene="cas5e"
                     /locus_tag="PN977_001153"
     CDS             1154..1900
                     /gene="cas5e"
                     /locus_tag="PN977_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cas5/CasD"
                     /protein_id="HDI6041209.1"
                     /translation="MSQYLIFQLHGPMASWGVDAPGEVRHSHELPSRSALLGLLAAGV
                     GIRRDDTECLNAFNRHYSLVVCASRNPRWARDYHTVQMPKEVRKARYFSRREELSDPD
                     LLSAIISRRDYYTDAWWMVAVATTADAPYSLEQLQEGLRHPVFPLYLGRKSHPLALPL
                     APLLLEGNASDALRNAYQQYQDRFHELKVSLPKLQDECWWEGEHDGLVSSKILRRRDV
                     PLNRQQWLFGERTVNQGPWLSKEEPCTSQE"
     gene            1882..2532
                     /gene="cas6e"
                     /locus_tag="PN977_001154"
     CDS             1882..2532
                     /gene="cas6e"
                     /locus_tag="PN977_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000281458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein
                     Cas6/Cse3/CasE"
                     /protein_id="HDI6041210.1"
                     /translation="MYLSRITLHTGQLSPAQLLHLVDRGEYVMHQWLWDLFPGGKERQ
                     FLYRREELQGAFRFFVLSQERPAESETFTIECRSFAPELHTGQSLCFNLRANPTVCKA
                     GKRHDLLMEAKRQVRGQAEGRNVWLHQQQAALDWLAAQGERSGFTLLDTSVDAYRQQQ
                     LRRENSRQLIQFSSVDYTGMLTVTDPGLFLQRLCLGYGKSRAFGCGLMLIKPGAEA"
     gene            2529..3452
                     /gene="cas1e"
                     /locus_tag="PN977_001155"
     CDS             2529..3452
                     /gene="cas1e"
                     /locus_tag="PN977_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated endonuclease Cas1"
                     /protein_id="HDI6041211.1"
                     /translation="MTFVPLSPIPLKDRTSMIFLQYGQVDVLDGAFVLIDKTGIRTHI
                     PVGSVACIMLEPGTRVSHAAVHLAATVGTLLVWVGEAGVRVYSSGQPGGARADKLLYQ
                     AKLALTEDLRLKVVRKMYELRFREPPPARRSVEQLRGIEGSRVRQTYALLAKQYGVKW
                     NGRKYDPKDWEKGDVVNRCISAATSCLYGISEAAVLAAGYAPAIGFIHSGKPLSFVYD
                     IADIIKFDLVVPKAFEIAARHPAEPDKEVRLACRDIFRSSKLTGKLIPLIEEVLAAGE
                     IEPPQPAPDMLPPAIPEPELLGDSGHRGRSG"
     gene            3449..3742
                     /gene="cas2"
                     /locus_tag="PN977_001156"
     CDS             3449..3742
                     /gene="cas2"
                     /locus_tag="PN977_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated endoribonuclease
                     Cas2"
                     /protein_id="HDI6041212.1"
                     /translation="MSMVVVVTENVPPRLRGRLAIWLLEVRAGVYVGDTSKRIREMIW
                     QQISQLAGCGNVVMAWATNTESGFEFQTWGENRRIPVDLDGLRLVSFLPVENQ"
     repeat_region   3839..4965
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=3839..3867
                     /rpt_unit_seq="gtgttccctgcgccagcggggataaaccg"
     gene            complement(5049..6086)
                     /gene="iap"
                     /locus_tag="PN977_001157"
     CDS             complement(5049..6086)
                     /gene="iap"
                     /locus_tag="PN977_001157"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase isozyme conversion
                     aminopeptidase"
                     /protein_id="HDI6041213.1"
                     /translation="MFSALRHRTAALALGVCFILPVHASSPKPGDFANTQARHIATFF
                     PGRMTGTPAEMLSADYIRQQFQQMGYRSDIRTFNSRYIYTARDNRKSWHNVTGSTVIA
                     AHEGKAPQQIIIMAHLDTYAPLSDADADANLGGLTLQGMDDNAAGLGVMLELAERLKN
                     TPTEYGIRFVATSGEEEGKLGAENLLKRMSDSEKKNTLLVINLDNLIVGDKLYFNSGV
                     KTPEAVRKLTRDRALAIARSHGIAATTNPGLNKNYPKGTGCCNDAEVFDKAGIAVLSV
                     EATNWNLGNKDGYQQRAKTAAFPAGNSWHDVRLDNQQHIDKALPGRIERRCRDVMRIM
                     LPLVKELAKAS"
     gene            6338..7246
                     /gene="cysD"
                     /locus_tag="PN977_001158"
     CDS             6338..7246
                     /gene="cysD"
                     /locus_tag="PN977_001158"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HDI6041214.1"
                     /translation="MDQIRLTHLRQLEAESIHIIREVAAEFSNPVMLYSIGKDSSVML
                     HLARKAFYPGTLPFPLLHVDTGWKFREMYEFRDRTAKAYGCELLVHKNPEGVAMGINP
                     FVHGSAKHTDIMKTEGLKQALNKYGFDAAFGGARRDEEKSRAKERIYSFRDRFHRWDP
                     KNQRPELWHNYNGQINKGESIRVFPLSNWTEQDIWQYIWLENIDIVPLYLAAERPVLE
                     RDGMLMMIDDNRIDLQPGEVIKKRMVRFRTLGCWPLTGAVESNAQTLPEIIEEMLVST
                     TSERQGRVIDRDQAGSMELKKRQGYF"
     gene            7248..8675
                     /gene="cysN"
                     /locus_tag="PN977_001159"
     CDS             7248..8675
                     /gene="cysN"
                     /locus_tag="PN977_001159"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysN"
                     /protein_id="HDI6041215.1"
                     /translation="MNTALAQQIANEGGVEAWMIAQQHKSLLRFLTCGSVDDGKSTLI
                     GRLLHDTRQIYEDQLSSLHNDSKRHGTQGEKLDLALLVDGLQAEREQGITIDVAYRYF
                     STEKRKFIIADTPGHEQYTRNMATGASTCELAILLIDARKGVLDQTRRHSFISTLLGI
                     KHLVVAINKMDLVDYSEETFTRIREDYLTFAGQLPGNLDIRFVPLSALEGDNVASQSE
                     SMPWYSGPTLLEVLETVEIQRVVDAQPMRFPVQYVNRPNLDFRGYAGTLASGRVEVGQ
                     RVKVLPSGVESNVARIVTFDGDREEAFAGEAITLVLTDEIDISRGDLLLAADEALPAV
                     QSASVDVVWMAEQPLSPGQSYDIKIAGKKTRARVDGIRYQVDINNLTQREVENLPLNG
                     IGLVDLTFDEPLVLDRYQQNPVTGGLIFIDRLSNVTVGAGMVHEPVSQATAAPSEFSA
                     FELELNALVRRHFPHWGARDLLGDK"
     gene            8675..9280
                     /gene="cysC"
                     /locus_tag="PN977_001160"
     CDS             8675..9280
                     /gene="cysC"
                     /locus_tag="PN977_001160"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HDI6041216.1"
                     /translation="MALHDENVVWHSHPVTVQQRELHHGHRGVVLWFTGLSGSGKSTV
                     AGALEEALHKLGVSTYLLDGDNVRHGLCSDLGFSDADRKENIRRVGEVANLMVEAGLV
                     VLTAFISPHRAERQMVRERVGEGRFIEVFVDTPLAICEARDPKGLYKKARAGELRNFT
                     GIDSVYEAPESAEIHLNGEQLVTNLVQQLLDLLRQNDIIRS"
     gene            9330..9653
                     /locus_tag="PN977_001161"
     CDS             9330..9653
                     /locus_tag="PN977_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3561 family protein"
                     /protein_id="HDI6041217.1"
                     /translation="MRNSHNITLTNNDSLTEDEETTWSLPGAVVGFISWLFALAMPML
                     IYGSNTLFFFIYTWPFFLALMPVAVVVGIALHSLMDGKLRYSIVFTLVTVGIMFGALF
                     MWLLG"
     gene            9847..10158
                     /gene="ftsB"
                     /locus_tag="PN977_001162"
     CDS             9847..10158
                     /gene="ftsB"
                     /locus_tag="PN977_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsB"
                     /protein_id="HDI6041218.1"
                     /translation="MGKLTLLLLAILVWLQYSLWFGKNGIHDYTRVNDDVAAQQATNA
                     KLKARNDQLFAEIDDLNGGQEALEERARNELSMTRPGETFYRLVPDASKRAQSAGQNN
                     R"
     gene            10177..10887
                     /gene="ispD"
                     /locus_tag="PN977_001163"
     CDS             10177..10887
                     /gene="ispD"
                     /locus_tag="PN977_001163"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HDI6041219.1"
                     /translation="MATTHLDVCAVVPAAGFGRRMQTECPKQYLSIGNQTILEHSVHA
                     LLAHPRVTRVVIAISPGDSRFAKLPLANHPQITVVDGGDERADSVLAGLKAAGDAQWV
                     LVHDAARPCLHQDDLARLLALSETSRTGGILAAPVRDTMKRAEPGKNAIAHTVDRNGL
                     WHALTPQFFPRELLHDCLTRALNEGATITDEASALEYCGFHPQLVEGRADNIKVTRPE
                     DLALAEFYLTRTIHQENT"
     gene            10887..11366
                     /gene="ispF"
                     /locus_tag="PN977_001164"
     CDS             10887..11366
                     /gene="ispF"
                     /locus_tag="PN977_001164"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HDI6041220.1"
                     /translation="MRIGHGFDVHAFGGEGPIIIGGVRIPYEKGLLAHSDGDVALHAL
                     TDALLGAAALGDIGKLFPDTDPAFKGADSRELLREAWRRIQAKGYALGNVDVTIIAQA
                     PKMLPHIPQMRVFIAEDLGCHMDDVNVKATTTEKLGFTGRGEGIACEAVALLIKATK"
     gene            11363..12412
                     /gene="truD"
                     /locus_tag="PN977_001165"
     CDS             11363..12412
                     /gene="truD"
                     /locus_tag="PN977_001165"
                     /EC_number="5.4.99.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(13) synthase TruD"
                     /protein_id="HDI6041221.1"
                     /translation="MIEFDNLTYLHGKPQGTGLLKANPEDFVVVEDLGFEPDGEGEHI
                     LVRILKNGCNTRFVADALAKFLKIHAREVSFAGQKDKHAVTEQWLCARVPGKEMPDLS
                     AFQLEGCQVLEYARHKRKLRLGALKGNAFTLVLREVSNRDDVEQRLIDICVKGVPNYF
                     GAQRFGIGGSNLQGALRWAQTNTPVRDRNKRSFWLSAARSALFNQIVAERLKKADVNQ
                     VVDGDALQLAGRGSWFVATTEEVAELQRRVNDKELMITAALPGSGEWGTQREALAFEQ
                     AAVAAETELQALLVREKVEAARRAMLLYPQQLSWNWWDDVTVEIRFWLPAGSFATSVV
                     RELINTTGDYAHIAE"
     gene            12393..13154
                     /gene="surE"
                     /locus_tag="PN977_001166"
     CDS             12393..13154
                     /gene="surE"
                     /locus_tag="PN977_001166"
                     /EC_number="3.1.3.5"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HDI6041222.1"
                     /translation="MRILLSNDDGVHAPGIQTLAKALREFADVQVVAPDRNRSGASNS
                     LTLESSLRTFTFENGDIAVQMGTPTDCVYLGVNALMRPRPDIVVSGINAGPNLGDDVI
                     YSGTVAAAMEGRHLGFPALAVSLDGHKHYDTAAAVTCSILRALCKEPLRTGRILNINV
                     PDLPLDQIKGIRVTRCGTRHPADQVIPQQDPRGNTLYWIGPPGGKCDAGPGTDFAAVD
                     EGYVSITPLHVDLTAHSAQDVVSDWLNSVGVGTQW"
     gene            13148..13774
                     /gene="pcm"
                     /locus_tag="PN977_001167"
     CDS             13148..13774
                     /gene="pcm"
                     /locus_tag="PN977_001167"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HDI6041223.1"
                     /translation="MVSRRVQALLDQLRAQGIQDEQVLNALAAVPREKFVDEAFEQKA
                     WDNIALPIGQGQTISQPYMVARMTELLELTPQSRVLEIGTGSGYQTAILAHLVQHVCS
                     VERIKGLQWQARRRLKNLDLHNVSTRHGDGWQGWQARAPFDAIIVTAAPPEIPTALMT
                     QLDEGGILVLPVGEEHQYLKRVRRRGGEFIIDTVEAVRFVPLVKGELA"
     gene            13914..15053
                     /gene="nlpD"
                     /locus_tag="PN977_001168"
     CDS             13914..15053
                     /gene="nlpD"
                     /locus_tag="PN977_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator NlpD"
                     /protein_id="HDI6041224.1"
                     /translation="MSAGSPKFTVRRIAALSLVSLWLAGCSDTSNPPAPVSSVNGNAP
                     ANTNSGMLITPPPKMGTTSTAQQPQIQPVQQPQIQATQQPQIQPVQPVAQQPVQMENG
                     RIVYNRQYGNIPKGSYSGSTYTVKKGDTLFYIAWITGNDFRDLAQRNNIQAPYALNVG
                     QTLQVGNASGTPITGGNAITQADAAEQGVVIKPAQNSTVAVASQPTITYSESSGEQSA
                     NKMLPNNKPTATTVTAPVTVPTASTTEPTVSSTSTSTPISTWRWPTEGKVIETFGASE
                     GGNKGIDIAGSKGQAIIATADGRVVYAGNALRGYGNLIIIKHNDDYLSAYAHNDTMLV
                     REQQEVKAGQKIATMGSTGTSSTRLHFEIRYKGKSVNPLRYLPQR"
     gene            15116..16108
                     /gene="rpoS"
                     /locus_tag="PN977_001169"
     CDS             15116..16108
                     /gene="rpoS"
                     /locus_tag="PN977_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000081498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoS"
                     /protein_id="HDI6041225.1"
                     /translation="MSQNTLKVHDLNEDAEFDENGVEVFDEKALVEEEPSDNDLAEEE
                     LLSQGATQRVLDATQLYLGEIGYSPLLTAEEEVYFARRALRGDVASRRRMIESNLRLV
                     VKIARRYGNRGLALLDLIEEGNLGLIRAVEKFDPERGFRFSTYATWWIRQTIERAIMN
                     QTRTIRLPIHIVKELNVYLRTARELSHKLDHEPSAEEIAEQLDKPVDDVSRMLRLNER
                     ITSVDTPLGGDSEKALLDILADEKENGPEDTTQDDDMKQSIVKWLFELNAKQREVLAR
                     RFGLLGYEAATLEDVGREIGLTRERVRQIQVEGLRRLREILQTQGLNIEALFRE"
     gene            complement(16228..16635)
                     /locus_tag="PN977_001170"
     CDS             complement(16228..16635)
                     /locus_tag="PN977_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001208086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDI6041226.1"
                     /translation="MALRNKAFHQLRQLFQQHTARWQHELPDLTKPQYAVMRAIADKP
                     GIEQVALIEAAVSTKATLAEMLARMENRGLVRREHDSADKRRRFVWLTAEGEKVLAAA
                     IPIGDSVDEEFLGRLSAEEQELFMQLVRKMMSK"
     gene            16782..17375
                     /locus_tag="PN977_001171"
     CDS             16782..17375
                     /locus_tag="PN977_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HDI6041227.1"
                     /translation="MKLIVGMTGATGAPLGVALLQALREMPNVETHLVMSKWARTTIE
                     LETPYSARDVAALADFSHNPADQAATISSGSFRTDGMIVIPCSMKTLAGIRAGYADGL
                     VGRAADVVLKEGRKLVLVPREMPLSTIHLENMLALSRMGVAMVPPMPAFYNHPETVDD
                     IVHHVVARVLDQFGLEHPHARRWQGLPQARNFSQENE"
     gene            17375..18802
                     /locus_tag="PN977_001172"
     CDS             17375..18802
                     /locus_tag="PN977_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HDI6041228.1"
                     /translation="MAFDDLRSFLQALDDHGQLLKISEEVNAEPDLAAAANATGRIGD
                     GAPALWFDNIRGFTDARVAMNTIGSWQNHAISLGLPPNTPVKKQIDEFIRRWDNFPIA
                     PERRANPAWAQNTVDGEKINLFDILPLFRLNDGDGGFYLDKACVVSRDPLDPDNFGKQ
                     NVGIYRMEVKGKRKLGLQPVPMHDIALHLHKAEERGEDLPIAITLGNDPIITLMGATP
                     LKYDQSEYEMAGALRESPYPIATAPLTGFDVPWGSEVILEGVIESRKREIEGPFGEFT
                     GHYSGGRNMTVVRIDNVSYRSKPIFESLYLGMPWTEIDYLMGPATCVPLYQQLKAEFP
                     EVQAVNAMYTHGLLAIISTKKRYGGFARAVGLRAMTTPHGLGYVKMVIMVDEDVDPFN
                     LPQVMWALSSKVNPAGDLVQLPNMSVLELDPGSSPAGITDKLIIDATTPVAPDNRGHY
                     SQPVVDLPETKAWAEKLTAMLAARQ"
     gene            18813..19049
                     /locus_tag="PN977_001173"
     CDS             18813..19049
                     /locus_tag="PN977_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000562981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6041229.1"
                     /translation="MICPRCADEQIEVMAKSPVKDVWTVYQCQHCLYTWRDTEPLRRT
                     SREHYPEAFRMTQKDIDDAPMVPSIPPLLAEGKC"
     gene            complement(19091..19747)
                     /gene="pphB"
                     /locus_tag="PN977_001174"
     CDS             complement(19091..19747)
                     /gene="pphB"
                     /locus_tag="PN977_001174"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase"
                     /protein_id="HDI6041230.1"
                     /translation="MPSTRYQKINVHHYRHIWAVGDIHGDYQLLQSRLHQLSFCPETD
                     LLISVGDNIDRGPESLNVLRLLNQPWFTSVKGNHEAMALDAFATGDGNMWLASGGDWF
                     FELSDTEQKEAIDLLLRFHNLPHIIEITNDRIKYVIAHADYPGDEYQFGKEIAESELL
                     WPVDRVQKSFKGELKEINGADCFIFGHMMFDNIQTFANQVYIDTGSPESRRLSFYKIK
                     "
     gene            complement(19853..22414)
                     /gene="mutS"
                     /locus_tag="PN977_001175"
     CDS             complement(19853..22414)
                     /gene="mutS"
                     /locus_tag="PN977_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HDI6041231.1"
                     /translation="MSAIENFDAHTPMMQQYLKLKAQHPEILLFYRMGDFYELFYDDA
                     KRASQLLDISLTKRGASAGEPIPMAGIPYHAVENYLAKLVNQGESVAICEQIGDPATS
                     KGPVERKVVRIVTPGTISDEALLQERQDNLLAAIWQDSKGFGYATLDISSGRFRLSEP
                     ADRETMAAELQRTNPAELLYAEDFAEMSLIEGRRGLRRRPLWEFEIDTARQQLNLQFG
                     TRDLVGFGVENAPRGLCAAGCLLQYAKDTQRTTLPHIRSITMEREQDSIIMDAATRRN
                     LEITQNLAGGAENTLASVLDCTVTPMGSRMLKRWLHMPVRDTRVLLERQQTIGALQDF
                     TAELQPVLRQVGDLERILARLALRTARPRDLARMRHAFQQLPELRALLENVDSAPVQA
                     LREKMGEFAELRDLLERAIIDTPPVLVRDGGVIAPGYNEELDEWRALADGATDYLERL
                     EVRERERTGLDTLKVGFNAVHGYYIQISRGQSHLAPINYMRRQTLKNAERYIIPELKE
                     YEDKVLTSKGKALALEKQLYEELFDLLLPHLEALQQSASALAELDVLVNLAERAYTLN
                     YTCPTFIDKPGIRITEGRHPVVEQVLNEPFIANPLNLSPQRRMLIITGPNMGGKSTYM
                     RQTALIALMAYIGSYVPAQKVEIGPIDRIFTRVGAADDLASGRSTFMVEMTETANILH
                     NATEYSLVLMDEIGRGTSTYDGLSLAWACAENLANKIKALTLFATHYFELTQLPEKME
                     GVANVHLDALEHGDTIAFMHSVQDGAASKSYGLAVAALAGVPKEVIKRARQKLRELES
                     ISPNAAATQVDGTQMSLLSVPEETSPAVEALENLDPDSLTPRQALEWIYRLKSLV"
     gene            22490..22624
                     /locus_tag="PN977_001176"
     CDS             22490..22624
                     /locus_tag="PN977_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6041232.1"
                     /translation="MIPELIISGEEANGEFLMVIPGKMLFCSFCVMLMKQVRKIASVE
                     "
     gene            22701..23045
                     /locus_tag="PN977_001177"
     CDS             22701..23045
                     /locus_tag="PN977_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide-stimulated promoter family
                     protein"
                     /protein_id="HDI6041233.1"
                     /translation="MSGKRISREKLTIKKMIDLYQAKCPQASAEPEHYDALFAYAQKR
                     LDKCVFGEEKPACKQCPVHCYQPAKREEMKQIMRWAGPRMLWRHPILTVRHLIDDKRP
                     VPELPEKYRPKK"
     gene            complement(23084..23512)
                     /locus_tag="PN977_001178"
     CDS             complement(23084..23512)
                     /locus_tag="PN977_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012134844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TOBE domain-containing protein"
                     /protein_id="HDI6041234.1"
                     /translation="MAVSARNQLTGTVSAVAMGAVNDEVELTLAGGAKLVAIVTHSSQ
                     QALGLAKGKEAIALIKAPWVTLATEDCGLKFSARNQFAGSVSTITEGAVNATVHIKTD
                     AGFEIVAVVTNESQDEMKLTTGSRVIALIKASAILIATKA"
     gene            complement(23557..25635)
                     /gene="flhA"
                     /locus_tag="PN977_001179"
     CDS             complement(23557..25635)
                     /gene="flhA"
                     /locus_tag="PN977_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase transcriptional activator
                     FlhA"
                     /protein_id="HDI6041235.1"
                     /translation="MSYTPMSDLGQQGLFDITRTLLQQPDLASLCEALSQLVKRSALA
                     DNAAIVLWQAQTQRASYYASREKDTPIKYEDETVLAHGPVRSILSRPDTLHCSYEEFC
                     ETWPQLAAGGLYPKFGHYCLMPLAAEGHIFGGCEFIRYDDRPWSEKEFNRLQTFTQIV
                     SVVTEQIQSRVVNNVDYELLCRERDNFRILVAITNAVLSRLDMDELVSEVAKEIHYYF
                     DIDDISIVLRSHRKNKLNIYSTHYLDKQHPAHEQSEVDEAGTLTERVFKSKEMLLINL
                     HERDDLAPYERMLFDTWGNQIQTLCLLPLMSGDTMLGVLKLAQCEEKVFTTTNLNLLR
                     QIAERVAIAVDNALAYQEIHRLKERLVDENLALTEQLNNVDSEFGEIIGRSEAMYSVL
                     KQVEMVAQSDSTVLILGETGTGKELIARAIHNLSGRNNRRMVKMNCAAMPAGLLESDL
                     FGHERGAFTGASAQRIGRFELADKSSLFLDEVGDMPLELQPKLLRVLQEQEFERLGSN
                     KIIQTDVRLIAATNRDLKKMVADREFRSDLYYRLNVFPIHLPPLRERPEDIPLLAKAF
                     TFKIARRLGRNIDSIPAETLRILSNMEWPGNVRELENVIERAVLLTRGNVLQLSLPDI
                     ALPEPETPPAATVVAQEGEDEYQLIVRVLKETNGVVAGPKGAAQRLGLKRTTLLSRMK
                     RLGIDKSALI"
     gene            complement(25709..26719)
                     /gene="hypE"
                     /locus_tag="PN977_001180"
     CDS             complement(25709..26719)
                     /gene="hypE"
                     /locus_tag="PN977_001180"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417210.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation carbamoyl dehydratase
                     HypE"
                     /protein_id="HDI6041236.1"
                     /translation="MNNIQLAHGSGGQAMQQLINSLFMEAFANPWLAEQEDQARLDLA
                     QLVAEGDRLAFSTDSYVIDPLFFPGGNIGKLAICGTANDVAVSGAIPRYLSCGFILEE
                     GLPMETLKAVVTSMAETARAAGIAIVTGDTKVVQRGAADKLFINTAGMGAIPANIHWG
                     AQTLTAGDVLLVSGTLGDHGATILNLREQLGLDGELVSDCAVLTPLIQTLRDIPGVKA
                     LRDATRGGVNAVVHEFAAACGCGIELSEAALPVKPAVRGVCELLGLDALNFANEGKLV
                     IAVERNAAEQVLAALHSHPLGKDAALIGEVVERKGVRLAGLYGVKRTLDLPHAEPLPR
                     IC"
     gene            complement(26716..27837)
                     /gene="hypD"
                     /locus_tag="PN977_001181"
     CDS             complement(26716..27837)
                     /gene="hypD"
                     /locus_tag="PN977_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HDI6041237.1"
                     /translation="MRFVDEYRAPEQVMQLIEHLRERASHLSYTAERPLRIMEVCGGH
                     THAIFKFGLDQLLPENVEFIHGPGCPVCVLPMGRIDTCVEIASHPEVIFCTFGDAMRV
                     PGKQGSLLQAKARGADVRIVYSPMDALKLAQENPTRKVVFFGLGFETTMPTTAITLQQ
                     AKARDVQNFYFFCQHITLIPTLRSLLEQPDNGIDAFLAPGHVSMVIGTDAYNFIASDF
                     HRPLVVAGFEPLDLLQGVVMLVEQKIAAHSKVENQYRRVVPDAGNLLAQQAIADVFCV
                     NGDSEWRGLGVIESSGVHLTPDYQRFDAEAHFRPAPQQVCDDPRARCGEVLTGKCKPH
                     QCPLFGNTCNPQTAFGALMVSSEGACAAWYQYRQQESEA"
     gene            complement(27837..28109)
                     /gene="hypC"
                     /locus_tag="PN977_001182"
     CDS             complement(27837..28109)
                     /gene="hypC"
                     /locus_tag="PN977_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 3 maturation protein HypC"
                     /protein_id="HDI6041238.1"
                     /translation="MCIGVPGQIRTIDGNQAKVDVCGIQRDVDLTLVGSCDENGQPRV
                     GQWVLVHVGFAMSVINEAEARDTLDALQNMFDVEPDVGALLYGEEK"
     gene            complement(28100..28972)
                     /gene="hypB"
                     /locus_tag="PN977_001183"
     CDS             complement(28100..28972)
                     /gene="hypB"
                     /locus_tag="PN977_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HDI6041239.1"
                     /translation="MCTTCGCGEGNLYIEGDEHNPHSAFRSAPFAPAARPKMKITGIK
                     APEFTPSQTEEGDLHYGHGEAGTHAPGMSQRRMLEVEIDVLDKNNRLAERNRARFAAR
                     KQLVLNLVSSPGSGKTTLLTETLMRLKDSVPCAVIEGDQQTVNDAARIRATGTPAIQV
                     NTGKGCHLDAQMIADAAPRLPLDDNGILFIENVGNLVCPASFDLGEKHKVAVLSVTEG
                     EDKPLKYPHMFAAASLMLLNKVDLLPYLNFDVEKCIACAREVNPEIEIILISATSGEG
                     MDQWLNWLETQRCA"
     gene            complement(28976..29326)
                     /gene="hypA"
                     /locus_tag="PN977_001184"
     CDS             complement(28976..29326)
                     /gene="hypA"
                     /locus_tag="PN977_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HDI6041240.1"
                     /translation="MHEITLCQRALELIEQQAAKHGAKRVTGVWLKIGAFSCVETSSL
                     AFCFDLVCRGSMAEGCKLHLEEQEAECWCETCQQYVTLLTQRVRRCPQCHGDMLQIVA
                     DDGLQIRRIEIDQE"
     gene            29538..29999
                     /gene="hycA"
                     /locus_tag="PN977_001185"
     CDS             29538..29999
                     /gene="hycA"
                     /locus_tag="PN977_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase regulator HycA"
                     /protein_id="HDI6041241.1"
                     /translation="MTIWEISEKADYIAQRHRRLQDQWHIYCNSLVQGITLSKARLHH
                     AMSCAPDKELCFVLFEHFRIYVTLADGFNSHTIEYYVETKEGEDKQWIAQAQLSIDGM
                     IDGKVNIRDREQVLEHYLEKIAGVYDSLYTAIENNVPVNLSQLVKGQSPAA"
     gene            30124..30735
                     /gene="hycB"
                     /locus_tag="PN977_001186"
     CDS             30124..30735
                     /gene="hycB"
                     /locus_tag="PN977_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycB"
                     /protein_id="HDI6041242.1"
                     /translation="MNRFVIADSTLCIGCHTCEAACSETHRQHGLQSMPRLRVMLNEK
                     ESAPQLCHHCEDAPCAVVCPVNAITRVDGAVQLNESLCVSCKLCGIACPFGAIEFSGS
                     RPLDIPANANTPKAPPAPPAPARVSTLLDWVPGIRAIAVKCDLCSFDEQGPACVRMCP
                     TKALHLVDNTDIARVSKRKRELTFNTDFGDLTLFQQAQSGEAK"
     gene            30732..32558
                     /gene="hycC"
                     /locus_tag="PN977_001187"
     CDS             30732..32558
                     /gene="hycC"
                     /locus_tag="PN977_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit 3"
                     /protein_id="HDI6041243.1"
                     /translation="MSAISLINSGVAWFVAAAVLAFLFSFQKALSGWIAGIGGAVGSL
                     YTAAAGFTVLTGAVGVSGALSLVSYDVQISPLNAIWLITLGLCGLFVSLYNIDWHRHA
                     QVKCNGLQINMLMAAAVCAVIASNLGMFVVMAEVMALCAVFLTSNSKEGKLWFALGRL
                     GTLLLAIACWLLWQRYGTLDLRLLDMRMQQLPLGSDIWLLGVIGFGLLAGIIPLHGWV
                     PQAHANASAPAAALFSTVVMKIGLLGILTLSLLGGNAPLWWGIALLVLGMITAFVGGL
                     YALMEHNIQRLLAYHTLENIGIILLGLGAGVTGIALEQPALIALGLVGGLYHLLNHSL
                     FKSVLFLGAGSVWFRTGHRDIEKLGGIGKKMPVISIAMLVGLMAMAALPPLNGFAGEW
                     VIYQSFFKLSNSGAFVARLLGPLLAVGLAITGALAVMCMAKVYGVTFLGAPRTKEAEN
                     ATCAPLLMSVSVVALAICCVIGGVAAPWLLPMLSAAVPLPLEPANTTVSQPMITLLLI
                     ACPLLPFIIMAICKGDRLPSRSRGAAWVCGYDHEKSMVITAHGFAMPVKQAFAPVLKL
                     RKWLNPVSLVPGWQCEGSALLFRRMALVELAVLVVIIVSRGA"
     gene            32561..33484
                     /gene="hycD"
                     /locus_tag="PN977_001188"
     CDS             32561..33484
                     /gene="hycD"
                     /locus_tag="PN977_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycD"
                     /protein_id="HDI6041244.1"
                     /translation="MSVLYPLIQALVLFAVAPLLSGITRVARARLHNRRGPGVLQEYR
                     DIIKLLGRQSVGPDASGWVFRLTPYVMVGVMLTIATALPVVTVGSPLPQLGDLITLLY
                     LFAIARFFFAISGLDTGSPFTAIGASREAMLGVLVEPMLLLGLWVAAQVAGSTNISNI
                     TDTVYHWPLSQSIPLVLALCACAFATFIEMGKLPFDLAEAEQELQEGPLSEYSGSGFG
                     VMKWGISLKQLVVLQMFVGVFIPWGQMETFTVGGLLLALVIAIVKLVVGVLVIALFEN
                     SMARLRLDITPRITWAGFGFAFLAFVSLLAA"
     gene            33502..35211
                     /gene="hycE"
                     /locus_tag="PN977_001189"
     CDS             33502..35211
                     /gene="hycE"
                     /locus_tag="PN977_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycE"
                     /protein_id="HDI6041245.1"
                     /translation="MSEEKLGQHYLAALNEAFPGVVLDHAWQTKDQLTVTVKVNYLPE
                     VVEFLYYKQGGWLSVLFGNDERKLNGHYAVYYVLSMEKGTKCWITVRVEVDANKPEYP
                     SVTPRVPAAVWGEREVRDMYGLIPVGLPDERRLVLPDDWPDELYPLRKDSMDYRQRPA
                     PTTDAETYEFINELGDKKNNVVPIGPLHVTSDEPGHFRLFVDGENIIDADYRLFYVHR
                     GMEKLAETRMGYNEVTFLSDRVCGICGFAHSTAYTTSVENAMGIQVPERAQMIRAILL
                     EVERLHSHLLNLGLACHFTGFDSGFMQFFRVRETSMKMAEILTGARKTYGLNLIGGIR
                     RDLLKDDMIQTRQLAQQMRREVQELVDVLLSTPNMEQRTVGIGRLDPEIARDFSNVGP
                     MVRASGHARDTRADHPFVGYGLLPMEVHSEQGCDVISRLKVRINEVYTALNMIDYGLD
                     NLPGGPLMVEGFTYIPHRFALGFAEAPRGDDIHWSMTGDNQKLYRWRCRAATYANWPT
                     LRYMLRGNTVSDAPLIIGSLDPCYSCTDRMTVVDVRKKKSKVVPYKELERYSIERKNS
                     PLK"
     gene            35221..35763
                     /gene="hycF"
                     /locus_tag="PN977_001190"
     CDS             35221..35763
                     /gene="hycF"
                     /locus_tag="PN977_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycF"
                     /protein_id="HDI6041246.1"
                     /translation="MFTFIKKVIKTGTATSSYPLEPIAVDKNFRGKPEQNPQQCIGCS
                     ACVNACPSNALTVETDLATGELAWEFNLGRCIFCGRCEEVCPTAAIKLSQEYELAVWK
                     KEDFLQQSRFALCNCRVCNRPFAVQKEIDYAIALLKHNGDSRAENHRESFETCPECKR
                     QKCLVPSDRIELTRHMKEAI"
     gene            35763..36530
                     /gene="hycG"
                     /locus_tag="PN977_001191"
     CDS             35763..36530
                     /gene="hycG"
                     /locus_tag="PN977_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycG"
                     /protein_id="HDI6041247.1"
                     /translation="MSNLLGPRDANGIPVPMTVDESIASMKASLLKKIKRSAYVYRVD
                     CGGCNGCEIEIFATLSPLFDAERFGIKVVPSPRHADILLFTGAVTRAMRSPALRAWQS
                     APDPKICISYGACGNSGGIFHDLYCVWGGTDKIVPVDVYIPGCPPTPAATLYGFAMAL
                     GLLEQKIHARGPGEQDEQPAEILHGDMVQPLRVKVDREARRLAGYRYGRQIADDFLTQ
                     LGQGEEQVARWLEAENDPRLNEIVSHLNHVVEEARIR"
     gene            36527..36937
                     /gene="hycH"
                     /locus_tag="PN977_001192"
     CDS             36527..36937
                     /gene="hycH"
                     /locus_tag="PN977_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase assembly protein HycH"
                     /protein_id="HDI6041248.1"
                     /translation="MSEKVVFSQLSRKFIDENDATPAEAQQVVYYSLAIGHHLGVIDC
                     LEAALTCPWDDYLAWIATLEAGSEARRKMEGVPKYGEIVIDINHVPMLANAFDKARAA
                     QTSQQKEWSTMLLSMLHDIYQENAIYLMVRRLRD"
     gene            36930..37400
                     /gene="hycI"
                     /locus_tag="PN977_001193"
     CDS             36930..37400
                     /gene="hycI"
                     /locus_tag="PN977_001193"
                     /EC_number="3.4.23.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation peptidase HycI"
                     /protein_id="HDI6041249.1"
                     /translation="MTDVLLCVGNSMMGDDGAGPLLAEKCAAAPKGNWVVIDGGSAPE
                     NDIVAIRELRPTRLLIVDATDMGLNPGEIRIIDPDDIAEMFMMTTHNMPLNYLIDQLK
                     EDIGEVIFLGIQPDIVGFYYPMTQPIKDAVETVYQRLEGWEGNGGFAQLAVEEE"
     gene            37443..38198
                     /locus_tag="PN977_001194"
     CDS             37443..38198
                     /locus_tag="PN977_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001330736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6041250.1"
                     /translation="MCAFLLSLVLPAQATSFTEYLPMSDSEYAQKRALKPLLTMPYDA
                     EQNWHFRKVGVAGVTLEKMPNEEDYWQLTAKDRTGKSWKVPVGLLANMAGKGQFYRAD
                     LDRNGIQDLVIWLPSAGNGLAPYAHLILMTFTRDGRPCVFEPWGFYTASKTGVDDLLD
                     LQGNGHTQLLDMQFDSGYWITSLYQVKDAKWQRVHGWFGKLSYPALTRFTYTPNRKLV
                     LKPIAGRDPQTEDLALTQRCLIKGDVLDGVNQL"
     gene            complement(38285..39709)
                     /gene="ascB"
                     /locus_tag="PN977_001195"
     CDS             complement(38285..39709)
                     /gene="ascB"
                     /locus_tag="PN977_001195"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase AscB"
                     /protein_id="HDI6041251.1"
                     /translation="MSVFPEGFLWGGALAANQSEGAFREGGKGLTTVDMIPHGEHRMA
                     VKLGLEKRFQLRDDEFYPSHEATDFYHRYKEDIALMAEMGFKVFRTSIAWSRLFPQGD
                     ELTPNQQGIAFYRAVFEECKKYGIEPLVTLCHFDVPMHLVTEYGSWRNRKLVEFFSRY
                     ARTCFEAFDGLVKYWLTFNEINIMLHSPFSGAGLVFEEGENQDQVKYQAAHHQLVASA
                     LATKIAHEVNPQNQVGCMLAGGNFYPYSCKPEDVWAALEKDRENLFFIDVQARGAYPA
                     YSARVFREKGVTINKAPGDDEILKNTVDFVSFSYYASRCASAEMNANNSSAANVVKSL
                     RNPYLQVSDWGWGIDPLGLRITMNMMYDRYQKPLFLVENGLGAKDEFAANGEINDDYR
                     ISYLREHIRAMGEAIADGIPLMGYTTWGCIDLVSASTGEMSKRYGFVYVDRDDAGNGT
                     LARTRKKSFWWYKKVIASNGEDLE"
     gene            complement(39718..41175)
                     /gene="ascF"
                     /locus_tag="PN977_001196"
     CDS             complement(39718..41175)
                     /gene="ascF"
                     /locus_tag="PN977_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose/arbutin/salicin transporter
                     subunit IIBC"
                     /protein_id="HDI6041252.1"
                     /translation="MAKNYAALARSVIAALGGVDNISAVTHCMTRLRFVIKDDQLIDS
                     PTLKTISGVLGVVRSDNQCQVIIGNTVSQAFQEVVSLLPGDMQPAQPVGKPKLTLRRI
                     GAGILDALIGTMSPLIPAIIGGSMVKLLAMILEMSGVLTKGSPTLTILNVIGDGAFFF
                     LPLMVAASAAIKFKTNMSLAIAIAGVLVHPSFIELMAKAAQGEHVEFALIPVTAVKYT
                     YTVIPALVMTWCLSYIERWVDSITPAVTKNFLKPMLIVLIAAPLAILLIGPIGIWIGS
                     AISALVYTIHGYLGWLSVAIMGALWPLLVMTGMHRVFTPTIIQTIAETGKEGMVMPSE
                     IGANLSLGGSSLAVAWKTKNPELRQTALAAAASAIMAGISEPALYGVAIRLKRPLIAS
                     LISGFICGAVAGMAGLASHSMAAPGLFTSVQFFDPANPMSIVWVFAVMALAVVLSFIL
                     TLLLGFEDIPVEEAAAQARKHQSVQPTVAKEVSLN"
     gene            41435..42445
                     /gene="ascG"
                     /locus_tag="PN977_001197"
     CDS             41435..42445
                     /gene="ascG"
                     /locus_tag="PN977_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417194.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator AscG"
                     /protein_id="HDI6041253.1"
                     /translation="MTTMLEVAKRAGVSKATVSRVLSGNGYVSQETKDRVFQAVEESG
                     YRPNLLARNLSAKSTQTLGLVVTNTLYHGIYFSELLFHAARMAEEKGRQLLLADGKHS
                     AEEERQAIQYLLDLRCDAIMIYPRFLSVDEIDDIIDAHSQPIMVLNRRLRKNSSHSVW
                     CDHKQTSFNAVAELINAGHQEIAFLTGSMDSPTSIERLAGYKDALSQHGIALNEKLIA
                     NGKWTPASGAEGVETLLERGAKFSVLVASNDDMAIGAMKALHERGVAVPEQVSVIGFD
                     DIAIAPYTVPALSSVKIPVTEMIQEIIGRLIFMLDGGDFSPPKTFSGKLIRRGSLIAL
                     SQ"
     gene            42594..43121
                     /gene="hydN"
                     /locus_tag="PN977_001198"
     CDS             42594..43121
                     /gene="hydN"
                     /locus_tag="PN977_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport protein HydN"
                     /protein_id="HDI6041254.1"
                     /translation="MNRFIIADASKCIGCRTCEVACVVSHQENQDCASLTPETFLPRI
                     HVIKGVNISTATVCRQCEDAPCANVCPNGAISRDKGFVHVMQERCIGCKTCVVACPYG
                     AMEVVVRPVIRNSGAGLNVRADKAEANKCDLCNHREDGPACMAACPTHALICVDRNKL
                     EQLSAEKRRRTALMF"
     gene            43274..45514
                     /gene="hypF"
                     /locus_tag="PN977_001199"
     CDS             43274..45514
                     /gene="hypF"
                     /locus_tag="PN977_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001546942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HDI6041255.1"
                     /translation="MAKNISCGVQLRIRGKVQGVGFRPFVWQLAQQLNLHGDVCNDGD
                     GVEVRLLEAPETFLVQLHQHCPPLARIDSVEREPFIWSQLPTEFTIRQSTGGTMNTQI
                     VPDAATCPACLAEMNTPGERRYRYPFINCTHCGPRFTIIRAMPYDRPFTVMAAFPLCP
                     ACDKEYRDPLDRRFHAQPVACPECGPHLEWVSHGEHAEQEAALQAAIAQLKMGNIVAI
                     KGIGGFHLACDARNSNAVATLRARKHRPAKPLAVMLPVADGLPDAARQLLSTPAAPIV
                     LVDKKYIPELCDDIAPGLNEVGVMLPANPLQHLLLQELQCPLVMTSGNLSGKPPAISN
                     EQALADLQGIADGFLIHNRDIVQRMDDSVVRESGEMLRRSRGYVPDALALPPGFKNVP
                     PVLCLGADLKNTFCLVRGEQAVLSQHLGDLSDDGIQMQWREALRLMQNIYDFTPQYVV
                     HDAHPGYVSSQWAREMNLPTQTVLHHHAHAAACLAEHHWPLEGGDVIALTLDGIGMGE
                     NGALWGGECLRVNYRECQHLGGLPAVALAGGDLAAKQPWRNLLAQCLRFVPEWQNYPE
                     TASVQQQNWSVLARAIERGINAPLASSCGRLFDAVAAALGCAPATLSYEGEAACALEA
                     LAASCDGVTHPVTMPLVDNQLDLATFWQQWLNWQAPVNQRAWAFHDALAQGFAALMRE
                     QATMRGITTLVFSGGVIHNRLLRTRLAHYLADFTLLFPQSLPAGDGGLSLGQGVIAAA
                     RILHAA"
     gene            complement(45593..46726)
                     /gene="norW"
                     /locus_tag="PN977_001200"
     CDS             complement(45593..46726)
                     /gene="norW"
                     /locus_tag="PN977_001200"
                     /EC_number="1.18.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavorubredoxin reductase NorW"
                     /protein_id="HDI6041256.1"
                     /translation="MSNSIVIIGSGFAARQLVKNIRKQDASIPLTLIAADSMDEYNKP
                     DLSHVISQGQRADDLTRQTAGEFAEQFNLRLFPHTWVTDIDAEAHVVKSQNNQWQYDK
                     LVLATGASAFVPPVPGRELMLTLNSQQEYRACETQLRDARRVLIVGGGLIGSELAMDF
                     CRAGKAVTLIDNAASILASLMPPEVSSRLQHRLTEMGVHLLLKSQLQGLEKTDSGILA
                     TLECQRCIEVDAVIAATGLRPETALARRAGLTINRGVCVDSYLQTSNADIYALGDCAE
                     INGQVLPFLQPIQLSAMVLAKNLLGNNTPLKLPAMLVKIKTPELPLHLAGETQRQDLR
                     WQINTERQGMVARGVDDADQLRAFVVSEDRMKEAFGLLKTLPM"
     gene            complement(46723..48162)
                     /gene="norV"
                     /locus_tag="PN977_001201"
     CDS             complement(46723..48162)
                     /gene="norV"
                     /locus_tag="PN977_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic nitric oxide reductase
                     flavorubredoxin"
                     /protein_id="HDI6041257.1"
                     /translation="MSIVVKNNIHWVGQRDWEVRDFHGTEYKTLRGSSYNSYLIREEK
                     NVLIDTVDHKFSREFVQNLRNEIDLADIDYIVINHAEEDHAGALTELMAQIPDTPIYC
                     TANAIDSINGHHHHPEWNFNVVKTGDTLDIGNGKQLIFVETPMLHWPDSMMTYLTGDA
                     VLFSNDAFGQHYCDEHLFNDEVDQTELFEQCQRYYANILTPFSRLVTPKITEILGFNL
                     PVDMIATSHGVVWRDNPTQIVELYLKWAADYQEDRITIFYDTMSNNTRMMADAIAQGI
                     AETDPRVAVKIFNVARSDKNEILTNVFRSKGMLVGTSTMNNVMMPKIAGLVEEMTGLR
                     FRNKRASAFGSHGWSGGAVDRLSTRLQDAGFEMSLSLKAKWRPDQDALELCREHGREI
                     ARQWALAPLPQSTVNTVVKEETSATTTADLGPRMQCSVCQWIYDPAKGEPMQDVAPGT
                     PWSEVPDNFLCPECSLGKDVFDELASEAK"
     gene            48349..>48495
                     /locus_tag="PN977_001202"
     CDS             48349..>48495
                     /locus_tag="PN977_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417189.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6041258.1"
                     /translation="MSFSVDVLANIAIELQRGIGHQDRFQRLITTLRQVLECDASALL
                     RYDSR"
BASE COUNT        11043 a        12688 c        13332 g        11432 t
ORIGIN      
        1 aacagaattt atccgtatcc gctgggcgct ggaatattac caggctggcg acgccgataa
       61 cgaacaaaat taataaagga ctatctcatg acgacattta ttcagcttca tttgttaacc
      121 gcttaccctg cagccaacct taaccgtgat gacaccggtg cgccgaaaac cgtggtgctg
      181 ggtggagcaa cgcgtctgcg cgtttcctcg caaagtctga aacgtgcgtg gcgcacttct
      241 gcactttttg aacaggcgct ggcgggccat attggtattc gtagtgggcg tatcgcgcgc
      301 gaggcagcaa ctatcatgat agagaaagga atcgaagaaa aaaaagccat cgaatgggcg
      361 gcagatattg ccgattatct tggtaaagcc aaaaaagaca aaaaaccaaa agatccgctc
      421 actaacgctg aaactgaaca gctcgtccat atcagcccgg cagaatttga cgccgtaaaa
      481 gcgctggccc atcaactggc agaagaaaag cgcgcgccaa aagaggacga tctcgctttg
      541 ttacgtaaag atcgtatggc ggtagatatc gccatgtttg gtcgtatgct ggcgaataaa
      601 cccgagttta atgttgaagc cgcctgtcag gtcgcgcacg cgtttggtgt cagtgaaacg
      661 attgtcgaag atgatttttt caccgccgtt gatgatttgc gccaggcctc tgaagatgct
      721 ggtgcagggc atcttggtga aactggattt ggttctgcgc tgttctacac ctatatctgc
      781 attgataaag atctgctggt cgaaaacctc ggcggtgacg aagcattagc taatcagacc
      841 ctgcgcgcct ttacggaagc agcgtttaaa gtttccccaa ccggtaaaca aaacagcttt
      901 gccagccgtg cctacgcctc ctgggcgctg gcagaaaaag gcaccgaaca accgcgttct
      961 ctggcggcgg ctttctatga acccattaat ggcacacgtc agttagatgt ggcggtgcag
     1021 cgtattacaa cgcttcgcga aaacatgaat acggtttacg aacagaagac cgaatgcgca
     1081 agttttgacg tgatgaacaa acagggaagc atgaaggacg tgctggactt tatctgcgcg
     1141 taaggggcgg cttatgagcc aatatctgat ttttcagctt catgggccaa tggcatcctg
     1201 gggcgttgat gcacccggtg aggtgcgtca tagccatgaa ctgccttcgc gttcagcatt
     1261 gctggggctg ctggctgccg gggtagggat acggcgtgat gacaccgaat gcttaaacgc
     1321 gtttaatcgc cactattcac tggtggtttg cgccagccgt aacccgcgct gggcacggga
     1381 ttatcacacg gtccagatgc caaaagaggt gcgtaaagcg cgttatttca gccgtcgcga
     1441 agagttgagc gaccctgatc ttctaagcgc gattatctcc cggcgcgact actacaccga
     1501 tgcctggtgg atggtggccg tggcaacaac agctgatgcg ccttacagcc tcgaacagtt
     1561 gcaggaaggt ttacgccatc cggtttttcc gctttatctg gggcgaaaaa gccatccgct
     1621 ggcattaccc cttgcgccgt tactgctcga aggcaacgcg tctgatgcct tacgtaacgc
     1681 ataccaacag tatcaggatc gtttccacga actaaaagtc tcactcccga aacttcagga
     1741 tgaatgctgg tgggaagggg agcacgatgg cctggtctcg agcaaaatat tacgccgccg
     1801 ggatgttcct ttaaatcgtc agcagtggct gtttggggaa cgcaccgtca atcaggggcc
     1861 gtggctcagc aaggaggaac catgtacctc tcaagaataa ccttacatac cggccagctt
     1921 tcgcctgcgc agttgctgca tctggtggat cgcggggaat atgtgatgca tcagtggctg
     1981 tgggatcttt ttcctggcgg caaagaaagg caatttcttt atcgtcggga agaactccag
     2041 ggcgcgtttc gcttttttgt cctgtcgcag gaacgcccgg cggaaagtga aacgttcacc
     2101 atcgaatgcc ggtcatttgc gcctgaactg cataccggac aatccctttg tttcaacctg
     2161 cgggcgaatc caacagtctg taaagcgggt aagcgccacg atctgctgat ggaggcgaaa
     2221 cggcaggtga gggggcaggc ggaaggaaga aatgtctggt tgcatcaaca acaggcggcg
     2281 ctggactggc tggcagcgca gggggagaga agcggtttta cactgctgga tacctcggtc
     2341 gatgcctacc gacaacagca actgcggcgg gaaaactccc ggcaactgat ccagttcagt
     2401 agcgtggatt atacagggat gcttacagtc accgatccag ggttatttct gcaacgtctc
     2461 tgcctggggt atggcaaaag ccgggcgttc ggctgtggtc tgatgttaat caaacccgga
     2521 gcagaggcgt gacgtttgta ccactgagtc cgatcccgtt aaaagatcgc acatctatga
     2581 tcttcctcca gtacggtcag gtcgacgtac tggacggcgc tttcgtgctg atcgacaaaa
     2641 ccgggatccg cacgcatatt ccagtgggat cggtcgcctg cattatgctc gaaccgggaa
     2701 caagagtttc ccacgcggca gtccatctgg ccgccacggt gggaacactg ctggtctggg
     2761 tcggtgaagc gggcgttcgc gtttactcct ccggacaacc cggaggggcg cgggcagata
     2821 aattactcta ccaggcaaag ctggctttaa cggaagatct acggttgaag gtggtgcgca
     2881 aaatgtatga attacgtttt cgtgaaccac cgccagctcg ccgttcagtg gagcagttac
     2941 ggggaattga gggatcccgt gttcgccaga cctatgcatt actggcgaag caatatggtg
     3001 tgaaatggaa tggtcgcaaa tacgatccta aagactggga aaaaggcgat gttgtcaatc
     3061 gctgcatcag tgctgccaca tcatgcctgt acggtatatc tgaagcggcg gtattagccg
     3121 cgggatatgc gcccgctatt ggatttatcc atagtggcaa accgctttca tttgtttatg
     3181 acatagccga tatcattaaa tttgatttgg ttgtgccaaa ggcatttgaa atagcggcaa
     3241 gacacccggc agaacctgat aaagaagtga gactggcctg ccgtgatatt ttccgtagta
     3301 gtaaattaac gggcaaatta attccgttaa ttgaggaagt ccttgccgcc ggtgaaattg
     3361 aaccaccaca acctgcgccg gatatgttac caccagctat cccggaacct gaattgctag
     3421 gtgatagtgg tcaccggggg cgcagcggat gagcatggtc gtggttgtta cagaaaatgt
     3481 ccctccgcgc ttacgtggac ggcttgcaat ctggctcctg gaagtacgtg ccggtgttta
     3541 tgttggtgat acatcaaaac gtattcggga gatgatctgg cagcaaatct cccaactggc
     3601 aggttgcgga aatgtggtca tggcctgggc gaccaatacc gagtcgggtt ttgaatttca
     3661 gacctggggt gaaaacagac gtattccggt ggatttggat gggttacggt tggtttcgtt
     3721 tcttcctgtt gagaatcaat aagttacccg ttctttaaaa ataaggaaaa gtttgaattt
     3781 agttggtaga ttgttgttgt ggaataaaat tgtttaaaaa cagatacgta tgcttagtgt
     3841 gttccctgcg ccagcgggga taaaccggtg accataaggg catttcatac tgccttgctg
     3901 tgttccctgc gccagcgggg ataaaccggt gaacgtgaca acgcccgcga cagaaacgtc
     3961 gtgttccctg cgccagcggg gataaaccgg gaagaagcgg taaagcgggt tccagttcag
     4021 cgtgttccct gcgccagcgg ggataaaccg aaattaattt cgaaacgggc gttgatggtg
     4081 aagtgttccc tgcgccagcg gggataaacc ggctggcgca ccttacgcaa aaatagacgt
     4141 aaagtgttcc ctgcgccagc ggggataaac cggctgccgc gctttcggtg tgggagcagt
     4201 atgagtgttc cctgcgccag cggggataaa ccgaaactcc gtggtgtagt gcgtttgttg
     4261 gtgcggtgtt ccctgcgcca gcggggataa accgttggta ttaacgggaa tgtagtcact
     4321 ccaggagtgt tccctgcgcc agcggggata aaccggcgat cgtattttcc gctacgtcgc
     4381 tcaaatggtg ttccctgcgc cagcggggat aaaccgtcgt tgatggtgtg atgagtgtac
     4441 aaaaaaaagt gttccctgcg ccagcgggga taaaccgacg actgaatccg gcgaggctat
     4501 caactggccg tgttccctgc gccagcgggg ataaaccgcc gcacacgtcg agctggtggg
     4561 gattaatgct gtgttccctg cgccagcggg gataaaccgc agtaaacgtt cgcgcgttct
     4621 cgcgctcacg agtgttccct gcgccagcgg ggataaaccc tctgtgtatt cgctaccagc
     4681 cagcaccggt acgtgttccc tgcgccagcg gggataaacc gcagggtaac tggctgctcc
     4741 tcaaccattc cgcgtgttcc ccgcgccagc ggggataaac cggggtttta gcgaccggac
     4801 cagagtacgc aacagtgttc cccgcgccag cggggataaa ccgttgcgcc ttaagttcct
     4861 taacttcctc tgtatgtgtt ccccgcgcca gcggggataa accgggagtg atgaattgga
     4921 agtaacggat aaccgcgtgt tccctgcgcc agcggggata aaccggacgc actggatgcg
     4981 atgatggata tcacttagag ttccccccgc ccctgcggta gaactcccag ctcccatttt
     5041 caaacccatc aagacgcctt cgccagctct ttcaccagag gtagcattat ccgcataacg
     5101 tcacggcagc gacgttctat tcttccagga agtgccttat caatatgttg ttgattatcc
     5161 agtcttacgt catgccagct atttcccgca gggaatgcag ctgtttttgc gcgttgctga
     5221 taaccatcct tatttccaag attccagttg gtcgcctcca ccgaaagtac cgcaataccg
     5281 gctttgtcga aaacttcggc gtcattacaa cagccagtac ccttcggata atttttattc
     5341 aaacccggat tggtcgttgc ggcaattcca tgactacgcg caattgccag cgccctgtcg
     5401 cgcgttaatt tccttactgc ttcaggggtt tttacaccgc tgttgaaata caatttatcg
     5461 ccaacaatta agttatcgag attaatcacc agcagtgtat ttttcttttc ggagtcactc
     5521 atccgcttga gtaaattctc agcgcctaat ttcccttcct cttcgccgct ggtcgccaca
     5581 aatcgaatac catactcggt aggcgtattt ttcaggcgtt ctgccaattc cagcatgaca
     5641 cctaaacctg cggcgttatc atccattcct tgtaacgtca gcccgccgag attggcatcg
     5701 gcgtcagcat cgctcagcgg ggcgtaagta tccagatgcg ccataatgat gatctgctgc
     5761 ggcgctttgc cttcatgagc ggcaatcacc gtacttcccg tcacgttatg ccagctcttg
     5821 cgattatcgc gggcggtata aatataccga ctattaaatg tccgaatatc actgcgataa
     5881 cccatttgct gaaactgttg gcgaatataa tcggcagata acatttctgc tggagttccg
     5941 gtcatgcgtc ccggaaagaa agtagcaata tgtcgtgcct gggtattagc aaaatcgcca
     6001 ggtttaggtg acgaggcgtg tacggggaga ataaagcata cgccgagcgc cagggcagcg
     6061 gtacggtggc gcaatgcgga aaacatagtg agtccttaaa taccatgcaa atttttttac
     6121 cgccatagta tgaaactgcc gctgcgctaa aacaatttca aatcttccta aacgcccgaa
     6181 atccggtgcc ttaagcactt tttgatatta gctttgccaa atcgttattc cgttaaggaa
     6241 ctactcattc taattggtaa tttcattcgt tctcttacgc tccctatagt cgaaacatct
     6301 gatggcaaga aaatagcggt attgcaaagg aacggttatg gatcaaatac gacttactca
     6361 cctgcggcaa ctggaggcgg aaagcatcca cattattcgc gaggtggcgg cagaattctc
     6421 aaatccggtg atgctctact ctatcggtaa agattccagc gtcatgctgc atctggcgcg
     6481 caaggcgttt tatccaggta cgctgccttt cccgttgctg catgtcgata ccggctggaa
     6541 attccgcgag atgtatgagt tccgcgatcg tacggcgaaa gcctacggct gcgagctgct
     6601 ggtgcataaa aacccggaag gcgtggcgat ggggattaat ccattcgtac acggcagcgc
     6661 gaaacatacc gatattatga aaactgaagg cctgaaacag gcgctgaaca aatacggttt
     6721 tgatgccgcc ttcggtggtg cgcgccgtga cgaagagaaa tcccgcgcta aagagcgaat
     6781 ctactctttc cgtgaccgct tccatcgctg ggatccgaaa aatcagcgcc cggagctgtg
     6841 gcacaactac aacgggcaaa ttaacaaagg cgaaagcatc cgcgtcttcc cgctctctaa
     6901 ctggaccgag caggatatct ggcaatacat ctggctggaa aatatcgaca ttgtgccgct
     6961 gtatctcgct gcggaacgtc cggttctgga acgcgacggt atgttgatga tgattgatga
     7021 caaccgtatc gacctgcaac cgggcgaagt gattaaaaaa cggatggtgc gtttccgtac
     7081 gctgggctgc tggccgctga ccggtgcggt ggagtcaaat gcacaaacac tgccggagat
     7141 catcgaagag atgctggttt ccaccaccag tgaacgtcag ggccgcgtga ttgaccgcga
     7201 ccaggcgggg tctatggagc tgaaaaaacg tcaggggtat ttttaagatg aacaccgcac
     7261 ttgcacaaca aatcgccaat gaaggcggcg tcgaagcctg gatgattgcg caacaacata
     7321 aaagcctgct gcgttttctg acctgtggta gcgtcgatga cggcaaaagt accctgattg
     7381 gtcgcctgct acacgatacc cgccaaatct acgaagatca gctctcatcg ctgcataacg
     7441 acagtaagcg tcacggcacc cagggcgaaa agctggatct ggcactgctg gtggacggcc
     7501 tgcaagctga gcgcgaacag ggcatcacta ttgatgtggc ctaccgctat ttctctaccg
     7561 agaagcgtaa atttattatc gccgacaccc cagggcacga gcagtacacc cgcaatatgg
     7621 cgactggcgc atcgacatgt gaactggcga tcttactgat cgatgcccgt aaaggcgtgc
     7681 tcgatcaaac ccgtcgtcac agttttatct ccacactgtt ggggatcaaa catctggtcg
     7741 tggcgatcaa caaaatggat ctggtggatt acagtgaaga gacgttcacc cgtattcgtg
     7801 aagattatct gacttttgcc gggcagctgc cgggtaatct ggatatccgc tttgtgccgc
     7861 tctccgcact ggaaggcgac aacgtggctt cgcaaagtga aagtatgccg tggtacagcg
     7921 gtccgacact gctcgaagtg ctggaaaccg tggagatcca gcgagtggtg gatgcacagc
     7981 ctatgcgctt cccggtgcag tacgttaacc gcccgaatct cgattttcgt ggctacgccg
     8041 gaacgctggc atccggtcgc gtggaagtcg ggcaacgtgt aaaagtgttg ccctctggtg
     8101 tggaatcaaa cgtcgcgcgg atcgtgactt ttgatggcga tcgcgaagaa gcctttgccg
     8161 gagaagcgat caccctggtg ctgacggatg agatcgacat cagccgtggc gatctgctgc
     8221 tggcggcaga cgaagcgtta ccggctgtgc agagcgcgtc ggtggatgtg gtatggatgg
     8281 cggaacagcc gctttcccca ggccagagtt acgacatcaa aattgccggt aagaagacgc
     8341 gtgctcgtgt tgatggcatt cgttatcagg ttgatatcaa taaccttacc caacgcgaag
     8401 ttgaaaacct gccgctgaac ggcatcggcc tggtagatct cacctttgac gagccgctgg
     8461 tgttagatcg ttatcagcaa aacccggtta ccggtgggct gatttttatc gatcgcctga
     8521 gtaatgtgac cgtaggtgcc ggtatggtgc acgaaccagt tagccaggca actgctgcgc
     8581 catctgaatt cagtgcattc gaactggaat tgaatgccct ggttcgccgc catttcccgc
     8641 attggggtgc gcgcgattta ctgggagata aataatggct ctgcatgacg aaaacgtcgt
     8701 ctggcatagc cacccagtca ctgtgcaaca acgcgagcta caccacggtc atcgtggtgt
     8761 agtgctgtgg tttaccggcc tctccgggtc cggtaaatca acggtcgccg gggcgctgga
     8821 ggaggcgtta cataaacttg gcgtcagtac gtatctgctg gatggcgaca acgttcgcca
     8881 cggattatgc agcgatctcg gttttagcga tgccgaccgt aaagagaata tccgtcgcgt
     8941 cggtgaagtg gcgaatttga tggttgaagc cggactggtg gtgctgaccg catttatctc
     9001 gccacaccgc gccgaacgcc agatggttcg cgaacgcgta ggagaagggc gctttatcga
     9061 agtgtttgtc gatacgccgc tggcgatttg cgaagcccgc gatccaaaag gcttatataa
     9121 gaaagcgcgt gccggtgaac tgcgtaactt tacgggaata gattctgttt acgaagcgcc
     9181 tgaatcggca gaaattcatc tcaatggtga acaattagta acaaatttgg tacagcaatt
     9241 attagatctg ttgagacaga acgatattat cagatcctga gacaccaccg ggtttccatg
     9301 cccggttcgt caaagtaaca ggatcaaata tgcgtaatag ccataacatt acactaacaa
     9361 ataacgacag ccttactgag gatgaagaaa ccacatggtc actgcctggt gccgtggtcg
     9421 gttttatctc atggttattt gcgctggcga tgccgatgtt gatttatggc tctaacacgc
     9481 tgttcttctt tatctacacc tggcctttct ttctggcact gatgcctgtc gcggtagtgg
     9541 tggggattgc gctgcattca ttgatggacg gaaagctacg ctacagtatt gttttcaccc
     9601 tggtgacagt aggcattatg tttggcgcac tgtttatgtg gctactgggc taatttgtac
     9661 tttcttgtct cctctgttca taattcaaac cgtaactaat aatgagatta tgttctgcac
     9721 gccctgggta tacgtaacaa tggacaaatg tggtacattt gcccgcgttg tcgcggtatc
     9781 cccaacagag gatgtagagt cgtcttcgga tgcatgggat gatgatgccg tttttcaggg
     9841 ggcaggatgg gtaaactaac gctgctgttg ctggctattc tggtctggct acagtattcg
     9901 ctgtggttcg gtaagaacgg tatacatgac tatacccgcg tcaatgatga tgtggcggca
     9961 cagcaagcta caaacgctaa acttaaagcg cgaaacgatc aactttttgc cgaaattgac
    10021 gatctcaatg gcggccagga ggcgctcgaa gagcgtgcgc gtaatgaact cagcatgacc
    10081 aggccgggcg aaacttttta tcgtctggtg cctgacgcgt cgaagcgcgc acagtctgcg
    10141 gggcaaaaca atcgataaat cagcccagga attaacatgg caaccactca tttggatgtt
    10201 tgcgccgtgg ttccggcggc cggatttggc cgtcgaatgc aaacggaatg tcctaagcaa
    10261 tatctctcaa tcggtaatca aaccattctt gaacactcgg tgcatgcgct gctggcgcat
    10321 ccccgggtga cacgtgtcgt cattgccata agtcctggcg atagccgttt tgcaaaactt
    10381 cctctggcga atcatccgca aatcaccgtt gtagatggcg gtgatgagcg tgccgattcc
    10441 gtgctggcag gtttgaaagc cgctggcgac gcgcagtggg tattggtgca tgatgccgct
    10501 cgtccttgtc tgcatcagga tgacctcgcg cgattgttgg cgttgagcga aaccagccgc
    10561 acgggaggga tcctcgccgc accagtgcgc gatacgatga aacgtgccga accgggcaaa
    10621 aatgccattg ctcataccgt tgatcgcaac ggcttatggc acgcgctgac gccgcaattt
    10681 ttccctcgtg agctgttaca tgactgtctg acgcgtgctc taaatgaagg cgcgactatt
    10741 actgacgaag cctcggcgct ggaatattgc ggatttcatc cgcagttggt cgaaggccgc
    10801 gcggataaca ttaaagtcac gcgcccggag gatttggcgc tggccgaatt ttacctcacc
    10861 cgaaccatcc atcaggagaa tacataatgc gaattggaca cggttttgac gtacacgcct
    10921 ttggcggtga aggcccaatt atcattggtg gcgtgcgcat tccttacgaa aaagggctgc
    10981 tggcgcattc tgatggcgac gtggcgctcc atgcgttgac cgatgcattg cttggcgcgg
    11041 cggcgctggg ggatatcggc aaactgttcc cggataccga tccggcattt aaaggtgccg
    11101 acagccgcga gctgttgcgc gaagcctggc gtcgtattca ggcgaaaggt tatgccctcg
    11161 gcaacgtcga tgtcactatc atcgctcagg caccgaagat gttgccgcac attccacaaa
    11221 tgcgcgtgtt tattgccgaa gatctcggct gccatatgga tgatgttaac gtgaaagcca
    11281 ctactacgga aaaactggga tttaccggac gtggggaagg gattgcctgt gaagcggtgg
    11341 cgctactcat taaggcaacg aaatgattga gttcgataat ctcacttacc tccacggcaa
    11401 accgcaaggc accgggctgc tgaaagcgaa tccggaagac tttgtggtgg tggaagattt
    11461 gggctttgag cctgatggtg aaggtgagca tattctggtt agaatcctca aaaacggctg
    11521 caatacccgt tttgtggcgg atgcactggc gaaattcctg aaaattcatg cccgtgaagt
    11581 cagcttcgct gggcaaaaag ataaacatgc tgttacggaa cagtggttat gcgctcgcgt
    11641 gccgggcaaa gagatgcccg atctgagcgc ctttcaactg gaaggctgcc aggtgctgga
    11701 gtatgcgcgg cacaagcgca agctgcgttt aggggcgctg aaaggtaacg cctttaccct
    11761 ggtactacgc gaagtgagca atcgcgatga cgttgaacaa cgtctgatcg atatttgcgt
    11821 aaaaggtgta ccgaactact tcggtgccca acgttttggg attggcggta gcaacttgca
    11881 gggcgcgctg cgctgggcgc aaaccaatac tccggtgcgc gatcgcaata aacggagttt
    11941 ttggttgtcg gcagcccgca gtgcgttgtt taatcagatt gttgctgagc gcctcaaaaa
    12001 agcagacgtt aatcaagttg ttgacggcga tgcgctacaa ttagccggac gtggtagctg
    12061 gtttgttgca accaccgaag aagtggcgga attacagcgt cgcgtcaacg ataaagagct
    12121 aatgataacc gccgcattgc cgggcagtgg cgaatgggga actcagcgtg aagcgctggc
    12181 attcgaacaa gcagctgtcg ccgcagaaac tgaattacaa gctttactgg tgcgcgaaaa
    12241 agttgaagcc gcgcgcagag cgatgctgct gtatccgcaa caattaagct ggaactggtg
    12301 ggatgacgtc accgtagaaa tccgtttctg gcttccggcg ggtagttttg caaccagcgt
    12361 tgtcagggaa cttatcaaca caacaggtga ttatgcgcat attgctgagt aatgatgacg
    12421 gggtacatgc acccggtata caaacgctgg cgaaagcctt gcgtgagttt gctgacgttc
    12481 aggtggtcgc ccccgatcgt aaccgcagcg gcgcttcaaa ttctctgaca ctggaatcct
    12541 ccctgcgcac gtttaccttt gaaaatggtg atattgctgt gcaaatggga accccgaccg
    12601 attgcgtcta tcttggcgtg aatgctctga tgcgtccgcg cccggacatt gttgtgtccg
    12661 gaattaacgc cgggccgaat ctgggggatg atgttattta ttccggtacg gtagccgccg
    12721 cgatggaagg ccgtcattta ggttttccgg cgctggccgt ctcgcttgac gggcataaac
    12781 attacgacac tgccgcggcg gtaacctgtt caattttgcg cgcactgtgt aaagagccgc
    12841 tgcgcaccgg acgtattctt aacattaacg ttccggattt accattggat caaatcaaag
    12901 gtattcgcgt gacgcgctgc ggtacacgac atccggcaga tcaggtgatc ccgcagcaag
    12961 atccgcgcgg caatacgctg tactggattg gcccgccggg cggtaaatgt gatgccggtc
    13021 cggggaccga ttttgctgcg gtagatgagg gctatgtctc catcacgccg ctgcatgtgg
    13081 atttaactgc gcatagcgcg caagatgtgg tttcagactg gttaaacagc gtgggagttg
    13141 gcacgcaatg gtaagcagac gcgtacaagc acttctggat caattacgtg cgcaaggtat
    13201 tcaggatgag caggtgctga atgcacttgc cgccgtgccg cgtgaaaaat tcgttgatga
    13261 agcgtttgaa caaaaagcct gggacaatat cgccttgccg ataggtcagg ggcagacaat
    13321 ttcgcagccg tatatggtgg cgcgaatgac cgaattactc gagctgacgc cgcagtcgcg
    13381 ggtgctggaa attggcaccg gttcgggata tcaaacggca atcctggcgc atcttgtcca
    13441 gcatgtttgc tcggttgaac ggattaaagg cttgcagtgg caggcacgtc gccgcctgaa
    13501 aaatcttgat ttacataatg tttcaacccg tcatggcgat ggatggcaag gttggcaggc
    13561 acgtgcgccg tttgacgcta tcattgttac ggcggcaccg ccggaaattc caactgcgct
    13621 aatgacgcag ctggacgaag gcgggattct cgtcttaccc gtaggggagg agcaccagta
    13681 tttgaaacgg gtgcgtcgtc ggggaggcga atttattatc gataccgtgg aggccgtgcg
    13741 ctttgtccct ttagtgaagg gtgagctggc ttaaaacgtg aggaaatacc tggatttttc
    13801 ctggttattt tgccgcaggt cagcgtatcg tgaacatctt ttccagtgtt cagtatggtg
    13861 ccttgcacgg taattatgtc actggttatt aaccaatttt tcctggggga taaatgagcg
    13921 cgggaagccc aaaattcacc gttcgccgca ttgcggcttt gtcactggtt tcgctatggc
    13981 tggcaggctg ttctgacact tcaaatccac cggccccggt cagctccgtt aatggcaatg
    14041 cgcctgcaaa taccaattct ggtatgttga ttacgccgcc gccgaaaatg gggacgacgt
    14101 ctacagcgca gcaaccgcaa attcagccgg tacagcagcc acaaattcag gccactcaac
    14161 aaccgcaaat ccagccggtg cagccagtag ctcagcagcc ggtacagatg gaaaacggac
    14221 gcatcgtcta taaccgtcag tatgggaaca ttccgaaagg cagttatagc ggcagtacct
    14281 atacagtgaa aaaaggcgac acacttttct atatcgcctg gattactggc aacgatttcc
    14341 gtgaccttgc tcagcgcaac aatattcagg caccatatgc gctgaacgtc ggtcagacct
    14401 tacaggtggg gaatgcttcc ggtacgccaa tcactggcgg aaatgccatt acccaggccg
    14461 acgcagcaga gcaaggagtt gtgatcaagc ctgcacaaaa ttccaccgtt gctgttgctt
    14521 cgcaaccgac aattacgtat tctgagtctt cgggtgaaca gagtgctaac aaaatgttgc
    14581 cgaacaacaa gccaactgcg accacggtca cagcgcctgt aacggtacca acagcaagca
    14641 caaccgagcc gactgtcagc agtacatcaa ccagtacgcc tatctccacc tggcgctggc
    14701 cgactgaggg caaagtgatc gaaacctttg gcgcttctga ggggggcaac aaggggattg
    14761 atatcgcagg cagcaaagga caggcaatta tcgcgactgc agatggccgc gttgtttatg
    14821 ccggtaacgc gctgcgcggc tacggtaatc tgattatcat caaacataat gatgattacc
    14881 tgagtgccta cgcccataac gacacaatgc tggtccggga acaacaagaa gtgaaggcgg
    14941 ggcaaaaaat agcaaccatg ggtagcaccg gaaccagttc aacacgcttg cattttgaaa
    15001 ttcgttacaa ggggaaatcc gtaaacccgc tgcgttattt gccgcagcga taaatcggcg
    15061 gaaccaggct tttgcttgaa tgttccgtca agggatcacg ggtaggagcc accttatgag
    15121 tcagaatacg ctgaaagttc atgatttaaa tgaagatgcg gaatttgatg agaacggagt
    15181 tgaggttttt gacgaaaagg ccttagtaga agaggaaccc agtgataacg atttggccga
    15241 agaggaactg ttatcgcagg gagccacaca gcgtgtgctg gacgcgactc agctttacct
    15301 tggtgagatt ggttattcac cactgttaac ggccgaagaa gaagtttatt ttgcgcgtcg
    15361 cgcactgcgt ggagatgttg cctctcgccg ccggatgatc gagagtaact tgcgtctggt
    15421 ggtaaaaatt gcccgccgtt atggcaatcg tggtctggcg ttgctggacc tgatcgaaga
    15481 gggcaacctg gggctgatcc gcgcggtaga gaagtttgac ccggaacgtg gtttccgctt
    15541 ctcaacatac gcaacctggt ggattcgcca gacgatcgaa cgggcgatta tgaaccaaac
    15601 ccgtactatt cgtttgccga ttcacatcgt aaaggagctg aacgtttacc tgcgaaccgc
    15661 acgcgagttg tcccataaac tggaccatga accaagtgcg gaagagatcg cagagcaact
    15721 ggataagcca gttgatgacg tcagccgtat gcttcgtctt aacgagcgca ttacctcggt
    15781 agacaccccg ctgggtggtg attccgaaaa agcgttgctg gacatcctgg ccgatgaaaa
    15841 agagaacggt ccggaagata ccacgcaaga tgacgatatg aagcagagca tcgtcaaatg
    15901 gctgttcgag ctgaacgcca aacagcgtga agtgctggca cgtcgattcg gtttgctggg
    15961 gtacgaagcg gcaacactgg aagatgtagg tcgtgaaatt ggcctcaccc gtgaacgtgt
    16021 tcgccagatt caggttgaag gcctgcgccg tttgcgcgaa atcctgcaaa cgcaggggct
    16081 gaatatcgaa gcgctgttcc gcgaataagt aagcatctgt cagaaaggcc agtctcaagc
    16141 gaggctggcc tttttctttt atttgggagt cggtgtgttt tgtcgcctgg ataagacacg
    16201 tcagcgtccc acccggcaat caacatccta tttgctcatc atcttgcgca ccagctgcat
    16261 aaacagctcc tgctcttctg cactcaaacg ccccaaaaat tcctcatcca cactgtcacc
    16321 aatcggtatc gccgcagcaa gtaccttttc tccttcggca gtcagccaga caaagcgtcg
    16381 ccgcttatca gcagagtcat gttctcgtct gactaagcca cgattttcca ttcgtgccaa
    16441 catttctgcc agcgttgctt tggtgctgac ggctgcttct atcagtgcca cctgttcgat
    16501 accaggttta tcagcaatgg cgcgcatcac cgcgtactgt ggtttggtca gatcaggtaa
    16561 ctcgtgctgc cagcgagccg tgtgctgctg aaaaagctgt cgtaactgat ggaacgcttt
    16621 atttcgtaac gccatgtaaa ctccgggcaa actatctgcc gctatcatag cggtttttca
    16681 gtgacgaaga agatgttgtt acttttcagc gcttgccgcg atgaaaacag aggcgtattt
    16741 taataataac gttcgtatac gaacaattta gagagtaagc aatgaaactg atcgtcggga
    16801 tgacaggggc taccggtgcg cctcttgggg tggcattact gcaagcgctg cgggagatgc
    16861 cgaatgtcga gactcatctg gtgatgtcga agtgggcgag aaccaccatt gaactggaaa
    16921 cgccttacag cgctcgcgat gttgctgccc tcgcagactt cagccataac ccggcggatc
    16981 aggcggcgac catctcctca ggttcctttc gtaccgacgg catgatcgtt attccgtgca
    17041 gtatgaaaac gctcgccggt atccgtgccg gttacgccga tggcctggtg gggcgcgcgg
    17101 cggacgtcgt gctcaaagaa ggccgcaaac tggtgctggt gccgcgtgaa atgccgctta
    17161 gcacaatcca tctcgaaaat atgctcgcac tttcgcgcat gggcgtggcg atggtgccgc
    17221 cgatgcctgc cttttataac catcccgaaa cggtagatga cattgtccac cacgtggtag
    17281 cccgcgtgct ggatcaattt ggcctcgaac atcctcacgc caggcgctgg caaggattgc
    17341 cgcaggcccg gaatttttct caggagaatg aataatggca tttgatgatt tacgcagctt
    17401 tttacaggcg ctcgacgacc atgggcaatt gctgaaaatc agcgaagagg tgaacgcaga
    17461 accggatctg gcagccgcag ccaacgccac cgggcgtatc ggcgacggcg cgcccgcgct
    17521 gtggtttgac aatattcgcg gctttaccga tgcccgcgtg gcgatgaaca ccatcggttc
    17581 ctggcagaac cacgcgattt ccctcggcct gccgccaaat accccggtta aaaaacagat
    17641 cgatgagttt atccgccgct gggacaactt cccgatagcc ccggaacgcc gagccaatcc
    17701 agcctgggcg cagaacaccg tcgatggcga aaagattaac ctgttcgata tcctgccgct
    17761 gtttcgttta aacgacggtg atggcggttt ctatctcgac aaagcgtgcg tggtttcccg
    17821 cgatccgctc gacccggata acttcggcaa gcagaacgtc ggtatctacc gcatggaagt
    17881 gaagggcaaa cgtaagctcg gcctgcaacc ggtgccgatg cacgatatcg cgctgcatct
    17941 gcataaagcg gaagagcgcg gtgaagatct gccgattgcc atcaccctcg gtaacgatcc
    18001 gatcatcacc ctgatggggg ccacgccgct gaaatatgat cagtccgagt acgaaatggc
    18061 gggcgcgctg cgcgaaagtc cgtacccgat tgccaccgcc ccactgaccg gttttgatgt
    18121 accctggggc tcggaagtga tcctcgaagg ggttatcgaa agtcgtaaac gtgaaatcga
    18181 agggccgttc ggtgagttta ccgggcacta ctccggcggg cgcaacatga ccgtggtgcg
    18241 catcgataac gtctcttatc gcagcaaacc catttttgaa tcgctctatc tcgggatgcc
    18301 gtggacggaa atcgactacc tgatggggcc agccacctgc gtgccgctgt atcagcaact
    18361 gaaagccgag ttcccggaag tgcaggcggt aaacgccatg tacacccacg gcctgctggc
    18421 gattatctcc accaaaaaac gctacggtgg ctttgcccgc gcggtgggcc tccgtgcgat
    18481 gaccacgcca cacggtctgg gctatgtgaa gatggtgatt atggtcgatg aagacgttga
    18541 tccgttcaac ctgccgcagg tgatgtgggc gctctcttcg aaagtgaacc cggcagggga
    18601 tttggtgcag ttgccgaata tgtccgtgct ggaactcgac cctggctcaa gtccggcggg
    18661 gatcaccgac aagctgatta tcgacgccac cacgcctgtc gccccggaca accgtggtca
    18721 ctacagtcag ccggtggtgg atttaccgga aaccaaagcc tgggctgaaa aactgaccgc
    18781 tatgctggcc gcacgtcaat aaggagaaca agatgatttg tccacgttgt gctgatgaac
    18841 agattgaagt gatggcgaaa tcgccggtaa aagatgtctg gacggtctac cagtgccagc
    18901 attgcctcta tacctggcgc gataccgaac cgctgcgccg caccagccgc gaacattatc
    18961 ccgaagcgtt ccgcatgacg cagaaagata ttgatgacgc gccgatggtg ccgagcatcc
    19021 cgccgctgct ggcggaaggt aagtgttaat aaaaaagtgc cgggtagagg ctttgcttta
    19081 cccggccacg ttatttaatt ttataaaacg ataaccgtct gctttccggc gagccggtat
    19141 caatataaac ctgattggca aatgtctgga tgttatcaaa catcatatgt ccaaagataa
    19201 aacagtcggc accgtttatt tcttttaact ctcccttgaa tgatttctgc acgcgatcaa
    19261 caggccagag taattcgctt tctgctattt ctttaccaaa ctgatattca tcacccggat
    19321 aatccgcatg tgcgatgaca tattttattc tgtcgttagt gatttcaata atatgtggaa
    19381 gattatgaaa acgtagcaac aggtctattg cttctttttg ttccgtatca cttaactcaa
    19441 agaaccagtc gccaccgctg gcaagccaca tattgccatc gccggtcgcg aacgcatcca
    19501 acgccatcgc ttcatggttg cctttaaccg atgtaaacca gggctggttt aacaggcgca
    19561 gcacgttaag actctccggc ccacgatcga tgttatcgcc taccgaaata agtaagtcgg
    19621 tttcagggca aaaagagagt tgatgtaagc gggattgtaa taattgatag tcaccatgaa
    19681 tatcaccaac ggcccatata tggcgatagt gatggacatt gattttttga tagcgtgtag
    19741 atggcatggt tttaccctgt aaaataagat ttcctattat acagggtatt tttatttgat
    19801 tcgtcagttg tcgttaatat tcccgatagc aaaagactat cgggaattgt tattacacca
    19861 gactcttcaa gcgataaatc cactccagcg cctgacgcgg agtgagtgaa tctgggtcca
    19921 ggttttccag tgcctcgacc gcaggcgaag tttcttccgg tacggatagc aaagacattt
    19981 gtgtaccatc cacttgcgta gcagcggcgt tcggcgaaat gctttccagc tcacgcagtt
    20041 tttgccgtgc gcgcttaata acctcttttg gcacgcctgc cagcgctgca accgccaagc
    20101 cgtagctttt gcttgccgcg ccatcctgca cgctatgcat aaaggcaatg gtgtcgccgt
    20161 gctccagcgc atcgagatgc acgttggcga cgccttccat tttctccggc agttgggtca
    20221 gctcgaaata gtgggtagca aacagcgtca acgctttgat cttatttgcc agattttccg
    20281 cgcatgccca cgccagcgac agaccatcgt aagtggacgt tccacgccca atctcgtcca
    20341 tcaacaccag actgtattcg gtggcgttat gtaaaatatt ggcggtttcg gtcatctcca
    20401 ccataaaggt tgaacgtccg gaagccagat catccgcggc acctacgcgg gtaaagatgc
    20461 gatcgatcgg gccaatctcg actttttgcg ccggtacgta gctgccgata taggccatca
    20521 gcgcaatcaa cgcggtctgg cgcatatagg tacttttacc gcccatgttc ggaccggtga
    20581 tgatcaacat gcggcgctgc ggcgacaggt tcagcgggtt ggcgataaac ggctcattca
    20641 gtacctgttc aaccaccgga tggcggcctt cggtaatgcg aatgccaggt ttatcaataa
    20701 aggtcgggca ggtgtagttc agggtatagg cccgttccgc caggttcacc agcacgtcga
    20761 gttccgccag cgcgctcgcg ctctgttgca acgcttccag atgcggcaac agcaagtcga
    20821 acagctcttc ataaagttgt ttttccagag ccagtgcttt gccttttgag gtgagaactt
    20881 tatcttcgta ctctttcagc tccggaataa tgtaacgctc ggcgtttttc agcgtctggc
    20941 ggcgcatgta gttaatcggt gccagatggc tttgcccacg gctgatctga atgtagtagc
    21001 cgtgcactgc attaaagcca actttcagcg tgtccaggcc ggtacgttca cgctcgcgga
    21061 cctccagacg ctccagataa tcggtcgcgc cgtcagccag cgcgcgccac tcatccagct
    21121 cttcgttata gcccggtgcg ataacaccac cgtcgcgtac cagcactggc ggtgtgtcga
    21181 tgattgctcg ctccagcaga tcgcgcagct cggcaaactc gcccattttt tcacgcagcg
    21241 cctgtaccgg agcgctatcg acattttcta atagtgcacg cagctccggc agttgctgga
    21301 aagcgtgacg catacgggcc agatcgcgtg ggcgagcagt tcgtaaagcc agacgtgcca
    21361 gaatacgttc caggtcgccg acctgacgca gtaccggctg cagctcggcg gtgaaatcct
    21421 gcaatgcgcc aatagtttgc tggcgctcaa gcaacacgcg ggtatcgcgc actggcatat
    21481 gcagccagcg tttcagcata cggctgccca tcggcgtgac ggtgcagtcg agcacagaag
    21541 ccagcgtatt ttccgcacca cccgccagat tctgggtgat ttccagatta cgacgcgtcg
    21601 cggcatccat aatgatgctg tcctgctcac gttccatggt gatagaacga atatgcggca
    21661 gggtcgtgcg ttgggtatct ttcgcatact gcaacagaca accggcagca caaagtccgc
    21721 gcggcgcgtt ctcgacgcca aaaccgacca gatcgcgagt gccaaattgc agattcaact
    21781 gctggcgcgc ggtgtcgatt tcaaactccc acagcgggcg acggcgcagt ccgcgacgac
    21841 cttcaatcag cgacatctcg gcgaaatctt ctgcatacaa cagctctgcc ggattagtgc
    21901 gttgcagctc tgccgccatc gtttcgcggt cagccggttc gctcaggcga aaacgcccgg
    21961 aactgatatc cagcgtcgcg tagccgaaac ctttgctgtc ctgccagata gccgccagca
    22021 ggttatcctg acgctcctgc aacagcgctt catcgctgat ggtgcccggc gtaacgatac
    22081 gcacaacttt gcgctcaacc ggacctttgc tggtcgccgg atcgccaatt tgttcgcaga
    22141 tggcaacgga ctcgccctga ttcaccagtt tggcgaggta gttttccacc gcatggtagg
    22201 ggatccccgc catcgggatc ggctctcccg ctgaagcacc gcgtttggtc agtgaaatat
    22261 ccagcagttg cgaggcgcgt tttgcatcgt cataaaatag ttcataaaaa tcacccatcc
    22321 ggtaaaacag caggatctcg ggatgctggg ctttcagctt gagatattgc tgcatcatgg
    22381 gcgtatgggc atcgaaattt tctattgcac tcatggggtt atgtccggtt ccctgatatt
    22441 aaatggggtg tgatggtttt tattttcgtt gtaatcacat aaggcgcaca tgataccgga
    22501 gttaatcatt tcaggggaag aagcgaacgg cgagttcttg atggtgattc cgggaaagat
    22561 gctgttttgc agcttttgcg tgatgcttat gaagcaagta cgcaaaattg cgtcggttga
    22621 ataaacctcc tccttcagag ccatagtccc ctttcaaatt aagttgtaat ataaatacac
    22681 cttaagagta tggaggcagc atgtccggca agcgtatctc tcgtgaaaaa ctgacgatta
    22741 aaaaaatgat cgatctttat caagcgaaat gcccgcaggc gtcagcggag ccggagcatt
    22801 acgacgcgct gtttgcctac gcgcaaaagc ggctggataa atgtgtgttc ggcgaagaga
    22861 aaccagcctg taagcagtgt ccggtgcact gttaccagcc agcaaagcgt gaagagatga
    22921 aacagattat gcgttgggcc gggccgcgaa tgctgtggcg tcatccgatc ttaaccgtgc
    22981 gtcatctcat cgatgacaag cgtccggtgc cggagttgcc agaaaaatat cgtccaaaga
    23041 aataaatctg ccggagagcc atactctccg gcaaataagg cgtttacgct ttggtggcaa
    23101 tcaggatggc tgatgctttg atcaatgcaa taacgcgcga gccagtggtg agtttcattt
    23161 catcctgact ttcatttgtt accactgcca caatttcaaa acccgcatcc gttttgatat
    23221 ggacagtggc gttcactgcg ccttcggtga ttgtcgacac gctgccagca aactggttgc
    23281 gagcagagaa tttcagtcca cagtcttccg ttgccagtgt tacccacggg gctttaatca
    23341 gggcaatcgc ttctttccct tttgccagac caagagcctg ctggctgctg tgtgtaacaa
    23401 ttgccaccag ctttgcacca cctgccagcg tcagttctac ttcatcattc acagcgccca
    23461 ttgctactgc gctaactgtt ccggttaatt gatttcttgc tgaaactgcc atactgcctc
    23521 ctttcattga atgttgatcg agtataacca catctgttaa atcaatgccg atttatcaat
    23581 tcccagtcgc ttcatccgtg acagcagagt cgtgcgtttc agccccagac gttgcgcagc
    23641 gcctttaggc ccggcaacca cgccgtttgt ttctttcagc acgcgaacaa tcaactgata
    23701 ttcatcttcg ccctcctggg cgacaaccgt tgcggcaggc ggcgtttcag gttctggtaa
    23761 agcaatatct ggcaatgaca gctgcagcac gttgccgcgt gttagcaata ccgcgcgctc
    23821 gatgacgttt tccagctcac gcacgttgcc cggccattcc atgttgctca ggatgcgcag
    23881 cgtctcggca ggaatgctgt cgatattgcg ccccagacga cgggcaattt tgaaggtaaa
    23941 ggctttcgcc agcagcggaa tatcttccgg acgctcgcgt agtggcggca ggtggatcgg
    24001 gaatacgttc aggcggtaat agagatcact acggaactct cggtcggcga ccattttttt
    24061 cagatcgcgg ttagtcgcgg caattaaacg tacgtccgtc tgaatgattt tgttgctgcc
    24121 gaggcgttca aactcctgtt cctgtaatac acgcagcaac ttcggctgta actccagcgg
    24181 catatcgccc acttcgtcga ggaacaggga gcttttatcc gccagttcaa aacgaccaat
    24241 gcgctgggcg ctggcaccgg taaaagcccc acgctcatga ccaaacagat cgctctccag
    24301 caatccggca ggcatcgccg cgcagttcat ttttaccatg cggcgattat tacgcccact
    24361 gagattatgg atagcacggg caatcagctc tttacccgtg ccagtttcac cgaggatcag
    24421 cacggtactg tcactttgcg ccaccatttc aacttgttta agcacgctgt acatggcttc
    24481 gctgcggcca ataatctcgc caaattcact atcaacattg ttgagctgct cggtcagggc
    24541 gaggttttca tcaaccagcc gttctttcag acgatggatt tcctgatagg cgagggcgtt
    24601 atcgacagcg attgccacac gttcggcaat ctggcgcagt aaattcagat tggtagtggt
    24661 aaacactttc tcttcgcatt gcgccagttt cagcacgccc agcatggtgt cgccagacat
    24721 cagcggtaac aggcacaagg tttgaatctg gttgccccag gtgtcgaaca acatgcgttc
    24781 atagggggct aaatcgtccc gctcgtggag attaatcaac agcatctctt tacttttgaa
    24841 gacgcgttcg gtgagggttc cggcttcatc gacttcgctc tgttcgtggg cgggatgctg
    24901 tttatcaaga tagtgagtgg agtagatgtt gagtttgttt ttacggtggc tgcgtaagac
    24961 gatgctgata tcgtcaatgt cgaagtagta atggatttct ttggcgactt cgctgaccag
    25021 ttcgtccata tccaggcggg aaagcaccgc gttggtaatg gcaaccagga tgcggaagtt
    25081 atcgcgctcc cggcataaca actcatagtc gacattgtta acgacgcggc tctggatttg
    25141 ttcggtgacg accgaaacga tctgcgtaaa tgtttgcaga cgattgaact ctttttcgct
    25201 ccaggggcga tcgtcataac gaataaattc acagccacca aaaatatgcc cttccgccgc
    25261 cagtggcatc aggcaatagt gaccaaattt ggggtatagt ccacctgcgg ccagctgcgg
    25321 ccaggtttca caaaattctt cgtaactgca atgcagcgta tcagggcgcg acaaaatgct
    25381 gcgtaccgga ccgtgtgcca gaacagtttc gtcttcatat ttaatggggg tgtctttttc
    25441 acgcgacgcg taataagacg cacgttgagt ctgcgcttgc cacaacacaa tagccgcgtt
    25501 gtcggcgagc gcagaacgct ttaccagttg cgaaagagcc tcacacagcg aggccagatc
    25561 gggctgctgc aatagtgtcc gagtgatgtc gaacaaccct tgttgtccga gatcactcat
    25621 cggtgtatat gacattttgt tcttccagga aagcaccgca aagcggtgca aaaggtcatt
    25681 gactaactat aggagattta gaattttatt agcatatacg cggaagcggt tcggcgtgtg
    25741 gtaaatcgag ggttcgtttc acgccataca gaccggcaag acgaacacct ttacgttcca
    25801 ccacttcacc aatcagcgcc gcgtctttcc ccagtggatg ggaatgtaac gctgccagca
    25861 cttgctctgc cgcgttgcgt tcaacggcga tcaccagttt gccttcgttg gcaaagttaa
    25921 gggcgtccag tcccagcagc tcgcaaacgc cacgcacagc aggtttaacc ggcagtgccg
    25981 cttctgaaag ttcaatacca caaccgcagg ctgccgcgaa ctcatgtacc accgcgttta
    26041 caccaccacg ggtggcatca cgcagcgctt tcacgccggg aatgtcacgc agcgtctgaa
    26101 taagcggcgt tagtaccgca cagtcgctga ccagttcgcc atccagcccc agctgctcac
    26161 gcaggttaag gatagtcgcc ccgtggtcgc cgagagttcc actcaccagc agaacatcgc
    26221 ctgcggttag cgtttgtgcg ccccagtgaa tattcgccgg aattgcgccc atgccagcgg
    26281 tgttgataaa cagtttatct gccgcgccgc gctgcaccac tttagtgtcg ccagtaacga
    26341 tggcaatgcc tgccgcgcgg gcggtttctg ccatgctggt cactacggct ttcagtgtct
    26401 ccatcggcaa tccttcttcg aggataaagc cacaggagag atagcgcgga atagcgccac
    26461 tgaccgcaac gtcattcgcg gtgccgcaaa tcgccagctt gccgatatta ccgccaggga
    26521 agaacagcgg gtcaataacg taactgtcgg tggagaacgc cagacggtcg ccttccgcta
    26581 ccagctgcgc cagatcaaga cgtgcctgat cttcctgctc tgccagccac gggttggcaa
    26641 aggcttccat aaacaggctg ttgattaatt gctgcatcgc ctggccgccg ctaccgtggg
    26701 cgagttggat attattcacg cttcactctc ctgctgacga tactgatacc acgcggcgca
    26761 cgctccttcg gaggaaacca tcagcgcacc aaacgcggtt tgaggattac aggtgttacc
    26821 aaacagcggg cattgatgcg gcttacattt gcccgttaat acttcgccac aacgtgcgcg
    26881 cgggtcatcg cagacctgct gcggtgccgg gcggaaatgc gcttcggcat cgaatcgttg
    26941 ataatccggc gtcaggtgta caccagaaga ttcaatcacg cctaagccgc gccattcgct
    27001 gtcgccgttg acacagaaca catcggcaat cgcctgttgc gccagcaggt taccggcatc
    27061 cggcaccacc cgacgatact gattctctac cttgctgtgg gccgctattt tctgctccac
    27121 cagcatgacc acgccttgca gtagatcaag cggttcgaaa ccagccacca ccagcggacg
    27181 atgaaaatcg ctggcgataa aattataggc atcagtgccg atcaccatac tgacgtggcc
    27241 gggcgcgagg aacgcgtcga taccgttatc cggctgttcc agcaaactgc gcagtgttgg
    27301 gatgagcgta atgtgctggc agaagaagta aaaattctgc acatcgcggg cttttgcctg
    27361 ttgcagagtg atggcggtgg tcggcatggt ggtttcaaaa cccaagccga agaacaccac
    27421 tttgcgggtt ggattctcct gcgccagttt caacgcatcc atcggcgagt aaacgatgcg
    27481 cacatcggca ccgcgtgcct ttgcctgtaa cagcgaaccc tgtttccctg gtacgcgcat
    27541 ggcgtcgcca aaggtacaga agatgacttc cggatggctg gcaatctcca cgcaggtgtc
    27601 gattctaccc atcggcagta cacacaccgg gcaaccggga ccgtggataa actcaacgtt
    27661 ttccggcagc aactggtcga ggccgaattt aaaaatggcg tgggtatgac cgccacacac
    27721 ttccataatc cgcagagggc gttcggcggt gtaagagaga tgtgaagcac gttcgcgcag
    27781 atgctcaatt aactgcatca cctgttccgg cgcgcgatat tcatcaacaa aacgcattat
    27841 ttttcctcgc catacaacag cgcaccgaca tccggctcaa cgtcaaacat gttttgtaag
    27901 gcgtcgagag tgtcgcgcgc ttcggcttca ttaattacgc tcatggcaaa gccaacgtga
    27961 accagtaccc actggcctac gcgtggctga ccgttttcat cgcagctgcc gactaacgtt
    28021 aaatcgacat cgcgctgaat gccgcagacg tcgactttcg cctggttgcc gtcaatggtg
    28081 cggatctggc cgggaacgcc tatgcacatc gctgtgtctc cagccagttc agccactggt
    28141 ccatcccttc gccgctggtg gcggaaataa ggatgatttc aatttctgga ttgacttcgc
    28201 gggcgcaggc gatgcacttc tcaacgtcaa agttgagata cggcagcagg tcaactttgt
    28261 tgagcagcat cagtgaggcg gcggcaaaca tatgtggata tttcagcggt ttgtcttcac
    28321 cttcggtaac ggaaagcacc gccactttgt gtttttcacc gagatcgaag ctcgccggac
    28381 atacgaggtt gccaacgttt tcgataaaca gaataccgtt atcgtccagt ggcaggcgcg
    28441 gcgcggcgtc ggcgatcatc tgtgcgtcaa gatggcaacc tttaccggta ttcacctgaa
    28501 tcgctggtgt gccggtagca cgaatgcgtg cggcatcgtt cacggtttgc tgatcgcctt
    28561 caataaccgc gcacggaacg ctgtctttca ggcgcattaa ggtttccgtc agcagggtgg
    28621 ttttaccgga accagggctg gaaaccaggt tgagcaccag ttgcttgcgg gcagcaaagc
    28681 gcgcgcggtt gcgttcagcc agacggttgt ttttgtccag cacgtcaatt tcgacttcca
    28741 gcatccgacg ctggctcatg cccggcgcgt gagtgcccgc ttcgccatga ccgtaatgca
    28801 ggtcgccttc ttcagtctgg ctgggggtaa attcaggcgc tttaatgccg gtgattttca
    28861 tcttcgggcg tgccgccggg gcaaatggcg cgctacgaaa cgcggaatga gggttatgtt
    28921 catcaccctc aatatagagg ttgccttcac cgcaaccgca tgttgtacac atcgctcact
    28981 cctggtctat ttctatccgc cgaatctgta aaccgtcgtc tgccacaatc tgtagcatgt
    29041 caccatgaca ctgtggacag cggcggacgc gctgggtcag tagcgtcaca tactgttggc
    29101 atgtttcaca ccagcattcg gcctcttgtt cttcgaggtg cagtttacaa ccttccgcca
    29161 tgctgccgcg gcaaaccaga tcaaaacaaa aggcaagaga gctggtttcg acacaagaaa
    29221 atgcgccaat tttgagccag accccagtta cgcgttttgc gccgtgtttt gcggcctgct
    29281 gttcgatcaa ttccagtgcc cgttggcaga gggttatttc gtgcatatcg cctcccatta
    29341 actattgcca gctacaagca ataattgtgc cagtgttgat tatccctgcg gtgaataatg
    29401 tcgatgatgt cgaaatgaca cgtcgacacg gcgacgaaat tcatctttag cttaaaaatc
    29461 tctttaataa caataaatta aaagttggca cagaaaatgc ttaaagctgg catctctgtt
    29521 aaacgggtaa cctgacaatg actatttggg aaataagcga gaaagccgat tacatcgcac
    29581 agcggcatcg tcgcctacag gaccagtggc acatctactg taattcgctg gttcagggga
    29641 tcacgttatc gaaagcgcgc ctgcatcacg ccatgagctg cgcgccggac aaagaactct
    29701 gtttcgtcct ttttgaacat tttcgtattt atgtcaccct ggcggatggc tttaacagcc
    29761 acaccatcga gtattacgtc gaaacaaaag agggtgaaga caaacagtgg attgcgcagg
    29821 cgcaactgag cattgacggc atgattgatg gcaaggtcaa catccgcgat cgcgaacagg
    29881 ttctggaaca ctatctcgaa aaaatcgctg gcgtttacga cagcttatat accgccattg
    29941 aaaacaatgt gccggtgaat ttaagccaac tggtaaaggg acaaagcccg gcagcatgag
    30001 ctgaggcttt gcccgttttg caggcgttac gcctgtttgg ggatgggcgt gtcgatgagt
    30061 gtcgaaaatg acatttcatc ggcatgtttt cgtcaaaaat gacaatcacc tgaggaatgc
    30121 ctggtgaatc gttttgtaat tgctgactcc acgctctgta tcggctgcca cacttgtgag
    30181 gctgcctgtt cagagacgca tcgccagcac ggcctgcaat caatgccgcg cctgcgagtg
    30241 atgctcaatg aaaaagaatc tgcgccgcag ctctgtcacc actgtgaaga tgcgccctgt
    30301 gcggtggttt gcccggttaa cgccatcacc cgcgttgatg gggccgtgca gttgaatgaa
    30361 agtctgtgcg taagctgcaa actgtgcggc atcgcctgcc cgtttggcgc aattgaattt
    30421 tccggcagcc gtccgctgga tattccggca aacgccaata ccccgaaagc gccaccggca
    30481 ccgcctgctc cggcgcgtgt cagcacattg cttgactggg tgccaggtat tcgcgcgatc
    30541 gccgtcaaat gtgacctttg tagttttgat gaacaaggtc cggcctgcgt gcggatgtgc
    30601 ccgactaaag ccctgcatct ggtggataac accgatatcg cccgcgtcag caaacgtaag
    30661 cgtgagctga cttttaacac ggactttggc gatctcacct tgtttcagca ggctcaaagt
    30721 ggagaggcta aatgagcgca atttccctga tcaatagcgg cgtggcgtgg tttgtcgccg
    30781 ccgctgttct ggcatttctc ttttcttttc aaaaggcgct aagcggctgg atagccggaa
    30841 ttggcggcgc ggttggtagc ctgtatacgg cagccgcggg ctttactgtg ctgactggcg
    30901 cggttggcgt gagcggtgcg ctgtcgctgg taagctacga tgtgcaaatc tctccgctta
    30961 acgcgatttg gctgattacg ctcggtctgt gcggtctgtt tgtcagcctc tacaacattg
    31021 actggcatcg ccacgcgcag gtgaagtgca acggcttgca gatcaatatg ttgatggctg
    31081 ccgccgtctg cgccgtcatt gccagcaacc tcggcatgtt cgtggtaatg gccgaagtca
    31141 tggccttgtg tgcggtgttc ctcaccagca acagcaaaga gggcaaactg tggtttgcgc
    31201 tggggcgtct tggcactctg ctgctggcga ttgcttgctg gctgctgtgg cagcgttacg
    31261 gcacgctgga tctgcgcctg ctggatatgc gtatgcaaca gttgccgctc ggttccgata
    31321 tctggctgct cggtgtgatt ggctttggcc tgctggccgg gattattccg ctgcacggct
    31381 gggtgccgca ggcacatgct aacgcctctg cgccagctgc cgcgttgttt tccacggtag
    31441 tcatgaaaat tggcctgctg ggcattttaa ccctgtcact gctgggcggt aatgcaccgc
    31501 tgtggtgggg gatcgcactg ctggtactcg gcatgatcac cgcattcgtc ggcggtctgt
    31561 atgcgttgat ggaacataat atccaacgtc tgctggctta ccacaccctg gagaatatcg
    31621 gcattatcct gctggggctg ggcgctggcg taacgggtat cgcgctcgaa caaccggcgc
    31681 tgattgccct tggtctggtc ggtggtctgt accatctgct taaccatagc ctgttcaaaa
    31741 gcgtgctttt cctcggggcg gggagcgtct ggttccgtac cggtcatcgc gatatcgaaa
    31801 aactcggtgg tattggcaag aaaatgccgg ttatctccat cgccatgtta gtcgggctga
    31861 tggcaatggc tgcgctgccg ccactgaacg ggtttgccgg ggaatgggtt atctatcaat
    31921 ccttcttcaa actgagcaat agtggcgcgt ttgttgcccg tcttctcggg ccgctgcttg
    31981 ccgtggggct ggcaattacc ggtgcgctgg cggtgatgtg tatggcgaaa gtttatggcg
    32041 ttactttcct cggcgcgccg cgtaccaaag aggccgaaaa cgccacctgt gcgccgctcc
    32101 tgatgagcgt aagcgtagtg gcactggcga tttgctgcgt aattggcggt gttgctgcgc
    32161 cgtggctact gccgatgctc tctgctgctg tacctctgcc gctggagcct gctaacacca
    32221 ccgtttctca acccatgatc acgttgctgc tgattgcctg cccgctgctg ccattcatca
    32281 ttatggcgat ttgcaaaggc gatcgtttgc cgtcgcgttc ccgcggcgcg gcctgggtgt
    32341 gcggctacga tcatgaaaaa tcaatggtga ttaccgctca cggttttgcc atgccggtga
    32401 aacaggcgtt tgcgccggtg ctgaaactac gcaaatggct gaatccggtg tcgctggtgc
    32461 cgggctggca gtgcgagggg agtgcgttgc tgttccgccg gatggcgctg gttgaactgg
    32521 cggtgctggt ggtgattatt gtttcacgag gagcctgaga atgagtgttt tatatccgtt
    32581 aattcaggcg ctggtgttat ttgccgttgc gccgctgctc tccggtataa cccgcgtggc
    32641 gcgcgcccgc ttgcataacc gtcgcgggcc gggcgtgttg caggagtatc gcgacattat
    32701 caaactgctg ggtcgccaga gcgttggccc ggatgcctcc ggctgggtgt tccgcctgac
    32761 gccgtatgtg atggtgggcg tcatgctgac tatcgccact gcgctgccgg tggtgaccgt
    32821 cggttctccg ctgccgcaac tgggcgattt gatcacctta ctgtatctct tcgccatcgc
    32881 gcgtttcttc tttgccattt ctggtctgga taccggtagc ccgtttaccg ctatcggcgc
    32941 gagccgtgaa gcgatgcttg gcgtgttggt tgaaccgatg ctgctgcttg gtctgtgggt
    33001 cgccgcacag gttgccggtt ccaccaacat cagcaacatc accgacaccg tttatcactg
    33061 gccactgagc cagagcatcc cgctggtgct ggcgctttgt gcctgtgcgt tcgccacttt
    33121 tatcgaaatg ggcaaactgc cgttcgacct ggcggaagcc gagcaggagt tgcaggaagg
    33181 tccgctctct gagtacagcg gcagcggttt tggcgtcatg aaatggggca tcagcctgaa
    33241 acagctggtg gtgttgcaga tgttcgtcgg ggtgtttatt ccgtggggac aaatggaaac
    33301 cttcaccgtc ggtgggctgc tgctggcact ggtgattgcc atcgtcaaac tggtggtcgg
    33361 cgtactggtt atcgcgctgt tcgaaaacag catggcccgt ctgcgtcttg atattactcc
    33421 gcgcattacc tgggctgggt ttggctttgc atttttagcg ttcgtctcct tgctggcggc
    33481 gtgattaaag agagtttgag catgtctgaa gaaaaattag gtcaacatta tctcgccgcg
    33541 ctgaatgagg catttccggg cgtcgtgctg gaccacgcct ggcagaccaa agatcagcta
    33601 accgtcaccg tgaaggtgaa ctacctgccg gaagtggtgg agtttctcta ctacaaacaa
    33661 gggggctggc tgtcggtgct gtttggtaac gacgaacgca aactgaatgg tcattacgcc
    33721 gtttactacg tgctgtcgat ggagaagggc accaaatgct ggataaccgt gcgcgtcgaa
    33781 gttgacgcca acaaaccgga atatccgtcc gtgacaccgc gcgttccggc ggcggtgtgg
    33841 ggcgagcgcg aagtgcgcga tatgtacggt ttgattccgg tagggcttcc ggatgaacgt
    33901 cgtctggtgc tgccggatga ctggccggat gaactttatc cgctgcgtaa agacagcatg
    33961 gattatcgtc agcgtccggc accgactacc gatgctgaaa cctacgagtt catcaacgaa
    34021 ctgggcgaca agaaaaacaa tgtcgtgccg attggtccgc tgcacgtcac ttctgacgaa
    34081 ccgggtcact tccgtctgtt cgtcgatggc gaaaacatta tcgacgccga ctaccgcctg
    34141 ttctatgtcc atcgcggcat ggaaaaactg gcggaaaccc gcatgggtta caacgaagtg
    34201 accttcctct ctgaccgtgt gtgcgggatc tgcggctttg ctcacagcac cgcctacacc
    34261 acgtcggtgg aaaacgcgat gggtattcag gtgccagaac gtgcgcagat gatccgcgcc
    34321 attctgctgg aggtggaacg cctgcactcg catctgctca acctcggcct ggcctgtcac
    34381 tttaccggct tcgactccgg ctttatgcag ttcttccgcg tgcgtgaaac ctccatgaaa
    34441 atggcagaga tccttaccgg tgcacgtaaa acctacggcc tgaacctgat cggcgggatt
    34501 cgtcgcgatc tgctgaaaga cgacatgatc cagacccgcc agctggcaca acagatgcgt
    34561 cgtgaagtgc aggagctggt ggatgtgctg ctgagcacac cgaacatgga acaacgcacc
    34621 gtcggcattg gtcgtctgga cccggaaatc gctcgcgact tcagtaacgt tggcccgatg
    34681 gtccgtgcca gcggtcatgc ccgtgatacc cgtgccgatc acccgtttgt tggttatggc
    34741 ctgctgccaa tggaagtcca cagcgagcag ggctgcgacg ttatttcccg tctgaaagtg
    34801 cgtatcaacg aagtctatac cgcgctgaat atgatcgatt acggtctgga taacctgccg
    34861 ggcggcccgc tgatggtgga aggctttacc tacattccgc accgtttcgc gctgggcttt
    34921 gccgaagcgc cgcgcggcga cgatatccac tggagcatga ccggcgacaa ccagaagctg
    34981 taccgctggc gctgccgtgc ggcgacctac gcaaactggc cgaccctgcg ctacatgctg
    35041 cgcggcaaca ccgtttccga tgcgccgttg attatcggta gcctggaccc ttgctactcc
    35101 tgtaccgacc gcatgaccgt ggtcgatgta cgtaagaaga agagcaaagt ggtgccgtac
    35161 aaagaactcg agcgctacag cattgagcgt aaaaactcgc cgctgaaata aggaatcgcc
    35221 atgtttacct ttatcaaaaa agtcatcaaa accggcacgg cgacctcgtc ttatccgctg
    35281 gagccgattg cggttgataa aaacttccgt ggtaagccag agcagaaccc gcagcagtgc
    35341 atcggctgct cggcctgcgt caatgcctgc ccgtcaaacg ccttaacggt tgaaactgac
    35401 ctcgccacag gagagcttgc ctgggagttt aatctcgggc gctgcatctt ctgtggacgc
    35461 tgcgaagaag tttgcccgac ggcggcgatc aaactgtcgc aagagtacga actggcggtg
    35521 tggaagaaag aagacttcct gcaacagtcc cgcttcgcgc tgtgcaactg tcgcgtctgc
    35581 aatcgtcctt tcgccgtcca gaaagagatc gactacgcca ttgcgctgct taagcacaac
    35641 ggcgacagcc gcgcggaaaa ccaccgcgaa agctttgaga cttgcccgga atgtaagcgc
    35701 cagaaatgcc tggtgccgtc cgaccgtatt gaactgactc gccatatgaa agaggccatc
    35761 tgatgagcaa tttattaggc ccccgtgacg ctaacggcat tccggtcccg atgacggtgg
    35821 atgaatccat cgccagcatg aaggcgtcgt tactgaaaaa aatcaaacgt tctgcctacg
    35881 tttaccgcgt ggactgcggc ggctgcaacg gctgtgaaat cgaaattttc gccacgcttt
    35941 cgccgctgtt cgatgcagaa cgcttcggca ttaaagtcgt cccgtcaccg cgtcatgcgg
    36001 atattttact gtttaccggc gcggtcaccc gtgcaatgcg atcccctgcg ctgcgtgcgt
    36061 ggcagtccgc gccggacccg aaaatctgta tctcctacgg tgcctgcggt aacagcggcg
    36121 ggatcttcca cgatctctac tgcgtgtggg gcggtacgga taaaatcgtc ccggtagatg
    36181 tctacatccc tggctgcccg ccaacgccag ccgctacgct atacggcttt gcaatggcgc
    36241 tcggcctgct ggagcagaaa attcacgccc gtggcccggg tgagcaggat gaacaaccgg
    36301 cggaaatcct tcatggcgat atggtgcagc cgctgcgtgt gaaagtggat cgcgaagcac
    36361 gccgcctggc gggttatcgt tacggtcgcc agattgccga tgatttcctg acgcaattag
    36421 ggcagggcga agaacaggtt gcacgctggc tggaagcgga aaacgatccg cgtctgaacg
    36481 agattgtcag ccatctgaat catgttgttg aagaggcgcg tatccgatga gtgaaaaggt
    36541 ggtgttcagt caactgagcc gtaaatttat tgatgagaac gatgccacgc ccgccgaggc
    36601 gcagcaggtg gtctattaca gcctggcgat tggtcaccac cttggggtta tcgattgcct
    36661 ggaagcggcg ctcacctgcc cgtgggatga ctatctggca tggattgcca ctctggaggc
    36721 gggcagcgaa gcccgccgta aaatggaagg cgtgccgaag tacggtgaga tcgtcatcga
    36781 cattaaccat gtgccgatgc tggctaacgc attcgataaa gcccgggcag cgcaaacttc
    36841 gcagcagaaa gaatggagta ccatgctgtt aagtatgctg catgatattt atcaggaaaa
    36901 tgccatctat ttgatggtga ggagattgcg tgactgacgt tttactctgt gttggcaata
    36961 gcatgatggg cgatgatggc gcaggcccgc tgctggcgga aaagtgcgcc gccgcgccga
    37021 aaggtaactg ggtggtgatt gacggcggta gcgcaccgga aaacgatatc gtcgccatcc
    37081 gtgaactgcg cccgacacga ctgctgattg tcgacgccac ggatatgggg ctaaaccccg
    37141 gcgagatccg catcatcgac ccggatgaca tcgccgagat gtttatgatg actacccata
    37201 acatgccgct gaactacctt atcgaccagt tgaaagaaga cattggcgaa gtgattttcc
    37261 tcggcatcca gccggatatc gtcggctttt actacccgat gacccagccg attaaagatg
    37321 cggtggaaac cgtttatcaa cgtctggaag gctgggaagg gaatggcggc ttcgcgcagt
    37381 tagcggtgga agaagagtag tttttcagta aggaattcag ggaattgaac atcagaacag
    37441 ggatgtgcgc ttttttattg agcttagtgc tgcctgcaca ggccaccagc tttaccgaat
    37501 atctgccgat gagtgacagc gaatacgcgc aaaagcgggc gcttaaacct ctcctgacca
    37561 tgccttatga tgccgagcaa aactggcatt ttagaaaagt gggcgttgct ggcgtgacgc
    37621 ttgagaagat gccaaatgaa gaggattact ggcaattaac tgctaaagac cgcaccggta
    37681 aatcctggaa ggtgcctgtg ggtttgctgg caaatatggc gggcaaaggg cagttctatc
    37741 gtgccgatct ggatcgtaac ggcattcagg atctggttat ctggttacca agtgctggta
    37801 atggtctggc tccttatgca catttaatcc ttatgacgtt cacgcgcgat ggtcgtccct
    37861 gtgtctttga accttgggga ttctacaccg caagcaaaac tggcgtagat gatttgctgg
    37921 atttgcaagg caatggccac acgcaattgc tggatatgca gtttgattcc ggttactgga
    37981 taaccagtct gtatcaggta aaagacgcca aatggcagcg tgttcacggc tggtttggca
    38041 agttaagcta tcccgcgctc acgcgtttta cttatacccc caaccgtaaa ctcgtcctca
    38101 aacccatcgc cgggcgcgac ccgcaaacgg aagatctggc actgacgcag cgttgcttga
    38161 taaaggggga tgttcttgat ggggtgaacc agctctgatg ccaaacgcca gattgcccga
    38221 tgcgcaacgc ttatcgggcc ttgtaggccg gataaggcgt tcacgccgca tccggcaatg
    38281 ttacttactc taaatcctca ccattactgg caatcacttt tttataccac cagaacgatt
    38341 tcttacgcgt gcgcgccagc gtgccgttgc ctgcatcgtc acggtctaca tagacaaaac
    38401 cgtagcgttt gctcatttca cccgttgagg cggaaaccaa atcaatacag ccccaggtgg
    38461 tgtagcccat cagcggaatg ccatctgcaa tcgcttcgcc cattgcgcgg atatgttcgc
    38521 gtaaatagct gatgcgatag tcatcgttaa tctcaccgtt ggcagcaaat tcatcttttg
    38581 cgccgaggcc gttttccacc agaaacagcg gcttctgata gcggtcgtac atcatgttca
    38641 tggtgatacg cagaccgagt ggatcaatac cccagcccca gtcgcttacc tgtagatacg
    38701 ggttacgcag cgatttcact acattcgccg cactgctgtt gttggcgttc atctccgccg
    38761 aggcgcagcg cgaggcgtaa tagctgaatg agacaaaatc gacggtgttt ttcaggattt
    38821 catcatcgcc cggtgctttg ttgatggtta cccctttttc gcggaatacg cgggcagagt
    38881 aagccggata cgcgccccgc gcctgcacat cgataaaaaa caggttttcc cggtctttct
    38941 ccaacgccgc ccagacatcc tccggcttgc aactgtaagg gtagaagttg ccgcccgcca
    39001 gcatacagcc cacctgattt tgcgggttaa cctcatgggc gattttggta gctagcgcac
    39061 tggcaaccag ctggtgatgc gcggcctgat atttcacctg atcctgattt tccccttctt
    39121 caaacaccag acccgcgccg gagaacgggc tatgcaacat aatgttgatt tcattgaagg
    39181 taagccagta tttcaccaga ccatcaaatg cttcaaagca ggtccgggcg tagcggctga
    39241 aaaactccac cagcttgcgg ttacgccagg agccatattc ggtgaccaga tgcatcggca
    39301 catcgaagtg gcacaacgtg accagcggtt cgataccgta ctttttacac tcttcaaata
    39361 ccgcgcgata aaaagcaatg ccctgctggt tgggcgtcag ttcatcgccc tgcgggaaga
    39421 ggcggctcca ggcaattgag gtacggaaca ctttgaatcc catctctgcc atcagggcga
    39481 tatcttcttt ataacgatga taaaaatccg tcgcctcatg gctgggataa aactcgtcat
    39541 cgcgcaactg aaaacgtttt tccagcccca gtttcaccgc cattcgatgc tcgccgtgtg
    39601 ggatcatatc gacggtggtc agccctttgc caccttcacg gaacgcacct tcagactggt
    39661 tggcggcaag cgcgccgccc cataaaaaac cttctggaaa tactgacatt ttcatcctca
    39721 attaagactt acttctttgg cgacggtcgg ttgtacactc tgatgctttc gcgcctgggc
    39781 agccgcttcc tcaacaggaa tatcctcaaa gccgagcaac agtgtgagga taaacgacag
    39841 caccaccgcc agcgccatga ccgcgaacac ccagacgatg ctcattggat tcgccggatc
    39901 gaagaactgc acgctggtaa atagccccgg cgctgccatt gagtggctgg caagccccgc
    39961 cataccggca accgcgccgc aaataaaacc actgataaga ctggcgataa gcggacgttt
    40021 caggcggatc gccacgccat ataacgccgg ttcggaaatc cccgccataa tggctgatgc
    40081 cgccgcagcc agcgccgtct ggcgcagttc cgggtttttc gttttccacg ccaccgccag
    40141 tgatgaaccg cccagcgaca ggttagcgcc gatctctgat ggcatgacca ttccttcttt
    40201 gccggtttcg gcaatggtct gaatgatggt tggcgtaaag acgcggtgca tcccggtcat
    40261 taccagcaga ggccacagcg cgcccataat ggcgactgaa agccagccca gataaccatg
    40321 aatggtgtaa accagcgccg aaatggcgct accgatccag ataccaatcg ggccaatcag
    40381 caggattgcc agcggtgcgg caatcaacac aatcagcatt ggcttgagga agtttttcgt
    40441 caccgccggt gtaatgctgt ccacccagcg ttcgatatat gacaggcacc aggtcatcac
    40501 cagcgccggg atcaccgtgt aggtgtattt caccgcggtg accggaatca gggcaaattc
    40561 gacatgttca ccctgggccg ctttcgccat cagttcaata aaactcggat gtaccagcac
    40621 acccgcgatg gcaatcgcca gcgacatatt ggttttaaat ttgatggcgg cagatgccgc
    40681 gaccattagc ggcaggaaga agaaagcacc gtcaccaatc acattcagaa tggttaaggt
    40741 cggcgatcct tttgtcagca cgccgctcat ttcgaggatc atcgccagca gtttgaccat
    40801 cgatccgccg ataatcgccg ggatcagcgg tgacatggtg ccgatcagcg catcgaggat
    40861 ccccgcacca atgcgacgta gcgtaagttt gggtttaccc acgggctgtg cgggctgcat
    40921 atctcccggc agcaggctga cgacttcctg aaaggcttgt gaaacggtat tgccgataat
    40981 cacctgacac tggttgtcac tacgtaccac gccgagcacg ccggagatgg ttttcaacgt
    41041 cggactgtcg ataagctggt catctttgat aacgaagcgc aaacgcgtca tacagtgcgt
    41101 gactgccgag atgttatcaa cgccgcccag tgccgctatc accgagcgtg ccagcgccgc
    41161 ataatttttg gccatcggat tttatcctgt tatcagtagg gtagatgtag ttgttatcag
    41221 tcgacgtgac ggaaaaatgc cgatgctgaa acggaattag gaaaccggtt tcacaaaatc
    41281 atgaatagtt gtcactaaat aagcaagaga gttaattgtt cattttttat atttgtgaaa
    41341 ccggtcacct gaatattttt cccgccgatt gcgcaaggac aatcggcaaa cggtgcagta
    41401 cactgcgttg attgcgtgta aagggatgaa aatgatgacg acgatgctgg aagtggcgaa
    41461 gcgcgccggg gtttcaaaag cgaccgtttc ccgcgtgctt tcaggtaatg gctacgtcag
    41521 ccaggagact aaagatcgcg tgtttcaggc ggtagaagag agcggttacc gcccaaattt
    41581 gctggcccgc aatctgtcgg cgaaaagtac ccagacgctg gggctggtag tgactaacac
    41641 gctttaccac ggcatttatt ttagtgaact actgtttcat gccgcgcgaa tggcggaaga
    41701 gaaagggcgg cagttgctat tggcagatgg taaacacagc gccgaagaag agcgccaggc
    41761 gattcagtat ctgctggatc tgcgctgcga cgcgatcatg atttatccgc gctttttaag
    41821 cgtggatgaa attgatgaca tcattgacgc gcacagtcag ccgataatgg tgcttaatcg
    41881 ccgcctgcgc aaaaatagca gccatagcgt ctggtgcgat cataaacaga ccagctttaa
    41941 cgccgtggca gagttgataa acgccgggca tcaggagatt gctttcctta ccggctcgat
    42001 ggattccccc accagcattg aacgtcttgc cgggtataaa gacgcgctgt cgcagcatgg
    42061 tattgcgctc aatgaaaaac ttatcgctaa cggtaaatgg acgcctgcca gcggggccga
    42121 aggggtagaa acgttgctcg aacgtggggc taaatttagc gtgttagttg ccagtaacga
    42181 cgatatggcg ataggtgcga tgaaagcgtt gcacgagcgc ggcgtagcgg tgccagagca
    42241 ggtgtcagtt atcggattcg atgatatcgc tattgccccc tacaccgttc cggcgctctc
    42301 cagcgtaaaa attccggtaa ctgagatgat tcaggaaatt attggacggc tgatttttat
    42361 gctcgatggt ggggatttct caccgccgaa aaccttcagc ggaaaactga tccgccgcgg
    42421 ctccctcatt gctctttcgc aataaacatt gtcatcacat gtcatcgtca ctagtgtcga
    42481 tgacatcgtt tttcctgcat tacagaattt aatttcttta aataacagta aattaaaaac
    42541 tggcatgatt tgtgaatgta tcggcgcatt aactgtcatt gctggagaat ttgatgaacc
    42601 gtttcatcat tgctgacgcg agtaaatgta ttggttgccg tacctgtgaa gtggcgtgcg
    42661 tggtttctca tcaggagaat caggactgtg catcgctgac cccggaaact tttttaccgc
    42721 gaatccatgt cattaaaggt gtgaacattt ctacggcaac agtctgccgt cagtgtgaag
    42781 atgcaccgtg tgctaacgtc tgcccgaatg gtgctatcag ccgtgataaa gggtttgttc
    42841 atgtgatgca ggaacgttgc attggttgca aaacctgcgt tgtggcttgc ccgtatggcg
    42901 cgatggaagt ggtggtacgt ccggtgattc gcaacagcgg cgcggggctg aatgtacggg
    42961 ctgacaaagc cgaagccaat aaatgcgacc tctgtaacca tcgtgaagac ggcccggcgt
    43021 gtatggcggc ttgcccgacc catgcgctga tttgtgtcga tcgtaataaa cttgaacaac
    43081 tgagcgcaga aaaacgtcgc cgtacggcgc tgatgttcta aaaatcttac agaactgttc
    43141 tggcatcaaa ggaattcccg gaaatattcg tagaatacga agcgggcagt atcgccgacc
    43201 tgtgactgcc ggatgcgacg ctgtcgtgtc cggcagggcg ggttacgaaa ttcatgatgg
    43261 cgggaataac tcaatggcaa aaaacatatc ttgcggtgtc caactgcgta ttcgtggcaa
    43321 agtgcagggc gtcggttttc gtccgtttgt ctggcaactg gcacagcaat taaatcttca
    43381 cggcgatgtc tgtaatgacg gcgatggcgt agaagtccgg ctgctggaag ccccggaaac
    43441 gtttcttgtt caattgcatc agcactgccc gccgctggcg cgtattgata gcgtcgagcg
    43501 tgagccgttt atctggtcac aactgcccac tgagttcact atccgccaga gcacaggcgg
    43561 caccatgaat acgcaaattg tcccggatgc cgccacttgc cctgcttgcc ttgccgaaat
    43621 gaataccccg ggcgagcggc gttatcgtta tccgtttatc aactgtaccc actgcggccc
    43681 gcgtttcacc attattcgcg cgatgcctta cgaccgcccg tttaccgtga tggcggcgtt
    43741 tccgctgtgt ccggcttgtg acaaagagta ccgcgacccg ctcgatcgtc gcttccacgc
    43801 ccagccggtg gcctgcccgg agtgtggccc acatcttgaa tgggtaagtc atggtgaaca
    43861 tgcagaacaa gaggcggcat tacaggcggc tatcgcacag ttaaaaatgg gcaacattgt
    43921 cgccatcaaa gggattggcg gatttcatct cgcctgcgat gcgcgtaaca gtaacgcggt
    43981 ggcgacacta cgggcacgta aacatcgccc ggcgaaaccg ctggcggtca tgttgccagt
    44041 ggctgacggt ttaccagacg ctgcgcgcca gttgctttcc acgcccgccg cgccgattgt
    44101 gctggtggat aaaaaataca tacctgagct ttgtgatgat atcgcccctg gccttaacga
    44161 agttggggtg atgttgcctg cgaacccgct ccagcatttg ctgttacagg aactgcaatg
    44221 cccgctggtg atgacctccg gcaacctgag cggtaaacca ccagctatca gcaacgaaca
    44281 ggcgctggcg gatttgcagg gcattgccga cggattcttg atacataacc gcgacatcgt
    44341 gcagcggatg gatgattcgg tggtgcgcga aagcggcgaa atgctgcgcc gttcgcgggg
    44401 gtatgtgccg gatgcgctgg ctttgcctcc gggctttaaa aatgttccgc ctgtgctgtg
    44461 tctcggcgcg gatctgaaaa ataccttctg cctggtgcgc ggtgaacaag cggtgttgag
    44521 tcagcatctg ggcgatttaa gtgacgatgg catccagatg cagtggcgcg aagcgttacg
    44581 cctgatgcaa aacatctacg attttacccc gcaatacgtt gtgcatgacg cacatccggg
    44641 ctatgtctcc agccagtggg cgcgcgaaat gaatctgccg acgcaaacgg tgctgcatca
    44701 tcatgcccac gcggcggcgt gtctggcaga acatcactgg ccactggagg gcggtgatgt
    44761 cattgctttg acgctcgacg gtatcggtat gggggagaac ggcgctttgt ggggcggcga
    44821 gtgtctgcgg gtgaactatc gcgaatgcca gcacctgggc ggcttgccag cggtggcgct
    44881 tgctgggggc gatttggctg caaagcagcc gtggcgtaac ctgctggcgc agtgcctgcg
    44941 ctttgtgccg gagtggcaga attaccctga aacggcaagt gtgcaacagc aaaactggag
    45001 cgtgctggcg cgggccattg agcgtggaat taacgcgccg ctggcgtcat cgtgtgggcg
    45061 tttgtttgat gctgtggcgg cggcactggg ctgtgcgcca gccacgttaa gttatgaagg
    45121 tgaagcggct tgtgctctgg aggcgctcgc agcttcatgc gacggagtga cgcatccggt
    45181 gacgatgccg ctggtggaca atcaactgga tctcgccact ttctggcagc agtggctgaa
    45241 ctggcaggca ccggttaatc aacgcgcgtg ggcgtttcat gatgcgctgg cgcagggttt
    45301 tgccgcgttg atgcgtgagc aggccacgat gcgcggtatc actacgctgg tatttagcgg
    45361 tggggttatt cataaccgat tgctgcgtac acgcctggcg cattatctcg ctgatttcac
    45421 attactcttt ccgcagagtt taccggcggg tgatggcggc ttgtctctgg ggcagggggt
    45481 tattgctgcg gcgcgcatcc tgcatgctgc atagtggcgg atgtggcgta actaagcgtt
    45541 aaaagtagcg cggacagtcc cctcgccctt tgggcgaggg agaaaaccat gcctacatcg
    45601 gcaacgtttt caacaatcca aacgcctctt tcatccgatc ctcactgacc acaaaggcgc
    45661 gaagctggtc agcatcgtca acaccgcgcg caaccattcc ctggcgttcg gtattaattt
    45721 gccagcgtaa atcctgacgc tgggtttcgc ctgccagatg cagcggtaat tctggcgttt
    45781 tgattttcac cagcatcgcc gggagtttca gcggcgtgtt attgccgaga agattttttg
    45841 ccagcaccat tgcgctaagt tgaatcggct ggaggaacgg caatacctga ccgttaattt
    45901 ccgcgcaatc gcccagcgca taaatatcgg cattactggt ttgcagataa ctatcgacgc
    45961 aaacgccacg attaatcgtc agcccggcgc gtcgtgccag ggcggtttcc gggcgcagtc
    46021 cggtggcggc aattaccgca tccacttcaa tgcagcgctg gcattccagc gttgccagaa
    46081 tgccagaatc cgttttttcc agtccctgca actgagattt taacagcaga tgaacgccca
    46141 tctccgtcaa ccgatgctgc aagcggctgc ttacttccgg tggcattaac gacgccagaa
    46201 tactggcagc gttgtcgatc agcgtgaccg ctttgcctgc ccgacaaaaa tccatcgcca
    46261 gctcgctacc aatcaagcca ccgccaacaa tcaacacgcg tcgggcatcc cgcagttgcg
    46321 tttcacaggc gcgatactct tgctgactat ttaacgtcag cattaactca cgcccgggca
    46381 ctggcgggac aaaggcgctg gcaccggttg ccagcactaa cttgtcgtat tgccactgat
    46441 tattctgact tttcaccaca tgggcttcgg catcgatatc cgttacccag gtgtgcggaa
    46501 acaggcgcag attaaactgc tcggcaaatt cacctgccgt ctggcgggta aggtcgtcgg
    46561 cacgttgccc ctgactgata acatggctga ggtcaggttt gttgtactca tccatgctgt
    46621 cggcggcaat cagagttaat ggaatactgg cgtcctgttt gcgaatattt ttcaccagtt
    46681 ggcgggcggc gaagcccgaa ccaatgatca caatgctgtt gctcattttg cctccgatgc
    46741 cagttcgtca aagacgtctt tgccgagaga gcattccggg cacaggaagt tatccgggac
    46801 ttcactccac ggcgttcctg gcgcaacgtc ctgcattggc tcaccttttg ccggatcgta
    46861 aatccactgg cagacgctgc attgcatccg tgggccgagg tcagccgtcg tggtggcaga
    46921 ggtttcttct ttaactaccg tattcaccgt gctctgcggc agcggcgcga gcgcccactg
    46981 acgggcgatt tcgcgaccgt gttcacgaca taactccaga gcgtcctggt ctggtcgcca
    47041 tttggctttc aggctaagcg acatttcgaa acccgcatcc tgcaggcgcg tggaaagacg
    47101 atccaccgca ccgccgctcc agccgtgaga gccgaaagcg ctggcgcgtt tgttacggaa
    47161 gcgtaatccg gtcatctcct ccaccagccc ggcgattttc ggcatcatca cattattcat
    47221 cgtagaggtg ccgaccagca tgccttttga gcggaagaca ttggtcagga tttcgttttt
    47281 atcgcttcgg gcgacgttga aaattttcac cgccacgcgt gggtcggttt ccgcaatccc
    47341 ctgggcgata gcgtcagcca tcatgcgggt gttattcgac atggtgtcgt agaaaatggt
    47401 gattctgtct tcctgataat ccgccgccca tttcaggtac agctcgacaa tttgcgtcgg
    47461 gttatcgcgc cataccacgc cgtgagaggt cgctatcata tcgactggca agttaaagcc
    47521 caggatctcg gtaattttcg gcgttaccag gcggctgaac ggcgtcagga tattggcgta
    47581 gtaacgctgg cactgctcga aaagctccgt ctgatccact tcatcgttga acagatgctc
    47641 gtcgcagtag tgctgaccga aagcatcgtt actgaacagc accgcgtcgc ctgtcaggta
    47701 agtcatcatg ctgtccggcc agtgcagcat tggcgtttcg acaaaaatga gctgtttgcc
    47761 gttgccgata tccagcgtgt cgccagtttt caccacatta aaattccact ccggatgatg
    47821 gtgatgacca tttatcgagt cgatagcgtt ggcagtacag tagatcggcg tatcgggaat
    47881 ttgtgccatc agttcggtca gcgccccggc gtggtcctct tcagcatggt taattacgat
    47941 gtaatcgata tctgccagat cgatttcatt acgcaggttc tgcacaaatt cgcggctgaa
    48001 tttatggtcg acggtgtcga tcagcacgtt tttttcttca cggatgaggt agctgttgta
    48061 gctgctgccg cgcagcgttt tatattctgt accgtgaaaa tcacgcactt cccagtcacg
    48121 ttgaccaacc caatgaatgt tatttttcac cacaatagac atagcaacct caatttattc
    48181 agcgtgttct aaaaagatgt cttgctaatt gcagattgcg tgccagtttt ttatctgatt
    48241 gatttttatg tataaattaa ttcatgctca ttaatgagta gtcaaaatga ctattaatca
    48301 aatggtcgat atgacaatat ctatagtcaa attgacagtg aggcaaagat gagtttttcc
    48361 gttgatgtgc tggcgaatat cgccatcgaa ttgcagcgtg ggattggtca tcaggatcgt
    48421 tttcagcgcc tgatcaccac gctgcgtcag gtgctggagt gtgatgcctc tgcgttgcta
    48481 cgttatgact cgcgg
//
LOCUS       DAMZFT010000018        47535 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.018, whole genome shotgun sequence.
ACCESSION   DAMZFT010000018 DAMZFT010000000
VERSION     DAMZFT010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 47535)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47535)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47535
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.018"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..105
                     /gene="rrf"
                     /locus_tag="PN977_001203"
     rRNA            <1..105
                     /gene="rrf"
                     /locus_tag="PN977_001203"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            430..1728
                     /gene="kgtP"
                     /locus_tag="PN977_001204"
     CDS             430..1728
                     /gene="kgtP"
                     /locus_tag="PN977_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoglutarate permease"
                     /protein_id="HDI6041259.1"
                     /translation="MAESTVTADSKLTSSDTRRRIWAIVGASSGNLVEWFDFYVYSFC
                     SLYFAHIFFPSGNTTTQLLQTAGVFAAGFLMRPIGGWLFGRIADKHGRKKSMLLSVCM
                     MCFGSLVIACLPGYETIGTWAPALLLLARLFQGLSVGGEYGTSATYMSEVAVEGRKGF
                     YASFQYVTLIGGQLLALLVVVVLQHTMEDAALREWGWRIPFALGAVLAVVALWLRRQL
                     DETSQQETRALKEAGSLKGLWRNRRAFIMVLGFTAAGSLCFYTFTTYMQKYLVNTAGM
                     HANVASGIMTAALFVFMLIQPLIGALSDKIGRRTSMLCFGSLAAIFTVPILSALQNVS
                     SPYAAFGLVMCALLIVSFYTSISGILKAEMFPAQVRALGVGLSYAVANAIFGGSAEYV
                     ALSLKSIGMETAFFWYVTLMAVVAFLVSLTLHRKGKGMRL"
     gene            complement(1725..2048)
                     /locus_tag="PN977_001205"
     CDS             complement(1725..2048)
                     /locus_tag="PN977_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiM family lipoprotein"
                     /protein_id="HDI6041260.1"
                     /translation="MRILFVCSLLLLSGCSHMANDSWSGQDKAQHFIASAMLSAAGNE
                     YAQHQGMSRDRSAMFGLMFSVSLGASKELWDSRPEGSGWSWKDFAWDIAGASTGYTVW
                     QLTRH"
     gene            complement(2094..3449)
                     /gene="pssA"
                     /locus_tag="PN977_001206"
     CDS             complement(2094..3449)
                     /gene="pssA"
                     /locus_tag="PN977_001206"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HDI6041261.1"
                     /translation="MLSKFKRNKHQQHLAQLPKISQSVDDVDFFYAPADFRETLLEKI
                     ASAKQRICIVALYLEQDDGGKGILNALYEAKRQRPELDVRVLVDWHRAQRGRIGAAAS
                     NTNADWYCRMAQENPGVDVPVYGVPINTREALGVLHFKGFIIDDSVLYSGASLNDVYL
                     HQHDKYRYDRYHLIRNRKMSDIMFEWVTQNIMNGRGVNRLDDVNRPKSPEIKNDIRLF
                     RQELRDAAYHFQGDADNDQLSVTPLVGLGKSSLLNKTIFHLMPCAEQKLTICTPYFNL
                     PAILVRNIIQLLREGKKVEIIVGDKTANDFYIPEDEPFKIIGALPYLYEINLRRFLSR
                     LQYYVNTDQLVVRLWKDDDNTYHLKGMWVDDKWMLITGNNLNPRAWRLDLENAILIHD
                     PQLELAPQREKELELIREHTTIVKHYRDLQSIADYPVKVRKLIRRLRRIRIDRLISRI
                     L"
     gene            complement(3563..6223)
                     /gene="pat"
                     /locus_tag="PN977_001207"
     CDS             complement(3563..6223)
                     /gene="pat"
                     /locus_tag="PN977_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein lysine acetyltransferase"
                     /protein_id="HDI6041262.1"
                     /translation="MSQRGLEALLRPKSIAVIGASMKPNRAGYLMMRNLLAGGFNGPV
                     LPVTPAWKAVLGVLAWPDIASLPFTPDLAVLCTNASRNLALLEELGEKGCKTCIILSA
                     PSSQHEDLRACALRHNMRLLGPNSLGLLAPWQGLNASFSPVPIKRGKLAFISQSAAVS
                     NTILDWAQQREMGFSYFIALGDSLDIDVDELLDYLARDSKTSAILLYLEQLSDARRFV
                     SAARSASRNKPILVIKSGRSPAAQRLLNTTAGMDPAWDAAIQRAGLLRVQDTHELFSA
                     VETLSHMRPLRGDRLMIISNGAAPAALALDALWSRNGKLATLSEETCQKLRDALPEHV
                     AISNPLDLRDDASSEHYIKTLDILLHSQDFDALMVIHSPSAAAPATESAQALIEAVKH
                     HPRSKYVSLLTNWCGEHSSQEARRLFSEAGLPTYRTPEGTITAFMHMVEYRRNQKQLR
                     ETPALPSNLTSNTAEAHLLLQQAIAEGATSLDTHEVQPILQAYGMNTLPTWIASDSTE
                     AVHIAEQIGYPVALKLRSPDIPHKSEVQGVMLYLRTANEVQQAANAIFDRVKMAWPQA
                     RIHGLLVQSMANRAGAQELRVVVEHDPVFGPLIMLGEGGVEWRPEDQAVVALPPLNMN
                     LARYLVIQGIKSKKIRARSALRPLDVAGLSQLLVQVSNLIVDCPEIQRLDIHPLLASG
                     SEFTALDVTLDIAPFEGDNESRLAVRPYPHQLEEWVELKNGERCLFRPILPEDEPQLQ
                     QFISRVTKEDLYYRYFSEINEFTHEDLANMTQIDYDREMAFVAVRRIDQTEEILGVTR
                     AISDPDNIDAEFAVLVRSDLKGLGLGRRLMEKLITYTRDHGLQRLNGITMPNNRGMVA
                     LARKLGFNVDIQLEEGIVGLTLNLAQREES"
     gene            complement(6255..6953)
                     /gene="tapT"
                     /locus_tag="PN977_001208"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
     CDS             complement(6255..6953)
                     /gene="tapT"
                     /locus_tag="PN977_001208"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
                     /EC_number="2.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-uridine aminocarboxypropyltransferase"
                     /protein_id="HDI6041263.1"
                     /translation="MTENAVLQLRAERIARATRPFLARGNRVRRCQRCLLPEKLCLCS
                     TITPAQAKSRFCLLMFDTEPMKPSNTGRLIADILPDTVAFQWSRTEPSLDLLDLVQNP
                     DYQPMVVFPASYADEQREVIFTPPAGKPPLFIMLDGTWPEARKMFRKSPYLDNLPVIS
                     VDLSRLSAYRLREAQAEGQYCTAEVAIALLDMVGDTGAAAGLGEHFTRFKTRYLAGKT
                     QHLGSITAEQLESV"
     gene            complement(7022..7441)
                     /gene="trxC"
                     /locus_tag="PN977_001209"
     CDS             complement(7022..7441)
                     /gene="trxC"
                     /locus_tag="PN977_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxC"
                     /protein_id="HDI6041264.1"
                     /translation="MNTVCTHCQAINRIPDDRIEDAAKCGRCGHDLFDGEVINATGET
                     LDKLLKDDLPVVIDFWAPWCGPCRNFAPIFEDVAQERSGKVRFVKVNTEAERELSSRF
                     GIRSIPTIMIFKNGQVVDMLNGAVPKAPFDSWLNESL"
     gene            7648..8685
                     /locus_tag="PN977_001210"
     CDS             7648..8685
                     /locus_tag="PN977_001210"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HDI6041265.1"
                     /translation="MNDEMKGKSGKVKVMYVRSDDDSDKRTHNPRTGKGGGRPGKSRA
                     DGGRRPARDDKQSQPRDRKWEDSPWRTVSRAPGDETPEKADHGGISGKSFIDPEVLRR
                     QRAEETRVYGENACQALFQSRPEAIVRAWFIQSVTPRFKEALRWMAANRKAYHVVDEA
                     ELTKASGTEHHGGVCFLIKKRNGTTVQQWVSQAGAQDCVLALENESNPHNLGGMMRSC
                     AHFGVKGVVVQDAALLESGAAIRTAEGGAEHVQPITGDNIVNVLDDFRQAGYTVVTTS
                     SEQGKPLFKTSLPAKMVLVLGQEYEGLPDAARDPNDLRVKIDGTGNVAGLNISVATGV
                     LLGEWWRQNKA"
     gene            complement(8733..9422)
                     /gene="ung"
                     /locus_tag="PN977_001211"
     CDS             complement(8733..9422)
                     /gene="ung"
                     /locus_tag="PN977_001211"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HDI6041266.1"
                     /translation="MANELTWHDVLAEEKQQPYFLNTLQTVASERQSGVTIYPPQKDV
                     FNAFRFTELGDVKVVILGQDPYHGPGQAHGLAFSVRPGIATPPSLLNMYKELENTIPG
                     FTRPNHGYLESWARQGVLLLNTVLTVRAGQAHSHASLGWETFTDKVISLINQHREGVV
                     FLLWGSHAQKKGAIIDKQRHHVLKAPHPSPLSAHRGFFGCNHFVLANQWLEQRGETPI
                     DWMPVLPAESE"
     gene            9727..10110
                     /gene="grcA"
                     /locus_tag="PN977_001212"
     CDS             9727..10110
                     /gene="grcA"
                     /locus_tag="PN977_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007728348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autonomous glycyl radical cofactor GrcA"
                     /protein_id="HDI6041267.1"
                     /translation="MITGIQITKAANDDLLNSFWLLDSEKGEARCIVAKAGFAEDEVV
                     AVSKLGDIEYREVPVEVKPEVRVEGGQHLNVNVLRRETLEDAVKHPEKYPQLTIRVSG
                     YAVRFNSLTPEQQRDVIARTFTESL"
     gene            complement(10165..10752)
                     /gene="eamB"
                     /locus_tag="PN977_001213"
     CDS             complement(10165..10752)
                     /gene="eamB"
                     /locus_tag="PN977_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/O-acetylserine transporter"
                     /protein_id="HDI6041268.1"
                     /translation="MTPTLLSAFWTYTLITAMTPGPNNILALSSATSHGFRQSTRVLA
                     GMSLGFLIVMLLCAGISFSLAVIDPAVVHLLSWAGAAYIVWLAWKIATSPTKEDGLQT
                     KPISFWASFALQFVNVKIILYGITALSTFVLPQTQALSWVIGVSVLLAMIGTFGNVCW
                     ALAGHLFQRLFRQYGRQLNIVLALLLVYCAVRIFY"
     gene            10855..11736
                     /locus_tag="PN977_001214"
     CDS             10855..11736
                     /locus_tag="PN977_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311470.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDI6041269.1"
                     /translation="MDLRRFITLKTVVEEGSFLRASQKLCCTQSTVTFHIQQLEQEFS
                     VQLFEKIGRRMCLTREGKKLLPHIYELTRVMDTLREAAKKESDPDGELRVVSGETLLS
                     YRMPQVLQRFRQRAPKVCLSLQSLNCYVIRDALLNDEADVGVFYRVGNDDALNRRELG
                     EQSLVLVASPQIADVDFTEPGRHNACSFIINEPQCVFRQIFESTLRQRRITVENTIEL
                     ISIESIKRCVAANIGVSYLPRFTVANELASGELIELPFGEQSQTITAMCAHHAGKAVS
                     PAMHIFIQCVEECFLPG"
     gene            complement(11769..13103)
                     /gene="srmB"
                     /locus_tag="PN977_001215"
     CDS             complement(11769..13103)
                     /gene="srmB"
                     /locus_tag="PN977_001215"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase SrmB"
                     /protein_id="HDI6041270.1"
                     /translation="MTVTTFSELELDESLLEALQDKGFTRPTAIQAAAIPPALDGRDV
                     LGSAPTGTGKTAAYLLPALQHLLDFPRKKSGPPRILILTPTRELAMQVADHARELAKH
                     THLDIATITGGVAYMNHAEVFSENQDIVVATTGRLLQYIKEENFDCRAVETLILDEAD
                     RMLDMGFAQDIEHIAGETRWRKQTLLFSATLEGDAIQDFAERLLEDPVEVSANPSTRE
                     RKKIHQWYYRADDLEHKTALLVHLLKQPEATRSIVFVRKRERVHELANWLREAGINNC
                     YLEGEMVQGKRNEAIKRLTEGRVNVLVATDVAARGIDIPDVSHVFNFDMPRSGDTYLH
                     RIGRTARAGRKGTAISLVEAHDHLLLGKVGRYIEEPIKARVIDELRPKTRAPSEKQTG
                     KPSKKVLAKRAEKKKAKEKEKPRVKKRHRDTKNIGKRRKPSGTGVPPQTTEE"
     gene            13235..13972
                     /gene="trmN"
                     /locus_tag="PN977_001216"
     CDS             13235..13972
                     /gene="trmN"
                     /locus_tag="PN977_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(1)(Val)
                     (adenine(37)-N(6))-methyltransferase TrmN"
                     /protein_id="HDI6041271.1"
                     /translation="MSQSTSVLRRNGFTFKQFFVAHDRCAMKVGTDGILLGAWAPVAG
                     VKRCLDIGAGSGLLALMLAQRTDDSVMIDAVELESEAAAQAQENINQSPWAERINVHT
                     ADIQQWITQQTVRFDLIISNPPYYQQGVECSTPQREQARYTTTLDHPSLLTCAAECIT
                     EEGFFCVVLPEQIGNGFTELALSMGWHLRLRTDVAENEARLPHRVLLAFSPQAGECFS
                     DRLVIRGPDQNYSEAYTALTQAFYLFM"
     gene            complement(13957..15579)
                     /gene="nadB"
                     /locus_tag="PN977_001217"
     CDS             complement(13957..15579)
                     /gene="nadB"
                     /locus_tag="PN977_001217"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HDI6041272.1"
                     /translation="MNTLPEHSCDVLIIGSGAAGLSLALRLADQHQVIVLSKGPVTEG
                     STFYAQGGIAAVFDETDSIDSHVEDTLIAGAGICDRHAVEFVASNARSCVQWLIDQGV
                     LFDTHIQPNGAESYHLTREGGHSHRRILHAADATGREVETTLVSKALNHPNIRVLERS
                     NAVDLIVSDKIGLPGTRRVVGAWVWNRNKETVETCHAKAVVLATGGASKVYQYTTNPD
                     ISSGDGIAMAWRAGCRVANLEFNQFHPTALYHPQARNFLLTEALRGEGAYLKRPDGTR
                     FMPDFDERGELAPRDIVARAIDHEMKRLGADCMFLDISHKPADFIRQHFPMIYEKLLG
                     LGIDLTKGPVPIVPAAHYTCGGVMVDDHGRTDVEGLYAIGEVSYTGLHGANRMASNSL
                     LECLVYGWSAAEDITRRMPYAHDVSTLPPWDESRVENPDERVVIQHNWHELRLFMWDY
                     VGIVRTTKRLERALRRITMLQQEIDEYYAHFRVSNNLLELRNLVQVAELIVRCAMMRK
                     ESRGLHFTLDYPELLTHSGPSILSPGNHYINR"
     gene            15835..15990
                     /gene="rseD"
                     /locus_tag="PN977_001218"
     CDS             15835..15990
                     /gene="rseD"
                     /locus_tag="PN977_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rpoE leader peptide RseD"
                     /protein_id="HDI6041273.1"
                     /translation="MIRLQHDKQKQMRYGTLQKRDTLTLCLLKLQLMEWRFDSAWKFG
                     LGRLYLG"
     gene            15987..16562
                     /gene="rpoE"
                     /locus_tag="PN977_001219"
     CDS             15987..16562
                     /gene="rpoE"
                     /locus_tag="PN977_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005070454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoE"
                     /protein_id="HDI6041274.1"
                     /translation="MSEQLTDQVLVERVQKGDQKAFNLLVVRYQHKVASLVSRYVPSG
                     DVPDVVQEAFIKAYRALDSFRGDSAFYTWLYRIAVNTAKNYLVAQGRRPPSSDVDAIE
                     AENFESGGALKEISNPENLMLSEELRQIVFRTIESLPEDLRMAITLRELDGLSYEEIA
                     AIMDCPVGTVRSRIFRAREAIDNKVQPLIRR"
     gene            16595..17245
                     /gene="rseA"
                     /locus_tag="PN977_001220"
     CDS             16595..17245
                     /gene="rseA"
                     /locus_tag="PN977_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma-E factor RseA"
                     /protein_id="HDI6041275.1"
                     /translation="MQKEQLSALMDGETLDSELLNELAHNPEMQKTWESYHLIRDSMR
                     GDTPEVLHFDISSRVMAAIEEEPVRQPATLIPEAQPAPHQWQKMPFWQKVRPWAAQLT
                     QMGVAACVSLAVIVGVQHYNGQSETSQQPETPVFNTLPMMGKASPVSLGVPSEATANN
                     GQQQQVQEQRRRINAMLQDYELQRRLHSEQLQFEQAQTQQAAVQVPGIQTLGTQSQ"
     gene            17245..18201
                     /gene="rseB"
                     /locus_tag="PN977_001221"
     CDS             17245..18201
                     /gene="rseB"
                     /locus_tag="PN977_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E factor regulatory protein RseB"
                     /protein_id="HDI6041276.1"
                     /translation="MKQLWFAMSLVTGSLLFSANASATPASGALLQQMNLASQSLNYE
                     LSFISINKQGVESLRYRHARLDNRPLAQLLQMDGPRREVVQRGNEISYFEPGLEPFTL
                     NGDYIVDSLPSLIYTDFKRLSPYYDFISVGRTRIADRLCEVIRVVARDGTRYSYIVWM
                     DTESKLPMRVDLLDRDGETLEQFRVIAFNVNQDISSSMQTLAKANLPPLLSVPVGEKA
                     KFSWTPTWLPQGFSEVSSSRRPLPTMDNMPIESRLYSDGLFSFSVNVNRATPSSTDQM
                     LRTGRRTVSTSVRDNAEITIVGELPPQTAKRIAENIKFGAAQ"
     gene            18198..18677
                     /gene="rseC"
                     /locus_tag="PN977_001222"
     CDS             18198..18677
                     /gene="rseC"
                     /locus_tag="PN977_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR-reducing system protein RseC"
                     /protein_id="HDI6041277.1"
                     /translation="MIKEWATVVSWENGQALVSCDVKASCSSCASRAGCGSRVLNKLG
                     PQTTHTIVVPCDEPLVPGQKVELGIAEGSLLSSALLVYMSPLVGLFLIASLFQLLFAS
                     DIAALCGAILGGVGGFLIARGCSRKFAARSEWQPIILSVALPPGLVRFETSSEDASQ"
     gene            18874..20673
                     /gene="lepA"
                     /locus_tag="PN977_001223"
     CDS             18874..20673
                     /gene="lepA"
                     /locus_tag="PN977_001223"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HDI6041278.1"
                     /translation="MKNIRNFSIIAHIDHGKSTLSDRIIQICGGLSDREMEAQVLDSM
                     DLERERGITIKAQSVTLDYKASDGETYQLNFIDTPGHVDFSYEVSRSLAACEGALLVV
                     DAGQGVEAQTLANCYTAMEMDLEVVPVLNKIDLPAADPERVAEEIEDIVGIDATDAVR
                     CSAKTGVGVQDVLERLVRDIPPPEGDPEGPLQALIIDSWFDNYLGVVSLIRIKNGTLR
                     KGDKVKVMSTGQTYNADRLGIFTPKQVDRTELKCGEVGWLVCAIKDIHGAPVGDTLTL
                     ARNPAEKALPGFKKVKPQVYAGLFPVSSDDYEAFRDALGKLSLNDASLFYEPESSSAL
                     GFGFRCGFLGLLHMEIIQERLEREYDLDLITTAPTVVYEVETTSREVIYVDSPSKLPA
                     VNNIYELREPIAECHMLLPQAYLGNVITLCVEKRGVQTNMVYHGNQVALTYEIPMAEV
                     VLDFFDRLKSTSRGYASLDYNFKRFQASDMVRVDVLINGERVDALALITHRDNSQNRG
                     RELVEKMKDLIPRQQFDIAIQAAIGTHIIARSTVKQLRKNVLAKCYGGDISRKKKLLQ
                     KQKEGKKRMKQIGNVELPQEAFLAILHVGKDNK"
     gene            20689..21663
                     /gene="lepB"
                     /locus_tag="PN977_001224"
     CDS             20689..21663
                     /gene="lepB"
                     /locus_tag="PN977_001224"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HDI6041279.1"
                     /translation="MANMFALILVIATLVTGILWCVDKFFFAPKRRERQAAAQAAAGD
                     SLDKATLKKVAPKPGWLETGASVFPVLAIVLIVRSFIYEPFQIPSGSMMPTLLIGDFI
                     LVEKFAYGIKDPIYQKTLIETGHPKRGDIVVFKYPEDPKLDYIKRAVGLPGDKVTYDP
                     VSKELTIQPGCSSGQACENALPVTYSNVEPSDFVQTFSRRNGGEATSGFFEVPKNETK
                     ENGIRLSESKETLGDVTHRILTVPIAQDQVGMYYQQPGQQLATWIVPPGQYFMMGDNR
                     DNSADSRYWGFVPEANLVGRATAIWMSFDKQEGEWPTGVRLSRIGGIH"
     gene            21936..22616
                     /gene="rnc"
                     /locus_tag="PN977_001225"
     CDS             21936..22616
                     /gene="rnc"
                     /locus_tag="PN977_001225"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004248457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HDI6041280.1"
                     /translation="MNPIVINRLQRKLGYTFNHQELLQQALTHRSASSKHNERLEFLG
                     DSILSYVIANALYHRFPRVDEGDMSRMRATLVRGNTLAELAREFELGECLRLGPGELK
                     SGGFRRESILADTVEALIGGVFLDSDIQTVEKLILNWYQTRLDEISPGDKQKDPKTRL
                     QEYLQGRHLPLPTYLVVQVRGEAHDQEFTIHCQVSGLSEPVVGTGSSRRKAEQAAAEQ
                     ALKKLELE"
     gene            22613..23518
                     /gene="era"
                     /locus_tag="PN977_001226"
     CDS             22613..23518
                     /gene="era"
                     /locus_tag="PN977_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HDI6041281.1"
                     /translation="MSIDKSYCGFIAIVGRPNVGKSTLLNKLLGQKISITSRKAQTTR
                     HRIVGIHTEGAYQAIYVDTPGLHMEEKRAINRLMNKAASSSIGDVELVIFVVEGTRWT
                     PDDEMVLNKLRDGKAPVILAVNKVDNVQEKADLLPHLQFLASQMNFLDIVPISAETGL
                     NVDTIAAIVRKHLPEATHHFPEDYITDRSQRFMASEIIREKLMRFLGAELPYSVTVEI
                     ERFVSNERGGYDINGLILVEREGQKKMVIGNKGAKIKTIGIEARKDMQEMFEAPVHLE
                     LWVKVKSGWADDERALRSLGYVDDL"
     gene            23530..24258
                     /gene="recO"
                     /locus_tag="PN977_001227"
     CDS             23530..24258
                     /gene="recO"
                     /locus_tag="PN977_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HDI6041282.1"
                     /translation="MEGWQRAFVLHSRPWSETSLMLDVFTEESGRVRLVAKGARSKRS
                     TLKGALQPFTPLLLRFGGRGEVKTLRSAEAVSLALPLSGITLYSGLYINELLSRVLEY
                     ETRFSELFFDYLHCIQSLAGATGTPEPALRRFELALLGHLGYGVNFTHCAGSGEPVDD
                     TMTYRYREEKGFIASVVIDNKTFTGRQLKALNAREFPDADTLRAAKRFTRMALKPYLG
                     GKPLKSRELFRQFMPKRTVKTHYE"
     gene            24270..25001
                     /gene="pdxJ"
                     /locus_tag="PN977_001228"
     CDS             24270..25001
                     /gene="pdxJ"
                     /locus_tag="PN977_001228"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HDI6041283.1"
                     /translation="MAELLLGVNIDHIATLRNARGTAYPDPVQAAFIAEQAGADGITV
                     HLREDRRHITDRDVRILRQTLDTRMNLEMAVTEEMLAIAVETKPHFCCLVPEKRQEVT
                     TEGGLDVAGQREKMRDACKRLAEAGIQVSLFIDADEEQIKAAAEVGAPFIEIHTGCYA
                     DAKTDAEQAQELARIAKAATFAASLGLKVNAGHGLTYHNVKAIAAIPEMHELNIGHAI
                     IGRAVMTGLKDAVAEMKRLMLEARG"
     gene            25001..25381
                     /locus_tag="PN977_001229"
     CDS             25001..25381
                     /locus_tag="PN977_001229"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HDI6041284.1"
                     /translation="MAILGLGTDIVEIARIESVIARSGERLARRVLSDNEWAIWKTHH
                     QPVRFLAKRFAVKEAAAKAFGTGIRNGLAFNQFEVFNDELGKPRLRLWGEALKLAEKL
                     GVVNMHVTLADERHYACATVIIES"
     gene            25802..25882
                     /gene="shoB"
                     /locus_tag="PN977_001230"
     CDS             25802..25882
                     /gene="shoB"
                     /locus_tag="PN977_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin ShoB"
                     /protein_id="HDI6041285.1"
                     /translation="MTDCRYLIKRVIKIIIAVLQLLLLFL"
     gene            complement(26076..26336)
                     /gene="yfhL"
                     /locus_tag="PN977_001231"
     CDS             complement(26076..26336)
                     /gene="yfhL"
                     /locus_tag="PN977_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster ferredoxin YfhL"
                     /protein_id="HDI6041286.1"
                     /translation="MALLITKKCINCDMCEPECPNEAISMGDHIYEINSDKCTECVGH
                     YETPTCQKVCPIPNTIVKDPQHVETEEQLWDKFVLMHHADKI"
     gene            complement(26392..27240)
                     /locus_tag="PN977_001232"
     CDS             complement(26392..27240)
                     /locus_tag="PN977_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HDI6041287.1"
                     /translation="MNGLLRIRQRYQGLAQSDKKLADYLLLQPDTARHLSSQQLANEA
                     GVSQSSVVKFAQKLGYKGFPALKLALSEALASQPESPSVPIHNQIRGDDPLRLVGEKL
                     IKENTAAMYATLNVNSEEKLHECVTMLRSARRIILTGIGASGLVAQNFAWKLMKIGFN
                     AAAVRDMHALLATVQASSPDDLLLAISYTGVRRELNLAADEMLRVGGKVLAITGFTPN
                     ALQQRASHCLYTIAEEQATNSASISACHAQGMLTDLLFIALIQQDLELAPERIRHSEA
                     LVKKLV"
     gene            27449..28084
                     /gene="yfhb"
                     /locus_tag="PN977_001233"
     CDS             27449..28084
                     /gene="yfhb"
                     /locus_tag="PN977_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311453.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase C"
                     /protein_id="HDI6041288.1"
                     /translation="MATHERRVVFFDLDGTLHQQDMFGSFLRYLLRRQPLNALLVLPL
                     LPIIAIALLIKGRAARWPMSLLLWGCTFGHNEARLQALQADFVRWFRDNVTAFPLVQE
                     RLTTYLLSSDADIWLITGSPQPLVEAVYFDTPWLPRVNLIASQIQRGYGGWVLTMRCL
                     GHEKVAQLERKIGTPLRLYSGYSDSNQDNPLLYFCQHRWRVTPRGELQQLE"
     gene            28109..28645
                     /gene="tadA"
                     /locus_tag="PN977_001234"
     CDS             28109..28645
                     /gene="tadA"
                     /locus_tag="PN977_001234"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HDI6041289.1"
                     /translation="MRRAFITGVFFLSEVEFSHEYWMRHALTLAKRAWDEREVPVGAV
                     LVHNNRVIGEGWNRPIGRHDPTAHAEIMALRQGGLVMQNYRLIDATLYVTLEPCVMCA
                     GAMIHSRIGRVVFGARDAKTGAAGSLMDVLHHPGMNHRVEITEGILADECAALLSDFF
                     RMRRQEIKAQKKAQSSTD"
     gene            complement(28642..30198)
                     /gene="mltF"
                     /locus_tag="PN977_001235"
     CDS             complement(28642..30198)
                     /gene="mltF"
                     /locus_tag="PN977_001235"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltF"
                     /protein_id="HDI6041290.1"
                     /translation="MKKLKINYLFIGILALLLAVALWPSIPWFGKADNRIAAIQARGE
                     LRVSTINTPLTYNEINGKPFGLDYELAKQFADYLGVKLKVTVRQNISQLFDDLDNGNA
                     DLLAAGLVYNSERVKNYQPGPTYYSVSQQLVYKVGQYRPRTLGNLTAEQLTVAPGHVV
                     VNDLQTLKETKFPELSWKVDDKKGSAELMEDVIEGKLDYTIADSVAISLFQRVHPELA
                     VALDITDEQPVTWFSPLDGDNTLSAALLDFFNEMNEDGTLARIEEKYLGHGDDFDYVD
                     TRTFLRAVDAVLPQLKPLFEKYAEEIDWRLLAAIAYQESHWDAQATSPTGVRGMMMLT
                     KNTAQSLGITDRTDAEQSISGGVRYLQDMMSKVPESVPENERIWFALAAYNMGYAHML
                     DARALTAKTKGNPDSWADVKQRLPLLSQKPYYSKLTYGYARGHEAYAYVENIRKYQIS
                     LVGYLQEKEKQATEAAMQLAQDYPAVSPTELGKEKFPFLSFLSQSSSNYLTHSPSLLF
                     SRKGSEEKQN"
     gene            30458..34345
                     /gene="purL"
                     /locus_tag="PN977_001236"
                     /gene_synonym="purI"
     CDS             30458..34345
                     /gene="purL"
                     /locus_tag="PN977_001236"
                     /gene_synonym="purI"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HDI6041291.1"
                     /translation="MMEILRGSPALSAFRINKLLARFQAARLPVHTIYAEYVHFADLN
                     APLNDDEHAQLERLLKYGPALASHAPQGKLLLVTPRPGTISPWSSKATDIAHNCGLQQ
                     VNRLERGVAYYIEAGTLTNEQWQQVTAELHDRMMETVFFALDDAEQLFAHHQPTPVTS
                     IDLLGQGRQALIDANLRLGLALAEDEIDYLQDAFTKLGRNPNDIELYMFAQANSEHCR
                     HKIFNADWIIDGEQQPKSLFKMIKNTFETTPDHVLSAYKDNAAVMEGSEVGRYFADHE
                     TGRYDFHQEPAHILMKVETHNHPTAISPWPGAATGSGGEIRDEGATGRGAKPKAGLVG
                     FSVSNLRIPGFEQPWEEDFGKPERIVTALDIMTEGPLGGAAFNNEFGRPALNGYFRTY
                     EEKVNSHNGEELRGYHKPIMLAGGIGNIRADHVQKGEINVGAKLVVLGGPAMNIGLGG
                     GAASSMASGQSDADLDFASVQRDNPEMERRCQEVIDRCWQLGDANPILFIHDVGAGGL
                     SNAMPELVSDGGRGGKFELRDILSDEPGMSPLEIWCNESQERYVLAVAADQLPLFDEL
                     CKRERAPYAVIGEATEELHLSLHDRHFDNQPIDLPLDVLLGKTPKMTREVQTLKAKGD
                     ALVREGITIADAVKRVLHLPTVAEKTFLVTIGDRSVTGMVARDQMVGPWQVPVANCAV
                     TSASLDSYYGEAMAIGERAPVALLDFAASARLAVGEALTNIAATQIGDIKRIKLSANW
                     MAAAGHPGEDAGLYEAVKAVGEELCPALGLTIPVGKDSMSMKTRWQEGNEEREMTSPL
                     SLVISAFARVEDVRHTITPQLSTEDNALLLIDLGKGNNALGATALAQVYRQLGDKPAD
                     VRDVAQLKGFYDAIQALVAQRKLLAYHDRSDGGLLVTLAEMAFAGHCGIDADIASLGD
                     DRLAALFNEELGAVIQVRAADREAVESVLAQHGLADCVHYVGQAVSGDRFVITANGQT
                     VFSESRTTLRVWWAETTWQMQRLRDNPECADQEHQAKSNDADPGLNVKLSFDINEDVA
                     APYIATGARPKVAVLREQGVNSHVEMAAAFHRAGFDAIDVHMSDLLAGRTGLEDFHAL
                     VACGGFSYGDVLGAGEGWAKSILFNDRVRDEFATFFHRPQTLALGVCNGCQMMSNLRE
                     LIPGSELWPRFVRNTSDRFEARFSLVEVTQSPSLLLQEMVGSQMPIAVSHGEGRVEVR
                     DAAHLAALESKGLVALRYVDNFGKVTETYPANPNGSPNGITAVTTESGRVTIMMPHPE
                     RVFRTVSNSWHPENWGEDGPWMRIFRNARKQLG"
     gene            34922..36349
                     /gene="qseE"
                     /locus_tag="PN977_001237"
     CDS             34922..36349
                     /gene="qseE"
                     /locus_tag="PN977_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two component system sensor histidine kinase
                     QseE/GlrK"
                     /protein_id="HDI6041292.1"
                     /translation="MKRWPVFPRSLRQLVMLAFLLILLPLLVLAWQAWQSLNALSDQA
                     ALVNRTTLIDARRSEAMTNAALEMERSYRQYCVLDDPTLAKVYQSQRKRYSEMLDAHA
                     GVLPDDKLYQALRQDLNNLAQLQCNNSGPDAAAAARLEAFASANTEMVQATRTVVFSR
                     GQQLQREIAERGQYFGWQSLVLFLVSLVMVLLFTRMIIGPVKNIERMINRLGEGRSLG
                     NSVSFSGPSELRSVGQRILWLSERLSWLESQRHQFLRHLSHELKTPLASMREGTELLA
                     DQVVGPLTPEQKEVVSILDSSSRNLQKLIEQLLDYNRKQADSAVELENVELAPLVETV
                     VSAHSLPARAKMMHTDVDLKATACLAEPMLLMSVLDNLYSNAVHYGAESGNICLRSSL
                     HGARVYIDVINTGTPIPQEERAMIFEPFFQGSHQRKGAVKGSGLGLSIARDCIRRMQG
                     ELYLVDESGQDVCFRIELPSSKNTK"
     gene            36514..37227
                     /gene="qseG"
                     /locus_tag="PN977_001238"
     CDS             36514..37227
                     /gene="qseG"
                     /locus_tag="PN977_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system QseEF-associated
                     lipoprotein QseG"
                     /protein_id="HDI6041293.1"
                     /translation="MRHIFQRLLPRRLWLAGLPCLALLGCVQSHNKPAIDTPAEEKIP
                     VYQLADYLSTECSDIWALQGKSTETNPLYWLRAMDCADRLMPAQSRQQARQYDDGSWQ
                     NTFKQGILLADAKITPYERRQLVARIDALSTKIPAQVRPLYQLWRDGQALQLQLAEER
                     QRYSKLQQSSDSELDTLRQQHHVLQQQLELTTRKLENLTDIERQLSTRKPAGNFSPDT
                     PHESEKPAPSTDEVTPDEP"
     gene            37217..38551
                     /gene="glrR"
                     /locus_tag="PN977_001239"
     CDS             37217..38551
                     /gene="glrR"
                     /locus_tag="PN977_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator GlrR"
                     /protein_id="HDI6041294.1"
                     /translation="MSHKPAHLLLVDDDPGLLKLLGLRLTSEGYSVVTAESGAEGLRV
                     LNREKVDLVISDLRMDEMDGMQLFAEIQKVQPGMPVIILTAHGSIPDAVAATQQGVFS
                     FLTKPVDKDALYQAIDDALEQSAPATDERWREAIVTRSPLMLRLLEQARLVAQSDVSV
                     LINGQSGTGKEIFAQAIHNASPRNSKPFIAINCGALPEQLLESELFGHARGAFTGAVS
                     NREGLFQAAEGGTLFLDEIGDMPAPLQVKLLRVLQERKVRPLGSNRDIDIDVRIISAT
                     HRDLPKAMARGEFREDLYYRLNVVSLKIPALAERTEDIPLLANHLLRQAAERHKPFVR
                     AFSTDAMKRLMTASWPGNVRQLVNVIEQCVALTSSPVISDALVEQALEGENTALPTFV
                     EARNQFELNYLRKLLQITKGNVTHAARMAGRNRTEFYKLLSRHELDANDFKE"
     gene            38612..38950
                     /gene="glnB"
                     /locus_tag="PN977_001240"
     CDS             38612..38950
                     /gene="glnB"
                     /locus_tag="PN977_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017373484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulatory protein P-II"
                     /protein_id="HDI6041295.1"
                     /translation="MKKIDAIIKPFKLDDVREALAEVGITGMTVTEVKGFGRQKGHTE
                     LYRGAEYMVDFLPKVKIEIVVPDDIVDTCVDTIIRTAQTGKIGDGKIFVFDVARVIRI
                     RTGEEDDAAI"
     gene            complement(38995..40185)
                     /gene="hmpA"
                     /locus_tag="PN977_001241"
     CDS             complement(38995..40185)
                     /gene="hmpA"
                     /locus_tag="PN977_001241"
                     /EC_number="1.14.12.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NO-inducible flavohemoprotein"
                     /protein_id="HDI6041296.1"
                     /translation="MLDAQTIATVKATIPLLVETGPKLTAHFYDRMFTHNPELKEIFN
                     MSNQRNGDQREALFNAIAAYASNIENLPALLPAVEKIAQKHTSFQIKPEQYNIVGEHL
                     LATLDEMFSPGQEVLDAWGKAYGVLANVFINREAEIYNENASKAGGWEGTRDFRIVAK
                     TPRSALITSFELEPVDGGAVAEYRPGQYLGVWLKPEGFPHQEIRQYSLTRKPDGKGYR
                     IAVKREDGGQVSNWLHNHANVGDVVKLVAPAGDFFMAVADDTPVTLISAGVGQTPMLA
                     MLDTLAKAGHTAQVNWFHAAENGDVHAFADEVKELGQALPRFTAHTWYRQPSEADRAK
                     GQFDSEGLMDLSKLEGAFSDPTMQFYLCGPVGFMQFAAKQLVDLGVKQENIHYECFGP
                     HKVL"
     gene            40513..41766
                     /gene="glyA"
                     /locus_tag="PN977_001242"
     CDS             40513..41766
                     /gene="glyA"
                     /locus_tag="PN977_001242"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HDI6041297.1"
                     /translation="MLKREMNIADYDAELWQAMEQEKVRQEEHIELIASENYTSPRVM
                     QAQGSQLTNKYAEGYPGKRYYGGCEYVDIVEQLAIDRAKELFGADYANVQPHSGSQAN
                     FAVYTALLEPGDTVLGMNLAHGGHLTHGSPVNFSGKLYNIVPYGIDATGHIDYADLEK
                     QAKEHKPKMIIGGFSAYSGVVDWAKMREIADSIGAYLFVDMAHVAGLVAAGVYPNPVP
                     HAHVVTTTTHKTLAGPRGGLILAKGGSEELYKKLNSAVFPGGQGGPLMHVIAGKAVAL
                     KEAMEPEFKTYQQQVAKNAKAMVEVFLERGYKVVSGGTDNHLFLVDLVDKNLTGKEAD
                     AALGRANITVNKNSVPNDPKSPFVTSGIRVGTPAITRRGFKEAEAKELAGWMCDVLDS
                     INDEAVIERIKGKVLDICARYPVYA"
     gene            complement(41830..43023)
                     /locus_tag="PN977_001243"
     CDS             complement(41830..43023)
                     /locus_tag="PN977_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HDI6041298.1"
                     /translation="MRACINNQQIRHHNKCVILELLYRQKRANKSTLARLAQISIPAV
                     SNILQELESEKRVVNIDDESQTRGHSSGTWLIAPEGDWTLCLNVTPTSIECQVANACL
                     SPKGEFEYLQIDAPTPQALLSEIEKCWHRHRKLWPDRTINLALAIHGQVDPVTGVSQT
                     MPQAPWTTPVEVKYLLEEKLGIRVMVDNDCVMLALAEKWQNNSQERDFCVINVDYGIG
                     SSFVINEQIYRGSLYGSGQIGHTIVNPDGVVCDCGRYGCLETVASLSALKKQARVWLK
                     SQPVNTQLDPEKLTTAQLIAAWQSGEPWITSWVDRSANAIGLSLYNFLNILNINQIWL
                     YGRSCAFGENWLNTIIRQTGFNPFDRDEGPSVKATQIGFGQLSRAQQVLGIGYLYVEA
                     QLRQI"
     gene            43141..46422
                     /locus_tag="PN977_001244"
     CDS             43141..46422
                     /locus_tag="PN977_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5107 domain-containing protein"
                     /protein_id="HDI6041299.1"
                     /translation="MTPVKVWQERVEIPTYETGPQDIHPMFLENRVYQGSSGAVYPYG
                     VTDTLSEQKTLKSWQAVWLENDYIKVMILPELGGRVHRAWDKVKQRDFVYHNEVIKPA
                     LVGLLGPWISGGIEFNWPQHHRPTTFMPVDFTLEAHDDGAQTVWVGETEPMHGLQVMT
                     GFTLRPDRAALEIASRVYNGNATPRHFLWWANPAVKGGEGHQSVFPPDVTAVFDHGKR
                     AVSAFPIATGTYYKVDYSAGVDISRYKNVPVPTSYMAEKSQYDFVGAWCHDEDGGLLH
                     VANHHIAPGKKQWSWGHSEFGQAWDKSLTDNNGPYIELMTGIFADNQPDFTWLDAYEE
                     KRFEQYFLPYHSLGMVQNASRDAVIKLQRSERGIEWGLYAISPLNGYRLAIREIGKCN
                     ALLDDAVALTPATAIQGVLHGINPDRLTIELSDADGNIVLSYHEHQPQALPLPDVAKA
                     PLSAQDITSTDEAWFIGQHLEQYHHASRSPFDYYLRGVALDPLDYRCNLALAMLEYNR
                     ADFPQAVAYATQALKRAHALNKNPQCGQASLIRASAYERQGQYQQAEEDFWRAVWSGN
                     SKAGGYYGLARLAARNGHFDAGLDFCQQSLRACPTNQEVLCLHNLLLVLSGRQDNARL
                     QREKLLRDYPLNATLWWLNWFDGRSESALAQWRGLCQGRDVNALMTAGQLINWGMPTL
                     AAEMLNALDCQRTLPLYLQASLLPKAERGELVAKAIDVFPQFVRFPNTLEEVAALESI
                     EECWFARHLLACFYYNKRSYGKAIALWQRCVEMSPEFADGWRGLAIHAWNKQHDYELA
                     ARYLDNAYQLAPQDARLLFERDLLDKLSGATPEKRLARLENNLEIALKRDDMTAELLN
                     LWHLTGQADKAADILATRKFHPWEGGEGKITSQFILNQLLRAWQHLDAREPQQASELL
                     HAALHYPENLSEGRLPGQTDNDIWFWQAICANAQGDETEAMRCLRLAATGDRTINIHS
                     YYNDQPVDYLFWQGMALRLLGEQQIAQQLFSEMKQWAQEMAKTSIEADFFAVSQPDLL
                     SLYGDLQQQHKEKCLMVAMLASAGLGEVAHYESARAELTAINPAWPKAALFTTVMPFI
                     FNYVH"
     gene            46519..47502
                     /locus_tag="PN977_001245"
     CDS             46519..47502
                     /locus_tag="PN977_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HDI6041300.1"
                     /translation="MPKKMRTTRNLLLMATLLGSALFARAADKEMTIGAIYLDTQGYY
                     AGVRQGVQDAAKDSSVQVQLIETNAQGDISKESTFVDTLVARNVDAIILSAVSENGSS
                     RTVRRASEAGIPVICYNTCINQKGVDKYVSAYLVGDPLEFGKKLGNAAADYFIANKID
                     QPKIAVINCEAFEVCVQRRKGFEEVLKARVPGAQIVANQEGTVLDKAISVGEKLIISR
                     PDLNAIMGESGGATLGAVKVVRNQNQAGKIAVFGSDMITEIAQELENNQVLKAVVDIS
                     GKKMGNAVFAQTLKVINKQADGEKVIQVPIDLYTKTEDGKQWLATHVDGLP"
BASE COUNT        11470 a        11984 c        12633 g        11448 t
ORIGIN      
        1 tagcgcggtg gtcccacctg accccatgcc gaactcagaa gtgaaacgcc gtagcgccga
       61 tggtagtgtg gggtctcccc atgcgagagt agggaactgc caggcatcaa attaagcaga
      121 aggccatccg tcaggatggc ctttttgcat tggtgcagaa aaaaatgcct gatgcgatgc
      181 tgcgcgtctt atactcccac atatgccaga ttcagcaacg gatacggttt ccccaacttg
      241 cctacttcca tacgtgtcct ccttaccaga aatttatcct taagctcctc aataaccatt
      301 ttcctgctaa ctaaattcat ggttaaggtt gcataatgat acgcaacaaa tgtataacat
      361 ttttttacca aaaaaaataa acaacagcga ctgacaaaag catcggatta cggcaggaga
      421 cataatggca tggctgaaag tactgtaacg gcagacagca aactgacaag tagtgatact
      481 cgtcgccgca tttgggcgat tgtgggggcc tcttcaggta atctggtcga gtggttcgat
      541 ttctatgtct actcgttctg ttcactctac tttgcccaca tcttcttccc ttccgggaac
      601 acgacgactc aactactaca aacagcaggt gtttttgctg cgggatttct gatgcgccca
      661 ataggcggtt ggctatttgg ccgcatagcc gataaacatg gtcgcaaaaa atcgatgcta
      721 ttatcggtgt gtatgatgtg tttcggctca ctggttatcg cctgtttgcc aggctacgaa
      781 acaatcggta cctgggctcc ggcattattg cttcttgctc gtttattcca gggattatcc
      841 gttggcggag aatatggcac cagcgccacc tatatgagtg aagttgccgt tgaagggcgc
      901 aaaggttttt acgcatcatt tcagtatgtg acgttgatcg gcgggcaact gctagctcta
      961 ctggttgtcg tggttttaca acacaccatg gaagacgctg cactcagaga gtggggatgg
     1021 cgtattcctt tcgcgttagg agctgtgtta gctgttgtgg cgttgtggtt acgtcgtcag
     1081 ttagatgaaa cttcacaaca agaaacgcgc gctttaaaag aagctggatc tctgaaagga
     1141 ttatggcgca atcgccgtgc attcatcatg gttctcggtt ttaccgctgc gggctccctt
     1201 tgtttctata ccttcaccac ttatatgcag aagtatctgg taaatactgc gggaatgcat
     1261 gccaacgtgg cgagtggcat tatgactgcc gcattgtttg tattcatgct tattcaacca
     1321 ctcattggcg cgctgtcgga taagattggt cgccgtacct caatgttatg tttcggttcg
     1381 ctggcagcca tttttaccgt tcctattctc tcagcattgc agaacgtttc ctcgccttat
     1441 gccgcttttg gtctggtgat gtgcgccctg ctgatagtga gtttttatac ttcaatcagt
     1501 ggaatactga aggctgagat gttcccggca caggttcgcg cattaggcgt tggtctgtca
     1561 tatgcggtcg ccaatgctat atttggtggg tcggcggagt acgtagcgtt gtcgctgaaa
     1621 tcaataggaa tggaaacagc cttcttctgg tatgtgacct tgatggccgt ggtggcgttt
     1681 ctggtttcac taacattgca ccgtaaagga aaagggatgc ggctttagtg acgagtcagt
     1741 tgccagacgg tatagccggt gcttgcaccg gcgatatccc aggcaaaatc cttccagctc
     1801 cagccgctcc cctcagggcg gctatcccaa agctcttttg acgcccccag actgacagag
     1861 aacatcaatc caaacatggc actgcgatcc cggctcatcc cctgatgctg tgcatattca
     1921 tttccggcgg cggaaagcat cgccgaggcg ataaagtgtt gagctttatc ctgcccgctc
     1981 cagctatcgt tagccatatg gctgcatcca gaaagtaaca atagcgagca gacaaaaaga
     2041 atacgcaaaa cgtggcctcc aaaaaacaaa ccccgtacag gacggggttg tgattacagg
     2101 atgcggctaa ttaatcggtc gatgcggata cggcgcaaac gacggatgag tttacgaacc
     2161 ttcaccggat aatcggcaat actttgcaga tcgcgatagt gcttaacgat ggtggtatgc
     2221 tcgcggatca gctccagttc tttctctcgc tgtggcgcaa gctccagttg cggatcgtgg
     2281 atcaaaatgg cgttttccag atccagacgc caggcgcgcg ggttcaggtt attaccggtg
     2341 atcaacatcc acttatcatc aacccacatc cctttcaggt gataggtgtt gtcgtcatct
     2401 ttccataacc gaaccactaa ttggtcagta ttgacgtaat actgcaaacg gctcaggaaa
     2461 cgacgcagat tgatctcata gagataaggc aatgcgccaa ttatcttgaa aggttcatct
     2521 tccggaatgt agaagtcatt cgcggtttta tcaccaacaa taatttcgac ctttttccct
     2581 tcgcgcagca actggataat attgcgcaca aggattgctg gcaggttgaa gtaaggtgtg
     2641 cagatggtta gtttctgctc ggcacaaggc ataagatgga aaatagtctt gttcaacaga
     2701 ctcgatttcc ccagccccac tagcggcgtt acggaaagct gatcgttgtc ggcatcgccc
     2761 tggaaatgat aagcggcatc acgcagctcc tggcggaaca ggcgaatgtc gttcttgatt
     2821 tccgggcttt ttggccgatt aacatcatcc agacgattaa cgccgcggcc attcataata
     2881 ttctgtgtaa cccattcaaa cataatgtct gacatcttac ggttacggat cagatgataa
     2941 cggtcgtagc gatatttatc gtgctgatgc aggtaaacat cgttcaggct ggcaccgcta
     3001 taaagtacgc tatcgtcgat gataaagcct ttaaagtgca gaacaccaag ggcttcacgg
     3061 gtattgattg gaacgccata aaccggaaca tctacgcccg gattttcctg cgccatgcgg
     3121 cagtaccagt cagcgttagt gttagatgcc gcagcgccaa tgcgtccacg ttgtgcacga
     3181 tgccagtcga ccagcacccg cacatccagt tccggacgct gccttttagc ctcatacaac
     3241 gcgttcagaa tgcctttgcc accgtcatcc tgttcgagat acagggcgac aatgcaaatg
     3301 cgctgcttcg cgctggctat tttttccagc agcgtctccc ggaagtcggc gggagcgtaa
     3361 aagaaatcga catcatcaac tgattgagaa atcttgggta gttgggcaag gtgttgttga
     3421 tgtttattac gcttaaattt tgacaacatc acagtgcatt tcttctctgt tcattgaagg
     3481 gtcctctgtg caatgcagac gacataagcg ggcaataata acaccagtcc cgattaagtg
     3541 gtcaacattt ccagtacctt actcatgatt cctcgcgctg ggcaagattt agcgtaagcc
     3601 caacgatccc ctcttcgagc tggatatcaa cgttaaaccc gagcttgcgg gccagcgcca
     3661 ccatgccacg attgtttggc atcgtaatac cattcagacg ttgcagtccg tgatctcgcg
     3721 tataggtaat caacttttcc attaagcgtc gacctaagcc taaccctttg agatccgagc
     3781 gaaccagcac ggcaaattcg gcatcgatgt tatcagggtc ggagatcgca cgcgtgacgc
     3841 cgaggatctc ttccgtttga tcaatacgtc gtaccgctac aaatgccatt tcccgatcgt
     3901 agtcgatctg cgtcatgttg gctaaatctt catgggtaaa ttcgttgatc tcgctaaagt
     3961 agcggtaata aagatcttct ttggtgactc gcgaaatgaa ctgctgaagt tgtggctcat
     4021 cttctggcaa aatcgggcgg aacaagcagc gttcaccgtt tttcaattct acccactctt
     4081 ccagctgatg cggataaggg cgcactgcca gccggctctc gttatcgcct tcaaacggcg
     4141 cgatatccag cgtgacatcc agcgcggtaa attcactgcc agaagccagc aaaggatgaa
     4201 tatccagacg ttgaatttcc ggacaatcga caatcaagtt ggaaacctgt accagaagct
     4261 ggctcaagcc tgcaacatcc aatgggcgta gcgcactgcg cgcacgaatc tttttacttt
     4321 tgatcccctg aataaccaga tagcgggcca ggttcatgtt cagcggtggt aacgcgacga
     4381 cggcttgatc ttcaggacgc cactccacac cgccttcgcc cagcatgatc aacggcccga
     4441 aaaccggatc gtgctcaacc acaacccgca actcctgagc gccagcacgg ttagccatac
     4501 tttgcaccaa caggccgtgg atccgcgcct gcggccaggc catttttacg cgatcgaaaa
     4561 tagcgtttgc cgcttgctgg acttcattag ctgtacgcag gtagagcatg acgccctgaa
     4621 cttccgattt atgtggaata tccggcgaac gcaatttcag cgccaccgga taaccaatct
     4681 gttcggcaat atgcaccgct tcggtgctat cgctggcaat ccaggtgggg agcgtgttca
     4741 tgccatatgc ttgcaggatg ggctgaactt catgggtatc gagtgacgtt gctccttcgg
     4801 caatcgcctg ttgcaacaga agatgcgctt ctgcggtatt ggaggtcaga ttgctgggca
     4861 acgccggcgt ttcgcgtagt tgcttctgat tacgccggta ctccaccata tgcataaaag
     4921 cagtgatggt tccttccggc gtgcgatagg tcggcagccc ggcttcactg aataatcgtc
     4981 gtgcctcttg cgaggagtgc tcgccacacc agttcgtcag cagagaaaca tacttgctgc
     5041 ggggatgatg ctttaccgct tcaattaatg cttgcgcgct ttctgttgcg ggagcagcgg
     5101 cgctgggcga atgaataacc atcagcgcgt caaaatcctg gctgtggagc aggatatcca
     5161 gcgttttaat atagtgctca ctgctggcgt catcgcgtag atcgagcggg ttagagattg
     5221 ccacatgttc tggcagtgca tcgcgcagtt tctggcaggt ttcttcgctt agcgttgcca
     5281 gcttgccatt gcgtgaccat aaggcatcca gcgccagcgc ggcaggcgca gcaccgttgc
     5341 tgataatcat caaccggtca ccacgcagcg ggcgcatatg gctaagggtt tccaccgccg
     5401 aaaacagttc gtgggtatcc tgtacccgca acaaaccggc acgctgaata gccgcatccc
     5461 acgctggatc cattcctgcc gtggtgttca acagacgttg agccgccggg ctacgtccgc
     5521 ttttaatcac cagaatcggt ttattgcgcg aggcactacg ggccgccgaa acaaagcgtc
     5581 gcgcgtcgct taactgttcg agatagagca ggatggcgct ggttttactg tcgcgcgcca
     5641 gatagtcgag caattcatca acgtcgatat ccaggctgtc gccgagcgca ataaagtagg
     5701 aaaagcccat ctcacgctgt tgcgcccagt cgaggatggt gttggagaca gcagccgatt
     5761 gcgaaataaa cgccagcttg ccgcgtttaa tcggcacagg cgaaaagctg gcattcagac
     5821 cttgccaggg agccagtaaa cccagactgt ttggtccaag caggcgcatg ttatggcgca
     5881 gggcgcaggc gcggagatct tcgtgttgcg atgacggggc ggaaagaata atgcaggttt
     5941 tacagccttt ctcgccgagc tcttccagaa gagcaagatt acggctggca ttggtacata
     6001 aaaccgcaag gtctggtgta aagggcaagc tggcaatatc cggccaggcc aacacaccca
     6061 acactgcttt ccaggcaggc gtcaccggga gtaccggtcc gttaaagcct cccgccagca
     6121 ggttacgcat catcaggtaa cctgcgcgat tgggtttcat cgatgcgcca attaccgcaa
     6181 tcgattttgg tcgcagtagt gcttccagtc ctcgctgact cataccggtc tcccgcagaa
     6241 gtgaccgaat gattttaaac gctttctaac tgttctgctg tgatgctacc cagatgttgc
     6301 gtttttcctg ccagatagcg tgttttaaag cgggtaaaat gctcgcctaa ccctgccgcc
     6361 gccccggtat cgccgaccat atctaacagt gcgatggcta cctcggcagt acaatattgg
     6421 ccttcagcct gggcttcacg caggcgatag gcagaaagcc gggaaagatc gacggaaatg
     6481 acgggaagat tatccagata cggactttta cgaaacatct tgcgagcttc cggccaggta
     6541 ccatcgagca tgataaatag cggaggctta ccggcaggtg gtgtgaagat cacttcccgt
     6601 tgctcatcag catacgaggc gggaaagacc accattggct gatagtccgg gttttgtacc
     6661 agatccagca aatctagcga gggttcggta cgcgaccatt gaaacgcaac ggtatcaggc
     6721 aaaatatcag caatcagacg cccggtatta ctgggcttca ttggctcggt gtcgaacatc
     6781 agcaaacaga agcgactttt tgcttgtgct ggggtaattg tcgaacagag acataatttc
     6841 tctggcaaaa gacagcgttg gcagcgacga acgcgattac cgcgggcaag aaaaggacgt
     6901 gttgcgcgcg caatacgctc ggcgcgtaac tggagaacag cgttttcggt cataagagag
     6961 cgtcgaaaaa acgccattgt cgcagaggag aaaacggggc acaagatgcg ccccggtaag
     7021 attaaagaga ttcgttcagc cagctatcaa acggcgcttt cggtactgcg ccattaagca
     7081 tgtcgacaac ctgaccgttt ttgaaaatca tgatcgtcgg tatactacga attccaaagc
     7141 gactgcttaa ttcacgttca gcttcggtat tcactttcac aaagcgcact ttaccgctac
     7201 gctcttgcgc gacatcttca aaaattggtg cgaaattacg gcaggggccg caccacggtg
     7261 cccagaagtc gatcaccaca ggtagatcat ctttcagcaa tttgtcgagc gtttcaccgg
     7321 tcgcattaat cacctctccg tcaaacaagt cgtgaccgca gcgtccgcat tttgccgcat
     7381 cttcgatccg atcgtcggga atgcgattga tggcctgaca atgggtacaa acggtattca
     7441 taactaacct cgggatgagt aaggagactt atcggcaacg acgccggtat gtttctaata
     7501 tgttacatat tatcggggac attgttccag acaacaatct gttttattca atgggtatga
     7561 tagacacagg ctttggaacg aaatcatggc gaaccgcgcg gacttcgggt aatctgcgcg
     7621 cttcgcgcag cgctggtgga gaaaagcatg aacgatgaaa tgaaaggtaa aagcggcaag
     7681 gtcaaagtga tgtatgtccg cagtgatgat gattctgata aacgtaccca caacccgcgt
     7741 accgggaaag ggggcgggcg tccaggaaaa tctcgtgctg acggtggccg tcgccccgcc
     7801 cgcgatgaca aacagagtca gccccgtgac cgcaagtggg aagattcgcc gtggcgcacg
     7861 gtttcccgcg cgccgggtga tgagacgccg gaaaaggccg atcacggtgg catcagtggt
     7921 aaaagtttta ttgatccgga agttttgcgt cgtcagcgtg cggaagaaac ccgcgtctac
     7981 ggagaaaacg cctgtcaggc actgttccag agccgtccgg aagcgattgt tcgcgcctgg
     8041 tttatccaga gcgtaacgcc acgttttaaa gaagccttgc gctggatggc ggcaaaccgc
     8101 aaagcgtacc atgtggtgga tgaagcggaa ctgacaaaag cgtcaggcac ggaacatcac
     8161 ggcggcgttt gcttcctgat caaaaagcgt aacggtacaa ccgtgcagca gtgggtaagc
     8221 caggctggcg cgcaggattg tgtactggcc ctggaaaacg aatctaaccc gcataacctg
     8281 ggcggcatga tgcgtagctg tgcgcacttc ggcgtgaaag gtgttgtggt gcaggatgca
     8341 gcactgctgg agtctggggc ggctatccgt accgcagaag gcggcgcaga gcacgttcag
     8401 ccgattactg gcgacaacat tgttaacgtg ctggatgatt tccgtcaggc gggttacacc
     8461 gtagtgacaa cttccagcga gcagggtaaa ccgctgttca aaaccagtct gccagcgaaa
     8521 atggtactgg tgctgggtca ggaatatgaa gggttaccgg atgccgcacg cgatccgaac
     8581 gatctgcgcg tgaagattga tggtactggc aacgttgccg ggctgaatat ctctgtcgca
     8641 accggcgttc ttcttggcga atggtggcgt cagaataaag cctgataaat cagccgggtg
     8701 gcaactctgc catccggcat tttcccgcaa atttactcac tctctgccgg taatactggc
     8761 atccagtcaa tcggcgtctc gccacgttgt tccagccact gatttgccag cacaaaatgg
     8821 ttgcagccaa agaatccacg atgcgccgaa agcggcgacg gatgcggtgc tttcagtaca
     8881 tgatggcgtt gcttatctat aatcgcccct ttcttttgcg catgcgatcc ccacaacaaa
     8941 aacaccacgc cttcgcgatg ctggttaatc aggctgatca ctttatcggt gaaggtttcc
     9001 cagccgaggc tggcgtggga atgcgcctga cctgcgcgta ccgtcaacac agtattgagt
     9061 agcagaacgc cctgacgcgc ccagctttca agataaccat gattagggcg ggtgaaaccg
     9121 ggaatagtat tttccagctc tttatacata ttcagcagtg acggcggtgt cgcaatgccg
     9181 gggcgaacgg aaaatgccag accatgcgcc tgtcccggtc cgtgataagg atcctggccg
     9241 agaatcacca ctttaacgtc acccaactct gtaaagcgga acgcgttaaa gacatccttt
     9301 tgtggcggat agatagtgac gccggactgc cgctcgctgg cgacggtctg aagggtatta
     9361 agaaaatagg gttgctgctt ctcttcagcc agcacatcat gccaggttaa ttcgttagcc
     9421 atctcgctct cctgcgaatc ttcaatccgc ctagcttaac tgcttctttc tacagaacaa
     9481 aatcatgctc attttttaga cttaaacgag tgcaaaaaaa tgtaaattgg taaaatttca
     9541 cacctgcata agttgatgta aaacaataaa atccatttgt gaccacattc gatatgtggt
     9601 ttctattgat ttaaatcaaa gaatcaaggg tgtttgagga gtatatatac actcaagcaa
     9661 caatggtttt accaattggc cgcgacaggc tgaacaaatc aaataatttt gccggggagg
     9721 catcacatga ttacaggtat ccagattact aaagccgcta acgacgatct gctgaactct
     9781 ttctggctgc tggacagcga aaaaggcgaa gcgcgttgca tcgttgcgaa agcaggtttt
     9841 gcagaagacg aagtggttgc agtaagcaaa ctgggtgaca ttgagtaccg tgaagttcca
     9901 gtagaagtga aaccagaagt tcgcgttgaa ggtggtcaac acctgaacgt aaacgttctg
     9961 cgtcgtgaaa ctctggaaga tgcagttaag catccggaaa aatatccgca gctgaccatc
    10021 cgtgtatccg gttatgcagt tcgctttaac tctctgactc cggaacagca gcgcgacgtt
    10081 atcgctcgta cctttactga aagcctgtaa ggcttaagta gcaagttact aagcggaaga
    10141 gggcgacctc ttccgctttt ttcattaata gaaaatgcgt accgcgcaat agaccagcag
    10201 cagcgcgagc acgatattta actggcgacc atactggcga aacaatcgct gaaacaggtg
    10261 ccccgccagc gcccagcaca cattgccaaa cgtcccaatc atcgccagca aaacgctgac
    10321 gccaatcacc cagcttaacg cctgcgtttg tggcaggaca aatgtcgaca gtgctgtaat
    10381 gccatacaaa atgattttga cgttcacaaa ctgcaaagca aagctggccc aaaaactgat
    10441 tggttttgtc tgaagcccat cttcctttgt tggactggta gcgattttcc acgccagcca
    10501 gacaatatat gccgcccccg cccaactcaa aaggtgtacc actgccgggt caatcactgc
    10561 cagtgaaaat gaaatgcccg cacacagtaa catcacaatc aaaaatccca gactcatccc
    10621 tgccagcacg cgagtacttt gacgaaatcc atgcgacgta gcagagctaa gggcgagaat
    10681 attgttcggt cctggcgtca tggcggtaat cagggtgtaa gtccaaaaag cacttaaaag
    10741 ggtcggtgtc acttgttatc tcctctgttt tggtagacag tacccgacac gaaacattta
    10801 aaaaaatgaa tgttttaata ttctaaattc gatattttcg ataggtttgg ggttatggat
    10861 ctgcgccgtt ttattacact taaaaccgtg gtggaagagg gttccttttt gcgagcttcg
    10921 caaaaattgt gctgcacaca atcgacggtg acttttcata ttcagcagct tgagcaggag
    10981 ttctcagtcc agttatttga gaaaattggt cgacgaatgt gccttacccg cgaaggaaaa
    11041 aagctgctgc cgcacatcta tgaactcacc agagtgatgg atacccttcg tgaggctgcc
    11101 aaaaaagagt cggatccgga cggggaacta cgcgttgttt caggcgaaac gttgctttct
    11161 taccgaatgc cgcaggtgtt gcagcgtttt cggcaacgcg caccaaaagt gtgtttatcg
    11221 ttgcagtctc tgaactgtta cgtgatccgt gatgcgctac tgaatgatga ggctgatgtc
    11281 ggcgtctttt accgcgtagg gaatgatgat gctctgaacc gacgagagtt gggtgaacaa
    11341 tcgctggtgc tggtagcttc accgcaaatt gcagatgtcg attttactga gccgggaaga
    11401 cataacgcct gtagttttat tatcaatgaa ccgcaatgtg tcttccgcca gatatttgag
    11461 agcacgctgc gccagcgacg gatcacggtg gaaaacacca ttgagcttat aagtatcgaa
    11521 agcatcaagc gttgtgtcgc ggcaaatatc ggggtcagtt acctgccgcg ctttacagtg
    11581 gcaaatgaac ttgcgagtgg cgaattaatc gaattgcctt ttggcgaaca gtcgcagacc
    11641 attacggcaa tgtgcgctca ccatgccgga aaagcggtta gcccggcgat gcacattttt
    11701 attcagtgtg ttgaagagtg ttttttgccg ggataaaaat aatgccgggc ttaaacccgg
    11761 cattgagatt actcttctgt cgtttgcggt ggcacgcccg ttccgctcgg tttacggcgc
    11821 ttaccaatat ttttggtgtc gcgatggcgt tttttcaccc gcggcttctc tttctcttta
    11881 gcttttttct tctcggcgcg ttttgccagt actttcttcg atggcttgcc ggtctgcttc
    11941 tcgcttggcg cacgcgtttt cgggcgtaac tcgtcaatta cgcgagcttt aattggctct
    12001 tcaatatagc gaccaacttt gcccagcagc agatggtcat gggcttccac cagcgaaatc
    12061 gcggtgcctt tacgaccggc gcgcgcggta cgtccgatac ggtgcaaata agtatcgcca
    12121 ctgcgcggca tatcgaagtt aaagacgtgg ctgacgtcag gaatgtcgat accgcgcgcg
    12181 gcaacatcgg ttgcaaccag tacgtttacg cgaccttcgg tcaaacgctt gatcgcttcg
    12241 ttacgcttgc cctgtaccat ctcaccttcg agatagcagt tgttgatgcc cgcttcgcgc
    12301 agccagtttg ccagctcatg cacacgctca cgcttacgca caaacacaat tgagcgggtc
    12361 gcttccggct gttttaacag gtgcaccagc aatgcggttt tatgttccag gtcatcggcg
    12421 cggtaatacc actgatgaat ttttttgcgc tcacgggtgg acggattggc agaaacctcc
    12481 accggatctt ccagcagacg ctcggcaaag tcctgaatcg catcgccttc cagcgttgcc
    12541 gaaaagagca gggtctgttt acgccagcgc gtttcgccag caatatgttc gatatcctga
    12601 gcgaagccca tatccagcat acggtctgct tcgtcgagga tcagcgtttc aaccgcgcgg
    12661 caatcgaagt tctcttcttt tatgtattgc agcagacgtc cggtcgtggc gaccacgatg
    12721 tcctgatttt cgctgaacac ttccgcgtgg ttcatatagg ccacgccgcc ggtgatggtg
    12781 gcgatatcca gatgcgtatg tttcgccagt tcgcgggcat gatcggccac ctgcatcgcc
    12841 agctcgcgag ttggggtgag gatcaaaata cgcggcggac cggatttctt acgtgggaaa
    12901 tcgagcaggt gctgcaacgc tggcagcaga tacgccgccg ttttaccggt gcctgtcggc
    12961 gcagaaccga gtacatcacg gccatcgagc gcaggcggaa tggcggcagc ctgaatggcg
    13021 gtcgggcgag tgaaaccttt atcctggagg gcttccagca ggctttcgtc gagttcaagt
    13081 tcggaaaaag tcgttacagt catgttctac ctctgtgtgg ggcgctgatt atagacgtta
    13141 cggctgcaat cttcatctgt ttgtatggat atcgcttctc gggtattgtt ctcacccggt
    13201 gctatcgccg tttttcctgc aaggttttat tttcatgtca cagtctacat ccgtgcttcg
    13261 tcgtaatgga tttactttta aacagttttt tgttgctcac gatcgttgtg cgatgaaagt
    13321 gggaacggat ggcattttgc tgggcgcatg ggcaccggtg gctggggtaa aacgttgcct
    13381 tgatatcggc gcgggtagcg ggttgctggc attaatgctg gcgcagcgaa ccgatgacag
    13441 cgtaatgatt gatgcagttg aactggaaag tgaagctgcc gcgcaggcgc aggaaaatat
    13501 caaccagtcc ccgtgggcag agcggattaa cgtccatacg gcggatattc agcagtggat
    13561 cacacagcag acagtacgct tcgatttaat catcagtaac ccaccttact atcagcaggg
    13621 agtagagtgc tcgacacctc aacgggaaca ggctcgctat accactacgc ttgatcaccc
    13681 atcgttgctg acctgtgcgg cggagtgcat taccgaagag ggatttttct gcgtggtgct
    13741 gccagagcag attggtaatg gttttactga actggcatta agtatgggct ggcatttacg
    13801 tttgcggacg gatgtggcgg aaaacgaagc gcgactgccg catcgggtgc tgctggcatt
    13861 ctccccgcag gcgggagaat gctttagcga tcgcttagtc attcgtgggc cagaccagaa
    13921 ctattccgaa gcgtatacgg cgctgaccca ggctttttat ctgtttatgt aatgattgcc
    13981 gggagaaagg atcgacggac cggaatgggt gagcagttcc ggataatcca gcgtgaaatg
    14041 caacccccga ctctccttgc gcatcatcgc acagcgaaca atcaactcgg caacctgtac
    14101 cagattacgc agctccagca aattatttga gacgcggaaa tgggcgtaat attcgtctat
    14161 ttcttgttgg agcatggtta tccgccgcag ggcgcgttcc aggcgcttcg ttgtgcgcac
    14221 aatgccaacg taatcccaca taaacagacg tagctcgtgc cagttatgtt gaatcaccac
    14281 ccgttcgtca gggttctcaa cacggctttc atcccacggc ggtaacgtac tgacgtcgtg
    14341 ggcataaggc atacgcctgg taatatcctc cgccgctgac cagccgtaaa ccagacactc
    14401 cagtaatgaa ttcgaggcca tgcggttagc gccgtgtaag ccggtataac tcacttcgcc
    14461 aatggcgtac aaaccttcga cgtccgtacg cccatgatca tccaccatta caccaccgca
    14521 ggtatagtgt gcagcaggca caatcggcac agggcctttc gtgagatcaa tccccagtcc
    14581 caataacttt tcataaatca tcgggaaatg ctgacgaata aaatcggcgg gcttatggct
    14641 gatatcgagg aacatacaat ctgcgccgag gcgtttcatt tcgtgatcaa tagcacgggc
    14701 gacaatatcg cgcggagcca gttcgccgcg ctcatcaaaa tcgggcataa aacgcgtgcc
    14761 atccgggcgc ttgagataag cgccttcgcc gcgcagtgct tctgttaaca ggaaattgcg
    14821 tgcctgtggg tgatacagcg cggtagggtg gaactgatta aattcgagat tggcaacccg
    14881 gcagcctgcg cgccacgcca tagcaatgcc atcgccagaa gaaatatccg gattggtggt
    14941 gtactgataa accttcgacg caccgccggt tgccagtacc actgcttttg cgtggcaggt
    15001 ttccaccgtt tctttatttc ggttccatac ccacgcgcca acaacccgtc gcgtgcccgg
    15061 caggccaatt ttgtcagaaa caatcagatc aaccgcgttg ctgcgctcca gcacgcgaat
    15121 attcggatgg ttcagcgcct tgctcaccag cgtggtttct acttctctac cggtggcgtc
    15181 ggcggcatga agaattcgac ggtgactatg tccaccttca cgggtcagat ggtagctttc
    15241 tgcgccattc ggttgaatgt gggtatcaaa caacaccccc tggtcgatta gccattgcac
    15301 acaggatcgt gcattgctgg cgacaaattc aactgcatga cgatcgcaaa taccagcccc
    15361 ggcaatcaat gtgtcttcca catgcgagtc aatgctgtca gtctcatcaa acacggcggc
    15421 tataccgccc tgggcataaa atgttgaacc ttccgttacc gggcctttac ttagaacgat
    15481 gacctgatgc tggtcagcca ggcgtagcgc cagtgaaagt ccagctgcgc cgctaccgat
    15541 aatcaacacg tcacatgaat gttcagggag agtattcatt ttctttgttt aatttactaa
    15601 acatggtttg gtcagcatag catcatgttg tgcgaataaa cacctgctat tttaatattt
    15661 gttacagttg ctaaacacgc tgacgcaggg cggcgagaaa aagagaagtt actggctggt
    15721 ggaggattag gtggtgaaat aaaaaggccg ttgggttact cttcaggcag ttaaatgggc
    15781 atttctacac agataatgcg atgttcagat tctgtagact tataatgata gataatgatc
    15841 cgtctacagc atgacaaaca aaaacagatg cgttacggaa ctttacaaaa acgagacact
    15901 ctaacccttt gcttgctcaa attgcagcta atggagtggc gtttcgatag cgcgtggaaa
    15961 tttggtttgg ggagacttta cctcggatga gcgagcagtt aacggaccag gtcctggttg
    16021 aacgggtcca gaagggagat cagaaagcct ttaacttact ggtagtgcgc tatcagcata
    16081 aagtggcgag tctggtttcc cgctatgtgc cgtcgggtga tgttcccgat gtggtacaag
    16141 aagcttttat taaagcctat cgtgcgctgg attcgttccg gggagatagc gctttttata
    16201 catggctgta tcggattgct gtaaatacag cgaaaaatta cctggttgct caggggcgtc
    16261 gtccaccttc cagtgatgtg gatgccattg aagctgaaaa cttcgaaagt ggcggcgcgt
    16321 tgaaagaaat ttcgaaccct gagaacttaa tgttgtcaga agaactgaga cagatagttt
    16381 tccgaactat tgagtccctc ccggaagatt tacgcatggc aataaccttg cgggagctgg
    16441 atggcctgag ctatgaagag atagccgcta tcatggattg tccggtaggt acggtgcgtt
    16501 cacgtatctt ccgagcgagg gaagctattg ataacaaagt tcaaccgctt atcaggcgtt
    16561 gacgatagcg ggatactgga taagggtatt aggcatgcag aaagaacaac tttccgcttt
    16621 aatggatggc gaaacgctgg atagtgagct gcttaacgaa ctggctcata acccagaaat
    16681 gcagaaaacc tgggaaagct atcacttaat ccgtgactca atgcggggtg atactcccga
    16741 ggtgctccat ttcgatatct cttcacgagt gatggccgcc attgaagaag agccagtacg
    16801 tcaaccggcg acattgatcc cggaagccca gcctgcgccg catcaatggc agaaaatgcc
    16861 attctggcag aaagtacgtc cgtgggcggc acagcttacc caaatgggcg tagccgcatg
    16921 cgtatcgctt gcagttatcg ttggcgtcca gcactataat ggacaatctg aaacgtccca
    16981 gcagcccgaa acgccggtat ttaatacact gccgatgatg ggtaaagcca gcccggtaag
    17041 cctgggagta ccttctgaag cgaccgcaaa caatggtcaa cagcagcagg tacaggagca
    17101 gcgtcgtcgc attaatgcaa tgttgcagga ttacgaactg caacgccgac tccactctga
    17161 acagcttcag tttgagcagg cgcaaaccca gcaagccgct gtacaggtgc caggaattca
    17221 aactttagga acgcaatcgc agtaatgaag caactttggt ttgccatgtc attagtgaca
    17281 ggtagcctgt tattctctgc taacgcctcg gccactcccg cgtccggggc gttattacag
    17341 cagatgaacc tggccagtca gtcactgaat tacgagctgt cattcatcag catcaataaa
    17401 cagggtgttg agtctctgcg ttatcgacat gcacgcctcg ataaccgtcc tcttgcacaa
    17461 ttgttgcaaa tggatggccc gcgccgggaa gtggtacagc gcggcaatga gatcagctat
    17521 tttgaaccgg gacttgaacc gttcacgctt aatggcgatt acattgttga ttctctgcca
    17581 tcgctcatct ataccgattt caaacgcctt tctccttact acgactttat ctccgtcggg
    17641 cgcacgcgta ttgctgatcg tctttgcgaa gttattcgcg tggttgcccg agatggtaca
    17701 cgctacagct acatcgtgtg gatggacacc gaatcgaaat taccgatgag ggttgatctc
    17761 cttgatcgcg atggtgaaac gctggaacaa tttcgcgtga ttgcttttaa tgtcaatcag
    17821 gatatcagca gcagtatgca gacgctggcg aaggcaaatt tgccgccgtt gctttctgtt
    17881 cctgtaggtg aaaaagctaa attcagctgg acgccaacct ggttgccaca gggttttagc
    17941 gaagtttcca gtagtcgacg tccgctaccg acgatggaca acatgcctat tgaatcacgt
    18001 ctctattccg acggattatt cagcttctcg gtaaacgtta accgcgctac gccatcgagc
    18061 accgatcaga tgttgcgcac cggacgcaga accgtcagta caagcgtacg tgataacgcc
    18121 gaaatcacca ttgtcggtga actgccgccg caaacggcga aacgcattgc cgagaatatt
    18181 aagttcgggg cagcgcaatg atcaaagagt gggctaccgt cgtctcctgg gaaaacgggc
    18241 aggcgctggt tagttgtgat gttaaagcct catgcagcag ctgcgcttca cgcgccggtt
    18301 gcggtagccg cgtgttgaat aaacttggcc cgcaaaccac gcataccatt gtcgtaccct
    18361 gtgatgaacc gttagtgccg gggcaaaaag tagaattagg aatcgccgaa ggcagcctgc
    18421 ttagctccgc attactggtt tatatgtcgc cgttggtggg actattcctc atcgcttcac
    18481 tatttcagct actctttgct tcagatattg cagcattatg cggtgcgatt ctcggtggcg
    18541 ttggtggatt cctgattgca cgtggctgct ctcgaaagtt tgctgcccgg tctgaatggc
    18601 aaccgatcat cttaagcgtg gccttgccgc ctggccttgt gcgatttgaa acatcttcgg
    18661 aagacgcgag ccagtgattt cactggcttt gttgcatttt gcataacaaa tgcgaggacg
    18721 tttccagata cccaatccat acggtatgaa catttcctcg cctcaatgtt gtagtgtaga
    18781 atgcggcgtt tctattaata cagacgttaa gctcagaaca gcgacttcta aaagcctggt
    18841 taaccgggca ttaaggcaca ataatcatac tttatgaaga atatacgtaa cttttcgatc
    18901 atagctcaca ttgaccacgg taaatcgacg ctgtctgacc gtattatcca gatctgcggt
    18961 ggcctgtctg accgtgaaat ggaggcgcag gttctcgatt ccatggatct tgagcgtgag
    19021 cgtggcatta ccatcaaagc gcaaagcgtg acgctggact acaaagcgtc tgacggcgaa
    19081 acctatcagc ttaactttat cgacaccccg ggccacgtag acttctccta tgaagtttcc
    19141 cgttcgctgg ctgcctgtga aggtgcattg ctggtggtcg acgccgggca gggcgtagaa
    19201 gcgcaaaccc tggcaaactg ctacaccgcc atggaaatgg atctcgaagt ggtgccggta
    19261 ctgaacaaga ttgacctgcc ggcagccgat cctgaacgcg tggcggaaga aattgaagat
    19321 atcgtcggca tcgatgccac cgacgcggtg cgctgttcag cgaaaaccgg cgttggcgtg
    19381 caggacgttc tcgaacgtct ggtgcgcgac attccgccgc cggaaggcga tccggaaggc
    19441 ccgttgcagg cactcattat cgactcctgg ttcgacaact acctgggcgt tgtttcactt
    19501 atccgtatta aaaacggcac cctgcgtaag ggcgacaaag tgaaagtcat gagtaccggg
    19561 cagacctata acgccgaccg tctgggcatc ttcacgccga agcaggttga ccgtactgaa
    19621 ctgaaatgtg gcgaagtagg ctggctggta tgtgctatta aagatatcca cggcgctcca
    19681 gtaggcgata ccttaacgtt ggcgcgtaat ccggcagaaa aggcgctgcc tggctttaag
    19741 aaagtcaaac cgcaggtata cgccggtctg ttcccggtaa gttccgacga ctatgaagcc
    19801 ttccgtgacg cgctgggcaa actcagcctg aacgatgctt cactgttcta tgagccggaa
    19861 agctccagcg cgctgggctt tggtttccgc tgcggcttcc tcggcctgct gcacatggag
    19921 atcatccagg aacgtctgga acgtgaatac gatctggatc tgattaccac tgcgccgacc
    19981 gtagtgtatg aagttgaaac cacgtcaaga gaagttatct acgttgacag cccatccaag
    20041 ctgcctgcgg taaataacat ttacgaactg cgcgagccga ttgcagagtg tcacatgctg
    20101 ctgccgcagg catatctcgg caacgttatt acgctgtgcg tagaaaaacg cggcgtgcag
    20161 accaatatgg tttaccacgg taatcaggtg gcgctgacgt acgagatccc gatggcggaa
    20221 gtggtgctcg acttcttcga tcgcctgaaa tctacctcgc gtggttatgc gtctctggat
    20281 tacaacttca aacgcttcca ggcgtccgac atggtacgtg tagacgtatt aatcaacggt
    20341 gaacgtgttg atgcgctggc gttgatcacc catcgtgata attcgcaaaa ccgcggtcgc
    20401 gagctggtgg agaagatgaa agatctgatc ccacgccagc agtttgatat cgccattcag
    20461 gcagcgattg gtacgcacat cattgcgcgc tccaccgtga aacagctgcg taaaaacgta
    20521 ctggctaaat gttatggcgg cgatatcagc cgtaagaaaa agctgctgca gaagcagaaa
    20581 gaaggtaaga aacgcatgaa gcagatcggt aacgtcgagc tgccgcagga agcgttcctc
    20641 gccattctgc acgtcggcaa agacaacaaa taacccttag gagttggcat ggcgaatatg
    20701 tttgccctga ttctggtgat tgccacacta gtgacgggca ttttatggtg cgtggataaa
    20761 ttctttttcg cacctaaacg gcgggaacgt caggcagcgg cgcaggcggc tgccggtgac
    20821 tcactggata aagcaacgtt gaaaaaggtt gcgccgaagc ctggctggct ggaaaccggt
    20881 gcttctgttt ttccggtgct ggctatcgta ttgattgtgc gttcgtttat ttatgaaccg
    20941 ttccagatcc cgtcaggttc gatgatgccg actctgttaa tcggtgattt tattctggta
    21001 gagaagtttg cttatggcat taaagatcct atctaccaga aaacgctgat cgaaaccggt
    21061 catccgaaac gcggcgatat cgtggtcttt aaatatccgg aagatccaaa gcttgattac
    21121 atcaagcgcg cggtgggttt accgggcgat aaagtcactt atgatccggt ctcgaaagag
    21181 ctgacgattc aaccgggatg cagttccggc caggcatgtg aaaacgcgct gccggtcacc
    21241 tactccaacg tggaaccgag cgatttcgtt cagaccttct cacgccgtaa tggtggggaa
    21301 gcgaccagcg gattctttga agtgccgaaa aacgaaacca aagaaaatgg aattcgtctt
    21361 tccgagagta aagagacact gggtgatgtg acgcaccgca ttctgacagt gccgattgcg
    21421 caggaccagg tggggatgta ttaccagcag ccagggcaac aactggcaac ctggattgtt
    21481 ccgccgggac aatacttcat gatgggtgac aaccgcgaca acagcgcgga cagccgttac
    21541 tggggctttg tgccggaagc gaatctggtc ggtcgggcaa cggctatctg gatgagtttc
    21601 gataagcaag aaggcgaatg gccgacaggt gtgcgcttaa gtcgcattgg cggcatccat
    21661 taatagccca tcttcgttca cgttgtcgcc gttatggcga caacgtgaat tatttatgag
    21721 ataaatctcc cgtggctaac gacatccccc gtcgttgtgt atagaatatt cccccgaagt
    21781 ttaaggttgg cacctccagg ttgccacggc acacgaaaca gcgttggtcc ccatataccg
    21841 gtaaactgaa actgcagcga agcagttagc agaaccatgt atatcaggtc tgtttcgtgt
    21901 gctgaattgt tgacgcattt atttattggt atcgcatgaa ccccatcgta attaatcggc
    21961 ttcaacggaa gctgggctac acttttaatc atcaggaact gttgcagcag gcattaaccc
    22021 atcgtagtgc cagcagcaaa cataacgagc gtttagaatt tttaggcgac tctattctga
    22081 gctacgttat cgccaatgcg ctttatcacc gtttccctcg tgtggatgaa ggcgatatga
    22141 gccggatgcg cgccacgctg gtccgtggca atacgctggc ggaactggcg cgcgaatttg
    22201 agttaggcga gtgcttacgt ttagggccag gtgaacttaa aagcggtgga tttcgtcgtg
    22261 agtcaattct cgccgacacc gtcgaagcat taattggtgg cgtattcctc gacagtgata
    22321 ttcaaaccgt cgagaaatta atcctcaact ggtatcaaac tcgtttggac gaaattagcc
    22381 caggcgataa acaaaaagat ccgaaaacgc gcttgcaaga atatttgcag ggtcgccatc
    22441 tgccgctgcc gacttatctg gtcgtccagg tacgtggcga agcgcacgat caggaattta
    22501 ctatccactg ccaggtcagc ggcctgagtg aaccggtggt tggcacaggt tcaagccgtc
    22561 gtaaggctga gcaggctgcc gccgaacagg cgttgaaaaa actggagctg gaatgagcat
    22621 cgataaaagt tactgcggat ttattgctat cgtcggacgt ccgaacgttg gcaaatccac
    22681 attgttgaat aaactgttgg ggcagaagat ctccatcacc tcccgcaagg cgcaaacaac
    22741 tcgtcaccgc attgtgggga tccatactga aggcgcgtat caggcgatct acgttgatac
    22801 ccctggcctg catatggaag aaaaacgcgc cattaaccgc ctgatgaaca aagcggcgag
    22861 cagctctatc ggcgacgttg agctggtgat ttttgtcgta gaaggcaccc gctggacgcc
    22921 ggacgatgaa atggtgctca acaaactgcg cgatggcaaa gcgccggtaa tcctcgcggt
    22981 gaacaaagtg gataacgtgc aggaaaaagc cgatctgctg ccacacttac agttcctggc
    23041 aagccagatg aacttcctcg atatcgtgcc gatctctgcc gaaaccgggc tgaatgtcga
    23101 cactatcgcg gcaatcgtgc gtaagcatct gcctgaagcg actcatcact tcccggaaga
    23161 ttacatcacc gatcgctcac agcgttttat ggcgtctgaa atcatccgcg aaaaactgat
    23221 gcgtttcctc ggcgctgaac tgccgtactc cgtgaccgtg gagatcgaac gtttcgtctc
    23281 taacgagcgc ggcggttatg acatcaacgg tttgattctc gttgagcgtg aagggcagaa
    23341 gaagatggtc attggcaaca aaggggccaa gatcaaaacc atcgggattg aagcgcgtaa
    23401 agacatgcag gaaatgttcg aagcgcctgt tcaccttgag ctgtgggtaa aagtgaaatc
    23461 cggttgggcc gacgacgaac gcgcactgcg cagtctcggt tacgttgacg atctttaaga
    23521 gtaactccga tggaaggctg gcagcgcgca tttgtcctgc atagtcgccc gtggagcgaa
    23581 accagcctga tgctggacgt cttcacggag gaatcgggtc gcgtgcgtct ggttgccaaa
    23641 ggcgcacgct ctaaacgctc tactctgaaa ggtgcattac agcctttcac ccctctcttg
    23701 ctacgttttg gcgggcgtgg cgaagtcaaa acgctgcgca gtgctgaagc tgtctcgctg
    23761 gcgctgccat taagcggtat cacgctttac agcggtctgt acatcaacga acttctctcc
    23821 cgcgtactgg aatacgagac gcgcttctct gaactctttt tcgattactt gcactgcatt
    23881 cagtcacttg caggtgccac tggtacgcca gaacccgcgc tgcgccgctt tgaactggca
    23941 ctgctcgggc atctgggcta tggcgtcaat tttactcatt gtgcgggtag cggcgagccg
    24001 gtagatgaca ccatgacgta tcgttatcgc gaagaaaaag ggtttatcgc aagcgtcgtt
    24061 atcgacaata aaacgttcac cggaaggcag ttaaaagcgt taaacgcgcg ggaatttcct
    24121 gacgcagaca cactgcgcgc cgcgaaacgc tttacccgca tggcgcttaa gccgtatctt
    24181 ggcggtaaac cattaaagag cagggaactg ttccggcagt ttatgcctaa gcgaacggtg
    24241 aaaacacatt atgaatgatg aggattgtca tggctgaatt actgttaggc gtcaacattg
    24301 accatattgc aacgctgcgt aacgcacgcg gtactgctta cccagatccg gtgcaggcgg
    24361 cgtttattgc tgagcaggcg ggagcggacg gcattaccgt gcatttacgt gaagatcgcc
    24421 gccacattac cgaccgcgat gtgcgcatcc tgcgtcagac gctggatacc cgcatgaatc
    24481 tggagatggc ggtgaccgaa gagatgctgg cgatcgccgt tgagacgaag ccacattttt
    24541 gctgtctggt accggaaaag cgtcaggaag taacaaccga aggcggcctg gacgtcgctg
    24601 gacagcgtga aaaaatgcgc gatgcctgca aacgtctggc ggaggccggg atccaggttt
    24661 ctctgtttat cgacgccgat gaagagcaga tcaaagctgc ggcagaggtt ggcgcaccgt
    24721 ttatcgagat ccacacaggt tgctatgctg atgccaaaac tgacgccgaa caggcgcaag
    24781 agctggcgcg tatcgccaaa gccgcgacct ttgccgctag tctcggtctg aaagttaacg
    24841 ctggtcacgg tctgacctat cacaacgtga aagccattgc cgccatccct gagatgcatg
    24901 aactgaatat cggtcatgcc attattggtc gtgcagtgat gactggactg aaagatgcgg
    24961 tggcagaaat gaagcgtttg atgctggaag cgcgtggcta atggcaatat taggtttagg
    25021 tacggatatt gtggagatcg ctcgcattga atcggtgatc gcccgatccg gtgagcgcct
    25081 ggcacgccgc gtattaagcg ataacgaatg ggcaatctgg aaaacgcacc accagccggt
    25141 gcgttttctg gcgaagcgtt ttgctgtgaa agaagcggcg gcaaaagcgt ttggcaccgg
    25201 gatccgcaat ggtctggcgt ttaatcaatt tgaagtattc aatgatgagc tcggcaaacc
    25261 acggctacgg ctatggggtg aggcattaaa actggcggaa aagctcggcg ttgtaaatat
    25321 gcatgtaacg ctggcagatg aacgccacta tgcttgtgcc acggtaatta ttgaaagtta
    25381 aaaaaatacc gttctcttta taaagtaaaa cccccgaact ggtaaggaac gggggtttta
    25441 ctttacaact ttaattttgt gcagcacacg attccaacgg aaatgattat ggatcaattt
    25501 agttgacctg tacaagcaac ttgcaatctc tgacataaaa aacgcgagcc tttacgaaga
    25561 aatcgacatt gatgaaaaga ttaacgtgac cgctttttga taagtacatt aaaatcggtt
    25621 tcgttattgg caattttgct gtgctttaca ccatgccact gaattccccc attgaaacga
    25681 gtggtgtcgt caaagctctg gtgtggagtg cagcactcga ttccaataac tcgcacgttc
    25741 agttttgggg tgatgtaagg gctaatctga acggctgcat tccttgttta aggaaaaacg
    25801 aatgactgat tgccgatacc tgattaaacg ggttatcaaa atcatcattg ctgttttaca
    25861 gctgctcctt ttgttcttat aacacaagga aacgtactta aggtgcgtcc ggtgaaccaa
    25921 tcggacgcgc ctttaataac tataaataag tgtctgggca gatactatat aaatttactt
    25981 agtgaatgat tatgctaatt tcatcattta aataaatata atagcgttaa ggtttctcta
    26041 taaaataatt aacactctac ttccagagca gaatattaaa ttttatccgc gtggtgcatc
    26101 agcacaaatt tatcccacaa ctgttcttcc gtctctacat gttgcggatc tttcacaata
    26161 gtattgggga tcgggcacac cttctggcag gttggtgtct cgtagtgccc tacgcactcg
    26221 gtacacttat cgctgttaat ctcgtagata tgatcgccca ttgaaatcgc ctcattcggg
    26281 cattcgggtt cacacatatc acaattgatg cattttttag tgattaacag cgccataagg
    26341 aaactctcaa aacaacacaa accgggcggg cattatacgc gcattcttta ctcagaccag
    26401 ttttttcacc agcgcttcac tatgacgaat acgttctggt gccagttcca gatcctgctg
    26461 aatcagcgca atgaacagca aatccgttaa cattccctgc gcgtgacaag cagagattga
    26521 agcactgttt gtcgcttgtt cttcggcaat ggtatacagg caatgagaag cacgctgttg
    26581 cagggcattg ggagtaaagc cggtaatcgc cagcactttt ccgcccactc gcagcatctc
    26641 atctgccgcc aggtttaact cgcgtcgtac accggtgtag gaaatggcta ataacagatc
    26701 gtcaggggac gacgcctgta ctgtagcgag tagcgcatgc atatcgcgca ctgcggcagc
    26761 attgaagcca atcttcatca gcttccaggc aaagttttgc gccaccagac ccgaagcgcc
    26821 aataccggtc agaattatcc gccgcgcaga gcgcaacatt gttacgcatt catgcagttt
    26881 ctcttcacta ttaacgttta gcgttgcgta catggcggca gtattttctt taatcagttt
    26941 ttcgccgacc agccgtaacg gatcatcacc gcggatttgg ttatgaatgg gcacggaggg
    27001 agattccggc tggcttgcca gcgcttcact caacgccaac ttaagtgccg gaaaaccttt
    27061 atagccgagt ttttgcgcga acttcacgac gctggactga ctgactccgg cttcgttggc
    27121 cagttgctga gagcttaaat ggcgcgccgt atcaggttgt agcagcagat aatccgccag
    27181 ttttttatca ctctgggcaa gcccctggta acgctgacgg atacgaagta agccgttcat
    27241 atctttcctc gcgatgaaat agtggcgaca acaggcaata tgagacaaac gcgagtgaat
    27301 tttatattcc attgccgcta attgccatga attaaaaatt ccagttagcc atgatgcggt
    27361 cgattgttgc cggaaaagtg atgatctatc tgttaggcta tgtagtagcg aaaattaatc
    27421 aggatgtttc agtccagagg agtatggttt ggcaactcac gagcgtcgtg tggtgttttt
    27481 tgacttagat ggaacattgc atcagcagga tatgttcggc agttttttgc gttatttact
    27541 gcgtcgccaa ccgctgaatg cgttgcttgt cctgccgttg ttgccgatca tagccattgc
    27601 gttattgata aaaggccgtg cggcacgctg gccgatgagt ctgcttctgt ggggatgcac
    27661 ttttggtcac aatgaagcgc gtttgcaggc gttgcaggcc gatttcgtac gctggtttcg
    27721 tgacaatgtt accgcctttc cgctggttca ggagcgatta accacctacc tgttaagttc
    27781 cgatgctgat atctggttga ttaccggctc tccgcagccg ctggttgaag cggtttattt
    27841 cgatacgccc tggttgccgc gggttaatct tatcgccagt cagattcagc gtggctatgg
    27901 tggttgggta ttgacgatgc gttgcctcgg gcatgagaag gtcgcgcaac tggagcgcaa
    27961 aatcggcacg ccgctgcggc tgtacagtgg ctatagcgac agtaatcagg acaacccgct
    28021 gctttatttc tgtcagcatc gttggcgagt aaccccgcgc ggtgaactcc agcaactgga
    28081 atagagtaaa gcatagcgtc cgtgtataat gcgccgcgct tttataaccg gagttttctt
    28141 tttgtctgaa gtcgaattta gccacgaata ctggatgcgt cacgcgctga cgctggcaaa
    28201 gcgtgcctgg gatgagcggg aagtgccggt cggcgcggta ttagtgcata acaatcgtgt
    28261 tattggcgaa ggctggaacc gtccgattgg tcgacatgat cccaccgcac atgcagaaat
    28321 catggccctg cggcagggcg gtctggtgat gcaaaattat cgactgatcg acgccacgtt
    28381 gtatgtcacg cttgaaccct gtgtaatgtg tgccggagcg atgatccaca gtcgcattgg
    28441 tcgcgtggtc tttggtgcgc gtgacgcgaa aactggcgct gcgggatctt taatggatgt
    28501 gctgcatcat ccgggtatga atcaccgagt ggaaattacc gaaggaatac tggcggatga
    28561 gtgcgcggcg ttgctcagtg acttctttcg catgcgccgc caggaaatta aagcgcagaa
    28621 aaaagcgcaa tcctcgacgg attaattttg tttctcttca ctccctttcc tggaaaacag
    28681 cagagaggga gagtgggtca aatagtttga tgacgactgg gaaagaaacg agagaaaagg
    28741 aaatttctct ttgcccaact ccgtaggcga taccgccgga taatcctgcg ccagttgcat
    28801 cgccgcttct gtagcctgct tctctttctc ttgcagataa cccaccaggc taatctgata
    28861 cttacgaata ttttcgacat aagcgtaggc ttcatgccca cgagcgtagc cgtaagtcag
    28921 cttgctgtaa tagggtttct ggctaagtaa aggcagacgc tgttttacgt cagcccaact
    28981 gtcaggattc cctttggttt ttgccgtcag agcgcgggca tccagcatat gcgcatagcc
    29041 catattgtac gcggcgaggg caaaccaaat ccgttcgttc tccggcacac tttccggcac
    29101 tttactcatc atatcctgca aataacgcac gccgccgctg atgctctgtt cggcatcggt
    29161 acgatccgta atgccgaggc tttgcgcggt gtttttggtt aacatcatca tgccgcgcac
    29221 acccgtcggt gaagtggcct gagcatccca gtgcgattcc tgataagcaa tagcggccag
    29281 caaacgccag tcaatttctt cggcgtattt ctcaaacagg ggctttaact gcggcagtac
    29341 cgcatcgacg gcgcgtaaaa atgtgcgcgt atcgacgtaa tcaaaatcat cgccatgccc
    29401 caggtatttc tcttcaatgc gtgccagcgt accgtcttca ttcatctcat tgaagaaatc
    29461 gagcagagcg gcggaaaggg tattatcgcc atctaacggg ctaaaccagg tcaccggttg
    29521 ttcatcggtg atatcgagcg ctacggcgag ttccggatga acgcgctgaa acaggctaat
    29581 ggcaacagaa tcagcaatgg tgtaatcgag ttttccttcg atgacatctt ccattaattc
    29641 cgcagagcct tttttgtcgt caaccttcca gcttaattcc gggaattttg tttctttcag
    29701 ggtctggaga tcgttaacca ccacatgacc cggtgcaacg gtgagttgct ccgccgtcag
    29761 gttgcccagt gtacgtgggc gatactgacc cactttataa accagttgtt gtgacacgga
    29821 ataataggta gggccaggct gataattttt tacacgctca ctgttataga ccaggcctgc
    29881 cgccagcagg tcggcgttac cattatcgag gtcgtcaaac agctggctga tattctgccg
    29941 cacggtcact ttcagtttta cgccgaggta atcggcaaac tgtttcgcca gttcgtaatc
    30001 caggccaaaa ggtttcccgt tgatttcgtt atacgtcagg ggagtattaa tggtgctcac
    30061 acgcaactct ccccgcgctt gaatggcggc gatacggttg tcggctttac caaaccaggg
    30121 aatggatggc cagagagcga ccgcgagcag cagtgccaga atgccgatga acagataatt
    30181 aatctttaat tttttcaatt agttaattct ctgtgtcgtg cgcgtcccag cttgaaaaaa
    30241 cgtaataata gtgaaaggtt tactcataaa tgagcggcat tttgcgtaaa cctgcgccag
    30301 ttggcaactt attacagcca ttggcggcac gcgttgctaa ttcacgatgg tgattttatt
    30361 tccacgcaaa cggtttcgtc agcgcatcag attctttata atgacgcccg tttccccccc
    30421 ccttgggtac accgaaagct tagaagacga gagacttatg atggaaattc tgcgtggttc
    30481 gcctgcattg tcggcattcc gaatcaacaa actgctggca cgttttcagg ctgccaggct
    30541 cccggttcac actatttacg ccgagtatgt ccattttgct gacctcaatg cgccgttaaa
    30601 cgatgatgag cacgcacaac ttgaacgcct gctgaaatat ggcccggcac tcgccagcca
    30661 cgccccgcaa ggcaaactcc tgctggtgac cccgcgtcct ggcaccatct ctccctggtc
    30721 ttcgaaagcg accgatatcg cccataattg cggtctacaa caggtaaacc gccttgagcg
    30781 cggcgtggct tactatatag aagccggtac gctgaccaat gaacaatggc agcaggtgac
    30841 cgctgaactg cacgaccgca tgatggaaac ggtctttttt gctttagatg atgcagagca
    30901 gttgtttgct catcatcagc caaccccggt caccagcatt gatttgctgg ggcagggccg
    30961 tcaggcgcta atcgacgcta acctgcgtct tggtctggcg ctggcggaag atgagattga
    31021 ctatctgcag gatgcgttca caaagcttgg tcgtaacccg aacgacatcg agctgtatat
    31081 gttcgcgcag gcgaactccg agcactgccg tcacaaaatt tttaacgccg actggattat
    31141 cgacggtgaa cagcagccga aatcgctgtt caagatgatc aaaaacacct tcgaaaccac
    31201 gccagaccac gttctctctg cttataaaga taacgccgcc gtaatggaag gttctgaagt
    31261 gggtcgctac tttgccgacc acgaaacggg ccgctacgat ttccatcagg aaccggcgca
    31321 tattctgatg aaagttgaaa cccacaacca cccgacggcg atttctccgt ggccgggcgc
    31381 ggcgaccggt tccggcggtg aaatccgcga tgaaggtgcc accggccgcg gcgcgaagcc
    31441 gaaagctggc ctggtcggtt tctccgtatc caacctgcgt attcctggct ttgaacaacc
    31501 gtgggaagaa gatttcggta agcctgagcg cattgtcacc gcgctggaca tcatgacaga
    31561 aggtccgctg ggcggcgcgg catttaacaa cgaatttggt cgtccggcac tgaacggcta
    31621 cttccgtacc tatgaagaaa aagtgaacag ccacaatggc gaagagctgc gcggttatca
    31681 caaaccgatc atgctggcgg gtgggatcgg caacattcgc gccgatcacg tacaaaaagg
    31741 cgagatcaac gttggtgcga agctggtcgt tctcggcggc ccggcaatga acatcggtct
    31801 tggcggcggc gcggcgtctt ctatggcatc tggtcagtct gatgcggacc tcgactttgc
    31861 ttccgtacag cgcgacaacc cggagatgga gcgtcgctgc caggaagtga tcgaccgttg
    31921 ctggcagctt ggtgatgcca acccaatcct gtttatccac gacgttggcg caggcggtct
    31981 ttctaacgcc atgccggaac tggtgagcga cggcgggcgc ggcggtaaat ttgaactgcg
    32041 cgatatcctg agcgacgagc caggcatgag cccgctggag atctggtgta acgaatccca
    32101 ggaacgctac gtgctggcgg ttgctgccga tcagttaccg ctgtttgacg aactgtgtaa
    32161 gcgtgaacga gcaccctacg cggtgattgg cgaggccacc gaagaattac atctttctct
    32221 gcacgatcgt cattttgata atcaacccat cgatctgccg ctggacgtac tgcttggtaa
    32281 aacgccgaag atgacccgcg aagtacaaac gctgaaagcg aagggtgatg cgctggttcg
    32341 tgaagggatt accattgctg acgcggtgaa acgcgtgctg cacctgccga ccgtggcgga
    32401 gaaaactttc ctggtgacca ttggtgaccg cagcgtaacc ggcatggtag cgcgcgatca
    32461 gatggtgggt ccgtggcagg tgccggtcgc taactgcgcg gtcacttccg ccagcctcga
    32521 tagctactac ggcgaagcga tggcgattgg cgagcgtgcg ccggttgcgc tgctggattt
    32581 cgccgcctct gcccgtctgg cggtcggtga agcgttaacc aatattgccg caacacagat
    32641 tggcgatatc aaacgcatta aactttccgc caactggatg gcggcggcag gccaccctgg
    32701 tgaagatgcg ggcctgtatg aagccgttaa agctgtgggc gaagaacttt gtccggcgct
    32761 gggcctgacg atcccggtgg gtaaagactc tatgtcgatg aaaacccgct ggcaggaagg
    32821 taacgaagag cgcgaaatga cgtcgccgct gtcgctggtg atttctgcat ttgcccgtgt
    32881 ggaagatgtg cgtcacacca tcacgccgca gctttctacc gaagataacg cattactgct
    32941 gattgatctg ggcaaaggca ataacgcgct gggcgcaacg gcgctggcgc aggtttatcg
    33001 tcagcttggc gacaaaccgg cagatgtacg cgacgttgcc caactgaaag gcttctatga
    33061 cgcgattcag gcgctggttg cacagcgtaa gctgctcgcg taccacgacc gctcggatgg
    33121 cggcctgctg gtaacgctgg cggaaatggc gtttgctggt cattgtggca ttgacgcaga
    33181 tatcgcctct ctgggtgacg atcgcctggc tgcgttgttt aacgaagaac tgggtgcggt
    33241 gattcaggtt cgtgccgctg accgtgaagc ggtcgagtcc gtactggcac agcatggact
    33301 tgcggattgc gtccattatg tagggcaggc ggtttccggt gaccgttttg tgattaccgc
    33361 caacgggcag actgtattca gcgaaagccg cactacgttg cgtgtctggt gggcagaaac
    33421 tacctggcag atgcagcgcc tgcgtgacaa cccggagtgt gccgatcagg aacatcaggc
    33481 gaaatctaac gacgccgatc cgggcctgaa tgtgaaactg tcgtttgata tcaacgaaga
    33541 tgtggcagca ccgtatattg ccaccggtgc gcgtccgaaa gttgccgtgc tgcgtgagca
    33601 gggtgtgaac tcgcatgttg aaatggcggc agctttccac cgtgcaggct tcgatgcaat
    33661 cgatgtgcat atgagcgacc tgctggctgg acgcacggga ctggaggatt tccacgccct
    33721 ggtcgcgtgc ggtggtttct cctacggtga tgtactgggt gccggtgaag gttgggcgaa
    33781 gtctatcctg ttcaatgacc gtgtacgcga tgagtttgca accttcttcc accgtccgca
    33841 aacgctggcg ctgggggtat gtaacggttg ccagatgatg tctaatctgc gcgaactgat
    33901 cccaggcagt gagttgtggc cacgttttgt gcgcaatacc tccgatcgct ttgaagcgcg
    33961 tttcagcctg gttgaagtaa cccaaagccc gtctctgctg ttgcaggaga tggtgggctc
    34021 gcaaatgccg attgctgtct ctcatggtga agggcgcgtg gaagtgcgtg atgcggcgca
    34081 tctggcggcg ctggaaagta aagggctggt ggcactgcgc tatgtcgata acttcggcaa
    34141 agtcactgaa acctacccgg ctaacccgaa cggttctccg aatggtatta cggcggtcac
    34201 gactgaaagt ggtcgagtca ctattatgat gccgcacccg gaacgtgttt tccgtactgt
    34261 cagcaactcc tggcatccgg aaaactgggg cgaggatggc ccatggatgc gcattttccg
    34321 caatgcacgt aagcagttgg ggtaagccgt caaactcatt gtttttgcag cccggatgag
    34381 gcattcacgc cgcatccggc attaagcacc aaccgctatt ataagctccc aacttcggga
    34441 gcttttttgt gtctgtaaat cacgacaatg ggtggtttac cgtgtcgctt tctggcgaca
    34501 cttaactgat tgattttaat attatctaat aagtttatct taagttgtct ctttttagcg
    34561 acacagtggc tgatttacat tcaaatagcg aagaagccat aaatccctca aagcagcgta
    34621 taatcagtac gttaccaaac tattttcttt attggcacag ttactgcata atagtaacca
    34681 gtggctcatt caccgactta tgtcagcccc ttcgggacgt gctacataaa attcgaatga
    34741 cgcacaacaa ggtgcctgcc gtccaacttc tgatatcagc gtagctatat caaccctcgg
    34801 gcgaaacgtc gagttaggca ccgccttatt ccataacaaa gccgggtcat tcccggcttt
    34861 gttgtatctg aacttcccct cggttagcat caggctattc gcgtctgacg agagtaacac
    34921 cttgaaacgc tggcccgttt ttccccgctc attacgacaa ctggtaatgc tggcattttt
    34981 gctgattctg ctacccctgt tggtgctggc atggcaagcc tggcaaagcc tgaatgcgct
    35041 tagcgatcag gcggcgctgg ttaaccgcac tacgcttatt gatgcccggc gcagtgaagc
    35101 gatgaccaac gcggcgctgg agatggagcg tagctaccgt cagtattgcg tgttggacga
    35161 cccaaccctg gcgaaggttt atcaaagcca gcgcaagcgt tacagcgaaa tgctcgatgc
    35221 ccacgcaggc gtgctgccgg acgataaact ctaccaggca ttacgtcagg acttgaacaa
    35281 tctggctcaa cttcagtgta acaacagcgg tcccgatgcc gccgccgccg cgcgtctgga
    35341 agcctttgcc agtgccaata ccgaaatggt gcaggccacg cgcacagtgg tgttctctcg
    35401 tgggcagcaa cttcagcgtg aaatcgccga acgtgggcaa tattttggtt ggcaatcgct
    35461 ggtgctattt ctggtgagtc tggtgatggt gctacttttc acgcggatga ttatcgggcc
    35521 ggtgaaaaat atcgagcgca tgatcaaccg gctgggggaa gggcgttctc tgggcaatag
    35581 cgtctcgttc agtggaccga gcgagttacg ctcggttggg caacgtattc tttggttaag
    35641 tgagcgcctg tcatggctgg aatcccaacg ccatcaattt ttaagacatt tatctcatga
    35701 attaaaaacc ccgctggcga gtatgcgcga gggcactgaa ttactggcag accaggttgt
    35761 cgggccgctt acgcccgagc aaaaagaggt ggtgagcatc cttgatagca gcagccgcaa
    35821 tttgcaaaaa ctgatcgaac aactgcttga ttacaaccgt aaacaagcgg acagtgcggt
    35881 ggaactggag aatgttgagt tagcaccgct ggtggagaca gtagtttctg ctcatagcct
    35941 gcccgcacgg gctaaaatga tgcataccga cgttgatctc aaagcaacag cttgcctggc
    36001 ggagccaatg ctgctgatga gcgtactgga taatctttac tccaatgcgg tgcactacgg
    36061 ggctgaatcc ggtaacattt gccttcgcag cagtttacat ggtgcgcggg tttatattga
    36121 tgtcatcaat acaggcacgc ccattccgca agaggaacgc gccatgatct tcgaaccctt
    36181 ttttcaggga agccaccagc gaaaaggggc ggtgaagggc agcggtctgg gattaagcat
    36241 tgccagggat tgtattcgcc gtatgcaagg ggaactgtat ctggtcgacg agagcgggca
    36301 agatgtttgt ttccgcattg aattaccgtc gtcgaaaaac acgaaataat gctaacagcc
    36361 tatcccatca ggctatttta tttgccagtt tggccccggg cagtgctcga aatcttcacg
    36421 tacgacatgt acgctccggt ttctccgcgc tgtccatgtc caaactgcct gcaacaatta
    36481 cgcccactgg gataggcttt aagtctggtg aatatgcgac acatttttca acgattattg
    36541 ccccgacggc tgtggctggc agggctgcca tgtctggcat tgctgggttg cgtgcagagt
    36601 cacaataagc cagccattga tacgccagca gaagaaaaaa ttccggttta tcaactggct
    36661 gactatcttt ctactgaatg tagcgacatc tgggcgctgc aaggtaaatc aaccgaaacc
    36721 aatccgcttt actggctgcg ggcgatggat tgtgctgatc gtttaatgcc tgcgcagtcg
    36781 cgtcagcaag cccgccaata tgacgacggc agctggcaaa atacctttaa acaagggatt
    36841 ttgctcgccg acgccaaaat tacgccgtat gagcgccgcc agctggtcgc gcgcattgat
    36901 gcgctaagta ccaagatccc ggcacaggtt cgccctctgt atcaactctg gcgcgacggt
    36961 caggcgctgc aactgcaact ggcggaagag cgccaacgct acagtaaact ccagcagtcc
    37021 agcgacagcg agctggatac tttgcgccag caacatcacg ttctacaaca gcaactggaa
    37081 ctcaccaccc gcaagctgga aaacctgact gatattgaac gccagctctc gacccgcaaa
    37141 ccggctggaa atttctcgcc ggatacgcca cacgagagtg aaaagcccgc gccatccacc
    37201 gatgaggtca ctcctgatga gccataaacc tgcgcattta ttattggtcg atgacgatcc
    37261 gggattgctg aaactgcttg gcctgcgtct gaccagcgaa ggctacagcg tggtcacagc
    37321 ggaaagtggc gctgaaggat tacgggtact gaatcgcgaa aaagtagatt tagtcatcag
    37381 cgacctgcgg atggatgaaa tggacggtat gcagctgttt gctgaaatcc agaaagtgca
    37441 gccgggaatg ccagtaatta ttcttaccgc gcatggttct attcctgatg ccgttgctgc
    37501 aacacagcag ggcgtgttca gttttctcac caagcctgtc gataaagatg cgctatatca
    37561 ggcaattgac gatgcgctgg aacaatccgc gccagccacc gacgaacgct ggcgcgaggc
    37621 gattgtcacc cgcagcccgc tgatgctgcg cttgctggaa caggcgcggc tggtggcaca
    37681 atcagacgtt agcgttttga ttaacggcca gagcggcacc gggaaagaga ttttcgccca
    37741 ggctatccac aacgcgagcc cgcgcaacag caaaccattt attgctatta actgtggcgc
    37801 attgcccgag cagttgctgg agtcggagct gtttggtcat gcacgtggcg cgtttactgg
    37861 cgctgtcagc aatcgcgaag gtttattcca ggcggcggaa ggcggtacgc tatttcttga
    37921 tgagattggc gatatgcctg caccgttgca ggtcaaactg ctgcgcgtgt tgcaggagcg
    37981 taaagtgcgc ccgctgggca gtaaccgcga tattgatatc gacgtgcgga ttatttctgc
    38041 cactcaccgc gacctgccaa aagcgatggc gcgcggggaa ttccgcgaag atctctatta
    38101 ccgcctcaac gttgtcagcc tgaaaattcc ggcactggcg gagcgcacag aagacattcc
    38161 gctactggca aatcacctgt tgcgccaggc ggcagagcga cataaaccgt ttgtccgcgc
    38221 gttctccacc gatgcgatga aacgcctgat gaccgctagc tggccgggta atgtgcgcca
    38281 gttggtcaac gtgattgaac agtgcgtggc gctgacctca tctccggtga ttagtgatgc
    38341 gctggtggag caggcgctgg agggtgaaaa tacggcgctg ccaacctttg ttgaggcacg
    38401 taatcagttt gaactcaact atttgcgtaa gctgctgcaa attaccaaag gcaacgtcac
    38461 ccacgcggcg agaatggcgg ggcgcaaccg gacagaattt tataaactgc tttcccgaca
    38521 cgagctggat gcaaacgatt tcaaggaatg aattggcgtt atgtgttacg tttagcagat
    38581 caaaagacag gcgacctttt caaggaatag catgaaaaag attgatgcga ttattaaacc
    38641 cttcaagctg gacgatgttc gcgaagcact ggctgaagtc ggcattaccg gcatgacggt
    38701 cactgaggtg aaaggttttg gccgccagaa aggccatacc gagctgtacc gcggcgcgga
    38761 gtatatggtg gattttctgc cgaaagtgaa aattgagatt gtcgtaccgg acgacattgt
    38821 cgatacctgt gtcgatacca ttattcgcac ggcgcaaacc ggcaaaatcg gtgacggtaa
    38881 aatcttcgtc tttgacgtgg cacgggtcat tcgtatccgt accggtgaag aagacgacgc
    38941 ggcaatttaa accgcgtcaa gggttgccgg atgtttccat ccggcaacat caaatcacag
    39001 caccttatgc gggccaaagc attcgtaatg aatgttttcc tgcttcacgc ccagatccac
    39061 taactgtttc gcggcaaact gcatgaagcc aaccgggccg cagagataga actgcattgt
    39121 cggatcgctg aacgcacctt ccagtttgct caaatccatc agaccttcgc tatcaaactg
    39181 acctttagcg cgatcggctt cgcttggctg acgataccag gtgtgcgcgg taaagcgcgg
    39241 cagtgcttgc cccagttcct taacttcatc ggcaaaggcg tgaacatcgc cattttctgc
    39301 cgcatggaac cagttcactt gtgctgtgtg gccagctttt gccagcgtgt cgagcattgc
    39361 cagcattggc gtttgaccga caccggcaga gattaacgtc actggtgtgt catctgcgac
    39421 agccataaag aaatcacctg ctggagcaac cagtttcacg acatcgccaa cattggcgtg
    39481 attgtgcagc cagttggata cctgcccgcc atcttcgcgt ttcaccgcaa tacgatagcc
    39541 tttgccatcc ggtttgcgag tcaaagagta ctgacgaatt tcctgatgcg ggaaaccttc
    39601 cggcttcagc cagacgccga gatattgccc cggacggtat tctgccactg cgccaccgtc
    39661 gaccggctcc agttcgaagc tggtgataag cgcgctgcgc ggtgttttag ccacaatgcg
    39721 gaaatcgcga gtaccttccc agccaccggc tttgctggcg ttttcgttat agatttccgc
    39781 ctcgcgattg ataaatacat tagccaggac gccataggct ttaccccacg cgtccagcac
    39841 ttcctgcccc gggctgaaca tttcgtccag cgttgccaac aggtgttcac cgacgatgtt
    39901 gtactgttcc ggtttgatct ggaaactggt gtgcttctgc gcgatttttt ctaccgctgg
    39961 cagcagcgca ggcaggtttt caatattgct ggcgtaggcg gcaatagcgt taaacagggc
    40021 ttcacgttga tcgccattgc gctggttact catgttaaaa atttctttga gttctgggtt
    40081 atgagtaaac atacggtcgt agaaatgggc ggttaacttt ggtcccgttt ccaccagtaa
    40141 agggatggtg gcttttactg tagcgatggt ttgagcgtca agcatatggt cttccttttt
    40201 ttgcatctta attgatgtat ctcaaatgca tcttataaaa aatagccctg caatgtaaat
    40261 ggttctttgg tgtttttcag aaagaatgtg atgaagtgaa aaatttgcat cacaaacctg
    40321 aaaagaaatc cgtttccggt tgcaagctct ttattctcca aatccttgcg tagcctgaag
    40381 gtaatcgttt gcgtaaattc ctttgtcaag acctgttatc gcacaatgat tcggttatac
    40441 tgttcgccgt tgtccaacag gaccgcctat aaaggccaaa aattttattg ttagctgagt
    40501 caggagatgc ggatgttaaa gcgtgaaatg aacattgccg attatgatgc cgaactgtgg
    40561 caggctatgg agcaggaaaa agtacgtcag gaagagcaca tcgaactgat cgcctccgaa
    40621 aactacacca gcccgcgcgt aatgcaggcg cagggttctc agctgaccaa caaatatgct
    40681 gaaggttatc cgggcaaacg ctactacggc ggttgcgagt atgttgatat cgttgaacaa
    40741 ctggcgatcg atcgtgcgaa agaactgttc ggcgctgact acgctaacgt ccagccgcac
    40801 tccggctccc aggctaactt tgcggtgtac actgcgttgc tggaaccagg cgataccgtt
    40861 ctgggtatga atctggcgca tggcggtcac ctgactcacg gttctccggt taacttctcc
    40921 ggtaaactgt acaacatcgt tccttacggt atcgatgcta ccggtcatat cgactacgcc
    40981 gatctggaaa aacaagccaa agaacacaaa ccgaaaatga ttatcggtgg cttctctgca
    41041 tattccggcg tggtggactg ggcgaaaatg cgtgaaatcg ctgacagcat cggtgcttac
    41101 ctgttcgttg atatggcgca cgttgcgggc ctggttgctg ctggcgtcta cccgaacccg
    41161 gttcctcatg ctcacgttgt tactaccacc actcacaaaa ccctggcggg tccgcgcggc
    41221 ggcctgatcc tggcgaaagg tggtagcgaa gagctgtaca aaaaactgaa ctctgccgtt
    41281 ttccctggtg gtcagggcgg cccgttgatg cacgtaatcg ctggtaaagc ggttgctctg
    41341 aaagaagcga tggagcctga gttcaaaact taccagcagc aggtcgctaa aaacgctaaa
    41401 gcgatggtag aagtgttcct ggaacgcggc tacaaagtgg tttccggcgg cactgataac
    41461 cacctgttcc tggttgatct ggttgataaa aacctgaccg gtaaagaagc tgacgccgct
    41521 ctgggccgtg ctaacatcac cgtcaacaaa aacagcgtac cgaacgatcc gaagagcccg
    41581 tttgtgacct ccggtattcg tgtaggtact ccggcgatta cccgtcgtgg ctttaaagaa
    41641 gccgaagcga aagaactggc tggctggatg tgtgacgtgc tggacagcat caatgatgaa
    41701 gctgttatcg agcgcatcaa aggtaaagtt ctcgacatct gcgcacgtta tccggtttac
    41761 gcataagcga aacggttaat ctgttgaaaa aaggcttcct tcgggaagcc tttctacgtt
    41821 attgcgccat caaatctgcc gtaactgcgc ctcaacatac aaatagccaa ttcccagcac
    41881 ctgctgtgcg cggcttaatt gcccaaagcc aatttgcgtc gctttcacgc tcggtccttc
    41941 gtcgcggtcg aacgggttaa atcccgtctg gcgaataatg gtattaagcc agttctcacc
    42001 aaaggcacaa ctgcgaccgt acaaccaaat ctgattaata ttgaggatgt tgaggaagtt
    42061 atacagactc aaaccaatgg cgttggcaga gcgatcaacc caactggtga tccacggttc
    42121 tccactctgc caggcagcga ttaactgcgc ggtagtcagt ttttcaggat caagctgagt
    42181 attaaccggt tgtgatttta gccatacccg cgcctgtttt tttaatgcgc ttaacgaggc
    42241 gacggtttcc aggcagccgt aacgcccgca gtcgcagacg acgccatccg gattaacgat
    42301 ggtgtgacca atttgcccgc taccatacaa actgccgcga taaatttgct cgttaatcac
    42361 gaacgacgag ccaatgccgt aatcaacgtt gatcacgcag aaatcccgtt cctgcgaatt
    42421 attttgccat ttctccgcca gcgccagcat cacgcagtca ttatcgacca tcacccgaat
    42481 gccgagcttc tcttccagca gatactttac ctcaaccggc gttgtccacg gcgcttgcgg
    42541 catggtttgc gacacgccag tcaccggatc aacctgaccg tggattgcca gcgccaggtt
    42601 gatggtacgg tccggccaca atttacggtg gcgatgccag catttttcga tttcggacag
    42661 cagcgcctgc ggcgtcggtg catcaatctg taaatactca aattcacctt tcggacttaa
    42721 acaggcgtta gcgacctgac actcaatact ggtgggcgtc acgttcaggc acagcgtcca
    42781 gtcaccttcc ggcgcaatca gccatgtacc gctactatgc ccgcgcgtct ggctttcatc
    42841 gtcgatattt accacccgtt tttcgctttc cagttcctgc aaaatattac tgactgccgg
    42901 aatcgaaatt tgcgccagcc gggccagcgt tgatttattg gcgcgctttt gccggtacag
    42961 cagttccaga atcacgcatt tgttatggtg gcgaatctgt tgattattaa tgcaggctct
    43021 catcttcact aaaccttgtt tagttaattg cgtgattatt ccgcgaaaac attgccagat
    43081 tgttcaatac actgccacaa atcttttaat tcagtatgtc ttgttaatat tgagggcacc
    43141 atgactccag taaaagtgtg gcaagagcgc gttgagatcc cgacctatga aaccgggccg
    43201 caggatatac atcccatgtt cctggaaaat cgcgtttatc agggatcgtc cggcgcggtt
    43261 tatccctacg gcgtgaccga tacgctgagc gagcagaaaa ccctgaaatc ctggcaggcg
    43321 gtgtggctgg aaaatgacta catcaaagtg atgatcctgc cggaactggg cggtcgggtg
    43381 catcgcgcat gggataaagt gaaacagcgc gattttgtct atcacaatga agtcattaaa
    43441 cctgcgctgg tggggctgct gggaccgtgg atctccggcg ggattgagtt taactggccg
    43501 caacaccatc gcccgaccac ctttatgccc gttgatttca ccctcgaagc ccatgacgac
    43561 ggcgcacaga cggtgtgggt cggcgaaacg gagccgatgc acggtttaca ggtgatgaca
    43621 ggtttcaccc tgcgtcctga ccgggcggcg ctggaaatcg ccagtcgcgt ctataacggc
    43681 aacgccacgc cgcgtcattt cttgtggtgg gccaacccgg cagtgaaagg gggtgaaggg
    43741 catcagagcg tcttcccgcc ggatgtaacg gcggtgtttg atcacggcaa acgggccgtc
    43801 tccgctttcc ccatcgccac cggcacttac tacaaagtgg actactccgc cggagtggac
    43861 atttctcgct ataaaaatgt gcccgttcca acctcatata tggctgaaaa atcacagtac
    43921 gattttgttg gcgcgtggtg tcacgatgaa gatggtggtt tgctacacgt tgccaaccac
    43981 catattgcgc caggtaaaaa acagtggagc tggggacaca gtgaatttgg ccaggcgtgg
    44041 gataagagcc tgactgacaa taacggcccg tatatcgaac tgatgaccgg tatttttgcc
    44101 gataaccagc ctgattttac ctggcttgat gcttacgagg agaagcgttt cgagcagtat
    44161 ttcctgcctt atcattctct gggcatggtg caaaatgcct cccgcgatgc ggtgataaaa
    44221 ctccagcgta gtgagcgggg gattgagtgg gggctgtatg ccatctctcc gttgaacgga
    44281 taccgcctgg cgatccgcga aatcggcaaa tgcaacgcgt tgctcgatga tgccgtggcc
    44341 ctgacaccag cgaccgccat ccagggcgtg ttgcacggta tcaatcctga cagactgacc
    44401 attgagctct ccgatgccga cggcaatatt gtactgagtt atcatgaaca tcagccgcaa
    44461 gcgttgccgt tgccggacgt cgccaaagcg ccactgtcag cacaagacat taccagtaca
    44521 gatgaagcct ggtttatcgg tcagcatctg gagcaatatc atcacgccag ccgttcaccg
    44581 ttcgattact acctgcgcgg cgtggcgctg gatccgctgg attatcgctg taacctggcg
    44641 ctggcgatgc tggaatataa ccgcgccgat ttcccgcaag cggtggcgta tgccactcag
    44701 gcgctgaaac gcgcacatgc gctgaacaaa aatccgcagt gcggacaggc cagtttgatt
    44761 cgcgccagtg cttacgaacg tcagggacaa tatcaacaag ccgaagagga tttctggcgg
    44821 gcggtctgga gcggcaatag taaagcaggt ggctattatg gtctggcacg actggcggcg
    44881 cgtaatggtc acttcgatgc tggtctggat ttttgccaac aaagtcttcg cgcctgccca
    44941 accaatcagg aagtgctttg cctgcataat ctgctgctgg tgttaagtgg tcgtcaggac
    45001 aacgcgcgtt tgcagcgcga gaaactgctg cgcgattatc cgctgaacgc cactctgtgg
    45061 tggctgaact ggttcgatgg tcgtagcgaa tcagccctcg cgcagtggcg cggtctgtgt
    45121 cagggacgcg acgttaacgc cctgatgacc gccgggcaac tgattaactg gggaatgcct
    45181 accctggcgg cagagatgct gaacgcactg gactgccagc gcacgctgcc gctttatctg
    45241 caggcgagtt tattgccgaa agccgaacgc ggcgaactgg tcgcaaaagc cattgatgtc
    45301 ttcccgcagt ttgtccgttt cccgaatacg ctggaagaag tggcggcgct ggagagtatt
    45361 gaagagtgct ggtttgcccg tcatttattg gcctgcttct actacaacaa gcgtagctac
    45421 ggcaaagcca ttgccttatg gcaacgttgc gtagagatgt cgccggagtt tgccgacggc
    45481 tggcgcgggt tagcgatcca tgcgtggaat aagcaacacg attatgagct ggccgcgcgt
    45541 tatcttgata atgcttatca gcttgcgccg caggatgcac gtctgctttt cgaacgggat
    45601 ttgctggata agttaagtgg agccacaccg gagaaacgac tggcgcgtct ggaaaataat
    45661 ctggaaattg cgctgaaacg cgacgacatg accgcagaac tgctcaattt gtggcatctc
    45721 acgggtcagg cagacaaagc ggcggacatt ctcgccacgc gcaaattcca cccgtgggaa
    45781 ggcggggaag ggaagatcac cagtcagttt atcctcaacc agttattacg cgcctggcag
    45841 catcttgatg ccagagagcc gcagcaggcc agcgaactgc ttcatgccgc gctgcattat
    45901 ccggagaatt taagcgaagg ccgtttaccg gggcaaactg ataacgacat ctggttctgg
    45961 caggcgatat gcgccaacgc gcagggagat gaaactgaag cgatgcgttg tttacgtctg
    46021 gcggcgactg gcgatcgcac cattaacatt cacagttatt acaacgatca gccggttgat
    46081 tatctcttct ggcaaggaat ggcgctgcga ctgctgggcg aacaacaaat cgcacagcaa
    46141 ctgtttagtg aaatgaaaca gtgggcgcaa gagatggcga aaaccagtat tgaggcggat
    46201 ttctttgctg tttcacaacc tgacctgttg tcgctgtatg gcgatttaca acagcagcat
    46261 aaagaaaaat gcctgatggt ggcgatgctg gcgtccgcgg gactcgggga ggttgcgcac
    46321 tatgaatctg ctcgcgctga attgacggcg attaatccgg cctggccgaa agcggcatta
    46381 ttcaccactg tgatgccttt tatttttaac tacgttcact aaaccctatt aactgtaata
    46441 tttgagcggc acgcgctgcc gctcacgctc aaataattaa aataagccgt tgtaaataaa
    46501 aaccctacag gaggcactat gcctaaaaaa atgagaacaa cccgtaatct attattaatg
    46561 gctacgctat taggtagcgc actatttgcc agggctgcgg ataaagaaat gaccattggc
    46621 gcaatatacc ttgataccca gggatattac gctggcgtgc gtcagggtgt tcaggatgcg
    46681 gcaaaagatt cttcagtaca ggtacagtta attgaaacta acgcccaggg tgatatttcg
    46741 aaagaaagta cctttgttga taccctcgtg gcgcgtaatg tcgatgccat tattttatcg
    46801 gcagtgtctg aaaatggcag tagccgtacc gttcgtcgcg ccagtgaagc gggtattccg
    46861 gtgatttgct acaacacctg tattaatcaa aaaggcgtcg ataaatatgt ctcggcgtat
    46921 ctggtcggcg atccgctgga atttggtaaa aaactgggta atgctgccgc cgattatttt
    46981 attgccaaca aaattgacca gccgaaaatt gccgtcatca attgcgaagc ctttgaagtt
    47041 tgtgtgcagc gacgtaaagg gtttgaagaa gtattaaaag cccgcgttcc cggcgcgcaa
    47101 attgtcgcta atcaggaagg gactgtttta gataaagcga tttccgttgg tgaaaaacta
    47161 attatctcca ggccggatct caacgccatt atgggtgaat cgggcggagc gacactcggc
    47221 gcggtaaaag tggtacgtaa tcaaaatcag gccggaaaaa ttgctgtttt cggttcggat
    47281 atgatcactg aaattgctca ggagctggaa aacaatcagg tgctgaaagc ggtagtggat
    47341 atttccggca agaaaatggg caatgctgtt ttcgcgcaaa cattgaaggt tatcaataaa
    47401 caagccgacg gtgaaaaagt gattcaggtg cctatcgatc tctataccaa aacggaagac
    47461 ggtaaacagt ggctggcaac gcacgttgat ggtctgccct aatcgctgat tcgcagaggt
    47521 ccctatgttc acggc
//
LOCUS       DAMZFT010000019        45465 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.019, whole genome shotgun sequence.
ACCESSION   DAMZFT010000019 DAMZFT010000000
VERSION     DAMZFT010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 45465)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45465)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45465
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.019"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            392..3667
                     /locus_tag="PN977_001246"
     CDS             392..3667
                     /locus_tag="PN977_001246"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6041301.1"
                     /translation="MKKNSLNVILDTVFSAKSINGEKSHSFISYKLVKAVENLSGYDL
                     DLFLNRAINHPNFPSNMDFSLRTVFTIEQSNTIEFKNFISELVWYKHIFIRYKKHLND
                     ILSAKARLEKLVLFATGSECIKYLDEIESSYGVSFWSIDARLLINKVLLNESNGLYVK
                     SILSKTHYKLTEFMLQQLLFKHQVHNFDDFSKNLIKILDDMRTTQDNGYARNLADGIS
                     SFLIPLEFDKNIDLKNRTLAPFTNLPLIDQFIIFQRTISDLQLHGNGLRNSELSLIAE
                     INSFIKNNYLNNILDGRERNIENIDEHYKSVIRRYTIGDYTGCISEINSVRTQKTILP
                     LIEIYAKSHVYLDKKISDSCIFNKLTNWLIAIIKCDRRAGKYIDEFELLASKIYFNSN
                     SSSLFFTLYNLISDNHNKLNISRINLTKNGSFVTSLHLNITIKELWTELGIKENEIPK
                     YRLFKFKDLANLSFDEIKKHYEVYNDNVIIRSEYLKDYTNFLLENEKYELCIQFIAEN
                     CIGNPANYYYFPIRKIIDFVKDNIFDYASVALSILIDIFVKSTSNTSNELLLESYESL
                     VDISNINRPSTHYCSKQLTPLEHYFLKFICIPSVMDSDPSFTGTDDLKKERIAIIDLL
                     LKEHDDADLLKEKDEIIDEISFEEIKTKYETGKIFVDIENLKRAKLERYRYYFDALKD
                     SLILGLEPPEDFALITDDGNITAIPSGDTNSIIHELLKELISDFVKNENYGLDKYLSA
                     DIRHGVFENQLRSSAEKSQLITDMDGTGQYSKSNLIIETYPLINPIINNEIGTAIADF
                     SYHFDIELAKANSWFNVKTMLISESQQGMIDFLISVDMFNSFKAAVSNQASFEKFFDA
                     CINFMWERTFRCLNDIKERLHYEFKRNILDLIATLRHQIDIYRRRSSMREIQEKIDLL
                     AESINKEIATVCDWLNVLEYNEEKIYKISSVMQACRKTFFNIHHCSDDAIVFDSMYHD
                     DEPKLSYKEAKPLITSIITALNNAMAYGNKKIFISIKPEEKSWKITIRNLIIETKNRT
                     TNQILQEIDDKIKRGDNSLNIKEGGAGIYKIYDLLCSLPQRFNVNHCIQNNEFILNIE
                     IKK"
     gene            3664..4113
                     /locus_tag="PN977_001247"
     CDS             3664..4113
                     /locus_tag="PN977_001247"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HDI6041302.1"
                     /translation="MKILLVEDIEYKRDKVIGLLESISADVSVDVAKSYVSAVNSATS
                     KTYDLIILDMSLPTYDKGPNENGGRFRVYGGKDIIRKLMRGKEHPVVVVLTQHTTFGE
                     IGEQKNTDTLSQELNALMGSKFIGIVKYDSTKPQWKDEIKKVVQDLL"
     gene            4110..5273
                     /locus_tag="PN977_001248"
     CDS             4110..5273
                     /locus_tag="PN977_001248"
                     /inference="COORDINATES: protein motif:HMM:NF017773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDI6041303.1"
                     /translation="MIKILIVDDNKSRIEKLKSSLTELITKNMIRIDEKYTSDAAKIA
                     LKLNQYDYLILDVFLPKKDNYSPDERNGLGLLKQINSDSKFYSPKKIVGITAYLNDIS
                     RYESEFREYASIIFEARRNDTGWLESLKKIIEKDVESQVSYNLNEKDSVLITVHGIRT
                     YAPWQNTIEEKITNISNKFNYIKFNYGFLNILCFLFPPTRHLFARKIIQDIRITVESN
                     KNKRIYIICHSFGTYLVYCALSKLTHTDAKIECLIFSGSVLKRTTSLKTLKQHCNAII
                     NDCAVSDYILLLCKMLVIGLGDAGRKGFIEPNDGVFINRYFKGGHSTYFEDKDFIEVN
                     WLPLIFDNKNIASRDERKNHIFSDVTNALQNIIEYLKIPIWLFFSFLLIALVL"
     gene            complement(5753..6718)
                     /locus_tag="PN977_001249"
     CDS             complement(5753..6718)
                     /locus_tag="PN977_001249"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6041304.1"
                     /translation="MTYENFHSDLTNILNGEYEKEIHDWDKIKAVLLHIVKNNYQGFG
                     RNIVDFIDRGSWDRITKIDFKDGNRQLELTWNNGWLYHASIETILIIEHERAFFVLIK
                     SYYQDRKKLNKLYSARCRSYEIDKFGHYMVEVQRVTRSGEEFIQIPNINCYTTAIMIR
                     PPNRVPVSNHASELLMHNINLNLAIAKFDFLLQELSDIKEYDRDALQEKGNTARRYLE
                     YVLMLVNIRAEKEFEEDYQKLMLGSLSRVINFLGLPNKLKNDITLAQELLNSCSHHGG
                     VRIEKNELEQAMETLQQLCQWIKGIDFFKVSKDINGKSININKPF"
     gene            complement(6820..8004)
                     /locus_tag="PN977_001250"
     CDS             complement(6820..8004)
                     /locus_tag="PN977_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008783799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDI6041305.1"
                     /translation="MKLNARQVDAAKPREKAYKLADGAGLYLEVVPSGSRYWRMKYRF
                     NGKEKRMAFGVYPAVSLAQARALRDEAKKKLAEGIDPSFAKKEEKLVRDVQLNNTFQA
                     VALEWHGTKVSRWSEGYASDIIEAFNKDIFPYIGQLPVNDIKPLVLLNVLRRMESRGA
                     TEKAKKVRQRCSEVFRYAIVTGRAEYNPAADLTSAMSGHESKHYPFLTVEELPDFFKA
                     LAGYTGSPLVVLAARLLILTGVRTGELRGAFWSEFDLEKAVWEIPAERMKMKRPHLVP
                     LSTQALEIVQQLKVISGQYPLVFPGRNDPRKTMSEASMNQVFKRIGYTGKVTGHGFRH
                     TMSTILHEEGFNTAWIETQLAHVDKNAIRGTYNHALYLEGRREMMQWYADCIGRIGND
                     VN"
     gene            8329..9606
                     /locus_tag="PN977_001251"
     CDS             8329..9606
                     /locus_tag="PN977_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004009008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDI6041306.1"
                     /translation="MRYKPNQYLICDSYGNYYLRITLPVYMQPFFEGKRTFVRSLHTS
                     NLRVARRKRDQIADEYHCLRESVAPVNSTIENTLELLRSKAKYAKTATRVQDTASSCP
                     SLLKILEIYLTINSTKKKPATLAKARKAVEMFLSYRKKPDIALQDVSRTTVTGWIEHM
                     QKTLSQQSIANYISPMAQLWELASSRYHDAPERALSPWRGHRLDVAQSRESYEAFSNK
                     ELLQVLQVFSGNSAENKEMTALCLIGLYTGMRINEIASLTIDDVKEIEGVLCFEITQG
                     KTKAAARVVPVHNLITPLVLSLREKPHNGFLFYHASITERADGKRSTWHTQRFTRAKR
                     KALGEKGTERKVFHSLRHGVAQLLDRNQIPEDRIALLLGHTRGNTETFRTYSKNAASP
                     IELKKYIELLRYPEIEKGLSINKKSNLRRKTTL"
     gene            complement(9687..9971)
                     /locus_tag="PN977_001252"
     CDS             complement(9687..9971)
                     /locus_tag="PN977_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000378354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6041307.1"
                     /translation="MDSRQPFLLVTELKNYDNVVLVGMSYEEEASESLLFTLDFEQIR
                     LVSKATTSAIAVKTAPNKSVGGQVKMQVNTTEQKNQKSPGQDVVTPVFKQ"
     gene            complement(10116..10366)
                     /locus_tag="PN977_001253"
                     /pseudo
     CDS             complement(10116..10366)
                     /locus_tag="PN977_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001387929.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(10414..12255)
                     /locus_tag="PN977_001254"
     CDS             complement(10414..12255)
                     /locus_tag="PN977_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000891724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6041308.1"
                     /translation="MLELNVSRLSNVVTFQVDRPSLEKAKAEVEKLRKQMAAVKDIEL
                     RVKTHKQSTSKAKKDVDDIAKKQAQADKANAKAQLAAQRVVAREQKALAARKEKADLK
                     LLDVGSSISALHRLSVAEQYKAIAQAREIALQYERGAISLARMNSQMKRLQQQQRKIN
                     GNRNAHAKAYAPVKGSGSIGGGAAAVMLGGLGAGATYMAMSKASEFVTNSFANAETLG
                     ELYSRAKLGGVDVNQMNNIEQWAYKNGVDSMMGDQGKRKYLDQMKDVRERATKSYDEA
                     EYVVDKKTGKGEWKGGDSGINTLMNEGFLTKKDLKDFADNPAGLVSKAVQGMVKKGYS
                     DAQIGSRLEDLADDLMLTSKYWTRSAKEVEQSAREQRESGRWVTQAQQESVIKFRELN
                     NALSGLSDSQGIAFVDGFMKSLDPKVIEEFKKSMVAMLPMFTKLGEAIGGLVNAVMKT
                     INWLMKNDEKTEAIQKNLGDAPPLSNEGMKQNLSNLTPDQYKGAGTGTTKPDNSNSLV
                     NTIKSWFMPEETVGGAQAVNQYSLEGQNIANLKQSAAQQAFKAPSYTFAPVINFNPEL
                     QVNAEVPLTIESDTGRLSEFIDFKSKASSAEFSKLLTLGVMSGGSTY"
     gene            complement(12290..12487)
                     /locus_tag="PN977_001255"
     CDS             complement(12290..12487)
                     /locus_tag="PN977_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001091092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6041309.1"
                     /translation="MNTENPQSFILKAVKELAAISEESVINTSALCRLLEIDANNVRQ
                     RVFQTGCSTFEAIQYYCSKKQ"
     gene            complement(12639..13670)
                     /locus_tag="PN977_001256"
     CDS             complement(12639..13670)
                     /locus_tag="PN977_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001613953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6041310.1"
                     /translation="MNDLSTYFGVNTAVVTPFAYENALNTLITNLDLFDVQYHTENSI
                     LLTDLIDQFVQVAGETSPYAQSDWNASKRQEWSNKLFQMKHVGSINAVTSRDLEQYSR
                     QYPNRSDAMLVAVNDFSEAHYRSHANRVEVDLITALLGLKVESEYAGQGDLDFLDGQA
                     KTTVPAIDFASTTVNPFLAIRKAVRLQSEKLGSGLAAKRTGVIIFAAGTAADGLSSNP
                     LISDMVKYAGDASATALFTRMVDSNPAYDSFQIGGITVIDVSMFPEIVAHIGENGFAI
                     VPVMDKAAQCYQLHSGVGVRHAELGQDAVLHHQYLIKDEFQFPSVVTETSYLPVNNIP
                     QAIIFGSAA"
     gene            complement(13685..14068)
                     /locus_tag="PN977_001257"
     CDS             complement(13685..14068)
                     /locus_tag="PN977_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000013185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6041311.1"
                     /translation="MANPYGKLKAVNADNSRSRCKVVAYAADPVLQDRLVKLASPLTD
                     DLIVGALLKADGTKATTASDIAHVVVEPAYEGQESVVVAHPTFVILAEDGIEFNSMEK
                     ASVIAKLQSLGFVIAGYEELAIPTT"
     gene            complement(14073..14270)
                     /locus_tag="PN977_001258"
     CDS             complement(14073..14270)
                     /locus_tag="PN977_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001532247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HDI6041312.1"
                     /translation="MKQEMIISKDGETMIVSDENLIKVAALLGVTPESLRSTTAQLFK
                     SADAVSLADALKSKIKQLKGK"
     gene            complement(15255..15563)
                     /locus_tag="PN977_001259"
     CDS             complement(15255..15563)
                     /locus_tag="PN977_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000390658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HDI6041313.1"
                     /translation="MEEDISNAMEQLLDLTISLQTRINQAYSDLCDYTLSQCVISLMK
                     VNVSLDDFISLGSDLELSEDDAKQCNQIITDIATTLKVASEEIQQLVDDLAKSAIFSK
                     "
     gene            complement(15563..15766)
                     /locus_tag="PN977_001260"
     CDS             complement(15563..15766)
                     /locus_tag="PN977_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004012243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATPase"
                     /protein_id="HDI6041314.1"
                     /translation="MIGYIQETQLFQMILSDAKNYAELIRLAKETEDYEALERYRDEV
                     LTLKTPFNIVLNMINDALQGEYA"
     gene            complement(15824..16207)
                     /locus_tag="PN977_001261"
     CDS             complement(15824..16207)
                     /locus_tag="PN977_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000532779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6041315.1"
                     /translation="MGRKKKNNLKRIEKNSNFNSKRQRPPTHTNNEIPGEEIIFILET
                     ESPELSDEELEIIQSMPGVYFRPRTRKQTIISDGTLKRWQLLGFSSYHHFLGFNAFNN
                     CVGGASEVDLMAHLEDTLASNKRSN"
     gene            complement(16305..16574)
                     /locus_tag="PN977_001262"
     CDS             complement(16305..16574)
                     /locus_tag="PN977_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001581993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6041316.1"
                     /translation="MTYNITIGNKTIEITESGYNILKAILDIEFSPPTVVSFCSLGGY
                     SAQHVNHWLNHFTSFGVLDYEGINSTTFRLLKLNKDFELFLTNNQ"
     gene            complement(16584..17153)
                     /locus_tag="PN977_001263"
     CDS             complement(16584..17153)
                     /locus_tag="PN977_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005766820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDI6041317.1"
                     /translation="MINEEQFVKVPMWILKISQVNGHKFTDSAKNLYCYLRGFDRAFP
                     SYAKIGDVFGITPRAAEERVKKLLEMGLIIKDARPGSSNLYRVLDIPKGESSLPDPSE
                     KEDESNEILNVSEIIVEEETQEPEFPERPEIFTWLLSLDKKKPPHEETEDFVKRALDS
                     KNIYLCENDFDSLIIDLKSESTIYDSLPF"
     gene            complement(17207..17377)
                     /locus_tag="PN977_001264"
     CDS             complement(17207..17377)
                     /locus_tag="PN977_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000005469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6041318.1"
                     /translation="MSETTEKKRQPRTCFHIPDEVFQVLFKEARSKRITVTDYILDML
                     KREAETLSNKSK"
     gene            complement(17399..17575)
                     /locus_tag="PN977_001265"
     CDS             complement(17399..17575)
                     /locus_tag="PN977_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000873435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6041319.1"
                     /translation="MKYFTPQDVVEAWKRGEINRFKVRMNRNTARRCGYPEREKCFDD
                     ALKIIDELRKAEKE"
     gene            19260..19811
                     /locus_tag="PN977_001266"
     CDS             19260..19811
                     /locus_tag="PN977_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001273868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDI6041320.1"
                     /translation="MANIGYIRVSTVQQNTDRQLAGVTLDKVFEDKASGKNTQRPQFE
                     AMMNYVREGDVLHVHSIDRLCRNTADLLATVEQLTERGVSVHFHKEDFKTGKNSPAGN
                     MMLTVLAAVAQMEREMMLERQREGIAAAKAAGRIAKRGNGKAIDRAGIVAALANGGSI
                     RSVAKDFSVSTQTVQRIKKEQEA"
     gene            20152..20310
                     /locus_tag="PN977_001267"
     CDS             20152..20310
                     /locus_tag="PN977_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000550449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6041321.1"
                     /translation="MHLLSKHLHTIHFLVIDVFVVAISYRICIASSEEMDAVAIIAQA
                     MAMLTKRL"
     gene            complement(20377..20529)
                     /locus_tag="PN977_001268"
     CDS             complement(20377..20529)
                     /locus_tag="PN977_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000788776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6041322.1"
                     /translation="MKNIAAIKRNNRKIHARKFLSTPEGKAWLERKQRENEERKLLSE
                     LKWLKD"
     gene            complement(21136..21211)
                     /locus_tag="PN977_001269"
     tRNA            complement(21136..21211)
                     /locus_tag="PN977_001269"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(21176..21178),aa:Thr,seq:cgt)
     gene            complement(21327..22580)
                     /gene="proA"
                     /locus_tag="PN977_001270"
     CDS             complement(21327..22580)
                     /gene="proA"
                     /locus_tag="PN977_001270"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HDI6041323.1"
                     /translation="MLEQMGIAAKQASYKLAQLSSREKNRVLEKIADELEAQSEIILN
                     ANAQDVADARANGLSEAMLDRLALTPARLKGIADDVRQVCNLADPVGQVIDGGVLDSG
                     LRLERRRVPLGVIGVIYEARPNVTVDVASLCLKTGNAVILRGGKETCRTNAATVAVIQ
                     DALKSCDLPAGAVQAIDNPDRALVSEMLRMDKYIDMLIPRGGAGLHKLCREQSTIPVI
                     TGGIGVCHIYVDESAEIAEALKVIVNAKTQRPSTCNTVETLLVNKNIADSFLPALSKQ
                     MAESGVTLHADAAALAQLQAGPAKVVAVKAEEYDDEFLSLDLNVKIVSDLDDAIAHIR
                     EHGTQHSDAILTRDMRNAQRFVNEVDSSAVYVNASTRFTDGGQFGLGAEVAVSTQKLH
                     ARGPMGLEALTTYKWIGIGDYTIRA"
     gene            complement(22592..23695)
                     /gene="proB"
                     /locus_tag="PN977_001271"
     CDS             complement(22592..23695)
                     /gene="proB"
                     /locus_tag="PN977_001271"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HDI6041324.1"
                     /translation="MSDSQTLVVKLGTSVLTGGSRRLNRAHIVELVRQCAQLHAAGHR
                     IVIVTSGAIAAGREHLGYPELPATIASKQLLAAVGQSRLIQLWEQLFSIYGIHVGQML
                     LTRADMEDRERFLNARDTLRALLDNNIVPVINENDAVATAEIKVGDNDNLSALAAILA
                     GADKLLLLTDQKGLYTADPRSNPQAELIKDVYGIDDALRAIAGDSVSGLGTGGMSTKL
                     QAADVACRAGIDTIIAAGSKPGVIGDVMEGISVGTLFHAQATPLENRKRWIFGAPPAG
                     EITVDEGATAAILERGSSLLPKGIKSVTGNFSRGEVIRICNLEGRDIAHGVSRYNSDA
                     LRRIAGHHSQEIDAILGYEYGPVAVHRDDMITR"
     gene            23983..25038
                     /gene="phoE"
                     /locus_tag="PN977_001272"
     CDS             23983..25038
                     /gene="phoE"
                     /locus_tag="PN977_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoporin PhoE"
                     /protein_id="HDI6041325.1"
                     /translation="MKKSTLALVVMGIVASASVQAAEIYNKDGNKLDIYGKVKAMHYM
                     SDNDSKDGDQSYIRFGFKGETQINDQLTGYGRWEAEFAGNKAESDTAQQKTRLAFAGL
                     KYKDLGSFDYGRNLGALYDVEAWTDMFPEFGGDSSAQTDNFMTKRASGLATYRNTDFF
                     GVIDGLNLTLQYQGKNENRDVKKQNGDGFGTSLTYDFGGSDFAISGAYTNSDRTNEQN
                     LQSRGTGKRAEAWATGLKYDANNIYLATFYSETRKMTPITGGFANKTQNFEAVAQYQF
                     DFGLRPSLGYVLSKGKDIEGIGDEDLVNYIDVGATYYFNKNMSAFVDYKINQLDSDNK
                     LNINNDDIVAVGMTYQF"
     gene            complement(25077..25478)
                     /gene="crl"
                     /locus_tag="PN977_001273"
     CDS             complement(25077..25478)
                     /gene="crl"
                     /locus_tag="PN977_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HDI6041326.1"
                     /translation="MTLPSGHPKSRLIKKFTALGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWKNVPVKDAEVVERLEHTLRE
                     FHEKLRELLTTLNLKLEPADDFRDEPVKLTA"
     gene            complement(25536..26780)
                     /gene="frsA"
                     /locus_tag="PN977_001274"
     CDS             complement(25536..26780)
                     /gene="frsA"
                     /locus_tag="PN977_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HDI6041327.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     TQAMVWQQKACAEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDEA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITDWIEKRLC"
     gene            complement(26872..27330)
                     /gene="gpt"
                     /locus_tag="PN977_001275"
     CDS             complement(26872..27330)
                     /gene="gpt"
                     /locus_tag="PN977_001275"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HDI6041328.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            27591..29048
                     /gene="pepD"
                     /locus_tag="PN977_001276"
     CDS             27591..29048
                     /gene="pepD"
                     /locus_tag="PN977_001276"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HDI6041329.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRKPATAGMENRKPVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVAADKVDAL
                     KSLVNTYQEILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIESVGHYWTLLTELLKEIPAK"
     gene            complement(29105..29719)
                     /locus_tag="PN977_001277"
     CDS             complement(29105..29719)
                     /locus_tag="PN977_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor H"
                     /protein_id="HDI6041330.1"
                     /translation="MILLQLSSAQGPEECCLAVKKALDRLIKEATRQDVAITVLETEA
                     GRYPDTMRSALISLDGDNAWALSESWCGTIQWICPSPYRPHHGRKNWFLGIGRFTADE
                     QEQSDAIRYETLRSSGPGGQHVNKTDSAVRATHLASGISVKVQSERSQHANKRLARLL
                     IAWKLEQQQQENSAALKSQRRMFHHQIERGNPRRTFTGMAFIEG"
     gene            complement(29716..30855)
                     /locus_tag="PN977_001278"
     CDS             complement(29716..30855)
                     /locus_tag="PN977_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000521550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
                     /protein_id="HDI6041331.1"
                     /translation="MGKYIRPLSDAVLTIASDDLWIESSAIQQLHTTANLPDMQRVVG
                     MPDLHPGRGYPIGAAFFSVGRFYPALVGNDIGCGMALWQTDIFARKYNADKFEKRLSA
                     LDDVAEESWLEENLPSALAQHPWRSSLGSIGGGNHFAELQQVDEIIDAELFALTGLDA
                     QHLQLLVHSGSRGLGQSILQRHIASFSHHGLPEGSDDALSYIVEHDDALAFARINRQL
                     IALRIMQQIKANGNSVLDVAHNFVSACRIGDQQGWLHRKGATPDDNGLVIIPGSRGDY
                     SWLVQPVRSEETLHSLAHGAGRKWGRTECKGRLAAKYTATQLSRTELGSRVICRDKQL
                     IFEEAPQAYKSAESVVQCLVQAGLIIPVARLRPVLTLKNSGGKKG"
     gene            complement(31046..31498)
                     /locus_tag="PN977_001279"
     CDS             complement(31046..31498)
                     /locus_tag="PN977_001279"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDI6041332.1"
                     /translation="MNNIQIRNYQPGDFQQLCAIFLRAVTMTASQHYSPQQIAAWAQI
                     DESRWKEKLAKSQVRVAVINAQPVGFITRIGHYIDMLFVEPEYTRRGVASALLKPLIK
                     SESELTVDASITAKPFFERYGFQTVKQQRVECRGAWFTNFYMRYKPQH"
     gene            complement(31508..31907)
                     /gene="yafO"
                     /locus_tag="PN977_001280"
                     /pseudo
     CDS             complement(31508..31907)
                     /gene="yafO"
                     /locus_tag="PN977_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706233.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YafO"
     gene            complement(31910..32203)
                     /gene="yafN"
                     /locus_tag="PN977_001281"
     CDS             complement(31910..32203)
                     /gene="yafN"
                     /locus_tag="PN977_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system antitoxin YafN"
                     /protein_id="HDI6041333.1"
                     /translation="MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSAS
                     AFEALMDMLAEQEEKKLVKARFRPSAARLEEITRRAEQYLNDMTDDDFNDFKE"
     gene            complement(32255..33310)
                     /gene="dinB"
                     /locus_tag="PN977_001282"
     CDS             complement(32255..33310)
                     /gene="dinB"
                     /locus_tag="PN977_001282"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HDI6041334.1"
                     /translation="MRKIIHVDMDCFFAAVEMRDNPALRDIPIAIGGSRERRGVISTA
                     NYPARKFGVRSAMPTGMALKLCPHLTLLPGRFDAYKEASNHIREIFSRYTSRIEPLSL
                     DEAYLDVTDSVHCHGSATLIAKEIRQTIFNELQLTASAGVAPVKFLAKIASDMNKPNG
                     QFVITPAEVPAFLQTLPLAKIPGVGKVSAAKLEAMGLRTCGDVQKCDLVTLLKRFGKF
                     GRILWERSQGIDERDVNSERLRKSVGVERTMAEDIHHWSECEAIIERLYPELERRLAK
                     VKPDLLIARQGVKLKFDDFQQTTQEHVWPRLNKADLIATARKTWDERRGGRGVRLVGL
                     HVTLLDPQMERQLVLGL"
     gene            complement(33381..34304)
                     /gene="lafU"
                     /locus_tag="PN977_001283"
     CDS             complement(33381..34304)
                     /gene="lafU"
                     /locus_tag="PN977_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001521902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative lateral flagellar export/assembly
                     protein LafU"
                     /protein_id="HDI6041335.1"
                     /translation="MRKTGNRRERGAKTTIVRRQIKKNHAGHHGGAWKVAFADFTLAM
                     MALFMTLWIVNSVSKSERESIIAALHGQSIFNGGGLSPLNKISPSHPSKPATVAVPEE
                     TEKKARDVNEKTALLKKKSATELGELATSINTIARDAHMEANLEMEIVPQGLRVLIKD
                     DQNRNMFERGSAQIMPFFKTLLVELAPVFDSLDNKIIITGHTDAMAYKNNIYNNWNLS
                     GDRALSARRVLEEAGMPEDKVMQVSAMADQMLLDAKNPQSAGNRRIEIMVLTKSASDT
                     LYQYFGQHGDKVVQPLVQKLDKQQVLSQRTR"
     gene            complement(34307..35170)
                     /gene="motA"
                     /locus_tag="PN977_001284"
     CDS             complement(34307..35170)
                     /gene="motA"
                     /locus_tag="PN977_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001169515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor stator protein MotA"
                     /protein_id="HDI6041336.1"
                     /translation="MQKILGLLVVLGCVFGGYFEAGGQLVAIWQPAEMIIILGAGFGA
                     MIIGNPKHVLKEIAHQIKGVISKKQLGPEFQRQLLMCLYELLEMVQNGGLRMLDQHIE
                     QPEESTVFQKYPLVLTQKRLVTFIADNFRLMAMGKIDAHELEGILDQELDTAEESLLT
                     PSRSLQRTAEAMPGFGICAAVLGIIITMQSIDGSIALIGLKVAAALVGTFLGVFICYC
                     LMDPLANAMEQQARAEHSLLECVRTVLVAQAGGKPTLLAVDAGRKLLHLASKPTFANL
                     DAWVNAMLEQE"
     gene            complement(35183..35902)
                     /locus_tag="PN977_001285"
     CDS             complement(35183..35902)
                     /locus_tag="PN977_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000938732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliA/WhiG family RNA polymerase sigma factor"
                     /protein_id="HDI6041337.1"
                     /translation="MLQDVLFESEEFAAAPVLTPQQESHYLQAYLPLVRKVVRQLAPQ
                     CNCIIDRQDMEQTALMGLLNAIRRYGLPDEGFAGYAVHRIRGAILDELRALDWRPRQL
                     RQKYHQMKDLIRETRKKLGHEPEWSELAVEGISHEDYLEYQQLEGAETLASLDELLNG
                     EGPGIPLAGRELEDQFVTQQMLQHALSQLSEKEQLILSMYYQHEMNLKEIALVLGLTE
                     ARICQLNKQIAQKIRDFVYPN"
     gene            complement(35922..36389)
                     /locus_tag="PN977_001286"
     CDS             complement(35922..36389)
                     /locus_tag="PN977_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001521900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated FliL family
                     protein"
                     /protein_id="HDI6041338.1"
                     /translation="MKKIVVAGVVSAVLALVVGAGAGWGVWHHYAGKGKPTAVARTEA
                     VETLDESKSVFVTLPETIVTLHDNNGADHYLSAELVMVVASDKEAEKIKHQEPLYQSI
                     AVECLTEMKFEDLRGMKISAIRKLISDALKKDLQRRKMSAPYKDLLVKKVVFQ"
     gene            complement(36397..37458)
                     /locus_tag="PN977_001287"
     CDS             complement(36397..37458)
                     /locus_tag="PN977_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001067462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HDI6041339.1"
                     /translation="MNPALLATLGTLAETASLKADILPPVSSENAPAFTLPKMAVAAV
                     AERVHSAKTSQQQATRPQENDPVAMQALMALLLPQPAAPHQDTPQPRNVATSPVIQQL
                     TKAVVQNAAQRPTQQQELTPLPPQLQELISQLPQAKPEQQARLATYASEDLHAIAPTQ
                     PRVSTQPARPKPELTRVNARPQVERKTEKVPDSEPVIARAVLQVKTPDLVSEHQEIVA
                     KPATLSMDELGEKLTTLLKDQIHFQLNKQQQISTIRLDPPSLGKLEIAVQLDNGKLMV
                     HIGANQSEVCRALQQFSDDLRQHLTAQNFMEVNVQVSSEGQSQQQQQSGHQQEEVSAA
                     LQLDDAPQFQQNESVLIKV"
     gene            complement(37455..37766)
                     /locus_tag="PN977_001288"
     CDS             complement(37455..37766)
                     /locus_tag="PN977_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000414995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FliT"
                     /protein_id="HDI6041340.1"
                     /translation="MEKQRRQLFALVEAMNEALDKQRWRRLPALHQQLMRQFHDYVAA
                     ETDAAQLCAVKAQLYGAFNALIKRREQRAEVLKARMEQHQRHQEGVLAYSIVNLLSEK
                     S"
     gene            complement(37771..38163)
                     /gene="fliS"
                     /locus_tag="PN977_001289"
     CDS             complement(37771..38163)
                     /gene="fliS"
                     /locus_tag="PN977_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000285320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HDI6041341.1"
                     /translation="MYQVQDGYSQYKEVDLAARTAAASPLELVLVLFSGLMDELERAR
                     SHIEGRRFEKKAQSINKCIDILNALTSSLEFETGGELVVNLSRLYDHCVYRLYEASGE
                     LSAEKIDEVMLILSNLREGWEGLSGKLG"
     gene            complement(38186..39502)
                     /gene="fliD"
                     /locus_tag="PN977_001290"
     CDS             complement(38186..39502)
                     /gene="fliD"
                     /locus_tag="PN977_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HDI6041342.1"
                     /translation="MINPRTIAQEIAYADVATQAANLQEKQSELDAESSGLDSLSSAL
                     SDFQSAVDALNSDTDGPVTFAATSNNDSATVSANSQAQAGSYSFFVEQLAQGQQTTFS
                     MGDDAFSATGTFELTMGGSTMDIDLSAADQNGDGDGFIDASELVNAINDSDDNPGVSA
                     ALVKTDGTTTIMLTSDSTGAQSAFSVSVTGHDASNDSASAPVATDVSSAQDAIIHLGS
                     ATGPEITNSSNTFDDVIPGVTMTFTEVSDSDSDLTTFNISEDSSASQEKVQTFVDAYN
                     TLIDTVDSLTTHGDDSTSAGVFAGDAGLSSLANQLDDIAHASYNGVSIVDYGITLDSH
                     GHLQIDSDQFNDAMKSDPDGLTSIFVGDNSMVAQMDSLIDTYTDSSNGIITLRQQNID
                     DQMSKIQDEGDQLTDTYNANYDRYLEEYTNTLVEVYTMKASMAAFA"
     gene            complement(39708..40622)
                     /gene="lafA"
                     /locus_tag="PN977_001291"
     CDS             complement(39708..40622)
                     /gene="lafA"
                     /locus_tag="PN977_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001570969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lateral flagellin LafA"
                     /protein_id="HDI6041343.1"
                     /translation="MLSINTNNASMAAVNAISKSSSSLSTSMERLATGNRINSSADDA
                     AGKQIANRLTAQSSGMGVALSNINDATAMLQTADSMFDEMSDVLGRMKDLSTQAANGT
                     YSDDDLQAMQDEYDELGQQMSDMLQNTTYGGTNLFGVSGTSNTGTDGLFQSAVTFQVG
                     AESSDTMTVNISSQLNTLVTDLSAISNSFSADQADTTGTAGVSGGTELTASGSANQMI
                     TSISTAMDDVSQIQSKLGASINRLNDTANNLTSMQDNTEVAIGNIMDTDYATEASNMT
                     KQQVLMQTGITMLKQSNSMSSMVSSLLQ"
     gene            41109..41951
                     /locus_tag="PN977_001292"
     CDS             41109..41951
                     /locus_tag="PN977_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HDI6041344.1"
                     /translation="MSIIINNWRMDPSLNALIHCETGETRRLGEYHFILLETLAKNAD
                     VVLSRSYLCAEVWKNRIVGGNSLPTAIHALRVAIDDDGKQQNIIKTIPKKGYLCNKEY
                     VSLPESSPAEALIITDQIQDTAPEEISSTTPPVPVRKKHKGLMGLALTAAVIFIGSTV
                     GYSHLKSTPDAPQLVKESINSPRIKIFHLSSGKENNSVPLLSQTLEPGKDKLDNLLSA
                     HNMTMTTYYKYVRNRLESDIVLRNQCNGSWQLTFNVEGWQNSDINSAMYQNLEKLLNT
                     VQKC"
     gene            complement(42010..42987)
                     /locus_tag="PN977_001293"
     CDS             complement(42010..42987)
                     /locus_tag="PN977_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001195949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein"
                     /protein_id="HDI6041345.1"
                     /translation="MQVGLTSSSLATGGAHSAAVSSSTVAPTQAVRQKLPATASEYPA
                     SPLITTRPQRYSVQLNDQLTTLQQADHYLGQLEQQLLDYRHSQRKGGQAQSSALMQML
                     DKRTALSGGAVDRQLQPVLQGEARVTFHSPDLTNLVHNPTPGTRMFSVSDGRQTQLSA
                     VMLSEDDNAAQYQTRLTNALRRVGVQVHQQADGISFSTTEKQWPDIERSLSVRTDGDK
                     SAFMPLKTFAEPSQADRLAQSLQQGGAGISQMLENINQQRAQMAVQQEKARQLIDGMS
                     RFPQTENAVQASENLGGVLDSANHNYQVLLQAVNGQARISSQTVRNLLG"
     gene            complement(43004..43933)
                     /gene="flgL"
                     /locus_tag="PN977_001294"
     CDS             complement(43004..43933)
                     /gene="flgL"
                     /locus_tag="PN977_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020219239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgL"
                     /protein_id="HDI6041346.1"
                     /translation="MRVTTQQTYVSMTQSFNNLSGSLAHVVEQMATGKEILQPSDDPI
                     AATRITQLNRQQSAIEQYQSNIDSASAGLSQQESILDGVNNSLLAVRDDLLEAANGTN
                     TADSLASLGQDIQSLTESMVAALNYQDEDGHYVFGGTINDQPPIVAVDDDGDGVTDSY
                     SYQGNSDHRQTTVSNGVEVDTNVAASDFFGSNLDVLNTLNSLSQELQNPDVDPADPQV
                     QSDLQNAVDVVDTASDDLNASIASLGETQNTMSMLSDAQTDISTSNDELIGSLQDLDY
                     GPASITFTGLEVAMEATLKTYSKVSELNLFSVL"
     gene            complement(43948..45324)
                     /gene="flgK"
                     /locus_tag="PN977_001295"
     CDS             complement(43948..45324)
                     /gene="flgK"
                     /locus_tag="PN977_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000367241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HDI6041347.1"
                     /translation="MDMINIGYSGASTAQVELNVTAQNTANAMTTGYTRQVAEISTIG
                     ASGGSPNSAGNGVQVDSIRRVSNQYQVNQVWYAASDYGYYSTQQGYLTQLEAVLSDDN
                     SSLSGGFDNFFAALNEATTSPDDSALREQVISEAGALSLRIDNTLDYIDSQSTEIISQ
                     QQAMVSQINTLTSGIASYNQQIAQAEANGDNASALYDARDQMVEELSGMMDVQVNIDD
                     QGNYNVTLKNGQPLVSGQQSSTIALETNADGTPTMSLTFAGTTSTMTTDTGGSLGALF
                     DYQNDVLTPLTDTINSMALQFADAVNNQLAQGYDLNGNPGEPLFIYDASNADGPLTVN
                     PDITADELAFSSSPDESGNSDNLQALINISTEPLEIANLGSVTVGQACSSIISNIGIY
                     SQQNQTEVDAASNVYSEAQNQQSSVSGVSMDEEAVNLITYQQIYEANLKVISAGAEIF
                     DSVLEMCS"
BASE COUNT        12160 a        10669 c         9973 g        12663 t
ORIGIN      
        1 agagccaata tatttatagc tatgtgggtg gctcatcgct gacggcatgt atgcgtaaca
       61 gagcatctgt atcatccgtt tgacgttttc gtcggcgatc ctgccagcta ttcggacatc
      121 ttgtgaaacc caaaggcagc ggctgacgaa gcttatccgg tatcctctcg caacaaaggt
      181 acatgtacca tctacttttt gggcggccat tgtcgacaga ttcttaagta gaacagctaa
      241 gtttagattt aggaaaaagt tgttaaaatc aagcaaatac ttatcatcat cacttgattt
      301 cgtaaacgca aaactacgat actattaaca aattcttcta tgcattctta ccgaatgttg
      361 gcggtagcgt gcttaatcac tcggatttag tatgaaaaaa aattctttaa atgtgatttt
      421 ggatactgtt ttctcggcga aaagcatcaa tggtgagaaa tctcattctt ttatatcgta
      481 taaattagtt aaagctgtag aaaatttaag tggatatgac ttggatcttt tcctcaatcg
      541 tgcgataaac catcctaact tccctagcaa tatggatttt tccttacgaa cagtttttac
      601 aattgaacaa tctaacacta ttgaatttaa aaatttcatt tcagaattgg tgtggtacaa
      661 gcatattttc atacgatata aaaaacacct taatgatata ttatcggcaa aggcaagact
      721 tgagaagtta gttcttttcg caacaggtag tgaatgtata aaatatttag atgaaataga
      781 aagctcttat ggagttagtt tttggagcat tgatgctcgt ctcttaataa ataaagtttt
      841 gcttaacgaa agcaatgggt tatatgttaa atcaattcta tctaaaaccc actataaact
      901 tacagagttt atgttacagc agctattatt taagcatcaa gtacataact ttgatgattt
      961 ttcaaagaac cttataaaaa tactagatga tatgcgcacc actcaagata atgggtatgc
     1021 tagaaattta gctgatggta tttcatcctt tttaatacct ttagagtttg ataaaaatat
     1081 agatctcaaa aatagaacct tagcaccatt taccaacctt cctttaatag atcaatttat
     1141 catattccag agaactataa gcgatttaca acttcatgga aatggtttaa gaaatagcga
     1201 gttaagctta attgcagaaa ttaacagctt tattaaaaat aactatctca acaatatttt
     1261 agatggaaga gagcgtaata tagagaatat tgatgaacac tataaaagtg ttattcgacg
     1321 ttatactata ggtgattata ctgggtgtat ttcagaaata aattcagttc gaacgcagaa
     1381 gacaattttg ccattaattg aaatatatgc aaaatcccat gtgtatcttg ataagaaaat
     1441 ttcagactca tgcatattca ataaattaac aaactggctt atagcaatta taaaatgcga
     1501 cagacgcgct ggaaaataca tcgatgaatt tgaactttta gcatctaaga tatatttcaa
     1561 ttcaaattca tcctctctat tcttcacatt atataattta ataagtgata accataataa
     1621 attaaacata tctcgtataa atctaaccaa aaatggttcc tttgtaacat cccttcattt
     1681 gaatataaca atcaaggaac tatggactga acttggaata aaagagaatg agataccaaa
     1741 atatcgatta tttaaattca aagacctagc aaatctatcc tttgatgaaa tcaaaaaaca
     1801 ctacgaagta tacaatgata atgtaataat tcgctctgaa tatttaaaag attatactaa
     1861 ctttttatta gaaaatgaaa agtatgaatt atgcatccaa tttatagctg aaaactgcat
     1921 cggcaacccc gcaaattatt actacttccc aatccgaaaa ataattgatt ttgtaaaaga
     1981 caatattttt gactatgcaa gcgtggcctt atctatattg atagatattt ttgtcaaaag
     2041 cacatcaaac acttcaaatg agcttcttct cgaaagttat gagagtttag tagatatttc
     2101 aaatattaat aggccatcta cccattattg ctctaagcaa ttaactccat tagagcacta
     2161 tttccttaag tttatctgta taccttccgt gatggattca gacccttcat tcacaggcac
     2221 ggacgacttg aagaaggaaa gaattgctat tatagatcta ttattaaaag aacacgatga
     2281 tgcagattta ttgaaagaga aagacgaaat tatagatgaa atatcattcg aagaaataaa
     2341 aacaaaatat gagacaggaa aaatatttgt tgatatagaa aatttaaaac gggcaaagct
     2401 tgaaagatac agatattatt ttgatgcact taaggattcg cttattctcg gactagaacc
     2461 acctgaagac tttgctttaa ttacagacga tgggaatatc acagccattc catctggcga
     2521 tacaaactct ataatccatg aacttctaaa agaactaatc tccgattttg ttaaaaatga
     2581 aaattacggt cttgataaat atttgagtgc agatatacgg catggtgttt ttgaaaatca
     2641 actcagatca agtgctgaaa aatcgcaatt aataactgat atggatggta ctggacagta
     2701 ttcgaaaagc aatttaatta ttgagacata tccattaatt aaccctatta taaataacga
     2761 aatagggaca gcaatagctg atttctctta tcactttgac attgaattgg ctaaagccaa
     2821 ttcctggttc aatgtcaaga caatgctcat aagtgaatca cagcaaggta tgattgattt
     2881 cctcatttct gttgatatgt ttaattcatt taaagcagca gtttcaaatc aagcatcttt
     2941 tgagaagttt tttgacgctt gtattaactt catgtgggaa agaacgttca gatgtttgaa
     3001 tgatattaaa gagcgattgc attacgaatt taagaggaat attttagatt taattgccac
     3061 tcttaggcat caaattgata tctatagaag acgtagttcc atgagagaaa tccaggagaa
     3121 aattgacctc ttggccgaaa gcattaataa agaaattgca acagtctgcg attggttaaa
     3181 tgtacttgag tataacgagg agaaaatata taaaatatca tccgtgatgc aggcatgtag
     3241 gaaaactttt tttaatatac accattgttc tgatgatgct atcgtttttg attcaatgta
     3301 tcatgatgat gaaccaaaat tatcttataa agaagccaag cctctaatca catcaataat
     3361 aacagcgtta aacaatgcta tggcctatgg taataaaaaa atctttatca gtattaagcc
     3421 tgaagaaaag tcatggaaaa taaccatcag aaatttgata attgagacaa agaacagaac
     3481 taccaaccaa attttacaag agattgatga taaaataaaa cgaggggata atagtctaaa
     3541 tataaaagaa ggcggagctg gaatatataa aatatatgat ttattatgtt cattaccgca
     3601 aagatttaat gtgaatcatt gcatccaaaa taacgaattc attttaaata ttgagataaa
     3661 gaaatgaaaa ttcttcttgt tgaggatatt gaatataagc gtgataaggt gatcggttta
     3721 ctagagtcta tttctgccga tgtatcagtc gatgtagcta aatcttatgt ttcggcagta
     3781 aattccgcaa ccagtaaaac ttatgatctt ataatattag atatgagcct tcccacttac
     3841 gataaggggc ctaatgaaaa tggaggtcgc ttcagagtgt acggagggaa agatattatt
     3901 cgcaagttaa tgagaggaaa agagcatcca gtagttgtgg ttttaacaca acatacaact
     3961 tttggtgaaa ttggtgagca aaaaaatact gacactctat cacaagagtt gaatgcttta
     4021 atgggatcaa aatttatagg tattgtaaaa tatgactcaa ctaaacctca atggaaagat
     4081 gaaataaaaa aagtcgtaca ggatttgtta tgattaaaat attaatagtt gatgacaata
     4141 aatctagaat tgaaaagcta aaatctagct taactgaact catcacaaaa aacatgataa
     4201 ggattgatga gaaatataca agtgacgccg caaaaatcgc gctcaagtta aatcaatatg
     4261 attatttaat tcttgatgtc tttttaccta aaaaagataa ctatagccca gatgaaagaa
     4321 atggattagg tcttttaaaa caaataaatt cagattctaa attttactct ccaaaaaaaa
     4381 tagttggcat aactgcatat ctgaatgata tatccagata tgaatccgaa ttcagagaat
     4441 atgcttcaat tatattcgag gctcgacgta atgatactgg atggcttgaa tcactcaaaa
     4501 aaatcattga aaaggatgtt gaatctcaag taagttataa tctcaatgaa aaagatagtg
     4561 ttttaataac agtacacggt ataagaacct acgcaccttg gcaaaatacc atagaagaaa
     4621 aaataaccaa tatatcaaat aaatttaact atataaaatt caattatggt tttctaaata
     4681 ttctttgttt tttattccct ccgactagac atctatttgc aagaaaaata atccaagaca
     4741 tacgaattac agtggaatca aataagaata aaagaattta tattatttgt catagttttg
     4801 gtacctacct tgtatattgt gccttaagta aactgacaca tacggatgca aaaattgaat
     4861 gtttaatttt ttctggtagc gtattaaaac gtactacctc attgaaaaca ttaaagcaac
     4921 attgtaatgc aataataaat gactgtgctg taagtgatta tattttatta ctatgtaaaa
     4981 tgttagtcat tggcttgggc gatgcaggca gaaaagggtt tattgagcca aatgatggtg
     5041 ttttcattaa ccgctatttt aaaggaggac attcaactta cttcgaagat aaagatttca
     5101 tagaagtgaa ctggcttcct ttaatttttg acaataaaaa tatcgcgtca cgagatgaaa
     5161 gaaaaaatca tattttttcc gatgtcacca atgctttgca aaatataatt gaatatttaa
     5221 aaataccaat atggttattt ttttcttttt tattgatagc actggtatta taatatcact
     5281 gcacattaaa aatcatattg gagcttccct cataaagctc caatataaaa caccatcatt
     5341 tcaatagtac ctgcagctaa ttaaaactag tcaaccgaaa acattaatca ctttaaactt
     5401 tagtatgcac ttatactttc ttaataatag aaaaacactt acaaataaat taatcatgtc
     5461 aatctccgta actacaacct cggaaagtat gtaaacagga atcattactg aaaaaaacac
     5521 gatttcagca gaattctaga aatatcctgt gctgctgccg ataccaactt tcgtatatac
     5581 tacccttcac gttccttctc gtttttataa aactattcat ttataactat caataggcat
     5641 cataaccaat gtaagtgtaa gtactggtga acgagtgtca atggagttaa aagtatcact
     5701 tcatcctgct aaatataagt tatcctacgt tttaatatac tagaactcaa aattagaatg
     5761 gtttgttaat attaatagat ttaccattga tatcttttga caccttaaaa aaatctatac
     5821 cttttatcca ctgacataac tgttgtagtg tttccattgc ctgttctaac tcattttttt
     5881 caattctaac gcccccatga tggctacagg aatttaataa ttcctgagct agagtgatgt
     5941 cattcttcag cttattaggc agaccgagaa aattaatcac cctcgacaaa ctcccaagca
     6001 ttaacttttg atagtcttct tcaaattcct tttcagccct aatatttact aacatcaaaa
     6061 catactcaag ataacgccgt gctgtatttc ccttttcttg taacgcatct ctatcatact
     6121 ctttaatatc actcaattcc tgtagtagaa aatcaaactt agctattgcc aagttaagat
     6181 tgatgttatg cattagcaat tcggatgcat gattgctaac aggtacacga tttggtggac
     6241 gtatcattat agccgtcgtg taacaattta tatttggtat ctgtataaat tcctcaccgg
     6301 aacgcgtaac acgttgaact tctaccatat agtggccaaa tttatcaatc tcatatgacc
     6361 gacaacgagc agaatataat ttatttagct ttttcctgtc ttgataatat gattttatta
     6421 atacaaaaaa tgctctttcg tgctcaataa ttaaaatagt ctctattgaa gcatggtaca
     6481 accatccgtt attccatgtt aattcaagct gtctatttcc atctttaaaa tcaattttag
     6541 ttatacgatc ccaacttcct ctatcaataa aatcgacaat attgcgccca aatccttgat
     6601 agttattttt aacaatgtgc aatagtactg cctttatttt atcccaatca tgaatttctt
     6661 tttcatattc accatttaat atattcgtta aatcactgtg aaaattttca tatgtcataa
     6721 gtatctcttt ttagataaaa ttggatttcg acaagtagac gttgataaca acaagactgg
     6781 ttagataatt taatcattga tgcaacgttt agcgttgaat caattgacat cattaccaat
     6841 tcttccaatg caatcagcat accactgcat catctccctc cgcccttcca gatacaaagc
     6901 atggttgtac gtcccacgaa tcgcattctt atcgacatgc gcaagctggg tttcaatcca
     6961 tgccgtattg aacccttcct cgtgcaaaat cgtactcatc gtgtgacgga aaccatgccc
     7021 cgttaccttc cccgtatacc caatccgttt gaatacctga ttcatactcg cttcactcat
     7081 cgtcttgcgg ggatcatttc gccctgggaa taccagtgga tattgcccag atatcacctt
     7141 gagttgttgt acgatttcca gcgcttgggt agataggggg acaaggtgag gccgtttcat
     7201 cttcatacgc tctgcaggta tttcccacac tgctttttca agatcaaact cactccagaa
     7261 agcacctcgt agctcgccag tacgaactcc tgtaaggatc agcagacgag cggcaagaac
     7321 aactaacggg cttcctgtgt agcctgcgag agctttaaag aagtctggta actcctcaac
     7381 agtaaggaag ggataatgct tcgattcatg ccctgacatt gcgctggtta gatccgctgc
     7441 aggattgtat tccgcacgac cggtaacgat ggcgtaacga aagacttcac tgcaacgctg
     7501 gcgaaccttc ttggccttct ctgtcgcgcc acggctttcc attcgacgta gcacattcag
     7561 cagaaccaaa ggcttgatgt cattcaccgg cagttggcca atataaggga aaatatcttt
     7621 attgaaggct tcgataatgt cggaggcata accttctgac caccggctca ccttcgttcc
     7681 gtgccattca agtgccacag cctgaaacgt attattgagc tgcacatcgc gaaccaactt
     7741 ttcttctttc ttggcaaacg atgggtcgat accttcggcc agctttttct tggcttcatc
     7801 acgcagtgcc ctcgcttgtg caagggacac tgccggatag acaccaaaag ccatacgttt
     7861 ctcttttcca ttgaagcgat atttcatccg ccagtatcga gaaccagaag gaacaacttc
     7921 aagatacaag cctgcaccat ctgctagctt gtaggctttc tctctgggtt tagcagcatc
     7981 tacctgtcga gcattaagct tcattggggg catctccctg gaccgaacac agaatgcccc
     8041 cacttatgcc cccaactgcg acttgatttc ggttgagtcc agttgataac aggagataag
     8101 atacgggcca gaaaccgcag tatacgggct tttagttgat ttcggtagac ttgggaagag
     8161 tttgaaatgg tgccgataat aggagtaaaa cactcgatta atctcgtgtt aatgccatca
     8221 cctacgacca ttactaattg attgaatctt atagaaaaat catcattttc tatgcgtcac
     8281 aatcaatttt cacattacaa acgctccgac actttagccg gagattttat gagatacaag
     8341 cctaaccaat acttaatctg tgattcttac ggtaactact atttaaggat cacgctgcct
     8401 gtgtatatgc agcccttttt tgaaggaaaa aggacgtttg tcagaagtct acacacaagt
     8461 aatcttcgtg ttgcacgtag aaagcgtgat cagattgcag atgaatacca ttgcttacgg
     8521 gagagtgttg cccctgtaaa cagcacaata gaaaacacgc tggaactgtt acgcagtaag
     8581 gctaaatacg ccaaaacagc taccagagtg caagatacag cgtcttcgtg tccgtcattg
     8641 cttaaaattc ttgaaatcta cctgacaatt aacagcacga agaagaagcc agccacttta
     8701 gctaaggcaa gaaaagcggt agagatgttt ctctcctacc gtaaaaagcc tgatattgca
     8761 ttgcaagatg taagccgcac cactgttaca ggctggattg aacacatgca aaaaactctt
     8821 tcacaacaat caattgcaaa ttatatcagt ccaatggccc agctatggga attagcttca
     8881 tcacgttacc acgatgcgcc agaaagggcg ctctccccct ggcgagggca taggcttgat
     8941 gtggcacaaa gtagagagag ctacgaggca ttttctaaca aagagctatt gcaggtgttg
     9001 caagtatttt ccggtaattc agcagaaaac aaagaaatga cggctttgtg tcttatcggt
     9061 ttatatacag gtatgcggat caatgagata gcaagtctca caatagacga tgtgaaagag
     9121 atcgaaggtg tgctgtgttt tgaaatcaca cagggaaaga cgaaagctgc ggcacgtgtt
     9181 gtgcctgtgc ataaccttat cactccgttg gtgttgtcgc tgcgtgaaaa gcctcacaat
     9241 ggctttttgt tctatcacgc cagcattaca gaacgtgctg acggtaaacg ctctacgtgg
     9301 catacacaac gatttacaag agctaaacga aaggctttag gggaaaaggg aacagaaagg
     9361 aaagtgtttc attctctgag acacggagta gcacagcttc ttgatcgaaa tcaaattcca
     9421 gaagacagga tcgcccttct cctgggccat acacgcggca atacagaaac attccgcaca
     9481 tatagcaaaa atgcagcttc tccaatagag cttaaaaaat atattgagct tttacgctac
     9541 cctgaaatag agaaaggctt atcaatcaat aaaaaatcaa atttaaggcg taaaacaacg
     9601 ctatagacgt aataaagagg cttcaatata ggctgatgcc tcttttatga ttatctcgtt
     9661 acagagcgtt tcataggcgt ttaaggctat tgtttaaata ctggcgttac aacgtcttgc
     9721 cctgggcttt tttgattttt ctgttctgtt gtatttacct gcattttaac ctggcctcct
     9781 acagatttat ttggtgctgt ttttaccgca attgcgcttg tagttgcttt agagacaaga
     9841 cggatttgct caaaatcaag agtaaacaat aggctttcgc ttgcttcttc ttcgtaagac
     9901 atgccaacta acacaacgtt gtcataattt tttaactcag tgacaagtag gaaaggttgg
     9961 cgactgtcca tcaactgatt taggtattca agcataagca tacgtcgatt gccgttaatt
    10021 ccagcattct taatcatatc aggattgagc ttcaatacgg tttcagagat acgcccttgt
    10081 aatgtgaact tgttattccg tatttgaaca tgatcactaa cttctgatcc tgattcgacg
    10141 gcatagcttg taacgtccgc atttctgcta actttcgtcg attccacgct atcaaaaatg
    10201 ataatgcctg gtagtcattg taagcagcat ttgcaccact gccgagatta gaggtaatga
    10261 tcgcaaagcc gttaccccct ttggttgttc taccgttgct atcgttgctt gtgctgttga
    10321 ttttcgcctc agtcgggcga ccaatggtta acggacccat tgccataatt tgtaatcctt
    10381 atttaagggg gctaaacagc cccacagtga tgattaatag gttgacccgc ctgacataac
    10441 acccagcgtt aatagcttgc taaactcagc ggaggatgcc tttgatttga agtcgataaa
    10501 ttcgctcaat cgtccggtgt ctgattcgat agttagcggc acttctgcat ttacttgtaa
    10561 ttctggattg aagttaataa caggtgcaaa ggtgtaagaa ggagccttga aagcttgttg
    10621 tgctgcactc tgtttcagat ttgcaatgtt ttgtccttcg agactgtatt gatttacagc
    10681 ctgcgcccca cctaccgttt cttctggcat aaaccagctt ttaatagtgt taaccaggga
    10741 attgctgtta tctggcttag ttgttccggt gccagcgcct ttatattgat caggagtgag
    10801 attagaaaga ttctgcttca ttccttcgtt agatagtggc ggtgcatccc ctaaattctt
    10861 ctgaatagct tccgtcttct cgtcgttttt catcagccag ttgattgttt tcattacggc
    10921 gttaaccaat ccgccgatag cctcgcctaa tttggtaaac attggcagca ttgcaaccat
    10981 gctcttttta aactcttcaa tcactttcgg atcaagagat ttcataaagc catcaacaaa
    11041 ggcgatccct tgtgagtcag aaagcccact aagtgcatta ttcagttcac ggaatttaat
    11101 aacgctttcc tgttgtgcct gtgttaccca gcgccctgat tctcgttgtt ctcgtgctga
    11161 ttgttccact tctttagcgg agcgtgtcca gtatttagag gtgagcatca aatcatcagc
    11221 cagatcttcc agacgactac cgatttgtgc atctgaatag cctttcttca ccattccctg
    11281 aactgcttta ctaaccagac cagccggatt atcagcaaag tcttttagat cctttttagt
    11341 taagaatcct tcattcatta aagtattgat gccgctatcg ccgcccttcc attcaccttt
    11401 gcctgttttt ttatcgacta catattcagc ctcatcatag cttttggttg cacgctcacg
    11461 cacatctttc atctgatcga gatacttacg tttaccttga tcccccatca ttgaatcaac
    11521 gccgtttttg taggcccatt gctcaatatt gttcatctgg ttaacgtcta caccgcccag
    11581 cttcgcacgg ctatacaatt cacccagcgt ttcagcattc gcaaagctgt tagtaacaaa
    11641 ttcagaggct ttgctcatag ccatatacgt agcgccagcg cctaagccac ccagcataac
    11701 agccgcagca ccgccgccaa tactcccgct acccttaaca ggtgcatagg ctttagcgtg
    11761 agcgttacga ttgccattaa tcttacgttg ttgctgttgt aggcgtttca tttggctatt
    11821 catacgtgcc agactaatag ctcctctttc atattggagg gcaatttctc tagcctgtgc
    11881 aatagcttta tattgttccg ctacagaaag gcggtgcaat gcactaatgc ttgatccaac
    11941 gtctaacagt tttaagtctg ccttctcttt gcgtgctgcc agggcttttt gttctcttgc
    12001 aactacacgt tgagcggcta attgtgcttt agcattggct ttatctgcct gtgcttgctt
    12061 tttagcaata tcgtctacgt ctttttttgc tttagaagtt gattgtttat gtgtcttaac
    12121 acgcaattct atatctttca ccgccgccat ttgtttacga agtttttcta cttctgcttt
    12181 tgctttctct aatgatggtc gatcaacctg gaaggtgaca acgtttgata agcggctgac
    12241 gttaagttct aacatgctaa ttgtttccct gtattatgat tgttgtttat tattgttttt
    12301 tagagcaata gtattgaatt gcttcgaaag tgctgcaccc agtctgaaag acacgctggc
    12361 gcacattatt tgcatcaatt tctaaaaggc ggcaaagcgc cgatgtgttt ataactgatt
    12421 cctcgctaat agctgccagt tcttttacag ccttaagaat aaaggattgt gggttttctg
    12481 tattcattgg ttttctctca catatatatt tttatttggg tttgtagaca gtagaaaaac
    12541 aaaaagggca aagagattcg ttactgtatt tgcgcctaca ggttagttaa tccctttacc
    12601 cttattgccc cgatggggaa tcgtctatta acgctttatt acgcagcaga tccgaagatg
    12661 atagcttgcg gaatgttgtt tactggcaga taagatgtct cagtaaccac agatgggaat
    12721 tggaattcat ccttgattag atattggtga tgcaacacgg catcttgacc taactcagca
    12781 tgacgcacac caacgccgct atgaagctgg taacactgtg ccgctttatc cattaccgga
    12841 acaattgcga aaccgttctc tccgatgtga gcaacaatct caggaaacat tgacacatca
    12901 ataacagtga tcccaccaat ctggaaagag tcataggctg ggttagaatc aaccatacgg
    12961 gtaaacaggg ccgtagcact cgcatcgcct gcatatttca ccatgtcact gatcagcggg
    13021 ttagatgaca aaccatcagc agcagtgcca gcagcaaaga taattacacc agttcgcttt
    13081 gcagcaagac cagaccccag cttttcactc tgtaagcgca ccgctttacg aatagcaagg
    13141 aacgggttaa cagtagtcga tgcaaagtcg atagcgggta ccgtggtttt agcctgaccg
    13201 tcgaggaagt cgagatcacc ttgtccggca tattcagatt caactttcag gcccagcagc
    13261 gcggtaatca gatccacttc aacacggttt gcgtgtgaac ggtaatgagc ctcagagaaa
    13321 tcattaaccg ctaccagcat agcatcagaa cggtttggat attggcggct gtattgttcc
    13381 aggtcacgag aagtaacagc gttaatactg ccgacgtgtt tcatctggaa aagtttgttt
    13441 gaccattcct gacgtttaga ggcgttccag tcgctttgag cgtaaggcga ggtttcccca
    13501 gctacctgca caaactgatc aatcaggtcg gtaagaagaa tgctgttttc tgtatgatat
    13561 tgcacatcga acagatcgag attagtaatc agggtattaa gtgcattctc ataagcaaat
    13621 ggagttacta ccgcagtatt aacgccaaaa tatgttgata aatcattcat tattattttc
    13681 cttgttaagt cgtagggatc gcaagttctt cataaccagc aattacaaag ccgagagatt
    13741 gcagttttgc aataacactt gccttttcca tgctgttaaa ttcaatccca tcttcagcca
    13801 gaataacgaa agtaggatgt gcaaccacta cgctttcctg tccttcataa gccggttcaa
    13861 ctactacgtg agcaatatca gatgcggttg ttgctttggt gccatcggct ttcagcaaag
    13921 ccccgacaat aagatcgtca gtgagaggtg aagccagttt gacgaggcga tcttgcagca
    13981 ctggatcagc agcgtaagct actaccttac agcgtgaacg ggagttatcc gcattcactg
    14041 cttttaattt tccatatggg ttagccataa gattattttc ctttcagttg tttaatttta
    14101 gatttaaggg catcagcaag actaaccgca tcagccgatt taaataattg agcagtggta
    14161 gaacgaagac tttcgggagt aactcccaac agtgcggcaa ccttaatgag gttttcatca
    14221 ctaacaatca tcgtttcccc gtctttgctg ataatcattt cttgtttcat aaataatgtt
    14281 cctgttagtg ccagacaaat aaaaagcccc aatgccgtaa gaggttaatt ctgaccagca
    14341 aaagggcttt aacgtgcggg caattacata attgataagt taaactataa gaggattaaa
    14401 catatcagca aatacacttt ttaaatatac ttgatgatat gcttaaaggg tgaatagata
    14461 ctcctgaaaa catgctctta ggaaaacatg cggaatcaat caggaatatc tattcggggc
    14521 gcggtggcca accgctatct ttcatggtgt ggatgcacca gtcagatttt aaggaatcac
    14581 tattccaatc tactcacaga ttaaattttt aataatgatt aactacacca acagtaatct
    14641 accgtttcag aaaatcgttt ttacaaccaa agcttataga tccgcattct ataaaacccg
    14701 ttttgctatc aatatttcca aaatttattg caccattctt agctaaactc tctctgcttg
    14761 cggctaacgc tatgcagatg attgtttttg acaacaactt aatagattat tctgttacta
    14821 acttctgtca ttaaataata gtttaatgat gttagtaaat agttataaaa tataactatc
    14881 agtgtaaggc tctttaatgt tttctgacaa aataaggagc tttaggataa gcatagatga
    14941 aaaataagag cgggaagggc aaggagataa cacctacccg cttttatcaa aattaagcac
    15001 aatgcctaac aacattcatc aacacaacgt agaaacaatc aacaatcaaa gagggagtta
    15061 tgattattct aaaacttctt gagagatgga aggtaatatt tgttagaggt taatgccact
    15121 caacctttga ggctatcacc ttccatatgc agggatttat gacagtagaa ggattaacac
    15181 caactaaaat ctttttatct acccctaaaa ttattatata acaaaatcat tataaatgca
    15241 aataattttc attatcattt agagaaaata gcactttttg ctaaatcatc tactaattgc
    15301 tgaatctctt cacttgcaac ctttaacgtg gtggcgatgt ctgtgatgat ttgattacac
    15361 tgttttgcat cgtcctctga tagctctaaa tcgctcccaa gagagataaa atcgtcaagg
    15421 cttacattca ccttcattaa gctaataacg cattgagaga gcgtgtaatc gcataaatct
    15481 gaataggctt gatttattct tgtttgtagt gagatagtca gatctaaaag ttgttccatt
    15541 gcattgctaa tatcttcttc cattatgcgt attctccttg taatgcatca ttaatcatat
    15601 tcaatacgat gttaaaaggt gtcttgagcg tcagcacttc atcacggtaa cgctctaatg
    15661 cttcgtagtc ttctgtttct tttgccagcc gaattaactc tgcatagttt tttgcatcag
    15721 acaggatcat ctgaaataat tgtgtttctt gaatatagcc aatcattatt tttccttttt
    15781 tgaatatttt cattgggcgc ttatacatac acccatcatt ttattagtta gaacgtttgt
    15841 tagaagctaa tgtatcttct aaatgagcca tcagatcgac ttccgatgca ccacctacac
    15901 aattattgaa agcgttaaat cccaaaaaat gatgatagga gctaaaccct aaaagctgcc
    15961 aacgtttcag agtgccatca gaaatgatag tttgttttct tgttcttggg cgaaagtaaa
    16021 cgcctggcat tgattggatt atttctaatt cttcatcact taattcaggg gattctgttt
    16081 caagaataaa gattatctct tcccctggaa tttcgttatt ggtgtgtgtc ggtggacgtt
    16141 gtctttttga attgaaatta ctgttttttt caatacgttt taagttgttc tttttctttc
    16201 ttcccatttt tccctctttt tatgtggatt ataaaaactg gacgccattg ttacgatgaa
    16261 tatcgccata aatgcatttg ccatatgtat tgcccgatct gttgttattg attgtttgtg
    16321 agaaacaatt caaaatcttt gtttaatttc aaaagacgaa atgtagtgct gttaatccct
    16381 tcataatcca gcacaccgaa cgatgtaaaa tgatttaacc aatgatttac gtgttgtgcg
    16441 ctgtagccgc ctaaactaca aaagctaacc actgtcggtg gtgaaaactc tatgtccaaa
    16501 atagctttta aaatgttgta tccgctttca gttatttcaa tagttttgtt tcctatagtg
    16561 atgttataag tcatttattc tccttagaag ggtagtgaat catatattgt gctctcactt
    16621 ttgagatcaa ttatcaaaga gtcaaaatca ttttcacaaa gatagatatt ctttgaatct
    16681 aatgctcgtt ttacaaaatc ttctgtttct tcgtgcggtg gtttcttctt atcaagggaa
    16741 agaagccaag taaaaatttc gggccgctca gggaactctg gctcttgtgt ttcttcttca
    16801 acgatgattt cggaaacatt caatatttca ttagattcgt cttctttctc agaaggatca
    16861 ggtagtgatg attctccttt tggaatatca agcactcgat aaaggttaga gcttcctggt
    16921 cgtgcatctt ttataattaa tcccatttcg agcaatttct taaccctttc ttctgctgct
    16981 cttggtgtta tgccaaaaac atcgcctatc ttagcgtaag agggaaatgc acgatcaaac
    17041 cctctcagat agcagtaaag gtttttagca ctatctgtaa atttatgtcc gtttacttgg
    17101 cttattttta gaatccacat aggaactttt acaaattgtt cttcattaat cattaaaaat
    17161 ctccttgatg taatcacgct ctacggcgct actatggtga taaaaatcac ttgcttttgt
    17221 ttgagagtgt ttcagcctct cttttcaaca tatcaagaat gtaatctgta acagtgatac
    17281 gtttacttct ggcttcttta aacaacacct gaaatacttc atcaggaata tgaaagcaag
    17341 tgcgtggttg tcttttcttt tctgtagttt cgctcatagt tttctccttc tcctgttatt
    17401 attctttctc tgccttacgc agttcatcaa taatttttag tgcatcgtca aagcattttt
    17461 cacgctctgg ataaccacag cgtcgcgctg tgttcctgtt catccttact ttaaaacgat
    17521 taatctcacc acgtttccag gcttcaacta catcctgtgg agtaaaatat ttcattaatt
    17581 agcctcctta ttctatttgg ttaaaagaaa aaacaccttg caagccttgt tgggcttcaa
    17641 agtgcggtgc attggtttaa ttacttagag gataagaatg ttttctgtcc ctaatattac
    17701 tttaccacga ctaaaagctt ttgtcaaccc ctagccataa aaatattgat cattttcagt
    17761 gtgttataat ttttcttagc aattcgtgct cttaagagtg ctcctattct tccttattct
    17821 ctaaaacgct ctaaaacgcc ctctgacgag gttttgataa atacaataca ttcccttatg
    17881 tagaggctgt ttagctcgcc agaggcttat ttaaggggct tattttgtat ttgttaggta
    17941 tatgaaaagg agatgataga taaactctaa tttcagacac cttataaaat acctgtaaag
    18001 ctcctcagaa gctctataac gagataatct attaccgcta agtgtttccc tgccttatat
    18061 acgtttagct cgcctacggc ttgatttaag cggtagaatc tcatctacta cctaacatct
    18121 ttaaaccata tttttctttt ttgtctgttt gatggttgat ctatagttat gcgttagcat
    18181 ccccaaaggg gtaacgctac tctaattgca ttcgccagaa cattgcacaa gaaatttatt
    18241 ttagatgatt gatgatcgtt agattggcgt aagcctcatc aatggaacta ttgaagcgca
    18301 cagcgcatag actatttagc cttacgcaag ccgtaggcta tttgcacata gtagattaca
    18361 tattcgttgt gagcggcatt agcgaacata gatctactaa ctaacatcaa caaacaaaaa
    18421 ctatgtgtta tgacaagcag tctaacgact aacactctaa cttgtgcaag gttctggcga
    18481 atgccaatag attaagggat agtcgaagac tccctaacgg gtatcactta attattcttt
    18541 aaagataaaa gaatttataa aagataattt aattgtagtt tgatagttga tctaataggt
    18601 aacaaaattc tgtaggggtg gctaacagtt ttctgtgggg gtatctaaca taattctgtt
    18661 agggttgggg taacagtttt ctgttagggt gcatatttct ttaaaagtta aggataacag
    18721 aattctgtgg gggtagttca cagtgaccta ccgttacatc catttatcac ataacatctg
    18781 tgcaatgatc ttctaaaagt gtttcttgct accaaaatta aacatctaaa acaggcttaa
    18841 attcaatgta attgatgatt aatccctgtg taattcctac gtaaaaatcc aatgaaaagt
    18901 agcttaaaaa gtgcaatgtt agctttcttc acttttacat cactttcgca aagccttgtg
    18961 taatacggct tacagagcac tatcagaaat tgttgattaa aaatcaatct attatttgtc
    19021 gaacaagttt cctttaatgc aacaaaaaat tattttttca aaataaacac actatttaat
    19081 agcttgctaa ttttaccaaa atgtaactac atgattctca tgcaagacgt gtagtgataa
    19141 agcaggctaa atcaccacac taaaaacctt cgaatttcgt agtgaaaaaa gtcctttaca
    19201 cacctaatca acacgatcaa taatcactac acacaaatca acacagaagg aaaatgaaaa
    19261 tggctaatat tggatacatt agggtatcga ctgtgcaaca aaacacagac cgtcaattag
    19321 ctggagtaac tcttgataaa gtctttgaag ataaagcgtc aggcaagaac actcagcgcc
    19381 cacagtttga ggcaatgatg aactatgtgc gggagggtga cgtgttacac gtccatagta
    19441 ttgatcgcct ttgtcgtaat acagccgacc ttctggcaac ggttgagcaa ttaacagagc
    19501 gcggcgtgtc tgtgcatttc cataaagaag actttaagac agggaagaac tctcccgcag
    19561 ggaatatgat gctcacagtg cttgcagcag tggcacaaat ggaacgtgaa atgatgcttg
    19621 agcggcagcg tgaggggatc gccgcagcta aggccgctgg acgtatagca aaacgtggta
    19681 atggaaaagc aattgatcga gcgggaattg ttgccgctct cgcgaatggt ggatcaattc
    19741 ggagcgtagc aaaagatttc agtgtcagca cacagacggt gcaacgtatc aagaaagagc
    19801 aggaagcgta atgcctcctg atatttgaga attgtcgctg cgtggacctg acgccgctga
    19861 tcttcgcgtt aaacattgat cacgtctgcg ccgatttcag agaaaaagac agtaactgaa
    19921 ctcactatga aggctaaacg tatctttaca cttgatgaaa acgttcaaaa aacaacctaa
    19981 ccccaaaatt caacaaattg caacacattg tttttaaaag aaaattaaat ctacatcaaa
    20041 tattggttga cgtagattga ggtgaatagt agactattag ggtaaattac cacccttgta
    20101 acatcattta atgcatgttc taatcttcct atcgtaaaac cggaggaagt tatgcattta
    20161 ctttcaaaac atcttcacac tattcacttt ctcgttattg atgtattcgt tgtggctatt
    20221 agttaccgca tttgcatcgc ttccagtgaa gagatggacg ctgttgcaat tattgctcaa
    20281 gcaatggcga tgttaactaa acgactttaa taatttcact atcaggcgca taaacacatc
    20341 tttgatcgct ggatgtgtta tgcgcctact ggtgattcaa tccttaagcc atttcaactc
    20401 actaaggagt tttctttctt cgttctctct ctgtttacgc tctagccagg cttttccttc
    20461 tggcgtagac aggaacttac gagcgtgaat cttgcggtta ttacgtttta tagctgcaat
    20521 gtttttcatc gtgttgcctc tgtattcata tgaaaattaa caagttacat actacacagg
    20581 gagactacgc ataaaacaca atacaagtaa agatacattt tcaagacaat aggagccata
    20641 aagcgccttt gatggttata ccaaaaaata agcgccgtag gcgtatccac taaaaagggc
    20701 tttttcacaa aagctatgaa gtcttagcag ttcttctctt atcaggcacc cgttgtactc
    20761 cggtggacga aaccgtgcta gagcttgatt ctttcccgat caagttaggt tttgaagagg
    20821 tgggctttaa tccactattg cacttaaaac tcttcccatc atttagcctg tttatcctct
    20881 gcaacgtctc acgacgtgta gcatcattag acaccgcgcc aggtgtggaa catcttgcta
    20941 agagttttgg tagaaggttt agagacggtg gcacgtcttc cagcttcaaa ggaacttgtt
    21001 acgaggttga gcctgggaac tactcgccca tacgtgttac actaccaaag cccataacaa
    21061 cgtataggcg tttgtaagtg ataacagaag atacaatttc ttagagaagt taagctaagt
    21121 tattgatttt cttgatggtg ccgataatag gagtcgaacc tacgaccttc gcattacgaa
    21181 tgcgctgctc taccaactga gctatatcgg ccctgaaagg acatgattca cgaacgtgaa
    21241 tcacggtgga caaggttaaa actaaccggg cgatgcgtca atggccttgt gaatcaaatg
    21301 gctacttttg catcacccgg ttttatttac gcacgaatgg tgtaatcacc aatgccgatc
    21361 cacttgtaag tggtcagtgc ttccaggccc atcgggccac gcgcgtggag tttttgtgtg
    21421 cttaccgcca cttccgctcc cagaccaaac tggccgccat cggtaaaacg cgtagaggcg
    21481 ttaacgtaaa cagcggatga atctacttca ttaacaaaac gctgggcgtt gcgcatatcg
    21541 cgggtcagga tcgcatcgga gtgttgcgtg ccgtgttcac gaatatgggc gatagcatcg
    21601 tcaagatcgc tgacgatttt gacgttcaag tctaatgaca ggaactcatc gtcatactct
    21661 tcggctttaa cagccaccac cttcgcgggg cctgcctgca attgcgccag cgcagcagca
    21721 tctgcgtgta acgtcacgcc actttccgcc atttgcttgc ttaatgcggg caggaagcta
    21781 tctgcgatgt ttttatttac cagcaacgtt tctaccgtat tacacgtgct cgggcgctga
    21841 gttttcgcgt tgacaatcac tttcaatgct tcagcgatct ctgcactttc atcaacgtaa
    21901 atatggcata cgcctatacc acctgtgatt accggaatcg tcgactgctc gcggcacagt
    21961 ttatgcaacc cagccccgcc gcgtggaatc agcatgtcga tgtatttatc catacgcagc
    22021 atttcactga ccagcgcgcg gtcaggatta tcaatcgcct gtactgcgcc tgccggtaaa
    22081 tcgcaggatt tcagggcgtc ctgaatcacc gccaccgttg cagcgttagt gcgacaggtt
    22141 tctttgccac cacgcaggat cactgcgtta ccggttttca ggcacagcga agcgacatct
    22201 actgtcacgt tcgggcgagc ttcataaatc acgccaatca cccccagcgg tacgcgacga
    22261 cgctccagac gcagaccgct gtccagcaca ccaccatcaa tcacctgccc caccggatcg
    22321 gcgaggttgc acacctggcg cacatcatcg gcaatgcctt tcagccgtgc gggcgtcagt
    22381 gccagacggt caagcatcgc ttcgctaagg ccattggcac gcgcgtcagc aacatcctgg
    22441 gcgttagcgt tgaggatgat ttcgctttgt gcttccagtt catcggcgat tttttccagc
    22501 acgcgatttt tttcgcggct ggagagttgc gctaatttat aagaggcttg cttcgcggca
    22561 atgcccattt gttccagcat cagcctgctc cttaacgggt aatcatgtca tcacggtgaa
    22621 cggcaaccgg gccgtattca tatcccagta ttgcatcaat ttcttgcgag tggtgtccgg
    22681 caatacggcg taatgcatcg ctgttgtaac gactgacacc gtgggcgata tcgcgacctt
    22741 cgaggttgca aatgcggatg acttcaccac gcgagaaatt gccagtcacg cttttaatcc
    22801 ctttcggcaa cagggagctg ccgcgttcaa gaatggcggc agttgcccct tcatctaccg
    22861 tgatttcacc cgccggcggc gcaccgaaaa tccagcgttt acggttttca agcggagtcg
    22921 cctgggcatg gaacagcgta cccacggaaa tgccttccat cacatcacca ataacgcccg
    22981 gcttgctgcc cgcggcaata atggtgtcga tacccgcacg gcaagccacg tccgcggcct
    23041 gcaatttggt actcatgccg ccagttccga ggcctgaaac gctgtcaccg gcaatcgcgc
    23101 gcagtgcgtc atcaatgccg taaacatctt taatcagttc tgcctgcgga ttgctgcgcg
    23161 ggtcagcggt gtacaaacct ttttgatcgg tcagcagcaa cagtttatcg gcacccgcca
    23221 gaatcgccgc cagcgcagaa aggttatcgt tatcaccgac cttaatctct gcagtagcga
    23281 cagcatcgtt ctcattgatt accggaacga tattgttatc gagcaacgca cgcaaggtgt
    23341 cgcgggcgtt caggaagcgt tcacggtctt ccatatcagc acgggtcagc agcatttgcc
    23401 cgacgtgaat gccataaata gaaaacagct gttcccatag ttgaatcagt cgactctgcc
    23461 ctaccgccgc cagcagttgt ttcgaggcga tagtcgctgg cagttccggg taacccaggt
    23521 gctcacgtcc ggcggcgatc gcgcccgacg tcacaataac aatccgatgc ccggcggcat
    23581 gtaactgcgc gcactggcga acaagttcaa cgatatgggc acggttcaga cggcgcgatc
    23641 cgcctgttag cacactggtg ccgagtttta ccaccagcgt ctggctgtca ctcatgattc
    23701 tctgccattc aattttaggg aaaatgatat caaacgaacg ttttagcagg actgtcgccg
    23761 gttgccaacc atctgcaagc aaagcatggc gttttgttgc gcgggatcag caagcgtagc
    23821 ggcagttgtt tacgctttta ttacagattt aataaattac cacattttaa gaatatcatt
    23881 aatctgtaat atatctttaa caatctcagg ttaaaaactt tcctgttttc aacggggctc
    23941 tcccgctgaa tattcgcgcg ttaattaaaa tcaggaatga aaatgaaaaa gagcactctg
    24001 gcattagtgg tgatgggcat tgtggcatcg gcatccgtac aggccgcaga aatatataac
    24061 aaagacggta ataaactgga tatctatggc aaagttaaag ccatgcatta tatgagcgat
    24121 aatgacagta aagatggcga ccaaagttat atccgttttg gttttaaagg cgaaacacaa
    24181 attaacgatc aactgacggg ttatggtcgg tgggaagcag agtttgccgg taataaagcg
    24241 gagagtgata ctgcacagca aaaaacgcgt ctcgcttttg ccgggttgaa atataaagat
    24301 ttgggttctt tcgattatgg tcgtaacctg ggcgcgctgt atgacgtgga agcctggacc
    24361 gatatgttcc cggaatttgg tggcgactcc tccgcacaga ccgacaactt tatgaccaaa
    24421 cgtgccagcg gtctggcgac gtataggaac accgacttct tcggcgttat cgatggtctg
    24481 aacttaaccc tgcaatatca agggaaaaac gaaaaccgcg atgttaaaaa gcaaaacggc
    24541 gatggcttcg gcacatcatt gacatatgac tttggtggca gcgatttcgc catcagtgga
    24601 gcctatacca actctgatcg caccaacgag cagaacctgc aaagccgtgg cacaggcaag
    24661 cgtgcagaag catgggcaac aggtctgaaa tacgatgcca ataatattta tctggcaact
    24721 ttctattctg aaacacgcaa aatgacgcca ataactggcg gctttgccaa taagacacag
    24781 aactttgaag cggtcgccca ataccagttt gactttggtc tgcgtccgtc gctgggttat
    24841 gtcttatcga aagggaaaga tattgaaggc atcggtgatg aagatctggt caattatatc
    24901 gatgttggtg ctacgtatta tttcaacaaa aatatgtcgg catttgttga ttataaaata
    24961 aaccaactgg atagcgataa taagctgaac atcaataatg atgatattgt cgcggttggc
    25021 atgacctatc agttttaatg gatattgccg gatgtgatgc atccggcaca tttcactcac
    25081 gccgttaact tcaccggctc atcacgaaaa tcatccgccg gttccagctt cagattcagt
    25141 gtcgtcagca gttcacgcag cttctcatga aactcacgca gggtatgttc cagtcgctca
    25201 accacttcgg catcttttac cggaacgttc ttccagtcgc ctgctttatc gaacagacca
    25261 aactggtaac tgtaggtaaa acgggattcc tgcgcttcca gttccatcca ccagccccag
    25321 aactcacgca cttccggtgc cggtttcacg ttgacgcata cagccagaca atcgaaaaag
    25381 aatcgattat cttcgcactt accttcacga atatacgggc ctagtgcggt aaattttttg
    25441 atcaatctgc tcttcgggtg tccactcggt aacgtcattg caatctcctg ttgtgatgca
    25501 actgttttac caaattggta aaatttagca aatttttaac acaatcgttt ttcgatccag
    25561 tcggtgattt cctgaagacc tttgtcaaaa ttccgataca ccgggttaaa tgggatctct
    25621 aacaatttac cgtcagcaga tgatgaggtg attaagcgtg agtcctcttc cgggctgaac
    25681 ggatcgttct tccagtagcc tgataacatt ggcgttgggc agcgacgtcc cagcaatcct
    25741 tgcactttta atgaatagcg attcagctcc acgcgcaacg cttcgtcgga agcatcatgc
    25801 atccccaaac ggctcgccag aacgtcaaga tacatttccg gcacctgttg ctggcactta
    25861 aaatcactca acagggtatg aactaccgga ccaagacagg caaccgcttt cagacgcggc
    25921 gattcaaggt atgccagacg cacggcaacg ttagcgccga aacggaaacc aaaggccgcg
    25981 acgcgagtgt gatctaccca cggtacgtta ggcagcgcct ttaagacgtg ctgatgcaac
    26041 aggctggagt cctgggtgag cttccatttt gaagaaaagc ccaccgacgg catatcaata
    26101 gtcagcatcg caatgccgcg cggcgcaaaa taacgttcat acaggctgta atagtccgtc
    26161 tgcatcgcat ccagaccacc acacattaat actgtcggga acgggccatc acctttcggc
    26221 atatgcaaaa agccggtgat gggcgcaccg ccgggtacgg taaactccat ctgccgcatc
    26281 gtgcccggca gacgctgagc ggcctcttca taggcgcggt ttgacaaagc ctgcgcttgc
    26341 tcggccaggt catctccttt cagatgagga taggcagcaa tgttgtacaa cgtagccgca
    26401 tgcagccagt gtcgaccgct gagttgcgga tcttcttccg cacaggcttt ttgttgccac
    26461 accatcgcct gggtagccca ctcataaatc cagttgccgc cgcggtagcc aatcaccgta
    26521 tcgtataaat catcgtcggt acgttcggca tcgctcatca caatacgcgc ctggacgtcg
    26581 aggatttcac gtgggtcaat gccgcgccag atccacatca gacggttaat catgcgatac
    26641 cagtgaggga tggttttacc atcaagggcc gactgcacag gcggttgtgc gccgtgatta
    26701 aagcggcgga ctagcgtcga ggtttcagga tgtttaaagc ggggtttgaa cagggtttcg
    26761 ctcaggtttg cctgtgtcat ggatgcagcc tccagaatac ttactggaaa ctattgtaac
    26821 ccgcctgaag ttaaaaagaa caacgcccgg cagtgccagg cgttgaaaag attagcgacc
    26881 ggagattggc gggacgaata cgacgcccat atcccacggc tgttcaatcc aggtatcttg
    26941 cgggatatca acaacatagt catcaaccag cggacgacca gccggttttg cgaagatggt
    27001 gacaaagtgc gcttttggat acatttcacg aatcgcaacc gcagtaccac cggtatccac
    27061 caggtcatca ataacgatga agccttcgcc atcgccttct gcgcgtttca gcactttaag
    27121 ctcgcgctgg ttgtcgtgat cgtagctgga aatacaaacg gtatcgacat gacgaatacc
    27181 cagttcacgc gccagtaacg cacccggtac cagaccgcca cggcttacgg caataatgcc
    27241 tttccattgt tcagaaggca tcagtcggct tgcgagttta cgtgcatgga tctgcaacat
    27301 gtcccaggtg acgatgtatt tttcgctcat gtgaagtgtc ccagcctgtt tatctacggc
    27361 ttaaaaagtg ttcgagggga aaataggttg cgcgagatta tagagatctg gcgcactaaa
    27421 aaccagtatt tcacatgagt ccgcgtcttt ttacgcactg cctctccctg acgcgggata
    27481 aagtggtatt ctcaaacata tctcgcaagc ctgtcttgtg ttgacaacat tttctgctaa
    27541 ccctgtgacc tgcaatactg ttttgcgggt gatcgacaag gagacttaac gtgtctgaac
    27601 tgtctcaatt atctccacag ccgctgtggg atatttttgc caaaatctgt tctattcctc
    27661 acccgtccta tcatgaagag caactcgctg aatacattgt tggttgggca aaagagaaag
    27721 gtttccatgt cgaacgcgat caggtgggta atatcctgat tcgtaaacct gccaccgcag
    27781 gtatggaaaa tcgtaaaccg gtcgtcttgc aggcccacct cgatatggtg ccgcagaaaa
    27841 ataacgacac cgtgcatgac ttcacgaaag atcctatcca gccttatatt gatggcgaat
    27901 gggttaaagc gcgcggcacc acgctgggtg cggataacgg cattggtatg gcctctgcac
    27961 tggcggttct ggctgacgaa aacgtggttc acggcccgct ggaagtgctg ctgaccatga
    28021 ccgaagaagc cggtatggac ggtgcttttg gcttacagag caactggttg caggctgata
    28081 ttctgattaa caccgactcc gaagaagaag gtgaaatcta catgggttgt gcggggggta
    28141 tcgacttcac ctccaacctg catttagatc gtgaagcggt tccagctggt tttgaaacct
    28201 tcaagttaac cttaaaaggt ctgaaaggcg gtcactccgg cggtgaaatc cacgttggcc
    28261 tgggtaatgc caacaaactg ctggtgcgct tcctggcggg tcatgcggag gagctggacc
    28321 tgcgccttat cgatttcaac ggtggcacac tgcgtaacgc catcccgcgt gaagcctttg
    28381 cgacgattgc tgtcgcagct gataaagtcg acgccctgaa atctctggtg aatacctatc
    28441 aggagatcct gaaaaacgaa ctggcagaaa aagagaagaa tctggcattg ctgctggact
    28501 ctgttgcgaa cgataaagct gccctgattg cgaaatctcg cgataccttt attcgtctgc
    28561 tgaacgccac cccgaacggt gtgatccgca actccgacgt ggcaaaaggt gtggtcgaaa
    28621 cctccctgaa cgtcggtgtg gtgaccatga ctgacaataa cgtagaaatt cactgcctga
    28681 tccgttctct gatcgacagc ggtaaagact acgtggtgag catgctggat tcgctgggta
    28741 aactggctgg cgcgaaaacc gaagcgaaag gcgcatatcc tggctggcag ccggacgcta
    28801 attctccggt gatgcatctg gtacgtgaaa cctatcagcg tctgttcaac aagacgccga
    28861 acatccagat tatccacgcg ggcctggaat gtggtctgtt caaaaaaccg tatccggaaa
    28921 tggacatggt ttctatcggg ccaacaatca ccggtccaca ctctccggat gagcaagttc
    28981 acatcgaaag cgtaggccat tactggacac tgctgactga actgctgaaa gaaattccgg
    29041 cgaagtaatt atttgatttg ctgccggatg gcgtttaatc gccttccggc agtttcatcc
    29101 ttcattatcc ttcgataaaa gccatccctg tgaacgttcg tcgcgggttg ccacgttcaa
    29161 tctggtgatg gaacattcgc cgctgcgatt tcagcgccgc gctattttcc tgttgctgtt
    29221 gttccagctt ccaggcaatc agtaatcgcg ccagccgttt gttagcatgc tgactgcgct
    29281 ctgactgaac cttcacgcta ataccggatg ccagatgcgt ggcgcgtacc gccgagtcgg
    29341 ttttattgac atgttgaccg cccggccccg acgaacgcag cgtctcataa cggatcgcgt
    29401 cggattgttc ctgctcatca gcggtaaaac gcccaatgcc cagaaaccag tttttgcgcc
    29461 catgatgagg ccgatacgga ctcggacaaa tccactggat agtgccacac cagctttcgc
    29521 ttagagccca tgcgttatcg ccatccagag aaatcagcgc cgaacgcatt gtgtcaggat
    29581 agcgccccgc ttctgtttcc agcaccgtta tcgcgacgtc ttgtcgggta gcttctttaa
    29641 tcaacctgtc cagcgccttt ttcactgcca ggcaacattc ttccggcccc tgagctgagg
    29701 agagttgtag caagatcatc cttttttccc tccactgttt ttgagcgtta gcaccggacg
    29761 cagtcgcgcg acaggaataa ttaacccagc ctgcaccaga cattgcacca cgctttcagc
    29821 cgatttataa gcttgtggcg cttcttcaaa gatgagttgt ttatcgcgac aaattacccg
    29881 gctgccaagt tcagtacgtg agagctgcgt cgctgtgtat ttcgctgcca gacgcccttt
    29941 acactcggtg cgcccccatt tacgcccagc cccatgcgcc agcgaatgca atgtttcctc
    30001 actcctgacg ggctgaacca gccaggagta atcaccgcgt gaaccgggaa taatcaccag
    30061 accgttgtca tccggtgtgg cacctttacg atgcaaccag ccctgttgat caccgattcg
    30121 acacgcgcta acaaagttat gcgccacatc cagaaccgaa ttaccgttgg ccttaatctg
    30181 ttgcattatg cgcaaagcga tcagctggcg attaatacgc gcaaacgcca gcgcatcatc
    30241 atgttccaca atataactta gcgcgtcgtc actgccttca ggcaaaccat gatgcgaaaa
    30301 cgaggcaata tgccgctgta aaatagactg gcccaaaccc cgcgagccgc tatgaaccag
    30361 cagttgcaga tgctgcgcat ccagacctgt cagtgcaaac agttcagcgt cgataatttc
    30421 atcaacttgt tgcagttctg cgaagtggtt tccgccaccg atggaaccca gcgagctgcg
    30481 ccacggatgc tgtgctaacg ctgacggcag gttttcctcc agccagcttt cttcagcaac
    30541 gtcatccagc gcagataatc gcttttcaaa cttatcggcg ttgtatttgc gcgcgaaaat
    30601 atctgtttgc catagcgcca taccgcagcc gatatcattg ccgaccagtg ccgggtaaaa
    30661 acggcctacg gagaagaacg ctgcgccaat cggatagccg cgtccggggt gtaaatctgg
    30721 catccctact acgcgttgca tgtcgggtaa atttgccgtg gtgtgtaatt gttggatcgc
    30781 tgaactctcg atccacaggt catcagatgc gatggtaagt accgcatcag ataagggacg
    30841 aatatatttg cccatgtacc attccattta tgaataaaaa tcaggaaatg gcagacgaat
    30901 ccagggcaag aaaaaaagaa ttaatcctgg aaaggtctgc aaagagtcat tgataaagga
    30961 tttgtcatga tttacctcct tgcagtttgt ggaataagaa aacgcgcaca tactaggcgg
    31021 gcgttatttt ctttgcaagc tggatttaat gttgcggttt atatcgcata taaaaattag
    31081 taaaccacgc tccccggcat tcaacgcgct gctgcttaac tgtctgaaaa ccataacgtt
    31141 caaaaaaggg ttttgcggtt atgctggcgt ccaccgtaag ttcggattca gacttaatca
    31201 aaggttttaa caaagcgctg gcaaccccgc gtcgggtgta ttcaggttca acaaataaca
    31261 tatcgatata atgtccaatg cgggtaataa aaccaaccgg ttgtgcatta ataactgcaa
    31321 cccgcacttg tgatttcgcg agtttctcct tccagcgaga ttcgtcaatc tgcgcccagg
    31381 cggcaatttg ttgtggtgaa taatgctgac tggcggtcat cgtaaccgct ctaaggaaaa
    31441 tagcgcatag ttgctgaaaa tcgccaggct gatagtttct tatttgtatg ttattcataa
    31501 tataaattca aaaacgcatg cgaaacgctt ctgccatttt cccaatttta tgcatttggt
    31561 tgttatctcg agccagttta tgaggttcag gtttcagaat ggcaatgagc aaccatgctt
    31621 gctcatcaaa cgcaccctga caatatacca aatgcgcttc gtcattcgtt ctgctgaatt
    31681 ggcgcaactg tggcggaaat ggattattca catttgccag atgaatatga gcaactcgct
    31741 caaatttgat taatggccag gtaaacgagt cgtcgtagga gtgcatcgcg accaaatata
    31801 tctggcaaaa caccgtcacg cttataggaa ataaaatccg ccgttaacgc atcaagttcc
    31861 tctgctgtaa gttgcaggcg aataagtttt gttttgaata cccgcatcct tattccttaa
    31921 agtcattgaa atcatcatcc gtcatatcat taagatattg ttcagcgcgg cgtgtaattt
    31981 cctctaatct tgcggcactc ggacggaagc gcgccttaac cagttttttc tcctcttgtt
    32041 cagcaagcat gtccattaac gcttcaaatg cgctggcact taagagatat cctgcggggc
    32101 gattattaga aagaaccgca accggttgat caataaagta tttagctggg tttttacgta
    32161 actcagtgat attgaccgat ttttcagcga gaattcgatg catacagtga tcccctcata
    32221 ataatgcaca tcagaatata cataatagta tacatcataa tcccagcacc agttgtcttt
    32281 ccatttgagg gtcaagtaac gtcacatgca gccccaccag acgcacaccg cgcccgccgc
    32341 gacgttcatc ccaggtttta cgcgcggtgg cgattagatc agctttattc agccgcggcc
    32401 agacgtgctc ctgagtggtt tgctgaaaat catcaaactt taatttcacc ccctggcgag
    32461 caatcagtaa atcaggtttt acctttgcca gacggcgttc aagttcagga tacagccgct
    32521 cgataatcgc ttcacattca gaccagtgat gaatatcttc cgccatcgtg cgttccacgc
    32581 cgacggattt tcgcaaccgt tcactgttaa cgtcgcgctc gtcaatcccc tgactacgct
    32641 cccacaaaat gcggccaaat ttgccaaaac gtttgagcag agtcaccaga tcacactttt
    32701 gcacatcacc gcaggtccgc agccccatcg cttccagttt tgccgccgag actttgccga
    32761 cgccgggaat ttttgccagc ggtaaggttt gcagaaatgc cggaacttct gccggagtaa
    32821 tcacaaactg accgttgggt ttattcatgt cggaagcaat tttggcgaga aactttaccg
    32881 gcgcaacgcc cgcagatgcc gtcagttgca gctcgttaaa gatcgtctga cggatttcct
    32941 tggcgatgag ggtcgcagaa ccgtggcaat gcacactatc ggtgacgtcg agataggctt
    33001 catccagtga taacggttca atgcgcgagg tatagcgcga gaaaatttcg cggatatgat
    33061 ttgaggcttc tttgtaggcg tcaaagcgcc ccggaagcaa ggtgagatgt gggcataatt
    33121 tgagtgccat ccctgtcggc atagcgctgc gtacgccaaa tttacgcgcg ggataattgg
    33181 cggtgctgat caccccccga cgttcgcggc tgccgccaat agcaataggg atatcgcgca
    33241 gggcgggatt gtcgcgcatc tccaccgctg cgaaaaagca gtccatatcc acatgaatga
    33301 ttttacgcat tgctcacctc tcaacactgg taaagtatac agtgatttca gggtttgaga
    33361 aatgcgtaaa gattcagcat ttaacgcgtt cgctgtgaaa gcacctgctg cttatccagc
    33421 ttttgcacca gcggctgcac cactttatcc ccatgctgac caaagtattg atacagcgta
    33481 tcggacgcgc ttttagtcag caccataatc tcaatgcgcc ggttgcccgc gctttgcgga
    33541 tttttcgcgt ccagcagcat ctggtccgcc attgcgctca cctgcatcac tttatcttcc
    33601 ggcattcctg cctcttccag cacccgacga gccgaaagcg cgcggtcacc cgaaaggttc
    33661 cagttgttgt agatattgtt tttgtaggcc atcgcatcgg tatgcccggt aataataatt
    33721 ttgttatcga gcgagtcgaa cactggcgcg agctccacca gcagcgtttt aaagaacggc
    33781 ataatctgcg cgctgccacg ttcgaacata ttgcgattct ggtcgtcttt aatcagcacg
    33841 cgtaatccct gcgggacaat ctccatctcc agattcgctt ccatatgcgc atcacgggca
    33901 atggtgttga tactggttgc cagctcacca agctcggtgg cggatttctt cttcagcagc
    33961 gccgttttct cgttgacgtc gcgcgctttt ttctccgtct cttccggcac tgccacggtg
    34021 gcaggttttg acggatgaga cgggctgatt ttatttaacg gcgacagccc gccaccgtta
    34081 aaaatcgact gcccgtgtag cgcggcgata atgctttcac gctcggattt actgacgctg
    34141 ttgacaatcc acagcgtcat aaacagcgcc atcattgcca aagtaaaatc ggcgaacgcc
    34201 accttccacg ccccgccgtg gtgcccggca tgattctttt tgatctgccg ccggacgatg
    34261 gtggtttttg ccccacgctc gcggcggttg cccgtctttc tcatgcttat tcttgctcca
    34321 gcatcgcatt cacccacgcg tcgaggttgg cgaacgtcgg tttggacgcc agatgcagca
    34381 acttacgccc ggcgtccacc gccaacagcg tcggtttgcc gcccgcctgg gccaccagca
    34441 cggtacgcac acactccagc agcgaatgtt ccgcccgcgc ctgctgttcc atggcgttgg
    34501 cgagcgggtc catcaggcag tagcaaataa acacgcctaa aaatgtcccc accagcgctg
    34561 ccgccacttt caggccaatc agggcgatag aaccgtcaat tgactgcatg gtgatgatga
    34621 tgcccagcac cgccgcgcaa ataccaaatc ccggcatcgc ttcggcggtc cgttgcagcg
    34681 agcgtgacgg cgttaacagc gactcttcag cggtgtccag ctcctgatcg agaatgcctt
    34741 ccagttcatg ggcgtcgatt ttacccatcg ccatcaggcg aaagttatcg gcaataaaag
    34801 tcaccagccg cttctgggtt aacaccagcg ggtatttctg gaaaacggta ctctcttccg
    34861 gctgctcaat atgctgatcc aacatccgca gaccgccgtt ttgtaccatt tcaagcaact
    34921 catacaggca cattaatagc tggcgctgaa actccggccc gagctgtttt ttactgatca
    34981 cgcctttaat ttgatgggca atctctttca gcacatgttt cgggttccca ataatcatcg
    35041 ccccgaaacc cgcacccaaa ataatgatca tttccgccgg ttgccagata gccactaatt
    35101 gcccgcccgc ttcaaaataa ccgccaaata cgcagccgag caccaccagt aaacctaata
    35161 ttttttgcat gatttcttca ctttaattcg gatagacaaa atcacgaatt ttctgcgcga
    35221 tctgtttatt cagttgacaa attcgggcct cggttagccc taataccagc gcaatctctt
    35281 tgagattcat ctcatgctgg taatacatgg agaggatcag ttgctctttt tcgcttaatt
    35341 gtgaaagcgc gtgttgcaac atttgctggg tgacaaactg atcttccagt tcccggccag
    35401 caagcggaat gcctggccct tcgccgttca gcagttcgtc aaggctggcg agcgtttccg
    35461 ccccttccag ttgctgatat tcgagataat cttcatgcga gatcccttcc acggccagtt
    35521 ctgaccactc aggctcgtgc cccagttttt tacgcgtttc gcgaatcagg tctttcatct
    35581 gatgatattt ctgtcgcagc tggcgcggtc gccagtcgag agcgcgcagc tcatccagga
    35641 tcgcaccgcg aatacggtgc acggcatagc ccgcaaaacc ttcgtccggc agcccgtagc
    35701 ggcgaatggc attaagcagc cccattaacg ccgtttgttc catatcctga cgatcaataa
    35761 tgcaattaca ctgcggggca agttgccgca ccactttacg caccagtggt aaataggcct
    35821 gcaagtagtg gctctcctgc tgcggggtta ataccggcgc ggcagcaaat tcttctgact
    35881 cgaataatac gtcctgtagc atgtttatat ccgcctggtg attactggaa gaccactttt
    35941 ttcaccagca aatccttata cggtgcgctc attttgcgac gctgaagatc tttctttagc
    36001 gcatcagaaa tcagtttgcg gatggcagat attttcatgc cccgcaaatc ttcaaatttc
    36061 atttccgtca ggcattcaac cgcaatactt tgatacaatg gctcctgatg ttttattttt
    36121 tccgcttctt tgtcactggc aaccaccatc actaattccg ccgacaaata atggtccgcg
    36181 ccgttgttgt catgcagcgt gacaatggtt tccggcagcg tcacaaacac gcttttgctt
    36241 tcatccaggg tttccaccgc ttccgtccgc gcaacggcag tcggttttcc ttttccggca
    36301 taatgatgcc agacacccca tccagctcca gcgccgacca ccaacgccag aacggcgctg
    36361 accacgcccg ccaccacgat tttcttcata ttgcctttac actttgatca aaacggattc
    36421 gttctgttga aattgaggcg catcatcaag ctgtaaggcg gcactcacct cttcctgctg
    36481 atggcccgac tgttgttgct gttgcgactg cccttcggag gagacctgta cgttcacctc
    36541 cataaaattt tgcgccgtca gatgctggcg gagatcgtcg ctaaactgct gtaacgcgcg
    36601 gcaaacttca ctttggttcg cgccgatgtg caccatcagt ttgccgttgt cgagttgtac
    36661 ggcgatctcg agcttgccaa gcgacggtgg atcgagacgg atggtggaga tctgctgttg
    36721 tttgttgagc tgaaagtgga tctgatcttt caacagcgtc gtcagttttt cgcccagttc
    36781 gtccatcgaa agcgtggctg gtttggcgac aatctcctga tgttcgctga ccagatccgg
    36841 cgtcttaacc tgcaacaccg cacgcgcaat aaccggttcg ctgtccggca ctttttccgt
    36901 tttacgctcg acctgcgggc gcgcgttcac acgggttagt tcaggtttcg ggcgagctgg
    36961 ctgtgttgag acgcgcggct gcgtcggtgc aatggcatgt aaatcttcac tggcgtaagt
    37021 cgccagtctg gcctgctgtt cgggtttcgc ctgcggcaac tgactgatca gctcctgcaa
    37081 ctgcggcggc aacggcgtga gttcctgctg ctgcgtcggg cgttgcgccg cgttttgcac
    37141 caccgctttc gtcaattgct ggataaccgg cgatgttgcg acgtttcgcg gctgcggcgt
    37201 gtcctgatgc ggcgcggcag gttgcggaag taacagcgcc attagcgcct gcatcgccac
    37261 cggatcgttc tcctgcgggc gcgtcgcctg ttgctgactg gtttttgcgc tatgaacgcg
    37321 ctccgccacc gccgcgaccg ccattttcgg cagggtaaac gcgggcgcgt tttcactact
    37381 caccggcggt aaaatatccg ctttcagcga cgccgtttcc gcaagtgttc cgagcgtggc
    37441 aagcaatgcc ggattcatga cttctccgaa agtaaattaa caattgagta ggccagtacg
    37501 ccttcctgat gccgctgatg ctgttccata cgcgccttca acacttctgc acgctgctcg
    37561 cgccgtttaa tcagggcatt aaacgcgccg tacagctgcg ccttcaccgc acacaactgc
    37621 gccgcgtcgg tttccgccgc cacgtaatcg tgaaactggc gcatcaactg ctgatgcagt
    37681 gccggaaggc gtcgccagcg ttgcttgtcg agggcttcgt tcatcgcttc aaccagcgcg
    37741 aaaagttgcc tgcgttgttt ttccatcgtc ttaacccagc ttgcccgaaa gcccttccca
    37801 gccttcacgc aggttgctta agatcagcat cacttcatcg attttctctg ccgacagttc
    37861 gccactggct tcatacaagc gatacacgca gtggtcatag aggcgagaaa gattcaccac
    37921 cagctcgcca ccggtttcaa actccagcga actggtgagc gcattgagaa tgtcgatgca
    37981 tttattgatg ctctgggctt tcttctcgaa acgccgccct tcaatgtggc ttctggcgcg
    38041 ttccagctcg tccatcaggc cagaaaacag caccagcacc agttcaagag gcgatgccgc
    38101 agcggtacgc gccgcgagat cgacctcttt gtattgcgaa tagccatcct gtacttggta
    38161 catctttagg gatattcctt ctgagttacg cgaatgccgc catgctggct ttcatggtgt
    38221 acacctcaac cagcgtgttg gtgtactcct ccagataacg gtcgtagttg gcgttatagg
    38281 tatcggtaag ctgatcgcct tcgtcctgaa ttttgctcat ctgatcgtca atgttctgct
    38341 ggcgcagggt gatgatgccg ttgctggagt cggtataggt gtcaataagg ctgtccatct
    38401 gcgccaccat gctgttatcg ccgacgaaaa tagaggtcag gccgtcggga tcgctcttca
    38461 tcgcgtcgtt gaactggtct gaatcaatct gtaaatggcc gtgagaatcg agcgtaatgc
    38521 catagtcgac aatcgacacg ccgttgtagc tggcatgggc gatgtcatcg agctggtttg
    38581 ccagtgagct gagtccggcg tcgcctgcaa ataccccggc gctggtactg tcatcaccgt
    38641 gggtggtcag cgaatcaacc gtatcaatca aggtgttata tgcatcgaca aaggtctgta
    38701 ctttctcctg gctggcgctg gaatcttcgc tgatgttaaa ggtagtgaga tcgctgtcag
    38761 aatcgctgac ttcggtgaaa gtcatggtga cgccggggat cacatcatca aaggtattgc
    38821 tgctattagt gatctctggc cccgtggcgc tgccgagatg aataatcgcg tcctgggcgg
    38881 aggaaacatc cgtagcaaca ggcgcgctgg cgctgtcgtt gctggcgtca tgccccgtta
    38941 cgctcaccga aaatgcgctc tgcgccccgg tgctatcgga ggtgagcata atcgtggtgg
    39001 tgccgtcggt ttttaccagt gccgccgaca cgcccggatt gtcatcagag tcgttaatcg
    39061 cattcaccag ttcactggcg tcgataaaac cgtcgccgtc accgttttga tccgccgccg
    39121 acagatcgat atccatggtg ctgccgccca tcgtcagttc gaaggtgccg gtagcggaaa
    39181 aggcgtcgtc gcccatactg aacgtggttt gctgcccctg tgccagttgc tcaacgaaaa
    39241 atgagtagct tcccgcctgc gcctgggaat tggcggaaac ggtcgccgag tcattattgc
    39301 tggtggcggc aaacgtcacc gggccgtcag tatcgctgtt cagcgcgtca acggcgctct
    39361 gaaaatcgct caacgctgag ctgagcgagt ccaggccgct gctttccgca tccagctcgc
    39421 tctgcttctc ctgcaagttt gccgcctgag tggcaacatc cgcataggcg atttcttgcg
    39481 caatggttct tgggttgatc attgatgact ccggataacg atgtcaggtt attggcaaaa
    39541 ggcgtgccaa taatttttat gtgaataatc aataaattga aaaaggggcg gaaatggtgt
    39601 ttccggtctg gcggaaaaaa gcctgccgct gaaagcacaa caccgcccgc aggcggtgtt
    39661 gttcgtagcc ggataaggcg tttacgccgc atccggcaag tccgccatta ctgcagcagg
    39721 ctggaaacca tgctggacat gctgttggac tgtttcagca tggtgatgcc ggtctgcatc
    39781 agcacctgct gtttagtcat gttggacgct tccgtcgcgt agtcggtatc cataatgtta
    39841 ccgatagcga cttcggtgtt gtcctgcata ctggtcaggt tattagcggt gtcgttcaga
    39901 cggttgatag acgcccccag cttggactgg atctgcgaaa catcatccat cgcggtagaa
    39961 atgctggtga tcatctggtt agccgagcca gacgcagtca gctcagtacc gccagaaacg
    40021 cctgcggtgc cagtggtgtc agcctgatcc gcactgaagg agttgctgat agcagaaaga
    40081 tccgtcacca gggtattcag ctggcttgaa atattcacgg tcatggtgtc ggaagattcc
    40141 gcgcccacct ggaaagttac tgcgctctgg aacagaccgt cagtaccggt gttgctggta
    40201 ccggaaacac cgaacaggtt ggtgccgccg taagtggtgt tttgcagcat gtcagacatc
    40261 tgctgaccca gttcatcgta ttcgtcctgc atcgcctgca gatcgtcgtc gctgtaggtg
    40321 ccgttcgctg cctgagttga caggtctttc atacgaccca gcacatcaga catttcgtcg
    40381 aacatgctgt cagcggtttg cagcatcgcg gtggcatcat tgatgttgct cagcgccaca
    40441 cccataccgc tggactgtgc tgtcagacgg ttagcaattt gtttgcctgc ggcgtcatcg
    40501 gcagaagagt taatccggtt acccgttgcc aggcgttcca tagatgtaga cagagaagag
    40561 ctgcttttac tgattgcatt aacggcggcc attgaggcgt tattggtgtt aatagataac
    40621 atgatactgc tccttgggat taatcgtttt cgtttcgata ccctgtaaaa acgacacctc
    40681 aggagcaaac ttaaaatcgc cgttaaaatt ttttcgccac gtattatccg atgccacttt
    40741 tagctaaaaa aaagcagagt ttaaattcag ataaaaagat tattagcaat atctgccaca
    40801 tggccaggta tcaaaatatg tctttatcaa atgcttgata tagttaagat tgtcatcaat
    40861 tatcgcgttt tgttctcaat gtactgatgc caaaatttac attgcgttgc aaatagtaca
    40921 catttggcta ttgtacctgt cctgtcattt cgttacattt ccacgccata tagtgattaa
    40981 gaataatcct attaccataa aatattaatt atcacgcaga aaaatcatct tctcagggac
    41041 gtataagact gtgctttatg caaagcacta tcaggaaaca tttatccctt gctaaggtaa
    41101 actcaactat gagtattatt atcaacaact ggcggatgga cccgtcgctt aatgccttaa
    41161 tacattgcga aacgggtgaa acacgtcgtc tcggcgaata ccattttatt ttgctggaaa
    41221 cattagcaaa aaatgccgat gtggttttat cgcgatctta tttatgtgcc gaagtatgga
    41281 agaaccgcat agtcggcggt aacagcctgc cgacggcgat ccatgcgttg cgcgttgcta
    41341 tcgacgatga tggcaaacaa cagaacatta ttaaaacaat tccgaagaaa ggttacttgt
    41401 gcaataaaga gtatgtgtct ttgccggagt catcgcctgc agaagcgctg attattaccg
    41461 accagataca agatacagcc ccagaagaga tatcttcgac aacaccgcct gtacctgtta
    41521 gaaaaaaaca taaaggactg atggggttag cgttgaccgc ggctgttata tttattggct
    41581 cgaccgtggg gtattcacac ttgaaaagta cacctgacgc cccgcaactg gtaaaagagt
    41641 caattaacag tccaagaata aaaatatttc atcttagttc tggaaaggaa aataattccg
    41701 taccgttact gtcacaaacc ctcgaaccag gaaaagacaa actcgataat ttattgtcag
    41761 cgcataatat gacaatgaca acctattata aatatgttcg taaccgactg gaaagcgata
    41821 ttgttctgcg taaccagtgc aacggcagct ggcaattaac ctttaatgtg gaaggttggc
    41881 agaacagcga tattaatagc gcgatgtatc agaatttgga gaagttgtta aatactgtgc
    41941 agaaatgctg atgagtgcca atatatgccg gatgcgacgc taaacgcgtc ttatccggcc
    42001 taccgataat taccccaata aattcctcac cgtctggctg gaaattcgcg cctgaccgtt
    42061 cacagcctgc aataaaacct gatagttatg attggcgcta tcaagcacgc cgccgaggtt
    42121 ttccgacgcc tgcaccgcgt tttccgtttg cgggaagcgg gacatgccgt cgatcaattg
    42181 gcgggctttt tcctgctgca ccgccatctg cgcgcgctgc tgattaatgt tttccagcat
    42241 ttgagaaatc cccgccccgc cctgttgcag gctttgcgct aagcggtccg cctgtgacgg
    42301 ctcggcaaag gttttcagcg gcataaatgc tgatttatcg ccatcggtgc gcacgcttaa
    42361 gctgcgctcg atgtcaggcc actgtttttc agtggtggag aaactgatgc catccgcttg
    42421 ctgatgtacc tgcacgccca cgcggcgcag agcgttggtc agccgcgtct ggtactgcgc
    42481 ggcgttgtca tcctcagaca gcatcaccgc cgaaagctga gtctgccgcc cgtcagagac
    42541 gctgaacatg cgcgttcccg gcgtgggatt gtgtaccaga ttcgtcagat ccggcgagtg
    42601 aaaagtcacc cgcgcttccc cctgcaacac tggctgcaac tggcggtcca ccgcgccgcc
    42661 ggaaagcgcg gtgcgtttgt ccagcatctg catcagcgct gaactctgcg cctgcccgcc
    42721 cttacgctgg ctgtgacggt aatcgagcaa ctgctgttcc agttgcccga gatagtgatc
    42781 cgcctgctgc aacgtggtga gctgatcgtt aagctgcaca ctgtaacgct gcgggcgagt
    42841 ggtaatcagc ggcgaggccg gatattcact cgccgtcgcc gggagttttt gccgcaccgc
    42901 ctgagtgggc gcaacggttg acgacgatac cgcagcgcta tgcgcgccgc cggtggcgag
    42961 tgatgaagat gtaagtccga cctgcatacg gttcctgaaa gagttagaga acgctgaata
    43021 aattcaactc actcactttc gagtaagtct tgagggtagc ttccattgcc acttccagtc
    43081 cggtaaaggt gatcgacgcc gggccgtaat cgagatcctg taaagaacca atcaactcat
    43141 cattagaggt agaaatatcg gtctgcgcat cgctcagcat cgacatggtg ttctgcgtct
    43201 cgccaagcga cgcaatcgag gcgttgagat cgtccgaagc ggtatccacc acatcaacgg
    43261 cgttctgaag atcgctctgc acctgcggat ctgccggatc gacgtccggg ttttgcagct
    43321 cctgcgacag ggaattaagg gtgttgagca cgtctaaatt actgccgaag aaatcgctcg
    43381 ccgccacgtt ggtgtccact tccacgccgt tcgacacggt ggtctggcgg tgatcgctgt
    43441 tgccctggta gctgtagctg tcggtcacgc cgtcgccgtc atcgtccacc gccacaatcg
    43501 gcggctgatc gttgatggta ccgccaaaca cgtaatgccc gtcttcgtcc tgataattga
    43561 gcgccgccac catcgattcg gtgagcgact gaatatcctg cccgaggctt gcgagcgaat
    43621 cagcggtatt ggtgccgttc gccgcttcca gcaggtcgtc gcgcaccgcc agcaggctgt
    43681 tgttgacgcc atcgagaatc gactcctgct ggcttaaccc cgccgacgca gagtcaatat
    43741 tgctttgata ctgctcaatc gccgactgct ggcggttcaa ctgggtgata cgcgtggcgg
    43801 cgatcgggtc atccgacggc tgcaaaattt ccttgccggt tgccatctgc tcgaccacat
    43861 gcgccagaga gccggagaga ttattaaaac tttgcgtcat cgagacgtag gtttgttggg
    43921 tggttactcg cattttcgcg ctcccgctta gctgcacatt tccagcaccg aatcgaaaat
    43981 ctcggccccg gcggaaatga ctttcagatt agcttcataa atttgttgat aggtgatcag
    44041 gttcaccgct tcttcgtcca tgctgacgcc gctgacgctg ctctgctggt tttgcgcctc
    44101 agaataaaca ttggacgcgg catcgacttc cgtctggttt tgctggctgt aaatgccgat
    44161 gttgctgatg atcgacgagc acgcctgccc gaccgtcacg ctgccaaggt tggctatctc
    44221 cagcggttcg gtggagatgt tgatcagcgc ctgaaggttg tcgctgttac cgctttcatc
    44281 cggcgaactg gagaacgcca gctcatcggc ggtgatatcc gggttaacgg tcagcgggcc
    44341 atcggcgttg ctggcgtcat aaataaacag cggctcgccg gggttaccgt tgagatcgta
    44401 gccctgcgcc agctggttgt tgaccgcatc ggcaaactgc aacgccatgc tgttgatggt
    44461 gtcggtcagc ggcgtcagca cgtcgttttg ataatcaaac agtgcgccta atgaaccgcc
    44521 ggtgtcggta gtcatcgtcg aggtggtgcc agcgaaagtc agcgacatgg tcggcgtgcc
    44581 atcggcgttg gtttccagcg cgatggtcga gctttgctgc ccgctcacca gcggttgacc
    44641 gtttttcagg gtgacgttgt agttgccctg gtcgtcgata ttgacctgca catccatcat
    44701 cccgctcagt tcttccacca tctgatcgcg ggcgtcgtac agcgcggagg cgttatcgcc
    44761 gttggcttcg gcctgggcga tttgctggtt atagctggcg atgccgctgg taagcgtgtt
    44821 gatttgcgac accattgcct gctgctggct gatgatttcc gtgctttgcg agtcgatgta
    44881 atccagcgtg ttgtcgatac gcaacgacag cgccccggct tcgctgatca cctgttcgcg
    44941 cagggcggaa tcatcggggc tggtggtcgc ttcgttgagg gcggcgaaga agttatcgaa
    45001 gccgccgctc aggctgctgt tatcgtcgct cagtaccgct tccagttgcg tgagataccc
    45061 ctgctgggtg ctgtaatagc cgtaatcgct ggcggcatac cacacctgat tcacctgata
    45121 ctggttagag acgcggcgaa tgctgtcgac ctgcacgccg ttaccggcgc tgttcggcga
    45181 accaccgctg gcgccgatgg tgctgatctc cgccacctga cgggtgtagc ccgtggtcat
    45241 ggcgttggcg gtgttttgcg ccgtgacgtt cagctctacc tgcgcggttg atgcgccgct
    45301 gtagccgatg ttaatcatgt ccatgttgga tccttaccgg taaatcgtgc cggttaaaag
    45361 cggcgtgaag atggggaatc ttaagttccg taaaccgggt gccagacgcc ggatgcagcc
    45421 tgcatacggg cacaaacata aaaatatgtg cgaactcgta ggcct
//
LOCUS       DAMZFT010000020        44038 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.020, whole genome shotgun sequence.
ACCESSION   DAMZFT010000020 DAMZFT010000000
VERSION     DAMZFT010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 44038)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44038)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44038
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.020"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            321..1220
                     /gene="lysO"
                     /locus_tag="PN977_001296"
     CDS             321..1220
                     /gene="lysO"
                     /locus_tag="PN977_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lysine exporter LysO"
                     /protein_id="HDI6041348.1"
                     /translation="MFSGLLIILVPLIVGYLIPLRQKAALRVINQLLSWMVYLILFFM
                     GISLAFLDNLASNLLAILHYSAVSITVILLCNIAALMWLERGLPWRNHHHQEKLPSRI
                     AMALESLKLCGVVVIGFAIGLSGLAFLQHATEASEYTLILLLFLVGIQLRNNGMTLKQ
                     IVLNRRGMIVAVVVVASSLIGGLINAFILDLPINTALAMASGFGWYSLSGILLTESFG
                     PVIGSAAFFNDLARELIAIMLIPGLIRRSRSTALGLCGATSMDFTLPVLQRTGGLDMV
                     PAAIVHGFILSLLVPILIAFFSA"
     gene            1364..3016
                     /gene="hcp"
                     /locus_tag="PN977_001297"
                     /gene_synonym="priS"
     CDS             1364..3016
                     /gene="hcp"
                     /locus_tag="PN977_001297"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HDI6041349.1"
                     /translation="MFCVQCEQTIRTPAGNGCSYAQGMCGKTAETSDLQDLLIAALQG
                     LSAWAVKAREYGIINHDVDSFAPRAFFSTLTNVNFDSPRIVGYAREAIALREALKAQC
                     LAVEANARVDNPMADLQLVSDDLGELQRQAAEFTPNKDKAAIGENILGLRLLCLYGLK
                     GAAAYMEHAHVLGQYDNDIYAQYHKIMAWLGTWPADMNALLECSMEIGQMNFKVMSIL
                     DAGETSKYGHPTPTQVNVKATAGKCILISGHDLKDLYNLLEQTEGTGVNVYTHGEMLP
                     AHGYPELRKFKHLVGNYGSGWQNQQVEFARFPGPIVMTSNCIIDPTVGAYDDRIWTRS
                     IVGWPGVRHLDGEDFSAVIAQAQQMAGFPYSEIPHLITVGFGRQTLLGAADTLIDLVS
                     REKLRHIFLLGGCDGARGERHYFTDFATSVPDDCLILTLACGKYRFNKLEFGDIEGLP
                     RLVDAGQCNDAYSAIILAVTLAEKLGCGVNDLPLSLVLSWFEQKAIVILLTLLSLGVK
                     NIVTGPTAPGFLTPDLLAVLNEKFGLRSITTVEEDMKQLLSA"
     gene            3028..3996
                     /gene="hcr"
                     /locus_tag="PN977_001298"
     CDS             3028..3996
                     /gene="hcr"
                     /locus_tag="PN977_001298"
                     /EC_number="1.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidoreductase"
                     /protein_id="HDI6041350.1"
                     /translation="MTMPTNQCPWRMQVHHITQETPDVWTISLICHDYYPYRAGQYAL
                     VSVRNSAETLRAYTISSTPGVSEYITLTVRRIDDGVGSRWLTRDVKRGDYLWLSDAMG
                     EFTCDDKAEDKFLLLAAGCGVTPIMSMRRWLAKNRPQADVQVIYNVRTPQDVIFADEW
                     RNYPVTLVAENNVTEGFIAGRLTRELLAGVPDLASRTVMTCGPAPYMDWVEQEVKALG
                     VTRFFKEKFFTPVAEAATSGLKFTKLQPAREFYAPVGTTLLEALESNNVPVVAACRAG
                     VCGCCKTKVVSGEYTVSSTMTLTDAEIAEGYVLACSCHPQGDLVLA"
     gene            4129..5847
                     /gene="poxB"
                     /locus_tag="PN977_001299"
     CDS             4129..5847
                     /gene="poxB"
                     /locus_tag="PN977_001299"
                     /EC_number="1.2.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone-dependent pyruvate dehydrogenase"
                     /protein_id="HDI6041351.1"
                     /translation="MKQTVAAYIAKTLESAGVKRIWGVTGDSLNGLSDSLNRMGTIEW
                     MSTRHEEVAAFAAGAEAQLSGELAVCAGSCGPGNLHLINGLFDCHRNHVPVLAIAAHI
                     PSSEIGSGYFQETHPQELFRECSHYCELVSSPEQIPQVLAIAMRKAVLNRGVSVVVLP
                     GDVALKPAPEGATTHWYHAPQPVVTPEEEELRKLAQLLRYSSNIALMCGSGCAGAHKE
                     LVEFAGKIKAPIVHALRGKEHVEYDNPYDVGMTGLIGFSSGFHTMMNADTLVLLGTQF
                     PYRAFYPTDAKIIQIDINPASIGAHSKVDMALVGDIKSTLRALLPLVEEKTDRKFLDK
                     ALEDYRDARKGLDDLAKPSEKAIHPQYLAQQISHFAADDAIFTCDVGTPTVWAARYLK
                     MNGKRRLLGSFNHGSMANAMPQALGAQATEPERQVVAMCGDGGFSMLMGDFLSVMQMK
                     LPVKIIVFNNSVLGFVAMEMKAGGYLTDGTELHDTNFARIAEACGITGIRVEKASEVD
                     EALQRAFSIDGPVLVDVVVAKEELAIPPQIKLEQAKGFSLYMLRAIISGRGDEVIELA
                     KTNWLR"
     gene            5884..6885
                     /gene="ltaE"
                     /locus_tag="PN977_001300"
     CDS             5884..6885
                     /gene="ltaE"
                     /locus_tag="PN977_001300"
                     /EC_number="4.1.2.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low-specificity L-threonine aldolase"
                     /protein_id="HDI6041352.1"
                     /translation="MIDLRSDTVTRPSRAMLEAMMAAPVGDDVYGDDPTVNALQDYAA
                     ELSGKEAAIFLPTGTQANLVALLSHCERGEEYIVGQAAHNYLFEAGGAAVLGSIQPQP
                     IDAAADGTLPLDKVAMKIKPDDIHFARTKLLSLENTHNGKVLPREYLKEAWEFTRERN
                     LALHVDGARIFNAVVAYGCELKEITQYCDSFTICLSKGLGTPVGSLLVGNRDYIKRAI
                     RWRKMTGGGMRQSGILAAAGIYALKNNVARLQEDHDNAAWMAEQLREAGADVMRQDTN
                     MLFVRVGEENAAALGEYMKARNVLINASPIVRLVTHLDVSRAQLAEVAAHWRAFLAR"
     gene            6896..8326
                     /locus_tag="PN977_001301"
     CDS             6896..8326
                     /locus_tag="PN977_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2867 domain-containing protein"
                     /protein_id="HDI6041353.1"
                     /translation="MPQRILVLGASGYIGQHLVRTLSQQGHQILAAARHVDRLAKLQL
                     ANVSCHKVDLSWPDNLPALLQDIDTVYFLVHSMGEGGDFIAQERQVALNVRDALREVP
                     VKQLIFLSSLQAPPHEQSDHLRARQATADILREAGVPVTELRAGIIVGAGSAAFEVMR
                     DMVYNLPVLTPPRWVRSRTTPIALENLLHYLVALLDHPASEHRIFEAAGPEVLSYQQQ
                     FEHFMAVSGKRRWLIPIPFPTRWISVWFLNVITSVPPTTAKALIQGLKHDLLADDTAL
                     RALIPQRLIAFDDAVRRTLKEEEKLVNSSDWGYDAQAFARWRPEYGYFAKQAGFTVKT
                     SASLAALWQVVNQIGGRERYFFGNILWQTRALMDRAIGHKLAKGRPEREYLQTGDAVD
                     SWKVIIVEPEKQLALLFGMKAPGLGRLCFTLEDKGDYRTIDVRAFWHPHGMPGLFYWL
                     LMIPAHLFIFRGMAKRIARLAEQSTD"
     gene            8425..9438
                     /locus_tag="PN977_001302"
     CDS             8425..9438
                     /locus_tag="PN977_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HDI6041354.1"
                     /translation="MKVLVTGATSGLGRNAVEFLCQKGISVRATGRNEAMGKLLEKMG
                     AEFVPADLTELVSSQAKVMLAGIDTLWHCSSFTSPWGTQQAFDLANVRATRRLGEWAV
                     AWGVRNFIHISSPSLYFDYHHHRDIKEDFRPHRFANEFARSKAASEEVINMLSQANPQ
                     TRFTILRPQSLFGPHDKVFIPRLAHMMHHYGSILLPHGGSALVDMTYYENAVHAMWLA
                     SQEACDKLPSGRVYNITNGEHRTLRSIVQKLIDELNIDCRIRSVPYPMLDMIARSMER
                     LGRKSAKEPPLTHYGVSKLNFDFTLDITRAQEELGYQPVLTLDEGIEKTAAWLRDHGK
                     LPR"
     gene            complement(9435..10265)
                     /gene="amiD"
                     /locus_tag="PN977_001303"
     CDS             complement(9435..10265)
                     /gene="amiD"
                     /locus_tag="PN977_001303"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiD"
                     /protein_id="HDI6041355.1"
                     /translation="MRRVFWLVAAALLLAGCAGEKGIVEKEGYQLDTRRQAQAAYPRI
                     KVMVIHYTADDFDSSLATLTDKQVSSHYLVPAVPPRYNGKPRIWQLVPEQELAWHAGI
                     SAWRGATRLNDTSIGIELENRGWQKSAGVKYFAPFEPAQIQALIPLAKDIIARYHIKP
                     ENVVAHADIAPQRKDDPGPLFPWQQLAQQGIGAWPDAQRVNFYLAGRAPHTPVDTASL
                     LELLARYGYDVKPDMTPREQRRVIMAFQMHFRPTLYNGEADAETQAIAEALLEKYGQD
                     "
     gene            complement(10262..10585)
                     /locus_tag="PN977_001304"
     CDS             complement(10262..10585)
                     /locus_tag="PN977_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HDI6041356.1"
                     /translation="MQFSTTPTLEGQTIVEYCGVVTGEAILGANIFRDFFAGIRDIVG
                     GRSGAYEKELRKAREIAFEELGDQARALGADAVVGIDIDYETVGQNGSMLMVSVSGTA
                     VKTRR"
     gene            complement(10796..12598)
                     /locus_tag="PN977_001305"
     CDS             complement(10796..12598)
                     /locus_tag="PN977_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016243277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HDI6041357.1"
                     /translation="MEYLFPWVIFNMINNISDQASSFPGTQLNQSDNFLDSLREFFAI
                     LNSSRKGELSTWDTIYLHLILAINADSDLIKNDELLAENITSANYQFNIFFSNAFEID
                     VKKYLGKSEDNEVEIKVGNERITIGIRNVSNGKLERQKFLFPLDYENKLQHQLDKHFT
                     IESHPLLYRHTMGSKIANVIFEKLYSRIDFNKEQYISFIKDAFIHFYDYSRRYAISEN
                     IAKDAVTNNIALMSTFYDSENTSGEVLNNDFTEEESFETALNIEHAIVLGFADDNFET
                     KPVHYQDLLTRFSAFQDTVFNLFPEMHSSHYNDICSVSVDMTKGTQCMIHLMVNEEVL
                     MSLPVPVAMMVREDATNLVNLKTLLNDGCFIKYSHFNDVALIKQNINNLYLSHTIVNE
                     SILKKCCFENGSLGDVKITNSNVINSVFKNISFRSVKINNVNTHSLKFINCHFFNVDM
                     IRVNFSKCLFHDCSMHGVKIKPWLPVKWTKELINDYLYGCLLSLYSICARDIYNMTAG
                     NNVKVAADAFLEILYFLKNKYCIKLLSAQDRAFIYEFARMIFAYINDKSIEILLLSCF
                     AAADQKAIQRYIPQAQDGEDFRNHLQYKLALPTH"
     gene            13213..13728
                     /locus_tag="PN977_001306"
     CDS             13213..13728
                     /locus_tag="PN977_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HDI6041358.1"
                     /translation="MRYRSLSLLIPCALLLSACTTVTPAYKDNGPRTGSCAQGGPDSV
                     AQQFYDYRIQHRNNDITALRPYLSDKLATLLSDASRDNSHRELLSSDPFSSRTTLPDS
                     AHVASASTIPNRDARNIPLRVDLKQGDQGWQDEVLMIQEGQCWVIDDVRYLGGSVHAT
                     AGTLRQSIENR"
     gene            13946..14674
                     /gene="artP"
                     /locus_tag="PN977_001307"
     CDS             13946..14674
                     /gene="artP"
                     /locus_tag="PN977_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter ATP-binding protein
                     ArtP"
                     /protein_id="HDI6041359.1"
                     /translation="MSIQLNGINCFYGAHQALFDITLDCPQGETLVLLGPSGAGKSSL
                     LRVLNLLEMPRSGTLNIAGNHFDFTKTPSDKAIRDLRRNVGMVFQQYNLWPHLTVQQN
                     LIEAPCRVLGLSKDQALARAEKLLERLRLKPYSDRYPLHLSGGQQQRVAIARALMMEP
                     QVLLFDEPTAALDPEITAQIVSIIRELAETNITQVIVTHEVEVARKTASRVVYMENGH
                     IVEQGDASCFTEPQTEAFKNYLSH"
     gene            14692..15423
                     /gene="artJ"
                     /locus_tag="PN977_001308"
     CDS             14692..15423
                     /gene="artJ"
                     /locus_tag="PN977_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter substrate-binding
                     protein"
                     /protein_id="HDI6041360.1"
                     /translation="MKKVLIAALIAGFSLSATAAETIRFATEASYPPFESIDANNQIV
                     GFDVDLAQALCKEIDATCTFSNQAFDSLIPSLKFRRVEAVMAGMDITPEREKQVLFTT
                     PYYDNSALFVGQQGKYTSVDQLKGKKVGVQNGTTHQKFIMDKHPEITTVPYDSYQNAK
                     LDLQNGRIDSVFGDTAVVTEWLKDNPKLAAVGDKVTDKDYFGTGLGIAVRQGNTELQQ
                     KLNTALEKVKKDGTYETIYNKWFQK"
     gene            15430..16146
                     /gene="artQ"
                     /locus_tag="PN977_001309"
     CDS             15430..16146
                     /gene="artQ"
                     /locus_tag="PN977_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtQ"
                     /protein_id="HDI6041361.1"
                     /translation="MNEFFPLASAAGMTVGLAVCALIVGLALAMFFAVWESAKWRPVA
                     WAGSALVTILRGLPEILVVLFIYFGSSQLLLTLSDGFTINLGFVQIPVQMDIENFDVS
                     PFLCGVIALSLLYAAYASQTLRGALKAVPVGQWESGQALGLSKSAIFFRLVMPQMWRH
                     ALPGLGNQWLVLLKDTALVSLISVNDLMLQTKSIATRTQEPFTWYIVAAAIYLVITLL
                     SQYILKRIDLRATRFERRPS"
     gene            16146..16814
                     /gene="artM"
                     /locus_tag="PN977_001310"
     CDS             16146..16814
                     /gene="artM"
                     /locus_tag="PN977_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtM"
                     /protein_id="HDI6041362.1"
                     /translation="MFEYLPELMKGLHTSLTLTVASLIVALILALIFTIILTLKTPVL
                     VWLVRGYITLFTGTPLLVQIFLIYYGPGQFPTLQEYPALWHLLSEPWLCALIALSLNS
                     AAYTTQLFYGAIRAIPEGQWQSCSALGMSKKDTLAILLPYAFKRSLSSYSNEVVLVFK
                     STSLAYTITLMEVMGYSQLLYGRTYDVMVFGAAGIIYLIVNGLLTLMMRLIERKALAF
                     ERRN"
     gene            17040..17771
                     /gene="artJ"
                     /locus_tag="PN977_001311"
     CDS             17040..17771
                     /gene="artJ"
                     /locus_tag="PN977_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein ArtJ"
                     /protein_id="HDI6041363.1"
                     /translation="MKKLVLAALLSSCAFGASAAEKINFGVSATYPPFESIGANNEIV
                     GFDIDLAKALCKQMQAECTFTNHAFDSLIPSLKFRKYDAVISGMDITPERSKQVSFTT
                     PYYENSAVVIAKKNTYKTFADLKGKRIGMENGTTHQKYIQDQHPEVKTVSYDSYQNAF
                     IDLKNGRIDGVFGDTAVVNEWLKTNPQLGVATEKVTDPQYFGTGLGIAVRPDNKALLE
                     KLNNALAAIKADGTYQKINDQWFPQ"
     gene            complement(17800..18927)
                     /gene="rlmC"
                     /locus_tag="PN977_001312"
     CDS             complement(17800..18927)
                     /gene="rlmC"
                     /locus_tag="PN977_001312"
                     /EC_number="2.1.1.189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(747)-C(5))-methyltransferase
                     RlmC"
                     /protein_id="HDI6041364.1"
                     /translation="MQCALYDAGRCRSCQWITQPIPEQLSAKTADLKNLLADFPVEEW
                     CAPVSGPEQGFRNKAKMVVSGSVEKPLLGMLHRDGTPEDLCDCPLYPASFAPVFAALK
                     PFIARAGLTPYNVARKRGELKYILLTESQSDGGMMLRFVLRSDTKLAQLRKALPWLQE
                     QLPQLKVITVNIQPVHMAIMEGETEIYLTEQQALAERFNDVPLWIRPQSFFQTNPAVA
                     SQLYATARDWVRQLPVNHMWDLFCGVGGFGLHCATPDIQLTGIEIAPEAIACAKQSAA
                     ELGLTRLQFQALDSTQFATAQGEVPELVLVNPPRRGIGIPLCDYLSTMAPRFIIYSSC
                     NAQTMAKDIRELPGYRIERVQLFDMFPHTAHYEVLTLLVKQ"
     gene            complement(18968..19456)
                     /locus_tag="PN977_001313"
     CDS             complement(18968..19456)
                     /locus_tag="PN977_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjO family protein"
                     /protein_id="HDI6041365.1"
                     /translation="MEDETLGFFKKTSSSHARLNVPALVQVAALAIIMIRGLDVLMIF
                     NTLGVRGIGEFIHRSVQTWSLTLVFLSSLVLVFIEIWCAFSLVKGRRWARWLYLLTQI
                     TAASYLWAASLGYGYPELFSIPGESKREIFHSLILQKLPDMLILMLLFVPSTSRRFFQ
                     LQ"
     gene            complement(19516..20361)
                     /gene="potI"
                     /locus_tag="PN977_001314"
     CDS             complement(19516..20361)
                     /gene="potI"
                     /locus_tag="PN977_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotI"
                     /protein_id="HDI6041366.1"
                     /translation="MNNLPVVRSPWRIVILLLGFTFLYAPMLMLVIYSFNSSKLVTVW
                     AGWSTRWYGELLRDDAMMSAVGLSLTIAACAATAAAILGTIAAVVLVRFGRFRGSNGF
                     AFMITAPLVMPDVITGLSLLLLFVALAHAIGWPADRGMLTIWLAHVTFCTAYVAVVIS
                     SRLRELDSSIEEAAMDLGATPLKVFFVITLPMIMPAIISGWLLAFTLSLDDLVIASFV
                     SGPGATTLPMLVFSSVRMGVNPEINALATLILGAVGIVGFIAWYLMARAEKQRIRDIQ
                     RARRG"
     gene            complement(20358..21311)
                     /gene="potH"
                     /locus_tag="PN977_001315"
     CDS             complement(20358..21311)
                     /gene="potH"
                     /locus_tag="PN977_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotH"
                     /protein_id="HDI6041367.1"
                     /translation="MSTLEPAAQSKPPGGFKLWLSQLQMKHGRKLVIALPYIWLILLF
                     LLPFLIVFKISLAEMARAIPPYTELMEWADGQLSITLNLGNFLQLTDDPLYFDAYLQS
                     LQVAAISTICCLLIGYPLAWAVAHSKPSTRNILLLLVILPSWTSFLIRVYAWMGILKN
                     NGVLNNFLLWLGVIDQPLTILHTNLAVYIGIVYAYVPFMVLPIYTALIRIDYSLVEAA
                     LDLGARPLKTFFTVIVPLTKGGIIAGSMLVFIPAVGEFVIPELLGGPDSIMIGRVLWQ
                     EFFNNRDWPVASAVAIIMLLLLIVPIMWFHKHQQKSVGEHG"
     gene            complement(21321..22454)
                     /gene="potG"
                     /locus_tag="PN977_001316"
     CDS             complement(21321..22454)
                     /gene="potG"
                     /locus_tag="PN977_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000996010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter ATP-binding subunit
                     PotG"
                     /protein_id="HDI6041368.1"
                     /translation="MNDAIPRPQAKTRKALTPLLEIRNLTKSYDGQHAVDDVSLTIYK
                     GEIFALLGASGCGKSTLLRMLAGFEQPSAGQIMLDGVDLSQVPPYLRPINMMFQSYAL
                     FPHMTVEQNIAFGLKQDKLPKAEIASRVNEMLGLVHMQEFAKRKPHQLSGGQRQRVAL
                     ARSLAKRPKLLLLDEPMGALDKKLRDRMQLEVVDILERVGVTCVMVTHDQEEAMTMAG
                     RIAIMNRGKFVQIGEPEEIYEHPTTRYSAEFIGSVNVFEGVLKERQEDGLVLYSPGLV
                     HPLKVDADASVVDNVPVHVALRPEKIMLCEEPPANGCNFAVGEVIHIAYLGDLSVYHV
                     RLKSGQMISAQLQNAHRHRKGLPTWGDEVRLCWEVDSCVVLTV"
     gene            complement(22549..23661)
                     /gene="potF"
                     /locus_tag="PN977_001317"
     CDS             complement(22549..23661)
                     /gene="potF"
                     /locus_tag="PN977_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotF"
                     /protein_id="HDI6041369.1"
                     /translation="MTALNKKWLSGLVAGALMAVSVSTLAAEQKTLHIYNWSDYIAPD
                     TVANFEKETGIKVVYDVFDSNEVLEGKLMAGSTGFDLVVPSASFLERQLTAGVFQPLD
                     KSKLPEWKNLDPELLKLVAKHDPDNKFAMPYMWATTGIGYNVDKVKAVLGENAPVDSW
                     DLILKPENLEKLKSCGVSFLDAPEEVFATVLNYLGKDPNSTKADDYTGPATDLLLKLR
                     PNIRYFHSSQYINDLANGDICVAIGWAGDVWQASNRAKEAKNGVNVSFSIPKEGVMAF
                     FDVFAMPADAKNKDEAYQFLNYLLRPDVVAHISDHVFYANANKEATPLVSAEVRDNPG
                     IYPPADVRAKLFTLKVQDPKIDRVRTRAWTKVKSGK"
     gene            complement(24012..24488)
                     /locus_tag="PN977_001318"
     CDS             complement(24012..24488)
                     /locus_tag="PN977_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HDI6041370.1"
                     /translation="MTSLVVPGLDTLRQWLDDLGMSFFECDNCQALHLPHMQNFDGVF
                     DAKIDLIDNTILFSAMAEVRPSAVLPLAADLSAINASSLTVKAFLDMQDDNLPKLVVC
                     QSLSVMQGVTYEQFAWFVRQSEEQISMVILEANAHQLLLPTDDEGQNNVTENYFLH"
     gene            complement(24576..25478)
                     /gene="rimK"
                     /locus_tag="PN977_001319"
     CDS             complement(24576..25478)
                     /gene="rimK"
                     /locus_tag="PN977_001319"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6--L-glutamate ligase"
                     /protein_id="HDI6041371.1"
                     /translation="MKIAILSRDGTLYSCKRLREAAIQRGHLVEILDPLSCYMNINPA
                     ASSIHYKGRKLPHFDAVIPRIGTAITFYGTAALRQFEMLGSYPLNESVAIARARDKLR
                     SMQLLARQGIDLPVTGIAHSPDDTSDLIDMVGGAPLVVKLVEGTQGIGVVLAETRQAA
                     ESVIDAFRGLNAHILVQEYIKEAQGCDIRCLVVGDEVVAAIERRAKEGDFRSNLHRGG
                     VASVARITPQEREIAIKAARTMALDVAGVDILRANRGPLVMEVNASPGLEGIEKTTGI
                     DIAGKMIRWIERHATTEYCLKTGG"
     gene            complement(25539..26261)
                     /gene="nfsA"
                     /locus_tag="PN977_001320"
     CDS             complement(25539..26261)
                     /gene="nfsA"
                     /locus_tag="PN977_001320"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase NfsA"
                     /protein_id="HDI6041372.1"
                     /translation="MTPTIELICGHRSIRHFTDEPISEAQREAIINSARATSSSSFLQ
                     CSSIIRITDKALRDELVTLTGGQKHVAQAAEFWVFCADFNRHLQICPDAQLGLAEQLL
                     LGVVDTAMMAQNALTAAESLGLGGVYIGGLRNNIEAVTELLKLPRHVLPLFGLCLGWP
                     ADNPDLKPRLPASILVHENSYQPLDKDALAQYDEQLAECYLTRGSNNRRDTWSDHIRR
                     TIIKESRPFILDYLHKQGWATR"
     gene            complement(26245..26532)
                     /locus_tag="PN977_001321"
     CDS             complement(26245..26532)
                     /locus_tag="PN977_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjC family protein"
                     /protein_id="HDI6041373.1"
                     /translation="MRAIGKLPKSVLILEFIGMMSLAVALLSVSDPLSLPEPFSRPEV
                     QILMIFLGVLLMLPAAVVVILQVVKRLAPQLMNRPPQYSRSEREKDNDANH"
     gene            26692..26949
                     /gene="grxA"
                     /locus_tag="PN977_001322"
     CDS             26692..26949
                     /gene="grxA"
                     /locus_tag="PN977_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 1"
                     /protein_id="HDI6041374.1"
                     /translation="MQTVIFGRPGCPYCVRAKDLAEKLSNERDDFQYQYVDIRAEGIT
                     KEDLQQKAGKPVETVPQIFVDQQHIGGYTDFAAWVKENLDA"
     gene            complement(26979..27356)
                     /locus_tag="PN977_001323"
     CDS             complement(26979..27356)
                     /locus_tag="PN977_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YbjM"
                     /protein_id="HDI6041375.1"
                     /translation="MKHKQRWAGAICCFVLFIVVCLFLATHMKGAFRAAGHPEIGLLF
                     FILPGAVASFFSQRREVLKPLFGAMLAAPCSMLIMRLFFSPTRSFWQELAWLLSAVFW
                     CALGALCFLFISSLFKPQHRKNQ"
     gene            27626..29311
                     /locus_tag="PN977_001324"
     CDS             27626..29311
                     /locus_tag="PN977_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate:alanine antiporter"
                     /protein_id="HDI6041376.1"
                     /translation="MNINVAELLNGNYILLLFVVLALGLCLGKLRLGSIQLGNSIGVL
                     VVSLLLGQQHFSINTDALNLGFMLFIFCVGVEAGPNFFSIFFRDGKNYLMLALVMVGS
                     ALVIALGLGKLFGWDIGLTAGMLAGSMTSTPVLVGAGDTLRHSGMESRQLSLALDNLS
                     LGYALTYLIGLVSLIVGARYLPKLQHQDLQTSAQQIARERGLDTDANRKVYLPVIRAY
                     RVGPELVAWTDGKNLRELGIYRQTGCYIERIRRNGILANPDGDAVLQMGDEIALVGYP
                     DAHARLDPSFRNGKEVFDRDLLDMRIVTEEVVVKNHNAVGKRLAQLKLTDHGCFLNRV
                     IRSQIEMPIDDNVVLNKGDVLQVSGDARRVKTIADRIGFISIHSQVTDLLAFCAFFVI
                     GLMIGMITFQFSTFSFGMGNAAGLLFAGIMLGFMRANHPTFGYIPQGALSMVKEFGLM
                     VFMAGVGLSAGSGINNGLGAIGGQMLIAGLIVSLVPVVICFLFGAYVLRMNRALLFGA
                     MMGARTCAPAMEIISDTARSNIPALGYAGTYAIANVLLTLAGTIIVMVWPGLG"
     gene            complement(29486..30022)
                     /gene="rcdA"
                     /locus_tag="PN977_001325"
     CDS             complement(29486..30022)
                     /gene="rcdA"
                     /locus_tag="PN977_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RcdA"
                     /protein_id="HDI6041377.1"
                     /translation="MRRANDPQRREKIIQATLEAVKLYGIHAVTHRKIATLAGVPLGS
                     MTYYFSGIDELLLEAFSRFTEIMSRQYQAFFSDVSDAPGACQAITDMIYSSQVATPDN
                     MELMYQLYALASRKPLLKTVMQNWMQRSQQTLEQWFEPGTARALDAFIEGMTLHFVTD
                     RKPLSREEILRMVERVAG"
     gene            30106..31314
                     /locus_tag="PN977_001326"
     CDS             30106..31314
                     /locus_tag="PN977_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDI6041378.1"
                     /translation="MTVNSSRNALKRRTWALFMFFFLPGLLMASWATRTPAIRDILSV
                     SIAEMGGVLFGLSIGSMSGILCSAWLVKRFGTRNVILVTMSCALIGMMILSLALWLTS
                     PLLFAVGLGVFGASFGSAEVAINVEGAAVEREMNKTVLPMMHGFYSLGTLAGAGVGMA
                     LTAFGVPATVHILLAALVGIAPIYIAIQAIPDGTGKNAADGTQHGEKGVPFYRDIQLL
                     LIGVVVLAMAFAEGSANDWLPLLMVDGHGFSPTSGSLIYAGFTLGMTVGRFTGGWFID
                     RYSRVAVVRASALMGALGIGMIIFVDSAWVAGVSVVLWGLGASLGFPLTISAASDTGP
                     DAPTRVSVVATTGYLAFLVGPPLLGYLGEHYGLRSAMLVVLALVILAAIVAKAVAKPD
                     TKTQTAMENS"
     gene            31314..32129
                     /gene="ybjI"
                     /locus_tag="PN977_001327"
     CDS             31314..32129
                     /gene="ybjI"
                     /locus_tag="PN977_001327"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415365.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase"
                     /protein_id="HDI6041379.1"
                     /translation="MSIKLIAVDMDGTFLSDQKTYNRERFMAQYQQMKAQGIRFVVAS
                     GNQYYQLISFFPEIANEIAFVAENGGWVVSEGKDVFNGELSKDAFATVVEHLLTRPEV
                     EIIACGKNSAYTLKKYDDAMKTVAEMYYHRLEYVDNFDNLEDIFFKFGLNLSDELIPQ
                     VQKALHEAIGDIMVPVHTGNGSIDLIIPGVHKANGLRQLQKLWGIDDSEVVVFGDGGN
                     DIEMLRQAGFSFAMENAGSAVVAAAKYRAGSNNREGVLDVIDKVLKHEAPFNQ"
     gene            32215..32499
                     /locus_tag="PN977_001328"
     CDS             32215..32499
                     /locus_tag="PN977_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6041380.1"
                     /translation="MIMKNCLLLGALLMGFTGVAMAQSVTVDVPSGYKVVVVPDSVSV
                     PQAVSVATVPQTVYVAPAPAPAYRPHPYVRHLASVGEGMVIEHQIDDHHH"
     gene            complement(32540..33772)
                     /gene="mdfA"
                     /locus_tag="PN977_001329"
     CDS             complement(32540..33772)
                     /gene="mdfA"
                     /locus_tag="PN977_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdfA"
                     /protein_id="HDI6041381.1"
                     /translation="MQNKLASGARLGRQALLFPLCLVLYEFSTYIGNDMIQPGMLAVV
                     EQYQAGIDWVPTSMTAYLAGGMFLQWLLGPLSDRIGRRPVMLAGVVWFIVTCLAILLA
                     QNIEQFTLLRFLQGISLCFIGAVGYAAIQESFEEAVCIKITALMANVALIAPLLGPLV
                     GAAWIHVLPWEGMFVLFAALAAISFFGLQRAMPETATRIGEKLSLKELGRDYKLVLKN
                     GRFVAGALALGFVSLPLLAWIAQSPIIIITGEQLSSYEYGLLQVPIFGALIAGNLLLA
                     RLTSRRTVRSLIIMGGWPIMIGLLVAAAATVISSHAYLWMTAGLSLYAFGIGLANAGL
                     VRLTLFASDMSKGTVSAAMGMLQMLIFTVGIEISKHAWLNGGNGLFNLFNLVNGILWL
                     LLMVIFLKDKQMGNSHEG"
     gene            34057..34653
                     /gene="ybjG"
                     /locus_tag="PN977_001330"
     CDS             34057..34653
                     /gene="ybjG"
                     /locus_tag="PN977_001330"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HDI6041382.1"
                     /translation="MLENLNLSLFSLINATPDSAPWMISLAIFIAKDLITVMPLLAAV
                     LWLWGLTAQRQLVIKIAIALAVSLFVSWTMGHLFPHDRPFVENIGYNFLHHAADDSFP
                     SDHGTVIFTFALAFLCWHRLWSGSLLMVLAVVIAWSRVYLGVHWPLDMLGGLLAGMIG
                     CLSAQIIWQAMGHKLYQRLQSWYRFCFALPIRKGWVRD"
     gene            34711..35469
                     /gene="deoR"
                     /locus_tag="PN977_001331"
     CDS             34711..35469
                     /gene="deoR"
                     /locus_tag="PN977_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor DeoR"
                     /protein_id="HDI6041383.1"
                     /translation="METRREERIGQLLQELKRSDKLHLKDAAALLGVSEMTIRRDLNN
                     HSAPVVLLGGYIVLEPRSASHYLLSDQKSRLVEEKRRAAKLAATMVEPDQTLFFDCGT
                     TTPWIIEAIDNEIPFTAVCYSLNTFLALKEKPHCRAFLCGGEFHASNAIFKPIDFQQT
                     LNNFCPDIAFYSAAGVHVSKGATCFNLEELPVKHWAMSMAQKHVLVVDHSKFGKVRPA
                     RMGDLKRFDIVVSDCCPEDEYVKYAQTQRIKLIY"
     gene            complement(35516..36718)
                     /gene="dacC"
                     /locus_tag="PN977_001332"
     CDS             complement(35516..36718)
                     /gene="dacC"
                     /locus_tag="PN977_001332"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HDI6041384.1"
                     /translation="MTQYSSLLRGLAAGSAFLFLFAPTAFAAEQTVEAPSVDARAWIL
                     MDYASGKVLAEGNADEKLDPASLTKIMTSYVVGQALKADKIKLTDMVTVGKDAWATGN
                     PALRGSSVMFLKPGDQVSVADLNKGVIIQSGNDACIALADYVAGSQESFIGLMNGYAK
                     KLGLTNTTFQTVHGLDAPGQFSTARDMALLGKALIHDVPEEYAIHKEKEFTFNKIRQP
                     NRNRLLWSSNLNVDGMKTGTTAGAGYNLVASATQGDMRLISVVLGAKTDRIRFNESEK
                     LLTWGFRFFETVTPIKPDATFVTQRVWFGDKSEVNLGAGEAGSVTIPRGQLKNLKASY
                     TLTEPQLTAPLKKGQVVGTIDFQLNGKSIEQRPLIVMENVEEGGFFGRMWDFVMMKFH
                     QWFGSWFS"
     gene            36965..37591
                     /gene="gstB"
                     /locus_tag="PN977_001333"
     CDS             36965..37591
                     /gene="gstB"
                     /locus_tag="PN977_001333"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308945.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase GstB"
                     /protein_id="HDI6041385.1"
                     /translation="MITLWGRNNSTNVKKVLLTLEELELPYEQILAGREFGINHDADF
                     LAMNPNGLVPLLRDDESDLILWESNAIVRYLAAQYGQKRLWIDSPARRAEAEKWMDWA
                     NQTLSNAHRGILMGLVRTPPEERDQAAIDASCKECDALFALLDAELAKVKWFSGDEFG
                     VGDIAIAPFIYNLFNVGLTWTPRPNLQRWYQQLTERPAVRKVVMIPVS"
     gene            complement(37588..38703)
                     /gene="yliI"
                     /locus_tag="PN977_001334"
     CDS             complement(37588..38703)
                     /gene="yliI"
                     /locus_tag="PN977_001334"
                     /EC_number="1.1.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose sugar dehydrogenase YliI"
                     /protein_id="HDI6041386.1"
                     /translation="MHRQSFFLVPLICLSSALWAAPATVNVEVLQDKLDHPWALAFLP
                     DNHGMLITLRGGELRHWQAGKGLSAPLSGVPEVWAHGQGGLLDVVLAPDFAQSRRIWL
                     SYSEVGDDGKAGTAVGYGRLSDDLSKVTDFRTVFRQMPKLSTGNHFGGRLVFDGKGYL
                     FIALGENNQRPTAQDLDKLQGKLVRLTDQGEIPDDNPFIKESGARAEIWSYGIRNPQG
                     MAMNPWSNALWLNEHGPRGGDEINIPQKGKNYGWPLATWGINYSGFKIPEAKGEIVAG
                     TEQPVFYWKDSPAVSGMAFYNSDKFPQWQQKLFIGALKDKDVIVMSVNGDKVTEDGRI
                     LTDRGQRIRDVRTGPDGYLYVLTDESSGELLKVSPRN"
     gene            complement(38814..39197)
                     /gene="bssR"
                     /locus_tag="PN977_001335"
     CDS             complement(38814..39197)
                     /gene="bssR"
                     /locus_tag="PN977_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssR"
                     /protein_id="HDI6041387.1"
                     /translation="MFVDRQRIDLLNRLIDARVDLAAYVQLRKAKGYMSVSESNHLRD
                     NFFKLNRELHDKSLRLNLHLDQEEWSALHHAEEALATAAVCLMSGHHDCPTVITVNAD
                     KLENCLMSLTLSIQSLQKHAMLEKA"
     gene            39410..40735
                     /gene="rimO"
                     /locus_tag="PN977_001336"
     CDS             39410..40735
                     /gene="rimO"
                     /locus_tag="PN977_001336"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HDI6041388.1"
                     /translation="MSKVTPQPKIGFVSLGCPKNLVDSERILTELRTEGYDVVPSYDD
                     ADMVIVNTCGFIDSAVQESLEAIGEALNENGKVIVTGCLGAKEDQIREVHPKVLEITG
                     PHSYEQVLEHVHHYVPKPKHNPFLSLVPEQGVKLTPRHYAYLKISEGCNHRCTFCIIP
                     SMRGDLVSRPIGEVLSEAKRLVDAGVKEILVISQDTSAYGVDVKHRTGFHNGEPVKTS
                     MVSLCEQLSKLGIWTRLHYVYPYPHVDDVIPLMAEGKILPYLDIPLQHASPRILKLMK
                     RPGSVDRQLARIKQWREICPELTLRSTFIVGFPGETEEDFQMLLDFLKEARLDRVGCF
                     KYSPVEGADANALPDQVPEEVKEERWNRFMQLQQQISAERLQEKVGREILVIIDEVDE
                     EGAIGRSMADAPEIDGAVYLNGETNVKPGDILRVKVEHADEYDLWGSRV"
     gene            40961..41347
                     /locus_tag="PN977_001337"
     CDS             40961..41347
                     /locus_tag="PN977_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HDI6041389.1"
                     /translation="MGVYKARRFSQSTKKLGIHDKVLMAAAEEVMQGIWEADLGSGVI
                     KKRLPLQQGKSGGARTIIFFKSANHVFFYDGWSKSGLSSKGTKEIEDDELAAYKKMAN
                     AFLAFSNKQIEDLIETGFLIEVKNER"
     gene            41337..41666
                     /locus_tag="PN977_001338"
     CDS             41337..41666
                     /locus_tag="PN977_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
                     /protein_id="HDI6041390.1"
                     /translation="MSDKLQMMLEDAKELNALGLMSDSEVDAIAVMVKAREVSARIAK
                     VKSMTGEEIKAIRTRYGMSQSVLAHTMGMSKESVSKWERNEIKPSGPALRILNTLAIK
                     GPEVFAK"
     gene            complement(41736..43064)
                     /locus_tag="PN977_001339"
     CDS             complement(41736..43064)
                     /locus_tag="PN977_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HDI6041391.1"
                     /translation="MSRINKFVLTVSLLIFIMISAVACGIYTQMVKERVYGLKQSVID
                     TAFAVANIAEYRRSVAIDLINTLNPTEEQLLVGLRTAYADSLSPSYLYDVGPYLISSD
                     ECIQVKEFEKNYCADIMQVVKYRHVKNTGFISFDGKTFVYYLYPVTHNRSLIFLLGLE
                     RFSLLSKSLAMDSENLMFSLFKNGKPVTGDEYNAKNAIFTVSEAMEHFAYLPTGLYVF
                     AYKKDVYLRVCTLIIFFAALVAVISGASCLYLVRRVINRGVVEKEAIINNHFERVLDG
                     GLFFSAADVKKLYSLYNSAFLDDLTKAMGRKSFDEDLKALPEKGGYLCLFDVDKFKNI
                     NDTFGHLLGDEVLMKVVKILKSQIPVDKGKVYRFGGDEFAVIYTGGTLEELLSILKEI
                     VHFQVGSINLSTSIGVAHSNECPTVERLKMLADERLYKSKKNGRAQISWQ"
     gene            complement(43072..>44038)
                     /locus_tag="PN977_001340"
     CDS             complement(43072..>44038)
                     /locus_tag="PN977_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HDI6041392.1"
                     /translation="TVNDFDSSFGYRNLRIHKSAICEPLQGENAWSYAEKLKLAISSI
                     RNHMFSEFIFCDDAKLNEIEENIWIARNIRHAMEIGELFLVYQPIVDINTRAILGAEA
                     LCRWVSAERGIISPLKFITIAEDIGFINELGYQIIKTAMGEFRHFSQRAVLKDGFLLH
                     INVSPWQLNEPHFHERFTTIMEENGLKVNSLCVEITETVIERINEHFYLNIEQLRKQG
                     VRISIDDFGTGLSNLKRFYEINPDSIKVDSQFTGDIFGTAGKIVRIIFDLARYNRIPV
                     IAEGVESEDVARELIKLGCVQAQGYLYQKPMPFSAWDKSGKLVKE"
BASE COUNT        11049 a        11261 c        10739 g        10989 t
ORIGIN      
        1 caggacgtat tgcaacgatc cagtctggta ctttgatacc gtaaaatctg gagcgtccat
       61 ctcattatcc ctgtaaaacg cgccctcagc ccacatccat gtgggctgcc ccggccttcg
      121 gggaacgctt cagcgatttt gacgccacca acaccggtgc ggtttgtaat taaagcgcaa
      181 gaacatcttt aaatgaaaac gaatactcta ctgctcctta tgcataacat ttccttttaa
      241 ttgcaacttt actcgtcctt ccgctttcag tagtgtgtca tcgcgcattt cgacttcatc
      301 tttgcaagga ctgggttttc atgttttctg ggctgttaat cattctggtt cccctgattg
      361 tgggttacct cattccgctt cgccaaaaag ctgcattaag agttattaat cagctattaa
      421 gctggatggt ttaccttatt ctctttttta tgggtatcag tctggcgttt ctcgataacc
      481 tcgccagtaa cctgttggcg attctgcatt attctgctgt cagtattacc gttattttac
      541 tgtgtaatat tgccgccctg atgtggctgg agcgcggcct gccatggcgc aaccaccatc
      601 atcaagaaaa actcccgtcg cgtattgcga tggcgctgga gtcgctaaaa ctgtgcggcg
      661 tagtagtgat tggttttgcc attggcctga gtggactggc tttcttacaa catgcgaccg
      721 aagccagtga atacacctta attctgctac ttttcctcgt cggtattcag ttgcgcaata
      781 atggcatgac cttaaagcag attgtcctta atcgccgggg aatgattgtc gccgtggtgg
      841 tggttgccag ttcattaatt ggtggtttaa ttaacgcctt tattcttgat cttcccatca
      901 ataccgcgct ggcaatggcc tccggtttcg gctggtattc tctttccggt attttattga
      961 ccgaatcttt tggtccggta atcgggagcg cggcgttttt taatgatctg gcccgtgagc
     1021 tgattgctat tatgttgatc cctgggctga ttcgccgtag ccgctcgact gcactgggct
     1081 tatgtggtgc gacgtcgatg gatttcaccc tgcccgttct tcaacgtact ggcgggctgg
     1141 atatggtccc ggcggcgatt gttcacggtt ttattcttag cctgttagtg ccgatcctca
     1201 tcgccttttt ctctgcataa tacctctctg gcggtagatc cctgccgcca aaattgcgct
     1261 aaatcaatct cccctaaagt tgcattaaaa atctctttta tccccgcgtt aagcgtctta
     1321 accttaaaca tgtatattaa atataacttt aaaaggtgtg atcatgtttt gtgtgcaatg
     1381 tgaacaaact atccgtactc cggcaggaaa cggctgctca tacgcgcagg ggatgtgtgg
     1441 taaaacggcg gaaacctctg accttcagga tttactcatc gcggcgctgc aagggctttc
     1501 ggcctgggcg gtaaaagcgc gtgaatacgg catcatcaac cacgatgtag acagctttgc
     1561 accacgtgcc tttttctcaa ccctgaccaa cgttaacttc gattccccgc gtattgtcgg
     1621 ctacgctcgt gaagcgattg ccctgcgcga ggcgctgaaa gcacaatgcc tggctgtaga
     1681 agccaacgcc cgcgtcgata acccgatggc tgacctgcaa ctggtgagcg acgatctcgg
     1741 cgagctgcaa cgtcaggcag cagaatttac ccctaacaaa gataaagcgg cgattggcga
     1801 aaacattctc ggcctgcgtc tgctgtgcct gtatggcctg aaaggtgcgg cggcctatat
     1861 ggaacacgcg catgttctcg gtcaatacga caacgatatt tatgcccagt accataaaat
     1921 catggcgtgg ctggggacct ggcctgccga tatgaacgcg cttcttgagt gttcaatgga
     1981 aatcggccag atgaacttca aagtgatgag cattctggat gcaggcgaaa ccagtaaata
     2041 cggtcacccg acgccaaccc aggtcaacgt caaagcgacg gcaggtaaat gcattctgat
     2101 ttccggtcac gatctcaaag atctctacaa cctgctggaa cagaccgaag gcacgggcgt
     2161 taatgtctac acccacggtg aaatgctgcc tgcgcatggc tacccggagc tgcgtaaatt
     2221 caagcatctg gtcggtaact acggcagcgg ctggcagaat cagcaagtgg agttcgctcg
     2281 tttccctggc cccatcgtga tgacctcgaa ctgcatcatc gacccaaccg taggcgctta
     2341 tgacgatcgt atctggaccc gcagcatcgt tggctggccg ggtgtgcgtc atctggatgg
     2401 cgaagatttc tctgcggtca ttgcccaggc gcaacaaatg gcaggcttcc cgtacagcga
     2461 aattccgcac ctgatcaccg tgggctttgg ccgccagacg ctgcttggcg cagcggatac
     2521 gctgattgat ctggtgagcc gtgaaaaact gcgtcatatc ttcctgcttg gtggctgtga
     2581 cggcgcacgc ggcgaacgtc actacttcac cgatttcgcc accagcgtgc cggatgactg
     2641 cctgatcctg accctcgcct gtggtaaata tcgctttaat aaacttgagt ttggcgatat
     2701 cgaaggtctg ccgcgtctgg tagatgcagg tcaatgtaac gatgcttact cagcgattat
     2761 tctggctgtc actctggcag agaaactggg ctgcggagtc aacgatctgc cgctgtcgct
     2821 ggtgctatcc tggtttgaac agaaagcgat tgtcattctg ctgacgctgc tttctctggg
     2881 tgtgaaaaat atcgtcaccg gcccgactgc acctggtttc ctgacaccag acctgctggc
     2941 agtgctgaac gagaaattcg gcctgcgttc tatcaccact gttgaagaag acatgaagca
     3001 actgttgagc gcgtaaggag tttgtcgatg acgatgccaa cgaatcaatg cccgtggcgg
     3061 atgcaggttc atcacattac gcaagaaacg ccggatgtgt ggacgatttc cctgatttgc
     3121 cacgattact acccatatcg cgccgggcaa tatgcactgg tcagcgtgcg taactcagcg
     3181 gaaacgctgc gtgcttacac catttcctcc acgccaggcg tgagtgaata catcaccctg
     3241 actgtgcggc ggattgatga cggtgtcggc tcccggtggc tgacgcgcga tgtaaaacgc
     3301 ggtgattatc tctggctttc ggacgcgatg ggggaattta cctgcgacga taaagcagaa
     3361 gataaattcc tgttgctggc ggcaggctgc ggcgttacgc cgattatgtc gatgcgtcgc
     3421 tggctggcga agaaccgtcc acaggccgat gtgcaggtga tctacaacgt gcgtacgccg
     3481 caggatgtga ttttcgccga tgagtggcgt aactatccgg taacgctggt ggcggaaaat
     3541 aacgttaccg aaggctttat cgctggtcgt ctcactcgcg aactgctggc aggtgtccct
     3601 gatttagcct cacgtaccgt gatgacctgt ggccctgctc catatatgga ttgggtagag
     3661 caggaagtga aagcgctcgg cgtgacgcgt ttctttaaag agaaattctt caccccagta
     3721 gcggaagcgg cgaccagcgg tctgaaattc accaaactgc aaccggcacg agaattttac
     3781 gccccggttg gcaccacgct actggaggcg ctggaaagca ataacgttcc ggttgtcgcc
     3841 gcctgccgcg caggtgtttg cggctgctgt aagacgaaag tggtttccgg tgaatatacg
     3901 gtgagcagca caatgacgct gaccgacgcc gaaatcgctg aaggttacgt actggcctgc
     3961 tcctgccatc cgcaggggga tttggttctc gcataatcgc ctgatgcccg atgatattcc
     4021 tttcatcggg ctatttaacc gtaagtgcct cctttttctc ccatcccttc cccctccgtc
     4081 agatgaacta aacttgttac cgttatcaca ttcaggagat ggagaaccat gaaacaaacg
     4141 gttgcagcat atatcgccaa aacactcgaa tcggcaggcg tgaaacgcat ctggggagtc
     4201 acgggcgact ctctgaacgg tcttagcgac agtcttaatc gcatgggcac catcgagtgg
     4261 atgtccaccc ggcatgaaga agtggcggcg tttgccgctg gcgctgaagc acaacttagc
     4321 ggagaactgg cggtctgtgc cggatcgtgc ggtcccggta acctgcactt aatcaacggc
     4381 ctgttcgatt gccaccgcaa tcacgttccg gtactggcga ttgccgctca tattccctcc
     4441 agcgaaattg gcagcggcta tttccaggaa acccacccac aagagttatt ccgcgaatgt
     4501 agtcactatt gcgagctggt ttccagcccg gagcagatcc cacaagtgct ggcaattgct
     4561 atgcgcaaag cagtgctaaa ccgtggcgtt tccgttgtcg tgttaccggg cgacgtggcg
     4621 ttaaaacctg caccagaagg ggcaactacc cactggtatc atgcgccaca gccagtcgtg
     4681 acgccggaag aagaagagtt acgcaaactg gcgcaactgc tgcgttactc cagcaatatc
     4741 gccctgatgt gcggcagtgg ctgcgcgggg gcgcataaag agttagttga gtttgccggg
     4801 aaaattaaag cgcctattgt tcatgccctg cgcggtaaag agcatgtcga atacgataat
     4861 ccgtatgatg tcggaatgac gggattaatc ggcttctcgt caggtttcca taccatgatg
     4921 aatgccgata cgttagtgct gctcggcacg caatttccct accgcgcgtt ctacccgacc
     4981 gatgccaaaa ttattcagat tgatatcaac ccagccagca tcggcgcgca tagcaaggta
     5041 gatatggcgc tggtcggcga tatcaaatca accctgcggg cattgctgcc actggtggaa
     5101 gaaaaaaccg atcgcaagtt tctggataaa gcgctggaag attaccgcga cgcccgcaaa
     5161 gggctggatg atttagctaa accgagcgag aaagccattc acccgcaata tctggcgcag
     5221 caaattagtc attttgccgc cgatgacgcc atctttacct gtgacgttgg tacgccaacg
     5281 gtgtgggcgg cacgttatct gaaaatgaac ggcaagcgtc gtctgttagg ttcgtttaac
     5341 cacggttcga tggctaacgc catgccgcag gcgctgggtg cgcaggcaac cgagccggaa
     5401 cgtcaggtgg tcgccatgtg cggcgatggc ggtttcagta tgttgatggg cgatttcctc
     5461 tcagtaatgc agatgaaatt gccagtgaaa attatcgtct ttaataacag cgtgctgggc
     5521 tttgtggcga tggagatgaa agccggaggc tacctgacag acggtactga gctgcacgac
     5581 accaactttg cccgcattgc cgaagcgtgc ggaattacgg gtattcgcgt ggaaaaagcc
     5641 tctgaagtcg atgaagccct gcaacgcgcc ttttccatcg acggtccggt gttggtggat
     5701 gtggtagtag ccaaagaaga gttagccatt ccaccgcaga tcaaacttga acaggccaaa
     5761 ggatttagct tgtatatgtt gcgcgcaatc atcagcggac gcggtgatga agtgatcgaa
     5821 ctggcgaaaa cgaactggct aaggtaaaaa gggtggcatt tcccgtcata ataaggacat
     5881 gccatgattg atttacgcag tgataccgtt acccgaccga gccgcgccat gctcgaagca
     5941 atgatggccg ccccggttgg ggacgacgtt tacggagacg accctaccgt taatgctctg
     6001 caggactatg cggcagagct ttccggtaaa gaagccgcca tttttctgcc gaccggcacc
     6061 caggctaacc tggtcgctct gctcagtcac tgcgaacgcg gcgaagagta tattgtcggt
     6121 caggccgcac acaactatct gtttgaagcc ggtggcgcag cggtgctggg cagtattcaa
     6181 ccgcaaccca tcgacgcggc tgccgacggc acgctaccgc tggataaagt ggcgatgaaa
     6241 atcaaacccg acgatatcca tttcgcccgc accaaattac tcagtctgga aaacactcac
     6301 aacggcaaag tgctgccgcg tgaatacctc aaagaagcat gggaatttac ccgcgagcgc
     6361 aatctggcgc tgcatgtgga cggtgcgcgc atctttaatg ctgtggtggc ttacggctgc
     6421 gaactgaaag agatcacaca atattgtgat tcgttcacca tttgcctgtc gaaaggtctt
     6481 gggacgccag tcggttcatt actcgtcggt aatcgtgatt acattaaacg tgccattcgc
     6541 tggcggaaaa tgacaggtgg cgggatgcgc cagtccggca ttctggctgc cgccggaata
     6601 tatgccctga aaaataacgt tgcgcgcttg caggaagacc acgacaacgc tgcctggatg
     6661 gcggagcagc tgcgtgaagc aggcgcggat gtgatgcgtc aggacaccaa tatgctgttt
     6721 gttcgcgtcg gggaagaaaa tgctgccgcg ttaggcgaat acatgaaagc gagaaacgtg
     6781 ctgattaacg cctcgccgat tgtccgcctg gtgacgcatc ttgacgtctc gcgcgctcag
     6841 ctggcggaag tcgccgccca ctggcgcgca ttcctggcgc gttaaggagg gagacgtgcc
     6901 gcaacgcatt ttagttctcg gtgccagtgg ctacattggt cagcatctgg tgcgaacact
     6961 cagccagcag gggcatcaga tcctggcggc ggcacgtcat gtcgacaggc ttgcaaagct
     7021 gcaactggca aatgtcagtt gccataaagt cgatctcagc tggccggata accttccggc
     7081 cctgttgcag gatatcgata cggtctattt tctggtgcac agcatgggcg aaggcggcga
     7141 ttttatcgct caggagcgcc aggtggctct caacgtccgc gatgcgctac gtgaagtccc
     7201 agttaagcaa ttaatctttc tcagttcgtt gcaggccccg ccacatgagc agtcggacca
     7261 tctgcgcgcc cgtcaggcta cggcggatat tcttcgtgaa gcgggtgtcc cggtgaccga
     7321 attgcgtgcc ggaataatcg ttggtgcagg ttcagcggcg ttcgaagtca tgcgcgatat
     7381 ggtctacaac ctgccggtgt taacgccgcc acgctgggtt cgttcacgca ccacgcccat
     7441 cgcgctggaa aacttgctgc actatctggt ggcgctgtta gaccatccag ccagcgaaca
     7501 tcgcatcttc gaagccgccg gaccagaagt gctcagttat cagcaacagt ttgaacattt
     7561 tatggcggtg agcggtaagc gccgctggtt gatccccatc cccttcccca cccgctggat
     7621 ttcggtgtgg tttctcaatg tgattacttc cgtaccgccc actaccgcca aagcgttgat
     7681 tcaggggctg aaacacgatc tgctggcaga tgacaccgcg ttacgtgcac tcatcccaca
     7741 acggctgatt gctttcgatg acgcggtacg tcgcaccctg aaagaggaag aaaagctggt
     7801 caactccagc gactggggat acgacgctca ggcctttgcc cgctggcgac cggagtacgg
     7861 ttatttcgcc aaacaggcgg ggtttaccgt taaaacgtcc gccagccttg cggctttatg
     7921 gcaggtggtg aatcaaatcg gcggtagaga gcgttatttc tttggtaata ttttgtggca
     7981 gacccgggcg ctgatggacc gcgcgatcgg tcataagctg gcgaaaggcc gcccggagcg
     8041 tgaatatttg caaactggcg atgcggtaga cagctggaaa gtgattatcg tcgagccgga
     8101 aaaacaactt gcgttgttat ttggcatgaa agcgccgggg ctgggacgac tgtgttttac
     8161 cctggaagat aaaggcgact atcgtactat cgatgttcgc gcattctggc atccgcacgg
     8221 gatgccgggg ctgttttact ggttactgat gatccccgcg catctgttta tttttcgcgg
     8281 aatggcaaaa cgaatcgcca gactggcaga acaaagcaca gattaatcga caaaacctgc
     8341 gttattcttt catgtttccc attgcatcat aggggaaatc acggaagaat gcgcacgtca
     8401 ttttctcaac aacagtggat tgatatgaag gtactggtta ccggcgccac cagcggctta
     8461 ggtcgaaacg cggtagagtt tttatgccag aaaggcatca gcgtgcgtgc gaccggtcgc
     8521 aacgaggcaa tgggtaaatt gctggaaaaa atgggcgcag agtttgttcc ggcggatctg
     8581 accgagctgg tctcatcaca agctaaagtg atgctcgcag gcattgatac gctgtggcac
     8641 tgctccagct ttacctcgcc ctgggggaca caacaggctt tcgatctggc taacgttcgc
     8701 gccactcgcc gcctgggtga atgggctgtc gcctggggtg tgcgtaactt tattcatatc
     8761 tcttccccct ccctgtactt cgattatcac caccatcgcg atattaaaga agattttcgc
     8821 cctcaccgct tcgccaacga gtttgcccgt agcaaagcag ccagcgaaga agtgatcaat
     8881 atgctttcgc aggcgaatcc acaaacgcgc tttactattc tgcgcccaca aagtctgttc
     8941 ggaccgcacg ataaagtctt tatcccccgt ctggcgcata tgatgcacca ctacggcagc
     9001 attctgttac cgcacggcgg cagtgcgctg gtcgatatga cctactatga aaatgccgtg
     9061 cacgccatgt ggctggcaag ccaggaagcc tgcgataagc taccttccgg gcgtgtgtac
     9121 aacatcacca acggcgagca tcgcacactg cgcagcatcg tgcagaagct gatcgatgag
     9181 ttgaatattg actgtcgtat tcgttcggtt ccttacccga tgctggatat gatcgcccgc
     9241 agcatggagc gtttaggccg caagtcagca aaagagccgc cgctgaccca ctacggcgtc
     9301 tccaagttga attttgactt tacgctggat attacgcgtg cgcaggaaga gttaggttat
     9361 caaccagtcc tcaccctgga tgaaggtatc gagaaaactg ccgcctggct acgcgaccac
     9421 ggaaaactgc cgcgctaatc ctgcccgtat ttctccagca acgcttcggc aatcgcctga
     9481 gtttctgcat ccgcttcgcc gttgtataac gtcgggcgga aatgcatctg gaacgccata
     9541 atcacgcgcc gctgctcgcg cggtgtcata tcaggtttaa cgtcataacc gtagcgcgcc
     9601 aaaagctcca gcaatgacgc ggtatctacc ggtgtgtgcg gcgcgcgccc ggcaaggtaa
     9661 aagttcaccc gctgcgcatc cggccaggca ccaatcccct gctgcgccag ttgctgccag
     9721 ggaaataatg gccccggatc gtctttgcgc tgcggggcga tatccgcatg tgccactacg
     9781 ttttccggct tgatgtgata acgggcaata atatctttcg ccagtggaat aagcgcctga
     9841 atctgtgccg gttcaaacgg ggcaaaatat ttcactccgg ctgatttctg ccagccacgg
     9901 ttttccagct caatgccaat agaggtgtcg ttaaggcgcg ttgccccgcg ccaggcgcta
     9961 atccccgcat gccaggccag ttcctgttcc ggcaccagtt gccagatgcg cggtttaccg
    10021 ttgtatcgcg gtggcaccgc agggaccaga taatgcgagc tgacctgctt gtcggtcagt
    10081 gtcgccagcg agctatcaaa atcatctgcg gtgtagtgga taaccatcac tttaatgcgc
    10141 ggatacgccg cctgcgcctg gcgtcgggta tcaagctgat atccctcttt ctcgacaatg
    10201 cccttttcgc ctgcacaccc tgccagtaac agagcggcgg cgaccagcca gaagactctt
    10261 ctcatcgacg cgttttcacc gccgtaccgc tgacgctaac catcagcata ctgccgtttt
    10321 gcccgaccgt ttcatagtcg atatcaatac cgactacggc atcggccccc agcgcccggg
    10381 cctgatcgcc gagctcctca aaggcgatct cccgcgcttt acgcaattct ttttcataag
    10441 ccccggagcg tccgccaacg atatcgcgga taccggcaaa gaaatcacgg aaaatattgg
    10501 cacctaaaat cgcttcgccg gtcaccacac cgcaatattc gacgatggtc tggccttcca
    10561 gagttggggt tgtagaaaat tgcatgatcg atctccttgt ttgttaagcc ttcgatctct
    10621 aaagcattat cggactgata tgccatgttg ggaagaaggt tttgatcagt ttaggaaatc
    10681 atctcattgt gtcaaaaaca ggtacataag cctgtacaat tatctgtttc gtaaccggat
    10741 attggtttca ttatggagca ccgcttacct gccctattat gttgtgccgg caacattaat
    10801 gagtgggaag agccaactta tattgtaaat gattcctaaa gtcttcccca tcttgcgcct
    10861 gaggtatata cctctggatt gctttttggt cagccgccgc aaaacaagaa agtaataaga
    10921 tctcaatgct tttgtcatta atataagcga agatcattct tgcaaattcg tatataaacg
    10981 cccgatcctg agcgcttaat agtttaatac aatatttatt tttaagaaaa taaagtattt
    11041 caaggaaagc atccgcagca accttcacgt tattaccggc ggtcatatta tatatatctc
    11101 ttgcacagat agagtacaga gatagtaaac agccatacaa ataatcattt attaattctt
    11161 ttgtccattt tactggcaac catggtttta tcttgacacc atgcatacta cagtcatgaa
    11221 aaagacattt actgaaatta actcttatca tatcaacatt gaaaaaatgg cagttaataa
    11281 attttaaaga atgagtattt acgttgttaa ttttaacaga tctaaacgaa atatttttga
    11341 atacactgtt tattacattg ctatttgtga ttttaacatc acctaaactg ccgttttcaa
    11401 aacaacattt tttcaatatg ctttcattca caatagtgtg tgataaatag agattattta
    11461 tgttttgctt tattaatgcc acgtcattaa aatgactata tttaataaag caaccatcat
    11521 tgagtagtgt tttaagattg actaaattag ttgcatcttc acgtaccatc atggcaactg
    11581 gaacaggcaa cgacataaga acttcttcat tcaccatcaa gtgaatcatg cattgcgttc
    11641 cctttgtcat atccacactt acgctacaaa tatcgttgta atgcgaagag tgcatttcag
    11701 gaaacaaatt aaacacggta tcttgaaagg cactaaatcg tgttaataaa tcctgataat
    11761 gaacaggttt tgtttcaaaa ttatcatcag caaatccaag tactattgca tgctcgatat
    11821 ttaacgcagt ttcaaatgat tcttcttctg taaaatcatt atttaaaacc tcacctgacg
    11881 tattttcact gtcataaaaa gtactcatca atgcgatatt gtttgttaca gcgtcttttg
    11941 cgatattctc actaattgca tatcgcctgc tataatcata aaaatgaata aacgcatcct
    12001 ttataaagga tatatattgc tctttgttaa agtcaatacg actgtataat ttttcaaaaa
    12061 tcacattcgc aatcttacta cccatagtat gcctgtataa taatggatgc gattcaatcg
    12121 taaaatgctt gtctaattga tgttgtaatt tattctcata gtccagtggg aaaagaaatt
    12181 tttgtctttc aagtttccca ttcgatacat tacgtattcc aatggtaatt ctttcattac
    12241 ccacttttat ttcaacttcg ttatcttctg atttgccaag atatttttta acatcaatct
    12301 cgaaagcatt actaaaaaat atattaaatt gataattcgc tgatgtaata ttttccgcta
    12361 gcaactcatc attttttatg agatcactat cagcatttat tgctaatatc agatgcaagt
    12421 agatggtgtc ccatgtagac aactcaccct tacgactgga atttaatatc gcgaaaaatt
    12481 ccctcagtga atcgaggaaa ttatcacttt gattcagttg tgtgcctgga aatgatgatg
    12541 cttgatcaga tatattgttt atcatattaa aaatcaccca agggaaaaga tattccactg
    12601 aactcctcgt gctttattat tataaaagca caacgcattt atactcatat taattaacga
    12661 aacactgata cagtttgatt tattcttcgc tcaaactcat tagcttaaat tctttaccca
    12721 caactgtagc atatataata ttatcaaaag tcaaagctgg gactatcaaa tttcgctcat
    12781 tttcaaaaat acaatgtaat tttagcaatc atcttaattg caggatctct tattgcatgt
    12841 gaaaaattct gtaatgatta gacaataacc gcgttcattt atcacgcgtt ataaatgtga
    12901 aactggcttc ttgcagatgc tcttaacctc tacaggagga tttatcccgc catttcagaa
    12961 ctatcatatt taccttaaat gatcatttca gccatataac acgctcaacc tcacaaatgg
    13021 tttacactgt ctattgtttg ggtaatttaa gtaagtcagc tacgagcaac cttgagtggg
    13081 tagccagcaa tggactcaaa cccaatcagg tgttcagatt gtttcagaat tgtattaaaa
    13141 acaatgtatt aaataatctg ataacgtttg gtcgtagtgt gacgcagaca gcaagctaat
    13201 aaggaaatga ttatgcgcta ccgttcacta tctctactta ttccatgtgc actgctgctg
    13261 agtgcctgta ccaccgtcac gccagcttat aaagataacg gcccccgcac cggttcctgt
    13321 gcccagggtg gccctgacag cgtggctcaa caattctatg actaccgcat tcaacatcgt
    13381 aacaatgaca ttaccgccct gcgcccctat ttgagtgaca aactggcaac cctacttagc
    13441 gatgccagcc gcgataacag tcatcgtgaa ttgctaagca gcgatccgtt ctccagtcga
    13501 accaccttgc cagacagcgc ccacgttgcc agtgcatcga ctatccctaa tcgtgatgcc
    13561 cgtaacattc cattgcgcgt ggatctcaaa cagggtgacc agggctggca ggatgaagtg
    13621 ttaatgattc aggaaggcca gtgttgggtc atcgacgatg tgcgctactt aggcggtagc
    13681 gttcatgcca cagcgggtac gctccgtcag tccatcgaga atcgctaacg ccttgatcaa
    13741 ccaataaact ccgtaaattg tccggcattt cgtgaggaat gccgacattt atgctcgccg
    13801 accaccgccc ccgttatttt gtgctatgtt tattgaataa tgcgctttgc ttttaacttt
    13861 taaagcagaa atattgcata attattctgt caaaggtact atcttcgacc tcaatagcta
    13921 tcagactgcc agtatacgag tgtcaatgag tattcaatta aacggcatta attgcttcta
    13981 cggcgcgcat caggcgctgt tcgatatcac gctggattgc ccacagggcg aaacgctggt
    14041 gttacttggc cccagcggcg cggggaaaag ctcgctgctg cgtgtactca atctgcttga
    14101 gatgccgcgc tccggtacgc tcaacattgc aggcaaccat ttcgatttca ccaaaacacc
    14161 ctctgacaaa gcgattcgcg atttgcgtcg taacgttggc atggtgtttc agcaatacaa
    14221 cctatggccg catctgaccg tgcagcaaaa cctgattgaa gcgccctgcc gtgtactggg
    14281 gttgagcaaa gatcaggcgc tggcccgtgc agaaaaactg ctggaacgtc tgcgtctcaa
    14341 accttatagc gatcgttacc cgctgcatct ttctggtggt cagcagcagc gtgttgctat
    14401 tgcccgtgcg ttgatgatgg aaccgcaggt actgctgttc gatgaaccga ccgccgcact
    14461 ggacccggaa attacggcac aaatcgtcag catcattcgt gagttggcag aaacgaatat
    14521 tacccaggtg atcgtcaccc acgaagttga agtggcgcgt aaaaccgcca gccgagtggt
    14581 gtatatggaa aacggtcata tcgtagaaca aggcgacgcg agctgcttta ccgagccgca
    14641 aaccgaagca tttaaaaact atctctctca ctaatattcg ggataacaac aatgaaaaaa
    14701 gttctgattg ccgcgttaat tgcaggtttt agtctttccg ccacagctgc cgaaactatt
    14761 cgttttgcta ccgaagcctc ctatcctccg tttgaatcga ttgatgcaaa caaccagatc
    14821 gttggttttg acgtcgacct ggcacaagcg ctgtgtaaag agattgatgc aacctgcact
    14881 ttctccaacc aggcatttga cagcctgatc ccaagcctga aattccgtcg cgtggaagcg
    14941 gtgatggccg ggatggatat caccccagaa cgtgaaaagc aggtgctgtt taccacccct
    15001 tactatgaca actctgccct gtttgtgggt cagcagggta aatacaccag tgttgatcag
    15061 ctgaaaggca aaaaagtcgg cgttcagaac gggacgacac accagaagtt cattatggat
    15121 aagcacccgg aaatcaccac cgttccgtat gacagctacc agaacgcaaa actggatctg
    15181 caaaacggtc gtatcgacag cgtattcggt gacaccgcag tggtcaccga gtggctgaaa
    15241 gacaacccga aactggcggc agtgggcgat aaagtgaccg ataaagatta cttcggtact
    15301 ggtctcggca tcgcggtacg tcagggcaac actgaactgc agcagaaact caacactgcg
    15361 ctggaaaaag tgaagaaaga tggcacttac gaaaccatct acaacaaatg gttccagaag
    15421 taattcctga tgaatgaatt ttttccttta gcaagcgccg ccgggatgac cgtcggcctt
    15481 gccgtttgtg cattgattgt cgggctggcg ctggcgatgt tctttgcggt atgggagtcg
    15541 gcaaaatggc gtcctgtcgc gtgggcaggt tcagcgctgg taaccattct gcgtggcctg
    15601 ccagaaattc tggtggtgct gtttatctat tttggctcct cgcagctgct gctgacgctt
    15661 tcggatggct tcactatcaa tcttgggttc gtgcaaatcc cggtgcagat ggacattgag
    15721 aacttcgacg ttagcccgtt cctttgtggt gtcatcgctc tgtcgctgct gtatgccgcc
    15781 tatgcctcgc aaacgctgcg cggcgcgttg aaagcggtgc cggtgggcca gtgggaatcc
    15841 ggacaggcgc tggggctgtc gaaatcggct atctttttcc gtctggtgat gccgcagatg
    15901 tggcgtcatg cgctgcctgg tctcggtaac cagtggctgg tgctgctgaa agataccgcg
    15961 ctggtcagtt tgataagcgt gaatgattta atgctacaaa ccaaaagcat tgctactcgt
    16021 acccaggaac catttacctg gtacattgtg gcggcggcga tttacctggt gatcaccctg
    16081 ctcagtcagt acattctcaa acgcattgac ctgcgcgcga cacgttttga gcggaggccc
    16141 agctaatgtt tgagtattta cccgaactga tgaaagggct gcacaccagc ctgacgctaa
    16201 ccgttgcctc gctgattgtg gcactgattc tggcattgat ttttaccatc atcctgacgc
    16261 tgaaaacgcc ggtgctagtg tggctggtac ggggctatat cacgctgttt accggtacgc
    16321 cgctgctggt gcagatcttc ctgatttatt acggaccggg ccagttcccg acgttgcagg
    16381 agtatccggc actgtggcat ttgttgtcag aaccgtggtt atgtgcgctg attgcgttgt
    16441 cgctgaacag tgcggcgtac accacgcagc tgttttacgg tgcgattcgc gcgatcccgg
    16501 aaggtcaatg gcagtcctgt agcgccctgg gaatgagcaa aaaagatacg ctggcgatcc
    16561 tgctgccgta cgcgtttaaa cgctcgctct cttcttattc caacgaagtg gtgctggtgt
    16621 tcaaaagtac ttctctggct tacaccatta cgctgatgga agtgatggga tacagccagt
    16681 tgttgtacgg acgcacctac gatgtcatgg tgttcggtgc ggcagggatt atttacctga
    16741 ttgttaacgg tctgctgacg ctgatgatgc gtctgatcga gcgtaaagca ctggcgtttg
    16801 agcgaaggaa ttaatttggc tgcgtgctga tgccggatgc ggcgtggacg ccttatccgg
    16861 cctacatagt gcataaattc ttataatgaa gacggacaac tcactaagct gtccgtcttt
    16921 ttttatttca tttaaattat ttaatcatat ttattgcata taaattcact tgatggcatt
    16981 gttatcccat gccgcagaca cggccaaaaa tcataagatt gacagacggg agttccatca
    17041 tgaaaaagtt agttcttgcc gctttacttt catcctgtgc attcggcgct tctgccgcag
    17101 agaaaatcaa ttttggcgtt tcagccacct atccaccctt tgaatctata ggtgctaata
    17161 atgagattgt cggctttgat atcgatctgg caaaagcctt gtgcaaacaa atgcaggcag
    17221 aatgtacttt tacgaatcac gcgttcgaca gcctgatccc gtcactgaaa ttcagaaaat
    17281 atgacgccgt aatctccggt atggatatca ccccggagcg tagcaaacag gtatcgttta
    17341 ccacgcccta ctatgaaaac tcagccgtcg tgattgccaa aaaaaatacc tacaaaacgt
    17401 ttgccgatct gaaaggcaaa cgtattggga tggaaaacgg caccacgcac cagaaatata
    17461 ttcaggatca gcacccggaa gtgaaaactg tctcctatga cagttatcag aatgccttta
    17521 tcgatctgaa aaatggccgt attgatggcg tatttggtga caccgcagtg gtaaacgaat
    17581 ggctgaaaac caatccgcaa ctgggcgttg cgactgaaaa agtgaccgat ccgcaatact
    17641 tcggcaccgg cctgggtatc gctgtacgtc cggataacaa agctctgctg gaaaaactga
    17701 ataatgcgct ggcagcaatt aaagctgacg gtacatatca gaaaatcaat gaccagtggt
    17761 tcccacagta agattcagaa agccgcacct gcggcttttt tattgcttca ccagcagcgt
    17821 cagcacttca tagtgcgcgg tgtgcgggaa catatcaaaa agctgtaccc gttcaatacg
    17881 gtaacctggc agttcgcgga tatctttcgc catggtttgg gcgttacagc tggagtatat
    17941 gataaaacgc ggtgccatcg ttgagagata atcacacagc ggtataccaa tgccgcggcg
    18001 cggcgggtta accagcacca gctccggcac ttccccctgg gcggtggcaa actgcgtaga
    18061 gtccagcgcc tgaaattgca aacgcgttaa gcccagttca gcggctgact gcttcgcaca
    18121 ggcaatggcc tctggtgcaa tttcgatccc ggttaactga atgtcaggcg tcgcgcagtg
    18181 taaaccaaag ccccccacgc cgcagaagag atcccacata tggtttaccg gtagttgccg
    18241 cacccagtcg cgcgcggtag cgtaaagctg gctggcgacc gccggattgg tctggaagaa
    18301 actttgcgga cggatccaca gcggcacatc gttaaaacgc tccgccagcg cctgttgttc
    18361 ggtcaggtag atctccgtct ccccttccat aatcgccata tgtaccggct gaatattgac
    18421 ggtaataact ttcagctgcg gtaattgttc ctgtaaccac ggcagcgcct tacgcagttg
    18481 cgccagtttg gtatcagaac gcagtacaaa acgcagcatc atgccgccat cgctctggct
    18541 ttcagtcagc agaatgtatt tcagttcacc acgtttacgc gccacgttgt aaggtgttaa
    18601 ccccgcacgg gcgatgaagg gttttagcgc cgcaaaaacg ggcgcaaatg aggctggata
    18661 aagcgggcag tcacaaaggt cttccggtgt accatctcga tgcagcatac cgagcagtgg
    18721 tttttcaaca ctaccactca ccaccatttt ggctttatta cgaaaccctt gttccgggcc
    18781 tgacaccggc gcacaccatt cctcaaccgg aaaatcggcg agcagatttt taagatcggc
    18841 ggttttagcg gagagttgct ctggaatcgg ctgcgttatc cactgacagg aacgacagcg
    18901 acccgcgtcg taaagtgcgc actgcataca ttgaccttca catcatcagg ggcgacgatt
    18961 atacacatta ttgcaactgg aagaaccgcc gactggtcga gggaacgaac agcagcatca
    19021 ggatgagcat atccggcagc ttctgcagta tcaggctatg gaagatttca cgttttgatt
    19081 caccgggaat gctgaacagc tccggataac cataccccag cgaagctgcc cacaagtaac
    19141 ttgcggccgt gatttgagtc aacagataca gccagcgcgc ccagcgacgc cctttcacca
    19201 gtgaaaacgc acaccagatc tcaataaaaa ccagcaccag actgcttaaa aagaccagcg
    19261 ttaaactcca ggtttgtacg ctgcgatgaa tgaactcgcc aataccgcgc acgcccagcg
    19321 tattgaaaat catcagcacg tcgaggccac ggatcataat aatggcgagc gccgctacct
    19381 gcaccagcgc aggcacattc aggcgagcat gagatgaaga tgttttctta aaaaatccca
    19441 acgtttcgtc ttccatgaaa acaatgccgc gacatgcgcg gcattatgta gccaggttgg
    19501 caaattttag tgtcttcagc cacgtcttgc acgctggata tcgcgtatcc gctgtttttc
    19561 tgcgcgcgcc atcagatacc aggcgataaa tccgacaatt ccgaccgcgc cgaggattaa
    19621 tgttgccagg gcgttgattt ccggattcac ccccatccgc acgctggaaa agaccagcat
    19681 cggtaacgtg gtggctcccg gcccggaaac aaagctggcg atcaccagat catcaagcga
    19741 caaagtaaaa gccagtaacc agccagaaat gatcgcgggc atgatcatcg gtagcgtaat
    19801 gacgaaaaac actttcagcg gcgtcgcacc gagatccatc gctgcttctt ctatcgagct
    19861 atccagttcc cgcagacgcg acgaaataac gactgccaca taagccgtac agaaagtgac
    19921 atgcgccagc cagatggtga gcataccgcg gtccgcaggc cagccaatgg catgagcaag
    19981 cgcgacgaat aacaacaaca gcgacaagcc tgtgatgaca tctggcatca ccagcggcgc
    20041 ggtgatcata aaggcaaagc catttgatcc gcgaaacctg ccaaagcgca ccagcaccac
    20101 cgccgcaata gtcccgagga tcgccgccgc cgtcgccgca caggccgcaa ttgtcaggct
    20161 taaaccaacc gcactcatca tcgcgtcatc gcgcaataat tcgccatacc agcgcgttga
    20221 ccagccggcc cacaccgtca ccagcttcga gctgttgaat gaatagatca ccagcatcag
    20281 cattggcgcg tagagaaagg tgaagcccag cagcaaaatc acaatccgcc agggcgaacg
    20341 aactaccggt aaattattca tccgtgttct cccacgcttt tttgctgatg cttgtggaac
    20401 cacattatcg gcacaattag cagcagcaac atgatgatcg ctaccgctga ggccaccggc
    20461 cagtcgcggt tgttaaagaa ctcctgccac agcacgcgcc cgatcatgat gctgtccggg
    20521 ccaccgagca gttcagggat cacaaactcg cccacagccg ggataaacac cagcatcgat
    20581 ccggcaataa tcccaccttt agtcagcggc acgatcacag taaagaacgt tttcagtggt
    20641 cgtgcaccga gatccagtgc tgcttccacc agcgaataat caattcgaat caacgcggta
    20701 taaatcggca gtaccataaa cggcacgtag gcgtaaacaa tgccgatata aacggccaga
    20761 ttggtatgta gaatggtcag cggttgatcg ataaccccca gccacagcag aaaattattc
    20821 agtacgccgt tattttttaa tattcccatc caggcataaa cgcgaatcag aaacgaggtc
    20881 cacgacggca ggatcaccag tagtaataag atattacggg tcgaaggctt actgtgcgcc
    20941 accgcccacg ccagcggata gccgatcagt aaacagcaaa tagtcgaaat cgccgccacc
    21001 tgtagcgact ggagataagc atcgaaatag agcggatcgt cagtcagttg cagaaaatta
    21061 ccgagattaa gagtgatgga aagttgcccg tcagcccact ccatcagttc ggtataaggt
    21121 ggaatagcgc gcgccatctc cgccaggctt attttaaaga caatcagaaa tggcagcaga
    21181 aacagcagga ttagccagat atacggcagc gcaatcacca gtttgcgccc atgcttcatt
    21241 tgcagctgcg acaaccacag cttaaatccg cccggcggtt tcgactgggc agcaggttca
    21301 agtgtactca tctttgctcc ttaaaccgtc agcaccacac agctgtccac ttcccagcac
    21361 aaacgtactt cgtcgcccca ggtcggtaac cctttacgat gacgatgggc gttttgtagc
    21421 tgggcgctaa tcatctgccc acttttcaga cgaacgtgat acaccgaaag atcgccgaga
    21481 taggcaatgt gtatcacctc ccccaccgcg aagttacaac cattggcggg cggttcctcg
    21541 caaagcatga ttttttccgg gcgaagcgcc acatgcaccg gcacgttatc gaccactgaa
    21601 gcatccgcat cgactttcag tgggtgcacc agtcccggcg aataaagcac cagaccatct
    21661 tcctgacgct ctttgagtac accttcaaag acattaaccg aaccgataaa ttcagcgcta
    21721 tagcgggtgg tcggatgctc atagatctct tccggttcgc caatctggac aaatttccca
    21781 cgattcataa tggcgatacg ccccgccatg gtcatcgcct cttcctgatc gtgagtaacc
    21841 atcacgcagg tcacaccgac gcgctccaga atatccacca cttcaagctg catccggtca
    21901 cgcagctttt tatccagcgc gcccatcggc tcatcgagca gtaatagttt cgggcgcttg
    21961 gcaaggcttc gggccagggc cacacgctgt cgctgaccac cggaaagctg atgcggtttg
    22021 cgtttggcga actcctgcat atgcaccagc ccgagcatct cattgacccg gctggcaatt
    22081 tccgctttcg gtagtttgtc ctgtttcagg ccaaaagcga tgttctgttc cacggtcata
    22141 tgcggaaaca gcgcgtaaga ctgaaacatc atattgatgg ggcgcaggta aggcggcacc
    22201 tgtgacaaat cgacgccatc gagcataatc tgcccggcag aaggttgttc gaaacctgcc
    22261 agcatacgca gcagcgtgga cttgccacag ccggatgcgc ccagcagcgc gaagatttcg
    22321 cctttataga tggtcaggct gacatcatcg accgcatgct gaccatcgta agatttagtc
    22381 agattacgga tttctaatag cggcgtcagc gccttacggg ttttcgcctg cgggcgaggg
    22441 atagcgtcat tcacggctgc tctccggcat tgaatgtgtg caagcgcacc ataatggtgc
    22501 gaattgccgg cgggtgtggt gcgcccgtcc ggcatctacg actgcggatt attttccgct
    22561 cttcactttg gtccacgcgc gggtgcgcac acggtctatt tttggatcct gcactttcag
    22621 agtgaacaac ttcgcacgaa cgtccgccgg aggataaatc cctggattat cacgcacttc
    22681 cgcgcttacc agcggcgtag cttctttgtt cgcgttggca tagaacacat ggtcggaaat
    22741 atgcgccacc acatccgggc gcagcaggta attcaggaac tgataggctt cgtctttatt
    22801 tttggcatcc gcaggcatgg cgaagacatc aaagaacgcc atcacccctt ctttcggaat
    22861 cgagaacgag acattaacgc cattcttcgc ttccttcgcg cggtttgacg cctgccagac
    22921 atcgcccgcc cagccaatag cgacgcagat atcgccgttt gccaggtcgt taatgtattg
    22981 agatgaatgg aaataacgaa tgttcgggcg cagctttaac agcagatcgg ttgccggtcc
    23041 ggtgtaatca tctgctttag tgctgttggg atctttaccg agataattca gcacggtagc
    23101 aaacacttct tccggtgcat caaggaaaga gacgccgcag cttttcagtt tttccagatt
    23161 ttcaggtttg aggatcaagt cccagctatc gacgggcgcg ttttcgccca gcaccgcttt
    23221 gactttatcg acgttatagc caatcccggt tgtcgcccac atatagggca tagcaaattt
    23281 attatccggg tcgtgtttgg caaccaactt cagcagttcc gggtcgagat tcttccactc
    23341 cggcaatttg cttttatcca gcggctggaa aactcccgca gtcaactggc gttcaagaaa
    23401 gcttgctgac ggcaccacca ggtcaaagcc ggtgctaccc gccattaatt tgccttccag
    23461 tacttcgtta gagtcgaaaa catcgtagac gactttaata ccggtttctt tttcaaaatt
    23521 ggccaccgtg tccggggcga tataatcaga ccagttataa atgtggagtg ttttttgttc
    23581 agccgcgagc gtgctgacag agacggccat cagagcaccc gcaaccagac ccgatagcca
    23641 ttttttattt aaggcggtca tatctctttc cttctgaaag ttcgttaaca aacgaacaaa
    23701 agttgtcaca ctgaaagata tgcaataaac gtgcatattt tttatcggcg cggtgtaaga
    23761 aaagagaact ctctcccggc cagcactgct cgctatataa aagcatcgca agaataactt
    23821 tagccagggt ttgagactat agctcggatt aaaaaacggt atttaacaaa tttttatgcg
    23881 tatttgctct atgtgataaa gccaaatcga cacaagagaa gtgaaacaat ctttaagaaa
    23941 agattaaaaa cagcagataa ttttgcagcg cgcagtcact gcaccagcga ctgcgtgctc
    24001 gaaaggagtt atcagtggag gaaatagttt tcagtaacgt tgttttgccc ttcatcgtca
    24061 gtcggtaaca gcagttgatg ggcattagct tcaagaatga ccatcgaaat ctgctcttcg
    24121 ctctgacgca cgaaccatgc aaactgctca taggttacgc cctgcataac ggataaagac
    24181 tggcaaacca ccagctttgg cagattatca tcctgcatat caagaaatgc tttcacggtc
    24241 agcgaactgg cattgatggc agataaatcc gccgccagcg gcaatacggc tgaaggtcgg
    24301 acttccgcca tggcagaaaa caggatcgtg ttatcgatca gatcgatttt ggcatcaaag
    24361 acaccgtcga aattctgcat atggggcaga tgcagagcct gacagttatc acattcaaaa
    24421 aaactcatcc ccaggtcatc gagccattga cgcagcgtat ccagaccagg aacgaccagc
    24481 gatgtcataa ttttgtacaa cctctttcga taaaaagacc ggcacagctt acgcaaaaag
    24541 cgcaggcaaa accatgatca gcaatgtgat tgcgactagc cacccgtttt caggcaatat
    24601 tctgtcgtag cgtggcgttc gatccagcgg atcattttac ccgcgatgtc gataccggtg
    24661 gttttttcta ttccttccag ccctggcgac gcattcactt ccatcaccaa cggcccgcga
    24721 ttagcacgca gaatatccac accagcaacg tccagcgcca tcgttcgcgc ggcttttatc
    24781 gcgatttcac gctcctgtgg tgtgattctg gcgacgcttg ccacgccgcc acgatgcaaa
    24841 ttggaacgaa aatcgccctc tttcgcccgc cgttcaatcg cagcgaccac ttcatcgcca
    24901 acaaccagac agcggatatc gcacccttgc gcctctttga tatattcctg caccagaata
    24961 tgcgcgttca gaccgcggaa agcgtcaatc acgctttccg ccgcctgacg cgtctccgcc
    25021 agcacgacgc caattccctg cgtgccttca accaacttga ccaccagcgg cgcaccaccg
    25081 accatgtcga ttaaatcgct ggtatcatcc ggcgaatgcg caatgcccgt gacaggcagg
    25141 tcgatgccct gacgcgccag cagttgcatc gaacgcaatt tgtcacgcgc ccgggcaatg
    25201 gcgaccgact cattgagcgg atagctcccc agcatctcga actggcgcag tgccgccgtc
    25261 ccataaaagg taatggcggt accaatacgc gggatcacag catcaaaatg gggtaacttg
    25321 cggcctttgt agtgaataga agacgccgca ggatttatgt tcatgtagca agaaagcgga
    25381 tcaagaattt caaccaggtg accgcgctgt atagcggctt cacgcagccg cttacacgaa
    25441 tagagcgttc catcccggga caatatggca attttcaccc tgcacctctc tgtcagacct
    25501 ggtcaaaagc ccgcgtatca tacatcggcg tggcggtttt agcgcgttgc ccaaccctgt
    25561 ttgtgcaaat aatccagaat aaatgggcgg ctttctttaa tgattgttcg gcggatatga
    25621 tcgctccagg tatcccggcg attattgctg ccacgggtga ggtaacattc cgccagttgt
    25681 tcgtcatact gcgccagtgc gtctttatcc agcggttgat agctgttttc atgcaccaaa
    25741 atggaggccg gtaaacgcgg cttaagatcc ggattatccg caggccagcc aaggcacagc
    25801 ccaaacaacg gcagaacatg ccgcggtaat ttcagcagct ccgtcaccgc ttcaatatta
    25861 ttgcgcaggc cgccgatata taccccgccc aatcccagcg attccgctgc ggttaatgca
    25921 ttctgcgcca tcattgccgt atcaacgaca ccgagcaaca gttgttccgc caggccaagt
    25981 tgtgcatccg gacagatctg taaatggcgg ttaaagtcgg cacagaacac ccagaactcc
    26041 gccgcttgcg ctacgtgttt ttgcccgccg gtcagcgtca ccagttcatc acgtaacgct
    26101 ttgtcggtaa tgcgaataat gctactgcac tgcaaaaaac tggaactgga cgtcgcacgg
    26161 gcgctgttaa taatcgcctc acgctgcgct tcggaaatgg gttcatcagt gaaatggcga
    26221 atggagcgat ggccacaaat aagttcaatg gttggcgtca ttatcttttt ctctttctga
    26281 acgtgaatat tgcggtggac ggttcatcag ctgtggggca agacgtttta ccacctgaag
    26341 aataaccacc accgcagcgg gaagcatgag caaaacaccg agaaaaatca tcagaatctg
    26401 cacttctggc cgagaaaatg gctcaggcag cgacaggggg tcgcttaccg acagcagcgc
    26461 caccgccagc gacatcattc cgataaattc cagtatcaac acgcttttag gcaatttacc
    26521 gatcgcgcgc atacgcttcc ctctgcaaag tgagccttca gtctaaaact tttcactgta
    26581 ttgtgtttaa cagttatagc ttttagcaat taatgcaaca ggttaaacct actttcagcg
    26641 aatacatttt agcgtgatca ttacaggcat aaatctatga ggagagaaat aatgcaaacc
    26701 gttatttttg gtcgtccggg ttgcccttac tgtgtgcgtg caaaagatct ggctgagaaa
    26761 ttgagcaatg aacgcgatga ttttcagtat cagtatgttg atattcgtgc ggaagggatc
    26821 actaaagaag atctacaaca aaaggcaggt aaacccgtag aaaccgtgcc gcagattttt
    26881 gtcgatcagc aacatatcgg cggctatacc gattttgctg catgggtgaa agagaatctg
    26941 gacgcctgat cgtctgacaa gccctcgcgt tgagggcttt attgattttt tctgtgctgt
    27001 ggtttaaaca aactactgat aaataagaaa cacagtgccc ccagcgcaca ccagaacacc
    27061 gcacttaata accatgccag ctcttgccag aatgagcgcg tcggtgaaaa aaacagccgc
    27121 ataataagca ttgaacaggg tgccgccagc attgcgccaa acagaggttt caggacttct
    27181 ctacgctgtg aaaagaagct ggcaactgct ccaggaagaa tgaaaaacag caagccgatt
    27241 tcaggatgcc cggcagcccg aaaagcgcct ttcatgtgcg tcgccagaaa aaggcacacc
    27301 acaatgaaga ggacaaaaca gcagattgcc cccgcccaac gttgtttatg tttcactcgt
    27361 tcctcctgac actgcgtcta tcgaacacat ttttcgccag tgtggcgttc agtaagataa
    27421 agccgcttcg cattccatgc taatataggc caacgcaatt catatagccg ttgataccta
    27481 atgtgattac actagtaaaa tatattgtta ctttactatc gtttaggtgc gctgaatgaa
    27541 tctgcgccct gaattctggt aaaaaacatt atcgtaaatt accatttctt tcaacagctt
    27601 actagtaaac aagaagttag cctccgtgaa tataaacgtc gccgaattgt taaatgggaa
    27661 ttacattctg ttattatttg tggtcctcgc gcttgggcta tgtctcggga aattacgact
    27721 tggttcgatc caactgggta attccattgg cgttttagtc gtatcgctgt tattaggcca
    27781 acaacatttc agcattaaca ccgacgcgct taatcttggc tttatgctgt ttattttctg
    27841 cgtcggggtc gaagccggac cgaacttttt ttccattttt tttcgcgatg ggaaaaatta
    27901 cctaatgtta gcactggtga tggttggcag tgcgctggtc atcgccttag ggttaggtaa
    27961 gctgtttggc tgggatattg gcctgacggc cggtatgtta gcaggctcta tgacgtcaac
    28021 accggttttg gtcggtgctg gcgatacact gcgtcattcc ggcatggaaa gcaggcagct
    28081 ctcactggca ctggataatc tgagcctcgg gtatgcctta acctatttaa tcggtctggt
    28141 gagtttgatt gttggtgcgc gttacttgcc gaaattgcag catcaggact tacagaccag
    28201 cgcccagcaa atcgcccgcg aacgtggcct ggacacagat gccaaccgta aggtttattt
    28261 gccggtgatc cgcgcctacc gcgtcggccc ggagctggtg gcctggaccg acggcaaaaa
    28321 tctgcgtgaa ctgggtattt atcgacaaac cggctgctac attgaacgta ttcgacgtaa
    28381 cgggattctg gcaaatccag acggtgatgc cgtgctacaa atgggcgatg aaatagcgtt
    28441 ggtaggctat cccgacgccc acgcccgact cgatcccagt ttccgtaatg gcaaagaagt
    28501 tttcgatcgt gaccttctcg acatgcgtat cgtcactgaa gaagtggtcg ttaaaaacca
    28561 taacgctgta ggcaaacgtc tcgcacaact gaagttgacc gatcacggtt gcttccttaa
    28621 ccgcgtcatt cgtagccaga ttgagatgcc gattgatgac aacgtcgtgc ttaacaaagg
    28681 tgacgtttta caagtcagcg gcgatgcccg tcgcgtaaaa accatcgccg atcgcatcgg
    28741 ctttatctcg attcacagcc aggtcactga cttgctggca ttctgcgcct tctttgtgat
    28801 tgggctgatg atcgggatga tcaccttcca gttcagcaca ttcagtttcg gcatggggaa
    28861 cgctgccggg ttgttattcg ccggaattat gctgggcttt atgcgtgcta accacccgac
    28921 cttcggttac attccgcaag gtgcattaag catggtgaaa gagttcggct tgatggtgtt
    28981 tatggcaggc gttggtctga gcgccggtag cggtattaat aacggcctgg gcgcgattgg
    29041 cggtcaaatg ttgattgccg gattgattgt cagtctggtg ccagtggtta tctgcttctt
    29101 gttcggtgct tatgtattgc gaatgaaccg cgcactgttg ttcggcgcaa tgatgggcgc
    29161 acgcacctgc gcgccggcaa tggagatcat cagtgataca gctcgcagta acatcccggc
    29221 gctgggctat gcgggcacct atgcaatcgc caacgtcctg ctgacgctag cagggacaat
    29281 catcgtcatg gtatggccag gattaggata aaactgaagt tgccctgaaa atgaaatttt
    29341 tttgcacaac cgcagaactt ttccgcaggg catcagtctt aattagtgcc actgcttttc
    29401 tttgatgtcc ccattttgtg gagcccatca accccgccat ttcggttcaa ggttggtggg
    29461 ttttttgttg cctgaaattt atctactacc ctgcaaccct ctcaaccatc ctcaaaatct
    29521 cctcgcgcga tagcggctta cggtcggtga caaaatgcag cgtcatcccc tcaataaacg
    29581 catcaagcgc gcgggcggtt ccgggttcaa accattgttc gagcgtttgc tgactgcgct
    29641 gcatccagtt ttgcattacc gtttttaata gcggttttcg gctagccagc gcgtagagct
    29701 ggtacatcag ctccatgtta tccggcgttg caacctgtga gctgtagatc atatcggtga
    29761 tagcctggca tgcgcccgga gcatcactaa catcgctaaa aaatgcctga tattgccggg
    29821 acatgatctc agtaaaacgg ctgaacgcct ccagcaacaa ctcatcaatt cctgaaaaat
    29881 agtaagtcat cgatcccaac ggtaccccgg caagggtagc aattttgcgg tgcgtaacag
    29941 catgtattcc gtaaagtttc accgcctcca gcgtggcctg gataattttt tctcgccgtt
    30001 gtggatcgtt agcgcgacgc atgtcatttc ctctgtctgc aattgtgtac aaatgtacac
    30061 aaccttgcta atgttgtctt ccctcttctt attttgacgt gatctatgac cgtaaattct
    30121 tcacgtaatg cattgaaacg ccgaacctgg gcgctgttta tgttcttctt tttgccaggc
    30181 ctgttaatgg cgtcctgggc aacccgtaca cctgctatcc gcgacattct ttctgtctcg
    30241 atcgctgaaa tgggtggagt cctctttggt ctgtcgatcg gttcaatgag cggtattctc
    30301 tgctcggcgt ggttagtgaa acgctttgga acgcgtaatg tcatcctggt cacgatgtcc
    30361 tgcgcattga tcgggatgat gatactaagt ctggcactct ggctgacatc gcccctgctc
    30421 ttcgcggttg gtctcggcgt ctttggggca agttttggtt ctgcggaagt ggcgataaac
    30481 gttgaaggtg ccgccgttga gcgagaaatg aataaaacgg ttttgccgat gatgcacggt
    30541 ttttatagcc tgggcacgct ggcaggcgct ggtgtcggga tggcactgac ggcctttggc
    30601 gttccggcaa cggtgcatat tttattggcg gcgctggtag gcatcgcacc tatttatatc
    30661 gccattcagg caatccctga cggtacgggc aaaaatgctg ccgatggcac ccagcatggc
    30721 gaaaaaggcg taccttttta tcgcgatatc cagttgctgt tgattggtgt tgtggtgctg
    30781 gcgatggcct ttgccgaagg ttctgccaac gactggttac ccttattaat ggttgacggt
    30841 cacggtttta gtcctacttc cggctcgctg atttatgccg gttttaccct ggggatgact
    30901 gttggacgct ttaccggcgg ttggttcatc gaccgttaca gtcgcgttgc cgtggttcgg
    30961 gccagtgcac taatgggggc gttgggtatt gggatgatta tttttgtcga tagtgcctgg
    31021 gtcgctgggg tgtctgttgt actttgggga ctgggtgcct cgttgggctt cccgctgacc
    31081 atttctgccg ccagcgatac cggccccgat gcaccgaccc gcgtcagcgt ggtagcaacg
    31141 accggttatc tggctttcct cgttgggccg ccgctgctgg gctatctcgg cgaacattat
    31201 ggattacgta gtgcaatgct ggttgtactg gcgctggtta ttctcgcggc tattgtcgcg
    31261 aaagccgtcg ccaaacccga taccaaaacg cagacggcga tggagaatag ttgatgagca
    31321 ttaaattaat tgcggtagac atggatggta ctttcttaag cgatcaaaaa acctataacc
    31381 gtgagcggtt tatggctcag tatcagcaaa tgaaagcaca aggcattcgc tttgtggtcg
    31441 ccagcgggaa tcaatattat cagttgatct ctttctttcc ggaaattgct aatgaaatag
    31501 cctttgtggc tgaaaacggc ggctgggtag tgagcgaagg caaagatgtt tttaatggcg
    31561 agctgtcgaa ggatgcgttt gctactgtcg tggaacattt gctgacgcgc ccggaagtgg
    31621 aaattattgc ctgcggaaaa aatagcgcct ataccctcaa aaaatatgac gatgccatga
    31681 aaacggtggc ggaaatgtat tatcaccgtc tggaatacgt cgataacttt gacaacttag
    31741 aagatatctt ctttaagttt ggtctgaatc tttccgatga actgattcca caagtacaaa
    31801 aagcattaca tgaggccatc ggcgatatta tggtgccggt ccacaccggc aacggcagca
    31861 tcgatctgat tatccccggc gtacataaag ccaatggcct tcgccaactg cagaaattat
    31921 ggggaataga cgacagcgaa gtggtggtct ttggcgatgg cggtaacgat attgagatgc
    31981 tgcgtcaggc aggctttagt tttgcaatgg aaaatgccgg cagcgcggtc gtcgcagcag
    32041 caaaataccg ggcaggctcc aataaccgtg aaggcgtact ggatgtgatc gataaagttc
    32101 ttaaacacga agcgccattt aaccaataaa aacggcagca cgcccggcaa cacaatgcct
    32161 gaatgaaggc tgcataattc gcggaaatta acccgctgca ttatttcagg cgaaatgatt
    32221 atgaaaaatt gtctactgtt gggcgcgctt ttaatgggct ttactggtgt ggcgatggcg
    32281 caaagtgtca ccgtcgatgt gccgagtgga tacaaagtgg ttgtggttcc tgattcagtc
    32341 agcgttccac aagcggtcag cgtcgcgact gtgccgcaaa ccgtctatgt ggctcctgcg
    32401 ccagctccgg cttaccgccc gcatccttat gttcgccatc ttgccagcgt aggtgaaggg
    32461 atggttatcg aacaccagat tgacgaccat caccattaat ttgagaatgc ctgatcgcac
    32521 aaagcaatca ggcatttttt tacccttcgt gcgaatttcc catctgttta tcttttaaaa
    32581 agataaccat cagcaacagc cacaaaatgc cgttgacaag gttgaagaga ttaaacagtc
    32641 cgttgccccc gttcagccag gcatgtttgc tgatttcaat accgacggta aagatcagca
    32701 tttgcagcat ccccatcgcc gcagaaaccg tacctttact catatcgctg gcaaacaggg
    32761 ttaatcgcac cagtcccgca ttcgccagac caataccgaa agcataaaga cttaacccgg
    32821 cggtcatcca taaatacgca tgcgatgaga taaccgttgc cgcagcagcg accaacagac
    32881 caatcataat cggccagccg cccataataa tcagcgaacg tacggtgcgg cgcgaggtca
    32941 gacgcgctaa cagcaagtta cccgcaatta acgccccgaa aataggcact tgcagcaaac
    33001 catattcata gctgctcaac tgctcgccgg taatgatgat aatcggcgac tgggcgatcc
    33061 acgccagcaa tggcaggcta acgaatccca gcgccagcgc ccccgccaca aagcgaccgt
    33121 tcttcagcac cagcttatag tcacgaccga gttctttcag cgacagtttc tcgcctatac
    33181 gcgtggcggt ttcaggcatg gctcgttgta gaccgaaaaa ggagatcgct gccaatgcgg
    33241 caaacaagac aaacatcccc tcccagggca gcacatggat ccacgccgcg cccaccagcg
    33301 gaccaagtag cggagcaatc agcgccacgt tcgccatcag cgcggtgatc ttgatacaaa
    33361 ccgcctcttc gaaggattcc tgaattgcgg cgtatcccac agcgccaatg aaacagaggc
    33421 ttatgccctg caagaagcgc aacagggtga attgttcaat gttttgcgcc agtaatattg
    33481 ccagacaggt gacgataaac cacaccactc ccgccagcat caccggacgg cggccaatac
    33541 gatccgacag cggccccagg agccattgta aaaacatccc gcccgccaga tacgcggtca
    33601 tcgaagtagg aacccaatca atgcccgcct gatattgttc caccaccgcc aacataccgg
    33661 gttgaatcat atcgttgccg atataggttg aaaattcgta aagcaccaga cagagaggga
    33721 aaagtaacgc ctgacgtcca agcctggcac cggaagctaa tttattttgc atgcaatttc
    33781 ttcgccaata ataatcgcgc aaagtttaat aaaagcgcag ctaacgagaa agcgaatttt
    33841 gtagctgaaa ccacggttaa gcacattctt acattattgc gagtatagct acgctttctt
    33901 taagttttat ttaacctatg cccgttacaa tcacccaccg taaacaggcc gcttgaggga
    33961 aataagacga tgccgcttta cccagtttaa cctgcacttt attctcaacg acttgcctgt
    34021 attggctccc ttttaatcac tttgcgtcgg gaagttatgc tggaaaattt gaatctctct
    34081 ctattctctc ttattaacgc gacgccagac tctgcgccgt ggatgatctc gttggcgatt
    34141 tttattgcaa aagatttgat taccgtgatg ccgctgctgg ccgcagtact ttggttgtgg
    34201 gggcttacag cacaacggca actggtgata aaaatcgcta tcgcgctggc ggtcagcctg
    34261 tttgtgtcct ggacgatggg gcatcttttt ccgcacgacc gaccctttgt cgaaaatatc
    34321 ggctataact tcctgcatca tgcggcggat gactcattcc cgagcgatca cggtacggtg
    34381 attttcacct ttgcactggc atttttatgc tggcatcgcc tgtggtccgg ctcactttta
    34441 atggtgctgg ccgtcgtcat tgcctggtcg cgcgtttatc ttggtgtcca ctggccgctg
    34501 gatatgctcg gtggattgct ggcaggtatg attggctgcc ttagcgccca gattatctgg
    34561 caagcgatgg ggcataaact ctatcaacgt ctgcaatcgt ggtatcgctt ctgttttgca
    34621 ttaccgatcc gcaaaggctg ggtgcgtgac tgatctctgc taaaaagtgt agtattgagc
    34681 ggctcgcttc aataactatt cagagggatt atggaaacac gtcgcgaaga gcgtatcggg
    34741 cagctgctgc aagaattaaa acgcagcgat aagttacatc ttaaagacgc cgccgccctg
    34801 cttggggttt cggagatgac gattcgtcgc gatctgaaca accacagtgc gcccgtcgtt
    34861 ttgctcggcg gctatattgt tctggaaccg cgcagtgcca gccattacct gttaagcgat
    34921 caaaaatccc gcctggtgga agaaaaacgc cgggcggcaa aactggctgc gacgatggta
    34981 gaacccgatc agaccctctt ttttgactgt ggcaccacca caccgtggat tattgaagcg
    35041 attgataatg aaatcccttt taccgccgtt tgttattcgc taaatacctt tctggcgctg
    35101 aaagagaaac ctcattgccg cgcgtttctg tgtggtggtg aatttcacgc cagcaacgcc
    35161 attttcaaac ccatcgattt tcagcaaacg ctgaataatt tttgcccgga tatcgctttt
    35221 tattctgcgg cgggcgtgca tgtcagtaaa ggcgctacct gttttaatct tgaagagttg
    35281 ccggtaaaac actgggccat gtcgatggcg caaaagcatg tgctggttgt cgaccacagt
    35341 aaatttggca aggtgcgtcc ggcgcgcatg ggtgacctga aacgctttga tattgttgtg
    35401 agcgattgtt gcccggaaga tgagtatgtg aagtacgcgc agacgcagcg tattaagttg
    35461 atatattaat gacgtataac cggatgacgt ttcgcgccat ccggttatca gaagattaag
    35521 agaaccagct gccgaaccac tgatggaatt tcatcatcac gaaatcccac atccgaccaa
    35581 agaatccgcc ctcttccaca ttttccatca cgatcagcgg acgctgctca atggatttac
    35641 cgttaagctg gaaatcaatg gtcccgacaa cctgaccttt tttcagcggt gcggtaagct
    35701 gcggttccgt taacgtataa ctcgctttca ggtttttcag ctgcccacgc ggtatggtca
    35761 ctgagcccgc ttcacctgcc ccgagattca cttcgctctt atcaccaaac cagacgcgct
    35821 gagtcacaaa ggtggcatca ggtttaattg gcgtcacggt ttcaaagaag cggaaacccc
    35881 aggtcaataa tttctcagac tcattaaaac ggatacggtc agttttcgcc cccagcacta
    35941 cggagattaa acgcatatca ccctgggtag ccgaagcaac cagattatat cccgcgcctg
    36001 cagtggttcc tgtcttcatg ccatcaacat tcagattgct gctccacagc agacggttac
    36061 ggttaggctg acgaattttg ttgaaggtga actctttctc tttatgaatg gcgtactctt
    36121 ccggcacatc gtggattaat gctttaccca gcaatgccat atcgcgcgcg gtactgaact
    36181 gccccggcgc atccagaccg tgcaccgtct ggaaggtagt gttggtcaga cccagttttt
    36241 tggcataacc attcatcaaa ccaataaatg actcctggct cccggcaacg taatcagcca
    36301 gcgcaataca ggcgtcatta ccggactgga taatcacacc tttgttcaag tctgccaccg
    36361 aaacctgatc gcccggtttg aggaacatta ccgatgaacc acgcagtgcc ggatttcccg
    36421 tcgcccaggc atctttaccg accgtcacca tatcggtgag tttaatctta tcggccttaa
    36481 gcgcctgccc aaccacatag ctggtcatga ttttagtcag gctcgcggga tccagtttct
    36541 catccgcgtt gccttctgcc agcactttac cgctggcgta atccattaaa atccatgcac
    36601 gcgcatccac gctcggcgct tcaacggttt gttccgccgc gaatgccgtt ggggcaaaaa
    36661 ggaataaaaa tgcagaaccc gctgcaagac cacgaaggag agaggagtat tgcgtcataa
    36721 atgccacccg agtatccatt caaaaaaaat acgttatatc gccgtatgat aagttcggtg
    36781 attaataacg cacacacaaa cttaaagaaa ccgtaagttg gtaaagtttt taaagtttat
    36841 gcaatatcct gccgcttcgc tgcttctggt gtcatttttt tgccagctgt tgcttaaaag
    36901 ttaactttac ttcaccatcg gagtcctgtt cagcctgtgg ctgcctgtcg gaaaaggggt
    36961 gagtatgatt acgctgtggg gtcggaataa ttcaaccaac gtaaaaaaag tattgctgac
    37021 gctcgaagaa ctggaactac cttatgagca aattctcgcg ggccgtgagt ttgggataaa
    37081 tcacgatgct gattttctgg cgatgaaccc taacgggctg gtgccgttgt tgcgtgacga
    37141 cgaaagtgat ctcattcttt gggaatcaaa cgccattgtc cgctatctgg cggcacagta
    37201 cgggcaaaaa cgcctgtgga tcgactcacc ggcacgtcgt gcggaagcag aaaaatggat
    37261 ggactgggca aaccagacgc tcagtaatgc ccatcgcggg atcctgatgg gattagtcag
    37321 aacaccaccg gaagagcgcg atcaggccgc cattgatgcc agttgtaaag agtgcgacgc
    37381 cctgtttgcc ctgctcgatg cggaactggc aaaagtaaaa tggttctccg gcgacgagtt
    37441 tggtgtgggc gatatcgcta tcgcaccgtt catctacaat ttgtttaacg ttggcctgac
    37501 ctggacaccg cgtccaaatc tgcaacgctg gtatcagcaa ctcactgaac gccccgcagt
    37561 gcgcaaagtg gtgatgattc ccgttagcta attgcgtggg ctaactttaa gtaattcccc
    37621 actggactcg tcggtgagaa cgtataaata accgtcgggt ccagtgcgaa catcacgaat
    37681 tcgctgccct ctgtccgtta aaatacggcc atcttctgtc actttgtcgc cgttgacgct
    37741 catcacaatg acatctttat ctttcagcgc gccaataaat aatttttgct gccactgggg
    37801 gaatttatcg ctgttataga aggccatgcc gctcacggcg ggcgaatctt tccagtaaaa
    37861 aacaggttgc tcggtcccgg cgacgatctc ccctttcgct tccggtattt taaagcccga
    37921 atagttgatt ccccaggttg ccagcggcca gccgtagttt ttgccttttt gcgggatatt
    37981 aatttcatcg ccaccgcgcg ggccatgttc attcagccat agtgcattac tccacggatt
    38041 catcgccatt ccttgcggat tacgaatgcc ataagaccag atctcggcgc gtgcaccgga
    38101 ttcctttata aaaggattat catccgggat ttcgccctgg tcggtcagac gcaccagttt
    38161 gccctgtaat ttatccagat cctgcgccgt cgggcgctga ttgttttcgc ccagagcaat
    38221 aaaaagataa cctttaccgt cgaataccag ccgcccgcca aaatggttgc cggtagacag
    38281 ttttggcatc tggcgaaaga cggtgcggaa gtcggtcacc tttgagagat catcacttaa
    38341 gcggccataa cccacagcag ttccggcttt gccatcatcg ccaacttcgg aataacttaa
    38401 ccagatgcgg cgagactgag caaaatcagg cgctaaaacc acatccagca ggccaccctg
    38461 cccgtgcgcc caaacttccg gcactccgga aagcggcgca gataatcctt ttcctgcttg
    38521 ccagtgacgc aactcgccgc cgcgcagagt gattaacata ccgtgattgt cgggtaaaaa
    38581 ggccagcgcc cagggatggt cgagtttgtc ttgcagtact tcgacattta ccgttgcagg
    38641 agccgcccag agagcggaag aaagacaaat aaggggcaca aggaaaaagg attgtcgatg
    38701 cataacgcct ccggattgac tctggcttaa agcgtagtca gtggaggaga taacaaattc
    38761 atttttacaa aaacttaaac atgaaggggg agacgctttc tcccccttag ttttcaggcc
    38821 ttctcaagca tagcgtgctt ctgcaggctc tggatactca gcgttaagct catcagacaa
    38881 ttttcaagct tatcggcgtt gacggtaata acagtcgggc aatcatggtg cccactcatc
    38941 aaacatactg cggctgtcgc taatgcttct tcagcatgat gaagagcact ccactcttcc
    39001 tgatccagat gaagattcaa ccgcagcgat ttatcgtgca gttcgcgatt cagtttaaaa
    39061 aagttatctc gtagatgatt gctttcgctg acggacatgt atccttttgc cttcctcagt
    39121 tgcacgtatg cggcgaggtc aacgcgtgcg tcgatcaacc ggttcagcag gtcgattcgc
    39181 tgtctgtcaa cgaacatact tcgatcctcc tctttccagc cttgttgcag ttaaatagta
    39241 tggctatttt ttgagcaaaa tgctgtccag gatcaattct tcacgccgaa ttaacaaaat
    39301 ttacatttcc ttcgcctccg tcagcgaatg agattgcgat acaaatttta tccagaactc
    39361 tgtagaatcc ctgccctgac ctttcctgta actgaatatt ttttaagcta tgagcaaagt
    39421 aactccccag ccgaaaatcg gctttgtttc ccttggctgt ccgaaaaacc ttgtcgattc
    39481 agagcgtatt ctcaccgaac tacgcactga aggttatgac gtggtaccga gctatgacga
    39541 tgcggacatg gtgatcgtca acacctgcgg ctttattgac agcgcggtac aagaatcact
    39601 ggaagccatt ggtgaagcgt tgaatgaaaa cggcaaggta attgtgaccg gttgtctggg
    39661 ggcaaaagaa gatcagatcc gcgaagtcca cccgaaagtg ctggaaatca ccgggcctca
    39721 tagctatgag caggttctgg agcacgttca tcactacgtg ccaaaaccga aacacaaccc
    39781 attcctgagc ctggtgccag aacaaggtgt gaaactgaca ccgcgtcatt atgcctatct
    39841 gaaaatttct gaaggctgta atcaccgctg caccttctgc attattccgt ctatgcgcgg
    39901 cgacctggtg agccgtccga ttggcgaagt gttaagtgaa gcgaaacgtc tggttgatgc
    39961 gggcgttaaa gagattctgg tgatctcgca ggatacttcc gcgtatggcg tcgatgttaa
    40021 acatcgtact ggcttccaca acggcgagcc cgtaaaaacc agcatggtca gcctgtgcga
    40081 acagttatcg aaactgggga tctggacacg tctgcactac gtttaccctt atccgcatgt
    40141 ggacgacgtc atcccactga tggcagaagg caaaatcctg ccgtatctgg acattccgtt
    40201 gcagcacgcc agcccgcgca ttctcaaact gatgaagcgt ccgggttctg tagatcgcca
    40261 actggcgcgc atcaaacagt ggcgcgaaat ctgcccggaa ctgaccctac gctcaacctt
    40321 tattgtcggc ttccctggcg agacggaaga agatttccag atgctactcg acttcctgaa
    40381 agaagcacgt ctggatcgcg ttggctgctt taaatacagc ccggttgaag gtgcagacgc
    40441 caatgccctg cctgaccagg ttccggaaga agtgaaagaa gaacgctgga accgtttcat
    40501 gcagttgcag cagcagattt ccgccgagcg cctgcaagag aaagtgggcc gtgaaattct
    40561 ggtgattatc gacgaagtgg acgaagaagg tgcgattggt cgcagcatgg cagatgcacc
    40621 ggaaatcgac ggcgcggttt atctcaatgg tgaaaccaac gttaagccgg gtgatatcct
    40681 gcgtgtgaaa gtcgagcacg ccgatgagta cgatttgtgg ggtagccggg tttaattaat
    40741 cttaaggcag atctaacaag gatattgttc ttcgatctgc ctctgctacc cttcccgatg
    40801 tcctaccctt acgcacaaaa tcccctgacg cataaatcga gaaaaatgta tcgtactcga
    40861 ggcaactctc actttttcac accaaattaa agttttgatc gcaattactt gaaataagcg
    40921 taatggttac tatagtaagc attaccacaa ggatggatgt atgggtgtgt ataaggcaag
    40981 acgattcagc caaagcacga aaaaacttgg tattcacgat aaagttctca tggctgccgc
    41041 tgaagaagtg atgcagggca tatgggaggc tgatttaggt agcggtgtca tcaaaaaacg
    41101 tttacccctc caacaaggaa agagtggtgg agccagaaca attatctttt ttaaatcagc
    41161 aaatcatgtg tttttttatg atggttggtc caagagtggt ctaagcagta aaggaaccaa
    41221 agaaatcgaa gatgatgaac tggcggccta caaaaaaatg gcaaatgcat ttctggcctt
    41281 tagcaataag caaatagagg atttgatcga aactggcttt ttgattgagg tgaaaaatga
    41341 gcgataaatt acaaatgatg ctggaagatg caaaggaact gaatgcactg ggcctgatgt
    41401 ctgatagtga agttgatgcc attgccgtca tggtcaaagc tcgtgaagtc agcgcccgta
    41461 tagccaaagt caaatcaatg acgggtgaag agatcaaagc catccgcacc cgatacggca
    41521 tgtcacaatc cgtgctggca catacaatgg gaatgtcaaa agaaagtgtc tcgaaatggg
    41581 aacgtaatga aataaaaccg agcggcccgg cattacgtat tttgaataca ttggcaataa
    41641 aagggccgga agtttttgca aagtaaaatg tttgcaaatg atttaccaca ttcattagta
    41701 ccccttgatt aaggggtact aacactattc taattctact gccagctaat ctgcgccctg
    41761 ccgtttttct tactcttata cagccgctca tccgccagca ttttcaagcg ttcgacggta
    41821 ggacattcat ttgaatgcgc tacaccgata ctggtactta aattaatgct tcccacctgg
    41881 aaatgaacga tttcttttag aatcgatagc aactcttcca gcgttccacc cgtataaatc
    41941 accgcaaatt cgtcaccacc gaagcggtag actttacctt tatctaccgg gatctgtgat
    42001 ttaaggattt tcaccacttt catcaacact tcatcgccca gcaaatgacc gaaggtgtcg
    42061 ttgatgtttt tgaatttatc gacatcaaaa agacacagat agccgccttt ttccggcagg
    42121 gcttttaaat cttcgtcaaa ggattttctg cccattgctt tggtcaggtc atccaggaac
    42181 gccgagttat acagactgta gagttttttg acatcggcag ccgaaaagaa aagcccgcca
    42241 tccagtacgc gttcaaaatg gttattaatg atggcttctt tctccacaac accacgatta
    42301 atcactctgc gtaccagata gaggcaactg gcacccgata tcactgccac caatgcggca
    42361 aagaaaataa tcaatgtaca gacccgcaaa taaacatctt ttttatacgc aaatacgtac
    42421 aatccggtcg gcaaataggc gaagtgctcc atcgcttccg aaacggtgaa gatggcgttt
    42481 ttagcattat attcatcacc ggtcaccggt ttaccgttct taaacagaga gaacatcagg
    42541 ttctcgctgt ccatcgccag cgattttgac agtaaagaaa aacgttccag accaagcaaa
    42601 aatatcagac tgcgattgtg agttaccgga tagaggtaat agacgaaggt tttaccgtca
    42661 aaagagataa accctgtatt tttgacatgt cgatacttca caacctgcat aatatctgca
    42721 caataatttt tctcgaactc ctttacctga atacattcgt cactggaaat cagataagga
    42781 ccgacatcgt acaaataaga gggtgaaagc gagtcggcgt aagctgtgcg taagccaacc
    42841 aacagctgtt cctccgtagg atttagcgtg ttgatcagat caattgccac actacgccga
    42901 tattcagcaa tatttgccac cgcaaaagca gtatcaataa cggactgttt caggccatac
    42961 acccgttcct ttaccatttg agtgtagatc ccgcaggcaa ctgctgaaat catgataaaa
    43021 atcagcagac tgactgtaag tacgaattta ttgattctgg acatacgtaa actactcttt
    43081 tactaatttt ccacttttat cccaggcgga gaacggcatg ggtttctggt acagataccc
    43141 ctgagcctga acacatccta atttgattaa ttcgcgcgca acgtcttcgc tctctacgcc
    43201 ttccgcaatc accgggatcc ggttatagcg tgccagatcg aaaataatgc gcacaatttt
    43261 tcccgcagta ccgaaaatat cgccggtaaa ttgtgagtcc acttttatgc tatctggatt
    43321 aatttcataa aaacgtttca ggtttgacaa accggtgcca aagtcatcaa tcgatatccg
    43381 taccccttgt ttacgcagtt gttcaatatt gagataaaaa tgttcattaa ttcgctcaat
    43441 cacggtttca gtgatctcaa cacagaggct gttcaccttc aggccatttt cttccatgat
    43501 ggtggtaaaa cgctcatgaa agtgtggttc gtttaactgc cagggcgaaa cattaatatg
    43561 cagtaagaaa ccgtccttca ggaccgcacg ctgactaaaa tgtctgaatt cacccatcgc
    43621 ggttttaata atctgataac ccagctcatt gataaacccg atatcttcag caatggtaat
    43681 gaactttagc ggtgaaatga tcccccgctc cgcagacacc caacggcaca acgcctccgc
    43741 gcccagaatg gcgcgggtgt taatatcaac gatcggttga tagacgagga atagttcgcc
    43801 aatttccatt gcatggcgaa tattacgcgc aatccagata ttctcttcta tttcgttgag
    43861 tttcgcatca tcacagaaaa taaactctga gaacatatgg ttgcggatac tggaaatcgc
    43921 cagtttcagc ttttctgcgt aactccaggc gttttccccc tgcaaaggtt cacaaatagc
    43981 agacttatgg atgcgcagat tgcggtagcc aaaactgctg tcaaaatcgt ttaccgtc
//
LOCUS       DAMZFT010000021        43206 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.021, whole genome shotgun sequence.
ACCESSION   DAMZFT010000021 DAMZFT010000000
VERSION     DAMZFT010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 43206)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43206)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43206
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.021"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..608
                     /locus_tag="PN977_001341"
     CDS             <1..608
                     /locus_tag="PN977_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HDI6041393.1"
                     /translation="AMRVSPLGCLLPARDIDSFIDDVALASSPTHKSDLAVAGAVVIA
                     WAISRAIDGESWSAIVDSLPSIARHAQQKRITTFSASLAARLEIALKIVRNADGTESA
                     SEQLYQVVGAGTSTIESVPCAIALVELAQTDPNRCAVLCANLGGDTDTIGAMATAICG
                     ALHGVNAIDPALKAELDAVNQLDFNRYATALAKYRQQREAI"
     gene            605..1570
                     /locus_tag="PN977_001342"
     CDS             605..1570
                     /locus_tag="PN977_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HDI6041394.1"
                     /translation="MSGARLHTLLPELTTRQPVMVVGAAVIDVIADAYALPWRGCDIE
                     LKQQSVNVGGCALNIAVALKRLGIEAGNALPLGQGVWAEIIRNRMAKEGLISLIDNAE
                     GDNGWCLALVEPDGERTFMSFSGVENQWNRQWLARLTVAPGSLLYFSGYQLASPCGEL
                     LVEWLEELQDVTPFIDFGPRIGDIPDALLARIMACRPLVSLNRQEAEIAAERFALSAE
                     ITTLGKQWQEKFAAPLIVRLDKEGAWYFSNDASGCIPAFPTQVVDTIGAGDSHAGGVL
                     AGLASGLPLADAVLLGNAVASLVVGHRGGDCAPTREELLLAHENV"
     gene            complement(1544..2290)
                     /locus_tag="PN977_001343"
     CDS             complement(1544..2290)
                     /locus_tag="PN977_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDI6041395.1"
                     /translation="MEQAHTQLIAQLNERILAADNTPLYIKFAETVKNAVRSGVLEHG
                     NILPGERDLSQLTGVSRITVRKAMQALEEEGVVTRSRGYGTQINNIFEYSLKEARGFS
                     QQVVLRGKKPDTLWVNKRFVKCPEEVAQQLAVEAGSDVFLLKRIRYVDEEAVSIEESW
                     VPAHLIHDVDAIGISLYDYFRSQHIYPQRTRSRVSARMPDAEFQSHIQLDSKIPVLVI
                     KQVALDQQQRPIEYSISHCRSDLYVFVCEE"
     gene            complement(2342..3160)
                     /locus_tag="PN977_001344"
     CDS             complement(2342..3160)
                     /locus_tag="PN977_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
                     /protein_id="HDI6041396.1"
                     /translation="MQLRITSRKKFTVLLCALGLISIVAIYPRQTVNFFYSTAIQIKD
                     YIHFYGYRPVKSFAIRIPASYTIHGIDVSRWQERIDWQRVAKMRDNGIRLQFAFIKAT
                     EGEKLVDPYFSRNWQLSRENGLLRGAYHYFSPSVSASVQARLFLQTVDFSQGDLPAVL
                     DVEERGKLSAKELRKRVSQWLKMVEKRTGKKPIIYSGAVFYHTNLAGYFNEYPWWVAH
                     YYQRRPDNDGMAWRFWQHSDRGQVDGINGPVDFNVFNGTEEELQGFVDGIKETP"
     gene            complement(3225..4025)
                     /gene="thiD"
                     /locus_tag="PN977_001345"
     CDS             complement(3225..4025)
                     /gene="thiD"
                     /locus_tag="PN977_001345"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HDI6041397.1"
                     /translation="MKRINALTIAGTDPSGGAGIQADLKTFSALGAYGCSVITALVAQ
                     NTRGVQSVYRIEPDFVAAQLDSVFSDVRIDTTKIGMLAETDIVEAVAERLQRYQIQNV
                     VLDTVMLAKSGDPLLSPSAVATLRSRLLPQVSLITPNLPEAAALLDAPHARTEQEMLE
                     QGRSLLAMGCGAVLMKGGHLDDEQSPDWLFTREGEQRFTAPRIMTKNTHGTGCTLSAA
                     LAALRPRHTNWADTVQEAKSWLSSALAQADTLEVGHGIGPVHHFHAWW"
     gene            complement(4022..4810)
                     /gene="thiM"
                     /locus_tag="PN977_001346"
     CDS             complement(4022..4810)
                     /gene="thiM"
                     /locus_tag="PN977_001346"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HDI6041398.1"
                     /translation="MQVDLLSSAQSAHALHLFHKHSPLVHCMTNDVVQTFTANTLLAL
                     GASPAMVIETEEASQFAAIASALLINVGTLTQPRAQAMRAAVEQAKRSQTPWTLDPVA
                     VGALDYRRRFCLELLSHKPTAIRGNASEIMALAGVANGGRGVDTTDAAANAIPAAQTL
                     ARETGAIVVVTGEVDYVTDGHRIVGIHGGDPLMTKVVGTGCALSAVVAACCALPGDTL
                     ENIAFACHWMKQAGERAVARSEGPGSFVPHFLDALWQLTQEVQA"
     gene            complement(5600..5881)
                     /locus_tag="PN977_001347"
     CDS             complement(5600..5881)
                     /locus_tag="PN977_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001353111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6041399.1"
                     /translation="MTETDLLKIIRRITGISQQPDEQATQPDSVIAENYARVVAEVMR
                     RDGIELNGVDMRNIRTRVLELLAYRRRSQQRRESAKNTYQWRKPEHLRR"
     gene            complement(5887..7548)
                     /locus_tag="PN977_001348"
     CDS             complement(5887..7548)
                     /locus_tag="PN977_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HDI6041400.1"
                     /translation="MTAWHEYAEGVKNGKITACKRLKQAVKRYFSDLENSLYTFDPEV
                     VERFIAFSRVCPHVKGAMRGSPIELEPWQQFAFACILGFKVKATGRRKYTSAFIEVPR
                     KNAKSTVAAILANWFLVMENGQQDIYTAAVSRDQARIVFDDARQMCLLSRPLRKRVNI
                     QAHKVIHPKTNSLLKPLAAKAATIEGTNPSLAIVDEYHLHPDNGVYSALELGMGARPE
                     GLLFAITTSGSNVVSACKQHYDYCCQILDGEEVNESMFVLIYELDDESEVDDPAMWIK
                     ANPNIDVSVDREKLASTIQKARGIPSQWVEMLTKRFNIWCQGATPWMGNGAWAECAGT
                     FAEADLYGQECYAGLDLSSTSDISSVCYAFPVGKKIMLVSRHYLPEFQLQNPANKNRA
                     IYRQWAKAGWIRTTPGDCIDYDRIRDDIMADAENFNIRLVGFDTWNATHLRTQLQGAG
                     FEVEPFPQTYLRFSPAAKSFEVFVNRKVIVHRGDPVLAWSMSNVVMQSDANANIKPNK
                     KKSSNKIDPSVAALMAFGTFQAEHEEFAFDMSDSQKERLAAFDGV"
     gene            complement(7532..7888)
                     /locus_tag="PN977_001349"
     CDS             complement(7532..7888)
                     /locus_tag="PN977_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001353110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HDI6041401.1"
                     /translation="MARPPKAPAYLDEIAVRQWKEKSRQLSGREDLTPADWSNLELYC
                     VNYSIYRKAVEDLATRGFSIVNSQGSESRNPALSAKADAERIMIKMASLLGFDPVSRR
                     RNPPETEEEDELDRLA"
     gene            complement(8008..8193)
                     /locus_tag="PN977_001350"
     CDS             complement(8008..8193)
                     /locus_tag="PN977_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001251183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6041402.1"
                     /translation="MAMMTEQEQTRLIRGLIRQRDTWKTQETEHKANRTGRTKRTTAK
                     RLTDRDREVMECFRNRW"
     gene            complement(8177..8617)
                     /locus_tag="PN977_001351"
     CDS             complement(8177..8617)
                     /locus_tag="PN977_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707053.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HDI6041403.1"
                     /translation="MPWQPLRRCTEPGCNKRVKSGKCEEHRRAAWRAEDARRGHRRAR
                     GYSRQWDKYRALYLSKNPLCVRCLAKGIYTPALVVDHIIPINGGGDVLFWPEWNHQAL
                     CQTCHNRKTTREDPATKANRKAGMYREQEERAAHRNDWMYGDDD"
     gene            complement(8617..8919)
                     /locus_tag="PN977_001352"
     CDS             complement(8617..8919)
                     /locus_tag="PN977_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001287546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HDI6041404.1"
                     /translation="MSEARITPDEVRAHLRLDDDLSGEGELLKMYTDAALEACQKHIG
                     KRFEDGLEFTPAMRVGCLMYIAFLYENREAVSPVEHSELPMAISALWSVYRDVGVY"
     gene            complement(8912..10126)
                     /locus_tag="PN977_001353"
     CDS             complement(8912..10126)
                     /locus_tag="PN977_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001713427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HDI6041405.1"
                     /translation="MWWPFSRKKSEQRNLSIDDFLALSGVPNTGSGEYVSAGTAESLP
                     AVMNAVSVIAEAVATMPCYLYLVRNDKGREAREWLDSHPVDILLNEQPNSCQTPYQFK
                     RTMMRHCLLNGNAYAVIEWGQDGQPKSLHPYAPGCVVPERTGAHKYRYTITEPCTGTV
                     RTYLQEEVLHLRYASDDGFLGRSPVTICREALGLGLAQQRHGASIMKDGMMAAGIITS
                     GEWLDGVKGKQALDALERYKGAKNAGKTPILEGGMDYRQLGMSNQDAEWLASRRFSIE
                     DIARMFNVSPIFLQEYSNSTYSNFSEASRAFLTMTMRPWLANFEQQIKAALLVASPVP
                     GTRYLVEFDSADLLRATPTERYATYEKGIKNGIMNPNEAREREGMPPREGGDEFSQAW
                     KQTVEIKGRKDE"
     gene            complement(10128..10688)
                     /locus_tag="PN977_001354"
     CDS             complement(10128..10688)
                     /locus_tag="PN977_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HDI6041406.1"
                     /translation="MKNTDFEIRTSELTASNKKLVGYAVRWNSLSEIIWDEFREQFTP
                     GAFADYLATGNDVRCLYEHDYTQLLGRTKSGTLVLTEDNTGLRFELTPPDTQLGKDVL
                     TLVERGDITGMSFGFRALCEEWSIAQKPYLRTVTAAELREITITSMPAYPESGVEIAH
                     RSLFAQHPELRPTGNNRHRWSELAGL"
     gene            complement(10743..11912)
                     /locus_tag="PN977_001355"
     CDS             complement(10743..11912)
                     /locus_tag="PN977_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000733253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HDI6041407.1"
                     /translation="MKKLIELRQQKNALKNQMRSLLEKADSENRSLNAEEGKQFDELR
                     AKADALDTEISRLESVADEERSKPGTGIQKLSSDELRNYIVTGDVRSLSTSTDSGRDG
                     GYTVIPELDREVMRQLQDDSVMRVIATVKTAKSNEFQKLVSTGGATVGRGTEGSARSE
                     TNTPKIERVTIKLNPIYAYPKTTQEILDFSEVDILGWLSSEIADTFASTEEDDFVNGD
                     GNGKPKGFMAYTRAATSDKTRAFGTIEKIVAASGTAITADELIDILYKLKAKYRKNAV
                     WVMNSGTAGTLQKLKNENGDYIWRDSLKEGAPDMLLGRPVYCLESMPDIGAGKAPLAV
                     GDFSRGYFIVDHVTGIRTRPDNITEPGFYKVHTDKYLGGGVVDSNAIKILEMKAG"
     gene            12199..12705
                     /locus_tag="PN977_001356"
     CDS             12199..12705
                     /locus_tag="PN977_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001053663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6041408.1"
                     /translation="MNLSAMVYPDTFIINGESFRGKRNAKENKVLIPYTNEPEVTIGQ
                     HIIQRVGKNEINLKIIDMKLLPNGTRRQGTNHPNMLTLYIENITGNEHMTPTKSNTFN
                     IGSISGDQVQIGEHNHMLVNISITELVEKVAKSGDVQAKSVLKQLLENSTVASIVGAG
                     ASALIGLL"
     gene            complement(12741..12989)
                     /locus_tag="PN977_001357"
     CDS             complement(12741..12989)
                     /locus_tag="PN977_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001145408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6041409.1"
                     /translation="MPVTFEEVQQHKKFHDFDDLETMTAKKYRRLLSSDALFVVDHHD
                     FLRSSLTGEIFATNREQVEAMIEYLWKIRRRMRDPVKR"
     gene            complement(13006..13431)
                     /locus_tag="PN977_001358"
     CDS             complement(13006..13431)
                     /locus_tag="PN977_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001758993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HDI6041410.1"
                     /translation="MTAQIAAYGRLVADPQLKTTSKGTQMTMASMAVPLPCSQADDGT
                     AMMWLSVLAFGRQADALAKHHKGELVSVAGNMQVSQWTGQNGETRQGWQVIADSVISA
                     RTARPGGKKGQQGQATDALNRAKQQTGQHDDPYGDNIPF"
     gene            complement(13428..13673)
                     /locus_tag="PN977_001359"
     CDS             complement(13428..13673)
                     /locus_tag="PN977_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6041411.1"
                     /translation="MTAKHTKKSQSHALDLTEHWLRVSIKIIDRNAGEGYAKAHPELI
                     SAFMTTAAANFATLTEREIAEAEQVTTINVKTGEQTA"
     gene            complement(13961..15778)
                     /locus_tag="PN977_001360"
     CDS             complement(13961..15778)
                     /locus_tag="PN977_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000710169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HDI6041412.1"
                     /translation="MKKAPNLKHQPRDKMTEVIIFAGSDAWAHAKQWQEQDGRLAGDN
                     VPPVWLGEQQLAELDNLQIVPDGRYRVRLYQAGLLRPGLVNTIGQKLAVAGVRDADYY
                     PEGMHSQKRENWREYLERERAEQAEKKKVVELPVKKKERVKDDNASSLALNQMGASQR
                     GEVLLAHYGGELAIHADSDTVHHYNGVVWEPVQDKELQRAMAQIFIDAEISYSQNAIK
                     SAVDTMKLSLPVMGNTARNLIGFSNGVFDTRTGNFREHNKNDWLLIASELPFSPPAEG
                     ETLATHAPNFWKWLRRSVAENDRKADRVLAALFMVLANRYDWQLFIEVTGPGGSGKSV
                     MAEICTMLAGKANTVSASMKALEDARERALVVGFSLIIMPDMTRYAGDGAGIKAITGG
                     DKVAIDPKHKAPYSTRIPAVVLAVNNNAMSFSDRSGGISRRRVIFNFSEVVPENERDP
                     MLAEKIEGELAVVIRHLLTRFADQDEARRLLYEQQKSEEALAIKREGDSLVDFCGYLM
                     ASVMCDGLLVGNAEIVPFSPRRYLYHAYLAYMRAHGFGKPVTLTRFGKDMPGAMAEYG
                     REYMKRKTKHGFRSNVTLTEESEDWMPSCVSVTNDDSKN"
     gene            complement(15775..16074)
                     /locus_tag="PN977_001361"
     CDS             complement(15775..16074)
                     /locus_tag="PN977_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HDI6041413.1"
                     /translation="MRTYLSGLTASGYAHPQIIPGAIYLDKNGNRVTVKELMFDRVYF
                     IRDGYSFLSSLNVEIFISRFSREIPLSRNNHVSCMDVDKKLQELKNMIAAWREQK"
     gene            complement(16081..16401)
                     /locus_tag="PN977_001362"
     CDS             complement(16081..16401)
                     /locus_tag="PN977_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001113145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6041414.1"
                     /translation="MPDMSNYQYLINPHFNCEHDIAKKVYSAADGATDNISMAVASIG
                     SLMWHASENEDYDEKAMRIDMGNIGLLLAMLGHFDISLRCTIENATDALNAIKKANTD
                     SNRG"
     gene            complement(16394..17393)
                     /locus_tag="PN977_001363"
                     /pseudo
     CDS             complement(16394..17393)
                     /locus_tag="PN977_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001323528.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
     gene            complement(17446..17670)
                     /locus_tag="PN977_001364"
     CDS             complement(17446..17670)
                     /locus_tag="PN977_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001075374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family phage regulatory protein"
                     /protein_id="HDI6041415.1"
                     /translation="MNQNTQFQSLTDRVIREDECRKLTGVSRTTRYELEKKGGFPSRR
                     NLGGRSVGWLLSEVMDWVQSRDRVNSGKAA"
     gene            complement(17774..18628)
                     /locus_tag="PN977_001365"
     CDS             complement(17774..18628)
                     /locus_tag="PN977_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000163378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6041416.1"
                     /translation="MTKKVNSKKDLPKSFDLGKYDCLENLSDKDLFRQLYWRQDDLTM
                     KHSEMPEYGFMFGAEYPLHNNYGDPFGELKEDDWFCDKQKEYDHKVQPKLIELSYDDG
                     IKPVTRFDISMINKLTAERGYWKDKPIIIDNEMVGSLISEDNGMFWAVMREPVNLLSD
                     TLDNMLVSVDLLHNRDDELIEAFTKLLPKWRSELSIVEPDKPIAGSWESIRRKIIDYK
                     IIPLIDLLSWELSTDRKISLGVLAVSLYPDGEKDTFAIAQTVKPFLEKIMRSDSLEKI
                     RKMLSNEN"
     gene            complement(18625..19845)
                     /locus_tag="PN977_001366"
     CDS             complement(18625..19845)
                     /locus_tag="PN977_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000513577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDI6041417.1"
                     /translation="MGKALNKLSDSTLKKLAAVQAEKERFYSDGGGLEIKHSKGGKLT
                     WYFRYRTGGREVAAERLKLGAYPELSLKAAREKRTLCRAWLAEGKNPRYELCATVQEA
                     LKPVTVKEAINYWLEEYAKDNRKDYIKLVQRMDKHIICHIGAIPLDKCDTRQWIACFD
                     RVRKKAPVAAGHVMQTCKQALKFCRRRRYAFSNALDDLIVTDVGKRAEIRERVHSNSE
                     LKEILRAIDGDVFAPYYSALMRLLIVFGCRTAEIRLSEIKEWDLKEMLWTVPKEHSKT
                     KVTIFRPIPDGILPFIQKLVEQNAHTGLLLGELKKDTTVAQYGRNAHKRLKQEHWTLH
                     DFRHTFTTMLNDLGVDPHIVEHITAHQMPGQQKTYNHSRYLQAKRDALNLWVERLDMI
                     AGYNENIVILRGIQ"
     gene            complement(20175..20447)
                     /gene="rcnR"
                     /locus_tag="PN977_001367"
     CDS             complement(20175..20447)
                     /gene="rcnR"
                     /locus_tag="PN977_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II)-binding transcriptional repressor
                     RcnR"
                     /protein_id="HDI6041418.1"
                     /translation="MSHTIRDKQKLKARASKIQGQVVALKKMLDEPHECAAVLQQIAA
                     IRGAVNGLMREVIKGHLTEHIVHQGDELKREEDLDVVLKVLDSYIK"
     gene            20568..21392
                     /gene="rcnA"
                     /locus_tag="PN977_001368"
     CDS             20568..21392
                     /gene="rcnA"
                     /locus_tag="PN977_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel/cobalt efflux protein RcnA"
                     /protein_id="HDI6041419.1"
                     /translation="MTEFTTLLQQGNAWFFIPSAILLGALHGLEPGHSKTMMAAFIIA
                     IKGTIKQAVMLGLAATISHTAVVWLIAFGGMVISKRFTAQSAEPWLQLISAVIIISTA
                     FWMFWRTWRGERNWLENMHEHDHEHHHHDHEDHHDHGHHHHHEHGEYQDAHARAHAND
                     IKRRFDGREVTNWQILLFGLTGGLIPCPAAITVLLICIQLKALTLGATLVVSFSLGLA
                     LTLVTVGVGAAISVQQVAKRWSGFNTLAKRAPYFSSLLIGLVGVYMGVHGFMGIMR"
     gene            21611..21949
                     /gene="rcnB"
                     /locus_tag="PN977_001369"
     CDS             21611..21949
                     /gene="rcnB"
                     /locus_tag="PN977_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416610.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II) efflux transporter accessory
                     subunit RcnB"
                     /protein_id="HDI6041420.1"
                     /translation="MTIKNKMLLGALLLVTSAAWAAPATAGSTNTSGISKYELSSFIA
                     DFKHFKPGDTVPEMYRTDEYNIKQWQLRNLPAPDAGTHWTYMGGAYVLISDTDGKIIK
                     AYDGEIFYHR"
     gene            complement(22031..23065)
                     /locus_tag="PN977_001370"
     CDS             complement(22031..23065)
                     /locus_tag="PN977_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative fimbrial-like adhesin protein"
                     /protein_id="HDI6041421.1"
                     /translation="MEIRIMLFIVMMMVMPVSYAACYSELSVQHNLVVQGDFALTQTQ
                     MATYEHNFNDSSCVSTNTITPMSPSDIIVGLYNDTIKLNLHFEWTNKNNITLSNTQTS
                     FTSGYSVTVTPAASNAKVNVSAGGGGSVMINGVATLSSASSSTRGSAAVQFLLCLFGG
                     KSWDACVNSYRNALAQNAGVYSFNLTLSYNPITTTCKPDDLLITLESIPVSQLPATGN
                     KATINSNKGDIVLRCKNLLGQQNQTSRKMQVYLSSSDLLTNSNTILKGAEDNGVGFIL
                     ESNGTPVTLLNITNSSKGYTNLKEIAAKSKLTDTTVSIPITASYYVYDTNKVKSGALE
                     ATALINVKYD"
     gene            complement(23081..25561)
                     /locus_tag="PN977_001371"
     CDS             complement(23081..25561)
                     /locus_tag="PN977_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HDI6041422.1"
                     /translation="MLRMTPLASAIVALLLGIEAYAAEETFDTHFMIGGMKDQQVANI
                     RLDDNQPLPGQYDIDIYVNKQWRGKYEIIVKDNPQETCLSREIIKRLGINTDNFASGK
                     QCLTFEQLVQGGSYTWDIGVFRLDFSVPQAWVEELESGYVPPENWERGINAFYTSYYV
                     SQYYSDYKASGNSKSTYVRFNSGLNLLGWQLHSDASFSKTNSNPGVWKSNTLYLERGF
                     AQLLGTLRVGDMYTSSDIFDSVRFSGVRLFRDMQMLPNSKQNFTPRVQGIAQSNALVT
                     IEQNGFVVYQKEVPPGPFAITDLQLAGGGADLDVSVKEADGSVTTYLVPYAAVPNMLQ
                     PGVSKYDFAAGRSHIEGASKQSDFVQAGYQYGFNNLLTLYGGSMVANNYYAFTLGTGW
                     NTRIGAISVDATKSHSKQDNGDVFDGQSYQIAYNKFVSQTSTRFGLAAWRYSSRDYRT
                     FNDHVWANNKDNYRRDENDIYDIADYYQNDFGRKNSFSANMSQSLPEGWGSVSLSTLW
                     RDYWGRSGSSKDYQLSYSNNLRRISYTLAASHAYDENHHEEKRFNIFISIPFDWGDDV
                     TTPRRQIYMSNSTTFDDQGVASNNTGLSGTVGSRDQFNYGVNLSYQYQGNETTAGANL
                     TWNAPVATVNGSYSQSSAYRQAGASVSGGIVAWSGGVNLANRLSETFAVMNAPGIKDA
                     YVNGQKYRTTNRNGVVVYDGMTPYRENYLMLDVSQSDSEAELRGNRKIAAPYRGAVVL
                     VNFDTDQRKPWFIKALRADGQPLTFGYEVNDIHGHNIGVVGQGSQLFIRTNEVPPSVN
                     VAIDKQQGLSCTITFGKEIDESRNYICQ"
     gene            complement(25577..26251)
                     /locus_tag="PN977_001372"
     CDS             complement(25577..26251)
                     /locus_tag="PN977_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HDI6041423.1"
                     /translation="MKGLFALLISSMVLPAHAGIVIYGTRIIYPAENKEVMVQLMNQG
                     NRSSLLQAWIDDVDTSLPPEKIQVPFMLTPPVAKIGANSGQQVKIKIMPNKLPTNKES
                     IFYLNVLDIPPNSPEQEGKNALKFAMQNRIKLFYRPAGIAPVNKATFKKLRVNRSGNG
                     LVIKNDSANWVTISDVKANNVKVNYETIMIAPLESQSINVKSNNANNWHLTIIDDHGN
                     YISDKI"
     gene            complement(26332..26874)
                     /locus_tag="PN977_001373"
     CDS             complement(26332..26874)
                     /locus_tag="PN977_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HDI6041424.1"
                     /translation="MKRSIIAAAVFSSFFMSAGVFAADVDTGTLTIKGNIAESPCKFE
                     AGGDSVSINMPTVPTTVFEGKAKYSTYDDAVGVTSSMLKISCPKEVAGVKLSLITNDK
                     ITGNDKAIASSNDTVGYYLYLGDNSDVLDVSAPFNIESYKTAEGQYAIPFKAKYLKLT
                     DNSVQSGDVLSSLVMRVAQD"
     gene            complement(27170..27451)
                     /locus_tag="PN977_001374"
     CDS             complement(27170..27451)
                     /locus_tag="PN977_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehE family protein"
                     /protein_id="HDI6041425.1"
                     /translation="MNKYWLSGIIFLAYGMASPAFSSQTATLTINGRISPPTCSMAMV
                     NGQPQQHCGQLTYDVDTRHQVSSPVKGVTTEVVVAGSDSKRRIVLNRYD"
     gene            complement(27714..28823)
                     /gene="apbC"
                     /locus_tag="PN977_001375"
     CDS             complement(27714..28823)
                     /gene="apbC"
                     /locus_tag="PN977_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HDI6041426.1"
                     /translation="MNEQSQAKSPEALRAMVAGTLANFQHPTLKHNLTTLKALHHVAW
                     MDDTLHVELVMPFVWHSAFEELKEQCSAELLRITGAKAIDWKLSHNIATLKRVKNQPG
                     INGVKNIIAVSSGKGGVGKSSTAVNLALALAAEGAKVGILDADIYGPSIPTMLGAENQ
                     RPTSPDGTHMAPIMSHGLATNSIGYLVTDDNAMVWRGPMASKALMQMLQETLWPDLDY
                     LVLDMPPGTGDIQLTLAQNIPVTGAVVVTTPQDIALIDAKKGIVMFEKVEVPVLGIVE
                     NMSVHICSNCGHHEPIFGTGGAEKLAEKYHTQLLGQMPLHISLREDLDKGTPTVISRP
                     ESEFTAIYRQLADRVAAQLYWQGEVIPGEISFRAV"
     gene            28955..30988
                     /gene="metG"
                     /locus_tag="PN977_001376"
     CDS             28955..30988
                     /gene="metG"
                     /locus_tag="PN977_001376"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HDI6041427.1"
                     /translation="MTQVAKKILVTCALPYANGSIHLGHMLEHIQADVWVRYQRMRGH
                     EVNFICADDAHGTPIMLKAQQLGITPEQMIGEMSQEHQTDFAGFNISYDNYHSTHSEE
                     NRQLSELIYSRLKENGFIKNRTISQLYDPEKGMFLPDRFVKGTCPKCKSPDQYGDNCE
                     VCGATYSPTELIEPKSVVSGATPVMRDSEHFFFDLPSFSEMLQAWTRSGALQEQVANK
                     MQEWFESGLQQWDISRDAPYFGFEIPNAPGKYFYVWLDAPIGYMGSFKNLCDKRGDSV
                     SFDEYWKKDSTAELYHFIGKDIVYFHSLFWPAMLEGSNFRKPTNLFVHGYVTVNGAKM
                     SKSRGTFIKASTWLNHFDADSLRYYYTAKLSSRIDDIDLNLEDFVQRVNADIVNKVVN
                     LASRNAGFINKRFDGVLASELADPQLYKTFTDAAEVIGEAWESREFGKAVREIMALAD
                     LANRYVDEQAPWVVAKQEGRDADLQAICSMGINLFRVLMTYLKPVLPKLTERAEAFLN
                     TELTWDGIQQPLLGHKVNPFKALYNRIDMKQVEALVEASKEEVKAAAAPVTGPLADDP
                     IQETITFDDFAKVDLRVALIENAEFVEGSDKLLRLTLDLGGEKRNVFSGIRSAYPDPQ
                     ALIGRHTIMVANLAPRKMRFGISEGMVMAAGPGGKDIFLLSPDAGAKPGHQVK"
     gene            31129..34917
                     /locus_tag="PN977_001377"
     CDS             31129..34917
                     /locus_tag="PN977_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001215568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WGR and DUF4132 domain-containing protein"
                     /protein_id="HDI6041428.1"
                     /translation="MRHFIFQDEKSHKFWAVEQQGNELHINWGKVGTNGQSQVKSFAD
                     AAAAEKAGLKLIAEKVKKGYVEQAKDNSLQPSQTVTDSLKVANLSTIIQEQPSFVAET
                     RAADKNTDAVLPWLAKDIAVVFPPEVVHTTLSHRRFPGVPVQQADKLTQLRRLACGVS
                     QRDNKTATFDFSACTLEWQNFIAQAIDLINSPKTTKLPSPVMAVLAALELHCTRYKER
                     EDVMDQIIQEGGLEYATDVIIHLQQISIEWDYVNNNIVFLSSGISPDYLQQYSSFELR
                     LRKHLSLAEESLWQKCAQKLIAAVPHIPEWRQPLIALLLPEKPEIAHEIAQRLLGQKK
                     LPSLEWLKIVATDEHILASLEKYHEPYAIFDDYYCGAIWSATVLQEQGVAALPRFAPY
                     VASDYCADVLRHINHPFALTLLIRVAGHTKRCHDRMTKACAAFPHAALAALAELLVQK
                     EENSWRIMLMTMLISQPTLAEQVIPWLSTPAVAVLKSCQQQLKQPSNHASADLLPAIV
                     VSPPWLSKKKKSPIPVLDLAPLNLESICTITDTEAKEFQTHWDWEPHKPGEGAKDFLY
                     SLGYRRWDFDTYKYIGASDSAIDAWEREDFATLIQMFKAHHAPYQGEWHLNSLPFLPM
                     QKAIKLWEFLSKEPHTAIKPVMLYLRLAGMSGFLHSFSRYPQEGFAVANYFAATELAP
                     AVARAFNKLKTLRQDARTWLLKYPQHAITGLLPAALGKAGESQDNARAALRMLIENGY
                     PSLLQEIAQRYNQPEVTDAVNALLALDPLDNHPTKIPTLPAFYQPSLWTRPVLKANAQ
                     SLPDSALFHLGEMLRFPQEDTLYPGLLQVKDACTADSLAEFAWDLFTAWQTAGVPSKE
                     SWAFTALGVLGNDDTARKLTPLIRAWPGESQHKRATVGLDILAAIGSDIALMQLNGIA
                     QKLKFKALQERAKEKIADIAESRELTVAELEDRLAPDLGLDDNGSLLLDFGPRQFTVS
                     FDETLKPFVRDASGSRLKDLPKPNKSDDETQANDAVNRYKLLKKDARTVAAQQVARLE
                     SAMCLRRRWSPENFQLFLVEHPLVRHLTRRLIWGVYSTENELLACFRVAEDNSYSTAD
                     DDLFTLPEGDISVGIPHVLEISPTDAAAFGQLFADYELLPPFRQLDRNSYALTEAERN
                     ASELTRWAGRKCPSGRVMGLANKGWIKGTPQDGGWIGWMIKPLGRWSLIMEINEGFAV
                     GMSPAELSAEQLLSKLWLWEGKAESYGWGSNSTQEAQFSVLDAITASELINDIEALFE
                     "
     gene            34927..38589
                     /locus_tag="PN977_001378"
     CDS             34927..38589
                     /locus_tag="PN977_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4132 domain-containing protein"
                     /protein_id="HDI6041429.1"
                     /translation="MDKELPWLADNAQLELKYKKGKTPLSHRNWPGEPVPVITESIIQ
                     TLGDELLHIAEKKKNIVWRYDNFSLEWQSAITQAINLIGEHKPSIPARTMAALACIAQ
                     NDSQQLLDEIVQQEGLEYATEVVIARQFIVRCYESDPLVVTLQYQKEDYGYGYGYGYG
                     YGYGYRSETYNEFNLRLRKHLSLAEESCWQRCADKLIAALPGLPQVRRPFIALILPEK
                     PEIANELVGLECPWTHFHSKEWLKVVATDHRAVGKLERYWSQDIFSDREASYMSHENH
                     FGYAACAALLREQGIAAVPRLAMYAHKEDCGSLLVQINHPQVIRTLLLVADKNKPSLQ
                     RVAKYSKNFPHATLAALAELLALKEPPARPGYPIIEDKKLPAQQKARDEYWRTLLQTL
                     MASQPQLAEEVMPWLSTQAQAVLNSYLSAPPKTVIDSTDNIQMPEILVSPPWRSKKKM
                     TVPRLDLAPFELTPQVYWQPGERERLAATESARYFSTESLAERMEQKSGRVVLQELGF
                     GDDVWLFLNYILPGKLDAARNSLIVQWHYYPGRVEEIMNGWSSPEAQLAEQALRNGHV
                     EVLINIWENDSYSRYRREKSIWNLYLLAQLPREMALTFWLRINEKKHLSAGEDYFLSI
                     FGLDALPGLLLAFSHRPKETFPLILNFGATELALPVARVWRRFAAQRDLARQWILHWP
                     EHTATALIPLVFTKSSDNSEAALLALRLLYEQGHGELLQTVANRWQHTDVWPALEQLL
                     KQGPMDIYPARIPKAPDFWHPTMWSRPRLITNNQPVTDDALEIIGDMLRFTQGGRFYC
                     GLEQLKTFCQPQTLAAFAWDLFTAWQQVGAPAKDNWAFLALSLFGDESTARDLTTQIL
                     AWPQEGKSARAVSGLNILTQMNNDMALIQLHHISQRAKSRPLRDNAAEFLQVVAENRR
                     LSQEQLADRLVPTLGLDDPQALSFDFGPRQFTVRFDENLNPVIFDQQNVRQKSVPRLR
                     ADDDQLKAPEALARLKGLKKDATQVSKNLLPRLETALRTTRRWSLADFHTLFVNHPFT
                     RLVTQRLIWGVYPANEPRRLLNAFRVAAEGEFCNAQDEPIDLPADALIGIAHPLEMTA
                     EMRSEFAQLFADYEIMPPFRQLTRRTVLLTPDESASNSLTRWEGKSATVGQLMGMRYK
                     GWESGYEDAFVYDLGEYRLVLKFSSGFNHYNVDSKALMSFRSLHLYRDNKSVTFAELD
                     VFDLSEALSAPDVIFH"
     gene            38650..38970
                     /locus_tag="PN977_001379"
     CDS             38650..38970
                     /locus_tag="PN977_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6041430.1"
                     /translation="MIVQKELVAIYDYEVPVPEDPFSFRLEIHKCSELFTGSIYRLER
                     FRLHPTFHQRDREDIDPLINDALIYIRDEFIDERKLRGESPEMVIAIFNRELQNIFNQ
                     EIEK"
     gene            39546..40634
                     /locus_tag="PN977_001380"
     CDS             39546..40634
                     /locus_tag="PN977_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HDI6041431.1"
                     /translation="MSPQNNHLQRPPAAVLYADELAKLKQNDHAPCPPGWQLSLPAAR
                     AFILGDSAQNISRKVVISPSAVERMLVTLATGRGLMLVGEPGTAKSLLSELLATAISG
                     DAGLTIQGGASTTEDQIKYGWNYALLINHGPSTEALVPAPLYQGMRDGKIVRFEEITR
                     TPLEVQDCLLGMLSDRVMTVPELTGEASQLYAREGFNIIATANTRDRGVNEMSAALKR
                     RFDFETVFPIMDFAQELELVASASAHLLAHSGIPHKVPDAVLELLVRTFRDLRANGEK
                     KTSMDTLTAIMSTAEAVNVAHAVGVRAWFLANRAGEPADLVDCIAGTIVKDNEEDRAR
                     LRRYFEQRVATHKEAHWQAYYQARHRLP"
     gene            40645..42924
                     /locus_tag="PN977_001381"
     CDS             40645..42924
                     /locus_tag="PN977_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6041432.1"
                     /translation="MSEPLIVGIRHHSPACARLVKSLIESQRPRYVLIEGPADFNDRV
                     DELLLAHQLPVAIYSYCQYQDGAAPGRGAWTPFAEFSPEWQALQAARRIQAQTYFIDL
                     PCWAQSEEEDDSPDMQEESQALLLRATRMDNSDTLWDHLFEDESQQTILPSALAHYFA
                     QLRGDASGDALNRQREAFMARWIAWAMQQNNGDVLVVCGGWHAPALAKMWRECPQEIN
                     TPELLLLDDAVTGCYLTPYSEKRLDVLAGYLSGMPAPVWQNWCWRWGLQQAGEQLLKT
                     VLTRLRQHHLPASTADMAAAHLHAMALAQLRGHTLPLRTDWLDAIAGSLIKEALNAPL
                     PWSYRGVIHPDTDPILLTLIDTLAGDGFGKLAPSTPQPPLPKDVTCELERTAISLPAE
                     LTLNRFNPNGLAQSQVLHRLAILEIPGIVRQQGSTLTLAGNGEERWKLTRPLSQHAAL
                     IEAACFGATLQEAARHKLEADMLDAGGIGSITTCLSQAALAGLASFSQQLLEQLTLLI
                     AQENQFAEMGQALEVLYALWRLDEISGMQGAQILQTTLCAAIDRTLWLCESNGRPDEK
                     EFHAHLHSWQALCHILRDLHSGVNLPGVSLSAAVALLERRSQAIHAPALDRGAALGAL
                     MRLEHPNASAEAALTMLAQLSPAQSGEALHGLLALARHQLACQPAFIAGFSSHLNQLS
                     DADFINALPDLRAAMAWLPPRERGTLAHQVLEHYQLAQLPISALQMPLHCPPQAIAHH
                     QQLEQQALASLQHWGVFHV"
     gene            42917..>43206
                     /locus_tag="PN977_001382"
     CDS             42917..>43206
                     /locus_tag="PN977_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6041433.1"
                     /translation="MSELNDLLTTRELQRWRLILGEAAETTLCGLDDNARQIDHALEW
                     LYGRDPERLQRGERSGGLGGSNLTPPEWINSIHTLFPQQVIERLESDAVLRYG"
BASE COUNT        10744 a        11248 c        10040 g        11174 t
ORIGIN      
        1 cagcgatgcg cgtctcgcca ttaggttgtt tgcttccggc gcgcgatatt gactctttta
       61 ttgatgatgt ggcgctggcc tccagcccga cacataaatc cgatctggcg gttgcggggg
      121 cggtagtcat cgcatgggcg atttctcgtg ccattgacgg agaaagctgg tcagcgattg
      181 ttgattcact gccttcaatt gcgcgacatg ctcaacaaaa acgcatcact accttcagcg
      241 cctcactggc agcacgtctg gagattgcgc tgaaaattgt gcgcaatgcc gatggcaccg
      301 aatccgccag cgaacagctt taccaggtcg ttggcgcagg taccagcact attgagtccg
      361 ttccgtgcgc cattgcgctg gttgaactgg cacaaaccga cccgaaccgt tgcgccgtcc
      421 tgtgcgctaa ccttggcggc gacacagaca ccatcggtgc tatggcgacg gcaatttgtg
      481 gcgcgttgca tggcgttaac gctatcgatc ctgcgttaaa ggcggaactg gatgcagtaa
      541 atcagcttga tttcaaccgc tatgccacgg cgctggcgaa gtatcgccaa caacgggagg
      601 cgatatgagc ggcgctcgat tacacacgct gctgcctgaa ttaaccacgc gtcagccggt
      661 gatggtcgtc ggcgcggcgg tcattgatgt gatcgccgac gcttatgccc tcccctggcg
      721 tgggtgcgat atcgaactga aacagcagag cgttaacgtt ggcggctgcg cactgaatat
      781 tgccgtggcg ttaaagcgcc tcggcatcga agcgggtaat gccttgccgc tcggtcaggg
      841 cgtgtgggcg gagattattc gcaaccggat ggcaaaagag ggcttaatca gcctgatcga
      901 taacgccgaa ggtgataacg gctggtgtct ggcgctggtt gagcctgatg gcgaacgcac
      961 ttttatgtca ttcagcggtg tggaaaatca gtggaatcgc cagtggctgg cgcgattaac
     1021 cgttgcgcct ggcagcctgc tctatttttc cggttatcaa ctggcctcgc cctgcggcga
     1081 attgttagtg gaatggctgg aagagctgca agacgtgacg ccatttatcg attttggccc
     1141 acgtattggc gatatcccgg atgcattact ggcgcggatc atggcctgtc gacctttagt
     1201 gtcactcaat cgtcaagagg ctgagattgc cgccgaacgt tttgctttat ccgcagagat
     1261 aacaacacta ggcaagcaat ggcaggagaa atttgccgcg ccgttgatcg ttcgtctcga
     1321 taaagagggc gcatggtatt tcagcaacga cgcttctggc tgcattccgg catttccaac
     1381 gcaagttgta gacaccattg gggcgggcga cagtcatgcc ggcggcgtac ttgccgggct
     1441 ggcctctggt ctgccactgg cggatgccgt attactgggc aatgcagtgg cgtcgttggt
     1501 tgtcgggcat cggggcggtg attgtgcgcc aacgcgcgag gaactactcc tcgcacacga
     1561 aaacgtatag atcgctgcga cagtggctga tgctgtactc aataggccgt tgctgttggt
     1621 caagcgccac ttgcttgatc accagcaccg gtattttgct atctaactga atatgtgact
     1681 gaaactcggc atccggcatc cgggcgctaa cgcgggaacg cgtacgttgt gggtaaatgt
     1741 gctggctgcg gaagtagtca taaagagaaa tcccgatggc atcaacatca tgaattaaat
     1801 gtgccggaac ccacgattcc tcaatcgata ccgcttcttc atcgacatag cgaatacgct
     1861 taagcaaaaa gacatcactt cccgcttcga ccgccagctg ctgcgcaact tcttcgggac
     1921 atttcacgaa acgcttgtta acccatagcg tatcgggctt tttcccgcgc aataccacct
     1981 gctgagaaaa acctcgcgct tctttcaacg agtattcgaa gatgttgttg atctgcgtac
     2041 cgtaaccacg cgaacgcgtc accacgccct cttcttccag cgcctgcatc gccttgcgca
     2101 ccgtaatgcg cgaaacgccg gttaactgac ttaaatcacg ctcaccgggc aaaatattgc
     2161 catgctccag caccccgctg cgcacggcat tttttaccgt ttcggcaaac ttaatataaa
     2221 gcggcgtgtt atccgccgct aaaatacgtt cgttcagttg ggcaatcagc tgggtatgcg
     2281 cttgttccat ttgtcttttt cctgccgggg agtctggtgg gagtatatca ctgcttctta
     2341 tttaaggcgt ttctttaatc ccatcaacga atccctgcaa ctcctcctct gtgccattaa
     2401 acacattaaa atccaccgga ccattaatgc catctacctg cccacggtcg gaatgctgcc
     2461 agaagcgcca ggccatgccg tcattgtccg gacgacgttg atagtagtga gccacccacc
     2521 acggatattc attgaaatag cccgccagat tcgtgtgata aaaaacggct cctgagtaaa
     2581 taatcggctt ttttcccgtg cgtttttcga ccatttttag ccactgactt acccgcttgc
     2641 gtaattcttt tgccgataat tttccccgtt cttctacgtc cagcacggca ggtaaatcgc
     2701 cttgtgagaa atccaccgtt tgcagaaata atcgcgcctg aactgaagcg gataccgacg
     2761 gggagaaata atgatacgcc ccgcgcagca ggccattttc gcggcttagt tgccagttac
     2821 gcgaaaaata ggggtccacc agcttttcgc cttccgtcgc cttaataaag gcaaattgta
     2881 agcgaatacc gttatcgcgc atttttgcca cccgctgcca gtcgattcgc tcctgccagc
     2941 gtgaaacatc aataccatga atggtgtaac tggcaggaat gcgaatagca aaagatttga
     3001 ccgggcgata accgtagaag tggatgtagt ctttaatctg aattgctgtc gaatagaaaa
     3061 aattcaccgt ctgacgcgga taaattgcaa cgatggaaat tagcccaaga gcgcataata
     3121 aaacggtgaa tttttttcga ctggtgattc tcagctgcat tggtttcatc cctgaatgtc
     3181 agcgccaaag gctgacaata accaaagcag ctatagtacg gtgctcacca ccaagcgtgg
     3241 aagtggtgaa ccggaccaat accgtgacca acttccagcg tgtcggcctg ggctaacgcc
     3301 gatgaaagcc agctttttgc ctcctgtacg gtgtcagccc agtttgtatg gcgcgggcgc
     3361 agtgcagcca acgccgcaga gagcgtacaa ccagtgccgt gagtgttttt ggtcataatg
     3421 cgcggtgcgg taaaccgttg ttcaccctcg cgggtaaaca gccagtccgg gctttgctca
     3481 tcatccagat gaccaccttt cattagcact gcgccacagc ccatcgccaa cagcgatcgc
     3541 ccttgttcca gcatttcctg ttcggtgcgc gcgtgtggcg cgtcgagcaa ggcggctgct
     3601 tcgggcaagt ttggcgttat taatgaaacc tgtggcaata atcgactgcg cagcgtagca
     3661 accgccgaag gtgaaagcag cgggtcgccg ctttttgcca gcataacggt gtcgagtacc
     3721 acgttttgga tctgataacg ttgcaaccgt tctgccaccg cttcaacaat atcagtttcc
     3781 gccaacatac cgattttagt ggtgtcgatt cgcacatcgc tgaacaccga atcgagctgg
     3841 gcggcgacaa aatcaggctc aatgcgatac accgactgta cgccacgggt attttgcgcc
     3901 accagtgcag taatcactga gcaaccataa gcgccaagtg ccgagaaggt tttaagatcg
     3961 gcctgaatcc ccgcaccacc actcggatca gtaccggcaa tcgtcagagc gttaattcgt
     4021 ttcatgcctg cacctcctgc gtcaattgcc agagcgcatc aaggaaatgt ggaacaaaac
     4081 tgcctggccc ctcgcttctg gcgactgcgc gttctccggc ttgtttcatc cagtgacagg
     4141 caaatgcgat attttccagc gtatcgcctg gtagcgcaca acaggcagcg acaaccgccg
     4201 atagtgcaca gccagttcct accactttgg tcattagcgg atcgccaccg tgaatgccaa
     4261 cgatacgatg tccatcggta acataatcca cctcgccagt gaccacgacg attgcgccag
     4321 tttcccgtgc cagtgtttgt gcagcaggta tcgcgttagc tgcggcatca gtggtatcca
     4381 ctccccgtcc gccattagca acgccagcta atgccatgat ttccgaagca ttaccacgta
     4441 tggcggttgg cttatgagac agaagttcca gacaaaaacg gcggcgataa tcgagtgcac
     4501 ccaccgctac tggatcaagc gtccagggtg tttgagagcg ttttgcctgt tcaacggcgg
     4561 cacgcatcgc ctgtgcgcgt ggctgcgtca gcgtgccaac gttaatcagc aacgcactgg
     4621 cgattgccgc aaactgactg gcctcttcgg tttcgatgac catcgctggc gatgcaccga
     4681 gcgccagtaa ggtattggca gtaaaggttt gcaccacatc attggtcata cagtgcacaa
     4741 ggggggaatg tttgtgaaaa agatgtaacg cgtgcgcaga ttgcgcggaa ctcagcaggt
     4801 cgacttgcat agtttgctcc tgccataacg tgaagaagca atgacctggt ggtccgtgac
     4861 ttccctacgc tggcattatc cagatcaggt gatacgggta tttcttacta attcctgtta
     4921 gtaattgata ataaataagt ttttaagaat gcataaatta aaagcctcaa aactgaccac
     4981 acaactgacc acacatttcg ttgaagctct acgaacaacg atgaacaata acaaacaaaa
     5041 gggaaatttg ctggtggttt tgcgtaaccg ttatcatttt ccggctatcc gtcatgaagc
     5101 tggtggatga tctgcgtggt gatttccacc attcagagca ggggcggtca tcatgaccat
     5161 gccataacaa aaaccccata ttcgtgggtt ttctgtgttg tggctattct gccgttgccg
     5221 tgctggtgga tatttccggt atgtggctca ccatatcggc atcaggccca ccagcgtgaa
     5281 gcgtttttca tttttcgcgg gtataaaaaa tcgatggggc tggcagtccg gaagacgtca
     5341 gggtacagag atttgacccg cccctccctg tgcatgagca caataagtaa ccgaaggcgg
     5401 aattccgccc tcggtgctca catatgagaa acgatatttc ttacaccgat accctttatt
     5461 tgggtatcgc gagtaagccc caaaatctgg gtgttactct tactacccaa actacgggta
     5521 gtttcatagt cgagttgcag atctgcaact cgccaccagt tgctaagtgc atttttgcac
     5581 tttcggtaat atcaaccagt taccgccgta agtgctccgg cttcctccac tggtaagtat
     5641 ttttcgcgct ctccctccgt tgttgagaac ggcgacggta tgccagcaac tcaagaactc
     5701 tggttcgtat gttgcgcata tccacgccgt taagctcaat accgtcacgg cgcattacct
     5761 cagccaccac acgcgcgtaa ttttcggcta tcacgctgtc cggctgtgta gcctgttcgt
     5821 caggctgctg actgattccg gtaatgcggc gaattatttt tagtagatca gtttcagtca
     5881 ttctcgtcat accccatcaa atgccgcaag tcgctctttc tggctgtcgc tcatatcaaa
     5941 tgcaaattcc tcatgctcag cctggaatgt gccaaacgcc atcagcgccg caacgctcgg
     6001 gtctatctta ttggatgatt ttttcttgtt cggcttgata ttggcgttcg cgtcactctg
     6061 catcacaaca ttactcattg accaggccag caccggatca ccacgatgaa caatcacctt
     6121 ccggttaaca aaaacttcga acgatttcgc cgccggacta aaacgaaggt acgtttgcgg
     6181 gaacggctcc acctcaaatc ccgctccctg tagctgcgtc ctcaggtgcg tggcgttcca
     6241 tgtatcgaaa cccaccagcc tgatattgaa attctctgca tccgccatga tgtcatcacg
     6301 gatacggtca taatcaatgc agtcacccgg tgttgtgcgt atccagcccg cctttgccca
     6361 ctggcgatag atggcgcggt ttttattggc agggttctgt agctgaaatt ccggtagata
     6421 gtgacgtgaa accagcataa tctttttacc gaccggaaag gcatagcaca cgctggaaat
     6481 atcgctggtt gatgataagt ccagccccgc atagcactcc tgcccgtata aatccgcctc
     6541 agcgaacgtt ccggcgcact ccgcccatgc accgttaccc atccacggcg tagccccctg
     6601 acaccagata ttgaatcgct tggtgagcat ttccacccac tgcgacggaa taccccgcgc
     6661 tttctggatg gttgaggcca gtttttcacg atcgacggaa acatcgatat tgggattcgc
     6721 ctttatccac atcgccggat cgtcaacctc gctttcatca tccagctcgt aaatcagtac
     6781 gaacatggat tcgttcacct cttcaccatc cagtatctgg cagcaatagt cgtagtgttg
     6841 tttacaggct gaaacaacgt tgctccccga tgtggtgatg gcaaataaca gcccctccgg
     6901 acgcgccccc atccccagtt caagtgcgga ataaaccccg ttgtctgggt gcaggtgata
     6961 ttcatccaca atggcaagac tcgggtttgt cccctcaatg gttgccgctt ttgctgccag
     7021 tggctttaac aggctgttgg ttttcgggtg tatcaccttg tgcgcctgaa tatttacccg
     7081 ctttcgtaac ggtcgggata aaaggcacat ctgacgcgca tcatcaaaca cgatccgcgc
     7141 ctgatcacga ctcacggcgg cggtgtaaat atcctgctgc ccgttttcca taaccagaaa
     7201 ccagttagcc aggatagcgg cgaccgtgga tttggcattt tttcgcggta cttcaatgaa
     7261 tgcgctggtg tatttgcgcc gtccggtggc cttaacctta aatcccagga tgcaggcaaa
     7321 ggcgaactgc tgccacggct caagttcaat ggggctaccg cgcattgcgc cttttacgtg
     7381 cgggcacacc cgggaaaagg caataaaccg ctccacgacc tccggatcga acgtgtaaag
     7441 ggagttttca aggtcagaaa aataccgttt aacagcctgt ttcagtcgtt tacaggccgt
     7501 aattttgccg ttttttacgc cttctgcgta ctcatgccag gcggtcaagt tcgtcctctt
     7561 cctctgtttc cggtggattt ctgcgacggc ttaccgggtc aaaacccagc aaagaagcca
     7621 ttttgatcat tattctttct gcgtcagcct ttgcgctcag tgcggggttt ctgctctcgc
     7681 tgccctgact gttaacaatg ctgaacccgc gcgtcgcaag gtcttcgacg gctttgcggt
     7741 atatagagta gttaacgcaa tacagttcca gattgctcca gtcggcgggg gtaaggtctt
     7801 cccgcccgga aagctggcgc gatttttcct tccactgcct gaccgcgatt tcatccaggt
     7861 aagcgggggc ttttggtggt cttgccatgt tcttttttcg cccaattatt ttcaaaaaaa
     7921 ttaccgtgca caaaaatttg agggggcggt cggtgtccgg cagggacggt ttcgtcctga
     7981 aaaccacccc caccccctct gacggcctca ccagcgattg cgaaaacatt ccatgacctc
     8041 gcggtcacgg tcggttaatc gcttcgctgt ggtgcgtttt gtgcgccctg tcctgttggc
     8101 tttgtgctct gtctcctgtg tcttccatgt gtcacgctgc cttatcagtc cacgtatcag
     8161 cctggtttgc tcctgttcag tcatcatcgc catacatcca gtcgttacgg tgtgccgccc
     8221 gttcttcctg ctcgcgatac atgcccgctt tacggtttgc tttcgtggct ggatcttctc
     8281 gtgtcgtctt acggttgtgg cacgtctggc acaatgcctg gtgattccac tcaggccaga
     8341 agagaacatc accgccgcca ttgatgggaa tgatgtgatc caccacaaga gctggcgtat
     8401 aaatcccctt agccagacaa cgcacgcata acgggttttt gctcaggtac agggcgcggt
     8461 atttgtccca ctgtcgggaa tacccgcgcg cgcggcggtg tccccgtctg gcatcctctg
     8521 cacgccatgc agcccgcctg tgctcttcac acttgccgga cttcacgcgc ttattacagc
     8581 caggctcagt gcatcgcctt aatggttgcc acggcatcag tacaccccta cgtcacgata
     8641 aactgaccag agcgcagaaa tagccatagg cagttccgag tgctccactg gtgaaaccgc
     8701 ttcccggttc tcgtacagga aagcgatgta catcaggcaa ccaacacgca ttgccggggt
     8761 aaattccagc ccgtcttcaa aacgtttccc gatatgcttc tggcaggctt ccagcgccgc
     8821 atcggtatac attttcagaa gttcgccttc accggataaa tcatcatcaa gtcgaagatg
     8881 tgccctgact tcatcaggtg taattctggc ttcactcatc ttttctccct ttaatttcca
     8941 cagtctgttt ccatgcctgg ctgaactcat caccaccttc acgcggcggc ataccctcac
     9001 gctcacgggc ttcgttcgga ttcatgatcc cgttcttaat ccctttctca tacgtggcat
     9061 aacgttcggt gggtgtggcg cgtaataaat cggctgaatc aaactcaacc agataacggg
     9121 taccaggtac gggagaagcc accagcaaag cggccttgat ttgctgttcg aagttcgcca
     9181 gccacgggcg cattgtcatg gtcagaaacg cgcggcttgc ctcgctgaaa ttgctgtagg
     9241 tgctgttgct gtattcctgc agaaaaatag gcgatacgtt gaacatgcgg gcgatgtctt
     9301 caatggagaa gcgacgggag gccagccatt ccgcatcctg gttactcatt cccagttgcc
     9361 tgtaatccat gcccccttca aggattggcg tttttccggc atttttcgcc cccttgtagc
     9421 gttccagagc atccaatgcc tgtttacctt tcacgccgtc cagccattcg cctgacgtga
     9481 taatccctgc cgccatcatg ccatctttca taatgctggc tccgtgacgc tgttgagcaa
     9541 ggccaagccc cagcgcctca cggcaaatcg tgacggggga acgccccaga aagccatcat
     9601 ccgaggcata gcggagatgc aaaacttctt cctgtaaata cgtgcgcacc gttcctgtac
     9661 agggttcggt gatggtatag cggtatttgt gtgcgcctgt gcgttccggt acaacacacc
     9721 ccggcgcata aggatgaagt gattttggct gcccgtcctg cccccactca ataaccgcat
     9781 aggcgttacc gttcagcagg cagtgacgca tcattgtgcg tttaaactgg taaggtgtct
     9841 ggcacgaatt aggctgctca ttcagcagaa tatctactgg gtgactgtcc agccattccc
     9901 gcgcctccct gcccttgtca ttacgtacca gatacagata acacggcatc gtggccaccg
     9961 cctcagcgat gacagaaacc gcattcatca ctgcaggcaa tgattcagcc gtcccggcag
    10021 aaacatattc tccggatccg gtattcggta cgccggacag cgccagaaaa tcatcaatgg
    10081 acaggttacg ctgctcgctt tttttacgac taaaaggcca ccacatatca caaccccgcc
    10141 agctcagacc agcgatgacg attatttcct gtcggacgta attcagggtg ctgtgcaaac
    10201 aacgaacggt gggcaatctc cacgccagat tctggataag caggcatcga cgttattgtg
    10261 atttcacgga gttcagcggc ggttacggta cgcagatacg gtttttgcgc gatactccac
    10321 tcctcgcata atgcgcgaaa accaaagctc attcctgtaa tgtcgccacg ctccaccagc
    10381 gtaagcacat cttttccaag ctgggtatcc ggcggtgtca gttcaaaacg taacccggtg
    10441 ttatcctcag tcagtaccag agtgccggat ttggtgcgcc ccagcagttg ggtatagtca
    10501 tgctcataca ggcagcgcac atcattaccc gtcgccagat agtcagcaaa agcccccggc
    10561 gtgaactgtt cgcggaattc gtcccagata atttctgaaa ggctgttcca gcgaacggca
    10621 taccccacca gctttttatt gctggcggtc agttcagatg tgcggatttc aaaatcggtg
    10681 tttttcatcg gtgtactcca taaagctgaa aaaggaggcc gcagcctcct ccttactcat
    10741 gactagccag ctttcatttc cagaattttg atggcgtttg aatccaccac accaccgccc
    10801 agatatttat ccgtgtggac cttgtagaat ccgggttcag taatgttgtc cggtcgggtg
    10861 cgaatccctg ttacatgatc aacgatgaaa taaccacgac tgaaatcgcc aaccgctagc
    10921 ggtgcttttc ctgcgccgat gtccggcatg gactccaggc agtaaacagg acgaccaagc
    10981 aacatatccg gcgcaccttc tttaaggctg tcgcgccaga tataatcgcc gttctcattt
    11041 ttcagcttct gtagtgtccc tgccgtgccc gagttcatca cccagacggc atttttgcgg
    11101 tatttcgctt tcagcttgta gagaatgtcg atcagttcgt ccgctgtaat ggcggttcca
    11161 cttgccgcta ctattttttc aatggtgcca aaagcgcggg ttttgtcact ggtcgccgca
    11221 cgggtgtaag ccatgaagcc tttcggcttg ccgttaccgt cgccattaac aaaatcatcc
    11281 tcttcggtgc tggcgaacgt gtcggcaatt tcggaggata accagcccag aatatccacc
    11341 tctgaaaaat ccaggatttc ctgcgtggtt ttcgggtagg cgtagatcgg attcaacttg
    11401 atggttacgc gttcaatttt cggggtgttg gtttcactgc gtgcgctgcc ttctgtgcct
    11461 cgtcctacag ttgcgccgcc agtggaaacc agtttctgaa actcatttga ttttgcggtc
    11521 ttcacggtcg cgatcacgcg cataacactg tcatcctgta gctggcgcat gacttcgcga
    11581 tcaagctcag gaattacggt atatccgcca tccctgccgc tgtcagtgct ggtggacagt
    11641 gatcgcacat ctccggttac gatgtagtta cgcaattcat cagatgataa tttctggatg
    11701 cccgttcctg gcttgctgcg ttcttcatca gccacagact cgaggcggga aatttctgtg
    11761 tcgagggcat cagcttttgc acgcagttca tcaaactgtt tgccttcttc agcgttcaga
    11821 ctacggtttt cactgtcggc tttttccagc agggatcgca tctggttttt cagggcgttt
    11881 ttttgctggc ggagttcgat taatttcttc atgaaggttt tctcgtattg gttaagattc
    11941 aggacgtgaa accaacacgg ggggagcgcc gcccgacact ctcggcatct cgcagatcaa
    12001 cccggcatcg cgcagggggt caggcggcat tgtggcggct cacgtctgag tgccacgctc
    12061 caacatatac ataaaaatca gtatgtaaac acctgtcagt cagaccgaac aaccacgaaa
    12121 gaacacgaac aaataattta caaaagaagt aaaccatggc ctattattgc tgtctctgac
    12181 tacaacaaag gacgcatcat gaatttaagc gcaatggtat atcctgatac tttcataatt
    12241 aatggcgaat cattcagagg taaaagaaac gcaaaagaaa acaaggtatt aattccatat
    12301 acaaatgaac cggaagtaac tattggtcaa cacattatcc agcgtgtcgg taaaaacgag
    12361 ataaatctaa aaattataga tatgaagttg cttcccaatg gaacccgcag acagggaacc
    12421 aatcatccaa atatgttgac tctctatatt gaaaatatca cgggaaatga acatatgacg
    12481 ccaactaaaa gtaatacatt taacattggt tcaattagtg gtgatcaagt gcagataggt
    12541 gagcataacc atatgctggt aaacatcagc atcactgaac tggttgaaaa agtcgctaaa
    12601 tcgggggatg tacaagccaa atcagtatta aagcagttac tggaaaacag taccgttgcc
    12661 agcattgttg gggctggcgc aagtgcccta ataggcttac tgtaaaaaaa tggcctggat
    12721 tttaccaggc ctttattatt ttaccgtttc acgggatctc gcattctgcg ccttattttc
    12781 cacagatatt cgatcatcgc ttccacctgc tcacggttgg ttgcgaaaat ttccccggtc
    12841 agtgagctac gcagaaaatc atgatgatcc acaacaaaca acgcatcgga agaaagcaga
    12901 cggcgatatt tttttgctgt catggtttcc agatcatcaa aatcatgaaa ctttttatgt
    12961 tgctggactt cttcaaatgt cactggaatg tcccctctgt tgtcgctaaa acggtatgtt
    13021 gtccccgtac ggatcatcgt gctgacccgt ctgctgtttt gccctgttca gtgcgtcagt
    13081 ggcctgcccc tgctggcctt ttttgccgcc cggtcgcgct gttcgcgcac tgatcacgct
    13141 gtctgcgata acctgccagc cctgccgcgt ttcgccgttc tggcctgtcc actggcttac
    13201 ctgcatgtta cccgccacgc tcaccagttc gcctttgtgg tgttttgcca gtgcgtcggc
    13261 ctgtctgcca aacgccagga cggataacca catcatcgcc gttccgtcat ctgcctggct
    13321 gcacggaagg gggaccgcca tactagccat cgtcatttgt gtacccttgc tggtggtctt
    13381 taactgcggg tcagccacca gccgcccgta agccgctatc tgtgctgtca tgctgtctgc
    13441 tctccggttt taacgttgat ggttgtcacc tgttccgctt cggcaatctc ccgttctgtc
    13501 agcgtggcaa agtttgcagc tgccgttgtc atgaatgcgc taatcagttc gggatgtgct
    13561 ttcgcatatc cttccccggc gttgcggtcg atgattttta tcgacaccct taaccagtgt
    13621 tccgtcaaat caagggcgtg cgattgtgat ttttttgtgt gcttcgctgt cataggcttt
    13681 atctcacagc agtaaattaa aatttttgcg ttttaaccct tcacctgttc accttttgat
    13741 attttccatt taaattcata ctgttaaggg gtgaacagtt tcacaaaaac tattcaccaa
    13801 ctgttcacca ctgttcaccc ttgaagctca ataaacaacc aaaaaggtga acagtgaata
    13861 gtttggtgaa cagttcataa ataactgttc accctataat atactgatat aaaagataat
    13921 tatgacaggc tgaacagtgg tgaacagtta ttcaataagt ttaatttttg ctatcgtcat
    13981 tagtgaccga tacacatgat ggcatccagt cttctgattc ctccgtcagt gtcacgtttg
    14041 aacgaaaacc gtgcttcgtt ttccgtttca tatactccct gccatattcc gccattgccc
    14101 ccggcatatc tttaccgaag cgcgtcagtg ttacaggttt accaaaccca tgtgccctca
    14161 tataagccag ataggcatga tagagatacc tgcgtgggct gaatggcaca atttcagcat
    14221 tacccactaa caggccatca cacattaccg acgccatgag atagccgcag aagtccacca
    14281 gcgaatcccc ctctcgcttt atcgccagtg cttcttcaga tttctgctgc tcatataaca
    14341 ggcgtctggc ttcgtcctgg tcagcaaacc gtgtaagcag atggcgaatc actaccgcca
    14401 gctcaccttc tattttttcc gccagcattg gatcgcgttc gttctccggt acaacttccg
    14461 aaaaattgaa tatcacccga cgacgtgaga tccccccgct gcggtcactg aatgacatgg
    14521 cgttattgtt aaccgccagc actactgccg gaatacgcgt tgagtagggg gctttgtgtt
    14581 tcgggtcaat tgccaccttg tcaccgcctg taatagcctt aatccctgcc ccatcaccag
    14641 cgtagcgggt catatccggc atgataatca gcgaaaagcc aaccactaac gcgcgttccc
    14701 ttgcatcttc cagcgccttc atgcttgccg atactgtgtt ggccttaccc gccagcatgg
    14761 tgcaaatctc cgccatcacg cttttaccac ttccccctgg acctgttacc tcaatgaata
    14821 actgccagtc gtaccggttc gccagcacca tgaataatgc agccagtacg cgatccgcct
    14881 tgcggtcatt ctcagccacc gaacggcgta accacttcca gaaattcggc gcatgtgttg
    14941 ccagcgtttc cccctctgct ggtgggctga aaggtaattc actggcaatt aacaaccagt
    15001 cgtttttgtt atgctcccga aaattacctg ttctggtatc aaataccccg ttactgaatc
    15061 caatcaggtt acgggctgta ttccccatta caggcaaact taacttcatg gtatcgaccg
    15121 ccgatttaat ggcgttctgc gaatagctga tctccgcatc aatgaaaatc tgtgccatag
    15181 ctcgctgtaa ttctttatcc tgtactggct cccatacaac gccgttgtaa tggtgaacag
    15241 tgtcagagtc agcatgaatc gccagttcac cgccataatg tgccaggaga acttcgccgc
    15301 gttgacttgc tcccatctgg ttaagcgcca gtgatgaagc gttatcgtct tttacccgct
    15361 cttttttctt tacaggcagt tcaactacct ttttcttttc cgcctgctct gcccgttcac
    15421 gttccagata ttcgcgccag ttctcccgtt tctggctgtg cattccttca gggtaataat
    15481 cagcatccct gacacctgcc actgccagtt tctgcccgat ggtattaaca agccccggac
    15541 gcaataaccc cgcctgatag agacgcacgc gatagcgtcc gtccggtacg atttgcaggt
    15601 tgtccagttc ggcaagttgt tgctctccaa gccagacagg tggcacgtta tcgccagcca
    15661 gtcgcccgtc ctgttcctgc cactgtttcg catgtgccca cgcatcacta cccgcaaaaa
    15721 tgatgacttc cgtcattttg tcacgcggct ggtgttttaa atttggcgct tttttcattt
    15781 ctgctctctc cacgcggcaa tcatgttttt tagttcctgt agttttttat caacatccat
    15841 acatgacaca tggttatttc tggaaagcgg gatttcccga ctgaatctgc taataaagat
    15901 ctccacgttc agcgaactaa gaaatgaata gccatcacgg ataaaataca cacggtcaaa
    15961 catcagttct tttaccgtta ctctgttacc gttcttatcc agataaatag cgccggggat
    16021 aatttggggg tgtgcataac cgctggcagt caagccagat aaatacgttc tcatgattat
    16081 ttatccccga tttgaatcag tattcgcttt ctttatagca tttaatgcat ctgtggcatt
    16141 ttcaatggtg caccgtaacg aaatatcaaa atgtccaagc attgccagta acaaaccgat
    16201 attacccata tcaatgcgca tggccttttc gtcatagtcc tcattttctg acgcatgcca
    16261 catcaggcta ccaattgacg caacagccat tgatatattg tcagtagccc catccgcagc
    16321 ggaataaacc tttttagcaa tatcatgctc acagttaaaa tgcggattaa tcaggtactg
    16381 gtaatttgac atatcaggca tggcaccccc cctgacgaat acgggcggcg aataccatca
    16441 cgcagccagc cggggattgc tggcgtgctt cctgttcgct gctggcctca atggtaatca
    16501 cgcgcggttg tgccgtgctc agggcgataa aacgccagat gtatttattc aggttgtacg
    16561 agtcccgccc ttgcgggtgt atggtatgat ttctcatagc tgcctcgata ctctcgttat
    16621 cgttggtggt tagacgcccc gtatgtgttt ccggcactgc ggggcgttgc tctttgtatt
    16681 tcaacaatcc tttcggtgtg tttcatgtta tgagcgcatg aaacacacgt caaggctttt
    16741 tgtatttctt tttttgtgta tactgaaaca caccgatgat taggagtttc agaaatggca
    16801 acggctaaca aaaacgcaaa atcacaactg acaactgtca gagtcccact agatgttatg
    16861 caagggatgg aatccgttaa gctggacggc gaaagcaatg ccggatttat cgtaaccgcc
    16921 atgcgcggag aaatagcccg ccgccaagca gaaggcagcg gagaaaatcc ccttgtgtcg
    16981 tcactggatg ccctggctaa ggtcgaacaa atcggcatca aagcagcgga agaaatcggg
    17041 cagcttgtca ccgttgcacg cgaagaactc cagcgtcgca agaccaaaga accagagtaa
    17101 tcaccatcag cgccgtggtg taaggtatta cggcgcattg ctatgcagga caacacaatg
    17161 accgataaag aattgaccaa aacattatca ccggcacgga aaagacggcg cagaaagata
    17221 gagcatgaat cagaaagatt cgcgccatgt gcttttgctc ttgagaaatt ccttaaagag
    17281 cacaggaaaa agctctcgtt gcaaaccttg gaacgaacca aatctgactg atcacattgc
    17341 ccaccagccg caaatgtggc attgttggtg atgcgttcaa gtttagtgtg tatccattgg
    17401 cgaccgcccc cggtcgtctt tgttttatat gtcatatgct cccctttacg ctgccttacc
    17461 tgaattaacg cggtcacggc tttgcaccca gtccataact tcggaaagca accagcctac
    17521 agaacgcccc ccaagattac ggcgagacgg aaagccccct ttcttctcca gttcgtagcg
    17581 tgtagtacgg ctgacgcctg ttaacttacg acattcatcc tcacgaatta cgcgatctgt
    17641 tagtgattga aattgtgtat tttgattcat aaaaacaccc tcgaacgtaa ccgaacaagg
    17701 gcatcttatt taccatagca gttggtgcaa tattggaaaa ataaacttta ttctaattta
    17761 tcgttctcca tgattaattt tcattagata acatttttct tattttttct aaagaatcgc
    17821 tgcgcattat tttttctaag aagggtttta cagtctgagc aatagcaaag gtgtcctttt
    17881 caccatcagg atacaaagat acggcaagca cccctaatga aatttttcta tctgtagaca
    17941 actcccaaga taataagtca attaatggga ttatcttata atcaataatt ttacgtcgta
    18001 tgctctccca acttcctgca attggtttgt ctggctcaac aattgaaagt tcactacgcc
    18061 attttggtaa tagttttgta aacgcttcaa tcaactcatc atctctgtta tgtagtaagt
    18121 caacagaaac aagcatatta tctaatgtat cagaaaggag attcactggt tcacgcatca
    18181 cagcccaaaa catcccatta tcttctgata ttaaacttcc aaccatctca ttatctatga
    18241 tgattggttt atccttccaa tatcctcttt ccgcagtcag tttattaatc attgatatat
    18301 caaaccttgt tacaggttta attccatcat cataacttag ctcaattaac ttcggttgca
    18361 ctttatggtc atattctttt tgtttatcac aaaaccaatc gtcttctttc agctctccaa
    18421 atggatcccc gtaattatta tgcaacgggt attcagcacc aaacataaat ccatattcag
    18481 gcatctcaga atgtttcatc gttaaatcat cttgtcgcca atataattgg cgaaatagat
    18541 ctttatccga caagttttct aggcaatcat atttgcctaa atcaaatgac tttggtaaat
    18601 cttttttact atttactttt ttcgtcattg tatccctctc aatatcacaa tattttcatt
    18661 atatcctgca atcatatcaa gacgctcaac ccatagattc agtgcgtccc gtttcgcctg
    18721 caaatagcgt gaatggttat aggttttttg ctgacctggc atctgatgcg ctgtgatgtg
    18781 ctccacgata tgcggatcga cacctaaatc attcagcata gttgtaaacg tgtgtcggaa
    18841 atcatgcagc gtccagtgtt cctgcttaag ccgcttatgc gcatttcgtc catattgcgc
    18901 caccgtggta tcttttttca gttcgccgag taataaccca gtgtgtgcgt tttgctccac
    18961 cagcttctga atgaacggca aaataccatc aggaataggt cggaatattg ttaccttcgt
    19021 tttgctgtgc tcttttggca ctgtccacaa catttctttc agatcccatt ctttgatctc
    19081 tgaaagtctg atctctgccg ttctgcaccc gaacacaatt aacaagcgca ttaacgcact
    19141 gtaataggga gcgaacacat caccatcaat agcgcgtaga atttctttta gttcgctgtt
    19201 gctgtgcact ctctcgcgga tttctgctct cttacccaca tcagtaacga tcaaatcgtc
    19261 cagggcgttg ctaaacgcgt agcgccgcct gcggcaaaac tttagcgcct gtttgcatgt
    19321 ctgcatgaca tggcccgctg ctactggtgc tttttttcgt acgcggtcaa aacatgcgat
    19381 ccactgcctt gtatcacact tatcaagggg aattgcccca atatggcaaa tgatgtgctt
    19441 atccatacgc tgcaccagct ttatgtaatc tttgcggtta tccttcgcgt attcctccag
    19501 ccagtagtta atcgcttcct tcaccgtaac gggttttagt gcttcctgta ctgtggcgca
    19561 caactcataa cgggggtttt taccctcagc cagccatgcc cggcacagtg tgcgtttttc
    19621 ccttgcggct ttcagcgaca attcagggta agcccccagc tttaaccgct ctgcggcaac
    19681 ctcacggcct cccgttcggt accggaaata ccaggttaat ttgccgccct ttgagtgttt
    19741 aatctccagc ccgcccccgt cggagtaaaa acgctctttt tctgcctgga cagccgccaa
    19801 tttttttaac gtcgaatcgc tcagtttgtt tagtgctttt cccactatca gatcctcttt
    19861 tcagtcctcc agcctcaaaa aactgaccac acaactgacc acacattttg ttgaagctct
    19921 acgaacaacg acgaacaaca acaaacaata aaacaggata atttattgtt tatcaaatac
    19981 atacataaac aaccacaagc aaccccgaac cacaaaaaac actaaatgag aaaatacggg
    20041 tatttctcag ccttcacgca gaagggcacc ccgagtcgtt tggttgcgat gataaatcgc
    20101 aaaggaggat ggtaatgtcc agcgcacgcg ttgtaaacga ggaaaaacgg cagttttaca
    20161 atcgcgttaa taaattattt gatatatgaa tccagcacct tcagaacgac atccagatct
    20221 tcttcacgtt ttagctcatc cccctggtga acgatgtgtt ccgtcagatg acctttaatc
    20281 acttcccgca tcagaccgtt taccgcgcca cggatagcag caatctgttg taaaactgca
    20341 gcgcattcgt gcggctcgtc gagcattttc ttgagcgcca cgacctggcc ctgaatctta
    20401 ctggcacgcg ctttcagttt ctgtttatca cggatggtat gagacatggc aacaccaggt
    20461 taacaagaat atgaaaaatc atagcactat taatctactg gggggtagta tcaggtactg
    20521 ggggggagta gaatcagatt gccgaattaa tactaagaat tattatcatg accgaattta
    20581 caactcttct tcagcaagga aacgcctggt tcttcatccc cagcgccatc ttacttggtg
    20641 cgcttcatgg cctggaaccg gggcactcaa aaacgatgat ggcggcgttt atcatcgcca
    20701 tcaaaggcac cattaaacag gcggtgatgc tcggactggc agcaactatt tcgcataccg
    20761 cagtggtctg gttaattgcc tttggcggga tggtgatcag caaacgcttt actgctcaat
    20821 cagcagaacc gtggctccag ttgatttccg cagtgatcat tattagcacc gcgttctgga
    20881 tgttctggcg tacctggcgc ggcgaacgca actggctgga gaatatgcac gagcatgatc
    20941 atgagcatca tcaccacgat cacgaagatc accacgacca tggacatcat caccatcacg
    21001 aacatggcga gtatcaggat gcccatgcac gagcccatgc caatgacatt aaacgacgct
    21061 ttgatggtag agaggtcacc aactggcaaa ttttgctatt tggcttaacc ggtggcctta
    21121 tcccctgccc ggcagcaatt accgtgctgt tgatttgcat tcagttgaaa gccctgacac
    21181 tgggcgcaac actggtcgtc agtttcagcc tcggcctggc gttaacgctt gtcaccgtag
    21241 gcgttggcgc agcaatcagc gttcagcagg tcgcaaaacg ctggagcgga tttaacactc
    21301 tcgctaaacg cgctccctat ttttccagtc tgttgattgg cttagttggt gtgtatatgg
    21361 gcgtacatgg cttcatgggc ataatgcgat aactcaatcc tttaggcttg catgttatct
    21421 tcagcccagg atgattctta aatcagctat ttccgctgac tctctgctcg ctaaggatgc
    21481 ttttagggca tcctttcatt tacacttttt acgaaatcat gggatcacta acaaaatatc
    21541 gcttgtcagt tatattgtat ggcaggaaag atatgcgact gatattacag atccccaaag
    21601 tggagagttt atgaccatta aaaataagat gttgctgggt gcgcttttgc tggttaccag
    21661 tgccgcctgg gccgcaccag ccaccgcggg ttcgaccaat acctcgggaa tttctaagta
    21721 tgagttaagc agtttcattg ctgactttaa gcatttcaaa ccaggtgaca ccgtaccaga
    21781 aatgtaccgt accgatgagt acaacattaa gcagtggcag ttacgtaacc tgcccgcgcc
    21841 tgatgccggg acgcactgga cctatatggg tggcgcgtac gtgttgatca gcgacaccga
    21901 cggtaaaatc attaaagcct acgacggtga gattttttat catcgctaaa aaaagccccc
    21961 tcggagagag ggggaaatgc aaacaccttg ttatttttta ttattagcca cttgctcgtc
    22021 ttgcttatta ttagtcgtat ttcacattga ttaatgcggt tgcctccagt gcgccagatt
    22081 taactttgtt tgtatcgtaa acgtagtaac tggccgttat cggaattgaa accgttgtat
    22141 ctgtaagttt tgactttgcc gcaatttctt ttaaatttgt atatccttta ctgctgttag
    22201 tgatatttaa aagtgtgact ggcgtaccat tactttcaag aataaatcct acgccattat
    22261 cttccgcacc tttcaggatc gtgttgctgt tggttaacaa gtcagaactt gataaataca
    22321 cctgcatttt ccgtgatgtt tgattttgtt gacctaataa gtttttacaa cgcagaacaa
    22381 tatccccttt gttactattt attgttgctt tgttacctgt agctggtaat tgtgaaacgg
    22441 gaatactctc taacgtaatt aataaatcgt ccggtttgca ggttgtggtt atcgggttgt
    22501 atgacaatgt cagattaaag gaatagacac ctgcattttg tgccaatgca tttctgtagc
    22561 tatttacaca tgcatcccat gacttgccac cgaataaaca cagtaaaaat tgtactgcag
    22621 cactcccgcg tgtcgatgat gaagcactgg ataatgtcgc aacaccatta atcatcactg
    22681 aaccgccgcc ccccgcagaa acattcactt ttgcattact ggccgcaggt gtcaccgtta
    22741 ccgaataacc actggtgaaa ctggtctgag tatttgacaa cgtgatgttg tttttattgg
    22801 tccattcaaa atgtaaattt aatttaatgg tatcgttata aagtccaaca ataatatccg
    22861 acgggctcat aggggtgata gtatttgtac tcacgcatga cgaatcatta aaattatgct
    22921 catatgtcgc catttgtgtt tgagtaagtg caaaatcccc ctgaacaacc aaattgtgct
    22981 gaacagataa ctcactataa catgccgcat agctcacagg cataaccatc atcattacta
    23041 taaatagcat tatgcgaatt tccatagaac cacctgacta ttactggcaa atataatttc
    23101 tactttcatc aatctcttta ccgaaggtga ttgtgcatga aagtccttgt tgcttatcaa
    23161 ttgctacatt aaccgatggc ggtacttcat tggtgcgaat aaataactgg cttccctggc
    23221 cgacaacacc aatattatga ccatggatat cattgacttc ataaccaaac gttaatggtt
    23281 gcccatccgc tcttaacgct tttataaacc atggcttgcg ctgatcggta tcaaaattaa
    23341 ccagtacaac cgcgccgcga taaggggcgg caattttccg gttgccacgt aattctgctt
    23401 cgctatcgct ttgtgacaca tccaacatca ggtaattttc ccgataaggt gtcattccgt
    23461 cgtataccac cactccatta cggtttgttg tgcgatattt ttgcccattg acataagcat
    23521 ctttaattcc tggcgcattc atcacagcaa acgtttcaga aagacggttt gccagattaa
    23581 cgccacccga ccaggcgaca atgccccctg aaacactggc tccagcctgt cgataagcac
    23641 tcgactgact ataactgcca ttcactgtcg caaccggcgc gttccaggtt aaattcgccc
    23701 cagctgtcgt ttcatttccc tgatactgat aactcaggtt gaccccatag ttaaactgat
    23761 cccggcttcc aacggttcct gataatcccg tattatttga ggcaaccccc tgatcatcaa
    23821 acgtcgttga gttagacata tatatttgcc gacgaggcgt cgtaacgtca tcaccccaat
    23881 caaagggaat cgatataaaa atattaaaac gtttctcttc atgatgattc tcgtcataag
    23941 catggcttgc cgcgagggta tagcttatcc ttcgcaggtt gttggaataa ctcaactgat
    24001 aatccttact gctgccgcta cgcccccagt aatctcgcca taacgtactt aaggacacag
    24061 aaccccagcc ttctggcaat gactgactca tattggcaga gaagctattt ttacggccaa
    24121 aatcgttctg gtaataatcg gcaatgtcat agatatcgtt ttcatcacgg cgataattat
    24181 ctttattgtt tgcccatacg tgatcattaa atgtccgata atcacgcgac gaataacgcc
    24241 aggccgccag accaaaacgc gtcgacgttt ggctcacaaa tttgttgtag gcaatttgat
    24301 aactttgccc gtcaaacaca tcgccgttgt cttgtttact atgcgacttc gtggcatcga
    24361 cggaaatggc accaatgcgt gtattccagc cagtccccaa agtgaacgcg taataattat
    24421 tcgcgaccat cgatccgcca tacagcgtca ataagttatt aaaaccatac tgataacccg
    24481 cctgaacaaa atcactttgt ttgctcgccc cttcaatatg gctacgacct gccgcaaaat
    24541 catatttcga cacgccgggt tgcagcatat tcggcaccgc tgcataaggc accagatagg
    24601 tggttaccga accgtccgcc tctttcacgc tgacatcaag atctgctcca ccaccggcca
    24661 actgcaaatc tgtaatcgca aacgggccag gaggaacctc tttctgataa accacaaaac
    24721 cgttctgttc aatagttacc agcgcgttac tctgagcaat cccctgcacc cgtggcgtaa
    24781 aattctgttt cgagttaggc aacatctgca tatcacgaaa caaccgcaca ccgctgaagc
    24841 gaacagaatc aaaaatatcg cttgatgtgt acatatcacc cacgcgaagc gtgccgagaa
    24901 gttgggcaaa tccacgttcc agatacaggg tattgctttt ccacacccct ggattgctat
    24961 ttgttttact gaaactggca tcagaatgca gttgccaccc cagtaagttt aacccgctgt
    25021 taaaacgtac atatgtactc ttgctattac ccgacgcttt atagtcgctg taatactgac
    25081 tcacataata agaggtataa aacgcattaa taccccgctc ccagttttcc ggtggaacat
    25141 agccactttc cagttcttcc acccacgcct gcgggacact gaaatcgaga cgaaaaactc
    25201 cgatatccca ggtatagctc ccaccctgaa caagttgctc aaatgttaaa cattgcttac
    25261 cgctggcaaa gttatcggta ttaatgccta accgcttgat aatttccctt gataaacatg
    25321 tttcttgcgg gttgtcttta acaataatct catatttccc gcgccattgc ttattgacat
    25381 aaatatcgat gtcatactgc cccggcaagg gttgattatc atcaagacga atatttgcaa
    25441 cctgctggtc tttcattcca cctatcataa aatgggtatc aaaggtttct tcagctgcat
    25501 aagcttcaat gccgagcaat aacgctacga ttgctgatgc aagtggggtc attctcaaca
    25561 taggcagctc ctgcaattaa attttgtcac taatatagtt gccatggtca tcgataatgg
    25621 tcagatgcca gttattcgca ttattacttt tgacattaat actctgactt tctaaggggg
    25681 caatcataat agtttcataa ttgactttga cattattagc tttgacatcc gaaatcgtca
    25741 cccaatttgc tgagtcattt tttatcacca atccattgcc actgcgattt acccgtaact
    25801 ttttaaatgt cgctttattt accggtgcaa tacccgcagg ccggtaaaac aatttgattc
    25861 tgttttgcat tgcaaatttc agtgcattct tgccctcttg ctctggacta tttggtggaa
    25921 tatccagaac attcagataa aaaatgctct ctttattagt gggcagttta ttcggcataa
    25981 ttttgatttt tacttgctgc ccggaatttg cccctatttt tgccactggt ggcgttaaca
    26041 tgaaaggaac ctgaattttt tctggtggta acgacgtatc aacatcatca atccacgcct
    26101 gcagcagcga agaacggttt ccctgattca tcaactgcac catcacttct ttattttctg
    26161 ccgggtaaat aatacgcgtc ccgtagataa caataccggc atgtgcagga aggaccatag
    26221 aagaaatgag taaagcaaat aatcctttca tcttaatgcc tcttaaatta aaaggccgcc
    26281 atggggatag cggccatgtt tattacctgg agaaacttga aattttattt attaatcctg
    26341 cgccacacgc ataaccagag aagataacac atcacctgat tgcactgaat tatctgtcag
    26401 cttcaggtat tttgctttaa acggaatagc atattgacct tccgctgttt tataactctc
    26461 aatgttaaaa ggtgcagaaa catccaggac atcgctgtta tcacctaaat agagatagta
    26521 ccccacggta tcgttgctac tggctatcgc cttatcgttt ccggttattt tatcgttggt
    26581 aatcaacgag agttttacac cagcaacttc tttcggacag ctaattttta acatgctgct
    26641 ggttacaccg actgcatcat cataggtcga gtatttagct ttaccttcaa agacagtggt
    26701 tggtacagtt ggcatattaa tacttactga atcgccaccc gcttcgaatt tacacggaga
    26761 ttctgcaata ttccctttaa tggttaatgt tccggtatca acgtctgcgg caaatacccc
    26821 agcgctcata aaaaaagaag aaaagacagc ggcagcaata attgaacgtt tcataatatg
    26881 ttccttaata aaatttattt tttggttgtt aagagtctgt atctcttaga aacaaatttc
    26941 tcacatcaaa aaagttgccg caatgtaaac atattgtttt attgatttaa agcaaattaa
    27001 ctattgcaat ataaatacaa caaatactta agcatattaa atatttatca catctattaa
    27061 ttaatgcaga tttttgtaaa tggtattatt tatatcaata aaaaagctaa aatcattcac
    27121 acgaatgatt tcagctttct aaatccgatt aaaaaaccag ggtggcaaat taatcgtagc
    27181 gatttaacac gattcgacgt ttgctatcgc tacctgcaac aaccacttct gtcgttacac
    27241 ctttgactgg cgaagaaacc tgatgacggg tatcaacatc atacgttaac tgaccacaat
    27301 gctgttgagg ttgtccatta accatagcca tactgcatgt tgggggactg atcctgccat
    27361 taatcgttaa tgtggcagtc tgtgaagaga acgcgggaga agccattcca taggcaagaa
    27421 aaataattcc tgacagccag tacttattca ttgaacgtta tccctgtagt aaaggttatg
    27481 cctggataga atgagtgcat aacaaactat agctgtacat ccactacaca gccacgaagg
    27541 atgataatga agcattgcct gtacgatcaa tcgactttat agagtttcgg acgaaggtcc
    27601 gcagaatatt tgcagcatta aataagtgtt cattaatgcg gcaaaaacaa atacaccgca
    27661 tcaatgtaac atctctgcgg tgtatgaaaa gcaaccaaaa aggtttatac gcgttagacc
    27721 gcgcggaagg aaatctcgcc aggaatgact tctccctgcc aataaagctg ggctgcaacg
    27781 cggtcagcca gctggcggta gatagcggta aattcgctct ccggacggct gataaccgtt
    27841 ggcgtgcctt tatcgagatc ttcacgtaag gagatgtgta acggcatctg ccccagcaac
    27901 tgcgtgtggt atttctcggc cagtttctct gcaccaccgg taccgaaaat cggttcatgg
    27961 tgaccacagt tactgcaaat atgcacactc atgttctcga caataccgag caccggaacc
    28021 tcgactttct cgaacatcac aatgcctttt ttcgcatcga tcaacgcaat gtcctgcggg
    28081 gtggtgacca ctaccgcgcc agttactgga atgttctgtg ccagtgtcaa ttgaatgtca
    28141 ccggtgcctg gcggcatatc aagcaccaga tagtcgagat ccggccacaa ggtttcctgc
    28201 aacatttgca ttaacgcctt gctcgccatc ggaccacgcc acaccatcgc attgtcgtca
    28261 gtgaccagat aaccaattga attggttgca aggccatgag acataatcgg tgccatgtgg
    28321 gtaccgtccg gcgaagttgg acgctgattt tctgcgccca gcatggtggg gattgatggg
    28381 ccgtagatat cggcgtccag aatgccgact ttcgcccctt ccgccgccag tgccagcgcc
    28441 aggtttaccg ccgtggagga tttacccacg ccgcctttac cggagctgac cgcaatgatg
    28501 ttcttcacgc cgttaattcc cggctgattc ttcacgcgtt ttaaggtggc gatattgtgc
    28561 gacagtttcc agtcgatagc tttcgccccg gtgatacgca gcagttcggc gctacattgc
    28621 tcttttaact cttcgaacgc gctatgccag acgaatggca taaccagttc cacatgcaac
    28681 gtgtcgtcca tccaggcgac atggtgcaac gctttcagcg tggtgaggtt atgcttcagg
    28741 gttgggtgct gaaaattggc cagtgtcccg gcgaccattg cgcgcaaggc ttctggtgat
    28801 ttggcctggg attgttcgtt catcccgact ccttttttgt atagataaac catcagctga
    28861 tagtttacct gaagaatata gagaagtact tacttaacat tttcccattt ggtactatct
    28921 aacccctttt cactattaag aagtaatgcc tactatgact caagtcgcga agaaaattct
    28981 ggtgacgtgc gcactgccgt acgctaacgg ctcaatccac ctcggccata tgctggagca
    29041 catccaggct gatgtctggg tccgttacca gcgaatgcgc ggccacgagg tcaacttcat
    29101 ctgcgccgac gatgcccacg gcacaccgat catgctgaaa gctcagcagc tcggtatcac
    29161 cccggagcag atgattggcg aaatgagtca ggagcatcag actgatttcg caggctttaa
    29221 catcagctat gacaactatc actcgacgca cagcgaagag aaccgccagt tgtcagaact
    29281 tatctactct cgcctgaaag aaaacggttt tattaaaaac cgcactattt ctcagctgta
    29341 cgatccggaa aaaggcatgt tcctgccgga ccgttttgtg aaaggcacct gcccgaaatg
    29401 taaatccccg gatcaatacg gcgataactg cgaagtctgc ggcgcgacct acagcccgac
    29461 tgaattgatc gagccgaaat cggtggtttc tggcgctacg ccggtaatgc gtgattctga
    29521 acacttcttc tttgatctgc cctctttcag cgaaatgttg caggcatgga cccgcagcgg
    29581 tgcgttgcag gagcaggtgg caaataaaat gcaggagtgg tttgaatccg gcctgcaaca
    29641 gtgggatatc tcccgcgacg ccccttattt cggttttgaa attccgaacg cgccgggcaa
    29701 atatttctat gtctggctgg acgcaccgat tggctatatg ggctctttca agaatctgtg
    29761 tgataagcgc ggcgacagcg taagcttcga tgaatactgg aagaaagatt ccactgccga
    29821 gctgtatcac ttcatcggta aagatatcgt ttacttccac agcctgttct ggccagcaat
    29881 gctggaaggc agcaacttcc gcaagccgac caacctgttt gttcatggct atgtgacggt
    29941 gaacggcgcg aagatgtcta agtctcgcgg tacctttatt aaggccagca cctggctgaa
    30001 tcattttgac gccgacagcc tgcgttacta ctacactgcg aaactctctt cgcgcattga
    30061 tgatatcgat ctcaacctgg aagatttcgt tcagcgcgtg aatgccgata tcgttaacaa
    30121 agtggttaac ctggcctccc gtaatgcagg ctttatcaac aaacgttttg acggcgtgct
    30181 ggcaagcgaa ctggctgacc cgcagttgta caaaaccttc actgatgccg ctgaagtgat
    30241 tggtgaagcg tgggaaagcc gcgaatttgg taaagcagtg cgcgaaatca tggcgctggc
    30301 tgatctggct aaccgctatg tcgatgaaca ggctccgtgg gtggtggcaa aacaggaagg
    30361 ccgcgatgcc gacctgcagg caatttgctc aatgggcata aacctgttcc gcgtgctgat
    30421 gacttacctg aagccggtac tgccgaaact gaccgagcgc gcagaagcat tcctcaatac
    30481 agaactgacc tgggatggta tccagcaacc gctgctgggc cacaaagtga atccgttcaa
    30541 agcgctgtat aaccgcatcg atatgaagca ggttgaagca ctggtggaag cgtctaaaga
    30601 agaagtgaaa gcagctgccg cgccagttac tggcccactg gcagatgatc cgattcagga
    30661 aaccatcacc tttgacgact tcgccaaagt tgacctgcgc gtggcgctga tcgaaaacgc
    30721 agagtttgtt gaaggttctg acaaactgct gcgcctgacg ctggatctcg gcggtgaaaa
    30781 acgcaatgtc ttctccggca ttcgttctgc ttacccggat ccgcaggcac tgattggtcg
    30841 tcacaccatt atggtggcta acctggcacc acgtaaaatg cgcttcggta tctccgaagg
    30901 catggtgatg gctgccggtc ctggcgggaa agatatcttc ctgctaagcc cggatgccgg
    30961 tgctaaaccg ggtcatcagg tgaaataatc ccccatcaag gcgctgcatc gacagcgcct
    31021 tttctttata aattcctaaa gttgttttct tgcgattttg tctctctcta acccgcataa
    31081 atactgatag catctgcatt caactggata aaattacagg gatgcagaat gagacacttt
    31141 atctttcagg acgaaaaatc acataaattc tgggcggtgg agcagcaggg aaacgaactg
    31201 cacatcaact ggggtaaagt cggcaccaac ggacaaagcc aggtaaaaag ttttgcggat
    31261 gcagcagcgg cggaaaaagc gggactgaag ctgattgcgg agaaggtgaa gaagggatat
    31321 gtggagcaag cgaaggataa ttctttgcaa ccttcccaaa cggtaacgga ctctctcaag
    31381 gtagcgaact tatccaccat tattcaggaa caaccctctt tcgtagcaga aacccgtgcg
    31441 gctgacaaaa atacagatgc tgttttaccg tggctggcga aagatattgc tgtcgttttt
    31501 ccgcccgaag ttgtacacac cacgttaagt catcgccgct ttcccggagt tcctgttcag
    31561 caagcagaca aattgaccca attacgtcgc ttagcctgtg gcgtgtcgca acgggataac
    31621 aaaacagcta catttgactt cagcgcctgt actttagagt ggcaaaactt catcgctcag
    31681 gcgatcgatc tgattaacag cccgaaaacg acaaaattac catcaccggt aatggctgta
    31741 ctcgcggcac ttgaactgca ctgcacgaga tataaagagc gtgaggatgt tatggatcag
    31801 attattcagg aaggcggtct ggaatatgct actgatgtaa taatacatct tcaacaaata
    31861 tctattgaat gggattatgt caacaataat attgttttcc tgtcgtctgg catttcacct
    31921 gattatttgc agcaatattc cagctttgaa ttacgcctac gtaaacattt atcactggcg
    31981 gaagagtctc tctggcaaaa atgtgcacaa aaacttattg ccgcagttcc acatattcca
    32041 gaatggcggc aaccattaat tgctttgtta ttgcccgaaa aaccagaaat tgcacatgaa
    32101 attgcccagc gtctactggg gcaaaagaaa ttaccctcgc tggagtggct aaaaatagtg
    32161 gcgactgatg agcacattct tgcctcatta gaaaaatatc acgagccata tgccattttt
    32221 gatgattact attgtggtgc gatatggtca gccaccgtat tacaggagca aggtgttgcg
    32281 gccctgcccc gatttgctcc ctatgtcgca agtgactact gcgccgatgt gttgcgtcat
    32341 atcaatcatc cgttcgcatt aacactgctt atacgtgtag ccgggcatac taaacgctgt
    32401 cacgatcgaa tgacgaaagc ctgcgctgcg ttcccacacg cagcactggc agcactggcg
    32461 gaacttctag tgcaaaaaga agagaatagt tggcgcatta tgctaatgac gatgcttatc
    32521 tcgcaaccta cactagcaga acaggtcatt ccctggcttt cgacacccgc agttgcagtg
    32581 ctgaaatcat gccagcaaca actgaaacag ccctcaaacc atgccagtgc cgatctactg
    32641 ccggccatag tagtctcccc accatggctt tcgaaaaaga aaaaatcgcc gattccggtg
    32701 cttgatttag caccgctcaa cctcgaatcc atatgcacaa taaccgacac agaagctaaa
    32761 gagtttcaaa ctcattggga ctgggaaccg cataagccag gtgaaggtgc taaagatttc
    32821 ctttactcgc tggggtatcg ccgctgggat tttgacacat ataaatatat cggcgcttct
    32881 gactcagcaa ttgacgcatg ggaacgtgag gacttcgcta cactcatcca aatgtttaaa
    32941 gcccaccatg caccgtatca gggggagtgg catctcaact cacttccctt tttacctatg
    33001 caaaaagcga ttaagctatg ggaatttctc agcaaagagc cgcataccgc gataaaacca
    33061 gtcatgcttt atttgcgact ggcaggtatg agtggttttc tacactcgtt ttcacgctac
    33121 cctcaagaag gttttgcggt tgctaattat tttgccgcaa cggaactggc acctgccgtc
    33181 gcccgcgcct tcaacaaact caaaacccta cgacaagacg cccgcacctg gctgctgaaa
    33241 tacccgcaac acgccattac cggcctgcta cctgcggcac tcggcaaagc cggtgaatcc
    33301 caggataacg cccgtgccgc tttacgtatg ttgattgaaa atggttatcc gtcattactg
    33361 caagaaatcg cccagcgtta taaccagccg gaagtaaccg atgcggtgaa cgctctactt
    33421 gcgctcgatc ccttagataa tcacccgact aaaatcccga cacttccggc cttttatcag
    33481 ccatcgctct ggacgcgccc ggtattaaaa gcaaacgccc aatcactgcc agatagcgcc
    33541 ctcttccacc tcggtgaaat gctccgcttc cctcaggaag atacgctgta tccggggtta
    33601 ttacaggtga aagacgcctg tactgccgac tcactggctg agtttgcctg ggatctgttt
    33661 accgcctggc agaccgctgg cgtgccgtcg aaagagagct gggcgttcac tgcattaggt
    33721 gttcttggca acgatgacac cgcccgcaaa ctgacgccat taatccgcgc ctggcctggc
    33781 gaatctcagc ataaacgtgc caccgttggg ttggatattc tcgctgctat cggcagcgat
    33841 atcgccctga tgcagcttaa cggcatcgcc cagaaactga aattcaaagc cttacaggag
    33901 cgggcgaaag aaaaaattgc cgacattgcc gaaagtcgcg aactcacagt ggcagaactt
    33961 gaagatcggt tagcaccgga tctcggtctg gatgataacg gttcgctgct gctggatttc
    34021 gggccacggc agttcaccgt cagctttgat gaaaccttaa aaccgtttgt gcgtgatgct
    34081 tccggcagcc gcctgaaaga cttgcctaaa ccgaacaaaa gcgatgatga aacccaagcg
    34141 aacgatgcag ttaaccgcta caaactgttg aaaaaagatg cgcgtaccgt tgccgcccag
    34201 caggtagcaa ggctggaatc cgccatgtgc ctgcgccgcc gctggtcgcc ggaaaacttt
    34261 cagctcttcc tggttgagca tccgctggtt cgccacttaa cccgccgtct gatttggggc
    34321 gtttatagca ccgaaaacga gctactggct tgctttcgcg tggcggaaga taacagctac
    34381 agcaccgcag acgatgatct tttcaccctg ccggaaggcg atatctctgt tggcattcct
    34441 cacgttctgg aaatatcacc cacggatgct gccgcctttg gtcaactttt tgccgactac
    34501 gaactgctac cgccgttccg ccagctcgac cgtaacagtt acgccctgac agaagccgag
    34561 cgcaacgcca gcgaactgac ccgctgggca ggcagaaaat gcccgagcgg tcgggtaatg
    34621 gggctggcga ataaaggctg gataaagggc accccgcagg atggaggctg gatcggctgg
    34681 atgatcaaac ctctcggtcg ctggtcgtta atcatggaaa tcaatgaagg atttgcagtt
    34741 ggtatgtcgc cagccgaact cagtgccgag caactcttaa gtaagctgtg gctatgggaa
    34801 ggcaaagcgg aaagctatgg ctgggggagc aattcaacac aggaagcgca gttctccgta
    34861 ctcgatgcca tcaccgccag cgagctaatt aacgatattg aagccctttt tgaataagga
    34921 aagagcatgg acaaggaatt accgtggctg gcggataacg cgcaactgga actgaaatat
    34981 aaaaagggta aaacaccact cagccatcgc aattggccgg gtgaaccggt gcctgttatc
    35041 actgaaagca tcatccagac actgggtgat gaattgctac acattgctga gaagaaaaaa
    35101 aacattgtct ggcgttatga taatttttca ctggagtggc agtccgccat cacgcaggcc
    35161 atcaacttga tcggcgaaca caaaccctca atcccggccc ggacaatggc ggcgctagcc
    35221 tgtatcgcgc aaaatgatag ccaacagtta ctcgacgaaa tcgtccaaca agagggactg
    35281 gaatatgcga ctgaagtggt gattgcacgc cagtttattg tacggtgtta tgagagtgat
    35341 cctctggtag taacattgca gtatcagaag gaggactatg gctatggcta tggctatggc
    35401 tatggctatg gctatggtta tcgctcagaa acctataacg aattcaatct ccgactgcgt
    35461 aagcatctct ctctggcaga ggaaagctgc tggcagcgtt gcgccgacaa actcattgcc
    35521 gcactgccag gactacccca agttcgccgt ccttttattg cactcatcct cccggaaaaa
    35581 ccagaaatcg ccaatgagtt ggtaggcctt gaatgcccgt ggactcattt tcattctaag
    35641 gagtggttaa aagttgttgc tactgaccac agggcagtgg gaaaactcga acgctactgg
    35701 agccaggata tatttagcga tcgcgaagcc agctacatgt cgcatgaaaa ccacttcggc
    35761 tacgcggcct gcgccgccct tttgcgcgaa caaggaattg cagccgttcc acgcctcgcg
    35821 atgtatgccc ataaagaaga ttgcggtagc ctgctggtac aaattaacca tccgcaggtc
    35881 atccgcaccc tgctgttagt tgctgataaa aacaaaccca gcctgcaacg tgtggctaaa
    35941 tacagtaaaa acttccccca tgcgacgctt gccgcactgg cagagcttct ggcgttaaaa
    36001 gaaccgcctg cacgccctgg ttatccaatc atcgaagaca aaaagctgcc tgcacagcaa
    36061 aaagctcgag atgaatactg gcgtacgctg ttacaaacgc tgatggcatc gcagccacaa
    36121 ctggcagaag aggtgatgcc gtggttaagt actcaagccc aggcagtgct gaatagttat
    36181 ttatcggcac cgcccaaaac ggttattgat agcaccgata acatccaaat gcctgaaatc
    36241 ctcgtttcac caccgtggcg tagtaagaaa aaaatgacag ttccacgtct tgatttggca
    36301 ccgttcgaat taacgccgca agtttactgg caaccaggcg aacgagagag gcttgccgcc
    36361 actgagtctg cccgttattt cagcacggaa tctcttgcgg aacgcatgga acaaaaaagt
    36421 ggacgagttg tattacagga gctgggtttt ggagatgatg tatggctgtt tttgaattat
    36481 atactccccg gaaaactgga tgctgcacgc aattcactca ttgttcagtg gcattactac
    36541 ccagggcggg ttgaagagat catgaatggc tggagctccc cggaagctca attagcagaa
    36601 caggcgcttc gcaacggtca cgtagaagtg ttaattaaca tatgggaaaa tgacagctac
    36661 tcacgctatc gtcgggaaaa gagtatctgg aacctgtatt tattggcgca gttgccgcgt
    36721 gagatggctt tgactttctg gctacgtatc aatgagaaaa agcatctgtc tgcgggtgag
    36781 gactattttc tcagtatctt cggattggat gctctaccag gtctgctgtt ggctttttca
    36841 catcgtccaa aagaaacatt tccattaatt ttaaatttcg gcgcaacaga actggccctg
    36901 cccgttgccc gcgtctggcg acgtttcgcg gcgcagcgtg atctggctcg ccagtggatt
    36961 ttacattggc cggaacatac agctactgca cttatccctc ttgtcttcac caaatccagc
    37021 gataatagcg aagctgcatt acttgccctg cgtttacttt acgaacaggg acatggcgaa
    37081 ttactgcaaa ccgtggcaaa ccgctggcag catacagatg tatggcctgc cctggagcaa
    37141 ttacttaaac agggtccaat ggacatttac ccggcacgca ttcccaaagc ccctgatttc
    37201 tggcatccga caatgtggtc cagaccgaga cttatcacga ataatcagcc tgttaccgat
    37261 gatgctctgg aaattatcgg cgacatgctg cgctttaccc agggcggacg tttttattgc
    37321 gggctggaac aactgaaaac gttctgccag ccacaaacgc tggcagcttt tgcctgggat
    37381 ctcttcaccg cgtggcaaca agttggcgcc cccgcaaaag acaactgggc atttctggcg
    37441 ttaagtctct tcggtgacga aagcacggct cgggatctga caacacagat cctcgcctgg
    37501 ccacaggaag gcaaatctgc ccgtgccgtc agtggcctga acatacttac tcagatgaat
    37561 aatgatatgg cgctgataca gctgcatcat atatcgcaac gggcgaaatc ccgcccctta
    37621 cgtgataacg cggcggaatt tcttcaggtg gtcgcagaaa atcgtaggct aagccaggaa
    37681 cagttagcgg acagattagt cccaacactg ggtcttgatg atccgcaggc gttgagtttt
    37741 gattttggtc cacggcaatt taccgttcgc tttgatgaaa accttaaccc ggttatcttt
    37801 gatcagcaaa acgttcgcca gaaaagcgtt ccccggttgc gcgccgatga cgatcaactg
    37861 aaagcgcccg aggcactggc ccgactaaaa gggctaaaaa aagatgcgac tcaggtgagc
    37921 aaaaacctgc tcccgcgtct tgaaacggcc ctacgcacca cccgacgctg gtcgctggca
    37981 gattttcata ctctgtttgt taatcatccc tttacccgcc tggttactca gcgattaata
    38041 tggggcgtgt atccggcaaa tgaaccgcgt cgtttactca acgcctttcg tgtggccgca
    38101 gagggggagt tctgcaatgc acaagatgag cccattgacc tgcctgcgga tgctttgatt
    38161 ggcattgccc acccgttaga aatgacagca gaaatgcgca gtgaatttgc acagcttttt
    38221 gccgattacg aaattatgcc gccttttcgc cagttgacga ggcgcacagt gctgctcacg
    38281 cctgacgagt cagccagtaa cagcctgact cgctgggaag gtaaatccgc taccgttggg
    38341 caacttatgg gaatgcgata caaaggctgg gagtcaggct atgaggacgc atttgtctat
    38401 gacctgggcg agtaccggct ggtccttaag ttttcatccg gttttaacca ctacaatgtt
    38461 gatagcaaag cgctaatgag cttccgttct cttcatttat accgtgacaa taaatctgtc
    38521 acttttgccg aacttgatgt gtttgatttg agtgaagcgt taagcgcacc cgacgtcatt
    38581 ttccattaaa ccacagcggg cagtgatgcg actgcccgtt attaacacga cctgccaccg
    38641 ggaaaagcga tgatcgtgca aaaagagctg gtcgctattt atgattatga agtccctgta
    38701 cctgaagatc cgttttcctt cagacttgag atccataaat gctctgaatt atttacaggt
    38761 tccatctacc ggctggagcg attccggcta catccaacat ttcatcaacg tgatcgagag
    38821 gatatcgacc cgctaataaa tgatgcgttg atttatataa gagatgagtt tattgatgag
    38881 cgaaaattac ggggcgaatc accagaaatg gtaatagcca tttttaatcg tgaactacag
    38941 aatatattca accaagaaat agaaaaatag ctttattaaa gagggagacg gtattccccc
    39001 ttagatgcgt ttcaatttgc ccgttacttc aacaaatcaa tcaaccgtaa cagagccact
    39061 aataactcca gtacggcaat gagcacagtt aacttttgtg tcagcgtgtc catccgctct
    39121 ccttattaag gagcgcaaca ccagcacctt acccttacac tgcctgtcga tactggttta
    39181 cgtatgttgg accacgatgt tgcactccgg atcagggcac tacctgcggc accacccgtc
    39241 cggtagccat gacttgaaat gggttcgatc cggcgcaacg tcccaacagt caggattata
    39301 aatctcatta tcttaattgc aacggggtcc accgatggtt ataattcgtt cgttggaaca
    39361 cgatgttgca caggctgcgg cgttgggcct gaaaatagta aataaaagaa cctgcctcac
    39421 cagcaggttt ttttatttac tgtgatctgc tttccagata ttttccgctc aaacaactaa
    39481 tgcgccaaac atttattgcg cgtaaaatat cgtttatttc attaatacat ttcagggatg
    39541 aatatatgtc accgcagaat aatcatctgc agcgtccgcc tgccgctgtg ttatacgccg
    39601 atgaactggc aaaattaaaa caaaatgatc acgcaccttg cccacccggt tggcagttaa
    39661 gtttgcctgc ggcccgtgct tttatccttg gcgatagcgc gcaaaatatt agccgtaaag
    39721 tggtgattag tccctccgct gtcgaacgta tgttagtcac tcttgccacc gggcgcggtt
    39781 tgatgttggt gggggaacct ggcaccgcaa aatctctcct gtctgaatta ctggcaaccg
    39841 ctattagcgg cgatgccggg ttaaccatcc agggcggcgc atctaccacc gaagatcaaa
    39901 tcaagtacgg ctggaactac gccctgctta tcaaccatgg tccgtcaacg gaagcactgg
    39961 tccctgcccc gctttaccag ggaatgcgcg acggcaaaat cgtccgtttt gaagagataa
    40021 cgcgtacacc gctggaagta caagactgtc tgttaggtat gctttctgac agggtgatga
    40081 cggtgccgga actcaccggt gaagccagtc agctctatgc gcgggaaggc tttaatatta
    40141 ttgccactgc caatacacgc gatcgcggcg tcaatgagat gagtgccgcg ctaaagcgtc
    40201 gcttcgattt tgaaaccgtc tttccgatta tggatttcgc ccaggagctg gaactggtcg
    40261 ccagtgcttc ggcgcatttg ctggcccata gcggtattcc gcataaagtg cctgacgcgg
    40321 tactggagtt actggtccgc accttccgcg atctgcgtgc caacggcgaa aagaaaacgt
    40381 caatggatac actaacagcg attatgtcca ccgctgaagc cgttaacgtg gcccatgctg
    40441 tgggtgtccg cgcctggttt ttagcgaatc gcgcggggga gcctgccgat ctggtggact
    40501 gcattgccgg aaccatcgtc aaagataacg aagaagatcg cgcacgtctg cgccgttact
    40561 tcgaacaacg tgtcgctaca cataaagaag ctcactggca ggcttattat caagctcgcc
    40621 accgcctgcc gtgaggaaag atgcatgagc gagccgttaa ttgtcggcat ccggcatcat
    40681 agtccggcct gcgcccggct ggtgaaatcg ttaatcgaaa gccagcggcc acgatacgtg
    40741 ttgattgaag gcccggctga ttttaatgac cgggtagacg aactgttgtt agcccaccag
    40801 cttccggtag ctatttacag ttattgccag tatcaggacg gtgcagcccc cgggcgtggt
    40861 gcctggacgc catttgctga attttcgccg gagtggcagg cgctacaagc cgcacgtcgt
    40921 attcaggcac aaacttactt catcgatttg ccttgctggg cacaaagtga agaagaggac
    40981 gattcgcctg atatgcaaga ggaaagccag gccttgctgc tgcgtgccac ccgcatggat
    41041 aacagcgaca ccctgtggga tcacttgttc gaagatgaaa gccagcaaac tatattaccc
    41101 tctgcactgg cgcactattt tgcacaactg cggggcgacg cctccggcga tgcactcaat
    41161 cgtcagcgcg aagcctttat ggcccgctgg attgcatggg cgatgcagca aaataatggc
    41221 gacgtattag tcgtctgcgg aggctggcac gctccggcac tggcaaaaat gtggcgcgaa
    41281 tgcccgcaag aaattaacac gccagaattg ctcttactgg atgatgccgt tacaggttgc
    41341 tatctcacgc cctacagtga aaagcgcctt gatgtgctgg caggatacct ttcaggaatg
    41401 cctgccccgg tctggcaaaa ctggtgctgg cggtggggct tgcagcaggc aggtgaacaa
    41461 ctgctgaaaa cggttcttac ccgtttgcgc cagcaccact tgcctgcttc aacagcggat
    41521 atggctgccg ctcatctgca tgcgatggca ctggcacagt tgcgcggtca tacactaccg
    41581 ttacgcactg actggctgga tgccatagca ggctcgctga ttaaagaagc cctgaatgcg
    41641 ccgttgccgt ggagctatcg cggcgttatt catcccgata ccgatccgat tctgctaacg
    41701 ttgatagaca cattagcggg tgacggattc ggtaaacttg ccccttctac gccacaaccg
    41761 cctctgccaa aagatgtcac ctgcgaactg gaacgtaccg caatctctct tccggcggag
    41821 cttaccttaa atcgctttaa ccccaatgga ctagcgcaaa gtcaggtgtt acatcggctg
    41881 gcaatactgg agatcccagg gattgtacgc cagcagggaa gcacactgac acttgcaggt
    41941 aacggtgaag aacgctggaa attaacccgc ccgcttagcc aacatgcggc attaattgag
    42001 gccgcctgct ttggtgccac actccaggaa gccgcacgcc ataaattaga agccgatatg
    42061 ctggacgcgg gtggaatcgg cagtatcacc acatgtctta gccaggcggc gttagcgggt
    42121 ctggcgtcct tcagtcaaca attactggag caacttacat tattaatcgc ccaggaaaat
    42181 cagtttgccg aaatgggcca ggcgctggaa gtgctatatg ccttatggcg gctggatgaa
    42241 attagcggta tgcaaggcgc gcagatatta caaacgacgt tatgcgcggc tatcgatcgc
    42301 acgctgtggc tgtgtgaatc taacggcaga ccggatgaaa aggagtttca cgctcacctg
    42361 catagctggc aagcgctttg ccatatcctg cgcgatctac atagcggcgt taatttaccc
    42421 ggcgtttctc tttctgcggc ggtagcctta ctggagcgac gcagtcaggc gattcatgcc
    42481 ccggcgctgg atcgcggcgc ggctcttggc gcactaatgc gtctggaaca tcccaacgcc
    42541 agtgccgaag cggcgctgac gatgctggcg cagttatccc cggcacaatc cggcgaggcg
    42601 ctgcacggtt tgctggcgct ggcccgccat caactggcct gtcagccggc atttatcgcc
    42661 ggtttcagca gtcatttaaa tcaactgagt gatgccgatt ttatcaatgc cctgcccgat
    42721 ttacgcgcgg cgatggcctg gctaccacca cgagaacgcg ggacactggc gcatcaggtg
    42781 cttgagcatt accagctcgc acagcttccc atttcagcgc tgcaaatgcc gttgcattgt
    42841 ccaccgcaag ccattgcaca tcatcaacaa ctcgaacagc aggcactggc atcgctgcaa
    42901 cactggggag ttttccatgt ctgaactgaa cgatcttctg accacccgtg agctacaacg
    42961 ctggcgatta attcttggcg aagcggcaga aacgaccctt tgtgggctgg atgacaatgc
    43021 ccggcagata gaccacgcgc tggagtggct gtacgggcgc gatcctgaac ggctccagcg
    43081 tggtgaacgc tctggtggat taggtggctc aaatctcacc ccccctgagt ggatcaacag
    43141 tattcacacg ctgtttccgc aacaggtgat tgagcgactg gaaagcgatg ccgtactgcg
    43201 ctacgg
//
LOCUS       DAMZFT010000022        42794 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.022, whole genome shotgun sequence.
ACCESSION   DAMZFT010000022 DAMZFT010000000
VERSION     DAMZFT010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 42794)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42794)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42794
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.022"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(<1..1176)
                     /gene="lysC"
                     /locus_tag="PN977_001383"
     CDS             complement(<1..1176)
                     /gene="lysC"
                     /locus_tag="PN977_001383"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-sensitive aspartokinase 3"
                     /protein_id="HDI6041434.1"
                     /translation="MSEIVVSKFGGTSVADFDAMNRSADIVLSDANVRLVVLSASAGI
                     TNLLVALAEGLEPGERFEKLDAIRNIQFAILERLRYPNVIREEIERLLENITVLAEAA
                     ALATSPALTDELVSHGELMSTLLFVEILRERDVQAQWFDVRKVMRTNDRFGRAEPDVA
                     ALAELAALQLLPRLNEGLVITQGFIGSENKGRTTTLGRGGSDYTAALLAEALHASRVD
                     IWTDVPGIYTTDPRVVSAAKRIDEIAFAEAAEMATFGAKVLHPATLLPAVRSDIPVFV
                     GSSKDPRAGGTLVCNKTENPPLFRALALRRNQTLLTLHSLNMLHSRGFLAEVFGILAR
                     HNISVDLITTSEVSVALTLDTTGSTSTGDTLLTQSLLMELSALCRVEVEEGLALVALI
                     "
     gene            1701..3350
                     /gene="pgi"
                     /locus_tag="PN977_001384"
     CDS             1701..3350
                     /gene="pgi"
                     /locus_tag="PN977_001384"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HDI6041435.1"
                     /translation="MKNINPTQTAAWQALQKHFDEMKDVTIADLFAKDGDRFSKFSVT
                     FDDQMLVDYSKNRITEETLAKLQDLAKECDLAGAIKSMFSGEKINRTENRAVLHVALR
                     NRSNTPILVDGKDVMPEVNAVLEKMKTFSEAIISGEWKGYTGKAITDVVNIGIGGSDL
                     GPYMVTEALRPYKNHLNMHFVSNVDGTHIAEVLKKVNPETTLFLVASKTFTTQETMTN
                     AHSARDWFLKAAGDEKHVAKHFAALSTNAKAVGEFGIDTANMFEFWDWVGGRYSLWSA
                     IGLSIVLSIGFDNFVELLSGAHAMDKHFSTTPAEKNLPVLLALIGIWYNNFFGAETEA
                     ILPYDQYMHRFAAYFQQGNMESNGKYVDRNGNVVDYQTGPIIWGEPGTNGQHAFYQLI
                     HQGTKMVPCDFIAPAITHNPLSDHHQKLLSNFFAQTEALAFGKSREVVEQEYRDQGKD
                     PATLDYVVPFKVFEGNRPTNSILLREITPFSLGALIALYEHKIFTQGVILNIFTFDQW
                     GVELGKQLANRILPELKDDKEISSHDSSTNGLINRYKAWRG"
     gene            3704..3946
                     /locus_tag="PN977_001385"
     CDS             3704..3946
                     /locus_tag="PN977_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6041436.1"
                     /translation="MKKVLYGIFAISALAATSAWAAPVQVGEAAGSAATSVSAGSSSA
                     TSVSTVSSAVGVALAATGGGDGSNTGTTTTTTTSTQ"
     gene            4060..4698
                     /locus_tag="PN977_001386"
     CDS             4060..4698
                     /locus_tag="PN977_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbF family lipoprotein"
                     /protein_id="HDI6041437.1"
                     /translation="MKRPALILICLLLQACSATTKELGNSLWDSLFGTPGVQLTDDDI
                     QNMPYASQYMQLNGGPQLFVVLAFAEDGQQKWVTQDQATLVTQHGRLVKTLLGGDNLI
                     EVNNLAADPLIKPAQIVDGATWTRTMGWTEYQQVRYATARSVFKWDGTDTVKVGSDET
                     PVRMLDEEVSTDQARWHNRYWIDSEGQIRQSEQYLGADYFPVKTTLIKAAKQ"
     gene            4695..5432
                     /locus_tag="PN977_001387"
     CDS             4695..5432
                     /locus_tag="PN977_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC family protein"
                     /protein_id="HDI6041438.1"
                     /translation="MIKQTIVALLLSAGASSVFAAGTVKVFSNGSSEAKTLTGAEHLI
                     DLVGQPQLANSWWPGAVISEELATAAALRQQQALLTRLAELAADSSADDAAAINALRQ
                     QIQALKVTGRQKINLDPDIVRVAERGNPPLQGNYTLWVGPPPSTVTLFGLISRPGKQP
                     FTPGRDVASYLSGQNLLSGADRSYAWVVYPDGRTQKAPVAYWNKRHVEPMPGSIIYVG
                     LADSVWSETSDALNADILQTLTQRIPQ"
     gene            5432..7528
                     /locus_tag="PN977_001388"
     CDS             5432..7528
                     /locus_tag="PN977_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HDI6041439.1"
                     /translation="MKKRHLLSLLALGISTACYGETYPAPIGPSQSDFGGVGLLQTPT
                     ARMAREGELSLNYRDNDQYRYYSASVQLFPWLETTLRYTDVRTRQYSSVEAFSGDQTY
                     KDKAFDLKLRLWEESYWLPQVAVGARDIGGTGLFDAEYLVASKAWGPFDFTLGLGWGY
                     LGTSGNVKNPLCSASDKYCYRDNSYKQAGSIDGSQMFHGPASLFGGVEYQTPWQPLRL
                     KLEYEGNNYQQDFAGKLEQKSKFNVGAIYRVTDWADVNLSYERGNTFMFGVTLRTNFN
                     DLRPSYNDNARPQYQPQPQDAILQHSVVANQLTLLKYNAGLADPQIQAKGDTLYVTGE
                     QVKYRDSREGIIRANRIVMNDLPDGIKTIRITENRLNMPQVTTETDVASLKNHLAGEP
                     LGHETKLAQKRVEPVVPKSTEQGWYIDKSRFDFHIDPVLNQSVGGPENFYMYQLGVMG
                     TADLWLTEHLLTTGSLFANLANNYDKFNYTNPPQDSHLPRVRTHVREYVQNDVYVNNL
                     QANYFQHLGNGFYGQVYGGYLETMFGGVGAEVLYRPLDSNWAFGLDANYVKQRDWRSA
                     KDMMKFTDYSVKTGHLTAYWTPSFAQDVLVKASIGQYLAGDKGGTLEIAKRFDSGVVV
                     GGYATITNVSKEEYGEGDFTKGVYVSVPLDLFSSGPTRSRAAIGWTPLTRDGGQQLGR
                     KFQLYDMTSDRSVNFR"
     gene            complement(7575..7853)
                     /locus_tag="PN977_001389"
     CDS             complement(7575..7853)
                     /locus_tag="PN977_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6041440.1"
                     /translation="MKRNLIKVVKMKPYFAALMLSVSVLPAYAGPLGTADKADLPQSN
                     VSSPMMAQSLRQPDLQPISTDRKTECFRLYTPDRKPGVNCVPDGSTGH"
     gene            8068..8478
                     /gene="psiE"
                     /locus_tag="PN977_001390"
     CDS             8068..8478
                     /gene="psiE"
                     /locus_tag="PN977_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-starvation-inducible protein PsiE"
                     /protein_id="HDI6041441.1"
                     /translation="MTSLSRPRVEFISTILQTVLNLGLLCLGLILVVFLGKETVHLAD
                     VLFAPEQTSKYELVEGLVVYFLYFEFIALIVKYFQSGFHFPLRYFVYIGITAIVRLII
                     VDHKSPLDVLIYSAAILLLVITLWLCNSKRLKRE"
     gene            complement(8572..9462)
                     /gene="malG"
                     /locus_tag="PN977_001391"
     CDS             complement(8572..9462)
                     /gene="malG"
                     /locus_tag="PN977_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001252048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalG"
                     /protein_id="HDI6041442.1"
                     /translation="MAMVQPKSQKARLFITHLLLLLFIAAIMFPLLMVVAISLRQGNF
                     ATGSLIPEQISWDHWKLALGFSVEQADGRITPPPFPVLLWLWNSVKVAGISAIGIVAL
                     STTCAYAFARMRFPGKATLLKGMLIFQMFPAVLSLVALYALFDRLGEYIPFIGLNTHG
                     GVIFAYLGGIALHVWTIKGYFETIDSSLEEAAALDGATPWQAFRLVLLPLSVPILAVV
                     FILSFIAAITEVPVASLLLRDVNSYTLAVGMQQYLNPQNYLWGDFAAAAVMSALPITI
                     VFLLAQRWLVNGLTAGGVKG"
     gene            complement(9477..11021)
                     /gene="malF"
                     /locus_tag="PN977_001392"
     CDS             complement(9477..11021)
                     /gene="malF"
                     /locus_tag="PN977_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalF"
                     /protein_id="HDI6041443.1"
                     /translation="MDVIKKKHWWQSDALKWSVLGLLGLLVGYLVVLMYAQGEYLFAI
                     TTLILSSAGLYIFANRKAYAWRYVYPGMAGMGLFVLFPLVCTIAIAFTNYSSTNQLTF
                     ERAQEVLLDRSWQAGKTYNFGLYPAGDEWQLALSDGETGKNYLSDAFKFGGEQKLQLK
                     ETTAQPEGERANLRVITQNRQALSDITAILPDGNKVMMSSLRQFSGTQPLYTLDGDGT
                     LTNNQSGVKYRPNDQIGFYQSITADGNWGDEKLSPGYTVTTGWKNFTRVFTDEGIQKP
                     FLAIFVWTVVFSLITVFLTVAVGMVLACLVQWEALRGKAVYRVLLILPYAVPSFISIL
                     IFKGLFNQSFGEINMMLSALFGVKPAWFSDPTTARTMLIIVNTWLGYPYMMILCMGLL
                     KAIPDDLYEASAMDGAGPFQNFFKITLPLLIKPLTPLMIASFAFNFNNFVLIQLLTNG
                     GPDRLGTTTPAGYTDLLVNYTYRIAFEGGGGQDFGLAAAIATLIFLLVGALAIVNLKA
                     TRMKFD"
     gene            complement(11175..12365)
                     /gene="malE"
                     /locus_tag="PN977_001393"
     CDS             complement(11175..12365)
                     /gene="malE"
                     /locus_tag="PN977_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000695396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter
                     substrate-binding protein MalE"
                     /protein_id="HDI6041444.1"
                     /translation="MKIKTGARILALSALTTMMFSASALAKIEEGKLVIWINGDKGYN
                     GLAEVGKKFEKDTGIKVTVEHPDKLEEKFPQVAATGDGPDIIFWAHDRFGGYAQSGLL
                     AEITPDKAFQDKLYPFTWDAVRYNGKLIAYPIAVEALSLIYNKDLLPNPPKTWEEIPA
                     LDKELKAKGKSALMFNLQEPYFTWPLIAADGGYAFKYENGKYDIKDVGVDNAGAKAGL
                     TFLVDLIKNKHMNADTDYSIAEAAFNKGETAMTINGPWAWSNIDTSKVNYGVTVLPTF
                     KGQPSKPFVGVLSAGINAASPNKELAKEFLENYLLTDEGLEAVNKDKPLGAVALKSYE
                     EELAKDPRIAATMENAQKGEIMPNIPQMSAFWYAVRTAVINAASGRQTVDEALKDAQT
                     RITK"
     gene            12730..13845
                     /gene="malK"
                     /locus_tag="PN977_001394"
     CDS             12730..13845
                     /gene="malK"
                     /locus_tag="PN977_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011915502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter ATP-binding
                     protein MalK"
                     /protein_id="HDI6041445.1"
                     /translation="MASVQLQNVTKAWGEVVVSKDINLDIHEGEFVVFVGPSGCGKST
                     LLRMIAGLETITSGDLFIGEKRMNDTPPAERGVGMVFQSYALYPHLSVAENMSFGLKL
                     AGAKKEVINQRVNQVAEVLQLAHLLDRKPKALSGGQRQRVAIGRTLVAEPSVFLLDEP
                     LSNLDAALRVQMRIEISRLHKRLGRTMIYVTHDQVEAMTLADKIVVLDAGRVAQVGKP
                     LELYHYPADRFVAGFIGSPKMNFLPVKVTATAIDQVQVELPMPNRQQVWLPVESRDVQ
                     VGANMSLGIRPEHLLPSDIADVILEGEVQVVEQLGNETQIHIQIPSIRQNLVYRQNDV
                     VLVEEGATFAIGLPPERCHLFREDGTACRRLHKEPGV"
     gene            13917..15257
                     /gene="lamB"
                     /locus_tag="PN977_001395"
     CDS             13917..15257
                     /gene="lamB"
                     /locus_tag="PN977_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltoporin LamB"
                     /protein_id="HDI6041446.1"
                     /translation="MMITLRKLPLAVAVAAGVMSAQAMAVDFHGYARSGIGWTGSGGE
                     QQCFQTTGAQSKYRLGNECETYAELKLGQEVWKEGDKSFYFDTNVAYSVAQQNDWEAT
                     DPAFREANVQGKNLIEWLPGSTIWAGKRFYQRHDVHMIDFYYWDISGPGAGLENIDVG
                     FGKLSLAATRSSEAGGSSSFASNNIYDYTNETANDVFDVRLAQMEINPGGTLELGVDY
                     GRANLRDNYRLVDGASKDGWLFTAEHTQSVLKGFNKFVVQYATDSMTSQGKGLSQGSG
                     VAFDNEKFAYNINNNGHMLRILDHGAISMGDNWDMMYVGMYQDINWDNDNGTKWWTVG
                     IRPMYKWTPIMSTVMEIGYDNVESQRTGDKNNQYKITLAQQWQAGDSIWSRPAIRVFA
                     TYAKWDEKWGYDYTGNANTNTNFGKAVPADFNGGSFGRGDSDEWTFGAQMEIWW"
     gene            15500..16420
                     /gene="malM"
                     /locus_tag="PN977_001396"
     CDS             15500..16420
                     /gene="malM"
                     /locus_tag="PN977_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose operon protein MalM"
                     /protein_id="HDI6041447.1"
                     /translation="MKMNKSLIALCLSAGLLASAPGISLADVNYVPQNTSDAPAIPSA
                     ALQQLTWTPVDQSKTQTTQLATGGQQLNVPGISGLVAAYSVPANIGELTLTLTSEVNK
                     QTSVFAPNVLILDQNMTPSAFFPSSYFTYQEPGVMSADRLEGVMRLTPALGQQKLYVL
                     VFTTEKDLLQTTQLLDPAKAYAKGVGNSIPDIPDPVARHTTDGLLKLKVKTNSSSSVL
                     VGPLFGSSAPAPVTVGNTAAPAVAAPAPAPVKKSEPMLNDTESYFNTAIKNAVAKGDV
                     DKALKLLDEAERLGSTSARSTFISSVKGKG"
     gene            16648..18228
                     /locus_tag="PN977_001397"
     CDS             16648..18228
                     /locus_tag="PN977_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SopA family protein"
                     /protein_id="HDI6041448.1"
                     /translation="MIGHITNSGGNDSLMKHDVVQGNNKLDLDLLRNFNGVPGLNRDN
                     FIYISDIFLNIKQRNEKNHAINMFREVSISNDIISVKFYRNEEIECACDFLMDKDAQG
                     YTDLSDLDLTSCHFKGDVISKVSFISSNLQHVTFECKEIGDCNFTTATVDNVIFKCRR
                     LHNVIFIKATGEYVDFSQSILDTVDFSRSQLTHSNFRECQIRNSKFNNCYLYASHFTR
                     AEFLSTKEISFIKSNLTAVMFDHVRMSTGNFKDCITERLELTIDYSDIFGNEELDGYI
                     NNIIKMIDTLPDNAMILKSVLAVKLVMQLKILNIVNKNFIENMKKIFSHCPYIKDPII
                     RSYIHPDEDNKFDNFMRQNRFSKMHFDTQQMIDFINRFNMNKWLIDQNNNFFIQLIDQ
                     ALRSTDDTIKANAWHLYKEWIRSDDVSPLFIEIEDNLRTFNTNELTRNDNIFILFSSV
                     DDGPVMVVSSQRLHDMLNPTKDTNWNSTYIYKSRHEMLPVNLTPETLFGSKSQDKHAL
                     FPIFTASWRANRIKNKGI"
     gene            18451..18948
                     /gene="ubiC"
                     /locus_tag="PN977_001398"
     CDS             18451..18948
                     /gene="ubiC"
                     /locus_tag="PN977_001398"
                     /EC_number="4.1.3.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate lyase"
                     /protein_id="HDI6041449.1"
                     /translation="MSHPALTQLRALRYFKEIPALDPQLLDWLLLEDSMTKRFEQQGK
                     TVSVTMIREGFVEQNEIPEELPLLPKESRYWLREILLCADGEPWLAGRTVVPVSTLSG
                     PELALQKLGKTPLGRYLFTSSTLTRDFIEIGRDAGLWGRRSRLRLSGKPLLLTELFLP
                     ASPLY"
     gene            18961..19833
                     /gene="ubiA"
                     /locus_tag="PN977_001399"
     CDS             18961..19833
                     /gene="ubiA"
                     /locus_tag="PN977_001399"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HDI6041450.1"
                     /translation="MEWSLTQNKLLAFHRLMRTDKPIGALLLLWPTLWALWVATPGVP
                     QLWILAVFVAGVWLMRAAGCVVNDYADRKFDGHVKRTANRPLPSGAVTEKEARALFVV
                     LVLISFLLVLTLNTMTILLSIAALALAWVYPFMKRYTHLPQVVLGAAFGWSIPMAFAA
                     VSESVPLSCWLMFLANILWAVAYDTQYAMVDRDDDVKIGIKSTAILFGQYDKLIIGIL
                     QIGVLALMAIIGELNGLGWGYYWSILVAGALFVYQQKLIANREREACFKAFMNNNYVG
                     LVLFLGLAMSYWHF"
     gene            complement(19988..22411)
                     /gene="plsB"
                     /locus_tag="PN977_001400"
     CDS             complement(19988..22411)
                     /gene="plsB"
                     /locus_tag="PN977_001400"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsB"
                     /protein_id="HDI6041451.1"
                     /translation="MSGWPRIYYKLLNLPLSILVKSKSIPADPAPELGLDTSRPIMYV
                     LPYNSKADLLTLRAQCLAHDLPDPLEPLEIDGTLLPRYVFIHGGPRVFTYYTPKEESI
                     KLFHDYLDLHRSNPNLDVQMVPVSVMFGRAPGREKGEVNPPLRMLNGVQKFFAVLWLG
                     RDSFVRFSPSVSLRRMADEHGTDKTIAQKLARVARMHFARQRLAAVGPRLPARQDLFN
                     KLLASRAIAKAVEDEARSKKISHEKAQQNAIVLMEEIAANFSYEMIRLTDRILGFTWN
                     RLYQGINVHNAERVRQLAHDGHELVYVPCHRSHMDYLLLSYVLYHQGLVPPHIAAGIN
                     LNFWPAGPIFRRLGAFFIRRTFKGNKLYSTVFREYLGELFSRGYSVEYFVEGGRSRTG
                     RLLDPKTGTLSMTIQAMLRGGTRPITLIPIYIGYEHVMEVGTYAKELRGATKEKESLP
                     QMLRGLSKLRNLGQGYVNFGEPMPLMTYLNQHVPDWRESIDPIEAVRPAWLTPTVNNI
                     AADLMVRINNAGAANAMNLCCTALLASRQRSLTREQLTEQLNCYLDLMRNVPYSTDST
                     VPSASASELIDHALQMNKFEVEKDTIGDIIILPREQAVLMTYYRNNIAHMLVLPSLMA
                     AIVTQHRHISRDVLMEHVNVLYPMLKAELFLRWDRDELPDVIDALANEMQRQGLITLQ
                     DDELHINPAHSRTLQLLAAGARETLQRYAITFWLLSANPSINRGTLEKESRTVAQRLS
                     VLHGINAPEFFDKAVFSSLVLTLRDEGYISDSGDAEPAETMKVYQLLAELITSDVRLT
                     IESATQGEG"
     gene            22582..22950
                     /gene="dgkA"
                     /locus_tag="PN977_001401"
     CDS             22582..22950
                     /gene="dgkA"
                     /locus_tag="PN977_001401"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HDI6041452.1"
                     /translation="MANNTTGFTRIIKAAGYSWKGLRAAWINEAAFRQEGVAVLLAVV
                     IACWLDVDAITRVLLISSVMLVMIVEILNSAIEAVVDRIGSEYHELSGRAKDMGSAAV
                     LIAIIVAVITWCILLWSHFG"
     gene            23060..23668
                     /gene="lexA"
                     /locus_tag="PN977_001402"
     CDS             23060..23668
                     /gene="lexA"
                     /locus_tag="PN977_001402"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000646075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repressor LexA"
                     /protein_id="HDI6041453.1"
                     /translation="MKALTARQQEVFDLIRDHISQTGMPPTRAEIAQRLGFRSPNAAE
                     EHLKALARKGVIEIVSGASRGIRLLQEEEEGLPLVGRVAAGEPLLAQQHIEGHYQVDP
                     SLFKPNADFLLRVSGMSMKDIGIMDGDLLAVHKTQDVRNGQVVVARIDDEVTVKRLKK
                     QGNKVELLPENSEFKPIVVDLRQQSFTIEGLAVGVIRNGDWL"
     gene            23741..25066
                     /gene="dinF"
                     /locus_tag="PN977_001403"
     CDS             23741..25066
                     /gene="dinF"
                     /locus_tag="PN977_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter DinF"
                     /protein_id="HDI6041454.1"
                     /translation="MAFLTSSDKALWHLALPMIFSNITVPLLGLVDTAVIGHLDSPVY
                     LGGVAVGATATSFLFMLLLFLRMSTTGLTAQAYGAKNPQALARALVQPLLLALGAGAL
                     IALLRTPIIDLALHIVGGSEAVLEQARRFLEIRWLSAPASLANLVLLGWLLGVQYARA
                     PVILLVVGNILNIVLDVWLVMGLHMNVQGAALATVIAEYATLLIGLLMVRKILKLRGI
                     SGEMLKTAWRGNFRHLLALNRDIMLRSLLLQLCFGAITVLGARLGSDIIAVNAVLMTL
                     LTFTAYALDGFAYAVEAHSGQAYGARDGSQLLDVWRAACRQSGIVALLFSVVYLLAGE
                     HIIALLTSLTQIQQLADRYLIWQVILPLVGVWCYLLDGMFIGATRAAEMRNSMAVAAA
                     GFALTLLTLPWLGNHGLWLALTVFLALRGLSLAAIWRRHWRNGTWFAAT"
     gene            25182..25391
                     /locus_tag="PN977_001404"
     CDS             25182..25391
                     /locus_tag="PN977_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HDI6041455.1"
                     /translation="MNKDEAGGNWKQFKGKVKEQWGKLTDDDMTIIEGKRDQLVGKIQ
                     ERYGYQKDQAEKEVDSWEKRHDYRW"
     gene            complement(25433..25948)
                     /gene="zur"
                     /locus_tag="PN977_001405"
     CDS             complement(25433..25948)
                     /gene="zur"
                     /locus_tag="PN977_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc uptake transcriptional repressor Zur"
                     /protein_id="HDI6041456.1"
                     /translation="MEKTTTQELLAQAEKICAQRNVRLTPQRLEVLRLMSLQDGAISA
                     YDLLDLLREAEPQAKPPTVYRALDFLLEQGFVHKVESTNSYVLCHLFDQPTHTSAMFI
                     CDRCGAVKEECAEGVEDIMHTLAAKMGFALRHNVIEAHGLCAACVEVEACRHPEQCQH
                     DHSVQVKKKSR"
     gene            26266..27270
                     /locus_tag="PN977_001406"
     CDS             26266..27270
                     /locus_tag="PN977_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016235460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2713 family protein"
                     /protein_id="HDI6041457.1"
                     /translation="MSGIISSATEFFNNTLHSNQYEIKDASKEKIKDIYNKSVDYATM
                     SNRAAIIAKIDLFIQRITHNLWTASDNNVSLIKRIDALKNWVEECIDQCTNEQLGDRD
                     YISAFVDRAIFHYAMNFVCNPMENSDVTLIDKCTFDVINHNGLPSTIQLFYDKSYCKA
                     PVASLHLKTVSEGFLNLENAYNVKKDTRLKPLASLLLSFSYDSASADIIGAAERVNDD
                     CENYIKSQIKILSRQVMKKNLAELKDLIDKKELDFNVILQEMKNQAGEEKEDIKNITK
                     MQAAGETLIKFIQSFDPDSTGPIIIGKDMDDKNINDNDLINEDIIKFIYVLLGTELEK
                     "
     gene            27632..28669
                     /gene="dusA"
                     /locus_tag="PN977_001407"
     CDS             27632..28669
                     /gene="dusA"
                     /locus_tag="PN977_001407"
                     /EC_number="1.3.1.-"
                     /EC_number="1.3.1.91"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(20/20a) synthase DusA"
                     /protein_id="HDI6041458.1"
                     /translation="MHGNSEMQKINQTSAMPEKTDVHWSGRFSVAPMLDWTDRHCRYF
                     LRLLSRNTLLYTEMVTTGAIIHGKGDYLAYSEEEHPVALQLGGSDPAALAQCAKLAEA
                     RGYDEINLNVGCPSDRVQNGMFGACLMGNAQLVADCVKAMRDVVSIPVTVKTRIGIDD
                     QDSYEFLCDFINTVSGKGECEMFIIHARKAWLSGLSPKENREIPPLDYPRVYQLKRDF
                     PHLTMSINGGIKSLEEAKAHLQHMDGVMVGREAYQNPGILAAVDREIFGSSDTDADPV
                     AVVRAMYPYIERELSQGTYLGHITRHMLGLFQGIPGARQWRRYLSENAHKAGADINVL
                     EHALKLVADKR"
     gene            28803..29045
                     /gene="pspG"
                     /locus_tag="PN977_001408"
     CDS             28803..29045
                     /gene="pspG"
                     /locus_tag="PN977_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response protein PspG"
                     /protein_id="HDI6041459.1"
                     /translation="MLELLFVIGFFVMLMVTGVSLLGIIAALVVATAIMFLGGMLALM
                     IKLLPWLLLAVAVVWVIKAIKAPKVPKYQRYDRWRY"
     gene            complement(29211..30194)
                     /locus_tag="PN977_001409"
     CDS             complement(29211..30194)
                     /locus_tag="PN977_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HDI6041460.1"
                     /translation="MATRIEFHKHGGPEVLQAVEFTPADPAENEIQVENKAIGINFID
                     TYIRSGLYPPPSLPSGLGTEAAGIVSKVGSGVKHIKAGDRVVYAQSALGAYSSVHNIN
                     ADKAAILPAAISFEQAAASFLKGLTVYYLLRKTYEIKPDEQFLFHAAAGGVGLIACQW
                     AKALGAKLIGTVGTAQKAQSALKAGAWQVINYREENLVERLKEITAGKKVRVVYDSVG
                     RDTWERSLDCLQRRGLMVSFGNSSGAVTGVNLGILNQKGSLYVTRPSLQGYITTREEL
                     TEASNELFSLIASGVIKVDVAEQQKYPLKDAQRAHEILESRATQGSSLLIP"
     gene            30277..31692
                     /gene="dnaB"
                     /locus_tag="PN977_001410"
     CDS             30277..31692
                     /gene="dnaB"
                     /locus_tag="PN977_001410"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HDI6041461.1"
                     /translation="MAGNKPFNKQQAEPRERDPQVAGLKVPPHSIEAEQSVLGGLMLD
                     NERWDDVAERVVADDFYTRPHRHIFTEMARLQESGSPIDLITLAESLERQGQLDSVGG
                     FAYLAELSKNTPSAANISAYADIVRERAVVREMISVANEIAEAGFDPQGRTSEDLLDL
                     AESRVFKIAESRANKDEGPKNIADVLDATVARIEQLFQQPHDGVTGVNTGYDDLNKKT
                     AGLQPSDLIIVAARPSMGKTTFAMNLVENAAMLQDKPVLIFSLEMPSEQIMMRSLASL
                     SRVDQTKIRTGQLDDEDWARISGTMGILLEKRNIYIDDSSGLTPTEVRSRARRIAREH
                     GGIGLIMIDYLQLMRVPALSDNRTLEIAEISRSLKALAKELNVPVVALSQLNRSLEQR
                     ADKRPVNSDLRESGSIEQDADLIMFIYRDEVYHENSDLKGIAEIIIGKQRNGPIGTVR
                     LTFNGQWSRFDNYAGPQYDDE"
     gene            31745..32824
                     /gene="alr"
                     /locus_tag="PN977_001411"
     CDS             31745..32824
                     /gene="alr"
                     /locus_tag="PN977_001411"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HDI6041462.1"
                     /translation="MQAATVVINRRALRHNLQRLRELAPASKMVAVVKANAYGHGLLE
                     TARTLPDADAFGVARLEEALRLRAGGITKPVLLLEGFFDARDLPTISAQHFHTAVHNE
                     EQLAALEEASLDEPVTVWMKLDTGMHRLGVRPEQAEAFYHRLTQCKNVRQPVNIVSHF
                     ARADEPKCGATEKQLAIFNTFCEGKPGQRSIAASGGILLWPQSHFDWVRPGIILYGVS
                     PLEDRSTGADFGCQPVMSLTSSLIAVREHKAGEPVGYGGTWVSERDTRLGVVAMGYGD
                     GYPRAAPSGTPVLVNGREVPIVGRVAMDMICVDLGPQAQDKAGDPVILWGEGLPVERI
                     AEMTKVSAYELITRLTSRVAMKYVD"
     gene            33077..34270
                     /gene="tyrB"
                     /locus_tag="PN977_001412"
     CDS             33077..34270
                     /gene="tyrB"
                     /locus_tag="PN977_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transaminase"
                     /protein_id="HDI6041463.1"
                     /translation="MFQKVDAYAGDPILTLMERFKEDPRSDKVNLSIGLYYNEEGIIP
                     QLKAVADAEARLNAQPHGASLYLPMEGLNSYRHAIAPLLFGADHPVLQQQRVATIQTL
                     GGSGALKVGADFLKRYFPESGVWVSDPTWENHVAIFAGAGFEVSTYPWYDEATNGVRF
                     NDLLATLKTLPARSIVLLHPCCHNPTGADLTNNQWDAVIEILKARELIPFLDIAYQGF
                     GAGMEEDAYAIRAIASAGLPALVSNSFSKIFSLYGERVGGLSVLCEDAEAADRVLGQL
                     KATVRRNYSSPPNFGAQVVAAVLNDEALKASWLAEVEEMRTRILAMRQELVKVLSTEM
                     PERNFDYLLNQRGMFSYTGLSAAQVDRLREEFGVYLITSGRMCVAGLNTANVQRVAKA
                     FAAVM"
     gene            complement(34493..34996)
                     /locus_tag="PN977_001413"
     CDS             complement(34493..34996)
                     /locus_tag="PN977_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001027387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6041464.1"
                     /translation="MNISYVNSNKMTSLPVELDALNNKDISYSKDFSYARDFFLYIET
                     QLKIAKDFCRPGEEVSSSIASTVFHAFIDLVNKIRGKKDCMYICTLCCFAEEVKGDYS
                     HYRTFLFDIGNQYKVKLTQSGKKEFSLTLEFNDTIIESQKVTGNKAKHILEDIEIFYR
                     NKPDTYY"
     gene            35397..36110
                     /gene="aphA"
                     /locus_tag="PN977_001414"
     CDS             35397..36110
                     /gene="aphA"
                     /locus_tag="PN977_001414"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid phosphatase AphA"
                     /protein_id="HDI6041465.1"
                     /translation="MRKITQAISAVCLLFALNSSAVALASSPSPLNPGTNVARLAEQA
                     PIHWVSVAQIENSLAGRPPMAVGFDIDDTVLFSSPGFWRGKKTFSPESEDYLKNPVFW
                     EKMNNGWDEFSIPKEVARQLIDMHVRRGDAIFFVTGRSPTKTETVSKTLADNFHIPAT
                     NMNPVIFAGDKPGQNTKSQWLQDKNIRIFYGDSDNDITAARDVGARGIRILRASNSTY
                     KPLPQAGAFGEEVIVNSEY"
     gene            36221..36637
                     /locus_tag="PN977_001415"
     CDS             36221..36637
                     /locus_tag="PN977_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbQ family protein"
                     /protein_id="HDI6041466.1"
                     /translation="MWYQKTLTLSAKSRGFHLVTDEILNQLADMPRVNIGLLHLLLQH
                     TSASLTLNENCDPTVRHDMERFFLRTVPDNGNYEHDYEGADDMPSHIKSSMLGTSLVL
                     PVHKGRIQTGTWQGIWLGEHRIHGGSRRIIATLQGE"
     gene            36641..36997
                     /locus_tag="PN977_001416"
     CDS             36641..36997
                     /locus_tag="PN977_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HDI6041467.1"
                     /translation="MTISELLQYCMAKPGAEQSVHNDWKATQIKVEDVLFAMVKEVEN
                     RPAVSLKTSPELAELLRQQHSDVRPSRHLNKAHWSTVYLDGSLPDSQIYYLVDASYQQ
                     AVNLLPEEKRKLLVQL"
     gene            complement(37032..39854)
                     /gene="uvrA"
                     /locus_tag="PN977_001417"
     CDS             complement(37032..39854)
                     /gene="uvrA"
                     /locus_tag="PN977_001417"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HDI6041468.1"
                     /translation="MDKIEVRGARTHNLKNINLVIPRDKLIVVTGLSGSGKSSLAFDT
                     LYAEGQRRYVESLSAYARQFLSLMEKPDVDHIEGLSPAISIEQKSTSHNPRSTVGTIT
                     EIHDYLRLLFARVGEPRCPDHDVPLAAQTVSQMVDNVLSQPEGKRLMLLAPIIKERKG
                     EHTKTLENLASQGYIRARIDGEVCDLSDPPKLELQKKHTIEVVVDRFKVRDDLTQRLA
                     ESFETALELSGGTAVVADMDDPKAEELLFSANFACPICGYSMRELEPRLFSFNNPAGA
                     CPTCDGLGVQQYFDPDRVIQNPELSLAGGAIRGWDRRNFYYFQMLKSLADHYKFDVEA
                     PWGSLSANVHKVVLYGSGKENIEFKYMNDRGDTSIRRHPFEGVLHNMERRYKETESSA
                     VREELAKFISNRPCASCEGTRLRREARHVYVENTPLPAISDMSIGHAMEFFNNLKLAG
                     QRAKIAEKILKEIGDRLKFLVNVGLNYLTLSRSAETLSGGEAQRIRLASQIGAGLVGV
                     MYVLDEPSIGLHQRDNERLLGTLIHLRDLGNTVIVVEHDEDAIRAADHVIDIGPGAGV
                     HGGEVVAEGPLEAIMAVPESLTGQYMSGKRKIEVPKKRVPANPEKVLKLTGARGNNLK
                     DVTLTLPVGLFTCITVVSGSGKSTLINDTLFPIAQRQLNGATIAEPAPYRDIQGLEHF
                     DKVIDIDQSPIGRTPRSNPATYTGVFTPVRELFAGVPESRARGYTPGRFSFNVRGGRC
                     EACQGDGVIKVEMHFLPDIYVPCDQCKGKRYNRETLEIKYKGKTIHEVLDMTIEEARE
                     FFDAVPALARKLQTLMDVGLTYIRLGQSATTLSGGEAQRVKLARELSKRGTGQTLYIL
                     DEPTTGLHFADIQQLLDVLHKLRDQGNTIVVIEHNLDVIKTADWIVDLGPEGGSGGGE
                     ILVSGTPETVAECEASHTARFLKPML"
     gene            40109..40645
                     /gene="ssb1"
                     /locus_tag="PN977_001418"
     CDS             40109..40645
                     /gene="ssb1"
                     /locus_tag="PN977_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein SSB1"
                     /protein_id="HDI6041469.1"
                     /translation="MASRGVNKVILVGNLGQDPEVRYMPNGGAVANITLATSESWRDK
                     ATGEMKEQTEWHRVVLFGKLAEVASEYLRKGSQVYIEGQLRTRKWTDQSGQDRYTTEV
                     VVNVGGTMQMLGGRQGGGAPAGGNIGGGQPQGGWGQPQQPQGGNQFSGGAQSRPQQSA
                     PAAPSNEPPMDFDDDIPF"
     gene            complement(40744..41025)
                     /locus_tag="PN977_001419"
     CDS             complement(40744..41025)
                     /locus_tag="PN977_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjcB family protein"
                     /protein_id="HDI6041470.1"
                     /translation="MATLTTGVVLLRWQLLSAVMMFLASTLNIRFRRSDYVGLAVISS
                     GLGVVSACWFAMGLLGITMADITAIWHNIESVMIEEMNQTPPQWPMILT"
     gene            41455..>42794
                     /gene="pdeC"
                     /locus_tag="PN977_001420"
     CDS             41455..>42794
                     /gene="pdeC"
                     /locus_tag="PN977_001420"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="c-di-GMP phosphodiesterase PdeC"
                     /protein_id="HDI6041471.1"
                     /translation="MSHRARHQLLALPGIIFLVLFPIILSLWIAFFWAKSEVNNQLRT
                     FAQLALDKSELVIRQADLVSDAAERYQGQVCTPAHQKRMLNIIRGYLYINELIYARDN
                     HFLCSSLIAPVNGYTIAPADYKREPNVSIYYYRDTPFFSGYKMTYMQRGNYVAVINPL
                     FWSEVMSDDPTLQWGVYDTVTKTFFSLSNEASAATFSPLIHLKDLTVQRNGYLYATVY
                     STKRPIAAIVATSYQRLIAHFYNHLIFALPAGILGSLVLLLLWLRIRQNYLSPKRKLQ
                     RALEKHQLCLYYQPIIDIKTEKCIGAEALLRWPGEQGQVMNPAEFIPLAEKEGMIGQV
                     TDYVIDNVFRDLGAYLATHADRYVSINLSASDFHTSRLIARTNQKTEQYAVRPQQIKF
                     EVTEHAFLDVDKMTPIILAFRQAGYEVAIDDFGIGYSNLHNLKSLNVDILKIDKS"
BASE COUNT        10598 a        10504 c        10976 g        10716 t
ORIGIN      
        1 aatcaacgcg accagcgcca gcccctcttc cacttccacc cggcacagtg cggaaagctc
       61 catcagcagg gattgcgtca gcaacgtatc gccagtggag gttgaaccgg tggtatcaag
      121 ggttaatgcc acgctcactt ctgacgtggt gattaagtct accgaaatat tatgccgcgc
      181 gaggatgccg aaaacttccg cgaggaaacc gcgagaatgc agcatattca ggctgtgcaa
      241 agtgagcaga gtctgattgc gacgaagcgc cagcgcgcgg aacagcggcg gattttcagt
      301 tttattgcac accagcgtac cacctgcgcg tgggtctttg ctggagccga caaagaccgg
      361 gatatcgctg cgtactgcgg gtagcaacgt tgccggatgc agtacttttg caccaaaagt
      421 tgccatctct gccgcttcgg caaacgcgat ttcatcaatg cgttttgctg cggaaaccac
      481 gcgtggatcg gtggtgtaga tgcccgggac gtcggtccag atatcaacac gagatgcgtg
      541 taaagcctcc gccagcaagg ctgccgtata atcgctgcct ccacggccaa gcgtcgttgt
      601 ccgaccttta ttttcgctac cgataaatcc ctgggtgatc actaagcctt cattgaggcg
      661 tgggagcagc tgcagcgcgg ccagttccgc cagcgcggct acatctggct ctgcacgacc
      721 aaatcggtcg ttggtacgca tcactttacg tacatcaaac cactgtgcct gaacatcgcg
      781 ttcgcgcagg atttcgacaa acagcagggt cgacatcagc tcgccgtggc tgaccagttc
      841 atctgtcagg gccggagacg ttgccagcgc cgccgcttct gccagaacag taatattctc
      901 cagcagacgt tcaatctctt cacggataac gttcgggtaa cgcagacgtt ccagaatggc
      961 aaactggata ttgcggattg cgtcgagttt ttcgaatcgc tcgccaggtt ccagtccttc
     1021 agctaaagcg accagcagat tagtgatacc agcagaagcc gagaggacaa ctaaacgtac
     1081 gttggcatca gaaagcacaa tatcagcgct gcggttcatg gcgtcaaaat cagctacgct
     1141 ggtaccgcca aatttggaga caacaatttc agacataact acctcgtgtc aggggatcca
     1201 ttttcagcct tggcacaagg gaagagcgga agacgggtgg gcgcagagcg atacttcgct
     1261 actattttca cccagaagtg ctccaccact tgcgaaacgc ccgactgcga acgcttctgg
     1321 tgacaaccca ggggattcag cccctgtagc cgatgatgaa cgtggccagc cgttcaatca
     1381 cctcggcgat gcaccccctc aggtgttatc acaggactgg ctcctccaac accgttactt
     1441 gggcaacgcg cctcttctgg cctgcgctag cgcaggtagt acatttataa ataaagggtg
     1501 agcggggcgg ttgtcaacga tggggtcatg cggatttttc atccactcct ggcggtcagt
     1561 agttcagcta ataaatgctt cactgcgcta agggtttaca ctcaacatta cgctaacggc
     1621 actaaaacca tcacattttt ctgtgactgg cgctacaatc ttccaaagtc acaattctca
     1681 aaatcagaag agtattgcta atgaaaaaca tcaatccaac gcagaccgct gcctggcagg
     1741 cactacagaa acacttcgat gaaatgaaag acgttacgat cgccgatctt tttgctaaag
     1801 acggcgatcg tttttctaag ttctccgtaa ccttcgacga tcagatgctg gtggattact
     1861 ccaaaaaccg catcactgaa gagacgctgg cgaaattaca agatctggcg aaagagtgcg
     1921 atctggcggg cgcgattaag tcgatgttct ctggcgagaa gatcaaccgc actgaaaacc
     1981 gcgccgtact gcacgtagcg ctgcgtaacc gtagcaatac cccgattctg gttgatggca
     2041 aagatgttat gccggaagtc aacgcggtgc tggagaagat gaaaaccttc tcggaagcga
     2101 ttatttccgg tgagtggaaa ggctataccg gcaaagcaat cactgacgta gtgaacatcg
     2161 ggatcggtgg ttctgacctc ggcccgtaca tggtgaccga agcactgcgt ccgtacaaaa
     2221 accacctgaa catgcacttt gtttctaacg tcgatgggac tcacatcgcg gaagtgctga
     2281 aaaaagtaaa cccggaaacc acgctgttcc tggtagcatc taaaaccttc accactcagg
     2341 aaaccatgac caacgcccat agcgcgcgtg actggttcct gaaagcggca ggtgatgaga
     2401 aacacgttgc gaaacacttt gcggcgcttt ccaccaatgc caaagccgtt ggcgagtttg
     2461 gtattgatac tgccaacatg ttcgaattct gggactgggt cggcggtcgt tactctttgt
     2521 ggtcagcgat tggcctgtcg attgttctct ccatcggctt tgataacttc gttgaactgc
     2581 tttctggcgc acacgcgatg gacaagcatt tctccaccac gcctgccgag aaaaacctgc
     2641 ctgtcttgct ggcgctgatt ggcatctggt acaacaattt ctttggtgcg gaaactgaag
     2701 cgattctgcc gtatgaccag tatatgcacc gtttcgcggc gtacttccag cagggcaata
     2761 tggagtccaa cggtaagtat gttgaccgta acggtaacgt tgtggattac cagactggcc
     2821 cgattatctg gggtgaacca ggtactaacg gtcagcacgc gttctaccag ctgatccacc
     2881 agggaaccaa aatggtaccg tgcgatttca tcgctccggc tatcacccat aacccgctct
     2941 ctgatcatca ccagaaactg ctgtctaact tcttcgctca gaccgaagcg ctggcgtttg
     3001 gtaaatcccg cgaagtggtt gagcaggaat atcgcgatca gggtaaagat ccggcaacgc
     3061 ttgactacgt ggtgccgttc aaagtgttcg aaggtaaccg cccgaccaac tccatcctgc
     3121 tgcgtgaaat cactccgttc agcctgggtg cgttgattgc gctgtatgag cacaaaatct
     3181 ttactcaggg cgtgatcctg aacatcttca ccttcgacca gtggggcgtg gagctgggta
     3241 aacaactggc gaaccgtatt ctgccagagc tgaaagatga caaagaaatc agcagccacg
     3301 atagctcgac caatggtctg attaaccgct ataaagcgtg gcgcggttaa ttaaataacc
     3361 ctaataaaaa tgccgacgtt atgtcggcat tttttatcag ataaatcccc ttgtctgtga
     3421 tttaacggaa atcataccgt gaggttaatc ctaaaataga tttttaatcg tgattgattt
     3481 cggaaaatac gcagattaat tacttttatt tttattttaa gcttatgatt tttagtgtta
     3541 tttatatata atctgaagct tatatttgat attcattcca attatcctaa aaccccatca
     3601 ctaattaccc gcgccgtaat tcgcatgctt tagttgtgta tactcgatcc cgcccgaaat
     3661 gtttttgggt aaatctccat tcattcaatg aagggaaatt gttatgaaaa aagttctgta
     3721 tggcattttt gccatatctg cgcttgcggc gacttctgcg tgggctgcac ctgtacaggt
     3781 gggcgaagcg gcagggtcgg cagcaacgtc ggtttcggcg gggagttcct ccgcgaccag
     3841 cgtcagcacc gtaagctcgg cggtgggtgt cgcgctcgcg gcaaccggtg gcggtgatgg
     3901 ttctaatacc gggaccacca caaccacgac caccagtacc cagtaataaa gtatgtatcc
     3961 ccaaaataat tcgagtcatc gcatctgtgg cttgaagtat gaagggattt accataacca
     4021 cactccggtg tggttattct gcccctctgg agaagagtcg tgaagcgacc tgcactcatt
     4081 cttatctgcc tgctattaca ggcctgttca gccacgacta aagagctggg caattcactg
     4141 tgggacagtc tgttcggtac gccaggcgta caactgacgg acgatgatat tcaaaatatg
     4201 ccctacgcca gccagtacat gcagcttaat ggcgggccgc agttatttgt ggtgctggcc
     4261 ttcgctgaag acggacaaca aaaatgggtc actcaggatc aggccaccct tgtcacacaa
     4321 catggtcgtc tggtgaagac tttgcttggc ggcgacaacc tgattgaagt gaataacctt
     4381 gctgccgacc cgctgattaa acccgcacaa attgttgatg gtgcaacctg gacgcgcacg
     4441 atgggctgga ccgagtacca gcaggtacgc tacgccaccg cacgctcagt cttcaaatgg
     4501 gatggcaccg ataccgtcaa ggtcggcagc gatgaaaccc cggttcgcat gctggacgaa
     4561 gaagtctcca ccgaccaggc gcgctggcat aaccgctact ggatcgacag cgaagggcaa
     4621 attcgccagt cggaacagta tctcggcgcg gattatttcc cggtgaaaac caccctcatc
     4681 aaggcggcaa aacaatgatt aaacaaacta ttgtcgcgtt gcttttgagt gcgggggcgt
     4741 catcagtctt cgcggcggga accgtcaagg tgttcagcaa tggcagcagt gaggctaaaa
     4801 cgctgacggg cgcagagcat ttaatcgatc tggtaggcca accgcagctg gcaaacagct
     4861 ggtggcctgg tgcggtgatt agcgaagagc tggccacggc ggcagcattg cgtcagcagc
     4921 aggcgttgct gacacggctg gcagaactgg ctgcagattc cagcgccgac gatgccgccg
     4981 caattaacgc cttacgccag caaattcagg cgttgaaagt gacgggcagg caaaaaatca
     5041 atcttgatcc tgatatcgtc cgcgttgccg aacgcggtaa cccgccgttg cagggcaact
     5101 acacgctgtg ggtcggacca ccgccgtcca cggtcacgtt attcgggctt ataagccgtc
     5161 ctggcaagca gccattcact ccaggtcgcg acgtggcgag ctatctctcc gggcagaacc
     5221 tgcttagcgg tgcggatcgc agctacgcgt gggtggttta cccggacgga cgcacgcaaa
     5281 aagcgccggt ggcttactgg aacaagcgtc acgtagagcc gatgcccggc agcattattt
     5341 atgttggcct cgcggactcc gtctggagtg agacgtctga tgcccttaac gccgacattc
     5401 ttcagactct gacgcagcgg atacctcaat aatgaaaaaa agacatctgc ttagcttact
     5461 ggcgctgggc attagcacag cttgctacgg cgaaacatat cctgcgccca ttggtccgtc
     5521 gcagtcggat ttcggtggcg taggattatt acaaacgccc accgcgcgca tggcgcggga
     5581 aggggagttg agcctgaact atcgcgataa cgatcagtac cgttattact cagcttcagt
     5641 gcaactcttc ccgtggctgg aaacaacgct gcgctacacc gacgtgcgca cccggcagta
     5701 cagcagcgtc gaagcgttct ctggtgatca aacgtataaa gataaagcct tcgatctcaa
     5761 actgcgtttg tgggaagaga gttattggct gccgcaagtg gcggttggtg cgcgggatat
     5821 cggcggtacg gggctgtttg atgcggaata tcttgtcgcc agcaaagcct gggggccgtt
     5881 cgattttacg ctcggcctgg gctggggata tctgggcacc agcggtaatg tgaaaaatcc
     5941 gctctgttca gccagtgata aatattgcta tcgcgataac agctataaac aggcgggatc
     6001 tatcgacggt agccagatgt tccacggccc ggcctcactg tttggcggcg tggaatacca
     6061 gacgccctgg caaccgctgc gcctgaaact ggagtatgaa ggcaataatt atcagcagga
     6121 ttttgccggg aagctggagc aaaaaagtaa gtttaacgtc ggggcgattt atcgcgttac
     6181 cgattgggcc gacgttaacc ttagctatga acgtggcaac acctttatgt ttggcgtcac
     6241 gctgcgcacc aactttaacg atctgcgtcc ttcgtataac gataacgccc gcccgcaata
     6301 tcaaccgcag ccacaggatg ccattttgca gcattcggtg gtggcgaatc agttaacgct
     6361 gttgaaatac aacgccggac tggccgatcc gcagatccag gcgaaaggcg atacgctgta
     6421 tgttaccggc gagcaggtga aatatcgtga ttcgcgcgaa gggatcatcc gtgctaatcg
     6481 gatcgtgatg aacgatctgc cggatgggat caaaacgatc cgcattacgg aaaatcgcct
     6541 taacatgccg caggtgacga cggaaaccga tgtcgccagc ctgaaaaatc atctcgccgg
     6601 agagccgttg ggccacgaaa cgaagctggc gcaaaaacgc gtcgagccag tggttccgaa
     6661 gtccaccgag cagggctggt atattgacaa atcacgcttt gatttccata tcgatccggt
     6721 gctgaaccag tcggtcggtg gcccggaaaa cttttacatg tatcagctgg gcgtgatggg
     6781 aacggcggat ttgtggctga cggagcatct gctcaccacc ggcagcctgt ttgctaacct
     6841 tgccaacaac tacgacaagt ttaactacac caatccaccg caggactcgc acttaccgcg
     6901 cgttcgtacc catgtgcgcg agtatgtgca gaatgatgtc tatgtgaata acctgcaagc
     6961 caactacttc cagcatctgg gcaacggctt ctacggtcag gtctacggag gttatctcga
     7021 aaccatgttt ggcggcgtgg gggctgaagt gttgtatcgc ccgctggaca gcaactgggc
     7081 gtttggtctg gatgccaact acgttaaaca gcgcgactgg cgtagcgcaa aagatatgat
     7141 gaaattcacc gattacagcg tgaaaaccgg acatctgacc gcctactgga cgccatcttt
     7201 cgctcaggat gtgttagtga aagccagcat cgggcagtat ctggcagggg ataaaggcgg
     7261 cacgctggag atcgccaaac gctttgatag cggcgtggtg gtgggtggct atgccacgat
     7321 caccaatgtt tcgaaagagg agtacggcga aggggacttc accaaaggcg tatatgtctc
     7381 tgtaccgctg gatctcttct cgtctggccc aacacgcagt cgtgcggcaa ttggctggac
     7441 gccgctgacg cgtgacggtg gtcagcaact tgggcgtaag ttccagttgt atgacatgac
     7501 cagcgaccgt agcgtcaatt tccgctaagt catgggaaag gtgccagttt tcgcactcac
     7561 tggcaccttt attcttaatg gcctgtcgag ccatcaggaa cgcagttcac ccccggtttg
     7621 cgatctggtg tgtaaaggcg aaaacattct gtcttcctgt ccgtcgatat tggttgcagg
     7681 tctggttgcc tgagcgactg ggccatcatt gggctggaga cattgctttg cggtaggtcg
     7741 gctttatcag cagtaccgag cggaccggca taagcaggaa gaacagagac tgataacatc
     7801 aaagcagcaa aataaggctt catttttacc acctttatca ggttacgttt catttgttcc
     7861 agaggaacat tgtcgatttt tcgcgcattg ctggtggctg ggaatcacct gaatgggtga
     7921 tttttttggg ctgtgtgttt tgccagatgc gacgttgatg ggagatgccc acttaattga
     7981 gctgaacaaa acatacacaa aaaatataga tctccgtcac atttttgcgt tatacaggaa
     8041 gctcgccact gtgaaggagg tactgctatg acgtcactct ctcgtccgcg cgtggagttt
     8101 atctccacca ttttgcagac cgtactcaat cttggcctgc tgtgcctcgg ccttattttg
     8161 gttgtcttcc tcggcaaaga aacggtgcat ctggctgatg tgctgttcgc gccagaacaa
     8221 accagcaaat atgagctggt agaagggctg gtggtttact ttctctattt cgaatttatc
     8281 gcgctgattg tgaagtactt tcagtccggt tttcacttcc cgctacgtta ctttgtctat
     8341 attgggatca ccgccattgt gcgcttgatc atcgtcgatc ataaatcgcc actggatgtg
     8401 ttgatctact cggcagcgat cctgttactg gtgatcaccc tgtggctgtg taattcgaaa
     8461 cggctgaagc gggagtaaaa aaaggcgctc cgtggagcgc cgaataacag tcacaagttg
     8521 ggataacgta agttgagggt gcagcggcat aacattggca gaacaacatc tttaaccttt
     8581 cacaccacct gccgtcaggc cgttgaccag ccagcgttga gccagcagga agacgatggt
     8641 gatcggtaat gcagacatca cggcagcggc ggcaaagtca ccccacaggt agttttgcgg
     8701 gttgaggtat tgctgcatcc ccacagccag ggtgtagctg tttacgtcac gcagtaatag
     8761 cgacgcgacc ggaacttcag taatggcagc gataaacgac aggatgaaca ccaccgccag
     8821 aatcggtact gacagcggca acaggacaag gcggaaggcc tgccacggtg tcgcaccatc
     8881 cagcgccgcc gcttcttcca gcgaactgtc gatggtttcg aaataacctt tgatggtcca
     8941 gacatgcagc gcaatcccgc ccagatacgc gaaaattacg ccgccgtgag tattcagacc
     9001 aatgaatgga atgtactcac ccagacgatc aaacaacgca tacaacgcga ccagtgaaag
     9061 tactgccggg aacatctgga aaatcagcat tcctttcagc agcgtcgctt tgcctggaaa
     9121 gcgcatacgg gcgaaagcgt aggcgcaggt ggtggagagc gccacaatgc caatcgcgga
     9181 aatcccggcg acctttaccg agttccacag ccacagcagt accgggaatg gcggtggcgt
     9241 aatgcgacca tcagcctgtt ccacactaaa acctaacgcc agtttccagt gatcccagga
     9301 gatttgctcc gggatcaggc tgccagtcgc gaagttcccc tgacgcagcg agatggcgac
     9361 gaccatcagc agcgggaaca taatcgccgc gataaaaagt agcagtagca ggtgagtaat
     9421 aaacaaacgt gctttttgcg atttcggttg gaccattgcc atttttgtta tctcccttaa
     9481 tcaaacttca ttcgcgtggc tttcaggttc actatcgcca gcgcacccac cagcaggaag
     9541 atcagcgtgg caattgctgc tgccagaccg aagtcctgac ccccgccgcc ttcaaaagcg
     9601 atgcggtagg tgtagttaac aagcaggtcg gtataaccgg ctggcgtggt cgtgccaaga
     9661 cgatccgggc cgccgttggt taacagttga atcagcacga agttgttaaa gttaaaggcg
     9721 aagctggcga tcatcagcgg cgtcagcggt ttaatcagca gcggcagcgt aatcttaaag
     9781 aagttctgga acggacctgc gccatccatt gctgaggctt catacaaatc gtccggaatc
     9841 gctttcagca agcccatgca gaggatcatc atgtacggat aacccagcca ggtattgacg
     9901 ataatcagca tcgttcgggc ggtggtcgga tcgctgaacc aggcgggctt cacgccaaac
     9961 agcgcgctca acatcatgtt gatctcaccg aagctctggt taaacaaccc tttgaaaatc
    10021 aagattgaaa tgaacgatgg caccgcgtag ggcagaataa gcaggacgcg atagaccgct
    10081 ttgccgcgca acgcttccca ctgcaccaga cacgccagaa ccatgccgac cgccaccgtt
    10141 aaaaagacag tgatcagcga gaacaccacg gtccagacga aaatggcgag gaacggtttc
    10201 tgaatgcctt cgtcggtaaa gacgcgggta aagtttttcc agccggtggt cacggtgtaa
    10261 ccggggctta gcttttcatc accccagttg ccgtcagcag tgatggactg gtaaaaacca
    10321 atctggtcgt ttggacgata ttttacgccg ctctgattat tcgtcagcgt gccgtcaccg
    10381 tcgagtgtgt agagcggctg cgtgccagaa aactggcgca gggagctcat catcactttg
    10441 ttgccatccg gcagaatggc ggtaatgtca ctcagtgcct gacgattctg ggtaatcacg
    10501 cgcagattcg cgcgttcgcc ttcgggctgg gcggtcgttt ctttcagttg cagtttttgc
    10561 tcgccgccaa atttaaacgc gtcggagagg taatttttgc cggtttcgcc gtcgctgagc
    10621 gccagttgcc actcatcgcc cgccgggtaa agaccaaaat tataggtttt gcctgcttgc
    10681 caggagcgat ctaacaacac ttcctgcgca cgttcaaaag tcagctggtt agtgctgctg
    10741 tagttggtaa aggcaatggc gatggtgcag accaaaggga agaggacgaa taatcccatt
    10801 ccagccattc ccgggtaaac atagcgccag gcgtaggctt tacgattggc gaaaatatac
    10861 agccccgctg aactcaatat cagcgtggta atggcgaaca ggtattcccc ttgtgcgtac
    10921 attaaaacaa caaggtaacc caccagcagg ccgagcagac ctaacactga ccatttcagc
    10981 gcgtcgcttt gccaccaatg tttcttttta atgacatcca tggggttctt cctcattcca
    11041 ggacggataa ggctttcacg ccttatccga caacaactgc ctgatgcgac gctgacgcgc
    11101 cttaccaggc ctacatacgt ttcggttttg taggccggac aaggcgttca cgccgcatcc
    11161 ggcatttcac agcattactt ggtgatacga gtctgcgcgt ctttcagggc ttcatcgaca
    11221 gtctgacgac cgctggcggc gttgatcacc gcagtacgca cggcatacca gaaagcggac
    11281 atctgcggga tgttcggcat gatttcacct ttctgggcgt tttccatggt ggcggcaata
    11341 cgtggatctt tcgccaactc ttcctcgtaa gacttcagcg ccactgcacc cagcggttta
    11401 tctttattga ccgcttccag accttcgtca gtcagcagat agttttcgag gaactctttc
    11461 gccagctctt tgttcggact ggcggcgtta ataccggcgc tcagcacgcc aacgaacggt
    11521 ttggatggtt gacccttgaa ggtcggcagt accgttacac cataattcac tttgctggtg
    11581 tcgatgttgg accatgccca cgggccgttg atggtcatcg ctgtttcgcc tttattaaag
    11641 gcagcttctg cgatggagta atcggtgtct gcattcatgt gtttgttttt aatcaggtca
    11701 accaggaagg tcagacccgc tttcgcgcca gcgttatcca cgcccacgtc tttaatgtcg
    11761 tacttgccgt tttcatactt gaacgcataa cccccgtcag ccgcaatcag cggccaggtg
    11821 aagtatggtt cttgcaggtt gaacatcagc gcgctcttac ctttcgcttt cagttcttta
    11881 tccagcgccg ggatctcttc ccaggttttt ggcgggttcg gcagcagatc tttgttataa
    11941 atcagcgata acgcttcgac agcaatcggg taagcaatca gcttgccgtt gtaacgaacg
    12001 gcatcccagg taaacggata cagcttgtcc tggaacgctt tgtccggggt gatttcagcc
    12061 aacaggccag attgagcgta gccaccaaag cggtcgtgcg cccagaagat aatgtcaggg
    12121 ccatcgccag ttgccgcaac ctgagggaat ttctcttcca gtttatccgg atgctcgacg
    12181 gtgactttaa ttccggtatc tttctcgaat ttcttaccga cttcagcgag accgttatag
    12241 cctttatcgc cgttaatcca gattaccagt ttaccttctt cgattttggc gagagccgag
    12301 gcggaaaaca tcatcgtcgt taatgcggat aatgcgagga tgcgtgcacc tgtttttatt
    12361 ttcataatct atggtccttg ttggtgaagt gctcgtgaaa acacctaaac ggactctagt
    12421 ttctttatac ggcaacctct ttccatcctc cttgccccta cgccccaccg ttgctttgtg
    12481 tgatctctgt tacagaattg gcggtaatgt ggagatgcgc acataaaatc gccacgattt
    12541 ttgcaagcaa catcacgaaa ttccttacat gacctcggtt tagttcacag aagccgcgtt
    12601 ctcatcctcc cgcctcctcc cccataaaaa agccaggggg tggaggattt aagccatctc
    12661 ctgatgacgc atagtcagcc catcatgaat gttgctgtcg atgacaggtt gttacaaagg
    12721 gagaagggca tggcgagcgt acagctgcaa aatgtaacga aagcctgggg cgaggtcgtg
    12781 gtatcgaaag atatcaatct cgatatccat gaaggtgaat tcgtggtgtt tgtcggaccg
    12841 tctggctgcg gtaagtcgac tttactgcgc atgattgccg ggcttgagac gatcaccagc
    12901 ggcgacctgt tcatcggtga gaaacggatg aatgacactc cgccagcaga acgcggcgtt
    12961 ggtatggtgt ttcagtctta cgcgctctat ccccacctgt cagtagcaga aaacatgtca
    13021 tttggcctga aactggcagg cgcaaaaaaa gaggtgatta accaacgcgt caaccaggta
    13081 gcggaagtgc tgcaactggc gcatttgctg gatcgcaaac cgaaagcgct ctccggtggt
    13141 cagcgtcagc gtgtggcgat tggccgtacg ctggtggccg agccaagcgt atttttgctc
    13201 gatgaaccgc tctccaacct cgatgctgca ctgcgtgtgc aaatgcgtat cgaaatctcc
    13261 cgtctgcata aacgcctggg ccgcacaatg atttacgtca cccacgatca ggtcgaagcg
    13321 atgacgctgg ccgacaaaat cgtggtgctg gacgctggtc gcgtggcgca ggttgggaaa
    13381 ccgctggagc tgtaccacta tccggcagac cgttttgtcg ccggatttat cggttcgcca
    13441 aagatgaact tcctgccagt aaaagtgacc gccactgcaa tcgatcaagt gcaggtggag
    13501 ctgccgatgc caaatcgtca gcaagtctgg ctgccggttg aaagccgtga tgtccaggtt
    13561 ggagccaata tgtcgctggg tattcgcccg gaacatctac tgccgagtga tatcgctgac
    13621 gtcatccttg agggtgaagt tcaggtcgtc gagcaactcg gcaacgaaac ccaaatccat
    13681 atccagatcc cttccattcg tcaaaacctg gtgtaccgcc agaacgacgt ggtgttggta
    13741 gaagaaggtg ccacattcgc tatcggcctg ccgccagagc gttgccatct gttccgtgag
    13801 gatggcactg catgtcgtcg actgcataag gagccgggcg tttaagcacc ccacaaaaca
    13861 cacaaagcct gtcacaggtg atgtgaaaaa agaaaagcaa tgactcagga gatagaatga
    13921 tgattactct gcgcaaactt cctctggcgg ttgccgtcgc agcgggcgta atgtctgctc
    13981 aggcaatggc tgttgatttc cacggctatg cacgttccgg tattggctgg acaggtagcg
    14041 gcggtgaaca acagtgtttc cagactaccg gtgctcaaag taaataccgt cttggcaacg
    14101 aatgtgaaac ttatgctgaa ttaaaattgg gtcaggaagt gtggaaagag ggcgataaga
    14161 gcttctattt cgacactaac gtggcctatt ccgtcgcaca acagaatgac tgggaagcta
    14221 ccgatccggc cttccgtgaa gcaaacgtgc agggtaaaaa cctgatcgaa tggctgccag
    14281 gctccaccat ctgggcaggt aagcgcttct accaacgtca tgacgttcat atgatcgact
    14341 tctactactg ggatatttct ggtcctggtg ccggtctgga aaacatcgat gttggcttcg
    14401 gtaaactctc tctggcagca acccgctcct ctgaagctgg tggttcttcc tctttcgcca
    14461 gcaacaatat ttatgactat accaacgaaa ccgcgaacga cgttttcgac gtgcgtttag
    14521 cgcagatgga aatcaacccg ggcggcacat tagaactggg tgtcgactac ggtcgtgcca
    14581 acctgcgtga taactatcgt ctggttgatg gcgcatcgaa agacggttgg ttattcactg
    14641 ctgaacatac tcagagtgtc ctgaagggct ttaacaagtt tgttgttcag tacgctactg
    14701 actcgatgac ctcacagggt aaaggtctgt cgcagggttc tggcgtcgcg tttgataacg
    14761 aaaaatttgc ctacaatatc aacaacaacg gtcacatgct gcgtatcctc gaccacggtg
    14821 cgatctccat gggcgataac tgggacatga tgtacgtggg tatgtaccag gatatcaact
    14881 gggataacga caacggcacc aagtggtgga ccgtcggtat tcgcccgatg tacaagtgga
    14941 cgccaatcat gagcaccgtg atggaaatcg gctacgacaa cgtcgaatcc cagcgcaccg
    15001 gcgacaagaa caatcagtac aaaattaccc ttgcacaaca atggcaggct ggcgacagca
    15061 tctggtcacg cccggctatt cgtgtcttcg caacctacgc caagtgggat gagaaatggg
    15121 gttacgacta caccggtaac gccaatacca acactaactt cggcaaagcc gttcctgctg
    15181 atttcaacgg cggcagcttc ggtcgtggcg acagcgacga gtggaccttc ggtgcccaga
    15241 tggaaatctg gtggtaatag caaaacctgg gccggataag gtgcttgcgc cgcatccggc
    15301 aaccaacgcc tgatgcgacg cttgcgcgtc ttatcaggcc tacaacggct gtcaaatgta
    15361 ggccggataa ggcgtttgcg ccgcatccgg cataaaaaca ggttgtcatt atctgaaagg
    15421 ggcgaaagcc cctctgatta tcgggtttag cgcgctattg cctggctacc gctgagctcc
    15481 agattttgag gtgaaaacaa tgaaaatgaa taaaagtctc atcgccctct gtttatcagc
    15541 agggttactg gcaagcgcgc ctggaatcag ccttgccgat gttaactacg taccgcaaaa
    15601 caccagcgac gcgccagcca ttccatctgc cgcgctgcaa caactcacct ggacaccggt
    15661 cgatcaatct aaaacccaga ccacccaact ggcgaccggc ggccaacaac tgaacgtccc
    15721 cggcatcagt ggtctggttg ctgcgtacag cgtcccggca aacattggcg aactgacact
    15781 gacgctgacc agcgaagtga acaaacaaac cagcgtattt gcgccgaacg tgctgattct
    15841 tgatcagaac atgaccccat cagccttctt ccccagcagt tatttcacct accaggaacc
    15901 aggcgtgatg agtgcagatc ggctggaagg cgttatgcgc ctgacaccag cgttggggca
    15961 gcaaaaactt tatgttctgg tctttaccac ggaaaaagat ctcctgcaga cgacccaact
    16021 gctcgacccg gctaaagcct atgccaaagg cgtcggtaac tcgatcccgg atattcctga
    16081 cccggttgct cgtcatacca ccgatggctt actgaaactg aaagtcaaaa cgaactccag
    16141 ttccagcgtg ttggtcgggc ctctatttgg ttcttccgct ccagctccgg ttacggtagg
    16201 caacacggcg gcaccagctg tggctgcacc cgctccggca ccggtgaaga aaagcgagcc
    16261 gatgctcaac gacacggaaa gttattttaa taccgcgatc aaaaacgctg tcgcgaaagg
    16321 tgatgttgat aaggcgttaa aactgcttga tgaagctgaa cgcctggggt caacatctgc
    16381 ccgttccacc tttatcagca gtgtaaaagg caaggggtaa ttacgcccca cagtgctgat
    16441 tttgcaacaa ctggtgcgtc tcctggcgca ccttttttta tgcttcattc ctgggatatg
    16501 agcgattttt tatagtactt cgcttcttct tcactaagaa tatccattat ctcaatgcct
    16561 tatcagagat tcttttcctt tcgccggtag tgtctggacg ctcaggctac ttttccagtt
    16621 atttcatttc tgtaatacag aggttttatg ataggtcata tcacaaattc tggcggcaat
    16681 gactctttga tgaaacatga tgtggtgcaa ggaaataata aattagatct tgatttacta
    16741 cgtaatttta atggggtgcc aggtttaaat agagataact ttatttatat cagtgatata
    16801 tttttaaata taaaacaacg gaacgaaaaa aatcatgcaa taaatatgtt tcgtgaagtc
    16861 tcaatcagta atgatattat aagcgtaaaa ttttatagaa atgaagaaat agaatgtgct
    16921 tgtgattttc tgatggataa agatgcgcag gggtatactg acctgtctga tttggattta
    16981 acaagttgtc attttaaagg tgacgttatt tcgaaggtgt ctttcatatc atcaaatcta
    17041 caacatgtaa cattcgaatg taaagaaata ggggattgca attttactac tgcaacagtt
    17101 gataatgtca tatttaaatg tcgacgttta cacaatgtga tttttatcaa agcgactggc
    17161 gaatatgtcg attttagcca aagtattctt gatacagttg acttctcgcg gagccagctt
    17221 actcatagta attttcgtga atgtcagatt agaaattcaa agttcaataa ttgttatctt
    17281 tatgcttcgc acttcaccag agcagaattt ctttctacca aagaaatatc atttattaaa
    17341 tcgaatctga cagctgttat gtttgatcat gtgcgaatgt cgacagggaa ttttaaagat
    17401 tgcattacag aacgattgga attaactatt gattattcag atatatttgg gaatgaagaa
    17461 cttgatggtt atatcaataa cattataaaa atgattgata cattgccaga taatgcaatg
    17521 atattgaaat ccgttctggc agtaaaactg gtgatgcaat taaaaattct taatattgtt
    17581 aataaaaact ttattgagaa tatgaagaaa atatttagcc attgtcctta tataaaagat
    17641 ccaattatac gtagttatat ccatcctgat gaagataaca agttcgataa ttttatgcgt
    17701 caaaatcgat tcagtaagat gcatttcgat acccaacaga tgatcgattt tattaacaga
    17761 tttaatatga ataaatggct gattgatcaa aataacaatt tttttatcca acttatcgat
    17821 caggctctac gatcaacgga tgatacgatc aaagcaaatg cctggcatct ttataaagag
    17881 tggattcgta gtgatgatgt ttcaccttta tttatagaaa ttgaagataa tttaagaacc
    17941 tttaacacga atgaattaac acgaaatgat aatatcttta tcctgttctc ctctgtcgat
    18001 gatgggccag ttatggtggt aagctcccag cgcttacatg atatgttgaa tcctacaaaa
    18061 gataccaatt ggaattccac gtatatctac aaatccagac atgagatgtt gcctgttaat
    18121 cttactccgg aaacactttt cggctccaaa tctcaggata aacatgcgct tttccccatt
    18181 tttactgcga gttggcgagc taatcgtata aagaataaag gtatttaagt aaaggaaaac
    18241 atcaccgttc ctggcatcct gggcggtgat gcccctatgg ttgacctcgc cagcacgggc
    18301 atcggcaaag cgtaaggttc aacagcgttt taccacttca tgcgattgtt gcgtttttgt
    18361 tgcgcattag atcacttaat ttgctttaca tctcccgtaa acacttttct gcgatacaat
    18421 gcctttacgt tatgtaacgg agagttcggc atgtcacacc ccgcgttaac gcaactgcgt
    18481 gcgctgcgct attttaaaga gatccctgcc ctggatccgc aactgctcga ctggctgttg
    18541 ctggaagatt ccatgacaaa acgttttgaa cagcagggaa aaacggtaag cgtgacgatg
    18601 atccgcgaag ggtttgtcga gcagaatgaa atccccgaag aactgccgct gctgccgaaa
    18661 gagtctcgtt actggttacg tgaaattttg ttatgtgccg atggtgaacc gtggcttgcc
    18721 ggtcgtaccg tcgttcctgt gtcaacgtta agcgggccgg agctggcgtt acaaaaattg
    18781 ggtaaaacgc cgttaggacg ctatctgttc acatcatcga cattaacccg ggactttatt
    18841 gagataggcc gtgatgccgg actgtggggg cgacgttccc gcctgcgatt aagcggtaaa
    18901 ccgctgttgc taacagaact gtttttaccg gcgtcaccgt tgtactaaga ggaaaaaaat
    18961 atggagtgga gtctgacgca gaataagctg ctggcgtttc atcgcttaat gcgtacggat
    19021 aagccaattg gcgcgttact gctgctctgg ccaacattat gggcgttgtg ggtggcgaca
    19081 ccgggcgttc cccagctctg gatcctggcg gtgtttgtcg cgggtgtctg gctgatgcgc
    19141 gctgccggat gtgtggtgaa tgattatgct gaccgcaagt ttgatggtca tgttaagcgc
    19201 acggcgaacc gaccacttcc cagcggtgcg gtaacagaga aagaggcgcg cgcgctgttt
    19261 gtagtgctgg tactgatttc gtttttactg gtgctgacgc tgaatacgat gaccattcta
    19321 ttgtcgattg ccgcgctggc gctggcgtgg gtgtacccgt ttatgaagcg gtatacccat
    19381 ctaccgcaag tggtgctggg cgcggcgttt ggctggtcga ttccaatggc ttttgctgct
    19441 gtgagtgagt cggtgccatt gagttgctgg ttaatgttcc tcgccaatat tctctgggcg
    19501 gtggcatacg acacgcagta tgcgatggtt gaccgtgatg atgatgtgaa gattggcatt
    19561 aaatccaccg caatcctgtt cggccaatac gataaattga ttattggtat tttgcagatt
    19621 ggcgtactgg cactgatggc gatcatcggt gagttaaatg gcttaggctg gggatattac
    19681 tggtcaattc tggtggctgg cgcgctgttt gtttatcaac aaaaactgat tgccaaccgc
    19741 gagcgtgaag cctgctttaa agcgtttatg aataataact atgttggtct ggtactgttt
    19801 ttagggctgg caatgagtta ctggcatttc tgatgatgta aaaaagccgg atgatcatcc
    19861 ggctttcttc tgggctgcct gatgcgctgc gcttctcagg cctacacaac agatcgcaat
    19921 ttattgaatt tgcagatcat ggaaggccgg ataaggcgtt tgagccgcat ccggcaattc
    19981 tcactgatta cccctcgccc tgcgtcgcac tctcaatcgt caaacgcacg tctgatgtaa
    20041 tcaactccgc cagcaactga taaaccttca tcgtttctgc cggttcggca tcgccgctat
    20101 cgctgatata cccttcatca cgcagcgtca gcaccagcga actgaacacc gctttgtcga
    20161 agaactccgg cgcgttgata ccgtgcagca cggagagacg ttgcgcgacg gtgcggctct
    20221 ctttttccag cgtaccgcgg ttgatcgacg ggttggcgct caacagccag aaggtaatgg
    20281 cataacgttg cagcgtttcg cgcgcgcctg cggccagcag ctgtagcgtg cgagaatgcg
    20341 ccgggttgat atgcaactca tcatcttgca gggtaatcag cccctgacgt tgcatctcat
    20401 ttgccagcgc atcaataacg tctggcaatt cgtcgcgatc ccagcgcagg aacagctccg
    20461 ctttcagcat tgggtaaagc acattgacgt gctccatcaa tacgtcgcgg gagatgtggc
    20521 gatgctgagt gacaattgcc gccatcagcg aaggcagcac caacatatgc gcaatgttgt
    20581 tgcgatagta ggtcatcaac accgcttgct cgcgcggcag aatgatgatg tcgccgatag
    20641 tgtctttctc gacttcaaac ttgttcattt gcagcgcgtg atcgataagc tcgctggcgc
    20701 tggctgaagg aacggtagag tccgtggagt aaggtacgtt gcgcatcaga tccaggtagc
    20761 agttgagttg ctcggttaac tgctcgcggg tgagtgagcg ctgacgtgac gccagtagcg
    20821 cagtacagca caggttcatg gcgtttgccg cgcctgcgtt gttaatgcgt accatcagat
    20881 cggcagcaat attattgacc gtcggcgtta accaggccgg acgcaccgct tcgatgggat
    20941 cgatagattc acgccagtct ggtacgtgct ggttaaggta ggtcatcaac ggcattggtt
    21001 caccgaagtt gacgtaaccc tgaccgagat tacgcagctt gcttaaaccg cgcagcatct
    21061 gcggcaggct ctctttctct ttcgttgcac cgcgcagttc tttggcgtaa gtacccactt
    21121 ccatgacgtg ctcataaccg atatagatcg gaatcagcgt aatcggacgc gtgccgccac
    21181 gcagcatcgc ctgaatggtc atcgacagcg taccggtttt cggatccagc aaacgccccg
    21241 tacgggaacg accgccttcc acgaaatact cgacggaata accacggctg aacagttcgc
    21301 cgagatactc acggaaaacg gtggaataga gcttattgcc tttaaacgta cggcgaataa
    21361 agaacgcacc cagacggcgg aaaatcggcc cggccggcca gaagttcagg ttgatcccgg
    21421 cggcgatatg cggcggcacc agcccctggt gatacagcac gtaagaaagc agcaggtagt
    21481 ccatgtgact gcggtggcaa ggcacataca ccagctcatg accgtcgtgg gccaactggc
    21541 gaacgcgctc ggcgttatgg acgttgatac cctggtaaag gcggttccag gtgaagccca
    21601 gaatgcggtc agtcaggcgg atcatctcgt aagagaaatt cgccgcaatc tcttccatca
    21661 gcacaatcgc gttctgctgc gctttttcat gggagatttt tttgctgcgc gcttcatctt
    21721 ctaccgcttt ggcaatggcg cgggaggcga gcagcttatt aaacagatcc tgacgagcag
    21781 gcagacgtgg gcctacggca gccagacgtt gacgggcaaa gtgcatacgc gccacgcgcg
    21841 ccagtttctg cgcgatagtt ttatccgtgc cgtgttcatc cgccatacgg cgcagcgaaa
    21901 ctgacggcga gaaacgcaca aaactgtcgc gaccgagcca cagtacagcg aaaaatttct
    21961 gtacgccgtt aagcatacgc agcggcgggt tcacttcgcc tttttcacgc cccggcgcgc
    22021 gaccaaacat caccgatact ggcaccatct gcacatccag atttgggttg ctacggtgca
    22081 aatcgagata gtcgtggaac agcttaatag actcttcttt cggcgtgtaa taggtgaaca
    22141 cacgcggccc gccgtgaatg aacacatagc gcggcagtag cgtgccgtcg atttccagcg
    22201 gctctaacgg gtcaggcagg tcatgtgcca gacactgggc gcgcaacgtc agcaagtcag
    22261 ctttcgagtt gtacggtaaa acgtacataa ttggacgaga ggtatccagc cccagttccg
    22321 gggcaggatc tgccggaata gacttgcttt ttaccaggat gcttaatggt aaattcagta
    22381 atttgtagta aattcgtggc cagccggaca taaacgatgt aaagcctctg gttaataatg
    22441 caaatgcgcg gcaaggatag cagaaagtca tgggaaattc tgtggtatcc gctcatgttt
    22501 cgcgcggcgt tacgcaaacc cgaatcatcg gatttaacgg tacactgata ttgacgctca
    22561 taatgtaaaa aggttctttc aatggccaat aataccactg gattcacccg aattatcaaa
    22621 gctgctggct attcctggaa aggtttacgc gctgcatgga tcaacgaagc ggcattccgt
    22681 caggaaggcg tagcggtatt gttggcggtg gtcatcgcct gctggctgga tgtggacgca
    22741 attacccgcg tgctgcttat cagctccgtg atgctggtga tgattgtgga aatcctcaat
    22801 agcgccatcg aagcagtggt tgaccgaatt ggctctgaat accatgagct atccggacgc
    22861 gctaaagata tgggatccgc cgcggttctg attgcgatta tcgtcgccgt gattacctgg
    22921 tgcatcctgt tatggtcgca ttttggataa cccttccaga tttcgataaa tctctggttt
    22981 attgtgcagt ttatggttcc aaaatcgcct tttgctgtat atactcacag cataactgta
    23041 tatacaccca gggggcggaa tgaaagcgtt aacggccagg caacaagagg tgtttgatct
    23101 catccgtgat cacatcagcc agacaggtat gccgccgacg cgtgcggaaa tcgcgcagcg
    23161 tttggggttc cgttccccaa acgcggctga agaacatctg aaggcgctgg cacgcaaagg
    23221 cgttattgaa attgtttccg gcgcatcacg cgggattcgt ctgttgcagg aagaggaaga
    23281 agggttgccg ctggtaggtc gtgtggctgc cggtgaaccg cttctggcgc aacagcatat
    23341 tgaaggtcat tatcaggtcg atccttcctt gttcaagccg aatgctgatt tcctgctgcg
    23401 cgtcagcggg atgtcgatga aagatatcgg cattatggat ggcgacttgc tggcagtgca
    23461 taaaactcag gatgtacgta acggtcaggt cgttgtcgca cgtattgatg acgaagttac
    23521 cgttaagcgc ctgaaaaaac agggcaataa agtcgaactg ttgccagaaa atagcgagtt
    23581 taaaccaatt gtcgttgacc ttcgtcagca gagcttcacc attgaagggc tggcggttgg
    23641 ggttattcgc aacggcgact ggctgtaaca tatctctgag accgcgatgc cgcctggcgt
    23701 cgcggtttgt ttttcatctc tcttcatcag gcttgtctgc atggcattcc tcacttcatc
    23761 tgataaagcg ctctggcatc tcgccttacc catgattttc tccaatatca ccgttccgtt
    23821 gctgggactg gtcgatacgg cggtaattgg tcatcttgat agtccggttt atttgggcgg
    23881 cgtggcggtt ggtgcaacgg cgaccagctt tctctttatg ctgttgctgt ttttacgcat
    23941 gagcaccacc gggctgactg cgcaggctta tggtgccaaa aatcctcagg cattagcccg
    24001 tgcgctggtg caaccgttgc tgttggcgtt gggggctggg gcgttaattg cgctgctgcg
    24061 tacgccgatt atcgatctgg cgctgcatat tgttggcggc agcgaagcgg tgctagaaca
    24121 ggcgcgacgc tttcttgaaa tccgctggtt aagtgcaccg gcgtcgctgg cgaatctggt
    24181 attacttggt tggttactcg gtgtgcaata tgcccgtgcg ccagtaattt tgttagtggt
    24241 cggcaatatc ctcaacattg tgctggatgt ctggctggtg atggggctgc atatgaacgt
    24301 gcagggcgcg gcgctggcga cggttattgc ggaatatgca acattgctga ttggtctgct
    24361 aatggtgcgt aaaatcctca aactacgcgg aatctccggc gaaatgctga aaactgcctg
    24421 gcgaggaaac ttccgtcact tgctggcgct taaccgcgat atcatgctgc gctcgctgtt
    24481 gttgcaactc tgtttcggcg cgatcaccgt acttggcgcg cgactgggga gtgacattat
    24541 cgctgttaac gcggttctga tgacgttact cacctttacc gcctatgcgc tggatggttt
    24601 tgcctacgcg gttgaagcgc attccggtca ggcgtacggt gcgcgcgatg gtagccagtt
    24661 actggatgtc tggcgggcag cgtgccgcca gtcggggatt gtagcgttac tgttttcggt
    24721 ggtttatttg ctggcagggg aacacatcat tgcgttgctg acgtcgttaa cccagattca
    24781 gcagctggct gaccgctatc ttatctggca ggtgattttg ccgttggtcg gcgtctggtg
    24841 ttatctgctc gacggcatgt ttataggtgc aacgcgcgcc gccgaaatgc gtaacagtat
    24901 ggcggtggcc gccgcaggtt ttgcgctgac gctccttacg ctgccgtggc tggggaatca
    24961 tggtttgtgg ctggcattaa ccgtctttct ggcgttacgc gggctttctc tggcggctat
    25021 ctggcggcgt cactggcgca acggtacctg gtttgccgca acgtgacggt taaaaattct
    25081 gaataaataa tcctaagcca aattgctgac tacacttaat ctcacgttca gaagaaaagt
    25141 gaacgtactc tcattcacaa cctaacgatg aggtcttgat tatgaataaa gatgaagccg
    25201 gcggtaactg gaaacagttt aaaggtaaag tgaaagagca atggggcaaa ctgaccgatg
    25261 atgatatgac gatcattgaa ggtaaacgtg atcaactggt cggtaaaatc caggaacgct
    25321 acggttatca gaaagatcag gcagaaaaag aggttgatag ttgggaaaaa cgccatgatt
    25381 accgctggta attaatccct cctgcccgac gtgtacaagg atgtacgccc tcttaacgtg
    25441 atttcttttt cacctgtaca gagtgatcat gctggcactg ttcaggatga cgacacgctt
    25501 ccacttccac gcatgccgca cagagtccat gtgcttcaat cacattatgc cgcagggcaa
    25561 accccatttt tgccgccagc gtatgcataa tgtcttccac gccttctgca cattcttctt
    25621 tcactgcgcc gcagcgatcg caaataaaca tggctgacgt atgcgtgggc tggtcgaaca
    25681 gatgacagag cacataactg ttggtggatt ccaccttatg cacaaaacct tgctcaagca
    25741 gaaaatccag cgcgcgataa accgttggcg gcttggcttg cggttcagct tcgcgcagca
    25801 aatcaagcag atcataagcg ctgatagcgc catcctgcag actcatcagg cgcaacactt
    25861 ccaggcgctg tggggtcagg cgcacattac gctgcgcgca gattttttca gcctgcgcta
    25921 ataactcctg cgttgtggtc ttttccatcg ggcacctcaa agtgggggtt aagaatgtcc
    25981 ttactttacc atgttccagg aaaaacaccg agaaccctta ttgttgccgt aatgttgatt
    26041 ttctgttttg tacgtaaggt gttgtgttgc ctggtagtac cattatcaac acgataataa
    26101 ttaataattc atttttaaat aaattgcttc aagttaactc gctatttatt tagctgggtt
    26161 tgatcgcata cctgtacttc agaaattatg agcgattttt gatagtgagc agatggcaaa
    26221 agaattataa ggaggacacg gtacgacaaa aaaggtgcgt atatcatgtc aggtataatt
    26281 tcaagtgcaa cagaattctt caataataca ctccattcaa atcaatacga aattaaagat
    26341 gcgagcaaag aaaaaataaa ggatatatat aataaatcgg ttgattatgc aacgatgagt
    26401 aatcgcgctg cgattattgc aaaaatagat ttgtttattc agagaatcac ccataattta
    26461 tggactgcaa gtgataataa tgtttcctta attaagcgaa tagatgctct caaaaattgg
    26521 gttgaggagt gtattgatca gtgcactaat gagcaattag gtgatcgtga ctatatttct
    26581 gcatttgtgg atcgggcgat ttttcattac gcgatgaatt ttgtttgtaa tccaatggaa
    26641 aatagcgatg taactctaat agacaagtgt actttcgacg taataaatca taatggcctt
    26701 ccctccacga ttcagctatt ttatgataaa tcctattgta aagcacctgt agcgtcatta
    26761 catctaaaaa cagtaagcga aggcttttta aatttggaaa atgcatataa tgtaaagaaa
    26821 gatactcgct taaaacccct ggcctccctt ttactttcat tttcatatga tagtgcatca
    26881 gcagatatca taggagcagc agagagagtt aatgatgatt gtgaaaacta cataaaaagt
    26941 cagataaaaa tattatctcg tcaagtgatg aaaaaaaacc tggcggagct gaaagacttg
    27001 atagataaaa aagaattgga ttttaatgta attctgcagg agatgaaaaa tcaggccgga
    27061 gaagaaaaag aagacatcaa aaacattaca aaaatgcagg ctgcaggtga gactttaatt
    27121 aaatttattc aatcatttga cccggactca acgggaccaa ttataattgg taaagatatg
    27181 gatgacaaaa atataaatga caacgatcta attaacgaag atattattaa gttcatttat
    27241 gtcctattag gtactgagtt agaaaaataa aatgtgaatt ttccctatag catggaagtt
    27301 tttacataaa agaacccaat attgattggg ttcttttttc tctatcagtg ctattagcag
    27361 ggagagatat caccgtagtt taatgtgtga tttttattta tcgtcgaacc tggattgttt
    27421 atcattggcc ttaacaaagc taacggctaa taagattatt tccatcactt cgtgagagct
    27481 tcatgccttg agaggatctc aattttcttt tgcaatgaga caggcgcttc ctgttgttat
    27541 ggtatagtac cccgctattg agcctcctga acagtgatgc tgaataacat aactcaatga
    27601 tatatcgata aaataatctc tacatttgaa aatgcacggt aattctgaaa tgcaaaaaat
    27661 caaccaaacc agcgcaatgc ctgaaaaaac tgacgttcac tggagtggtc ggtttagcgt
    27721 tgcaccaatg ctcgactgga cggacagaca ttgccgctat ttcttgcgtc tgctttcccg
    27781 caatacgttg ctgtataccg aaatggtgac tacaggggcg attattcacg gtaaaggtga
    27841 ttacctggcg tacagtgaag aagaacatcc ggtagcattg caactgggcg gtagcgatcc
    27901 ggcggcgctg gcgcagtgtg caaagctggc agaagcgcgc ggatatgatg agatcaacct
    27961 gaatgtcggc tgcccgtctg accgggtgca gaacggcatg tttggggcgt gtctgatggg
    28021 taatgcgcag ctggttgccg actgcgtgaa agcgatgcgc gatgtggtgt cgattccggt
    28081 gacggtgaaa acgcgtattg gcattgacga ccaggacagc tatgaatttc tctgcgattt
    28141 catcaacacc gtttccggca aaggcgagtg tgagatgttc atcatccacg cacgtaaagc
    28201 ctggctttcg gggttaagcc cgaaagaaaa ccgtgaaatc ccgccgctcg attatccgcg
    28261 tgtgtatcaa ctgaagcgtg actttccgca tctgacaatg tcgattaacg gtggtatcaa
    28321 gtcgctggaa gaggctaaag cgcatttgca acatatggat ggcgtgatgg tcgggcgcga
    28381 agcgtatcag aatccgggga ttctggcggc ggtagaccga gagatttttg gttcctcgga
    28441 taccgatgcc gatccggtgg cggtagtgcg cgccatgtat ccgtacattg agcgtgaact
    28501 cagccagggg acgtatctcg gccatattac ccggcatatg ctgggcttgt tccagggtat
    28561 tcctggcgcg cggcagtggc ggcgttattt aagtgaaaat gcccataaag cgggtgcaga
    28621 cattaatgtg ctggaacacg cgctcaaact ggtggcggat aagcgttaac ttttcaccaa
    28681 aaagtagtca aattcaccac gccctgcgca ccgtcgcggg gcgttttgct gttaaatcaa
    28741 tagattattt ttggcatgat tcttgtaatg cctgcaagag atttcatatt tgggagagca
    28801 tcatgctgga actacttttt gtgattggct tttttgtcat gctgatggtc accggcgttt
    28861 cgttgctggg cattatcgcc gcgctggttg tggcgacggc cattatgttc ctcggcggta
    28921 tgctggcatt gatgattaag ttgctgccgt ggttactact ggcggttgcg gtggtgtggg
    28981 ttattaaggc gattaaagca ccaaaagtgc cgaaatatca gcgttatgac cgctggcgtt
    29041 actaatggat tgtgcggatg atcacaacct aaggttttat ccttagaaca aaataggaat
    29101 tgataatcaa atctgtcact attgcgcctc taacagattc atcgtgctgt accctacata
    29161 cagccgaact ataaaaagaa agggcttccc aggtggaagc cctatttctt ttatggaatc
    29221 aacaggctgg aaccttgcgt cgcccggctt tccagaatct catgcgcacg ctgcgcatcc
    29281 ttcagcggat atttctgctg ctcggcgaca tcgaccttaa tcacaccgct ggcaatcaaa
    29341 gagaacagtt cattactggc ctcggttaat tcctcccgcg tggtgatata gccttgcagg
    29401 gaagggcgtg tcacatacaa cgagcctttc tgattgagaa tgcctaagtt cacaccggta
    29461 accgcacctg atgagttgcc aaaactcacc attaagccgc ggcgttgcag gcaatccagc
    29521 gaccgttccc aggtgtctct gcccacggaa tcgtacacca cgcgcacttt cttaccagcg
    29581 gtgatctctt tcaatcgctc gaccagattc tcttcacgat agttaataac ctgccacgcg
    29641 ccggctttta gcgcgctctg cgctttttgc gcggttccta cggtgccgat aagtttcgcg
    29701 cccagggctt ttgcccactg gcaggcaatt aagccaacgc cgccagccgc tgcgtggaac
    29761 aggaactgct catcgggttt aatttcatag gttttgcgca gcagataata aaccgttaag
    29821 cctttcagga aggatgccgc cgcttgctca aaagaaattg ccgcaggcag aatcgccgct
    29881 ttatccgcat taatgttatg caccgagctg taagcgccta acgccgactg cgcatagact
    29941 acacgatcgc ctgccttaat atgctttaca ccactgccga ctttactcac gatgcctgcc
    30001 gcttcggtac ctaatccgct ggggagcgat ggcggcgggt aaaggccgct gcggatatac
    30061 gtgtcgataa aattgatgcc gatggcttta ttttcgacct ggatttcatt ctccgccgga
    30121 tcggcaggag tgaactctac ggcttgaagt acttccgggc caccgtgctt gtgaaattca
    30181 attcgtgttg ccatgtgtcc tccagaacgt atcgtcaggg tctgcttcat atggtaaagt
    30241 ttcgacccat tctttatctc ggtaactcca ttcactatgg caggaaataa acccttcaac
    30301 aaacagcagg ctgaaccccg cgaacgcgat ccacaagttg ccgggctgaa agtgcctccg
    30361 cactcgatcg aagcggagca gtcggtgttg ggcggtttaa tgctggataa cgaacgctgg
    30421 gatgatgtag ccgagcgtgt ggtggcagac gacttttaca cccgcccaca ccgtcatatc
    30481 tttactgaaa tggcgcgttt gcaggaaagc ggtagtccta tcgatctgat tacccttgcg
    30541 gaatcgctgg aacgccaggg gcaacttgat agcgtcggtg gtttcgctta tctggcggag
    30601 ctgtcaaaaa atacgccaag tgcggcgaac atcagtgctt atgctgacat cgtgcgtgaa
    30661 cgtgccgttg ttcgcgagat gatttcggtt gcgaatgaga ttgccgaagc cggttttgat
    30721 ccgcaggggc gtaccagcga agatctgctg gacctggctg aatcccgcgt ctttaaaatt
    30781 gccgaaagtc gtgcaaacaa agacgaaggg ccgaagaaca tcgccgatgt gctcgacgcc
    30841 acggtggcgc gtattgagca gttgtttcag cagccacacg atggcgttac cggggtaaac
    30901 accggttatg acgatctcaa caaaaaaacc gctggcttgc agccgtcgga tttgatcatc
    30961 gtcgccgcgc gtccgtcgat gggtaaaaca acatttgcga tgaacctcgt cgaaaacgcg
    31021 gcgatgttgc aggataaacc agtacttatc ttctcgctgg agatgccatc agaacagatt
    31081 atgatgcgtt ctttggcgtc gctgtcgcgc gttgaccaga ctaaaatccg taccgggcag
    31141 ctcgatgatg aagactgggc acgtatttcc ggcaccatgg gtattttgct cgaaaaacgc
    31201 aatatctata tcgatgactc ctccggcctg acgccaacgg aagtgcgttc ccgcgcacgc
    31261 cgtattgccc gtgaacacgg cggcatcggg cttatcatga tcgactacct gcaactgatg
    31321 cgcgtaccgg cgctttccga taaccgtacg ctggaaattg cagaaatctc ccgctcgctg
    31381 aaagcactgg cgaaagaact gaacgtgccg gtggtggcgc tgtcccagtt gaaccgttct
    31441 ctggaacaac gtgccgacaa acgcccggtc aactccgacc tgcgtgaatc tggctctatc
    31501 gagcaggatg cggacttgat catgtttatc tatcgtgatg aggtgtatca cgaaaacagt
    31561 gatttaaaag gcatcgcgga aattattatc ggtaaacaac gtaacggccc aatcgggacg
    31621 gtacgcctga cctttaacgg tcaatggtcg cgcttcgaca actatgcggg gccgcagtac
    31681 gacgacgaat aataattatt ttatgaatta ggtaattaaa gcaaacactt atcaaggaac
    31741 acaaatgcaa gcggcaactg ttgtgattaa ccgccgcgct ctgcgacaca acctgcaacg
    31801 tcttcgtgaa ctggcacctg ccagtaaaat ggttgcggtg gtgaaagcga acgcttatgg
    31861 tcacggtctt cttgagaccg cgcgaacgct ccccgatgct gacgcctttg gcgtagcccg
    31921 tctcgaagaa gctctgcgac tgcgtgcggg gggaatcacc aaacctgtac tgttactcga
    31981 aggctttttt gatgccagag atctgccgac gatttctgcg caacattttc ataccgccgt
    32041 gcataacgaa gaacagctgg ctgcgctgga agaggccagc ctggacgagc cggttaccgt
    32101 ctggatgaaa ctcgataccg gtatgcaccg tctgggcgta aggccggaac aggctgaggc
    32161 gttttatcat cgcctgaccc agtgtaaaaa cgttcgtcag ccggtgaata tcgtcagcca
    32221 ttttgcgcgc gcggatgaac caaaatgcgg cgcaaccgag aaacaactcg ctatctttaa
    32281 taccttttgc gaaggcaaac caggtcaacg ttccattgcc gcatcgggtg gcattctgct
    32341 gtggccacag tcgcattttg actgggtgcg tccgggcatc attctttacg gcgtctcgcc
    32401 gctggaagat cgttccaccg gtgccgattt tggctgtcag ccagtgatgt cactaacctc
    32461 cagcctgatt gccgtgcgtg agcacaaagc cggagagcct gtcggttatg gtggaacctg
    32521 ggtaagcgaa cgtgataccc gcctgggcgt agtcgcgatg ggttatggcg atggttatcc
    32581 gcgcgccgcg ccgtccggta cgccagtgct ggtgaacggt cgcgaagtgc cgattgtcgg
    32641 gcgagtcgcg atggatatga tctgcgtaga cttaggtcca caggcgcagg ataaagccgg
    32701 ggacccggtc attttatggg gcgaaggttt gcccgtagaa cgtatcgctg aaatgacgaa
    32761 agtaagcgct tacgaactta tcacgcgcct gacttcaagg gtcgcgatga aatacgtgga
    32821 ttaatcgttc tgtaatattt gattgtctgt gccggatgcg gcgtgaatgc cttatccggc
    32881 caataaaagc ctaccaattc aataagttga tattctttca tgctcttatg aaggtcgtgc
    32941 ctctggcgga tgtacgtttg tcatgagtct cactctgttg ctaattgccg ttcgcccctg
    33001 aacatccact cgatcttcgc cttcttccgg tttattgtgt tttaaccacc tgcccgtaaa
    33061 cctggagaac catcgcgtgt ttcaaaaagt tgacgcctac gctggcgacc cgattcttac
    33121 gcttatggag cgttttaaag aagaccctcg cagcgacaaa gtgaatttaa gtatcggtct
    33181 gtactacaac gaagagggaa ttattccaca actgaaagcc gtggcggatg cagaagcgcg
    33241 cctgaatgcg cagcctcatg gcgcttcgct ttatttaccg atggaagggc ttaacagcta
    33301 tcgccatgcc attgcgccgc tgctgtttgg tgccgaccat ccggtactgc aacaacagcg
    33361 cgtagcaacc attcaaaccc ttggcggctc aggggcattg aaagtgggcg cagatttcct
    33421 gaaacgctac ttcccggaat caggcgtctg ggtcagcgat cctacatggg aaaaccacgt
    33481 agcaatattc gccggggctg gattcgaagt aagtacttac ccctggtatg acgaagcgac
    33541 taacggcgtg cgctttaatg acctgttggc gacgctgaaa acattacctg cccgcagtat
    33601 tgtgttgctg catccatgtt gccacaaccc aacgggtgcc gatctcacta ataaccagtg
    33661 ggatgcggtg attgaaattc tcaaagcccg cgagcttatc ccatttcttg atattgccta
    33721 tcaaggattt ggtgccggta tggaagagga tgcctacgcc attcgcgcca ttgccagcgc
    33781 tggattaccc gctctggtga gcaattcgtt ctcgaaaatt ttctcccttt acggcgagcg
    33841 cgtcggcgga ctttctgttc tgtgtgaaga tgccgaagct gcagaccgcg tactggggca
    33901 attgaaagca acagttcgcc gcaactactc cagcccgccg aattttggtg cgcaggtggt
    33961 ggcagcggtg ctgaatgacg aggcattgaa agccagctgg ctggcggaag tagaagagat
    34021 gcgtactcgc attttggcaa tgcgtcagga actggtgaag gtattaagca cagagatgcc
    34081 agaacgcaat ttcgattatc tgcttaatca gcgcggcatg ttcagttata ccggtttaag
    34141 tgccgctcag gttgaccgac tacgtgagga gtttggtgtt tacctgatta ctagcgggcg
    34201 catgtgtgtc gccgggttaa atacggcaaa tgtacaacgt gtggcaaagg cgtttgctgc
    34261 ggtgatgtaa tgcaggaaag caggctggag ctacccagcc tgcagtgaaa ttaaactgtc
    34321 gtcgctttca ctcttgcttt atagatgatt tttttgatgc catcgtttta cgtgagggat
    34381 aataaaaggt tgtcagttct tttattgtta agtttatccc aattatctgg aattcctttc
    34441 cctgtttttt cgtgtggagt aataattttt gcacatctaa ccattattaa atttaataat
    34501 aagtatcggg tttgttacga tagaatattt ctatatcctc aagaatatgc tttgccttat
    34561 tgcctgtgac tttctgagat tcaattatag tatcgttaaa ttctaatgtt aaagagaact
    34621 ctttttttcc gctttgtgta agtttaacct tatattgatt accaatatca aataaaaagg
    34681 tcctgtaatg agaataatca cctttaacct cctcggcaaa acagcaaagt gtgcaaatat
    34741 acatacaatc tttcttgcct ctgattttgt taactaaatc aataaatgca tgaaaaactg
    34801 tacttgcaat agaacttgat acttcttctc caggtctaca aaaatctttt gcaattttta
    34861 actgtgtttc tatatataaa aagaaatctc tggcataaga aaaatctttg gaataagaga
    34921 tatctttatt atttagtgca tctagctcaa caggtaaaga tgtcattttg ttgctattca
    34981 catatgatat attcatatca tcatctcctt cgagagtaat aaaattagtc accattgtag
    35041 ggtagggttc tggtcaacca gaaatcatct ttataaactt cgattgtttt tgtaatgctg
    35101 tatcattaag ttcattaaat cgtacagcag ataaatgttc tatcaaattt cgctcatttg
    35161 cagaggattc atcataataa acgtaaaatt aatgtatcct tcattgagga ataaacattt
    35221 ttttacaaaa aaacgacagg aacgtattta ctgggttaaa tataatcatc ctgcttttca
    35281 tcacaaaaac cgcagataat ccttcctttc ccctgctgct ggcgttatgg tcagatggtt
    35341 tttgcaacaa atctcacaat aaaaagtttc aacatactga ctatttaggg aaaaatatgc
    35401 gcaagatcac acaggcaatc agtgccgttt gcttattgtt cgctctaaac agttccgctg
    35461 ttgccctggc ctcatctcct tcgccgctta accctggtac taacgttgcc aggcttgctg
    35521 aacaggcacc aattcattgg gtttcggtcg cacaaattga aaatagcctc gcagggcgtc
    35581 caccaatggc ggtggggttt gatatcgatg acacggtact tttttccagt ccgggcttct
    35641 ggcgcggcaa aaaaaccttc tcgccagaaa gcgaagatta tctgaaaaat cctgtgttct
    35701 gggaaaaaat gaacaatggc tgggatgaat tcagcattcc aaaagaggtc gctcgccagc
    35761 tgattgatat gcatgtacgc cgcggtgacg cgatcttctt tgtgactggt cgtagcccga
    35821 cgaaaacaga aacggtttca aaaacgctgg cggataattt tcatattcct gccaccaaca
    35881 tgaatccggt gatctttgcg ggtgataaac cagggcaaaa tacaaaatcg caatggctgc
    35941 aggataaaaa tatccgaatt ttttatggcg attccgataa cgatattacc gccgcacgcg
    36001 atgtcggcgc tcgtggtatc cgcattctgc gcgcctccaa ctctacctac aaacccttgc
    36061 cacaagcggg tgcgtttggt gaagaggtga tcgtcaattc agaatactga cagagcggga
    36121 gagcgtgaca ctctcccgaa tgctgttttt ttaatcacac ctttatcctt tctctgtctt
    36181 gctgcaaact gattaagaga gttttatcaa ggagcagcac atgtggtatc aaaagacgct
    36241 cacgcttagc gccaaatctc gtgggtttca tctggtaacg gatgaaattc tgaatcagct
    36301 ggctgatatg ccgcgcgtta acatcggttt actgcatctg ttgctgcaac atacctccgc
    36361 ctctctgaca cttaatgaga actgcgatcc caccgtacgc cacgacatgg agcgtttttt
    36421 cctccgcacc gttcccgaca acggaaatta tgagcatgac tatgagggag cagacgatat
    36481 gccttctcat atcaaatcct caatgctggg aacatcgctt gtattgccgg tgcataaagg
    36541 gcgtattcag accggcacct ggcaaggcat ctggctgggg gaacatcgca tccacggcgg
    36601 atcgcgtcgc attatcgcga cactacaagg ggagtaaaaa atgaccattt cggagttgct
    36661 acaatattgc atggcaaaac caggcgcaga acagagcgtg cataatgact ggaaagcgac
    36721 gcagatcaaa gtggaagatg tactgtttgc gatggtgaaa gaagtagaaa atcgcccggc
    36781 ggtttcgctg aaaaccagcc cggagctggc ggagctgcta cgtcagcagc acagcgatgt
    36841 gcgccccagc cgccatctga ataaagcgca ctggagcacg gtttatctcg acggatcgct
    36901 accggattcg caaatctatt atctggtgga tgcgtcttat cagcaggcgg tgaatttgct
    36961 gccggaagaa aaacgtaaat tgctggtgca actctgaaag gaaaaggccg ctcagaaagc
    37021 ggccttaacg attacagcat cggcttgagg aagcgtgccg tatgcgaagc ttcgcactcc
    37081 gcgacggttt ctggcgtacc ggagacgagg atctcgccgc cgccactgcc gccttccggt
    37141 cccaggtcga caatccagtc agcggttttg atcacgtcga gattgtgttc aatcaccaca
    37201 atggtattgc cctgatcgcg cagtttatgc agcacgtcga gcagttgctg aatatcggcg
    37261 aagtgcagac cggtggtcgg ctcgtcgaga atatacagtg tctgcccggt gccgcgtttt
    37321 gacagctcac gcgccagctt cacgcgctgg gcttcaccgc ctgaaagggt ggttgcggac
    37381 tgccccaggc gaatgtacgt cagaccaacg tccatcagcg tttgcagctt acgcgccaga
    37441 gctggcaccg catcaaagaa ctcacgcgcc tcttcgatgg tcatatccag cacttcgtgg
    37501 atggttttgc ctttgtactt aatctccagc gtttcacggt tatagcgttt acctttacac
    37561 tggtcgcacg gtacgtaaat gtccggcagg aagtgcatct ccactttgat cacgccgtcg
    37621 ccctgacagg cttcgcagcg tccgccacgg acgttaaagc tgaaacgtcc cggcgtatag
    37681 ccgcgcgcac gggattccgg tacgcccgca aacagttcgc gcacaggcgt aaacacgccg
    37741 gtataggtcg ctgggttaga acgcggagta cgaccaattg ggctttggtc gatatcgatc
    37801 actttatcga aatgctccag tccctgaata tcgcgatacg gtgccggttc ggcgatggtc
    37861 gcaccattca actggcgttg ggcaatcggg aacagtgtgt cgttaatcag tgtcgattta
    37921 ccggaacctg aaaccactgt gatgcaggta aacagaccga ctggcagcgt tagcgtcacg
    37981 tctttcaggt tattaccgcg tgcacccgtc agcttcagca ctttttccgg attcgccgga
    38041 acgcgtttct tcggcacttc aatcttgcgt ttaccgctca tgtactgtcc ggtcaacgac
    38101 tccggcaccg ccataatcgc ttccagtgga ccttccgcga ccacttcacc gccgtgaaca
    38161 cccgcaccag gaccgatatc gatcacatgg tcagccgcgc gaatcgcgtc ttcgtcgtgc
    38221 tccaccacaa tcacggtatt accgagatcg cgcagatgga taagcgtacc caacaggcgc
    38281 tcgttatcgc gctggtgcag gccgatagac ggctcatcca gcacgtacat aacgccaacc
    38341 aggcccgcac caatctggct cgccagacga atacgctggg cttcaccgcc agaaagcgtt
    38401 tctgccgagc gggaaagcgt caggtaattc aggccgacgt taacgaggaa tttcaaacga
    38461 tcgccgatct ctttaagaat tttttccgca atcttcgccc gctgacctgc gagtttgaga
    38521 ttgttgaaga attccatcgc atgaccaatg ctcatgtcgg agatagcagg cagcggcgta
    38581 ttctcgacat acacgtggcg cgcttcccga cgcagacgcg tcccttcgca gctggcgcac
    38641 gggcgattgc tgatgaactt ggctaattct tcgcgtaccg cgctggactc cgtctcttta
    38701 taacggcgct ccatattgtg cagcacgcct tcaaacggat gacgacgaat ggaggtatcg
    38761 ccacgatcgt tcatgtattt gaattcaatg ttttctttgc cagaaccgta caacaccact
    38821 ttatgcacgt tcgcgctcag gctgccccac ggcgcttcga cgtcgaactt atagtgatct
    38881 gccagcgatt tcagcatctg gaaataatag aagttgcggc gatcccagcc acggatcgca
    38941 ccgccagcca gcgacagttc cggattctgg atcacgcggt caggatcgaa atattgctgc
    39001 acgccaaggc cgtcacaggt cgggcaggcc cctgccgggt tgttaaacga aaacagtcgc
    39061 ggctccagtt cacgcatact gtagccgcaa attgggcagg cgaaattagc ggagaacagc
    39121 aactcttccg ctttcgggtc gtccatatcc gcaactaccg cggtaccacc ggaaagctcc
    39181 agcgcggttt caaacgactc ggcaagacgt tgggtaagat cgtcacgcac cttgaagcga
    39241 tcaaccacca cttcaatggt atgtttcttt tgcagttcca gtttcggcgg atcggaaaga
    39301 tcgcagactt cgccatcaat acgagcacgg atgtaaccct gacttgccag gttctccagc
    39361 gttttggtgt gttcgccttt gcgctcttta atgattggcg cgagcagcat cagacgcttg
    39421 ccttccggct gcgacagcac gttatccacc atctggctga cggtttgcgc cgccagcggg
    39481 acgtcgtggt ccggacagcg cggctcgccg acgcgggcga acaacaaacg caaatagtcg
    39541 tggatttcgg tgattgtccc caccgtagaa cgcgggttat gagacgttga tttctgctca
    39601 attgagatgg caggagaaag cccctcaata tgatcgacgt ccggcttttc catcagtgac
    39661 agaaactgcc gcgcgtaggc ggaaagtgat tcaacgtaac ggcgctgccc ttcggcatat
    39721 aaggtgtcga aagcgagcga ggatttgcca gaacccgaaa gcccggtcac gacaatgagc
    39781 ttgtcgcggg ggataacgag gttgatgttt ttgagattat gggtgcgggc gccccgaact
    39841 tcgatcttat ccattcacct ttcccggatt aaacgctttt ttgcccggtg gcatggtgct
    39901 accggcgatc acaaacggtt aattatgaca caaattgacc tgaatgaata tacagtattg
    39961 gaatgcatta cccggagtgt tgtgtaacaa tgtctggcca ggtttgtttc ccggaaccga
    40021 ggtcacaaca tagtaaaagc gctattggta atggtacaat cgcgcgttta cacttattca
    40081 gaacgatttt ttttcaggag acacgaacat ggccagcaga ggcgtaaaca aggttattct
    40141 cgttggtaat ctgggtcagg acccggaagt acgctacatg ccaaatggtg gcgctgttgc
    40201 caacattacg ctggctactt ccgaatcctg gcgtgataaa gcgaccggcg agatgaaaga
    40261 gcagactgaa tggcaccgcg ttgtgctgtt cggcaaactg gcagaagtag ccagcgaata
    40321 tctgcgtaaa ggttctcagg tttatatcga aggtcagctg cgtacccgca aatggaccga
    40381 tcaatccggt caggatcgct acaccacaga agtcgtggtg aacgttggcg gcaccatgca
    40441 gatgctgggt ggtcgtcagg gtggtggcgc tccggcaggt ggcaatatcg gtggtggtca
    40501 gccgcagggc ggttggggtc agcctcagca gccgcagggt ggcaatcagt tcagcggcgg
    40561 cgcgcagtct cgcccgcagc agtccgctcc ggcagcgccg tctaacgagc cgccgatgga
    40621 ctttgatgac gacattccgt tctgatttgt cattaaaaca ataggttata ttgtgttaag
    40681 gtggatgatt aaagcatctg ccaaccataa aaaagaagcc tccgttatgg aggcttctac
    40741 atatcaagtc aaaatcattg gccattgtgg cggtgtctga ttcatctctt ctatcatcac
    40801 cgactcgatg ttgtgccaga tggcggtgat atccgccatt gtgatgccaa gcaaccccat
    40861 tgcgaaccag caggcagaaa ccacgcccag accgctgctg atcactgcaa gcccgacata
    40921 atcagaccga cgaaaacgga tgttgagtgt gctggccaga aacatcatta cggcactaag
    40981 aagttgccag cgaagaagaa ccacgccagt ggtgagggta gccatcaaac aattcctctg
    41041 aaaagagccg atgccctgga cagcgcggcg ttgttcacgg gaggcgtggt acactctggc
    41101 tatcgcgggg cttgcagaac acaaaaatga aacactcttt ttgtttcatt gattttgtga
    41161 actatatcac aattgattgt ttgttagcca tattaggacg tgacttttat tgctgtacag
    41221 attatgtggt ttttcagggg cattaagggt atatcttccc accgcctctg cattcctgta
    41281 ggaaattagt tttgaatatc aatgaattat ttttattcag gtgacgatta aaaaggtatc
    41341 aatttcaaat caggcaaaag tgctatttat accgtaagat ttatctaaag acgtcgataa
    41401 ccagggttct cgccttgcaa tgaccgggta taaacaggca ggaaattgat agcaatgagt
    41461 catcgtgcac gacaccaatt actggcgttg ccgggcatta tctttttagt tctctttccc
    41521 atcattctgt cgctatggat tgccttcttt tgggcaaaat cagaagtgaa taatcagctc
    41581 cgaacctttg ctcagctggc gttggataaa tctgagctgg tcattcgtca ggcagattta
    41641 gtgagcgatg cagctgaacg ctatcagggg caagtttgca ccccagccca tcaaaagcga
    41701 atgttgaata ttattcgtgg ctatctttat attaatgaat tgatctatgc ccgtgataac
    41761 cattttttat gctcatcgct gatagcgcct gtaaacggct atacgattgc accggccgat
    41821 tataagcgtg aacctaacgt ttctatctat tattaccgcg atacgccttt tttctctggc
    41881 tataaaatga cctatatgca gcggggaaat tatgtggcgg tcatcaaccc tctcttctgg
    41941 agtgaagtga tgtctgatga tccgacattg caatggggtg tatatgatac ggtaacgaaa
    42001 acctttttct cgttaagcaa cgaggcctcg gcagcaacgt tttctccact gattcatttg
    42061 aaggatttaa ctgtacaaag aaatggctat ttatatgcga cagtttattc gacaaaacgc
    42121 ccaattgcgg ccattgttgc gacttcatat caacgtctta tagcgcattt ttataatcat
    42181 cttatttttg cgttacccgc cggtattttg gggagtcttg ttctgctatt actctggcta
    42241 cgtattcgac aaaactattt gtctcccaaa cgcaaattgc aacgcgccct cgaaaaacat
    42301 caactttgcc tttattacca gccaataatc gatatcaaaa cagaaaaatg tatcggggct
    42361 gaagcattgt tacgttggcc tggtgagcag gggcaagtaa tgaatccggc agagtttatt
    42421 ccgctggcag aaaaggaggg gatgatcgga caggtaactg attatgttat tgataatgtc
    42481 ttccgcgatc tgggcgcata cctggcaaca catgccgatc gctatgtttc tattaacctg
    42541 tcggcctccg attttcatac gtcacggttg atagcgcgaa ccaatcagaa aacagagcaa
    42601 tacgcggtgc gtccacagca aattaaattt gaagtgactg aacatgcatt tcttgatgtc
    42661 gacaaaatga caccaattat tctggctttc cgccaggctg gttacgaagt ggcaattgat
    42721 gattttggta ttggctactc taacttgcat aaccttaaat cattgaatgt cgatattttg
    42781 aaaatcgata aatc
//
LOCUS       DAMZFT010000023        42606 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.023, whole genome shotgun sequence.
ACCESSION   DAMZFT010000023 DAMZFT010000000
VERSION     DAMZFT010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 42606)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42606)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42606
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.023"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..112
                     /gene="rrf"
                     /locus_tag="PN977_001421"
     rRNA            <1..112
                     /gene="rrf"
                     /locus_tag="PN977_001421"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(176..934)
                     /locus_tag="PN977_001422"
     CDS             complement(176..934)
                     /locus_tag="PN977_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HDI6041472.1"
                     /translation="MSQILLQPANAMITLENVNKWYGQFHVLKNINLTVQPGERIVLC
                     GPSGSGKSTTIRCINHLEEHQQGQIVVDGIELNEDIRNIERVRQEVGMVFQHFNLFPH
                     LTVLQNCTLAPIWVRKMPKKEAEALAMHYLERVRIAEHAHKFPGQISGGQQQRVAIAR
                     SLCMKPKIMLFDEPTSALDPEMVKEVLDTMIGLAQSGMTMLCVTHEMGFARTVADRVI
                     FMDRGEIVEQATPDEFFAHPKSERTRAFLSQVIH"
     gene            complement(942..2045)
                     /locus_tag="PN977_001423"
     CDS             complement(942..2045)
                     /locus_tag="PN977_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HDI6041473.1"
                     /translation="MTKVLLSHPPRPASHNSSRAMVWVRKNLFSSLSNSLLTIGCIWL
                     MWELIPPLLNWAFLQANWVGSTRADCTKAGACWVFIHERFGQFMYGLYPHDQRWRINL
                     ALLIGLVSIAPMFWKILPYRGRYIAVWAVIYPLIVWWLMYGGFLGLERVETRQWGGLT
                     LTLIIASVGIAGALPWGILLALGRRSHMPIVRILSVIFIEFWRGVPLITVLFMSSVML
                     PLFMAEGTSIDKLIRALVGVILFQSAYVAEVVRGGLQALPKGQYEAAESLALGYWKTQ
                     GLVILPQALKLVIPGLVNTIIALFKDTSLVIIIGLFDLFSSVQQATVDPAWLGMSTEG
                     YVFAALIYWIFCFSMSRYSQHLEKRFNTGRTPH"
     gene            complement(2055..3236)
                     /locus_tag="PN977_001424"
     CDS             complement(2055..3236)
                     /locus_tag="PN977_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HDI6041474.1"
                     /translation="MSHRRSTVKGSLSFANPTVRAWLFQILAVVAVVGIVGWLFHNTV
                     TNLSNRGITSGFAFLDRGAGFGIVQHLIDYQQGDTYGRVFIVGLLNTLLVSALCIVFA
                     SVLGFFIGLARLSDNWLLRKLSTIYIEIFRNIPPLLQIFFWYFAVLRNLPGPRQAVSA
                     FDLAFLSNRGLYIPSPQLGDGFIAFILAVVMAIVLSVGLFRFNKTYQIKTGQLRRTWP
                     IAAVLIIGLPLLAQWLFGAALHWDVPALRGFNFRGGMVLIPELAALTLALSVYTSAFI
                     AEIIRAGIQAVPYGQHEAARSLGLPNPVTLRQVIIPQALRVIIPPLTSQYLNIVKNSS
                     LAAAIGYPDMVSLFAGTVLNQTGQAIETIAMTMSVYLIISLTISLLMNIYNRRIAIVE
                     R"
     gene            complement(3304..4329)
                     /locus_tag="PN977_001425"
     CDS             complement(3304..4329)
                     /locus_tag="PN977_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HDI6041475.1"
                     /translation="MKKMMIATLAAASVLLAVANQAHAGATLDAVQKKGFVQCGISDG
                     LPGFSYADADGKFSGIDVDICRGVAAAVFGDDTKVKYTPLTAKERFTALQSGEVDLLS
                     RNTTWTSSRDAGMGMAFTGVTYYDGIGFLTHDKAGLKSAKELDGATVCIQAGTDTELN
                     VADYFKANNMKYTPVTFDRSDESAKALESGRCDTLASDQSQLYALRIKLSNPAEWIVL
                     PEVISKEPLGPVVRRGDDEWFSIVRWTLFAMLNAEEMGINSQNVDEKAANPATPDMAH
                     LLGKEGDYGKDLKLDNKWAYNIIKQVGNYSEIFERNVGSESPLKIKRGQNNLWNNGGI
                     QYAPPVR"
     gene            complement(4760..4981)
                     /locus_tag="PN977_001426"
     CDS             complement(4760..4981)
                     /locus_tag="PN977_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6041476.1"
                     /translation="MKRLIPVALLTALLAGCAHDSPCVPVYDDQGRLVHTNTCMKGTT
                     QDNWETAGAIAGGAAAVAGLTMGIIALSK"
     gene            complement(5234..8331)
                     /gene="acrF"
                     /locus_tag="PN977_001427"
                     /pseudo
     CDS             complement(5234..8331)
                     /gene="acrF"
                     /locus_tag="PN977_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417732.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit AcrF"
     gene            complement(8343..9500)
                     /gene="acrE"
                     /locus_tag="PN977_001428"
     CDS             complement(8343..9500)
                     /gene="acrE"
                     /locus_tag="PN977_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit AcrE"
                     /protein_id="HDI6041477.1"
                     /translation="MTKHARFFLLPSFILISAALIAGCNDKGEEKAHVGEPQVTVHIV
                     KTAPLEVKTELPGRTNAYRIAEVRPQVSGIVLSRNFTEGSDVQAGQSLYQIDPATYQA
                     SYDSAKGELAKSEAAAAIAHLTVKRYVPLVGTKYISQQEYDQAIADARQADAAVIAAK
                     ATVESARINLAYTKVTAPISGRIGKSTVTEGALVTNGQTTELATVQQLDPIYVDVTQS
                     SNDFMRLKQSVEQGNLHKENATSNVELVMENGQTYPLKGTLQFSDVTVDESTGSITLR
                     AVFPNPQHTLLPGMFVRARIDEGVQSDAILIPQQGVSRTPRGDATVLIVNDKSQVEAR
                     PVVASQAIGDKWLISEGLKSGDQVIVSGLQKARPGEQVKATTDTPADSASK"
     gene            9899..10561
                     /gene="acrS"
                     /locus_tag="PN977_001429"
     CDS             9899..10561
                     /gene="acrS"
                     /locus_tag="PN977_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor AcrS"
                     /protein_id="HDI6041478.1"
                     /translation="MAKRTKAEALKTRQELIETAIAQFAQHGVSKTTLNDIADAANVT
                     RGAIYWHFENKTQLFNEMWLQQPSLRELIQEHLTAGLEHDPFQQLREKLIVGLQYIAK
                     IPRQQALLKILYHKCEFNDEMLAEGVIRDKMGFNPQTLHEVLQACQQQGCIANNLDLD
                     VVMIIIDGAFSGIVQNWLMNMAGYDLYKQAPALVDNVLRMFMPDENITKLIHQTNELS
                     VI"
     gene            complement(10564..10743)
                     /locus_tag="PN977_001430"
     CDS             complement(10564..10743)
                     /locus_tag="PN977_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2556 family protein"
                     /protein_id="HDI6041479.1"
                     /translation="MIRKYWWLVVFAVFVFLFDTLLMQWIELLATETDKCRNMNSVNP
                     LKLVNCDELNFQDRM"
     gene            complement(10827..11711)
                     /gene="yhdJ"
                     /locus_tag="PN977_001431"
     CDS             complement(10827..11711)
                     /gene="yhdJ"
                     /locus_tag="PN977_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417728.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HDI6041480.1"
                     /translation="MRTGCEPTRFGNEAKTIIQGDALTELKKIPADSVDLIFADPPYN
                     IGKNFDGLIEAWKEDLFIDWLFEVIAECHRVLKKQGSMYIMNSTENMPFIDLQCRKLF
                     TIKSRIVWSYDSSGVQAKKHYGSMYEPILMMVKDAKNYTFNGDAILVEAKTGSQRALI
                     DYRKNPPQPYNHQKVPGNVWDFPRVRYLMDEYENHPTQKPEALLKRIILASSNPGDIV
                     LDPFAGSFTTGAVAAASGRKFIGIEINSEYIKMGLRRLDVASHYSAEELAKVKKRKTG
                     NLSKRSRLSEVDPDLIAK"
     gene            complement(11797..12093)
                     /gene="fis"
                     /locus_tag="PN977_001432"
     CDS             complement(11797..12093)
                     /gene="fis"
                     /locus_tag="PN977_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001465625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator Fis"
                     /protein_id="HDI6041481.1"
                     /translation="MFEQRVNSDVLTVSTVNSQDQVTQKPLRDSVKQALKNYFAQLNG
                     QDVNDLYELVLAEVEQPLLDMVMQYTRGNQTRAALMMGINRGTLRKKLKKYGMN"
     gene            complement(12119..13084)
                     /gene="dusB"
                     /locus_tag="PN977_001433"
     CDS             complement(12119..13084)
                     /gene="dusB"
                     /locus_tag="PN977_001433"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HDI6041482.1"
                     /translation="MRIGQYQLRNRLIAAPMAGITDRPFRTLCYEMGAGLTVSEMMSS
                     NPQVWESDKSRLRMVHIDEPGIRTVQIAGSDPKEMADAARINVESGAQIIDINMGCPA
                     KKVNRKLAGSALLQYPDVVKSILTEVVNAVDVPVTLKIRTGWAPEHRNCEEIAQLAED
                     CGIQALTIHGRTRACLFNGEAEYDSIRAVKQKVSIPVIANGDITDPLKARAVLDYTGA
                     DALMIGRAAQGRPWIFREIQHYLDTGELLPPLPLAEVKRLLCAHVRELHDFYGPAKGY
                     RIARKHVSWYLQEHAPNDQFRRTFNAIEDASEQLEALEAYFENFA"
     gene            complement(13413..14294)
                     /gene="prmA"
                     /locus_tag="PN977_001434"
     CDS             complement(13413..14294)
                     /gene="prmA"
                     /locus_tag="PN977_001434"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HDI6041483.1"
                     /translation="MPWIQLKLNTTGANAEDLSDALMEAGAVSITFQDTHDTPVFEPL
                     PGETRLWGDTDVIGLFDAETDMNDVVAILENHPLLGAGFAHKIEQLEDKDWEREWMDN
                     FHPMRFGERLWICPSWRDVPDENAVNVMLDPGLAFGTGTHPTTSLCLQWLDSLDLTGK
                     TVIDFGCGSGILAIAALKLGAAKAIGIDIDPQAIQASRDNAERNGVSDRLELYLPKDQ
                     PEAMKADVVVANILAGPLRELAPLISVLPVSGGLLGLSGILASQAESVCEAYADSFAL
                     DPVVEKEEWCRITGRKN"
     gene            complement(14306..15757)
                     /gene="panF"
                     /locus_tag="PN977_001435"
     CDS             complement(14306..15757)
                     /gene="panF"
                     /locus_tag="PN977_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/pantothenate symporter"
                     /protein_id="HDI6041484.1"
                     /translation="MQLEVILPLVAYLVVVFGISVYAMRKRNTGTFLNEYFLGSRSMG
                     GIVLAMTLTATYISASSFIGGPGAAYKYGLGWVLLAMIQLPAVWLSLGILGKKFAILA
                     RRYNAVTLNDMLFARYQSRLLVWLASLSLLVAFVGAMTVQFIGGARLLETAAGIPYET
                     GLLIFGISIALYTAFGGFRASVLNDTMQGLVMLIGTIVLLIGVVHAAGGLSNAVETLQ
                     TIDPQLVTPQGADDILSPAFMTSFWVLVCFGVIGLPHTAVRCISYKDSKAVHRGIIIG
                     TIVVAVLMFGMHLAGALGRAVIPDLTVPDLVIPTLMVKVLPPFAAGIFLAAPMAAIMS
                     TINAQLLQSSATIIKDLYLNIRPDQMQNETRLKRMSAVITLVLGALLLLAAWKPPEMI
                     IWLNLLAFGGLEAVFLWPLVLGLYWERANAKGALSAMIVGGVLYAVLATLNIQYLGFH
                     PIVPSLLLSLLAFMVGNRFGTSVPQATVLTTDK"
     gene            complement(15747..15989)
                     /locus_tag="PN977_001436"
     CDS             complement(15747..15989)
                     /locus_tag="PN977_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhdT family protein"
                     /protein_id="HDI6041485.1"
                     /translation="MDTRFVQAHKEARWALGLTLLYLAVWLVAAYLPGVAPGFTGFPR
                     WFEMACILTPLLFIGLCWAMVKFIYRDIPLEDDDAA"
     gene            complement(16098..17447)
                     /gene="accC"
                     /locus_tag="PN977_001437"
     CDS             complement(16098..17447)
                     /gene="accC"
                     /locus_tag="PN977_001437"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009483995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HDI6041486.1"
                     /translation="MLDKIVIANRGEIALRILRACKELGIKTVAVHSSADRDLKHVLL
                     ADETVCIGPAPSVKSYLNIPAIISAAEITGAVAIHPGYGFLSENANFAEQVERSGFIF
                     IGPKAETIRLMGDKVSAIAAMKKAGVPCVPGSDGPLGDDMDKNRAIAKRIGYPVIIKA
                     SGGGGGRGMRVVRGDAELAQSISMTRAEAKAAFSNDMVYMEKYLENPRHVEIQVLADG
                     QGNAIYLAERDCSMQRRHQKVVEEAPAPGITPELRRYIGERCAKACVDIGYRGAGTFE
                     FLFENGEFYFIEMNTRIQVEHPVTEMITGVDLIKEQLRIAAGQPLSIKQEEVHVRGHA
                     VECRINAEDPNTFLPSPGKITRFHAPGGFGVRWESHIYAGYTVPPYYDSMIGKLICYG
                     ENRDVAIARMKNALQELIIDGIKTNVDLQIRIMNDENFQHGGTNIHYLEKKLGLQEK"
     gene            complement(17458..17928)
                     /gene="accB"
                     /locus_tag="PN977_001438"
                     /gene_synonym="fabE"
     CDS             complement(17458..17928)
                     /gene="accB"
                     /locus_tag="PN977_001438"
                     /gene_synonym="fabE"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HDI6041487.1"
                     /translation="MDIRKIKKLIELVEESGISELEISEGEESVRISRAAPAASFPVM
                     QQAYAAPMMQQPAQSNAAAPATVPSMEAPAAAEISGHIVRSPMVGTFYRTPSPDAKAF
                     IEVGQKVNVGDTLCIVEAMKMMNQIEADKSGTVKAILVESGQPVEFDEPLVVIE"
     gene            complement(18794..19623)
                     /locus_tag="PN977_001439"
                     /pseudo
     CDS             complement(18794..19623)
                     /locus_tag="PN977_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000838295.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(19660..20634)
                     /locus_tag="PN977_001440"
     CDS             complement(19660..20634)
                     /locus_tag="PN977_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HDI6041488.1"
                     /translation="MQALLLEQQDGKTLASVQTLDESRLPEGDVTVDVHWSSLNYKDA
                     LAITGKGKIIRNFPMIPGIDFAGTVRTSEDPRFHAGQEVLLTGWGVGENHWGGLAEQA
                     RVKGDWLVAMPQGLDARKAMIIGTAGFTAMLCVMALEDAGVRPQDGEIVVTGASGGVG
                     STAVALLHKLGYQVVAVSGRESTHEYLKSLGASRILPRDEFAESRPLEKQVWAGAIDT
                     VGDKVLAKVLAQMNYGGCVAACGLAGGFTLPTTVMPFILRNVRLQGVDSVMTPPARRA
                     QAWQRLVADLPESFYTQAAKEISLAEAPKFAEAIINNQIQGRTLVKVN"
     gene            20786..22726
                     /gene="csrD"
                     /locus_tag="PN977_001441"
     CDS             20786..22726
                     /gene="csrD"
                     /locus_tag="PN977_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase E specificity factor CsrD"
                     /protein_id="HDI6041489.1"
                     /translation="MRLTTKFSAFVTLLTGLTIFVTLLGCSLSFYNAIQYKFSHRVQA
                     VATAIDTHLVSNDFSTLRPQITELMMSADIVRVDLLHGDKQVYTLARNGSYRPVGTND
                     LFRELSVPLIKHPGMSLRLVYQDPMGNYFHSLMTTAPLTGAIGFIILMLFLAVRWLQR
                     QLAGQELLETRATRILNGERGSNVLGTIYEWPPRTSSALDTLLREIQNAREQHSRLDT
                     LIRSYAAQDMKTGLNNRLFFDNQLATLLEDQEKVGTHGIVMMIRLPDFNMLSDTWGHS
                     QVEEQFFSLTNLLSTFMMRYPGALLARYHRSDFAALLPHRTLKEAESIASQLIKAVDT
                     LPNNKMLDRDDMIHIGICAWRSGQDTEQVMEHAESATRNAGLQGGNSWAIYDDSLPEK
                     GRGNVRWRTLIEQMLSRGGPRLYQKPAVTREGQVHHRELMCRIFDGNEEVSSAEYMPM
                     VLQFGLSEEYDRLQISRLIPLLRYWPEENLAIQVTVESLIRPRFQRWLRDTLMQCERS
                     QRKRIIIELAEADVGQHISRLQPVIRLVNALGVRVAVNQAGLTLVSTSWIKELNVELL
                     KLHPGLVRNIEKRTENQLLVQSLVEACSGTSTQVYATGVRSRSEWQTLIQRGVTGGQG
                     DFFASSQPLDTNVKKYSQRYSV"
     gene            23031..24074
                     /gene="mreB"
                     /locus_tag="PN977_001442"
     CDS             23031..24074
                     /gene="mreB"
                     /locus_tag="PN977_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000913396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreB"
                     /protein_id="HDI6041490.1"
                     /translation="MLKKFRGMFSNDLSIDLGTANTLIYVKGQGIVLNEPSVVAIRQD
                     RAGSPKSVAAVGHDAKQMLGRTPGNIAAIRPMKDGVIADFFVTEKMLQHFIKQVHSNS
                     FMRPSPRVLVCVPVGATQVERRAIRESAQGAGAREVFLIEEPMAAAIGAGLPVSEATG
                     SMVVDIGGGTTEVAVISLNGVVYSSSVRIGGDRFDEAIINYVRRNYGSLIGEATAERI
                     KHEIGSAYPGDEVREIEVRGRNLAEGVPRGFTLNSNEILEALQEPLTGIVSAVMVALE
                     QCPPELASDISERGMVLTGGGALLRNLDRLLMEETGIPVVVAEDPLTCVARGGGKALE
                     MIDMHGGDLFSEE"
     gene            24140..25243
                     /gene="mreC"
                     /locus_tag="PN977_001443"
     CDS             24140..25243
                     /gene="mreC"
                     /locus_tag="PN977_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HDI6041491.1"
                     /translation="MKPIFSRGPSLQIRLILAVLVALGIIIADSRLGTFSQIRTYMDT
                     AVSPFYFVSNAPRELLDGVSQTLASRDQLELENRALRQELLLKNSELLMLGQYKQENA
                     RLRELLGSPLRQDEQKMVTQVISTVNDPYSDQVVIDKGSVNGVYEGQPVISDKGVVGQ
                     VVAVAKLTSRVLLICDATHALPIQVLRNDIRVIAAGNGCTDDLQLEHLPANTDIRVGD
                     VLVTSGLGGRFPEGYPVAVVSSVKLDTQRAYTVIQARPTAGLQRLRYLLLLWGADRNG
                     ANPMTPEEVHRVANERLMQMMPQVLPSPDAMGPKLPEPATGIAQPTPQQPATGNAATA
                     PAAPTQPAANRSPQRATPPQSGAQPPARAPGGQ"
     gene            25243..25731
                     /gene="mreD"
                     /locus_tag="PN977_001444"
     CDS             25243..25731
                     /gene="mreD"
                     /locus_tag="PN977_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001487551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HDI6041492.1"
                     /translation="MASYRSQGRWVIWLSFLIALLLQIMPWPDNLIVFRPNWVLLILL
                     YWILALPHRVNVGTGFVMGAILDLISGSTLGVRVLAMSIIAYLVALKYQLFRNLALWQ
                     QALVVMLLSLVVDIIVFWAEFLVINVSFRPEVFWSSVVNGVLWPWIFLLMRKVRQQFA
                     VQ"
     gene            25740..26333
                     /gene="yhdE"
                     /locus_tag="PN977_001445"
     CDS             25740..26333
                     /gene="yhdE"
                     /locus_tag="PN977_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase YhdE"
                     /protein_id="HDI6041493.1"
                     /translation="MTSLYLASGSPRRQELLAQLGVTFERIVTGIEEQRQPQESAQQY
                     VVRLAREKAQAGVAQTAQDLPVLGADTIVILNGEVLEKPRDAEHAVQMLRKLSGQTHQ
                     VMTAVALADSQHILDCLVVTDVTFRTLTDEDIAGYVASGEPLDKAGAYGIQGLGGCFV
                     RKINGSYHAVVGLPLVETYELLSNFNALREKRDKHDG"
     gene            26323..27792
                     /gene="rng"
                     /locus_tag="PN977_001446"
     CDS             26323..27792
                     /gene="rng"
                     /locus_tag="PN977_001446"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease G"
                     /protein_id="HDI6041494.1"
                     /translation="MTAELLVNVTPSETRVAYIDGGILQEIHIEREARRGIVGNIYKG
                     RVSRVLPGMQAAFVDIGLDKAAFLHASDIMPHTECVAGEEQKQFTVRDISELVRQGQD
                     LMVQVVKDPLGTKGARLTTDITLPSRYLVFMPGASHVGVSQRIESESERERLKKVVAE
                     YCDEQGGFIIRTAAEGVGEAELASDAAYLKRVWTKVMERKKRPQTRYQLYGELALAQR
                     VLRDFADAELDRIRVDSRLTYEALLEFTSEYIPEMTSKLEHYTGRQPIFDLFDVENEI
                     QRALERKVELKSGGYLIIDQTEAMTTVDINTGAFVGHRNLDDTIFNTNIEATQAIARQ
                     LRLRNLGGIIIIDFIDMNNEDHRRRVLHSLEQALSKDRVKTSVNGFSALGLVEMTRKR
                     TRESIEHVLCNECPTCHGRGTVKTVETVCYEIMREIVRVHHAYDSDRFLVYASPAVAE
                     ALKGEESHSLAEVEIFVGKQVKVQIEPLYNQEQFDVVMM"
     gene            27860..31660
                     /gene="yhdP"
                     /locus_tag="PN977_001447"
     CDS             27860..31660
                     /gene="yhdP"
                     /locus_tag="PN977_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA2 domain-containing protein YhdP"
                     /protein_id="HDI6041495.1"
                     /translation="MRRLPGILLLTGAALVVIAALLVSGLRIALPHLDAWRPEILNKI
                     ESATGMPVEASQLSASWQNFGPTLEAHDIRAELKDGGEFSVKRVTLALDVWQSLLHMR
                     WQFRDLTFWHLRFRTNTPITSGGGNDSLEASHISDLFLRQFDHFDLRDSEVSFLTPSG
                     QRAELAIPQLTWLNDPRRHRAEGLVSLSSLTGQHGVMQVRMDLRDDEGLLSNGRVWLQ
                     ADDIDLKPWLGKWMQDNIALETAQFSLEGWMTIDKGDVTGGDVWLKQGGASWLGEKET
                     HTLSVDNLTAHITRENPGWQFSIPDTRITMDGKPWPSGALTLAWIPEQDVGGKDNKRS
                     DELRIRASNLELAGLEGVRPLAAKLSPALGDVWRSTQPSGKINTLALDIPLQAADKTR
                     FQASWSDLAWKQWKLLPGAEHFSGTLSGSVENGLLTASMKQAKMPYETVFRAPLEIAD
                     GQATISWLNNDKGFQLDGRNIDVKAKAVHARGGFRYLQPANDEPWLGILAGISTDDGS
                     QAWRYFPENLMGKDLVDYLSGAIQGGEADNATLVYGGNPQLFPYKHNEGQFEVLVPLR
                     NAKFAFQPDWPALTNLDIELDFINDGLWMKTDGVNLGGVRASNLTAVIPDYSKEKLLI
                     DADIKGPGKAVGPYFDETPLKDSLGATLQELQLDGDVNARLHLDIPLNGELVTAKGEV
                     TLRNNSLFIKPLDSTLKNLSGKFSFINGDLQSEPLTASWFNQPLNVDFSTKEGAKAYQ
                     VAVNLNGNWQPAKTGVLPEAVNEALSGSVAWDGKVGIELPYHAGATYNVELNGDLKNV
                     SSHLPSPLAKPAGEPLPVNVKVDGNLNSFELTGQAGADNHFNSRWLLGQKLTLDRAIW
                     AADSKTLPPLPEQSGVELNMPPMNGAEWLALFQKGAAESVGGAASFPQHITLRTPMLS
                     LGNQQWNNLSIVSQPTANGTQVEAQGREINATLAMRNNAPWLANIKYLYYNPSVPKTH
                     GDSTNSSPFLTTERISFRGWPDAQIRCAECWFWGQKFGRIDSDITISGNTLTLTNGLI
                     DTGFSRLTADGEWVNNPGNERTSLKGKLRGQKIDAAAEFFGVTTPIRQSSFNVDYDLH
                     WRKAPWQPDEATLNGIIHTQLGKGEITEINTGHAGQLLRLLSVDALMRKLRFDFRDTF
                     GEGFYFDSIRSTAWIKDGVMHTDDTLVDGLEADIAMKGSVNLVRRDLNMEAVVAPEIS
                     ATVGVAAAFAVNPIVGAAVFAASKVLGPLWSKVSILRYHISGPLDDPQINEVLRQPRK
                     EKAQ"
     gene            31816..33261
                     /gene="tldD"
                     /locus_tag="PN977_001448"
     CDS             31816..33261
                     /gene="tldD"
                     /locus_tag="PN977_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HDI6041496.1"
                     /translation="MSLNLVSEQLLAANGLKHQDLFAILGQLAERRLDYGDLYFQSSY
                     HESWVLEDRIIKDGSYNIDQGVGVRAISGEKTGFAYADQISLLALEQSAQAARTIVRD
                     SGDGKVQTLGAVEHSPLYTSVDPLQSMSREEKLDILRRVDKVAREADKRVQEVTASLS
                     GVYELILVAATDGTLAADVRPLVRLSVSVLVEEDGKRERGASGGGGRFGYEFFLADLD
                     GEVRADAWAKEAVRMALVNLSAVAAPAGTMPVVLGAGWPGVLLHEAVGHGLEGDFNRR
                     GTSVFSGQVGELVASELCTVVDDGTMVDRRGSVAIDDEGTPGQYNVLIENGILKGYMQ
                     DKLNARLMGMTPTGNGRRESYAHLPMPRMTNTYMLPGKSTPQEIIESVEYGIYAPNFG
                     GGQVDITSGKFVFSTSEAYLIENGKVTKPVKGATLIGSGIETMQQISMVGNDLKLDNG
                     VGVCGKEGQSLPVGVGQPTLKVDNLTVGGTA"
     gene            complement(33389..34318)
                     /gene="aaeR"
                     /locus_tag="PN977_001449"
     CDS             complement(33389..34318)
                     /gene="aaeR"
                     /locus_tag="PN977_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AaeR"
                     /protein_id="HDI6041497.1"
                     /translation="MERLKRMSVFAKVVEFGSFTAAARQLQMSVSSISQTVSKLEDEL
                     QVKLLNRSTRSIGLTEAGRIYYQGCRRMLHEVQDVHEQLYAFNNTPIGTLRIGCSSTM
                     AQNVLAGLTAKMLKEYPGLSVNLVTGIPAPDLIADGLDVVIRVGALQDSSLFSRRLGA
                     MPMVVCAAKSYLTQYGIPEKPADLSSHSWLEYSVRPDNEFELIAPEGISTRLIPQGRF
                     VTNDPMTLVRWLTAGAGIAYVPLMWVINEINRGELEILLPRYQSDPRPVYALYTEKDK
                     LPLKVQVVINSLTDYFVEVGKLFQEMHGRGKEK"
     gene            34501..34704
                     /locus_tag="PN977_001450"
     CDS             34501..34704
                     /locus_tag="PN977_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AaeX family protein"
                     /protein_id="HDI6041498.1"
                     /translation="MSLFPVIVVFGLSFPPIFFELLLSLAIFWLVRRVLVPTGIYDFV
                     WHPALFNTALYCCLFYLISRLFV"
     gene            34712..35644
                     /gene="aaeA"
                     /locus_tag="PN977_001451"
     CDS             34712..35644
                     /gene="aaeA"
                     /locus_tag="PN977_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeA"
                     /protein_id="HDI6041499.1"
                     /translation="MKTLIRKFSRTAITVVLVILAFIAIFNAWVYYTESPWTRDARFS
                     ADVVAIAPDVSGLITQVNVHDNQLVKKGQVLFTIDQPRYQKALEEAQADVAYYQVLAQ
                     EKRQEAGRRNRLGVQAMSREEIDQANNVLQTVLHQLAKAQATRDLAKLDLERTVIRAP
                     ADGWVTNLNVYTGEFITRGSTAVALVKQNSFYVLAYMEETKLEGVRPGYRAEITPLGS
                     NKVLKGTVDSVAAGVTNASSTRDDKGMATIDSNLEWVRLAQRVPVRIRLDNQQENIWP
                     AGTTATVVVTGKQDRDESQDSFFRKMAHRLREFG"
     gene            35650..37617
                     /gene="aaeB"
                     /locus_tag="PN977_001452"
     CDS             35650..37617
                     /gene="aaeB"
                     /locus_tag="PN977_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeB"
                     /protein_id="HDI6041500.1"
                     /translation="MGIFSIANQHIRFAVKLATAIVLALFVGFHFQLETPHWAVLTAA
                     IVAAGPAFAAGGEPYSGAIRYRGFLRIIGTFIGCIAGLVIIIAMIRAPLLMILVCCIW
                     AGFCTWISSLVRIENSYAWGLAGYTALIIVITIQPEPLLTPQFAVERCSEIVIGIVCA
                     IMADLLFSPRSIKQEVDRELESLLVAQYQLMQLCIKHGDGEVVDKAWGDLVRRTTALQ
                     GMRSNLNMESSRWARANRRLKAINTLSLTLITQSCETYLIQNTRPELITDTFREFFDT
                     PVETAQDVHKQLKRLRRVIAWTGERETPVTIYSWVAAATRYQLLKRGVISNTKINATE
                     EEILQGEPEVKVESAERHHAMVNFWRTTLSCILGTLFWLWTGWTSGSGAMVMIAVVTS
                     LAMRLPNPRMVAIDFIYGTLAALPLGLLYFLVIIPNTQQSMLLLCISLAVLGFFLGIE
                     VQKRRLGSMGALASTINIIVLDNPMTFHFSQFLDSALGQIVGCVLAFTVILLVRDKSR
                     DRTGRVLLNQFVSAAVSAMTTNVARRKENHLPALYQQLFLLMNKFPGDLPKFRLALTM
                     IIAHQRLRDAPIPVNEDLSAFHRQMRRTADHVISARSDDKRRRYFGQLLEELEIYQEK
                     LRIWQAPPQVTEPVHRLTGMLHKYQHALTDS"
     gene            37709..37981
                     /locus_tag="PN977_001453"
     CDS             37709..37981
                     /locus_tag="PN977_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="barstar family protein"
                     /protein_id="HDI6041501.1"
                     /translation="MNIYTFDFDEIESQEDFYRDFSQAFGLAKDKVRDLDSLWDVLMN
                     DVLPLPLEIEFVHLGEKTRRRFGALILLFDEAEEELEGHLRFNVRH"
     gene            complement(38037..38300)
                     /gene="yhcN"
                     /locus_tag="PN977_001454"
     CDS             complement(38037..38300)
                     /gene="yhcN"
                     /locus_tag="PN977_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid stress response protein YhcN"
                     /protein_id="HDI6041502.1"
                     /translation="MKIKTTVAALSVLSVLSFGAFAADSIDAAQAQNREAIGTVSVSG
                     VASSPMDMREMLNKKAEEKGATAYQITEARSGDTWHATAELYK"
     gene            complement(38665..39135)
                     /gene="argR"
                     /locus_tag="PN977_001455"
     CDS             complement(38665..39135)
                     /gene="argR"
                     /locus_tag="PN977_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001257852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ArgR"
                     /protein_id="HDI6041503.1"
                     /translation="MRSSAKQEELVKAFKALLKEEKFSSQGEIVAALQEQGFDNINQS
                     KVSRMLTKFGAVRTRNAKMEMVYCLPAELGVPTTSSPLKNLVLDIDYNDAVVVIHTSP
                     GAAQLIARLLDSLGKAEGILGTIAGDDTIFTTPANGFTVKDLYEAILELFDQEL"
     gene            39570..40508
                     /gene="mdh"
                     /locus_tag="PN977_001456"
     CDS             39570..40508
                     /gene="mdh"
                     /locus_tag="PN977_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000861594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HDI6041504.1"
                     /translation="MKVAVLGAAGGIGQALALLLKTQLPSGSELSLYDIAPVTPGVAV
                     DLSHIPTAVKIKGFSGEDATPALEGADVVLISAGVARKPGMDRSDLFNVNAGIVKNLV
                     QQVAKTCPKACIGIITNPVNTTVAIAAEVLKKAGVYDKNKLFGVTTLDIIRSNTFVAE
                     LKGKQPGEVEVPVIGGHSGVTILPLLSQVPGVSFTEQEVADLTKRIQNAGTEVVEAKA
                     GGGSATLSMGQAAARFGLSLVRALQGEQGVVECAYVEGDGQYARFFSQPLLLGKNGVE
                     ERKSIGTLSAFEKNALEGMLDTLKKDIALGEEFVNK"
     gene            complement(40571..41638)
                     /gene="degS"
                     /locus_tag="PN977_001457"
     CDS             complement(40571..41638)
                     /gene="degS"
                     /locus_tag="PN977_001457"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane-stress sensor serine
                     endopeptidase DegS"
                     /protein_id="HDI6041505.1"
                     /translation="MFVKLLRSVAIGLIVGAILLVAMPSLRSLNPLSTPQFDSTDETP
                     ASYNLAVRRAAPAVVNVYNRGLNTNSHNQLEIRTLGSGVIMDQRGYIITNKHVINDAD
                     QIIVALQDGRVFEALLVGSDSLTDLAVLKINATGGLPTIPINARRVPHIGDVVLAIGN
                     PYNLGQTITQGIISATGRIGLNPTGRQNFLQTDASINHGNSGGALVNSLGELMGINTL
                     SFDKSNDGETPEGIGFAIPFQLATKIMDKLIRDGRVIRGYIGIGGREIAPLHAQGGGI
                     DQLQGIVVNEVSPDGPAANAGIQVNDLIISVDNKPAISALETMDQVAEIRPGSVIPVV
                     VMRDDKQLTLQVTIQEYPATN"
     gene            complement(41728..>42606)
                     /locus_tag="PN977_001458"
     CDS             complement(41728..>42606)
                     /locus_tag="PN977_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HDI6041506.1"
                     /translation="VGDFAVAVGNPFGLGQTATSGIVSALGRSGLNLEGLENFIQTDA
                     SINRGNSGGALLNLNGELIGINTAILAPGGGSVGIGFAIPSNMARTLAQQLIDFGEIK
                     RGLLGIKGTEMSADIAKAFNLDVQRGAFVSEVLPGSGSAKAGIKAGDIITSLNGKPLN
                     SFAELRSRIATTEPGTKVKLGLLRNGKPLEVEVTLDTSTSSSASAEMITPALEGATLS
                     DGQLKDGGKGIKIDEVVKGSPAAQAGLQKDDVIIGVNRDRVNSIAEMRKVLAAKPAII
                     ALQIVRGNESIYLLMR"
BASE COUNT        10542 a        10687 c        10976 g        10401 t
ORIGIN      
        1 gcggcagtag cgcggtggtc ccacctgacc ccatgccgaa ctcagaagtg aaacgccgta
       61 gcgccgatgg tagtgtgggg tctccccatg cgagagtagg gaactgccag acatcaaatc
      121 aagcgaaagg ccatccaaaa ggatggcctt tttgcgtttc gaatctatgt acaacttaat
      181 ggataacctg cgataaaaat gccctcgtac gctccgattt aggatgcgca aaaaattcat
      241 caggtgtagc ttgttccact atctccccac gatccataaa aatcacccgg tcagcgacgg
      301 ttcgtgcaaa ccccatctcg tgcgttacgc acaacattgt catacctgac tgcgccagcc
      361 caatcattgt atccagcact tctttcacca tctccggatc gagcgcagac gtaggttcgt
      421 caaacagcat gatctttggt ttcatacaaa gtgaacgtgc tatggcaacg cgttgctgct
      481 gaccacctga aatctgtccg ggaaacttat gcgcatgttc ggcaattctc acccgctcaa
      541 ggtaatgcat cgccagagct tcagcctctt tctttggcat cttgcgtacc caaatcggtg
      601 ccagggtaca gttctgtaga acggtcagat gagggaagag attgaaatgc tgaaagacca
      661 ttcccacttc ctgcctgacg cgctcaatat tgcgaatatc ttcgttaagc tcgatgccat
      721 ctaccacgat ctgtccctgt tggtgttctt ccagatgatt aatacaacga atggttgtcg
      781 atttaccgga accagaaggg ccacacagaa cgatccgttc tcccggttgc acggttaaat
      841 ttatattttt caaaacatgg aattgcccat accatttatt gacgttttcc agcgtaatca
      901 tcgcgttagc aggttgcagt aaaatttggc tcatagtgtc ctcaatgcgg tgtacgcccg
      961 gtgttaaaac gtttttccag atgctggcta tagcgcgaca tgctgaaaca gaagatccag
     1021 tagatcagtg cggcgaaaac atacccttcc gtcgacatac ccaaccaggc gggatcaacg
     1081 gttgcctgct gaacgctact gaaaagatcg aacaacccga tgatgatcac caggctggta
     1141 tctttgaaga gtgcgatgat ggtatttacc agcccaggaa tcaccagctt caacgcctgt
     1201 ggcagaataa ccagcccctg ggttttccag taacccaacg ccagcgactc tgccgcttca
     1261 tattgccctt taggcagcgc ctgtaatccg cctcgcacga cttccgcaac atatgctgac
     1321 tgaaacagga tcacgccaac cagcgcgcgg atcaatttgt cgatactggt gccttctgcc
     1381 ataaacaacg gcagcatgac cgaagacata aacagaacgg taatcagcgg tacaccgcgc
     1441 cagaactcga taaaaatgac cgataagata cgcacaatcg gcatatggga gcgacgacct
     1501 aacgccagta agatccccca cggcagcgcc cccgcaatcc caactgatgc aataattaaa
     1561 gttagcgtca gcccgcccca ttgccgggtt tcaacccgct caagaccaag aaacccgcca
     1621 tacatcagcc accagacaat cagtgggtaa atcaccgccc ataccgcaat atagcgaccg
     1681 cgatacggga gtattttcca gaacattggt gcgatcgata caagcccaat cagtaatgcc
     1741 aggttaattc gccagcgttg gtcgtgtggg taaagcccat acataaactg accaaatcgt
     1801 tcgtggatga agacccaaca ggcaccggct tttgtgcagt cggcacgcgt tgagccaacc
     1861 cagttagctt gcaaaaatgc ccagttcagc aacggtggga tcagttccca catcaaccat
     1921 atgcagccaa tagtcagcag gctattgctc aagctggaga acagattttt tcgcacccac
     1981 accatcgcgc ggcttgagtt atggctcgcc gggcgcgggg gatgagacag caatactttt
     2041 gtcatcatga ctccttagcg ttcaacgatc gcgatgcggc ggttatagat attcatcagc
     2101 agcgagatag tcaggctgat aatcagatag accgacatgg tcatggctat cgtctcgatg
     2161 gcttgccccg tttggttcag cacggtgcca gcaaacagcg aaaccatatc gggatagcca
     2221 atagcggcgg caagagagga gttcttgacg atgttgagat actggctggt taacggtgga
     2281 ataatcaccc gcaatgcctg gggaataatg acctggcgta gcgtgaccgg attgggtaat
     2341 cccagtgacc gagctgcttc atgttgacca taaggcactg cctggatccc tgcgcggata
     2401 atttcggcga taaatgcaga agtataaacc gaaagcgcca gcgttaaggc tgccagttca
     2461 ggaattaaaa ccatcccgcc gcggaaatta aagcctcgaa gggctgggac atcccagtgt
     2521 agtgctgcgc caaaaagcca ttgcgccagt aaaggcaaac caatgatcaa cactgcggcg
     2581 atcggccagg tgcggcgcag ttgtccggtt tttatctggt atgttttatt aaagcggaat
     2641 agcccaacag aaaggactat agccataaca acagccagga taaacgcaat aaatccgtct
     2701 cccagctgcg gcgacggaat ataaagccca cgattgctca aaaaggccag atcaaacgca
     2761 ctcactgcct ggcgtggtcc gggcaaattg cgcagcacgg caaagtacca gaagaagatt
     2821 tgcagcagcg gcggaatatt acggaagatc tcaatataaa ttgtggaaag ctttcgtagc
     2881 agccagttat ccgaaagtcg cgccagaccg ataaagaagc ccagaacaga agcgaataca
     2941 atacacaatg cagaaaccag tagcgtattg agtaagccaa caatgaaaac gcgtccgtag
     3001 gtatcaccct gctggtaatc gatcaaatgc tggacaatac cgaagccagc gccgcgatcc
     3061 agaaaggcaa aacctgaagt aatgccacga ttactgagat tcgtcacagt gttgtgaaat
     3121 agccaaccaa caatgccaac aacagcaaca acggcaagga tctggaataa ccaggcgcga
     3181 accgtagggt tggcaaagga gagtgagcct ttaacggttg agcggcgatg agacatagaa
     3241 acctcggaaa gcataacgct gaagagagcg ccgccgtagc gacgctcgtt acctcacacc
     3301 gacttaacgc acgggcggcg cgtactgaat tccgccgtta ttccagagat tattttgccc
     3361 acgtttaatt ttcagcgggc tttctgaacc tacgttacgc tcaaaaattt ccgagtagtt
     3421 acccacctgt ttgatgatgt tgtaagccca tttattatcc agcttcagat ccttgccgta
     3481 atcgccttct ttacccagca gatgtgccat atcaggcgtt gccggattag ccgctttttc
     3541 atcgacgttc tgggaattga tgcccatctc ttcagcattc agcatggcga aaagcgtcca
     3601 gcgtacaatc gagaaccatt catcatcgcc acggcgaact accggcccaa gcggttcttt
     3661 ggagataact tccggtaaga caatccattc agccgggttg cttaatttga tgcgcagggc
     3721 atacagttgt gattgatccg aggccagcgt atcgcagcga ccagattcca gtgccttcgc
     3781 tgattcgtca gagcgatcga aagtcactgg tgtgtacttc atattgtttg ccttaaagta
     3841 atcggcaacg ttgagttcgg tatcagtacc cgcctgaata cagacggtag cgccatccag
     3901 ttctttcgcg ctttttagtc ccgctttatc gtgagtcagg aagccaatgc cgtcgtaata
     3961 agtgacgccg gtaaatgcca ttcccatccc ggcatcgcga gatgaagtcc aggtcgtatt
     4021 acgggagagc aaatccacct cccctgactg taaagcggtg aagcgttctt ttgcagtgag
     4081 cggggtatat ttcactttcg tgtcgtcacc aaatacagca gcggcaacac cacgacaaat
     4141 atcaacatca atacctgaaa atttaccgtc agcatcggca taagagaacc caggtaatcc
     4201 atcactgatc ccgcattgca caaaaccttt tttctgtacg gcatcaagcg tcgcgccagc
     4261 atgcgcctga tttgcaacgg caagcagcac gctggcggca gccagtgtgg ctatcatcat
     4321 ctttttcata atgcttcctg tgaggcgaaa attatcgtta taggacattt gggaaacgtc
     4381 ttttacctgt ctgtggccat cctcttataa gcaaaagtag tgccagtttt tgcgcacgat
     4441 ttcgcgtcgc aataagctga gtgtagacag gaaataataa aaacagcgcc ccgcaatggt
     4501 gcgaaaatga aacatgcgcc acattttggc gcaaaatatt tacgcgtttt ttactaagca
     4561 gaataatgat tgttagcgtg agtataattc tggtgggaag tgtgaaaatt aaattgatgc
     4621 gttatttcga aagcagcgaa ataacgcgat ataacatgct aaatcagcag ggaattagcc
     4681 agcagaatat agcaatattt tatcttatgg tgagatactg tatcgttaaa cagagcgcgg
     4741 tactccgcgc tctgttcagt tatttcgaca gagcgatgat ccccatcgtc aggccagcaa
     4801 cagctgctgc cccgccagca atagccccag ccgtttccca gttatcctga gtcgtgcctt
     4861 tcatacaggt attggtgtga accagtcgcc cctgatcatc ataaaccgga acgcagggcg
     4921 aatcgtgagc acagcccgcc agcaatgcgg tgagcaatgc cacaggaatt aatcttttca
     4981 tggtgttacc tcagtaataa acgctaacgc ttaataagtt aactctctta ttaacgagct
     5041 gaatgccatt ttatcaccgg aggttataaa tcttgcggaa tgaataatga caaattatgt
     5101 gaattgtaaa aatagtgaag ggagcgggaa ctatagaaag tttttaaaga gtgagaagaa
     5161 gaatatctta ttaattttta tgggtttcac tgaaaataat aaaggcaccc gaaagtgcct
     5221 ttatgtttct gatttatcct ttaaagcaac ggcggatcac cacaaagaac accggcacga
     5281 agaagattgc cagcaacgtt gcagaaacca tcccgcccat taccccgata cccactgcgt
     5341 tctgcgcgcc actgccggca ccgttactga tagctagcgg taatacgccg agaataaagg
     5401 cgagagaggt catcaggata ggacgcagac gcatacgtac tgccattagt gtcgcttcaa
     5461 caacaccttt accctctttt tccatgagat ctttagcgaa ctcaacaatc aaaatagcgt
     5521 ttttggccga caagccaatt gtcgtcagca agcccaccat aaagtagacg tcattttttt
     5581 gattaaagag tgttgccgcc agtagcacgc cgacaatccc taacggcacg accaacataa
     5641 ccgagacagg aattgaccag ctttcataga gtgcagcaag gcacaggaaa acaaccacaa
     5701 aggaaattgc taccagagcg ggagcctggt ttcccgataa acgttcctga tatgacatac
     5761 ccgtccagtc ataaccaatg cccgccggta attttgacgc aaggttttcc atcaacgcca
     5821 tggcatcgcc ggaactggtt cctggcgcgg cttccccctg aatctccatt gacggcagac
     5881 cgttgtagcg ctccagtcgc ggagagccat acacccaatg tgcagtggta aaggcagaga
     5941 atggcaccat ttcgccgttg gcgctgcgga cataaagttt atcgacatct tctggcagca
     6001 tacggaattt ggcatccgcc tgaacataca cctttttcac gcgaccacgg tcaatgaagt
     6061 cgttaacgta agtcccaccc aacgccgttg aaatggtctg attgatgtca gaaagtgaaa
     6121 cacctaatgc ctgcgccttt tcctggtcaa cttccagttt aaactgcgcg gtgtcttcca
     6181 gaccattagg acgcacgctg actaagctgg caggatgttg cgccgccatg ccaagcaact
     6241 ggttacgggc ctgggtcagg gcatcgtgac ccagcccagc ctgatcaatt aactcaaagt
     6301 cgaaacccgt tgccgtgccc agttcaacaa tggctggcat attgaatgga atgacaaaac
     6361 cgtcgcggat cttgcccaat tccattttgg cacgatggat taccgcttcc gcactgtttt
     6421 cgtcaccact acgctcttcc cacggtttca gactgacgaa ggccataccg gcgttttgtg
     6481 cctggccgct gaagctaaag ccgttaaccg taaagacact ttctacgttc gctttctcgt
     6541 tcttcagata gtaatccgtc acttgatcca acactttttg cgtccgctct tgcgtcgcgc
     6601 cagcgggtaa ctgaatcata gtcagaaaga ctccctgatc ctcttcaggt aagaaggaag
     6661 acgtaaaaac aacaccacca ttccagcaac aattagtgca tagatcagta aatatcgtcc
     6721 tgtggaaccg aggattttgc cgacgctgtt ggtgtagtgg ttaacgctat gatcgaaggt
     6781 ggtattaaac cagccgaaga aaccgccctt attttcgtga tgctcagcag agacgggttt
     6841 gagcagtgtt gcacataacg ctggggtaag aatcaatgcc accagaacag aaagcgccat
     6901 tgccgaaacg atggtgatag agaactggcg gtaaattgcc ccagtagaac cgccgaagaa
     6961 tgccattgga ataaataccg ctgacagcac catcgcgata cccaccagtg cgccctgaat
     7021 ttgcgacatc gatttttccg tcgcttcttt tggcgggagc ttatcctcca tcatcacgcg
     7081 ctcgacgttc tccaccacca ctatcgcatc atcgacgagc agcccgatgg caagcaccat
     7141 cccgaacatc gttagtgtgt tgatggagta accaaaagcg gcgaggatgg caaacgtccc
     7201 taacaacacc acgggtaccg caatggtggg gatcagcgtt gctcgcatat tctgcaagaa
     7261 cagatacatc accaggaaca ccagcataat ggcttcgaac agcgttttta ccacttcgtg
     7321 aatagaaagc tggacgaatg gtgtggtgtc ataagggtag agaaccttca ttccctgcgg
     7381 gaagaatggc tgtaattccg ccagttttgc cttaatggct ttcgcggtat cgagagcatt
     7441 cgcgccggtt gccagcttaa tacccaggcc cgccgccggt ttaccgttga tgcgagcgat
     7501 aacgttatag ttttcaccgc caagttcaac ccgtgcgaca tctttcaggc gtaccaccga
     7561 gccgtcactg tttacgcgca aggtcacttt gccgaattct tccggatttt ttaaccgcgt
     7621 ctgagcaata atcgaggcgt tcaactgttg ccctggtaac gctggcgttc cgcccaactg
     7681 tccggcagcg atctgatcgt tctgtacctt caactggtta atcacatcaa ccggcgtcag
     7741 tttgtatttg tttagcaggt cggcgtccaa ccagatacgc atcgcatact gtgcgccgaa
     7801 aagttgtacg tcaccgacgc cattcagacg gctaagcgta tctttaacgt tagaggccac
     7861 atagtccgag atatcgtcct gtgtggtgcc tgggttatca gagacaaagc ccgccaccat
     7921 caaatagctg ctactggact tttcaacact gatcccctgc tgctgtacct cctgcggcag
     7981 caacggcgtg gcgagctgga gtttgttctg cacctgcact tgcgcgatat ctggatcggt
     8041 cccggactgg aaggtaaggg taattgtcac gctaccggca gaatcgctgg tggaggacat
     8101 atacatcagg ttatcgatac cattcatatt ctgttcgata acctgcgtca ccgtatcctg
     8161 cacggtctgc gcatcagcgc ccggatagtt tgccgaaaca gaaactgcag gcggcgcaat
     8221 ggttgggtat tgggcgacgg gcaattgtag gatcgccagt acacccgcca tcatcagaat
     8281 aatggccagc acccatgcaa atatcggtcg tcgaataaaa aagtttgcca tgtcagattg
     8341 ccttacttcg atgcagaatc tgcgggggta tcggtagtgg ctttaacctg ctctcccgga
     8401 cgcgcttttt gcaggccgct gacaatgact tgatcgccag atttcagccc ttcgctaatc
     8461 aaccatttat cgccaatcgc ctggctggca acgacagggc gcgcttcaac ctgactttta
     8521 tcgttaacaa tcagcacggt tgcatcacca cgcggtgtgc ggctaacgcc ttgttgcggg
     8581 ataagaatgg catcagattg gacgccttca tcaatccgtg cgcgcacaaa cataccaggt
     8641 aaaagcgtat gttgcgggtt agggaagacg gcacgtaggg ttatggagcc ggtgctttca
     8701 tcaacggtaa catcggagaa ttgcagcgta cctttcaggg gataggtttg accgttttcc
     8761 atgaccaact ctacgttgct ggtggcgttt tccttatgca aatttccttg ctctacggat
     8821 tgcttcagcc tcataaaatc gttgctggat tgggtcacat caacgtagat aggatcgagc
     8881 tgctggacag tcgccagttc agtcgtttgc ccattagtga caagagcgcc ttcggtcacg
     8941 gtcgatttgc cgatacgtcc gctaattggc gcagtcactt tggtataagc gagattgatg
     9001 cgagcgcttt cgactgtggc ttttgcggca atcacggcgg catcggcctg gcgagcatca
     9061 gcaatggcct ggtcgtactc ctgctgactg atgtatttcg tacccacgag cggaacgtaa
     9121 cgttttaccg tcaaatgcgc gatggcggcg gcggcttcac ttttcgccag ttcgcctttc
     9181 gcgctgtcat aacttgcctg ataggtcgcg ggatcgatct ggtacagaga ctggcctgct
     9241 tgcacatcgc tgccttcagt gaaattgcga ctcagtacga tcccgctaac ctgtgggcga
     9301 acttcggcta tacggtaagc attggtgcgg cctggtaatt cagtcttaac ttctaacggg
     9361 gccgttttca caatatgaac ggtaacctgc ggttcaccga cgtgagcttt ctcttctccc
     9421 ttatcgttac aaccggcgat taaagccgcg gagatcagaa taaaggaggg caggaggaaa
     9481 aacctggcat gtttcgtcat tactattcct caaaaaacca aaagcgcgtt atttacccaa
     9541 aaaggcaacg cgttaactcg cagaaagaaa aatacagttc gctatcctac aaattatcat
     9601 tcgtcgatgt aaggaatagt tatgaatact ggcgtctcaa ggcacataaa cacaaaaaaa
     9661 gattaatatt ctactgtttt attttgacgc gggttgaaag aggtagaatc aaatcctcgt
     9721 aaattgaaat atatattgat gtagtgaatg tatcttaggt aaataatata tattatttat
     9781 ttaacgattt atagcaactc aatattagcc tctcgtataa atacacatta ggtgatagat
     9841 taaccttcgc tattttctca ctctgtgtcg aatatattta tttcctgaat aattaatcat
     9901 ggcaaaaaga accaaagccg aagctctgaa gacccggcaa gaactgattg aaactgccat
     9961 cgcccagttt gcgcaacatg gcgtaagcaa gacgacgctc aacgacattg ccgatgccgc
    10021 taacgttacg cgtggcgcta tctactggca cttcgagaac aagacccaac tgtttaatga
    10081 gatgtggttg caacagcctt cattgcggga gttaatccag gaacacttga cggctgggtt
    10141 agagcatgac ccgtttcaac aattgcgtga aaaattgatt gtcggcttgc aatatattgc
    10201 caaaattccc cgccagcagg cgttgctgaa aatcttatat cacaaatgtg aatttaatga
    10261 tgagatgctg gcagagggag tgatacgcga taagatgggc tttaatccgc agactctcca
    10321 cgaagtattg caggcatgtc agcaacaagg ttgtatagca aataatctcg atttagatgt
    10381 tgtgatgatt attattgatg gtgccttcag cggaattgtt caaaactggt taatgaatat
    10441 ggcgggttat gatctttata aacaagcacc cgcactggtc gataacgtat taagaatgtt
    10501 catgccagat gaaaacataa cgaaattaat tcatcaaacg aatgaattaa gtgtcatata
    10561 aattcacatt ctgtcctgaa aattcagttc gtcacagtta accagtttta gtggattaac
    10621 tgagttcata ttgcggcatt tgtctgtttc tgttgccagc agttcaatcc actgcatcag
    10681 taaagtatca aacagaaaaa cgaagacagc gaaaacgacg agccaccaat acttgcgaat
    10741 cattacctga gcctgtgcat gtttaaccaa gtctggcagg aaatatacac gaaatgaata
    10801 atctgaaaac caggcttaca cttcctttac tttgcaatga gatcggggtc aacttcgctt
    10861 aacctgcttc gttttgacag gttgccggtc tttctttttt tcactttcgc cagttcttcc
    10921 gcagagtaat gcgacgcgac atccagccgt cgaagcccca ttttgatgta ctcgctgttg
    10981 atctcaatac caatgaattt tcgcccgctg gcggcggcta cggcaccggt agtaaagcta
    11041 ccggcaaacg ggtcgagaac gatatcgcct gggttggaag aggcgagaat aatgcgtttc
    11101 agtaaggctt ccggtttttg cgtcgggtga ttctcatatt catccatcaa ataacgcacg
    11161 cgcggaaaat cccagacgtt acccggtact ttttgatgat tgtaaggctg tggaggattt
    11221 ttgcgataat cgatcaacgc gcgctgcgat ccggttttgg cttctaccag aatagcatca
    11281 ccgttgaacg tgtagttctt tgcgtctttc accatcatca ggatgggttc gtacatggag
    11341 ccgtagtgtt ttttcgcctg cactccagaa ctgtcatatg accagacgat gcgacttttg
    11401 atggtaaaaa gcttgcggca ctggagatcg ataaagggca tgttttccgt gctgttcatg
    11461 atgtacatgc tgccctgctt tttcagaacg cggtggcact ctgcaatcac ttcaaacagc
    11521 cagtcgataa acagatcttc tttccaggct tcgatcagac catcaaaatt tttaccgatg
    11581 ttatacggtg ggtcggcaaa gatcagatct acactatcgg cggggatctt tttaagttcg
    11641 gttaatgcat caccctggat gatggtcttt gcttcattac caaatcgggt cggttcacat
    11701 cctgttctca tggtcactcc ctttgtgaca cctataaaaa aggcgcttcc ccatgccgag
    11761 tagcgccttt ttaatcaagc atttagctaa cctgaattag ttcatgccgt attttttcaa
    11821 ttttttacgc agcgtaccac ggttgatgcc catcatcagc gcagcacggg tctggttacc
    11881 acgggtgtat tgcatcacca tgtccaacag gggctgttct acttcagcca gtaccagctc
    11941 atagaggtca ttcacatcct gaccgttcag ttgagcaaaa tagttcttca gtgcctgttt
    12001 aaccgagtca cgcaggggtt tttgggttac ctgatcctga gagttaacgg tagaaacggt
    12061 cagtacgtca gaatttacgc gttgttcgaa catagttctg tcagctcttt atttctgttt
    12121 acgcaaaatt ttcgaagtat gcctccaacg cctccagctg ttcgctggca tcctcaatgg
    12181 cgttgaatgt gcgccgaaac tggtcatttg gagcgtgttc ctggagatac caggaaacgt
    12241 gtttacgtgc aattcggtac ccttttgccg gaccataaaa gtcatgcagt tcccgaacgt
    12301 gcgcgcaaag caagcgctta acctctgcca aaggcagcgg gggcagcaac tccccagtgt
    12361 ccagataatg ctggatttcc cgaaagatcc agggtcttcc ctgagctgcg cggcctatca
    12421 tcagggcatc ggcccctgta tagtcgagca cagctctggc tttaagcggg tcagtaatgt
    12481 cgccattcgc gataaccgga atggaaactt tctgcttaac tgcccgaata ctgtcgtact
    12541 cagcttctcc attgaacaaa caggcgcgtg tacggccatg aatggtcaga gcctgaatgc
    12601 cacagtcttc agccagttgg gcaatctctt cgcagttacg gtgttccggc gcccagccgg
    12661 tgcgaatctt cagggtaaca ggaacgtcca ctgcattgac gacctcggta aggatcgatt
    12721 taacgacatc cgggtactgc aaaagggctg aacctgcgag cttgcgattc acttttttag
    12781 ccgggcaacc catattgata tcaataatct gggcaccgct ttccacgtta atacgtgctg
    12841 catctgccat ttctttcgga tcgctaccag caatttgcac ggtgcgaata ccgggttcat
    12901 caatgtgcac catccgtaaa cgagatttgt cgctttccca aacctgtggg ttagaagaca
    12961 tcatctcgga tactgtcaat ccggctccca tctcgtagca caacgtccga aaaggtctgt
    13021 ctgtaatgcc agccatgggc gctgcgatca ggcgatttct gagctgatat tgtccgatgc
    13081 gcatgagtta agaaatgacc atactgtgac tgcaaggcgg cgtatattac gcattttttg
    13141 cacgagatga aaggccaaac tttgaccaat cctctgagat ggatcaaaga attgcattta
    13201 aaatgagcgt ggcgcgataa ttactcataa aaatcatcat attagaaaat gttgactaaa
    13261 atttatactc aaagaaattt gagtaagttc tcaatttttc tttatgaatg aaaattttgg
    13321 cacgcaaatt tgcgtaaata atcggcaaat taacgtgcct tttgtgacct tgctcgcact
    13381 tcgccttgcg tcaccatacg gcgatgcgaa ggttaattct tacgaccggt aatgcggcac
    13441 cactcttctt tttccacgac cgggtccaga gcgaagctgt cggcataagc ttcacaaacg
    13501 ctctctgcct ggcttgccag aataccggaa aggcccagca agccacctga aaccggcagg
    13561 acgctgatta acggtgccag ttcacgtaac gggcctgcga ggatgttagc gaccaccacg
    13621 tcggctttca tcgcttctgg ctgatctttc ggtaagtaga gttccagacg gtcagaaacg
    13681 ccattacgtt cggcgttgtc gcggctggcc tgaatcgcct gcggatcaat atcaatacca
    13741 atggcttttg ctgcacccag tttcagcgcc gcgatagcca gaatgccgga accacagcca
    13801 aagtcgatga ccgttttacc ggttaaatcg aggctgtcga gccattgcag gcacagagag
    13861 gtagttggat gagtccctgt accaaacgcc agacctgggt ctaacatcac gttgacggcg
    13921 ttttcgtccg gcacatcacg ccagcttggg cagatccata gtcgttcacc aaagcgcatc
    13981 gggtggaaat tatccatcca ttcgcgctcc cagtctttat cttccagttg ttcgatttta
    14041 tgcgcgaagc ctgcgccgag cagcggatgg ttttccagaa tcgccaccac gtcgttcata
    14101 tcggtttcag cgtcgaacag accaatcaca tcggtgtcgc cccacaggcg cgtttcgccc
    14161 ggcagcggct caaacacagg cgtatcgtgg gtatcctgaa aagtgataga aacggcaccc
    14221 gcttccatca gcgcatcgct aagatcttcc gcgttcgcgc cggtggtgtt cagtttcagt
    14281 tggatccaag gcatggcaaa actctttatt tatcagtagt caaaacggta gcttgcggga
    14341 cggatgtacc gaaacggttt ccgaccatga aagccagcaa acttagtagt aacgagggca
    14401 cgatagggtg gaagcccagg tactgaatat tcagcgtcgc gagtacggca tacagtacgc
    14461 cgccaacaat catcgcactt agcgcgcctt tggcgttggc gcgttcccag taaagaccca
    14521 gtaccagcgg ccacaggaaa acggcttcca gcccaccgaa ggccagcaaa ttcagccaga
    14581 tgatcatttc tggcggcttc caggcggcaa gcagcagcaa cgcaccgaga accaacgtga
    14641 tcaccgccga catccgcttc agacgcgtct cgttttgcat ttgatccgga cggatattca
    14701 gatagagatc tttaatgatc gtagcggaac tttgcagcaa ctgggcattg atcgtcgaca
    14761 tgatcgcagc cataggtgca gccaggaaga tcccggcagc aaacggtggc agcactttta
    14821 ccattaacgt cgggatcacc agatccggta cggtgagatc ggggatcacc gcacggccta
    14881 acgctccggc caggtgcata ccaaacatca ggactgccac tacaattgtc ccgatgatga
    14941 ttccccgatg taccgctttg ctgtctttat aagagatgca gcgcacagcg gtatgcggca
    15001 gaccaatcac accaaagcac accagcaccc agaacgatgt cataaaggca ggcgacagaa
    15061 tatcgtcagc gccttgcgga gtaaccagtt gcggatcgat ggtttgcaac gtctctactg
    15121 cgttgcttaa gccacctgcg gcatgtacca cgccaataag cagcacaatg gtgccaatca
    15181 gcatcaccag cccttgcatg gtgtcgttca gcacgctggc gcgaaagcca ccaaaggcgg
    15241 tatacaacgc aatgctgata ccgaaaatca gcagcccggt ttcataaggg atacccgccg
    15301 cagtttccag cagacgcgca ccgccgataa actgcacggt catcgcacca acgaacgcaa
    15361 ccagcaaact taaactcgcc agccacacca gcagacgact ctggtagcgg gcaaacagca
    15421 tatcgttcag cgtcaccgca ttgtagcggc gcgcaagaat tgcgaacttc ttgccgagaa
    15481 taccgagcga aagccagact gcaggaagct gaatcatcgc cagcaatacc cagcccaggc
    15541 cgtatttata agcagctcca ggcccgccga taaacgaact ggcactgata taggtcgcgg
    15601 tgagtgtcat cgccagcaca ataccgccca tagaacggct gccgaggaaa tactcattga
    15661 ggaaggtgcc ggtgttccgt ttacgcattg cataaactga aatcccgaac accaccacca
    15721 gataggcgac cagcggtaga atcacttcaa gctgcatcgt catcctccag cgggatatcg
    15781 cgatagataa atttcaccat cgcccagcac agtccaataa acagcagcgg cgtcaggatg
    15841 caagccatct caaaccagcg tggaaagccg gtaaaaccgg gggcaacgcc aggtaagtaa
    15901 gcggctacta accataccgc cagatacaaa agggtcagcc ccagcgccca gcgcgcctct
    15961 ttatgggcct gaacaaaacg agtgtccatt ttttgtccct gatgggtgaa gaaagcgggg
    16021 attgtacctt atgggggttg tcgatcccca gtaataaaaa aggccggaaa atccggcctt
    16081 ttgacgcttt tgcagtctta tttttcctga agaccgagtt ttttctccag atagtggatg
    16141 ttagtgccac catgctggaa gttctcgtca ttcatgatgc ggatctgcag atcaacgttg
    16201 gttttgatac cgtcgatgat cagctcctgc agcgcattct tcatgcgggc aatcgccacg
    16261 tcacggtttt caccgtagca aatcagctta ccgatcattg agtcatagta cggcggtacg
    16321 gtgtagcccg cgtagatatg agactcccaa cgtacgccaa aaccgccagg tgcgtggaaa
    16381 cgggtgattt tgcccggact tggcaggaag gtgttcggat cttcggcgtt gatacgacat
    16441 tccaccgcat ggccgcgaac gtgaacttct tcttgcttga tcgacagcgg ttgaccggca
    16501 gcgatacgca gctgttcttt gatcaggtca acgccggtga tcatttcagt aaccgggtgt
    16561 tctacctgaa tacgggtgtt catttcgatg aaatagaact cgccattttc gaacaggaac
    16621 tcgaaagtac ctgcaccgcg atagccgata tcaacacatg ctttagcgca acgttcgccg
    16681 atgtagcgac gcagttccgg ggtaatgccc ggtgctggtg cttcttcgac cactttctgg
    16741 tggcggcgct gcatggagca gtcacgttcc gccagataga tagcgttgcc ctgaccgtca
    16801 gccagtacct gaatctcgac gtggcgagga ttttccaggt atttctccat gtaaaccata
    16861 tcgttgctga aagcagcttt cgcttccgca cgggtcatgg agatggattg tgccagttca
    16921 gcgtcgccgc gcactacgcg cataccgcga ccgccgccgc cgccggaggc tttgataatt
    16981 accggataac caatgcgttt agcaatggcg cggtttttat ccatatcgtc gcccagcggg
    17041 ccgtcagaac ccggtacgca aggaacgccc gcttttttca tcgccgcgat tgcggatact
    17101 ttgtcgccca tcaggcgaat ggtttctgct ttcgggccga tgaagataaa gccggagcgc
    17161 tcaacctgct cggcaaagtt ggcgttctcg gagaggaagc cgtagcccgg atggattgct
    17221 actgcgccgg tgatttcagc ggcgctgatg attgccggga tgttcagata actttttact
    17281 gacggagcag ggccgataca gaccgtttca tctgccagta atacgtgttt tagatcgcga
    17341 tccgcgctgg agtgcacagc gacagtcttg atgcccagtt ctttacaggc acgaagaata
    17401 cgcaatgcaa tctcgccgcg gttggcaata acaattttat ccagcatgtt cgcctcgtta
    17461 ctcgatgacg accagcggct cgtcaaattc taccggttgt ccactttcga ccagaattgc
    17521 tttcacggta ccggatttgt ccgcttcgat ctggttcatc attttcatgg cttcaacgat
    17581 gcacagggta tcgcccacgt tgactttctg acccacttcg atgaacgctt ttgcgtccgg
    17641 gcttggggtg cggtagaaag taccaaccat cggggaacgt acgatgtgac cactgatttc
    17701 cgctgctgct ggcgcttcca tggaaggaac ggtcgccgga gcggctgcgt tagattgagc
    17761 tggctgctgc atcattggtg cagcgtaagc ttgttgcatc acagggaaac ttgcggcagg
    17821 agctgcacgg ctaatgcgta ctgactcttc gccttcagaa atttccagtt cggagatgcc
    17881 tgattcttca accagctcga tcagtttttt aatcttacga atatccatga gtgggttccg
    17941 tactctttgt ttagtgtgat tgtgacaggc gtttcaccgc cgtctgtaaa gcgtatgccc
    18001 cgacagctgt atgcatagcg ataaattcca gcaggccggg acgccggtct attttgcccc
    18061 agagtgtcaa tgttagactt gacggacatt gtgcagcgat ctgctgctat cctccggcaa
    18121 aaaacaaaat ataccttcgt tgcgccaatg tcaccttttc cgcagcgcaa aaactgcgtc
    18181 agggaggacg aggtcgcaca ttataacgat ttcgtagcaa ttggcagcta aatactggtc
    18241 ttatcaggga agataatcaa cagctaacat gtaaataacc ttcaacaccg tgtaatttgc
    18301 aacaagccgc acaattcacg aaattagcgc catcatcgac ggaacttctt ataacgtaag
    18361 gtgaaaagct gtaaagccag ccctgcgaag atgaggggct gcaatgagat aatcttcaca
    18421 gaccacaaat aatgtaggga tgccaggatc gcgacaagat agacgacatt acgcagcggt
    18481 tgctggggat tacccaattt tcactgcatc gtctggatag atgtgaatgc taaagcacgc
    18541 aaaatcaacc gcctgataat gctcagcatg aaataggttt aagtggttaa tttgtaatca
    18601 aataaaatca gattattccc cccattttgc agaaggtaaa ctgttttgtc aggcggcagt
    18661 gcggctatgt gatgcattca gaatttgccg cgtgacaata aaaaagccga tttggtggca
    18721 aaatcggctt cggtgagttt gcagcattca tcacatactc cgacaagctt tttcaagcat
    18781 gacggaggtt gcgttaatgc aaggtttcta cccagggata aaagtgaccg ttacggcgga
    18841 tgaatatctg tgttgtcaga ggatggtcat cctcacggaa gcattgttct gttaactgtc
    18901 gtaatgcgct gagatttgct ggctgatcat tgtaggtgaa aaacagtttt cctctggagg
    18961 gaacagtcac taacaggtcg ccgcattcag gatcgggtga ttgcacccaa agatcgtcaa
    19021 tgagcatcag actgggctca taagtacccc ctgcggagat aacggcaact tttccagcgt
    19081 ttgcgcctgt atcaacgatt tcagccgaaa cttccggcaa tatttgcttc aaattattga
    19141 tggcttgttc gcggtcaatg gtcacttcgt agcgcatcag atagcgcatg gtttcgctac
    19201 catcttcagc aaacaagata attaactcat cgttaaagtc ttcccaggca aaatccagcg
    19261 aggcgttgag gatctctgga tcagattcat actggctaag ctgttggcga acggtatcca
    19321 gccaggcttt ttctttaatc accggaacaa tacagcgccc ggtcggcagc atttctgcaa
    19381 gctgtttcag catactgcag ccacttcttc cagcgggtct ggtgcggctt gataacgttt
    19441 gtaggcatca tcaagagtga tttccaccgt ttggtgaccc gtatcaatgc gcatcaattt
    19501 atggtcagtc gaaatcatga ctttccagtt tggatgatgt tgtacaactt catgaccaag
    19561 atgttcgacg aaagctgtcc ggctaaccgc ttttcccgtt aaccgattga ataatgattt
    19621 caaaatgagt tccctctctt attaatcctt gctaaatggt tagttaacct tcaccagcgt
    19681 gcgcccctgg atctggttat taatgatggc ctcggcaaac ttcggtgcct ctgccagaga
    19741 tatctctttt gccgcctggg tatagaatga ttccggtaaa tcggcgacca atcgctgcca
    19801 ggcttgtgcg cggcgtgctg gtggcgtcat tactgaatcc accccctgta aacggacatt
    19861 acgcagaata aatggcatga ccgtggttgg cagagtaaaa ccacccgcca gaccacaggc
    19921 cgccacgcag ccgccgtaat tcatttgcgc cagcactttt gccagcactt tgtcgccaac
    19981 ggtgtcaatt gccccagccc agacttgttt ttccagagga cgggattcgg caaactcatc
    20041 gcgagggaga atacggctgg cacctaaact tttcagatat tcatgggtac tttcgcgacc
    20101 ggaaacggca acgacctgat aacccaactt atgcagcagc gccacggcgg tactgccgac
    20161 gccaccactg gcacccgtca cgacaatctc cccatcctgc gggcgaacac cggcatcttc
    20221 cagcgccatc acacacaaca tggcggtaaa accggcagta ccgataatca ttgctttacg
    20281 cgcgtccagc ccttgcggca tggcaaccag ccagtcacct ttcactcgcg cctgttccgc
    20341 cagcccaccc cagtggtttt caccaacgcc ccagccagtg agtaacacct cctgaccggc
    20401 atgaaaacgc ggatcttcgc tggtgcgtac agttccggca aaatcgatcc caggaatcat
    20461 cggaaaatta cggatgattt ttcccttacc ggtaattgcc agcgcatctt tgtagttcag
    20521 gctcgaccag tgaacatcaa ccgtgacatc gccctccggc aggcgacttt cgtccagagt
    20581 ctgtaccgat gcgagagttt tgccgtcctg ctgttctaaa agtaacgcct gcataagtgg
    20641 tcctcatgtg catgatggat tggaaaataa tttctgaaga ctatactcgc taatggaaaa
    20701 gcaaagccga tgaagcgcaa taaattgcga gataaatctg atttgctagt atgcccgctt
    20761 cctcactatc ggagttaaca caaggatgag attaacgacg aaattttcgg cctttgttac
    20821 gctgctcacc gggttaacaa tttttgtgac tttgctgggc tgttcgctaa gtttctacaa
    20881 cgccattcag tataagttta gtcatcgtgt tcaggcggtg gcgacggcga tcgataccca
    20941 cctggtgtcg aatgacttca gcacattaag gccacaaatt accgaattaa tgatgtcggc
    21001 agatatcgtt cgtgtagacc tgctccatgg tgataagcag gtttataccc tggccagaaa
    21061 tggtagttat cgtccggttg gcaccaacga tctatttcgt gaactgagcg ttccgttgat
    21121 aaagcatccg gggatgtcgc tgcgtctggt ttatcaggat ccgatgggca actatttcca
    21181 ttcgttgatg accaccgcgc cgctcacggg ggcgattggc tttatcattc ttatgctctt
    21241 cctggcggta cgctggttac aacggcaact tgccgggcaa gaattgctgg aaacccgggc
    21301 tactcgtatc ttaaacggtg agcgtggctc taatgtgttg ggaaccatct atgaatggcc
    21361 gcccagaacc agcagtgcgc tggatacgct gcttcgtgaa attcagaacg cacgcgaaca
    21421 acacagccgt cttgatacgc tgatccgctc ttatgccgcc caggacatga aaaccggcct
    21481 caataaccga ctcttcttcg ataatcagtt agcaacgtta ctggaagatc aggagaaagt
    21541 aggtacccac gggatcgtga tgatgattcg tctgccggat ttcaatatgt tgagtgatac
    21601 ctgggggcac agccaggttg aagaacagtt cttctctctg acgaatctgc tgtcgacatt
    21661 tatgatgcgc taccctggcg cactgctggc gcgttaccac cgcagtgatt ttgctgcgct
    21721 gttaccgcac cgaacgttaa aagaggcaga gagcatcgcc agtcagttaa tcaaagccgt
    21781 cgataccttg ccgaacaata aaatgctcga tcgcgacgat atgatccaca ttggtatctg
    21841 tgcctggcgt agtggtcagg ataccgagca ggtaatggaa catgcagagt ctgccacgcg
    21901 taatgcggga ttgcagggcg gcaatagctg ggctatttac gatgactcgt tgcctgaaaa
    21961 aggacgcggt aatgttcgct ggcgtacgct tatcgagcaa atgctgagtc gcggcggccc
    22021 gcgcctttat caaaaaccgg cggttactcg cgaaggtcag gttcatcatc gcgaactcat
    22081 gtgccgcatc ttcgatggta atgaagaggt tagctcggcg gagtatatgc cgatggtctt
    22141 gcagtttggc ttatcggaag agtatgaccg cctgcaaatc agccgtctga ttccactatt
    22201 gcgttactgg ccggaggaaa atctggcgat tcaggttacc gttgagtcgc tgattcgccc
    22261 gcgttttcag cgttggctgc gcgatacgtt aatgcaatgt gaaagatcgc aacgaaaacg
    22321 cataattatt gaacttgcag aggccgatgt aggtcaacat atcagtcgct tacaacctgt
    22381 tattcgttta gtgaatgctt taggggtacg ggtagccgtc aaccaggctg gtttgacgct
    22441 ggtaagcacc agttggatca aagaacttaa tgttgagtta ctcaagctcc atccggggct
    22501 ggtcagaaac attgagaagc gaacggagaa ccagctgctg gttcaaagcc tggtggaagc
    22561 ctgctccggg accagcaccc aggtttacgc caccggcgtg cgttcgcgaa gcgagtggca
    22621 gaccctgatt cagcgcggtg ttacaggtgg gcaaggggat tttttcgcgt cctcacagcc
    22681 acttgatact aacgtgaaaa aatattcaca aagatactcg gtttaacctg ccgtttaatc
    22741 cgttttcacg tagaataacg cgcgctgcgt ctcatgggag tgtgcttgtc tgctcgccag
    22801 attgttgcag cacatatgca gatgaatgac cttacgcggt tgcaaacagg cgaggaatgc
    22861 tgctgatgca ttaagccttt ctggactcag gcagagattt gtaacaaagg aaacgaactg
    22921 cactaatttt caccgtagca gatgattttt gcgccttgtc gctgctgcgt gtggttggta
    22981 aagtaagcgg attttctttt ccgccccagc tttcaggatt atcccttagt atgttgaaaa
    23041 aatttcgtgg catgttttcc aatgacttgt ccattgacct gggtactgcg aataccctca
    23101 tttatgtaaa aggacaaggc atcgtattga atgagccttc cgtggtggcc attcgtcagg
    23161 atcgtgccgg ttcaccgaaa agcgtagctg cagtaggtca tgacgcgaag cagatgctgg
    23221 gccgtacgcc gggcaatatt gctgccattc gcccaatgaa agacggcgtt atcgccgact
    23281 tcttcgtgac tgaaaaaatg ctccagcact tcatcaaaca agtgcacagc aacagcttta
    23341 tgcgtccaag cccgcgcgtt ctggtttgtg tgccggttgg cgcgacccag gttgaacgcc
    23401 gcgcaattcg tgaatccgcg cagggcgctg gtgcccgtga agtcttcctg attgaagaac
    23461 cgatggctgc cgcaattggt gctggcctgc cggtttctga agcgaccggt tctatggtgg
    23521 ttgatatcgg tggtggtact actgaagttg ctgttatctc cttgaacggc gtggtttact
    23581 cctcttccgt gcgcattggt ggtgaccgtt tcgacgaagc tatcatcaac tatgtacgtc
    23641 gtaattacgg ttctctgatc ggtgaagcca ccgcagaacg tatcaagcac gaaatcggtt
    23701 cggcttaccc gggcgatgaa gtccgtgaaa tcgaagttcg tggccgtaac cttgcagaag
    23761 gtgttccacg cggttttacc ctgaactcca acgaaatcct cgaagcactg caggaaccgc
    23821 tgaccggtat tgtgagcgcg gtaatggttg cactggaaca gtgcccgccg gaactggctt
    23881 ccgacatctc cgagcgcggc atggtgctca ccggtggtgg cgcactgctg cgtaaccttg
    23941 accgtttgtt aatggaagaa accggcattc cagtcgttgt tgctgaagac ccgctgacct
    24001 gtgtggcgcg cggtggcggc aaagcgctgg aaatgatcga catgcacggc ggcgacctgt
    24061 tcagcgaaga gtaatcggat gcaggcaggg gaagcgtctg tttaccctgc ctggtctgat
    24121 acgagaatac gcataactta tgaagccaat ttttagccgt ggcccgtcgc tacagattcg
    24181 ccttattctg gcggtgctgg tggcgctcgg cattattatt gccgacagcc gcctggggac
    24241 gttcagtcaa atccgtactt atatggatac cgccgtcagc cctttctact ttgtttccaa
    24301 tgctcctcgt gaattgctgg atggcgtatc gcagacgctg gcctcgcgtg accaactaga
    24361 acttgaaaac cgggcgttac gtcaggaact gttgctgaaa aacagtgaac tgctgatgct
    24421 tggacaatac aaacaggaga acgcgcgtct gcgcgaactg ctgggttccc cgctgcgtca
    24481 ggatgagcag aaaatggtga ctcaggttat ctccacggtt aacgaccctt atagcgatca
    24541 agttgttatc gataaaggta gcgttaatgg cgtttatgaa ggccagccgg tcatcagcga
    24601 caaaggtgtt gttggtcagg tggtggccgt cgctaaactg accagtcgcg tgctgctgat
    24661 ttgtgatgcg acccacgcgc tgccaatcca ggtgctgcgc aacgatatcc gcgtaattgc
    24721 agccggtaac ggttgtacgg atgatttgca gcttgagcat ttgccagcga atacggatat
    24781 tcgtgttggt gatgtgctgg tgacttccgg tctgggcggt cgtttcccgg aaggctatcc
    24841 ggtcgcggtt gtctcttccg taaaactcga tacccagcgc gcttatactg tgattcaggc
    24901 gcgtccgact gcagggctgc aacgtttgcg ttatctgctg ctgctgtggg gcgcggatcg
    24961 taacggcgct aacccgatga cgccggaaga ggtgcatcgt gttgctaatg aacgtctgat
    25021 gcagatgatg ccgcaggtat tgccttcacc agacgcgatg gggccaaagt tacccgaacc
    25081 ggcaacgggg atcgctcagc cgactccgca gcaaccggcg acaggaaatg cagctactgc
    25141 gcctgctgcg ccgacacagc ccgctgctaa tcgctctcca caaagggcta cgccgccgca
    25201 aagtggtgct caaccgcctg cgcgtgcgcc gggagggcaa tagtggcgag ctatcgtagc
    25261 cagggacgct gggtaatctg gctctctttc cttattgcgc ttttgctgca aatcatgccc
    25321 tggccggaca acctgatagt tttccggcca aactgggtgt tactcatctt gttgtattgg
    25381 atcctggcct tgcctcatcg cgtaaatgtg ggcacaggtt ttgtgatggg tgccatactg
    25441 gatctgatca gcggctcgac acttggcgta cgcgtattgg cgatgagcat cattgcttac
    25501 ctggtggcgc tgaaatacca gcttttccgc aacctcgcat tatggcagca ggcgctggtc
    25561 gtcatgttgc tttcgctggt ggtggatatt attgttttct gggcagagtt tttagtgatt
    25621 aacgtctctt tcagaccgga agtgttctgg agtagtgtag tcaatggagt gctctggccg
    25681 tggattttct tgctgatgcg taaagtccgt cagcagtttg cagtgcaata aaggtttcta
    25741 tgacttctct gtatttagct tccggttctc cgcgtcgtca ggagttactt gcgcaacttg
    25801 gcgtaacctt tgaacgtatt gttacgggca ttgaggagca gcgtcagccg caggagagcg
    25861 cgcagcaata tgttgtgcgt ctggcgcgcg agaaagcaca ggcgggtgtc gcgcaaacgg
    25921 cgcaggatct cccggtgctg ggtgcggata ctatcgttat cctgaatgga gaagtgctgg
    25981 agaaaccgcg cgacgcagag catgcggtgc agatgttgcg caaattatcg ggtcagaccc
    26041 atcaggtaat gacggcagtg gcgttggccg acagccagca cattctcgat tgcctggtgg
    26101 tcaccgatgt gactttcaga acgttaacag acgaagacat cgcgggctat gtcgccagcg
    26161 gtgaaccgtt agataaagca ggtgcatacg gtattcaggg gctgggtggc tgttttgtca
    26221 ggaagataaa tggcagctat cacgccgtag tcggcttacc gctggttgaa acgtatgaat
    26281 tattaagtaa ttttaacgca ctgcgtgaga aaagggataa acatgacggc tgaattgtta
    26341 gtaaacgtaa cgccttcgga aacgcgagtg gcgtatattg atggcggtat tctgcaggaa
    26401 attcatattg aacgtgaggc gcgacgcgga atagtaggca atatctacaa gggtcgtgta
    26461 agtcgtgtac ttccgggtat gcaggcggct tttgtagata ttgggctgga taaagccgcg
    26521 tttcttcatg catccgacat catgccgcac accgaatgtg tggcgggtga agaacaaaag
    26581 caattcacgg tgcgcgacat ctcggagctg gtccgtcagg ggcaagatct gatggtgcag
    26641 gtggtgaaag atccgcttgg cactaaaggt gcgcgcctga ccaccgatat cacgctgcct
    26701 tctcgctatc tggtgtttat gccaggtgct tctcacgttg gggtatccca acgtattgaa
    26761 agcgaatcag aacgtgaacg cctgaaaaaa gtggtcgcag agtattgtga cgagcagggc
    26821 gggtttatca tccgtactgc agcggaaggg gttggcgagg ctgaactggc ctccgatgcc
    26881 gcttatctga aacgcgtctg gaccaaagtt atggagcgta aaaagcgccc acagacccgt
    26941 tatcagctgt acggcgaact ggcgctggcg cagcgtgttc tgcgtgattt cgccgacgcc
    27001 gaactcgacc gcattcgcgt tgactcacgc ctgacttacg aagcattgct ggagtttacc
    27061 tcggagtaca ttcccgagat gaccagcaag ctggagcatt acaccggacg ccagccgatt
    27121 ttcgatctct ttgatgtcga aaacgaaatt cagcgagcgc tggaacgcaa agtagaactg
    27181 aaatccggtg gctatctgat tatcgaccag accgaagcga tgaccaccgt ggacatcaat
    27241 accggggcgt ttgtcggtca tcgcaatctg gacgacacca ttttcaatac caatattgaa
    27301 gcgacgcagg ctatcgctcg ccagttacgg ttgcgcaatc tgggcgggat tatcattatt
    27361 gatttcatcg acatgaataa tgaagatcac cgtcgccgcg tgttgcattc gctggagcag
    27421 gcgttgagca aagaccgggt gaaaaccagc gttaatggtt tttcggcgct ggggctggtg
    27481 gagatgacgc gtaaacgcac ccgcgaaagc attgagcacg tactgtgtaa cgaatgccca
    27541 acctgccacg gtcgcggaac ggtgaaaacc gtggaaacgg tatgctatga aatcatgcgc
    27601 gagattgttc gtgtccacca tgcttacgac tccgaccgtt tcctggtcta tgcttctccg
    27661 gcagtagccg aagccttgaa aggcgaagag tcacactcgc tggcggaagt ggaaattttc
    27721 gttggcaaac aggttaaagt acaaattgaa ccgctctata accaggagca gtttgacgtc
    27781 gtaatgatgt aaacagatgc tgggccgcca tccggcaaag ggtttttgag tcacattttt
    27841 agcagacaag gagtgacggg tgaggcgatt gccggggatt ttactgctta ctggagctgc
    27901 gctcgttgtg atcgctgccc tgctggttag cggcctgcgt attgctttac cgcatcttga
    27961 cgcctggcgt ccggaaatcc tcaacaaaat agaatccgcg actggcatgc cggtagaagc
    28021 cagtcagctc tcagccagct ggcagaattt tggtccgacg cttgaagcac acgacatccg
    28081 tgcagaacta aaagatggcg gcgaattttc ggttaaacgc gttactcttg cgctagatgt
    28141 ctggcagagc ctgttacata tgcgctggca gtttcgcgac ctcactttct ggcatctgcg
    28201 ctttcgcacc aacactccta tcaccagcgg cggtggtaat gatagcctgg aagccagtca
    28261 catcagcgat ctgtttcttc gtcaatttga ccatttcgat ctccgcgaca gtgaagtcag
    28321 tttcctgacg ccatccggtc agcgcgccga gctggcgatc ccacagctca cctggctgaa
    28381 cgatccacgt cgacaccgtg cggaaggcct ggtaagcctc tccagcctta ccggacagca
    28441 cggcgtgatg caggtgcgta tggatttgcg cgatgatgag gggttgttaa gcaatggtcg
    28501 cgtctggctc caggcggatg acatcgacct gaagccgtgg ctcggtaaat ggatgcagga
    28561 caatattgcg ctggaaacgg cacagttctc ccttgaaggc tggatgacga tcgacaaagg
    28621 cgatgtaacc ggcggtgacg tctggctgaa acagggcggt gccagctggt tgggcgagaa
    28681 ggaaacacat acgctgtcgg tggataatct gactgcgcat attacgcgtg aaaatccggg
    28741 atggcagttc tctattcccg atacacggat cacgatggac ggcaaaccct ggccgagcgg
    28801 agcattgacg ctggcctgga taccggaaca ggacgttggc ggcaaagaca ataaacgcag
    28861 tgacgaactc cggattcgtg ccagtaatct ggagctggca ggcctggagg gcgtacgccc
    28921 gttggccgcg aaactttcac ctgcactggg tgatgtttgg cgctccacac aaccgagcgg
    28981 caagattaac actctggcgc tggatatccc gcttcaggcg gcagacaaga cccgttttca
    29041 ggcatcgtgg agcgatctgg cctggaagca atggaaatta ttaccgggtg cggaacactt
    29101 ctccgggacg ctttccggca gcgtagaaaa tggtttgctt actgcgtcga tgaagcaggc
    29161 aaagatgcct tacgaaacgg tattccgcgc gccactggaa atcgccgacg gccaggcaac
    29221 gataagctgg ctgaacaatg acaaaggttt ccagctggat ggacgtaata ttgacgttaa
    29281 agccaaagcc gtccatgcgc gcggcggttt tcgttacctg caacctgcta acgatgaacc
    29341 ctggctgggg attctggctg gcatcagtac cgacgatggt tcacaagcct ggcgctattt
    29401 cccggaaaac ttgatgggta aagacctggt tgattactta agtggcgcga ttcagggcgg
    29461 tgaagcggat aacgcgacgc tggtttatgg tggcaatccg caactcttcc cctataaaca
    29521 caacgaaggt cagtttgaag tgctggtgcc gctgcgcaac gcgaagtttg ccttccagcc
    29581 ggactggcct gcattaacta accttgatat tgaactggac tttattaacg acggtttatg
    29641 gatgaaaacc gatggcgtta atctgggcgg cgtgcgtgcg agtaatctca ccgcagtgat
    29701 ccctgactac tcaaaagaaa aactgctgat tgacgctgac attaaaggcc cgggtaaagc
    29761 cgttggccct tactttgatg agacaccgct gaaagattct ctgggggcga ccctgcaaga
    29821 actccagctc gacggcgatg tgaatgctcg cttacatctc gatattccgc tgaacggcga
    29881 actggtaacc gcgaaaggtg aagtgacgct gcgtaataac agtctgttta tcaaaccact
    29941 cgacagcacc ctgaaaaatt tgagcggtaa attcagcttt atcaatggcg atctgcaaag
    30001 tgaaccactg acagcaagct ggtttaatca gccgttgaac gtggattttt ccaccaaaga
    30061 aggggcaaaa gcctaccagg tcgcggtgaa tctcaacggt aactggcaac cagcgaaaac
    30121 cggcgttctg cctgaagcgg tgaacgaagc attaagtggc agcgtggcgt gggatggtaa
    30181 agtgggcatt gagctgcctt atcatgctgg cgcgacgtat aacgtagagc tgaatggcga
    30241 tttgaagaat gtgagcagtc acttaccttc accgttagcc aaacctgcgg gtgaaccact
    30301 accggtaaac gttaaggttg atggcaatct caacagcttt gaattaaccg gacaggctgg
    30361 tgcggataac catttcaata gccgctggtt gctcggtcaa aagctgacgc tcgatcgtgc
    30421 tatatgggcg gcagacagta aaacgctccc gccgttgccg gaacaaagtg gcgttgaact
    30481 caatatgccg ccgatgaatg gtgccgagtg gctggccctg ttccagaaag gcgcggcgga
    30541 gagtgtcggt ggtgcagcga gtttcccaca acacataacg ttacgtacgc ctatgttgtc
    30601 gctgggaaat cagcaatgga ataacctgag cattgtttcg caaccgacgg caaatggcac
    30661 ccaggttgag gcgcaagggc gtgaaatcaa cgccacgctg gcgatgcgta ataacgcgcc
    30721 gtggctggcg aatatcaaat atctttatta caacccaagc gtgccgaaaa ctcatggtga
    30781 ctcaacgaat tcatcacctt tcctgacaac ggagcgcatt agcttccgtg gctggccgga
    30841 cgcacaaata cgatgcgcag agtgctggtt ctgggggcaa aaattcgggc gcattgacag
    30901 tgatatcacc atttctggca atacattaac gctgactaat ggactgattg atactggttt
    30961 ttcgcggctc actgccgatg gtgaatgggt taacaatccg ggaaatgaac gcacttcgct
    31021 gaaaggaaaa ctgcgtgggc agaaaattga tgccgccgca gaattttttg gtgtcacgac
    31081 gcccatacgg cagtcgtcat ttaatgtgga ttacgattta cactggcgca aagcaccctg
    31141 gcaaccagat gaggcgacgt tgaatggcat cattcatact caactgggca aaggcgaaat
    31201 taccgaaatc aataccggac atgccgggca attgctgcgc ttattgagcg ttgatgccct
    31261 gatgcgtaag ctgcgttttg atttcagaga cacttttggc gaagggttct attttgactc
    31321 cattcgcagc accgcgtgga ttaaagacgg cgttatgcac accgacgaca cgctggtgga
    31381 tggcctggaa gcggacatcg ctatgaaagg gtcggtaaat ctggtacgtc gcgacctgaa
    31441 tatggaagcg gttgtcgcac cagagatttc tgcgacggtg ggtgtggctg cggcctttgc
    31501 ggttaacccc attgttggcg cggcagtgtt tgccgccagt aaagtgctgg gaccgctgtg
    31561 gagcaaagtc tccattttgc gctatcacat ttcgggtccg ctggacgatc cgcaaatcaa
    31621 cgaagtgttg cgacaaccgc gtaaagaaaa agcgcaatga tttgacgagg gcgcgtaatt
    31681 gccccaatct cataggataa tcgttgccaa aggccaacga gccagaacat aaccgtaggc
    31741 cggatagggc gttcacgccg catccggcag ccgtaaaaaa tcctctactg cagtaactaa
    31801 cgagtagcaa aaacgatgag tcttaacctg gtaagtgaac aattgctagc ggcgaacggc
    31861 ctgaaacatc aggacttgtt cgcgatcctc ggtcaactgg ccgaacgtcg ccttgattat
    31921 ggcgatctct attttcagtc gagctatcac gaatcctggg ttttagaaga ccgcattatt
    31981 aaagatggtt cttacaacat cgatcagggc gttggtgtgc gtgcaatcag cggtgaaaaa
    32041 accggatttg cttacgctga ccaaatcagc ctgctggcgc tggaacaaag tgcgcaagcg
    32101 gcgcgcacca tcgtccgtga tagtggtgat ggcaaagtac agacgctggg cgcggtagag
    32161 catagcccgt tgtatacctc ggtagatccg ctgcaaagca tgagccgtga agagaagctg
    32221 gatatcctgc gtcgcgtcga taaggttgcc cgcgaagcgg acaagcgcgt acaagaagtg
    32281 actgccagcc tcagcggcgt ttatgaatta attctggttg cggccaccga cggcacgctg
    32341 gcggcggatg ttcgtccgct ggtgcgtctt tccgtgagcg ttctggtcga agaagatggc
    32401 aaacgcgaac gcggtgccag tggcggcggc ggtcgttttg gttatgagtt cttccttgcc
    32461 gatctcgacg gtgaagtccg tgcggatgca tgggcaaaag aagctgtgcg tatggcgctg
    32521 gtcaatcttt ctgccgtcgc cgcaccagca ggcaccatgc cggtagtact tggcgcaggt
    32581 tggccgggcg tgctgttgca tgaagcggtg ggtcacggtc tggaaggcga cttcaaccgc
    32641 cgtggcactt cagtatttag tggacaggtc ggggagctgg tggcttcaga actgtgtacc
    32701 gtggttgatg acggtacgat ggtcgatcgc cgtggttcgg tggcgattga tgacgaaggt
    32761 acgccaggcc agtacaacgt gctgattgag aacggcattc tgaaaggcta catgcaggat
    32821 aaactcaacg cgcgtttgat ggggatgacg ccgactggca acggtcgccg tgaatcctac
    32881 gcccatctgc ccatgccgcg tatgaccaac acctatatgc tgccgggtaa atcgaccccg
    32941 caggaaatta ttgaatccgt tgagtacggt atctatgcac cgaactttgg tggcggtcag
    33001 gtggatatca cctccggcaa attcgttttc tccacttcag aagcgtatct gattgaaaac
    33061 ggtaaagtaa cgaagccggt gaaaggcgca acgttgattg gttccggtat cgaaaccatg
    33121 cagcagattt cgatggttgg caacgacctg aaactggata acggcgtggg tgtctgcggt
    33181 aaagaagggc aaagtttgcc ggttggcgtg ggccagccaa cgctgaaagt tgataacctg
    33241 actgttggcg gtactgcgta ataatttcag tattttcaga aggataatta atccttctcc
    33301 gtgtacgaac ggtcccctcg cccctctggg gttagggtga ggggaacccg ttggcacagg
    33361 tttgtactac gtaacagtac aatattaatt acttctcttt cccgcgcccg tgcatctcct
    33421 gaaacaattt accgacctca acaaaataat ccgtcagcga gttgatcacg acctgtacct
    33481 tcagcggtag cttatctttt tcggtataca acgcataaac cgggcgtgga tctgactggt
    33541 aacgcggcag caggatctcc agttccccgc gattgatctc gttgatcacc cacatcagcg
    33601 gcacgtaggc gatcccggca cccgccgtca gccagcgcac cagcgtcatc ggatcattag
    33661 tcacaaatct tccttgtggg atcaggcgag tcgagatccc ttccggtgcg atcagttcaa
    33721 attcattgtc gggccgcacg ctgtattcaa gccatgaatg actactcaaa tcggcgggtt
    33781 tttccggtat gccgtattgt gtgagatagc ttttcgcggc gcacaccacc atcggcatcg
    33841 cgcccagacg gcgggaaaac aggctggaat cctgcaacgc gccgacgcgg atcaccacat
    33901 ccagaccatc ggcaatcagg tcgggggcag gaattccggt aaccagattg acgctcaaac
    33961 ctgggtattc tttcagcatt tttgctgtca gcccggcgag aacattttgt gccatagttg
    34021 aagaacagcc aatgcgcagc gtcccgatgg gggtgttatt gaaggcatac agttgctcat
    34081 gaacatcctg cacttcatgg agcatccgac ggcagccctg gtagtaaatt ctaccggctt
    34141 cggtcaggcc aatgctgcgt gtgctacggt ttaacagctt tacctgtaac tcatcttcca
    34201 gttttgacac cgtctgactg atggacgaaa cgctcatctg tagctgtctg gcggcggcgg
    34261 taaaagagcc aaattcaact actttggcaa acaccgacat gcgttttagt cgttccatta
    34321 ttcactctga cttaaaagtg atttagatca cataatatag ataacagcat aacagttacg
    34381 ctaatatatt aaatatcaat ctacagcaat gttgctctcg cccggctttc catgcccttc
    34441 tctgcggcga cagatgctga aaataataac gcctgctctc tctatacaac caaggtcaac
    34501 atgagtctgt ttcccgttat cgtggtgttt gggctgtcct tcccaccgat attttttgaa
    34561 ttgcttttat cactggcgat tttctggctg gtgcgccggg tacttgtgcc aacaggtatc
    34621 tacgactttg tctggcatcc ggcgttgttc aacaccgcgc tctattgctg cttgttttat
    34681 ttgatatcgc gactgttcgt ttgaggttga agtgaaaaca ctaataagaa aattctcccg
    34741 tacggccatc acggtcgtat tagtcattct ggccttcatc gcaattttta atgcctgggt
    34801 ctattacacc gaatccccgt ggacgcgtga cgcgcgcttt agcgctgacg tcgttgcgat
    34861 cgcgccggac gtttctggac tcattaccca ggtgaatgtt catgataacc agctggtgaa
    34921 aaaaggacag gtactgttca ccatcgacca gccgcgttat caaaaggcgc ttgaggaagc
    34981 gcaagccgat gttgcttatt atcaggtact ggcacaggag aaacgccagg aggccggacg
    35041 tcgtaaccgt ctcggtgtgc aggcgatgtc tcgcgaagag atcgaccagg ccaacaacgt
    35101 actacaaaca gttctgcatc agttagcgaa agcgcaggcg acccgcgatc tggcaaaact
    35161 ggatcttgaa cgtacagtca tccgcgcgcc agcagatggc tgggtgacca acctcaacgt
    35221 ctataccggt gagtttatta cccgtggatc aacggcggtt gcgctggtga aacagaactc
    35281 cttctatgta ctggcctata tggaagaaac taagctggaa ggggtgcgcc ctgggtatcg
    35341 tgcggagatc acgccgcttg gcagtaacaa agtgctgaaa gggactgttg atagtgttgc
    35401 cgctggggtc accaacgcca gcagcacgcg tgacgacaaa gggatggcga ccatcgactc
    35461 taaccttgaa tgggtgcgtt tggcgcaacg tgttccggtt cgtattcgtc tcgacaacca
    35521 gcaagagaac atctggcctg cgggcaccac tgctacagtg gtggtcactg gcaaacaaga
    35581 tcgcgacgaa agccaggatt cgttcttccg taaaatggcc catcgcctgc gtgagtttgg
    35641 ttaatcacga tgggtatttt ctccattgct aaccaacata ttcgctttgc ggtaaaactg
    35701 gcgaccgcca ttgtactggc gctgtttgtt ggctttcact tccagctgga aacgccacac
    35761 tgggcggtac tgacagcggc gattgttgcc gccggtccgg cctttgctgc gggaggtgaa
    35821 ccgtattctg gcgctattcg ctatcgtggc tttttgcgca tcatcggcac atttattggc
    35881 tgtattgccg gactggtaat catcattgcg atgatccgcg caccattatt gatgattctg
    35941 gtgtgctgta tctgggccgg tttttgtacc tggatatcct cgctggtacg aatagaaaac
    36001 tcgtatgcgt gggggctggc cggttatacc gcgctgatca ttgtgatcac tattcagccg
    36061 gaaccattgc ttacgccgca gtttgccgtc gaacgttgta gcgagatcgt tatcggtatt
    36121 gtgtgtgcaa ttatggcgga tttgctcttt tctccgcgat cgatcaaaca agaagtggat
    36181 cgagagctgg aaagtttgct ggtcgcgcaa tatcaattaa tgcaactctg tatcaaacat
    36241 ggcgatggtg aagttgtcga taaagcctgg ggcgatctgg tgcgacgtac tacggcgtta
    36301 caaggtatgc gcagcaacct gaatatggaa tcttcccgct gggcgcgggc caatcgacgt
    36361 ttaaaagcga tcaatacgct atcgctgacg ctgattacac aatcctgcga aacttatctt
    36421 attcagaata cgcgcccgga attgatcact gatactttcc gcgaattttt tgacacgccg
    36481 gtagaaaccg cgcaggacgt ccacaagcag ctcaaacgcc tgcggagagt gatcgcctgg
    36541 accggggaac gggaaacgcc tgttaccatt tatagctggg tcgcggcggc aacgcgttat
    36601 cagcttctca agcgcggcgt tatcagtaac acaaaaatca acgccaccga agaagagatc
    36661 ctgcaaggcg aaccggaagt caaagtagag tcagccgaac gtcatcatgc gatggttaac
    36721 ttctggcgaa ccacactttc ctgcattctg gggacgcttt tctggctgtg gacgggctgg
    36781 acttccggca gtggtgcaat ggtgatgatt gcggtagtga cgtcactggc aatgcgtttg
    36841 ccaaatccac gcatggtggc gatcgacttt atctacggga cgctggccgc gctgccgtta
    36901 gggctgctct actttttggt gattatccct aatactcaac agagcatgtt gctgctgtgt
    36961 attagcctgg cagtgctggg atttttcctc ggtatagaag tacagaaacg gcgactgggc
    37021 tcgatggggg cgctggccag caccataaat attatcgtgc tggataaccc gatgactttc
    37081 catttcagtc agtttctcga cagcgcatta gggcaaatcg tcggctgtgt gctcgcgttc
    37141 accgttattt tgctggtgcg ggataaatcg cgcgacagga ctggacgtgt actgcttaat
    37201 cagtttgttt ctgccgctgt ttccgcgatg actaccaatg tggcacgtcg taaagagaat
    37261 cacctcccgg cactttatca gcagctgttt ttgctgatga ataagttccc aggggatttg
    37321 ccgaaatttc gcctggcgct gacgatgatt atcgcgcacc agcgcctgcg tgatgcgccg
    37381 atcccggtta acgaggattt atcggcgttt caccgacaaa tgcgccgcac agcagaccat
    37441 gtgatatctg cccgtagcga tgataaacgt cgtcggtact ttggccagtt gctggaagaa
    37501 cttgaaatct accaggaaaa gctacgcatc tggcaagcgc caccgcaggt gacggaacca
    37561 gtacatcggc ttacgggtat gctccataag tatcaacatg cgttgaccga tagttaagtc
    37621 aaaaccgacg ccaaaagcgt cggttttttc atggctatac ttagcaatga acggcagaac
    37681 tcgccgcgaa acgtgacggt ggcaacagat gaatatttat acctttgatt ttgatgagat
    37741 tgagagtcag gaggattttt atcgcgactt cagccaagcc tttggtctgg cgaaagataa
    37801 ggtacgcgat ctcgactcac tatgggatgt gttaatgaac gatgtcctgc cgctaccact
    37861 tgagattgaa tttgttcatc tgggagagaa aacgcgtcgc cgttttggcg cgttaatatt
    37921 gctgtttgat gaggcagagg aagagctgga agggcatttg cgttttaatg ttcgtcatta
    37981 gcgcaaaaaa aagcccccga accgggggca atatcgtcgg acaagacgat gagggtttat
    38041 ttgtacagtt cagccgtagc gtgccaggtg tcaccgctac gagcttccgt aatctggtag
    38101 gccgttgcgc ctttctcttc tgcttttttg ttcagcattt cacgcatatc cattggcgaa
    38161 gacgccacac cacttacgga tacggtcccg attgcttcac gattttgtgc ttgtgcagca
    38221 tcaatggagt cggcagcgaa tgcaccgaaa gagagaacag aaagtacgct taatgcagca
    38281 acagtggttt tgattttcat gatttttacc tcgacataat cttttagctg ggcctttgtt
    38341 tcgtgaccca tatcacaaaa tcaagtatac actaattatt gaactaatta ataccacgct
    38401 aactgtttca gttgatacta agtgttaaaa atgtgaattt taataacaaa aaagtattaa
    38461 aaacacggtt atttattata aataccatat ttttcaatta gataatggat tttgcataat
    38521 gtgctttttt taccgtatgt attcattgtg tgaatgatat gtcgcagtaa aacgcactat
    38581 tcgttaaatt aatcgcaggg gaaagcaggg ggttgagagg gataagcaac attttccccg
    38641 ccgccagaag cgacggggca gagattaaag ctcctggtcg aacagctcta aaatcgcttc
    38701 gtacaggtct ttgacggtga aaccgttagc aggggtggta aagatggtgt catcgccagc
    38761 gatggtgccc agaatacctt ctgctttgcc cagtgaatcc agcaggcgag caattaactg
    38821 cgccgcgcca gggctggtat gaatcacgac aactgcatcg ttgtagtcga tatccagcac
    38881 cagatttttc aatggactgg aggtggttgg tacacccagt tcagctggca ggcagtaaac
    38941 catttccatt ttggcattgc gtgtacgtac agcaccaaac ttggtcaaca tccgcgagac
    39001 tttagactga ttaatattgt caaagccttg ctcctgcaac gcggcgacga tttcgccctg
    39061 ggagctaaat ttctcttctt taagtaatgc tttaaatgct ttaactagtt cttcttgctt
    39121 agccgagctt cgcataagtc acccgatatg gtggttgata caacattatt gtgcatacag
    39181 atgaattttt atgcaaacag tcagccctga agaaggctga aataatgtta tgaaagagcg
    39241 ggattttatc aaatttcgtt attgagaaac atgcctgcgt cacggcatgc aaattctgct
    39301 taaaagtaaa ttaattgtta tcaaattgat gttgttttgg ctgaacggta gggtatattg
    39361 tcaccacctg ttggaatgtt gcgctaatgc ataagcgact gttaattacg taagttaggt
    39421 tcctgattac ggcaattaaa tgcataaacg ctaaacttgc gtgactacac attcttgaga
    39481 tgtggtcatt gtaaacggca attttgtgga ttaaggtcgc ggcagcggag caacatatct
    39541 tagtttatca atataataag gagtttagga tgaaagtcgc agtcctcggc gctgctggcg
    39601 gtattggcca ggcgcttgca ctactgttaa aaacccaact gccttcaggt tcagaactct
    39661 ctctgtatga tatcgctcca gtgactcccg gtgtggctgt cgatctgagc catatcccta
    39721 ctgctgtgaa aatcaaaggt ttttctggtg aagatgcgac tccggcgctg gaaggcgcag
    39781 atgtcgttct tatctctgca ggcgtagcgc gtaaaccggg tatggatcgt tccgacctgt
    39841 ttaacgttaa cgctggcatc gtaaaaaacc tggtacagca agttgcgaaa acttgcccga
    39901 aagcgtgcat tggtattatc actaacccgg ttaacactac agtggcgatt gctgctgaag
    39961 tgctgaaaaa agccggtgtt tatgacaaaa acaaactgtt cggcgttacc acgctggata
    40021 tcattcgttc caacaccttt gttgcggaac tgaaaggcaa acagccaggc gaagttgaag
    40081 tgccggttat tggcggtcac tctggtgtta ccattctgcc gctgctgtca caggttcctg
    40141 gcgttagttt taccgagcag gaagtggctg atctgaccaa acgtatccag aacgcgggta
    40201 ctgaggtggt tgaagcgaaa gccggtggcg ggtctgcaac cctgtctatg ggtcaggcag
    40261 ctgcacgttt tggtctgtct ctggtacgcg cactgcaggg cgaacaaggc gttgtcgaat
    40321 gtgcctacgt tgaaggtgac ggtcagtacg cacgtttctt ctctcaaccg ctgctgctgg
    40381 gtaaaaacgg cgtggaagag cgtaaatcta tcggcactct gagcgcattt gaaaagaacg
    40441 cgctggaagg tatgctggat acgctgaaga aagatatcgc cctgggcgaa gagttcgtta
    40501 ataagtaatt gattagcgga taataaaaaa ccggagcaca gactccggtt ttttgttttg
    40561 agcgcacgac ttaattggtt gccggatatt cctgaatggt gacctgcagc gttaactgct
    40621 tatcatcacg catcactaca accgggatca ccgagccagg gcgaatttcc gccacctgat
    40681 ccatcgtctc cagagcagag atggccggtt tgttatccac cgaaataatc aaatcattga
    40741 cctgaatacc cgcattcgcc gccgggccgt caggtgacac ttcattaacc acgatccctt
    40801 gcagttgatc tataccaccg ccctgcgcgt gcagtggtgc gatctcacgt ccgccgatac
    40861 caatgtagcc gcggatcacg cgaccatcgc ggatcagttt atccataatt ttggttgcta
    40921 actggaaagg aatcgcaaag ccgatacctt ccggcgtttc gccatcgtta ctcttatcaa
    40981 acgacagcgt gttaatgccc atcagttcgc ccagcgagtt caccagcgcg ccgccagagt
    41041 taccgtggtt aatggaagca tcggtttgca ggaagttttg ccgcccggtc gggttcagac
    41101 cgattcgacc cgtggcacta ataatcccct gggtaatggt ctgcccgagg ttgtacgggt
    41161 taccgatcgc cagtaccacg tcaccaatgt gcggtacgcg acgtgcatta attggaatgg
    41221 taggtaaacc gccagtggca ttaattttaa gtaccgccag atcggttaga gagtcagatc
    41281 ccaccagcaa tgcttcaaat acacgtccat cttgtaaggc gacgatgatc tgatcggcgt
    41341 cgttgatgac gtgtttattg gtgatgatat aaccgcgttg atccatgatt acaccggatc
    41401 ccagggtgcg gatctcaagc tggttgtgag agttggtgtt caaaccacgg ttgtaaacgt
    41461 taaccaccgc tggcgcggcg cggcgaaccg ccagattata gctggcaggc gtctcatcgg
    41521 tactgtcaaa ttgcggagtg gaaagcgggt taaggctgcg cagcgaaggc atggcaacca
    41581 gcagaatagc gccgacaatt aatccaatcg caacggaacg taagagcttc acaaacatga
    41641 tggaggcatc gttaaaaaag ggaacggcag cagcatacca cgagttaacc ggacatcaca
    41701 cgtcagcctg atgcccggtt tacgacatta acgcatcagc agatagatgc tttcattgcc
    41761 gcgtacaatt tgcagggcga tgatggccgg ttttgccgcc agcactttac gcatttcagc
    41821 aatcgagttc acccgatcgc ggttgacgcc aatgatcaca tcgtcttttt gcaagccagc
    41881 ctgagcagct gggcttcctt tgacaacctc atcgatctta atacctttgc cgccatcttt
    41941 tagctgacca tcgctcaacg ttgcaccttc cagcgctggc gtgatcattt cagcgctggc
    42001 cgacgaagag gtgctggtat caagcgtcac ttctacttcc agtggtttgc cgttacgcag
    42061 caggccaagt ttcacttttg tgcccggctc ggtggtcgcg atacgagagc gcaactcagc
    42121 aaagctattc agcggtttgc cgttgaggct ggtaataata tcgcccgctt tgatgcccgc
    42181 ttttgccgag ccagaacctg gcaacacttc gctgacaaac gcgccacgct gcacgtcaag
    42241 gttgaaggct ttggcgatat cggcactcat ctctgtgcct ttgatgccta acagaccgcg
    42301 tttgatttca ccgaagtcga taagctgctg cgccagtgtt cgcgccatat tactggggat
    42361 ggcaaatcca atcccgacgc tcccaccgcc aggcgcaagg attgcagtgt tgatgccaat
    42421 taactcaccg ttaaggttta acagtgcacc gccggagtta ccgcggttaa tggaagcatc
    42481 tgtctggata aagttttcaa gaccttcaag attcaacccg ctgcggccta atgcggaaac
    42541 aatgccagag gtggcggttt gcccaaggcc aaatgggtta ccgaccgcca cggcaaaatc
    42601 accgac
//
LOCUS       DAMZFT010000024        42305 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.024, whole genome shotgun sequence.
ACCESSION   DAMZFT010000024 DAMZFT010000000
VERSION     DAMZFT010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 42305)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42305)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42305
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.024"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(238..1611)
                     /gene="proY"
                     /locus_tag="PN977_001459"
     CDS             complement(238..1611)
                     /gene="proY"
                     /locus_tag="PN977_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline-specific permease ProY"
                     /protein_id="HDI6041507.1"
                     /translation="MESKNKLKRGLSTRHIRFMALGSAIGTGLFYGSADAIKMAGPSV
                     LLAYIIGGIAAYIIMRALGEMSVHNPAASSFSRYAQENLGPLAGYITGWTYCFEILIV
                     AIADVTAFGIYMGVWFPTVPHWIWVLSVVLIICAVNLMSVKVFGELEFWFSFFKVATI
                     IIMIVAGFGIIIWGIGNGGQPTGIHNLWSNGGFFSNGWLGMVMSLQMVMFAYGGIEII
                     GITAGEAKDPEKSIPRAINSVPMRILVFYVGTLFVIMSIYPWNQVGTAGSPFVLTFQH
                     MGITFAASILNFVVLTASLSAINSDVFGVGRMLHGMAEQGSAPKIFSKTSRRGIPWVT
                     VLVMTTALLFAVYLNYIMPENVFLVIASLATFATVWVWIMILLSQIAFRRRLPPEEVK
                     ALKFKVPGGVATTIGGLIFLLFIIGLIGYHPDTRISLYVGFAWIVVLLIGWMFKRRHD
                     RQLAENQ"
     gene            complement(1687..3006)
                     /gene="brnQ"
                     /locus_tag="PN977_001460"
     CDS             complement(1687..3006)
                     /gene="brnQ"
                     /locus_tag="PN977_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transporter carrier
                     protein BrnQ"
                     /protein_id="HDI6041508.1"
                     /translation="MTHQLRSRDIIALGFMTFALFVGAGNIIFPPMVGLQAGEHVWTA
                     AFGFLITAVGLPVLTVVALAKVGGGVDSLSTPIGKVAGVLLATVCYLAVGPLFATPRT
                     ATVSFEVGIAPLTGDSALPLFIYSLVYFAIVILVSLYPGKLLDTVGNFLAPLKIIALV
                     ILSVAAIIWPAGSISTATEAYQNAAFSNGFVNGYLTMDTLGAMVFGIVIVNAARSRGV
                     TEARLLTRYTVWAGLMAGVGLTLLYLALFRLGSDSASLVDQSANGAAILHAYVQHTFG
                     GGGSFLLAALIFIACLVTAVGLTCACAEFFAQYVPLSYRTLVFILGGFSMVVSNLGLS
                     QLIQISVPVLTAIYPPCIALVVLSFTRSWWHNSSRVIAPPMFISLLFGILDGIKASAF
                     SDILPSWAQRLPLAEQGLAWLMPTVVMVVLAIIWDRAAGRQVTSSAH"
     gene            complement(3413..4708)
                     /gene="phoR"
                     /locus_tag="PN977_001461"
     CDS             complement(3413..4708)
                     /gene="phoR"
                     /locus_tag="PN977_001461"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon sensor histidine kinase PhoR"
                     /protein_id="HDI6041509.1"
                     /translation="MLERLSWKRLVLELLLCCLPAFILGAFFGYLPWFLLASVTGLLI
                     WHFWNLLRLSWWLWVDRSMTPPPGRGSWEPLLYGLHQMQLRNKKRRRELGNLIKRFRS
                     GAESLPDAVVLTTEEGGIFWCNGLAQQILGLRWPEDNGQNILNLLRYPEFTQYLKTRD
                     FSRPLNLVLNTGRHLEIRVMPYTHKQLLMVARDVTQMHQLEGARRNFFANVSHELRTP
                     LTVLQGYLEMMDEQPLEGAVREKALHTMREQTQRMEGLVKQLLTLSKIEAAPTQLLNE
                     KVDVPMMLRVVEREAQTLSQKKQTFTFEIDNGLKVSGSEDQLRSAISNLVYNAVNHTP
                     EGTHITVRWQRVPHGAEFSVEDNGPGIAPEHIPRLTERFYRVDKARSRQTGGSGLGLA
                     IVKHAVNHHESRLNIESTVGKGTRFSFVIPERLIAKNSD"
     gene            complement(4766..5455)
                     /gene="phoB"
                     /locus_tag="PN977_001462"
     CDS             complement(4766..5455)
                     /gene="phoB"
                     /locus_tag="PN977_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000113934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate response regulator transcription
                     factor PhoB"
                     /protein_id="HDI6041510.1"
                     /translation="MARRILVVEDEAPIREMVCFVLEQNGFQPVEAEDYDSAVNQLNE
                     PWPDLILLDWMLPGGSGIQFIKHLKRESMTRDIPVVMLTARGEEEDRVRGLETGADDY
                     ITKPFSPKELVARIKAVMRRISPMAVEEVIEMQGLSLDPTSHRVMAGEEPLEMGPTEF
                     KLLHFFMTHPERVYSREQLLNHVWGTNVYVEDRTVDVHIRRLRKALEPGGHDRMVQTV
                     RGTGYRFSTRF"
     gene            5645..6847
                     /gene="sbcD"
                     /locus_tag="PN977_001463"
     CDS             5645..6847
                     /gene="sbcD"
                     /locus_tag="PN977_001463"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcD"
                     /protein_id="HDI6041511.1"
                     /translation="MRILHTSDWHLGQNFYSKSREAEHQAFLDWLLETAQTHQVDAII
                     VAGDVFDTGSPPSYARTLYNRFVVNLQQTGCHLVVLAGNHDSVATLNESRDIMAFLNT
                     TVVASAGHAPQILPRRDGTPGAVLCPIPFLRPRDIITSQAGLNGIEKQQHLLAAITDY
                     YQQHYADACKLRGDQPLPIIATGHLTTVGASKSDAVRDIYIGTLDAFPAQNFPPADYI
                     ALGHIHRAQIIGGMEHVRYCGSPIPLSFDECGKSKYVHLVTFSNGKLESVENLNVPVT
                     QPMAVLKGDLASITEQLEQWRDVSQEPPVWLDIEITTDEYLHDIQRKIQALTESLPVE
                     VLLVRRSREQRERVLASQQRETLSELSVEEVFNRRLALEELDESQQQRLQHLFATTLH
                     SLAGEHEA"
     gene            6844..9987
                     /gene="sbcC"
                     /locus_tag="PN977_001464"
     CDS             6844..9987
                     /gene="sbcC"
                     /locus_tag="PN977_001464"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcC"
                     /protein_id="HDI6041512.1"
                     /translation="MKILSLRLKNLNSLKGEWKIDFTREPFASNGLFAITGPTGAGKT
                     TLLDAICLALYHETPRLSNVSQSQNDLMTRDTAECLAEVEFEVKGEAYRAFWSQNRAR
                     NQPDGNLQVPRVELARCADGKILADKVKDKLELTATLTGLDYGRFTRSMLLSQGQFAA
                     FLNAKPKERAELLEELTGTEIYGKISAMVFEQHKSARTELEKLQAQASGVALLTPEQV
                     QSLTASLQVLTDEEKQLITAQQQEQQSLNWLTRLDELQQEGSRRQQALQQALAEEEKA
                     QPQLAALSLAQPARNLRPHWERIAEHSAALAHTRQQIEEVNTRLQSTMALRASIRHHA
                     AKQSAELQQQQQSLNAWLQEHDRFRQWNNELAGWRAQFSQQTSDREHLRQWQQQLTHA
                     EQKLNALAAITLTLTADEVASALAQHAEQRPLRQRLVALHGQIVPQQKRLAQLQVAIQ
                     NVTLEQTQRNAALNEMRQRYKEKMQQLADVKTICEQEARIKTLEAQRAQLQAGQPCPL
                     CGSTSHPAVEAYQALEPGVNQARLLTLEKEVKKLGEEGAALRGQLDALTKQLQRDENE
                     AQSLRQDEQALTQQWQAVTASLNITLQPQDDIQPWLDAQDEHERQLRLLSQRHELQGQ
                     IAAHNQQIIQYQQQIEQRQQQLLTALAGYALTLPQEDEEESWLAARQQEAQRWQQRQN
                     ELTALQNRIQQLTPILETLPQSDELPHCEETVVLENWRQVHEQCLALHSQQQTLQQQD
                     VLAAQSLQKAQAQFDTALQASVFDDQQAFLAALMDEQTLTQLEQLKQNLENQRRQAQT
                     LVTQTAEALAQHQQHRPDGLDLSVTVEQIQQELAQTQQKLRENTTSQGEIRQQLKQDA
                     DNRQQQQTLMQQIAQMTQQVEDWGYLNSLIGSKEGDKFRKFAQGLTLDNLVHLANQQL
                     TRLHGRYLLQRKASEALEVEVVDTWQADAVRDTRTLSGGESFLVSLALALALSDLVSH
                     KTRIDSLFLDEGFGTLDSETLDTALDALDALNASGKTIGVISHVEAMKERIPVQIKVK
                     KINGLGYSKLESAFAVK"
     gene            10113..11297
                     /gene="araJ"
                     /locus_tag="PN977_001465"
     CDS             10113..11297
                     /gene="araJ"
                     /locus_tag="PN977_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414930.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter AraJ"
                     /protein_id="HDI6041513.1"
                     /translation="MKKVILSLALGTFGLGMAEFGIMGVLTELAHNVGISIPAAGHMI
                     SYYALGVVVGAPIIALFSSRYSLKHILLFLVALCVIGNAMFTLSSSYLMLAIGRLVSG
                     FPHGAFFGVGAIVLSKIIKPGKVTAAVAGMVSGMTVANLLGIPLGTYLSQEFSWRYTF
                     LLIAVFNIVVMASVYFWVPDIRDEAKGKLREQFHFLRSPAPWLIFAATMFGNAGVFAW
                     FSYVKPYMMFISGFSETAMTFIMMLVGLGMVLGNVLSGRISGRYSPLRIAAVTDFIIV
                     LALLMLFFCGGMKITSLIFAFICCAGLFALSAPLQILLLQNAKGGELLGAAGGQIAFN
                     LGSAVGAYCGGMMLTLGLAYNYVALPAALLSFAAMSSLLLYGRYKRQQAADSPVLAKP
                     LG"
     gene            complement(11437..12345)
                     /gene="mak"
                     /locus_tag="PN977_001466"
     CDS             complement(11437..12345)
                     /gene="mak"
                     /locus_tag="PN977_001466"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase"
                     /protein_id="HDI6041514.1"
                     /translation="MRIGIDLGGTKTEVIALGDAGEQLYRHRLPTPRDDYRQTIETIA
                     TLVDMAEQATGQRGTVGMGIPGSISPYTGVVKNANSTWLNGQPFDKDLSARLQREVRL
                     ANDANCLAVSEAVDGAAAGAQTVFAVIIGTGCGAGVAFNGRAHIGGNGTAGEWGHNPL
                     PWMDEDELRYREEVPCYCGKQGCIETFISGTGFATDYHRLSGHALKGSEIIRLVEESD
                     PVAELALRRYELRLAKSLAHVVNILDPDVIVLGGGMSNVDRLYQTVPQLIKQFVFGGE
                     CETPVRKAKHGDSSGVRGAAWLWPQE"
     gene            12470..13381
                     /gene="rdgC"
                     /locus_tag="PN977_001467"
     CDS             12470..13381
                     /gene="rdgC"
                     /locus_tag="PN977_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-associated protein RdgC"
                     /protein_id="HDI6041515.1"
                     /translation="MLWFKNLMVYRLSREISLRAEEMEKQLASMAFTPCGSQDMAKMG
                     WVPPMGSHSDALTHVANGQIVICARKEEKILPSPVIKQALEAKIAKLEAEQARKLKKT
                     EKDSLKDEVLHSLLPRAFSRFSQTMMWIDTVNGLIMVDCASAKKAEDTLALLRKSLGS
                     LPVVPLSMENPIELTLTEWVRSGSTAQGFQLLDEAELKSLLEDGGVIRAKKQDLTSEE
                     ITNHIEAGKVVTKLALDWQQRIQFVMCDDGSLKRLKFCDELRDQNEDIDREDFAQRFD
                     ADFILMTGELAALIQNLIEGLGGEAQR"
     gene            complement(13459..13542)
                     /locus_tag="PN977_001468"
     CDS             complement(13459..13542)
                     /locus_tag="PN977_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6041516.1"
                     /translation="MTQRPWSKLQRKTHNIAALKIIARRSE"
     gene            complement(13539..13877)
                     /locus_tag="PN977_001469"
                     /pseudo
     CDS             complement(13539..13877)
                     /locus_tag="PN977_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308469.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2773 domain-containing protein"
     gene            complement(14025..14309)
                     /gene="ppnP"
                     /locus_tag="PN977_001470"
     CDS             complement(14025..14309)
                     /gene="ppnP"
                     /locus_tag="PN977_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine/purine nucleoside phosphorylase"
                     /protein_id="HDI6041517.1"
                     /translation="MLQSNEYFSGKVKSIGFSSSSTGRASVGVMVEGEYTFSTAEPEE
                     MTVISGALNVLLPDATDWQVYEAGSVFNVPGHSEFHLQVAEPTSYLCRYL"
     gene            complement(14381..15058)
                     /locus_tag="PN977_001471"
     CDS             complement(14381..15058)
                     /locus_tag="PN977_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AroM family protein"
                     /protein_id="HDI6041518.1"
                     /translation="MSASLAILTIGIVPMQEVLPLLTEYIDEDNISHHSLLGKLSREE
                     VMAEYAPEAGEDTILTLLNDNQLAHVSRRKVERDLQGVVEVLDNQGYDVILLMSTANI
                     SSMTARNTIFLEPSRILPPLVSSIVEDHQVGVIVPVEELLTVQAQKWQILQKPPVFSL
                     GNPIHDSEQKIIDAGKELLAKGADVIMLDCLGFNQRHRDLLQKQLDVPVLLSNVLIAR
                     LAAELLV"
     gene            complement(15316..15507)
                     /gene="yaiA"
                     /locus_tag="PN977_001472"
     CDS             complement(15316..15507)
                     /gene="yaiA"
                     /locus_tag="PN977_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YaiA"
                     /protein_id="HDI6041519.1"
                     /translation="MPTKPPYPREAYIVTIEKGKPGQTVTWYQLRADHPKPDSLISEH
                     PTAQEAMDAKKRYEDPDKE"
     gene            complement(15557..16081)
                     /gene="aroL"
                     /locus_tag="PN977_001473"
     CDS             complement(15557..16081)
                     /gene="aroL"
                     /locus_tag="PN977_001473"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroL"
                     /protein_id="HDI6041520.1"
                     /translation="MTQPLFLIGPRGCGKTTVGMALADSLNRRFVDTDQWLQSQLNMT
                     VAEIVEREEWAGFRARETAALEAVTAPSTVIATGGGIILTEFNRHFMQNNGIVVYLCA
                     PVSVLVNRLQAAPEEDLRPTLTGKPLSEEVQEVLEERDALYREVAHIIIDATNEPSQV
                     ISEIRSALAQTINC"
     gene            complement(16264..16722)
                     /locus_tag="PN977_001474"
     CDS             complement(16264..16722)
                     /locus_tag="PN977_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HDI6041521.1"
                     /translation="MTIWVDADACPNVIKEILYRAAERMQMPLVLVANQSLRVPPSRF
                     IRTLRVAAGFDVADNEIVRQCEAGDLVITADIPLAAEAIEKGAAALNPRGERYTPATI
                     RERLTMRDFMDTLRASGIQTGGPDSLSQRDRQAFAAELEKWWLEVQRSRG"
     gene            16842..17651
                     /gene="proC"
                     /locus_tag="PN977_001475"
     CDS             16842..17651
                     /gene="proC"
                     /locus_tag="PN977_001475"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HDI6041522.1"
                     /translation="MEKKIGFIGCGNMGKAILGGLIASGQVLPGQIWVYTPSPDKVAA
                     LHDQFGINAAESAQEVAQIADIIFAAVKPGIMIKVLSEITSSLNKDSLVVSIAAGITL
                     DQLARALGHDRKIIRAMPNTPALVNAGMTSVTPNALVTPEDTADVLNIFRCFGEAEVI
                     AEPMIHPVVGVSGSSPAYVFMFIEAMADAAVLGGMPRAQAYKFAAQAVMGSAKMVLET
                     GEHPGALKDMVCSPGGTTIEAVRVLEEKGFRAAVIEAMTKCMEKSEKLSKS"
     gene            complement(17668..18783)
                     /gene="adrA"
                     /locus_tag="PN977_001476"
                     /gene_synonym="dgcC"
     CDS             complement(17668..18783)
                     /gene="adrA"
                     /locus_tag="PN977_001476"
                     /gene_synonym="dgcC"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase AdrA"
                     /protein_id="HDI6041523.1"
                     /translation="MFPKIMNDENFFKKAAAHGEEPPLTPQNEHQRSGLRFARRVRLP
                     RAVGLAGMFLPIASTLVSHPPPGWWWLVLVGWAFVWPHLAWQIASRAVDPLSREIYNL
                     KTDAVLAGMWVGVMGVNVLPSTAMLMIMCLNLMGAGGPRLFVAGLVLMVVSCLVTLEL
                     TGITVSFNSAPLEWWLSLPIIVIYPLLFGWVSYQTATKLAEHKRRLQVMSTRDGMTGV
                     YNRRHWETMLRNEFDNCRRHNRDATLLIIDIDHFKSINDTWGHDVGDEAIVALTRQLQ
                     ITLRGSDVIGRFGGDEFAVIMSGTPAESAITAMLRVHEGLNTLRLPNTPQVTLRISVG
                     VAPLNPQMSHYREWLKSADLALYKAKKAGRNRTEVAA"
     gene            complement(18885..19205)
                     /gene="psiF"
                     /locus_tag="PN977_001477"
     CDS             complement(18885..19205)
                     /gene="psiF"
                     /locus_tag="PN977_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414918.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PsiF"
                     /protein_id="HDI6041524.1"
                     /translation="MKITLLVTLLFGLVFLTTVGAAERTLTPQQQRMTSCNQQATAQA
                     LKGDARKTYMSDCLKNSKSAPGEKSLTPQQQKMRECNNQATQQSLKGDDRNKFMSACL
                     KKAA"
     gene            complement(19324..20739)
                     /gene="phoA"
                     /locus_tag="PN977_001478"
     CDS             complement(19324..20739)
                     /gene="phoA"
                     /locus_tag="PN977_001478"
                     /EC_number="3.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HDI6041525.1"
                     /translation="MKQSTIALALLPLLFSPVTKARTPEMPVLENRAAQGDITAPGGA
                     RRLTGDQTAALRDSLSDKPAKNIILLIGDGMGDSEITAARNYAEGAGGFFKGIDALPL
                     TGQYTHYALNKKTGKPDYVTDSAASATAWSTGVKTYNGALGIDIHEKDHPTILEMAKA
                     AGLATGNVSTAELQDATPAALVAHVTSRKCYGPSATSEKCPGNALEKGGKGSITEQLL
                     NARADVTLGGGAKTFAETATAGEWQGKTLREQAQARGYQLVSDAASLNSVTEANQQKP
                     LLGLFADGNMPVRWLGPKATYHGNIDKPAVTCTPNPQRNDSVPTLAQMTDKAIELLSK
                     NEKGFFLQVEGASIDKQDHAANPCGQIGETVDLDEAVQRALEFAKKDGNTLVIVTADH
                     AHASQIVAPDTKAPGLTQALNTKDGAVMVMSYGNSEEDSQEHTGSQLRIAAYGPHAAN
                     VVGLTDQTDLFYTMKAALGLK"
     gene            complement(20839..21099)
                     /gene="iraP"
                     /locus_tag="PN977_001479"
     CDS             complement(20839..21099)
                     /gene="iraP"
                     /locus_tag="PN977_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraP"
                     /protein_id="HDI6041526.1"
                     /translation="MKNLIAELLFKLAQKEEESKELCAQVEALEIIVTAMLRNMAQND
                     QQRLIDQVEGALYEVKPDASIPDDDTELLRNYVKKLLKHPRQ"
     gene            complement(21281..21484)
                     /locus_tag="PN977_001480"
     CDS             complement(21281..21484)
                     /locus_tag="PN977_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6041527.1"
                     /translation="MLQSSADKACNVLVKKVRYLNDVRKITKMMNGKLIYSWLVWPCS
                     EFFSLNYKLTKRIYFITFIYNKG"
     gene            21562..22656
                     /gene="ddlA"
                     /locus_tag="PN977_001481"
     CDS             21562..22656
                     /gene="ddlA"
                     /locus_tag="PN977_001481"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HDI6041528.1"
                     /translation="MEKLRVGIVFGGKSAEHEVSLQSAKNIVDAIDKSRFDVVLLGID
                     KQGQWHVSDASNYLLNADDPAHIALRPSATSLAQVPGKHEHQLIDAQNGQPLPTVDVI
                     FPIVHGTLGEDGSLQGMLRVANLPFVGSDVLASAACMDKDVTKRLLRDAGLNIAPFIT
                     LTRANRHNISFAEVESKLGLPLFVKPANQGSSVGVSKVTSEEQYAIAVDLAFEFDHKV
                     IVEQGIKGREIECAVLGNDNPQASTCGEIVLTSDFYAYDTKYIDEDGAKVVVPAAIAP
                     EINDKIRAIAVQAYQTLGCAGMARVDVFLTPENEVVINEINTLPGFTNISMYPKLWQA
                     SGLGYTDLITRLIELALERHAADNALKTTM"
     gene            complement(22680..22892)
                     /locus_tag="PN977_001482"
     CDS             complement(22680..22892)
                     /locus_tag="PN977_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414914.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2754 domain-containing protein"
                     /protein_id="HDI6041529.1"
                     /translation="MNLPVKIRRDWHYYAFAIGLIFILNGVVGLLGFEAKGWQTYAVG
                     LVTWVISFWLAGLIIRRRDEETENAQ"
     gene            23152..23460
                     /locus_tag="PN977_001483"
     CDS             23152..23460
                     /locus_tag="PN977_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiY family protein"
                     /protein_id="HDI6041530.1"
                     /translation="MADFTLSKSLFSGKYRNASSTPGNIAYALFVLFCFWAGAQLLNL
                     LVHAPGVYERLMQVQETGRPRVEIGLGVGTIFGLIPFLVGCLIFAVVALWLHWRHRRQ
                     "
     gene            complement(23518..24612)
                     /gene="yaiW"
                     /locus_tag="PN977_001484"
     CDS             complement(23518..24612)
                     /gene="yaiW"
                     /locus_tag="PN977_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="surface-exposed outer membrane lipoprotein YaiW"
                     /protein_id="HDI6041531.1"
                     /translation="MSRVNHLSSLSLLAVLVLAGCSSQAPQPLKKGEKAIDVASVVRQ
                     KMPASVKDRDAWAKDLATTFESQGLAPTLENVCSVLAVAQQESNYQADPAVPGLSKIA
                     WQEIDRRAERMHIPAFLVHTALKIKSPNGKSYSERLDSVRTEKQLSAIFDDLISMVPM
                     GQTLFGSLNPVRTGGPMQVSIAFAEQHTKGYPWKMDGTVRQEVFSRRGGLWFGTYHLL
                     NYPASYSAPIYRFADFNAGWYASRNAAFQNAVSKVSGVKLALDGDLIRYDSKEPGKTE
                     LATRKLAGKLGMSDSEIRRQLEKGDSFSFEETALYKKVYQLAEAKTGKSLPREMLPGI
                     QLESPKITRNLTTAWFAKRVDERRARCMKQ"
     gene            complement(24625..25845)
                     /gene="sbmA"
                     /locus_tag="PN977_001485"
     CDS             complement(24625..25845)
                     /gene="sbmA"
                     /locus_tag="PN977_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide antibiotic transporter SbmA"
                     /protein_id="HDI6041532.1"
                     /translation="MFKSFFPKPGPFFLSAFVWALIAVIFWQAGGGDWVARITGASGQ
                     IPISAARFWSLDFLIFYAYYIVCVGLFALFWFIYSPHRWQYWSILGTALIIFVTWFLV
                     EVGVAVNAWYAPFYDLIQTALSSPHKVTIEQFYREVGVFLGIALIAVVISVLNNFFVS
                     HYVFRWRTAMNEYYMANWQQLRHIEVAAQRVQEDTMRFASTLENMGVSFINAIMTLIA
                     FLPVLVTLSAHVPELPIVGHIPYGLVIAAIVWSLMGTGLLAVVGIKLPGLEFKNQRVE
                     AAYRKELVYGEDDATRATPPTVRELFSAVRKNYFRLYFHYMYFNIARILYLQVDNVFG
                     LFLLFPSIVAGTITLGLMTQITNVFGQVRGAFQYLINSWTTLVELMSIYKRLRSFEHE
                     LDGDKIQEVTHTLS"
     gene            26197..27354
                     /gene="ampH"
                     /locus_tag="PN977_001486"
     CDS             26197..27354
                     /gene="ampH"
                     /locus_tag="PN977_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine-
                     carboxypeptidase/endopeptidase AmpH"
                     /protein_id="HDI6041533.1"
                     /translation="MKRSLLFSAVLCAASLTSVHAAQPITEPEFASDIVDRYADHIFY
                     GSGATGMALVVIDGNQRVFRSYGETRPGNNVRPQLDSVVRIASLTKLMTSEMLVKLLD
                     QGTVKLNDPLSKYAPPGARVPTYNGTPITLVNLATHTSALPREQPGGAAHRPVFVWPT
                     REQRWKYLSTAKLKAAPGSQAAYSNLAFDLLADALANASGKPYTQLFEEQITRPLGMK
                     DTTYTPSPDQCRRLMVAERGASPCNNTLAAIGSGGVYSTPGDMMRWMQQYLSSDFYQR
                     SNQADRMQTLIYQRAQFTKVIGMDVPGKADALGLGWVYMAPKEGRPGIIQKTGGGGGF
                     ITYMAMIPQKNIGAFVVVTRSPLTRFKNMSDGINDLVTELSGNKPLVIPAS"
     gene            complement(27355..27978)
                     /gene="iprA"
                     /locus_tag="PN977_001487"
     CDS             complement(27355..27978)
                     /gene="iprA"
                     /locus_tag="PN977_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogen peroxide resistance inhibitor IprA"
                     /protein_id="HDI6041534.1"
                     /translation="MLSVVKPLQEFGKLDKCLSRYGTRFEFNNEKQVIFSSDVNSEDT
                     FVILEGVISLRREENVLIGITQAPYIMGLADGLMKNDIPYKLISEGNCTGYHLPAKQT
                     ITLIEQNQLWRDAFYWLAWQNRILELRDVQLIGHNSYEQIRATLLSMIDWNEELRSRI
                     GVMNYIHQRTRISRSVVAEVLAALRKGGYIEMNKGKLVAINRLPSEY"
     gene            complement(28064..30973)
                     /locus_tag="PN977_001488"
     CDS             complement(28064..30973)
                     /locus_tag="PN977_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HDI6041535.1"
                     /translation="MHSWKKKLVVSQLALACTLAITSQANATTYDTWTYVDNAATGLN
                     WTNMDNPGTVDGNYVNYSGFVYYNNTNGDFDQSFNGDTVNGTISTYYLNHDYTDGTNG
                     NANQLDIANSVIHGSITSMLPGGDLWHNNAYDQSYGYIQAGDTVIDTTWYDGDVFTLN
                     IANSTIDDDYEALYFTDYYKDGDVTKSTNGTFDVSEGVAVNLNVESNINISNNSRVAG
                     IALSQGSTYNNTYTTESHTWDNNINVSNSTVTTGSNTVLESSGYGHFGNSGEPSDYAG
                     PGDVALSFTDSTSDYAMKNNVYFSNSTLVGDVAFTSTWNANFDPSGHDSNGDGVKDTN
                     AGWVDDSLNVDELNITLDNGSKWVGQATFNAETISPDTMYDVATNSLTPGGTAEANGW
                     NRIIDNKVFQSGVFNVALNNGSEWDTTGRSVVDTLTVNNASQVNVSESKLTSDTIDLT
                     NGSSLNIGEDGYVDTDHLTINSYSTVALTESTGWGADYNLYANTITVTNGGVLDVNVD
                     QFDTEAFRTDKLELTSGNIADNNGNVVSGVFDIHSSDYVLNADLVNDRTWDTSKSNYG
                     YGIVAMNSDGHLTINGNGDMNNGDELDNSSVDNVVAATGNYKVRIDNATGAGAIADYK
                     DKEIIYVNDVNSNATFSAANKADLGAYTYQAEQRGNTVVLQQMELTDYANMALSIPSA
                     NTNIWNLEQDTVGTRLTNSRHGLADNGGAWVSYFGGNFNGDNGTINYDQDVNGIMVGV
                     DTKIDGNNAKWIVGAAAGFAKGDMNDRSGQVDQDSQTAYIYSSAHFANNVFVDGSLSY
                     SHFNNDLSATMSNGTYVDGSTNSDAWGFGLKAGYDFKLGDAGYVTPYGSVSGLFQSGD
                     DYQLSNNMKVDGQSYDSMRYELGVDAGYTFTYSEDQALTPYFKLAYVYDDSNNHNDVN
                     GDSIDNGTEGSAVRVGLGTQFSFTKNFSAYTDANYLGGGDVDQDWSANVGVKYTW"
     gene            31499..32473
                     /gene="hemB"
                     /locus_tag="PN977_001489"
     CDS             31499..32473
                     /gene="hemB"
                     /locus_tag="PN977_001489"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HDI6041536.1"
                     /translation="MTDLIQRPRRLRKSPALRAMFEETTLSLNDLVLPIFVEEEIDDY
                     KAVEAMPGVMRIPEKHLAREIERIANAGIRSVMTFGISHHTDETGSDAWREDGLVARM
                     SRICKQTVPEMIVMSDTCFCEYTSHGHCGVLCEHGVDNDATLENLGKQAVVAAAAGAD
                     FIAPSAAMDGQVQAIRQALDAAGFKDTAIMSYSTKFASSFYGPFREAAGSALKGDRKS
                     YQMNPMNRREAIHESLLDEAQGADCLMVKPAGAYLDIVRELRERTELPIGAYQVSGEY
                     AMIKFAALAGAIDEEKVVLESLGSIKRAGADLIFSYFALDLAEKKILR"
     gene            complement(32513..33364)
                     /gene="tauD"
                     /locus_tag="PN977_001490"
     CDS             complement(32513..33364)
                     /gene="tauD"
                     /locus_tag="PN977_001490"
                     /EC_number="1.14.11.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine dioxygenase"
                     /protein_id="HDI6041537.1"
                     /translation="MSERLSITPLGPYIGAQISGADLTRPLSDNQFEQLYHAVLRHQV
                     VFLRDQAITPQQQRALAQRFGELHIHPVYPHAEGVDEIIVLDTHNDNPPDNDNWHTDV
                     TFIETPPAGAILAAKELPSTGGDTLWTSGIAAYEALSVPFRQLLSGLRAEHDFRKSFP
                     EYKYRKTEEEHQRWREAVAKNPPLLHPVVRTHPVSGKQALFVNEGFTTRIVDVSEKES
                     EALLSFLFAHITKPEFQVRWRWQPNDIAIWDNRVTQHYANADYLPQRRIMHRATILGD
                     KPFYRAG"
     gene            complement(33361..34188)
                     /gene="tauC"
                     /locus_tag="PN977_001491"
     CDS             complement(33361..34188)
                     /gene="tauC"
                     /locus_tag="PN977_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter permease TauC"
                     /protein_id="HDI6041538.1"
                     /translation="MSVLINEKLHSHRLKWRWPLSRQVTLSIGTLAVLLTVWWAVAAL
                     QLISPLFLPPPQQVLAKLLTIAGPQGFMDATLWQHLAASLTRILLALLAAVLIGIPVG
                     IAMGLSPTVRGILDPIIELYRPVPPLAYLPLMVIWFGIGETSKILLIYLAIFAPVAMS
                     ALAGVKSAQQVRIRAAQSLGASRAQVLWFVILPGALPEILTGLRIGLGVGWSTLVAAE
                     LIAATRGLGFMVQSAGEFLATDVVLAGIAVIAIIAFLLELGLRALQRRLTPWHGEVQ"
     gene            complement(34185..34952)
                     /gene="tauB"
                     /locus_tag="PN977_001492"
     CDS             complement(34185..34952)
                     /gene="tauB"
                     /locus_tag="PN977_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter ATP-binding subunit"
                     /protein_id="HDI6041539.1"
                     /translation="MLQISHLYADYGGKPALEDINLTLESGELLVVLGPSGCGKTTLL
                     NLIAGFVPYQHGSIQLAGKRIEGPGAERGVVFQNEGLLPWRNVQDNVAFGLQLAGVEK
                     MQRLEIAHQMLKKVGLEGAEKRYIWQLSGGQRQRVGITRALAANPQLLLLDEPFGALD
                     AFTRDQMQTLLLKLWQETGKQVLLITHDIEEAVFMATELVLLSSGPGRVLERLPLNFA
                     RRFVAGEPSRSIKSDPQFIAMREYVLSRVFEQREAFS"
     gene            complement(34966..35928)
                     /gene="tauA"
                     /locus_tag="PN977_001493"
     CDS             complement(34966..35928)
                     /gene="tauA"
                     /locus_tag="PN977_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter substrate-binding
                     protein"
                     /protein_id="HDI6041540.1"
                     /translation="MAISSRNTLLAALAFIAFQAQAVNVTVAYQTSAEPAKVAQADNT
                     FAKESGATVDWRKFDSGASIVRALASGDVQIGNLGSSPLAVATSQQVPIEVFLLASKL
                     GNSEALVVKKTISKPEDLIGKRIAVPFISTTHYSLLAALKHWGIKPGQVEIVNLQPPA
                     IIAAWQRGDIDGAYVWAPAVNALEKDGKVLTDSEQVGQWGAPTLDVWVVRKDFAEKHP
                     EVVKAFAKSAIDAQQPYIANPDAWLKQPENISKLARLSGVPEGDVPGLVKGNTYLTPQ
                     QQTAELTGPVNKAIIDTAQFLKDQGKVPAVANDYSQYVTSRFVQ"
     gene            36544..37215
                     /locus_tag="PN977_001494"
     CDS             36544..37215
                     /locus_tag="PN977_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016241930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HDI6041541.1"
                     /translation="MDKVLDSALLSSANKRKGILAIGAHPDDIELGCGASLARLAQKG
                     IYIAAVVMTTGNSGTDGIIDRHEESRNALKILGCHQTIHLNFADTRAHLQLNDMISAL
                     EDIIKNQIPSDVEIMRVYTMHDADRHQDHLAVYQASMVACRTIPQILGYETPSTWLSF
                     MPQVFESVKEEYFTVKLAALKKHKSQERRDYMRHDRLRAVAQFRGQQVNSDLGEGFVI
                     HKMIL"
     gene            37225..38421
                     /locus_tag="PN977_001495"
     CDS             37225..38421
                     /locus_tag="PN977_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HDI6041542.1"
                     /translation="MKTWIFICMAVAILLWFLSTLRRKPSQKKGCIDAIIPAYNEGPC
                     LAQSLDNLLRNPYFCRVICVNDGSTDNTEAVMAEVKRKWGDRFIAVTQKNTGKGGALM
                     NGLNYATCDQVFLSDADTYVPPDQDGMGYMLAEIERGADAVGGIPSTALKGAGLLPHI
                     RATVKLPMIVMKRTLQQLLGGAPFIISGACGMFRTDVLRKFGFSDRTKVEDLDLTWTL
                     VANGYRIRQANRCIVYPQECNSPREEWRRWRRWIVGYAVCMRLHKRLLFSRFGIFSIF
                     PMLLVVLYGVGIYLTTWFNEFITTGPHSVVLAMFPLIWVGVVCVIGAFSAWFHRCWLL
                     VPLAPLSVVYVLLAYAIWIIYGLIAFFTGREPQRDKPTRYSALVEASTAYSQPSVTGT
                     EKLSEA"
     gene            38399..38980
                     /locus_tag="PN977_001496"
     CDS             38399..38980
                     /locus_tag="PN977_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012602184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HDI6041543.1"
                     /translation="MKNFLKLNVKEKNIQIADQVIIDETAGEVVIGANTRICHGAVIQ
                     GPVVIGANCLIGNYAFIRPGTIISNGVKIGFATEIKNAVIEAEATIGPQCFIADSVVA
                     NQAYLGAQVRTSNHRLDEQPVSVRTPEGIIATGCDKLGCYIGQRSRLGVQVIILPGRI
                     ISPNTQLGPRVIVERNLPTGTYSLRQELIRTGD"
     gene            38982..39755
                     /locus_tag="PN977_001497"
     CDS             38982..39755
                     /locus_tag="PN977_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiO family outer membrane beta-barrel protein"
                     /protein_id="HDI6041544.1"
                     /translation="MIKRTLLAAAIFSTLPAYAGLTSITAGYDFTDYSGDHGNRNLAY
                     AELVAKVENATLLFNLSQGRRDYETEHFNATRGQGAVWYKWNNWLTTRTGIAFADNTP
                     VFARQDFRQDINLALLPKTLFTTGYRYTKYYDDVEVDAWQGGVSLYTGPVISSYRYTH
                     YDSSDAGGSYSNMISVRLNDPRGTGYTQLWLSRGTGAYTYDWTPETRYGSMKSVSLQR
                     IQPLTEQLNLGLTAGKVWYDTPTDDYNGLQLAAHLTWKF"
     gene            39940..40215
                     /gene="frmR"
                     /locus_tag="PN977_001498"
     CDS             39940..40215
                     /gene="frmR"
                     /locus_tag="PN977_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414891.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formaldehyde-responsive transcriptional
                     repressor FrmR"
                     /protein_id="HDI6041545.1"
                     /translation="MPSTPEEKKKVLTRVRRIRGQIDALERSLEGDAECRAILQQIAA
                     VRGAANGLMAEVLESHIRETFDRNDCYSREVSQSVDDTIELVRAYLK"
     gene            40250..41359
                     /gene="frmA"
                     /locus_tag="PN977_001499"
     CDS             40250..41359
                     /gene="frmA"
                     /locus_tag="PN977_001499"
                     /EC_number="1.1.1.284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-(hydroxymethyl)glutathione dehydrogenase"
                     /protein_id="HDI6041546.1"
                     /translation="MKSRAAVAFAPGKPLEIVEIDVAPPKKGEVLIKVTHTGVCHTDA
                     FTLSGDDPEGVFPVVLGHEGAGVVVEVGEGVTSVKPGDHVIPLYTAECGECEFCRSGK
                     TNLCVAVRETQGKGLMPDGTTRFSYNGQPLYHYMGCSTFSEYTVVAEVSLAKINPEAN
                     HEHVCLLGCGVTTGIGAVHNTAKVQPDDSVAVFGLGAIGLAVVQGARQAKAGRIIAID
                     TNPKKFDLARRFGATDCINPNDYDKPIKDVLLDINKWGIDHTFECIGNVNVMRAALES
                     AHRGWGQSVIIGVAGSGQEISTRPFQLVTGRVWKGSAFGGVKGRSQLPGMVEDAMKGD
                     IDLEPFVTHTMSLDEINDAFDLMHEGKSIRTVIRY"
     gene            41451..42284
                     /gene="fghA"
                     /locus_tag="PN977_001500"
     CDS             41451..42284
                     /gene="fghA"
                     /locus_tag="PN977_001500"
                     /EC_number="3.1.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HDI6041547.1"
                     /translation="MELIEKHASFGGWQNVYRHYSQSLKCEMNVGVYLPPKAGNEKLP
                     VLYWLSGLTCNEQNFITKSGMQRYAAEHNIIVVAPDTSPRGSHVADADRYDLGQGAGF
                     YLNATQAPWNEHYKMYDYIHNELPDLVMQHFPATTRKSISGHSMGGLGALVLALRNPD
                     EYVSVSAFSPIVSPSQVPWGQQAFAAYLGENKEAWLDYDPVSLISQGQRVAEIMVDQG
                     LSDDFYAEQLRTPNLEKICQEMNIKTLIRYHEGYDHSYYFVSSFIGEHIAYHANKLNM
                     R"
BASE COUNT        10724 a        10979 c        10364 g        10238 t
ORIGIN      
        1 tcagccacag tgtaataagc agttgatctt tgctttgttt aacaaatggg ggcaccggca
       61 ggtgccatgc atttaacatc ataattcccc tgtgatgaaa atctgatcac cttgccatag
      121 cctaatgcag gatcactcat catctgaagg atatttgggg gaggaggacg ggggcggata
      181 actttcggaa tagccggatc aggatttcct cctgaaccgg cttacagaac gcaaggatta
      241 ctggttttca gccagctgac gatcgtggcg gcgtttaaac atccagccaa tcaacagcac
      301 aacaatccac gcgaaaccaa cgtacagcga gatacgcgta tccgggtgat aaccaatcaa
      361 cccgataata aagagcagga aaatcaaacc gccgatggtc gttgctaccc cacccggcac
      421 tttaaatttc agcgccttaa cttcttctgg aggcaaacgg cgacggaagg cgatttgcga
      481 cagcaggatc ataatccaca cccacaccgt ggcgaaggtt gccagcgaag cgatcaccag
      541 gaagacgttt tccggcatga tgtagttcag atacaccgca aatagcagcg cggtagtcat
      601 caccagcacc gtaacccacg gaataccgcg acgcgaggtt ttgctgaaaa tttttggcgc
      661 gctgccctgc tctgccatac cgtggagcat acggcctacg ccaaatacgt cactgttaat
      721 tgccgacagc gaagcggtca gcacaacaaa gttaagaatg ctggcggcaa aggtaatgcc
      781 catatgctgg aacgtcagca cgaacgggct accggcagtg ccaacctgat tccacgggta
      841 gatagacatt atgacgaaca gcgtaccgac gtagaacacc agaatacgca tcggcacgga
      901 gttaatcgca cgcggaatcg atttctcagg atctttcgct tcaccggcgg taatcccgat
      961 aatttcgatc ccaccgtaag caaacatcac catttgcaac gacatcacca taccaagcca
     1021 gccgttactg aagaagccgc cgttgctcca caggttatga ataccggtcg gttgcccgcc
     1081 gttgccaatt ccccagatga tgatgccgaa accggcgaca atcatgatga tgatagtggc
     1141 gactttaaag aacgagaacc agaactccag ctcaccgaat accttcacgc tcatcaggtt
     1201 tacggcgcaa atgatcagca ccacgctcag tacccaaatc cagtgcggca ccgtcgggaa
     1261 ccagaccccc atatagatac caaaagcggt cacatcggcg atggcgacaa ttaggatttc
     1321 aaagcagtag gtccagccgg taatgtaacc tgccagcggt ccgaggtttt cctgcgcata
     1381 acgcgagaaa gagctggcgg ccgggttatg taccgacatt tcccccagcg cgcgcataat
     1441 gatatacgcc gcgataccac cgataatata ggccaacaac acgcttggac cggccatttt
     1501 gatggcatct gccgaaccgt aaaacagccc ggtgccaatt gctgaaccca gtgccataaa
     1561 gcgtatgtgt cgggtactta gcccacgctt tagcttgttc ttactttcca tcaattccta
     1621 acccatcaac acaataaaaa accacggggc atcgcagccc cgtggttaaa acaaatgttc
     1681 agtgatttag tgagcgctgg aggtcacctg acgacctgcc gcacgatccc agataatggc
     1741 cagaaccacc atcaccactg ttggcattaa ccacgccaga ccttgttcgg ccagcggtaa
     1801 acgctgcgcc caggacggta agatatcgct gaatgcagat gccttgatcc cgtcgagaat
     1861 accaaaaagc aggctgataa acatcggcgg agcaatcacg cgggacgaat tatgccacca
     1921 tgagcgtgta aaacttaata caaccagtgc gatacacggc ggataaatgg cggtcaacac
     1981 cggtacagag atctgaatca gctggctcaa gccgaggtta gacaccacca tcgagaagcc
     2041 gccgaggata aacaccagcg tacgataaga gagcggtacg tactgggcga agaattctgc
     2101 acaagcacag gtcaggccaa ccgccgtgac caggcaggcg atgaagatta acgccgccag
     2161 caggaagcta ccgccgccgc caaaggtatg ctgaacgtaa gcatgcagga tcgccgcacc
     2221 gtttgcagac tgatcgacca gcgacgcgct gtctgaaccc agacggaaca gcgccagata
     2281 cagcagagtc agaccaacac ccgccatcag gccagcccag acggtataac gggtcagcag
     2341 acgcgcttcg gtaacgccac gagaacgcgc cgcgttaaca ataacgatac caaacaccat
     2401 tgcgcccagc gtatccatgg tcagatagcc gttgacgaag ccgttagaaa acgcagcgtt
     2461 ttgataagcc tcagtcgccg tgctgataga acccgccggc cagataattg cggcaacaga
     2521 caggatgacc agcgcgataa ttttcagcgg cgcaaggaag ttgcccacgg tatccagcag
     2581 cttgcccgga tagagcgaaa ccagaataac gatagcgaaa taaaccaggc tgtagataaa
     2641 cagcggcaac gcggaatcac ccgtcagcgg cgcaatgccc acttcaaaag aaacggtagc
     2701 tgtacgcggc gtagcgaaaa gcggccccac cgccaggtaa cacaccgttg ccagcagtac
     2761 gccagcgact ttaccgattg gcgtgctgag gctgtcaaca ccgccgccaa ctttcgccag
     2821 cgccaccacc gttaacaccg gtaggccaac ggcagtaatg aggaaaccga atgccgcagt
     2881 ccagacgtgt tcgcctgcct gcaagccgac cattggaggg aaaataatgt tacctgcgcc
     2941 gacgaacaac gcaaatgtca taaagcccag agcgatgata tcgcgcgatc ttaattgatg
     3001 ggtcataaat catactgcct gtggatgtgg tgttgtaacg ttaaattttt gtgccaatcc
     3061 ctggggatga tacagcggta tatttaaaca attttagcgg gaaatactcc caaccaggcg
     3121 cggcgaagaa tagtttttcg atttaccgcg taaatgcagt cagtatgaca gcacctgaag
     3181 cgcaatttaa acgcttataa cgttttaaag caagaaggga tctaaaaacc agaggtatat
     3241 agcgataaga aatcaccaac cagtataaaa cactatttca atgaaaaaga catggctaat
     3301 catgcgaaca aaaaatcacc aaccattgta aaatctaacg cccgtatttc gactgcaaag
     3361 aaaataagcc agcatcggct ggcttatggc aataaaagat gacaaaggcg aattaatcgc
     3421 tgtttttggc aattaaacgt tccgggataa caaaactgaa acgtgtacct tttcctactg
     3481 tactctcaat attcaggcga ctttcgtgat gattcaccgc atgtttcacg atcgctaacc
     3541 ctaatccgct accaccggtt tgccgggaac gcgctttatc aacgcgataa aaacgctcgg
     3601 tcaggcgcgg aatatgctcc ggtgcaatgc ccggtccgtt atcttcaacg ctaaattcgg
     3661 caccatgcgg cactcgctgc cagcgtacgg tgatatgcgt gccttccggc gtatgattca
     3721 cggcgttata gaccaggttc gaaatcgcac tgcgtagctg atcttcgctg cctgacacct
     3781 tgaggccgtt atctatctca aaggtaaatg tctgtttttt ctgactcaga gtctgagcct
     3841 cgcgctcaac aacgcgcagc atcatcggca catcaacctt ttcattgagc aactgcgtcg
     3901 gtgcggcttc tattttcgac agcgtcagca attgcttcac cagcccttcc atccgctgtg
     3961 tctgctcgcg catggtgtgc aacgcttttt cacgtaccgc gccttccagc ggttgctcat
     4021 ccatcatctc caggtaaccc tgtaacacgg tcaatggcgt acgtaactca tggctcacgt
     4081 tggcaaaaaa gttacgccgc gccccttcca gttgatgcat ttgcgtcaca tcacgcgcca
     4141 ccatcagcaa ctgtttgtgg gtataaggca tgacgcgaat ttccagatgc cgcccggtat
     4201 tgagcaccag attgagcggg cgagaaaaat cacgcgtttt cagatattgc gtaaactccg
     4261 gataacgaag cagattaagg atgttctgcc cgttatcttc cggccagcgc aaaccaagaa
     4321 tttgttgcgc cagaccatta caccagaaaa taccgccctc ttccgtggtc agcaccaccg
     4381 catcaggcag cgactccgcg ccgctacgaa agcgtttaat cagattgccc agttcgcggc
     4441 ggcgtttttt atttcgcaac tgcatctggt gtaatccgta tagcagcggt tcccagctac
     4501 cacgccccgg tggcggggtc atactacgat ccacccacag ccaccatgaa aggcgcaata
     4561 aattccagaa atgccagata agcagtcccg ttaccgatgc cagcaaaaac cagggcaggt
     4621 aaccaaaaaa tgcacccagg atgaaagccg ggaggcagca aagtagcagc tccagcacca
     4681 gccttttcca cgacagccgt tccagcacgt aagatactcc agttaagaat tcataagccc
     4741 tgctctgcgt ccgatgagca aggcgttaaa agcgggttga aaaacgatat cctgtaccgc
     4801 gcacggtctg caccatgcgg tcatgcccgc cgggctccag tgctttacgc aggcgacgaa
     4861 tgtggacatc gaccgtacga tcttccacat acacgttggt tccccagacg tggtttaaca
     4921 gctgctcgcg gctgtacacg cgctcaggat gcgtcataaa aaagtgcagc agtttaaatt
     4981 ctgtcggccc catctccagc ggttcttcgc ccgccatcac ccggtgagat gtcgggtcga
     5041 gactgagccc ctgcatctca atcacctctt ccaccgccat tggcgaaata cggcgcatta
     5101 ccgctttgat tcgcgccacc agctccttcg gcgaaaacgg cttggtgata tagtcatccg
     5161 caccggtttc aaggccgcgc acgcgatctt cttcttcccc tctggcggtc aacatcacca
     5221 ctggaatatc ccgggtcatc gactcgcgct tgaggtgttt gatgaactgg ataccggagc
     5281 cgccaggtaa catccagtca aggagaatta aatccggcca gggttcattc agttgattca
     5341 cagcactgtc ataatcttcc gcttcgaccg gctgaaagcc attttgttcg agcacgaagc
     5401 agaccatttc gcgaattggt gcttcatctt ctacgaccag aatacgtctc gccatgattt
     5461 gccctgttgt aataaatagg ttgcgatcat taatgcgacg tcattatgcg tcagatttat
     5521 gacagattta tgaaaagctc gtcgcacata tcttcaggtt attgatttcc gtggcgcaga
     5581 aaaaagcaaa tggcacatct gtttgggtat aatcgcgccc atgctttttc gccagggaac
     5641 cgttatgcgc atccttcaca cctcagactg gcatctcggc cagaacttct acagtaaaag
     5701 ccgcgaagct gaacatcagg cttttcttga ctggctgctg gagacagcac aaacccatca
     5761 ggtggatgcg attattgttg ctggtgatgt tttcgatacc ggctcaccgc ccagttacgc
     5821 ccgcacgtta tacaaccgtt ttgttgtcaa tttacagcaa actggctgtc atctggtggt
     5881 actggcagga aaccatgact cggtcgccac gctgaatgaa tcgcgcgata tcatggcgtt
     5941 cctcaatact accgtggtcg ccagcgccgg acatgcgccg caaatcttgc ctcgtcgcga
     6001 cgggacgcca ggcgcagtgc tgtgccccat acctttttta cgcccgcgtg acattattac
     6061 cagccaggcg gggcttaacg gtattgaaaa acagcagcat ttgctggcgg ctattactga
     6121 ttattatcaa caacattatg ccgatgcctg caaactgcgc ggcgatcagc ctctgcccat
     6181 catcgccacg ggacatttaa cgaccgtggg tgccagtaaa agtgacgccg tgcgtgacat
     6241 ttatattggc acgctggacg cgtttccggc acaaaacttt ccaccagccg actacatcgc
     6301 gctcgggcat attcaccgcg cgcagattat tggcggcatg gaacatgttc gctattgcgg
     6361 ttcccccata ccactgagtt ttgatgaatg cggtaagagt aaatatgtcc atctggtgac
     6421 attttcaaac ggcaaattag agagcgtgga aaacctgaac gtaccggtaa cgcaacccat
     6481 ggcggtgctg aaaggcgatc tggcgtcgat taccgaacag ctggaacagt ggcgcgatgt
     6541 atcacaggag ccacctgtct ggctggatat cgaaatcact actgatgagt atctgcatga
     6601 tattcagcgc aaaatccagg cattaaccga atcattgccc gttgaagtat tgctggtacg
     6661 ccggagtcgt gaacagcgcg agcgtgtgtt agccagccaa cagcgtgaaa ccctcagcga
     6721 actcagcgtc gaagaggtgt tcaatcgccg tctggcactg gaagaactgg atgaatcaca
     6781 gcagcaacgt ctgcagcatc ttttcgccac gacattgcat agcctcgccg gagaacacga
     6841 agcatgaaaa ttctcagcct gcgcctgaaa aacctgaact cattaaaagg cgaatggaag
     6901 attgatttca cccgcgagcc gttcgccagc aacgggctgt ttgctattac cggcccaacc
     6961 ggtgcgggga aaaccaccct gctggacgcc atttgtctgg cgctgtatca cgaaaccccg
     7021 cgcctctcta acgtttcaca atcgcaaaat gatctcatga ctcgcgatac cgccgaatgt
     7081 ctggcggaag tggagtttga agtcaaaggt gaagcgtatc gcgcgttctg gagccagaat
     7141 cgggcgcgta accagccgga cggtaatttg caggtgccac gcgtagagct ggcgcgctgc
     7201 gcagacggca aaattctcgc cgacaaagtg aaagataagc tggaactgac ggcgacgtta
     7261 accggactgg attacgggcg cttcacccgt tcgatgctgc tttcgcaggg gcaatttgct
     7321 gccttcctga atgccaaacc caaagaacgc gcggaattgc tcgaggagtt aaccggcact
     7381 gaaatctacg ggaaaatctc tgcgatggtt tttgagcagc acaaatcggc ccgcacagag
     7441 ctggagaagc tgcaagcgca ggccagcggc gtcgcgttgc tcacgccaga acaagtgcaa
     7501 tcgctgacag cgagtttgca ggtacttact gacgaagaaa aacagttaat taccgcgcag
     7561 cagcaagaac aacaatcgct aaactggtta acgcgtctgg acgaattgca gcaagaaggc
     7621 agccgccgtc aacaggcctt gcaacaggca ttagccgaag aagaaaaagc gcaacctcaa
     7681 ctggcggcgc tcagtctggc acaaccggca cgaaatcttc gtccacactg ggaacgcatc
     7741 gcagaacaca gcgcggcgct ggcgcatact cgccagcaga ttgaagaagt aaatactcgc
     7801 ttacagagca caatggcgct tcgggcgagc attcgccacc acgcggcgaa gcagtcagca
     7861 gaattacagc agcagcaaca aagtctgaat gcctggttac aggaacacga ccgcttccgt
     7921 cagtggaaca acgaactggc gggttggcgt gcgcagttct cccaacaaac cagcgatcgc
     7981 gagcatctgc ggcaatggca gcaacagtta acccatgcag agcaaaaact taatgcgctt
     8041 gcggcgatta cgttgacgtt aaccgccgat gaagttgcta gcgccctggc gcaacatgcc
     8101 gagcaacgcc cactgcgtca gcgcctggtc gcgctgcatg gacagattgt tccccaacaa
     8161 aaacgtctgg cgcagttaca ggtcgctatc cagaatgtca ctctggaaca gacgcaacgt
     8221 aacgccgcac ttaacgaaat gcgccagcgt tataaagaaa agatgcagca acttgccgat
     8281 gtcaaaacca tttgcgagca ggaagcacgc atcaaaacgc tggaagccca gcgcgcgcag
     8341 ttacaggcgg gtcagccttg tccactttgt ggttccacca gccacccggc ggtcgaggcg
     8401 tatcaggcgc tggagcctgg cgttaatcag gcccggctat taacgctgga aaaagaagtg
     8461 aaaaagctcg gcgaagaagg tgcggcgcta cgtgggcaac tggatgcatt aacgaagcag
     8521 cttcagcgcg atgaaaacga agcgcaaagc ctccgacaag atgagcaagc acttactcaa
     8581 caatggcaag ccgtcacggc cagcctcaat atcaccctgc agccacagga cgatattcaa
     8641 ccgtggctag atgcacaaga tgagcacgaa cgccagctgc ggttactcag ccaacggcat
     8701 gaattacaag ggcagattgc cgcgcataat cagcaaatta tccagtatca acagcaaatt
     8761 gaacaacgcc agcagcaact cttaacggca ttggcgggtt atgcgctgac attgccacag
     8821 gaagatgaag aagagagctg gttggcggcg cgtcagcaag aagcgcagcg ctggcagcaa
     8881 cgccagaacg aattaaccgc gctgcaaaac cgtattcagc aactgacgcc gattctggaa
     8941 acgttgccgc aaagtgatga actcccgcac tgcgaagaaa ctgtggtact ggaaaactgg
     9001 cggcaggtac atgaacaatg tctcgcacta cacagccagc aacagacgtt acagcaacag
     9061 gacgttctgg cggcgcaaag tctgcaaaaa gcccaggcgc agtttgacac cgcgctacag
     9121 gcgagcgtct ttgacgatca acaggcgttc cttgcggcgc taatggatga acaaacacta
     9181 acgcagctgg aacagctcaa gcagaatctg gaaaaccagc gccgtcaggc gcaaacgctg
     9241 gtcactcaga cagcagaagc gctggcacag catcagcaac accgacctga cggcctggat
     9301 ctcagtgtga cggtggagca gattcagcaa gagttagcgc aaactcagca aaagttgcgt
     9361 gaaaatacca ccagtcaggg cgagattcgc cagcagctga agcaggatgc agacaaccgt
     9421 cagcaacaac aaaccttaat gcagcaaatt gctcaaatga cgcagcaggt tgaggactgg
     9481 ggatatctga attcgctaat aggttccaaa gagggcgata aattccgcaa gtttgcccag
     9541 gggctgacgc tggataattt agtccatctc gctaatcagc aacttacccg gctgcacggg
     9601 cgctatctgt tacagcgcaa agccagcgag gcgctggaag tcgaggttgt tgatacctgg
     9661 caggcagatg cggtacgcga tacccgtacc ctttccggcg gcgaaagttt cctcgtcagt
     9721 ctggcgctgg cgctggcgct ttcggatctg gtcagccata aaacgcgtat tgactcgctg
     9781 ttccttgatg aaggttttgg cacgctggat agcgaaacgc tggataccgc ccttgatgcg
     9841 ctggatgccc tgaatgccag tggcaaaacc atcggtgtga ttagccacgt tgaagcgatg
     9901 aaagagcgta ttccggtgca gatcaaagtg aaaaagatca acggcctggg ctacagcaaa
     9961 ctggaaagtg cgtttgcagt gaaataacta ttcagcagga taatgaatac agaggggcga
    10021 attatctctt ggcctcgctg gtcgttatcc tgcaagctat cactttattg gctacggtga
    10081 ttagtagccg ttctggtggt tgtgatggtg gtatgaaaaa agtcatttta tctttggctc
    10141 tgggcacgtt tggtttgggg atggccgaat ttggcattat gggcgtgctc acggagctgg
    10201 cgcataacgt aggaatttcg attcctgctg ccgggcatat gatctcgtat tatgcgctgg
    10261 gggtggtggt cggtgcgcca atcatcgcac tcttttccag ccgctactca ctcaaacata
    10321 tcttattgtt tctggtggcg ttgtgcgtca ttggcaacgc catgttcacg ctctcttcgt
    10381 cttacctgat gctcgccatt ggtcggctgg tatccggctt tccgcatggc gcattttttg
    10441 gcgtcggcgc gatcgtgtta tcaaaaatta tcaaacccgg aaaagtcacc gccgccgtgg
    10501 cggggatggt ttccgggatg acagtcgcca atttgctggg cattccgctg ggaacgtatt
    10561 taagtcagga atttagctgg cgttacacct ttttattgat cgctgttttt aatattgtgg
    10621 tgatggcatc ggtctatttt tgggtgccgg atattcgcga cgaagcgaaa ggaaagctgc
    10681 gcgaacaatt tcacttttta cgcagcccgg ccccgtggtt aattttcgcc gccaccatgt
    10741 ttggcaacgc aggtgtattt gcctggttca gctacgtaaa gccatacatg atgtttattt
    10801 ccggtttttc ggaaacggcg atgaccttta ttatgatgtt agtggggcta gggatggtgc
    10861 tggggaatgt gctaagtggc cgaatttcag gacgttattc accactgcgc attgcagcag
    10921 tgactgactt tatcattgta ctggcactgc tgatgctctt tttctgcggc ggcatgaaaa
    10981 taacgtcgct tatttttgct tttatttgtt gcgcgggatt atttgccctt tcagcacctc
    11041 tgcaaatatt gttactgcaa aacgccaaag gcggagagtt attaggtgcc gcaggtgggc
    11101 aaatagcgtt taacctcggt agcgccgtcg gcgcatattg cggtggtatg atgctgacgc
    11161 tggggctggc atataattac gtggcgctgc ctgccgccct gctttcgttt gctgcgatgt
    11221 cgtcgttgct gctgtatggt cgctataagc gccagcaagc ggcggatagt ccggtgctgg
    11281 cgaaaccact ggggtagatt actgtcacgt tgcgcgttgc ttattgccgg atgcggcatg
    11341 aacgcgttat cggcctgcgt ttccggcaca ttttgcaggc ctgataagac gcggcaagcg
    11401 tcgcatcagg catgatgcgc caattgcctg ggttttttac tcttgtggcc ataaccacgc
    11461 agcgccgcgt acgccgctgg aatcaccgtg cttcgcctta cgcaccggcg tttcacattc
    11521 gccgccgaag acaaattgtt taatcaactg cggaaccgtt tgatataaac ggtctacatt
    11581 gctcatcccg ccccccagga caatcacatc cggatcgaga atattcacga catgtgccag
    11641 cgattttgcc agccgcagct cgtagcgacg caatgccagt tccgctaccg gatcgctttc
    11701 ttcaaccagg cggataattt cactgccttt cagcgcatgt ccgctcaaac gatgataatc
    11761 cgtcgcgaat cccgtgcccg aaataaaggt ttcaatacaa ccttgtttac cgcaataaca
    11821 agggacttcc tcgcgataac gcagttcgtc ttcgtccatc cacggtagcg gattgtgccc
    11881 ccactcgcct gccgtgccat tgccgccgat atgcgcccgc ccattgaatg ccacgcccgc
    11941 gccgcatccc gtgccgataa tcacggcaaa taccgtctgt gctcccgctg ccgcgccatc
    12001 tactgcttct gaaaccgcca gacagttggc gtcatttgcc agccgcactt cccgctgcaa
    12061 ccgcgcgctt aagtctttat cgaatggctg accgttaagc caggttgaat tggcattctt
    12121 caccacaccg gtgtagggcg aaatcgaacc aggaatgccc atacctaccg ttccgcgctg
    12181 ccctgtcgcc tgctccgcca tatcaaccaa cgtggcgatc gtttcaatag tctgccggta
    12241 atcatcacgc ggcgtgggca gacgatggcg gtacaactgc tcccctgcat cgcccagtgc
    12301 aatcacttca gttttggtgc cgcctaaatc gatacctata cgcacggtac tctccttatt
    12361 tttttcaata tcaatagcgt agagacggac aaccggattg gcaatgcaag gccgccgaca
    12421 attcgttatc atgcccgcta aatttaacga caaggccgtg gaaattatca tgctgtggtt
    12481 caaaaattta atggtttacc gtcttagccg cgagatttcg ttgcgtgcag aagagatgga
    12541 aaaacagcta gcctcaatgg catttacccc atgcggcagc caggacatgg cgaagatggg
    12601 ctgggttcct ccgatgggat cgcacagcga tgcgttaacg cacgttgcca atggtcaaat
    12661 tgttatctgc gcgcgcaaag aagaaaaaat cctcccgtct ccggtgatta aacaggcgct
    12721 ggaagcgaaa atcgccaaac tggaagcgga acaggcgcgt aagctgaaga aaaccgaaaa
    12781 agattcgctg aaagacgaag tgctgcactc tctgctgccg cgtgctttca gccgttttag
    12841 ccagacaatg atgtggatcg acacggttaa cggtttgatt atggtggact gcgccagcgc
    12901 caaaaaagcg gaagatacgc tggcactgtt gcgtaaaagc ctggggtcgt taccggttgt
    12961 accattgagc atggaaaacc cgattgaact gacgctgacc gaatgggtac gttccggcag
    13021 cacggcacag ggtttccagc tgcttgatga agccgagctg aaatcgttgc tggaagatgg
    13081 cggcgtgatc cgcgcgaaga aacaagatct gaccagcgaa gagatcacca accacattga
    13141 agccggaaaa gtggtgacta aactggcgct tgactggcag cagcgcattc agtttgtgat
    13201 gtgcgacgat ggttcgctca agcgtctgaa gttttgcgac gagctgcgcg atcaaaacga
    13261 agatatcgac cgtgaagatt tcgcccagcg ctttgatgcc gatttcatcc tgatgactgg
    13321 tgaactggca gcgttaattc aaaacctgat tgaaggatta ggtggcgaag cacaacgtta
    13381 attgctgatt tgcctttaat gccggatgcg acgcctgcgg cgtcttatcc ggcgtacgaa
    13441 tccacaccag gcatataatc attcgctacg gcgggcaata atttttagcg cagcaatatt
    13501 atgcgtttta cgctgtaact tgctccaggg gcgttgtgtc attgtttttc ctcaagccgg
    13561 tgacgcgctt cactgcgaat tttccgcgtt gccccgattt tcactaaatc ccgtaactgc
    13621 gaaagagcgg gttgatcgac aaataaaagt aatgatggct cctcttttag ccacgccgtt
    13681 ttcacatcgg cagccagctg tggattatta atagccagcg accgatcctg agactcagtc
    13741 aatgtcgtta acaacaccgg ggtatgtgcg ttgcgcaatg ccgtttggcg caaatgaaca
    13801 ttgcgacttt gcagcaatgc acgccaccat tcatcatctg atttatcttt tatcgtcgcc
    13861 agttccgcaa caatatctgg agatcttcgc tgatatttat atcaaccttg atgaattatg
    13921 aatatatcgg aaatcgaaaa aatcccagca aaggaaatat tattatgggt taaagagagg
    13981 acatttcaga aaaatgccct cccgttcaag gggaaggcga ggaattacag atagcggcac
    14041 agataagagg tgggttcggc aacttgcaga tgaaactcgc tgtgaccggg aacattaaac
    14101 accgaaccgg cttcatacac ttgccagtcg gtcgcgtcag gcagtaacac attcagcgcg
    14161 ccactgatta ccgtcatctc ttcaggctca gcggtgctga aggtgtattc accttcaacc
    14221 ataacaccca cgctggcgcg accagtgctg ctgctggaaa agccgatcga tttcactttg
    14281 ccggaaaagt actcattact ttgaagcata aactggccct gtctggtagt tgaacatcta
    14341 cttcactata ggggccagag gcgctggctg tcacgcaaaa ttacaccagt aattccgcag
    14401 ccagccgtgc aatcaatacg ttagagagca agacaggaac atcgagctgt ttttgcagta
    14461 aatcgcgatg acgctggtta aatcccaaac aatccagcat gatgacatct gcaccttttg
    14521 ccagtaattc tttcccggca tcaatgattt tttgttccga atcatgaatg gggttaccca
    14581 atgaaaatac tggcggtttc tgcaaaattt gccatttttg cgcctgaacg gtcagcaact
    14641 cctcaaccgg tacgataacc cccacctgat gatcttcaac aatagaggaa actagcggag
    14701 gcaatattcg cgacggctca agaaagatcg tattacgcgc agtcatacta ctaatgtttg
    14761 ctgtactcat taataaaatg acgtcataac cctgattatc gagcacttca accacacctt
    14821 gcaggtctcg ttccactttg cgacgcgaaa catgggccag ctggttgtca tttaataatg
    14881 tgagaattgt gtcttcacct gcttctggcg cgtactccgc catcacttct tcgcggctta
    14941 acttccccag caggctatga tgggaaatat tatcttcgtc aatgtattct gtcaggagcg
    15001 gcaaaacttc ctgcataggt acaatgccga tggtcaggat cgccaacgac gcactcattg
    15061 ttcttccacc ttacttttat ttcgtcgtta attaccgggc aagcgctaag caccactgct
    15121 gacacttacc tgtgctacaa atgacaaaaa gcgtagcagc agacctgcac ggcataatgt
    15181 aaagattcca aatgattcca gtaatggatt gttattgttt aatattctaa ttatttgaaa
    15241 aacatgaatt gtgaaaaatg tgacgcagat cccttgtggt ggcactcaga atcccttccg
    15301 agcagtctga tgcggtcact ctttgtcagg gtcctcatag cgttttttcg catccatcgc
    15361 ttcctgagcg gtcggatgtt cactgatcaa cgagtctggt ttaggatgat cggctctgag
    15421 ttggtaccag gttaccgtct gtcctggctt tcctttttca atcgtcacta tatatgcttc
    15481 acgaggataa ggcggtttcg ttggcatcgt tcttccttat ttcacgggat gaacgttaag
    15541 tataggcgct cgaaaatcaa caattgatcg tctgtgccag ggcgctgcga atttcagaaa
    15601 tcacctggct gggttcgttt gttgcgtcga tgataatatg cgcaacttcg cgatatagcg
    15661 catcgcgttc ttccagcact tcctgaactt cttcgctcag cggtttcccc gttaaggttg
    15721 gccgtaaatc ttcttccggt gcagcttgca gtcggttaac caggactgat actggcgcac
    15781 acaaataaac cacgatcccg ttattttgca taaagtgacg attaaattcc gtcaggataa
    15841 tgccgccgcc cgtggcgata acggtggatg gcgcagttac cgcttccaac gccgccgttt
    15901 ctctggcgcg gaatcccgcc cactcttccc tttcgacgat ctccgcgacc gtcatattga
    15961 gctgtgattg caaccactgg tcggtatcga caaatcgacg gttaagcgaa tcggcaaggg
    16021 ccattccgac cgttgtttta ccacagcccc gaggcccgat cagaaaaaga ggttgtgtca
    16081 tcgtgggttt tccccaatag gtcgcaatgc ggcgaaagcc ggtgtcatga gaatagcgat
    16141 cataccatca aactagtaca atttcgattg taaagaaaaa attccactta aagtgaaaat
    16201 tttaatacat cccttactat accaataaat tttcaagaat gaagtgtaaa taataaatta
    16261 catttagcca cgactacgtt gcacttccag ccaccacttc tccagctccg cggcaaaggc
    16321 ctggcggtca cgttgtgaaa ggctatctgg tcccccggtc tggatcccac tggcgcgtaa
    16381 ggtatccata aaatcgcgca tcgtcaggcg ctcacgaatg gtcgctggtg tgtaacgttc
    16441 gccgcgagga ttaagcgccg ccgcgccttt ctcgatggct tcagcagcca aaggtatatc
    16501 tgcggtgatc accaaatcgc ccgcttcaca ctgccggaca atttcgttat cggcaacgtc
    16561 gaaacctgcc gcgacgcgca gcgtacgaat aaatcgcgat ggcggcacgc gtaaactctg
    16621 gtttgctacc agtaccagcg gcatctgcat acgttccgcc gcgcgataca aaatctcttt
    16681 aattacattg ggacacgcgt cggcatccac ccaaattgtc ataaagtcat cctttgttgg
    16741 gtaatcctct attgtgtcgc gcttttgcct tccggcatag ttctgtttat gcttctgcca
    16801 gcgattatca aaacaatgaa tttcacggca ggagtgaggc aatggaaaag aaaatcggtt
    16861 ttattggctg cggcaatatg ggaaaagcca ttctcggcgg tctgattgcc agcggtcagg
    16921 tgcttccagg gcaaatctgg gtatataccc cctccccgga taaagtcgcc gccctgcatg
    16981 accagttcgg catcaacgcc gcagaatcgg cgcaagaagt agcgcaaatc gccgacatca
    17041 tttttgccgc cgttaaacct ggcatcatga ttaaagtgct tagcgaaatc acctccagcc
    17101 tgaataaaga ctctctggtc gtttctattg ctgcaggtat cacactcgac cagcttgccc
    17161 gcgcgctggg ccatgaccgg aaaattatcc gcgccatgcc gaacactccc gcgctggtta
    17221 atgccgggat gacctccgta acgccaaacg cgctggtaac cccagaagat accgctgatg
    17281 tgctgaatat tttccgctgc tttggcgaag cggaagtaat tgccgagccg atgatccacc
    17341 cggtggtcgg tgtgagcggt tcttcgccag cctacgtctt tatgtttatc gaagcgatgg
    17401 ccgacgccgc cgtgctgggc gggatgccac gcgcccaggc gtataaattt gccgctcagg
    17461 cggtaatggg ttccgcaaaa atggtgctgg aaacgggaga acatccaggg gcactgaaag
    17521 atatggtctg ctcaccggga ggcaccacca ttgaggcggt acgcgtactg gaagagaaag
    17581 gcttccgtgc cgcagtgatc gaagcgatga cgaagtgtat ggaaaaatca gaaaaactca
    17641 gtaaatcctg atgacttttg ccggacgtca ggccgccact tcggtgcggt tacgtccggc
    17701 tttctttgct ttgtaaagcg ccaaatctgc cgatttcaac cactcacgat agtgactcat
    17761 ttgtgggttc agcggcgcaa cccccacact aatccgtaaa gttacctgtg gcgtattcgg
    17821 cagacgtaat gtatttagcc cttcatgcac tcgtaacatg gcggtaatgg cgctctcagc
    17881 tggcgtaccg gacatgatta ccgcaaactc atcgccgcca aaccgaccaa tcacatcgct
    17941 accgcgcagg gttatttgta actgtcgggt aagcgccaca atcgcttcat cgcccacatc
    18001 atggccccag gtatcgttga tgctcttgaa atggtcgata tcgataatca gtaatgtcgc
    18061 atcgcggtta tgccgccgac agttatcaaa ttcattgcgt aacatagttt cccaatgacg
    18121 tcggttatac acgcccgtca taccgtcgcg ggtactcatg acctgcaacc tgcgtttgtg
    18181 ttccgccagt ttagtcgccg tctggtagct gacccagcca aacagcagag gataaatgac
    18241 gataatggga agggagagcc accattccag cggcgcacta ttgaacgaca cggtgatgcc
    18301 cgtcagttcg agggtgacaa ggcaggaaac caccatcaac accagacccg cgacaaacag
    18361 acgggggccg cctgccccca tcaaattcag acacataatc atcaacatcg cggtggaagg
    18421 cagcacgttt acgcccatta cgcctaccca cattcccgct aatactgcat cggtttttaa
    18481 gttgtaaatt tcccggctaa gcggatcgac ggccctgctc gctatctgcc aggctaaatg
    18541 cggccagacg aacgcccagc cgaccaacac cagccaccac cagcccggcg gcgggtgcga
    18601 aaccagcgtt gaagcaatcg gtaagaacat gccagccagg ccaaccgcgc ggggtagtct
    18661 gacgcgacga gcgaagcgca gcccggatcg ctgatgttcg ttttgaggag ttaaaggagg
    18721 ttcctcccca tgcgccgccg cttttttgaa aaagttttca tcattcatta tttttgggaa
    18781 cattcagaaa caattttccc aaattataga gacggggcta aaggcagaag tatgaaattt
    18841 cgggtgagcc attttcgtag ctcacccgtt agcactatca ggtatcaggc ggctttcttg
    18901 aggcaggcgc tcataaactt attacgatca tcacctttca gagattgttg cgttgcttga
    18961 ttattacatt cgcgcatctt ttgctgctgt ggcgtcaaac ttttttcacc aggcgcagac
    19021 ttgctgttct tcaggcaatc actcatgtag gtcttacgag catccccttt caacgcctgc
    19081 gccgtcgcct gctgattaca ggaggtcata cgctgttgtt gcggggttaa ggttctctcg
    19141 gcagcgccga cggtggttaa aaaaaccagt ccgaaaagca aggtaaccag taatgttatt
    19201 ttcatagcac catccctctt catgttttaa ccatgagcgt ttgcgcccgt gatctgccat
    19261 taagtctggt tgctaacagc aaaaaaacca cccgacagcg aaaattcacc gccgggcgcg
    19321 gttttatttc agccccaggg cggctttcat ggtgtagaag agatcggtct ggtcggtcag
    19381 tccaacgaca ttggcggcat gtgggccata cgctgcaata cgcaactgac tgccggtatg
    19441 ttcttgtgaa tcctcttcgg agttcccgta actcatcacc atcactgcgc catctttggt
    19501 atttagcgcc tgggtgaggc ccggcgcttt ggtgtccggc gcgacaatct ggctggcgtg
    19561 ggcgtgatca gcggtgacta tgaccagcgt gttgccatcc tttttagcga attccagcgc
    19621 acgttgtacg gcttcatcga gatcgaccgt ctcgccaatt tgcccacaag gattcgcagc
    19681 gtgatcctgt ttatcgattg atgcaccttc aacttgcagg aaaaagcctt tctcattttt
    19741 actcaacaat tcaatggctt tgtcggtcat ctgcgccagg gtcggtacgc tgtcattacg
    19801 ttgcggatta ggcgtacagg taactgcggg cttgtcgata ttgccgtggt acgttgcttt
    19861 cggtcctagc cagcgcactg gcatattgcc gtcagcaaac agtcctaata ggggtttttg
    19921 ctgattcgct tccgtcaccg aattcagtga ggcagcatca ctcaccaact gataaccacg
    19981 cgcctgtgcc tgttcacgca gcgtttttcc ctgccattca ccggcggttg ccgtttcagc
    20041 aaaggttttt gcgccgccgc caagcgtaac atcggcacgg gcgttaagca gctgttcggt
    20101 aatcgatcct tttccgcctt tttccagagc gttacccgga catttttcac tggtcgcgct
    20161 cggaccgtag catttgcgcg aggtcacatg cgccaccagc gcagcgggcg tggcatcctg
    20221 caactctgcg gtagaaacgt taccggtcgc cagacctgcg gcttttgcca tttccagaat
    20281 cgttgggtga tctttttcgt gaatatcgat gcccagcgcg ccgttatagg ttttgacacc
    20341 agttgaccag gcggttgctg atgcagccga gtcggtgacg tagtccggtt tgccggtttt
    20401 tttattcagc gcatagtgag tgtattgccc ggtaagcggt aaggcatcga tacctttaaa
    20461 aaagccgccc gcaccttcgg cataattgcg tgcggcagta atttccgagt cccccatccc
    20521 atcgccaatc agcaaaataa tattttttgc aggtttatcg ctaagagaat cacgcagagc
    20581 ggcggtctga tcacccgtta agcggcgagc accgccgggc gcagtaatat cgccctgagc
    20641 agcccggttt tccagaacag gcatttctgg tgtccgggct tttgtcacag gggaaaacag
    20701 taacggtaag agtgccagtg caatagtgct ttgtttcact ttattttctc catgtacaaa
    20761 tacattaaaa ataaaaacaa agcgactata agtctcggcc gtgacagctt tatgacagct
    20821 gttgaaaaga ttaacttttt actgacgagg atgcttcaat aacttcttta cataattgcg
    20881 cagcagctcc gtatcgtcgt caggaatgct ggcatcgggc tttacctcgt acagcgcccc
    20941 ctctacctga tcaatcaacc tctgttggtc attttgcgcc atattgcgaa gcattgcagt
    21001 gacgataatc tccaaagctt ctacctgcgc acacagttct ttcgactctt cttctttttg
    21061 ggcaagctta aataacaact cagcaatgag atttttcatg tctgtatttc cttatccaaa
    21121 gtatggagaa gttaacatta tgtttcacaa cttcatagtg tctggaagta ttatttttat
    21181 gacaaaggaa atactttgcg caaatatttt ttgtttgatt accagcgaaa cgtttctctt
    21241 attttaataa ttacccacac aaaatatagg cttttaatta tcaaccttta ttataaataa
    21301 aagttatgaa atatattctc ttcgttaact tataattaag agaaaaaaac tcactgcatg
    21361 gccatacaag ccaggaataa attagcttcc cgttcatcat ttttgtgatc ttgcgcacat
    21421 cattgagata acgcactttc ttcaccagta cgttgcacgc tttgtcagca gaagattgca
    21481 acattctgtc agaaggccgc gaatcccccg ttttgtcgtg gtaatgtatg cactttgtgt
    21541 tagacgggat aggtttttaa gatggaaaaa ctgcgggtag gaatcgtttt tggtggtaaa
    21601 tcagcggaac atgaagtgtc tctgcaatcg gcaaaaaaca ttgtcgatgc cattgataaa
    21661 agtcgcttcg acgttgtgct gctgggcatt gataaacaag ggcaatggca cgtcagcgat
    21721 gccagcaatt atctgctaaa tgcagacgat cctgcccata ttgcgttgcg cccttcggcg
    21781 accagccttg cgcaggtgcc tggtaaacat gaacatcagc ttatcgacgc gcaaaacggt
    21841 cagccgttgc cgacggtgga tgtcattttc ccgattgtcc acggtacgct gggcgaagat
    21901 ggttccttgc agggaatgct gcgggtcgcc aatttaccgt ttgtaggttc tgatgttctg
    21961 gcttcagcgg cctgtatgga taaagatgtc accaaacgtc tgctgcgcga tgccgggctg
    22021 aacattgcgc catttattac cctgacgcgc gctaatcgtc acaacatcag ttttgccgaa
    22081 gtggagtcta aactggggtt accgctgttt gtaaaaccgg ctaatcaggg ctcttctgtt
    22141 ggtgtcagca aagtaaccag tgaagaacag tacgcaattg ccgtcgatct ggcgttcgag
    22201 ttcgaccata aagtgatcgt tgagcaaggg atcaaaggtc gtgagatcga atgcgcagtt
    22261 ctgggcaacg acaatccgca agccagcacc tgtggcgaga tcgtactcac cagcgatttc
    22321 tatgcctacg acaccaagta cattgacgaa gatggcgcga aagtggttgt tccggcagcc
    22381 attgcgccag aaatcaacga taagatccgg gcgattgccg ttcaggccta tcaaacgttg
    22441 ggatgcgcag gcatggcgcg tgtagacgtg tttttaaccc cagagaacga agtggtgatc
    22501 aacgagatca acaccctgcc tggcttcacc aacatcagta tgtatccgaa gttgtggcaa
    22561 gccagcggtc tgggttacac cgacctgatc acgcgtttga ttgagctggc gctggagcgt
    22621 cacgctgcag ataacgcact gaaaaccaca atgtaatatt caaacgcccg gtaatattct
    22681 tattgggcgt tttcagtttc ttcatcgcga cgacgaataa tcagccccgc cagccagaaa
    22741 ctaatcaccc acgtcaccag accgacggca taggtctgcc aaccttttgc ttcaaatccc
    22801 aataaaccca ccacgccatt aagaatgaat ataaggccaa tggcgaacgc atagtagtgc
    22861 cagtcacggc ggatttttac aggcaagttc atggcagctc cgttaacgaa aaacaacatc
    22921 cttgcatatt ctgcaacatc cgtctgtggc gtagacaaca tttccgtaat tgttaataaa
    22981 tttaacttaa ggttgtcatt ttgtgactca cgacgaagat tcacgcttcg ggaatattcc
    23041 caacatcaat ttaccatgat tctgatattg acaatcttaa tgacctgtca gacttgccat
    23101 aagccgctgg tatcacacca gaagagaagc agatgacttc cggaggtttt tatggctgat
    23161 ttcaccctgt caaaatcgct gtttagcgga aaatatcgca atgcctcttc aacgcctggc
    23221 aatattgcct atgcgttgtt tgtgctgttt tgcttttggg ctggggcgca attgctgaac
    23281 ctgctagtgc atgcgcccgg cgtctatgag cgtttaatgc aggtccagga aacaggtcgc
    23341 ccacgggtgg aaattggttt aggtgtcggc accattttcg ggctgatccc gtttttagta
    23401 ggctgcctca tttttgcagt agtggcgtta tggctgcact ggcgacatcg ccgccagtaa
    23461 gatttacttg ccagatagga cctaaaacgc tttatctggc atgacattcc tgtttgatca
    23521 ctgtttcata caacgcgccc gccgttcgtc tacgcgcttc gcaaaccagg ccgtagtcag
    23581 gttgcgggtg attttcgggc tttccagttg aatgccaggc aacatttcgc ggggtagtga
    23641 tttacccgtt ttcgcttcgg caagttgata aactttcttg tacagcgccg tctcctcaaa
    23701 agagaagctg tcccctttct ctaactggcg gcgaatttca ctgtcgctca tccccagttt
    23761 tcccgccagt ttgcgcgttg ccagttcggt tttcccgggc tctttactgt cgtaacgaat
    23821 taaatcaccg tccagtgcca gcttcacgcc gctgacctta ctgaccgcgt tctgaaacgc
    23881 ggcattacgg ctggcgtacc agccagcgtt aaaatcagca aaacggtata tcggcgcgct
    23941 atagctggcg ggataattca gtaaatggta agtaccaaac cacaacccgc cgcgacgact
    24001 gaagacttcc tgacggactg taccgtccat tttccacgga taccctttgg tatgctgttc
    24061 ggcaaaggca atgctgacct gcatcggccc accggtgcgc accggattaa gcgaaccaaa
    24121 caacgtctgc cccattggca ccatgctgat cagatcgtca aaaatcgcgc tcaattgctt
    24181 ttcagtacgc accgaatcta accgttcgct atagcttttg ccgtttggcg atttgatttt
    24241 tagcgcggta tgcaccagaa aagcaggaat gtgcatccgt tcagcacgac ggtcaatctc
    24301 ttgccaggcg atcttgctta agcccggaac ggccggatct gcctgataat ttgactcctg
    24361 ctgcgccacc gccagcaccg agcagacgtt ttccagcgtc ggtgccagcc cctgactttc
    24421 aaacgtggtg gcgagatctt tcgcccaggc atcgcggtct ttcacgctcg caggcatctt
    24481 ctgccgcacg acgctcgcca catctatcgc tttttcgccc tttttcaacg gttgcggagc
    24541 ctgactacta caccccgcta aaaccagaac cgccaacaat gacagcgaag atagatgatt
    24601 gacgcgcgac attacttctc ctttttagct caaggtatgg gttacttcct gaattttgtc
    24661 accatccagc tcatgttcaa agctgcgcag acgtttgtag atagacatca actcaaccag
    24721 tgtggtccat gagttaatca ggtactggaa agctccgcga acctgaccaa aaacgttggt
    24781 aatctgcgtc atcaggccga gcgtaatcgt accggcaaca attgacggaa acagcaagaa
    24841 caaaccgaaa acgttatcga cctgcaaata gagaatgcgg gcgatgttga aatacatata
    24901 gtgaaaatag aggcggaaat agtttttccg tacggcgcta aacagctcgc gtaccgtagg
    24961 cggcgtcgcg cgcgtggcat cgtcttcacc ataaaccagc tctttacggt aggcagcctc
    25021 tacacgctgg tttttaaact ccagccccgg cagtttgatc cctaccactg ccagtaatcc
    25081 ggtccccatc agcgaccaga cgatagcggc aatcaccaga ccatacggaa tgtgcccgac
    25141 aatcggcagc tccggcacat gcgcggagag cgttaccagc accggcagga aggcgatcaa
    25201 cgtcatgata gcgttgataa agctgacgcc catattctcc agcgttgaag caaaacgcat
    25261 ggtgtcttcc tgcacacgct gtgcggcaac ttcgatatga cgcagttgtt gccagttcgc
    25321 catgtaatat tcgttcatcg ccgtacgcca gcggaacacg tagtgactga caaagaagtt
    25381 gttcagcaca ctgatcacca ccgcgatcag cgcaatcccc agaaagacgc ccacttcgcg
    25441 gtaaaattgt tcgatggtga ctttatgcgg cgaacttagc gcggtttgaa tcagatcata
    25501 gaacggcgca taccaggcgt tgacggcgac cccgacttcc accaaaaacc aggtgacgaa
    25561 gatgatcagt gcagtaccga gtattgacca gtattgccaa cgatgcgggc tgtagataaa
    25621 ccagaacaat gcaaaaagtc ctacgcaaac aatgtagtaa gcgtaaaaaa tcaagaaatc
    25681 caacgaccag aaacgcgcgg cgctaatcgg gatctgcccg gaagctccgg tgatacgcgc
    25741 cacccaatcc cccccaccgg cttgccagaa gataacggca atcaatgccc aaacaaaggc
    25801 cgagagaaaa aacggtcccg gctttgggaa aaaagactta aacatatgca ctcctgctaa
    25861 cttcttatcg ttttaacaac tgctgtgtat taaccgcatg accgctacaa agcgtcagcc
    25921 aaatgaaacc gcccattgtg acccatagca aggcgcttag ttcgccagcc tgagcataag
    25981 aattgtttcg acggatttca caaaccaaag attgtttaca taagcggatt aacacgcgca
    26041 ggatcacaaa tgcaggcatt ggtgagcagg aaaaagatcg tgtttacgcg ccgttacgtt
    26101 tttaacacta ttattgcact aattcgccct ttgcaatcca tcaatgagta gtataaatac
    26161 gctcagttac cttcattcaa tttatggaca ccaccgttga aacgtagtct gcttttttct
    26221 gccgtgctgt gtgcggcgtc attgacctcc gtccatgcgg cgcagccgat cactgaaccg
    26281 gagttcgcct ctgatattgt cgatcgttat gccgatcata ttttttacgg cagcggtgcc
    26341 acggggatgg cgctggttgt tatcgacggt aaccagcgcg tctttcgcag ttatggcgaa
    26401 acgcgacctg gtaataacgt tcgcccgcag ctggattcgg tagtgcgtat tgcttccctc
    26461 accaagctaa tgaccagtga aatgctggtg aaattgctcg accaggggac cgtgaagtta
    26521 aacgatccgc taagtaaata tgctccgcca ggcgcacgcg tgccaaccta caatgggacg
    26581 cccattacgc tggtgaatct ggcaacccat accagcgccc tgccccgtga acagcccggt
    26641 ggcgcggcac atcgtccggt atttgtctgg ccaacgcgcg agcaacgctg gaaatacctt
    26701 tccactgcca aactaaaagc cgcgccgggc agccaggccg cgtactccaa cctcgcattc
    26761 gatctgctgg cagatgcact ggcgaatgcg tccggcaagc cttataccca gttgtttgaa
    26821 gagcagatta cccgtccatt ggggatgaaa gacaccacct acaccccctc gccggatcag
    26881 tgccgccgtt taatggtggc agagcgcggt gccagtccat gcaataacac gctggcggca
    26941 attggcagcg gcggcgtcta ttccacgcct ggcgatatga tgcgctggat gcagcagtat
    27001 ttgtcatctg atttttatca gcgcagtaat caggcagatc gcatgcagac gttgatttat
    27061 cagcgcgcgc agtttacgaa agtgattggc atggatgtcc ccggcaaagc ggatgcgctt
    27121 ggtttaggct gggtatatat ggccccgaaa gagggtcgtc cggggattat tcagaagaca
    27181 ggcggtggtg gcggtttcat tacctatatg gcgatgatcc cgcagaagaa tattggcgca
    27241 tttgtggtgg tcactcgttc tccattgacg cgctttaaaa atatgagcga tggcatcaat
    27301 gacctggtga ccgaactgag cgggaataaa ccgttggtca tccccgcgtc ctgattaata
    27361 ctctgaaggc aaacggttga tagcgaccag tttgccttta ttcatttcga tatagccgcc
    27421 tttacgtaaa gcagcgagaa cttctgcgac gacagaacgc gatatgcgtg tacgttgatg
    27481 gatataattc atcacaccaa tacgtgagcg taattcttca ttccagtcaa tcattgataa
    27541 taacgttgcg cggatttgtt cgtaggaatt atgcccaatg agctgtacgt cacgtaattc
    27601 cagaattcta ttttgccagg ctaaccagta aaaagcgtct cgccagagtt gattttgttc
    27661 aataagggtg atggtttgtt tggctggcag atgatatccc gtacaatttc cttctgatat
    27721 taatttgtat ggtatatcgt ttttcattaa accatcagct agccccataa tataaggagc
    27781 ctgggtaata ccgataagta cgttttcttc tctacgcaga gagataacgc cctctaaaat
    27841 aacgaaagta tcttcgctat tgacatcact tgaaaatata acttgctttt cattattaaa
    27901 ctcgaagcgc gtgccatatc tggacaaaca tttatcgagc ttaccaaatt cctgaagagg
    27961 tttaactaca gataacattt gcgcgtcctt tgcagtaatg cccgtcaaat ccttgacggg
    28021 cattatttag attaaataac ctatttcttc agagtgaaga atattaccag gtatatttaa
    28081 cacccacgtt cgcggaccag tcttgatcta cgtcaccacc accgaggtag ttagcatcgg
    28141 tataggcgct gaagttcttg gtgaagctga actgagtacc cagaccaaca cgtaccgcag
    28201 acccttcagt accgttatcg atggaatcac cgttcacatc gttatggttg ttagagtcgt
    28261 cgtaaacgta agccagtttg aagtacggag tcagagcctg gtcttcgctg taggtgaagg
    28321 tataacctgc atctacaccc agttcataac gcatgctgtc gtaagactga ccgtcaactt
    28381 tcatgttgtt gctcagctgg tagtcatcac cagactggaa cagaccagaa acgctgccgt
    28441 aaggagtcac gtaaccagca tcacccagtt tgaagtcgta accggctttc aaaccgaagc
    28501 cccaagcgtc ggagttggtg ctaccatcaa cataagtacc gttgctcatg gttgcagaca
    28561 ggtcgttgtt gaagtgagag tagctcaagc taccatcaac aaagacgttg ttcgcgaagt
    28621 gagcagaaga gtagatgtag gcagtctggc tgtcttgatc cacctgacca gaacggtcat
    28681 tcatgtcacc tttagcgaag cctgcagccg caccgacgat ccacttagcg ttgttaccgt
    28741 caattttggt atcaacaccg accatgatgc cgttaacatc ctgatcatag ttgatggtgc
    28801 cgttgtcgcc gttgaagtta ccaccgaagt agcttaccca tgcgccgccg ttatcagcca
    28861 ggccatgacg agagttggtc agacgagtac caacggtgtc ttgttccagg ttccagatat
    28921 tggtgttcgc agacgggatg ctcagcgcca tgttagcgta gtcggtcagc tccatctgtt
    28981 gcagaacaac ggtgttacca cgctgttcag cctgataggt gtatgcaccc aggtcagctt
    29041 tgttagcagc agagaaggtc gcgttgctgt tgacgtcgtt tacgtagata atttctttat
    29101 ctttgtaatc agcgattgcg ccagcgccag ttgcgttgtc gatacgaact ttatagttac
    29161 cggttgcagc aacaacgttg tctacagagc tgttatccag ttcgtcgccg ttgttcatgt
    29221 cgccgttacc attgatagtc agatgaccat cagagttcat agcaacaata ccgtagccgt
    29281 agttagactt ggaagtatcc caggtacggt cgttcaccaa gtcagcgttc agaacataat
    29341 cgctgctgtg gatatcgaat acaccggata ctacattacc attattgtca gcaatgttgc
    29401 cgctggtcag ttccagtttg tcagtacgga aagcttcagt atcgaactga tcaacgttca
    29461 catccaatac accaccgtta gttacggtga tggtattggc gtacaggttg taatcagcac
    29521 cccaacctgt agattcggtc aacgcaacag tactgtagga gttaatagtc agatgatcgg
    29581 tatcaacgta accatcttca ccaatgttca gcgaagaacc gttagttaaa tcgatagtat
    29641 ctgaagttaa tttagattcc gaaacattaa cctgagaagc attattaact gtcaaggtat
    29701 caacgacgga acgacctgta gtatcccatt cagaaccgtt gttcaacgct acgttaaata
    29761 caccactctg gaatacttta ttatcgatga tgcgattcca accattagct tcagctgttc
    29821 caccaggggt caaactgtta gttgcaacat catacattgt gtctggagaa atggtttcag
    29881 cattgaatgt agcctgtcca acccacttgc ttccattatc aagagtaata ttcagctcat
    29941 caacgttcag gctatcatca acccaaccag cattggtatc ttttacgccg tcaccgttag
    30001 aatcatgacc acttggatca aaattagcat tccaggtgct ggtaaacgca acatcaccca
    30061 ccagagtaga attgctgaaa tatacattgt tcttcattgc atagtctgaa gtactgtcag
    30121 tgaaagacaa tgcaacgtca cccggaccag cataatcact cggctcacca gagttaccga
    30181 aatgaccata accagaactt tccaacacgg tatttgaacc ggtggttacg gttgagttag
    30241 agacattaat attgttatcc caagtatggg attctgtagt gtaagtattg ttgtaagtac
    30301 taccttgaga taacgcaata cctgcaacac gggagttatt ggagatattg atgttacttt
    30361 ctacattaag attaacagca acgccttcgc taacgtcaaa agtgccgtta gtagatttag
    30421 ttacatcgcc atctttgtag taatcagtaa agtaaagtgc ttcatagtca tcatcaatag
    30481 tggagttagc aatattcaga gtgaatacat caccgtcata ccacgttgta tcaataactg
    30541 tatcaccagc ctgaatgtag ccatagcttt gatcataagc attattatgc cagagatcac
    30601 caccaggcag catagaagtg attgagccgt gaattactga attagcaata tcaagctggt
    30661 tagcattgcc atttgtacca tcagtgtagt cgtggttcag ataatatgta gaaatcgtgc
    30721 cgttaacagt atcaccgtta aaagactgat cgaagtcgcc attggtgttg ttatagtaga
    30781 caaagccact ataattcaca tagttaccgt ctacagtgcc cggattatcc atattggtcc
    30841 agttaagacc agtagccgcg ttatcgacat atgtccaggt gtcataagta gttgcattag
    30901 cctgagaggt gatagccaga gtgcaagcca atgctaattg tgatactaca agtttctttt
    30961 tccaggagtg catttgtcat ccctcctcag ggacgtaata agttgatcca tcaattatca
    31021 atgcatagaa aacatattat gtttcgcgct aacgattatg cggcgttatt gaggaatggt
    31081 tcaattattc tttccccgaa gtccggttac ggacatatat aaagcacgga ggaacacaca
    31141 aagaaaaaat tgataaaagc ttaatgtttc aacaagttaa caggagttcc atttttaaag
    31201 catatgcatt taaaatatca aacacacaaa ccagacaaat aatctatcaa taaccaatga
    31261 actttaaatc attccgtttt tgaaaacgtc accaaatccc caaaaacaat aatctatagc
    31321 tataaataaa aattaaaaat attttcaatg aatataaatt tattgctatt tctattacaa
    31381 agatgtcctg acctctcatt cattctcttc ttgtcaacat cgcgacaact ttcgtaaaac
    31441 atccccaccc tgcttcaggt atactatgcc cctcgattcc acaaacatca ggcagaccat
    31501 gaccgactta atccaacgcc ctcgtcgcct gcgcaaatct cctgcgctgc gcgctatgtt
    31561 tgaagagaca acacttagcc ttaacgacct ggtgttgccg atctttgttg aagaagaaat
    31621 tgacgactac aaagccgttg aagccatgcc aggcgtgatg cgcattccag agaaacatct
    31681 ggcacgcgaa attgaacgta tcgccaacgc aggtattcgt tccgtgatga ctttcggcat
    31741 ctctcaccat accgatgaaa ccggcagcga tgcctggcgg gaagatggtc tggtggcgcg
    31801 tatgtcgcgc atctgcaagc agaccgtgcc agaaatgatc gtcatgtcag acacctgttt
    31861 ctgtgaatac acttctcacg gtcactgcgg tgtgctgtgc gagcatggcg tcgacaacga
    31921 cgcgaccctg gaaaatttag gcaagcaggc cgtggttgca gctgctgcag gtgcagactt
    31981 catcgcccct tccgccgcaa tggatggcca ggtacaagcg attcgccagg cgctggacgc
    32041 tgcgggcttt aaagatacgg cgattatgtc gtattcgacc aagtttgcct cttcctttta
    32101 tggcccgttc cgtgaagctg ccggaagcgc attaaaaggc gaccgcaaaa gctatcagat
    32161 gaacccaatg aaccgtcgtg aggcgattca tgagtcactg ctggatgaag cccagggcgc
    32221 agactgcctg atggttaaac ctgctggagc gtacctcgac atcgtgcgtg agctgcgtga
    32281 acgtactgaa ttgccgattg gcgcgtatca ggtgagcggt gagtatgcga tgattaagtt
    32341 cgccgcgctg gcgggtgcta tagatgaaga gaaagttgtg ctcgaaagct taggttcgat
    32401 taagcgtgcg ggtgcggatc tgattttcag ctactttgcg ctggatttgg ctgagaagaa
    32461 gattctgcgt taatgatcat tatgccgggt tatccaaccc ggcatttccc ccttaccccg
    32521 cccgataaaa cggtttatcc ccaaggatcg tcgcccgatg cataatccgc cgctgtggca
    32581 ggtaatccgc attggcatag tgctgagtca cgcggttatc ccaaatcgcg atatcatttg
    32641 gctgccaacg ccaacgcacc tgaaactccg gtttggtgat atgggcaaac aaaaaactta
    32701 acaaggcttc gctctctttc tcgctcacat caacaattcg cgtagtaaag ccttcattca
    32761 caaacagcgc ctgtttaccg ctcaccggat gcgttcgcac caccggatgt agcaacggcg
    32821 gatttttcgc gaccgcctcg cgccagcgtt gatgctcctc ctcggttttg cggtatttgt
    32881 attccgggaa cgatttacgg aaatcatgct ccgcacgcag cccgctcagc agctggcgga
    32941 agggaacaga gagcgcctca taggccgcaa taccgctggt ccagagcgta tcgccgccgg
    33001 tcgagggtaa ctcttttgcc gccagaatcg cccctgcggg tggcgtttca ataaatgtca
    33061 catcggtatg ccagttgtcg ttatctggcg gattatcgtt atgggtatcc aggacgatga
    33121 tctcgtcaac cccttcggca tgagggtaaa cagggtgaat atgcagttcg ccaaaacgct
    33181 gggccagcgc gcgttgctgc tgcggcgtaa tagcttgatc gcgcagaaac accacctgat
    33241 ggcgtagcac cgcatggtaa agctgttcaa actgattatc gcttaacggg cgcgtcaggt
    33301 cggcacccga aatttgtgcg ccgatatacg gccccagcgg ggtaatgctc agacgttcac
    33361 tcattgtact tctccatgcc agggcgtcag acggcgctgt aacgcgcgca aacccagttc
    33421 taaaagaaag gcgataatcg caatcaccgc gatccccgcc agcaccacgt cagtcgcgag
    33481 aaattcaccc gctgactgaa ccataaatcc taaaccgcgc gtcgcggcaa tcagctccgc
    33541 cgctaccagc gtagaccagc ccacacccag accaatgcgt aatccggtga ggatttccgg
    33601 cagcgcgccg ggcaaaatga caaaccacag cacctgcgca cggctggcac ccagcgactg
    33661 ggcggcacga atgcgaacct gctgcgcgct tttcaccccc gccagcgccg acatcgctac
    33721 cggggcaaaa atcgctaaat agatcagtaa gatcttcgaa gtttcaccaa taccaaacca
    33781 gatcaccatc agcggcaaat aagccagcgg cggcaccgga cgataaagct cgattatcgg
    33841 atccagaatg ccgcgcaccg tcgggcttag tcccatggcg atcccgaccg gaatgccgat
    33901 caacaccgcc gcgagtagcg ccagcaaaat gcgcgtcagg ctggctgcca gatgctgcca
    33961 cagcgtggcg tccataaatc cctgcggtcc ggcaatggtg agcagttttg ccagtacctg
    34021 ttgcggtggc ggcaaaaaca gcgggctaat cagttgcagc gccgcgactg cccaccatac
    34081 agtgagcaaa accgctaacg taccaatgct taaggtcacc tgacgcgaga gcggccagcg
    34141 ccatttcagc cgatgcgaat gcagtttttc attaatgagc acactcatga gaatgcctcc
    34201 cgttgctcaa atacgcggct taaaacatat tcgcgcatgg cgatgaattg tggatcggac
    34261 ttgatgctgc ggctcggttc cccggcaaca aagcggcgag caaagttgag cggcagccgc
    34321 tccagcacgc ggccagggcc ggatgaaagc agaaccagtt cagtcgccat aaacaccgct
    34381 tcttctatat cgtgggtaat caacagcacc tgcttgcccg tctcctgcca gagtttcagc
    34441 agcagggttt gcatctgatc gcgggtgaag gcgtccagcg cgccaaacgg ttcgtcgagt
    34501 aataacagct ggggattcgc cgccagcgcg cgggtaatcc ccacccgctg acgttgaccg
    34561 ccggaaagct gccagatgta gcgtttttcc gccccttcca ggccgacttt tttcagcatc
    34621 tggtgcgcga tttccagtcg ctgcattttc tctacgcctg ccagttgcag gccgaatgcc
    34681 acgttgtcct gtacattgcg ccacggtagt agcccttcat tctgaaaaac tacgccacgc
    34741 tctgctcccg gtccctcaat acgcttaccc gccagttgaa tgctgccatg ctgataaggc
    34801 acaaaaccgg caatcagatt cagcaaggtg gttttgccgc agccagacgg tcccagaacc
    34861 accagaagct cgccgctttc cagcgtcagg ttgatatctt ccagtgccgg tttgccgcca
    34921 taatcggcgt aaagatgaga gatttgcagc atctgctgcc tccttttatt gcacgaagcg
    34981 cgaggtcacg tactggctgt aatcattcgc cacggcaggg actttaccct gatctttcaa
    35041 aaactgcgcg gtatcgatga tcgctttatt caccggtccg gtcagttctg ccgtttgttg
    35101 ctgcggcgtc agataggtat tccccttcac cagccccgga acgtcacctt caggcacgcc
    35161 gcttaaccgc gccagtttgc tgatgttttc cggctgtttc agccacgcgt ctgggttagc
    35221 aatgtacggt tgctgagcat cgatggcgct tttagcgaac gctttcacga cctcaggatg
    35281 tttctcggca aaatctttgc gcactaccca gacatccagc gtcggcgcac cccactgccc
    35341 gacctgttca gaatcggtca gcaccttgcc gtctttttcc agggcgttaa ccgccggtgc
    35401 ccagacataa gcaccatcaa tgtctccccg ctgccatgca gcgataatcg cgggcggctg
    35461 caggttcaca atctccactt gcccgggttt aataccccag tgtttcagcg ccgccagcag
    35521 gctgtagtgg gtggtggaga taaacggtac ggcgatgcgc ttgccaatca gatcttccgg
    35581 tttgctgata gttttcttta ccaccagcgc ttcggagtta cccagttttg acgccagcaa
    35641 gaagacttca atcggcacct gttggctggt tgcaaccgct aacgggctgg aaccgaggtt
    35701 gccgatttgc acgtcgcctg aagccagcgc ccgcacgatg ctggctccgc tgtcaaactt
    35761 acgccagtcc acggttgctc cgctttcttt agcaaaggtg ttgtcggcct gagccacttt
    35821 cgccggttcg gcggaggttt gatacgccac ggtgacgttc accgcctgcg cctgaaaagc
    35881 gatgaatgcc agtgcggcaa gaagtgtgtt acgcgatgaa attgccatga ttgtctgctc
    35941 ccctgtcttg ttatgggagc agtattcagg aataaaaaca ttcattaaaa gaattagtcg
    36001 ttatcgcaca gatgatttta ttcttagcaa aaaaacggca aaggaaagat taaatatgca
    36061 attactccgt tctaacaatt gggaaaatta ctacacttca ttttagtgat gcataaacaa
    36121 ccacattacg agatttatta cttcattatt tctatcaata tcaatacata taataaaact
    36181 attatattcg cattgtatat atcactgtat cattctcaat cactcgcata ttacattcac
    36241 ataatgtata taaacaatac ttgttcacag aacatatttt ttaaatgcaa ttaattgaca
    36301 aactaaatca catctaaata caatgtataa aacaaaataa ctatacatac caaacataca
    36361 aacacttaac atatgaattg cataaaaaca ttacccagtc gaacctccgg gtctgaaagc
    36421 ccttgctttt cagttattag caacatttaa catttataaa ttaacatctg gctgcgccag
    36481 agattacgtc tctgagtgca tcgttgtgct tgtagtataa taaataaaat ggagtgtcat
    36541 taagtggata aggttttaga ttcagccctc ctttcttcgg caaataaaag aaagggtatc
    36601 cttgctattg gcgcacatcc tgacgatata gaattaggct gtggcgcatc gcttgctcgt
    36661 cttgcgcaaa aaggaattta tatcgcagcc gtggtgatga ctaccggcaa ctctggcaca
    36721 gatggaataa tcgatcgcca tgaagaatcg cgcaacgccc taaagatatt agggtgccac
    36781 caaactattc atcttaattt tgctgacacc cgcgctcatt tacagctcaa tgatatgatt
    36841 tccgccctgg aagacatcat taaaaatcaa attccttctg atgttgaaat catgcgggta
    36901 tataccatgc atgatgctga ccgccatcag gatcatcttg cagtctatca agcctcaatg
    36961 gttgcctgcc gaactattcc acaaattctc ggctacgaaa ccccaagtac ctggctttca
    37021 tttatgcctc aggtttttga atccgttaaa gaagaatatt tcacagtcaa acttgctgca
    37081 ttaaaaaaac ataaaagcca ggaacgacgc gattatatgc gtcatgatcg cctgcgtgca
    37141 gttgcacaat ttcgcgggca acaggtcaat agcgatctgg gtgaaggctt tgtaattcat
    37201 aaaatgattc tttgagcaaa ttgcatgaaa acctggatat ttatctgtat ggccgtagca
    37261 atattgctat ggttcctgag tacgttaagg cgtaagccca gccaaaaaaa aggctgtatt
    37321 gacgccatta tacctgctta taacgaaggc ccgtgtctgg cgcagtcact ggataatctg
    37381 ctgcgtaacc cttatttttg ccgggtaatt tgcgttaacg acggctccac ggacaatacc
    37441 gaagcggtca tggcggaagt caaacgcaaa tggggcgacc gctttattgc cgtcacgcaa
    37501 aaaaataccg gtaaaggtgg tgcgctgatg aatggcctca attacgccac ctgcgaccag
    37561 gtttttttaa gtgatgccga cacctatgtt ccgcccgatc aagacggaat gggctatatg
    37621 ctggcagaaa tagagcgcgg tgctgatgcc gtaggcggca ttccctctac tgcgttgaaa
    37681 ggcgcgggtc tgttaccgca catccgcgcg accgtaaagt tgccgatgat tgttatgaag
    37741 cgcacgctac agcagctcct gggcggcgca ccgtttatta tcagcggtgc ctgcgggatg
    37801 ttccgtactg atgtattgcg taagttcggt ttctctgatc gtactaaagt cgaagacctt
    37861 gatctcacct ggacattggt ggcaaacggc tatcgtattc ggcaggcgaa tcgctgcatc
    37921 gtatacccac aggaatgcaa cagcccgcgt gaggaatggc gtcgctggcg gcgttggatt
    37981 gtgggctacg cggtctgtat gcgcctgcat aaaagacttt tatttagccg cttcggtatc
    38041 ttcagtatat ttcctatgct gttggttgtg ctttatggcg ttgggattta tctcactacc
    38101 tggtttaatg aattcatcac caccgggccg catagtgtgg tgttggcaat gtttccgctt
    38161 atctgggtcg gcgtagtttg tgttattggt gcttttagcg cctggtttca tcgttgctgg
    38221 ttgttggtgc ctttagcgcc gctttccgtt gtgtatgtat tattagctta tgccatctgg
    38281 attatttatg gacttattgc cttttttact ggacgcgaac ctcagcgcga caaacccacc
    38341 cgctattccg cactggtgga agcgtcaacc gcttattccc aaccttctgt cacaggaact
    38401 gaaaaacttt ctgaagctta atgttaaaga gaaaaatatt cagattgcag accaggtaat
    38461 tattgatgaa actgcaggtg aagttgttat cggcgcgaat acccgtattt gtcacggtgc
    38521 cgttattcag ggtccggtag tgattggcgc aaactgcctg ataggtaatt atgcgtttat
    38581 tcgtcctggc acaataatca gcaatggcgt aaaaattggt ttcgccaccg aaattaaaaa
    38641 tgcggttatt gaagcggaag caacgattgg tccgcaatgt tttattgccg actcggtagt
    38701 cgcaaaccag gcatatttgg gcgcacaagt acgtaccagt aatcatcgtc tggatgaaca
    38761 gcccgtgtct gttcgaactc cagagggaat tatcgctacc ggatgcgata aattaggttg
    38821 ttatatcgga cagcgttcac gccttggcgt acaagttatt attttgcctg ggcgaattat
    38881 ttccccgaac acgcaacttg gcccgcgcgt gattgtagaa cgtaatttac ctaccggaac
    38941 ttattcactc cgacaagaac ttatccgtac aggagattaa aatgataaaa cggacgttat
    39001 tagcggcggc catttttagc acattgcccg cttatgccgg gttaacttcc attaccgcgg
    39061 gctacgattt taccgattat tctggcgatc atggcaatcg taatttagcg tatgctgaac
    39121 tggtggcgaa agttgaaaac gcaacgctgc tttttaatct ttcccagggg cgtcgtgatt
    39181 atgaaactga acatttcaat gccactcgcg gtcagggtgc ggtctggtat aaatggaata
    39241 actggctgac aacccgaacg ggtattgcct ttgcggataa tacgccggtc tttgcccgcc
    39301 aggattttcg tcaggatatt aacctggcac tgttgccaaa aacgcttttc acgaccggtt
    39361 atcgctacac taaatattac gatgatgtcg aagtcgatgc ctggcaaggc ggcgtatccc
    39421 tctatactgg cccggtgatc tccagctacc gctataccca ttatgactcc agcgatgcag
    39481 gtggtagtta cagcaatatg atttccgtgc gtctgaatga cccgcgcggc actggttata
    39541 cgcaactatg gctaagccgc ggaacaggcg cttacaccta tgactggacg ccagaaacac
    39601 gctacggcag catgaagagc gtcagtctgc aacgtattca accgctgact gagcaactta
    39661 atctcgggct gacggcaggt aaagtgtggt acgacacccc aaccgatgat tataacggtc
    39721 tgcaacttgc agcccatctg acctggaaat tctgattgct tctgccgccc gctatccggg
    39781 gcggccttcc ctgtcgatta ggcccctctt tcctttttgt tatccgacta cattcaccgg
    39841 ataaatttta ttctccagtg ttatatacta taggggggta tgcattgaca tatagcatac
    39901 ccccctatag tatattgcgt gcagatgatg aggtgcgaaa tgcccagtac tccggaagag
    39961 aagaaaaagg tccttactcg agttcgtcgt attcgggggc aaattgatgc tctggaacgg
    40021 tcgctggagg gtgatgccga atgccgtgcc atactccaac agatcgctgc cgttcggggc
    40081 gcggctaacg ggctgatggc agaagtgctt gaaagccata tccgggaaac gtttgaccga
    40141 aatgactgct acagccgcga agtcagccaa tccgttgacg acactattga actggttcgt
    40201 gcctatctta aatagctgaa tctattacca tattgaggaa gagcgagaga tgaaatcacg
    40261 tgctgccgtt gcatttgctc ccggtaaacc gctggaaatc gttgaaattg acgttgcacc
    40321 accgaaaaaa ggtgaagtgc tgattaaagt cacccatacc ggcgtttgcc ataccgacgc
    40381 atttaccctc tccggcgatg acccggaagg tgtattcccg gtagttctcg gtcacgaagg
    40441 ggccggcgtt gtggttgaag tcggtgaagg cgtaaccagc gtcaaacctg gcgaccatgt
    40501 gatcccgctt tacaccgcag agtgcggcga gtgtgagttc tgtcgttccg gcaaaaccaa
    40561 cctctgtgtt gcggttcgcg aaacccaggg taaaggcctg atgccagacg gcaccacccg
    40621 tttttcttac aacgggcagc cgctttatca ctacatggga tgctctacat tcagtgaata
    40681 caccgtggtc gcggaagtgt ctctggccaa aattaatcca gaggcaaacc acgaacacgt
    40741 ctgcctgctg ggctgtggcg tgaccaccgg tattggcgcg gtacacaaca cggctaaagt
    40801 ccagccagat gattctgttg ccgtatttgg tcttggcgcg attggtctgg cagtggttca
    40861 gggcgcgcgt caggcgaaag cgggacggat tatcgctatc gatacgaacc cgaagaaatt
    40921 cgatctggcg cgtcgcttcg gtgctaccga ctgcattaac ccgaatgact acgacaaacc
    40981 gattaaagat gtcctgctgg atatcaacaa atggggtatc gaccatacct ttgaatgcat
    41041 cggtaacgtc aacgtgatgc gtgcggcgct ggaaagtgcg caccgcggct ggggtcagtc
    41101 ggtgatcatc ggggtagctg gttctggtca ggaaatctcc acccgtccat tccagttggt
    41161 caccggtcgc gtatggaaag gttccgcgtt tggcggcgtg aaaggtcgtt cccagttacc
    41221 gggtatggtt gaagatgcga tgaaaggcga tatcgatctg gaaccgtttg tcacgcatac
    41281 catgagcctg gatgaaatta atgacgcctt tgacctgatg catgaaggca aatccattcg
    41341 aaccgtaatt cgttactgat ttcccgcagg tttacccgtc cagttcagac ggggttctta
    41401 atactctccc cgggcagccg tccgggggat taaccgtgag ataatgactg atggaactca
    41461 ttgaaaaaca tgccagcttt ggcggctggc aaaatgtgta tcggcattat tcccaatcac
    41521 tgaaatgtga aatgaatgtc ggcgtctatc tcccaccaaa agccgggaat gaaaaattac
    41581 cggtgctgta ctggctttcg ggcctgacct gcaacgagca gaatttcatt actaaatcgg
    41641 ggatgcagcg ttacgcggct gagcacaaca ttattgttgt tgcgccggac accagtccgc
    41701 gaggcagtca tgtcgcagat gccgaccgtt acgatctcgg gcaaggtgcc gggttttacc
    41761 tgaacgcaac gcaagcgccg tggaatgaac attacaaaat gtatgattat atccacaacg
    41821 agctgccgga tttagtgatg caacattttc cggcaacgac cagaaagtct atctcaggtc
    41881 attctatggg cggactgggc gcgctggtgc tggcgttacg taatccagat gaatatgtca
    41941 gcgtctcggc attttcgccc attgtctccc catcgcaagt accgtgggga cagcaagcct
    42001 ttgccgcata tcttggtgaa aataaagagg cctggttgga ttacgatccg gtgagcctta
    42061 tttcacaagg tcaacgcgtt gcggaaatca tggttgatca ggggttgagt gatgattttt
    42121 acgcagaaca gctgcggact ccaaatcttg aaaagatctg ccaggagatg aatatcaaga
    42181 cgttaatccg ttatcacgag ggttatgatc acagctatta ttttgtctcc agttttattg
    42241 gcgagcatat tgcgtaccac gccaataaac tgaatatgcg ttaataatag tgcacgactg
    42301 ccgga
//
LOCUS       DAMZFT010000025        42110 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.025, whole genome shotgun sequence.
ACCESSION   DAMZFT010000025 DAMZFT010000000
VERSION     DAMZFT010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 42110)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42110)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42110
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.025"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..1058
                     /gene="cueO"
                     /locus_tag="PN977_001501"
     CDS             <1..1058
                     /gene="cueO"
                     /locus_tag="PN977_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="multicopper oxidase CueO"
                     /protein_id="HDI6041548.1"
                     /translation="EILKLMLPKQWGIDDVPVIVQDKKFNADGQIDYQLDVMTAAVGW
                     FGDTLLTNGAIYPQHAAPRGWLRLRLLNGCNARSLNFATSDNRPLYVIASDGGLLPEP
                     VKVNELPVLMGERFEVLVEVNDNKPFDLVTLPVSQMGMAIAPFDKPHPVMRIQPIAIS
                     ASGALPDTLSSLPALPSLEGLTVRKLQLSMDPMLDMMGMQMLMEKYGDQAMAGMDHSQ
                     MMGHMGHGNMNHMNHGGKFDFHHANKINGQAFDMNKPMFAAAKGQYERWVISGVGDMM
                     LHPFHIHGTQFRILSENGKPPATHRAGWKDTVKVEGNVSEVLVKFNHDAPKEHAYMAH
                     CHLLEHEDTGMMLGFTV"
     gene            complement(1105..3495)
                     /gene="gcd"
                     /locus_tag="PN977_001502"
     CDS             complement(1105..3495)
                     /gene="gcd"
                     /locus_tag="PN977_001502"
                     /EC_number="1.1.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinoprotein glucose dehydrogenase"
                     /protein_id="HDI6041549.1"
                     /translation="MAINNTGSRRLLVTLTALFAALCGLYLLIGGGWLVAIGGSWYYP
                     IAGLVMLGVAWMLWRSKRAALWLYAALLLGTMIWGVWEVGFDFWALTPRSDILVFFGI
                     WLILPFVWRRLVIPASGAVAALVVALLISGGILTWAGFNDPQEINGTLSADATPAEAI
                     SPVADQDWPAYGRNQEGQRFSPLKQINADNVHNLKEAWVFRTGDVKQPNDPGEITNEV
                     TPIKVGDTLYLCTAHQRLFALDAASGKEKWHYDPELKTNESFQHVTCRGVSYHEAKAE
                     TASPEVMADCPRRIILPVNDGRLIAINAENGKLCETFANKGVLNLQSNMPDTKPGLYE
                     PTSPPIITDKTIVMAGSVTDNFSTRETSGVIRGFDVNTGELLWAFDPGAKDPNAIPSD
                     EHTFTFNSPNSWAPAAYDAKLDLVYLPMGVTTPDIWGGNRTPEQERYASSILALNATT
                     GKLAWSYQTVHHDLWDMDLPAQPTLADITVNGQKVPVIYAPAKTGNIFVLDRRNGELV
                     VPAPEKPVPQGAAKGDYVTPTQPFSELSFRPTKDLSGADMWGATMFDQLVCRVMFHQM
                     RYEGIFTPPSEQGTLVFPGNLGMFEWGGISVDPNREVAIANPMALPFVSKLIPRGPGN
                     PMEQPKDAKGTGTESGIQPQYGVPYGVTLNPFLSPFGLPCKQPAWGYISALDLKTNEV
                     VWKKRIGTPQDSMPFPMPVPVPFNMGMPMLGGPISTAGNVLFIAATADNYLRAYNMSN
                     GEKLWQGRLPAGGQATPMTYEVNGKQYVVISAGGHGSFGTKMGDYIVAYALPDDVK"
     gene            3701..4237
                     /gene="hpt"
                     /locus_tag="PN977_001503"
     CDS             3701..4237
                     /gene="hpt"
                     /locus_tag="PN977_001503"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004204384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HDI6041550.1"
                     /translation="MKHTVEVMIPEAEIKARIAELGRQITERYKDSGSDMVLVGLLRG
                     SFMFMADLCREVQVSHEVDFMTASSYGSGMSTTRDVKILKDLDEDIRGKDVLIVEDII
                     DSGNTLWKVREILSLREPKSLAICTLLDKPSRREVNVPVEFIGFSIPDEFVVGYGIDY
                     AQRYRHLPYIGKVILLDE"
     gene            complement(4278..4940)
                     /gene="can"
                     /locus_tag="PN977_001504"
     CDS             complement(4278..4940)
                     /gene="can"
                     /locus_tag="PN977_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonate dehydratase"
                     /protein_id="HDI6041551.1"
                     /translation="MKDIDTLISNNALWSKMLVEEDPGFFEKLAQAQKPRFLWIGCSD
                     SRVPAERLTGLEPGELFVHRNVANLVIHTDLNCLSVVQYAVDVLEVEHIIICGHYGCG
                     GVQAAVENPELGLINNWLLHIRDIWFKHSSLLGEMPQERRLDTLCELNVMEQVYNLGH
                     STIMQSAWKRGQKVTIHGWAYGIHDGLLRDLDVTATNRETLEQRYRHGISNLKLKHAN
                     HK"
     gene            5049..5975
                     /locus_tag="PN977_001505"
     CDS             5049..5975
                     /locus_tag="PN977_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDI6041552.1"
                     /translation="MTIALELQQLKKTYPGGVQALRGIDLQVEAGDFYALLGPNGAGK
                     STTIGIISSLVNKTSGRVSVFGYDLEKDVVNAKRQLGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVERKEAYIRSEKYLKQLDLWGKRNERARMLSGGMKRRLMIARALMHEPKLLIL
                     DEPTAGVDIELRRSMWGFLKDLNDKGTTIILTTHYLEEAEMLCRNIGIIQHGELVENT
                     SMKALLAKLKSETFILDLAPKSPLPKLDGYQYRLVDTATLEVEVLREQGINSVFTQLS
                     EQGIQVLSMRNKANRLEELFVSLVNEKQGDRA"
     gene            5972..6742
                     /locus_tag="PN977_001506"
     CDS             5972..6742
                     /locus_tag="PN977_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001747261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDI6041553.1"
                     /translation="MMHLYWVALKSIWAKEIHRFMRIWVQTLVPPVITMTLYFIIFGN
                     LIGSRIGDMHGFSYMQFIVPGLIMMSVITNAYANVASSFFGAKFQRNIEELLVAPVPT
                     HVIIAGYVGGGVARGLFVGILVTAISLFFVPFQVHSWVFVALTLVLTAVLFSLAGLLN
                     GVFAKTFDDISLVPTFVLTPLTYLGGVFYSLTLLPPFWQGLSHLNPIVYMISGFRYGF
                     LGINDVPLVTTFGVLVVFIVAFYLICWSLIQRGRGLRS"
     gene            6847..7287
                     /locus_tag="PN977_001507"
     CDS             6847..7287
                     /locus_tag="PN977_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDI6041554.1"
                     /translation="MLGWVITCHDDRAQEILDALEKKHGALLQCRAVNFWRGLSSNML
                     SRMMCDALHEADSGDGVIFLTDIAGAPPYRVASLLSHKHSRCEVISGVTLPLVEQMLA
                     YRETLSSAEFRERIVELGAPEVSSLWHQQQKNPPFVLKHNLYEY"
     gene            7351..8580
                     /locus_tag="PN977_001508"
     CDS             7351..8580
                     /locus_tag="PN977_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HDI6041555.1"
                     /translation="MYKQAVILLLMLFTASVSAALPARYMQTIENAAVWAQIGDKMVT
                     VGNIRAGQIIAVEPTAASYYAFNFGFGKGFIDKGHLEPVQGRQKVEDGLGDLNKPLSN
                     QNLITWKDTLVYNAPSVGSAPFGVLADNLRYPILHKLKDRLNQTWYQIRIGDRLAYIS
                     ALDAQPDNGLPVLTYHHILRDEENTRFRHTSTTTSVRAFNNQMAWLRDRGYATLSMAQ
                     LEGYVKNKINLPARAVVITFDDGLKSVSRYAYPVLKQYGMKATAFIVTSRIKRHPQKW
                     NPKSLQFMSVSELNEIRDVFDFQSHTHFLHRIDGYRRPILLSRSEHNILFDFARSRRA
                     LAQFNPHVLYLSYPFGGFNDKAVKAAKEAGFHLAVTTMKGKVKPGDNPLLLKRLYILR
                     TDSLETMSRLVSNQPQG"
     gene            complement(8584..8964)
                     /gene="panD"
                     /locus_tag="PN977_001509"
     CDS             complement(8584..8964)
                     /gene="panD"
                     /locus_tag="PN977_001509"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HDI6041556.1"
                     /translation="MIRTMLQGKLHRVKVTHADLHYEGSCAIDQDFLDAAGILENEAI
                     DIWNVTNGKRFSTYAIAAERGSRIISVNGAAAHCASVGDIVIIASFVTMPDEEARTWR
                     PNVAYFEGDNEMKRTAKAIPVQVA"
     gene            9238..10158
                     /locus_tag="PN977_001510"
     CDS             9238..10158
                     /locus_tag="PN977_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HDI6041557.1"
                     /translation="MDAPSTTPHDAVFKQFLMHAETARDFLDIHLPAELRELCDLDTL
                     HLESGSFIEESLKGHSTDVLYSVQMQGNTGYLHVVIEHQSKPDKKMAFRMMRYSIAAM
                     HRHLEADHDKLPLVVPILFYQGEATPYPLSMCWFDMFYSPELARRVYNSPFPLVDITI
                     TPDDEIMQHRRIAILELLQKHIRQRDLMLLLEQLVTLIDEGYTSGSQLVAMQNYMLQR
                     GHTEQADLFYGVLRDRETGGESMMTLAQWFEEKGRQEERQEVRQEVIQEVRQEVRQEF
                     ALRFLSKGMSREDVAEMANLPLAEVDKLIS"
     gene            10227..10442
                     /locus_tag="PN977_001511"
     CDS             10227..10442
                     /locus_tag="PN977_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000404120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDI6041558.1"
                     /translation="MEIKTRRETWQTLAQWFEEKGFQKGFQKGYKEGLQKVRLAQRLL
                     SKGMSREDVAEVTALSLAEIDKLINSN"
     gene            complement(10524..11375)
                     /gene="panC"
                     /locus_tag="PN977_001512"
     CDS             complement(10524..11375)
                     /gene="panC"
                     /locus_tag="PN977_001512"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HDI6041559.1"
                     /translation="MLIIETLPLLRQQIRRLRMEGKRVALVPTMGNLHDGHMKLVDEA
                     KARADVVVVSIFVNPMQFDRPEDLARYPRTLQEDCEKLNKRKVDLVFAPSVKEIYPNG
                     TETHTYVDVPGLSTMLEGASRPGHFRGVSTIVSKLFNLVQPDIACFGEKDFQQLALIR
                     KMVADMGFDIEIVGVPIMRAKDGLALSSRNGYLTAEQRKIAPGLYKVLSSIADKLQAG
                     ERDLDEIIAIAGQELNEKGFRSDDIQIRDADTLLEISENSKRAVILVAAWLGDARLID
                     NKIVELA"
     gene            complement(11387..12181)
                     /gene="panB"
                     /locus_tag="PN977_001513"
     CDS             complement(11387..12181)
                     /gene="panB"
                     /locus_tag="PN977_001513"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000805487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HDI6041560.1"
                     /translation="MKPTTIASLQKCKQDKKRFATITAYDYSFAKLFAEEGLNVMLVG
                     DSLGMTVQGHDSTLPVTVADIAYHTAAVRRGAPNCLLLADLPFMAYATPEQAFENAAT
                     VMRAGANMVKIEGGEWLVETVQMLTERAVPVCGHLGLTPQSVNIFGGYKVQGRGDEAG
                     DQLLSDALALEAAGAQLLVLECVPVELAKRITEALAIPVIGIGAGNVTDGQILVMHDA
                     FGITGGHIPKFAKNFLAETGDIRAAVRQYMAEVESGVYPGEEHSFH"
     gene            complement(12293..13564)
                     /locus_tag="PN977_001514"
     CDS             complement(12293..13564)
                     /locus_tag="PN977_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000848455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial-like adhesin"
                     /protein_id="HDI6041561.1"
                     /translation="MKTFFRYFLFLILCLSCYTASAGTDDFVGYVVGNNYGVGPSDQK
                     WRETGPNGDVTVVFRYGTSTNNLVFYKPTQLGPTGVKLHWSQLDTASGGGFLYCNRSN
                     NTSGGAMRIENAMVDSGKRYGNHKLFNTSVPGLYYTILISNMWSAYGTVTNVSSPGIY
                     IGDSAEQYFSWYNPSESILYQSCNNANTSSKYWAVGGIYQNLTIEFYTDTNFDPTVTQ
                     QVSLSRSSNYLYSFKAYGAGIGINEHSYFLRVDFDLLNIKLSNPTCFTAMLSGTSVTG
                     STVKMGEYSEAQIRNGATPVPFDISLQNCVRVTNIETKLVSTKVGTENRQLLGNTLTG
                     NGAAKGVGVLIEGLANSKSALMILKPNDSTSVYKDNTGQTQNNDSDAIYPEDDGITYP
                     LHFQATLKQDGNIAIEPGEFKATSTFQVTYP"
     gene            complement(13618..14214)
                     /locus_tag="PN977_001515"
     CDS             complement(13618..14214)
                     /locus_tag="PN977_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000953150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HDI6041562.1"
                     /translation="MLSSQLKIKIKTSLFLALLFATSSPVYAGLDVDMVANIKNSTCK
                     SGLSNLGNIDLGVVGVDYFSGNVAPEDDYPGGREFTITVTDCAIQGSGNVLNQLHINF
                     KPLNGVMATGSQQIFANENVAGAQNVGVVIFSTQDPANIFNVLTPAGSSRSIYPVMTA
                     DLNNSSWKFYTRMQKINPVLNVMPGQIISHVLIDIYYE"
     gene            complement(14241..14846)
                     /gene="yadL"
                     /locus_tag="PN977_001516"
     CDS             complement(14241..14846)
                     /gene="yadL"
                     /locus_tag="PN977_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000837848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial-like protein YadL"
                     /protein_id="HDI6041563.1"
                     /translation="MKSKTLCYGLPAGVFFAASLFTSQLFAATDSVKLNVKATVETGT
                     CTASLVDDNDQPISVIAFGDAYIPEIDAKTKIKIFKLQFKDCAGIPNKKALVKLTKRA
                     TCEGAANDGPGFANGSAAADKANAVAVEVWNTTTPGTDGKTQFSCVTPVAQEVNISTA
                     TGTTSVYHPMSARLVVEKNKSKTDITAGPFSAPATFSITYN"
     gene            complement(14861..15427)
                     /locus_tag="PN977_001517"
     CDS             complement(14861..15427)
                     /locus_tag="PN977_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001361645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HDI6041564.1"
                     /translation="MRITLHKLAILTGLFLSSSVLATDINVDFTATVKASTCNITLSG
                     DNVTPDGSDTYTLTFPGTIGLDKIANKTTQAQANFNLVASGCTTGVSKINTRLSGNAS
                     GTTPALIVPLSSDTTSTTNYIGMGIKALGEDDSTFLIPNSAPSFIPWEISDTDKTLEL
                     TVALRETTAGSGVPGDFRAQATFNFIYE"
     gene            complement(15447..18047)
                     /locus_tag="PN977_001518"
     CDS             complement(15447..18047)
                     /locus_tag="PN977_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000153160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane usher protein"
                     /protein_id="HDI6041565.1"
                     /translation="MSRKIIKTHNNKLTYIASFCALLLSPCAISAEHVEYDNTFLMGQ
                     DAFNIDLSRYTEGNPTLPGVYDVSVYINDQPVINQSISFITLEGKKNAQACITLKNLL
                     QFHINKPDINAENSILLQREGELGDCLDLANIIPQASVHYDVNDQRLDINVPQAWVMK
                     NYQNYVDSSLWENGINAAMLAYNVNAYHSEIPDRKNDSVYAAFNGGINLGAWRLRATG
                     NYNWMTNVGSDYDFQNRYLQRDLASLRSQLIVGESYTTGETFDAVSIRGIRLYSDSRM
                     LPPALASFAPIIHGVANTNAKVTITQGGYKIYETTVPPGAFVIDDLSPSGYGSDLIIT
                     IEESDGIKRTFSQPFSSVIQMQRPGVGRWDISAGQVLKDDIQNEPNLFQASYYYGLNN
                     YLTGYTGIQITDNNYTAGLLGLGLNTAYGAFSVDVTHSDVQIPDDKTYRGQSYRISWN
                     KLFEDTRTSLNIAAYRYSTQNYLGLNDALTLIDEVKHPEQDLEPKNMRNYSRMKNQVT
                     VSINQPLKFEKKDYGSFYLAGSWSDYWADGQNNTNYSIGYSNSASWGSYSISAQRSWS
                     QDGGNEDSIYLSFSIPIEKLLGSEHRDSGFQSIDTQLNSDFNGSNQLSISSSGYSTEN
                     HISYSVNTGYSMMKSSDDLGYIGGYASYESPWGTLSSSVSASSDNSRQISFNTDGGFV
                     LHSGGLTFSNDSFSDSDTLAVVQAPGAKGARINYGNSTIDRWGYGVTNALSPYHENRI
                     ALDINGLENDVELKSTSAITVPRQGSVVFAGFETVQGQSAIMNIKRTDGKNIPFAADI
                     YDENGNIIGNVGQGGQAFVRGIEQQGNIRINWLDDGKPVTCLAHYQQSAAPEKIAQTI
                     ILNGISCQIQ"
     gene            complement(18082..18822)
                     /locus_tag="PN977_001519"
     CDS             complement(18082..18822)
                     /locus_tag="PN977_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
                     /protein_id="HDI6041566.1"
                     /translation="MFLNAKHTAAVCFATCMSLSASAIADIVISGTRVIYKSNEKSVN
                     VRLENKGNNPLLVQSWLDTGDDSAEPGSITVPFTATPPVSRIDAKRGQTIKLMYTASS
                     PLPKDRESVFWFNVLEVPPKPDADKVANQSLLQLAFRTRIKLFYRPEGLNGLPSDAPA
                     ALKWSWATSAGKTALRVANPTPYHVSFSSGDLVANGKTYPLDVKMVAPLSEETFDIKG
                     MSGKASSAKVHFFAINDFGGAIEGNASL"
     gene            complement(18920..19504)
                     /locus_tag="PN977_001520"
     CDS             complement(18920..19504)
                     /locus_tag="PN977_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HDI6041567.1"
                     /translation="MSKKLGFALSGLMLAMVAGNASAALDGGQLNISGLVVDNTCETR
                     VDGGNKDGLVLLQTATVAEISAGVLNDTVGAKAKPFSITVDCSNANPNPGSTAKMTFG
                     SVFFGNSKGTLNNDMSINTPSDGVNIALHNIDGSTIKQVQINNPGDIYTKTLDATTKS
                     ATYDFKASYVRAEASQAPTAGYVKTNTAYTITYQ"
     gene            complement(19875..20354)
                     /gene="folK"
                     /locus_tag="PN977_001521"
     CDS             complement(19875..20354)
                     /gene="folK"
                     /locus_tag="PN977_001521"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HDI6041568.1"
                     /translation="MTVAYIAIGSNLASPLEQVNAALKALGDIPESRILAVSSFYRTP
                     PLGPQDQPDYLNAAVALETTLAPEELLNHTQRIELQQGRVRKAERWGPRTLDLDIMLF
                     GNEVINTERLTVPHYDMKNRGFMLWPLFEIAPELVFPDGETLRQILHTRAFEKLSKW"
     gene            complement(20351..21769)
                     /gene="pcnB"
                     /locus_tag="PN977_001522"
     CDS             complement(20351..21769)
                     /gene="pcnB"
                     /locus_tag="PN977_001522"
                     /EC_number="2.7.7.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000174652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HDI6041569.1"
                     /translation="MTLPRCTIFTRVANFCRKVLSREESEAEQAVARPQVTVIPREQH
                     AISRKDISENALKVMYRLNKAGYEAWLVGGGVRDLLLGKKPKDFDVTTNATPEQVRKL
                     FRNCRLVGRRFRLAHVMFGPEIIEVATFRGHHEGNVSDRTTSQRGQNGMLLRDNIFGS
                     IEEDAQRRDFTINSLYYSVADFTVRDYVGGMKDLKDGVIRLIGNPETRYREDPVRMLR
                     AVRFAAKLGMRISPETAEPIPRLATLLNDIPPARLFEESLKLLQAGYGYETYKLLCEY
                     HLFQPLFPTITRYFTENGDSPMERIIEQVLKNTDTRIHNDMRVNPAFLFAAMFWYPLL
                     ETAQKIAQESGLTYHDAFALAMNDVLDEACRSLAIPKRLTTLTRDIWQLQLRMSRRQG
                     KRAWKLLEHPKFRAAYDLLALRAEVERNAELQRLVKWWGEFQVSAPPDQKGMLNELDE
                     EPSPRRRTRRPRKRAPRREGTA"
     gene            complement(21808..22734)
                     /gene="gluQRS"
                     /locus_tag="PN977_001523"
     CDS             complement(21808..22734)
                     /gene="gluQRS"
                     /locus_tag="PN977_001523"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001432381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HDI6041570.1"
                     /translation="MLPPYFLFKEMTDTHYIGRFAPSPSGELHFGSLIAALGSYLQAR
                     ARQGRWLVRIEDIDPPREVPGAAETILRQLEHYGLHWDGDVLWQSQRHHAYREALAWL
                     YEQGLSYYCTCTRARIQSIGGIYDGHCRDLHHGPDNAAVRIRQLHPVTQFTDQLRGII
                     HADEKLAQEDFIIHRRDGLFAYNLAVVVDDHFQGVTEIVRGADLIEPTVRQISLYQLF
                     GWKVPDYIHLPLALNTQGAKLSKQNHAPALPKGDPRPVLIAALHFLGQQVETHWQDFS
                     VEQILQSAVKNWTLTAVPESAIVNSTFSNASC"
     gene            complement(22771..23226)
                     /gene="dksA"
                     /locus_tag="PN977_001524"
     CDS             complement(22771..23226)
                     /gene="dksA"
                     /locus_tag="PN977_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002209354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HDI6041571.1"
                     /translation="MQEGQNRKTSSLSILAIAGVEPYQEKPGEEYMNEAQLAHFRRIL
                     EAWRNQLRDEVDRTVTHMQDEAANFPDPVDRAAQEEEFSLELRNRDRERKLIKKIEKT
                     LKKVEDEDFGYCESCGVEIGIRRLEARPTADLCIDCKTLAEIREKQMAG"
     gene            complement(23404..24108)
                     /gene="sfsA"
                     /locus_tag="PN977_001525"
     CDS             complement(23404..24108)
                     /gene="sfsA"
                     /locus_tag="PN977_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HDI6041572.1"
                     /translation="MEFSPPLQRATLIQRYKRFLADVITPDGRELTLHCPNTGAMTGC
                     ATPGDTVWYSTSDNTKRKYPHTWELTQSQSGAFICVNTLWANRLTKEAILNESISELS
                     GYSSLKSEVKYGAERSRIDFMLQADSRPDCYIEVKSVTLAENEQGYFPDAVTERGQKH
                     LRELMSVAAEGQRAVIFFAVLHSAITRFSPARHIDEKYAQLLSEAQQRGVEILAYKAE
                     LSAEGMALKKSLPVTL"
     gene            complement(24123..24653)
                     /gene="thpR"
                     /locus_tag="PN977_001526"
     CDS             complement(24123..24653)
                     /gene="thpR"
                     /locus_tag="PN977_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HDI6041573.1"
                     /translation="MSEPQRLFFAIDLPAEIREQIIHWRATQFPPEAGRPVAADNLHL
                     TLAFLGEVSAEKEKALSLLAGRIRQPGFTLTLDDAGQWLRSRVVWLGMRQPPRGLIQL
                     ANMLRSQAARSGCFQSNRPFHPHITLLRDASEAVTIPPPGFNWSYTVTEFTLYASSFA
                     RGRTRYTPLKRWALTQ"
     gene            24727..27156
                     /gene="hrpB"
                     /locus_tag="PN977_001527"
     CDS             24727..27156
                     /gene="hrpB"
                     /locus_tag="PN977_001527"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308179.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase HrpB"
                     /protein_id="HDI6041574.1"
                     /translation="MSSLPVAAVLPELLAALDGASQVLLSAPTGAGKSTWLPLQLLAH
                     PGINGKVILLEPRRLAARNVAQRLAELLNEKPGDTVGYRMRAQNCVGPNTRLEVVTEG
                     VLTRMIQRDPELSGVGLVILDEFHERSLQADLALALLLDVQQGLRDDLKLLIMSATLD
                     NDRLQQMLPEAPVIISEGRSFPVERRYLPLPAHQRFDEAVAVATAEMLRQESGSLLLF
                     LPGVGEIQRVQEQLASRIGSDVLLCPLYGALSLNDQRKAILPAPQGMRKVVLATNIAE
                     TSLTIEGIRLVVDCAQERVARFDPRTGLTRLVTQRVSQASMTQRAGRAGRLDPGICLH
                     LIAKEQAERAAAQSEPEILQSDLSCLLMELLQWGCSDPAQMSWLDQPPTVNLLAAKRL
                     LRMLGALDGERLSAQGQKMAALGNDPRLAAMLVSAKSDDEAATAAKIAAILEEPPRMG
                     NSDLGVAFSRNQPAWQQRSQQLLKRLNVRGGEADSSLIAPLLARAYADRIARRRGQDE
                     RYQLANGMGAMLDADDALSRHEWLIAPLLLQGSASPDARILLALPVDIDELVQRCPQL
                     VQQSDTVEWDDAQGTLKAWRRLQIGQLMVKVQPLAKPSEDELHQAMLNGIRDKGLSVL
                     NWTAEAEQLRLRLLCAAKWLPEYDWPAVDDESLLATLETWLLPHMTGVHSLRGLKSLD
                     IYQALRGLLDWVMQQRLDSELPAHYTVPTGSRIAIRYHEDNPPALAVRMQEMFGAATN
                     PTIAQGRVPLVLELLSPAQRPLQITRDLSAFWKGAYREVQKEMKGRYPKHVWPDDPAN
                     TAPTRRTKKYS"
     gene            27250..29784
                     /gene="mrcB"
                     /locus_tag="PN977_001528"
     CDS             27250..29784
                     /gene="mrcB"
                     /locus_tag="PN977_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glycosyl
                     transferase/transpeptidase"
                     /protein_id="HDI6041575.1"
                     /translation="MAGNDREPIGRKGKPTRPVKQKVSRRRYEDDDDYDDYDDYEDEE
                     PMPRKGKGKGKGRKPRGKRGWLWLLLKLAIVFAVLIAIYGVYLDQKIRSRIDGKVWQL
                     PAAVYGRMVNLEPDMTISKNEMVNLLEATQYRQVSKMTRPGEFTVQANSIEMIRRPFD
                     FPDSKEGQVRARLTFDGDHLATIVNMENNRQFGFFRLDPRLITMISSPNGEQRLFVPR
                     SGFPDLLVDTLLATEDRHFYEHDGISLYSIGRAVLANLTAGRTVQGASTLTQQLVKNL
                     FLSSERSYWRKANEAYMALIMDARYSKDRILELYMNEVYLGQSGDNEIRGFPLASLYY
                     FGRPVEELSLDQQALLVGMVKGASIYNPWRNPKLALERRNLVLRLLQQQQIIDQELYD
                     MLSARPLGVQPRGGVISPQPAFMQLVRQELQAKLGDKVKDLSGVKIFTTFDSVAQDAA
                     EKAAVEGIPALKKQRKLSDLETAIVVVDRFSGEVRAMVGGSEPQFAGYNRAMQARRSI
                     GSLAKPATYLTALSQPKIYRLNTWIADAPIALRQPNGQVWSPQNDDRRYSESGRVMLV
                     DALTRSMNVPTVNLGMALGLPAVTETWIKLGVPKDQLHPVPAMLLGALNLTPIEVAQA
                     FQTIASGGNRAPLSALRSVIAEDGKVLYQSFPQAERAVPAQAAYLTLWTMQQVVQRGT
                     GRQLGAKYPNLHLAGKTGTTNNNVDTWFAGIDGSTVTITWVGRDNNQPTKLYGASGAM
                     SIYQRYLANQTPTPLNLVPPEDIADMGVDYDGNFVCSGGMRVLPVWTSDPQSLCQQSE
                     MQQQPSGNPFDQSSQPQQQPQQQPAQQEQKDSDGVAGWIKDMFGSN"
     gene            30004..32262
                     /gene="fhuA"
                     /locus_tag="PN977_001529"
     CDS             30004..32262
                     /gene="fhuA"
                     /locus_tag="PN977_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrichrome porin FhuA"
                     /protein_id="HDI6041576.1"
                     /translation="MARSKTAQPKHSLRKIAVVVATAVSGMSVYAQAAVEPKEDTITV
                     TAAPAPQESAWGPAATIAARQSATGTKTDTPIQKVPQSISVVTAEEMALHQPKSVKEA
                     LSYTPGVAVGTRGASNTYDYLIIRGFAADGQSQNNYLNGLKMQGNFYNDAVIDPYMLE
                     RAEIMRGPVSVLYGKSSPGGLLNMVSKRPTTEPLKEIQFKAGTDSLFQTGFDFSDALD
                     DDGVYSYRLTGIARSANAQQKGAEEQRYAIAPAFTWRPDDKTNFTFLSYFQNEPETGY
                     YGWLPKEGTVEPLPNGKRLPTDFNEGAKNNTYSRNEKMVGYSFDHEFNDTFTVRQNLR
                     FAQNKVSQKSVYGYGMCSDPLYTKDDDALKASPCLSIPQSEWNHTLTRQYVIDNEKLE
                     NFSVDTQLQSKFATGSVEHTLLTGVDFMRMRNDIDSWFGYAGSVAPSDIYNLDRSDFD
                     FGAHPDPSGPYRVLLKQKQTGLYVQDQAQWDKVLVTLGGRYDWAEQSSFNRDYGNKSD
                     RDDKQFTWRGGVNYLFDNGVTPYFSYSESFEPASLTDANGDLFAPSKGKQYEVGVKYV
                     PEDRPIVLTGALYQLTKTNNLMADPNNPNFSIEGGEIRARGVELEAKAALSASVNVVG
                     SYTYTDAEYTTDTTYKGNTPAQVPKHMASLWADYTFFDGPLSGLTLGTGGRYTGSSYG
                     DPANSFKVGSYTVVDALVRYDLARVGMAGSNVALHVNNLFDREYVASCFNTYGCFWGA
                     ERQVVATATFRF"
     gene            32313..33110
                     /gene="fhuC"
                     /locus_tag="PN977_001530"
     CDS             32313..33110
                     /gene="fhuC"
                     /locus_tag="PN977_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe3+-hydroxamate ABC transporter ATP-binding
                     protein FhuC"
                     /protein_id="HDI6041577.1"
                     /translation="MQEYTNHSDTTFALRNISFRVPGRTLLHPLSLTFPAGKVTGLIG
                     HNGSGKSTLLKMLGRHQPPSEGEILLDAQPLESWSSKAFARKVAYLPQQLPPAEGMTV
                     RELVAIGRYPWHGALGRFGAADREKVEEAISLVGLKPLAHRLVDSLSGGERQRAWIAM
                     LVAQDSRCLLLDEPTSALDIAHQVDVLALVHRLSQERGLTVIAVLHDINMAARYCDYL
                     VALRGGEMIAQGTPAEIMRGETLEMIYGIPMGILPHPAGAAPVSFVY"
     gene            33110..34000
                     /gene="fhuD"
                     /locus_tag="PN977_001531"
     CDS             33110..34000
                     /gene="fhuD"
                     /locus_tag="PN977_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter
                     substrate-binding protein FhuD"
                     /protein_id="HDI6041578.1"
                     /translation="MSGLPLISRRRLLTAMALSPLLWQMNTAHAAAIDPNRIVALEWL
                     PVELLLALGIVPYGVADTINYRLWVSEPPLPDSVIDVGLRTEPNLELLTEMKPSFMVW
                     SAGYGPSPEMLARIAPGRGFNFSDGKQPLAMARKSLTEMADLLNLQSAAETHLAHYED
                     FIRSMKPRFVKRGARPLLLTTLIDPRHMLVFGPNSLFQEILDEYGIPNAWQGETNFWG
                     STAVSIDRLAAYKDVDVLCFDHDNSKDMDTLMATPLWQAMPFVRAGRFQRVPAVWFYG
                     ATLSAMHFVRILDNAIGGKA"
     gene            33997..35979
                     /gene="fhuB"
                     /locus_tag="PN977_001532"
     CDS             33997..35979
                     /gene="fhuB"
                     /locus_tag="PN977_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter permease
                     FhuB"
                     /protein_id="HDI6041579.1"
                     /translation="MSKRIALFPVLLLVLLVVAAAALTWMNFSQALPRSQWAQAAWSP
                     DIDVIEQMIFHYSLLPRLAISLLVGAGLGLVGVLFQQVLRNPLAEPTTLGVATGAQLG
                     ITVTTLWAIPGAMASQFAALAGACVVGLIVFGVAWGKRLSPVTLILAGLVVSLYCGAI
                     NQLLVIFHHDQLQSMFLWSTGTLTQTDWGGVERLWPQLLGGVMLTLLLLRPLTLMGLD
                     DGVARNLGLALSLARLAALSLAIVISALLVNAVGIIGFIGLFAPLLAKMLGARRLLPR
                     LMLASLIGALILWLSDQIILWLTRVWMEVSTGSVTALIGAPLLLWLLPRLRSISAPDM
                     KVNDRVETERQHVLAFALAGGVLLLMAVVVALSFGRDAHGWTWASGALLDDLMPWRWP
                     RIMAALFAGIMLAVAGCIIQRLTGNPMASPEVLGISSGAAFGVVLMLFLVPGNAFGWL
                     LPAGSLGAAVTLLIIMIAAGRGGFSPHRMLLAGMALSTAFTMLLMMLQASGDPRMAQV
                     LTWISGSTYNATEAQVWRTGIVMVILLAITPLCRRWLTILPLGGDTARAVGMALTPTR
                     IALLLLAACLTATATMTIGPLSFVGLMAPHIARMMGFRRTMPHIVISALVGGLLLVFA
                     DWCGRMVLFPFQIPAGLLSTFIGAPYFIYLLRKQSR"
     gene            complement(36014..37294)
                     /gene="hemL"
                     /locus_tag="PN977_001533"
     CDS             complement(36014..37294)
                     /gene="hemL"
                     /locus_tag="PN977_001533"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HDI6041580.1"
                     /translation="MSKSENLYSAARELIPGGVNSPVRAFTGVGGTPLFIEKADGAYL
                     YDVDGKAYIDYVGSWGPMVLGHNHPAIRNAVIEAAERGLSFGAPTEMEVKMAQLVTEL
                     VPTMDMVRMVNSGTEATMSAIRLARGFTGRDKIIKFEGCYHGHADCLLVKAGSGALTL
                     GQPNSPGVPADFAKHTLTCTYNDLASVRAAFEQYPQEIACIIVEPVAGNMNCVPPLPE
                     FLPGLRALCDEFGALLIIDEVMTGFRVALAGAQDYYGVVPDLTCLGKIIGGGMPVGAF
                     GGRRDVMDALAPTGPVYQAGTLSGNPIAMAAGFACLNEVAQPGVHETLDELTTRLAEG
                     LLEAAEEAGIPLVVNHVGGMFGIFFTDAESVTCYQDVMACDVERFKRFFHMMLDEGVY
                     LAPSAFEAGFMSVAHSMEDINNTIDAARRVFAKL"
     gene            37519..38940
                     /gene="clcA"
                     /locus_tag="PN977_001534"
     CDS             37519..38940
                     /gene="clcA"
                     /locus_tag="PN977_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="H(+)/Cl(-) exchange transporter ClcA"
                     /protein_id="HDI6041581.1"
                     /translation="MKTDTPSLETPQAARLRRRQLIRQLLERDKTPLAILFMAAVVGT
                     LVGLAAVAFDKGVAWLQNQRMGALVHTADNYPLLLTVAFLCSAVLAMFGYFLVRKYAP
                     EAGGSGIPEIEGALEDQRPVRWWRVLPVKFFGGLGTLGGGMVLGREGPTVQIGGNIGR
                     MVLDVFRLKGDEARHTLLATGAAAGLAAAFNAPLAGILFIIEEMRPQFRYTLISIKAV
                     FIGVIMSTIMYRIFNHEVALIDVGKLSDAPLNTLWLYLILGIIFGIFGPIFNKWVLGM
                     QDLLHRVHGGNITKWVLMGGAIGGLCGLLGFVAPATSGGGFNLIPIATAGNFSMGMLV
                     FIFVARVITTLLCFSSGAPGGIFAPMLALGTVLGTAFGMVAVELFPQYHLEAGTFAIA
                     GMGALLAASIRAPLTGIILVLEMTDNYQLILPMIITGLGATLLAQFTGGKPLYSAILA
                     RTLAKQEAEQLARSKAASASENT"
     gene            38964..39029
                     /locus_tag="PN977_001535"
     CDS             38964..39029
                     /locus_tag="PN977_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6041582.1"
                     /translation="MAIIIGLEFAQLPMSFGAKYE"
     gene            39022..39366
                     /gene="erpA"
                     /locus_tag="PN977_001536"
     CDS             39022..39366
                     /gene="erpA"
                     /locus_tag="PN977_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HDI6041583.1"
                     /translation="MSDDVALPLEFTDAAANKVKSLIADEDNPNLKLRVYITGGGCSG
                     FQYGFTFDDQVNEGDMTIEKQGVGLVVDPMSLQYLVGGSVDYTEGLEGSRFIVTNPNA
                     KSTCGCGSSFSI"
     gene            complement(39413..40036)
                     /locus_tag="PN977_001537"
     CDS             complement(39413..40036)
                     /locus_tag="PN977_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRIC cation channel family protein"
                     /protein_id="HDI6041584.1"
                     /translation="MLVYWLDIVGTAVFAISGVLLAGKLRMDPFGVLVLGVVTAVGGG
                     TIRDMALDHGPVFWVKDPTDLVVAMVTSMLTIVLVRQPRRLPKWMLPVLDAVGLAVFV
                     GIGVNKAFNAEAGPLIAVCMGVITGVGGGIIRDVLAREIPMILRTEIYATACIIGGIV
                     HATAYYTFSVPLETASMMGMVVTLLIRLAAIRWHLKLPTFALDENGR"
     gene            complement(40074..40874)
                     /gene="btuF"
                     /locus_tag="PN977_001538"
     CDS             complement(40074..40874)
                     /gene="btuF"
                     /locus_tag="PN977_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter substrate-binding
                     protein BtuF"
                     /protein_id="HDI6041585.1"
                     /translation="MAKSLFRALVALSFLAPLWLNAAPRVITLSPANTELAFAAGITP
                     VGVSSYSDYPPQAQKIEQVSTWQGMNLERIVALKPDLVIAWRGGNAERQVDQLASLGI
                     KVMWVDATSIEQIANALRQLAPWSPQPDKAEQAAQSLLDQYAQLKAQYADKPKKRVFL
                     QFGINPPFTSGKESIQNQVLEVCGGENIFKDSRVPWPQVSREQVLARSPQAIVITGGP
                     DQIPKIKQYWGEQLKIPVIPLTSDWFERASPRIILAAQQLCNALSQVD"
     gene            complement(40867..41565)
                     /gene="mtnN"
                     /locus_tag="PN977_001539"
     CDS             complement(40867..41565)
                     /gene="mtnN"
                     /locus_tag="PN977_001539"
                     /EC_number="3.2.2.16"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/S-adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HDI6041586.1"
                     /translation="MKIGIIGAMEEEVTLLRDKIENRQTISLGGCEIYTGQLNGTEVA
                     LLKSGIGKVAAALGATLLLEHCKPDVIINTGSAGGLAPTLKVGDIVVSDEARYHDADV
                     TAFGYEYGQLPGCPAGFKADDKLIAAAEACIAELNLNAVRGLIVSGDAFINGSVGLAK
                     IRHNFPQAIAVEMEATAIAHVCHNFNVPFVVVRAISDVADQQSHLSFDEFLAVAAKQS
                     SLMVESLVQKLAHG"
     gene            41649..>42110
                     /gene="dgt"
                     /locus_tag="PN977_001540"
     CDS             41649..>42110
                     /gene="dgt"
                     /locus_tag="PN977_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dNTP triphosphohydrolase"
                     /protein_id="HDI6041587.1"
                     /translation="MAQIDFRKKINWHRRYRSPQGVKTEHEILRIFESDRGRIINSPA
                     IRRLQQKTQVFPLERNAAVRTRLTHSMEVQQVGRYIAKEILSRLKELKLLEAYGLDEL
                     TGPFESIVEMSCLMHDIGNPPFGHFGEAAINDWFRQRLHPEDAESQPLTDDR"
BASE COUNT        10018 a        10266 c        11162 g        10664 t
ORIGIN      
        1 acgagatcct gaaattaatg ctgccaaaac agtggggtat tgatgatgtc ccggtgattg
       61 ttcaggataa aaaatttaac gctgacgggc agattgatta tcaactggat gtgatgaccg
      121 ccgccgtggg ctggtttggt gatacgttgc tgaccaacgg cgctatctac ccgcaacacg
      181 ctgccccgcg tggctggctt cgcctgcgtt tgctcaatgg ctgtaatgcc cgctcgctta
      241 atttcgccac cagcgacaat cgcccgctgt atgtgattgc cagcgacggt ggtctgctac
      301 ctgaaccggt gaaggtgaac gagctgccag tgctgatggg cgagcgtttt gaagtgctgg
      361 tggaagttaa cgacaacaaa ccctttgacc tggtgacgct gccggtcagc cagatgggga
      421 tggcgattgc gccgtttgac aagccgcatc cggtaatgcg gattcagccg attgccatta
      481 gtgcctctgg tgctttgcca gacacattaa gtagcctgcc tgcgttacct tcgctggaag
      541 ggctgacggt acgcaagctg caactttcta tggacccaat gctcgatatg atggggatgc
      601 agatgctgat ggagaaatat ggcgatcagg cgatggccgg gatggatcac agccagatga
      661 tgggccatat ggggcacggc aatatgaatc atatgaacca cggcgggaag ttcgatttcc
      721 accatgccaa taaaatcaac ggtcaggcgt ttgatatgaa caagccgatg tttgcggcgg
      781 cgaaagggca atacgaacgt tgggttatct ctggcgtggg cgacatgatg ctgcatccgt
      841 tccatatcca cggtacgcag ttccgtatct tgtcagaaaa tggcaaaccg ccagcgactc
      901 atcgcgcagg ctggaaagat accgttaagg tagaaggtaa tgtcagcgaa gtgctggtga
      961 agtttaatca cgatgcaccg aaagaacatg cttatatggc gcactgccat ctgctggagc
     1021 atgaagatac ggggatgatg ttagggttta cggtataaaa agaccgtatt cggaaatatg
     1081 cccggatctc tccgggcatt atggttactt cacatcatcc ggcagcgcat aagccacaat
     1141 atagtcgccc atcttcgtac caaatgaacc gtgaccgcct gcggagatca ccacatactg
     1201 cttgccattc acttcatagg tcattggcgt agcctgacca cccgctggta aacggccctg
     1261 ccacagtttt tcaccgttgc tcatgttgta agcgcgcagg tagttatctg ccgtagcggc
     1321 gataaacagt acgttacccg ctgtggagat tggcccgccc agcatcggca tacccatatt
     1381 gaacggcacc ggtacaggca tcgggaatgg catactgtcc tgcggcgtac caatacgttt
     1441 cttccacacc acttcattgg ttttcagatc caatgccgag atatagcccc atgctggctg
     1501 tttacatggc agaccaaacg gtgagaggaa cgggttgagc gtgacaccat acggtacacc
     1561 gtactgtggc tgaatgccgg attccgtacc cgtgcctttg gcatctttcg gctgttccat
     1621 cgggttacca ggaccgcgtg ggatcagttt cgaaacaaac ggcagtgcca ttgggttggc
     1681 aatcgccact tcacgattcg gatcaacgga aatcccgccc cattcgaaca tccccaggtt
     1741 acccgggaag accagcgtac cctgttcaga tggcggggtg aaaatgcctt catagcgcat
     1801 ctggtggaac atcacgcggc acaccagctg gtcaaacatg gtggctcccc acatatccgc
     1861 accgctcaaa tctttcgtcg gacggaagct cagttcagaa aacggttgag ttggggttac
     1921 gtaatcgcct ttggcagcac cttggggaac cggtttttcc ggtgccggaa ccaccagttc
     1981 gccattacga cgatcgagca caaaaatgtt gccggttttc gccggagcat aaatgactgg
     2041 cactttctga ccattaacgg tgatgtccgc cagcgtcggc tgtgccggaa gatccatatc
     2101 ccacaggtcg tggtgaacgg tctggtagct ccacgccagt ttcccggtag tggcattcag
     2161 cgccagaatc gagctggcat aacgttcctg ttccggtgta cggttaccgc cccagatatc
     2221 cggcgtggtc acgcccatcg gcagatacac cagatccagc ttcgcgtcat aggccgctgg
     2281 tgcccacgag tttggtgagt taaaggtaaa ggtgtgttcg tcagacggga ttgcgttcgg
     2341 atctttcgcg cccggatcaa aagcccacag cagctccccg gtgttgacat caaaaccacg
     2401 gatcacgcca gacgtttcgc gggttgagaa gttatcggtg actgaaccgg ccatcacgat
     2461 ggttttatcg gtgataattg gcggcgaagt cggctcatac agacccggtt tggtgtccgg
     2521 catattgctt tgcagattga gcacgccttt attagcgaag gtttcgcaca gcttgccgtt
     2581 ttcagcgtta atcgcaatca ggcgaccatc attgaccgga agaatgatac gacgcgggca
     2641 atccgccatc acttctggcg aggccgtttc tgctttggct tcatgataag agacgccacg
     2701 acaggttacg tgctggaagg actcgttggt tttcagttca ggatcgtaat gccatttctc
     2761 tttgccgctg gcggcatcga gcgcaaacag gcgctggtga gcggtacata agtaaagcgt
     2821 atcgcccact ttaatcggcg tcacttcatt ggtgatttcg cccggatcgt tcggctgttt
     2881 cacatcgcct gtacggaaca cccaggcttc tttcaggttg tgaacgttat cggcgttaat
     2941 ttgcttcagt ggtgaaaagc gttgaccttc ctgattgcga ccatacgcag gccagtcctg
     3001 atcggcaacc ggtgagatag cttcggcagg tgtggcatcg gcgcttaagg tgccgttgat
     3061 ttcctgcgga tcgttaaatc ccgcccaggt gaggattccg ccactaatca gcaatgccac
     3121 caccagtgcg gcaactgcgc cgctggcagg aatgaccagg cgacgccaga caaacggcag
     3181 gatcagccag atgccgaaga agaccagaat gtcgctgcgc ggagtcagcg cccagaagtc
     3241 gaaaccaact tcccagacgc cccaaatcat ggtgccgagc agcagggcgg cgtacagcca
     3301 aagcgcggcg cgtttactgc gccacagcat ccaggcgacg ccgagcatca caaggccagc
     3361 gatagggtag taccaggagc cgccaatcgc gaccagccag cctccgccaa tgagtagata
     3421 cagcccgcaa agcgctgcaa aaagggctgt tagcgtgacg agtaatcgtc gcgagcctgt
     3481 attgttaatt gccataaaga gacaccattt caattcatta atattttagt agcaattaat
     3541 tataggtttt aacatgtgtg atcgtcatca caattcgagc tttattaaca gattccgcga
     3601 atgaatagtt ttactggtat actgcgtgtc ttgcgctttg ttgcggtgcc aaaacctgcc
     3661 cgtgcgaagt gatttgtttt taaatcatat ggttagagat atgaaacata ctgtagaagt
     3721 aatgatcccc gaagcggaga ttaaagcgcg tatcgccgaa ctgggtcgtc agattactga
     3781 gcgttacaaa gacagcggca gcgatatggt gctggtgggt ctgctgcgtg gctcatttat
     3841 gtttatggcg gacctgtgcc gtgaagttca ggtatctcat gaagtcgatt ttatgaccgc
     3901 ctccagctac ggtagcggca tgtccaccac ccgtgatgtg aaaatcctca aagatctgga
     3961 tgaagatatc cgtggcaagg acgtgctgat tgttgaagat atcatcgatt cgggtaatac
     4021 actgtggaaa gtgcgtgaga tcttaagcct gcgcgaaccg aagtcgctgg cgatttgtac
     4081 gctgctggat aaaccgtccc gtcgtgaagt gaacgtcccg gtagaattta tcggtttctc
     4141 gatcccggat gagtttgtgg tgggttacgg cattgattac gcacagcgtt accgtcatct
     4201 gccgtatatc ggcaaagtga ttctgctgga cgagtaagtg tgaagttgcc ggatgtgttg
     4261 catccggcat ggcattttta tttgtggttg gcgtgtttca gcttgaggtt ggagatcccg
     4321 tgacggtaac gttgctcaag ggtttcgcgg ttagtcgctg tgacatccag atcacgcagc
     4381 aagccgtcgt gaatgccgta ggcccagccg tgaatggtga ctttctgccc gcgtttccac
     4441 gctgattgca taatagtgga gtggcccagg ttatacacct gttccatgac gttcagttca
     4501 cacaaggtat ccagacggcg ctcttgcggc atttcgccga gcaatgagct atgtttgaac
     4561 cagatatcgc ggatatgcag cagccagttg ttgataagcc ccagttccgg gttttcaact
     4621 gcggcttgta cgccgccgca accgtagtgg ccacagataa taatgtgttc aacttcgagt
     4681 acatccactg catactgaac cacggaaagg cagttcaggt cggtgtgaat gaccaggtta
     4741 gcaacattac ggtgaacaaa gagttcgccc ggctcaagac cggttaaacg ttctgcagga
     4801 acgcgactgt cggaacatcc aatccataga aagcgcggtt tttgcgcttg tgccagtttc
     4861 tcaaaaaacc cgggatcctc ttccaccagc atttttgacc atagtgcatt gttgctgatg
     4921 agtgtatcta tgtctttcat ggaggttaac gacctgtaac caaataatta cgtttggcta
     4981 atatagggca actccgggac gatttaaacc acagataaag tgtaagaacg taaggtaagt
     5041 aaaaatttat gaccattgca ctggaacttc aacagcttaa aaaaacctat ccaggcggcg
     5101 ttcaggcgct tcgtggaatc gatttgcagg tcgaagcggg tgatttttat gcgcttctcg
     5161 ggccgaacgg ggccggaaaa tcgaccacta tcggtattat cagctctctg gtaaataaaa
     5221 cctccgggcg ggtcagcgta tttggttacg atctcgagaa agatgtcgta aacgctaaac
     5281 gtcagttggg tctggtgcca caggaattta acttcaaccc gtttgaaacc gtgcagcaaa
     5341 ttgtggtgaa tcaggcaggg tactacggtg tggagcgcaa agaagcgtac attcgcagcg
     5401 aaaagtatct taaacaactc gatctatggg gaaaacgcaa cgaacgtgcg cgtatgttat
     5461 ctggcgggat gaagcgccgt ttaatgattg cccgtgcgtt aatgcatgaa cctaaactac
     5521 tgattctcga cgaaccgacc gcaggcgtgg atattgaact tcgccgctca atgtggggct
     5581 ttttgaagga tttaaacgac aaaggcacca ccatcattct caccacacac tacctggaag
     5641 aagcagaaat gctgtgccgc aatatcggca ttattcaaca cggtgagttg gtggaaaata
     5701 cttcgatgaa ggcgctgctg gcgaagctga aatcagaaac ctttattctc gatctcgcgc
     5761 cgaaaagtcc gttaccgaag ctcgatggct atcagtatcg actggtcgat accgcgacgc
     5821 tggaagttga agtgttgcgt gagcagggga taaacagcgt attcacgcag ttaagtgagc
     5881 agggcattca ggtattaagt atgcgtaaca aagctaaccg tctggaagag ctgtttgttt
     5941 cactggttaa tgaaaaacaa ggagatcgcg catgatgcat ctttactggg tggcgctaaa
     6001 aagcatctgg gcgaaagaga tccatcgctt tatgcgtatc tgggtgcaga cgctggtgcc
     6061 gccagtcatc accatgaccc tttactttat tatcttcggt aacctgattg gttcgcgtat
     6121 tggcgatatg catggcttca gctacatgca gttcatcgta ccggggctga tcatgatgtc
     6181 ggtgatcacc aacgcctacg ccaacgtcgc gtcatcattt tttggtgcca agttccagcg
     6241 taatattgaa gagctgctgg tagcgccggt tccgactcat gtcattattg ccggatatgt
     6301 cggtggtggc gtggcgcgtg gtttgtttgt tggtattttg gtgacggcaa tttcactgtt
     6361 ttttgtgccg tttcaggtgc attcgtgggt attcgttgcc ttaaccctgg tgctcactgc
     6421 ggtgctattc tcccttgcgg gtttgctgaa cggcgtgttt gccaaaacgt tcgatgacat
     6481 cagcctggtg ccaacctttg tgttaacgcc actcacgtat ttgggcgggg tcttttactc
     6541 cctgactttg ctgccgccgt tctggcaggg actgtcgcac ctgaacccga tcgtttatat
     6601 gatcagtggt ttccgctacg gcttcctcgg tatcaatgat gtgccgctgg tcactacctt
     6661 tggcgtactg gtggtcttta ttgtggcgtt ttatttgatt tgctggtcgc tgatccaacg
     6721 tgggcgtggt ctacgtagct aaggctattc cctctcctct ggattcgggg gagaggagtt
     6781 ttgacgacta tcacccttta ttaaccatgg tcagggtaga ctgattttcg gctaaggagg
     6841 aaggcgatgc taggttgggt tattacctgt cacgatgatc gggcgcaaga gatactggat
     6901 gcgctggaga aaaaacacgg ggcgcttctt cagtgccggg ccgtgaattt ctggcgcgga
     6961 ttaagttcta atatgctcag ccgtatgatg tgcgatgctc tgcatgaagc agactctggc
     7021 gatggggtga tctttttaac tgatattgcc ggagcgccgc cgtaccgcgt ggcttcgcta
     7081 ttaagccaca aacactcccg ctgtgaagtg atttctgggg taacgttacc gttagttgaa
     7141 cagatgctgg cctaccgtga aacattatcc agcgctgagt ttcgcgagcg aattgtcgaa
     7201 ctgggtgcgc cggaggtcag tagtctttgg caccaacaac aaaaaaatcc gcctttcgtc
     7261 ctcaaacata atttgtatga gtattaaccc gcgatcctga tgacgctttt gctacaataa
     7321 aagcgttgtt tcaccctcgg ttattttttc atgtacaaac aagctgttat tctcctgctg
     7381 atgctgttta ccgcaagcgt cagtgccgca ttacctgccc gttatatgca aaccatcgaa
     7441 aatgccgcgg tctgggcgca aattggcgac aaaatggtga cggtagggaa tattcgggcc
     7501 gggcaaatca ttgccgtgga gcccactgcc gcaagttatt acgcatttaa ttttggcttt
     7561 ggtaaagggt ttatcgataa aggccatctc gagccggttc aggggcgaca aaaagttgaa
     7621 gatggtttgg gtgacctcaa caagccgctg agtaatcaga acttaataac ctggaaagat
     7681 acactggttt ataacgcacc gagtgtgggc agtgcgccat ttggagtact ggcggacaat
     7741 ttgcgctacc cgattttgca taaactgaaa gacaggttaa atcaaacctg gtatcagatc
     7801 cgtattggcg atcgactggc ctatatcagc gcgctggatg cccaacccga taatggcctg
     7861 ccggtgctaa cctatcacca tattttgcgc gacgaagaaa acacccgttt tcgccatact
     7921 tcgacgacca cctcggtacg cgctttcaat aaccagatgg cctggctgcg cgacagggga
     7981 tacgcgacac tgagcatggc gcagttggaa ggttacgtga agaataagat caatctccct
     8041 gcgcgagcgg tggtaattac ctttgatgat ggcctcaagt cggtgagccg ctatgcgtat
     8101 cctgtgttga aacaatatgg tatgaaggcg acggcgttta ttgttacctc gcgcatcaaa
     8161 cgtcacccgc agaaatggaa cccaaaatcg ctgcaattta tgagcgtttc tgaacttaac
     8221 gaaattcgcg atgtatttga tttccagtca catactcatt ttttgcatcg gatagatggt
     8281 tatcggcggc ctatattgct gagccgtagt gagcacaata ttctgtttga ttttgcacgt
     8341 tcacgccgcg ctctggcgca atttaatcca catgttttgt atctttctta tccatttggc
     8401 ggatttaatg acaaagccgt gaaggcagca aaagaagccg gatttcacct ggcggtgacg
     8461 accatgaaag gcaaagtaaa accgggggat aatccgttgt tactaaaacg actttatatc
     8521 ttaagaacgg attcgctgga gacgatgtcg cggctggtga gtaaccagcc gcagggataa
     8581 caatcaggca acctgtaccg gaatcgcttt cgcggtacgt ttcatttcgt tgtcgccttc
     8641 aaaataggcg acgttgggtc gccaggtgcg agcttcttca tctggcatgg taacgaagct
     8701 ggcgatgatg acaatatcgc cgacgctggc gcagtgggcc gccgcaccgt taacggaaat
     8761 aattctcgaa ccgcgttctg ccgcgatggc ataggtggag aaacgcttgc cgttggtcac
     8821 attccagata tcaatggctt cgttttcgag aataccagct gcgtcaagaa aatcctggtc
     8881 aatggcgcaa gaaccttcat agtgcaggtc cgcatgagtc actttcacgc ggtggagttt
     8941 gccctgcagc atcgtgcgaa tcataacttc tacctttcta ccctgtcgtt aacgaagcag
     9001 gcgatgcctg ctttgaggaa attctcacgc agtattgccc gatttttgtt tagtgtctac
     9061 tcatctgacg gcatttgcat cagcagtttg cgaaccgtgc cgaagcgtgc catgtaatgc
     9121 ctgtaaccaa ttgaaattta tctgaacgct gctcggtaaa cactaaagag gcggctgacg
     9181 acggcgcgcc ttgactggat actgccttca cgcaagacaa cacagaaggc agggaagatg
     9241 gatgcaccga gtaccacacc gcatgatgcg gtgtttaaac aatttttaat gcatgcggaa
     9301 acggctcgtg actttctgga tatccatttg ccagcggaac tacgcgaact gtgtgacctc
     9361 gacacgctgc atcttgagtc ggggagtttt attgaagaaa gcctgaaagg gcacagcact
     9421 gacgtgctct attccgtgca aatgcagggt aatacgggct atctacatgt tgtaattgaa
     9481 caccaaagca agccggacaa aaaaatggcc tttcgcatga tgcgttattc tattgctgcc
     9541 atgcaccggc atctggaggc agatcacgat aagctgccgc tggtggtgcc gattttgttt
     9601 tatcagggcg aggccacgcc ttatccactc tcaatgtgct ggtttgatat gttttactcg
     9661 ccggagctgg cgcgacgcgt ctataacagt cctttcccgc tggtggatat cactatcaca
     9721 ccggatgacg aaatcatgca acatcggcgg attgcgattc tcgaactgct gcaaaaacat
     9781 attcgccaac gcgacttaat gttattgctg gagcaactgg tcacgctgat agacgaaggg
     9841 tacactagcg gaagtcagtt agttgccatg caaaactata tgctgcaacg cggtcatact
     9901 gaacaagcgg atttgtttta tggtgtgctg agagacaggg aaacgggagg ggagtctatg
     9961 atgacgctgg cgcagtggtt tgaagagaag ggaagacagg aggaaaggca ggaggtaaga
    10021 caggaggtaa tacaagaggt tagacaggaa gtaagacagg aattcgccct gcgttttctg
    10081 agtaaaggga tgtctcggga agacgttgca gagatggcaa atttacctct tgctgaggtt
    10141 gataagctga ttagctaagt tatctgcatt atcagttata gccagataat tgttattgtt
    10201 aactgaccag ataattaagg gaacatatgg aaataaagac acggagagaa acctggcaga
    10261 cgttggcgca gtggtttgaa gagaagggat ttcagaaggg atttcagaag ggatataaag
    10321 agggactcca gaaggtaaga ctggcccagc gtcttctgag taaaggaatg tctcgggaag
    10381 acgttgcaga ggtgacagct ttatctcttg cagagattga taagctaatt aactctaact
    10441 aagttgtcta cgatatgaat aattgcttac agatgacgtg gcaatgaata actggcgcca
    10501 tcagccgtag cgccagttaa atattacgcc agttctacga ttttgttgtc gatcaggcga
    10561 gcatcgccaa gccaggcggc taccaggatc accgctcgtt tgctgttttc agagatttcc
    10621 agcaaggtgt cggcatcgcg aatctgaata tcatcggaac ggaaaccttt ttcattcagt
    10681 tcttgcccgg caatagcaat aatttcatcg agatcccgtt cgccagcctg caatttgtcg
    10741 gcaattgaac ttaagacctt gtacagaccc ggcgcaattt tgcgttgttc cgccgtcaga
    10801 taaccgttac gggaactcag cgccagaccg tctttggcgc gcataatcgg tacgccgaca
    10861 atctcaatat caaagcccat atcagcaacc attttgcgga tcagcgccag ttgctgaaaa
    10921 tctttttcac cgaagcaagc gatatctggc tggaccaggt tgaaaagctt gctgacaata
    10981 gtcgaaacgc cgcgaaaatg tcctggacgg ctggcacctt ccagcatggt ggaaaggcct
    11041 ggaacatcga cataggtgtg ggtttctgta ccgttcggat agatctcttt taccgaggga
    11101 gcgaaaacca gatccacttt acgtttgttt agcttctcgc agtcttcctg caaggtgcgt
    11161 gggtagcgag ccagatcttc cgggcggtca aactgcatcg ggttaacgaa aatactgacg
    11221 acgaccacat cggcgcgggc tttggcttcg tcaaccagct tcatatggcc gtcgtgcagg
    11281 ttacccatgg tgggcaccag cgccacgcgc ttaccttcca tacgcaggcg gcggatttgc
    11341 tgacgcagca gcggcagggt ttcgataatt aacacaacgt cactccttaa tggaaactgt
    11401 gttcttcgcc cgggtaaacg ccggactcca cttcagccat atactgccgc acagccgcgc
    11461 ggatgtcgcc cgtttcggcg aggaaatttt tggcgaattt aggaatatga ccgccggtaa
    11521 tgccgaacgc gtcgtgcatc acgaggattt gcccgtcagt gacgttgcct gcgccaatac
    11581 cgataaccgg gatcgccagt gcttcggtaa tgcgttttgc cagctcaacc ggcacgcact
    11641 ccagtaccag cagctgtgcc ccagcagctt ctaatgccaa tgcatcgctg agcagttgat
    11701 ctcccgcttc atcgccgcgc ccctggactt tgtagccacc gaaaatattt actgactgtg
    11761 gggttaggcc taaatgacca caaactggaa cggcacgttc ggtcagcatt tgtacggttt
    11821 ccaccagcca ctcaccgcct tcaattttga ccatgttggc tccggcacgc attaccgttg
    11881 cggcgttttc aaaggcttgt tccggcgtgg catacgccat aaatggcagg tcagccagca
    11941 gaaggcagtt tggcgcgccg cgacgcacag cggcggtgtg ataggcgata tcggcaacgg
    12001 taaccggtag cgtggagtca tgcccctgaa ccgtcatgcc cagtgaatcg cctaccagca
    12061 tgacgttaag tccttcttcg gcaaacagtt ttgcgaagct atagtcgtaa gcggtgatgg
    12121 ttgcgaaacg ttttttgtcc tgcttgcatt tctgcaatga agcaatcgtg gttggtttca
    12181 tagcgtatcc tgataaataa tgttgtgctg tctcgcattt tatcagtcac attggtgggg
    12241 gcaatgattt atccgtatcg caccgccgtt gtctggcggt gcgatagtgt tatcaggggt
    12301 aggtaacctg aaaagtgctg gtggctttaa attcgccggg ttcaatagca atattcccgt
    12361 cttgttttaa tgtagcctgg aaatgaagtg gatacgttat accgtcatct tccgggtaaa
    12421 tcgcatctga atcgttattc tgcgtctggc ccgtattatc tttataaaca gaagttgaat
    12481 cattaggttt cagtatcatg agggcacttt tactgtttgc taatccttca atcaataccc
    12541 caacgccttt tgctgcgcca tttcctgtga gagtgttacc aaggagttgc ctgttttcag
    12601 taccaacctt tgttgaaact aattttgtct cgatattagt cacacgaacg cagttttgaa
    12661 gtgaaatatc aaaaggaacc ggtgtggcac cgtttcttat ttgtgcttca ctatactcgc
    12721 ccatttttac cgtagaacca gtaacagatg ttccacttaa catagctgta aaacaagtgg
    12781 gattacttaa tttaatattt agtaggtcaa aatcaactcg aagaaaatag ctgtgttcgt
    12841 taatgccgat accagcacca taagctttaa atgaatataa atagtttgat gagcgcgata
    12901 atgaaacttg ttgagttact gttggatcaa agttggtatc tgtataaaat tcgatggtaa
    12961 gattttgata aataccgcct acagcccaat atttgctgga tgtgttggca ttgttacaac
    13021 tttggtataa tatgctttcg cttggattgt accatgaaaa atattgttct gcagaatcac
    13081 caatatatat tcctggtgaa ctaacgttag ttacagtacc gtaagctgac cacatatttg
    13141 aaattaatat tgtataatac aaaccgggaa cagatgtatt gaatagttta tggttaccat
    13201 atctttttcc agagtcaacc attgcatttt cgatacgcat tgccccgcca ctggtgttat
    13261 tgcttctgtt acaataaaga aagcctccgc cagaagcagt atctaactga ctccaatgta
    13321 atttcacccc tgttggaccc agttgtgttg gtttataaaa tactaaatta tttgtactgg
    13381 taccatacct gaacacgact gtcacatctc catttggccc ggtttctctc catttttgat
    13441 ctgacggacc aacgccgtag ttattcccaa caacatatcc tacgaaatca tcagtgccag
    13501 cactcgcggt ataacaactc aagcacagaa ttaaaaaaag aaaatatctg aagaatgtct
    13561 tcattttcac tccaccatcc actgctgtaa tagtcgcagt aatttgtttt tggtatgtta
    13621 ctcgtagtaa atatcaatta atacatggct tatgatttgg ccgggcatga catttaatac
    13681 agggttgatt ttttgcatcc gtgtatagaa tttccaggat gaattgttta aatcagcagt
    13741 catcaccgga taaattgaac gagaactgcc cgctggggta agaacattaa atatattagc
    13801 cgggtcctga gtggaaaata ttactacacc tacgttttga gcgccggcga cattttcatt
    13861 tgcaaaaatt tgctgagagc ctgtcgccat cacgccatta agcggtttaa agttaatatg
    13921 taactgattt aacacatttc cagaaccctg gatggcgcag tcagtcacag tgatagtgaa
    13981 ctctctccca cccggataat catcttcagg tgcgacgtta ccagaaaaat aatccacacc
    14041 aaccacacca aggtcgatat taccaaggtt actgaggcca cttttacatg tactgttctt
    14101 gatattggca accatatcca catccagccc tgcatataca ggactggatg tggcaaacaa
    14161 taatgccagg aacagacttg tttttatctt aatttttagt tgagaggaaa gcataatcgg
    14221 tccctgactt taatggcctg ttagttatag gtgatagaaa atgttgcagg tgcggaaaac
    14281 ggaccggcag tgatgtcggt tttgctttta tttttctcta ccaccaggcg tgcactcata
    14341 ggatgatata ctgacgtagt tcctgttgca gtggagatat tcacttcctg agcgactggt
    14401 gttacgcagc taaattgagt cttgccgtct gtaccaggtg tcgtggtatt ccatacttca
    14461 accgcaacag catttgcttt atcagcagcg gcggagccgt ttgcaaaacc aggcccatcg
    14521 ttggctgctc cttcacaagt tgcgcgttta gttaatttta cgagtgcttt tttattagga
    14581 ataccagcac aatccttaaa ctgtaactta aatattttaa tttttgtttt ggcatcaatt
    14641 tcaggaatgt aagcatcccc aaatgcaata acagaaatag gctgatcatt gtcatccacc
    14701 aggctggcgg tacaggttcc cgtctctacg gttgctttaa cattcagctt aacgctatcg
    14761 gtagctgcaa acaattggct ggtgaatagg gatgccgcaa aaaaaacacc agcgggcagt
    14821 ccataacata atgttttaga tttcattatt atcatccctg ttattcataa atgaaattga
    14881 aagttgcctg ggcgcggaaa tcgccaggaa caccactgcc agccgtggtt tcgcgcagtg
    14941 caacggtcag ttcaagagtt ttgtctgtat cactaatttc ccatggaata aaactcggcg
    15001 cactgtttgg gattaagaat gtcgaatcat cttcaccaag tgcttttata cccataccaa
    15061 tataattagt tgttgatgta gtgtcagaag agagtggaac aattaatgcg ggagtagttc
    15121 cggaagcatt cccactgagc ctggtattga ttttgctgac gcctgtggta caaccgctgg
    15181 caaccagatt aaaattcgcc tgtgcttgag tcgttttatt tgcaattttg tccagaccaa
    15241 tagttcctgg gaaagttaat gtgtaggtgt cactcccatc aggggtgaca ttgtcgcctg
    15301 ataacgtaat gttacaagtt gatgctttta cggttgctgt aaaatcaaca ttaatgtctg
    15361 ttgcaagtac agaggatgat aaaaataatc cagtcagtat tgccaattta tgcagtgtta
    15421 ttctcattat gtgaaattcc ctgttgttac tgaatctgac aactaattcc attcagaata
    15481 atagtttgcg ctattttttc tggtgctgcg ctctgctggt aatgagcaag gcaagtgaca
    15541 ggtttaccgt cgtcaagcca gttgatacgt atgttacctt gttgttctat acctcgaaca
    15601 aaggcctgac caccttgccc tacattgcca ataatatttc cgttctcgtc ataaatatct
    15661 gcggcaaacg gaatgttttt accgtcagtt cgcttgatgt tcataatcgc tgattgtccc
    15721 tgaactgttt caaaaccagc aaagacaaca gacccttggc gggggacagt aatagcactg
    15781 gtacttttca attcaacgtc attttccaga ccgttaatat cgagtgcaat tcggttttca
    15841 tgataagggg agagcgcgtt agtaacacca tatccccaac gatctatcgt actattacca
    15901 tagttaatac gcgccccttt agctcctggc gcttgtacaa cggccagagt gtctgagtcg
    15961 ctaaagctgt cattactgaa ggtcaggccg ccgctatgta aaacaaatcc gccgtcggta
    16021 ttaaatgaga tttgacggct attatcactg cttgcggaaa ctgaactgga aagagttccc
    16081 cagggagatt catagcttgc atatccgccg atatagccca aatcatcact ggatttcatc
    16141 atcgaatagc ctgtattgac gctataactg atatggtttt cggtgctata accactactg
    16201 ctgatactga gttgattgct gccgttgaaa tcactattta actgagtatc aatgctctga
    16261 aaaccagagt cgcgatgttc tgaacccagt aacttttcga taggaatgct aaagcttaga
    16321 tagatgctgt cttcattacc gccgtcctgg ctccaggacc gttgggcact aatgctgtag
    16381 cttccccacg atgcgctgtt actataacca atggagtagt tagtattatt ttgtccgtcc
    16441 gcccaatagt cagaccaact tccggcaaga tagaatgagc catagtcttt cttttcaaac
    16501 ttgaggggtt ggttaatact aaccgtaacc tgatttttca tgcgtgaata gttacgcata
    16561 tttttaggtt ccaggtcctg ttccggatgt tttacttcat cgatcaatgt cagtgcatcg
    16621 ttaagcccca gataattctg ggttgaataa cggtaagcgg cgatattgag cgaggttctg
    16681 gtatcttcaa ataacttatt ccaggagatg cgataacttt gcccgcggta ggttttatcg
    16741 tccggaattt gcacatccga gtgggttaca tcgactgaaa acgcaccgta cgcggtattc
    16801 aggccaaggc ccaacagccc ggcagtatag ttattatcgg taatttgaat accggtataa
    16861 cctgtaagat agttgttcag accatagtag tagctggcct ggaacaaatt tggctcattt
    16921 tgaatatcgt cttttaatac ctgaccagca ctgatgtccc atcttccaac accagggcgt
    16981 tgcatctgaa ttaccgaaga aaatggctgg gaaaaagttc gttttatgcc atcagattct
    17041 tcgattgtga taatgagatc gctgccgtag cctgatgggc ttaaatcatc aataacaaat
    17101 gctcccggcg gtacagtagt ttcatatatt ttatacccgc cctgggtaat ggtgactttt
    17161 gcgttggtgt tagcgacacc atgaataata ggcgcaaaac tagctaacgc aggcggtagc
    17221 attcggctgt cgctgtataa acgaataccg cgaatactga cagcatcaaa ggtttcccca
    17281 gttgtgtatg actcacccac tattaactgt gaacgcaaag aggccaggtc gcgctgcaaa
    17341 tagcgattct gaaaatcgta atcactgcct acattggtca tccagttgta gttgccggtt
    17401 gcgcgaagtc gccatgcccc cagatttata ccgccgttaa atgcggcgta aacactgtcg
    17461 ttttttctgt ccggaatttc gctgtgatat gcgttgacgt tgtaggctag cattgcggcg
    17521 ttaataccgt tttcccacag tgaagagtca acatagtttt ggtaattctt cattacccag
    17581 gcttgaggaa cattgatgtc cagtcgttga tcgttgacgt cataatgaac cgaggcctga
    17641 ggaatgatat ttgctaaatc aagacaatct ccgagttcgc cttcgcgctg tagaaggatg
    17701 gaattctcgg catttatatc tggtttatta atgtgaaact gcaataaatt cttcagggtg
    17761 atacaagcct gcgcattctt cttaccttcg agggtaataa aggaaatgct ttggttgata
    17821 accggttgat cattgatata aacactgacg tcatacacgc cgggcaaggt agggttacct
    17881 tcggtatacc gactaaggtc gatgttaaaa gcatcttgcc ccatcaaaaa ggtattatca
    17941 tattcaacgt gttcagcgct tatggcacag gggcttaata gcaatgcgca aaacgaggct
    18001 atatatgtaa gtttgttatt atgcgttttt atgatttttc gggacacgtt gtgcttcctg
    18061 ctgcgtttat tcctgattta gttacaggct ggcattgcct tcaattgcac caccaaagtc
    18121 atttatggcg aagaaatgca ctttggcaga acttgcttta ccactcatgc cttttatatc
    18181 gaaggtttct tcactcagcg gggctaccat ttttacatcc agggggtagg ttttgccatt
    18241 ggcgactaaa tcaccactac tgaatgagac gtgatacggt gtcggatttg cgacgcgcaa
    18301 tgcagttttt ccagcagagg ttgcccatga ccatttcagt gctgcgggtg catctgatgg
    18361 cagtccgttt aaaccttccg ggcgataaaa taatttgata cgcgtacgga atgccaattg
    18421 cagaagactc tggttggcga ctttatctgc gtcaggtttg ggtggaacct cgagtacgtt
    18481 aaaccagaaa acactttctc ggtcttttgg cagcggtgag gaagccgtgt acatcaattt
    18541 aatagtttga ccacgtttgg catcaattcg cgaaacagga ggggtggcgg taaatgggac
    18601 agtaatactc cctggttcag cactatcgtc gccagtatcc agccagctct gaacgagtaa
    18661 cggattattc cccttatttt ccagccgaac attaacgctt ttttcattac ttttataaat
    18721 tacgcgagtt cctgaaatta cgatatctgc tattgccgat gcgcttaagc tcatacaagt
    18781 ggcgaagcat acagctgcag tatgtttagc gtttaaaaac ataaaccatt cccgacagag
    18841 ttaattaaaa tagggagtaa cattccacta ttaagaaagt tactcccata gatattcagt
    18901 ttatgaatct aacgaaggat tactggtaag taatggtata tgcagtgttg gttttaacat
    18961 aaccagcagt tggtgcctgg cttgcttcag cacggacata ggatgcttta aaatcatagg
    19021 ttgcagattt agtcgtagcg tccagcgttt ttgtgtaaat atcgcctggg ttgttaattt
    19081 gaacctgttt gatagtggaa ccatcaatat tgtgcagggc aatatttacg ccgtcagaag
    19141 gcgtgttaat agacatatcg ttgttcagag tccctttgct gttgccaaag aacacggaac
    19201 cgaaggtcat ttttgcggtg ctaccaggat tcgggtttgc attgctgcag tcaacggtga
    19261 tgctgaaagg tttagcttta gcacctacgg tgtcattcag tacgccagca ctgatttcag
    19321 caacagttgc agtttgcaga agcaccaggc catctttgtt gccaccatca acacgggttt
    19381 cacaggtgtt atcaactacc agaccactga tatttaattg gccaccgtcc agtgcagcag
    19441 aagcattacc tgcaaccatt gccagcataa gaccagacag ggcaaaacct aattttttag
    19501 acataaaaat ccttgaataa aattccatta gtcagactac atgtttgaag agtaactatt
    19561 catgacacga atataagaaa caaatgttag atattaatga gcaatgatgt ttgttaccaa
    19621 aatttacaac cattgttcat taggttgcct attgtgcact ttagaagctt ttgaacaaat
    19681 taaatttact taattcaaga ttaagtaaat ttaagttcac aagtgcaatt agttagggta
    19741 tggcgatggg attgatattt ctcagcggga atttatatta tgtttctata aaaaattttt
    19801 aaactaaaat cgttattaag gaaagagagt cggtgtgatt ttcagaaaat atttatattc
    19861 gtaatataaa taaattacca tttgcttaat ttttcaaatg ctcttgtatg taagatttga
    19921 cgcaacgtct ccccatcagg aaacactaac tccggcgcaa tttcaaacag tggccacagc
    19981 ataaagccac gatttttcat atcgtagtgc ggaacggtca gacgttcggt atttattact
    20041 tcattaccaa acagcatgat gtcgagatcc agcgtgcgcg gtccccagcg ttcagcttta
    20101 cggacgcgac cttgctgcaa ttcgatacgt tgtgtgtgat tgagtagctc ttcaggggca
    20161 agagtggttt ccagcgcgac ggctgcattt aagtaatcgg gttgatcttg cggccccagc
    20221 ggtggggtgc ggtaaaacga agaaacagca agaatgcggc tttctggaat atcgcctaat
    20281 gctttcagag cagcattgac ctgctccagc ggagaggcca gattgctgcc tatggcaata
    20341 tacgccactg tcatgcggta ccctcacgac gcggtgcgcg tttgcgtgga cgacgagtac
    20401 gacgacgcgg tgacggttct tcatccagtt cgttaagcat ccctttttgg tctggcggcg
    20461 cggaaacctg gaactcaccc caccatttca ccagacgttg cagttcagcg ttacgctcaa
    20521 cttcggctcg taaggccaac aggtcgtatg ccgcacggaa cttaggatgc tccagcagtt
    20581 tccatgcgcg ttttccctga cgacgggaca tacgcaactg caactgccag atatcgcgag
    20641 ttagcgtagt cagacgtttc gggatcgcca gtgaacggca ggcttcgtcc agcacgtcgt
    20701 tcatcgccag tgcgaaagcg tcgtgatagg tcaggccgct ttcctgggcg atcttctgtg
    20761 ccgtctccag caatgggtac cagaacatgg cggcaaacag gaacgccggg ttcacgcgca
    20821 tatcgttatg gatacgcgta tcggtattct tcagcacctg ttcaatgatc cgctccatcg
    20881 ggctgtcgcc attttccgtg aagtagcggg taatggtcgg aaacagcggc tggaacagat
    20941 gatattcaca cagcagctta taagtttcgt aaccgtagcc cgcttgcaac agtttcagcg
    21001 attcttcaaa cagacgtgcc ggtgggatat cgttcagtaa ggttgccagg cgtgggatcg
    21061 gctcggcagt ttccgggctg atgcgcatgc ccaatttggc ggcaaaacga accgcgcgaa
    21121 gcatccgtac cggatcttca cggtagcgcg tttccgggtt accaatcaga cggataacgc
    21181 cgtctttcag atccttcatg ccgccaacgt aatcacggac ggtaaaatct gccacgctgt
    21241 aatacaggct gttgatagtg aaatcgcggc gctgggcgtc ttcttcgatg gagccgaaaa
    21301 tgttgtcgcg cagcaacata ccgttttgcc cgcgttggga ggtcgtgcga tcgctgacgt
    21361 taccttcgtg atgtccacgg aaggtcgcaa cttcgataat ctccgggcca aacatcacat
    21421 gagccagacg gaaacggcga cccaccaggc ggcagttacg gaacagtttg cgcacctgct
    21481 ctggcgtggc gttggtggtc acgtcaaaat ctttcggctt tttgccaagt aacaggtcgc
    21541 gcacgccgcc gccaaccagc caggcttcgt atcccgcttt attgagcctg tacattacct
    21601 tcagggcatt ttcactgata tctttgcggg aaatagcatg ctgctcacgc gggatcaccg
    21661 tcacctgtgg acgggcgact gcctgttcag cctcgctttc ctcgcggctt agcaccttgc
    21721 ggcaaaaatt agcgactcgg gtaaaaatag tacacctcgg tagtgtcaaa catcattcag
    21781 gacaaaaaaa tagcggctaa tcatagctca gcatgacgca tttgagaatg ttgaatttac
    21841 aattgccgac tcaggcacgg cagtcagcgt ccagtttttg acggctgact gaaggatttg
    21901 ctcgacgctg aaatcctgcc agtgtgtttc tacctgctga cccagaaaat gaagtgccgc
    21961 gattaacacc gggcgcggat cgcctttcgg caacgcaggc gcatgattct gcttggaaag
    22021 tttagcgcct tgtgtgttaa gcgccagcgg taaatgaatg taatctggca ctttccagcc
    22081 aaaaagctgg tacagcgaga tttgtcgcac tgtcggttca atcaaatctg ccccacgaac
    22141 aatttctgtg acgccctgga agtgatcatc gaccaccacc gccaggttat aggcaaacaa
    22201 accatcacga cgatgaataa taaaatcttc ttgcgccagt ttttcgtcgg cgtgaataat
    22261 tccgcgtagt tgatcagtaa actgcgtgac cggatgcaac tgccgaattc gcacagccgc
    22321 gttgtctggt ccatgatgca aatcccggca atgaccgtcg taaataccgc caatgctttg
    22381 aatacgcgca cgcgtgcagg tgcagtaata acttagtcct tgctcatata accaggcgag
    22441 tgcttcgcga taggcgtggt gacgttgcga ttgccagaga acgtcgccgt cccagtgcag
    22501 gccgtaatgt tccagctggc gcaggatagt ctctgcggca ccgggaactt cacgaggcgg
    22561 gtcgatatct tctatgcgta ccagccagcg accttgccgg gcgcgagcct gcaaatagct
    22621 gccaagcgcg gcaatcagag aaccaaaatg aagctcgccg gaaggagagg gggcgaagcg
    22681 gccaatatag tgtgtgtctg tcatctcttt gaacaaaaaa taaggcggga gcatttcccg
    22741 cctgtggtaa acgtgatgga acggctgtaa ttagccagcc atctgttttt cgcgaatttc
    22801 agccagcgtt ttgcagtcga tgcacagatc ggctgtcggg cgcgcttcca gacggcgaat
    22861 accaatttca acaccgcagg attcgcagta gccgaaatct tcgtcttcca cttttttcag
    22921 cgtcttctcg atctttttga tcagcttacg ctcgcgatcg cggttacgca gttcgaggct
    22981 gaactcttct tcctgggctg cacggtctac cgggtccggg aagttggctg cttcatcctg
    23041 catatgtgta acggtgcgat cgacttcatc cctgagttga ttacgccatg cttccagaat
    23101 acgacggaag tgcgccagct gggcttcatt catatactct tcgcccggct tctcctgata
    23161 tggctccacc ccagcgatgg cgagaatact cagggacgat gttttacggt tttgcccttc
    23221 ttgcatgttg cttctcctta acacgcacta tcgatcccca tgttcggggg aaaaatgagg
    23281 ccgctataaa tagcagatgc ttttccggat agcaattatc taaacgtaac acttgacaac
    23341 tgtgtgagga aaagcgtatt tgcgcacgcg accagaatgt aaattaacca gttacttact
    23401 ctactacaat gtaaccggta gtgatttttt aagagccatg ccttcggcag aaagttccgc
    23461 tttgtaagcc agaatttcta cccccctctg ttgagcttct gacaatagtt gcgcgtattt
    23521 ctcatcgatg tggcgcgcgg gtgaaaaccg tgtaatggct gaatgcagca cggcgaaaaa
    23581 gataaccgca cgctggcctt cagccgctac gctcatcaac tcccgaaggt gtttctgacc
    23641 tcgttcagtg accgcatcgg gaaaatatcc ctgttcgttc tccgctaacg taaccgattt
    23701 cacttcaata tagcagtctg gacgcgaatc cgcctgcaac ataaagtcaa tacggctgcg
    23761 ttctgcgccg tattttactt cgcttttcag cgagctatag cctgacagtt ctgaaattga
    23821 ttcattaagg atagcctctt tcgtcagcct gttagcccaa agtgtattga cgcaaataaa
    23881 tgcgccgctc tggctttgag ttaattccca ggtgtgtggg tatttccgtt tggtgttgtc
    23941 tgaagttgaa taccagacgg tatcgccagg cgttgcacaa ccggtcatcg cacccgtatt
    24001 cgggcagtgt agcgttaatt cgcgaccatc gggtgtgatc acatcggcta aaaaacgttt
    24061 gtaacgctga attagcgtcg cgcgctgtag agggggagaa aattccattg caacaatcct
    24121 tgttattgcg ttagcgccca gcgttttagc ggcgtgtagc gtgtgcgtcc acgggcaaac
    24181 gaggaggcgt aaagggtgaa ctccgtcacc gtatacgacc agttaaagcc tggcggcggg
    24241 attgtcaccg cctcgctggc gtcgcgcaat aaggtgatat gtggatgaaa cggtcgattg
    24301 ctttgaaaac aaccgctgcg tgcagcttgt gaacgaagca tattcgccag ctggattaag
    24361 ccgcgtggcg gctgtcgcat ccctaaccac accacacgcg aacgcagcca ttgaccggcg
    24421 tcatcaagcg tgagtgtgaa accaggttga cgaatccgtc cggctaaaag agaaagcgcc
    24481 ttctctttct ctgcgctcac ttcgcctaaa aatgccagag tcagatgcaa attatcggcg
    24541 gcgaccggac gtcccgcctc aggtggaaac tgtgtggcgc gccagtggat aatctgttcg
    24601 cggatttctg caggtaagtc gatagcaaag aacagccgtt gcggttcaga catgtgaggc
    24661 actcggttat gaattaccgc gatgctacaa tgtggcgcga agaatgttaa ccctctggag
    24721 cgttttgtgt cgtcgttgcc cgttgctgcc gtcttacctg agttacttgc tgccctcgat
    24781 ggtgcatcgc aggtgttatt aagtgcgccg accggggccg ggaaatcaac ctggctgccg
    24841 ctgcaactgc tggcgcatcc aggcattaac gggaaagtta tcctgctgga gccgcgtcgt
    24901 ctggcggcgc gtaacgttgc gcaacggctg gcggagctgc ttaacgaaaa gccaggcgat
    24961 accgttggct accggatgcg tgcgcaaaac tgcgtcgggc cgaatacccg cctggaagtg
    25021 gttaccgaag gcgtgctgac gcgcatgatc cagcgtgacc cggaactgag cggtgttgga
    25081 ttggtgatcc tcgatgagtt tcatgagcgc agtttgcagg cggatttggc gttggcgctg
    25141 ttactcgatg tgcaacaagg tctgcgtgat gaccttaaac tgctgattat gtcggctacg
    25201 ctggacaacg accgcttgca gcaaatgctg ccagaagcgc ccgtcatcat ctcagaaggg
    25261 cgctcgtttc cggttgaacg ccgttattta ccactgcccg cgcatcagcg ttttgacgaa
    25321 gccgttgcgg tagccaccgc cgaaatgctg cgtcaggaaa gcggatcatt actgttattt
    25381 ttacctggcg tcggagaaat tcagcgtgtg caggaacaac tggcttcgcg catcggcagt
    25441 gatgtgttgc tctgcccgct gtatggcgcg ttgtcgctga acgatcagcg aaaagcgatc
    25501 ctcccggcac cgcaagggat gcgcaaagtg gtgctggcga ccaatattgc tgaaaccagt
    25561 ttaaccatcg aaggtattcg tctggtggtg gattgtgctc aggaacgtgt ggcgcgtttt
    25621 gatccgcgta cagggcttac gcggctggtt actcaacgcg ttagtcaggc gtcgatgacg
    25681 cagcgtgccg ggcgtgccgg gcgtctggat ccgggtatct gcctgcattt aatcgccaaa
    25741 gaacaagcag aacgcgccgc ggcgcaaagt gaaccggaaa tattacaaag cgatctttcc
    25801 tgtttgctga tggaattact gcaatgggga tgcagcgatc cggcgcagat gagctggctg
    25861 gatcaaccgc caacggtgaa tctactggcc gcgaaacgcc tgttacggat gctgggggcg
    25921 ctggacggtg aacggcttag tgcgcaaggg caaaaaatgg cagcgctggg gaacgatccg
    25981 cgtttagcgg caatgctggt gagcgcgaag agcgacgacg aagctgctac cgcggcaaaa
    26041 attgccgcca ttctcgaaga gccgccacgg atgggcaata gtgacctggg cgtggcgttt
    26101 tcgcgcaatc aacccgcctg gcagcaacgt agtcagcaac tgttaaaacg cttaaacgta
    26161 cgcggcggtg aggcagacag ttcgcttatc gcgccgctac ttgccagagc gtatgccgat
    26221 cgcattgctc gtcgccgtgg gcaagatgaa cgctatcaac tggcgaacgg catgggggcg
    26281 atgctcgatg ccgacgatgc gctaagccgc cacgaatggt tgatcgcacc gttattattg
    26341 cagggcagcg cctcgccgga tgcgcggatt ttactggcgc taccggtcga tattgatgag
    26401 ttagtacaac gctgcccgca gctggtacag caatcggaca ccgtggagtg ggatgacgcg
    26461 caaggtacgc tgaaagcctg gcgtcggctg caaatcggtc agttgatggt gaaagtgcag
    26521 ccactggcga aaccctcgga agacgagttg catcaggcga tgcttaacgg cattcgtgat
    26581 aaaggtttaa gcgtgctcaa ctggacggcg gaagcggaac agctacgctt gcgtttgtta
    26641 tgcgccgcaa agtggttgcc ggaatatgac tggccagcgg ttgatgatga gagtttgttg
    26701 gcgacgctgg aaacgtggct actgccgcat atgacaggcg tacattcgct acgcggcttg
    26761 aaatcactcg atatttatca ggcactgcgt ggattacttg attgggtaat gcagcaacgt
    26821 ctggatagtg aattgcctgc gcattacact gtgccgacgg gaagccggat cgccattcgt
    26881 tatcatgaag ataacccgcc cgcgctggcg gtgagaatgc aggagatgtt tggcgcggcc
    26941 accaatccga cgatcgccca ggggcgcgtg ccgctggtgc tggagttact ttcccctgcc
    27001 caaaggccgc tgcaaatcac gcgtgatttg agcgccttct ggaaaggagc gtaccgtgag
    27061 gtgcaaaaag agatgaaagg gcgttatccc aaacatgtct ggccggacga cccggcaaac
    27121 accgcaccaa cgcgacgaac gaaaaagtat tcgtgaagat caaagtaaat ttgagagata
    27181 tcttcttctg tcttgcaaca gaagaacaga aaatcgggct tttgcgcctg aatattgcgg
    27241 agaaaaagca tggccgggaa tgaccgcgag ccaattggac gcaaagggaa accgacgcgt
    27301 ccggtcaaac aaaaggtaag ccgtcgtcgt tacgaagatg acgatgatta cgacgattat
    27361 gatgactatg aggatgaaga accgatgccg cgcaaaggta agggcaaagg caaagggcgt
    27421 aagcctcgtg gcaaacgcgg ctggctatgg ctgctgctaa aactggctat cgtttttgcc
    27481 gtgctgatcg ccatttacgg cgtttatctc gatcaaaaaa ttcgcagccg tattgatggc
    27541 aaggtctggc aactgcccgc ggcagtttat ggccggatgg tcaaccttga gccagacatg
    27601 accatcagca agaacgagat ggtgaatctg ctggaggcga cccagtatcg tcaggtgtcg
    27661 aaaatgaccc gtcctggcga atttaccgtg caggccaaca gcattgagat gatccgccgt
    27721 ccgtttgatt tcccggacag taaagaaggg caggtgcgtg cacgtctgac ctttgatggc
    27781 gatcatctgg cgacgatcgt caatatggag aacaaccgtc agttcggttt cttccgtctt
    27841 gatccgcgcc tgatcactat gatctcttca ccaaacggtg agcagcgtct gtttgtgccg
    27901 cgcagtggtt tcccggattt gctggtggat actttgctgg cgacagaaga ccgtcatttc
    27961 tacgagcatg atgggatcag tctctactca atcggacgtg cggtgctggc aaacctgact
    28021 gccggacgca cggtgcaggg ggcgagtacg ctgacgcaac agttggttaa aaacctgttc
    28081 ctctccagcg agcgttctta ctggcgtaaa gcgaacgaag cctacatggc gctgatcatg
    28141 gacgcgcgtt acagcaaaga ccgtattctt gagctgtata tgaacgaggt gtatctcggt
    28201 cagagcggcg acaacgaaat ccgcggcttc ccgctggcga gcttgtatta ctttggtcgc
    28261 ccggtagaag aattgagcct cgatcagcag gcgctgttgg tgggtatggt gaaaggggcg
    28321 tccatctaca acccgtggcg taacccgaaa ctggcgctgg agcgacgtaa cctggtactg
    28381 cgtctgctgc aacagcaaca gattattgat caagaactct atgacatgtt gagtgcacgt
    28441 ccgctgggtg ttcagccgcg cggtggggtg atctctcctc agccagcctt tatgcaactg
    28501 gtgcgccagg agcttcaggc taaattaggc gataaggtaa aagatctctc cggcgtgaag
    28561 atcttcacta cctttgactc ggtggcccag gacgcggcag aaaaagccgc cgtggaaggc
    28621 attccggcac tgaaaaaaca gcgtaagttg agcgatcttg aaaccgcgat tgtggttgtt
    28681 gaccgcttta gcggtgaagt tcgtgcgatg gtcggtggtt ctgagccgca gtttgcgggc
    28741 tacaaccgtg cgatgcaggc gcgtcgttcg attggttccc tcgcaaaacc agcgacttat
    28801 ctgactgcct taagccagcc gaaaatctat cgcctgaaca cgtggattgc cgatgcgccc
    28861 atcgcgctgc gtcagccgaa tggccaggtc tggtcaccgc agaacgatga ccgtcgttat
    28921 agcgaaagtg gcagagtgat gctggtggat gcgttgaccc gttcgatgaa cgtgccgacg
    28981 gtgaatctgg ggatggcgct ggggctgcct gcggttacgg agacctggat taaactgggc
    29041 gtgccgaaag atcagctgca tccggttccg gcgatgctgc tgggggcgtt gaacttaacg
    29101 ccaatcgaag tggcgcaggc attccagact atcgccagcg gcggtaaccg tgcaccgctt
    29161 tctgcgctgc gttcggtaat tgcggaagat ggcaaagtgc tgtatcagag cttcccgcag
    29221 gcggaacgcg cggttccggc gcaggcggcg tatctgacac tatggaccat gcagcaggtg
    29281 gtacaacgtg gtacggggcg tcagcttggg gcgaaatacc cgaacctgca tctggcaggg
    29341 aaaacaggaa ctaccaacaa taacgtagat acctggtttg cgggcattga cggcagcacg
    29401 gtgaccatca cctgggtagg ccgtgataac aaccaaccga ccaaactgta tggagccagc
    29461 ggggcaatgt cgatttatca gcgttatctg gctaaccaga cgccaacacc gctgaatctt
    29521 gttccgccgg aagatattgc agatatgggc gtggactacg acggcaactt tgtttgcagc
    29581 ggtggtatgc gtgtcttgcc ggtctggacc agcgatccgc aatcgctgtg ccagcagagt
    29641 gagatgcagc agcagccgtc aggcaatccg tttgatcagt cttctcagcc gcagcaacag
    29701 ccgcaacagc aacctgctca gcaagagcag aaagacagcg acggtgtagc cggttggatc
    29761 aaggatatgt ttggtagtaa ttaacgtcta agcgtgacat accggatggc gagtagccat
    29821 ccggtaaaat aacatcccac ctaaagtatt aaccctttct tttcatctgg ttgtttatta
    29881 acccttcagg aacgctcaga ttgcgtaccg cttgcgaacc cgccagcgtt tcgaatatta
    29941 tcttatcttt ataataatca ttctcgttta cgttatcatt cactttacat cagagatata
    30001 ccaatggcgc gttccaaaac tgctcagcca aaacactcac tgcgtaaaat cgcagttgta
    30061 gtagccacag cggttagcgg catgtctgtt tatgcacagg cagcggttga accgaaagaa
    30121 gacactatca ccgttaccgc tgcacctgcg ccgcaagaaa gcgcatgggg gccagctgca
    30181 actattgcgg cgcgacagtc cgctaccggc actaaaaccg atacgccgat tcaaaaagtg
    30241 ccacagtcta tttctgttgt gaccgccgaa gagatggcgc tgcatcagcc gaagtcggta
    30301 aaagaagctc ttagctacac tcctggcgtt gccgtgggaa ctcgtggcgc atccaacact
    30361 tacgattacc tgatcatccg cggatttgcc gctgacggcc aaagccagaa caactatctg
    30421 aatggcctga agatgcaggg caacttctat aacgatgcag ttatcgatcc gtatatgctg
    30481 gagcgcgctg aaatcatgcg tggtccggta tccgtgctgt acgggaaaag cagccctggc
    30541 ggcctgttga acatggtcag caagcgcccg actacagaac cgctgaaaga aattcagttt
    30601 aaagccggta ctgacagcct gttccagact ggttttgact tcagtgatgc gctggatgat
    30661 gacggcgttt actcttatcg tctgactggt attgcgcgtt cagccaatgc tcagcagaag
    30721 ggggcagaag agcagcgtta tgctattgct ccagcgttca cctggcgccc ggatgataaa
    30781 accaatttca ctttcctttc ttacttccag aacgaaccgg aaaccggtta ttacggctgg
    30841 ttgccgaaag agggaaccgt tgagccgctg ccgaacggta agcgtctgcc gacagacttt
    30901 aatgaagggg cgaagaacaa cacctattct cgtaatgaga agatggtggg ctacagcttc
    30961 gatcacgaat ttaacgacac ctttactgtg cgtcagaacc tgcgctttgc gcaaaataaa
    31021 gtctcgcaaa agagcgtata tggctacggc atgtgctcgg atccgctgta taccaaagac
    31081 gacgatgcac tcaaggcgag tccatgttta agtatcccgc agtcagaatg gaatcataca
    31141 ctgactcgtc agtacgttat cgataatgag aaattagaaa acttctctgt tgatactcag
    31201 ttgcaaagta aattcgcaac cggctcggtt gaacacactc tgctgaccgg cgttgacttt
    31261 atgcgtatgc gtaatgatat tgactcctgg ttcggttacg ccggttccgt tgcaccgtct
    31321 gatatctata atttagaccg tagtgacttt gattttggtg ctcacccgga tccgtctggc
    31381 ccataccgcg ttttgcttaa acagaaacaa accggtctgt atgttcagga tcaggcgcag
    31441 tgggataagg tgctggtgac tctgggtggt cgctatgact gggcggaaca gtcatctttc
    31501 aaccgtgatt acggtaataa atccgatcgt gatgacaaac agttcacctg gcgtggtggc
    31561 gtaaactacc tgttcgacaa cggggtaacg ccttacttca gctacagtga gtcgtttgag
    31621 ccagcatctt tgacagatgc aaacggtgat ctgtttgcac cttcgaaagg caaacagtat
    31681 gaagttggtg tgaaatatgt gccggaagat cgcccaattg tgctgacggg cgcactgtat
    31741 cagcttacca aaaccaacaa cctgatggcg gatccgaata atcccaattt ctcgattgaa
    31801 ggcggtgaga ttcgcgctcg tggtgtagaa ctggaagcaa aagcggcgct gtcggcgagt
    31861 gttaacgtag taggttctta tacttacacc gatgcggaat acaccaccga cactacctat
    31921 aaaggcaata cgcctgcaca ggtgccaaaa cacatggctt cgctgtgggc tgactatacc
    31981 ttctttgacg gtccgctttc aggtctgacg ctgggcaccg gtggtcgtta tactggctcc
    32041 agctatggtg atccggctaa ctcctttaaa gtgggaagtt atacggtcgt ggatgcgtta
    32101 gtgcgttatg atctggcgcg agtcggcatg gctggctcca acgtggcgct gcatgtcaac
    32161 aacctgttcg atcgtgaata cgtcgccagc tgctttaaca cctatggctg cttctggggc
    32221 gcagaacgtc aggtcgttgc aaccgcaacc ttccgtttct aatttctctt ttggggcacg
    32281 gatttccgtg cccgtttcac aagttggctg ttatgcagga atacacgaat cattccgata
    32341 ccacttttgc actgcgtaat atctcctttc gtgtgcccgg gcgcacgctt ttgcatccgc
    32401 tgtcgttaac ctttcctgcc gggaaagtga ccggtctgat tggtcacaac ggttctggta
    32461 aatccactct gctcaaaatg cttggtcgtc accagccacc gtcggaaggg gagattctcc
    32521 ttgatgccca gccgctggaa agctggagca gcaaagcgtt tgcccgcaaa gtggcttatt
    32581 tgccgcagca gcttcctccg gcagaaggga tgaccgtgcg tgaactggtg gcgattggtc
    32641 gttacccgtg gcatggcgca ctggggcgct ttggtgcggc agatcgcgaa aaggtcgaag
    32701 aggcgatttc gctggttggc ttaaaaccgc tggcgcatcg gctggtcgat agtctctctg
    32761 gcggcgaacg tcagcgggcg tggatcgcta tgctggtggc acaggatagc cgttgtctgt
    32821 tgctcgacga accgacctcg gcgctggata tcgcccacca ggttgatgtg ctggcgctgg
    32881 tgcaccgttt aagtcaggag cgtggcctga cggtcattgc cgtgttgcac gatatcaata
    32941 tggcggcacg ctactgtgat tatctggtcg ccctgcgcgg tggtgaaatg attgctcagg
    33001 gaacgcctgc ggaaattatg cgcggcgaaa cccttgaaat gatttatggc atcccgatgg
    33061 gtattttgcc gcatccggcg ggtgctgcac ctgtgagttt tgtttattga tgagcggctt
    33121 acctcttatt tcgcgccgtc gactgttaac ggcgatggcg ctttctccgt tgttatggca
    33181 gatgaatacc gcccacgcgg cggctattga tcccaatcgt attgtggcgc tggagtggtt
    33241 gccggtggaa ttactgctgg cgctcggcat cgtgccttac ggcgtggcgg ataccatcaa
    33301 ctatcgcctg tgggtcagcg aaccaccatt gccggactca gtgatcgacg tcggtttgcg
    33361 cacagaacct aaccttgaac tgctgaccga aatgaaacca tcgtttatgg tctggtcggc
    33421 aggatatggc ccttcaccag aaatgctggc acgcattgcg ccggggcgcg gatttaactt
    33481 cagcgatggc aaacagccgc tggcgatggc gcgtaaatca ctgacggaaa tggcagattt
    33541 acttaacctg caaagcgcag cagaaacgca tttagcgcac tatgaagact ttatccgcag
    33601 catgaaaccc cgctttgtga agcgtggtgc acgcccgtta ttgctgacga cacttatcga
    33661 tccgcgccat atgttggtct tcggtccaaa cagcctgttc caggaaattc ttgatgagta
    33721 cggcatccca aatgcctggc aaggggaaac caacttctgg ggcagtaccg ctgtcagtat
    33781 cgatcgtctg gcagcgtata aagacgttga tgtgctctgt tttgatcacg acaacagcaa
    33841 agacatggac accctaatgg caaccccgct gtggcaggcc atgccgtttg tccgcgccgg
    33901 acgctttcag cgcgttccgg cggtctggtt ctatggtgcg acgctttctg caatgcactt
    33961 tgtgcgtatt ctggataacg ccatcggagg taaagcgtga gtaaacgaat tgcgcttttt
    34021 ccggtgttat tgctggtgct gctggtggtt gctgctgcgg cgttgacctg gatgaacttc
    34081 tcgcaggcgc tgccgcgcag ccagtgggcg caggccgcct ggtcgccgga tattgacgtc
    34141 atcgagcaga tgatttttca ctacagcttg ttgccgcgtc tggcgatttc gctgctggtg
    34201 ggcgcgggcc tggggctggt gggcgtgctg tttcagcaag tgctgcgtaa cccgctggcg
    34261 gaaccgacga cgttgggcgt tgcaacaggc gcgcaactgg ggattaccgt caccacgctc
    34321 tgggcgatcc caggtgctat ggcgagccag tttgctgcat tggcaggggc ttgtgtggtt
    34381 ggcttaatcg tctttggcgt cgcgtggggg aaacggcttt cgccggtaac gctgatcctc
    34441 gcggggctgg tagtgagcct ttattgcggc gcaatcaatc agttactggt tatcttccat
    34501 catgaccaac tgcaaagcat gttcctgtgg agcaccggaa cgctgacgca aaccgactgg
    34561 ggcggcgttg agcgtttatg gccgcagctg ctgggcggcg tgatgctgac gttattgcta
    34621 cttcgcccgt taactctgat ggggcttgat gatggagtgg cgcgcaatct cgggctggcc
    34681 ttgtcgctgg cgcgtctggc ggcgttgtcg ctggcgattg tcatcagtgc gctgctggtg
    34741 aacgcggtgg ggattatcgg ctttatcggt ttgttcgcgc cactgctggc gaaaatgctg
    34801 ggggcgcggc gtctgctacc aaggttgatg ctggcgtcgc tgattggtgc gctgatcctc
    34861 tggctttccg atcaaatcat cctctggctg actcgcgtgt ggatggaagt gtccaccggt
    34921 tcggtcactg cgttgatcgg tgcgccgctg ctactgtggt tgctgccgcg tttacgcagc
    34981 attagcgcgc cggatatgaa ggtcaacgat cgtgtcgaga ctgaacgcca acacgtgctg
    35041 gcgtttgccc tcgcgggcgg cgtgctgctg ttgatggctg tggtggtggc gctgtcgttt
    35101 ggtcgtgatg cgcacggctg gacgtgggcg agcggggcgc tgctcgatga tttaatgccc
    35161 tggcgctggc cgcgaattat ggcggcactg tttgcgggca tcatgctggc agtggcgggc
    35221 tgtattattc agcggctgac cggaaacccg atggcaagcc cggaagtgct ggggattagt
    35281 tccggcgcgg cgtttggtgt ggtgttgatg ctgtttttgg tgccgggtaa tgcctttggc
    35341 tggctgttac ccgcgggcag cctcggggcg gcggtgacgc tgttgatcat tatgatcgcc
    35401 gccggacgcg gtggattttc cccacaccgc atgttactgg cggggatggc gttaagtacc
    35461 gcgttcacca tgcttttgat gatgttgcag gcaagtggtg acccgcgaat ggcgcaagtt
    35521 ctgacctgga tttccggttc gacctacaac gcgaccgagg cgcaggtctg gcgcacggga
    35581 attgtgatgg taattttgct ggcgattacc ccgctgtgcc gccgctggct gaccatttta
    35641 ccgctgggcg gtgataccgc acgagccgta ggaatggcgc tgacgccgac gcgaattgcg
    35701 ctgctgctgt tagcggcttg cctgacagcg actgcgacaa tgaccattgg accgttgagt
    35761 tttgttggtt taatggcacc gcatattgcg cggatgatgg gctttcgacg gacgatgcca
    35821 cacatcgtaa tttcggcgct ggtgggtggt ttactgctgg tgttcgctga ctggtgtggg
    35881 cggatggtat tgtttccatt ccagatcccg gcggggctgc tgtcgacctt tatcggcgcg
    35941 ccatatttta tctatttgtt gagaaagcag agccgttaat agaatgcctc gcccggtggg
    36001 cgaggcaaaa tcattacaac ttcgcaaaca cccgacgtgc agcatcgatg gtgttattga
    36061 tatcttccat gctatgcgcc acggacataa agcccgcttc aaacgctgac ggtgccaggt
    36121 aaacaccttc gtccagcatc atatggaaga aacgcttaaa gcgttccacg tcacaggcca
    36181 tcacatcctg atagcacgtc acggactcgg cgtcggtaaa gaaaataccg aacatgccgc
    36241 caacgtggtt taccaccagc ggaattccgg cttcttctgc cgcttccagc agaccttctg
    36301 ccagacgtgt tgtcagctca tccagcgttt cgtgaacgcc cggctgtgcg acttcattca
    36361 gacaggcgaa accggctgcc atcgcaatcg ggttaccgga aagcgtaccc gcctgatata
    36421 ccggaccagt cggggccagc gcatccatta catcacgacg accaccgaat gcgcctaccg
    36481 gcattccacc gccgatgatt ttgccgaggc aggttaaatc tggcactacg ccgtaataat
    36541 cctgtgcgcc agctagcgct acgcggaaac cggtcatcac ttcatcgatg atcagcaacg
    36601 cgccaaattc gtcgcacagc gcgcgcagac ctggcaggaa ctctggcagc ggtggaacgc
    36661 agttcatatt gcctgccacc ggctcgacga taatacaggc aatctcttgc gggtattgct
    36721 caaacgcggc gcgtacagag gccagatcgt tataagtaca ggttaaggta tgtttggcga
    36781 aatctgccgg aacgcccggc gaatttggct ggcctaacgt gagtgcgcca gaaccggctt
    36841 tcaccagcag gcagtcagcg tgaccgtggt aacaaccttc aaatttaata attttgtcgc
    36901 gaccggtaaa accacgggcc aggcggatgg cgctcatcgt cgcttcagtg ccggagttca
    36961 ccatgcgcac catatccatg gtcgggacca gttcggtcac cagttgcgcc attttcactt
    37021 ccatttcggt tggtgcacca aagcttaaac cacgctcggc ggcttcaatc acggcattgc
    37081 ggatcgccgg atggttatgg cccagcacca tcggccccca ggaaccgaca taatcgatat
    37141 aggctttgcc atcaacatcg tacagataag cgccgtccgc tttttcgata aacagtggag
    37201 tgccgcccac gccagtaaag gcgcgaacag gggagttcac accgccaggg atcagctcgc
    37261 gcgctgcgct gtaaagattt tcagacttac tcatggaggg ttcctgattc gtagaaaaag
    37321 tgaatggctg ctattctatg ttattcataa caagttaaat actcgtcaaa catcaggctg
    37381 cttgtactgg tgcaatcctg atttcttaga gtataaaagt tttgtgcatt tgaaacatta
    37441 cgctttgcaa aggattttca tggaacgtgc gagtaaaatg ccgtcatctt atttgtatga
    37501 ccaataagtg atcattggat gaaaactgat actccctctt tagaaacacc gcaggccgcg
    37561 cgcctgcgac gcagacaact gattcgccaa cttcttgagc gcgataaaac cccgttagcc
    37621 attttgttta tggcggcagt cgtcggcacg cttgtcgggc tggctgcggt tgcttttgac
    37681 aaaggtgtcg cctggttgca gaaccaacgt atgggggcac tggtacatac tgctgataat
    37741 tatccgcttc tgttaaccgt cgcttttctc tgttcggcgg tgctggcgat gttcggctac
    37801 tttttggtgc gcaaatacgc gccggaagca ggtggttcgg ggatcccaga aattgaaggg
    37861 gcgctggaag atcaacgtcc cgttcgctgg tggcgtgtgt tgccggtgaa attctttggc
    37921 gggctgggaa cacttggcgg aggcatggtg ttggggcgcg aagggccaac cgtgcagata
    37981 ggcggcaata tcgggcggat ggtactcgat gttttccgcc tgaaaggtga cgaagctcgt
    38041 catacgctac tggcaaccgg tgcggcggcg ggtctggcgg cggcctttaa cgccccgcta
    38101 gcgggtattt tgtttattat cgaagagatg cgtccgcagt ttcgctatac gttaatttcg
    38161 attaaagcgg tatttattgg cgtcattatg tcgaccatta tgtaccggat ttttaatcat
    38221 gaagttgcat tgattgacgt cggtaaactt tctgacgcgc cgcttaatac gctgtggctt
    38281 tatctgatcc tcggtattat ttttggcatt ttcggcccta tttttaataa atgggtgctg
    38341 gggatgcagg atttgctgca ccgtgtgcac ggcggcaata ttaccaaatg ggtactgatg
    38401 ggcggtgcaa ttggcggtct gtgtggattg ctggggtttg tggcaccagc aacgtcgggc
    38461 ggcggcttta acctgattcc tatcgctacc gcagggaatt tcagcatggg aatgctggtg
    38521 tttattttcg tcgcgcgggt cattaccacc ttactctgtt tctcttccgg cgcgccgggc
    38581 ggtatttttg cccctatgct ggcgctgggt actgtgctgg gaaccgcttt cggaatggtt
    38641 gccgttgagc tgtttccgca atatcacctt gaagcaggca cttttgctat tgccggaatg
    38701 ggggcgttac tggcggcatc tattcgtgcg ccgttaacgg ggattattct ggttctggaa
    38761 atgaccgata actaccagct cattttgcca atgattatta ccggtcttgg cgcaacacta
    38821 ttagcgcaat ttaccggcgg gaaaccgcta tactcggcga ttcttgcgcg cacgctggcg
    38881 aaacaggaag ctgagcaact ggcgcgaagc aaggccgcat cagccagcga gaatacttga
    38941 acgaaatacc agggtattag ataatggcga ttattattgg gttagaattt gcccaattgc
    39001 cgatgtcgtt tggagcaaaa tatgagtgat gacgtagcac tgccgctgga gtttaccgac
    39061 gcagcagcca acaaagttaa aagcctgatc gctgacgaag ataacccgaa tctgaaatta
    39121 cgcgtgtata tcaccggtgg cggttgcagc ggcttccagt atggtttcac ctttgatgat
    39181 caggtgaacg aaggcgatat gaccatcgaa aaacagggcg ttggcctggt ggttgatccg
    39241 atgagcctgc aatatctggt cggcggttcc gttgactata ccgaaggttt ggaaggttct
    39301 cgtttcatcg tgaccaaccc gaacgcgaaa agtacctgcg gttgcggttc ttcctttagt
    39361 atctaatctg ctgttcatgt gccggataaa ctatccggca catcttctct cttcaacgac
    39421 cattctcatc cagcgcaaac gtcggtagct taagatgcca acgaatagcc gccagccgaa
    39481 tcaatagcgt cacgaccatg cccatcatac tggctgtttc cagtggtacg gaaaatgtgt
    39541 aataagccgt agcgtggaca ataccgccga taatacaggc agttgcgtag atttctgtac
    39601 gtaaaatcat ggggatttcg cgggccagta catcacgaat gatcccgccg ccaacgccag
    39661 taatgacgcc catacaaacc gcgattaacg gaccggcttc cgcattaaag gctttattca
    39721 cgccaatgcc gacaaacacc gccagaccaa cggcgtccag caccggcaac atccattttg
    39781 gtaagcgtct tggctggcgc accagcacga tggtcagcat gctggtgacc attgcaacga
    39841 ccagatcggt gggatctttc acccaaaata ccgggccgtg atccagcgcc atgtcgcgaa
    39901 ttgttccgcc gcctactgcg gttaccacgc ccagtaccag aacaccaaaa gggtccatac
    39961 gcaattttcc ggctaacaaa acgccggaga tggcaaatac cgctgtgccg actatatcca
    40021 gccaatagac gagcattgtt caatccccac tgagcacctg aaaaggtcag gcgctaatct
    40081 acctgtgaaa gcgcattaca gagctgttgt gcagcgagga taatacgtgg gcttgcacgt
    40141 tcaaaccagt cactcgtgag aggaataacg ggaattttga gctgttcacc ccagtattgt
    40201 ttgattttag gaatttggtc cggtccgcct gtaatgacaa tcgcctgtgg cgagcgtgct
    40261 aacacctgtt cacggctaac ttgcggccag ggaacccggc tgtctttaaa gatgttttct
    40321 ccgccacaaa cttcgagtac ctggttttga atcgactctt ttccactggt aaatggcgga
    40381 ttaatgccga attgcagaaa aacacgtttt ttaggtttat cagcatattg cgctttcaat
    40441 tgcgcgtact gatccagcag ggattgcgcg gcttgttcgg ccttatccgg ttgcggactc
    40501 cagggggcca gttgacgtaa cgcattggca atttgttcaa tgcttgtcgc atcgacccac
    40561 atcactttta ttcccagcga agccagctgg tcaacctgcc gctcggcatt acctccacgc
    40621 caggcaatca ccagatcggg tttcagcgcg acaatgcgtt ccagattcat cccctgccag
    40681 gtggaaacct gctcaatctt ttgcgcttgt ggaggatagt cggaatagct gctgacccca
    40741 accggcgtga tcccggcggc aaaggcaagt tcagtgttgg cgggagaaag cgtgatgacg
    40801 cgcggcgcgg cattgagcca cagcggcgcg agaaaagaca gggcgaccag cgccctgaac
    40861 agtgacttag ccatgtgcca gtttctgcac cagtgactca accatcaggc tggactgttt
    40921 agcggcaaca gccaggaact catcgaagct aagatgagac tgttgatcgg ccacgtcgga
    40981 gatggcgcgt actacgacaa acgggacgtt gaaattgtgg cagacatggg cgattgccgt
    41041 cgcttccatc tctacagcaa tggcctgtgg gaagttgtgg cggattttcg ccagaccaac
    41101 agaaccgttg atgaatgcgt cgccgctaac aatcaggcca cgtacagcgt taagattcag
    41161 ttcggcaatg caggcctcag cggcagcgat cagtttatcg tcagctttaa agcctgccgg
    41221 gcagcctggt aactggccgt attcataacc aaatgccgtg acatccgcgt cgtgataacg
    41281 cgcttcgtcc gagacaacga tatcgcccac tttcagcgtt ggtgccaggc caccggcaga
    41341 accggtgtta ataatcacat ctggcttgca gtgttccaac agcagagtgg cacccagcgc
    41401 cgcagcgact ttaccgatgc ccgatttcag aagcgcaacc tcggttccat tcagttggcc
    41461 ggtatagatt tcgcaaccgc ccagactgat agtttgacgg ttttcgattt tgtcacgcag
    41521 cagcgtaact tcttcttcca ttgcaccaat gatgccgatt ttcatagatt tactcgcgat
    41581 aagcccgatt tgaaggcata gtttaccatg cgcttacggg gaagcgtatt tctcacgcgg
    41641 gagaggacat ggcacagatt gatttccgaa aaaaaataaa ctggcatcgt cgttaccgtt
    41701 caccacaggg cgttaaaacc gaacatgaga tcctgcggat cttcgagagc gatcgcgggc
    41761 gtatcatcaa ctctccggca attcgtcgtc tgcaacaaaa gacccaggtt tttccactgg
    41821 agcgcaatgc cgccgtgcgc acgcgtctta cccactcgat ggaagtccag caggtggggc
    41881 gctacatcgc caaagaaatt ttaagccgtc tgaaagagct taaattgctg gaagcatacg
    41941 gcctggatga actgaccggc ccctttgaaa gcattgttga gatgtcatgc cttatgcacg
    42001 atatcggcaa tccgccgttt ggtcattttg gcgaagcggc gataaatgac tggtttcgcc
    42061 agcgtttgca cccggaagat gccgaaagcc agcctctgac tgacgatcgc
//
LOCUS       DAMZFT010000026        41896 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.026, whole genome shotgun sequence.
ACCESSION   DAMZFT010000026 DAMZFT010000000
VERSION     DAMZFT010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 41896)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41896)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41896
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.026"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(154..849)
                     /gene="aqpZ"
                     /locus_tag="PN977_001541"
     CDS             complement(154..849)
                     /gene="aqpZ"
                     /locus_tag="PN977_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin Z"
                     /protein_id="HDI6041588.1"
                     /translation="MFRKLAAECFGTFWLVFGGCGSAVLAAGFPELGIGFAGVALAFG
                     LTVLTMAFAVGHISGGHFNPAVTIGLWAGGRFPAKEVVGYVIAQVVGGIVAAALLYLI
                     ASGKTGFDAAASGFASNGYGEHSPGGYSMLSALVVELVLSAGFLLVIHGATDKFAPAG
                     FAPIAIGLALTLIHLISIPVTNTSVNPARSTAVAIFQGGWALEQLWFFWVVPIVGGII
                     GGLIYRTLLEKRN"
     gene            1275..2933
                     /locus_tag="PN977_001542"
     CDS             1275..2933
                     /locus_tag="PN977_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent endonuclease"
                     /protein_id="HDI6041589.1"
                     /translation="MILERVEIVGFRGINRLSLMLEQNNVLIGENAWGKSSLLDALTL
                     LLSPESDLYHFERDDFWFPPGDINGREHHLHIILTFRESLPGRHRVRRYRPLEACWTP
                     CTDGYHRIFYRLEGESAEDGSVMTLRSFLDKDGHPIDVEDINDQARHLVRLMPVLRLR
                     DARFMRRIRNGTVPNVPNVEVTARQLDFLARELSSHPQNLSDGQIRQGLSAMVQLLEH
                     YFSEQGAGQARYRLMRRRASNEQRSWRYLDIINRMIDRPGGRSYRVILLGLFATLLQA
                     KGTLRLDKDARPLLLIEDPETRLHPIMLSVAWHLLNLLPLQRIATTNSGELLSLTPVE
                     HVCRLVRESSRVAAWRLGPSGLSTEDSRRISFHIRFNRPSSLFARCWLLVEGETETWV
                     INELARQCGHHFDAEGIKVIEFAQSGLKPLVKFARRMGIEWHVLVDGDEAGKKYAATV
                     RSLLNNDREAEREHLTALPALDMEHFMYRQGFSDVFHRVAQIPENVPMNLRKIISKAI
                     HRSSKPDLAIEVAMEAGRRGVDSVPTLLKKMFSRVLWLARGRAD"
     gene            complement(2930..3886)
                     /locus_tag="PN977_001543"
     CDS             complement(2930..3886)
                     /locus_tag="PN977_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001322270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirK/YbjX family protein"
                     /protein_id="HDI6041590.1"
                     /translation="MNMSQLTERTFTPSESLSSLSLFLSLARGQCRPGKFWHRRSFRQ
                     KFLLRSLIMPRLSVEWMNELSHWPNLNVLLTRQPRLPVRLHRPYLAANLSRKQLLEAL
                     RYHYALLRGCMSAEEFSLYLNTPGLQLAKLEGKNGEQFTLELTMMISMDKEGDSTILF
                     RNSEGIPLAEITFTLCEYQGKRTMFIGGLQGAKWEIPHQEIQNATKACHGLFPKRLVM
                     EAACLFAQRLQVEQIIAVSNETHIYRSLRYRDKEGKIHADYNAFWESVGGVCDAERHY
                     RLPAQIARKEIAEIASKKRAEYRRRYEMLDAIQPQMATMFRG"
     gene            4037..5152
                     /gene="macA"
                     /locus_tag="PN977_001544"
     CDS             4037..5152
                     /gene="macA"
                     /locus_tag="PN977_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide transporter subunit MacA"
                     /protein_id="HDI6041591.1"
                     /translation="MKKRKTVKKRYVIALVIVIAGLITLWRILNAPVPTYQTLIVRPG
                     DLQQSVLATGKLDALRKVDVGAQVSGQLKTLSVAIGDKVKKDQLLGVIDPEQAENQIK
                     EVEATLMELRAQRQQAEAELKLARVTYSRQQRLAQTQAVSQQDLDTAATEMAVKQAQI
                     GTIDAQIKRNQASLDTAKTNLDYTRIVAPMAGEVTQITTLQGQTVIAAQQAPNILTLA
                     DMSTMLVKAQVSEADVIHLKPGQKAWFTVLGDPLTRYEGQIKDVLPTPEKVNDAIFYY
                     ARFEVPNPNGLLRLDMTAQVHIQLTDVKNVLTIPLLALGDPVGDNRYKVKLLRNGETR
                     EREVTIGARNDTDVEIVKGLEAGDEVVIGEAKPGAAQ"
     gene            5149..7095
                     /gene="macB"
                     /locus_tag="PN977_001545"
     CDS             5149..7095
                     /gene="macB"
                     /locus_tag="PN977_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide ABC transporter ATP-binding
                     protein/permease MacB"
                     /protein_id="HDI6041592.1"
                     /translation="MTPLLELKDIRRSYPAGDEQVEVLKGISLDIYAGEMVAIVGASG
                     SGKSTLMNILGCLDKATSGTYRVAGQDVATLDADALAQLRREHFGFIFQRYHLLSHLT
                     AEQNVEVPAVYAGLERKQRLLRAQELLQRLGLEDRTEYYPAQLSGGQQQRVSIARALM
                     NGGQVILADEPTGALDSHSGEEVMAILHQLRDRGHTVIIVTHDPQVAAQAERVIEIRD
                     GEIVHNPPAVEKVNATGGTEPVVNTASGWRQFVSGFNEALTMAWRALAANKMRTLLTM
                     LGIIIGIASVVSIVVVGDAAKQMVLADIRSIGTNTIDVYPGKDYGDDDPQYQQALKYD
                     DLIAIQKQPWVASATPAVSQNLRLRYNNVDVAASANGVSGDYFNVYGMTFSEGNTFNQ
                     EQLNGRAQVVVLDSNTRRQLFPHKADVVGEVILVGNMPARVIGVAEEKQSMFGSSKVL
                     RVWLPYSTMSGRVMGQSWLNSITVRVKEGFDSAEAEQQLTRLLSLRHGKKDFFTWNMD
                     GVLKTVEKTTRTLQLFLTLVAVISLVVGGIGVMNIMLVSVTERTREIGIRMAVGARAS
                     DVLQQFLIEAVLVCLVGGALGITLSLLIAFTLQLFLPGWEIGFSPLALLLAFLCSTVT
                     GILFGWLPARNAARLDPVDALARE"
     gene            complement(7168..7392)
                     /gene="cspD"
                     /locus_tag="PN977_001546"
     CDS             complement(7168..7392)
                     /gene="cspD"
                     /locus_tag="PN977_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspD"
                     /protein_id="HDI6041593.1"
                     /translation="MEKGTVKWFNNAKGFGFICPEGGGEDIFAHYSTIQMDGYRTLKA
                     GQSVQFDVHQGPKGNHASVIVPVEVEAAVA"
     gene            7715..8035
                     /gene="clpS"
                     /locus_tag="PN977_001547"
     CDS             7715..8035
                     /gene="clpS"
                     /locus_tag="PN977_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HDI6041594.1"
                     /translation="MGKTNDWLDFDQLAEEKVRDALKPPSMYKVILVNDDYTPMEFVI
                     DVLQKFFSYDVERATQLMLAVHYQGKAICGVFTAEVAETKVAMVNKYARENEHPLLCT
                     LEKA"
     gene            8066..10342
                     /gene="clpA"
                     /locus_tag="PN977_001548"
     CDS             8066..10342
                     /gene="clpA"
                     /locus_tag="PN977_001548"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HDI6041595.1"
                     /translation="MLNQELELSLNMAFARAREHRHEFMTVEHLLLALLSNPSAREAL
                     EACSVDLVALRQELEAFIEQTTPVLPASEEERDTQPTLSFQRVLQRAVFHVQSSGRNE
                     VTGANVLVAIFSEQESQAAYLLRKHEVSRLDVVNFISHGTRKDEPTQSSDPGSQPNSE
                     EQAGGEERMENFTTNLNQLARVGGIDPLIGREKELERAIQVLCRRRKNNPLLVGESGV
                     GKTAIAEGLAWRIVQGDVPEVMADCTIYSLDIGSLLAGTKYRGDFEKRFKALLKQLEQ
                     DTNSILFIDEIHTIIGAGAASGGQVDAANLIKPLLSSGKIRVIGSTTYQEFSNIFEKD
                     RALARRFQKIDITEPSIEETVQIINGLKPKYEAHHDVRYTAKAVRAAVELAVKYINDR
                     HLPDKAIDVIDEAGARARLMPVSKRKKTVNVADIESVVARIARIPEKSVSQSDRDTLK
                     NLGDRLKMLVFGQDKAIEALTEAIKMARAGLGHEHKPVGSFLFAGPTGVGKTEVTVQL
                     SKALGIELLRFDMSEYMERHTVSRLIGAPPGYVGFDQGGLLTDAVIKHPHAVLLLDEI
                     EKAHPDVFNILLQVMDNGTLTDNNGRKADFRNVVLVMTTNAGVRETERKSIGLIHQDN
                     STDAMEEIKKIFTPEFRNRLDNIIWFDHLSTDVIHQVVDKFIVELQVQLDQKGVSLEV
                     SQEARNWLAEKGYDRAMGARPMARVIQDNLKKPLANELLFGSLVDGGQVTVALDKEKN
                     ELTYGFQSAQKHKAEAAH"
     gene            complement(10626..10713)
                     /locus_tag="PN977_001549"
     tRNA            complement(10626..10713)
                     /locus_tag="PN977_001549"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10677..10679),aa:Ser,seq:gga)
     gene            complement(10967..11185)
                     /gene="infA"
                     /locus_tag="PN977_001550"
     CDS             complement(10967..11185)
                     /gene="infA"
                     /locus_tag="PN977_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HDI6041596.1"
                     /translation="MAKEDNIEMQGTVLETLPNTMFRVELENGHVVTAHISGKMRKNY
                     IRILTGDKVTVELTPYDLSKGRIVFRSR"
     gene            complement(11470..12174)
                     /gene="aat"
                     /locus_tag="PN977_001551"
     CDS             complement(11470..12174)
                     /gene="aat"
                     /locus_tag="PN977_001551"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HDI6041597.1"
                     /translation="MRLVQLSRHSIAFPSPEGALREPNGLLALGGDLSPARLLMAYQR
                     GIFPWFSPGDPILWWSPDPRAVLWPESLHISRSMKRFHKRSPYRVTMNYAFGQVIEGC
                     ASDREEGTWITRGVVEAYHRLHELGHAHSIEVWREDELVGGMYGVAQGTLFCGESMFS
                     RMENASKTALLVFCEEFIGHGGKLIDCQVLNDHTASLGACEIPRRDYLNYLNQMRLGR
                     LPNNFWVPRCLFSPQE"
     gene            complement(12216..13937)
                     /gene="cydC"
                     /locus_tag="PN977_001552"
     CDS             complement(12216..13937)
                     /gene="cydC"
                     /locus_tag="PN977_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter ATP-binding
                     protein/permease CydC"
                     /protein_id="HDI6041598.1"
                     /translation="MRALLPYLALYKRHKWMLSLGIVLAIVTLLASIGLLTLSGWFLS
                     ASAVAGVAGLYSFNYMLPAAGVRGAAITRTAGRYFERLVSHDATFRVLQHLRIYTFSK
                     LLPLSPAGLARYRQGELLNRVVADVDTLDHLYLRVISPLVGAFVVIMVVTIGLSFLDF
                     TLAFTLGGIMLLTLFLMPPLFYRAGKSTGQNLTHLRGQYRQQLTAWLQGQAELTIFGA
                     SDRYRTQLENTEIQWLESQRRQSELTALSQAIMLLIGALAVILMLWMASGGVGGNAQP
                     GALIALFVFCALAAFEALAPVTGAFQHLGQVIASAVRITDLTDQKPEVTFPDTQTRVA
                     DRVSLTLRDVQFTYPEQSQQALKGISLQVNAGEHIAILGRTGCGKSTLLQLLTRAWDP
                     QQGEILLNDSPIASLNEATLRQTISVVPQRVHLFSATLRDNLLLASPDSSDEALAEIL
                     RRVGLEKLLEDAGLNSWLGEGGRQLSGGELRRLAIARALLHDAPLVLLDEPTEGLDAT
                     TESQILELLAEMMREKTVLMVTHRLRGLSRFQQIIVMDNGQIIEQGTHAELLARQGRY
                     YQFKQGL"
     gene            complement(13938..15704)
                     /gene="cydD"
                     /locus_tag="PN977_001553"
     CDS             complement(13938..15704)
                     /gene="cydD"
                     /locus_tag="PN977_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter
                     permease/ATP-binding protein CydD"
                     /protein_id="HDI6041599.1"
                     /translation="MNKSRQKELTRWLKQQSVISQRWLNISRLLGFVSGILIIAQAWF
                     MARILQHMIMENIPREALLLPFTLLVLTFVLRAWVVWLRERVGYHAGQHIRFAIRRQV
                     LDRLQQAGPAWIQGKPAGSWATLVLEQIDDMHDYYARYLPQMALAVSVPLLIVVAIFP
                     SNWAAALILLGTAPLIPLFMALVGMGAADANRRNFLALARLSGHFLDRLRGMETLRIF
                     GRGEAEIESIRSASEDFRQRTMEVLRLAFLSSGILEFFTSLSIALVAVYFGFSYLGEL
                     DFGHYDTGVTLAAGFLALILAPEFFQPLRDLGTFYHAKAQAVGAADSLKTFMETPLAH
                     PQRGEAELASTDPVTIEAEELFITSPEGKTLAGPLNFTLPAGQRAVLVGRSGSGKSSL
                     LNALSGFLSYQGSLRINGIELRDLSPESWRKHLSWVGQNPQLPAATLRDNVLLARPDA
                     NEHELQAALDNAWVSEFLPLLPQGVDTPVGDQAARLSVGQAQRVAVARALLNPCSLLL
                     LDEPAASLDAHSEQRVMEALNAASLRQTTLMVTHQLEDLADWDVIWVMQDGQIIEQGR
                     YAELSVAGGPFATLLAHRQEEI"
     gene            complement(15827..16792)
                     /gene="trxB"
                     /locus_tag="PN977_001554"
     CDS             complement(15827..16792)
                     /gene="trxB"
                     /locus_tag="PN977_001554"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HDI6041600.1"
                     /translation="MGTTKHSKLLILGSGPAGYTAAVYAARANLQPVLITGMEKGGQL
                     TTTTEVENWPGDPNDLTGPLLMERMHEHATKFETEIIFDHINKVDLQNRPFRLTGDSG
                     EYTCDALIIATGASARYLGLPSEEAFKGRGVSACATCDGFFYRNQKVAVIGGGNTAVE
                     EALYLSNIASEVHLIHRRDGFRAEKILIKRLMDKVENGNIILHTNRTLEEVTGDQMGV
                     TGVRLRDTQNSDNIESLDVAGLFVAIGHSPNTAIFEGQLELENGYIKVQSGIHGNATQ
                     TSIPGVFAAGDVMDHIYRQAITSAGTGCMAALDAERYLDGLADAK"
     gene            17337..17831
                     /gene="lrp"
                     /locus_tag="PN977_001555"
     CDS             17337..17831
                     /gene="lrp"
                     /locus_tag="PN977_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001591745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-responsive transcriptional regulator
                     Lrp"
                     /protein_id="HDI6041601.1"
                     /translation="MVDSKKRPGKDLDRIDRNILNELQKDGRISNVELSKRVGLSPTP
                     CLERVRRLERQGFIQGYTALLNPHYLDASLLVFVEITLNRGAPDVFEQFNTAVQKLEE
                     IQECHLVSGDFDYLLKTRVPDMSAYRKLLGETLLRLPGVNDTRTYVVMEEVKQSNRLV
                     IKTR"
     gene            17966..22075
                     /gene="ftsK"
                     /locus_tag="PN977_001556"
     CDS             17966..22075
                     /gene="ftsK"
                     /locus_tag="PN977_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HDI6041602.1"
                     /translation="MSQEYTEDKEVTLTKLSSGRRLLEALLILIVLFAVWLMAALLSF
                     NPSDPSWSQTAWHEPIHNLGGMPGAWLADTLFFIFGVMAYTIPVIIVGGCWFAWRHQS
                     SDEYIDYFAVSLRIIGVLALILTSCGLAAINADDIWYFASGGVIGSLLSTTLQPLLHS
                     SGGTIALLCVWAAGLTLFTGWSWVTIAEKLGGWILNILTFASNRTRRDDTWVDEDEYE
                     DDEEYEEDESHGKQHESRRARILRGALARRKRLAEKFINPMGRQTDAALFSGKRMDDD
                     EEITYTARGVAADPDDVLFSGNRATQPEYDEYDPLLNGAPITEPVAVAAAATTATQSW
                     AAPVEPVTQTPPVASVDVPPAQPTVAWQPVPGPQTGEPVIAPAPEGYPQQPQYAQPAV
                     QYNEPLQQPVQPQQPYYAPAAEQSAQQPYYAPAPEQSAQQPYYAPAPEQSVAGNAWQA
                     EEQQSTFAPQSTYQTEQTYQQPAAQEPLYQQPQPVEQQPVVEPEPVVEETKPARPPLY
                     YFEEVEEKRAREREQLAAWYQPIPEPVKEPEPIKSSLKAPSVAAVPPVEAAAAVSPLA
                     SGVKKATLATGAAATVAAPVFSLANSGGPRPQVKEGIGPQLPRPKRIRVPTRRELASY
                     GIKLPSQRAAEEKAREAQRNQYDSGDQYNDDEIDAMQQDELARQFAQTQQQRYGEQYQ
                     HDVPVNAEDADAAAEAELARQFAQTQQQRYSGEQPAGANPFTLDDFEFSPMKALLDDG
                     PHEPLFTPIVEPVQQPQQPIAPQQQYQQPQQPVAPQPQYQQPQQPVAPQQQYQQPQQP
                     VAPQPQYQQPQQPVAPQPQYQQPQQPVAPQQQYQQPQQPVAPQPQYQQPQQPVAPQPQ
                     DTLLHPLLMRNGDSRPLHKPTTPLPSLDLLTPPPSEVEPVDTFALEQMARLVEARLAD
                     FRIKADVVNYSPGPVITRFELNLAPGVKAARISNLSRDLARSLSTVAVRVVEVIPGKP
                     YVGLELPNKKRQTVYLREVLDNAKFRDNPSPLTVVLGKDIAGEPVVADLAKMPHLLVA
                     GTTGSGKSVGVNAMILSMLYKAQPEDVRFIMIDPKMLELSVYEGIPHLLTEVVTDMKD
                     AANALRWCVNEMERRYKLMSALGVRNLAGYNEKIAEADRMMRPIPDPYWKPGDSMDAQ
                     HPVLKKEPYIVVLVDEFADLMMTVGKKVEELIARLAQKARAAGIHLVLATQRPSVDVI
                     TGLIKANIPTRIAFTVSSKIDSRTILDQAGAESLLGMGDMLYSGPNSTLPVRVHGAFV
                     RDQEVHAVVQDWKARGRPQYVDGITSDSESEGGAGGFDGAEELDPLFDQAVQFVTEKR
                     KASISGVQRQFRIGYNRAARIIEQMEAQGIVSEQGHNGNREVLAPPPFD"
     gene            22234..22845
                     /gene="lolA"
                     /locus_tag="PN977_001557"
     CDS             22234..22845
                     /gene="lolA"
                     /locus_tag="PN977_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010354356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein chaperone LolA"
                     /protein_id="HDI6041603.1"
                     /translation="MKKIAITCALLSSLVASSVWADAASDLKSRLDKVSSFHASFTQK
                     VTDGSGAAVQEGQGDLWVKRPNLFNWHMTQPDESILVSDGKTLWFYNPFVEQATATWL
                     KDATGNTPFMLIARNQSSDWQQYNIKQNGDDFVLTPKASNGNLKQFTINVGRDGTIHQ
                     FSAVEQDDQRSSYQLKSQQNGAVDAAKFTFTPPQGVTVDDQRK"
     gene            22856..24199
                     /gene="rarA"
                     /locus_tag="PN977_001558"
                     /gene_synonym="mgsA"
     CDS             22856..24199
                     /gene="rarA"
                     /locus_tag="PN977_001558"
                     /gene_synonym="mgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein
                     RarA"
                     /protein_id="HDI6041604.1"
                     /translation="MSNLSLDFSDNTFQPLAARMRPENLAQYIGQQHLLAAGKPLPRA
                     IEAGHLHSMILWGPPGTGKTTLAEVIARYANADVERISAVTSGVKEIREAIERARQNR
                     NAGRRTILIVDEVHRFNKSQQDAFLPHIEDGTITFIGATTENPSFELNSALLSRARVY
                     LLKSLSTEDIEQVLTQAMEDKTRGYGGQDIVLPDETRRAIAELVNGDARRALNTLEMM
                     ADMAEVDDSGKRVLKPELLTEIAGERSARFDNKGDRFYDLISALHKSVRGSAPDAALY
                     WYARIITAGGDPLYVARRCLAIASEDVGNADPRAMQVAIAAWDCFTRVGPAEGERAIA
                     QAIVYLACAPKSNAVYTAFKAALADARERPDYDVPVHLRNAPTKLMKEMGYGQEYRYA
                     HDEANAYAAGEVYFPPEIAQTRYYFPTNRGLEGKIGEKLAWLAEQDQNSPIKRYR"
     gene            24290..25582
                     /gene="serS"
                     /locus_tag="PN977_001559"
     CDS             24290..25582
                     /gene="serS"
                     /locus_tag="PN977_001559"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HDI6041605.1"
                     /translation="MLDPNLLRNEPDAVAEKLARRGFKLDVDKLGALEERRKVLQVKT
                     ENLQAERNSRSKSIGQAKARGEDIEPLRLEVNKLGEELDAAKAELDALQAEIRDIALT
                     IPNLPADEVPVGKDENDNVEVSRWGTPREFDFEVRDHVTLGEMHSGLDFAAAVKLTGS
                     RFVVMKGQIARMHRALSQFMLDLHTEQHGYSENYVPYLVNQDTLYGTGQLPKFAGDLF
                     HTRPLEEEADTSNYALIPTAEVPLTNLVRGEIIDEDDLPIKMTAHTPCFRSEAGSYGR
                     DTRGLIRMHQFDKVEMVQIVRPEDSMAALEEMTGHAEKVLQLLGLPYRKIILCTGDMG
                     FGACKTYDLEVWIPAQNTYREISSCSNVWDFQARRMQARCRSKSDKKTRLVHTLNGSG
                     LAVGRTLVAVMENYQQADGRIEVPEVLRPYMNGLEYIG"
     gene            25821..28265
                     /gene="dmsA"
                     /locus_tag="PN977_001560"
     CDS             25821..28265
                     /gene="dmsA"
                     /locus_tag="PN977_001560"
                     /EC_number="1.8.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415414.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit A"
                     /protein_id="HDI6041606.1"
                     /translation="MKTKIPDAVLAAEVSRRGLVKTTAIGGLAMASSALTLPFSRIAH
                     AVDSALPAKSDEKVIWSACTVNCGSRCPLRMHVVDGEIKYVETDNTGDDNYDGLHQVR
                     ACLRGRSMRRRVYNPDRLKYPMKRVGARGEGKFERISWEEAYDIIATNMQRLIKEYGN
                     ESIYLNYGTGTLGGTMTRSWPPGNTLVARLMNCCGGYLNHYGDYSSAQIAEGLNYTYG
                     GWADGNSPSDIENSKLVVLFGNNPGETRMSGGGVTYYLEQARQKSNARMIIIDPRYTD
                     TGAGREDEWIPIRPGTDAALVNGLAYVMITENLVDQAFLDKYCVGYDEKTLPASAPKN
                     GHYKAYILGEGPDGVAKTPEWASQITGVPADKIIKLAREIGSTKPAFISQGWGPQRHA
                     NGEIATRAISMLAILTGNVGINGGNSGAREGSYSLPFVRMPTLENPIQTSISMFMWTD
                     AIERGPEMTALRDGVRGKDKLDVPIKMIWNYAGNCLINQHSEINRTHEILQDDKKCEL
                     IVVIDCHMTSSAKYADILLPDCTASEQMDFALDASCGNMSYVIFNDQVIKPRFECKTI
                     YEMTSELAKRLGVEQQFTEGRTQEEWMRHLYAQSREAIPELPTFEEFRKQGIFKKRDP
                     QGHHVAYKAFREDPQANPLTTPSGKIEIYSQALADIAATWELPEGDVIDPLPIYTPGF
                     ESYQDPLNKQYPLQLTGFHYKSRVHSTYGNVDVLKAACRQEMWINPLDAQKRGINNGD
                     KVRIFNDRGEVHIEAKVTPRMMPGVVALGEGAWYDPDTKRVDKGGCINVLTTQRPSPL
                     AKGNPSHTNLVQVEKV"
     gene            28276..28893
                     /gene="dmsB"
                     /locus_tag="PN977_001561"
     CDS             28276..28893
                     /gene="dmsB"
                     /locus_tag="PN977_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit B"
                     /protein_id="HDI6041607.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDYKDLTPEVSFRRIYEYAG
                     GDWQEDNGVWHQNVFAYYLSISCNHCEDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNETKGRMTKCDGCYDRVAEGKKPICVESCPLRALDFGPIDELRK
                     KHGDLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPKEV"
     gene            28895..29758
                     /gene="dmsC"
                     /locus_tag="PN977_001562"
     CDS             28895..29758
                     /gene="dmsC"
                     /locus_tag="PN977_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit
                     DmsC"
                     /protein_id="HDI6041608.1"
                     /translation="MGSGWHEWPLMIFTVFGQCVAGGFIVLALALLKGDLRAEAQQRV
                     IACMFGLWVLMGIGFIASMLHLGSPMRAFNSLNRVGASALSNEIASGSIFFAVGGIGW
                     LLAMLKKLSPALRTLWLVVTMVLGVIFVWMMVRVYNSIDTVPTWYSIWTPMGFFLTMF
                     MGGPLLGYLLLSLAGVDGWAMRLLPAISVLALVVSGVMSVMQGAELATIHSSVQQAAA
                     LVPDYGALMSWRIVLLAVALCLWIAPQLKGYQPAVPLLSVSFILLLAGELIGRGVFYG
                     LHMTVGMTVAS"
     gene            complement(29794..30420)
                     /locus_tag="PN977_001563"
     CDS             complement(29794..30420)
                     /locus_tag="PN977_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HDI6041609.1"
                     /translation="MTKPYVRLDKNDAAVLLVDHQAGLLSLVRDIEPDKFKNNVLALG
                     DLAKYFNLPTILTTSFETGPNGPLVPELKAQFPDAPYIARPGNINAWDNEDFVKAVKA
                     TGKKQLIIAGVVTEVCVAFPALSAIEEGFDVFVVTDASGTFNEITRHSAWDRMSQAGA
                     QLMTWFGVACELHRDWRNDIEGLATLFSNHIPDYRNLMTSYDTLTKQK"
     gene            30735..31883
                     /locus_tag="PN977_001564"
     CDS             30735..31883
                     /locus_tag="PN977_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDI6041610.1"
                     /translation="MSTYTRPVMLLLSGLLLLTLAIAVLNTLVPLWLAQEHMSTWQVG
                     VVSSSYFTGNLVGTLLTGYVIKRIGFNRSYYLASFIFAAGCAGLGLMIGFWSWLAWRF
                     VAGVGCAMIWVVVESALMCSGTSRNRGRLLAAYMMVYYVGTFLGQLLVSKVSTELMSV
                     LPWVTGLTLAGILPLLFTRVLNQQAENHDSTSITSMLKLRQARLGVNGCIISGIVLGS
                     LYGLMPLYLNHKGVSNASIGFWMAVLVSAGILGQWPIGRLADKFGRLLVLRVQVFVVI
                     LGSIAMLSQAAMAPALFILGAAGFTLYPVAMAWACEKVEHHQLVAMNQALLLSYTVGS
                     LLGPSFTAMLMQNFSDNLLFIMIASVSFIYLLMLLRNAGHTPKPVAHV"
     gene            32093..33523
                     /locus_tag="PN977_001565"
     CDS             32093..33523
                     /locus_tag="PN977_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415419.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDI6041611.1"
                     /translation="MAGNVQEKQLRWYNIALMSFITVWGFGNVVNNYANQGLVVVFSW
                     VFIFALYFTPYALIVGQLGSTFKDGKGGVSTWIKHTMGPGLAYLAAWTYWVVHIPYLA
                     QKPQAILIALGWAMKGDGSLIKEYSVVALQGLTLVLFIFFMWVASRGMKSLKIVGSVA
                     GIAMFVMSLLYVAMAVTAPAITEVHIATTNITWETFIPHIDFTYITTISMLVFAVGGA
                     EKISPYVNQTRNPGKEFPKGMLCLAVMVAVCAILGSLAMGMMFDSRNIPDDLMTNGQY
                     YAFQKLGEYYNMGNTLMVIYAIANTLGQVAALVFSIDAPLKVLLGDADSKYIPASLCR
                     TNASGTPVNGYFLTLVLVAILIMLPTLGIGDMNNLYKWLLNLNSVVMPLRYLWVFVAF
                     IAVVRLAQKYKPEYVFIRNRPLAMTVGIWCFTFTAFACLTGIFPKMEAFTAEWTFQLA
                     LNVATPFVLVGLGLIFPLLARKANSK"
     gene            complement(33524..33634)
                     /locus_tag="PN977_001566"
                     /pseudo
     CDS             complement(33524..33634)
                     /locus_tag="PN977_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415420.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            complement(33733..34473)
                     /gene="pflA"
                     /locus_tag="PN977_001567"
     CDS             complement(33733..34473)
                     /gene="pflA"
                     /locus_tag="PN977_001567"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase 1-activating protein"
                     /protein_id="HDI6041612.1"
                     /translation="MSVIGRIHSFESCGTVDGPGIRFITFFQGCLMRCLYCHNRDTWD
                     THGGKEVTVEDLMKEVVTYRHFMNASGGGVTASGGEAILQAEFVRDWFRACKKEGIHT
                     CLDTNGFVRRYDPVIDELLEVTDLVMLDLKQMNDEIHQNLVGVSNHRTLEFAKYLANK
                     DVKVWIRYVVVPGWSDDDDSAHRLGEFTRDMGNVEKIELLPYHELGKHKWVAMGEEYK
                     LDGVKPPKKETMERVKSILEQYGHKVMF"
     gene            complement(34665..36947)
                     /gene="pflB"
                     /locus_tag="PN977_001568"
     CDS             complement(34665..36947)
                     /gene="pflB"
                     /locus_tag="PN977_001568"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HDI6041613.1"
                     /translation="MSELNEKLATAWEGFTKGDWQNEVNVRDFIQKNYTPYEGDESFL
                     AGATEATTTLWDKVMEGVKLENRTHAPVDFDTAVASTITSHDAGYINKALEKVVGLQT
                     EAPLKRALIPFGGIKMIEGSCKAYNRELDPMIKKIFTEYRKTHNQGVFDVYTPDILRC
                     RKSGVLTGLPDAYGRGRIIGDYRRVALYGIDYLMKDKYAQFTSLQADLENGVNLEQTI
                     RLREEIAEQHRALGQMKEMAAKYGYDISGPATNAQEAIQWTYFGYLAAVKSQNGAAMS
                     FGRTSTFLDVYIERDLKAGKITEQEAQEMVDHLVMKLRMVRFLRTPEYDELFSGDPIW
                     ATESIGGMGLDGRTLVTKNSFRFLNTLYTMGPSPEPNMTILWSEKLPLNFKKFAAKVS
                     IDTSSLQYENDDLMRPDFNNDDYAIACCVSPMIVGKQMQFFGARANLAKTMLYAINGG
                     VDEKLKMQVGPKSEPIKGDLLNYDEVMERMDHFMDWLAKQYITALNIIHYMHDKYSYE
                     ASLMALHDRDVIRTMACGIAGLSVAADSLSAIKYAKVKPIRDEDGLAIDFEIEGEYPQ
                     FGNNDPRVDDLAVDLVERFMKKIQKLHTYRDAIPTQSVLTITSNVVYGKKTGNTPDGR
                     RAGAPFGPGANPMHGRDQKGAVASLTSVAKLPFAYAKDGISYTFSIVPNALGKDDEVR
                     KTNLAGLMDGYFHHEASIEGGQHLNVNVMNREMLLDAMENPEKYPQLTIRVSGYAVRF
                     NSLTKEQQQDVITRTFTQSM"
     gene            complement(37002..37859)
                     /gene="focA"
                     /locus_tag="PN977_001569"
     CDS             complement(37002..37859)
                     /gene="focA"
                     /locus_tag="PN977_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter FocA"
                     /protein_id="HDI6041614.1"
                     /translation="MKADNPFDLLLPAAMAKVAEEAGVYKATKHPLKTFYLAITAGVF
                     ISIAFVFYITATTGTGTMPFGMAKLVGGICFSLGLILCVVCGADLFTSTVLIVVAKAS
                     GRITWGQLAKNWLNVYFGNLVGALLFVLLMWLSGEYMTANGQWGLNVLQTADHKVHHT
                     FIEAVCLGILANLMVCLAVWMSYSGRSLMDKAFIMVLPVAMFVASGFEHSIANMFMIP
                     MGIVIRDFASPEFWTAVGSAPENFSHLTVMNFITDNLIPVTIGNIIGGGLLVGLTYWV
                     IYLRENDHH"
     gene            complement(38265..40025)
                     /locus_tag="PN977_001570"
     CDS             complement(38265..40025)
                     /locus_tag="PN977_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     accessory protein YcaO"
                     /protein_id="HDI6041615.1"
                     /translation="MTQTFIPGKDAALEDSIARFQQKLSDLGFQIEEASWLNPVPNVW
                     SVHIRDKECALCFTNGKGATKKAALASALGEYFERLSTNYFFADFWLGETIANGPFVH
                     YPNEKWFPLTENDDVPEGLLDDRLRAFYDPENELTGSMLIDLQSGNEDRGICGLPFTR
                     QSDNQTIYIPMNIIGNLYVSNGMSAGNTRNEARVQGLSEVFERYVKNRIIAESISLPE
                     IPADVLARYPAVVEAIETLEAEGFPIFAYDGSLGGQYPVICVVLFNPANGTCFASFGA
                     HPDFGVALERTVTELLQGRGLKDLDVFTPPTFDDEEVAEHTNLETHFIDSSGLISWDL
                     FKQDADYPFVDWNFSGTTEEEFATLMAIFNKEDKEVYIADYEHLGVYACRIIVPGMSD
                     IYPAEDLWLANNSMGSHLRETILSLPGSVWEKEDYLNLIEQLDEEGFDDFTRVRELLG
                     LATGPDNGWYTLRIGELKAMLALAGGDLEQALVWTEWTMEFNSSVFSPERANYYRCLQ
                     TLLLLAQEEDRQPLQYLNAFVRMYGADAVEAASAAMSGEAAFYGLQPVDSDLHAFAAH
                     QSLLKAYEKLQRAKAAFWAK"
     gene            40155..40847
                     /locus_tag="PN977_001571"
     CDS             40155..40847
                     /locus_tag="PN977_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HDI6041616.1"
                     /translation="MKAFDLHRMAFDKVPFDFLGEVALRSLYTFVLVFLFLKMTGRRG
                     VRQMSLFEVLIILTLGSAAGDVAFYDDVPMVPVLIVFITLALLYRLVMWLMAHSEKLE
                     DLLEGKPVVIIEDGELAWSKLNNSNMTEFEFFMELRLRGVEQLGQVRLAILETNGQIS
                     VYFFEDDKVKPGLLILPSDCTQRYKVVPESADYACIRCSEIIHMNAGEKQLCPRCANP
                     EWTKASRAKRVT"
     gene            41046..>41896
                     /gene="serC"
                     /locus_tag="PN977_001572"
     CDS             41046..>41896
                     /gene="serC"
                     /locus_tag="PN977_001572"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HDI6041617.1"
                     /translation="MAQIFNFSSGPAMLPAEVLKQAQQELRDWNGLGTSVMEVSHRGK
                     EFIQVAEEAEKDFRDLLNVPSNYKVLFCHGGGRGQFAAVPLNILGDKTTADYVDAGYW
                     AASAIKEAKKYCTPNVFDAKVTVDGLRAVKPMREWQLSDNAAYMHYCPNETIDGIAID
                     ETPDFGKDVVVAADFSSTILSRPIDVSRYGVIYAGAQKNIGPAGLTIVIVREDLLGKA
                     NIACPSILDYSILNDNGSMFNTPPTFAWYLSGLVFKWLKANGGVAEMDKINQQKAELL
                     YGVIDNS"
BASE COUNT        10414 a        10201 c        11072 g        10209 t
ORIGIN      
        1 cagggggcgg cggcgattgg ccgccccctg cgcgctcctt gcgtcagtgg caatatgtgg
       61 cttagctcat gaaaggagcg caacaagatg atgaatcaac atataacaac attttaaaaa
      121 aagcctgaca ttgcgccagg ccttctgcgt taattaatta cgcttttcca gcagggtccg
      181 gtaaatcaga ccaccgataa taccgccgac aattggcacc acccagaaga accacagttg
      241 ttctaacgcc cagccgccct ggaagatagc aaccgcggtg ctgcgcgccg ggttaacaga
      301 agtgttagtc accggaatac tgattaagtg aatcagggtt aaggccagac caatagctat
      361 cggcgcaaaa cctgccggag cgaatttgtc ggttgcgccg tggatcacta acaggaagcc
      421 cgcactcaat accagttcaa ctaccagcgc ggaaagcatg gaataaccgc ctggcgaatg
      481 ctcgccataa ccgttagaag caaaaccgct ggctgccgcg tcaaaacctg ttttaccact
      541 ggcaattaaa tacagcagcg ccgccgcaac aataccgccg acaacctggg caattacgta
      601 gccaacgact tcttttgccg ggaaacgtcc gccagcccat aaaccaatag tgaccgccgg
      661 gttaaaatga ccaccagaaa tatgaccaac agcaaaggcc atcgtcagaa cggtcagacc
      721 gaatgcaagc gccacgccgg caaaaccaat gcctaattcc gggaagcctg cggccagtac
      781 ggcactacca cagccaccaa aaacaagcca gaaagtacca aaacattcag ctgctaattt
      841 tctgaacata tccacctcaa ttaaaaattg accctgtgaa aaatatggtc gttttatagg
      901 gtcgtcgtaa aaaagtgacg acggaaataa tgcgcggcta ttttaaaaac gaaggcgagt
      961 cattcaccag ataaataaat ccagtaaatt tgatttaggg caacagcggg ttgccccata
     1021 tagtcatttg tctgattgac agtgtagtgc aagcaaaaga tttaatcctt taggcgtaat
     1081 aaaaaataat ttatcatgct aattatttga ttttgttgtt tttgcaggct tatcagcaag
     1141 agggagtata acgcgattat tcgctcattt ttcagacatt tgccatgctt aaatgtgatg
     1201 tcatcacgta ttagcatggc ctttcccgtt atactgccag cgtaaaggat aagtcacata
     1261 tttctggagg ggatatgatt cttgagcgcg ttgaaattgt gggttttcgc ggtatcaacc
     1321 gtttgtcgtt gatgctggaa caaaacaacg tcctgattgg ggagaacgcg tggggtaaat
     1381 ccagcttgct ggacgcctta accctgctgc tatcgccaga atcagatctc taccattttg
     1441 agcgcgacga tttctggttc ccgccgggag atatcaacgg acgggaacat catttgcata
     1501 ttattttgac cttccgcgaa tcgctgccag gccgacatcg ggtacgccgc tatcggccac
     1561 tggaagcgtg ctggacgcca tgcaccgatg gctatcaccg tattttttat cgtctggaag
     1621 gggagagtgc ggaagacggc agcgtgatga cactccgcag ttttctcgat aaagacggac
     1681 atccgattga tgtcgaggat attaacgatc aggcacgcca tctggtgcgt ttaatgccgg
     1741 tgctgcgctt gcgtgatgcc cgttttatgc gccgtattcg taacggcacg gtgccaaatg
     1801 tccctaatgt ggaagtcacc gcgcgccagc tcgatttcct cgcccgtgag ttatcctcac
     1861 atccgcaaaa tctctctgat gggcagattc gtcagggact ttccgcaatg gtacagctgc
     1921 ttgagcatta tttctctgag cagggggccg gacaggcgcg atatcgttta atgcggcggc
     1981 gagccagcaa tgagcaacga agctggcgct atctggatat catcaaccgg atgattgacc
     2041 gacctggagg gcgctcgtat cgggttattt tgctcggcct gtttgctact ttgttgcagg
     2101 caaaaggcac attgcgactg gataaagacg cccgtccatt gttgctgatc gaagatccag
     2161 aaacccgttt acaccccatt atgctttcag ttgcctggca tctgttgaat cttctgccat
     2221 tgcagcgcat cgctactacc aactcgggag agttgctttc gttaacgccg gtagagcatg
     2281 tttgccgact ggtacgtgag tcctcgcgcg ttgccgcctg gcgtctgggg ccgagtggtt
     2341 tgagtaccga agatagccga cgcatctctt tccacattcg ttttaatcgt ccatcatcgc
     2401 tgtttgcacg ctgctggctg ctggtggaag gggaaacaga aacctgggtt atcaacgaac
     2461 tggcgcgcca gtgtggacac catttcgatg ccgaagggat taaggtcatt gaatttgccc
     2521 agtccgggct aaaaccactg gtgaaatttg cccggcgaat ggggattgaa tggcatgtac
     2581 tggtcgatgg cgatgaagca gggaagaaat atgccgctac ggtacgcagc ctgttgaata
     2641 acgatcggga agccgaacga gaacatttaa cggcgttacc ggcgctggat atggaacatt
     2701 ttatgtatcg ccagggattt tccgatgtgt tccaccgcgt ggcgcaaatc ccggaaaatg
     2761 taccgatgaa tctgcgcaaa attatctcga aagcgatcca tcgctcttcc aaacccgatc
     2821 ttgccattga agtggcaatg gaggccggac gtcgtggtgt ggattccgta ccgacgctgc
     2881 tgaaaaaaat gttctcacgc gtgctgtggc tggcgcgcgg tcgcgcggat taaccgcgaa
     2941 acatcgtggc catttgtggc tgaatagcgt cgagcatctc atagcgccga cggtattcag
     3001 cccgtttttt actggcgatt tcggcaatct cttttcgtgc tatctgtgct ggaaggcggt
     3061 aatggcgttc agcatcacat acgccgccaa ccgattccca gaaagcgttg taatcagcgt
     3121 ggatcttgcc ttctttatcg cgataacgca ggctgcggta aatatgcgtt tcattgctga
     3181 cggcaataat ctgctctacc tgcaaacgtt gggcaaacag acaggccgct tccatcacga
     3241 ggcgtttggg aaatagcccg tggcaggctt tcgtcgcatt ctggatttcc tgatgtggga
     3301 tttcccattt tgcgccttgc agtccgccaa taaacattgt tcttttcccc tgatattcac
     3361 acagggtaaa cgtgatctct gccagaggaa taccttcgct gttgcgaaac aggattgtgc
     3421 tgtcaccttc tttatccatt gagatcatca tggtcagctc aagtgtgaac tgctcgccgt
     3481 ttttgccttc cagcttcgcc agttgcagcc cgggggtatt caaatataag ctgaattctt
     3541 ccgccgacat acacccgcgg agtaacgcat aatggtaacg taacgcctcc agcaattgct
     3601 tacggctaag attcgccgca aggtaagggc gatgcagacg cacaggcagt cgcggctggc
     3661 gcgttaacaa cacattgaga ttaggccagt gggaaagttc gttcatccac tcaacgctta
     3721 aacgcggcat aatcaacgag cgcagcaaaa atttctggcg aaaactacgg cgatgccaga
     3781 atttacccgg ccgacactgt ccacgtgcca gactaagaaa aagtgacagg ctgctgagag
     3841 attcagatgg cgtaaaggtc cgttcagtta gctgcgacat attcatgaaa tcaatggtta
     3901 tacatgacgt cgatttcacc attgcgtatc ttaaccaaac atcaatagtg tgattactaa
     3961 cgtaaatttt agggttttgt tgatatttcg ttgaagttaa tgacccggat tggcatatgg
     4021 agtattcaga atatttatga aaaagcggaa aaccgtgaag aagcgttacg ttattgcgct
     4081 ggtgatagtc atcgccggac tgattacgtt atggagaatt cttaacgcac ccgttccgac
     4141 ttatcagacg ctgattgtgc gccccggtga tttacagcaa agcgtgctgg cgaccggaaa
     4201 gctggacgcg ctgcgtaagg ttgacgtggg cgcgcaggtc agcggtcagt tgaaaacgct
     4261 gtcggtggcg attggcgata aagtaaaaaa agaccagctt ttaggggtta ttgatcctga
     4321 acaggctgaa aaccagatca aggaggtgga agcaacgctg atggagctac gcgcgcagcg
     4381 gcagcaggcg gaagcggagc tgaaactggc gcgggtgacg tattcccgtc agcaacgtct
     4441 tgcacaaacg caagcggttt cacagcagga tctcgatacc gccgcgacgg agatggctgt
     4501 gaaacaggcg caaattggca ccattgacgc gcaaatcaag cgcaatcagg cctcactcga
     4561 tacggctaaa accaatctcg attacacccg catcgttgcc ccgatggccg gggaagtcac
     4621 gcaaatcacc actctgcaag gccagacggt gattgccgca caacaagcac cgaacattct
     4681 gacgctggca gatatgagca ccatgctggt aaaagcgcag gtttctgaag cggatgtaat
     4741 ccacctgaag ccggggcaaa aagcctggtt tacggtacta ggcgatccgc tgacgcgcta
     4801 tgaggggcaa atcaaggatg tgctaccgac gccggaaaag gttaacgacg ctattttcta
     4861 ttacgcccgt tttgaagtcc ccaaccccaa tggtttgctg cggctggata tgaccgcgca
     4921 agtgcatatt cagctcaccg atgtgaaaaa tgtgctgacg atccctctgt tggcgttagg
     4981 cgatccggtt ggcgataatc gttataaagt caaattgttg cgtaatggtg aaacacgcga
     5041 gcgtgaagtg acgattggcg cacgtaacga taccgatgtt gagattgtca aagggctgga
     5101 agcgggcgat gaagtggtga ttggtgaggc caaaccagga gctgcacaat gacgcctttg
     5161 ctcgaattaa aggatattcg tcgcagctat cctgccggtg atgagcaggt tgaggtgctg
     5221 aagggcatca gcctcgatat ttatgccggt gagatggtag cgattgttgg cgcttcgggt
     5281 tccggcaaat cgaccctgat gaatattctc ggctgcctgg ataaggccac cagcggcacc
     5341 tatcgcgtcg ccggtcagga tgttgccacg ctggacgccg atgcgctggc gcaactgcgc
     5401 cgcgagcatt tcggctttat tttccagcgt taccatttgc tttcgcattt aaccgccgag
     5461 cagaacgttg aagttcccgc cgtctatgct ggtcttgagc ggaaacagcg gctgcttcgc
     5521 gcccaggagt tgctgcaacg actggggctg gaagaccgta cagagtatta tccggcacag
     5581 ctttcgggtg gtcagcaaca gcgtgtcagc atcgcgcggg cattgatgaa cggcggtcag
     5641 gtaattcttg ccgatgaacc aaccggtgca ctggacagcc attctggcga agaggtgatg
     5701 gcgatcctgc atcagctgcg cgatcgcggg catacggtga ttatcgtcac ccacgatccg
     5761 caggttgctg ctcaggccga gcgggtgatc gaaattcgtg acggcgaaat tgtgcacaat
     5821 cctcccgccg ttgaaaaagt gaatgctact ggcgggacgg aacctgttgt caacacggcg
     5881 tctggctggc ggcagtttgt cagcggtttt aacgaggcgc tgacgatggc atggcgggcg
     5941 ctggcagcga ataaaatgcg tactttactg accatgctgg ggattattat cggtattgcg
     6001 tcggtggttt ccattgtcgt ggtgggcgac gccgccaaac aaatggtgct ggcggatatt
     6061 cgttctattg gtacgaatac tattgatgtc tatcccggga aagattatgg cgatgacgat
     6121 ccgcaatatc agcaggcgct gaagtacgac gacttaatcg ccatccaaaa acaaccgtgg
     6181 gtcgcctcag ccacacctgc cgtctcgcaa aacctgcgcc tgcgttataa caatgttgat
     6241 gtggctgcca gtgccaatgg cgtgagcggc gattatttta atgtctatgg catgaccttc
     6301 agtgaaggga acacctttaa tcaggagcag ctgaacggtc gtgcgcaggt ggtggttctc
     6361 gacagtaata ctcgccgcca gcttttcccc cataaagcag atgtggttgg cgaggtgatt
     6421 ctggtcggca atatgcccgc cagagtcatt ggtgtggcgg aagaaaaaca gtcgatgttt
     6481 ggtagcagta aagtgctgcg tgtctggcta ccttacagca cgatgtccgg gcgagttatg
     6541 ggccagtcgt ggctgaactc cattactgtc agggtgaaag aaggatttga cagcgccgag
     6601 gcggaacagc aactcacgcg tttactttca ctgcgccacg gtaagaagga tttctttacc
     6661 tggaacatgg atggtgtctt gaaaaccgtt gaaaagacca cacgtacttt acaactgttt
     6721 ctgacgctgg tggcggtgat ttcgctggtg gtgggcggta ttggtgtaat gaatattatg
     6781 ctggtgtcag tgaccgagcg gacgcgggaa attggcattc gcatggctgt aggtgcgcga
     6841 gcaagcgatg ttttgcaaca gttcctgatc gaagccgtac tggtttgcct ggtcggtggc
     6901 gcgttgggaa taacactgtc actgttaatt gctttcacct tgcagctttt cttacccggc
     6961 tgggagattg gtttttcacc gttggcactg ctgctggcgt ttctctgctc gacggtcacc
     7021 gggattttat ttggctggtt acccgcacga aatgcggcac gactggatcc agtagatgct
     7081 ctggcacgag agtaattttt gagataaaaa tgccagccga tcgggctggc attttgcctt
     7141 taggatgtac acaatgagac agaagagcta tgcgactgcc gcttctactt cgacgggcac
     7201 aataacactg gcgtgattgc cttttggccc ctggtggaca tcaaactgaa cggattgtcc
     7261 agcttttagc gttctgtaac catccatctg aatggtggaa taatgagcga aaatatcttc
     7321 gccgccgcct tcagggcaga tgaaaccaaa ccctttggca ttgttgaacc acttaacagt
     7381 acccttttcc atgcttcgac atccttcgca aatcttatac aagtaagatg gaataaaccg
     7441 gggtcagaga gggggctgtt caaaacctcg ccaactctag aaatacaatt tagagaatta
     7501 gggcgagccg tcaagcattt gacaggggac agggggcagg tatgaatcaa aaatttgaag
     7561 cagttaacgc tattgacagg aatgtgacag atgtcgctga tgccaacgat agatgatagt
     7621 tatctatcat gtggagtaga ttggtcaggc aaataagctc ttgtcagcgg cagggcgttc
     7681 tgccgataac cgtaaccgaa gatgataact gacaatgggt aaaacgaacg actggctgga
     7741 ctttgatcaa ctggcggaag aaaaagttcg cgacgcgcta aaaccgccat ctatgtataa
     7801 agtgatatta gtcaatgatg attacactcc gatggagttt gttattgacg tgttacaaaa
     7861 attcttttct tatgatgtag aacgtgcaac gcaattgatg ctcgctgttc actaccaggg
     7921 gaaggccatt tgcggagtct ttaccgccga ggttgcagaa accaaagtgg cgatggtgaa
     7981 caagtacgcg agggagaatg agcatccatt gctgtgtacg ctagaaaaag cctgaatgca
     8041 ggcataaaaa ttgggggagg tgcctatgct caatcaagaa ctggaactca gtttaaatat
     8101 ggctttcgcc agagcgcgcg agcaccgtca tgagtttatg accgtcgagc acttgttact
     8161 ggcgctgctc agtaacccat ctgcccggga ggcgctggaa gcgtgttctg tggatttggt
     8221 tgcgctccgt caggaactgg aagcctttat tgaacaaacc acacccgttc tgcctgccag
     8281 tgaagaggag cgcgacacac agccgacgct gagttttcag cgtgtactgc aacgtgcggt
     8341 cttccatgtc cagtcctccg gtcgcaatga ggttaccggt gcaaacgttc tggtcgctat
     8401 ttttagcgaa caggagtcgc aggcggcata tctgttgcgt aaacatgaag tcagccgtct
     8461 cgatgtggtg aactttatct ctcatggcac gcgtaaagac gagccgacac agtcttctga
     8521 tcctggcagc cagccaaaca gcgaagaaca agctggtggg gaggaacgta tggagaattt
     8581 cacgacgaac ctgaatcagc ttgcgcgagt gggcggaatc gacccactga ttggtcgtga
     8641 gaaggagctg gagcgtgcta ttcaggttct ctgccgtcgc cgtaaaaaca acccgctgct
     8701 ggtgggggaa tctggtgtcg gtaaaaccgc gattgcggaa ggtcttgcct ggcgaattgt
     8761 tcagggcgat gtgccggaag tgatggctga ctgtacgatt tactctctcg atatcggttc
     8821 tctgttagcg ggcactaaat atcgcggcga ctttgaaaaa cgttttaaag cgttgctcaa
     8881 gcagctggag caggacacta acagcatcct gtttattgat gagatccaca ccattatcgg
     8941 tgcgggtgca gcgtctggtg gtcaggtcga tgcggctaac ctgatcaaac cgttgctctc
     9001 cagcggtaaa attcgcgtaa ttggttcgac aacctatcag gagttcagca acattttcga
     9061 gaaagaccgt gctctggcgc gtcgcttcca gaaaattgat attactgaac cgtcgatcga
     9121 agaaactgtt caaatcatca atggcctgaa accgaagtac gaagcgcacc atgacgtgcg
     9181 ttataccgca aaagcggtgc gtgcagcggt agagctggcg gtgaaataca ttaacgatcg
     9241 tcatctgccg gataaagcca ttgacgttat cgacgaagcg ggcgctcgcg cacgcctgat
     9301 gccggtaagc aaacgcaaga aaaccgttaa tgtggcggat attgagtctg tggtggcccg
     9361 tattgcgcgc attccagaga agagtgtttc gcagagtgac cgcgataccc tgaaaaacct
     9421 cggcgatcgc ctgaaaatgc tggtcttcgg tcaggataaa gccattgagg cgctgactga
     9481 agccattaag atggcgcgtg caggtttagg tcacgaacat aaaccggtcg gttcgttcct
     9541 gtttgccggt cctaccgggg tcgggaaaac agaggtgacg gtacagcttt cgaaagcttt
     9601 gggcattgag cttctgcgct ttgatatgtc cgagtatatg gaacgccata ccgtcagccg
     9661 tctgattggt gcgcctccgg gatacgttgg ttttgatcag ggggggctgc tgaccgatgc
     9721 ggtcatcaag catccgcacg cggtactgct gctggacgaa atcgagaaag cgcatccgga
     9781 cgtgttcaat attctgttgc aggtgatgga caacggtacg ctgaccgata acaacggacg
     9841 caaagcggac ttccgtaacg tggtattggt gatgaccacc aacgctgggg tacgagaaac
     9901 tgagcgtaaa tcgattggtc ttatccatca ggacaacagc accgatgcga tggaagagat
     9961 caagaagatc tttacgccgg agtttcgtaa ccgtctcgac aacattatct ggttcgatca
    10021 tctctccacc gacgtgatcc atcaggtagt ggataaattc atcgtcgagt tgcaggttca
    10081 gctggatcag aaaggtgttt ctctggaagt gagccaggaa gcgcgtaact ggctggccga
    10141 gaaaggttac gaccgggcaa tgggcgcacg tccgatggcg cgtgtcatcc aggacaacct
    10201 gaaaaaaccg ctcgccaacg aactgttgtt tggttcgctg gtggacggcg gtcaggtgac
    10261 ggttgcgctg gataaagaga aaaatgagct gacttacgga ttccagagtg cacaaaagca
    10321 caaggcggaa gcagcgcatt aatctgattg tctggtaggt tggtgaagtc cgtaatctcg
    10381 aaagaggtta cggacttttt gtttgtaggg tggaggaggt tcagaccctt tttttgatga
    10441 tgatggtaag ttgttgataa ttagtgctgc gggaaggtaa ggataaaaaa gggtggtagc
    10501 aggaaaatga gatggttttg ctttattaat aacggactaa acgtgtagta tttgagctca
    10561 ctgccgtaca ggcagataaa atgcgaaaaa aagcccgtac tttcgtacga gctcttcttt
    10621 aaatatggcg gtgagggggg gattcgaacc cccgatacgt tgccgtatac acactttcca
    10681 ggcgtgctcc ttcagccact cggacacctc accaaattgt tttgctgcca aacctcatgg
    10741 gtggcaacgg ggcgctacta tagggagttg gagtaaaacg gtcaagaaga attttaatga
    10801 taattattgt ttgctcatac tgtaaacaag ttgtgcagta tatctacatc gagacaagtt
    10861 atggacttat acttccaaag tacttcatac atatcacaaa ataaaaaggt cggttaaacc
    10921 gaccttttac tcgttctttc tcttcgccca tcaggcggta aaacaatcag cgactacgga
    10981 agacaatgcg gcctttgctc aggtcgtacg gggtcagttc aacagtcact ttgtcgcccg
    11041 tcaggatgcg gatgtagttt ttgcgcattt taccggagat gtgtgcagta accacgtgac
    11101 cgttttctaa ctctacgcgg aacatggtat taggcaacgt ttcaagaacg gtaccttgca
    11161 tttcaatatt gtcttctttg gccatctaat cctctggggt atcactaccg taatttgaac
    11221 cggcaagata atgccgaagt tctgtaaata agtaaagatt tgcgcgctaa atcgcaacaa
    11281 acaggttcgg cacattactc cgaaaacaca cggctaagcc gcaccaaaag cgcaacgtat
    11341 aagggagcgg tgagataaac gatgggcgtt acctgacgcg aaaaattcct tatcggcagc
    11401 ggggtaatga gcgtaaccaa ctctgcgacc gcaattataa cactctgggg agaaatgtgc
    11461 cgaaaacatt cattcttgtg gtgaaaacaa gcaccgtggt acccagaaat tattcggcaa
    11521 tcgtccgagg cgcatttgat tgagataatt tagataatcc cggcggggaa tttcgcaggc
    11581 accaagcgaa gctgtgtgat cgttaaggac ctggcagtcg ataagcttac cgccatgacc
    11641 aataaattcc tcacagaata ccagaagcgc cgttttagac gcattttcca tccggctgaa
    11701 catggactcg ccacaaaata gcgttccctg ggccacgccg tacataccgc cgacaagctc
    11761 atcttcacgc cagacttcaa tagagtgggc atgaccgagt tcgtgaaggc gatggtaggc
    11821 ttcgaccacg ccacgcgtga tccaggttcc ttcttcgcga tcgctggcac agccttcaat
    11881 gacctgacca aaagcgtaat tcatcgtgac tcgatagggc gagcgtttat gaaatcgctt
    11941 catactacgg ctgatatgca gtgattctgg ccatagcacc gcgcggggat cgggcgacca
    12001 ccagaggatg gggtcgcctg gagaaaacca cggaaaaata ccacgctggt aagccattaa
    12061 caggcgcgca gggctaagat cgccccccag tgccagcagg ccgttaggct cacgtaatgc
    12121 gccttccggg gaagggaagg ctattgaatg gcgagaaagc tgaaccaggc gcatgaccgc
    12181 aaaactccac gcaagtcgga tcgttcaata atagcttaca aaccctgctt aaactggtaa
    12241 taacgcccct gtcgggcaag cagttctgcg tgagtacctt gctcaataat ttgcccgttg
    12301 tccatcacta ttatttgctg gaaacgagag agcccgcgaa gtcgatgggt gaccattaac
    12361 accgttttct cacgcatcat ttctgcaagt aattcaagga tctgactttc agttgtggca
    12421 tctaagcctt cggtaggttc atccagcaat accagtggcg catcatgtaa cagcgcacgg
    12481 gcgatagcca gacggcgcag ttcaccaccg gagagctggc gtccgccttc acccaaccaa
    12541 ctgttgagac ctgcatcctc gagcagcttc tccagaccaa cgcgacgcaa gatctccgcc
    12601 agagcctcat cactactatc aggcgaggcg agtaacagat tatcacgcag cgtggcgcta
    12661 aacagatgca ctcgctgtgg cacaacgctg atggtctgtc gtagagtcgc ttcattcagg
    12721 ctggctatgg ggctatcgtt aagcaaaatc tcgccctgtt gcgggtccca tgcgcgggtc
    12781 agcagttgta acaatgttga tttgccgcat ccggttcgcc cgagaatcgc tatatgttct
    12841 ccggcgttta cctgaagaga aatcccttta agtgcctgtt gggattgctc tggataagtg
    12901 aactgaacat cccgtaatgt cagcgaaaca cgatcggcaa cacgagtttg ggtatcaggg
    12961 aaggtgactt ccggtttttg atccgttaag tcagtgatcc gtacggcaga ggcaatgact
    13021 tgccccagat gctgaaatgc acccgttact ggtgccagtg cttcaaacgc ggctaacgcg
    13081 cagaagacaa acagggcaat taacgcgccg ggttgagcat tgccgccaac gccgccagac
    13141 gccatccaca gcatcaggat caccgctaac gcgccaatga gcagcattat cgcttgcgac
    13201 aatgcggtga gttcagattg acgacgttgc gattccagcc actgaatttc ggtattctct
    13261 aactgcgtgc gataacgatc gctggcacca aaaatggtca gctcagcttg cccttgcagc
    13321 caggccgtca gttgttggcg atactgcccg cgaagatgag tcagattttg cccggtgctt
    13381 tttcccgcac gataaaacag tggtggcata aggaaaagcg tcagtaacat aatgccgccc
    13441 agcgtaaagg cgagggtgaa atcaaggaaa cttaacccga ttgtcaccac cataatcacc
    13501 acaaaagcgc ccaccagcgg cgagataacg cgcaggtaaa gatgatcgag cgtatcaaca
    13561 tccgccacca cgcggttgag caattcgccc tgacgatagc gcgccagtcc ggcaggggag
    13621 aggggcagca atttgctgaa ggtataaatg cgcagatgct gcaacacgcg gaaagtcgcg
    13681 tcgtgactta ccaggcgttc aaaatagcgc ccggcagtac gggtgattgc tgcgccacgc
    13741 acgcccgcag cgggtagcat atagttgaag ctgtacagtc cggcaacccc cgcaaccgct
    13801 gaggcggaga ggaaccagcc cgaaagtgtc aacagaccga tgctggcgag cagcgtgaca
    13861 attgccagca caataccaag acttaacatc catttatgac gtttatacag tgccagatag
    13921 ggtagcaaag cgcgcattta aatctcctcc tgacgatggg ccagcaatgt ggcgaaagga
    13981 ccaccagcca cgcttaattc cgcgtaacgt ccttgctcaa taatctgacc atcctgcatt
    14041 acccaaatga catcccagtc agcaagatct tctaactggt gggtgaccat taacgttgtc
    14101 tggcgcagag aggcggcatt aagcgcctcc attacgcgct gttcactgtg agcatcaagg
    14161 ctggcagcgg gttcatccaa cagtaatagc gaacagggat tcagtaatgc tcgggccacc
    14221 gccacgcgct gcgcctgccc cacggaaagg cgggcagcct ggtcaccaac aggcgtatca
    14281 acgccttgtg gcaggagcgg tagaaactca ctgacccagg cgttatccag tgctgcttgt
    14341 aactcgtgtt cgttggcatc aggtcgcgcc agtagtacgt tatcccgtag tgttgctgcc
    14401 ggtaattgtg ggttttgccc aacccaggag agatgtttac gccatgattc tggtgataaa
    14461 tcgcgtaatt ctatcccgtt gattcgtagc gatccctgat atgagagaaa accagaaagc
    14521 gcgttcagca gtgagctttt acctgaaccg ctgcgaccaa ccaacactgc acgttggcct
    14581 gctggcaaag taaagttcag cggtccggcc agcgttttac cttctggtga tgtaataaac
    14641 agctcctcgg cttcaatggt caccggatcg gtcgatgcta attccgcctc accacgctgc
    14701 ggatgggcaa gcggggtttc cataaacgtt ttcaaactgt cagctgcacc aacagcctgg
    14761 gctttagcat gataaaacgt accgagatcg cgtaatggct ggaaaaactc cggcgcaagg
    14821 atcagggcca gaaaacccgc agccagcgtc acgccagtat catagtgacc aaaatccagc
    14881 tcgccgagat aggaaaaacc aaagtagacc gccaccagag caattgacag cgaggtaaaa
    14941 aattcgagaa tgccggagga taaaaacgcc agccgtagca cttccattgt ccgttggcgg
    15001 aaatcttccg aagcagaacg aatactttca atttcagctt caccacgacc aaaaatacgc
    15061 aatgtttcca tgccgcgcag gcgatcgaga aaatgcccac ttaagcgagc aagagcgaga
    15121 aagttacgtc ggttagcatc ggcagccccc attccaacca gcgccataaa caacggaatt
    15181 aacggagcag tgcccagcag aatgagcgcc gcagcccagt tagaggggaa gatagccacc
    15241 acaatcagca acggcaccga cactgccagc gccatttgcg gcaggtagcg tgcatagtaa
    15301 tcatgcatat cgtcaatttg ctcgagtacc agcgtcgccc agctccccgc cggtttaccc
    15361 tgaatccacg ctggccctgc ttgttgcaga cggtcgagaa cctgacggcg gatggcaaag
    15421 cggatatgct gcccggcgtg ataacccacc cgttcgcgta accagaccac ccatgcgcgc
    15481 agtacaaagg tcagaaccag taacgtaaag ggaagcagca gggcttcacg gggaatattc
    15541 tccataatca tatgttgcag aattcgcgcc atgaaccagg cctgggcaat gatcaatatg
    15601 ccgctcacaa agcccagcag acgagaaata ttcagccaac gttgggagat gacgctttgc
    15661 tgttttaacc agcgggttaa ctctttttga cgggatttat tcattgcacg cttagcaggt
    15721 gagttatcag aattatttgc agagcaatgt tacaacgagg aaaaaataaa ggcgacccat
    15781 agtcgcatgg tgtcgccttc tttacttttg ttactgattt gtaaaattat tttgcgtcag
    15841 ctaaaccatc gaggtagcgt tccgcatcaa gtgctgccat gcagcctgta ccagcagatg
    15901 taatagcctg gcgataaatg tgatccatca cgtcgcctgc ggcaaagacg ccagggatgc
    15961 tggtctgggt ggcattacca tgaatacccg actgtacttt gatgtagccg ttttccagtt
    16021 ccagctgccc ttcgaaaata gccgtattcg ggctgtgacc gatagcaaca aacagaccgg
    16081 caacgtcgag tgactcgatg ttatcgctgt tttgcgtatc gcgcagacga acgccagtga
    16141 cgcccatctg atcgccggtc acctcttcca gcgtacggtt ggtgtgtaga atgatgttgc
    16201 cgttctccac tttatccatc agacgcttaa taaggatttt ttccgcgcgg aaaccgtcac
    16261 ggcggtgaat cagatgcact tccgaagcga tgttagacag atacagcgcc tcttcaaccg
    16321 cggtattgcc gccgccgatg accgcaactt tctggttgcg atagaagaaa ccgtcgcagg
    16381 tagcacaagc agaaacccca cggcctttaa acgcttcctc agaaggaagg ccgagatagc
    16441 gtgcagaagc tccggtggca ataatcagcg cgtcgcaagt gtattcgccg ctatcgccag
    16501 tcagacggaa cggacgattc tgcaaatcaa ctttgttgat gtgatcaaag atgatttcag
    16561 tttcaaattt ggtggcatgt tcgtgcatgc gctccattaa taacggaccg gtcagatcgt
    16621 ttggatcgcc aggccagttt tccacttccg tggtggtggt cagttggccg cctttttcca
    16681 tgccggtaat cagcacaggt tgcaggttgg cgcgcgccgc gtagacagca gcggtgtatc
    16741 ccgccgggcc tgaacccagg ataagcagtt tactgtgttt ggtcgtgccc atgagatccc
    16801 catagttgtt ggcagacaat gggcaggatt gtagggaatt tacagacgta aaaaaagagt
    16861 atgacgattt tgttaacaat ttgtgcaatc ggcagcatcg ataagcaggt caaattctcc
    16921 cgtcattatc acctctgcta cttaaatttc ccgctttata agccgattaa atgatgaata
    16981 aacgcccctg ttaatgaata tctggcatgt tgtactaaaa atcgatgttt tgctttgaca
    17041 atcccctggt gttttgcgaa aacattctag gaagaaaaaa aacagtattc ttatatgcgc
    17101 ataatcatgc atgtaaatac catgtttacc gtgctagtga aatctacgta tggcgtggac
    17161 agacgccatt cgtgatgtcg atagctgcca caaggcaacg gtcttctcac cgtagaccca
    17221 ggcattgcgc gccgtgaatc ttcatgattt cggtctatcg tgacgggtag cgactctgaa
    17281 cagtgatgtt tcagggtcag acaggagtag ggaaggaata cagagagaca ataataatgg
    17341 tagatagcaa gaagcgccct ggcaaagatc tcgaccgtat cgatcgtaac attcttaatg
    17401 agttgcaaaa ggatgggcgt atttctaacg tcgagctttc taaacgtgtg ggactttccc
    17461 caacgccgtg ccttgagcgt gtgcgtcggc tggaaagaca agggtttatt cagggctata
    17521 cggcgctgct gaacccccat tatctggatg catcacttct ggtattcgtt gagattactc
    17581 tgaatcgtgg cgcaccggat gtgtttgaac aattcaatac cgctgtacaa aaacttgaag
    17641 aaattcagga gtgtcattta gtgtccggtg atttcgacta cctgttgaaa acacgcgtgc
    17701 cggatatgtc agcttaccgt aagttgctgg gggaaaccct gctgcgtctg cctggcgtca
    17761 atgacacacg gacatacgtt gttatggaag aagtcaagca gagtaatcgt ctggttatta
    17821 agacgcgcta acacggaaca ggtgcaaaat cggcgtattt tgattacact cctgttaatc
    17881 catacagcaa cagtactggg gtaacctggt actgttgtcc gttttagcat cgggcaggaa
    17941 aagcctgtaa cctggagagc ctttcttgag ccaggaatac actgaagaca aagaagtcac
    18001 attgacaaag ttaagtagcg gccgccgcct tctggaggcg ttgctgatcc ttattgtcct
    18061 gtttgccgtc tggttgatgg ctgccttact aagctttaac ccttcggacc ccagctggtc
    18121 gcaaacggcc tggcatgaac ctatccataa tttaggtggg atgcccggtg catggctggc
    18181 agatacgctg ttctttattt ttggagtgat ggcttacacc attcccgtca ttattgtcgg
    18241 cggttgttgg tttgcctggc gtcatcagtc cagcgacgaa tacattgatt attttgccgt
    18301 ttcgctacgc atcattggcg ttttggcgct catccttacc tcctgtggtc tggcggcaat
    18361 caacgctgac gatatctggt attttgcctc cggtggcgtc attggcagct tactgagcac
    18421 tacgctacaa ccactgctac acagtagcgg gggaactatt gcactgctct gcgtttgggc
    18481 agcgggcctg acgctgttca ccggttggtc atgggtgacc attgctgaaa aactcggcgg
    18541 ctggatttta aatattctta ccttcgccag taatcgtacc cgtcgcgatg atacctgggt
    18601 cgatgaagat gagtatgaag acgacgaaga atatgaagaa gatgaaagtc acggcaaaca
    18661 gcatgaatca cgccgtgccc gtattcttcg cggcgcgcta gcgcgtcgta aacggttggc
    18721 ggaaaaattc attaatccga tggggcggca aacagacgct gcgttgttct ccggtaagcg
    18781 gatggatgat gacgaagaga ttacctacac tgcacgcggt gtggctgccg accctgacga
    18841 cgtcctcttt tcgggcaatc gtgcaacgca gccagaatat gacgagtacg atccattatt
    18901 aaacggtgcg ccaattaccg aacctgttgc tgtagcagct gctgctacca cggcgacaca
    18961 aagctgggct gcgccggttg aacctgtgac tcagacgccg cctgttgcct ctgttgatgt
    19021 tccacctgca caacctacag tagcctggca gcctgtaccg gggccacaaa cgggagagcc
    19081 ggttattgct cctgcaccgg aaggttaccc acagcagcca caatatgcgc agcctgcagt
    19141 gcaatataat gagccgctgc aacaaccagt acagccacag cagccgtatt atgcacctgc
    19201 agctgaacaa tctgcgcaac aaccttacta cgctcctgcg ccggaacagt ctgcgcaaca
    19261 gccgtattac gcccctgcgc cagaacaatc ggtggcaggt aacgcctggc aagccgaaga
    19321 gcagcaatcc acttttgctc cacagtctac ataccagacg gagcaaactt atcagcagcc
    19381 agccgctcag gagccgttgt accaacagcc gcaacccgtt gaacagcagc ctgttgtgga
    19441 gcctgaaccc gttgtagaag agacaaaacc cgcgcgtccg ccgctttact attttgaaga
    19501 agtggaagag aagcgtgccc gtgaacgtga acaacttgcg gcctggtatc aaccgattcc
    19561 agaacctgtt aaagaaccag aaccgatcaa atcttcgcta aaagcacctt ctgttgcagc
    19621 agtacctcca gtggaagccg ctgccgccgt ttctccgctg gcatctggcg tgaaaaaagc
    19681 gacactggcg acgggggctg ccgcaaccgt tgccgcgcca gtcttcagtc tggcaaatag
    19741 cggtggaccg cgtccacagg tcaaagaggg gattggcccc cagttgccac gaccgaaacg
    19801 catccgcgtg ccaactcgtc gtgaactggc gtcttacggt attaagctgc cctcacagcg
    19861 tgcggcagaa gaaaaagccc gtgaagccca gcgcaatcag tatgattctg gcgatcagta
    19921 taacgatgat gaaatcgatg cgatgcagca ggatgaactg gcgcgtcaat tcgcccagac
    19981 acagcagcaa cgctacggcg aacagtatca acatgatgtg cccgtgaacg cagaagatgc
    20041 agatgctgcg gcagaggctg aactggctcg tcagtttgct caaactcaac aacaacgtta
    20101 ttccggcgaa caaccggctg gggcgaatcc gttcactctg gatgattttg aattttcgcc
    20161 aatgaaagcg ttgctggatg atggtccaca cgaaccgctg tttacgccaa ttgttgaacc
    20221 tgtacagcag ccgcaacaac cgattgcacc gcagcaacag tatcagcagc cgcagcaacc
    20281 agttgcgccg cagccgcagt atcagcagcc gcaacagccg gttgcaccgc agcagcaata
    20341 tcagcagccg caacagccgg ttgcaccaca gccgcaatat cagcagccgc aacagccagt
    20401 tgcgccgcag ccgcagtatc agcagccaca acaaccggtt gcgccgcagc agcaatatca
    20461 gcagccacaa cagccagttg cgccgcagcc gcagtatcag cagccacaac agccagttgc
    20521 gccacagccg caggataccc tgcttcatcc gctgttgatg cgtaatggcg acagccgtcc
    20581 gttgcataaa ccgacgacgc cgctgccttc tctggatttg ttgacgccgc cgccgagcga
    20641 agtggagccg gtagatacct ttgcgcttga acaaatggcg cgtctggtgg aagcgcgtct
    20701 ggctgatttc cgtattaaag ccgatgtcgt caattactct ccggggccag ttatcactcg
    20761 ctttgaactt aacctggcac ctggcgtaaa agcggcgcgc atttctaact tgtcacggga
    20821 ccttgcccgt tcactttcga cggtggcggt gcgtgtcgtt gaagttattc ctggcaaacc
    20881 ctatgtaggt ctggagttac cgaataaaaa acgacaaacc gtttatctgc gcgaagtttt
    20941 ggataacgcc aaattccgcg ataatccgtc gccattaacc gtggtgctgg gtaaagatat
    21001 cgccggtgag ccggtggttg ccgatctggc aaaaatgccg cacttgttgg ttgcggggac
    21061 taccggttcc ggtaagtctg tcggggtgaa cgcgatgatc ctgagcatgc tttataaagc
    21121 acagccagaa gatgtgcgtt tcatcatgat cgacccgaaa atgctggagc tttcggttta
    21181 tgaaggcatt ccgcatctgt taacggaagt tgttactgat atgaaagatg ccgccaacgc
    21241 gctgcgctgg tgtgttaacg agatggagcg tcgttataaa ctgatgtctg cgctgggtgt
    21301 gcgtaatctg gcgggttata acgaaaaaat tgctgaagcc gatcgcatga tgcgtccgat
    21361 tccagacccg tactggaagc cgggtgacag tatggatgcc cagcatccgg tgctgaaaaa
    21421 agaaccgtac attgtggtgt tggtggacga atttgccgac ctgatgatga cggtaggtaa
    21481 aaaagtggaa gagcttatag cacgtctggc gcaaaaagcc cgtgccgcgg gtatccacct
    21541 cgtactggca acgcagcgtc catcggttga tgttattact ggtctgatta aagcgaatat
    21601 tccgacccgt atcgccttta ccgtatccag taagattgac tcacgtacca ttcttgatca
    21661 ggctggcgcg gaatcactgc tgggtatggg ggatatgctt tactctgggc cgaactccac
    21721 gttgccggta cgtgttcatg gtgcttttgt tcgcgatcag gaagttcatg ccgtggtgca
    21781 ggactggaaa gcgcgtggtc gcccacagta tgttgatggc atcacctccg acagtgaaag
    21841 cgaaggtggc gcgggcggtt tcgatggcgc tgaagaactg gatccgttgt tcgatcaggc
    21901 ggtgcagttt gtcactgaaa aacgcaaagc atcaatttct ggcgtacagc gtcagttccg
    21961 cattggttat aaccgtgcag cgcgtattat cgaacagatg gaagcgcagg ggattgtcag
    22021 cgaacagggg cacaacggta atcgtgaagt gctggcccca ccgccgtttg actaattaat
    22081 gcatcgtatg ccggaaaagg cgcggtagcg tcgcatccgg cactctatca actgaaaatt
    22141 cagtattttc ttctttcctc aagctgatta ttagcctgga atagagagta gagggagctc
    22201 ccggtcggga gtgacgtaat ttgaggaata atgatgaaaa aaattgctat cacctgtgca
    22261 ttactctcaa gcttagtagc aagcagcgtt tgggctgatg ccgcaagcga tctgaaaagc
    22321 cgcctggata aagtcagcag cttccacgcc agcttcacac aaaaagtgac tgacggtagc
    22381 ggcgcggcgg tgcaggaagg tcagggcgat ctgtgggtga aacgtccaaa cttattcaac
    22441 tggcatatga ctcaacctga cgaaagcatt ctggtttctg acggtaaaac actgtggttc
    22501 tataacccgt tcgttgagca agctacggca acctggctga aagatgccac cggtaatacg
    22561 ccgtttatgc tgattgcccg caaccagtcc agcgactggc agcagtacaa tatcaaacag
    22621 aatggcgatg actttgtcct gacgccgaaa gccagcaatg gcaatctgaa gcagttcacc
    22681 attaacgtgg gacgtgatgg cacaatccat cagtttagcg cggtggagca ggacgatcag
    22741 cgcagcagtt atcagctgaa atcccagcaa aatggggctg tggatgcagc gaaatttacc
    22801 ttcaccccgc cgcaaggcgt cacggtagat gatcaacgta agtagaggca cctgagtgag
    22861 caatctgtcg ctcgattttt cggataatac ttttcaacct ctggccgcgc gtatgcggcc
    22921 agaaaattta gcacagtata tcggccagca acatttgctg gctgcgggga agccgttgcc
    22981 gcgcgctatc gaagccgggc atttgcattc tatgatcctc tgggggccgc cgggtaccgg
    23041 caaaacaacc ctcgctgaag tgattgcccg ctatgcgaac gctgatgtgg aacgtatttc
    23101 tgccgtcacc tctggtgtga aagagattcg cgaggcgatc gagcgcgctc ggcaaaaccg
    23161 caatgcaggt cgccgcacta ttcttattgt tgacgaagtt caccgtttca acaaaagcca
    23221 gcaggatgca tttctgccac atattgaaga cggcaccatc acttttattg gcgcaaccac
    23281 tgaaaacccg tcgtttgagc ttaattcggc actgctttcc cgtgcccgtg tctatctgtt
    23341 gaaatccctg agtacagagg atattgagca agtactgact caggcgatgg aagacaaaac
    23401 ccgtggctat ggtggtcagg atattgttct gccagatgaa acacgacgcg ccattgctga
    23461 actggtgaat ggcgacgcgc gccgggcgtt aaatacgctg gaaatgatgg cggatatggc
    23521 cgaagtcgat gatagcggta agcgggtcct gaaacctgaa ttactgaccg aaatcgccgg
    23581 tgaacgtagc gcccgctttg ataacaaagg cgatcgcttt tacgatctga tttccgcatt
    23641 gcataagtcg gtacgtggta gcgcacccga tgcggcgctg tactggtatg cgcgaattat
    23701 taccgccggt ggcgatccgt tatatgttgc gcgtcgttgc ctggcaattg cttccgaaga
    23761 tgtcggcaat gccgacccac gggcgatgca ggtggcaatt gcggcctggg attgctttac
    23821 tcgcgttggc ccggcggaag gtgaacgcgc cattgctcag gcgattgttt acctggcctg
    23881 cgcgccaaaa agcaacgctg tctacaccgc gtttaaagcc gcgctggccg atgctcgcga
    23941 acgtcccgat tatgacgtgc cggttcattt gcgtaatgcg ccgacgaaat taatgaagga
    24001 aatgggctac gggcaggaat atcgttacgc tcatgatgaa gcaaacgctt atgctgccgg
    24061 tgaggtttat ttcccgccgg aaatagcaca aacacgctat tatttcccga caaacagggg
    24121 ccttgaaggc aagattggcg aaaagctcgc ctggctggct gaacaggatc aaaatagccc
    24181 cataaaacgc taccgttaat gttatcgttg cggtaatgtt gttactgtat ccctgtggtc
    24241 gcaggctgtg gccacatctc ccatttaatt cgataagcac aggataagca tgctcgatcc
    24301 caatctgctg cgtaatgagc cagacgcagt cgctgaaaaa ctggcacgcc ggggctttaa
    24361 gctggatgta gataagctgg gcgctcttga agagcgtcgt aaagtattgc aggtcaaaac
    24421 ggaaaacctg caagcggagc gtaactcccg atcgaaatcc attggccagg cgaaagcgcg
    24481 cggggaagat atcgagcctt tacgtctgga agtgaacaaa ctgggcgaag agctggatgc
    24541 agcaaaagcc gagctggatg ctttacaggc tgaaattcgc gatatcgcgc taaccatccc
    24601 taacctgcct gcagatgaag tgccggtagg taaagacgaa aatgacaacg ttgaagtcag
    24661 ccgctggggc accccgcgtg agtttgactt tgaagttcgt gaccatgtga cgctgggtga
    24721 aatgcactct ggcctcgact ttgcagccgc agttaagttg actggttccc gctttgtggt
    24781 aatgaaaggg cagattgctc gcatgcaccg cgcactgtcg cagtttatgc tggatctgca
    24841 taccgaacag catggctaca gtgagaacta tgttccgtac ctggttaacc aggacacgct
    24901 gtacggtacg gggcaactgc cgaaatttgc tggcgatctg ttccatactc gtccgctgga
    24961 agaagaagca gacaccagta actatgcgct gatcccaacg gcagaagttc cgctgaccaa
    25021 cctggtacgc ggtgaaatca tcgatgaaga tgatctgcca attaagatga ccgcccacac
    25081 cccatgtttc cgttctgaag ctggttcata tggtcgtgac actcgtggtc tgatccgtat
    25141 gcaccagttc gacaaagttg aaatggtgca gatcgtgcgc ccggaagact caatggcggc
    25201 gctggaagag atgaccggtc atgcggaaaa agtcctgcag ctgctgggcc tgccgtaccg
    25261 caaaatcatc ctttgcaccg gcgacatggg ctttggtgct tgcaaaactt acgacctgga
    25321 agtatggatc ccggcgcaaa acacctaccg cgagatctct tcatgctcta acgtctggga
    25381 tttccaggca cgtcgtatgc aggcacgctg ccgcagcaag tctgacaaga aaacccgtct
    25441 ggttcatacc ctgaacggtt ctggtctggc tgttggccgt acgctggttg cggtaatgga
    25501 aaactatcag caggctgatg gtcgtattga agtaccagaa gttctacgtc cgtatatgaa
    25561 cggactggaa tatattggct aatacccaat ttttctgaat ctaaaaagcg cctgcgggcg
    25621 ctttttttgt ctccctttga taccgaacaa taattactcc tcacttacac gtaatactac
    25681 tttcgagtga aaatctacct atctctttga ttttcaaatt attcgatata tacaagccta
    25741 tatagcgacc tgctatagaa ataattacac aatacggttt gttactggaa tcaatcgtga
    25801 gcaagcttga gtgagccatt atgaaaacga aaatccctga tgcggtattg gctgctgagg
    25861 tgagtcgccg tggtttggta aaaacgacag cgatcggcgg cctggcaatg gccagcagcg
    25921 cattaacatt accttttagt cggattgcgc acgctgttga tagtgccctt ccggcaaagt
    25981 cagacgaaaa ggtaatctgg agcgcctgta cagttaactg tggtagccgc tgcccgctac
    26041 gtatgcacgt tgtggacggt gaaattaaat atgttgaaac ggataatacc ggcgatgaca
    26101 actatgacgg cctgcaccag gtgcgcgcct gtctgcgtgg gcgttccatg cgtcgccgtg
    26161 tctacaatcc ggaccgcctg aaatatccga tgaaacgagt cggggcgcgc ggtgaaggca
    26221 aattcgagcg cattagctgg gaagaagcct acgacatcat cgcgaccaat atgcagcgcc
    26281 tgatcaaaga gtacggcaac gagtctatct atctgaacta tggtaccggt acgctgggcg
    26341 gcaccatgac ccgctcctgg ccgccgggaa ataccctggt cgctcgcctg atgaactgct
    26401 gcggcggtta tctgaaccat tacggtgact actcttccgc gcaaattgcg gaaggtttga
    26461 actataccta cggcggctgg gcagatggta acagcccgtc ggatatcgaa aacagtaaac
    26521 tggtagtgct gtttggtaat aaccctggcg aaacgcgaat gagtggcggt ggggtgactt
    26581 actatcttga acaggcgcgc cagaaatcta atgctcgcat gatcatcatc gatccgcgct
    26641 ataccgacac cggtgccggg cgcgaagatg agtggatccc tattcgtccg ggaacagatg
    26701 ccgcactggt taacggtctg gcgtatgtca tgatcaccga aaacctggtg gatcaggcat
    26761 tcctcgataa atattgcgtt ggctacgacg agaaaacact gccagccagt gcgccgaaaa
    26821 atggtcacta taaagcttat attctgggtg aagggccaga tggcgtggct aaaacgccgg
    26881 aatgggcctc gcaaatcact ggtgttccgg cagacaaaat catcaaattg gctcgtgaaa
    26941 tcggtagtac caaaccggcg tttatcagcc agggatgggg cccgcagcgt cacgctaacg
    27001 gtgaaatcgc aacccgtgct atctcgatgc tggcgattct gaccggtaac gttggtatta
    27061 acggaggcaa cagcggcgcg cgtgaaggtt catacagctt accgtttgtc cgtatgccga
    27121 ccctcgaaaa cccgatccag accagcattt cgatgtttat gtggaccgat gccattgaac
    27181 gtggcccgga aatgacagcg ctgcgtgatg gtgtgcgcgg gaaagataag ctggatgtgc
    27241 cgatcaaaat gatctggaac tatgccggta actgcctgat taatcagcat tctgaaatca
    27301 accgtaccca tgaaatcctt caggatgata agaagtgcga gctgattgtg gttatcgact
    27361 gccacatgac ctcatcggcg aaatatgctg acatcctgct gcctgactgc accgcttcag
    27421 aacagatgga ctttgcactg gatgcatcct gcgggaatat gtcttacgtg attttcaacg
    27481 atcaggtgat taaaccgcgc tttgaatgta agaccatcta tgaaatgacc agcgaactgg
    27541 caaaacgtct tggcgttgag caacaattta ctgagggccg tacccaggaa gagtggatgc
    27601 ggcatctgta tgcccagtcg cgggaagcga ttcctgaact gccaacgttt gaagagttcc
    27661 gtaagcaggg gatctttaaa aagcgcgacc cacaagggca tcacgttgct tataaagctt
    27721 tccgtgaaga tccgcaggca aatccactga ccacgccatc ggggaaaatt gagatttatt
    27781 cgcaggcgct ggctgacatt gccgcgacct gggaattgcc agaaggtgat gtgatcgatc
    27841 cactgccgat ctacacgccg ggctttgaaa gttatcagga tccgctgaac aaacagtatc
    27901 cgctgcagct tacgggtttc cactataaat ctcgcgttca ctcaacttac ggcaacgttg
    27961 atgtgctgaa agcagcttgc cgtcaggaaa tgtggatcaa cccgcttgat gcgcaaaaac
    28021 gcggtatcaa caacggcgat aaagtcagga tctttaacga tcgtggtgag gttcatattg
    28081 aggcgaaagt gacgccacga atgatgccgg gtgtggtcgc actgggtgaa ggtgcctggt
    28141 atgacccgga tacaaaacgt gtcgataagg gcggttgtat caacgtactg accactcaac
    28201 gtccgtctcc tctcgctaag gggaatccgt cacatacaaa ccttgttcag gttgaaaagg
    28261 tgtaaggagt aaccgatgac aacccagtat ggatttttta ttgattccag ccgttgcacc
    28321 ggttgcaaaa cctgcgagct ggcctgtaaa gactacaaag atttgacgcc agaagtcagt
    28381 ttccgccgca tttatgaata tgctggcggc gactggcagg aagataacgg tgtctggcac
    28441 cagaacgtgt ttgcctacta tctgtcgatt tcatgtaacc actgcgaaga tccggcttgt
    28501 actaaagtct gcccgagcgg tgcgatgcat aaacgtgaag atggttttgt tgtggtcgat
    28561 gaagatgtgt gcattggctg ccgctactgc catatggctt gcccgtatgg cgcaccgcaa
    28621 tataacgaaa cgaaaggccg tatgaccaaa tgcgatggtt gttatgaccg tgttgcggag
    28681 gggaaaaagc cgatctgtgt tgaatcttgc ccgctgcggg cgctggattt cggccctatc
    28741 gacgagctgc gtaaaaaaca tggcgatctg gcggccgttg cgccgttgcc gcgagctcac
    28801 tttaccaaac cgaatattgt gatcaaaccc aatgccaaca gccgcccgac cggggatacc
    28861 actggctatc tggcaaaccc gaaggaggtg tgagatggga agtggatggc atgaatggcc
    28921 gctgatgatc ttcacggtct tcgggcaatg tgtagcgggt ggttttatcg ttctggcttt
    28981 ggcgctgctc aaaggcgacc tgcgagcaga agcccagcag cgtgttattg cctgcatgtt
    29041 tggtttatgg gtgctgatgg gcattggctt tatcgcctct atgcttcatc ttggttcacc
    29101 gatgcgcgcc tttaactcgc tcaaccgggt aggggcttca gcactcagta acgaaatcgc
    29161 cagcggttcg atcttttttg ctgttggtgg catcggctgg ttactggcaa tgctgaaaaa
    29221 gctgtcaccg gcattgcgta cgctgtggct ggtggtgacg atggttcttg gcgtcatctt
    29281 tgtctggatg atggtgcgtg tctataacag catcgatacc gtacctacct ggtacagcat
    29341 ctggacgccg atgggcttct tcctgacgat gtttatgggc ggcccgttac tgggttacct
    29401 gttgttgagt ctggctggcg tcgatggctg ggcgatgcgt ctgctgccag caatttctgt
    29461 actggcactg gtagtgagtg gcgtgatgtc agtgatgcag ggcgcagagc tggcgactat
    29521 tcatagttct gtgcagcagg ccgcagcgct ggtgccggac tatggtgcgc tgatgtcctg
    29581 gcggatcgtg cttttggccg ttgccctgtg cttgtggatt gcaccacagc taaaaggtta
    29641 tcagcctgcg gttccgctac tttctgtatc attcattctg cttctggcag gggaattaat
    29701 cggtcgcggc gtattctatg gtttgcatat gaccgtgggg atgaccgtcg caagctaatc
    29761 agtaacaacc ggggtttcgg ccccggttct cttttatttc tgcttcgtta acgtgtcata
    29821 actggtcatc aaattacgat aatcaggaat gtggttagag aacaacgtcg ccagtccttc
    29881 aatatcatta cgccagtcac ggtgcagctc gcaagccacg ccgaaccatg tcatcaattg
    29941 cgcacctgct tgcgacatgc gatcccatgc cgaatgccgg gtaatttcat taaaggtccc
    30001 tgaggcgtca gtcacgacaa agacgtcaaa cccttcttca atggctgaaa gcgccgggaa
    30061 tgccacgcaa acttcagtta ccacaccggc aataattaac tgttttttac ctgtcgcttt
    30121 gactgctttt acaaaatctt cgttatccca ggcgttaata tttcccgggc gagcaatgta
    30181 aggtgcatcg gggaattgtg ctttcagttc tggaactaat ggaccgttag gaccggtttc
    30241 aaaactggtg gtaagaatag ttggtaagtt aaaatactta gccaggtcac ccagcgccag
    30301 cacgttattt ttaaacttat cgggttcgat atcccgtaca agggaaagta aaccagcctg
    30361 gtgatcaaca agcaaaacgg cagcatcatt tttatcaaga cgaacatacg gtttggtcat
    30421 cctcttctcc tttcgagagt cagtggtacg tctgaaatcg gtcagacgcg atgcattgct
    30481 ctgaaagcat agacgggaaa tatgagtttg ctgtgaccat gaaatttttc gactgaacga
    30541 agcgtatgaa atttgtgtta gatcaataaa aacaatcaga tgagattaat cgtctctcca
    30601 gcattagcgt tatgaatgag taatataaat cgataatacc tcagctgcga tatgcgcgta
    30661 ttttcaccca ttgacaatgt ttttggcggt ggcatgatgc gcatgaaatt tgaacttcct
    30721 cacggtttta attcatgtcc acgtataccc ggcctgtcat gcttttgctg tctggcctgc
    30781 ttttgttgac tctggcgatt gcggtgttaa atacactcgt gccgctttgg ctcgcccagg
    30841 aacacatgtc cacatggcag gtaggcgttg tcagctcatc ctattttacc ggcaacctgg
    30901 tcggtacatt gctgacaggg tatgtcatta agcgcattgg ctttaaccgc agctattatc
    30961 tggcctcctt catttttgcc gctggctgtg ccggccttgg cctgatgatt ggattctgga
    31021 gctggttggc ttggcgtttt gtcgccggcg tcggctgtgc catgatttgg gtggttgttg
    31081 agagcgcgct gatgtgcagt gggacgtcac gtaaccgtgg gcgtttgctt gctgcgtata
    31141 tgatggttta ttacgtggga acgtttttag gccagttact ggtcagcaaa gtttcaaccg
    31201 agctgatgtc cgtattgccg tgggttacag gtttgacgtt ggcagggatc ttaccgctgt
    31261 tgtttacgcg tgtgctgaat cagcaggctg aaaaccatga ttcgacgtca attacgtcaa
    31321 tgctaaaact ccgtcaggcg cggcttggcg tgaatggctg cattatctca ggaatcgttc
    31381 tgggatctct atatggcctg atgccgctgt acctcaatca caaaggggtg agcaatgcca
    31441 gcattggttt ctggatggcg gtactggtca gtgcgggtat ccttggacaa tggccgattg
    31501 gacgtctggc ggataagttt ggtcgactgc tggtgttgcg tgttcaggtc tttgtcgtca
    31561 ttctcggcag tatcgcgatg cttagccagg cggcgatggc cccagcgtta ttcatcctcg
    31621 gtgccgctgg ctttacgcta tatccggtgg cgatggcatg ggcttgcgag aaagttgaac
    31681 atcatcaact ggtggcgatg aaccaggcct tactgttgag ctataccgtg ggaagtctgc
    31741 ttggcccgtc atttactgcc atgctaatgc agaatttctc cgataattta ttgtttatca
    31801 tgatcgccag cgtatcgttt atctatttgc tgatgctgct tcgcaacgcc ggtcatacgc
    31861 cgaaacccgt tgctcacgtg taaatgaatt aacgcagagt gtgaacttac tgtttcacac
    31921 tctgtttttt tgtttcttct atctgacttg cttttttcaa aattttattc gtttcaaaat
    31981 aaaatgtgca gcaggttata attttgcatt tcgctatttc cgcacttctt atttgccgcg
    32041 cataatccct cgttttacag atgccccttt acttttggcg aaggatttgt ctatggctgg
    32101 gaatgttcag gaaaaacagc tgcgatggta caacattgcg ctgatgtctt ttatcactgt
    32161 ctggggtttt ggcaacgttg ttaataacta cgccaaccag gggctggtgg ttgttttttc
    32221 atgggtgttt atctttgcgc tctatttcac accttatgcg ctaattgttg gtcagttagg
    32281 ctcgaccttc aaagatggga agggcggggt cagtacctgg attaaacaca cgatgggacc
    32341 cggactggct tacctcgccg cgtggaccta ctgggtagtg catattccct atctggcaca
    32401 aaaaccccag gcaatactga ttgcgctcgg ttgggcgatg aaaggcgacg gttcgttaat
    32461 caaagaatat tcagtcgtag cgttacaggg gttaacgctg gtgctgttta tcttctttat
    32521 gtgggttgct tcacgcggta tgaaatcgct gaaaatcgtc ggttctgtgg cagggattgc
    32581 gatgtttgtt atgtcgctcc tgtatgtggc gatggcggta accgcgcctg cgattactga
    32641 agtgcatatt gcgaccacaa acattacctg ggaaacgttc attcctcata tcgactttac
    32701 ctacatcacc actatttcaa tgctggtttt cgcggttggc ggagcagaga agatttctcc
    32761 ttacgttaat caaacgcgca acccaggaaa agaatttcca aaagggatgt tatgcctggc
    32821 ggtgatggtt gcggtttgtg ccattctggg ctcgctggcg atggggatga tgtttgattc
    32881 gcgtaatatc ccggatgact taatgactaa cggtcagtat tacgcctttc agaaactggg
    32941 cgagtattac aacatgggta atactttaat ggtgatttac gccattgcga ataccctggg
    33001 acaagtagct gcgctggtat tctcgattga tgcgccgctt aaagtactat taggcgatgc
    33061 tgatagcaaa tatattccag ccagtttatg tcgtaccaac gcttctggta cgcccgttaa
    33121 tggctatttt ctgaccctgg tgctggtggc gatcctgatt atgctgccga cgctgggtat
    33181 cggcgatatg aataatctct acaagtggtt gctgaatctt aactcggtgg tgatgccact
    33241 acgttatctg tgggtatttg ttgcatttat tgcagtcgtt cgtctggcgc agaaatataa
    33301 accagagtat gtctttattc gtaacaggcc gctggcgatg actgtcggga tctggtgttt
    33361 tacctttacc gcttttgcct gcctgacagg gatcttcccg aaaatggaag ccttcactgc
    33421 agagtggacc ttccagttag cgctgaatgt tgcaacgccg tttgtcctgg ttggactggg
    33481 gctgatattc ccgctgctgg cgcgtaaagc gaatagtaaa taattattgt ggtcggtgac
    33541 gctgactctg gcagatctga cccgttttta cataatcgag aaatgcgcgc aagccgcagg
    33601 acatatactg gcggtttggg taatacattt ggaagctgtg ctgtaagggc gctagaaata
    33661 gagccctgaa ttatttatgc agcacggaat aaaaaagcgg caactcaata aagttgccgc
    33721 tttacgggga aattagaaca ttaccttatg accgtactgc tcaagaatgc ttttcacgcg
    33781 ttccatggtc tctttcttcg gtggtttaac accatcaagt ttgtattctt cacccatcgc
    33841 cacccatttg tgtttgccca gctcgtggta ggggagaagc tcgattttct caacgttgcc
    33901 catatcacgg gtaaattcac cgaggcgatg cgctgaatcg tcatcgtcag accagccagg
    33961 gacaacaacg tagcggatcc acaccttaac atctttgttc gccagatatt tagcgaactc
    34021 cagtgtacgg tggttagata cgccaaccag attctggtga atttcgtcgt tcatctgttt
    34081 gagatcgagc attaccaggt cggttacttc cagcagttca tcaatcaccg gatcgtaacg
    34141 acgaacaaaa ccgttggtat ccagacaggt atgaatgcct tcttttttgc aggcgcggaa
    34201 ccagtcacga acaaactcag cttgcaggat tgcttcaccg ccggatgcgg taacgccgcc
    34261 gccggaagcg ttcataaagt ggcgataggt caccacttcc ttcatcaaat cttcaacggt
    34321 aacttcttta ccgccatgcg tgtcccaggt gtcgcggtta tgacaataca ggcagcgcat
    34381 caggcagccc tggaaaaagg tgataaagcg aatacccggg ccgtctacgg ttccacagga
    34441 ttcaaaggag tgaatgcgac caataactga cattgcggtg tttctccaga tgtggcccat
    34501 ctgaggccgt gttggtgcgc agctcgaagg ctacgtcgag tctgttttgg cagtcacctt
    34561 aaagtataga tagctgacaa aaaaggctct cgtgctaaaa aaggccccac tttcgtggag
    34621 cctttattgt acgcttttta ctgtacgatt tcagtcaaat ctaattacat agattgagtg
    34681 aaggtacgag taataacgtc ctgctgctgt tctttagtca gcgagttgaa acgtactgcg
    34741 tagccagata cacggatggt cagctgcgga tatttttccg ggttttccat cgcgtcgagc
    34801 agcatttcac ggttcatcac gttaacgttc aggtgctgac caccttcgat ggacgcttcg
    34861 tggtggaagt aaccatccat cagaccagcc aggttggtct tacgaacttc gtcgtcttta
    34921 cccagtgcgt tcggaacgat agagaaggtg taggagatac catctttagc gtaagcaaac
    34981 ggcagtttag caacggaagt cagagaggct actgcacctt tctggtcacg accgtgcatc
    35041 gggttagcac ccggcccgaa cggcgcgcca gcacgacgac cgtctggggt gttaccagtt
    35101 ttcttaccat acacaacgtt agaagtgatg gtcagaacag actgagtcgg gatagcgtca
    35161 cggtaggtgt gcagtttctg aattttcttc atgaaacgtt ctaccaggtc aacagccagg
    35221 tcatctacac gcggatcgtt gttaccaaac tgcgggtatt cgccttcgat ttcgaagtcg
    35281 atagccagac cgtcttcgtc acgaatcggt ttaactttcg catatttgat tgcagacagg
    35341 gagtcagcag caacggacag accagcgata ccacacgcca tggtgcggat aacgtcacgg
    35401 tcgtgcagcg ccatcagaga ggcttcgtag ctgtacttgt cgtgcatgta gtggatgatg
    35461 ttcagtgcag tgatgtactg tttagccagc cagtccatga agtgatccat gcgctccatc
    35521 acttcatcgt agttcaggag atcgcctttg atcggttcag acttcggacc aacctgcatt
    35581 ttcagttttt cgtcaacgcc gccgttgatt gcgtacagca tggttttcgc caggtttgca
    35641 cgcgcaccga agaactgcat ttgtttacca acgatcatcg ggcttacgca gcaagcgata
    35701 gcgtagtcat cgttgttgaa gtccggacgc atcaggtcat cgttctcata ctgcagagaa
    35761 gaggtgtcga tggacacttt agcagcgaat ttcttgaagt tcagcggcag tttttcggac
    35821 cacagaatgg tcatgttcgg ttccggagac ggacccatgg tgtacagggt gttcaggaaa
    35881 cggaagctgt ttttggtaac cagggtacga ccgtcgaggc ccataccacc gatagattcg
    35941 gttgcccaga ttgggtcgcc agagaacagt tcatcgtatt ccggagtacg caggaagcga
    36001 accatacgca gtttcatgac caggtggtca accatttcct gcgcttcttg ttcggtgatc
    36061 ttgccagctt tcaggtcacg ttcgatgtac acatccagga aggtggaggt acgaccgaag
    36121 gacattgcag caccgttctg agacttaaca gcagccaggt agccgaagta agtccactgg
    36181 atagcttcct gagcgttggt agccggacca gagatgtcgt agccgtattt cgcagccatt
    36241 tctttcatct gacccagagc gcggtgctgt tcagcgattt cttcgcgcag acggatagtc
    36301 tgttccaggt ttacgccgtt ttccagatca gcctgcagag aggtgaactg agcgtatttg
    36361 tctttcatca ggtagtcgat accgtacagc gcaacgcgac ggtagtcacc gatgatacga
    36421 ccacggccat aagcatctgg cagaccggtc agaacaccgg atttacggca acgcaggatg
    36481 tccggagtgt aaacgtcgaa cacgccctgg ttgtgagttt tacggtattc agtgaagatt
    36541 tttttgatca tcgggtccag ttcgcggttg tacgctttgc aggaaccttc gatcattttg
    36601 ataccaccga acgggataag agcacgtttc agcggagctt cagtctgcag accaacaact
    36661 ttttccaacg ctttgttgat gtagccagcg tcgtgagagg tgatggtgga agcaacagcg
    36721 gtgtcaaagt caactggcgc gtgagtgcgg ttttccagtt taacgccttc cattactttg
    36781 tcccacaggg tggtggtcgc ttcagtagcg ccagccagga aggactcgtc accctcgtac
    36841 ggagtgtagt ttttctgaat gaagtcacgg acgtttactt cattctgcca gtcacctttg
    36901 gtaaaacctt cccaggctgt ggctaacttt tcattaagct cggacatgta acacctacct
    36961 tcttaagtgg attttttatt tactgcgtac ttcgacaacc attaatggtg atcgttttca
    37021 cgcaggtaaa tgacccagta tgtcaaccca accaacaaac caccgccgat aatattaccg
    37081 atcgtaaccg gaatcaggtt atcagtgatg aagttcatca cggtcaggtg agaaaaattt
    37141 tccggtgcag aaccgacagc ggtccagaat tccggagatg cgaagtcgcg gattacaata
    37201 cccatcggga tcataaacat gtttgcgata ctgtgctcaa aaccgctggc aacaaacatc
    37261 gcgaccggca gcaccataat gaacgcttta tccatcaggc tgcggccaga ataactcatc
    37321 catactgcca gacataccat caggtttgcc aggataccaa ggcagacggc ctcaataaaa
    37381 gtatggtgca ctttgtggtc ggcggtttgt aggacgttta gtccccattg accattcgcg
    37441 gtcatatact cgcctgaaag ccacattaaa agtacaaaca gcagtgcgcc gaccaggttg
    37501 ccaaaataga catttagcca gtttttcgcc aactgacccc aggtgatgcg cccactcgcc
    37561 ttagcaacaa caatcaacac tgtggaagta aagagatcgg ctccgcagac aacacaaaga
    37621 atcagcccca gagagaagca aatgccgcca accagttttg ccatgccgaa gggcattgtt
    37681 cctgtgccag tggttgctgt gatatagaag acgaatgcga ttgagatgaa aacaccggcg
    37741 gtaatcgcca gatagaaagt cttaagcgga tgtttcgttg ctttatagac acctgcctct
    37801 tcggccactt tggccattgc tgcaggaagt aaaagatcaa aagggttgtc agctttcaca
    37861 ctaactctct ctttattaag tcggcgacga gatactaaca aagcattata gatgagaaat
    37921 tgatatagat catatctcgc ctggcttata ggcccgtaac tcgcatggtt tttatgcaaa
    37981 tacggagtaa atatttgatt atccaaataa aaataaattt taaaaattaa caaatgagtt
    38041 gaattttttc cgcatcctcc gctaaaacag ttaattaaaa gggagcacca ggcgaataaa
    38101 gtaacaatat cgatcgtatt tattaaatac aaattaccga tacttaactt tataattaca
    38161 attattttat taatgcaaat atatgtaaag cggggcatta aaaaaacgcc ccgtaatata
    38221 actcagatga atcaaatagc ctacattgcg taggctattt gattttattt tgcccagaat
    38281 gccgctttgg cgcgctgcag cttttcgtag gctttcagca acgattgatg tgcagcaaac
    38341 gcgtgcagat cgctatctac tggttgcaag ccataaaacg ccgcttcgcc gctcattgcc
    38401 gcactggcgg cttccacggc gtctgcgcca tacatacgaa cgaaggcatt cagatattgc
    38461 agcggttggc ggtcttcttc ctgtgccagt aataacaacg tttgcagaca gcgataatag
    38521 ttggcgcgtt ccggactgaa cactgatgag ttaaattcca tcgtccattc agtccagacc
    38581 agagcctgtt ccagatcgcc accagccagc gccagcatgg cttttaattc accgatacgc
    38641 agggtgtacc aaccgttatc cggtccggtc gccagaccca gtagttcacg tacgcgagta
    38701 aagtcatcaa aaccttcttc atccagttgc tcgatgaggt tcaggtaatc ttctttttcc
    38761 cacacactgc ctggtagcga gagaatggtt tcacgtaaat ggctacccat actgttattc
    38821 gctaaccaca gatcttcagc cggataaata tcggacatgc caggcacgat aatacggcaa
    38881 gcataaacgc ccagatgctc gtaatcggca atataaactt ctttatcttc tttgttgaag
    38941 atagccatca gcgtagcaaa ctcttcttcc gtagtgccgg agaaattcca gtccacaaac
    39001 ggataatcgg catcctgctt gaacaggtcc caggagatta aaccgctgga atcgataaag
    39061 tgcgtttcga ggttggtatg ttcagcaact tcttcatcat cgaaggttgg cggagtaaac
    39121 acatccaaat ctttcaggcc acgaccttgc agcagctcgg tcacggtacg ttccagtgct
    39181 acgccaaaat caggatgcgc accgaaagag gcaaagcagg tgccgttagc aggattaaac
    39241 agaaccacgc aaatcaccgg atactggccg ccaagcgaac catcataagc aaagattggg
    39301 aaaccttctg cttccagtgt ttcgatcgct tcaaccaccg ctgggtaacg cgccagcacg
    39361 tctgccggga tttccggcaa gctgatgctt tcagcaataa tgcggttttt cacgtagcgt
    39421 tcgaaaactt cggacaatcc ctgaacgcgt gcttcgttac gggtattgcc agcagacata
    39481 ccgttagaaa cgtacaagtt accaatgata ttcatcggaa tataaatggt ctggttgtcg
    39541 gactggcgcg taaacggtag accacaaata ccacgatctt cgttaccgga ttgcaggtca
    39601 atcagcatac taccggtcag ttcattctcc ggatcgtaaa atgcgcgcag acggtcatcg
    39661 agcagccctt ctggtacatc gtcattttcg gtcagtggga accatttttc gttgggataa
    39721 tgcacgaatg ggccgttggc gatggtttcg cccagccaga agtccgcaaa aaagtagttg
    39781 gttgagagac gctcgaaata ttcaccgagt gcagaagcca gcgccgcttt tttggttgcg
    39841 cctttaccgt tggtaaaaca cagtgcgcac tctttgtcgc gaatatgtac agaccagacg
    39901 ttaggcacgg gattcagcca ggaggcctct tcaatctgaa agccgaggtc tgaaagtttt
    39961 tgctggaagc gagcgatgga atcttccagt gcggcatctt tgccggggat aaatgtttgc
    40021 gtcatgaaaa tcactttagt cgtacggaaa gcgcgcaata atacgggttt tatctcaaag
    40081 gcgctatcac cgccgccatg ccgatgaact gttgactatg cttttagtgg ataaaccacg
    40141 ataagagcat aaaaatgaag gcgttcgatc tccaccgtat ggcatttgat aaagtgcctt
    40201 ttgatttcct tggcgaagtg gcactacgta gtctttatac ctttgtactg gtttttttgt
    40261 tcctcaaaat gaccggaaga cgcggtgtgc ggcagatgtc gctttttgaa gtgttaatca
    40321 ttctgacgct gggatcagcg gcgggagatg tggcgtttta tgatgatgtg ccgatggtcc
    40381 cggtacttat cgtctttatc actctggcgt tgttataccg cctggtaatg tggttgatgg
    40441 cgcacagtga aaaactggaa gatcttctgg aaggcaagcc ggttgtcatt attgaagatg
    40501 gcgagctggc ctggtcaaaa ctcaacaact ccaacatgac ggaatttgag ttctttatgg
    40561 agctacgatt gcgcggtgtg gaacagctgg ggcaggtacg tctggcgatt ctcgaaacca
    40621 acgggcaaat cagtgtttat ttctttgaag atgacaaggt gaaaccgggc ttacttattt
    40681 tacccagtga ctgtactcag cgttacaaag tggtgccgga gtcggcggac tatgcctgca
    40741 tccgttgcag tgaaatcatt catatgaacg cgggggaaaa acaattatgt ccgcgctgtg
    40801 caaatccaga atggacgaag gcaagtcggg caaaacgggt gacgtgacag taaaaacatc
    40861 ggctttttgc taataatccg agagattctt ttgtgtgatg caagccacat ttttgccctc
    40921 aacggtttta ctcattgcga tgtgtgtcac tgaatgataa aaccgatagc cacaggaata
    40981 atgtattacc tgtggtcgca atcgattgac cgcgggttaa tagcaacgca acgtggtgag
    41041 gggaaatggc tcaaatcttc aattttagtt ctggtccggc aatgctaccg gcagaggtgc
    41101 ttaaacaggc tcaacaggaa ctgcgcgact ggaacggtct tggtacgtcg gtgatggaag
    41161 tgagtcaccg gggcaaagag ttcattcagg ttgcagagga agccgagaag gattttcgcg
    41221 atcttcttaa tgtcccctcc aactacaagg tattgttctg ccatggcggc ggtcgcggtc
    41281 agtttgctgc tgttccgttg aatattctcg gtgataaaac cactgcagat tatgttgatg
    41341 ccggttactg ggcggcaagt gccattaaag aagcgaaaaa atactgcacg cctaatgtct
    41401 ttgacgccaa agtgactgtt gatggtctgc gcgcggttaa gccaatgcgc gaatggcaac
    41461 tctctgataa tgctgcttat atgcattatt gcccgaatga aaccatcgac ggtatcgcca
    41521 tcgacgaaac gccagacttc ggcaaagatg tggtggtcgc cgccgacttc tcttcaacca
    41581 ttctttcccg tccgattgac gtcagccgtt atggcgtgat ttacgctggc gcgcagaaaa
    41641 atatcggccc ggcaggcctg acaatcgtca tcgttcgtga agatttgctg ggcaaagcga
    41701 atatcgcgtg tccgtcgatt ctggattatt ccatcctcaa cgataacggc tccatgttta
    41761 acacgccgcc gacatttgcc tggtatctgt ctggtctggt ctttaaatgg ctgaaagcga
    41821 acggcggtgt agctgaaatg gataaaatca atcagcaaaa agcagaactg ctgtatgggg
    41881 tgattgataa cagcga
//
LOCUS       DAMZFT010000027        41011 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.027, whole genome shotgun sequence.
ACCESSION   DAMZFT010000027 DAMZFT010000000
VERSION     DAMZFT010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 41011)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41011)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41011
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.027"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(<1..456)
                     /gene="cheZ"
                     /locus_tag="PN977_001573"
     CDS             complement(<1..456)
                     /gene="cheZ"
                     /locus_tag="PN977_001573"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase CheZ"
                     /protein_id="HDI6041618.1"
                     /translation="MMQPSIKPADEHSAGDIIARIGSLTRMLRDSLRELGLDQAIAEA
                     AEAIPDARDRLYYVVQMTAQAAERALNSVEASQPHQDQMEKSAKALTQRWDDWFADPI
                     DLADARELVTDTRQFLADVPAHTSFTNAQLLEIMMAQDFQDLTGQVIKRM"
     gene            complement(467..856)
                     /gene="cheY"
                     /locus_tag="PN977_001574"
     CDS             complement(467..856)
                     /gene="cheY"
                     /locus_tag="PN977_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002210873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator CheY"
                     /protein_id="HDI6041619.1"
                     /translation="MADKELKFLVVDDFSTMRRIVRNLLKELGFNNVEEAEDGLDALN
                     KLQAGGYGFVISDWNMPNMDGLELLKTIRADGAMSALPVLMVTAEAKKENIIAAAQAG
                     ASGYVVKPFTAATLEEKLNKIFEKLGM"
     gene            complement(871..1920)
                     /gene="cheB"
                     /locus_tag="PN977_001575"
     CDS             complement(871..1920)
                     /gene="cheB"
                     /locus_tag="PN977_001575"
                     /EC_number="3.1.1.61"
                     /EC_number="3.5.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate methylesterase/protein
                     glutamine deamidase"
                     /protein_id="HDI6041620.1"
                     /translation="MSKIRVLSVDDSALMRQIMTEIINSHSDMEMVATAPDPLVARDL
                     IKKFNPDVLTLDVEMPRMDGLDFLEKLMRLRPMPVVMVSSLTGKGSEVTLRALELGAI
                     DFVTKPQLGIREGMLAYSEMIAEKVRTAAKASLAAHKPLSAPTTLKAGPLLSSEKLIA
                     IGASTGGTEAIRHVLQPLPLSSPALLITQHMPPGFTRSFADRLNKLCQIGVKEAEDGE
                     RVLPGHAYIAPGDRHMELARSGANYQIKIHDGPAVNRHRPSVDVLFHSVAKQAGRNAV
                     GVILTGMGNDGAAGMLAMRQAGAWTLAQNEASCVVFGMPREAINMGGVCEVIDLSQVS
                     QQMLAKISAGQAIRI"
     gene            complement(1923..2783)
                     /gene="cheR"
                     /locus_tag="PN977_001576"
     CDS             complement(1923..2783)
                     /gene="cheR"
                     /locus_tag="PN977_001576"
                     /EC_number="2.1.1.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HDI6041621.1"
                     /translation="MTSSLPCGQTSLLLQMTERLALSDAHFRRISQLIYQRAGIVLAD
                     HKRDMVYNRLVRRLRSLGLTDFGHYLNLLESNQHSGEWQAFINSLTTNLTAFFREAHH
                     FPLLADHARRRFGEYRVWSAAASTGEEPYSIAMTLAETLGTVPGRWKVFASDIDTEVL
                     EKARSGIYRHEELKNLTPQQLQRYFMRGTGPHEGLVRVRQELANYVDFAPLNLLAKQY
                     TVPGPFDAIFCRNVMIYFDQTTQQEILRRFVPLLKPDGLLFAGHSENFSHLERRFTLR
                     GQTVYALSKD"
     gene            complement(2802..4403)
                     /gene="tap"
                     /locus_tag="PN977_001577"
     CDS             complement(2802..4403)
                     /gene="tap"
                     /locus_tag="PN977_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein IV"
                     /protein_id="HDI6041622.1"
                     /translation="MFNRIRISTTLFLILILCGILQIGSNGMSFWAFRDDVQRLNQVE
                     QSNQQRAALAQTRAVMLQASTALNKAGTLTALSYPADDIKTLMTTARASLTQSTTLFK
                     SFMAMTAGNEHVRALQKETEKSFARWHNDLEHQATWLESNQLSDFLSAPVQESQNAFD
                     VNFEVWQQEINHVLEGASAQSQRNYHISALVFISMIIVAALYISSALWWTRKMIVQPL
                     AIIGSHFDSIAAGNLARPIAVYGRNEITAIFASLKTMQQALRGTVSDVRKGSQEMHIG
                     IAEIVAGNNDLSSRTEQQAASLAQTAASMEQLTATVGQNADNARQASELAKNAATTAQ
                     AGGVQVSTMTHTMQEIATSSQKIGDIISVIDGIAFQTNILALNAAVEAARAGEQGRGF
                     AVVAGEVRNLASRSAQAAKEIKGLIEESVNRVQQGSKLVNNAAGTMTDIVSSVTRVND
                     IMGEIASASEEQRRGIEQVAQAVSQMDQVTQQNASLVEEAAVATEQLANQADHLSSRV
                     AVFTLEEHEVARHESAQLQIAPVVS"
     gene            complement(4449..6110)
                     /gene="tar"
                     /locus_tag="PN977_001578"
     CDS             complement(4449..6110)
                     /gene="tar"
                     /locus_tag="PN977_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein II"
                     /protein_id="HDI6041623.1"
                     /translation="MINRIRVVTLLVMVLGVFALLQLISGSLFFSSLHHSQKSFVVSN
                     QLREQQGELTSTWDLMLQTRINLSRSAVRMMMDSSNQQSNAKVELLDSARKTLAQAAT
                     HYKKFKSMAPLPEMVATSRNIDEKYKNYHTALTELIDYLDYGNTGAYFAQPTQGMQNA
                     MGEAFAQYALSSEKLYRDIVTDNADDYRFAQWQLAVIALVVILILLVAWYGIRRMLLT
                     PLAKIIAHIREIAGGNLANTLTIDGRSEMGDLAQSVSHMQRSLTDTVTHVREGSDAIY
                     AGTREIAAGNTDLSSRTEQQASALEETAASMEQLTATVKQNADNARQASQLAQSASDT
                     AQHGGKVVDGVVKTMHEIADSSKKIADIISVIDGIAFQTNILALNAAVEAARAGEQGR
                     GFAVVAGEVRNLASRSAQAAKEIKALIEDSVSRVDTGSVLVESAGETMNNIVNAVTRV
                     TDIMGEIASASDEQSRGIDQVALAVSEMDRVTQQNASLVQESAAAAAALEEQASRLTQ
                     AVSAFRLAASPLTNKPQTPSRPASEQPPAQPRLRITEQDPNWETF"
     gene            complement(6255..6758)
                     /gene="cheW"
                     /locus_tag="PN977_001579"
     CDS             complement(6255..6758)
                     /gene="cheW"
                     /locus_tag="PN977_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HDI6041624.1"
                     /translation="MTGMTNVTKLASEPSGQEFLVFTLGDEEYGIDILKVQEIRGYDQ
                     VTRIANTPAFIKGVTNLRGVIVPIVDLRIKFSQVDVDYNDNTVVIVLNLGQRVVGIVV
                     DGVSDVLSLTAEQIRPAPEFAVTLSTEYLTGLGALGDRMLILVNIEKLLNSEEMALLD
                     SAASEVA"
     gene            complement(6779..8743)
                     /gene="cheA"
                     /locus_tag="PN977_001580"
     CDS             complement(6779..8743)
                     /gene="cheA"
                     /locus_tag="PN977_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HDI6041625.1"
                     /translation="MSMDISDFYQTFFDEADELLADMEQHLLVLQPEAPDAEQLNAIF
                     RAAHSIKGGAGTFGFSVLQETTHLMENLLDEARRGEMQLNTDIINLFLETKDIMQEQL
                     DAYKQSQEPDAASFDYICQALRQLALEAKGETPSAVTRLSVVAKSEPQDEQSRSQSPR
                     RIILSRLKAGEVDLLEEELGHLTTLTDVVKGADSLSAILPGDIAEDDITAVLCFVIEA
                     DQITFETVEVSPKISTPPVLKLAAEQAPTGRVEREKTTRSNESTSIRVAVEKVDQLIN
                     LVGELVITQSMLAQRSSELDPVNHGDLITSMGQLQRNARDLQESVMSIRMMPMEYVFS
                     RYPRLVRDLAGKLGKQVELTLVGSSTELDKSLIERIIDPLTHLVRNSLDHGIELPEKR
                     LAAGKNSVGNLILSAEHQGGNICIEVTDDGAGLNRERILAKAASQGLTVSENMSDDEV
                     AMLIFAPGFSTAEQVTDVSGRGVGMDVVKRNIQEMGGHVEIQSKQGTGTTIRILLPLT
                     LAILDGMSVRVADEVFILPLNAVMESLQPREADLHPLAGGERVLEVRGEYLPIVELWK
                     VFNVAGAKTEATQGIVVILQSGGRRYALLVDQLIGQHQVVVKNLESNYRKVPGISAAT
                     ILGDGSVALIVDVSALQAINREQRMANTAA"
     gene            complement(8748..9674)
                     /gene="motB"
                     /locus_tag="PN977_001581"
     CDS             complement(8748..9674)
                     /gene="motB"
                     /locus_tag="PN977_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HDI6041626.1"
                     /translation="MKNQAHPIIVVKRRKAKSHGAAHGSWKIAYADFMTAMMAFFLVM
                     WLISISSPKELIQIAEYFRTPLATAVTGGDRISNSESPIPGGGDDYTQSQGEVNKQPN
                     IEELKKRMEQSRLRKLRGDLDQLIESDPKLRALRPHLKIDLVQEGLRIQIIDSQNRPM
                     FRTGSADVEPYMRDILRAIAPVLNGIPNRISLSGHTDDFPYASGEKGYSNWELSADRA
                     NASRRELMVGGLDSGKVLRVVGMAATMRLSDRGPDDAVNRRISLLVLNKQAEQAILHE
                     NAESQNEPVSALEKPEVAPQVSVPTMPSAEPR"
     gene            complement(9671..10558)
                     /gene="motA"
                     /locus_tag="PN977_001582"
     CDS             complement(9671..10558)
                     /gene="motA"
                     /locus_tag="PN977_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor stator protein MotA"
                     /protein_id="HDI6041627.1"
                     /translation="MLILLGYLVVLGTVFGGYLMTGGSLGALYQPAELVIIAGAGIGS
                     FIVGNNGKAIKGTLKALPLLFRRSKYTKAMYMDLLALLYRLMAKSRQMGMFSLERDIE
                     NPRESEIFASYPRILADSVMLDFIVDYLRLIISGHMNTFEIEALMDEEIETHESEAEV
                     PANSLALVGDSLPAFGIVAAVMGVVHALGSADRPAAELGALIAHAMVGTFLGILLAYG
                     FISPLASVLRQKSAETSKMMQCVKVTLLSNLNGYAPPIAVEFGRKTLYSSERPSFIEL
                     EEHVRAVKNPQQQTTTEEA"
     gene            complement(10685..11263)
                     /gene="flhC"
                     /locus_tag="PN977_001583"
     CDS             complement(10685..11263)
                     /gene="flhC"
                     /locus_tag="PN977_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhC"
                     /protein_id="HDI6041628.1"
                     /translation="MSEKSIVQEARDIQLAMELITLGARLQMLESETQLSRGRLIKLY
                     KELRGSPPPKGMLPFSTDWFMTWEQNVHASMFCNAWQFLLKTGLCNGVDAVIKAYRLY
                     LEQCPQAEEGPLLALTRAWTLVRFVESGLLQLSSCNCCGGNFITHAHQPVGSFACSLC
                     QPPSRAVKRRKLSQNPADIIPQLLDEQRVQAV"
     gene            complement(11266..11616)
                     /gene="flhD"
                     /locus_tag="PN977_001584"
     CDS             complement(11266..11616)
                     /gene="flhD"
                     /locus_tag="PN977_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416406.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhD"
                     /protein_id="HDI6041629.1"
                     /translation="MHTSELLKHIYDINLSYLLLAQRLIVQDKASAMFRLGINEEMAT
                     TLAALTLPQMVKLAETNQLVCHFRFDSHQTITQLTQDSRVDDLQQIHTGIMLSTRLLN
                     DVNQPEEALRKKRA"
     gene            12396..12824
                     /gene="uspC"
                     /locus_tag="PN977_001585"
     CDS             12396..12824
                     /gene="uspC"
                     /locus_tag="PN977_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspC"
                     /protein_id="HDI6041630.1"
                     /translation="MSYSNILVAVAVTPESQQLLAKAVSIARPVKGHISLITLASDPE
                     MYNQLAAPMLEDLRSVMQEETQSFLDKLIQDAGYPVDKTFIAYGELSEHILEVCHKHH
                     FDLVICGNHNHSFFSRASCSAKRVIASSEVDVLLVPLTGD"
     gene            complement(12831..14255)
                     /gene="otsA"
                     /locus_tag="PN977_001586"
     CDS             complement(12831..14255)
                     /gene="otsA"
                     /locus_tag="PN977_001586"
                     /EC_number="2.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalose-phosphate synthase"
                     /protein_id="HDI6041631.1"
                     /translation="MSRLVVVSNRIAPPDEHAASAGGLAVGILGALKAAGGLWFGWSG
                     ETGNEDQPLKKVKKGNITWASFNLSEQDLDEYYNQFSNAVLWPAFHYRLDLVQFQRPA
                     WDGYLRVNALLADKLLPLLQDDDIIWIHDYHLLPFAHELRKRGVNNRIGFFLHIPFPT
                     PEIFNALPTYDTLLEQLCDYDLLGFQTENDRLAFLDCLSNLTRVTTRSAKSHTAWGKA
                     FRTEVYPIGIEPKEIAKQAAGPLPPKLAQLKAELKNVQNIFSVERLDYSKGLPERFLA
                     YEALLEKYPQHHGKIRYTQIAPTSRGDVQAYQDIRHQLENEAGRINGKYGQLGWTPLY
                     YLNQHFDRKLLMKIFRYSDVGLVTPLRDGMNLVAKEYVAAQDPANPGVLVLSQFAGAA
                     NELTSALIVNPYDRDEVAAALDRALTMSLAERISRHAEMLDVIVKNDINHWQECFISD
                     LKQIVPRSAESQQRDKVATFPKLA"
     gene            complement(14230..15030)
                     /gene="otsB"
                     /locus_tag="PN977_001587"
     CDS             complement(14230..15030)
                     /gene="otsB"
                     /locus_tag="PN977_001587"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HDI6041632.1"
                     /translation="MTEPLTETPELSAKYAWFFDLDGTLAEIKPHPDQVVVPDNILQG
                     LQLLATASDGALALISGRSMVELDALAKPYRFPLAGVHGAERRDINGKTHIVHLPDAI
                     ARDISVQLHTVIAQYPGAELEAKGMAFALHYRQAPQHEDALMTLAQRITQIWPQMALQ
                     QGKCVVEIKPRGTSKGEAIAAFMQEAPFIGRTPVFLGDDLTDESGFAVVNRLGGMSVK
                     IGTGATQASWRLAGVPDVWSWLEMITTALQQKRENNRSDDYESFSRSI"
     gene            complement(15197..16183)
                     /gene="araH"
                     /locus_tag="PN977_001588"
     CDS             complement(15197..16183)
                     /gene="araH"
                     /locus_tag="PN977_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026162.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter permease AraH"
                     /protein_id="HDI6041633.1"
                     /translation="MSSVSTSGSGAPKSSFSFGRIWDQYGMLVVFAVLFIACAIFVPN
                     FATFINMKGLGLAISMSGMVACGMLFCLASGDFDLSVASVIACAGVTTAVVINLTESL
                     WIGVAAGLLLGILCGLVNGFVIAKLKINALITTLATMQIVRGLAYIISDGKAVGIEDE
                     SFFALGYANWFGLPAPIWLTVACLIIFGLLLNKTTFGRNTLAIGGNEEAARLAGVPVV
                     RTKIIIFVLSGLVSAIAGIILASRMTSGQPMTSIGYELIVISACVLGGVSLKGGIGKI
                     SYVVAGILILGTVENAMNLLNISPFAQYVVRGLILLAAVIFDRYKQKAKRTV"
     gene            complement(16198..17712)
                     /gene="araG"
                     /locus_tag="PN977_001589"
     CDS             complement(16198..17712)
                     /gene="araG"
                     /locus_tag="PN977_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter ATP binding protein
                     AraG"
                     /protein_id="HDI6041634.1"
                     /translation="MQQSTPYLSFRGIGKTFPGVKALTDISFDCYAGQVHALMGENGA
                     GKSTLLKILSGNYAPTTGSVVINGQEMSFSDTTAALNAGVAIIYQELHLVPEMTVAEN
                     IYLGQLPHKGGIVNRSLLNYEAGLQLKHLGMDIDPDTPLKYLSIGQWQMVEIAKALAR
                     NAKIIAFDEPTSSLSAREIDNLFRVIRELRKEGRVILYVSHRMEEIFALSDAITVFKD
                     GRYVKTFTDMQQVDHDALVQAMVGRDIGDIYGWQPRSYGEERLRLDAVKAPGVRTPIS
                     LAVRSGEIVGLFGLVGAGRSELMKGLFGGTQITAGQVYIDQQPIDIRKPSHAIAAGMM
                     LCPEDRKAEGIIPVHSVRDNINISARRKHVLGGCVINNGWEENNADHHIRSLNIKTPG
                     AEQLIMNLSGGNQQKAILGRWLSEEMKVILLDEPTRGIDVGAKHEIYNVIYALAAQGV
                     AVLFASSDLPEVLGVADRIVVMREGEIAGELLHEQADERQALSLAMPKVSQAVA"
     gene            complement(17782..18771)
                     /gene="araF"
                     /locus_tag="PN977_001590"
     CDS             complement(17782..18771)
                     /gene="araF"
                     /locus_tag="PN977_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter substrate-binding
                     protein AraF"
                     /protein_id="HDI6041635.1"
                     /translation="MHKFTKALAAIGLAAVMSQSAMAENLKLGFLVKQPEEPWFQTEW
                     KFADKAGKDLGFEVIKIAVPDGEKTLNAIDSLAASGAKGFVICTPDPKLGSAIVAKAR
                     GYDMKVIAVDDQFVNAKGKPMDTVPLVMMAATKIGERQGQELYKEMQKRGWDVKESAV
                     MAITANELDTARRRTTGSMDALKAAGFPEKQIYQVPTKSNDIPGAFDAANSMLVQHPE
                     VKHWLIVGMNDSTVLGGVRATEGQGFKAADIIGIGINGVDAVSELSKAQATGFYGSLL
                     PSPDVHGYKSSEMLYNWVAKDVEPPKFTEVTDVVLITRDNFKEELEKKGLGGK"
     gene            19567..20070
                     /locus_tag="PN977_001591"
     CDS             19567..20070
                     /locus_tag="PN977_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin-like protein"
                     /protein_id="HDI6041636.1"
                     /translation="MATAGMLLKLNSQMNREFYASNLYLHLSNWCSEQSLNGTATFLR
                     AQAQSNVTQMMRMFNFMKSVGATPIVKAIDVPGEKLNSLEELFQKTMEEYEQRSSTLA
                     QLADEAKELNDDSTVNFLRDLEKEQQHDGLLLQTILDEVRSAKLAGMCPVQTDQHVLN
                     VVSHQLH"
     gene            complement(20149..20400)
                     /locus_tag="PN977_001592"
     CDS             complement(20149..20400)
                     /locus_tag="PN977_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2766 domain-containing protein"
                     /protein_id="HDI6041637.1"
                     /translation="MSQPLNADQELVSDVVACQLVIKQIQDVLDVIAPVEVREKMSSQ
                     LKNIDFTHHPAAADPVTMRAIQKAIALIELKFTPQGESH"
     gene            complement(20515..20601)
                     /gene="azuC"
                     /locus_tag="PN977_001593"
     CDS             complement(20515..20601)
                     /gene="azuC"
                     /locus_tag="PN977_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein AzuC"
                     /protein_id="HDI6041638.1"
                     /translation="MKLRKILKSMFNNYCKTFKDVPPGNMFR"
     gene            20864..21187
                     /locus_tag="PN977_001594"
     CDS             20864..21187
                     /locus_tag="PN977_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecR-like lipofamily protein"
                     /protein_id="HDI6041639.1"
                     /translation="MRLLILTLSLITLAGCTVTRQAHVGEVDTATGIVRLVYDQAFLQ
                     HAHTDRYVSRGIADRACQQEGYTHAIPFGQPVGNCSLFAGSLCLNTEFTLSYQCHHSA
                     FPVFL"
     gene            21359..21856
                     /gene="ftnA"
                     /locus_tag="PN977_001595"
     CDS             21359..21856
                     /gene="ftnA"
                     /locus_tag="PN977_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin"
                     /protein_id="HDI6041640.1"
                     /translation="MLKPEMIEKLNEQMNLELYSSLLYQQMSAWCSYHTFEGAATFLR
                     RHAQEEMTHMQRLFDYLTDTGNLPRINTVESPFAEYSSLDELFQETYKHEQLITQKIN
                     ELAHAAMTNQDYPTFNFLQWYVSEQHEEEKLFKSIIDKLSLAGKSGEGLYFIDKELST
                     LDTQN"
     gene            complement(21894..22133)
                     /locus_tag="PN977_001596"
     CDS             complement(21894..22133)
                     /locus_tag="PN977_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecH family protein"
                     /protein_id="HDI6041641.1"
                     /translation="MDSIHGHEVLNMMIESGEQYTHASLEAAIKARFGEQARFHTCSA
                     EGMTAGELVAFLAAKGKFIPSEDGFSTDQSKICRH"
     gene            22324..23535
                     /gene="tyrP"
                     /locus_tag="PN977_001597"
     CDS             22324..23535
                     /gene="tyrP"
                     /locus_tag="PN977_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine transporter TyrP"
                     /protein_id="HDI6041642.1"
                     /translation="MKNRTLGSVFIVAGTTIGAGMLAMPLAAAGVGFSVTLILLIGLW
                     ALMCYTALLLLEVYQHVPADTGLGTLAKRYLGRYGQWLTGFSMMFLMYALTAAYISGA
                     GELLASSISDWTGISMSATAGVLLFTFVAGGVVCVGTSLVDLFNRFLFSAKIIFLVVM
                     LVLLLPHIHKVNLLTLPLQQGLALSAIPVIFTSFGFHGSVPSIVSYMDGNIRKLRWVF
                     IIGSAIPLVAYIFWQVATLGSIDSTTFMGLLANHAGLNGLLQALREMVASPHVELAVH
                     LFADLALATSFLGVALGLFDYLADLFQRSNTVGGRLQTGAITFLPPLAFALFYPRGFV
                     MALGYAGVALAVLALIIPSLLTWQSRKHNPQAGYRVKGGRPALVVVFLCGIAVIGVQF
                     LIAAGLLPEVG"
     gene            complement(23597..24262)
                     /gene="yecA"
                     /locus_tag="PN977_001598"
     CDS             complement(23597..24262)
                     /gene="yecA"
                     /locus_tag="PN977_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YecA"
                     /protein_id="HDI6041643.1"
                     /translation="MKTGPLNESELEWLDDILTKYNTDHAILDVAELDGLLTAVLSSP
                     QEIEPEQWLVAVWGGADYVPRWASEKEMTRFMNLAFQHMADTAERLNEFPEQFEPLFG
                     LREVDGSELTIVEEWCFGYMRGVALSDWSTLPDSLKPALEAIALHGTEENFERVEKMS
                     PEAFEESVDAIRLAALDLHAYWMAHPQEKAVQQPIKAEEKPGRNDPCPCGSGKKFKQC
                     CLH"
     gene            complement(24458..24544)
                     /locus_tag="PN977_001599"
     tRNA            complement(24458..24544)
                     /locus_tag="PN977_001599"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(24508..24510),aa:Leu,seq:taa)
     gene            complement(24557..24630)
                     /locus_tag="PN977_001600"
     tRNA            complement(24557..24630)
                     /locus_tag="PN977_001600"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(24596..24598),aa:Cys,seq:gca)
     gene            complement(24685..24760)
                     /locus_tag="PN977_001601"
     tRNA            complement(24685..24760)
                     /locus_tag="PN977_001601"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(24725..24727),aa:Gly,seq:gcc)
     gene            complement(24912..25460)
                     /gene="pgsA"
                     /locus_tag="PN977_001602"
     CDS             complement(24912..25460)
                     /gene="pgsA"
                     /locus_tag="PN977_001602"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HDI6041644.1"
                     /translation="MQFNIPTLLTLFRVILIPFFVLVFYLPVTWSPFAAALIFCVAAV
                     TDWFDGFLARRWNQSTRFGAFLDPVADKVLVAIAMVLVTEHYHSWWVTLPAATMIARE
                     IIISALREWMAELGKRSSVAVSWIGKVKTTAQMVALAWLLWRPNIWVEYAGIALFFVA
                     AVLTLWSMLQYLSAARADLLDQ"
     gene            complement(25517..27349)
                     /gene="uvrC"
                     /locus_tag="PN977_001603"
     CDS             complement(25517..27349)
                     /gene="uvrC"
                     /locus_tag="PN977_001603"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HDI6041645.1"
                     /translation="MSDQFDAKAFLKTVTSQPGVYRMYDAGGTVIYVGKAKDLKKRLS
                     SYFRSNLASRKTEALVAQIQQIDVTVTHTETEALLLEHNYIKLYQPRYNVLLRDDKSY
                     PFIFLSGDTHPRLAMHRGAKHAKGEYFGPFPNGYAVRETLALLQKIFPIRQCENSVYR
                     NRSRPCLQYQIGRCLGPCVEGLVSEEEYAQQVEYVRLFLSGKDDQVLTQLISRMETAS
                     QNLEFEEAARIRDQIQAVRRVTEKQFVSNTGDDLDVIGVAFDAGMACVHVLFIRQGKV
                     LGSRSYFPKVPGGTELSEVVETFVGQFYLQGSQMRTLPGEILLDFNLSDKTLLADSLS
                     ELAGRKINVQTKPRGDRARYLKLARTNAATALTSKLSQQSTVHQRLTALASVLKLPEV
                     KRMECFDISHTMGEQTVASCVVFDANGPLRAEYRRYNITGIRPGDDYAAMNQVLRRRY
                     GKAIDDSKIPDVILIDGGKGQLAQAKNVFAELDVSWDKNHPLLLGVAKGADRKAGLET
                     LFFEPEGEGFSLPPDSPALHVIQHIRDESHDHAIGGHRKKRAKVKNTSSLETIEGVGP
                     KRRQMLLKYMGGLQGLRNASVEEIAKVPGISQGLAEKIFWSLKH"
     gene            complement(27346..28002)
                     /gene="uvrY"
                     /locus_tag="PN977_001604"
     CDS             complement(27346..28002)
                     /gene="uvrY"
                     /locus_tag="PN977_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UvrY/SirA/GacA family response regulator
                     transcription factor"
                     /protein_id="HDI6041646.1"
                     /translation="MINVLLVDDHELVRAGIRRILEDIKGIKVVGEASCGEDAVKWCR
                     TNAVDVVLMDMSMPGIGGLEATRKIARSTADVKIIMLTVHTENPLPAKVMQAGAAGYL
                     SKGAAPQEVVSAIRSVYSGQRYIASDIAQQMALSQIEPEKTESPFASLSERELQIMLM
                     ITKGQKVNEISEQLNLSPKTVNSYRYRMFSKLNIHGDVELTHLAIRHGLCNAETLSSQ
                     "
     gene            28298..28474
                     /locus_tag="PN977_001605"
     CDS             28298..28474
                     /locus_tag="PN977_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6041647.1"
                     /translation="MIKIFIGHYINVFYSTADITLKKQPLLFLAKLMVYSAALTFFTA
                     NFHCNMTRKINEYA"
     gene            28461..28685
                     /locus_tag="PN977_001606"
     CDS             28461..28685
                     /locus_tag="PN977_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2594 family protein"
                     /protein_id="HDI6041648.1"
                     /translation="MSTPDFSTAENNQELANEVSCLKAMLTLMLQAMGQADAGRVMLK
                     MEKQLALIEDETQAAVFSKTVKQIKQAYRQ"
     gene            complement(28752..29474)
                     /gene="sdiA"
                     /locus_tag="PN977_001607"
     CDS             complement(28752..29474)
                     /gene="sdiA"
                     /locus_tag="PN977_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SdiA"
                     /protein_id="HDI6041649.1"
                     /translation="MQDTDFFSWRRTMLLRFQRMEAAEEVYHEIELQAQQLEYDYYSL
                     CVRHPVPFTRPKVAFYTNYPESWVSYYQAKNFLAIDPVLNPENFSQGHLMWNDDLFSE
                     AQPLWEAARAHGLRRGVTQYLMLPNRALGFLSFSRCSAREIPILSDELQLKMQLLVRE
                     SLMALMRLNDEIVMTPEMNFSKREKEILKWTAEGKTSAEIAMILSISENTVNFHQKNM
                     QKKINAPNKTQVACYAAATGLI"
     gene            complement(29704..30456)
                     /gene="yecC"
                     /locus_tag="PN977_001608"
     CDS             complement(29704..30456)
                     /gene="yecC"
                     /locus_tag="PN977_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine ABC transporter ATP-binding protein
                     YecC"
                     /protein_id="HDI6041650.1"
                     /translation="MSAIEVKNLVKKFHGQTVLHGIDLEVKPGEVVAIIGPSGSGKTT
                     LLRSINLLEQPEAGTITVGDITIDTARSLSQQKSLIRQLRQHVGFVFQNFNLFPHRTV
                     LENIIEGPVIVKGEPKEEATERARELLAKVGLAGKETSYPRRLSGGQQQRVAIARALA
                     MRPEVILFDEPTSALDPELVGEVLNTIRQLAQEKRTMVIVTHEMSFARDVADRAIFMD
                     QGRIVEQGAAKALFADPKQPRTRQFLEKFLLQ"
     gene            complement(30453..31121)
                     /gene="tcyL"
                     /locus_tag="PN977_001609"
     CDS             complement(30453..31121)
                     /gene="tcyL"
                     /locus_tag="PN977_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter permease"
                     /protein_id="HDI6041651.1"
                     /translation="MQESIQLVIDSLPFLLKGAGYTLQLSIGGMFFGLLLGFILALMR
                     LSPIWPVRWLARFYISIFRGTPLIAQLFMIYYGLPQFGIELDPIPSAMIGLSLNTAAY
                     AAETLRAAISSIDKGQWEAAASIGMTPWQTMRRAILPQAARVALPPLSNSFISLVKDT
                     SLAATIQVPELFRQAQLITSRTLEVFTMYLAASLIYWIMATVLSTLQNHFENQLNRQE
                     REPK"
     gene            complement(31136..32122)
                     /gene="dcyD"
                     /locus_tag="PN977_001610"
     CDS             complement(31136..32122)
                     /gene="dcyD"
                     /locus_tag="PN977_001610"
                     /EC_number="4.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-cysteine desulfhydrase"
                     /protein_id="HDI6041652.1"
                     /translation="MPLHNLTRFPRLEFIGAPTPLEYLPRFSDYLGREIFIKRDDVTP
                     MAMGGNKLRKLEFLAADALREGADTLITAGAIQSNHVRQTAAVAAKLGLHCVALLENP
                     IGTTAENYLTNGNRLLLDLFNTQIEMCDALTDPNAQLEELATRVEAQGFRPYVIPVGG
                     SNALGALGYVESALEIAQQCEGAVNISSVVVASGSAGTHAGLAVGLEHLMPESELIGV
                     TVSRSVADQLPKVVNLQQAIAKELELTASAEILLWDDYFAPGYGVPNDEGMEAVKLLA
                     RLEGILLDPVYTGKAMAGLIDGISQKRFKDEGPILFIHTGGAPALFAYHPHV"
     gene            complement(32227..33027)
                     /gene="tcyJ"
                     /locus_tag="PN977_001611"
     CDS             complement(32227..33027)
                     /gene="tcyJ"
                     /locus_tag="PN977_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter substrate-binding
                     protein"
                     /protein_id="HDI6041653.1"
                     /translation="MKLAHLGRQALMGVMAVALGAGMSVKSFADEGLLNKVKERGTLL
                     VGLEGTYPPFSFQGDDGKLTGFEVEFAQQLAKHLGVEASLKPTKWDGMLASLDSKRID
                     VVINQVTISDERKKKYDFSTPYTISGIQALVKKGNEGTLKTAADLKGKKVGVGLGTNY
                     EEWLRQNVQGVDVRTYDDDPTKYQDLRVGRIDAILVDRLAALDLVKKTNDTLAVTGEA
                     FSRQESGVALRKGNEDLLKAVNDAIAEMQKDGTLQALSEKWFGADVTK"
     gene            complement(33115..33666)
                     /gene="fliZ"
                     /locus_tag="PN977_001612"
     CDS             complement(33115..33666)
                     /gene="fliZ"
                     /locus_tag="PN977_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310687.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliZ"
                     /protein_id="HDI6041654.1"
                     /translation="MMVQHLKRRPLSRYLKDFKHSQTHCAHCRKLLDRITLVRDGKIV
                     NKIEISRLDTLLDENGWQVEQQSWAALCRFCGDLHCKTQSDFFDIIGFKQFLFEQTEM
                     SPGTVREYVVRLRRLGNHLHEQNISLDQLQDGFLDEILAPWLPTTSTNNYRIALRKYQ
                     HYQRQTCTGLVQKSSSLPASDIY"
     gene            complement(33712..34431)
                     /gene="fliA"
                     /locus_tag="PN977_001613"
     CDS             complement(33712..34431)
                     /gene="fliA"
                     /locus_tag="PN977_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor FliA"
                     /protein_id="HDI6041655.1"
                     /translation="MNSLYTAEGVMDKHSLWQRYVPLVRHEALRLQVRLPASVELDDL
                     LQAGGIGLLNAVERYDALQGTAFTTYAVQRIRGAMLDELRSRDWVPRSVRRNAREVAQ
                     AIGQLEQELGRNATETEVAERLGIDIADYRQMLLDTNNSQLFSYDEWREEHGDSIELV
                     TDDHQRENPLQQLLDSNLRQRVMEAIETLPEREKLVLTLYYQEELNLKEIGAVLEVGE
                     SRVSQLHSQAIKRLRTKLGKL"
     gene            complement(34751..36388)
                     /locus_tag="PN977_001614"
     CDS             complement(34751..36388)
                     /locus_tag="PN977_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000079690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin FliC"
                     /protein_id="HDI6041656.1"
                     /translation="MAQVINTNSLSLITQNNINKNQSALSSSIERLSSGLRINSAKDD
                     AAGQAIANRFTSNIKGLTQAARNANDGISVAQTTEGALSEINNNLQRIRELTVQASTG
                     TNSDSDLDSIQDEIKSRLDEIDRVSGQTQFNGVNVLAKDGSMKIQVGANDGQTITIDL
                     KKIDSDTLGLSGFNVNGSADKASVAATADGMVKDGYIKGLTSSDGSTAYTKTTANTAA
                     KGSDILAALKTGDKITATGANSLADNATSTTYTYNATSNTFSYTADGVNQTNAAANLI
                     PAAGKTTAASVTIGGTAQNVNIDDSGNITSSDGDQLYLDSTGNLTKNQAGNPNKATVS
                     GLLGNTDAKGTAVKTTIKTEAGVTVTAEGNTGTVKIEGATVSASAFTGIAYSANTGGN
                     TYAVAANNTTNGFLAGDALTQDAQTVSTYYSQADGTVTNSAGKEIYKDADGVYSTENK
                     TSKTSDPLAALDDAISSIDKFRSSLGAIQNRLDSAVTNLNNTTTNLSEAQSRIQDADY
                     ATEVSNMSKAQIIQQAGNSVLAKANQVPQQVLSLLQG"
     gene            36654..38060
                     /gene="fliD"
                     /locus_tag="PN977_001615"
     CDS             36654..38060
                     /gene="fliD"
                     /locus_tag="PN977_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HDI6041657.1"
                     /translation="MASISSLGVGSGLDLSSILDSLTAAQKATLTPISNQQSSFTAKL
                     SAYGTLKSALTTFQTANTALSKADLFSATSTTSSTTAFSATTAGNAIAGKYTISVTHL
                     AQAQTLTTRTTRDDTKTAIATSDSKLTIQQGGDKDPITIDISAANSSLSGIRDAINNA
                     KAGVSASIINVGNGEYRLSVTSNDTGLDNAMTLSVSGDDALQSFMGYDASASSNGMEV
                     SVAAQNAQLTVNNVAIENSSNTISDALENITLNLNDVTTGNQTLTITQDTSKAQTAIK
                     DWVNAYNSLIDTFSSLTKYTAVDAGADSQSSSNGALLGDSTLRTIQTQLKSMLSNTVS
                     SSNYKTLAQIGITTDPSDGKLELDADKLTAALKKDASGVGALIVGDGKKTGITTTIGS
                     NLTSWLSTTGIIKAATDGVSKTLNKLTKDYNAASDRIDAQVARYKEQFTQLDVLMTSL
                     NSTSSYLTQQFENNSNSK"
     gene            38085..38495
                     /gene="fliS"
                     /locus_tag="PN977_001616"
     CDS             38085..38495
                     /gene="fliS"
                     /locus_tag="PN977_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HDI6041658.1"
                     /translation="MYAAKGTQAYAQIGVESAVMSASQQQLVTMLFDGVLSALVRARL
                     FMQDNNQQGKGVSLSKAINIIENGLRVSLDEESKDELTQNLIALYSYMVRRLLQANLR
                     NDVSAVEEVETLMRNIADAWKESLLSPSLIQDPV"
     gene            38495..38860
                     /gene="fliT"
                     /locus_tag="PN977_001617"
     CDS             38495..38860
                     /gene="fliT"
                     /locus_tag="PN977_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001015018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliT"
                     /protein_id="HDI6041659.1"
                     /translation="MNHAPHLYFAWQQLVEKSQLMLRLATEEQWDELIASEMAYVNAV
                     QEIAHLTEEIDPSTTMQEQLRPMLRLILDNESKVKQLLQIRMDELAKLVGQSSVQKSV
                     LSAYGDQGGFVLAPQDNLS"
     gene            38938..40425
                     /gene="amyA"
                     /locus_tag="PN977_001618"
     CDS             38938..40425
                     /gene="amyA"
                     /locus_tag="PN977_001618"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HDI6041660.1"
                     /translation="MRNPTLLQCFHWYYPEGGKLWPELAERADGFNDIGINMVWLPPA
                     YKGASGGYSVGYDSYDLFDLGEFDQKGSIPTKYGDKAQLLAAIDALKRNDIAVLLDVV
                     VNHKMGADEKEAIRVQRVNADDRTQIDEEIIECEGWTRYTFPARAGQYSQFIWDFKCF
                     SGIDHIENPDEDGIFKIVNDYTGEGWNDQVDDELGNFDYLMGENIDFRNHAVTEEIKY
                     WARWVMEQTQCDGFRLDAVKHIPAWFYKEWIEHVQEVAPKPLFIVAEYWSHEVDKLQT
                     YIDQVEGKTMLFDAPLQMKFHEASRMGRDYDMTQIFTGTLVEADPFHAVTLVANHDTQ
                     PLQALEAPVEPWFKPLAYALILLRENGVPSVFYPDLYGAHYEDVGGDGQTYPIDMPII
                     EQLDELILARQRFAHGVQTLFFDHPNCIAFSRSGTDEYPGCVVVMSNGDDGEKTIHLG
                     ENYGNKTWRDFLGNRQESVVTDENGEATFFCNGGSVSVWVIEEVI"
     gene            complement(40459..40872)
                     /locus_tag="PN977_001619"
     CDS             complement(40459..40872)
                     /locus_tag="PN977_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310694.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HDI6041661.1"
                     /translation="MKKLAIAGALMLLAGCAEVENYNNVVKTPAPDWLAGYWQTKGPQ
                     RALVSPEAIGSLIVIKEGDTLDCRQWQRVIAVPGKLTLMSDDLTNVTVKRELYEVERD
                     GNTIEYDGMTMERVDRPTAECAAALDKAPLPTPLP"
BASE COUNT        10091 a        10733 c         9728 g        10459 t
ORIGIN      
        1 catccgctta atgacctgcc cggtgagatc ctgaaaatcc tgcgccatca tgatttccag
       61 cagttgcgcg ttagtaaagc tggtatgcgc ggggacatct gccagaaatt gccgcgtatc
      121 tgttaccagt tcacgggcgt cggcaaggtc aatcggatcg gcaaaccagt catcccaacg
      181 ttgggttaac gcttttgctg atttctccat ttgatcctga tgcggttgtg acgcctcaac
      241 actgttcagc gcccgctccg cagcctgggc ggtcatctgc acaacatagt acaaacgatc
      301 gcgcgcatcg gggatggctt ccgccgcttc ggcaatggcc tgatccagcc ccagctcccg
      361 caaactgtcg cgcagcatac gcgtcaggct gccgatgcgc gcaatgatat cgccagctga
      421 atgctcgtca gcaggtttga ttgatggttg catcatagtc gcatcctcac atgcccagtt
      481 tctcaaagat tttgttgagt ttttcctcca gcgtcgcggc ggtaaatggc ttcaccacat
      541 agccactggc ccccgcttgc gccgcagcaa tgatgttctc tttcttcgct tctgcagtca
      601 ccattaacac tggcaatgcc gacatcgcgc catccgcacg aatcgttttc agcaattcca
      661 ggccatccat attgggcatg ttccagtcgg agataacaaa tccataaccg cctgcctgca
      721 acttattgag cgcgtcgagg ccatcttccg cttcctcaac attattgaat cccagctctt
      781 tcagcaggtt acgcactatg cgtcgcatgg tggaaaagtc atccacaacc aaaaatttaa
      841 gttctttatc cgccatttca cactcctgat ttaaatacgt atcgcctgtc cggcactaat
      901 ttttgccaac atttgctggc ttacctggct aagatcgatc acttcgcaga caccacccat
      961 attgatggcc tcgcgcggca tgccgaacac cacgcaactt gcttcgtttt gcgcaagggt
     1021 ccatgccccc gcctgacgca tcgccaacat tcccgccgca ccgtcgttgc ccataccggt
     1081 caggatcacc ccaaccgcat tacgccccgc ctgtttggcg acagaatgga acaacacatc
     1141 taccgaaggc cgatgacggt taaccgccgg gccatcgtga attttgattt ggtaatttgc
     1201 gccactacgc gccagctcca tatgccgatc gcccggcgca atataggcat gccccggtaa
     1261 gacacgttct ccgtcttcgg cttctttaac cccgatctgg caaagcttat taagtctgtc
     1321 ggcaaaagag cgggtgaaac cgggcggcat atgctgggta attaacagtg ccgggctgga
     1381 aagcggcaac ggttgcagca cgtgacgaat tgcctcagtt ccccccgttg aagcaccaat
     1441 cgcaatcagt ttttcagaac tcaacaacgg ccctgccttc agcgttgtcg gtgccgacaa
     1501 tggcttatgt gctgcaaggc tcgcctttgc tgctgtacgc accttttcag caatcatttc
     1561 gctatacgcc agcattcctt cgcgaatacc cagttgcggt ttggtgacaa aatctatcgc
     1621 ccccagctcc agcgcgcgca gcgtgacttc tgaccctttg ccggtcaggg aagaaaccat
     1681 cacaacgggc attggacgca aacgcattaa tttttcgaga aaatccagtc cgtccatccg
     1741 cggcatttca acatccagcg tcaatacatc gggattgaat tttttaatca aatcacgcgc
     1801 aaccagcgga tcaggggcgg tcgccaccat ttccatgtcg ctatggctgt tgatgatttc
     1861 tgtcatgatc tggcgcatca gtgccgaatc atcgacagat aacaccctga ttttgctcat
     1921 cgttaatcct tacttagcgc atacaccgtc tgaccacgca gcgtgaagcg gcgctcaagg
     1981 tggctaaagt tttcagagtg acccgcaaac agcaatccgt cgggtttaag gagcggaaca
     2041 aagcggcgca aaatctcctg ctgggtagtt tgatcgaagt agatcatgac gttacgacag
     2101 aagatcgcat caaacggccc cggcacggtg tactgtttcg ccagtaaatt cagcggggca
     2161 aaatcaacat agttcgccag ctcctgacgc acgcgtacca gcccttcatg cggtcccgtc
     2221 cctcgcatga aataccgttg cagttgctgc ggcgtcaggt ttttcaactc ttcatggcga
     2281 taaataccgc ttctggcttt ttccagcact tcggtgtcga tatcgctggc aaacactttc
     2341 cagcgtccgg gcacggtgcc caatgtttca gccagcgtca tcgcaatgct gtacggctct
     2401 tcgccggtcg aagccgccgc gctccatacg cgatactcgc caaaacggcg acgtgcgtga
     2461 tccgcgagca gagggaaatg gtgtgcctca cggaaaaatg ccgtcagatt cgtggtcagc
     2521 gaattgataa acgcctgcca ctcaccgctg tgctgattag attccagcaa gttcaggtaa
     2581 tggccgaaat ccgtcagtcc cagcgaacgc aaacgacgaa ccagtcggtt gtaaaccatg
     2641 tcgcgtttat ggtcggccag gacgatcccg gctcgttgat agatcaattg acttatccgc
     2701 cgaaaatgcg cgtcggaaag cgccaggcgc tcggtcatct gtaacaataa agacgtttgc
     2761 ccacagggca gagatgaagt catagcgcct tctcaatcac ttcaggatac cactggcgca
     2821 atttgtaact gcgccgactc atgtcgtgct acttcatgct cttcaagggt aaataccgcc
     2881 acgcgcgacg aaagatggtc ggcctgattc gccagttgtt ccgttgccac cgccgcttct
     2941 tctaccaggg aggcgttctg ctgtgtcacc tgatccatct ggctgacagc ctgtgcaacc
     3001 tgctcaatcc cccgccgttg ttcttccgag gccgaagcaa tttctcccat aatgtcgttc
     3061 acgcgggtca ccgaactgac aatatcggtc atggtcccgg cggcgttatt caccagtttc
     3121 gaaccctgct ggacacgatt gactgactct tcgatcagcc ctttgatctc ttttgctgcc
     3181 tgggcgctac ggctggcaag attgcgcact tcacctgcca cgaccgcaaa accacgcccc
     3241 tgctctccgg cgcgagccgc ttccactgcc gcattcaggg ccagaatatt ggtctggaaa
     3301 gcaatgccgt cgataacgct gataatgtcg ccaatttttt gcgaactggt ggcgatctcc
     3361 tgcatggtgt gagtcatggt actgacctga acaccgcccg cctgcgccgt tgtcgcggca
     3421 ttttttgcca gttccgacgc ctgtcgtgcg ttatcggcgt tttgccctac cgtggcggtt
     3481 aattgctcca tactggcggc cgtttgtgcc agcgatgccg cctgctgttc ggtgcgactt
     3541 gagagatcgt tattgcctgc gacaatctcc gcgataccaa tgtgcatctc ctggcttccc
     3601 ttacgcacat cacttaccgt cccacgcaaa gcctgctgca tggtcttcag actggcaaaa
     3661 atggcggtga tctcattgcg accatatacc gcaatcggac gcgccagatt acccgcagca
     3721 atgctgtcaa aatggctacc gatgatggcc agtggttgaa caatcatctt gcgcgtccac
     3781 cacagcgcac tgctgatgta gagcgctgca acaataatca tgctgataaa caccagcgcc
     3841 gaaatgtgat agttacgctg gctttgcgca ctggcacctt ccagcacatg gttgatctcc
     3901 tgctgccaga cctcaaagtt aacgtcaaac gcattttgtg attcctgcac cggcgctgag
     3961 aggaaatccg aaagttgatt actttcaagc caggtcgcct gatgttcgag atcgttgtgc
     4021 cagcgggcaa aacttttctc cgtctctttt tgcaacgccc tgacgtgctc gttgcccgca
     4081 gtcatcgcca taaaactttt aaacagcgtg gtggattgcg tcagactggc gcgcgccgtc
     4141 gtcatcaacg ttttaatgtc atccgccgga tagctaagcg ccgtcagagt gcccgctttg
     4201 ttcagcgcgg tactggcctg taacattacc gcccgagttt gcgctaaagc cgcacgttgc
     4261 tgattgctct gctcgacctg attcagtcgt tgcacatcgt cgcgaaatgc ccaaaaagac
     4321 atgccgttac tgccaatctg caagatcccg cagagaatca aaattaaaaa cagcgtggtc
     4381 gaaattcgaa tacgattaaa catcaacgct ccccatcagg cggcaatgac cgcgttagta
     4441 aatactcgtc aaaatgtttc ccagtttgga tcttgttcag taattcgcag tcgtggctgt
     4501 gccggtggtt gctcactggc aggacgggat ggtgtttgcg gtttattggt gagtgggctg
     4561 gctgccagac ggaacgcgga aaccgcttgc gttaaacgac tcgcctgttc ttccagcgca
     4621 gctgcggcgg cagctgattc ctgcaccagc gatgcgttct gttgcgtgac gcgatccatt
     4681 tccgaaaccg ccaatgcgac ttgatcgatg ccacggctct gttcatccga tgccgatgca
     4741 atctcgccca taatgtcagt cacgcgagtg acagcattga cgatattgtt cattgtttcc
     4801 ccggcgcttt cgaccagcac cgaaccggta tcaacgcgcg agacggagtc ttcaatgagg
     4861 gctttgatct cttttgccgc ctgggcgctg cgactggcaa gattacgcac ttcgcccgcc
     4921 accacggcaa aaccacggcc ctgttcaccc gctcgcgcgg cttcaacagc ggcattcagc
     4981 gcgagtatat tagtctggaa ggcaataccg tcgataacgc tgataatgtc ggcaattttc
     5041 ttcgaactat cggcgatctc atgcatcgtt ttcactacgc catccaccac tttgccgccg
     5101 tgctgggcgg tgtcggaggc actttgcgcc agttgcgagg cctggcgggc gttatcggcg
     5161 ttttgcttca ctgtcgcggt gagctgctcc atgctggcgg cagtttcttc cagcgcggat
     5221 gcctgctgtt ccgtacggga ggaaagatcg gtgttgcccg ccgcaatttc acgggtaccg
     5281 gcatagatgg catctgaacc ttcacgcaca tgagtgacgg tgtcagtcaa agagcgttgc
     5341 atatgtgaaa cgctctgcgc caggtcgccc atttcactgc gcccgtcaat ggtcagggta
     5401 ttcgccaggt taccaccggc gatttcgcga atgtgagcaa taatttttgc cagcggtgta
     5461 agcaacatac ggcgaatgcc gtaccacgcc accagcagaa tcaatatcac caccagcgcg
     5521 ataaccgcca gttgccactg ggcaaatcgg taatcatctg cgttgtcagt gacgatatcg
     5581 cgatacagtt tttcactgct gagggcgtac tgagcaaacg cttcgcccat tgcattttgc
     5641 attccctggg ttggctgagc gaaataagct ccagtattgc cataatcaag ataatcaatc
     5701 agttcagtta acgctgtgtg atagttttta tatttttcat caatattacg actggtagcg
     5761 accatttcag gtaacggtgc catgcttttg aattttttat aatgcgtcgc ggcctgggcc
     5821 aatgttttcc tggcgctatc gagcaattca actttggcat tactttgctg attggatgaa
     5881 tccatcatca tccgcaccgc tgaacgactc aggttaatgc gcgtttgcag cattaaatcc
     5941 caggttgacg tcagctcgcc ctgctgttcc cgtaattgat tggaaaccac aaagctcttc
     6001 tggctatggt gaagggaaga aaaaaacaga ctgccggaaa taagctgtaa cagtgcaaat
     6061 acccccagca ccattaccaa cagcgtgact acgcggatac ggttaatcat aaggcacctt
     6121 cctgaaaaca agttgatctc gttatcggca aggaaggggg aaactttatt gctgatgcca
     6181 cccgccgcga aattgaaata aaaaacccga tgcgcagatc atcgggttca tttcaattga
     6241 ggaaatcggg agaattacgc cacttctgac gccgcgctat ctaacagcgc catctcttcg
     6301 ctgttcagca gtttttcgat gttcaccaga atcaacatcc ggtcgcccag tgcgcccagt
     6361 cctgtgagat attcggttga aagcgtcacg gcaaattccg gtgccggacg aatttgctcc
     6421 gccgtcaatg aaagcacgtc tgagacgccg tcaaccacga tgccgaccac ccgctgtccg
     6481 agattcagga cgataactac cgtgttgtcg ttatagtcca catccacctg gctgaactta
     6541 attcgtaagt caacaatcgg cacaataacg ccgcgcagat tcgtgacgcc tttgataaac
     6601 gctggcgtgt tcgcaatccg tgtcacctga tcgtagccac ggatctcctg cactttcaga
     6661 atatcaatac cgtactcttc atcaccaagg gtaaatacca gaaattcctg gcctgacggc
     6721 tcgctggcca gctttgttac attcgtcata ccggtcatat tgttaccttt ttactcattc
     6781 aggcggcggt gttcgccata cgttgttcgc ggtttatcgc ctgcaaggcg gagacatcaa
     6841 caatcagtgc cacgctgccg tcgccaagaa tggtcgcagc agaaatgccg gggactttgc
     6901 gatagttact ttccaggttt ttaaccacaa cctggtgttg accaattaat tgatccacca
     6961 gcaaggcgta gcggcgaccg ccactttgta agatcaccac aattccctgg gtagcttcgg
     7021 ttttcgcgcc cgcgacgttg aacactttcc acagttcgac gatgggcaga tattcacccc
     7081 gcacttccag cacccgctcg ccgcctgcca gtggatgcag atcggcttca cggggttgca
     7141 gtgattccat gacagcattc agcggcagaa tgaaaacttc atccgcaacg cgtacggaca
     7201 tgccgtcgag gatggccagc gtcagcggca gtaaaatgcg gatcgtcgtg ccagtaccct
     7261 gcttcgactg gatttcgaca tgaccgccca tctcctggat attacgttta acgacgtcca
     7321 tgccgacgcc gcgcccggag acgtcggtga cctgctctgc cgtggagaag ccaggtgcaa
     7381 atatcagcat cgcgacttcg tcgtcgctca tgttttcgct tacagtcaaa ccttgcgagg
     7441 ccgcttttgc cagaattcgc tcacggttta gccctgcacc atcgtcggtc acttcaatgc
     7501 aaatgttgcc gccctgatgt tcggcagaaa gaattaaatt tccgacgctg tttttacctg
     7561 cggcgagccg tttttctggc agttcaatac cgtgatcgag gctattgcgt accaggtggg
     7621 tcagcgggtc gataatgcgt tctatcaggc tcttgtcgag ttcagtagaa ctgcccacca
     7681 gcgtcagttc tacctgcttg ccgagttttc ccgccagatc acgcaccagc cggggatagc
     7741 gactaaagac atattccatc ggcatcatac gaatcgacat cactgattct tgcaaatcac
     7801 gagcgttacg ttgtaactgc cccatgctgg taatcaaatc accatgatta accgggtcca
     7861 gttcactgga gcgctgggca agcatggact gggtgatgac cagctcgccg acgaggttaa
     7921 ttaattgatc aaccttttct accgctacac ggatgctggt ggattcattg ctgcgcgtcg
     7981 ttttttcccg ctccacgcgg ccggttggcg cttgttcggc tgccagttta agcactggtg
     8041 gggtggatat ttttggcgag acttctactg tttcaaaagt aatctgatcg gcttcaatca
     8101 caaaacagag taccgctgtg atgtcatctt cggcaatgtc gcccggtaat attgccgaga
     8161 gcgaatccgc ccctttcacc acgtcagtta acgttgtcag atgtcccagt tcttcttcca
     8221 gcaggtcgac ttccccggcc ttcaggcgcg aaaggataat tcgtcgcggc gactgactgc
     8281 gactctgctc atcttgcggt tcacttttgg caaccacact taatcgggtc actgcggatg
     8341 gcgtttcgcc tttcgcttct aatgccagtt gacgcaaggc ctggcagata taatcgaagc
     8401 tggcggcatc cggctcttgc gactgtttat aagcgtcgag ctgttcttgc atgatgtcct
     8461 tcgtttccaa aaacagattg ataatgtcgg tgttgagttg catctcacct cgtctggctt
     8521 catcgagcag gttttccatc agatgcgtgg tttcctgcaa aacgctgaag ccaaaagttc
     8581 ctgcccctcc tttgatcgag tgggcagccc gaaagatggc attcaattgt tcggcatctg
     8641 gcgcttccgg ctgcaaaacc agcagatgct gctccatgtc agccaacagt tcgtccgctt
     8701 catcaaaaaa tgtctgataa aaatcgctta tatccatgct cacgctgtca cctcggttcg
     8761 gctgatggca ttgtgggaac actgacctgt ggtgcaacct caggtttttc cagggcgctt
     8821 actggctcat tctggctttc ggcgttttca tgcaaaatgg cctgttcggc ttgtttgttc
     8881 agtaccagca ggctgatgcg acggttgacg gcatcatcag gtccgcgatc gcttaagcgc
     8941 atcgttgccg ccatgccgac aacacgtaac actttgccgc tatccaaccc gccgaccatc
     9001 agttcgcggc gggatgcatt ggcccgatcg gcagaaagct cccagttgct gtaacctttc
     9061 tcaccgctgg cgtagggaaa atcatcggta tgacctgaaa ggctaatacg gttgggaata
     9121 ccgttcagta caggcgcgat ggcgcgcaga atgtcgcgca tatagggttc gacatcggca
     9181 ctgccggttc taaacatcgg gcgattctgg ctatcgatga tctgaatacg tagaccttcc
     9241 tggaccagat cgattttgag atgtggacgc aacgcccgca gtttcggatc ggactctatc
     9301 aactggtcga gatcaccccg caatttccgc aatcgacttt gctccatgcg ttttttcagc
     9361 tcttcgatgt tcggctgctt attcacttcc ccctggcttt gggtgtaatc atcaccaccg
     9421 ccgggaattg ggctttcact attagaaatg cgatcgccgc ccgtaaccgc agtcgccagt
     9481 ggagtccgga agtactccgc aatctgaatc agctcttttg ggctggagat ggagatcagc
     9541 cacatcacca gaaaaaaggc catcatcgca gtcataaagt cggcataagc aatcttccac
     9601 gatccatgtg ctgccccgtg gcttttggct ttgcgtcgtt tgacgacaat aatcggatgc
     9661 gcttgattct tcatgcttcc tcggttgtcg tctgttgttg cggatttttc accgcacgca
     9721 catgctcttc cagttcaata aacgacggac gttcgctgga atagagcgtt ttgcgaccaa
     9781 actcaacggc gataggcggt gcgtaaccgt tcagattaga aagcagagtg actttgacgc
     9841 actgcatcat tttgctggtt tcggcgcttt tctgacgtaa aacactcgct aatggggaaa
     9901 taaatccgta agccaataaa atgccgagga aagtccccac catcgcatgt gcgataagcg
     9961 cacccagctc ggcggcagga cgatcggctg aacctaacgc gtgaacgacc cccattacag
    10021 ccgcgacaat accaaacgcc ggaagtgagt ccccgaccag cgccagactg ttcgccggga
    10081 cttctgcctc gctttcgtgc gtctcaatct cttcatccat cagggcttcg atttcgaagg
    10141 tgttcatatg accgctgata atcagacgca gataatcgac gataaaatca agcatgacgc
    10201 tatccgcgag gatgcgtggg tagctggcga agatctcgct ctcacgggga ttttcaatat
    10261 cacgttccag cgaaaacatc cccatctgcc gcgatttcgc catcaaccga taaagcagag
    10321 ccagcagatc catatacatt gctttggtgt atttggagcg acgaaacagc aacggcagcg
    10381 ccttcagcgt gcctttaatc gctttgccat tattgccgac gataaacgac ccaatccctg
    10441 caccggcaat aatcaccagt tcagcgggtt gatagagtgc tccaaggctt ccaccggtca
    10501 tcaaataacc gccgaaaact gtaccgagaa caaccaggta acctaataag ataagcacga
    10561 catcatcctt ccactgttga ccatgacagg atgttcagtc gtcaggcgtt aacgcgcgat
    10621 tggggcaaaa aaaagcagcg gtacgtcgtt accgctgctg gaatgttgcg cctcaccgta
    10681 tcagttaaac agcctgtact ctctgttcat ccagcagttg tgggataata tcggcaggat
    10741 tctgggaaag tttacgtctt tttactgccc gggatggcgg ttgacataag ctgcaggcaa
    10801 agctgccaac aggctggtga gcgtgggtaa taaaattgcc gccgcagcag ttgcagctgg
    10861 aaagttgcag taatccactt tcaacaaacc gcaccaatgt ccaggcacgg gttaatgcca
    10921 gcagtggtcc ttcttctgct tgtgggcact gttcaaggta taaacggtag gctttgatca
    10981 ccgcatcgac gccattacac aaaccggttt tcagtaaaaa ctgccatgca ttacagaaca
    11041 tcgaagcatg aacgttttgt tcccaggtca taaaccagtc ggttgagaat ggcagcatgc
    11101 ctttcggcgg tgggcttccg cgcagttctt tataaagttt tatcaggcgt ccgcgactta
    11161 actgtgtttc gctttccagc atctgcaaac gagcgcccag ggtgatcaat tccattgcca
    11221 gctgaatatc ccgcgcttcc tgaacaatgc ttttttcact catgatcagg cccttttctt
    11281 gcgcagcgct tcttcaggct gattaacatc attcagcaag cgtgttgaga gcatgatgcc
    11341 ggtatgaatt tgctggagat cgtcaacgcg ggaatcttgc gtcaactgag taatcgtctg
    11401 gtggctgtca aaacggaagt gacaaaccag ttgattggtt tctgccagct taaccatttg
    11461 cggaagagtc agtgccgcta acgttgtcgc catttcttca tttatgccga gacgaaacat
    11521 agcggacgct ttgtcctgaa caatcaaacg ctgtgcaagt agtaaatatg acaagttgat
    11581 gtcataaatg tgtttcagca actcggaggt atgcattatt cccacccaga ataaccaact
    11641 ttatttttat gcggtttcac cgcaccccgt gatgtcgccg ggaagccccg gtaaaaaata
    11701 attagcatta gaatagttgc gataagctgc aataagcaga accacctttt tggtttaata
    11761 tgtccttaca aatagaaatg ggtctttaca cttatctaag atttttccta aatcgacgca
    11821 actgtactcg tcactacacg cacatacaac ggaggggggc tgcgattttc aataatgtgt
    11881 gatgcagatc acacaaaaca ctcaattact taacataaat gtataagtca tacttttgtt
    11941 ttgggtgtat ttcaatctgt taaaaagttt ttcgctacgc tagcaagcaa aaatgaaaca
    12001 ggaataatcg aaatgggatg ttgcgcacag ttaaaataac tcaccgtaaa taatcatctg
    12061 ctataaataa tcactttcat gcaataccag ataagctatt tttaaacaga cacttaccgc
    12121 acaacaaact aattaaatac aaagaaaggc aacaatcaac ttaatgttaa tgaaatgttt
    12181 tacatatatt aaccacttgt ttaatgcgga gaatcagtac actattttct tataattaca
    12241 tttgaaatta tatgatcacc gggagtgatt gattaattgt ctttactaat aatcagacta
    12301 atatttacct gtttgaccga gttgggattg cgtcgtttct ccattaggag taaagcttta
    12361 atgtcacctg aagttcacag aataaggaac aggttatgag ctatagcaat attcttgtcg
    12421 ctgttgcggt tacaccggaa agtcagcaac tgctggcaaa agcggtatct atcgccaggc
    12481 cagtaaaggg acacatcagt ttaattactc tcgcttccga cccggaaatg tacaatcaat
    12541 tagccgcgcc gatgcttgaa gatttacgca gcgtgatgca ggaagaaacg caaagctttc
    12601 ttgataagtt aattcaggat gcggggtatc ccgttgacaa gacgtttatt gcctacggtg
    12661 agttaagcga acatattctg gaagtatgtc acaaacatca tttcgatttg gtgatttgcg
    12721 gtaatcacaa tcacagtttc ttttcgcgag catcctgctc ggcgaaaaga gtgattgcct
    12781 caagtgaggt cgatgtgctg ttagttccac ttacgggtga ttaaccgctc ctacgcaagc
    12841 tttggaaagg tagcaacttt atcgcgctgc tggctttccg cgcttcgcgg aactatctgc
    12901 tttaggtcgc taatgaagca ctcctgccag tggttaatat cgtttttcac gataacgtcc
    12961 agcatttctg catgacggga aatacgttcc gccagcgaca tagtcaatgc acgatccagc
    13021 gcagctgcaa cttcgtcacg atcgtagggg ttaacaatta acgccgacgt taactcgttt
    13081 gccgctcccg caaattgcga aagaacaaga acgcccgggt tggctgggtc ctgagcagca
    13141 acatactctt ttgctaccag gttcatcccg tcacgcagtg gcgtcactaa gcccacgtca
    13201 gagtagcgga atattttcat cagtaattta cggtcaaaat gctgattcaa ataataaagc
    13261 ggcgtccagc ctaattgccc gtatttacca ttaattcgtc cagcttcatt ttcgagctga
    13321 tgacgaatat cctgataggc ttgcacatca ccacgcgacg ttggtgcaat ctgggtataa
    13381 cgaattttac catgatgctg cggatatttt tccagcaacg cttcataggc gagaaaacgc
    13441 tctggcaaac ctttggaata atccagccgt tcgacagaaa agatattttg tacgtttttc
    13501 agttccgctt taagttgcgc cagttttggc ggcagtggcc cggcagcctg tttggctatt
    13561 tctttcggtt caatgccgat cgggtagact tctgttcgaa atgctttgcc ccaggctgta
    13621 tggctttttg cgctacgtgt cgtgacgcgg gtcaggttag aaagacaatc caggaacgcc
    13681 agacgatcgt tttctgtctg gaaacccagc aaatcataat cacaaagctg ttcaagcaag
    13741 gtgtcatatg tcggcagcgc gttgaagatt tccggtgtcg ggaaaggaat atgcagaaag
    13801 aaaccaatgc gattattcac tccccgtttg cgtaattcat gcgcaaatgg caacaggtga
    13861 taatcgtgga tccagataat gtcatcgtct tgcaacagcg gcagtaattt atctgccagc
    13921 aacgcattta cgcgtagata gccgtcccag gcaggacgct gaaattgcac cagatcgagc
    13981 cgataatgaa aagcgggcca gagaacggca ttggagaatt ggttgtagta ttcgtcaagg
    14041 tcctgttcgc tgaggttaaa agaggcccac gtaatgttac cttttttcac cttttttagc
    14101 ggctgatcct cattccctgt ttcaccactc cagccaaacc acagtccgcc tgcggctttc
    14161 agtgccccca gtatgccaac ggcaaggcca ccggcactgg cggcgtgctc gtctggtggt
    14221 gcaatccggt tagatactac gactaaacga ctcatagtca tcactcctgt tattttctct
    14281 tttttgttgt aatgcggtgg ttatcatttc aagccagctc cagacatccg gcacacccgc
    14341 cagtcgccat gatgcctgag ttgcacctgt gccaattttt actgacattc cgcccagtcg
    14401 gttaacgact gcgaagccag attcatcggt taaatcatcg cccagaaata cgggcgttcg
    14461 cccgataaag ggagcttcct gcataaaagc tgcaattgcc tcacctttac tggtacctct
    14521 cggtttgatc tcgacaacac actttccctg ctgtaacgcc atttgtggcc agatctgagt
    14581 aatacgttgc gctaatgtca ttaatgcgtc ttcatgctgc ggagcctgac gataatgcag
    14641 cgcaaaagcc atccctttcg cctccagctc cgcgccggga tactgagcga tgactgtatg
    14701 cagttgcacg ctaatatcac gcgcaatcgc atccggcaga tgaacgatat gtgttttacc
    14761 attgatgtca cggcgctccg ccccatgcac gcccgctaac gggaagcgat aaggttttgc
    14821 cagtgcgtca agctccacca ttgagcgccc tgatatcaat gccaatgcac catcacttgc
    14881 ggttgccagt agctgtagtc cttgcagaat attgtcaggc acgacgacct gatcgggatg
    14941 cggtttgatt tccgccagcg ttccatcaag atcaaaaaac caggcatatt tcgcggatag
    15001 ttcaggggtt tcggttaacg gttctgtcac ccggctctcc tccttctctt ttcttattgt
    15061 tttgcgcaga acgcgcttta cagacatgta agtatagaca gtgtgacggg ggtcgccatt
    15121 tgcaatgaag aacagccaac cgttgtggcg gctggctgtg gtgggaaaaa acgttaaatt
    15181 gttgtggaaa aaagcatcag acagtgcgtt tcgctttttg cttgtaacgg tcgaagatca
    15241 ccgctgccag caggattaag ccgcgaacca cgtactgcgc gaaaggagaa atattaagca
    15301 ggttcatggc gttttccacg gtgcctaaaa ttaagatacc cgccaccaca tatgagattt
    15361 ttccgatgcc acctttcaga gaaacgccac ctaaaacgca ggcggagata acaataagct
    15421 cataaccaat cgacgtcatt ggctgcccac tggtcatacg tgaagccaga ataattccgg
    15481 ctatcgcaga taccaggcca gagagaacaa agataataat tttggtgcga acaaccggta
    15541 cacccgccag acgcgcagcc tcttcgttcc cgccaatcgc cagggtgtta cgaccaaagg
    15601 tggttttatt cagcaacaaa ccaaagataa tcagacacgc gacggtgagc cagattggcg
    15661 caggcagacc gaaccagtta gcgtaaccaa gggcaaagaa gctttcatct tcgataccga
    15721 ccgctttacc gtctgaaatg atgtacgcca gacctcgaac aatctgcatc gttgccagtg
    15781 ttgtgatcag agcatttatt ttcagtttgg cgataacaaa gccattgacc aggccacaga
    15841 gaatgccaag cagcaacccc gctgccacgc caatccacag gctttcagtc aggttgataa
    15901 ccaccgccgt ggtgacaccc gcacaggcaa ttacggaggc gacagaaagg tcaaagtcac
    15961 cggaagcaag gcagaacaac atgccacacg ccaccatccc cgacatggaa attgccaggc
    16021 ccaacccttt catattaatg aaggtggcaa aatttgggac aaaaatggca caggcgataa
    16081 agagcaccgc aaacaccacc agcatgccgt actgatccca gatacgcccg aagctgaatg
    16141 acgacttagg tgcgccagac cccgatgtag aaacagaaga catcatactc tccttactca
    16201 ggcaactgcc tggctgactt taggcatcgc aaggctcagt gcctgacgct catctgcctg
    16261 ctcgtgtaac aattcaccgg cgatttcacc ttcccgcatc accacaatcc ggtcggcaac
    16321 gccgaggact tcaggtaagt cgctggaggc aaacagcacc gccacaccct gcgccgccag
    16381 cgcataaatc acgttgtaaa tttcatgctt agcaccaaca tcaatgccgc gcgtaggttc
    16441 atccagcaaa atgaccttca tctcttccga taaccagcgg cccagaatgg ctttttgctg
    16501 atttccgcct gagagattca tgatcagttg ctcagcgccc ggcgttttga tgttgagcga
    16561 acgaatgtgg tgatcggcat tgttttcttc ccaaccgttg ttgattacac aaccgccgag
    16621 cacatgttta cgtctggcac tgatgttgat attgtcgcga acggagtgca cgggaataat
    16681 gccttccgct ttgcgatctt ccgggcagag catcatgcct gcggcaatgg cgtggctcgg
    16741 tttacgaata tcgatcggct gttggtcgat ataaacctga ccggcggtga tttgcgtccc
    16801 gccaaacaag cctttcatta attcgctacg ccccgctcct accagaccaa acagaccgac
    16861 aatttcacca ctgcgaaccg ccagacttat tggcgtacgc acgcctggtg ctttcacagc
    16921 atcaagacgc aggcgctcct cgccataact acgaggttgc cagccgtaga tatcgccaat
    16981 gtcgcgcccg accatcgcct gcaccagcgc gtcgtggtca acctgctgca tatcggtaaa
    17041 ggttttgaca taacgtccat ctttaaagac ggtgatggca tcgctgaggg caaatatttc
    17101 ttccatacgg tgagaaacgt ataagatcac ccgcccctct tttcgcagtt cacgaataac
    17161 gcggaaaaga ttgtcgattt cgcgggcaga aagggagctg gttggctcat caaaggcgat
    17221 aattttggcg ttacgcgcca gcgccttggc gatttcaacc atctgccact gaccaatgga
    17281 gagatatttc agcggcgtgt ccgggtcaat atccatacca agatgtttaa gttgtaaacc
    17341 cgcctcataa ttcagcaatg agcgattcac aatgccgcct ttatgcggca gctggccgag
    17401 atagatgttt tccgcgacgg tcatttccgg cacgagatgc agttcctggt aaataatcgc
    17461 cacacccgca ttaagtgctg cggtcgtgtc ggaaaaggac atttcctgcc cattaatcac
    17521 tacagaaccc gtggttggcg catagttgcc gctgaggatt tttaagagag ttgattttcc
    17581 tgcgccattt tcacccatca acgcatgaac ctgaccggca tagcaatcaa aactaatatc
    17641 cgtcagcgcc ttaacgccgg gaaatgtttt accgatgccg cgaaatgaga gatacggggt
    17701 agactgttgc ataacgtctc cgtgaatcac tggtcgttca caccccctct gcatcatgca
    17761 gaggggaatt tttccggcaa attacttacc gcctaaacct tttttctcca gttcttcttt
    17821 aaagttgtca cgcgtgatca gtaccacgtc ggtaacttcg gtaaattttg gcggttcaac
    17881 gtcttttgct acccagttgt aaagcatttc gctggattta tagccatgta cgtccgggct
    17941 tggcagcagg gaaccgtaga agccggttgc ctgtgcttta gacagttcgc tcaccgcatc
    18001 cacaccgtta atgccaatgc cgatgatatc ggccgcttta aagccctgac cttccgtcgc
    18061 gcgtacgccg cccagcacgg tgctgtcgtt cataccgacg atcagccaat gtttaacttc
    18121 cggatgttga accagcattg agttggcagc gtcaaatgcc cccgggatgt cgttagattt
    18181 ggtaggtacc tgataaattt gtttttccgg gaatccggcc gctttcagcg catccataga
    18241 tcccgtagta cggcggcggg cggtatccag ttcgttggcg gtaatcgcca tcaccgcgct
    18301 ttctttgaca tcccagccac gtttctgcat ctctttatac agttcctggc cctgacgttc
    18361 gccaatttta gtcgccgcca tcatcaccag cggaacggta tccattggct tacctttggc
    18421 gttaacaaac tggtcatcca cggcaatgac tttcatatcg tagccacgcg ctttcgcgac
    18481 gatggcagag ccgagtttgg ggtccggagt acaaataacg aaaccttttg cgccactggc
    18541 agccaggctg tcgatcgcgt tcaatgtttt ttcgccatcc ggcacggcaa tcttaataac
    18601 ctcaaaccct aaatccttcc cggctttatc ggcaaacttc cattcggtct ggaaccacgg
    18661 ctcttccggt tgcttcacca gaaaaccgag cttcaggttc tccgccatag cggattgtga
    18721 cataacggct gccagaccaa tggctgccag ggctttagta aatttgtgca tggttctctc
    18781 cagctttagt gtcgttttgt gtagggcaaa aacgaatgac attcgttaaa ttaatcggaa
    18841 aacaaagcat taccttttaa ctaaaagata agtgaccgtg ttgacatagt tttagcgaga
    18901 aattaattct ccataggaga gcaatatcac atcgcagaat tacagtgaga acgtgcataa
    18961 atttagcggg aaaagacata aggaaaagcc aatttgtctg acaaattgtc tagtgcacag
    19021 cagattaatc cataagatta gcctggaaat ccttgttgtc tttggtaccc agcgggatgt
    19081 ctttttttta accagtcaat aggccacatt acctggcgtt gagtttttgc aatggtgtaa
    19141 taaccgcaac tcaaagatgt ggaaaaagcg cgtcattcat ttcgccatta attatcaccg
    19201 tgttcattaa ttaacagaac acgtatactg agagccatat cgcaaaaatg aaaaaacgtt
    19261 ttaaaaaatc attgcttcat catgaattca aattcaccga ttaatatcaa caagatacaa
    19321 aaagcactat cattaaaatt cattgcagtt acattgattt catcaatgaa atgtaaaaat
    19381 atataaactt gatgatttaa gcattttctt atacccattc agacgttatt cttatttcag
    19441 atcatcgtca gaattgactc cacgatcaca tttcggaccg gcagaaagga attattctgc
    19501 aaacagtaat tatggtgttt tgatttatct tgcacctctc cacttctgga tataaggata
    19561 ttaggtatgg caaccgctgg aatgcttctc aaactcaact ctcaaatgaa ccgcgagttt
    19621 tacgcatcca atctctacct tcacctgagt aactggtgtt ctgaacagag tctgaacggc
    19681 accgccactt tccttcgcgc ccaggcacag agtaacgtga cccaaatgat gcgcatgttt
    19741 aactttatga agagtgtcgg cgctactccc atcgttaaag ccattgatgt tcccggtgaa
    19801 aaactgaact cactggaaga acttttccaa aaaacgatgg aagaatacga gcaacgttca
    19861 agcacgctgg cacagttagc cgatgaagct aaagaactga atgatgattc aaccgtcaat
    19921 ttcctgcgcg atctggaaaa agaacagcag catgatggcc tgttgctgca aaccattctt
    19981 gatgaagtgc gcagtgcgaa acttgctggt atgtgccctg tgcagactga ccaacatgtt
    20041 ctgaatgtcg tgtcgcatca gctgcattga tcgtcatcgg cgctaatgca attgcgccga
    20101 tgaagttttt gagaaaccgc tgcctcatct ttttgaagca gcggtttttt aatgggattc
    20161 gccctgtggg gtaaacttga gttcaatcag cgcgatggct ttttggattg cccgcatggt
    20221 gaccgggtct gcggcggcgg gatggtgagt aaagtcgata ttcttcagct ggctggacat
    20281 tttttcgcga acttcaacgg gcgcgattac atcgagaaca tcctgaattt gtttgataac
    20341 caactggcaa gcaaccacat cagaaaccag ttcctgatcg gcattcagcg gctgggacat
    20401 cgtaaactcc tgatagcatt ttgaaagccg ttatagtagc ggcttcacat cttcagcgat
    20461 agtcacatcc accgtcacca ggacacaaaa aaacctgccg gagcaggttt tttgttatcg
    20521 gaacatattg cctggtggta cgtctttgaa cgtcttgcaa tagttattga acatactttt
    20581 caggatcttg cgcagtttca tcgcggcact ccgactattt gttatgcagg tgttattgtc
    20641 tttgcactta taatatgaca accatcacaa aaatcaatct ttatgtgatg caaatcacat
    20701 aaataccctt ttaatgttat aaaaatgata atcaaaaagc agcaccccca tttctgacac
    20761 ctacagatgg caagaaatag cgcctgccag gcgtcttttc cggccattgt cgcagcgctg
    20821 taacgcgtaa aatagtgctt tcttttactc ttctggctgg accatgagac ttctgattct
    20881 gactctttca ttaataacgc tggcggggtg cacagttact cgccaggctc atgtgggcga
    20941 agttgacacg gcaacaggta ttgttcggtt ggtttatgat caggcttttt tgcagcatgc
    21001 ccataccgat cgttatgtca gtcgcgggat cgccgatcgc gcatgccagc aggaaggcta
    21061 tacccacgcg atcccctttg gtcagccagt aggcaactgt agcctctttg cgggttcctt
    21121 atgtttgaat acggaattca ctttgtcata tcagtgccat cattccgcct tccctgtctt
    21181 tctctaaacg ttttaatcag cgagggggat cttcgctgat taaataaata gcggtaatgc
    21241 gttttattcc cattcgtatt tttaataaat gatgtttata ttttaccttt tgcaaataat
    21301 aaaataacaa attatagtga cgccacagca acaaatataa cctttgtgga gcactatcat
    21361 gctgaaacca gaaatgattg aaaaacttaa tgagcagatg aacctggaac tgtactcttc
    21421 actgctttat cagcaaatga gcgcctggtg cagctatcat accttcgaag gtgctgccac
    21481 gttcctgcgc cgccacgccc aggaagagat gacgcatatg cagcgtctgt ttgattatct
    21541 gactgatacc ggcaacttac cgcgtattaa taccgttgaa tctccgtttg ctgaatattc
    21601 ctcacttgat gaattattcc aggaaaccta taaacacgaa caattaatca cgcagaaaat
    21661 taacgaactg gctcatgctg caatgaccaa tcaggactac ccaacattta atttcctgca
    21721 atggtatgtt tctgagcagc atgaagaaga gaaactgttc aaatcgatta ttgataaatt
    21781 aagcctggca ggcaaaagcg gcgaaggtct gtattttatc gacaaagaac tctctaccct
    21841 cgacacacaa aactaatgct ctccggcggc aggtttactg ccgccgtttc aaatcagtga
    21901 cggcaaatct tactctgatc ggtcgaaaaa ccgtcttccg aaggtataaa tttgcctttt
    21961 gctgccagaa acgctaccag ctctcccgca gtcatccctt ctgccgagca ggtgtgaaaa
    22021 cgtgcctgtt caccaaaacg cgctttaatc gcagcttcca gactggcatg cgtatattgc
    22081 tcgcctgatt caatcatcat atttaacact tcatgaccgt gaatagagtc catcgttcct
    22141 cctcaaaaaa aagcctagcg tagcgattac cgcttatgaa gactttgcgc cagcgcagga
    22201 ctgaatgctt ttattgtaca tttatattta caccatatgt aacgtcggtt tgacgaagca
    22261 gccgttatgc cttaacctgc gccgcagata tcactcataa agatcgtcag gacagaagaa
    22321 agcgtgaaaa acagaaccct gggaagtgtt tttatcgtgg cgggaaccac aattggcgca
    22381 ggcatgctgg caatgccgct ggctgcggca ggtgttggtt ttagcgtcac gttaatcttg
    22441 ttgattgggc tttgggcgtt gatgtgctac acggcgctat tactgctgga ggtgtaccag
    22501 catgttccgg cagataccgg tcttggcacg ctggcaaaac gctatctggg acgctacggt
    22561 caatggctga cgggcttcag tatgatgttc ttaatgtatg ctctgactgc ggcatacatc
    22621 agcggtgccg gtgaattgtt ggcctccagc atcagcgact ggacaggtat ttctatgtcg
    22681 gcaaccgctg gcgtgctgtt gttcactttt gttgccggtg gcgtggtttg tgtcggaact
    22741 tcactggtcg atttatttaa ccgttttctg ttcagcgcca agattatttt tctggtggta
    22801 atgctggtac tactgctgcc gcatattcac aaagtgaatc ttttaaccct gccgttgcaa
    22861 caggggctgg ctctgtctgc aatcccggtg atttttacgt cgtttggttt tcacggtagc
    22921 gtgccgagta ttgtcagcta tatggatggc aacattcgta agctacgctg ggtgtttata
    22981 atcggtagtg cgatccccct ggtggcatat attttctggc aggtggcgac gcttggcagc
    23041 attgattcaa caacctttat gggattgctg gctaatcatg ctggattaaa cgggctgtta
    23101 caggcgttac gcgaaatggt ggcctctccg catgttgagc tggcagtgca tttatttgct
    23161 gatttagccc tcgccacgtc atttctcggc gttgcgttag gcttatttga ttatctggct
    23221 gatttatttc agcgttcaaa taccgttggt ggacggttgc aaactggtgc aattacgttt
    23281 ctgccgccgt tggcgtttgc actgttttat ccacgaggat ttgtgatggc gctgggttac
    23341 gccggtgtgg cgctggcggt actggcattg attatccctt cgctgttgac ctggcaaagc
    23401 agaaagcaca atcctcaggc gggttaccgg gtcaaaggtg gtcgtccggc gctggtggtg
    23461 gtgtttctct gtggtattgc tgtgattggc gtgcaatttt tgattgcggc agggttgtta
    23521 ccagaagtgg ggtgatcaga tagcctcaaa ttccttattg ggtgccagaa ttaacgctga
    23581 cacccaattt ggcctcttaa tgcaggcagc actgcttaaa tttcttacca ctaccgcacg
    23641 ggcaaggatc gttacgcccc ggtttctctt ctgctttgat cggttgctga acagcttttt
    23701 cctgcggatg cgccatccag tacgcatgta gatcaagcgc cgccagtcga atggcatcta
    23761 cgctctcttc aaacgcttct ggcgacatct tttctacccg ctcgaagttt tcctcagtac
    23821 cgtgcagcgc aatcgcctcc agcgctggtt ttaacgaatc gggcaacgtt gaccagtcag
    23881 aaagtgccac gccccgcata taaccaaagc accactcctc aacaatcgtc agctcgctgc
    23941 catcaacttc tcgcaagccg aataacggct caaactgctc cgggaattcg ttcagacgct
    24001 ctgcggtatc ggccatatgt tgaaaagcca gattcataaa gcgcgtcatc tctttctctg
    24061 acgcccagcg cggcacatag tcagccccac cccacacggc aaccagccac tgttccggtt
    24121 caatctcttg cggagaactc aacaccgccg tcaataaacc gtccagctcc gccacatcaa
    24181 ggatggcgtg gtcagtgttg tatttggtca gaatatcgtc cagccattcc aactcacttt
    24241 cgtttaacgg tcccgttttc atacgctttt ccttgtggat ctcaactcgc cagcacctat
    24301 cttacatgcc ggtccgtatc agagatactt tttgagtggc tttgctggtg attaaaaatt
    24361 aaggagggtg taacgacaag ttgcaggcac aaaaaaacca cccgaaggtg gtttcacgac
    24421 actgcttatt gctttgattt tattcttatc tttcccatgg tacccggagc gggacttgaa
    24481 cccgcacagc gcgaacgccg agggatttta aatcccttgt gtctaccgat tccaccatcc
    24541 gggctcggga agaaagtgga ggcgcgttcc ggagtcgaac cggactagac ggatttgcaa
    24601 tccgctacat aaccgctttg ttaacgcgcc aaattcttca ggcctttcag ccagacatcc
    24661 gcttgacgcc gatgtctttt aaactggagc gggaaacgag actcgaactc gcgaccccga
    24721 ccttggcaag gtcgtgctct accaactgag ctattcccgc attcatcaag caatcagtta
    24781 atcacttgat tttattatcg tctggcaatc agtgccgccg ttcgatgcgt tgcattctac
    24841 ttacctggcg cgatgagtca acgatatttt tcaccacttt tgatcgtttg ctgaaaatta
    24901 cgccgaaacg atcactgatc aagcaaatct gcacgcgcag cgctcaaata ttgcaacatt
    24961 gaccacagag tcagtaccgc agccacaaag aaaagtgcaa taccggcgta ctcaacccaa
    25021 atgttcggac gccacagcag ccatgccaac gccaccatct gggccgtagt tttcactttc
    25081 ccaatccagg agacggccac gctactgcgt ttacccaact ccgccatcca ttcgcgtagc
    25141 gcagaaataa taatttcacg ggcgatcatc gttgccgccg gtaatgtcac ccaccagctg
    25201 tgataatgct cggttaccag caccatggcg atagccacga gaactttatc tgccacaggg
    25261 tcaaggaaag caccaaaccg ggtactctgg ttccagcggc gtgccagaaa accatcgaac
    25321 cagtcagtca ccgccgcgac gcagaaaatg agcgcggcgg caaacggcga ccaggtgaca
    25381 ggcagataaa agaccaatac aaagaatggg ataaggatga cacggaacag tgtaagcaac
    25441 gtagggatat taaattgcat aatgacgggt aactatctgt tgtcagtaag attaccccta
    25501 tgttgctaca gagacatcaa tgtttcaacg accagaagat cttttctgcc agaccttgcg
    25561 aaatacccgg cacttttgca atttcctcga cgctggcgtt acgtaaacct tgcaaaccgc
    25621 ccatatattt caacaacatt tgccgacgtt ttggcccgac gccttcaatg gtttccaggg
    25681 aactggtatt tttgaccttc gcccgttttt tacggtgccc gccaatcgcg tgatcatgtg
    25741 attcatcgcg aatatgctgg ataacatgca gcgcgggaga atctggcggc aaactaaatc
    25801 cctcaccttc cggctcaaag aacagcgttt ccagcccagc cttacgatct gctcctttgg
    25861 caacgccaag tagcagcgga tgatttttat cccatgagac atccagttcg gcgaagacat
    25921 ttttcgcctg cgcaagctgg cctttgccgc cgtcgataag gatcacatca gggatcttac
    25981 tgtcgtcaat ggctttacca taacgccgac gcagcacctg attcatcgcc gcataatcat
    26041 cgcccggcct gatgcctgta atgttatagc gccgatactc cgcacgcagc gggccgttag
    26101 catcaaacac cacacaggaa gcgacggttt gttcacccat ggtatggctg atgtcaaagc
    26161 actccatccg cttcacttcc ggcaatttca acacgctggc aagcgcggtc agtcgctggt
    26221 gaacggtaga ttgctgcgaa agtttgctgg ttaaggccgt cgccgcattg gtgcgcgcga
    26281 gtttcagata acgcgcccta tcgccgcgag gtttggtttg aacattaatc ttgcgtcccg
    26341 ccagttctga aagggaatcg gcgagcagcg ttttatcgct aagattaaaa tcgagcagga
    26401 tctcacccgg taaggtgcgc atctggctgc cttgtaaata gaactgtcct acgaaggttt
    26461 ccaccacctc gctcagttcc gtaccgccag gcactttcgg gaaatagctg cggctgccaa
    26521 gcactttgcc ctgacgaatg aacaatacgt ggacacaagc catgcccgca tcgaacgcca
    26581 caccaataac gtcgaggtcg tcgccggtat tggacacaaa ctgtttttcg gtgacgcgtc
    26641 gcaccgcctg aatttggtcg cgaatacgtg ccgcttcttc aaactccaga ttctggctgg
    26701 cagtttccat acggctaatc agttgcgtaa gcacctgatc atctttgcca gacaaaaaca
    26761 ggcgcacata ctcgacctgc tgagcgtatt cttcttcact caccagtcct tcaacgcacg
    26821 gccccagaca acgtcctatc tgatattgca gacacggacg cgagcgattg cgataaacac
    26881 tgttttcgca ctggcgaatg gggaaaatct tttgcagtag cgccagtgtt tcacgtacgg
    26941 catagccatt cgggaacggg ccgaaatatt cacctttggc atgctttgca ccacgatgca
    27001 tcgccagacg cgggtgggta tcgccactca ggaagataaa aggatatgat ttatcatcgc
    27061 gtagcaaaac gttgtaacgc ggctgataga gtttgatgta gttgtgttcc agcaacagcg
    27121 cttcggtttc tgtatgagta accgttacat caatttgctg gatctgggcg accagcgctt
    27181 cggttttgcg cgaagcgagg ttgctacgga aatagctgga aagccgtttt ttcaggtctt
    27241 tcgctttgcc gacatagata accgtaccac cagcatcgta catgcgataa acgcctggct
    27301 ggctggttac ggtttttaaa aacgcttttg cgtcaaactg atcactcact gacttgataa
    27361 tgtctccgca ttacacagac catggcgaat tgccaggtga gtcagctcaa catcgccatg
    27421 aatgtttagt ttactgaaca tacgatagcg gtagctgttc accgttttcg gactgagatt
    27481 gagctgttct gagatctcat tgaccttctg gcccttggtg atcatcagca taatctgcaa
    27541 ttcacgttca gacaaactgg caaatgggct ttctgttttt tctggttcga tctggcttaa
    27601 cgccatttgt tgagcgatgt cagaagcaat gtaacgctgc cctgaataga cagaacgaat
    27661 cgcactcacg acttcctgcg gagccgcgcc tttgctgagg tagcccgcag caccggcctg
    27721 catgactttc gctggtaaag ggttttctgt atggacggta agcatgatga ttttgacatc
    27781 agctgtggaa cgcgcgattt tacgcgtcgc ctcaagaccg ccaatgcccg gcatactcat
    27841 gtccattagc accacgtcaa cggcatttgt ccggcaccac ttaacggcgt cttcaccgca
    27901 cgatgcctca ccgacgactt ttataccctt tatatcttcc agaatgcgtc gtatccctgc
    27961 gcgcaccagt tcgtggtcat caacaagtag aacgttgatc aaaggaatat ctccagaaat
    28021 agggataacg ctactgatag ttagtcattc gtatattagc ggtttttatt gcaactttga
    28081 aacgttaaaa atggtgggta ttcgattttt ctctcgtttt tgtcaattcg attgtccaca
    28141 gttatggtca cgcccgtcac caacggctta ccggatcgtt tcacagtaag ccctacgatg
    28201 aaatgtgaca aaaattgact ttattcagca aaaatgaaaa tcagcccgct gaatatgtaa
    28261 cattaattaa cccgaacaat acgggcaaaa aacattcatg ataaaaatat ttattggtca
    28321 ttatattaac gtattttata gcactgccga tatcacgctc aaaaaacaac cactgctatt
    28381 tttagcaaag cttatggtat actccgccgc cttaacattt ttcaccgcaa attttcattg
    28441 caacatgacg aggaaaataa atgagtacgc ctgatttttc tactgccgag aataatcaag
    28501 aactggcaaa tgaagtctcc tgcctgaaag cgatgctgac gctgatgctg caggcgatgg
    28561 gacaagctga cgcgggccgc gtgatgttaa agatggaaaa acagcttgcg ctgatcgaag
    28621 acgaaaccca ggctgcagta ttttccaaaa cggttaagca aattaaacaa gcctaccgtc
    28681 agtaatataa aaccggctga gagcgtgcct ttcagccggt ttttgcatct ggcacgcaga
    28741 aagaaaagcg atcaaattaa gccagtagcg gccgcgtaac aggccacctg ggtcttattt
    28801 ggtgcattga tttttttctg catgtttttc tgatggaaat tgaccgtatt ctcagagatt
    28861 gacaaaatca tcgctatctc tgctgatgtc ttcccttccg ccgtccattt cagaatttct
    28921 ttttcgcgct tgctgaaatt catctctggc gtcatcacta tttcatcatt taaacgcatc
    28981 agagccatca gactttcgcg caccagtaac tgcattttta attgcagttc atcactaaga
    29041 atgggtattt cgcgagcgct gcaacgggaa aaggacaaaa agcccaacgc ccgatttggc
    29101 agcattaaat actgagtgac accgcggcgt aaaccatgtg cgcgcgcggc ttcccataac
    29161 ggctgtgctt cgctgaataa gtcatcattc cacattaaat ggccctgact aaagttttca
    29221 gggttcagca ccggatcaat tgcgagaaaa ttttttgcct gataataact aacccacgac
    29281 tcagggtaat tggtgtagaa agccacttta ggtcgcgtga atggtaccgg gtggcgaaca
    29341 cataacgaat agtaatcgta ctccagctgc tgagcctgaa gctctatttc atggtagacc
    29401 tcttctgcgg cctccatcct ctgaaaacgc aacagcatcg tgcgacgcca gctgaaaaaa
    29461 tccgtatcct gcatagtaaa ccgcaacgcc cctgagagtg agtatcattt ataatgataa
    29521 ttcaaactat cacataaata agatttatat ataatttata ttattcaggc aatgaattac
    29581 ttttgcaagc catcgcattc tcttatgtta ttaatgagtt atgctgattt gttaagcagt
    29641 tttatcaggc ttgaaatggc gtcccgcccc gacaggtgaa tcgtcggggc tgattttttc
    29701 ttattattgc agcagaaact tctcgaggaa ctggcgggtg cgaggctgct tgggatcggc
    29761 aaataacgct tttgcggccc cctgctcgac tatccgcccc tggtccataa aaatcgcccg
    29821 gtccgcaaca tcccgggcaa agctcatttc gtgcgtcaca atcaccatcg tgcgcttttc
    29881 ctgtgccagc tgacggatgg tgttcaggac ttcgcccacc agctctggat ccagcgccga
    29941 cgttggctcg tcaaacaaaa tcacctcagg acgcattgcc agtgcacgcg caatcgcaac
    30001 acgctgctgt tgaccgccag acaaacgacg tggatagctg gtttctttac cggccagccc
    30061 aactttcgcc agtagctcgc gagcgcgctc cgtggcctct tctttcggtt cgcctttgac
    30121 gatcaccggc ccttcaataa tgttttccag taccgtacga tgcggaaaca aattaaagtt
    30181 ctggaagaca aacccgacgt gctgacgcaa ctggcgaatc agcgattttt gctgacttaa
    30241 tgaacgtgca gtatcaatag tgatatcgcc aacggtgata gttcccgctt ccggttgttc
    30301 cagcagattt atgctgcgta gcaacgtggt tttgccggaa ccactcggac cgataattgc
    30361 cacaacttcg ccaggcttta cctcaaggtc gataccgtgc agcaccgtct gaccgtgaaa
    30421 ttttttcacc aggttcttaa cttcaatggc actcattttg gttctctctc ctggcgatta
    30481 agttgattct caaaatggtt ctgcaacgtc gataatactg tcgccatgat ccagtagatg
    30541 agcgaagccg ccaggtacat ggtgaacacc tccagcgtgc gcgaggtaat caactgcgcc
    30601 tgacggaaca gctccggcac ctggattgtc gcggccagcg acgtatcttt taccagactg
    30661 ataaagctgt tcgacagcgg cggcaacgcc acccgcgccg cctgtggcaa aatcgcgcga
    30721 cgcatagtct gccatggcgt cataccaata ctggcagctg cttcccactg acctttatca
    30781 atagaagaaa tggcggcgcg aagcgtttcg gcggcatagg cggcagtgtt cagcgacaga
    30841 ccaatcattg ctgacggaat gggatctaat tcgataccaa actgcggcag gccgtaatag
    30901 atcataaaca gttgtgcgat gagcggcgta ccacgaaaaa tagagatata aaaacgcgcc
    30961 agccagcgca cgggccagat cggcgacagg cgcatcagcg cgagaataaa cccgagcagt
    31021 aagccaaaaa acatgccgcc gatactgagt tgcagcgtat acccagcccc tttcagcagg
    31081 aacggcaagg aatcaataac cagttgtata ctttcttgca tgagcgtttc cggagctaaa
    31141 cgtggggatg ataggcgaac agcgcaggcg cgccgccggt atgaataaac agaatcggcc
    31201 cttcatcttt gaagcgtttc tgactgatac cgtcaatcag ccccgccatc gcttttccgg
    31261 tatacacagg atcaagcaga atgccttcca gccgcgccag caatttcact gcttccatgc
    31321 cttcatcgtt cggcacgccg tagccaggtg caaaatagtc atcccagagt aaaatttccg
    31381 ctgatgcggt cagctccagt tctttcgcaa tcgcctgttg taggttaacc actttcggca
    31441 attgatcggc aacggaacgc gacacggtaa cgccaatcag ttcgctttca ggcataaggt
    31501 gttccagccc aacagccagt ccggcgtgag ttccggcact gcccgatgcg actaccaccg
    31561 acgaaatatt aaccgcccct tcacactgtt gcgcgatttc cagcgcactc tccacataac
    31621 ccagcgcgcc cagagcatta gaaccgccaa ccggaatgac atacgggcga aagccttgtg
    31681 cttcgactcg cgtcgccagc tcttccagtt gggcattggg atcggtcagt gcgtcacaca
    31741 tttcaatctg ggtattgaac agatccagca acaaacgatt gccgttggtt aaatagtttt
    31801 ctgcggttgt gccaatagga ttttccagca gcgccacgca gtgcagaccg agtttcgccg
    31861 caactgcggc agtctggcgc acatggttag actggatcgc gccggcagta atcagcgtat
    31921 cggcaccttc gcgcagagca tctgctgcga gaaattccag cttacgtaat ttattgccgc
    31981 ccattgccat gggggtgacg tcatcccgtt tgatgaaaat ttcccgtcca agataatcag
    32041 aaaagcgcgg cagatattcg agcggtgttg gcgcgccgat aaactccaac cgtggaaaac
    32101 gggttaaatt atgcagtggc ataacagcct ccgatgtgtg ttgttgtgat tttcttatta
    32161 tgcacgctga aaacgcgtaa ataaaaaagg cgctagtgaa agcgcccttt tttgtcatta
    32221 tgctgattat ttggtcacat cagcaccaaa ccatttttcg gaaagggctt gcaaagtgcc
    32281 gtctttttgc atttccgcaa ttgcatcatt cactgctttc agcaggtctt catttccttt
    32341 acgcagcgcc acgccagact cctgacggga gaatgcttca ccggttactg ccagcgtatc
    32401 gttggttttc ttcaccagat ccagcgccgc cagacgatca acgaggatcg catcgatacg
    32461 ccctacgcgc agatcctgat atttggtcgg gtcatcatca taggtacgca catcgacacc
    32521 ctgaacattc tgccgcagcc actcttcata gttggtgccc agaccgacac ccactttttt
    32581 gcctttcaga tcagcagccg ttttaagggt gccttcgtta ccttttttca ccagcgcctg
    32641 aataccggaa atggtgtaag gggtagagaa atcgtatttt ttcttgcgct catcagaaat
    32701 ggtgacctga ttaatcacca catcaatgcg tttagagtcc agcgacgcca gcataccgtc
    32761 ccatttggtc ggttttagtg acgcctcaac gccaagatgt tttgccagct gttgggcaaa
    32821 ttccacttca aaaccggtta atttgccgtc atccccctga aaactgaacg gcggataagt
    32881 tccttccagc cctaccagta gcgtaccgcg ctctttgact ttattaagca gaccttcatc
    32941 tgcaaaactt ttaacgctca tgcccgcacc cagcgccacg gccatcacac ccatcaatgc
    33001 ctgacgtccc agatgtgcta atttcatatt caccccgaat gttgttatgt ctgtttgcag
    33061 tgtagagcca ttgtttgtaa acacaaaaac aactccgcta catcttattc ttatttaata
    33121 tatatcagaa gcaggcaggc tggaggattt ctgcacaagt ccggtacacg tttgacgctg
    33181 atagtgttga tacttccgta atgcgatgcg gtaattgttg gtgctggtgg tgggcagcca
    33241 cggagcgagg atttcatcaa ggaaaccgtc ctgcagctga tcgagggaaa tattttgctc
    33301 atgtagatga ttccccaaac ggcgcaaacg aacgacatat tcacgcaccg tacctgggct
    33361 catttcagtt tgctcaaaaa gaaattgctt aaagccgata atatcgaaaa aatcactctg
    33421 cgttttgcag tgtaaatcac cgcaaaatcg gcacaatgcc gcccatgatt gttgttccac
    33481 ttgccagcca ttttcatcaa gcagcgtgtc caggcgggaa atctcgattt tattcactat
    33541 tttgccgtcg cgaactaagg taatgcgatc gagtaattta cggcaatgcg cgcaatgggt
    33601 ctggctgtgt ttaaagtctt taagatagcg gcttaatggc cgtcttttca ggtgctgcac
    33661 catcattaag aactcctggt agtcaaagtt aaagtgcggc atttactgac gttataactt
    33721 ccccagttta gtgcgtaacc gtttaatagc ctggctgtgt aactgactga cccgcgattc
    33781 cccgacctcc agcactgcac caatctcttt gagattcagc tcttcctggt aatagagggt
    33841 taataccagt ttttcgcgct ccggcaacgt ttcgatggct tccatcaccc gctggcgcag
    33901 attactgtcc agcagttgtt gtagcgggtt ttctcgctga tgatcatcag taaccagttc
    33961 gatgctatcg ccgtgctctt cgcgccactc atcgtaggag aagagctggc tgttattggt
    34021 gtcgagcaac atttggcgat aatcggcaat atcgatccct aaacgttccg ctacctcagt
    34081 ttccgtggcg ttgcggccaa gttcctgctc cagttgccct attgcctgtg ccacttcacg
    34141 cgcgttgcgt cgcacgctgc gcggcaccca gtcacggctg cgaagttcat ccagcatagc
    34201 gccacggata cgctgcactg cgtaagttgt aaatgccgtt ccttgtaggg cgtcatagcg
    34261 ttcgacggca ttaagtaacc caatgccgcc cgcctgtagc agatcgtcaa gttccacgct
    34321 cgcgggcagt cgaacctgca ggcgcaatgc ttcgtgacgc accagcggga cataacgctg
    34381 ccacagcgag tgtttatcca ttacaccttc agcggtatag agtgaattca cgataaacag
    34441 ccctgcgtta tatgagttat cggcatgatt atccgtttct gcagggtttt taatcggacg
    34501 attagtgggt gaaatgaggg gttatttgag gtttaccggt aaattccagg cagaaaaaac
    34561 cccgccggtg gcggggatgt acgttgctga caaattacgc ttcatgttgc cggatgcggc
    34621 gtaaacgcct tatccggcct acaaaaatgt gcaaattcaa taaattgcaa ttccccttgt
    34681 aggcctgata agcgcagcgc atcaggcaat ttggcgttgc cgtcagtctc agttaatcag
    34741 gttacgacga ttaaccctgc agcagagaca gaacctgctg tggtacctgg ttagcttttg
    34801 ccagcacgga gttaccggcc tgctggatga tctgcgcttt cgacatgttg gacacttcgg
    34861 tcgcatagtc ggcgtcctga atacgggact gcgcttcgga caggttggta gtggtgttgt
    34921 tcaggttggt gaccgcggaa tccagacggt tctggatagc acccagggat gaacggaatt
    34981 tgtcgatgga gctgattgcg tcgtcaagcg cagccaatgg atcggacgtc ttcgatgttt
    35041 tattctctgt gctgtagaca ccatcagcgt ctttatagat ttctttgcct gcgctattcg
    35101 tgaccgtgcc atcggcttgc gagtagtagg ttgaaacagt ttgagcatcc tgggttaagg
    35161 catcccccgc caggaaacca tttgtagtat tatttgcggc aacagcataa gtattcccac
    35221 cggtgttggc ggaatatgca atgcccgtaa atgcagatgc tgaaacagta gcaccttcaa
    35281 tttttacagt acctgtatta ccttcagctg taactgttac accagcctct gtcttgatgg
    35341 ttgttttaac agcagtacct ttcgcatccg tatttccgag aagcccagaa acggttgctt
    35401 tgttcgggtt gccggcctgg tttttagtca ggttacctgt tgaatccaga taaagttgat
    35461 cgccatcact tgaagtaata ttgcccgaat catcaatatt tacattctgt gctgtcccac
    35521 caatagtaac tgatgcagct gtcgttttcc ctgctgcagg tatgagattt gctgcagcat
    35581 tcgtttggtt tacaccgtca gccgtatagg agaaggtatt gctggttgca ttataagtat
    35641 aagttgtcga tgtcgcatta tcagcaaggc tatttgcacc tgttgcggta attttatcgc
    35701 cagtcttaag cgccgcaaga atatcagatc cttttgctgc agtatttgct gtagttttag
    35761 tatatgcagt gctgccgtca gatgaagtta accctttgat atatccgtct ttaaccattc
    35821 cgtcagctgt cgccgcgaca cttgccttat ctgcgctacc attcacatta aacccactca
    35881 gccccagcgt atcagagtca attttcttca gatcaatagt gatagtctgg ccgtcattcg
    35941 caccaacctg aattttcatt gaaccgtctt tcgccagtac gttcacgccg ttgaactggg
    36001 tctggccaga tacgcggtca atttcgtcca gacgggattt gatttcgtcc tgaatggagt
    36061 ccagatccga atcggagtta gtcccggtag aagcctgaac cgtcagttca cggatacgct
    36121 gtaagttgtt gttgatttcg gacagcgcgc cttcagtggt ctgcgcaacg gagataccgt
    36181 cgttggcgtt acgggccgcc tgagtcaggc ctttaatgtt agaagtaaaa cggttagcaa
    36241 tcgcctgacc cgctgcgtca tccttcgcgc tgttaatacg caagccagaa gacagacgct
    36301 cgatagaact cgacagcgca gactggttct tgttgatatt attttgagtg atcagcgaga
    36361 ggctgttggt attaatgact tgtgccatga ttcgttatcc tatattgcaa gtcgttgatt
    36421 acgttttggg tttccacccg tcggctcaat cgccgtcaac cctgttatcg tctgtcgtaa
    36481 aacaaccttt agaatttttt tcacaaacag ccattttttg ttagtcgacg aaatactctt
    36541 ttctctgccc cttattcccg ctattaaaaa aaacaattaa acgtaaactt tgcgcaattc
    36601 aggccgataa ccccggtatt cgttttacgt gtcgaaagat aaaaggaaat cgcatggcaa
    36661 gtatttcatc gctgggagtc gggtcaggtc tggatttaag ttcaatcctt gatagcctca
    36721 ccgccgcgca aaaagcgacg ctaaccccta tttcaaatca gcaatcgtcg tttaccgcta
    36781 aacttagcgc ctacggtacg ctgaaaagcg cgctgacgac tttccagacc gccaatactg
    36841 cattgtctaa agccgatctt ttttccgcca ccagcactac cagcagcacc accgcgttca
    36901 gtgccaccac cgcgggtaac gccatcgccg ggaaatacac catcagtgtc acccatctgg
    36961 cgcaggcgca aaccctgacc acgcgcacca ccagagacga tacgaaaacg gcgatcgcca
    37021 ccagcgacag taaactcacc attcaacaag gcggcgacaa agatccgatt accattgata
    37081 tcagcgcggc taactcgtcg ttaagcggga tccgtgatgc catcaacaat gcaaaagcag
    37141 gcgtaagcgc aagcatcatt aacgtgggta acggtgaata tcgtctgtca gtcacatcaa
    37201 atgacaccgg ccttgataat gcgatgacac tctcggtcag cggtgatgat gcgctacaaa
    37261 gttttatggg ctatgacgcc agtgccagca gcaacggtat ggaagtctcg gtcgccgccc
    37321 agaatgcgca gctgacggtc aacaacgtcg ccattgagaa cagcagcaac accatcagcg
    37381 acgcgctgga aaacatcacc ctgaacctga acgatgtcac cacgggcaac cagacgctaa
    37441 ccatcactca ggacacctcc aaagcgcaaa cagcgattaa agactgggtg aatgcctata
    37501 actcgctaat agataccttc agcagcctga ccaaatacac cgccgtagat gcgggagctg
    37561 atagccagag ttctagcaat ggcgcactgc tcggcgactc cacgttgcgg acgattcaga
    37621 cgcagttgaa gtcgatgctg agtaataccg tcagttcttc caactataaa acgctggcgc
    37681 agattggtat cacgaccgat cccagcgatg gcaaactgga actggatgcc gacaaactca
    37741 ccgctgcact gaaaaaagat gccagcggcg taggtgcatt gattgttggc gatggtaaaa
    37801 aaaccggcat cacgaccacc atcggcagca acctgaccag ttggctttcg acaacgggca
    37861 ttattaaagc cgctaccgat ggcgttagta agaccctgaa taaattaact aaagactaca
    37921 acgccgccag cgatcgcatt gatgcgcagg tcgcgcgcta taaagaacaa tttacccaac
    37981 tggacgtttt aatgacctcg ttaaacagca ccagcagcta cttaacgcag cagttcgaaa
    38041 acaacagtaa ttccaagtaa tcaatattca tcgggagaca ggtcatgtac gcggcaaaag
    38101 gcacccaggc ctatgcacaa attggcgtcg aaagcgccgt aatgagcgcc agccagcagc
    38161 agctggtcac catgctattt gatggagtgc taagcgcact ggttagagcg cgcctgttta
    38221 tgcaggacaa caatcagcaa ggcaaaggcg tctctttgtc aaaagcgatc aacatcattg
    38281 agaacggact gcgggtgagt cttgatgaag agagcaaaga cgaattaacc caaaacttga
    38341 ttgctcttta tagctatatg gtcaggcgtt tgctgcaagc caatttacgc aacgatgtct
    38401 ccgcagtcga agaagtggaa acattaatgc gcaatattgc cgatgcctgg aaagagtcgt
    38461 tactctcccc ttctttgatt caggacccag tctgatgaac catgcaccgc atttgtattt
    38521 cgcctggcaa caactcgtcg aaaaaagcca gctcatgtta cgcctggcaa cggaagaaca
    38581 atgggacgaa ctcatcgcca gcgaaatggc gtatgttaat gcggtgcagg agattgcaca
    38641 tttgactgaa gagattgacc cgtccaccac gatgcaggag cagctccgcc cgatgctgcg
    38701 cctgattctc gacaacgaaa gcaaggtaaa gcagttatta cagattcgga tggatgaact
    38761 ggcgaaactg gtcggtcagt catcggtgca aaaatcggtg ttaagtgcct atggcgatca
    38821 gggcggcttt gtgctggctc cgcaagataa cttatcgtga atcttaacga gctaatggct
    38881 cgcgaataat ccgattacgg ctacgcttca aatgttcccc ttgaatggag tcgaagaatg
    38941 cgtaatccca cgctgttaca atgttttcac tggtattacc cggaaggcgg taagctctgg
    39001 cctgaactgg ccgagcgcgc cgacggtttt aatgatattg gtatcaatat ggtctggttg
    39061 ccgcccgcct ataaaggcgc atcgggcggg tattcggtcg gctacgactc ctatgattta
    39121 tttgatttag gcgagtttga tcagaaaggc agcatcccta ctaaatatgg cgataaagca
    39181 caactgctgg ccgccattga cgccctgaaa cgtaatgaca ttgcggtgct gttggacgtg
    39241 gtagtcaacc acaaaatggg cgcggatgaa aaagaagcta ttcgcgtgca gcgtgtaaat
    39301 gctgacgacc gtacgcaaat tgatgaagaa atcattgagt gcgaaggctg gacgcgttac
    39361 accttccccg cccgtgccgg gcaatactca cagtttatct gggatttcaa atgttttagc
    39421 ggtatcgacc atatcgaaaa ccctgatgaa gatggcatat ttaaaattgt taacgactac
    39481 accggcgaag gctggaacga tcaggttgat gatgaattag gtaatttcga ttatctgatg
    39541 ggcgagaata tcgattttcg caatcatgcc gtgacggaag agattaaata ctgggcgcgc
    39601 tgggtgatgg aacaaacgca atgcgacggt tttcgtcttg atgcggtcaa acatattccg
    39661 gcgtggtttt ataaagagtg gatcgaacac gtacaggaag ttgcgccaaa gccgctgttt
    39721 attgtggcgg agtactggtc gcatgaagtt gataagctgc aaacgtatat tgatcaggtg
    39781 gaaggcaaaa ccatgctgtt cgatgcgccg ctgcagatga aattccatga agcatcgcgc
    39841 atgggacgcg actacgacat gacgcagatt ttcactggga cgctggtgga agccgatcct
    39901 tttcacgccg taaccctcgt tgccaaccat gacacccaac cgttgcaagc cctcgaagcg
    39961 ccagtcgaac cgtggtttaa accgctggcg tatgctctaa ttttgttgcg ggaaaatggc
    40021 gttccttcgg tattctatcc ggacctctac ggtgcgcatt acgaagatgt cggtggtgac
    40081 gggcaaacct atccgataga tatgccaata atcgaacagc ttgatgagtt aattctcgcc
    40141 cgtcagcgtt tcgcccacgg tgtacagacg ttatttttcg accatccgaa ctgcattgcc
    40201 tttagccgca gtggcactga cgaatatccc ggctgcgtgg tggtcatgtc gaacggggat
    40261 gatggcgaaa aaaccattca cctgggagag aattacggca ataaaacctg gcgtgatttt
    40321 cttgggaacc ggcaagagag tgtagtaacc gacgaaaacg gcgaagcgac cttcttttgc
    40381 aacggcggca gcgttagcgt gtgggttatc gaagaggtga tttaaattca tccccggcgg
    40441 caagccgggg agatttcatt acggcaatgg cgtcggtaac ggcgctttat ccagcgcagc
    40501 ggcacactct gccgtcgggc gatccacacg ctccatcgtc ataccatcgt actcaatggt
    40561 gttaccgtca cgctcaacct cgtacagctc acgttttacg gtaacgttag tgagatcatc
    40621 agacatcaat gtcaacttgc ccggcacagc aatcacccgc tgccactgac ggcaatccag
    40681 agtatcgccc tctttaatga caatcaggct gcctatcgct tccggactga ccagcgcacg
    40741 ttgcggccct ttggtctgcc agtagcccgc cagccagtcc ggcgcggggg ttttcacgac
    40801 attgttatag ttttcgactt cggcacagcc cgccagcaac atgagcgcgc ctgcaattgc
    40861 gagttttttc atcattcgtc ctgcatgaga agaaaagagg attgtggcat taaagccctg
    40921 atgtcgccag cctttccagg ttcagagaga aatttgatct aactattatt tttgtgaatt
    40981 attaccccga aaataaattt gcatggtgta t
//
LOCUS       DAMZFT010000028        40675 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.028, whole genome shotgun sequence.
ACCESSION   DAMZFT010000028 DAMZFT010000000
VERSION     DAMZFT010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 40675)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40675)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40675
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.028"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            215..1312
                     /gene="mltA"
                     /locus_tag="PN977_001620"
     CDS             215..1312
                     /gene="mltA"
                     /locus_tag="PN977_001620"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase A"
                     /protein_id="HDI6041662.1"
                     /translation="MKGRWVKYLLMGTVVAMLAACSSKPTDRGQQYKDGKFTQPFSLV
                     NQPDAVGAPINAGDFAEQINHIRNSSPRLYGNQSNVYNAVQEWLRAGGDTRNMRQFGI
                     DAWQMEGADNYGNVQFTGYYTPVIQARHTRQGEFQYPIYRMPPKRGRLPSRAEIYAGA
                     LSDKYILAYSNSLMDNFIMDVQGSGYIDFGDGSPLNFFSYAGKNGHAYRSIGKVLIDR
                     GEVKKEDMSMQAIRHWGETHSEAEVRELLEQNPSFVFFKPQSFAPVKGASAVPLVGRA
                     SVASDRSIIPPGTTLLAEVPLLDNNGKFNGQYELRLMVALDVGGAIKGQHFDIYQGIG
                     PEAGHRAGWYNHYGRVWVLKTAPGAGNVFSG"
     gene            1389..2195
                     /gene="tcdA"
                     /locus_tag="PN977_001621"
     CDS             1389..2195
                     /gene="tcdA"
                     /locus_tag="PN977_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cyclic N6-threonylcarbamoyladenosine(37)
                     synthase TcdA"
                     /protein_id="HDI6041663.1"
                     /translation="MSVVISDAWRQRFGGTARLYGEKALQLFADAHICVVGIGGVGSW
                     AAEALARTGIGSITLIDMDDVCVTNTNRQIHALRDNVGLAKAEVMAERIRQINPECRV
                     TVVDDFVTPDNVAQYMSVGYSYVIDAIDSVRPKAALIAYCRRNKIPLVTTGGAGGQID
                     PTQIQVTDLAKTIQDPLAAKLRERLKSDFGIVKNSKGKLGVDCVFSTEALVYPQSDGT
                     VCAMKATAEGPKRMDCASGFGAATMVTATFGFVAVSHALKKMMAKAARQG"
     gene            complement(2246..2689)
                     /gene="csdE"
                     /locus_tag="PN977_001622"
     CDS             complement(2246..2689)
                     /gene="csdE"
                     /locus_tag="PN977_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase sulfur acceptor subunit
                     CsdE"
                     /protein_id="HDI6041664.1"
                     /translation="MTNPQFAGHPFGTTVTAETLRNTFAPLSQWEDKYRQLIMLGKQL
                     PALPDELKAQAKEIAGCENRVWLGYTMAENGKMHFFGDSEGRIVRGLLAVLLTAVEGK
                     TAAELQAQSPLALFDELGLRAQLSASRSQGLNALSEAIIAAAKQV"
     gene            complement(2689..3894)
                     /gene="csdA"
                     /locus_tag="PN977_001623"
     CDS             complement(2689..3894)
                     /gene="csdA"
                     /locus_tag="PN977_001623"
                     /EC_number="3.13.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase CsdA"
                     /protein_id="HDI6041665.1"
                     /translation="MNVFNPAQFRAQFPALQDAGVYLDSAATALKPEAVVEATRQFYS
                     LSAGNVHRSQFAEAQRLTARYEAAREKVAQLLNAPDDKTIVWTRGTTESINMVAQCYA
                     RPRLQPGDEIIVSVAEHHANLVPWLMVAQQTGAKVVKLPLNAQRLPDVDLLPELITPR
                     SRILALGQMSNVTGGCPDLARAITFAHSAGMVVMVDGAQGAVHFPADVQQLDIDFYAF
                     SGHKLYGPTGIGVLYGKPELLEAMSPWLGGGKMVHEVSFDGFTTQSAPWKLEAGTPNV
                     AGVIGLSAALEWLTDYDINQAESWSRSLATLAEEALAKRPGFRSFRCQDSSLLAFDFA
                     GVHHSDMVTLLAEYGIALRAGQHCAQPLLAELGVTGTLRASFAPYNTKSDVDALVNAV
                     DRALELLVD"
     gene            4086..4313
                     /locus_tag="PN977_001624"
     CDS             4086..4313
                     /locus_tag="PN977_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgdI/YgdR family lipoprotein"
                     /protein_id="HDI6041666.1"
                     /translation="MKKTAAIISACMLTFALSACSGSNYVMHTNDGRTIVSDGKPQTD
                     NDTGMISYKDANGNKQQINRTDVKEMVELDQ"
     gene            4665..5582
                     /gene="gcvA"
                     /locus_tag="PN977_001625"
     CDS             4665..5582
                     /gene="gcvA"
                     /locus_tag="PN977_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     regulator GcvA"
                     /protein_id="HDI6041667.1"
                     /translation="MSKRLPPLNALRVFDAAARHLSFTRAAEELFVTQAAVSHQIKSL
                     EDFLGLKLFRRRNRSLLLTEEGQSYFLDIKEIFSQLTEATRKLQARSAKGALTVSLLP
                     SFAIHWLVPRLSSFNSAYPGIDVRIQAVDRQEDKLADDVDVAIFYGRGNWPGLRVEKL
                     YAEYLLPVCSPLLLTGEKPLKTPEDLAKHTLLHDASRRDWQTYTRQLGLNHINVQQGP
                     IFSHSAMVLQAAIHGQGVALANNVMAQSEIEAGRLVCPFNDVLVSKNAFYLVCHDSQA
                     ELGKIAAFRQWILAKAAAEQEKFRFRYEQ"
     gene            5601..5996
                     /locus_tag="PN977_001626"
     CDS             5601..5996
                     /locus_tag="PN977_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HDI6041668.1"
                     /translation="MTSRFMLIFAAISGFIFVALGAFGAHVLSKTMGAVEMGWIQTGL
                     EYQAFHTLAILGLAVAMQRRISIWFYWSSVFLALGTVLFSGSLYCLALSHLRLWAFVT
                     PVGGVSFLAGWALMLVGAIRLKRKGVSHE"
     gene            5989..7089
                     /gene="rlmM"
                     /locus_tag="PN977_001627"
     CDS             5989..7089
                     /gene="rlmM"
                     /locus_tag="PN977_001627"
                     /EC_number="2.1.1.186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010435097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytidine(2498)-2'-O)-methyltransferase
                     RlmM"
                     /protein_id="HDI6041669.1"
                     /translation="MNKVVLLCRPGFEKECAAEITDKAGQREIFGFARVKENAGYVIY
                     ECYQPDDGDKLIRELPFSSLIFARQWFVVGELLQHLPPEDRITPIVGMLQGVVEKGGE
                     LRVEVADTNESKELLKFCRKFTVPLRAALRDAGVLANYETPKRPVVHVFFIAPGCCYT
                     GYSYSNNNSPFYMGIPRLKFPADAPSRSTLKLEEAFHVFIPADEWDERLANGMWAVDL
                     GACPGGWTYQLVKRNMWVYSVDNGPMAQSLMDTGQVTWLREDGFKFRPTRSNISWMVC
                     DMVEKPAKVAALMAQWLVNGWCRETIFNLKLPMKKRYEEVSHNLAYIQAQLDEHGINA
                     QIQARQLYHDREEVTVHVRRIWAAVGGRRDER"
     gene            complement(7133..7864)
                     /gene="fucR"
                     /locus_tag="PN977_001628"
     CDS             complement(7133..7864)
                     /gene="fucR"
                     /locus_tag="PN977_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose operon activator"
                     /protein_id="HDI6041670.1"
                     /translation="MKAARQQAIVDLLLNHTSLTTEALSEQLKVSKETIRRDLNELQT
                     QGKILRNHGRAKYIHRQNQDSGDPFHIRLKSHYAHKADIAREALAWIEEGMVIALDAS
                     STCWYLARQLPDINIQVFTNSHPICHELGKRERIQLISSGGTLERKYGCYVNPSLISQ
                     LKSLEIDLFIFSCEGIDSSGALWDSNAINADYKSMLLKRAAQSLLLIDKSKFNRSGEA
                     RIGHLDEVTHIISDERQVAASLVTA"
     gene            complement(7922..8344)
                     /gene="fucU"
                     /locus_tag="PN977_001629"
     CDS             complement(7922..8344)
                     /gene="fucU"
                     /locus_tag="PN977_001629"
                     /EC_number="5.1.3.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose mutarotase"
                     /protein_id="HDI6041671.1"
                     /translation="MLKTISPLISPELLKVLAEMGHGDEIIFSDAHFPAHSMGPQVIR
                     ADGLLVSDLLQAIIPLFELDSYAPPLVMMAAVEGDTLDPEVERRYRNALSLQAPCPDI
                     IRINRFAFYERAQKAFAIVITGERAKYGNILLKKGVTP"
     gene            complement(8346..9764)
                     /gene="fucK"
                     /locus_tag="PN977_001630"
     CDS             complement(8346..9764)
                     /gene="fucK"
                     /locus_tag="PN977_001630"
                     /EC_number="2.7.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculokinase"
                     /protein_id="HDI6041672.1"
                     /translation="MKQEVILVLDCGATNVRAIAVNRQGKIVARASTPNASDIAMENN
                     TWHQWSLDAILQRFADCCRQINSELTDCHIRGIAVTTFGVDGALVDKQGNLLYPIISW
                     KCPRTAAVMDNIERLISAQQLQAISGVGAFSFNTLYKLVWLKENHPQLLERAHAWLFI
                     SSLINHRLTGEFTTDITMAGTSQMLDIQQRDFSPQILQATGIPRRLFPRLVEAGEQIG
                     TLQNSAAAMLGLPVGIPVISAGHDTQFALFGAGAEQNEPVLSSGTWEILMVRSAQVDT
                     SLLSQYAGSTCELDSQAGLYNPGMQWLASGVLEWVRKLFWTAETPWQMLIEEARLIAP
                     GADGVKMQCDLLSCQNAGWQGVTLNTTRGHFYRAALEGLTTQLQRNLQMLEKIGHFKA
                     SELLLVGGGSRNTLWNQIKANMLDIPVKVLDDAETTVAGAALFGWYGVGEFNSPEEAR
                     AQIHYQYRYFYPQTEPEFIEEV"
     gene            complement(9873..11648)
                     /gene="fucI"
                     /locus_tag="PN977_001631"
     CDS             complement(9873..11648)
                     /gene="fucI"
                     /locus_tag="PN977_001631"
                     /EC_number="5.3.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose isomerase"
                     /protein_id="HDI6041673.1"
                     /translation="MKKISLPKIGIRPVIDGRRMGVRESLEEQTMNMAKATAALLTEK
                     LRHACGAAVECVISDTCIAGMAEAAACEEKFSSQNVGLTITVTPCWCYGSETIDMDPT
                     RPKAIWGFNGTERPGAVYLAAALAAHSQKGIPAFSIYGHDVQDADDTSIPADVEEKLL
                     RFARAGLAVASMKGKSYLSLGGVSMGIAGSIVDHNFFESWLGMKVQAVDMTELRRRID
                     QKIYDEAELEMALAWADKNFRYGEDENNKQYQRNAEQSRAVLRESLLMAMCIRDMMQG
                     NSKLADIGRVEESLGYNAIAAGFQGQRHWTDQYPNGDTAEALLNSSFDWNGVREPFVV
                     ATENDSLNGVAMLMGHQLTGTAQVFADVRTYWSPEAIERVTGHKLDGLAEHGIIHLIN
                     SGSAALDGSCKQRDSEGNPTMKPHWEISQQEADACLAATEWCPAIHEYFRGGGYSSRF
                     LTEGGVPFTMTRVNIIKGLGPVLQIAEGWSVELPKDVHDILNKRTNSTWPTTWFAPRL
                     TGKGPFTDVYSVMANWGANHGVLTIGHVGADFITLASMLRIPVCMHNVEETKVYRPSA
                     WAAHGMDIEGQDYRACQNYGPLYKR"
     gene            complement(11681..12997)
                     /gene="fucP"
                     /locus_tag="PN977_001632"
     CDS             complement(11681..12997)
                     /gene="fucP"
                     /locus_tag="PN977_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose:H+ symporter permease"
                     /protein_id="HDI6041674.1"
                     /translation="MGNTSIQTQSYRAVDKDAGQSRSYIIPFALLCSLFFLWAVANNL
                     NDILLPQFQQAFTLTNFQAGLIQSAFYFGYFIIPIPAGILMKKLSYKAGIITGLFLYA
                     FGAALFWPAAEIMNYTLFLVGLFIIAAGLGCLETAANPFVTVLGPESSGHFRLNLAQT
                     FNSFGAIIAVVFGQSLILSNVPHQSQDVLDKMSPEQLSAYKHSLVLSVQTPYMIIVAI
                     VLLVALLIMLTKFPALQSDNHSDAKQGSFSASLSRLARIRHWRWAVLAQFCYVGAQTA
                     CWSYLIRYAVEEIPGMTAGFAANYLTGTMVCFFIGRFTGTWLISRFAPHKVLAAYALI
                     AMALCLISAFAGGHVGLIALTLCSAFMSIQYPTIFSLGIKNLGQDTKYGSSFIVMTII
                     GGGIVTPVMGFVSDAAGNIPTAELIPALCFAVIFIFARFRSQTATN"
     gene            13544..14191
                     /gene="fucA"
                     /locus_tag="PN977_001633"
     CDS             13544..14191
                     /gene="fucA"
                     /locus_tag="PN977_001633"
                     /EC_number="4.1.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculose-phosphate aldolase"
                     /protein_id="HDI6041675.1"
                     /translation="MERNKLARQIIDTCLEMTRLGLNQGTAGNVSVRYQDGMLITPTG
                     IPYEKLTESHIVFIDGNGKHEEGKLPSSEWRFHMAAYQSRPDANAVVHNHAVHCTAVS
                     ILNRPIPAIHYMIAAAGGNSIPCAPYATFGTRELSEHVALALKIRKATLLQHHGLIAC
                     EANLEKALWLAHEVEVLAQLYLTTLAITDPVPVLSDEEIAVVLEKFKTYGLRIEE"
     gene            14219..15367
                     /gene="fucO"
                     /locus_tag="PN977_001634"
     CDS             14219..15367
                     /gene="fucO"
                     /locus_tag="PN977_001634"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HDI6041676.1"
                     /translation="MANRMILNETAWFGRGAVGALTDEVKRRGYQKALIVTDKTLVQC
                     GVVAKVTDKMDAAGLAWAIYDGVVPNPTITVVKEGLGVFQNSGADYLIAIGGGSPQDT
                     CKAIGIISNNPEFADVRSLEGLSPTNKPSVPILAIPTTAGTAAEVTINYVITDEEKRR
                     KFVCVDPHDIPQVAFIDADMMDGMPPALKAATGVDALTHAIEGYITRGAWALTDALHI
                     KAIEIIAGALRGSVAGDKDAGEEMALGQYVAGMGFSNVGLGLVHGMAHPLGAFYNTPH
                     GVANAILLPHVMRYNADFTGEKYRDIARVMGLKVEGMSLEEARNAAVEAVFALNRDVG
                     IPPHLRDVGVRKEDIPALAQAALDDVCTGGNPREATLEDIVELYHTAW"
     gene            complement(15472..16227)
                     /gene="xni"
                     /locus_tag="PN977_001635"
     CDS             complement(15472..16227)
                     /gene="xni"
                     /locus_tag="PN977_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417278.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flap endonuclease Xni"
                     /protein_id="HDI6041677.1"
                     /translation="MAVHLLIVDALNLIRRIHAVQGSPCVETCQHALDQLIMHSQPTH
                     AVAVFDDENRSSGWRHQRLPDYKAGRPPMPEELHNEIPALRAAFEQRGVPCWSASGNE
                     ADDLAATLAVKVTQAGHQATIVSTDKGYCQLLSPTLRIRDYFQKRWLDAPFIDKEFGV
                     QPQQLPDYWGLAGISSSKVPGVAGIGPKSATQLLVEFQSLEGIYENLDAVAEKWRKKL
                     ETHKEMAFLCRDIARLQTDLHIDGNLQQLRLVR"
     gene            complement(16338..17705)
                     /gene="sdaB"
                     /locus_tag="PN977_001636"
     CDS             complement(16338..17705)
                     /gene="sdaB"
                     /locus_tag="PN977_001636"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase II"
                     /protein_id="HDI6041678.1"
                     /translation="MISVFDIFKIGIGPSSSHTVGPMKAGKQFTDDLIARDLLKDVTR
                     VVVDVYGSLSLTGKGHHTDIAIIMGLAGNLPDTVDIDSIPGFIQDVNTHGRLMLANGQ
                     HEVEFPVDQCMNFHADNLSLHENGMRITALAGDKVVYSQTYYSIGGGFIVDEEHFGQQ
                     DSAPVEVPYPYSSAADLQKHCQETGLSLSGLMMKNELALHSKEELEQHLANVWEVMRG
                     GIERGISTEGVLPGKLRVPRRAAALRRMLVSQDKTTTDPMAVVDWINMFALAVNEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFIREVNANSLARYLLVASAIGSLYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGASPAQVCIAAEIAMEHNLGLTCDPVAGQVQVPC
                     IERNAIAAVKAVNAARMALRRTSEPRVCLDKVIETMYETGKDMNAKYRETSRGGLAMK
                     IVACD"
     gene            complement(17763..19052)
                     /gene="sdaC"
                     /locus_tag="PN977_001637"
     CDS             complement(17763..19052)
                     /gene="sdaC"
                     /locus_tag="PN977_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease SdaC"
                     /protein_id="HDI6041679.1"
                     /translation="METTQTSTIASKDSRSAWRKTDTMWMLGLYGTAIGAGVLFLPIN
                     AGVGGMIPLIIMAILAFPMTFFAHRGLTRFVLSGKNPGEDITEVVEEHFGIGAGKLIT
                     LLYFFAIYPILLVYSVAITNTVESFMSHQLGMTPPPRAILSLILIVGMMTIVRFGEQM
                     IVKAMSILVFPFVGVLMLLALYLIPQWNGAALETLSLDTASATGNGLWMTLWLAIPVM
                     VFSFNHSPIISSFAVAKREEYGDMAEQKCSKILAFAHIMMVLTVMFFVFSCVLSLTPA
                     DLAAAKEQNISILSYLANHFNAPIIAWMAPIIAIIAITKSFLGHYLGAREGFNGMVIK
                     SLRGKGKSIEINKLNRITALFMLVTTWIVATLNPSILGMIETLGGPIIAMILFLMPMY
                     AIQKVPAMRKYSGHISNVFVVVMGLIAISAIFYSLFS"
     gene            complement(19608..20972)
                     /gene="ppnN"
                     /locus_tag="PN977_001638"
                     /gene_synonym="ygdH"
     CDS             complement(19608..20972)
                     /gene="ppnN"
                     /locus_tag="PN977_001638"
                     /gene_synonym="ygdH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide 5'-monophosphate nucleosidase"
                     /protein_id="HDI6041680.1"
                     /translation="MITHISPLGSMDMLSQLEVDMLKRTASSDLYQLFRNCSLAVLNS
                     GSLTDNSKELLSRFENFDINVLRRERGVKLELINPPEEAFVDGRIIRALQANLFAVLR
                     DILFVYGQIHNTVRFPNLNLDNSVHITNLVFSILRNARALHVGEAPNMVVCWGGHSIN
                     ENEYLYARRVGNQLGLRELNICTGCGPGAMEAPMKGAAVGHAQQRYKDSRFIGMTEPS
                     IIAAEPPNPLVNELIIMPDIEKRLEAFVRIAHGIIIFPGGVGTAEELLYLLGILMNPA
                     NKDQVLPLLLTGPKESADYFRVLDEFVVHTLGENARRHYRIIIDDAAEVARQMKKSMP
                     LVKENRRDTGDAYSFNWSMRIAPDLQMPFEPSHENMANLKLYPDQPVEVLAADLRRAF
                     SGIVAGNVKEVGIRAIEEFGPYKINGDKEIMRRMDDLLQGFVAQHRMKLPGSAYIPCY
                     EICT"
     gene            complement(21084..21932)
                     /gene="queF"
                     /locus_tag="PN977_001639"
     CDS             complement(21084..21932)
                     /gene="queF"
                     /locus_tag="PN977_001639"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HDI6041681.1"
                     /translation="MSSYANHQALAGLTLGKSTDYRDTYDASLLQGVPRSLNRDPLGL
                     KADNLPFHGTDIWTLYELSWLNAKGLPQVAVGHVELDYTSANLIESKSFKLYLNSFNQ
                     TRFNNWDEVRQTLERDLSTCAQGKVSVALYRLDELEGQPIGHFNGTCIDDQDITIDNY
                     EFTTDYLENATSGEKVVEETLVSHLLKSNCLITHQPDWGSIQIQYRGRQIDREKLLRY
                     LVSFRHHNEFHEQCVERIFNDLLRFCQPEKLSVYARYTRRGGLDINPWRSNSDFVPST
                     TRLVRQ"
     gene            22000..22545
                     /gene="syd"
                     /locus_tag="PN977_001640"
     CDS             22000..22545
                     /gene="syd"
                     /locus_tag="PN977_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SecY-interacting protein"
                     /protein_id="HDI6041682.1"
                     /translation="MDDLTAQALKDFTARYCDAWHEEHKSWPLSEELYGVPSPCIIST
                     TEDAVYWQPQPFTGEQNVNAVERAFDIVIQPTIHTFYTTQFAGDMHAQFGDIKLTLLQ
                     TWSEDDFRRVQENLIGHLVTQKRLKLPPTLFIATLEEELEVISVCNLSGEVCKETLGT
                     RKRTHLASNLAEFLNQLKPLL"
     gene            23167..23496
                     /locus_tag="PN977_001641"
     CDS             23167..23496
                     /locus_tag="PN977_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqcC family protein"
                     /protein_id="HDI6041683.1"
                     /translation="MTTHDRVRFQLQALEALLREHQHWRNDEPQPHQFNSTQPFFMDT
                     MEPLEWLQWVLIPRMHDLLENNQPLPGAFAVAPYYEMALATDHPQRALILAELEKLDA
                     LFADDAS"
     gene            23496..24278
                     /gene="truC"
                     /locus_tag="PN977_001642"
     CDS             23496..24278
                     /gene="truC"
                     /locus_tag="PN977_001642"
                     /EC_number="5.4.99.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(65) synthase TruC"
                     /protein_id="HDI6041684.1"
                     /translation="MLEILYQDEWLVAVNKPSGWLVHRSWLDRDEKVVVMQTVRDQIG
                     QHVFTAHRLDRPTSGVLLMGLSSEAGRLLAQQFEQHQIQKRYHAIVRGWLMEEAVLDY
                     PLVEELDKIADKFAREDKGPQPAVTHYRGLATVEMPVATGRYPTTRYGLVELEPKTGR
                     KHQLRRHLAHLRHPIIGDSKHGDLRQNRSGAEHFGLQRLMLHASQLSLTHPFTGEPLT
                     IHAGLDDTWMQALSQFGWRGLLPENERVEFSAPSGQDGEISS"
     gene            24296..24745
                     /locus_tag="PN977_001643"
     CDS             24296..24745
                     /locus_tag="PN977_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HDI6041685.1"
                     /translation="MAEIGIFVGTMYGNSLLVAEEAEAILTAQGHKATVFEDPELSDW
                     LPYQDKYVLVVTSTTGQGDLPDCIVPLFQGIKDTLGFQPNLRYGVIALGDSSYVNFCN
                     GGKQFDTLLQEQSAQRVGEMLLIDASENPEPETESNPWVEHWGTLLS"
     gene            25180..26532
                     /gene="gudP"
                     /locus_tag="PN977_001644"
     CDS             25180..26532
                     /gene="gudP"
                     /locus_tag="PN977_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GudP"
                     /protein_id="HDI6041686.1"
                     /translation="MSSLSQAASSVEKRTNARYWIVVMLFIVTSFNYGDRATLSIAGS
                     EMAKDIGLDPVGMGYVFSAFSWAYVIGQIPGGWLLDRFGSKRVYFWSIFIWSMFTLLQ
                     GFVDIFSGFGIIVALFTLRFLVGLAEAPSFPGNSRIVAAWFPAQERGTAVSIFNSAQY
                     FATVIFAPIMGWLTHEVGWSHVFFFMGGLGIVISFIWLKVIHEPNQHPGVNQKELEYI
                     AAGGALINMDQQNTKVKVPFSVKWGQIKQLLGSRMMIGVYIGQYCINALTYFFITWFP
                     VYLVQARGMSILKAGFVASVPAVCGFIGGVLGGIISDWLMRRTGSLNIARKTPIVMGM
                     LLSMVMVFCNYVNVEWMIIGFMALAFFGKGIGALGWAVMADTAPKEISGLSGGLFNMF
                     GNISGIVTPIAIGYIVGTTGSFNGALIYVGVHALIAVLSYLVLVGDIKRIELKPVAGQ
                     "
     gene            26534..27874
                     /locus_tag="PN977_001645"
     CDS             26534..27874
                     /locus_tag="PN977_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucarate dehydratase"
                     /protein_id="HDI6041687.1"
                     /translation="MATQSSPVITDMKVIPVAGHDSMLLNIGGAHNAYFTRNIVVLTD
                     NAGHTGIGEAPGGEVIYQTLVEAIPMVLGQEVARLNKVVQQVHKGNQAADFDTFGKGA
                     WTFELRVNAVAALEAALLDLLGKALNVPVCELLGPGKQRDAITVLGYLFYIGDRTKTD
                     LPYLENTPGNHEWYQLRHQKAMNSEAVVRLADASQDRYGFKDFKLKGGVLPGEQEIDT
                     VRALKKRFPDARITVDPNGAWLLDEAISLCKGLNDVLTYAEDPCGAEQGFSGREVMAE
                     FRRATGLPVATNMIATNWREMGHAVMLNAVDIPLADPHFWTLSGAVRVAQLCDDWGLT
                     WGCHSNNHFDISLAMFTHVGAAAPGNPTAIDTHWIWQEGDCRLTKNPLEIKNGKIAVP
                     DAPGLGVELNWEQVQKAHEAYKRLPGGARNDAGPMQYLIPGWTFDRKRPVFGRH"
     gene            27895..29235
                     /gene="gudD"
                     /locus_tag="PN977_001646"
     CDS             27895..29235
                     /gene="gudD"
                     /locus_tag="PN977_001646"
                     /EC_number="4.2.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucarate dehydratase"
                     /protein_id="HDI6041688.1"
                     /translation="MSSQFSTPVVTEMQVIPVAGHDSMLMNLSGAHAPFFTRNIVIIK
                     DNSGHTGVGEIPGGEKIRKTLEDAIPLVVGKTLGEYKNVLTLVRNTFADRDAGGRGLQ
                     TFDLRTTIHVVTGIEAAMLDLLGQHLGVNVASLLGDGQQRSEVEMLGYLFFVGNRKAT
                     PLPYQSQPDDQCDWYRLRHEEAMTPDAVVRLAEAAYEKYGFNDFKLKGGVLAGEEEAE
                     SIVALAQRFPQARITLDPNGAWSLNEAIKIGTYLKGSLAYAEDPCGAEQGFSGREVMA
                     EFRRATGLPTATNMIATDWRQMGHTLSLQSVDIPLADPHFWTMQGSVRVAQMCHEFGL
                     TWGSHSNNHFDISLAMFTHVAAAAPGKITAIDTHWIWQEGNQRLTKEPFEIKGGLVQV
                     PQKPGLGVEIDMDQVMKAHELYQKHGLGARDDAMGMQYLIPGWTFDNKRPCMVR"
     gene            complement(29466..32222)
                     /gene="barA"
                     /locus_tag="PN977_001647"
     CDS             complement(29466..32222)
                     /gene="barA"
                     /locus_tag="PN977_001647"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase BarA"
                     /protein_id="HDI6041689.1"
                     /translation="MTNYSLRARMMILILAPTVLIGLLLSIFFVVHRYNDLQRQLEDA
                     GASIIEPLAVSTEYGMSLQNRESIGQLISVLHRRHSDIVRAISVYDENNRLFVTSNFH
                     LDPSSMQLGSNVPFPRQLTVTRDGDIMILRTPIISESYSPDESPSSDAKNSQNMLGYI
                     ALELDLKSVRLQQYKEIFISSVMMLFCIGIALIFGWRLMRDVTGPIRNMVNTVDRIRR
                     GQLDSRVEGFMLGELDMLKNGINSMAMSLAAYHEEMQHNIDQATSDLRETLEQMEIQN
                     VELDLAKKRAQEAARIKSEFLANMSHELRTPLNGVIGFTRLTLKTELTPTQRDHLNTI
                     ERSANNLLAIINDVLDFSKLEAGKLILESIPFPLRSTLDEVVTLLAHSSHDKGLELTL
                     NIKSDVPDNVIGDPLRLQQIITNLVGNAIKFTENGNIDILVEKRALSNTKVQIEVQIR
                     DTGIGIPERDQSRLFQAFRQADASISRRHGGTGLGLVITQKLVNEMGGDISFHSQPNR
                     GSTFWFHINLDLNPNIIIEGPSTQCLAGKRLAYVEPNSAAAQCTLDILSETPLEVVYS
                     PTFSALPPAHYDMMLLGIAVTFREPLTMQHERLAKAVSMTDFLMLALPCHAQVNAEKL
                     KQDGIGACLLKPLTPTRLLPALTEFCHHKQNTLLPVTDESKLAMTVMAVDDNPANLKL
                     IGALLEDMVQHVELCDSGHQAVERAKQMPFDLILMDIQMPDMDGIRACELIHQLPHQQ
                     QTPVIAVTAHAMAGQKEKLLGAGMSDYLAKPIEEERLHNLLLRYKPGSGISSRVVTPE
                     VNEIVVNPNATLDWQLALRQAAGKTDLARDMLQMLLDFLPEVRNKVEEQLVGENPEGL
                     VDLIHKLHGSCGYSGVPRMKNLCQLIEQQLRSGTKEEDLEPELLELLDEMDNVAREAS
                     KILG"
     gene            32279..33580
                     /gene="rlmD"
                     /locus_tag="PN977_001648"
     CDS             32279..33580
                     /gene="rlmD"
                     /locus_tag="PN977_001648"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HDI6041690.1"
                     /translation="MAQFYSAKRRTTTRQIITVSVNDLDSFGQGVARHNGKTLFIPGL
                     LPQENAEVTVTEDKKQYARAKVVRRLSDSPERETPRCPHFGVCGGCQQQHASEDLQQR
                     SKSAALARLMKHEVSEVIADVPWGYRRRARLSLNYLPKTQQLQMGFRKAGSSDIVDVK
                     QCPILVPQLEALLPKVRACLGSLQAMRHLGHVELVQATSGTLMILRHTAPLSSVDREK
                     LERFSHSEGLDLYLAPDSEILETVSGEMPWYDSNGLRLTFSPRDFIQVNAGVNQKMVA
                     RALEWLDVQPEDRVLDLFCGMGNFTLPLATQAASVVGVEGVPALVEKGQQNARLNGLH
                     NVTFYHENLEEDVTKQPWAKNGFDKVLLDPARAGAAGVMQQIIKLEPIRIVYVSCNPA
                     TLARDSEALLKAGYTIARLAMLDMFPHTGHLESMVLFSRVK"
     gene            33628..35862
                     /gene="relA"
                     /locus_tag="PN977_001649"
     CDS             33628..35862
                     /gene="relA"
                     /locus_tag="PN977_001649"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP diphosphokinase"
                     /protein_id="HDI6041691.1"
                     /translation="MVAVRSAHINKAGEFDPEKWIASLGITSQKSCECLAETWAYCLQ
                     HTQGHPDASLLLWRGVEMVEILSTLSMDIDTLRAALLFPLADANVVSEDVLRESVGKS
                     VVNLIHGVRDMAAIRQLKATHTDSVSSEQVDNVRRMLLAMVDDFRCVVIKLAERIAHL
                     REVKDAPEDERVLAAKECTNIYAPLANRLGIGQLKWELEDYCFRYLHPTEYKRIAKLL
                     HERRLDREHYIEEFVGHLRAEMKAEGVKAEVYGRPKHIYSIWRKMQKKNLAFDELFDV
                     RAVRIVAERLQDCYAALGIVHTHYRHLPDEFDDYVANPKPNGYQSIHTVVLGPGGKTV
                     EIQIRTKQMHEDAELGVAAHWKYKEGAAAGGARSGHEDRIAWLRKLIAWQEEMADSGE
                     MLDEVRSQVFDDRVYVFTPKGDVVDLPAGSTPLDFAYHIHSDVGHRCIGAKIGGRIVP
                     FTYQLQMGDQIEIITQKQPNPSRDWLNPNLGYVTTSRGRSKIHAWFRKQDRDKNILAG
                     RQILDDELEHLGISLKEAEKHLLPRYNFNDVDELLAAIGGGDIRLNQMVNFLQSQFNK
                     PSAEEQDAAALKQLQQKSYTPQNRSKDNGRVVVEGVGNLMHHIARCCQPIPGDEIVGF
                     ITQGRGISVHRADCEQLAELRSHAPERIVDAVWGESYSAGYSLVVRVVANDRSGLLRD
                     ITTILANEKVNVLGVASRSDTKQQLATIDMTIEIYNLQVLGRVLGKLNQVPDVIDARR
                     LHGS"
     gene            35940..36188
                     /gene="mazE"
                     /locus_tag="PN977_001650"
     CDS             35940..36188
                     /gene="mazE"
                     /locus_tag="PN977_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin MazE"
                     /protein_id="HDI6041692.1"
                     /translation="MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLII
                     EPVRKEPVFTLAELVNDITPENLHENIDWGEPKDKEVW"
     gene            36188..36523
                     /gene="mazF"
                     /locus_tag="PN977_001651"
     CDS             36188..36523
                     /gene="mazF"
                     /locus_tag="PN977_001651"
                     /EC_number="3.1.27.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endoribonuclease MazF"
                     /protein_id="HDI6041693.1"
                     /translation="MVSRYVPDTGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTG
                     MCLCVPCTTQSKGYPFEVVLSGQERDGVALADQVKSIAWRARGATKKGTVAPEELQLI
                     KAKINVLIG"
     gene            36595..37386
                     /gene="mazG"
                     /locus_tag="PN977_001652"
     CDS             36595..37386
                     /gene="mazG"
                     /locus_tag="PN977_001652"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HDI6041694.1"
                     /translation="MNQIDRLLTIMQRLRDPENGCPWDKEQTFATIAPYTLEETYEVL
                     DAIAREDFDDLRGELGDLLFQVVFYAQMAQEEGRFDFNDICAAISDKLERRHPHVFAD
                     SSAENSSEVLARWEQIKTEERAQKAQHSALDDIPRSLPALMRAQKIQKRCANVGFDWT
                     TLGPVVDKVYEEIDEVMYEAQQAVVDQAKLEEEMGDLLFATVNLARHLGTKAEIALQK
                     ANEKFERRFREVERIVAARGLEMTGVDLETMEEVWQQVKRQEIDL"
     gene            37614..39251
                     /gene="pyrG"
                     /locus_tag="PN977_001653"
     CDS             37614..39251
                     /gene="pyrG"
                     /locus_tag="PN977_001653"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase (glutamine hydrolyzing)"
                     /protein_id="HDI6041695.1"
                     /translation="MTTNYIFVTGGVVSSLGKGIAAASLAAILEARGLNVTIMKLDPY
                     INVDPGTMSPIQHGEVFVTEDGAETDLDLGHYERFIRTKMSRRNNFTTGRIYSDVLRK
                     ERRGDYLGATVQVIPHITNAIKERVLEGGEGHDVVLVEIGGTVGDIESLPFLEAIRQM
                     AVEIGREHTLFMHLTLVPYMAASGEVKTKPTQHSVKELLSIGIQPDILICRSDRAVPA
                     NERAKIALFCNVPEKAVISLKDVDSIYKIPGLLKSQGLDDYICKRFSLNCPEANLSEW
                     EQVIFEEANPVSEVTIGMVGKYIELPDAYKSVIEALKHGGLKNRVSVNIKLIDSQDVE
                     TRGVEILKGLDAILVPGGFGYRGVEGMITTARFARENNIPYLGICLGMQVALIDYARH
                     VANMENANSTEFVPDCKYPVVALITEWRDENGNVEVRSEKSDLGGTMRLGAQQCQLVD
                     DSLVRQLYNAPTIVERHRHRYEVNNMLLKQIEDAGLRVAGRSGDDQLVEIIEVPNHPW
                     FVACQFHPEFTSTPRDGHPLFAGFVKAASEFQKRQAK"
     gene            39338..40636
                     /gene="eno"
                     /locus_tag="PN977_001654"
     CDS             39338..40636
                     /gene="eno"
                     /locus_tag="PN977_001654"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HDI6041696.1"
                     /translation="MSKIVKIIGREIIDSRGNPTVEAEVHLEGGFVGMAAAPSGASTG
                     SREALELRDGDKSRFLGKGVTKAVAAVNGPIAQALIGKDAKDQAGIDKIMIDLDGTEN
                     KSKFGANAILAVSLANAKAAAAAKGMPLYEHIAELNGTPGKYSMPVPMMNIINGGEHA
                     DNNVDIQEFMIQPVGAKTVKEAIRMGSEVFHHLAKVLKAKGMNTAVGDEGGYAPNLGS
                     NAEALAVIAEAVKAAGYELGKDITLAMDCAASEFYKDGKYVLAGEGNKAFTSEEFTHF
                     LEELTKQYPIVSIEDGLDESDWDGFAYQTKVLGDKIQLVGDDLFVTNTKILKEGIEKG
                     IANSILIKFNQIGSLTETLAAIKMAKDAGYTAVISHRSGETEDATIADLAVGTAAGQI
                     KTGSMSRSDRVAKYNQLIRIEEALGEKAPYNGRKEIKGQA"
BASE COUNT        10058 a         9866 c        10842 g         9909 t
ORIGIN      
        1 cccgcgtccg tggatgcgca ctatactctg ctcgcgtttt catgcaacct tttttttacc
       61 ccgatcacgg atttggcatg attttgaaca aaaacaaatc actaaagcct attttttgtg
      121 caaaatttgc gtcggggaga ggctgctcat ttcattaaac gcggcggttt gttatcttcg
      181 ttgcgcctta ttttttaacc tgaagaagag aacaatgaaa ggacgttggg taaagtacct
      241 tcttatgggc acggttgtgg caatgcttgc cgcctgctct tccaaaccaa ccgatcgcgg
      301 acagcaatat aaagacggga aatttaccca gcctttctct ctggtgaacc agccagatgc
      361 cgttggcgcg ccgattaacg ccggtgattt tgccgagcaa attaaccata tccgtaattc
      421 gtcaccgcgt ctgtatggca accagagtaa tgtttataac gcggtgcaag agtggctgcg
      481 cgcaggcggt gatacccgca atatgcgcca gttcggcatt gatgcctggc agatggaagg
      541 tgccgacaac tatggtaacg tgcagtttac cggttattac acgccagtaa ttcaggcgcg
      601 ccatacccgc cagggcgagt ttcagtatcc gatttaccgt atgccgccaa aacgtggtcg
      661 tctgccgtct cgtgcggaga tctacgcggg ggcattgagc gataaataca tcctcgctta
      721 cagcaactct ctgatggata acttcattat ggatgtgcag ggtagtgggt atatcgactt
      781 tggtgatggc agtccgctta actttttcag ctatgcaggg aaaaacggtc atgcctatcg
      841 cagcattggt aaggtgctga tcgaccgtgg cgaagtgaaa aaagaagata tgtcgatgca
      901 ggcgattcgt cactggggtg aaacacacag tgaagccgag gttcgcgagc tgctggaaca
      961 gaacccgtct ttcgtcttct ttaaaccgca atcttttgct ccggtgaaag gggcaagtgc
     1021 ggtgccgctg gttggtcgcg cttcagttgc gtccgatcgt tccattatcc cgcctggtac
     1081 aaccttactg gcagaagtgc cgctgctgga taataacggc aaatttaacg gtcagtacga
     1141 actacggctg atggtggcgt tggatgtcgg tggtgcaatc aaaggccaac acttcgatat
     1201 ttatcaaggt atcgggccgg aagccggaca ccgcgcaggt tggtacaacc actatggacg
     1261 tgtttgggtg ctgaaaaccg ccccgggcgc aggtaacgtc tttagcggct gatgtgttat
     1321 tctgtatacc gttttgacgg atacgacagg gtgagggata acctcgccct ttttaattct
     1381 gaggtgctat gtctgtggta attagtgatg catggcgtca gcgttttggt ggcacagcgc
     1441 gtctgtatgg tgaaaaagca ttgcaactgt ttgctgacgc gcatatttgt gtggttggta
     1501 tcggtggtgt cggttcctgg gcggcggaag cgctggcgcg cacggggatt ggctcaatca
     1561 cgcttatcga tatggatgat gtgtgcgtca ccaataccaa tcggcaaatt catgccctgc
     1621 gcgataacgt tgggctggca aaagcggaag ttatggcgga gcgtattcgc cagattaacc
     1681 cggagtgccg tgtaacggtg gtggatgatt tcgtgacgcc ggataacgta gcgcagtata
     1741 tgagcgtggg ttattcgtac gtgattgatg ccattgatag tgtacggccc aaagcggcgc
     1801 tgattgctta ttgtcggcgc aataaaatcc cactggtcac aaccggtggc gcgggtgggc
     1861 agattgaccc gacgcagatt caggttaccg atctggcgaa aacgattcag gacccgctgg
     1921 cggcgaagtt gcgtgagcgc ctgaaaagcg attttggcat agtgaaaaac agtaaaggta
     1981 agctcggcgt ggattgcgtg ttctctaccg aagcgctggt gtacccgcag tcagatggca
     2041 cggtctgtgc aatgaaagct acggcggaag ggccaaagcg gatggattgt gcatctggat
     2101 ttggtgccgc gacgatggtg accgccacct ttggttttgt tgcggtttct catgcgctga
     2161 agaagatgat ggcgaaagcg gcgcgtcagg ggtaaacaat caggtcggat gcgacgcaaa
     2221 cgtcctttcc gacccggttc tgtgcttaaa cctgcttcgc cgcagcgata atcgcctcgc
     2281 ttaacgcatt caacccctgg ctgcgtgagg cgctaagttg cgcacgcaat cccagctcat
     2341 caaacaatgc cagcggcgac tgtgcctgca attcggcggc ggttttgccc tcaacggcag
     2401 tcaacaacac cgcaagcagg ccgcgcacaa tgcgtccttc gctgtcgcca aagaaatgca
     2461 tcttgccgtt ttcagccatg gtatatccca gccagacgcg gttttcgcat ccggcaatct
     2521 ctttagcctg cgcttttaac tcgtctggca aagccggcag ctgtttcccc agcatgatca
     2581 actggcgata tttatcttcc cattgcgaca acggtgcgaa ggtattgcgt aacgtttctg
     2641 cggttacggt tgtgccgaac ggatgtccgg cgaattgcgg gttcgtcatt aatccaccaa
     2701 taattccagc gcgcggtcaa cggcattcac cagcgcatcc acatcactct ttgtattata
     2761 tggcgcaaaa gaggcgcgca gtgtgccggt tacgcctaat tctgccagta gcggctgagc
     2821 gcaatgctgc ccggcccgca gggcaatacc gtactccgcc agcagtgtca ccatatcgct
     2881 gtgatgaact ccagcaaaat caaaggccag caggctggaa tcctggcagc ggaatgaacg
     2941 aaagcctgga cgtttcgcca gcgcttcttc tgcaagcgtt gctaagctac ggctccagct
     3001 ttcggcctgg ttgatatcgt aatctgtcag ccattccagc gccgcgctta atcctatgac
     3061 gccagcgaca tttggcgttc ctgcttccag tttccacggc gcagattgag tcgtgaagcc
     3121 gtcaaaactc acttcgtgaa ccattttgcc gccgcccagc cagggcgaca tcgcttccag
     3181 cagttctggt ttgccataca gcacgccgat gcctgtcggg ccatacagtt tgtgacctga
     3241 aaaagcatag aaatcaatat ccagttgctg aacatccgcg gggaaatgca ccgccccctg
     3301 agcaccatca accatcacca ccatcccggc tgaatgagca aaagtaatcg ctctcgccag
     3361 atccgggcaa ccgccagtga cgttcgacat ctggcccaac gccagaatcc gactacgggg
     3421 agtaatcagt tctggcaaca aatcgacatc cggcagtcgc tgcgcattaa gcggcaattt
     3481 caccactttg gcccccgttt gttgggcgac catcagccag gggacgaggt tggcgtggtg
     3541 ttctgccaca ctgacaataa tttcatcgcc cggttgcaga cgcggacgcg catagcattg
     3601 tgccaccatg ttgatggatt cagtggtgcc gcgcgtccag acgatagttt tatcatccgg
     3661 tgcattcagt aattgtgcca ctttttcccg cgcagcttcg taacgcgcgg tcaggcgttg
     3721 ggcttcggca aactggctgc gatggacgtt tccggcgctc aggctataaa actgtcgggt
     3781 ggcttcaacc acggcttcag gtttaagcgc ggtcgcggcg ctgtcgagat agacgcccgc
     3841 atcctgtagc gcgggaaact gggcgcgaaa ctgcgcggga ttaaaaacgt tcatggtact
     3901 cctcggcttg atgcaccgat cgtgacgcaa aattcacttt gatacaagga gagtgctaac
     3961 catcagaatt atctggatat cctggcgaag ccgcgtcagt aacatatgct gaatagtgtt
     4021 aggcgttaat tttagcctgt taatcaatac tattaagatt tattaatcgc tttaaggaga
     4081 agatgatgaa aaagactgcc gcaattattt ctgcctgtat gctgactttt gccctgagcg
     4141 cctgttccgg ttcgaactat gtgatgcaca ccaatgacgg acgtaccatc gtctctgacg
     4201 gcaaaccaca gactgataac gataccggta tgatttcgta taaagacgct aatggcaaca
     4261 aacagcagat caaccgtact gacgtgaaag agatggtcga actggatcag taattcgcga
     4321 tcgcaaggta aaaaaaaagc accgcaatta ggcggtgcta cattaatcac tatggacaga
     4381 cagggtaaat gtacaggaag tgaaaaaagg tagctttgct accatggtct gaatcgcaga
     4441 ccaattgcaa acacaacaac acaacatcac aaccgtaagc caaaagttca ccagaacacg
     4501 cattccgata aaacttttcg ttccggctca ggaagtgccg ccactatagg tatttgctgg
     4561 tagaagctca acggacaatt tataatggct cagattaaaa aaactaatag gttacacagt
     4621 gtgatctaat tgttaaattc atttaacatc aaagtttaaa agccatgtct aaacgattac
     4681 caccgctaaa tgccttacga gtttttgatg ccgcagcacg ccatttaagt ttcactcgcg
     4741 cagcagaaga gctttttgtg acccaggccg cagtaagtca tcaaatcaag tctcttgagg
     4801 attttttggg gctaaaactg ttccgccgcc gtaatcgttc actcctgctg accgaggaag
     4861 ggcaaagcta tttcctcgat atcaaagaga tattttcgca attaaccgaa gcgacgcgta
     4921 aactccaggc ccgtagcgct aagggggcgt tgacggtcag tttactcccc agtttcgcca
     4981 ttcattggtt ggttccgcga ctttccagct ttaattcagc ttatccggga attgacgttc
     5041 gaatccaggc ggttgatcgt caggaagata agctggcgga tgatgttgat gtggcgatat
     5101 tttatggtcg gggcaactgg ccggggctac gggtggaaaa actgtacgcc gaatatttat
     5161 tgccggtgtg ctcgccgcta ctgctgactg gcgaaaaacc cttgaagacc ccggaagatc
     5221 tggctaaaca tacgttatta catgatgcgt cacgccgtga ctggcagaca tatacccgac
     5281 agttggggtt aaatcatatc aacgttcagc aagggccaat ttttagccat agcgccatgg
     5341 tgctgcaagc ggctatccac gggcagggag tggcgctggc aaataacgtg atggcgcaat
     5401 ctgaaattga agccggacgt cttgtttgcc cgtttaatga tgttctggtc agtaaaaacg
     5461 ctttttatct ggtttgtcat gacagccagg cagaactggg taaaatagcc gcctttcgcc
     5521 aatggatcct ggcgaaagcc gctgctgaac aagaaaaatt ccgctttcgt tatgaacaat
     5581 aatttacgta gggtacgacc atgaccagcc gttttatgct gattttcgcc gccattagcg
     5641 gcttcatttt tgtggctctg ggcgcttttg gcgcgcatgt gttaagtaaa accatggggg
     5701 ccgttgagat gggctggatc cagaccggcc tcgaatacca ggcgtttcat acgctggcga
     5761 tcttaggtct ggcggtggca atgcagcgtc gcatcagtat ctggttttac tggagtagcg
     5821 ttttcctcgc gttaggcacg gtgctgttca gcggcagcct ttattgcctg gcgctgtccc
     5881 atctgcgttt gtgggcgttt gtcacaccgg ttggcggtgt gagcttcctt gcgggctggg
     5941 cgttaatgtt agttggtgct atccgtttaa agcgcaaggg cgtaagtcat gaataaggtt
     6001 gtattgctgt gccgtccggg ctttgaaaaa gagtgcgccg cagaaattac cgataaagcc
     6061 ggccagcggg aaattttcgg ttttgcccgc gtgaaagaga atgcgggtta tgtcatttat
     6121 gaatgttatc aacctgatga tggcgataag ttaatccgtg agctgccgtt cagttcatta
     6181 atttttgccc gccagtggtt tgtggtgggg gaactcctgc agcatttgcc gcctgaagat
     6241 cgtattaccc ccattgtcgg tatgttacag ggcgtagtag agaagggcgg tgaactgcgt
     6301 gttgaagttg ccgataccaa cgaaagcaaa gagttgctga aattctgccg taaattcacc
     6361 gtgccgctac gcgctgcctt gcgtgatgcg ggtgtgctgg cgaactatga aacgccgaag
     6421 cgtccggttg tgcatgtatt ctttattgca ccaggctgtt gctataccgg ttactcatac
     6481 agcaacaata attcgccgtt ctatatgggc attccgcgcc tgaaatttcc ggcagatgcg
     6541 ccgagtcgtt ccacgctcaa actggaagag gcatttcatg tgtttattcc cgcggatgag
     6601 tgggatgaac gcctggcgaa cgggatgtgg gcggtggatt tgggcgcttg cccaggcggc
     6661 tggacctacc aactggtgaa gcgcaacatg tgggtttatt ccgtcgacaa cggcccgatg
     6721 gcgcaaagtc tgatggatac cgggcaggta acgtggctgc gggaagacgg tttcaaattc
     6781 cgtccgacac gcagcaatat ctcctggatg gtatgcgata tggttgaaaa accggcgaaa
     6841 gttgcggcat tgatggcgca gtggctggtt aatggctggt gccgtgaaac cattttcaac
     6901 ctcaaactgc cgatgaaaaa acgctacgaa gaagtgtcac acaatctggc gtatattcag
     6961 gcacagcttg atgaacatgg cataaatgct cagatccagg cgcggcagtt gtatcacgat
     7021 cgcgaagaag tgacggtgca cgtccgccgt atctgggctg cggtgggtgg tcgtcgcgac
     7081 gagcgataac aggcaaggcc ggggcgtcgt ctccggcctg ctaccctttc cgtcaggctg
     7141 ttaccaaaga agccgcaacc tggcgctcat cagaaataat gtgcgtgacc tcatccagat
     7201 gcccgatgcg ggcttcccct gaacgattaa atttactttt atcaatcaat aacaacgatt
     7261 gcgcggcgcg ttttaatagc attgatttgt aatcagcgtt gatggcattg gagtcccaca
     7321 gtgcgccgct gctatcgatc ccttcacaag aaaaaataaa cagatcgatt tccagcgatt
     7381 taagctggga aatcagcgag ggattgacgt aacaaccata tttgcgctca agtgtgccgc
     7441 cggaactgat cagttgaatg cgttcacgtt taccgagttc atggcaaatc ggatggctat
     7501 tggtgaagac ctggatgttg atgtcaggca actggcgtgc cagataccag caggttgaac
     7561 tggcgtctaa tgctattacc atcccttctt caatccacgc gagcgcctcg cgcgcgatat
     7621 ctgctttatg cgcataatgg cttttcaacc ggatgtgaaa gggatcgcca ctgtcttgat
     7681 tttgacggtg gatatattta gcgcgtccat gattgcgcag aattttaccc tgcgtctgta
     7741 attcattgag atcgcgacga atggtttctt tactgacctt tagctgttca gagagagctt
     7801 ccgtggtcag gctggtatgg ttcagcagca ggtcgactat cgcttgctgg cgtgccgctt
     7861 tcatattttt caccccatca ttcgataacc gcccgcgtca tgcgggcgta ccggtatgag
     7921 attacggtgt tacccctttt tttaaaagaa tattcccgta cttcgctcgt tcgcctgtga
     7981 taacgatcgc aaaggctttt tgcgcccgtt cataaaacgc aaaacgattg atgcggatga
     8041 tgtcaggaca gggggcttgt agtgaaagcg cattacgata acgtcgttct acttcaggat
     8101 cgagagtgtc accttctacc gccgccatca tcaccagcgg cggtgcataa ctgtccagtt
     8161 caaataacgg gataatcgcc tggagcaagt cactcaccaa caggccatca gcgcggatca
     8221 cctgcggtcc catcgaatgg gcgggaaagt gagcatcgga aaaaataatt tcatctccat
     8281 gtcccatctc tgccagcact ttcaatagtt cgggagaaat taacggcgaa attgttttca
     8341 gcatttcaca cttcctctat aaattcaggt tcagtttgcg ggtagaaata acggtactga
     8401 taatgaatct gcgcgcgggc ttcttccggg ctgttaaatt cccctacgcc ataccaaccg
     8461 aacagcgcag ctcctgcgac ggtcgtttct gcgtcgtcga gaacttttac cggaatatca
     8521 agcatattgg ctttaatctg attccacaat gtgttgcgac ttcctccgcc aactaacaat
     8581 aattcagagg ctttaaagtg cccgattttt tccagcatct gtagattgcg ctgtaattgc
     8641 gtggttaacc cttccagcgc cgcgcgatag aaatgccccc gcgtggtatt aagcgtcact
     8701 ccttgccagc cagcgttctg acacgacaat aaatcacact gcattttcac tccatctgcg
     8761 ccaggagcga tcagacgagc ttcttcaatc aacatttgcc agggagtttc agccgtccag
     8821 aacagttttc tcacccattc cagcacgccg gatgccagcc attgcatacc tgggttatac
     8881 aaccctgcct ggctatccag ttcgcaggta gaaccggcgt actgacttaa cagcgaagta
     8941 tcaacctggg cgctgcggac cattaaaatt tcccatgtac cggaagagag cacgggttca
     9001 ttttgttcgg caccagcgcc aaaaagggcg aactgggtat cgtgacctgc ggaaatcacc
     9061 ggtatgccaa cgggtaagcc gagcattgct gcggcgctgt tctgtagcgt accaatctgt
     9121 tcacccgctt ccaccagacg agggaagagt cggcgtggaa taccggtggc ttgtaaaatt
     9181 tgcggactga aatcgcgttg ctggatatcc agcatctggc tggttccggc catcgtaata
     9241 tcagtagtga attcgccggt taaacggtgg ttaatcagcg acgaaataaa gagccaggcg
     9301 tgcgcgcgtt ccagcagttg tggatgattt tctttcaacc acaccaactt atataacgta
     9361 ttgaaactaa aggctccgac tccagaaata gcctgcaact gctgtgcgga gatcaaccgt
     9421 tcaatattgt ccattaccgc ggctgttcgc ggacatttcc agctaatgat cggatagagc
     9481 agattgcctt gcttatctac cagagcgcca tccacaccaa aggtggtgac ggcgataccg
     9541 cggatgtggc aatcagtcag ttcactattg atttgccgac agcaatcagc aaagcgttgc
     9601 aaaatggcgt ctaaagacca ctggtgccag gtgttgtttt ccatcgcgat atcgctggca
     9661 ttaggcgttg aggcgcgggc aacaattttt ccctgccgat taaccgcgat ggccctgaca
     9721 ttggtcgcgc cacagtcgag taccaggata acttcttgtt tcataatcgc tcctgcaata
     9781 tagccggata acattgctta tccggctaac cactcttgct tgttcaggcc cggtagccgg
     9841 agcgaccggg catcacatca gggagtaatg tattaacgct tgtacaacgg gccgtagttc
     9901 tggcaagcgc ggtaatcctg gccttcaata tccatgccgt gcgcggccca ggcagaagga
     9961 cgatacactt tggtctcttc aacgttgtgc atacataccg ggatacgcag catggaggca
    10021 agagtgataa agtctgcgcc aacgtggccg atggtcagaa ccccgtggtt agcgccccag
    10081 ttcgccatta ccgagtacac atccgtaaac ggccctttac cggtgaggcg cggtgcaaac
    10141 caggtggttg gccaggttga gttggtgcgt ttgttgagga tgtcatgcac atccttcggc
    10201 aattccacgc tccagccttc cgcgatttgc agtaccggtc ccaggccttt gatgatgttg
    10261 acacgagtca tggtgaacgg gacgccgcct tcggtaagga agcgggaaga gtaaccgccg
    10321 ccacggaagt attcgtggat cgccgggcac cattcggtag cggcgaggca agcgtcagcc
    10381 tcttgctgag agatttccca gtgcggcttc atcgtcgggt taccttcgct gtcgcgttgt
    10441 ttacaggaac cgtccagcgc agcagaaccg gagttgatca aatggatgat gccgtgttct
    10501 gccagtccat cgagtttatg ccccgttaca cgctcaattg cttctggtga ccagtaggta
    10561 cgcacatcgg caaatacctg agcggtgccg gtgagttggt gacccattag cattgccacg
    10621 ccgttaagac tgtcgttttc ggtcgccacg acaaagggtt cgcgcacgcc attccagtca
    10681 aatgaactgt tgaggagcgc ttcggcggtg tcaccattgg gatattgatc ggtccagtgg
    10741 cgttgtccct ggaagcccgc agcgatggcg ttgtagccaa gtgattcttc cacgcgacca
    10801 atatcggcca gtttgctgtt gccttgcatc atgtcgcgga tacacatcgc catcagtaaa
    10861 ctttcgcgca gaactgcgcg gctttgctcg gcattgcgtt gatactgttt gttattttca
    10921 tcttcgccat aacggaagtt tttatcagcc caggccagcg ccatttccag ttcggcttcg
    10981 tcgtaaatct tctgatcgat acggcgacgc agttcggtca tatccaccgc ctggactttc
    11041 attcccagcc aggattcaaa gaagttgtga tcaacaatag aaccggcgat acccatcgaa
    11101 acgccgccca gcgacagata gcttttacct ttcatgctgg cgacggccaa accggcgcgg
    11161 gcaaagcgca gcagtttttc ttcaacatca gcaggaatcg atgtgtcatc ggcatcctga
    11221 acgtcatgac cgtaaatgga gaatgccggg atgcctttct ggctgtgagc tgccagagcc
    11281 gctgccaggt aaacagcgcc gggacgttcg gtgccgttaa agccccaaat ggctttcggg
    11341 cgggttggat ccatgtcgat ggtttcactg ccatagcacc agcaaggggt taccgtaatg
    11401 gtgaggccta cattctgact gctgaatttt tcttcgcaag cagcggcttc agccataccc
    11461 gcgatacagg tatcggaaat gacacactcg acggcagctc cacaggcatg gcgcagtttc
    11521 tcggtcagca gtgcggccgt ggctttcgcc atattcattg tttgttcttc aagcgactca
    11581 cgaacaccca tgcgacgacc gtcaataacc gggcggatac caattttcgg taagctgatt
    11641 tttttcatta cagattcctc actttattcg gaaaatatgt tcagttagtt gccgtttgag
    11701 aacggaaacg ggcaaagata aagatgactg cgaagcagag tgcggggatc agttcagctg
    11761 tggggatgtt gcccgcagcg tcactgacaa aacccatgac cggagtgaca ataccgccgc
    11821 caataatggt cataacgatg aaggacgaac cgtatttggt gtcctggccg agattcttaa
    11881 tgcccagcga gaagattgtt gggtactgaa tcgacataaa ggcactgcat aaagtcaggg
    11941 ctattaaacc cacatgaccg ccagcgaagg ctgagatcag gcacagtgcc atagcaatta
    12001 atgcgtaggc tgccaggact ttgtgtggtg cgaagcgact gatgagccag gtaccggtga
    12061 aacgaccaat aaagaagcac accatggttc cggttaaata gttagcggca aagcctgctg
    12121 tcatacctgg aatttcttct acagcgtagc gaattaaata gctccagcag gccgtttgtg
    12181 cgccaacgta gcagaattgc gctaatactg cccagcgcca gtggcgaata cgcgccaggc
    12241 gagaaagcga tgcggagaac gatccttgtt tggcgtcact gtgattatca ctctgcaatg
    12301 ccgggaattt cgtcagcatg atcagcaggg cgaccagtaa cacgatagcc acgatgatca
    12361 tataaggtgt ctgtaccgat aataccaggc tgtgtttata cgcactcaat tgctctggag
    12421 acattttatc gagaacgtct tgcgattgat gtggcacgtt agacaaaata agactttgcc
    12481 caaagacaac cgcgataatt gcgccaaatg agttaaatgt ttgcgcaaga tttaagcgga
    12541 agtgaccact gctttccggc cctaataccg taacaaaagg gtttgcggca gtttccagac
    12601 aacctaatcc ggctgcaata ataaataggc caactaaaaa caaggtgtag ttcattattt
    12661 ctgcggcggg ccagaataat gcagcgccga aggcatataa aaacaatccg gtaataatcc
    12721 ctgctttata actgagtttt ttcatcaata tcccggcagg gattgggata atgaaataac
    12781 caaagtaaaa ggccgattgg atcaggccag cctggaaatt tgtcagcgta aaagcctgct
    12841 ggaattgagg caataaaatg tcgttaaggt tattggctac cgcccaaaga aaaaacagtg
    12901 agcacagcag tgcaaatgga ataatgtaac ttctgctttg ccctgcatct ttatctaccg
    12961 cacggtaact ctgcgtttgt attgatgtgt ttcccatagc atcctcttag gttcagaagc
    13021 ttaagcacgt aatcggttat gttttcaaaa gtgcaaaacc agcgagttcg ggcttcatat
    13081 cagtaaatat ttgcctgtct gaaatgaact catggattca ttttcaaata aaaaacgctc
    13141 tgtaatatga cggcggtcac acttattagc cggaaggtga ctttatgtga ctaccatcac
    13201 tttaataatc gaaatttcat gcccgtttca gaaaataaat cataaaaaac acaaaccaga
    13261 tgaccgtatt aattgtttgt gccgaaaacg ggcattcctg gcagggaaga gtagaaaaat
    13321 gaccgttttg ataaatattt ttaagagcgg tcatttttaa tggggttata aaaagagaaa
    13381 ttgcagttat ttactgtgat tagttgaacc aggtcacaaa atataagctt gtgaatttct
    13441 ttctggttaa tcaggaagaa tatggtgagt aattaaacgg ctaattcaat agtgtgaaag
    13501 gaacaacatt attgccctgt tttgaatcag agagaggtag ctaatggaac gaaataaact
    13561 tgctcgtcag attattgaca cttgcctgga aatgactcgc ctggggctga accaggggac
    13621 agcgggtaac gtcagtgtac gttatcagga tgggatgctg attacgccta caggtattcc
    13681 atatgaaaaa ctgacggagt cgcatattgt ctttattgat ggcaacggca aacatgagga
    13741 aggaaaactc ccctcaagcg agtggcgttt ccatatggcg gcctatcaaa gcagaccgga
    13801 tgccaacgcg gttgttcaca atcatgccgt tcattgtacg gcagtttcca ttcttaaccg
    13861 accgatcccc gctattcact acatgattgc ggcggctggc ggtaattcta ttccttgcgc
    13921 gccttatgcg acctttggaa cacgcgaact ttctgaacat gttgcgctgg ctctcaaaat
    13981 tcgtaaggca actttgttac aacatcatgg gcttatcgct tgtgaggcga atctggagaa
    14041 agcgttatgg ctggcgcatg aagttgaagt gctggcacaa ctttacctga cgaccctggc
    14101 gattacggac ccggtgccag tgctgagcga tgaagagatt gccgtagtgc tggagaaatt
    14161 caaaacctac gggttacgga ttgaagagta atttcgtaaa gcaacaagga gaaggatgat
    14221 ggctaacaga atgattctga acgaaacggc atggtttggt cggggtgctg ttggggcttt
    14281 aaccgatgag gtgaaacgcc gtggttatca gaaggcgctg atcgtcaccg ataaaacgct
    14341 ggtgcaatgc ggcgtggtgg cgaaagtgac cgataagatg gatgctgcag ggctggcatg
    14401 ggcgatttac gacggcgtag tgcccaaccc aacaattact gtcgtcaaag aagggctcgg
    14461 tgtattccag aatagcggcg cggattacct gatcgctatt ggtggcggtt ctccacagga
    14521 tacgtgtaaa gcgattggca ttatcagcaa caatccggag tttgccgatg tgcgtagcct
    14581 ggaagggctt tccccgacca ataaacccag tgtaccgatt ctggcaatcc ccaccacagc
    14641 aggtactgcg gcagaagtga ccattaacta cgtgatcact gacgaagaaa aacggcgcaa
    14701 gtttgtttgc gttgatccgc atgatatccc gcaggtggcg tttattgacg ctgacatgat
    14761 ggatggtatg cctccagcgc tgaaagccgc gacgggtgtc gatgcgctca ctcatgctat
    14821 tgaggggtat attacccgtg gcgcgtgggc gctaaccgat gcactgcaca ttaaagcgat
    14881 tgaaatcatt gctggggcgc tgcgaggatc ggttgctggt gataaggatg ccggagaaga
    14941 aatggcgctc gggcagtatg ttgcgggtat gggcttctcg aatgttgggt tagggttggt
    15001 gcatggtatg gcgcatccac tgggcgcgtt ttacaacacg ccacacggtg ttgcgaacgc
    15061 catcctgtta ccgcatgtca tgcgctataa cgctgacttt accggtgaga agtaccgcga
    15121 tatcgcgcgc gttatgggcc tgaaagtgga aggtatgagc ctggaagagg cgcgtaatgc
    15181 cgctgttgaa gcggtgtttg ctctcaaccg tgatgtcggt attccgccac atttacgtga
    15241 tgttggtgta cgcaaggaag acattccggc actggcgcag gcggcactgg atgatgtttg
    15301 taccggtggc aacccgcgtg aagcaacgct tgaggatatt gtagagcttt accataccgc
    15361 ctggtaaatg cgctgatgtg ataatgccgg atgcgacgct agccgtgtct tatccggcat
    15421 ataagtgcat taaattcaat gtgttgataa tcgtttgcat ccggcacgcc gttaccgtac
    15481 caaccgcaat tgctgtaaat tgccgtcgat atgcaaatcg gtttgtaagc gggcaatatc
    15541 gcggcacaga aacgccatct ctttatgggt ttctaatttt ttgcgccact tttcggcaac
    15601 cgcatccaga ttctcatata tcccttccag actctgaaac tcgaccagta gctgtgtggc
    15661 gctttttggt ccgatccccg caacacccgg tacctttgaa ctgctgatcc ccgcaagccc
    15721 ccagtaatcg ggcaactgct gcggttgaac gccaaattct ttatcgataa atggcgcatc
    15781 cagccaacgt ttctggaagt aatcacgaat acgtaatgtc ggtgaaagta actgacagta
    15841 gcctttatct gtcgaaacaa tcgttgcctg atgcccggcc tgtgtcactt tgaccgccag
    15901 cgtggcggct aagtcatcgg cttcgttgcc gctggcggac cagcacggga cgccgcgttg
    15961 ctcaaaggcg gcgcgtaatg caggtatctc gttgtgcaac tcttccggca ttggtggtcg
    16021 acccgctttg taatctggta aacgctgatg acgccagccg ctactgcggt tttcatcatc
    16081 aaaaacggcg accgcgtggg ttggctggct gtgcataatg agctgatcga gcgcgtgctg
    16141 gcaggtttcg acacagggcg aaccctgaac ggcatgaatg cgacgaataa gattcagtgc
    16201 atcgacaata agcaaatgaa cagccacgat aaccccctta aagatcaggc gataagggta
    16261 acattcgatg ccagaacagg ctatggctgg atgagaaaac gggaagaggc ctcgcaaaac
    16321 gaggcctccg ggagagatta atcgcaggca acgatcttca ttgccaggcc accgcgagag
    16381 gtttcgcggt acttggcgtt catatcttta cctgtttcgt acatggtctc gataacttta
    16441 tcgaggcaga cgcgcggctc gctggtacgg cgcagcgcca tacgtgcagc atttaccgct
    16501 ttcactgccg caatggcgtt acgctcgatg catggcacct ggacctgtcc agcaaccggg
    16561 tcacacgtca gaccgaggtt gtgctccatg gcgatttccg ccgcgatgca cacctgcgcc
    16621 gggcttgcgc ctaacagttc tgccagacca gccgccgcca ttgagcacgc cacgccaact
    16681 tcaccctggc aacccacttc ggcaccagaa atcgacgcgt tcatcttata aagagaacca
    16741 atggcgctgg ctaccagcag gtaacgagcc agtgagttag cgttcacttc gcggataaac
    16801 ttatcgtagt acgccagcac agctgggata atcccgcacg caccgttagt cggcgcagtc
    16861 accacgcgac cgccagcagc gttctcttcg ttcactgcca gtgcaaacat gttgatccag
    16921 tcaacaaccg ccatcgggtc agtggtggtt ttatcctggc tgaccagcat ccggcgtagt
    16981 gccgcagcac ggcgtggaac gcgcagtttg ccaggtaaca cgccttcggt agaaataccg
    17041 cgctcaatac cgccacgcat gacttcccag acgttcgcca ggtgctgttc cagctcttct
    17101 ttgctgtgca gcgccagctc gtttttcatc atcaggccag agagtgacag cccggtttcc
    17161 tgacaatgtt tttgcagatc ggctgctgaa ctgtacggat aaggaacttc aaccggtgcg
    17221 ctatcctgct gaccaaaatg ttcttcatca acaataaagc caccgccgat agagtaataa
    17281 gtctggctgt aaacgacttt atcgcccgcc agcgcggtaa tgcgcatacc gttttcatgc
    17341 agagaaaggt tgtcggcatg gaagttcatg cactgatcaa ccgggaactc cacttcatgc
    17401 tgaccgtttg ccagcatcag gcgaccatga gtattcacat cctgaataaa accagggatg
    17461 gaatcgatat ccacggtatc cggcaggtta cccgccagac ccataattat ggcgatatca
    17521 gtgtggtggc ctttaccggt cagagagagc gagccgtaca cgtcaaccac cacgcgggtc
    17581 acgtctttaa gcaggtcacg ggcaatcaga tcgtcggtaa attgtttacc cgctttcatt
    17641 ggtccaacgg tatgagaact ggaagggcca atgccgattt tgaaaatatc gaatacgcta
    17701 atcataggaa atacatcgcg ttaaaacgga ggaagcgccg cccgaaagcg gcgcgaaagg
    17761 acttagctga acagagagta gaagattgcg gagattgcaa tcagacccat cacgacaacg
    17821 aatacgttgc tgatgtgacc gctgtactta cgcattgccg gtactttctg aattgcgtac
    17881 atcggcatca ggaacaggat catcgcgatg attgggccgc ccagggtttc aatcataccc
    17941 aggatgctcg ggttcagggt ggcaacaatc caggtcgtta ccagcatgaa cagcgcagtg
    18001 atacggttca gcttgttgat ttcgatagac ttacctttac cacgcagaga tttaatcacc
    18061 ataccgttga agccttcacg tgcgcccagg tagtggccga ggaaggattt ggtgatagcg
    18121 ataatcgcga taatcggagc catccacgcg atgatcggcg cgttaaagtg gttagccagg
    18181 taagacagaa tcgagatgtt ctgctcttta gccgcagcca ggtctgccgg agtcaggctc
    18241 agtacgcagc tgaagacgaa gaacattacg gtcagcacca tcatgatgtg tgcgaatgcc
    18301 aggatcttag agcatttctg ttctgccata tcgccgtact cttcacgctt cgcaacggcg
    18361 aaagaagaga tgatcggaga gtggttgaac gagaacacca ttaccggaat tgccagccac
    18421 agggtcatcc acagaccgtt tccggttgca gatgcagtgt ccagagacag tgtttccagt
    18481 gctgcgccgt tccactgcgg gatcaggtac agagccagca gcatcagtac gccaacaaac
    18541 gggaatacca gaatgctcat cgctttaacg atcatctgct caccgaagcg aacgatggtc
    18601 atcataccca cgatcaggat cagcgacaga atcgcacgcg gcggtggtgt catacccagc
    18661 tggtgagaca tgaagctttc aactgtattg gtgattgcca cgctataaac cagcaggatc
    18721 gggtagatag cgaagaagta gagcagggta atcagtttac ctgcgccaat accaaagtgt
    18781 tcttctacaa cctcggtgat gtcttcgcct gggtttttac cagacagtac gaagcgagtc
    18841 aggccgcggt gagcaaaaaa tgtcatcggg aacgcaagga tagccatgat gatcagcggg
    18901 atcataccgc caacaccggc gttgattggc aggaacagca cgcccgcgcc gattgccgtg
    18961 ccgtaaaggc ccagcatcca catggtgtct gtcttgcgcc aggcactacg agagtctttc
    19021 gacgcaatcg tgctggtttg agtcgtttcc atctatttct cctggaggaa tgcaaataat
    19081 tcagctttta gccagttcag cccgggaagg tgcctgaccg taaaaatgaa atctgtctat
    19141 cggcgatggc ttaatcattt accgccgtaa taatttgagg gcggaaagat acacacaagt
    19201 tatgtatcta tcagtgatct tgatcccaaa tacaataatc tacttggaag atagattatc
    19261 ccaacgcttt actctgtaaa tcaaaagtaa aagagaattt aagggcaata tttgcccatt
    19321 aaacgacatc tgacttcttc cggggcgcgc aatatatgta ggttgaactg aaaaaatcaa
    19381 tacttttagt taatcatggc gataatttct ttttcatagc gataaaatgt ggtttatgaa
    19441 aatagggaaa cgtttgcgtt acaacataaa ttgtatgaca tattaactat aatgatattt
    19501 atcgcgtgag ggtggtaaga aacattcata taacaaatga aataaattga tgggtatgtg
    19561 accaaaaacg agaaaacccg gcaacaaacg tcaccggggg agggagatta cgtgcagatt
    19621 tcgtagcaag ggatgtaggc tgagcctggc aacttcatac gatgctgggc aacaaaaccc
    19681 tgtagcaggt catccatacg acgcataatc tctttatcgc cgttgatttt ataaggacca
    19741 aactcttcaa tggcgcgaat accgacttct tttacgttac ccgccacaat accggagaac
    19801 gcacggcgca ggtcggcagc cagcacttca acgggttgat ccgggtaaag cttcagatta
    19861 gccatattct cgtgagacgg ctcaaacggc atttgcaaat ctggcgcaat gcgcattgac
    19921 cagttaaagc tgtaggcatc gcctgtgtcg cggcgatttt ctttcaccag cggcatcgat
    19981 tttttcatct gacgagcgac ttcagcggcg tcatcaatga tgatgcggta atggcggcgc
    20041 gcgttttcac ccaacgtatg cacgacaaac tcgtccagta cgcggaagta gtcggcgctc
    20101 tctttcgggc cggtgaggag caatggtaaa acctgatctt tgttggccgg gttcattaaa
    20161 attcccagca aatacagcaa ctcttctgcc gtacccacac cgccagggaa gataatgata
    20221 ccgtgagcga tacggacaaa cgcttccaga cgtttttcga tatccggcat gatgatcaat
    20281 tcgttgacca gcgggttagg cggttcagcg gcgataatcg atggctctgt cataccaata
    20341 aaacgactgt ctttgtaacg ctgctgtgcg tgtccgaccg cagcaccttt cattggcgct
    20401 tccatcgctc ccggaccaca gccggtgcag atattcagct cacgcaggcc cagctgattc
    20461 ccgacgcgac gggcatacaa atactcgttt tcgttaattg agtgcccgcc ccagcagacc
    20521 accatatttg gcgcttcacc aacatgcagc gcgcgagcgt tacgcaagat ggaaaagacc
    20581 aggttagtga tgtggacgga gttgtcgaga ttcaggttgg gaaaacgaac ggtgttatgg
    20641 atttgcccgt aaacgaagag aatgtcgcgc agaaccgcga acaagttggc ctgcaaagcg
    20701 cgaataattc gcccatcgac aaaagcctct tccgggggat taatcagttc cagctttacg
    20761 ccgcgttcac ggcgcaggac gttaatatcg aaattttcaa aacgagacag caattctttg
    20821 ctgttatcgg tcaaactacc ggagttcagt acggcaagtg aacagttgcg aaacagttga
    20881 tagaggtcgc tgctggcggt gcgtttaagc atatccactt ccagctgcga caacatatcc
    20941 atggagccaa gcgggctaat atgtgtaatc aagaaaaact ccttatggga cgaatatcgt
    21001 cattcccttt gattacaata gccctgcctg atgacctttc acaaccttct gcgtgaatcc
    21061 agcacgcaaa attgagaaaa aatttattgc cgaaccagtc ttgtggtcga tgggacaaaa
    21121 tcgctattac tgcgccacgg gttaatgtcc agaccgccac gacgggtata acgtgcgtaa
    21181 acgctcaatt tctctggctg gcagaagcgt aacaggtcat taaagatgcg ttccacgcac
    21241 tgttcgtgga actcgttgtg atggcggaaa gagaccaggt aacgcagcag tttttctctg
    21301 tcaatttggc gtccacgata ctgaatttgg atcgatcccc aatctggttg atgggtgatc
    21361 aggcagtttg atttcagcag gtggctgacg agcgtctctt ctactacttt ttcgccactg
    21421 gtggcattct ccagatagtc agtagtgaat tcatagttat cgatagtgat atcctggtca
    21481 tcaatgcaag tgccattgaa atgacctatc ggttggcctt ccagttcatc aagacgatat
    21541 aacgccacgc taaccttacc ctgagcgcaa gtgcttaagt cgcgctccag cgtctggcgc
    21601 acctcatccc agttattaaa acgcgtctgg ttaaaactgt tgagatagag cttaaaactc
    21661 ttcgactcaa tcagatttgc gctggtgtaa tcaagttcaa catgaccgac ggcgacctgc
    21721 ggcaaacctt tcgcattcag ccaggaaagt tcatatagcg tccagatatc cgtaccgtga
    21781 aaaggcaggt tatccgcttt cagacccagc ggatcgcgat tcagactacg cggaacgcct
    21841 tgcagtaggc tggcgtcata ggtatcccgg taatcggttg attttccaag agtcaggccc
    21901 gcaagtgcct gatggtttgc ataagaagac atgtttcacc gtcataaacc agataaacta
    21961 aacgacaagt gtatctggtt taagtgaaga gagaaattag tggacgattt gaccgcacaa
    22021 gccctgaaag attttactgc acgctactgc gatgcgtggc atgaagagca taaaagctgg
    22081 ccgttgagtg aggaactgta cggtgttcct tcaccgtgca ttatttcaac taccgaagat
    22141 gccgtatact ggcaacccca gccgtttacc ggggaacaaa atgtaaacgc ggttgaacgt
    22201 gcttttgata ttgtgataca acccacaatt catacctttt atacgactca gtttgccggg
    22261 gatatgcacg cgcagtttgg cgatatcaaa ctgacattgc tgcaaacctg gagtgaagac
    22321 gacttccgtc gggtgcagga aaatctgatt ggtcatctgg tgactcagaa aaggctaaaa
    22381 ctgccgccca cgctatttat cgcgacgctg gaagaagagt tagaggtgat ctcggtgtgt
    22441 aatctctccg gtgaagtgtg caaagagacg ctgggaacac gtaaacggac gcatttagcc
    22501 tcaaatcttg cggaattcct taatcaactt aagcctcttc tgtaatccaa ataccccatc
    22561 tggttgtgag agatctctta cagactctgt aggagatcgc caggaaataa gcgaatactt
    22621 aaaaagataa gaatcgttat tttcatttaa aatcaatata ttgcttgtca attcttaact
    22681 ttcatatgaa attttcctta aggcattgtc tgtaagcgcc ttgtaagact tcgcgaaaaa
    22741 gacgattcta tcttcgtcga cagggagtca gacaacgaag tgaacatcag gatgatgaca
    22801 cttctgaagg acacaccagg atggtgtttc agggaaaggc ttctggatga agcgaagagg
    22861 atgacgcagg acgcgttaaa ggacacctcc aggatggaga atgagaaccg gtcaggatga
    22921 ttcggtgggt caggaaggcc aggaacactt caggatgaag tatcacatcg gggtggtgtg
    22981 agcaggaagc aatagttcag gatgaacgat tggtcgcaag gccagaggaa aagttgtcaa
    23041 ggatgagcag ggagcaacaa aagtagctgg aatgctgcga aacgaaccgg gagcactgtg
    23101 aatacagtgc tccctttttt tattcctgct atccttcgcg gcagtttttc ttattgaggt
    23161 tgctttatga ccactcatga ccgcgttcgt ttccagttgc aggcgcttga agcgttactg
    23221 cgtgaacatc agcactggcg aaacgatgaa ccccagccgc atcaatttaa tagtacccaa
    23281 ccgttcttta tggacaccat ggaaccactg gagtggttgc agtgggtgct gatcccgcga
    23341 atgcacgatt tgttagaaaa caaccagccg ttaccgggcg cttttgccgt tgctccctat
    23401 tatgaaatgg cgctggcgac ggaccacccg caacgcgcgc ttattctggc tgagctggaa
    23461 aaactggatg ctctttttgc ggatgatgcg agctaatgct ggaaatactc tatcaggatg
    23521 aatggctggt tgcggtaaat aaaccctccg gctggctggt tcaccgcagc tggctggatc
    23581 gcgacgagaa agtagtggtc atgcaaaccg tgcgtgacca gataggccag catgtgttta
    23641 ctgctcatcg tctggaccga cccacttctg gtgtgttgtt gatgggatta tccagcgagg
    23701 ccggacggct gctggcacaa cagtttgaac agcaccaaat ccagaaacgt taccatgcga
    23761 ttgtgcgcgg ctggttgatg gaagaagcgg tgctggatta tccactggtg gaagaactgg
    23821 acaaaatcgc tgataaattt gcccgcgaag ataaaggccc gcagccagca gtgacgcatt
    23881 atcgcggtct ggcgacagta gaaatgccgg tagcgaccgg acgttacccg accacgcgct
    23941 acggcctggt ggaactggag ccgaaaaccg gacgtaagca ccagctccgt cgccatcttg
    24001 cccatttgcg ccacccgatt attggcgaca gcaaacatgg tgatttacgc cagaatcgca
    24061 gcggtgctga acattttggc cttcagcggt taatgctgca tgccagtcag ttgtcattga
    24121 cgcatccttt tactggcgag ccgctgacta ttcacgcggg cctggacgac acctggatgc
    24181 aggcgttatc acaatttggc tggcggggtc tgcttcctga aaatgaaagg gttgagttca
    24241 gtgcgccatc gggtcaggat ggagagataa gttcctaatc cagggagaaa acggtatggc
    24301 ggaaattggt atttttgtcg gcactatgta cggaaattcg ctgttagtgg cggaagaagc
    24361 ggaagcaatt ctgaccgcgc agggccacaa agcaacggta tttgaagatc ctgaattaag
    24421 cgactggctg ccctatcagg ataagtatgt tctggtggtt acgtccacga ccgggcaggg
    24481 cgaccttcct gattgcattg tgccgctctt tcagggaatt aaagataccc tgggcttcca
    24541 gccgaatctg cgttatggcg tgattgcgct cggcgacagt agttatgtga atttctgcaa
    24601 tggcggcaaa cagttcgata ccttgttgca ggaacagagc gctcagcgag tgggagaaat
    24661 gctgttgatt gacgccagtg agaacccgga accggaaacg gaatcaaatc cgtgggtcga
    24721 acattggggc acgctgttgt cctgatacgt tcagcgagcg gtaaatgccg ttttagcagt
    24781 gctgaatcga atcttttcag gcaaatgcca gtaaaaactg cttcatagcg cggattttta
    24841 ctggcgtttg cctggagtca agcgatccat ttcatactct tctttatttc ttcgatttaa
    24901 cccttccttt cttgtttttg ttttcatttc cgtgaagtgg attccaccgt ccagggctaa
    24961 tgccaaaatc gggcctcatt gaacgcatta atgttgtgtt gttgcacggt gagccgctat
    25021 ggcgcgcttt ttatactgct attgccagat ataaacacgc gccgtattcg gcgaacgacc
    25081 tataaaaacg gcaaaaaaca ccctacgtca cctctgattt cctggcgatg tcgcagtcca
    25141 gagtgagcgt ggctaacgcg aattttcagg agtgcaacaa tgagttcttt aagtcaggct
    25201 gcgagcagtg tggaaaaacg cacgaatgct cgttactgga tagtggtgat gttgtttatc
    25261 gtcacatcct tcaactacgg cgaccgcgct acgctctcta tcgccggttc ggaaatggcc
    25321 aaagatatcg gccttgatcc tgtgggaatg ggctatgtgt tctctgcttt ctcatgggct
    25381 tatgttatcg ggcagatccc tggtggttgg ttgctggatc gttttggttc aaaacgcgtc
    25441 tacttctggt cgatctttat ctggtcgatg tttaccttgc tgcaaggctt cgtcgatatc
    25501 tttagtggat tcggcattat cgttgccctg tttacgctgc gcttcctggt cgggcttgct
    25561 gaagcgccct ctttccccgg caacagtcgc attgttgcgg cctggtttcc ggcgcaggaa
    25621 aggggaacgg cggtgtcgat ttttaactcc gctcaatact tcgcaacggt gatcttcgcg
    25681 ccgattatgg gctggctgac gcatgaagtg ggctggtcac acgtcttttt ctttatgggc
    25741 ggtctgggca ttgtcatcag ctttatctgg ttgaaagtca tccacgagcc aaatcaacat
    25801 ccgggtgtaa atcagaaaga gctggagtac atcgccgcgg gtggcgcgct gatcaatatg
    25861 gatcagcaaa acaccaaagt taaagtgccg ttcagcgtga agtgggggca gatcaaacag
    25921 ctgctcgggt cacggatgat gatcggcgtt tatatcggtc agtactgtat caacgccctg
    25981 acttacttct ttattacctg gttcccggtt tatctggtgc aggcgcgtgg gatgtcgatt
    26041 ctgaaagcgg gctttgtggc ttccgttccg gcggtttgcg gttttatcgg cggtgtgctg
    26101 ggtgggatta tttccgactg gctgatgcgc cgcacgggat cgctgaacat tgcgcgtaaa
    26161 acaccgatcg taatgggcat gttgctgtcg atggtgatgg tgttctgcaa ctacgtcaac
    26221 gttgagtgga tgatcatcgg ctttatggcg ctggccttct tcggtaaggg catcggggcg
    26281 ctgggttggg cagtaatggc agataccgcg ccaaaagaga tcagcggtct ttccggtggc
    26341 ctgttcaaca tgttcggtaa catttctggc atcgtcacgc caatcgcaat tggttatatc
    26401 gttggcacga ctggctcctt taatggggcg ctgatttatg ttggtgttca tgccttaatc
    26461 gcggtactga gctacctggt gttggtgggc gatatcaagc gtatcgagtt gaaacctgtt
    26521 gcggggcaat aagatggcga cacaatccag tcctgttatt actgatatga aagtcattcc
    26581 ggtggccggg catgacagca tgttgcttaa tattggtggt gcacataacg catatttcac
    26641 ccgcaatatt gtggtactca ccgataacgc cgggcatacc ggcattggtg aagcgccggg
    26701 cggagaggtg atttatcaga cactggtcga ggctattccg atggtgctgg gccaggaagt
    26761 tgcgcgcctg aataaagtgg ttcagcaggt gcataaaggt aatcaggcag ccgattttga
    26821 taccttcggc aaaggtgcct ggacttttga attgcgcgtt aatgccgtgg cggcgctgga
    26881 agccgccttg cttgacctgc taggtaaggc gctgaatgtt ccggtctgcg aactgttagg
    26941 gccaggcaag caacgtgatg ctattaccgt tctcggttat ctgttttata tcggtgatcg
    27001 gaccaaaacc gatcttcctt atctggaaaa tactccgggc aaccatgagt ggtatcaatt
    27061 gcggcatcag aaagcgatga acagcgaagc cgttgtgcgt ctggcggacg cctcacagga
    27121 tcgctatggc tttaaagatt tcaaacttaa gggcggagtg ttacctggcg agcaagaaat
    27181 cgacactgtt cgtgcattga agaaacgctt cccggatgcg cggattaccg ttgatcccaa
    27241 cggagcatgg ctgcttgatg aagctatttc tctgtgcaaa gggctgaatg atgttctcac
    27301 ctatgccgaa gatccgtgtg gcgcagaaca gggtttctcc ggacgtgaag tgatggcgga
    27361 atttcggcgg gcgaccggct tgcccgtcgc gactaatatg atcgccacca actggcgcga
    27421 aatgggccat gcggtgatgc tcaatgcggt agatattcca cttgccgacc cgcacttctg
    27481 gacgctttcc ggtgcagtcc gcgtagcgca gctttgcgac gactgggggc tgacctgggg
    27541 ctgccattcc aataaccact ttgatatctc tctggcgatg tttacccatg tgggcgcggc
    27601 ggcaccgggt aatcctaccg ctatcgatac ccactggatt tggcaggagg gcgattgtcg
    27661 cctgaccaaa aatccgctgg agattaaaaa tggaaaaatt gccgttcctg atgcgcccgg
    27721 tctgggcgtg gaactgaact gggagcaggt acaaaaggcg catgaggcct ataaacgtct
    27781 gcctggcggt gcgcgtaacg acgcgggccc gatgcagtac ctgatccccg gctggacctt
    27841 tgaccgtaaa cgtcccgttt tcggccgtca ttgattttga aaaaggacat aaagatgagt
    27901 tctcaatttt cgacgcctgt tgttactgaa atgcaggtca tcccggtggc gggccatgac
    27961 agtatgctga tgaatctgag tggtgcacac gcaccgttct tcacgcgtaa tattgtgatt
    28021 atcaaagata attctggtca cactggggta ggggaaattc ccggcggcga gaaaatccgt
    28081 aaaacgctgg aagatgcgat tccgctggtg gtgggtaaaa cgctgggtga atacaaaaac
    28141 gttctgacgc tggtgcgtaa tacatttgcc gatcgtgatg ccggcgggcg cggtttgcag
    28201 acatttgacc tgcggaccac tattcatgtg gttaccggga tagaagcggc aatgctggat
    28261 ctgctggggc agcatctggg ggttaacgtg gcatcgctgc tgggcgatgg tcaacagcgt
    28321 agcgaagtcg aaatgctcgg ttatctgttc ttcgtcggta atcgcaaagc cacaccgctg
    28381 ccgtatcaaa gccagccgga tgatcaatgt gactggtatc gcctgcgtca cgaagaagcg
    28441 atgacgccgg atgcggtggt gcgcctggcg gaagcggcat atgaaaaata tggcttcaac
    28501 gatttcaaac tgaagggcgg tgtactggcc ggggaagaag aagcagagtc tattgtggca
    28561 ctggcgcaac gcttcccaca ggcgcgtatt accctcgatc ctaacggtgc ctggtcgctg
    28621 aacgaagcga ttaaaattgg tacatacctg aaaggttcgc tggcttatgc agaagatccg
    28681 tgtggtgcgg agcaaggttt ctccgggcgt gaagtgatgg cagagttccg tcgcgcgact
    28741 ggcctgccga ccgcaaccaa tatgatcgcc accgactggc ggcagatggg ccatacgctc
    28801 tccctgcaat ccgttgatat cccgctggcg gatccgcatt tctggacgat gcaaggttcg
    28861 gtacgtgtgg cgcaaatgtg ccatgaattt ggcctgacct ggggttcaca ctctaacaac
    28921 cacttcgata tttctctggc gatgtttacc catgtagccg ctgctgcacc gggtaaaatt
    28981 actgctattg atacgcactg gatctggcag gaaggcaatc agcgtctgac caaagaaccg
    29041 tttgagatca aaggcgggct ggtacaggtg ccgcaaaaac caggattggg cgtagaaatc
    29101 gatatggatc aggtgatgaa agcccacgag ctgtatcaga aacatgggct tggtgcgcgt
    29161 gacgatgcga tgggaatgca gtacctgatt cctggctgga cgtttgataa caagcgcccg
    29221 tgcatggtgc gttaagactg aaaacaaaaa ccggctctgt caggagccgg tttgagactg
    29281 atgccaaacg caaaattgcc tgttgcgcta cgtatcaggc caacatactt tttaattcat
    29341 tgaatttaat tggttttgta ggcaggataa ggcgttaacg ccgcatccgg cataaacaca
    29401 ggcactttgt caccagtctg aaaccagctt cataaaaagc cgattgctac tcgacaagac
    29461 gtccgttacc cgagaatttt gctggcttcg cgcgctacat tatccatctc gtctaacagt
    29521 tccagcagct ccggctccaa atcttcttct ttagtaccgc tacgtagctg ttgttcgatc
    29581 agttggcaga gatttttcat acgcggcaca ccgctatagc cgcaactgcc atgcagttta
    29641 tgaatcaaat caaccaggcc ttccgggttt tctccaacca gctgttcctc aactttgttg
    29701 cgaacttcag gcaggaaatc gagtaacatt tgcagcatat cgcgcgctaa atcggttttt
    29761 cctgctgcct ggcgtagtgc cagttgccag tcgagggtcg cattcgggtt caccacaatt
    29821 tcgttgactt cgggcgtcac gacgcgagag gaaataccgc tgccaggctt atagcgcaac
    29881 aacaaattat gcaatcgctc ttcttcaatc ggtttcgcca gataatcgct catccctgcg
    29941 ccaagcagct tctctttttg cccggccatt gcatgcgccg ttaccgcgat aaccggcgtt
    30001 tgttgctgat gggggagctg gtggatgagt tcgcaggccc gaatgccgtc catgtccggc
    30061 atttgaatat ccattaagat caaatcgaac ggcatctgtt tcgcccgttc aaccgcctga
    30121 tgcccgctat cgcaaagttc cacatgttgc accatatctt ccagcaatgc gccgataagt
    30181 ttcaggttag cggggttgtc atcaaccgcc atgactgtca ttgccagctt actttcatcg
    30241 gttacaggca aaagcgtgtt ttgtttgtga tgacaaaatt ccgtcagggc aggtaacagg
    30301 cgcgtaggtg ttaatggttt cagcagacac gcgccgatac catcttgctt aagtttttca
    30361 gcattgactt gtgcatggca aggaagtgcc agcatcagaa aatcggtcat cgatactgct
    30421 ttcgctaatc gctcatgttg cattgttagc ggctcgcgga aggtcaccgc gatgcctaac
    30481 aacatcatgt cgtaatgcgc gggaggcagc gcggagaacg ttgggctata aaccacttcc
    30541 agcggcgttt cacttaaaat atccagcgtg cattgcgctg ctgcggagtt tggttcgaca
    30601 taggccaggc gcttaccggc gaggcactgg gtggatggcc cttcgataat aatgttcggg
    30661 ttcagatcga gattaatgtg gaaccagaaa gttgaaccac gattcggctg gctatggaac
    30721 gaaatatcac cgcccatttc attaaccagt ttttgtgtaa tcaccagccc cagcccggtg
    30781 ccaccatgac ggcgggaaat actggcatca gcctgtctga aggcctggaa taagcgcgat
    30841 tggtcgcgtt caggaatacc aatgccggta tcccgaatct gcacttcaat ttgcactttg
    30901 gtattactca gcgcacgttt ttctaccaga atatcaatgt tgccattctc ggtgaattta
    30961 attgcattcc ccaccaggtt agtgatgatt tgctgtaatc gtagtgggtc gccgataacg
    31021 ttatcaggca cgtcgctttt aatattgagc gtcagttcca gccctttatc gtgagaagaa
    31081 tgtgccagca gggtaacgac ttcatccagc gtgctgcgta gtgggaatgg aatactttcc
    31141 agaatcagct tacctgcttc cagtttcgag aagtcgagaa catcattaat aattgccagc
    31201 aaattatttg ccgaacgttc aatcgtattc aggtgatcgc gctgcgttgg tgttaattct
    31261 gttttcagcg tcaggcgggt aaagccaata acaccattca gtggtgtacg cagctcgtgt
    31321 gacatatttg ccagaaactc ggatttaata cgcgccgctt cctgggcgcg ctttttcgcc
    31381 agatccaact caacgttctg aatttccatc tgctccagcg tctcacgcag atcggacgtc
    31441 gcctggtcga tattgtgttg catctcttcg tgataagccg ccagcgacat tgccatcgag
    31501 ttgataccgt ttttcagcat atccagctcg ccgagcataa atccttccac ccggctgtcg
    31561 agttgcccgc gacggatgcg gtcgacggta ttcaccatgt tgcgaatcgg gccggtaaca
    31621 tcgcgcatta agcgccagcc aaaaataagc gcaataccga tacaaaacag catcatcacg
    31681 ctggaaataa agatctcttt atattgctgc aagcgaaccg atttaagatc cagctccagc
    31741 gcaatatatc ccagcatatt ttgactattt ttggcatcgc tactgggcga ttcgtcgggg
    31801 gagtaacttt cagaaataat cggcgtgcgg aggatcatta tatcgccgtc acgagtgaca
    31861 gtgagctggc gaggaaacgg cacgttgctg ccgagctgca ttgatgaggg atcaagatga
    31921 aaattggagg tgacaaagag tcggttattt tcatcataaa ccgaaatcgc gcgaacaata
    31981 tcagaatggc gacgatgcag tacgcttatt aactgaccga tagattcgcg attttgcaga
    32041 ctcatgccat attcagtaga aactgcaagc ggctcaataa tgctggcacc ggcatcttcc
    32101 agttgacgct gcaagtcgtt atagcgatgc acgacgaaaa agatactcag caataaacca
    32161 ataaggacgg tcggtgccag gatcagaatc atcatgcgtg cgcgcaggct gtagttggtc
    32221 atggagttcc gttatgggac aattaaggtc acactgttaa attgagaaag tctcgccaat
    32281 ggcgcaattc tactctgcaa aacgacgcac gacgacgcgt cagatcataa ccgtttcagt
    32341 caatgacctc gactcttttg gtcagggcgt ggcgcgacat aacggcaaaa cgttatttat
    32401 tcctggatta ttgccacagg aaaacgcgga agttactgtt actgaagata aaaaacagta
    32461 cgcccgcgct aaagtcgtac gccggttaag cgatagcccg gaacgcgaaa cgccacgctg
    32521 ccctcatttt ggcgtatgcg gtggctgtca gcaacaacac gccagcgagg atttacagca
    32581 gcgaagcaaa agtgcggcac tcgcccgatt aatgaaacac gaagtctctg aagtgatcgc
    32641 cgatgttccc tggggctatc gccgtcgcgc gcgtttaagt ttgaactact taccgaaaac
    32701 acagcaactt cagatggggt ttcgcaaagc gggctccagt gacattgtcg acgttaaaca
    32761 atgccccatt ttagtgcccc aacttgaagc attgctgccc aaagtcaggg catgcctggg
    32821 cagcttacaa gctatgcgcc atcttggtca tgttgaactg gtacaggcaa ccagcggcac
    32881 gctgatgatt ttgcgccata ccgcaccgct aagttcggta gatcgcgaaa aactggaacg
    32941 cttttcgcat tctgaaggcc tggatctgta tctcgccccc gatagtgaga tactcgaaac
    33001 cgtctctggt gagatgccct ggtatgactc aaacgggttg cgcttaactt ttagcccgcg
    33061 cgattttatt caggtcaatg cgggtgtgaa ccaaaaaatg gtagcgcgtg cgttggaatg
    33121 gctggatgta caacctgaag atcgcgtact ggatctgttc tgcggtatgg gcaactttac
    33181 actgccattg gcgacacaag ctgccagtgt ggtgggtgta gaaggcgttc cggcgctggt
    33241 ggaaaaaggc cagcagaatg cgcgtcttaa tggcttacac aatgtgacgt tttatcacga
    33301 aaatcttgaa gaagatgtca caaagcagcc gtgggcgaaa aacggcttcg ataaagtgtt
    33361 gctggacccg gcgcgagcag gtgccgcagg tgttatgcag caaattataa aactggaacc
    33421 tattcgtata gtttatgtat cctgtaatcc tgcaacgctg gctcgggata gcgaagcgtt
    33481 attaaaagca ggatatacca ttgcgcgact ggcgatgctg gatatgttcc cacacacggg
    33541 acatctggaa tcgatggtac ttttctcgcg cgttaaatag ttacgatttg ccgatttcgg
    33601 caggtctggt ccctaaagga gaggacgatg gttgcggtaa gaagtgcaca tatcaataag
    33661 gctggtgaat ttgatccgga aaaatggatc gcaagtctgg gtattaccag ccagaagtcg
    33721 tgtgagtgct tagccgaaac ctgggcgtat tgtctgcaac atacgcaggg gcatccggat
    33781 gccagtctgt tattgtggcg tggtgttgag atggtggaga tcctctcgac attaagtatg
    33841 gacattgaca cgctgcgggc ggcgctgctg ttccctctgg ctgatgccaa cgtagtcagc
    33901 gaagatgtgc tgcgtgagag cgtcggtaag tcggtcgtta accttattca cggcgtgcgt
    33961 gatatggcgg cgatccgcca gctgaaagcg acgcataccg attctgtttc ctccgaacag
    34021 gtcgataacg ttcgccggat gttattggcg atggtcgatg atttccgctg cgtggtcatc
    34081 aaactggcag agcgtattgc tcatctgcgc gaagtaaaag acgcgccgga agatgaacgc
    34141 gtactggcgg caaaagagtg caccaatatc tacgcgccgt tggcgaaccg tcttgggatt
    34201 gggcaactga aatgggagct ggaagattac tgcttccgtt atctccaccc gaccgaatac
    34261 aaacgcatcg caaaactgct gcatgaacgc cgtctcgacc gcgaacacta tatcgaagag
    34321 tttgtcggcc acctgcgcgc tgagatgaaa gctgaaggcg ttaaagctga agtgtatggt
    34381 cgtccgaaac acatctacag catctggcgc aaaatgcaga aaaagaacct cgccttcgat
    34441 gagctgtttg atgtgcgtgc ggtacgtatt gtcgccgagc gtttacagga ttgttatgcc
    34501 gcactgggga tagtgcacac tcactatcgc cacctgccgg atgagtttga cgattacgtc
    34561 gctaacccga aaccaaacgg ttatcagtct attcataccg tggttctggg accaggtggc
    34621 aaaaccgttg agatccaaat ccgcactaaa cagatgcatg aagatgcaga gttgggtgtt
    34681 gctgcgcact ggaaatataa agagggcgcg gcagctggcg gcgcacgttc gggacatgaa
    34741 gaccggattg cctggctgcg taaactgatt gcgtggcagg aagagatggc tgattccggc
    34801 gaaatgctcg acgaagtacg tagccaggtc tttgacgacc gggtgtacgt ctttacgccg
    34861 aaaggtgatg tcgttgattt gcctgcggga tcaacaccgc tggacttcgc gtaccacatc
    34921 cacagtgatg tcggacaccg ctgcatcggg gcaaaaattg gcgggcgcat tgtgccgttc
    34981 acctaccagc tgcagatggg cgaccagatt gaaattatca cccagaaaca gccgaacccc
    35041 agccgtgact ggttaaaccc aaatctcggt tacgtcacaa ccagtcgtgg gcgctcgaaa
    35101 attcacgcct ggttccgtaa acaggatcgt gacaaaaaca ttctggctgg acggcaaatc
    35161 cttgatgacg agctggaaca tctggggatc agcctgaaag aagcagaaaa acacctgctg
    35221 ccgcgttaca acttcaatga tgtcgacgag ttgctggcgg cgattggtgg cggggatatc
    35281 cgtctcaatc agatggtgaa cttcctgcaa tcgcaattta ataagccgag tgccgaagag
    35341 caggatgccg ccgcgctgaa acagcttcag caaaaaagct acacgccgca aaaccgcagt
    35401 aaagataacg gtcgtgtggt ggtagaaggt gtcggtaacc tgatgcacca catcgcgcgc
    35461 tgctgccagc cgattcctgg agatgagatt gtcggcttca ttacccaggg acgcggtatt
    35521 tcagtacacc gcgccgattg cgaacaactg gcggaactgc gctcccatgc gccagaacgc
    35581 attgttgacg cggtatgggg cgagagctac tccgccggat attcgctggt ggtccgcgtg
    35641 gtggctaatg atcgtagtgg gttgttacgt gatatcacga ccattctcgc caacgagaag
    35701 gtgaacgtac ttggcgttgc cagccgtagc gacaccaaac agcaactggc gaccatcgac
    35761 atgaccattg agatttacaa cctgcaagtg ctgggccgcg tgctgggtaa actcaaccag
    35821 gtgccggatg ttatcgacgc gcgtcggttg cacgggagtt aggccgaaac ttgttcgtat
    35881 ctacaatgta gatcgatata tactgtatct acatatgata gcggtttgag gaaagggtta
    35941 tgatccacag tagcgtaaag cgttggggaa attctcctgc agtgcggatc ccggctacgt
    36001 taatgcaggc gctcaatctg aatattgatg atgaagtgaa gattgacctg gtggatggaa
    36061 aattaattat tgagccagtg cgtaaagagc ccgtatttac gcttgctgaa ctggtcaacg
    36121 acatcacgcc ggaaaacctc cacgagaata tcgactgggg ggagccgaaa gataaggaag
    36181 tctggtaatg gtaagccgat acgtacccga tacgggcgat ctgatttggg ttgattttga
    36241 cccgacaaaa ggtagcgagc aagccggaca tcgtccggct gttgtcctga gtccgttcat
    36301 gtacaacaac aaaacaggta tgtgtctgtg tgttccttgt acaacgcaat caaaaggata
    36361 tccgttcgaa gttgttctat ccggtcagga acgtgatggc gtagcgttag ctgatcaggt
    36421 aaaaagtatc gcctggcggg caagaggagc aacgaagaaa ggaacagttg ccccagagga
    36481 attacaactc attaaagcca aaattaacgt gctgattggg taggtgttac taactcactt
    36541 ctattctggt cacaggtttc cacctgacag acccgcaatt ttcaggacaa ttcaatgaat
    36601 caaatcgacc gtttgctcac tattatgcag cgcctgcgcg atccggaaaa cggctgcccg
    36661 tgggataaag agcagacatt tgccaccatt gcgccttaca cccttgaaga aacctacgag
    36721 gtgctggacg ccatcgcccg tgaagatttt gacgatctgc gcggtgaact gggcgatctg
    36781 ctgttccagg tggtgtttta cgcacaaatg gctcaggaag aagggcgctt tgactttaat
    36841 gatatttgcg ctgctattag cgataaatta gagcgtcgcc atccgcatgt ttttgctgac
    36901 agttctgccg aaaacagtag tgaagtgctc gcccgttggg agcaaatcaa aaccgaagaa
    36961 cgcgcgcaga aagcacagca ttcggcgctg gatgatattc ctcgtagttt accggcttta
    37021 atgcgtgcgc aaaaaatcca gaaacgttgc gccaacgttg gcttcgactg gacgacgctt
    37081 ggtccggtag tcgataaagt ctacgaagag atcgacgagg tgatgtacga ggcgcagcag
    37141 gctgttgtcg accaggctaa actggaggag gaaatgggtg acctgctgtt tgccacggtc
    37201 aatctggctc gtcatttagg gacaaaagcg gaaatcgcat tgcaaaaagc gaacgaaaaa
    37261 ttcgagcgtc gttttcgcga agtggagcgt attgttgccg cgcgtggact ggaaatgaca
    37321 ggtgttgacc tcgaaacaat ggaagaagtc tggcaacagg taaaacggca ggaaattgat
    37381 ctctaaggga attacgcggt caagcgccat ttgtgtcatt ttttaaatga caagcgcttg
    37441 atttgcgtca aaaacattta ccccaaaggg gctattttct cactcctgat ttcaatagtg
    37501 cgctggcgaa gaggagggat aatgaaagtt tgtggcacag gtcatgttcg ggtatactgc
    37561 tttcccgtcc tggttattcc atcgtctttt caacctaact tctcaggttc agcatgacaa
    37621 cgaactatat ttttgtgacc ggcggggtcg tatcctctct gggtaaaggc attgccgcag
    37681 cctccctcgc agccattctt gaagcccgtg gcctcaatgt gaccatcatg aaactggatc
    37741 cgtacatcaa cgtcgatcca ggtactatga gcccaatcca acacggggaa gtgttcgtta
    37801 ctgaagacgg cgctgaaacc gacctggacc tggggcacta cgagcgtttc attcgtacca
    37861 aaatgagccg ccgcaacaac ttcaccacgg gtcgtatcta ctctgacgtt ctgcgtaaag
    37921 aacgccgtgg cgactacctc ggcgcaaccg tacaggttat tccgcacatc actaacgcta
    37981 tcaaagagcg cgtgctggaa ggcggcgaag gtcatgacgt ggtactggta gaaatcggcg
    38041 gtactgtagg tgatatcgaa tccctgccgt tccttgaagc gattcgccag atggctgttg
    38101 aaattggccg tgagcacacc ctgtttatgc acctgacgct ggtgccgtac atggcagcgt
    38161 ctggtgaagt caaaaccaaa ccgactcagc actctgtaaa agagctgctt tcaatcggta
    38221 tccagcctga tatcctgatt tgtcgttcag atcgcgccgt tccggcgaac gaacgtgcga
    38281 agattgcatt gttctgtaat gttccggaaa aagcggttat ttctctgaaa gacgtcgatt
    38341 ccatctataa aattccgggc ctgttgaaat ctcaggggct ggacgattat atttgtaaac
    38401 gattcagctt aaactgcccg gaagcgaatc tgtccgaatg ggaacaggtt atcttcgaag
    38461 aagcgaatcc ggtaagtgaa gtcaccattg gtatggtcgg caaatacatt gagctgccgg
    38521 atgcatacaa atccgtgatc gaagcactga aacacggtgg gctgaagaat cgtgtcagcg
    38581 tcaacatcaa actgatcgat tcacaagatg ttgaaacgcg cggcgttgaa atccttaaag
    38641 gtctggacgc aatcctcgta cctggcggtt tcggctatcg tggcgtagaa ggcatgatta
    38701 cgaccgcgcg ttttgcgcgt gagaacaata ttccttatct gggcatttgc ctgggtatgc
    38761 aggtggcgtt aattgattac gctcgccatg ttgccaacat ggagaacgcc aactctacgg
    38821 aatttgtgcc agactgtaag tacccggttg tggcgctgat taccgagtgg cgcgatgaaa
    38881 acggcaacgt tgaagttcgt agcgagaaga gcgatctcgg cggtaccatg cgtctcggcg
    38941 cacagcagtg ccagttggtt gacgatagcc tggttcgcca gctgtacaat gcgccgacaa
    39001 ttgttgagcg tcatcgtcac cgttacgaag tcaacaacat gctgttgaaa cagattgaag
    39061 atgcaggtct gcgcgttgcg ggccgttccg gggatgatca gttggtcgag atcatcgaag
    39121 ttccgaatca cccgtggttc gtggcttgcc agttccatcc ggagtttact tctactccac
    39181 gtgatggtca cccgctgttt gcaggctttg tgaaagccgc cagcgagttc cagaagcgtc
    39241 aggcgaagta agaaaaaagt tagaacggca acgcgtaccc tgggtacgcg ttgtttgtct
    39301 ggagtttcag tttaactagt gacttgagga aaacctaatg tccaaaatcg taaaaatcat
    39361 cggtcgtgaa atcatcgact cccgtggtaa cccgactgtt gaagccgaag tacatctgga
    39421 gggtggtttc gtcggtatgg cagctgctcc gtcaggtgct tctactggtt cccgtgaagc
    39481 tctggaactg cgcgatggcg acaaatcccg tttcctgggt aaaggcgtaa ccaaagctgt
    39541 tgctgcggta aacggcccga tcgctcaggc gctgattggc aaagatgcta aagatcaggc
    39601 tggcattgac aagatcatga tcgacctgga cggcaccgaa aacaaatcca aattcggcgc
    39661 gaacgcaatc ctggctgtat ctctggctaa cgccaaagct gctgcagctg ctaaaggtat
    39721 gccgctgtac gagcacatcg ctgaactgaa cggcactccg ggcaaatact ccatgccggt
    39781 tccgatgatg aacatcatca acggtggtga gcacgctgac aacaacgttg atatccagga
    39841 attcatgatt cagccggttg gcgcgaaaac tgtgaaagaa gccatccgca tgggttctga
    39901 agttttccat cacctggcaa aagttctgaa agcgaaaggt atgaacactg ctgttggtga
    39961 cgaaggtggc tatgcgccga acctgggttc caacgctgaa gctctggctg ttatcgctga
    40021 agctgttaaa gctgctggtt atgaactggg caaagacatc actctggcga tggactgcgc
    40081 agcttctgaa ttctacaaag acggtaaata cgttctggct ggcgaaggca acaaagcatt
    40141 cacctctgaa gaattcactc acttcctgga agaactgacc aaacagtacc cgatcgtttc
    40201 tatcgaagac ggtctggacg aatctgactg ggacggtttc gcataccaga ccaaagttct
    40261 gggcgacaaa atccagctgg ttggtgacga cctgttcgta accaacacca agatcctgaa
    40321 agaaggtatc gaaaaaggta tcgctaactc catcctgatc aaattcaacc agatcggttc
    40381 tctgaccgaa actctggctg caatcaagat ggcgaaagac gctggctaca ctgcagtaat
    40441 ctctcaccgt tctggtgaaa ctgaagatgc taccatcgct gacctggctg ttggtactgc
    40501 tgcaggccag atcaaaacgg gttctatgag ccgttctgac cgtgttgcta aatacaacca
    40561 gctgattcgt atcgaagaag ctctgggcga aaaagcaccg tacaacggtc gtaaagagat
    40621 caaaggccag gcataagtct gactttatct gatttaaaaa tgccagcctt cgggc
//
LOCUS       DAMZFT010000029        39138 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.029, whole genome shotgun sequence.
ACCESSION   DAMZFT010000029 DAMZFT010000000
VERSION     DAMZFT010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 39138)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39138)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39138
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.029"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            16..375
                     /gene="nrfF"
                     /locus_tag="PN977_001655"
     CDS             16..375
                     /gene="nrfF"
                     /locus_tag="PN977_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001440170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfF"
                     /protein_id="HDI6041697.1"
                     /translation="MLLFTCFARAQVVDTWQFANPQQQQQALNIASQLRCPQCQNQNL
                     LESNAPVAVSMRHQVYSMVEEGKSEVEIIGWMTERYGDFVRYNPPLTGQTLVLWALPV
                     VLLLLMALILWRVRAKR"
     gene            372..968
                     /gene="nrfG"
                     /locus_tag="PN977_001656"
     CDS             372..968
                     /gene="nrfG"
                     /locus_tag="PN977_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfG"
                     /protein_id="HDI6041698.1"
                     /translation="MKQPQIPVKMLTTLTILMVFLCIGSYLLSPKWQAVRAEYQRQRD
                     PLHQFASQQTPEAQLQALQDKIRANPQNSEQWALLGEYYLWQNDYSNSLLAYRQALQL
                     RGENAELYAALATVLYYQASQHMTAQTRAMIDKALALDSNEITALMLLASDAFMQANY
                     AQAIELWQKVMDLNSPRINRTQLVESINLAKLLQRRSD"
     gene            1310..2623
                     /gene="gltP"
                     /locus_tag="PN977_001657"
     CDS             1310..2623
                     /gene="gltP"
                     /locus_tag="PN977_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate:proton symporter GltP"
                     /protein_id="HDI6041699.1"
                     /translation="MKNIKFSLAWQILFAMVLGILLGSYLHYHSDSRDWLVVNLLSPA
                     GDIFIHLIKMIVVPIVISTLVVGIAGVGDAKQLGRIGAKTIIYFEVITTVAIILGITL
                     ANVFQPGAGVDMSQLATVDISKYQSTTEAVQSSSHGIMGTILSLVPTNIVASMAKGEM
                     LPIIFFSVLFGLGLSSLPATHREPLVTVFRSISETMFKVTHMVMRYAPVGVFALIAVT
                     VANFGFSSLWPLAKLVLLVHFAILFFALVVLGIVARLCGLSVWILIRILKDELILAYS
                     TASSESVLPRIIEKMEAYGAPASITSFVVPTGYSFNLDGSTLYQSIAAIFIAQLYGID
                     LSIWQEIILVLTLMVTSKGIAGVPGVSFVVLLATLGSVGIPLEGLAFIAGVDRILDMA
                     RTALNVVGNALAVLVIAKWEHKFDRKKALAYEREVLGKFDKTADQ"
     gene            complement(2815..3504)
                     /locus_tag="PN977_001658"
     CDS             complement(2815..3504)
                     /locus_tag="PN977_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HDI6041700.1"
                     /translation="MKKIIALMLFLTFFAHANDSEPGSQYLKAAEAGDRRAQYFLADS
                     WFSSGDLSKAEYWAQKAADSGDADACALLAQIKITNPVSLDYPQAKVLAEKAAQAGSK
                     EGEVTLAHILVNTQAGKPDYPKAISLLENASEDLENDSAVDAQMLLGLIYANGVGIKA
                     DDDKATWYFKRSSAISRTGYSEYWAGMMFLNGEEGFIEKNKQKALHWLNLSCMEGFDT
                     GCEEFEKLTNG"
     gene            complement(3598..5745)
                     /gene="fdhF"
                     /locus_tag="PN977_001659"
     CDS             complement(3598..5745)
                     /gene="fdhF"
                     /locus_tag="PN977_001659"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(5326..5328),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HDI6041701.1"
                     /translation="MKKVVTVCPYCASGCKINLVVDNGKIVRAEAAQGKTNQGTLCLK
                     GYYGWDFINDTQILTPRLKTPMIRRQRGGKLEPVSWDEALNYVAERLSAIKEKYGPDA
                     IQTTGSSRGTGNETNYVMQKFARAVIGTNNVDCCARVUHGPSVAGLHQSVGNGAMSNA
                     INEIDNTDLVFVFGYNPADSHPIVANHVINAKRNGAKIIVCDPRKIETARIADMHIAL
                     KNGSNIALLNAMGHVIIEENLYDKAFVASRTEGFEEYRKIVEGYTPESVEDITGVSAS
                     EIRQAARMYAQAKSAAILWGMGVTQFYQGVETVRSLTSLAMLTGNLGKPHAGVNPVRG
                     QNNVQGACDMGALPDTYPGYQYVKDPANREKFAKAWGVESLPAHTGYRISELPHRAAH
                     GEVRAAYIMGEDPLQTDAELSAVRKAFEDLELVIVQDIFMTKTASAADVILPSTSWGE
                     HEGVFTAADRGFQRFFKAVEPKWDLKTDWQIISEIATRMGYPMHYNNTQEIWDELRHL
                     CPDFYGATYEKMGELGFIQWPCRDTSDADQGTSYLFKEKFDTPNGLAQFFTCDWVAPI
                     DKLTDEYPMVLSTVREVGHYSCRSMTGNCAALAALADEPGYAQINTEDAKRLGIEDEA
                     LVWVHSRKGKIITRAQVSDRPNKGAIYMTYQWWIGACNELVTENLSPITKTPEYKYCA
                     VRVEPIADQRAAEQYVIDEYNKLKTRLREAALA"
     gene            complement(5943..7409)
                     /gene="mdtP"
                     /locus_tag="PN977_001660"
     CDS             complement(5943..7409)
                     /gene="mdtP"
                     /locus_tag="PN977_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter outer membrane
                     subunit MdtP"
                     /protein_id="HDI6041702.1"
                     /translation="MINRQLSRLLLCSILGSTTLISGCALVRKDSAPHQQLKPEQIKL
                     ADDIHLASSGWPQAQWWKQLNDPQLDALIQRTLSGSHTLAEAKLREEKAQSQADLLDA
                     GSQLQVAALGMLNRQRVSANGFLSPYAMDAPALGMDGPYYTEATVGLFAGLDLDLWGV
                     HRSAVAAAIGAHNAALAETAAVELSLTTGVAQLYYSMQASYQMLDLLEQTRDVIDYAV
                     KAHQSKVAHGLEAQVPFHGARAQILAVDKQIAAVKGQITETRESLRALIGAGASDMPE
                     IKPVALPRVQTGIPATLSYELLARRPDLQAMRWYVQASLDQVDSARALFYPSFDIKAF
                     FGLDSIHLDTLFKKTSRQFNFIPGLKLPLFDGGRLNANLEGTRAASNMMIERYNQSVL
                     NAVRDVAVNGTRLQTLNDEREMQAERVEATRFTQRAAEAAYQRGLTSRLQATEARLPV
                     LAEEMSLLMLDSRRVIQSIQLMKSLGGGYQAAPVVEKK"
     gene            complement(7406..9457)
                     /gene="mdtO"
                     /locus_tag="PN977_001661"
     CDS             complement(7406..9457)
                     /gene="mdtO"
                     /locus_tag="PN977_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313090.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter permease subunit
                     MdtO"
                     /protein_id="HDI6041703.1"
                     /translation="MSALNSLPLPVVRLLAFFHEELSERRPGRVPQTVQLWVGCLLVI
                     LISMTFEIPFVALSLAVLFYGIQSNAFYTKFVAILFVVATVLEIGSLFLIYKWSYGEP
                     LIRLIIAGPILMGCMFLMRTHRLGLVFFAVAIVAIYGQTFPAMLDYPEVVVRLTLWCI
                     VVGLYPTLLMTLIGVLWFPSRAITQMHQALNDRLDDAISHLTNSLTPLPETRIEREAL
                     ALQKLNVFCLADDADWRTQSAWWQSCVATVTYIYSTLNRYDPTSFADSQAIIEFRQKL
                     ASEINKLQHAIAEGQCWQSDWRITESQAMAARECNLENICQTLLQLGQMDPNTPPTPA
                     TKPPSMVADAFTNPDYMRYAVKTLLACLICYTFYSGVDWEGIHTCMLTCVIVANPNVG
                     SSYQKMVLRFGGAFCGAILALLFTLLVMPWLDNIVELLFVLAPIFLLGAWIATSSERS
                     SYIGTQMVVTFALATLENVFGPVYDLVEIRDRALGIIIGTVVSAVIYTFVWPESEART
                     LPQKLAGALGMLSKVMRIPRQQEVTALRTYLQIRIGLHAAFNACEEMCQRVALERQLD
                     SEERALLIERSQTVIRQGRDILHAWDATWNSAQALDNALQPDRAGQFADALEKYAAGL
                     ATALSRSPQITLEETPASQAILPTLLKQEQHVCQLFARLPDWTAPALTPATEQAQGAT
                     Q"
     gene            complement(9457..10488)
                     /gene="mdtN"
                     /locus_tag="PN977_001662"
     CDS             complement(9457..10488)
                     /gene="mdtN"
                     /locus_tag="PN977_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter periplasmic adaptor
                     subunit MdtN"
                     /protein_id="HDI6041704.1"
                     /translation="MESTPKKAPRSKFPALLVVALALVALVFVIWRVDSAPSTNDAYA
                     SADTIDVVPEVSGRIVELAVTDNQAVKQGDLLFRIDPRPYEANLAKAEASLAALDKQI
                     MLTQRSVDAQQFGADSVNATVEKARAAAKQATDTLRRTEPLLKEGFVSAEDVDRARTA
                     QRAAEADLNAVLLQAQSAASAVSGVDALVAQRAAVEADIALTKLHLEMATVRAPFDGR
                     VISLKTSVGQFASAMRPIFTLIDTRHWYVIANFRETDLKNIRSGTPATIRLMSDSGKT
                     FEGKVDSIGYGVLPDDGGLVLGGLPKVSRSINWVRVAQRFPVKIMVEKPDPEMFRIGA
                     SAVANLEPQ"
     gene            complement(10507..10782)
                     /locus_tag="PN977_001663"
     CDS             complement(10507..10782)
                     /locus_tag="PN977_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6041705.1"
                     /translation="MPTVLSRMAMQLKKTAWIIPVFMVSGCSLSPAIPVIGAYYPSWF
                     FCAIASLILTLITRRIIQRANINLAFVGIIYTALFALYAMLFWLAFF"
     gene            complement(10991..12976)
                     /locus_tag="PN977_001664"
     CDS             complement(10991..12976)
                     /locus_tag="PN977_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HDI6041706.1"
                     /translation="MNNSRLFRLSRIVIALTAASGMMVNTANATDEAKAATQYTQQVN
                     QNYAKSLPFSDRQDFDDAQRGFIAPLLDEGILRDANGKPYYRGEDYKFDINAPAPETV
                     NPSLWRQSQINGISGLFKVTDRMYQVRSQDISNITFIEGDTGIIVIDPLVTPNAAKAS
                     LDLYFKHRPQKPIVAVIYTHSHTDHYGGVKGIVSEADVKAGKVQIIAPAGFMDEAISE
                     NVLAGNIMSRRAFYSYGLLLPHNAQGDIGNGLGVTLTTGGPTIIAPTRSITKTGEKLN
                     IDGLDFEFLMAPGSEAPSEMHLYIPALKALCTAENSTHTLHNFYTLRGAKTRDTAKWT
                     DYLNETLDKWGSQAEVLFMPHTWPVWGNQHINDYIGKYRDTIKYIHDQTLHLANQGYT
                     MNEIGNMIHLPETLDKNWASRGYYGSVSHNARAVYNFYLGYYDGNPANLNPYGQVDMG
                     KRYVKALGGSAHAINLAREAYNQGDYRWASELLKQVIAANPGDQVAKNLQADTFEQLG
                     YQAESATWRGFYLTGAKELREGAKKIEHASTASPDTIKGMTVEMLLDYMAVRLNSEKA
                     AGKSISLNFNLSDNDNLNLSLNNSVLNYRKVLQPKVDASFYMSRSDLHDVLVGQAKMA
                     DLVKAKKAKIIGNGAKLEEIIACLDNFDLWVNIVTPN"
     gene            complement(13249..14178)
                     /gene="alsK"
                     /locus_tag="PN977_001665"
     CDS             complement(13249..14178)
                     /gene="alsK"
                     /locus_tag="PN977_001665"
                     /EC_number="2.7.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="allose kinase"
                     /protein_id="HDI6041707.1"
                     /translation="MQKQHNVVAGVDMGATHIRFCLRTAEGETLHCEKKRTAEVIAPD
                     LVSGIGEMIDEQLRRFNARCRGLVMGFPALVSKDKRTIISTPNLPLTAADLYDLADKL
                     ENTLNCPVEFSRDVNLQLSWDVVENSLTQQLVLAAYLGTGMGFAVWMNGAPWTGAHGV
                     AGELGHIPLGDMTQHCACGNPGCLETNCSGMALRRWYEQQPRNYPLSALFVHAENAPF
                     VQSLLENAARAIATSINLFDPDAVILGGGVMDMPTFPRETLIAMTQKYLRRPLPYQVV
                     RFIAASSSDFNGAQGAAILAHQRFLPQSCAKAP"
     gene            complement(14162..14857)
                     /gene="alsE"
                     /locus_tag="PN977_001666"
     CDS             complement(14162..14857)
                     /gene="alsE"
                     /locus_tag="PN977_001666"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-allulose-6-phosphate 3-epimerase"
                     /protein_id="HDI6041708.1"
                     /translation="MKISPSLMCMDLLKFKEQIEFIDSHADYFHIDIMDGHFVPNLTL
                     SPFFVSQVKKLASKPLDCHLMVTRPQDYIAQLARAGADFITLHPETINGQAFRLIDEI
                     RRHDMKVGLILNPETPVEAMKYYIHKADKITVMTVDPGFAGQPFIPEMLDKLAELKAW
                     REREGLEYEIEVDGSCNQATYEKLMAAGADVFIVGTSGLFNHAENIDEAWRIMTAQIL
                     AAKSEVQPHAKTA"
     gene            complement(14868..15848)
                     /gene="alsC"
                     /locus_tag="PN977_001667"
     CDS             complement(14868..15848)
                     /gene="alsC"
                     /locus_tag="PN977_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-allose ABC transporter permease"
                     /protein_id="HDI6041709.1"
                     /translation="MGFTTRVKSEASEKKPFNFALFWDKYGTFFILAIIVAIFGSLSP
                     EYFLTTNNITQIFVQSSVTVLIGMGEFFAILVAGIDLSVGAILALSGMVTAKLMLAGV
                     DPFLAALIGGVLVGGALGAINGCLVNWTGLHPFIITLGTNAIFRGITLVISDANSVYG
                     FSFDFVNFFAASVIGIPVPVIFSLIVALILWFLTTRMRLGRNIYALGGNKNSAFYSGI
                     DVKFHILVVFIISGVCAGLAGVVSTARLGAAEPLAGMGFETYAIASAIIGGTSFFGGK
                     GRIFSVVIGGLIIGTINNGLNILQVQTYYQLVVMGGLIIAAVALDRLISK"
     gene            complement(15827..17359)
                     /gene="alsA"
                     /locus_tag="PN977_001668"
     CDS             complement(15827..17359)
                     /gene="alsA"
                     /locus_tag="PN977_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-allose ABC transporter ATP-binding protein
                     AlsA"
                     /protein_id="HDI6041710.1"
                     /translation="MATPYISMAGIGKSFGPVHALKSVNLTVYPGEIHALLGENGAGK
                     STLMKVLSGIHEPTKGTITINNINYNKLDHKLAAQLGIGIIYQELSVIDELTVLENLY
                     IGRHLTKKICGVNIIDWREMRVRAAMMLLRVGLKVDLDEKVANLSISHKQMLEIAKTL
                     MLDAKVIIMDEPTSSLTNKEVDYLFLIMNQLRKEGTAIVYISHKLAEIRRICDRYTVM
                     KDGSSVCSGMVSDVSNDDIVRLMVGRELQNRFNAMKENVSNLAHETVFEVRNVTSRDR
                     KKVRDISFSVCRGEILGFAGLVGSGRTELMNCLFGVDKRAGGEIRLNDKDISPRSPLD
                     AVKKGMAYITESRRDNGFFPNFSIAQNMAISRSLKDGGYKGAMGLFHEVDEQRTAENQ
                     RELLALKCHSINQNITELSGGNQQKVLISKWLCCCPEVIIFDEPTRGIDVGAKAEIYK
                     VMRQLADDGKVILMVSSELPEIITVCDRIAVFCEGRLTQILTNRDDMSEEEIMAWALP
                     QE"
     gene            complement(17487..18422)
                     /gene="alsB"
                     /locus_tag="PN977_001669"
     CDS             complement(17487..18422)
                     /gene="alsB"
                     /locus_tag="PN977_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-allose transporter substrate-binding protein"
                     /protein_id="HDI6041711.1"
                     /translation="MNKYLKYFSGTLVGLMLSTSAFAAAEYAVVLKTLSNPFWVDMKK
                     GIEDEAKTLGVSVDIFASPSEGDFQSQLQLFEDLSNKNYKGIAFAPLSSVNLVMPVAR
                     AWKKGIYLVNLDEKIDMDNLKKAGGNVEGFVTTDNVAVGAKGASFIIDKLGAEGGEVA
                     IIEGKAGNASGEARRNGATEAFKKASQIKLVASQPADWDRIKALDVATNVLQRNPNIK
                     AIYCANDTMAMGVAQAVANAGKTGKVLVVGTDGIPEARKMVETGQMTATVAQNPADIG
                     ATGLKLMVDAEKSGKVIPLDKAPEFKLVDSILVTQ"
     gene            complement(18481..19371)
                     /gene="alsR"
                     /locus_tag="PN977_001670"
                     /gene_synonym="rpiR"
     CDS             complement(18481..19371)
                     /gene="alsR"
                     /locus_tag="PN977_001670"
                     /gene_synonym="rpiR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="als operon DNA-binding transcriptional repressor
                     AlsR"
                     /protein_id="HDI6041712.1"
                     /translation="MSQSEFDSALPNGIGLAPYLRMKQEGMTENESRIVEWLLKPGNL
                     SCAPAIKDVAEALAVSEAMIVKVSKLLGFSGFRNLRSALEDYFSQSEQVLPSELAFDE
                     APQDVVNKVFNITLRTIMEGQSIVNVDEIHRAARFFYQARQRDLYGAGGSNAICADVQ
                     HKFLRIGVRCQAYPDAHIMMMSASLLQEGDVVLVVTHSGRTSDVKAAVELAKKNGAKI
                     ICITHSYHSPIAKLADYIICSPAPETPLLGRNASARILQLTLLDAFFVSVAQLNIEQA
                     NINMQKTGAIVDFFSPGALK"
     gene            19730..20179
                     /gene="rpiB"
                     /locus_tag="PN977_001671"
     CDS             19730..20179
                     /gene="rpiB"
                     /locus_tag="PN977_001671"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional allose-6-phosphate
                     isomerase/ribose-5-phosphate isomerase RpiB"
                     /protein_id="HDI6041713.1"
                     /translation="MKKIAFGCDHVGFILKHEIVAHLVERGVEVIDKGTWSSERTDYP
                     HYASQVALVVAGGEADGGILICGTGVGISIAANKFAGIRAVVCSEPYSAQLSRQHNDT
                     NVLAFGSRVVGLELAKMIVDAWLGAQYEGGRHQQRVEAITAIEQRRN"
     gene            20248..20577
                     /gene="yjdP"
                     /locus_tag="PN977_001672"
     CDS             20248..20577
                     /gene="yjdP"
                     /locus_tag="PN977_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YjdP"
                     /protein_id="HDI6041714.1"
                     /translation="MKRFPLFLLFTLFTLSTVPAQADIIDDTIGNIQQAINAAYNPDR
                     GRDYEDSRDDGWQREVNDDRRRQYDDRRRQFEDRRRQLDDRQRQLDQERRQLEDEERR
                     MEDEYGR"
     gene            complement(20612..21370)
                     /gene="phnP"
                     /locus_tag="PN977_001673"
     CDS             complement(20612..21370)
                     /gene="phnP"
                     /locus_tag="PN977_001673"
                     /EC_number="3.1.4.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate metabolism protein PhnP"
                     /protein_id="HDI6041715.1"
                     /translation="MSLTLTLTGTGGAQGVPAWGCECAACARARRSPQYRRQPCSGVV
                     KFNDAITLIDAGLHDLADRWSPGSFQQFLLTHYHMDHVQGLFPLRWGVGDQIPVYGPP
                     DEQGCDDLFKHPGLLDFSHTMEPFVVFDLQGLQVTPLPLNHSKLTFGYLLETAHSRVA
                     WLSDTAGLPEKTLKFLRNNQPQVMVIDCSHPPREDAPRNHCDLNTVLALNQVIRSPRV
                     ILTHINHQFDAWLMENALPSGFEAGFDGMEIGVA"
     gene            complement(21372..21806)
                     /gene="phnO"
                     /locus_tag="PN977_001674"
     CDS             complement(21372..21806)
                     /gene="phnO"
                     /locus_tag="PN977_001674"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoalkylphosphonate N-acetyltransferase"
                     /protein_id="HDI6041716.1"
                     /translation="MPACELRPATQYDTDAVYALICELKQAEFDHHAFRVGFNANLRD
                     PNMRYHLALLDGEVVGMIGLHLQFHLHHVNWIGEIQELVVMPQARGLNVGSKLLAWAE
                     EEARQAGAEMTELSTNVKRHDAHRFYLREGYEQSHFRFTKAL"
     gene            complement(21793..22350)
                     /gene="phnN"
                     /locus_tag="PN977_001675"
     CDS             complement(21793..22350)
                     /gene="phnN"
                     /locus_tag="PN977_001675"
                     /EC_number="2.7.4.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 1,5-bisphosphokinase"
                     /protein_id="HDI6041717.1"
                     /translation="MTGKLIWLMGASGSGKDSLLTELRQREQTQLLVAHRYITRAASA
                     GSENHIALSEQEFFTRAGQNLLALSWHANGLYYGVGVEIDLWLHAGFDVVVNGSRAHL
                     PQARARYQSALLPVCLQVSPEILRQRLENRGRENASEINARLARAARYTPQDCLTLNN
                     DGSLRQSVDKLLTLIHQKEKHHACL"
     gene            complement(22350..23486)
                     /gene="phnM"
                     /locus_tag="PN977_001676"
     CDS             complement(22350..23486)
                     /gene="phnM"
                     /locus_tag="PN977_001676"
                     /EC_number="3.6.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate diphosphatase"
                     /protein_id="HDI6041718.1"
                     /translation="MIINNVKLVLENEVVHGSLEVQDGEIRAFAESQSRLPEAMDGEG
                     GWLLPGLIELHTDNLDKFFTPRPKVDWPAHSAMSSHDALMVASGITTVLDAVAIGDVR
                     DGGDRLENLEKMINAIEETQKRGVNRAEHRLHLRCELPHHTTLPLFEKLVQREPVTLV
                     SLMDHSPGQRQFANREKYREYYQGKYSLTDAQMQQYEEEQLALAARWSQPNRETIAAM
                     CRARHIALASHDDATHAHVAESHQLGSVIAEFPTTFEAAEASRKHGMNVLMGAPNIVR
                     GGSHSGNVAASELAQLGLLDILSSDYYPASLLDAAFRVADDESNRFTLPQAVKLVTKN
                     PAQALNLQDRGVIGEGKRADLVLAHRKGNHIHIDHVWRQGKRVF"
     gene            complement(23483..24163)
                     /gene="phnL"
                     /locus_tag="PN977_001677"
     CDS             complement(23483..24163)
                     /gene="phnL"
                     /locus_tag="PN977_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnL"
                     /protein_id="HDI6041719.1"
                     /translation="MINVQNVSKTFILHQQNGVRLPVLQRASLTVNAGECVVLHGHSG
                     SGKSTLLRSLYANYLPDEGQIQIKHGDEWVDLVTAPARKVVEIRKTTVGWVSQFLRVI
                     PRISALEVVMQPLLDTGVPREACAAKAARLLTRLNVPERLWHLAPSTFSGGEQQRVNI
                     ARGFIVDYPILLLDEPTASLDAKNSAAVVELIREAKTRGAAIVGIFHDEAVRNDVADR
                     LHPMGASS"
     gene            complement(24274..25032)
                     /gene="phnK"
                     /locus_tag="PN977_001678"
     CDS             complement(24274..25032)
                     /gene="phnK"
                     /locus_tag="PN977_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnK"
                     /protein_id="HDI6041720.1"
                     /translation="MNQPLLSVNNLTHLYAPGKGFSDVSFDLWPGEVLGIVGESGSGK
                     TTLLKSISARLTPQQGEIRYENRSLYGMSEADRRRLLRTEWGVVHQHPLDGLRRQVSA
                     GGNIGERLMATGARHYGDIRATAQKWLEEVEIPANRIDDLPTTFSGGMQQRLQIARNL
                     VTHPKLVFMDEPTGGLDVSVQARLLDLLRGLVVELNLAVVIVTHDLGVARLLADRLLV
                     MKQGQVVESGLTDRVLDDPHHPYTQLLVSSVLQN"
     gene            complement(25029..25874)
                     /gene="phnJ"
                     /locus_tag="PN977_001679"
     CDS             complement(25029..25874)
                     /gene="phnJ"
                     /locus_tag="PN977_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate 5-phosphate
                     C-P-lyase PhnJ"
                     /protein_id="HDI6041721.1"
                     /translation="MANLSGYNFAYLDEQTKRMIRRAILKAVAIPGYQVPFGGREMPM
                     PYGWGTGGIQLTASVIGESDVLKVIDQGADDTTNAVSIRNFFKRVTGVNTTERTDDAT
                     LIQTRHRIPETPLTEDQIIIFQVPIPEPLRFIEPRETETRTMHALEEYGVMQVKLYED
                     IARFGHIATTYAYPVKVNGRYVMDPSPIPKFDNPKMDMMPALQLFGAGREKRIYAVPP
                     FTRVESLDFDDHPFTVQQWNEPCAICGSTHSYLDEVVLDDAGNRMFVCSDTNYCRQQS
                     EAKNQ"
     gene            complement(25867..26931)
                     /gene="phnI"
                     /locus_tag="PN977_001680"
     CDS             complement(25867..26931)
                     /gene="phnI"
                     /locus_tag="PN977_001680"
                     /EC_number="2.7.8.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate synthase subunit PhnI"
                     /protein_id="HDI6041722.1"
                     /translation="MYVAVKGGEKAIDAAHALQESRRRGNTDLPELSVAQIEQQLNLA
                     VDRVMTEGGIADRELAALALKQASGDNVEAIFLLRAYRTTLAKLAVSEPLDTTEMRLE
                     RRISAVYKDIPGGQLLGPTYDYTHRLLDFTLLANGEAPTLTTADSEQQPSPHVFSLLA
                     RQGLAKFEEDSGAQPDDITRTPPVYPCSRSSRLQQLMRGDEGYLLALAYSTQRGYGRN
                     HPFAGEIRSGYIDVSIVPEELGFAVNVGELLMTECEMVNGFIDPPDEPPHFTRGYGLV
                     FGMSERKAMAMALVDRALQAPEYGEHATGPAQDEEFVLAHADNVEAAGFVSHLKLPHY
                     VDFQAELELLKRLQQERNHG"
     gene            complement(26931..27515)
                     /gene="phnH"
                     /locus_tag="PN977_001681"
     CDS             complement(26931..27515)
                     /gene="phnH"
                     /locus_tag="PN977_001681"
                     /EC_number="2.7.8.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnH"
                     /protein_id="HDI6041723.1"
                     /translation="MTLETAFMLPVQDAQHSFRRLLKAMSEPGVIVALHQLKRGWQPL
                     NIATTSVLLTLADNDTPVWLSAPLSNDIVSQSLRFHTNAPLVNQPELATFAVTDEAIS
                     SEQLNALSSGTAVAPEAGATLILQVVSLSGGRMLRLTGAGIAEERMIAPQLPECILHE
                     LTERPHPFPLGIDLILTCGERLLAIPRTTHVEVC"
     gene            complement(27512..27964)
                     /gene="phnG"
                     /locus_tag="PN977_001682"
     CDS             complement(27512..27964)
                     /gene="phnG"
                     /locus_tag="PN977_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnG"
                     /protein_id="HDI6041724.1"
                     /translation="MHADTATRQHWMSVLAHSQPTELAARLNALNITADYEVIRAAET
                     GLVQIQARMGGTGERFFAGDATLTRAAVRLTDGTLGYSWVLGRDKQHAERCALIDALM
                     QQTRHFQNLSETLIAPLNADRMARIAARQAEVNASRVDFFTMVRGDNA"
     gene            complement(27965..28690)
                     /gene="phnF"
                     /locus_tag="PN977_001683"
     CDS             complement(27965..28690)
                     /gene="phnF"
                     /locus_tag="PN977_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate metabolism transcriptional regulator
                     PhnF"
                     /protein_id="HDI6041725.1"
                     /translation="MHLSTHPTSYPTRYQEIAAKLEQELRQHYRCGDYLPAEQQLAAR
                     FEVNRHTLRRAIDQLVEKGWVQRRQGVGVLVLMRPFDYPLNAQARFSQNLLDQGSHPT
                     SEKLLSVLRPASGHVADALGITEGENVIHLRTLRRVNGVALCLIDHYFADLALWPTLQ
                     RFDSGSLHDFLREQTGIALRRSQTRISARRAQAKECQRLEIPNMSPLLCVRTLNHRDG
                     ESSPAEYSVSLTRADMIEFTMEH"
     gene            complement(28711..29430)
                     /gene="phnE"
                     /locus_tag="PN977_001684"
     CDS             complement(28711..29430)
                     /gene="phnE"
                     /locus_tag="PN977_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001395690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HDI6041726.1"
                     /translation="MVLAVLVVSWQGAEMAPLTLIKDGGNMATFAADFFPPDFSQWQD
                     YLTEMAVTLQIAVWGTALAVVLSIPFGLMSAENLVPWWVYQPVRRLMDACRAINEMVF
                     AMLFVVAVGLGPFAGVLALFIHTTGVLSKLLSEAVEAIEPGPVEGIRATGANKLEEIL
                     YGVLPQVMPLLISYSLYRFESNVRSATVVGMVGAGGIGVTLWEAIRGFQFQQTCALMV
                     LIIVTVSLLDFLSQRLRKHFI"
     gene            complement(29554..30570)
                     /gene="phnD"
                     /locus_tag="PN977_001685"
     CDS             complement(29554..30570)
                     /gene="phnD"
                     /locus_tag="PN977_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter substrate-binding
                     protein"
                     /protein_id="HDI6041727.1"
                     /translation="MNAKIIASLAFTSMFSLSTLLSPAHAEEQEKALNFGIISTESQQ
                     NLKPQWTPFLQDMEKKLGVKVNAFFAPDYAGIIQGMRFNKVDIAWYGNLSAMEAVDRA
                     NGQVFAQTVAADGSPGYWSVLIVNKDSPINNLNDLLAKRKDLTFGNGDPNSTSGFLVP
                     GYYVFAKNNISASDFKRTVNAGHETNALAVANKQVDVATNNTENLDKLKTSAPEKLKE
                     LKVIWKSPLIPGDPIVWRKNLSETTKDKIYDFFMNYGKTPEEKAVLERLGWAPFRASS
                     DLQLVPIRQLALFKEMQGVKSNKGLNEQDKLAKTTAIQAQLDDLDRLNNALSAMSSVS
                     KAVQ"
     gene            complement(30595..31383)
                     /gene="phnC"
                     /locus_tag="PN977_001686"
     CDS             complement(30595..31383)
                     /gene="phnC"
                     /locus_tag="PN977_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HDI6041728.1"
                     /translation="MQTIIRVEKLAKTFNQHQALHAVDLNIHHGEMVALLGPSGSGKS
                     TLLRHLSGLITGDKSAGSHIELLGRTVQREGRLARDIRKSRANTGYIFQQFNLVNRLS
                     VLENVLIGALGSTPFWRTCFSWFTREQKQRALQALTRVGMVHFAHQRVSTLSGGQQQR
                     VAIARALMQQAKVILADEPIASLDPESARIVMDTLRDINQNDGITVVVTLHQVDYALR
                     YCERIVALRQGHVFYDGSSQQFDNERFDHLYRSINRVEENAKAA"
     gene            complement(31516..31959)
                     /gene="yjdN"
                     /locus_tag="PN977_001687"
     CDS             complement(31516..31959)
                     /gene="yjdN"
                     /locus_tag="PN977_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001601143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family metalloprotein YjdN"
                     /protein_id="HDI6041729.1"
                     /translation="MPLSPYLSFAGNCADAIAYYQRTLGAELLYKISFGEMPKSAQDS
                     AENCPSGMQFPDTAIAHANVRIAGSDIMMSDAIPSGKASYSGFTLVLDSQQVEEGKRW
                     FDNLAANGKIEMAWQETFWAHGFGKVTDKFGVPWMINVVKQQPTH"
     gene            complement(32119..32454)
                     /locus_tag="PN977_001688"
     CDS             complement(32119..32454)
                     /locus_tag="PN977_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phnA family protein"
                     /protein_id="HDI6041730.1"
                     /translation="MSLPHCPKCNSEYTYEDNGMYICPECAYEWNDAEPAQESDELIV
                     KDANGNLLADGDSVTIIKDLKVKGSSSMLKIGTKVKNIRLVEGDHNIDCKIDGFGPMK
                     LKSEFVKKN"
     gene            32857..35085
                     /gene="crfC"
                     /locus_tag="PN977_001689"
     CDS             32857..35085
                     /gene="crfC"
                     /locus_tag="PN977_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="clamp-binding protein CrfC"
                     /protein_id="HDI6041731.1"
                     /translation="MYTQTLYELSQEAERLLQLSRQQLQLLEKMPLSVPGDDAPQLAL
                     PWSQPNIAERHAMLNNELRKISRLEMVLAIVGTMKAGKSTTINAIVGTEVLPNRNRPM
                     TALPTLIRHTPGQKEPVLHFSHVAPIDCLIQKLQQRLRDCDIKHLTDVLEIDKDMRAL
                     MQRIENGVAFEKYYLGAQPIFHCLKSLNDLVRLAKALDVDFPFSAYAAIEHIPVIEVE
                     FVHLAGLESYPGQLTLLDTPGPNEAGQPHLQKMLNQQLARASAVLAVLDYTQLKSISD
                     EEVREAILAVGQSVPLYVLVNKFDQQDRNSDDADQVRALISGTLMKGCITPQQIFPVS
                     SMWGYLANRARNELANSGKLPAPEQQRWVEDFAHAALGRRWRHADLANLEHIRHAADQ
                     LWEDSLFAKPIQALLHAAYANASLYALRSAAHKLLNYAQQAREYLDFRAHGLNVACEQ
                     LRQNIHQIEESLQLFQLNQAQVSGEIKHEIELALTSANLFLRQQQDAVNAQLAALFQD
                     DSGSLSEIRTCCETLLQTAQNTISRDFTLRFAELESTLCRVLTDVIRPIEQQVKMELS
                     ESGFRPGFHFPVFHSAVPHFNTRQLFSEVISRQDAMDEQSTRLGVVRETFSRWLNQPD
                     WGRGNEKSPTETVDYSVLQRALSAEVDLYCQQMAKVLAEQVDESVTAGMNTFFAEFAS
                     CLTELQTRLRESLALRQQNESVVRLMQQQLQQAVMTHSWIYTDAQLLRDDIQTLFTAE
                     RY"
     gene            35082..35960
                     /locus_tag="PN977_001690"
     CDS             35082..35960
                     /locus_tag="PN977_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjcZ-like family protein"
                     /protein_id="HDI6041732.1"
                     /translation="MTKTLLDGPGRVLESVHPRFLVDLAQGDDARHPQAHQQQFRERL
                     MQELLARVQLQAWTNSGMLNAPLSLRLTLVEKLASMLDPGHLALTQIAQHLALLQKMD
                     HRQHSAFPELPQQIAALYEWFSARCRWKEKALTQRGLLVQAGEQSEQIFTRWRAGAYN
                     AWSLPGRCFIVLEELRWGAFGDACRLGSPQAVALLLGDLRVKATQHLAESINAAPTTR
                     HYYHQWFASSTVSTGGDHADFLSWLGKWTTADKQPVCWSVTQRWQTVALGMPRLCSAQ
                     RLAGAMVEEIFSVNLV"
     gene            36224..37726
                     /gene="proP"
                     /locus_tag="PN977_001691"
     CDS             36224..37726
                     /gene="proP"
                     /locus_tag="PN977_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline transporter ProP"
                     /protein_id="HDI6041733.1"
                     /translation="MLKRKKVKPITLRDVTIIDDGKLRKAITAASLGNAMEWFDFGVY
                     GFVAYALGKVFFPGADPSVQMVAALATFSVPFLIRPLGGLFFGMLVDKYGRQKILAIT
                     IVIMSISTFCIGLIPSYDTIGIWAPILLLICKMAQGFSVGGEYTGASIFVAEYSPDRK
                     RGFMGSWLDFGSIAGFVLGAGVVVLISTIVGEENFLDWGWRIPFFIALPLGIIGLYLR
                     HALEETPAFQQHVDKLEQGDREGLQDGPKVSFKEIATKYWRSLLTCIGLVIATNVTYY
                     MLLTYMPSYLSHNLHYSEDHGVLIIIAIMIGMLFVQPVMGLLSDRFGRRPFVLLGSVA
                     LFVLAIPAFILINSNVIGLIFAGLLMLAVILNCFTGVMASTLPAMFPTHIRYSALAAA
                     FNISVLVAGLTPTLAAWLVESSQNLMMPAYYLMVVAVVGLITGVTMKETANRPLKGAT
                     PAASDIQEAKEILVEHYDNIEQKIDDIDHEIADLQAKRTRLVQQHPRIDE"
     gene            37838..37927
                     /gene="pmrR"
                     /locus_tag="PN977_001692"
     CDS             37838..37927
                     /gene="pmrR"
                     /locus_tag="PN977_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000797656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LpxT activity modulator PmrR"
                     /protein_id="HDI6041734.1"
                     /translation="MKNRVYESLTTVFSVLVVSSFLYIWFTTY"
     gene            complement(37903..39003)
                     /gene="pmrB"
                     /locus_tag="PN977_001693"
     CDS             complement(37903..39003)
                     /gene="pmrB"
                     /locus_tag="PN977_001693"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PmrB"
                     /protein_id="HDI6041735.1"
                     /translation="MNLMRFLRRPISLRQRLILTIGAILLVFELISVFWLWHESTEQI
                     QLFEQALRDNRNNDRHIMREIREAVASLIVPGVFMVSLTLFICYQAVRRITRPLAELQ
                     KELEARTADNLTPIAIHSATLEIEAVVSALNDLVSRLTSTLDNERLFTADVAHELRTP
                     LAGVRLHLELLAKTHHIDVAPLVARLDQMMESVSQLLQLARAGQSFSSGNYQHVKLLE
                     DVILPSYDELSTMLDQRQQTLLLPESAADITVQGDATLLRMLLRNLVENAHRYSPQGS
                     NIMIKLQEDGGAVMAVEDEGPGIDESKCGELSKAFVRMDSRYGGIGLGLSIVSRITQL
                     HHGQFFLQNRQETSGTRAWVRLKKDQYVVNQI"
     gene            complement(39004..>39138)
                     /locus_tag="PN977_001694"
     CDS             complement(39004..>39138)
                     /locus_tag="PN977_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HDI6041736.1"
                     /translation="YNWDNEPSTNTLEVHIHNLRDKVGKARIRTVRGFGYMLVANEEN
                     "
BASE COUNT         8708 a        10257 c        10644 g         9529 t
ORIGIN      
        1 ggacttctca cgctactgct gttatttacc tgttttgcgc gtgctcaggt cgtagacacc
       61 tggcaattcg ccaatccgca acaacagcaa caggcgttaa atattgccag ccagttacgt
      121 tgtccgcagt gtcagaatca gaacttactg gaatccaatg cgccggtggc ggtcagtatg
      181 cgccatcagg tttacagcat ggtggaagag gggaaaagtg aagtcgaaat cattggctgg
      241 atgaccgaac gctacggaga ttttgttcgc tataacccac cgttaacggg tcagacgctg
      301 gtattgtggg ctctgccagt ggtgttgtta ctgctgatgg cactgatcct ctggcgagtg
      361 agggcgaagc gatgaaacag cctcaaatac cggtgaaaat gctgacaaca ctcacgattt
      421 tgatggtatt tctctgtatc ggcagttatc tgttaagccc aaaatggcag gcggtacgtg
      481 cggagtatca acgtcagcgc gatccgctac atcagtttgc cagccagcaa accccggaag
      541 cgcagcttca ggcattgcag gataaaatcc gcgctaatcc acaaaacagc gaacaatggg
      601 cgttactggg cgagtattat ctgtggcaaa acgattacag caattcgctg ctggcgtacc
      661 gtcaggcgtt gcaactacgt ggtgagaacg ctgaactgta tgcggcgctg gcgacggtgc
      721 tttattacca ggccagccag catatgaccg cccagactcg cgcaatgatc gacaaagccc
      781 tcgcgctgga ctctaacgaa atcaccgctc tgatgctgct ggcttccgat gcgtttatgc
      841 aggcgaatta cgcgcaagcc atcgaactat ggcaaaaagt gatggatctc aactcacccc
      901 ggatcaaccg gacacagctg gtcgagtcga ttaatttggc gaaactgttg cagcggagat
      961 cagattaatc atctggtgat gaagattatg ttattgagct gcataaaaaa taatcgaatg
     1021 aacatatgcc caaaataatc actaatcagt attattgcaa attaaacaaa taaaaatctt
     1081 tctataacaa atggttattc attaatcctg ccataaactc gcgttattat gcattaatgc
     1141 agcgaaaagc tctgttgtta aagggttgcg caacataccg cgcaaatgat actgatcata
     1201 agcgttaaaa aaatctacaa accaacgcaa cacaattcat gccctggcag tatgtcacgt
     1261 tctcgcgttt ctgaacgggg aacggcgctc cattgaggaa gttattctga tgaaaaatat
     1321 aaaattcagc ctggcctggc agattctgtt tgctatggtg ctgggcattc tcctgggaag
     1381 ctacctgcac taccatagcg acagtcgcga ctggctggtc gtcaatttgc tctctccggc
     1441 gggtgatatc ttcatccatc tgatcaaaat gattgttgtg ccgattgtga tctccacgct
     1501 ggtggtgggt atcgcgggtg ttggtgatgc caaacagctc gggcgtattg gcgcgaaaac
     1561 cattatttac ttcgaggtga tcaccaccgt cgccatcatt ttggggatta ctctggcgaa
     1621 cgtcttccag cccggtgccg gggtggatat gtcgcagctg gcgaccgtcg atatctcgaa
     1681 atatcagagc actacggaag cggtacaaag cagttcccac ggcattatgg gcacgatttt
     1741 gtcgctggtg ccgacgaaca ttgtggcgtc gatggcgaaa ggcgaaatgc tgccgatcat
     1801 cttcttctcg gtgctgtttg gtctggggct ttcatccctg cccgcgacgc atcgtgaacc
     1861 gctggtgacc gtgttccgct ccatctctga aaccatgttt aaagtgactc acatggtgat
     1921 gcgttatgca ccggtgggtg tgtttgcgct gattgcggtg acggtggcta actttggttt
     1981 ctcttctctg tggccactgg cgaaactggt gctgctggtg catttcgcca ttctgttctt
     2041 cgcgctggta gtgctgggaa ttgtggcgcg cctgtgcggg ttaagcgtct ggatcctgat
     2101 tcgtattctg aaagatgagc tgattctggc gtactccact gccagttctg aaagcgtgct
     2161 gccgcgaatt attgagaaga tggaagccta cggtgcaccg gcgtcgatca ccagtttcgt
     2221 ggtgccgacc ggttactctt ttaaccttga tggttcgacg ctgtatcaaa gtattgccgc
     2281 tattttcatc gcgcagttgt atggcattga cctgtccatc tggcaggaaa tcattctggt
     2341 gctgacgctg atggtgacct cgaaagggat tgctggcgtg ccgggcgtgt cgtttgtggt
     2401 gctgctggca acgctgggta gcgtaggtat tccgctggaa ggtctggcat ttattgccgg
     2461 tgttgaccgt atcctcgata tggcgcgtac tgcgctgaac gtggtgggta atgcgctggc
     2521 ggtgctggtg attgccaagt gggaacacaa atttgaccgt aagaaagcgc tggcttatga
     2581 gcgcgaagtg ctgggcaaat ttgataaaac tgcggatcaa taattgaaga ttgccgggga
     2641 tatccacccg gcaatgtgtg gatgcctgat gcgacgcttg ccgcgtctta tcaggcctac
     2701 gtcagacagc gcaatagcct gatttagcgt gattttgtag gtcggataag gcgtttacgc
     2761 cgcatccgac atcaacgcct gatgcgacga ttaacgcgtc ttatcaggcc tacattaacc
     2821 gttagttaat ttttcaaact cttcacaccc ggtatcaaac ccttccatac agctcagatt
     2881 caaccagtgc agcgccttct gtttgttctt ctcgataaaa ccttcttcac cgtttaagaa
     2941 catcattccc gcccagtact cggaataacc ggttcgggaa atcgcagagc tgcgtttgaa
     3001 ataccaggtt gccttgtcat cgtcggcctt aatgcccacg ccgttggcgt aaatcaaacc
     3061 aagcagcatt tgggcatcga cggcagagtc gttctccaga tcttccgagg cgttttctaa
     3121 cagcgaaatc gcctttggat aatccggttt acccgcttgc gtatttacca gaatatgcgc
     3181 cagcgttact tcaccttctt tactgcccgc ttgcgccgct ttctctgcaa gaacttttgc
     3241 ttgtggatag tccagactga ccggattggt gattttgatc tgcgctaaca gcgcgcaggc
     3301 atcagcatca ccgctgtcgg cggctttctg tgcccaatat tcggctttgc tcaaatcgcc
     3361 ggagctaaac cagctgtcgg caagaaaata ttgtgcgcgt cggtccccag cctctgctgc
     3421 ctttaaatac tggctgccag gctcggagtc gttcgcgtgg gcaaagaatg tcaaaaacaa
     3481 catcaatgcg ataatttttt tcattttgat aatacgattg ggtagggagt aaccagtata
     3541 aagagttcga atggataaaa aaacagcctc cgaaaggagg ctgtagaaag gactgtatta
     3601 cgccagtgcc gcttcgcgca ggcgagtttt caacttgttg tactcgtcaa tcacatactg
     3661 ctcggcggcg cgctgatcgg cgatcggctc gacgcgcacg gcgcagtatt tgtattccgg
     3721 cgttttcgta atcgggctta agttttcggt aaccagctcg ttacaggcac caatccacca
     3781 ctggtaggtc atgtaaatcg cccctttgtt cggacgatcg ctgacctgcg cacgggtgat
     3841 aattttgcct ttacgcgagt gcacccaaac caatgcctca tcttcaatcc ccagacgttt
     3901 ggcgtcttcg gtattgattt gtgcgtagcc aggttcatca gccagcgccg ccagtgccgc
     3961 acagttaccg gtcatcgaac ggcaagagta gtgaccgact tcacgcaccg ttgacagtac
     4021 catcggatac tcgtcggtga gtttgtcgat tggcgctacc cagtcgcagg tgaagaactg
     4081 cgccagaccg ttcggagtat caaacttctc tttaaacaga taagaagtcc cctgatcggc
     4141 atctgaagta tcgcgacaag gccactgaat gaagcccagt tcgcccattt tctcgtaagt
     4201 cgcaccgtag aaatccgggc acagatgacg caactcatcc cagatctcct gggtgttgtt
     4261 gtagtgcatc ggataaccca tacgggtggc gatttcactg atgatttgcc agtccgtttt
     4321 caaatcccat ttcggttcaa ctgctttaaa gaaacgctgg aagccacggt cagccgcagt
     4381 aaacacgcct tcatgctcgc cccatgacgt tgacggtaaa atgacatccg ccgccgacgc
     4441 ggttttggtc ataaagatgt cctgaacgat gaccagttcc agatcttcaa aggctttacg
     4501 tactgccgac agctccgcgt cagtttgtag cggatcttcg cccataatgt acgcggcacg
     4561 cacttcacca tgcgctgcgc ggtgcggcag ctcgctgatg cgataaccgg tatgagcagg
     4621 caggctttcc acgccccagg ctttggcgaa tttctcgcgg ttagccggat ctttcacgta
     4681 ctggtatccc ggatacgtat ccggcagcgc gcccatatcg caggcaccct gtacgttgtt
     4741 ctgaccacga accgggttta cacccgcatg tggcttaccg aggttaccgg tcagcatcgc
     4801 gaggctggtc agagaacgca cggtttccac gccctggtag aactgggtta cacccatgcc
     4861 ccacaggatg gcggcgcttt tcgcctgggc atacatccgt gccgcctgac gaatctcact
     4921 ggcgctgacg ccggtgatat cttcaaccga ctccggcgtg tagccttcaa cgattttacg
     4981 atactcttca aagccttctg tacgggaagc gacgaacgct ttgtcgtaca gattttcttc
     5041 aataatgaca tggcccatcg cattcaacag cgcgatgttc gagccgtttt tcaatgcaat
     5101 gtgcatgtca gcaatgcgcg cagtttcaat tttgcgcgga tcgcagacga taattttcgc
     5161 cccgttacgt ttagcgttaa ttacgtggtt cgccacgatt gggtgggaat ccgccgggtt
     5221 atacccgaaa acgaacacta aatcggtatt atcaatttcg ttaatagcat tgctcattgc
     5281 gccattaccg accgattggt gcagacctgc aaccgatggg ccgtgtcaga cacgagcgca
     5341 gcagtcaacg ttattggtac caataacggc gcgcgcaaat ttttgcatta catagttggt
     5401 ttcgttaccc gtaccacgcg aggagccggt cgtctggatg gcatccggac cgtacttctc
     5461 tttgatggcg ctcaggcgct cggcaacgta attcagtgcc tcatcccagg aaacaggttc
     5521 gagtttgccg ccacgctggc gacggatcat gggggttttc aggcgcgggg tcaggatctg
     5581 ggtatcgtta atgaagtccc agccataata acccttcaga cacagggtac cctggttggt
     5641 tttcccctgc gctgcctccg cccggacgat tttgccgtta tcgacgacca ggttgatttt
     5701 gcaacctgat gcgcaatagg ggcaaaccgt gacgactttt ttcatcggtc tcgctccagt
     5761 taatcaaatc acgcatacgc gctctcgact acagtatgca tcttttatgc cacattttat
     5821 ttggggtcat tccctgatat tacgggcact atttattcaa aactctgacg aaaaacaggc
     5881 tgtcgtcagt tttgacgtga cgaaacgaaa taccgcgtga cagccatcac gcggtagaca
     5941 ttttattttt tctcgacgac gggagccgcc tgatacccgc cgcccagcga tttcatcaac
     6001 tgaatgcttt ggatcacccg gcggctgtcc agcatcagta atgacatctc ctcggcaagc
     6061 actggcaacc gggcttcggt ggcctgtaag cggctggtta agccgcgttg ataggcggcc
     6121 tcggcagcgc gctgggtgaa gcgcgttgct tccacgcgtt cagcctgcat ctctcgctcg
     6181 tcgttaagcg tttgcagacg cgtgccgttg acggcaacgt cgcgcaccgc gttcagtact
     6241 gactggttgt aacgttcaat catcatgttg ctggcggcgc gcgtgccttc gagattggca
     6301 ttcaaccgtc caccgtcaaa cagcggcaat ttcagacctg ggataaagtt gaactggcga
     6361 ctggtttttt tgaataaggt atccaggtgg atagaatcca ggccgaaaaa cgctttgata
     6421 tcaaagctcg gatagaacag cgcccgcgcg gaatccacct gatctaatga cgcctgaaca
     6481 taccagcgca tggcttgcag atccgggcgt ctggcgagca actcataaga gagtgttgcc
     6541 ggaatgccgg tctggactcg cggtaatgcc accggtttga tctccggcat atcgctggcg
     6601 cccgcgccaa tcaatgcgcg cagagattcc cgcgtttcag tgatttgccc tttgacggcg
     6661 gcaatttgtt tatcgacggc cagaatttgc gcccgcgcgc cgtggaaagg cacttgcgct
     6721 tccagaccgt gcgccacttt actttggtgc gctttcaccg cgtaatcaat cacatcgcgt
     6781 gtttgttcta acagatcgag catctgatag ctggcctgca tactgtaata aagctgcgct
     6841 acgcccgtgg tcagcgatag ctctactgct gcggtttctg ccagcgctgc attatgcgcg
     6901 ccaatggcgg cggcaaccgc tgagcgatgc acaccccaca aatcgagatc cagtccggca
     6961 aacaaaccta ctgtggcttc cgtatagtac ggcccgtcca tccccagtgc gggcgcatcc
     7021 atcgcataag ggcttaaaaa gccgttcgcc gagacgcgtt ggcggttaag catccctaac
     7081 gctgccacct gcaactgtga accggcatct aacaaatcgg cctgcgattg cgctttttct
     7141 tcccgcagtt tcgcttcggc gagggtgtgt gaaccactta gcgtccgttg gatcaaagca
     7201 tccaactgcg ggtcattgag ttgtttccac cactgcgcct gcggccagcc ggagctggca
     7261 agatgaatat catcggccag tttgatttgt tccggtttga gctgttgatg aggcgcagaa
     7321 tccttacgta ccagggcaca gccggaaatc agcgtcgtgc tgccgagaat gctgcacaac
     7381 agcaggcgtg aaagttgacg attgatcatt gcgtggctcc ttgtgcctgt tccgtggcgg
     7441 gcgttaatgc cggggctgtc cagtctggca agcgggcgaa aagctggcag acgtgttgct
     7501 cctgttttaa taaggtcggc aggatggcct gcgaggcggg tgtttcttca agcgttattt
     7561 gaggagaacg actgagtgcg gttgccagac cggcagcgta tttctccagg gcgtcggcaa
     7621 actgaccagc tctgtccggc tgtagtgcgt tatccagcgc ctgcgccgag ttccaggttg
     7681 catcccaggc gtgaagaata tcgcgaccct gacgaataac cgtttgcgaa cgttcaatca
     7741 gtaaggcgcg ttcttcgctg tccagttgac gctccagcgc cacgcgttgg cacatctctt
     7801 cacaggcatt aaacgccgca tgcagaccta tgcgaatttg cagataagtg cgcagagccg
     7861 tgacttcctg ctggcgtggg atccgcatta ctttacttaa catacccagc gcgccagcca
     7921 gtttttgcgg cagggtgcgc gcttcacttt caggccagac aaaggtgtaa atcaccgcgg
     7981 acaccacggt accaatgatg atacccaggg cgcgatcgcg aatttccacc aggtcgtaca
     8041 ccgggccaaa aacattttcg agcgtggcga gcgcgaaggt gaccaccatc tgtgtgccga
     8101 tataagaaga gcgttcagag ctggtggcaa tccatgcgcc caacaggaaa atcggtgcca
     8161 gcacaaacag caattcgaca atattgtcca gccagggcat gaccagtagc gtgaataaca
     8221 gcgccagaat cgcaccgcaa aaggccccgc caaaacgcag caccatcttc tggtacgacg
     8281 aaccgacatt tggattagcg acgatcacgc aggtcagcat acaggtgtga atgccttccc
     8341 agtccacgcc gctgtagaag gtgtaacaga tcaaacaggc gagcagcgtt tttaccgcgt
     8401 agcgcatata gtctggattg gtaaaagcgt cggcgaccat tgatggcggt ttggtggcgg
     8461 gcgttggcgg cgtattcggg tccatctgac ccagttgcaa caatgtctgg cagatattct
     8521 ccaggttaca ttcccgtgcc gccatcgctt gactttcagt gatccgccag tcgctttgcc
     8581 agcactgacc ttcggcaatg gcatgctgca gcttgttgat ttctgaggct aatttttgcc
     8641 ggaattcaat aattgcctga gaatcagcaa aagaggtggg atcgtagcga ttcagcgtcg
     8701 agtaaatgta ggttaccgtt gccacacagc tttgccacca tgcgctctga gtacgccagt
     8761 cggcatcgtc cgcgaggcaa aagacattga gtttttgcag cgccagcgcc tctctttcaa
     8821 tccgcgtttc gggtagcggt gtgaggctgt tcgtcaggtg gctaatggca tcatcaagcc
     8881 gatcattaag cgcctgatgc atctgcgtga tagcgcggct gggaaaccac agcacgccga
     8941 ttaacgtcat cagcaaggtt ggatagaggc caacaacgat acaccacagc gttaagcgca
     9001 cgaccacttc cggatagtcg agcatggcgg ggaaggtttg cccgtaaatg gcgacaatgg
     9061 cgacagcgaa aaagaccagt cccagccgat gggtgcgcat caaaaacata catcccatca
     9121 ggatcggccc tgcgatgatc aatcggatca acggttcgcc gtatgaccat ttgtagatca
     9181 aaaacaggct gccgatctcc agcaccgtgg caaccacaaa caagatcgcg acaaatttgg
     9241 tgtaaaacgc gttggactga ataccgtaaa acagcaccgc cagcgataac gccacaaagg
     9301 ggatctcgaa ggtcatcgag atcagaatca ccagcaggca gcctacccag agttgcacgg
     9361 tctgcggcac acgacctggc cgccgctcgc ttaactcttc atgaaagaac gccagcagcc
     9421 tgaccaccgg taatggcagg gagttgagcg cgctcattat tgtggctcaa gattagcgac
     9481 tgccgaagcg ccgatgcgga acatttccgg gtcaggtttt tcaaccatga ttttgaccgg
     9541 aaaacgctgg gcaacgcgga cccagttaat agaacgagac actttcggca ggccgcccag
     9601 caccaggccg ccgtcatccg gtagcacgcc gtagccaatc gaatccactt taccctcgaa
     9661 ggttttgccg ctgtcgctca tcaggcgaat cgttgcgggt gtacctgagc gaatattttt
     9721 cagatcggtt tcgcggaagt tggcgatcac gtaccagtga cgagtgtcga ttagggtaaa
     9781 aatagggcgc atggcagaag caaattgtcc gacggaggtt ttgagggaaa tgacccggcc
     9841 atcaaacggc gcgcgaacgg tcgccatttc cagatgcagt ttggtcaggg caatatccgc
     9901 ttcgaccgcc gcacgttggg cgaccagcgc atcgacgccg ctgacggcac tggcggctga
     9961 ctgcgcctgc aacaatacgg cattaagatc ggcttctgcg gcgcgctgcg cagttcttgc
    10021 acggtcaacg tcttccgctg agacaaaacc ttctttcagt aatggctcgg tgcggcgtaa
    10081 tgtatctgtg gcctgtttcg cggcggcacg ggctttttct accgtggcat taaccgagtc
    10141 ggcaccaaac tgttgcgcgt caacgctacg ttgggtgagc ataatttgct tatccagcgc
    10201 cgcgagggag gcttcggctt tcgccagatt ggcttcgtac gggcgcgggt cgatgcggaa
    10261 cagcaaatcg ccctgtttca ccgcctggtt gtcggtgacc gccagttcta caatgcgacc
    10321 gctgacttcc ggcaccacat caatggtatc tgctgacgcg taggcgtcat tagttgatgg
    10381 cgcactgtct acgcgccaga taacgaaaac aagggcaacc agcgccaacg caaccactaa
    10441 cagagcaggg aatttactgc gaggagcttt tttcggcgta ctttccatga gcgcgtctca
    10501 aatagattag aaaaatgcca gccagaacag catggcgtag agagcaaaaa gggcggtata
    10561 aataattccg acaaatgcca gattgatatt tgccctctga ataattcgcc tcgtgatgag
    10621 cgtcaaaata aggctggcaa tggcgcagaa aaaccagctg ggataataag cgccgatcac
    10681 cgggattgcc ggagataatg agcatcccga aaccatgaag acgggaatta tccaggctgt
    10741 ttttttgagt tgcatcgcca tgcgagagag aactgttggc atattaaata catcctgtct
    10801 gctgcgaaca atatctgtag tgaatatcaa tatccattga aggcgtcagt atggctattt
    10861 tctcgatgaa taaaaatagc tttattcagc caccgcattc ataacttata tattttattt
    10921 attaattgtc tgtatatatt tttaaaataa atgcttttgg gggcttcagc gcccccaaaa
    10981 gcgagtggca ttaatttggg gttacgatat tcacccacaa atcgaaatta tccagacagg
    11041 caataatttc ttccagtttt gcgccattgc caataatttt ggctttcttc gcctttacca
    11101 gatccgccat tttggcttgt ccgaccagca cgtcgtgcag atcgctgcgg ctcatgtaaa
    11161 acgatgcgtc taccttcggt tgcagtactt tacggtagtt caatacgcta ttgttgagtg
    11221 agaggttcag gttatcgttg tcagagagat tgaagttcag gctgatggat ttgcccgcgg
    11281 ctttctcact gttcagacga acagccatgt aatcaagcag catctcgacg gtcataccct
    11341 tgatggtgtc aggagaggcg gtgctggcgt gttcgatttt cttcgcgccc tcacgcagtt
    11401 ctttcgctcc cgtcaggtag aagccgcgcc aggtggctga ttcggcctga taacccaatt
    11461 gttcgaaggt atcggcctgt aggtttttcg ccacctggtc tcccggattg gcagcaatca
    11521 cctgtttcag cagttcagag gcccagcggt agtcgccctg gttataggct tcacgcgcca
    11581 gattgatagc atgtgcggaa cctcccagcg ctttgacata acgtttaccc atatcgacct
    11641 ggccatacgg attcaggttt gctgggttac cgtcgtagta gccgaggtaa aagttatata
    11701 ccgcgcgagc gttatgactg acggagccat aatagccacg gctggcccag tttttatcca
    11761 gcgtttccgg cagatgaatc atgttgccga tttcattcat ggtgtaaccc tggttcgcca
    11821 ggtgcagggt ctggtcgtga atatacttaa tggtgtcgcg atattttcca atataatcat
    11881 tgatatgttg attaccccat actggccagg tatgtggcat aaacagcact tctgcttgtg
    11941 atccccactt atccagcgtt tcattcaggt aatcggtcca cttcgcggta tcacgggttt
    12001 tcgcgccacg cagggtgtag aagttatgca gggtatgggt gctgttttcc gctgtgcaca
    12061 gggctttcaa cgccggaata tagaggtgca tttcagacgg cgcttcgctg cctggagcca
    12121 tcaggaattc gaaatccagc ccgtcgatat tgagtttctc tcctgtcttg gtgatagatc
    12181 gcgtcggcgc gataattgtc gggccgccgg tagtaagcgt aacacccagc ccgttgccga
    12241 tatctccctg tgcattatgc gggagtagca ggccgtaaga gtaaaatgca cggcgactca
    12301 tgatattacc cgccagcacg ttttcactga tggcttcgtc cataaagcct gccggggcga
    12361 tgatctgcac tttgcctgct tttacatcgg cttctgagac aatacctttt acgccgccat
    12421 agtggtcagt gtggctgtgg gtgtagataa cggccacaat cggtttttgt gggcggtgtt
    12481 tgaaatagag atcaaggctg gcttttgctg catttggcgt caccagcggg tcaatgacga
    12541 tgatgcccgt atcgccttca atgaaggtga tgttcgagat atcctggctg cgcacctgat
    12601 acatgcggtc ggtgacttta aacaggccag aaatgccgtt gatctgcgac tgacgccaca
    12661 ggcttgggtt aacggtttcg ggcgcgggcg cattgatatc aaatttataa tcttctccgc
    12721 gataatatgg tttgccatta gcatcgcgca gaataccttc atccagcagc ggagcgataa
    12781 atccacgctg agcatcgtca aaatcctgac gatcgctaaa cggtaatgat ttggcgtaat
    12841 tctgattaac ctgttgggta tattgagtgg cggctttcgc ttcatctgtt gcgttagcgg
    12901 tatttaccat catgccgctg gcggcagtta acgcaataac aatcctgctc aaacggaata
    12961 accgagagtt attcatataa atctccattt atcatttatg tcgtaaatat gtaatccatg
    13021 caaaatacgt catttgaagc tggcgttatc ataattggga atttgcgatt ttaataagtg
    13081 gaagtgtgag cggaacgcgc cattttatta ggcatttgtg ctgaagaggg gaaattacag
    13141 tagaactatc attgctgaaa ataatatgag ctgttttatg tgtttattat atatattgta
    13201 cttgtcgttg gcacagcggg acattgcgtt acggtgccaa cgctttactc atggggcttt
    13261 agcacaggac tgtggcaaaa aacgttgatg tgccaatatt gctgcaccct gagcgccatt
    13321 aaagtcagaa gatgaggcgg caataaagcg cacgacctga tacggcagtg gacggcgcag
    13381 gtacttttgg gtcatggcaa tgagagtctc gcgtgggaag gtgggcatat ccatcacgcc
    13441 accacccaga attaccgcat cgggatcgaa cagattaatg ctggtggcaa tggctcgtgc
    13501 cgcgttttca agcagactct ggacgaaagg ggcgttttcc gcatggacga aaagagcgct
    13561 caatgggtaa tttcggggct gttgttcgta ccagcgtctt agcgccattc cagagcaatt
    13621 ggtttccagg cacccaggat tgccacacgc gcagtgttgg gtcatatctc ccagggggat
    13681 atggcccagt tcgcctgcca caccgtgtgc acccgtccac ggcgcaccgt tcatccacac
    13741 tgcgaacccc atccccgtac cgagataggc cgccagaacc agttgttgcg taaggctgtt
    13801 ttctactacg tcccaggaga gttgcaggtt aacgtcgcgg gaaaactcaa ccggacaatt
    13861 cagcgtattt tcgagcttat cggcgagatc atataaatcc gccgctgtta acggcaggtt
    13921 aggcgtagaa ataatggtgc gtttatcttt actgaccagc gccggaaatc ccatcaccag
    13981 accacgacag cgagcgttaa agcgcctgag ttgctcgtca atcatttcgc cgatacccga
    14041 caccaggtcg ggagcaataa cttctgcggt ccgctttttt tcgcagtgta gcgtttcacc
    14101 ttctgctgtc cgcagacaaa agcggatatg cgttgccccc atatccacgc ccgctacgac
    14161 gttatgctgt ttttgcatga ggctgtacct cgctttttgc agccagaatc tgcgcggtca
    14221 taattctcca tgcttcgtcg atattttccg catgattaaa caggccggaa gtgccgacta
    14281 taaagacatc cgcccctgcc gccattagtt tttcgtaagt tgcctggttg caggaaccgt
    14341 ccacctcaat ttcgtactcc agaccttctc gttcacgcca tgccttcagt tcggcaagtt
    14401 tatccagcat ttcaggaatg aacggttgtc cggcaaaacc gggatcgaca gtcatgaccg
    14461 taattttatc ggccttatgg atatagtatt tcatggcctc aactggcgtc tccgggttaa
    14521 ggattagccc cactttcatg tcatgacggc ggatttcatc aatcaggcgg aatgcctgac
    14581 cgttgatagt ttccggatgc aaagtgatga aatcagctcc cgcacgcgcc agttgcgcaa
    14641 tgtaatcctg cggacgcgtt accatcagat ggcagtcgag cggtttactc gccagttttt
    14701 ttacctggct tacgaagaac ggtgagagcg tcagattcgg cacaaagtga ccgtccatga
    14761 tatcgatgtg aaagtaatcg gcatggctgt cgataaattc gatctgttct ttaaatttca
    14821 gcaaatccat acacattaac gagggggaga ttttcatgtt caattcctta cttactgata
    14881 agacggtcaa gggcgacagc cgcgataatt aatccgccca tcaccaccag ttggtaatag
    14941 gtttgtacct gcaaaatatt cagaccgttg ttgatggtgc cgatgatcaa cccgccaatc
    15001 accacagaga aaatgcgccc cttgccgccg aagaaactgg tgccgccaat gatggcgctg
    15061 gcaatggcat aggtttcaaa acccataccg gcaagcggtt ctgcggcacc gagtcgtgca
    15121 gttgagacga cgcctgccag acctgcacaa acaccggaga tgataaacac caccaggatg
    15181 tggaatttca cgtcaatccc ggaatagaac gccgagtttt tgttgccgcc cagtgcgtag
    15241 atgttgcgtc cgagccgcat acgcgttgtc agaaaccaaa ggatgagcgc gacaattagc
    15301 gagaagataa cgggaacagg tatcccaatt acgctggcgg caaagaagtt cacgaagtca
    15361 aatgagaagc cgtataccga gttggcatcg gagatcacca gcgtgatccc acggaaaatg
    15421 gcgttggtac caagggtgat aataaatggg tgtagccccg tccagttgac caggcagccg
    15481 ttgatcgccc ccagtgcgcc gccaaccagt acaccgccaa tcagcgccgc gagaaacggg
    15541 tcaacacctg ccaacatcag tttggcggtc accataccgg aaagcgccag aatcgcgcca
    15601 accgagaggt cgataccagc gaccaggata gcgaaaaact cgcccatgcc gatcaatacc
    15661 gtcacggagc tttgcacaaa aatctgggta atattattgg tggtcagaaa atattctggt
    15721 gacagcgaac caaagatggc gacgatgatc gccaggataa aaaaggtgcc gtatttatcc
    15781 cagaacagcg caaagttgaa cggtttcttc tcgctcgctt cgctttttac tcttgtggta
    15841 aagcccatgc cataatctcc tcttcgctca tgtcatcgcg attcgtcagg atttgcgtca
    15901 gtcgtccttc gcagaacacg gcgatgcggt cgcagacggt gataatttca ggtagttcag
    15961 atgacaccat caggatgact tttccgtcgt ccgccagttg gcgcatcact ttgtaaattt
    16021 cggctttcgc gccaacgtcg atgccgcggg taggttcatc gaaaatgatc acttccgggc
    16081 aacagcacag ccatttggag atcaggactt tctgctgatt ccccccggag agttcggtga
    16141 tattctggtt tatcgaatga catttcagcg ccagcagttc gcgttgattt tcagcggtac
    16201 gttgctcgtc aacttcatga aacaagccca tcgcgccttt atagccgccg tctttcagac
    16261 tgcggctgat cgccatgttc tgagcgatgg aaaagttggg gaaaaaaccg ttatcccggc
    16321 ggctttcagt gatgtaagcc atcccttttt tcacggcatc caggggtgag cgtggagaga
    16381 tatctttgtc attaagacgg atttctccgc cagcgcgttt atcaacgcca aacaggcagt
    16441 tcatcagttc agtacgtccg gaaccgacca gtccggcaaa gcctaatatt tctccccggc
    16501 agacgctaaa tgagatatcc cggacctttt ttctgtcacg gctggtgacg ttccgcacct
    16561 caaaaaccgt ttcgtgcgca aggttgctga cattctcctt catcgcgtta aaacggtttt
    16621 gcagttcgcg gcctaccatc agacggacga tatcgtcatt tgacacatcg ctcaccatgc
    16681 cgctgcaaac gctgctgccg tctttcatca ccgtatagcg gtcgcaaata cggcgaattt
    16741 ccgccaattt atgcgagata tagacgatag ccgtaccctc cttacgcaac tgattcatga
    16801 tcagaaacag atagtccacc tctttattgg tgagtgagga ggtgggttca tccatgatga
    16861 tgactttggc gtcgagcatc agcgttttgg caatttccag catctgcttg tggctgatag
    16921 ataagttcgc cactttctca tctaaatcaa ctttcaagcc cacgcgtaat aacatcatgg
    16981 cggcgcggac acgcatttct cgccagtcga taatattgac accgcagatt tttttcgtca
    17041 gatgacgacc aatatataaa ttttccagta cggttaattc atcaataacg ctgagttcct
    17101 gataaataat cccgataccg agttgtgccg ctaatttatg atccagtttg ttatagttga
    17161 tgttattaat ggtaatggtg cctttggtcg gctcatgtat tccggataac actttcatta
    17221 gcgtagattt acctgcacca ttttctccta gtaatgcatg tatttcacca ggataaaccg
    17281 ttaaattaac cgactttaat gcgtgaaccg gaccaaagga cttgccgatc cccgccatcg
    17341 atatatatgg cgtggccata aataacctct ctgcacgaaa tatcgtatta ttgcctgatg
    17401 ggctacgttt atcaggtcta cgttcaggtt attttgttgg ccggataaga cacttcatta
    17461 gcatcaccat ccggcatcat tcagttttat tgagtgacca ggattgaatc gaccagttta
    17521 aattccggtg ctttatccag cgggataacc ttgccggatt tctcagcgtc aaccatcagc
    17581 ttcagacccg ttgcaccgat atccgccggg ttctgggcaa ccgtcgcggt catttgtccg
    17641 gtttccacca ttttgcgggc ttccggaatg ccgtcagtac cgacgaccaa cactttgccc
    17701 gtttttccgg cgtttgcgac tgcctgagca acacccattg ccatcgtgtc attcgcgcaa
    17761 tagatcgctt taatattcgg attacgttgc aacacgttag tggcgacatc cagtgcttta
    17821 atacggtccc agtcggcagg ctggctggcg acaagcttga tctggcttgc ttttttgaag
    17881 gcttcggtgg caccattacg acgcgcttca ccggaggcgt taccggcttt accctcaatg
    17941 attgcgactt cacccccttc ggcacccagc ttgtcaataa tgaatgacgc gcctttcgcc
    18001 ccgacggcaa cattatcggt ggtgacaaaa ccttccacat taccgccagc ttttttcaga
    18061 ttatccatgt cgattttttc atcgagattg accagataaa tgcctttttt ccatgcgcgg
    18121 gcgacaggca tgaccagatt tactgaggat aatggcgcga aggcgatacc tttgtaattt
    18181 ttattactga gatcttcaaa taactgcaat tgagattgaa aatcgccttc tgaaggagag
    18241 gcaaaaatat caacgctgac gcccagcgtt tttgcttcat cttcaatgcc ttttttcata
    18301 tctacccaaa atgggttgga gagtgttttc aatacgacag catattcggc ggcagcaaaa
    18361 gcgctggttg acaacattaa gcccacgagt gtgccactga aatatttcag atatttattc
    18421 ataatcatgt tctcacggta aggggagagg taactccgcg atggcggaat tacctttttc
    18481 ttatttcagc gcgcctggtg agaagaaatc aacaattgcg ccggtttttt gcatattaat
    18541 attagcttgt tcaatgttga gctgggcgac agagacaaaa aacgcgtcca gcaaagttag
    18601 ttgtaatatt cttgccgagg cattacgacc taataacggt gtttccgggg ccggtgagca
    18661 aataatataa tcggccagct tcgctatcgg tgaatggtag ctatgcgtta tacaaataat
    18721 ctttgccccg ttcttttttg ccagttctac agccgctttt acatcactgg ttcgcccgga
    18781 atgggtcacc accagcacca catctccttc ctgtaacaac gaagcagaca tcatcatgat
    18841 gtgagcgtcc ggataggcct ggcagcgtac gccaatgcgt aaaaacttgt gctgtacatc
    18901 agcacagata gcatttgatc ctccggcacc gtacaaatcc cgctgtctgg cctgatagaa
    18961 aaagcgggcg gcacggtgga tctcatcaac gttgacgatc gactgacctt ccataatggt
    19021 gcgtaaagtg atgttaaata ccttattcac cacatcctgc ggcgcttcat caaaagccaa
    19081 ctcggaaggc aatacttgtt ctgactgaga aaaataatct tccagcgcac tgcgtaagtt
    19141 acgaaagccg ctaaacccca gcagctttga taccttaacg atcatcgctt cagataccgc
    19201 cagagcttct gcgacatctt taattgcggg tgcacaactc aggttaccgg gtttgagtaa
    19261 ccactccacg atgcggcttt cgttttctgt cattccttcc tgcttcattc gcaggtaagg
    19321 cgctaacccg ataccgttcg gaagcgctga atcaaactct gactggctca tcacgttctc
    19381 ttgtccttga attgaagacg ccactatatc accacagcaa gaacagaaaa gatgctggca
    19441 acttcacaaa aaaataaaat ttataaagtt tgttttgttg tgaataatgg agagtggctt
    19501 aaaatttcat ttcatttaat cactttaaaa acatgatgtt atttgttttt ctggtttatt
    19561 ggatgataga tgttgctgga tagtgagtgt ctttaacgct attgcgctgc cagatgtaac
    19621 tctgtaaaac agatagggaa ggcgggtttc atcgattttg ggtgttttta attcatttac
    19681 atcacaaatg ttttttgatt gtgaagtttt gcgcgggcgg ggaagatgaa tgaaaaagat
    19741 tgcatttggc tgtgatcatg tcggtttcat tttaaaacat gaaatagtgg cacatttagt
    19801 tgagcgtggc gttgaagtga ttgataaagg aacctggtcg tcagagcgta ctgattatcc
    19861 gcattacgcc agtcaagtcg cactggtcgt tgctggcgga gaggctgatg gcgggatttt
    19921 gatttgtggc actggcgtcg gtatttcgat agcggcgaac aagtttgccg gaattcgcgc
    19981 cgtcgtttgt agcgaacctt attccgcgca actttcgcgg cagcataacg ataccaacgt
    20041 gctggctttt ggttcacgag tggttggcct cgaactggca aaaatgattg tcgatgcgtg
    20101 gctgggcgca cagtacgaag gcggtcgtca tcaacaacgc gtggaggcga ttacggcaat
    20161 agagcagcgg agaaattgag attcatccac tacttgcatg gatgagtaat gactaatgtg
    20221 gatagagttt ctttttgagg ttggctaatg aaacgctttc cgctatttct tttattcacc
    20281 ctgttcacgc tgtccaccgt tccggcccag gccgatatta tcgatgacac cattggcaat
    20341 attcagcagg cgattaacgc cgcttataac cctgaccgtg ggcgagacta tgaagattcg
    20401 cgcgacgacg gctggcagcg tgaagtgaac gacgatcggc gcagacaata tgacgatcgc
    20461 cgccgccagt ttgaagatcg ccgcaggcag ctggacgatc gccagcgcca gctggatcag
    20521 gagcgtcggc agctagaaga tgaagagcgg agaatggaag atgagtatgg gcgttgatgt
    20581 tgagtttaga gcaggcatcg cccctcaccc atcacgccac cccaatctcc atcccatcaa
    20641 accccgcctc aaaccccgac ggcagtgcgt tttccatcag ccacgcatca aactggtggt
    20701 tgatatgggt cagaatcacc cgtggcgagc ggataacctg attcagcgca agcacggtat
    20761 ttaaatcaca gtgattacgc ggtgcatcct cgcgcggcgg gtggctgcaa tcaatcacca
    20821 ttacctgcgg ctgattattg cgtaaaaatt tcagcgtttt ttccggcaag ccagcggtgt
    20881 cagacagcca cgccacccgg ctgtgtgccg tttccagcag ataaccgaag gtcagttttg
    20941 agtggttgag cggcaggggc gtgacctgta acccttgtaa atcaaacacc acaaacggtt
    21001 ccatcgtgtg gctgaaatca agcaggcccg gatgtttaaa cagatcgtcg cagccctgtt
    21061 catccggcgg gccgtagacc gggatctgat cgcccacgcc ccagcgcagc ggaaacagcc
    21121 cctggacgtg atccatatga taatgcgtca gcaaaaactg ctggaacgat cctggcgacc
    21181 agcgatcggc gagatcgtgc agcccggcgt cgatcagggt gattgcgtcg ttaaacttta
    21241 ctacgccgct gcatggctgg cggcgatact gcggcgagcg tcgtgctctg gcgcaggccg
    21301 cgcactcgca gccccatgcc ggaacgccct gtgcgccgcc ggtgccggtg agcgtgaggg
    21361 tcaggctcat gttacagcgc cttggtgaag cggaagtggc tctgctcgta gccttcgcgc
    21421 agatagaaac ggtgcgcgtc gtggcgcttc acgttggtgg aaagctcagt catttcggcc
    21481 ccggcctggc gggcttcttc ttctgcccat gccagtaatt tgctgccgac attcagaccg
    21541 cgcgcctgcg gcatcaccac cagttcctga atttcgccga tccagttgac atgatgcaga
    21601 tgaaactgca aatgcaggcc gatcatgccg acaacttcgc catccagcag cgccagatgg
    21661 tagcgcatgt tcggatcgcg cagattggcg ttaaacccca cgcgaaacgc gtggtggtca
    21721 aactccgcct gttttagctc acaaatcagc gcgtaaactg cgtcggtgtc gtactgcgta
    21781 gccgggcgaa gctcacaagc aggcatggtg tttctccttc tgatgaatca gcgtcagcag
    21841 cttgtcgacc gactggcgca ggctgccgtc attattgagc gtaagacaat cctgaggagt
    21901 atagcgggca gcgcgcgcca gccgggcgtt aatttcactg gcattttcac gaccacggtt
    21961 ttccaggcgc tggcgcagga tctctggcga aacctgtaaa caaacgggca gcagcgccga
    22021 ttgatagcgc gcccgcgcct gcggcagatg ggcgcgtgag ccgttgacca ccacgtcaaa
    22081 tccagcgtgc agccagaggt caatctcgac gccgacgcca taatacagac cgttagcgtg
    22141 ccagcttaag gccagaaggt tctgccccgc gcgggtaaaa aactcctgct cgctcagggc
    22201 gatatggttt tcacttccgg cgctggcggc gcgcgtgatg tagcgatgcg ctaccagtag
    22261 ctgagtttgt tcccgctggc ggagttccgt caacagactg tcttttccgg agccagaggc
    22321 ccccattaac caaatcagtt ttcccgtcat cagaacaccc ttttaccctg acgccagacg
    22381 tggtcgatat gaatgtggtt gcccttgcga tgcgccagca ccaggtcggc gcgtttgccc
    22441 tcgccaatca ccccgcgatc ctgaagatta agcgcctgcg ccggattttt agtcaccagc
    22501 ttcaccgcct gcggcagcgt aaagcggttg ctctcgtcat cggcgacgcg aaatgccgca
    22561 tcgagcaggc tggcggggta gtagtcggaa gagaggatat ccagcaggcc aagctgcgcc
    22621 agttcactgg ccgccacgtt gccggagtgc gaaccgccgc gcacgatatt cggcgcgccc
    22681 atcagcacgt tcatgccatg cttgcgcgag gcttctgccg cttcgaacgt ggtgggaaat
    22741 tcggcgatca cgctgccaag ctggtgggat tcggcaacgt gggcgtgcgt ggcgtcatcg
    22801 tggctggcga gggcaatgtg tcgcgcgcgg cacattgcgg cgatagtttc gcgattaggc
    22861 tgtgaccagc gtgcggcaag cgccagctgt tcctcttcgt actgctgcat ctgcgcatcg
    22921 gtgagggaat atttgccctg ataatattcg cgatacttct cgcgattggc gaactggcgc
    22981 tggcccggcg agtgatccat cagcgacacc agcgtcaccg gttcgcgctg caccagtttt
    23041 tcaaacagcg gcagcgtggt gtgatgcggc agttcgcagc gcagatgcag acggtgctcg
    23101 gcgcggttga cgccgcgttt ctgcgtctct tcgatggcgt tgatcatctt ctccagattc
    23161 tccagccgat cgccgccgtc gcgcacgtcg ccaatcgcca ccgcgtccag cacggtggtg
    23221 atgccgctcg ccaccatcag cgcgtcgtgg ctactcatcg ccgagtgggc gggccagtca
    23281 accttcgggc gcggggtgaa gaatttatcc agattatcgg tatgcagctc aatcagcccc
    23341 ggcagcagcc agccgccttc gccatccatc gcttctggca ggcggctctg gctttcggca
    23401 aaggcgcgga tttcgccatc ctgcacctcc agcgaaccgt gcaccacctc gttttccagc
    23461 accagcttaa cgttattgat aatcatgaag aggctcccat tgggtgcagg cggtcggcga
    23521 cgtcattacg tacagcttcg tcatggaaga tgcctacgat ggctgcgcca cgggttttgg
    23581 cttcgcgaat cagttccacc accgcggcgc tgtttttggc gtccagcgag gcggtaggtt
    23641 cgtcaagcag cagaatgggg tagtcgacga taaagccgcg ggcgatgttg acgcgctgct
    23701 gttcgccacc ggaaaatgtc gatggtgcca ggtgccacag gcgttccggc acgttcaggc
    23761 gggtgagaag acgcgcggct ttagcggcgc aggcttcacg cggaacgccg gtatcgagca
    23821 gcggctgcat caccacttcc agtgctgaga tacgcgggat gacgcgcaga aactggctca
    23881 cccagccgac ggtggttttg cggatttcca ccactttgcg cgctggcgcg gtgaccaggt
    23941 ctacccactc gtcaccgtgt ttgatctgga tttgaccttc gtcgggtaga tagttggcgt
    24001 acagcgagcg cagcagggtt gatttgccgc tgccggagtg accgtggagc accacgcatt
    24061 cgcctgcgtt gacggtgagc gaggcacgtt gtaagacggg caggcgtacg ccgttttgct
    24121 gatgcaggat gaaggtttta ctgacgtttt gtacgttaat catttttcgc ctcgtggttc
    24181 gggtgtcgga tgcgacgctg acgcgtctta tccgacctac ggggagcgca tttgtaggcc
    24241 agataaggcg tttacgccgc atccggcacc ggctcaattc tgcaaaaccg atgacaccag
    24301 cagctgggta tacggatgat gcgggtcgtc gagcacgcgg tcggttaacc cactctccac
    24361 cacctgcccc tgcttcatca ccagcaaacg gtccgccagc aggcgggcga cgcctaaatc
    24421 gtgggtgaca atcaccaccg ccaggttcag ctccaccacc aggccgcgca gcaggtcgag
    24481 caggcgcgcc tgcaccgaca catccagccc gccggttggc tcgtccataa acaccagctt
    24541 cggatgcgtc accaggttgc gggcaatctg caaacgctgc tgcatgccgc cggaaaaggt
    24601 ggtcggcagg tcgtcgatcc ggttagcggg aatctccacc tcttccagcc acttctgcgc
    24661 ggtggcgcgg atgtcgccgt aatgacgcgc cccggtcgcc atcagccgct cgccgatatt
    24721 gccgcctgcc gatacctggc ggcgcaggcc gtcgagcgga tgctgatgta ccacgcccca
    24781 ttcggtgcgc agtaaacggc ggcggtcggc ttcgctcatg ccatagaggg agcggttttc
    24841 atagcgaatc tccccctgct gcggcgtcag gcgcgcggag atcgacttca gcagcgtggt
    24901 cttcccggag ccggattccc cgacgatgcc cagcacttcc cccggccata aatcaaaaga
    24961 gacatcgcta aagcctttgc ccggcgcgta aaggtgggtc aggttattga ccgaaagtaa
    25021 cggttgattc attggttttt tgcctcgctc tgttggcggc aataattggt atcggagcag
    25081 acaaacatgc ggtttccggc gtcatccagt accacttcgt cgagatagct gtgggtcgat
    25141 ccgcagatgg cgcatggctc attccactgc tgaacggtga acgggtgatc gtcgaaatcg
    25201 aggctttcca cgcgggtaaa cggcggcacc gcatagatgc gcttctcgcg ccccgcgccg
    25261 aacagttgca gggcgggcat catgtccatt tttgggttat cgaatttcgg gatcggcgac
    25321 gggtccatta cgtagcgccc gtttaccttc accggatagg cgtaggtggt ggcgatatga
    25381 ccgaagcggg cgatatcttc atacagtttc acctgcatca cgccgtactc ctccagcgcg
    25441 tgcatggtgc gggtctccgt ttcgcgcggc tcgataaagc gcagcggttc ggggatcggc
    25501 acctggaaaa taatgatctg atcttcggtc agcggcgttt cggggatgcg gtggcgcgtc
    25561 tggataagcg tcgcatcgtc cgtacgttca gtggtgttta ccccggtcac gcgcttaaag
    25621 aagttacgga tcgacaccgc gttggtggtg tcatccgcac cctggtcaat caccttcaac
    25681 acgtcgcttt cgccaatcac gctggcggta agctggatac caccagttcc ccagccgtac
    25741 ggcatcggca tctcgcgccc gccaaacggc acctgataac cggggatcgc caccgctttt
    25801 aagatggcgc ggcggatcat gcgtttggtc tgctcgtcga ggtaggcaaa gttgtagccg
    25861 ctcagattag ccatggttcc gctcctgttg cagacgtttg agtagctcca gttcggcctg
    25921 gaaatcgacg tagtgcggga gtttaaggtg cgagacaaag cctgcggctt cgacgttgtc
    25981 ggcatgcgcc agcacgaact cttcatcctg cgccgggcct gtcgcgtgct cgccgtactc
    26041 cggggcttgt agagcgcggt caaccagcgc catcgccatc gctttgcgct cgctcatgcc
    26101 gaacaccagc ccgtagccgc gcgtgaagtg cggcggctca tccggcgggt cgataaaacc
    26161 gttgaccatt tcacattcgg tcatcagcag ttcgccgacg ttcaccgcaa atcccagctc
    26221 ttccggcaca atcgacacgt cgatatagcc gctgcggatt tcgcctgcga acggatgatt
    26281 gcgtccgtaa ccacgctggg tggagtaggc cagcgccagc aaatagcctt cgtcgccgcg
    26341 cattaattgt tgcaggcggg aggagcgtga gcaggggtaa accggcggcg tgcgggtgat
    26401 gtcgtccggc tgtgcgccgc tatcttcttc aaacttcgcc agcccctgac gcgccagcag
    26461 gctgaaaacg tgcggcgacg gctgttgttc gctgtcggct gtggtcagcg tcggcgcttc
    26521 gccgtttgcc agcagggtga aatcgagcag gcgatgggtg tagtcgtagg ttgggccaag
    26581 cagctggccg ccgggaatat ctttataaac cgctgaaata cgccgttcga gacgcatctc
    26641 cgtggtgtcg agcggctcgc tcaccgcaag cttcgccagc gtggtgcggt aggcgcgtag
    26701 caggaaaatc gcttcgacgt tatcgccgct ggcctgtttc agcgccagcg ccgccagttc
    26761 gcggtcggca atgccgcctt cggtcatcac gcgatctact gcaagattaa gctgctgttc
    26821 aatctgggcg acgctcagtt caggcaaatc ggtattgccc cggcgtcggc tctcttgcag
    26881 ggcgtgggcg gcgtcgatcg ccttctcgcc ccctttcacg gcaacgtaca tcagcacacc
    26941 tccacatgag tggttcgcgg gatagccagc aggcgctcgc cgcaggtcag gatcaggtcg
    27001 atgccgagcg ggaacgggtg cgggcgctcg gtaagttcgt gcagaatgca ctccggcagc
    27061 tgcggggcga tcattcgttc ttcggcaata cccgcgccag taaggcgcag catgcgcccg
    27121 ccgctcaggc tgacgacctg taaaatcagc gtcgcgcccg cttctggcgc aacagcggtg
    27181 ccgctggaaa gggcgttaag ctgttcgctg gaaatcgcct catccgttac cgcgaaggtc
    27241 gccagttccg gctgattgac cagcggcgcg ttggtatgaa aacgcaggct ctggctgacg
    27301 atatcgttac ttaatggcgc agaaagccac accggcgtgt cgttatcggc cagcgtcagc
    27361 agcacgctgg tggtggcgat attcagcggc tgccagccgc gtttgagctg atgcagggcg
    27421 acaatcacgc ccggctcgct catggccttt aacaggcgac gaaaactgtg ctgggcatcc
    27481 tgcacgggaa gcataaaagc ggtttccagg gtcatgcgtt atctccgcga accatcgtaa
    27541 agaagtcgac ccggctggcg tttacttcgg cctggcgtgc ggcaatgcgt gccatacggt
    27601 cagcgttcag cggggcaata agagtttctg ataagttttg aaaatggcgg gtttgctgca
    27661 tcagcgcgtc gatcagcgcg cagcgttcgg cgtgctgttt gtcgcgcccc agcacccagc
    27721 tgtagccgag cgtgccgtca gtcaggcgta cggcggcgcg ggtgagcgtg gcgtcgccag
    27781 caaaaaagcg ttcgccggtg ccacccatcc gcgcctgaat ctgcaccagg ccggtttcag
    27841 cggcgcggat tacttcatag tcggcggtga tgttcagcgc gttcaggcgg gctgccagct
    27901 cagtcggttg gctgtgcgcc agtacggaca tccagtgctg gcgggtcgcg gtatctgcgt
    27961 gcattcagtg ctccatagtg aattcaatca tgtcggcgcg cgtcaggctg acggagtact
    28021 ccgccgggct gctttcaccg tcacggtggt taagggtgcg cacgcacagc agcggcgaca
    28081 tattcgggat ttcaagacgc tggcactctt tggcctgggc gcggcgggcg ctgatccgcg
    28141 tctggctgcg gcgcagcgca attccggttt gctcgcgcag aaaatcgtgc agcgagccgc
    28201 tgtcgaagcg ttgcagcgtc ggccagaggg cgaggtccgc aaagtagtgg tcgattaaac
    28261 agagcgcgac gccgttgacc cgacgcaggg tgcgcaggtg gataacgttc tcgccctcgg
    28321 taatccccaa tgcgtcagcg acgtgaccgg aggcggggcg cagcaccgat agcaattttt
    28381 cgctggtggg atggctgcct tggtccagca ggttctggct aaaacgcgcc tgggcgttga
    28441 gcgggtaatc gaacgggcgc atcagcacca gcacgccgac gccctgacgg cgctgtaccc
    28501 aacctttttc caccagttgg tcgatggcgc ggcgcagggt gtggcggttc acctcaaagc
    28561 gcgccgccag ttgctgctcg gcgggaagat agtcgccgca gcggtagtgt tgacgaagct
    28621 cctgctcaag ttttgcggct atctcctgat agcgtgttgg gtagctggtc ggatgtgtag
    28681 acaagtgcat agatatcaat gcctcgctta tcagataaag tgcttacgca accgttgaga
    28741 gaggaaatcc agcaggctga ccgtgacgat aatcagcacc atcagggcgc aggtttgctg
    28801 gaactggaaa ccgcgaatcg cttcccacag ggtgacgccg atcccgcctg caccgaccat
    28861 gccgacgacg gtcgccgagc ggacgttgga ttcgaaacga tagagggagt aggagatcag
    28921 cagcggcatc acctgtggca gcacgccgta gaggatctct tccagcttgt tggcaccggt
    28981 ggcgcgaatg ccctccaccg ggccgggttc gatcgcttct accgcttcgg aaagcagctt
    29041 ggagagcacg ccggtggtgt ggataaacag cgccagcacg ccagcgaacg ggccaaggcc
    29101 gacggcgacc acgaacagca tggcgaagac catttcgtta atggcgcggc aggcgtccat
    29161 caggcggcgt acgggctggt aaacccacca cggcaccagg ttttcggcgc tcatcagacc
    29221 gaaggggatg gagagaacca ccgccagcgc ggtgccccag acggcgattt gcagcgtgac
    29281 ggccatttcg gtgaggtaat cctgccactg gctgaaatcg ggcgggaaga agtcggcagc
    29341 gaacgtcgcc atgttgccgc cgtctttgat cagcgtaagc ggggccattt ccgcgccctg
    29401 ccacgagacg accaacacgg cgagaacaat ggcccagctc agaagcgaga accagctgcg
    29461 cttgggtggg cgatggtgat ggtttgcatg tttggctccg tgattgtgtg ctcgatcggt
    29521 tccctcgccc cttcggggag agggagaaaa ccattactgc accgctttac ttaccgaact
    29581 catcgcgctt aacgcgttgt tcaggcggtc caggtcatcc agctgcgcct gaatcgcggt
    29641 ggttttcgcc agcttgtcct gctcattcag ccctttattg cttttcacgc cctgcatctc
    29701 tttaaacagt gcgagctggc gaatcggcac cagttgcagg tcgctggagg cgcggaacgg
    29761 ggcccagccc aggcgttcca gcaccgcttt ctcttccggc gtcttgccgt aattcataaa
    29821 gaagtcgtag atcttgtctt tggtagtttc ggaaagattt ttgcgccaga cgatcggatc
    29881 gcctgggatc agcggcgatt tccagatcac cttcagttct ttgagcttct ctggtgcgga
    29941 ggttttcagc ttgtcgaggt tttcggtatt gttggtcgcc acatccactt gcttgttggc
    30001 gacggccagc gcattggttt catgcccggc gttgacggtg cgcttgaagt cgctggcgga
    30061 gatattgttt ttggcaaaga cgtagtaacc ggggacgagg aagccagagg tggagttagg
    30121 atcgccattg ccaaaggtga gatctttacg cttcgccagc agatcgttca ggttgttgat
    30181 cgggctgtct ttgttgacga tcaacacgct ccagtaacct ggcgatccat ccgccgcgac
    30241 tgtctgggcg aagacctgac cgttggcgcg atccaccgct tccatcgccg acaggttgcc
    30301 gtaccaggcg atatccactt tattgaagcg catcccctgg ataatgcccg cgtagtccgg
    30361 ggcaaagaag gcgttgacct ttacgcccag cttcttctcc atatcctgta agaacggcgt
    30421 ccattgcggt ttcaggtttt gctgtgattc cgttgaaata atgccgaaat tcagcgcctt
    30481 ttcctgctct tcggcgtgcg ccgggcttaa cagggtgctg aggctgaaca tgctggtgaa
    30541 ggccagcgag gcaattatct tagcgttcat tcgttttcct caatgatggg gatgtcaggc
    30601 agctttcgcg ttctcttcga cgcggttaat gctgcggtag agatggtcaa aacgttcgtt
    30661 atcaaactgt tggctgctgc cgtcgtagaa gacgtgcccc tggcgcaggg cgacgatgcg
    30721 ttcgcagtag cgcagggcgt aatccacctg atgcagcgtg acgaccacgg taatgccgtc
    30781 gttctggtta atgtcgcgca gggtgtccat cacgatgcgc gccgattccg ggtccagcga
    30841 ggcgatgggt tcatcggcca gaatcacctt cgcctgctgc atcagcgcgc gggcaatcgc
    30901 cacacgctgc tgctggccgc cggagagggt ggaaacgcgc tgatgggcaa aatgcaccat
    30961 gccaacgcgg gtcagcgcct gtaacgcgcg ctgtttctgc tcgcgggtga accagctaaa
    31021 acaggtgcgc cagaacggcg tgctgccgag cgcgccaatc agcacgttct ccagtacgct
    31081 caggcgattc accaggttga attgttggaa gatgtagccg gtgttggcgc ggcttttgcg
    31141 gatatcgcgg gccaggcggc cttcgcgctg gactgtgcgg cccagcagct cgatatggct
    31201 accggcagat ttatcgccgg taatcaaacc gcttaagtga cgtaaaaggg tggatttgcc
    31261 ggaacccgac ggcccaagca gagccaccat ttcaccgtga tgaatgttca gatcaaccgc
    31321 atgcagcgcc tgatgctgat tgaaggtttt ggcgagcttc tcgacacgga taatcgtttg
    31381 catgatgcag cctccctaaa aaagtggctc catcgtggcg gattattgtg acgattttgt
    31441 taactaaaag tgactaacgg atgaagagtt aacgagattt cgatgacagt gcgggagggc
    31501 ggccctcccg gcgggttagt gcgttggttg ttgtttgacg acattaatca tccacggtac
    31561 gccaaattta tcggtgactt tgccgaagcc atgcgcccag aaagtttcct gccaggccat
    31621 ttcgattttt ccgttagcgg caaggttgtc aaaccagcgt tttccttctt cgacctgttg
    31681 cgaatcgagc accagcgtaa agccggagta gctggctttt cctgacggta tagcatcgct
    31741 catcatgatg tcgcttcctg caatgcgcac gttggcatga gcgatggcgg tatcgggaaa
    31801 ttgcattccg gaagggcagt tctcggcgct atcctgcgct gattttggca tttcgccgaa
    31861 gctgatttta tagagcagtt cagcgcccaa cgtacgttga taataagcaa ttgcgtcggc
    31921 acagttaccg gcaaaagaga ggtagggact taacggcatg atggtgacct cagttaagag
    31981 aagccagtta agtgtagttc gaaatttata cagatgagag ggaagcctga taagacgcgg
    32041 tcagcgtcgc atcaggcagt cggcatcgtt gccggatgcg gcgcgagcgc cttatccggc
    32101 ttaccgatca caatacaatc agttcttttt cacaaactca gatttcagtt tcatcggacc
    32161 aaaaccgtcg attttgcaat cgatgttatg gtcgccttca accaggcgga tgtttttcac
    32221 tttggtgcca attttcagca tcgaagagct acctttcacc ttcagatctt taatgatggt
    32281 aacgctgtcg ccgtcagcca gcagattgcc gttagcatct ttaacgatca gctcgtcgct
    32341 ttcctgtgca ggttctgcgt cgttccattc gtaggcacat tccgggcaga tgtacatgcc
    32401 gttatcttcg taggtgtatt cggagttgca ttttgggcag tgtggtaatg acatgtggat
    32461 ttcctcaaaa gtcagcaggg caaaagcgcc aaaaaacggc agattgccga aaaaggccgc
    32521 aattatacac aaaatccctg agtttgtcgg gggtactgct gattttagac gacgatcgtt
    32581 ttcatttgaa aggaaacgca aaaagaaaat aatctttttg cctccattta ttatttaaga
    32641 taaggttaat gctctacttg ttcttttttt aattcaaatg atatttttta tctttctgac
    32701 gttttaaacg tatcggaaga cgttatttat tctaataaaa aataacactc taaatataaa
    32761 attatttctt gcagtaatta taaaagccga aaacagaact caaaagcaat ctggctaaat
    32821 aaaagtaata aaatttgctt taaggaagaa ttttctatgt acacacagac cctgtatgag
    32881 ttaagtcagg aggctgaacg cctgttacag ctttctcgcc aacagttgca gttactggaa
    32941 aaaatgcctc tctctgtacc cggagacgat gcgccacaac tggctttacc ctggagtcag
    33001 cctaatatcg ccgaacgtca cgcgatgctg aataatgagt tgcgtaaaat ttcccgactg
    33061 gaaatggtgc tggcgattgt cggtaccatg aaagcaggga aatcaaccac cattaatgcc
    33121 attgttggta cggaggttct gcctaatcgc aatcgcccaa tgactgcgct gccgacgctt
    33181 attcgccata cgcccgggca aaaagaaccg gtactgcatt tttcacatgt cgcgccaatc
    33241 gattgtttaa ttcaaaaatt acaacagcgc ctgcgtgatt gcgatattaa gcatctgacc
    33301 gatgtgctgg aaatagataa agatatgcgt gcgcttatgc agcggatcga aaatggcgtc
    33361 gctttcgaaa aatattatct gggtgcccag cctatttttc attgtctgaa aagtttgaat
    33421 gatttggtgc gactggcgaa ggcgctggac gtcgattttc ctttttctgc ttacgccgcc
    33481 attgagcata ttcccgtgat tgaagtggag tttgtccatc tggcggggct ggagagttat
    33541 cccggccaat tgacgttact ggataccccc gggccaaatg aagccgggca accgcatctg
    33601 caaaaaatgc ttaaccagca gctggcacgc gcctcggcgg tactggcggt gctggattat
    33661 acgcaactga aatcgatctc cgatgaagag gtccgtgagg cgattttggc ggtggggcaa
    33721 tcggtgccgt tgtatgtgct ggtcaataag ttcgatcaac aggatcgtaa cagtgacgac
    33781 gccgaccagg tgcgggcact gatttccggg acgctgatga aaggctgtat tacgccacag
    33841 cagatatttc cagtgtcgtc gatgtggggc tacctggcga atcgggcgcg taatgagtta
    33901 gccaacagcg gtaagttacc cgcgccagag caacaacgct gggtggaaga ttttgcccat
    33961 gccgcgctcg gcaggcgctg gcgtcatgcc gacctggcga acctcgaaca tattcgtcat
    34021 gctgccgatc agttgtggga agattcgctg ttcgccaagc caattcaggc gttgcttcat
    34081 gccgcttacg ctaacgcctc gttgtatgct ctgcgatctg ccgcgcataa actgttgaat
    34141 tacgcgcagc aggcgcggga atacctggat tttcgtgcgc acgggttaaa cgtcgcttgt
    34201 gaacaattgc ggcaaaatat ccaccagatc gaagaaagtt tgcagctatt tcaactcaat
    34261 caggctcagg tgagcggcga gattaaacat gaaatcgagc tggccctgac ctccgccaac
    34321 ctctttctgc gtcaacagca agatgcggtg aatgcccagt tagccgcgtt gtttcaggat
    34381 gattcggggt cattaagcga gattcgtacc tgctgtgaga cactgttaca gacggcgcag
    34441 aacaccatca gtcgcgactt tacgctgcgt tttgccgagc ttgaatccac cctttgccgg
    34501 gtgttaaccg atgttattcg gcccattgag caacaagtca aaatggaatt gagcgagtca
    34561 gggtttcgtc ctgggtttca ttttcctgtt tttcacagcg cagttcccca cttcaacact
    34621 cgccagctgt tcagtgaagt catttcgcgc caggacgcaa tggacgagca gagcacgcgt
    34681 ttaggcgttg tgcgtgagac tttttcgcgc tggttgaatc agcccgactg gggacgggga
    34741 aatgagaaat ccccgacaga gacggttgat tacagtgtgt tgcaacgagc attaagcgca
    34801 gaagtcgatc tttattgcca acaaatggct aaagttctgg cagagcaggt cgatgaatct
    34861 gttacggcag gcatgaatac ttttttcgct gagttcgctt catgtttgac ggaattacag
    34921 acgcgtttac gcgaaagcct ggctctgcgt caacaaaatg aatcggtggt caggctgatg
    34981 cagcagcaat tgcagcaggc tgtgatgact cacagctgga tttacaccga cgctcagctg
    35041 ttacgcgatg atattcaaac acttttcacg gcagaacgat attgaccaag acgttactgg
    35101 acggccccgg tcgcgtgctg gagtcggttc atccgcgctt tttagtggat ctggcgcagg
    35161 gcgatgatgc ccgccatcca caagcccatc agcagcagtt tcgtgaacga ctgatgcagg
    35221 aacttcttgc gcgtgtgcag cttcaggcat ggacgaacag cgggatgtta aatgcgccgc
    35281 ttagcctgcg tctgacattg gtggaaaaac tggcgtcgat gctggatccc ggtcatctgg
    35341 cgctgacgca gatcgcgcaa catctggcgc tgctgcaaaa aatggatcac cgccagcact
    35401 ctgctttccc tgagctcccc cagcaaattg ccgccttgta tgagtggttt tcagcccgtt
    35461 gtcgctggaa ggaaaaggcg ttaacgcaac gaggcctact ggtgcaggcg ggtgaacaga
    35521 gcgagcaaat ttttacccgc tggcgtgctg gggcgtataa cgcctggtcg ttgcctgggc
    35581 gctgttttat cgttctggag gagttgcgct ggggggcatt tggcgatgcc tgccgtctgg
    35641 gaagcccgca agcggtggcg ttgttgctgg gtgatttgcg cgtgaaagcg acacaacatc
    35701 tggcagagag tatcaatgcg gccccgacca cgcgtcacta ttaccatcag tggtttgcct
    35761 cttcgaccgt ttcgacgggc ggggatcatg ctgatttttt aagttggctg ggaaagtgga
    35821 ccacggcaga taaacaaccc gtttgctggt cagtgaccca acgctggcaa actgtcgcgc
    35881 tggggatgcc acgactctgt tcagcgcagc gtctggcggg ggcaatggtc gaggaaatct
    35941 tctctgtaaa tttggtgtaa ataatcagtt acaccaatga gtcctaaacg aaatacatgt
    36001 gtgaagttga tcacaaattt aaacactggt agggtaaaaa ggtcattaac tgcccaattc
    36061 aggcgtcaac tggtttgatt gtacattcct taaccggagg gtgtaagcaa acccgctacg
    36121 cttgttacag agattgcatc ctgcaattcc cgctcccctt ttgcggccgt cgcgctgatt
    36181 tttctggcgt ttgcggaaat gggccaactc tgcgaggaaa gctatgctga aaaggaaaaa
    36241 agtaaaaccg attacccttc gtgatgtcac tattattgat gacggtaaac tgcgtaaagc
    36301 cattaccgca gcatcactgg gtaatgcaat ggaatggttc gattttggtg tttatggttt
    36361 tgttgcttac gcattaggta aagttttttt cccgggggct gaccccagcg tgcagatggt
    36421 tgctgcactt gccactttct ccgttccctt tctgattcga ccgcttggcg ggctcttctt
    36481 tggtatgttg gtcgataaat atggtcgcca gaagatcctc gctatcacta ttgtgattat
    36541 gtcgatcagt acgttctgta ttggcttaat accgtcctac gacacgattg gtatttgggc
    36601 accgattctg ctgttgatct gtaagatggc acaaggtttc tcggtcggcg gtgaatatac
    36661 cggggcgtcg atatttgttg cggaatactc ccctgaccgt aaacgtggat ttatgggcag
    36721 ctggctggac ttcggttcta ttgccgggtt tgtgctgggt gctggcgtgg tggtgttaat
    36781 ttcgaccatt gtcggcgaag agaacttcct cgactggggc tggcgtattc cgttctttat
    36841 cgctctgccg ttagggatta tcgggcttta cctgcgccat gcgctggaag agactccggc
    36901 gttccagcag catgttgata aactggaaca gggcgaccgc gaaggtttgc aggatggccc
    36961 gaaagtctcg tttaaagaga ttgccactaa atactggcgc agcctgttga catgtattgg
    37021 tctggtaatt gccaccaacg tgacttacta catgttgctg acctatatgc cgagttattt
    37081 gtcgcataac ctgcattact ccgaagacca cggggtgctg attattatcg ccattatgat
    37141 cggtatgctg tttgtccagc cggtgatggg cttgctgagt gaccgttttg gccgtcgtcc
    37201 gtttgtgcta cttggtagtg ttgcactgtt tgtgttggcg atcccggcgt ttattctgat
    37261 taacagtaac gtcatcggcc tgatttttgc cgggttactg atgctggcgg tgatccttaa
    37321 ctgctttacg ggcgttatgg cttctacctt gccagcgatg ttcccgacgc atatccgtta
    37381 cagcgcgctg gcggcggcat ttaatatttc ggtgctggtt gccggtctga cgccaacact
    37441 ggcggcctgg ctggtcgaaa gctcgcagaa tctgatgatg cctgcctatt acctgatggt
    37501 agtggcggtg gttggtttaa tcaccggcgt aaccatgaaa gagacggcaa atcgtccgtt
    37561 gaaaggtgcg acaccggcgg cgtcagatat acaggaagcg aaggaaattc tcgtcgagca
    37621 ttacgataat atcgagcaga aaatcgatga tattgaccac gagattgccg atttgcaggc
    37681 gaaacgtacc cgcctggtgc agcaacatcc gcgaattgat gaataagctg aaacggatgg
    37741 cctgatgtga cgctgtctta tcaggccaat tgaactctta aggttcactt aatctctgac
    37801 gcgcatactc tcctccaggt taacggagga gagtgcaatg aaaaaccgtg tttatgaaag
    37861 tttaactacc gtgttcagcg tgctggtggt cagcagcttt ctttatatct ggtttaccac
    37921 gtactgatct ttcttcagcc gtacccaggc ccgcgtgccg gaagtctctt gccggttttg
    37981 caggaaaaac tgcccgtgat gcaactgtgt aatgcggctg acaatactta accccagacc
    38041 aatcccgcca taacggctgt ccatacgtac aaacgcttta ctcaactccc cgcatttact
    38101 ctcatcaata cccggtcctt catcttcaac ggccatgact gctccgccat cttcttgcag
    38161 cttaatcata atgttgctgc cttgcgggct gtaacgatgg gcgttttcta ccaggtttcg
    38221 caataacatc cgcagcaggg ttgcatcacc ctgaacggtg atgtcggcgg cgctctctgg
    38281 caatagcagg gtttgctgtc gctggtcgag catggtactg agttcgtcat acgaggggag
    38341 aatgacatct tccagcagtt ttacatgttg ataattcccg gaagaaaatg actgtccggc
    38401 acgcgccagt tgcagcagct gggagacact ctccatcatc tgatcaagtc gagccactaa
    38461 cggtgctaca tcaatgtgat gcgttttcgc cagcagttcc agatgcaaac gcacccccgc
    38521 cagtggcgtt cgcagttcgt gcgcgacgtc agcggtaaac aacctttcgt tatccagcgt
    38581 gctggtcagg cgactgacca gatcgtttaa cgctgaaacc accgcttcga tttcgagggt
    38641 ggcgctgtga atggcaatgg gcgttaagtt gtcggcggtg cgcgcttcca gctctttttg
    38701 cagctccgcc agcgggcggg tgatgcggcg taccgcctga tagcagataa atagcgtcag
    38761 gctgaccata aagacgccgg ggacaatcag gctggcgacc gcctcgcgga tctcacgcat
    38821 gatgtggcga tcgttgttgc gattgtcgcg tagcgcctgc tcaaacagct gaatctgctc
    38881 ggtactttca tgccatagcc agaagacgct gatcagctca aacaccaaca aaatggcccc
    38941 gatggtcaat atcagccgtt ggcgcagcga tattggtcgg cgcagaaaac gcatcagatt
    39001 caattagttt tcctcatttg cgaccagcat atagccaaat ccgcgcacgg tgcggatacg
    39061 ggctttgccc actttgtcgc gcagattgtg gatatgcact tccagggtgt tggtcgaggg
    39121 ttcattgtcc cagttata
//
LOCUS       DAMZFT010000030        38327 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.030, whole genome shotgun sequence.
ACCESSION   DAMZFT010000030 DAMZFT010000000
VERSION     DAMZFT010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 38327)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38327)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38327
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.030"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            5..1297
                     /locus_tag="PN977_001695"
     CDS             5..1297
                     /locus_tag="PN977_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011095841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDI6041737.1"
                     /translation="MPPDGLVQRSTRIKRIQTTAMLLLFFAAVINYLDRSSLSVANLT
                     IREELGLSATEIGALLSVFSLAYGIAQLPCGPLLDRKGPRLMLGLGMFFWSLFQAMSG
                     MVHSFTQFVLVRIGMGIGEAPMNPCGVKVINDWFNIKERGRPMGFFNAASTIGVAVSP
                     PILAAMMLVMGWRGMFITIGVLGIFLAIGWYMLYRNREHVELTAVEQAYLNAGSVNAR
                     RDPLSFAEWRSLFRNRTMWGMMLGFSGINYTAWLYLAWLPGYLQTAYNLDLKSTGLMA
                     AIPFLFGAAGMLVNGYVTDWLVKGGMAPIKSRKICIIAGMFCSAAFTLVVPQATTSMT
                     AVLLIGMALFCIHFAGTSCWGLIHVAVASRMTASVGSIQNFASFICASFAPIITGFIV
                     DTTHSFRLALIICGCVTAAGALAYIFLVRQPINDPRKD"
     gene            complement(1346..3010)
                     /gene="tsr"
                     /locus_tag="PN977_001696"
     CDS             complement(1346..3010)
                     /gene="tsr"
                     /locus_tag="PN977_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HDI6041738.1"
                     /translation="MLKRIKIVTSLLLVLAVFGLLQLTSGGLFFNALKNDKENFTVLQ
                     TIRQQQSTLNGSWVALLQTRNTLNRAGIRYMMDQNNIGSGSTVAELMQSASISLKQAE
                     KNWADYEALPRDPRQSTAAAAEIKRNYDIYHNALAELIQLLGAGKINEFFDQPTQGYQ
                     DGFEKQYVAYMEQNDRLYDIAVSDNNASYSQAMWILVGVMIVVLAVIFAVWFGIKASL
                     VAPMNRLIDSIRHIAGGDLVKPIEVDGSNEMGQLAESLRHMQGELMRTVGDVRNGANA
                     IYSGASEIATGNNDLSSRTEQQAASLEETAASMEQLTATVKQNAENARQASHLALSAS
                     ETAQRGGKVVDNVVQTMRDISTSSQKIADIISVIDGIAFQTNILALNAAVEAARAGEQ
                     GRGFAVVAGEVRNLAQRSAQAAREIKSLIEDSVGKVDVGSTLVESAGETMAEIVSAVT
                     RVTDIMGEIASASDEQSRGIDQVGLAVAEMDRVTQQNAALVEESAAAAAALEEQASRL
                     TEAVAVFRIQQQQQHQRETSAVVKTVTPAAPRKMAVADSGENWETF"
     gene            3192..4055
                     /locus_tag="PN977_001697"
     CDS             3192..4055
                     /locus_tag="PN977_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HDI6041739.1"
                     /translation="MAYSIDIISCITERFMALTATEKRIAQFILDDVSAAVELPIAEL
                     ARLTQTSQASVTRFARALGCKDVRELKVKLAQSLAVGQRFILDVPDLDGVQGIYEAII
                     SVLETHRRSLDMNAISQAVSWLSQARQILALGTGGGSTICSQEIQYRLFRLGLPVVSQ
                     NDALMMRMMCSAVMPQDVVIVLSLGGYTPEIIESAAIARQYGARVIAITPAQTPLAEQ
                     VDLVLPLLVQESDYIFKPTPSRYAMLAMVDVLATELAMANKAQAKDRLRRIKLVLDSH
                     RGGANRQPLGD"
     gene            complement(4065..5534)
                     /locus_tag="PN977_001698"
     CDS             complement(4065..5534)
                     /locus_tag="PN977_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001084240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HDI6041740.1"
                     /translation="MNSHIFLVGFIIYAIAMIWLGWFVSRNQKSGEDFLLGGRSLPLF
                     LTLGSTVATMVGTGSSMGAVGFGYSNGWAGMLYGVGGAVGILLVAWLFAPVRKLRFMT
                     MSEEISYYTGGSHLIKNIVGLMIFIASIGWLGAHILGGSMYLAWATGIDLTVAKLIIA
                     LAFAIYVIIGGYSAVVWTDTIQALILFFGFILMAILAVVHVGGWSAIEQAMDPKAMSL
                     FAIDKMGVLPALSLALVIGVGVLATPSYRQRIYSGKDVPSVRRSFVITGVLYLFFSIL
                     PAIIGMAAFTMNPNLENSNYAFLFATSFLPAILGLVVLIAGLSATMSSASSDAIAAVA
                     IMMRDVYTMITGRMPPEDKAITYSRWMLTFVIGLALVFALTSNDIISYITKMISMLMS
                     GLFICSILGRFWLRFNWQGALAALVSGMVMSIVVLMNADWLAYWGNPCIPSVLGSLVA
                     GVLVTLVTPASQVSREEALAIITNERENQSVVITKPEEA"
     gene            complement(5637..6026)
                     /locus_tag="PN977_001699"
     CDS             complement(5637..6026)
                     /locus_tag="PN977_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000023755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HDI6041741.1"
                     /translation="MSIKRYGTEGGTGTGGQHLPFARAVEAGGWLYVSGQTPMKNGEV
                     VEGGIVEQSRLAIQNCVDIMTEAGYSLVDVVHVKVILTDARYFQSFNKVFREFFGENP
                     PARICCVADLVVDCKVEVDVTCYNATR"
     gene            complement(6036..7478)
                     /locus_tag="PN977_001700"
     CDS             complement(6036..7478)
                     /locus_tag="PN977_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004100781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacylase"
                     /protein_id="HDI6041742.1"
                     /translation="MQVDWLIKNVTVIDGSGGPEFRGDVAITGDRIVDIAPALNVTAQ
                     QVIDGEGRVLAPGFIDVHTHDDINVIRIPEYLPKISQGITTVIVGNCGISAASAKMKG
                     EVPDPMNLLGEAEHFIYPTVESYAQAVEAARPSLNVGTLIGHTALRNNHMDDLFRPAT
                     VDEIAAMRADLRLALSQGALGLSSGLAYATAFQATTEEVMALAEELAGEKGVYTTHLR
                     SEFEPILDALDEAFRIGRHGKVPVVVSHHKCAGAKNWGRTKETLAFFDQMRQHQEIGC
                     DVYPYSASSSTLDLKQVTDEFDIVITWSQTHPQQAGKTLAQIAIDWQMSMLEAAKLLM
                     PAGAIYYNMDERDVRRVLSYPVSMIGSDGLPNDPMPHPRLWGAFPRVLGHYCRDEGLF
                     PLTTAIHKMTGLSASRFRLPQRGLVKVGYFADLVLFDPQTIRDVASFSDPKRPADGIE
                     AVMVNGVMSYGPDKHITGRAGRFLRRQTSH"
     gene            7679..8899
                     /locus_tag="PN977_001701"
     CDS             7679..8899
                     /locus_tag="PN977_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid deaminase"
                     /protein_id="HDI6041743.1"
                     /translation="MKYHSEPLVPHKSALMQMPANLLAEDVCLPAAIIKKQALENNIT
                     WMQRYADARGVSLAPHGKTTMTPWIFQAQQAAGAWAIGVGSAWQAGAAMASGIQRVLM
                     VNQLVGKANMQLIAQLQRHYPTVDFISCIDSIENARALSAFFASQQQTLNVMIELGVP
                     GGRCGCRSRDAALTLAKQAAQLPGLRLRGLELYEGVLHGDDPQPQVEALLRDAAQLAC
                     DMASLVDGEFILTGAGTVWYDVVCNIWLAAEKPDNCRIVIRPGCYITHDTGIYDAAQQ
                     QLLARDPVACDLAGDLTSALELVAMVQSVPEADRAVVNFGKRDCAFDAGLPQPVAHYR
                     NGKSLAFDPQAIRSTGIMDQHCMLQLGADSDVQVGDILVFGTSHPCLTFDKWKTLLLT
                     DDDYNVLAELDTLF"
     gene            9167..11317
                     /gene="btsT"
                     /locus_tag="PN977_001702"
     CDS             9167..11317
                     /gene="btsT"
                     /locus_tag="PN977_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate/proton symporter BtsT"
                     /protein_id="HDI6041744.1"
                     /translation="MDTKKIFKHIPWVILGIIGAFCLAVVALRRGEHVSALWIVVASV
                     SVYLVAYRYYSLYIAQKVMKLDPTRATPAVINNDGLNYVPTNRYVLFGHHFAAIAGAG
                     PLVGPVLAAQMGYLPGTLWLLAGVVLAGAVQDFMVLFISSRRNGASLGEMIKEEMGPV
                     PGTIALFGCFLIMIIILAVLALIVVKALAESPWGVFTVCSTVPIALFMGIYMRFIRPG
                     RVGEVSVIGIVLLVASIYFGGVIAHDPYWGPALTFKDTTITFALIGYAFVSALLPVWL
                     ILAPRDYLATFLKIGVIVGLALGIVVLNPELKMPAMTQYIDGTGPLWKGALFPFLFIT
                     IACGAVSGFHALISSGTTPKLLANETDARFIGYGAMLMESFVAIMALVAASIIEPGLY
                     FAMNTPPAGLGITMPNLHEMGGENAPIIMAQLKDVTAHAAATVSSWGFVISPEQILQT
                     AKDIGEPSVLNRAGGAPTLAVGIAHVFHKVLPMADMGFWYHFGILFEALFILTALDAG
                     TRSGRFMLQDLLGNFIPFLKKTDSLVAGIIGTAGCVGLWGYLLYQGVVDPLGGVKSLW
                     PLFGISNQMLAAVALVLGTVVLIKMKRTQYIWVTVVPAVWLLICTTWALGLKLFSTNP
                     QMEGFFYMASQYKEKIANGTDLTAQQIANMNHIVVNNYTNAGLSILFLIVVYSIIFYG
                     FKTWLKVRNSDKRTDKETPYVPIPEGGVKISSHH"
     gene            11367..11570
                     /locus_tag="PN977_001703"
     CDS             11367..11570
                     /locus_tag="PN977_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HDI6041745.1"
                     /translation="MFGNLGQAKKYLGQAAKMLIGIPDYDNYVEHMKTNHPDKPYMSY
                     EEFFRERQNARYGGDGKGGMRCC"
     gene            11581..12537
                     /gene="yjiA"
                     /locus_tag="PN977_001704"
     CDS             11581..12537
                     /gene="yjiA"
                     /locus_tag="PN977_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HDI6041746.1"
                     /translation="MNPIAVTLLTGFLGAGKTTLLRHILNEQHGYKIAVIENEFGEVS
                     VDDQLIGDRATQIKTLTNGCICCSRSNELEDALLDLLDNLDKGNIQFDRLVIECTGMA
                     DPGPIIQTFFSHEILCQRYLLDGVIALVDAVHADEQMNQFTIAQSQVGYADRILLTKT
                     DVAGEAEKLRERLARINARAPVYTVTHGDIDLGLLFNTNGFMLEENVVSTKPRFHFIA
                     DKQNDISSIVVELDYPVDISEVSRVMENLLLESADKLLRYKGMLWIDGEPNRLLFQGV
                     QRLYSADWDRPWGDEKPHSTMVFIGIQLPEEEIRAAFAGLRK"
     gene            12868..14718
                     /locus_tag="PN977_001705"
     CDS             12868..14718
                     /locus_tag="PN977_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000190970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDI6041747.1"
                     /translation="MATIIPTISSCNDKITAGEKRLARILERGLGEECCCWYDIPTGD
                     KHLHPDFMILSPEKGIIFLEVKDWFITKIKKANKSNVLYETQNGIQSLKNPIEQVRRY
                     AFEAVNQLKKDPLLCQQDDRYNGNLLFPYGYGVYFSNITREALNNKFTQEELLGIFPS
                     DLIICKDEINEFMSKEDVSSKIHSLIKYDFKCHATQEQLNRIRWHLYPDVRIEREKRS
                     KNRDEFSVDAPTIISILDTQQEQLARSMRDGHRIIHGVAGSGKTLILYHRCQELAKKN
                     DSEKPILVICYNITLAKKLRSMFINHPFAEKIKVKNFHAWCFQQIKENKITIPEGDNI
                     FENMELALTDGFKTGKIKPEQYSAVLIDEGHDFKPEWLKILAKMTDSQDENLLFLYDD
                     AQSIYQKKKALDFTLSSVDIKATGRTTILNINYRNTQQILHFASCIAFNYLNSHIDSS
                     LTYHKPAAGGMNGDYPNLEHFDTQDEEIARAVEWITEQNEQGIPWSEIAILSPSTHTL
                     SNSLSSVLESKNIPFNLIVSSADKKSWTPEQERISVMPLPSSKGLEFHSVVIIDAART
                     RDNSDDLSEDIKRLYVGFTRARCNLLVTLHGEGALNEHLLKTYEQSPQYM"
     gene            14863..15156
                     /locus_tag="PN977_001706"
     CDS             14863..15156
                     /locus_tag="PN977_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000926091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3387 domain-containing protein"
                     /protein_id="HDI6041748.1"
                     /translation="MLLNSSALATGINKFKLPTTKFQLLQKLLAKEMKSVVDNTSKYT
                     DWSKRDDIKAKLKVELILLLHKHKFPPVANDDVYMGVLAQAENFKEHHMSALN"
     gene            15371..15712
                     /gene="symE"
                     /locus_tag="PN977_001707"
     CDS             15371..15712
                     /gene="symE"
                     /locus_tag="PN977_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endoribonuclease SymE"
                     /protein_id="HDI6041749.1"
                     /translation="MTDTHSIAQPFEAEVSPANNRQLTVSYASRYPDYSRIPAITLKG
                     QWLETAGFATGTAVDVKVMEGCIVLTAQPPTAEESELMQSLRQVCKLSARKQKQVREF
                     IGVIAGKQKVA"
     gene            complement(15759..17852)
                     /locus_tag="PN977_001708"
     CDS             complement(15759..17852)
                     /locus_tag="PN977_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000648235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
                     /protein_id="HDI6041750.1"
                     /translation="MKATEARLLDFLKRSQQFVIPIYQRTYSWTEQQCRQLWDDIIRA
                     GKRDDISAHFIGSVVYIEQGLYQVSGISPLLVIDGQQRLTTAMLLIEALSRHLGEDEV
                     FDGFSAMKLRNYYLLNPYESGEKGFKLLLTETDKDSLLALIKQRPMPENYSHRIMENF
                     TFFDEQIAKLGDDLIPLCRGLAKLLIVDVALNRGQDNPQLIFESMNSTGKALSQADLV
                     RNFILMGLEPEHQTRLYEDHWRPMEVAFGQQGYSEYFDSFMRHYLTVKTGEIPRTDEV
                     YEAFKLHARSQSVAEKGVDHLVEDIHIYAEYYCAMALGKESDKSLATAFQDLRELKVD
                     VAYPFLLALYHDYKNGDLPHEDFLSIIRLIESYVFRRAVCAIPTNSLNKTFATFYKVI
                     IKEKYLESIQVHFLNLPSYRRFPNDDEFKRELKVRDLYNFRSRSYWLRRLENDKRRER
                     VEEFTIEHIMPQNENLSAKWREELGSDWQRVHKELLHTLGNLTLTRYNSRYSDRPFAE
                     KRDIEDGFKHSPLYLNIGLGQCEKWDEAAIRARADRLADLAVQVWQAPALPEEVLAVY
                     RAQPENKTSYSLSDYPFLADGSHSRVLFDHLRDEVMRLDAGITQEVLKLYIAFKAETN
                     FVDVVPQKSRLRLSLNMQFHELVDPKGIAKDVTNVGRWGNGDVEIGFSDLAQLPYVMG
                     LIRQAFEKQMESALV"
     gene            complement(17949..18113)
                     /locus_tag="PN977_001709"
     CDS             complement(17949..18113)
                     /locus_tag="PN977_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000394265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1127 domain-containing protein"
                     /protein_id="HDI6041751.1"
                     /translation="MEFHENKAKAPFIGLVQLWQAVRRWRRQMQARRVLQQMSDERLR
                     DIGLRREDVE"
     gene            18290..19702
                     /locus_tag="PN977_001710"
     CDS             18290..19702
                     /locus_tag="PN977_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HDI6041752.1"
                     /translation="MTRYQHLATLLAERIEQGLYRHGEKLPSVRSLSQEHGVSISTVQ
                     QAYQTLETMKLITPQPRSGYFVAQRKAQPPVPPMTRPVQRPVEITQWDQVLDMLEAHS
                     DSSIVPLSKSTPDVETPSLKPLWRELSRVVQHNLQTVLGYDLLAGQRVLREQIARLML
                     DSGSVVTADDIIITSGCHNSMSLALMAVCKPGDIVAVESPCYYGSMQMLRGMGVKVIE
                     IPTDPETGISVEALELALEQWPIKGIILVPNCNNPLGFIMPDARKRAVLSLAQRHDIV
                     IFEDDVYGELATEYPRPRTIHSWDIDGRVLLCSSFSKSIAPGLRVGWVAPGRYHDKLL
                     HMKYAISSFNVPSTQMAAATFVLEGHYHRHIRRMRQIYQRNLALYTCWIREYFPCEIC
                     ITRPKGGFLLWFELPEQVDMVCVARQLYRMKIQVAAGSIFSASGKYRNCLRINCALPL
                     SETYREALKQIGEAVYRAME"
     gene            complement(19945..20901)
                     /locus_tag="PN977_001711"
     CDS             complement(19945..20901)
                     /locus_tag="PN977_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001678496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HDI6041753.1"
                     /translation="MTNFTTSTPHDALFKSFLTHPDTARDFMEIHLPKDLRELCDLDS
                     LKLEYASFVDEKLRALHSDILWSVKTREGDGYIYVVIEHQSREDIHMAFRLMRYSMAV
                     MQRHIEHDKRRPLPLVIPMLFYHSSRSPYPWSLCWLDEFADPATARKLYTAAFPLVDV
                     TVVPDDEIVQHRRVALLELIQKHIRQRDLMGLIDQLAVLLVTGCANDSQITALLNYIL
                     LTGDEARFNEFISELIRRMPQHRERIMTIAERIHNDGWLLGRERGRKEGKEEGEKSLL
                     RLLLQNGADPEWIQRYTGLSAEQMQALEQPLPESKRDPWIEY"
     gene            21368..22600
                     /gene="mdtM"
                     /locus_tag="PN977_001712"
     CDS             21368..22600
                     /gene="mdtM"
                     /locus_tag="PN977_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtM"
                     /protein_id="HDI6041754.1"
                     /translation="MPRFFARHAATLFFPMALILYDFAAYLSTDLIQPGIINVVRDFN
                     ADVSLAPAAVSLYLAGGMALQWLLGPLSDRIGRRPVLINGALIFTLACAATMFTTSMT
                     QFLIARAIQGTSICFIATVGYVTVQEAFGQTKGIKLMAIITSIVLIAPIIGPLSGAAL
                     MHFMHWKVLFAIIAVMGFISFVGLLLAMPETVKRGAVPFSAKSVLRDFRNVFCNRLFL
                     FGAATISLSYIPMMSWVAVSPVILIDAGGLTTSQFAWTQVPVFGAVIVANAIVARFVK
                     DPTEPRFIWRAVPIQLAGLALLIVGNLLSPHVWLWSVLGTSLYAFGIGLIFPTLFRFT
                     LFSNNLPKGTVSASLNMVILMVMSVSVEIGRWLWFNGGRLPFHLLAVLAGVIVVFTLA
                     GLLNRVRQHQAAELAEER"
     gene            22641..23921
                     /locus_tag="PN977_001713"
     CDS             22641..23921
                     /locus_tag="PN977_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF445 domain-containing protein"
                     /protein_id="HDI6041755.1"
                     /translation="MNKLIELRRAKMLALSLLLIAAATFVVTLFLPPNFWVSGVKAIA
                     EAAMVGALADWFAVVALFRRVPIPIISRHTAIIPRNKDRIGENLGQFVQEKFLDTQSL
                     VALIRRHEPALLIGNWFSQPENARRVGQHLLQIMSGFLELTDDARIQRLLKRAVHRAI
                     DKVDLSGTSALMLESMTKNDRHQVLLDTLIAQLIALLQRDKSRKFIAQQIVRWLESEH
                     PLKAKILPTEWLGEHSAELVSDAVNSLLDDISRDRAHQIRHAFDRATFALIDKLKNDP
                     EMAARADAVKSYLKEDEAFNRYLSELWGDLREWLKVDINSEDSRVKERIARAGQWFGE
                     TLIADDALRASLNGHLEQAAHRVAPEFSAFLTRHISDTVKSWDARDMSRQIELNIGKD
                     LQFIRVNGTLVGGCIGLILYLLSQLPALFPLGNF"
     gene            24036..25187
                     /locus_tag="PN977_001714"
     CDS             24036..25187
                     /locus_tag="PN977_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418755.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacyl-CoA dehydratase subunit D"
                     /protein_id="HDI6041756.1"
                     /translation="MSLVTDLPAIFDQFSEARQKGFLTVMDLKERGIPLVGTYCTFMP
                     QEIPMAAGAVVVSLCSTSDETIEEAEKDLPRNLCPLIKSSYGFGKTDKCPYFYFSDLV
                     VGETTCDGKKKMYEYMAEFKPVHVMQLPNSVKDDASRALWKAEMLRLQKAVEERFGHE
                     ISEDALRDAIALKNRERRALANFYHLGQLNPPALSGSDILKVVYGATFRFDKEALVDE
                     LDAMTARVRQQWEEGQRLDPRPRILITGCPIGGAAEKVVRAIEENGGWVVGYENCTGA
                     KATEQCVAETGDVYDALADKYLAIGCSCVSPNDQRLQMLSQMVEEYQVDGVVDVILQA
                     CHTYAVESLAIKRHVRQQHNIPYIAIETDYSTSDVGQLSTRVAAFIEML"
     gene            25197..25964
                     /locus_tag="PN977_001715"
     CDS             25197..25964
                     /locus_tag="PN977_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418754.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6041757.1"
                     /translation="MAYSIGIDSGSTATKGILLADGVITRRFLVPTPFRPATAITEAW
                     ETLREGLETTPFLTLTGYGRQLVDFADKQVTEISCHGLGARFLAPATRAVIDIGGQDS
                     KVIQLDDDGNLCDFLMNDKCAAGTGRFLEVISRTLGTSVEQLDSITENVRPHAITSMC
                     TVFAESEVISLRSAGVAPEAILAGVINAMARRSANFIARLSCEAPILFTGGVSHCQTF
                     ARMLETHLGMPVNTHPDAQFAGAIGAAVIGQRVRTRR"
     gene            25961..26227
                     /locus_tag="PN977_001716"
     CDS             25961..26227
                     /locus_tag="PN977_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3343 domain-containing protein"
                     /protein_id="HDI6041758.1"
                     /translation="MKEFLFLFHSTVGVIQTRKALQAAGMTFRVSDIPRDLRGGCGLC
                     IWLTCPPGEEIQWVIPGQTEAIYCQQGSDWQCVVHYDSEASTRQ"
     gene            26549..28522
                     /locus_tag="PN977_001717"
     CDS             26549..28522
                     /locus_tag="PN977_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HDI6041759.1"
                     /translation="MDSTFNIYQDILPAFNMYSGLKPCHEKNNQPFDINTEIETIQKQ
                     INYDINHLNDGLIKRVLNLFIHLISNPDNLELTLNRYSSTTEQIIGRTKRNSLHEFEV
                     GDLKIIFNRQDDNESVLTIKHKDISHGCNVKTEQLQQFIKIMEQKAQLPIYIDKNNLK
                     ESIFSVLRNDPQHVDKEQYLPCDKFLTHACKNSNSFEVKLDATHQYQHLNNFMISFDP
                     VENQLTIRDNNNETETISLTNLQWENVLQYYRENHQQPNIAGSRNLTDNIDKIKNTIS
                     TSEIIECASPEIRSSVLNDLYSIANFLPDKNLTPNESWKRFCHTCERFYVAQKSITGD
                     NSERLTRKVSISDAGITMTFKIGDVVINTISTAIPEDESGQRCIEGLNLAEMDLTGID
                     LSKMALRNVNFNGSILRNADFSGTICEGVDFTDCDLRYATFIDASLEKIDFRKVRHLF
                     NINFTNANLRNSNFSGKVLTGVNFTGSDLSNAYLEHIDFTKVIFFPSLIIGAVFDNSN
                     LSEKNLSDKDLTNISCMYTNFTNANLTKCKLLNTNFSAAKFDNTNFTGTKGSNILFNH
                     AWLFNTIFIDTIFKNACFFNAKVNNVSLKKAYIYNDNIDKKANDSTDKQAKNSTEQQD
                     STSFNQARLKKEVNSSFSIPGLTSYQPTYIVDE"
     gene            28777..29619
                     /locus_tag="PN977_001718"
     CDS             28777..29619
                     /locus_tag="PN977_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313321.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiK family protein"
                     /protein_id="HDI6041760.1"
                     /translation="MKKRITFIVFLCAIVAVSLFFVQSCVRKSQHVAGFQNYQATIDG
                     KEITGVTKNISSLTWSAQSNTLFSTINKPATIVEMTTEGDLIRTIPLDFVKDLETIEY
                     IGDNKFVISDERDYAIYVISLNAESEVSILKKIKIPLQETPTNCGFEGLAYSSQDHTF
                     WFFKEKNPIEVYKVTGLLRSDELHISKDKALQRQFTLDDVSGAEFNPQKNTLLVLSHE
                     SRALQEVTVTGDVIGEMSLTKGRYGLSHNIKQAEGIAMDASGNIYIVGEPNLFYRFTS
                     TKSR"
     gene            29687..30865
                     /locus_tag="PN977_001719"
     CDS             29687..30865
                     /locus_tag="PN977_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDI6041761.1"
                     /translation="MNSTSHPVERFSFSTALFGMLVLTLGMGLGRFLYTPMLPVMLAE
                     GEFSFSELSWIASGNYAGYLAGSLLFSFGAFHLPSRLRPFLLASALATGLLILAMAWL
                     PPFLLVFIIRFLAGVASAGMLIFGSTLIMQHTRHPFVLAALFSGVGVGIALGNEYVLA
                     GLHFALSSQTLWQGAGALSAIILLALALLIPSNKHVIPPAPLAKIAQQPMSWWLLAIL
                     YGLAGFGYIIVATYLPLMAKDAGQPVLTAHLWTLVGLSIVPGCFGWLWAAKRWGALPC
                     LTANLLVQAICVLLTLASSSPLLLIISSIGFGGTFMGTTSLVMTIARQLSVPGNLNLL
                     GFVTLIYGIGQILGPALTSMLGNGTSALASATLCGAAALFIAALICGMQIFKLHTNDS
                     "
     gene            31076..36316
                     /locus_tag="PN977_001720"
     CDS             31076..36316
                     /locus_tag="PN977_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001589595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter beta-barrel
                     domain-containing protein"
                     /protein_id="HDI6041762.1"
                     /translation="MASTNAHQIKNNDQNSLCGLGDKIRRLTAGVCLFTQIFFPVMAT
                     AQNVVHAKPQTTVSSPSPLIENNTVPYTLGALESAQSVADRFGISLEELRRLNQFRTF
                     ARGFDNVRQGEELDVPATTLQKSHEQQNAVPPANGENTLENQIASTSQRVGPLLSQDM
                     NSEQASGMARGWASSEASGAMTDWLNNFGTAKISLGVDEDFSLKNSQFDFLHPWYDTP
                     DYLLFSQHTLHRTDDRTQINTGLGWRHFTPSWMSGINLFFDHDLSRYHSRAGLGAEYW
                     RDYLKLSSNAYIGLTGWRSAPELDNDYEARPANGWDLRAEGWLPAWPQLGGKLVYEQY
                     YGDEVALFDKNDRQSNPHAITAGLNYTPFPLLTLSAEQRQGKQGENDTRFAVDLTWQP
                     SSSMQKQLNPDEVAGRRSLAGSRYDLIDRNNNIVLEYRKKELIHLSLQDPVKGKSGEI
                     KPLVSSIQTKYALKGYNIEAAALEAAGGKVRTSGKDITVTLPGYRFTNTPETDNTWSI
                     DVTAEDVKGNLSRHEQSMVVIQAPTLSQKDSLLSVNPLTVAADKKSTTTLTVTAHDSD
                     GTPVPGLALQTRSEGVQDITLSDWTDNGDGSYTQMLTAGTTSGSVTLTPQINGESAVK
                     ESIVVNIVPVVSSRDHSSITIDNVSYYAGDDIKVRVELKDDSNQPVAYQKEELVKAVT
                     VENSKPGATIVWHEEQPGVYAANYPAHKQGTALRAQLSLHNWNAPLQSHIYNIEANQN
                     KARVATLSATNNDVYADKKTFNTLTINVTDESDNPLTNHQVTFKNEKGSAEFVEPPQQ
                     NTDAYGVATINMVSQVAEENTISATLPNGFSQRIIAKFVSDSSTPKFKQLVADPDTII
                     AGNSQGSTLTATVTDFHNNPLKDMKVNFVAPGGSQLDNTTATTDQSGIVRVHLTSSKA
                     GSYSVDASLEADKNIHQSVTITVVPNREQSVMTLNAGSGSAIANNTNTVILTASVKDV
                     YGHPLPDEDVKFTLPASMTGNFTLSSETARTDANGDAVVTLRGTKAGEFTVTATLTRN
                     NTVAHQQVTFIGDTNSAQLQPLTASLNTIVAGDSTGSTLTATILDAYQNPLKDQLVTF
                     QSNDVTLSGTEVTTNTLGQATVTMTSNIAGQHNVVVSRKAQVSDNKTFSLSVLPDESS
                     AKVISITGAEKTITVGENITLRILVQDAFNNVISGQRVRLSAQPTANITIGDTAYTDN
                     NGYAYVNLISTQPGVYQVTATLDNNSSSKVDVNVANGKLELTSSKPETTVHNSEGITL
                     TATARNARDELMPGQIITFSVTPEGATLSNTGEILTDQSGQAKVTLTSNKVNVYTVTA
                     TMGKDVPVQSQVTVAVKADAKTAHVVSVVASPDTITADGVDSSTITSRVEDDYGFPVE
                     GVDVSYALDTKGSPVVNIPTTRTDQSGQVTATITSTLAETLIVNVQVPGTANQSATIT
                     LVAGTADESKSILKSDVDTLKADYQQSAKLTLTLQDKYGNPIVTSDHLEFVQSGPFVN
                     FLKLSDIDYSQRNYGEYTVTVTGGKEGTATLIPMLNGVHQANLSVSLNLIRSIKEMSG
                     HVTANNHTFSTAKFPSEGFAGAYYTLNNDNFEAGKTVDDYMFSSSQSWVSVDASGKVS
                     FANIGDQTSVTISAVPRQGGTTYQTLIKLKGWWVNNGNHTNIWLAANALCHAKNDGYT
                     LPAITHLTSGENQRTQGSLYGEWGNVGAFSSNSQFTQGAYWTSESDDYNRHYYVQMLT
                     GMTGSDADSSPQLTACRKSL"
     gene            complement(36437..37021)
                     /gene="kptA"
                     /locus_tag="PN977_001721"
     CDS             complement(36437..37021)
                     /gene="kptA"
                     /locus_tag="PN977_001721"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2'-phosphotransferase"
                     /protein_id="HDI6041763.1"
                     /translation="MAKYNEKELADTSKFLSFVLRHKPESIGIVLDREGWADIDKLIL
                     CAQKAGKRLTRALLDTVVATSDKKRFSYSSDGRCIRAVQGHSTSQVAISFAEKTPPQF
                     LYHGTASRFLDEIKKQGLIAGERHYVHLSADEATARKVGARHGSPVILTVKAQEMAKR
                     GLPFWQAENGVWLTSTVAVEFLEWPFMPAGLQKQ"
     gene            37271..37954
                     /locus_tag="PN977_001722"
     CDS             37271..37954
                     /locus_tag="PN977_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6041764.1"
                     /translation="MTQQGDAVAGELATEKVGIKGYLAFFLTIIFFSGVFSGTDSWWR
                     VFDFSVLNGSFGQLPGANGATTSFRGAGGAGAKDGFLFALELAPSVILSLGIISITDG
                     LGGLRAAQQLMTPVLKPLLGIPGICSLALIANLQNTDAAAGMTKELAQEGEITERDKV
                     IFAAYQTSGSAIITNYFSSGVAVFAFLGTSVIVPLAVILVFKFVGANILRVWLNFEER
                     RNPTQGAQA"
     gene            37951..>38327
                     /locus_tag="PN977_001723"
     CDS             37951..>38327
                     /locus_tag="PN977_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiG family protein"
                     /protein_id="HDI6041765.1"
                     /translation="MTTQVRKNVMDMFIDGARRGFTIATTNLLPNVVMAFVIIQALKI
                     TGLLDWVGHICEPVMALWGLPGEAATVLLAALMSMGGAVGVAASLATAGALTGHDVTV
                     LLPAMYLMGNPVQNVGRCLGTAEV"
BASE COUNT         9810 a         9587 c         9235 g         9695 t
ORIGIN      
        1 tcccgtgcca ccagatggat tagttcaacg cagtacccga attaaacgca ttcaaaccac
       61 cgccatgttg ttattatttt ttgcggcggt aatcaattat ctcgaccgca gttcgctgtc
      121 ggtagcaaat ttaacgattc gtgaagaatt gggattaagt gccaccgaaa tcggcgcttt
      181 gctctccgtg ttttcactcg cttacgggat tgcgcaactt ccttgcggcc cactattgga
      241 tcgtaaaggc ccgcgcctga tgctgggact ggggatgttc ttctggtcac tgttccaggc
      301 catgtctggc atggtgcaca gctttacgca gttcgtgttg gtgcgtatcg gtatggggat
      361 tggtgaagcg ccgatgaacc catgcggtgt aaaagtcatt aacgactggt tcaacatcaa
      421 agagcgcgga cgcccgatgg gcttcttcaa cgcagcttct accattggcg ttgccgtaag
      481 cccaccgatt ctggcggcga tgatgctggt gatgggctgg cgcgggatgt ttattactat
      541 tggtgtactg gggatttttc tcgccatcgg ctggtatatg ctctatcgca accgcgagca
      601 cgtagaactg actgccgttg aacaagctta tctcaatgca ggtagcgtca atgcccgccg
      661 agatccgctc agttttgccg aatggcgcag cctgttccgt aaccgcacaa tgtggggaat
      721 gatgctcgga ttcagtggca tcaactacac tgcgtggctg tatctggcct ggcttcctgg
      781 ttacctgcaa acagcctata acctggattt aaaaagcaca gggttgatgg cggctatccc
      841 tttcctgttt ggggctgccg ggatgctggt caacggttac gttactgact ggctggtcaa
      901 agggggaatg gctccgatta aaagccgtaa gatctgcatt attgccggga tgttctgttc
      961 tgccgccttt acgctggtcg taccgcaagc gacaacatcc atgacggcgg ttctgctgat
     1021 tggtatggca ctgttttgta ttcactttgc cggaacatcc tgctggggat tgatccacgt
     1081 cgcagtagct tctcgcatga ctgcgtcggt gggcagtatc cagaactttg ccagcttcat
     1141 ctgcgcctct ttcgcgccga tcattactgg ttttattgtt gataccaccc attcattccg
     1201 tctggcacta atcatctgcg gttgcgtcac tgcggcgggt gcactggcgt acatcttcct
     1261 ggttcgtcag ccgatcaacg acccacggaa agattaatct ccctatgccc gataacattt
     1321 tgcttatcgg gcatttttat ggcgattaaa acgtttccca gttctccccg ctatctgcca
     1381 cggccatttt acgcggcgca gctggcgtca cggtttttac cacagccgat gtttcacgct
     1441 gatgttgctg ctgttgctga atccggaaca ctgccacagc ttcggtcagg cgactggcct
     1501 gctcttccag cgcggcggcg gcagcggcag attcttccac cagcgcagcg ttctgttgag
     1561 ttacccggtc catctcagca accgctaatc caacctgatc gataccacgg ctctgctcat
     1621 cagaagcaga agctatttcg cccataatgt ccgtcacgcg ggtcacagcg ctgacaatct
     1681 ctgccatggt ttccccggcg ctttcgacca gcgtagagcc aacatccact ttgcccaccg
     1741 agtcttcaat caggctttta atttcgcgag ccgcctgagc gctacgctgg gccagattac
     1801 gcacttctcc cgcaaccacc gcaaaaccgc gcccttgctc acccgcgcgc gctgcttcaa
     1861 ccgccgcgtt caaagcgaga atattggtct ggaaggcaat gccgtcaatt acgctgataa
     1921 tatcggcgat tttctgcgaa ctggtggaga tatcgcgcat ggtctgcacc acgttatcca
     1981 ccactttgcc gccgcgttgc gccgtttcag aagcacttaa cgccaggtgg ctggcctggc
     2041 gcgcattttc ggcgttctgc ttcaccgttg cggtcagttg ctccatgctg gctgccgtct
     2101 cttccagcga agcggcctgt tgctcggtgc gcgaagagag atcgttattg ccggtagcga
     2161 tttcgctggc accgctatag atggcattgg ccccgttgcg cacatcaccg acggtacgca
     2221 tcagctctcc ctgcatatgg cgcaaactct cagccagttg ccccatctca ttagagccat
     2281 ccacctcaat cggtttcacc agatcgccgc ctgcaatatg acgaatgctg tcaatcaggc
     2341 gattcattgg cgctaccaac gaggctttaa taccgaacca gacggcgaag atgaccgcca
     2401 gtacgacgat catcacgccc accagaatcc acatcgcctg gctgtaggag gcattattat
     2461 cgctgacggc gatatcatag agccgatcgt tttgctccat gtaagccaca tactgcttct
     2521 cgaaaccgtc ctgatatccc tgggttggct gatcaaagaa ctcgttgatt ttgcctgcac
     2581 ctaacagttg gatcagctcc gccagcgcat tgtgataaat atcgtaatta cgtttgatct
     2641 ctgccgctgc ggcggtgctc tgacgcgggt cacgcggcaa cgcttcgtaa tccgcccagt
     2701 ttttttccgc ctgtttcagc gaaatactgg cactctgcat cagctcagca acggttgaac
     2761 cgctaccaat attattctga tccatcatgt agcggatacc cgcgcggttg agggtgttac
     2821 gcgtctgcaa caacgcgacc cagctgccat tcagcgtgga ttgctgctgg cgaatggttt
     2881 gtaaaacagt gaaattttct ttgtcattct ttaaggcatt aaagaacaga ccgcctgatg
     2941 tcagttgtaa aaggccaaaa acggccaaaa ccagcagtaa gctggtcacg atcttgatac
     3001 gttttaacat ggtttctctt tccactagac agatacactg gttgtcggcc tggtggtgga
     3061 aaactttatg ctgtggaaaa gcgtcggcac tgcttttgtt gcagcaaact tgcgtttttt
     3121 gtgagggtca cactctatag cggcagttta tggtacaaag tatcaatctt cttcgaacca
     3181 gtgatactcc tatggcttat agcatcgata tcatctcctg cattaccgaa cggtttatgg
     3241 cgttaacagc cactgaaaag cgtatcgcac aatttattct tgatgacgtc tctgccgcag
     3301 tagagttgcc aattgctgag ctggcacggt taacgcaaac cagtcaggcg tcggtgacgc
     3361 gttttgcccg tgcactgggg tgcaaagatg tccgcgagtt gaaagtcaaa ctggcgcagt
     3421 cactggctgt cgggcagcgg tttattctcg atgtaccgga tcttgacggt gttcagggga
     3481 tctacgaagc gattattagc gtgctggaga cgcatcgtcg ttcgctggat atgaatgcca
     3541 tcagccaggc tgtaagctgg ttaagtcagg cgcggcaaat tcttgcgtta ggaactggcg
     3601 gcggttccac tatctgttca caggagattc agtaccgttt attccgtctg gggctaccgg
     3661 tggtcagtca gaatgatgcc ctgatgatga gaatgatgtg ctcggcagtc atgccgcagg
     3721 atgtggtgat tgtgctttcg ctgggcggtt atacaccgga aatcatcgaa agtgcggcaa
     3781 tcgcccgtca gtatggcgcg cgtgttattg ccattacgcc agcacaaaca ccgctggcgg
     3841 aacaggtgga tctggtattg ccgttactgg ttcaggaaag tgattacatc tttaagccca
     3901 ccccttcccg ctacgcgatg ttagccatgg tcgatgtact ggcaacagaa ctggcaatgg
     3961 caaataaagc gcaggcgaaa gatcgcctgc ggcggataaa actcgtgtta gacagccacc
     4021 gtgggggcgc taaccgccag ccacttggcg attagcattg tcggttatgc ttcttcaggt
     4081 ttggtgatca cgacactctg gttttcacgc tcattggtga taatagccaa agcttcttcc
     4141 cggctgacct gacttgctgg cgttaccaac gtcaccagta cgccagcgac caggctgccc
     4201 agcactgacg gaatacacgg gttaccccag taagccagcc agtcggcatt catcagcacc
     4261 acaattgaca tcaccatacc actcactaac gccgccagcg cgccctgcca gttaaagcgt
     4321 agccagaagc gaccgagaat cgagcagata aacaacccgg acatcagcat cgaaatcatt
     4381 ttggtaatgt agctgatgat gtcgttagag gtcagagcaa agaccagcgc caggccaata
     4441 acaaaggtca acatccagcg tgagtaagtg attgccttat cctccggcgg cattctgccg
     4501 gtaatcatgg tgtagacgtc gcgcatcata atcgccaccg ccgcgatggc atcggagctg
     4561 gcagaagaca tggtggctga aagcccggca atcagcacca ccagcccaag aatggcaggt
     4621 aagaaactgg tcgcgaacaa gaaggcgtag ttgctgtttt ccagatttgg gttcatggtg
     4681 aacgccgcca tgccgataat ggcaggcaaa atcgagaaga acagatataa caccccagta
     4741 ataacaaacg aacggcgtac agagggaaca tctttacccg agtaaatacg ctggcgataa
     4801 gacggtgttg ccagcacacc tacgccaata accagtgcca gcgaaagcgc aggcagcacg
     4861 cccattttat caatcgcaaa caggctcatc gcttttgggt ccatcgcctg ctcaatcgca
     4921 ctccagccgc caacgtgaac aacggcaaga atagccatta atataaagcc aaagaacaga
     4981 attagggcct gaatagtatc tgtccacact accgccgaat aaccgccgat aataacgtaa
     5041 atcgcaaaag ctaaggcaat aattaatttt gccaccgtaa gatcaatacc tgttgcccag
     5101 gcaagataca tacttccacc taatatatgc gcccccagcc agccaataga ggcaataaat
     5161 atcatcaggc cgacaatatt tttaattaaa tggctgccgc cagtataata agatatttct
     5221 tcactcatgg tcataaatcg taatttacgt accggtgcaa acagccacgc caccagtaat
     5281 atacctacgg ccccaccgac gccataaagc attccggccc agccattgct gtagccaaag
     5341 cccactgcgc ccatactgga acccgtgcca accatcgtgg caacggttga ccccagcgtc
     5401 agaaacagcg gcagcgaacg cccgcccagc agaaaatctt ctccgctttt ctgattacga
     5461 gaaacaaacc aaccaagcca aatcatggcg atggcataaa taataaagcc gactaaaaag
     5521 atatgactgt tcataattca catcctgcca gtagagagaa attaatgtaa gagttcccgg
     5581 agtgctgaat gtattcagca ctcaattgcg gtctgttttt ttaattctta aatttattag
     5641 cgggtcgcgt tataacaggt cacatcgact tcgactttac aatcgacaac cagatcagcc
     5701 acacaacaaa tacgtgcagg tggattctcg ccaaaaaatt ctctgaatac tttattaaat
     5761 gactggaaat agcgggcgtc ggtcaggatc actttcacgt gaaccacatc caccaggcta
     5821 taaccggctt cagtcatgat gtcgacacag ttttgaatcg ccagtcgtga ctgttcaacg
     5881 attccgcctt ctaccacttc accgtttttc atgggtgttt gcccggagac gtacagccag
     5941 ccgcccgcct caaccgccct ggcaaaaggc aaatgctgcc cgccagtgcc tgttccacct
     6001 tccgtaccgt aacgtttaat gctcatattc actccttaat gtgaggtctg ccggcgcagg
     6061 aagcgtcccg cacggcctgt aatatgttta tcgggaccat agctcataac gccgttcacc
     6121 atcaccgcct caatgccatc tgccgggcgt ttgggatcag aaaagctggc aacatcacga
     6181 atggtttgtg gatcgaacaa caccaggtcc gcaaaatagc caactttcac cagcccacgc
     6241 tggggcaggc gaaaacggct ggcagaaagc ccggtcattt tgtggatagc cgtggtcagt
     6301 gggaataagc cttcatcacg gcaatagtgg cccagcacgc gagggaaggc gccccataaa
     6361 cgtggatgcg gcatgggatc attgggcagg ccgtcagagc caatcatact gaccggataa
     6421 ctcaacactc ggcggacgtc gcgctcgtcc atgttgtaat agatagcccc ggcaggcatt
     6481 agcagcttcg cggcctccag catactcatc tgccagtcga tggcgatttg cgccagtgtt
     6541 ttcccggcct gctgcggatg tgtttgtgac caggtgatca caatatcgaa ttcgtcggtg
     6601 acttgtttga gatccagcgt tgaagagctg gctgagtaag ggtagacatc gcagccaatt
     6661 tcctggtgtt gacgcatttg gtcaaaaaag gccaacgttt cttttgtccg cccccagttt
     6721 ttcgccccgg cgcatttatg gtgggaaacc acgacaggca ctttgccatg acgcccaata
     6781 cgaaacgcct catccagggc atcgagaatc ggttcaaatt cagaacgcag atgggtggta
     6841 taaacgccct tctcaccggc taattcttcc gccagcgcca tgacttcttc ggtagtcgcc
     6901 tgaaatgccg ttgcataggc cagcccggaa cttaacccca gcgccccctg actcaacgcc
     6961 agacgtaagt cggcacgcat agcggcaatt tcatccaccg tcgccgggcg aaacaggtcg
     7021 tccatatggt tattacgcag cgccgtatga ccaatcagcg tgccaacgtt cagtgacggc
     7081 ctggctgctt ccactgcctg ggcatagctt tcaacggtag gataaataaa gtgctctgct
     7141 tcccccaaca gattcatcgg gtcaggaact tcacctttca ttttcgccga cgccgcacta
     7201 atcccgcagt taccgacaat caccgtagtg atcccctggc tgatctttgg cagatattcc
     7261 gggatgcgaa taacgttgat atcgtcatgg gtatgaacgt cgataaaccc tggtgccagc
     7321 acccgccctt ccccgtcaat gacctgctgc gccgtaacgt taagcgcagg ggcaatatcg
     7381 acaatccgat cacctgttat tgccacgtca ccgcgaaatt cagggccgcc gctaccgtcg
     7441 ataacggtca catttttgat taaccagtca acctgcatgt tcgtcactcc atttctgcaa
     7501 tggtgacaag ttaaccattt acaaggacga aaaacagtgg aaaactacgc aaaacccaga
     7561 tgattttatg atacttttat tcataaataa taagataaat aaactaaata tttatatttc
     7621 atttagttac aaatgatact cacatccgat acggtgaaaa acttaaaagg taaatgttat
     7681 gaaataccac tcagaacctc tggttcccca taaatctgcc ctgatgcaaa tgcccgcaaa
     7741 tctccttgcc gaagatgtct gtttacctgc cgcgatcatt aaaaagcagg ccctggagaa
     7801 caacattacg tggatgcaac gctacgctga cgcgcgcggc gtttcactgg caccgcacgg
     7861 taaaaccacc atgacgccgt ggatttttca ggcgcagcag gcggctggtg cctgggcaat
     7921 aggtgtcggt agcgcatggc aggctggtgc ggcaatggcg agcggtattc agcgagtgct
     7981 gatggttaac cagctggtcg gcaaggcgaa tatgcagttg attgcccagt tgcaacgaca
     8041 ttacccgacg gtcgatttta tcagctgtat cgacagcatt gagaacgccc gcgctttgtc
     8101 tgcctttttt gccagtcagc aacagacact gaacgtgatg attgagttgg gtgtccccgg
     8161 cgggcgctgt ggttgccgct caagggatgc cgcactgaca ctggctaaac aggcagccca
     8221 attaccgggg ttacgcctgc gggggcttga gttatatgaa ggcgttttac acggtgacga
     8281 tccacaaccg caggtagaag ctctgctgcg ggatgccgca caactggcct gtgatatggc
     8341 aagtctggtt gatggcgagt ttattctcac cggagcaggc acggtctggt atgacgtggt
     8401 gtgcaatatc tggctggcgg cagaaaagcc tgataactgc cgcattgtta ttcgccctgg
     8461 ctgctacatc actcacgaca ccgggatcta cgacgcggca caacaacagt tgcttgcccg
     8521 tgatcctgtg gcttgcgatc tggcaggcga tctcacatct gcactggaac tggtcgccat
     8581 ggtgcaatcg gtgccggaag ctgatcgtgc ggtggttaat tttggtaagc gtgattgcgc
     8641 cttcgacgcc ggactgccgc aaccagtcgc ccactatcgc aacggcaaat cactggcatt
     8701 tgatccacag gcgattcgca gtacaggcat tatggatcag cactgcatgt tgcagttggg
     8761 tgccgacagc gacgtgcaag tgggggatat tctggtgttt ggcacatcgc atccgtgcct
     8821 gaccttcgac aaatggaaaa cgttgttatt gactgatgac gactacaacg tactggcaga
     8881 attagacact ctcttctaag aacgcccctt cggttaatta tcgaaggggc atttttattg
     8941 tgatctcaat cacatatcca cccgacgaat aattcttgcc caaccgctca ccgccattcc
     9001 tgaccgctta catccctaaa ataaccactc agttatttac cttactttac gcgcgcgtaa
     9061 ctctggcaac atcactgcag gatagcggtc aatttacctc ctcaaacgca acgcaaacct
     9121 agaacggctt cggccaacta ttaatcaata catgccaggt tttactatgg atactaaaaa
     9181 gatattcaag cacataccct gggtgattct cggaatcatc ggtgcattct gcctcgcggt
     9241 agttgcatta cgtcgggggg agcacgtcag tgccctgtgg atcgtggtcg cctctgtgtc
     9301 ggtgtatctg gtggcttatc gctactacag tctgtacatc gcccagaagg tgatgaaact
     9361 cgaccccacg cgcgcgacgc ctgcggttat taacaacgac ggtctgaact atgttccgac
     9421 caaccgttac gtgttgtttg gtcaccactt cgccgctatc gccggtgctg gtccgctggt
     9481 tggtccggtt ctcgccgcgc agatgggcta cctgcctggc acgctgtggc tgctggcggg
     9541 ggtagtgctg gccggtgcgg tacaggactt tatggtgctg tttatctcct ctcgccgtaa
     9601 cggcgcatct ctcggtgaga tgatcaaaga agagatggga ccagtaccgg ggactatcgc
     9661 gctgtttggc tgtttcttaa tcatgatcat catccttgcc gtcctggcgc tgattgtggt
     9721 taaagccctg gccgaaagtc cgtggggtgt cttcaccgtt tgctcaaccg taccgattgc
     9781 gctgtttatg ggtatctaca tgcgctttat tcgtccgggg cgtgtgggtg aagtctctgt
     9841 cattggtatc gtgctgctgg ttgcctctat ctacttcggt ggcgtgattg ctcacgatcc
     9901 gtactggggt ccggcactga cctttaaaga caccaccatt accttcgcgc tgattggcta
     9961 tgcgtttgtt tccgcactgc tgccagtgtg gctgatcctc gcaccgcgcg actatctggc
    10021 aaccttcctg aaaatcggcg ttatcgtcgg cctggcgctg ggtatcgtgg tgctgaaccc
    10081 ggaactgaaa atgcctgcga tgacccagta cattgacggt actggcccgc tgtggaaagg
    10141 cgctctgttc ccgttcctgt tcatcaccat cgcctgtggt gcggtatctg gcttccacgc
    10201 gctgatctct tccggtacga cgccgaaact gctggctaac gaaaccgacg cgcgtttcat
    10261 tggctacggc gcaatgctga tggagtcctt cgtggcgatt atggcgctgg ttgctgcgtc
    10321 catcatcgaa ccgggtcttt acttcgcgat gaacaccccg cctgctggcc ttggcatcac
    10381 catgcctaac ctgcatgaaa tgggtggcga gaacgcgccg atcatcatgg cgcagctgaa
    10441 agacgttacc gcacacgcgg cagcgaccgt cagctcctgg ggcttcgtga tttcgccaga
    10501 gcagatcctg caaacagcga aagatattgg tgagccttct gtcctgaacc gtgcaggtgg
    10561 tgcgccgacg ctggcggtag gtatcgctca cgtgttccac aaagtgctgc cgatggctga
    10621 catgggcttc tggtatcact tcggtattct gttcgaagcc ctgttcatcc tgaccgcgct
    10681 ggatgcgggt acccgttctg gccgctttat gctgcaagac ctgctgggta acttcatccc
    10741 gttcctgaaa aaaaccgact ctctggttgc cggtatcatc ggtactgcgg gctgtgtggg
    10801 tctgtggggg tacctgctgt atcagggcgt ggtcgatccg ctgggcggcg ttaagagcct
    10861 gtggccgctg ttcggtatct ccaaccagat gctggcagcc gtagcgctgg tactgggcac
    10921 cgttgtgctg attaagatga agcgcaccca atacatctgg gtaactgttg ttccggctgt
    10981 atggctgctt atctgcacca cctgggcgct gggcctgaaa ctgttcagca ccaacccgca
    11041 gatggaaggc ttcttctaca tggcaagcca gtacaaagag aagattgcta acggtactga
    11101 cctgacggca cagcagattg ccaacatgaa ccacatcgtt gtgaacaact acaccaacgc
    11161 gggcctgagt attctgttcc tgattgtggt gtacagcatc atcttctacg gtttcaaaac
    11221 ctggctgaag gtgcgtaaca gcgacaaacg tactgacaaa gaaactccgt acgttccaat
    11281 cccggaaggc ggcgtgaaga tctcttcgca ccactaaccg tgtttagccc cgcttcggcg
    11341 gggctttgtt cttatcagag tgaactatgt ttggtaactt aggacaggca aaaaaatatc
    11401 tcggccaggc ggcgaagatg ttgattggca ttccagacta cgacaactac gtcgagcata
    11461 tgaagaccaa ccatcccgac aagccgtaca tgagctatga agaattcttc cgcgaacgcc
    11521 agaacgcgcg ctacggcggc gatggtaaag gcggtatgcg ctgttgttaa tggagagagc
    11581 atgaacccga ttgcagttac cctactcacc ggttttttag gcgcgggcaa aaccaccctg
    11641 ctgcgccata ttcttaatga acagcacggc tacaaaattg ccgtgattga aaacgaattc
    11701 ggcgaagtct ccgttgatga tcaactgatt ggcgatcgcg ccacgcagat caaaacgctg
    11761 accaacggct gcatctgttg ttcgcgctcc aacgagctgg aggacgcgct actcgacctg
    11821 ctggataatc tcgacaaggg caatattcag ttcgaccgtc tggtcattga atgcacaggc
    11881 atggccgatc ccggcccgat tattcagacc tttttctccc atgagatttt atgccagcgt
    11941 tacctgctgg acggcgtgat tgcgctggtg gatgcggtac atgccgatga gcagatgaac
    12001 cagttcacca tcgcccagtc acaggttggc tacgccgacc gtattctgct gactaaaacc
    12061 gacgtcgcag gtgaagcaga aaaactgcgc gaacgcctgg cacgcatcaa cgcccgcgca
    12121 ccggtctaca ccgtcaccca cggcgacatc gacctgggcc tgttgttcaa caccaacggt
    12181 tttatgctgg aagaaaacgt cgtcagcacc aaaccgcgtt tccactttat cgcggataaa
    12241 caaaacgata tttcgtcgat tgtggtggaa ctggattacc cggtagatat cagcgaagtt
    12301 tcccgcgtga tggaaaacct gctgctggaa tcggcggata aactgctgcg ttacaaaggg
    12361 atgctgtgga ttgacggcga acctaaccgc ctgctgttcc agggtgtcca gcgcctctac
    12421 agcgccgact gggacaggcc atggggcgat gaaaaaccgc atagcacgat ggtgtttatc
    12481 gggattcagc tgccggagga agagattcgg gcggcgtttg cggggttaag gaagtaatac
    12541 ccttaaccta ctaatacgaa gcggacgagg ataaaaccct atcattttcc gttcgcttca
    12601 ctattttatt tcgttccaac acccattaaa attaaaaata taattctgcc ataatttcct
    12661 gaacacagat gtaattacat tgccaccact catactttta aaaataattt atatgataga
    12721 cgtcctattg aggcgtaata gatgtattaa tttccaaata cgcaaacatt ctggtacagt
    12781 ccaaaaaaga aaaatcatca accaaaacaa tcagcaaata aattaacaac gattgaatca
    12841 taatcaacac gaatacaaga gattaacatg gcaacaataa tcccaacaat cagtagctgc
    12901 aacgataaaa ttactgccgg agagaaaagg ctggcaagaa ttctggaaag aggactaggc
    12961 gaagagtgct gctgttggta tgacattccc acgggtgata agcatcttca cccagatttt
    13021 atgatacttt cgccagaaaa aggcattatt tttctcgagg taaaagattg gtttattaca
    13081 aaaataaaaa aagccaacaa gtctaatgtg ttgtatgaga cacaaaatgg tattcaatct
    13141 ttaaagaatc cgatagaaca agttaggcga tatgctttcg aagctgtaaa ccagttaaaa
    13201 aaagaccccc tattgtgcca acaggatgat cgttacaatg gcaatcttct tttcccctat
    13261 ggatatggcg tctactttag taatattaca cgagaagcat taaataataa atttactcaa
    13321 gaagagctgc tcgggatctt cccatcagat ctcatcatct gcaaagacga aataaatgaa
    13381 ttcatgtcaa aagaggatgt ttcatccaaa atacattcgt tgattaaata tgacttcaaa
    13441 tgtcatgcta cacaagaaca gttgaacagg attagatggc acttataccc cgatgtcaga
    13501 atagaaaggg aaaagagaag taaaaaccgt gatgaattta gtgtcgatgc accaaccatc
    13561 atttctatcc tggatactca acaggaacaa cttgccagaa gtatgagaga tggccaccgg
    13621 atcattcatg gtgttgcagg ttcagggaag acattgatcc tctatcatcg ctgtcaggaa
    13681 ttagcgaaaa aaaatgacag cgaaaagcca attcttgtca tttgctacaa tattacttta
    13741 gccaaaaaac ttcggtcgat gttcatcaat catccctttg cagagaaaat aaaggttaaa
    13801 aattttcatg cttggtgttt ccaacaaatt aaagaaaaca agatcactat tccagaaggg
    13861 gataatattt ttgaaaatat ggagctagcg ttgactgatg gtttcaagac aggaaaaata
    13921 aaaccagaac aatacagcgc tgtattaatt gatgaaggac atgattttaa gccagaatgg
    13981 ttaaaaatcc tggcaaaaat gactgactct caggacgaga atttgctatt cctttatgat
    14041 gatgctcagt caatttacca aaaaaagaaa gcattagact ttacactgtc cagcgtagat
    14101 attaaagcga caggacgcac aaccattctt aatataaatt atagaaacac acagcaaatc
    14161 ctacactttg ccagttgcat tgcatttaat tatcttaata gccatatcga tagttcttta
    14221 acctatcata aacctgctgc gggaggaatg aatggcgatt atcccaacct tgaacatttc
    14281 gacacacagg acgaagaaat agcacgagca gtcgaatgga ttacagagca aaatgaacaa
    14341 gggattccgt ggtctgaaat tgctatacta agtccatcaa ctcacaccct ttctaactct
    14401 ctaagctctg tacttgaatc gaaaaacatc ccctttaatc ttattgtaag ctctgcagat
    14461 aaaaagtcct ggacgccaga acaagagcgt atttctgtta tgccattacc cagcagtaaa
    14521 ggacttgagt ttcactcagt tgtcattatt gatgcagcga gaacacggga taatagcgat
    14581 gatttgagtg aggatataaa gagactctat gtaggtttta cccgagcacg ttgcaatctc
    14641 ttggtaacct tgcacggtga aggtgcgtta aatgagcatc tactaaaaac ctatgagcaa
    14701 agcccacaat atatgtaaaa atgataaatt atctccataa cgcccggtaa cgctgtaccg
    14761 ggcgttatga atgtatatct aagatattag tcacgcctga tagccgctac caaaaacgcc
    14821 atcatctccc tctcatcttt gctgtgataa aagcgtacca ttttgctgtt aaattccagc
    14881 gctttagcca ctggaattaa caagttcaaa cttccgacaa cgaagttcca gttattacaa
    14941 aaattactgg caaaagagat gaaaagcgta gttgacaaca catcgaaata tactgactgg
    15001 agtaaacgcg atgatattaa agcgaaactg aaagttgaac ttattctgct tctacacaag
    15061 cataagttcc cgccagtagc gaatgatgat gtttatatgg gtgtgctggc acaggcagag
    15121 aattttaaag aacatcatat gagtgcttta aattaattat gaaggccgga tacactttgt
    15181 tatccggcca ttgtgaatta acgttaattt ctcttccaga tatttccagt aacgctttta
    15241 ttattttcta tttttagctg gactttccct gtatttactg atgatatgtc caggtattta
    15301 gcgcggtgcg aatgtgcgcc aacacacccg caccgctcat cacaatcact atttatggag
    15361 aatagtaatc atgactgaca cgcattctat tgcacaaccg ttcgaagcag aagtctcccc
    15421 ggcaaataac cgtcaattaa ccgtgagtta tgcgagtcgc tacccggatt acagccgtat
    15481 tcccgccatc accctgaaag gtcagtggct ggaaaccgcc ggttttgcta ccggcacggc
    15541 ggtagatgtc aaagtgatgg aaggctgtat tgtcctcacc gcccaaccac ccaccgccga
    15601 agagagcgaa ctgatgcagt cactgcgcca ggtgtgcaag ctgtcagcgc gtaaacaaaa
    15661 gcaggtgcgg gaatttattg gggtgattgc cggtaaacag aaagtcgcct gatacattga
    15721 cccggtagcg ccagtgctac cgggtattat tcgccgtgtt agaccaacgc gctctccatc
    15781 tgtttttcaa atgcctgacg aattaatccc ataacgtaag gaagttgtgc gaggtcgctg
    15841 aaaccaattt ccacatcacc attgccccag cgcccaacat ttgtcacatc tttggcaata
    15901 cctttcggat cgaccagttc atgaaactgc atattaagcg acaatcgcag tcggcttttt
    15961 tgcggcacaa catcaacaaa attcgtttcc gctttaaacg caatatagag cttcaatact
    16021 tcctgcgtga tccctgcgtc caggcgcata acttcatcgc gaagatgatc aaataacacc
    16081 cggctatgcg aaccatcagc aagaaaagga taatcactca ggctataact ggttttgttt
    16141 tcaggctgcg cccgataaac agctaaaacc tcttcaggaa gagcaggcgc ttgccagacc
    16201 tgaaccgcga gatcggccag acgatcggca cgggcgcgaa tggcggcttc atcccatttt
    16261 tcgcactgtc caagaccgat attcaaataa agcgggctat gcttaaagcc gtcttcaata
    16321 tcgcgttttt ccgcgaaagg tctgtcactg tagcgggagt tatagcgcgt taatgtgaga
    16381 ttccccaacg tatgtaacag ttctttatgc acacgctgcc agtcgcttcc cagctcttca
    16441 cgccatttag ccgacaaatt ttcgttctgc ggcataatat gctcaatcgt aaactcttcc
    16501 acgcgctctc tgcgtttatc gttttccagt cgtcgtaacc agtagctgcg actgcggaag
    16561 ttatagagat cgcgaacttt taattcccgt ttaaattcat catcgttggg gaaacgacga
    16621 tatgaaggca gattcagaaa atgtacctga atactttcca gatatttttc tttaataatg
    16681 accttataaa aggtagcaaa cgtcttgtta agagaatttg tcggaatcgc acatactgca
    16741 cggcggaaaa cataagattc aattaaacga attatgctca ggaaatcttc gtgaggcaaa
    16801 tcgccatttt tatagtcatg ataaagcgcc agtaagaaag gatacgccac atcgaccttt
    16861 aactcgcgca aatcctgaaa agccgtagca agcgatttgt cactttcttt acccaatgcc
    16921 attgcacagt aatactccgc gtagatgtga atatcttcaa ccaggtgatc cacgcctttt
    16981 tcagcaacac tctggctgcg ggcatggagt ttaaatgcct catagacttc atctgtacga
    17041 gggatctccc ccgtttttac cgtcagataa tgacgcataa agctgtcaaa atattcgctg
    17101 taaccttgct gaccaaaagc gacttccatt ggacgccagt gatcttcata caaccgggtt
    17161 tgatgctctg gttcgagacc catcagaata aaattgcgca ccagatcggc ctgacttaac
    17221 gccttaccgg tagagttcat actttcaaaa atcagttgcg gattatcctg accacgatta
    17281 agcgccacat cgacaattaa taattttgct aacccacgac ataaggggat caagtcatca
    17341 ccgagtttgg caatttgctc atcaaagaaa gtaaagtttt ccattattcg atgggaatag
    17401 ttttctggca ttggtctttg ttttattaac gccagcaaac tgtctttatc agtctcggtc
    17461 agcagtaatt taaatccttt ctcgccggac tcataaggat tgagtaaata ataattacgc
    17521 aatttcattg ctgaaaagcc atcaaaaact tcgtcttcgc caagatgacg cgataaagcc
    17581 tcaatcagca acattgcggt cgtcagcctt tgttgaccat caatgaccag taacggagaa
    17641 ataccagaaa cctgatacaa cccctgctca atataaacaa ccgaaccgat aaaatgcgct
    17701 gatatatcgt cacgctttcc ggcacgaatg atgtcgtccc aaagttgccg acattgttgt
    17761 tctgtccatg aataagtccg ttgataaatg ggaatcacaa actgctgcga acgttttaaa
    17821 aaatcgagta ggcgggcttc tgtagctttc attctgacgt ctttccgtga agcgatgatt
    17881 tcgcaataat aaccacttcc ttgtggaggt tatagagaaa tcctgtttat ccgcgacgaa
    17941 ctgccccatc actccacatc ctccctgcgt aacccaatat ccctcaaccg ctcatcactc
    18001 atctgctgta acacccgccg ggcctgcatt tgccgccgcc aacgcctcac tgcctgccag
    18061 agttgcacaa gtccaataaa cggtgcttta gctttgtttt cgtgaaattc catctgctgc
    18121 ctcctcatct ttcagaggct tcatcatccg ccttctggca gttcacaata cagatgcaaa
    18181 tattagtttt atttaacata cagacaggtt aaaacagcat ctgcatccgg ttttttagcc
    18241 tcatctgtac cggtttttct ctctgtatgg tccaaataag gaatacagca tgacgcgtta
    18301 tcaacatctg gcgactttgc ttgccgagcg gattgagcaa gggctgtatc gtcacgggga
    18361 gaaattgccg tcggtgcgca gcttaagtca ggagcacggc gtcagcatca gtaccgtgca
    18421 gcaggcgtac cagacgctgg aaacgatgaa gctcatcact ccgcagccgc gttcgggtta
    18481 ttttgtcgca caacgtaaag cccagccgcc agtaccgccg atgacgcgtc cggtgcagcg
    18541 cccggtggaa attacccagt gggatcaggt gctggatatg ctggaggcgc atagcgacag
    18601 ttccattgtt ccgttaagta aaagcacgcc ggatgtcgaa acgcccagcc tgaaaccgct
    18661 gtggcgggag ctaagccggg tggtgcagca taatctgcaa accgttctcg gttatgactt
    18721 gttagccggc cagcgggtat tgcgcgagca gattgcccgc ctgatgctcg acagcggctc
    18781 ggtggtcacc gccgatgaca tcatcatcac cagcggctgc cataactcga tgtcgctggc
    18841 gttgatggca gtgtgtaaac cgggcgatat tgtcgcggtc gaatccccct gttattacgg
    18901 ttcgatgcag atgctgcgcg gcatgggcgt gaaagtgatt gaaatcccca ccgatccaga
    18961 aacaggcatc agcgttgaag cgctggaact ggcgctggag cagtggccga ttaaaggcat
    19021 cattctggtg ccaaactgta ataatccgct gggatttatt atgccggacg cgcgcaaacg
    19081 ggccgttctc tctctcgctc agcgtcatga tattgtgatt tttgaagatg atgtctacgg
    19141 cgaactggcg acggagtatc cgcgcccgcg gaccattcat tcctgggata tcgacgggcg
    19201 agtgctgttg tgcagctcgt tcagtaaaag tattgctcca ggcctgcgcg tgggttgggt
    19261 cgcaccgggg cgctatcacg ataaactgct gcatatgaaa tatgccatca gcagctttaa
    19321 tgtgccgtcc acgcaaatgg cggcggcaac gtttgtgctg gaaggtcact atcatcgcca
    19381 tatccggcgg atgcggcaga tctatcagcg caatttggcg ctttatacct gctggatacg
    19441 ggaatatttt ccctgcgaaa tctgtattac gcgcccgaaa ggcggatttt tactgtggtt
    19501 cgaattgcct gaacaggtcg atatggtttg cgttgcgcgg cagctatacc gcatgaaaat
    19561 ccaggtggcg gcaggctcga ttttctcagc ttccggcaaa taccgtaatt gtctgcgcat
    19621 caactgcgct ttgccgctca gcgaaaccta tcgcgaagca ctgaagcaaa ttggcgaagc
    19681 cgtgtatcgg gcaatggaat gaattcgaaa tagtggcgag ccccctgatt tcctctcacg
    19741 aatagcgtgt gactcgtttt atctttgcag ggtcatctcc acgttccagc atggcttagg
    19801 caaaatgcgc ttcgttcatg ccggatgcca tgtgaacacc ttaccaggtc tacaaaatct
    19861 ttaaaattca atatatttaa tgggttatgt agacctgata agcgtagcgc atcaggctgc
    19921 tttgcgtttg tcatcagtct ctgattagta ctcgatccat ggatcgcgct tgctttcagg
    19981 caagggctgc tctaatgcct gcatttgctc tgccgaaagt ccggtatatc gttgtatcca
    20041 ttcaggatcc gccccattct gcaacaacaa tcggaggagg ctcttttccc cttcttcttt
    20101 cccttctttc ctccccctct cccttcccaa cagccatcca tcattatgaa ttcgctctgc
    20161 aatcgtcatt attcgctccc tgtgttgtgg cattcgacgg ataagttcgc tgataaactc
    20221 attaaaacgc gcttcatcgc cagtcagtaa aatgtaattt aacagcgcgg ttatctggct
    20281 gtcattagcg caccctgtaa ccagtaatac tgccagttga tcaataagcc ccattagatc
    20341 gcgctggcga atatgctttt ggatcaactc cagcagggcg actctgcgat gctgcacaat
    20401 ctcgtcgtct ggcacgacag tgacatccac cagcgggaac gctgcggtat aaagcttccg
    20461 cgcggtagcc gggtcggcaa attcatccag ccagcacaaa gaccaggggt aaggactacg
    20521 gctactgtga taaaacagca tcgggatgac cagcggtagc ggccggcgtt tatcatgctc
    20581 gatatggcgc tgcatcaccg ccatggaata tcgcatcagg cgaaaggcca tatggatatc
    20641 ctcgcggctc tgatgttcaa tcaccacata aatatagcca tctccttcgc gggtctttac
    20701 cgaccacaga atatcggaat gtagcgcccg caatttttca tcgacaaagc tggcgtattc
    20761 cagttttaag ctgtcgagat cgcacagttc acgtaaatct ttgggtaagt ggatctccat
    20821 aaaatcccgc gcggtgtcag ggtgggtgag aaaagattta aataatgcgt catgcggcgt
    20881 gctggtcgtg aagtttgtca tggcgatccg tcacctgtag ataagatggc ccgactgtag
    20941 cgactaacaa aaccattctc acccggcaat ttctcgttcc ccgaggtgct ttccaggcta
    21001 tttttgttcc tctgcaaacg atacattttt ccggaaacag gctcgcgttt tacatttcct
    21061 gtacccggca gatattcccc tttccaccgc actcttttga ttaaccgcaa attttaatta
    21121 cacttaaggt gtatattttc tatgcaaccc atcaattcaa gaggtgtaat gtgctgatga
    21181 ctatttgtaa tcgttataca ttctgacctg gagtcagaag gtatttctct gtctgtgtgt
    21241 tcacaggcag tgtggttgat tacatgaaat tcagtacatt tgcccttctc gcctgccttt
    21301 cgtcattacc gacggtattg aatttcgttt tccccattgg ggttctccgg acaaggagtt
    21361 gtttgttatg ccacgttttt ttgcccgcca tgccgccacg ctgtttttcc cgatggcgtt
    21421 gattttgtat gactttgctg cgtatctgtc gacggacctg atccagccag ggatcattaa
    21481 tgtggtccgt gattttaatg ccgatgtcag tctggccccg gctgccgtca gtctctatct
    21541 cgctggaggc atggcattgc agtggctgct ggggccgctt tccgacagga ttggccgcag
    21601 gccggtgctg attaacgggg cgttaatttt tacccttgcc tgcgccgcga caatgttcac
    21661 cacgtcaatg acacaatttc ttatcgcgcg tgcaattcag ggcaccagta tctgttttat
    21721 tgccaccgtt ggttatgtca cggtgcagga ggcgttcgga cagacaaaag ggatcaagtt
    21781 gatggcgatt atcacctcca tcgtactgat tgcgccgatt atcggcccgc tttccggcgc
    21841 agctctgatg cactttatgc actggaaagt cctttttgcc atcattgcgg ttatgggttt
    21901 tatctcattt gttggcttac tgttggcgat gccagagacg gtgaagcgcg gcgcggttcc
    21961 gtttagcgcc aaaagcgtct tgcgcgattt tcgtaatgtc ttttgcaatc ggctgttcct
    22021 ctttggcgca gcaaccatct ctttaagcta tatcccgatg atgagttggg tggctgtctc
    22081 gccggtgatc ctgattgacg caggcggctt aacgacctcg cagttcgcct ggacacaggt
    22141 tccggtgttc ggcgcggtga ttgttgcgaa tgccatcgtg gcgcgtttcg ttaaagatcc
    22201 gaccgaaccg cggtttatct ggcgtgccgt gcccattcaa ctggccggcc tcgcactgtt
    22261 gattgtcggc aatttgctgt cgccacacgt ctggctgtgg tcggtgctgg gcaccagtct
    22321 gtatgctttc gggattggtt tgattttccc gaccttattc cgctttacgc tgttttccaa
    22381 taacttaccg aaagggaccg tctccgcatc gctaaatatg gtgatcctga tggtgatgtc
    22441 ggtctcggtc gaaatcggcc gctggctgtg gtttaacggc ggtcgcttgc cgtttcatct
    22501 gttagccgtt ttagcgggcg ttatcgtcgt tttcaccctg gcgggattgc tcaatcgcgt
    22561 gcgccagcat caggctgccg agctggcgga ggagcggtga tttttgcgcg atcctgccgt
    22621 caggctctat tcttaacgtt atgaataaac tcattgaact cagacgcgcc aaaatgttgg
    22681 cgctctcttt actgcttatc gccgctgcta cctttgtcgt tacgctgttt ttgccgccca
    22741 atttttgggt gagcggcgtg aaggcgattg ctgaagcggc gatggtcggc gcgctggcgg
    22801 actggtttgc ggtggtggcg ctatttcgcc gcgtgccgat tccgatcatt tctcgtcata
    22861 cggcgattat cccgcgtaat aaagaccgga ttggcgaaaa tctcggtcag ttcgtgcagg
    22921 aaaaatttct cgatacccaa tcgctggtgg cattgattcg acgtcacgaa ccggcgttgt
    22981 tgattggcaa ctggtttagt cagccagaaa acgcccgccg cgttggtcag catctgttgc
    23041 agatcatgag tggttttctt gaactgaccg atgatgcgcg tattcagcgc ctgcttaagc
    23101 gcgcagtcca tcgggcgatt gataaggtcg atctttccgg caccagtgcg ttgatgctgg
    23161 agagtatgac caaaaacgat cgtcatcagg tgctgctgga tacgctgatc gcacagttga
    23221 tcgcccttct ccagcgcgat aaatcgcgca agtttatcgc ccagcagatt gttcgctggc
    23281 tggagagtga gcatccactg aaagccaaaa ttttgcccac tgaatggctg ggcgaacata
    23341 gcgcggagtt ggtttctgac gcggtgaatt ctttgcttga tgatattagt cgcgatcgtg
    23401 cgcatcagat ccgccatgcg tttgatcgcg ccaccttcgc cctgatcgac aagctgaaaa
    23461 acgatccgga aatggcagcg cgagccgatg ccgtaaaaag ctatctgaaa gaagatgaag
    23521 cttttaatcg ctatctcagt gaattgtggg gggatttacg ggaatggctg aaagtggata
    23581 tcaacagtga agattctcgt gtgaaagaac gtatcgcacg agcgggtcaa tggtttggtg
    23641 aaacgttaat tgccgatgat gccttgcggg cgtcgttaaa tggtcacctg gaacaagccg
    23701 cgcaccgcgt cgcgcctgag ttttccgcat tcctgacgcg ccacatcagc gatacagtaa
    23761 aaagctggga tgcgcgggat atgtcgcggc aaatcgagtt aaatattggc aaagatctgc
    23821 agtttatccg tgtcaacggt acgctagttg gcggttgtat tgggctaatt ttgtatttgc
    23881 tgtcgcagct cccggccttg ttccccctcg gcaattttta gaaatccatg aaaaactatt
    23941 ggctaaccgc gagagagatc aaataaacca cactaaaaga atggtaatga tgtggcctct
    24001 tttcattatc tcccgtggta cggggaagga aaatcatgtc acttgtcacc gatctacccg
    24061 ctattttcga tcagttctct gaagctcgcc agaaaggctt tctcaccgtc atggatctca
    24121 aggagcgcgg cattccgctg gttggcacct actgcacctt tatgccgcaa gagatcccga
    24181 tggcagccgg tgcagttgtg gtttcgctct gttccacctc cgatgaaacc attgaagaag
    24241 ccgagaaaga tctgccgcgc aatctctgcc cgctgattaa aagcagctac ggcttcggca
    24301 aaaccgataa atgcccctac ttctactttt ccgatctggt ggtgggtgaa accacctgcg
    24361 acggcaaaaa gaaaatgtat gaatacatgg cagagtttaa gcccgtgcat gtgatgcaat
    24421 tgcccaacag cgttaaggac gatgcctcgc gtgcattatg gaaagctgag atgctgcgct
    24481 tacaaaaagc ggtagaggaa cgttttggac acgagattag cgaagatgct ctgcgcgatg
    24541 ccattgcgct gaaaaaccgc gaacgtcgcg cactggctaa tttttatcat cttgggcagt
    24601 taaatcctcc ggcgcttagc ggcagcgaca ttctgaaagt ggtttacggc gcaaccttcc
    24661 ggttcgataa agaggcgttg gttgacgaac tggatgcaat gaccgcccgc gttcgtcagc
    24721 agtgggaaga aggccagcga ctggacccgc gtccacgcat tttaatcacc ggctgcccaa
    24781 ttggcggcgc agcagagaaa gtggtgcgcg cgattgaaga gaatggcggc tgggttgtcg
    24841 gttatgaaaa ctgcaccggg gcgaaagcca ccgagcaatg cgtggcggaa acgggtgatg
    24901 tgtacgacgc gctggcggat aaatatctgg cgattggctg ctcctgtgtt tcgccgaacg
    24961 atcagcgcct gcaaatgctc agccagatgg ttgaagaata tcaggtcgat ggcgtagttg
    25021 atgtgatttt gcaggcgtgc catacctacg cggtggaatc gctggcgatt aaacgtcatg
    25081 tgcgtcagca gcacaacatt ccttatatcg ctattgaaac agactactcc acctcagatg
    25141 tcgggcagct cagcacccgt gtcgcggcct ttattgagat gctgtaagga gtggcagtgg
    25201 catattcgat tggcattgat tccggctcaa ccgccaccaa agggatctta ctggcagacg
    25261 gcgtgattac gcgccgtttc ctcgttccaa caccctttcg cccggcaaca gcaattactg
    25321 aagcctggga aactctgcgc gaagggttag aaacaacgcc gtttctgacg ctcaccggct
    25381 acggacggca actggtggat tttgccgata aacaggtgac ggaaatctcc tgtcacgggc
    25441 tgggcgcacg gtttcttgcg ccggcaacgc gcgcagtaat cgacatcggt ggtcaggaca
    25501 gcaaagtgat tcagcttgat gatgacggta acctgtgcga tttcctgatg aatgacaaat
    25561 gcgcggcggg caccgggcgt ttcctggagg tgatctcgcg cacgcttggc accagcgtcg
    25621 agcaactcga cagcattact gaaaatgtca gaccgcacgc catcacgagt atgtgcacag
    25681 tgtttgccga atcagaagtg atcagcctgc gctcagcggg cgtcgcgcca gaagcgattc
    25741 tcgctggagt gattaacgcg atggcgcggc ggagtgccaa tttcattgct cgtctctcct
    25801 gtgaagcgcc gattctgttt actggtggcg ttagtcattg ccagacgttt gcccggatgt
    25861 tggaaactca cctgggaatg ccggtaaata cccatcctga tgcgcaattt gctggcgcaa
    25921 ttggcgcagc ggtaattggt caacgagtga ggacacgccg atgaaagagt ttttattttt
    25981 atttcactcc acggtcggcg tcatacaaac ccgcaaggcg ttgcaggcag cgggcatgac
    26041 ttttcgcgtc agcgatattc cccgtgattt acgcggcggc tgcgggttat gcatttggct
    26101 gacctgtccc cccggcgagg aaatacaatg ggtgatccct gggcagaccg aagcgatata
    26161 ttgccagcag ggcagcgact ggcagtgcgt ggtgcattac gattcagaag cgtcaaccag
    26221 gcaataagac ctttaaatat aacgcccgga tatttggctg cacataatcc tgcatgaagg
    26281 aagttgtatt aaatatctcg cctgatgtga atgatgtgaa atatttccca cccacaaaat
    26341 gagcgatttt tgatagcacc ttactattgc gcgtctaaaa tacaacatac attgtattat
    26401 acttaaaccc ataattatac atagagcaat aaaatgatta ccgggtgtgg taatgataaa
    26461 gataagtttc agtgtgaagc ttatctttac ccacaccccc tgattatttt agatttagaa
    26521 gaacatttcg caattcaagg ataaaattat ggactcaaca tttaacatat accaggatat
    26581 tctcccagca ttcaacatgt attcgggact aaaaccttgc catgaaaaaa ataaccaacc
    26641 atttgatatt aacacggaaa ttgaaaccat acaaaaacaa attaattatg atataaatca
    26701 tttgaatgac ggtttgatta agcgtgtact gaatcttttt attcacctta tctctaatcc
    26761 cgataatctc gaattaacct taaatcgata ttcatcaaca acagagcaaa tcatcggcag
    26821 aaccaaaaga aatagtttac atgagtttga agttggcgat ctaaaaataa tatttaatcg
    26881 acaagatgat aatgaaagcg tattaactat taaacacaaa gatataagtc atggttgcaa
    26941 tgttaaaacc gagcaactgc agcagtttat taaaataatg gaacaaaaag cgcaactacc
    27001 aatctatatt gacaagaaca atttgaaaga gagtattttc tctgttttgc gaaatgaccc
    27061 acagcacgta gataaagagc aataccttcc ctgtgataag tttttaacac atgcctgcaa
    27121 aaattcaaat tcatttgaag tgaaattaga tgccactcat caatatcaac atctgaataa
    27181 cttcatgatt tcttttgacc cagtagaaaa tcaattaaca atacgggata acaataacga
    27241 gactgaaact atctcgttga caaacttaca atgggaaaat gtgctgcaat actacagaga
    27301 aaaccaccag cagccaaata tagcaggatc acgaaatctc acggataata tagataaaat
    27361 taaaaataca atatccacct ctgaaattat agagtgcgcc tctcctgaaa taagaagtag
    27421 cgtcctgaac gatctttata gcattgctaa tttcctcccg gacaaaaatc tgacaccaaa
    27481 tgagagctgg aaaagatttt gccatacatg cgagcgcttt tatgttgctc agaagagtat
    27541 cactggagat aacagtgaac gtcttacgcg aaaagtctct atctctgatg caggaattac
    27601 aatgaccttc aagataggtg atgttgtcat caatactatt agcactgcta tccctgaaga
    27661 tgaatcgggt caacggtgta tagaagggtt gaatttagca gagatggatt taaccggcat
    27721 agacttgtcg aaaatggcgc taaggaatgt caattttaat ggcagcattc ttagaaatgc
    27781 ggatttctcc ggtacgatct gtgaaggcgt ggattttacc gattgtgatc tccgttatgc
    27841 aacctttatt gacgcctcat tagaaaaaat cgattttcgt aaagttcgcc acttgtttaa
    27901 tataaatttt acaaatgcaa atctacggaa cagcaacttc agcggaaaag ttctcactgg
    27961 cgttaatttt actggaagtg accttagtaa cgcttatctt gaacacatag atttcacaaa
    28021 agtgattttc tttccgtcat taattattgg agcagtattt gataattcca atctatcaga
    28081 gaaaaatctt tcagacaaag atcttactaa tattagctgc atgtatacca attttactaa
    28141 cgctaattta acaaaatgca aactcttaaa tacaaacttt tcggctgcaa aatttgacaa
    28201 tactaatttc actggtacaa aaggttctaa tattctgttt aaccatgcat ggttgttcaa
    28261 tacgatattt atagatacga tttttaaaaa tgcctgtttt ttcaatgcca aagtgaataa
    28321 tgtttctctt aagaaagcat atatttacaa tgataatatc gataaaaaag ccaatgacag
    28381 taccgataaa caagccaaga acagtaccga gcaacaggac agtaccagtt ttaatcaagc
    28441 ccgtttaaag aaagaagtga atagcagttt ttccattccg ggtttaacgt cttatcagcc
    28501 aacgtatata gttgacgagt agttcaataa tcacccaaac agagtgagta tggcagagga
    28561 tataaattgt taaccgcctc atctatcatt catggatgag gcgcatttat cagtcgatgt
    28621 aagctgaaaa tgttctgcca tccagagaca accgccgccc cctgcggcgt ttactatttc
    28681 atcgcccgcg ttaagaattc attaagcttc atccgtatac tccttatctg tcgcactgaa
    28741 tttgacgaga aaaaagcaat aataaggagc aacaagttga aaaagcgcat cacctttatc
    28801 gtttttcttt gcgccatcgt ggcggtaagc ctgttttttg tgcaatcctg cgtccgtaaa
    28861 agccagcatg tcgcagggtt tcagaattat caggccacta tcgatggtaa agagattaca
    28921 ggagtaacta aaaatatctc ctcactgacc tggtctgcgc agagcaatac cttatttagc
    28981 actatcaaca aacctgcaac catcgttgaa atgacgaccg agggtgacct gatccgcacc
    29041 attccgctcg attttgttaa ggatctcgag acgattgaat acattggtga taataaattt
    29101 gtcatcagtg atgaacgtga ctacgccatt tatgtgattt cacttaacgc tgagtcggaa
    29161 gtaagcatcc tcaaaaaaat taaaatccca ttgcaggaaa caccaactaa ctgtggcttt
    29221 gaagggctgg cttactcctc tcaggaccat accttctggt tttttaaaga gaaaaatccg
    29281 atagaggtct acaaagtaac cgggctattg cgtagcgatg agttgcatat cagcaaagat
    29341 aaagcgttgc agcgtcagtt tactttagac gatgtttctg gcgcggaatt taacccacag
    29401 aaaaatacgt tgctggtgtt gtcacatgag tcacgcgccc tgcaggaagt gaccgtgaca
    29461 ggtgacgtga tcggcgaaat gtcgttaacc aaaggcaggt atggcctttc acacaatatc
    29521 aagcaggcgg aagggatagc gatggatgcc agtggcaaca tctatatcgt tggcgaaccc
    29581 aatctttttt atcgctttac gtcgacaaaa tcacgataac agtctggcag gttctccttt
    29641 tttgttatta acttgttaac tcaaaataat acaaacaagg aagaacatga attcgacatc
    29701 gcatcccgta gaacgttttt ctttcagcac cgcgttattc gggatgctgg ttctgacctt
    29761 aggtatgggt ttaggccgct ttctctatac gccgatgctg ccagtcatgc tggcggaagg
    29821 cgaattttcc tttagcgaac tctcatggat cgccagtggt aactatgccg ggtatctggc
    29881 ggggagcctg ctgttttcat tcggcgcgtt tcatttaccc tcacgcctgc gcccgttctt
    29941 gttagcttcc gccctcgcaa ccggattatt aatcctcgcg atggcgtggc tgccgccgtt
    30001 tcttctggtt ttcatcattc gctttctggc gggggtcgcc agcgccggga tgttgatttt
    30061 cggctcaacg ctcatcatgc agcatactcg tcatcccttt gtccttgcgg cgctattttc
    30121 tggtgttggc gtcggcatcg ctctgggcaa tgaatatgtg ctggcaggcc tgcattttgc
    30181 cctctcttca caaacgttgt ggcaaggtgc cggagcactt tctgccatta tattgcttgc
    30241 tctggcgctg ctcatcccgt cgaataaaca cgttatcccg ccagcgccat tggcaaaaat
    30301 cgcgcaacaa cccatgagct ggtggttact ggcgattctg tatggtctgg cgggttttgg
    30361 ttatatcatc gtcgccacct acctgccgct catggcgaaa gacgcgggcc agcctgtgct
    30421 gacggctcac ctctggacac tggtcggctt gtcgattgtc ccaggttgct ttggctggct
    30481 gtgggcagcc aaacggtggg gagcattacc ttgcctgacc gcgaatttgc tggtgcaggc
    30541 gatctgcgtg ctgttaaccc tcgccagcag ctctccttta ttactcatca tcagcagtat
    30601 tggttttggc ggcaccttta tgggaacgac ctcgctggtg atgaccatcg cccgccagct
    30661 tagcgtgccg ggaaatctta accttttggg ctttgtgaca ctcatttatg gtatcgggca
    30721 aattcttggc ccggcgctga ccagtatgct cggcaacgga acgtcggccc tcgccagtgc
    30781 cacgctctgc ggcgcggcgg cgctatttat cgcagcatta atctgcggga tgcaaatatt
    30841 caaattgcat acgaatgatt cttaagtaat ttaacgaatt taacttttca gtacatcacg
    30901 taaaataatt aaaacatcct ttttctttgg aggtactcag ccaccgccgt tattaatcct
    30961 ggtttagcaa ttccacggct aattcattat ttctggtcat attatagtca ggatcgcatt
    31021 atatctccgt gctagaaaaa acaagtttaa ttctgttttt tcacggaagg tcactatggc
    31081 ttcaacaaat gcgcatcaaa taaaaaataa tgatcaaaac tctttatgtg gtctgggtga
    31141 caaaatacgc cgcctgaccg ccggagtttg tttatttaca caaatttttt tccccgtcat
    31201 ggcgacggca caaaatgtgg tacacgctaa gcctcagacg acagtttcat ctccgtcccc
    31261 tctaatagaa aataatactg tgccttacac gcttggtgcg ctggaatcag cgcaaagcgt
    31321 tgctgatcga ttcggtattt cactggagga gcttcgtcgt cttaatcagt tccgtacttt
    31381 tgctcgcggc tttgataacg tgcgccaggg tgaagagctg gatgttccgg caacaacctt
    31441 gcagaaaagt catgagcaac aaaatgccgt accgcctgcg aatggcgaaa acacgctgga
    31501 gaatcaaata gccagcactt cgcagcgagt tggccccctg ctttcacaag atatgaatag
    31561 tgagcaggcc agcggcatgg cgcgtggttg ggcgtcttca gaagcctcag gcgcgatgac
    31621 tgattggtta aataactttg gcactgcgaa aatctctctg ggtgtggatg aagatttcag
    31681 cctgaaaaat tcgcaattcg acttcctgca tccgtggtat gacacacccg attatctgct
    31741 cttcagccag catacccttc accgaacaga cgatcgtacc cagatcaaca ccggtttggg
    31801 ctggcgtcat ttcaccccca gctggatgtc aggcatcaac ctgttttttg accacgacct
    31861 gagccgctat cactcccgcg cggggcttgg cgcagaatac tggcgtgatt atctgaagtt
    31921 gagcagcaac gcttatatcg gcctgaccgg ctggcgtagc gcaccggaat tggataatga
    31981 ctacgaagcc cgcccggcca acggctggga tttacgcgcg gaaggctggt taccagcctg
    32041 gccacaactg ggtggaaaac tggtctatga acaatactat ggtgatgaag tggcgctgtt
    32101 tgacaagaat gatcgccaaa gtaatcccca tgctattacc gcgggcctca actatacccc
    32161 cttcccgctt ctgactctca gtgcggaaca gcgtcagggg aaacaaggtg aaaatgacac
    32221 acgttttgcc gttgatctga cctggcaacc cagcagttca atgcagaaac agcttaaccc
    32281 ggacgaagtg gccggacggc gcagtctggc cggtagtcgt tatgacctga ttgatcgcaa
    32341 caacaacatc gttctggaat atcgcaagaa agagctgatt cacctgagtc tgcaggatcc
    32401 ggtgaaagga aagtctggag aaataaaacc gctggtttcc tcgatacaga ccaaatatgc
    32461 cctgaaaggc tataacatcg aagccgctgc gctggaagct gccggaggta aagtcaggac
    32521 gtctggaaaa gatatcacgg tcacgctgcc aggttaccgc ttcactaaca ctccagaaac
    32581 cgataataca tggtcgatag acgttaccgc cgaggatgta aaaggtaacc tgtcacgtca
    32641 tgaacaaagc atggtcgtca ttcaggctcc gacattaagc cagaaagatt ctctgttatc
    32701 cgttaatccg ctaaccgtag ctgcagataa aaaatcgacg accacattga ccgttactgc
    32761 acacgattcc gacggaactc cggtgccggg gctggcgctg caaacccgca gtgaaggcgt
    32821 tcaggatatc accctgtctg actggacaga taacggagat ggtagttaca cacagatgct
    32881 gaccgccgga acgacatcag gttcagtaac actgacgccg caaattaacg gtgagagtgc
    32941 ggtaaaagaa tccatcgtcg ttaatatcgt ccctgttgtc tcatcccgcg accattcatc
    33001 aataacaata gataacgtat cgtattatgc cggagacgac atcaaggtta gggtggaact
    33061 gaaagacgac agcaatcaac cggttgctta tcaaaaagaa gaattggtaa aagccgttac
    33121 tgtcgaaaac agcaaacctg gcgccacgat tgtctggcac gaagagcagc cgggcgttta
    33181 tgccgcgaat tatccggccc ataagcaagg gacagcacta agggcacaac ttagccttca
    33241 caactggaat gctccactgc aatcgcatat ttataacatt gaggcaaacc agaataaggc
    33301 tcgcgtcgcc acattatcag cgacaaataa tgacgtttac gccgataaaa agacatttaa
    33361 taccctcacg atcaacgtca ctgatgagag tgataatccc ctgacaaatc atcaggtcac
    33421 ctttaagaat gaaaaaggaa gcgctgaatt tgtcgaaccg ccgcagcaaa atacggatgc
    33481 atatggtgtt gccacaatca acatggtaag tcaggttgcg gaagaaaata cgattagtgc
    33541 cacgctgcca aatggtttct cacaacggat aattgcgaaa ttcgttagcg attcgagtac
    33601 gccaaaattc aaacaactgg ttgccgatcc agataccatt attgctggca acagccaggg
    33661 cagtactctg accgccaccg tcacagactt tcataacaac ccgttaaaag atatgaaagt
    33721 gaattttgtg gcacctggtg gctcgcaact ggacaacacg accgccacaa cagaccagtc
    33781 cggtattgtg cgagtgcacc tgaccagttc aaaagctggt agctattccg tcgatgcctc
    33841 gcttgaggcg gataaaaata ttcaccagtc ggtcacgatc accgtggtcc caaacaggga
    33901 acaatcggta atgaccttaa atgccgggtc gggcagtgcg atcgctaaca atacaaatac
    33961 cgttatcctg acagccagtg tgaaagatgt ttatggacac ccgttgccgg atgaggatgt
    34021 gaaatttacc ttgccagcct ccatgaccgg gaacttcacg ctaagtagtg agaccgcccg
    34081 caccgatgca aacggtgatg ccgtggttac attgcgaggc acaaaagcgg gtgagtttac
    34141 agttacggcg acgctgacca gaaataacac cgttgctcat cagcaagtca cttttattgg
    34201 ggatacaaac agtgcgcagc tccagccgct gactgcctca ttaaatacca ttgttgcggg
    34261 tgacagtacg gggagtaccc tgacggcaac gatcctggac gcttaccaaa atccgcttaa
    34321 agaccagctg gtcactttcc agagtaacga tgtcactcta agtggaacag aagtcaccac
    34381 caatacgctg ggtcaggcga cggtaacaat gaccagcaat attgccgggc aacataacgt
    34441 cgtggtgagc cggaaagcgc aagtctccga taataaaacg tttagtttat cagtgctgcc
    34501 ggatgaaagt tcggcgaagg taataagtat aaccggagcc gaaaaaacga taacggttgg
    34561 cgaaaacatc acgctacgga tactcgtcca ggacgcgttt aacaatgtaa tatcaggtca
    34621 acgcgtcaga ttaagtgcgc agccaacagc taacattacg ataggcgata cggcttacac
    34681 cgataataac ggttatgcat acgttaacct tattagcacc cagcctgggg tttatcaggt
    34741 gacggcaacg ctggacaata acagtagtag taaggttgac gtgaatgtgg caaatggcaa
    34801 gctcgagtta acatcatcga agccagaaac cacggtccat aacagtgaag gtattacgct
    34861 taccgcaacg gcgagaaatg cgcgggatga attgatgcct gggcaaatta tcacctttag
    34921 cgtaacgcct gaaggtgcaa cgctaagcaa tacaggggaa atccttactg accagtctgg
    34981 tcaggccaaa gtgacgctga ccagtaacaa agtgaatgtc tataccgtta cggccacaat
    35041 gggcaaagat gttcccgttc agagccaggt aacggttgcg gttaaggcag atgctaaaac
    35101 ggcacatgtt gtgagcgtcg tggcttctcc tgacaccata accgccgacg gcgttgatag
    35161 cagcaccatc acgtcacgag tagaagatga ttacggattc ccggttgaag gtgtcgatgt
    35221 tagttatgcc ttagacacca aaggcagccc ggtagttaat attccaacta cgcgtaccga
    35281 tcagtccggg caagtcacgg cgacaataac cagtacattg gcagaaacct taatagtcaa
    35341 tgtgcaagtt cctggtacag ccaaccaatc cgcaaccatt acattggttg ccggcacggc
    35401 cgatgaaagt aagtcaattt tgaaatccga cgttgacact ctgaaggctg actaccagca
    35461 gagcgcaaaa cttacgctaa cattgcaaga caagtacggt aacccgatag tgacgtctga
    35521 tcatctggaa tttgtccagt caggcccctt cgtgaacttt ctcaagttga gcgatattga
    35581 ttacagccaa agaaattatg gcgagtacac cgtgactgtc actggcggaa aagagggaac
    35641 agcgactctc attcccatgc tgaacggggt tcatcaggca aacttaagcg tatcgctgaa
    35701 tctcatccgc tcgataaaag aaatgtccgg tcatgtcact gcaaacaacc ataccttctc
    35761 cacggctaaa ttcccgagcg aaggttttgc aggagcgtat tacacactca acaatgataa
    35821 ctttgaagcg ggtaaaaccg ttgatgatta tatgttttca agttcacaga gttgggtgtc
    35881 ggtcgatgcg tcaggtaagg tttctttcgc aaatatcggc gatcaaacgt cagtcacaat
    35941 aagcgctgtt ccccgacaag gaggtacaac ctaccagacc ttaattaagc tgaaaggctg
    36001 gtgggtgaat aatggaaatc ataccaatat ctggctagct gccaatgcgc tctgtcatgc
    36061 taaaaatgat ggatatactc ttcctgccat cacacatttg acgtctggag aaaaccaacg
    36121 cacacaggga tcgctatatg gtgaatgggg gaacgttgga gcgttttcca gtaattcgca
    36181 atttacacaa ggtgcttact ggacaagtga atctgatgat tacaatcggc actactatgt
    36241 gcagatgcta acgggtatga ccggaagcga cgctgattcc agcccccaac tgaccgcctg
    36301 ccgtaaatca ctttaatttc agcaggttat taaggatacc cttgtgcgag ggtatccttt
    36361 ccgactgata acaaacgcta cattgcctga tccgctgcgc ttatcagtcc tatataaccc
    36421 ctgcaatatc ttgaatttat tgtttttgta acccagccgg catgaacggc cactcaagaa
    36481 actcaacagc aaccgttgat gtcagccaga ccccgttttc cgcttgccag aaagggaggc
    36541 ctcgtttcgc catttcctgt gctttgacgg ttaaaataac cggcgatccg tgtcttgctc
    36601 ccactttgcg tgccgttgct tcatcagcag agagatgtac gtagtgacgc tcacctgcaa
    36661 tcagcccctg tttttttatc tcgtcaagga aacggcttgc cgtaccgtgg tagagaaact
    36721 gtggcggtgt tttttcggca aaggaaatag cgacctgcga agtcgaatgc ccctgaaccg
    36781 cgcggatgca cctaccatca ctggaataac taaaacgctt tttatcgctg gtggcgacaa
    36841 cggtatccag caatgcacgg gttaaccgct tgcccgcttt ttgggcacaa agtatgagct
    36901 tatcgatatc cgcccatcct tcacggtcca gtacgatacc aatcgactcc ggtttgtgtc
    36961 ggaggacaaa acttaaaaac ttgctcgtat ccgccagctc tttttcgttg tactttgcca
    37021 tctcgttttc gtccctggat tttctgtttt tatttactta tgtcattgtc actgggttta
    37081 tgaatttact gcaattcaat ccagcgcaga accttttccc tatagcgtaa tttttccgct
    37141 atgcaaaaaa cgcatagcca gaaacatact tgcattttcc ccatacccac atggctgata
    37201 aggtttttca gctccaggaa ttttcctggc tcaagcaaca cacaaacaca aaaaaacaat
    37261 ggggattgtt atgacacaac aaggggatgc tgtagccggg gagctggcga cagaaaaagt
    37321 gggtattaaa gggtatctgg ctttttttct caccataatc tttttttccg gtgttttttc
    37381 cggaacagac agctggtggc gcgtttttga tttttccgtt ctgaacggat cgtttgggca
    37441 actgcctggt gcgaacggag ccaccacctc gtttcgcggg gcgggtggcg cgggggctaa
    37501 agatggcttt ctctttgcgc tggagctggc accgtcagtc attctctctc tgggaattat
    37561 ctcgattacc gatggtcttg gcggattgcg cgccgcgcag cagttaatga cgcccgtgct
    37621 aaaaccgttg ttgggcattc cagggatctg ctcgctggcg ctgatcgcta acctgcaaaa
    37681 caccgatgcc gccgccggta tgaccaaaga gctggcgcag gaaggtgaaa ttaccgaacg
    37741 tgataaagtg atattcgccg catatcaaac cagcggtagc gccattatca ccaactactt
    37801 ctcttccggc gtcgcggtgt ttgcctttct gggcacgtcc gtaatcgtac ctttagccgt
    37861 catcctggtg tttaaatttg tcggagccaa cattttgcgc gtctggctta acttcgaaga
    37921 acgtcgcaac ccaacgcaag gagcgcaagc atgacaactc aggtccgcaa aaacgtcatg
    37981 gatatgttta tcgatggcgc gcgccgtggc tttactatcg ccaccaccaa cctgctgcca
    38041 aacgtggtga tggcgtttgt catcattcag gcgctgaaaa ttaccggcct gctcgactgg
    38101 gtggggcata tctgtgaacc ggtaatggcg ctgtggggat tgccaggtga agcggcaacc
    38161 gtccttttag ccgcattaat gagtatgggc ggcgcagtcg gtgttgccgc cagtctggcg
    38221 accgctggcg cattaaccgg ccacgatgtc accgttttac tgcccgcgat gtatttaatg
    38281 ggcaacccgg tgcaaaatgt cggtcgctgc ctggggacag ccgaagt
//
LOCUS       DAMZFT010000031        37114 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.031, whole genome shotgun sequence.
ACCESSION   DAMZFT010000031 DAMZFT010000000
VERSION     DAMZFT010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 37114)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37114)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37114
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.031"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(<1..1030)
                     /locus_tag="PN977_001724"
     CDS             complement(<1..1030)
                     /locus_tag="PN977_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001226973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl CoA:acetate/3-ketoacid CoA transferase"
                     /protein_id="HDI6041766.1"
                     /translation="MRKITTAEALAAQIQDGATIAISGNGGGMVEADHILAAIEARFL
                     QTGHPRDLTLIHSLGIGDRDSKGTNRFAHAEMLKRIIAGHFTWSPKMQELVKSNAIEA
                     YCFPGGVIQALLREIGAGRPGLFTHVGLGSFVDPRNGGGKSNECTTTDLVELIEIDGE
                     TKLRYRPFKVDYAILRGTYADPRGNVSLEEEAIDMDSYSMALAAHNSGGKVFVQVRDV
                     LEAGAIEPRRVKLPGILVDGIVEHREQPQTYLGGYDLTISGQHRRLSSNDAIELVSHP
                     VRRLIARRAARELVAGASTNFGFGIPGGIPGVALREGVPYQSLWLSVEQGVHNGMMLD
                     DALFGCARN"
     gene            complement(1040..1816)
                     /locus_tag="PN977_001725"
     CDS             complement(1040..1816)
                     /locus_tag="PN977_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001283531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HDI6041767.1"
                     /translation="MSDQPVLFSRAAASCRLTLNREDKCHAINEEMIESLDHYLNEIE
                     NDTTLRLVELTATGDKFFCAGGDIKSWSAYSPLDMGRKWIKRGNDVFNRLRNLPQLTV
                     ANLNGHTIGGGIELALCCDIRIARPSAKFSNPEVMLGMVPGWMGIERVLNQVGPVVGR
                     QMLMLGKRLTAQEAQAANLIDEVVEKEQVESWMANQLAQLEKCGPVALAHIKQLILAL
                     ENKHADYPHQLLAGLMSATQDCQQATRAFAEKSSVSFHNQ"
     gene            complement(1826..2668)
                     /locus_tag="PN977_001726"
     CDS             complement(1826..2668)
                     /locus_tag="PN977_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001205218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HDI6041768.1"
                     /translation="MRDLLQRPDLFSINTATLGYKTPLPAIIDACAARGIGAIAPWRR
                     ELQGEDLQQIARQLAASNINVSGLCRSTYYTAPTLAERKLAIDDNRRALDDAAVLNAA
                     CYMQVVGGLPMGTKDLYEAREQVKQGIRQLLPHSKDVGVPIALEPLHPMTAADRSCLC
                     TLRQALDWCDELDPDGEFGLGVAVDVYHVWWDPDLVSQILRAGKRILAFHVSDWLVPT
                     TDLVNDRGMPGDGVINIPSIRRLVENAGFNGAIELEIFSPYWWQQDIDSTLDISVDRI
                     AHYC"
     gene            complement(2678..3469)
                     /locus_tag="PN977_001727"
     CDS             complement(2678..3469)
                     /locus_tag="PN977_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-ketoacyl-ACP reductase"
                     /protein_id="HDI6041769.1"
                     /translation="MKSTRPVAVITGAARGIGKGCALELARSGFNLLINDRPDADSVE
                     KLHITQQECMAEGVEVICFPADVGDLSLHEEMLDAAQNQWGRLDCLLNNAGISVKKRG
                     DLLDLEPDSFDQNIAINTRAPFFLAQAFSKRLLAQPKPEAELPHRSIIFVSSINAIML
                     AMNRGEYTIAKTAVSAAARLFAARLCNEQIGVYEVRPGLIKTDMTIPATAYYDELIAK
                     GLVPWGRWGYPADIASTVRAMAEGKLIYTCGQAVAIDGGLSMPRF"
     gene            3679..4317
                     /locus_tag="PN977_001728"
     CDS             3679..4317
                     /locus_tag="PN977_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002431731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HDI6041770.1"
                     /translation="MYLDTSHAQNLKEKLLLCAVNEFAEYGYEGARVDNIVKAAGCSK
                     QTVYHHFGNKENLFIEVLEYTWNDIREKERELDISGLSPVIAIEKIIDFTWDYYINNP
                     WFLKIVHSENQSKGVHYKKSKRLAEINHAHLQSMASLLEEGQRQKTFKKYIDPLQINI
                     NIAALGAYYLINQYTLGMVYHISMVSPEALEARRRVIKETILSWLLVNPARG"
     gene            complement(4318..4947)
                     /locus_tag="PN977_001729"
     CDS             complement(4318..4947)
                     /locus_tag="PN977_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OapA family protein"
                     /protein_id="HDI6041771.1"
                     /translation="MPGRFELKPTLEKVWHAPDNFRFMDPLPPMHRRGVIIAAIVLII
                     GILLPGDETRQMPVVTREAQLDMQSPPPGEAQLQAHLVTPQNDPDQVAPVAPEPIQEG
                     QPEEPPVAETQSFQPESGIDQQWRTYRVEPGKTLAQLFRDHNLPPTDVYAMAQVEGAD
                     KPLSNLQNGQIVKIRQNANGVVTGLTIDTGNNQQVLFTRQPDGNFIRAR"
     gene            5165..5785
                     /gene="fklB"
                     /locus_tag="PN977_001730"
     CDS             5165..5785
                     /gene="fklB"
                     /locus_tag="PN977_001730"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015742582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HDI6041772.1"
                     /translation="MTTPTFDTIEAQASYGIGLQVGQQLSESGLEGLLPEALVAGIAD
                     ALEGKHPAVPVDVVHRALREIHERADAVRRQRFQAMAAEGVKYLEENAKKEGVNSTES
                     GLQFRVINQGEGAIPARTDRVRVHYTGKLIDGTVFDSSVARGEPAEFPVNGVIPGWIE
                     ALTLMPVGSKWELTIPQELAYGERGAGASIPPFSTLVFEVELLEIL"
     gene            6094..7497
                     /gene="cycA"
                     /locus_tag="PN977_001731"
                     /gene_synonym="dagA"
     CDS             6094..7497
                     /gene="cycA"
                     /locus_tag="PN977_001731"
                     /gene_synonym="dagA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine/D-alanine/glycine transporter"
                     /protein_id="HDI6041773.1"
                     /translation="MVDQVKVVADDQAPAEQSLRRNLTNRHIQLIAIGGAIGTGLFMG
                     SGKTISLAGPSIIFVYMIIGFMLFFVMRAMGELLLSNLEYKSFSDFASDLLGPWAGYF
                     TGWTYWFCWVVTGMADVVAITAYAQFWFPGLSDWVASLSVIILLLVLNLATVKMFGEM
                     EFWFAMIKIVAIVSLIVVGLVMVAMHFQSPTGVEASFAHLWNDGGWFPKGLSGFFAGF
                     QIAVFAFVGIELVGTTAAETKDPEKSLPRAINSIPIRIIMFYVFSLIVIMSVTPWSSV
                     VPEKSPFVELFVLVGLPAAASVINFVVLTSAASSANSGVFSTSRMLFGLAQEGVAPKA
                     FAKLSKRAVPAKGLTFSCICLLGGVVMLYVNPSVIGAFTMITTVSAILFMFVWTIILC
                     SYLVYRKQRPHLHEKSIYKMPLGKLMCWVCMAFFVFVLVLLTLEDDTRQALLVTPLWF
                     IALGLGWLFIGKKRMAK"
     gene            7631..8788
                     /locus_tag="PN977_001732"
     CDS             7631..8788
                     /locus_tag="PN977_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016246814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6041774.1"
                     /translation="MVKRSWLLIAALPPSISPSWGADFYYRQQEKGTVYVVEQKGEKD
                     EILSELPDINFSRLWRIANLANKQDSRLLSDFNPDKFDCDDEGDCEHAWLTDGRSVLW
                     SGKVLKNPPGKPIVDAASFQAFGAFAADKRSIYFDGQRTDDNSGDKQVDMSTLEETDI
                     WNLLRDKNSLWHKGHWLGSADGFQILRHDSSLQFVVQTNSQVIVNGKPLPADRKTFQI
                     KRWMPGERLVYRDKSGERDYELEDTSYRCAPFNIGLNNVSWLKYEATPAGSECIYETL
                     AGVDPEYFYLFVRNTGLYKNQIYKVTINALGEGELVNLKPEDLSDSLEAGGSWGLTNT
                     FISTDGQLYTQQATGIGKEHAQQGEWLRYNLGKGGWLSVKQPPSGLKPLFK"
     gene            8821..10314
                     /locus_tag="PN977_001733"
     CDS             8821..10314
                     /locus_tag="PN977_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000936770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6041775.1"
                     /translation="MLPIKPVCLALALFSLNSIACSGVPSASFYYQPWGQGTKYIVED
                     SDGYGLYFPDIHLPQVRFKQLRRVSDGTDDEYDSPDDINNDFVRVDGGAYIRTNGGWR
                     FDWLTDGRHILWAGKIVQNPPGTPKVDAATFRAWGRFAADKDSLYFDGERTDDNHGEK
                     QVDMDSLQQVGGRMNSLDSADVLKDRRNLYFQGRWLGSAQGYSILDIKPWTRRPIIYS
                     PNSCESKSNPGPWDTIWRTKSQVFVNGDAINADPDTFHIVRWIAGSLLIYRDKDGEKR
                     YAFGKNCRSIFDLQRDKVTWMTRDASRRANDCRVATIPDVDPEYFRPLTENVAQYKDS
                     LYLVKYISAVEKTLSVIHLPDPQQELQKGANVVGNKIYFIARDDVTIFDINGQWQWYK
                     SPDGTPLNYLAHDDRYTYFIDEDTVEHFELKGQWTWFKYANGQLSDTFAHDDHYIYYV
                     GDGLVRDAKRRNETRQLDAAHLDKNGSLLTVEGKYTSYNNELLPLND"
     gene            complement(10359..11021)
                     /gene="ytfE"
                     /locus_tag="PN977_001734"
     CDS             complement(10359..11021)
                     /gene="ytfE"
                     /locus_tag="PN977_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster repair protein YtfE"
                     /protein_id="HDI6041776.1"
                     /translation="MAYRDQPLGELALSIPRASALFRKYDMDYCCGGKQTLARAAARK
                     ELDVDVIEAELAKLAEQPIEKDWRSAPLAEIIDHIIVRYHDRHREQLPELILQATKVE
                     RVHADKPSVPKGLTKYLTMLHEELSSHMMKEEQILFPMIKQGMGSQAMGPISVMESEH
                     DEAGELLEVIKHTTNNVTPPPEACTTWKAMYNGINELIDDLMEHISLENNVLFPRALA
                     GE"
     gene            complement(11129..12094)
                     /locus_tag="PN977_001735"
     CDS             complement(11129..12094)
                     /locus_tag="PN977_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418631.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HDI6041777.1"
                     /translation="MISGVLYALLAGLMWGLIFVGPLIVPEYPAMLQSMGRYLALGLI
                     ALPIAWLGRVRLRQLARRDWLTALMLTMMGNLIYYFCLASAIQRTGAPVSTMIIGTLP
                     VVIPVFANLLYSQRDGKLAWGKLAPALICIGIGLACVNIAELNHGLPDFDWARYTSGI
                     VLALVSVVCWAWYALRNARWLRENPDKHPMMWATAQALVTLPVSLIGYLVACYWLNMQ
                     TPDFSLPFGPRPLVFISLMVAIAVLCSWVGALCWNVASQRLPTVILGPLIVFETLAGL
                     LYTFLLRQQMPPLMTLSGIALLVIGVVIAVRAKPEKPLTESVSES"
     gene            complement(12202..13062)
                     /gene="qorB"
                     /locus_tag="PN977_001736"
     CDS             complement(12202..13062)
                     /gene="qorB"
                     /locus_tag="PN977_001736"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HDI6041778.1"
                     /translation="MIAITGATGQLGHYVIESLMKTVPASQIVAIVRNPAKAQALTAQ
                     GITVRQADYGDEAALTSALQGVEKLLLISSSEVGQRAPQHRNVINAAKAAGVKFIAYT
                     SLLHADKSPLGLADEHIETEKMLADSGIVYTLLRNGWYSENYLASAPAALKHGVFIGA
                     AGDGKIASATRADYAAAAARVISEAGHEGKVYELAGDSAWTLTQLAAELTKQSGKQVT
                     YQNLSEADFAAALKSVGLPDGLADMLADSDVGASKGGLFDNSKTLSKLIGRPTTTLAE
                     SVSHLFNVNN"
     gene            13151..13531
                     /locus_tag="PN977_001737"
     CDS             13151..13531
                     /locus_tag="PN977_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HDI6041779.1"
                     /translation="MSQVSLSQQLKEGNLFAEQCPSREVLKHVTSRWGVLILVALREG
                     THRFSDLRRKMGGVSEKMLAQSLQALEQDGFLNRIAYPVVPPHVEYSLTPLGEQVSEK
                     VAALADWIELNLPEVLAVRDERAA"
     gene            complement(13660..15603)
                     /gene="cpdB"
                     /locus_tag="PN977_001738"
     CDS             complement(13660..15603)
                     /gene="cpdB"
                     /locus_tag="PN977_001738"
                     /EC_number="3.1.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2',3'-cyclic-nucleotide 2'-phosphodiesterase"
                     /protein_id="HDI6041780.1"
                     /translation="MIKFSATLLATLIAASVNAATVDLRIMETTDLHSNMMDFDYYKD
                     TATEKFGLVRTASLINDARNEVKNSVLVDNGDLIQGSPLADYMSAKGLKAGDIHPVYK
                     ALNTLDYTVGTLGNHEFNYGLDYLKNALAGAKFPYVNANVIDARTKQPMFTPYLIKDT
                     EVVDKDGKKQTLKIGYIGVVPPQIMGWDKANLSGKVTVNDITETVRKYVPEMREKGAD
                     VVVVLAHSGLSADPYKVMAENSVYYLSEIPGVNAIMFGHAHAVFPGKDFADIEGADIA
                     KGTLNGVPAVMPGMWGDHLGVVDLQLSNDSGKWQVTQAKAEARPIYDIANKKSLAAED
                     SKLVETLKADHDATRQFVSKPIGKSADNMYSYLALVQDDPTVQVVNNAQKAYVEHYIQ
                     GDPDLAKLPVLSAAAPFKVGGRKNDPASYVEVEKGQLTFRNAADLYLYPNTLIVVKAS
                     GKEVKEWLECSAGQFNQIDPNSTKPQSLINWDGFRTYNFDVIDGVNYQIDVTQPARYD
                     GECQMINANAERIKNLTFNGKPIDPNAMFLVATNNYRAYGGKFAGTGDSHIAFASPDE
                     NRSVLAAWIADESKRAGEIHPAADNNWRLAPIAGDKKLDIRFETSPSDKAAAFIKEKG
                     QYPMNKVATDDIGFAIYQVDLSK"
     gene            15793..16533
                     /gene="cysQ"
                     /locus_tag="PN977_001739"
     CDS             15793..16533
                     /gene="cysQ"
                     /locus_tag="PN977_001739"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HDI6041781.1"
                     /translation="MLDQVCQLARNAGDAIMQVYDGTKPMDVVSKADNSPVTAADIAA
                     HTVIMDGLRTLTPDIPVLSEEDPPGWEVRQHWQRYWLVDPLDGTKEFIKRNGEFTVNI
                     ALIDHGKPILGVVYAPVMNVMYSAAEGKAWKEECGVRKQIQVRDARPPLVVISRSHAD
                     AELKEYLQQLGEHQTTSIGSSLKFCLVAEGQAQLYPRFGPTNIWDTAAGHAVAAAAGA
                     HVHDWQGKPLDYTPRESFLNPGFRVSIY"
     gene            complement(16523..17080)
                     /locus_tag="PN977_001740"
     CDS             complement(16523..17080)
                     /locus_tag="PN977_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001498728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HDI6041782.1"
                     /translation="MTLRKILALTCLLLPMMASAHQFETGQRVPPIGITDRGELVLDK
                     DQFSYKTWNSAQLVGKVRVLQHIAGRTSAKEKNATLIEAIKSAKLPHDRYQTTTIVNT
                     DDAIPGSGMFVRSSLESNKKLYPWSQFIVDSNGVARGAWQLDEKSSAVVVLDKDGRVQ
                     WAKDGALTQEEVQQVMDLLHKLINK"
     gene            17405..17611
                     /locus_tag="PN977_001741"
     CDS             17405..17611
                     /locus_tag="PN977_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1107 domain-containing protein"
                     /protein_id="HDI6041783.1"
                     /translation="MKIFQRYNPLQVAKYVKILFRGRLYIKDVGAFEFDKGKILIPKV
                     KDKLHLSVMSEVNRQVMRLQTEMA"
     gene            complement(17673..19016)
                     /locus_tag="PN977_001742"
     CDS             complement(17673..19016)
                     /locus_tag="PN977_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HDI6041784.1"
                     /translation="MLNSILVILCLIAVSAFFSMSEISLAASRKIKLKLLADEGNINA
                     QRVLNMQENPGMFFTVVQIGLNAVAILGGIVGDAAFSPAFHSLFSRYMSAELSEQLSF
                     ILSFSLVTGMFILFADLTPKRIGMIAPEAVALRIINPMRFCLYVCTPLVWFFNGLANI
                     IFRIFKLPMVRKDDITSDDIYAVVEAGALAGVLRKQEHELIENVFELESRTVPSSMTP
                     RENVIWFDLHEDEQSLKNKVAEHPHSKFLVCNEDIDHIIGYVDSKDLLNRVLANQSLA
                     LNSGVQIRNTLIVPDTLTLSEALESFKTAGEDFAVIMNEYALVVGIITLNDVMTTLMG
                     DLVGQGLEEQIVARDENSWLIDGGTPIDDVMRVLDIDEFPQSGNYETIGGFMMFMLRK
                     IPKRTDSVKFAGYKFEVVDIDNYRIDQLLVTRIDSKATALSPKLPDAKDKEESVA"
     gene            complement(19339..19977)
                     /gene="msrA"
                     /locus_tag="PN977_001743"
     CDS             complement(19339..19977)
                     /gene="msrA"
                     /locus_tag="PN977_001743"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HDI6041785.1"
                     /translation="MSLFDKKHLVSPADALPGRNTPMPVATLHAVNGHSMTNVPDGME
                     IAIFAMGCFWGVERLFWQLPGVYSTAAGYTGGYTPNPTYREVCSGDTGHAEAVRIVYD
                     PSVISYEQLLQVFWENHDPAQGMRQGNDHGTQYRSAIYPLTPEQDAAARASLERFQAA
                     MLAADDDRRITTEIANATPFYYAEDDHQQYLHKNPYGYCGICGIGVCLPPEA"
     gene            20183..21916
                     /gene="tamA"
                     /locus_tag="PN977_001744"
     CDS             20183..21916
                     /gene="tamA"
                     /locus_tag="PN977_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamA"
                     /protein_id="HDI6041786.1"
                     /translation="MRYIRQLCCVSLLCLSGSAVAANIRLQVEGLSGQLEKNVRAQLS
                     TIESDEVTPDRRFRARVDDAIREGLKALGYYQPTIEFDLRPPPKKGRQVLIAKVTPGV
                     PVLIGGTDVVLRGGARTDKDYLKLLDTRPAIGTVLNQGDYENFKKSLTSIALRKGYFD
                     SEFTKAQLGIALGLHKAFWDIDYNSGERYRFGHVTFEGSQIRDEYLQNLVPFKEGDEY
                     ESKDLAELNRRLSATGWFNSVVVAPQFDKSRETKVLPLTGVVSPRTENTIETGVGYST
                     DVGPRVKATWKKPWMNSYGHSLTTSTSISAPEQILDFSYKMPLLKNPLEQYYLVQGGF
                     KRTDLNDTESDSTTLVASRYWDLSSGWQRAINLRWSLDHFTQGEITNTTMLFYPGVMI
                     SRTRSRGGLMPTWGDSQRYSIDYSNTAWGSDVDFSVFQAQNVWIRTLYDRHRFVTRGT
                     LGWIETGDFDKVPPDLRFFAGGDRSIRGYKYKSIAPKYANGDLKGASKLITGSLEYQY
                     NVTGKWWGAVFVDSGEAVSDIRRSDFKTGTGVGVRWESPVGPIKLDFAVPVADKDEHG
                     LQFYIGLGPEL"
     gene            21913..25692
                     /gene="tamB"
                     /locus_tag="PN977_001745"
     CDS             21913..25692
                     /gene="tamB"
                     /locus_tag="PN977_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamB"
                     /protein_id="HDI6041787.1"
                     /translation="MSLWKKISLGVVIVILLLLGSVAFLVGTTSGLHLVFKAADRWVP
                     GLDIGKVTGGWRDLTLSDVRYEQPGVAVKAGNLHLAVGLECLWNSSVCINDLALKDIQ
                     VNIDSKKMPPSEQVEEEEDSGPLDLSTPYPITLTRVALDNVNIKIDDTTVSVMDFTSG
                     LNWQEKTLTLKPTSLKGLLIALPKVADVAQEEVVEPKIENPQPDEKPLGETLKDLFSR
                     PVLPEMTDVHLPLNLNIEEFKGEQLRVTGDTDITVSTMLLKVSSIDGNTKLDALDIDS
                     SQGIVNASGTAQLSDNWPVDITLNSTLNVEPLKGEKVKLKVGGALREQLEIGVNLSGP
                     VDMDLRAQTRLAEAGLPLNVEVNSKQLYWPFTGEKQYQADDLKLKLTGKMTDYTLSMR
                     TAVKGQEIPPATITLDAKGNEQQVNLDKLTVAALEGKTELKALLDWQQAISWRGELTL
                     NGINTAKEFPEWPSKLNGLIKTRGSLYGGTWQMEVPELKLTGNVKQNKVNVDGTLKGN
                     SYMQWKIPGLHLELGPNSAEVKGELGVKDLNLDATINAPGLDNALPGLGGTAKGLVKV
                     RGTVEAPQLLADITARGLRWQELSVAQVRVEGDIKSTDQIAGKLDVRVEQISQPDVSI
                     NLVTLNAKGSEKQHELQLRIQGEPVSGQLNLAGSFDRKEERWKGTLSNTRFQTPVGPW
                     SLTRDIALDYRNKEQKISIGPHCWLNPNAELCVPQTIDAGAEGRAVVNLNRFDLAMLK
                     PFMPETTQASGIFTGKADVAWDTTKEGLPQGSITLSGRNVQVTQTVNDAALPVAFQTL
                     NLTAELRNNRAELGWTIRLTNNGQFDGQVQVTDPQGRRNLGGNVNIRNFNLAMINPIF
                     TRGEKAAGMVSANLRLGGDVQSPQLFGQLQVTGVDIDGNFMPFDMQPSQLAVNFSGMR
                     STLAGTVRTQQGEIYLNGDADWSQIENWRARVTAKGSKVRITVPPMVRMDVSPDVVFE
                     ATPNLFTLDGRVDVPWARIVVHELPESAVGVSSDVVMLNDNLQPEEAKTASIPINSNL
                     IVHVGNNVRIDAFGLKARLTGDLNVVQDKQGLGLNGQINIPEGRFHAYGQDLIVRKGE
                     LLFSGPPDQPYLNIEAIRNPDATEDDVIAGVRVTGLADEPKAEIFSDPAMSQQAALSY
                     LLRGQGLESDQSDSAAMTSMLIGLGVAQSGQIVGKIGETFGVSNLALDTQGVGDSSQV
                     VVSGYVLPGLQVKYGVGIFDSIATLTLRYRLMPKLYLEAVSGVDQALDLLYQFEF"
     gene            25695..26036
                     /locus_tag="PN977_001746"
     CDS             25695..26036
                     /locus_tag="PN977_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HDI6041788.1"
                     /translation="MRIFVYGSLRHKQGNSHWMTNAQLLGDFSIDNYQLYSLGHYPGA
                     VPGNGTVHGEVYRIDNATLAELDALRTRGGEYARQLIQTPYGSAWMYVYQRPVDGLKL
                     IESGDWLDRDK"
     gene            26248..26499
                     /gene="chpS"
                     /locus_tag="PN977_001747"
     CDS             26248..26499
                     /gene="chpS"
                     /locus_tag="PN977_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin ChpS"
                     /protein_id="HDI6041789.1"
                     /translation="MRITIKRWGNSSGMVIPNVVMKELNLRPGQSVEAQVSNNQLILT
                     PISRRYSLDELLAQCDMNATELSEQDVWGKSTPAGDEIW"
     gene            26493..26843
                     /gene="chpB"
                     /locus_tag="PN977_001748"
     CDS             26493..26843
                     /gene="chpB"
                     /locus_tag="PN977_001748"
                     /EC_number="4.6.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endoribonuclease toxin ChpB"
                     /protein_id="HDI6041790.1"
                     /translation="MVKKSEFERGDIVLVGFDPASGHEQQGAGRPALVLSVQAFNQLG
                     MTLVAPITQGGNFARYAGFSVPLHCEEGDVHGVVLVNQVRMMDLRARLAKRIGLAAGE
                     VVEEALLRLQAVVE"
     gene            complement(26922..27452)
                     /gene="ppa"
                     /locus_tag="PN977_001749"
     CDS             complement(26922..27452)
                     /gene="ppa"
                     /locus_tag="PN977_001749"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001739810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HDI6041791.1"
                     /translation="MSLLNVPAGKDLPEDIYVVIEIPANADPIKYEIDKESGALFVDR
                     FMSTAMFYPCNYGYINHTLSLDGDPVDVLVPTPYPLQPGSVIRCRPVGVLKMTDEAGE
                     DAKLVAVPHSKLSKEYDHIKDVNDLPELLKAQIAHFFEHYKDLEKGKWVKVEGWENAE
                     AAKAEIVASFERAKNK"
     gene            27762..28718
                     /gene="ytfQ"
                     /locus_tag="PN977_001750"
     CDS             27762..28718
                     /gene="ytfQ"
                     /locus_tag="PN977_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter
                     substrate-binding protein YtfQ"
                     /protein_id="HDI6041792.1"
                     /translation="MWKRLLVVSAVSAAMSSMALAAPLTVGFSQVGSESGWRAAETNV
                     AKSEAEKRGITLKIADGQQKQENQIKAVRSFVAQGVDAIFIAPVVATGWEPVLKEAKD
                     AEIPVFLLDRSIDVKDKSLYMTTVTADNILEGKLIGDWLVKEVNGKPCNVVELQGTVG
                     ASVAIDRKKGFAEAIKNAPNIKIIRSQSGDFTRSKGKEVMESFIKAENNGKNICMVYA
                     HNDDMVIGAIQAIKEAGLKPGKDILTGSIDGVPDIYKAMIDGEANASVELTPNMAGPA
                     FDALEKYKKDGTMPEKLTLTKSTLYLPDTAKEELEKKKNMGY"
     gene            28851..30353
                     /locus_tag="PN977_001751"
     CDS             28851..30353
                     /locus_tag="PN977_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HDI6041793.1"
                     /translation="MNTNQHQEILRTEGLSKFFPGVKALDNVDFSLRRGEIMALLGEN
                     GAGKSTLIKALTGVYHADRGTIWLEGQTISPKNTAHAQQLGIGTVYQEVNLLPNMSVA
                     DNLFIGREPKRFGLLRRKEMEKRATELMASYGFSLDVREPLNRFSVAMQQIVAICRAI
                     DLSAKVLILDEPTASLDTQEVELLFGLMRQLRDRGVSLIFVTHFLDQVYQVSDRITVL
                     RNGSFVGCRETRELPQIELVKMMLGRELDTHALQRAGRTLLSDKPVAAFKNYGKKGMI
                     APFDLEVRPGEIVGLAGLLGSGRTETAEVIFGIKPADSGTALIKGKPQTLRSPHQASV
                     LGIGFCPEDRKTDGIIAAASVRENIILALQAQRGWLRPISRKEQQEIAERFIRQLGIR
                     TPSTEQPIEFLSGGNQQKVLLSRWLLTRPQFLILDEPTRGIDVGAHAEIIRLIETLCA
                     DGLALLVISSELEELVGYADRVIIMRDRKQVAEIPLAALSVPAIMNAIAA"
     gene            30367..31389
                     /locus_tag="PN977_001752"
     CDS             30367..31389
                     /locus_tag="PN977_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDI6041794.1"
                     /translation="MPQSLPDTTPSKRRFRWPTGMPQLAALLLVLLVDSLVAPHFWQV
                     VLQDGRLFGSPIDILNRAAPVALLAIGMTLVIATGGIDLSVGAVMAIAGATTAAMTVA
                     GFSLPIVLLSALGTGILAGLWNGILVAILKIQPFVATLILMVAGRGVAQLITAGQIVT
                     FNSPDLSWFGSGSLLFLPTPVIIAVLTLLLFWLLTRKTALGMFIEAVGINIRAAKNAG
                     VNTRIIVMLTYVLSGLCAAIAGIIVAADIRGADANNAGLWLELDAILAVVIGGGSLMG
                     GRFNLLLSVVGALIIQGMNTGILLSGFPPEMNQVVKAVVVLCVLIVQSQRFISLIKGV
                     RNRDKT"
     gene            31376..32371
                     /gene="yjfF"
                     /locus_tag="PN977_001753"
     CDS             31376..32371
                     /gene="yjfF"
                     /locus_tag="PN977_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease YjfF"
                     /protein_id="HDI6041795.1"
                     /translation="MIKRNLPLMITIGVFVLGYIYCLTQFPGFASTRVICNILTDNAF
                     LGIIAVGMTFVILSGGIDLSVGSVIAFTGVFLAKVIGDFGLSPLLAFPLVLVMGCAFG
                     AFMGLLIDALKIPAFIITLAGMFFLRGVSYLVSEESIPINHPVYDTLSSLAWKIPGGG
                     RLSAMGLLMLAVVVIGIFLAHRTRFGNQVYAIGGNATSANLMGISTRSTTIRIYMLST
                     GLATLAGIVFSIYTQAGYALAGVGVELDAIASVVIGGTLLSGGVGTVLGTLFGVAIQG
                     LIQTYINFDGTLSSWWTKIAIGILLFIFIALQRGLTVLWENRQSSPVTRVNIAQR"
     gene            complement(32404..33402)
                     /gene="fbp"
                     /locus_tag="PN977_001754"
     CDS             complement(32404..33402)
                     /gene="fbp"
                     /locus_tag="PN977_001754"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HDI6041796.1"
                     /translation="MKTLGEFIVEKQHEFSHATGELTALLSAIKLGAKIIHRDINKAG
                     LVDILGASGAENVQGEVQQKLDLFANEKLKAALKARDIVAGIASEEEDEIVVFEGCEH
                     AKYVVLMDPLDGSSNIDVNVSVGTIFSIYRRVTPVGTPVTEEDFLQPGNKQVAAGYVV
                     YGSSTMLVYTTGCGVHAFTYDPSLGVFCLCQERMRFPEKGKTYSINEGNYIKFPNGVK
                     KYIKFCQEEDKSTNRPYTSRYIGSLVADFHRNLLKGGIYLYPSTASHPDGKLRLLYEC
                     NPMAFLAEQAGGKASDGKERILDIIPETLHQRRSFFVGNDHMVEDVERFIREFPDA"
     gene            33578..34951
                     /gene="mpl"
                     /locus_tag="PN977_001755"
     CDS             33578..34951
                     /gene="mpl"
                     /locus_tag="PN977_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HDI6041797.1"
                     /translation="MRIHILGICGTFMGGLAMLARQLGHEVTGSDANVYPPMSTLLEK
                     QGIELIQGYDASQLDPQPDLVIIGNAMTRGNPCVEAVLEKNIPYMSGPQWLHDFVLRD
                     RWVLAVAGTHGKTTTAGMATWILEQCGYKPGFVIGGVPGNFEVSACLGESDFFVIEAD
                     EYDCAFFDKRSKFVHYCPRTLILNNLEFDHADIFDDLKAIQKQFHHLVRIVPGQGRII
                     WPENDINLKQTMAMGCWSEQELVGEQGHWQAKKLTTDASEWEVLLDGEKVGEVKWSLV
                     GEHNMHNGLMAIAAARHVGVAPADAANALGSFINARRRLELRGEANGVTVYDDFAHHP
                     TAILATLAALRGKVGGTARIIAVLEPRSNTMKMGICKDDLAPSLGRADEVFLLQPAHI
                     PWQVAEVAEACVQPAHWSGDVDTLADMVVKTAQPGDHILVMSNGGFGGIHQKLLDGLA
                     KKAEAAQ"
     gene            complement(35034..35585)
                     /locus_tag="PN977_001756"
     CDS             complement(35034..35585)
                     /locus_tag="PN977_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein"
                     /protein_id="HDI6041798.1"
                     /translation="MTKQPEDWLDDVPGDDIEDEDDEIIWVSKSEIKRDAEELKRLGA
                     EIVDLGKNALDKIPLDADLRAAIELAQRIKMEGRRRQLQLIGKMLRQRDVEPIRQALD
                     KLKNRHNQQVVLFHKLENLRDRLIDQGDDAIAEVLNLWPDADRQQLRTLIRNAKKEKE
                     GNKPPKSARQIFQYLRELAENEG"
     gene            35679..37031
                     /gene="pmbA"
                     /locus_tag="PN977_001757"
     CDS             35679..37031
                     /gene="pmbA"
                     /locus_tag="PN977_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease PmbA"
                     /protein_id="HDI6041799.1"
                     /translation="MALAMKVISQVEAQRKILEEAVSTALELASGKSDGAEVAVSKTT
                     GISVSTRYGEVENVEFNSDGALGITVYHQNRKGSASSTDLSPQAIARTVQAALDIARY
                     TSPDPCAGVADKELLAFDAPDLDLFHPAEVSPDEAIELAARAEQAALQADKRITNTEG
                     GSFNSHYGVKVFGNSHGMLQGYCSTRHSLSSCVIAEENGDMERDYAYTIGRAMSDLQT
                     PEWVGADCARRTLSRLSPRKLSTMKAPVIFANEVATGLFGHLVGAIAGGAVYRKSTFL
                     LDSLGKQILPDWLTIEEHPHLLKGLASTPFDSEGVRTERRDIVKDGILTQWLLTSYSA
                     RKLGLKSTGHAGGIHNWRIAGQGLSFEQMLKEMGTGLVVTELMGQGVSAITGDYSRGA
                     AGFWVENGEIQYPVSEITIAGNLKDMWRNIVAVGNDIETRSNIQCGSVLLPEMKIAGQ
                     "
BASE COUNT         8910 a         9217 c         9936 g         9051 t
ORIGIN      
        1 cgttacgggc gcagccgaac agagcatcat ccagcatcat gccgttatgt acaccctgtt
       61 ctacactcag ccacaaactt tgataaggca cgccttcgcg cagcgctacg cctggaatac
      121 cgcccggaat accaaagcca aagttggttg aagcgcctgc caccagttcc cgtgctgccc
      181 gacgggcaat caggcgacgc accggatgac taaccagttc aatagcgtcg ttagaactta
      241 gacggcgatg ttgaccgcta atggtcaggt cgtaaccacc aagataggtt tgcggttgtt
      301 cgcggtgctc aacgatgcca tcaaccagaa tccccggtaa tttgacccga cgtggttcaa
      361 tcgcgccagc ttccagcaca tcgcgtacct gtacgaacac tttgccgccg ctgttgtgtg
      421 ctgccagcgc catggaatag ctatccatat caatcgcttc ttcttcaagg ctgacgttgc
      481 ctcgaggatc ggcataagtg ccacgcaaaa tcgcgtaatc cactttgaaa gggcgataac
      541 gaagtttggt ttcaccatcg atttcaatca gttctaccag gtcggtggta gtgcattcat
      601 tcgacttacc gccgccattg cgtggatcaa caaacgatcc cagcccaacg tgggtaaaaa
      661 gccccggacg tccggcaccg atctcccgta gcaacgcctg aatcacgcca ccagggaaac
      721 agtaggcttc aatagcattg cttttcacca gttcctgcat cttgggcgac caggtaaaat
      781 gtccggcaat aatgcgtttg agcatttcgg cgtgagcaaa acggttagtg cctttgctgt
      841 cgcgatcgcc aatacccagc gagtggatta atgtcagatc gcgcgggtgt ccggtttgca
      901 ggaaacgcgc ttcaatagca gccagaatat ggtcggcttc caccataccg ccgccgttac
      961 cgctaatagc aatagtcgca ccgtcctgaa tttgcgccgc cagtgcctcg gcggttgtta
     1021 tcttacgcat acaatttcct tattgattat ggaagctcac gctacttttt tcggcaaagg
     1081 cgcgcgtcgc ctgctggcaa tcctgagtgg cggacattaa tccggccagt agctggtgcg
     1141 ggtaatcggc atgtttattt tccagtgcca gaatcagttg cttaatatgt gcaagcgcca
     1201 ccggaccaca tttttccagc tgcgctaact gattcgccat ccagctttcc acctgttctt
     1261 tttctaccac ttcatcaatt aaattcgccg cctgagcttc ctgcgccgtc aggcgtttac
     1321 ccagcatcag catctggcga ccgaccaccg gaccgacctg attaagcacg cgttcaatgc
     1381 ccatccagcc aggcaccatg ccgagcatta cttccggatt agagaatttc gcgctggggc
     1441 gagcaatacg aatatcgcag cacaaggcaa gttcaatacc accgccgatg gtatgaccgt
     1501 tgagattcgc aaccgttaat tgaggtaaat tgcgcaggcg attaaaaacg tcattgccgc
     1561 gtttaatcca ttttctgccc atatctaatg gcgaataggc tgaccacgat ttaatatcgc
     1621 cgcctgcaca aaagaattta tcgccggttg ccgttaattc gactaatcgt aatgtggtgt
     1681 cgttttcaat ttcatttaaa taatggtcga gagattcgat catttcttca ttaatagcat
     1741 gacatttatc ttcacggttc aatgtcaggc ggcaagaagc cgccgctctg ctgaacagaa
     1801 cgggttggtc actcatgatt attccttagc aataatgcgc gatgcgatcg acgctaatat
     1861 ccagtgtgct gtcaatatct tgctgccacc aatacggtga gaatatttcc agttcaatag
     1921 cgccattaaa tccggcgttt tcaaccagcc gacgaattga cggaatattg atgacaccat
     1981 ctcccggcat tccacggtca ttgaccagat cggtggtcgg cactaaccag tcggaaacat
     2041 gaaacgcgag gatgcgtttt ccggcacgca ggatctgact gacgagatcc ggatcccacc
     2101 agacatggta gacatctacc gccacgccga ggccaaattc gccatcggga tcgagttcat
     2161 cacaccagtc cagcgcctga cgtaacgtgc acagacaaga acgatcggca gcagtcatcg
     2221 ggtgcagtgg ctccagtgca attggcacac cgacatcttt cgaatgtggc agcaactggc
     2281 gaatcccttg tttcacctgt tcccgcgctt cgtacagatc ttttgttccc attggcagac
     2341 cgccaaccac ctgcatataa caggcggcgt tcagtacagc ggcgtcgtcc agcgcccggc
     2401 ggttatcgtc aatcgccagt ttgcgctccg ccagcgtcgg tgcggtgtag taggtgctac
     2461 ggcacaaacc ggaaacgttt atgttactgg cagcaagctg gcgggcgatt tgctggagat
     2521 cttcaccctg caactccctg cgccaggggg caatggcgcc aataccgcgt gcggcgcagg
     2581 catcaataat ggcgggcagc ggcgttttat agcccagtgt cgcggtattg atactgaata
     2641 aatccgggcg ttgtaacaga tctctcatga ttcagcctca gaagcgcggc atacttaagc
     2701 cgccgtcgat ggcgaccgcc tgaccacagg tgtaaatcag tttaccttcc gccatcgcgc
     2761 ggacagtgga agcaatatca gccggataac cccaacgtcc ccacggcacc agacctttgg
     2821 caatcagttc gtcgtaatag gccgttgccg gaatagtcat atcggttttg atgagaccag
     2881 gacgcacttc ataaacgcca atttgttcat tacaaaggcg agcagcaaac agacgagcag
     2941 cggcagaaac ggcggttttg gcaatggtgt attcgccgcg attcatcgcc agcatgatgg
     3001 cgttgattga actgacaaaa ataatcgagc ggtgcggcag ctccgcttcc ggtttcggct
     3061 gtgcgagcag acgtttactg aatgcctgag cgaggaagaa cggcgcgcgg gtattgatgg
     3121 caatgttttg gtcgaaactg tctggctcga gatcgaggag gtcaccgcgc tttttcactg
     3181 aaatccctgc gttgttgagc agacagtcca gacgccccca ctggttttgc gccgcgtcga
     3241 gcatctcttc atgcagggag agatcgccaa catcggcagg gaagcaaatg acctccacgc
     3301 cttctgccat acattcttgt tgtgtgatat gcagtttttc tacgctgtcg gcatcagggc
     3361 gatcgttaat cagcaggtta aacccgctac gagcaagttc cagcgcacaa ccctttccaa
     3421 taccacgtgc agcaccagtg atgacagcaa caggacgagt ggatttcata caatgaccct
     3481 cttttgaata ttgtttcctt gagtgatgat tagcttgcga ctggaaggta aaaagatcaa
     3541 ccatctagtt ggttgatatg cgagagtgat cacaggtaat ttgataactc aatgaaatta
     3601 cgacttttat cctttttgct gtcgctgaga tcaaatccgg ttatgatagc catcacgaag
     3661 aggaagtggg gaagtggaat gtatcttgat accagtcacg cacaaaacct gaaggaaaaa
     3721 ttgctgctat gcgcggttaa tgaatttgcc gaatatggtt acgaaggggc cagagtagat
     3781 aatattgtta aggcagcagg gtgcagtaaa cagacggttt atcaccactt tggtaataaa
     3841 gaaaatttgt ttattgaagt actggaatat acctggaacg atattcgcga aaaagagaga
     3901 gagcttgaca tttcagggct ttcgccggtt attgcgatcg agaaaataat tgattttact
     3961 tgggattatt acatcaataa cccgtggttt ttaaaaattg tccatagtga aaaccaaagt
     4021 aagggtgtgc attataaaaa atcaaaacgc ctcgctgaaa ttaaccatgc acatttgcag
     4081 tcaatggctt cattattaga agaagggcag cggcagaaaa cctttaagaa atatattgat
     4141 cccttacaaa tcaacattaa tatcgccgca ctgggtgcat attatttaat aaaccagtac
     4201 acactgggga tggtttatca cattagcatg gtttcacctg aggctttgga agccagacgc
     4261 agggtgatca aagagaccat cctgagctgg cttttggtga accctgcccg tgggtaatca
     4321 gcgtgcgcgg atgaaattac catcaggttg gcgggtgaac aggacttgct gattatttcc
     4381 cgtatcaatg gttaagccgg taaccacccc attcgcattt tgacggattt taacaatctg
     4441 accattttgc aggttactga gtggtttatc cgcgccctcg acctgggcca tagcgtacac
     4501 gtctgtaggc ggcaaattat ggtcgcggaa cagctgggca agggtcttgc ctggttctac
     4561 gcgatatgtc cgccactgtt gatcgatacc actttccggt tggaaagatt gtgtctctgc
     4621 aacaggtggc tcttcaggtt gaccttcctg aataggttcc ggcgcgaccg gggcgacctg
     4681 atccggatcg ttttgcggcg tcactaaatg tgcctgtagc tgtgcttcac ctgggggggg
     4741 tgattgcata tctaactgcg cttcgcgggt gacaacaggc atctggcgtg tttcatctcc
     4801 aggcagaaga ataccgataa tcagcactat tgccgcaata atgactcccc ggcgatgcat
     4861 cggcggcagc gggtccatga agcgaaaatt gtccggcgcg tgccagactt tctccagggt
     4921 tggttttagt tcaaagcgcc cgggcatggt ttcctcctgc tccgcgtctt gttcctcaat
     4981 catagcctat gaataagcta acgctatgat gtccgtggta aacccgcctt tattatattc
     5041 atacgggata ttgctattgt ttctttttcc ccgggatttg tcatcattcc cgcgacaaag
     5101 ttttacccaa agaagtgtgg ctgatatgct gcccgctact ttatacccta agaaaggaaa
     5161 tacgatgacc accccaactt ttgacaccat cgaagcgcaa gcaagctacg gcattggttt
     5221 gcaggtaggg caacaactga gtgaatctgg cctggaaggg ctgctgccag aagcactggt
     5281 tgcaggcatt gctgatgcgc tggaaggcaa acatccggct gttccggttg atgtggtgca
     5341 ccgcgcgctg cgtgaaatcc acgaacgcgc cgatgccgtt cgtcgtcagc gtttccaggc
     5401 gatggctgct gaaggtgtga aatacctgga agaaaacgcc aaaaaagaag gtgtaaatag
     5461 caccgaatct ggcctgcaat tccgcgtgat caaccagggt gaaggcgcaa ttccggcacg
     5521 taccgaccgc gttcgtgttc attacaccgg taaactgatc gacggcaccg tgtttgacag
     5581 ctccgttgct cgcggcgagc cagctgaatt cccggttaac ggcgtgatcc ctggctggat
     5641 tgaagcactg actctgatgc cggtaggttc taaatgggaa ctgactatcc cgcaggaact
     5701 ggcatatggc gagcgtggcg caggcgcgtc catccctccg ttcagcaccc tggtgtttga
     5761 agtcgaactg ctggaaatcc tctaagcagc gcattctgtt cccctcgaac cagaggggag
     5821 caggcattca gcaataaacc cttcagtttg ccaaacggcg ctattttatg ttgcaaagac
     5881 cccgtaagcg tgtatttttg tgagctgttt cgcgttatca ccgtgatatg acactcactt
     5941 taaacataaa attaacatta gatctaaatc ttagtattca tcccgcgtat tgttacctaa
     6001 tatcgatgag tcccgataca gattcgtcgt atcatagact gactaaaggc cgtagagcct
     6061 gaacaacaca gacaggtaca ggaagaaaaa aacatggtag atcaggtaaa agtcgttgcc
     6121 gatgatcagg ctccggctga acagtcgcta cggcgcaatc tcacaaaccg acatattcag
     6181 cttattgcca ttggcggtgc cattggtaca gggttgttta tggggtccgg caaaacgatt
     6241 agccttgccg ggccgtcgat cattttcgtt tatatgatca tcggttttat gctctttttc
     6301 gtgatgcggg caatggggga attgctgctt tcgaatctgg aatacaaatc ttttagtgac
     6361 ttcgcttccg atttactcgg gccgtgggca ggatatttca ccggctggac ttactggttc
     6421 tgctgggttg taaccggtat ggcagacgtg gttgccatta ccgcctatgc gcaattctgg
     6481 ttccctgggc tttctgactg ggttgcttcg ttatccgtga tcattctgtt actggttcta
     6541 aacctcgcca cggtaaaaat gttcggtgag atggagttct ggtttgcgat gatcaaaatc
     6601 gtcgccatcg tgtcgctgat tgttgtcggc ctggtcatgg tggcgatgca ctttcagtca
     6661 ccgaccggtg tggaagcatc atttgcacat ttgtggaatg acggcggctg gttcccgaaa
     6721 ggcttaagtg gcttctttgc tggattccag atagcggttt tcgctttcgt agggattgag
     6781 ctggtaggta ccaccgctgc ggaaaccaaa gatccagaga aatcactgcc acgcgcgatt
     6841 aactccattc cgatccgtat cattatgttc tacgtcttct cgctgattgt gattatgtcc
     6901 gtgacgccgt ggagttcggt agtcccggag aaaagcccgt tcgttgaact gtttgtgttg
     6961 gtaggtttgc ctgcggctgc cagcgtgatc aactttgtgg tgctgacctc tgcggcgtct
     7021 tccgctaaca gcggtgtctt ctctaccagc cgtatgctgt ttggtctggc ccaggaaggt
     7081 gtggcaccga aagcgttcgc taaactctct aagcgcgcag tacccgcgaa agggctgacc
     7141 ttctcttgta tctgtctgct cggcggcgtg gtgatgttgt atgtgaatcc cagcgtgatt
     7201 ggcgcgttca cgatgattac aaccgtttcc gcgattctgt ttatgtttgt ctggacgatt
     7261 atcctttgct cgtacctggt gtatcgcaaa cagcgtcctc atctgcatga gaagtcgatc
     7321 tacaagatgc cactcggcaa gctgatgtgc tgggtatgta tggcgttctt tgtgtttgtt
     7381 ctggtgttgt tgacactgga agatgacacc cgccaggcgc tgctggttac cccgctgtgg
     7441 tttatcgcgc tggggctggg ctggctgttt attggtaaaa aacgcatggc gaagtaatgc
     7501 cgcgtttata actctgaaag gcacctgcgg gtgcctttgt ctttatatat tcctaaaaaa
     7561 atctaccatc atagacagcc ctcaggcgcg agcttaccgt ggatctttgc cactcgttgc
     7621 agagttcatt atggttaagc gcagttggtt attaatcgcc gcattacctc cctctatttc
     7681 accttcctgg ggcgcggatt tttattaccg ccagcaggag aaaggcacgg tttatgttgt
     7741 cgaacagaag ggggaaaaag atgagatcct ctccgaatta ccagatatta atttttcccg
     7801 cctttggcgt attgccaatt tagccaataa acaagattcc cggttactgt ccgattttaa
     7861 tcccgataag ttcgattgcg atgatgaggg ggattgcgaa catgcctggc tcaccgatgg
     7921 acgctctgtt ctttggtctg gcaaagtcct gaaaaatccc cccggtaaac ctatagtcga
     7981 cgctgccagt tttcaggcat tcggcgcttt cgctgctgat aaacgcagta tctattttga
     8041 tggtcagcgt accgatgata atagcggtga taagcaggtg gatatgtcaa cgcttgaaga
     8101 gacggacatc tggaatttac tgcgtgataa aaatagtctt tggcataagg ggcactggtt
     8161 gggaagcgct gacggatttc aaatcctgcg gcatgattcc tccctgcaat ttgttgtgca
     8221 gacaaattcg caggtgattg ttaatggcaa gccactgccc gccgatcgca aaacttttca
     8281 gattaaacgt tggatgcctg gcgaacgctt agtttatcgc gataaaagcg gcgagcgtga
     8341 ctatgagctg gaggatacca gctatcgctg tgcacctttt aatattggtc tgaataacgt
     8401 gtcctggctc aaatatgaag ccactccagc gggcagtgag tgtatttatg aaacgctggc
     8461 gggagttgat ccggaatatt tttatctgtt tgttcggaat accggtttat ataagaacca
     8521 aatatataaa gtcacaatta acgccctggg cgaaggtgag ttggttaatc tcaagccaga
     8581 ggatctctcc gactcacttg aagcaggggg tagttgggga ttaactaaca cgtttatatc
     8641 aacagacggg cagctttaca ctcaacaagc gactggaatt gggaaagaac acgcccaaca
     8701 aggtgaatgg ctgcgttata acttaggcaa gggaggttgg ttatcggtga agcaaccccc
     8761 gagcgggctt aaacccttat ttaaataacc tattcacgct aacagcatca ggatgacgct
     8821 atgttaccaa ttaaacccgt ttgtctcgca ctggctttat tttcactcaa ttcaatagcc
     8881 tgctccggcg taccatctgc ctctttttat taccaaccgt gggggcaagg aacaaaatat
     8941 attgttgaag atagcgatgg ttacggactc tattttccgg atattcattt accgcaggtg
     9001 aggttcaaac aactgcgccg tgtatctgac ggcactgatg atgaatatga ttctcctgat
     9061 gatatcaata atgattttgt gcgggtggat ggtggcgctt atatcagaac caacggtggt
     9121 tggcgtttcg actggttgac tgatggccgc catattttat gggcagggaa aatagttcag
     9181 aacccgccgg gaacaccgaa agtcgatgcc gcaacattcc gcgcatgggg gcgttttgct
     9241 gccgataaag acagcctcta ttttgatggc gaacgcacgg atgataatca cggcgaaaag
     9301 caggtcgata tggattcgct gcaacaggtg gggggaagga tgaattcact tgattccgcg
     9361 gatgtgctga aagatcgtcg caatctttac tttcaggggc gctggttagg tagtgcgcaa
     9421 gggtattcta ttcttgacat taaaccctgg acacgtcgtc ccattattta ttcacctaat
     9481 agctgcgaaa gcaaaagtaa tccgggaccc tgggatacaa tctggcggac taaatcgcag
     9541 gtctttgtga atggagatgc catcaacgcc gatccggata cctttcatat tgtgcgctgg
     9601 atagccggtt cactgctaat ttatcgcgat aaagacgggg agaaacgtta tgcgttcggt
     9661 aaaaactgcc gaagtatatt tgacctacag cgggataaag taacctggat gacgcgtgat
     9721 gcttccaggc gtgctaatga ctgccgggtt gcgacaattc ctgatgttga tccggaatat
     9781 tttcgcccgc tgacggagaa tgtggcgcaa tataaagatt cgctctatct ggtgaaatat
     9841 atatctgcgg tcgaaaaaac gctgtcggtc atacatttac cagacccaca acaagaatta
     9901 cagaaaggtg ctaatgttgt agggaacaaa atttatttca tcgcgcgcga tgatgtgact
     9961 atttttgata ttaatgggca atggcagtgg tataaaagcc ccgatggcac accattaaat
    10021 tatttagcac atgacgatcg ctatacgtat tttattgatg aagacacggt tgagcatttt
    10081 gaactgaaag gccaatggac gtggtttaaa tacgccaatg gtcaattatc agataccttt
    10141 gctcatgatg accactatat ttattacgta ggcgatgggc tggtacgtga tgcgaagcgg
    10201 cgtaatgaga ctcgacaact cgatgcggcg catcttgata aaaacggcag tttgcttacc
    10261 gttgaaggta agtacaccag ttacaacaat gaattacttc cactgaatga ctgaataatc
    10321 aacgatcctg cccatgtcag gatcgttgat cgaacaaatc actcacccgc cagcgcgcgc
    10381 gggaatagta cattgttttc cagactgatg tgctccatca ggtcatcaat cagttcatta
    10441 atgccgttat acatcgcttt ccaggtggtg caggcttccg gcggcggtgt gacgttattg
    10501 gtggtgtgtt tgatcacttc cagcagttcg cccgcttcat cgtgctcgct ttccattacg
    10561 ctgattggcc ccattgcctg gctgcccatg ccttgtttga tcatcgggaa gagaatttgc
    10621 tcttctttca tcatatggct ggaaagctct tcatgcagca tagtcaggta ttttgtcagc
    10681 ccttttggca cacttggttt gtcggcgtga acgcgctcga ctttagtcgc ttgcaggatc
    10741 agctccggca gctgctcgcg atggcgatcg tgatagcgca caataatgtg gtcgatgatt
    10801 tccgccagcg gggcgctacg ccagtctttc tcaatcggtt gttcagcaag ctttgccagt
    10861 tcggcttcaa tgacgtcaac atccagttct ttacgtgccg ccgcgcgcgc cagcgtctgc
    10921 ttaccgccac agcagtaatc catatcatat ttacgaaaca gagccgaagc gcgaggaata
    10981 gagagcgcca gttcacctaa aggttggtcg cgataagcca tagctgatac ctcattctta
    11041 ataagataag atgcatttta aatgcatctt taaggcaaaa gctataaccc ttacgtagca
    11101 gaggcttatt taactcactg ccagcgtgtc aactttctga gacagattca gttaaaggtt
    11161 tttctggttt tgctctgacc gcgatgacca cgccaatcac taacagcgcg ataccgctca
    11221 gcgtcattag cggcggcatt tgctggcgga gtaaaaaggt gtacagcaaa cctgccagcg
    11281 tttcgaaaac aatcagcggc ccgagaatca ctgtcggtaa tcgctggctg gcgacgttcc
    11341 agcagagtgc gccaacccat gagcaaagca cggctatcgc aaccatcaga ctaataaaca
    11401 ccagcggacg ggggccaaag ggtaaggaga agtccggcgt ttgcatattc agccagtaac
    11461 aggcgacgag atagccgatg agagaaaccg gcagcgtgac cagcgcctgc gccgtcgccc
    11521 acatcattgg atgtttgtcg ggattttccc gcagccagcg ggcgttgcgc agggcatacc
    11581 atgcccagca gaccacggaa actaacgcta gcacgatgcc tgaggtataa cgtgcccagt
    11641 caaaatcggg gagtccgtgg tttaactcag caatattcac acacgccagg ccgatgccaa
    11701 tacaaatcag tgccggggcg agttttcccc acgcgagttt gccgtcgcgc tggctataaa
    11761 gcagattggc aaaaacggga atcaccaccg gcagggtgcc gataatcatc gtggaaacag
    11821 gcgcgccagt acgttgaatg gcactggcaa ggcagaagta atagatgagg ttgcccatca
    11881 tagtgagcat caaggcggta agccagtcgc gacgcgccaa ctgacgcaga cgcacgcgtc
    11941 ccagccaggc aatgggtagc gcaattaacc ctaacgccag ataacgcccc atcgactgca
    12001 acatcgccgg gtattccggc acgatcaacg gcccgacaaa aataagcccc cacatcaacc
    12061 ctgctaatag ggcgtacagc acgccgctaa tcattactgg catccattga tctgtcagaa
    12121 gaaagttcag gaagcagaca tgattgctcc cggcatcccg tcattatcag agaggatgcg
    12181 ggaggatgcc actttaatta actagttatt aacattaaaa agatggctta cgctttcggc
    12241 taacgttgtc gttgggcggc caatcaattt gctaagcgtt ttgctgttat caaacagacc
    12301 acctttcgat gcgccaacgt cagaatccgc cagcatatcc gccagtccgt caggcagtcc
    12361 gacgcttttc agtgccgcgg cgaaatcagc ttcactcaga ttttgatagg taacctgttt
    12421 gccgctctgt ttggtgagtt ccgccgctaa ctgtgtcagt gtccaggcgc tatcacctgc
    12481 cagttcataa accttgcctt cgtgacctgc ttcgctaatc acgcgtgccg cagctgccgc
    12541 ataatctgcc cgcgttgctg aggcgatttt gccatcgccc gccgcaccga taaatacgcc
    12601 gtgtttcagt gctgccgggg cgctggcgag gtagttttcg ctgtaccagc cgttgcgcag
    12661 cagggtgtaa acgatgccag aatcagccaa cattttctcc gtctcaatgt gctcatcggc
    12721 gaggccgagc ggagatttat ccgcgtgaag cagactggta taagcgataa atttcacacc
    12781 agccgccttt gcggcattaa taacattacg atgctgcggg gcacgttgac ccacttcgct
    12841 ggaagagatc agcagtagtt tttccactcc ctgaagcgca gatgtcagtg cggcttcatc
    12901 gccgtaatca gcctgacgca cggtaatgcc ttgtgctgtc agtgcctggg cttttgccgg
    12961 attacgaacg atagccacta tttggctggc aggaaccgtt ttcatcaagg attcaataac
    13021 atagtgacca agttggccgg ttgcgccggt aattgcgatc atgggaagcc tccatcggtt
    13081 tgtcttgtgt ttaaatacgc taccacctaa acttactttt agtaagtacg tacaaaaagg
    13141 taagtatgaa atgagtcagg ttagcctgtc gcaacaactg aaagagggta acctcttcgc
    13201 cgaacagtgc ccgtcgcgcg aggtgttgaa acacgtcacc agccgttggg gggtgttgat
    13261 tctggtggcg ctacgcgaag gtactcatcg ctttagcgac ctgcggcgca aaatgggcgg
    13321 ggtgagtgaa aagatgcttg cgcagtcgtt acaggcatta gaacaggatg gttttcttaa
    13381 ccgtatcgcg tatccggtgg tgccgccgca tgtggaatat agcctcacgc cgctgggcga
    13441 gcaggtgagc gaaaaggttg ctgcactggc ggactggatt gagttgaatt tgcccgaggt
    13501 gttagcggtg cgggatgaac gtgcggcata actgccggat gcgctgcgct tatccggact
    13561 aaggttcatg gcttgtaggg cggataagat gcgtcagcat cgcatccgcc aataaatgcc
    13621 ggatgcggtt gatgatttgc aggccgaaaa agaagtgtat tacttactca gatccacctg
    13681 ataaattgca aacccgatat catcggtcgc gactttattc atcggatact gccctttttc
    13741 tttaataaac gctgcggctt tatctgacgg agaggtttcg aaacggatat ccagtttctt
    13801 atcgccggct atcggtgcta aacgccagtt gttatctgcc gccgggtgaa tttcccccgc
    13861 acgtttcgac tcatcagcaa tccacgctgc cagcaccgag cggttctcat ccggtgaagc
    13921 aaaagcgata tggctgtcgc ccgtaccggc aaatttgccg ccgtaagcgc gatagttatt
    13981 ggtggcaacg aggaacatgg cgttcggatc aatcggcttg ccgttaaagg tcaggttctt
    14041 aatcctttcc gcattggcat taatcatctg gcactcgccg tcataacggg cgggctgggt
    14101 aacatcaatc tgataattca caccatcaat cacatcaaag ttataagtgc ggaaaccatc
    14161 ccagttaatg agtgactgtg gtttcgtgct gttaggatca atctggttaa actgtccggc
    14221 agagcattcc agccactctt tcacctcttt accgctggct ttcaccacaa tcagcgtatt
    14281 ggggtagaga taaagatcgg cggcattacg gaaagtcagc tgaccttttt ccacctccac
    14341 atagcttgcc gggtcatttt tgcgaccacc aactttaaac ggtgcggcag ctgaaagcac
    14401 tggcagtttt gccagatccg gatcgccctg aatgtaatgc tcgacatacg ctttttgcgc
    14461 gttgttcacc acctgcacgg tcggatcgtc ctgcaccagt gccagatagc tatacatatt
    14521 gtcggcagat ttaccgattg gcttgctgac gaactggcgt gtggcatcgt gatcggcttt
    14581 gagtgtttct accagcttgc tgtcttccgc cgcgagggat tttttattag cgatgtcgta
    14641 aatcggtcgt gcttccgctt tcgcctgtgt tacctgccat ttaccgctgt cattactgag
    14701 ttgtaagtcg accacaccaa gatgatcgcc ccacatgcct ggcattaccg ccggaacacc
    14761 attcagcgtg cctttcgcga tatcagctcc ttcgatatca gcaaaatctt tacccgggaa
    14821 aacggcgtga gcatggccaa acataatggc gttaacgccc ggaatttcac tgaggtaata
    14881 aactgagttt tccgccatca ctttatacgg atcggcagac agcccggaat gcgccagaac
    14941 gacaacgaca tcggcacctt tctcgcgcat ttcaggcacg tatttgcgca cggtttcggt
    15001 aatatcattc accgtcactt tcccggataa attagcttta tcccagccca tgatttgcgg
    15061 cggtacgacg ccaatatagc caatcttcag cgtctgtttt tttccgtctt tatcgaccac
    15121 ttcggtgtct ttaattaaat acggtgtaaa cattggctgt ttggttctgg cgtcaatgac
    15181 gttggcattt acataaggga atttcgctcc cgccagcgca tttttcaggt aatccagacc
    15241 gtagttaaac tcatggttgc caagcgtgcc gacggtatag tccagcgtat ttaatgcctt
    15301 atagaccggg tgaatatcac ctgcttttaa tcctttcgcc gacatgtaat ctgccagcgg
    15361 actcccctga atcaaatcgc cgttatcgac cagtacgctg tttttcactt cattgcgggc
    15421 atcattaatc aggcttgccg tacgtaccag tccgaatttt tccgtggcgg tgtctttgta
    15481 ataatcgaaa tccatcatgt tgctatgcag atcagtggtt tccatgatac gcagatcgac
    15541 cgtcgctgca ttcacactgg cggcaatcag cgtggccagg agcgttgcgc taaacttaat
    15601 catcagggac atccttttat catcgggaat acgaaagaaa agggagaata aacgtattac
    15661 ttatagaaca gtgaagaatg ccacaatttt acgctttgaa aatgatgaca ctatcacagt
    15721 tggcgcattc attaacgata gggtataagt aaaacaataa gttaacaccg ctcacagaga
    15781 cgaggtggag aaatgttaga tcaagtatgc cagcttgcac ggaatgcagg cgatgccatt
    15841 atgcaggtct acgacgggac gaaaccgatg gacgtcgtca gcaaagcgga caattctccg
    15901 gtaacggcag cggatattgc cgcacacacc gttatcatgg acggtttacg tacgctgaca
    15961 ccggatattc cggtcctttc tgaagaagat cctcccggtt gggaagtccg tcagcactgg
    16021 cagcgttact ggctggtaga cccgctggat ggtactaaag agtttattaa acgtaatggc
    16081 gaattcaccg ttaacattgc gctcatcgac cacggcaaac cgattttagg tgtggtgtat
    16141 gcgccggtaa tgaacgtaat gtacagcgcg gcagaaggca aagcctggaa agaagagtgc
    16201 ggtgtgcgca agcagattca ggtccgcgat gcgcgcccgc cgctggtggt gatcagccgt
    16261 tcccatgcag atgcggagct gaaagagtat ctgcaacagc ttggcgaaca tcagaccacg
    16321 tccatcggct cttcgctgaa attctgcctg gtggcggaag gacaggcgca gctgtacccg
    16381 cgtttcgggc caacgaatat ttgggatacc gccgctggtc atgctgtggc tgcggctgcc
    16441 ggagcgcacg ttcacgactg gcagggtaaa ccgctggatt acactccgcg cgagtcgttc
    16501 ctgaatccgg ggttcagagt gtctatttat taattaattt atgcagcagg tccatcactt
    16561 gctgcacctc ttcctgagta agcgccccgt ctttggccca ttgcacgcga ccgtctttat
    16621 ccagcaccac tacagcggaa cttttttcat ccagctgcca ggcaccgcgt gcgacgccat
    16681 tgctatcaac aataaactgc gaccagggat aaagcttttt attactctcc agactgctgc
    16741 gcacaaacat gccggaaccc ggaattgcgt cgtcggtgtt aacaatggtg gtggtctggt
    16801 aacgatcgtg cggtaacttt gctgatttaa tcgcttcaat cagcgtcgcg tttttctctt
    16861 ttgcagaggt gcgaccagca atatgttgca gtactcgcac ttttcccact aactgcgcgc
    16921 tgttccaggt tttgtagcta aactgatctt tatcaagcac caactcgcca cgatcggtga
    16981 tgccaatcgg cggcactcgc tgaccggttt cgaactgatg tgcggaagcc atcatcggca
    17041 acagcaggca ggtgagtgcc agaatcttgc gtagggtcat ggtgtttcct tcttatgata
    17101 tgcaggtgat ccgaccactt gggtctgagt tttaatcata tgtgctattt atcgaatttc
    17161 ccgcaagtgt gatgccagtt tgcggtcaag cgcacaaatc atatgaaaaa tgaatgctta
    17221 tactgaagac cgcgcttcgg taaaaagata attctgaata attgtaacct ttaggtaaaa
    17281 aaagttatac gcggtggaaa cattgcccgg atagtctata gtcactaggc attaaaattt
    17341 gcgcctcata atcgttgggc cgattgtggc accgcacagg cgtaatactc agcaggagat
    17401 aacaatgaaa attttccaac ggtacaaccc acttcaggtg gcgaagtacg taaagatcct
    17461 gttccgtgga cggttataca tcaaggacgt tggcgctttt gaatttgata agggtaagat
    17521 tcttatccca aaagtgaagg ataagctgca tttgtcagtg atgtccgaag ttaaccgtca
    17581 ggttatgcgt ctgcaaacag agatggctta accaaagtgc tatgcagtaa taagacggct
    17641 cctgattcag gagccgttga tgtttctggg ggttacgcga cgctttcttc tttatcttta
    17701 gcgtcaggca gttttggcga aagggcggtg gccttgctgt cgatccgggt caccagcagt
    17761 tggtcgatac ggtagttatc gatatccacc acttcaaatt tgtagccggc gaatttcacc
    17821 gaatcggtgc gtttcgggat cttacgcagc ataaacatca taaagccgcc gatggtttcg
    17881 tagttgcccg actgcgggaa ctcgtcaata tccagcacgc gcatgacgtc gtcaatcggg
    17941 gtgccgccgt caatcagcca tgagttctca tcacgggcga caatctgctc ttccagcccc
    18001 tgaccgacca gatcgcccat cagcgtggtc atcacgtcgt tgagggtgat gatccccact
    18061 accagcgcgt actcgttcat gatcaccgcg aagtcttcac ctgcggtttt aaaactttcc
    18121 aacgcttctg aaagggttaa cgtatccggc acaatcagcg tgttgcgaat ttgtacgccg
    18181 ctgttcagtg ccaggctttg gttagccagc acgcggttca gcaggtcttt agaatcgaca
    18241 tatccgatga tgtggtcaat atcttcatta cagacgagaa acttagagtg cggatgttcc
    18301 gccaccttat tcttcagact ttgctcatct tcgtggagat caaaccaaat cacgttttca
    18361 cgcggtgtca ttgaagacgg aacggtacgg gattccagct caaagacgtt ttcaatcagc
    18421 tcgtgttcct gtttacgtaa cacgcctgcc agcgcaccgg cttccactac cgcgtagatg
    18481 tcatcagaag tgatgtcatc tttacgtacc attggcagtt tgaaaatacg gaagattatg
    18541 ttcgccagtc cgttgaagaa ccacaccagc ggggtgcaaa cgtacaggca gaagcgcatc
    18601 gggttgatga tacgcaaagc cacagcttct ggcgcaatca taccgatgcg tttcggggtt
    18661 aaatccgcaa acaggataaa catgccagtc actaacgaga aagagagaat aaagctcagt
    18721 tgctcagaga gttctgccga catatagcgg gagaacaggc tgtgaaaagc tggagaaaat
    18781 gccgcatcac cgacgatacc gccgagaatc gccactgcgt tcagaccgat ttggaccacg
    18841 gtaaagaaca tgccgggatt ttcctgcata ttcagaacgc gttgggcatt tatattgcct
    18901 tcatcagcca gcagtttaag tttgattttg cgtgaggcgg caagcgagat ctcggacatc
    18961 gagaagaacg cacttacagc gatcaaacag agtatgacta aaatactgtt taacatatct
    19021 tatccgacct ttcaggccag atcctcggaa ggggaagttg attaatttgt gtgaaataca
    19081 cattgaaagc cgattcgaag aaagtgaacc ggcattttca gtggctagta tagcgtaagg
    19141 tactgtaaag ccgccagagg gttaaaattc agacagctga aaaatgaaaa actggctgat
    19201 gcgctatgcc gaacaggcct acgcagtccc cacaatttat tgattttgga cgattttgta
    19261 ggcctggtaa ggcgtttacg ccgcatccgg catagacaaa tagcacttta tcaacaatct
    19321 acatttgtac ccgtaacgct atgcttccgg cggcagacag acgccaattc cgcaaattcc
    19381 acagtaacca tacgggtttt tatgcagata ttgctggtgg tcatcttcgg cataataaaa
    19441 cggtgtggcg ttagcgattt ccgtggtgat gcgacgatcg tcatcggcgg caagcatcgc
    19501 cgcctgaaaa cgttccagac tggcgcgagc ggcggcatcc tgttctgggg tcagaggata
    19561 aatcgctgaa cgatactgcg taccgtggtc attgccctga cgcattccct gggcgggatc
    19621 gtgattctcc caaaatactt gtagcaactg ctcatagctg atgacggatg gatcgtaaac
    19681 aatgcgcacc gcttcggcat ggccagtatc gccggagcac acttcccgat aagtcggatt
    19741 tggcgtatag ccgccggtat agcctgcggc ggtgctgtaa acgccgggta actgccagaa
    19801 cagacgctcc acaccccaga aacaacccat cgcaaaaatg gcaatctcca ttccgtcagg
    19861 tacattggtc attgagtgac cgttgaccgc atgcagcgtg gctacgggca tcggggtgtt
    19921 acgtccaggc agggcatcgg cgggggaaac cagatgcttt ttatcaaata aactcatggt
    19981 gtcgctctcc cgaaaatcgg tcattggggt taaggttgta acaagaggcg tatttgcaca
    20041 caataaccgc tgtgaatagg tctaaagtaa aacataagaa atatttgggg tttagtctgc
    20101 tttttaatcc atattactgg atttttgtta agccgtttaa cggcgttcca ggggcaggaa
    20161 aaaaggatat tcaggagaaa atgtgcgcta tatccgacag ttatgctgtg taagcttact
    20221 ctgcttaagc ggatctgccg tcgccgcgaa catccgtcta caggtcgagg ggttatcggg
    20281 acagctggaa aagaacgttc gtgcgcagct ttctacgatt gaaagtgatg aagtgacgcc
    20341 agaccgtcgc tttcgcgcac gcgtcgatga tgccatccgc gaaggtctga aagcgctggg
    20401 ttattaccag ccgaccattg aatttgatct ccgtccaccg ccaaagaaag ggcgtcaggt
    20461 attgatcgcc aaagtcacgc caggcgtgcc ggtgttaatt ggcggcaccg atgtggtatt
    20521 gcgcggcggc gcgcggaccg ataaagacta tttgaaattg ctcgatactc gcccggctat
    20581 tggtacggta ctgaaccagg gcgattatga aaatttcaaa aagtccttaa ccagcattgc
    20641 gttgcgtaaa ggttatttcg atagcgaatt taccaaagcg cagctgggca ttgcgctcgg
    20701 cctgcataaa gccttctggg atattgatta taacagtggc gaacgttacc gctttgggca
    20761 tgtgaccttt gaaggatcac aaatccgcga tgaatacctg caaaatctgg tgccgtttaa
    20821 agagggcgat gagtacgaat cgaaagatct ggcagagctg aaccgtcgac tttctgctac
    20881 cggctggttt aactcggtcg tagtggctcc acaatttgat aaatcgcgcg aaacgaaagt
    20941 attaccattg acgggcgtgg tttcgccgcg aactgaaaac accatcgaaa ccggggtcgg
    21001 ttactctacg gacgtgggac cgcgcgtgaa agcgacgtgg aaaaagccgt ggatgaactc
    21061 ttatggtcac agtctgacca ccagtaccag tatttccgcg ccggaacaga tcctcgactt
    21121 cagctataaa atgccgctgt tgaagaatcc actggaacaa tattatctgg tgcagggcgg
    21181 ttttaagcgc actgacctga acgataccga gtctgactcc actacgctgg tggcttctcg
    21241 ctactgggat ctctccagcg gctggcagcg tgccattaac ctgcgctgga gcctcgatca
    21301 ctttacccag ggtgaaatta ccaacaccac gatgctgttt tatcctgggg tgatgattag
    21361 ccgcacgcgt tctcgcggtg gcctgatgcc aacctggggc gactcgcaac gctactctat
    21421 cgactactcc aacacggcct ggggctcaga tgtcgatttc tccgttttcc aggcgcagaa
    21481 cgtctggatc cgcacactgt acgatcgcca tcgttttgtg acacgcggca cgctgggctg
    21541 gattgaaacg ggtgatttcg acaaagtacc gccggatctg cgtttcttcg ccgggggcga
    21601 tcgcagtatt cgcggctaca aatacaaatc aatcgctccg aaatacgcca acggtgacct
    21661 gaaaggggcc tcgaagttga taaccggatc gctggagtac cagtacaacg tgaccggaaa
    21721 atggtggggc gcggtgtttg tcgatagcgg cgaagcggta agcgatattc gccgcagcga
    21781 ctttaaaacc ggtaccgggg tcggcgtgcg ctgggaatcg ccggtcgggc caatcaagct
    21841 cgattttgcc gtaccggtcg cggataaaga cgagcacggg ttacagtttt acatcggtct
    21901 ggggccagaa ttatgagttt atggaaaaaa atcagcctcg gcgtggttat cgttatctta
    21961 ctgttgctgg gatcggtggc gtttctggtg ggcaccacta gcggcctgca tctggtattt
    22021 aaagcggcgg atcgctgggt gccaggactg gatattggca aggtcaccgg cggctggcgc
    22081 gatctcacct tgtctgacgt tcgttatgag cagccaggcg tggcggtaaa agcgggcaat
    22141 ctgcatctgg cggtcgggct tgagtgcctg tggaacagta gcgtttgtat taatgacctg
    22201 gcgctgaaag acattcaggt caacatcgac agtaaaaaaa tgcctccttc tgaacaggtt
    22261 gaagaagagg aagatagcgg tccgctggat ctctccacgc cgtatcccat taccctgaca
    22321 cgggtggcgc tggacaacgt caacatcaag attgatgaca ccacggtatc ggtgatggac
    22381 ttcacctccg gcctgaactg gcaggagaaa actctgaccc tgaaaccgac gtcgctgaaa
    22441 ggcttgctga ttgccttgcc gaaggtggcg gacgtggcgc aggaagaagt ggtcgaaccg
    22501 aagattgaaa atccgcagcc tgatgaaaaa ccgctcggcg aaacgctgaa agatctcttt
    22561 tctcgcccgg tattgccgga aatgaccgac gtgcatttgc cgcttaacct gaacattgaa
    22621 gagtttaagg gcgagcagct gcgcgtgacg ggtgacacgg acatcaccgt gagcaccatg
    22681 ctgctgaaag tgagcagcat cgacggtaat actaaactgg acgccctgga tatcgattcc
    22741 agtcaaggga tcgtcaacgc cagcggcacg gcgcagctgt cagacaactg gccggtggat
    22801 atcaccctca acagcacact gaacgtggag ccgttgaaag gtgaaaaggt gaagctgaaa
    22861 gtgggcggcg cgctgcgcga acagctggag attggcgtaa acctttccgg tccggtggat
    22921 atggatttac gcgcccagac gcgactggcg gaagccggat tgccgctcaa cgtggaagtg
    22981 aacagcaaac agctttactg gccgttcact ggtgagaagc agtatcaggc ggatgatctg
    23041 aaactgaaac tcaccggtaa aatgaccgat tacacgctct ctatgcgaac ggcagtgaag
    23101 ggacaggaga tcccgccagc caccattacc cttgatgcca aaggtaatga acagcaggtc
    23161 aatctcgaca aacttaccgt cgcggcgctg gaagggaaaa ctgaactcaa ggcgttgctc
    23221 gactggcagc aggcaattag ctggcgcggt gagctaacgc ttaacggcat taacaccgcc
    23281 aaagagttcc cggagtggcc gtcgaaactc aatggcttga ttaaaacccg cggtagcctg
    23341 tacggcggta cctggcagat ggaggtgcca gaactgaagc tgaccggtaa cgtcaaacag
    23401 aacaaagtga acgttgacgg cacgctgaaa ggcaacagtt atatgcagtg gaagatccca
    23461 gggctgcatc tggaactggg gccaaacagt gccgaagtga aaggcgagct gggggtaaaa
    23521 gatctcaatc ttgatgccac catcaacgcg ccggggctgg ataacgcgct gccggggctt
    23581 ggcggtacag cgaaagggct ggtgaaagtt cgcggcacgg tggaagcgcc acaactactg
    23641 gcagatatca ccgcgcgcgg cctgcgctgg caggaacttt ccgtggcgca ggttcgcgtg
    23701 gaaggcgata tcaaatccac cgatcagatt gcgggtaaac ttgacgtacg cgttgagcaa
    23761 atctcgcagc ccgatgtgag tatcaacctc gtcaccctga atgccaaagg cagcgaaaaa
    23821 cagcacgagt tacagttgcg gattcagggc gagccggttt ccgggcagct taatctggca
    23881 ggaagttttg atcgcaaaga agaacgctgg aagggaactc ttagcaatac ccgcttccag
    23941 acgccggtcg gcccgtggtc gctgacccgc gatattgcgc tggattaccg caataaggag
    24001 caaaaaatca gcatcgggcc acactgttgg cttaacccga atgcggaatt gtgcgtgccg
    24061 caaactatcg atgcgggggc cgaagggcgt gcggtggtga atctcaaccg cttcgacctc
    24121 gccatgctga aaccgtttat gccagaaacc actcaggcca gcggtatctt cacgggtaaa
    24181 gcggacgttg cctgggacac cacgaaagag gggctgccgc agggcagtat caccctttcg
    24241 gggcgtaatg tgcaggtaac gcaaaccgtc aacgatgcgg cgctgcccgt ggcgtttcag
    24301 acgctgaatc tgacggcgga attgcgtaac aaccgtgccg aattgggctg gaccatccgc
    24361 ctgaccaata acggccagtt tgatggacag gtgcaggtga ccgatccgca aggccgccgt
    24421 aatcttggtg gcaacgtcaa tatccgtaac ttcaaccttg cgatgataaa ccctatcttt
    24481 acccgtgggg aaaaagctgc ggggatggtg agtgccaact tacgtctggg tggtgatgtg
    24541 caaagcccgc agttgtttgg gcagctacag gttacgggtg tggatatcga cggcaacttt
    24601 atgccgtttg atatgcagcc gagccagctt gcggtcaact ttagcggtat gcgctcgacg
    24661 cttgccggta cagtacggac ccagcagggt gagatctacc tgaacggcga cgccgactgg
    24721 agccagattg aaaactggcg ggcgcgggta acagcgaagg gcagtaaagt gcgtatcacc
    24781 gtgccgccga tggtacgaat ggatgtatca ccagatgttg tattcgaggc tacaccaaac
    24841 ctgtttaccc tcgatggtcg cgtggatgtc ccgtgggcgc gcatcgtggt gcatgagctg
    24901 ccggaaagcg cggtgggcgt ctccagcgat gtggtgatgc ttaacgataa cctgcaaccg
    24961 gaagaggcaa aaacggcgtc gattccgatt aacagcaacc tgattgtcca cgtcggcaac
    25021 aatgtgcgca ttgacgcctt tggcctgaaa gcgcggctga cgggcgatct taacgtcgta
    25081 caggataaac aagggctggg cctgaacggg cagatcaaca tccctgaagg gcgcttccat
    25141 gcctatggtc aggatctgat tgtgcgtaag ggtgagttac tgttctctgg tccgccggat
    25201 caaccgtatc ttaatatcga agctattcgt aacccggatg ctacagaaga cgacgtaatc
    25261 gccggagttc gcgtcactgg tctggcggac gaaccgaaag cggagatctt ctctgacccg
    25321 gcgatgtcgc aacaggctgc cttgtcttat ttgctacgtg gacaagggtt ggagagcgat
    25381 cagagcgaca gtgcggcgat gacctcgatg ctgattggtc tgggggttgc acaaagtggt
    25441 cagattgtgg gtaaaatcgg cgagacgttt ggcgtaagca atttagcgct cgacacccag
    25501 ggagtaggcg actcctccca ggtggtggtc agcggctatg tattgccagg tctgcaagtg
    25561 aaatacggcg tgggtatatt tgactctata gcaacactca cgttacgtta tcgcctgatg
    25621 cctaagctat atctggaagc cgtgtctggt gtagaccagg cactggattt gctctatcag
    25681 ttcgagtttt agcaatgcga atatttgtct acggcagttt acgccacaaa caaggcaaca
    25741 gtcactggat gaccaatgcc cagttactgg gcgatttcag tatcgataac taccagttgt
    25801 atagcctggg ccactatcca ggcgcagttc cggggaacgg aacagtacac ggtgaagttt
    25861 atcgtattga caacgccacg ctggccgaac ttgatgcctt gcgcaccagg ggcggtgaat
    25921 acgcgcgcca gttgattcag acgccgtacg ggagtgcatg gatgtacgtt tatcaacgac
    25981 ccgtcgatgg attaaagcta attgaaagcg gcgactggtt agacagggat aagtaaccat
    26041 acgcatacgc caccttcggg tggcgttgtt ttttgcgaga cgactcgcat tctgttttgt
    26101 aattccctca ccttttgctt ttctctccga gccgctttcc atatctgtta acgcataaaa
    26161 aactctgctg gcgttcacaa atgcgcaggg gtaaaacgtt tcctgtagca ccgtgagtta
    26221 tactttgtat aacttaagga ggtgcagatg cgtattacca taaaaagatg ggggaacagt
    26281 tcaggtatgg tcattcctaa tgttgtgatg aaagaactta acttacggcc ggggcagagc
    26341 gtggaggcgc aagtgagcaa caatcaactg attctgacac ccatctccag gcgctactcg
    26401 cttgatgaac tgctggcaca gtgtgacatg aacgccacgg aacttagcga gcaggatgtc
    26461 tggggtaaat ccacccctgc gggtgacgaa atatggtaaa gaaaagtgaa tttgaacggg
    26521 gagacattgt gctggttggc tttgatccag caagcggcca tgaacagcaa ggtgctggtc
    26581 gacctgcgct tgtgctctcc gttcaagcct ttaatcaact gggaatgacg ctggtggccc
    26641 ccattacgca gggcggaaat tttgcccgtt atgccggatt tagcgttcct ttacattgcg
    26701 aagaaggcga tgtgcacggc gtggtgctgg tgaatcaggt gcggatgatg gatctacgcg
    26761 cccggctggc aaagcgtatt ggtctggctg cgggtgaggt ggtggaagag gcgttattac
    26821 gtttgcaggc ggtggtggaa taaggtgtgt ttatttatcg cgggcataaa aaacccctac
    26881 taaccgaagc ccggcgttca gggttattac gctagaagaa cttatttatt ctttgcgcgc
    26941 tcgaaggagg caacgatttc agctttagcg gcttctgcgt tttcccagcc ttcaactttc
    27001 acccacttgc ctttttcgag gtctttgtag tgctcgaaga agtgagcgat ctgtgctttc
    27061 agcagttcag gcagatcgtt aacgtcttta atgtgatcgt attctttgct cagcttgctg
    27121 tgcggaaccg caaccagttt cgcatcttca ccggcttcgt cggtcatttt cagaacgcca
    27181 acaggacggc aacggatcac agaacccggc tgcagcgggt acggagtcgg gaccagtacg
    27241 tcaaccgggt caccgtccag agacagggtg tggttgatgt aaccgtagtt gcacggatag
    27301 aacatcgcgg tggacatgaa gcggtcaacg aacagtgcgc cgctctcttt gtcgatttcg
    27361 tatttgatcg gatctgcgtt agccgggatc tcaataacaa cgtagatgtc ttccggcaga
    27421 tctttacccg caggaacgtt gagtaagctc atgtctgttt cctttaaaaa tatgtggcaa
    27481 acaagtgccg agtattatag ccaactcgcg ccgaatgtct tcgcttgttt tcttcttcat
    27541 tttccttttt agccagtttt aagatggttt aaagagcagg aaaatccata accgtagccg
    27601 catttttcat agtgagatga aagcgattac aaacttgtga ttaacgtttt atttactttt
    27661 ttgaagtgtg atgtaacgca atccgttaca taacgaattg tctatagttt tttcgcgaac
    27721 atcttttcac caataataat taccccgacg aggacaaccc tatgtggaaa cgcttacttg
    27781 tagtctctgc agtctcggca gccatgtcgt ctatggcgtt ggccgctcca ttaaccgttg
    27841 gattttcgca ggtcggatcg gaatccggct ggcgcgccgc agaaaccaat gtggcgaaaa
    27901 gtgaagccga aaagcgcgga attacgttga aaattgccga tggtcagcaa aagcaggaaa
    27961 accagattaa agcggtacgt tccttcgtcg cgcaaggggt ggatgcgatc tttatcgctc
    28021 cagtggtagc gaccggttgg gagccggtat taaaagaggc gaaagatgcc gaaatcccgg
    28081 tcttcttgct tgaccgttcc atcgatgtga aagacaaatc tctctatatg accactgtca
    28141 ccgccgacaa catcctcgaa ggcaagttga ttggtgactg gctggtaaaa gaagtgaatg
    28201 gcaaaccatg caacgtggtg gagttgcagg gcaccgttgg agccagcgtc gccattgacc
    28261 gtaagaaagg ctttgccgaa gccattaaga atgcgccaaa tatcaaaatt atccgctcgc
    28321 agtcaggtga cttcacccgc agcaaaggca aagaagtgat ggagagcttt atcaaagcgg
    28381 aaaacaacgg caaaaacatc tgcatggttt acgcccataa cgatgacatg gtaattggtg
    28441 caattcaggc aattaaagaa gcgggcctga aaccgggcaa agatatcctg acaggctcta
    28501 tcgacggcgt gccggacatc tacaaagcga tgattgatgg cgaagcgaac gccagcgttg
    28561 aactaacgcc gaatatggca ggccctgctt ttgacgcgct ggagaaatac aaaaaagacg
    28621 gcaccatgcc tgaaaagctg acgctgacca agtccaccct ttacctgcct gataccgcaa
    28681 aagaagagtt agagaagaag aaaaatatgg ggtattgagg ggcatcaatg cctaatgccg
    28741 atttgtgggc cgccggataa ggcattcacg ccgcatccgg cggttgtgcg agatgcctga
    28801 tgcgacgctg ttgtgtctta tctggcgcac gctttacaac gggggaaact atgaatacta
    28861 accaacacca ggaaatcctc cgcaccgaag gattaagtaa atttttcccc ggcgtcaaag
    28921 cgttagacaa cgttgatttc agcctgcgtc gcggcgaaat tatggcgctg ctcggtgaaa
    28981 acggggcggg aaaatcaacg ctaatcaaag ccttaaccgg tgtctaccac gccgatcgtg
    29041 gcaccatctg gctggaaggc cagactatct caccgaaaaa caccgcccac gcacaacaac
    29101 tcggcatcgg caccgtctat caggaagtca acctgctacc caatatgtcg gtcgctgata
    29161 atctattcat aggtcgcgaa cccaaacgtt tcggccttct acgccgtaaa gagatggaaa
    29221 agcgcgccac cgaactgatg gcatcttacg gtttctccct cgacgtgcgc gaaccgctca
    29281 accgcttttc agtcgcgatg cagcaaatcg tcgccatttg ccgggccatc gatctctccg
    29341 ccaaagtgct gatcctcgat gaacccaccg ccagcctcga cactcaggaa gtagagttac
    29401 tgtttggcct gatgcgtcag ttgcgcgatc gcggcgtcag cctgatcttc gttactcact
    29461 ttctcgatca ggtctatcag gtcagcgatc ggatcaccgt cttacgcaac ggcagtttcg
    29521 taggctgtcg ggaaacccgc gagctaccac agatcgaact ggtaaaaatg atgctggggc
    29581 gcgagctgga cacccacgcg ctacagcgtg ccggacgaac attgttgagc gacaaacccg
    29641 tcgccgcgtt caaaaattac ggcaaaaaag gaatgatcgc accgtttgat ctcgaagtgc
    29701 gccccggcga gatcgtcggt ctggcaggct tgctgggatc aggacgtacc gaaaccgccg
    29761 aagtgatctt cggtattaaa cctgctgaca gcggcacggc gttgatcaaa ggcaaaccgc
    29821 aaaccctgcg atcgccacat caggcttcgg tacttggcat tggcttttgc ccggaagaca
    29881 ggaaaaccga tggcatcatc gccgccgcct cggtgcggga aaatatcatt ctcgcgctac
    29941 aagcccagcg cggctggctg cgaccgatct cccgcaaaga acagcaagag attgccgaac
    30001 gctttatccg ccagcttggc attcgcaccc cttccactga acaaccgatt gaatttctct
    30061 ccggcggcaa tcagcaaaaa gtgctgctct ctcgctggct gctgactcgt ccgcaatttc
    30121 tgatcctcga cgagccaacg cgtggaattg acgttggtgc gcacgccgag atcatccgcc
    30181 tgattgaaac gctatgcgct gacggtctgg cgctgctggt gatctcctct gaactggaag
    30241 agctggtggg ctatgccgat cgggtgatta tcatgcgcga tcgcaaacag gtggcggaga
    30301 tcccgctggc agcgctttcc gttccggcga tcatgaacgc cattgcggcg taaggagaac
    30361 agtgtgatgc ctcaatctct cccggacact acgccgtcga aaaggcgctt tcgctggcca
    30421 acgggaatgc cgcagctggc ggcactattg ctggtgctgc tggtcgacag cctggtagcc
    30481 ccgcatttct ggcaagtggt gcttcaggac gggcgtttgt tcggtagccc catcgacatt
    30541 cttaaccgtg cggcacccgt tgcgctgttg gcgattggca tgacgctggt gatcgccacc
    30601 ggtggtatcg atctctccgt aggggcagtg atggctatcg ccggagcgac taccgctgcg
    30661 atgacggtcg cgggatttag cctgccgatt gttttgttaa gcgccctggg caccggcatc
    30721 ctggcgggat tgtggaacgg cattctggtg gcaatcctta aaattcagcc gtttgtcgcc
    30781 accctgatcc tgatggttgc cgggcgcggc gtggcgcagt tgatcaccgc cgggcagatc
    30841 gtcacgttta actcgccgga tctctcatgg ttcggcagcg gatcgctgtt gttcctgcca
    30901 acgcccgtca ttattgcggt gctgacgctt ctcctcttct ggctgttgac ccgcaaaacg
    30961 gcgctgggaa tgtttatcga agccgttggt atcaacattc gggcggcaaa aaatgccgga
    31021 gttaacacgc gaatcatcgt catgctcacc tacgtgttga gcgggctatg tgcggcgatt
    31081 gcgggcatta tcgtggcggc ggatattcgc ggtgccgatg ccaataacgc cgggttatgg
    31141 ctggagctgg acgccattct cgcggtggtg attggcggcg gatcgctgat gggcgggcgc
    31201 tttaacctgc tgctttcggt ggtgggggcg ctgattattc agggaatgaa caccggaatt
    31261 ttgctttctg gctttccgcc ggagatgaac caggtcgtaa aagcggtggt ggtgctttgt
    31321 gtgctgattg ttcagtcgca acgctttatc agtctgatta aaggggtacg taaccgtgat
    31381 aaaacgtaat ttgccactga tgatcaccat cggcgtcttt gtgttgggtt atatctactg
    31441 tctgacccag tttcccggtt ttgcttccac aagagtgatc tgcaatatcc tgactgataa
    31501 tgcctttctt gggataatcg ccgttggtat gacctttgtg atcctctccg gtgggatcga
    31561 tctctccgtc ggttcggtga tcgcctttac cggcgtgttt ctggcaaaag tgattggcga
    31621 tttcggcctc tcgccgttgc tggcgtttcc gctagtgctg gtgatgggct gtgctttcgg
    31681 cgcatttatg gggcttttga tcgacgccct gaagatcccg gcgtttatta ttactctggc
    31741 gggaatgttc tttttgcgtg gcgtcagcta tctcgtttcg gaagagtcga tcccgataaa
    31801 tcatcctgtt tatgacacgc tctcaagcct tgcatggaaa atccctggcg gcggtcgctt
    31861 aagtgcgatg gggctgctga tgctggcggt ggtggttatc ggtattttcc tcgcgcatcg
    31921 tacccgtttt ggtaatcagg tatacgccat tggcggcaac gcaacgtcgg cgaacctgat
    31981 ggggatttcc actcgcagca ccactattcg catttatatg ctctccaccg gactggcaac
    32041 gctggcgggg attgtcttct cgatttatac ccaggccgga tatgcgctgg cgggcgtagg
    32101 tgtggaactg gatgctatcg cctcagtggt aattggcggt acgcttttga gcggtggcgt
    32161 tggaacggta ttagggacgc tttttggcgt ggcgattcag ggactgattc agacttacat
    32221 aaactttgat ggcacgctga gttcctggtg gacgaaaatc gccatcggta ttttgttgtt
    32281 tatttttata gcattacagc gtggattaac ggtgctgtgg gagaatcgtc agagttcgcc
    32341 tgtgacaaga gtcaacattg cgcagcgata aaaacgcctc tccgtgtgga gaggcgcagg
    32401 agattacgcg tccgggaact cacggataaa gcgttcgaca tcttcaacca tatggtcgtt
    32461 gccgacaaag aacgaacggc gctggtgcag ggtttccggg atgatatcca gaatacgctc
    32521 tttgccatcg ctcgctttac cgcctgcttg ttccgccagg aatgccatcg ggttgcactc
    32581 atacagtaaa cgcagtttac cgtccgggtg gctggcggtg cttgggtaga gataaatacc
    32641 gcctttcagc aggttacggt ggaaatccgc gaccagtgaa ccgatataac gtgaggtata
    32701 cgggcgattg gtagacttat cttcttcctg gcagaattta atgtacttct tcaccccgtt
    32761 cggaaactta atgtagtttc cttcgttgat ggagtaggtt ttgcctttct ccgggaagcg
    32821 catccgttcc tggcacaggc agaaaacgcc cagcgaagga tcgtaagtaa aggcgtgaac
    32881 accgcatccg gtggtgtaaa ccagcatggt agaggagccg tataccacgt aacctgccgc
    32941 aacctgtttg ttaccaggct ggaggaaatc ttcttccgtt accggcgtgc caacaggtgt
    33001 aacgcggcgg tagatggaga aaatggtacc gacagagacg ttaacatcga tgttggacga
    33061 gccatccagg gggtccatca gcaccacgta ttttgcgtgt tcacagcctt caaagacgac
    33121 aatctcatct tcttcttcag aggcaatgcc cgcaacgata tcgcgtgctt tcagtgcggc
    33181 tttcagtttt tcattagcga acaagtcgag tttctgctga acctcgccct gcacattctc
    33241 agcaccgctg gcacccagga tatcaaccag tcctgctttg ttgatatcgc gatggataat
    33301 cttggcgccc agttttattg ccgacagcaa agcagtgagc tcaccggtag catgagaaaa
    33361 ctcgtgctgc ttttcgacaa taaattcacc taacgttttc ataaaacttt ccctgcaatg
    33421 tttatggagt aaagcgaccg caacaatctt aacaaataat ctcaatgttg cgctcaggtg
    33481 aatcgcgcca gcaaattgcg gattatcctg aaatgcgttt ctcacttgcc cgacatatgc
    33541 gtaaaatgag cggcagatta aaaaaggata gtgacgtatg cgcattcata ttttaggaat
    33601 ttgtggcacg tttatgggcg gtctggcgat gctggcgcgc cagttaggcc atgaagtaac
    33661 gggttcggac gccaatgtgt atccgccgat gagcacctta cttgagaagc aaggcattga
    33721 actgattcag ggttacgatg ccagccagct cgacccgcag ccggatctgg tgattattgg
    33781 caacgccatg acccgtggaa atccgtgtgt ggaagcggta ctggaaaaaa acatccctta
    33841 tatgtcaggt ccgcagtggc tgcacgattt tgtgctgcgt gaccgctggg tgctggccgt
    33901 tgccggtaca cacggcaaaa ccaccacggc gggaatggcg acctggattc tggaacagtg
    33961 tggttacaaa ccaggatttg tgatcggcgg tgtgccgggg aactttgagg tctcggcgtg
    34021 tctgggcgaa agcgatttct ttgttatcga agcggatgag tacgactgcg ccttcttcga
    34081 caaacgctct aaatttgtcc attactgccc gcgtacgctg atcctcaaca accttgagtt
    34141 cgatcacgcc gatatctttg acgacctgaa agcgatccag aaacagttcc accatctggt
    34201 gcgtatcgtt ccggggcagg gccgtattat ctggccggaa aacgacatca acctgaaaca
    34261 gaccatggcg atgggctgct ggagcgagca ggagctggtg ggcgagcagg ggcactggca
    34321 ggcgaaaaag ctgaccactg acgcttccga atgggaagtc ttgctggatg gcgaaaaagt
    34381 gggcgaagtg aaatggtcgc tggtgggcga acataatatg cacaacggcc tgatggcgat
    34441 tgcggcggcc cgtcatgttg gcgtagcgcc agcagatgct gctaacgcgc tgggttcgtt
    34501 tattaacgct cgtcgccgcc tggagttgcg tggtgaagcg aatggcgtca cggtatatga
    34561 cgattttgcc catcacccga cggcgattct ggcaacgctg gcggcgctgc gtggcaaagt
    34621 tggcggtacg gcgcgcatta ttgccgtgct ggagccgcgt tcgaatacca tgaaaatggg
    34681 gatctgcaaa gacgatctgg caccttcatt aggtcgtgcc gatgaagtct tcctgctgca
    34741 accggcgcat attccgtggc aggtggcaga agtggcagaa gcctgcgttc agcctgcaca
    34801 ctggagcggc gatgtggata cgctggcaga tatggtggtg aaaaccgctc agcctggcga
    34861 ccatattttg gtgatgagca acggcggttt tggtgggatc catcagaaac tgctggatgg
    34921 gctggcgaag aaagcggaag ccgcgcagta attcggcctc agcctgatat agtattgccg
    34981 gataaggcgt tcacgccgca tccggcaaac catactcaca tcaacaacga aaattaccct
    35041 tcgttttccg ctaactcacg cagatactgg aaaatctggc gcgcggattt cggcggctta
    35101 ttcccttctt tctctttctt cgcgttacgg atcagagtac gcagttgctg acgatcggca
    35161 tcgggccaca gattcaatac ttcagcgatg gcatcatcac cctggtcgat cagacgatcg
    35221 cgcaggtttt ccagtttatg gaacagcacc acctgctggt tgtggcggtt tttcagctta
    35281 tccagcgcct gacgaatagg ctctacgtcg cgctggcgca gcatcttgcc gatgagctgc
    35341 aactggcggc ggcgaccttc catcttaata cgctgggcta actcaatagc cgcacgcaga
    35401 tccgcatcta acgggatctt atccagcgcg tttttcccca gatcaacaat ttctgcgccc
    35461 aggcgtttta gctcctcggc gtcacgttta atttcacttt tactgaccca gataatttca
    35521 tcgtcttcgt cttcgatgtc atcaccggga acgtcgtcga gccagtcttc gggctgctta
    35581 gtcatctcag gctccttaaa aaaagaggct aatgttacca gttaagatgc gcactgaaaa
    35641 acggttctct gttagacttc agagaaactc tctacattat ggcacttgca atgaaagtaa
    35701 tctctcaagt tgaagcgcag cgcaagattc tggaagaagc agtttcgact gcgctggagt
    35761 tggcctcagg caaatcggac ggtgcagaag ttgccgtcag caagaccacc ggcattagcg
    35821 taagcacgcg ttatggtgaa gtggagaatg ttgaattcaa tagcgatggc gcgctgggga
    35881 tcactgttta tcaccagaac cgcaaaggga gcgcatcgtc taccgattta agcccgcagg
    35941 ccattgcccg caccgtacag gcggcgctgg atattgcccg ttatacctcg ccagatccct
    36001 gtgccggcgt ggcagacaaa gagctgctgg cctttgacgc accggatctc gacttgttcc
    36061 accctgcgga agtttccccg gatgaagcca ttgaactggc ggcccgcgcg gaacaggcgg
    36121 cattgcaggc ggacaaacgc atcaccaata ccgaaggcgg tagctttaac agccactacg
    36181 gtgtcaaagt ttttggcaac agccacggca tgttgcaggg ttactgctca acgcgtcatt
    36241 cgctctccag ttgtgtaatc gccgaagaaa atggcgatat ggagcgcgat tatgcctaca
    36301 ccattgggcg tgcgatgagc gatctacaaa cgccagagtg ggttggggcc gactgtgctc
    36361 gccgcacttt atcgcgtctg tcaccgcgta aactctccac catgaaagcg ccggtcattt
    36421 ttgccaatga agtggcaaca gggttgtttg gtcatttggt gggggcgata gcgggtggtg
    36481 cggtctaccg taaatcgacc ttcttgctgg attcgctggg taaacaaatt ctgccggact
    36541 ggctgactat tgaagaacat ccgcatctgc tgaaagggct ggcatctaca ccattcgaca
    36601 gcgaaggtgt gcgcaccgag cgccgcgata tcgtcaaaga tggcatcctg actcagtggc
    36661 tgctgaccag ctattcggcg cgaaaactgg ggctgaaaag caccggacat gcgggcggta
    36721 ttcacaactg gcggattgcc ggacaagggc taagcttcga acaaatgctc aaagagatgg
    36781 gcaccgggct ggtggtgacg gagctgatgg gccagggcgt gagtgccatt accggtgatt
    36841 attcccgtgg tgcagcgggc ttctgggtag agaatggcga aattcagtat ccggtgagcg
    36901 aaatcaccat cgcaggtaat ttaaaagata tgtggcgtaa tatcgttgct gttggtaatg
    36961 atattgaaac acgcagtaac atacagtgtg gttcagtgct gttgccggag atgaaaatcg
    37021 ccgggcagta aaaaaactgg cgcgacctgc cgcgccaaat ctccataata aaaaaggaag
    37081 tgagcaatgc gtaaaagcct gttagctatt cttg
//
LOCUS       DAMZFT010000032        37073 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.032, whole genome shotgun sequence.
ACCESSION   DAMZFT010000032 DAMZFT010000000
VERSION     DAMZFT010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 37073)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37073)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37073
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.032"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..173
                     /locus_tag="PN977_001758"
     CDS             <1..173
                     /locus_tag="PN977_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HDI6041800.1"
                     /translation="TSMSDIVRDLSRLGWTDERIGTELGMDQDEVLRLKQISGLTELF
                     QEENFSPAWTVR"
     gene            complement(544..2690)
                     /locus_tag="PN977_001759"
                     /pseudo
     CDS             complement(544..2690)
                     /locus_tag="PN977_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310819.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
     gene            4362..4907
                     /gene="cobU"
                     /locus_tag="PN977_001760"
     CDS             4362..4907
                     /gene="cobU"
                     /locus_tag="PN977_001760"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HDI6041801.1"
                     /translation="MMILVTGGARSGKSRHAEALIGDSSQVLYIATSQILDDEMAARI
                     EHHRQGRPAHWRTVERWQHLDELIHADINPHEAVLLECVTTMVTNLLFDYGGDKDPDE
                     WDYQAMEQAINAEIQSLIAACQRCPAKVVLVTNEVGMGIVPESRLARHFRDIAGRVNQ
                     QLAAAANEVWLVVSGIGVKIK"
     gene            4904..5647
                     /gene="cobS"
                     /locus_tag="PN977_001761"
     CDS             4904..5647
                     /gene="cobS"
                     /locus_tag="PN977_001761"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HDI6041802.1"
                     /translation="MSKLFWAMLSFITRLPVPRRWSQGLDFEHYSRGIITFPLIGLLL
                     GAISGLVFMVLQAWCGVPLAALFSVLVLALMTGGFHLDGLADTCDGVFSARSRDRMLE
                     IMRDSRLGTHGGLALIFVVLAKILVLSELALRGEPILASLAAACAVSRGTAALLMYRH
                     RYAREEGLGNVFIGKIDGRQTCVTLGLAAIFAAVLLPGMHGVAAMVVTMVAIFILGQL
                     LKRTLGGQTGDTLGAAIELGELVFLLALL"
     gene            5659..6738
                     /gene="cobT"
                     /locus_tag="PN977_001762"
     CDS             5659..6738
                     /gene="cobT"
                     /locus_tag="PN977_001762"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HDI6041803.1"
                     /translation="MQTLADLLNTIPAIDPAAMSRAQRHIDGLLKPVGSLGRLEALAI
                     QLAGMPGLNGIPHVGKKAVLVMCADHGVWEEGVAISPKEVTAIQAENMTRGTTGVCVL
                     AAQAGANVYVIDVGIDTAEPIPGLINMRVARGSGNIASAPAMSRHQAEKLLLDVICYT
                     RELAKNGVTLFGVGELGMANTTPAAAIVSTITGRAPEEVVGIGANLPTDKLANKIDVV
                     RRAITLNQPNPQDGVDVLAKVGGFDLVGMAGVMLGAASCGLPVLLDGFLSYAAALAAC
                     QMSPAIKPYLIPSHLSAEKGARIALSHLGLEPFLNMEMRLGEGSGAALAMPIIEAACA
                     IYNNMGELAASNIVLPGNTTSDLNS"
     gene            6800..7735
                     /gene="ldtA"
                     /locus_tag="PN977_001763"
     CDS             6800..7735
                     /gene="ldtA"
                     /locus_tag="PN977_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HDI6041804.1"
                     /translation="MMRRVNIFCSFALLFASHTSLAVTYPLPPEGSRLVGQSFTVTVP
                     DHNTQPLETFAAQYGQGLSNMLEANPGADVFLPKSGSQLTIPQQLILPATVRKGIVVN
                     VAEMRLYYYPPDSNTVEVFPIGIGQAGRETPRNWVTTVERKQEAPTWTPTPNTRREYA
                     KRGESLPAFVPAGPDNPMGLYAIYIGRLYAIHGTNANFGIGLRVSQGCIRLRNDDIKY
                     LFDNVPVGTRVQIIDQPVKYTTEPDGSNWLEVHEPLSRNRAEYESDRKVPLPVTPSLR
                     AFISGQEVDVNRANAALQRRSGMPVQISSGSRQMF"
     gene            complement(7791..7866)
                     /locus_tag="PN977_001764"
     tRNA            complement(7791..7866)
                     /locus_tag="PN977_001764"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7831..7833),aa:Asn,seq:gtt)
     gene            8193..9110
                     /gene="nac"
                     /locus_tag="PN977_001765"
     CDS             8193..9110
                     /gene="nac"
                     /locus_tag="PN977_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen assimilation transcriptional regulator
                     NAC"
                     /protein_id="HDI6041805.1"
                     /translation="MNFRRLKYFVKIVDIGSLTQAAEVLHIAQPALSQQVATLEGELN
                     QQLLIRTKRGVTPTDAGKILYTHARAILRQCEQAQLAVHNVGQALSGQVSIGFAPGTA
                     ASSITMPLLQAVRAEFPEIVIYLHENSGAVLNEKLINHQLDMAVIYEHSPVAGVSSQA
                     LLKEDLFLVGTQDCPGQSVDVNAIAKMNLFLPSDYSAIRLRVDEAFSLRRLTAKVIGE
                     IESIATLTAAIASGMGVAVLPESAARSLCGAVNGWMSRITTPSMSLSLSLNLPARANL
                     SPQAQAVKELLMSVISSPVMEKRQWQLVS"
     gene            9212..10162
                     /gene="cbl"
                     /locus_tag="PN977_001766"
     CDS             9212..10162
                     /gene="cbl"
                     /locus_tag="PN977_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator Cbl"
                     /protein_id="HDI6041806.1"
                     /translation="MNFQQLKIIREAARQDYNLTEVANMLFTSQSGVSRHIRELEDEL
                     GIEIFVRRGKRLLGMTEPGKALLVIAERILNEASNVRRLADLFTNDTSGVLTIATTHT
                     QARYSLPEVIKAFRELFPEVRLGLIQGTPQEIATLLQNGEADIGIASERLSNDPQLVA
                     FPWFRWHHSLLVPHDHPLTQISPLTLESIAKWPLITYRQGITGRSRIDDAFARKGLLA
                     DIVLSAQDSDVIKTYVALGLGIGLVAEQSSGEQEEENLIRLDTRHLFDANTVWLGLKR
                     GQLQRNYVWRFLELCNAGLSVEDIKRQVMENSEEEIDYQI"
     gene            complement(10200..10275)
                     /locus_tag="PN977_001767"
     tRNA            complement(10200..10275)
                     /locus_tag="PN977_001767"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10240..10242),aa:Asn,seq:gtt)
     gene            10280..11923
                     /gene="yeeO"
                     /locus_tag="PN977_001768"
     CDS             10280..11923
                     /gene="yeeO"
                     /locus_tag="PN977_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416491.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic metabolite efflux MATE transporter YeeO"
                     /protein_id="HDI6041807.1"
                     /translation="MLRHILTAKNLLSNPIFKFPNCLPFLSTVCCICRQFVGENLCSF
                     ADSPSLFEMWFHFLQLRSALNISSALRQVVHGTRWHAKRKSYKVLFWREITPLAVPIF
                     MENACVLLMGVLSTFLVSWLGKDAMAGVGLADSFNMVIMAFFAAIDLGTTVVVAFSLG
                     KRDRRRARVATRQSLVIMTLFAVLLATLIHHFGEQIIDFVAGDATIEVKALALTYLEL
                     TALSYPAAAITLIGSGALRGAGNTKIPLLINGSLNILNIIISGILIYGLFSWPGLGFV
                     GAGLGLTISRYIGAVAILWVLAIGFNPALRISLKSYFKPLNFSIIWEVMGIGIPASVE
                     SVLFTSGRLLTQMFVAGMGTSVIAGNFIAFSIAALINLPGSALGSASTIITGRRLGVG
                     QIAQAEIQLRHVFWLSTLGLTAIAWLTAPFAGVMASFYTQDPQVKHVVVILIWLNALF
                     MPIWSASWVLPAGFKGARDARYAMWVSMLSMWGCRVVVGYVLGIMLGWGVVGVWMGMF
                     ADWAVRAVLFYWRMVTGRWLWKYPRSEPQKCEKKPAVSE"
     gene            12024..12099
                     /locus_tag="PN977_001769"
     tRNA            12024..12099
                     /locus_tag="PN977_001769"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:12057..12059,aa:Asn,seq:gtt)
     gene            complement(12551..13267)
                     /locus_tag="PN977_001770"
     CDS             complement(12551..13267)
                     /locus_tag="PN977_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDI6041808.1"
                     /translation="MGRKWANIVAKKTAKDGATSKIYAKFGVEIYAAAKQGEPDPELN
                     TSLKFVIERAKQAQVPKHVIDKAIDKAKGGGDETFVQGRYEGFGPNGSMIIAETLTSN
                     VNRTIANVRTIFNKKGGNIGAAGSVSYMFDNTGVIVFKGTDPDHIFEILLEAEVDVRD
                     VTEEEGNIVIYTEPTDLHKGIAALKAAGITEFSTTELEMIAQSEVELSPEDLEIFEGL
                     VDALEDDDDVQKVYHNVANL"
     gene            complement(13610..15064)
                     /gene="amn"
                     /locus_tag="PN977_001771"
     CDS             complement(13610..15064)
                     /gene="amn"
                     /locus_tag="PN977_001771"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP nucleosidase"
                     /protein_id="HDI6041809.1"
                     /translation="MNNKGSGLTPAQALDKLDALYEQSVVALRNAIGNYITSGELPDE
                     NARKQGLFVYPSLTVTWDGSTTNPPKTRAFGRFTHAGSYTTTITRPTLFRSYLNEQLT
                     LLYQDYGAHISVQPSQHEIPYPYVIDGSELTLDRSMSAGLTRYFPTTELAQIGDETAD
                     GIYHPTEFSPLSHFDARRVDFSLARLRHYTGTPVEHFQPFVLFTNYTRYVDEFVRWGC
                     SQILDPDSPYIALSCAGGNWITAETEAPEEAISDLAWKKHQMPAWHLITADGQGITLV
                     NIGVGPSNAKTICDHLAVLRPDVWLMIGHCGGLRESQAIGDYVLAHAYLRDDHVLDAV
                     LPPDIPIPSIAEVQRALYDATKLVSGRPGEEVKQRLRTGTVVTTDDRNWELRYSASAL
                     RFNLSRAVAIDMESATIAAQGYRFRVPYGTLLCVSDKPLHGEIKLPGQANRFYEGAIS
                     EHLQIGIRAIDLLRAEGDRLHSRKLRTFNEPPFR"
     gene            complement(15166..16482)
                     /gene="shiA"
                     /locus_tag="PN977_001772"
     CDS             complement(15166..16482)
                     /gene="shiA"
                     /locus_tag="PN977_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate transporter"
                     /protein_id="HDI6041810.1"
                     /translation="MDSTLISTRPDEGTLSLSRARRAALGSFAGAVVDWYDFLLYGIT
                     AALVFNREFFPQVSPAMGTLAAFATFGVGFLFRPLGGVIFGHFGDRLGRKRMLMLTVW
                     MMGIATALIGILPSFSTIGWWAPILLVTLRAIQGFAVGGEWGGAALLSVESAPKNKKA
                     FYSSGVQVGYGVGLLLSTGLVSLISMMTTDEQFLSWGWRIPFLFSIVLVLGALWVRNG
                     MEESAEFEQQQHYQAAAKKRIPVIEALLRHPGAFLKIIALRLCELLTMYIVTAFALNY
                     STQNMGLPRELFLNIGLLVGGLSCLTIPCFAWLADRFGRRRVYITGALIGTLSAFPFF
                     MALEAQSIFWIVFFSIMLANIAHDMVVCVQQPMFTEMFGASYRYSGAGVGYQVASVVG
                     GGFTPFIAAALITYFAGNWHSVAIYLLAGCLISAMTALLMKDNQRA"
     gene            16797..17849
                     /locus_tag="PN977_001773"
     CDS             16797..17849
                     /locus_tag="PN977_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HDI6041811.1"
                     /translation="MFLASLLRRIAFSYYDYKAYNFNIEKTDFVVIHIPDQIGDAMAI
                     FPVIRALELHKIKHLLIVTSTINLEVFNALKLEKTKLTLVTMTMQDHATLKEIKDLAK
                     NITQKYGTPDLCIEAMRKKNLKTMIFISQLKAKTNFQVVGLTMKCYSPLCKNASRMDQ
                     NLRAPVPMTWAFMMREAGFPAVRPIYELPLSEDVLDEVREEMRSLGSYIALNLEGSSQ
                     ERTFSLSIAENLIAKIQSETDIPIVIVHGPKGEDKARVLVDCYNNVYRLSLSPSIKRS
                     AAIIKDAYIAITPDTSILHMASAYNTPVVAIYADYKTRWPAMADVSESVVVGQKIDNI
                     SLDEFAKALKSVLARI"
     gene            complement(18111..25187)
                     /gene="yeeJ"
                     /locus_tag="PN977_001774"
     CDS             complement(18111..25187)
                     /gene="yeeJ"
                     /locus_tag="PN977_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter adhesin YeeJ"
                     /protein_id="HDI6041812.1"
                     /translation="MATKKRSGEEINDRQILCGMGIKLRRLTAGICLITQLAFPMAAA
                     AQGVVNTATQQPVPAQIAIANANTVPYTLGALESAQSVAERFGISVAELRKLNQFRTF
                     ARGFDNVRQGDELDVPAQVSENNLTPPPGNSSGNLEQQIASTSQPIGSLLAEDMNSEQ
                     AANMARGWASSQASGAMTDWLSRFGTARITLGVDEDFSLKNSQFDFLHPWYETPDNLF
                     FSQHTLHRTDERTQINNGLGWRHFTPTWMSGINFFFDHDLSRYHSRAGIGAEYWRDYL
                     KLSSNGYLPLTNWRSAPELDNDYEARPANGWDVRAEGWLPAWPHLGGKLVYEQYYGDE
                     VALFDKDDRQSNPHTITAGLNYTPFPLMTFSAEQRQGKQGENDTRFAVDFTWQPGSAM
                     QKQLDPNEVAARRSLAGSRYDLVDRNNNIVLEYRKKELVRLTLTDPVTGKSGEVKSLV
                     SSLQTKYALKGYNVEATALEAAGGKVVTTGKDILVTLPAYRFTSTPETDNTWPIEVTA
                     EDVKGNLSNREQSMVVVQAPTLSQKDSSVSLSTQTLSADSHSTATLTFIAHDAAGNPV
                     IGLVLSTRHEGVQDITLSEWKDNGDGSYTQILTTGAMSGTLTLMPQLNGVDAAKAPAV
                     VNIISISSSRTHSSIKIDKDRYLSGNPIEVTVELRDENDKPVKEQKQQLNNAVSIDNV
                     KPGVTTDWKETADGVYKATYTAYTKGSGLTAKLLMQNWNEDLHTAGFIIDANPQSAKI
                     ATLSASNNGVLANENAANTVSVNVADEGSNPINDHTVTFAVLSGSATSFNNQNTAKTD
                     VNGLATFDLKSSKQEDNTVEVTLENGVKQTLIVSFVGDSSTAQVELQKSKNEVVADGN
                     DSATMTATVRDAKGNLLNDVKVTFNVNSAEAKLSQTEVNSHDGIATATLTSLKNGDYR
                     VTASVSSGSQANQQVIFIGDQSTAALTLSVPSGDITVTNTAPLHMTATLQDKNGNPLK
                     DKEITFSVPNDVASRFSISNSGKGMTDSNGTAIASLTGTLAGTHMITARLANSNVSDT
                     QPMTFVADKDRAVVVLQTSKAEIIGNGVDETTLTATVKDPSNHPVAGITVNFTMPQGV
                     AANFTLENNGIAITQANGEAHVTLKGKKAGTHTVTATLGNNNTSDSQPVTFVADKTSA
                     QVVLQMSKDEITGNGVDNATLTATVKDQFDNEVNNLPVTFSSASSGLTLTPGVSNTNE
                     SGIAQATLAGVAFGEQTVTASLANNGASDNKTVHFIGDTAAAKIIELTPVPDSIIAGT
                     PQNSSGSVITATVVDNNGFPVKGVTVNFTSRTNSAEMTNGGQAVTNEQGKATVTYTNT
                     RSSIESGARPDTVEASLENGSSTLSTSINVNADASTAHLTLLQALFDTVSAGDTTNLY
                     IEVKDNYGNGVPQQEVTLRVSPSEGVTPSNNAIYTTNHDGNFYASFTATKAGVYQVTA
                     TLENGDSMQQTVTYVPNVANAEITLAASKDPLIADNNDLTTLTATVADTEGNAIANTE
                     VTFTLPEDVKANFTLSDGGKAITDAEGKAKVTLKGTKAGAHTVTASMTGGKSEQLVVN
                     FIADTLSAQVNLNVTEDNFIANNVGMTTLQATVTDGNGNPLANEAVTFTLPADVSASF
                     TLGQGGSAITDINGKAEVTLSGTKSGTYPVTVSVNNYGVSDTKQVTLIADAGTATLAS
                     LTSVYSFVVSTTEGATMTASVTDANGNPVEGIKVNFRGTSVTLSSTSVETDDQGFAEI
                     LVTSTEVGLKTVSASLADKPTEVISRLLNAKADINSATITSLEIPEGQLMVAQDVAVK
                     AHVNDQFGNPILNESVTFSAEPPEHMTISQNIVSTDTHGIAEVSMTPERNGSYMVKAS
                     LANGASLEKQLEAIDEKLTLTASSPLIGVYAPTGTTLTATLTSANGTPVEGQVINFSV
                     TPEGATLSGGKVRTNSSGQAPVVLTSNKVGTYTVTASFHNGVTIQTQTTVKVTGNSST
                     AHVASFIADPSTIAATNSDLSTLKATVEDGSGNLIEGLTVYFALKSGSATLTSLTAVT
                     DQNGIATTSVKGAMTGSVTVSAVTTAGGMQTVDITLVAGPADTSQSVLKSNRSSLKGD
                     YTDSAELRLVLHDISGNPIKVSEGMEFVQSGTNVPYIKISAIDYSLNINGDYKATVTS
                     GGEGIATLIPVLNGVHQAGLSTTIQFTRAEDKIMSGTVSVNGTDLPTTTFPSQGFTGA
                     YYQLNNDNFAPGKTAADYEFSSSASWVDVDATGKVTFKNVGSNWERITATPKSGGPSY
                     VYEIRVKSWWVNAGEAFMIYSLAENFCSSNGYTLPRANYLNHSSSRGIGSLYSEWGDM
                     GHYTTDAGFQSNMYWSSSPANSSEQYVVSLATGDQSVFEKLGFAYATCYKNL"
     gene            complement(25501..25576)
                     /locus_tag="PN977_001775"
     tRNA            complement(25501..25576)
                     /locus_tag="PN977_001775"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(25541..25543),aa:Asn,seq:gtt)
     gene            complement(25677..26474)
                     /gene="mtfA"
                     /locus_tag="PN977_001776"
     CDS             complement(25677..26474)
                     /gene="mtfA"
                     /locus_tag="PN977_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DgsA anti-repressor MtfA"
                     /protein_id="HDI6041813.1"
                     /translation="MIKWPWKVQESAHQTALPWQEALSIPLLTCLTEQEQSKLVTLAE
                     RFLQQKRLVPLQGFELDSLRSCRIALLFCLPVLELGLEWLDGFHEVLIYPAPFVVDDE
                     WEDDIGLVHNQRIVQSGQSWQQGPIVLNWLDIQDSFDASGFNLIIHEVAHKLDTRNGD
                     RASGVPFIPLREVAGWEHDLHAAMNNIQEEIELVGDNAASIDAYAASDPAECFAVLSE
                     YFFSAPELFAPRFPSLWQRFCQFYQQDPLQRLHRTNDTDSFSATNVH"
     gene            26568..26657
                     /locus_tag="PN977_001777"
     tRNA            26568..26657
                     /locus_tag="PN977_001777"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:26602..26604,aa:Ser,seq:cga)
     gene            complement(27227..27757)
                     /locus_tag="PN977_001778"
     CDS             complement(27227..27757)
                     /locus_tag="PN977_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416483.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HDI6041814.1"
                     /translation="MNRFSKTQIYLHWITLLFVAITYAAMELRGWFPKGSSTYLLMRE
                     THYNAGIFVWVLMFSRLIIKHRYSDPSIVPPPPAWQMKAASLMHIMLYITFLALPLLG
                     IALMAYSGKSWSFLGFNVSPFVTPNSEIKALIKNIHETWANIGYFLIAAHAGAALFHH
                     YIQKDNTLLRIMPRRK"
     gene            complement(28100..28750)
                     /gene="zinT"
                     /locus_tag="PN977_001779"
     CDS             complement(28100..28750)
                     /gene="zinT"
                     /locus_tag="PN977_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-binding protein ZinT"
                     /protein_id="HDI6041815.1"
                     /translation="MAIRLHKLAVALGVFIVSAPAFSHGHHSHGKPLTEVEQKAANGV
                     FDDANVQNRTLSDWDGVWQSVYPLLQSGKLDPVFQKKADADKTKTFAEIKDYYHKGYA
                     TDIEMIGIEDGIVEFHRNNETTSCKYEYDGYKILTYKSGKKGVRYLFECKDPESKAPK
                     YIQFSDHIIAPRKSSHFHIFMGNDSQQSLLNEMENWPTYYPYQLSSEEVVEEMMSH"
     gene            complement(29007..29642)
                     /gene="msrQ"
                     /locus_tag="PN977_001780"
     CDS             complement(29007..29642)
                     /gene="msrQ"
                     /locus_tag="PN977_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase
                     heme-binding subunit MsrQ"
                     /protein_id="HDI6041816.1"
                     /translation="MRLTAKQVTWLKVSLHLAGLLPFLWLVWAINHGGLGADPVKDIQ
                     HFTGRTALKFLLATLLITPLARYVKQPLLIRTRRLLGLWCFAWATLHLTSYALLELGV
                     NNLALLGKELITRPYLTLGIISWVILLALAFTSTQAMQRKLGKHWQQLHNFVYLVAIL
                     APIHYLWSVKIISPQPLIYAGLAVLLLALRYKKLRSLFNRLRKQVHNKLSV"
     gene            complement(29643..30647)
                     /gene="msrP"
                     /locus_tag="PN977_001781"
     CDS             complement(29643..30647)
                     /gene="msrP"
                     /locus_tag="PN977_001781"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase catalytic
                     subunit MsrP"
                     /protein_id="HDI6041817.1"
                     /translation="MKKNQFLKESDVTAESVFFMKRRQVLKALGISAAALSLPHAAHA
                     DLLSWFKGNDRPPAPAGKPLEFSKPAAWQNNLPLTPVDKVSGYNNFYEFGLDKADPAA
                     NAGSLKTDPWTLKISGEVAKPLTLDHDDLTRRFPLEERIYRMRCVEAWSMVVPWIGFP
                     LHKLLALAEPTSNAKYVAFETIYAPEQMPGQQDRFIGGGLKYPYVEGLRLDEAMHPLT
                     LMTVGVYGKALPPQNGAPVRLIVPWKYGFKGIKSIVSIKLTRERPPTTWNLAAPDEYG
                     FYANVNPHVDHPRWSQATERFIGSGGILDVQRQPTLLFNGYADQVASLYRGLDLRENF
                     "
     gene            complement(30756..31169)
                     /gene="hiuH"
                     /locus_tag="PN977_001782"
     CDS             complement(30756..31169)
                     /gene="hiuH"
                     /locus_tag="PN977_001782"
                     /EC_number="3.5.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyisourate hydrolase"
                     /protein_id="HDI6041818.1"
                     /translation="MLKRYLVLSVVTAAFSLPSLVYAAQQNILSVHILNQQTGKPAAD
                     VTVTLEKKADNGWLQLNTAKTDKDGRIKALWPEQTATTGDYRVVFKTGDYFKKQNLES
                     FFPEIPVEFLINKVNEHYHVPLLLSQYGYSTYRGS"
     gene            31302..31973
                     /gene="hprR"
                     /locus_tag="PN977_001783"
     CDS             31302..31973
                     /gene="hprR"
                     /locus_tag="PN977_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor HprR"
                     /protein_id="HDI6041819.1"
                     /translation="MKILLIEDNQRTQEWVTQGLSEAGYVIDAVSDGRDGLYLALKDD
                     YALIILDIMLPGMDGWQILQTLRTAKQTPVICLTARDSVDDRVRGLDSGANDYLVKPF
                     SFSELLARVRAQLRQHHALNSTLEISGLRMDSVSQSVSRDNISITLTRKEFQLLWLLA
                     SRAGEIIPRTVIASEIWGINFDSDTNTVDVAIRRLRAKVDDPFPEKLIATIRGMGYSF
                     VAVKK"
     gene            31973..33331
                     /gene="hprS"
                     /locus_tag="PN977_001784"
     CDS             31973..33331
                     /gene="hprS"
                     /locus_tag="PN977_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     HprS"
                     /protein_id="HDI6041820.1"
                     /translation="MKRLSITVRLTLLFILLLSVAGAGIVWTLYNGLASELKWRDDTT
                     LINRTAQIKQLLIDGVNPDTLPVYFNRMMDVSQDILIIHGDGINKIVNRTNVSDDMLN
                     NIPASETISAAGIYRSIINDTEIDALRINIDEVSPSLTVTVAKLASARHNMLEQYKIN
                     SIIICIVAIVLCSVLSPLLIRTGLREIKKLSGVTEALNYNDSREPVEVSALPRELKPL
                     GQALNKMHQALVKDFERLSQFADDLAHELRTPINALLGQNQVTLSQTRSIAEYQKTIA
                     GNIEELENISRLTENILFLARADKNNVLVKLDSLSLNKEVENLLDYLEYLSDEKEICF
                     KVECNQQIFADKILLQRMLSNLIVNAIRYSPEKSHIHITSFLDTNGYLNIDVASPGTK
                     IHEPEKLFRRFWRGDNSRHSVGQGLGLSLVKAIAELHGGSATYHYLNKHNVFRITLPQ
                     RN"
     gene            complement(33438..34289)
                     /gene="hchA"
                     /locus_tag="PN977_001785"
     CDS             complement(33438..34289)
                     /gene="hchA"
                     /locus_tag="PN977_001785"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein deglycase HchA"
                     /protein_id="HDI6041821.1"
                     /translation="MTVQTSKNPQVDIAEDNAFFPSEYSLSQYTSPVSDLDGVDYPKP
                     YRGKHKILVIAADERYLPTDNGKLFSTGNHPIETLLPLYHLHAAGFEFEVATISGLMT
                     KFEYWAMPHKDEKVMPFFEQHKSLFRNPKKLADVVASLNADSEYAAIFVPGGHGALIG
                     LPESQDVAAALQWAIKNDRFVISLCHGPAAFLALRHGDNPLNGYSICAFPDAADKQTP
                     EIGYMPGHLTWYFGEELKKMGMNIINDDITGRVHKDRKVLTGDSPFAANALGKLAAQE
                     MLAAYAG"
     gene            complement(34881..36044)
                     /gene="ompC"
                     /locus_tag="PN977_001786"
     CDS             complement(34881..36044)
                     /gene="ompC"
                     /locus_tag="PN977_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001080680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HDI6041822.1"
                     /translation="MKRKVLAMLVPALLVAGAANAAEIYNKNGNKLDLYGKVDARHTF
                     SDNPGDDGDETIIELGFKGETQITDQLTGYGQALTKTKASDTEGSDNTYVKLAFAGLK
                     FGEMGSFDYGRNYGVIYDVEAWTDMLPVFGGDSYTWTDNFMAGRANGVATYRNSDFFG
                     LVEGLNFALQYQGNNEGSNAGEDQEGTKNGHEDVRFQNGDGFGLSTSYDFDFGLSLGA
                     AYSNSDRTNSQVALGGYHYNEYSKFAGGDTAEAWTFGAKYDANNVYLAMMYAETRNMT
                     PYGNVGIANKTQNFEAVAQYQFDFGLRPSLAYVYSKGKDLGGNDYNNNGHQEYVDQDL
                     VNYVEIGATYYFNKNFSTYVDYKINLLDKDDDFYDNNGIATDDVVGVGLVYQF"
     gene            36612..36977
                     /locus_tag="PN977_001787"
     CDS             36612..36977
                     /locus_tag="PN977_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HDI6041823.1"
                     /translation="MEKCDFYHIIVLSLNFPGYLKMEYGSTKMEERLSRSPGGKLALW
                     AFYTWCGYFVWAMARYIWVMSRIPDAPVSGFESDLGSTAGKWLGALVGFLFMALVGAL
                     LGSIAWYTRPRPARSRRYE"
BASE COUNT         9726 a         8086 c         8890 g        10371 t
ORIGIN      
        1 taacgtcaat gtctgacatt gtcagggacc tttcgcgtct gggctggaca gatgagcgta
       61 ttggcacaga gttgggcatg gatcaggatg aagtgctgag gctcaaacag attagcgggc
      121 tgacagagct gtttcaggaa gagaatttca gcccggcatg gacggttcgt tgatattgta
      181 aaatatgata gttggagtag gggacaatag aaggagaata tttattccac cttcttccgc
      241 gtcgggataa atcaaaaggt attatccatt gattcagtat ctgggggaga gtaaataatc
      301 taaaaatagt attgcgttgt gaactgatat cacttttgaa ttacaaaggt ataggctcag
      361 tagccagctt tataagtcat tgtcgtagca atgagtatag aatcatgctg gcgggaagaa
      421 taaatattca tctacagatg gaaaacaacg acgaaaaacc ttcaacaaac acaatacgtt
      481 cgttagcctg cccgcaaagc gggcagatca ggacaaagaa ttccataacg attgtttttg
      541 tcatcagaaa ttccatacaa aacctatagc tgcattaaaa cctgctgcag gaatagtttc
      601 atgtgctgta cggtagcgtt tgtctgtcag gttattgagt gccaggttaa tccggtactg
      661 atcctcgtta ccgaattctg tatttactgc aaagttgaga gtggcccagc ccggaatatt
      721 tgtttcggta ccgttactgt catcttttgc actagaggca gcacgaataa aaacatcaga
      781 gattatgttg gcctgaccca tcacaagagt atgtttcagc cctatacgtc cgtttatcgc
      841 tggttcacct gtattagttg tttttaatgt tgaagtttca tattgccgac gaattaaatt
      901 gccactgata tatggcgaga aaacccagcc attatattct gcacttattt ccagtcccca
      961 tgtttttgcc cgatcaatat tgtcataata atagtagcta ctacgggagg agttggtgtt
     1021 accattgcaa actatactgc catcacagat cagacttgca atataatctt tagcttctga
     1081 gtagtaaact gcgctgtcaa tcagccactg attaccatta tatcgtgcac ctaattcaaa
     1141 gttattggag tgttcagcct taagatcagg atttccgtat gtgacactgc cgcccgcaga
     1201 tgtctgcata aaaagctggg agagtgtggg aaatacgtag ccttgcgcga acgcagcgcg
     1261 taactccagg ttatcgaaac ctgaatagcg caggcttgta gatgttacca tttcgtgatc
     1321 actcgcagac tctctggcaa gagaggtatc gcttataatg cctgcggtat atgatactcc
     1381 gtcaccacgc gtcaactttg aagaaagcca gtattggcga actcccattg tccatgtcca
     1441 gtgatcggcg aattgccagt cattttgtgc gaatagtgag actgtacttt gctctgactc
     1501 ataataggag cgagttcgtg tttccttatt aatgaagccg gtcagagatt tgcttgaggt
     1561 catgccaccg gacctttggt tgaccctgtc ttgtttgtac tgtgcaccgg taacaagttc
     1621 attattagca ggcagcgaaa agtgactctg caatgtgacc gcctgagtgt attgcttatc
     1681 atgggtgtca gtcttgttat gaacggtcag agcctgaatc atcggactgg gaacaggctg
     1741 tgtcgttttt acttcgttgg caaattggcg ctggatggtc tgctcatacg cgtcgaaatg
     1801 aattttttta gatagtcacc gtccacgtct gtgtcataga ataccccaac tttctctcgt
     1861 tcaagtttag gtattttgac actaaatgcc tcataacttc cgtctggatc ctcatagtaa
     1921 gtttgtgtcg cgagtctgta gcgatcaagc gagaggccaa aacgatggtt tccggagtta
     1981 taacccaacc atacaccctg actattgtta cgatagttgg tattcggcag acgtccatcc
     2041 ggcgtatcac gattgccctg atcagaataa ctaccgttga tgcgataatc aaatccaccg
     2101 atgctcccct ggaccgcgat tgattcttcc cagcctgctg ttgcggaatt ataaacagct
     2161 ttcacaactc cagatgcaag tttgttacct ccctttttgg tgatgaagtt aacaataccg
     2221 ccaattgcct gtgaaccgta cagtacggaa tatggacctt tcactatctc aacacgctcc
     2281 agcgcagact catctatcaa cagtcccaca ctataattat ctccggcgcg ctgataagtt
     2341 acctcctgac catcaatgag aattaaaaca cgggaggatg cttcgccacg aatgcggatt
     2401 tgtttacggc ctgccaagga gttgtctgtt atctctactc cggggatgtc ctgcaaatta
     2461 tcggctatgg aggcattggt ggagttctgc aatgtttgtt ggtcaataac ctgtatagtc
     2521 gccgggcttt cccaaaggtt gcttgccgaa cgattcgtgg tcacgacgag cgtctcttcg
     2581 ttgctgattt ttgtttctga agaagttgcg gttgcagtag aaactatccc agaaatgcat
     2641 aatgacagag tggaaaggat ataaattcga caaggtatat tatataacat ttcatccctc
     2701 attaatacaa agtacaaatg attgctgtaa tgataattat tttcatttat gttctcatat
     2761 atctataatt attgtcatct tcataaattg taaatatcaa acatttttga ccttaatcat
     2821 gttttttgat agatatttta cgactgaaca tatccgtcgt ttcctctgac tcgtgtcagt
     2881 cggtctctgg ttgtatccac ggaccgcact cttgacataa gagtgcggtc cgtggaaaac
     2941 ggagttgaaa aaatggtgtc tgaaagaagg taccatcatt gtgatgacaa atactttggc
     3001 gtggataatg taattaattg ctgaaattga ccgtatatgc ggtaagactc atatcagtat
     3061 caggtccggt taattgctga tactaatata aactttttac aaaaggatga atgcagaagg
     3121 ttactctaat ggccagagtg atgacaatga tggtcagacg ttgacctgca gaaactgcat
     3181 tttaattggt ctataaagtc tgcaagagga atcagggtca gatcagtgag tcttatcgcg
     3241 gcccgaagct atgactgaaa gaaggctgga tacagaattg caacaatttg gcaatgtcag
     3301 aacacagaag ctttgcaagc aggtgaaagg ttatgtctag caaaaggagt atatcagaat
     3361 atatctactg atgtacaaaa cacaacaata gcttcatgat atataaatac gtgatgacga
     3421 gtggtactcc gcatggttat ttaacttctc agcaccacta atgatcctga gcaacttcgt
     3481 atttgggcca ttaccatgga gtagaaagct gtgtctgaga atgcagaatt tgagaaaaga
     3541 attcgctttc ttggagagct tctgaaaatg gcacgctaac aacgttttgt taaaaaatgt
     3601 gaatcacttg ccggggctgc attttctatt cgaggagaat gttgacgtag atattacaga
     3661 aatctcagcg catcaggata aactgctacc ttaggtcaac caccaaaaga aaatacaata
     3721 cgtccagttt gtatgccttt aataacacat cttctaaatg ttgaagaatt atcccgcctg
     3781 ctgaagaacg ttgctctgag tgtgataaac ctttttattc ttgatgatgt cagcgagaag
     3841 atggaatata tccccgactg ggtcgtggtt acccggttaa acatctggtg gcgctaataa
     3901 atctggcaag tcacattctg ttgaatgctg tgacagtgcc ttaccgagag agcgaaattg
     3961 tggtgttcta gtgcatagtc gccaccattc cggataattc tattacgttg ttttacaagt
     4021 tcttttacag cgcagatctg tcgctgacgt tgaatcaata gcctggattt cagaacacaa
     4081 tgcccgtttg ctggttgttc cggcagtagc attaattcat tatctgaatg aaatgtgtca
     4141 atccacagaa accgctgtca atgacgcatt ttgtcggacg ttaacacaag aaagtctggc
     4201 tattccgtaa tgccatctta tttcgtaatg gattgagaat gctggatttt taattatatt
     4261 acgcgcagta ttaatcgcgt aaatgttttg cggtcagcaa cggtggggga tccattgttc
     4321 tgttcaatga ataccatcat acatttgaat caggagtcgg tatgatgatt ttggtgacgg
     4381 ggggcgcacg gagcgggaag agtcgccacg cagaggcgct tattggggac tcttcacagg
     4441 ttctgtatat cgctacctcg caaatccttg atgatgagat ggctgcacgg atagaacatc
     4501 atcggcaagg ccgcccggcg cactggcgca cggtggagcg ttggcaacat cttgatgaat
     4561 taattcatgc agacattaac ccgcatgagg ctgtgttgct ggaatgcgtt accaccatgg
     4621 tgactaatct gttgtttgat tatggcggcg ataaagaccc tgatgaatgg gattatcagg
     4681 cgatggaaca ggcgattaat gctgagattc agtcgttgat tgctgcctgc caacgttgtc
     4741 ccgcaaaggt tgtattagtg actaacgaag tgggaatggg gattgtgccg gagagtcgtc
     4801 tggcacgaca ttttcgtgat attgccgggc gggtaaatca gcagctggcc gctgcggcaa
     4861 atgaagtatg gttggtggtt tcgggtattg gagtaaaaat caaatgagta aattattttg
     4921 ggcgatgctc tcttttatta cgcgtctgcc tgtaccgcgt cgctggtccc agggactgga
     4981 tttcgagcat tattctcgcg gtattattac ttttccttta attggattat tgcttggcgc
     5041 gattagcggg ctggtcttca tggtgctgca ggcatggtgt ggcgtaccac tggcggcact
     5101 gtttagcgtg cttgtgctgg cgctgatgac cgggggattc cacctggacg gcctcgccga
     5161 tacctgtgat ggcgtatttt ctgcacgtag ccgcgatcgc atgttggaaa tcatgcgtga
     5221 tagccgttta ggcacccacg gcggtctggc attgattttt gtggtactgg caaagattct
     5281 ggtgttaagc gagttggctc tgcgtggcga accgatcctt gcatcgctgg cggcagcatg
     5341 tgcggtcagc cgcgggactg ccgcattatt gatgtatcgt catcgttatg cccgggaaga
     5401 agggcttggc aatgtgttca ttggcaaaat tgatgggcga caaacctgcg tcacactcgg
     5461 cttagccgct atttttgctg ccgtattatt gccgggtatg catggtgtgg ctgctatggt
     5521 ggtgacgatg gtggcgattt tcatcctcgg tcagcttctc aaacgtacgc tgggcgggca
     5581 aaccggcgat acgctgggag ctgctataga gcttggtgaa ctggtcttcc tgctggcact
     5641 gctataaaga gacgtgacat gcaaacactt gccgatttac tgaatacgat ccctgccatc
     5701 gatcctgccg cgatgtcgcg tgcacaacgg catattgacg ggttactcaa acctgttggt
     5761 agcctgggaa ggctggaggc gcttgccata caactggcag gaatgccggg gttgaatggc
     5821 ataccgcatg tgggcaaaaa agcggtactg gttatgtgtg ccgatcacgg cgtctgggag
     5881 gaaggggtcg ctatttcccc aaaagaagtg acagccattc aggctgaaaa tatgacccgc
     5941 ggaacaaccg gcgtgtgtgt gctggcagca caagcgggcg ctaacgtcta cgtaattgat
     6001 gttggtattg atactgctga gcctatcccc gggcttatca acatgcgtgt tgctcgaggt
     6061 agtggcaata ttgcttcagc tccggcaatg agtcgccatc aggcagaaaa gttgcttttg
     6121 gacgtcatat gttatacgcg ggagctggca aaaaacggtg ttacgctgtt tggtgtaggt
     6181 gaactgggga tggcaaacac gacaccggca gcggcaatag tcagcacaat cactggccgg
     6241 gctcctgaag aagtggttgg gattggcgca aacctgccga cagataaact ggctaataaa
     6301 attgatgttg tgcgccgggc gattacgttg aatcaaccaa atccgcagga tggcgttgat
     6361 gtcctggcaa aagtgggtgg atttgatttg gtcggaatgg ctggcgtgat gttaggcgct
     6421 gcttcctgcg gtttacccgt gttgctggat ggatttcttt cttatgctgc cgcgctcgca
     6481 gcctgccaga tgtctcctgc aatcaaaccg tatctcattc cttctcacct gtcggcagaa
     6541 aaaggggcgc gtattgcgct gtcgcatttg gggctggagc cttttctcaa tatggagatg
     6601 cgtttaggtg aggggagtgg cgcagctctg gcgatgccca tcatcgaagc tgcctgtgcg
     6661 atatataaca acatgggcga acttgctgcc agtaatattg ttctaccggg gaatacgact
     6721 tctgatttga acagttaaaa acgaaaagtc tgataaaagc tatacttaac ggatagcttt
     6781 cgcgacttag gaaagggaca tgatgcgtcg tgtaaatatt ttttgctcat ttgctctgct
     6841 ttttgccagc catactagcc tggcggtaac ttatccatta cctccagagg gtagccgttt
     6901 agtggggcag tcgtttactg taactgttcc tgatcacaat acccagccgc tggagacttt
     6961 tgccgcacaa tacgggcaag ggttaagtaa catgctggaa gcgaacccgg gcgctgatgt
     7021 ttttttgccg aagtctggct cgcaactcac cattccgcag caactgattt tgcccgccac
     7081 tgttcgtaaa gggattgtgg ttaacgtcgc tgagatgcgt ctttattact acccaccaga
     7141 cagtaatact gtggaagtct ttcctattgg tatcggccag gctgggcgag aaaccccgcg
     7201 taactgggtg actaccgttg aacgtaaaca agaagctcca acctggacgc caacgccgaa
     7261 cactcggcgc gaatatgcga aacgagggga gagtttgccc gcatttgttc ctgcgggccc
     7321 cgataatccc atggggctgt acgcgattta tattggcagg ttgtatgcca tccatggtac
     7381 caatgccaat tttggtattg ggctccgggt aagtcagggc tgtattcgtc tgcgcaatga
     7441 cgatatcaaa tatctgtttg ataatgttcc tgttgggacg cgtgtgcaaa ttatcgacca
     7501 gccagtaaaa tacaccactg aaccagatgg ctcgaactgg ctggaagttc atgagccgtt
     7561 gtcgcgcaat cgtgcagaat atgagtctga ccgaaaagtg ccattgccgg taaccccatc
     7621 tttgcgggcg tttatcagcg ggcaagaagt tgatgtaaat cgcgcaaatg ctgcgttgca
     7681 acgtcgatcg ggaatgcctg tgcaaattag ttctggttca agacagatgt tttaagagcg
     7741 ttggtaatta gaaagcaaaa agcctgctag aaagcaggct tttttgaatt tggctcctct
     7801 gactggactc gaaccagtga catacggatt aacagtccgc cgttctaccg actgaactac
     7861 agaggaatcg tgtgaacggg gcgcatatta atggcagtgc atggtgatgt caaagggtga
     7921 aatgcatttt tttgtttgtt tgctgtcaat ttcaacaaag tggtgagctt tcaatcttat
     7981 tgggtaatga accatcgtgg tgcataccct ccttttatag ggcaggggaa cgcgacagct
     8041 gattaaagga gcaaatgacg aatctatcat aatctcttat ttgacagttg gttagcttgt
     8101 acatcaacgc caaaataaaa ctggcaagca tcttgcaatc tggttgtaag taatggcggc
     8161 acttgggccg attcttaaaa accggaggca acatgaactt cagacgcctg aaatacttcg
     8221 taaaaattgt agatattggt agcctgaccc aggctgctga agtattgcat atcgcacaac
     8281 cagcgctcag ccagcaggtt gccacactgg aaggtgagtt aaatcaacaa cttttgatcc
     8341 gtacaaagcg gggcgttacg ccaacagacg ccggaaaaat tctctatacc catgcgcggg
     8401 ccattttacg tcagtgtgaa caggcccaac tggcggtgca taacgttggt caggcattat
     8461 cggggcaagt ctcgattggc tttgcaccag gaaccgctgc gtcatccatc accatgccct
     8521 tattacaggc ggttcgcgct gaatttccgg agatcgttat ctatcttcat gaaaatagtg
     8581 gtgcagtgct taacgaaaaa ttgataaatc accaactcga tatggcggtg atttatgagc
     8641 attcccctgt ggctggtgta tccagtcagg ctttgctgaa agaagatctt tttctggtag
     8701 gtactcaaga ttgcccgggg caaagcgttg atgtgaatgc tattgcgaaa atgaacctct
     8761 ttctccccag tgattacagt gctattagac ttcgtgttga tgaggctttt tccctacggc
     8821 gactcacggc aaaagttatt ggtgaaattg agtctattgc cacgcttacc gcagcgattg
     8881 ccagcggcat gggcgttgca gtattacccg aatcggccgc gcgttcgtta tgtggcgcag
     8941 taaatgggtg gatgtcacgc attaccactc cttccatgag tctctctttg tcattaaatt
     9001 tacccgccag agcgaactta tcgccacagg cacaggcagt gaaagagttg ttaatgtcag
     9061 tgattagttc tccagtgatg gaaaaaaggc agtggcaatt ggtgagctaa gtgttatatc
     9121 ggaatggaat aagatgcggg tttttattat ttgttatgcc gggcattaga ctttaacaat
     9181 aacgggaaat ctgaactgcc cggagtttac cgtgaatttc caacaactaa agataatccg
     9241 cgaggctgca cgtcaggatt acaacctgac agaggttgcg aatatgcttt ttacctcaca
     9301 gtcaggcgtc agccgtcata ttcgggaact ggaggatgaa cttggcatcg aaatatttgt
     9361 tcgacgaggt aagcgactgc tgggcatgac tgaaccgggc aaagcattac tggtcattgc
     9421 agaacgtatt ctgaatgaag ccagtaatgt tcgtcggctt gcagacctgt ttaccaacga
     9481 tacgtctggc gttctcacta ttgcaacgac gcatactcag gcacgttata gcttgccaga
     9541 ggtcattaaa gcttttcgcg aacttttccc ggaggttcgg ctcgggctaa tccaggggac
     9601 gccacaggaa attgcgacat tgttgcaaaa tggcgaagct gatattggta tcgccagcga
     9661 gcgtttgagt aatgacccgc agctcgtcgc cttcccgtgg tttcgttggc accatagttt
     9721 gcttgttcca cacgatcatc ccttgacgca aatttcacca ttgacgctgg aatcaatagc
     9781 gaagtggccg ttaatcactt accgacaggg gattacgggg cgctcacgta ttgatgacgc
     9841 atttgcccgc aaaggtttgc tggcagatat tgtattaagt gcgcaggatt ctgatgtcat
     9901 taaaacctat gttgctcttg ggcttgggat cggattagtt gccgaacaat ccagcggcga
     9961 acaagaggaa gagaatttaa tccgtctgga tacgcggcat cttttcgatg ctaatactgt
    10021 ctggttggga ctgaagcgag gacaacttca gcgtaactat gtctggcgct ttctggaact
    10081 ttgtaatgca ggactgtcag ttgaggatat caagcggcag gtgatggaaa acagtgaaga
    10141 ggaaattgat tatcagatat agcaaaaagc ccgctataaa agcgggcttt tcagaaattt
    10201 ggctcctctg actggactcg aaccagtgac atacggatta acagtccgcc gttctaccga
    10261 ctgaactaca gaggaatcgc tgttgaggca catcttaacg gcgaaaaatc ttttgtcaaa
    10321 cccgattttt aaattcccca actgtttgcc gtttctatca acagtttgtt gcatttgcag
    10381 acaatttgtt ggcgaaaatc tttgcagctt tgctgattct ccctcattat ttgaaatgtg
    10441 gtttcacttt ctgcaattaa ggtcggcttt gaatatctcc tctgctttac gccaggttgt
    10501 tcacggcact cgctggcacg ctaaacgcaa gagctacaaa gtgttgttct ggcgcgagat
    10561 aaccccgctt gctgttccta tcttcatgga gaatgcctgt gtcctgttga tgggtgtcct
    10621 gagcactttt ctggtcagct ggctgggaaa agatgcgatg gccggcgtgg gattggcgga
    10681 cagcttcaat atggtcatta tggctttttt tgctgctatc gatcttggta ctactgtcgt
    10741 tgtggcattt agtctcggta agcgggatcg acgacgagcg agggtggcga cgcggcagtc
    10801 attggtgatc atgacgttgt ttgccgtact gttggcaacg cttattcatc attttggcga
    10861 acaaattatt gatttcgtcg cgggtgatgc cacgatagaa gttaaagcac tggcgttgac
    10921 ttatctggag ctgacggcac tcagttatcc agcagctgcc atcactttga ttggtagcgg
    10981 ggcacttcgt ggtgcaggga atacgaaaat accgctactg attaacggta gcctgaatat
    11041 tcttaatatt attattagcg gcatattgat ttacggcctt ttctcctggc cgggactggg
    11101 atttgtcggg gctgggttgg gtttaaccat ttctcgttat attggcgcag ttgcaatttt
    11161 gtgggtgctg gcgattggtt ttaatcctgc gctaaggatt tcgttaaaga gctattttaa
    11221 accgctgaat tttagcatta tctgggaagt catggggatt ggtattccag cgagtgtcga
    11281 atcagtgtta tttaccagtg gtcggttatt aacccaaatg ttcgttgccg ggatggggac
    11341 cagtgttatt gccggaaatt ttatcgcgtt ttcaattgcg gctcttatca acttacccgg
    11401 aagtgcgctc ggctctgctt ctacgatcat tacaggccga aggttggggg tagggcagat
    11461 agcgcaagca gagattcagt tgcggcatgt gttctggctt tccactcttg gattaacggc
    11521 catcgcctgg ctaacggctc cctttgccgg ggttatggca tcgttttaca cccaggatcc
    11581 acaggttaaa catgtcgttg tgattctgat ttggctaaat gctttattta tgcctatttg
    11641 gtccgcctca tgggtgctac ccgctggatt taaaggtgct cgcgatgccc gttacgccat
    11701 gtgggtttct atgttgagca tgtggggttg ccgggttgtc gtcggttatg tgctgggcat
    11761 catgcttggc tggggtgtgg ttggtgtctg gatgggaatg tttgccgact gggctgtgcg
    11821 ggccgtgctg ttttactggc gaatggttac tggacgttgg ctgtggaaat accctcgatc
    11881 cgaaccgcaa aagtgtgaaa aaaagccagc tgtgtcggaa taaacgacaa aatgcagatt
    11941 atttcagcaa acgatttcaa atttaaaaaa caggctttga cattatgggt gggcatcgct
    12001 aatattcgtc tcgttctcac gattcctctg tagttcagtc ggtagaacgg cggactgtta
    12061 atccgtatgt cactggttcg agtccagtca gaggagccaa attttagttt tgagatatct
    12121 ctatgagtct caaaacagtt aaaaaataaa aattattttt acccggttac cttgtaaaaa
    12181 agtgaccggg ttttttgatt tctgtgtttc gtgcgcttaa tcgagtctca cgcttcagtg
    12241 agcaagttgc ccccaacaga catcacgatt cgcaaggtca aaaactctgt tccatgaagt
    12301 accgtacaaa gataaagaga aaaatgcctt atgcgggatg tcgctatcgc gtctttatcg
    12361 ggaaactatg cgttattgcc actttgcctt ctgtaatcag aaaaagaaga actggaattt
    12421 taataaatat tatttctctg ggaacgggga ttttttataa tcaatatgac gagttgaaca
    12481 ttatattttt tataagggtt atatgagatt aaagaaatct gccgtaaaga cagatttctt
    12541 taaaagataa ttagagattt gcgacgttat gataaacttt ttgtacatcg tcgtcatctt
    12601 caagggcatc aacaagccct tcaaagattt ctaaatcttc tggggaaagc tcaacttcag
    12661 attgagcaat catttctaat tctgttgttg agaactcagt gattccagct gcttttagag
    12721 ccgcgattcc tttatgaagg tcagtaggtt cagtataaat aacaatgtta ccttcttctt
    12781 cagtcacatc acgaacatca acttcagctt caagtaaaat ttcaaaaata tggtcagggt
    12841 ctgtcccctt aaatacaatc acacccgtat tgtcaaacat atagctgaca gaacctgccg
    12901 ctccgatatt gccgcctttt ttattgaaaa ttgtgcgaac gttagcaatc gtacggttaa
    12961 catttgaagt caatgtctcg gcgataatca ttgagccatt aggaccaaag ccttcataac
    13021 gtccctgcac gaacgtttca tctccgccgc ctttggcttt atcaattgct ttatcaataa
    13081 cgtgctttgg aacttgtgcc tgctttgcac gttcaataac gaattttaaa gatgtgttta
    13141 attctggatc aggttcacct tgtttggcag cagcatagat ttctacaccg aattttgcat
    13201 aaattttaga cgttgcaccg tctttagccg tttttttagc aacaatattg gcccatttac
    13261 gtcccactgg aatgatctcc tcgaaaattt atttacatta caggtaaatc gttaacatgt
    13321 gttaattcgg catattatat cataacagga aaaatctcgt ctattagaaa taaaaatatt
    13381 ctaaagccat aatcttatat tttgttttat aaatactaat gttgttggcg ggatttttta
    13441 taattatgtt tttaaagcat acttgctttt aatatgcgga aatcattaag acgataatga
    13501 aactcgcatt tgcccaagga agcaaacagc ccgcgtttat tgcaggctat ttgccagatg
    13561 tcttacttaa tccttcacat ctatttctga taagaaggtt cagaacttat tatcggaacg
    13621 gcggctcatt aaaggttcgt aatttacgtg aatgcagtcg gtcgccttct gcgcgcagca
    13681 aatcgatcgc ccgaatgcca atttgtaggt gttcggaaat agcgccttca taaaaacggt
    13741 tagcctgacc gggaagttta atctcgccat gcaacggttt atctgaaaca cacagtagtg
    13801 tcccgtatgg cacgcggaaa cgatatcctt gcgcggcaat ggttgcactt tccatatcaa
    13861 ttgctacggc ccggcttaag ttaaaacgaa gtgcagaagc tgagtaacgt aattcccagt
    13921 tcctgtcatc tgtggttacc acagtaccag tacgtagccg ctgtttgact tcctcaccgg
    13981 gcctgccact caccagcttg gtggcgtcat aaagcgcacg ttgcacttca gcaatgctcg
    14041 gaataggaat atcgggcggc agaaccgcat caagaacgtg gtcatcgcgt aaataagcgt
    14101 gtgcaagtac ataatcgcca atggcctgac tttcacgtaa tccgccacag tgaccaatca
    14161 tcaaccagac atccgggcgt agcactgcca gatgatcgca gatggtttta gcatttgacg
    14221 gtcccacgcc aatattcacc agagtaatac cctgaccatc ggcggtaatt aaatgccatg
    14281 ctggcatctg atgttttttc catgcaaggt cggaaatggc ttcttctggc gcttcggttt
    14341 cggcggtgat ccagttcccg ccagcacaag aaagggcaat gtagggacta tcaggatcga
    14401 ggatctggct gcatccccaa cgaacgaatt catccacata acgtgtgtag ttggtaaaca
    14461 agacgaacgg ctgaaaatgt tcaactggcg taccggtata atggcgcaac cgtgcgaggg
    14521 aaaaatcgac gcggcgcgca tcaaaatgcg atagggggga gaattcagtt ggatgataaa
    14581 tgccgtctgc agtttcatcg ccaatttgcg ccagttctgt tgtcgggaag tagcgagtta
    14641 acccagcgct cattgagcga tcaagtgtca attcagagcc atcgatgaca taaggataag
    14701 ggatttcatg ctgcgagggt tgcacagaga tatgcgcacc ataatcctga tacagcaacg
    14761 taagttgttc attaagatac gaacgaaaga gagtagggcg agtaatcgtg gtggtgtagc
    14821 tgcctgcgtg agtaaaacga ccaaatgcgc gcgttttggg gggattggtt gtgctaccgt
    14881 cccaggttac ggtcagtgat ggatagacaa aaagaccttg tttgcgggcg ttttcatcag
    14941 gtaattcgcc acttgtaata tagttgccaa tggcgttgcg taatgcgact acagattgct
    15001 catacagcgc gtcgagttta tccagtgcct gagctggggt cagaccggag cccttattat
    15061 tcatgtgtgt ctcctgttcc atacaattac cgcacatccg atagtatgtc acaggaatgt
    15121 gaaacaaaag tgtaaggcga agatcggatg atcttcgcca gactatcaag cgcgttgatt
    15181 gtctttcatc aacaaagcgg tcattgcgga aatcagacat ccagccagca aataaatggc
    15241 gacgctatgc cagttcccgg caaagtaagt gatgagtgca gcggcaataa aaggtgtaaa
    15301 tccaccgcca accacactgg cgacctgata accgactcca gcaccactat agcgataact
    15361 ggcaccaaac atttcggtaa acatcggttg ttgcacacac accaccatgt catgcgcaat
    15421 gtttgccagc attatggaga agaaaactat ccagaaaata gattgtgctt caagcgccat
    15481 aaagaaagga aatgcgctca acgttccgat taacgcacct gtgatataaa ccctgcggcg
    15541 accaaaacga tcggcaagcc aggcaaaaca gggaattgtc aagcagctta atccacctac
    15601 cagcaaacca atattgagga aaagttcgcg cggtaacccc atattctggg ttgaataatt
    15661 aagtgcaaag gcagtaacga tgtacatcgt cagcaattcg cacagtcgta gcgcaataat
    15721 cttcaggaaa gcaccgggat gtcgtaacag cgcttcgata accgggatgc gttttttcgc
    15781 ggcagcttga taatgttgct gttgttcaaa ttccgcggac tcctccatgc cattgcgcac
    15841 ccacaatgct cccagtacca gtacgatgct aaacaggaaa ggaatgcgcc agccccagct
    15901 taaaaactgt tcgtcagtcg tcatcatact gatcaatgaa accagtccgg ttgaaagcag
    15961 taaacctaca ccgtagccaa cttgtacacc gctactgtaa aaggcttttt tatttttcgg
    16021 tgcactttca acggaaagca acgccgcgcc tccccattcg ccgccgacgg caaatccctg
    16081 aatggcacgc agtgtcacca gcaaaatagg tgcccaccac ccaatggtcg agaatgaagg
    16141 aagaatacca atcaaggctg tcgcgatgcc catcatccag acggtcagca ttaacatgcg
    16201 cttacgtccc agtcggtcgc caaagtgacc gaaaatgaca ccgccgagcg gacggaaaag
    16261 gaatccgacg ccaaaggtgg caaatgcggc gagcgttccc atcgccgggc ttacttgcgg
    16321 gaaaaactcg cgattaaaca ccagtgcggc ggtgatgcca tagagtaaaa aatcatacca
    16381 gtcgacgacg gcaccagcga agctgcctaa cgcagctcgt cgggcgcgac ttaacgaaag
    16441 cgtcccttca tcgggacgag tggagatgag cgtggagtcc ataatgatcc tgtttgtact
    16501 gtctttttat aattagtatg gaaaaggtaa ctcacattgc cgtcgatcta taaacgaacc
    16561 ttatgagact acctcgacag agcgcatctg caaatgattt tatggctgat tagcggcaac
    16621 atgaaaaatt cgatggaaga tgaaagcgca ggagttgctg gtgttgttta aggattaagc
    16681 agaggagcca caggttagag atatgaaaca taaaagtccg catatgcaca aaatggcacc
    16741 ctgatgagtc tgatagactg cattgcatta taaccagtag ggaggcgagg ttaggtatgt
    16801 ttttagcatc attgttgaga cgtattgcat ttagttacta cgattataaa gcttataatt
    16861 tcaatattga aaaaacagac tttgttgtca tccatattcc cgaccagatt ggcgatgcta
    16921 tggccatctt tcctgttatt cgggcgcttg aattgcataa aattaagcat cttttaattg
    16981 ttacgtcgac aattaattta gaagtgttta atgcacttaa acttgaaaag actaaattaa
    17041 cattagtcac aatgactatg caggatcacg caacattaaa agaaataaaa gatttagcaa
    17101 agaacataac acagaaatac ggtacgccgg atctttgtat agaggcgatg cgtaaaaaga
    17161 acctgaaaac gatgatattt atcagtcagt tgaaagcaaa aacgaatttt caggttgttg
    17221 gtttaaccat gaagtgctac tcccctttgt gcaagaacgc gtcccggatg gatcagaatc
    17281 tccgggctcc tgtacccatg acatgggcat ttatgatgcg tgaggcgggt tttccagcag
    17341 tcaggccaat atatgaattg ccactaagtg aggatgtact cgatgaggtg cgcgaggaaa
    17401 tgcgatcgtt aggatcttac attgcgctta atttagaagg tagctcgcag gaacgtacat
    17461 tttcattatc gattgcagaa aatctaatag caaaaattca aagtgaaaca gatataccaa
    17521 tagtgatcgt tcatggacca aaaggggaag ataaagccag ggtattagtg gattgttata
    17581 ataatgtcta ccgtttatcc ttatcaccct cgattaaacg ttcagcagca atcataaaag
    17641 atgcttatat cgcaataact cctgacacct caatattaca tatggcaagt gcctataata
    17701 ctcccgttgt tgcaatttat gctgattaca aaacgcgatg gcccgcaatg gccgatgttt
    17761 cggagtcagt cgtcgttggg caaaaaattg acaatataag tctggatgaa ttcgcaaagg
    17821 cattaaaaag tgttttggcg agaatatgag acagtactta tgcatagtaa tgccgatctg
    17881 ttatgaacgg tggtgtcatc aatactctga ggctggtcaa tcatcacaga aaaccagcct
    17941 ttggatcaaa gctgtactca ccggatacga tcatagtcac atcgaacgcg attcttgcgg
    18001 tgcagcagtt ttatttattc attttctgtg agagaagggc aagctctata gagggaatgt
    18061 aaaaaagagt gctccgaatg gagcactctt taagaaatta taacggaaaa tcacaggttt
    18121 ttataacatg tcgcataagc aaacccaagc ttttcaaata cgctttgatc acctgttgcc
    18181 agggaaacta cgtattgttc gcttgagttt gcgggactag atgaccaata catatttgat
    18241 tgaaaaccag cgtcagtcgt gtaatgcccc atatctcccc attcactgta cagtgacccg
    18301 atgcctcggg aactactgtg gtttaaatag tttgctctgg ggagcgtgta gccattgctg
    18361 ctgcaaaaat tttcagcaag gctgtatatc atgaaagcct cgccggcgtt cacccaccaa
    18421 ctcttcacac ggatttcgta tacatagcta gggcctcctg attttggcgt cgccgtaatc
    18481 ctttcccaat tgctgccgac atttttaaat gtcactttac cggtagcatc aacatcgacc
    18541 caggaggcag agcttgaaaa ctcataatca gccgccgttt ttcctggggc aaagttgtca
    18601 ttattcaact gataatacgc cccggtgaac ccctgcgaag ggaatgtagt tgtcggtagg
    18661 tcagtaccat tgactgatac tgtaccgctc attattttgt cttctgcgcg agtgaattgt
    18721 attgtggtac tcagaccagc ttgatgaaca ccattcaata cagggatcag cgttgcgata
    18781 ccctctccgc cgcttgtaac agtggctttg taatcaccgt tgatatttag actgtaatca
    18841 attgcgctaa tttttatata gggcacgtta gtacctgatt gcacaaattc catcccttca
    18901 gaaactttga tcggattgcc tgatatatcg tgcagaacaa gacgtaattc agcactatcg
    18961 gtatagtccc ctttcagtga tgaccgattg ctcttaagga cggactgcga ggtgtctgcc
    19021 gggccagcca ccagcgttat atctactgtt tgcattccac cagcggtcgt gactgcgctt
    19081 accgtgacgc tacctgtcat cgctcctttc acgcttgttg tcgcgattcc gttttgatcg
    19141 gtcaccgctg ttaatgacgt taatgtggca gagccgcttt ttaaggcgaa gtacacagtg
    19201 agaccttcga tcaggttacc actgccatcc tcaaccgttg cctttaaggt acttaaatca
    19261 ctgttggtgg cggcgatagt cgatggatca gcgataaagc tagcaacatg tgcggtgctt
    19321 gagttgccag tgactttcac ggttgtctgt gtctgtattg ttacgccgtt atggaacgat
    19381 gcagtcaccg tatatgtacc gactttattg ctggtcagaa cgaccggagc ctgacctgaa
    19441 gagttagttc tcacttttcc gccacttaac gtcgcccctt ctggcgttac gctaaagttg
    19501 atgacctgac cctccactgg agtgccattt gcagaggtta gcgttgccgt cagagtagtg
    19561 cctgtagggg catagacacc gataagcgga ctggacgccg tgagtgtcag tttttcatca
    19621 atagcctcca gttgtttctc aagtgaggct ccattcgcca gggatgcttt caccatatac
    19681 gaaccgtttc tttcgggcgt catggagacc tcggctatac catgcgtatc agtagagaca
    19741 atattttggc tgatggtcat gtgctctggt ggctctgcac tgaatgttac agattcatta
    19801 agaatcgggt tgccaaactg gtcgttgacg tgagctttaa ctgctacgtc ttgtgcgacc
    19861 attagctgac cttcaggtat ctccagactg gtaatcgttg cagaattaat atctgctttt
    19921 gcattcagta atcgcgatat gacttcagta ggtttatctg ccagagaggc tgaaactgtt
    19981 ttcagtccga cttcggtgct tgtcacaaga atttcagcga aaccctgatc atccgtttca
    20041 acgctggtgc tgcttagcgt gacggaggtt ccgcggaaat taacttttat gccttctacc
    20101 gggttgccgt tagcgtcagt gacgcttgca gtcatggtcg cgccctcggt cgtgctgacg
    20161 acgaatgagt atacagaggt taaggaggct agtgttgcgg taccagcatc ggcaatcaaa
    20221 gtcacctgtt tcgtatcact gacaccataa ttgttcacgc taactgtcac ggggtaggtg
    20281 ccggattttg taccgctcag tgtaacttca gccttgccgt tgatatcagt aatggcggaa
    20341 ccgccttgtc cgagagtgaa gcttgcgctc acgtctgccg gtagcgtgaa tgtcaccgcc
    20401 tcattggcta acgggttgcc gtttccatca gtcactgttg cctgaagtgt ggtcatccca
    20461 acgttattgg cgataaagtt gtcctcggta acgttaagat taacctgcgc actgagcgta
    20521 tccgcaataa agttcaccac caactgctca ctcttaccgc cagtcatcga tgctgtaaca
    20581 gtatgagcgc ctgcttttgt acctttcagc gtgactttcg ctttgccttc agcatcagta
    20641 atcgctttac cgccatcgct cagcgtgaag ttcgccttca catcttccgg cagagtaaat
    20701 gttacttcag tgttggctat cgcattgccc tctgtatcag cgactgttgc tgttagtgtc
    20761 gtgagatcgt tattgtcggc aatcaacgga tccttcgagg ctgccagcgt gatttcggca
    20821 ttcgcgacgt tcggcacata ggtcactgtt tgttgcatcg aatcgccatt ttcgagggtt
    20881 gccgtcactt gataaacccc ggcttttgta gcggtaaagc ttgcgtaaaa attgccgtcg
    20941 tggttggtag tatatatagc gttattactg ggggtcacgc cttcacttgg tgatactctg
    21001 agggttacct cctgctgggg tacaccgttg ccgtaattat ccttcacctc aatatacaga
    21061 ttggtagtgt cgcctgcgga gactgtatca aaaagtgcct gtagcaaggt gagatgtgcc
    21121 gtagacgcat cagcgttgac attaattgat gtgctaagcg tggagctacc attttccaga
    21181 ctggcctcaa cggtatccgg tctcgctcct gattctatcg aggagcgggt attggtataa
    21241 gtgacggtag ccttaccctg ttcgttcgtt acggcttggc cgccattcgt catttcggca
    21301 gagtttgttc tgctggtgaa gttcacagtc acacctttca ccggaaagcc attattatca
    21361 acgactgtgg cggtgatgac gctgccggag ctgttctgcg gggtaccggc gattatgctg
    21421 tctgggacag gcgtcaactc gataattttt gccgctgctg tgtcgccaat aaaatgcaca
    21481 gttttgttgt cgctggcacc attattagcc agtgatgcag tgaccgtctg ctcaccaaag
    21541 gcaacgcctg cgagagtggc ctgcgcgatg ccagactcat tggtattact tactcccggg
    21601 gtcagggtga gtcctgaaga ggctgagctg aatgttaccg gaagattatt cacctcattg
    21661 tcgaactgat ctttaaccgt tgcagttagc gttgcgttat cgacgccatt acctgtgatc
    21721 tcatcttttg acatctgcag gacaacctga gccgaggttt tgtccgccac aaatgttacc
    21781 ggctgcgaat cactggtatt gttattaccc agcgttgcgg taaccgtatg tgtacccgct
    21841 tttttacctt tgagcgtgac atgcgcttcc ccattggctt gggtgatggc aatgccgtta
    21901 ttttcgaggg taaagtttgc cgccacgccc tgtggcatgg tgaagttcac cgttattccc
    21961 gccaccggat gattcgacgg atctttcact gttgctgtca gagttgtctc atccacgcca
    22021 ttcccaatga tttccgcttt cgatgtttgc agaacgacaa ccgctctgtc tttatccgcc
    22081 acaaacgtca ttggctgtgt atcgctgaca ttgctgttag ccagacgagc cgtgatcata
    22141 tgcgtgcccg ctaacgtgcc ggtcagggag gcgattgcag tcccgttgct atccgtcatg
    22201 ccttttccgc tgttgctaat cgagaaccga cttgcgacgt cgtttggcac agagaaggtg
    22261 atttctttat cttttaatgg gttgccattt ttatcctgca aggttgcagt catatgtagc
    22321 ggagctgtgt tggtgacggt gatatcacct gaaggcacac tgagggtcag ggcagcagta
    22381 ctttgatcac cgataaaaat cacctgttga ttagcctgag aaccagagct cacagaggcc
    22441 gtaaccctat aatcaccatt tttcaaactg gtcagcgtag ctgtggcgat cccgtcgtgg
    22501 ctattcactt ctgtttggct cagtttcgcc tctgctgaat taacattgaa agtgaccttg
    22561 acgtcattga gcaggttgcc ttttgcatcc cggactgtcg cggtcattgt ggcgctatca
    22621 ttgccgtcag cgaccacttc atttttcgac ttctgcagct caacctgcgc ggtactcgag
    22681 tcgccgacaa aactgacgat taacgtttgt ttcacgccat tttcaagggt gacttcaacc
    22741 gtgttgtctt cctgcttact acttttcaga tcaaaagtcg ccagaccatt aacatccgtt
    22801 tttgcggtgt tttgattgtt gaaggaagtt gccgatccgc ttaataccgc aaacgtgacg
    22861 gtatgatcat tgattgggtt gcttccttca tcagcgacat tgaccgagac ggtgtttgct
    22921 gcattctcat tggcgagcac accattattg ctggcagata atgtcgcaat ttttgctgac
    22981 tgcgggttgg cgtcgatgat aaaaccagcg gtatgcaaat cttcattcca gttttgcatt
    23041 aatagcttcg cagtaagccc actgcctttg gtataggcgg tataggttgc cttatagacg
    23101 ccatctgcgg tttctttcca gtctgtagtg acaccaggtt tcacgttgtc gatgctgact
    23161 gcgttattca gttgctgttt ttgctcctta acaggtttgt cattttcatc tctcagttct
    23221 accgtcacct cgataggatt gccggagaga taacggtctt tatcaatttt aattgacgag
    23281 tgagttcggg atgacgaaat agaaatgata ttcaccacgg cgggggcttt agccgcatcc
    23341 acaccattca gctgtggcat cagcgtcagc gtgccagaca tcgctcctgt ggtcaggatc
    23401 tgggtatagc ttccgtcacc attatctttc cattcagaaa gggtgatgtc ctgaactcct
    23461 tcgtgacgcg tcgaaagcac cagcccgata acaggattac ctgctgcatc atgagcaata
    23521 aaagtcagtg tggcggttga atgggaatcc gcgctcaatg tttgggtact taacgatacc
    23581 gaggaatctt tctggcttag cgtaggtgcc tgaacgacca ccatgctttg ttcacgattc
    23641 gacaaattgc ctttgacatc ttcagcggtg acttcaatcg gccaggtgtt atcggtttct
    23701 ggcgtactgg tgaaccggta agccggcagg gtaaccagaa tatctttacc cgttgtgacc
    23761 actttgccac cggcagcttc cagagcggtg gcttcgacgt tatagccttt cagggcatat
    23821 ttggtttgta gcgacgaaac caatgatttc acttctcctg acttccctgt cacggggtct
    23881 gtcagggtca ggcgaaccag ttcttttttg cggtattcca gaacgatgtt gttgttgcga
    23941 tccaccagat cataacggct gcctgcaagg ctacgccgtg cagcgacttc gttcgggtca
    24001 agctgtttct gcattgcgct gccaggttgc caggtaaaat cgacggcaaa acgggtgtca
    24061 ttttcgccct gtttaccctg gcgttgctcc gcgctgaagg tcatcagcgg gaagggggta
    24121 tagttaagtc cagcggttat ggtatgagga ttactttgcc gatcgtcttt atcgaacagg
    24181 gccacttcat cgccataata ctgttcatag accagtttac cgccaaggtg cggccaggcg
    24241 ggtagccagc cttctgcgcg tacatcccag ccattggccg ggcgggcttc ataatcgttg
    24301 tccagctcag gtgcgctgcg ccagttggtc agtggcaaat agccgttact gcttaatttt
    24361 agatagtcgc gccagtactc cgcgccaatg ccggcgcggg agtggtaacg gctaagatcg
    24421 tggtcgaaaa agaagttgat gcccgacatc catgtgggag tgaaatgacg ccaacctaag
    24481 ccgttgttaa tctgcgtacg ctcgtcagta cgatggagag tatgctgact gaaaaagaga
    24541 ttatcaggcg tttcatacca cggatggaga aaatcgaact gggagttctt caggctaaaa
    24601 tcttcatcca cgcccagcgt gattcttgcg gtaccgaagc ggcttaacca gtctgtcatt
    24661 gcgcctgaag cctgagaaga ggcccatcca cgcgccatat ttgccgcttg ctcgctgttc
    24721 atatcttcgg cgagcagaga cccgattggc tgtgaagtac tggctatctg ttgctcaagg
    24781 ttgccactgc tattacccgg tggcggggtt aaattatttt cactaacttg tgccgggaca
    24841 tccagttcat caccctggcg gacattatca aaacctcgag caaacgtacg aaactggttg
    24901 agtttgcgta actcagccac cgaaataccg aaacgttcgg caacgctttg ggccgattcc
    24961 agcgctccaa gggtgtaggg caccgtattg gcatttgcaa tggcaatttg tgcaggaact
    25021 ggttgttggg ttgcggtgtt taccacacct tgtgctgccg cagccatagg gaacgcaagt
    25081 tgagttatca ggcagatacc cgcagttaag cggcgtagtt taattcccat cccgcataag
    25141 atttgtcggt catttatttc ttctccactt ctcttcttcg tagccatgct taccttccct
    25201 gaacgagcta acataaatgc gccctttcta gcacagcgaa aaaatgtaaa tcctgatagc
    25261 aatatgattg aaaattaata acttttcgtt aggcagtttt gggtgtgagt tgcaagaggg
    25321 gagactactg aataactcaa gttttataat cgaggggaaa atggtgatgg cgttcatagc
    25381 aaaacgccct caaccataaa ggtcgagggc gcttaagatg ttaaaaaacc gctatccgtt
    25441 aaaaagcaat gttcaactaa ggtcagtgac attgcgctaa aaaagcgaat ttttagaatt
    25501 tggctcctct gactggactc gaaccagtga catacggatt aacagtccgc cgttctaccg
    25561 actgaactac agaggaatcg tgtgaacggg gcgcatatta cttagcggta ccttgtctgt
    25621 caacactaaa tttcataagt catttcaatt ggttaattaa tctgcaaagt tgttaattaa
    25681 tgaacattcg tcgccgaaaa cgagtctgta tcattagtgc gatgcagtct ctgcaaagga
    25741 tcttgttgat aaaattggca gaaacgttgc cacaatgaag ggaaacgagg agcaaaaagt
    25801 tctggggcgc taaagaaata ttcagaaagt acggcaaaac attcagcagg atcactggca
    25861 gcataagcat caatgctcgc cgcattgtcg ccaaccaatt cgatttcttc ctgaatgttg
    25921 ttcattgcag catgaagatc gtgttcccag ccagcaacct cacgcaacgg aataaaggga
    25981 actccgctgg cgcgatcgcc gttacgggtg tccagcttat gagcgacttc atgaataatc
    26041 aggttaaaac cagaagcatc aaaagaatct tgtatatcca accagttcaa aacgataggc
    26101 ccttgctgcc agctctgacc tgactgaaca atacgttggt tatgcaccag accgatatcg
    26161 tcttcccatt catcatcgac cacaaatggc gcaggataaa ttaagacttc atgaaaacca
    26221 tccagccatt ccagtcctaa ctccagaacg ggtaggcaaa atagaagtgc tatccggcag
    26281 cttcttaatg aatccagttc aaagccctgt aaaggaacaa gccgcttttg ctgtaaaaaa
    26341 cgttcggcaa gagtgactaa tttgctttgt tcctgttccg tcagacacgt taaaaggggg
    26401 atcgatagtg cttcctgcca gggaagggca gtttgatgtg ctgattcttg tactttccag
    26461 ggccacttaa tcatcgtttt gctcgcaaac tcgtcacttg aacaaaattg cacggacagg
    26521 gactgttaaa atgccaaatt tcctggcatc atggcaacca tctgaacgga gagatgccgg
    26581 agcggctgaa cggaccggtc tcgaaaaccg gagtaggggc aactctaccg ggggttcaaa
    26641 tccccctctc tccgccactt tatcaatgac ttatctcctg acttcccgcc ttgcttttcc
    26701 taaacagaac aatcgtagaa tattcttgaa gggttagatc gtcactgttt tctgttcgat
    26761 actgtgacat tcagcacttg atccgctatg gatctgacag gaagctttcg agcgaaaatg
    26821 tgcagttatt cagtcgtttt cttatcggtc accattattc ttttagacat tcatcctatg
    26881 aaactgccgc aaagttggtg gtgggaactg aagttgcgta gagaaggggt caataattga
    26941 gtcaaaaaag gaacgcggcc gtcagattaa agccgcggga caaagtccat aatttccaag
    27001 agttgttgaa tttcccagct tttttgaaag cctttgtatc atactaacaa gtaaaaaagg
    27061 tttaaatacc aaaattaacc tgtatttttg ataagtctta ccattgtacg atttacacag
    27121 tttctgctcc tccttgttga tagtggaaag ggtgcattgt gcacttgcgt aaacatttat
    27181 gacgcgttga tgtccatggt tcacatttcc ttgtcagggg atcgacttat ttgcggcgag
    27241 gcataattcg taacagagta ttatctttct gaatgtagtg atgaaagagt gctgcgccag
    27301 cgtgagctgc gattaaaaag taacctatat tcgcccaggt ttcgtgaata ttttttatca
    27361 gtgctttaat ttcgctgttt ggggtaacaa agggagacac attgaaacca aggaaactcc
    27421 acgattttcc actgtaagcc atcaaagcaa tccccagcag aggaagtgca aggaaggtta
    27481 tataaagcat gatgtgcatt agcgaagccg ctttcatttg ccaggcaggt ggcggtggta
    27541 caatagaagg atcactataa cggtgtttta taatcaggcg tgaaaacatt aacacccaaa
    27601 cgaatatacc cgcattgtaa tgtgtttctc gcatcagcag ataagtacta ctacctttag
    27661 gaaaccagcc acggagttcc atcgcggcat aggttattgc aacgaaaagc agcgttatcc
    27721 aatgtaaata aatttgagtt tttgagaatc ggttcatttt tgtagctcct gtaattttta
    27781 cagtcaagct atcccacgaa ccttaaaaat tccttataca gatgtggttt taagaatttg
    27841 ttatttaaaa aattgatagt gcacattgta accagccaat ataagcaatg gcgtgagcac
    27901 aggtatcttt ctcagttggc cgtattgtgt atggaatccg ttattggtta actcaatgcg
    27961 ggttagctta ttaatataca gacacgtcat agtgatgagc atgtctgtag ctagcttgcg
    28021 ttcagtggcg tttgtggatg gaccagtggc agttatgatg acttactaaa gcggcatcga
    28081 ggcgttatca tgagaatact caatgagaca tcatttcctc gaccacttct tcgctactca
    28141 actgatatgg ataatacgtt ggccagtttt ccatttcatt taataaagat tgctgtgaat
    28201 cattacccat aaaaatgtga aaatgagatg attttcgtgg tgcaattata tgatcgctaa
    28261 attgtatata tttaggggct ttgctttcag gatctttaca ttcgaataag taacgaacgc
    28321 ctttcttgcc tgatttatag gtgagtattt tgtatccatc gtactcatat ttacaggatg
    28381 ttgtttcatt atttctatgg aattcaacaa tgccgtcctc aatgccaatc atctcaatat
    28441 ctgttgcata acctttgtga taataatctt taatttcagc aaatgtttta gttttatctg
    28501 catccgcttt cttctgaaag acggggtcaa gtttgccact ttgcagtaaa ggataaacgg
    28561 attgccagac tccatcccag tcactgagcg ttcggttttg tacattggca tcatcaaaaa
    28621 caccattagc agctttttgt tcgacctctg ttaagggttt gccgtgtgag tgatgaccat
    28681 gcgaaaaggc aggagcgcta acaataaaga cacctaaagc aacagccagt ttgtgaagac
    28741 gaatcgccaa aacagtttcc tccagagtca ttgtatatga tgtgtaatgt tatattgtaa
    28801 catgtgacaa aatgcaataa ctcttaggag atgagagcat agttttctca ggtatactta
    28861 tctatcttat tgaaataaaa tgaattatgt tgataatttg aggttgatga caaacgaaac
    28921 ataacctgat gtgatctgcg taggccggat agggcgctcg cgccgcatcc ggcagggata
    28981 acgcgtactt tgtcggcaat atgaagttat acagataatt tgttgtgaac ctgcttgcgt
    29041 aaccggttaa aaagggaacg caacttctta taccgtaagg ctaaaagcag tacagccagc
    29101 ccagcgtaga tgagcggctg cggtgaaata atcttcactg accacagata atgtattggg
    29161 gccaggatcg cgacaagata gacgaaatta tgcaactgtt gccagtgctt gccgagtttt
    29221 cgctgcatcg cctgggttga agtaaacgct aaagcaagca aaataaccca gctgataata
    29281 cctaacgtta aataaggccg ggtaattaac tccttaccta ataacgccag attattcacg
    29341 cccagctcca gcaatgcgta actggttaaa tgcagtgtcg cccaggcaaa gcaccataag
    29401 cctaacaggc ggcgagtgcg tatcaataac ggctgtttta cgtagcgtgc cagaggtgtg
    29461 attaacaagg tcgccagcaa aaatttcagt gcagtgcgac cggtaaaatg ctgaatatct
    29521 ttcaccggat cggcacccag tccaccgtga ttgatcgccc agaccagcca gagaaacggc
    29581 aacaatccgg caagatgcag gctaactttc agccatgtca cctgttttgc tgtcagacgc
    29641 attcagaaat tctcccgcaa atccaggcca cgatacagcg atgccacctg gtcggcgtaa
    29701 ccattaaaca gtagcgttgg ctggcgctga acatcgagga tgccgcctga accaataaat
    29761 cgttcggtag cctgtgacca gcgcgggtga tcaacatgcg gattaacgtt ggcgtaaaaa
    29821 ccgtattcgt caggcgctgc cagattccag gtggttggcg gacgctcgcg ggtcagctta
    29881 atactgacta tcgatttaat ccctttaaag ccatatttcc acggcacaat cagtcgtacc
    29941 ggcgcgccat tttgtggcgg taacgccttg ccataaacac ccacggtcat cagtgtgagc
    30001 ggatgcattg cttcgtcgag acgcaatcct tcgacataag gatatttcag cccgccgccg
    30061 ataaagcggt cctgctggcc aggcatctgt tccggtgcat aaattgtttc gaaagcgaca
    30121 tacttcgcat tgctggtggg ttcggcaagc gccagcaatt tgtgcagcgg aaaaccaatc
    30181 cacggcacca ccatcgacca cgcttccacg cagcgcatac gataaatacg ctcttccagc
    30241 gggaagcgac gggttaaatc atcgtgatcg agggtcaatg gttttgccac ttcgccgctg
    30301 attttcagtg tccatggatc ggttttcagg ctaccagcat tagcggcggg atcggcttta
    30361 tccagcccga attcatagaa gttgttataa ccggagactt tatctactgg cgtcagtggc
    30421 aggttatttt gccaggcggc aggcttgctg aactccagcg gttttccggc gggggcgggc
    30481 gggcgatcgt tccctttaaa ccagctaagc agatcggcat gcgcagcgtg aggcaaagaa
    30541 agtgcagctg cgctgatgcc cagtgctttt aacacctgtc gacgcttcat aaagaatacc
    30601 gactcggccg taacatctga ttcttttaaa aattgattct ttttcataag ccatcacact
    30661 tttttgtcgt tagccagcaa gagccgttta ctgttaatct gtcagggagt gtcctcccta
    30721 acagtagcag agatctgctt ttacatattt atgcattaac tgccacgata ggttgaatac
    30781 ccatattggc taagtaataa aggcacatga taatgctcgt tcactttatt aataagaaat
    30841 tcaacgggga tctcagggaa gaaactttca agattttgtt tcttgaaata gtccccggtt
    30901 ttaaatacga cacggtaatc gccagtagtc gcagtttgct cgggccacag tgccttaatt
    30961 cgtccatcct tatctgtttt ggcggtatta agttgtaacc agccgttatc cgccttcttt
    31021 tcaagagtga ctgtcacgtc agcagcgggt tttccggttt gctggttcaa aatgtgcacg
    31081 ctaagaatgt tttgttgtgc ggcataaacc aaagaaggta atgaaaatgc tgccgttact
    31141 acggagagta ctaaataacg ctttaacatg tttatatcct tgtcatctga atgagttttg
    31201 aggatagcga aaaaacgtcc agggtaacct tacagcagca ttacaatttt gtaatgaacc
    31261 aggctgtttc tataacatat gatttatggc atattatttt catgaagatt ttacttattg
    31321 aagataatca aaggacccag gaatgggtaa cacaggggct ttccgaagcg ggttatgtca
    31381 ttgatgccgt ttctgatggc agagatgggc tttatcttgc gttgaaggat gattatgcat
    31441 tgatcattct ggatattatg cttccgggta tggatggctg gcagatctta caaacgttaa
    31501 gaacagcaaa gcaaacccct gttatttgcc ttactgcaag ggattctgtc gatgacagag
    31561 tcagagggct ggacagtgga gcaaatgatt atctggtaaa acctttttca ttttctgagt
    31621 tgctggcaag ggttcgggca caattaaggc aacatcacgc tttgaattca acattagaaa
    31681 tcagtggttt aagaatggac tctgttagtc aaagtgtgag cagggacaat atcagtatta
    31741 cactgacgcg caaggagttt cagttacttt ggctactggc ctccagagct ggcgaaatta
    31801 tacccagaac ggttattgcg agtgaaattt ggggaatcaa ctttgatagt gataccaata
    31861 cggtggatgt cgccattcgc aggctccgcg caaaagttga tgatcctttt cctgaaaagc
    31921 taatcgccac aatccggggg atgggctatt cattcgtagc ggtaaaaaaa taatgaaaag
    31981 attatctata accgtccgtt taaccttgct ttttatattg ctgctgtctg ttgctggcgc
    32041 tggaattgta tggactctct ataatggcct ggcaagtgag ttgaaatggc gcgatgatac
    32101 aacactcatt aaccggacag cgcagatcaa gcagttgtta attgatgggg taaatccaga
    32161 tacgttacct gtgtacttta accggatgat ggatgttagt caggatatct tgatcattca
    32221 tggtgatggc atcaataaaa ttgttaaccg gacgaatgtc agtgatgaca tgttaaataa
    32281 catacctgct agtgagacaa tcagcgcagc tggcatttac agaagcatta ttaatgatac
    32341 agagatagat gctttacgaa ttaatattga tgaagtttcg ccatcattaa cggttactgt
    32401 ggctaaattg gcttcagcca gacataacat gcttgaacag tataaaatta atagcattat
    32461 aatttgcatt gtcgccattg tactttgctc agtattaagt ccgctgttaa tcagaacggg
    32521 attacgagag atcaaaaagt tgagtggtgt aacggaagcg ctgaattata acgatagccg
    32581 ggagcctgtt gaggttagcg cattaccgag agaactaaaa cctcttgggc aggcgttgaa
    32641 taaaatgcat caagccttag tcaaagattt tgaacgccta agtcaatttg ctgacgatct
    32701 cgctcatgaa cttagaacgc ccattaatgc attactgggt cagaatcagg ttacgctcag
    32761 tcaaaccaga agtatcgctg aatatcaaaa aacaattgcc ggtaacattg aagagctgga
    32821 aaatatttcg cggttaacag agaacatact gtttcttgcc cgggcagata aaaacaatgt
    32881 tttggtgaaa ctggactcgc tttctctcaa taaggaagtc gaaaatttgt tggattatct
    32941 tgaatacctt tcagacgaga aagagatttg ctttaaggtc gagtgcaatc agcaaatctt
    33001 tgcggataaa attttactgc aacgaatgtt atcgaatctt attgttaatg ccattagata
    33061 ttcaccagaa aaatcgcata ttcatataac cagttttctt gataccaacg gctatcttaa
    33121 tattgatgtc gccagtcctg gaacgaaaat tcatgagcct gaaaaactct tccgtagatt
    33181 ttggcgggga gataattcgc gtcattccgt aggtcaggga ctaggccttt ctttagtcaa
    33241 agcgattgcc gaattacatg ggggaagtgc tacgtatcac tatctcaata agcataatgt
    33301 gttccggatt acgttaccgc aaagaaatta acataaggct tcagtacgcc atgaattaac
    33361 cgtgaaaaaa cgcagccacg tcgtgagtac taacgcggct gcgattgatt agcgcttaca
    33421 ttcaaacgta acagggatta acccgcgtaa gctgccagca tttcctgcgc cgccagttta
    33481 cccaacgcat tcgctgcaaa aggactgtcg ccggtgagaa ctttacggtc cttatgtact
    33541 cgcccggtga tgtcgtcatt aataatgttc atgcccattt tcttcagctc ttcgccgaag
    33601 taccaggtga gatgacccgg catatagcct atctctggcg tttgtttgtc tgcggcgtct
    33661 gggaatgcgc aaatggaata accattcagt gggttatcgc cgtggcgaag cgccagaaaa
    33721 gccgccgggc cgtggcaaag ggagataaca aaacggtcat ttttgattgc ccactgcaaa
    33781 gcagcagcta cgtcctggct ttctggtaaa ccaataagtg cgccatgacc accaggaaca
    33841 aagattgctg catattcgct atcagcgttg aggctggcga caacatccgc gagtttcttc
    33901 ggattgcgga acaacgattt atgttgctca aagaatggca ttactttttc atctttgtgc
    33961 ggcatagccc agtattcaaa cttggtcatc agaccggaaa tggtcgccac ttcgaattcg
    34021 aaacctgcag catggagatg atacaacggc agcaacgttt caatcggatg gttaccggtc
    34081 gagaacagtt ttccgttatc ggttggcaaa taacgttcgt ccgcggcgat caccagaatt
    34141 ttatgtttac cgcgatacgg ttttggatag tccacgccat caagatcaga gacaggactg
    34201 gtatattggc taagcgaata ttctgaaggg aagaatgcat tatcttcagc aatatcgacc
    34261 tgcggatttt tacttgtttg aacagtcata gtgtattcct tattgttgct tagttagggt
    34321 agtcactata tttgcgactg agctggtcgc tatttttaac caggctggaa aattgagtca
    34381 atatatcgaa agcggtagtc ggcgggtgta agtatgtaat gatgatgctg cactggtgtt
    34441 gaatattatt cacgccaaat tttccttatt gagcaacgtc gtcgcaagtg gcctcaatga
    34501 ttttagacca ttatcagagg tacggggcgg cggggagaga tttagtgatg tggaatattg
    34561 tggtgctgat gaatatttat atcccggatt ggtagagaaa aagcttattt catgcactat
    34621 ttttgcaaat atttctatat gggaggcggt tttgttgcag attaattcat aaagaaatgc
    34681 atttgggtgt tggctggaag agtcgctggt agatgttatt gtgccagcag gggcagggta
    34741 aggtgctgta tcgatatgct ttggcaattg cgcgtgccgc agagggagat aaaatgccaa
    34801 taaaaaagcc tgagttcgat gagggacgaa ctcaggctag tgttaagttt tacagtaaca
    34861 cgccgcggcg cgttggctga ttagaactgg tagactaagc ccacaccaac aacgtcatct
    34921 gttgcgatgc cattgttgtc atagaaatcg tcatctttat ccaacaagtt tattttatag
    34981 tcaacatatg ttgagaagtt cttattgaaa tagtaggtcg ctccaatttc aacatagttg
    35041 accagatcct gatcaacata ctcttgatga ccattgttgt tatagtcgtt accgccgagg
    35101 tctttgcctt ttgaataaac atatgcaaga gatgggcgca gaccaaaatc gaactgatac
    35161 tgtgctacag cttcaaagtt ctgggtttta tttgcgatac ccacattacc atatggtgtc
    35221 atattgcggg tttcagcata catcattgca agataaacat tgttggcgtc gtattttgca
    35281 ccaaatgtcc atgcttcagc tgtatcacca ccagcaaatt tgctgtattc attgtagtga
    35341 tacccgccaa gagctacttg agaattggta cggtcagagt ttgagtaagc ggcacctaag
    35401 ctcaaaccga agtcaaagtc atatgaagtg gaaagaccaa aaccatcacc gttctggaag
    35461 cgaacgtctt cgtgaccatt tttagtacct tcttgatctt ctcctgcatt gctgccttcg
    35521 ttgttacctt gatactgcaa tgcaaagttc agaccttcca ccagaccgaa gaaatcacta
    35581 ttgcggtaag tagctacccc atttgcacgg cctgccatga agttatcagt ccaggtatag
    35641 gagtcgccgc cgaatacagg aagcatatct gtccaggctt caacgtcgta aatcacacca
    35701 tagttacgac catagtcaaa ggagcccatc tcgccgaact tcagacctgc aaaagcaagt
    35761 ttgacataag tattatcaga accttcagtg tcgctagcct ttgttttggt cagtgcttga
    35821 ccataaccag ttaactgatc tgtaatttgg gtctcacctt tgaagcctaa ctcgatgata
    35881 gtttcatcgc cgtcatcgcc agggttatcg gaaaaagtat gacgagcatc aacttttccg
    35941 tacagatcca gtttattgcc atttttatta taaatttcag ccgcatttgc tgcgccagca
    36001 actaataacg ccgggaccag cattgccaga acttttcttt tcattatgta ttcccttgtg
    36061 attataatct tcatgaatat atcaataagt gccgttatcc aaaaaaaaag cacatttgga
    36121 tactattcta tgaagttcat tttattttaa agattacatg taacaaatgt atttaaaaga
    36181 tttcaaaatg tttctaatct atttaatata attcaggatt atgaattcta aatataaata
    36241 ttcatgaaaa ttataaatgt atatcaatat gttatgagtt gagcggaagc atagggggta
    36301 tcaatgattg ttgtgccaaa cggctggtgt gatcacgcgc acattaatga aggcataaaa
    36361 aaaagccggc atcatgccgg cccaaaaaat ataaacaaga cgataaaagg ctaataacgg
    36421 aggcatcatg acacagaaaa ataaaaaggt ataattgtat tcagtgctaa tatattgatt
    36481 aaatgaatta ataaagccga tctcaaagat aaataaaatc gctcaaaacg atttttcctg
    36541 tattcactgc cgttgcgcaa aaattatcta tttgttcaaa aaatgagtga gtcttgactg
    36601 gctcatccaa tgtggaaaaa tgtgactttt atcacataat agtactaagt ctgaattttc
    36661 cgggttatct caaaatggaa tacggttcga caaagatgga agagagactc tctcgcagcc
    36721 ctggtggcaa actggctttg tgggctttct atacatggtg tggctatttc gtctgggcaa
    36781 tggcgcgtta catctgggtg atgagccgga tccccgatgc cccggtgtcg ggatttgaaa
    36841 gcgatttagg ctctaccgcg ggaaagtggt taggggcact ggtcggattt ttatttatgg
    36901 ctctggtcgg ggcattgtta ggcagcattg cctggtacac ccgacctcgt cccgcacgta
    36961 gcagacgcta tgaataaaaa ctgctaaggt ggcgactctt tggcaacgga gcgcgtatgg
    37021 acttacaaca ctggcaggca cagttcgaaa actggttgaa aaaccatcat cag
//
LOCUS       DAMZFT010000033        36386 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.033, whole genome shotgun sequence.
ACCESSION   DAMZFT010000033 DAMZFT010000000
VERSION     DAMZFT010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 36386)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36386)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36386
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.033"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..797
                     /locus_tag="PN977_001788"
     CDS             <1..797
                     /locus_tag="PN977_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sugar phosphorylase"
                     /protein_id="HDI6041824.1"
                     /translation="FSLPPLVLHAVQKQNVEALCAWAQNLTLPSSNTTWFNFLASHDG
                     IGLNPLRGLLPESEILALVEALQQEGALVNWKNNPDGTRSPYEMNVTYMDALNRRESS
                     DEERCARFILAHAILLSFPGVPAIYIQSILGSRNDYAGVEKLGYNRAINRKKYYSKEI
                     TTELNNKTTLRHAVYHELSRLIKIRRSHNEFHPDNDFTIDTVNSSVMCIQRSNADGNC
                     LTGLFNVSENIQHINITDLHGRDLISEVDIVGNEITLRPWQVMWIK"
     gene            811..2103
                     /locus_tag="PN977_001789"
     CDS             811..2103
                     /locus_tag="PN977_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HDI6041825.1"
                     /translation="MIKSKIVLLSALVSCALISGCKEENKTNVSIEFMHSSVEQERQA
                     VISQLIARFEKENPGITVKQVPVEEDAYNTKVITLSRSGSLPEVIETSHDYAKVMDKE
                     QLIDRLAVATVISNVGEGAFYDGVLRIVRTEDGSAWTGVPVSAWIGGIWYRKDVLAKA
                     GLEEPKNWQQLLDVAQKLNDPANKKYGIALPTAESVLTEQSFSQFALSNQANVFNAEG
                     KITLDTPEMMQALTYYRDLAANTMPGSNDIMEVKDAFMNGTAPMAIYSTYILPAVIKE
                     GDPKNVGFVVPTEKNSAVYGMLTSLTITAGQKTEETEAAEKFVTFMEQADNIADWVMM
                     SPGAALPVNKAVVTTATWKDNDVIKALGELPNQLISELPNIQVFGAVGDKNFTRMGDV
                     TGSGVVSSMVHNVTVGKADLSTTLQTSQKKLDELIEQH"
     gene            2124..3005
                     /locus_tag="PN977_001790"
     CDS             2124..3005
                     /locus_tag="PN977_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDI6041826.1"
                     /translation="MNRLFSGRSDMPFALLLLAPSLLLLGGLVAWPMVSNIEISFLRL
                     PLNPNIESTFVGVSNYVRILSDPGFWHSLWMTVWYTALVVAGSTVLGLAVAMFFNREF
                     RLRKTARSLVILSYVTPSISLVFAWKYMFNNGYGIVNYLGVDLLHLYEQAPLWFDNPG
                     SSFVLVVLFAIWRYFPYAFISFLAILQTIDKSLYEAAEMDGANAWQRFRIVTLPAIMP
                     VLATVVTLRTIWMFYMFADVYLLTTKVDILGVYLYKTAFAFNDLGKAAAISVVLFIII
                     FAVILLTRKRVNLNGNK"
     gene            2992..3834
                     /locus_tag="PN977_001791"
     CDS             2992..3834
                     /locus_tag="PN977_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HDI6041827.1"
                     /translation="MATNKRTLSRIGFYCGLALFLIITLFPFFVMLMTSFKSAKEAIS
                     LHPTLLPQQWTLEHYVDIFNPVIFPFVDYFRNSMVVSVVSSVIAVFLGILGAYALSRL
                     RFKGRMTINASFYTVYMFSGILLVVPLFKIITALGIYDTEMALIITMVTQTLPTAVFM
                     LKSYFDTIPDEIEEAAMMDGLNRLQIIFRITVPLAMSGLISVFVYCFMVAWNDYLFAS
                     IFLSSASNFTLPVGLNALFSTPDYIWGRMMAASLVTALPVVIMYALSERFIKSGLTAG
                     GVKG"
     gene            3865..4917
                     /locus_tag="PN977_001792"
     CDS             3865..4917
                     /locus_tag="PN977_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HDI6041828.1"
                     /translation="MKKLVATAPRVAALVEYEDRAILANEVKIRVRFGAPKHGTEVVD
                     FRAASPFIDEDFNGEWQMFTPRPADAPRGIEFGKFQLGNMVVGDIIECGSDVTDYAVG
                     DSVCGYGPLSETVIINAVNNYKLRKMPQGSSWKNAVCYDPAQFAMSGVRDANVRVGDF
                     VVVVGLGAIGQIAIQLAKRAGASVVIGVDPIAHRCEIARRHGADFCLNPIGTDVGKEI
                     KTLTGKQGADVIIETSGYADALQSALRGLSYGGTISYVAFAKPFAEGFNLGREAHFNN
                     AKIVFSRACSEPNPDYPRWSRKRIEETCWELLMNGYLNCEDLIDPVVTFANSPESYMQ
                     YVDQHPEQSIKMGVTF"
     gene            4935..5723
                     /locus_tag="PN977_001793"
     CDS             4935..5723
                     /locus_tag="PN977_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415830.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HDI6041829.1"
                     /translation="MKIGTQNQAFFPENILEKFRYIKEMGFDGFEIDGKLLVNNIEEV
                     KAAIKETGLPVTTACGGYDGWIGDFIEERRLNGLKQIERILEALAEVGGKGIVVPAAW
                     GMFTFRLPPMTSPRSLDGDRKMVSDSLRVLEQAAARTGTVVYLEPLNRYQDHMINTLA
                     DARRYIVENDLKHVQIIGDFYHMNIEEDNLAQALHDNRDLLGHVHIADNHRYQPGSGT
                     LDFHALFEQLRADNYQGYVVYEGRIRAEDPAQAYRDSLAWLRTC"
     gene            5733..6788
                     /locus_tag="PN977_001794"
     CDS             5733..6788
                     /locus_tag="PN977_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HDI6041830.1"
                     /translation="MKSAMTSSPLRVAIIGAGQVADKVHASYYCTRNDLELVAVCDSR
                     LSQAQALAEKYGNASVWDDPQAMLLAVKPDVVSVCSPNRFHYEHTLMELEAGCHVMCE
                     KPPAMTPEQAREMCDTARKQGKVLAYDFHHRFALDTQQLREQVTNGVLGEIYVTTARA
                     LRRCGVPGWGVFTNKELQGGGPLIDIGIHMLDAAMYVLGFPAVKSVTAHSFQKIGTQK
                     SCGQFGEWDPATYSVEDSLFGTIEFHNGGILWLETSFALNIREQSIMNVSFCGDKAGA
                     TLFPAHIYTDNNGELMTLMQREMADDNRHLRSMEAFINHVQGKPVMIADAEQGYIIQQ
                     LVAALYQSAETGTRVEL"
     gene            6785..9052
                     /locus_tag="PN977_001795"
     CDS             6785..9052
                     /locus_tag="PN977_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HDI6041831.1"
                     /translation="MTRPVTLTEPHFSQHTLNKYASLMAQGNGYLGLRASHEEDYTRQ
                     TRGMYLAGLYHRAGKNDINELVNLPDVIGMEITLNGELFALSRETWQRELDFASGELR
                     RNVVWSSASGARYAIASRRFVSAEQLPLMALEISITPLDADASVLISTGIDATQTNHG
                     RQHLDETQVRVFGQHLMQGIYTTQDGRSDVAISCCCQVSGDVQQCYTAKERRLLQHTS
                     AQLPAGKTLTLQKRVWIDWRDDRHVALDEWGSASLRQLEMCVQQSYDQLLAASTENWR
                     QWWQKRRITVNGGDAHDQQALDYALYHLRIMTPAHDERSSIAAKGLTGEGYKGHVFWD
                     TEVFLLPFHLFSEPTIARSLLRYRWHNLPGAQEKARRNGWQGALFPWESARSGEEETP
                     EFAAINIRTGLRQKVASAQAEHHLVADIAWAVVQYWQTTGDESFIAHEGMALLLETAK
                     FWISRTVRVNDRLEIHDVIGPDEYTEHVNNNAFTSYMAYYNVQQALNIARQFGCSDDA
                     FIHRAEMFLKELRLPEIQPDGVLPQDDSFMAKPAINLAKYKAAAGKQTILLDYSRAEV
                     NEMQILKQADVVMLNYMLPEQYSAASCLANLQFYEPRTIHDSSLSKAIHGIVAARCGL
                     LAQSYQFWREGTEIDLGADPHSCDDGIHAAATGAIWLGAIQGFAGASVRNGELHLNPA
                     LPEQWQQLSFPLFWQGCELQVTLDAQRIAIRTSAPVSLRLNGQLISVSEESVFCLGDF
                     ILPFNGTATTHQEGE"
     gene            9049..9708
                     /gene="pgmB"
                     /locus_tag="PN977_001796"
     CDS             9049..9708
                     /gene="pgmB"
                     /locus_tag="PN977_001796"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HDI6041832.1"
                     /translation="MKLQGVIFDLDGVITDTAHLHFQAWQQIAAEIGISIDAQFNEFL
                     KGISRDESLRRILQHGGKEGDFNPQERAQLAYRKNLLYVHSLRELTVNAVLPGIHNLL
                     VELRAQQIPVGLASVSLNAPTILAALELREFFTFCADASQLKNSKPDPEIFLAACAGL
                     GVPPQACIGIEDAQAGIEAINASGMRSVGIGAGLTGAQLLLPSTESLTWPRLSAFWQN
                     V"
     gene            9722..10804
                     /locus_tag="PN977_001797"
     CDS             9722..10804
                     /locus_tag="PN977_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDI6041833.1"
                     /translation="MAQLSLQHIQKIYDNQVHVVKDFNLEIADKEFIVFVGPSGCGKS
                     TTLRMIAGLEEISGGDLLIDGKRMNDVPAKARNIAMVFQNYALYPHMTVYDNMAFGLK
                     MQKIAKEVIDERVNWAAQILGLREYLKRKPGALSGGQRQRVALGRAIVREAGVFLMDE
                     PLSNLDAKLRVQMRAEISKLHQKLNTTMIYVTHDQTEAMTMATRIVIMKDGIVQQVGA
                     PKTVYNQPANMFVAGFIGSPAMNFIRGTIDGDKFVTETLKLTIPEEKLAVLKTQESLH
                     KPIVMGIRPEDIHPDAQEENNISAKISVAELTGAEFMLYTTVGGHELVVRAGALNDYH
                     AGENITIHFDMTKCHFFDAETEIAIR"
     gene            10849..11754
                     /gene="ompG"
                     /locus_tag="PN977_001798"
     CDS             10849..11754
                     /gene="ompG"
                     /locus_tag="PN977_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monomeric porin OmpG"
                     /protein_id="HDI6041834.1"
                     /translation="MKKLLPCTALVMCAGMACAQAEEKNDWHFNIGAMYEIENVEGYG
                     EDMDGLAEPSVYFNAANGPWRISLAYYQEGPVDYSAGKRGTWFDRPELEVHYQFLESD
                     DFSFGLTGGFRNYGYHYVDEPGKDTANMQRWKIAPDWDVKLTDDLRFNGWLSMYKFAN
                     DLNTTGYADTRVETETGLQYTFNETVALRVNYYLERGFNMDDSRNNGEFSTQEIRAYL
                     PLTLGNHSVTPYTRIGLDRWSNWDWLDDIEREGHDFNRVGLFYGYDFQNGLSVSLEYA
                     FEWQDHDEGDSDKFHYAGVGVNYSF"
     gene            complement(11805..12803)
                     /locus_tag="PN977_001799"
     CDS             complement(11805..12803)
                     /locus_tag="PN977_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDI6041835.1"
                     /translation="MSPTIYDIARVAGVSKSTVSRVLNKQTNISPEAREKVLRAIEEL
                     QYQPNKLARALTSSGFDAIMVISTRSTKTTAGNPFFSEVLHAITAKAEEEGFDVILQT
                     SHNLAEDLQKCESKIKQKMIKGIIMLSSPADESFFAQLDKYDIPVVVIGKVEGQYSHV
                     YSVDTDNYGDSIALTNALIESGHKNIACLHAPLDVHVSVDRVNGYKQSLATHNIAVRD
                     EWIVDGGYTHETALQAARQLLSQSPLPEAVFATDSLKLMSIYRAAAEKNIAIPQQLAV
                     VGYSNETLSFILTPAPGGIDVPTQELGQQSCELLFRLIAGKPSPQNITVATHMSLK"
     gene            12947..14344
                     /locus_tag="PN977_001800"
     CDS             12947..14344
                     /locus_tag="PN977_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcjX family protein"
                     /protein_id="HDI6041836.1"
                     /translation="MKRLKNELNALVNRGVDRHLRLAVTGLSRSGKTAFITAMVNQLL
                     NIHAGARLPLLSAVREERLLGVKRIPQRDFGIPRFTYDEGLAQLYGDPPAWPTPTRGV
                     SEIRLALRFKSNDSLLRHFKDTSTLYLEIVDYPGEWLLDLPMLAQDYLSWSRQMTGLL
                     NGQRGEWSAKWRMMCEGLDPLAPADENRLADIAAAWTEYLHHCKQQGLHFIQPGRFVL
                     PGDMAGAPALQFFPWPDVDAWGESKLAQADKHTNAGMLRERFNYYCEKVVKGFYKNHF
                     LRFDRQIVLVDCLQPLNSGPQAFNDMRLALTQLMQSFHYGQRTLFRRLFSPVIDKLLF
                     AATKADHVTIDQHANMVSLLQQLIQDAWQNAAFEGISMDCLGLASVQATTSGIIDVNG
                     EKIPALRGNRLSDGAPLTVYPGEVPARLPGQAFWDKQGFQFEAFRPQVMDVDKPLPHI
                     RLDAALEFLIGDKLR"
     gene            14341..15402
                     /locus_tag="PN977_001801"
     CDS             14341..15402
                     /locus_tag="PN977_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01620 family protein"
                     /protein_id="HDI6041837.1"
                     /translation="MTEPLKPRIDFDGPLDVDQNPEFRAQQTFDENQAQNFAPATLDE
                     APEEEGQVEAVMDAALRPKRSLWRKMVMGGLALFGASVVGQGVQWTMNAWQTQDWVAL
                     GGCAAGALIIGAGVGSVVTEWRRLWRLRQRAHERDEARDLLHSHGTGKGRAFCEKLAQ
                     QAGIDQSHPALQRWYASIHETQNDREVVSLYAHLVQPVLDAQARREISRSAAESTLMI
                     AVSPLALVDMAFIAWRNLRLINRIATLYGIELGYYSRLRLFKLVLLNIAFAGASELVR
                     EVGMDWMSQDLAARLSTRAAQGIGAGLLTARLGIKAMELCRPLPWLDDDKPRLGDFRR
                     QLIGQVKETLQKGKTPSEK"
     gene            15550..17091
                     /gene="tyrR"
                     /locus_tag="PN977_001802"
     CDS             15550..17091
                     /gene="tyrR"
                     /locus_tag="PN977_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TyrR"
                     /protein_id="HDI6041838.1"
                     /translation="MRLEVFCEDRLGLTRELLDLLVLRGIDLRGIEIDPIGRIYLNFA
                     ELEFESFSSLMAEIRRIAGVTDVRTVPWMPSEREHLALSALLEALPEPVLSVDMKSKV
                     DMANPASCQLFGQKLDRLRNHTAAQLINGFNFLRWLESEPQDSHNEHVVINGQNFLME
                     ITPVYLQDENDQHVLTGAVVMLRSTIRMGRQLQNVAAQDVSAFSQIVAVSPKMKHVVE
                     QAQKLAMLSAPLLITGDTGTGKDLFAYACHQASPRASKPYLALNCASIPEDAVESELF
                     GHAPEGKKGFFEQANGGSVLLDEIGEMSPRMQAKLLRFLNDGTFRRVGEDHEVHVDVR
                     VICATQKNLVELVQKGVFREDLYYRLNVLTLNLPPLRDCPQDIMPLTELFVARFADEQ
                     GVPRPKLAADLNTVLTRYAWPGNVRQLKNAIYRALTQLDGYELRPQDILLPDYDAATV
                     AVGEDAMEGSLDEITSRFERSVLTQLYRNYPSTRKLAKRLGVSHTAIANKLREYGLSQ
                     KKNEE"
     gene            complement(17135..17641)
                     /gene="tpx"
                     /locus_tag="PN977_001803"
     CDS             complement(17135..17641)
                     /gene="tpx"
                     /locus_tag="PN977_001803"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HDI6041839.1"
                     /translation="MSQTVHFQGNPVTVANSIPQAGSKAQTFTLVAKDLSDVTLGQFA
                     GKRKVLNIFPSIDTGVCAASVRKFNQLATEIDNTVVLCISADLPFAQSRFCGAEGLNN
                     VITLSTFRNAEFLQAYGVAIADGPLKGLAARAVVVIDENDNVIFSQLVDEITTEPDYE
                     AALAVLKA"
     gene            17760..18725
                     /gene="ycjG"
                     /locus_tag="PN977_001804"
     CDS             17760..18725
                     /gene="ycjG"
                     /locus_tag="PN977_001804"
                     /EC_number="5.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-Ala-D/L-Glu epimerase"
                     /protein_id="HDI6041840.1"
                     /translation="MRTVKVFEEAWPLHTPFVIARGSRSEARVVVVELEEEGIKGTGE
                     CTPYPRYGESDASVMAQIMSVVPQLEKGLTREELQKILPAGAARNALDCALWDLAARK
                     QQQSLAALIGITLPETVTTAQTIVIGTPDQMANSASTLWQAGAKLLKVKLDNHLISER
                     MVAIRTAVPDATLIVDANESWRAEGLAARCQLLADLGVAMLEQPLSAQDDAALENFIH
                     PLPICADESCHTRSNLKALKGRYEMVNIKLDKTGGLTEALALATEARAQGFRLMLGCM
                     LCTSRAISAALPLVPQVSFADLDGPTWLAVDVEPALQFTTGELHL"
     gene            complement(18700..19428)
                     /gene="mpaA"
                     /locus_tag="PN977_001805"
     CDS             complement(18700..19428)
                     /gene="mpaA"
                     /locus_tag="PN977_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415842.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide amidase MpaA"
                     /protein_id="HDI6041841.1"
                     /translation="MTVTRPRAERGAFPPGTEHYGRSLLGAPLIWFPAPAASHESGLI
                     LAGTHGDENSSIVTLSCALRTLTPSLRRHHVVLCVNPDGCQLGLRANANGVDLNRNFP
                     AANWKEGETVYRWNSAAEERDVVLLTGGKPGSEPETQALCQLIHRIQPAWVVSFHDPL
                     ACIEDPRHSELGEWLAQAFELPLVTSVGYETPGSFGSWCADLNLHCITAEFPPISSDE
                     ASEKYLFAMANLLRWHPKDAIRPS"
     gene            complement(19719..20357)
                     /locus_tag="PN977_001806"
     CDS             complement(19719..20357)
                     /locus_tag="PN977_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDI6041842.1"
                     /translation="MKNILILGAGGQIARHVINQLADKQTIKQTLFARQPAKIHKPYP
                     TNSKIIMGDVLNHAALKQAMQGQDVVYANLTGEDLDIQANSVIAAMKACDVKRLIFVL
                     SLGIYDEVPGKFGEWNNAVIGEPLKPFRRAADAIEASGLEYTILRPAWLTDEDIIDYE
                     LTSRNEPFKGTIVSRKSVAALITDIIDKPEKHIGENIGINQPGTDGDKPFFM"
     gene            complement(20428..21348)
                     /locus_tag="PN977_001807"
     CDS             complement(20428..21348)
                     /locus_tag="PN977_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309936.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDI6041843.1"
                     /translation="MMNNKVSFTNSNNPTISLSAVIYFPPKFDETRQYPVIVVSHPGG
                     GVKEQTAGTYAEKLAEKGFVTIAYDASYQGESGGEPRQLENPYIRTEDVSAVIDYLTT
                     LSYVDNTRIGAMGICAGAGYTANAAIQDRRIKAIGTVSAVNIGSMFRNGWENNVKSID
                     ALPYVDAGSDARTSDISSGEYATMPLAPMKESDAPNEELRQAWEYYHTPRAQYPTAPG
                     YATLRSLNQIITYDAYHMAEVYLTQPIQIVAGSQAGSKWMSDDLYDRASSKDKRYHIV
                     EGANHMDLYDGKVYVAEAISVLAPFFEETL"
     gene            21486..22385
                     /gene="pgrR"
                     /locus_tag="PN977_001808"
     CDS             21486..22385
                     /gene="pgrR"
                     /locus_tag="PN977_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator PgrR"
                     /protein_id="HDI6041844.1"
                     /translation="MKREEIADLMAFVVVAEERSFTRAAARLSMAQSALSQIVRRIEE
                     RLGLRLLTRTTRSVVPTEAGEHLLSVLGPMLHDIDSALTSLSDLQNRPSGTIRITTVE
                     HAAKTILLPAMRTFLKSHPEIDIQLTIDYGLTDVVSERFDAGVRLGGEMDKDMIAIRI
                     GPDIPMAIVGSPDYFSRRSAPTSVSQLIDHQAINLYLPTSGTANRWRLIRGGREVRVR
                     MEGQLLLNTIDLIIDAAIDGHGLAYLPYDQVERAIKEKKLIRVLDKFTPDLPGYHLYY
                     PHRRHAGSAFSLFIDRLKYKGAV"
     gene            22722..24335
                     /gene="mppA"
                     /locus_tag="PN977_001809"
     CDS             22722..24335
                     /gene="mppA"
                     /locus_tag="PN977_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide ABC transporter
                     substrate-binding protein MppA"
                     /protein_id="HDI6041845.1"
                     /translation="MKHSVSVTCCVLLVSSISLSYAAEVPSGTVLAEKQELVRHIKDE
                     PASLDPAKAVGLPEIQVIRDLFEGLVNQNEKGEIVPGVATQWKSNDSRIWTFTLRDNA
                     KWADGTPVTAQDFVYSWQRLVDPKTLSPFAWFAALAGINNAQAIIDGKATPDQLGVTA
                     VDAHTLKIQLDKPLPWFVNLTANFAFFPVQKANVESSKEWTKPGNLIGNGAYVLKERV
                     VNEKLVVVPNTHYWDNAKTVLQKVTFLPINQESAATKRYLAGDIDITESFPKNMYQKL
                     LKDIPGQVYTPPQLGTYYYAFNTQKGPTADQRVRLALSMTIDRRLMTEKVLGTGEKPA
                     WHFTPDVTAGFTPEPSPFEQMSQEELNAQAKTLLSAAGYGPQKPLKLTLLYNTSENHQ
                     KIAIAVASMWKKNLGVDVKLQNQEWKTYIDSRNTGNFDVIRASWVGDYNDPSTFLTLL
                     TSTHSGNISRFNNPAYDKVLAQASTENTVKARNADYNAAEKILMEQAPIAPIYQYTNG
                     RLIKPWLKGYPINNPEDVAYSRTMYIVKH"
     gene            complement(24386..25417)
                     /gene="ynaI"
                     /locus_tag="PN977_001810"
     CDS             complement(24386..25417)
                     /gene="ynaI"
                     /locus_tag="PN977_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low conductance mechanosensitive channel YnaI"
                     /protein_id="HDI6041846.1"
                     /translation="MIAELFTNNALNLVIIFGSCAALILMSFWFRRGNRKRKGFLFHA
                     VQFLIYTIIISAVGSIINYVIENYKLKFITPGVIDFICTSLIAVILTIKLFLLINQFE
                     KQQIKKGRDITSARIMSRIIKITIIVVLVLLYGEHFGMSLSGLLTFGGIGGLAVGMAG
                     KDILSNFFSGIMLYFDRPFSIGDWIRSPDRNIEGTVAEIGWRITKIKTFDNRPLYVPN
                     SLFSSISVENPGRMTNRRITTTIGLRYEDAAKVGVIVEAVREMLKNHPAIDQRQTLLV
                     YFNQFADSSLNIMVYCFTKTTVWAEWLAAQQDVYLKIIDIVQSHGADFAFPSQTLYMD
                     NITPPDQGR"
     gene            25661..25918
                     /locus_tag="PN977_001811"
     CDS             25661..25918
                     /locus_tag="PN977_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2534 family protein"
                     /protein_id="HDI6041847.1"
                     /translation="MIMAKLKSAKGKKFLFGLLAVFIIAASVVTRATIGGVIEQYNIP
                     LSEWTTSMYVIQSSMIFVYSLVFTVLLAIPLGIYFLGGEEQ"
     gene            complement(25968..26918)
                     /gene="uspE"
                     /locus_tag="PN977_001812"
     CDS             complement(25968..26918)
                     /gene="uspE"
                     /locus_tag="PN977_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspE"
                     /protein_id="HDI6041848.1"
                     /translation="MAMYQNMLVVIDPNQDDQPALRRAVYLHQRIGGKIKAFLPIYDF
                     SYEMTTLLSPDERTAMRQGVISQRTAWIHEQAKYYLNAGVPIEIKVVWHNRPFEAIIQ
                     EVISGGHDLVLKMAHQHDRLEAVIFTPTDWHLLRKCPSPVWMVKDQPWPEGGKALVAV
                     NLASEEPYHNALNEKLVKETIELAEQVNHTEVHLVGAYPVTPINIAIELPEFDPSVYN
                     DAIRGQHLLAMKALRQKFGINENMTHVEKGLPEEVIPDLAEHLQAGIVVLGTVGRTGI
                     SAAFLGNTAEQVIDHLRCDLLVIKPDQYQTPVELDDEEDD"
     gene            complement(27070..27822)
                     /gene="fnr"
                     /locus_tag="PN977_001813"
     CDS             complement(27070..27822)
                     /gene="fnr"
                     /locus_tag="PN977_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007849744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate/nitrate reduction transcriptional
                     regulator Fnr"
                     /protein_id="HDI6041849.1"
                     /translation="MIPEKRIIRRIQSGGCAIHCQDCSISQLCIPFTLNEHELDQLDN
                     IIERKKPIQKGQTLFKAGDELKSLYAIRSGTIKSYTITEQGDEQITGFHLAGDLVGFD
                     AIGSGHHPSFAQALETSMVCEIPFETLDDLSGKMPNLRQQMMRLMSGEIKGDQDMILL
                     LSKKNAEERLAAFIYNLSRRFAQRGFSPREFRLTMTRGDIGNYLGLTVETISRLLGRF
                     QKSGMLAVKGKYITIENNDALAQLAGHTRNVA"
     gene            complement(28017..28532)
                     /gene="ogt"
                     /locus_tag="PN977_001814"
     CDS             complement(28017..28532)
                     /gene="ogt"
                     /locus_tag="PN977_001814"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HDI6041850.1"
                     /translation="MLRLLEEKIVTPLGPLWVICDEQFRLRAVEWEEYSERMVQLLDI
                     HYRKEGYERISATNPGGLSDKLREYFAGNLSIIDTLPTATGGTPFQREVWKTLRTIPC
                     GQVMHYGQLAEQLGRPGAARAVGAANGSNPISIVVPCHRVIGRNGTMTGYAGGVQRKE
                     WLLRHEGYLLL"
     gene            complement(28543..30069)
                     /gene="abgT"
                     /locus_tag="PN977_001815"
     CDS             complement(28543..30069)
                     /gene="abgT"
                     /locus_tag="PN977_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-aminobenzoyl-glutamate transporter"
                     /protein_id="HDI6041851.1"
                     /translation="MSMSSIPSSSQSGKLYGWVERIGNKVPHPFLLFIYLIIVLMVTT
                     AILSAFGVSAKNPADGTPVVVKNLLSVEGLHWFLPNVIKNFSGFAPLGAILALVLGAG
                     LAERVGLLPALMVKMASHVNARYASYMVLFIAFFSHISSDAALVIMPPMGALIFLAVG
                     RHPVAGLLAAIAGVGCGFTANLLIVTTDVLLSGISTEAAAAFNPQMHVSVIDNWYFMA
                     SSVVVLTIVGGLITDKIIEPRLGQWQGNSDEKLQTLTESQRFGLRIAGVVSLLFIAAI
                     ALMVIPENGILRDPINHTVMPSPFIKGIVPLIILFFFVVSLAYGIATRTIRRQADLPH
                     LMIEPMKEMAGFIVMVFPLAQFVAMFNWSNMGKFIAVGLTDILESSGLSGIPAFVGLA
                     LLSSFLCMFIASGSAIWSILAPIFVPMFMLLGFHPAFAQILFRIADSSVLPLAPVSPF
                     VPLFLGFLQRYKPDAKLGTYYSLVLPYPLIFLVVWLLMLLAWYLVGLPIGPGIYPRLS
                     "
     gene            complement(30106..31551)
                     /gene="abgB"
                     /locus_tag="PN977_001816"
     CDS             complement(30106..31551)
                     /gene="abgB"
                     /locus_tag="PN977_001816"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-aminobenzoyl-glutamate hydrolase subunit AbgB"
                     /protein_id="HDI6041852.1"
                     /translation="MQEIYRFIDDAIEADRQRYTDIADQIWDHPETRFEEFWSAEHLA
                     SALESAGFTVTRNVGNIPNAFIASFGQGKPVIALLGEYDALAGLSQQASCAQPTSATP
                     GENGHGCGHNLLGTAAFAAAIAVKKWLEQYGQGGTVRFYGCPGEEGGSGKTFMVREGV
                     FDDVDAALTWHPEAFAGMFNTRTLANIQASWRFKGIAAHAANSPHLGRSALDAVTLMT
                     TGTNFLNEHIIEKARVHYAITDSGGISPNVVQAQAEVLYLIRAPEMTDVQHIYDRVAK
                     IAEGAALMTETTVECRFDKACSSYLPNRTLENAMYRALSHFGTPEWNCEELAFAKQIQ
                     ATLTPNDRQNSLNNIAATGGENGKAFALRHRETVLANEVAPYAATDNVLAASTDVGDV
                     SWKLPVAQCFSPCFAVGTPLHTWQLVSQGRTSIAHKGMLLAAKTMAATTLNLFIDSGL
                     LQECQQEHQQVTDTQPYHCPIPKNVTPSPLK"
     gene            complement(31551..32861)
                     /gene="abgA"
                     /locus_tag="PN977_001817"
     CDS             complement(31551..32861)
                     /gene="abgA"
                     /locus_tag="PN977_001817"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-aminobenzoyl-glutamate hydrolase subunit AbgA"
                     /protein_id="HDI6041853.1"
                     /translation="MESLNQFINSLAPKLSHWRRDFHHYAESGWVEFRTATVVAEELH
                     QLGYSLALGREVVNESSRMGLPDEFTLQREFERARQQGALEQWIAAFEGGFTGIVATL
                     DTGRPGPVMAFRVDMDALDLSEEQDVSHRPYRDGFASCNAGMMHACGHDGHTAIGLGL
                     AHTLKQFESGLHGVIKLIFQPAEEGTRGARAMVDAGVVDDVDYFTAVHIGTGVPAGTV
                     VCGSDNFMATTKFDAHFTGTAAHAGAKPEDGHNALLAAAQATLALHAIAPHSEGASRV
                     NVGVMQAGSGRNVVPASALLKVETRGASDVINQYVFERAQQAIQGAATMYGVGVETRL
                     MGAATASSPSPQWVAWLQIQAAQVAGVNQAIERVEAPAGSEDATLMMARVQRHQGQAS
                     YMVFGTQLAAGHHNEKFDFDEQVLAIAVETLARTALNFPWTRGI"
     gene            33037..33945
                     /locus_tag="PN977_001818"
     CDS             33037..33945
                     /locus_tag="PN977_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDI6041854.1"
                     /translation="MAFQVKIHQIRAFVEVARQGSIRGASRMLNMSQPALSKSIQELE
                     EGLAAQLFFRRSKGVTLTDAGESFYQHASLILEELRAAQEDIRQRQGQLAGQINIGMG
                     ASISRSLMPAVISRFHQQHPQVKVRIMEGQLVSMINELRQGELDFTINTYYQGPYDHE
                     FTFEKLLEKQFAIFCRPGHPAIGARSIKQLLDYSWTMPTPHGSYYKQLSELLDDQAQT
                     PQVGVVCETFSACISLVAKSDFLSILPEEMGCDPLHGQGLVMLPVSEILPKATYYLIQ
                     RRDSRQTPLTASLITQFRRECGYLQS"
     gene            34276..34839
                     /gene="smrA"
                     /locus_tag="PN977_001819"
     CDS             34276..34839
                     /gene="smrA"
                     /locus_tag="PN977_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA endonuclease SmrA"
                     /protein_id="HDI6041855.1"
                     /translation="MNLDDKSLFLDAMEDVQPLKRATDVHWHPTRNQRAPQRIDTLQL
                     DNFLTTGFLDIIPLTQPLEFRREGLQHGVLDKLRSGKYPQQASLNLLRQPVEECRKMV
                     FSFIQQALADGLRNVLIIHGKGREDKSHANIVRSYVARWLTEFDDVQAYCTALPHHGG
                     SGACYVALRKTAQAKQENWERHAKRSR"
     gene            complement(34860..36092)
                     /gene="dgcM"
                     /locus_tag="PN977_001820"
     CDS             complement(34860..36092)
                     /gene="dgcM"
                     /locus_tag="PN977_001820"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcM"
                     /protein_id="HDI6041856.1"
                     /translation="MITHNFNTLDLLTSPVWIVSPFEEQLIYANSAARLLMQDLTFSQ
                     LRTGPYSVSSQKELPKYLSDLQNQHDIIEILTVQRKEEETALSCRLVLRELTEAEPVI
                     IFEGIEAPATLGLKASRSANYQRKKQGFYARFFLTNSAPMLLIDPSRDGQIVDANLAA
                     LNFYGYNHETMCQKHTWEINMLGRRVMPIMHEISHLPGGHKPLNFVHKLADGSTRHVQ
                     TYAGPIEIYGDKLMLCIVHDITEQKRLEEQLEHAAHHDAMTGLLNRRQFYHITEPGQM
                     QHLAIAQDYSLLLIDTDRFKHINDLYGHSKGDEVLCALARTLESCARKGDLVFRWGGE
                     EFVLLLPRTPLDTALSLAETIRVSVAKVSISGLPRFTVSIGVAHHEGNESIDELFKRV
                     DDALYRAKNDGRNRVLAA"
BASE COUNT         9095 a         8797 c         9469 g         9025 t
ORIGIN      
        1 agttctcgct gccgccgctg gtgctgcatg cggtgcaaaa acagaacgtt gaggcgcttt
       61 gtgcgtgggc gcaaaacctg acactacctt ccagcaacac cacctggttt aacttcctcg
      121 cctctcacga tggcatcggg cttaacccac tgcgtggctt gctacccgaa agcgaaatat
      181 tagcgctggt cgaggcatta cagcaggaag gggcattagt taactggaaa aataatcccg
      241 acggtacgcg tagcccgtat gaaatgaatg tcacttatat ggatgcgtta aaccgccgcg
      301 agagtagcga tgaagaacgt tgcgccaggt ttatccttgc ccatgcgatt ttgttaagtt
      361 tccccggtgt gccagcgata tatattcaaa gtattctggg ctcgcgtaat gattacgcag
      421 gtgtcgaaaa actgggatat aaccgtgcga ttaaccgtaa aaaatattac agtaaagaga
      481 tcacgaccga actgaacaat aaaacgacgt taaggcacgc ggtatatcat gaattgtcgc
      541 gactaattaa aattcgtcga agtcataacg agtttcatcc agataatgat tttaccatcg
      601 acacggttaa ttcatccgta atgtgtattc aaagaagcaa cgcggatggt aattgtctga
      661 caggattgtt taatgtcagt gaaaatattc agcatataaa tattactgac ctgcacggtc
      721 gggatctgat tagtgaagtt gatatagtgg gtaatgaaat aacgctgcgc ccctggcagg
      781 ttatgtggat taaataaaaa ggaacatctc atgattaaat caaaaatcgt gctgttatca
      841 gcactggttt cctgcgccct gatttcaggt tgtaaagaag aaaataaaac gaatgtctcc
      901 attgagttta tgcattcttc ggtggaacag gaacgccagg cggtaattag tcagttaatt
      961 gcgcgttttg aaaaagaaaa cccgggcatc accgttaaac aagtgcccgt ggaagaagac
     1021 gcctataaca ccaaagtcat taccctttca cgtagtggtt cgctgccgga agtgatcgaa
     1081 accagccatg actacgccaa agttatggac aaagagcagc ttatcgatcg cctggcggtt
     1141 gccacggtca tcagcaacgt cggtgaaggc gcgttctacg atggcgttct gcggattgtg
     1201 cgcaccgaag atggtagcgc atggaccggt gttcctgtca gcgcctggat tggcggcatc
     1261 tggtatcgca aagatgtgct ggcaaaagcc gggctggagg agccgaaaaa ctggcagcaa
     1321 ttgctggatg tcgcgcagaa actgaatgac ccggcgaata aaaaatatgg cattgcgctg
     1381 cctacggcag aaagtgtgct gacggaacaa tccttctccc agtttgcctt atccaaccag
     1441 gccaacgtct ttaacgccga aggcaaaatc acccttgata caccagagat gatgcaggcg
     1501 ctgacctatt accgcgacct tgctgccaac accatgccgg gttctaacga catcatggag
     1561 gtgaaagacg cttttatgaa cggcaccgcg ccgatggcga tttactccac ctatatcctt
     1621 ccggctgtga ttaaagaggg cgatccgaaa aacgttggtt tcgttgtacc caccgagaaa
     1681 aactctgcgg tctacggcat gttgacctcg ctgaccatta ccgccgggca aaagaccgaa
     1741 gagaccgaag cggctgaaaa atttgtcacc tttatggagc aggcagacaa cattgccgac
     1801 tgggtgatga tgtcgccagg cgcggcgctg ccggtgaaca aagcggttgt cactaccgcc
     1861 acctggaaag acaacgacgt tattaaggcg ctgggagaat tgccaaatca gttaatcagc
     1921 gaactgccaa atattcaggt ctttggcgca gtaggggata aaaattttac ccgcatgggc
     1981 gatgtgacgg gttctggcgt ggtgagctcc atggtgcata acgtcaccgt gggtaaagcc
     2041 gacctctcta ccacgctgca aacgagccag aaaaagctgg atgaactgat cgaacagcac
     2101 taatcccagg acaggaatcc gcaatgaaca ggcttttttc aggtcgttcc gatatgccct
     2161 ttgcgctgct gcttctcgcg cccagcttat tactgctggg cggtctggtg gcgtggccaa
     2221 tggtgtcgaa tatcgaaatc agttttttac gtctgccgct taatcccaac atcgagtcaa
     2281 cgtttgttgg ggtgagcaac tatgtgcgta ttctctccga tcccggcttc tggcactcgc
     2341 tgtggatgac ggtctggtat accgcgctgg tggtggcagg tagcaccgtg ctcgggctgg
     2401 cagtggcgat gtttttcaac cgcgagttcc gcctgcgtaa aaccgcgcgt tcgctggtga
     2461 tcctctccta cgtaacgccg tctatttcgc tggtgttcgc ctggaaatac atgttcaaca
     2521 acggctacgg cattgtgaac tacctcggcg tcgatcttct gcatctctat gagcaggcac
     2581 cgctgtggtt cgataatccg ggcagtagct ttgtgctggt ggtgctgttc gccatctggc
     2641 gctacttccc gtatgccttt atctcgtttc tggcgatttt gcagaccatt gataaatcgt
     2701 tgtacgaagc ggcagagatg gacggcgcga atgcctggca acggtttcgc atcgtcacgc
     2761 tgcccgcgat tatgccggtc ctggcgacgg tggtgacgct gcgcaccatc tggatgttct
     2821 acatgtttgc tgatgtttat ctgctgacga ccaaggtcga tattctcggc gtctatctct
     2881 acaaaaccgc ctttgccttt aatgacttag ggaaagcggc ggcgatctcg gtggtgctct
     2941 tcatcattat tttcgctgtc attctgctga ccagaaaacg ggtgaacctc aatggcaaca
     3001 aataaacgca cactcagtcg catcggtttt tactgcgggc tggcgctgtt tctcatcatc
     3061 acgctgtttc cgttttttgt gatgctgatg acctcgttca aaagcgcgaa agaggcgatc
     3121 tcgctgcatc ctacgctgct gccacagcag tggacgctgg agcattacgt cgacattttt
     3181 aacccggtga ttttcccgtt tgtcgattat ttccgtaaca gcatggtggt gtcggtagtt
     3241 tcatccgtga tagcagtatt tctcggcatt ctcggggctt acgcgctttc ccgcctgcgc
     3301 tttaaagggc ggatgaccat caacgccagc ttttacacgg tgtacatgtt ctccggcatt
     3361 ttgctggtgg tgccgctgtt caaaatcatc accgcgcttg gcatatacga caccgagatg
     3421 gcgctgatca tcaccatggt aacgcaaacc ctgcccaccg ccgtgtttat gctgaaaagc
     3481 tacttcgaca ccatccctga tgagattgaa gaagcggcga tgatggatgg cctcaaccgg
     3541 ctgcaaatca tcttccgcat taccgtgccg ctggcaatgt ccgggctgat ctccgtgttc
     3601 gtctactgct ttatggtggc gtggaacgac tatctgtttg cgtcgatttt cctctccagc
     3661 gccagcaatt tcaccttacc agtgggcctg aacgcgctgt tcagtacgcc tgattacatc
     3721 tggggacgca tgatggcggc ctcgctggtg accgcattgc cggtggtgat tatgtatgcg
     3781 ctttcggaac gttttattaa gagtggtttg accgccggtg gcgtgaaggg ctaaagcggc
     3841 cagtttcttt acaaggagtt ttacatgaaa aagttagtag ccacagcacc gcgcgttgct
     3901 gcgctggttg agtatgaaga tcgggcgatt ttagccaatg aagtgaagat ccgcgtgcgt
     3961 ttcggcgcac cgaaacacgg aacggaagtg gtcgacttcc gcgccgccag cccgtttatc
     4021 gatgaagact ttaacggcga gtggcagatg ttcaccccgc gtcccgcaga tgcgccgcgc
     4081 ggcattgagt ttggcaaatt ccagcttggc aacatggtgg ttggcgacat tatcgagtgc
     4141 ggcagcgacg ttaccgacta cgcggtgggc gacagcgtat gcggctacgg cccgctctcc
     4201 gagacggtca tcattaacgc agtgaataac tacaagctgc gcaaaatgcc gcaaggcagc
     4261 tcctggaaaa acgccgtctg ttacgacccg gcgcagtttg ccatgagcgg cgtgcgcgat
     4321 gccaacgtac gcgtagggga ttttgtcgtg gtggtagggc ttggcgcgat cggtcaaatt
     4381 gccatccaac tggcgaaacg cgctggcgca tcggtggtaa ttggcgtcga tcctatcgcc
     4441 catcgctgtg agattgcccg tcgccacggt gctgatttct gccttaaccc cattggcact
     4501 gacgtaggca aagagatcaa aacgctgacc ggcaagcagg gtgccgatgt gattatcgaa
     4561 accagcggtt acgccgacgc gctgcaatcg gcgctgcgcg gtctgtccta cggcggcacc
     4621 atctcgtatg tcgcgtttgc caaaccattt gctgaaggtt ttaacctcgg acgcgaagcg
     4681 catttcaata acgccaaaat tgttttctcc cgcgcgtgca gcgaaccgaa cccggattat
     4741 ccgcgctgga gccgtaagcg tattgaagaa acctgctggg aactgctgat gaacggttat
     4801 ctcaattgcg aagatttaat cgacccggta gtgacctttg ccaacagccc ggaaagctac
     4861 atgcagtatg tcgatcagca tccggaacag agcatcaaaa tgggcgttac gttttaattc
     4921 aaaggaatga aaaaatgaaa ataggcacac agaatcaggc attctttccg gaaaatattc
     4981 tggagaaatt tcgttatatc aaagagatgg gcttcgatgg ttttgagatt gacggcaaac
     5041 tgctggtcaa caacatcgaa gaagtcaaag cggcaatcaa agaaaccggt ttaccggtga
     5101 ccaccgcctg cggtggctat gacggttgga ttggcgactt tatcgaagag cgtcgactta
     5161 atggcttaaa gcagatcgaa cgcattcttg aagctctggc agaagtgggc ggtaaaggta
     5221 tcgtcgttcc ggctgcatgg ggcatgttta ccttccgctt accgccgatg acctcgccgc
     5281 gtagcctgga tggcgaccgc aaaatggtga gtgattccct gcgcgtactg gaacaggcgg
     5341 ccgcacgtac cggaaccgtg gtgtacctcg aaccgttaaa ccgctatcag gatcacatga
     5401 tcaacaccct cgccgatgct cgtcgttaca tcgtcgaaaa cgatcttaaa catgtacaga
     5461 ttatcggcga tttctatcac atgaatatcg aagaagataa cctggcgcag gcgctgcatg
     5521 acaaccgcga cctgctcggt catgtgcata ttgccgataa ccatcgctac cagccgggca
     5581 gcggcaccct ggatttccac gcgctgtttg aacagctgcg cgcggataac tatcagggct
     5641 atgtggtgta cgaagggcgt atccgggcag aagatcctgc ccaggcgtac cgtgattctt
     5701 tggcctggtt gcgtacctgc taagaggtct ttgtgaaaag tgcaatgaca agctctccgc
     5761 tgcgggtcgc gatcataggc gcgggccagg tggcggataa agttcatgct tcgtactact
     5821 gcacccgcaa cgatctggaa ctggtggctg tctgtgacag ccgcctttcc caggcgcagg
     5881 cgctggcaga aaaatacggg aatgcatccg tgtgggacga tccgcaggcc atgctgctgg
     5941 cggtgaaacc tgatgtggtt agcgtctgct cacctaaccg ttttcattac gaacataccc
     6001 tgatggagct ggaagcgggc tgccatgtga tgtgcgaaaa accgcccgcc atgacgccag
     6061 aacaggcgcg ggaaatgtgc gataccgcgc gcaaacaggg caaggtgctg gcctacgact
     6121 ttcaccatcg ttttgcactc gatacgcaac agctgcgtga acaggtgacc aacggcgttt
     6181 tgggagagat ttacgttacc accgcccgcg ccctgcgtcg ctgcggcgtt cccggctggg
     6241 gcgtttttac caataaagaa ttgcagggcg gcgggccgct tattgacatc ggcattcata
     6301 tgctggatgc ggcgatgtat gtactgggct ttccggcggt gaaaagcgtg accgcgcata
     6361 gctttcaaaa gatcggcaca caaaagagct gcggtcaatt tggcgagtgg gacccggcaa
     6421 cctacagcgt cgaagattcg ctgtttggca ccattgaatt tcataacggc ggcattctgt
     6481 ggcttgagac ctcatttgca ctcaacatcc gcgaacagtc gattatgaat gtcagctttt
     6541 gtggtgataa agccggggcg acgctgtttc cagcacatat ctacaccgat aacaacggtg
     6601 agttaatgac gctgatgcaa cgggaaatgg cagacgacaa ccgtcatctg cgcagtatgg
     6661 aagcctttat caaccacgta caaggtaagc ccgtgatgat agccgacgcc gagcaggggt
     6721 acatcatcca gcaactggtg gcggcgttgt atcaatccgc agaaacaggg acgcgtgtgg
     6781 aattatgacc aggccagtaa cgttaacgga accccatttc agccagcata ccctgaacaa
     6841 gtatgcttcg ctgatggcgc aggggaacgg ctatcttggg cttcgcgcca gccatgaaga
     6901 agattatacg cgccagacac gcggtatgta tctggcgggg ctttatcatc gcgcggggaa
     6961 aaatgacatc aacgaactgg tgaacctgcc tgacgtcata gggatggaga ttaccttgaa
     7021 tggtgaactt tttgcgctat cccgcgaaac gtggcagcgc gagcttgact tcgccagtgg
     7081 agaattacgt cgcaatgtcg tctggtcttc agccagcggc gcacgttatg ccatcgccag
     7141 ccgtcgcttt gtttcggcag aacaactgcc gctgatggcg ctggaaatca gcattacgcc
     7201 gctggacgct gacgcgtcag tgctgatttc aacaggcatt gacgccacgc aaaccaacca
     7261 cggacgacaa catctcgacg aaacccaggt gcgggtgttt ggtcagcatt tgatgcaggg
     7321 gatctatacc actcaggatg gacgcagtga tgtggccatc agctgttgct gccaggtgag
     7381 cggtgacgtg cagcaatgct ataccgccaa agagcgccgc ttgctgcaac ataccagtgc
     7441 acaactacct gcgggcaaaa cgctgacgct gcaaaaacgg gtgtggatcg actggcggga
     7501 cgacagacac gtcgctttag acgagtgggg tagtgcatcg cttcgtcagc ttgaaatgtg
     7561 tgtacagcag agttacgacc aacttcttgc agcatccaca gaaaactggc gtcaatggtg
     7621 gcagaaacgt cgtatcacgg taaacggtgg cgatgcgcac gatcagcaag cgttagatta
     7681 tgcgctttat cacctacgca tcatgacgcc tgctcacgac gagcgcagca gtattgcggc
     7741 aaaaggtttg accggggaag gctacaaagg ccacgttttc tgggatacgg aagtattttt
     7801 actgccgttc catctgttta gcgaaccgac gattgccaga agtttactgc gttatcgctg
     7861 gcacaacttg ccaggcgcgc aggagaaagc gcgacgcaac ggctggcagg gcgcgctatt
     7921 tccgtgggaa agcgcgcgca gcggcgaaga agagacgccg gaatttgccg ccattaacat
     7981 tcgcaccggg ctgcggcaaa aagtggcctc ggcgcaggcg gaacatcatc tggtcgccga
     8041 tatcgcctgg gcggttgttc aatactggca gaccacgggg gatgaaagtt tcattgctca
     8101 tgaaggcatg gcgctacttc tggaaactgc aaagttctgg attagccgca cggtgagggt
     8161 taacgaccgt ctggaaattc atgatgttat tgggccagac gaatataccg aacatgtcaa
     8221 taataacgcc ttcaccagct atatggcgta ttacaacgtc cagcaggcgc tgaacatcgc
     8281 tcgtcaattt ggctgtagcg acgatgcgtt tatccatcgc gccgaaatgt tccttaaaga
     8341 actgcggctg ccagaaattc agcccgacgg cgttttgccg caggatgatt cgtttatggc
     8401 gaaaccagcg attaatctgg cgaaatacaa agcggcggcg gggaagcaaa ccattctgct
     8461 ggattattca cgcgcagaag tgaacgagat gcagatcctc aaacaagctg atgtggtgat
     8521 gctcaattac atgctgccgg agcagtactc agcggcatcg tgtcttgcca atctgcaatt
     8581 ttatgaaccg cgcaccattc acgactcttc actgagtaaa gcgatccacg gcattgttgc
     8641 cgcacgctgt ggcctgttgg cgcaaagtta tcagttctgg cgggagggga ctgaaatcga
     8701 tcttggtgct gatccgcata gctgtgatga cggtatccac gctgccgcaa ctggcgcaat
     8761 ctggttgggg gcgattcagg gttttgccgg ggcgagcgtg cgcaacggtg aattacatct
     8821 caatccggcg ttacctgagc agtggcaaca gttgtctttc cctctgttct ggcagggctg
     8881 tgaattacag gtcactcttg acgcgcagcg cattgcgatt cggacttctg cgccagtttc
     8941 actgcgtttg aacggtcagc ttatttcagt ctctgaagaa tctgttttct gtttagggga
     9001 ttttattttg cccttcaatg ggaccgctac cacgcatcag gagggtgaat gaaactgcaa
     9061 ggggtaattt tcgatctgga tggagtaatc accgataccg cacatctgca tttccaggcg
     9121 tggcagcaga ttgccgctga aattggcatc agcattgatg cgcagtttaa cgaattccta
     9181 aaagggatca gccgcgatga gtccctgcgg cgaattctgc agcacggggg taaagagggc
     9241 gattttaacc cacaggaacg ggcgcaactg gcgtatcgca aaaatctgct ttacgtccac
     9301 tcactgcgcg aactgacggt taatgctgtg ctgcctggca ttcacaattt gctggtagag
     9361 ctccgagcac agcagattcc ggttgggctg gcttctgtct ccctgaatgc gccgacgatt
     9421 ttagcagcgc tggagctgcg cgagtttttc accttctgcg cggacgcttc ccaactcaaa
     9481 aattcgaaac cggacccgga aatctttctc gccgcctgtg cagggctggg cgtgccgcca
     9541 caggcatgta tcggcattga agatgcgcag gcgggcattg aagccattaa cgccagcggt
     9601 atgcgctcgg tggggatcgg cgcgggctta accggggcgc aattgctgtt gccctcaacg
     9661 gaatctctca catggccgcg gttatcggcc ttctggcaaa acgtatagca aaggaatcaa
     9721 catggctcag ctttcgttac aacatattca aaaaatctac gataaccagg tgcatgtggt
     9781 gaaggacttc aacctggaaa ttgccgataa agagttcatt gtctttgtcg gtccgtcagg
     9841 ctgcggtaaa tcgactacgc tgcgcatgat tgccgggctt gaggagatca gcggtggcga
     9901 tctgttgatc gacggcaaac gaatgaatga cgttccagcc aaagcgcgca atatcgcgat
     9961 ggtgttccag aactacgcgc tgtatccgca tatgacggtc tacgacaaca tggcgtttgg
    10021 cctgaagatg caaaaaatcg ccaaagaggt gattgatgag cgggttaact gggcggcgca
    10081 aatcctcggc ctgcgtgagt acctgaaacg caagccgggc gcgctttctg gcgggcaacg
    10141 ccagcgtgtg gcgctcggac gggcgattgt gcgcgaagcg ggcgtgtttt taatggatga
    10201 accgctctct aaccttgatg ccaagctgcg cgtacaaatg cgcgcagaga tcagcaagct
    10261 gcatcagaaa ctgaacacca ccatgatcta cgtgacccac gatcagaccg aagcgatgac
    10321 catggcgacg cggattgtga ttatgaagga tgggattgtt cagcaggtcg gtgcgccgaa
    10381 aacggtttat aaccaacctg cgaatatgtt tgttgccgga tttattggat cgcctgcgat
    10441 gaattttatt cgcggcacga tcgatggcga taaattcgtt acggaaacgc ttaaattaac
    10501 catacccgaa gagaaattag cggttctgaa aacacaggaa agtttgcata agcccatcgt
    10561 gatgggaata cgcccggaag atattcatcc ggacgcgcaa gaggaaaata acatttccgc
    10621 caaaattagc gtggcagaat taaccggtgc ggaatttatg ctctacacca cggttggggg
    10681 gcacgagtta gtggtccgtg ctggtgcgtt aaatgattat catgcaggag aaaatatcac
    10741 tattcatttt gatatgacta aatgtcattt ctttgatgca gaaacggaaa tagcaattcg
    10801 ctaaatacag ggggaaggca ttcccccagg ataatacaag gaacaataat gaaaaagtta
    10861 ttaccctgta ccgcactggt gatgtgtgcg ggaatggctt gcgcacaggc cgaggaaaag
    10921 aacgactggc actttaatat cggcgcgatg tacgaaatag aaaacgtcga aggttatggc
    10981 gaagatatgg atgggctcgc ggagccttct gtctatttta atgccgccaa cgggccgtgg
    11041 agaatttctc tggcctatta tcaggaaggg ccggtagatt atagcgcggg taaacgtgga
    11101 acgtggtttg atcgcccgga gctggaggta cattatcagt tcctcgaaag cgatgatttc
    11161 agtttcggtc tgactggtgg tttccgtaat tatggttatc actacgttga tgaaccgggt
    11221 aaagacacgg cgaatatgca gcgttggaaa atcgcgccag actgggatgt gaaactgact
    11281 gacgatttac gtttcaacgg ttggttatcg atgtataaat ttgccaacga tctgaacacg
    11341 accggttacg ctgatacccg agtcgaaacg gaaacaggtc tgcaatatac cttcaacgaa
    11401 acggttgctt tgcgagtgaa ctattatctc gagcgcggct tcaatatgga cgacagccgc
    11461 aataacggtg agttttccac gcaagaaatt cgtgcctatt tgccgctgac gctcggcaac
    11521 cactcggtga cgccgtatac gcgcattggg ctggatcgct ggagtaactg ggactggctg
    11581 gatgatattg aacgtgaagg ccatgatttt aaccgtgtgg ggttatttta cggttatgat
    11641 ttccagaatg gtctttccgt ttcgctggaa tacgcgtttg agtggcagga tcacgacgaa
    11701 ggcgacagcg ataaattcca ttatgcaggt gtcggcgtaa attactcgtt ctgataatgg
    11761 gttaaattgc cggatgcggc gcgagcgcca aatccggcat acatttattt caacgacata
    11821 tgcgtggcaa cggtaatatt ttgtggtgac ggttttccgg caattaagcg gaataataac
    11881 tcgcagcttt gttgccctaa ctcctgcgtc ggaacatcga tgccgcccgg tgcaggcgtt
    11941 aaaataaatg acagcgtttc attgctataa cccaccaccg ctaactgctg cggaatagca
    12001 atatttttct ctgctgccgc acgataaatg ctcattaatt tcaggctgtc agtggcaaat
    12061 acggcttctg gcaacggcga ctggcttaat aattgccgtg cggcttgcaa ggctgtttca
    12121 tgggtataac cgccgtcaac aatccattca tcacgcactg caatattatg cgtagccagg
    12181 ctttgcttat aaccatttac ccgatccact gaaacatgaa catcaagcgg cgcatgcagg
    12241 caggcaatat tcttatgccc gctttcaatt aacgcattgg tcagcgcaat actgtcgcca
    12301 taattatcgg tatcgacgga ataaacatgg ctgtattgac cttcaacttt gccaattacc
    12361 actaccggaa tatcatattt atcgagttgg gcaaaaaatg actcatccgc tggcgaactt
    12421 agcataataa tgcctttaat cattttctgc ttaattttgc tttcgcattt ttgtaagtct
    12481 tctgccaggt tgtgcgacgt ctgcaatatc acgtcgaaac cttcttcttc agctttggcg
    12541 gtgatggcat gtaaaacttc cgagaaaaac ggattacccg ccgtagtttt ggtcgaacgg
    12601 gtagaaatca ccataatggc atcaaaaccc gaagaggtca gcgcgcgtgc cagcttgttt
    12661 ggttgatact gtaattcttc aatggcccgt aacacttttt cgcgcgcttc cggggagata
    12721 ttggtttgct tattcagcac gcgtgatacg gtggattttg atacgcctgc aaccctggca
    12781 atatcataaa tagtagggga cataggtcag gaactccgtc cgaacgtgaa tgttgcacat
    12841 cttatggagt tcgcccgtcg atgacaacac gggcagttga taatcaatgg cctggccccc
    12901 acattcatat ccttactgat ggttttttct ggtccacagg acagcaatga agcgacttaa
    12961 aaatgaactt aatgcgctgg tgaatcgggg tgtcgacaga catctgcgcc tcgccgtaac
    13021 cggacttagc cgcagcggca aaacggcgtt tatcactgcg atggtcaatc agttgctcaa
    13081 tattcacgcc ggagcaaggt tgccgctgtt aagcgcggtg cgtgaagaac ggctgctggg
    13141 cgtaaaacgc attccccagc gtgactttgg cattccgcgc ttcacctatg atgaagggct
    13201 ggcgcagtta tatggcgatc cacccgcctg gccaacgcca acgcgcggcg tcagcgaaat
    13261 tcgcctggcg ctacgtttta aatcgaacga ttcgctgcta cgccacttta aggatacctc
    13321 cacgctgtat ctggagattg tggattatcc cggcgaatgg ttgctcgacc tgccgatgct
    13381 ggcgcaggac tatttaagct ggtcgcgcca gatgacgggc ttacttaatg gtcagcgcgg
    13441 cgaatggtcg gcgaaatggc gaatgatgtg cgaagggctg gacccgctag cgcctgccga
    13501 cgaaaaccgg ctggcagaca ttgccgccgc ctggaccgag tatctccacc actgcaaaca
    13561 gcaggggttg cactttattc agcctgggcg ctttgtcttg ccgggagata tggcaggagc
    13621 acccgcgctg caattcttcc cgtggccgga tgtcgatgcc tggggcgagt ccaaactggc
    13681 gcaggccgat aagcatacca atgccggaat gctgcgcgag cggtttaatt attactgcga
    13741 gaaagtggtg aaggggttct ataagaatca ttttctgcgc tttgaccgcc agattgtgct
    13801 ggtggattgc ctgcaacctc tcaacagtgg gccacaggca tttaatgata tgcgtctggc
    13861 gctgacgcag ctgatgcaaa gtttccacta cgggcagcga accctgttcc ggcgtttgtt
    13921 ttcaccggtt atcgataagc tattgtttgc tgccactaaa gcagaccatg tgaccatcga
    13981 tcagcacgcc aatatggttt cattactgca acaactgatt caggatgcct ggcaaaatgc
    14041 ggcgttcgaa gggatcagca tggactgcct ggggctggcg tcagttcagg cgaccaccag
    14101 cggcattatt gatgttaacg gtgagaaaat cccggcgctg cgcggtaatc gactcagcga
    14161 tggcgcaccg ctcactgttt atcctggcga agttcccgct cgtttgcccg gtcaggcgtt
    14221 ctgggataag caaggcttcc agtttgaggc gtttcgtccg caggtgatgg atgttgacaa
    14281 accgctgccg catattcgcc ttgatgctgc gctggaattt ttaataggag ataaattgcg
    14341 atgaccgaac cgttaaaacc acgtattgat ttcgacggtc cgctggatgt cgatcagaat
    14401 cctgaattca gggcgcagca gacctttgac gaaaatcagg cacaaaattt tgccccggcc
    14461 acactcgacg aagcgcctga agaagagggg caagttgaag cggtaatgga tgcagcgtta
    14521 cgtcctaaac gcagcctgtg gcgcaaaatg gtgatgggcg ggctggctct gtttggcgca
    14581 agcgttgtcg ggcagggtgt acagtggaca atgaatgcct ggcaaaccca ggactgggta
    14641 gcgctgggtg gatgtgctgc aggggcattg attatcggcg ctggcgtagg ttctgtggta
    14701 acagagtggc ggcgcttatg gcgcttgcga cagcgcgccc atgaacgcga cgaagcgcgt
    14761 gatttattgc acagccacgg caccggcaaa ggccgcgcat tttgcgaaaa actggcacag
    14821 caggcgggca tcgatcagtc acatccggcg ctgcaacgct ggtatgcctc aatccatgaa
    14881 acgcaaaacg accgtgaagt ggtcagtttg tatgcgcatt tggtccagcc agttttagat
    14941 gcccaggcgc ggcgtgaaat cagccgttcg gcagcggaat caacattgat gattgcggtc
    15001 agcccgctgg cgctggtgga tatggcattt atcgcctggc gcaatctgcg tttgattaat
    15061 cgcatcgcca cgctgtatgg cattgaactg gggtattaca gccgtttgcg cctgtttaag
    15121 ctggtattgc tgaatatcgc tttcgccgga gccagcgaac tggtgcgcga agtggggatg
    15181 gactggatgt cgcaagatct cgctgctcgt ttgtctaccc gcgcagctca ggggattggt
    15241 gcaggacttc tgacggcacg actggggatt aaagctatgg agctttgccg cccgctgccg
    15301 tggcttgacg atgacaagcc acgcctcggg gatttccgtc gtcagcttat cggtcaggtg
    15361 aaagaaactc tgcaaaaagg caaaacgccc agcgaaaaat aatgcaatat cgggtgctga
    15421 ccggatatct ttacgccgaa gtgcccgttt ttccgtcttt gtgtcaatga ttgttgacag
    15481 aaaccttcct gctattcaaa tagtgtcata tcatcatatt aattgttctt ttttcaggtg
    15541 aaggttccca tgcgtctgga agtcttttgt gaagaccgac tcggtctgac ccgcgaatta
    15601 ctcgatctac tcgtgctaag aggcattgat ttacgcggta ttgagattga tcccattggg
    15661 cgaatctacc tcaattttgc tgaactggag tttgagagtt tcagcagtct gatggccgaa
    15721 atacgccgta ttgcaggtgt taccgatgtg cgtactgtcc cgtggatgcc ttccgaacgt
    15781 gagcatctgg cgttgagcgc gttactggag gcgttgcctg aacctgtgct ctctgtcgat
    15841 atgaaaagca aagtggatat ggcgaacccg gcgagctgtc agctttttgg gcaaaaattg
    15901 gatcgcctgc gcaaccatac cgccgcgcaa ttgattaacg gctttaattt tttacgctgg
    15961 ctggaaagcg aaccgcaaga ttcgcataac gagcatgtcg ttattaatgg gcagaatttc
    16021 ctgatggaga ttacgcctgt ttatcttcag gatgaaaatg atcaacacgt cctgaccggt
    16081 gcggtggtga tgttgcgatc aacgattcgt atgggccgtc agttgcaaaa tgtcgctgcc
    16141 caggacgtca gcgccttcag tcaaattgtc gctgtcagcc cgaaaatgaa gcatgttgtc
    16201 gaacaggcgc agaaactggc gatgctaagc gcgccactgc tgattacggg tgataccggc
    16261 accggaaaag atctctttgc ctacgcctgt catcaggcaa gccccagagc gagcaaacct
    16321 tacctggcgc tgaactgtgc gtctataccg gaagatgcgg tcgagagtga actgtttggt
    16381 catgctccgg aagggaagaa agggttcttt gagcaggcga acggtggttc ggtgctgttg
    16441 gatgaaatag gggaaatgtc accacggatg caggcgaaat tactgcgttt ccttaatgat
    16501 ggcaccttcc gtcgggttgg cgaagaccat gaggtgcatg tcgatgtgcg ggtgatttgt
    16561 gctacgcaga agaatctggt cgaactggtg caaaaaggcg tgttccgtga agatctctat
    16621 tatcgtctga acgtgttgac gctcaatctg ccgccgctac gtgactgtcc gcaggacatc
    16681 atgccgttaa ccgagctgtt cgtcgcccgc tttgccgacg agcagggcgt gccgcgtccg
    16741 aaactggccg ctgatctgaa tactgtactt acgcgttatg cgtggccggg aaatgtgcgg
    16801 cagttaaaga acgctatcta tcgtgcactg acacaactgg acggttatga gctgcgtcca
    16861 caggatattt tgttgccgga ttatgacgcc gcaacggtag ccgtgggcga agatgcgatg
    16921 gaaggttcgc tggacgaaat caccagccgt tttgaacgct cggtattaac ccagctttat
    16981 cgcaattatc ccagcacgcg caaactggca aaacgtctcg gcgtttcaca taccgcgatt
    17041 gccaataagt tgcgggaata tggtctgagt cagaagaaga acgaagagta agcgtgaata
    17101 tgcctgatgg tgcaacacca tcaggcatat taacttatgc tttcagtaca gccagagctg
    17161 cttcgtaatc cggctcggtg gtgatttcat ccaccagctg gctgaaaatc acattgtcat
    17221 tttcatcaat aaccacaacg gcacgcgctg ccagaccttt cagtgggcca tcagcaattg
    17281 ccacaccgta ggcttgcagg aattcagcgt tacggaaagt ggagagggtg ataacgttgt
    17341 tcagaccttc tgcgccgcag aaacgagact gggcgaacgg cagatcggca gagatacaca
    17401 gcacaacggt gttgtcgatc tcggttgcca gttggttaaa cttacgtact gatgcggcgc
    17461 aaacaccggt atcaatactc gggaaaatgt tcagcacttt gcgtttaccc gcaaactgac
    17521 cgagggtgac gtcagacaga tcttttgcca cgagagtaaa agtctgcgct ttgctacccg
    17581 cctgcgggat ggaattggcg actgtaaccg ggttgccctg gaaatgaacg gtttgtgaca
    17641 tgattatctt tcctgtttac atatagttaa cgtcacacct agtttatgct aactgtcaat
    17701 aacacagcaa acgctatttg cgcttaatcc gcagaccacc gcgacaacaa ggagtaaaga
    17761 tgagaaccgt taaggtattt gaggaggcct ggcccttaca taccccgttt gtgattgctc
    17821 ggggaagtcg cagtgaagcg cgcgtggtgg tggttgaact ggaagaagaa ggtattaaag
    17881 gcaccggtga atgcacgccg tatccgcgtt atggagaaag tgatgcctcg gtaatggcgc
    17941 aaattatgag cgtcgtgccg caattagaga aagggctgac acgggaggag ttgcaaaaaa
    18001 tcctccctgc cggcgcagca cgtaatgcgc tggattgtgc attgtgggat ctggcggcgc
    18061 gaaaacagca gcaatcgctg gctgctttga tcggcataac gcttcccgag acagtcacca
    18121 cggcacagac gatagtcatc ggtacgcctg atcagatggc caatagtgca tcaacactct
    18181 ggcaggcagg cgcgaaatta ctgaaagtga agctggataa ccatcttatc agtgagcgga
    18241 tggtggcaat tcgcaccgct gtgcccgatg cgacgctgat cgttgatgca aatgaatcct
    18301 ggcgtgcaga agggttggcg gcgcgttgcc agctattggc agatttaggc gttgcgatgc
    18361 ttgaacaacc gctttctgcg caggacgatg cggcgctgga gaattttatt catccgttac
    18421 cgatttgtgc tgatgaaagt tgtcatactc gtagcaatct gaaggcgctg aaagggcgct
    18481 atgagatggt taacattaag cttgataaaa ccggtggtct gacggaagcg ctggcgctgg
    18541 cgactgaagc gcgtgcacaa ggttttcgtc tgatgctggg ctgcatgttg tgtacctctc
    18601 gtgccattag cgccgcttta ccgctggtgc cgcaggtcag tttcgccgat cttgacggac
    18661 cgacctggct ggcggtagat gtggaaccgg cgcttcagtt cacgacgggc gaattgcatc
    18721 tttaggatgc cagcgcagca agtttgccat cgcaaagaga tatttttcgc tggcttcgtc
    18781 ggaggagatg ggtggaaact ctgcggtgat gcaatgcaag ttgagatctg cacaccaact
    18841 gccgaaagag ccaggtgttt cataaccgac gctggttacc agaggcaatt caaacgcctg
    18901 ggccagccat tcacctaatt cgctgtgtct gggatcttca atacaggcca gcggatcgtg
    18961 gaatgagacc acccaggcag gttgaatgcg gtgtataagt tggcacagcg cctgggtttc
    19021 aggttcggag ccaggtttgc cacctgtcag taaaacgaca tcacgttctt cagcggcgct
    19081 gttccagcga taaactgttt caccttcctt ccagttcgct gccggaaagt ttcggtttaa
    19141 atccacacca ttagcattgg cccgtaaccc taactggcag ccgtcaggat tcacacacag
    19201 caccacatga tggcggcgca aagacggtgt caaagtccgc agagcacagg agagcgtgac
    19261 gatcgaagag ttttcatcac cgtgagtgcc agccagaatc aaaccacttt cgtgactggc
    19321 tgcaggggcc ggaaaccaga ttaacggcgc gcccaataat gaacgtccgt aatgttctgt
    19381 accgggcgga aatgcgccgc gttcggcgcg tgggcgagtt acggtcatga tcgtctctgg
    19441 ttcaggaagt attacaggca gtgttgtgca aattttgcgg attatcaaat ccttttcttc
    19501 aacgctatgt tatcgatctt gtatcacttt tttgatggaa gttgtttgta ttttcattga
    19561 tgtgcagata ttagaattgt cgtaatgtta tatacatggc gtattttatt cttactggtt
    19621 ctacacatgt taatcgcatc tgattgatca gttcacctta ataagtttaa taaatcgtac
    19681 tgcacatagt acagtacgat ggtagctata taattgattt acatgaagaa gggcttatca
    19741 ccatcagtcc cgggttgatt gatgcctata ttctcaccaa tgtgtttctc aggtttatcg
    19801 atgatatctg tgattaatgc tgcgacactc ttacgtgata cgatcgtacc tttgaatggt
    19861 tcattgcgtg aggtaagctc ataatcaata atatcttcat cagtcagcca ggcagggcga
    19921 agaattgtat attccagtcc agaagcctca attgcatccg cagccctgcg gaatggtttt
    19981 aggggttcac caataactgc attattccat tcgccaaatt ttcctggaac ttcatcataa
    20041 ataccgaggg aaagaacaaa gatcagacgt ttaacatcac aggctttcat cgcagcgatc
    20101 actgaatttg cctgaatatc taaatcttcc cccgtaaggt tggcatacac aacatcctgt
    20161 ccctgcatgg cctgcttcag tgctgcatga ttcagtacat cccccataat aatcttgcta
    20221 ttagttggat agggtttgtg gatttttgct ggctgacggg caaataaagt ttgtttaatg
    20281 gtttgtttat ctgccagttg attaatcaca tggcgggcta tctgaccgcc agcaccaaga
    20341 atcagtatat ttttcatgaa acttttccca gattattggc agcaatatgc caggccatat
    20401 cactattacg gatttgaata tgtttcatta cagagtttcc tcaaagaacg gagctaatac
    20461 tgaaattgct tcagccacat agactttgcc gtcataaaga tccatatggt ttgcgccttc
    20521 aacaatgtga tagcgtttat ccttgcttga tgctcgatcg tacaggtcgt cactcatcca
    20581 tttgctccca gcctggctgc ccgccacaat ctgtatcggc tgagtcaggt acacttccgc
    20641 catatggtaa gcatcatagg taataatctg gttaaggctg cgcaaagtag cgtaacccgg
    20701 tgctgttgga tactgcgcgc gaggggtgtg gtaatactcc caggcctgac gcagttcttc
    20761 gttcggcgca tcggactcct tcattggtgc cagtggcata gtggcgtatt ctccgctgct
    20821 aatatcgctg gttcgggcgt ctgaacccgc gtcaacgtat ggtagggcat caatagattt
    20881 cacattgttt tcccaaccat tacggaacat cgaaccaata ttgaccgcac taacagtacc
    20941 gatggccttg atgcggcgat cctgaattgc agcattggct gtatatcctg ctccggcaca
    21001 aattcccatc gcaccaattc gggtattgtc gacataagaa agcgttgtca ggtaatcaat
    21061 cacggcactg acgtcttcag tacgaatgta tgggttttct aactgacgcg gctcgccgcc
    21121 gctttcacct tgataagatg cgtcataagc aatagtgaca aatccctttt ccgccagttt
    21181 ttcggcatag gttccggccg tttgctcttt aacgccccca cctgggtgag ataccacaat
    21241 taccggatac tgacgggttt catcaaattt tggagggaaa tagatcactg cagacaaaga
    21301 gatggttgga ttattgctgt tagtgaagct gactttatta ttcatcattc cgttcctcat
    21361 ggagttgtcg gttcgtttta acggttggtg atatcactat agatattgat cattaagttg
    21421 attagaccca aaatcatgat tagacctatc atttaaatga ttaatagatt gtatggagat
    21481 actgaatgaa gcgtgaagaa atcgctgatc tgatggcgtt tgtcgttgtt gcagaggagc
    21541 gtagcttcac tcgcgcagca gcccgcctga gcatggcgca gtcagcttta agccagatag
    21601 tgcgccgtat agaagaacga ttgggattgc ggcttctgac gcgaaccacg cgcagcgttg
    21661 tacccactga agcgggcgag catcttttgt ctgttcttgg ccctatgttg catgacatag
    21721 attctgccct gacatccctg agcgatctgc agaaccgccc atccgggaca atacgtatta
    21781 ctactgtaga acatgcagca aaaacgatat tgttaccagc tatgcgcaca ttcctgaaat
    21841 cgcatcctga aattgatatt cagcttacca ttgattatgg tttgaccgat gtcgtttctg
    21901 aacgttttga tgcaggcgtc cgtctgggtg gggagatgga taaagatatg atcgccattc
    21961 gaatcgggcc agacatacca atggctattg ttggctcacc ggattatttt tctcgtcgaa
    22021 gtgctccaac gtcagtgtca caattaatag atcatcaggc aattaatttg tatcttccca
    22081 catcgggtac agcaaatcgc tggagattaa tacgcggtgg acgtgaagtt cgtgttcgca
    22141 tggaaggtca gcttttactg aatacgatag acctgatcat tgatgctgca attgatgggc
    22201 atggattggc gtatctacct tatgatcagg ttgagcgggc tattaaagaa aaaaaactga
    22261 tacgtgtttt ggataaattc acaccagatt tacccggtta tcacctgtac tatccacacc
    22321 gtcgacatgc tggctcggca ttctcattat ttatagatag gctgaagtat aaaggtgctg
    22381 tttagcacta cttgctgata cattaattta attcttctct taacgtattc tcagttcctt
    22441 tcaacgtttt ggttattttt tattcttcgc acaatggcga cagatgctga ttatgatacc
    22501 gaaaacgggt ttgaacgtgc gaagcccgaa cgtagtgttc ggagttctat gtgctttacc
    22561 gcattttgga gactattatt tacactaaat ctgatttgat atattgatac ttaaaacatt
    22621 tgatgcttcc tttgtcactt ttttgatgga agttgtttgc atttctttaa ggcgaaacaa
    22681 ataattacgc atcaatttta atgtcggtta gagggaaact tatgaagcac tctgtttcag
    22741 tcacgtgttg tgtgctgttg gtcagcagca tttctctttc gtatgctgct gaagttccga
    22801 gcggcacagt actggcagag aagcaggagc tggtgcgcca cattaaagat gagccagcgt
    22861 cgctggatcc cgctaaagcc gtgggcctgc cggagattca ggtcattcgc gatctgtttg
    22921 aaggactggt gaatcagaac gagaaagggg agattgtccc cggcgttgcg actcagtgga
    22981 aaagtaatga cagccgtatc tggactttta ccctgcgcga taacgcaaaa tgggcggatg
    23041 gcacaccggt aacggcgcaa gattttgtct acagctggca acgtctggtg gacccgaaaa
    23101 cgctatcgcc atttgcatgg tttgccgcgc tggcgggaat caacaacgca caggcgatta
    23161 ttgatggtaa agccacgcct gaccagcttg gcgtcaccgc agttgatgcc catactttga
    23221 aaatccagct tgataaacca ttgccgtggt ttgtgaattt aaccgctaac tttgccttct
    23281 tcccggtgca aaaagccaac gtagaaagca gtaaagagtg gacgaaaccc ggaaatctga
    23341 tcggcaatgg cgcttatgtt cttaaagagc gcgtagtcaa tgaaaaactg gtcgtagtgc
    23401 cgaataccca ttattgggat aacgccaaaa cggtactgca aaaagtgacc ttcctgccaa
    23461 ttaatcagga atccgcagcc actaagcgtt atctcgcggg ggatattgat atcaccgaat
    23521 ccttcccgaa aaatatgtat cagaagttgt tgaaggatat tccggggcag gtttatacgc
    23581 cgccgcagct cgggacctat tattatgcgt ttaacacgca aaaagggccg acggcagatc
    23641 agcgcgttcg cctggcatta agtatgacga tagatcgccg cctgatgacc gaaaaagtgt
    23701 tggggacggg cgaaaagcca gcgtggcatt ttacaccaga tgttaccgcg ggatttacgc
    23761 cggaaccttc gccgtttgaa caaatgagtc aggaagaact gaatgcgcag gcaaaaactt
    23821 tgttgagcgc agctggttat ggtccgcaaa aaccgctgaa gttgacgctt ttgtataaca
    23881 cttcagaaaa ccatcaaaaa attgcgattg ctgtagcatc gatgtggaaa aagaacctgg
    23941 gcgtagatgt taaattgcaa aaccaggaat ggaaaaccta tatcgatagc cgtaataccg
    24001 gcaattttga tgttatccgc gcctcgtggg taggggatta taatgacccc tccactttcc
    24061 tgacattatt aacgtcaacg cattccggga atatttcacg ctttaacaat ccggcatatg
    24121 acaaagttct ggcccaggca tcgacggaaa ataccgttaa agcgcgtaat gccgattaca
    24181 acgcggcaga aaaaatcctc atggagcaag caccgattgc accaatttat caatatacca
    24241 atggacgatt aatcaagccg tggctgaaag gttatcccat taataaccct gaagatgtgg
    24301 cgtacagtcg gactatgtat attgtgaagc attgatgtga tgggaactgg cgttaccctt
    24361 gtggataacg ccagtgatgt ctgatttagc gaccctggtc cggcggtgta atgttatcca
    24421 tatacagcgt ctggctgggg aaggcaaagt ccgcaccgtg tgactgtaca atatcgataa
    24481 tcttcaaata aacgtcttgc tgtgcagcaa gccactcagc ccataccgtg gttttggtaa
    24541 agcaataaac cataatattt aatgacgaat cagcaaactg gttgaaataa accagtaagg
    24601 tttgtcgctg gtcgatggcc gggtgatttt tcagcatctc acgtacggct tcgacaataa
    24661 cgcccacttt tgccgcatcc tcataacgta aaccaatggt cgtggtaatg cgccggttgg
    24721 tcattcgtcc aggattttct acgctgatcg acgaaaacag cgagttcggt acgtacaatg
    24781 gacgattatc aaaggtctta attttggtaa ttcgccagcc aatttccgct actgtacctt
    24841 cgatatttct gtccggtgaa cggatccagt cgccaatact gaaaggacgg tcgaaataga
    24901 gcataatccc ggaaaagaag ttactcagaa tatctttacc ggccataccg acagccagac
    24961 caccaatacc accaaaggtc aacaagccag aaaggctcat gccgaaatgt tcgccataaa
    25021 gcagaacaag caccacaata atggtgattt tgataatgcg cgacatgatc cgcgcactgg
    25081 tgatatcgcg acctttttta atctgctgtt tttcaaactg attaatcagc agaaatagct
    25141 taatcgtcag aataaccgca atcagggacg tacagataaa atcgataacg cctggggtga
    25201 taaatttgag tttatagttt tctataacat agttaatgat gctaccaaca gcactaataa
    25261 ttatggtgta gattaaaaat tgcaccgcat ggaataaaaa tccttttctt ttacgatttc
    25321 cacggcgaaa ccaaaagctc atcagaatca atgctgcgca gctaccgaaa ataatgacca
    25381 gattaagcgc attatttgta aacagttcag cgatcattgt tttatcaggc tcctccagat
    25441 aattgtcgtc atgccggaaa cccctggcgg ggctatttta ccgcgacaat tcattcagat
    25501 catcaatagt cagggaagta agtagcaaca ttagctaaca aatcaatgca ttgattgtaa
    25561 atccagctaa gaggtgaggt tttcagagca gacaagagtg aaatgtcatg gtattgttac
    25621 gtttaggtaa caagaaattt gtctgcacaa ggattacatc atgattatgg cgaaactgaa
    25681 gtcagcgaaa gggaagaaat ttctctttgg tttgttggcg gttttcatta ttgcggcgtc
    25741 ggttgtgact cgcgcgacca tcggcggcgt tatagaacag tacaatattc cgctgtctga
    25801 gtggacgaca tcaatgtatg tgattcagtc atcgatgatt tttgtttata gcctggtctt
    25861 tactgtgttg ctggcaatcc cgttgggaat ttatttcctt ggcggcgaag agcagtaagt
    25921 aaaaaatagg cccgataact cgggccttgt cagttattga agagtcgtta atcgtcttct
    25981 tcgtcatcca gttcaacggg tgtctgatac tggtcaggtt taatgaccag caggtcgcag
    26041 cgaagatgat caatcacctg ttccgccgtg ttgccaagga atgctgctga aataccggtg
    26101 cgtccgaccg tgcccagaac cacaatcccc gcctgtaaat gctccgccaa atcaggaatc
    26161 acctcttctg gcagaccttt ttctacgtgc gtcatgtttt cattaatgcc gaatttctgc
    26221 cgcagggctt tcattgccag caaatgttgc ccacgaatgg catcgttata aacgctcgga
    26281 tcaaattccg gcagttcaat cgcgatatta attggcgtta ccggataagc gccaaccaga
    26341 tgaacttcgg tatggttgac ttgttccgcc agttcgatcg tctctttgac cagtttttca
    26401 ttgagcgcat tatggtacgg ttcttcactg gcgagattca ccgccaccag cgccttaccc
    26461 ccttccggcc acggctggtc tttcaccatc cacacggggc ttgggcattt gcgtaacaga
    26521 tgccagtccg ttggcgtaaa aatcaccgct tccagacggt catgttggtg cgccattttt
    26581 agcaccaaat cgtgcccgcc gctgatcact tcctgaatga tggcttcgaa aggacggtta
    26641 tgccagacca ctttaatttc aatgggaacg ccagcattga gataatattt tgcctgctcg
    26701 tggatccagg ctgtacgctg gctgatgacg ccctgacgca tagcggtacg ttcgtccgga
    26761 gagagcaggg tggtcatttc gtatgagaag tcatagatcg gcaaaaaggc tttaattttg
    26821 ccaccaatcc gttgatgtaa ataaacagct cgccgcaatg ctggttggtc gtcctggtta
    26881 ggatcgataa caacgagcat gttctgatac atagccatac agggtctcct tacaacaact
    26941 gtcaacgcag tttgtaatta aaagattaac ccatatctgg tgaatgaaac agtgatgaac
    27001 cttctgtcag atcaataaat cagaaaaatt taatgatatg acagaaggat agtgagttat
    27061 gcggaagaat caggcaacgt tacgcgtgtg accagcaagc tgggccagcg catcgttatt
    27121 ttcgatggtg atgtatttac ctttgactgc cagcataccg cttttctgga agcggcccag
    27181 cagacggctg atggtttcaa ccgtcaggcc cagatagtta ccgatatcac cacgagtcat
    27241 cgtcaggcgg aattcacgag gggagaagcc gcgttgggca aaacgacggg acaggttgta
    27301 gatgaatgca gccagacgtt cctcggcatt tttcttcgac aacagcagga tcatgtcctg
    27361 atcgcctttg atttcaccgc tcatcagacg catcatctgc tgacgcagat tcggcatttt
    27421 accggacaaa tcgtccagcg tttcgaacgg gatttcacat accatcgagg tttccagcgc
    27481 ctgagcgaag ctcgggtgat gaccgctgcc gatagcgtca aatcccacca ggtcgcctgc
    27541 taaatggaaa ccagtgattt gctcgtcgcc ttgctcagtg atggtataac ttttaatcgt
    27601 accggagcgg atggcataaa gcgatttaag ttcatcacca gccttaaaca gcgtctggcc
    27661 tttctgaata ggcttcttcc gctcaatgat attatcaagc tgatcaagct catgttcgtt
    27721 gagtgtgaac gggatgcaaa gctggctgat gctgcaatcc tggcaatgga tagcacaacc
    27781 gccagactga atgcgccgta taattcgctt ttccgggatc ataggtctgc tcaagccgta
    27841 attgatattt gtcaatttta acatcttttt agggagcaag taagtctaag caaaccttaa
    27901 cagcagagaa ttccgatatt agatgtaaat atatgtctat ctatttgaaa acccttaagt
    27961 tgttaagggt aactttacat aaaagtgtga acaagctggc acaaattgtt taatgtttac
    28021 agcaaaagat aaccttcgtg gcgcaataac cactcttttc gctgaactcc gcctgcatat
    28081 ccggtcatgg taccgtttct gccaatgacc cgatgacaag gcacaacaat gctgatggga
    28141 ttcgatccat ttgccgcacc tacggcacgc gccgcaccgg ggcggcccaa ttgttcagcc
    28201 agttggccgt aatgcattac ctgcccgcag gggattgtgc gtaatgtttt ccagacttcg
    28261 cgctgaaatg gcgtcccccc cgtggcagtg ggaagcgtat caataatgct aagattaccg
    28321 gcaaaatatt cacgaagctt gtcgcttaaa ccacctgggt tggtggcaga aatgcgctca
    28381 tagccttctt tgcgataatg gatgtccagc agctgcacca tgcgttcgct gtactcttcc
    28441 cattcaaccg cccgcaggcg aaattgctca tcgcaaatca cccacagtgg acccagtggc
    28501 gtgacaattt tttcttcaag taatctcagc atccgttctc tcttaagaca aacgtgggta
    28561 aatccccgga cctatcggca gaccaacaag ataccacgcc agcaacatca gcagccatac
    28621 caccaaaaag ataagtggat agggcaaaac taacgaatag taagtaccca gtttcgcgtc
    28681 tggtttgtag cgttgcagga atccaagaaa cagtggaaca aaaggagata ctggcgctaa
    28741 aggcaatacg gatgagtcgg caatacgaaa gaggatttgc gcaaatgccg ggtgaaagcc
    28801 aagtagcata aacatcggta cgaaaatggg ggccagaatc gaccagattg cggaaccgct
    28861 ggcgataaac atgcataaga aagaggaaag caacgccaga ccgacaaacg ccgggatgcc
    28921 gctaagccct gaactttcca gtatatcggt cagccccacg gcgatgaatt tccccatgtt
    28981 gctccagtta aacatggcga caaactgggc gaggggaaaa accatcacga taaatcccgc
    29041 catctctttc atcggttcaa tcattaaatg cggtaaatcc gcctgacgtc gaattgtgcg
    29101 ggtagcgatg ccataagcca gcgagaccac aaagaaaaaa agaatgatca gtggcacgat
    29161 acctttaata aagggtgatg gcatcacggt gtgattaatc ggatcgcgca atatcccgtt
    29221 ttccgggatc accatcagcg caatcgcagc aataaaaagt agcgatacga cacctgctat
    29281 gcgtaaacca aaacgctgac tttcggtcaa tgtctgcagt ttctcatcgc tgtttccctg
    29341 ccattgacct aaacgtggct caatgatttt gtcggttatc aggccgccaa caatcgtcag
    29401 tacgactacg gagctggcca taaaatacca gttatcaatt acactgacgt gcatttgcgg
    29461 attgaacgca gccgcagcct ccgtgctgat ccccgacagc aacacgtcgg ttgtgacaat
    29521 cagtaaatta gccgtaaagc cgcaacctac gcctgcaatg gcagccagta aacctgcaac
    29581 tggatgcctg cccaccgcca gaaaaattag cgcacccatc ggtggcatga tcactaacgc
    29641 cgcatcggaa gaaatgtggc tgaaaaaagc aataaacagc accatataac tggcgtagcg
    29701 ggcattaaca tgcgatgcca ttttaaccat tagcgctggc agtaagccta cgcgctccgc
    29761 cagaccggca cctaaaacca gcgccaggat cgcaccaagt ggagcaaaac cgctaaagtt
    29821 tttaataaca ttgggtaaaa accagtgtaa tccttccaca ctgagcaggt ttttcaccac
    29881 caccggcgta ccatcggccg ggtttttcgc actgacgcca aaggctgaca aaattgccgt
    29941 cgtcaccatg agtacgataa tcaaatagat aaagagcaaa aaaggatggg gaaccttgtt
    30001 accaattctt tcgacccagc catagagctt cccggattgg gaggacgacg gtatggatga
    30061 catactcatg ggcattcctc gtttgttgtg tttgcgttgt tgttgttatt ttaaaggtga
    30121 cggtgtcacg tttttcggga tagggcagtg atacggttgc gtgtccgtaa cttgctgatg
    30181 ctcttgttgg cattcttgca atagccctga atcaatgaag agattaagtg tggttgctgc
    30241 catagttttc gccgccagca gcattccttt atgagcaata gatgttcgcc cctggctaac
    30301 cagttgccac gtatgtagcg gggtaccgac ggcaaagcag gggctgaaac actgggcaac
    30361 aggcagtttc caactgacgt cgccgacatc agtcgatgcc gcaagcacgt tatcggtggc
    30421 ggcatatgga gcgacttcat tcgccagtac cgtttcacga tgacgtagtg caaaagcctt
    30481 gccgttttcg ccaccggttg cagcgatatt attcagactg ttttgccgat cgttgggggt
    30541 gagcgtagcc tgaatttgtt tcgcaaaagc cagttcttcg cagttccatt ccggggtacc
    30601 aaaatgggat agggctcggt acatggcatt ttctaaggtg cgattcggga gataactgga
    30661 acaggctttg tcgaagcggc attcaaccgt ggtttcggtc atcaatgccg caccttcggc
    30721 gattttggcg acccgatcat aaatatgctg cacatcggtc atttcggggg cgcggataag
    30781 ataaagcact tctgcctgcg cctggaccac gttgggcgag atcccgccgc tatctgtgat
    30841 ggcatagtgt acgcgcgctt tttcaataat atgttcgttg aggaagttgg tgccagtggt
    30901 catcaacgtt acggcatcaa gggcgctgcg tcccaaatga ggggaattcg cggcatgtgc
    30961 tgcgatccct ttaaagcgcc atgatgcctg aatgtttgcc agcgtgcggg tattgaacat
    31021 accggcaaag gcttccgggt gccaggtgag tgccgcatcc acatcatcaa ataccccctc
    31081 gcggaccatg aacgttttac ccgagccgcc ttcttcgcca ggacaaccat aaaagcgcac
    31141 cgtgccgcct tgcccatatt gttccagcca tttcttgacg gctattgcag cggcaaaggc
    31201 ggcggttccc agcaaattgt gtccgcaacc gtgaccattt tcaccgggcg tcgcggatgt
    31261 aggttgcgcg caacttgctt gctgacttaa acctgccagg gcgtcatatt cccccagcag
    31321 ggcgataacc ggtttgcctt gaccaaacga agcaataaag gcatttggga tattgcctac
    31381 gttgcgggta acggtgaagc ctgcagactc cagcgccgaa gccagatgct ccgctgacca
    31441 gaactcttca aaacgtgttt ctggatgatc ccagatttga tcggcaatat cggtataacg
    31501 ttggcgatcg gcttcaatcg catcgtcgat aaaacgatag atttcctgca tcagatacct
    31561 cgcgtccagg gaaaattgag cgcggtgcgc gccagcgttt cgacggcaat agcgagaacc
    31621 tgctcgtcaa aatcgaattt ttcattgtga tgacctgccg ccagctgtgt gccaaatacc
    31681 atgtaggagg cttgcccttg atgtcgctgc acgcgggcca tcattaatgt ggcatcttcg
    31741 gaacccgcag gcgcttcaac acgttcaatg gcctgattga cccccgcgac ctgagccgcc
    31801 tggatttgca accatgcgac ccattgcggc gaaggagaac tggcggtagc agcacccatc
    31861 agacgagttt caacgccgac accatacatg gttgctgcgc cctgaatcgc ttgttgtgca
    31921 cgttcaaaaa catattgatt aatgacgtcg ctggccccgc gtgtttccac tttcagcaac
    31981 gccgaggcag gaacaacatt acgaccgctt cctgcctgca taacgcccac gtttactctg
    32041 gaagcgcctt cgctgtgcgg ggcgattgca tgcagtgcaa gagttgcttg tgctgccgcc
    32101 aacaaggcat tgtgaccgtc ttctggtttt gcgcctgcgt gagcggcggt cccggtgaag
    32161 tgcgcgtcaa atttggtggt tgccataaaa ttatcactgc cgcacaccac ggtgcccgca
    32221 ggtacgccag tgccaatgtg cacggcagta aaataatcaa catcatctac gacacctgca
    32281 tcgaccatcg cccgcgcgcc acgcgtacct tcctctgcag gctgaaaaat cagtttgatg
    32341 acgccatgta gtccggactc gaattgttta agggtatgcg ccagcccaag cccaatggcg
    32401 gtatgcccat catgaccaca ggcatgcatc attccggcgt tacatgacgc aaaaccgtcg
    32461 cggtaggggc gatggctgac atcctgctct tcactgagat ccagcgcgtc catatcgaca
    32521 cggaaagcca tcaccggacc ggggcgacca gtatccaggg tagcgacgat gccagtgaaa
    32581 ccgccttcaa aagccgcaat ccattgttct agcgccccct gttgacgagc gcgctcgaat
    32641 tcgcgttgta gagtgaattc atcaggtaat cccatccggc tactttcatt aacgacttcg
    32701 cggcccagcg ccagtgaata accgagctgg tgcaattctt ccgcaacaac ggtggcagtg
    32761 cggaattcca cccagccaga ctctgcatag tggtgaaaat cacgtcgcca gtgcgataat
    32821 tttggggcaa gcgaattaat aaattgattc aaagactcca taacctttcc cgtcatcagt
    32881 aaaaagtgtg acccggttca cgtagcgata gtttttactt atcactaact gatttttcac
    32941 agttttaacc gttcataaat taccctgaca caatcatctg cattaaagta gatgccagtt
    33001 tctttggtct gataaataac ggttatcggt ggcgtcatgg cttttcaggt aaaaattcat
    33061 caaattcggg cttttgttga agtggctcgt cagggcagca ttcgcggagc gagccgaatg
    33121 ttgaatatgt cgcaaccggc actgagtaaa tctattcagg aactggaaga agggttggcg
    33181 gcacaactct tttttcgccg tagtaaaggc gtgacgttaa ctgatgccgg tgaaagtttt
    33241 tatcagcacg ccagtcttat tcttgaagag ctgcgcgcag cgcaagagga tattcgccaa
    33301 cgacaagggc aactggcagg gcaaattaat atcggcatgg gggccagtat ttcccgcagt
    33361 ctgatgccag ctgtcatatc tcgttttcat cagcagcatc cgcaggtaaa agtacgcatt
    33421 atggaagggc aactggtgtc gatgattaat gaattgcgcc agggagaact ggatttcacc
    33481 atcaatacct attatcaggg accctacgac cacgaattta cttttgagaa attactggaa
    33541 aagcaattcg cgatcttttg ccgcccggga catcccgcca ttggtgcccg ttcgatcaaa
    33601 cagttactgg actacagctg gacaatgccg acgccacacg gcagctacta caaacagttg
    33661 agtgaattgc ttgacgatca ggcgcaaacg ccacaggtcg gcgtagtctg cgagacgttc
    33721 tcagcctgta tcagtctggt ggcaaaaagc gattttctca gcatactacc tgaagaaatg
    33781 ggctgcgatc ccttgcacgg acaggggctg gtgatgttgc cggttagcga aattttaccg
    33841 aaagcgacct attatttgat tcagcggcgt gatagtcgcc agacaccact gaccgcgtca
    33901 ttaatcacgc aatttcggcg agaatgcggc tatctgcaaa gttaaaactg cataaaaaaa
    33961 atagagtctg tcgacatccg ccagactcta cagtacacac agcagtgcat ccgcgtctta
    34021 aatccggcat ggtctcctct gcgccggtga ctgtgttata acggttttaa cctttagttg
    34081 ccaattttct tcagtgacag atttcacgaa aatataagtt acattattaa tatagtgaat
    34141 gaataatcat gcataagcat tttgcttaaa atatcggcaa catttggaac ttattactgg
    34201 aaatttgggt aatacgttgt tggaccagcc cggtctggtt atcatatcgc gctcttaatt
    34261 gcgggaggat gtaacatgaa ccttgacgac aaatcgctgt ttcttgacgc catggaagat
    34321 gtccagccgc tgaaacgtgc tacagatgtc cactggcatc caacgcgtaa ccaacgtgcg
    34381 ccgcagcgta tcgacacgct gcaacttgat aatttcctca ccactggatt tctcgacatc
    34441 atcccgctaa ctcagccgct ggagtttcgg cgggaagggt tgcaacatgg ggtgctggat
    34501 aagctgcgca gtggtaaata cccgcaacag gcgagcctga atcttttgcg ccagccggtg
    34561 gaagagtgcc gcaaaatggt gttcagtttc attcaacaag ccctggcgga tggtttgcgt
    34621 aatgtgctga ttattcatgg taaagggcgg gaagataaat cgcatgccaa tattgtccgc
    34681 agctatgtgg cgcgctggtt gaccgaattt gatgatgtcc aggcatattg taccgcgcta
    34741 ccgcatcatg gcggcagcgg ggcgtgttac gtcgcactac gtaaaacggc acaggcaaag
    34801 caagaaaact gggagcgcca cgctaagcgc agtcgttgat ctcgagacgc atccgcggct
    34861 tatgccgcca gcacgcggtt gcgtccatca tttttcgccc gatacaaagc atcatcaacg
    34921 cgtttaaaca gctcatcgat gctttcattt ccttcgtgat gcgccacacc aatgctgacg
    34981 gtaaagcgtg gtaagcccga aatactcact tttgccacgc ttacgcggat agtttcagcc
    35041 agcgaaagcg cggtatccag tggggttctt ggtagcaata agacaaactc ttcgcctccc
    35101 caacgaaaca ccaaatcgcc tttgcgagcg caactttcga gggtgcgggc gagggcgcat
    35161 aacacctcat cacctttaga atgcccatag agatcgttaa tgtgtttaaa acgatcggtg
    35221 tcgatgagca acaagctgta atcctgagcg atggcgaggt gctgcatttg gcctggctcg
    35281 gtaatgtgat aaaactgtcg ccgattcagt aatccggtca tcgcgtcatg gtgggcagca
    35341 tgttccagct gctcctccag ccgtttttgc tcagtaatat catgcacaat acataacatg
    35401 agcttgtcgc cataaatttc aatcggaccg gcataggtct gcacatgacg agtcgaacca
    35461 tccgccaatt tatgaacaaa attcaaaggc ttatggccac cgggtaaatg cgaaatttca
    35521 tgcatgatag gcatgacgcg tcgcccgagc atatttattt cccaggtatg tttctggcac
    35581 atcgtttcat ggttataacc atagaaattg agcgcggcga ggttagcatc aacgatttgg
    35641 ccatctcgtg aggggtcaat caacaacatt ggtgcagagt tagtcagaaa aaagcgcgca
    35701 taaaaacctt gttttttgcg ctgataattt gccgagcgac tggcttttaa acccagcgtt
    35761 gccggcgctt cgataccttc gaaaataatc accggttctg cttctgtcag ctctcgcaaa
    35821 acaagccgac agcttaatgc tgtttcctct tctttacgtt gaacggtgag gatttcgata
    35881 atatcgtgtt ggttttgcag atcggagagg tatttcggca gttctttttg tgaggagacg
    35941 gaatagggac cggttcgtag ctgactaaac gtgaggtctt gcatcaacag tcgcgccgcg
    36001 ctgttggcat aaattaactg ttcctcaaag ggcgaaacga tccagacagg actggtgagt
    36061 aagtccaggg tattgaagtt gtgcgtaatc attgagatcc cgttattttt atcaattttt
    36121 gttgctatcc gatcgcaaaa aagccacgtc atatgatcag ataattctga taatgataga
    36181 cgctatttaa cacttcacac ggtttgtata cggaaaagca ttttgctttt tgtattcaat
    36241 ttagacagaa ttttattaat catttcaggg taatggggtg atgagatgtt gcgtaacagg
    36301 gccagaaggc tagactacaa aataatgcgt tgatgatgga ggcactgtgg aagcgattaa
    36361 gggatcggac gttaatgtcc cggatg
//
LOCUS       DAMZFT010000034        36329 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.034, whole genome shotgun sequence.
ACCESSION   DAMZFT010000034 DAMZFT010000000
VERSION     DAMZFT010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 36329)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36329)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36329
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.034"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..169
                     /locus_tag="PN977_001821"
     CDS             <1..169
                     /locus_tag="PN977_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015974252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage antirepressor KilAC domain-containing
                     protein"
                     /protein_id="HDI6041857.1"
                     /translation="RQQYINAGYFTVKEVVLDDENGYQIRLTPQLTGKGQQWLTRKLL
                     DAGLLKPVAIG"
     gene            334..1134
                     /locus_tag="PN977_001822"
     CDS             334..1134
                     /locus_tag="PN977_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001187871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor KilAC domain-containing
                     protein"
                     /protein_id="HDI6041858.1"
                     /translation="MQQTFNADMNISNLHQNVDPSTTLPVICGVEITTDRAGRYNLNA
                     LHRASGLGAHKAPAQWLRTLSAKQLIEELEKETMQNCIVSFEGRGGGTFAHELLAVEY
                     AGWISPAFRLKVNQTFIDYRAGRLQPAIPQSLPEALRLAADLAEQKQRLEQKMLMDAP
                     KVEFAERVATASGVLIGNYAKVLGLGQNYLFTWLRDNGILIATGERRNVPKQEYISRG
                     YFTLKETVIDTSNGSRISFTTRITGKGQQWLMKRLLDAGVLVPVAATR"
     gene            1164..2009
                     /locus_tag="PN977_001823"
     CDS             1164..2009
                     /locus_tag="PN977_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002431869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Replication protein repL"
                     /protein_id="HDI6041859.1"
                     /translation="MLAKVTFLSCITMSDFTFSGYELACFVTHSGLSRSAGHILSQCA
                     NLAATTSEYFIHKPHRLIAAETGYSQSTVVRAFREAVNKGILSVEIVIGDHRERRANL
                     YRFTPSFLAFAQQAKNALIESKLKISSAATKVKAVLAKTLALFNFLSTPPCQNDTPSP
                     CQDDVAIKNKKSQVKKTKRSVSGGAGTTRLKKLTSWIAEAKAKADNLRLSKKRAQKHE
                     FKQKVEAAARKYAYLKDKRSPDIGGISNFDNLPHCMTVNEALNAVLAKNKDNEQWGIP
                     AGFRG"
     gene            2060..2305
                     /locus_tag="PN977_001824"
     CDS             2060..2305
                     /locus_tag="PN977_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002433487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6041860.1"
                     /translation="MQSDFLQLAIAFAGYVCIGFCVYMISRKMLVDIDRKEQAEEILE
                     WIFFGAVWPLGIMFAATFLLMWIFTLPGDFYRKKARH"
     gene            complement(2488..3084)
                     /locus_tag="PN977_001825"
     CDS             complement(2488..3084)
                     /locus_tag="PN977_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000268403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6041861.1"
                     /translation="MAVIPMSYSPGTVARRFSILDGVTIQGVLYQVIWDSKTPFAAVI
                     EAAPSIIDGDMRHKVVATLELQRRPQLEGVLVRKFWEDSDVAQIEGIVVDGTVRDVGL
                     ATFVYETVASKAGVVLLSDNEQYEGGKALWQHIARRSSELKVFILDTDAAQYYPFDGE
                     RVCYDGVSIPESEIWSEHPDRSKHGVVLVAESIIGNAA"
     gene            complement(3256..3765)
                     /locus_tag="PN977_001826"
     CDS             complement(3256..3765)
                     /locus_tag="PN977_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000509944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate protein"
                     /protein_id="HDI6041862.1"
                     /translation="MGHNNTKGNRKFIKGRYTANAAKGERLVSSEFQLTFAGYEDISV
                     LVRTSQIPEMTREDVEDYGPNGVKFNQHGPIRNSGEIQVQCVETIEGDILQFIKDRIA
                     AKDYVDITMAATPESKSSGVNAVTKAATTIEMLDCKIYSDAIDFSTEDVTAAVRPSLR
                     IVYNWIEWD"
     gene            complement(3777..4358)
                     /locus_tag="PN977_001827"
     CDS             complement(3777..4358)
                     /locus_tag="PN977_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000035303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6041863.1"
                     /translation="MAPIAYGVYSQADGVSPYLKVTLTNSQYQVTGYISQGAAMNMAQ
                     NWEAPFTGMSMGSVSGALGGFVQVGTETTSVARWNSLMVWEGGTPPTFTLPVTFIALN
                     NPFIEVSGAIAALTAMISPELKAANVGGRIPERVTLNIGRRINITDVAIQDLSFDLDA
                     PRDSNGYFLKNTVNLQLTGSSIYNSSDIVRAFQ"
     gene            complement(4394..5209)
                     /locus_tag="PN977_001828"
     CDS             complement(4394..5209)
                     /locus_tag="PN977_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016236899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6041864.1"
                     /translation="MSKNFFQSGAFLGNGLSRFALNSDPVQLMESARASAEPPTDPVI
                     NNNPEPAAQTNDNVPSAPAPEQILEGKDGKEWTVEQAHQMILEAANRSAMQNALSDAA
                     DAVFSWADSGDLTFDSLDGFVQAIAGISDDDDSEVTEEQDDAYNEAWANVADFLAACG
                     VDDDLIEALADDEDDDAAADVGASIAGLDSDDRDELEAAFVVAGTSDEMLTEAFKKVV
                     RNGEIKLIRKRLRKKRLTAAQKSALKKARRKAQTGAAKLARKKSMKLRRKRLG"
     gene            complement(5219..6808)
                     /locus_tag="PN977_001829"
     CDS             complement(5219..6808)
                     /locus_tag="PN977_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6041865.1"
                     /translation="MSQYSIQQSLGNASGVAVSPINADATLSTGVALNSSLWAGIGVF
                     ARGKPFTVLAVTESNYEDVLGEPLKPSSGSQFEPIRHVYEAIQQTSGYVVRAVPDDAK
                     FPIIMFDESGEPAYSALPYGSEIELDSGEAFAIYVDDGDPCISPTRELTIETATADSA
                     GNERFLLKLTQTTSLGVVTTLETHTVSLAEEAKDDMGRLCYLPTALEARSKYLRAVVN
                     EELISTAKVTNKKSLAFTGGTNGDQSKISTAAYLRAVKVLNNAPYMYTAVLGLGCYDN
                     AAITALGNICSDRLIDGFFDVKPTLTYTEALSAVEDTGLLGTDYVSCAVYHFPFSCKD
                     KWTQSRVVFGLSGAAYAAKARGVKKNSDVGGWHYSPAGEERAVIARASIQPLYPEDTP
                     DEEAMVKGRLNKVSVGTSGQMIIDDALTCCTQDNYLHFQHVPSLMNAISRFFVQLARQ
                     MKHSPDGITAAGLTKGMTKLLDRFVASGALVAPRDPDADGTEPYVLKVTQAEFDKWEV
                     VWACCPTGVARRIQGVPLLIK"
     gene            complement(6869..8575)
                     /locus_tag="PN977_001830"
     CDS             complement(6869..8575)
                     /locus_tag="PN977_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000067705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6041866.1"
                     /translation="MSNLREYQNRIADIAKRSKAVLGWASTAQFGTDNQFIKDDAARA
                     ASILEAARKDPVFAGISDNATAQIATAWASALADYAAAHKSMPRPEILASCHQTLENC
                     LIESTRNSMDATNKAMLESVAAEMMSVSDGVMRLPLFLAMILPVQLGAATADACTFIP
                     VTRDQSDIYEVFNVAGSSFGSYAAGDVLDMQSVGVYSQLRRRYVLVASSDGTSKTATF
                     KMEDFEGQNVPIRKGRTNIYVNRIKSVVDNGSGSLLHSFTNAAGEQITVTCSLNYNIG
                     QIALSFSKAPDKGTEIAIETEINIEAAPELIPLINHEMKKYTLFPSQFVIAAEHTVQA
                     AYEAQREFGLDLGSLQFRTLKEYLSHEQDMLRLRIMIWRTLANDTFDIALPVNQSFDV
                     WATIIRGKFQTVYRDIIERVKSSGAMGMFAGADAASFFKQLPKDFFQPAEDYIQTPYV
                     HYIGTLFGNVKVYEVPAGICKNLTTENIQFSSMDVLCYVRDENPGKAGFVTGDAVPAI
                     PFQHPTTPALVNRTTLWGSAINDMHPRNGADYFTRVTLTMAKKGGLNFISGDTIDAGD
                     SE"
     gene            complement(8801..9802)
                     /locus_tag="PN977_001831"
     CDS             complement(8801..9802)
                     /locus_tag="PN977_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HDI6041867.1"
                     /translation="MSKKNRPTIGRTLNPSILSGFDSSSASGDRVEQVFKLSTGRQAT
                     FIEEVIPPNQVESDTFVDQHNNGRDQASLTPKSLKSIRSTIKHQQFYPAIGVRRATGK
                     IEILDGSRRRASAILENVGLRVLVTDQEISVQEAQNLAKDVQTALQHSIREIGLRLMR
                     MKNDGMSQKDIAAKEGLSQAKVTRALQAASAPEELVALFPVQSELTFSDYKTLCAVGD
                     EMGNKNLEFDQLIQNISPEINDILSIEEMAEDEVKNKILRLITKEASLLTDKGSKDKS
                     VVTELWKFEDKDRFARKRVKGRAFSYEFNRLSKELQEELDRMIGHILRKSLDKKPKP"
     gene            complement(9819..11015)
                     /locus_tag="PN977_001832"
     CDS             complement(9819..11015)
                     /locus_tag="PN977_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDI6041868.1"
                     /translation="MSDSSQLHKVAQRANRMLNVLTEQVQLQKDELHANEFYQVYAKA
                     ALAKLPLLTRANVDYAVSEMEEKGYVFDKRPAGSSMKYAMSIQNIIDIYEHRGVPKYR
                     DRYSEAYVIFISNLKGGVSKTVSTVSLAHAMRAHPHLLMEDLRILVIDLDPQSSATMF
                     LSHKHSIGIVNATSAQAMLQNVSREELLEEFIVPSVVPGVDVMPASIDDAFIASDWRE
                     LCNEHLPGQNIHAVLKENVIDKLKSDYDFILVDSGPHLDAFLKNALASANILFTPLPP
                     ATVDFHSSLKYVARLPELVKLISDEGCECQLATNIGFMSKLSNKADHKYCHSLAKEVF
                     GGDMLDVVLPRLDGFERCGESFDTVISANPATYVGSADALKNARIAAEDFAKAVFDRI
                     EFIRSN"
     gene            complement(11184..11993)
                     /locus_tag="PN977_001833"
     CDS             complement(11184..11993)
                     /locus_tag="PN977_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016246522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HDI6041869.1"
                     /translation="MQEEKQHYLYVLVPENGDTFKIGISCGPLARFKGLQVSPDFALS
                     RVYRGTRLAMVNLERALHATFFPWNAPWEKSVGGGHTEWFTRECLDKVLAHIEYLNDM
                     WGGILERIKSNDLLQRPVDAACSFEKELDVTSIVTFKDDAGMRDVAYVSISGYEPDAI
                     RAQCELLKAMFMLRTKYPWDTGRVCFPMEELTATIDSQLYHDNPEKFFSLLAGNGLNC
                     VSGLGRSRIQHASLFGPFFYDRHGYFEAELPALTRAIDTIDFERLFAALSK"
     gene            complement(12286..13170)
                     /locus_tag="PN977_001834"
     CDS             complement(12286..13170)
                     /locus_tag="PN977_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001642856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HDI6041870.1"
                     /translation="MPEESKGFLSVEEVAGNTGEIHSLKPNNNSTIQPIALLRLGVFV
                     PTLKSTNVALRRGSSVTTNTTNATEELSSLKIVEQEGYEGIEIHGPRLDMDTDFKVWV
                     GITSALFDYAPDDDGIITLPFSEFADRCGYPRKRLSKAFRKSIDDSLTRIQQTVVKFR
                     FPAAKGHLNNINVNLLAYSSLNTELDVIEIQPQKQLSELYYVDYKRILKLKMLDKLGR
                     KETAKVLYTFFEALPANPAPVSIERLRARLNLKSSVSVQNSVIRKAMKDLEAIEYLKF
                     SEIKNGRKIGFQIHKRNP"
     gene            complement(13505..13897)
                     /locus_tag="PN977_001835"
     CDS             complement(13505..13897)
                     /locus_tag="PN977_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001281114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6041871.1"
                     /translation="MSDKVTVKQTINKATSIYKIEHITVGKPGSEQYRHAFELAAQLG
                     LKHPDCIEHVFPTYADEQCTHVLTEEDFFSTEEREGVDRCIGVICSSVSYELFPNVHE
                     NGGIGYQFLYEGDELKCYEHGLLIESVE"
     gene            complement(13909..14049)
                     /locus_tag="PN977_001836"
     CDS             complement(13909..14049)
                     /locus_tag="PN977_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000336812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6041872.1"
                     /translation="MCRMPEFSSEYFDDLPAYILDTETMLMGINRKNRNVNDYNRAIS
                     GN"
     gene            complement(14075..14497)
                     /locus_tag="PN977_001837"
     CDS             complement(14075..14497)
                     /locus_tag="PN977_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001615620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ppfA"
                     /protein_id="HDI6041873.1"
                     /translation="MSFFSTLKTALSLKEKLAATGVLVLICALVGAGFAWERHQLKQA
                     MEKIGSLDQAVKERDKSIMDLNQTIETMNKAEQHFHSQEVKNESEQAKYADRQMERKA
                     EVQKQLVAAGNVRQRIPADTQRLLRESISEFNADADKG"
     gene            complement(14537..15325)
                     /locus_tag="PN977_001838"
     CDS             complement(14537..15325)
                     /locus_tag="PN977_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6041874.1"
                     /translation="MLEKDYQLSAYKKLAAAGGMKTPGAITSARNSANTAKLLAEELT
                     GLILDTIVYPDTITSYVSTIRTTATGLTNIGGLATQHADLLAGYADLSMLLQLDIGWD
                     VYCRANEREVSELPISIAIGDVTITKSLEDAVNALNTSSLVAAMGEINQTLNTGSGSS
                     SGSGSGGGTATPPPALTEEQIESLKVATEQFGVVFNQTTAPTTALQQQYERANESANV
                     AITAYNHAIGTALAEASANKASTASAIAALVPDSVLDELNKAAQ"
     gene            15788..16072
                     /locus_tag="PN977_001839"
     CDS             15788..16072
                     /locus_tag="PN977_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001177860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6041875.1"
                     /translation="MQMELISRKEFDSRVTSGELDNLQAIKVKEGFCLIGNQSGTNRV
                     FMLRRTDLKPFVWKNEIGPSSYAQTRGCHNLAFFYKDELSVVDIQGLQHV"
     gene            16065..16970
                     /gene="rdgC"
                     /locus_tag="PN977_001840"
     CDS             16065..16970
                     /gene="rdgC"
                     /locus_tag="PN977_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001702258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-associated protein RdgC"
                     /protein_id="HDI6041876.1"
                     /translation="MFKHWKNITIYKLSREADLTDLEDKKKMILFTPCGSQDMAKFGF
                     VSPFGDNSEVIAMHGNGFILVEAKRETKILPPPVIQRAIQEKIEKLEQEQARKLKKTE
                     KDSLKDEVLHSLLPRAFSKFSVIQAIYDGSTKRIYINASARQAEDMLALMRKSLGSLP
                     VVPLSVENPIELTLTDWVRDGSAPQGFQMGDAAELKAVLEDGGIARVKKQDLGSDEIS
                     THLEAGKLVTKLALDWQNRIKFTLDHNFSLTSVKFADELLEQNSDIDSEDVAQRLDAD
                     FFLLTSEISCLVDALVNALGGEAKQ"
     gene            16967..19390
                     /locus_tag="PN977_001841"
     CDS             16967..19390
                     /locus_tag="PN977_001841"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001702256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dam family site-specific
                     DNA-(adenine-N6)-methyltransferase"
                     /protein_id="HDI6041877.1"
                     /translation="MKELCYGSVCSGIEAASIAWEPLGMRPAWFAEIESFPSAVLAHR
                     WPHVANLGDMTKLAQKVQAGEIEAPDVLVGGTPCQAFSIAGLRGGLDDERGALTLKYV
                     ELANAIDDKRSESFLKPTVIVWENVPGVLSSADNAFGCFLAGLAGEDAPFEPGDRPES
                     RKSNTFWRWDVKTGCHAPKWPQCGCIYGPQRKVAWRILDAQYFGVAQRRRRVFVVASA
                     RTDLDPATVLFEFEGVRRDIAPSRGEGKEVAGNVGNGIKSGSHWDNPVNPHPTLNQSH
                     NTGGIGASNQEIFAQRGSGLVYCMAHGQGGAEIKNDGTSPTLTCNHEAPIVFSCKKAI
                     AFTSKNYGQDAGVEISPTLRAGNSANSNQNAGCPPAVAYSLQHAQIGRKDDAGPQGKG
                     WQKEISFTLDSRATADAVALSFWGQESIEKGELGENGYLPHMMSVRRLTPVECERLQG
                     FPDGHTLIPTGKRKKVTSDELIYLRNHDPDLSEEEAAMLAADGPRYKAIGNSMAIPVM
                     RWIGERITKAACRQKEGSETKERKVKPAAEFERSIFKWAGGKFGVLEQIFRYLPEGKR
                     LIEPFVGGGAVFMNAGYQENLLNDVNADLINFYKTLQREAHSLITLAHRFFQDYNTQE
                     GYLAVRNAFNKQVYDDLHRAAAFLFLNRHCFNGLTRYNQAGEFNVGYGKYKTPYFPLQ
                     EMEAFLGAEGRSEFVCGDFAAVIEAAGEGDVIFCDPPYEPLPNTEGFTNYSGHDFKFE
                     EQKRLVSLLTDAHRRGAKVLITNSGAPNIRELYQDSGFRVEPLFARRSVSCKGDTRGV
                     AHDVIAILL"
     gene            20066..20141
                     /locus_tag="PN977_001842"
     tRNA            20066..20141
                     /locus_tag="PN977_001842"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:20099..20101,aa:Asn,seq:gtt)
     gene            20144..20219
                     /locus_tag="PN977_001843"
     tRNA            20144..20219
                     /locus_tag="PN977_001843"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:20177..20179,aa:Thr,seq:tgt)
     gene            21147..21572
                     /locus_tag="PN977_001844"
     CDS             21147..21572
                     /locus_tag="PN977_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001481567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite/colicin resistance protein"
                     /protein_id="HDI6041878.1"
                     /translation="MLGILKKKFRKAAGGVKKMENRDAVEATVWGAYSIAYSDGTCDA
                     KEIAVLEKTIAALPAFAPFSGEIAQMSANIRAQYEASPRRANAQALRELADVSGTNDA
                     VDVLCLCIDIADQDGIGAEEQEQLKKIAQALQLPLEQYI"
     gene            21572..21736
                     /locus_tag="PN977_001845"
     CDS             21572..21736
                     /locus_tag="PN977_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000234829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3927 family protein"
                     /protein_id="HDI6041879.1"
                     /translation="MVIKARLILALVFLVLSVLVDFTSTILSVLSDGALVAVAVTLVW
                     PIFKPASKDQ"
     gene            21847..21922
                     /locus_tag="PN977_001846"
     tRNA            21847..21922
                     /locus_tag="PN977_001846"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21880..21882,aa:Ile,seq:cat)
     gene            22209..23573
                     /gene="dnaB"
                     /locus_tag="PN977_001847"
     CDS             22209..23573
                     /gene="dnaB"
                     /locus_tag="PN977_001847"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001276607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HDI6041880.1"
                     /translation="MSASPLESMPNSLSAEQAVLGGLMLDNCRWDEVADRIVADDFYT
                     SAHREIFSEMERLLSHGKPIDLITLAEALEQNGKLERAGGFAYLAEMSKNTPSAANIC
                     AYADIVRERAVVREMISVANEIAEAGYAQDGRGSNELLDMAERRVFEIAEKRQKSGSG
                     PKDIASILDATVSRIEELFQRPHDGVTGLDTGFTDLNKKTAGLQASDLIIVAARPSMG
                     KTTFAMNLVENAAVRNDKPVLVFSLEMPSHQLMMRSLASLARVDQSRIRTGQLNDEDW
                     ARVSGAMGILLDKQNIFIDDSSALTPTELRSRARRVYKENGGLSMIMIDYLQLMRVPE
                     LQDNRTLEIAEISRSLKALAKELQVPVVALSQLNRSLEQRADKRPVNSDLRESGAIEQ
                     DADLIMFLYRDEVYHPDSEMKGIAEVIIGKQRNGPIGTVRLAFNGQYSRFDNYAGADW
                     QEDY"
     gene            23573..24571
                     /locus_tag="PN977_001848"
     CDS             23573..24571
                     /locus_tag="PN977_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001198668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6041881.1"
                     /translation="MQWNEEKPMNILIIGRKFEAISDVKTYTEMWAYNLACAFSEAGV
                     TLQYHRPYSPGVESPEDYVEAVLTAATSCSAKAILAPGLRYFTTVPREIGVQLRRRFT
                     GWVAQVYDGSMLDSAPVDITFTVRDDTWRYLDNPGRLERHNRFNKHVGWAANQELFHL
                     ETKTDDVLRIFVDHAAFDVSGFDHSLSILMNLQRLTVPYEARTLTDDGLVTIDPGNIS
                     VTPYRRTPVPATEFAAELRKSDVFIVTHPESLGLTVLEAAMCGALVLTPPDCLPPDRL
                     ALVNHMVIKSRIDWDEVIARVDRVKNAEKVQCHTWSAIAEKMLETFITQKPSCGNG"
     gene            complement(24617..25249)
                     /locus_tag="PN977_001849"
     CDS             complement(24617..25249)
                     /locus_tag="PN977_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000535207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate protein"
                     /protein_id="HDI6041882.1"
                     /translation="MGSLTGKYRAVVVSVDDPKGLMRTQIRVVGMMDGLPDASLPWAE
                     AILSNANTFSPFLPGDKVWVEFPYNGDSRWPLIIGYAQDASGGAPNVPPEASGQGEGY
                     VPPEVEGAPAQPSTSAKKDFISSRNGLMEVRTAGGAWAVTHLKSGTTIGFNEAGELYA
                     ISQGPAFISSAGNLDIKSGADVALKAGGSMAIEASGNLSIKAAQVSVDKA"
     gene            complement(25242..26258)
                     /locus_tag="PN977_001850"
     CDS             complement(25242..26258)
                     /locus_tag="PN977_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000212027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HDI6041883.1"
                     /translation="MATKTTTAPETDSKRTQLFLQSVSIGQNEIPREMIVGCTYVEPG
                     ELSGPQLMLMVRDSTAYVVNKLGVKFGTILTVSLGDPEGHGGILFSEEFFVLKAPRKD
                     DTVLIYAFSNPVRLLKVPSTSAQYFVDKPPSAVVSSLAPGLKVNADSFRKTSTYHLNV
                     GEKPTKVLQEIARDTGSMCWASRGTINFKSMEKMANAAPSLTYESANPNTSGFTISQF
                     NILNADYEYQRRHNYRMASYDMTKGVVYSGNQEDPIKFTSNPDPTALANYNKFILPRL
                     DMLVEGNAALTPGTTLKIVVHNTAGDGELDESIPDKMIVMSVTHFEDRFRFVSRAQLG
                     VVNG"
     gene            complement(26260..27045)
                     /locus_tag="PN977_001851"
     CDS             complement(26260..27045)
                     /locus_tag="PN977_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate"
                     /protein_id="HDI6041884.1"
                     /translation="MILNNQEWLLAIFKKKGLTPTGKLEFATIDGIDSALAQALNEAF
                     DSQVVSFNDRANQSFREFLKRTPRDRITLGTFSDVKEWLSSFEADRAGRKDTVSSGPV
                     NKLAMPLVNLSRSPAFSIYEGELCRDNYEEGQVTNENDEIEALVSTIPFSLEYSLWIA
                     SDEKESLGMVSTALAFWLRMYASLGQASFTHIANVGGYEIPVTCYIEGQKSIAFQDLT
                     TGTADNRLFAVGLNLTVVAELPILAYMKQTNGTITVKAKILEA"
     gene            complement(27032..27760)
                     /locus_tag="PN977_001852"
     CDS             complement(27032..27760)
                     /locus_tag="PN977_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000896792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDI6041885.1"
                     /translation="MAGFFDDMFEDTEPSQQVTGDNLPDTESDPDIPGEGSELIEEED
                     IDAEIETDGVNVGNIVDPVEDNHLPNLDHGLLSDSGVRHRYQGHAVFNNLVRMDWLKA
                     IKLDPDSFDAVLYRAIPYRDKNAPETASEIIEPNQRIYDYQDPELITALDCPDEMDAF
                     YALYDGSDNTGISDSALILRLAAVNVPVGSMLEWLEQLSDGTTIRRFWYIHKIFNYGT
                     ARVGSLFYCVPSRAFEGNFIGDSE"
     gene            complement(27764..28981)
                     /locus_tag="PN977_001853"
     CDS             complement(27764..28981)
                     /locus_tag="PN977_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001141912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDI6041886.1"
                     /translation="MPTEYARDNLGRYQTDGLSAKDFNKVFDLIRKQQRQNRRNARRT
                     LTPRIMGMRNRELEAFLSLGKKKDGTYFTPEDIRSFNTSRQAHKTKFKSTVPGITYAQ
                     LVAQSTSIDIKRANNKVSDGTGIKAATFLGLKHNLALISVNASDESVHQHHRVRIRFE
                     EWDKAVEDIAEDGAKKARIAADLCKGRVSFDCDCGRHQYWYRYMATAGNYAVAPPKEY
                     AFPKIRNPDLTGVACKHVLHAMTRFQSPTWHKAIIIALEKAAEQVAFGDDKRKTTTYF
                     KGELAKSLARNRTTTTDQAKAAREYELYLKSQDALGKKLRAKDSATDNVRRLLKKART
                     TANRKNAELKASRVREAQARAEADALKKALQTQANNLIKFFMSQGMDKAAATAQARSI
                     LETQINEARKRKG"
     gene            complement(28991..29368)
                     /locus_tag="PN977_001854"
     CDS             complement(28991..29368)
                     /locus_tag="PN977_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000235789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6041887.1"
                     /translation="MATSITTTQSTRQYPLSRYDDRNIADPILRAELRKEVMLMCESN
                     DKNLTIYYVLPDEQYRPDLLAYRMWGIAELRWVVTLAAGLEDESQGMTVGKKLKLPPA
                     TWIREMIRHFQYDGQVIGTLSIA"
     gene            29515..29758
                     /locus_tag="PN977_001855"
                     /pseudo
     CDS             29515..29758
                     /locus_tag="PN977_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000840933.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            29761..30339
                     /locus_tag="PN977_001856"
     CDS             29761..30339
                     /locus_tag="PN977_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001615599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VRR-NUC domain-containing protein"
                     /protein_id="HDI6041888.1"
                     /translation="MLRFTEEEFQAFSERRNKGRSRPKTKKDPFLSLAPVKEVSPHAK
                     ALAALAKNPDLRDGNCEHFEQVFIFDYFERKHPDIYELLHATPNGGKRSKATAGKMKA
                     EGQKKGYPDMSLDKACGIYHGMRIELKELNGKAPTKEQIAWMRRLREEGYYVVLAYGA
                     EQAITAILEYISLKKGEAIEHVLNGDKWLHAS"
     gene            30406..30561
                     /locus_tag="PN977_001857"
     CDS             30406..30561
                     /locus_tag="PN977_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000095381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="norphogenetic protein"
                     /protein_id="HDI6041889.1"
                     /translation="MSSKVNYESLASVMPRNEQETDAVVDPVIAEMNARLEAEFAAEN
                     EHTTQGD"
     gene            31063..31689
                     /locus_tag="PN977_001858"
     CDS             31063..31689
                     /locus_tag="PN977_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001569378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="norphogenetic protein"
                     /protein_id="HDI6041890.1"
                     /translation="MFPKNKFRGSDRVIIVGSGPSAANFVAPRGVPIIAVNGAIDWLN
                     RASYFFTLDPSPDNMRRVGRGRRRRGVCYCMALPDVKEREVRDGVLCFRRVAERGTEP
                     KNTNSPEWWAWRWSAHFGLCEDENEIASGNSAYGALNLAFHIGFKHVALVGVDATQEP
                     RVHSGGTPKNLSHLPLLFQSAREQIDVVSCGKMGGIPQMTLKEWLKNT"
     gene            31686..32363
                     /locus_tag="PN977_001859"
     CDS             31686..32363
                     /locus_tag="PN977_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001372319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HDI6041891.1"
                     /translation="MMAPTIYHRIDGTKYRNVWVVGDLHGCYTRLMSELHRVDFDPAQ
                     DLLISVGDLIDRGTENVECLELLQMPWFRAVMGNHERLMLDALSPDGNVNNWLMNGGQ
                     WFFMLDTDQEILARALVELVRRLPYIIELNTGQETIVIAHADYPDNEYQFGKEVPLFN
                     VVWARERISDSMDDIGGEISGADRFIFGHTPVKSPKTFWNQHYIDTGAVFCGNLTLIK
                     VKGDGAA"
     gene            32360..33061
                     /locus_tag="PN977_001860"
     CDS             32360..33061
                     /locus_tag="PN977_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000684845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6041892.1"
                     /translation="MKIALVLRSGGDYNASDVQWLVNQLPKGYEIICLTDLKRLHVPG
                     VKVIPLINQWQKCRGWWAKIELFRPDITDDLFYLDLDTVIAGDIRPILENPPTSFTML
                     RDFYHPQYRGSGALWIPNSVKAHIWSAFWQDPEGWIARCVTTECWGDQGFLRKVMGDD
                     TPAFQDLYPGWFVSYKADVVEPGSKYASARYSRGNGTLPKDCRIIFFHGKPRPREVSE
                     DWLPLISSFFERESE"
     gene            33363..34625
                     /locus_tag="PN977_001861"
     CDS             33363..34625
                     /locus_tag="PN977_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001468757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6041893.1"
                     /translation="MSTSAQNQSIENVSIPDVLNAGIPAIIQNIRAAQRRVSCDDLTA
                     RFFDNAVQSAEMLHAQLIDVYNAEADSHNSLVDAAENMQLDLGLKGKEIEELQLEIEH
                     LKRQQQDAIDDATHDANQRADNAERISIELETKLNEMTAMVELRNSQISTLKSQYKEI
                     MKLDPFNLEKRYNKAKSERQELRKQVADLNQQLKKTIKDASEARVAFANKKAEVTALV
                     NENAKFATLKKEMYGITERRFPASKLHPTLGQISFFPRLLAYGISSPKEFNNERPYIV
                     SKLDFAYQFCCDMGYAIDIRINEWLMPNFQPLAIFREFQPEGWVEFFHEMICKEMESR
                     RPELVRRVEWAQEVMLADAELPFEPEFIDELANKGLHSLFDVVTRRHEQLVVELGLEE
                     TAARRLLDVCYARSDAWEKENGGTIYVR"
     gene            34698..35204
                     /locus_tag="PN977_001862"
     CDS             34698..35204
                     /locus_tag="PN977_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001468756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-phosphatase"
                     /protein_id="HDI6041894.1"
                     /translation="MSIFNKHAHQERPYIVIVDIDGTISEATEDRLHLLPPPGKGALT
                     EHWNEFNLACDTDAPITPVIDMVRQLSGIYTLWFVTGRCEIARDKTRAWLRKHVTNGA
                     EPLLSMRPATDDRNDGPAKIDLLKKIGLSKIAFALEDKIEVARVFRSHGVLTLMVREY
                     ENALLHQQ"
     gene            35399..36124
                     /locus_tag="PN977_001863"
     CDS             35399..36124
                     /locus_tag="PN977_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000675631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6041895.1"
                     /translation="MKGEVKERGMIFNDEMVRAILEGRKTQTRRIMKNQPAEVGPEAP
                     VMVREIGAGFQWYGADGVSSVFNCPFGIVGDRIWVRETWAILGNEDGCSVDWNDNLCR
                     GDEKNAARIYRASCEQKPGDYGLWSIPDDADWKPHTVNEKFDGGWRPSIHMPRWASRI
                     LLEITNVRVERLNNISECDARDEGVPPAGSLLPDHPGTFLTPKGDFAMAKVAFQRLWE
                     SIYGEESWNANPWVWVIEFERIQ"
     gene            36164..>36329
                     /locus_tag="PN977_001864"
     CDS             36164..>36329
                     /locus_tag="PN977_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1382 family protein"
                     /protein_id="HDI6041896.1"
                     /translation="MNKASPAELRASLEMAHSLAQIGVRFVPIPVETDEEFHRLAASA
                     AQKLEIMAAKV"
BASE COUNT         9414 a         8465 c         8983 g         9467 t
ORIGIN      
        1 gcgccaacag tacatcaacg ccgggtattt cacggtgaaa gaagtggtgc tggatgatga
       61 aaatggctac cagatacggc tgacgcccca attaacgggt aaaggccagc agtggttaac
      121 tcgcaagcta cttgatgctg gtttgttaaa accagtagca ataggttaac aaaagaaaaa
      181 aacctgccag caaactggca ggtttctgag cagatcgtcc aacccgatct ggatcgagtc
      241 agaaaaattt gctctaataa atttcgtttt ctaagtgcaa agaatcacca tttcgagctg
      301 gtgattgaag gttgatgcaa atttggagaa aaaatgcaac aaacattcaa tgcggatatg
      361 aatatatcaa accttcatca aaatgtcgat ccttcaacca ctctgcccgt tatttgtggt
      421 gttgaaatta cgaccgaccg cgctggccgt tacaacctta atgctctaca cagagcgagc
      481 ggactcggtg cccataaagc gccagctcaa tggctaagaa cgctgtcagc taaacagctc
      541 atcgaagagc ttgaaaaaga aactatgcag aattgcatag tttcgttcga aggccgtggc
      601 ggcggcactt ttgcccatga attgctcgca gtggagtacg ctggttggat ttctcccgcg
      661 tttcggctga aggtaaacca gacatttatc gactatcgag ccggaagatt acaacctgct
      721 attccgcaga gtctccctga agctctccgt ttggctgccg acctggcaga gcaaaagcaa
      781 cggctggaac aaaaaatgct tatggatgca cctaaagtcg aattcgccga acgcgttgct
      841 accgccagcg gggttctaat cggcaactat gccaaagtgc tcggtctggg ccaaaactat
      901 ctcttcacct ggttgcgtga taacggaatt ctgatcgcaa ccggtgaacg caggaacgtc
      961 cccaaacaag aatacatatc ccgtgggtat ttcaccctta aagaaaccgt gatcgataca
     1021 agcaatggaa gcaggatttc tttcacgact cgtataaccg gcaaaggtca gcagtggctg
     1081 atgaagcgat tgcttgatgc tggtgtgctg gtacctgtcg cggcaacgcg ctaacagacg
     1141 tagtaagaac caccagcatt gtaatgctgg ctaaagtcac tttcctgagc tgtataacga
     1201 tgagcgattt tactttttct ggctatgaat tggcctgctt tgtaacacac tccggtctat
     1261 cccgtagcgc cgggcatatc ctgtcgcaat gtgcaaatct agcggcaaca accagtgaat
     1321 acttcattca caagcctcac cgcctgatcg cggcagaaac tggttatagc caatcaaccg
     1381 tcgttcgtgc attccgtgaa gctgtaaaca aaggaattct gtctgtagag attgttatcg
     1441 gcgatcaccg tgaacgtcgc gctaacctgt accggtttac accatccttt ttggccttcg
     1501 cacaacaagc caaaaatgcg ctgattgaaa gcaaattaaa gatctcttca gcggcaacca
     1561 aggttaaagc tgttctcgct aagacattgg ctttatttaa ttttttatcc acacccccat
     1621 gtcaaaatga taccccctcc ccctgtcagg atgacgtggc aataaagaat aagaagtcac
     1681 aagttaaaaa aacaaaaaga tcagtttccg gcggtgccgg aacgaccaga ctcaaaaaat
     1741 tgacttcatg gatcgctgag gcaaaagcaa aggctgacaa tctgcggtta tccaaaaaac
     1801 gcgctcaaaa acatgagttc aagcagaaag tagaggcggc agcgcggaaa tatgcttacc
     1861 tgaaggacaa gcgttctcct gatattggcg ggatatcaaa cttcgataat ctgccgcatt
     1921 gcatgacggt aaacgaagct cttaatgcgg ttttagccaa aaataaagat aacgaacaat
     1981 ggggtatacc ggcaggattc agagggtgat agattgctct aatctggagt cacctggcgt
     2041 tttcagtttg aggtcggaga tgcaatctga ttttttacag ttagcgatcg cttttgcagg
     2101 atatgtttgt attggcttct gtgtatacat gatcagccga aaaatgcttg tcgatatcga
     2161 ccgcaaagaa caagcagagg agatcttaga atggattttc tttggcgcgg tctggccatt
     2221 agggatcatg tttgctgcaa catttcttct gatgtggata ttcacccttc caggtgattt
     2281 ctatagaaaa aaagccagac attgatacaa tcgttgcggg tgcttgaggc tatctgcttc
     2341 aggcattacc cgaaaagcag atagaagaaa gccccagata acattacgcg tcctgcaaga
     2401 cgcttaacat taatctgagg ccatatctat gcttagcata cgtagattaa ccctctcccc
     2461 ttttaaggag caaggaattt tttactgcta tgccgcattt ccaattatgg attcagcgac
     2521 cagaacaacc ccatgcttac ttcgatctgg gtgttcactc catatctcgg attcaggaat
     2581 acttaccccg tcatagcaaa cacgttcgcc gtcaaacggg taatactgag cggcatcggt
     2641 gtcaaggata aacactttta gctcggaaga gcgacgggcg atgtgttgcc aaagagcttt
     2701 tccaccttcg tattgctcat tatcactgag caaaacaaca ccagcttttg aggctacggt
     2761 ttcgtaaaca aaagtagcta aaccgacatc ccggacggtt ccatcaacca cgataccttc
     2821 aatctgggca acatcgctat cctcccagaa cttccttacc agtacgcctt caagctgcgg
     2881 gcgacgttga agttcaagag tagcgacaac cttatggcgc atatcaccat cgataataga
     2941 aggcgcagcc tctattacag ctgcaaatgg ggttttggaa tcccatataa cctggtaaag
     3001 cacaccttgg atggtaactc cgtccaggat cgaaaatcga cgagcgacag tacccgggga
     3061 gtacgacatt ggaatcacag ccattttaga gtcccccttt aacagtacag gcacaaacag
     3121 ctacctcaat aattgtagtt tattagaaca ttaataatct aacataaaac tccagacaat
     3181 gttatcgatg catgtttata taaaatagtt ataaaaaaag ctcccgaagg agctttaaaa
     3241 tacaagggat gactcttaat cccactcaat ccagttgtag acgatacgaa gtgacgggcg
     3301 cacagcggca gtcacatctt cggtactaaa gtcgattgca tcactgtaga ttttgcagtc
     3361 caacatttca attgttgtag cagcttttgt cacagcgtta accccggaag atttggattc
     3421 aggggtcgca gccatcgtga tatcaacata gtccttcgcc gcaatgcgat ctttaatgaa
     3481 ctgaagaata tcgccttcga tagtctccac gcactggacc tggatttccc cggagttacg
     3541 gattggaccg tgctggttga acttcacacc atttgggcca tagtcctcca catcctcgcg
     3601 ggtcatttca gggatttgcg acgtgcgaac cagtacgctg atatcttcat agcctgcaaa
     3661 agtgagctgg aattcagaag ataccagtcg ttcgcctttg gccgcgttgg cagtatagcg
     3721 gcccttaata aatttacggt ttcccttagt gttattgtgc cccatataaa atccttttac
     3781 tggaacgccc gaacaatatc ggagctgtta tatatcgaag aaccggtcaa ctggaggttg
     3841 acggtgtttt tcaggaaata tccattactg tcccttggcg catcgagatc aaaacttaag
     3901 tcctggatgg cgacatcggt gatgttgatc cggcgaccaa tgtttagcgt cacgcgctcc
     3961 gggattcgac caccaacatt ggccgctttt agttccgggc taatcatggc tgtcaacgcg
     4021 gcgatagcgc ctgaaacttc aatgaatgga ttgttcaaag caatgaaagt tacgggcagc
     4081 gtgaacgtcg gcggagtccc cccttcccaa accattaagc tattccaacg ggcaacagac
     4141 gttgtttcag tacctacttg cacaaaacca cccagagcac cagaaacaga tcccatggac
     4201 ataccggtaa atggcgcttc ccaattctgg gccatgttca ttgccgctcc ctggctgata
     4261 tatccggtaa cctggtactg agagttcgtt aaagtaactt tcagatatgg cgatacaccg
     4321 tcagcctggc tgtaaacccc ataagctata ggagccattc aagttaaagg ccggagttct
     4381 ccggccccct cctttagcca aggcgcttac ggcgcagttt cattgacttt ttgcgggcaa
     4441 gttttgccgc gccggtctgg gcttttcgac gcgctttttt cagcgccgat ttttgagccg
     4501 cagtcagacg ttttttacgc aggcgtttac ggatgagttt gatctcaccg ttacgaacaa
     4561 ccttcttaaa tgcttcagtc agcatttcat cagaagtgcc agcaacaaca aacgccgctt
     4621 ccagttcgtc acggtcgtcg ctatctaaac cagcgataga ggcaccaaca tcagcagcag
     4681 cgtcgtcgtc ttcatcgtca gccagtgctt cgatcaggtc atcatctaca ccgcatgctg
     4741 cgaggaagtc agcaacattt gcccatgctt cgttataggc atcgtcctgt tcttctgtaa
     4801 cttcggagtc gtcgtcatca gagataccag cgatagcctg aacgaaacca tcaagggagt
     4861 cgaaagtcag atcaccgcta tcagcccagg agaaaacggc gtcggccgca tcactcaacg
     4921 cattctgcat agcacttcga tttgcagctt ccagaatcat ctggtgcgcc tgttcgacgg
     4981 tccattcttt accgtctttc ccttccagga tttgctctgg agccggggca gatggaacgt
     5041 tatcgttagt ctgtgccgcc ggttccggat tattattaat aaccggatct gttggcggtt
     5101 cggcgcttgc tcgggcagac tccatcagct gcacaggatc agagttcaaa gcgaaacggg
     5161 acagtccatt ccccaaaaat gccccggatt gaaaaaagtt tttgctcatt gtattccctt
     5221 acttaataag cagcggtacg ccctggatac gacgggctac gccagtcggg cagcaggccc
     5281 agactacttc ccatttatcg aattccgcct gcgtaacttt cagcacatac ggttctgtac
     5341 cgtcagcatc aggatcacga ggagccacca gagcgccgga ggcgacaaag cgatctaaaa
     5401 gtttggtcat ccctttagtc aggccagccg cagtaatacc gtccgggcta tgcttcatct
     5461 gtcgggctaa ctggacaaag aaacggctga ttgcattcat cagggatggg acgtgctgga
     5521 agtgcagata gttatcctgc gtgcagcaag ttaaagcatc gtcgatgatc atctggccag
     5581 aggtgccaac agatacttta ttgagacggc ccttgaccat tgcttcttcg tccggggtat
     5641 cttccggata cagcggttga attgacgcac gagcaatgac ggcacgttct tcaccagccg
     5701 gtgagtaatg ccaaccgccg acatcagagt ttttcttgac gccacgagct ttcgccgcat
     5761 acgccgcgcc agacagaccg aagaccacac gggattgggt ccatttgtct ttgcaggaga
     5821 acgggaagtg atagacagca cagcttacat aatcggtacc aagtaaaccg gtatcttcaa
     5881 cagcagagag cgcttccgta tacgtcaatg tcggtttgac atcaaagaag ccatcaatca
     5941 ggcgatcaga acagatatta cctaacgcgg tgatcgccgc attgtcatag caacccaggc
     6001 cgagaacagc ggtgtacatg tacggcgcat tattcagcac tttaaccgca cgcaggtacg
     6061 cagcggttga gattttcgac tgatcgccgt tggtaccgcc agtgaacgcc aacgattttt
     6121 tgtttgttac tttcgctgtc gaaatcagct cttcattaac aaccgcgcgc agatatttag
     6181 aacgggcttc cagagccgta ggcagataac acaagcggcc catgtcatct ttcgcttctt
     6241 ccgccaaaga cacagtgtgt gtctccaggg tcgttaccac gccgagcgaa gtcgtctggg
     6301 tcagttttaa gaggaagcgt tcattacccg cgctgtccgc tgttgccgtt tcgatggtta
     6361 actcacgggt aggtgaaata cacggatcac catcatcaac gtagatagca aaggcttcgc
     6421 cgctatcaag ttcaatttca gaaccgtatg gcaacgcact gtaagccggt tcgcctgatt
     6481 catcgaacat aataatcggg aacttcgcat catccggaac agcgcgaaca acataaccag
     6541 acgtttgctg aatagcttcg tatacatggc gaattggttc gaactgtgag ccggaagacg
     6601 gcttcagcgg ttcgccgaga acatcttcgt aattggactc agtaaccgca agaacagtaa
     6661 acggcttgcc acgcgcaaat acgccaatac cagcccataa gctgctattt aatgcaacac
     6721 cggtagataa cgtcgcatcg gcattgatcg ggctaaccgc gacgccggat gcattaccta
     6781 atgactgttg aattgaatat tgagacataa ctttccctgt tatgcgcccc gcacgggggc
     6841 gctatgttaa acggagaact tcccctgatt actcagagtc accggcatca atcgtgtcgc
     6901 cgctaatgaa gttaagcccg ccttttttgg ccattgtcag cgttacacga gtgaagtaat
     6961 cagcgccgtt gcgtgggtgc atatcgttga tagccgaacc ccacagcgtg gtacggttga
     7021 ccagcgccgg agtggtcgga tgctggaacg ggatggccgg gacagcatca ccagtcacga
     7081 agcctgcttt acccggattt tcatcacgga cgtagcacag cacatccatc gagctgaact
     7141 gaatgttctc tgtcgttaag ttcttacaaa taccagcagg tacttcgtac actttcacgt
     7201 taccgaacag ggtaccgatg tagtgaacat acggagtctg gatatagtct tcggctggct
     7261 ggaagaaatc cttcggcaac tgtttgaaga aagatgctgc atcagcacca gcaaacatcc
     7321 ccatcgcacc agaagattta acgcgctcaa taatgtcgcg atatacagtc tggaatttgc
     7381 cacgaatgat ggttgcccat acatcaaagg actggttaac cggcagagcg atgtcaaagg
     7441 tgtcgttcgc aagagtacgc cagatcatga tgcgaagacg cagcatatcc tgttcatgag
     7501 acaggtattc cttcagggtg cggaactgta gggaacccag gtccagacca aattcacgct
     7561 gtgcttcata cgccgcctgt accgtgtgct cagccgcgat aacgaactgg cttgggaaca
     7621 gggtgtattt cttcatttcg tggttgatca gcgggatcag ctcaggagcg gcttcaatat
     7681 tgatttccgt ctcaattgcg atctcagtgc ctttatccgg cgctttggag aacgacaggg
     7741 caatctgacc aatgttgtag ttcagagagc aggtaacagt gatttgctca ccagcagcat
     7801 tagtaaacga gtgaagtagg ctgccggaac cgttatcaac aacagactta atacggttaa
     7861 cgtagatatt agtgcgacct tttcggattg gtacattctg gccttcgaag tcttccatct
     7921 tgaaggttgc ggttttgctg gtgccatcgg agcttgccac cagcacatag cggcggcgta
     7981 actggctgta cacaccgacg gattgcatgt ccagaacatc accagcagca taagaaccaa
     8041 aagaggaacc tgccacgtta aagacttcat agatgtcgga ctggtcacgc gtaaccggaa
     8101 tgaaggtaca cgcatcagcg gtagctgccc ccaactgaac aggcaggatc atcgcgagga
     8161 ataaaggcag acgcataaca ccgtcagaaa cgctcatcat ctctgctgcg acggattcca
     8221 gcatcgcttt attagtggca tccatgctat tgcgggtgga ctcaatcagg cagttttcca
     8281 gcgtctggtg gcaggaggcc agaatttccg gacgcggcat agatttatgt gctgcggcgt
     8341 agtcagccag tgcacttgcc cacgctgtag cgatttgagc ggtggcatta tcagagatac
     8401 ccgcaaaaac cgggtcttta cgtgcagctt caaggataga tgcggcacgc gcggcatcat
     8461 ctttaatgaa ttggttatca gtaccgaact gcgcagtgct tgcccagcca agcacagctt
     8521 tagagcgttt tgcgatatct gcaatacgat tctggtattc gcgtaagtta ctcaatttac
     8581 tcttccttaa acacaaggca cttgtgtgaa tcccttttcg gaagagattt tattgaaagt
     8641 cacttgttga ctttctcgtg acaagcaatt tttttatttt tttcgggagt aggggaggaa
     8701 ggtaaaatcc aaggtgaaat cgtggcgatt tcaccttgaa attttagatt gatttacttt
     8761 aaaaacagta ggttaacagt gaaatttgaa tggcgaaagt ttaaggcttc ggctttttat
     8821 cgaggctctt tctaaggata tgcccaatca tcctgtcgag ttcttcctgt agctcttttg
     8881 aaagtcgatt aaactcataa gaaaatgcac ggcctttcac gcgcttcctt gcaaagcgat
     8941 ccttgtcctc aaatttccat aattcagtaa ctacggactt atctttagaa cctttatccg
     9001 tgagtagtga ggcttccttt gttatcaagc gcaggatttt atttttaact tcatcttcgg
     9061 ccatttcttc aatggataag atgtcgttta tttccgggga tatgttttga ataagctgat
     9121 caaactctaa attcttgttc cccatttcgt cgccaacagc acaaagcgtt ttgtagtccg
     9181 aaaaggttaa ttccgactgc acagggaaaa gggcgactaa ttcttccgga gcactcgctg
     9241 cctggagagc acgcgtgacc ttcgcctgag acagtccttc tttggctgca atatccttct
     9301 gactcatccc atcatttttc attcgcatca aacgcagacc tatttctcga atgctgtgct
     9361 gcaatgctgt ctgaacgtct ttcgctaaat tttgcgcttc ctgaacgctg atctcctggt
     9421 ccgtgactaa aacccgcaac cctacgttct ctaagatggc agaagctcga cgccgggaac
     9481 catccaaaat ttcaattttc cctgtagccc gtctgacacc tattgcaggg taaaattgct
     9541 gatgcttaat agtgcttcgg atacttttta atgattttgg cgtaagagat gcctggtcac
     9601 gcccgttgtt atgctgatca acaaaggtat cgctttctac ctggttcgga ggtattacct
     9661 cttcaataaa tgtggcctgg cgaccagttg ataacttgaa tacctgctcg actcgatcgc
     9721 cagaggctga agaactatca aatccgctta atattgaagg attaagggtt cgcccaattg
     9781 ttggtctgtt tttctttgac atgggggttt cttactcctc agttagatct gataaattca
     9841 atacggtcaa aaactgcttt agcaaaatct tccgcagcaa ttcgcgcgtt cttcaatgca
     9901 tcagcactac caacatacgt tgccgggtta gctgaaataa cagtgtcaaa agactcgccg
     9961 cagcgttcaa aaccgtcaag gcgagggagg acaacatcaa gcatatcccc accgaacact
    10021 tctttagcca ggctatggca atacttatgg tctgccttgt tactcaactt ggacataaat
    10081 ccaatgttag ttgcaagctg acactcacag ccttcatccg aaatgagttt caccaactca
    10141 ggaaggcggg caacgtattt aagcgatgag tggaaatcaa ccgttgcagg cggcagaggt
    10201 gtaaacagta tattggccga ggccaaagca tttttcagga aggcgtcaag gtgaggacca
    10261 ctatcaacga ggataaagtc ataatcgctc ttcagcttat caatcacatt ttctttcagg
    10321 actgcatgga tgttctgacc cggtagatgc tcattgcaca gctctctcca atcggatgca
    10381 ataaaggcat cgtcaatcga cgcaggcata acgtcaaccc caggtacaac agaaggaaca
    10441 ataaactcct ctaacagctc ttcacggctt acattctgca acatagcctg tgcagatgtt
    10501 gcgtttacga taccaataga gtgtttatgg cttaaaaaca tcgttgctga agattgcgga
    10561 tcaaggtcaa taaccagaat ccttaaatct tccatcagaa gatgaggatg agcacgcatt
    10621 gcatgcgcca gagaaaccgt cgatacagtt tttgacacac cgcctttaag attggagatg
    10681 aaaatcacat acgcttcgct gtagcgatcc cggtattttg gcactccgcg atgttcatat
    10741 atgtcaatga tgttctgaat tgacatcgca tatttcattg aagagccagc agggcgttta
    10801 tcgaaaacat aacccttttc ttccatttca cttacggcat agtcaacgtt cgcacgagtc
    10861 agtagaggca attttgccag tgccgctttc gcatagacct ggtaaaactc gttcgcgtgt
    10921 agctcatcct tttgcaactg tacttgttca gtcagaacat tgagcattct atttgctctt
    10981 tgagcaacct tgtgaagctg gctggaatca ctcatcgaaa gtcatccttt atgctgtatt
    11041 tttgaattta attaaaaatg ctgcataaaa taataatgta tgcgtagatg cttgtacata
    11101 gcattctctg catgtttggt tcattttgca cgattgagag ttacaaggag ggcacaaaaa
    11161 agccccgttc aggggcatca gtgttatttg ctaagagcag cgaataatcg ttcgaaatcg
    11221 atagtatcta tagcacgcgt aagcgccgga agttcagcct caaagtaccc gtgtcgatcg
    11281 taaaagaagg gaccgaagag cgaggcatgt tggattctac ttcgccccag cccggacaca
    11341 cagttaagcc cattaccggc taaaaggcta aaaaacttct ctggattatc gtggtaaagc
    11401 tgggaatcaa tggtggcggt taactcttcc atagggaagc acacccgccc tgtatcccag
    11461 ggatatttag tccgaagcat aaacattgct ttcagcaatt cacattgagc gcggatcgcg
    11521 tccggttcat agccagatat ggagacataa gccacgtccc tcattcctgc gtcatctttg
    11581 aaagtcacga tagaagtaac atccagctct ttttcgaaag agcaagcagc atctactgga
    11641 cgctgaagta aatcattcga cttaatgcgc tcgagaatcc ctccccacat atcatttaga
    11701 tattcgatat gagccaaaac cttatcaaga cactctcgtg taaaccattc agtatgcccg
    11761 ccaccgacgc ttttctccca cggcgcattc caggggaaaa aggttgcgtg taaagcccgc
    11821 tcaagattaa ccattgccaa acgcgtacca cgatagaccc gtgaaagcgc aaaatcggga
    11881 ctcacttgta gtcctttaaa ccgtgccaat ggaccacatg aaatgccgat tttaaaagta
    11941 tctccgttct ctggcaccag aacgtagagg tagtgttgtt tctcttcttg catatcaata
    12001 ccactgcttg atgagaaccg cgcaaatgtt gactatgcgc gaaggttaat gtgaatagtt
    12061 gactatgcgc gatgtgacta cagtcaaaag ttgactgtag tcgatttaac tccaccaaag
    12121 atcgactatg taagatattg tcgggagaaa cgttgactat acgcgatgaa atgcccctaa
    12181 aagccatctc aatagcgact tgcagaatat tgacgccagc aaaaatccac cagcgtcaac
    12241 gaatgtcgcc tatagtcaac ttctcgctat cgcatatagt caatattatg gattgcgctt
    12301 atggatctgg aagccgattt tcctgccgtt ttttatctct gaaaatttaa gatattcaat
    12361 agcttccaaa tctttcatgg cttttctgat aacgctattt tgcacgctaa cggatgattt
    12421 gagattaagc ctcgctctaa ggcgctcaat gctgacaggt gccgggttgg cgggtagagc
    12481 ctcaaagaat gtatacagta ccttggccgt ctctttgcgc cctagtttat ccagcatctt
    12541 cagcttcagg attcgcttat agtcaacata gtaaagttca gatagctgtt tctgcggctg
    12601 gatctcgata acatcaagct cggtattcag gctgctatat gccaacaagt tgacgttaat
    12661 gttattgaga tgaccttttg ccgccgggaa gcggaatttg acaactgtct gctgaatgcg
    12721 tgtcagagag tcatcaatac ttttacggaa cgcctttgaa aggcgcttac gtggatagcc
    12781 gcatcgatcg gcaaactcgg agaatggcag ggtgattatg ccgtcatcat caggtgcgta
    12841 gtcaaacaac gcggaagtta tgcccaccca caccttaaaa tcagtatcca tatccaggcg
    12901 tggaccatga atttcaattc cctcatagcc ttcctgctca acaattttga ggcttgatag
    12961 ttcttcagtt gcgttcgttg tgtttgttgt aactgacgat ccgcgacgta gcgccacatt
    13021 ggtagatttt aaggttggca caaacacacc taagcgcaac aaagcgatgg gttgtatagt
    13081 gctgttgtta ttgggcttca ggctgtggat ttctcctgta tttcctgcaa cttcttcaac
    13141 gctaaggaag cctttacttt cttccggcat cacggtttct ccatgtgtgg cgcggcctga
    13201 cgtcaatttg gatggctgtt atcaacagct gtgaatattc agactctaaa atcgcttaca
    13261 gtcaatgttt ctgtcgcgta tagtcaacaa taaatcgcgt gcagtcaaca ataaatcgtg
    13321 cacagtcaac ataaaatcgc gtatagtcaa tgttgatccc atttcaggcc agtaatgacg
    13381 cggcttacag cgatccggga tcttctttgg atcttcctag gttctctttg ggatctgttt
    13441 attggatcta tgctgtggat aagttgaata aaccggccaa catagccggt tggaaggaag
    13501 ggtattattc tacgctttcg ataagaagac catgttcata acatttaagc tcatcgcctt
    13561 cgtacaggaa ttggtatcca ataccaccat tttcatggac attagggaat aactcataac
    13621 tcactgaaga gcaaatcaca ccaatgcagc gatcaacgcc ttctcgttct tcagtgctga
    13681 aaaaatcctc ttcggtaaga acatgagtac attgctcatc agcataggtc ggaaatacat
    13741 gctcaatgca atccgggtgt tttaaaccaa gctgagcggc aagctcgaaa gcatgacggt
    13801 attgttcaga tcctggcttg ccaacagtga tgtgctcaat tttgtagatt gaagtcgctt
    13861 tgttgatagt ttgctttact gttactttat cagacataaa aatccctttt agttaccgct
    13921 gatagcgcgg ttgtaatcat taacgttgcg attcttcctg ttaatcccca tcagcatcgt
    13981 ttctgtatcg aggatatacg ctggcagatc atcaaaatat tcactgctaa actctggcat
    14041 cctgcacata aatgcacttt ttgggtcagg gtggttaacc tttgtcggcg tcggcgttaa
    14101 attcgctgat cgactcccgg agcaaccgct gagtgtcagc aggaatacgc tggcgaacat
    14161 tacccgccgc aaccagttgt ttctgaactt cagcttttcg ttccatttgc ctgtcagcat
    14221 atttggcttg ttctgattca tttttcactt cctggctgtg aaaatgttgc tctgctttgt
    14281 tcatcgtctc aatggtctgg ttaagatcca ttattgactt atcacgttcc ttaacagcct
    14341 gatcaagact gccaattttc tccatggctt gctttagctg atgacgttcc cacgcaaacc
    14401 cagcaccaac aagtgcgcaa atcagaacaa gaacaccagt agcagcaagt ttctccttca
    14461 aagacaaagc tgtttttaat gtagaaaaga atgacatgtc ttcctcctga agaaaaatta
    14521 tcaatgaagt cctttgttac tgtgccgctt tgtttaattc atcaagaaca gaatcaggaa
    14581 ccaaagcggc gattgcgctg gctgtgctgg ccttatttgc tgatgcttcc gcaagcgcgg
    14641 taccgatagc atggttataa gcagttatgg ctacgttggc gctttcattc gctcgttcat
    14701 actgctgttg taacgcagtt gtgggtgctg ttgtctggtt gaaaacaacc ccaaactgtt
    14761 cagttgctac tttcagagat tcaatttgct cttctgttag tgctggtggg ggagtggcag
    14821 tgccgccgcc tgaaccagag cctgacgagc ttcctgagcc agtgttaagg gtctggttaa
    14881 tctcccccat agcagcgact aaacttgatg tattaagcgc gtttacagcg tcctcaagcg
    14941 atttagtaat agtcacatca ccaatggcaa tagagatcgg cagttctgaa acttctcgct
    15001 cattagcacg acagtaaaca tcccaaccaa tatcgagttg aaggagcatt gacagatcag
    15061 cataaccagc caacaggtcc gcgtgctgag ttgccagccc tccaatattc gttaaaccgg
    15121 ttgcggttgt tctgatcgtt gaaacatagc tggtaatagt gtcgggatag acaattgtat
    15181 ccagaattaa tccggtcaat tcttctgcaa gcagttttgc tgtgttagca ctgtttcgtg
    15241 ccgatgttat ggcaccaggt gttttcatcc caccggcggc ggccaatttt ttatatgcgg
    15301 ataactggta gtctttttcc agcatgatat ctcctaactt acctgaacca ggccgtctcc
    15361 ggctgcaacg gtagagccgc atgaaacagg atcaccaacg catacgatcc ctttcccgtt
    15421 gacggtaaac catgccctgg ttgatatagc ttgcccgccg tgcgtactgt ttccatcggt
    15481 atgctgtgca tattgcttac catcaactaa cacttctact ccgttgactt taagtagtgg
    15541 ttcactctct acaggaggcc tggatgggaa tcctccgtgc cccgaacaaa tgctgtcttt
    15601 tgttgcaata cttgccacgt tatcaccaat gatttgctct gattttcgtt attttaactt
    15661 aggttgtttg tggtctacat ggcgtttact tattataaaa ttgctctaat aaatattgtt
    15721 ttttatgtcg tgttttcggt accattcagc catcgccctt caatgggcat ttgtttggag
    15781 tcgtcagatg cagatggagc taataagccg caaggagttc gatagccgtg taaccagcgg
    15841 tgaactcgac aacttgcagg ctatcaaggt gaaagaaggc ttttgcctca ttgggaatca
    15901 gagcggaaca aatcgcgttt ttatgcttcg ccgtacggat ttgaagccat ttgtctggaa
    15961 gaacgaaatt ggtcccagct catacgctca aacgaggggg tgccacaacc tggccttttt
    16021 ctacaaagac gagctttctg tggttgatat tcaagggtta caacatgttt aagcactgga
    16081 aaaacattac tatttataaa ctttctcgtg aggcggatct gaccgactta gaagataaaa
    16141 agaaaatgat ccttttcacg ccatgcggta gtcaggatat ggccaagttc ggttttgtat
    16201 cgccatttgg tgataattcc gaagttatcg ctatgcatgg aaatggtttt atccttgttg
    16261 aagcaaagcg cgaaacaaaa attcttcccc cgccggttat ccagcgagct attcaagaaa
    16321 aaattgaaaa acttgagcaa gaacaagcgc gtaaactgaa gaaaacagag aaggactccc
    16381 tgaaagacga agttctgcat tctcttctgc cacgggcttt ttcaaagttt tctgttatcc
    16441 aggcgatcta cgacggttca actaaacgta tctatatcaa tgccagcgcg cggcaggctg
    16501 aggatatgct cgcgcttatg cgtaagtctc tgggttctct tcctgttgtt cccctgagtg
    16561 ttgaaaatcc cattgaatta acgctgaccg actgggtacg tgatggtagt gctccacagg
    16621 gatttcaaat gggggatgcg gcagaactta aggcagtgct tgaggatggc ggtattgccc
    16681 gagtgaaaaa gcaggatttg ggaagcgatg aaatttccac acacctggaa gctggcaagc
    16741 tcgtcactaa gttggcactc gactggcaga accgcattaa atttacactg gaccataact
    16801 tcagccttac cagcgtcaaa tttgcggatg aattgcttga gcagaactct gatattgata
    16861 gtgaagatgt tgcgcagcga ctggacgcag atttcttcct gttgaccagt gaaatttcgt
    16921 gcctggttga tgctctggta aatgcccttg gtggagaggc taagcagtga aagagctgtg
    16981 ctatggatct gtttgcagtg gaattgaagc cgcgagtatt gcctgggaac cgttgggtat
    17041 gcgtccggcg tggtttgctg aaatcgagtc ttttccatct gccgttcttg cgcaccgctg
    17101 gccccatgtc gcgaaccttg gcgacatgac aaaactcgcc caaaaagtcc aggctggaga
    17161 aattgaagcc cctgatgtgc tcgtcggggg tacgccttgt caggcattca gtatcgcggg
    17221 cttacgtggt gggcttgatg atgaacgcgg cgcgctaact ttgaagtatg tggagcttgc
    17281 aaatgcaatt gacgacaaac ggtctgagtc cttcctcaaa ccgacagtta tcgtctggga
    17341 aaatgtccca ggagtcctgt catcagcaga caacgcgttc ggatgtttcc ttgccggatt
    17401 ggctggagaa gatgcgccat tcgaacctgg tgatcgacct gaatcacgaa aaagtaacac
    17461 gttctggcgg tgggatgtca aaaccggttg ccatgctcca aagtggccgc agtgtggttg
    17521 tatttatgga ccgcagcgaa aggtggcctg gagaatcctt gatgcccaat acttcggagt
    17581 ggcacaacga cgccgacgcg tgtttgttgt cgcaagtgct cgaacagacc tcgatcccgc
    17641 aacggtactt tttgagttcg aaggcgtgcg ccgggatatt gcgccgagca gaggcgaggg
    17701 gaaggaagtt gccggaaatg ttggaaatgg cattaaaagc gggagccatt gggataaccc
    17761 tgtaaatcct cacccgacac ttaaccagtc acacaatact ggtgggattg gtgcaagtaa
    17821 ccaagagatt tttgcgcagc gaggcagcgg acttgtgtat tgcatggcgc atgggcaagg
    17881 gggggcagaa ataaaaaatg atgggacatc acctactctt acttgtaatc atgaagctcc
    17941 aattgtgttt tcttgcaaga aagctattgc gtttacaagc aaaaattatg ggcaggatgc
    18001 gggtgttgag atttccccaa ctttacgagc tggtaactct gctaatagca atcaaaacgc
    18061 gggatgccca ccagctgttg cgtattcgct acaacatgca caaattgggc gaaaagatga
    18121 tgctggaccg cagggtaaag ggtggcaaaa agagataagt tttactcttg attcccgcgc
    18181 aacagctgac gccgttgcgt tatctttttg ggggcaggaa agtattgaaa aaggcgaatt
    18241 aggtgagaat ggttacttac ctcatatgat gagcgttcgc cgtctgactc ctgtcgaatg
    18301 tgaaaggctg caaggttttc ctgatggaca tacgttgatt ccaacgggaa agcgtaagaa
    18361 agttacttca gatgaactta tataccttcg caatcacgat cctgatttaa gtgaagaaga
    18421 ggccgccatg cttgcagctg acggacctcg ttacaaagcg atcggcaata gtatggcgat
    18481 accagtaatg cgctggattg gcgagcggat taccaaggct gcatgtcggc agaaagaagg
    18541 aagtgaaaca aaagagcgaa aagttaaacc agcggcagaa ttcgaacggt ccatattcaa
    18601 atgggctggt ggaaaatttg gtgttctgga acaaatcttt cgctatttgc cagaagggaa
    18661 gcgcctgatt gaacctttcg ttggtggcgg agctgtcttc atgaatgccg gataccagga
    18721 aaatctgcta aatgatgtga atgctgacct gattaacttt tacaagactc tgcaacgcga
    18781 ggcgcattca cttatcactc tggcacatcg tttcttccag gactacaaca cgcaggaagg
    18841 atacctggca gtacggaatg cgtttaacaa acaagtctat gatgatttac atcgcgcagc
    18901 ggcgtttttg ttcctgaacc gacattgttt taacggattg acgcgttaca accaagccgg
    18961 tgagttcaat gtcggttatg ggaagtataa aactccgtat ttcccattgc aggagatgga
    19021 agccttcctt ggtgcggaag ggcgttctga gtttgtatgt ggtgattttg ctgcggtgat
    19081 tgaagctgcc ggagaaggag atgtcatctt ttgcgatccg ccgtatgaac cgctcccaaa
    19141 tacagaggga ttcacgaact attccggtca tgactttaag tttgaagagc aaaaacgcct
    19201 ggtgtctctg ttgacggatg ctcatcgccg aggtgcaaag gttctcatta ctaacagtgg
    19261 cgcgccaaac atcagagaac tttatcagga cagtggcttc agagtggaac ctctttttgc
    19321 cagacgttct gtgtcttgta agggggacac tcgaggtgtt gctcatgacg ttatagcaat
    19381 attgctataa taaatttatt agtgtaatat cgcctcaatg aatcgtgatt tatagagcga
    19441 tttagctgtt agccgcgaca ggcgcggcgg caagtatggc ggggtagtga ctccttcccc
    19501 ctcatgacgc cgagttgcca ggttgaccat acgcctaagt ggcaacaccg aagtgcgtta
    19561 cgagcttcca gtttgcccat cttcgggtgg gcgttttttt cagggttttc gtcatggtta
    19621 gcgactttgc ggcggtttag aaactgacca ttaaagtaaa tgcaaacgat gatctgatga
    19681 tggtagcggc ctaagaagcc agacgccacg gggtatgagt cgtcccccgt caaaaaatcg
    19741 accgcagagt gtccccgtct gtgtattagg gaacggggag gcacaacagg taagggcgct
    19801 ggtgtgatta accagatgaa cgagaagggg ccatctgttg gtcagcgtcc tttcctgttg
    19861 cgtcttcttt tcagcgtaac agcggtgctt aacagcactt tgggtacagt tccacgaatt
    19921 tacgggtata tcccgtcatg ctgaaggcgc taatcacgct ggaagccagg gttgtgcatc
    19981 ccctgttacc gaattgcagc cagggcgcgg tgcgccgaaa agcatacgga ggtggaagcc
    20041 ctcgccggag acgtacccgg caagtgatgg tgtagctcag cggttagagc ggttgactgt
    20101 taatcaacgg gtcgatggtt caaatccatc caccatcgcc aatgccggtt tagctcagtt
    20161 ggtagagcgc ctgccttgta agcaggatgt cagcggttcg agtccgttaa tcggcaccag
    20221 cacaacaggt aagggtattt tgcgacgtcg gagatcgccg agcttggcag agggttcgaa
    20281 tccctacgaa gtacccttac cgttgtgatg aatgcgcagg ctgatgcgcg aaagacattg
    20341 cagctattgc ggaaaagagc tgttcggcgg ggcaattaaa cgcccgtgag agtctgaaat
    20401 aaccgcaagc cggagatcag caccggtcat cacaacacaa caggtaaggg cattctccct
    20461 tatggggctt ggcttaaatg catcgagtgc tcttaccgtt gtgatgaagt gcagctcttt
    20521 gaagcaacca gaagataagc atctggcttc acaacataaa ccgcaggaac gaccaataaa
    20581 cggtagtccg tatggagaac accccgttga ggaagaggcc tggccggaac cgtaaccggc
    20641 actacaacgt tgagaacact ggcgtaacgg ggtcatatcc caatctacga ataaatgttg
    20701 cgttgcagcg tgacaaccag tgttctcaac attgtggtga atgcacaggc tgatgtgccg
    20761 caactacagt agtgcgcgct ttgcggggct tgctacaacc ctgtgtcgga gttcagcacc
    20821 gaccatcaca gtttgattct ctggcatgag cataacgctg aaataagtcc agtctggtgc
    20881 ggcccgatca cccgccgtta gctccacgaa acggagcacg taacaggtaa gagcattctc
    20941 ctgtaacggg ttcatatccc aatctacagg tccaccagga atgctctttc cgttgcggtg
    21001 aatgcggcta agcgcacgcg gggaaatggt tatatctgtc cattatttct ccttatttcc
    21061 ccgtccacgg tggataacca gccaaaggac accgggaggc acccggcacc gcaaccttat
    21121 ttcccaacca gtaatgaggt taataaatgc tcggcattct caaaaagaaa ttccgcaaag
    21181 cggctggcgg agtcaagaag atggaaaacc gtgatgcggt ggaagcgact gtctggggcg
    21241 catattccat tgcatactct gacggcacct gcgatgcgaa agaaattgca gtattggaaa
    21301 aaaccattgc agcacttcct gcctttgcgc cgttctccgg tgaaattgcc cagatgagcg
    21361 ccaatattcg cgctcaatat gaagcctcgc cgcgccgagc gaatgcccag gctttacgtg
    21421 aactggctga cgtgtctgga actaatgatg cggtagatgt tctgtgccta tgcattgata
    21481 ttgctgacca ggatggcatt ggggcagaag agcaggagca attgaagaaa attgcccagg
    21541 ctcttcaatt gccactggaa cagtatatct gatggttata aaagcacgtc taattctggc
    21601 tttggttttt ctcgtgctat ctgtgctggt ggatttcacc agcacaatcc tgtcggtttt
    21661 atccgacggg gcgttggtag cagtagctgt aacattggta tggccgatat ttaaaccagc
    21721 ctcgaaggat cagtgatgag cttctgggat tttgcagata agtatccaat tgttctcatt
    21781 atcattgttg ccatagttgt aggcggtatt gttagcgcca ttgaagcact taggaaacag
    21841 taatccggcc ctttagctca gtggttagag ctggcgactc ataatcgcac ggtcaccggt
    21901 tcaagtccgg taggggccac catatttggt tgtaacacgg cgtctggcac atgcgtcgtt
    21961 agcggtctgg tgacgttaaa aggggggaac cttgccccta gctcaggcaa cgaaccaggt
    22021 agccggaatg tgcaagccac cgtttattgt ttctcgggta aagggattca ccatcctggc
    22081 gattcggtgt gacagccggg aagagtccgg cgcattaatc ctgattttct ggtgatgact
    22141 catatcgtta ggagtgattt gagtatgccg attatatctg acattcagca cgcctgggtg
    22201 gagtgctaat gtctgcatcc cctcttgaat ccatgccaaa ttcccttagt gcagaacaag
    22261 ctgtacttgg tggcttaatg cttgataact gccgctggga tgaagttgca gatcgtatag
    22321 ttgctgatga tttttatacc agtgctcatc gtgaaatttt cagtgagatg gagaggttat
    22381 taagtcatgg caaaccgatt gatttgataa cacttgctga agcacttgaa cagaacggta
    22441 aattagaacg cgccggtggt tttgcgtacc ttgcggagat gtcaaagaac acgcccagcg
    22501 cggcaaatat ttgtgcttat gcggatatcg ttcgtgaacg cgcggttgtt cgtgaaatga
    22561 tttccgtcgc aaatgaaata gccgaagctg gatatgcgca ggatggcagg ggcagcaatg
    22621 aattgctgga tatggccgag cgccgcgttt ttgaaatagc tgaaaaacga caaaagagcg
    22681 gtagtggtcc aaaagatatc gccagcattc tcgatgcaac ggtatctcgc atagaagagt
    22741 tgtttcagcg accacatgat ggtgtaacgg ggcttgatac gggatttacc gatctcaata
    22801 agaagacggc aggacttcag gcgtccgatc tcattattgt cgccgcccgc ccatcgatgg
    22861 ggaagactac gtttgcgatg aatctcgtcg aaaatgccgc agtccgtaac gataagcccg
    22921 tattggtttt tagccttgag atgccgagcc accagctgat gatgcgctca ctggcttctc
    22981 ttgcacgcgt tgatcagtct cgtattcgaa cagggcaact taacgacgag gattgggcgc
    23041 gggtttctgg cgcaatgggt attctgttgg acaagcagaa tatttttatt gatgactcaa
    23101 gcgccctgac acctacagag cttcgttccc gcgctcgtcg tgtttataaa gaaaatggtg
    23161 gtttgagcat gattatgatc gactacctgc aacttatgcg cgtccccgag ctgcaagata
    23221 accgaacgct ggaaattgcc gagatttctc gctcactgaa ggcgttggcg aaggaattac
    23281 aagtaccggt ggtggcattg tcacaactta atcgatcgct tgaacagcgt gcggacaaac
    23341 gaccggtaaa ttcagattta cgtgaatcag gagcaattga gcaggacgca gacctgatca
    23401 tgtttctgta tcgcgacgaa gtttatcacc cggatagcga aatgaagggc attgccgagg
    23461 taattatcgg aaagcaacga aatggcccaa ttggcacggt gagattggct tttaacggcc
    23521 aatactcacg gtttgataac tatgccggtg ctgactggca agaggattat taatgcaatg
    23581 gaatgaggaa aagccgatga acatcctgat cattgggcga aaatttgaag ctatcagtga
    23641 tgtgaaaaca tatacggaaa tgtgggctta taacctggcc tgcgccttta gtgaggctgg
    23701 ggtaacattg caataccatc gtccatattc ccccggcgtc gaaagcccgg aggattatgt
    23761 tgaagctgtg ttgaccgctg cgacctcgtg ttctgcgaaa gccattttag cgccaggatt
    23821 gcggtatttt actacggtgc ccagggaaat aggcgtgcaa ctgcgtcgtc gattcactgg
    23881 atgggtagcc caggtatacg acggttctat gctggattcg gcaccagtcg atattacttt
    23941 tactgtccgc gatgatacct ggcggtacct ggataatccc ggcaggttag agcgtcataa
    24001 tcgctttaac aaacatgttg gatgggcagc gaatcaggag ctgttccatc tggaaaccaa
    24061 aacagacgat gttctgcgta tttttgtaga ccacgctgca tttgatgtta gtgggtttga
    24121 tcactcctta agtatcctta tgaaccttca gcgtctgacc gttccgtatg aggccagaac
    24181 gttgactgat gatggattgg ttaccattga tccggggaat atttcggtaa ctccatacag
    24241 acggacgccg gtgccagcaa ccgaatttgc agctgaattg cgtaagagtg acgtttttat
    24301 cgttacgcat cccgaaagcc ttggattaac tgttcttgag gcggcaatgt gcggggcgtt
    24361 ggtattaacg cctcccgatt gccttccgcc agatcgcctg gctttggtga accatatggt
    24421 tatcaagtcg cggattgatt gggatgaggt tattgctcgc gttgatcgcg tgaaaaatgc
    24481 tgaaaaggtc cagtgtcaca cctggtcggc aattgcggaa aagatgcttg agacgtttat
    24541 cacgcagaaa ccgtcgtgcg gtaacggata aaaattgaac ccattataaa agaaaagccc
    24601 gatcgccggg cttttcttaa gccttgtcaa cagagacttg agcggctttt atggatagat
    24661 tcccgctggc ctctatcgcc atacttcccc ccgccttcag ggcgacatcc gcgcctgact
    24721 ttatatcgag atttcctgcg gaagagatga atgccggacc ttgagaaatg gcatataact
    24781 ccccggcctc gttgaacccg attgttgttc cacttttcaa gtgcgtaacg gcccaggctc
    24841 cgcccgccgt ccggacctcc attagtccgt tccgcgacga aataaagtct tttttggcgc
    24901 tggttgatgg ttgtgctggt gcaccttcaa cttcaggcgg tacatagcct tcaccttgtc
    24961 ctgacgcttc aggtggcaca ttgggagcgc caccggatgc atcctgtgca taaccgatta
    25021 tcaatggcca tcgagaatcc ccattgtagg gaaattctac ccatacttta tcgccgggca
    25081 gaaatggcga aaacgtgttt gcattggaca atatggcttc tgcccacggc aatgaggcat
    25141 ctggtagccc atccatcatg ccgacaacgc gtatttgcgt acgcatcaga cctttagggt
    25201 catcgacgct taccactaca gcccgatact tccctgtcaa actacccatt caccactcct
    25261 aactgtgcac ggctgacaaa acggaagcgg tcttcgaaat gagtcacgga catcactatc
    25321 attttgtcag ggatagattc atcgagttct ccgtcacctg ccgtgttatg cacgacaatt
    25381 ttcagcgtcg tacccggagt tagcgcggca tttccttcca ccagcatatc gaggcggggg
    25441 agaatgaatt tgttgtagtt cgccagcgcg gtaggatcgg gattgctcgt aaatttaatg
    25501 gggtcttcct ggttacctga gtaaaccaca cctttggtca tgtcataact ggccattctg
    25561 taattgtggc ggcgctggta ttcataatcg gcattcagga tgttgaactg actaattgta
    25621 aatccggatg tgttgggatt ggcggactca taagtaagcg atggagcggc gtttgccatt
    25681 ttttccatac ttttaaaatt gatcgtcccc ctggatgccc agcacataga accggtatcc
    25741 cgggctatct cctgcaatac cttggtcggt ttttctccaa catttaggtg gtatgtggat
    25801 gtttttctga atgagtcagc attaaccttc agaccagggg caagagagga aactacggct
    25861 gatgggggct tatcaacaaa atactgtgcg ctggtggacg gaacttttaa taaccgcacc
    25921 gggttactaa acgcgtaaat cagtacagta tcgtccttgc gcggcgcttt aagaacaaag
    25981 aactcttccg agaagaggat gccgccatga ccttccggat caccaagtga aactgtcagt
    26041 attgtaccaa atttcacccc cagcttattg accacgtaag ccgttgaatc tctgaccatg
    26101 agcataagct ggggaccaga tagctccccg ggttcgacat aggtacatcc tacgatcatt
    26161 tcgcgaggga tttcgttctg cccaattgaa acagattgca ggaatagctg agtgcgtttt
    26221 gaatcagttt ccggggcggt ggtggtcttt gtggccatct cacgcctcca gaattttcgc
    26281 ttttaccgtt atggtgccgt tggtttgctt catataagcc aggattggaa gctccgccac
    26341 aacggtgagg ttcaatccaa ccgcgaacag cctgttatcg gcggtgccgg tggtcagatc
    26401 ctgaaatgcg attgattttt gcccttctat gtaacaggta accggtatct cataaccgcc
    26461 gacattggca atatgagtga aagatgcctg cccgaggctg gcatacattc gtagccagaa
    26521 tgctaatgca gttgaaacca tcccaagaga ttccttctcg tcactggcta tccatagcga
    26581 atattccagt gagaaaggga tagtcgatac cagggcttca atctcatcat tttcattggt
    26641 gacctgtcct tcctcgtaat tatcgcggca cagttcacct tcataaattg aaaacgcggg
    26701 agaacgagac agattcacaa gcggcattgc cagcttattt accgggccag aagagactgt
    26761 atctttacgc ccggcgcgat cggcttcaaa tgatgacaac cattctttca catcactaaa
    26821 agtgccgagc gttatgcgat ctcttggggt gcgtttcaga aactcccgga acgactggtt
    26881 agctcgatca ttaaagctga caacttgtga gtcgaacgct tcgtttaaag cctgtgcgag
    26941 cgccgaatca atgccatcaa tagtggcaaa ttccagctta ccggttggag taagaccttt
    27001 tttcttaaag atggccagta gccattcctg attattcaga atcaccgatg aaattccctt
    27061 caaaggcgcg tgaaggcacg caataaaaca aactgcctac cctggcagtg ccgtaattga
    27121 atattttatg gatgtaccag aagcggcgaa tggttgtgcc gtctgacagc tgttccagcc
    27181 attcgagcat agaacccact ggcacattaa cggcggctaa ccgaaggatt aaagcactgt
    27241 cgctaatccc tgtattatca ctgccgtcgt atagcgcgta gaaggcgtcc atctcatccg
    27301 ggcagtcgag ggccgttatc agttctggat cctgatagtc atatatgcgt tggttcggtt
    27361 ctattatttc agatgccgtt tcaggtgcat ttttgtctct gtaaggtatt gcgcgataca
    27421 gaactgcatc gaatgagtct gggtctagct tgattgcttt gagccagtcc atccgcacaa
    27481 ggttattaaa aactgcatga ccttgataac ggtggcgcac accagaatca ctaagcaggc
    27541 cgtgatccag attgggaagg tgattgtcct ccacaggatc aacaatatta ccaacgttaa
    27601 caccatcggt ttcgatttca gcatcaatat cttcctcttc aatcagttca gaaccttcgc
    27661 ctggaatatc cggatccgat tcggtgtccg ggaggttatc accagtcact tgttgtgatg
    27721 gttctgtgtc ctcaaacatg tcatcaaaga aacctgccat cgattatcct ttccgtttac
    27781 gggcttcgtt aatttgtgtc tcaagaatgc ttcgcgcctg cgcagtggca gcggccttgt
    27841 ccattccctg actcatgaaa aactttatga ggttgttcgc ctgcgtttgc agagcttttt
    27901 tgagagcgtc ggcttcagcg cgagcctggg cttccctcac ccgcgaggct tttagttcgg
    27961 cattcttcct gtttgccgtg gtgcgagctt tttttaacaa ccggcgaacg ttgtccgtgg
    28021 cgctatcttt ggcgcgtagt tttttgccta atgcatcctg agatttcaga tacagctcat
    28081 actcacgcgc agctttagcc tgatccgtcg ttgttgtccg gttgcgcgcg agcgatttag
    28141 ccagttcgcc tttgaaatag gttgttgtct tccgcttgtc atcgccgaag gccacctgtt
    28201 cagctgcttt ttccagggca ataatgatgg ccttgtgcca tgtgggagac tgaaaacgcg
    28261 tcatagcgtg caaaacatgt ttgcaagcca caccagtcag atcagggttg cggattttgg
    28321 ggaatgcata ctcttttggc ggcgcgacag catagttacc agccgtggcc atataacgat
    28381 accagtattg atggcgtcca caatcacagt cgaaagatac ccggcccttg cagagatcgg
    28441 cagcgattcg ggcttttttc gcaccgtctt cagcaatatc ctcaacggct ttatcccatt
    28501 cctcaaatcg aattctgaca cggtgatgct ggtggaccga ctcatccgag gcattaacag
    28561 atatcaatgc aaggttgtgt tttagcccga ggaatgtcgc ggctttgatc cctgtgccat
    28621 cagaaacttt gttgttagcg cgttttatat caatgctggt ggactgcgcc accagctgag
    28681 cataggtaat gccgggtacc gtgctcttga attttgtttt atgagcctgc cttgaggtgt
    28741 tgaaactgcg tatatcttcg ggcgtaaagt aggtgccatc tttctttttc ccaaggctga
    28801 ggaatgcctc aagttcgcgg ttacgcatcc ccataatcct tggggtgagt gtacgccgcg
    28861 cgtttcgccg attctgacgc tgctgtttac ggataagatc gaagaccttg ttaaagtctt
    28921 ttgcacttaa tccatcagtc tgatagcgac caaggttgtc gcgagcatat tcagttggca
    28981 ttcatttccc ttacgcaatg gataatgtcc ctatcacctg gccgtcatat tggaaatggc
    29041 gaatcatttc gcggatccat gtggcaggtg gtagttttaa ttttttgcca acagtcatac
    29101 cctgagactc atcctcaagc ccggcggcga gcgtcacaac ccagcgtagc tctgctatgc
    29161 cccacatacg gtaagccagc aaatccgggc gatattgctc atctggaaga acgtaataaa
    29221 tcgtcagatt cttgtcgttc gattcacaca taagcatcac ctctttgcgc agctctgccc
    29281 tgagtattgg atcggctatg ttgcggtcgt cataccgcga cagaggatat tgccgggtgc
    29341 tttgggttgt agtgattgat gtagccatag tcagcctgcc agaaatagat gatggtgatt
    29401 ctaccgctag tcatttgttg aatatttaac tcaataaaag aaaattatta gtgcaatttt
    29461 gattgtgaaa tgtatcattc tgcccttaag taggttcttc acgaggaaac aaaattggca
    29521 gaacgtgttg atgatgcaga gctgagcatg aatcagttag aagctctcaa agacatggcc
    29581 atcgataaca tcagaaagca ggcacaggtc gtgagccagg tttttacagg gaagtgtcgt
    29641 tactgcaatg aatcgattga atcaggcatt tattgtgacg ctgaatgtgc gctggcacag
    29701 ggaagagcag gctgcaaaac agcgtaaata tggcatgcga ccggcaggat ttgactgatt
    29761 atgttgcgct ttactgagga agagtttcag gcttttagtg agcgtcgaaa taaggggcgg
    29821 tccaggccaa aaaccaaaaa ggatccattc ttatcgcttg cgccggtaaa agaagtttct
    29881 ccacatgcga aggcacttgc agcactggca aagaacccag acctgcgcga cggaaattgc
    29941 gagcacttcg agcaggtttt catttttgat tacttcgaac gcaagcaccc tgacatctat
    30001 gagctgttgc atgcaacgcc taacggaggg aaacgttcaa aagcaaccgc cgggaaaatg
    30061 aaggctgaag ggcagaaaaa aggttatccg gacatgagtc tcgataaagc atgcggtatt
    30121 tatcacggca tgcgaattga gcttaaagaa ctaaatggta aagccccgac gaaagagcag
    30181 atcgcctgga tgcgcaggct tagagaggaa ggttactacg tcgttcttgc gtatggtgca
    30241 gaacaagcga taaccgccat cctggaatac ataagcctta aaaagggtga ggctattgag
    30301 catgtattga acggcgataa gtggttgcat gcttcttgaa ataataaatt aattagtgca
    30361 tatccgctct ttgtgatagt gcgccttaac atcgggagaa taatcgtgtc atccaaggtt
    30421 aattatgagt cgctggcatc ggtcatgccg cgtaatgaac aggaaacaga tgctgtagtg
    30481 gaccctgtaa tcgctgaaat gaatgctcgc ctggaggctg aatttgcagc tgagaatgaa
    30541 cataccaccc agggcgacta ggactgtttt ttgtgtcggt agcggtcctt cactcactcg
    30601 tgaggactgt gctgctatag aaaaaactgg ctgttcaatc atcgcggtta acaattcctg
    30661 gcagatgttc gatgacattt atgccttata cgccggtgat ttgtcatggt ggaagcaata
    30721 cggttccacc ataccgggag ggaggttccg caaagtgaca gccaacctgg cagcggcgaa
    30781 atcattttcg ttggagtaca ggcgatattg tggaccggcg gaaggggtaa atagcggcgc
    30841 gcaggctatc agtctggcgg ctgaatcagg ggctgaagta gttgtattag tcggctatga
    30901 ctgttctctg caaaacggcc ttcattggca tggggcgcac cctcaagcac tacggaatcc
    30961 aacgcaggta tctatttcaa aatggcaaca gcagttcctg gatacccgca aaaaacacgc
    31021 agatttacat attttgaatg caagtaggag cagtgcaatt caatgtttcc caagaataaa
    31081 tttagaggca gtgatcgcgt tattatcgtc ggcagtggcc caagcgccgc aaactttgtt
    31141 gcgccgcgcg gagtgccgat tatagcggtc aatggggcta tcgactggct taaccgcgct
    31201 tcttattttt tcacccttga tccatcccca gacaatatgc ggcgcgttgg tcgtggccgc
    31261 cgtcgccgtg gtgtttgtta ttgcatggca ctacccgatg ttaaagaacg tgaagtcaga
    31321 gatggcgttc tgtgcttccg tcgtgtggct gaacgcggca cggagccaaa aaatacgaat
    31381 tctcccgagt ggtgggcatg gcgctggtcc gcacatttcg gcctttgcga agatgagaat
    31441 gaaattgcca gcggcaatag tgcatatggt gctctgaacc tggctttcca tatcggattc
    31501 aaacatgtag ccctggtggg cgttgacgct acgcaagaac cacgcgttca ctccggcggc
    31561 acgccaaaaa atctaagtca cctgcctttg ttattccagt ctgcgcgtga acagattgac
    31621 gttgtttcat gcgggaaaat gggaggtatt ccgcagatga ctcttaaaga atggctgaag
    31681 aatacatgat ggcacccaca atttatcacc gtatcgacgg taccaaatac aggaatgtct
    31741 gggttgttgg tgatctgcat ggttgctaca ccagactgat gtccgaactc catcgtgtgg
    31801 attttgaccc ggcgcaggat ttactgatat cggtcggcga ccttatcgat cgcggtaccg
    31861 aaaatgtcga atgtctggaa ctattgcaga tgccctggtt cagggcagtg atggggaacc
    31921 atgagcggtt gatgctcgat gcgttaagtc ctgatggcaa cgtgaataac tggctaatga
    31981 atggtggaca atggttcttc atgttggaca ctgatcagga aatattagcc agggcgctgg
    32041 tggagctggt aagacgtctg ccctatatca ttgagttgaa caccgggcaa gaaactatcg
    32101 ttatagccca tgccgactat ccggataatg aatatcaatt cggtaaggag gtgccgcttt
    32161 tcaacgttgt ctgggcgcgc gagcgtatca gtgattcgat ggatgatatt ggtggcgaaa
    32221 tttcgggcgc agatcgtttt atctttggtc acactccggt gaaaagcccg aagacattct
    32281 ggaatcagca ttatatcgac actggtgccg tattttgcgg aaacctgaca ttgatcaaag
    32341 tgaaaggtga tggtgcagca tgaagattgc tttagttctt cgctctggtg gtgactataa
    32401 cgcttccgat gtgcagtggc tggttaatca actgccaaaa ggctatgaaa ttatttgcct
    32461 gacagacctg aagcgtttac atgtacctgg cgtcaaagtt atcccattga tcaaccagtg
    32521 gcaaaagtgc cgtggctggt gggcgaaaat cgagttgttc cgaccggata taaccgatga
    32581 tctgttctat ctggatttgg acacggttat tgccggtgat atacgcccaa tccttgagaa
    32641 tccaccaacc agcttcacca tgcttaggga tttttaccat ccacaatatc gtggtagcgg
    32701 tgccctgtgg ataccaaata gtgtaaaagc gcatatctgg agtgcattct ggcaagatcc
    32761 ggaaggttgg attgctcgtt gtgttactac tgaatgctgg ggtgatcagg ggtttttgcg
    32821 gaaggttatg ggtgatgata caccagcatt tcaggatctg tatccaggat ggtttgtaag
    32881 ttacaaggcc gatgttgtgg aacctggttc aaaatatgcg agcgcgcgtt actccagggg
    32941 gaatgggaca ttaccaaagg actgccgaat aatctttttc cacggcaaac cgcggcctcg
    33001 cgaagtgtca gaggattggc ttcccctcat tagctcgttt tttgagcgag aatcagaata
    33061 atattgctct aataattcca tatttttaaa acgtgatgta cactcatcac gttttttatt
    33121 agagcaatcc ataaggtgca ctatgtggcc attccgacgg aaatatcact actggctgat
    33181 cgcctttgtt acgccgaccg gcggtatcag gcatgtcatc accaggtatc gcaacaagag
    33241 actcacctta gccagaattt tacaggctgc cataggtgag ggactggata caaattgcgt
    33301 agtccttcct ccttcatact taggaaaaat gaccgaagca caagctaata cggaactttg
    33361 aaatgagcac ttcagcacaa aaccaatcaa tcgaaaatgt atctatccct gatgtcctga
    33421 atgccggtat cccggccatt atccagaaca tccgggccgc gcaacgccgc gttagttgtg
    33481 atgacctcac agcacgtttt tttgataatg cggttcagtc agcggagatg cttcacgcac
    33541 agcttattga tgtttataac gcagaagctg atagccataa ctccctggta gatgcagctg
    33601 aaaatatgca gttggatctc ggtctgaagg gtaaagaaat tgaagagctt cagctggaaa
    33661 ttgaacattt gaaacgccag caacaggacg cgatcgacga tgcgacgcat gacgccaacc
    33721 agcgtgctga taatgccgaa cgtataagca ttgagctgga aacaaaactc aatgaaatga
    33781 ccgcgatggt tgaactgcgg aactcacaga tttcaacgct aaaatctcaa tataaagaga
    33841 tcatgaaact tgatcctttt aaccttgaga aacgctataa caaagctaaa agcgagcgac
    33901 aggaactgcg taagcaggtc gccgacctta accaacagct caaaaaaact attaaagatg
    33961 caagcgaggc gcgcgtggca tttgctaata aaaaagcaga ggttaccgcg ctggttaatg
    34021 agaatgccaa atttgcgacg ctcaagaagg aaatgtatgg cattactgag cgccgtttcc
    34081 ctgcaagcaa actccatccg acgttagggc aaatctcatt cttcccgcgc ctcctggctt
    34141 atggaatctc atcgcctaaa gagttcaata acgagcgtcc ttatatcgtt tctaagctgg
    34201 actttgctta tcagttctgc tgcgacatgg gctatgccat tgatatccgt atcaacgaat
    34261 ggttgatgcc aaacttccag ccgttggcca ttttccgcga gttccagccg gaaggttggg
    34321 tagagttctt ccatgaaatg atctgtaaag agatggaaag ccgccgcccg gaactggtcc
    34381 gccgtgtcga gtgggcgcaa gaggttatgt tggcagatgc agagctaccg ttcgaaccgg
    34441 aattcattga tgaactggcc aataaagggc tgcatagcct atttgatgta gttacccgcc
    34501 gtcatgagca gttggttgtt gaattgggtt tagaggaaac tgcggcaaga agacttctcg
    34561 atgtttgcta tgcacgtagc gatgcatggg aaaaagagaa cggcggcact atttacgttc
    34621 gctgatagtt acagtgtcac ttttaatgct ggtggagtgc gcccaccagc atttttttcg
    34681 tccaatgagg agggcatttg agtattttca ataaacacgc acaccaggaa cgtccgtaca
    34741 tcgtcatagt agatattgat gggacgatat cagaggcaac ggaagacagg ctgcatttac
    34801 ttccaccacc tggcaaaggt gcattaacag agcactggaa cgagtttaac cttgcctgtg
    34861 acactgatgc tcccatcact ccagttattg atatggtgcg ccagttgtcc ggcatttaca
    34921 ccctctggtt tgtaaccggg cgctgtgaga tagccaggga taaaacacga gcctggttgc
    34981 gtaagcacgt aacaaatggg gctgagcctt tgctatctat gcgtcctgcc accgatgaca
    35041 gaaatgacgg tccagcaaag attgatctcc tgaagaaaat tggtctaagt aaaattgcgt
    35101 tcgcgctgga agataagatt gaagtggcgc gtgttttcag gagtcacggc gtacttacgt
    35161 taatggtcag ggagtatgaa aacgcgcttc tccatcaaca ataattgctc taataaatct
    35221 tgatttttaa aacagagaaa gtgaaaataa aaacatgccg caaggcgcgg catgtatcca
    35281 atcaatcaca ggagctgaaa atatgaacac ggcattcaaa atcattatgg ccgcgatcta
    35341 tttctggctg ttctctatca cttttggcgg catcgtcgcg catgggtaag ggggatgcat
    35401 gaaaggcgaa gtgaaagagc gcggcatgat tttcaacgat gagatggtcc gagccatcct
    35461 cgaaggaagg aaaacgcaga cgcggcggat aatgaaaaat cagcctgcgg aagttggtcc
    35521 agaagcacca gtgatggtta gggaaattgg tgcaggtttt cagtggtacg gggctgatgg
    35581 tgtaagcagt gttttcaatt gcccctttgg tatcgtcggc gatcgaattt gggttcgtga
    35641 aacatgggcg atattaggca atgaagatgg ttgcagtgtg gactggaacg acaacctttg
    35701 tcgtggcgat gagaagaacg cagcaaggat ttatcgggcc agttgtgagc aaaagcctgg
    35761 tgattacggt ttatggtcga tacccgatga tgccgactgg aaacctcaca ctgtgaatga
    35821 aaagtttgat ggtgggtggc gtccatcaat tcacatgccg cgatgggcat cacgcatcct
    35881 gttggaaatt accaacgtgc gcgttgagcg gttgaacaat atcagcgaat gcgatgcaag
    35941 ggatgagggc gttccgcctg ctggaagttt gcttcctgat cacccgggaa cattccttac
    36001 tcccaagggg gatttcgcaa tggccaaggt tgcgttccag cgcctgtggg aatccatcta
    36061 tggtgaagaa agctggaacg ctaatccctg ggtgtgggta atcgagtttg agcgtattca
    36121 gtagggcgag cgtatgcaat ctgttatttg tgaggaagtc ggcctgaata aagcatcccc
    36181 agcagagtta agagcaagtc tcgaaatggc gcatagcctt gctcaaattg gtgtcaggtt
    36241 tgtaccaatt ccagttgaaa cagatgaaga atttcacagg ttagcggcat cggcagcaca
    36301 aaagctggaa atcatggcag cgaaagtag
//
LOCUS       DAMZFT010000035        36049 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.035, whole genome shotgun sequence.
ACCESSION   DAMZFT010000035 DAMZFT010000000
VERSION     DAMZFT010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 36049)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36049)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36049
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.035"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            1..1860
                     /locus_tag="PN977_001865"
                     /pseudo
     CDS             1..1860
                     /locus_tag="PN977_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309023.2"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC family protein"
     gene            1897..3645
                     /gene="msbA"
                     /locus_tag="PN977_001866"
     CDS             1897..3645
                     /gene="msbA"
                     /locus_tag="PN977_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A ABC transporter ATP-binding
                     protein/permease MsbA"
                     /protein_id="HDI6041897.1"
                     /translation="MHNDKDLSTWQTFRRLWPTIAPFKAGLIVAGVALILNAASDTFM
                     LSLLKPLLDDGFGKTDRSVLVWMPLVVIGLMILRGITSYVSSYCISWVSGKVVMTMRR
                     RLFGHMMGMPVSFFDKQSTGTLLSRITYDSEQVASSSSGALITVVREGASIIGLFIMM
                     FYYSWQLSIILIVLAPIVSIAIRVVSKRFRNISKNMQNTMGQVTTSAEQMLKGHKEVL
                     IFGGQEVETKRFDKVSNRMRLQGMKMVSASSISDPIIQLIASLALAFVLYAASFPSVM
                     DSLTAGTITVVFSSMIALMRPLKSLTNVNAQFQRGMAACQTLFTILDSEQEKDEGKRV
                     IERATGDVEFRNVTFTYPGRDVPALRNINLKIPAGKTVALVGRSGSGKSTIASLITRF
                     YDIDEGEILMDGHDLREYTLASLRNQVALVSQNVHLFNDTVANNIAYARTEQYSREQI
                     EEAARMAYAMDFINKMDNGLDTVIGENGVLLSGGQRQRIAIARALLRDSPILILDEAT
                     SALDTESERAIQAALDELQKNRTSLVIAHRLSTIEKADEIVVVEDGVIVERGTHNDLL
                     EHRGVYAQLHKMQFGQ"
     gene            3642..4628
                     /gene="lpxK"
                     /locus_tag="PN977_001867"
     CDS             3642..4628
                     /gene="lpxK"
                     /locus_tag="PN977_001867"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HDI6041898.1"
                     /translation="MIEKIWSGESPLWRLLLPLSWLYGLVSGAIRLCYKLKLKRAWRA
                     PVPVVVVGNLTAGGNGKTPVVVWLVEQLQQRGICVGVVSRGYGGKAESYPLLLSADTT
                     TAQAGDEPVLIYQRTDAPVAVSPVRSDAIKAILAQHPDVQIIVTDDGLQHYRLARDVE
                     IVVIDGVRRFGNGWWLPAGPMRERAGRLKSVDAVIVNGGVPRSGEIPMHLLPGQAVNL
                     RTGTRCDVAQLEHVVAMAGIGHPPRFFATLKMCGVQPEKCVPLADHQSLNHADVSALV
                     SAGQTLVMTEKDAVKCRAFAEENWWYLPVDAQLSGDEPAKLLAQLTSLASGN"
     gene            4665..5897
                     /locus_tag="PN977_001868"
     CDS             4665..5897
                     /locus_tag="PN977_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcaQ family DNA glycosylase"
                     /protein_id="HDI6041899.1"
                     /translation="MSLPHLSLADARNLHLAAQGLLNKPRRRASLEDIPATISRMSLL
                     QIDTINIVARSPYLVLFSRLGNYPAQWLDESLARGELMEYWAHEACFMPRSDFRLIRH
                     RMLAPEKMGWKYKDAWMQEHEVEIAQLIQHIHDKGPVRSADFEHPRKGASGWWEWKPH
                     KRHLEGLFTAGKVMVIERRNFQRVYDLTHRVMPDWDDERDLVSQTEAEIIMLDNSARS
                     LGIFREQWLADYYRLKRPALAAWREARAELQQIIAVHVEKLGNLWLHADLLPLLERAL
                     AGKLTATHSAVLSPFDPVVWDRKRAEQLFDFSYRLECYTPAPKRQYGYFVLPLLHRGQ
                     LVGRMDAKMHRQTGILEVISLWLQEGIKPTTTLQKGLRQAITDFANWQQATRVTLGRC
                     PQGLFTDCRTGWEIDPVA"
     gene            5949..6131
                     /gene="ycaR"
                     /locus_tag="PN977_001869"
     CDS             5949..6131
                     /gene="ycaR"
                     /locus_tag="PN977_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YcaR"
                     /protein_id="HDI6041900.1"
                     /translation="MDHRLLEIIACPVCNGKLWYNQEKQELICKLDNLAFPLRDGIPV
                     LLETEARVLTADESKS"
     gene            6128..6874
                     /gene="kdsB"
                     /locus_tag="PN977_001870"
     CDS             6128..6874
                     /gene="kdsB"
                     /locus_tag="PN977_001870"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HDI6041901.1"
                     /translation="MSFVVIIPARYASTRLPGKPLVDINGKPMIVHVLERARESGAER
                     IIVATDHEDVARAVEAAGGEVCMTRADHQSGTERLAEVVEKCAFSDDTVIVNVQGDEP
                     MIPATIIRQVADNLAQRQVGMATLAVPIHNAEEAFNPNAVKVVLDAEGYALYFSRATI
                     PWDRDRFAKGLETVGDNFLRHLGIYGYRAGFIRRYVNWQPSPLEHIEMLEQLRVLWYG
                     EKIHVAVAQEVPGTGVDTPEDLERVRAEMR"
     gene            7028..7921
                     /locus_tag="PN977_001871"
     CDS             7028..7921
                     /locus_tag="PN977_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcbJ family phosphotransferase"
                     /protein_id="HDI6041902.1"
                     /translation="MEQLRAELSHLLGEKLSRIECVNEKADTALWALYDSQGNPMPLM
                     ARSFSTPGKARQLAWKTTMLARSGTVRMPTIYGVMTHEEHPGPDVLLLERMRGVSVEA
                     PARTPERWEQLKDQIVEALLAWHRQDSRGCVGAVDNTQENFWPSWYRQHVEVLWTTLN
                     QFNNTGLTMQDKRILFRTRECLPALFEGFNDNCVLIHGNFCLRSMLKDSRSDQLLAMV
                     GPGLMLWAPREYELFRLMDNSLAEDLLWSYLQRAPVAESFIWRRWLYVLWDEVAQLVN
                     TGRFSRRNFDLASKSLLPWLA"
     gene            complement(7898..8677)
                     /gene="elyC"
                     /locus_tag="PN977_001872"
     CDS             complement(7898..8677)
                     /gene="elyC"
                     /locus_tag="PN977_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope biogenesis factor ElyC"
                     /protein_id="HDI6041903.1"
                     /translation="MLFTLKKVIGNMLLPLPLMLLIIGAGLALLWFSRFQKTGKIFIS
                     IGWLALLLLSLQPVADRLLRPIESTYPTWNNSQKVDYIVVLGGGYTWNPQWAPSSNLI
                     NNSLPRLNEGIRLWRENPGSKLIFTGGVAKTNTVSTAEVGARVAQSLGVPREQIITLD
                     LPKDTEEEAAAVKQAIGDAPFLLVTSASHLPRAMIFFQQEGLNPLPAPANQLAIDSPL
                     NPWERAIPSPVWLMHSDRVGYETLGRIWQWLKGSSGEPRQE"
     gene            8813..9598
                     /gene="cmoM"
                     /locus_tag="PN977_001873"
     CDS             8813..9598
                     /gene="cmoM"
                     /locus_tag="PN977_001873"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine 5-oxyacetic acid(34)
                     methyltransferase CmoM"
                     /protein_id="HDI6041904.1"
                     /translation="MQDRNFDDIAEKFSRNIYGTTKGQLRQAILWQDLDRVLAEMGPQ
                     KLRVLDAGGGEGQTAIKMAERGHQVILCDLSAQMIDRAKQAAEAKGVSDNMQFIHCAA
                     QDVASHLETPVDLILFHAVLEWVADPRSVLQTLWSVLRPGGVLSLMFYNAHGLLMHNM
                     VAGNFDYVQAGMPKKKKRTLSPDYPRDPAQVYLWLEEAGWQIMGKTGVRVFHDYLREK
                     HQQRDCYEALLELETRYCRQEPYITLGRYIHVTARKPQSKDKV"
     gene            9595..10917
                     /gene="mukF"
                     /locus_tag="PN977_001874"
     CDS             9595..10917
                     /gene="mukF"
                     /locus_tag="PN977_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukF"
                     /protein_id="HDI6041905.1"
                     /translation="MSEFSQTVPELVAWARKNDFSISLPVDRLSFLLAVATLNGERLD
                     GEMSEGELVDAFRHVSDAFEQTSETIGVRANNAINDMVRQRLLNRFTSEQAEGNAIYR
                     LTPLGIGITDYYIRQREFSTLRLSMQLSIVAGELKRAADAAEEGGDEFHWHRNVYAPL
                     KYSVAEIFDSIDLTQRLMDEQQQQVKDDIAQLLNKDWRAAISSCELLLSETSGTLREL
                     QDTLEAAGDKLQANLLRIQDATMTHDDLHFVDRLVFDLQSKLDRIISWGQQSIDLWIG
                     YDRHVHKFIRTAIDMDKNRVFAQRLRQSVQTYFDEPWALTYANADRLLDMRDEEMALR
                     DEEVTGELPEDLEYEEFNEIREQLAAIIEEQLAVYKTRQVPLDLGLVVREYLSQYPRA
                     RHFDVARIVIDQAVRLGVAQADFTGLPAKWQPINDYGAKVQAHVIDKY"
     gene            10898..11602
                     /gene="mukE"
                     /locus_tag="PN977_001875"
     CDS             10898..11602
                     /gene="mukE"
                     /locus_tag="PN977_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukE"
                     /protein_id="HDI6041906.1"
                     /translation="MSSTNIEQVMPVKLAQALANPLFPALDSALRSGRHIGLDELDNH
                     AFLMDFQEYLEEFYARYNVELIRAPEGFFYLRPRSTTLIPRSVLSELDMMVGKILCYL
                     YLSPERLANEGIFTQQELYDELLTLADEAKLLKLVNNRSTGSDVDRQKLQEKVRSSLN
                     RLRRLGMVWFMGHDSSKFRITESVFRFGADVRAGDDPREAQRRLIRDGEAMPIENHLQ
                     LNDETEENQPDSGEEE"
     gene            11602..16062
                     /gene="mukB"
                     /locus_tag="PN977_001876"
     CDS             11602..16062
                     /gene="mukB"
                     /locus_tag="PN977_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukB"
                     /protein_id="HDI6041907.1"
                     /translation="MIERGKFRSLTLINWNGFFARTFDLDELVTTLSGGNGAGKSTTM
                     AAFVTALIPDLTLLHFRNTTEAGATSGSRDKGLHGKLKAGVCYSMLDTINSRHQRVVV
                     GVRLQQVAGRDRKVDIKPFAIQGLPMSVQPTQLVTETLSERQARVLPLNELKDKLEAM
                     EGVQFKQFNSITDYHSLMFDLGIIARRLRSASDRSKFYRLIEASLYGGISSAITRSLR
                     DYLLPENSGVRKAFQDMEAALRENRMTLEAIRVTQSDRDLFKHLISEATNYVAADYMR
                     HANERRVHLDKALEFRRELHTSRQQLAAEQYKHVDMARELAEHNGAEGDLEADYQAAS
                     DHLNLVQTALRQQEKIERYEADLDELQIRLEEQNEVVAEAIERQEENEARAEAAELEV
                     DELKSQLADYQQALDVQQTRAIQYNQAIAALNRAKELCHLPDLTADSAAEWLETFQAK
                     ELEATEKMLSLEQKMSMAQTAHSQFEQAYQLVVAINGPLARNEAWDVARELLREGVDQ
                     RHLAEQVQPLRMRLSELEQRLREQQEAERLLADFCKRQGKNFDIDELEALHQELEARI
                     ASLSDSVSNAREERMTLRQEQEQLQSRIQSLMRRAPVWLAAQNSLNQLSEQCGEEFSS
                     SQDVTEYLQQLLEREREAIVERDEVGARKNAVDEEIERLSQPGGSEDQRLNALAERFG
                     GVLLSEIYDDVSLEDAPYFSALYGPSRHAIVVPDLSQVTEHLEGLTDCPEDLYLIEGD
                     PQSFDDSVFSVDELEKAVVVKIADRQWRYSRFPEVPLFGRAARESRIESLHAEREVLS
                     ERFATLSFDVQKTQRLHQAFSRFIGSHLAVAFESDPEAEIRQLNSRRVELERALSNHE
                     NDNQQQRIQFEQAKEGVTALNRILPRLNLLADDSLADRVDEIRERLDEAQEAARFVQQ
                     FGNQLAKLEPIVSVLQSDPEQFEQLKEDYAYSQQMQRDARQQAFALTEVVQRRAHFSY
                     SDSAEMLSGNSDLNEKLRERLEQAEAERTRAREALRGHAAQLSQYNQVLASLKSSYDT
                     KKELLNDLQRELQDIGVRADSGAEERARIRRDELHAQLSNNRSRRNQLEKALTFCEAE
                     MDNLTRKLRKLERDYFEMREQVVTAKAGWCAVMRMVKDNGVERRLHRRELAYLSADDL
                     RSMSDKALGALRLAVADNEHLRDVLRMSEDPKRPERKIQFFVAVYQHLRERIRQDIIR
                     TDDPVEAIEQMEIELSRLTEELTSREQKLAISSRSVANIIRKTIQREQNRIRMLNQGL
                     QNVSFGQVNSVRLNVNVRETHAMLLDVLSEQHEQHQDLFNSNRLTFSEALAKLYQRLN
                     PQIDMGQRTPQTIGEELLDYRNYLEMEVEVNRGSDGWLRAESGALSTGEAIGTGMSIL
                     VMVVQSWEDESRRLRGKDISPCRLLFLDEAARLDARSIATLFELCERLQMQLIIAAPE
                     NISPEKGTTYKLVRKVFQNTEHVHVVGLRGFAPQLPETLPGSDEAPSQAS"
     gene            16323..18170
                     /gene="ldtD"
                     /locus_tag="PN977_001877"
     CDS             16323..18170
                     /gene="ldtD"
                     /locus_tag="PN977_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HDI6041908.1"
                     /translation="MLLNMMCGRRLSAISLCVAVTFAPLFNAQADEPEVIPGDSPVAV
                     SEQGEALPQAQATAIMAGIQPLPEGAAEKARTQIESQLPAGYKPVYLNQLQLLYAARD
                     MQPMWENRDAVKAFQQQLAEVAIAGFQPQFNKWVELLTDPGVNGMARDVVLSDAMMGY
                     LHFIANIPVKGTRWLYSSKPYALSTPPLSVINQWQLALDKGQLPTFVAGLAPQHPQYA
                     AMHESLLALLSDTKPWPQLTGKATLRPGQWSNDVPALREILQRTGMLDGGPKITLPGD
                     DTPTDAVVSPSAVTVETAETKPMDKQTTSRSKPAPAVRAAYDNELVEAVKRFQAWQGL
                     GADGAIGPATRDWLNVTPAQRAGVLALNIQRLRLLPTELSTGIMVNIPAYSLVYYQNG
                     NQVLDSRVIVGRPDRKTPMMSSALNNVVVNPPWNVPPTLARKDILPKVRNDPGYLESH
                     GYTVMRGWNSREAIDPWQVDWSTITASNLPFRFQQAPGPRNSLGRYKFNMPSSEAIYL
                     HDTPNHNLFKRDTRALSSGCVRVNKASDLANMLLQDAGWNDKRISDALKQGDTRYVNI
                     RQSIPVNLYYLTAFVGADGRTQYRTDIYNYDLPARSSSQIVSKAEQLIR"
     gene            18351..18899
                     /locus_tag="PN977_001878"
     CDS             18351..18899
                     /locus_tag="PN977_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcbK family protein"
                     /protein_id="HDI6041909.1"
                     /translation="MDKFDANRRKLLALGGVALGAAILPTPAFATLSTPRPRILTLNN
                     LHTGESIKAEFFDGRGYIQEELAKLNHFFRDYRANKIKSIDPGLFDQLYRLQGLLGTR
                     KPVQLISGYRSIDTNNELRARSRGVAKKSYHTKGQAMDFHIEGIALSNIRKAALSMRA
                     GGVGYYPRSNFVHIDTGPARHW"
     gene            18926..19573
                     /gene="gloC"
                     /locus_tag="PN977_001879"
     CDS             18926..19573
                     /gene="gloC"
                     /locus_tag="PN977_001879"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003035823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase GloC"
                     /protein_id="HDI6041910.1"
                     /translation="MNYRIIPVTAFSQNCSLIWCEQTRLAALVDPGGDAEKIKQEVDA
                     SGLTLMQILLTHGHLDHVGAAAELAQHYGVPVFGPEKEDEFWLQGLPAQSRMFGLEEC
                     QPLTPDRWLNEGDTISIGNVTLQVLHCPGHTPGHVVFFDERARLLISGDVIFKGGVGR
                     SDFPRGDHNQLISSIKDKLLPLGDDVTFIPGHGPLSTLGYERLHNPFLQDEMPVW"
     gene            complement(19623..20813)
                     /gene="aspC"
                     /locus_tag="PN977_001880"
     CDS             complement(19623..20813)
                     /gene="aspC"
                     /locus_tag="PN977_001880"
                     /EC_number="2.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009653304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate transaminase"
                     /protein_id="HDI6041911.1"
                     /translation="MFENITAAPADPILGLADLFRADERPGKINLGIGVYKDETGKTP
                     VLTSVKKAEQYLLENETTKNYLGIDGIPEFGRCTQELLFGKGSALINDKRARTAQTPG
                     GTGALRVAADFLAKNTSVKRVWVSNPSWPNHKSVFNSAGLEVREYAYYDAENHTLDFD
                     ALINSLNEAQAGDVVLFHGCCHNPTGIDPTLEQWQTLAQLSVEKGWLPLFDFAYQGFA
                     RGLEEDAEGLRAFAAMHKELIVASSYSKNFGLYNERVGACTLVAADSETVDRAFSQMK
                     AAIRANYSNPPAHGASVVATILSNDALRAIWEQELTDMRQRIQRMRQLFVNTLQEKGA
                     NRDFSFIIKQNGMFSFSGLTKEQVLRLREEFGVYAVASGRVNVAGMTPDNMAPLCEAI
                     VAVL"
     gene            complement(20998..22071)
                     /gene="ompF"
                     /locus_tag="PN977_001881"
     CDS             complement(20998..22071)
                     /gene="ompF"
                     /locus_tag="PN977_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpF"
                     /protein_id="HDI6041912.1"
                     /translation="MMKRNILAVIVPALLVAGTANAAEIYNKDGNKVDLYGKAVGLHY
                     FSKDNGVNSYGGNGDKTYARLGFKGETQINSDLTGYGQWEYNFQGNNSEGADAQKGNK
                     TRLAFAGLKFADAGSIDYGRNYGVVYDALGYTDMLPEFGGDTAYSDDFFVGRVGGVAT
                     YRNSNFFGLVDGLNFAVQYLGKNERAGIPERSNGDGVGGSISYEFEGFGIVGAYGAAD
                     RTDAQEAEFRGQGKKAEQWATGLKYDANNIYLAANYGETRNATPIEGGFANKTQDVLL
                     VAQYQFDFGLRPSIAYTKSKAKDVEGIGDVDLVNYFEVGATYYFNKNMSTYVDYIINQ
                     IDSDNKLGVGSDDTVAVGIVYQF"
     gene            complement(22673..24073)
                     /gene="asnS"
                     /locus_tag="PN977_001882"
     CDS             complement(22673..24073)
                     /gene="asnS"
                     /locus_tag="PN977_001882"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HDI6041913.1"
                     /translation="MSVVPVADVLQGRVAVDSEVTVRGWVRTRRDSKAGISFLAVYDG
                     SCFDPVQAVINNSLPNYNEDVLRLTTGCSVIVTGKVVASPGQGQQFEIQASKVEVAGW
                     VEDPDTYPMAAKRHSIEYLREVAHLRPRTNLIGAVARVRHTLAQALHRFFNEQGFFWV
                     STPLITASDTEGAGEMFRVSTLDLENLPRNDQGKVDFDKDFFGKESFLTVSGQLNGET
                     YACALSKIYTFGPTFRAENSNTSRHLAEFWMLEPEVAFANLNDIAGLAEAMLKYVFKA
                     VLEERADDMKFFAERVDKDAVSRLERFIEADFAQVDYTDAVTILENCGRKFENPVYWG
                     VDLSSEHERYLAEEHFKAPVVVKNYPKDIKAFYMRLNEDGKTVAAMDVLAPGIGEIIG
                     GSQREERLDVLDERMLEMGLNKEDYWWYRDLRRYGTVPHSGFGLGFERLIAYVTGVQN
                     VRDVIPFPRTPRNASF"
     gene            complement(24241..25443)
                     /gene="pncB"
                     /locus_tag="PN977_001883"
     CDS             complement(24241..25443)
                     /gene="pncB"
                     /locus_tag="PN977_001883"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HDI6041914.1"
                     /translation="MTQFASPVLHSLLDTDAYKLHMQQAVFHHYYDVHVAAEFRCRGD
                     DLLGIYADAIREQVQAMQHLRLQDDEYQWLSALPFFQADYLNWLREFRFNPEQVTVSN
                     DNGKLDIRLSGPWREVILWEVPLLAVISEMVHRYRSPQTDVAQALDTLESKLVDFSAL
                     TAGLDMSRFHLMDFGTRRRFSREVQETIVKRLQQESWFVGTSNYDLARRLSLTPMGTQ
                     AHEWFQAHQQISPDLANSQRAALAAWLEEYPDQLGIALTDCITMDAFLRDFGVEFASR
                     YQGLRHDSGDPVEWGEKAIAHYEKLGIDPQSKTLVFSDNLDLRKAVELYRHFSSRVQL
                     SFGIGTRLTCDIPQVKPLNIVIKLVECNGKPVAKLSDSPGKTICHDKAFVRALRKAFD
                     LPHIKKAS"
     gene            25709..28321
                     /gene="pepN"
                     /locus_tag="PN977_001884"
     CDS             25709..28321
                     /gene="pepN"
                     /locus_tag="PN977_001884"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000193787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HDI6041915.1"
                     /translation="MTQQPQAKYRHDYRAPDYQITDIDLTFDLDAQKTVVTAVSQAVR
                     HGASDAPLRLDGEDLKLVSVHINDEPWTAWKEEEGALVISNLPERFTLKIVNEISPAT
                     NTALEGLYQSGDALCTQCEAEGFRHITYYLDRPDVLARFTTKIIADKTQYPFLLSNGN
                     RVAQGELENGRHWVQWQDPFPKPCYLFALVAGDFDVLRDTFTTRSGREVALELYVDRG
                     NLDRAPWAMTSLKNSMKWDEERFGLEYDLDIYMIVAVDFFNMGAMENKGLNIFNSKYV
                     LARTDTATDKDYLDIERVIGHEYFHNWTGNRVTCRDWFQLSLKEGLTVFRDQEFSSDL
                     GSRAVNRINNVRTMRGLQFAEDASPMAHPIRPDMVIEMNNFYTLTVYEKGAEVIRMIH
                     TLLGEENFQKGMQLYFERHDGSAATCDDFVQAMEDASNVDLSHFRRWYSQSGTPVVTV
                     KDDYNPETEQYTLTISQRTPATPDQAEKQPLHIPFAIELYDNEGKVIPLQKGGHPVNS
                     VLNVTQAEQTFVFDNVYFQPVPALLCEFSAPVKLEYKWSDQQLTFLMRHARNDFSRWD
                     AAQSLLATYIKLNVARHQQGQPLSLPVHVADAFRAVLLDEKIDPALAAEILTLPSVNE
                     MAELFDIIDPIAIAEVREALTRTLATELADELLAIYNANYQSEYRVEHEDIAKRTLRN
                     ACLRFLAFGETHLADVLVSKQFHEANNMTDALAALSAAVAAQLPCRDALMQEYDDKWH
                     LDGLVMDKWFILQATSPAANVLETVRGLLQHRSFTMSNPNRIRSLIGAFAGSNPAAFH
                     AEDGSGYQFLVEMLTDLNSRNPQVASRLIEPLIRLKRYDAKRQEKMRAALEQLKGLEN
                     LSGDLYEKITKALA"
     gene            complement(28364..29131)
                     /gene="ssuB"
                     /locus_tag="PN977_001885"
     CDS             complement(28364..29131)
                     /gene="ssuB"
                     /locus_tag="PN977_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonates ABC transporter ATP-binding
                     protein"
                     /protein_id="HDI6041916.1"
                     /translation="MNTARLNQGTPLLLNAVSKHYAENIVLNQLDLHIPAGQFVAVVG
                     RSGGGKSTLLRLLAGLETPTAGDVLAGTTPLAEIQDDTRMMFQDARLLPWKSVIDNVG
                     LGLKGQWRDAARQALAAVGLENRAGEWPAALSGGQKQRVALARALIHRPGLLLLDEPL
                     GALDALTRLEMQDLIVSLWQKHGFTVLLVTHDVSEAVAMADRVLLIEEGKIGLDLTVD
                     IPRPRRLGSVRLAELESEVLQRVMQRGESEIRLRKQG"
     gene            complement(29128..29919)
                     /gene="ssuC"
                     /locus_tag="PN977_001886"
     CDS             complement(29128..29919)
                     /gene="ssuC"
                     /locus_tag="PN977_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415454.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter permease
                     SsuC"
                     /protein_id="HDI6041917.1"
                     /translation="MATPVKKWLLRVAPWFLPVGIVAVWQLASSVGWLSTRILPSPEG
                     VVMAFWTLSASGELWQHLAISSWRALIGFSIGGSLGLILGLISGLSRWGERLLDTSIQ
                     MLRNVPHLALIPLVILWFGIDESAKIFLVALGTLFPIYINTWHGIRNIDRGLVEMARS
                     YGLSGIPLFIHVILPGALPSIMVGVRFALGLMWLTLIVAETISANSGIGYLAMNAREF
                     LQTDVVVVAIILYALLGKLADVSAQLLERLWLRWNPAYHLKEATV"
     gene            complement(29931..31076)
                     /gene="ssuD"
                     /locus_tag="PN977_001887"
     CDS             complement(29931..31076)
                     /gene="ssuD"
                     /locus_tag="PN977_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMNH2-dependent alkanesulfonate monooxygenase"
                     /protein_id="HDI6041918.1"
                     /translation="MSLNMFWFLPTHGDGHYLGTEEGSRPVDHGYLQQIAQAADRLGY
                     TGVLIPTGRSCEDAWLVAASMIPVTQRLKFLVALRPSVTSPTVAARQAATLDRLSNGR
                     ALFNLVTGSDPQELAGDGVFLDHSERYEASAEFTQVWRRLLLGETVDFNGKHIHVRGA
                     KLLFPPIQQPYPPLYFGGSSDVAQELAAEQVDLYLTWGEPPELVKEKIEQVRAKAAAH
                     GRKIRFGIRLHVIVRETNGEAWQAAERLISHLDDETIAKAQAAFARTDSVGQQRMAAL
                     HNGKRDNLEISPNLWAGVGLVRGGAGTALVGDGPTVAARINEYAALGIDSFVLSGYPH
                     LEEAYRVGELLFPHLDVAIPEIPQPQPLNPQGEAVANDFIPRNVAQS"
     gene            complement(31073..32032)
                     /gene="ssuA"
                     /locus_tag="PN977_001888"
     CDS             complement(31073..32032)
                     /gene="ssuA"
                     /locus_tag="PN977_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415456.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter
                     substrate-binding protein SsuA"
                     /protein_id="HDI6041919.1"
                     /translation="MRKLIKLVLAGLLSVSTLAVAAESSPEALRIGYQKGSIGMVLAK
                     SHQLLEKRYPQTKISWVEFPAGPQMLEALNVGSIDLGSTGDIPPIFAQAAGADLVYVG
                     VEPPKPKAEVILVADNSPIKTVADLKGHKVAFQKGSSSHNLLLRALRQAGLKFTDIQP
                     TYLTPADARAAFQQGNVDAWAIWDPYYSAALLQGGVRVLKDGTDLNQTGSFYLAARPY
                     AEKNGAFIQGVLATFSEADALTRSQREQSIALLAKTMGLPAPVIASYLDHRPPTTIKP
                     LSAEVAALQQQTADLFYENRLVPKKVDIRQRIWQPTQLEGKQL"
     gene            complement(32025..32600)
                     /gene="ssuE"
                     /locus_tag="PN977_001889"
     CDS             complement(32025..32600)
                     /gene="ssuE"
                     /locus_tag="PN977_001889"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HDI6041920.1"
                     /translation="MRVITLAGSPRFPSRSSSLLEYAREKLNGLDVEIYHWNLQNFAP
                     EDLLYARFDSPALKTFTEQLQQADGLIVATPVYKAAYSGALKTLLDLLPERALQGKVV
                     LPLATGGTVAHLLAVDYALNPVLSALKAQEILHGVFADDSQVIDYQHKPLFTPNLQTR
                     LDTALETFCQALHRRDVQVPDLLSLRGNAHA"
     gene            32955..33494
                     /locus_tag="PN977_001890"
     CDS             32955..33494
                     /locus_tag="PN977_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HDI6041921.1"
                     /translation="MKKNVLAAFITVICTTSSVMAADDNAITDGSVTFNGKVIAPACT
                     LVAATKDSVVTLPDVSATKLQSNGQVSGVQTDVPIALEDCDITVTKNATFTFNGTADT
                     TQITAFANQASSEAATNVALQMYMNDGTTVITPDTETGNILLQSGNQTLTFKVDYIAT
                     GKATSGNVNAVTNFHINYY"
     gene            33575..34276
                     /locus_tag="PN977_001891"
     CDS             33575..34276
                     /locus_tag="PN977_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HDI6041922.1"
                     /translation="MKTCITKGIVTVSLTAILLSCSFTWAAGKGGVGLAATRLVYSEG
                     EEQISLGVRNTSPDVPYLIQSWVMTPDNKKSADFIITPPLFVLNPANENLLRIMYIGA
                     PLAKDRETLFFTSVRAVPSTTKREEGNTLKIATQSVIKLFWRPKGLAYPLAEAPAKLR
                     CTSSADMVTVSNPTPYFITLTDLKIGGKVVKNQMISPFDKYQFSLPMGAKNSSVTYRT
                     INDYGAETPQLNCKS"
     gene            34301..>36049
                     /locus_tag="PN977_001892"
     CDS             34301..>36049
                     /locus_tag="PN977_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis usher protein"
                     /protein_id="HDI6041923.1"
                     /translation="MYRTQRQHSLLSSGGVPSFIGGLVVFVSAAFNAQAETWFDPAFF
                     KDDPSMVADLSRFEKGQKITPGVYRVDIVLNQTIVDTRNVNFVEITPEKGIAACLTTE
                     SLDAMGVNTDAFPAFKQLDKQACAPLAEIIPDASVTFNVNKLRLEISVPQIAIKSNAR
                     GYVPPERWDEGINALLLGYSFSGANSIHSSAGSDSGDSYFLNLNSGVNLGPWRLRNNS
                     TWSRSSGQTAEWKNLSSYLQRAVIPLKGELTVGDDYTAGDFFDSVSFRGVQLASDDNM
                     LPDSLKGFAPVVRGIAKSNAQVTIKQNGYTIYQTYVSPGAFEISDLYSTSSSGDLLVE
                     IKEADGSVNSYSVPFSSVPLLQRQGRIKYAVTLAKYRTNSNDQQESKFAQATLQWGGP
                     RGTTWYGGGQYAEYYRAAMFGLGFNLGDFGAISFDATQAKSTLADQSEHKGQSYRFLY
                     AKTLNQLGTNFQLMGYRYSTSGFYTLSDTMYKHMDGYEFNDGDDEDTPMWSRYYNLFY
                     TKRGKLQVNISQQLGEYGSFYLSGSQQTYWHTDQQDRLLQFGYNTQIKDLSLGVSWNY
                     SKSRGQPDADQVFALNF"
BASE COUNT         8825 a         9009 c         9597 g         8618 t
ORIGIN      
        1 cgtgcagttc atggtcaact taatgatggc ggctttgata gccagcgtta tgccattgcc
       61 cagcatcaac cgctcaccgg ccgttttctg taggcaagtg tcattgaacc gaattgtagc
      121 ctgcgtgcac agtatctggc gtcattacaa acaacgctgc aaccctatcc gtggaatgcg
      181 gttattcttg gtttaggtat gggggaacgg ttatccgttc ccaaagaaat caaaaatatc
      241 atgcgcgata ctggaacggc gcatttaatg gcgatatcgg gattgcatat cgcttttgcg
      301 gcgttgctgg ctgccggact cattcgcggt gggcaagttt ttctgcctgg gcgctggatc
      361 cactggcaaa tgccattaat tggcggaatc tgctgtgctg ctttttatgc ctggctgact
      421 gggatgcaac ctcctgcatt gcgtaccgtg gtggcgcttg ctacgtgggg aatgcttaag
      481 ttaagtgggc gacaatggag tggctgggat gtatggatat gttgtctggc ggcaattttg
      541 ctgatggatc ctgttgccat tctctcgcaa agtttatggc tctctgccgc tgcggtcgcg
      601 gcactgattt tttggtatca gtggtttccc ggtcctgagt ggcaactgcc gccggtattg
      661 cgtgcacttg tttccctcat ccatctgcaa ctgggaatca cactcctgct tatgcccgtg
      721 caaatcgtaa tatttcatgg cattagtctg acctcgttta ttgcaaatct atttgcaatt
      781 cccctggtga catttatcac ggttccgttg atcctcgccg ctatggttgt gcatttaagc
      841 gggccgttaa tcctggaaga gggattatgg tttcttgccg accggtcttt ggctttactt
      901 ttctgggggt taaagagttt gccggaaggg tggatcaaca ttgctgaacg ttggcaatgg
      961 ctatcatttt ccccatggtt cttactggtg gtatggcgat taaacgcctg gcgaacgttg
     1021 ccagcaatgt gtgtggctgg aggcttgctg atgtgctggc cgctgtggca aaaacctcga
     1081 cctgacgagt ggcaagtgta catgcttgat gtcgggcaag ggctggcaat ggtgatagcc
     1141 agaaacggca aagcgattct ctatgacacg ggactggcct ggcctgaagg ggatagtggg
     1201 caacaactga ttatcccctg gctccactgg cataatcttg aaccggaagg cgttattctg
     1261 agtcatgaac atctggatca ccggggaggg ctggactcaa tattgcatac atggccgatg
     1321 ttatggatca gaagtccgtt aaactgggaa caccatcagc cctgtgtgcg tggcgaagcg
     1381 tggcaatggc aaggattgcg tttcagcgtg cactggcctt tacaagctag caacgataaa
     1441 ggaaataacc attcctgtgt ggttaaggtt gatgacggga cgaatagcat tcttctaacc
     1501 ggtgatattg aagcccccgc tgaacaaaag atgctaagcc gttactggca gcaagtgcag
     1561 gcaacattgc ttcaggtacc tcaccatggc agtaatacct catcatcgtt gccattaatt
     1621 cagcgagtga atggaaaagt ggcactcgca tcggcatcgc gctataacgc atggcgactg
     1681 ccctcaagca aagttaaaca tcgctatcaa caacagggtt ataagtggct tgatactcca
     1741 catcagggtc aaataacggt cgatttttca gcgcaaggct ggcggattag cagcctcaga
     1801 gagcaaattt tacctcgttg gtatcatcag tggtttggcg tgccagtgga taacgggtag
     1861 aatatgcggc tatttcaaca aatgctggtt ttttgaatgc ataacgacaa agatctctct
     1921 acgtggcaga cgttccgccg actgtggcca accattgcgc cttttaaagc gggtctgatc
     1981 gtggcgggcg tagcgttaat cctcaacgca gccagcgata ccttcatgtt atcgctcctt
     2041 aagccacttc ttgatgatgg ctttggtaaa acagatcgct ccgtgctggt gtggatgccg
     2101 ctggtggtga tcgggctgat gattttgcgt ggtatcacca gctatgtctc cagctactgt
     2161 atctcctggg tatcaggaaa ggtggtaatg accatgcgtc gccgcctgtt tggtcacatg
     2221 atgggaatgc cggtttcatt ctttgacaaa cagtcaacgg gtacgctgtt gtcacgtatc
     2281 acctacgatt ccgaacaggt tgcttcttcc tcttccggcg cactgattac tgttgtgcgt
     2341 gaaggtgcgt cgatcatcgg cctgttcatc atgatgttct attacagttg gcaactgtcg
     2401 atcattttga ttgtgctggc accgattgtt tcgattgcga ttcgcgttgt atcgaagcgt
     2461 tttcgcaata tcagtaaaaa catgcagaac accatggggc aggtgaccac cagcgccgaa
     2521 caaatgctga aaggccataa agaagtattg attttcggtg gtcaggaagt ggaaacgaaa
     2581 cgcttcgata aagtcagcaa ccgaatgcgt cttcagggga tgaaaatggt ttcagcctct
     2641 tccatctctg atccgatcat tcagctgatc gcctctttgg cgctggcgtt tgttctgtat
     2701 gcggcaagct tcccaagtgt catggatagc ctgactgccg gtacgattac cgttgttttc
     2761 tcttcaatga ttgcacttat gcgtccgctg aaatcgctga ccaacgttaa cgcccagttc
     2821 cagcgcggta tggcggcttg tcagacgctg tttaccattc tggacagtga gcaggagaaa
     2881 gacgaaggta agcgcgtgat cgagcgtgcg actggcgacg tggaattccg caatgtcacc
     2941 tttacttatc cgggacgtga cgtacccgca ttgcgtaaca tcaacctgaa aattccggca
     3001 gggaagacgg ttgctctagt tggacgctct ggttcaggta aatcaaccat cgccagcctg
     3061 atcacgcgtt tttacgatat tgatgaaggc gaaatcctga tggatggtca cgatctgcgc
     3121 gagtataccc tggcgtcgtt acgtaaccag gttgctctgg tgtcgcagaa tgtccatctg
     3181 tttaacgata cggttgccaa caacattgct tacgcacgga ctgaacagta cagccgtgag
     3241 caaattgaag aagcggcgcg tatggcctac gccatggact tcatcaataa gatggataac
     3301 ggtctcgata cagtgattgg tgaaaacggc gtgctgctct ctggcggtca gcgtcagcgt
     3361 attgctatcg ctcgagcctt gttgcgtgat agcccgattc tgattctgga cgaagctacc
     3421 tcggcgttgg ataccgaatc cgaacgtgcg attcaggcgg cactggatga gttgcagaaa
     3481 aaccgtacct ctctggtgat tgcccaccgc ttgtctacca ttgaaaaggc agacgaaatc
     3541 gtggtcgtcg aggatggtgt cattgtggaa cgcggtacgc ataacgattt gcttgagcac
     3601 cgtggcgttt acgcgcaact tcacaaaatg cagtttggcc aatgatcgaa aaaatctggt
     3661 ctggtgaatc ccctttgtgg cggctattgc tgccactctc ctggttgtat ggcctggtga
     3721 gtggcgcgat ccgtctttgc tataaactaa aactgaagcg cgcctggcgt gcccccgtac
     3781 cggttgtcgt ggttggtaat ctcaccgcag gcggcaacgg aaaaaccccg gtcgttgtct
     3841 ggctggtgga acagttgcaa cagcgcggta tttgcgtggg ggtcgtatcg cggggatatg
     3901 gtggtaaggc tgaatcttat ccgctgttat tgtcggcaga taccaccaca gcacaggcgg
     3961 gtgatgaacc tgtgttgatt tatcaacgca ctgatgcgcc tgttgcggtt tctcccgtgc
     4021 gttctgatgc gataaaagcc attctggcgc aacaccctga tgtgcagatc atcgtaaccg
     4081 acgacggttt acagcattac cgtctggcgc gtgatgtgga aattgtcgtt attgatggtg
     4141 tgcgtcgctt tggcaatggc tggtggttgc cggcggggcc aatgcgtgag cgagcggggc
     4201 gcttaaagtc ggttgatgcg gtaatcgtca acggtggtgt cccccgcagc ggtgaaatcc
     4261 ccatgcatct gctgccgggt caggcggtga atttacgtac cggtacgcgt tgtgacgttg
     4321 ctcagcttga acatgtggtg gcgatggcag ggattggtca tccgccgcgc ttttttgcca
     4381 cgctgaagat gtgtggcgta caaccggaaa aatgtgtacc gctggccgat catcagtctt
     4441 tgaatcatgc ggatgtcagc gcgttggtca gcgccgggca aacgctggta atgacagaaa
     4501 aagatgcggt gaaatgccgg gcctttgcag aagaaaattg gtggtatttg cctgtagacg
     4561 cacagctttc aggtgatgaa ccagcgaaac tgcttgcgca actaacctcg ctggcttctg
     4621 gcaactagtt acgccgcggc agcgttcgat tgatggagtc atgaatgtcg ctgccgcacc
     4681 tctctcttgc tgatgcgcgt aatcttcacc tcgccgcgca aggcctgtta aacaaacccc
     4741 gccgtcgagc gtcgttggag gatattccgg caacgatctc ccgcatgtcc ttgctgcaaa
     4801 tcgataccat caatattgtt gcccgtagcc catatctggt gcttttcagt cgtctgggaa
     4861 attatcctgc ccagtggctg gatgagtctc tggcgcgtgg cgaattaatg gaatactggg
     4921 cgcatgaagc ctgctttatg ccacgtagcg attttcgtct tattcgccac cgcatgctgg
     4981 cacctgaaaa aatgggctgg aaatacaaag acgcctggat gcaggaacat gaggtggaaa
     5041 ttgcacagtt aattcagcat attcatgata aggggccggt acgttcagcc gattttgagc
     5101 atcctcgtaa aggggcaagc ggctggtggg agtggaagcc gcataaacga catctggaag
     5161 gtttatttac tgccggaaag gtgatggtga ttgaacggcg caacttccag cgcgtttatg
     5221 atttaaccca ccgtgtcatg cctgactggg atgatgagcg cgatctcgtt tcgcaaacag
     5281 aagcagaaat catcatgctg gataacagtg cgcgtagcct gggcatattc cgcgaacagt
     5341 ggctggcaga ttactatcgg ctgaaacgtc cggcactggc ggcttggcgc gaagcgaggg
     5401 ctgaactgca gcaaatcatt gctgtgcatg ttgaaaaatt gggcaatctt tggttgcatg
     5461 ctgatttgct gccgctactc gagcgtgcgc tggccggaaa gctcactgca acgcacagcg
     5521 cggtactttc gccttttgat cctgttgtct gggatcgcaa acgcgcagag cagctttttg
     5581 attttagcta ccggctggag tgctataccc ctgcgccgaa acgccagtat ggctattttg
     5641 ttctgccgtt attacatcgt gggcaattag ttgggcgaat ggatgccaaa atgcatcgcc
     5701 agacaggcat ccttgaagtt atctctctgt ggttacagga aggcattaaa ccaacgacaa
     5761 cgctgcaaaa agggttacgt caggcgatta ctgatttcgc taactggcag caggcaacgc
     5821 gggtgacatt aggacgctgc ccgcaaggcc tctttactga ttgccgcacc ggctgggaaa
     5881 tagaccccgt cgcataaatg attatgataa gatttatgca ttcataaccc gatccatctg
     5941 gaggaactat ggatcatcgt ctgcttgaaa tcattgcctg cccggtttgc aacggaaaac
     6001 tttggtataa ccaggaaaaa caagaactta tttgcaaact ggacaacctg gctttcccgc
     6061 tgcgcgatgg cattccagtg ttgctggaaa cggaagcccg cgtgctgact gctgatgaga
     6121 gtaaatcatg agttttgtgg tcattattcc cgcgcgctac gcgtccacgc gtctgcccgg
     6181 taaaccattg gttgatatta acggtaaacc catgattgtt catgttcttg aacgcgcgcg
     6241 cgaatcaggt gccgagcgca tcatcgtggc aaccgatcat gaggatgttg cccgcgctgt
     6301 tgaagccgct ggcggtgaag tatgtatgac gcgcgccgat catcagtcag gaacagaacg
     6361 tctggcggaa gttgtcgaaa aatgcgcatt cagcgacgac acggtgatcg ttaatgtgca
     6421 gggggatgaa ccgatgatcc ctgcgacaat cattcgtcag gttgctgata atctcgccca
     6481 gcgtcaggtg ggtatggcga ctctggcggt gccaatccac aatgcggagg aagcgtttaa
     6541 cccgaatgcg gtgaaagtgg tcctcgacgc tgaagggtat gcactgtact tctctcgcgc
     6601 caccattcct tgggatcgtg atcgttttgc aaaaggcctt gaaaccgttg gcgataactt
     6661 cctgcgtcat cttggtattt atggctaccg tgcaggattt atccgtcgtt acgtcaactg
     6721 gcagccaagt ccgttagaac acatcgaaat gttagagcag cttcgtgttc tgtggtacgg
     6781 cgaaaaaatc catgttgctg tcgcccagga agttcctggc acaggtgtgg atacccctga
     6841 agatctcgag cgcgttcgcg ctgaaatgcg ctaatttcac ttcacgacac tccagccaat
     6901 tttgggagga gtgtcgtacc gttacgattt tcctcaattt ttcttttcaa caattgatct
     6961 cattcaggtg acatctttta tattggcgct cattatgaaa gcagtagctt ttatgagggt
     7021 aatctgaatg gaacagctgc gtgccgaatt aagccattta ctgggcgaaa aactcagtcg
     7081 tattgagtgc gtcaatgaaa aagcggatac ggcgttgtgg gctttgtatg acagccaggg
     7141 aaacccaatg ccgttaatgg caagaagctt tagtacgccc ggaaaagccc gacaactggc
     7201 atggaaaacc accatgctgg caagaagtgg gactgtccgt atgccgacta tttatggtgt
     7261 gatgacgcat gaagaacacc ccggccccga tgtcctgcta ctggagcgga tgcgtggtgt
     7321 ttcggtggag gcaccagccc gaacaccaga acgctgggaa caactcaaag atcaaatcgt
     7381 tgaagcctta ctggcctggc accgtcagga cagtcgcggt tgcgtcggcg cggtcgacaa
     7441 tactcaggaa aatttctggc cctcatggta ccggcaacat gttgaagtgc tatggaccac
     7501 gctcaatcag ttcaataaca ccggtctgac gatgcaggat aagcggatcc tgtttcgcac
     7561 tcgcgaatgt ctcccggcat tatttgaagg ctttaacgac aattgtgtac tgattcacgg
     7621 taacttctgt ttacgcagca tgctgaaaga ttcgcgcagc gatcagttac tggcgatggt
     7681 cggaccggga ctaatgcttt gggcaccgcg agaatacgaa ctgttccgac taatggataa
     7741 ttctctggcg gaagatttgc tctggagtta tctgcaacgc gcgccagtgg cggagtcgtt
     7801 catctggcgg cgttggttgt atgtgttatg ggatgaagtt gcgcaactgg tcaacaccgg
     7861 acgatttagt cggcgtaact tcgatctggc atcaaaatca ctcttgccgt ggctcgcctg
     7921 acgaaccttt cagccattgc cagatccggc caagcgtttc atagccaacg cggtcgctat
     7981 gcatcaacca aaccggagaa gggattgccc gttcccacgg attaagcggc gagtcgatgg
     8041 ccaactgatt cgccggggct ggtagcggat ttaacccttc ctgctgaaaa aagatcatcg
     8101 cccgcggcag atgtgatgcg gaggtcacca gcaggaaagg ggcgtcacca atcgcctgtt
     8161 tcactgccgc agcttcttct tcggtatctt ttggcaaatc cagggtgata atttgctcgc
     8221 gcggtacgcc cagcgattgc gcaactctgg cacctacttc cgctgtactc accgtattgg
     8281 tttttgctac gcctcccgtg aagatcagtt ttgatcccgg attttcgcgc cataagcgaa
     8341 taccttcatt caggcgtggc agactgttat tgattaaatt cgaactcggt gcccactgtg
     8401 ggttccaggt ataaccgcct ccgagcacca cgatatagtc cactttttgc gaattattcc
     8461 atgtcggata ggtgctttcg atgggacgca gcaggcggtc ggctaccggt tgcaaactca
     8521 ataacaagag cgccagccac ccaatgctaa taaaaatctt cccggttttt tgaaaacggc
     8581 taaaccacag cagcgccagg ccagcgccaa taatcaacaa catcagcgga agtggcagca
     8641 acatattgcc aatcactttt ttcagtgtaa aaagcatcct ttctggttcc ttttttaacc
     8701 atatagcaag ggatcgccag cgatattaca ccagacgggt tcattctcgc cgtggctgtg
     8761 acaaaatagc gtctttatca gaaaacgtct tagccaaccg gtggagaaag caatgcagga
     8821 tcgcaatttt gatgatattg cggaaaagtt ttcccgtaac atttacggca ccaccaaagg
     8881 gcagcttcga caggctattc tgtggcagga tctcgatcgc gtgctggcgg aaatgggccc
     8941 gcaaaaactg cgtgtgctgg atgctggcgg tggagaaggg cagaccgcaa tcaaaatggc
     9001 tgagcgcggg catcaggtca ttttatgcga tctttctgcg cagatgatcg accgcgcaaa
     9061 acaggcggca gaagcaaaag gtgtgagcga caacatgcaa tttatacatt gcgccgctca
     9121 ggatgttgct tcgcatttgg aaacgcccgt tgatctgata ttgttccatg cggtgctcga
     9181 gtgggtggcc gatccccgca gcgtattaca gaccctgtgg tcagtgttgc gtccaggcgg
     9241 cgtgttgtcg ttaatgttct acaatgcgca cggcttgctg atgcataaca tggtcgccgg
     9301 gaattttgat tacgtgcagg cgggaatgcc gaaaaagaaa aaacggacgc tttcgccaga
     9361 ttatccacgc gacccggcgc aggtttatct gtggctggaa gaagctggtt ggcaaattat
     9421 gggtaagaca ggcgttcgtg tgtttcatga ttatctgcgc gagaagcacc agcagcgcga
     9481 ctgctatgaa gcattacttg aattagaaac gcgttattgc cgtcaggaac cgtatattac
     9541 cctggggcgt tatattcatg tcaccgcgcg caaaccgcag agcaaggata aagtatgagt
     9601 gaattttccc agacagtccc cgaactggtt gcctgggcca gaaaaaatga cttctccatc
     9661 tcgctgccgg tagaccgact ctcttttctg ctggcggttg ccacgctgaa cggcgagcgt
     9721 ctggatggtg agatgagtga aggcgagctg gtggatgcat tccgccatgt gagtgatgcg
     9781 tttgagcaaa ccagcgaaac catcggcgtg cgtgccaaca acgcgatcaa cgacatggtg
     9841 cgtcaacgtc tgctgaaccg ctttaccagc gagcaggcgg aagggaacgc aatttaccgt
     9901 ctgacgccgc tcggcatcgg cattactgac tattacatcc gtcagcgcga gttttctacg
     9961 ctgcgccttt ctatgcagtt gtcgattgtg gcgggtgagc tcaaacgcgc ggcagatgcc
    10021 gccgaagagg gcggtgatga atttcactgg caccgtaatg tttatgcgcc actgaaatat
    10081 tcggtagcag aaattttcga cagtatcgac ctgacgcaac gtctgatgga cgaacagcag
    10141 cagcaggtga aggacgatat cgcccagttg ctgaacaaag actggcgggc ggcgatctcc
    10201 agctgtgaat tgttgctttc ggaaacttcc ggaacgctgc gtgaattgca ggatacgctg
    10261 gaagcggcag gcgacaaatt gcaggctaat ctgttgcgca ttcaggatgc gacgatgacc
    10321 catgacgatc tgcattttgt cgatcgtctg gtgttcgatc tgcagagcaa acttgaccgt
    10381 attatcagtt ggggccagca atccatcgac ttgtggattg gctacgaccg ccacgtacac
    10441 aaatttatcc gtaccgcaat cgatatggat aaaaaccgcg tctttgctca gcggttacgt
    10501 cagtcggtac aaacctattt tgatgagccg tgggcgctaa cttatgccaa tgccgatcgt
    10561 ctgctggata tgcgtgacga agagatggca ctgcgcgatg aagaagtgac tggggaactt
    10621 cctgaggatc tggagtacga agagtttaac gagatccgcg aacagctggc ggcgatcatt
    10681 gaagaacaac ttgccgtgta caaaaccaga caagtgccgc tggatcttgg tctggtggta
    10741 cgcgaatatc tgtcacagta tccgcgtgca cgtcactttg acgttgcgcg cattgttatt
    10801 gatcaggcgg tacgtcttgg cgtagcgcaa gcagatttca ccggactgcc agcgaaatgg
    10861 cagccgatta atgattacgg agccaaggta caggcgcatg tcatcgacaa atattgaaca
    10921 agtgatgccg gttaagctgg cgcaggcgct ggcgaatccg ttatttccgg cgctggatag
    10981 cgccttacgt tcaggccgcc atattggcct cgacgaactg gataatcatg cattcctgat
    11041 ggattttcag gaatatctgg aagagtttta cgcgcgttat aacgttgagc ttattcgcgc
    11101 accggaaggg ttcttctatt tacgcccacg ttccaccact cttatcccgc gttccgtctt
    11161 gtcggaactg gatatgatgg tcgggaaaat cctctgttat ctctatctca gcccggaacg
    11221 gctggcgaac gaggggattt tcacccagca ggaactgtac gacgaactgc tcaccctggc
    11281 cgatgaagcg aaactgctga aactggtgaa caaccgttca accggttcag acgttgaccg
    11341 ccagaagttg caggagaaag tacgttcttc gctcaaccgt ctgcgtcgtt tgggtatggt
    11401 gtggtttatg ggccacgaca gcagcaagtt ccgcattacc gaatcggtgt tccgcttcgg
    11461 agccgatgtg cgtgctggcg acgatccccg tgaagcacag cgtcgcctga ttcgtgatgg
    11521 cgaagcaatg ccgattgaaa atcatctgca actcaacgat gaaaccgaag agaatcagcc
    11581 agatagcgga gaggaagaat aatgattgaa cgcggtaaat ttcgctcact gacgctgatt
    11641 aactggaacg gcttttttgc ccgaactttt gaccttgacg agctggtcac gacgctttct
    11701 ggcggtaacg gggcgggtaa atccaccacc atggcggcgt tcgttacggc gctgatcccc
    11761 gacctgaccc tgctgcattt ccgtaacacc acggaagccg gggccaccag cggttcgcgc
    11821 gataaaggtc tgcacggtaa gctgaaagcg ggtgtctgtt attcgatgct cgacaccatt
    11881 aactcgcgcc accagcgcgt ggtggtcggt gtgcgtctgc aacaggttgc cggacgcgat
    11941 cgtaaagtgg atatcaaacc gtttgccatt cagggtttac cgatgtcggt gcagccgaca
    12001 caactggtga ccgaaaccct gagcgaacgt caggcgcgcg tgctgccgct taacgagctg
    12061 aaagacaagc tcgaggcgat ggaaggcgtg cagtttaaac agttcaactc cattaccgat
    12121 taccactcgc tgatgttcga tctgggcatc atcgcgcgtc gtctgcgctc agcgtctgac
    12181 cgtagtaaat tctatcgtct gatcgaagct tcgctgtacg gcgggatctc cagcgccatt
    12241 acgcgctctc tgcgcgacta cctgttgccg gaaaacagcg gcgtgcggaa agcgttccag
    12301 gacatggaag cggcgctgcg cgaaaaccgc atgacgctgg aagcgattcg tgtcacccag
    12361 tcggaccgcg acctgtttaa gcatctgatt agcgaagcca ccaactacgt ggcggcggac
    12421 tacatgcgtc acgccaacga gcgccgtgtc catctcgaca aagccctgga gtttcgtcgc
    12481 gagctacaca cttcgcgtca gcaactggcg gctgagcagt acaaacacgt cgatatggcg
    12541 cgtgagctgg cagagcacaa cggtgccgaa ggcgatctgg aagcagatta tcaggcggcc
    12601 agcgatcacc tgaacctggt gcaaaccgca ctgcgtcagc aggagaagat cgaacgctac
    12661 gaagcggatc tcgatgagct gcagatccgt ctggaagagc aaaacgaagt ggtggcagaa
    12721 gccatcgaac gccaggaaga gaacgaggct cgtgcggaag ctgccgaact ggaagtggac
    12781 gagctgaaaa gccagcttgc tgactaccag caggcgctgg acgtccagca aacgcgcgcg
    12841 atccagtata accaggcgat tgctgcgctt aatcgtgcca aagaactgtg ccatctgccg
    12901 gacttaaccg ccgacagcgc cgccgaatgg ctggaaacct tccaggcgaa agagctggaa
    12961 gcgaccgaaa aaatgctctc tcttgagcag aaaatgagca tggcgcaaac cgcgcacagc
    13021 cagtttgagc aggcttatca gctggtggtg gcaatcaacg gcccgctggc gcgtaacgag
    13081 gcgtgggatg tcgctcgcga actattgcgc gaaggggtcg atcagcgtca cctggcagag
    13141 caggttcagc cgttgcggat gcgcttaagc gaactggaac agcgtctgcg tgagcagcaa
    13201 gaagctgagc gtctgctggc agatttctgc aaacgtcagg gcaagaattt tgacattgac
    13261 gagctggaag ctctgcatca ggagctggaa gcacgcattg cctctctttc cgatagcgtg
    13321 tctaacgccc gtgaagagcg gatgacactg cgccaggagc aggaacagct gcagtctcgc
    13381 attcagagtt tgatgcggcg tgcgccggtt tggctggctg cgcaaaacag tctcaaccag
    13441 ttgagcgaac agtgcggcga agagttctcc tccagccagg atgtcaccga atatctgcaa
    13501 cagttgctgg agcgtgagcg cgaagccatt gttgaacgcg atgaagtagg cgcgcgcaaa
    13561 aacgccgtcg atgaagagat cgaacgttta agccagccag gtggttctga agaccagcgt
    13621 ctgaatgcgc tggcggagcg ttttggcggc gtgctgctgt cagaaattta tgacgacgtt
    13681 agcctggaag atgcgccgta cttctcagcg ctgtatggcc cgtcacgcca cgccatcgtg
    13741 gtgccagatc tgtcacaggt aaccgaacac ctggaaggct tgaccgattg cccggaagat
    13801 ctctatctga tcgaagggga tccgcagtca ttcgatgaca gcgtgttcag cgttgatgag
    13861 ctggaaaaag cggtggtggt gaaaatcgcc gatcgtcagt ggcgttattc acgtttcccg
    13921 gaagtgccgc tgtttggtcg tgctgcgcgt gaaagccgta ttgaaagcct ccatgccgag
    13981 cgtgaagtgc tttccgaacg cttcgccacg ctctcctttg atgtacagaa aacccagcgt
    14041 ctgcatcagg cgttcagccg ctttatcggc agccacctgg cggtggcgtt tgagtctgat
    14101 ccggaagccg aaatccgtca actgaacagc cgtcgcgtcg aactggagcg ggcgttgagt
    14161 aatcatgaaa atgataacca gcagcagcgt attcagtttg agcaggcgaa agagggtgtt
    14221 acggcgctga accgcattct gccgcgtctc aacttgttgg ctgatgacag cctggcggat
    14281 cgcgtcgatg aaatccgcga acgtctggat gaagcccagg aagccgcgcg ttttgttcag
    14341 cagttcggta atcaactggc gaaactggaa ccgatcgttt cggtattgca gagtgacccg
    14401 gaacagttcg aacaattaaa agaagattac gcgtactctc agcagatgca gcgcgatgcc
    14461 cgtcagcagg cgtttgccct gacggaagtg gtgcagcgtc gtgcgcactt tagctactct
    14521 gactcggcag aaatgcttag cggtaacagc gatctcaacg aaaaactgcg tgaacgtctg
    14581 gaacaggcgg aagcggagcg tacccgcgcg cgtgaagcgt tgcgcggaca cgcagcgcag
    14641 ttgagtcagt acaaccaggt gctggcttcg ctgaaaagtt cttacgacac caaaaaagag
    14701 ctactcaacg atctgcaacg tgaattgcag gatatcggcg tgcgtgctga tagcggggca
    14761 gaagagcggg cgcgtattcg ccgtgacgag ttgcacgcgc aactgagcaa taaccgttca
    14821 cgccgcaatc aactggaaaa agcgcttacc ttctgcgaag cagagatgga caacctgacc
    14881 cgcaaactgc gcaagctgga gcgggattac tttgagatgc gcgagcaggt agtgaccgcg
    14941 aaagcgggct ggtgtgcagt catgcgcatg gtgaaagata acggtgttga gcgccgctta
    15001 caccgtcgtg agctggctta tctctccgct gatgatttgc gttccatgtc ggataaggcg
    15061 ttaggtgcgc tgcgtctggc ggtggcggat aacgaacatc tgcgcgacgt gctgcggatg
    15121 tcggaagatc cgaaacgtcc ggagcgtaaa attcagttct tcgtggcggt ttatcagcat
    15181 ctgcgcgagc gtattcgtca ggatattatt cgtaccgatg atccggttga agctatcgag
    15241 cagatggaga tcgaacttag tcgtctgact gaagagttaa cgtcccgcga acagaaactg
    15301 gcgatcagtt cccgcagcgt ggcgaacatc atccgtaaaa ccattcagcg cgagcagaac
    15361 cgtatccgta tgctcaacca gggcttgcag aacgtatcgt tcggtcaggt gaacagcgtg
    15421 cgcctcaacg tgaacgtgcg tgaaacgcat gccatgctgc tggatgtgct ctctgaacag
    15481 cacgagcaac atcaggatct gtttaacagc aaccgtctga ccttctcgga agcgctggcg
    15541 aaactgtatc aacgtcttaa cccgcagatt gatatggggc agcgcacgcc gcagaccatc
    15601 ggtgaagaac tgctggatta ccgcaactat ctggaaatgg aagttgaggt taaccgtggt
    15661 tccgatggct ggctgcgcgc agaatctggc gctctgtcga ccggtgaggc gattggtacc
    15721 ggtatgtcga ttctggtgat ggtggtacaa agctgggaag atgaatctcg ccgcctgcgc
    15781 ggtaaagata tctctccttg ccgcctgctg ttcctcgatg aagcagcgcg actggatgct
    15841 cgttccatcg ccacgctgtt tgaattgtgt gagcgtttgc aaatgcagct catcatcgca
    15901 gcgccggaaa atatcagccc ggaaaaaggc accacctata aactggtgcg taaagtcttc
    15961 cagaataccg aacacgttca cgtagtcggt ctgcgtggtt ttgcaccgca actccctgaa
    16021 acgcttccag gaagtgacga agcgccttcc caggcgagct aagattgagg cggcagcgat
    16081 gccgcctttt ctatttctga aaactccgtt tctgcactaa aaaaatgcca cattctggcg
    16141 cgcttttctt taaacttctt tacattaggt tatgtaaaaa cgtatcggcg tttatatact
    16201 gaagataagc ctgatgagta acaggcttgc tcgtcatact ttcgtgagta ttggcgttgt
    16261 acaggcaagt cgtaaaataa cagcctggct attcagagta tgataaaaac agggggcaag
    16321 ggatgttgct taatatgatg tgtggtcgtc ggctgtcggc aatcagtttg tgcgtggccg
    16381 taacattcgc tccactgttc aatgcgcagg ccgatgagcc tgaagtaatc cctggcgaca
    16441 gcccggtggc tgtcagtgaa cagggcgagg cactgccgca ggcgcaagcc acggcaatta
    16501 tggcggggat ccagccattg cctgaaggtg cggcagaaaa agcccgcacg caaatcgaat
    16561 ctcaattacc cgcaggttat aagccggttt atcttaacca gcttcaactg ttgtatgccg
    16621 cacgcgatat gcaacccatg tgggaaaacc gtgatgctgt taaagccttc cagcaacagc
    16681 tggcagaggt ggcgattgcc ggtttccagc cgcagtttaa taaatgggta gagttactga
    16741 ccgatcctgg tgttaacggg atggcacgcg acgtcgtgct ctctgatgcg atgatgggct
    16801 atctccattt cattgcaaat attccggtca aaggcactcg ctggctatat agcagtaaac
    16861 cttatgcgct ttcaacgccg ccgctttcgg tgattaacca atggcagctg gcgctggata
    16921 aaggtcaatt gccgacgttt gttgcaggtc tggcaccgca gcatccgcaa tatgcagcga
    16981 tgcatgaatc gttactggcc ttactcagtg acaccaaacc gtggccccaa ctgaccggca
    17041 aagcaacgtt gcgcccaggg cagtggagta acgacgtacc ggcgttgcgc gaaatattgc
    17101 aacgcacagg catgctggac ggggggccga aaattactct acctggcgat gacacgccaa
    17161 ctgacgcggt agtcagccca tccgctgtta ctgttgaaac agcagaaact aagccgatgg
    17221 acaagcaaac gacgtctcgt agtaaacctg cgcctgccgt tcgtgccgcc tacgataatg
    17281 aactggtgga agccgttaaa cgttttcagg catggcaagg attgggagca gatggcgcta
    17341 ttggcccggc aacgcgtgac tggttaaacg taacgcccgc ccagcgtgct ggcgtgttgg
    17401 cactcaacat ccagcgattg cgcttgctgc caacagagct ttctaccggg atcatggtta
    17461 acattccggc ctattcgctg gtctactatc agaacggcaa tcaggtgctg gattcgcgag
    17521 tcattgtcgg tcgccccgat cgcaaaacgc cgatgatgag cagtgcactt aacaacgtag
    17581 tggtaaaccc gccgtggaac gtaccaccaa ctctggcacg caaagatatt ctgccgaaag
    17641 tgcgcaacga tccgggatat ctcgaaagcc atggctatac ggtgatgcgc ggctggaaca
    17701 gcagagaagc gattgaccca tggcaggttg actggtctac aatcacggcc tcgaatttac
    17761 cgttccgctt ccagcaggct ccaggcccac ggaactcgct ggggcgctat aaattcaata
    17821 tgccgagttc agaggccatt tatttgcatg acacgccgaa ccacaatctg ttcaaacgtg
    17881 atacacgcgc attgagctca ggctgtgtgc gagtgaataa agcttccgat ctggcgaata
    17941 tgctgttgca ggatgcagga tggaatgaca aacgtatttc tgatgcgctg aagcagggcg
    18001 atacgcgtta cgtcaatatt cggcagtcga ttccggtgaa tctctactac ctgacggcct
    18061 ttgttggtgc agatggtcgt acccagtatc gtacagatat ttacaattat gatctgcctg
    18121 cgcgatccag ctcgcaaatc gtatcgaaag cggaacaatt aatcaggtaa atgaagtagt
    18181 tcggggaaat cagttgtcgt aataattagc atgatcgggg gcgattctct gcagcccccg
    18241 tcactgctgg ggttgagtca tcttgacgtc agctttactg acggttaagg tgcctcttgt
    18301 gcgccagaag tgcatataaa cgataacatt gacctgtaga cttgattatc atggacaaat
    18361 tcgacgctaa tcgccgcaaa ctgctggcgc ttggtggcgt tgcactcggt gccgccatcc
    18421 tgccgacccc ggcgtttgca acactctcta ccccacgccc gcgcattttg acactcaata
    18481 atcttcatac cggagagtca atcaaagcgg agtttttcga tggcagaggc tatattcagg
    18541 aagaattggc aaaacttaac cattttttcc gcgattaccg cgcgaacaaa ataaagtcca
    18601 tcgacccagg attgttcgat cagttgtatc gcctgcaagg gttattaggt acccgcaaac
    18661 cggtgcaact catttccggt tatcgttcca ttgataccaa caatgaacta cgcgcccgca
    18721 gccgtggagt agcgaagaaa agctatcaca cgaaagggca ggcgatggat ttccatattg
    18781 aaggtatcgc gttaagcaat attcgcaaag ccgcgttatc tatgcgcgca ggtggtgtag
    18841 gatattaccc acgtagtaac tttgtgcata ttgataccgg gccagcacgg cactggtagt
    18901 aatcgcttaa cgaaacaggg gcagtatgaa ctatcgtatt attccggtca ccgcattctc
    18961 ccagaactgt tcattgatct ggtgtgaaca aacccgactg gccgcactgg tcgatcctgg
    19021 cggcgatgcg gaaaaaatca aacaggaagt tgatgccagc ggcctgacac tgatgcagat
    19081 cctgctgact catggtcatc tggaccacgt tggcgcagcg gcggaactgg cgcaacatta
    19141 cggcgtgccg gtttttggcc cggaaaaaga agatgagttc tggctgcaag gcttgcctgc
    19201 gcaaagtcgt atgtttggtc tggaagagtg ccagccgctg acgccagatc gttggctgaa
    19261 cgaaggcgat accatcagca tagggaatgt gactttacag gtgttacatt gccctgggca
    19321 tacgccgggt catgtcgtgt tttttgatga gcgggcaagg ctgctgattt ctggcgacgt
    19381 tattttcaaa ggcggagtag ggcgcagtga ctttccgcgt ggcgatcata atcaactgat
    19441 ttcttcaatc aaagataaat tgctgcccct gggggatgac gtgacattta ttccgggtca
    19501 cggaccatta tcaactcttg gttacgaacg cctgcataat cccttcctgc aagacgaaat
    19561 gcccgtctgg taaagacgta taaaaaagcc cgctttcaca gcgggcttcg tttaactagc
    19621 ctttacagca cggccacaat cgcttcgcac agcggagcca tgttatctgg cgtcatcccg
    19681 gccacgttca cacgaccaga agccactgca tacacgccaa actcttcacg cagacgcagt
    19741 acctgttctt tcgtcaggcc actgaaggag aacataccgt tctgtttgat gataaagctg
    19801 aagtcgcggt ttgccccttt ttcctgcagc gtattgacga acaactgacg catacgctga
    19861 atacgctggc gcatatcagt cagctcttgt tcccaaatcg cacgtaacgc atcgttgctc
    19921 aggatggtgg caacaacaga agcgccgtgt gctggtgggt tagagtagtt agcgcgaatc
    19981 gccgctttca tttggctgaa tgcgcgatca acggtctcac tgtcagcagc aaccagagta
    20041 caagcgccaa cacgctcgtt gtacaggcca aagtttttag agtaggaact ggcaacaatc
    20101 agctctttat gcatagcagc gaaagcgcgc agtccttcag catcttcttc cagaccacgg
    20161 gcaaaaccct ggtaagcgaa gtcaaacagc ggtaaccagc ctttctcaac ggaaagttgt
    20221 gccagtgtct gccattgttc cagcgtaggg tcgataccgg ttgggttatg gcagcagcca
    20281 tggaacagca ctacgtcgcc agcctgagct tcgttcaggc tgttaatcag tgcatcgaag
    20341 tcaagggtgt gattttccgc atcataataa gcgtattcac gaacttccag acctgcagag
    20401 ttaaagacgc tcttatggtt cggccagctt gggttgctca cccacacacg cttaacgctg
    20461 gtattttttg ccaggaaatc ggcagccacg cgtagtgcgc cagtgccacc cggagtctgt
    20521 gccgtgcgag cacgtttgtc attgatcagg gcgctacctt taccaaacag cagttcctga
    20581 gtgcagcgac caaattcagg gatgccgtca atgccgaggt aatttttggt ggtttcattt
    20641 tcgagcaaat actgttcagc ctttttcacg ctggtcagta ccggggtttt gcccgtctca
    20701 tctttataga caccaatccc gaggttaatt ttgccgggac gttcatcggc acgaaacaga
    20761 tcggccaggc ccagaatcgg gtcggcagga gcggcggtaa tgttctcaaa catgacgagg
    20821 ttccattatg gttacagaag ggaagtccgc tatcagggta acgggagatt tacaaaattc
    20881 caactattac tgatgaaaac gcaggctgtt tttgcaagac gtgagattgc tctggaaggc
    20941 ataaaaaaaa caggaccaac gtcctgtttt ttcggcattt aacaaagagg tgtgctatta
    21001 gaactggtaa acgataccca cagcaacggt gtcgtctgaa cctacgccca gtttgttgtc
    21061 agaatcgatc tggttgatga tgtagtcaac ataggtggac atgtttttgt tgaagtagta
    21121 ggttgcgccc acttcaaagt agttcaccag atcaacatca ccgatacctt ctacgtcttt
    21181 cgctttagat ttggtgtaag cgatggacgg acgcagaccg aaatcgaact ggtattgcgc
    21241 aactaacaga acgtcttgcg ttttgttggc gaagccgcct tcaattggcg tagcgttacg
    21301 ggtttcaccg tagttcgctg ccaggtagat gttgttcgcg tcatacttca gaccagttgc
    21361 ccactgttca gcttttttac cctgaccgcg gaattcggct tcctgggcgt cggtacggtc
    21421 tgctgcaccg taagcaccta cgataccaaa gccttcgaat tcgtagctga tagagccacc
    21481 gacgccgtcg ccgttggaac gctcaggaat accagcacgt tcgtttttgc ccaggtactg
    21541 aacggcaaag ttcaggccgt caaccagacc gaagaagttg gagttacgat aggtagcaac
    21601 gccgcctaca cgaccaacga agaagtcatc gctgtatgcc gtgtcaccgc caaactcagg
    21661 cagcatatcg gtgtaaccca gtgcgtcgta aaccacaccg tagttacgac cgtagtcgat
    21721 agaacccgca tcagcgaatt tcagacctgc gaatgccaga cgagttttgt tacctttctg
    21781 ggcatcagcg ccttcagagt tgttaccctg gaagttatat tcccactgac cgtagccggt
    21841 cagatcggaa ttgatttgtg tttccccttt aaaaccaaga cgggcataag ttttgtcgcc
    21901 gtttccgccg taactgttta caccattgtc tttagaaaaa taatgcagac cgacagcttt
    21961 accgtacaga tctactttgt tgccatcttt gttatagatt tctgcagcgt ttgcagtacc
    22021 tgctactaac agagcaggga cgatcactgc cagaatattg cgcttcatca ttatttatta
    22081 ccctcatggt ttttttatga cacctgccac tgccgtcaat aagttctgtc aataaaaatt
    22141 tacggaacta ttgatgagag tttggtgtct ttatgtgtct gcaggcatct ttccattcaa
    22201 actaacgttt cgctaccgtg aaagtgctac aaagataaag atttggtttc aaaaagaaaa
    22261 aatatgtaac caaaagtaaa atttaaggaa ctttgtgaac accgtcatat ttccatagaa
    22321 gcgtgatgat atttacagca atttttatct atttatatga tttccttata tttaaattaa
    22381 ctaaaaggaa attttgtttc tgatggaaac tttatcgacc tggcactaaa tcctcgttca
    22441 gacatccaga acgccaaaaa atagtatgaa aattgtgcta tcagctaaga aaaaactaat
    22501 ccgcaataag aatgcggatt attttttgtg cgcaaatgtt gcagtgtaat atttcgtaac
    22561 aggatgatcg ttatggcggg gttgcaaata gattgcttga cttaacaaac agataacttg
    22621 acagaaagga taaaaaaaga gccagcgggc gctggctctt gaagacgcta aattagaagc
    22681 tggcgttacg cggagtacgt gggaacggaa tcacatcacg cacgttttgc acaccagtta
    22741 cgtaagcaat caggcgttca aaaccaagac cgaaacctga atgcggaaca gtaccgtagc
    22801 gacgcagatc gcgataccac cagtaatctt ctttattcag gcccatttcc agcatacgct
    22861 cgtccagcac gtccagacgt tcttcacgct gggagccacc aatgatctca ccgatgcccg
    22921 gagccagaac gtccatagcc gcaacggttt taccgtcttc gttaaggcgc atatagaacg
    22981 ctttaatatc tttcggatag tttttaacca ctaccggtgc tttaaagtgt tcttccgcca
    23041 gataacgctc atgctcagaa gagagatcaa cgccccagta aaccgggttt tcaaacttcc
    23101 tgccgcagtt ttcgagaatg gtcactgcgt cggtgtaatc cacctgcgca aaatcggctt
    23161 caatgaagcg ttccagacgt gaaacggcat ctttatctac gcgttcagcg aagaatttca
    23221 tgtcgtcagc gcgttcttcg agaaccgctt tgaagacata tttcagcatg gcttcagcca
    23281 gacccgcgat atcgttcagg ttagcaaacg ccacttccgg ctccagcatc cagaattccg
    23341 ccaggtgacg gctggtgttg gagttttcag cacggaaagt cgggccgaag gtataaattt
    23401 tggataatgc gcaagcgtag gtttcgccgt tcaactggcc agatacggtc aggaaagact
    23461 ctttaccaaa gaagtctttg tcgaaatcca ctttgccctg atcgttacgc ggcaggtttt
    23521 ccagatccag cgtagaaacg cggaacattt cgccagcccc ttcggtatca gacgcagtaa
    23581 tcagtggcgt tgaaacccag aagaatccct gctcgttaaa gaagcgatgc agcgcctgcg
    23641 ccagcgtatg gcgaacgcgc gcgacggcac caatcaggtt tgtgcgcgga cgcaggtgag
    23701 cgacttcacg cagatactca atgctgtggc gttttgccgc catcgggtaa gtgtctggat
    23761 cttcaaccca accagcaact tcaaccttgc tggcctgaat ttcaaattgt tgcccctggc
    23821 ccggcgacgc cacgacttta cccgtcacaa tgaccgagca gccggtggtc agacgcagga
    23881 cgtcttcatt gtagttgggc agagaattat tgatgacagc ctgtacagga tcaaagcagg
    23941 aaccgtcata aacggcgagg aaggagatgc cagcttttga atctcggcgg gtacgtaccc
    24001 atccgcgcac ggtgacttcg ctgtcaacgg ctacgcggcc ctggagtacg tcggctacag
    24061 gcacaacgct cataatattc tctctgttaa tagtcggaaa aaataaacac ttgtccaccc
    24121 gtaaatgggg ggatacctat gttacctggc atctgcaatc agacaagcag aattcgcaaa
    24181 tgcagcgaaa gatttcggaa ttaaaggtaa aaaagggagc cgattagctc cctgatgata
    24241 ttaactggct tttttaatat gcggaaggtc gaatgctttg cgcagtgccc gaacaaacgc
    24301 tttatcgtgg cagatagttt tgccagggct gtcagaaagt ttcgccaccg gtttaccgtt
    24361 acactcaacc aacttaatga caatattcag gggttttacc tgggggatat cgcaggtcag
    24421 gcgagtccca ataccaaaac ttaattgcac gcgggaagag aaatggcggt atagctcaac
    24481 cgctttgcgt aaatccagat tgtcagagaa aaccagcgtt ttactctgtg gatcaattcc
    24541 cagcttctca taatgtgcaa tggctttttc accccattca accgggtcgc cagagtcatg
    24601 acgcaggccc tgatagcgac tggcgaactc gacaccgaaa tcacgcagga aagcatccat
    24661 agtgatgcag tcggttaatg caatgccaag ttggtcggga tactcttcca gccaggcagc
    24721 aagtgcagct cgctggctgt tggctagatc cgggctgatt tgctgatgcg cctggaacca
    24781 ttcgtgtgcc tgtgttccca tcggcgtgag ggaaagccgg cgcgccagat catagttgct
    24841 ggtgcccacg aaccaggatt cctgttgcag acgcttaacg atggtttctt gtacttcgcg
    24901 agaaaaacgg cggcgggtgc caaaatccat cagatggaag cgcgacatat caagaccggc
    24961 ggttaatgcc gagaagtcga ctaatttgct ttccagcgtg tcgagggctt gcgcaacgtc
    25021 ggtctgcggt gagcgatagc gatgtaccat ttcactgata accgccagca aaggaacttc
    25081 ccagaggatg acttcacgcc acgggccgct taaacgaata tccagcttgc cattatcgtt
    25141 ggatacggtg acttgttccg ggttaaagcg gaactcgcgt aaccagttaa gatagtcggc
    25201 ctgaaagaaa ggcagggcag agagccactg atattcatca tcctgcaggc gcaggtgctg
    25261 catcgcctga acctgttcac gaatagcatc ggcataaata cccagcagat cgtcacctcg
    25321 gcaacgaaac tccgccgcga catgcacatc gtaatagtga tgaaacacgg cttgctgcat
    25381 atgcaactta taagcatctg tatccagcaa cgagtgcaga acaggagaag cgaattgtgt
    25441 cataggtgcg cagtagcgtc ctcgtacagg agcgtttagt acaataaaca tcttcaggaa
    25501 ctgctggagt ataccttgtt tcgcaattta ttgaaccccg atcacaccat atgccacctt
    25561 tctggtcgat ggcattacgc ggtgtatgtt ataaaaatgt agcaataaag gcgtttgtac
    25621 ctgaaaagat gaagattctg catagcgcga tttacgcaac aggaatagac tgaacaccag
    25681 actctataaa agatgctaaa ggttatttat gactcaacag ccacaagcca aataccgtca
    25741 cgattatcgt gcgccggatt accagattac tgatattgac ttgacctttg acctggacgc
    25801 gcaaaagacg gtcgttaccg cggtcagcca ggctgtccgt catggtgcat cagatgctcc
    25861 gcttcgtctt gatggcgaag atcttaaact ggtttctgtt catattaatg atgagccgtg
    25921 gaccgcctgg aaagaagaag agggcgcact ggtcatcagt aatttgccgg agcgttttac
    25981 gcttaagatc gttaatgaaa ttagcccggc gacgaatacc gcgctggaag ggctttatca
    26041 gtcaggcgat gcgctttgca cccagtgtga agccgaaggt ttccgccata ttacgtatta
    26101 tctcgaccgc ccggacgtgc tggcgcgttt taccaccaaa attattgccg ataaaaccca
    26161 atatcccttc ctgctttcca acggtaaccg cgttgcgcaa ggcgaactgg aaaacggacg
    26221 tcactgggta cagtggcagg acccgttccc taaaccgtgc tacctgtttg cgctggtggc
    26281 aggcgacttc gacgtgctgc gcgacacctt caccacgcgt tctggtcgcg aagttgcgct
    26341 ggagctgtac gtcgatcgcg gcaaccttga tcgtgcgccg tgggcgatga cctcgctgaa
    26401 aaactccatg aaatgggatg aagagcgctt cggcctggag tatgacctcg acatctatat
    26461 gatcgtcgcg gtggatttct tcaatatggg cgcaatggag aataagggtc tgaatatctt
    26521 taactccaaa tatgtgctgg cccgcaccga taccgccacc gacaaagatt acctcgatat
    26581 tgaacgcgtt atcggccatg aatatttcca taactggacc ggtaaccgag tgacctgccg
    26641 cgactggttc cagctcagcc tgaaagaagg tttaaccgtc ttccgcgatc aggagttcag
    26701 ctctgacctt ggttctcgcg cggtaaaccg tatcaacaac gtgcgcacca tgcgcggatt
    26761 gcagtttgcg gaagacgcca gcccgatggc gcacccgatc cgcccggata tggtcattga
    26821 gatgaacaac ttctacaccc tgaccgttta cgagaagggc gcggaagtca ttcgcatgat
    26881 ccacaccctg ctgggcgaag aaaacttcca gaaagggatg cagctctatt tcgagcgcca
    26941 tgatggcagc gcggcaacct gcgacgactt tgttcaggcg atggaagatg cgtcgaatgt
    27001 cgatctctct catttccgcc gttggtacag ccagtccggt acgccggttg tgaccgtcaa
    27061 agacgactat aatccggaaa ccgagcagta caccctgacc atcagccagc gtacgcctgc
    27121 tacgccggat caggcagaaa aacagccgct gcatattccg tttgccatcg aactgtatga
    27181 caacgaaggc aaagtgatcc cgttgcaaaa aggcggtcat ccggtgaatt cggtgctgaa
    27241 cgtcacccag gcggaacaga cctttgtctt tgataatgtc tactttcagc cggtgcctgc
    27301 gctgctgtgc gaattctctg cgccagtgaa actggaatat aagtggagcg atcagcaact
    27361 gaccttcctg atgcgccatg cgcgtaatga tttctcccgc tgggatgcgg cgcaaagcct
    27421 gctggcaacc tacatcaagc taaacgtcgc ccgtcatcag caagggcagc cgctgtctct
    27481 gccggtgcat gtggctgacg ctttccgcgc ggtgctgctc gatgagaaga ttgatccggc
    27541 gctggcggca gaaatcctga cgctgccttc tgtcaatgaa atggctgaac tgttcgatat
    27601 catcgacccg attgctattg ccgaagtacg cgaagcactc actcgtactc tggcgactga
    27661 actggcggat gagctgctgg ctatttacaa cgcgaattac cagagcgagt accgtgttga
    27721 gcatgaagat attgcgaaac gcactctgcg taatgcctgc ctgcgcttcc ttgcttttgg
    27781 tgaaacgcat ctggctgacg tgctggtgag caagcagttc cacgaagcga acaatatgac
    27841 cgatgcgctg gcggcgcttt ctgcggcggt tgccgcacaa ctgccatgcc gtgatgcgct
    27901 gatgcaggaa tacgacgata agtggcatct ggacggtctg gtgatggata aatggtttat
    27961 cctgcaagcc accagcccgg cggcgaatgt gctggagacg gtgcgcggtc tgttgcagca
    28021 tcgctcattt accatgagca acccgaaccg cattcgttcg ttgattggcg cgtttgcggg
    28081 cagcaacccg gcagcgttcc atgccgaaga tggcagcggt tatcagttcc ttgtggaaat
    28141 gcttaccgac ctcaacagcc gtaacccgca ggtggcatcg cgcctgattg agccgctgat
    28201 tcgcctgaaa cgttacgatg ccaaacgtca ggagaaaatg cgcgcggcgc tggagcagtt
    28261 gaaagggctg gaaaatctct ctggcgatct gtacgagaag atcaccaaag cactggcttg
    28321 ataaataacc gaatggcggc aatagcgccg ccattcgggg aatttacccc tgttttctca
    28381 ggcgaatttc agattcacca cgctgcatca ccctttgcaa cacttccgat tccagctccg
    28441 ctaatcgcac cgaccctaat cgacgcggac gggggatatc cactgtcaaa tccagaccaa
    28501 tttttccctc ttcaattaat aacacccggt cagccattgc taccgcttcg ctcacatcat
    28561 gcgtcaccag caaaacggta aaaccgtgct tctgccaaag cgacacaatc aaatcctgca
    28621 tctcgagtcg cgttaaagcg tccagcgcac cgagtggttc atcaagtaac aataaacccg
    28681 gtcgatgaat cagcgccctt gccagcgcca cacgttgttt ctgcccgcca gaaagtgcgg
    28741 caggccattc cccggcgcga ttctccagtc ctaccgcagc cagagcctga cgcgccgcat
    28801 cgcgccactg gcctttaagg cctaacccaa cgttatcaat caccgatttc cacggcagca
    28861 gacgcgcgtc ctgaaacatc attcgcgtat catcctgaat ctcagccagc ggtgtggtgc
    28921 ccgctaacac gtcgcctgcg gttggcgttt ccagacctgc cagcaggcgc agcagggtac
    28981 ttttgccacc accgctgcgg cccaccaccg ccacaaactg acctgccgga atatgtaaat
    29041 ccagttggtt caggacgata ttttccgcgt aatgtttgct tactgcattg agcaacaatg
    29101 gtgtgccctg gttcagacga gcagtattca taccgtggcc tccttcaaat gataagccgg
    29161 gttccagcgc aaccagagac gctctaacag ctgcgcgctg acgtcagcaa gtttgccgag
    29221 cagggcgtaa agaataatag cgactaccac cacgtccgtt tgcagaaact cccgcgcatt
    29281 catcgccaga taaccaatgc cggaattggc agaaatagtt tcggcaacga tcagcgtcag
    29341 ccacatcagg cccaaagcaa aacgcacacc gaccataatt gagggcaggg caccaggcag
    29401 gatcacatgg ataaacagcg gtatgccgga taatccatag ctacgtgcca tctccaccag
    29461 cccacggtcg atattacgga tgccatgcca ggtgttgata taaatgggga atagcgtacc
    29521 gagcgccacc agaaagattt ttgcggattc atcaatgcca aaccacaaaa tcaccagcgg
    29581 gatcagcgcc agatgcggca cgttgcgcaa catctgaatg gaggtatcca gcagccgttc
    29641 tccccagcgc gacagcccgc taatcagtcc cagaatcaac cccagcgatc cgccaattga
    29701 aaagccaatc aacgcccgcc aggagctgat cgccagatgc tgccacaatt cgccgctggc
    29761 agagagcgtc cagaacgcca tcaccactcc ttccggtgag ggcaaaatac gcgtcgatag
    29821 ccagccaacc gaggaggcca gttgccacac cgccacgatg cccaccggta aaaaccaggg
    29881 ggcaacgcgc aataaccact tcttcactgg cgttgccatc atcgtgttcc ttagctttgc
    29941 gcgacgttac gggggataaa atcattcgcc accgcttcgc cttgcggatt cagcggttgt
    30001 ggctggggaa tttccgggat ggcgacatcc agatgtggga acagtaactc gccgacccga
    30061 tacgcttctt ccagatgcgg gtagcccgaa agcacaaaac tgtcgatgcc aagtgcagcg
    30121 tattcgttga ttcgcgcggc gaccgtagga ccatcgccca ccagcgccgt cccggcaccg
    30181 ccgcgcacta agccaacacc cgcccataaa ttggggctaa tctccagatt gtcgcgcttg
    30241 ccgttatgta gcgccgccat tcgctgttgt cctacggaat ccgttcgggc gaatgcggcc
    30301 tgtgctttgg cgatagtttc atcatcaaga tgcgagatta accgctcggc ggcctgccac
    30361 gcttcgccgt tggtttcacg aacaatcaca tgcagacgaa taccgaaacg aattttgcgt
    30421 ccatgcgcgg cagctttcgc ccgcacttgt tcgattttct cttttaccag ttccggtggt
    30481 tcgccccagg tgaggtagag atcaacttgt tctgccgcca gctcctgggc gacatctgac
    30541 gatccgccaa agtaaagcgg aggatacggc tgttgaatcg gcgggaagag cagtttggct
    30601 ccgcgtacat gaatatgttt gccgttgaaa tccactgtct cgccaagcaa caaccgccgc
    30661 cagacttggg taaattctgc cgaggcttcg taacgctcgc tatgatcaag gaacacgccg
    30721 tcgcctgcca gttcttgggg atcgctgcct gtgaccaggt taaacaacgc acgtccgtta
    30781 gagagacggt caagcgtggc ggcctggcgg gcggcaacgg taggcgatgt cacactgggg
    30841 cgcagggcga caagaaactt cagccgttgt gtcaccggga tcatcgacgc cgccaccagc
    30901 cacgcatctt cacacgatcg ccccgtcggg atcagcacac cggtatagcc cagacgatcc
    30961 gccgcttgcg caatttgttg cagataaccg tgatcaaccg gacgtgaacc ttcttccgtt
    31021 cccagataat gcccgtcacc gtgggtcggt aaaaaccaga acatattcag actcataatt
    31081 gttttccttc cagttgagtg ggttgccaga tgcgctggcg aatatcgact tttttcggca
    31141 ccagacggtt ttcataaaac agatctgccg tttgctgctg taaggcggca acttcagcgc
    31201 tcaacggttt gatggtggtg ggaggacgat gatccagata cgaggcaata accggtgccg
    31261 gtaagcccat cgtttttgcc agtaaagcga tgctttgttc gcgctggctg cgggttaacg
    31321 catcggcctc actaaaggtt gccagtacgc cctgaataaa agcgccgttt ttttctgcat
    31381 acggacgggc tgccagataa aacgatccgg tttgattgag atcggtgccg tctttcaaca
    31441 cccgcacgcc gccctgtaat aatgcagcgg agtagtaggg atcccagata gcccaggcgt
    31501 caacgttacc ttgctggaac gcggcgcggg catcggcagg cgtcaggtaa gtgggctgga
    31561 tatccgtgaa tttaagcccg gcctggcgta gtgcgcgtag taaaagattg tgcgagctgg
    31621 aacctttctg aaaggcaact ttatgacctt taagatcggc tacggttttg attgggctgt
    31681 tatctgccac cagaatcact tcggctttgg gcttcggcgg ctcgacgccc acgtacacca
    31741 aatcagcccc ggcagcctgg gcaaagattg gcggaatatc cccggtactg ccgagatcaa
    31801 tactgccaac gtttaacgcc tccagcatct gtgggccagc cgggaactcc acccaggaga
    31861 tttttgtttg cggatagcgt ttttccagta attggtggct ttttgccagc accataccaa
    31921 tactgccttt ctgatagcca attcgtaacg cctctggcga agactccgcg gcaacagcaa
    31981 gtgtagagac gctaagtaaa cccgccagca ccagtttaat gagcttacgc atgggcatta
    32041 cctcgcagag acagtaggtc aggaacctga acatcgcggc ggtgcaacgc ctgacagaaa
    32101 gtttctagcg cggtatcaag acgggtttgc agatttggcg tgaatagcgg cttgtgctgg
    32161 taatcaatca cctgcgagtc atcggcaaac acgccgtgca ggatctcctg ggctttcaac
    32221 gcacttaaaa ctggatttag ggcataatcg accgccagta gatgagccac ggtgccgccc
    32281 gtcgccagcg gcaacacgac tttgccttgc agcgcgcgtt ctggcagcag gtcgaggagc
    32341 gttttcagtg caccggaata ggctgcttta tacacaggcg tggcgacaat cagcccatca
    32401 gcctgttgca gctgttcggt gaaggtcttg agtgccggac tatcgaaacg ggcataaagc
    32461 agatcttccg gggcgaagtt ttgcagattc cagtgataaa tctctacatc caggccattt
    32521 agtttttccc gcgcatattc cagcaaggag ctggagcgag aaggaaagcg aggacttcct
    32581 gccagggtga tgacacgcat actctctcct tataaccaat tgttcttgtt ttgttaacat
    32641 tgataacaat tcggtcagtc tgtcggagag acaagagaat gccaaatata aattttgtgt
    32701 atctttttct gaaaatgaac taaggattga gggatatata tcacgtgtat ttatttattg
    32761 gatattcaat agatgttgaa tcaatgaatt actcgttcag gtaataaaga aagattcatg
    32821 gaatctataa ataaaagatt tgtcacgtat tgagtaaaca ctactggttt aaatggcggt
    32881 catttgttgt tattattaag tccaatgtca tttgcaggca ttatcatatt tacgaaaaaa
    32941 tcaggatgat aacgatgaaa aaaaatgtat tggcggcgtt tataactgtg atatgtacaa
    33001 cttcaagtgt tatggcggct gatgataatg ccatcacgga tggctcggtt acgtttaatg
    33061 gtaaagtcat tgctcctgcc tgtacgctgg ttgctgcgac gaaagattcc gtggtgactt
    33121 taccagatgt tagtgcaacg aagttgcaaa gtaatggtca ggtttctggg gtgcaaactg
    33181 atgtgccaat tgcattagaa gattgtgata ttaccgtaac aaaaaatgca acgttcactt
    33241 ttaatggcac agcggatact acgcagatta cagcgtttgc taaccaggcc tcatctgagg
    33301 ctgctacaaa cgtggccctg caaatgtata tgaatgatgg tacaacggtc attacgccag
    33361 acacagaaac cgggaatatt ttgttgcaga gtggaaatca aacgctaact tttaaagtag
    33421 attatatcgc tacgggtaaa gcgacgtcag gtaatgtcaa tgcggtaaca aatttccata
    33481 ttaattatta ttaatatcag ccattaattt ttaattaatt aatacccttc cagtgtcctg
    33541 gaggggatat tcattttttt aagagtgact atttatgaaa acttgcataa caaagggaat
    33601 tgtgaccgta agtttaacgg caatattact cagctgttcg ttcacatggg cggcaggtaa
    33661 aggtggggtc ggacttgcag caacacgtct tgtttattcg gaaggtgaag agcaaatttc
    33721 actgggtgta cgtaacacca gtccggatgt tccttatctg attcagtcat gggtgatgac
    33781 accagataat aaaaagtcag cggactttat tattacacca ccgctatttg tgctgaatcc
    33841 ggcaaacgag aacctgctac gcattatgta cattggcgcg ccgttggcga aagacagaga
    33901 aactcttttc ttcaccagcg tacgggcagt cccttcaaca acgaagcggg aagaaggcaa
    33961 taccctgaag attgccacac aaagcgtcat caaacttttc tggcgaccaa aaggtttagc
    34021 gtatccctta gccgaggctc cggcgaaact acgttgcact tcgtcagctg acatggttac
    34081 ggtcagtaac ccaacacctt atttcattac cctgacagac ctgaaaatag gtggaaaagt
    34141 agttaaaaat caaatgattt ccccctttga taaataccaa ttctctctgc caatgggtgc
    34201 caaaaacagc agcgtaacgt accgaactat caatgactac ggggcggaaa cgccgcaact
    34261 caactgtaaa tcgtaattcg tcttcattta agagagcgag atgtatagaa ctcaacgaca
    34321 acacagcctg ttaagctctg gtggagtgcc atcgtttatt ggtgggctgg tggtgtttgt
    34381 gtcggcagcg ttcaatgcac aagctgaaac ctggttcgat ccagcctttt tcaaagatga
    34441 tccctcaatg gtggctgatt tgtctcgttt cgaaaaaggg caaaaaataa cgccaggggt
    34501 ttatcgagtc gatattgttc tgaatcagac aattgtagat acgcgcaacg tcaattttgt
    34561 tgagataacg ccagagaagg ggattgccgc ctgtttgacg actgaaagcc tggatgcaat
    34621 gggcgtgaat actgatgcgt ttccggcttt taaacaactg gataaacaag cgtgtgcgcc
    34681 attggcggaa attattccgg atgccagcgt aacttttaat gtgaataaac tccgtctgga
    34741 aatttcagta ccgcaaatcg ccatcaaaag taacgctcgt ggttatgtcc cccctgaacg
    34801 ttgggatgaa gggatcaacg cgctattact gggatattca tttagcgggg ctaacagtat
    34861 tcatagcagc gcaggtagtg attctggcga cagctatttt ctgaatttaa acagtggcgt
    34921 taatttaggc ccatggagat tgcgcaacaa ttcaacatgg agtcgcagta gtggccaaac
    34981 cgcagaatgg aagaatctca gcagctattt gcagcgggcg gtgattccat taaaaggcga
    35041 actgaccgtc ggtgatgatt atacggcagg cgattttttt gatagcgtca gctttcgtgg
    35101 cgtgcagctg gcgtcagatg acaacatgct gccagacagc ttgaaagggt ttgcgccagt
    35161 ggtgcgtggt atcgccaaaa gcaatgcaca ggtaacgatt aagcaaaatg gttacaccat
    35221 ctatcaaact tatgtttcgc ctggcgcttt tgagattagt gatctctact ctacgtcgtc
    35281 gagtggtgat ttgttggttg aaatcaaaga agctgacggt agtgtcaata gttacagtgt
    35341 gcccttttcc agcgtgccat tactccagcg tcagggacgc atcaaatatg cggtgacgct
    35401 ggcgaaatac agaaccaata gtaatgacca gcaagaaagt aaatttgctc aggccacgct
    35461 gcaatggggt gggccgaggg gaacgacctg gtatggcgga gggcaatatg ctgaatatta
    35521 ccgtgccgct atgtttggtc tgggttttaa cctcggcgat ttcggagcaa tttcgttcga
    35581 cgcgacccag gctaaaagta cgctggcaga ccaaagtgaa cataaagggc agtcatatcg
    35641 ttttctgtat gccaaaacgc tcaaccaatt gggcaccaac tttcaattga tgggctaccg
    35701 ctattcgacg tcgggtttct acaccctttc cgacactatg tataaacaca tggatggcta
    35761 cgaatttaat gacggtgatg atgaagatac gccaatgtgg tcgcgttatt acaatttgtt
    35821 ttacacaaaa cgtggcaaac tgcaggtcaa catctcccag caattaggcg agtacggttc
    35881 gttttattta agcggtagcc agcaaactta ctggcatacc gatcaacagg atcggctatt
    35941 acagtttggc tacaacacgc aaattaaaga tctctcgctg ggggtttcct ggaactacag
    36001 taagtcccgt ggtcaacctg acgctgacca ggtgtttgca cttaatttt
//
LOCUS       DAMZFT010000036        36023 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.036, whole genome shotgun sequence.
ACCESSION   DAMZFT010000036 DAMZFT010000000
VERSION     DAMZFT010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 36023)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36023)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36023
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.036"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(<1..1098)
                     /locus_tag="PN977_001893"
     CDS             complement(<1..1098)
                     /locus_tag="PN977_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HDI6041924.1"
                     /translation="MDSHTLIQALIYLGSAALIVPIAVRLGLGSVLGYLIAGCIIGPW
                     GLRLVTDAESILHFAEIGVVLMLFIIGLELDPQRLWKLRAAVFGGGALQMVICGGLLG
                     LFCMLLGLRWQVAELIGMTLALSSTAIAMQAMNERNLMVTQMGRSAFAVLLFQDIAAI
                     PLVAMIPLLAASSASTTMGAFALSALKVAGALVLVVLLGRYVTRPALRFVARSGLREV
                     FSAVALFLVFGFGLLLEEVGLSMAMGAFLAGVLLASSEYRHALESDIEPFKGLLLGLF
                     FIGVGMSIDFGTLIENPLRIVILLLGFLIIKIAMLWLIARPLQVPNKQRRWFAVLLGQ
                     GSEFAFVVFGAAQMANVLEPEWAKSLTLAVAL"
     gene            complement(1091..1621)
                     /gene="kefF"
                     /locus_tag="PN977_001894"
     CDS             complement(1091..1621)
                     /gene="kefF"
                     /locus_tag="PN977_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     oxidoreductase KefF"
                     /protein_id="HDI6041925.1"
                     /translation="MILIIYAHPYPHHSHANKRMLEQARTLEGVEIRSLYQLYPDFNI
                     DIAAEQEALSRADLIVWQHPMQWYSIPPLLKLWIDKVFSHGWAYGHGGTALHGKHLLW
                     AVTTGGGESHFEIGAHPGFDVLSQPLQATAIYCGLNWLPPFAMHCTFICDDETLEGQA
                     RHYKQRLLEWQEAHHG"
     gene            complement(1729..3060)
                     /locus_tag="PN977_001895"
     CDS             complement(1729..3060)
                     /locus_tag="PN977_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDI6041926.1"
                     /translation="MQPSRNFDDLKFSSIHRRILLWGSGGPFLDGYVLVMIGVALEQL
                     TPALKLDADWIGLLGAGTLAGLFVGTSLFGYISDKVGRRKMFLIDIIAIGVISVATMF
                     VSSPVELLVMRVLIGIVIGADYPIATSMITEFSSTRQRAFSISFIAAMWYVGATCADL
                     VGYWLYDVEGGWRWMLGSAAIPCLLILIGRFELPESPRWLLRKGRVKECEEMMIKLFG
                     EPVAFDEEQPQQTRFRDLFNRRHFPFVLFVAAIWTCQVIPMFAIYTFGPQIVGLLGLG
                     VGKNAALGNVVISLFFMLGCIPPMLWLNTAGRRPLLIGSFAMMTLALAVLGLIPDMGI
                     WLVVMAFAVYAFFSGGPGNLQWLYPNELFPTDIRASAVGVIMSLSRIGTIVSTWALPI
                     FINNYGISSTMLMGAGISLFGLLISVAFAPETRGMSLAQTSNMTIRGQRMG"
     gene            complement(3119..3406)
                     /gene="fixX"
                     /locus_tag="PN977_001896"
     CDS             complement(3119..3406)
                     /gene="fixX"
                     /locus_tag="PN977_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein FixX"
                     /protein_id="HDI6041927.1"
                     /translation="MTSPVNVDVKLGVNKFNVDEEHPHIVVKADADKQALELLVKACP
                     AGLYKKQDDGSVRFDYAGCLECGTCRILGLGSALEQWEYPRGTFGVEFRYG"
     gene            complement(3403..4689)
                     /locus_tag="PN977_001897"
     CDS             complement(3403..4689)
                     /locus_tag="PN977_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HDI6041928.1"
                     /translation="MSEDIFDAIIVGAGLAGSVAALVLAREGAQVLVIERGNSAGAKN
                     VTGGRLYAHSLEHIIPGFADSAPVERLITHEKLAFMTEKSAMTMDYCNGDETSPSQRS
                     YSVLRSKFDAWLMEQAEEADAQLITGIRVDNLVQRDGKVVGVEADGDVIEAKTVILAD
                     GVNSILAEKLGMAKRVKPTDVAVGVKELIELPKSVIEDRFQLQGNQGAACLFAGSPTD
                     GLMGGGFLYTNENTLSLGLVCGLHHLHDAKKSVPQMLEDFKQHPAVAPLIAGGKLVEY
                     SAHVVPEAGINMLPELVGDGVLIAGDAAGMCMNLGFTIRGMDLAIAAGEAAAKTVLSA
                     MKSDDFSKQKLAEYRQHLESGPLRDMRMYQKLPAFLDNPRMFNGYPELAVGVARDLFT
                     IDGSAPELMRKKILRHGKKVGFINLIKDGMKGVTVL"
     gene            complement(4740..5681)
                     /locus_tag="PN977_001898"
     CDS             complement(4740..5681)
                     /locus_tag="PN977_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HDI6041929.1"
                     /translation="MNTFSQVWVFSDTPSRLPELMNGAQALANQINTFVLNDADGAQA
                     IQLGANHVWKLSGKPDERMIEDYAGVMADTIRQHGADGLVLLPNTRRGKLLAAKLGYR
                     LKAAVSNDASTVSVQDGKATVKHMVYGGLAIGEERIATPYAVLTISSGTFDAAQPDAS
                     RTGETHTVEWQAPAVAITRTATQARQSNSVDLDKARLVVSVGRGIGSKENIALAEQLC
                     KAIGAELACSRPVAENEKWMEHERYVGISNLMLKPELYLAVGISGQIQHMVGANASQT
                     IFAINKDKNAPIFQYADYGIVGDAVKILPALTAALAR"
     gene            complement(5696..6466)
                     /locus_tag="PN977_001899"
     CDS             complement(5696..6466)
                     /locus_tag="PN977_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein"
                     /protein_id="HDI6041930.1"
                     /translation="MKIITCYKCVPDEQDIAVNNADGSLDFSKADAKISQYDLNAIEA
                     ACQLKQQAAEGQVTALSVGGKALTNAKGRKDVLSRGPDELIVVIDDQFEQALPQQTAS
                     ALAAAAQKAGFDLILCGDGSSDLYAQQVGLLVGEILNIPAVNGVSKIISLTADTLTVE
                     RELEDETETLSIPLPAVVAVSTDINSPQIPSMKAILGAAKKPVQVWSAADIGFNAEAA
                     WSEQQVAAPKQRERQRIVIEGDGEEQIAAFAENLRKVI"
     gene            6939..8453
                     /gene="caiT"
                     /locus_tag="PN977_001900"
     CDS             6939..8453
                     /gene="caiT"
                     /locus_tag="PN977_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine/gamma-butyrobetaine antiport BCCT
                     transporter"
                     /protein_id="HDI6041931.1"
                     /translation="MKNEKRKTGIEPKVFFPPLIIVGILCWLTVRDLDAANVVINAVF
                     SYVTNVWGWAFEWYMVVMLFGWFWLVFGPYAKKRLGNEPPEFSTASWIFMMFASCTSA
                     AVLFWGSIEIYYYISTPPFGLEPNSTGAKELGLAYSLFHWGPLPWATYSFLSVAFAYF
                     FFVRKMEVIRPSSTLVPLVGEKHAKGLFGTIVDNFYLVALIFAMGTSLGLATPLVTEC
                     MQWLFGIPHTLQLDAIIITCWIILNAICVACGLQKGVRIASDVRSYLSFLMLGWVFIV
                     SGASFIMNYFTDSVGMLLMYLPRMLFYTDPIAKGGFPQGWTVFYWAWWVIYAIQMSIF
                     LARISRGRTVRELCFGMVLGLTASTWILWTVLGSNTLLLMDKNIINIPNLIEQYGVAR
                     AIIETWAALPLSTATMWGFFILCFIATVTLVNACSYTLAMSTCREVRDGEEPPLLVRI
                     GWSILVGIIGIVLLALGGLKPIQTAIIAGGCPLFFVNIMVTLSFIKDAKQNWKD"
     gene            8484..9626
                     /gene="caiA"
                     /locus_tag="PN977_001901"
     CDS             8484..9626
                     /gene="caiA"
                     /locus_tag="PN977_001901"
                     /EC_number="1.3.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA dehydrogenase"
                     /protein_id="HDI6041932.1"
                     /translation="MDFNLNDEQELFVAGIRELMASENWEAYFAECDRDSVYPERFVK
                     ALADMGIDSLLIPEEHGGLDAGFVTLAAVWMELGRLGAPTYVLYQLPGGFNTFLREGT
                     QEQIDKIMAFRGTGKQMWNSAITEPGAGSDVGSLKTTYTRRNGKIYLNGSKCFITSSA
                     YTPYIVVMARDGASPDKPVYTEWFVDMSKPGIKVTKLEKLGLRMDSCCEITFDDVELD
                     EKDMFGREGNGFNRVKEEFDHERFLVALTNYGTAMCAFEDAARYANQRVQFGEAIGRF
                     QLIQEKFAHMAIKLNSMKNMLYEAAWKADNGTITSGDAAMCKYFCANAAFEVVDSAMQ
                     VLGGVGIAGNHRISRFWRDLRVDRVSGGSDEMQILTLGRAVLKQYR"
     gene            9755..10972
                     /gene="caiB"
                     /locus_tag="PN977_001902"
     CDS             9755..10972
                     /gene="caiB"
                     /locus_tag="PN977_001902"
                     /EC_number="2.8.3.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine CoA-transferase"
                     /protein_id="HDI6041933.1"
                     /translation="MDHLPMPKFGPLAGLRVVFSGIEIAGPFAGQMFAEWGAEVIWIE
                     NVAWADTIRVQPNYPQLSRRNLHALSLNIFKDEGREAFLKLMETTDIFIEASKGPAFA
                     RRGITDEVLWQHNPKLVIAHLSGFGQYGTEEYTNLPAYNTIAQAFSGYLIQNGDVDQP
                     MPAFPYTADYFSGLTATTAALAALHKVRETGKGESIDIAMYEVMLRMGQYFMMDYFNG
                     GEMCPRMTKGKDPYYAGCGLYKCADGYIVMELVGITQIAECFKDIGLAHLLGTPEIPE
                     GTQLIHRIECPYGPLVEEKLDAWLAAHTIAEVKERFAELNIACAKVLTVPELESNPQY
                     VARESITQWQTMDGRTCKGPNIMPKFKNNPGQIWRGMPSHGMDTAAILKNIGYSENDI
                     QELVSKGLAKVED"
     gene            11046..12599
                     /gene="caiC"
                     /locus_tag="PN977_001903"
     CDS             11046..12599
                     /gene="caiC"
                     /locus_tag="PN977_001903"
                     /EC_number="6.2.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetaine/carnitine-CoA ligase"
                     /protein_id="HDI6041934.1"
                     /translation="MDIIGGQHLRQMWDDLADVYGHKTALICESSGGVVNRYSYLELN
                     QEINRTANLFYTLGIRKGDKVALHLDNCPEFIFCWFGLAKIGAIMVPINARLLREESA
                     WILQNSQACLLVTSAQFYPMYQQIQQEDATQLRHICLTDVALPADDGVSSFTQLKNQQ
                     PATLCYAPPLSTDDTAEILFTSGTTSRPKGVVITHYNLRFAGYYSAWQCALRDDDVYL
                     TVMPAFHIDCQCTAAMAAFSAGATFVLVEKYSARAFWGQVQKYRATITECIPMMIRTL
                     MVQPPSANDQQHRLREVMFYLNLSAQEKDAFCERFGVRLLTSYGMTETIVGIIGDRPG
                     DKRRWPSIGRAGFCYEAEIRDDHNRPLPAGEIGEICIKGVPGKTIFKEYFLNPKATAK
                     VLEADGWLHTGDTGYRDEEGFFYFVDRRCNMIKRGGENVSCVELENIIATHPKIQDIV
                     VVGIKDSIRDEAIKAFVVLNEGETLSEEEFFRFCEQNMAKFKVPSYLEIRKDLPRNCS
                     GKIIRKNLK"
     gene            12708..13493
                     /gene="caiD"
                     /locus_tag="PN977_001904"
     CDS             12708..13493
                     /gene="caiD"
                     /locus_tag="PN977_001904"
                     /EC_number="4.2.1.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA hydratase"
                     /protein_id="HDI6041935.1"
                     /translation="MSESLHLTRNGSILEITLDRPKANAIDAKTSFEMGEVFLNFRDD
                     PQLRVAIITGAGEKFFSAGWDLKAAAEGEAPDADFGPGGFAGLTEIFNLDKPVIAAVN
                     GYAFGGGFELALAADFIVCADNASFALPEAKLGIVPDSGGVLRLPKILPPAIVNEMVM
                     TGRRMGAEEALRWGVVNRVVSQAELMDNARELAQQLVNSAPLAIAALKEIYRTTSEMP
                     VEEAYRYIRSGVLKNYPSVLHSEDAIEGPLAFAEKRDPVWKGR"
     gene            13499..14089
                     /gene="caiE"
                     /locus_tag="PN977_001905"
     CDS             13499..14089
                     /gene="caiE"
                     /locus_tag="PN977_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine operon protein CaiE"
                     /protein_id="HDI6041936.1"
                     /translation="MSYYAFEGLIPVVHPTAFVHPSAVLIGDVIVGAGVYIGPLASLR
                     GDYGRLLVQAGANIQDGCIMHGYCDTDTIVGENGHIGHGAILHGCVIGRDALVGMNSV
                     IMDGAVIGEESIVAAMSFVKAGFRGEKRQLLMGTPARAVRSVSDDELHWKRLNTKEYQ
                     DLVGRCHASLHETQPLRQMEGNRPRLQGTTDVAPKR"
     gene            complement(14208..14603)
                     /gene="caiF"
                     /locus_tag="PN977_001906"
     CDS             complement(14208..14603)
                     /gene="caiF"
                     /locus_tag="PN977_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414576.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine metabolism transcriptional regulator
                     CaiF"
                     /protein_id="HDI6041937.1"
                     /translation="MCEGYVEKPLYLLIAEWMMAENRWVIAREISIHFDIEHSKAVNT
                     LTYILSEVTEISCEVKMIPNKLEGRGCQCQRLVKVVDIDEQIYARLRNNSREKLVGVR
                     KTPRIPAVPLTELNREQKWQMMLSKSMRR"
     gene            complement(14864..18085)
                     /gene="carB"
                     /locus_tag="PN977_001907"
     CDS             complement(14864..18085)
                     /gene="carB"
                     /locus_tag="PN977_001907"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HDI6041938.1"
                     /translation="MPKRTDIKSILILGAGPIVIGQACEFDYSGAQACKALREEGYRV
                     ILVNSNPATIMTDPEMADATYIEPIHWEVVRKIIEKERPDAVLPTMGGQTALNCALEL
                     ERQGVLEEFGVTMIGATADAIDKAEDRRRFDVAMKKIGLETARSGIAHTMEEALAVAA
                     EVGFPCIIRPSFTMGGSGGGIAYNREEFEEICARGLDLSPTKELLIDESLIGWKEYEM
                     EVVRDKNDNCIIVCSIENFDAMGIHTGDSITVAPAQTLTDKEYQIMRNASMAVLREIG
                     VETGGSNVQFAVNPKNGRLIVIEMNPRVSRSSALASKATGFPIAKVAAKLAVGYTLDE
                     LMNDITGGRTPASFEPSIDYVVTKIPRFNFEKFAGANDRLTTQMKSVGEVMAIGRTQQ
                     ESLQKALRGLEVGATGFDPKVSLDDPEALTKIRRELKDAGAERIWYIADAFRAGLSVD
                     GVFNLTNIDRWFLVQIEELVRLEEKVAEVGITGLNADFLRQLKRKGFADARLAKLAGV
                     REAEIRKLRDQYDLHPVYKRVDTCAAEFATDTAYMYSTYEEECEANPSTDREKIMVLG
                     GGPNRIGQGIEFDYCCVHASLALREDGYETIMVNCNPETVSTDYDTSDRLYFEPVTLE
                     DVLEIVRIEKPKGVIVQYGGQTPLKLARALEAAGVPVIGTSPDAIDRAEDRERFQHAV
                     ERLKLKQPANATVTTIEMAVEKAKEIGYPLVVRPSYVLGGRAMEIVYDEADLRRYFQT
                     AVSVSNDAPVLLDHFLDDAVEVDVDAICDGEMVLIGGIMEHIEQAGVHSGDSACSLPA
                     YTLSQEIQDVMRQQVQKLAFELQVRGLMNVQFAVKNNEVYLIEVNPRAARTVPFVSKA
                     TGVPLAKVAARVMAGKTLAEQGVTKEVIPPYYSVKEVVLPFNKFPGVDPLLGPEMRST
                     GEVMGVGRTFAEAFAKAQLGSNSTMKKHGRALLSVREGDKERVVDLAAKLLKQGFELD
                     ATHGTAIVLGEAGINPRLVNKVHEGRPHIQDRIKNGEYTYIINTTSGRRAIEDSRVIR
                     RSALQYKVHYDTTLNGGFATAMALNADATEKVISVQEMHAQIK"
     gene            complement(18103..19251)
                     /gene="carA"
                     /locus_tag="PN977_001908"
     CDS             complement(18103..19251)
                     /gene="carA"
                     /locus_tag="PN977_001908"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HDI6041939.1"
                     /translation="MIKSALLVLEDGTQFHGRAIGATGSAVGEVVFNTSMTGYQEILT
                     DPSYSRQIVTLTYPHIGNVGTNDADEESSQVHAQGLVIRDLPLIASNFRNTEDLSSYL
                     KRHNIVAIADIDTRKLTRLLREKGAQNGCIIAGDNPDAALALEKARAFPGLNGMDLAK
                     EVTTAETYSWTQGSWTLTGGLPEAKKEDELPFHVVAYDFGAKRNILRMLVDRGCRLTI
                     VPAQTSAEDVLKMNPDGIFLSNGPGDPAPCDYAITAIQKFLETDIPVFGICLGHQLLA
                     LASGAKTVKMKFGHHGGNHPVKDVEKNVVMITAQNHGFAVDEATLPANLRVTHKSLFD
                     GTLQGIHRTDKPAFSFQGHPEASPGPHDAAPLFDHFIELIEQYRKTAK"
     gene            complement(19707..20528)
                     /gene="dapB"
                     /locus_tag="PN977_001909"
     CDS             complement(19707..20528)
                     /gene="dapB"
                     /locus_tag="PN977_001909"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HDI6041940.1"
                     /translation="MHDANIRVAIAGAGGRMGRQLIQAALALEGVQLGAALEREGSSL
                     LGSDAGELAGAGKTGVTVQSSLDAIKDDFDVFIDFTRPEGTLNHLAFCRQHGKGMVIG
                     TTGFDEAGKQAIRDAAADIAIVFAANFSVGVNVMLKLLEKAAKVMGDYTDIEIIEAHH
                     RHKVDAPSGTALAMGEAIAHALDKDLKDCAVYSREGHTGERVPGTIGFATVRAGDIVG
                     EHTAMFADIGERLEITHKASSRMTFANGAVRSALWLSGKESGLFDMRDVLDLNNL"
     gene            complement(20695..21609)
                     /gene="rihC"
                     /locus_tag="PN977_001910"
     CDS             complement(20695..21609)
                     /gene="rihC"
                     /locus_tag="PN977_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside hydrolase RihC"
                     /protein_id="HDI6041941.1"
                     /translation="MRLPIFLDTDPGIDDAVAIAAAIFAPELDLQLMTTVAGNVSVEK
                     TTRNALQLLHFWNAEIPLAQGAAVPLVRAPRNAASVHGESGMAGYDFVEHNRSPLDKP
                     AFLAIRDALMRAPEPVTLVAIGPLTNIALLLSQCPECKQYIRRLVIMGGSAGRGNCTP
                     NAEFNIAADPEAAACVFRSGIEIVMCGLDVTNQAILTPDYLATLPELNRTGKMLHALF
                     SHYRSGSMQSGLRMHDLCAIAWLVRPDLFTLKPCFVAVETQGEFTSGTTVVDIDGCLS
                     KPANVQVALDLDVKGFQQWVAEVLALAS"
     gene            complement(21675..22625)
                     /gene="ispH"
                     /locus_tag="PN977_001911"
                     /gene_synonym="lytB"
     CDS             complement(21675..22625)
                     /gene="ispH"
                     /locus_tag="PN977_001911"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HDI6041942.1"
                     /translation="MQILLANPRGFCAGVDRAISIVENALAIYGAPIYVRHEVVHNRY
                     VVDSLRERGAIFIEQISEVPDGAILIFSAHGVSQAVRNEAKSRDLTVFDATCPLVTKV
                     HMEVARASRRGEESILIGHAGHPEVEGTMGQYSNPEGGMYLVESPDDVWKLTVKNEEK
                     LSFMTQTTLSVDDTSDVIDALRKRFPKIVGPRKDDICYATTNRQEAVRALAEQAEVVL
                     VVGSKNSSNSNRLAELAQRMGKRAFLIDDAKDIQEEWVKEVKCVGVTAGASAPDILVQ
                     NVVARLQQLGGGEAIPLEGREENIVFEVPKELRVDIREVD"
     gene            complement(22627..23076)
                     /gene="fkpB"
                     /locus_tag="PN977_001912"
     CDS             complement(22627..23076)
                     /gene="fkpB"
                     /locus_tag="PN977_001912"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HDI6041943.1"
                     /translation="MSESVQSNSAVLVHFTLKLDDGTTAESTRNNGKPALFRLGDASL
                     SEGLEQHLLGLKVGDKTTFSLEPDAAFGVPSPDLIQYFSRREFMDAGEPEIGAIMLFT
                     AMDGSEMPGVIREINGDSITVDFNHPLAGQTVHFDIEVLEIDPALEA"
     gene            complement(23164..23658)
                     /gene="lspA"
                     /locus_tag="PN977_001913"
     CDS             complement(23164..23658)
                     /gene="lspA"
                     /locus_tag="PN977_001913"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HDI6041944.1"
                     /translation="MSQSICSTGLRWLWLVVVVLIIDLGSKYLILQNFALGDTVPLFP
                     SLNLHYARNYGAAFSFLADSGGWQRWFFAGIAIGISVILAVMMYRSKATQKLNNIAYA
                     LIIGGALGNLFDRLWHGFVVDMIDFYVGDWHFATFNLADTAICVGAALIVLEGFLPSK
                     AKKQ"
     gene            complement(23658..26474)
                     /gene="ileS"
                     /locus_tag="PN977_001914"
     CDS             complement(23658..26474)
                     /gene="ileS"
                     /locus_tag="PN977_001914"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HDI6041945.1"
                     /translation="MSDYKSTLNLPETGFPMRGDLAKREPGMLARWTDDDLYGIIRAA
                     KKGKKTFILHDGPPYANGSIHIGHSVNKILKDIIVKSKGLSGYDSPYVPGWDCHGLPI
                     ELKVEQEYGKPGEKFTAAEFRAKCREYAATQVDGQRKDFIRLGVLGDWSHPYLTMDFK
                     TEANIIRALGKIIGNGHLHKGAKPVHWCVDCRSALAEAEVEYYDKTSPSIDVAFQAVD
                     QDALKAKFAVSNVNGPISLVIWTTTPWTLPANRAISIAPDFDYVLVQIDGQAVILAKD
                     LVESVMQRIGVTDYTILGTVKGAELELLRFTHPFMGFDVPAILGDHVTLDAGTGAVHT
                     APGHGPDDYVIGQKYGLETANPVGPDGTYLPGTYPTLDGVNVFKANDIVVALLQEKGA
                     LLHVEKMQHSYPCCWRHKTPIIFRATPQWFVSMDQKGLRAQSLKEIKGVQWIPDWGQA
                     RIESMVANRPDWCISRQRTWGVPMSLFVHKDTEELHPRTLELMEEVAKRVEVDGIQAW
                     WDLDAKEILGDEADQYVKVPDTLDVWFDSGSTHSSVVDVRPEFAGHAADMYLEGSDQH
                     RGWFMSSLMISTAMKGKAPYRQVLTHGFTVDGQGRKMSKSIGNTVSPQDVMNKLGADI
                     LRLWVASTDYTGEMAVSDEILKRAADSYRRIRNTARFLLANLNGFDPAKDMVKPEEMV
                     VLDRWAVGCAKAAQEDILKAYEAYDFHEVVQRLMRFCSVEMGSFYLDIIKDRQYTAKA
                     DSVARRSCQTALYHIAEALVRWMAPILSFTADEVWGYLPGEREKYVFTGEWYEGLFGL
                     ADSEAMNDAFWDELLKVRGEVNKVIEQARADKKVGGSLEAAVTLYAEPELAAKLTALG
                     DELRFVLLTSGATVADYNDAPADAQQSEVLKGLKVALSKAEGEKCPRCWHYTQDVGKV
                     AEHAEICGRCVSNVAGDGEKRKFA"
     gene            complement(26517..27458)
                     /gene="ribF"
                     /locus_tag="PN977_001915"
     CDS             complement(26517..27458)
                     /gene="ribF"
                     /locus_tag="PN977_001915"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HDI6041946.1"
                     /translation="MKLIRGIHNLSQAPQEGCVLTIGNFDGVHRGHRALLQGLQEEGR
                     KRNLPVMVMLFEPQPLELFATDKAPARLTRLREKLRYLAECGVDYVLCVRFDRRFAAL
                     TAQNFISDLLVKHLRVKFLAVGDDFRFGAGREGDFLLLQKAGAEYGFDITSTQTFCEG
                     GVRISSTAVRQALADDNLALAESLLGHPFAISGRVVHGDELGRTIGFPTANVPLRRQV
                     SPVKGVYAVEVLGLGEKPLPGVANIGTRPTVAGIRQQLEVHLLDVAMDLYGRHIQVVL
                     RKKIRNEQRFASLDELKAQIARDELTAREFFGLTKPA"
     gene            complement(27466..27684)
                     /locus_tag="PN977_001916"
     CDS             complement(27466..27684)
                     /locus_tag="PN977_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2575 family protein"
                     /protein_id="HDI6041947.1"
                     /translation="MCRHSLRSDGAGFYQLAGCEYSFSAIKIAAGGQFLPVICAMAMK
                     SHFFLISVLNRRLTLTAVQGILGRFSLF"
     gene            27787..28050
                     /gene="rpsT"
                     /locus_tag="PN977_001917"
     CDS             27787..28050
                     /gene="rpsT"
                     /locus_tag="PN977_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006117633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HDI6041948.1"
                     /translation="MANIKSAKKRAIQSEKARKHNASRRSMMRTFIKKVYAAIEAGDK
                     AAAQKAFNEMQPIVDRQAAKGLIHKNKAARHKANLTAQINKLA"
     gene            28458..29831
                     /locus_tag="PN977_001918"
     CDS             28458..29831
                     /locus_tag="PN977_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003018943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDI6041949.1"
                     /translation="MSSVIETTQSTSSDSLPLIQRISYGSLDVAGNLLYCFGSTYILY
                     FYTDVAGISLAVAGIILLLARIVDGIDAPVWGIIIDKTHSRYGKCRPWFLWLPLPFAV
                     FSALSFWSPDISMTGKAVYAAISYMLASILFTGLNTPLSAILPLMTLSPKERLVLNSY
                     RMTGGQIGVLLMNATALPLVAFLGNGNDKAGFIYTAIVFAVISCALTLFAFKNIRELD
                     TDKIQQEPRLPMKKSFSAMKGNWPWLLMVVANLIFWIALQQRNTTIVYYLTYNLDRKD
                     LVPLVNSLATIQILFIIAIPFFSRYLTKTWIWITGLLVAMLGGGLMWLAADSIPLMIA
                     AWVLANIGSGIACSMPFAMLGFAVDFGRWKTGIKATGILIAFGSTFCIKMGSGIGTAF
                     AAFIMDSFGYIPNQQQTAAGLEGISLAFIWVPALLFALAAVPLLFFRQYEAMEGRIQH
                     DLQAHNR"
     gene            29871..31910
                     /locus_tag="PN977_001919"
     CDS             29871..31910
                     /locus_tag="PN977_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 31 protein"
                     /protein_id="HDI6041950.1"
                     /translation="MSFIKQDPHRLVWQQDDRYLWIEAWGENSLRVRSGRHLPVMRNE
                     NWALTQDPGDAVAHITWDEKQATLRNGKITAIVNLQGQLSFWRNDDKCLLQEFWRQRG
                     EIGEDESAHGQYVSALNLQAREFKPIPGGKFTIKARFEANDGEKLFGMGQYQQPNLDL
                     KGCMLELAQRNSQASVPFLLSNQGYGFLWNNPAIGRVTFAQNGTEWVAEVSEQLDYWI
                     TAGDTPAEISEAYARVTGTPPMMPDYAMGFWQCKLRYRNQQELLEVARGYKQRNLPIS
                     VIVIDFFHWPNQGDWMFDLRDWPDPDAMIAELKEMGIELMVSFWPTVDNRTESYREMK
                     ENGWLVHTERGLPINMDFLGNTTFFDATHPGAREYVWNKAKRNYYDKGVKLFWLDEAE
                     PEFGVYDYDNYRYYAGPVLEVGNIYPRMYAKTFFDGMQAAGEKQVINLLRCAWAGSQK
                     YGALVWSGDIHSSFRSLRNQFAAGLNMGIAGIPWWTTDIGGFHGGNIHDPKFHELLIR
                     WFQWGVFSPVMRLHGNRDPQVLPEQPYRDGIAQCPTGAPNEVWSYGEETGEILTSCLQ
                     LREKLKPYISKIMAETHEKNSPVMRTMFFEFPDQAESWNIYDQYCFGPDLLVAPVMHE
                     GTRSRDVWLPAGETWIDFYTHEHYAGGQKLHHCAPLHQIPVFIREKGKYRQLLPV"
     gene            complement(31968..32867)
                     /gene="nhaR"
                     /locus_tag="PN977_001920"
     CDS             complement(31968..32867)
                     /gene="nhaR"
                     /locus_tag="PN977_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional activator NhaR"
                     /protein_id="HDI6041951.1"
                     /translation="MSHINYNHLYYFWHVYKEGSVVGAAEALYLTPQTITGQIRALEE
                     RLQGKLFKRKGRGLEPSELGELVYRYADKMFTLSQEMLDIVNYRKESNLLFDVGVADA
                     LSKRLVSSVLNAAVVEGEPIHLRCFESTHEMLLEQLSQHKLDMIISDCPIDSTQQEGL
                     FSVRIGECGVSFWCTNPPPEKPFPACLEERRLLIPGRRSMLGRKLLNWFNSQGLNVEI
                     LGEFDDAALMKAFGAMHNAIFVAPTLYAYDFYADKTVVEIGRVENVMEEYHAIFAERM
                     IQHPAVQRICNTDYSSLFSPASR"
     gene            complement(32933..34099)
                     /gene="nhaA"
                     /locus_tag="PN977_001921"
     CDS             complement(32933..34099)
                     /gene="nhaA"
                     /locus_tag="PN977_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HDI6041952.1"
                     /translation="MKHLHRFFSSDASGGIILIIAAILAMIMANSGATSGWYHDFLET
                     PVQLRVGSLEINKNMLLWINDALMAVFFLLVGLEVKRELMQGSLASLRQAAFPVIAAI
                     GGMIVPALLYLAFNYADPITREGWAIPAATDIAFALGVLALLGSRVPLALKIFLMALA
                     IIDDLGAIIIIALFYTNDLSMASLGVAAVAIAVLAVLNICGVRRTGVYILVGVVLWTA
                     VLKSGVHATLAGVIVGFFIPLKEKHGRSPAKRLEHVLHPWVAYLILPLFAFANAGVSL
                     QGVTLDGLTSILPLGIIAGLLIGKPLGISLFCWLALRLKLAHLPEGTTYQQIMAVGIL
                     CGIGFTMSIFIASLAFGSVDPELINWAKLGILVGSISSAVIGYSWLRVRLRPSV"
     gene            complement(34351..35595)
                     /locus_tag="PN977_001922"
     CDS             complement(34351..35595)
                     /locus_tag="PN977_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001356890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HDI6041953.1"
                     /translation="MFTNVNVDCCKTPGCKNLGLLNSQDYIAQGKNILCRECGYLFPV
                     ISERSLNIYRNRVNHSWRGLVPQCSACGSTSLKKYGYSAQGQRRMYCHHCSRTFITLD
                     HVNTTPRRTHLALMIDQGASLADIRKLLLLNSTGLNRELMKLAREVNYKESYQYSSAS
                     DISLSTRAFRVKFNGSNNYLYSLVTAEEQSGRVVAISTNYSPSAVESHYQYASSYEER
                     LSPGTLAHHVQRKELLTMRRDTLFDIDYGRAILHQNDPGMLVKPVLPAYRHFELVRAL
                     TEAYSINIQHYLDHECFILGGCLMANLQHIHQGRCHISFVKERGEKTTHYDTPPRLFL
                     SGGVRNNVWRTFSARDYSMAVCNLTGNKKANEMRYSTLASATRFINFLESHPFLSSLN
                     RMSPANVVSTLDIFKHLWNKQL"
     gene            complement(35725..>36023)
                     /locus_tag="PN977_001923"
     CDS             complement(35725..>36023)
                     /locus_tag="PN977_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="arylsulfatase"
                     /protein_id="HDI6041954.1"
                     /translation="ALKREKPLIFGIDMPFQDDPTDEWAIRDGDWKMIIDRNNKPKYL
                     YNLKTDRFETINQIGKNPDIEKQMYGKFLKYKADIDNDSLMKARGDKPEAVTWG"
BASE COUNT         8911 a         9628 c         8945 g         8539 t
ORIGIN      
        1 cagcgccacc gccagggtca gcgatttcgc ccactccggc tccagcacat tcgccatttg
       61 cgccgcgcca aacaccacaa aagcaaactc actaccctgc cctaacaaca ccgcaaacca
      121 gcgacgctgt ttattcggca cctgcaacgg tcgggcaatc agccacagca tggcgatttt
      181 gatgatgagg aaaccgagca gcagaatgac aatacgcaat gggttttcaa tcagcgtgcc
      241 aaagtctatc gacatgccaa cgccaatgaa aaacagcccc agcagcaaac ctttaaaggg
      301 ttcgatatcg ctttccagcg catgacgata ttccgagctt gccagcagta cgcccgccag
      361 aaacgcgccc atcgccatcg acaagccgac ctcttccagc agcaaaccaa agccaaacac
      421 gaggaataac gccacggcac taaacacttc ccgcaagcca gagcgggcta caaaacgcag
      481 cgccggacgc gtgacatagc gccccagcaa taccaccagc accagcgcac ccgccacttt
      541 taacgccgag agagcaaatg cgcccatcgt cgtcgaggca ctgctcgccg ccagtagcgg
      601 gatcatcgcc accagcggga tcgccgcgat atcctggaac agcagcaccg caaaggcact
      661 gcggcccatc tgcgtcacca tcagattacg ttcattcatc gcctgcatgg caatcgccgt
      721 agaggagagc gccagcgtca tgccgatcag ttccgcgacc tgccagcgca gcccaagcaa
      781 catacagaac agccccagca ggccgccgca aatcaccatc tgcaatgcgc caccaccgaa
      841 caccgccgca cgcagcttcc acagcctttg tggatcgagt tcgaggccga taataaacag
      901 catcagcacc acgccaatct cggcaaagtg caggatagat tcggcatcgg tcaccagtcg
      961 cagcccccac gggccaataa tacaacccgc gatcaggtag ccaagtaccg atcccagacc
     1021 aagacgtacc gcaatgggga caatcagcgc cgccgaaccg agataaatca gcgcctgaat
     1081 cagcgtatgg ctatccatga tgtgcctcct gccattccag cagacgttgc ttatagtgac
     1141 gcgcctgtcc ttcgagggtt tcgtcgtcac aaataaaggt gcagtgcatg gcaaacggtg
     1201 gcagccagtt cagcccgcag tagattgccg tcgcctgtag cggctgcgac agcacatcaa
     1261 agcccggatg cgcaccaatc tcaaaatggc tttccccacc gccggtcgtc accgcccaca
     1321 gcaaatgttt gccatgcagc gccgtgccgc cgtgaccgta agcccagccg tgcgagaaga
     1381 ctttatcgat ccaaagtttg aggagcggtg gaatgctgta ccactgcatc gggtgctgcc
     1441 agacgatcag atcggcgcga gacagcgcct cctgctcggc ggcaatatcg atattgaagt
     1501 caggatagag ttgataaaga gagcgaattt cgacgccttc cagcgtcctt gcctgttcaa
     1561 gcatccgttt attcgcatgg gaatgatgcg gatacggatg cgcataaatt ataagaatca
     1621 taggtagccc gcgtgattac gcctttgttt tcccacaaag tgtagtcata tattcacgcg
     1681 gcttatagtc aataatattg attcagaaaa gagagaaatc tgaacaaatt accccattct
     1741 ctgcccgcgg atcgtcatat tgctggtctg cgccagtgac atccctcgag tctccggagc
     1801 aaacgctacg gaaatcaaca agccaaatag cgagataccc gcccccatca gcatggtgct
     1861 actgatgccg taattattga taaagattgg cagtgcccag gtcgaaacaa tggtgccaat
     1921 acggcttaag gacataatca cgcccacggc agaggcgcgg atatccgtcg ggaagagttc
     1981 attaggatag agccactgca aattacccgg cccgccagag aaaaaggcat acaccgcaaa
     2041 tgccatcact accagccaga tccccatatc cgggatcagc cccaaaaccg ccagcgccag
     2101 cgtcatcatg gcaaaactgc caatcaacaa tggacgccgt ccggcggtgt ttaaccacag
     2161 catcggcgga atacagccaa gcataaagaa caggctaatc accacattcc ccaacgccgc
     2221 gtttttgcca actcccaatc ccaacaaacc aacgatttgc gggccaaagg tgtaaatggc
     2281 gaacatgggg atcacctggc aggtccagat ggcggcaaca aacagaacaa aaggaaaatg
     2341 gcggcgatta aacagatcgc gaaaacgggt ttgctgcggc tgctcttcat cgaaagccac
     2401 cggttcgcca aacagtttta tcatcatctc ttcgcactct tttactcgcc ctttgcgtaa
     2461 caaccagcgg ggagattcag gcagttcgaa tcgaccaatc aaaatcaaca aacaggggat
     2521 cgctgcgcta cccagcatcc agcgccaacc accttccaca tcataaagcc agtaaccgac
     2581 cagatcggcg caggtcgcgc cgacatacca catcgcggca ataaagctga tggaaaacgc
     2641 ccgctgacgg gtactggaga actcggtgat cattgaggtg gcgatgggat aatccgcacc
     2701 gatgacaatg ccgataagta cccgcatcac caacagttcg acgggggatg agacaaacat
     2761 cgtcgccaca gatatcacgc cgatggcgat gatatcaatg aggaacattt tgcgccgtcc
     2821 gactttatcg gagatatagc caaacagcga tgtgccaacg aacagcccgg cgagcgttcc
     2881 cgcgcccagc aagccaatcc agtcagcgtc cagtttcagc gccggagtca gttgctccag
     2941 cgccacgcca atcattacca gtacataacc atccagaaac ggaccaccgc ttccccacag
     3001 caaaatgcgg cggtgaatag aggagaattt aagatcgtca aagtttctgg acggttgcat
     3061 gggttattcc tgtttttttg caatgagagt cagtacggcg ttacgtatca aaccaacatc
     3121 agccgtaacg gaactccaca ccaaaggtgc cgcgcgggta ttcccactgt tccagcgccg
     3181 accccagccc cagaatgcga caggtgccgc actccagaca tccggcgtaa tcgaagcgca
     3241 cactgccgtc atcctgcttc ttgtacagac ctgcggggca cgctttcacc agcagctcca
     3301 gcgcctgttt atcggcatcg gccttcacaa caatgtgcgg atgctcttca tcgacattga
     3361 atttattgac gcccagtttg acgtccacat tgacgggaga agtcataaaa cggtcactcc
     3421 tttcatgcca tccttgatca gattgatgaa gcccactttc ttgccgtggc ggaggatttt
     3481 cttgcgcatc agttccggcg cactgccgtc aatggtgaac aggtcacgcg ccacgcccac
     3541 cgccagttcc gggtagccgt taaacatgcg tgggttatca aggaacgccg gtagtttctg
     3601 gtacatgcgc atatcgcgca gcgggccact ctcaagatgc tgacgatatt ccgcgagttt
     3661 ttgcttactg aaatcgtcgc ttttcatcgc tgaaagcaca gtttttgctg ctgcttcccc
     3721 ggcggcaatc gccagatcca taccgcgaat ggtaaaaccg aggttcatac acattccggc
     3781 ggcatcaccg gcaatcagta cgccgtcacc aaccaactcc ggcagcatgt tgatgcctgc
     3841 ttccggcact acgtgagcgg aatattccac cagcttgccg cctgcgatca gcggtgcaac
     3901 ggccggatgt tgtttgaaat cttccagcat ttgcggcacc gatttttttg cgtcatgcag
     3961 atgatgcaaa ccacaaacca gccccagcga cagggtattt tcattggtat aaaggaagcc
     4021 accgcccatc aggccatcgg tgggtgatcc cgcaaacagg caagccgcgc cctgattacc
     4081 ctgcaactga aaacggtctt cgataaccga cttcggtaac tcgatcagtt ccttcacgcc
     4141 aaccgccaca tccgtcggtt taacgcgttt tgccatcccc agcttttcgg caaggatgga
     4201 attcacccca tcagcaagga tcaccgtttt cgcttcaatc acatcgccat cagcttctac
     4261 gccgacaact ttgccatcgc gctgtacgag gttatcgacg cggatcccgg taattaactg
     4321 cgcgtccgct tcttcggcct gctccatcag ccaggcatca aatttactgc gcaaaacgga
     4381 gtaagaacgc tgggatggcg aggtttcgtc gccattgcag tagtccatag tcatcgctga
     4441 cttttccgtc ataaacgcga gtttttcatg ggtgatcagg cgttctacgg gggcggagtc
     4501 agcgaaacca ggaataatgt gttccagact gtgggcatag agacgcccgc cggtgacgtt
     4561 cttggcacct gcggaattgc ctcgctcgat aactaacact tgcgcacctt cgcgggcgag
     4621 caccagtgcg gcaaccgaac cggcaagccc tgcaccgacg atgatggcgt caaagatatc
     4681 ttcggacata agagctccct gtcagcggca gggccaaaat gcagccctgc cagagtggat
     4741 caacgcgcta aagctgcggt cagtgccgga aggattttca cggcgtcgcc aacaatgccg
     4801 taatccgcgt actggaagat cggcgcattt ttatctttgt tgatggcgaa aatggtttgc
     4861 gacgcattag cgccaaccat gtgctggatc tgcccggaga tccccaccgc caggtacagt
     4921 tcaggtttca gcatcaggtt ggagataccg acatagcgtt cgtgctccat ccatttttcg
     4981 ttttccgcca ccggacgaga acaggccagc tccgcaccta tcgccttaca aagctgttct
     5041 gccagcgcaa tgttctcttt gctgccaata ccgcgaccga cgctgaccac cagtcgggct
     5101 ttgtcgagat cgacgctgtt gctctgacgc gcctgggttg ccgtgcgggt aatcgccaca
     5161 gccggagcct gccactccac ggtgtgcgtt tcgccagtgc gtgacgcgtc tggctgagcc
     5221 gcatcgaacg tgccgctgct gatggtcagt accgcatacg gcgtggcaat gcgttcttcg
     5281 ccaatcgcca gaccgccgta aaccatgtgt ttcactgtcg ctttaccgtc ctgtacgctg
     5341 acggtgctgg catcgttaga caccgccgct ttaaggcgat aacccagttt tgccgccagt
     5401 aatttgccgc gacgggtgtt tggcaacagc accagaccgt ctgcgccgtg ctggcgaata
     5461 gtgtcagcca tgacaccggc gtaatcttcg atcatccgct cgtccggttt gccgcttaat
     5521 ttccagacat gattagcgcc gagctggatt gcctgtgcgc cgtcggcatc attgaggaca
     5581 aaagtgttga tttgattagc taaagcctgc gcaccgttca tcagttccgg cagacgagaa
     5641 ggggtatcgc tgaataccca gacttgagaa aacgtgttca tagcatcccc tgtaattaaa
     5701 tgactttgcg aagattttca gcaaatgcgg cgatctgttc ttcgccgtcg ccttcaatca
     5761 cgatgcgctg acgctcgcgc tgtttcggcg cggcaacctg ttgttctgac caggctgcct
     5821 ctgcgttaaa accgatatcc gccgccgacc atacctggac tggctttttc gccgcgccga
     5881 gaatggcttt catcgaagga atttgtgggg agttgatatc agtggaaaca gcaacaaccg
     5941 caggcagcgg aatgcttaag gtttcggttt catcttccag ttcgcgctca acggtgaggg
     6001 tatctgccgt cagggagata attttgctga cgccgttaac tgccggaata ttgaggattt
     6061 cgcctaccag caaaccaacc tgctgggcat aaaggtcgga agaaccatcg ccacagagga
     6121 tcaaatcaaa gcctgctttc tgggcggcgg cagccagtgc gctcgccgtt tgttgcggca
     6181 gtgcctgctc gaactggtca tcaatcacca caatcagttc atccggaccg cgcgatagca
     6241 catctttacg ccctttggcg ttggtcaggg cttttccgcc cacacttaag gccgtcacct
     6301 gcccctctgc tgcctgttgc tttagctggc aagccgcttc aatagcgttg agatcgtatt
     6361 ggcttatttt ggcatcggct ttactgaagt ctaacgaacc atcagcatta ttgaccgcaa
     6421 tatcctgttc atcaggcacg cacttatagc aagtaataat cttcattgca tctccagaaa
     6481 tcatgaagga aacgtcgttc atatcggtgg tgttaattac agatatcacg ccctttaatt
     6541 cggaaataaa actttaatca ccaatattga aaatgtcaca cgcagataaa tccaactttc
     6601 aatattgtta agctcctcac caatattgaa aacatggcgt agcaaaaaga aattttcaat
     6661 attgttttat ggatcaccaa tattgaaagt cagatgctat ttttttactc acaatcaaac
     6721 tcatcacatt gataaacaat gaatatttac acaaaaacgc cctgttttta cccgataaaa
     6781 aactgtgact aacatcacag aatgcagcca attgaatacc cattatgagt tagccattaa
     6841 cgcgtccacg aggttaataa taattatatt aaatgttaac aaaaataaaa caaacgggaa
     6901 cgcaaaataa atattcgttt tcacagtgga attaactcat gaagaatgaa aagagaaaaa
     6961 cgggaataga accgaaggtt ttctttccgc cattaataat cgtcggcata ctttgttggc
     7021 ttacagtcag agatctggat gcggcgaatg tcgttattaa tgctgtattc agttacgtca
     7081 ccaatgtatg gggatgggca tttgaatggt atatggtagt gatgcttttc ggttggttct
     7141 ggctggtgtt tggcccgtat gccaaaaagc gtttaggtaa cgaaccacct gaatttagta
     7201 ccgccagttg gatctttatg atgttcgcct cctgtacgtc tgctgccgta ctgttctggg
     7261 gatcgattga gatctactac tacatctcca ccccgccgtt tggcttagaa ccgaactcga
     7321 caggggcgaa agagttgggg ctggcttaca gcttgttcca ctggggacct ctgccgtggg
     7381 ccacttacag cttcctttct gtcgccttcg cttacttctt ctttgtccgc aaaatggaag
     7441 tgattcgccc cagctccacc ctggtgccgc tggtgggtga aaaacacgcc aaagggttgt
     7501 tcggtaccat cgtcgacaac ttctatctcg tcgccttgat tttcgcgatg ggtaccagtc
     7561 tgggccttgc cacgccgctg gtgaccgagt gtatgcaatg gttgtttggc attccgcata
     7621 ccctgcaact ggacgctatc atcattacct gctggattat cctcaacgcc atttgcgtcg
     7681 cctgcggtct gcaaaaaggg gtacgtatcg ccagtgacgt gcgtagttac ctgagcttcc
     7741 tgatgctggg ttgggtgttc attgtcagcg gtgccagctt catcatgaac tacttcaccg
     7801 attcggtggg gatgttgctg atgtatctgc cgcgcatgtt gttctatacc gatcccatcg
     7861 ctaaaggcgg cttcccgcag ggctggaccg tgttctactg ggcatggtgg gtgatttacg
     7921 ctatccagat gagtatcttc ctcgcccgca tctcccgtgg tcgtaccgtg cgtgaactgt
     7981 gcttcggcat ggtgctgggg ctgacagcgt caacctggat cctgtggact gtactcggta
     8041 gtaacactct gctgttgatg gataaaaaca tcatcaacat tccaaatctg atcgaacagt
     8101 acggtgtggc gcgcgccatc atcgaaacct gggccgctct gccactcagc accgccacca
     8161 tgtggggctt cttcatcctc tgctttattg ccaccgttac gctggttaac gcctgctctt
     8221 ataccctggc gatgtccact tgccgcgaag tacgcgatgg tgaagaacca ccgctgctgg
     8281 tgcgtatcgg ctggtcaatt ctggttggca ttatcggtat tgttctgctg gcgctcggcg
     8341 gcctgaaacc gattcaaacc gccattatcg ccggaggatg cccgctgttc ttcgtcaaca
     8401 ttatggtgac gctctccttt attaaagacg cgaaacagaa ctggaaagat taattaaccc
     8461 ccaaaatatc aagaggttga aagatggatt ttaatttaaa tgatgagcag gaactgtttg
     8521 tcgccggtat ccgcgaactg atggccagcg aaaactggga ggcctatttt gccgagtgcg
     8581 accgtgacag cgtctacccg gaacgttttg tcaaagcact ggcggatatg ggtatcgaca
     8641 gtctgctgat ccctgaagag cacggtggtc tggacgcggg gtttgttact ctcgccgccg
     8701 tgtggatgga gctgggacgt ctgggggcac caacctatgt gctgtaccag ttgccgggcg
     8761 ggttcaatac cttcctgcgc gaaggcacac aagagcagat cgacaagatt atggctttcc
     8821 gcggcaccgg taagcagatg tggaactcag cgattaccga accgggtgcg ggctccgacg
     8881 tgggtagcct gaaaacgact tatacccgta gaaatggtaa gatttatctt aatggtagta
     8941 agtgttttat taccagtagc gcctacaccc cgtacatcgt ggtgatggcg cgcgacgggg
     9001 cttctccgga caaacctgtc tacaccgaat ggtttgttga tatgagcaaa ccgggcatca
     9061 aagtgaccaa acttgaaaag ctcggtctgc gtatggatag ctgctgtgaa atcacttttg
     9121 acgacgtgga actggacgag aaagacatgt tcggtcggga aggtaacggc tttaaccgcg
     9181 tcaaagaaga gttcgaccat gaacgtttcc tggtagccct gaccaactac ggtacggcga
     9241 tgtgcgcctt tgaagatgcg gcgcgctacg ccaaccagcg tgtgcagttt ggcgaggcta
     9301 ttggtcgttt ccagttgatt caggaaaaat tcgcccacat ggcgatcaaa ttaaactcca
     9361 tgaaaaacat gctgtatgaa gcagcgtgga aagcagacaa cggcaccatc acctctggcg
     9421 atgcagcgat gtgcaaatac ttctgcgcca atgcggcatt tgaagtggtg gatagcgcaa
     9481 tgcaggtgct gggcggtgtc gggattgcgg gcaaccaccg catcagccgc ttctggcgtg
     9541 acctgcgtgt agaccgcgtt tccggaggat ctgacgaaat gcagatcctg acgctgggtc
     9601 gcgcggtgct gaagcaatac cgctaagtcc gtgcgctgcc tgatgcgacg ctgacgcgtc
     9661 ttatcagacc tacgggagca cgaatgtagg ccggataaag cgtttacgcc gcatccggca
     9721 gtcattcgct acataaattt ttcaggagct aataatggat catctaccca tgccgaaatt
     9781 cgggccgttg gccggattgc gcgttgtctt ctccggtatc gaaatcgccg gaccgtttgc
     9841 cgggcaaatg ttcgcagaat ggggtgcgga agttatctgg atcgagaacg tcgcctgggc
     9901 cgacaccatt cgcgttcaac cgaactaccc gcaactctcc cgccgcaatt tgcacgcgct
     9961 gtcgttaaat attttcaaag atgaaggccg cgaagcgttt ctgaaattaa tggaaaccac
    10021 cgatatcttc atcgaagcca gtaaaggtcc ggcctttgcc cgtcgtggca ttaccgatga
    10081 agtactgtgg cagcacaacc cgaaactggt tatcgctcac ctgtccggtt ttggtcagta
    10141 cggcaccgag gagtacacca atcttccggc ctataacact atcgcccagg cctttagtgg
    10201 ttacctgatt cagaacggtg atgttgacca gccaatgcct gccttcccgt ataccgccga
    10261 ttacttttct ggcctgactg ccaccacggc ggcgctggca gcactgcata aagtgcgtga
    10321 aaccggcaaa ggcgaaagca tcgacatcgc catgtatgaa gtgatgttgc gcatgggcca
    10381 gtacttcatg atggattact tcaacggcgg cgaaatgtgc ccgcgcatga ccaaaggtaa
    10441 agatccctac tacgccggtt gcggtctgta taaatgcgcc gacggctaca tcgtgatgga
    10501 actggtgggc attactcaaa ttgcagagtg ctttaaagat attggcctcg cgcatctgct
    10561 cggcacgcca gaaattccgg aaggcactca acttatacac cgtatcgaat gcccttacgg
    10621 cccactggta gaagagaaac tcgatgcctg gctggcggca cataccatcg cagaagttaa
    10681 agaacgcttt gccgaactga atatcgcctg tgccaaagta ctgaccgtac cggaactgga
    10741 aagcaatcca cagtatgtgg ctcgcgaatc aatcactcag tggcaaacga tggatggtcg
    10801 cacctgcaaa gggccgaaca tcatgccgaa attcaaaaat aaccccggac aaatctggcg
    10861 cggaatgccc tcacatggca tggacacggc tgccattttg aaaaatatcg gctacagcga
    10921 aaacgacatt caggagttgg tcagcaaagg tctggccaaa gttgaggact aaacgcgtgg
    10981 cgtcagtcag cttgcctgac tagcgcccgc ccaataacaa acgaacaaaa atggatagag
    11041 gtgcaatgga tatcattggc ggacaacatc tacgtcaaat gtgggacgat ctggcggacg
    11101 tttacggtca taaaacggcg ctgatttgtg aatccagcgg cggagtcgtt aaccggtata
    11161 gttatcttga gttaaatcag gagattaacc gcacggcaaa cctgttttat acgctgggga
    11221 ttcgcaaagg cgacaaggtt gctctacatc tcgacaactg cccggaattt atcttttgct
    11281 ggttcgggct ggcaaaaatt ggcgcgatta tggtgccgat taacgcccgc ctgttacgcg
    11341 aggaaagcgc gtggatcctg caaaatagcc aggcgtgcct gctggtgacc agtgcgcaat
    11401 tctatcccat gtatcaacag attcagcagg aagatgccac gcagttgcgg cacatttgcc
    11461 tgacagatgt ggcacttccc gctgatgatg gcgtgagttc gtttactcaa ctgaaaaatc
    11521 aacaacctgc caccttgtgc tatgcaccgc cgctatcgac tgacgatacg gcggaaattc
    11581 tcttcacctc cggcactacc tcccgaccga aaggtgtggt gattacccat tacaacctgc
    11641 gcttcgctgg atattactcc gcctggcagt gtgcactgcg tgacgatgac gtctacctga
    11701 cggtaatgcc tgcgtttcat atcgattgcc agtgtactgc ggcgatggcg gcgttttctg
    11761 ccggggccac ctttgtgctg gtcgagaaat acagcgcccg cgccttctgg ggacaggtgc
    11821 agaagtaccg cgccaccatt accgaatgta ttccgatgat gatccgtacc ttgatggtgc
    11881 aaccgccttc tgcgaacgat cagcaacacc gcctgcggga agtgatgttt tatctcaact
    11941 tgtcggcgca ggaaaaagac gcattttgtg aacgcttcgg cgttcgcttg ctgacgtctt
    12001 atgggatgac ggaaaccatt gtgggcatta tcggcgatcg ccctggcgat aaacggcgct
    12061 ggccgtcgat tggtcgggcg ggcttttgct acgaagcgga gatccgcgac gatcacaatc
    12121 gcccgctccc ggcaggtgag atcggtgaaa tctgtattaa aggcgtacca gggaaaacca
    12181 tcttcaaaga gtattttctc aacccgaaag ccactgcgaa agtgctggaa gccgacggct
    12241 ggctacatac cggcgatacc ggataccgcg acgaagaggg ctttttttat ttcgtcgatc
    12301 gccgctgcaa tatgatcaaa cgcggtggcg agaatgtctc ctgcgtggag ctcgaaaata
    12361 tcatcgccac ccatccaaaa attcaggata tcgtggttgt gggaattaaa gattcgatac
    12421 gcgatgaagc catcaaagca tttgtggtgc tgaatgaagg tgaaacattg agcgaagagg
    12481 aatttttccg cttctgcgaa caaaatatgg cgaaatttaa agtgccctct tatctggaga
    12541 tcagaaaaga tctgccacgt aattgctcgg ggaaaataat tagaaagaat ctgaaataaa
    12601 tgaaacggca gggaactacc ctgccagcta ataatcacgc tctaaaacaa tatgcatttt
    12661 ttgcagggat gttgtcgtcc ctgaaaaagc aaaaatggag aaaaggaatg agtgaatcat
    12721 tacatctgac ccgcaatgga tcaattctgg aaattaccct tgatcgtcca aaagcgaatg
    12781 ctattgatgc aaaaaccagc tttgaaatgg gcgaagtatt tctaaatttc cgtgacgatc
    12841 cgcaattacg tgtcgccatt attaccggtg ccggagagaa attcttttcc gcgggctggg
    12901 atttaaaagc ggcagcagaa ggcgaagcac cggatgctga ctttggcccg ggaggttttg
    12961 cgggattaac cgaaattttc aatctcgaca aaccggtcat cgcagctgtg aacggctatg
    13021 cctttggcgg cggctttgaa ctggcgctgg cggcagattt tattgtttgt gccgataacg
    13081 ccagcttcgc cctaccggaa gccaaactgg gcatcgttcc tgacagcggc ggcgtgctgc
    13141 gtctgccgaa gatcctgccg cctgccatcg tcaatgaaat ggtgatgacc ggcagacgaa
    13201 tgggcgcaga agaggcgctg cgttgggggg tagtcaaccg cgtggttagc caggcggaac
    13261 tgatggataa cgcccgcgaa ctggcgcagc agctggttaa cagcgctccg ctggcgattg
    13321 cggcgctgaa agagatctac cgcaccacca gcgaaatgcc ggtagaagaa gcgtatcgct
    13381 atattcgcag cggcgtgttg aaaaactatc catcggttct gcattcggag gatgccattg
    13441 aagggccgct ggcgtttgcc gagaagcgcg atccagtgtg gaaaggacgt taacaaccgt
    13501 gagctattac gcctttgagg ggttaattcc ggtggttcac ccgacggcgt ttgtccatcc
    13561 cagtgcagtc ttgattggcg atgtgattgt gggagccggt gtctacatcg gtccactcgc
    13621 ctcactgcgt ggtgactacg ggcggttgct cgtgcaagcg ggagccaata ttcaggatgg
    13681 ctgcattatg catggctact gcgacactga caccatcgtt ggggaaaacg gccatatcgg
    13741 gcacggagcg atcctgcatg gttgtgtgat tggtcgcgat gcactggtcg ggatgaacag
    13801 cgtgattatg gatggcgcgg tcattggcga agagagcatt gttgccgcta tgagctttgt
    13861 caaagcgggc tttcgcggcg agaaacgcca gttgttgatg ggtacgccag cccgcgctgt
    13921 acgcagcgtg agtgacgacg agttacactg gaaacggttg aatactaaag agtatcagga
    13981 tcttgtcggg cgctgtcatg catcgttaca tgaaacgcaa ccactgaggc aaatggaggg
    14041 aaatcgcccc cgtttgcagg ggacgacgga tgttgcgccg aaacggtaac atcgtcggat
    14101 gcggcataaa cgccttatcc gacctggctg gagagcaacc gtaggccgga taagatgcgc
    14161 cagcatcgca tccggcgacc aggtcgcacg gtatcatcga aatgacatta acgacgcata
    14221 ctctttgaca acatcatctg ccacttctgc tcgcggttaa gttccgtgag cggaacggca
    14281 ggaatacgcg gcgtctttct tacgccgact aatttttccc gactgttatt gcgcaggcgc
    14341 gcgtaaattt gctcatcgat atcgaccact ttaaccagtc gctgacactg gcatccccgc
    14401 ccttccagct tattagggat catcttaact tcgcagctta tttctgtgac ttccgacaga
    14461 atataagtca gggtattaac cgccttgctg tgttcaatat cgaaatgaat agagatctct
    14521 cttgctatca cccaccgatt ttcagccatc atccattcgg cgattaacaa gtagagtggt
    14581 ttttcaacat atccttcaca catacgaact ccagtttcac aaattaaaag tctggctatg
    14641 actcgttttg aagcaattaa acaaggccat attaaaattg tggatatatc gatagtatgc
    14701 aacttcaccg tcgataagtg tgagctaaat cctgcttatg tcatcaaaaa tatcgattcc
    14761 ccaaaagatt tacttaatgg gcaaaaaaaa cggtcctcat cagagaaccg tattagttat
    14821 tcgatcaaat tagcggatga aaaatatccg ccatgacacg ctattatttg atctgcgcgt
    14881 gcatttcctg caccgaaatt actttttcag tcgcatcggc attcagcgcc atcgcggtag
    14941 cgaaaccacc gttcagggtg gtgtcataat gcactttata ttgcagcgca ctgcgacgga
    15001 tcacgcggga gtcttcaatc gcacgacggc ctgaggtggt gttgatgatg taggtatatt
    15061 cgccattctt gatgcggtcc tgaatgtgcg gacggccctc atgcaccttg tttaccagac
    15121 gcggattgat acccgcttcg cccagcacaa tcgccgtgcc gtgggtcgca tccagctcga
    15181 agccctgttt cagcagtttt gccgccaggt ccaccacgcg ttctttgtcg ccttcgcgca
    15241 cggaaagcag cgcacgaccg tgtttcttca tggtggagtt gctgcccagc tgcgctttgg
    15301 caaacgcttc agcgaaggtg cggcccacgc ccatgacttc cccggtagag cgcatttctg
    15361 gccctaacag cgggtcaacg cccgggaatt tattgaacgg cagcaccact tctttcaccg
    15421 agtagtacgg cgggataact tctttggtta cgccctgctc ggccagcgtt ttgcctgcca
    15481 tcacgcgcgc cgccactttc gccagcggta cgccggtggc tttggagacg aacggaacgg
    15541 tacgcgccgc acgcgggtta acttcaatca ggtagacttc gttgtttttc accgcaaact
    15601 gcacgttcat caggccgcgc acctgcaatt cgaaggccag tttctgcacc tgctggcgca
    15661 tcacatcctg aatttcctga cttaaggtgt aggctggcag agaacatgcg gagtcaccgg
    15721 agtgcacgcc cgcctgctca atatgctcca tgatgccgcc aatcagcacc atttcgccgt
    15781 cgcagatggc atccacgtca acttctactg cgtcatcaag gaagtggtcc agcaacactg
    15841 gcgcatcgtt agatacgctg accgctgtct ggaagtagcg acgcaggtca gcttcgtcat
    15901 agacgatttc catcgcccga ccgccgagaa cgtaagacgg acgcaccacc agcgggtagc
    15961 caatctcttt cgctttctca accgccattt cgatggtggt aacggtggcg ttcgccggtt
    16021 gtttcagttt cagacgctca accgcatgct ggaagcgttc acggtcttcc gcacggtcga
    16081 tggcatccgg gctggtaccg ataaccggta cgccagccgc ttccagcgcg cgcgccagtt
    16141 tcagcggggt ctgaccgccg tactggacga taacgccttt cggcttctca atacgcacaa
    16201 tttccagcac gtcttccaga gttaccggct cgaagtagag gcgatcggaa gtgtcgtagt
    16261 cggtagagac ggtttccggg ttacagttaa ccataatggt ttcgtaaccg tcttcgcgca
    16321 gcgccagcga ggcgtgtacg cagcagtagt cgaattcgat accctgaccg atacggttcg
    16381 ggccgccgcc gagcaccatg attttttcac ggtcggtaga cggattcgct tcgcactctt
    16441 cttcataagt ggagtacatg taagcggtgt cggtggcgaa ctctgccgca caggtatcca
    16501 cgcgcttata aaccgggtgc aggtcatact ggtcgcgcag cttgcggatt tctgcttcac
    16561 gcacgcccgc gagttttgcc agacgcgcgt cggcaaagcc tttgcgtttc agctggcgca
    16621 ggaagtcagc gttcaggcca gtgatgccca cttctgcgac tttctcttcc agacgcacca
    16681 gctcttcaat ttgtaccagg aaccagcggt caatgttggt caggttgaac acgccgtcta
    16741 cggacaggcc cgcgcggaaa gcatcggcga tataccagat acgctctgcg cctgcatctt
    16801 tcagttcgcg gcgaattttg gtcagcgctt ccggatcatc gaggctgact ttcgggtcga
    16861 agccagttgc gcccacttcc aggccgcgca gcgctttttg cagggattcc tgctgcgtgc
    16921 gcccaatcgc catcacttcg ccaaccgatt tcatctgagt ggtcagacgg tcgttcgccc
    16981 cggcgaattt ctcgaagttg aagcgaggaa ttttggtgac cacgtagtcg atggacggct
    17041 cgaaggaggc cggagtacgc ccgccggtga tgtcgttcat cagttcgtcg agggtgtaac
    17101 ccaccgccag tttcgccgcc actttagcaa tcgggaaacc agtggctttc gacgccagcg
    17161 ccgaagaacg cgacacgcgc gggttcattt cgataacaat caggcgaccg tttttcgggt
    17221 tcaccgcaaa ctggacgtta gaaccaccgg tttcaacgcc gatttcacgc agcaccgcca
    17281 tcgaggcgtt acgcatgatt tgatattctt tgtcggtcag cgtttgggct ggcgcgacag
    17341 tgatggagtc accggtgtgg atgcccatcg catcaaagtt ttcgatagag cagacgatga
    17401 tgcagttgtc gtttttatca cgcaccactt ccatctcgta ctctttccag ccgatcagcg
    17461 actcgtcaat cagcaactct ttggtcggag agagatccag accgcgggcg caaatttctt
    17521 caaactcttc acggttataa gcgataccgc caccgctacc gcccatggta aaggatgggc
    17581 gaataatgca cgggaagcca acttcagcgg caaccgctag cgcttcttcc atcgtgtgtg
    17641 cgataccgga acgcgcggtt tccagaccaa ttttcttcat cgctacgtcg aaacggcggc
    17701 ggtcttcggc tttatcaatc gcatcggcag tggcaccaat catggtgacg ccgaactctt
    17761 ccagcacgcc ctgacgctca agctccagcg cacagttcag cgccgtctgc ccccccatgg
    17821 ttggcagcac cgcgtccggg cgctcttttt caataatttt gcgtaccact tcccagtgaa
    17881 tcggctcgat gtaggttgca tcagccattt ccgggtcggt catgatggtc gccgggttgg
    17941 agttcaccag aatgacgcgg tagccctctt cacgcagggc tttacacgct tgcgcgccag
    18001 agtagtcaaa ctcacacgcc tgaccgataa caatcgggcc cgcacccaga atcaggatac
    18061 tttttatatc tgtacgtttt ggcatggctc ttttactcct gattacttag cggttttacg
    18121 gtactgctca attaactcga taaagtggtc gaacaacggc gcggcgtcgt gtggaccagg
    18181 gctggcttcc gggtgaccct ggaagctgaa cgccggttta tcggtgcgat gaatgccctg
    18241 taacgtaccg tcgaacaggg atttatgcgt gacacgcagg tttgcaggta atgttgcttc
    18301 atccaccgca aaaccgtggt tctgggcggt gatcatcacc acgtttttct caacatcttt
    18361 aaccggatgg ttgccgccgt ggtgaccaaa tttcattttg acagtcttcg caccgctcgc
    18421 cagcgccagc agctgatgac cgaggcagat gccgaatacc ggaatatcgg tttcgaggaa
    18481 tttctggatg gcggtaatgg cgtaatcgca tggggccggg tcgccagggc cgttggagag
    18541 gaagatgccg tctggattca ttttcaacac atcttccgca gaagtttgcg ccggaacgat
    18601 agtcagacga cagcctctgt ccaccaacat gcgcaggatg ttgcgcttgg caccaaaatc
    18661 ataagccaca acatggaacg gcagctcgtc ttctttcttc gcttctggca ggccgccggt
    18721 cagcgtccag ctcccttgtg tccagctata cgtttctgcg gtggtcactt cttttgccag
    18781 atccatgccg ttcagacctg ggaacgcgcg ggctttttct aacgccagcg ccgcatccgg
    18841 gttatcgccc gcgataatgc agccattctg tgcgcctttc tcgcgcagta aacgcgtcag
    18901 cttacgggta tcgatatcgg caatcgccac gatgttatgg cgcttcaggt aagaagagag
    18961 gtcttcggta ttacggaagt tgctggcaat cagcggcagg tcgcgaatca ccagaccttg
    19021 tgcatgtacc tgagaagatt cttcatcggc gtcattagtg ccgacattgc caatatgggg
    19081 ataagtaaga gtaacgattt gacgagaata ggaaggatca gtgaggattt cttgataacc
    19141 ggtcattgaa gtattgaaaa cgacttcccc aaccgccgaa cctgttgccc ctatggcccg
    19201 accgtgaaac tgggttccgt cttccagaac caatagcgct gacttaatca aaacaccctc
    19261 cagagaatat tcactcactt tatttgcata ttaattcaca atgatgacgt aaatcaatgc
    19321 aaatctgctt accgacgaat ttctggcaaa cggcggcatt ctggagatat gggagctaaa
    19381 agtcaactta atggcgggat tttttatatt attttgtggc tcatgcatgt tttacaggca
    19441 ttaagcgaca aaaagatcat ataacatgtt ctggaaaacg cttgcgcagc aagaagattg
    19501 catgaaatca aaatgagcag aaaaaagtgg accaaatggt caaaatttac attaaaacaa
    19561 caaaaacaga atgcaaaaca tgtaattttt ggcatttaca ttaaatttaa aagcataaca
    19621 ctaaaaataa aagggccaaa aattaaagcc ctttatattc aatggattaa atgtgttatt
    19681 tttgcatcac tataaatatt ttgtggttac agattattga gatcaagcac atctcgcata
    19741 tcaaaaagac cactttcctt accactcaac cacaaagccg atcttaccgc accgttagca
    19801 aatgtcatgc ggctggatgc cttatgggtg atctccagac gctcgccaat atcggcaaac
    19861 atcgcggtat gttcgccaac gatgtcacct gcacgcacgg tggcaaaacc aatggtgcca
    19921 ggcacacgtt caccggtatg gccttcacga ctgtagaccg cgcaatcttt cagatcttta
    19981 tcaagagcgt gggcgatcgc ctctcccatt gccagtgcgg tgcctgacgg cgcatcaact
    20041 ttatgtctat gatgtgcttc aataatttcg atatcggtgt agtcccccat cactttggct
    20101 gctttctcca gcagtttaag catgacgtta acgccaacgc taaaattggc ggcaaagaca
    20161 atcgcaatat cggcggcggc gtcacgaatc gcctgtttac cggcttcgtc aaaccccgta
    20221 gtgccgatca ccatcccttt gccatgctgg cgacaaaaag cgagatggtt cagcgtacct
    20281 tccggacggg taaaatcgat aaacacatca aaatcatctt ttatcgcatc aaggctgctt
    20341 tgcacagtaa cgcctgtttt tcctgcaccc gccagctcac cggcgtcgct gcccagtaaa
    20401 gaagatcctt cgcgctccag cgccgcgccc agctgcacgc cttctaatgc cagcgccgcc
    20461 tgaatcaact ggcggcccat acgccccccg gctcccgcga tggcaacgcg gatgtttgca
    20521 tcatgcatag ctattctctt ttgttaattt gcatagaccg ttttcagagt aaccagcgca
    20581 tcgctacgcc gccagccaaa aacgctgata attagaaatt aatgataaac agggagggat
    20641 atcagtgaaa ggcatgagtc gatgaatgac tgcatgccga taacatgtga caggttacga
    20701 cgccagagcc agcacctcag ccacccactg ctggaaacct ttcacatcca gatccagcgc
    20761 cacctgcacg tttgcaggtt tactcaggca accatcaata tcaacaaccg tagtacctga
    20821 ggtaaattca ccctgagttt ccaccgccac aaaacacggt ttgagagtga acaagtctgg
    20881 gcgcaccagc caggcgatag cgcagagatc gtgcatacgc agaccgcttt gcatactgcc
    20941 gctacggtag tggctaaaca gggcgtgcaa cattttcccg gtacgattca actccggcaa
    21001 agtagcgaga tagtcaggag ttaatattgc ctgattggtg acatccagac cgcacataac
    21061 gatttcgatg ccgctacgga agacacaggc tgccgcttct ggatcggcag cgatattaaa
    21121 ctcggcgttt ggtgtacagt tgccgcgacc agcagaaccg cccatgatca ccagacgacg
    21181 aatatactgt ttacattccg ggcattgtga aagtaacagc gcaatattgg ttaacgggcc
    21241 gatggccacc agggtaacag gctctggtgc acgcatcagg gcatcacgaa tcgccagaaa
    21301 tgcgggtttg tccagtgggc ttctgttgtg ctcgacaaag tcgtagccag ccattcccga
    21361 ttcaccgtgc acagatgctg cattacgcgg tgcgcgtacc agcggcacag cggctccctg
    21421 ggcgagcgga atctccgcat tccagaaatg cagcagttgc agggcgttgc gggtagtttt
    21481 ctctaccgag acattacccg cgacggtggt catcagttgc aggtcaagtt ccggggcaaa
    21541 aatcgcggcg gcaatggcga cggcatcgtc aatgccgggg tcagtatcga ggaagatagg
    21601 taaacgcatg ttttctccat aaaaaatgcc ggtaacaaga ccggcatttt cgcataactt
    21661 aggctgctaa tgacttaatc gacttcacga atatcgacac gcagctcttt cggcacttcg
    21721 aaaacaatgt tttcttcacg gccttccagc ggaatggctt caccaccacc cagctgctgc
    21781 aaacgtgcca ccacattctg caccagaata tccggagccg atgcgcccgc agtcacgccg
    21841 acgcatttaa cctctttcac ccactcttcc tggatatctt tcgcatcgtc aatcaaaaac
    21901 gcgcgtttgc ccatacgttg ggccagctcc gccagacggt tggagttgga ggagtttttc
    21961 gaaccgacca ccagcacaac ttccgcctgt tctgccaggg cgcgtaccgc ttcctgacgg
    22021 ttagtcgtgg cgtaacagat gtcatctttg cgcggaccga caattttcgg gaagcgttta
    22081 cgcagcgcgt cgatcacatc agacgtgtca tccaccgaca gcgtggtttg ggtcataaag
    22141 gagagcttct cttcgttttt gaccgtcagt ttccacacat cgtctggcga ttcgaccaga
    22201 tacattcccc cttccgggtt gctgtactga cccattgtcc cttccacttc cgggtgaccg
    22261 gcgtggccga tgagaataga ttcttcgcca cggcgactgg cgcgggcgac ttccatatgc
    22321 actttggtca ccagcggaca ggtggcgtcg aataccgtca aatcacggct tttcgcttcg
    22381 ttacgtaccg cctgagaaac accgtgtgcg gagaaaatca ggatcgcgcc gtccggtact
    22441 tcgctaatct gctcaataaa gatagcccca cgctcgcgca ggctatcgac cacgtagcgg
    22501 ttatgcacca cttcgtgacg gacatatatc ggtgcgccgt aaatggccag cgcgttttca
    22561 acaatgctga tagcgcggtc taccccggca caaaaaccac gcgggttggc caacaggatc
    22621 tgcatgttac gcctccagtg ccggatcgat ttccagcact tcaatatcaa aatgaacggt
    22681 ctgcccggcc agcggatggt tgaaatcaac ggtaatggag tcgccgttaa tttcgcggat
    22741 cacgccaggc atctcactgc catccattgc ggtaaaaagc atgattgcgc caatttctgg
    22801 ctcgcctgca tccataaact cacggcggga gaagtactga atcaggtctg gtgacggcac
    22861 gccaaacgcc gcatcgggct ccagcgagaa agtggtttta tcgcccactt tcagccccag
    22921 caggtgttgc tccagccctt cagaaagaga agcatcgccc aggcggaaca gtgccggttt
    22981 accattgttg cgggtagact cggcggtggt gccatcgtcg agttttagcg tgaagtgcac
    23041 caggacggcg ctattgctct gtacagattc agacatgcag gtttgctctt atattttgag
    23101 gatttttgcc ggatgcagcc tacgatttcg cagcaatctg taggctgcat ccggcagggt
    23161 ttattattgt tttttcgctt tagaaggcaa aaaaccttcc agcacaatca gtgccgcacc
    23221 gacacagatg gcagtatcgg caaggttgaa ggtagcgaag tgccagtcgc cgacgtagaa
    23281 gtcgatcata tcgacaacga agccgtgcca caggcggtcg aacaggttgc ccagcgcacc
    23341 gccaataatc agcgcgtaag cgatattgtt tagcttctgc gtggccttcg agcgatacat
    23401 catcactgcc aggatcacgc taataccaat cgcaataccg gcaaagaacc aacgctgcca
    23461 gccgccgcta tcggcaagga aactaaacgc cgcgccatag ttacgcgcat aatgcagatt
    23521 aagcgacggg aacagcggga ccgtatcccc cagagcaaag ttctggagga tcaggtattt
    23581 gctgcccaga tcgataatca gcacgactac caccagccac agccagcgta gccctgttga
    23641 acagatcgat tgactcatca ggcaaactta cgtttttcac cgtcaccggc gacgttgctg
    23701 acacagcggc cgcagatttc tgcgtgttcc gccaccttgc cgacatcctg ggtgtagtgc
    23761 cagcagcgtg ggcacttctc accttcggct ttactcaacg cgactttcag ccctttgagt
    23821 acttcgctct gctgagcatc agcaggtgca tcgttatagt ctgcaacggt agcgccggag
    23881 gtcaacagga caaatcgtaa ttcatcgccc agcgcggtca gtttcgccgc cagttccggt
    23941 tctgcataca aggttactgc cgcttccagc gagccgccca ctttcttgtc ggcacgcgct
    24001 tgctcaatga ctttgttcac ttcgccacgc actttcaaca gctcgtccca gaacgcatcg
    24061 ttcatcgctt cactgtctgc cagaccaaac aggccttcgt accactcgcc ggtgaagacg
    24121 tatttttcac gttcgcccgg caggtagccc cacacttcat cagcggtgaa ggagaggatt
    24181 ggtgccatcc agcgcaccag cgcttctgcg atgtgataca gcgcagtctg gcagctacga
    24241 cgcgccacac tgtcagcttt ggcggtatac tgacggtctt tgatgatgtc gaggtagaat
    24301 gaacccatct caacggagca gaagcgcatc agacgctgta ccacttcgtg gaaatcgtat
    24361 gcttcgtacg ccttgaggat gtcttcctgt gccgctttcg cacaacctac ggcccagcga
    24421 tccagtacca ccatctcttc cggtttcacc atatcttttg ccggatcaaa accgttcagg
    24481 tttgccagca ggaagcgcgc ggtgttacgg atacgacgat agctgtcggc agcacgtttc
    24541 aggatttcgt cagaaacggc catttcacca gtgtagtcag ttgatgccac ccacagacgc
    24601 agaatatccg cgcccagttt gttcatcaca tcctgcggcg aaacggtgtt gccgatggat
    24661 ttagacatct tgcggccctg accatccacg gtaaagccgt gggtcagtac ctgacgatac
    24721 ggtgctttgc ccttcatcgc ggtagagatc atcagagagg acatgaacca gccacggtgc
    24781 tggtctgaac cttccagata catgtccgca gcgtgaccgg caaattccgg acgcacgtca
    24841 acaacggaag agtgggtaga tccggagtca aaccatacgt cgagagtatc cggcactttc
    24901 acgtactgat cagcttcatc gccgaggatc tctttcgcat cgagatccca ccacgcctgg
    24961 atgccgtcaa cttccacgcg tttagccact tcttccatca gttcgagggt acgcggatgc
    25021 agctcttccg tgtctttatg cacgaacagt gacatcggta cgccccaggt gcgctgacgg
    25081 gagatacacc agtcaggacg gttagcaacc atcgactcga tacgcgcctg gccccagtcc
    25141 gggatccact gcacgccttt gatctctttc agtgactgcg cacgcagacc tttctgatcc
    25201 atgctgacga accactgcgg cgtcgcacgg aagatgatcg gcgttttgtg acgccagcag
    25261 cacggatagc tgtgctgcat tttctcaacg tgcaacagcg cgcctttttc ctgcagcagc
    25321 gcaacaacga tgtcgttcgc tttgaaaacg ttcacgccat ccagcgtcgg gtaagtaccc
    25381 ggcagataag tgccgtccgg gccaaccggg ttagcggttt ccaggccgta tttctgaccg
    25441 atcacatagt cgtccgggcc gtggccaggc gcggtgtgaa cggcaccggt accggcatcc
    25501 agggtaacgt gatcgccgag gattgccgga acgtcgaagc ccataaacgg atgggtaaag
    25561 cgcagcagct caagctctgc accttttacc gtgccgagaa tggtgtaatc ggtcacgccg
    25621 atacgctgca ttacgctttc aaccagatct tttgccagaa tcacggcctg accgtcgatc
    25681 tgcaccagca catagtcgaa atcaggtgca atagagattg cgcggttcgc aggcagagtc
    25741 cacggcgtag tggtccagat caccagcgag attgggccgt taacgttgct tacggcaaat
    25801 tttgctttca gtgcatcctg atcgaccgcc tggaaagcga cgtcgatgga cggagaagtt
    25861 ttgtcgtaat actcaacttc cgcttccgcc agcgcagaac ggcagtcaac gcaccagtgt
    25921 accggcttcg cgcctttgtg caggtgaccg ttgccgatga ttttgcccag cgcgcggatg
    25981 atattggctt cagttttgaa gtccatggtc aggtacgggt gcgaccagtc gcccagcacg
    26041 cccagacgga taaagtcttt gcgttgaccg tcaacctggg tcgcagcgta ttcgcggcac
    26101 ttggcgcgga actcggcagc ggtgaatttc tcacccggct taccgtattc ttgctctact
    26161 ttcagctcga tcggcagacc atgacagtcc cagccaggca catacggcga gtcataaccg
    26221 gaaagccctt tggacttcac gataatgtct ttcagaatct tgttaaccga gtgaccaata
    26281 tgaatgctgc cattcgcata aggagggcca tcatgcagaa tgaaggtttt tttgcctttt
    26341 ttagccgcac ggatgatgcc gtacagatca tcatcagtcc aacgcgccag cattcccggt
    26401 tcgcgcttgg cgagatcgcc acgcatcggg aaccctgttt ccggcaaatt cagggttgat
    26461 ttatagtcac tcatcagatt ctcggttccg tgtttcggtt tgattacata acaggcttaa
    26521 gccggttttg ttagcccaaa aaattcgcgg gcggttaatt catcacgcgc aatctgcgct
    26581 ttcagttcgt ccagcgacgc aaatcgctgc tcattgcgta tttttttacg cagcactact
    26641 tgtatatggc gaccgtaaag gtccattgca acatctaaca aatgcacttc cagctgctgg
    26701 cgaataccgg caaccgttgg gcgtgttccg atgtttgcca cgccgggtaa cggcttttca
    26761 ccgaggccca gcacttctac cgcataaacc cctttcaccg gggaaacctg acggcgtagc
    26821 ggtacattcg ccgtcgggaa acctatagtg cgccctaatt catcaccgtg gactacacgc
    26881 ccggagatgg caaacgggtg ccccagtaaa ctttctgcca gagccagatt gtcatccgcc
    26941 agcgcctgac gcacggcggt gctgctgata cgcacgccac cttcgcaaaa agtttgcgta
    27001 ctggtgatat cgaagccgta ttccgcgcca gctttctgta ataacaagaa atcgccttca
    27061 cgaccagcgc caaagcggaa atcatcacct acggcaagaa attttacccg caaatgcttc
    27121 accagaagat cgctgatgaa attttgcgcg gttaacgccg cgaaacgcct gtcgaaacgc
    27181 acgcacagca cgtaatcaac gccacactct gcaaggtaac gcagtttttc ccgcagccgg
    27241 gtcagccgtg ccggggcttt atcggtagca aacagttcca gtggttgagg ttcaaaaagc
    27301 atcaccatca ccggtaagtt gcgcttgcgc ccttcttcct gcaagccctg taacagcgcg
    27361 cgatgaccgc gatgcacgcc gtcgaaatta ccaatagtca gcacacaccc ttcttgcggg
    27421 gcctggctga gattatgtat gccgcgtatc agcttcatgt ctggctcaaa acagtgaaaa
    27481 tcgtccgagt ataccttgta cagcggtcaa ggttaaccgg cgattgagta ccgaaatcag
    27541 aaagaagtgg cttttcattg ccatggcgca aatcacggga agaaactgac cgcctgctgc
    27601 aatttttatc gcggaaaagc tgtattcaca ccccgcaagc tggtagaatc ctgcgccatc
    27661 actacgtaac gagtgccggc acattaacgg cgcttatttg cacaaatcca ttgacaaaag
    27721 aaggctaaaa gggcatattc ctcggccttt gaattgtcca tatagaacac atttgggagt
    27781 tggaccttgg ctaatatcaa atcagctaag aagcgcgcca ttcagtctga aaaggctcgt
    27841 aagcacaacg caagccgtcg ctctatgatg cgtactttca tcaagaaagt atacgcagct
    27901 atcgaagctg gcgacaaagc tgctgcacag aaagcattta acgaaatgca accaatcgtg
    27961 gaccgtcagg ctgctaaagg tctgatccac aaaaacaaag ctgcacgtca taaggctaac
    28021 ctgactgcac agatcaacaa actggcttaa ttgccaattt gctgaagctt tgtgaaaaag
    28081 cccgcgcaag cgggtttttt tatgcctgca cgcctggaaa tttcaaatga aaggcatatc
    28141 tcttttactt atttctgccg ccatatattt ccccaagtat aaaacattta acatttcatc
    28201 ataaaagtta aaatgtttta ttctctgata aaacgtgaac tgcataacaa aatacagaga
    28261 aacaaagcca aaaacgaatt aacgcaaact taaaacgaaa aattataatt gtcgccatca
    28321 ctctccttaa ctaacctcat tcccacaacg ttgtttaaat cattaataaa tatgcattaa
    28381 ggaaagtcag tatgtatttg cttagaaaat aaaagctttt tttctgagac aggcattctt
    28441 gttctggaga gaatataatg agttccgtca ttgaaactac tcaatcaact tcatccgatt
    28501 ccttaccttt aatacagcgg attagctacg gctcactgga tgttgccggt aatctgctct
    28561 actgttttgg ctctacctac attctttatt tctacaccga cgtagcaggg attagtctgg
    28621 cggttgcagg cataatccta ctgctggcgc gcattgtaga cggtattgat gcgccagtgt
    28681 ggggaatcat tatcgataaa acccattcgc gttatggaaa atgccgtcca tggtttttat
    28741 ggttaccatt accttttgct gtatttagtg cgctttcttt ctggtcaccc gatatcagca
    28801 tgacagggaa agctgtttat gcagcaatat cgtacatgtt agccagcatt ctgttcactg
    28861 ggttgaatac accattaagc gctatattac cgctgatgac gctgtcacca aaggaacgcc
    28921 tggtattaaa ctcatatcgc atgactggtg ggcaaattgg cgtactatta atgaatgcga
    28981 cagcattgcc attggtcgct ttccttggta atggtaacga taaagcaggc tttatatata
    29041 ctgccatagt ctttgccgta atatcctgtg ccttaacgtt gtttgccttt aaaaatattc
    29101 gcgaactgga taccgataaa atacagcaag aaccacgact gccgatgaaa aagagttttt
    29161 cggcaatgaa agggaactgg ccgtggctcc tgatggtagt ggcgaacctt atcttctgga
    29221 ttgccctaca gcagcgcaac actactattg tttactattt aacctacaat ctggatcgca
    29281 aagatctggt cccgctggtt aacagcctcg ccaccattca gatcctgttt atcattgcca
    29341 ttccgttctt cagtcgctat ctcaccaaaa cctggatttg gattaccggg ctgctggttg
    29401 cgatgttagg cgggggcctc atgtggctgg ctgccgacag catccctttg atgatcgccg
    29461 cctgggtact cgccaatatc ggcagcggta tcgcctgttc tatgcctttc gcgatgctcg
    29521 gtttcgccgt tgactttggt cgctggaaaa ccggcattaa agctaccggc attctgatcg
    29581 ccttcggcag caccttctgc atcaagatgg gcagtggtat cggtacagct ttcgctgcct
    29641 ttattatgga cagctttggt tatatcccca accaacaaca gacggctgcg gggttggaag
    29701 gtatcagcct ggcatttatc tgggtccctg cgctgctctt tgctctcgcg gctgtaccac
    29761 tgctcttctt tcgccagtat gaagcgatgg aaggtcgtat ccaacacgat ctgcaagccc
    29821 acaatcgttg aatttaatca acatgccact tgctcctggg aggaacaatt atgtcgttta
    29881 ttaagcaaga tccccaccgt ctggtctggc aacaagacga tcgttatctc tggattgaag
    29941 cctggggcga aaacagctta cgcgtgcgaa gcggccgcca tttaccggta atgcgcaatg
    30001 aaaactgggc gttaactcag gatcctggcg atgctgtcgc tcatatcacc tgggatgaaa
    30061 aacaggcaac gctgagaaat ggtaaaatca ctgccatcgt aaatcttcag ggccagctct
    30121 ctttctggcg taatgatgat aaatgcctgc tgcaagagtt ctggcgtcaa cgtggcgaaa
    30181 ttggtgaaga tgagtcagca cacggccaat atgtgagcgc tcttaatcta caggcccgtg
    30241 aattcaagcc tattcctggc gggaaattta cgattaaggc acgatttgaa gccaacgatg
    30301 gcgagaaact ctttggcatg ggtcagtatc agcaaccaaa cctggattta aaaggttgca
    30361 tgctggagct ggcacaacgt aactcgcagg cttctgttcc cttcctgcta tcaaatcagg
    30421 gttatggttt tctgtggaat aacccagcaa ttggccgcgt caccttcgcg caaaacggta
    30481 cggaatgggt agcagaagtc agtgaacaac tggactactg gatcactgcg ggcgatactc
    30541 cggctgagat cagtgaagcc tatgcacgag taaccggtac gccaccgatg atgcctgatt
    30601 acgcaatggg gttctggcaa tgcaagcttc gctatcgtaa tcaacaagaa ttgttagaag
    30661 tcgcacgcgg ctacaagcaa cgcaatcttc cgatctcggt gattgtcatc gatttcttcc
    30721 attggccgaa tcagggtgac tggatgttcg atctgcgtga ctggcccgat cccgatgcga
    30781 tgatcgccga attaaaagaa atgggtatcg aactgatggt ttcattctgg cctaccgtag
    30841 ataaccgtac cgagagttat cgggagatga aagaaaacgg ctggctagtc cacaccgagc
    30901 gtggtttgcc tatcaatatg gatttccttg gcaacaccac gttctttgat gcgacacatc
    30961 ctggcgcacg ggaatatgtc tggaacaaag ccaaacgcaa ttattacgat aaaggcgtga
    31021 aactgttctg gctggatgaa gccgagccag agttcggtgt ttacgattat gacaactatc
    31081 gttactacgc agggccagtg ctggaagtcg gtaatatcta tccgcgtatg tacgccaaaa
    31141 cgttcttcga tggcatgcag gcagccggag aaaagcaggt gatcaatttg ctacgctgcg
    31201 cctgggccgg tagtcagaaa tatggtgccc tggtgtggtc aggcgatatc cactcctcct
    31261 tccgttctct gcgcaatcag tttgctgcgg ggctgaacat gggaattgcc gggatcccgt
    31321 ggtggacgac ggatattggc ggtttccacg gtggcaacat acatgatcca aaatttcatg
    31381 aactgcttat tcgctggttc cagtgggggg tattcagccc agtaatgcgt ttacatggca
    31441 accgcgaccc gcaagttttg cctgaacaac cctatcgtga tggcatcgca caatgtccga
    31501 ctggcgcgcc gaatgaagtc tggagctatg gtgaagaaac cggtgaaatt ctcacttcct
    31561 gcctgcagtt gcgtgagaag ctgaaacctt atatcagtaa aattatggct gagacgcacg
    31621 agaagaacag cccggtaatg cgcacgatgt tctttgagtt tccagaccag gcagaaagct
    31681 ggaatattta tgatcagtac tgctttggcc cggaccttct cgttgcccct gtaatgcacg
    31741 aaggtacacg ttcacgtgat gtctggttgc ctgccggaga gacatggatc gatttttata
    31801 cccatgaaca ctacgcgggt gggcaaaagc ttcatcattg cgcgccgctg caccagatac
    31861 cggtattcat tcgtgagaaa gggaaatatc gccagttatt acccgtttaa aggacatcgt
    31921 ttgttcatta gcacgccccg tatatagcgg ggcgtgagtt ttaatatcta tcgtgaggcg
    31981 ggagaaaaca acgaagaata atccgtattg cagattcgct gtaccgccgg gtgctgaatc
    32041 atccgctcag caaaaatagc atggtactct tccatcacat tttcgacgcg accaatttct
    32101 acgacagttt tatcggcata aaagtcatat gcataaagcg ttggggcaac gaagattgca
    32161 ttgtgcatcg caccaaaagc tttcatcaac gcggcatcat caaactcgcc gaggatttct
    32221 acgtttaatc cctgggagtt aaaccagtta agcaatttgc gccctaacat tgaacgtcgc
    32281 ccaggaatca aaagccgccg ttcttccaga caagccggga acggtttttc cggtggtgga
    32341 tttgtacacc agaaactcac gccacattcg ccaattctca cggagaacag accttcctgc
    32401 tgcgtagaat ctatcggaca gtcggaaatg atcatatcca gtttatgctg acttaattgc
    32461 tccagcagca tttcgtgggt ggattcgaag cagcgaagat gaatcggctc gccttctacc
    32521 actgcggcgt taagtacgct actgaccagg cgtttagaaa gtgcatcagc cacgccaacg
    32581 tcaaacaata aattggattc tttgcgatag ttcacgatat ccagcatttc ctggcttaag
    32641 gtgaacattt tatcggcata gcgatagacc agctctccca gctcgctggg ctcgaggcca
    32701 cgtcccttgc gcttaaataa tttgccttgc aggcgctctt ccagcgcccg aatctgtccg
    32761 gtaatggttt gtggtgttaa ataaagcgcc tccgctgcgc caaccacgga accttcttta
    32821 tagacatgcc agaagtaata caagtggttg taattgatat gagacatgct catttctctc
    32881 cctgataaca atgaaaaggg agccgtttat ggctccccgg taaaccgtcc tgtcaaactg
    32941 atggacgcaa acgaacgcgt aaccagctgt atccaattac cgccgaagag atagaaccga
    33001 ccaggatacc gagtttcgcc cagttaatca gttctggatc tacgctacca aaggcaaggc
    33061 tggcaataaa gatagacata gtaaaaccga taccgcatag gatccccacc gccataattt
    33121 gctgataagt cgttccctca ggcagatgcg ccagtttcaa acgcagcgcc aaccagcaga
    33181 acagactaat acccagtggc ttgccaatca gcaaaccggc gatgatccct aatggcagaa
    33241 tggaggtcag gccatccagc gtcacgcctt gcagagatac ccccgcattg gcaaacgcaa
    33301 acaacggcaa aatcagatat gccacccacg gatgcaaaac atgctccaga cgtttagccg
    33361 gagagcgccc atgcttctct tttaacggaa taaagaagcc gacaatgacg ccagccagcg
    33421 ttgcatggac acctgatttc aacaccgctg tccacagtac cacgccaacc agaatataaa
    33481 cgcccgtgcg acgaacacca cagatattca gtaccgcgag tacggcaatc gctactgccg
    33541 caacgccgag agacgccatc gataagtcat tagtgtagaa caatgcgatg ataatgatgg
    33601 ccccaagatc gtcgataata gccagagcca tcaaaaagat cttcagcgct aacggaacac
    33661 gacttcccaa cagcgccagc acaccaagtg caaaggcaat gtcagtagcc gccgggatcg
    33721 cccacccttc gcgggtaatc ggatcggcat agttaaaggc cagatagagc aatgccggca
    33781 caatcatccc accaatagcg gcgataactg gaaatgcggc ctggcgtaag ctggctagcg
    33841 atccttgcat cagttcacgt ttaacttcca gaccgactaa caggaaaaat accgccatca
    33901 gcgcgtcatt tatccataac agcatgtttt tgttgatttc gagtgaacca acccggagct
    33961 gaaccggcgt ctccagaaag tcgtgatacc atccactggt tgcgccgcta ttggccataa
    34021 tcatcgccag gatagcggca atgataagaa taatgcctcc cgaggcatca ctgctaaaga
    34081 atcgatgcag atgtttcact ttttatttct ctttcaggtg aatagatcgt ttttactatt
    34141 ttaccccgca cgaatcatcg ttaaaatcag attatagtgt aggaataatt cgttttttac
    34201 gaaccattga aggatttgtg ccatcagcga tgagatgatt ttttgacggc tgtaagcgct
    34261 cacgagtgac cctaagccct gataatcata gcttaaggtg aggatttctg agggattaat
    34321 taggaaaagt aataataaaa tcgcactgtg ctatagctgt ttattccaga gatgcttgaa
    34381 aatatctaat gtggagacaa cattggcagg cgacattcgg tttaatgagg atagaaaggg
    34441 atgagactcc aggaagttga taaaacgagt cgcgcttgct aacgtcgaat accgcatttc
    34501 atttgctttc ttgtttcctg taaggttaca aacagccatt gaataatcac gggcggaaaa
    34561 tgtacgccaa acattatttc tcacgccacc actcaaaaac aaccgcggcg gcgtatcata
    34621 atgagtcgtt ttctcgccgc gttctttcac aaatgaaata tggcaccggc cttgatgaat
    34681 atgctgtaaa ttagccatca gacaaccacc taatataaag cactcgtgat caaggtagtg
    34741 ttgaatattt atagaatatg cttcagtcag tgctctaacc agttcaaaat ggcgatacgc
    34801 cggaagaacc ggtttcacca acatccccgg atcgttttga tgtaatatcg cccgaccata
    34861 atcaatatca aataaggtat cacgccgcat agtaagtaac tccttgcgtt gtacgtgatg
    34921 cgccagtgta cctggagaca aacgctcttc ataacttgat gcatattgat aatgcgactc
    34981 taccgctgat ggagaataat ttgttgaaat agcgacgact ctaccgcttt gttcttctgc
    35041 ggtaacaagg ctataaagat agttattact accgttaaac tttacgcgaa aagcgcgggt
    35101 agatagagaa atatcagaag cagaagagta ttggtaactt tctttatagt ttacttcccg
    35161 ggctagtttc atcaactcac ggttaagccc tgtgctatta agaagtaata atttacggat
    35221 atctgcaagt gaagcgcctt gatcaatcat caatgctaaa tgtgttcttc gcggcgttgt
    35281 atttacatga tccagagtga taaaagttct ggaacaatga tgacaataca ttctcctctg
    35341 cccctgagct gaatagccat actttttcag agatgtgctt ccgcaagctg aacattgtgg
    35401 aaccaaccct ctccatgaat gattaacacg attacgataa atattaagag atcgttcaga
    35461 tatcacagga aacaagtaac cgcattcccg acataaaata ttttttcctt gtgcaatata
    35521 atcctggcta ttcaacaacc ccaggttttt gcatcctggt gttttgcaac aatcaacatt
    35581 tacgttggta aacatattca ctccctgcga atacccggat attatcgtca tatgtcaaag
    35641 aaaagaacct gactcaatgt gtaacattga atcaggcggg catggccctg aaaggcatac
    35701 attacattta ttatggttat cagattagcc ccaggttacc gcttccggtt tatcacctct
    35761 ggctttcatt aatgaatcat tatcaatatc ggctttatac tttaagaact taccatacat
    35821 ttgtttttca atgtctggat ttttacctat ctgattaatg gtctcaaaac gatcggtttt
    35881 gaggttgtat aggtactttg gcttattgtt acgatcgatg atcattttcc agtcaccatc
    35941 acgtatcgcc cattcgtcgg ttggatcatc ctggaatggc atgtcaattc cgaagattaa
    36001 tggcttttca cgttttagcg ctt
//
LOCUS       DAMZFT010000037        35756 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.037, whole genome shotgun sequence.
ACCESSION   DAMZFT010000037 DAMZFT010000000
VERSION     DAMZFT010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 35756)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35756)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35756
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.037"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(<1..965)
                     /gene="coaBC"
                     /locus_tag="PN977_001924"
     CDS             complement(<1..965)
                     /gene="coaBC"
                     /locus_tag="PN977_001924"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418096.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HDI6041955.1"
                     /translation="MSLAGKKIVLGVSGGIAAYKTPELVRRLRDRGADVRVAMTEAAK
                     AFITPLSLQAVSGYPVSDSLLDPAAEAAMGHIELGKWADLVILAPATADLIARVAAGM
                     ANDLVSTICLATPAPVAVLPAMNQQMYRAAATQHNLEVLASRGLLIWGPDSGSQACGD
                     IGPGRMLDPLTIVDMAVAHFSPVNDLKHLNIMITAGPTREPLDPVRYISNHSSGKMGF
                     AIAAAAARRGANVTLVSGPVSLPTPPFVNRVDVMTALEMEAAVNASVQQQNIFIGCAA
                     VADYRAATVAPEKIKKQATQGDELTIKMVKNPDIVAGVAALKDHRP"
     gene            1137..1805
                     /gene="radC"
                     /locus_tag="PN977_001925"
     CDS             1137..1805
                     /gene="radC"
                     /locus_tag="PN977_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HDI6041956.1"
                     /translation="MKNNAQLLMPREKMLKFGISALTDVELLALFLRTGTRGKDVLTL
                     AKEMLENFGSLYGLLTSEYEQFSGVHGIGVAKFAQLKGIAELARRYYNVRMREESPLL
                     SPEMTREFLQSQLTGEEWEIFMVIFLDSQHRVITHSRLFSGTLNHVEVHPREIIREAI
                     KINASALILAHNHPSGCAEPSKADKLITERIIKSCQFMDLRVLDHIVIGRGEYVSFAE
                     RGWI"
     gene            2022..2258
                     /gene="rpmB"
                     /locus_tag="PN977_001926"
     CDS             2022..2258
                     /gene="rpmB"
                     /locus_tag="PN977_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008457442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HDI6041957.1"
                     /translation="MSRVCQVTGKRPVTGNNRSHALNATKRRFLPNLHSHRFWVESEK
                     RFVTLRVSAKGMRVIDKKGIDTVLAELRARGEKY"
     gene            2279..2446
                     /gene="rpmG"
                     /locus_tag="PN977_001927"
     CDS             2279..2446
                     /gene="rpmG"
                     /locus_tag="PN977_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HDI6041958.1"
                     /translation="MAKGIREKIKLVSSAGTGHFYTTTKNKRTKPEKLELKKFDPVVR
                     QHVIYKEAKIK"
     gene            2544..3353
                     /gene="mutM"
                     /locus_tag="PN977_001928"
     CDS             2544..3353
                     /gene="mutM"
                     /locus_tag="PN977_001928"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HDI6041959.1"
                     /translation="MPELPEVETSRRGIEPHLVGATILHAVVRNGRLRWPVSEEIYRL
                     SDQPVLSVQRRAKYLLLELPEGWIIIHLGMSGSLRILPEELPPEKHDHVDLVMSNGKV
                     LRYTDPRRFGAWLWTKELEGHNVLAHLGPEPLSDDFNGEYLHQKCAKKKTAIKPWLMD
                     NKLVVGVGNIYASESLFAAGIHPDRLASSLSLAECELLARVIKAVLLRSIEQGGTTLK
                     DFLQSDGKPGYFAQELQVYGRKGEPCRVCGTPIVATKHAQRATFYCRQCQK"
     gene            complement(3392..3871)
                     /gene="coaD"
                     /locus_tag="PN977_001929"
     CDS             complement(3392..3871)
                     /gene="coaD"
                     /locus_tag="PN977_001929"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HDI6041960.1"
                     /translation="MQKRAIYPGTFDPITNGHIDIVTRATQMFDHVILAIAASPSKKP
                     MFTLEERVALAQQATAHLGNVEVVGFSDLMANFARNQHATVLIRGLRAVADFEYEMQL
                     AHMNRHLMPELESVFLMPSKEWSFISSSLVKEVARHQGDVTHFLPENVHQALMAKLA"
     gene            complement(3879..5156)
                     /gene="waaA"
                     /locus_tag="PN977_001930"
     CDS             complement(3879..5156)
                     /gene="waaA"
                     /locus_tag="PN977_001930"
                     /EC_number="2.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) 3-deoxy-D-manno-octulosonic acid
                     transferase"
                     /protein_id="HDI6041961.1"
                     /translation="MLELLYTALLYLIQPLIWIRLWVRGRKAPAYRKRWGERYGFYRH
                     PLKPGGIMLHSVSVGETLAAIPLVRALRHRYPDLPITVTTMTPTGSERVQSAFGKDVQ
                     HVYLPYDLPDALNRFLNKVDPKLVLIMETELWPNLIAALHKRKIPLVIANARLSARSA
                     AGYAKLGKFVRRLLRRITLIAAQNEEDGARFVSLGAKNNQVTVTGSLKFDISVTPQLA
                     AKAVTLRRQWAPHRPVWIATSTHEGEESVVIAAHQALLQQFPNLLLILVPRHPERFPD
                     AINLVRQAGLSYITRSSGEVPSTSTQVVVGDTMGELMLLYGIADLAFVGGSLVERGGH
                     NPLEAAAHAIPVLMGPHTFNFKDICARLEQASGLITVTDATTLAKEVSSLLTDADYRS
                     FYGRHAVEVLYQNQGALQRLLQLLEPYLPPKTH"
     gene            5569..6627
                     /gene="rfaQ"
                     /locus_tag="PN977_001931"
     CDS             5569..6627
                     /gene="rfaQ"
                     /locus_tag="PN977_001931"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptosyltransferase
                     RfaQ"
                     /protein_id="HDI6041962.1"
                     /translation="MDKPFRRILLIKMRFHGDMLLTTPVISSLKKNYPDAKIDVLLYQ
                     DTIPILSENPEINALYGIKNKKAKASEKIANFFHLIKILRANKYDLIVNLTDQWMVAI
                     LVRLLNARVKISQDYHHRQSAFWRNSFTHLVPLQGGNVVESNLSVLTPLGLESLVKQT
                     TMSYPPASWKRMRRELDHAGVGQNYVVIQPTARQIFKCWDNAKFSAVIDALHARGYEV
                     VLTSGPDKDDLACVNEIAQGCQTPPVTALAGKVTFPELGALIDHAQLFIGVDSAPAHI
                     AAAVNTPLISLFGATDHIFWRPWSNNMIQFWAGDYREMPTRDQRDRNEMYLSVIPAAD
                     VIAAVDKLLPSSTTGTSL"
     gene            6624..7748
                     /locus_tag="PN977_001932"
     CDS             6624..7748
                     /locus_tag="PN977_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HDI6041963.1"
                     /translation="MIVAFCLYKYFPFGGLQRDFMRIAQTVAARGHHVRVYTQSWEGE
                     CPDVFELIKVPVNSHTNHGRNAEYFAWVQKHLREHPVDRVVGFNKMPGLDVYYAADVC
                     YAEKVAQEKGFFYRLTSRYRHYAAFERATFEQGKPTQLLMLTDKQIADFQKHYQTEAE
                     RFHILPPGIYPDRKYSQQPANSREIFRKKNGITEQQYLLLQVGSDFTRKGVDRSIEAL
                     ASLPDSLRHNTLLYVVGQDKPRKFEALAEKRGVRSNVHFFSGRNDVSELMAAADLLLH
                     PAYQEAAGIVLLEAITAGLPVLTTAVCGYAHYIVDANCGEAIAEPFRQETLNEILRKA
                     LTQSSLRQAWAENARHYADTQDLYSLPEKAADIITGGLDG"
     gene            7741..8538
                     /gene="rfaP"
                     /locus_tag="PN977_001933"
     CDS             7741..8538
                     /gene="rfaP"
                     /locus_tag="PN977_001933"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(I) kinase RfaP"
                     /protein_id="HDI6041964.1"
                     /translation="MVELKEPFATLWRGKDPFEEVKTLQGEVFRELETRRTLRFEMAG
                     KSYFLKWHRGTTLKEIIKNLLSLRMPVLGADREWNAIHRLRDVGVDTMYGVAFGEKGI
                     NPLTRTSFIITEDLTPTISLEDYCADWATNPPDVRVKRMLIKRVATMVRDMHAAGINH
                     RDCYICHFLLHLPFSGKEEELKISVIDLHRAQLRTRVPCRWRDKDLIGLYFSSMNIGL
                     TQRDIWRFMKVYFVAPLKDILKQEQGLLSQAEEKATKIRERTIRKSL"
     gene            8554..9570
                     /gene="waaO"
                     /locus_tag="PN977_001934"
                     /gene_synonym="rfaI"
     CDS             8554..9570
                     /gene="waaO"
                     /locus_tag="PN977_001934"
                     /gene_synonym="rfaI"
                     /EC_number="2.4.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     3-alpha-galactosyltransferase"
                     /protein_id="HDI6041965.1"
                     /translation="MSAHYFNPQEMINKTIIFDERPEASVASSFHVAYGIDQNFLFGC
                     GVSITSVLLHNNDVSFVFHVFIDDIPDADIQRLSQLAKSYHTCIQIHLVNCERLKVLP
                     TTKNWSIAMYFRFVIADYFIDQQDKILYLDADIACQGTLKPLITMDLANNVAAVVTER
                     DANWWSLRAQSLQCNELEKGYFNSGVLLINTPAWAQESVSAKAMSMLADKAVVSRLTY
                     MDQDILNLILLGKVKFIDAKYNTQFSLNYELKKSFICPINDEAVLIHYVGPTKPWHYW
                     AGYPSAQPFIKAKEASPWKNEQLMRPINSNYARYCAKHNFKQNKPINGIMNYIYYFYL
                     KIIK"
     gene            9587..10582
                     /locus_tag="PN977_001935"
     CDS             9587..10582
                     /locus_tag="PN977_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001001722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide 1,2-glucosyltransferase"
                     /protein_id="HDI6041966.1"
                     /translation="MNEFIKERFSYLADNKKENVPELNVSYGIDKNFLYGAGVSISSV
                     LINNSDINFVFHVFTDYVDDDYLKSFNETAKQFNTSIIVYLIDPKYFADLPTSQFWSY
                     ATYFRVLSFEYLSESISTLLYLDADVVCKGSLKPLTKIIFKDEFAAVIPDNDSTQAAC
                     AKRLNIPEMNGRYFNAGVIYVNLKKWHEANLTPYLLTLLRGETKYGSLKYLDQDALNI
                     AFNMNNIYLAKDFDTIYTLKNELHDRSHRKYQQTITDKTVLIHYTGITKPWHSWAGYP
                     SASYFNIAREQSPWKKYPLKEARTVAEMQKQYKHLFAHGEYIKGITSLIKYKLKK"
     gene            10592..11284
                     /gene="rfaY"
                     /locus_tag="PN977_001936"
     CDS             10592..11284
                     /gene="rfaY"
                     /locus_tag="PN977_001936"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000633080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(II) kinase RfaY"
                     /protein_id="HDI6041967.1"
                     /translation="MITSIRYRGFSFYYKNNDNKYKEIFDEILAYNFKTVKVLRNIDD
                     TKVSLIDTKYGRYVFKVFAPKTKRNERFLKSFVKGDYYQNLIVETDRVRSAGLTFPND
                     FYFLAERKIFNYASVFIMLIEYVEGVELNDMPIIPEDVKAEIKASMEKLHALNMLSGD
                     PHRGNFIVSKDGVRIIDLSGKSCTAERKARDRLAMERHLGIANEIKDYGYYSVIYRTK
                     LRKFIKKLKGKA"
     gene            11310..12338
                     /locus_tag="PN977_001937"
     CDS             11310..12338
                     /locus_tag="PN977_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001592907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactose--(galactosyl) LPS
                     alpha1,2-galactosyltransferase"
                     /protein_id="HDI6041968.1"
                     /translation="MDLLAESITEVAVSGEIANTDRVLNIAYGIDRNFLFGAAVSMQS
                     VVMHNPDLAVKFHLFTDYIDEDYLQRVNAFTSKNANVEVRIYKVSSAFIDIFPSLKQW
                     SYATFFRLVAFQYLSETIENLLYIDADVICKGSLAGLLDINFDGDKFAAVIKDVPFMQ
                     EKPAKRLAIEGLPGNYFNAGVVYLQLEAWAKNDFMNKAIAMLASDPQHTKYKCLDQDI
                     LNILFFGHCIFISGDYDCFYGIDYELKNKSDEDYKKTITDDTKLIHYVGVTKPWNDWT
                     NYPCQKYFNEAYQASCWNDVAFIPATNEKQYQVKYQHAKKNGDTFNAFIYFIKFKLNK
                     YKRKLFRQ"
     gene            12420..13403
                     /locus_tag="PN977_001938"
     CDS             12420..13403
                     /locus_tag="PN977_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016246416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HDI6041969.1"
                     /translation="MSNDYPLVSIIIPTYNSSDYITETLTKLEKQTYPNFEIVIVNDG
                     SKDNTSNVLREYGLTHSRLIILNKENGGVSSARNTGIRKAQGQFICFMDDDDEIDPNY
                     LLKMYSRQHETGGDAIYCGLYGHHIKNGVTYSPINTDFNEGSLLFDFFYKKVRFHIGC
                     LFIRKQLLEDNNLFFDEDLRLGEDLDFIYRLLITCDMYAVPYYMYKHNYRENSLMNSC
                     RTITHYRHESFAHERIYSSVMQLYKGNRKEEIHTLLSKNRTYHKTRYLWNVLLNGDFE
                     LLNQLVESNEKELKDCNLLGKRDKRRAKILASKNYILWRMVRLVNRKKNKR"
     gene            13449..14702
                     /locus_tag="PN977_001939"
     CDS             13449..14702
                     /locus_tag="PN977_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012311869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HDI6041970.1"
                     /translation="MSFCWKEINSGVKSLILILCMLSLMTLSLWDDVATKFLHAAGII
                     STLYFLATAKKTITNNPTLLIFISLCLLGIVNIIWYSHYKVSGSVYTNAYRGPMETGK
                     IALCSAFIFLVLFAKDEMRTKIKFGKLILFASLATQLLFFAHAMWQHFYLNVDRVALS
                     ASHATTAGYIILFPSLLASILILKCDFRHKTTLYTINFMLSLCAVIVTETRAAILVFP
                     FFALILIVMDSYINKRINYKLYCFIAIALLAGVFSFKDTLLMRMNDLNNDLVNYSHDN
                     TRTSVGARLAMYEVGLKTYSPIGQSLEKRAEKIHELEEKEPRLSGALPFVDSHLHNDL
                     IDTLSTRGIPGVVLTILAFSAIFIYALRTAKEPYILILLFSLLVVGLSDVILFSKPVP
                     TAVFVTIILLCAYFKAQSDQCLLDK"
     gene            complement(14772..15743)
                     /gene="rfaC"
                     /locus_tag="PN977_001940"
     CDS             complement(14772..15743)
                     /gene="rfaC"
                     /locus_tag="PN977_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase RfaC"
                     /protein_id="HDI6041971.1"
                     /translation="MRVLIVKTSSMGDVLHTLPALTDAQQAIPGIKFDWVVEEGFTQI
                     PSWHAAVERVIPVAIRRWRKAWFSAPIKAERKAFRETLQAENYDAVIDAQGLVKSAAL
                     VTRLAHGVKHGMDWQTAREPLASLFYNRKHHIAKQQHAVERTRELFAKSLGYSKPQTQ
                     GDYAIAQHFLTNLPTDAGEYAVFLHATTRDDKHWPEEHWRELIGLLADSGIRIKLPWG
                     APHEEERAKRLAEGFAYVEVLPKMSLEGVARVLAGAKFVVSVDTGLSHLTAALDRPNI
                     TVYGPTDPGLIGGYGKNQMVCRAPGNELSQLTANAVKRFIEENAAMI"
     gene            complement(15747..16793)
                     /gene="rfaF"
                     /locus_tag="PN977_001941"
     CDS             complement(15747..16793)
                     /gene="rfaF"
                     /locus_tag="PN977_001941"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-heptose--LPS heptosyltransferase RfaF"
                     /protein_id="HDI6041972.1"
                     /translation="MKILVIGPSWVGDMMMSQSLYRTLQARYPQAIIDVMAPAWCRPL
                     LSRMPEVNEAIPMPLGHGALEIGERRKLGHSLREKRYDRAYVLPNSFKSALVPFFAGI
                     PHRTGWRGEMRYGLLNDVRVLDKEAWPLMVERYVALAYDKGIMRTAQDLPQPLLWPQL
                     QVSEGEKSYTCNQFSLSSERPMIGFCPGAEFGPAKRWPHYHYAELAKQLIDEGYQVVL
                     FGSAKDHEAGNEILAALNTEQQAWCRNLAGETQLDQAVILIAACKAIVTNDSGLMHVA
                     AALNRPLVALYGPSSPDFTPPLSHKARVIRLITGYHKVRKGDAAEGYHQSLIDITPQR
                     VLEELNALLLQEEA"
     gene            complement(16803..17735)
                     /gene="rfaD"
                     /locus_tag="PN977_001942"
     CDS             complement(16803..17735)
                     /gene="rfaD"
                     /locus_tag="PN977_001942"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HDI6041973.1"
                     /translation="MIIVTGGAGFIGSNIVKALNDKGITDILVVDNLKDGTKFVNLVD
                     LDIADYMDKEDFLIQIMAGEEFGDVEAIFHEGACSSTTEWDGKYMMDNNYQYSKELLH
                     YCLEREIPLLYASSAATYGGRTSDFIESREYEKPLNVYGYSKFLFDEYVRQILPEANS
                     QIVGFRYFNVYGPREGHKGSMASVAFHLNTQLNNGESPKLFEGSENFKRDFVYVGDVA
                     DVNLWFLENGVSGIFNLGTGRAESFQAVADATLAYHKKGQIEYIPFPDKLKGRYQAFT
                     QADLTNLRAAGYDKPFKTVAEGVTEYMAWLNRDA"
     gene            17949..19145
                     /gene="kbl"
                     /locus_tag="PN977_001943"
     CDS             17949..19145
                     /gene="kbl"
                     /locus_tag="PN977_001943"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HDI6041974.1"
                     /translation="MRGEFYQQLTNDLETARAEGLFKEERIITSAQQADITVADGSHV
                     INFCANNYLGLANHPDLIAAAKAGMDSHGFGMASVRFICGTQDSHKELEQKLAAFLGM
                     EDAILYSSCFDANGGLFETLLGAEDAIISDALNHASIIDGVRLCKAKRYRYANNDMQE
                     LEARLKEAREAGARHVLIATDGVFSMDGVIANLKGVCDLADKYDALVMVDDSHAVGFV
                     GENGRGSHEYCEVMGRVDIITGTLGKALGGASGGYTAARKEVVEWLRQRSRPYLFSNS
                     LAPAIVAASIKVLEMVEAGSELRDRLWANARQFREQMSAAGFTLAGADHAIIPVMLGD
                     AVVAQKFARELQKEGIYVTGFFYPVVPKGQARIRTQMSAAHTPEQITRAVEAFTRIGK
                     QLGVIA"
     gene            19155..20180
                     /gene="tdh"
                     /locus_tag="PN977_001944"
     CDS             19155..20180
                     /gene="tdh"
                     /locus_tag="PN977_001944"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HDI6041975.1"
                     /translation="MKALSKLKAEEGIWMTDVPVPELGHNDLLIKIRKTAICGTDVHI
                     YNWDEWSQKTIPVPMVVGHEYVGEVVGIGQEVKGFKIGDRVSGEGHITCGHCRNCRGG
                     RTHLCRNTIGVGVNRPGCFAEYLVIPAFNAFKIPDNISDDLASIFDPFGNAVHTALSF
                     DLVGEDVLVSGAGPIGIMAAAVAKHVGARNVVITDVNEYRLELARKMGITRAVNVAKE
                     NLNDVMTELGMTEGFDVGLEMSGAPPAFRTMLDTMNHGGRIAMLGIPPSDMSIDWTKV
                     IFKGLFIKGIYGREMFETWYKMAALIQSGLDLSPIITHRFSIDDFQKGFDAMRSGQSG
                     KVILSWD"
     gene            20419..21453
                     /gene="waaH"
                     /locus_tag="PN977_001945"
     CDS             20419..21453
                     /gene="waaH"
                     /locus_tag="PN977_001945"
                     /EC_number="2.4.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucuronate:LPS(HepIII) glycosyltransferase"
                     /protein_id="HDI6041976.1"
                     /translation="MMNSTNKLSVIIPLYNAGDDFSACMESLIAQTWTALEIIIINDG
                     STDNSVEIAKHYAENYPHVRLLHQANAGASVARNRGIEVATGKYVAFVDADDEVYPTM
                     YETLMTMALEDDLDVAQCNADWCFRETGETWQSIPTDRLRSTGVLTGPDWLRMGLSSR
                     RWTHVVWMGVYRRDVIVKNNIKFIAGLHHQDIVWTTEFMFNALRARYTEQSLYKYYLH
                     NTSVSRLNRQGNKNLNYQRHYIKITRLLEKLNRNYADKITIYPEFHQQITYEALRVCH
                     AVRKEPDIITRQRMIAEIFTSGMYKRLITNVRSVKVGYQALLWSFRLWQWRDKTRSHH
                     RITRSAFNLR"
     gene            complement(21440..22399)
                     /locus_tag="PN977_001946"
     CDS             complement(21440..22399)
                     /locus_tag="PN977_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent polysaccharide deacetylase family
                     protein"
                     /protein_id="HDI6041977.1"
                     /translation="MFPFRRNVLAFAALLALSSPVIAGKLAIVIDDFGYRPHNENQVL
                     AMPSAISVAVLPDSPHAREMATKAHNSGHEVLIHLPMAPLSKQPLEKNTLRPEMSSDE
                     IERIIRSAVNNVPYAVGINNHMGSKMTSNLFGMQKVMQALERYNLYFLDSVTIGNTQA
                     MRAAQGTGVKVIKRKVFLDDSQNEADIRVQFNRAIDLARRNGSTIAIGHPHPSTVRVL
                     QQMVYNLPPDITLVKASSLLNEPQVDTSTPPKNTVPDTPRNPFRGVKLCKPKKPLEPV
                     YANRFFEVLSESISQSTLIVYFQHQWQGWGKQPQAAKLNASAN"
     gene            complement(22403..23686)
                     /gene="envC"
                     /locus_tag="PN977_001947"
     CDS             complement(22403..23686)
                     /gene="envC"
                     /locus_tag="PN977_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator EnvC"
                     /protein_id="HDI6041978.1"
                     /translation="MRGKAINTMTRAVKPRRFAIRPIIYASVLSAGVLLCAFSAHADE
                     RDQLKSIQADIAAKERAVRQKQQQRASLLAQLKKQEEAISEATRKLRETQNTLNQLNK
                     QIDEMNASIAKLEQQKAAQERSLAAQLDAAFRQGEHTGIQLILSGEESQRGQRLQAYF
                     GYLNQARQETIAQLKQTREEVAMQRAELEEKQSEQQTLLYEQRAQQAKLTQALSERKK
                     TLAGLESSIQQGQQQLSELRANESRLRNSIARAEAAAKARAEREAREAQAVRDRQKEA
                     TRKGTTYKPTESEKSLMSRTGGLGAPRGQAFWPVRGPTLHRYGEQLQGELRWKGMVIG
                     ASEGTEVKAIADGRVILADWLQGYGLVVVVEHGKGDMSLYGYNQSALVSVGSQVRAGQ
                     PIALVGSSGGQGRPSLYFEIRRQGQAVNPQPWLGR"
     gene            complement(23696..25240)
                     /gene="gpmM"
                     /locus_tag="PN977_001948"
                     /gene_synonym="pgmI"
     CDS             complement(23696..25240)
                     /gene="gpmM"
                     /locus_tag="PN977_001948"
                     /gene_synonym="pgmI"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HDI6041979.1"
                     /translation="MSVSKKPMVLVILDGYGYREEQQDNAIFSAKTPVMDALWANRPH
                     TLIDASGLEVGLPDRQMGNSEVGHVNLGAGRIVYQDLTRLDVEIKDRAFFANPVLTGA
                     VDKAKNAGKAVHIMGLLSAGGVHSHEDHIMAMVELAAERGAEKIYLHAFLDGRDTPPR
                     SAESSLKKFEEKFAALGKGRVASIIGRYYAMDRDNRWDRVEKAYDLLTLAQGEFQADT
                     AVAGLQAAYARDENDEFVKATVIRAEGQPDAAMEDGDALIFMNFRADRAREITRAFVN
                     ADFDGFARKKVVNVDFVMLTEYAADIKTAVAYPPASLVNTFGEWMAKNDKTQLRISET
                     EKYAHVTFFFNGGVEESFKGEDRILINSPKVATYDLQPEMSSAELTEKLVAAIKSGKY
                     DTIICNYPNGDMVGHTGVMEAAVKAVEALDHCVEEVAKAVESVGGQLLITADHGNAEQ
                     MRDPATGQAHTAHTNLPVPLIYVGDKNVKAVEGGKLSDIAPTMLSLMGMEIPQEMTGK
                     PLFIVE"
     gene            25485..25916
                     /locus_tag="PN977_001949"
     CDS             25485..25916
                     /locus_tag="PN977_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HDI6041980.1"
                     /translation="MQEIMQFVGRHPILSIAWIALLVAVLVTTFKSLTSKVKVITRGE
                     ATRLINKEDAVVVDLRQRDDFRKGHIAGSINLLPSEIKANNVGELEKHKDKPVIVVDG
                     SGMQCQEPANALTKAGFAQVFVLKEGVAGWAGENLPLVRGK"
     gene            26058..26309
                     /gene="grxC"
                     /locus_tag="PN977_001950"
     CDS             26058..26309
                     /gene="grxC"
                     /locus_tag="PN977_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HDI6041981.1"
                     /translation="MANVEIYTKETCPYCHRAKALLSSKGVSFQELPIDGNAAKREEM
                     IKRSGRTTVPQIFIDAQHIGGCDDLYALDARGGLDPLLK"
     gene            26372..26839
                     /gene="secB"
                     /locus_tag="PN977_001951"
     CDS             26372..26839
                     /gene="secB"
                     /locus_tag="PN977_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015369180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HDI6041982.1"
                     /translation="MSEQNNTEMTFQIQRIYTKDISFEAPNAPHVFQKDWQPEVKLDL
                     DTASSQLADDVYEVVLRVTVTASLGEETAFLCEVQQGGIFSIAGIEGTQMAHCLGAYC
                     PNILFPYARECITSMVSRGTFPQLNLAPVNFDALFMNYLQQQAGEGTEEHQDA"
     gene            26839..27858
                     /gene="gpsA"
                     /locus_tag="PN977_001952"
     CDS             26839..27858
                     /gene="gpsA"
                     /locus_tag="PN977_001952"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HDI6041983.1"
                     /translation="MNQRNASMTVIGAGSYGTALAITLARNGHEVVLWGHDPEHIATL
                     ERDRCNAAFLPDVPFPDTLHLESDLATALAASRNILVVVPSHVFGEVLRQIKPLMRPD
                     ARLVWATKGLEAETGRLLQDVAREALGDQIPLAVISGPTFAKELAAGLPTAISLASTD
                     QTFADDLQQLLHCGKSFRVYSNPDFIGVQLGGAVKNVIAIGAGMSDGIGFGANARTAL
                     ITRGLAEMSRLGAALGADPATFMGMAGLGDLVLTCTDNQSRNRRFGMMLGQGMDVQSA
                     QEKIGQVVEGYRNTKEVRELAHRFGVEMPITEEIYQVLYCGKNAREAALTLLGRARKD
                     ERSSH"
     gene            27938..28759
                     /gene="cysE"
                     /locus_tag="PN977_001953"
     CDS             27938..28759
                     /gene="cysE"
                     /locus_tag="PN977_001953"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HDI6041984.1"
                     /translation="MSCEELEIVWNNIKAEARTLADCEPMLASFYHATLLKHENLGSA
                     LSYMLANKLSSPIMPAIAIREVVEEAYAADPEMIASAACDIQAVRTRDPAVDKYSTPL
                     LYLKGFHALQAYRIGHWLWNQGRRALAIFLQNQVSVTFQVDIHPAAKIGRGIMLDHAT
                     GIVVGETAVIENDVSILQSVTLGGTGKSGGDRHPKIREGVMIGAGAKILGNIEVGRGA
                     KIGAGSVVLQPVPPHTTAAGVPARIVGKPDSDKPSMDMDQHFNGINHTFEYGDGI"
     gene            complement(28812..29285)
                     /gene="trmL"
                     /locus_tag="PN977_001954"
     CDS             complement(28812..29285)
                     /gene="trmL"
                     /locus_tag="PN977_001954"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HDI6041985.1"
                     /translation="MLNIVLYEPEIPPNTGNIIRLCANTGFRLHIIEPMGFAWDDKRL
                     RRAGLDYHEFTAVTRHHDYRAFLEAENPQRLFALTTKGTPAHSAVSYQDGDYLMFGPE
                     TRGLPASILDALPAEQKIRIPMVPDSRSMNLSNAVSVVVYEAWRQLGYPGAVLRD"
     gene            complement(29333..30523)
                     /gene="lldD"
                     /locus_tag="PN977_001955"
     CDS             complement(29333..30523)
                     /gene="lldD"
                     /locus_tag="PN977_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent L-lactate dehydrogenase"
                     /protein_id="HDI6041986.1"
                     /translation="MIISAASDYRAAAQRILPPFLFHYMDGGAYSEYTLRRNVEDLSE
                     VALRQRILKNMSDLSLETTLFNEKLSMPVALAPVGLCGMYARRGEVQAAKAADAHGIP
                     FTLSTVSVCPIEEVAPAIKRPMWFQLYVLRDRGFMRNALERAKAAGCSTLVFTVDMPT
                     PGARYRDAHSGMSGPNAAMRRYLQAVTHPQWAWDVGLNGRPHDLGNISAYLGKPTGLE
                     DYIGWLGNNFDPSISWKDLEWIRDFWDGPMVIKGILDPEDARDAVRFGADGIVVSNHG
                     GRQLDGVLSSARALPAIADAVKGDIAILADSGIRNGLDVVRMIALGADTVLLGRAFLY
                     ALATAGQAGVANLLNLIEKEMKVAMTLTGAKSISEITQDSLVQELSKAPAAALAPMAK
                     GNAA"
     gene            complement(30520..31296)
                     /gene="lldR"
                     /locus_tag="PN977_001956"
     CDS             complement(30520..31296)
                     /gene="lldR"
                     /locus_tag="PN977_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LldR"
                     /protein_id="HDI6041987.1"
                     /translation="MIVLPRRLSDEVADRVRALIDEKNLEAGMKLPAERQLAMQLGVS
                     RNSLREALAKLVSEGVLLSRRGGGTFIRWRHDTWSEQNIVQPLKTLMADDPDYSFDIL
                     EARYAIEASTAWHAAMRATPGDKEKIQLCFEATLSEDPDLASQADVRFHLAIAEASHN
                     IVLLQTMRGFFDVLQSSVKHSRQRMYLVPPVFSQLTEQHQAVIDAIFAGDADGARKAM
                     MAHLSFVHTTMKRFDEDQARHARITRLPGDHNEHSREKNA"
     gene            complement(31296..32951)
                     /gene="lldP"
                     /locus_tag="PN977_001957"
     CDS             complement(31296..32951)
                     /gene="lldP"
                     /locus_tag="PN977_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HDI6041988.1"
                     /translation="MTLWQQNYDPAGNIWLSSLIASLPILFFFFALIKLKLKGYVAAS
                     WTVAIALAVALLFYKMPVANALASVVYGFFYGLWPIAWIIIAAVFVYKISVKTGQFDI
                     IRSSILSITPDQRLQMLIVGFCFGAFLEGAAGFGAPVAITAALLVGLGFKPLYAAGLC
                     LIVNTAPVAFGAMGIPILVAGQVTGIDSFEIGQMVGRQLPFMTIIVLFWIMAIMDGWR
                     GIKETWPAVVVAGGSFAIAQYLSSNFIGPELPDIISSLVSLLCLTLFLKRWQPVRVFR
                     FGDLGASQVDMTLAHTGYTAGQVLRAWTPFLFLTATVTLWSIPPFKALFASGGALYEW
                     VINIPVPYLDKLVARMPPVVSEATAYAAVFKFDWFSATGTAILFAALLSIVWLKMKPS
                     DAISTFGSTLKELALPIYSIGMVLAFAFISNYSGLSSTLALALAHTGHAFTFFSPFLG
                     WLGVFLTGSDTSSNALFAALQATAAQQIGVSDLLLVAANTTGGVTGKMISPQSIAIAC
                     AAVGLVGKESDLFRFTVKHSLIFTCMVGVITTLQAYVLTWMIP"
     gene            complement(33249..>35756)
                     /locus_tag="PN977_001958"
     CDS             complement(33249..>35756)
                     /locus_tag="PN977_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YadA-like family protein"
                     /protein_id="HDI6041989.1"
                     /translation="NTTNIATNTTNITNLTDTVNNLGEDALKWDDAAGAFTAAHGTNA
                     TNKISNVQAGIVSSDSTDAINGSQLYGLADSFTSYLGGGADISDTGVLTGPTYSIGGT
                     DYTNVGDALAAINTSFSDSLGDALLWDATANDGAGAFSAGRGVDNTASKITNVANGAI
                     SATSSDAINGSQLYTTNKYIADALGGNAEVNADGTITAPTYTIANTDYNNVGEALDAL
                     DENALLWDATANNGEGAYNASHDGKASIITNVADGNIGEGSTDAINGSQLFNTNMLIQ
                     QNSEVINQLAGNTSETYIEENGAGINYVRTNDTGLTFTDASAQGVGATAVGYNSVASK
                     ASSVAIGQDSRSEVETGIALGSSSVSSRLIVKGSRDTSVSEEGVVIGYDTTDGELLGA
                     LSIGDDGKYRQIINVADGSEAHDAVTVRQLQNAIGAVATTPTKYYHANSTAEDSLAVG
                     EDSLAMGAKTIVNGNAGIGIGLNTLVLADAINGIAIGSNASANHANSIAMGNGSQTTR
                     GAQTNYSAYNMDAPQNSVGEFSVGSEDGQRQITNVAAGSADTDAVNVGQLKVTDAQVS
                     QNTQSITNLNNQVTNLDTRVTNIENGIGDIVTTGSTKYFKTNTDGVDANAQGKDSVAI
                     GSGSIAAADNSVALGTGSVANEENTISVGSSTNQRRITNVAAGVNATDAVNVSQLKSS
                     EAGGVRYDTKADGSIDYSNITLGGGNGGTTRISNVSAGVNNNDAVNYAQLKQSVQETK
                     QYTDQRMVEMDNKLSKTESKLSGGIASAMAMTGLPQAYTPGASMASIGGGTYNGESAV
                     ALGVSMVSANGRWVYKLQGSTNSQGEYSAALGAGIQW"
BASE COUNT         8974 a         8573 c         8958 g         9251 t
ORIGIN      
        1 ggtcgatggt cttttagtgc ggcaacgcct gcgacgatat cggggttttt aaccattttt
       61 attgttaatt catcaccctg cgtggcctgc tttttgattt tttctggggc cacggtagct
      121 gcgcgataat ccgccacggc ggcgcaaccg ataaaaatat tttgctgctg tacagaagca
      181 ttcacggcgg cttccatttc cagcgcggtc atcacatcaa cacggttaac aaacggtggc
      241 gtcggtagtg aaaccggacc tgataccagc gtgacgttcg cgccacgacg ggctgcggcg
      301 gcggcgatag caaaacccat cttgccggag ctgtgattag agatataacg cacgggatcg
      361 agcggttcac gcgtcggacc ggcggtaatc ataatgttca gatgtttcag gtcgttgacg
      421 ggcgaaaaat gcgctaccgc catatccaca atggttaacg gatcgagcat tcgccctggg
      481 ccgatatcac cacaagcctg actgccgctg tctggccccc agatgagcaa accacgcgag
      541 gcaagcacct ctaaattatg ctgggtggcg gcggcacggt acatctgctg gttcatggcg
      601 gggagcacgg ctacaggcgc aggtgtagcc agacaaatcg tcgataccag gtcattcgcc
      661 attccggcag caacacgggc aattaaatct gccgtggcag gggcgagaat cactaaatca
      721 gcccatttac ccagctcaat atggcccata gcggcttcgg ctgccgggtc cagcagactg
      781 tcggaaacgg gataaccaga aaccgcctgc aagctaagtg gggtgataaa ggcttttgcc
      841 gcttcggtca tggctacgcg gacgtcggcc ccgcgatcgc gcaaacgacg caccagttca
      901 ggggttttat aggcagcaat accgccgctg acgccgagaa cgattttttt accggccagg
      961 ctcatgatga tttttcctgt tgggtgacac cagaagttgg cgattttatc acaatacttt
     1021 tgttgtcgtg ccttcacctg agaatcactt tgcgaggcgc tttccaggat tgaaaactgg
     1081 ccgtcgattt aacggaacgg ctatgacagg atgcgagcac cacaaaggag gtgaaggtga
     1141 aaaacaatgc acagctgttg atgccgcgcg aaaaaatgct gaagtttggt attagcgcct
     1201 taacggatgt cgagctgctg gcgctatttc tgcgtaccgg aacgcgcggt aaagatgtat
     1261 taaccctggc aaaagagatg ctggagaatt tcggctctct ttatggcttg ttaacctctg
     1321 aatatgaaca gtttagtggc gttcatggaa ttggcgtggc gaaattcgcc cagttaaagg
     1381 ggattgccga actggcgcgg cgttactaca acgtgcggat gcgtgaagaa agccctttac
     1441 tcagcccgga gatgacgcgg gaatttttac aaagccagct tacgggcgag gagtgggaga
     1501 tctttatggt gatctttctc gactcacaac accgggttat aacgcatagc cgtctttttt
     1561 ccggcacgct aaaccatgtt gaagtccatc ctcgggaaat tatccgcgaa gcgataaaaa
     1621 taaatgcctc ggcgctgatc cttgcacata atcacccttc gggttgtgct gaacccagta
     1681 aagcggataa actcattact gaacggataa taaagagttg tcagttcatg gatttacgcg
     1741 tgctcgacca tatcgtgatt gggcgtggag agtatgtttc ttttgccgaa cgcggctgga
     1801 tttaacccgc tatgcgcgat ccttcgggat ctttgtctgt tcgggacttg agcacatcgc
     1861 tgagtcagcg tatactacgc cacctttgag aatctcgggt ttggcatttg ggcctggcaa
     1921 tcgagagttc acatagaact gcgatgaccg ggctgtaaag cctgacgagg cgccaatacc
     1981 ccatacgaag ctcgagctaa tttgattttt ggagaataga catgtcccga gtctgccaag
     2041 ttactggcaa gcgtccggtg accggtaaca accgttccca cgcactgaac gcgactaaac
     2101 gccgtttcct gccgaacctg cactctcacc gtttctgggt tgagagcgag aagcgttttg
     2161 tcaccctgcg cgtatctgct aaaggtatgc gtgtaatcga taaaaaaggc atcgatacag
     2221 ttctggctga actgcgtgcc cgtggcgaaa agtactaagt acttagagga aataaatcat
     2281 ggctaaaggt attcgtgaga aaatcaagct ggtttcttct gctggtactg gtcacttcta
     2341 taccactacg aagaacaaac gtactaagcc ggaaaaactg gaactgaaaa aattcgatcc
     2401 agttgttcgc cagcacgtga tctacaaaga agcgaaaatc aaataatttt tgctttgatg
     2461 taacgaaaaa cctcgccccg gcggggtttt ttgttatctg cctgccccca tattgactgc
     2521 atctgttcat tcctggagat gctatgcctg aattacccga agttgaaacc agccgccgcg
     2581 gcatagaacc gcatctcgtt ggtgcaacca ttcttcatgc ggtggtgcgc aacggacgct
     2641 tgcgctggcc ggtttcagaa gagatctacc gtttaagcga ccaaccagtg cttagcgtgc
     2701 agcggcgggc taaatatctg ctgctggagc tgcctgaggg ctggattatc attcatttgg
     2761 ggatgtctgg cagcctgcgc atccttccag aagaacttcc ccctgaaaag catgaccatg
     2821 ttgatttggt gatgagcaac ggtaaagtgc tgcgctacac cgatccgcgc cgctttggtg
     2881 cctggctgtg gaccaaagag ctggaagggc acaacgtgct ggcccatctt ggaccggagc
     2941 cgcttagcga cgatttcaac ggtgagtacc tgcatcagaa gtgcgcgaag aaaaaaacgg
     3001 cgattaaacc atggctgatg gataacaagc tggtggtagg cgtagggaat atctatgcca
     3061 gcgaatctct gtttgctgcg gggatccatc cggatcggct ggcgtcatca ctgtcgctgg
     3121 cagagtgtga attgttagct cgggtgatta aagcggtgtt gctgcgttcg attgagcagg
     3181 gtggtacaac gctgaaagat tttctgcaaa gtgatggtaa accgggctat ttcgcgcagg
     3241 aattgcaggt ttacgggcga aaaggcgagc cgtgtcgggt gtgcggtacg ccgattgtgg
     3301 cgactaaaca tgcgcagcgg gcaacgtttt attgtcggca gtgccagaag taattgatgc
     3361 gagccggatg gcataccatc cggcataaac gctacgctaa cttcgccatc agcgcctgat
     3421 ggacattctc cggcaggaaa tgggtgacat cgccctgatg gcgcgccacc tctttcacca
     3481 acgatgaaga gataaacgac cactctttcg acggcatcag aaacacactt tccagttccg
     3541 gcattaagtg gcgattcata tgcgccagct gcatttcata ttcaaaatcc gccaccgcac
     3601 gcaggccacg aatcagcacc gtagcgtgtt gattacgggc gaagttcgcc atcaaatcac
     3661 taaacccgac cacttccacg ttccccagat gcgcggttgc ctgctgtgcc agcgccacac
     3721 gctcttccag ggtaaacatc ggttttttac tgggactagc ggcaatcgcc agaataacgt
     3781 gatcgaacat ttgcgtggcg cgcgtcacga tatcgatatg accattggta atgggatcga
     3841 aagtacccgg ataaatcgcc cgtttttgca taacaacctc aatgcgtttt cggtggcagg
     3901 taaggttcca gcagttgaag cagacgctgt agtgcgccct ggttttgata cagcacttca
     3961 acggcatgac ggccatagaa actacggtaa tcggcgtcgg tgagtaaaga ggaaacctct
     4021 tttgcaagcg tagtggcatc ggtaacggta atcagcccgc ttgcctgctc cagccgcgcg
     4081 caaatgtctt taaagttaaa cgtatgcggc cccatcaata ccggaatagc gtgtgcggcc
     4141 gcttccagcg gattatgccc accacgttca accagtgaac cgccaacaaa ggcgagatcg
     4201 gcaatgccgt acagtaacat caactcgccc atcgtatcgc caaccacaac ctgcgtgctg
     4261 gtggagggga cttcccctga agatcgtgtg atatagctta gtccagcctg gcggacaagg
     4321 ttaatcgcat ccgggaagcg ttccggatga cggggtacca ggatgagcaa taaattcggg
     4381 aattgctgta acaatgcctg atgtgcggcg atcaccacac tctcttcgcc ttcgtgagtg
     4441 ctggtggcaa tccataccgg gcggtgtggt gcccactggc ggcgcagcgt cactgcttta
     4501 gcagccagtt gcggcgttac agaaatatcg aatttcaggc taccggtgac ggtcacctga
     4561 ttattttttg cgcccagtga tacaaaacgt gcaccatctt cttcattttg cgcagcaatc
     4621 agcgtaatac gacgcagcaa gcgacggacg aatttaccca gtttggcata acctgcggcc
     4681 gagcgggcag agagtctcgc gttagcgatc accagcggaa ttttacgttt atgtagcgcc
     4741 gcaatcaggt taggccatag ttcggtttcc ataatcaaca ccagtttagg gtcgacttta
     4801 ttcaggaaac gattaagcgc atcgggcagg tcgtacggca gataaacgtg ctgaacatcc
     4861 ttcccgaaag ccgattgtac gcgctccgaa ccggttggcg tcatggttgt aacggtaatc
     4921 ggtaaatcag gataacgatg gcgcagcgcg cgcaccaacg ggatcgccgc cagagtttca
     4981 ccgacggaga cggagtgcag cataatgccg cctggtttta gcggatggcg gtaaaaaccg
     5041 taacgttcac cccagcgttt tcgataggcc ggagccttac gtccgcgcac ccagagccgt
     5101 atccagatca gcggctgaat aaggtagaga agggcggtgt aaagcaattc gagcatagta
     5161 aatagctgac ttatggatgt gctggggatt ctatgtattt agctgtggct ttaccattac
     5221 ttttcccgtt tttgacttaa atagcaattt acccgccaat tcttgctgaa catctatact
     5281 ttttttgtat ttttatgcga ttcattgatt tctgcagtac gtgcaggaag gaatcgcaga
     5341 gcaacagtaa aagaatatgg ttgctaatga ttttatttga ttgatttctt tattgaaaat
     5401 caacaatgtg cttaaaaatc attcgggtcg gattgccgcg aaagaagtga tacactagcg
     5461 cgtcaatatc agtgcggtat cagtattcag gtagctgttg agcctggggc ggtagcgtgc
     5521 tttttctgct tcaccttaat cggataatct caacaaaaag agttacttgt ggataagcca
     5581 tttcgaagaa ttttgctcat taagatgcgt tttcatgggg atatgttatt aactactccc
     5641 gtcattagtt cgctgaaaaa aaattaccct gacgcaaaaa tcgatgtgct gctttatcag
     5701 gacaccatcc cgatcctgtc tgaaaatcca gagattaacg cgctctacgg cataaaaaat
     5761 aaaaaagcaa aagcctcaga aaaaattgcc aactttttcc atctcatcaa gatattacgt
     5821 gccaataagt atgaccttat cgttaatctt accgatcaat ggatggttgc tatactggtt
     5881 cgcttattaa atgcccgtgt gaaaatttcc caggattatc atcatcggca gtctgctttt
     5941 tggcgtaata gtttcactca tttggtgccg ttgcagggtg gaaatgtggt ggaaagtaac
     6001 ttatccgtgc tgacgccatt gggacttgaa tcgttggtga agcagacaac catgagttac
     6061 ccgcctgcaa gctggaaacg tatgcgtcgc gaacttgatc acgctggtgt tggacaaaat
     6121 tatgtggtta tccaacctac ggcgcggcaa atcttcaaat gctgggacaa cgccaagttt
     6181 tccgctgtga ttgatgcctt acatgctcgt ggttatgaag tcgttctgac gtccggccca
     6241 gataaagacg atctggcctg cgtcaatgaa attgcgcagg gatgccagac gccaccagta
     6301 acggcgctgg ccggaaaggt gaccttcccg gaacttggtg cgttaatcga tcatgcgcag
     6361 ctgtttattg gcgttgattc cgcaccggcg catattgccg ctgcagttaa tacgccgctg
     6421 atatcgctgt ttggtgcgac agaccatatt ttctggcgtc cctggtcaaa taacatgatt
     6481 caattctggg cgggagatta ccgggaaatg ccaacgcgcg atcagcgtga ccgaaatgag
     6541 atgtatcttt cggttattcc ggcggcagat gtcattgcag ctgtcgataa attacttccc
     6601 tcctccacga caggtacgtc gttatgatcg ttgctttttg tttatataaa tattttccct
     6661 ttggcggttt gcagcgcgat tttatgcgta ttgctcagac agttgccgcc cgaggtcatc
     6721 atgttcgggt ttatacccag tcgtgggaag gcgaatgccc tgatgtattt gaactgatca
     6781 aagtgccggt taattcgcat accaatcacg ggcgcaatgc ggaatatttt gcctgggtgc
     6841 aaaaacattt acgtgaacac cccgtcgata gagtcgttgg atttaataaa atgccggggc
     6901 tggacgttta ttatgccgct gatgtttgtt atgccgagaa agtagcgcag gagaaaggct
     6961 ttttctatcg cctgacgtca cgttatcgcc attatgccgc ctttgagcgg gcaaccttcg
     7021 aacagggcaa gccgacacag ctgctgatgc tgacagataa gcaaatcgcc gatttccaga
     7081 aacattatca gactgaagcg gaacgttttc atattctgcc accggggatt tatcctgatc
     7141 gtaaatatag ccagcaacca gcgaatagcc gtgaaatctt ccgtaagaag aatggaataa
     7201 ccgaacaaca atatttattg ttgcaggtcg gttcagactt cacgcgtaaa ggtgtcgatc
     7261 gttccattga agcacttgct tcgttaccgg attcgctgcg ccacaacaca ttactatatg
     7321 ttgttgggca ggataaaccg cgaaaatttg aggcgctggc agaaaaacgc ggcgtgcgca
     7381 gtaatgttca cttcttctcg gggcgcaacg atgtctcgga attaatggcg gcggcggatt
     7441 tattacttca tcctgcctac caggaagcgg cgggaattgt gttgctggaa gcgataactg
     7501 caggattacc ggtactaaca acagccgttt gtggctatgc gcattatatt gtcgacgcca
     7561 attgcggcga ggctattgct gagccattcc gccaggaaac attgaatgag attttacgca
     7621 aagcgttaac gcaatcttca ttgcgccagg cctgggcgga aaatgcgcga cattatgctg
     7681 atacacaaga tttatacagt ctgccagaga aagcggcgga catcataacg ggtggtctgg
     7741 atggttgaac ttaaagagcc gtttgccacg ttatggcgcg gtaaagatcc ttttgaggaa
     7801 gttaaaacct tgcagggtga ggtatttcgt gaactggaaa ctcgccgcac tctgcgcttt
     7861 gaaatggcgg gcaaaagcta ttttctcaaa tggcatcgcg gcacgaccct gaaagagata
     7921 atcaaaaatt tactctcatt gcggatgcca gtattaggcg cagaccgcga atggaatgcg
     7981 attcatcgac tgcgggatgt cggcgttgat actatgtatg gggtggcatt cggcgaaaaa
     8041 ggcattaatc cgctgaccag aacctcgttt attattaccg aagatctgac accaaccatc
     8101 agtctggaag attactgtgc tgactgggcg accaacccac cagatgttcg cgtaaagcgt
     8161 atgcttatta agcgtgtcgc gacgatggtg cgcgatatgc atgctgcggg cattaaccac
     8221 cgtgactgtt atatctgtca tttcctgctg cacttgcctt tttccggtaa ggaagaggag
     8281 ttaaaaattt cagtaattga cctgcaccgg gcgcagcttc gcacgcgcgt tccatgtcgt
     8341 tggcgcgata aagatcttat tggactttat ttttcttcga tgaatattgg cctgactcaa
     8401 cgggatatct ggcggtttat gaaagtgtat tttgtcgccc cgcttaaaga cattctcaag
     8461 caggaacaag ggctgctgtc gcaagcagaa gaaaaagcca caaaaatcag ggaaagaacg
     8521 attcgaaaat cgttgtaatt tccggagaga aatatgagtg cccactattt taatccacaa
     8581 gagatgatca ataagacaat catcttcgat gaaaggccag aggcgtcagt ggcatcatca
     8641 ttccatgttg cttatggcat tgatcagaac tttctttttg gttgtggtgt ttcaatcacg
     8701 tcagttttgt tacataacaa cgacgtgagt tttgtcttcc acgtttttat tgatgatatc
     8761 cctgacgccg atatccagcg tttatcccaa ttggcgaaaa gctatcatac ctgtatccag
     8821 atccatctgg taaattgtga acggcttaag gtattaccga cgaccaaaaa ttggtctatt
     8881 gccatgtatt tccgttttgt aattgcagat tactttattg atcaacaaga taagatcctg
     8941 tacctggatg ctgatatcgc ctgtcaggga accttaaaac cgctgataac aatggatctt
     9001 gccaataacg ttgctgctgt tgttactgaa cgcgatgcta actggtggtc gttacgggct
     9061 caaagtctgc agtgtaatga acttgaaaag ggttacttta attcaggtgt cctgttaatt
     9121 aatacacctg cgtgggcgca ggagtccgtt tctgctaaag cgatgtcgat gcttgctgat
     9181 aaagccgtcg tttcccgttt aacctatatg gatcaagata tactaaatct tatcctgtta
     9241 gggaaagtta aattcattga tgctaaatac aatacgcaat ttagtttaaa ttatgaatta
     9301 aaaaaatcat ttatttgtcc aattaatgat gaagccgtat taattcatta tgtcggcccg
     9361 acaaaaccct ggcattactg ggccggttat ccaagcgcgc agccatttat caaagccaaa
     9421 gaagcatcgc cctggaaaaa tgaacagtta atgcggccaa ttaactcaaa ttatgctcgt
     9481 tattgcgcca agcataattt taaacaaaat aaaccaatta acgggataat gaattatatt
     9541 tattattttt atttaaagat aataaaatga cattgagaaa agttaaatga atgaatttat
     9601 aaaagaacgg ttttcgtatt tagcagataa taaaaaagaa aacgtcccag agctaaatgt
     9661 ttcctacggt atcgataaga attttttgta tggtgctggc gtttcaattt cttccgtttt
     9721 gattaataat tcagatatta attttgtctt tcatgttttc actgattatg tggatgatga
     9781 ttatttaaag tcatttaatg aaacagcaaa acaatttaat acctcaatta ttgtatattt
     9841 aattgaccca aaatactttg ctgatctgcc gacgtcacag ttttggtcgt acgcgacata
     9901 cttcagggta ttgtcctttg aatatctgag tgaaagtatt tccacactgc tgtatctgga
     9961 tgccgatgtc gtttgtaaag gaagtctgaa acctctcaca aaaattatat ttaaagatga
    10021 gtttgctgcg gttattcctg acaatgatag tactcaggcg gcatgtgcaa aacgtcttaa
    10081 cattcccgaa atgaatggac gttatttcaa tgcaggcgtt atctatgtca atcttaaaaa
    10141 atggcatgaa gcaaatttga caccgtattt actcacgctt ttacgagggg aaactaaata
    10201 tggctctctt aaatatttag atcaggatgc gttgaatatc gcatttaata tgaataatat
    10261 ctacctcgcg aaggattttg atactattta taccctgaaa aacgaacttc atgatcgtag
    10321 tcatcgaaag tatcagcaaa ccattaccga taaaacagta ttgattcact atacagggat
    10381 aactaaacca tggcatagct gggctggata tccgtctgca tcatacttta atatcgcgcg
    10441 tgaacaatct ccctggaaga aatatcctct taaagaggcg cggactgttg cagaaatgca
    10501 gaaacaatat aagcatctgt ttgcccatgg tgagtatata aaaggcataa cttcattaat
    10561 taagtacaag cttaagaaat aaattactct tatgattaca agtatacgtt accgcggctt
    10621 ctcattttat tacaaaaata atgataataa atataaagaa atctttgatg aaatactggc
    10681 ctataatttc aaaacagtaa aagtattacg taatattgat gataccaaag tatcgttgat
    10741 tgatacgaaa tatggtcgct atgtttttaa agtctttgct ccgaaaacga aaaggaatga
    10801 acgtttttta aaatcttttg taaaaggtga ctactatcag aatttgatcg ttgaaaccga
    10861 tcgtgtcaga agtgcaggtc ttacttttcc taatgatttt tattttctgg ctgagcgtaa
    10921 aattttcaat tatgccagtg tttttatcat gcttatcgag tatgtcgagg gcgtagagct
    10981 caatgatatg ccgattattc cagaagatgt taaagcagaa attaaggctt caatggaaaa
    11041 attgcatgcc ttaaatatgc tatcgggcga cccacaccgg ggtaacttta tcgttagtaa
    11101 agatggtgtc aggataattg acctatccgg taaaagctgt acggcagagc gtaaagctcg
    11161 tgatcgtctg gccatggaac gtcacctggg tattgccaac gaaataaaag attatggcta
    11221 ctattcagta atctacagaa ccaaactacg caaatttatt aaaaaattaa aaggcaaagc
    11281 gtaaaccaca cagtcaaaac ggaaccaaca tggatttatt agctgagagt attactgaag
    11341 tcgctgtctc tggggaaatt gctaacaccg atcgtgtgtt aaatatcgct tacggtattg
    11401 accgcaactt tttatttggt gcagcagtat ctatgcaatc agttgttatg cataacccgg
    11461 atcttgcggt taagtttcat ctctttactg actacattga tgaagactat ctacaacgtg
    11521 ttaatgcttt taccagcaaa aatgctaacg ttgaagtaag aatttataaa gtatccagtg
    11581 cctttattga tatcttcccc agcctgaaac agtggtctta tgcaacattc ttccgtttag
    11641 ttgcgttcca gtatctgagt gaaactattg aaaatctgtt atatatcgat gctgatgtca
    11701 tctgtaaagg ctcattagct ggattgcttg atattaattt tgatggcgat aaattcgcag
    11761 ctgttattaa agatgtgcct tttatgcagg aaaaaccagc gaagcgtctg gctatagagg
    11821 ggcttccagg gaattatttc aacgccggtg tagtatatct gcagcttgaa gcgtgggcga
    11881 aaaatgattt tatgaataaa gccattgcta tgctggcaag tgacccgcag cacacgaagt
    11941 ataaatgcct tgatcaggat attttaaata ttctgttctt tggtcattgt atttttatta
    12001 gcggcgatta tgattgcttt tatggcattg actatgagtt aaaaaataaa agcgatgaag
    12061 attataaaaa gaccattacc gatgatacta agctgattca ttatgttggc gtaacgaagc
    12121 cctggaacga ctggacgaat tatccctgcc agaagtattt taatgaggct tatcaggctt
    12181 cttgctggaa tgatgtggcg tttattccag ccacgaatga aaagcagtat caagtgaaat
    12241 accaacatgc aaagaaaaat ggtgatacgt ttaacgcttt tatttacttc attaaattca
    12301 aattaaataa gtataaaaga aaactattta ggcaataata aaaattatgg atgtctaagt
    12361 tacaataata attaaggatg agtcctatca tattttatta gttcaggcaa gagaacacga
    12421 tgagcaatga ttacccttta gtatccataa taataccgac gtataattca tctgattaca
    12481 ttactgaaac tctaacgaaa ttagaaaaac aaacttaccc aaattttgaa attgttattg
    12541 ttaatgatgg ttctaaagat aacacatcaa atgttttgag agagtatggg ttaacccact
    12601 ctcgattaat tattctcaat aaagaaaatg gcggtgtttc gtctgccagg aatacaggta
    12661 tccgcaaggc gcaaggacag tttatatgtt ttatggatga tgatgatgag atagatccta
    12721 actatctgct gaagatgtat tccagacaac atgagacggg aggggatgcg atttattgtg
    12781 ggctttatgg acatcatata aaaaatggtg ttacttactc acctataaat acagatttta
    12841 atgaagggtc tttacttttc gatttttttt ataaaaaggt cagattccat atagggtgct
    12901 tgtttataag aaaacaactc ctggaagata ataatctttt ttttgatgaa gatttacgac
    12961 taggagaaga tctggatttt atctatcgac tgctaattac atgcgatatg tatgcggttc
    13021 catattatat gtataagcat aactatagag aaaattccct aatgaactca tgtagaacca
    13081 tcactcatta tcgacatgag tcatttgcgc acgaaagaat ctactcttct gtgatgcagt
    13141 tatacaaagg taaccggaaa gaagaaattc atacattatt gagtaaaaat agaacttatc
    13201 ataaaactcg ttatttatgg aatgttctac ttaatggcga ttttgaatta ttaaatcaat
    13261 tagttgaaag caatgaaaaa gaattgaaag attgtaatct tcttggcaag agagataaga
    13321 ggcgagcaaa aatattagca tcaaaaaact atattctctg gaggatggta agactggtaa
    13381 atagaaaaaa gaataaacgt taggtcttgc attaaataac ccatcctcgt agcataggtt
    13441 gaaattatat gtcgttttgt tggaaagaaa ttaactctgg tgtcaagtct ttaattctca
    13501 tattatgtat gttgtcttta atgactttgt ctttgtggga tgatgttgca acaaagtttc
    13561 ttcatgcagc tggaattata tctacattat attttctagc gacagcaaaa aaaacaataa
    13621 ctaataatcc tactttgtta atttttatct cattatgtct tttgggtatc gttaatatca
    13681 tctggtattc acattataaa gtttcaggct cggtttatac caatgcatat cgtggcccaa
    13741 tggaaactgg aaaaattgcc ttgtgtagtg cttttatttt cttagttctt tttgctaaag
    13801 atgagatgag aacaaaaata aaatttggga aactaattct gttcgcatcc ctggcaacgc
    13861 agttactttt ttttgcgcat gccatgtggc aacatttcta tttaaacgta gaccgtgttg
    13921 cattatcagc ttcccacgct acaacagcag gctacatcat ccttttccct tctttactgg
    13981 catcaattct cattttaaaa tgcgacttta gacataaaac aacattatat acaattaact
    14041 tcatgcttag cttatgtgct gtcatagtaa ctgaaacgcg tgcagccata ttagtgtttc
    14101 cattctttgc gttaatatta atcgtaatgg atagttatat taataagcga attaattata
    14161 agttatattg ttttattgcg attgcattat tagcaggtgt gttttctttt aaagatacat
    14221 tgcttatgag aatgaatgac ttaaataacg atttagttaa ttattcgcat gataacacca
    14281 gaacttcagt cggtgcccgt ctggcaatgt atgaagttgg tttaaaaaca tattctccaa
    14341 taggacaatc actggaaaaa cgcgcggaaa aaatacatga actagaagag aaagagccta
    14401 gattaagtgg cgctttaccc tttgtagatt ctcatttgca taacgatctc atagatacgt
    14461 tatcaacgcg tggtattcct ggagtagtat taacaatttt agccttttca gcaatattca
    14521 tatatgcctt aagaactgct aaagaacctt atattttaat cttgcttttt tcactactgg
    14581 tagtaggcct aagtgatgta atactctttt ctaaaccggt tccgactgct gtgtttgtca
    14641 ccataatatt gctttgtgct tattttaaag cacaatcgga ccaatgttta ttagataagt
    14701 aatgaatatg tgaaataaaa tcagcaagga agctgatttt atttcaaaat tatctattaa
    14761 agagttgtaa tttaaatcat ggcagcgttt tcttcaatga accgcttaac agcatttgct
    14821 gtcaattgag acaactcatt ccccggagcc ctacaaacca tctgattctt cccataccca
    14881 ccaattagtc ccggatcggt aggtccataa accgtgatat tgggtctatc cagtgccgcc
    14941 gttaaatggc ttaaccccgt atccaccgac actacaaatt tagccccagc cagtacgcgg
    15001 gcaacgcctt ccagactcat cttcggcaat acttcaacat aagcaaatcc ttccgccagt
    15061 cgtttcgccc gttcttcctc atgcggcgcg ccccacggaa gtttaatccg tattcctgaa
    15121 tcagccagta aaccaatcaa ttctcgccag tgttcttccg gccagtgttt atcatctcgg
    15181 gttgttgcat gaagaaatac ggcatattcg ccagcatctg taggcaggtt cgtcaggaaa
    15241 tgctgtgcga tagcataatc gccctgggtt tgtggtttgc tatagcccag acttttggca
    15301 aacagttcgc gggtgcgttc tacggcgtgc tgctgttttg caatatgatg tttacgattg
    15361 taaaacaggc tggctaacgg ttcgcgagcg gtttgccagt ccattccatg ctttacgcca
    15421 tgcgccagac gcgtcaccag cgctgcgctt tttaccagcc cctgagcgtc gataactgcg
    15481 tcataattct ctgcttgtag cgtttcacga aacgctttgc gttcagcttt tatgggggcc
    15541 gagaaccagg ctttacgcca gcgacgtatt gccacaggaa taactcgctc aacggcggcg
    15601 tgccaggaag gaatctgtgt gaacccttct tccaccaccc agtcaaactt aatccctggg
    15661 attgcctgct gggcatcagt gagtgcgggc agcgtatgga gaacatcgcc catcgacgat
    15721 gttttaacga tcagaacccg catccgtcag gcttcctctt gtaacaatag cgcgttgagt
    15781 tcttccagta cgcgctgggg agtaatgtcg atcaagctct ggtgataacc ctccgctgcg
    15841 tcacctttac gcactttgtg atagccggta atcaggcgga tcacgcgtgc tttatgggat
    15901 agcggcggtg tgaagtccgg gctactcgga ccatacaagg caaccagcgg acgattgagc
    15961 gccgccgcaa cgtgcatcag gccagaatcg ttagtgacaa tggctttaca ggctgcaatc
    16021 aggataaccg cttgatcaag ctgtgtttcc cctgccaggt tccggcacca tgcctgctgc
    16081 tcagtattta aagcggcaag aatctcattg cccgcttcat gatctttcgc agagccaaac
    16141 agaaccacct gataaccttc atcaatcagc tgctttgcca gctccgcata gtggtagtgt
    16201 ggccagcgtt ttgccggacc aaactccgct ccggggcaaa agccaatcat cggacgttct
    16261 gatgagagcg aaaattgatt acaggtatat gatttttcac cttcgctcac ctgcaactgc
    16321 ggccataaca atggctgcgg cagatcttgt gctgtacgca taatgccttt gtcataggcc
    16381 agcgccacat agcgttccac cattagcggc caggcttctt tatcgagcac gcgtacatcg
    16441 ttaagtaaac cgtagcgcat ctcgccgcgc cagccggtgc gatgaggaat acccgcgaag
    16501 aaaggcacta atgcggattt gaaggagttg ggtaagacgt aggcgcggtc gtagcgcttt
    16561 tcacgcagac tatgacccag tttgcggcgt tcgccgattt ccagcgctcc gtgaccgaga
    16621 ggcataggga tcgcttcgtt aacttccggc atccgcgata ataatggacg gcaccatgcc
    16681 ggtgccatca cgtcgattat cgcctgggga tagcgcgcct ggagcgtgcg atagagactt
    16741 tgcgacatca tcatgtcgcc aacccaagac gggccgatca ccagtatttt catgcagagc
    16801 tcttatgcgt cacgattcag ccaggccatg tattccgtta cgccttcggc aacggttttg
    16861 aacggtttat cgtaacccgc cgcgcgcaga ttcgtcagat ctgcctgcgt gaacgcctgg
    16921 tagcggcctt tcagtttatc cgggaacgga atgtattcga tttggccttt cttgtgataa
    16981 gcaagtgtag catctgctac tgcctggaag gattccgcac gaccggtgcc gaggttgaag
    17041 atgccggaaa cgccattttc caggaaccac aggtttacat ctgccacatc gcccacatag
    17101 acgaagtcac gtttgaagtt ctcgctacct tcgaacagct tcggtgattc accgttatta
    17161 agctgggtgt tgagatggaa agcgacgctc gccatgctgc ctttatggcc ttcacgcggt
    17221 ccataaacgt tgaaataacg gaagccaaca atctgcgagt tggcttccgg caggatttgg
    17281 cgaacatatt catcaaacag gaacttcgaa taaccgtaga cgttaagtgg tttttcgtac
    17341 tcgcgggatt caataaagtc ggaagtgcgt ccgccgtaag tggctgcaga agaagcgtac
    17401 agcaacggga tttcgcgctc caggcagtag tgcagcagct ctttggagta ttgatagttg
    17461 ttatccatca tatacttgcc gtcccactcg gtggtggaag agcacgcgcc ttcgtggaaa
    17521 atcgcttcga catcgccgaa ctcttcgcca gccataatct ggatcaggaa gtcttcctta
    17581 tccatatagt ccgcgatatc cagatccacc aggttcacaa acttggtgcc gtctttcagg
    17641 ttatccacca ccagaatatc ggtgatgcct ttgtcattca gggctttaac gatgttgctg
    17701 ccgataaagc ccgcgccgcc ggtaacgatg atcataactg taaccttcga attatggagt
    17761 cagagacaat ctcagacacg aatgcttcta tcatatcact acacggcgtt ggcttcagcc
    17821 attcgccaaa agtgagattg atggacgtta tttatcctga attttgcaga agtgttaatg
    17881 cgtcatctcg tcgcgaccta taagtttggg taatatgtgc tggaatttgc cctgtctgga
    17941 gaatcgcaat gcgtggagaa ttttatcagc agttaaccaa cgatctggaa accgcacggg
    18001 cggaagggtt gtttaaagaa gagcgcatta ttacgtctgc gcagcaagca gatatcactg
    18061 tggctgatgg aagccacgtc attaactttt gtgccaataa ctatctcgga ctggcgaatc
    18121 atccggatct gattgcggcg gcaaaggcgg gaatggattc tcacggtttc ggcatggctt
    18181 cggtgcgttt tatttgcggc actcaggaca gtcataaaga gcttgaacaa aaactggcgg
    18241 ccttcctggg gatggaagat gcgattctct actcttcctg ctttgatgct aacggtggcc
    18301 tgttcgaaac gctgttaggc gcggaagacg ccattatctc cgacgcactg aaccacgcgt
    18361 ctattattga tggtgtgcgt ctgtgtaaag ctaaacgcta tcgttatgcc aacaacgata
    18421 tgcaggagct ggaagcacgt ctgaaagaag cgcgtgaagc cggtgcgcgt catgtgctga
    18481 ttgccaccga tggtgtgttc tcgatggacg gcgtgattgc caacctgaaa ggtgtttgcg
    18541 atctggcaga taaatacgat gccctggtga tggtagacga ctcccacgcg gtcggttttg
    18601 tcggtgaaaa cggtcgtggt tcccatgaat actgcgaagt gatgggccgg gtcgatatta
    18661 tcaccggcac gctcggtaaa gcgctgggcg gggcttctgg tggttatacc gcggcgcgca
    18721 aagaagtggt tgagtggctg cgccagcgtt ctcgtccgta cctgttctcc aactcgctgg
    18781 caccggccat tgttgccgct tccatcaaag tgctggagat ggttgaagcg ggtagcgagc
    18841 tgcgtgaccg tctgtgggcg aacgcgcgtc agttccgtga gcaaatgtcg gcggcgggct
    18901 ttaccctggc gggagccgat cacgccatta ttccggtcat gcttggcgat gcggtagtag
    18961 cgcagaaatt tgctcgtgag ctgcaaaaag aggggattta cgtcaccggt ttcttctatc
    19021 cggtcgttcc gaaaggtcag gcgcgtattc gtacccagat gtctgcggcg catacccctg
    19081 agcaaattac gcgtgcagta gaagcattca cgcgtattgg taaacaactg ggcgttatcg
    19141 cctgaggatg tgagatgaaa gcgttatcca aactgaaagc ggaagagggc atctggatga
    19201 ccgacgttcc tgtaccagaa ctcgggcata acgatctgct gattaaaatc cgtaaaacag
    19261 ccatctgcgg gactgacgtt cacatctata actgggatga gtggtcgcaa aaaaccatcc
    19321 cggtgccgat ggtcgtgggc catgaatatg tcggtgaagt ggtaggtatt ggtcaggaag
    19381 tgaaaggctt caagattggc gatcgcgttt ctggcgaagg ccatatcacc tgcggtcact
    19441 gtcgcaactg tcgtggcggt cgtacccatc tgtgccgcaa cacgatcggc gtcggcgtta
    19501 accgtccggg ctgctttgcc gagtatctgg tgattccggc gttcaacgct ttcaaaatcc
    19561 ccgacaatat ctccgatgat ctggcttcca tttttgatcc cttcggtaac gccgtgcata
    19621 ccgcgctgtc gtttgatctg gtgggcgagg atgtgctggt ctctggtgca ggcccgattg
    19681 gtattatggc ggcggcggtg gcgaaacacg ttggtgcacg taacgtggtg attactgatg
    19741 ttaacgaata ccgccttgag ctggcgcgta aaatgggtat cacccgtgcg gttaacgtcg
    19801 cgaaagaaaa ccttaatgat gtgatgacag aactgggcat gaccgaaggc tttgatgtcg
    19861 gtctggaaat gtccggtgcg ccgccagcgt ttcgtaccat gctcgacacc atgaaccacg
    19921 gcggtcgtat tgcgatgctg ggtattccac cgtccgatat gtctatcgac tggaccaaag
    19981 taatctttaa aggcttgttc attaaaggta tttacggtcg tgagatgttt gaaacctggt
    20041 acaagatggc ggcgctgatt cagtctggcc tcgatctctc gccgatcatt acccatcgtt
    20101 tctctatcga tgatttccag aagggctttg acgctatgcg ttcgggccag tccgggaaag
    20161 ttattctgag ctgggattaa cacgaacaag ggctggtatt ccagcccttt tgcctgagga
    20221 aaatccgtta aatatgtaaa atcctgtcag tgtaatcaag atatcgtaat tgcgctgatc
    20281 tcttatatag ctgctctcat tatctctcta ccctgaagtg actctctcac cggtaaaaat
    20341 aatatctcac aggcttaata gtttcttaat acaaagcctg taaaacgtca gggtaacttc
    20401 agagatcgtt ggtaatttat gatgaacagc actaataaac taagtgttat tatcccgtta
    20461 tataatgcgg gcgatgattt cagcgcttgc atggaatctt taatcgcgca aacctggact
    20521 gctctggaaa tcattattat taacgatggt tcaacggata attctgttga aatagcaaag
    20581 cattacgcag aaaactatcc gcacgttcgt ttgttgcatc aggcgaatgc tggcgcatcg
    20641 gtggcgcgta atcgtgggat cgaggtggcg acgggcaaat atgtcgcttt tgtcgatgct
    20701 gacgatgagg tgtatcccac catgtacgaa acgctgatga ccatggcgtt agaggacgac
    20761 ctcgacgtgg cgcagtgcaa cgctgattgg tgttttcgtg aaacgggtga aacctggcaa
    20821 tccatcccta ccgatcgcct tcgctcaacc ggtgtattaa ccggtccgga ctggctgcgg
    20881 atggggcttt cttcccgccg ttggacacac gtggtctgga tgggggttta tcgccgcgat
    20941 gttattgtta aaaataacat taaatttatt gccggattac atcatcagga tattgtctgg
    21001 acaacagaat ttatgtttaa cgcgctgcgt gcgcgatata ccgagcaatc attatataaa
    21061 tattatctgc ataatacgtc agtgagtcgg ttgaacagac aagggaacaa aaaccttaat
    21121 tatcaacgtc actatattaa gattacccgc ctgctggaga aattaaatcg aaattatgcc
    21181 gacaaaatta cgatttatcc ggaatttcat cagcaaataa cctacgaagc attgcgtgtt
    21241 tgccatgcgg tgcgcaaaga gccggatatt attacccgcc aacggatgat tgccgagata
    21301 tttacttccg gtatgtataa gcgcctgatt accaatgtgc gcagcgtgaa ggtgggttat
    21361 caggcgttac tgtggtcttt ccgcttatgg caatggcgcg acaaaacgcg gtcgcaccat
    21421 cgcattacgc gtagcgcctt taatttgcgc tagcgttaag cttcgccgct tgaggctgtt
    21481 tgccccagcc ttgccactga tgctggaagt aaacgatcag cgtgctctgg ctgatgcttt
    21541 cgcttaatac ttcaaagaag cgattagcat aaacaggttc cagcggcttc ttcggtttgc
    21601 acagcttcac gccacggaat ggattacgcg gtgtgtcggg cacagtgttt ttcggcggtg
    21661 tagacgtatc aacctgcggt tcattcaaca agctgctggc tttcaccagc gtaatgtctg
    21721 gcggcaggtt ataaaccatc tgttgtagca cgcgtaccgt tgaagggtga ggatgtccaa
    21781 tggcaatggt tgaaccgttg cgacgcgcca ggtcaattgc gcgattgaat tgcacacgga
    21841 tgtccgcttc attttgcgaa tcgtcgagga acaccttccg tttgatcacc ttcacgccag
    21901 tgccttgcgc ggcgcgcatc gcctgggtat taccgatggt tacgctgtcg aggaagtaaa
    21961 gattgtaacg ctccagcgcc tgcatcactt tctgcatacc aaacaggtta gaggtcatct
    22021 tgctgcccat gtggttgttg atccccacgg catagggcac gttattgacc gcactacgaa
    22081 taatgcgctc aatttcgtcg ctgctcatct ccgggcgtag cgtatttttc tccagcggct
    22141 gtttactcag tggtgccatc ggtagatgaa tcaacacttc gtgaccgctg ttatgcgctt
    22201 tggtcgccat ctctctggcg tgcggtgaat cgggtaatac agcgacggag atagcggaag
    22261 gcatcgccag cacctggttt tcgttgtgcg ggcgataccc aaaatcatca atgacgatgg
    22321 caagtttgcc agcaattacg ggggaggaga gcgccaacag agcggcaaat gcaagaacgt
    22381 tacgacgaaa tggaaacaaa acttatcttc ccaaccacgg ctgtggattg accgcctgac
    22441 cctggcggcg aatttcgaaa tagagtgaag gccgaccctg accgccactg ctgcccacca
    22501 gtgcaattgg ctggcccgcg cgaacctgcg aaccaacgct caccagtgcg ctctgattat
    22561 agccgtaaag actcatgtcg cctttaccgt gctcaaccac caccaccaga ccgtagcctt
    22621 gcagccagtc agccagaatc acccggccgt cggcaatcgc tttaacttca gtaccttcag
    22681 aggcaccaat caccatccct ttccagcgta attcaccctg tagctgttcg ccatagcgat
    22741 gcagcgttgg cccgcgaact ggccagaatg cctgaccgcg cggtgcgccc agaccaccgg
    22801 tacgggacat cagcgatttt tcgctttcgg tcggcttgta ggtggtgcct ttgcgcgtcg
    22861 cttctttctg gcggtcgcga accgcctggg cttcgcgcgc ttcacgttca gcacgcgctt
    22921 tcgccgcagc ttccgcacgg gcaatgctgt tacgcaggcg ggattcgttg gcgcgcagct
    22981 cgctcaactg ttgctgacct tgctggatgg aagactccag ccctgccagc gtctttttac
    23041 gctcgctcaa cgcctgggtc agcttcgcct gttgggcgcg ctgctcatat aaaagcgttt
    23101 gttgctcgct ctgtttctct tccagttcgg cacgctgcat agcgacttct tcacgcgttt
    23161 gtttcaactg agcaatggtt tcttgtcgcg cctggttgag atagccgaaa taagcctgta
    23221 accgctgccc acgctggctt tcttcaccgc tgagaatcag ctgaataccg gtatgttcac
    23281 cctgacgaaa cgcggcatcc agttgcgcgg cgaggctgcg ctcctgggcg gctttttgct
    23341 gctccagttt ggcaatcgac gcgttcatct catcaatctg tttattcagt tgattaagcg
    23401 tgttttgcgt ttcgcgcagt ttacgggtgg cttcagagat cgcttcttcc tgctttttca
    23461 attgtgcgag caggcttgcg cgttgttgtt gcttttggcg taccgcgcgc tctttcgcgg
    23521 cgatgtcagc ctgaatggat ttgagttggt cacgctcatc cgcgtgggcg gaaaaggcgc
    23581 acaacaatac gccagcgctc agaacgctgg cgtagatgat gggcctgatt gcaaacctgc
    23641 gcggtttcac ggcccgtgtc atggtattaa tcgcctttcc cctcatgggg agggattatt
    23701 ccacgatgaa cagcggctta ccagtcatct cttgcgggat ttccataccc atcagcgaca
    23761 acatggtcgg cgcgatgtca gaaagtttgc cgccttcaac cgctttcacg ttcttatcac
    23821 caacgtaaat cagcggaact ggcaggttgg tgtgtgctgt gtgcgcctga cccgttgccg
    23881 gatcgcgcat ctgttcagcg ttaccgtggt cagcggtgat cagcagttgt ccacccacgg
    23941 actcaaccgc tttcgcgact tcttccacgc agtgatccag cgcttcaacc gctttaaccg
    24001 ccgcttccat caccccggtg tgacctacca tgtcgccgtt cggatagtta cagataatgg
    24061 tgtcgtattt gccgctcttg attgccgcaa ccagtttttc ggtcagctct gcggagctca
    24121 tttccggttg cagatcgtag gtagccactt tcggtgagtt gatcagaatg cgatcttcgc
    24181 ctttgaacga ctcttctacg ccaccgttga agaagaaagt aacgtgggca tatttttcgg
    24241 tttcggaaat acgcagctga gttttgtcgt ttttcgccat ccactcgccg aaggtgttaa
    24301 ccagggaagc gggtgggtaa gcaaccgcag ttttgatgtc ggcagcgtat tcggtcagca
    24361 tcacgaaatc gacgttaacc actttcttac gcgcgaagcc gtcaaaatcg gcatttacga
    24421 atgcacgagt gatttcacgc gcgcggtcag cacggaagtt catgaaaatc agcgcatcgc
    24481 cgtcttccat tgccgcatct ggctgacctt ctgcacgaat aacggtcgct ttcacgaact
    24541 cgtcgttttc gtcgcgagca taagcagcct gcaaaccggc aacggcggta tcggcctgga
    24601 actcaccttg tgccagagtc agcaggtcat aagctttttc aacgcgatcc caacggttat
    24661 cgcggtccat cgcgtagtaa cgaccaatga tggacgctac gcggccttta cccagcgcgg
    24721 caaatttctc ttcgaatttt ttcagcgagg attcagcact gcgcggcgga gtgtcgcgac
    24781 cgtcaaggaa tgcgtgcagg tagatttttt ctgcgccgcg ttcagcggcc agttctacca
    24841 tcgccatgat gtgatcttcg tggctgtgta cgccgcctgc ggagagcaga cccataatgt
    24901 gtactgcttt acctgcgttt ttcgctttat ctaccgcgcc agtcagcact ggattagcaa
    24961 agaaagcgcg atctttgatt tcaacgtcca gacgagtcag gtcctgatac acgatgcggc
    25021 cggcacccag gttaacgtga cctacttcgg agttacccat ctgacggtca ggcagaccga
    25081 cttccagacc ggaagcgtcg atcagggtat gcggacgatt ggcccacagt gcatccatta
    25141 ccggggtttt agcactaaaa atggcgttat cctgctgttc ttcgcgatag ccatagccat
    25201 ccagaatcac cagtaccata ggttttttag aaaccgacat tgcgacaacc tcatactcaa
    25261 gagtcaaaat ttgcgtaatt ttactacagc tgaatcgata aaatagcctc tgaagatcaa
    25321 aatatacgac cgctcgcggc aagccgatgc cagatttgcg cttttttttc gtcgcgcccc
    25381 gcaaaattgc attccagtta acgcgctggc tgtatttgcc gcactgcgca ggtatactcc
    25441 tttcctggtt tttttaatca ctacgtcggg agttgttacc ccccatgcaa gaaattatgc
    25501 aatttgttgg ccgtcatccc atactgagta tcgcctggat cgcgttactg gtggcggttc
    25561 ttgtgactac gtttaagagc ctgacctcga aagtgaaggt gattactcgt ggtgaagcta
    25621 cgcgtctgat caacaaagaa gacgctgtgg ttgtggattt acgtcagcgt gatgacttcc
    25681 gtaaaggcca tatcgcaggt tctatcaacc tgttgccgag cgaaatcaaa gccaacaatg
    25741 ttggtgagct tgagaagcac aaagacaaac cggttatcgt ggtagatggt tctggcatgc
    25801 agtgccagga gcctgcaaac gcgctgacga aagctggttt tgcgcaagta ttcgtgctga
    25861 aagagggcgt tgctggctgg gctggcgaaa acttacctct ggtgcgcggc aaataattta
    25921 cctgtcgtct ttcatgatgc aggccgttag ctgcaattag gcccccagcg acttattgtt
    25981 ataagcttct ggggacactc cattttgcag ccgccctgca acaagaaatt tcagggtaaa
    26041 aaaacaggag ctaagttatg gccaatgttg aaatctatac caaagaaacc tgcccgtatt
    26101 gccatcgtgc gaaagcactg ctgagcagca agggcgtgag ttttcaggag ttgccgatcg
    26161 atggcaacgc cgctaagcgt gaagagatga tcaaacgcag tggtcgcacc acggttccac
    26221 agatttttat tgacgcacag cacattggtg gctgtgatga cttgtatgca ttggatgcac
    26281 gtggtggact ggatcccctg ctgaaataac gtgtgaacgt tggcattaca ttgcgcagta
    26341 tttaaggaca acacttaagg gttttctaca catgtcagaa caaaacaaca ccgaaatgac
    26401 tttccagatc caacgtattt ataccaagga tatttctttc gaagcgccga acgcgccgca
    26461 cgttttccag aaagattggc agccagaagt taaacttgat ctggatacgg catcttctca
    26521 actggcagat gacgtttacg aagtggtact gcgtgttacc gtaacggcct ccctgggcga
    26581 agaaaccgcg ttcctgtgtg aagttcagca gggcggtatt ttctccattg cgggtatcga
    26641 gggcacccag atggcacatt gcctgggtgc atactgcccg aacattctgt tcccgtatgc
    26701 tcgtgagtgc atcaccagca tggtatcccg cggtacattc ccgcaactga accttgcgcc
    26761 ggttaacttc gatgcgctgt tcatgaacta tttgcagcag caggctggcg aaggtactga
    26821 agaacatcag gatgcctgat gaaccaacgt aatgcttcaa tgactgtgat cggtgccggc
    26881 tcgtacggca ccgctcttgc catcaccctg gcaagaaatg gccacgaggt tgtcctctgg
    26941 ggccatgacc ctgaacatat cgcaacgctt gaacgcgacc gctgtaacgc cgcgtttctc
    27001 cccgatgtgc cttttcccga tacgctccat cttgaaagcg atctcgccac tgcgctggca
    27061 gccagccgta atattctcgt tgtcgtaccc agccatgtct ttggtgaagt gctgcgccag
    27121 attaaaccgc tgatgcgtcc tgatgcgcgt ctggtgtggg cgaccaaagg gctggaagca
    27181 gaaaccggac gtctgttaca ggacgtggcg cgtgaggcct taggcgatca aattccgttg
    27241 gcggttatct ctggcccaac gtttgcgaaa gaactggcgg caggtttacc gacggccatt
    27301 tcgctggcct cgacggacca gacctttgcc gatgacctcc agcaattgct gcactgtggc
    27361 aaaagtttcc gcgtttacag caatccggat ttcattggcg tgcagcttgg cggcgcggtg
    27421 aaaaacgtca ttgccattgg cgcggggatg tccgacggta tcggttttgg tgcgaatgcg
    27481 cgtacggcgc tgatcacccg tggactggct gaaatgtcgc gtcttggcgc ggcgctgggt
    27541 gccgatcctg ccacctttat gggcatggcg gggctgggcg atctggtgct tacctgtacc
    27601 gacaaccagt cgcgtaaccg ccgttttggc atgatgctcg gtcagggcat ggatgtacaa
    27661 agcgcgcagg agaaaattgg tcaggtggtg gaaggctacc gcaatacgaa agaagtccgc
    27721 gaactggcgc atcgcttcgg cgttgaaatg ccaataaccg aggaaattta tcaagtatta
    27781 tattgcggaa aaaacgcgcg cgaggcagca ttgactttac taggtcgtgc acgcaaggac
    27841 gagcgcagca gccactaaac ccagggaacc tttgttacca ctatgacccg gcccgcgcag
    27901 aacgggtcgg tcattatctt atcgtgtgga gtaagcaatg tcgtgtgaag aactggaaat
    27961 tgtctggaac aatattaaag ctgaagccag aacgctggcg gactgtgagc caatgctggc
    28021 cagtttttac cacgcgacgc tactcaagca cgaaaacctt ggcagtgcac tgagctacat
    28081 gctggcgaac aagctgtcat cgcccattat gccggctatt gctatccgtg aagtggtgga
    28141 agaagcctac gccgccgacc cggaaatgat cgcctctgcg gcctgtgata ttcaggcggt
    28201 gcgtacccgt gacccggcgg tggataaata ctcaaccccg ttgctatacc tgaagggttt
    28261 tcatgctttg caggcgtatc gcatcggcca ctggttgtgg aatcagggcc gccgcgcact
    28321 ggcaatcttt ctgcaaaacc aggtttctgt gacgttccag gttgatattc acccggcagc
    28381 aaaaattggt cgcggtatca tgcttgacca cgccacgggc atcgttgtgg gtgaaacggc
    28441 ggtgattgaa aacgacgtgt cgattctgca atctgtgacg cttggcggta cgggtaaatc
    28501 tggtggcgac cgtcatccga aaattcgtga aggtgtgatg attggcgcgg gcgcgaaaat
    28561 tctcggcaat attgaagttg ggcgcggcgc gaagattggc gcaggttccg tggtgctgca
    28621 accggtgccg ccgcatacca ccgccgctgg cgttccggca cgcattgtcg gcaagccgga
    28681 tagcgataaa ccgtcaatgg atatggatca gcatttcaac ggtattaacc atacgtttga
    28741 atatggggat gggatctgac gtcctgtgat cgtgccggat gcgatgtaat catctatccg
    28801 gcctacagta actaatctct caacaccgct cccggatacc ccaactgccg ccaggcttca
    28861 tacaccacga ccgacaccgc attggacaga ttcatgctgc ggctgtccgg caccattgga
    28921 atacgaattt tttgttcagc gggcagggca tcaagaatgc tcgctggcag gccgcgtgtt
    28981 tccgggccga acatcagata atcgccatcc tgatagctta cggcgctgtg agcaggcgta
    29041 cctttcgtgg tgagggcaaa caggcgctgg ggattttctg cttcgaggaa cgcgcgatag
    29101 tcatgatgac gcgtaacggc ggtaaactcg tgatagtcca gccccgcgcg gcgcaggcgc
    29161 ttatcgtccc aggcaaatcc catcggttcg atgatatgca gacgaaagcc ggtattagcg
    29221 caaagacgga tgatgttgcc agtatttggc ggaatttctg gttcgtaaag tacgatgttt
    29281 agcatactgc ccccttagtg cgggggcagg atagcagata ttcatctaac ccttaagctg
    29341 cattcccttt cgccattgga gccagcgccg ccgccggcgc tttactcagc tcttgcacca
    29401 gcgaatcttg cgtaatttcg ctaatcgatt tcgcgccagt cagcgtcatc gccactttca
    29461 tctctttttc gatcagattt agcaggttag ctacacccgc ctggcccgct gttgccagcg
    29521 catacaggaa agcacgaccc agcagtacgg tgtcggcacc gagcgcaatc atacgcacga
    29581 catcaagccc gttacgtatt ccgctatccg ccagaatggc aatatcacct ttcaccgcat
    29641 ccgcaatagc gggcaatgca cgagcggaag agagtacacc gtccagctgg cggccaccgt
    29701 ggttagaaac cacaatccca tcagcaccaa aacgtactgc atcgcgcgca tcttccggat
    29761 cgaggatccc tttgatcacc atcgggccat cccagaaatc gcggatccac tcaaggtctt
    29821 tccatgagat ggacggatcg aagttattcc ccagccagcc gatgtaatct tccagtccgg
    29881 tcggtttgcc gaggtaagcc gagatattac ctaaatcatg cggacgaccg ttcaggccca
    29941 catcccacgc ccattgcgga tgcgtcaccg cctgcaagta gcggcgcatt gccgcatttg
    30001 ggccgctcat cccagaatgc gcatcacggt aacgtgcgcc cggtgtcggc atatccacgg
    30061 tgaaaaccag tgtcgaacaa cccgctgctt ttgctcgctc cagtgcgtta cgcataaagc
    30121 cgcgatcgcg cagcacataa agctggaacc acatcggacg tttgatagcc ggagccactt
    30181 cttcaatcgg gcaaacggaa accgtcgaga gagtaaacgg aatgccatgc gcatccgctg
    30241 ctttggcagc ctgaacttcg ccgcgtcgcg catacatgcc acacaaaccc accggagcca
    30301 gcgccaccgg catcgacaat ttctcattaa acagcgtcgt ttccaggctt aagtcagaca
    30361 tgtttttcag aatacgctgg cgcagcgcca cttctgacaa atcttccacg ttgcggcgca
    30421 gcgtgtattc agaatatgca cccccatcca tatagtggaa caggaacggc ggcagaatgc
    30481 gttgtgctgc ggcgcgataa tcgctggctg cggaaataat catgcgtttt tctccctcga
    30541 atgctcatta tggtcaccag gcaggcgggt aatgcgtgca tggcgagctt gatcttcatc
    30601 gaatcgtttc atggtggtgt gaacaaagct aaggtgcgcc atcattgctt tacgcgcccc
    30661 gtcagcatca ccggcaaaaa tggcgtcaat gactgcctga tgttgttcgg tcagctgtga
    30721 aaaaaccggg ggtaccagat acatccgctg gcggctatgc ttcacggagg attgcaggac
    30781 atcgaagaaa ccgcgcatgg tttgcagcag cacgatgtta tgcgaggctt cggcaatcgc
    30841 cagatgaaaa cgaacgtccg cttgcgaagc gaggtccggg tcttcgctga gcgttgcttc
    30901 aaaacaaagc tgaatctttt ctttgtcgcc aggtgtggcg cgcattgccg catgccacgc
    30961 ggtgctggct tcaatggcgt agcgggcttc cagaatatcg aaactgtaat ccggatcatc
    31021 ggccatcagc gttttcagcg gctggacgat gttttgctcc gaccatgtgt catgacgcca
    31081 gcgaataaac gtcccgccgc cacgtcgact gagcagcaca ccttcgctta ccagtttcgc
    31141 cagcgcctcg cgcagtgaat ttctcgacac gccgagttgc atcgccagtt gacgttcagc
    31201 gggcaacttc atgcccgctt ccaggttttt ttcatcaatc agcgcccgca cacgatcggc
    31261 aacctcgtct gacaggcgtc tgggtaaaac aatcattaag gaatcatcca cgttaagaca
    31321 taggcctgaa gcgtggtgat cacgcccacc atacaggtga agatcaggct gtgtttgaca
    31381 gtaaagcgga acaaatcaga ctctttgccc accaggccta ccgccgcaca ggcgatagcg
    31441 atagattgcg gggagatcat cttaccggtg acgccaccgg tggtattggc ggcaaccaac
    31501 aacaggtcag aaacgccaat ttgttgtgcg gcggtagcct gcaaagcggc aaacaaagca
    31561 ttagatgacg tatccgaccc ggtcaggaat accccaagcc agccgaggaa cggcgagaag
    31621 aaggtgaatg catgaccggt gtgcgccagt gccagcgcca gtgttgatga caaaccggaa
    31681 tagttcgaga taaaggcgaa tgccagcacc ataccgatgg agtagatggg cagagccagt
    31741 tctttcagcg tgctgccgaa ggtgctgata gcgtcagatg gtttcatctt cagccagaca
    31801 atcgagagca gggcagcaaa cagaatggcg gtaccggtgg cagagaacca gtcaaactta
    31861 aacacggcgg catacgctgt cgcttcgctg accactggcg gcatacgggc aaccagttta
    31921 tcgaggtacg gcaccggaat gttgatcacc cactcataca gcgcaccgcc tgaagcgaac
    31981 agggctttaa acggcgggat actccacagc gttacggtgg cggtcaggaa caggaacggt
    32041 gtccaggcgc gcagcacctg acctgccgtg taacctgtgt gagccagcgt catatcaacc
    32101 tgtgacgccc ctaagtcacc gaagcggaat acgcgcactg gttgccagcg tttgaggaac
    32161 agcgtcaggc agagcagtga taccagcgaa gagataatgt ctggcagttc cggcccaatg
    32221 aagttagagc tgaggtattg agcaatggca aacgaaccac ctgcaaccac gaccgcaggc
    32281 cacgtctctt tgataccgcg ccaaccgtcc ataatcgcca tgatccagaa cagcacgata
    32341 atggtcataa acggcagctg acgccccacc atctgaccaa tctcaaagct gtcgatgcct
    32401 gttacctgcc cggcgaccag aatcggaata cccatcgcgc caaatgccac tggtgcggtg
    32461 ttaacaatca ggcacagtcc ggcggcgtag agtggtttaa agcccagacc gaccagcaat
    32521 gcggcggtaa ttgctaccgg tgcgccaaag cctgcggctc cttcaaggaa agcgccgaaa
    32581 cagaaaccga caatcagcat ttgcaggcgc tggtcagggg ttatcgaaag aatagacgag
    32641 cggatgatgt caaactgccc ggttttcacc gagatcttat agacgaacac cgctgcaata
    32701 atgatccacg cgatgggcca caacccgtag aagaagccat aaaccaccga ggccagcgcg
    32761 ttagcgaccg gcattttata gaacagcaaa gccacggcaa gggcgattgc caccgtccat
    32821 gaggcggcga cgtatccttt cagtttgagc ttaatcagcg caaagaagaa aaacaggatg
    32881 ggaagcgatg ctatcaggct ggaaagccag atattcccgg cgggatcgta gttttgttgc
    32941 cagagagtca ttgcaggtct cctgcagtcc acacacgccg tgtaatgagt ctgttgctca
    33001 tctccttgtc acacgttgtg taaaagtggc actgccaatt gtgttgtgta gggataatga
    33061 caatggatag ttaactaaat gttatgcatt agcaacattg atgtgagttt taattgggaa
    33121 attgtgaacc agaggtcaga taagcgaaga attggtaggg ccaattcttg gtggagggag
    33181 atgtctggta aagaaaaggg caatcctcat cagagaattg cccttagtca cactaagtta
    33241 atggatcatt accactgaat accggcaccg agtgcggcgg agtattcacc ctggctattg
    33301 gtactacctt gtaatttgta gacccaacga ccattggcgc tcaccatcga cacacctaaa
    33361 gcaacagccg attcaccgtt gtaagtacca ccaccaatag aggccatgct ggctcccggc
    33421 gtgtaagcct gcggcagacc ggtcattgcc attgcggaag cgataccacc gctcaacttg
    33481 ctttcggttt tggacagttt gttatccatc tcaaccatgc gctgatcggt gtattgcttc
    33541 gtttcctgca cactttgctt cagctgcgca tagttcaccg cgtcgttgtt gttcacgcca
    33601 gcagaaacgt tgctgatgcg agtcgtaccg ccatttccgc caccgagagt gatgttgctg
    33661 tagtcgatag agccatcagc tttggtgtcg tagcgaacgc cgcctgcttc agaagacttc
    33721 agttgtgaaa cgttaaccgc atcggtggca ttaacaccgg cagcaacgtt ggtgatacgg
    33781 cgctggttgg tagaagaacc cacagagatg gtgttttctt cgttggccac ggaaccggta
    33841 cccagcgcga cgctgttgtc agcggcagca atggaaccag aaccaattgc aacactgtct
    33901 ttaccctgcg cgttggcatc tacgccatcg gtgttggtct tgaagtattt ggtgctaccg
    33961 gtggttacga tatcgccaat gccgttttcg atattggtca cgcgagtatc cagattcgtc
    34021 acctgattgt tcaggttagt aatgctctgg gtattctggg aaacctgcgc gtccgttact
    34081 ttcaactgac ccacgttaac cgcatcggta tccgctgaac cagccgcgac gttggtgatc
    34141 tgacgttgac cgtcttcact gccgacagag aactcaccca cagagttctg tggtgcgtcc
    34201 atgttgtagg cgctgtagtt ggtctgcgca ccacgggtag tctgagaacc attccccatt
    34261 gcaatgctgt ttgcatggtt tgcacttgcg ttgctaccga tagcaatacc attgatcgca
    34321 tcagccagaa ctaaagtgtt caggccgata ccaatacccg cattaccatt aacgatggtt
    34381 tttgcgccca ttgccagcga gtcttcaccg actgccagtg agtcttctgc cgttgagttg
    34441 gcgtgatagt acttggttgg cgtagtggcg accgcgccaa tagcgttttg caactggcga
    34501 accgtaacgg cgtcatgggc ttcggaacca tcggctacgt tgatgatttg acgatattta
    34561 ccatcgtcac cgatcgacaa tgcgccaagc agttcaccat cagttgtgtc ataaccaatc
    34621 acaacacctt cttccgacac gctggtgtca cgagaacctt taactattaa acggctggaa
    34681 acggaactgc tacccagggc gatacccgtc tcaacttcgc tgcggctgtc ctgaccaatg
    34741 gctacgctgc tggctttgga agcaacagag ttataaccca ctgctgtagc gccaacaccc
    34801 tgtgcgctgg catcggtgaa ggttaaaccg gtgtcattgg tacgcacata gttaatacct
    34861 gcaccatttt cttcgatgta ggtttcactg gtgttaccag caagctgatt aatgacttcg
    34921 ctgttctgct ggatcagcat attggtgtta aacagctgag aaccgttgat agcatcggtg
    34981 ctgccttccc cgatattacc atcagcgaca ttagtgatga tgctggcttt gccatcatga
    35041 ctggcgttgt aagccccttc gccgttattg gctgtcgcat cccacagcaa cgcgttctca
    35101 tcaagcgcat ccagagcttc accgacgttg ttgtaatcgg tatttgcaat ggtgtaagtc
    35161 ggcgcagtga tagtgccgtc agcgttgact tctgcgttac cgcccagcgc atcagcgatg
    35221 tacttattgg tggtatagag ttgtgagccg ttaatcgcgt cgctgctggt ggcagagatt
    35281 gcaccatttg cgacgttagt aatcttactg gcggtgttat ctaccccgcg accggcgctg
    35341 aatgcaccag caccgtcatt ggctgtcgca tcccagagca gggcatcacc gagagaatca
    35401 ctaaatgaag tgttaattgc ggccagagca tccccgacgt tagtgtagtc agtgccgcca
    35461 atactatagg ttggcccggt taatacacct gtatcgctaa tatcagcacc accacccaga
    35521 taggacgtga atgaatcagc caaaccatat agttgtgagc catttatggc gtcagtgctg
    35581 tcagaggaga ctatgccagc ttgtacgttg ctgattttgt tggtggcgtt agtaccgtgt
    35641 gcagcggtga atgcgcctgc ggcgtcgtcc catttcaggg cgtcttcacc gagattattc
    35701 accgtatcag tcaggttagt gatattggtg gtgttagtgg cgatattggt ggtgtt
//
LOCUS       DAMZFT010000038        35648 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.038, whole genome shotgun sequence.
ACCESSION   DAMZFT010000038 DAMZFT010000000
VERSION     DAMZFT010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 35648)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35648)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35648
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.038"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..422
                     /gene="btuE"
                     /locus_tag="PN977_001959"
     CDS             <1..422
                     /gene="btuE"
                     /locus_tag="PN977_001959"
                     /EC_number="1.11.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional thioredoxin/glutathione peroxidase"
                     /protein_id="HDI6041990.1"
                     /translation="QLENIQKAWADRGFVVLGFPCNQFLEQEPGSDEEIKTYCTTTWG
                     VTFPMFSKIEVNGEGRHPLYQKLIAAAPTAVAPEESGFYARMVSKGRAPLYPDDILWN
                     FEKFLVGRDGLVIQRFSPDMTPEDPIVMESIKLALAK"
     gene            422..1171
                     /gene="btuD"
                     /locus_tag="PN977_001960"
     CDS             422..1171
                     /gene="btuD"
                     /locus_tag="PN977_001960"
                     /EC_number="7.6.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter ATP-binding protein
                     BtuD"
                     /protein_id="HDI6041991.1"
                     /translation="MSIVMQLQDVAESTRLGPLSGEVRAGEILHLVGPNGAGKSTLLA
                     RMAGMTSGKGSIQFAGQPLEAWSATKLALHRAYLSQQQTPPFAMPVWHYLTLHQHDKT
                     RTELLNDVAGALALDDKLGRSTNQLSGGEWQRVRLAAVVLQITPQANPAGQLLLLDEP
                     MNSLDVAQQSALDKILSALCQQGLAIVMSSHDLNHTLRHAHRAWLLKGGKMLASGRRE
                     EVLTPPNLAQAYGMNFRRLDIEGHRMLISTI"
     gene            1249..1713
                     /locus_tag="PN977_001961"
     CDS             1249..1713
                     /locus_tag="PN977_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HDI6041992.1"
                     /translation="MRFCLILITALFLAGCSHHKAPPPNARLSDSITVIAGLNDQLQS
                     WHGTPYRYGGMTRRGVDCSGFVVVTMRDRFDLQLPRETKEQASIGTQIDKDELLPGDL
                     VFFKTGSGQNGLHVGIYDTNNQFIHASTSKGVMRSSLDNVYWQKNFWQARRI"
     gene            1960..2673
                     /gene="ydiV"
                     /locus_tag="PN977_001962"
     CDS             1960..2673
                     /gene="ydiV"
                     /locus_tag="PN977_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-FlhDC factor"
                     /protein_id="HDI6041993.1"
                     /translation="MKIFLENLYHSDCYFLPIRDNQQDLVGVELITHFSSEDGTVRIP
                     TSRVIAQLTEEQHWQLFSEQLELLKSCQHFFIQHKLFAWLNLTPQVATLLLDRDNYAG
                     ELLKYPFIELLINENYPHLDEGKDNRDLFSLSQMYPLVLGNLGAGNSTMKAVFDGLFT
                     RVMLDKSFIQQQITHRSFEPFIRAIQAQISPCCNCIIAGGIDTPEIMAQITPFDFHAL
                     QGCLWPAVPVNQITTLVQR"
     gene            2736..4172
                     /locus_tag="PN977_001963"
     CDS             2736..4172
                     /locus_tag="PN977_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiU family protein"
                     /protein_id="HDI6041994.1"
                     /translation="MTLSFITRWCDELPETYTALSPTPLNKARLIWHNAELANTLSIP
                     SSLFKNGAGVWGGETLLPGMSPLAQVYSGHQFGVWAGQLGDGRGILLGEQQLADGTTM
                     DWHLKGAGLTPYSRMGDGRAVLRSTIRESLASEAMHYLGIPTTRALSIVSSDSPVYRE
                     TVEPGAMLMRVAPSHLRFGHFEHFYYRREPEKVRQLADFAIRHYWSHLADDEDKYRLW
                     FSDVVARTASLIAQWQTVGFAHGVMNTDNMSLLGLTLDYGPFGFLDDYEPGFICNHSD
                     HQGRYSFDNQPAVALWNLQRLAQTLSPFVAVDALNEALDSYQQVLLTHYGQRMRQKLG
                     FMTEQKEDNALLNELFSLMARERSDYTRTFRMLSLTEQHSAASPLRDEFIDRAAFDDW
                     FARYRRRLQQDEVSDIERQQLMQSVNPALVLRNWLAQRAIEAAEKGDMTELHRLHEAL
                     RNPFSDRDDDYVSRPPDWGKRLEVSCSS"
     gene            complement(4176..4367)
                     /gene="hemP"
                     /locus_tag="PN977_001964"
     CDS             complement(4176..4367)
                     /gene="hemP"
                     /locus_tag="PN977_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemin uptake protein HemP"
                     /protein_id="HDI6041995.1"
                     /translation="MRYTDSRKFTPETDANHKTASPQPIRRITSQTLLGPDGKLIIDH
                     DGQEYLLRKTQAGKLLLTK"
     gene            complement(4499..5545)
                     /gene="aroH"
                     /locus_tag="PN977_001965"
     CDS             complement(4499..5545)
                     /gene="aroH"
                     /locus_tag="PN977_001965"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroH"
                     /protein_id="HDI6041996.1"
                     /translation="MNRTDELRTARIESLVTPAELALRYPVTPGVATHVTDSRRRIEK
                     ILNGEDKRLLVIIGPCSIHDLTAAMEYATRLQSLRNQYQSRLEIVMRTYFEKPRTVVG
                     WKGLISDPDLNGSYRVNHGLELARKLLLQVNELGVPTATEFLDMVTGQFIADLISWGA
                     IGARTTESQIHREMASALSCPVGFKNGTDGNTRIAVDAIRAARASHMFLSPDKNGQMT
                     IYQTSGNPYGHIIMRGGKKPNYHADDIAAACDTLHEFDLPEHLVVDFSHGNCQKQHRR
                     QLEVCEDICQQIRNGSTAIAGIMAESFLREGTQKIVGGQPLTYGQSITDPCLGWEDTE
                     RLVEKLASAVDTRF"
     gene            complement(5702..6535)
                     /gene="ppsR"
                     /locus_tag="PN977_001966"
     CDS             complement(5702..6535)
                     /gene="ppsR"
                     /locus_tag="PN977_001966"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="posphoenolpyruvate synthetase regulatory
                     kinase/phosphorylase PpsR"
                     /protein_id="HDI6041997.1"
                     /translation="MDNAVDRHVFYISDGTAITAEVLGHAVMSQFPVTISSITLPFVE
                     NESRARAVKDQIDAIYHQTGVRPLVFYSIVLPEIRAIILQSEGFCQDIVQALVAPLQQ
                     EMKLDPTPIAHRTHGLNPNNLNKYDARIAAIDYTLAHDDGISLRNLDQAQVILLGVSR
                     CGKTPTSLYLAMQFGIRAANYPFIADDMDNLVLPASLKPLQHKLFGLTIDPERLAAIR
                     EERRENSRYASLRQCRMEVAEVEALYRKNQIPWINSTNYSVEEIATKILDIMGLSRRM
                     Y"
     gene            6867..9245
                     /gene="ppsA"
                     /locus_tag="PN977_001967"
     CDS             6867..9245
                     /gene="ppsA"
                     /locus_tag="PN977_001967"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HDI6041998.1"
                     /translation="MSNNGSSPLVLWYNQLGMNDVDRVGGKNASLGEMITNLSGMGVS
                     VPNGFATTADAFNQFLDQSGVNQRIYELLDKTDIDDVTQLAKAGAQIRQWIIDTPFQP
                     ELENAIREAYAQLSADDENASFAVRSSATAEDMPDASFAGQQETFLNVQGFDAVLVAV
                     KHVFASLFNDRAISYRVHQGYDHRGVALSAGVQRMVRSDLASSGVMFSIDTESGFDQV
                     VFITSAWGLGEMVVQGAVNPDEFYVHKPTLAANRPAIVRRTMGSKKIRMVYAPTQEHG
                     KQVKIEDVPQEQRDIFSLTNEEVQELAKQAVQIEKHYGRPMDIEWAKDGHTGKLFIVQ
                     ARPETVRSRGQVMERYTLHSQGKIIAEGRAIGHRIGAGPVKVIHDISEMNRIEPGDVL
                     VTDMTDPDWEPIMKKASAIVTNRGGRTCHAAIIARELGIPAVVGCGDATERMKDGENV
                     TVSCAEGDTGYVYAELLEFSVKSSSVETMPDLPLKVMMNVGNPDRAFDFACLPNEGVG
                     LARLEFIINRMIGVHPRALLEFDDQEPQLQNEIREMMKGFDSPREFYVGRLTEGIATL
                     GAAFYPKRVIVRLSDFKSNEYANLVGGERYEPDEENPMLGFRGAGRYVSDSFRDCFAL
                     ECEAVKRVRNDMGLTNVEIMIPFVRTVDQAKAVVEELARQGLKRGENGLKIIMMCEIP
                     SNALLAEQFLEYFDGFSIGSNDMTQLALGLDRDSGVVSELFDERNDAVKALLSMAIRA
                     AKKQGKYVGICGQGPSDHEDFAAWLMEEGIDSLSLNPDTVVQTWLSLAELNK"
     gene            9734..11134
                     /locus_tag="PN977_001968"
     CDS             9734..11134
                     /locus_tag="PN977_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001278076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion permease"
                     /protein_id="HDI6041999.1"
                     /translation="MSCYLKVLICAIVALAIWFFPVPDGLTPLTWHILAIFITTVVAF
                     ILQPLPVGAIALIAISFIMLTGMMKTSEALKGFSSTTVWLIVAAFLYAKGFIKTGLGR
                     RIAYLLIRGFGGSSLRLGYTLALSDMIIAPATPSNTARAGGILFPIVRSVSNSFGSEP
                     DQGPRKIGAYLMQTVFHSNCLSSSMFMTASAPNALIVSLAASTLFVDISWGMWTLSAL
                     VPGIIAFITMPLVIYKLYPPEIKKTPEAKALAQEELIKMGPVTRDERVTIGIFLLSLL
                     AWSTSKWTGLDATAVALSGVCLMLMTRIITWQDVQSEKGAWDILVWLGVMICMADKLN
                     QLGLFKWFAVTTSALFTGIPWEITLTVLLIVYCYSHYFFAGSTPHVVAMYAAFGSVSV
                     AAGAPPMMAALSLAFVTNLMSGISHYGNGPAVIYYGAGYVSQREWWRLGFIVMLLNIA
                     IWFGLGAVWWKILGLW"
     gene            11170..12129
                     /locus_tag="PN977_001969"
     CDS             11170..12129
                     /locus_tag="PN977_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase"
                     /protein_id="HDI6042000.1"
                     /translation="MNLKDIQVAIVGKLSDRLMAKVESTFTAYRLWELADEGAFYTQI
                     APDIQALVTSGNPVMGASRALIEKFPNLKIIASNGVGYDPIDIVAAREHGVIVTNTPG
                     VLNDCVADIGIALLLNVARRINIADCYVRDGRWPNEGRFPMATKVSGKRCGIVGLGNI
                     GNAVARRAAAFEMEIHYFDPKPHNKPDWTAHDSLVSLAQAVDFLVLTLPGGASTRGVI
                     DKTVLQALGKTGHLISISRGSVVNETDLIDALQNNIIAGAALDVYANEPHVPEQLMSL
                     DNVGLTPHIASGTSETFNAMADLVFDNLQAFFSGRPVITPVTQ"
     gene            12140..12619
                     /locus_tag="PN977_001970"
     CDS             12140..12619
                     /locus_tag="PN977_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000639093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HDI6042001.1"
                     /translation="MIYGDIAYLSEQQKRTFHPVITRVIEYLSQHDLNSLPVGEKVFI
                     QDDSIFYTVVEPMTKPLHECRPEVHRKYLDVHFLIAGEERICVDFDDGENSVVESHLG
                     DRDVIFYADERINDRIITLTPGQFVVVFPEEVHRACYKIDNVEKIKKTVVKIDMSLL"
     gene            12632..13375
                     /locus_tag="PN977_001971"
     CDS             12632..13375
                     /locus_tag="PN977_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000160965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDI6042002.1"
                     /translation="MTKIALVTGGSKGIGYAAAKALYDIGYTVIVAARNEETLQQCAQ
                     GLAKERFIPLVADVTDESAVVALFAEIKALFGRLDLLFNNAGNNSPAKSIEEVEFTEW
                     TRVFNANIHSTFLCSKEAIKIMKAQQPMGGRIINNASISALTPRLYTAPYTASKHAVT
                     GLTKAISLDCRKFNIACGQINIGNAETTLSNRMRKGVYQADGAIKAEDMMNVADVAAT
                     IQMMAQLPVTTNVLEITIMANNMPYVGRG"
     gene            complement(13426..15066)
                     /gene="fadK"
                     /locus_tag="PN977_001972"
     CDS             complement(13426..15066)
                     /gene="fadK"
                     /locus_tag="PN977_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001362104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="medium-chain fatty-acid--CoA ligase"
                     /protein_id="HDI6042003.1"
                     /translation="MKVTLTFNEQRRAAYRQQGLWGDASLADYWQQTARAMPDKIAVV
                     DNHGASYTYSALDHAASCLANWMLTKGIESGDRIAFQLPGWCEFTVIYLACLKIGAVS
                     VPLLPSWREAELVWVLNKCQAKMFFAPTLFKQTRPVDLILPLQNQLPQLQQIVGVDKL
                     APATSSLSLSQILADNTPLTTAITTHGDELAAVLFTSGTEGLPKGVMLTHNNILASER
                     AYCARLNLTWQDVFMMPAPLGHATGFLHGVTAPFLIGARSVLLDIFTPAACLALLEQQ
                     RCTCMLGATPFVYDLLNLLEKQPADLSALRFFLCGGTTIPKKVARECQQRGIKLLSVY
                     GSTESSPHAVVNLDDPLSRFMHTDGYAAAGVEIKVVDGARKTLPPGCEGEEASRGPNV
                     FMGYFDEPELTAHALDEEGWYYSGDLCRMDEAGYIKITGRKKDIIVRGGENISSREVE
                     DILLQHPKIHDACVVAMPDERLGERSCAYVVLKAPHHSLSLEDVVAFFSRKRVAKYKY
                     PEHIVVIEKLPRTASGKIQKFLLRKDILQRLEQTCVEA"
     gene            complement(15123..15416)
                     /locus_tag="PN977_001973"
     CDS             complement(15123..15416)
                     /locus_tag="PN977_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HDI6042004.1"
                     /translation="MSQNATVNVDIKLGVNKFHVDEGHPHIILAANPDINEFRKLMKA
                     CPAGLYKQDDAGNIHFDSAGCLECGTCRVLCGNTILEQWQYPAGTFGIDFRYG"
     gene            complement(15413..16702)
                     /locus_tag="PN977_001974"
     CDS             complement(15413..16702)
                     /locus_tag="PN977_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HDI6042005.1"
                     /translation="MSDDKFDAIVVGAGVAGSVAALVMAQAGLDVLVIERGDSAGCKN
                     MTGGRLYAHTLEAIIPGFAASAPVERKVTREKISFLTDESAVTLDFHREQPDVPQHAS
                     YTVLRNRLDPWLMEQAEQAGAQFIPGVRVDALVREGNKVTGVQAGDDILEANVVILAD
                     GVNSMLGRSLGMVPASDPHHYAVGVKEVIGLTPEQIKDRFNITGEEGAAWLFAGSPSD
                     GLMGGGFLYTNKDSISLGLVCGLGDIAHAQKSVPQMLEDFKQHPAIHPLISGGKLLEY
                     SAHMVPEGGLAMVPQLVNDGVMIVGDAAGFCLNLGFTVRGMDLAIASAQAAATTVIAA
                     KERADFSASSLAQYKRELEQSCVMRDMQHFRKIPALMENPRLFSQYPRMVADIMNEMF
                     TIDGKPNQPVRKMIMGHAKKIGLINLLKDGIKGATAL"
     gene            complement(16757..17695)
                     /locus_tag="PN977_001975"
     CDS             complement(16757..17695)
                     /locus_tag="PN977_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit alpha"
                     /protein_id="HDI6042006.1"
                     /translation="MSQLNSVWVFSDNPERYAELFGGAQQWGQQVYAIVQNTEQAQAV
                     MPYGPKCIYVLEQNDALQRTENYAESIAALLKDKHPAMLLLAATKRGKALAARLSVQL
                     NAALVNDATAVDIVDGHICAEHRMYGGLAFAQEKINSPLAIITLAPGVQEPCTRDTSH
                     QCPTETVPYVAPRHEILCRERRAKAASSVDLSKAKRVVGVGRGLAAQDDLKMVHELAA
                     VLNAEVGCSRPIAEGENWMERERYIGVSGVLLKSDLYLTLGISGQIQHMVGGNGAKVI
                     VAINKDKNAPIFNYADYGLVGDIYKVVPALISQLSR"
     gene            complement(17715..18479)
                     /locus_tag="PN977_001976"
     CDS             complement(17715..18479)
                     /locus_tag="PN977_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein"
                     /protein_id="HDI6042007.1"
                     /translation="MKIITCFKLVPEEQDIVVTPEYTLNFDNADAKISQFDLNAIEAA
                     SQLASDDDEIAALTIGGSLLQNSKVRKDVLSRGPHSLYLVQDAQLEHALPLDTAKALA
                     AAVEKIGFDLLIFGEGSGDLYAQQVGLLVGEILQLPVINAVSAIQRQGNTLVIERTLE
                     DDVEVIELSVPAVLCVTSDINMPRIPSMKAILGAGKKPVNQWQASDIGWSQSAPLAEL
                     TGIRVPPQTERKHIILDNDSPEAIAELAEHLKKALN"
     gene            18795..19706
                     /locus_tag="PN977_001977"
     CDS             18795..19706
                     /locus_tag="PN977_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HDI6042008.1"
                     /translation="MYQRCFDNASETLFVAGKTPRLSRFAFSDDPKWESGHHVHDNET
                     ELIYVKKGVARFTIDSSLYVAHADDIVVIERGRLHAVASDVNDPATTCTCALYGFQFQ
                     GAEENQLLQPHSCPVIAAGQGKEVIKTLFNELSVILPQSKNSQTSSLWDAFAYTLAIL
                     YYENFKNAYRSEQGYIKKDVLIKDILFYLNNNYREKITLEQLSKKFRASVSYICHEFT
                     KEYRISPINYVIQRRMTEAKWSLTNTELSQAEISWRVGYENVDHFAKLFLRHVGCSPS
                     DYRRQFKNCFAEQEILSEFPQPVSLAG"
     gene            complement(19749..20900)
                     /locus_tag="PN977_001978"
     CDS             complement(19749..20900)
                     /locus_tag="PN977_001978"
                     /EC_number="1.3.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HDI6042009.1"
                     /translation="MDFSLTEEQELLLASIRELITTNFPEEYFRTCDQNGTYPREFMR
                     ALADNGISMLGVPEEFGGIPADYVTQMLALMEVSKCGAPAFLITNGQCIHSMRRFGSA
                     EQLRKTAESTLETGDPAYALALTEPGAGSDNNSATTTYTRKNGKVYINGQKTFITGAK
                     EYPYMLVLARDPQPKDPKKAFTLWWVDSSKPGIKINPLHKIGWHMLSTCEVYLDNVEV
                     EESDMVGEEGMGFLNVMYNFEMERLINAARSTGFAECAFEDAARYANQRIAFGKPIGH
                     NQMIQEKLALMAIKIDNMRNMVLKVAWQADQHQSLRTSAALAKLYCARTAMEVIDDAI
                     QIMGGLGYTDEARVSRFWRDVRCERIGGGTDEIMIYVAGRQILKDYQNK"
     gene            complement(20914..22509)
                     /locus_tag="PN977_001979"
     CDS             complement(20914..22509)
                     /locus_tag="PN977_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl CoA:acetate/3-ketoacid CoA transferase"
                     /protein_id="HDI6042010.1"
                     /translation="MKPVKPPRINGRVPVLSAQEAVNYIPDEATLCVLGAGGGILEAT
                     TLITALADKYKQTQTPRNLSIISPTGLGDRADRGISPLAQEGLVKWALCGHWGQSPRI
                     SDLAEQNKIIAYNYPQGVLTQTLRAAAAHQPGIISDIGIGTFVDPRQQGGKLNEVTKE
                     DLIKLVEFDNKEYLYYKSIAPDIAFIRATTCDSEGYATFEDEVMYLDALVIAQAVHNN
                     GGIVMMQVQKMVKKATLHPKSVRIPGYLVDIVVVDPDQTQLYGGAPVNRFISGDFTLD
                     DSTKLSLPLNQRKLVARRALFEMRKGAVGNVGVGIADGIGLVAREEGCADDFILTVET
                     GPIGGITSQGIAFGANVNTRAILDMTSQFDFYHGGGLDVCYLSFAEVDQHGNVGVHKF
                     NGKIMGTGGFIDISATSKKIVFCGTLTAGSLKTEITDGKLNIVQEGRVKKFIRELPEI
                     TFSGKIALERGLDVRYITERAVFTLKEDGLHLIEIAPGVDLQKDIFDKMDFTPVISPE
                     LKLMDERLFIDAAMGFVLPEAAH"
     gene            complement(22655..23413)
                     /gene="aroD"
                     /locus_tag="PN977_001980"
     CDS             complement(22655..23413)
                     /gene="aroD"
                     /locus_tag="PN977_001980"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HDI6042011.1"
                     /translation="MKTVTVKDLVIGTGAPKIIVSLMAKDIASVKSEALAYREADFDI
                     LEWRVDHYADLSNVESVMAAAKILRETMPEKPLLFTFRSAKEGGEQAISTEAYIALNR
                     AAIDSGLVDMIDLELFTGDDQVKETVAYAHAHDVKVVMSNHDFHKTPEAEEIIARLRK
                     MQSFDADIPKIALMPQSTSDVLTLLAATLEMQEQYADRPIITMSMAKTGVISRLAGEV
                     FGSAATFGAVKKASAPGQISVNDLRTVLTILHQA"
     gene            complement(23444..24310)
                     /gene="ydiB"
                     /locus_tag="PN977_001981"
     CDS             complement(23444..24310)
                     /gene="ydiB"
                     /locus_tag="PN977_001981"
                     /EC_number="1.1.1.282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinate/shikimate dehydrogenase"
                     /protein_id="HDI6042012.1"
                     /translation="MNVTAKYELIGLMAYPIRHSLSPEMQNKALEKAGLPFTYMAFEV
                     DNDSFPAAIEGLKALKMRGTGVSMPNKQLACEYVDELTPAAKLVGAINTIVNDDGYLR
                     GYNTDGTGHIRAIKESGFDIKGKTMVLLGAGGASTAIGAQGAIEGLKEIKLFNRRDEF
                     FDKALAFAQRVNENTDCVVTVTDLADQQAFAEALASADILTNGTKVGMKPLENESLVH
                     DISLLHPGLLVTECVYNPHMTKLLQQAQQAGCKTIDGYGMLLWQGAEQFTLWTGKDFP
                     LEYVKQVMGFGA"
     gene            complement(24322..25587)
                     /locus_tag="PN977_001982"
     CDS             complement(24322..25587)
                     /locus_tag="PN977_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDI6042013.1"
                     /translation="MSQNKAFSTPFILAVLCIYFSYFLHGISVITLAQNMTSLAEKFS
                     TDNAGIAYLISGIGLGRLISILFFGVISDKFGRRAVILMAVIMYLLFFFGIPACPNLT
                     LAYCLAVCVGIANSALDTGGYPALMECFPKASGSAVILVKAMVSFGQMFYPMLVSYML
                     LNNIWYGYGLIIPGILFVLITLMLLKSKFPSQLVDASVANELPQMNSKPLVWLEGVSS
                     VLFGVAAFSTFYVIVVWMPKYAMAFAGMSEAEALKTISYYSMGSLVCVFIFAALLKKM
                     VRPIWANAFNSALATITAAIIYLYPSPLVCNAGAFVIGFSAAGGILQLGVSVMSEFFP
                     KSKAKVTSIYMMMGGLANFVIPLITGYLSNIGLQYIIVLDFTFALLALITAIIVFIRY
                     YRVFIIPENDVRFGEHKFSTRLNTIKHRG"
     gene            complement(25814..27028)
                     /locus_tag="PN977_001983"
     CDS             complement(25814..27028)
                     /locus_tag="PN977_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDI6042014.1"
                     /translation="MKNPYYPTALGLYFNYLVHGMGVILMSLNMASLETLWQTNAAGV
                     SIVISSLGIGRLSVLLFAGLLSDRFGRRPFIMLGMCCYMAFFFGILHTNNIIIAYVFG
                     FLAGMANSFLDAGTYPSLMEAFPRSPGTANILIKAFVSSGQFLLPLIISLLVWAELWF
                     GWSFMIAAGIMFINALFLYRCTFPPHPGRRLPVIKKTTSSTEHRCSIIDLASYTLYGY
                     ISMATFYLVSQWLAQYGQFVAGMSYTMSIKLLSIYTVGSLLCVFITAPLIRNTVRPTT
                     LLMLYTFISFIALLTVCLHPTFYVVIIFAFVIGFTSAGGVVQIGLTLMAERFPYAKGK
                     ATGIYYSAGSIATFTIPLITAHLSQRSIADIMWFDTAIAAIGFILALFIGLRSRKETR
                     HHSLKENVAPGG"
     gene            complement(27128..27484)
                     /locus_tag="PN977_001984"
     CDS             complement(27128..27484)
                     /locus_tag="PN977_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707588.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiL family protein"
                     /protein_id="HDI6042015.1"
                     /translation="MNAYELQALRHIFAMTIDECATWIAQTGDSESWRQWENGKCAIP
                     DCVVEQLLAMRQQRKKHLHAIIEKINNRIGNNTMRFFPDLTAFQQVYPDGNFIDWKIY
                     QSVAAELYAHDLERLC"
     gene            complement(27620..27727)
                     /gene="rprA"
                     /locus_tag="PN977_001985"
     ncRNA           complement(27620..27727)
                     /ncRNA_class="antisense_RNA"
                     /gene="rprA"
                     /locus_tag="PN977_001985"
                     /product="antisense sRNA RprA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00034"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00034"
     gene            complement(27913..29025)
                     /gene="ydiK"
                     /locus_tag="PN977_001986"
     CDS             complement(27913..29025)
                     /gene="ydiK"
                     /locus_tag="PN977_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter YdiK"
                     /protein_id="HDI6042016.1"
                     /translation="MVNVRQPRDVAQILLSVLFLAIMIVACLWIVQPFILGFAWAGTV
                     VIATWPVLLRLQKFMFGRRSLAVLVMTLLLVMVFIIPIALLVNSIVDGSGPLIKAISS
                     GDMTLPDLAWLNTIPVIGAKLYAGWHNLLDMGGTAIMAKVRPYIGTTTTWFVGQAAHI
                     GRFMVHCALMLLFSALLYWRGEQVAQGIRHFATRLAGVRGDAAVLLAAQAIRAVALGV
                     VVTALVQAVLGGIGLAVSGVPYATLLTVLMILSCLVQLGPLPVLIPAIIWLYWTGDTT
                     WGTVLLVWSGVVGTLDNVIRPMLIRMGADLPLILILSGVIGGLIAFGMIGLFIGPVLL
                     AVSWRLFAAWVEEVPPPTDQPEEILEELGEIEKSNK"
     gene            29414..32470
                     /locus_tag="PN977_001987"
     CDS             29414..32470
                     /locus_tag="PN977_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HDI6042017.1"
                     /translation="MIPQISQAPGVVQLVLNFLQELEQQGFTGDTATSYADRLTMSTD
                     NSIYQLLPDAVVFPRSTADVALIARLAAQERYSSLIFTPRGGGTGTNGQALNQGIIVD
                     MSRHMNRIIEINPAEGWVRVEAGVIKDQLNQYLKPFGYFFAPELSTSNRATLGGMINT
                     DASGQGSLVYGKTSDHVLGVRAVLLGGDILDTQPLPVELAETLGKSNTTIGRIYNTVY
                     QRCRQQRQLIINNFPKLNRFLTGYDLRHVFNDEMTEFDLTRILTGSEGTLAFITEARL
                     DITRLPKVRRLVNVKYDSFDSALRNAPFMVEARALSVETVDSKVLNLAREDIVWHSVS
                     ELITDVPDKEMLGLNIVEFAGDDEALIDERVNALCVRLDELIASQQAGVIGWQVCREL
                     AGVERIYAMRKKAVGLLGNAKGAAKPIPFAEDTCVPPEHLADYIAEFRALLDSHGLSY
                     GMFGHVDAGVLHVRPALDMCDPQQEILMKQISDDVVALTAKYGGLLWGEHGKGFRAEY
                     SPAFFGEELFAELRKVKAAFDPHNRLNPGKICPPEGLDAPMMKVDAVKRGTFDRQIPI
                     AVRQQWRGAMECNGNGLCFNFDARSPMCPSMKITQNRIHSPKGRATLVREWLRLLADR
                     GVDPLKLEQELPESGVSLRTLIARTRNSWHANKGEYDFSHEVKEAMSGCLACKACSTQ
                     CPIKIDVPEFRSRFLQLYHTRYLRPLRDHLVATVESYAPLMARAPKTFNFFINQPLVR
                     KLSEKHIGMVDLPLLSVPSLQQQMVGHRSANMTLEQLEALNTEQKARTVLVVQDPFTS
                     YYDAQVVADFVRLVEKLGFQPVLLPFSPNGKAQHIKGFLNRFAKTAKKTADFLNRMAK
                     LGMPMVGVDPALVLCYRDEYKLALGDERGEFNVLLANEWLASALDSQPVATVSGESWY
                     FFGHCTEVTALPGAPAQWAAIFARFGAKLENVSVGCCGMAGTYGHEAKNHKNSLGIYE
                     LSWHQAMQRLPRNRCLATGYSCRSQVKRVEGTGVRHPVQALLEIIK"
     gene            32467..32877
                     /gene="menI"
                     /locus_tag="PN977_001988"
     CDS             32467..32877
                     /gene="menI"
                     /locus_tag="PN977_001988"
                     /EC_number="3.1.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA hydrolase"
                     /protein_id="HDI6042018.1"
                     /translation="MIWKRKITLEALNAMGEGNMVGLLDIRFEHIGDDTLEATMPVDS
                     RTKQPFGLLHGGASVVLAESIGSVAGYLCTEGEQKVVGLEINANHVRSAREGRVRGVC
                     KPLHLGSRHQVWQIEIFDEKGRLCCSSRLTTAIL"
     gene            32977..33165
                     /locus_tag="PN977_001989"
     CDS             32977..33165
                     /locus_tag="PN977_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiH family protein"
                     /protein_id="HDI6042019.1"
                     /translation="MSTQLDPTQLAIEFLRRDQSNLSPAQYLKRLKQLELEFADLLTL
                     SSAELKEEIYFAWRLGVH"
     gene            33714..34082
                     /gene="sufA"
                     /locus_tag="PN977_001990"
     CDS             33714..34082
                     /gene="sufA"
                     /locus_tag="PN977_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold SufA"
                     /protein_id="HDI6042020.1"
                     /translation="MDMHSGTFNPQDFAWQGLTLTPAAAVHIRELVAKQPGMVGVRLG
                     VKQTGCAGFGYVLDSVSEPDKDDLLFEHDGAKLFVPLQAMPFIDGTEVDFVREGLNQI
                     FKFHNPKAQNECGCGESFGV"
     gene            34091..35578
                     /gene="sufB"
                     /locus_tag="PN977_001991"
     CDS             34091..35578
                     /gene="sufB"
                     /locus_tag="PN977_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416198.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HDI6042021.1"
                     /translation="MSRNTEATDDVKTWTGGPLNYKEGFFTQLATDELAKGINEEVVR
                     AISAKRNEPEWMLEFRLNAYRAWLEMEEPHWLKAHYDKLNYQDYSYYSAPSCGNCDDN
                     CASEPGAVQQTGANAFLSKEVEAAFEQLGVPVREGKEVAVDAIFDSVSVATTYREKLA
                     EQGIIFCSFGEAIHDHPELVRKYLGTVVPGNDNFFAALNAAVASDGTFIYVPKGVRCP
                     MELSTYFRINAEKTGQFERTILVADEDSYVSYIEGCSAPVRDSYQLHAAVVEVIIHKN
                     AEVKYSTVQNWFPGDNNTGGILNFVTKRALCEGENSKMSWTQSETGSAITWKYPSCIL
                     RGDNSIGEFYSVALTSGHQQADTGTKMIHIGKNTKSTIISKGVSAGHSQNSYRGLVKI
                     MPTATNARNFTQCDSMLIGANCGAHTFPYVECRNNSAQLEHEATTSRIGEDQLFYCLQ
                     RGISEEDAISMIVNGFCKDVFSELPLEFAVEAQKLLAISLEHSVG"
BASE COUNT         9043 a         8615 c         9016 g         8974 t
ORIGIN      
        1 agcagttgga gaatattcag aaagcctggg ctgatcgtgg ttttgtggtg ctgggattcc
       61 cgtgcaacca gtttctggaa caagaaccgg gcagcgatga agagattaaa acttactgta
      121 ccaccacatg gggggtgacg ttcccgatgt tcagtaagat tgaagttaat ggcgaaggac
      181 gccatccgct gtatcaaaaa ttgattgccg cagcgccgac cgcagtcgcg ccggaagaga
      241 gcggattcta tgcccgtatg gtcagcaaag gtcgtgcacc gctgtaccct gatgatattt
      301 tatggaattt tgagaaattt ttagttggca gggacggatt ggtcatccag cgtttttccc
      361 cggatatgac gccggaagat cccattgtga tggaaagcat taaactggcg ttggcaaaat
      421 aatgtctatt gtgatgcagt tacaggatgt tgcggaatct acccgcctgg ggccgctttc
      481 tggcgaggtt cgggctgggg agatcctgca cctggtgggg ccgaacggcg cgggtaagag
      541 taccttgctt gcacgaatgg ccggaatgac cagcggtaag ggaagcattc agttcgcggg
      601 gcaaccactg gaagcatggt ccgcaacaaa actcgcgctg catcgcgcct atctttcaca
      661 acagcagacg ccgccgtttg caatgccggt ctggcactac ctgacactgc atcagcacga
      721 taaaacgcgt accgaactac tgaatgatgt cgcaggggcg ctggctcttg atgacaaact
      781 cggacgtagc accaatcaac tttccggcgg cgaatggcaa cgcgtacgtc ttgctgcggt
      841 ggtgctgcaa atcacaccac aagccaatcc cgcaggccaa ttactacttc ttgatgagcc
      901 tatgaacagt cttgatgttg cgcaacaaag tgcgttagac aaaattctga gcgcgctgtg
      961 tcagcaagga ctggcgattg tgatgagcag tcacgatctc aaccatacat tgcgtcatgc
     1021 gcatcgggcg tggttgctaa aaggtggaaa aatgctggcc agtggacgca gggaagaggt
     1081 gctcacgccg ccaaatctgg cgcaggccta tgggatgaat tttcgccgtc tggatatcga
     1141 aggtcacaga atgctgattt cgaccatctg atttcattag tatcttgaaa atcgggtaat
     1201 tacgacgcta aattaatgca agaataaaaa acagaggaac cgtacggaat gcgtttctgc
     1261 cttattttga tcacagcact gtttctggcc gggtgtagcc accataaagc accgccgcca
     1321 aatgccagac tttctgattc gattaccgtt attgccggtt tgaacgacca gctacagagc
     1381 tggcatggca cgccgtatcg ttatggtggc atgacgcggc gcggtgtgga ctgttcgggg
     1441 tttgtggttg tgacgatgcg cgatcgtttc gatttgcagc tgccccgaga aaccaaagaa
     1501 caagcctcta tcggcacgca aattgataaa gacgagttgc tgcctggtga cctggtcttt
     1561 ttcaaaacgg gctccggaca aaatggtttg catgtaggta tttacgatac caacaaccaa
     1621 tttatccacg cctctaccag caagggagtg atgcgttcct cactcgataa tgtttattgg
     1681 cagaaaaatt tctggcaggc gagacgaatc tagtatgtcg aaaaatggac ggcgatgata
     1741 catctccgtc catttactcc tgaatacagt agtattttac agacaaaaat tccgtaaatg
     1801 gattattcgt ttataatcag gataatctaa taaaaggacc cctgaaaaag tggttaacat
     1861 ggaacaaatg aaagttactc cttctgtaat tgttatgtct tatggcctca tcccttttag
     1921 ccggatactg aaaaacatcc ttcgagaggg acggttacca tgaagatttt tttggagaat
     1981 ctttatcatt ctgattgcta tttcctgccg ataagagata accagcaaga ccttgtcggc
     2041 gttgagttaa ttacccattt ctccagtgaa gatggtacgg ttcgtatacc gacgagtcgg
     2101 gttatagcgc aacttaccga ggagcaacat tggcaattat tttctgagca gttggaattg
     2161 ctaaaatcat gccaacactt ttttattcaa cataaactct ttgcctggct taatttaacc
     2221 ccacaagtgg ctactttatt attagacagg gataattacg cgggcgaact attaaaatat
     2281 ccgtttattg aactgttgat taatgagaat tacccgcatc tcgatgaagg aaaagataat
     2341 cgcgatcttt tttccttatc gcaaatgtat ccccttgtgt taggtaattt gggcgcaggc
     2401 aatagcacaa tgaaagcggt ttttgatggt cttttcaccc gcgtcatgct ggataaaagt
     2461 tttattcagc aacaaatcac gcatcgctcc ttcgaaccgt ttattcgtgc gatccaggcg
     2521 caaatttccc catgttgcaa ttgcatcatc gcggggggca ttgatacgcc agaaataatg
     2581 gcacagatta ccccctttga tttccatgct ctccagggat gcctgtggcc tgccgttcct
     2641 gtaaatcaga taacgacgtt ggttcagcga taaccctcct gtttgctggt gtttaagacg
     2701 agagtaaccg tctacactat caaacaggag gatctatgac cctgtctttt attacccgct
     2761 ggtgcgatga attgccagaa acctatacag cactttcccc tacgccttta aataaggccc
     2821 ggctgatttg gcataatgcc gaactggcta acacgctgag tattccatcg tcgctgttta
     2881 aaaatggcgc aggtgtctgg ggcggcgaaa ccctactgcc tggtatgtca ccactggcgc
     2941 aggtttacag tggtcatcag ttcggcgtct gggcgggcca actgggtgat gggcgcggca
     3001 ttttactcgg cgaacaacag ctggccgatg gcactacaat ggactggcat ctgaaaggtg
     3061 ctggcctgac gccttattcg cgaatgggtg atggacgggc agttttacgt tcgacgatac
     3121 gagaaagtct cgccagtgag gcgatgcatt atctgggcat tccgacgacc cgcgcgttaa
     3181 gtatcgtctc cagcgattcg ccagtgtatc gggaaacggt ggagccaggc gcgatgctga
     3241 tgcgtgtggc accaagtcat ctgcgctttg gtcatttcga acatttttac tatcgccgcg
     3301 agccggaaaa ggttcgtcag ttggctgact ttgccattcg tcattactgg tcacatcttg
     3361 cagatgatga ggacaaatac cgtctctggt ttagcgatgt tgtcgcacgt accgcatcgt
     3421 taattgccca atggcagacg gtcggctttg ctcatggggt gatgaatacc gacaacatgt
     3481 cgctgctggg gctgacgctt gattacgggc cgtttggttt tcttgatgat tacgaacccg
     3541 gttttatttg taatcactcg gatcatcaag ggcgttatag ctttgataat caacctgctg
     3601 tcgcgttgtg gaatttacag cgtctggcgc agacattgtc accatttgtt gccgtagatg
     3661 ccctgaatga ggccctggac agctatcagc aggttttgtt gacgcattat ggacaacgga
     3721 tgcggcagaa gctgggcttc atgacggagc aaaaagagga taacgcgcta ctgaatgaat
     3781 tattcagtct gatggcgcga gagcgcagcg attatacccg cacattccgc atgctgagtc
     3841 tgaccgagca gcacagcgcg gcgtcaccgc tacgtgatga gtttattgat cgtgcggcat
     3901 ttgatgactg gtttgcccgt tatcggaggc gtttgcaaca agacgaggtt agcgatattg
     3961 agcgtcagca actgatgcaa agcgttaacc cagctctggt gttgcgcaac tggttggcgc
     4021 aacgggcgat tgaagcggca gaaaagggtg atatgacgga attgcaccgc ctgcatgagg
     4081 cgttgcgaaa tcctttcagc gacagagatg atgactatgt cagtcgtcca cctgactggg
     4141 gtaaacggct ggaagtcagc tgctcgagtt aaaggctact tggtcaacag cagcttgcca
     4201 gcctgagttt tacggagcag atattcttgc ccgtcatgat cgataatcag tttgccatcc
     4261 ggacctaaca gtgtctggct ggtaattcgc cgaatgggct gcggggaagc ggtcttgtga
     4321 ttggcatccg tttcaggcgt gaattttctg ctatccgtat aacgcataaa aattaataat
     4381 aattgatata acaatgattc ttattatcaa tattctggcg ggccagagca agcagtaatg
     4441 tgttgagaag acaacaagtt gcgcagaaat gcccattccg tcaggaatgg gcacgcattc
     4501 agaagcgggt atcaaccgca gaggcgagtt tttcgaccag gcgttcggta tcttcccagc
     4561 ccagacacgg gtcggtaatc gattgaccgt aagtgagcgg ctgaccgccg acgatttttt
     4621 gcgttccttc gcgcaggaaa ctttccgcca taattccagc aatcgccgta gagccattgc
     4681 ggatttgctg acaaatatcc tcacaaactt ctaactggcg acggtgctgc ttctggcagt
     4741 taccgtggct gaaatccacc accagatgtt caggtaaatc aaactcgtgc agcgtatcgc
     4801 aggctgcggc gatatcatcg gcatggtaat tcggtttttt gccgccacgc ataataatat
     4861 ggccatacgg gttgccactg gtctgataga tggtcatctg accgtttttg tctggtgaga
     4921 ggaacatatg gctggcgcgg gctgcgcgga tagcatccac ggcaatccgc gtgttgccat
     4981 cggtaccatt tttaaaacct accggacagg agagtgctga agccatttcg cggtggatct
     5041 gactttcggt agtacgagcg ccaatcgcgc cccaactgat taaatcagca ataaactgac
     5101 cggtcaccat atcgaggaac tcagtcgcgg tagggacgcc cagctcattt acctgtaaaa
     5161 gtaatttgcg cgccagctcc agaccgtgat ttacccgata gctaccgttt aaatctggat
     5221 cggagatgag tcctttccag ccgacaacag ttcgtggttt ttcaaaatag gtgcgcatta
     5281 cgatttccag ccgtgactgg tactggttgc gcagcgactg cagacgggtg gcgtactcca
     5341 ttgcagcggt gagatcgtgg atcgagcagg ggccaataat gaccaacagt cgcttatctt
     5401 caccattcag tattttttca attctgcggc gggagtcggt gacatgggtg gcgacgccag
     5461 gcgttacggg ataccgtagc gcgagttcgg cgggcgttac caggctctca atacgcgcag
     5521 tacggagttc gtcagttctg ttcattacaa gtctcagaaa tttgttgctt ctacggcagg
     5581 actaccggga agtgatgcgc atcacgataa accaatcgca gctgatttca agtgcgaggt
     5641 gtgtcacacc tcgccgggtg gttagcatga taacaaaaaa ataagttaat gcactagttc
     5701 tctagtacat tcgacggcta aggcccatga tatcgaggat cttggtggca atctcttcta
     5761 ccgaataatt ggtactgtta atccacggga tctgattttt acggtacaac gcttccactt
     5821 ccgcgacttc catcctgcac tgacgaagcg aggcatagcg actgttctcc cgacgttcct
     5881 cgcgaatcgc cgccagacgt tccgggtcga tagtcaggcc gaacaatttg tgctgaagcg
     5941 gtttgagcga cgcgggtagc accagattat ccatatcgtc ggcaataaag gggtagtttg
     6001 ccgcgcggat accaaattgc attgccagat acagactggt gggggtttta ccacagcgag
     6061 aaacaccgag caggatcacc tgagcctggt ccagattgcg caacgaaatg ccgtcgtcat
     6121 gggcaagggt gtaatcaatc gccgcaatac gcgcatcata tttattgagg ttattagggt
     6181 tcaggccatg ggtacgatga gcaatcggcg tgggatccag tttcatctct tgttgtagcg
     6241 gggcaaccag cgcctgaacg atatcctggc aaaagccctc actttgcaag atgatggcgc
     6301 gaatctccgg caacacgatg gagtagaaga ccagcgggcg cacgcctgtc tggtgataaa
     6361 ttgcgtcaat ctgatccttc actgcccgtg cacggctctc attttcgaca aacggcagcg
     6421 taatgctgct gatagtgacg ggaaattgtg acattactgc gtgtcctaat acctccgcag
     6481 ttattgccgt accatcagaa atataaaaaa cgtggcgatc aacagcatta tccattttgt
     6541 tcttcccgtg atgcagacat aattctatga aagcataaat taaaaacgca cagaagcgta
     6601 gaacgttatg tctggtttat aaatgaacct tcaattttat tttttatgaa aacagcattt
     6661 catttttatg gtttcgttta taccgatggt ttatgtggaa attgtcgaag agagcagatt
     6721 tgcgcaacgc tgggatcagt cttaaaaagt aaaaaaatat atttgcttga acgattcacc
     6781 gtttttttca tccggttaaa tatgcaaaga taaatgcgca gaaatgtgtt tctcaaaccg
     6841 ttcatttatc acaaaaggat tgttcgatgt ccaacaatgg ctcgtcaccg ctggtgcttt
     6901 ggtataacca actcggcatg aatgatgtag acagggttgg gggcaaaaat gcctccctgg
     6961 gtgaaatgat tactaacctt tccggaatgg gtgtttccgt tccgaatggt ttcgccacaa
     7021 ccgccgacgc gtttaaccag tttctggacc aaagcggcgt aaaccagcgc atttatgaac
     7081 tgctggataa aacggatatt gacgatgtta cccagcttgc gaaagcgggc gcgcaaatcc
     7141 gccagtggat tatcgacact cccttccagc ctgagctgga aaacgccatc cgcgaagcct
     7201 atgcacagct ttccgccgat gacgaaaacg cctcgtttgc ggtgcgttcc tccgctactg
     7261 cagaagatat gccggacgct tcttttgccg gtcagcagga aaccttcctc aacgttcagg
     7321 gttttgacgc cgttctcgtg gcagtgaaac atgtatttgc ttctctgttt aacgatcgcg
     7381 ccatctctta tcgtgtgcac cagggttacg atcaccgtgg cgtggcgctc tccgccggtg
     7441 ttcagcggat ggtgcgctcc gacctcgcat cttctggcgt gatgttctcc attgataccg
     7501 aatccggctt tgaccaggtg gtgtttatca cttccgcatg gggccttggt gaaatggtcg
     7561 tgcagggtgc ggttaacccg gatgagtttt acgtgcacaa accgacactg gcggcgaatc
     7621 gcccggctat cgtgcgccgc accatggggt cgaaaaaaat ccgcatggtt tacgcgccga
     7681 cccaggagca cggcaagcag gttaaaatcg aagacgtacc gcaggaacag cgtgacatct
     7741 tctcgctgac caacgaagaa gtgcaggaac tggcaaaaca ggccgtacaa attgagaaac
     7801 actacggtcg cccgatggat attgagtggg cgaaagatgg ccacaccggc aaactgttca
     7861 ttgtgcaggc gcgtccggaa accgtgcgct cacgcggtca ggtcatggag cgttatacgc
     7921 tgcattcaca gggtaagatt atcgccgaag gccgtgctat cggtcatcgc atcggcgcgg
     7981 gtccggtgaa agtcatccat gacatcagcg aaatgaaccg catcgaacct ggtgacgtgc
     8041 tggttactga catgaccgac ccggactggg aaccgatcat gaagaaagca tctgccatcg
     8101 tcaccaaccg tggcggtcgt acctgtcacg cggcgatcat cgctcgtgaa ctcggcattc
     8161 cggcggtagt gggctgtggt gatgcaacag aacggatgaa agacggtgag aacgtcactg
     8221 tttcttgtgc cgaaggtgat accggttatg tctatgcgga gttgctggaa tttagcgtga
     8281 aaagctccag cgtagaaacg atgccggacc tgccgttgaa ggtgatgatg aacgtcggta
     8341 acccggatcg tgctttcgac ttcgcctgcc tgccgaacga aggcgtgggc cttgcgcgtc
     8401 tggaatttat catcaaccgt atgattggcg tccacccacg cgcgctgctt gagtttgacg
     8461 atcaagaacc gcagttgcaa aacgaaatcc gcgagatgat gaaaggtttt gattctccgc
     8521 gtgaatttta cgttggtcgt ctgactgaag ggatcgcgac gctgggtgcc gcgttttatc
     8581 cgaagcgcgt cattgtccgt ctctctgatt ttaaatcgaa cgaatatgcc aacctggtcg
     8641 gtggtgagcg ttacgagcca gatgaagaga acccgatgct cggcttccgt ggcgcgggcc
     8701 gctatgtttc cgacagcttc cgcgactgct tcgcgctgga gtgtgaagca gtgaaacgtg
     8761 tgcgcaacga catggggctg accaacgttg agatcatgat cccgttcgtg cgtaccgtag
     8821 atcaggcgaa agcggtggtt gaggaactgg cgcgtcaggg gctgaaacgt ggtgagaacg
     8881 ggctgaaaat catcatgatg tgtgaaatcc cgtccaacgc cttgctggcc gagcagttcc
     8941 tcgaatattt cgacggcttc tcaattggct caaacgacat gacgcagctg gcgctcggtc
     9001 tggatcgtga ctccggcgtg gtatctgaac tgttcgatga gcgcaacgat gcggtgaaag
     9061 cactgctgtc gatggcgatt cgtgccgcga agaaacaggg caaatatgtc gggatttgcg
     9121 gtcagggtcc atccgaccac gaagactttg ctgcatggtt gatggaagag gggatcgata
     9181 gcctgtctct taacccggac accgtggtgc aaacctggtt aagtctggct gaacttaaca
     9241 agtaaataaa aacccggcgg cgttcagtcg ccgggtccat ttctatatcc ccacctttta
     9301 acaacctgct ttgggcaaac gcgctcatgc aatataaaaa gtttccggac atcatctgaa
     9361 taattggctg ctgttctttc tgattttaat ttgtttgcga tcctctccac agaataattc
     9421 tcgataccgt tttttctcaa agccgaatgt gaccacacgt caatttcctt aagcatatgc
     9481 gtaatttttt cgtccagagt tcgttggatt gcacgaagtc actgatgagt cccgttttta
     9541 tactatcttc aaccacagca taaaaaggga tatggccatg aaaaattacc gcaccgttat
     9601 tcagacataa gcgcagatta tgtaattcag agaatttatt cgaactaaaa ccgattttct
     9661 gatttgtctg gctgtacaga aaacaggata aattccctcc gcgagcggtg ggaaacagct
     9721 acagggattg catatgagtt gctatttgaa agtcctgatt tgtgcgattg tcgcactcgc
     9781 tatatggttt ttccccgtcc ccgacggatt aacaccctta acctggcaca ttctggccat
     9841 atttatcacc actgtggtgg cgtttattct gcaaccgttg ccggtgggcg ctatcgcgtt
     9901 aatagccatc agttttatta tgctgaccgg tatgatgaaa accagcgaag ccttaaaagg
     9961 tttcagcagc acgacggtat ggcttattgt cgccgcattc ctgtacgcga aaggattcat
    10021 caaaacgggg ttggggcggc gtatcgccta tttgctgatt cgcggatttg gcggcagctc
    10081 gttgcgtctg ggttatacat tggcgctaag cgatatgatt atcgcgcccg caacgccatc
    10141 aaacacagcg cgagcaggag ggattttgtt cccgattgtt cgcagtgtct ctaacagctt
    10201 tggatctgaa ccggatcaag ggccgcgtaa aataggcgct tatctgatgc agacggtgtt
    10261 ccattccaac tgtctctcat cctcgatgtt tatgacggcc agcgcgccaa atgcactcat
    10321 tgtatctctg gcggccagca ctttatttgt cgatatctca tgggggatgt ggacgctaag
    10381 cgcactggtg ccgggcatta tcgcttttat cactatgccg ttggtcattt acaaactcta
    10441 cccgccagag atcaaaaaga cgccagaagc caaagcgttg gcacaggaag aacttatcaa
    10501 aatggggccg gtaacccgtg atgagcgcgt gacaattggc attttcctgc tttcattatt
    10561 ggcctggagt acatcgaaat ggactggcct ggatgcgacg gctgtcgcgc tttctggcgt
    10621 ctgtttaatg ttgatgaccc ggattatcac ctggcaggat gtgcagtcag agaagggggc
    10681 ctgggacatt ctggtctggc tgggagtcat gatctgtatg gcagataagc tcaatcagct
    10741 tggcctgttc aaatggtttg ccgtcacaac ctcggcgctg ttcaccggca ttccgtggga
    10801 gataacgttg acggtgttgc tgatagttta ctgctattcc cactatttct ttgccggaag
    10861 tacgccgcat gtagtggcga tgtatgccgc ttttggcagc gtctcggttg ctgctggcgc
    10921 accgccgatg atggcggctc tgtcactggc gttcgtcact aacctgatga gcggcatttc
    10981 gcattatggc aacggacccg cagtgatcta ttacggcgct ggctatgtct cgcaacgaga
    11041 atggtggcgg ctgggcttta tcgtcatgtt gttgaacatt gccatctggt tcggactggg
    11101 ggcggtgtgg tggaaaattc ttggcctgtg gtaagtggcc tgcgacaaac atcaatcaag
    11161 gaatagtgta tgaacttaaa agatattcaa gtggcgatcg tcggcaaatt aagtgaccgc
    11221 ttaatggcta aagttgaatc taccttcacc gcatatcgcc tgtgggaact ggctgatgaa
    11281 ggcgcttttt acacgcaaat cgcgccggat attcaggcgc tggtgaccag tggtaatccg
    11341 gtaatggggg cttctcgggc gctaatagaa aaatttccga atctgaagat tattgccagc
    11401 aacggtgtgg gttatgaccc tattgatatc gtggcagcca gggagcatgg cgttatcgtc
    11461 accaacacac ctggcgtgct taatgactgt gtcgcagaca ttggtatagc gttgttactc
    11521 aatgttgccc gacgtattaa cattgccgat tgttatgttc gtgacggccg ttggccaaac
    11581 gaggggcgtt tcccgatggc gacgaaagtg agtggcaaac gttgcggaat tgtcggtctg
    11641 gggaacattg gtaacgcagt agcccgccgt gcagcagcat ttgaaatgga aattcactat
    11701 ttcgacccaa aaccgcataa caaacctgat tggacggcgc atgattcgtt ggtttctctg
    11761 gcgcaagcgg tggacttcct cgttctgacg ctgccaggcg gcgcgagtac gcgcggcgtt
    11821 attgacaaaa cggtgctgca ggctctgggg aaaaccgggc atttgatcag tatttcccgg
    11881 ggatcagtgg tgaacgaaac cgatctgatt gatgctctgc aaaataacat cattgctggt
    11941 gcggcactgg acgtttatgc caacgaacca catgtgccag aacaattaat gtcgctggat
    12001 aacgttggcc ttacgccaca tatcgccagt ggtacgagcg aaacgtttaa tgcgatggcg
    12061 gatttggtat ttgataactt acaggctttc tttagcggac ggccagttat tacccctgtt
    12121 actcaataag gcggaaaata tgatttatgg tgatatcgcg tatcttagtg aacaacaaaa
    12181 gcggacattt catcctgtca tcactcgtgt cattgagtat ctttcacaac atgatctgaa
    12241 tagtcttcct gtgggagaga aggtttttat tcaggatgac tcaatatttt ataccgtggt
    12301 tgaaccgatg actaaaccac tacatgaatg tcgaccggaa gttcatcgta aatatcttga
    12361 tgtccatttt ttaattgccg gagaagagcg gatttgtgtg gattttgatg atggggaaaa
    12421 ctcggttgta gaaagccacc tgggcgaccg tgatgttatt ttttatgctg atgaacgtat
    12481 taatgatcga atcatcacgc tgacaccagg acagtttgtg gtggtctttc cggaagaagt
    12541 gcatcgggca tgctataaaa tcgataacgt ggaaaaaatc aaaaaaacag tcgtgaaaat
    12601 cgacatgagt ttattatagg cggagctggc tatgacaaaa attgccctgg tgacgggggg
    12661 aagtaaaggc atcggctacg cagcagcaaa ggcactctat gacattggat atacagttat
    12721 tgttgccgcg cgtaacgaag aaaccttaca gcaatgtgca caaggattag ccaaggaacg
    12781 ctttattcct ctggttgccg atgtcacgga tgagtccgct gtcgttgcgc tatttgccga
    12841 gattaaggct ctttttggtc gactggatct cttgttcaat aatgcgggca ataatagtcc
    12901 ggcaaaaagt attgaagagg ttgagtttac tgagtggacg cgcgtgttta acgccaatat
    12961 tcattcgaca tttttgtgtt cgaaagaggc gattaaaatc atgaaagcgc agcagccaat
    13021 gggaggacgt attattaaca atgcgtcgat ttctgcactg acgccgcgcc tctatactgc
    13081 gccatacacg gcgtcgaaac atgctgttac cgggttgacc aaggccattt ccctggattg
    13141 tcgcaaattt aatatcgctt gcgggcaaat taatatcggc aatgcggaaa cgacattatc
    13201 gaatcggatg cgtaagggcg tttatcaggc agatggtgct attaaagcgg aagatatgat
    13261 gaatgttgct gatgttgcag cgaccattca gatgatggct cagttgccag taaccacgaa
    13321 tgtgctggaa ataacgatta tggcaaataa tatgccgtat gtcggacgtg gttaatcgag
    13381 attgaataaa gcaccttgcg tgtaaggtgc tttattattt atgacttatg cctcaacgca
    13441 tgtttgttcc agccgttgaa gaatatcttt acgtaacaaa aatttttgta ttttaccgga
    13501 ggcagtgcgc ggtagttttt cgattaccac gatatgttca ggatatttat atttcgcgac
    13561 ccgtttacgg ctaaaaaatg ccactacatc ttccagcgat aatgaatgat gcggtgcttt
    13621 cagcacgaca taagcgcatg aacgttcacc taagcgttca tcgggcatcg caaccacaca
    13681 agcatcgtga attttaggat gctgtaataa aatatcttcc acttcacggc tgctaatatt
    13741 ttcgccgccg cggacaataa tatctttctt gcgcccggtt atttttatat agccagcctc
    13801 atccatgcgg cagagatcgc cgctgtaata ccagccttct tcatccaggg catgggcggt
    13861 taattcaggt tcatcaaaat accccataaa cacattgggg ccacgcgagg cttcttcacc
    13921 ttcgcaacct ggcggtaagg tcttgcgtgc gccatcgacc actttaatct ctacacctgc
    13981 ggcagcgtaa ccatcggtgt gcataaagcg cgacaaagga tcatcgagat tcaccaccgc
    14041 atgcggcgaa ctttctgtgg aaccataaac acttaataat ttaatgccgc gctgctggca
    14101 ttcacgcgcc acttttttgg ggattgtggt accgccacaa agaaagaaac gcagcgctga
    14161 aaggtctgcg ggctgtttct ctagtaaatt caaaagatca tagacaaacg gcgttgcgcc
    14221 gagcatacag gtgcaacgct gctgctcaag cagcgcgaga cacgcagcag gagtgaaaat
    14281 atctaacaac acgctgcgag caccaattaa aaatggtgct gttacgccat gcagaaagcc
    14341 cgttgcgtga ccaagtggcg caggcatcat aaagacatcc tgccaggtca gattcagccg
    14401 cgcgcaataa gcccgctcac tggcgagaat attgttatgc gttagcatca cgccctttgg
    14461 cagaccctcg gttccggagg taaatagcac agcggctaat tcatcgccgt gggtcgttat
    14521 cgccgtagtc agtggggtat tgtcggcgag aatctgactt aatgagaggg aagaggtggc
    14581 gggagccagt ttgtccacgc cgacaatttg ttgtagttgt ggaagctgat tttgcagcgg
    14641 caggattaaa tctaccggac gcgtttgttt aaacaacgtc ggtgcaaaga acatttttgc
    14701 ctgacactta ttgagtaccc atactagttc tgcttcccgc caggaaggca acagcggtac
    14761 ggaaaccgca ccgattttca ggcaggcaag atagataacg gtaaattcac accagccagg
    14821 cagttgaaat gcgatgcgat cgcctgattc aatacccttc gtcaacatcc agtttgccag
    14881 acagctcgcg gcgtgatcga gcgcgctata ggtgtatgat gcaccatgat tatcgaccac
    14941 ggcaattttg tctggcatcg cacgagcggt ctgctgccag taatcggcca gcgaagcatc
    15001 gccccatagc ccttgctgac gatacgccgc acgacgttgt tcgttaaacg ttaatgtcac
    15061 tttcattttg gactctcgaa acagaacatc aggcccgaga tgcgggcctg tgggatgcat
    15121 gattagccgt agcgaaaatc aataccgaag gtgcctgcgg gatattgcca ctgttcgaga
    15181 atagtgttgc cgcacagcac ccgacaggtg ccgcactcca gacaaccggc ggaatcgaaa
    15241 tgaatgtttc ctgcgtcatc ctgcttataa agtccggcag ggcaagcttt cattaattta
    15301 cggaattcat tgatatcggg atttgccgcc aaaatgatat gcgggtgtcc ctcatcaaca
    15361 tggaatttat tgacgcctaa tttgatgtcg acgttaaccg tagcgttctg gctcatagcg
    15421 cggttgctcc cttaatgcca tctttcagca agttgatcag cccaattttc ttcgcgtgtc
    15481 ccatgatcat tttgcgtacc ggctggtttg gtttgccgtc aatggtgaac atctcgttca
    15541 tgatgtcggc gaccatgcgc ggatactgac taaacaggcg cggattttcc atcagcgccg
    15601 ggatcttgcg aaaatgctgc atatcacgca tgacgcagct ttgttccagc tcacgtttgt
    15661 attgcgccag actgctggcg gagaaatccg cgcgttcttt ggctgcaatc actgttgtgg
    15721 cggcagcctg agctgatgca atggctaaat ccatgccgcg aactgtaaaa cccaaattca
    15781 ggcagaagcc tgcggcgtca ccaacgatca tcacgccatc gttaaccagc tgcggcacca
    15841 ttgccagacc gccctccggc accatatgcg cggaatattc aagcagtttg ccgccgctaa
    15901 tcagcgggtg aatggcgggg tgttgtttaa aatcttccag catttgcggc acgctttttt
    15961 gcgcatgggc gatatcaccc aatccacaaa ccagccccaa cgatatggaa tccttattgg
    16021 tatagaggaa tccgccgccc atcaggccgt cagaagggga accggcaaac agccaggcgg
    16081 caccttcctc gcccgtaata ttaaagcgat ccttgatctg ttctggtgtg aggccaataa
    16141 cctctttaac accaacagcg taatgatgcg gatcggaagc gggaaccatt cccagcgagc
    16201 ggccaagcat cgagttaacg ccatcagcca gaatcaccac attcgcttcg agaatatcat
    16261 ccccggcctg gacgccagtg accttatttc cttcacgaac cagcgcatcg acacgaacgc
    16321 ccgggataaa ctgcgcgcca gcctgttcgg cttgttccat caaccacggg tccagacgat
    16381 tacgcaatac ggtataagac gcgtgttgcg gaacatctgg ttgctcgcgg tgaaaatcga
    16441 gggtaacggc actctcatcg gttaagaagg agattttctc gcgtgtgacc ttgcgttcta
    16501 ccggcgctga tgctgcaaag cctggaataa tcgcttcaag tgtgtgggca taaagacgcc
    16561 cgccggtcat gtttttacat ccggcactgt cgccgcgttc tatcaccagg acatccagcc
    16621 cggcttgcgc catgaccagt gcggcaacgc taccggcaac gcccgcaccg accacaatgg
    16681 catcaaattt gtcatccgac atgcgttatg ctcccttccc gctgcgttta cagcgggaga
    16741 tgtggttgaa agggaattag cgactcaact ggctaatcag ggcagggacg actttgtaga
    16801 tatcgcccac cagaccgtag tcggcatagt tgaagattgg cgcattttta tctttattga
    16861 tggcgacaat cacttttgcg ccgttgccgc caaccatatg ctggatctgc ccggagatcc
    16921 ccagcgtcag gtagagatcg gatttcagca acacgccgga gacaccgata taacgttcac
    16981 gctccatcca gttctcgcct tcggcaattg gacgtgaaca gccgacttca gcattcagca
    17041 ctgccgccag ttcgtggacc attttcaggt catcctgcgc cgccagacca cgaccgacgc
    17101 caaccacacg ttttgctttg ctcaggtcca cgctgcttgc cgctttcgca cggcgttcgc
    17161 gacagagaat ttcatgacgc ggggcaacat aaggtaccgt ttctgtcggg cactgatgag
    17221 aggtatcacg ggtgcacggt tcctgaacac cgggtgcaag ggtaatgatc gccagcgggc
    17281 tgttgatctt ttcctgtgcg aacgctaacc cgccatacat ccggtgttcg gcgcaaatgt
    17341 gaccatcgac aatatccacc gccgtggcat cgttcaccag cgccgcattc agttgcacac
    17401 ttaaccgtgc tgccagagct ttaccacgtt tcgtcgcggc caacagcaac atagcggggt
    17461 gtttatcttt cagcagggca gcaatgcttt cggcgtaatt ttcagtacgt tgcagcgcgt
    17521 cgttttgctc aagaacataa atgcattttg gcccataagg cataactgcc tgcgcctgct
    17581 cggtattttg tacaatggca tacacctgtt ggccccattg ctgagcgccg ccaaacagtt
    17641 cagcataacg ttcaggatta tcgctaaaga cccagacgct gtttaattga ctcatcgttt
    17701 cttctccgta gacttcagtt cagggctttc ttcagatgtt ccgccagctc ggcaatggcc
    17761 tccggcgaat cgttatcgag aatgatgtgc ttacgttctg tttgcggcgg tacgcgaata
    17821 ccggtcagtt cagcaagtgg tgcgctctgg ctccagccaa tgtcacttgc ctgccactga
    17881 tttaccggtt ttttacccgc gccgagaatg gctttcatcg aaggaatgcg tggcatgtta
    17941 atatcggagg tgacgcagag cacggctgga acagagagct caataacttc aacatcatct
    18001 tcaagcgtgc gttcaatcac cagtgtattg ccctgacgct gaatagcact cactgcatta
    18061 atcaccggaa gttgcagaat ttctccgacc agcaagccaa cctgctgggc ataaaggtcg
    18121 ccggaacctt caccaaagat cagtaaatcg aagccgatct tttcaactgc tgccgccagc
    18181 gcctttgcgg tatcgagagg cagtgcatgt tcaagttgcg catcctgcac caaatacagg
    18241 ctatgcggcc cgcgggatag cacgtctttt cgcactttcg agttctgcaa caatgagccg
    18301 ccaatggtca gcgcggctat ctcatcgtca tctgatgcaa gctggcttgc agcttcaatg
    18361 gcattgagat cgaactggct gattttggcg tcggcattgt cgaaattcag ggtatattct
    18421 ggagtgacaa caatgtcctg ttcttcaggc accagcttaa agcaggttat tattttcatg
    18481 atttctcctg tgagtcgcat acggaaccat ctccgatgga aggtaaatat ggcctgaaaa
    18541 tagtgtgcga tagcggtgcg gggttttcga cggagcaagc tgttttcctt gccaacaatc
    18601 ttgcaaaaaa aatgcgatcg ctaaaacaaa agtgcaattt gcggcaatat agttattcca
    18661 tgaccgtaca atcttgcatt cagtgtaaat aatcttgttt agctctcgct aaacgcggtt
    18721 gtataaccgc aatacacgcc gtattttatc cggcatatat tgtttttgct tgctcatgga
    18781 atagggaggt tgtcatgtat caacgctgtt ttgataatgc cagtgaaacg ctgtttgtcg
    18841 ccggtaaaac gccacggctt tcacgtttcg catttagcga tgatccaaaa tgggagtccg
    18901 gacatcacgt tcatgacaat gaaaccgagc tgatttacgt caagaaaggg gtagcaaggt
    18961 ttaccatcga ttcttcgtta tatgtcgcgc atgccgatga cattgtggtg atagaacgtg
    19021 gcaggctgca tgcggtggcc tctgacgtta acgatccggc aacgacgtgt acctgtgcgc
    19081 tgtacggctt tcagtttcag ggggctgagg aaaatcagct actgcaaccg cattcttgcc
    19141 cggtaattgc cgcagggcag ggaaaagaag tcattaaaac cttatttaat gagctaagtg
    19201 tgattttgcc gcaaagtaaa aatagccaaa catcttcgtt atgggacgca tttgcctata
    19261 cgttagcaat tctttactac gaaaacttta aaaatgctta tcgttcggag cagggatata
    19321 ttaaaaaaga tgttctgata aaagatattc ttttctatct gaataataac tatcgcgaaa
    19381 aaatcacttt agagcagtta tcgaaaaaat ttcgtgccag cgtcagttat atctgccatg
    19441 aatttaccaa agagtatcgt atttccccta ttaactatgt tattcaacgg cgtatgacgg
    19501 aagcgaaatg gtcactcact aatactgaat tatcacaggc agagatttcc tggcgtgtgg
    19561 gttatgaaaa tgtcgatcac tttgccaaac tgtttttgcg ccatgtcggc tgttcgccca
    19621 gcgattaccg caggcaattt aaaaactgtt ttgcggaaca agaaatccta tctgaatttc
    19681 ctcaaccagt aagtcttgcc ggataaatct ggcggcgcag tttgttaaga agctgcgccg
    19741 cctgcagatt atttgttctg ataatctttc aggatctgcc gacccgctac gtaaatcata
    19801 atttcgtctg taccgccgcc gatacgttca caacggacat cacgccagaa gcgggacacg
    19861 cgcgcctcat cggtatagcc cagaccgccc atgatttgaa tcgcatcatc aatgacttcc
    19921 attgcggtac gtgcgcagta cagttttgcc agcgccgcgc tggtgcgcag tgactgatgc
    19981 tgatcggctt gccatgccac tttcaacacc atgttgcgca tgttgtcgat cttaatcgcc
    20041 atcagcgcca gtttttcctg gatcatctgg ttatgaccaa tgggcttacc aaaagcgata
    20101 cgctggttgg catagcgggc ggcatcttcg aatgcgcatt cggcaaagcc ggtgctgcgc
    20161 gcggcgttga tcaggcgctc catctcaaag ttgtacatca cattgaggaa acccattcct
    20221 tcttcgccca ccatgtcgct ctcttcaact tccacgttgt cgagatagac ttcgcaggtg
    20281 ctgagcatat gccagccgat tttatgcagt ggattgattt taatgccggg tttactggag
    20341 tcgacccacc acagagtaaa ggcctttttc ggatctttcg gttgcggatc gcgcgccaac
    20401 accagcatat atggatactc tttcgcaccg gtaataaagg ttttctgtcc gttgatgtaa
    20461 accttgccgt ttttacgcgt gtaagtggta gtggcactgt tgttgtctga gcctgcgcct
    20521 ggctccgtca atgccagggc ataggcggga tcacccgttt ccagtgtgct ctctgccgtt
    20581 ttacgtagct gctctgcaga accgaaacgg cgcatactgt gaatacattg accgttggta
    20641 atcaaaaatg ccggagcacc gcattttgac acttccatca gcgccagcat ttgggtgacg
    20701 taatccgcag ggataccacc aaattcttcc ggcacgccaa gcatggaaat accgttatcc
    20761 gccagtgccc gcataaactc acgcgggtat gtcccgtttt gatcgcaggt gcggaaatac
    20821 tcttccggaa agttagtcgt aatcagttcg cgaatactgg ccagcagcag ttcttgttct
    20881 tcagttaaag aaaaatccat cattatactc ctgttaatga gccgcttcag gcaggacaaa
    20941 acccatcgcc gcatcgataa ataatctttc gtccatcagt ttgagttctg gcgaaatcac
    21001 tggggtgaaa tccattttgt cgaaaatatc tttttgtaaa tcgacgccag gagcgatttc
    21061 gattaaatgc aggccgtctt cttttaacgt gaatacggcg cgctcagtga tataacgaac
    21121 atccagccct cgctcgagag cgatttttcc gctgaaagtt atttccggta gttcccgaat
    21181 aaatttcttc acccgtcctt cctggacgat atttaatttg ccgtcggtaa tttctgtttt
    21241 taaactgccc gcagttaatg tgccgcagaa cacgattttc ttcgaagtgg cactgatatc
    21301 aataaatcca ccggtgccca tgattttacc attgaattta tgcacgccga cgttaccgtg
    21361 ctggtcgact tcagcaaaac tcaaataaca aacatccaga ccaccaccgt gataaaaatc
    21421 aaactgggac gtcatatcca gaatggcgcg ggtattcacg ttcgcgccaa aggcgatccc
    21481 ctgtgaagta ataccgccaa tcggacctgt ttctaccgtc agaataaagt catcagcaca
    21541 accttcttcc cgggcgacca ggccaatgcc gtcagcaata ccgacgccga cattccccac
    21601 tgcgcctttg cgcatttcga ataatgcgcg ccgcgcaact aatttacgtt ggtttagggg
    21661 caggctaagt ttggtactgt catcaagggt gaagtcacca gaaataaagc ggttaaccgg
    21721 tgcaccgcca tacagttggg tttgatccgg atcgaccacc acaatatcca ccagataacc
    21781 cggaatacgg acagatttag gatgcagcgt ggctttctta accattttct gcacctgcat
    21841 catcacaata ccgccgttat tgtgcaccgc ctgagcaata accaatgcgt cgagatacat
    21901 cacctcatct tcaaaagtgg cgtagccttc gctgtcgcag gtggtagcgc gaatgaaggc
    21961 gatatctggc gcaatcgatt tgtaatagag atattctttg ttatcaaact cgaccagttt
    22021 aatcaggtct tctttagtga cttcattcag tttgccgcct tgctggcgtg gatcgacaaa
    22081 tgttccgatg ccaatatcac taataatacc aggctggtgg gctgcggcgg cgcgtaaggt
    22141 ttgtgtaagt acaccttgtg gatagttata agcaataatt ttattttgtt ctgcgagatc
    22201 agaaatacgc ggcgattgtc cccagtgacc gcataatgcc catttcacca gaccttcttg
    22261 tgccagcgga ctaatgccac ggtcggcgcg atcgccaagc cctgttggac taataatcga
    22321 taaattacgt ggtgtttgag tctgtttata tttatcagca agagcagtaa ttaacgtggt
    22381 ggcttccaga ataccgccgc cagcgcctaa cacacaaagt gttgcttcgt cgggaatata
    22441 attcaccgct tcctgtgccg acaggaccgg cactcgtcca ttaatacgag gtggttttac
    22501 aggtttcatt gttaattcct gattcaggtg tgattttccc tacgccagat ggagaattag
    22561 cgcacagaga ctcacgaaat atttttatta aatacggtta aaaatacttt ttttagttcg
    22621 gcgggggagg gtattcccgc cgaaatatta ttgcttatgc ctgatgtaaa atagttaata
    22681 ccgtacgcaa atcatttacc gagatttgcc ctggcgcaga ggcttttttt accgcaccaa
    22741 aagttgccgc cgacccaaat acttcaccag ccagacgaga aattacgcca gtttttgcca
    22801 tcgacatcgt gatgattgga cgatcggcat actgctcctg catctccagg gtcgcggcaa
    22861 gcaacgtcag cacatcgctg gtactttgcg gcatcagcgc aatcttagga atatcggcgt
    22921 cgaaggactg cattttgcgc agacgggcaa tgatttcttc ggcttccggc gttttatgga
    22981 agtcatggtt agacatgaca actttcacat catgcgcgtg ggcgtaggcg acggtttctt
    23041 taacctgatc atcgccggta aataactcca gatcgatcat atcaaccagg ccactgtcga
    23101 tggctgcacg attgagtgca atataagcct cggtggaaat cgcctgctcg ccgccttctt
    23161 tggcactgcg gaaggtaaac agcagcggtt tttctggcat ggtttcacgg agaatttttg
    23221 ctgccgccat gacagactcc acattggaga ggtcggcata gtggtcaaca cgccattcca
    23281 gaatatcaaa gtccgcttca cgataggcga gagcttcgga tttcacgctg gcgatatctt
    23341 tcgccatcag cgagacgatg attttaggtg cgcccgtacc aatgacgaga tcttttacag
    23401 ttacggtttt cattttttac cctttctgca cgcggtcagc ctgtcaggca ccgaacccca
    23461 tgacctgttt aacatattcc agagggaaat ctttgccagt ccataacgtg aactgttcag
    23521 ccccttgcca caacaacatg ccgtatccat caatcgtttt gcaacccgct tgttgcgcct
    23581 gctgcaataa cttcgtcata tgcgggttat acacgcattc agtgaccaga agtcccggat
    23641 gtaacagact gatatcatga accaatgatt cattctcaag gggtttcata cccacttttg
    23701 tgccattggt taaaatgtcg gcggaagcca gggcttcagc aaaggcttgc tgatcggcga
    23761 gatcggtaac cgtgacgaca caatcggtgt tttcgttaac ccgctgcgcg aaggcgaggg
    23821 ctttatcgaa gaactcatcc cgacggttaa agagtttaat ttcttttaaa ccttcaattg
    23881 ccccctgcgc gccaattgcc gttgaggcac caccggcccc taatagcacc atcgttttgc
    23941 ctttgatatc aaaaccgctc tctttaatgg cgcgaatatg acccgtgccg tcggtgttat
    24001 agccacgcag atagccatca tcattaacga tggtgttgat ggcacccacc agtttggccg
    24061 ctggcgttaa ttcatcaaca tattcacacg ccagttgttt gttcggcatc gacacaccag
    24121 ttccacgcat tttgagggct tttaatcctt caattgctgc gggaaaacta tcgttatcca
    24181 cttcaaaggc catataggta aatggcaatc ccgctttttc taaggcttta ttctgcattt
    24241 cgggcgataa actgtggcgg atgggatagg ccatcaaccc aatcaattcg tattttgcgg
    24301 taacattcat aattaactcc tttaacctct atgcttaatt gtgtttaacc gggtgctaaa
    24361 tttatgctcg ccaaaccgca catcattttc aggaataatg aaaacgcggt aatagcggat
    24421 aaaaacaata attgcggtaa tcagggccag cagcgcgaaa gtaaaatcga gaacaatgat
    24481 atattgcagg ccgatgttcg acagataacc ggtaatcagc ggaataacaa agttagccag
    24541 tccacccatc atcatatata tactggtgac tttggctttg ctttttggga aaaactctga
    24601 catgaccgaa acgccgagct gtaaaatgcc gccagctgct gagaaaccga taacaaaggc
    24661 tccggcatta cacaccaatg gagaagggta caggtagata atggcagctg ttattgttgc
    24721 cagtgcagag ttaaatgcat tagcccagat gggccggacc atttttttca gcagtgcggc
    24781 aaaaataaag acacagacca gcgaacccat actgtaataa gagatggttt ttaatgcctc
    24841 agcttctgac ataccagcaa aagccatcgc atatttgggc atccacacca caatcacata
    24901 aaaggtcgag aatgcggcta caccgaacag tactgatgaa acaccttcca gccagactaa
    24961 cggtttgctg ttcatttgcg gtaattcatt agctacgctg gcgtccacca actggctggg
    25021 gaatttgctt ttcaacagca tcagcgtgat cagtacaaac agaatacccg gaataatcag
    25081 cccatagccg taccagatgt tattgagcaa catatagctc accagcattg ggtagaacat
    25141 ttgcccgaat gacaccatcg ctttaaccag tatgaccgcc gaaccagagg ctttcggaaa
    25201 gcattccatg agcgcggggt agccacccgt atccagcgct gagttagcga tacctacgca
    25261 cactgccaga cagtaggcga gagttaaatt cgggcaagca ggaataccaa agaagaatag
    25321 cagatacatt attactgcca ttaatatcac cgcccgacga ccaaacttat cggagatcac
    25381 accgaagaat aaaatactga tcaatcgccc caaaccgata ccggaaatta aataggcaat
    25441 gcccgcgttg tcagtggaaa acttttctgc cagagatgtc atgttttggg caagcgtaat
    25501 aacactaatg ccgtgcagga aatagctgaa gtaaatacaa agaacagcca ggataaatgg
    25561 cgtgctgaaa gccttatttt gagacatttt cacagctcct tgctgaaaga taatttcgtt
    25621 accgtacgta ttgttctccg acggtttaca taattactat actgaagatg attttccctg
    25681 tgtacatggc ataaagttta tgtttccagg aatattgcat gtcatagttt gtttttgatc
    25741 gcgattttcc ttagttagcg cagaaagagt tatttatccg taagatcttg cataacctga
    25801 aaagaatatt gcattaccca cccggagcga cattttcctt tagcgagtga tgccgcgttt
    25861 ctttgcgact gcgtaagcca ataaacagtg ccagtataaa gccgatggca gcgatggcgg
    25921 tatcgaacca cataatatcg gcaatacttc tttgcgaaag atgagccgta atcaacggaa
    25981 tggtaaatgt tgcaatgctg cccgcactgt aatagatccc cgttgctttt cctttagcgt
    26041 agggaaaacg ttccgccatt aacgtcaggc caatttgtac aacaccgcct gcggaggtaa
    26101 aaccgatgac gaaagcaaat attatcacca cataaaatgt gggatgcagg cagacggtga
    26161 gcagggcgat aaatgagata aaggtgtaca gcatcagtaa tgttgttggg cgaacggtat
    26221 tacgaatgag tggagcggta ataaatacac aaagcagcga acccacggtg tagatactga
    26281 gtagtttgat cgacatagtg tatgacatgc ctgcaacaaa ttgtccgtat tgtgccagcc
    26341 actggctaac cagataaaac gttgccattg agatatagcc atataaggta taactggcta
    26401 aatcgataat tgaacagcga tgttccgtag agctggtggt tttctttatg acaggtaagc
    26461 gacgacccgg atggggtggg aacgtacaac ggtataaaaa cagagcgtta ataaacataa
    26521 tgcctgcggc aatcataaag gaccaaccga accacagttc agcccacact aacaggctga
    26581 taattagcgg taataaaaat tgtccgctgg aaacaaatgc tttaattaaa atattggctg
    26641 tacccggtga gcgtggaaaa gcttccatca aactgggata ggtgcctgca tcgagaaaac
    26701 tgtttgccat tcccgccaga aagccaaaaa cataagcgat aatgatgtta ttggtgtgca
    26761 ggatgccaaa aaagaaggcc atatagcagc acatcccgag catgataaaa gggcggcgac
    26821 caaagcgatc ggataataat cctgcaaaaa gcaagacact taatcgacca atgcccagtg
    26881 atgagataac tatcgagacg cccgcggcat tagtctgcca aagcgtctcc agcgaggcca
    26941 tattcaggct catcaaaatg acgcccatac catgcaccag ataattaaaa tacaacccca
    27001 gtgcggtagg gtaataggga tttttcatag gttctactta gcaaaggtgc tttaaaaagt
    27061 gtagcgatgt tgtgtcagta tccgttcatt attgttgtaa caacgacaat tcctttctgt
    27121 ggggaaatta acaaagccgc tccagatcgt gtgcatataa ttcagcggct accgattgat
    27181 agatcttcca gtcaataaaa ttgccatcag ggtagacttg ttgaaacgcg gttaagtcgg
    27241 ggaaaaaacg catggtgttg ttgccgatgc ggttatttat tttctctatg atggcatgaa
    27301 gatgtttttt tcgttgctga cgcatagcca acagttgctc gactacacaa tcaggaatgg
    27361 cgcatttgcc attttcccac tgacgccagc tttcactgtc accggtctgg gcaatccagg
    27421 tcgcgcattc atcaatagtc atggcaaaaa tatggcgaag tgcctggagt tcataagcgt
    27481 tcatcgtgta atggctgcat gaaataaaca ataaaatata aataacatta tcagcctgct
    27541 gacggcttga agagagtcac agtatcttgt gcaacattat tggaaagcgt gaggggcgag
    27601 gtggcgaagc ggaaaaatgt taaaaaaaag cccatcgtgg gagatgggca aagactacac
    27661 acagcaattc gttgtttcac tcaggggatt tccatgctta taaatcaata tgttgattta
    27721 taaccgtgag ctaatagtag gcatacggac tttttgcgtc gatcagattc gtctcattag
    27781 tttaaaaact gtaaagaatt tgcgacaggt actaccagct gaatggcggt aagcaggaaa
    27841 atatggggaa gagcaagcaa atatcaatta gcaggggtta acaatcaggc ggcagagccg
    27901 cctgatgaga aattacttat tcgatttctc gatttcgcca agttcttcga gaatttcttc
    27961 cggttggtcc gtcggcggtg ggacttcttc cacccacgcg gcaaaaagac gccaggaaac
    28021 ggctaacaga accggaccaa taaacaggcc gatcatcccg aaagcaatca aaccaccaat
    28081 aacgccagag agaatcagga tcagcggtaa atcggcaccc atgcgaatta acattgggcg
    28141 gatgacgtta tccagcgtgc caaccacacc gctccacact aacaataccg ttccccaggt
    28201 ggtatcgcca gtccagtaga gccagataat cgccggaatc agtaccggca acggtccaag
    28261 ctggacaagg caggagagga tcattaacac cgtgagcaaa gttgcataag gtacgccgga
    28321 tacggcgagg ccgataccgc caagcactgc ctgtactaac gctgtcacca caacacccag
    28381 cgccaccgcg cggatagcct gtgccgccag cagcacggcg gcatctccac gaacgcctgc
    28441 cagacgggtt gcaaaatggc gaatgccttg tgccacctgt tcaccgcgcc agtacagcag
    28501 ggcgctgaag agaagcatca gcgcacaatg caccataaag cgcccgatat gcgccgcctg
    28561 cccaacgaac caggtggtgg tggtgccaat ataaggacgg acttttgcca tgatcgccgt
    28621 cccccccata tccagcaagt tgtgccagcc tgcatatagc ttcgcgccaa tcaccggaat
    28681 ggtattaagc cacgccagat cgggtaacgt catgtcaccg ctggaaatgg ctttaattag
    28741 cgggccgctg ccgtcgacga tactgttaac cagcaaggca atagggatga taaacaccat
    28801 cactaataac agcgtcatca ccagaacggc gagagagcgg cgaccaaaca tgaacttttg
    28861 caaacgtaac aataccggcc aggtggcgat aaccaccgta ccagcccatg caaagccgag
    28921 aataaagggt tgtacaatcc acagacatgc cacaatcatg atggctaaaa agagcaccga
    28981 aagcagaatt tgtgcgacat ccctgggctg acgaacattt accataacta cttttcacct
    29041 tttttgtgcg tcaaatcgtc gacgcgacgt gaacacgcga aactcacctg cataatgatg
    29101 agcaatttca gcggttttta acaggccgat tctgccctta attctgatgg gcgtgttaga
    29161 aaaaaatgtg gtacaacaac tcaggcaaca cgcaaacgat ttactcgtcg tatttcaaat
    29221 tgcatgtgct gcggctacgt tcgttcaccc cagtcactta ctattgtaag ctcctggagt
    29281 tcactcgctt gccgccttcc tgcaacacga aatccataga gtaaacaaac atcacctttc
    29341 agtgcgctat tgcagcagcc tgaaagaaca gtgtatcgac ctacatcaca gaccgcagga
    29401 aagggtcaat ataatgattc cacagatttc ccaggcacct ggcgtcgttc aattggtgct
    29461 taattttttg caagagctgg agcaacaggg ttttactggc gatacggcga caagttatgc
    29521 cgatcgtctg acaatgtcga ccgacaacag tatttaccaa cttctccccg atgcggtggt
    29581 atttccgcgt tcaaccgcag atgtagcgct gatcgcccgt cttgccgcgc aggaacgcta
    29641 ttcatcgttg atctttaccc cccgcggcgg cggcaccggc actaacggtc aggcgctcaa
    29701 ccaggggatt attgttgata tgtcccgcca tatgaaccgc atcatcgaaa ttaaccctgc
    29761 agagggctgg gtgcgcgttg aggccggggt gataaaagac caactcaatc agtacctgaa
    29821 accgttcggc tacttttttg cgccggagct ttcgaccagc aaccgggcaa cgctcggcgg
    29881 gatgatcaat accgatgcat ccggtcaggg atcgctggtc tatggcaaaa cgtcagatca
    29941 cgtacttggc gtacgcgcgg tgttgttggg gggcgatatt ctcgatacgc aacctttacc
    30001 cgtcgaactg gcggaaacgc tgggtaaatc caataccaca atcgggcgaa tttataacac
    30061 ggtttatcaa cgttgccgtc agcaacgcca gttaatcatc aacaacttcc ccaaacttaa
    30121 ccgctttctt accggttacg atctgcgtca tgtctttaac gatgagatga ccgagttcga
    30181 tctgacgcgc attctgacgg gttcagaagg gacgctggcc tttattaccg aagcgcggct
    30241 ggatattacg cgcttgccta aagtgcggcg tctggtgaac gtcaaatatg actcttttga
    30301 ctccgcgctg cgtaacgcgc cgtttatggt tgaggcgcgg gcgctttcgg tagagacggt
    30361 ggactcaaaa gtgctgaatc tggcgcggga agatattgtc tggcattccg tcagcgagtt
    30421 gattaccgat gtacctgaca aagaaatgct cgggctgaac attgtggaat ttgctggtga
    30481 tgatgaggcg ctgattgacg agcgagttaa tgcactctgt gtgcggcttg atgagctgat
    30541 tgccagccaa caggcaggtg tgattggctg gcaggtgtgc cgcgaactgg cgggcgttga
    30601 acgtatctat gcgatgcgca aaaaagccgt tggtctgctt ggcaatgcca aaggtgccgc
    30661 taagccaatt ccgtttgctg aggatacctg cgtaccgccg gaacatctgg ccgattatat
    30721 tgctgaattt cgcgcgctgc tcgacagcca cggcttaagc tacggtatgt tcggtcacgt
    30781 cgacgcaggt gtcttgcacg tccgtccggc actggatatg tgcgatccac aacaagagat
    30841 tttgatgaag caaatctctg atgacgtggt ggcgctgact gcgaaatacg gtggtttgtt
    30901 gtggggcgag cacggcaaag gttttcgcgc tgaatacagc ccggcgtttt tcggtgagga
    30961 actttttgca gaactgcgca aagtgaaagc ggcatttgac ccgcataacc gactcaaccc
    31021 agggaagatt tgcccgccag aaggtctcga tgcgccgatg atgaaagtgg acgcggtgaa
    31081 gcgcggtaca tttgatcggc agatccccat tgcggtacgc cagcagtggc gcggtgcgat
    31141 ggagtgtaac ggcaacggtt tatgcttcaa ctttgatgcc cgtagtccga tgtgtccgtc
    31201 gatgaagatc acccagaacc ggattcattc accgaaaggg cgcgcaacgc tggtgcgtga
    31261 atggctgcgc ttgttggcgg atcgcggcgt tgacccactc aaactggaac aagaactgcc
    31321 tgaatccggc gtcagtttac ggacgttaat tgcccgcacg cgcaatagct ggcatgcgaa
    31381 taaaggcgaa tacgacttct cacacgaagt caaagaggct atgtcgggct gtctggcctg
    31441 taaagcgtgt tcgacccagt gccccatcaa aattgatgtg ccggagtttc gttctcgttt
    31501 tctgcagctc tatcacaccc gttatttacg cccgcttcgt gaccacctcg ttgctacggt
    31561 cgagagctac gcaccgctga tggcacgcgc gccgaaaacc tttaacttct tcattaacca
    31621 gccgctggtg cgcaaactct cggaaaaaca tatcggcatg gttgatttgc cactgctgtc
    31681 agtcccctcg ctacaacaac aaatggtggg gcatcgctcg gcgaatatga cgctggaaca
    31741 gcttgaagct ctcaatacag agcagaaagc gcgcacggtg ttggtggtgc aggacccctt
    31801 taccagctat tacgatgcgc aagtggtggc ggattttgtc cgtctggttg aaaaattagg
    31861 tttccagcct gtgttactgc cattttcgcc aaatggcaaa gcccagcata tcaaaggctt
    31921 tcttaatcgt tttgcgaaga cggcgaaaaa gacggcggat ttcctcaacc gtatggcgaa
    31981 gctggggatg ccaatggtgg gcgtcgatcc ggcgctggta ctttgttatc gcgatgaata
    32041 taaactggcg ctgggcgatg aacgtggcga gtttaacgtc ttactggcga atgaatggct
    32101 ggcaagcgca cttgactcac agccagtggc tacagtcagc ggtgaatcat ggtatttctt
    32161 tggtcactgt accgaagtta ccgctttgcc gggcgcgcca gcacaatggg ccgcgatatt
    32221 tgcccgtttt ggcgcgaaac tggaaaatgt cagcgtgggt tgctgcggca tggcagggac
    32281 ttacggacat gaagcgaaaa accataaaaa ttcgctcggg atctatgagt tatcctggca
    32341 tcaggcgatg cagcgactgc cgcgtaatcg ctgtctggcg accggatatt cctgccgtag
    32401 ccaggtaaaa cgggtcgaag gcacgggggt acgccatcct gtgcaggctt tactggagat
    32461 tattaaatga tatggaaacg aaaaatcacc ctggaagcat tgaatgctat gggtgaagga
    32521 aacatggtgg ggttgctgga tattcgcttt gaacatattg gtgatgacac ccttgaagcg
    32581 acaatgccag tagactcccg gacaaagcag cctttcgggt tgctgcatgg aggcgcgtct
    32641 gtggtgctgg ccgaaagtat cggttccgtt gccggttatt tatgtaccga aggtgagcaa
    32701 aaggtggttg gtctggaaat caatgctaac cacgtccgct cggcacgaga agggcgggtg
    32761 cgcggcgtat gcaaaccgtt gcatctcggt tcgcgtcacc aggtctggca gattgaaatc
    32821 ttcgatgaga aagggcgttt gtgctgttcg tcacgattga cgaccgccat tttgtgacgt
    32881 tgtcaccaga aaagtgtgac gcatattgct tttggtcatt atctgcacag taaacggtgg
    32941 tatactgtgc aggtcttgtc taaaagcgag gatgctatgt ctactcaact tgatcccacc
    33001 cagctggcca ttgaattttt acgccgtgac cagagcaatc tttcacctgc ccaatatctc
    33061 aaacgtctga aacaactaga acttgaattt gctgacctgc ttactctctc ttctgctgaa
    33121 ttaaaagaag aaatctactt tgcctggcgt ctgggcgtgc attgatcctg acttaatgcc
    33181 gcaagttcgc catatcgacc tggattaatc tccgtccagg ttcgatggcc ttcttaatac
    33241 tgcttatcta cccgcctgat attgttcaaa aataatacta atcgcagttt gtgttaaaac
    33301 ggcgggttag ctttatgaga ttattcttat cgccccttca agagctaagc cactgagagt
    33361 gccggagata agcgccggat ggggtagaaa cccttaagcc tgtgtcgcac agacttaagg
    33421 gttttcttat ttctgaacgc tgcacttttt tacctatctc ttaaatagtc attttcaata
    33481 caactttttc tttttcatca cagccttaat gctccttaga taataatcat tatctaacaa
    33541 tgagatacct aattcttagc gtgcctgtta acccacacat cagggtctat gcttattaaa
    33601 agcaggaaaa aagcatgttt tacggtaaag cccctgcgtt tgctgggttg aactgataat
    33661 cattatcact aacatgctgt tatacgctga aagcgatgaa gtgaggtaaa tcgatggaca
    33721 tgcattcagg aacctttaat ccacaggatt tcgcctggca gggcttaacg ctgacacccg
    33781 cagccgctgt ccacatccgc gagctggtgg caaagcagcc gggtatggtc ggcgtgcgct
    33841 taggcgtgaa gcaaacgggc tgcgcgggct ttggctatgt gctcgacagt gttagcgagc
    33901 cggacaaaga cgatctgctg tttgaacacg acggtgcgaa gctgtttgtc ccgctgcaag
    33961 cgatgccgtt tattgatggc acggaagtcg atttcgttcg tgaaggactt aatcagatat
    34021 tcaaatttca caaccctaaa gcccagaatg aatgtggctg tggcgaaagc tttggggtat
    34081 aggcggtact atgtctcgta atactgaagc aactgacgat gtcaaaacct ggaccggcgg
    34141 cccgctgaat tataaagaag gattcttcac ccagttagcc accgatgagc tggcaaaggg
    34201 gataaacgaa gaggtggtgc gcgcaatttc ggcgaagcgt aatgagccgg agtggatgct
    34261 ggagtttcgt ctcaacgcct atcgcgcatg gctggagatg gaagaaccgc actggttgaa
    34321 agcgcactac gacaagctga attatcagga ttacagctac tactcagcac catcgtgcgg
    34381 taattgtgac gacaactgcg cgtctgaacc cggcgcggtg cagcaaactg gtgcgaacgc
    34441 ctttttaagt aaagaggtgg aggcggcgtt tgagcagttg ggcgttcccg tgcgggaagg
    34501 caaagaggtg gcggtggatg ccattttcga ctctgtttcg gttgccacca cttatcgtga
    34561 aaaactggcg gagcagggaa ttattttctg ttcctttggc gaggcgatcc acgatcaccc
    34621 ggaacttgtg cgtaaatatc tcggcaccgt ggtgccgggg aatgacaact tctttgccgc
    34681 acttaatgcg gcggtagcct ctgacggaac gtttatttat gtgcctaaag gcgtgcgctg
    34741 cccgatggaa ctttccacct attttcgcat taacgcggaa aaaacagggc agtttgagcg
    34801 caccattctg gtggccgacg aagacagcta tgtcagctac attgaaggct gttccgctcc
    34861 ggtgcgtgac agctatcagt tacacgcggc ggtggttgaa gtcatcatcc ataaaaacgc
    34921 cgaggtgaaa tattccacgg tacaaaactg gttccctggc gataacaaca ccggcggtat
    34981 tctcaacttc gtcaccaagc gtgctttgtg cgaaggcgaa aacagcaaaa tgtcatggac
    35041 gcaatcagaa accgggtcag cgattacgtg gaaatatccc agttgcattt tgcgcggcga
    35101 taactccatt ggtgagtttt actcagtggc actgaccagc ggtcatcagc aagcggatac
    35161 cggcaccaag atgatccaca tcggtaaaaa caccaaatcg accattatct cgaaaggggt
    35221 ctctgccgga catagtcaga acagttatcg cggcttagtg aaaatcatgc cgacggcaac
    35281 caatgcgcgc aatttcactc agtgcgactc aatgctgatt ggcgctaatt gtggggcgca
    35341 taccttcccg tatgtcgaat gtcgcaataa cagcgcacaa ctggagcacg aggcaacgac
    35401 atcacgtatt ggtgaagatc aactgtttta ctgcctgcaa cgcgggatca gcgaagaaga
    35461 cgccatctcg atgattgtta acggtttctg caaagacgtg ttctcagaac tgccgctgga
    35521 atttgccgtt gaagcacaaa aactcctcgc catcagtctt gaacacagcg tcggataagg
    35581 aataaacatg ttaagtatta aagatttaca cgtcagcgtg gaagataaag ctatcctgcg
    35641 tggattaa
//
LOCUS       DAMZFT010000039        34143 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.039, whole genome shotgun sequence.
ACCESSION   DAMZFT010000039 DAMZFT010000000
VERSION     DAMZFT010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 34143)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34143)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34143
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.039"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(200..275)
                     /locus_tag="PN977_001992"
     tRNA            complement(200..275)
                     /locus_tag="PN977_001992"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(240..242),aa:Ile,seq:cat)
     gene            832..3084
                     /locus_tag="PN977_001993"
     CDS             832..3084
                     /locus_tag="PN977_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6042022.1"
                     /translation="MFSIKPGPRNLPIDNPTLLSWNITDGDLNSKLNTLEYLNCITNI
                     INACGVYPQDLKDREIISTFHAEKVINDLLKNDYKISLSPDTTYRELNKAAQRSITAP
                     DRIGEGKTWVYQRDTMVERGDNSSVHQYGPAEHFTHIISDKPSPKDKYVAYAINIPDY
                     ELAADVYNINVTSPSGQQETFKILINPEHLRQTLERKSLTAVQKSQCEIITPKKPGEA
                     ILHAFNATYQQIRENMSEFARSHYGYIQIPPVTTFRADGPETPEEEKGYWFHAYQPED
                     LCTIHNPMGDLQDFIALVKDAKKFGIDIIPDYTFNFMGIGGSGKNDLDYPSADIRAKI
                     SKDIESGIPGYWQGQVLIPFTIDPVTKERKQIHPEDIHLTAKDFETSKDNISKDEWEN
                     LHALKEKRLNGMPKTTPKSDQVIMLQNQYVREMRKYGVRGLRYDAAKHSKHEQIERSI
                     TPPLTIYNERLHNTNLFKPIYHEKAVMNYMEYLVTCQLDEEQMSSLLYERDDLSAIDF
                     SLLMKTIKAFSFGGDLQTLASKPGSTISSIPSKRRILININHDFPNNGNLFNDFLFNH
                     QQDEQLAMAYMAALPFSRPLVYWDGQVLKSTTEIKNYDGSTRVGGEAWLNKGCSTYQQ
                     LYNEFHALYIDKAGIWSAFEGVFATKNVLAFSRGDSVNINHSPHDGLVIINKGNEEVE
                     GTWPNKLQPGIYKNMGSNSVNIIINNTRKIIPPGKAFMLRGGTLNINIPGRSALLLGK
                     TGEPPNYLYL"
     gene            3421..4398
                     /gene="csiD"
                     /locus_tag="PN977_001994"
     CDS             3421..4398
                     /gene="csiD"
                     /locus_tag="PN977_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311547.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation induced protein CsiD"
                     /protein_id="HDI6042023.1"
                     /translation="MNALTAVQNNAVDSGQDYSGFTLIPSAQSPRLLELTFTEQTTKQ
                     FLEQVAEWPVQALEYKSFLRFRVGKILDDLCANQLQPLLLKTLLNRAEGALLINAVGV
                     DDVKQADEMVKLATAVAHLIGRSNFDAMSGQYYARFVVKNVDNSDSYLRQPHRVMELH
                     NDGTYVEEITDYVLMMKIDEQNMQGGNSLLLHLDDWEHLDHYFRHPLARRPMRFAAPP
                     SKNVSKDVFHPVFDVDQQGRPVMRYIDQFVQPKDFEEGVWLSELSDAIETSKGILSVP
                     VPVGKFLLINNLFWLHGRDRFTPHPDLRRELMRQRGYFAYATNHYQTHQ"
     gene            4418..5686
                     /gene="lhgO"
                     /locus_tag="PN977_001995"
     CDS             4418..5686
                     /gene="lhgO"
                     /locus_tag="PN977_001995"
                     /EC_number="1.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-hydroxyglutarate oxidase"
                     /protein_id="HDI6042024.1"
                     /translation="MYDFVIIGGGIIGMSTAMQLIDVYPDARIALLEKESGPAFHQTG
                     HNSGVIHAGVYYTPGSLKAQFCLAGNRATKAFCDQNGIRYDNCGKMLVATSELEMERM
                     RALWERTAANGIEREWLNAEELREREPNITGLGGIFVPSSGIVSYREVTAAMAKIFQA
                     RGGEIIYNAEVSALSEHKNGVVIRTRQGGEYEASTLISCSGLMADRLVKMLGLEPGFI
                     ICPFRGEYFRLAPEHNQIVNHLIYPIPDPAMPFLGVHLTRMIDGSVTVGPNAVLAFKR
                     EGYRKRDFSFSDTLEILGSSGIRRVLQNHLRSGLGEMKNSLCKSGYLRLVQKYCPRLS
                     LSDLQPWPAGVRAQAVSPDGKLIDDFLFVTTPRTIHTCNAPSPAATSAIPIGAHIVSK
                     VQTLLASQSNPGRTLRAARSVDALHAAFNQ"
     gene            5709..7157
                     /gene="gabD"
                     /locus_tag="PN977_001996"
     CDS             5709..7157
                     /gene="gabD"
                     /locus_tag="PN977_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HDI6042025.1"
                     /translation="MKLNDSNLFRQQALINGEWLDANNGEVIDVTNPANGDKLGSVPK
                     MGADETRAAIDAANRALPAWRALTAKERANILRNWFNLMMEHQDDLARLMTLEQGKPL
                     AEAKGEISYAASFIEWFAEEGKRIYGDTIPGHQADKRLIVIKQPIGVTAAITPWNFPA
                     AMITRKAGPALAAGCTMVLKPASQTPFSALALAELAIRAGVPAGVFNVVTGSAGAVGN
                     ELTSNPLVRKLSFTGSTEIGRQLMEQCAKDIKKVSLELGGNAPFIVFDDADLDKAVEG
                     ALASKFRNAGQTCVCANRLYVQNGVYDRFAEKLQQAVSKLHIGNGLEKGVTIGPLIDE
                     KAVAKVEEHIADALEKGARVVCGGKAHERGGNFFQPTILVDVPANAKVSKEETFGPLA
                     PLFRFKDEADVIAQANDTEFGLAAYFYARDLSRVFRVGEALEYGIVGINTGIISNEVA
                     PFGGIKASGLGREGSKYGIEDYLEIKYMCIGL"
     gene            7171..8451
                     /gene="gabT"
                     /locus_tag="PN977_001997"
     CDS             7171..8451
                     /gene="gabT"
                     /locus_tag="PN977_001997"
                     /EC_number="2.6.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000097653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="HDI6042026.1"
                     /translation="MSSNKELMQRRSQAIPRGVGQIHPIFADRAENCRVWDVEGREYL
                     DFAGGIAVLNTGHLHPKVVAAVEAQLKKLSHTCFQVLAYEPYLELCEIMNQKVPGDFA
                     KKTLLVTTGSEAVENAVKIARAATKRSGTIAFSGAYHGRTHYTLALTGKVNPYSAGMG
                     LMPGHVYRALYPCPLHGISEDDAIASIHRIFKNDAAPEDIAAIVIEPVQGEGGFYAAT
                     PAFMQRLRALCDEHGIMLIADEVQSGAGRTGTLFAMEQMGVAPDLTTFAKSIAGGFPL
                     AGVTGRAEVMDAVAPGGLGGTYAGNPIACVAALEVLKVFEQENLLQKANVLGQKLKDG
                     LLAIAEKHPEIGDVRGLGAMIAIELFEDGDPSKPDAKLTAEIVARARDKGLILLSCGP
                     YYNVLRILVPLTIEDAQIRQGLEIISQCFAEAKL"
     gene            8688..10088
                     /gene="gabP"
                     /locus_tag="PN977_001998"
     CDS             8688..10088
                     /gene="gabP"
                     /locus_tag="PN977_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GABA permease"
                     /protein_id="HDI6042027.1"
                     /translation="MGQSSQPHELGGGLKSRHVTMLSIAGVIGASLFVGSSVAIAEAG
                     PAVLLAYLFAGLLVVMIMRMLAEMAVATPDTGSFSTYADKAIGRWAGYTIGWLYWWFW
                     VLVIPLEANIAAMILHSWVPGIPIWLFSLVITLALTGSNLLSVKNYGEFEFWLALCKV
                     IAILAFIFLGAVAISGFYPYAEVSGISRLWDSGGFMPNGFGAVLSAMLITMFSFMGAE
                     IVTIAAAESDTPEKHIVRATNSVIWRISIFYLCSIFVVVALIPWNMPGLKAVGSYRSV
                     LELLNIPHAKLIMDCVILLSVTSCLNSALYTASRMLYSLSRRGDAPAVMGKINRSKTP
                     YVAVLLSTGAAFLTVVVNYYAPAKVFKFLIDSSGAIALLVYLVIAVSQLRMRKILRAE
                     GSEIRLRMWLYPWLTWLVIGFITFVLVVMLFRPAQQLEVISTGLLAIGIICTVPIMAR
                     WKKLVLWQKTPVHNTR"
     gene            10109..10771
                     /gene="csiR"
                     /locus_tag="PN977_001999"
     CDS             10109..10771
                     /gene="csiR"
                     /locus_tag="PN977_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311552.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CsiR"
                     /protein_id="HDI6042028.1"
                     /translation="MTITSLDGYRWLKNDIIRGNFQPDEKLRMSLLTSRYALGVGPLR
                     EALSQLVAERLVTVVNQKGYRVASMSEQELLDIFDARANMEAMLVSLAIARGGDEWEA
                     DVLAKAHLLSKLEACDASEKMLDEWDLRHQAFHTAIVAGCGSHYLLQMRERLFDLAAR
                     YRFIWLRRTVLSVEMLEDKHDQHQTLTAAVLARDTARASELMRQHLLTPIPIIQQAMA
                     GN"
     gene            complement(10772..11221)
                     /gene="kbp"
                     /locus_tag="PN977_002000"
     CDS             complement(10772..11221)
                     /gene="kbp"
                     /locus_tag="PN977_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium binding protein Kbp"
                     /protein_id="HDI6042029.1"
                     /translation="MGLFNFVKDAGEKLWDAVTGQHDKDDQAKKVQEHLNKTGIPDAD
                     KVNIQIADGKATVTGDGLSQEAKEKILVAVGNISGIASVDDQVKTATPAAASQFYTVK
                     SGDTLSAISKQVYGNANLYNKIFEANKPMLKSPDKIYPGQVLRIPEE"
     gene            complement(11305..11463)
                     /locus_tag="PN977_002001"
     CDS             complement(11305..11463)
                     /locus_tag="PN977_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqaE/Pmp3 family membrane protein"
                     /protein_id="HDI6042030.1"
                     /translation="MGFWRIVITIILPPLGVLLGKGFGWAFIINILLTLLGYIPGLIH
                     AFWVQTRD"
     gene            11646..11945
                     /locus_tag="PN977_002002"
     CDS             11646..11945
                     /locus_tag="PN977_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDI6042031.1"
                     /translation="MTELAQLQASAEQAAALLKAMSHPKRLLILCMLSGSPGTSAGEL
                     TRITGLSASATSQHLARMRDEGLIDRQRDAQRILYSIKNEAVNAIIATLKNVYCP"
     gene            11955..12479
                     /gene="ygaP"
                     /locus_tag="PN977_002003"
     CDS             11955..12479
                     /gene="ygaP"
                     /locus_tag="PN977_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YgaP"
                     /protein_id="HDI6042032.1"
                     /translation="MALTTISPHDAQELIARGAKLIDIRDADEYLREHIPEADLAPLS
                     VLEQSGLPAKLRRDQIIFHCQAGKRTSNNADKLAAIAAPAEIFLLEDGIDGWKKAGLL
                     VAVNKSQPLPLMRQVQIAAGGLILIGVILGYTVNSGFFLLSGFVGAGLLFAGISGFCG
                     MARLLDKMPWNQRA"
     gene            complement(12526..12930)
                     /gene="stpA"
                     /locus_tag="PN977_002004"
     CDS             complement(12526..12930)
                     /gene="stpA"
                     /locus_tag="PN977_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein StpA"
                     /protein_id="HDI6042033.1"
                     /translation="MSVMLQSLNNIRTLRAMAREFSIDVLEEMLEKFRVVTKERREEE
                     EQQQRELAERQEKISTWLELMKADGINPEELLGNSSAAAPRAGKKRQPRPAKYKFTDV
                     NGETKTWTGQGRTPKPIAQALAEGKSLDDFLI"
     gene            13598..14047
                     /gene="alaE"
                     /locus_tag="PN977_002005"
     CDS             13598..14047
                     /gene="alaE"
                     /locus_tag="PN977_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-alanine exporter AlaE"
                     /protein_id="HDI6042034.1"
                     /translation="MFSPQSRLRHAVADTFAMVVYCSVVNMCIEVFLSGMSFEQSFYS
                     RLVAIPVNILIAWPYGMYRDLFMRAARKVSPSGWIKNLADILAYVTFQSPVYVAILLV
                     VGADWHQIMAAVSSNIVVSMLMGAVYGYFLDYCRRLFKVSRYQQVKA"
     gene            complement(14084..14428)
                     /locus_tag="PN977_002006"
     CDS             complement(14084..14428)
                     /locus_tag="PN977_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgaC family protein"
                     /protein_id="HDI6042035.1"
                     /translation="MYLRPDEVARVLEKVGFTVDVVTQKAYGYRRGENYVYVNREARM
                     GRTALVIHPTLKERSSTLAEPASDIKTCDHYQQFPLYLAGERHEHYGIPHGFSSRVAL
                     ERYLNGLFGEAS"
     gene            14580..14909
                     /locus_tag="PN977_002007"
     CDS             14580..14909
                     /locus_tag="PN977_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 domain-containing protein"
                     /protein_id="HDI6042036.1"
                     /translation="MFNRPNRNDVDDGVQDIQNDVNQLADSLESVLKSWCSDAKGEAE
                     AARSKAQALLKETRARMHGRTRVQQAARDAVGCADSFVRERPWCSVGTAAAVGIFIGA
                     LLSMRKS"
     gene            complement(14966..16300)
                     /locus_tag="PN977_002008"
     CDS             complement(14966..16300)
                     /locus_tag="PN977_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HDI6042037.1"
                     /translation="MPRYQDIARQLKTAIEQGELKPGARLPSSRTWSQELGVSRSTVE
                     NAYAELVAQGWLIRRGQAGTFVSERIYPQQSTVQVVAFAGESQQPLPFQMGLPALDLF
                     PRELWARVMGRRLRTQTRFDLALGDVCGEAALREAIVDYLRVSRGIDCQPEQVFITHG
                     YAASIALILHALAKPGNGMWIEDPGFPLIRPIVTRHDVEILPVPVDDNGLDITSGIQN
                     YPDARFALITPAHQSPLGVALSLARRHQILEWADRSQAWIIEDDYDSEFRYHGKPLPA
                     LKSLDAPQRVIYAGTFSKALFPALRCAWLVVPVKQIAQFRHQASLAPCAVPVLWQNTL
                     ADFLREGHFWRHLKKMRQHYAQRRQWIEQALTQQGFQVVPQKGGIQMVIRMIGDDIAH
                     ARKANAAGLAVQALSDWRIRSSGEGGLLLSFTNIVNEGMARQVAQQLRKALS"
     gene            16388..16819
                     /locus_tag="PN977_002009"
     CDS             16388..16819
                     /locus_tag="PN977_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HDI6042038.1"
                     /translation="MTTLRQPYYELSPAVYNALVQAKTALENSTLDTTLMELVYLRVS
                     QINGCAFCLEMHSKALRKSGVPQHKLDALAGWRVSHHFDERERAALAWAESVTEIART
                     HAEDEVYQPLLEHFSAAEISDLTFAIGLMNCFNRLAVSMRM"
     gene            17028..17273
                     /gene="nrdH"
                     /locus_tag="PN977_002010"
     CDS             17028..17273
                     /gene="nrdH"
                     /locus_tag="PN977_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HDI6042039.1"
                     /translation="MRITIYTRNDCVQCHATKRAMENRGFDFEMINVDRVPEAAEALR
                     AQGFRQLPVVIAGDLSWSGFRPDMINRLHPAPHAASA"
     gene            17270..17680
                     /gene="nrdI"
                     /locus_tag="PN977_002011"
     CDS             17270..17680
                     /gene="nrdI"
                     /locus_tag="PN977_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HDI6042040.1"
                     /translation="MSQLVYFSSSSENTQRFIERLGLPAVRIPLNERERIQVDEPYIL
                     IVPSYGGGGTAGAVPRQVIRFLNDEHNRALLRGVIASGNRNFGEAYGRAGDVIARKCG
                     VPWLYRFELMGTQSDIENVRKGVTEFWQRQPQNA"
     gene            17653..19797
                     /gene="nrdE"
                     /locus_tag="PN977_002012"
     CDS             17653..19797
                     /gene="nrdE"
                     /locus_tag="PN977_002012"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HDI6042041.1"
                     /translation="MATTTAERLTQETMDYHALNAMLNLYDSAGRIQFDKDRQAVDAF
                     MTTHVRPNSVAFSSQQQRLNWLVNEGYYDESVLNRYSRDFVITLFAHAHASGFRFQTF
                     LGAWKFYTSYTLKTFDGKRYLEDFADRVTMVALTLAQGDETLATQLTDEMLSGRFQPA
                     TPTFLNCGKQQRGELVSCFLLRIEDNMESIGRAVNSALQLSKRGGGVAFLLSNLREAG
                     APIKRIENQSSGVIPVMKMLEDAFSYANQLGARQGAGAVYLHAHHPDILRFLDTKREN
                     ADEKIRIKTLSLGVVIPDITFHLAKENAQMALFSPYDVERVYGKPFADVAISEHYDEL
                     VADERIRKKYLNARDFFQRLAEIQFESGYPYIMYEDTVNRANPIAGRINMSNLCSEIL
                     QVNSASDYDENLDYARTGHDISCNLGSLNIAHTMDSPDFARTVETAVRGLTAVSDMSH
                     IRSVPSIEAGNAASHAIGLGQMNLHGYLAREGIAYGSPEALDFTNLYFYTITWHALRT
                     SMLLARERGETFAGFKQSRYASGEYFSQYLQSNWQPKTAKVGELFARSGVTLPTREMW
                     AQLRDDVMCYGIYNQNLQAVPPTGSISYINHATSSIHPIVAKVEIRKEGKTGRVYYPA
                     PFMTNENLALYQDAYEIGAEKIIDTYAEATRHVDQGLSLTLFFPDTATTRDINKAQIY
                     AWRKGIKTLYYIRLRQMALEGTEIEGCVSCAL"
     gene            19807..20766
                     /gene="nrdF"
                     /locus_tag="PN977_002013"
     CDS             19807..20766
                     /gene="nrdF"
                     /locus_tag="PN977_002013"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HDI6042042.1"
                     /translation="MKLSRISAINWNKISDDKDLEVWNRLTSNFWLPEKVPLSNDIPA
                     WQTLSVVEQQLTMRVFTGLTLLDTLQNVIGAPSLMPDALTPHEEAVLSNISFMEAVHA
                     RSYSSIFSTLCQTKDVDAAYAWSEENAPLQRKAQIIQQHYRGDDPLKKKIASVFLESF
                     LFYSGFWLPMYFSSRGKLTNTADLIRLIIRDEAVHGYYIGYKYQKKLEKISLAQREEL
                     KSFAFDLLLELYDNELQYTDELYAETPWSDDVKAFLCYNANKALMNLGYEPLFPAEMA
                     EVNPAILAALSPNADENHDFFSGSGSSYVMGKAVETEDEDWNF"
     gene            21122..22324
                     /gene="proV"
                     /locus_tag="PN977_002014"
     CDS             21122..22324
                     /gene="proV"
                     /locus_tag="PN977_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein ProV"
                     /protein_id="HDI6042043.1"
                     /translation="MAIKLEIKNLYKIFGEHPQRAFKYIEQGLSKEQILEKTGLSLGV
                     KDASLAIEEGEIFVIMGLSGSGKSTMVRLLNRLIEPTRGQVLIDGVDIAKISDAELRE
                     VRRKKIAMVFQSFALMPHMTVLDNTAFGMELAGINAEGRREKALDALRQVGLENYAHS
                     YPDELSGGMRQRVGLARALAINPDILLMDEAFSALDPLIRTEMQDELVKLQAKHQRTI
                     VFISHDLDEAMRIGDRIAIMQNGEVVQVGTPDEILNNPANDYVRTFFRGVDISQVFSA
                     KDIARRTPNGLIRKTPGFGPRSALKLLQDEDREYGYVIERGNKFVGAVSIDSLKTALT
                     QQQGLDAALIDAPLAVDAQTPLSELLSHVGQAPCAVPVVDEDQQYVGIISKGMLLRAL
                     DREGVNNG"
     gene            22317..23381
                     /gene="proW"
                     /locus_tag="PN977_002015"
     CDS             22317..23381
                     /gene="proW"
                     /locus_tag="PN977_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     permease ProW"
                     /protein_id="HDI6042044.1"
                     /translation="MADQNNPWDTTPAADSAAQSADAWGTPATAPTDGGGADWLTSTP
                     APNVEHFNILDPFHKTLIPLDSWVTEGIDWVVTHFRPVFQGVRLPVDYILNGFQQLLL
                     GMPAPVAIIVFALIAWQISGVGMGVATLVSLIAIGAIGAWSQAMVTLALVLTALLFCI
                     VIGLPLGIWLARSPRAAKIIRPLLDAMQTTPAFVYLVPIVMLFGIGNVPGVVVTIIFA
                     LPPIIRLTILGINQVPADLIEASRSFGASPRQMLFKVQLPLAMPTIMAGVNQTLMLAL
                     SMVVIASMIAVGGLGQMVLRGIGRLDMGLATVGGVGIVILAIILDRLTQAVGRDSRSR
                     GNRRWYTTGPVGLLTRPFIK"
     gene            23439..24431
                     /gene="proX"
                     /locus_tag="PN977_002016"
     CDS             23439..24431
                     /gene="proX"
                     /locus_tag="PN977_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     substrate-binding protein ProX"
                     /protein_id="HDI6042045.1"
                     /translation="MRHSVLFATAFATLISTQTFAADLPGKGITVNPVQSTITEETFQ
                     TLLVSRALEKLGYTVNKPSEVDYNVGYTSLASGDATFTAVNWTPLHDNMYEAAGGDKK
                     FYREGVFVNGAAQGYLIDKKTADQYKITNIAQLKDPKIAKLFDTNGDGKADLTGCNPG
                     WGCEGAINHQLAAYGLTNTVTHNQGNYAAMMADTISRYKEGKPVFYYTWTPYWVSNEL
                     KPGKDVVWLQVPFSALPGDKNADTKLPNGANYGFPVSTMHIVANKAWAEKNPAAAKLF
                     AIMQLPVADINAQNAIMHDGKASEGDIQGHVDGWIKAHQQQFDGWVNEALAAQK"
     gene            24623..25807
                     /locus_tag="PN977_002017"
     CDS             24623..25807
                     /locus_tag="PN977_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDI6042046.1"
                     /translation="MTKPNHELSPALIVLMSIATGLAVASNYYAQPLLDTIARNFSLS
                     ASSAGFIVTAAQLGYAAGLLFLVPLGDMFERRRLIVSMTLLAAGGMLITASSQSLAMM
                     ILGTALTGLFSVVAQILVPLAATLASPDKRGKVVGTIMSGLLLGILLARTVAGLLASL
                     GGWRTVFWVASVLMALMALALWRGLPQMKSETHLNYPQLLGSVFSMFISDKILRTRAL
                     LGCLTFANFSILWTSMAFLLAAPPFNYSDGVIGLFGLAGAAGALGARPAGGFADKGKS
                     HLTTTFGLLLLLLSWLAIWFGHTSVLALVIGILVLDLTVQGVHITNQTVIYRIHPDAR
                     NRLTAGYMTSYFIGGAAGSLISASAWQHGGWAGVCLAGATIALVNLLVWWRGFHRQEA
                     AN"
     gene            25931..26668
                     /gene="ygaZ"
                     /locus_tag="PN977_002018"
     CDS             25931..26668
                     /gene="ygaZ"
                     /locus_tag="PN977_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine exporter subunit YgaZ"
                     /protein_id="HDI6042047.1"
                     /translation="MESPTPQPAPGSATFMEGCKDSLPIVISYIPVAFAFGLNATRLG
                     FSPLESVFFSCIIYAGASQFVITAMLAAGSSLWVAALTVMAMDVRHVLYGPSLRSRII
                     QRLQKSKTALWAFGLTDEVFAAATAKLVRNNRRWSENWMIGIAFSSWSSWVFGTVIGA
                     FSGSGLLQGYPAVEAALGFMLPALFMSFLLASFQRKQSLCVTAALVGALAGVTLFSIP
                     VAILAGIVCGCLTALIQAFWQGAPDEL"
     gene            26658..26993
                     /gene="ygaH"
                     /locus_tag="PN977_002019"
     CDS             26658..26993
                     /gene="ygaH"
                     /locus_tag="PN977_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine transporter subunit YgaH"
                     /protein_id="HDI6042048.1"
                     /translation="MSYEVLLLGLLVGAANYCFRYLPLRLRVGNARPTKRGAIGILLD
                     TIGIASICALLVVSTAPEVMHDTRRFVPTLVGFAVLGASFYKTRSIIIPTLLSALAYG
                     LAWKVMAII"
     gene            27084..27614
                     /gene="emrR"
                     /locus_tag="PN977_002020"
     CDS             27084..27614
                     /gene="emrR"
                     /locus_tag="PN977_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter EmrAB
                     transcriptional repressor EmrR"
                     /protein_id="HDI6042049.1"
                     /translation="MDSSFTPIEQMLKFRASRHEDFPYQEILLTRLCMHMQSKLLENR
                     NKMLKAQGINETLFMALITLESQENHSIQPSELSCALGSSRTNATRIADELEKRGWIE
                     RRESDNDRRCLHLQLTEKGHEFLREVLPPQHNCLHQLWSALSTTEKDQLEQITRKLLS
                     RLDQMEQDGVVLEAMS"
     gene            27741..28913
                     /gene="emrA"
                     /locus_tag="PN977_002021"
     CDS             27741..28913
                     /gene="emrA"
                     /locus_tag="PN977_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrA"
                     /protein_id="HDI6042050.1"
                     /translation="MSANAETQTPQQPVKKSGKRKRLLLLLTLLFIIIAVAIGIYWFL
                     VLRHFEETDDAYVAGNQIQIMSQVSGSVTKVWADNTDFVKEGDVLVTLDPTDARQAFE
                     KAKTALASSVRQTHQLMINSKQLQANIEVQKIALAKAQSDYNRRVPLGNANLIGREEL
                     QHARDAVTSAQAQLDVAIQQYNANQAMILGTKLEDQPAVQQAATEVRNAWLALERTRI
                     VSPMTGYVSRRAVQPGAQISPTTPLMAVVPATNMWVDANFKETQIANMRIGQPVTITT
                     DIYGDDVKYTGKVVGLDMGTGSAFSLLPAQNATGNWIKVVQRLPVRIELDQKQLEQYP
                     LRIGLSTLVSVNTTNRDGQVLANKVRSTPVAVSTAREISLAPVNKLIDDIVKANAG"
     gene            28930..30468
                     /gene="emrB"
                     /locus_tag="PN977_002022"
     CDS             28930..30468
                     /gene="emrB"
                     /locus_tag="PN977_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrB"
                     /protein_id="HDI6042051.1"
                     /translation="MQQQKPLEGAQLVIMTIALSLATFMQVLDSTIANVAIPTIAGNL
                     GSSLSQGTWVITSFGVANAISIPLTGWLAKRVGEVKLFLWSTIAFAIASWACGVSSSL
                     NMLIFFRVIQGIVAGPLIPLSQSLLLNNYPPAKRSIALALWSMTVIVAPICGPILGGY
                     ISDNYHWGWIFFINVPIGVAVVLMTLQTLRGRETRTERRRIDAVGLALLVIGIGSLQI
                     MLDRGKELDWFSSQEIIILTVVAVVAICFLIVWELTDDNPIVDLSLFKSRNFTIGCLC
                     ISLAYMLYFGAIVLLPQLLQEVYGYTATWAGLASAPVGIIPVILSPIIGRFAHKLDMR
                     RLVTFSFIMYAVCFYWRAYTFEPGMDFGASAWPQFIQGFAVACFFMPLTTITLSGLPP
                     ERLAAASSLSNFTRTLAGSIGTSITTTMWTNRESLHHAQLTESVNPFNPNAQAMYSQL
                     EGLGMTQQQASGWIAQQITNQGLIISANEIFWMSAGIFLVLLGLVWFAKPPFGAGGGG
                     GGAH"
     gene            complement(30532..31047)
                     /gene="luxS"
                     /locus_tag="PN977_002023"
     CDS             complement(30532..31047)
                     /gene="luxS"
                     /locus_tag="PN977_002023"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008458247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HDI6042052.1"
                     /translation="MPLLDSFTVDHTRMEAPAVRVAKTMNTPHGDAITVFDLRFCVPN
                     KEVMPERGIHTLEHLFAGFMRNHLNGNGVEIIDISPMGCRTGFYMSLIGTPDEQRVAD
                     AWKAAMEDVLKVQDQNQIPELNVYQCGTYQMHSLQEAQDIARNILERDVRINSNEELA
                     LPKEKLQELHI"
     gene            complement(31197..32753)
                     /gene="gshA"
                     /locus_tag="PN977_002024"
     CDS             complement(31197..32753)
                     /gene="gshA"
                     /locus_tag="PN977_002024"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HDI6042053.1"
                     /translation="MIPDVSQALAWLEKHPQALKGIQRGLERETLRVNADGTLATTGH
                     PEALGSALTHKWITTDFAEALLEFITPVDGDIEHMLTFMRDLHRYTARNMGDERMWPL
                     SMPCYIAEGQDIELAQYGTSNTGRFKTLYREGLKNRYGALMQTISGVHYNFSLPMAFW
                     QAKCGDIAGADAKEKISAGYFRVIRNYYRFGWVIPYLFGASPAICSSFLQGKPTSLPF
                     EKTECGMYYLPYATSLRLSDLGYTNKSQSNLGITFNDLYEYVAGLKQAIKTPSEEYAK
                     IGIEKDGKRLQINSNVLQIENELYAPIRPKRVTRSGESPSDALLRGGIEYIEVRSLDI
                     NPFSPIGVDEQQVRFLDLFMVWCALADAPEMSSSELACTRVNWNRVILEGRKPGLTLG
                     IGCETAQFPLPQVGKDLFRDLKRVAQTLDSINGGEAYQKVCDELVACFDNPDLTFSAR
                     ILRSMIDTGIGGTGKAFAEAYRNLLREEPLEILREEDFVAEREASERRQQEMEAADTE
                     PFAVWLEKHA"
     gene            complement(32826..33254)
                     /locus_tag="PN977_002025"
     CDS             complement(32826..33254)
                     /locus_tag="PN977_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HDI6042054.1"
                     /translation="MSEALSLFSLFASSFLSATLLPGNSEVVLVAMLLSGISHPWVLV
                     LTATMGNSLGGLTNVILGRFFPLRKTSRWQEKATGWLKRYGAVTLLLSWMPVVGDLLC
                     LLAGWMRISWGPVIFFLCLGKALRYVAVAAATVQGMMWWH"
     gene            complement(33251..33817)
                     /gene="yqaB"
                     /locus_tag="PN977_002026"
     CDS             complement(33251..33817)
                     /gene="yqaB"
                     /locus_tag="PN977_002026"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-1-phosphate/6-phosphogluconate
                     phosphatase"
                     /protein_id="HDI6042055.1"
                     /translation="MYERYAGLIFDMDGTILDTEPTHRKAWREVLGHYGLQYDVQAMI
                     ALNGSPTWRIAQAIIELNQADLDPHALAREKTEAVRSMLLDSVEPLPLVEVVKSWHGR
                     RPMAVGTGSESAIAEALLAHLGLRRYFDAVVAADHVKHHKPAPDTFLLCAQRMGVQPT
                     QCVVFEDADFGIQAARAAGMDAVDVRLL"
BASE COUNT         8392 a         8655 c         8565 g         8531 t
ORIGIN      
        1 gcgggcattt tttccaggta cttttgaata agtactgatg gataaataca ttgcagtggc
       61 gtgccacgta ccaaaacacc agccctcatt cgaaaccacc taccgcactg cttctttaaa
      121 atggcgttag tcgtgaaata ttgaccgcca tcgagtcccc cttgtaacct taactcttcc
      181 tgatacatga atgatgattt ggtggcccct gctggacttg aaccagcgac caggcgatta
      241 tgagtcgcct gctctaacca ctgagctaaa gggccttgag tatgcaataa caatacttat
      301 aaaccaagca ataaacatga tgattatatg atgtaaataa gagattttat gcgttctcat
      361 ccatcctggg tcgtaattac acgcgacata aatcccgaca ccgcttccat tcgcaaagtc
      421 gatactcgca gtcaacaagc atgttaataa taagcactat ctatagttat catcgattca
      481 atgatagttt gtaatgattt tgtatctaat aatataactt tattacatta gctgaagagt
      541 tttcgcatca tgatgatatc tgttactttt cactccataa aaataaactt catatagcaa
      601 tatattcttt tatagaactt attctgctaa tcattagttt cgtatgagcg atttttgaca
      661 gttgcctctc cagaccacat cgataattaa aaaaacagat ttaagcatta tccttttcca
      721 tatgaatatt ggataaaagt aggacatctg tttgcaatta ctttcacaac aattaaacat
      781 ttttatgttt ccgtatacac cttattactc taccattaga ggaactttat tatgttttct
      841 ataaaaccag gaccaagaaa tttacctatc gacaacccca cattgttatc atggaacatt
      901 actgacgggg atctaaattc caaattaaat acattagaat atctaaactg tataacaaat
      961 attattaatg cttgtggagt ttatcctcag gatttaaaag acagagaaat tatatcaact
     1021 tttcacgcag aaaaagtcat taatgatctg ttaaaaaacg attataaaat ttccctttct
     1081 ccagatacaa cttaccgaga gctgaataaa gctgcacagc gtagcattac agcgccagac
     1141 aggataggag aagggaaaac atgggtttat caacgagata caatggttga aagaggtgat
     1201 aacagcagcg ttcatcagta tggtccagct gaacatttca ctcacattat atctgacaaa
     1261 ccatccccaa aagataagta tgttgcatat gctattaaca ttcctgacta tgagctggca
     1321 gccgatgtat ataatattaa cgtgacgtca ccttccggac agcaagaaac atttaaaata
     1381 ctaatcaatc cagaacatct acggcaaaca cttgagcgta aatctcttac tgctgttcag
     1441 aaatcacaat gtgaaatcat cacccccaaa aaacctggcg aagcgattct tcatgctttt
     1501 aatgccacct accagcaaat cagggaaaat atgtctgagt ttgcacgttc ccattatggg
     1561 tatatacaaa ttcctccagt gacaactttc cgcgccgacg gaccagaaac tcccgaagaa
     1621 gaaaaaggtt actggtttca cgcttatcaa cccgaagatc tttgtaccat ccataatcca
     1681 atgggagatt tgcaggattt tatcgcattg gttaaagatg ctaaaaaatt tggtatcgat
     1741 atcattcctg attatacctt taactttatg ggaattgggg gtagtggtaa aaatgacctg
     1801 gattatccct ctgctgatat acgagcgaag atcagtaaag atatagaaag tggtatccct
     1861 ggctattggc aaggtcaggt tttgattcca tttactatag atccagtaac aaaagaacgt
     1921 aaacaaatcc atccagaaga tatacatctc actgcaaaag actttgaaac aagtaaagat
     1981 aacatctcta aggatgaatg ggaaaacctc catgcattaa aagaaaagcg tttaaatgga
     2041 atgcctaaaa caacccccaa aagtgaccag gttattatgt tgcaaaatca atacgttcgt
     2101 gaaatgcgaa aatatggcgt acgaggttta cgttacgatg cagcaaaaca ctcaaaacat
     2161 gaacaaatag aaagatcaat aactccaccg cttacaattt ataatgagcg attacacaat
     2221 actaacttat ttaagccaat atatcatgaa aaagccgtta tgaattacat ggaatacctg
     2281 gtaacttgtc agttggatga agaacaaatg tcatcgctgc tttatgaaag agatgattta
     2341 agcgccattg atttttcatt gctcatgaag acgataaaag ccttttcatt tggtggcgat
     2401 ctccaaaccc ttgcatcaaa accgggttca acaatctcaa gcatcccgtc aaaaagacgg
     2461 atattgatta acattaacca cgattttcct aacaatggca atcttttcaa tgactttcta
     2521 tttaaccatc aacaagatga acaattagca atggcatata tggccgctct cccgttcagc
     2581 aggcctttag tttactggga tggccaagta ttaaaatcaa cgactgaaat taaaaattat
     2641 gatgggtcga cgcgtgtcgg cggtgaggcg tggcttaata aaggttgctc tacctatcag
     2701 cagctctaca atgaattcca cgcattatat atagataaag caggaatatg gagcgcattt
     2761 gagggtgtat ttgcaactaa aaacgttctg gcctttagtc gtggggattc tgtgaacatt
     2821 aatcactctc ctcatgatgg actagttata ataaataaag gaaacgaaga agttgaaggt
     2881 acctggccta acaaattgca acctggaata tataaaaaca tggggagtaa tagcgttaac
     2941 attattatta ataatacccg aaaaattatc ccccctggta aagcatttat gcttagaggc
     3001 ggaactctaa atatcaatat tcctgggcgt agcgctcttc ttttagggaa aactggagaa
     3061 ccgccgaact atctctattt gtaatttaat gttatatctg ccccgctaaa acggggcaga
     3121 taatatgttt agtttactaa cggtcatttt gcagtgaagc catttaccgt tttttatcga
     3181 ccagaaaatc tgttctctga tggtaatcca cccctaacct gttgctttag ttattcattt
     3241 cctgtctcac tttgccttaa taccctacgt taaatgttac caatttgttg cttttgatca
     3301 caataagaaa acaatatggc gcatttgtgc gcatttttca gaaatgtaga tatttttaga
     3361 ttatggctac gaaatgagca tcgccatgtc accctacatc tcataagagg atcgcttctg
     3421 atgaatgcac tgaccgccgt acaaaataac gctgtcgatt caggccagga ctatagcgga
     3481 ttcactctca tcccgtcggc gcaatccccg cgtctgctgg aactcacctt caccgaacag
     3541 acgaccaaac agtttctcga acaggttgcc gaatggcccg tgcaggcgct ggagtacaaa
     3601 tcttttctgc gttttcgggt aggcaaaatt cttgacgatc tgtgtgcgaa ccagctgcaa
     3661 ccattgctgt tgaagaccct gctaaaccgt gctgaaggtg cgctgttgat caatgcggtg
     3721 ggtgtcgatg atgtgaagca agcggatgag atggtgaagc tggcaacggc ggtagcgcat
     3781 ctgattggcc gttccaattt cgatgcgatg agcggtcagt attacgcgcg ttttgtagtg
     3841 aaaaatgtcg ataactcaga cagctatctg cgtcagccgc accgcgtaat ggagctgcac
     3901 aacgacggca cctacgtcga agagatcacc gactatgtgc tgatgatgaa aatcgacgag
     3961 cagaacatgc agggtggaaa ttcgttgctg ctgcatctcg atgactggga acatctggac
     4021 cactatttcc gccacccgct ggcgcgtcgc ccgatgcgct ttgccgcgcc gccgagcaaa
     4081 aacgtcagca aagatgtttt ccatccagtg ttcgacgttg atcaacaggg tcgcccggtg
     4141 atgcgttata tcgaccagtt cgtccagcca aaagacttcg aagaaggcgt gtggctgagt
     4201 gagctttccg atgccattga aaccagcaaa ggcattcttt ctgtgcccgt tcccgttggc
     4261 aaattcctgt tgattaacaa tctattctgg ctgcacggtc gcgaccgctt tactccgcac
     4321 ccggatctgc gccgtgaact gatgcgtcag cgtggctatt tcgcttacgc cactaaccac
     4381 taccagacgc atcagtaagc gcaaaggaat tgagcggatg tatgattttg tgattattgg
     4441 cggcggcatc atcggcatgt cgaccgccat gcaactgatt gatgtttatc cggatgcccg
     4501 cattgcgttg ctggaaaaag agtccggccc ggcctttcat cagacgggcc acaacagcgg
     4561 cgtgatccat gccggggtct attacacgcc cggtagcctg aaggcacagt tttgcctggc
     4621 gggaaaccgc gccaccaaag ccttttgcga tcaaaacggc attcgctatg acaactgcgg
     4681 caagatgctg gtcgccacct ctgaactcga aatggaacgg atgcgcgcgt tgtgggaacg
     4741 cacggcggcg aacggtatcg agcgcgagtg gttaaacgcc gaggaactgc gcgagcgcga
     4801 accgaatatc accgggctcg gcggcatttt tgtgccgtcc agcggcattg tcagctatcg
     4861 cgaagtgacg gcggcgatgg caaaaatctt ccaggccaga ggcggcgaga ttatttataa
     4921 cgccgaagtc agcgccctca gtgagcataa aaacggcgtg gtgatacgta cccgtcaggg
     4981 cggtgaatat gaagcatcga cgctgattag ctgttccggg ctgatggctg accggctggt
     5041 gaaaatgctc ggcctcgaac cgggctttat tatctgcccg ttccgtggcg agtatttccg
     5101 ccttgcgccg gagcataacc agattgttaa ccacctgatt taccccattc ccgaccccgc
     5161 gatgccattt ttgggcgtgc atctcacccg catgatcgat ggcagcgtga ccgtcgggcc
     5221 aaacgcggtg ctggctttta aacgcgaagg ctatcgcaag cgcgatttct cgtttagtga
     5281 cacgctggag attttaggct cgtcggggat tcgccgggtg ctgcaaaacc atctacgctc
     5341 aggattgggc gagatgaaaa actcgctgtg caaaagcggc tatctgcggc tggtgcaaaa
     5401 gtattgtccc cggctttcgt taagcgatct ccagccctgg cccgcaggtg tgcgggcgca
     5461 ggcggtatcg ccggacggca agctgattga cgattttctg tttgtcacca ccccgcgcac
     5521 gatccacacc tgcaatgcgc cctccccggc agcgacatca gcaattccta ttggtgcgca
     5581 tattgtcagc aaggtacaaa cgctgttggc aagccagagt aaccccggac gcacgctgcg
     5641 agcggcacgt agtgtggatg ccttacacgc cgcatttaat caataacctt tgaaaacagg
     5701 acgtaacaat gaaacttaac gacagtaact tattccgcca gcaggcgttg attaacgggg
     5761 aatggctgga cgccaacaat ggcgaggtca tcgacgtcac caatccggcg aacggcgaca
     5821 agctgggtag cgtacccaaa atgggcgctg atgaaacccg cgccgctatc gacgccgcca
     5881 accgcgctct gcccgcctgg cgtgcgctca ccgccaaaga acgcgccaac attctgcgca
     5941 actggttcaa tttgatgatg gagcatcagg acgatttagc gcgcctgatg accctcgaac
     6001 agggcaaacc actggccgaa gcgaaaggcg aaatcagcta cgccgcctca tttattgagt
     6061 ggtttgctga agaaggcaaa cgtatttatg gcgacaccat tcccggtcat caggccgata
     6121 aacgcctgat tgttatcaag cagccgattg gcgtcaccgc ggctatcacg ccgtggaact
     6181 tcccggcggc gatgattacc cgtaaagcag gtccggcgct ggcggcaggc tgcacgatgg
     6241 tgctgaagcc cgccagtcag acgccgttct ctgcgctggc gctggcagaa ctggcgattc
     6301 gcgcgggcgt tccggctggc gtgtttaacg tggtcaccgg ttcggcgggc gcggtcggta
     6361 acgaactgac cagcaacccg ctggtgcgca agctgtcgtt taccggctcg accgaaattg
     6421 gccgccagtt aatggaacag tgcgcgaaag acatcaagaa agtgtcgctg gagctgggcg
     6481 gcaacgcgcc gtttatcgtc tttgacgatg ccgacctcga caaagccgtg gaaggcgcac
     6541 tggcctcgaa attccgcaac gccgggcaaa cctgcgtctg cgccaaccgt ttatacgtgc
     6601 agaacggcgt gtatgaccgc tttgccgaaa aattgcagca ggcggtgagc aaactgcaca
     6661 tcggcaacgg gctggagaaa ggcgtcacca tcgggccgct gatcgatgaa aaagcggtag
     6721 caaaagtgga agagcatatt gccgatgcgc tggagaaagg cgcgcgcgtg gtttgcggcg
     6781 gtaaagcaca tgaacgcggc ggtaacttct tccagccgac cattctggtg gacgttccgg
     6841 ctaacgccaa agtgtcgaaa gaagagacgt tcggccccct tgccccgcta ttccgcttta
     6901 aagatgaagc cgatgtgatt gcgcaggcca atgacaccga gtttggcctt gccgcctatt
     6961 tctacgcccg tgatttaagc cgcgtcttcc gcgtgggcga agcgctggag tacggcatcg
     7021 tcggcatcaa taccggcatt atttccaatg aagtggcccc gttcggcggc attaaagcct
     7081 cgggtctggg tcgtgaaggt tcgaagtatg gcatcgaaga ttacttagaa atcaaatata
     7141 tgtgcatcgg tctttaactg gagaacgcga atgagcagca ataaagagtt aatgcagcgc
     7201 cgcagtcagg cgattccccg tggcgttggg caaattcacc cgattttcgc tgaccgcgcg
     7261 gaaaactgcc gggtgtggga cgttgaaggc cgtgagtatc ttgatttcgc gggcgggatt
     7321 gcggtgctca ataccgggca cctgcatccg aaggtggtgg ccgcggtgga agcgcagttg
     7381 aaaaaactgt cgcacacctg cttccaggtg ctggcttacg agccgtatct ggagctgtgc
     7441 gagattatga atcagaaggt gccgggcgat ttcgccaaga aaacgctgct ggttacgacc
     7501 ggttccgaag cggtggaaaa cgcggtgaaa atcgcccgcg ccgccaccaa acgtagcggc
     7561 accatcgctt ttagcggcgc gtatcacggg cgcacgcatt acacgctggc gctgaccggc
     7621 aaggtgaatc cgtactctgc gggcatgggg ctgatgccgg gtcatgttta tcgcgcgctt
     7681 tatccttgcc cgctgcacgg cataagcgag gatgacgcta tcgccagcat ccaccggatc
     7741 ttcaaaaatg atgccgcgcc ggaagatatc gccgccatcg tgattgagcc cgttcagggc
     7801 gaaggcggtt tctacgccgc gacgccagcc tttatgcagc gtttacgcgc gctgtgtgac
     7861 gagcacggga tcatgctgat tgccgatgaa gtgcagagcg gcgcagggcg taccggcacg
     7921 ctgtttgcga tggagcaaat gggcgtggca ccagatctca ccacctttgc gaaatcgatc
     7981 gcaggcggtt tcccgctggc gggcgtcacc gggcgcgcgg aagtgatgga cgccgtcgca
     8041 ccgggcggac tgggtggcac ctatgccggt aacccgattg cctgcgtagc ggcgctggaa
     8101 gtgttgaagg tgttcgagca ggaaaatctg ctgcaaaaag ccaacgttct ggggcagaag
     8161 ctgaaagacg gattgctggc gatcgccgaa aaacacccgg agatcggcga cgtacgcggg
     8221 ctgggggcga tgatcgccat cgagctgttt gaagatggcg atcccagcaa accggacgca
     8281 aaactcaccg ccgagatcgt ggcacgcgcc cgcgataagg gtctgattct tctctcctgc
     8341 ggcccgtatt acaacgtgct gcgcatcctt gtaccgctca ccattgaaga cgctcagatc
     8401 cgtcagggtc tggagatcat cagccagtgt tttgctgagg cgaagctgta gcaccgcacc
     8461 atgccggagg cgacgctttg cggcttgtcc ggcctacgag aatccaggtc ggataaggcg
     8521 tttacgccgc atccgacaat ctgtacgtga acaggaataa atctatgtcg gccggataag
     8581 gcggagccgc tctccggcaa aaagaaccaa taacaattaa acgcgtgacc cgacgcggga
     8641 aatgtcgggg cgctctccca agtgacacac tttcgagagg attcaggatg gggcaatcat
     8701 cgcaaccaca tgagttaggc ggcgggctga agtcacgcca cgtcaccatg ttgtctattg
     8761 ccggtgttat cggcgcaagt ctgtttgtcg gttccagcgt cgccatcgcc gaagcgggcc
     8821 cggcggtatt actggcctat ctgttcgccg gattactggt ggttatgatt atgcggatgt
     8881 tggcggaaat ggcggttgcc acgcccgata ccggttcgtt ttccacctat gccgataaag
     8941 ccattggccg ttgggcgggc tataccatcg gctggttgta ctggtggttt tgggtactgg
     9001 ttatcccact ggaagccaac atcgccgcca tgatcctgca ctcgtgggtt ccaggcattc
     9061 ccatctggtt attttccctg gtcattaccc tcgccttgac tggcagtaat ttattaagcg
     9121 ttaaaaacta cggcgaattt gagttctggc tggcgctgtg caaagtcatc gctatcctgg
     9181 cctttatttt ccttggtgca gtcgcaatta gcggttttta cccttatgcc gaagtgagcg
     9241 ggatctcaag attgtgggat agcggcggct ttatgcccaa cggcttcggt gcggtattaa
     9301 gcgcgatgtt gatcaccatg ttctcgttta tgggcgcaga aattgtcact attgccgccg
     9361 cggaatctga cacgccggaa aaacatattg tccgcgccac caactcggtt atctggcgta
     9421 tttctatctt ctatttgtgc tctatttttg tcgtcgtggc attaataccg tggaatatgc
     9481 cggggctgaa agccgttgga tcttatcgct cggttctgga gttgctcaat attccccatg
     9541 cgaaattaat catggactgc gtgatattac tttccgtaac cagttgcctg aactcggcgc
     9601 tgtataccgc ttccagaatg ctctactcct taagccgtcg cggtgatgcg cccgcggtaa
     9661 tgggtaaaat caaccgcagt aaaaccccgt atgtggcggt attactctcc accggcgcgg
     9721 catttttaac ggtggtggtg aattattacg cgcctgcgaa agtgtttaaa tttctgatcg
     9781 acagctccgg tgctatcgcc ctgctggttt atttagtcat cgccgtttca caattgcgga
     9841 tgcgcaaaat tctgcgagca gaaggaagcg aaattcgcct gcggatgtgg ctttatccgt
     9901 ggctcacctg gctggtcatc ggctttatta cctttgtgtt ggtagtgatg ctattccgcc
     9961 cggcgcaaca gttagaagtg atctccaccg gcttattagc gatagggatt atctgtaccg
    10021 tgccaattat ggctcgctgg aaaaagctgg tattgtggca aaaaacaccc gttcataata
    10081 cgcgctgata atgatcagga gtcacaccat gaccattacg tctctggatg gctatcgctg
    10141 gctgaagaac gatattattc gcggtaattt tcaaccggat gaaaaattac ggatgagttt
    10201 gctgacatcg cgttatgcac ttggcgttgg gccgttacgg gaagctcttt cgcaactggt
    10261 ggcggaacgg ctggtcacgg tggtgaatca aaaagggtat cgggtggcgt ctatgtcaga
    10321 gcaggagctg ctcgatattt tcgacgcccg cgccaatatg gaagcgatgc tggtcagtct
    10381 ggcgattgcc cgcggtggcg atgaatggga ggcagacgtt ctcgcaaaag cgcatctgct
    10441 cagtaagctg gaggcctgtg acgccagtga gaaaatgctt gatgagtggg atcttcgtca
    10501 tcaggcgttt catactgcaa ttgtggcggg ctgtggttct cactatttgc tgcaaatgcg
    10561 tgaacggttg tttgatctgg cggcgcgcta tcgatttatc tggctgcggc gaacagtgct
    10621 ttcggtggag atgctggagg ataaacacga tcagcaccag accctgactg cggcggtact
    10681 ggcgcgagat accgcgcgcg ccagtgagtt aatgcgccag catttactga cgccaattcc
    10741 cattatccag caggcgatgg ctggcaatta actactcttc aggaatacgc aacacttgcc
    10801 ccgggtaaat tttatccggg ctttttagca tcggtttatt cgcttcgaag attttattgt
    10861 acagattggc gttaccgtag acctgtttgg aaatggcact cagagtgtcg ccagacttaa
    10921 cggtataaaa ctggctggca gcggctggtg tcgccgtttt cacctgatca tcgacactgg
    10981 caataccgga aatattcccc accgcaacaa ggattttctc cttcgcctcc tgactcaggc
    11041 catcacccgt gaccgtcgct ttgccgtcgg cgatttgaat gttcacttta tcggcatcag
    11101 gtataccggt tttgttcaga tgctcctgca ccttcttcgc ctgatcgtct ttatcgtgct
    11161 gacctgtaac cgcgtcccag agtttttctc cggcatcttt cacaaaattg aacagaccca
    11221 tagctacctc attcgttaac ggaaacactc agaaagtgta gcagaggaac gcggcgcgtt
    11281 aacgccgcgg gggtgaggac aggtttaatc gcgggtttgc acccagaaag cgtgaatcaa
    11341 accggggata tacccaagca gtgtcagcag aatattaata atgaacgccc aaccgaaccc
    11401 tttaccgagc agcacgccga gcggcggcag aatgatggtg atgacgattc tccagaaacc
    11461 catatgtact ccctataaga aaattactca ttgtttaaaa agagatttta tctcttaagc
    11521 gtagtaattt tagcggagcc tgccagtttc agcttacgct tttactctca tttacgctgc
    11581 tgatgctggc atatgttata tttttagttt agagttttct taatttagaa aatacttaaa
    11641 taactatgac tgaactcgcg caattgcagg ccagtgccga acaggcagcg gccttattga
    11701 aagcaatgag ccaccctaaa cggttgctga ttctgtgcat gcttagcggt tcccccggca
    11761 ccagtgcggg agagctgacg cgcattaccg gactgagtgc ctctgcgaca tcacagcatc
    11821 tcgctcgtat gcgggacgaa gggcttatcg acagacaacg ggatgcccaa cgcattctat
    11881 attccattaa aaatgaggcg gtaaatgcca ttatcgccac cctgaaaaat gtctattgtc
    11941 cgtaaggagt cactatggct ttgacaacca tttcgccgca tgatgcacaa gaattaatcg
    12001 cacgcggcgc aaagttaatc gatattcgtg acgctgatga atatcttcgt gaacatattc
    12061 ctgaagcaga tctggctcca ttatccgtgc tggaacagtc aggtcttccg gctaaattac
    12121 gtcgcgatca aattatattc cactgccagg caggtaaacg caccagtaat aacgccgata
    12181 aattagcagc gattgccgcc cccgcagaaa tctttttact cgaagatggg attgatggct
    12241 ggaaaaaagc aggattacta gtagcggtaa ataaatctca acccttgccg ttaatgcgcc
    12301 aggtgcagat cgctgcgggt ggtttaatat taatcggcgt tatactgggt tataccgtaa
    12361 atagcggttt cttcttatta agcggctttg ttggtgccgg gttactgttt gcaggaatta
    12421 gcggtttttg tgggatggca aggttgttag ataagatgcc gtggaaccaa cgagcttgag
    12481 aagcgacgcc ggacgcgcca tagcggcggc atccggcctc agtaattaga tcaggaaatc
    12541 gtcgagagat ttaccttctg ccagcgcctg agcgattggc ttcggtgtac ggccctgacc
    12601 ggtccaggtt ttagtttcac cgttaacatc ggtgaattta tatttcgccg gacgcggctg
    12661 gcgtttttta ccagcgcgtg gggcggccgc ggagctatta cccaataact cttccgggtt
    12721 aattccgtca gctttcatca gctccagcca ggtgctaatt ttttcctggc gctctgccag
    12781 ttcacgctgc tgctgttctt cttcttcacg tctttcttta gtgacaaccc tgaatttttc
    12841 gagcatttct tcaagaacgt caatggagaa ttcgcgagcc atcgcacgga gggtgcgaat
    12901 gttatttaaa ctttgtaaca ttacggacat aataaagaaa accttttaac gccaaaacaa
    12961 aaaagtattt cgtcgcgtag tgtaagcgat cacttattat atttccacaa aatttaatct
    13021 atttacagtc ctgcgcgaga ttatttcagc tgttattttc tgaaactctc agaataattt
    13081 caagattata tattagccat tttgatgagt aatttcggtt atttgtgagc agagcggcgc
    13141 tctctgctcg ctaattccgc aagcctgaca aaacaaaaat gaataaaaat gcaatttaac
    13201 cgaataaatg gaaacacccc tcactggtgc tttctgctgt caatgtacat tttctggcat
    13261 aaaaaatgct gaaaatgacc cggtcacgac atacaccgaa ttctctacgc ttatagcgtt
    13321 acctcacccc caaaccttca tgagtgataa taaaggtaaa cacatatcca attgatttaa
    13381 ttaaaaataa atcatatcgg catgtaaaaa tccacaatgt acaaaaaacg cgctgttacg
    13441 gaatatttta tctacaaaaa cagattaaat aaaaattttt cactaattga ttagtcatag
    13501 ccagcgatat acgctatgcg aaaatgcaga tggcaatgag atccactgct ttcatctcca
    13561 ttaacatccc attacgcttt tattaaggag cattagcatg ttctcaccgc agtcacgctt
    13621 gcgtcatgca gttgcagata cgttcgcgat ggttgtttac tgttctgtcg tgaacatgtg
    13681 tattgaagtt ttcctctccg gaatgagctt cgaacagtct ttttattcca gattggtagc
    13741 gattccggtg aacatcttaa ttgcatggcc atacggtatg taccgcgatc tgtttatgcg
    13801 cgcggcacgc aaagttagcc cgtcgggctg gataaaaaat ctggcggata tcctggctta
    13861 tgtgacgttc cagtcaccgg tgtatgtggc gatcttgtta gtggtgggcg cagactggca
    13921 tcagattatg gcggcagtca gttcaaacat cgttgtttcg atgttgatgg gggcggttta
    13981 tggctacttc ctcgattatt gccgccgact gtttaaagtc agccgttacc agcaggtaaa
    14041 agcctgaccc ttcttttcgc gactggcatc gccagtcgcg tctttaactg gcttcgccaa
    14101 ataaaccatt caaataacgt tcaagcgcaa cacgcgaact aaagccatgc gggataccgt
    14161 aatgctcatg acgttcaccc gctaaataga gcggaaattg ctggtaatga tcgcaagttt
    14221 taatatcgga agcgggttca gccagtgttg aactacgttc ttttaatgtt ggatgaatca
    14281 ccagggcggt acgtcccatt cgcgcttcgc gattaacata gacataattt tccccacggc
    14341 ggtaaccata cgctttctgg gttaccacat cgacagtaaa accgactttt tcaagtacgc
    14401 gcgccacctc gtctggtcgt aaatacatat ttgatcctcg ttattatcaa ccgcgggcct
    14461 accttacctg attgcgcatt cacaacgctt tcagaaaagt ccataaagcc gcgatgaacc
    14521 tcagttaaga aaattatggt ctacactgaa aattacatcg aattctaatg ggggatcata
    14581 tgtttaaccg accgaaccgc aacgacgttg atgatggcgt gcaagatatc cagaatgatg
    14641 tcaatcaatt agctgacagt ctcgaatctg tattgaagtc ctggtgcagc gacgccaaag
    14701 gggaagccga agccgctcgt agtaaagcgc aggctctact gaaagaaacc agggcgcgga
    14761 tgcatggtcg tactcgcgtc cagcaagccg cgcgcgatgc cgttggctgc gcggattctt
    14821 ttgttcgtga aagaccctgg tgtagtgtgg gtacagcggc agcggtgggt atttttatcg
    14881 gcgcactgtt aagcatgcgc aaatcgtaat gcaaaaatga taataaatac gcgtctttga
    14941 ccccgaagcc tggcttcggg gttttttagc ttaaggcttt acgtaattgt tgtgctacct
    15001 gtcgcgccat accttcgtta acgatattag taaacgaaag cagtaatcca ccttccccac
    15061 ttgaacggat acgccagtcg ctaagtgcct gcaccgcaag gcctgcagca ttggctttac
    15121 gcgcatgggc aatatcatcg cctatcattc tgatcaccat ctggatacca cctttctgcg
    15181 gcacaacctg aaatccttgc tgcgtcaacg cttgctcaat ccattgccga cgttgagcat
    15241 aatgctgacg cattttcttc agatgccgcc agaaatgccc ctcgcgaaga aagtctgcca
    15301 gtgtgttctg ccatagaaca ggtaccgcac atggagccag tgacgcctgg tggcggaatt
    15361 gtgcaatttg cttcaccggc accaccagcc acgcacagcg caatgcagga aatagcgctt
    15421 tgctgaatgt tccggcataa attacccgct gcggtgcgtc gagacttttc aacgccggta
    15481 acggcttacc gtgatagcga aactcactgt cataatcatc ttcaataatc catgcctgac
    15541 tacgatctgc ccattccagt atctgatgcc tgcgcgctaa agagagcgcc acacccagcg
    15601 gactttggtg tgctggtgtt atcagggcaa aacgcgcatc aggataattt tgtattccgc
    15661 ttgtgatatc cagtccattg tcatcaaccg gcacaggcaa aatttccaca tcatggcgag
    15721 tgacaatcgg gcgaatcagt ggaaagccgg gatcttctat ccacatcccg tttcccggtt
    15781 tcgccagcgc gtgcagaatt aaagctattg aggccgcata accgtgagtg ataaagacct
    15841 gctctggctg acaatcaatc ccacgtgaaa cgcgtaagta atcaactatt gcctcgcgca
    15901 acgccgcctc accgcagaca tcgcctaacg ccaaatcaaa gcgcgtctgg gtacgaaggc
    15961 gacgccccat cacccgcgcc cacagctctc gcggaaagag atcgagtgcg ggtaatccca
    16021 tttgaaatgg caacggttgc tgactttcac cggcaaaagc tacaacttgt acagtagatt
    16081 gttgcggata tatccgctca ctgacaaatg tgccagcctg tccccgcctg atcagccatc
    16141 cttgcgccac cagctcagca tacgcatttt cgacagtgga tcgagacacc cccagctcct
    16201 gcgaccaggt acgacttgaa ggcagccttg cgccgggttt cagttcccct tgctcaatag
    16261 ccgtttttaa ctgacgggcg atatcctgat agcgcggcat atggtctgct tttttaggta
    16321 atttatggct ctctttagca gtccattata gtgctacatt gcgggcgtta ataagaggag
    16381 aaactgtatg actacattac gtcagcctta ctacgaactt agcccggcag tgtataacgc
    16441 actagtgcag gccaaaacgg cactggaaaa tagcacactg gataccacgt tgatggagtt
    16501 agtttatttg cgcgtctcgc aaatcaacgg ctgcgcattt tgtctggaga tgcacagcaa
    16561 agcattgcgc aaatccggcg tgccacagca caaactggac gccctggcag gttggcgcgt
    16621 aagccatcat tttgatgaac gcgagcgggc ggcgctggca tgggcggaat cggtaaccga
    16681 aattgccaga acccatgcgg aagacgaggt ttatcagcct ttgcttgagc atttcagcgc
    16741 agcagagatc agcgacttaa cgtttgccat cgggctgatg aattgtttta accgtctggc
    16801 cgtttccatg cggatgtaac ttcttgatgc cgggtattcg cccggcaaaa aaacacaaaa
    16861 tcccctaccc cgttgagcca ttcatcggtg gcgtaatcaa tctaaaattc ccatatcttg
    16921 tgtggttgaa tcttttttca actacatcta gtatctctgt atcaacagag agacaacccg
    16981 acgcgtatca tcgcgccgta tcttcatttt aaacggaaat acgaatcatg cgcattacta
    17041 tttacactcg taacgattgc gttcagtgcc acgccaccaa acgggcgatg gaaaaccggg
    17101 gctttgattt tgaaatgatt aatgtcgatc gcgttcctga agcggcagaa gcgctgcgtg
    17161 ctcagggctt tcgtcagttg ccggttgtga ttgcaggcga tcttagctgg tctggtttcc
    17221 gcccggacat gatcaatcgt ctgcatccag cgccacacgc ggccagtgca tgagccagct
    17281 cgtctacttt tccagcagct ccgaaaatac gcagcgtttt atcgaacgtt tagggctgcc
    17341 cgcggtgcgc atcccgctca atgagcggga acggattcag gtagacgagc cttacatcct
    17401 gatcgtgccc tcttacggcg gcggcggtac ggctggcgcg gtgccacgac aggtcattcg
    17461 ctttttaaac gacgagcata accgggcgtt gctgcgcggc gttattgctt cgggtaatcg
    17521 caactttggt gaggcatatg gccgcgccgg agatgtgatt gcccggaaat gcggcgtgcc
    17581 gtggctgtac cgctttgaac tcatgggtac gcaaagcgat atcgaaaacg ttcgtaaagg
    17641 agtaaccgaa ttttggcaac gacaaccgca gaacgcctga cgcaggaaac gatggattat
    17701 cacgctctga atgcgatgct taacctttac gatagcgcag gtcgcattca gttcgataaa
    17761 gatcgccagg cagtagacgc ctttatgaca acgcatgtgc gtccgaacag tgtggccttc
    17821 agtagccagc agcagcgcct gaactggctg gttaacgaag gttactatga tgaaagcgtc
    17881 cttaatcgct actctcgcga ttttgtcatt acgctgtttg cccacgcgca cgccagcggt
    17941 tttcgtttcc agacattcct cggagcatgg aagttttaca ccagctatac gttgaagaca
    18001 ttcgacggta agcgttatct ggaagatttt gccgatcgcg tcacgatggt ggcgctgacg
    18061 ctggcacaag gcgatgaaac actagcgacg cagctcactg atgagatgct gtcaggacgc
    18121 tttcagccag ccacgccaac attcctcaac tgcggtaagc agcagcgcgg cgaactggtt
    18181 tcctgttttc tgctgcgtat tgaagacaat atggagtcga ttggccgggc agtaaattcc
    18241 gcactacaac tgtcgaaacg cggcggcggc gtagcatttt tgctgtcgaa tctgcgggaa
    18301 gcgggcgcgc caattaaacg tattgaaaat caatcttctg gcgtgattcc ggtgatgaaa
    18361 atgctggaag acgcattttc ctatgccaac caactcggcg cgcgtcaggg ggctggggca
    18421 gtctatttac atgctcatca tcccgatatt ctgcgttttc tcgatacaaa acgggaaaat
    18481 gccgacgaaa aaatccgcat taaaacactg tcgctgggcg tggtgatccc ggatatcact
    18541 ttccatctgg cgaaagagaa tgcgcagatg gcgctgtttt cgccctatga cgttgagcgg
    18601 gtttatggca agccgtttgc cgatgtcgcc atcagtgaac actatgacga actggttgcc
    18661 gatgaacgca ttcgcaaaaa atacctcaac gcccgtgatt tcttccagcg actggcagag
    18721 atccagtttg agtctggcta tccgtacatc atgtatgaag acacggttaa ccgtgctaac
    18781 cctatcgccg ggcgcataaa tatgagcaat ctctgctcag aaattttgca ggttaacagc
    18841 gcctcagatt atgacgagaa tctcgactat gcccgtacag gccatgatat ttcctgcaat
    18901 ttaggttcgt tgaatattgc ccacaccatg gattcccccg actttgcccg cacggtagag
    18961 actgccgtac gcggtttaac ggcggtgtca gatatgagtc atatccgcag tgtgccgtcc
    19021 atcgaagccg gaaatgccgc ctcacacgcc atcggtctgg ggcagatgaa tttacacggt
    19081 tatctggcgc gagaaggcat cgcttatggt tcgccggaag cactggattt caccaatctc
    19141 tatttctata ccatcacctg gcacgcactg cgtacctcga tgttgctggc gcgcgaacgc
    19201 ggtgaaacct tcgccgggtt caaacagtca cgctatgcca gtggtgaata tttcagccaa
    19261 tatctgcaaa gtaactggca gccgaaaacg gcgaaagttg gcgaactgtt tgcccgtagt
    19321 ggtgttacgt tacctacccg tgagatgtgg gcgcagctgc gcgacgacgt gatgtgctat
    19381 ggcatataca accagaatct ccaggcggtg cccccaaccg gttctatctc ttatatcaac
    19441 catgccacgt cgagtattca tccgattgtg gcgaaagtag agatacgcaa agagggcaaa
    19501 acaggacgcg tttactaccc tgccccgttt atgactaacg aaaatctggc gctgtatcag
    19561 gacgcttacg aaatcggcgc agaaaagatc atcgacacct acgcggaagc gactcgccat
    19621 gtcgatcagg ggctgtcgct gacgctgttt ttccccgata ccgccaccac tcgcgatatc
    19681 aacaaagcgc agatttacgc ctggcgcaag ggtatcaaaa cgctctatta cattcgcctg
    19741 cgtcagatgg cgctggaagg cactgaaatt gaaggctgcg tctcctgtgc actttaagga
    19801 atatctatga aactctctcg tatcagcgcc atcaactgga acaagatatc tgacgataaa
    19861 gatctggagg tgtggaatcg cctgaccagc aatttctggc taccagaaaa ggtgccactg
    19921 tcgaacgata tacctgcatg gcagacatta agtgtcgtag aacaacaact aacaatgcgc
    19981 gtttttactg gcctgacgct actcgacacg ctacaaaatg ttatcggcgc gccctcgctg
    20041 atgcccgatg cactcacccc ccatgaagag gcggtattat caaatatcag ctttatggaa
    20101 gcggttcatg cccgctctta cagttcaatt ttctcgacgc tgtgccagac caaagatgtc
    20161 gatgccgcct acgcctggag tgaagaaaac gcgccgttgc agcgaaaagc acagattatt
    20221 cagcaacatt atcgcggtga tgatccgctg aaaaagaaaa tcgccagtgt gtttcttgaa
    20281 tcttttttgt tctattccgg tttctggctg ccgatgtatt tttccagccg cggaaagcta
    20341 accaataccg cagacctgat ccgtctgatt atccgggatg aagcagtcca cggttactac
    20401 atcggctata aatatcagaa aaaactggaa aaaatctccc tggcacaacg tgaagagttg
    20461 aagagtttcg ccttcgattt gttgctggaa ctctatgaca acgagttgca atataccgat
    20521 gagctgtacg ccgaaacccc gtggtctgac gatgtaaaag cgtttctctg ttacaacgcc
    20581 aataaagcgt tgatgaatct gggctacgaa ccgctatttc ccgcagaaat ggcggaagtg
    20641 aatccggcaa tcctcgccgc gctttcgccg aatgccgatg aaaatcacga tttcttttcc
    20701 ggttcaggct cttcatatgt gatgggaaaa gcggtcgaaa cagaagatga agactggaat
    20761 ttctgagggg gttattttca aaaatatcac tacccgctgc agggaaataa ttcccgccaa
    20821 atagctttat atcacgcaaa taatttgtgg tgatctacac tgatactctg ttgcattatt
    20881 cgcctgaaac cacaatattc aggcgttttt ttgctatctt tgataaaaaa tatcaacttt
    20941 tctcgatttg ctctcagccc ttatatcatg ggaaattccg gcgatttgct cacatcaata
    21001 ttcatgccac atttgccatc aggggttgcc tcagattctc agtatgttag ggtagaaaaa
    21061 agtgactatt tccattgggt aatgtatcga cataaacaaa taacaggaat ctttctattg
    21121 catggcaatt aaattagaaa ttaaaaatct ttataaaata tttggtgagc atccacagcg
    21181 agctttcaaa tatatcgaac aaggactttc aaaagaacaa attcttgaaa aaactgggct
    21241 atcgcttggc gtaaaagacg ccagtctggc cattgaagaa ggcgagatat ttgtcatcat
    21301 gggattatcc ggttcgggta aatccacaat ggtacgcctt ctcaatcgcc tgattgaacc
    21361 cacccgcggg caagtgctga ttgatggtgt ggatattgcc aaaatatccg acgccgaact
    21421 ccgtgaggtg cgcagaaaaa agattgcgat ggtattccag tcctttgcct taatgccgca
    21481 tatgaccgtg ctggacaata ctgcgttcgg tatggaactg gccggaatta atgccgaagg
    21541 acgccgggaa aaagcccttg atgcactgcg tcaggtcggg ctggaaaatt atgcccacag
    21601 ctacccggat gaactctctg gcgggatgcg tcaacgtgtg ggattagccc gcgcgttagc
    21661 gattaatccg gatatattat taatggacga agccttctcg gcgctcgatc cattaattcg
    21721 caccgagatg caggatgagc tggtaaaatt acaggcgaaa catcagcgca ccattgtctt
    21781 tatttcccac gatctcgatg aagctatgcg tattggtgac cgaattgcca ttatgcaaaa
    21841 tggtgaagtg gtgcaggttg gtacaccgga tgaaattctt aataatccgg cgaatgatta
    21901 tgtccgtacc ttcttccgtg gcgtcgatat tagtcaggta ttcagtgcga aagatattgc
    21961 tcgccggaca ccgaacggct taattcgtaa aacccccggc ttcggcccgc gttcggcact
    22021 gaaattattg caggatgaag atcgcgaata tggctacgtc atcgaacgtg gtaataagtt
    22081 tgtcggcgca gtgtctatcg attcgcttaa aaccgcgtta acgcagcagc aaggtcttga
    22141 tgcggcgctg attgatgcgc cgttagcggt cgatgcacaa acgcctctta gcgagttgct
    22201 ctctcatgtc ggacaggcac cctgtgcggt gcccgtggtc gacgaggacc aacagtatgt
    22261 cggcattatt tcgaaaggaa tgctgctgcg cgctttagat cgtgaggggg taaataatgg
    22321 ctgatcaaaa taatccgtgg gataccacgc cagcggcgga cagtgctgca caatccgcag
    22381 acgcctgggg tacaccggcg actgcaccga ctgacggcgg tggcgctgac tggctgacca
    22441 gtacgcctgc gccaaacgtc gagcatttta atattctcga tccgttccat aaaacgctaa
    22501 tcccgctcga cagttgggtc actgaaggga tcgactgggt cgttacccat tttcgtcccg
    22561 tctttcaggg cgtgcgcctt ccggttgatt acatcctcaa cggtttccag caattgctgc
    22621 tgggtatgcc cgcgccggtg gcgattatcg ttttcgctct catcgcctgg cagatttccg
    22681 gggtcggaat gggcgtggcg acgctggttt cgctgattgc catcggcgca atcggtgcct
    22741 ggtcgcaggc catggttacc ctggcgctgg tgttaaccgc cctgctgttc tgtatcgtca
    22801 taggtttgcc gttggggatc tggctggcga gaagtccgcg agcggcgaaa attattcgtc
    22861 cactgcttga tgccatgcag accacgcccg cgtttgttta tctggtgcca atcgtcatgc
    22921 tgtttggtat cggtaacgtg ccgggcgtgg tggtgacaat catctttgcg ctgccgccga
    22981 ttatccgtct gacgattctg ggaattaacc aggttccggc ggatctgatt gaagcctcgc
    23041 gctcattcgg tgccagcccg cgccagatgc tgttcaaagt tcagttacca ctggcgatgc
    23101 caaccattat ggcgggcgtt aaccagacgc tgatgctggc cctttctatg gtggtcatcg
    23161 cctcgatgat tgccgtcggc gggctgggtc agatggtact tcgcggtatc ggtcgtctgg
    23221 atatggggct tgccaccgtt ggcggcgtcg ggattgtgat cctcgccatt atcctcgacc
    23281 gcctgacgca ggccgttggg cgcgactcac gcagtcgcgg caaccgtcgc tggtacacca
    23341 ctggccctgt cggtctgctg acccgcccat tcattaagta actctgcact tgcccggtga
    23401 cgccgggcat tatcaccctg ccaaaaaaag gaataacaat gcgacatagc gtactttttg
    23461 cgacagcgtt tgccacgctt atctctacac aaacttttgc tgccgatctg ccgggcaaag
    23521 gcattactgt taatccagtt cagagtacca tcactgaaga aaccttccag acgctgctgg
    23581 tcagtcgtgc actggagaaa ttaggttata ccgtcaataa acccagcgaa gtggattaca
    23641 acgttggcta cacctcactt gcttccggcg atgcaacctt caccgccgtg aactggacgc
    23701 cgctgcatga caacatgtat gaagctgccg gtggcgataa gaaattttat cgtgaaggag
    23761 tatttgttaa cggcgcggca cagggttacc tgatcgataa gaaaaccgcc gaccagtata
    23821 aaattaccaa catcgcgcaa cttaaagatc cgaagatcgc caaactgttc gataccaacg
    23881 gcgacggtaa agcggattta accggctgta acccaggctg gggctgcgaa ggtgcgatca
    23941 accaccagct tgccgcgtat ggactgacca ataccgtgac gcataatcag gggaactacg
    24001 cagcgatgat ggctgacacc atcagtcgtt acaaagaggg taagccggtg ttttactaca
    24061 cctggacgcc gtactgggtg agtaacgaac tgaaaccggg gaaagatgtg gtctggttgc
    24121 aggtgccgtt ctccgcactg ccgggcgata aaaatgccga taccaaactg ccgaatggcg
    24181 cgaattacgg cttcccggtc agtaccatgc atatcgttgc caacaaagcc tgggctgaga
    24241 aaaacccggc agcagcgaaa ctgtttgcca ttatgcagtt accagtggca gatattaacg
    24301 cccagaacgc cattatgcat gacggcaaag cctcagaagg cgatattcag ggccacgttg
    24361 atggttggat caaagcccac cagcagcagt tcgatggctg ggtgaatgaa gcgctggcag
    24421 cgcagaagta atttttattc gggcggataa ggcgtttttg cctcatccgc cgctctgtac
    24481 acaatgcctg atgcgacgct ggtgcgtctt atcataccta caaaccacac cgcacatttc
    24541 aacaatctat ctttcatccc atattcatca acatccgcta ttattgattt ccagcttaat
    24601 catcacctga tgaacaaaaa taatgactaa acctaatcat gagcttagcc cggcactgat
    24661 cgtgctgatg tctatcgcca ccggtctggc ggtagccagt aactattacg cccagccatt
    24721 actcgacacc atcgcgcgta acttttccct ttccgccagt tcggcgggct ttattgttac
    24781 cgccgcgcag ttgggctatg ccgcaggtct gctgtttctt gttcccctcg gtgatatgtt
    24841 tgaacgccgc cgcctgattg tctcgatgac cttactggca gcgggcggta tgttgattac
    24901 cgccagcagt cagtcgctgg cgatgatgat cctcggtacg gcattaaccg gtttattctc
    24961 agtcgtggca caaattctgg ttccgctggc agcgacgctg gcttcaccgg acaaacgcgg
    25021 caaagtggtc ggcaccatta tgagcgggct gctgttgggg atcttgctgg cacggaccgt
    25081 tgccggattg ctggcgagtc tcggtggctg gcgaactgtc ttttgggtcg cttcggtatt
    25141 aatggcactg atggcgctgg cgttatggcg tggtctgcca caaatgaaat cagaaaccca
    25201 cctcaactac ccacagttgc taggttctgt tttcagcatg tttatcagcg ataaaatcct
    25261 gcgcacccgc gcgttgctgg gctgcctgac ctttgccaac ttcagcattc tctggacctc
    25321 aatggccttt ttgcttgccg ctccaccttt taactacagc gatggcgtaa ttggtctgtt
    25381 cggacttgca ggagctgccg gggcgttagg cgctcgtccg gcgggcggtt ttgccgataa
    25441 gggcaaatca cacctcacca caactttcgg cctgctgctg ctgttacttt catggctggc
    25501 tatctggttt ggacacactt ctgtactggc gctggttatc ggcatcctgg tgctggacct
    25561 caccgtgcag ggcgtgcata tcactaacca gacggtaatt tatcgaatac accctgatgc
    25621 gcgtaatcgc ctgaccgcag gttacatgac cagctacttt attggcggtg ccgccggttc
    25681 gctaatttca gcctcagcct ggcaacatgg cggttgggct ggcgtttgtc tggctggcgc
    25741 gacgattgcc ctggttaact tactggtctg gtggcgaggt tttcatcgtc aggaagccgc
    25801 aaattaagca atcagcaacc gttttcgtgg ctttacactt ataagggtgt taagaagccc
    25861 atcagtctga taaggttaag atattcattc agtctattta taatattaac aatcgttaag
    25921 cgtacactct atggaaagcc ctactccaca gcctgctcct ggttcggcga ccttcatgga
    25981 aggatgcaaa gacagtttac cgattgttat tagttatatt ccggtggcct ttgcgttcgg
    26041 tctgaatgcg acccgtctgg gattctctcc tctcgaaagc gtttttttct cctgcatcat
    26101 ttatgcaggc gcgagccagt tcgtcattac cgcgatgctg gcagccggga gtagtttgtg
    26161 ggttgctgca ctgaccgtca tggcaatgga tgttcgccat gttttgtatg gcccgtcact
    26221 gcgtagccgt attattcagc gtctgcaaaa atcgaaaacc gccctgtggg cgtttggcct
    26281 gacggatgag gtttttgccg ccgcaaccgc aaaactggta cgcaataatc gccgctggag
    26341 cgaaaactgg atgatcggca ttgccttcag ttcatggtca tcgtgggtat ttggtacggt
    26401 aataggggca ttctccggca gcggcttgct gcaaggatat cccgccgttg aagcagcatt
    26461 aggttttatg cttccggcac tctttatgag tttcctgctc gcctctttcc agcgcaaaca
    26521 atctctttgc gttaccgcag cgttagttgg tgcccttgca ggcgtaacgc tattttctat
    26581 tcccgtcgcc attctggcag gcattgtctg tggctgcctc actgcgttaa tccaggcatt
    26641 ctggcaagga gcgcccgatg agctatgagg ttctgctgct tgggttactg gttggcgcgg
    26701 cgaattattg cttccgctat ctgccgctgc gcctgcgtgt gggtaatgcc cgcccaacca
    26761 aacgtggcgc gataggcatt ttgctcgaca ccattggcat cgcctcgata tgcgctctgc
    26821 tggttgtctc taccgcacca gaagtgatgc acgatacacg ccgtttcgtg cccacgctgg
    26881 tcggcttcgc ggtactgggt gccagtttct ataaaacacg cagcattatc atcccaacac
    26941 tgcttagtgc gctggcctat gggctagcct ggaaagtgat ggcgattata taacccacaa
    27001 gaatcatttt tctaaaacaa tacatttact ttatttgtca ctgtcgttac tatatcgtct
    27061 gaaatcaatg aggtcatacc caaatggata gttcgtttac gcccattgaa caaatgctaa
    27121 aatttcgcgc cagccgccac gaagattttc cttatcagga gatccttctg acccgtcttt
    27181 gcatgcatat gcaaagcaag ctgctggaaa accgcaataa aatgctgaag gctcagggga
    27241 ttaacgagac gttgtttatg gcgttgatta cgctggagtc tcaggaaaac cacagtattc
    27301 agccttctga attgagttgt gctcttggat catcccgtac caacgcgacg cgtattgccg
    27361 atgaactgga aaaacgcggt tggatcgaac gtcgtgaaag cgataacgat cgccgctgcc
    27421 tgcatctgca attaacggaa aaaggtcacg agtttttgcg cgaggtttta ccaccgcagc
    27481 ataactgcct gcatcaactc tggtccgcgc tcagcacaac agaaaaagat cagctcgagc
    27541 aaatcacccg caaattgctc tcccgtctcg accagatgga acaagacggt gtggttctcg
    27601 aagcgatgag ctaacgcgtc atctcgctca aaaatccaga tttataaaag aaaaaatgac
    27661 tggccagcat cgcaacatgc tggccttttt ggcaagcagg tcggctcagc cgatgagtta
    27721 agaagatcgt ggagaacaat atgagcgcaa atgcggagac tcaaaccccg cagcaaccgg
    27781 taaagaagag cggcaaacgt aagcgtctgc tcctccttct caccttgctc tttataatta
    27841 ttgccgtagc gatagggatt tattggtttt tagtactgcg tcatttcgaa gaaaccgatg
    27901 atgcatacgt ggcagggaat cagattcaaa ttatgtctca ggtgtctggc agcgtgacga
    27961 aagtctgggc cgataacacc gattttgtaa aagaaggcga cgtgctggtc actctcgacc
    28021 cgacagatgc tcgccaggcg tttgaaaaag ccaaaactgc actggcttcc agcgttcgcc
    28081 aaacccacca gctgatgatt aacagcaaac agttgcaggc gaatattgag gtgcaaaaaa
    28141 tcgcactcgc gaaagcacaa agcgactaca accgccgtgt gccgctgggc aatgccaacc
    28201 tgattggtcg cgaagagctg caacacgccc gcgacgccgt taccagtgcc caggcacaac
    28261 tggacgtcgc tattcaacaa tacaatgcaa atcaggcgat gattctgggg acgaaactgg
    28321 aagatcagcc tgccgtgcaa caggctgcca ccgaagtacg taacgcctgg ctggcgctgg
    28381 agcgtactcg tattgtcagt ccgatgaccg gttatgtctc ccgccgcgcg gtacagcctg
    28441 gggcgcaaat tagcccaacg acgccgctga tggcggtcgt tccagccacc aatatgtggg
    28501 tggatgccaa ctttaaagag acgcagattg ccaatatgcg tatcggtcaa ccggtcacca
    28561 tcaccaccga tatttacggc gatgatgtga aatacaccgg taaagtggtt ggtctggata
    28621 tgggcacagg tagcgcgttc tcactgcttc cggcgcaaaa cgccaccggt aactggatca
    28681 aagtcgttca gcgtctgcct gttcgtattg aactggacca gaaacagctc gagcaatacc
    28741 cgctgcgtat cggtttgtcc acgctggtga gcgtcaatac cactaaccgt gacggtcagg
    28801 tactggcaaa taaagtacgt tccacaccgg tagcggtaag caccgcgcgt gaaatcagct
    28861 tggcacctgt caataaactg atcgacgata tcgtaaaagc taacgctggc taatccagag
    28921 gtgcgtgtga tgcaacagca aaaaccgctg gaaggcgcgc aactggtcat tatgacgatt
    28981 gcgctgtcac tggcgacatt catgcaggtg ctggactcca ccattgctaa cgtggcgatc
    29041 cccactatcg ccgggaatct gggctcatcg ctcagccagg gaacgtgggt aatcacttct
    29101 ttcggggtgg cgaatgccat ctcgatcccg cttaccggct ggctggcaaa gcgcgtcggg
    29161 gaagtgaaat tgttcctctg gtcaaccatc gcctttgcta ttgcgtcgtg ggcgtgtggt
    29221 gtctccagca gcctgaatat gctgatcttc ttccgcgtga ttcaggggat tgtcgccggg
    29281 ccgctgatcc cgctttcgca aagtctattg ctgaataact acccgccagc caaacgctcg
    29341 atcgcgctgg cgttgtggtc gatgacggtg attgttgcgc caatttgcgg tccgatcctc
    29401 ggcggttata tcagtgataa ttaccactgg ggctggatat tcttcatcaa cgtgccgatt
    29461 ggcgtggcgg tggtgttgat gacactgcaa actctgcgcg gacgtgaaac ccgcaccgaa
    29521 cggcggcgga ttgatgccgt ggggctggca ctgctggtta ttggtatcgg cagcctgcag
    29581 attatgctcg accgcggtaa agagctggac tggttctcat cacaggaaat tatcatcctc
    29641 accgtggtgg cggtggtggc tatctgcttc ctgattgtct gggagctgac cgacgataac
    29701 ccgatagtcg atctgtcgtt gtttaagtcg cgcaacttca ccatcggctg cttgtgtatc
    29761 agcctcgcgt atatgctcta cttcggcgcg attgttctgc tgccgcagtt gttgcaggag
    29821 gtctacggtt acacggcgac gtgggcaggg ctggcctctg caccggtagg gattattccg
    29881 gtgatcctgt cgccgattat cggccgcttc gcgcataaac tggatatgcg ccgactggta
    29941 accttcagct ttattatgta tgccgtctgc ttctactggc gtgcctatac ctttgaaccg
    30001 ggtatggatt ttggcgcgtc ggcctggccg cagtttatcc agggttttgc ggtggcctgc
    30061 ttctttatgc cgctaaccac cattacgctg tccggtttgc caccggaacg actggcggca
    30121 gcatcgagcc tctccaactt tacgcgaacg ctggcggggt ctatcggcac atcgataacc
    30181 acgactatgt ggaccaaccg cgagtcgctg caccatgcgc agttgactga gtcggtaaac
    30241 ccgttcaacc cgaatgccca ggcgatgtac agtcaactgg aagggcttgg gatgacgcaa
    30301 cagcaggcat caggctggat tgcccagcag atcaccaatc aggggctgat tatttccgcc
    30361 aatgagatct tctggatgtc agccgggata ttcctcgtct tgctggggct ggtgtggttt
    30421 gctaaaccgc catttggcgc aggtggcggc ggaggcggtg cgcactaagt acaactaagc
    30481 cagttcattt gaactggctt ttttcaatta attgtgaaga tagtttactg actagatgtg
    30541 cagttcctgc aacttctctt tcggcagcgc cagttcttcg ttgctgttga tgcgcacgtc
    30601 acgttccaga atgttacgcg caatatcctg cgcttcctgc aacgagtgca tctggtaagt
    30661 gccacactgg tagacgttca gttccgggat ctgattctga tcctgcactt tcagcacgtc
    30721 ttccattgcc gctttccagg catcagcgac acgctgctca tctggcgtac caatcagact
    30781 catataaaaa ccggtgcggc agcccattgg cgagatatcg ataatctcta cgccattacc
    30841 gttaagatgg ttacgcataa aaccagcaaa caggtgctcc agggtatgga tccctctttc
    30901 tggcatcact tctttgttcg gcacgcagaa gcgcagatcg aacacggtga ttgcgtcgcc
    30961 atgcggggtg ttcattgttt tcgccacccg aactgcaggc gcttccatcc gggtatgatc
    31021 gactgtgaag ctatctaaca acggcattta gccacctccg gtaatttttt taaaaatttt
    31081 ctgaactctt tcttcccagg cgagtctgag tatatgaaag acgcgcattt gttatcatca
    31141 tccctgaatt cagagatgaa attttggcca ctcacgagtg gcctttttct tttctgtcag
    31201 gcgtgttttt ccagccacac cgcaaacggt tcggtatcag cggcttccat ttcctgctga
    31261 cggcgttcag acgcctcgcg ctcggcgaca aaatcctctt cgcgcagaat ttccagcggc
    31321 tcttcacgca gcagattacg gtacgcttca gcaaacgctt tgcctgttcc gccaatacca
    31381 gtatcaatca tagaccttaa gatacgggca gagaaagtca gatcgggatt atcgaagcag
    31441 gcgaccagtt catcacacac tttctgatac gcttcgccgc cgttaatgct atccagcgtt
    31501 tgcgcgacgc gtttcagatc gcggaacaga tctttaccca cctgcggtaa cgggaactgc
    31561 gcggtttcgc agccgatacc cagcgtcagg cccggtttgc gaccttcgag gatcacccgg
    31621 ttccagttaa cgcgtgtaca ggcaagttcg ctactgctca tttccggtgc atcagccagc
    31681 gcacaccaga ccataaacag gtcgaggaat cgcacctgct gttcatctac accaatcggc
    31741 gagaatgggt tgatgtccag cgaacgcact tcaatatatt caatgccgcc acgtaacagc
    31801 gcatcagaag gcgactcgcc gctgcgggta acgcgttttg gacgaatcgg cgcgtacagt
    31861 tcgttttcaa tctgcagcac gttgctgttg atttgcagcc tcttaccatc tttctcaatg
    31921 ccaatcttcg cgtactcttc cgatggcgtt ttgattgcct gtttaaggcc cgctacgtac
    31981 tcgtaaagat cgttgaaggt aataccaaga ttgctttgcg atttattggt atagccgaga
    32041 tcactcaaac gaagagaggt cgcatacggc aggtaataca taccgcactc ggttttctca
    32101 aacggcagcg atgttggttt tccttgcagg aaagaagaac aaatcgccgg agacgcgcca
    32161 aacagataag gaatgaccca accgaaacga tagtaattgc ggataacgcg gaaatagccc
    32221 gcagaaattt tctctttggc atcagcgccc gcgatatcac cacacttcgc ttgccagaat
    32281 gccattggca aagagaaatt gtagtgcacg ccggaaatgg tttgcatcag cgcgccgtag
    32341 cgatttttca gcccttcacg gtacagcgtt ttaaagcgtc cagtgttaga agtgccgtac
    32401 tgtgccagtt cgatgtcctg accttctgcg atgtagcatg gcatacttaa cggccacatc
    32461 cgctcatcgc ccatattgcg cgccgtataa cgatgcagat cgcgcataaa ggtcaacata
    32521 tgttcaatat caccatccac tggtgtaatg aattccagca atgcttccgc aaaatcggta
    32581 gtaatccatt tgtgcgtcag tgcggaacct aatgcttcag gatgacctgt tgttgccaat
    32641 gtgccatcag cattaacacg caaagtttcg cgctccagcc cacgctgtat cccctttaac
    32701 gcctgaggat gtttttccag ccaggccagc gcctgtgata cgtccgggat caaattgacc
    32761 tcccgcctgt caaaatcgtt ttaattagca taactgtaat ggtgaccata tgtgcaggcc
    32821 tacaattagt gccaccacat catgccctga acggtagctg ctgcaactgc aacatagcgt
    32881 aacgctttac caaggcacaa aaaaaagatt accggtcccc acgagatgcg catccatccc
    32941 gctaacagac acagtaaatc gccaaccacg ggcatccagc ttaataatag cgtgactgca
    33001 ccatagcgtt tcagccagcc ggtagctttc tcttgccagc gcgatgtttt acgcaatgga
    33061 aagaaacgcc caaggataac gttagttaac cctccaaggc tattacccat tgttgctgtt
    33121 aaaactaaaa cccagggatg actgatcccg gaaagcaaca ttgccaccag cacgacttcg
    33181 gagttgccgg gtaatagtgt agcgctgaga aaactactgg caaacaacga gaaaagcgat
    33241 aacgcttcac tcacagcaag cgaacatcca cagcgtccat gcctgctgca cgggccgcct
    33301 gaataccgaa atcggcatct tcaaagacca cacactgtgt cggttgcacg cccatacgct
    33361 gcgcgcacaa caaaaatgtg tctggcgcgg gtttatggtg tttgacgtga tcggcagcga
    33421 cgacggcgtc aaaataacgg cgtaatccca ggtgcgccag caatgcctca gcgatggcgc
    33481 tttcactccc cgttcctaca gccattgggc gacgaccatg ccaacttttc accacttcaa
    33541 caagaggaag cggctcgaca ctatccagca gcatacttct tactgcttct gttttttcac
    33601 gcgctaacgc atgcgggtcg agatcggcct gattcagctc aataattgcc tgagcaatac
    33661 gccaggtggg cgagccatta agcgcaatca tcgcctgaac atcgtactga agaccgtagt
    33721 gccctaatac ttcgcgccac gctttacggt gcgtaggctc ggtatccagg atggtgccat
    33781 ccatatcaaa aattaaacct gcataacgct cgtacatggt cttctcgcaa gtcgaaaaat
    33841 atgacgctac tttagcgtaa ttgcttgatt ttgtcgctga tggcgaagga gaagaaaatt
    33901 caggaaatgg ataaagtagt tatttagaaa tgagatattt ttgaaggaaa ttttttggag
    33961 aagatggtgc atccgggagg atgactcact ggcgttcgcc cttcgggccg ttgctggccg
    34021 gcgttatcct ccctggtgcc tgcggttaat accgctacct cttcgatatc ttcattgctg
    34081 aagattacgg agaagatggt gcatccggga ggattcgaac ctccgaccgc tcggttcgta
    34141 gcc
//
LOCUS       DAMZFT010000040        33982 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.040, whole genome shotgun sequence.
ACCESSION   DAMZFT010000040 DAMZFT010000000
VERSION     DAMZFT010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 33982)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33982)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33982
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.040"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(<1..1498)
                     /gene="thiP"
                     /locus_tag="PN977_002027"
     CDS             complement(<1..1498)
                     /gene="thiP"
                     /locus_tag="PN977_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine/thiamine pyrophosphate ABC transporter
                     permease ThiP"
                     /protein_id="HDI6042056.1"
                     /translation="MATRRQPLIPGWLIPGVSAATLVVAVALAAFLALWWNAPQGDWV
                     AVWQDSYLWHVVRFSFWQAFLSALLSVVPAIFLARALYRRRFPGRLALLRLCAMTLIL
                     PVLVAVFGILSVYGRQGWLASLWQSLGLEWTFSPYGLQGILLAHVFFNLPMASRLLLQ
                     ALENIPGEQRQLAAQLGMRGWHFFRFVEWPWLRRQIPPVAALIFMLCFASFATVLSLG
                     GGPQATTIELAIYQALSYDYDPARAAMLALIQMVCCLGLVLLSQRLSKAIAPGTTLLQ
                     GWRDPDDRLHSRICDTVLIVLALLLLLPPLLAVIVDGVNRQLPEVLAQPVLWQALWTS
                     LRIALAAGVLCVVLTMMLLWSSRELRARQKMLAGQALEMSGMLILAMPGIVLATGFFL
                     LLNNTIGLPQSADGIVIFTNALMAIPYALKVLENPMRDITARYNMLCQSLGIEGWSRL
                     KVVELRALKRPLAQALAFACVLSIGDFGVVALFGNDDFRTLPFYLYQQI"
     gene            complement(1474..2457)
                     /gene="thiB"
                     /locus_tag="PN977_002028"
     CDS             complement(1474..2457)
                     /gene="thiB"
                     /locus_tag="PN977_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter substrate binding
                     subunit"
                     /protein_id="HDI6042057.1"
                     /translation="MLKKYLPLLLLCTAPVFAKPVLTVYTYDSFAADWGPGPVVKKAF
                     EADCNCELKLVALEDGVSFLNRLRMEGKNSKADVVLGLDNNLLDAASKTGLFAKSGVA
                     ADAVNVPGGWNNDTFVPFDYGYFAFVYDKNKLKNPPQSLKELVESDQNWRVIYEDPRT
                     STPGLGLLLWMQKVYGDNAPQAWQKLAKKTVTVTKGWSEAYGLFLKGESDLVLSYTTS
                     PAYHILEEKKDNYAAANFSEGHYLQVEVAARTAASKQPELAQKFLQFMVSPAFQNAIP
                     TGNWMYPVANVTLPAGFEQLTKPATTLEFTPAEVAAQRQAWISEWQRAVSR"
     regulatory      complement(2488..2587)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            2828..3397
                     /locus_tag="PN977_002029"
     CDS             2828..3397
                     /locus_tag="PN977_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III effector"
                     /protein_id="HDI6042058.1"
                     /translation="MKVRNPEQISISASNTTKDPGLTHSQIMRMFNLVKKTENMNLFE
                     ALWETLRNLFRSDKHSQAAARQILKDAFYFHNSDDYSKYFTGAVDGDARDKFTHWLIN
                     FNKLKEHAIDPENMAAKASLSPKDTLRVSFFIGDEAIFTLELQMKENTRTGCIDLSNA
                     YFNGVVICGIDRLEVDLSNAEMNNSRWYD"
     gene            complement(3627..5285)
                     /gene="sgrR"
                     /locus_tag="PN977_002030"
     CDS             complement(3627..5285)
                     /gene="sgrR"
                     /locus_tag="PN977_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SgrR"
                     /protein_id="HDI6042059.1"
                     /translation="MPSARLQQQFIRLWQCCEGKSQDTTLNELAALLSCSRRHMRTLL
                     NTMQDRGWLTWEAEVGRGKRSRLTFLYTGLALQQQRAEDLLEQDRIDQLVQLVGDKAT
                     VRQMLVSHLGRSFRQGRHILRVLYYRPLRNLLPGSALRRSETHIARQIFSSLTRINEE
                     NGELEADIAHHWQQISPLHWRFFLRPGVHFHHGRELEMDDVIASLKRINTLPLYSHIA
                     EIVSPTPWTLDIHLTQPDRWLPLLLGQVPAMILPREWETLSNFASHPIGTGPYAVIRN
                     STNQLKIQAFDDFFGYRALIDEVNVWVLPEIADEPAGGLMLKGPQGEEKEIESRLEEG
                     CYYLLFDSRTHRGANQQVRDWVSYVLSPTNLVYFAEEQYQQLWFPAYGLLPRWHHARP
                     THCEKPAGLESLTLTFYQDHIEHRVIAGIMQQILASHQVTLEIKEISYDQWHEGEIES
                     DIWLNSANFTLPLDFSLFAHLCEVPLLQHCLPIDWQADAARWRNGEMNLANWCQQLVA
                     SKAMVPLIHHWLIIQGQRSMRGLRMNTLGWFDFKSAWFAPPDPE"
     gene            5374..5505
                     /gene="sgrT"
                     /locus_tag="PN977_002031"
     CDS             5374..5505
                     /gene="sgrT"
                     /locus_tag="PN977_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001248776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake inhibitor SgrT"
                     /protein_id="HDI6042060.1"
                     /translation="MRQFYQHYFTATAKLCWLRWLSVPQRLTMLEELMQWEGSHSDY"
     gene            complement(5613..6218)
                     /gene="leuD"
                     /locus_tag="PN977_002032"
     CDS             complement(5613..6218)
                     /gene="leuD"
                     /locus_tag="PN977_002032"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HDI6042061.1"
                     /translation="MAEKFIKHTGLVVPLDAANVDTDAIIPKQFLQKVTRTGFGAHLF
                     NDWRFLDEKGQQPNPDFVLNFPQYQGASILLARENFGCGSSREHAPWALTDYGFKVVI
                     APSFADIFYGNSFNNQLLPVKLSDAEVDELFALVKANPGIHFDVDLEAQEVKAGEKTY
                     RFTIDAFRRHCMMNGLDSIGLTLQHDDAIASYEEKQPAFMR"
     gene            complement(6229..7629)
                     /gene="leuC"
                     /locus_tag="PN977_002033"
     CDS             complement(6229..7629)
                     /gene="leuC"
                     /locus_tag="PN977_002033"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HDI6042062.1"
                     /translation="MAKTLYEKLFDAHVVYEAENETPLLYIDRHLVHEVTSPQAFDGL
                     RAHGRPVRQPGKTFATMDHNVSTQTKDINACGEMARIQMQELIKNCKEFGVELYDLNH
                     PYQGIVHVMGPEQGVTLPGMTIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQTLKQ
                     GRAKTMKIEVQGKAAPGITAKDIVLAIIGKTGSAGGTGHVVEFCGEAIRDLSMEGRMT
                     LCNMAIEMGAKAGLVAPDETTFNYVKGRLHAPKGKDFDDAVAYWKTLQTDEGATFDTV
                     VTLQAEEISPQVTWGTNPGQVISVNDNIPDPASFADPVERASAEKALAYMGLKPGIPL
                     TEVAIDKVFIGSCTNSRIEDLRAAAEIAKGRKVAPGVQALVVPGSGPVKAQAEAEGLD
                     KIFIEAGFEWRLPGCSMCLAMNNDRLNPGERCASTSNRNFEGRQGRGGRTHLVSPAMA
                     AAAAVTGHFADIRNIK"
     gene            complement(7632..8723)
                     /gene="leuB"
                     /locus_tag="PN977_002034"
     CDS             complement(7632..8723)
                     /gene="leuB"
                     /locus_tag="PN977_002034"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HDI6042063.1"
                     /translation="MSKNYHIAVLPGDGIGPEVMTQALKVLDAVRNRFAMRITTSHYD
                     VGGAAIDNHGQPLPPATVEGCEQADAVLFGSVGGPKWEHLPPDQQPERGALLPLRKHF
                     KLFSNLRPAKLYQGLEAFCPLRADIAANGFDILCVRELTGGIYFGQPKGREGSGQYEK
                     AFDTEVYHRFEIERIARIAFESARKRRHKVTSIDKANVLQSSILWREIVNEIATEYPD
                     VELAHMYIDNATMQLIKDPSQFDVLLCSNLFGDILSDECAMITGSMGMLPSASLNEQG
                     FGLYEPAGGSAPDIAGKNIANPIAQILSLALLLRYSLDADDAASAIERAINRALEEGI
                     RTGDLARGAAAVSTNEMGDIIARYVVEGV"
     gene            complement(8723..10294)
                     /gene="leuA"
                     /locus_tag="PN977_002035"
     CDS             complement(8723..10294)
                     /gene="leuA"
                     /locus_tag="PN977_002035"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HDI6042064.1"
                     /translation="MSQQVIIFDTTLRDGEQALQASLSAKEKLQIALALERMGVDVME
                     VGFPVSSPGDFESVQTIARQVKNSRVCALARCVEKDIDVAAESLKVAEAFRIHTFIAT
                     SPMHIATKLRSTLDEVIERAIYMVKRARNYTDDVEFSCEDAGRTPIADLARVVEAAIN
                     AGATTINIPDTVGYTMPFEFAGIISGLYERVPNIDKAIISVHTHDDLGLAVGNSLAAV
                     HAGARQVEGAMNGIGERAGNCSLEEVIMAIKVRKDILNVHTAINHQEIWRTSQLVSQI
                     CNMPIPANKAIVGSGAFAHSSGIHQDGVLKNRENYEIMTPESIGLNQIQLNLTSRSGR
                     AAVKHRMDEMGYKESEYNLDNLYDAFLKLADKKGQVFDYDLEALAFIGKQQEEPEHFR
                     LDYFSVQSGSNDIATAAVKLACGEEVKAEAANGNGPVDAVYQAINRITDYNVELVKYS
                     LTAKGHGKDALGQVDIVANYNGRRFHGVGLATDIVESSAKAMVHVLNNIWRAAEVEKE
                     LQRKAQHNENNKETV"
     gene            complement(10387..10473)
                     /gene="leuL"
                     /locus_tag="PN977_002036"
     CDS             complement(10387..10473)
                     /gene="leuL"
                     /locus_tag="PN977_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leu operon leader peptide"
                     /protein_id="HDI6042065.1"
                     /translation="MTHIVRFIGLLLLNASSLRGRRVSGIQH"
     gene            11132..12076
                     /gene="leuO"
                     /locus_tag="PN977_002037"
     CDS             11132..12076
                     /gene="leuO"
                     /locus_tag="PN977_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LeuO"
                     /protein_id="HDI6042066.1"
                     /translation="MPEVQTDQPETAELSKPQLRMVDLNLLTVFDAVMQEQNITRAAH
                     VLGMSQPAVSNAVARLKVMFNDELFVRYGRGIQPTARAFQLFGSVRQALQLVQNELPG
                     SGFEPASSERVFHLCVCSPLDSILTSQIYNHIEQTAPNIHVMFKSSLNQNTEHQLRYQ
                     ETEFVISYEDFHRPEFTSVPLFKDEMVLVASKNHPTIKGPLLKHDVYNEQHAAVSLDR
                     FASFSQPWYDTVDKQASIAYQGMAMMSVLSVVSQTHLVAIAPRWLAEEFAESLELQVL
                     PLPLKQNSRTCYLSWHEAAGRDKGHQWMEEQLVSICKR"
     gene            complement(12231..12275)
                     /locus_tag="PN977_002038"
     CDS             complement(12231..12275)
                     /locus_tag="PN977_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6042067.1"
                     /translation="MKRNDKILHYRGLN"
     gene            12394..14118
                     /gene="ilvI"
                     /locus_tag="PN977_002039"
     CDS             12394..14118
                     /gene="ilvI"
                     /locus_tag="PN977_002039"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020079613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 3 large subunit"
                     /protein_id="HDI6042068.1"
                     /translation="MEMLSGAEMVVRSLIDQGVKQVFGYPGGAVLDIYDALHTVGGID
                     HVLVRHEQAAVHMADGLARATGEVGVVLVTSGPGATNAITGIATAYMDSIPLVVLSGQ
                     VATSLIGYDAFQECDMVGISRPVVKHSFLVKQTEDIPQVLKKAFWLAASGRPGPVVVD
                     LPKDILNPANKLPYVWPESVSMRSYNPTTSGHKGQIKRALQTLVAAKKPVVYVGGGAI
                     TAGCHQQLKETVEALNLPVVSSLMGLGAFPATHRQALGMLGMHGTYEANMTMHNADVI
                     FAVGVRFDDRTTNNLAKYCPNATVLHIDIDPTSISKTVTADIPIVGDARQVLEQMFEL
                     LSQESVHQPLDEIRDWWQQIEQWRARQCLKYDSHSEKIKPQAVIETLWRLTKGDAYVT
                     SDVGQHQMFAALYYPFDKPRRWINSGGLGTMGFGLPAALGVKMALPEETVVCVTGDGS
                     IQMNIQELSTALQYELPVLVVNLNNRYLGMVKQWQDMIYSGRHSQSYMQSLPDFVRLA
                     EAYGHVGIQISHPQELESKLSEALEQVRNNRLVFVDVTVDGSEHVYPMQIRGGGMDEM
                     WLSKTERT"
     gene            14121..14612
                     /gene="ilvN"
                     /locus_tag="PN977_002040"
     CDS             14121..14612
                     /gene="ilvN"
                     /locus_tag="PN977_002040"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HDI6042069.1"
                     /translation="MRRILSVLLENESGALSRVIGLFSQRGYNIESLTVAPTDDPTLS
                     RMTIQTVGDEKVLEQIEKQLHKLVDVLRVSELGQGAHVEREIMLVKIQASGYGRDEVK
                     RNTEIFRGQIIDVTPSLYTVQLAGTSDKLDAFLASIRDVAKIVEVARSGVVGLSRGEK
                     IMR"
     gene            14624..14710
                     /locus_tag="PN977_002041"
     CDS             14624..14710
                     /locus_tag="PN977_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fruR/shl operon leader peptide"
                     /protein_id="HDI6042070.1"
                     /translation="MRNLQPNMSRWAFFAKSVGTWNKSSCRS"
     gene            14792..15796
                     /gene="cra"
                     /locus_tag="PN977_002042"
     CDS             14792..15796
                     /gene="cra"
                     /locus_tag="PN977_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite repressor/activator"
                     /protein_id="HDI6042071.1"
                     /translation="MKLDEIARLAGVSRTTASYVINGKAKQYRVSDKTVEKVMAVVRE
                     HNYHPNAVAAGLRAGRTRSIGLVIPDLENTSYTRIANYLERQARQRGYQLLIACSEDQ
                     PDNEMRCIEHLLQRQVDAIIVSTSLPPEHPFYQRWANDPFPIVALDRALDREHFTSVV
                     GADQDDAEMLAEELRKFPAETVLYLGALPELSVSFLREQGFRTAWKDDPREVHFLYAN
                     SYEREAAAQLFEKWLETHPMPQALFTTSFALLQGVMDVTLRRDGKLPSDLAIATFGDN
                     ELLDFLQCPVLAVAQRHRDVAERVLEIVLASLDEPRKPKPGLTRIKRNLYRRGVLSRS
                     "
     gene            16398..16856
                     /gene="mraZ"
                     /locus_tag="PN977_002043"
     CDS             16398..16856
                     /gene="mraZ"
                     /locus_tag="PN977_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020325403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HDI6042072.1"
                     /translation="MFRGATLVNLDSKGRLSVPTRYREQLLENAAGQMVCTIDIHHPC
                     LLLYPLPEWEIIEQKLSRLSSMNPVERRVQRLLLGHASECQMDGAGRLLIAPVLRQHA
                     GLTKEVMLVGQFNKFELWDETTWHQQVKEDIDAEQLATGDLSERLQDLSL"
     gene            16858..17799
                     /gene="rsmH"
                     /locus_tag="PN977_002044"
     CDS             16858..17799
                     /gene="rsmH"
                     /locus_tag="PN977_002044"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HDI6042073.1"
                     /translation="MMENYKHTTVLLDEAVNGLNIRPDGIYIDGTFGRGGHSRLILSQ
                     LGEEGRLLAIDRDPQAIAVAKTIDDPRFSIIHGPFSALGEYVAERDLIGKIDGILLDL
                     GVSSPQLDDAERGFSFMRDGPLDMRMDPTRGQSAAEWLQTAEEADIAWVLKTYGEERF
                     AKRIARAIVERNREQPMTRTKELAEVVAAATPVKDKFKHPATRTFQAVRIWVNSELEE
                     IEQALKSSLNVLAPGGRLSIISFHSLEDRIVKRFMRENSRGPQVPAGLPMTEEQLKKL
                     GGRQLRALGKLMPGEEEVAENPRARSSVLRIAERTNA"
     gene            17796..18161
                     /gene="ftsL"
                     /locus_tag="PN977_002045"
     CDS             17796..18161
                     /gene="ftsL"
                     /locus_tag="PN977_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HDI6042074.1"
                     /translation="MISRVTEALSKVKGSMGSHERHALPGVIGDDLLRFGKLPLCLFI
                     CIILTAVTVVTTAHHTRLLTAQREQLVLERDALDIEWRNLILEENALGDHSRVERIAT
                     EKLQMQHVDPSQENIVVQK"
     gene            18177..19943
                     /gene="ftsI"
                     /locus_tag="PN977_002046"
     CDS             18177..19943
                     /gene="ftsI"
                     /locus_tag="PN977_002046"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000642204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase FtsI"
                     /protein_id="HDI6042075.1"
                     /translation="MKAAAKTQKPKRQEEHANFISWRFALLCGCILLALAFLLGRVAW
                     LQVISPDMLVKEGDMRSLRVQQVSTSRGMITDRSGRPLAVSVPVKAIWADPKEVHDAG
                     GISVGDRWKALANALNIPLDQLSARINANPKGRFIYLARQVNPDMAEYIKKLKLPGIH
                     LREESRRYYPSGEVTAHLIGFTNVDSQGIEGVEKSFDKWLTGQPGERIVRKDRYGRVI
                     EDISSTDSQAAHNLALSIDERLQALVYRELNNAVAFNKAESGSAVLVDVNTGEVLAMA
                     NSPSYNPNNLSGTPKEAMRNRTITDVFEPGSTVKPMVVMTALQRGVVRENSVLNTVPY
                     RINGHEIKDVARYSELTLTGVLQKSSNVGVSKLALAMPSSALVDTYSRFGLGKATNLG
                     LVGERSGLYPQKQRWSDIERATFSFGYGLMVTPLQLARVYATIGSYGIYRPLSITKVD
                     PPVPGERVFPESIVRTVVHMMESVALPGGGGVKAAIKGYRIAIKTGTAKKVGPDGRYI
                     NKYIAYTAGVAPASQPRFALVVVINDPQAGKYYGGAVSAPVFGAIMGGVLRTMNIEPD
                     ALTTGDKNEFVINQGEGTGGRS"
     gene            19930..21417
                     /gene="murE"
                     /locus_tag="PN977_002047"
     CDS             19930..21417
                     /gene="murE"
                     /locus_tag="PN977_002047"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HDI6042076.1"
                     /translation="MADRNLRDLLAPWVPDAPSRALREMTLDSRVAAAGDLFVAVVGH
                     QADGRRYIPQAIAQGVAAIIAEAKDEATDGEIREMHGVPVIYLSQLNERLSALAGRFY
                     HEPSDNLRLVGVTGTNGKTTTTQLLAQWSQLLGETSAVMGTVGNGLLGKVIPTENTTG
                     SAVDVQHELAGLVDQGATFCAMEVSSHGLVQHRVAALKFAASVFTNLSRDHLDYHGDM
                     EHYEAAKWLLYSEHRCGQAIINADDEVGRRWLAKLPDAVAVSMEDHINLNCHGRWLKA
                     TEVNYHDSGATIRFSSSWGDGEIESHLMGAFNVSNLLLALATLLALGYPLADLLKTAA
                     RLQPVCGRMEVFTAPGKPTVVVDYAHTPDALEKALQAARLHCAGKLWCVFGCGGDRDK
                     GKRPLMGAIAEEFADVAVVTDDNPRTEEPRAIINDILAGMLDAGHAKVMEGRAEAVTC
                     AVMQAKENDVVLVAGKGHEDYQIVGNQRLDYSDRVTVARLLGVIA"
     gene            21414..22772
                     /gene="murF"
                     /locus_tag="PN977_002048"
     CDS             21414..22772
                     /gene="murF"
                     /locus_tag="PN977_002048"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HDI6042077.1"
                     /translation="MISVTLSQLTDILNGELQGADITLDAVTTDTRKLTPGCLFVALK
                     GERFDAHDFADQAKAGGAGALLVSRPLDIDLPQLIVKDTRLAFGELAAWVRQQVPARV
                     VALTGSSGKTSVKEMTAAILSQCGNTLYTAGNLNNDIGVPMTLLRLTPEYDYAVIELG
                     ANHQGEIAWTVSLTRPEAALVNNLAAAHLEGFGSLAGVAKAKGEIFSGLPENGIAIMN
                     ADNNDWLNWQSVIGSRKVWRFSPNAANSDFTATNIHVTSHGTEFTLQTPTGSVDVLLP
                     LPGRHNIANALAAAALSMAVGATLDAIKAGLANLKAVPGRLFPIQLVENQLLLDDSYN
                     ANVGSMTAAVQVLAEMPGYRVLVVGDMAELGAESEACHIQVGEAAKAAGIDRVLSVGK
                     QSHAISTASGVGEHFSDKTALIARLKSLIAEQQVITILVKGSRSAAMEEVVRALQENG
                     TC"
     gene            22766..23848
                     /gene="mraY"
                     /locus_tag="PN977_002049"
     CDS             22766..23848
                     /gene="mraY"
                     /locus_tag="PN977_002049"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HDI6042078.1"
                     /translation="MLVWLAEHLVKYYSGFNVFSYLTFRAIVSLLTALFISLWMGPRM
                     IAHLQKLSFGQVVRNDGPESHFSKRGTPTMGGIMILTAIVISVLLWAYPSNPYVWCVL
                     VVLVGYGIIGFVDDYRKVVRKDTKGLIARWKYFWMSVIALGVAFALYLAGKDTPATQL
                     VVPFFKDVMPQLGLFYILLAYFVIVGTGNAVNLTDGLDGLAIMPTVFVAGGFALVAWA
                     TGNMNFASYLHIPYLRHAGELVIVCTAIVGAGLGFLWFNTYPAQVFMGDVGSLALGGA
                     LGIIAVLLRQEFLLVIMGGVFVVETLSVILQVGSFKLRGQRIFRMAPIHHHYELKGWP
                     EPRVIVRFWIISLMLVLIGLATLKVR"
     gene            23851..25167
                     /gene="murD"
                     /locus_tag="PN977_002050"
     CDS             23851..25167
                     /gene="murD"
                     /locus_tag="PN977_002050"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HDI6042079.1"
                     /translation="MADYQGKNVVIIGLGLTGLSCVDFFLARGVTPRVMDTRMTPPGL
                     DKLPEAVERHTGGLNDEWLMAADLIVASPGIALAHPSLSAAADAGIEIVGDIELFCRE
                     AQAPIVAITGSNGKSTVTTLVGEMAKAAGVNVGVGGNIGLPALMLLDDECELYVLELS
                     SFQLETTSSLQAVAATILNVTEDHMDRYPFGLQQYRAAKLRIYENAKICVVNADDALT
                     MPIRGADERCVSFGVNMGDYHLNHQQGETWLRVKGEKVLNVKEMKLSGQHNYTNALAA
                     LALADAAGLPRASSLKALTTFTGLPHRFEVVLEHNGVRWINDSKATNVGSTEAALNGL
                     HVDGTLHLLLGGDGKSADFSPLARYLNGDNVRLYCFGRDGAQLAALRPEVAEQTETME
                     QAMRLLAPRVQPGDMVLLSPACASLDQFKNFEQRGNEFARLAKELG"
     gene            25167..26411
                     /gene="ftsW"
                     /locus_tag="PN977_002051"
     CDS             25167..26411
                     /gene="ftsW"
                     /locus_tag="PN977_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsW"
                     /protein_id="HDI6042080.1"
                     /translation="MRLSLPRLKMPRLPGFSILVWISTALKGWVMGSREKDTDSLIMY
                     DRTLLWLTFGLAAIGFIMVTSASMPIGQRLTNDPFFFAKRDGVYLILAFILAIITLRL
                     PMEFWQRYSATMLLGSIILLMIVLVVGSSVKGASRWIDLGLLRIQPAELTKLSLFCYI
                     ANYLVRKGDEVRNNLRGFLKPMGVILVLAVLLLAQPDLGTVVVLFVTTLAMLFLAGAK
                     LWQFIAIIGMGISAVVLLILAEPYRIRRVTAFWNPWEDPFGSGYQLTQSLMAFGRGEL
                     WGQGLGNSVQKLEYLPEAHTDFIFAIIGEELGYVGVVLALLMVFFVAFRAMSIGRKAL
                     EIDHRFSGFLACSIGIWFSFQALVNVGAAAGMLPTKGLTLPLISYGGSSLLIMSTAIM
                     MLLRIDYETRLEKAQAFVRGSR"
     gene            26408..27475
                     /gene="murG"
                     /locus_tag="PN977_002052"
     CDS             26408..27475
                     /gene="murG"
                     /locus_tag="PN977_002052"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HDI6042081.1"
                     /translation="MSGQGKRLMVMAGGTGGHVFPGLAVAHHLMAQGWQVRWLGTADR
                     MEADLVPKHGIEIDFIRISGLRGKGIKALIAAPLRIFNAWRQARAIMKAYKPDVVLGM
                     GGYVSGPGGLAAWSLGIPVVLHEQNGIAGLTNKWLAKIATKVMQAFPGAFPNAEVVGN
                     PVRTDVLALPLPQQRLAGREGPVRVLVVGGSQGARILNQTMPQVAAKLGDSVTIWHQS
                     GKGSQQSVEQAYAEAGQPQHKVTEFIDDMAAAYAWADVVVCRSGALTVSEIAAAGLPA
                     LFVPFQHKDRQQYWNALPLEKAGAAKIIEQPQLSVDAVANTLAGWSRETLLTMAERAR
                     AASIPDATERVANEVSRAARA"
     gene            27529..29004
                     /gene="murC"
                     /locus_tag="PN977_002053"
     CDS             27529..29004
                     /gene="murC"
                     /locus_tag="PN977_002053"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HDI6042082.1"
                     /translation="MNTQQLAKLRSIVPEMRRVRHIHFVGIGGAGMGGIAEVLANEGY
                     QISGSDLAPNPVTQQLMNLGATIYFNHRPENVRDASVVVVSSAISADNPEIVAAHEAR
                     IPVIRRAEMLAELMRFRHGIAIAGTHGKTTTTAMVSSIYAEAGLDPTFVNGGLVKAAG
                     VHARLGHGRYLIAEADESDASFLHLQPMVAIVTNIEADHMDTYQGDFENLKQTFINFL
                     HNLPFYGRAVMCVDDPVIRELLPRVGRQTTTYGFSEDADVRVEDYQQIGPQGHFTLLR
                     QDKEPMRVTLNAPGRHNALNAAAAVAVATEEGIDDEAILRALESFQGTGRRFDFLGEF
                     PLEPVNGKSGTAMLVDDYGHHPTEVDATIKAARAGWPDKNLVMLFQPHRFTRTRDLYD
                     DFANVLTQVDTLLMLEVYPAGEAPIPGADSRSLCRTIRGRGKIDPILVPDPAQVAEML
                     APVLTGNDLILVQGAGNIGKIARSLAEIKLKPQTPEEEQHD"
     gene            28997..29917
                     /gene="ddlB"
                     /locus_tag="PN977_002054"
     CDS             28997..29917
                     /gene="ddlB"
                     /locus_tag="PN977_002054"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HDI6042083.1"
                     /translation="MTDKIAVLLGGTSAEREVSLNSGAAVLAGLREGGIDAYPVDPKE
                     VDVTQLKSMGFQKVFIALHGRGGEDGTLQGMLELMGLPYTGSGVMASALSMDKLRSKL
                     LWQGAGLPVAPWVALTRAEFEKGLNDQQLAEISSLGLPVIVKPSREGSSVGMSKVVAE
                     NALQDALRLAFQHDEEVLIEKWLSGPEFTVAILGEEILPSIRIQPAGTFYDYEAKYLS
                     DETQYFCPAGLEALQEANLQALVLKAWTTLGCKGWGRIDVMLDSDGQFYLLEANTSPG
                     MTSHSLVPMAARQAGMSFSQLVVRILELAD"
     gene            29919..30749
                     /gene="ftsQ"
                     /locus_tag="PN977_002055"
     CDS             29919..30749
                     /gene="ftsQ"
                     /locus_tag="PN977_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ"
                     /protein_id="HDI6042084.1"
                     /translation="MSQAALNTRNSEEEVSSRRNNGTRLAGILFLLTVLTTVLVSGWV
                     VLGWMEDAQRLPLSKLVLTGERHYTRNDDIRQSILALGEPGTFMTQDVNIIQTQIEQR
                     LPWIKQVSVRKQWPDELKIHLVEYVPIARWNDQHMVDAEGNTFSVPPDRTSKQVLPML
                     YGPEGSANEVLQGYREMGQMLAKDRFTLKEAAMTARRSWQLTLNNDIKLNLGRGDTMK
                     RLARFVELYPVLQQQAQTDGKRISYVDLRYDSGAAVGWAPLPPEESTQQQNQAQAEQQ
                     "
     gene            30746..32008
                     /gene="ftsA"
                     /locus_tag="PN977_002056"
     CDS             30746..32008
                     /gene="ftsA"
                     /locus_tag="PN977_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HDI6042085.1"
                     /translation="MIKATDRKLVVGLEIGTAKVAALVGEVLPDGMVNIIGVGSCPSR
                     GMDKGGVNDLESVVKCVQRAIDQAELMADCQISSVYLALSGKHISCQNEIGMVPISEE
                     EVTQEDVENVVHTAKSVRVRDEHRVLHVIPQEYAIDYQEGIKNPVGLSGVRMQAKVHL
                     ITCHNDMAKNIVKAVERCGLKVDQLIFAGLASSYSVLTEDERELGVCVVDIGGGTMDI
                     AVYTGGALRHTKVIPYAGNVVTSDIAYAFGTPPSDAEAIKVRHGCALGSIVGKDESVE
                     VPSVGGRPPRSLQRQTLAEVIEPRYTELLNLVNEEILQLQEKLRQQGVKHHLAAGIVL
                     TGGAAQIEGLAACAQRVFHTQVRIGAPLNITGLTDYAQEPYYSTAVGLLHYGKESHLN
                     GEAEVEKRVTASVGSWIKRLNSWLRKEF"
     gene            32070..33221
                     /gene="ftsZ"
                     /locus_tag="PN977_002057"
     CDS             32070..33221
                     /gene="ftsZ"
                     /locus_tag="PN977_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HDI6042086.1"
                     /translation="MFEPMELTNDAVIKVIGVGGGGGNAVEHMVRERIEGVEFFAVNT
                     DAQALRKTAVGQTIQIGSGITKGLGAGANPEVGRNAADEDRDALRAALEGADMVFIAA
                     GMGGGTGTGAAPVVAEVAKDLGILTVAVVTKPFNFEGKKRMAFAEQGITELSKHVDSL
                     ITIPNDKLLKVLGRGISLLDAFGAANDVLKGAVQGIAELITRPGLMNVDFADVRTVMS
                     EMGYAMMGSGVASGEDRAEEAAEMAISSPLLEDIDLSGARGVLVNITAGFDLRLDEFE
                     TVGNTIRAFASDNATVVIGTSLDPDMNDELRVTVVATGIGMDKRPEITLVTNKQVQQP
                     VMDRYQQHGMAPLTQEQKPVAKVVNDNAPQTAKEPDYLDIPAFLRKQAD"
     gene            33322..>33982
                     /gene="lpxC"
                     /locus_tag="PN977_002058"
     CDS             33322..>33982
                     /gene="lpxC"
                     /locus_tag="PN977_002058"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HDI6042087.1"
                     /translation="MIKQRTLKRIVQATGVGLHTGKKVTLTLRPAPANTGVIYRRTDL
                     NPPVDFPADAKSVRDTMLCTCLVNEHDVRISTVEHLNAALAGLGIDNIVIEVNAPEIP
                     IMDGSAAPFVYLLLDAGIDELNCAKKFVRIKETVRVEDGDKWAEFKPYNGFSLDFTID
                     FNHPAIDSSNQRYAMNFSADAFMRQISRARTFGFMRDIEYLQSRGLCLGGSFDCAIVV
                     DD"
BASE COUNT         7930 a         8375 c         9483 g         8194 t
ORIGIN      
        1 caatttgctg gtagagataa aacggcaggg tacggaaatc atcgttaccg aacaacgcca
       61 ccacgccaaa atcaccaatc gacagcacac aagcaaaggc cagtgcctgc gccagcggac
      121 gtttcagggc gcgcagctcc accactttta agcgtgacca gccttcaatg cccagcgact
      181 gacataacat gttgtagcga gcggtgatat cgcgcatcgg gttttccagc actttcagcg
      241 cataagggat cgccattaac gcattggtga aaatcacaat gccgtcagca gattgtggca
      301 ggccgatagt gttgttgagc agtaaaaaga agcccgttgc cagcacaatc cccggcatgg
      361 cgaggatcaa catgccgctc atctccagcg cctggccggc cagcattttc tgccgcgccc
      421 gcagttcgcg actgctccat agcagcatca tggtcagcac tacgcacaat acgcctgccg
      481 ccagcgcaat acgcaacgag gtccacagcg cctgccacag caccggttgt gccagcactt
      541 ccggcaactg gcgatttacc ccatcgacga tcaccgccag taacggtggc agcaatagca
      601 gcagcgccag cacaattaac acagtgtcgc aaatgcggct atgcagacga tcgtccgggt
      661 cgcgccagcc ttgcagcagc gtggtgcctg gcgcaatggc cttactcaat cgctgactca
      721 gcagcaccag cccaaggcaa cacaccatct ggatcagcgc aagcatcgcc gcgcgggcag
      781 gatcgtagtc gtaactcagc gcctgataaa tagccagctc gatagtggtc gcctgcggac
      841 cgccccccag cgatagcacg gtggcgaagc tggcgaaaca gagcataaag ataagcgcag
      901 caactggcgg gatttgtcga cgtaaccacg gccattcgac aaagcggaaa aaatgccagc
      961 cgcgcatccc aagttgtgcg gcaagttgac gttgttcgcc gggaatgttt tccagtgcct
     1021 gaagtaataa acggctcgcc atcggcagat taaaaaatac gtgcgccagc aaaatacctt
     1081 gcaggccgta gggcgaaaag gtccactcca gaccaagtga ttgccagagc gaggccagcc
     1141 agccctggcg accatagacg ctaagaatgc cgaaaacagc gaccagcacc gggaggatca
     1201 aggtcattgc gcacagacgc aacagcgcca gccgacctgg aaagcgcctg cgatagaggg
     1261 cgcgggcgag gaatatcgcc ggaacgacag agagcagcgc cgagagaaac gcctgccaga
     1321 aggagaagcg caccacatgc cacagatagc tgtcctgcca gactgccacc cagtcaccct
     1381 gcggcgcgtt ccaccacagg gcgagaaacg ccgccagcgc aaccgccacc acaagcgtag
     1441 cggcgcttac acctggaatt aaccagccgg gaattaacgg ctgacggcgc gttgccattc
     1501 gctaatccat gcctgacgtt gtgccgccac ttcggctggc gtgaactcca gcgtggttgc
     1561 cggtttggtc aattgttcaa acccggcagg cagcgtgaca tttgccaccg gatacatcca
     1621 gttgccggtt gggatcgcat tctggaaagc cggagaaacc ataaactgga gaaatttttg
     1681 cgccagctct ggctgcttgc tggcagcggt gcgggcggcg acttccacct gcagatagtg
     1741 accttcgctg aagttcgcgg cggcgtagtt atctttcttc tcttcgagaa tgtgataagc
     1801 cggagaggtg gtgtaactca gaaccagatc gctttcgcct tttaaaaaca ggccgtaggc
     1861 ttcgctccag cctttggtga ccgtgacagt tttcttcgcc agtttctgcc aggcttgcgg
     1921 ggcgttatcg ccatagactt tttgcatcca tagcaacaga cccagccccg gtgtactggt
     1981 acgtggatct tcataaatca cccgccagtt ttgatcgctt tcaaccagct ctttcaggct
     2041 ttgcggcgga tttttcagtt tgttcttgtc atagacgaag gcgaaatagc cgtaatcaaa
     2101 cgggacgaaa gtgtcattat tccagccgcc gggaacgtta acggcatccg cagccacacc
     2161 gcttttagca aacagcccgg ttttgctggc ggcatctaac agattgttat ccagccccag
     2221 caccacatcg gctttgctgt ttttgccttc catccgtaga cggttgagaa acgaaacgcc
     2281 atcttccaga gccaccagtt tcagttcgca attacagtcg gcttcaaagg cttttttaac
     2341 caccggacca ggcccccagt cggcggcgaa ggaatcgtag gtgtagacgg tcagaacggg
     2401 tttagcgaaa acgggcgctg tgcacagcaa cagcagggga agatattttt ttaacacttt
     2461 gcacctcaaa gaagagtggc aaaggacttg agaaggagcc tcaaatccct tcgccggcgt
     2521 tatccggatc aggttcgacg ggtattttct cagcgcacgc ggcagcgtgg caccccgttg
     2581 agaacggcgt tagtgtagtg attttgttat caaccagcaa tccatagaga aggcgtatta
     2641 aaaaagccaa tattcgttca gctatcaagg acgtatttgc atagctggga agctttgaac
     2701 cgaaagaatc ccttagcaca gtcaaaatcg gtaatgcaac ggcttattgc gtgaatgagc
     2761 gatttttgac agtattctta ttcaggacgc ttattctgag tttttaactc agagtggagc
     2821 tatcagcatg aaagtaagaa acccagaaca gattagtatc tctgctagta ataccactaa
     2881 agatccaggc cttactcatt ctcagattat gaggatgttt aacctggtca aaaagaccga
     2941 gaatatgaat ctctttgaag cgctatggga gacgttacgc aatctcttcc ggtcagataa
     3001 acattcccag gctgcggcaa ggcaaattct gaaggatgca ttttatttcc ataatagtga
     3061 tgactactca aaatacttta ccggggctgt cgacggggat gcgcgtgata agtttaccca
     3121 ctggctgata aattttaata aactcaaaga acatgcaata gatccggaaa atatggccgc
     3181 gaaggcctca ctctcgccaa aagatacgtt aagagtcagt ttttttattg gcgatgaggc
     3241 gatatttact ctggagttgc agatgaaaga gaacaccagg accgggtgca ttgatctgag
     3301 taatgcttac tttaacgggg ttgtcatttg tggtattgac cgtctcgagg ttgacctgag
     3361 caatgctgaa atgaacaata gcagatggta tgactgacct gtgcgaggtt aacctgaaaa
     3421 agctgaccgt tccggccaga acaaggccag actgcgccgt tattgattaa ctcagaatat
     3481 gaaagagggg ctggagtgcc ccttctttaa tcttatttgc gacgctaatc agacgatgca
     3541 taaaacagag aaaatataaa agtaatgtat tttattcatt ccgtctgaat ataaaaaacg
     3601 cgctcatgac cgccaaaagg cgcaagctac tctggatccg gcggcgcaaa ccacgctgat
     3661 ttaaaatcga accagccgag ggtgttcata cgtagaccgc gcatactgcg ttgcccctga
     3721 atgatcagcc agtggtggat aagcggcacc attgctttgc tggcgaccag ttgctggcac
     3781 cagttcgcca ggttcatttc gccattgcgc cagcgggcgg cgtcggcttg ccagtcgatc
     3841 ggaagacagt gttggagcag cggcacctcg cacaagtgcg cgaacagcga aaaatccagc
     3901 ggcagcgtaa agttggcgct gttaagccag atatcgctct cgatctctcc ttcatgccac
     3961 tgatcgtagc tgatctcttt gatttccagc gtgacctggt gacttgccag aatctgctgc
     4021 atgatcccgg caatcactcg atgctcaata tgatcctgat aaaaggtcag ggtgaggctt
     4081 tccagcccgg cgggtttttc gcaatgtgtc ggacgagcat ggtgccaacg ggggagcagt
     4141 ccataagccg ggaaccacag ttgctggtac tgttcctcag cgaaatagac cagattagtt
     4201 ggagaaagca catagcttac ccagtccctg acttgctgat tcgccccgcg atgggtgcgg
     4261 ctgtcgaaca gtaaatagta gcaaccttcc tccaggcggc tttcaatctc tttttcctcg
     4321 ccctgtggcc cttttagcat cagccctccg gctggctcgt cggcaatttc cggcagaacc
     4381 cagacgttaa cttcgtcgat taatgcccgg taaccgaaga agtcatcgaa tgcctgaatt
     4441 ttcagttgat tggtgctgtt gcggatcacc gcatacggac cggtgccgat gggatggctg
     4501 gcaaagttac tgagggtttc ccattcgcgc ggcaggatca tcgccggaac ttgccccagc
     4561 agtaacggca accagcgatc cggctgcgtg aggtggatat ccagcgtcca gggcgtcggc
     4621 gatacaattt cagcaatatg cgaatagagc ggcagcgtat tgatccgttt taaagaggcg
     4681 atcacatcgt ccatttccag ttcacgacca tgatgaaaat ggactcctgg acgcaaaaag
     4741 aaacgccagt gaagcggtga tatttgctgc cagtggtggg cgatgtctgc ttccagttcc
     4801 ccattttcct catttatgcg cgttagcgaa ctgaagattt gccgggcgat atgggtttcg
     4861 gaacggcgta atgcgctgcc aggtagcaga ttacgcaacg gacgatagta gagcacacgc
     4921 aggatgtgtc gcccctggcg gaagctgcgg cccagatgag aaaccagcat ttgccgcaca
     4981 gtcgctttgt cgccaaccag ttgcaccagt tgatcgatac gatcctgctc cagcaggtct
     5041 tccgcccgct gttgctgaag cgccagcccg gtatagagga atgtcagacg cgagcgttta
     5101 ccgcgcccga cttccgcttc ccacgtcagc cagccgcgat cctgcatggt gttgagcagg
     5161 gtgcgcatat gacgacgcga gcagctcaat aacgctgcca gttcgttgag cgttgtgtcc
     5221 tgcgatttac cctcgcagca ttgccacagg cggatgaact gttgttgcag acgagcagat
     5281 ggcataaaag gggaactcct gcgcaaaagt cagcaatttt attttcccta tattaagtca
     5341 ataattccta acgatgaagc aaggaggtgc cccatgcgtc agttttatca gcactatttt
     5401 accgcgacag cgaagttgtg ctggttgcgt tggttaagcg tcccacaacg attaactatg
     5461 cttgaagaac tgatgcagtg ggaggggagt cattctgact actgatttgc ggacatcatg
     5521 tgtgactgag tattggtgct aatcgccacg ccagcagtga ttatctgctg gcttttttct
     5581 ttttggcgat tgagtgccgg acggtgtaca aactaacgca taaacgccgg ttgcttctct
     5641 tcataagacg caatggcgtc gtcgtgctgc agagtcaggc cgatgctgtc cagaccgttc
     5701 atcatgcagt ggcggcggaa tgcatcgatg gtaaaacgat aggttttctc acctgccttc
     5761 acctcttgcg cttccaggtc cacgtcgaaa tggatccccg gattggcttt cactagcgca
     5821 aacagctcat ccacttctgc atcgcttaat ttcaccggca gcagctggtt gttaaagctg
     5881 ttgccgtaga agatgtcagc aaaactcggt gcaatcacca ctttaaaacc gtaatcggtc
     5941 aatgcccacg gcgcgtgctc acgcgaggaa ccgcagccga agttttctcg tgccagcaaa
     6001 atggaagcgc cctgatactg cgggaagttc agcacgaagt ccgggtttgg ctgttggcct
     6061 ttttcatcca gaaaacgcca gtcgttaaac agatgcgcgc caaaacccgt acgggtcact
     6121 ttctgcaaaa actgtttcgg gatgattgca tcggtatcga cattggcggc atccagtgga
     6181 accaccaggc ctgtatgttt gataaatttc tctgccatgg tgtgctcctt atttaatgtt
     6241 gcgaatgtcg gcaaaatgtc cggtcacagc cgcggcggca gccatagccg ggctgaccag
     6301 atgcgtgcgc ccgccgcgcc cctggcggcc ttcaaagtta cggttactgg tggaggcaca
     6361 acgttcgccc ggattcagac ggtcgttatt catcgccaga cacattgagc agccaggcaa
     6421 gcgccattca aaaccggctt caataaagat tttatccaga ccttccgctt ccgcctgggc
     6481 ttttaccggg ccagagccgg gaaccaccag tgcctgcacg cctggcgcga cttttcgccc
     6541 tttggcgatt tccgctgccg cgcgtaaatc ttcaatgcgc gagttggtac aggaaccgat
     6601 aaacacttta tcgatagcca cttcggtcag cggaataccc ggtttcagcc ccatataggc
     6661 cagcgctttt tctgccgacg cgcgttcaac cggatcggca aacgaagctg gatcgggaat
     6721 attgtcgttc acggaaatca cctggcctgg attggtgccc caggtgacct gcggcgaaat
     6781 ctcttctgct tgcagagtga caacggtatc gaaagttgcg ccttcgtcgg tttgcagggt
     6841 tttccagtag gcgacggcgt cgtcgaaatc tttgcctttc ggcgcatgca gacggccttt
     6901 gacatagtta aaggtagttt cgtccggtgc aaccagaccg gctttcgcgc ccatttcgat
     6961 tgccatattg cacagggtca tacgaccttc catgcttaaa tcacggattg cttcgccgca
     7021 aaactccacc acatgcccgg tgccgcctgc gctaccggtt ttaccgataa tcgccagcac
     7081 gatatctttc gcggtaatgc ccggcgcggc tttgccctgg acttcaattt tcatggtctt
     7141 cgcacgaccc tgtttcaggg tttgcgttgc cagtacgtgt tcaacttcgg aagtgccgat
     7201 accaaaggcc agtgcgccaa acgcgccgtg ggtggcggta tgtgagtcgc cgcagacaat
     7261 ggtcatcccc ggcaatgtga cgccctgttc cggccccatt acgtggacga tcccctgata
     7321 cgggtgattc aggtcataca gctcgacgcc aaattctttg cagtttttga tcagctcctg
     7381 catctggatg cgcgccattt caccgcaggc attaatgtct ttggtctgag tagagacgtt
     7441 gtgatccatg gtggcgaagg ttttgcccgg ctgacgtacc gggcgaccgt gggcgcgcag
     7501 accatcgaac gcctgcggtg aggtcacttc atgcaccagg tggcggtcga tatataacag
     7561 cggggtttcg ttttcggctt cgtacactac gtgagcgtcg aacaattttt cgtataacgt
     7621 tttagccatg attacacccc ttctaccaca tagcgggcaa tgatatcgcc catttcattg
     7681 gtactaacgg cggcagcgcc acgggctaaa tccccggtgc gaatgccttc ttctaatgcg
     7741 cggttaatgg cgcgttcaat ggcggaagcc gcatcatcgg catccaggct gtaacgcagc
     7801 agcagcgcca gcgacagaat ttgtgcaatc gggttggcga tatttttacc tgcgatatcg
     7861 ggtgccgagc cgcccgccgg ttcatacaga ccaaaacctt gctcgttcag gctggcggaa
     7921 ggcaacatcc ccattgagcc agtgatcatt gcgcactcat cagacagaat gtcgccaaac
     7981 aggttggagc acagcaagac gtcaaactgt gatggatctt taatcagctg catggtggcg
     8041 ttgtcgatgt acatatgcgc cagttcgaca tccgggtatt ccgtggcgat ctcgttaacg
     8101 atctcccgcc ataaaataga ggattgcagc acgttggctt tgtcgattga ggtgactttg
     8161 tggcgacgct tgcgggcaga ttcaaatgcg atgcgggcaa tgcgctcgat ctcaaaacgg
     8221 tggtacacct cggtatcaaa cgctttttca tattgtccgc taccttcgcg gccttttggc
     8281 tgaccgaaat agataccgcc ggtcagttcg cgcacacaca ggatgtcaaa gccgtttgcg
     8341 gcaatgtcag cacgcagcgg acagaatgct tccagcccct gatacagttt tgccggacgc
     8401 agattgctga ataatttgaa gtgcttacgc aaaggcagca gtgcgccgcg ttctggttgc
     8461 tggtctggcg gcaaatgttc ccacttcgga ccacctaccg agccaaacag cacggcatcg
     8521 gcttgctcac aaccttcaac cgtcgcaggc ggcagcggct gcccgtggtt atcaatggct
     8581 gcgccgccta catcgtaatg gctggtggtg atacgcatcg caaagcggtt gcgcacggca
     8641 tccagcactt tcagcgcctg ggtcatcact tccggaccaa taccgtcccc cggcaatacg
     8701 gcaatatggt aattcttcga catcacacgg tttccttgtt gttttcgttg tgttgagctt
     8761 tgcgttgcaa ctctttttcg acttctgcgg cacgccagat attgttcagt acgtgcacca
     8821 tggctttggc ggaggactcg acaatatcgg tggccaggcc gacgccgtgg aagcggcgac
     8881 cgttgtagtt ggcgacaata tccacctgac ccagcgcatc tttaccgtga cctttggcgg
     8941 tcaggctgta tttcaccagt tcgacgttat agtcagtgat gcggtttatc gcctggtaga
     9001 cggcatcgac cggaccgtta ccgttggcgg cttctgcttt gacttcttcg ccacaggcca
     9061 gtttgacggc ggcagtggca atatcgttag aacctgactg cacgctgaag taatccagac
     9121 ggaaatgctc cggctcttct tgctgcttac cgatgaaggc cagcgcctcc agatcgtaat
     9181 caaacacctg gccttttttg tccgccagct tcaggaaagc gtcgtacagg ttgtctaaat
     9241 tatattcact ttctttatat cccatctcat ccatgcgatg tttcaccgcc gcacgccccg
     9301 aacgagaggt cagattcagc tggatttggt tcagaccaat agattctggt gtcatgattt
     9361 cgtagttttc acggtttttc agtacaccat cctggtggat accggaggag tgtgcgaatg
     9421 cgccgctgcc aacaatggct ttgtttgccg ggatcggcat attacaaatc tggctaacta
     9481 actggctggt gcgccatatc tcctggtgat taatggcggt atgaacgttg agaatatcct
     9541 tacgaacttt gatcgccatg atcacttctt ccagcgaaca gttaccggct cgctcgccga
     9601 tcccattcat tgcaccttcc acctggcgcg ctccggcatg taccgccgcc agtgagttgc
     9661 caactgccag gcctaaatcg tcgtgggtat gtacggagat aatggctttg tcgatgttag
     9721 gcacgcgttc atacagcccg ctgatgattc cggcgaactc aaacggcatg gtgtagccca
     9781 cggtgtccgg aatgttgatg gtggtggcac cggcgttaat cgccgcttca accacgcgcg
     9841 ccagatcggc aatgggtgtg cgtccggcat cttcgcaaga aaattcaaca tcatcggtgt
     9901 aattacgggc gcgtttcacc atatagatag cgcgttcgat tacttcgtcc agcgtgctac
     9961 gcagcttggt ggcaatgtgc attggtgaag tggcaataaa ggtatggata cggaaggctt
    10021 cggcaacttt cagagattca gctgccacgt cgatatcctt ttccacgcag cgagctaacg
    10081 cacatacgcg gctgtttttg acctggcggg cgatggtctg caccgattcg aaatcgcccg
    10141 gcgaagagac ggggaaaccg acttccatca cgtcaacacc catacgctca agggccagcg
    10201 caatttgcag tttttctttc gcactcaggc ttgcctgtaa cgcctgttca ccgtcgcgca
    10261 atgtggtatc gaaaataata acttgctggc tcatggtttg ggtccttgtc tctttttgag
    10321 cgcctcgctt cgggcataaa aaaacccgcg caatggcgcg ggttttttgt ttgactgcgt
    10381 gctggcttaa tgctgaatgc cgctcactcg tctaccgcgc aaagaagatg cgtttagtag
    10441 tagtagaccg ataaagcgaa cgatgtgagt cattaaatca gctccagatg aatacgatat
    10501 gcttttagag ttactggata caaaaacgga tgtcaaccct gacgcaacaa aaacgttccg
    10561 ccagagtgag ttctgcattc gtcaaattag ctaattgtgc tgcggtggtt aaaacaagcg
    10621 atattaattt ctgcttaacc accgacgctt ttcatcggtt gacatatttc agaataaatt
    10681 tttgcatcta atcaacgagg aaaaggggga taaaatgcac gcgttacaaa acctatcttg
    10741 atgatttgta ttgaattata tgttttgcta attttttgat attcatttgg tgaatattat
    10801 tgttcgatta atgttaagaa ttaatgcatt aaacatataa attaattatt aaaaaagcac
    10861 atttaatcca ttttgtggat gattgagtat tcgcggtagt tatgattaga ttgttttcgc
    10921 aacaaaaaca ttatggatta ttatgctgcg gtaaatgact ctttactcgg caatgggttc
    10981 tgtttttata agaacccgta tctttatgtt ttcctaattt ttctcatttt cctttttctt
    11041 attttatatg catgataaat catattctcg aggattattt ctctgcattc caataaggga
    11101 aagggagtta agtgtgacag tggagttaag tatgccagag gtacaaacag atcaaccaga
    11161 gacggcggag ttaagcaagc cacaactacg catggtcgat ctcaacttat taaccgtttt
    11221 cgatgccgtg atgcaggagc aaaacatcac ccgtgccgct catgttctgg ggatgtcgca
    11281 acctgcggtc agtaacgcgg ttgcacgcct gaaggtgatg tttaatgacg agctttttgt
    11341 tcgttatggc cgtggcattc aaccgactgc tcgcgcattt caactttttg gttcagttcg
    11401 tcaggcattg caattagtac aaaatgaatt gccaggttca ggttttgaac ccgcgagcag
    11461 tgaacgtgta tttcatcttt gtgtttgcag tccgttagac agcattctga cctcgcagat
    11521 ttataatcac attgagcaaa ctgcgccaaa tatacatgtt atgttcaagt cttcattaaa
    11581 tcagaacact gaacatcaac tgcgttatca ggaaacggag tttgtgatta gttatgaaga
    11641 cttccatcgt cctgaattta ccagtgtgcc attatttaaa gatgaaatgg tgctggtagc
    11701 cagcaaaaat catccaacaa ttaaaggccc gttactgaaa catgatgttt ataacgaaca
    11761 acatgcggca gtttcgctcg atcgtttcgc gtcatttagt caaccgtggt atgacacggt
    11821 agataaacaa gccagtatcg cgtatcaggg catggcaatg atgagtgtac tgagcgtggt
    11881 gtcgcaaacg catctggtcg ctattgcgcc gcgttggttg gctgaagagt tcgctgaatc
    11941 cttagagtta caggtattac cgctgccgtt aaaacaaaac agcagaacct gttatctctc
    12001 ctggcatgaa gctgccgggc gcgataaagg ccatcagtgg atggaagagc aattagtctc
    12061 aatttgcaaa cgctaactga atgcagaata ggtcagacat caatgtctgg tttattctgc
    12121 attttttatt gaatgtagaa ttttattctg aatgtgtgtt ctctctattt taggattaat
    12181 taaaaaaata gagaaattgc tgtaagttgt aggattcagc cgatttatta tcaatttaat
    12241 cctctgtaat ggaggatttt atcgtttctt ttcacctttc ctcctgttta ttcttattac
    12301 accgtgttta tgtctctgtc tgctaattgc ttaagcaaga tcggacggtt aatgtgtttt
    12361 acacattttt tccgtcaaac agtgaggcag gccatggaga tgttgtctgg agccgagatg
    12421 gtcgtccgat cgcttatcga tcagggcgtt aaacaagtat tcggttatcc cggaggcgcg
    12481 gtccttgata tttacgatgc attgcatacc gtgggtggta tcgatcatgt tttagttcgt
    12541 catgagcagg cggcggtgca tatggccgat ggtctggcgc gcgcgaccgg ggaagtcggc
    12601 gtcgtgctgg taacgtcggg tccaggggcg accaatgcga ttactggcat tgccaccgct
    12661 tatatggatt ccattccctt agttgtcctt tccgggcagg tagcgacctc gttgataggc
    12721 tacgatgcct ttcaggagtg cgacatggtg gggatttctc gcccggtggt taaacacagt
    12781 tttctggtta agcaaacgga agacattccg caggtgctga aaaaggcttt ctggctggcg
    12841 gcaagtggtc gccctgggcc cgtggtcgtt gatttaccca aagatattct taatccggcg
    12901 aacaaattac cctacgtctg gccggagtcg gtcagtatgc gatcttacaa tcccacgacc
    12961 tccgggcata aagggcaaat taagcgcgct ctgcaaacgc tggtggcggc aaaaaaaccg
    13021 gttgtctatg taggcggtgg ggcaatcacg gcgggctgtc atcagcagct aaaagaaacg
    13081 gtggaggcgt tgaatctgcc cgttgtttcc tcattaatgg ggctgggggc gtttccggca
    13141 acgcatcgtc aggcactggg catgctggga atgcacggca cctacgaagc caatatgacg
    13201 atgcataacg cggatgtgat ttttgccgtc ggggtgcgtt ttgatgaccg aacgacgaac
    13261 aatctggcga agtactgccc aaatgccacg gttctgcata tcgacattga tcctacttcc
    13321 atttctaaaa ccgtgactgc tgatatcccg attgtggggg atgctcgcca ggtccttgaa
    13381 caaatgtttg aactcctgtc gcaagaatcc gtccatcaac cgctggatga gatccgcgac
    13441 tggtggcaac agattgaaca gtggcgcgct cgtcagtgcc tgaaatatga ctctcacagt
    13501 gaaaagatta aaccgcaggc ggtgatcgag actctttggc ggttgacgaa gggagacgct
    13561 tacgtgacgt ccgatgtcgg gcagcaccag atgtttgctg cgctttatta tccattcgac
    13621 aaaccgcgtc gctggatcaa ctctggcggc ctcggcacga tggggtttgg tttgcctgcg
    13681 gcactgggcg tcaaaatggc gttgccagaa gaaaccgtgg tttgcgtcac tggcgacggc
    13741 agtattcaga tgaacatcca ggaactgtct accgccttgc aatacgagtt gccggtactg
    13801 gtggtgaatc tcaataaccg ctatctgggg atggtgaaac agtggcagga catgatctat
    13861 tctggccgtc attcacaatc ttatatgcaa tcgctacccg atttcgttcg tctggcggaa
    13921 gcctatggtc acgtcgggat ccagatttct catccgcaag aactggaaag caaacttagc
    13981 gaggcgctgg aacaggtgcg caataatcgc ctggtgtttg ttgatgtcac cgtcgatggc
    14041 agtgagcacg tctacccgat gcagattcgc gggggcggaa tggatgaaat gtggttaagc
    14101 aaaacggaga gaacctgatt atgcgccgga tattatcagt cttactcgaa aatgaatcag
    14161 gcgcgttatc ccgcgtgatt ggcctttttt cccagcgtgg ctacaacatt gaaagcctga
    14221 ccgttgcgcc aaccgacgat ccgacattat cgcgtatgac catccagacc gtgggcgatg
    14281 aaaaagtgct tgagcagatc gaaaagcaat tacacaagct ggtcgatgta ttacgcgtga
    14341 gcgagttggg gcagggggcg catgttgagc gggaaatcat gctggtgaaa attcaggcca
    14401 gcggctacgg gcgtgacgaa gtgaaacgta atacggaaat attccgtggg caaattatcg
    14461 atgtcacacc ctcgctttat accgttcaat tagcaggtac cagcgataag cttgatgcat
    14521 ttttagcatc gattcgcgat gtggcgaaaa ttgtggaagt tgctcgctct ggtgtggtcg
    14581 gactttcgcg cggcgaaaaa ataatgcgtt gagaatgatc tcaatgcgca atttgcagcc
    14641 caacatgtca cgttgggctt tttttgcgaa atcagtggga acctggaata aaagcagttg
    14701 ccgcagttaa ttttctgcgc ttagatgtta atgaatttaa cccataccag tacaatggct
    14761 atggttttta cattttacgc aaggggcaat tgtgaaactg gatgaaatcg ctcggctggc
    14821 gggagtgtcg cggaccactg caagctatgt tattaacggc aaagcgaagc aataccgtgt
    14881 gagcgacaaa accgttgaaa aagtcatggc tgtggtgcgt gagcacaatt accacccgaa
    14941 cgccgtggca gctgggcttc gtgctggacg cacacgttct attggtcttg tgatccccga
    15001 tctggagaac accagctata cccgcatcgc taactatctt gaacgccagg cgcggcaaag
    15061 gggttatcaa ctgctgattg cctgctcgga agatcagccc gacaacgaaa tgcgctgcat
    15121 tgagcacctt ttacagcgtc aggttgatgc cattattgtt tcgacgtcgt tgccacctga
    15181 gcatcctttt tatcaacgct gggctaacga cccgttcccg attgtcgcgc tggaccgcgc
    15241 cctcgatcgt gaacacttca ccagcgtggt tggtgctgat caggatgatg ccgaaatgct
    15301 ggcggaagag ttacgtaagt ttcccgccga gacggtgctt tatcttggcg cgctgccgga
    15361 gctttctgtc agcttcctgc gtgaacaagg cttccgtact gcctggaaag atgatccgcg
    15421 cgaagtgcat ttcctttatg ccaacagcta tgagcgggag gcagctgccc agttattcga
    15481 aaaatggctg gaaacgcatc cgatgccgca ggcgctgttc acaacgtcat ttgcgctgtt
    15541 gcaaggggtg atggatgtca cgctacgtcg tgacggcaaa ctgccttctg acctggcaat
    15601 tgccaccttt ggcgataacg aactgctcga cttcttacag tgcccggtgc tggcagtggc
    15661 tcaacgtcac cgcgatgtcg cagagcgtgt gctggagatt gtcctggcaa gcctggacga
    15721 accgcgtaag ccaaaacctg gtttaacgcg cattaaacgt aatctctatc gccgcggcgt
    15781 gctcagccgt agctaagccg cgaacaaaaa tacgcgccag gtgaatttct ctctggcgcg
    15841 ttgagtacgg gaatggacat caatatcctt aaagtaaata agactattcc tggctattat
    15901 tgataaatgc ttttaaacct gcccgttaat tacctcacca gctgaaattc acaataatta
    15961 agtgatatcg atagcgcgtt tttgcattat tttgttacat gcagagatga attgccgatt
    16021 taacaaacac ttttctttgc ttttgcgcaa acccgctggc atcaagcgtc acacagacgc
    16081 aacagggact gttaaccggg gaagatatgt cctaaaatgc cgctcgcgtc gcaaactgac
    16141 actttatatt tgctgtggaa aatagtgagt cattttaaaa cggtgatgac gatgagggat
    16201 tttttcttac agctattcat aacgttaatt tgcttcgcac gttggacgta aaataaacaa
    16261 cgctgatatt agccgtaaac atcgggtttt ttacctcggt atgccttgtg actggcttga
    16321 caagcttttc ctccgctccg taaactcctt tcagtgggaa attgtggggc aaagtgggaa
    16381 taaggggtga ggctggcatg ttccggggag caacgttagt caatctcgac agcaaagggc
    16441 gcttatcagt gcctacccgt tatcgggaac agctgcttga gaacgctgcc ggtcaaatgg
    16501 tttgcaccat tgacattcat cacccgtgcc tgctgcttta ccccctgcct gaatgggaaa
    16561 ttatcgagca aaaattatcg cgtctgtcga gcatgaaccc ggttgagcgc cgtgtgcagc
    16621 gcctactgtt aggtcatgcc agcgaatgtc agatggatgg cgcaggtcga ttgttaatcg
    16681 cgccagtact gcggcaacat gccgggctga caaaagaagt gatgctggtt ggacagttca
    16741 acaagtttga gctgtgggat gaaacaacct ggcatcaaca ggtcaaggaa gatatcgacg
    16801 cagagcagtt ggctaccgga gacttatcgg agcgactgca ggacttgtct ctataaaatg
    16861 atggaaaact ataaacatac tacggtgctg ctggatgaag ccgttaatgg cctcaatatc
    16921 cgtcctgatg gcatctacat tgatgggact tttggtcgcg gtggtcactc acgtctgatc
    16981 ctctcgcagc ttggcgaaga ggggcgtttg ttggcgatcg atcgcgaccc gcaggctatc
    17041 gccgttgcga agactattga tgatccgcgc ttctccatca tccacggacc tttctccgcg
    17101 ctgggcgaat acgttgccga gcgcgatctt atcggcaaga tcgatggcat tctcctcgat
    17161 cttggcgtct cttcaccgca acttgatgat gctgaacgcg gtttttcctt tatgcgcgat
    17221 ggtccgctgg acatgcgtat ggacccaacc cgtgggcagt cagccgctga atggctacaa
    17281 accgcagaag aagccgatat cgcctgggta ttgaaaacct atggtgaaga gcgttttgcc
    17341 aaacgcattg cccgcgccat tgtcgagcga aaccgcgaac agccgatgac ccgcaccaaa
    17401 gaactggcgg aagtcgtggc tgctgcaacg ccggtgaaag ataagtttaa acatcccgcg
    17461 acccggacct tccaggcggt gcgcatttgg gtaaacagtg aactggagga gatagagcag
    17521 gcgctaaaaa gctcgctcaa cgtgctggcc ccgggtgggc ggctttcgat catcagcttc
    17581 cactcgctgg aagaccgtat tgtgaaacgt tttatgcgtg aaaacagccg cggtccgcaa
    17641 gttccggcag ggttaccgat gactgaagag cagctcaaaa aactgggtgg ccgtcagctg
    17701 cgagcactag gcaagttaat gccgggcgaa gaagaggtgg ctgagaatcc tcgtgcccgt
    17761 agttcagttc tgcgtattgc agagaggacg aacgcatgat cagcagagtg acagaagctc
    17821 taagcaaagt taaaggatcg atgggaagcc acgagcgcca tgcattgcct ggtgttatcg
    17881 gtgacgatct tttgcgattt gggaagctgc cactctgcct gttcatttgc attattttga
    17941 cggcggtgac cgtggtaacc acggcgcacc atacccgttt actgaccgct cagcgcgaac
    18001 aactggtgct ggagcgagat gctttagaca ttgaatggcg caacctaatc cttgaagaga
    18061 atgcgctcgg cgaccatagc cgggtggaaa ggatagccac ggaaaagctg caaatgcagc
    18121 atgttgatcc gtcacaagaa aatatcgtag tgcaaaaata aggataaacg cgacgcatga
    18181 aagcagcggc gaaaacgcag aaaccaaaac gtcaggaaga acatgccaac tttatcagtt
    18241 ggcgttttgc gttgttatgc ggctgtattc tcctggcgct ggcttttctg ctcggacgcg
    18301 tagcgtggtt acaagttatc tccccggata tgctggtaaa agagggcgac atgcgttctc
    18361 ttcgcgttca gcaagtttcc acctcccgcg gcatgattac tgaccgttct ggtcgcccgt
    18421 tagcggtgag cgtgccggtt aaagcgattt gggctgaccc gaaagaagtg catgacgctg
    18481 gcggtatcag cgtcggtgat cgctggaagg cgctggctaa cgcactcaac attccgctgg
    18541 atcagctttc agcccgcatt aacgccaacc cgaaagggcg ctttatttat ctggcgcgtc
    18601 aggtaaaccc tgacatggca gagtacatca aaaaactgaa actgccgggg attcatctgc
    18661 gtgaagaatc tcgccgttat tatccatccg gcgaagtgac cgctcacctc atcggcttta
    18721 ctaacgtcga tagtcagggg attgagggcg tcgagaagag tttcgataaa tggcttaccg
    18781 ggcagccggg tgagcgcatt gtgcgtaaag accgctatgg ccgcgtaatt gaagatatct
    18841 cttccaccga cagtcaggcg gcgcacaacc tggcgctgag tattgatgaa cgcctgcagg
    18901 cgctggttta tcgcgaactg aacaacgcgg tggcctttaa caaagctgaa tctggtagcg
    18961 ccgtgctggt ggatgtcaac accggtgaag tgctggcgat ggctaacagc ccgtcataca
    19021 acccaaacaa tctgagtggc acgccgaaag aggcgatgcg taaccgtacc atcactgacg
    19081 tgtttgaacc gggttcaacg gttaaaccga tggtggtgat gaccgcgttg caacgtggcg
    19141 tagtgcggga aaactcggta ctgaataccg ttccttatcg aattaacggc cacgaaatca
    19201 aagacgtggc acgctacagc gaattaaccc tgaccggggt attacagaag tcgagtaacg
    19261 tcggtgtttc caagctggcg ttagcgatgc cgtcctcagc gttagtagat acttactcac
    19321 gttttggact gggaaaagcg accaatttgg ggttggtcgg agaacgcagt ggcttatatc
    19381 ctcaaaaaca acggtggtct gacatagaga gggccacctt ctctttcggc tacgggctaa
    19441 tggtaacacc gttacagtta gcgcgagtct acgcaactat cggcagctac ggcatttatc
    19501 gcccactgtc gattaccaaa gttgatcccc cggttcccgg tgaacgtgtc ttcccggaat
    19561 ccattgttcg caccgtggtg catatgatgg aaagcgtggc gctaccgggc ggtggcggcg
    19621 tgaaggcggc gattaaaggc tatcgtatcg ccattaaaac cggtaccgcg aaaaaggtcg
    19681 ggccggacgg tcgctacatc aataaatata ttgcttatac cgcaggcgtt gcgcctgcga
    19741 gtcagccgcg cttcgcgctg gttgttgtta tcaacgatcc gcaggcgggt aaatactacg
    19801 gcggcgccgt ttccgcgccg gtctttggtg ccatcatggg cggcgtattg cgtaccatga
    19861 acatcgagcc ggatgcgctg acaacgggcg ataaaaatga atttgtgatt aatcaaggcg
    19921 aggggacagg tggcagatcg taatttgcgc gaccttcttg ctccgtgggt gccagacgca
    19981 ccttcgcgag cactgcgaga gatgacactc gacagccgtg tggctgcggc gggcgatctc
    20041 tttgtagctg tagtaggtca tcaggcggac gggcgtcgat atatcccgca ggcgatagcg
    20101 caaggtgtgg ctgccattat tgcagaggcg aaagatgagg cgaccgacgg tgaaatccgt
    20161 gaaatgcacg gcgtaccggt catctatctc agccagctca acgagcgttt atctgcactg
    20221 gcgggccgct tttaccatga accctctgat aatttacgtc ttgtaggcgt aacgggcacc
    20281 aacggcaaaa ccacgacgac ccagctgctg gcacagtgga gccaactgct tggcgaaacc
    20341 agcgcggtaa tgggcaccgt tggtaacggc ctgctgggga aagtgatccc gacagaaaat
    20401 accaccggtt cggcagtcga tgttcagcat gagctggcgg ggctggtgga tcagggcgcg
    20461 actttttgcg caatggaagt ctcttcccac gggctggtac agcatcgcgt ggcggcgctg
    20521 aaatttgccg catcggtctt taccaactta agccgcgatc accttgatta tcatggtgat
    20581 atggaacact acgaagccgc gaaatggctg ctttattctg aacatcgttg cggtcaggcg
    20641 attattaacg ccgacgatga agtgggccgc cgctggctgg caaaactgcc ggacgcggtt
    20701 gcggtatcaa tggaagatca tatcaatctg aactgtcacg gacgctggtt gaaagcgacc
    20761 gaagtgaact atcacgacag cggtgcgacg attcgcttta gctcaagctg gggcgatggc
    20821 gaaattgaaa gccatctgat gggcgctttt aacgtcagca acctgctgct tgcgctggcg
    20881 acactgctgg cactcggcta cccgctcgct gatctgttaa aaaccgccgc gcgtctgcaa
    20941 ccggtttgcg gacgtatgga agtgttcact gcgccaggca aaccgacggt ggtggtggat
    21001 tacgcgcata cgccggatgc actggaaaaa gccttacagg cggcgcgcct gcactgtgcg
    21061 ggcaagctgt ggtgcgtctt tggctgtggt ggcgatcgtg ataaaggcaa acgtccactg
    21121 atgggcgcaa ttgctgaaga gtttgctgac gtggcggtgg taacggacga taacccgcgt
    21181 accgaagaac cgcgtgccat catcaacgat attctggcgg gaatgttaga tgccggacat
    21241 gccaaagtga tggaaggccg tgctgaagcg gtgacttgcg ccgttatgca ggctaaagag
    21301 aatgatgtgg tactggtcgc gggcaaaggt catgaggatt accagattgt tggcaatcag
    21361 cgtctggact actccgatcg cgtcacggtg gcgcgtctgc tgggggtgat tgcatgatta
    21421 gcgtaaccct tagccaactt accgatattc tcaacggtga actgcaaggt gcagatatta
    21481 cccttgatgc tgtaaccact gacacgcgaa aactgacgcc gggctgcctg tttgttgccc
    21541 tgaaaggcga acgtttcgat gctcatgatt ttgccgacca ggcgaaagct ggcggcgcag
    21601 gcgcactact ggttagccgt ccgctggata tcgatctgcc gcagttaatc gtcaaggata
    21661 cgcgtctggc gtttggtgaa ctggctgcat gggttcgcca gcaagttccg gcgcgcgtgg
    21721 ttgctctgac aggttcctcc ggcaaaacat ccgttaaaga gatgacggcg gcgattttaa
    21781 gccagtgcgg caacacgctt tatacggcag gcaatctcaa caacgacatc ggcgtaccga
    21841 tgacgctgtt gcgcttaacg ccggaatacg attacgcagt tattgaactt ggcgcgaacc
    21901 atcagggcga aattgcctgg actgtgagtc tgactcgccc ggaagcggcg ctggtcaaca
    21961 acctggcagc ggcacatctg gaaggttttg gctcgcttgc gggtgtcgcg aaagcgaaag
    22021 gtgaaatctt tagcggcctg ccggaaaacg gtatcgccat catgaacgct gacaacaacg
    22081 actggctgaa ctggcagagc gtaattggct cacgcaaagt gtggcgtttc tcacccaatg
    22141 ccgccaacag cgatttcacc gccaccaata tccatgtgac ttcgcacggt acggaattta
    22201 ccctgcaaac cccaaccggt agcgtggatg ttctgctgcc gttgccgggg cgtcacaata
    22261 ttgcgaatgc gctggcagcc gctgcgctct ccatggccgt gggcgcaacg cttgatgcta
    22321 tcaaagcggg gctggcaaat ctgaaagctg ttccaggccg tctgttcccc attcaactgg
    22381 tagaaaacca gttgctgctc gacgactcct acaacgccaa tgttggttca atgactgcag
    22441 cagtccaggt actggctgaa atgccgggct accgcgtgct ggtggtgggc gatatggcgg
    22501 aactgggcgc tgaaagcgaa gcctgccata tacaggtggg cgaagcggca aaagcagctg
    22561 gtattgaccg cgtgttaagc gtgggcaaac aaagccatgc tatcagcacc gccagcggcg
    22621 ttggcgaaca tttttccgat aaaactgcgc ttatcgcgcg tcttaaatca ctgattgctg
    22681 agcaacaggt aattacgatt ttagttaagg gttcacgtag tgctgccatg gaagaggtag
    22741 tacgcgcttt acaggagaat gggacatgtt agtttggctg gccgaacatt tggtcaaata
    22801 ttattccggc tttaacgtct tttcctatct gacgtttcgc gccatcgtca gcctgctgac
    22861 tgcgctgttc atctcattgt ggatgggccc gcgcatgatt gctcatttgc aaaaactttc
    22921 ctttggtcag gtggtgcgta acgacggtcc tgaatcacac ttcagcaagc gcggtacgcc
    22981 gaccatgggc gggattatga tcctgacagc gattgtgatc tccgtactgc tgtgggctta
    23041 tccatccaat ccgtacgtct ggtgcgtact ggtggtgctg gtaggctacg gcatcattgg
    23101 ctttgttgat gactatcgca aagtggtgcg taaagacacc aaagggttga tcgctcgttg
    23161 gaagtatttc tggatgtcgg tcattgcgct gggtgttgcc ttcgcgctgt accttgccgg
    23221 caaagacacg cccgcaacgc agctggtggt tccgttcttt aaagatgtga tgccgcaact
    23281 ggggctgttc tacattctgc tggcttactt cgtcattgtg ggtacaggca acgcggtaaa
    23341 cctgactgat ggtcttgacg gtctggcaat tatgccgacc gtatttgtcg ccggtggttt
    23401 tgcgctggtg gcgtgggcga ccggcaacat gaactttgcc agctatttac acataccgta
    23461 tctacgacac gccggggaac tggtcattgt ctgtaccgcg atagtcgggg cagggctggg
    23521 cttcctgtgg tttaacacct atccggcgca ggtctttatg ggcgatgtcg gttcgctggc
    23581 gttaggtggt gcgttaggta ttatcgccgt actgctgcgt caggaattcc tgctggtgat
    23641 tatggggggc gtgttcgtgg tagaaacact ttccgtcatc ctgcaggtcg gctcctttaa
    23701 actgcgcgga caacgtattt tccgcatggc accgattcat caccactatg aactgaaagg
    23761 ctggccggaa ccgcgcgtca ttgtacgttt ctggattatt tcgctgatgc tggtcctgat
    23821 tggtctggca acgctgaagg tacgttaatc atggctgatt accagggtaa aaatgtcgtc
    23881 attatcggcc tgggcctcac cgggctttct tgcgtggact ttttcctcgc tcgcggtgtg
    23941 acgccgcgcg ttatggatac gcgtatgaca ccgcctggcc tggataaatt acccgaagct
    24001 gtagaacgcc acacgggcgg tctgaatgat gaatggctga tggcggccga tctgattgtc
    24061 gccagtcccg gtattgcact ggcgcatcca tccttaagcg ctgccgctga tgccggaatc
    24121 gaaatcgttg gcgatatcga gctgttctgt cgtgaagcac aagcaccgat tgtggcgatc
    24181 accggttcta acggcaaaag cacggtcacc acgctagtgg gtgaaatggc gaaagcggcg
    24241 ggggttaacg ttggtgtggg tggcaatatt ggcctgcctg cgttgatgct gctggatgat
    24301 gagtgcgaac tgtacgtgct ggagctgtcg agcttccagc ttgaaaccac ctccagttta
    24361 caggcggtag cagcgaccat tctgaacgta actgaagatc atatggatcg ctatccgttt
    24421 ggtttacaac agtatcgtgc agcaaaactg cgcatttacg aaaacgcaaa aatttgcgtg
    24481 gttaatgctg atgatgcctt aacaatgccg attcgcggtg cggatgaacg ctgcgtcagc
    24541 tttggcgtca acatgggtga ctatcacctg aatcatcagc agggtgaaac ctggctgcgg
    24601 gtgaagggcg agaaagtgct gaacgtgaaa gaaatgaaac tttccgggca gcataactac
    24661 accaatgcac tggcagctct ggcactggca gatgccgctg gtttgccgcg cgccagcagc
    24721 ctgaaagcgt taaccacatt cactggtctg ccgcatcgct ttgaagttgt gctggagcat
    24781 aacggtgtgc gctggattaa cgattcaaaa gcgaccaacg tcggcagtac ggaagcggca
    24841 ctgaatggcc tgcacgtaga cggcacgctg catttgttgc tgggcggcga tggtaaatcg
    24901 gcagatttta gcccactggc gcgttacctg aatggcgata acgtacgtct gtattgtttc
    24961 ggtcgggacg gcgcgcagct ggcggcgcta cgcccggaag tggcagaaca aaccgaaacc
    25021 atggaacagg cgatgcgctt gctggctccg cgtgttcagc cgggcgatat ggttctgctc
    25081 tccccggcct gtgccagcct tgatcagttc aagaactttg aacaacgagg caatgagttt
    25141 gcccgtctgg cgaaggagtt aggttgatgc gtttatctct ccctcgcctg aaaatgccgc
    25201 gcctgccagg attcagtatt ctggtctgga tctccacggc gctaaagggc tgggtgatgg
    25261 gctcgcggga aaaagatacc gacagcctga tcatgtacga tcgcacctta ctgtggctga
    25321 ccttcggcct cgcggcgatt ggctttatca tggtgacctc ggcgtcaatg cccatagggc
    25381 aacgcttaac caacgatccg ttcttcttcg cgaagcgtga tggcgtctat ctgatcctgg
    25441 cattcattct ggcgatcatt acgctgcgtc tgccgatgga gttctggcaa cgctacagtg
    25501 ccacgatgct gctcggatct atcatcctgc tgatgatcgt tctggtagtg ggtagctcgg
    25561 ttaaaggggc atcgcgttgg atcgatctcg gcttgctgcg tatccagcct gcggagctga
    25621 caaaactgtc gttgttttgc tatatcgcca actatctggt gcgtaaaggc gacgaagtac
    25681 gtaataacct gcgcggcttt ctgaaaccga tgggcgtaat tctggtgttg gcagtgctac
    25741 tgctggcaca gccagacctt ggtacggtgg tggtgttgtt tgtgactacg ctggcgatgt
    25801 tgttcctggc gggagcgaaa ttgtggcagt tcattgccat tatcggtatg ggcatttcag
    25861 ctgttgtgtt gctgatcctc gccgaaccgt accgtatccg ccgtgttacc gcattctgga
    25921 atccgtggga agatcccttt ggcagcggct atcagttaac gcaatcgttg atggcgtttg
    25981 gtcgcggtga actgtgggga caaggtttag gtaactcggt acaaaaactg gagtatctgc
    26041 cggaagcgca cactgacttt attttcgcca ttatcggcga agaactgggg tatgtcggtg
    26101 tggtgctggc acttttaatg gtattcttcg tcgcttttcg cgcgatgtcg attggccgca
    26161 aagcattaga aattgaccac cgtttttccg gttttctcgc ctgttctatt ggtatctggt
    26221 ttagtttcca ggcgctggtt aacgtaggcg cggcggcggg gatgttaccg accaaaggtc
    26281 tgacattgcc gctgatcagt tacggtggtt cgagcttact gattatgtcg acagccatca
    26341 tgatgctgtt gcgtattgat tatgaaacgc gtctggagaa agcgcaggcg tttgtacgag
    26401 gttcacgatg agtggtcaag gaaagcgatt aatggtgatg gcaggcggaa ccggtggaca
    26461 tgtattcccg ggactggcgg ttgcgcacca tctaatggct cagggttggc aagttcgctg
    26521 gctggggact gccgaccgta tggaagcgga cttagtgcca aaacatggca tcgaaattga
    26581 tttcattcgt atctctggtc tgcgtggaaa aggtataaaa gcactgatag ctgcgccgct
    26641 gcgtatcttc aacgcctggc gtcaggcgcg ggcgattatg aaagcgtaca aacctgacgt
    26701 ggtgctcggt atgggcggct atgtatcagg tccaggtggt ctggctgcgt ggtcgttagg
    26761 cattccggtt gtacttcatg aacaaaacgg tattgcgggc ttaaccaata aatggctggc
    26821 gaagattgct accaaagtga tgcaggcgtt tccaggcgct ttccctaatg cggaagtggt
    26881 gggtaacccg gtgcgtaccg atgtgctggc gctgccgttg ccgcagcaac gtttggctgg
    26941 acgtgaaggt ccggttcgtg tgttggtagt gggtggttcc cagggcgcac gcattcttaa
    27001 tcagacaatg ccgcaggttg ctgcaaaact gggtgattca gtcactatct ggcatcagag
    27061 cggcaaaggt tcgcaacaat ccgttgaaca ggcgtatgcc gaagcgggac aaccgcagca
    27121 taaagtgacg gaatttattg atgatatggc ggcggcgtat gcgtgggcgg atgtcgttgt
    27181 ttgccgctcc ggtgcgttaa cggtgagtga aatcgccgcg gcaggacttc cggcgttgtt
    27241 tgtgccgttt caacataaag accgtcagca atactggaat gcgctaccgc tggaaaaagc
    27301 gggcgcagcc aaaattatcg agcagccaca gcttagcgtg gatgctgtcg ccaacaccct
    27361 ggccgggtgg tcgcgagaaa ccttattaac catggcagaa cgcgcccggg ctgcatccat
    27421 tccggatgcc accgagcgag tggcaaatga agtgagccgg gctgcccggg cgtaattgta
    27481 gcgatgcctt ttgcatcgta tgaatttaag aagttaatgg cgtaaagaat gaatacacaa
    27541 caattggcaa aactgcgttc catcgtgccc gaaatgcgtc gcgttcggca catacatttt
    27601 gtcggcatcg gtggtgccgg tatgggcggt attgccgaag ttctggccaa tgaaggttat
    27661 cagatcagtg gttccgattt agcgccaaat ccggtcacgc agcagttaat gaatctggga
    27721 gcgacgattt atttcaacca tcgcccggaa aacgtacgtg atgccagcgt ggtcgttgtt
    27781 tccagcgcga tttctgccga taacccggaa attgttgcag ctcatgaagc gcgtattccg
    27841 gtgatccgtc gtgctgaaat gctggctgag ttaatgcgtt ttcgtcatgg catcgccatt
    27901 gccggaacac acggcaaaac gacaaccacc gcgatggttt ccagcatcta cgcagaagcg
    27961 gggctcgacc caaccttcgt taacggcggg ctggtaaaag cggcgggggt tcatgcgcgt
    28021 ttggggcatg gtcggtacct gattgccgaa gcagatgaga gtgatgcatc gttcctgcat
    28081 ctgcaaccga tggtggcgat tgtcaccaat atcgaagccg accacatgga tacctaccag
    28141 ggcgactttg agaatttaaa acagactttt attaattttc tgcacaacct gccgttttac
    28201 ggtcgtgcgg tgatgtgtgt tgatgatccg gtgatccgcg aattgttacc gcgtgtggga
    28261 cgtcagacca cgacttacgg cttcagcgaa gatgccgacg tgcgtgtaga agattatcag
    28321 cagattggcc cgcaggggca ctttacgctg ctgcgccagg acaaagagcc gatgcgcgtc
    28381 accctgaatg cgccaggtcg tcataacgcg ctgaacgccg cagctgcggt tgcggttgct
    28441 acggaagagg gaattgacga cgaggctatt ttgcgtgcgc tggagagctt ccaggggacg
    28501 gggcgccgtt tcgatttcct cggtgaattc ccgctggagc cagtgaatgg aaaaagcggt
    28561 acggcaatgc tggtcgatga ctacggccac cacccgacgg aagtggacgc cactattaaa
    28621 gcggcgcgcg caggctggcc ggataaaaac ctggtaatgc tgtttcagcc gcaccgtttt
    28681 acccgtacgc gcgacctgta tgatgatttc gccaatgtgc tgacgcaggt tgataccctg
    28741 ttgatgctgg aagtgtatcc ggctggtgaa gcgccaattc cgggagcgga cagccgttcg
    28801 ctgtgtcgca caattcgtgg acgtgggaaa attgatccca ttctggtgcc ggacccggcg
    28861 caggtagccg agatgctggc accggtatta accggtaacg acctgattct cgttcagggg
    28921 gctggtaata tcggaaaaat tgctcgttct ttagctgaaa tcaaactgaa gccgcaaact
    28981 ccggaggaag aacaacatga ctgataaaat cgcggtcctg ttgggcggga cctccgctga
    29041 gcgggaagtt tctctgaatt ctggcgcagc ggtgttagcc gggctgcgtg aaggcggtat
    29101 tgacgcgtat cctgtcgacc cgaaagaagt cgacgtgacg caactgaagt cgatgggttt
    29161 tcagaaagtg tttatcgcgc tacacggtcg cggcggtgaa gatggtacgc tgcagggaat
    29221 gctcgagctg atgggcttgc cttataccgg cagcggagtg atggcatctg cgctttcaat
    29281 ggataaacta cgcagcaaac ttctgtggca gggtgccggt ttaccggtcg cgccgtgggt
    29341 agcgttaacc cgcgcagagt ttgaaaaagg ccttaacgat cagcagttag cagaaatttc
    29401 ttctctgggt ttgccggtta tcgttaagcc gagccgcgaa ggttccagtg tgggaatgtc
    29461 aaaagtagta gcagaaaatg ctctacaaga tgcattaaga ttggcatttc agcacgatga
    29521 agaagtattg attgaaaaat ggctaagtgg gccggagttc acggttgcga tactcggtga
    29581 agaaatttta ccgtcaatac gtattcaacc ggccggaacc ttctatgatt atgaggcgaa
    29641 gtatctctct gatgagacac agtatttctg ccccgcaggt ctggaagcgt tacaagaggc
    29701 caatttgcag gcattagtgc taaaagcatg gacgacgtta ggttgcaaag gatggggacg
    29761 tattgacgtt atgctggaca gtgatggaca gttttatctg ctggaagcca atacctcacc
    29821 gggtatgacc agccacagtc tggtgccgat ggcggcacgt caggcaggta tgagcttctc
    29881 gcagttggta gtacgaattc tggaactggc ggactaatat gtcgcaggct gctctgaaca
    29941 cgcgaaatag cgaagaagag gtttcttctc gccgcaataa tggaacgcgt ctggcgggga
    30001 tccttttcct gctgaccgtt ttaacgacag tgttggtgag cggctgggtc gtgttgggct
    30061 ggatggaaga tgcgcaacgc ctgccgctct caaagctggt gttgaccggt gaacgccatt
    30121 acacgcgtaa tgacgatatc cggcagtcga tcctggcatt gggtgagcca ggtaccttta
    30181 tgacccagga tgtcaacatc atccagacgc aaatagaaca acgcctgccg tggattaagc
    30241 aggtgagcgt cagaaagcag tggcctgatg aattgaagat tcatctggtt gaatatgtgc
    30301 cgattgcgcg gtggaatgat caacatatgg tagacgcgga aggaaacact ttcagcgtgc
    30361 cgccagatcg caccagcaag caggtgcttc caatgctgta tggcccggaa ggcagcgcca
    30421 atgaagtgtt gcagggctat cgcgaaatgg ggcagatgct ggcaaaggac agatttactc
    30481 tgaaggaagc ggcgatgacc gcgcggcgtt cctggcagtt gacgctgaat aacgatatta
    30541 agctcaatct tggccggggc gatacgatga aacgtttggc tcgctttgta gaactttatc
    30601 cggttttaca gcagcaggcg caaaccgatg gcaaacggat tagctacgtt gatttgcgtt
    30661 atgactctgg agcggcagta ggctgggcgc ccttgccgcc agaggaatct actcagcaac
    30721 aaaatcaggc acaggcagaa caacaatgat caaggcgacg gacagaaaac tggtagtagg
    30781 actggagatt ggtaccgcga aggttgccgc tttagtaggg gaagttctgc ccgacggtat
    30841 ggtcaatatc attggcgtgg gcagctgccc gtcgcgtggt atggataaag gcggggtgaa
    30901 cgacctcgaa tccgtggtca agtgcgtaca acgcgccatt gaccaggcag aattgatggc
    30961 agattgtcag atctcttcgg tatatctggc gctttctggt aagcacatca gctgccagaa
    31021 tgaaattggt atggtgccta tttctgaaga agaagtgacg caagaagatg tggaaaacgt
    31081 cgtccatacc gcgaaatcgg tgcgtgtgcg cgatgagcat cgtgtgctgc atgtgatccc
    31141 gcaagagtat gcgattgact atcaggaagg gatcaagaat ccggtaggac tttcgggcgt
    31201 gcggatgcag gcaaaagtgc acctgatcac atgtcacaac gatatggcga aaaacatcgt
    31261 caaagcggtt gaacgttgtg ggctgaaagt tgaccaactg atatttgccg gactggcatc
    31321 aagttattcg gtattgacgg aagatgaacg tgaactgggt gtctgcgttg tcgatatcgg
    31381 tggtggtaca atggatatcg ccgtttatac cggtggggca ttgcgccaca cgaaggtaat
    31441 tccttatgct ggcaatgtcg tgaccagtga tatcgcttac gcctttggca caccgccaag
    31501 tgacgccgaa gcgattaaag ttcgccacgg ttgtgcgctg ggctccatcg ttggaaaaga
    31561 tgagagcgtg gaagtgccga gcgtaggcgg ccgtccgccg cggagtctgc aacgtcagac
    31621 cctggcagag gtgatcgagc cgcgctatac cgagctgctc aatctggtca acgaagagat
    31681 attgcagttg caggaaaagc ttcgccaaca aggggttaaa catcacctgg cggcaggcat
    31741 tgtattaacc ggtggcgcag cgcagatcga aggtcttgca gcctgtgctc agcgcgtgtt
    31801 tcatacgcaa gtgcgtatcg gcgcgccgct gaacattacc ggtttaacgg attatgctca
    31861 ggagccgtat tattcgacgg cggtgggatt gcttcactat gggaaagagt cacatcttaa
    31921 cggtgaagct gaagtagaaa aacgtgttac agcatcagtt ggctcgtgga tcaagcgact
    31981 caatagttgg ctgcgaaaag agttttaatt ttttatgagg ccgacgatga ttacggcctc
    32041 aggcgacagg cacaaatcgg agagaaacta tgtttgaacc aatggaactt accaatgacg
    32101 cggtgattaa agtcatcggc gtcggcggcg gcggcggtaa tgctgttgaa cacatggtgc
    32161 gcgagcgcat tgaaggtgtt gaattcttcg cggtaaatac cgatgcacaa gcgctgcgta
    32221 aaacagcggt tggacagacg attcaaatcg gtagcggtat caccaaagga ctgggagctg
    32281 gcgctaatcc agaagttggc cgcaatgcgg ctgatgagga tcgcgatgca ctgcgtgcgg
    32341 cgctggaagg tgcagacatg gtctttattg ctgcgggtat gggtggtggt accggtacag
    32401 gtgcagcacc agtcgtcgct gaagtggcaa aagatttggg tatcctgacc gttgctgtcg
    32461 tcactaagcc cttcaacttt gaaggcaaga agcgtatggc attcgcggag caggggatca
    32521 ctgaactgtc caagcatgtg gactctttga tcactatccc gaacgacaaa ctgctgaaag
    32581 ttctgggccg cggtatctcc ctgctggatg cgtttggcgc agcgaacgat gtactgaaag
    32641 gtgctgtaca aggtatcgcc gaactgatta cgcgtccggg tttgatgaac gtggactttg
    32701 cagacgttcg caccgtaatg tctgaaatgg gctacgcaat gatgggttct ggcgtggcga
    32761 gcggtgaaga ccgtgcggaa gaagctgctg aaatggctat ctcttctccg ctgctggaag
    32821 atatcgacct gtctggcgcg cgcggcgtgc tggttaacat cacagcgggc ttcgacctgc
    32881 gtctggatga gttcgaaacg gtaggtaaca ccatccgtgc atttgcttcc gacaacgcga
    32941 ctgtggttat cggtacttct cttgacccgg atatgaatga cgagctgcgc gtaactgttg
    33001 ttgcgacagg tatcggcatg gacaaacgtc ctgaaatcac tctggtgacc aataagcagg
    33061 ttcagcagcc agtgatggat cgctaccagc agcacgggat ggctccgctg acccaggagc
    33121 agaagccggt tgctaaagtc gtgaatgaca atgcgccgca aactgcgaaa gagccagatt
    33181 atctggatat cccagcattc ctgcgtaagc aagctgatta agaattgact ggaatttggg
    33241 tttcgaggct ctttgtgcta aactggcccg ccgaatgtat agtacacttc ggttggatag
    33301 gtaatttggc gagataatac gatgatcaaa caaaggacac ttaaacgtat cgttcaggcg
    33361 acgggtgtcg gtttacatac cggcaagaaa gtcaccctga cgttacgccc tgcgccggcc
    33421 aacaccgggg tcatctatcg tcgcaccgac ttgaatccac cggtagattt cccggccgat
    33481 gccaaatctg tgcgtgatac catgctctgt acgtgtctgg tcaacgagca tgatgtacgg
    33541 atttcaaccg tagagcacct caatgctgct ctcgcgggct tgggcatcga taacattgtt
    33601 atcgaagtta acgcgccgga aatcccgatc atggacggca gcgccgctcc gtttgtatac
    33661 ctgctgcttg acgccggtat cgacgagctg aactgcgcca agaaatttgt tcgcatcaaa
    33721 gagactgttc gtgtcgaaga tggcgataag tgggctgaat ttaagccgta caatggtttt
    33781 tcgctggatt tcaccatcga ttttaaccat ccggctattg attccagcaa ccagcgctat
    33841 gcgatgaact tctccgctga tgcgtttatg cgccagatca gccgtgcgcg tacgttcggt
    33901 ttcatgcgtg atatcgaata tctgcagtcc cgtggtttgt gcctgggcgg cagcttcgat
    33961 tgtgccatcg ttgttgacga tt
//
LOCUS       DAMZFT010000041        33738 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.041, whole genome shotgun sequence.
ACCESSION   DAMZFT010000041 DAMZFT010000000
VERSION     DAMZFT010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 33738)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33738)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33738
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.041"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(<1..1058)
                     /gene="codB"
                     /locus_tag="PN977_002059"
     CDS             complement(<1..1058)
                     /gene="codB"
                     /locus_tag="PN977_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine permease"
                     /protein_id="HDI6042088.1"
                     /translation="MSQDNNFSQGPVPQSARKGVLALTFVMLGLTFFSASMWTGGTLG
                     TGLSYHDFFLAVLIGNLLLGIYTSFLGYIGAKTGLTTHLLARFSFGVKGSWLPSLLLG
                     GTQVGWFGVGVAMFAIPVGKATGLDINLLIAVSGLLMTVTVFFGISALTVLSVIAVPA
                     IACLGGYSVWLAVNGMGGLDALKAVVPAQPLDFNVALALVVGSFISAGTLTADFVRFG
                     RNAKLAVLVAMVAFFLGNSLMFIFGAAGAAALGMADISDVMIAQGLLLPAIVVLGLNI
                     WTTNDNALYASGLGFANITGMSSKTLSVINGIIGTVCALWLYNNFVGWLTFLSAAIPP
                     VGGVIIADYLMNRRRYEH"
     gene            complement(1295..3181)
                     /gene="prpE"
                     /locus_tag="PN977_002060"
     CDS             complement(1295..3181)
                     /gene="prpE"
                     /locus_tag="PN977_002060"
                     /EC_number="6.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionate--CoA ligase"
                     /protein_id="HDI6042089.1"
                     /translation="MSFSEFYQRSINEPEKFWAEQARRIDWQTPFTQTLDHSNPPFAR
                     WFCEGRTNLCHNAIDRWLEKQPEALALIAVSSETEEERTFTFRQLHDEVNAVASMLRS
                     LGVRRGDRVLVYMPMIAEAHITLLACARIGAIHSVVFGGFASHSVAARIDDAKPVLIV
                     SADAGARGGKIIPYKKLLDDAISQAHHQPRHVLLVDRGLAKMARVTGRDVDFASLRHQ
                     HIGARVPVAWLESNETSCILYTSGTTGKPKGVQRDVGGYAVALATSMDTIFGGKAGGV
                     FFCASDIGWVVGHSYIVYAPLLAGMATIVYEGLPTWPDCGVWWKIVEKYQVSRMFSAP
                     TAIRVLKKFPTAEIRKHDLSSLEVLYLAGEPLDEPTASWVSNTLDVPVIDNYWQTESG
                     WPIMAIARGLDDRPTRLGSPGVPMYGYNVQLLNEVTGEPCGVNEKGMLVVEGPLPPGC
                     IQTIWGDDDRFVKTYWSLFSRPVYATFDWGIRDADGYHFILGRTDDVINVAGHRLGTR
                     EIEESISSHPGVAEVAVVGVKDALKGQVAVAFVIPKESDSLEDREVAHSQEKAIMALV
                     DSQIGNFGRPAHVWFVSQLPKTRSGKMLRRTIQAICEGRDPGDLTTIDDPASLDQIRQ
                     AMEE"
     gene            complement(3221..4672)
                     /gene="prpD"
                     /locus_tag="PN977_002061"
     CDS             complement(3221..4672)
                     /gene="prpD"
                     /locus_tag="PN977_002061"
                     /EC_number="4.2.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-methylcitrate dehydratase"
                     /protein_id="HDI6042090.1"
                     /translation="MSAQINNIRPEFDREIVDIVDYVMNYEISSRVAYDTAHYCLLDT
                     LGCGLEALEYPACKKLLGPIVPGTVVPNGVRVPGTQFQLDPVQAAFNIGAMIRWLDFN
                     DTWLAAEWGHPSDNLGGILATADWLSRNAVASGKAPLTMKQVLTAMIKAHEIQGCIAL
                     ENSFNRVGLDHVLLVKVASTAVVAEMLGLTRDEILNAVSLAWVDGQSLRTYRHAPNTG
                     TRKSWAAGDATSRAVRLALMAKTGEMGYPSALTAPVWGFYDVSFKGESFRFQRPYGSY
                     VMENVLFKISFPAEFHSQTAVEAAMTLYEQMQAAGKTAADIEKVTIRTHEACIRIIDK
                     KGPLNNPADRDHCIQYMVAIPLLFGRLTAADYEDNVAQDKRIDALREKINCFEDPAFT
                     ADYHDPEKRAIANAITLEFTDGTRFEEEVVEYPIGHARRRQDGIPKLVDKFKINLARQ
                     FPTRQQQRILEVSLDRARLEQMPVNEYLDLYVI"
     gene            complement(4706..5875)
                     /gene="prpC"
                     /locus_tag="PN977_002062"
     CDS             complement(4706..5875)
                     /gene="prpC"
                     /locus_tag="PN977_002062"
                     /EC_number="2.3.3.16"
                     /EC_number="2.3.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-methylcitrate synthase"
                     /protein_id="HDI6042091.1"
                     /translation="MSDTTILQNSTHVIKPKKSVALSGVPAGNTALCTVGKSGNDLHY
                     RGYDILDLAEHCEFEEVAHLLIHGKLPTRDELAAYKTKLKALRGLPANVRTVLEALPA
                     ASHPMDVMRTGVSALGCTLPEKEGHTVSGARDIADKLLASLSSILLYWYHYSHNGERI
                     QPETDDDSIGGHFLHLLHGEKPSQSWEKAMHISLVLYAEHEFNASTFTSRVIAGTGSD
                     MYSAIIGAIGALRGPKHGGANEVSLEIQQRYETPDEAEADIRKRVENKEVVIGFGHPV
                     YTIADPRHQVIKRVAKQLSQEGGSLKMYNIADRLETVMWESKKMFPNLDWFSAVSYKM
                     MGVPTEMFTPLFVIARVTGWAAHIIEQRQDNKIIRPSANYVGPEDRQFVALDKRQ"
     gene            complement(5920..6810)
                     /gene="prpB"
                     /locus_tag="PN977_002063"
     CDS             complement(5920..6810)
                     /gene="prpB"
                     /locus_tag="PN977_002063"
                     /EC_number="4.1.3.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylisocitrate lyase"
                     /protein_id="HDI6042092.1"
                     /translation="MSLHSPGKAFRAALSKETPLQIVGTINANHALLAQRAGYQAIYL
                     SGGGVAAGSLGLPDLGISTLDDVLTDIRRITDVCSLPLLVDADIGFGSSAFNVARTVK
                     SMIKAGAAGLHIEDQVGAKRCGHRPNKAIVSKEEMVDRIRAAVDAKTDPDFVIMARTD
                     ALAVEGLDAAIERAQAYVEAGAEMLFPEAITELAMYRQFADAVQVPILANITEFGATP
                     LFTTDELRSAHVAMALYPLSAFRAMNRAAEHVYNVLRQEGTQKSVIDTMQTRNELYES
                     INYYQYEEKLDDLFARNQAK"
     gene            7049..8635
                     /gene="prpR"
                     /locus_tag="PN977_002064"
     CDS             7049..8635
                     /gene="prpR"
                     /locus_tag="PN977_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionate catabolism operon regulatory protein
                     PrpR"
                     /protein_id="HDI6042093.1"
                     /translation="MAHPPRLNDDKPVIWTVSVTRLFELFRDISLEFDHLANITPIQL
                     GFEKAVTYIRKKLANERCDAIIAAGSNGAYLKSRLSVPVILIKPSGYDVLQALAKAGK
                     LTSSIGVVTYQETIPALVAFQKTFNLRLDQRSYITEEDARGQINELKANGTEAVVGAG
                     LITDLAEEAGMTGIFIYSAATVRQAFSDALDMTRMSLRHNTHDATRNALRTRYVLGDM
                     LGQSPQMEQVRQTILLYARSSAAVLIEGETGTGKELAAQAIHREYFARHDARQGKKSH
                     PFVAVNCGAIAESLLEAELFGYEEGAFTGSRRGGRAGLFEIAHGGTLFLDEIGEMPLP
                     LQTRLLRVLEEKEVTRVGGHQPVPVDVRVISATHCNLEEDMRQGEFRRDLFYRLSILR
                     LQLPPLRERVTDILPLAESFLKVSLAALSAPFSAALRQGLQASETVLVHYDWPGNIRE
                     LRNMMERLALFLSVEPTPDLTPQFLQLLLPELARESAKTPAPRLLTPQQALEKFNGDK
                     TAAANYLGISRTTFWRRLKS"
     gene            complement(8736..9011)
                     /locus_tag="PN977_002065"
     CDS             complement(8736..9011)
                     /locus_tag="PN977_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HDI6042094.1"
                     /translation="MKIISKMLVGALAFAVTNVYAAELMTKAEFEKVASQYEKIGDIS
                     TSNEMSTADAKEDLIKKADEKGADVLVLTSGQTDNKIHGTANIYKKK"
     gene            9158..9829
                     /locus_tag="PN977_002066"
     CDS             9158..9829
                     /locus_tag="PN977_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HDI6042095.1"
                     /translation="MMKLLHLFMDEITMDPLHAVYLTVGLFVITFFNPGANLFVVVQT
                     SLASGRRAGVLTGLGVALGDAFYSGLGLFGLATLITQCEEIFSLIRIVGGAYLLWFAW
                     CSMRRQSTPQMSTLQQPINAPWYVFFRRGLITDLSNPQTVLFFISIFSVTLNAETPTW
                     ARLMAWAGIVLASIIWRVFLSQAFSLPAVRRAYGRMQRVASRIIGAIIGVFALRLIYE
                     GVTQR"
     gene            complement(9846..10091)
                     /locus_tag="PN977_002067"
     CDS             complement(9846..10091)
                     /locus_tag="PN977_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414861.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6042096.1"
                     /translation="MAVQLFITLLNQIPLLSSLQSETLPLFGYSGRVRAMKKAHTGES
                     GLKWEAKDSSKLIGNKDHALRGLSSPMVGIRQIRLIT"
     gene            complement(10505..11320)
                     /locus_tag="PN977_002068"
     CDS             complement(10505..11320)
                     /locus_tag="PN977_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6042097.1"
                     /translation="MISLKAPHNNLMPYTQQSILNTVKNNQLSEDIKSSLVSCVDIFK
                     VLIKQYYDYPYDCRDDLVDDDKLIHLMAAVRDCEWSDDNALTINVQFNDFPGFYDWMD
                     YPDHPVKFVFRILENQKGTVWVYDQDDAFLDIKANVQAGRFTGLKKLEQFIDSVRTDC
                     KCILLEHHMPLLRLFPKGKECMHVEKWLREMSSIPETDAPIKQALAHGLLLHLKNIYP
                     VFPESLVRLLLSVLDVKTYRDDARLNEWISNRVQELGDRYYPVNKHVKIRYTL"
     gene            complement(11563..12612)
                     /gene="yahK"
                     /locus_tag="PN977_002069"
     CDS             complement(11563..12612)
                     /gene="yahK"
                     /locus_tag="PN977_002069"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent aldehyde reductase YahK"
                     /protein_id="HDI6042098.1"
                     /translation="MKIKAVGAYSAKQPLEPMDITRREPGPHDVKIEIAYCGVCHSDI
                     HQVRSEWAGTVYPCVPGHEIVGRVVAVGDQVEKHAPGDLVGVGCIVDSCKHCEECEDG
                     LENYCDHMTGTYNSPTPDEPGHTLGGYSQQIVVHERYVLRIRHPQEQLAAVAPLLCAG
                     ITTYSPLRHWQAGPGKKVGVVGIGGLGHMGIKLAHAMGAHVVAFTTSEAKREAAKALG
                     ADEVVNSRNADEMAAHLKSFDFILNTVAAPHNLDDFTTLLKRDGTMTLVGAPATPHKS
                     PEVFNLIMKRRAIAGSMIGGIPETQEMLDFCAEHDIVADIEMIRADQINEAYERMLRG
                     DVKYRFVIDNRTLTD"
     gene            complement(12989..14371)
                     /locus_tag="PN977_002070"
     CDS             complement(12989..14371)
                     /locus_tag="PN977_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HDI6042099.1"
                     /translation="MKENNSRREFLSQSGKMVTAAALFGTSVPLAHAGVAGTLNCEAN
                     NTMKITDPHYYLDNVLLETGFDYENGVAVQTRTARQTVEIQDGKIVALRENKQHPDAT
                     LPHYDAGGKLMLPTTRDMHIHLDKTFYGGPWRSLNRPAGTTIQDMIKLEQKMLPELQP
                     YTQERVEKLIDLLQSKGTTIARSHCNIEPVSGLKNLQNLQAVLARRQAGFECEIVAFP
                     QHGLLLSKSEALMREAMQAGAHYVGGLDPTSVDGAMEKSLDTMFQIALDYDKGVDIHL
                     HETTPSGVAAINYMVETVEKTPQLKGKLTISHAFALATLNEQQVDELAHRMAAQQISI
                     ASTVPIDTLHMPLKQLHDKGVKVMTGTDSVIDHWSPYGLGDMLEKANLYAQLYIRPNE
                     QNLSRSLFLATGDVLPLNEKGERVWPKAQDDASFVLVDASCSAEAVARISPRTATFHK
                     GQLVWGSVAG"
     gene            complement(14381..15331)
                     /locus_tag="PN977_002071"
     CDS             complement(14381..15331)
                     /locus_tag="PN977_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase family protein"
                     /protein_id="HDI6042100.1"
                     /translation="MKELVVVAIGGNSIIKDNASQSIEHQAEAVKAVADTVLEMLASD
                     YDIVLTHGNGPQVGLDLRRAEIAHEREGLPLTPLANCVADTQGGIGYLIQQALNNRLA
                     RHGEKKAVTVVTQVEVDKNDPGFAHPTKPIGAFFSESQRDELQKANPDWRFVEDAGRG
                     YRRVVASPEPKRIVEAPAIKALIQQGFVVIGAGGGGIPVVRTEAGDYQSVDAVIDKDL
                     STALLAREIHADILVITTGVEKVCIHFGKPQQQVLDRVDIATMTRYMQEGHFPPGSML
                     PKIIASLTFLEQGGKEVIITTPECLPAALRGETGTHIIKT"
     gene            complement(15426..16844)
                     /locus_tag="PN977_002072"
     CDS             complement(15426..16844)
                     /locus_tag="PN977_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HDI6042101.1"
                     /translation="MSQSLFSQPLNVINVGIAMFSDDLKKQHVEVTQLDWTPPGQGNM
                     QVVQALDNIADSPLADKIAAANQQAPERIIQSHPVLIGFDQAINVVPGMTPKTILHAG
                     PPVTWEKMCGAMKGAVTGALVFEGLAKDLDEAAELAASGEITFSPCHEHDCVGSMAGV
                     TSASMFMHIVKNKTYGNIAYTNMSEQMAKILRMGANDQSVIDRLNWMRDVQGPMLRDA
                     MKIIGEIDLRLMLAQALHMGDECHNRNNAGTTLLIQALTPGIIQAGYSVEQQREVFEF
                     VASSDYFSGPTWMAMCKAAMDAAHGIEYSTVVTTMARNGVEFGLRVSGLPGQWFTGPA
                     QQVIGPMFAGYKPEDSGLDIGDSAITETYGIGGFAMATAPAIVALVGGTVEEAIDFSR
                     QMREITLGENPNVTIPLLGFMGVPSAIDITRVGSSGILPVINTAIAHKDAGVGMIGAG
                     IVHPPFACFEKAILGWCERYGV"
     gene            complement(16844..18391)
                     /gene="fdrA"
                     /locus_tag="PN977_002073"
     CDS             complement(16844..18391)
                     /gene="fdrA"
                     /locus_tag="PN977_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase FdrA"
                     /protein_id="HDI6042102.1"
                     /translation="MSVKIVIKPNTYFDSVSLMSISTRANKLDGVEQAFVAMATEMNK
                     GVLKNLGLLTPELEQAKNGDLMIVINGKAGADNEQLLVEIEELFNTKAQSGSHEARYA
                     TIASAKKHIPESNLAVISVNGLFAAREARQALQNDLNVMLFSDNVSVEDELALKQLAH
                     EKGLLLMGPDCGTAIINGAALCFGNAVRRGNIGIVGASGTGSQELSVRIHEFGGGVSQ
                     LIGTGGRDLSEKIGGLMMFDAIGMLENDPQTEIIVLISKPPAPAVARKVLERARACRK
                     PVVVCFLGRGETPVDEQGLQFARGSKEAALKAVMLSGVKQEHLDLHTLNQPLIADVRA
                     RLKPKQKYIRGLFCGGTLCDETMFAVMEKHGDVYSNIQPDPEFRLKDINRSVKHTFLD
                     FGDDDFTNGKPHPMIDPTNRISRLIEEARDPEVALIVMDFVLGFGSHEDPVGSTIEAI
                     KEAKAIAAAEGRELIILAYVLGTDLDTPSLEQQSQMLLDAGVILASSSTNTGLLAREF
                     ICKGEEA"
     gene            complement(18381..19244)
                     /locus_tag="PN977_002074"
     CDS             complement(18381..19244)
                     /locus_tag="PN977_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
                     /protein_id="HDI6042103.1"
                     /translation="MWALTADADFLAQRGQGQVEQLFARAVNIALPARQQLLTLLCEE
                     YDNAPNSCRLALTHFDGLFRHGDKVQFDDQGITVGQHLHIEMSRCRLWLSPTLQMTAV
                     NFHFIAWQQWHDIIHQHLGENETLFNYRGDSPFYQALNKELHIKRRAVIQAVNDKQNI
                     ASAVASMMGLGIGLTPSADDYLTGLALVLFISGHPAEKYKEEFYLGLQRGRNNTTLLS
                     AITLEAALQQRCRENIHRFIHNIIYDIPGNATQAIEKIKHIGSSSGCDMLYGMADGCA
                     LSQTYGGNYVS"
     gene            complement(19284..19889)
                     /locus_tag="PN977_002075"
     CDS             complement(19284..19889)
                     /locus_tag="PN977_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HDI6042104.1"
                     /translation="MTIKNLPADYLLAAQQGDIDKVKTCLALGVDINTCDRQGKTAIT
                     LASLYQQYACVQALIDAGADINKQDQICSNPFLISCLTGDLTLLRIILPAKPDLNCVT
                     RFGGVGLTPACEKGHLSIVKELLAHTEINVNQTNHVGWTPLLEAIVLNDGGIKQQAIV
                     QLLLEHGASPHMTDKYGKTPLELARERGFEEIAQLLIAAGA"
     gene            20147..20644
                     /locus_tag="PN977_002076"
     CDS             20147..20644
                     /locus_tag="PN977_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HDI6042105.1"
                     /translation="MNGLTATGVTVGICAGLWQLVSSHVGLSQGWELLGTIGFVAFCS
                     FYAAGGGKSGFIRSLAVNYSGMVWAFFAALAAGWLASVSGLSTFWASVIMTVPFSAVV
                     VWQGRFGLLSFIPGGFLGMTLFFASGMNWTVTLLGFLAGNCVGIISEYGGQKLSEATT
                     KRDGY"
     gene            20736..21668
                     /locus_tag="PN977_002077"
     CDS             20736..21668
                     /locus_tag="PN977_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDI6042106.1"
                     /translation="MNSIFTEENLLAFTTAARFGSFSKAAEELGLTTSAISYTIKRME
                     TGLDVVLFTRSTRSIELTESGRYFFRKATDLLNDFHAIKRSIDTISQGIEARVRICIN
                     QLLYTPKHTARLLQVLKKQFPTCQITVTTEVYNGVWDAIINNQANIAIGAPDTLLDGG
                     GIDYTEIGAIRWAFAIAPDHPLAFVPEPIAESQLRLYPNIMVEDTAHTINKKVGWLLH
                     GQESILVPDFNTKCQCQILGEGIGFLPDYMVREAMAQSLLVTRQIHNPRQDSRMLLAT
                     QHSATGQVTQWIKKQFAPNGILTGIYQDLLHREN"
     gene            complement(21710..22798)
                     /gene="pdeL"
                     /locus_tag="PN977_002078"
     CDS             complement(21710..22798)
                     /gene="pdeL"
                     /locus_tag="PN977_002078"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator/c-di-GMP
                     phosphodiesterase PdeL"
                     /protein_id="HDI6042107.1"
                     /translation="MNSCDFRVFLQEFGTTVHLSLPGSVSEKERLLLKLLMQGMSVTE
                     ISQYRNRSAKTISHQKKQLFEKLGIQSDITFWRDIFFQYNPEIISATGNNSHKYINDN
                     HYHHIVTPEAISLALENHEFKPWIQPVFCAQTGVLTGCEVLVRWEHPQTGIIPPDQFI
                     PLAESSGLIVIMTRQLMKQTADILMPVKHLLPDNFHIGINVSAGCFLAAGFEKECLNL
                     VKKLGNDKIKLVLELTERNPIPVTPEARAIFDSLHQHNITFALDDFGTGYATYRYLQA
                     FPVDFIKIDKSFVQMASVDEISGHIVDNIVELARKPGLSIVAEGVETQEQADLMIGKG
                     VHFLQGYLYSPPVPGNKFISEWVMKAGG"
     gene            complement(23674..25707)
                     /gene="betT"
                     /locus_tag="PN977_002079"
     CDS             complement(23674..25707)
                     /gene="betT"
                     /locus_tag="PN977_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline BCCT transporter BetT"
                     /protein_id="HDI6042108.1"
                     /translation="MTDLSHSREKDKINPVVFYTSAGLILLFSLTTILFRDFSALWIG
                     RTLDWVSKTFGWYNLLAATLYIVFVVCIACSRFGSVKLGPEQSKPEFSLLSWAAMLFA
                     AGIGIDLMFFSVAEPVTQYMQPPEGAGQTIEAARQAMVWTLFHYGLTGWSMYALMGMA
                     LGYFSYRYNLPLTIRSALYPIFGKRINGPIGHSVDIAAVIGTIFGIATTLGIGVVQLN
                     YGLSVLFDIPDSMAAKAALIALSVIIATISVTSGVDKGIRVLSELNVALALGLILFVL
                     FMGDTSFLLNALVLNVGDYMNRFMGMTLNSFAFDRPVEWMNNWTLFFWAWWVAWSPFV
                     GLFLARISRGRTIRQFVLGTLIIPFTFTLLWLSVFGNSALYEIIHGGAAFAEEAMVHP
                     ERGFYSLLAQYPAFTFSASVATITGLLFYVTSADSGALVLGNFTSQLKDINSDAPGWL
                     RVFWSVAIGLLTLGMLMTNGISALQNTTVIMGLPFSFVIFFVMAGLYKSLKVEDYRRE
                     SANRDTAPRPLGLQDRLSWKKRLSRLMNYPGTRYTKQMMETVCYPAMEEVAQELRLRG
                     AYVELKSLPPEEGQQLGHLDLLVHMGEEQNFVYQIWPQQYSVPGFTYRARSGKSTYYR
                     LETFLLEGSQGNDLMDYSKEQVITDILDQYERHLNFIHLHREAPGHSVMFPDA"
     gene            25836..26423
                     /gene="betI"
                     /locus_tag="PN977_002080"
     CDS             25836..26423
                     /gene="betI"
                     /locus_tag="PN977_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308386.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator BetI"
                     /protein_id="HDI6042109.1"
                     /translation="MPKLGMQSIRRRQLIDATLEAINEVGMHDATIAQIARRAGVSTG
                     IISHYFRDKNGLLEATMRDITSQLRDAVLNRLHALPQGSAEQRLQAIVGGNFDETQVS
                     SAAMKAWLAFWASSMHQPMLYRLQQVSSRRLLSNLVSEFHRELPRQQAQEAGYGLAAL
                     IDGLWLRAALSGKPLDKPLAHSLTRHFITQHLPTD"
     gene            26437..27909
                     /gene="betB"
                     /locus_tag="PN977_002081"
     CDS             26437..27909
                     /gene="betB"
                     /locus_tag="PN977_002081"
                     /EC_number="1.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="betaine-aldehyde dehydrogenase"
                     /protein_id="HDI6042110.1"
                     /translation="MSRMAEQQLYIHGGYTSATSGRTFETINPANGNVLATVQAAGRE
                     DVDRAVKSAQQGQKIWAAMTAMERSRILRRAVDILRERNDELAKLETLDTGKAYSETS
                     TVDIVTGADVLEYYAGLIPALEGSQIPLRETSFVYTRREPLGVVAGIGAWNYPIQIAL
                     WKSAPALAAGNAMIFKPSEVTPLTALKLAEIYSEAGLPDGVFNVLPGVGAETGQYLTE
                     HPGIAKVSFTGGVASGKKVMANSAASSLKEVTMELGGKSPLIVFDDADLDLAADIAMM
                     ANFFSSGQVCTNGTRVFVPAKCKAAFEQKILARVERIRAGDVFDPQTNFGPLVSFPHR
                     DNVLRYIAKGKEEGARVLCGGDVLKGDGFDNGAWVAPTVFTDCRDEMTIVREEIFGPV
                     MSLLTYESEDEVIRRANDTDYGLAAGIVTADLNLAHRVIHQLEAGICWINTWGESPAE
                     MPVGGYKHSGIGRENGVMTLQSYTQVKSIQVEMAKFQSIF"
     gene            27923..29611
                     /gene="betA"
                     /locus_tag="PN977_002082"
     CDS             27923..29611
                     /gene="betA"
                     /locus_tag="PN977_002082"
                     /EC_number="1.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline dehydrogenase"
                     /protein_id="HDI6042111.1"
                     /translation="MQFDYIIIGAGSAGNVLATRLTEDQNTTVLLLEAGGPDYRFDFR
                     TQMPAALAFPLQGKRYNWAYETEPEPFMNNRRMECGRGKGLGGSSLINGMCYIRGNAL
                     DLDNWAQEPGLENWSYLDCLPYYRKAETRDVGENDYHGGDGPVSVTTSKPGVNPLFEA
                     MIEAGVQAGYPRTDDLNGYQQEGFGPMDRTVTPHGRRASTARGYLDQAKSRSNLTIRT
                     HAMTDHIIFDGKRAVGVEWLEGDSTIPTRAAANKEVLLCAGAIASPQILQRSGVGNAG
                     LLAEFDIPLVHELPGVGENLQDHLEMYLQYECKEPVSLYPALQWWNQPKIGAEWLFGG
                     TGIGASNHFEAGGFIRSREEFAWPNIQYHFLPVAINYNGSNAVKEHGFQCHVGSMRSP
                     SRGHVRIKSRAPHQHPAILFNYMSHEQDWQEFRDAIRITREIMHQPALDQYRGREISP
                     GVECQTDEQLDEFVRNHAETAFHPCGTCKMGYDEMSVVDGEGRVHGLEGLRVVDASIM
                     PQIITGNLNATTIMIGEKMADMIRGKDALPRSTAGYYVANGMPVRAKKMSRDVN"
     gene            29872..30069
                     /locus_tag="PN977_002083"
     CDS             29872..30069
                     /locus_tag="PN977_002083"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6042112.1"
                     /translation="MNTNKTLGSNKQHSDRECLYKAEVTMTGKGTRFQEWMPVFFNLN
                     ILALVMPGYLQKSSITMTERG"
     gene            complement(30143..30838)
                     /locus_tag="PN977_002084"
     CDS             complement(30143..30838)
                     /locus_tag="PN977_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414842.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein C"
                     /protein_id="HDI6042113.1"
                     /translation="MDNRSEFLNNVAQALGRPQRLEPQAEDAPLNNYANERLTELNQQ
                     QRCDAFIQFASDVMLTRCELTSEAQAAEAAIRLCKELGDQSIVISGDTRLEELGISER
                     LQQECNAVVWDPAKGIENISQAEQAKVGVVYAEYGLTESGGVVLFSAAGRGRSLSLLP
                     ESSLFILRKSTILPRVAQLAEKLHQKAQAGERMPSCINIISGPSSTADIELIKVVGVH
                     GPVKAVYLIIEDC"
     gene            complement(30831..32258)
                     /locus_tag="PN977_002085"
     CDS             complement(30831..32258)
                     /locus_tag="PN977_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster-binding protein"
                     /protein_id="HDI6042114.1"
                     /translation="MSIKTSNTDFKTRIRQQIEDPIMRKAVANAQQRIGANRQKMVDE
                     LGHWEEWRDRAAQIRDHVLSNLDAYLYQLSEKVTQNGGHVYFAKTKEDATRYILQVAQ
                     RKNARKVVKSKSMVTEEIGVNHVLQDAGIQVIETDLGEYILQLDQDPPSHVVVPAIHK
                     DRHQIRRVLHERLGYEGSETPEAMTLFIRQKIREDFLSAEIGITGCNFAVAETGSVCL
                     VTNEGNARMCTTLPKTHIAVMGMERIAPTFAEVDVLITMLARSAVGARLTGYNTWLTG
                     PREAGHVDGPEEFHLVIVDNGRSEVLASEFRDVLRCIRCGACMNTCPAYRHIGGHGYG
                     SIYPGPIGAVISPLLGGYKDFKDLPYACSLCTACDSVCPVRIPLSKLILRHRRVMAEK
                     GITAKAEQRAIKMFAYANSHPGLWKVGMMAGAHAASWFINGGKTPLKFGAISDWMEAR
                     DLPEADGESFRSWFKKHQAQEKKNG"
     gene            complement(32269..32988)
                     /locus_tag="PN977_002086"
     CDS             complement(32269..32988)
                     /locus_tag="PN977_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HDI6042115.1"
                     /translation="MNVNFFVTCIGDALKSRMARDSVLLLEKLGCRVNFPEKQGCCGQ
                     PAINSGYIKEAIPGMKNLIAALEDNDDPIISPAGSCTYAVKSYPTYLADEPEWASRAE
                     KVAARMQDLTSFIVNTLGVVDVGASLQGRAVYHPSCSLARKLGVKDEPLTLLKNVRGL
                     ELLTFAEQDTCCGFGGTFSVKMAEISGEMVQEKVAHLMEVRPEYLIGADVSCLLNISG
                     RLQREGQKVKVMHIAEVLMSR"
     gene            complement(33514..>33738)
                     /gene="rclR"
                     /locus_tag="PN977_002087"
     CDS             complement(33514..>33738)
                     /gene="rclR"
                     /locus_tag="PN977_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RclR"
                     /protein_id="HDI6042116.1"
                     /translation="AQLFRDVSGTTPLAVLTKLRLQIAAQMFSREMLPVVVIAESVGY
                     ASESSFHKAFVREFGCTPGEYRERVRQLAP"
BASE COUNT         8130 a         9063 c         8405 g         8140 t
ORIGIN      
        1 aagtgctcat agcggcgacg gttcatcaga tagtcagcga tgatcacgcc ccctaccgga
       61 ggaatagctg ccgaaaggaa ggtcagccag ccgacaaaat tgttatacag ccataatgcg
      121 cagaccgtac cgataatacc gttgattacc gaaagggttt tgctcgacat cccggtaatg
      181 ttggcgaaac ctaagcccga cgcatagagc gcgttatcgt tggtggtcca gatattcagc
      241 cccagcacca caatcgcagg cagcagcaga ccctgagcaa tcatcacatc agagatatcc
      301 gccatcccca gtgccgccgc gcccgctgca ccgaaaataa acatcaacga gttgccgagg
      361 aaaaaggcca ccatcgccac cagcaccgcc agtttggcat tacgaccaaa ccggacaaag
      421 tcagcggtga gcgtgcccgc actgataaat gaccccacaa ccagcgccag cgcgacattg
      481 aaatctaacg gttgtgcggg aacgaccgct tttaatgcgt ccaggccgcc catgccgtta
      541 acggccaacc acacggaata accgccaaga caggcgatag ccggaactgc aatcaccgaa
      601 agaaccgtca gcgccgaaat gccaaagaag acggttacgg tcatcagtaa accggaaacg
      661 gcaatcagca aattaatatc cagcccagtt gccttgccca ccggaatagc aaacatcgcc
      721 acgccaacgc caaaccagcc aacctgagtt ccgcccagta gcagtgaagg cagccatgag
      781 cctttaacac caaaagagaa gcgagcaaga agatgagtgg tcaggccggt ttttgcgcca
      841 atgtaaccaa gaaatgaagt gtaaataccg aggagaagat taccgatgag gactgcgagg
      901 aagaaatcat gatagctaag accggttccg agagtgccgc cggtccacat actggcggaa
      961 aagaaggtta atcccagcat gacgaacgtc aatgccaata cccctttccg cgccgactgc
     1021 gggactggcc cctggctaaa gttgttatct tgcgacacga aattccccct ttttgctgtt
     1081 tgaaacccaa aaaatccgcc gcattctatt catctgaaga taaaaagcaa tcgttttcgt
     1141 aggggaatat attttttata tgaatgagtg caaaaaggtg ggggaaaaat tatcggtgta
     1201 aaaacagtgg gtaggcataa taagacgcgg taagcgtcgc atcaggcatc ggcgcatggt
     1261 gtcggatgcg ccaggcgacg catccgacaa taacctactc ttccatcgcc tggcggatct
     1321 gatccaacga cgcaggatca tcaatggtcg tcagatcccc aggatcgcgt ccttcgcaaa
     1381 tcgcctggat cgtgcggcgc agcatttttc cggatcgcgt tttcggcaat tgcgagacaa
     1441 accagacgtg cgccgggcgg ccaaagttgc caatctggct gtccaccagc gccataatcg
     1501 ccttctcttg cgaatgcgcc acctcacgat cttccagact gtcgctctct ttcggaatga
     1561 caaacgccac cgccacctgc cctttcagcg catctttcac cccaaccacc gccacttcgg
     1621 caacgcccgg atgactggag atactctctt caatctcgcg cgtccccagc cgatgcccgg
     1681 caacgttaat cacatcatca gtacgcccca ggataaagtg atagccgtca gcatcgcgga
     1741 tgccccagtc aaaagtggcg tacaccggac gggaaaacag cgaccagtac gtcttcacaa
     1801 agcggtcgtc gtcgccccag atggtctgaa tacagcctgg cggcaatggc ccctccacca
     1861 ccagcatccc tttctcattg acgccacacg gttcgccggt gacttcatta agcaactgca
     1921 cgttatagcc atacatcggc acgccggggc ttcccagacg cgtcggcctg tcgtccagac
     1981 cgcgagcaat cgccataatc ggccagccgg attcggtctg ccagtagttg tcgatgaccg
     2041 gcacatccag cgtattgctc acccaactgg cggtcggctc gtccagcggt tctccagcca
     2101 gatagagcac ttccagcgac gagagatcgt gtttgcgaat ttcagcggta gggaattttt
     2161 tcagcacgcg aatggcggtc ggcgctgaga acatccgact aacctgatat ttctcgacga
     2221 ttttccacca cacgccgcag tccggccagg tcggtaatcc ttcgtaaacg atagtcgcca
     2281 tccccgccag cagcggcgcg tagacgatat acgaatgccc caccacccag ccgatatccg
     2341 acgcacagaa gaacacaccg cccgctttgc cgccaaaaat ggtgtccatc gaggtcgcca
     2401 gcgccaccgc atatccgcca acgtcacgtt gcacgccttt aggtttgccg gtcgtgccgg
     2461 aggtgtagag aatgcaggag gtttcgttgg attccagcca cgccaccggt acccgcgcgc
     2521 cgatgtgttg atggcgcaat gacgcgaaat cgacatcccg tccggtaacg cgcgccattt
     2581 tcgccagccc gcgatccacc agtaaaacgt ggcgcggctg atgatgcgcc tggcttatcg
     2641 catcgtcgag caatttttta tacggaataa ttttgccacc gcgcgctccg gcatcagccg
     2701 agacaatcag caccggttta gcatcatcaa ttcgcgccgc cacgctgtgc gaggcaaatc
     2761 caccaaacac caccgagtga atagcgccaa tgcgcgcgca ggccagcaga gtaatatgcg
     2821 cttcggcaat catcggcata tacaccagca cccgatcgcc acgccgtacg cccagtgagc
     2881 gcagcattga cgccactgcg ttcacttcgt catgcagctg acgaaaggta aaggtgcgct
     2941 cttcttctgt ttccgaagag acggcaatca gcgccagcgc ctctggctgt ttctccagcc
     3001 agcggtcgat agcgttgtga cacaagttgg ttcggccttc acaaaaccaa cgggcaaacg
     3061 gcggattgct gtgatcaagc gtttgcgtaa agggcgtctg ccagtcaata cgccgggcct
     3121 gctcggccca gaacttctcc ggttcgttaa tcgaacgctg ataaaattcg ctaaaagaca
     3181 taatgctctc ctgttgaact tacgccttac cgctgtttgc ttaaatgacg tacaggtcga
     3241 gatactcatt gaccggcatc tgttccaggc gagctctgtc gagagaaacc tccagaatgc
     3301 gctgctgttg gcgagtcggg aactggcgcg cgagattgat tttgaattta tcgaccagct
     3361 tcggaattcc atcctgacgg cggcgagcat gaccaattgg gtactccacc acctcttctt
     3421 caaagcgcgt gccgtcggtg aactcaaggg ttatggcatt ggcgatggcg cgtttttccg
     3481 ggtcgtggta gtcagcggta aacgccggat cttcaaagca attgatcttc tcgcgcaggg
     3541 cgtcgatgcg tttatcttgc gcaacgttgt cctcgtaatc tgccgccgtt aagcgcccga
     3601 acagcagcgg gatcgccacc atgtactgaa tgcagtggtc gcggtcagcc gggttattga
     3661 gcggcccttt tttgtcgatg atgcgaatac aggcttcgtg ggtgcgaatg gtcacttttt
     3721 cgatatccgc cgccgttttg cctgctgcct gcatctgttc atagagcgtc atcgccgctt
     3781 caactgccgt ctgggagtgg aactccgccg ggaaggagat tttgaacagc acattctcca
     3841 tgacgtaaga accgtacggg cgctggaagc ggaacgattc acctttaaag gagacgtcgt
     3901 agaaacccca cacaggcgca gtcagggctg acgggtagcc catttcgccc gttttcgcca
     3961 tcagtgccag acgtaccgcg cgggacgtgg catcgcccgc cgcccaggat ttacgcgtgc
     4021 cggtgttcgg cgcatggcga taggtgcgca gcgactgacc gtccacccac gccagcgaaa
     4081 cagcgttgag gatttcatca cgggtcaggc caagcatttc ggcgaccacg gcggtggaag
     4141 ccactttcac taacagaacg tggtcaaggc cgacgcgatt aaaggagttt tccagcgcga
     4201 tgcagccctg aatttcatgg gctttaatca ttgcggtcag cacctgtttc atggtcaacg
     4261 gcgctttgcc gctggcgacc gcgttgcgcg aaagccagtc cgccgttgcc agaatgccgc
     4321 cgaggttgtc ggaaggatgg ccccactccg ccgccagcca ggtatcgttg aaatcgagcc
     4381 aacggatcat cgcgccgatg ttaaatgccg cctggacggg gtcgagctgg aactgagttc
     4441 cggggacgcg cacgccgtta ggtacgacgg tgccggggac aattggcccc agcagttttt
     4501 tacaggctgg gtattcgaga gcttcaagac cgcagccgag cgtgtcgagc aggcagtaat
     4561 gtgcagtgtc gtaggctact ctggagctga tttcgtagtt catcacgtaa tcgacgatat
     4621 caacgatttc acgatcaaat tccgggcgga tgttgttgat ttgagctgac attgggtacg
     4681 tttccttatt gttattcgta gaggtttact ggcgcttatc cagcgcgaca aactggcggt
     4741 cttccggtcc aacataattg gcggaaggac ggataatttt gttgtcctga cgttgttcga
     4801 taatgtgcgc cgcccagccg gtgacgcggg cgataacaaa cagtggtgtg aacatctcgg
     4861 tgggaacgcc catcattttg taggaaacag cggagaacca gtcgagattg gggaacatct
     4921 ttttgctctc ccacatcacc gtttccaggc gatcggcgat gttgtacatc ttcagcgagc
     4981 cgccttcctg cgagagctgc ttcgccacgc gtttgatcac ctgatgacgc gggtcagcga
     5041 tggtataaac cggatgtcca aaaccaatca ccacttcttt gttttccacg cgcttgcgga
     5101 tatcggcttc ggcttcgtcc ggcgtttcgt aacgttgctg gatctccagc gacacttcat
     5161 tcgccccgcc gtgtttcggc ccgcgcagtg cgccaatcgc gccgataatc gctgaataca
     5221 tgtcagagcc ggtgcccgca atcacccggc tggtaaaggt ggaggcgtta aactcgtgtt
     5281 cggcgtacag caccagcgaa atatgcatcg ccttttccca gctttgcgac ggcttttcac
     5341 catgcagcag atgcaggaaa tgaccgccta tagaatcgtc atcggtttcc ggctggatgc
     5401 gttcgccgtt gtggctgtag tgataccagt agagaagaat cgagctcagc gacgccaaca
     5461 gtttgtcggc aatatcacgc gcgccggaga cggtatgccc ctctttttct ggcagcgtgc
     5521 agccgagcgc ggaaacgccg gtgcgcataa catccatcgg gtgtgacgcc gccggtaagg
     5581 cttccagcac ggtacgcacg ttagccggta aaccgcgcag ggctttcagt ttcgttttgt
     5641 aggcggcgag ttcgtcacgg gtcggcagtt tgccgtggat cagcagatgc gctacttctt
     5701 caaattcgca atgttccgcc agatcaagaa tgtcgtagcc gcggtaatgc aaatcattgc
     5761 cgcttttacc cacggtgcag agcgctgtat tgcccgccgg aacgccagaa agcgccaccg
     5821 attttttcgg tttaatgaca tgggtactgt tttgcaggat cgttgtgtcg ctcatgttgt
     5881 cctcgtcatt tgtagggtcg agatattttt cgttgtgttt tatttcgcct ggttacgggc
     5941 aaacaggtcg tcgagcttct cttcgtactg gtagtagttg atgctttcgt acagctcgtt
     6001 gcgggtctgc atggtgtcga tgacgctttt ctgcgtgcct tcctgacgca ggacgttgta
     6061 gacatgttcg gcggcgcggt tcatggcacg gaacgctgaa agtgggtaca gcgccattgc
     6121 gacatgggcg ctgcgtaatt cgtcggtggt aaacagcggt gttgcgccaa attcggtaat
     6181 gttggcgagg attggcacct gcaccgcatc agcaaactgg cggtacatgg cgagttcggt
     6241 aatcgcctcc gggaacaaca tctcggcacc cgcttcaaca taggcctgcg cgcgctcgat
     6301 cgcggcgtcc agcccctcta ccgccagcgc atcggtgcgc gccatgatca caaaatcagg
     6361 atcggttttc gcatccaccg ccgcgcggat ccgatccacc atctcctctt tcgagacgat
     6421 cgctttgttc ggacgatgac cgcagcgttt cgcaccaacc tgatcttcaa tatgcaatcc
     6481 tgccgcaccg gctttaatca tcgatttcac ggtgcgcgcc acgttaaagg ccgaagaacc
     6541 aaaaccgata tccgcatcca ccagcagcgg cagcgaacaa acgtcggtaa tacggcgaat
     6601 gtcagtcagc acatcatcaa gagtagaaat accgagatcg ggcagcccca gcgatcctgc
     6661 cgccacgcca ccgccggaga gataaatcgc ctgatatccg gcacgctgcg ccagcagcgc
     6721 atggttagcg ttgatggtgc caacaatttg caacggggtt tctttgctaa gtgcagcgcg
     6781 aaacgcttta cctggagagt gtagagacat agcccatcct ttgttatcaa cttgttattt
     6841 gcgttgataa agacaaagca atgagtgtgc cacaattgcc ttaataaaat taaacattta
     6901 atttcaatga gttaaagata aagtcatgaa gtaatccgca aatatgcgtt tcagttaacg
     6961 tttcaggcaa tgtttcaccg cgtttcattg caacaattat gaaacaagac taaacccaat
     7021 attcggtttc ttaactttgc ggtgcgctat ggcacatcca ccacggctga atgacgacaa
     7081 accggttatc tggacagtat cggtgacgcg cctgttcgag ctgtttcgcg atatcagcct
     7141 cgagtttgat cacctggcga atatcacccc tattcagctt ggctttgaaa aagcggtgac
     7201 ctacatccgc aagaaactgg caaacgaacg ctgcgatgcc atcatcgctg ctggctctaa
     7261 cggcgcgtac ctgaaaagcc gcctgtcagt gccagttatt ttgattaaac cgagcggcta
     7321 cgatgtatta caggcactgg caaaagccgg aaaactcacc tcttctatcg gcgttgtcac
     7381 ttatcaggaa actattccgg cactggtggc gtttcaaaaa acctttaatt tgcgcctcga
     7441 ccaacgtagc tacattaccg aagaagacgc gcgcgggcag attaacgagc tgaaagctaa
     7501 cggcaccgaa gcggtggtcg gcgcggggct gattaccgat ctggcagaag aagccggaat
     7561 gaccggaatt tttatctatt ccgccgccac cgtacgccag gcattcagcg atgcgctgga
     7621 tatgacgcgc atgtcgttac gccataacac tcacgatgcc acccgcaacg ccctgcgtac
     7681 tcgttacgtg ctgggcgata tgctcggtca atcaccacag atggaacagg tacggcagac
     7741 tattttgctg tatgcccgct ccagtgcggc ggtgttgatt gagggggaaa cggggacggg
     7801 caaagagctg gcggcccagg cgattcatcg ggaatatttt gcccgccacg atgcgcgaca
     7861 gggcaaaaag tcgcatccgt ttgttgccgt caactgcggg gcgattgctg aatctctgct
     7921 ggaagcggag ctgtttggct atgaggaagg agcgtttacc ggctcgcgac gtggaggtcg
     7981 cgccgggctg ttcgaaattg cccacggcgg tacgctgttt ctggatgaga ttggcgaaat
     8041 gccgctacct ttgcagactc gcctgttacg ggtgctggaa gaaaaagagg tcacccgcgt
     8101 cggcgggcat cagcctgttc cggtggatgt gcgagtcatt agcgccactc actgcaatct
     8161 ggaagaagat atgcggcaag gggagtttcg ccgtgatctg ttttatcggc tgagtatttt
     8221 gcgtctgcaa ttgccaccac tgcgcgagcg ggtgacggat attctgccgc tggcggaaag
     8281 ctttttgaaa gtgtctctgg cggcgctctc cgccccgttt tctgccgcat tacgccaggg
     8341 gttacaggca agcgaaaccg tgctggtgca ctacgactgg ccgggcaata ttcgtgaact
     8401 gcgcaatatg atggagcgac tggcgctgtt tttaagtgtg gaaccgacgc cggatttaac
     8461 gccgcaattt ttgcagctgc tactgccgga actggcgcgc gagtcggcga aaactcccgc
     8521 tccacgctta ctgacaccac aacaggcact ggagaaattt aatggcgata aaacagcagc
     8581 ggcgaattat ttaggtatca gccggacgac gttctggcgg cggctgaaaa gctgaactgc
     8641 cgaatgcagc gcgaacgctt tatccggcat aaatttcaat agatgatgat caccgataaa
     8701 cttacgcatc ggggatgttt acataggatt cagaattact tcttcttata aatatttgcc
     8761 gtgccgtgga tcttattgtc agtttgaccg gaggtcagta ccaacacatc agcccctttt
     8821 tcatcggctt ttttgatcaa atcttctttc gcatctgcgg tcgacatttc attactggtt
     8881 gaaatatcac ctattttttc atattgcgat gcaacctttt caaattccgc tttggtcatt
     8941 aattctgcgg catagacatt ggtaacggca aacgctaacg caccgactaa cattttagag
     9001 attattttca tattacggct ccagagttgt tatcacacta tcacccaccc acattattgg
     9061 gttgattaat aattagttca gtcagggcat aaacgccagt aaccggaaat aagacacttt
     9121 gtgatctctc tcgcacccga ttatttttgg tagaaatatg atgaagttac ttcacttatt
     9181 tatggatgaa atcactatgg atcctttgca cgccgtttac ctgaccgtag gactgttcgt
     9241 gattactttt tttaatccgg gagccaatct ctttgtggta gtacaaacca gcctggcttc
     9301 cggtcgacgc gcaggggtgc tgaccgggct gggcgtggcg ctgggcgatg cattttattc
     9361 cgggttgggt ttgtttggtc ttgcaacgct aattacgcag tgtgaggaga ttttttcgct
     9421 tatcagaatc gtcggcggcg cttatctctt atggtttgcg tggtgcagca tgcgccgcca
     9481 gtcaacaccg caaatgagca cactacaaca accgattaac gccccctggt atgtcttttt
     9541 tcgccgcgga ttaattaccg atctctctaa cccgcaaacc gtgttatttt ttatcagtat
     9601 tttctcagta acattaaatg ccgaaacacc aacatgggca cgtttaatgg cctgggcggg
     9661 gattgtgctc gcatcaatta tctggcgagt ctttcttagt caggcgtttt ctttgcccgc
     9721 agtgcgtcgt gcttatgggc gtatgcaacg cgttgccagt cggattattg gtgcaattat
     9781 tggtgtattc gcgctacgcc tgatttacga aggggtgacg cagcggtgag cagataattt
     9841 actctctacg taatcaacct gatttgccgg atcccgacca tcggcgatga tagcccccgt
     9901 aatgcgtggt ccttatttcc gataagttta cttgagtctt tagcttccca tttcagccca
     9961 ctctcccctg tgtgggcttt tttcattgct ctgacccgtc ctgaatagcc aaacaatggc
    10021 aatgtttcac tttgcaggga tgataataag gggatctgat tcagaagagt tatgaaaagt
    10081 tggaccgcca tcgaggcctc gaaccccgta ccatacaaca atcctggttg tcgtctgctc
    10141 ttcctgatga gctaatggcg gtttttgata tgagccttac agcatatttt ctgtcaggct
    10201 tgagtgcgat gcgtaagata cagaaaagcc cccacacaaa catgatggaa ttatgatttt
    10261 tttcatatga atgtcaaccc acacattcac aaaacatctg catgagatca tttttttata
    10321 caccgtatag ctgcgaaacc actgctgatg cttttttaag cgaagcgtcg ccgatcacaa
    10381 caaaatatcg gcgaaaaagg cggtggaatg ccatttggca tacggatata cagatgaaat
    10441 gatcacctgc tttgcgattc actctctggt ggaataacaa ctcgcaggtg gaaccctttc
    10501 tgaactaaag agtgtagcgg attttaacgt gcttatttac aggataatat ctgtcaccta
    10561 actcctgcac tctattagat atccattcat ttaatcgcgc atcatctctg tatgttttta
    10621 cgtccagaac agagagcaat aacctcacca gtgattcagg aaaaacaggg tagatatttt
    10681 ttaagtgtaa taataatcca tgcgcaagcg cctgcttaat gggcgcgtcg gtctccggga
    10741 tagacgacat ttcgcgaagc catttttcca catgcataca ctctttccct ttagggaaaa
    10801 gtctcagcaa aggcatatgg tgttcaagaa gaatacattt gcaatctgtt cgcactgaat
    10861 cgataaactg ctctaatttc ttaagcccgg taaagcgtcc agcctgcaca tttgctttaa
    10921 tatcaagaaa cgcatcatcc tggtcatata cccaaactgt ccctttttga ttttcaagta
    10981 tgcgaaaaac aaacttaacc gggtgatcag ggtaatccat ccagtcataa aacccaggga
    11041 aatcattaaa ctgcacattt atggtgagtg cgttatcgtc tgaccactca cagtctcgta
    11101 cggcagccat gagatgaatg agtttgtcgt catcgactaa atcatcacga caatcataag
    11161 gataatcata atattgttta atcaaaacct tgaatatatc gacacaggag accagggagc
    11221 ttttgatatc ctctgataac tgattgtttt taacagtatt aagtatgctt tgttgcgtat
    11281 atggcatcaa attattgtgc ggagctttta atgatatcat cgcgatctct cttaaataaa
    11341 aataaacgtc gcagataata aagccctgat taaaccaggc actatcagaa atcgctcata
    11401 actaacgtgt gaagtattgt gtactggagg gagttaattt aacaatttga atttacgtta
    11461 taggttttac tgctactttg atattatttc gctctcctta actaaaaagt taagtggaga
    11521 gcgaagttaa tatgttaaac cacaggatat ttattaattg tttcagtctg ttagtgtgcg
    11581 attatcgata acaaaacgat atttcacatc acctcgcagc attcgctcat aggcttcatt
    11641 aatttgatcg gcccgaatca tctctatatc agccacgatg tcatgttcgg cgcaaaaatc
    11701 gagcatctcc tgtgtttctg gaatgccgcc aatcatagag ccggctatcg cacggcgttt
    11761 catgatcagg ttgaaaactt ccggtgattt atgcggtgtc gcaggcgcac caaccagcgt
    11821 catggtgcca tcacgcttca gcaaggtggt aaaatcgtcg agattatgtg gcgcagctac
    11881 tgtattcaaa atgaaatcga aactcttgag atgagccgcc atctcatcgg cattgcgtga
    11941 gttaacaact tcatcggccc ccagggcttt tgccgcttcg cgttttgcct cagaagtggt
    12001 aaatgccacc acatgcgccc ccatcgcgtg ggccagctta atccccatat gtcccagacc
    12061 gccgatgccg accacgccca ctttttttcc cggcccggcc tgccagtgac gtagcggcga
    12121 atacgtggtg atccctgcac acaacaaagg tgccaccgcc gccagctgct cttgcgggtg
    12181 acgaatacgc agaacatatc gctcatgaac gacgatctgt tgtgagtagc cgcccagagt
    12241 atggcccggt tcgtccggcg tcggcgagtt ataggtgccg gtcatgtgat cacagtagtt
    12301 ttccagcccg tcttcacact cttcgcaatg tttacaactg tcgacaatgc agccgacacc
    12361 gaccagatcg cccggcgcat gtttttctac ctgatcacca acggctacca cacgccccac
    12421 aatttcatga cccggcacac aggggtaaac cgtccccgcc cactcggaac ggacctggtg
    12481 gatatcggaa tggcagacgc cacagtaagc gatttcgatt ttgacatcat gcggtcccgg
    12541 ttcacgccgg gtgatatcca tcggttccag cggttgttta gcggaatatg caccaacagc
    12601 tttgatcttc attgtgttta ctcctgatta gctatgtgta tttggcgatg cctgatatgt
    12661 catcgcggca aaacgcgtcc attgaatata gccaatattt aaaccacccg atccagcccc
    12721 aacatgccgc gagcctgaat gaaacagagc agcaacgttc cccataaggc gagcgcctat
    12781 aagtgtgata tttgctatcc gtaaaagaca aaaatcggca aaactgatga tattttcacg
    12841 tagacaaata gttatctaca caacgtcaat aagggcatat tcttcaaaga taatgtccat
    12901 ttctgccgac acactcatac tcaggccgcc ccactccctg gggcttaaat ctcttactgt
    12961 tcaccgttgc agatatatca agttcgtatc aacctgccac actcccccac accagttgcc
    13021 ctttatggaa cgttgcggtt ctcggcgaga tacgcgccac cgcctcggcg gaacaggagg
    13081 cgtccaccag cacaaagctg gcgtcatcct gcgctttggg ccacacgcgc tcgccttttt
    13141 cgttgagcgg caatacatcg ccagtggcta aaaacagcga acgggacaaa ttctgttcgt
    13201 taggacgaat atagagctgc gcgtagagat tggctttttc cagcatgtcg cccaggccgt
    13261 agggagacca gtggtcgata acgctgtcgg tgccggtcat gacttttacg cctttgtcgt
    13321 gcaactgttt gagcggcata tgcagcgtgt caatcggcac cgtcgaggcg atagaaattt
    13381 gctgcgccgc catccggtgc gccagttcat ctacctgttg ttcgttgagc gttgccaacg
    13441 caaaggcgtg actgatggtc agcttgccct tcagctgtgg cgttttctct accgtttcaa
    13501 ccatataatt gatggctgcc acgcccgacg gagtggtttc gtgcaggtga atatcgacgc
    13561 ctttgtcgta gtccagcgca atctggaaca tggtgtcgag ggatttttcc atcgcgccat
    13621 caacactggt cgggtccagc ccgccgacgt aatgcgcccc cgcctgcatc gcttcgcgca
    13681 ttaaggcttc cgatttcgac agcagcaaac cgtgctgcgg gaaggcgaca atttcacact
    13741 cgaagcccgc ctgacgtcgc gccagcaccg cctgcaaatt ttgcagattt ttcaggccgg
    13801 aaaccggttc gatattgcaa tggctgcggg caatggtggt gcctttcgac tgcaataaat
    13861 cgatcagttt ttccacccgt tcctgcgtgt acggttgcag ttccggcagc attttctgct
    13921 cgagtttgat catgtcctgg atagtggtgc ctgccggacg attgagcgag cgccacggcc
    13981 cgccgtagaa ggttttgtcg agatgaatat gcatgtcgcg ggtggtgggc agcatcagct
    14041 taccgccagc gtcatagtgc ggtagcgtgg cgtccggatg ctgcttgttc tcgcgcaggg
    14101 caacaatttt accgtcctga atctccacgg tctggcgcgc cgtgcgggtc tgtaccgcca
    14161 cgccattttc gtagtcaaaa ccggtttcca gcagcacgtt atcgagatag taatgcgggt
    14221 cagtgatttt catggtgttg ttcgcttcgc agtttagggt gccagctacc cccgcatggg
    14281 cgagcggcac tgaggtacca aacagcgcgg cggcggtaac catcttaccg ctctggctca
    14341 gaaattcacg gcggctattg ttttctttca tcttacgtcc ttaggtttta ataatatggg
    14401 tgcccgtttc gccgcgtagc gccgcaggca ggcattccgg cgtggtgata atcacttctt
    14461 taccgccctg ctccaggaac gtcaggctgg cgatgatttt tggcaacatg ctgccgggtg
    14521 ggaaatgccc ttcctgcata tagcgggtca tggtggcaat atccacccga tccagcacct
    14581 gctgctgcgg tttgccaaag tgaatacaca ctttttccac gccagtggtg atcaccagaa
    14641 tgtcggcgtg aatttcacgg gccaatagcg cggtagagag atctttgtcg ataaccgcgt
    14701 ccacgctttg gtaatccccc gcttcagtac gcactaccgg aattccaccg ccgcccgcgc
    14761 caatcaccac aaaaccttgt tggatcagcg ccttaatggc aggtgcttcg acaatacgtt
    14821 tcggttccgg cgaggcgacc acgcggcgat agccccgtcc ggcatcttca acaaaacgcc
    14881 agtcagggtt tgccttttgt agttcgtcac gctggctttc actaaagaat gcgccgatgg
    14941 gcttggtggg atgggcaaac cccggatcgt ttttatccac ttccacctga gtcaccacgg
    15001 tgacggcttt tttctcgccg tgacgcgcca gccggttatt cagcgcctgc tggatcagat
    15061 agccaatgcc gccttgcgta tccgccacac agttcgccag cggcgttaag ggcagccctt
    15121 cgcgctcgtg ggcaatctcc gcgcggcgta aatctaaacc gacctgcggt ccgttgccgt
    15181 gggtcagcac aatgtcgtaa tcggaagcca gcatttccag caccgtatcg gcgacggcct
    15241 tcaccgcctc cgcctgatgc tcaatcgact ggctggcgtt atctttgata atgctgttgc
    15301 caccaatggc aacgaccaca agttctttca tgttgttatg tccgctacag agggtgatgg
    15361 ggttatgaga gtgaggttgc ccttctcccc aaaagggaga aggtatcgta aggtgcgttt
    15421 gcaggtcaga cgccgtaacg ttcgcaccag ccaagaatgg ctttttcgaa gcaggcgaat
    15481 ggtggatgca caatgcccgc gccaatcatg ccgacgcccg catctttatg ggcaatggcg
    15541 gtgttgatca ccggcagaat gccgctgcta cccacgcggg tgatgtcgat tgccgacggc
    15601 acgcccataa aaccgagcag cggaatggtg acgttggggt tttcgccgag agtgatttcg
    15661 cgcatctgac gggagaaatc gatagcctct tccaccgtac cgcccaccag cgcgacgatt
    15721 gcaggcgcgg tcgccatagc aaatccgcca atgccgtagg tttcggtgat ggcgctgtcg
    15781 ccgatatcca gccccgaatc ttccggctta taaccggcaa acatcgggcc gatcacctgc
    15841 tgcgccggac cggtaaacca ttgccccggt aatccgctga cccgcagacc gaactcgacg
    15901 ccgttacgcg ccatggtggt caccacagtg ctgtattcga tgccgtgcgc cgcatccatc
    15961 gccgctttac acatcgccat ccacgtcggg ccggagaagt agtcactgct ggcgacaaac
    16021 tcaaacactt cgcgctgttg ctcgacggag tagcccgcct gaataatccc cggcgtcagc
    16081 gcctgaatca gcagcgtcgt cccggcgtta ttgcggttat ggcactcatc gcccatatgc
    16141 agcgcctgcg ccagcattaa gcgcagatcg atttcgccga taattttcat cgcgtcgcgc
    16201 agcatgggtc cctgcacatc gcgcatccag ttgaggcggt cgatcacgct ctggtcgtta
    16261 gcgcccatac gcaaaatctt cgccatctgc tcgctcatgt tggtataagc gatgttgcca
    16321 taggttttgt ttttcacgat gtgcataaac atcgaggccg aggtaacacc cgccatcgat
    16381 cccacacagt catgttcgtg gcacggcgag aaagtgatct caccggaagc cgccagttca
    16441 gccgcctcgt cgagatcttt cgccagtcct tcaaacacca gcgctccggt gaccgcgcct
    16501 ttcatcgcgc cgcacatttt ttcccaggtg accggcggcc cggcgtgaag aatggttttc
    16561 ggcgtcatgc ccggcaccac gttaatcgcc tgatcaaaac caatcagcac cggatgtgac
    16621 tgaataatgc gctccggcgc ctgctgattc gcggcggcga ttttgtctgc cagcggcgaa
    16681 tcagcaatgt tatccagcgc ctgcaccacc tgcatattgc cctgccccgg cggcgtccag
    16741 tcgagttgag tcacttctac atgctgcttt ttcaggtcat cgctaaacat ggcgatgccg
    16801 acgttaataa cgttcaatgg ttggctaaac agtgactggc tcatcaggct tcctcccctt
    16861 tgcagataaa ttcacgcgcc agcaatccgg tattggtgct gctgctcgcc agaatcactc
    16921 cggcatcaag cagcatctgg ctttgttgtt ctaatgatgg cgtatcaaga tcggtaccca
    16981 gcacataggc gagaatgatc aactcacgtc cttcggcagc ggcgatcgct ttcgcctctt
    17041 tgatcgcctc gatggtggaa ccgaccggat cttcatgcga tccaaacccg agcacaaaat
    17101 ccatcacgat caacgccact tctggatcgc gcgcctcttc gatcaagcgg ctgatacggt
    17161 tggtggggtc aatcattgga tgcggcttgc cgttggtaaa atcgtcatcg ccaaagtcga
    17221 ggaaggtatg tttgacgctg cggttgatat ctttcaggcg gaattcagga tcgggctgga
    17281 tgttgctgta gacatcgccg tgtttttcca tcaccgcgaa catggtttcg tcacacagcg
    17341 tacctccgca gaacaggcca cgaatgtatt tctgtttcgg cttcaggcga gcgcgcacgt
    17401 cagcaatcaa tggctggttc agggtatgca gatcgagatg ttcctgtttc acgccggaga
    17461 gcattaccgc tttcagcgcc gcctctttgc tgccgcgagc aaactgtagc ccttgctcat
    17521 ccactggcgt ttcgccacga ccgaggaagc agaccaccac cggcttgcgg caggcacgtg
    17581 cgcgttccag cactttgcgg gccaccgcag gcgcaggcgg tttggagata agcacaatga
    17641 tttcagtttg cggatcgttt tccagcatcc cgatggcgtc gaacatcatc aggccgccga
    17701 ttttctcgct caggtcgcgc ccaccggtgc caatcagttg cgaaacgccg ccgccaaatt
    17761 catggatacg gacgctcagt tcctggctac cggtgccgga tgcgccaaca ataccgatgt
    17821 tgccgcgacg cacggcgtta ccaaagcaga gcgccgcgcc gttgataatc gccgtgccgc
    17881 agtctggccc catcagcagc agcccttttt catgggccaa ctgtttgagc gccagttcat
    17941 cttcaaccga gacgttatcg gaaaacagca tcacgttaag atcattttgc agcgcctgac
    18001 gcgcttcgcg agcggcaaac agaccgttga ccgaaatcac tgccaggtta ctttccggga
    18061 tatgcttttt ggcgctggca atagtggcgt aacgcgcctc gtgcgagccg ctttgcgctt
    18121 tggtgttgaa cagttcttca atctccacca gcaactgctc gttgtctgcg cccgctttgc
    18181 cgttgatgac aatcatcagg tcgccgtttt tcgcctgctc cagctccggc gtcagcagcc
    18241 ctaaattctt cagcacgcct ttgttcattt cggtcgccat cgccacaaat gcctgctcga
    18301 cgccgtcgag tttatttgca cgtgtggaga tagacatcag cgagacagaa tcaaaatagg
    18361 tattcggttt aatgactatt ttaactgaca taattccctc cgtaggtttg gctcagcgca
    18421 caaccatcgg ccatgccata cagcatgtcg cagccggaac tggagccaat atgtttaatt
    18481 ttttctattg cctgagttgc gttcccaggg atgtcataaa taatgttatg aataaaacga
    18541 tgaatatttt cccggcagcg ttgttgtaat gcggcttcca gcgttatggc acttaataac
    18601 gtggtattat ttctgccgcg ttgcagaccg agataaaatt cctctttgta tttttccgcc
    18661 ggatgtccgg aaataaataa aacaagcgcc agacctgtta aataatcgtc ggctgatggc
    18721 gtaaggccaa tccctaagcc catcatactg gcgactgctg aggcgatatt ttgtttatcg
    18781 tttacggcct gaataactgc ccgtcgttta atatgtaatt ctttatttaa cgcctgataa
    18841 aacggactat cgccgcgata attaaacagg gtttcatttt cccccaggtg ctgatgaatt
    18901 atgtcgtgcc actgttgcca ggcgataaag tgaaaattca cagcggtcat ttgcaaggtt
    18961 ggggacagcc aaagccgaca acgactcatc tctatatgaa gatgttgacc aaccgtaatg
    19021 ccttgatcgt caaactgaac cttatcacca tgccggaaca gaccatcaaa gtgagtgagt
    19081 gccaaccgac aactgtttgg cgcattgtcg tactcttcac aaagcagcgt tagcaactgc
    19141 tggcgagccg ggagtgcgat atttaccgct ctggcaaaga gctgttcaac ctgtccttgc
    19201 ccccgctgcg ccagaaaatc cgcatccgca gttagcgccc acacacaccg tctttctggt
    19261 gtgttgatag caagcctccc ggtttatgcg cctgcggcaa tcagtaactg cgcaatctct
    19321 tcaaagcctc gttcccgcgc cagttccagc ggcgttttgc catatttatc cgtcatgtgc
    19381 gggctggcac cgtgttccag caataactgg acaatcgcct gctgtttaat cccgccatca
    19441 ttaagcacaa ttgcttccag tagcggcgtc cagccaacat ggttggtctg gttaacgtta
    19501 atctccgtat gcgccaaaag ctcttttaca atacttaaat ggcctttttc acaggcaggc
    19561 gtcaggccga caccgccaaa acgggttacg caattaagat cgggtttagc cggtaaaata
    19621 atccgtaaca gggttaaatc gccggtcagg cagctaatta aaaaaggatt tgaacatatc
    19681 tgatcttgct tattaatatc cgctccggcg tcaattaatg cctgaacgca agcatattgc
    19741 tgatataaac ttgccagcgt aattgccgtt ttcccctgac gatcgcaggt attaatatcg
    19801 acacccagcg caaggcaggt ttttacttta tcgatatcac cctgttgcgc agccaataaa
    19861 tagtcggcag gtagattttt tatagtcatt cacattcccc tggtttttgt ggctgttttc
    19921 gatgaattga gaatagcaac gataatctga taaaagaacc cgcttaaatt taattcgccg
    19981 tatttcgcct ttttgttgaa tgatgttcaa caatcttttc gcagtgtgcg gtaatgcaat
    20041 atttgtttct ttgacccgat accctttcac tcaatcaggt tttatttctc tatagtctgc
    20101 acatgtaaga gtagctttcg tccatgtcta accagagggg aacaagatga acggactcac
    20161 ggcaacgggt gtcaccgtcg gtatttgtgc agggctttgg cagttagtgt cttcacatgt
    20221 cgggctctct cagggatggg aattattagg aaccattggc ttcgtcgcct tttgcagttt
    20281 ttatgccgca ggcggcggta aatcggggtt tatcagaagc ctggcggtta attattccgg
    20341 gatggtgtgg gcgtttttcg ccgcgctggc tgccggttgg ctggcctcgg tgagtggatt
    20401 gtccacgttc tgggcgagcg tgattatgac tgtacccttc tcggcggtgg tggtctggca
    20461 aggacgtttc ggtttattgt cttttattcc cggaggcttt ctcggcatga cgctattttt
    20521 tgccagcggg atgaactgga cggtgacgtt actgggtttt ctggcgggta actgcgtggg
    20581 gataatttca gaatatggcg gacaaaaatt aagcgaagcg acgactaaac gcgacgggta
    20641 ttaaaagtta ttcaaaaatg attatctatc tttaaccgat acctctacag tcatggctgg
    20701 agaaattcat attcgataat ggcgggaatg gtttaatgaa ctcaattttt accgaagaga
    20761 atctgttggc atttactacc gctgcgcgtt ttggcagttt cagtaaagca gcagaagagt
    20821 tgggtttaac cacttccgcc attagctaca ccatcaagcg aatggaaaca gggctggatg
    20881 tggtgctgtt cactcgcagt acccgaagca ttgagttaac ggagtccggg cgttattttt
    20941 tccgcaaagc taccgacctg ctgaatgatt ttcacgccat caaacgcagc atcgatacta
    21001 tttcgcaggg tatagaagca cgggtgcgca tttgtattaa ccaactgctt tatacgccca
    21061 aacacaccgc acgcttatta caggtactga aaaagcagtt tccaacctgc cagattaccg
    21121 tgacgaccga agtgtataac ggcgtctggg atgcgattat taataatcag gcgaatattg
    21181 ctatcggcgc gcccgacaca ctgctggacg gcggcggcat tgattacacg gagattggcg
    21241 cgattcgctg ggcttttgct atcgccccgg atcacccgtt agcctttgtg ccggaaccca
    21301 tcgccgaaag ccagctgcgt ctctatccta atatcatggt ggaagacacc gcgcatacga
    21361 ttaataaaaa agtgggctgg ttgctgcatg ggcaggagtc gattctggtg ccagatttca
    21421 acaccaaatg tcagtgccag attttaggtg aaggaattgg ttttttgccg gattacatgg
    21481 tccgtgaggc gatggcgcaa tccctgctgg ttacccgaca aatccataat ccgcgccagg
    21541 attcacgcat gttactcgca actcagcatt ctgctacggg tcaggtgacg cagtggatta
    21601 aaaagcaatt tgcgccaaat ggcatattaa cggggatcta tcaggattta ctgcatcggg
    21661 agaattaatc gctgccaacc tcccgatgcg ctgtgaatag cggtttacat caaccacctg
    21721 ctttcattac ccattcagag ataaatttat tacccggtac tggcggagag tacaaatagc
    21781 cctgcaaaaa gtgaactcct ttgccgatca ttaaatccgc ctgctcctgg gtttctaccc
    21841 cttccgccac gatactcaga ccaggcttac gcgccagttc gacaatattg tccacaatat
    21901 gaccggatat ttcgtccacg ctcgccattt gcacaaatga cttatcgatc ttaataaaat
    21961 cgaccgggaa cgcctgcaag taacgatagg tcgcataacc cgtaccaaag tcatccagcg
    22021 caaaggtaat gttgtgctga tgaaggctgt caaatatcgc tctggcttct ggcgttaccg
    22081 gaatagggtt acgttccgtc agctcaagaa ccagtttgat tttatcgtta cctaatttct
    22141 taaccaggtt cagacactct ttttcaaatc ctgcggccaa aaaacaaccc gccgagacgt
    22201 tgatgccaat atggaaattg tccggcagca aatgttttac cggcatcaga atatccgcag
    22261 tctgtttcat caactggcga gtcattatga cgataagacc agatgactcc gccagaggaa
    22321 taaactgatc cggtgggata attcccgttt gtggatgttc ccagcggaca agcacctcac
    22381 agcccgtcag taccccagtc tgcgcgcaga aaaccggttg gatccacggt ttaaattcat
    22441 ggttttccaa cgccagactg atggcttcag gcgtgacgat atggtgatag tgattatcat
    22501 taatatattt gtgactatta ttacccgtgg cggatatgat ctccggattg tactgaaaga
    22561 aaatatcccg ccagaaagta atatcgctct gaatccccag tttctcaaag agctgtttct
    22621 tttgatgtga aatggtcttt gcactgcgat ttctgtactg tgatatttct gttacagaca
    22681 ttccctgcat cagcagcttg agtagcagtc gttctttctc ggatacgcta ccaggcaatg
    22741 acaaatgaac cgttgtaccg aactcttgca gaaaaacacg aaaatcacat gaattcatga
    22801 acacaccttt tatcttttat cgctgttcag tatgagtgat tacctgtgga ttcctgagca
    22861 cacataaggg gtaaccataa acattagcga tgccatacgt ataacatccc acatcactat
    22921 caagaagatt atctttacct ctgatgctcg ctcaaaaaca gttacgctaa ataccttaaa
    22981 aaggtaatca caagtcaaac tcaggagata atcacgcaag aatgcaatat taaaattcag
    23041 atactcattt ttggtaataa agaagtagtt ggactatttc gatatttata atttcccact
    23101 acgtattgac gcccattcat gagtaaaggc gcattatatg aatttaacaa tccagatcaa
    23161 ctgaaaatac ctcaacctca taaacacata gcgtcaacat tcaatgacat aatgagtaaa
    23221 gatgttggtt atgcatacac gtcattactc tatgcctatc ccttaacaac ctgcttttta
    23281 agactgaatc cattcagcaa tagatagcca acattataac aatgacaata aaaacaatat
    23341 ttaaaacaat caataaaata tcagacacag acaattaaac cttaaacaac aaacggttac
    23401 attgatttta gttgatttgc gctacgcgtt ccccatgaat aaagcgtcat ttcaaattag
    23461 caccaatatt tacattaaat aagactaagc ttattgaaca ttcacaacag tccaaacgta
    23521 ggttttgggc ttcaatagtt cttttctttt ttattatctg ttgcctggca atcatcaatc
    23581 gatactaagg gcttaactgt acagatataa cgtatcaaga ggtaagagaa accgcgccat
    23641 atgcagacat ggcgcggttt tatgcaaaaa caatcacgcg tccgggaaca tcacgctatg
    23701 gcccggcgct tcacgatgga gatggataaa gttaagatgc cgctcgtact ggtcaagaat
    23761 atcggtgatc acctgctctt tgctgtagtc catcaggtcg ttgccctggc tgccttctaa
    23821 caggaaggtt tccagccggt agtaggtcga tttaccgcta cgtgcgcggt aggtaaagcc
    23881 cggcaccgaa tattgctgcg gccaaatctg atagacaaag ttttgctctt cgcccatatg
    23941 caccaacaaa tccagatgcc ccaactgttg tccctcttcc ggcggcaggc tttttagctc
    24001 cacgtacgcg ccgcgcaacc gcaactcctg cgccacttct tccattgccg ggtaacagac
    24061 cgtctccatc atctgtttag tgtaacgcgt gcccggataa ttcatcaggc gcgagagacg
    24121 ttttttccag ctcaggcgat cctgaagccc cagcggtcgc ggtgcggtat cgcggttggc
    24181 actttcacgg cggtaatctt ctaccttcag agatttatac aaccccgcca tcacgaagaa
    24241 gatcacaaag ctgaacggca ggcccataat caccgtggtg ttttgcagcg cggatatccc
    24301 gttggtcatc agcatgccga gcgtcagcag gccaatcgcc accgaccaga agacgcgcag
    24361 ccagccgggg gcgtcgctgt tgatatcttt aagctgcgag gtgaaattcc ccagcaccag
    24421 cgccccggag tccgccgagg tcacataaaa cagcaggcca gtaatggtgg cgacggaggc
    24481 gctaaaggta aacgccggat actgcgccag caggctgtag aagccgcgct ccggatggac
    24541 catcgcttcc tcggcaaatg ccgcgccgcc gtggatgatt tcatacagcg cgctattgcc
    24601 gaacaccgag agccataaca gcgtgaaggt aaacggaata atcaacgtgc ccagcacgaa
    24661 ctggcgaatg gtacgcccac gcgagatacg cgccaggaac aagccaacaa acggcgacca
    24721 tgccacccac catgcccaga agaagagcgt ccagttattc atccactcaa ccggacggtc
    24781 gaaggcaaaa ctgttgagcg tcatgcccat aaagcgattc atatagtcgc caacgttcag
    24841 caccagcgca ttaagcagga acgaggtgtc gcccataaac agcacgaaaa ggatcaatcc
    24901 cagcgccagc gcaacgttaa gttccgataa cacacgaatc cccttatcga caccggaggt
    24961 gacagagatc gtggcgatga tcaccgacaa ggcgatcagt gccgctttcg ccgccattga
    25021 atcgggaata tcaaacagta cgctcaagcc atagttaagc tgcaccacac cgataccgag
    25081 cgtagtggca ataccgaaga tggtgccgat caccgctgca atatccactg agtgacctat
    25141 cggcccgtta atccgtttac cgaagatcgg gtacagcgcc gagcggatgg tgagcggcaa
    25201 attataacga taactaaagt atccgagcgc catgcccatc agcgcataca tcgaccagcc
    25261 ggttaagccg tagtgaaaca gcgtccagac catcgcctga cgcgcggcct caatcgtctg
    25321 acccgcgcct tccggcggct gcatatactg cgttaccggt tcggctacgg agaagaacat
    25381 caggtcgata ccgatcccgg cagcaaacag catcgccgcc caactcagca gactgaattc
    25441 cggttttgat tgttctggcc cgagcttcac cgaaccaaaa cgcgaacaag cgatgcagac
    25501 cacaaagaca atatagagcg ttgccgccag cagattgtac caaccgaagg ttttagagac
    25561 ccagtccagc gtgcgtccaa tccacagggc cgagaagtcg cgaaacagga tcgttgtcag
    25621 ggaaaacaac aaaatcagtc cggcggaagt gtaaaacacc accggattga ttttgtcctt
    25681 ttccctgctg tgtgaaaggt ctgtcatcca gtatccccac tgttattgtt accatttaaa
    25741 atcaaattcg taacaattaa gacacatttt atattgaacg tccaatcaat aaccgcttta
    25801 atagataaac aacgctgatg aatggagtgg cgaaaatgcc caaattgggg atgcagtcga
    25861 tccggcgcag acaactgatc gacgccacgc tggaagcaat aaatgaagtg ggcatgcacg
    25921 atgcaacgat cgcgcagatc gcccgccgtg caggcgtttc tacggggatc atcagccact
    25981 atttcaggga caaaaatggt ctgctggaag caaccatgcg cgatatcacc agtcagctgc
    26041 gtgacgcggt tttgaatcga ttacatgcac ttccgcaggg cagtgcagag cagcgattac
    26101 aggcgattgt tggcggaaac ttcgacgaaa cgcaggtgag cagtgcggcg atgaaagcct
    26161 ggctggcgtt ctgggccagc agtatgcatc agccgatgct ctatcgcttg cagcaggtca
    26221 gtagccgccg cttattgtcg aatctggtga gcgaatttca ccgcgagtta ccgcgccaac
    26281 aggcgcagga agcgggctac ggacttgccg cgctgattga cggattatgg ctgcgcgcgg
    26341 cactgagcgg caaaccgctg gataaacctc tcgcacactc gctgacccgt cactttatta
    26401 ctcagcattt acccaccgat taaccgagga gacgtgatgt cccgaatggc agaacagcag
    26461 ctttatatac atggtggtta tacatccgcc accagcggtc gcaccttcga gaccattaac
    26521 ccggccaacg gtaacgtgct ggcgaccgtg caggccgccg ggcgcgagga tgtcgatcgc
    26581 gccgtgaaga gtgcccaaca ggggcaaaaa atctgggcgg cgatgaccgc tatggaacgc
    26641 tcgcgtattc tgcgtcgggc cgttgatatt ctacgtgaac gcaatgacga actcgcaaaa
    26701 ctggaaaccc tcgacaccgg aaaagcatat tcggaaacct caaccgtcga tatcgttacc
    26761 ggtgcggacg tgctggagta ctacgccggg ctgatcccgg cgctggaagg cagccagatc
    26821 ccgttgcgtg agacgtcatt tgtttatacc cgccgcgaac cgctgggcgt agtggcaggg
    26881 attggcgcat ggaactaccc gattcagatt gccctgtgga aatccgcccc agcgctggcg
    26941 gcaggcaatg caatgatttt caaaccgagc gaagtcaccc cgcttaccgc gttaaagctg
    27001 gccgaaattt acagcgaagc gggcctgccg gacggcgtat ttaacgtgtt gccgggcgtg
    27061 ggcgcggaga ccgggcagta tctgaccgag catccgggca ttgccaaagt gtcatttacc
    27121 ggcggtgtcg ccagcggcaa aaaagtgatg gctaactcgg cggcttcctc cctgaaagaa
    27181 gtgaccatgg agctgggcgg taaatcaccg ctgatcgttt ttgacgatgc ggatctcgat
    27241 ctcgccgccg atatcgccat gatggcgaat ttcttcagct ccggtcaggt gtgtaccaac
    27301 ggcacccgcg tcttcgttcc ggcgaaatgc aaagccgcat ttgaacaaaa gattctggcg
    27361 cgcgttgagc gcattcgcgc gggcgacgtt ttcgatccgc aaactaactt tggcccgctg
    27421 gtcagcttcc cgcatcgcga taacgtgctg cgttacatcg ccaaaggcaa agaggaaggc
    27481 gcgcgcgtac tgtgcggcgg cgatgtactg aaaggcgatg gcttcgataa cggcgcatgg
    27541 gttgcaccga ccgtgttcac cgattgccgc gacgaaatga ccatcgtgcg cgaagagatc
    27601 ttcggtccgg tgatgtcgct tctcacttat gaatctgagg acgaagtgat tcgccgcgct
    27661 aacgataccg actacggtct ggcggcgggt atcgtgacgg cggacctgaa cctcgcgcat
    27721 cgcgtcattc atcagctgga agcgggtatt tgctggatca acacctgggg cgaatccccg
    27781 gcagagatgc ccgttggcgg ctacaaacac tccggcattg gtcgcgagaa cggcgtgatg
    27841 acgctccaga gttacaccca ggtgaagtcc atccaggttg agatggctaa attccagtcc
    27901 atattctaac caggaggttt atttgcaatt tgactacatc attattggtg ccggctcagc
    27961 cggcaacgtc ctcgctaccc gtctgactga agatcagaac acaaccgtgc tgctgcttga
    28021 agcaggcggc ccggactatc gctttgactt ccgcacccag atgcccgccg cgctggcgtt
    28081 cccgctacag ggcaaacgct acaactgggc ctatgagacg gaacccgaac cgtttatgaa
    28141 caaccgccgc atggagtgcg gacgcggcaa aggtctgggc ggttcatcgc tgatcaacgg
    28201 catgtgttac atccgtggta acgcgctgga tctcgataac tgggcgcaag aacccggtct
    28261 ggagaactgg agctacctcg actgtctgcc ctactaccgc aaggccgaga ctcgcgacgt
    28321 gggcgagaac gactaccacg gcggcgacgg cccggtgagc gtcaccacct ccaaacccgg
    28381 cgtcaatccg ctgtttgaag cgatgattga agcgggcgtg caggcgggct acccgcgcac
    28441 ggacgatctc aacggctatc agcaagaagg ttttggcccg atggatcgca ccgtcacgcc
    28501 gcatggccgt cgcgccagca ccgcgcgtgg ctatctcgat caggccaaat cgcgctctaa
    28561 cctgaccatt cgtactcacg ccatgaccga tcacatcatt tttgacggca aacgcgcggt
    28621 gggcgtcgag tggctggaag gcgacagcac cattccgacc cgcgcggcgg cgaataaaga
    28681 agtgctgtta tgtgcaggcg cgattgcttc accgcagatc ctgcaacgct ccggcgtcgg
    28741 caacgctgga ctgctggcgg agtttgatat tccgctggtg catgaattac ctggcgtcgg
    28801 cgaaaatctt caggatcacc tggagatgta tctgcaatat gagtgcaaag aaccggtttc
    28861 cctctaccct gccctgcagt ggtggaatca gccgaaaatc ggtgcggagt ggctgtttgg
    28921 cggcaccggc attggtgcca gcaaccactt tgaagcaggc ggatttattc gcagccgtga
    28981 ggaatttgcg tggccgaata ttcagtacca tttcctgcca gtggcgatta actataacgg
    29041 ctcgaacgca gtgaaagagc acggcttcca gtgccacgtc ggatcgatgc gctcgccaag
    29101 ccgtgggcat gtgcggatta aatcccgcgc cccgcaccag catccagcga ttctgtttaa
    29161 ctacatgtcg cacgagcagg actggcagga gttccgcgac gcaattcgca tcacccggga
    29221 gatcatgcat caaccggcgc tggatcagta tcgtggccgc gaaatcagcc ccggtgtcga
    29281 atgccagacg gatgagcagc tcgatgagtt tgtgcgtaat cacgccgaaa ccgccttcca
    29341 cccatgcggt acgtgcaaaa tggggtacga cgagatgtcc gttgtggatg gcgaaggccg
    29401 cgtgcacggg ctggaaggat tacgcgtagt cgatgcgtca attatgccgc agattatcac
    29461 cggcaatctg aacgccacga cgattatgat tggcgagaaa atggcggata tgattcgcgg
    29521 gaaggatgcg ttgccgagga gcacggcggg atattatgtg gcaaatggga tgccggtgag
    29581 agcgaaaaaa atgagtcgtg atgtgaactg actcaggaac ctatcagatt gagagttacc
    29641 gttctactat gaggaccgaa tccttatata aaacactgag gtaactctca tgcttcatat
    29701 ccagtattca tgatgctggc tttttgctga tatttaaccc gaacattcct gatacagact
    29761 attaattcat ttatctgcgg cagattttat tttctggata tattatgagt gaacaaatca
    29821 aacaggatat cgatctgatt gagattttat tttatctgaa gaaaaaggta gatgaatacc
    29881 aacaaaactc tgggcagtaa taaacagcat tctgatagag agtgcctgta taaagctgaa
    29941 gtgacaatga ccgggaaggg aacccgtttt caggaatgga tgcctgtctt tttcaattta
    30001 aatatcctgg cattagtaat gccaggatat ttacagaaga gcagcataac catgacggaa
    30061 cggggttaag tcaggttgtc ctaaacgcta ttttcaacct cgtatgcctt cttcaggttt
    30121 atgtccagac ttcatatctc tctcaacaat cctcaataat cagatacacc gctttcaccg
    30181 ggccatgaac tccgacgact ttgataagct caatatccgc cgttgaactg gggccgctaa
    30241 tgatgttaat gcaggaaggc attcgttcac cggcctgcgc tttctgatgc aatttttctg
    30301 cgagttgcgc tacacgcggc aggatagtgc ttttacgcag gataaaaaga gaagattccg
    30361 ggagcaggct caatgaacgc ccgcgcccgg cggcggaaaa aagaaccacg cctcccgatt
    30421 cggttaaacc atattcagca tatacgacgc ccactttagc ctgctctgct tgtgatatgt
    30481 tctcaatacc tttcgccgga tcccaaacaa cggcattgca ttcctgctgc aaacgttcgc
    30541 taatccccaa ttcctccagc ctcgtgtcac cgctaatcac gatcgactga tctcccagct
    30601 ctttacacag acgtattgca gcttctgcgg cctgcgcctc gctggtcagt tcacagcgcg
    30661 tcaacataac atcgctggca aactggataa acgcgtcaca gcgctgctgt tggttaagtt
    30721 cggtaagccg ctcgttagca tagttgttaa gcggcgcatc ttctgcttgc ggttcaagtc
    30781 gctgcgggcg acccagtgcc tgagcaacgt tattcaaaaa ttcgctccga ttatccattc
    30841 tttttctcct gcgcctgatg tttcttaaac caactacgga aactctctcc gtcagcttca
    30901 ggaagatcgc gtgcttccat ccagtcgcta atcgcgccaa atttgagtgg tgttttgccg
    30961 ccattgataa accagcttgc cgcatgagcg ccggccatca tcccgacttt ccacaatcct
    31021 ggatgactat tggcataagc gaacattttt atcgcccgtt gctctgcttt tgcggtgatc
    31081 cctttttcag ccatcacccg acgatgacgc aaaatcagtt ttgacagcgg aatacgcacc
    31141 ggacacacgc tgtcacaagc ggtgcataaa gagcaggcgt agggtaaatc tttaaaatct
    31201 ttatagccgc caagtagcgg agaaatcacc gcaccaattg gccctggata aatagagcca
    31261 tatccatgac cgccaatatg gcgatatgcc ggacaagtat tcatacaagc cccgcagcga
    31321 atacagcgca gcacatcccg aaattcagag gccagcacct cagaacgccc gttatcgaca
    31381 ataaccagat gaaactcttc aggaccatca acgtgccccg cttcgcgcgg tcctgtcagc
    31441 caggtgttgt atcccgtcaa acgtgcacca acggcactgc gcgccagcat ggtgatcaat
    31501 acatctacct cggcaaacgt gggggcaata cgctccattc ccatcactgc aatatgcgtt
    31561 ttaggcagcg tggtacacat tcgcgcatta ccttcattgg tcaccaggca taccgaaccg
    31621 gtctctgcca ccgcgaaatt acagccggta atacctattt cagcactgag gaaatcttcg
    31681 cggatttttt gccggatgaa taaggtcatt gcttcaggcg tttccgaccc ctcatagccc
    31741 agacgttcgt gtagcacccg acggatctga tggcgatctt tatgaattgc cgggaccaca
    31801 acatgagagg gcggatcttg atccagctgg agaatgtatt cacccagatc ggtttcaatc
    31861 acctgaatgc cagcatcctg caacacatga ttgacaccaa tctcttcggt caccatcgat
    31921 ttagatttca ccaccttccg ggcatttttg cgttgggcaa cctgtaaaat gtagcgggta
    31981 gcgtcttctt tggtttttgc aaaatagacg tgaccgccgt tttgcgtcac tttttctgag
    32041 agctggtaca gataagcgtc gagattactc agaacatgat cacgtatctg ggcggcccga
    32101 tcgcgccact cctcccagtg ccccaattca tcgaccattt tttgccgatt tgccccgata
    32161 cgctgctgcg cgtttgccac cgctttgcgc atgatcggat cttcaatttg ctgacggatg
    32221 cgtgtcttaa aatctgtatt actggttttg atcgacatct ttatatcctc agcggctcat
    32281 caacacttca gcaatatgca tcactttgac tttctgccct tcccgttgta atcgcccact
    32341 gatgttcagc aggcaactca cgtcagcacc tattaaatac tcagggcgga cttccatcag
    32401 gtgcgcaacc ttttcttgca ccatctcgcc ggatatttcg gccattttaa ccgagaacgt
    32461 gccgccaaat ccgcagcagg tatcctgttc agcaaaggtc aacagctcca gtccacgcac
    32521 atttttcagc agcgtaagtg gttcgtcctt cacccccagc ttacgggcca ggctacaaga
    32581 tgggtgatac accgctctcc cttgcaaact ggcacctaca tcgactaccc ctaatgtatt
    32641 aacaataaaa gaggtgagat cctgcatacg cgcggcaacc ttttcggcac gtgatgccca
    32701 ttcaggttca tccgccagat acgtcgggta actttttacg gcataggtgc aagagccagc
    32761 cggtgaaata atgggatcgt cgttatcctc cagtgcggcg atcagatttt tcatccctgg
    32821 aatcgcttct ttgatataac cactattgat cgcaggctga ccgcagcatc cctgtttctc
    32881 cgggaaattt acgcgacagc cgagtttttc cagtagcagc acggagtctc gtgccattct
    32941 tgatttcagg gcgtcaccaa tacaggtgac aaagaaattg acattcacaa ctaatactcc
    33001 attacttcat acccatttat gcccgatggt tccccccttc cgggggatca atcatcaata
    33061 aatatggcgt tcttttttaa ttggctagca acataccacc cctcatttgt atgacaaatg
    33121 ataatctttc atctaaactt caaatgcaat aactaatttt atttctttcg gcatcgctca
    33181 cattttatag ggaaatcgac gatcattttt ggcataagaa atccatttaa aaaggctaca
    33241 tcccttatac ctattcagag ctacttattc aggggttatt gagatataca ctgatggtgt
    33301 acaaaaaaat caccgacaat aatcacgtat caagtaatag tcgggatttt agttaattga
    33361 agcaatttag tttgcatttt tatttaaaaa ctgaactaga gtccatcaaa aaaatatata
    33421 aagcccaaaa ccaccgttgg aaatagaaat agtcgaaagg ctcacaactc ccggaatgcg
    33481 gattatcttt gccccgattt ctggcgattt tattcagggt gcaagctgtc tgaccctttc
    33541 ccgatattct cccggagtac aaccaaactc gcggacaaac gccttgtgaa aagatgattc
    33601 actggcatag cctactgact cagcgatcac cacaacaggg agcatttccc gggaaaacat
    33661 ctgggccgct atttgtagac gcaactttgt taatacagcc agcggcgtgg ttccggaaac
    33721 atcgcggaaa agctgggc
//
LOCUS       DAMZFT010000042        33707 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.042, whole genome shotgun sequence.
ACCESSION   DAMZFT010000042 DAMZFT010000000
VERSION     DAMZFT010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 33707)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33707)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33707
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.042"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..1308
                     /locus_tag="PN977_002088"
     CDS             <1..1308
                     /locus_tag="PN977_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catecholate siderophore receptor Fiu"
                     /protein_id="HDI6042117.1"
                     /translation="NTTRWSRVKQDYLMTAIMGGASNITQPTSDVNSWTWSRTANTKD
                     VSNKILTNQTNLTSTFYTGAIGHDVSTGVEFTRETQTNYGVNPVTLPAVNIYHPDSSI
                     HPGGLTRNGANANGQTDTFAIYAFDTLQITRDFELNGGIRLDNYHTEYDSATACGGSG
                     RGAITCPAGVAKGSPVTTVDTARSGNLVNWKAGALYHLTENGNVYINYAVSQQPPGGN
                     NFALAQSGSGNSANRTDFKPQKANTSEIGTKWQVLDKRLLLTAALFRTDIENEVEQND
                     DGTYSQYGKKRVEGYEISGAGNITPAWQVIGGYTQQKATIKNGKDVAQDGSSSLPYTP
                     EHAFTLWSQYQATDDISVGAGARYIGSMHKGSDGAVGTPAFTEGYWVADAKLGYRVNR
                     NLDFQLNVYNLFDTDYVASINKSGYRYHPGEPRTFLLTANMHF"
     gene            1350..2027
                     /gene="ybiX"
                     /locus_tag="PN977_002089"
     CDS             1350..2027
                     /gene="ybiX"
                     /locus_tag="PN977_002089"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKHD-type hydroxylase YbiX"
                     /protein_id="HDI6042118.1"
                     /translation="MMYHIPGVLSPQDVARFREQLEQAEWVDGRVTTGAQGAQVKNNQ
                     QVDTRSTLYAALQNEVLNAVNQHALFFAAALPRTLSTPLFNRYQNNETYGFHVDGAVR
                     SHPQNGWMRTDLSATLFLSDPESYDGGELVVNDTFGQHRVKLPAGDLVLYPSSSLHCV
                     TPVTRGVRVASFMWIQSMIRDDKKRAMLFELDNNIQSLKSHYGESEEILSLLNLYHNL
                     LREWSEI"
     gene            2101..2367
                     /gene="ybiI"
                     /locus_tag="PN977_002090"
     CDS             2101..2367
                     /gene="ybiI"
                     /locus_tag="PN977_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc finger protein YbiI"
                     /protein_id="HDI6042119.1"
                     /translation="MASGWANDDAVNEQINSTIEDAIARARGEIPRGESLYECEECGA
                     PIPQARREAIPGVRLCIHCQQDKDLQKSAYTGYNRRGSKDSQLR"
     gene            2632..2892
                     /locus_tag="PN977_002091"
     CDS             2632..2892
                     /locus_tag="PN977_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HDI6042120.1"
                     /translation="MKTINTVVAAMALSTLSFGVFAAEPVTTSQAQNMNKIGVVSADG
                     ASTLDALEAKLAEKAAAAGASGYSITSATNNNKLSGTAVIYK"
     gene            complement(3032..4117)
                     /gene="hcxB"
                     /locus_tag="PN977_002092"
     CDS             complement(3032..4117)
                     /gene="hcxB"
                     /locus_tag="PN977_002092"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcXB"
                     /protein_id="HDI6042121.1"
                     /translation="MESGHRFDAQTLHSFIQAVFRQMGSEEQEAKLVADHLIAANLAG
                     HDSHGIGMIPSYVRSWSQGHLQINHHAKVVKEAGAAVTLDGDRAFGQVAAHEAMALGI
                     EKAHQHGIAAVALHNSHHIGRIGYWAEQCAAAGFVSIHFVSVVGIPMVAPFHGRDSRF
                     GTNPFCVIFPRKDNFPLLLDYATSAIAFGKTRVAWHKGVPVPPGCLIDVNGVPTTNPA
                     VMQESPLGSLLTFAEHKGYALAAMCEILGGALSGGKTTHQETLQTSPDAILNCMTTII
                     INPELFGAPDCSAQTEAFAEWVKASPHDDDKPILLPGEWEVNTRRERQEQGIPLDAGS
                     WQAICDAARQIGMPEETLQAFCQQLAS"
     gene            complement(4258..5220)
                     /gene="ybiB"
                     /locus_tag="PN977_002093"
     CDS             complement(4258..5220)
                     /gene="ybiB"
                     /locus_tag="PN977_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein YbiB"
                     /protein_id="HDI6042122.1"
                     /translation="MDYRKIIKEIGRGKNHARDLDRDTARGLYAHMLNGEVPDLELGG
                     VLIALRIKGEGEAEMLGFYEAMQNHTIKLTPPADKPMPIVIPSYNGARKQANLTPLLA
                     ILLHKLGFPVVVHGVSEDPTRVLTETIFELMGITPTLHGGQAQAKLDEHQPVFMPVGA
                     FCPPLEKQLAMRWRMGVRNSAHTLAKLATPFAEGEALRLSSVSHPEYIGRVAKFFSDI
                     GGRALLMHGTEGEVYANPQRCPQINLIDREGMRVLYEKQDTAGSELLPQAKDPETTAQ
                     WIERCLAGSEPIPESLKIQMACCLVATGEAATISDGLARVNQAF"
     gene            complement(5248..7398)
                     /gene="dinG"
                     /locus_tag="PN977_002094"
     CDS             complement(5248..7398)
                     /gene="dinG"
                     /locus_tag="PN977_002094"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase DinG"
                     /protein_id="HDI6042123.1"
                     /translation="MALTAALKAQIAAWYKALQEQIPDFIPRAPQRQMIADVAKTLAG
                     EEGRHLAIEAPTGVGKTLSYLIPGIAIAREEQKTLVVSTANVALQDQIYSKDLPLLKK
                     IIPDLKFTAAFGRGRYVCPRNLTALASTEPTQQDLLAFLDDELTPNNQEEQKRCAKLK
                     GDLDTYKWDGLRDHTDIAIDDDLWRRLSTDKASCLNRNCYYYRECPFFVARREIQEAE
                     VVVANHALVMAAMESEAVLPDPKNLLLVLDEGHHLPDVARDALEMSAEITAPWYRLQL
                     DLFTKLVATCMEQFRPKTIPPLAIPERLNAHCEELYELIASLNNILNLYMPAGQEAEH
                     RFAMGELPDEVLEICQRLAKLTEMLRGLAELFLNDLSEKTGSHDIVRLHRLILQMNRA
                     LGMFEAQSKLWRLASLAQSSGAPVTKWATREEREGQLHLWFHCVGIRVSDQLERLLWR
                     SIPHIIVTSATLRSLNSFSRLQEMSGLKEKAGDRFVALDSPFNHCEQGKIVIPRMRVE
                     PSLDNEEQHIAEMAAFFRKQVESKKHLGMLVLFASGRAMQRFLDYVTDLRLMLLVQGD
                     QPRYRLVELHRKRVANGERSVLVGLQSFAEGLDLKGDLLSQVHIHKIAFPPIDSPVVI
                     TEGEWLKSLNRYPFEVQSLPSASFNLIQQVGRLIRSHGCWGEVVIYDKRLLTKNYGKR
                     LLDALPVFPIEQPEVPEGIVKKKEKTKSPRRRRR"
     gene            complement(7569..7850)
                     /locus_tag="PN977_002095"
     CDS             complement(7569..7850)
                     /locus_tag="PN977_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001235511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin, RelE/StbE family"
                     /protein_id="HDI6042124.1"
                     /translation="MRLEWKPMSLSDREQIMEYISKDNPLAAIALDNQFEAIAEQACE
                     RPEMYRAGRVAGTHEAVVRPHYVLIYQVEGDTLSVLRVLHTALQWPPAQ"
     gene            complement(7850..8290)
                     /locus_tag="PN977_002096"
     CDS             complement(7850..8290)
                     /locus_tag="PN977_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001522657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6042125.1"
                     /translation="MAPETIDHSTLHKLVESGVVDAAHVIGTQGGWSLTVKYGHTERP
                     LAAQRSRQIRLFKRLETVVNYLKDVGIARFEVDASHYDPDGQKKTTRPDRAEALKRAH
                     EAAAYDAWFREQVQAAIDDPRPALSHEEAKSLFAARKKALLKGD"
     gene            complement(8447..9811)
                     /gene="rhlE"
                     /locus_tag="PN977_002097"
     CDS             complement(8447..9811)
                     /gene="rhlE"
                     /locus_tag="PN977_002097"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlE"
                     /protein_id="HDI6042126.1"
                     /translation="MSFDSLGLSPDILRAVAEQGYREPTPIQQQAIPAVLEGRDLMAS
                     AQTGTGKTAGFTLPLLQHLITRQPHAKGRRPVRALILTPTRELAAQIGENVRDYSKYL
                     NIRSLVVFGGVSINPQMMKLRGGVDVLVATPGRLLDLEHQNAVKLDQVEILVLDEADR
                     MLDMGFIHDIRRVLTKLPAKRQNLLFSATFSDDIKALAEKLLHNPLEIEVARRNTASD
                     QVTQHVHFVDKKRKRELLSHMIGKGNWQQVLVFTRTKHGANHLAEQLNKDGIRSAAIH
                     GNKSQGARTRALADFKSGDIRVLVATDIAARGLDIEELPHVVNYELPNVPEDYVHRIG
                     RTGRAAATGEALSLVCVDEHKLLRDIEKLLKKEIPRIAIPGYEPDPSIKAEPIQNGRQ
                     QRGGGGRGQGGGRGQQQPRRGEGGAKSASAKPAEKTSRRLGDAKPAGEQQRRRRPRKP
                     AAAQ"
     gene            10040..10711
                     /gene="cecR"
                     /locus_tag="PN977_002098"
     CDS             10040..10711
                     /gene="cecR"
                     /locus_tag="PN977_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CecR"
                     /protein_id="HDI6042127.1"
                     /translation="MNNPAMTIKGEQAKKQLIAAALAQFGEYGMNATTREIAAQAGQN
                     IAAITYYFGSKEDLYLACAQWIADFIGEQFRPHAEEAERLFAQPQPDRAAIRELILRA
                     CRNMIKLLTQDDTVNLSKFISREQLSPTAAYHLVHEQVISPLHSHLTRLIAAWTGCDA
                     SDTRMILHTHALIGEILAFRLGKETILLRTGWTAFDEEKTELINQTVTCHIDLILQGL
                     SQRSL"
     gene            10714..11709
                     /gene="hlyD"
                     /locus_tag="PN977_002099"
     CDS             10714..11709
                     /gene="hlyD"
                     /locus_tag="PN977_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein HlyD"
                     /protein_id="HDI6042128.1"
                     /translation="MKKPVVIGLAVVVLAAVVAGGYWWYQSRQDNGLTLYGNVDIRTV
                     NLSFRVGGRVESLAVDEGDAIKAGQVLGELDHKPYEIALMQAKAGVSVAQAQYDLMLA
                     GYRDEEIAQAAAAVKQAQAAYDYAQNFYNRQQGLWKSRTISANDLENARSSRDQAQAT
                     LKSAQDKLRQYRSGNREQDIAQAKASLEQAQAQLAQAELNLQDSTLIAPSDGTLLTRA
                     VEPGTVLNEGGTVFTVSLTRPVWVRAYVDERNLDQAQPGRKVLLYTDGRPDKPYHGQI
                     GFVSPTAEFTPKTVETPDLRTDLVYRLRIVVTDADDALRQGMPVTVQFGDEAGHE"
     gene            11702..13438
                     /locus_tag="PN977_002100"
     CDS             11702..13438
                     /locus_tag="PN977_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415315.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDI6042129.1"
                     /translation="MNDAVITLNGLEKRFPGMDKPAVAPLDCTIHAGYVTGLVGPDGA
                     GKTTLMRMLAGLLKPDSGSATVIGFDPIKNDGALHAVLGYMPQKFGLYEDLTVMENLN
                     LYADLRSVTGEARKQTFARLLEFTSLGPFTGRLAGKLSGGMKQKLGLACTLVGEPKVL
                     LLDEPGVGVDPISRRELWQMVHELAGEGMLILWSTSYLDEAEQCRDVLLMNEGELLYQ
                     GEPTALTQTMAGRSFLMTSPHEGNRKLLQRALKLPQVSDGMIQGKSVRLILKKEATPD
                     DIRHADGMPEININETTPRFEDAFIDLLGGAGTSESPLGAILHTVEGTPGETVIEAKE
                     LTKKFGDFAATDHVNFAVKRGEIFGLLGPNGAGKSTTFKMMCGLLVPTSGQALVLGMD
                     LKESSGKARQHLGYMAQKFSLYGNLTVEQNLRFFSGVYGLRGRAQNEKISRMSEAFGL
                     KSIASHATDELPLGFKQRLALACSLMHEPDILFLDEPTSGVDPLTRREFWLHINSMVE
                     KGVTVMVTTHFMDEAEYCDRIGLVYRGKLIASGTPDDLKAQSANDEQPDPTMEQAFIQ
                     LIHDWDKEHGNE"
     gene            13431..14564
                     /locus_tag="PN977_002101"
     CDS             13431..14564
                     /locus_tag="PN977_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDI6042130.1"
                     /translation="MSNPILSWRRVRALCVKETRQIVRDPSSWLIAVVIPLLLLFIFG
                     YGINLDSSKLRVGILLEQRSEAALDFTHTMTGSPYIDATISDNRQELIAKMQAGKIRG
                     LVVIPVDFAEQMERASATAPIQVITDGSEPNTANFVQGYVEGIWQIWQMQRAEDNGQT
                     FEPLIDVQTRYWFNPAAISQHFIIPGAVTIIMTVIGAILTSLVVAREWERGTMEALLS
                     TEITRTELLLCKLIPYYFLGMLAMLLCMLVSVFILGVPYRGSLLILFFISSLFLLSTL
                     GMGLLISTITRNQFNAAQVALNAAFLPSIMLSGFIFQIDSMPAVIRAVTYIIPARYFV
                     STLQSLFLAGNIPVVLVVNVLFLIASAVMFIGLTWLKTKRRLD"
     gene            14575..15681
                     /locus_tag="PN977_002102"
     CDS             14575..15681
                     /locus_tag="PN977_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDI6042131.1"
                     /translation="MFHRLWTLIRKELQSLLREPQTRAILILPVLIQVILFPFAATLE
                     VTNATIAIYDEDNGEHSVELTQRFARASAFTHVLLLKSPQEIRPTIDTQKALLLVRFP
                     ADFSRKLDTFQTAPLQLILDGRNSNSAQIAANYLQQIVKNYQQELLEGKPKPNNSELV
                     VRNWYNPNLDYKWFVVPSLIAMITTIGVMIVTSLSVAREREQGTLDQLLVSPLTTWQI
                     FIGKAVPALIVATFQATIVLAIGIWAYQIPFAGSLALFYFTMVIYGLSLVGFGLLISS
                     HCSTQQQAFIGVFVFMMPAILLSGYVSPVENMPVWLQNLTWINPIRHFTDITKQIYLK
                     DASLDIVWNSLWPLLVITATTGSAAYAMFRRKVM"
     gene            complement(15643..16053)
                     /locus_tag="PN977_002103"
     CDS             complement(15643..16053)
                     /locus_tag="PN977_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhQ family protein"
                     /protein_id="HDI6042132.1"
                     /translation="MKWQQRVRVATGLSCWQIMLHLLVVALLVVGWMSKTLVHVGVGL
                     CALYCVTVVMMLVFQRHPEQRWREVADVLEELTTTWYFGAALIVLWLLSRVLENNFLL
                     AIAGLAILAGPAVVSLLAKDKKLHHLASKHRVRR"
     gene            16186..16947
                     /locus_tag="PN977_002104"
     CDS             16186..16947
                     /locus_tag="PN977_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HDI6042133.1"
                     /translation="MPDQTQQFSFKVLTINIHKGFTAFNRRFILPELRDAVRTVSADI
                     VCLQEVMGAHEVHPLHVENWPDTSHYEFLADTMWSDFAYGRNAVYPEGHHGNAVLSRY
                     PIEHYENRDVSVDGAEKRGVLYCRIVPPMTGKAIHVMCVHLGLREAHRQAQLAMLAEW
                     VNELPDGEPVLVAGDFNDWRQKANHPLKVQAGLDEIFTRAHGRPARTFPVQFPLLRLD
                     RIYVKNASASAPTALPLRTWRHLSDHAPLSAEIHL"
     gene            16944..18185
                     /gene="clsB"
                     /locus_tag="PN977_002105"
     CDS             16944..18185
                     /gene="clsB"
                     /locus_tag="PN977_002105"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsB"
                     /protein_id="HDI6042134.1"
                     /translation="MKCSWREGNKIQLLENGEQYYPAVFKAIGEAQERIILETFIWFE
                     DDVGKQLHAALLAAAQRGVKAEVLLDGYGSPDLSDEFVNELTAAGVVFRYYDPRPRLF
                     GMRTNVFRRMHRKIVVIDALIAFIGGLNYSAEHMSSYGPEAKQDYAVRLEGPIVEDIL
                     QFEQENLPGQSAARRWWRRHHKAEENRQPGEAQVLLVWRDNEEHRDDIERHYLKMLTQ
                     ARREVIIANAYFFPGYRFLHALRKAARRGVRIKLIIQGEPDMPIVRVGARLLYNYLVK
                     GGVQVFEYRRRPLHGKVALMDDHWATVGSSNLDPLSLSLNLEANVIIHDRHFNQTLRD
                     NLNGIIAADCQQVDETMLPKRTWWNLTKSVLAFHFLRHFPALVGWLPAHTPRLAQVDP
                     PAQPTMETQDRVETENTGVKP"
     gene            18185..19141
                     /locus_tag="PN977_002106"
     CDS             18185..19141
                     /locus_tag="PN977_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0104 family protein"
                     /protein_id="HDI6042135.1"
                     /translation="MSKSHPRWRLAKKLLTWLFFIAVIVLLVVYAKKVDWEEVWKVIR
                     DYNRVALLSAVGLVVVSYLIYGCYDLLARFYCGHKLAKRQVMLVSFICYAFNLTLSTW
                     VGGIGMRYRLYSRLGLPGSTITRIFSLSITTNWLGYILLAGIIFTAGVVELPDHWYVD
                     QTTLRILGIGLLMIIAVYLWFCAFAKHRHMTIKGQKLVLPSWKFALAQMLISSVNWMV
                     MGAIIWLLLGQSVNYFFVLGVLLVSSIAGVIVHIPAGIGVLEAVFIALLAGEHTSKGT
                     IIAALLAYRVLYYFIPLLLALVCYLVLESQAKKLRAKNEAAM"
     gene            complement(19177..19890)
                     /locus_tag="PN977_002107"
     CDS             complement(19177..19890)
                     /locus_tag="PN977_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HDI6042136.1"
                     /translation="MESYSQNSNKLDFQHEARILNGIWLITALGLVATAGLAWGAKYL
                     EITATKYDSPEMYIAIGLLLLSMYGLSKDINKINTAIAGVIYLFLISLVAIVVASLAP
                     VSAIIVVFSTAGAMFFISMLAGLLFNVDPGSHRFTIMMTLTGLALVIIVNATLMSERP
                     IWVISCLMIVLWSGIISHGRNKLLELAGKCHSEELWSPVRCAFIGALTLYYYFIGFFG
                     ILAAIAITLVWQRHTRFFH"
     gene            complement(20097..20801)
                     /locus_tag="PN977_002108"
     CDS             complement(20097..20801)
                     /locus_tag="PN977_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HDI6042137.1"
                     /translation="MDRFPRSDSIVQTRTGLQTYMAQVYGWMTVGLLLTAFVAWYAAN
                     SAAVMELLFTNRIFLIGLVIAQLALVIVLSAMIQKLSAGVTTMLFMLYSALTGLTLSS
                     IFIVYTAASIASTFVVTAGMFGAMSLYGYTTKRDLSGFGNMLFMALIGIVLASLVNFW
                     LKSEALMWAVTYIGVIVFVGLTAYDTQKLKNMGEQIDTRDTSNLRKYSILGALTLYLD
                     FINLFLMLLRIFGNRR"
     gene            complement(20938..21390)
                     /gene="moaE"
                     /locus_tag="PN977_002109"
     CDS             complement(20938..21390)
                     /gene="moaE"
                     /locus_tag="PN977_002109"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase catalytic subunit MoaE"
                     /protein_id="HDI6042138.1"
                     /translation="MAETKIVIGPQPFSVGEEYPWLAERDEDGAVVTFTGKVRNHNLG
                     DSVKALTLEHYPGMTEKALAEIVDEARNRWPLGRVTVIHRIGELWPGDEIVFVGVTSA
                     HRSSAFEAGQFIMDYLKTRAPFWKREATPEGDRWVEARESDQQAAKRW"
     gene            complement(21392..21637)
                     /gene="moaD"
                     /locus_tag="PN977_002110"
     CDS             complement(21392..21637)
                     /gene="moaD"
                     /locus_tag="PN977_002110"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase sulfur carrier subunit"
                     /protein_id="HDI6042139.1"
                     /translation="MIKVLFFAQVRELVGTDATEVAADFPTVEALRQHLAAQSDRWAL
                     ALEDGKLLAAVNQTLVSFDHPLTDGDEVAFFPPVTGG"
     gene            complement(21630..22115)
                     /gene="moaC"
                     /locus_tag="PN977_002111"
     CDS             complement(21630..22115)
                     /gene="moaC"
                     /locus_tag="PN977_002111"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HDI6042140.1"
                     /translation="MSQLTHINAAGEAHMVDVSAKAETVREARAEAFVTMRSETLAMI
                     IDGRHHKGDVFATARIAGIQAAKRTWDLIPLCHPLMLSKVEVNLQAEPEHNRVRIETL
                     CRLTGKTGVEMEALTAASVAALTIYDMCKAVQKDMVIGPVRLLAKSGGKSGDFKVEAD
                     D"
     gene            complement(22118..22630)
                     /gene="moaB"
                     /locus_tag="PN977_002112"
     CDS             complement(22118..22630)
                     /gene="moaB"
                     /locus_tag="PN977_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein B"
                     /protein_id="HDI6042141.1"
                     /translation="MSQVSTEFIPTRIAILTVSNRRGEEDDTSGHYLRDSAQEAGHHV
                     VDKAIVKENRYAIRAQVSAWIASDDVQVVLITGGTGLTEGDQAPEALLPLFDREVEGF
                     GEVFRMLSFEEIGTSTLQSRAVAGVANKTLIFAMPGSTKACRTAWENIIAPQLDARTR
                     PCNFHPHLKK"
     gene            complement(22652..23641)
                     /gene="moaA"
                     /locus_tag="PN977_002113"
     CDS             complement(22652..23641)
                     /gene="moaA"
                     /locus_tag="PN977_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HDI6042142.1"
                     /translation="MASQLTDAFARKFYYLRLSITDVCNFRCTYCLPDGYKPSGVTNK
                     GFLTVDEIRRVTRAFASLGTEKVRLTGGEPSLRRDFTDIIAAVRENDAIRQIAVTTNG
                     YRLERDVANWRDAGLTGINVSVDSLDARQFHAITGQDKFNQVMAGIDAAFEAGFEKVK
                     VNTVLMRDVNHHQLDTFLNWIQHRPIQLRFIELMETGEGSELFRKHHISGQVLRDELL
                     RRGWIHQLRQRSDGPAQVFCHPDYAGEIGLIMPYEKDFCATCNRLRVSSIGKLHLCLF
                     GEGGVNLRDLLEDDTQQQALEARISAALREKKQTHFLHQNNTGITQNLSYIGG"
     regulatory      complement(23629..23773)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01055"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="molybdenum cofactor riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="molybdenum cofactor"
                     /db_xref="RFAM:RF01055"
     gene            24038..24946
                     /gene="yvcK"
                     /locus_tag="PN977_002114"
     CDS             24038..24946
                     /gene="yvcK"
                     /locus_tag="PN977_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HDI6042143.1"
                     /translation="MRNRTLADLDRVVALGGGHGLGRVLSSLSSLGSRLTGIVTTTDN
                     GGSTGRIRRSEGGIAWGDMRNCLNQLITEPSVASAMFEYRFGGNGELSGHNLGNLMLK
                     ALDHLSVRPLEAINLIRNLLKVDAHLIPMSEHPVDLMAIDDQGHEVYGEVNIDQLTAP
                     IQELLLTPNVPATREAVHAISEADLIIIGPGSFYTSLMPILLLNEIAQALRRTPAPMV
                     YIGNLGRELSLPAANLKLESKLAIMEQYVGKKVIDAVVVGPKADVSAVNNRIVIQEVL
                     EASDIPYRHDRQLLHNALEKALQALG"
     gene            complement(24984..27005)
                     /gene="uvrB"
                     /locus_tag="PN977_002115"
     CDS             complement(24984..27005)
                     /gene="uvrB"
                     /locus_tag="PN977_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit B"
                     /protein_id="HDI6042144.1"
                     /translation="MSKPFKLNSAFKPSGDQPEAIRRLEEGLEDGLAHQTLLGVTGSG
                     KTFTIANVIADLQRPTMVLAPNKTLAAQLYGEMKEFFPENAVEYFVSYYDYYQPEAYV
                     PSSDTFIEKDASVNEHIEQMRLSATKAMLERRDVVVVASVSAIYGLGDPDLYLKMMLH
                     LTVGMIIDQRAILRRLAELQYARNDQAFQRGTFRVRGEVIDIFPAESDDIALRVELFD
                     EEVERLSLFDPLTGQIVSTIPRFTIYPKTHYVTPRERIVQAMEEIKEELAARRKVLLE
                     NNKLLEEQRLTQRTQFDLEMMNELGYCSGIENYSRFLSGRGPGEPPPTLFDYLPADGL
                     LVVDESHVTIPQIGGMYRGDRARKETLVEYGFRLPSALDNRPLKFEEFEALAPQTIYV
                     SATPGNYELEKSGGDVVDQVVRPTGLLDPIIEVRPVATQVDDLLSEIRQRAAINERVL
                     VTTLTKRMAEDLTEYLEEHGERVRYLHSDIDTVERMEIIRDLRLGEFDVLVGINLLRE
                     GLDMPEVSLVAILDADKEGFLRSERSLIQTIGRAARNVNGKAILYGDKITPSMAKAIG
                     ETERRREKQQKYNEEHGITPQGLNKKVVDILALGQNIAKTKAKGRGKSRPIVEPDNVP
                     MDMSPKALQQKIHELEGLMMQHAQNLEFEEAAQIRDQLHQLRELFIAAS"
     gene            complement(27584..28261)
                     /gene="bioD"
                     /locus_tag="PN977_002116"
     CDS             complement(27584..28261)
                     /gene="bioD"
                     /locus_tag="PN977_002116"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent dethiobiotin synthetase BioD"
                     /protein_id="HDI6042145.1"
                     /translation="MSKRYFVTGTDTEVGKTVASCALLQAAKAVGYRTAGYKPVASGS
                     EKIPEGLRNSDALALQRNSSLQLDYATVNPYTFAEPTSPHIISAQEGRPIESLVMSAG
                     LRALEQQADWVLVEGAGGWFTPLSDTFTFADWVTQEQLPVILVVGVKLGCINHAMLTA
                     QAIQHAGLTLAGWVANDVTPPGKRHAEYMTTLTRMIPAPLLGEIPWLAENPENAATGK
                     YINLALM"
     gene            complement(28254..29009)
                     /gene="bioC"
                     /locus_tag="PN977_002117"
     CDS             complement(28254..29009)
                     /gene="bioC"
                     /locus_tag="PN977_002117"
                     /EC_number="2.1.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-ACP O-methyltransferase BioC"
                     /protein_id="HDI6042146.1"
                     /translation="MTTVNKQAIAAAFGRAAAHYEQHADLQRQSADVLLAMLPQRKYT
                     HVLDAGCGPGWMSRHWRERHAQVTALDLSPPMLVQARQKDAADHYLAGDIESLPLATA
                     TFDLAWSNLAVQWCGNLSTALRELYRVVRPGGVVAFTTLVQGSLPELHQAWQAVDERP
                     HANRFLPPDEIEQSLNGLHYQHHIQPITLWFDDALSAMRSLKGIGATHLHEGRDPRIL
                     TRSQLQRLQLAWPQKQGRYPLTYHLFLGVIARE"
     gene            complement(28996..30150)
                     /gene="bioF"
                     /locus_tag="PN977_002118"
     CDS             complement(28996..30150)
                     /gene="bioF"
                     /locus_tag="PN977_002118"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HDI6042147.1"
                     /translation="MSWQEKINAALDARRAADALRRRYPVAQGAGRWLVADDRHYLNF
                     SSNDYLGLSHHPQIIRAWKLSAEQFGVGSGGSGHVSGYSVAHQALEEELAEWLGYSRA
                     LLFISGFAANQAVITAMMAKEDRIVADRLSHASLLEAASLSPSPLRRFAHNDVTHLAR
                     LLASPCPGQQLVVTEGVFSMDGDSAPLEEIQQVTQQHDGWLMVDDAHGTGVIGEQGRG
                     SCWLQKVKPELLVVTFGKGFGVSGAAVLCSNTVADYLLQFARHLIYSTSMPPAQAQAL
                     RASLAVIRSDEGDARREKLAALITRFRAGVQDLPFTLADSWSAIQPLIVGDNSRALQL
                     AEKLRQQGCWVTAIRPPTVPAGTARLRLTLTAAHEMQDIDRLLEVLHDNG"
     gene            complement(30147..31187)
                     /gene="bioB"
                     /locus_tag="PN977_002119"
     CDS             complement(30147..31187)
                     /gene="bioB"
                     /locus_tag="PN977_002119"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HDI6042148.1"
                     /translation="MAHRPRWTLSQVTELFEKPLLDLLFEAQQVHRQHFDPRQVQVST
                     LLSIKTGACPEDCKYCPQSSRYKTGLEAERLMEVEQVLESARKAKAAGSTRFCMGAAW
                     KNPHERDMPYLEQMVQGVKAMGLEACMTLGTLSESQAQRLANAGLDYYNHNLDTSPEF
                     YGNIITTRTYQERLDTLEKVRDAGIKVCSGGIVGLGETVKDRAGLLLQLANLPTPPES
                     VPINMLVKVKGTPLADNDDVDAFDFIRTIAVARIMMPTSYVRLSAGREQMNEQTQAMC
                     FMAGANSIFYGCKLLTTPNPEEDKDLQLFRKLGLNPQQTAVLAGDNEQQQRLEQALMT
                     PDTDEYYNAAAL"
     gene            31274..32563
                     /gene="bioA"
                     /locus_tag="PN977_002120"
     CDS             31274..32563
                     /gene="bioA"
                     /locus_tag="PN977_002120"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HDI6042149.1"
                     /translation="MTTDDLAFDQRHIWHPYTSMTSPLPVYPVASAEGCELILSDGRR
                     LVDGMSSWWAAIHGYNHPQLNAAMKSQIDAMSHVMFGGITHAPAIELCRKLVAMTPQP
                     LECVFLADSGSVAVEVAMKMALQYWQAKGEARQRFLTFRNGYHGDTFGAMSVCDPDNS
                     MHSLWKGYLPENLFAPAPQSRLDGEWDERDMVGFARLMAAHRHEIAAVIIEPIVQGAG
                     GMRIYHPEWLKRIRKMCDREGILLIADEIATGFGRTGKLFACEHAEIAPDILCLGKAL
                     TGGTMTLSATLTTREVAETISNGEAGCFMHGPTFMGNPLACAAANASLAILESGDWQH
                     QVAAIEGQLREQLAPACDAEMVADVRVLGAIGVVETTRPVNMAALQKFFVEQGVWIRP
                     FGKLIYLMPPYIILPQQLQRLTAAVNRAVQDETFFCQ"
     gene            32622..33098
                     /locus_tag="PN977_002121"
     CDS             32622..33098
                     /locus_tag="PN977_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase inhibitor"
                     /protein_id="HDI6042150.1"
                     /translation="MKLISNDLRDGDKLPHRHVFNGMGYDGDNISPHLAWDDVPVGTK
                     SFVVTCYDPDAPTGSGWWHWVVVNLPADTRVLPQGFGSGLVAMPDGVLQTRTDFGKTG
                     YDGAAPPKGETHRYIFTVHALDVERIDVDEGASGAMVGFNVHFHSLASASITAMFS"
BASE COUNT         7881 a         9066 c         8757 g         8003 t
ORIGIN      
        1 aatactaccc gttggtcgcg cgtgaagcag gattacctga tgacggcgat tatgggtggg
       61 gcgtcgaata ttacccagcc caccagcgat gtgaatagct ggacgtggtc acgcacggcg
      121 aataccaaag atgtgagtaa caaaattctc accaaccaga ccaacctgac ctcgacgttc
      181 tataccggcg ctatcggtca tgatgtcagt accggcgtgg aatttacccg tgaaacgcag
      241 actaactatg gcgttaatcc ggtgacgtta ccggcggtaa atatttatca tcctgacagc
      301 agcattcatc ccggcggcct gacgcgcaac ggcgcaaacg ccaatggtca gacggatacc
      361 ttcgcaattt acgccttcga tacgctgcaa atcacccgtg attttgagct gaacggcggg
      421 atccgtctgg ataattatca tactgaatat gacagtgcca ccgcctgcgg cggcagcgga
      481 cgcggtgcca tcacctgccc agctggtgtg gcaaaaggtt ctccggtcac caccgtcgac
      541 accgccaggt cgggcaatct ggtgaactgg aaagccgggg cgctgtatca cctgacggaa
      601 aacggcaatg tctatattaa ctatgccgtt tcccagcagc ctccgggcgg caacaacttc
      661 gcccttgcgc agtctggcag cggtaacagt gccaaccgca ccgattttaa accgcaaaaa
      721 gccaacacca gcgagattgg caccaaatgg caggttctgg ataaacgcct gttgctcacc
      781 gccgcgctgt tccgtactga tatcgaaaat gaagttgagc aaaatgatga cggaacttac
      841 tcgcaatacg gtaagaaacg cgtcgaaggc tatgagatat ccggggccgg gaatatcacc
      901 cccgcgtggc aggtgattgg cggctatacc cagcaaaaag caaccatcaa aaacggcaaa
      961 gatgttgccc aagatggttc ctcatcgctg ccctatacgc cggagcacgc ctttacctta
     1021 tggagccaat atcaggcaac cgatgatatt tccgttggtg cgggcgcacg gtatatcggc
     1081 agtatgcata aaggttctga cggcgcggtg ggaacgccag cgtttaccga aggttattgg
     1141 gtcgccgacg ctaaactggg gtatcgggtt aatcgcaatc tcgacttcca gctaaacgtc
     1201 tacaacctgt ttgataccga ttacgtcgcc tcaatcaaca agagcggcta ccgttatcac
     1261 ccgggcgagc caagaacctt cttgctcacg gccaatatgc atttctgatt cagatgtggg
     1321 gcgcaggccc cactttttgg agaacttgta tgatgtacca cattcccggc gtgctatcgc
     1381 cacaggacgt cgcccggttt cgcgaacaac tggaacaagc cgaatgggtg gatggacgcg
     1441 tcaccaccgg cgcacaaggt gcgcaagtta agaacaatca acaggtagac acccgcagca
     1501 cgttatacgc cgccctgcaa aatgaggtgc tgaacgcggt taatcaacat gctttattct
     1561 ttgccgcggc cttgccgcgt accctttcca cgccgctgtt taatcgctat cagaacaatg
     1621 aaacctatgg tttccatgtg gatggcgcag tacgcagcca tccacaaaac ggctggatgc
     1681 gcactgacct ttctgccacg ctgtttttaa gcgatccaga aagctacgac ggcggcgaac
     1741 tggtcgttaa tgacaccttc gggcaacatc gggtaaaact cccggcaggc gatctggtgt
     1801 tgtatccctc cagcagcctg cattgcgtga cacccgtaac ccgcggcgtg cgagtggcat
     1861 catttatgtg gatccagtcg atgatccgcg atgataaaaa gcgcgccatg ctgtttgaac
     1921 tggacaacaa tattcagtcg ctgaaaagcc actacggcga aagtgaagag atcctgtcgc
     1981 tgctcaatct ttatcataat ctgctgcggg aatggtcgga gatctgacgc ttgaactgcc
     2041 cgagttcgtc cctatattag ctacacttaa ctgtacaagc attgatatgg ggaggacgat
     2101 atggcatccg gttgggctaa cgatgacgcc gtcaacgaac agatcaacag tacaattgaa
     2161 gatgcgattg ctcgtgcccg gggtgaaatt ccgcgtggcg agagcctgta tgaatgtgaa
     2221 gagtgcggcg cccccatccc gcaggcccgt cgggaagcca ttcctggcgt gcgcttatgc
     2281 attcattgtc agcaggataa agatttacaa aaatcagctt atacaggata taatcgcaga
     2341 ggttcgaaag acagccagtt acgttaacta caactggcgg gatgtatagc gagtataatt
     2401 tgtgttttgc gtggcattcc gtgcctttaa cgctgtcgtg actgccacgc tgcataaaac
     2461 aagtccataa gttcaacaaa taaatggatt atcagtatgt tagatatcaa tcaatttatt
     2521 tgaacaaggc ggtcaattct cttcgatttt atctctcgta aaaaaacgtg atactcatca
     2581 catcgacgaa acaacgtcac ttatacaaaa atcacctgcg agagattaat tatgaaaact
     2641 atcaatactg ttgttgctgc tatggctctt tcaactctgt catttggcgt gttcgcggcg
     2701 gaaccggtaa cgacatccca ggcacagaac atgaataaaa tcggcgtggt ttctgccgat
     2761 ggcgcatcca ccctcgatgc cctggaagcg aaactggctg agaaagccgc ggcggccggt
     2821 gccagtggat acagcatcac ttctgccacc aacaacaata aattaagcgg tactgcggta
     2881 atttataagt aattcgtatg ctagatcagg cgtttacgct acattcagca caagtatttc
     2941 cctgtctgtt tgccgacaga cgcatatgct ctaaccctca ttgatcctat gttacccttg
     3001 tttgcccgtc cgccactgga cgggcttttt tttagctggc taactgctga cagaaagcct
     3061 gcaacgtctc ttccggcata ccaatctgcc gcgctgcatc acaaatggcc tgccagcttc
     3121 ccgcatccag tggaatcccc tgctcctgcc gttcgcgacg cgtgttcact tcccactcgc
     3181 ccggtagcaa aatcggctta tcgtcatcat gcggcgaggc tttcacccac tcggcaaagg
     3241 cttcggtctg tgcactacaa tccggcgcgc cgaacagttc cgggttgatg atgatagtgg
     3301 tcatgcagtt aagaatggca tcgggactgg tttgtaacgt ttcctgatgc gtcgttttac
     3361 cgccagaaag cgccccgcca agaatttcac acatcgctgc aagggcgtag cctttatgtt
     3421 cggcaaaggt caacagcgaa cccaacggcg actcctgcat taccgccgga tttgtcgtcg
     3481 gcacgccgtt aacgtcaatc aggcaacctg gcggcacggg gacgccttta tgccaggcga
     3541 cgcgggtttt gccaaatgca atggcgctgg tggcgtaatc gagcaacagc ggaaaattat
     3601 ctttacgagg gaaaatcaca cagaacggat tggtgccaaa gcggctgtcg cgaccgtgga
     3661 acggcgcgac cattggaata ccgaccacgc taacaaagtg gatagagaca aaccccgccg
     3721 ctgcacactg ctccgcccag taaccgatac ggccgatatg atgcgagtta tggagcgcca
     3781 cggcggcaat gccgtgctga tgcgctttct caatccccag cgccatcgct tcgtgtgccg
     3841 cgacctgacc aaatgcgcga tcgccatcga gcgtgaccgc cgcccccgcc tctttaacga
     3901 ctttggcatg atggttaatt tgcagatgcc cctgactcca ggagcgcaca tagcttggga
     3961 tcatgccaat accatgtgaa tcatgccctg ccaggtttgc cgcgattaaa tgatcggcaa
     4021 ctaatttcgc ttcttgttcc tcgctaccca tctgacgaaa tacagcctga ataaaactgt
     4081 gcaacgtctg agcatcaaag cgatgaccac tttccatggt taactcctgt ttatgttatg
     4141 tgtttgttgt ttttttatgc tgcacgccgg gcgcgggaag gtcaataaag atgcaggaat
     4201 ttcatattgc ggtggggtaa aagccggatg acatggctca tccggctgag aaaagaatta
     4261 gaatgcctga ttaacgcgcg ccaggccgtc gctgatagtt gccgcttcac ccgtagccac
     4321 caggcagcaa gccatctgga ttttcagcga ttcgggaatc ggttcgctgc cagccaggca
     4381 acgctcaatc cactgcgccg tggtttccgg atcttttgct tgtggcagta actcgctacc
     4441 agcagtgtcc tgtttttcat acagcacccg catcccttca cggtcaatga gattgatctg
     4501 cgggcagcgc tgcggattag catacacttc accttcagta ccatgcatta gtagcgcccg
     4561 cccgccgata tcgctaaaga acttcgcgac gcgtccaatg tattccggat gcgaaacgct
     4621 ggaaagacgc agtgcctcac cttcggcaaa tggcgtcgcc agtttcgcca gggtatgtgc
     4681 actgttacgc acgcccatcc gccagcgcat cgccagttgt ttttccagcg gcgggcaaaa
     4741 cgcgccgaca ggcataaaca ccggttgatg ttcgtcgagc ttcgcctgcg cctgcccgcc
     4801 gtgcaacgtt ggcgtaatac ccatcagttc gaaaatggtt tctgtcagca cgcgggttgg
     4861 atcttcgcta accccgtgaa ccaccacagg aaaaccgagt ttgtgcagga gaatcgccaa
     4921 caacggcgtc aggttggcct gtttacgcgc gccgttgtaa ctggggatga caatcggcat
     4981 cggcttgtct gcaggcggcg tcagcttgat ggtgtgattt tgcatggctt cgtaaaagcc
     5041 gagcatctct gcttcccctt cccctttgat acgcagcgca atcaatacgc cgcccaactc
     5101 aaggtcaggg acttcaccat tgagcatatg ggcatacaga ccgcgcgcag tatcccggtc
     5161 taaatcgcgc gcgtggtttt tcccgcgccc gatctcttta atgattttgc gatagtccat
     5221 ttacgactcc ttacctgact cacatcatta acgccgccga cggcgtgggg atttcgtttt
     5281 ttctttcttt ttaactatac cttcagggac ttccggttgc tctatcggaa ataccggtaa
     5341 tgcatccagt agccgcttgc cgtagttttt ggtgagcaag cgtttatcgt aaatcaccac
     5401 ttcgccccag caaccgtggc ttcgaatcag tcgcccaacc tgctgaatca ggttaaacga
     5461 ggcgctcggc aggctttgca cctcaaacgg atagcggttg aggcttttca gccattcccc
     5521 ttcggtgatc accacggggc tgtcgatggg cggaaaagcg attttgtgga tatgcacctg
     5581 gctgagcaga tcgcctttca gatcaagccc ttcggcaaat gactgtaagc ccaccagcac
     5641 gctgcgctca ccgttggcga cgcgtttgcg gtgcagttca actaaacggt aacgcggctg
     5701 atcgccctgc accagcaaca tcagacgtaa atccgtcaca tagtcgagaa agcgctgcat
     5761 cgcccgtccg ctggcaaaca gtaccaacat accgagatgt tttttgctct ccacctgctt
     5821 acggaaaaag gccgccattt cggcaatatg ctgctcttcg ttgtcgaggg aaggctcaac
     5881 gcgcatccgg ggaataacaa ttttgccctg ttcgcagtgg ttaaaggggg aatccagcgc
     5941 cacaaaacgg tcgcccgctt tctctttcag cccactcatc tcctgcaaac gcgaaaaact
     6001 gttcagcgaa cgcaaggttg cggaggtaac aataatgtgc ggaatactgc gccacagcag
     6061 cctttccagt tggtcgctaa cacgaatacc cacacagtga aaccagagat gcagctgccc
     6121 ttcgcgctct tcccgcgtcg cccatttggt caccggcgca ccggaagatt gcgccagaga
     6181 cgccagccgc cagagtttgc tttgcgcctc gaacatcccc aacgcgcggt tcatctgcaa
     6241 aatcaaccga tgcaggcgta caatgtcatg gctgccggtt ttctcactta aatcgttaag
     6301 aaataactcc gccaggccac gcagcatctc ggtgagtttt gccagccgct ggcagatctc
     6361 cagtacttca tcaggcagtt cgcccatcgc aaaacggtgc tctgcctcct gcccggcagg
     6421 catgtagagg ttgagaatgt tgtttaacga ggcgataagc tcatacaact cttcacaatg
     6481 ggcattcaaa cgttcaggga tcgccagcgg cgggatggtc ttcgggcgaa actgctccat
     6541 gcaggtagcg accagtttcg tgaacaagtc cagctgtagc cgataccacg gtgcggtgat
     6601 ttctgcactc atctccagcg catcccgcgc cacatccggc aggtgatgac cttcgtccag
     6661 caccagcagt aaatttttcg ggtcaggcaa tacggcttcg ctttccatcg ccgccatcac
     6721 cagcgcatgg tttgccacca ccacttccgc ttcctgaatc tcccgacgag cgacaaaaaa
     6781 cgggcattcg cgatagtaat aacagttgcg gttaaggcaa ctggccttgt cggtgctcaa
     6841 acgacgccag agatcgtcat ctatagcgat atcagtatga tcacgcagac catcccattt
     6901 ataagtgtcg agatcgccct tcagcttcgc acaacgtttt tgttcttcct gattgttcgg
     6961 cgtcagttcg tcgtcaagaa acgccagcaa atcctgttgc gtgggttcag tgctggcgag
     7021 cgccgtcaga ttacgcggac aaacgtagcg cccacgccca aaagcggcag tgaatttaag
     7081 atcgggaatg atctttttca gcagcggtaa atctttactg tagatctgat cctgcaatgc
     7141 cacgttggcg gtactcacca ccagcgtttt ttgctcttcg cgggcaatgg cgatgccagg
     7201 gatcaaatag gagagcgttt tcccaacgcc agtgggggct tcaatcgcca gatgccgccc
     7261 ttcttctccg gccagcgttt tggcgacgtc cgcaatcatc tgccgctgcg gcgcacgggg
     7321 aataaagtcg gggatctgtt cctgaagcgc cttataccag gcggcaattt gcgctttaag
     7381 cgcggcggtt aatgccatga gaaaacctga aatactgtat aaacagccaa tattgtggca
     7441 tttttcggtt ttcaggggta atggtttttc tgtttctgga aagcggctcg caataacgtt
     7501 ttctggctgt ggggttttct cgcaacagaa gaaacatcat tgcgtagtaa aagacaatgc
     7561 acgcaggctt attgcgctgg tggccattgc agggcggtat gtaaaacacg cagtaccgag
     7621 agcgtatcgc cctccacttg gtagatcagc acataatgcg gcctgacaac ggcttcatgt
     7681 gtaccagcga ctcgtccggc ccggtacatc tccgggcgtt cacaggcctg ttccgcaatg
     7741 gcttcaaatt gattatcaag ggctattgcc gctagcggat tatctttact gatgtattcc
     7801 atgatttgct ccctgtcaga cagggacatt ggcttccatt ccagacgcat taatccccct
     7861 tcagcaaggc ttttttacgg gcagcaaata gacttttggc ctcttcatga gagagcgccg
     7921 ggcgtggatc gtcaatggcg gcctgtacct gttcacgaaa ccaggcgtca tacgcggcag
     7981 cttcatgagc tcgttttaat gcctctgccc tgtctggtcg ggtcgttttc ttctgaccgt
     8041 ccgggtcgta atgagaggcg tcgacttcaa accgggcaat accaacatct ttcaggtagt
     8101 tgacaaccgt ttccagccgt ttaaacaagc gaatttgccg actgcgttgt gctgcaagag
     8161 gccgttcagt gtggccgtat ttcacggtca acgaccagcc tccctgggtg ccaataacgt
     8221 gtgctgcatc cactacgcca gattcaacca gtttatgtaa ggttgagtga tcgatcgttt
     8281 ctggtgccat aatgcgctaa cctctttgta tacggcatgc aatttataat taaagatagc
     8341 gcaattaata ataaattgca gcaccttaat cgtaaattgc ccaggataaa attgcggtag
     8401 aaagagattt gttgcgaata tgccgggcat cgcccggcat aaaagattac tgcgcagcgg
     8461 caggtttacg cggacggcga cggcgttgtt gttcgcctgc cggtttggca tcaccaagac
     8521 gacgagatgt tttttctgcg ggcttcgcgc tagctgattt cgcaccacct tctccacggc
     8581 gtggttgctg ttgaccgcga ccaccacctt gcccacgacc gccgccgcca cgttgctggc
     8641 gaccgttctg gatcggttcg gctttgattg acgggtccgg ctcataaccc ggaatcgcaa
     8701 tgcgcgggat ctcttttttc agcagttttt cgatatcacg cagcagtttg tgttcatcaa
     8761 cacacaccag cgacaacgct tcaccggtag cagccgcacg accggtacgc ccgatacggt
     8821 ggacataatc ttccggtacg tttggcagtt catagttgac cacgtgcggc agctcttcaa
     8881 tatccaggcc gcgcgcagcg atgtcagttg ccaccagtac acgaatatcg cccgatttaa
     8941 aatcagccag cgcacgggta cgcgcacctt gcgatttatt gccgtggatc gccgcactgc
     9001 ggatgccatc tttattaagc tgttcagcca gatggttagc gccatgtttg gtacgggtaa
     9061 acaccagcac ctgctgccag ttccctttcc caatcatatg cgacagcaat tcgcgtttgc
     9121 gtttcttatc gacaaagtga acgtgctggg tcacctgatc agacgcagta ttgcggcgtg
     9181 ccacttcgat ttccagcggg ttgtgcaaca gtttttccgc cagggcttta atatcgtcag
     9241 agaaggtcgc ggagaacaac aggttctggc gcttcgctgg cagttttgtt aacacgcgac
     9301 ggatatcgtg gataaagccc atgtcgagca tgcggtcagc ttcatcaagg acgaggattt
     9361 caacctgatc cagcttcact gcattttgat gttccaggtc cagcagacgt cccggcgttg
     9421 ccaccagcac atcaacgccg ccgcgcaact tcatcatttg tgggttaatg ctgacaccgc
     9481 caaacaccac cagcgaacga atgttcaggt atttgctgta atcacggacg ttttcgccaa
     9541 tctgcgccgc cagttcacgg gtcggggtaa gaatgagcgc acgtaccgga cgacgccctt
     9601 tggcgtgcgg ctggcgagtg atcaggtgtt gcaacagcgg cagcgtaaag cccgctgttt
     9661 tgccggtgcc ggtctgggcg ctggccatca ggtcgcggcc ttccaacacc gcagggatcg
     9721 cctgctgctg aataggggtg ggttcacggt aaccctgctc ggcaacggcg cgcaggatat
     9781 cagggcttaa acccaaagaa tcgaaagaca taactactcc gaaccgcccc gaccgttacc
     9841 ggtgtagttt tcagggagat actgaaaagg aaaatgacaa aaaccacaat gtcatgaggg
     9901 agcggagtgt agcagttttt gtgacgcagc gcataaatta tccctgcgac tggcgtagct
     9961 aaaattctta gccatactgg acaactcccc tatggagtca taatcttaat caatcatttg
    10021 attaagaatg gactgtgcga tgaataatcc tgccatgaca atcaagggtg aacaggcgaa
    10081 aaaacagctg attgctgcgg cactggcaca gtttggtgaa tatggaatga acgccaccac
    10141 ccgcgagata gccgcccagg ccgggcagaa tatcgctgcc atcacctact acttcggttc
    10201 gaaagaagat ttgtacctcg cctgcgccca atggattgcc gatttcattg gcgagcagtt
    10261 ccgtccgcac gctgaagaag ccgaacgttt gttcgcacaa ccacagccgg atcgggctgc
    10321 catccgcgaa ctgatccttc gcgcctgcag gaacatgatt aagctgctca cccaggatga
    10381 caccgtcaac ctcagcaaat ttatctcccg tgagcagctc tctcccacgg cagcctacca
    10441 cctggtgcac gaacaggtga ttagtccgct acacagccac ctgacacgcc tgattgccgc
    10501 ctggaccggc tgcgacgcta gcgatacccg catgatcctt catacccatg cgttgattgg
    10561 tgagattctg gcgtttcgtc ttggcaaaga aactattctg ttacgtaccg gctggaccgc
    10621 gttcgatgaa gaaaagaccg aactgatcaa ccagacggtg acctgtcata ttgacctgat
    10681 tttgcaagga ttatcgcaaa ggagtttgta gtgatgaaaa aacctgtcgt gatcggactg
    10741 gcggtagtag tacttgccgc cgtggttgcc ggaggctact ggtggtatca aagccgccag
    10801 gataacggcc tgacgctgta tggcaacgtg gatattcgta cggtgaatct tagtttccgt
    10861 gttggggggc gcgttgagtc gctggcggtg gacgaaggtg atgcgatcaa agcgggccag
    10921 gtgctgggcg aactggatca caagccgtat gagattgccc tgatgcaggc gaaagcgggc
    10981 gtttcggtgg ctcaggcgca gtatgacctg atgcttgccg ggtatcgaga tgaagaaatc
    11041 gctcaggccg ccgcagcggt gaaacaggcg caagctgcct atgactatgc gcagaacttc
    11101 tataaccgcc agcaaggact gtggaaaagc cgcaccattt cggcaaatga cctggaaaat
    11161 gcccgctcct cgcgcgacca ggcgcaggcc acgctgaaat cagcacagga taaattgcgt
    11221 cagtaccgtt ccggtaaccg tgaacaggac atcgcccagg cgaaagccag cctcgaacag
    11281 gcgcaggcgc aactggcgca ggcggagttg aatttacagg actcaacgtt gatagccccg
    11341 tctgatggca cgctgttaac gcgcgcggtg gagccaggca cggtcctcaa tgaaggtggc
    11401 acggtgttta ccgtttcact aacgcgtccg gtgtgggtgc gcgcttatgt tgatgaacgt
    11461 aatcttgacc aggcccagcc ggggcgcaaa gtgctgcttt ataccgatgg tcgcccggac
    11521 aagccgtatc acgggcagat tggtttcgtt tcgccgactg ctgaatttac cccgaaaacc
    11581 gtcgaaacgc cggatctgcg taccgacctc gtctatcgcc tgcgtattgt ggtgaccgac
    11641 gccgatgatg cgttacgcca gggaatgcca gtgacggtac aattcggtga cgaggcagga
    11701 catgaatgat gccgttatca cgctgaacgg cctggaaaaa cgctttccgg gcatggacaa
    11761 gcccgccgtc gcgccgctcg attgtaccat tcacgccggt tatgtgacgg ggttggtggg
    11821 gccggacggt gcaggtaaaa ccacgctgat gcggatgttg gcgggattac tgaaacccga
    11881 cagcggcagt gccacggtga ttggctttga tccgatcaaa aacgacggcg cgctgcacgc
    11941 cgtgctcggc tatatgccgc agaaatttgg tctgtatgaa gatctcacgg tgatggagaa
    12001 cctcaatctg tacgcggatt tgcgcagcgt caccggcgag gcacggaagc aaacttttgc
    12061 tcgcctgctg gagtttacgt ctcttgggcc gtttaccgga cgcctggcgg gcaagctctc
    12121 cggtgggatg aaacaaaaac tcggtctggc ctgtaccctg gtgggcgaac cgaaagtgtt
    12181 gctgctcgat gaacccggcg tcggcgttga ccctatctca cggcgcgaac tatggcagat
    12241 ggtgcatgag ctggcaggcg aagggatgtt aatcctctgg agtacctcgt atctcgacga
    12301 agccgagcag tgccgtgacg tattgctgat gaacgaaggc gagctgctgt atcagggaga
    12361 accgacggcc ctgactcaaa ccatggccgg acgcagcttt ctgatgacca gcccgcacga
    12421 gggcaaccgc aaactgttgc aacgggcatt gaaactgccg caggtcagcg acggcatgat
    12481 tcaggggaaa tcggtacgtc tgatcctcaa aaaagaggct acaccagacg atattcgcca
    12541 tgccgacggg atgccggaaa tcaacattaa cgaaactacg ccacgttttg aagatgcgtt
    12601 tattgatttg ctgggcggtg ccggaacctc ggaatcgccg ctgggcgcaa tattgcatac
    12661 ggtggaaggt acacctggcg agacggtgat cgaagcgaaa gaactgacca agaaatttgg
    12721 tgattttgcc gccaccgatc acgtcaactt tgccgttaaa cgcggggaga tttttggttt
    12781 gctggggcca aacggcgcgg gcaaatcgac cacctttaag atgatgtgcg gtttgctggt
    12841 gccgacttcc ggccaggcgc tggtgctggg gatggatctg aaagagagtt ccggtaaagc
    12901 gcgccagcat ctcggctata tggcgcaaaa attttcgctc tacggcaacc tgacggtcga
    12961 acagaattta cgctttttct ctggtgtgta tggcttacgc ggtcgggcgc agaacgaaaa
    13021 aatctcccgt atgagcgagg cgttcggcct gaaaagtatc gcctcccacg cgaccgatga
    13081 actgccatta ggttttaaac agcggctggc gctggcctgt tcgctgatgc atgaaccgga
    13141 cattctgttt ctcgacgaac cgacgtccgg cgttgatccc ctcacccgcc gtgaattttg
    13201 gctacatatc aacagtatgg tagagaaagg cgtcacggtg atggtcacca cccactttat
    13261 ggatgaagcg gaatattgcg accgcatcgg cctggtgtac cgcgggaaat taatcgccag
    13321 cggcacgccg gacgatttga aagcgcagtc ggccaacgat gagcaacccg atcccacgat
    13381 ggagcaagcc tttattcagt tgatccacga ctgggataag gagcatggca atgagtaatc
    13441 cgatcctgtc ctggcgtcgc gtgcgggcac tgtgcgtcaa agagacgcgg cagatcgttc
    13501 gcgatccgag tagctggctg attgcggtag tgatcccact gctactgctg tttatttttg
    13561 gatacggcat taacctcgac tccagcaagc tgcgggtcgg gatattgctg gaacagcgta
    13621 gcgaagcggc gctggatttc acccacacca tgaccggttc gccctacatc gacgccacca
    13681 tcagcgataa ccgtcaggaa ctgatcgcca aaatgcaggc ggggaaaatt cgcggtctgg
    13741 tggttattcc cgtggatttc gccgaacaga tggagcgcgc cagcgccacc gcaccgattc
    13801 aggtgatcac cgacggcagt gagccgaata ccgctaactt tgtgcagggg tatgtcgaag
    13861 ggatctggca gatctggcaa atgcagcgag cggaggataa cgggcagact tttgaaccgc
    13921 ttattgatgt acaaacccgc tactggttta acccggcggc gattagccag cactttatta
    13981 tccccggtgc ggtgaccatt atcatgacgg tcatcggcgc gattctcacc tcgctggtgg
    14041 tggcgcgaga atgggaacgc ggcaccatgg aggctctgct ctctacggag attacccgca
    14101 cggaactgct actgtgtaag ctgatccctt attattttct cgggatgctg gcgatgctgc
    14161 tgtgtatgct ggtgtcagtg tttattctcg gcgtgccgta tcgtgggtca ctgctgattc
    14221 tgttttttat ctccagcctg tttttactca gtaccctggg gatggggctg ctgatttcca
    14281 cgattacccg caaccagttc aatgccgctc aggtcgctct gaacgcggct tttctgccgt
    14341 cgattatgct ttccggcttt atatttcaga tagacagtat gcccgcggtg atccgcgcgg
    14401 tgacgtacat tattcccgca cgttatttcg tcagcaccct gcaaagcctg ttcctcgccg
    14461 ggaatattcc ggtggtactg gtggtgaacg tgctgttttt gatcgcttcg gcggtgatgt
    14521 ttatcggcct gacgtggctg aaaaccaaac gtcggctgga ttagggagaa gagcatgttt
    14581 catcgcttat ggacgttaat ccgcaaagag ttgcagtcgc tgctgcgcga accgcaaacc
    14641 cgcgcaattc tgattttacc cgtgctgatt caggtgatcc tgttcccgtt cgccgccacg
    14701 ctggaagtga ccaacgccac catcgccatc tacgatgaag ataacggcga gcattcggtg
    14761 gaacttactc aacgttttgc ccgcgccagc gcctttaccc atgtgctgct gctgaaaagc
    14821 ccgcaggaga tccgcccgac catcgacaca caaaaggcgt tactcctggt gcgtttcccg
    14881 gctgacttct cgcgcaaact ggataccttc cagaccgcgc cgttacagtt gatcctagac
    14941 gggcgtaact ccaacagtgc gcaaattgcc gccaactacc tgcaacagat cgtcaaaaat
    15001 tatcagcagg agctgctgga aggaaaaccg aaacccaaca acagcgagct ggtggtacgc
    15061 aactggtata acccgaatct cgactacaaa tggtttgtgg tgccgtcgct gatcgccatg
    15121 atcaccacta tcggcgtaat gatcgtcacg tcactttccg tcgcccgcga acgtgaacaa
    15181 ggtacgctcg atcagttact ggtttcgccg ctcaccacct ggcagatatt tatcggcaaa
    15241 gccgtacctg cgttaattgt cgccacgttt caggccacca ttgtgctggc gattggtatc
    15301 tgggcgtatc aaatcccctt cgccggatcg ctggcgctgt tctactttac gatggtgatt
    15361 tacggtttat cgctggtggg attcggtctg ttgatttcat cacactgttc aacacaacaa
    15421 caggcgttta tcggcgtgtt tgtctttatg atgcccgcca ttcttctttc cggttacgtt
    15481 tcgccggtgg aaaacatgcc agtatggctg caaaacctga cgtggattaa ccctattcgc
    15541 cactttacgg acattaccaa gcagatttat ttgaaggatg cgagtctgga tattgtgtgg
    15601 aatagtttgt ggccgctact ggtgataacg gccacgacag ggtcagcggc gtacgcgatg
    15661 tttagacgca aggtgatgta attttttatc tttcgccagc aaagacacta ccgccgggcc
    15721 agcaaggatt gccagccctg caattgccag caaaaagttg ttttccagta cacgggacaa
    15781 cagccacagc acaatcagcg ctgcgccaaa ataccaggtc gtggtcagct cttccagcac
    15841 gtctgccacc tcacgccagc gttgctcggg gtggcgctga aacaccagca tcatcactac
    15901 cgtgacacaa tacagtgcgc ataatcccac gccgacgtga accagagtct tactcatcca
    15961 gcccaccacc agcagcgcca ctaccagtaa atgcaacata atctgccagc aacttaggcc
    16021 agttgcgaca cgaacacgtt gttgccactt catggcttct ctcctgaaga atttatctct
    16081 acttattcag agtaccgaac tttacggaaa attccgtaag accgcacctt tttgtgacgc
    16141 cgactacact atttcaatga gataagaggc aaagacagga aaattatgcc cgatcaaaca
    16201 caacaatttt cgttcaaggt gctcaccatc aatattcaca aaggctttac cgcgtttaac
    16261 cgacgcttca ttttgccgga acttcgcgac gccgtgcgta ccgtcagtgc cgatattgtt
    16321 tgcctgcagg aagtgatggg cgcgcacgaa gttcatccgc tgcatgtgga aaactggccc
    16381 gatacctcgc actacgagtt tctcgccgac accatgtgga gcgatttcgc ctacggtcgc
    16441 aatgccgtat acccggaagg acatcacggt aatgcggttc tttcacgtta tcccattgaa
    16501 cattatgaga atcgtgatgt ttcggtcgat ggtgcggaaa agcgcggcgt gctctactgc
    16561 cgcattgtgc cgccgatgac cgggaaagcg attcatgtga tgtgcgtaca tctgggcctg
    16621 cgtgaggcgc accgtcaggc gcagcttgcg atgctcgccg aatgggtgaa tgagctgccg
    16681 gacggcgaac cggtattggt ggcgggtgat ttcaacgact ggcggcaaaa agctaatcat
    16741 ccgttaaaag tgcaggccgg gctggatgag atatttaccc gcgcccacgg acgcccggcg
    16801 cgcacgtttc cggtgcaatt tcctctacta cgactggaca ggatctacgt caaaaatgcc
    16861 agcgccagcg cgccaaccgc gttgccgctg cggacatggc gacacctttc tgatcatgcc
    16921 cctttaagtg cggagattca tttatgaaat gtagctggcg cgaaggcaat aagatccagt
    16981 tgctggaaaa cggcgagcaa tattatcccg cggtgtttaa ggcgattggc gaggcgcaag
    17041 aacgcatcat tcttgaaacg tttatctggt ttgaggatga cgtcggcaaa cagctgcatg
    17101 cggcgctact ggcagcagcg caacgcgggg tgaaagcgga agtcttgctg gatggctacg
    17161 gttcgccgga tctcagcgat gagtttgtca atgaactgac ggcagctggc gtggtatttc
    17221 gctactacga tccccgcccg cgcctttttg gtatgcgcac caatgtgttt cgccggatgc
    17281 atcgcaaaat tgtggtgatc gacgcgctta tcgcctttat tggcgggctg aattactccg
    17341 ccgagcatat gtccagctac ggtccagagg ccaaacagga ttacgcggta cgccttgaag
    17401 ggccgattgt tgaagatatc ctccagtttg aacaggaaaa tctgccagga cagagcgcgg
    17461 cccgacgctg gtggcgacgt catcacaaag cggaagaaaa tcgccagccg ggagaagcgc
    17521 aggtattgct ggtctggcgc gataacgaag aacatcgcga tgatatcgaa cgtcactatc
    17581 tgaaaatgct cactcaggcg cggcgagaag tgattatcgc caacgcctac ttcttccccg
    17641 gctatcgatt tttacacgcc ttgcgtaaag cggcacggcg cggggtgcgg atcaaactga
    17701 tcattcaggg cgaaccggat atgccgattg tcagagtcgg tgcgcgtttg ctgtataact
    17761 atctggttaa aggcggcgtt caggtgtttg agtaccgccg ccgtccgcta catggcaaag
    17821 tggcattgat ggacgatcac tgggcgacgg taggatccag taatctcgat ccgctcagtt
    17881 tgtcactgaa tctcgaagca aatgtcatca tccacgatcg tcattttaac cagacgctgc
    17941 gtgataatct gaacggcatt atcgccgcag attgtcagca ggtggatgag accatgctgc
    18001 cgaaacgcac ctggtggaat ctgaccaaaa gcgtgctggc cttccacttt ttacgccact
    18061 tcccggcgct ggtcggctgg cttccggcac acacgccacg tctggcgcag gttgatcccc
    18121 ccgcacaacc gacaatggaa acgcaggatc gggtagaaac tgaaaacacg ggggtaaaac
    18181 cctgatgagt aaatcacacc cgcgctggcg cttagcaaag aaactcctca cctggctgtt
    18241 ttttatcgcg gtgattgtgt tactggtggt ctacgccaaa aaagtggact gggaagaggt
    18301 ctggaaggtc atccgcgact acaatcgcgt tgcgctgctt agtgcggtcg ggctggtggt
    18361 cgtcagttat ctgatttacg gttgctacga cctgctcgcc cgtttctact gcggtcacaa
    18421 actggcgaag cgccaggtga tgctggtgtc gtttatctgc tacgccttca acctgacact
    18481 cagtacctgg gtcggcggca ttggtatgcg ctatcgtttg tactcccggc tagggttacc
    18541 gggcagcact attacgcgga ttttctcgct cagtattacc accaactggc tgggctatat
    18601 tttgctggca gggattatct ttaccgcagg cgtggtggag ctgccggacc actggtatgt
    18661 cgatcaaacc acgctgcgca ttctcggcat tggcttactg atgattatcg cggtttattt
    18721 gtggttttgc gctttcgcga agcaccgcca tatgaccatc aaaggacaaa aactggtgct
    18781 gccttcatgg aaattcgccc tcgcccaaat gctgatttcc agtgttaact ggatggtaat
    18841 gggggcgatt atctggctgt tacttggtca aagcgtgaac tatttctttg tactgggcgt
    18901 gttactggtt agtagtattg ctggcgtcat cgtgcatatt ccagcgggga ttggtgtgct
    18961 ggaagcggtg tttatcgcgc tactggctgg ggagcataca tccaagggca caattatcgc
    19021 cgccctactc gcttaccgtg tgctgtatta ctttatcccg ctgctgctgg cgctggtttg
    19081 ctatctggta ctggaaagcc aggcgaagaa gctgcgggcg aaaaatgaag cggcgatgtg
    19141 aagtatagtt aagttcttta gtatgtgcat ttacggctaa tgaaaaaacc gcgtatgcct
    19201 ttgccagaca agcgttatag ctatagcggc aagaatccca aagaagccga taaaatagta
    19261 atagagtgtt aatgcaccta taaaagcaca acgaaccgga ctccataatt cttcactatg
    19321 gcattttcct gccagttcaa ggagcttatt tcgtccatgc gagataatgc ctgaccataa
    19381 gacaatcatt aagcaactta ttacccaaat gggtcgttca ctcattaatg tggcattcac
    19441 gatgattacc agggccaacc ctgtcaacgt catcataatg gtaaaacggt gagagccagg
    19501 atcaacattg aataataaac cagccagcat actgataaaa aacatcgcgc ccgctgtgct
    19561 gaatacgacg ataatggcag atacaggcgc taaactcgca acgacaatcg ccaccaaaga
    19621 gattaaaaac aaatatatta cgcccgcgat ggcggtattt atcttgttga tatctttact
    19681 taagccatac atagaaagta ataataatcc tatagcaata tacatttccg gtgaatcata
    19741 ttttgttgcc gtaatttcga gatacttagc gccccaggcc agccctgcgg ttgccaccaa
    19801 acccaaagcg gtaatgagcc atatgccgtt taatatcctg gcttcgtgct gaaaatctaa
    19861 tttattgcta ttttgcgagt atgattccat tcttttagtt gtcctcagaa aaaacattaa
    19921 tattcaccat caataaattt aaaaccacgc atacaaaata cttttgcatt tatggataca
    19981 gaaaacactt ccgctcggcc ataaggcata ttttatacct ggtacttgtt aagtgtcgtt
    20041 tttagtaaga aaaaaaatcc cgacacatgg ccgggatatt gatggtgaaa aaagaattaa
    20101 cggcggttgc cgaagatccg cagcaacatc aggaacaggt tgatgaagtc cagatacaag
    20161 gtcaacgcgc caagaatgga atatttgcgc aggttcgacg tgtcgcgggt atcaatctgc
    20221 tcacccatat ttttcagttt ctgggtgtca tacgccgtca atccgacaaa gacaatcacg
    20281 ccgatgtatg tgactgccca catcaacgct tcgcttttca gccagaagtt gaccagcgat
    20341 gccagcacaa tgccgatcaa cgccataaac agcatattgc cgaaaccact tagatcgcgc
    20401 ttcgtggtgt aaccgtacag gctcattgcg ccgaacatcc cggcagtaac gacgaaagta
    20461 ctggcgatag aagcagcggt atagacaatg aaaatactgg aaagcgtcag gccagtcagc
    20521 gcggaataaa gcataaagag catcgtcgtt acacctgcgc tcagcttttg aatcatcgct
    20581 gataacacaa taaccagagc taattgtgct attaccagac caattaaaaa gatgcggttg
    20641 gtgaataata gctccataac ggccgcggaa ttagccgcat accaggcaac aaacgcagta
    20701 agcagcaagc ctactgtcat ccagccatag acctgtgcca tataggtttg caggccagtt
    20761 cgggtttgta cgattgaatc agaacgtggg aatctgtcca tgacgatctc ctgaagatat
    20821 aaggaatatc ttaaggatac tgcaaaatga tgaggctgtg catcgacgca gcgtaaacgc
    20881 atatactgag cggtgaaatt gccggacgca gcggtgcctt atccggctaa caaaaaacta
    20941 ccagcgtttt gccgcctgct gatcgctctc ccgagcttcg acccagcggt cgccttccgg
    21001 cgtggcttcg cgcttccaga acggtgcgcg ggttttgaga taatccataa taaactgccc
    21061 ggcttcaaac gcactgctgc gatgcgcact ggtgacaccg acaaaaacga tttcatcgcc
    21121 cggccataat tccccgatgc ggtgaatcac cgtgacgcgc cccagcggcc agcggttacg
    21181 cgcttcatcg acaatttctg ccagtgcttt ttcagtcatc cccggatagt gttcgagggt
    21241 taatgctttg acgctgtcgc caaggttatg gttgcgcacc ttaccggtaa aggtgactac
    21301 cgcaccgtct tcatcacgct ccgccagcca cggatactct tctcctacgc tgaacggctg
    21361 cgggccgata acaattttgg tttctgccat cttaacctcc ggttaccggc gggaagaaag
    21421 ccacttcgtc gccgtcagtc agcggatggt caaaactcac cagcgtctgg ttgacagcag
    21481 ccagcaattt gccatcttcc agcgccagcg cccagcgatc gctctgcgca gccagatgtt
    21541 ggcgtaacgc ttcaacggtt gggaaatccg cagctacttc ggttgcatct gttcccacca
    21601 actcgcgcac ctgggcgaaa aaaagaactt taatcatccg cttccacctt aaagtcaccc
    21661 gacttgccgc cgctcttcgc cagcaaacgt accggaccaa tcaccatatc tttttgcacc
    21721 gctttgcaca tgtcataaat ggtcagcgcc gccacggagg ccgcggttaa tgcttccatt
    21781 tcgacaccgg ttttcccggt caggcggcat aaggtttcta tacgtacccg attgtgctcc
    21841 ggctcagcct gtaaattgac ttcaactttg ctgagcatca gcgggtgaca cagcgggatc
    21901 agatcccagg tgcgttttgc cgcctgaata ccggcaatac gcgcagtggc aaatacgtcg
    21961 cctttgtggt ggcgaccatc aataatcatc gccagcgtct cgctgcgcat ggtgacaaag
    22021 gcttcggcgc gcgcttcacg cacggtttcc gctttggcgg agacatccac catgtgcgct
    22081 tcgccagcgg cgttgatatg ggtcagttgc gacatactta tttcttcaaa tgtggatgga
    22141 aattacacgg acgcgtacgg gcatccagct gcggcgcaat gatattttcc catgcggtac
    22201 ggcacgcttt ggtcgaaccc ggcatggcga aaatcagcgt tttgttagcg acgcccgcta
    22261 ccgcacgaga ttgcaacgtg gaagtgccaa tctcttcgaa cgacaacatc cggaacactt
    22321 caccaaaacc ttcaacttca cggtcgaaca acggcagcag cgcttcggga gcctgatctc
    22381 cttccgtcag gccagtacca cccgtaatca acaccacttg tacatcgtcg ctggcgatcc
    22441 acgcagatac ctgagcgcga atagcgtagc ggttttcttt cacaatggct ttatcgacaa
    22501 cgtgatggcc cgcttcttgc gccgagtcgc gcagatagtg accggaggta tcgtcttctt
    22561 caccgcgacg attagaaacc gtaagaatag caatacgggt cgggataaat tcagtgctta
    22621 cctgactcat ctgatctctc cttttgacgt tttagccacc aatgtacgat aagttttgcg
    22681 taataccggt gttgttttga tgcaggaaat gggtctgttt tttctcccgc agcgccgctg
    22741 aaatacgcgc ttccagcgcc tgttgctggg tatcgtcttc cagcagatcg cgcaggttaa
    22801 cgccgccttc accaaacagg cagagatgga gtttaccaat ggaggaaacg cgcaggcggt
    22861 tgcaagtggc gcagaagtct ttttcatacg gcatgataag gccaatctct ccggcgtaat
    22921 ccggatgaca aaagacttgc gcgggaccgt cgctgcgttg acgtaattgg tggatccagc
    22981 cgcgacgcag tagctcgtca cgcagaacct gaccagagat gtgatgctta cggaagagct
    23041 cgctgccctc gcccgtttcc atcaattcga tgaaacgcag ctggataggg cgatgctgga
    23101 tccagttcag aaaggtgtcg agctgatgat gattaacatc acgcatcagc acggtattga
    23161 ctttgacctt ctcaaaaccg gcctcaaatg cagcatcgat tcctgccatg acctggttga
    23221 atttatcctg cccggtaatg gcatgaaact ggcgggcgtc cagactatca acgctgacgt
    23281 tgatgccagt aagtccagca tcgcgccagt tcgccacatc gcgttccaga cggtaaccat
    23341 tggtggtgac agcaatctgg cggatagcgt cgttttctcg cacagcggcg atgatatcgg
    23401 taaagtcgcg gcgtaaagac ggctcaccgc ccgtcagacg gactttttcg gtgcccagac
    23461 tggcgaaggc gcgcgtaacc cggcgaattt catcgacggt aagaaagcct ttattggtga
    23521 cgccgctcgg tttgtagcca tccggcaggc agtaggtgca acgaaagtta cacacatcgg
    23581 taatcgacag gcgcaagtag taaaacttac gcgcaaatgc atcagtcagt tgtgaagcca
    23641 tgtacacctt tccagatacg ggaggcgaag tcatttcttc cttcgccctg gtggcaatct
    23701 ttacgagcat tgtcacggcc aaagcaccgt atcagttgac ccaggtgcag aggctagagt
    23761 gtttagtggt taagccgata ctagcgtgta aatgagattt ttaccatcca cactttcgct
    23821 atgtaatcat gtatatagcg tcatgatcgt gctatttcgc tacagaataa gggaaaatgg
    23881 cggttttact ttgatataca tcatcaggaa ggcatagcgt acatcgtcct ttagaggtag
    23941 aagaaaatag tttgtatcct caatattggc aggttaattg ctgtttcccc gcaatttgcg
    24001 ctactgtagc gcggataatc tgactccagg aaactatatg cgcaatcgta cgctggctga
    24061 ccttgatcgt gtcgttgctc tcggcggagg gcatggactg ggacgcgttc tctcatcact
    24121 ttcgtctttg ggttctcgtt taacgggtat cgtcaccacc acggataatg gtggctcgac
    24181 ggggcgtatt cgccgctcag aaggcggtat tgcctggggc gatatgcgta actgcctcaa
    24241 ccagctgata actgaaccca gcgtcgcttc cgcaatgttt gaatatcgtt tcggcggtaa
    24301 tggcgaactt tccgggcaca accttggaaa cctgatgctg aaggcactgg atcaccttag
    24361 cgtgcgacct cttgaagcga tcaatctgat tcgcaatctg ctgaaagtgg acgcacattt
    24421 gattccgatg tcggagcatc ctgttgatct gatggcgatt gacgatcagg ggcatgaagt
    24481 ttacggcgag gtcaatatcg accagttaac tgcgccgatt caagagttat tgttaacgcc
    24541 gaatgtaccc gcaacgcgtg aggcggttca cgctatcagt gaagcggatc tcatcattat
    24601 tgggcctggc agtttttaca ccagcctgat gccaattctg ctgctgaatg aaatcgcaca
    24661 agcattacgc cgcacgccag cgccgatggt ctatatcggc aatctggggc gtgagttgag
    24721 tttacctgcg gctaatttga agctggaaag caagctggca attatggagc agtatgtcgg
    24781 taaaaaagtg attgatgcgg tagttgttgg gccgaaagct gatgtatccg cagttaacaa
    24841 cagaattgtg attcaggaag tactggaggc cagcgatatc ccctatcgtc atgaccgcca
    24901 gttgttacat aacgcgctgg aaaaggcatt acaggcttta ggttaacacg aagccggaag
    24961 aatattccgg cttcgtcatc ttgtcacgac gcggcaataa acagctcacg cagctgatgc
    25021 aactggtcac gaatttgcgc cgcttcttcg aactccagat tctgcgcgtg ttgcatcatc
    25081 aacccttcca gttcatggat cttctgctgc aacgctttag gcgacatatc catcggcaca
    25141 ttatctggct caacaatcgg gcgcgatttt cctctgccct tcgctttggt tttggcaatg
    25201 ttctgcccca gcgccaggat atcgaccact ttcttgttca agccttgcgg cgtaatgccg
    25261 tgttcctcgt tgtacttctg ctgtttctcg cggcgacgtt cggtttcgcc aatcgctttc
    25321 gccattgatg gggtgatctt atcgccgtag agaatcgctt taccgttaac gttacgtgcc
    25381 gcacgaccaa tggtctggat caacgaacgt tcggaacgca ggaagccttc tttgtcagcg
    25441 tcgaggatcg ccaccagcga cacttccggc atatccagac cttcgcgcag taagttgatc
    25501 cctaccaata cgtcgaactc acccagacgc aagtcgcgga taatctccat acgttcgacg
    25561 gtgtcgatat ctgagtgaag ataacgcacg cgctcaccgt gttcttcgag atattcagtg
    25621 agatcttccg ccatccgctt ggtcagagtt gtaaccagta cgcgttcgtt aattgccgct
    25681 cgctgacgaa tctccgatag aagatcatcg acctgtgtcg ctaccggacg cacttcaata
    25741 atcgggtcaa gtaagcctgt tggacgcacc acctgatcca ccacatcgcc gccggatttt
    25801 tccagctcgt aattacccgg cgtcgccgaa acatagatgg tttgcggcgc taatgcttcg
    25861 aactcttcaa atttcagcgg acggttatcc agcgctgatg gcaggcggaa gccgtactcc
    25921 accagcgtct ctttacgcgc ccggtcaccg cgatacatgc cgccaatttg cggaatggta
    25981 acgtgagatt catcgacgac cagcagccca tcggcaggca ggtaatcaaa cagcgtcggc
    26041 ggtggctcac ccggtccacg accggagagg aagcgcgagt agttttcaat ccccgaacag
    26101 tagcccagct cgttcatcat ctccagatca aactgggtac gctgggtcag ccgctgctct
    26161 tccagcagtt tgttgttttc caacagcacc ttgcgtctgg cagccagttc ttctttaatt
    26221 tcttccatcg cctgcacaat gcgctcgcgc ggtgtgacgt agtgcgtttt cgggtagatg
    26281 gtaaaacgtg gaatagtgga aacaatctgc ccggtgagcg gatcaaataa cgacaatcgt
    26341 tccacttcct cgtcaaacag ttccacgcga agtgcaatgt catccgattc tgccgggaag
    26401 atgtcaatca cctccccacg aacgcggaaa gtaccgcgct ggaaggcttg atcattacga
    26461 gcgtattgca gctccgccag tcgacgcagg atcgcgcgct gatcgataat cataccgacc
    26521 gtaagatgga gcatcatctt gagatataaa tcaggatcgc ccagaccata aatcgcggaa
    26581 accgatgcca ccacaaccac atcacgccgc tccagcatcg ctttggtggc ggacaaacgc
    26641 atctgctcga tatgttcgtt taccgaggca tctttctcaa tgaaggtgtc ggaactcggt
    26701 acataggctt ccggctgata atagtcgtag taggagacga aatactccac cgcgttttcc
    26761 gggaagaatt ctttcatttc gccatacaac tgggccgcca gcgttttgtt gggcgcaagt
    26821 accatggtag ggcgctgaag gtcagcaatg acattggcaa tggtgaaggt tttcccggag
    26881 ccagtcacgc caagtaacgt ctggtgcgcc aggccatctt ccagcccctc ttcgagacgt
    26941 cgaatcgcct ctggctgatc gccagaaggt ttaaaagcgg aattcagttt gaacggttta
    27001 ctcatgagtc gctacctgaa ggagttgggc gggcaggtat gtaattttac tcgtcgtact
    27061 taattatgcc aacaaattat actggataaa aaaacagttt ctcaccataa tatttctgtt
    27121 acagcgtaaa ctccgctgtc aatcatgagc aaaatttact ctgtggcgag ataaaactcc
    27181 ggccttaccg ggttatcccc aaagcaacgg cttttttaac atttgtcaag atgattgatg
    27241 acagttttat ggcaagagat gcctgttcag tgactgccat tgcaattatc taacctgtta
    27301 aaaaataaaa aatatttctt tgagccgtct ttaacgccaa ttcgcgtgca agccgcgtat
    27361 tctctcgctt gcctcgtgtt ttctaactct aatacacatg gttatccaca ggaatagtgg
    27421 ataactgtct gcagccccta tcccccgccg cctgggcaat tcccacattc accgcatgat
    27481 gcggaggtaa aatttttttc gtgatttttt tagaatttaa ttggttaaat tgcagtcaat
    27541 cgaagacgcg atctcgctcg caatttaacc aagtacagga tgactacatt aaggcaagat
    27601 tgatgtactt tccggttgcc gcattttctg gattttctgc aagccaggga atttcgccca
    27661 gcagcggagc gggaatcatg cgggtgagcg tggtcatata ttcagcgtga cgttttcccg
    27721 gaggcgtgac atcattcgcc acccaacccg ccagagtcag tccggcgtgt tgtattgcct
    27781 gtgccgtcaa catcgcgtga ttaatacagc cgagtttcac accaactacc agtatcaccg
    27841 gcagttgttc ctgtgttacc caatccgcaa aagtgaaagt gtcagaaagc ggcgtaaacc
    27901 agccgccagc accttccact aacacccagt cagcctgttg ttcaagcgcg cgtaatccgg
    27961 cgctcattac caatgattct atcggcctgc cctcttgtgc gctgatgatg tgcggcgaag
    28021 tgggttctgc gaaggtgtaa ggatttaccg ttgcgtaatc cagctgcagg ctgctgttgc
    28081 gctgtaacgc cagcgcgtcg ctattacgta agccttccgg gatcttttcg ctgccagagg
    28141 cgaccggttt ataacctgcc gtccggtagc ctactgcctt tgcggcttgt aaaagtgcac
    28201 aactggcgac ggttttcccc acttcggtat ccgttccggt gacaaaataa cgtttactca
    28261 cgagcaatca ctcccaaaaa aagatgatac gtcagaggat atcgcccctg tttttgtggc
    28321 caggccagtt gcaatcgctg caactgcgaa cgcgttaata tccgcgggtc gcgcccttca
    28381 tgaagatgcg tggcaccgat gcctttcagc gaacgcatgg cactgagcgc atcatcaaac
    28441 cacagcgtga tgggctgaat atgatgttga taatgcaggc cgttcagcga ctgttcgatt
    28501 tcatctggcg gtaaaaagcg attagcatgc gggcgctcgt ccaccgcctg ccacgcctga
    28561 tgtaattcag ggagcgatcc ctgcaccagc gtggtaaacg cgaccacgcc tccggggcgc
    28621 accaccctgt atagctcgcg gagtgccgtg gataaattac cgcaccactg taccgcgaga
    28681 ttgctccatg caagatcgaa ggttgcagtc gctaacggca gggattcgat atctcccgcc
    28741 agataatggt ctgcggcatc cttctggcgt gcctgaacaa gcattggcgg agagagatct
    28801 aacgccgtca cctgcgcgtg acgttcccgc cagtggcggc tcatccagcc aggtccacaa
    28861 cccgcatcca gtacgtgggt gtatttacgc tgtgggagca ttgccagtaa gacgtcagca
    28921 ctctggcgct gtagatccgc atgttgctca tagtgcgcgg ctgcccgacc aaatgccgct
    28981 gcaatggctt gtttattaac cgttgtcatg cagcacctcc agcagacggt cgatatcctg
    29041 catttcatgc gcggcggtta gtgttaagcg cagtcgcgca gtaccagcag gtacggttgg
    29101 cgggcgaatc gccgtgaccc agcaaccttg ctggcgcagt ttttctgcca gttgtaacgc
    29161 acggctgtta tcaccgacga tcaatggctg gatggcgctc catgaatcag caagcgtaaa
    29221 cggcaaatcc tgcactccgg cacgaaaacg cgtaatgagt gccgccagtt tttcgcgccg
    29281 tgcatcaccc tcatcactgc gaatgaccgc cagcgacgca cgtaatgcct gcgcctgagc
    29341 gggcggcata ctggtgctgt agataagatg gcgggcgaat tgcagcagat aatccgccac
    29401 cgtattggag caaagcaccg ctgccccgct gacgccaaat cctttgccaa aagtcaccac
    29461 cagcaattct ggttttacct tttgcagcca gcagctgcca cgcccctgct ccccgataac
    29521 gcccgtgccg tgggcgtcat cgaccatcaa ccagccatcg tgctgttgcg ttacctgctg
    29581 gatttcctcc agtggcgcac tatcgccgtc catgctgaac acgccttctg tcactaccag
    29641 ttgctgcccc ggacaggggg aagcaagcag tcgcgccaga tgagtgacat cgttatgagc
    29701 aaaacggcga agcggcgacg ggcttaaact tgcagcctcc agcaatgagg catggctaag
    29761 ccggtcggca acaatacggt cctctttcgc catcatcgcg gtaataactg cctggttagc
    29821 ggcaaaacca gagataaaca gcagtgcccg cgaatagccc agccactcgg ccagttcttc
    29881 ttccagcgcc tgatgcgcca cgctataacc gctgacgtga ccggagccgc cgctaccgac
    29941 gccaaattgc tccgcactca gcttccaggc acggataatt tgcggatgat ggcttaaacc
    30001 taaataatcg ttactggaaa agttcagata atggcgatcg tccgccacca gccagcgtcc
    30061 ggctccttgc gccactggat aacggcgacg cagggcatcg gcagcacgcc gcgcatcgag
    30121 cgccgcgttg attttctcct gccagctcat agtgctgccg cgttgtaata ttcgtcagtg
    30181 tccggggtca tcagcgcctg ctccagacgc tgctgttgtt cgttatcgcc cgccagcacg
    30241 gcagtttgct gcggatttag ccccagtttg cggaacagtt gcaggtcttt atcttcttcc
    30301 ggattcggcg tggtcagcag tttgcaaccg tagaaaatcg agtttgcgcc agccataaag
    30361 cacatcgcct gagtctgttc gttcatctgc tcgcgtccgg cagaaaggcg tacgtaagag
    30421 gtcggcatca tgatccgcgc aactgcaatg gtgcgaataa aatcaaaggc atcgacatca
    30481 tcgttatcag caagcggcgt gcctttcacc ttcaccagca tgttgattgg tacgctttcc
    30541 ggcggcgttg gcaggtttgc cagttgcagc aataatccgg cgcgatcttt tactgtttcg
    30601 cctaagccca caatgccgcc cgagcagact ttgatcccgg cgtcgcgcac tttttccagc
    30661 gtatcgaggc gttcctgata ggtacgcgtg gtgatgatat tgccgtaaaa ctccggcgag
    30721 gtgtcgaggt tgtggttgta gtaatccagc ccggcgttcg caaggcgctg cgcctgagat
    30781 tcactcaacg tacccagcgt catacacgcc tccagcccca tcgcttttac tccctgcacc
    30841 atttgttcca ggtagggcat atcgcgttcg tggggattct tccacgccgc gcccatgcag
    30901 aagcgcgtcg atcctgccgc tttcgctttg cgcgccgact ccagcacctg ttcaacttcc
    30961 atcaaccgct cggcttccag cccggttttg tagcgcgagc tttgcgggca gtatttgcaa
    31021 tcttccggac aagctccggt cttaatcgac agcaaagtgc tgacctgcac ctgacgtgga
    31081 tcgaaatgct gacgatgcac ctgctgcgct tcaaacagca gatccagcaa cggtttttca
    31141 aataattctg tgacttgcga caatgtccag cgtgggcggt gagccatagg gcttctccaa
    31201 aacgcgtttt ttgttgttaa ttcggtgtag actcgtaaac ctaaatcttt tcaatttggt
    31261 ttacaagtcg attatgacaa cggacgatct tgcctttgac caacgccata tctggcaccc
    31321 atacacatcc atgacctccc cgctaccggt ttatccggtg gcgagcgccg agggttgcga
    31381 gctaattttg tctgacggca gacgccttgt tgacggtatg tcgtcctggt gggcggcgat
    31441 ccacggttac aatcacccgc agcttaatgc ggcgatgaaa tcgcaaattg atgctatgtc
    31501 gcatgtgatg tttggcggta tcacccatgc gcccgccatt gagctgtgcc gcaaattggt
    31561 ggcaatgacg ccgcaaccgc tggagtgcgt ttttctcgcg gactccggct ccgtagcggt
    31621 agaagtggcg atgaaaatgg cgttgcagta ctggcaggcc aaaggcgaag cgcgccagcg
    31681 ttttctgacc ttccgcaatg gttatcatgg cgataccttt ggcgcgatgt cggtgtgcga
    31741 tccggacaac tcaatgcaca gcctgtggaa aggctatctg ccagaaaact tgtttgctcc
    31801 cgccccgcaa agccgcctgg atggtgaatg ggatgaacgc gatatggtgg gctttgcccg
    31861 cctgatggcg gcgcatcgtc atgaaatcgc cgcagtgatc attgaaccga ttgtccaggg
    31921 cgcaggcggg atgcgcatct accatccgga atggttaaaa cgaatccgca aaatgtgcga
    31981 tcgcgaaggt atcttgctga ttgccgacga gatcgccacc ggatttggtc gtaccggcaa
    32041 actgtttgcc tgtgaacatg cagaaatcgc gccggatatt ttgtgtctcg gtaaagcctt
    32101 aaccggcggc acaatgaccc tttccgccac gctcaccacg cgcgaggttg cggaaaccat
    32161 cagtaacggc gaagccggct gctttatgca tgggccaact tttatgggca atccgctggc
    32221 ctgcgcagca gcaaacgcca gcctggcgat tctcgaatct ggcgactggc agcatcaggt
    32281 ggcggctatt gaagggcagc tacgcgagca acttgccccc gcctgtgacg ccgaaatggt
    32341 tgccgatgtg cgcgtactgg gggcaatcgg cgtggtcgaa accactcgtc cggtgaatat
    32401 ggcagcgctg caaaaattct ttgtcgaaca gggtgtctgg atccggcctt ttggcaaact
    32461 gatttacctg atgccgccct atattattct cccgcaacag ttgcagcgtc tgaccgcagc
    32521 ggttaatcgt gcggtgcagg atgaaacatt tttttgccaa taacgggaag ttagcgtgag
    32581 ggtttctggc tacactttct gcaaacaaga aaggagggct catgaaactc atcagtaatg
    32641 atttacgcga tggcgataag ttgccacatc gtcatgtctt taacggcatg ggttacgatg
    32701 gcgataatat ttcaccgcat ctggcgtggg atgatgttcc tgtgggaacg aaaagttttg
    32761 ttgtcacctg ctacgacccg gatgcgccaa ccggctccgg ctggtggcac tgggtagttg
    32821 ttaatttacc cgctgatacc cgtgtattac cgcaagggtt tggctctggt ctggtagcaa
    32881 tgccagacgg cgttttgcag acgcgtaccg actttggtaa aaccgggtac gatggtgcag
    32941 cgccgccgaa aggcgaaacc catcgctaca tttttaccgt tcacgcgctg gatgtagaac
    33001 gtattgatgt cgatgaaggt gccagcggcg cgatggtcgg gtttaacgtt catttccact
    33061 ctctggcgag tgcctcgatt actgcgatgt ttagttaatc actctgccag atggcgcaat
    33121 gccatctggt atcacttaaa ggtattaaaa acaacttttt gtctttttac cttcccgttt
    33181 cgctcaagtt agtataaaaa agctgaacgt gaaacattaa aagccattaa tatcaatgca
    33241 ttacaatatt tttagtctaa aaaatagact acataatgct acaaaacaca acatacccag
    33301 tcactatgaa tcaaccactt agatggtatt agtgacctgt aacagagcat tagcgcaagg
    33361 tgactttttg tcttcttgcg ctaatttttt gtcaaacaca ttacgtcgtt tgctgaattt
    33421 acaaccatgc tcagtatctc gataagcgca gagaaatgat gcagtggtgg gcggattggc
    33481 ttgatgaaaa ggtgtagcaa ccaacattgc tatatctcag atagcacaaa gccttgcaat
    33541 ccagtgcaaa gctttgtgtg tctcagtttt gtctcatcgg taattatgtg ttcgctacga
    33601 tgcaagaatc cataccaata agcatccgat aattgggaaa gaaaaatcca acaaacttcc
    33661 cacatcccac acgcgtggat caaaacctcc ccaccacggc atgttga
//
LOCUS       DAMZFT010000043        33677 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.043, whole genome shotgun sequence.
ACCESSION   DAMZFT010000043 DAMZFT010000000
VERSION     DAMZFT010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 33677)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33677)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33677
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.043"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(<1..302)
                     /locus_tag="PN977_002122"
     CDS             complement(<1..302)
                     /locus_tag="PN977_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HDI6042151.1"
                     /translation="MQRRDFLKYSVALGVASALPLWSRAVFAAERPTLPIPDLLTTDA
                     RNRIQLTIGAGQSTFGGKTATTWGYNGNLLGPAVKLQRGKAVTVDIYNQLTEETTL"
     gene            468..815
                     /locus_tag="PN977_002123"
     CDS             468..815
                     /locus_tag="PN977_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YacC family pilotin-like protein"
                     /protein_id="HDI6042152.1"
                     /translation="MKTFFRTVLFGSLMAVCANSYALSESEAEDMADLTAVFVFLKND
                     CGYQNLPNGQIRRALVFFAQQNQWDLSNYDTFDMKALGEDSYRDLSGIGIPVAKKCKA
                     LARDSLSLLAYVK"
     gene            921..1787
                     /gene="speE"
                     /locus_tag="PN977_002124"
     CDS             921..1787
                     /gene="speE"
                     /locus_tag="PN977_002124"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HDI6042153.1"
                     /translation="MAEKKQWHETLHDQFGQYFAVDNVLYHEKTDHQDLIIFENAAFG
                     RVMALDGVVQTTERDEFIYHEMMTHVPLLAHGHAKHVLIIGGGDGAMLREVTRHKNVE
                     SITMVEIDAGVVSFCRQYLPNHNAGSYDDPRFKLVIDDGVNFVNQTSQTFDVIISDCT
                     DPIGPGESLFTSAFYEGCKRCLNPGGIFVAQNGVCFLQQEEAIDSHRKLSHYFSDVGF
                     YQAAIPTYYGGIMTFAWATDNDALRHLSTEIIQARFLASGLKCRYYNPAIHTAAFALP
                     QYLQDALASQPS"
     gene            1803..2597
                     /gene="speD"
                     /locus_tag="PN977_002125"
     CDS             1803..2597
                     /gene="speD"
                     /locus_tag="PN977_002125"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HDI6042154.1"
                     /translation="MKKLKLHGFNNLTKSLSFCIYDICYAKTAEERDGYIAYIDELYN
                     ANRLTEILSETCSIIGANILNIARQDYEPQGASVTILVSEEPVDPKLIDKTEHPGPLP
                     ETVVAHLDKSHICVHTYPESHPEGGLCTFRADIEVSTCGVISPLKALNYLIHQLESDI
                     VTIDYRVRGFTRDINGMKHFIDHEINSIQNFMSDDMKALYDMVDVNVYQENIFHTKML
                     LKEFDLKHYMFHTKPEDLTDSERQEITAALWKEMREIYYGRNMPAV"
     gene            complement(2635..2997)
                     /locus_tag="PN977_002126"
     CDS             complement(2635..2997)
                     /locus_tag="PN977_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YacL family protein"
                     /protein_id="HDI6042155.1"
                     /translation="MDYEFLRDVTGVVKVRMSMGHEVIGHWFNEEVKENLALLDEVED
                     AARTLKGSERSWQRAGHEYTLWMDGEEVMVRANQLEFAGDEMEEGMNYYDEESLSLCG
                     VEDFLQVVAAYRNFVQQK"
     gene            complement(3172..5769)
                     /gene="acnB"
                     /locus_tag="PN977_002127"
     CDS             complement(3172..5769)
                     /gene="acnB"
                     /locus_tag="PN977_002127"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aconitate hydratase
                     2/2-methylisocitrate dehydratase"
                     /protein_id="HDI6042156.1"
                     /translation="MLEEYRKHVAERAAEGIAPKPLDANQMAALVELLKNPPAGEEEF
                     LLDLLTNRVPPGVDEAAYVKAGFLAAVAKGEAKSPLLTPEKAIELLGTMQGGYNIHPL
                     IDALDDAKLAPIAAKALSHTLLMFDNFYDVEEKAKAGNEYAKQVMQSWADAEWFLNRP
                     ALAEKLTVTVFKVTGETNTDDLSPAPDAWSRPDIPLHALAMLKNAREGIEPDQPGVVG
                     PIKQIEALQQKGFPLAYVGDVVGTGSSRKSATNSVLWFMGDDIPHVPNKRGGGLCLGG
                     KIAPIFFNTMEDAGALPIEVDVSNLNMGDVIDVYPYKGEVRNHETGELLATFELKTDV
                     LIDEVRAGGRIPLIIGRGLTTKAREALGLPHSDVFRQAKDVAESDRGFSLAQKMVGRA
                     CGVKGIRPGAYCEPKMTSVGSQDTTGPMTRDELKDLACLGFSADLVMQSFCHTAAYPK
                     PVDVNTHHTLPDFIMNRGGVSLRPGDGVIHSWLNRMLLPDTVGTGGDSHTRFPIGISF
                     PAGSGLVAFAAATGVMPLDMPESVLVRFKGKMQPGITLRDLVHAIPLYAIKQGLLTVE
                     KKGKKNIFSGRILEIEGLPDLKVEQAFELTDASAERSAAGCTIKLNKEPIIEYLNSNI
                     VLLKWMIAEGYGDRRTLERRIQGMEKWLANPELLEADADAEYAAVIDIDLADIKEPIL
                     CAPNDPDDARPLSAVQGEKIDEVFIGSCMTNIGHFRAAGKLLDAHKGQLPTRLWVAPP
                     TRMDAAQLTEEGYYSVFGKSGARIEIPGCSLCMGNQARVADGATVVSTSTRNFPNRLG
                     TGANVFLASAELAAVAALIGKLPTPEEYQTYVAQVDKTAVDTYRYLNFNQLSQYTEKA
                     DGVIFQTAV"
     gene            6124..7881
                     /locus_tag="PN977_002128"
     CDS             6124..7881
                     /locus_tag="PN977_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3300 domain-containing protein"
                     /protein_id="HDI6042157.1"
                     /translation="MKMTLPFKPHVLALICSAGLCAASAGLYIKSRTVEAPVESQSTQ
                     QTAPDISAVTLPATVSAPPVTPAVVKSTFSTAQIDQWVAPVALYPDSLLSQVLMASTY
                     PANVAQAVQWSHDNPLKQGDAAIQAVSDQPWDASVKSLVAFPQLMALMGENPQWVQNL
                     GDAFLAQPQDVMDSVQRLRQLAQQTGSLKSSTEQKIITTTKKVVPVNQPANAPVTQSN
                     TVSTSSPVVAEPAPTVITIEPANPDVVYIPNYNPNVVYGSWANTAYPPVYLPPPAGEP
                     FVDSFVRGFGYSMGVATTYALFSSIDWDDDDHDHHHHDDDDYHHHDGGHRDGNDWQHN
                     GDNINIDVNNFNRITGEHLTDKNMAWRHNPNYRDGVPYHDQDMAKRFHQTDVNGGMSA
                     TQLPALSRDSQRQAAASQFQQRTHTAPVITRDTQRQAAAQRFNEAEHYGSYDDFREFS
                     RRQPLTQQQKDAARQRYQSASPEQRQAVREKMQTNPQNQQRREVARERIQSATPEQRQ
                     VFKEKVQQRPLNQQQRDNARQRIQSASPEQRQVFREKVQESRPQRLNDSNHTVRLNNE
                     QRSAVRERLSERGARRLER"
     gene            complement(7952..9376)
                     /gene="lpdA"
                     /locus_tag="PN977_002129"
     CDS             complement(7952..9376)
                     /gene="lpdA"
                     /locus_tag="PN977_002129"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HDI6042158.1"
                     /translation="MSTEIKTQVVVLGAGPAGYSAAFRCADLGLETVIVERYNTLGGV
                     CLNVGCIPSKALLHVAKVIEEAKALAEHGIVFGEPKTDIDKIRTWKEKVINQLTGGLA
                     GMAKGRKVKVVNGLGKFTGANTLEVEGENGKTVINFDNAIIAAGSRPIQLPFIPHEDP
                     RIWDSTDALELKEVPERLLVMGGGIIGLEMGTVYHALGSQIDVVEMFDQVIPAADKDI
                     VKVFTKRISKKFNLMLETKVTAVEAKEDGIYVTMEGKKAPAEPQRYDAVLVAIGRVPN
                     GKNLDAGKAGVEVDDRGFIRVDKQLRTNVPHIFAIGDIVGQPMLAHKGVHEGHVAAEV
                     IAGKKHYFDPKVIPSIAYTEPEVAWVGLTEKEAKEKGISYETATFPWAASGRAIASDC
                     ADGMTKLIFDKESHRVIGGAIVGTNGGELLGEIGLAIEMGCDAEDIALTIHAHPTLHE
                     SVGLAAEVFEGSITDLPNPKAKKK"
     gene            complement(9584..11476)
                     /gene="aceF"
                     /locus_tag="PN977_002130"
     CDS             complement(9584..11476)
                     /gene="aceF"
                     /locus_tag="PN977_002130"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex
                     dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HDI6042159.1"
                     /translation="MAIEIKVPDIGADEVEITEILVKVGDKVEAEQSLITVEGDKASM
                     EVPSPQAGIVKEIKVSVGDKTQTGALIMIFDSADGAADAAPAQAEEKKEAAPAAAPAA
                     AAAKDVNVPDIGSDEVEVTEILVKVGDKVEAEQSLITVEGDKASMEVPAPFAGTVKEI
                     KVNVGDKVSTGSLIMVFEVAGEAGAAAPAAKQEAAPAAAPASAAGVKDVNVPDIGGDE
                     VEVTEVMVKVGDKVAAEQSLITVEGDKASMEVPAPFAGVVKELKVNVGDKVKTGSLIM
                     IFEVEGAAPAAAPAKQEAAAPAPAAKAEAPAAAPAAKAEGKSEFAENDAYVHATPLIR
                     RLAREFGVNLAKVKGTGRKGRILREDVQAYVKEAIKRAEAAPAATGGGIPGMLPWPKV
                     DFSKFGEIEEVELGRIQKISGANLSRNWVMIPHVTHFDKTDITELEAFRKQQNEEAAK
                     RKLDVKITPVVFIMKAVAAALEQMPRFNSSLSEDGQRLTLKKYINIGVAVDTPNGLVV
                     PVFKDVNKKGIIELSRELMTISKKARDGKLTAGEMQGGCFTISSIGGLGTTHFAPIVN
                     APEVAILGVSKSAMEPVWNGKEFVPRLMLPISLSFDHRVIDGADGARFITIINNTLSD
                     IRRLVM"
     gene            complement(11491..14154)
                     /gene="aceE"
                     /locus_tag="PN977_002131"
     CDS             complement(11491..14154)
                     /gene="aceE"
                     /locus_tag="PN977_002131"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008814576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HDI6042160.1"
                     /translation="MSERFPNDVDPIETRDWLQAIESVIREEGVERAQYLIDQLLAEA
                     RKGGVNVAAGTGISNYINTIPVEEQPEYPGNLELERRIRSAIRWNAIMTVLRASKKDL
                     ELGGHMASFQSSATIYDVCFNHFFRARNEQDGGDLVYFQGHISPGVYARAFLEGRLTQ
                     EQLDNFRQEVHGNGLSSYPHPKLMPEFWQFPTVSMGLGPIGAIYQAKFLKYLEHRGLK
                     DTSKQTVYAFLGDGEMDEPESKGAITIATREKLDNLVFVINCNLQRLDGPVTGNGKII
                     NELEGIFEGAGWNVIKVMWGSRWDELLRKDTSGKLIQLMNETVDGDYQTFKSKDGAYV
                     REHFFGKYPETAALVADWTDEQIWALNRGGHDPKKIYAAFKKAQETKGKATVILAHTI
                     KGYGMGDAAEGKNIAHQVKKMNMDGVRHIRDRFNVPVSDADIEKLPYITFPEGSEEHT
                     YLHAQRQKLHGYLPSRQPNFTEKLELPSLQDFGALLEEQSKEISTTIAFVRALNVMLK
                     NKSIKDRLVPIIADEARTFGMEGLFRQIGIYSPNGQQYTPQDREQVAYYKEDEKGQIL
                     QEGINELGAGCSWLAAATSYSTNNLPMIPFYIYYSMFGFQRIGDLCWAAGDQQARGFL
                     IGGTSGRTTLNGEGLQHEDGHSHIQSLTIPNCISYDPAYAYEVAVIMHDGLERMYGEK
                     QENVYYYITTLNENYHMPAMPEGAEEGIRKGIYKLETIEGSKGKVQLLGSGSILRHVR
                     EAAEILAKDYGVGSDVYSVTSFTELARDGQDCERWNMLHPLETPRVPYIAQVMNDAPA
                     VASTDYMKLFAEQVRTYVPADDYRVLGTDGFGRSDSRENLRHHFEVDASYVVVAALGE
                     LAKRGEIDKKVVADAIAKFNIDADKVNPRLA"
     gene            complement(14314..15078)
                     /gene="pdhR"
                     /locus_tag="PN977_002132"
     CDS             complement(14314..15078)
                     /gene="pdhR"
                     /locus_tag="PN977_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex transcriptional
                     repressor PdhR"
                     /protein_id="HDI6042161.1"
                     /translation="MAYSKIRQPKLSDVIEQQLEFLILEGTLRPGEKLPPERELAKQF
                     DVSRPSLREAIQRLEAKGLLLRRQGGGTFVQSSLWQSFSDPLVELLSDHPESQYDLLE
                     TRHALEGIAAYYAALRSTDEDKERIRELHHAIELAQQSGDLDAESNAVLQYQIAVTEA
                     AHNVVLLHLLRCMEPMLAQNVRQNFELLYSRREMLPLVSSHRTRIFEAIMAGKPEEAR
                     EASHRHLAFIEEILLDRSREESRRERSLRRLEQRKN"
     gene            complement(15534..15824)
                     /locus_tag="PN977_002133"
     CDS             complement(15534..15824)
                     /locus_tag="PN977_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000543775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HDI6042162.1"
                     /translation="MHDLKYKIYFYTEGEFLEMLEEIVNATSKDKSLKGKKLEKYLDT
                     LVDHFIKITEHPKKGDLIFYPNSPEDGKPENILKIVKEWRRSQGLSLFKDSE"
     gene            complement(15878..16066)
                     /locus_tag="PN977_002134"
     CDS             complement(15878..16066)
                     /locus_tag="PN977_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012311815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HDI6042163.1"
                     /translation="MSQYPELIAQFSTGNQTRIKQGLIAKAPLEGWYYGSKEIVKEFH
                     IYHSVAIECGGEIYDIDN"
     gene            complement(16233..16523)
                     /locus_tag="PN977_002135"
     CDS             complement(16233..16523)
                     /locus_tag="PN977_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000282231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HDI6042164.1"
                     /translation="MYDFKNKIEDYTEREFIELLGEFTNPTGDNAQLKGEELDKYWDD
                     LEEHLTRITQHPLMSDLIYYPAKKGDDEPENILKIVKEWRRSQGLPLFKDSK"
     gene            complement(16524..16763)
                     /locus_tag="PN977_002136"
     CDS             complement(16524..16763)
                     /locus_tag="PN977_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020231867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6042165.1"
                     /translation="MSQYPELIAQFSSGNQTRIKQGLIAKAPLEGWHYGTKEIVKKFH
                     IYHRVAIEYSGGIYDIDNLRRAHLICMMKFITGDS"
     gene            complement(16931..17215)
                     /locus_tag="PN977_002137"
     CDS             complement(16931..17215)
                     /locus_tag="PN977_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000471929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HDI6042166.1"
                     /translation="MFKEKLQDYTEDEFLNFLGGLRSSMKDGKSLKGKELEMYWDSLV
                     DHFIEITQHPSGSDLIFYPKSQGDDKPENILKIVKEWRRSQGLPLFKDSK"
     gene            complement(17218..18999)
                     /gene="hcp"
                     /locus_tag="PN977_002138"
     CDS             complement(17218..18999)
                     /gene="hcp"
                     /locus_tag="PN977_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020238820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tube protein Hcp"
                     /protein_id="HDI6042167.1"
                     /translation="MGDIVYLRIIGEKQGDISSGCGTYASVGNRWQVGHEDEIFAFAL
                     TNTITSTGKGVNLQGLQFCKLIDKSSPLLSNAINQNERLFIEIDLYRINKSGRWERYY
                     YIQLRNASLTAIHVNISDNNLPTECVTVDYDYILCKHLIANTEFDWLAFPAGHNSLFI
                     PPKNPPASNLNPEPLPVVNLPLSPPAVKPVYAKSCLKEKGCTDAGTAEEPAENFGQVA
                     IFALPVVDDCCGYHHPEANDVGQPAEAQTMLLFPGSVLAAQIWGKWSLSGILSATRGS
                     YIGALASALYIPSAGEGSARVPGRDEFWYEEELQQKALAGSTATTRVRFFWGTDIHGK
                     PQVYGVHTGEGTPYENVRVANMQWNEQTQRYEFTPAHDVDGPLITWTPENPEHGNVPG
                     HTGNDRPPLEQPTILVTPIPDGTDTYSTPPFPVPDPKEFNDYILVFPAGSGIKPIYVY
                     LKEDPRKLPGVVTGRGVPLSPGTRWLDMSVSNNGNGAPIPAHIADKLRGREFKTFDEF
                     REALWLEVSQDPELIAQFSSGNQTRIKQGLTAKAPIDGWHYGPKEIVKKFQIHHRVAI
                     EYGGSVYDIDNLRIVTPRLHDEIHYRR"
     gene            19525..20898
                     /gene="aroP"
                     /locus_tag="PN977_002139"
     CDS             19525..20898
                     /gene="aroP"
                     /locus_tag="PN977_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transporter AroP"
                     /protein_id="HDI6042168.1"
                     /translation="MMEGQQHGEQLKRGLKNRHIQLIALGGAIGTGLFLGSASVIQSA
                     GPGIILGYAIAGFIAFLIMRQLGEMVVEEPVAGSFSHFAYKYWGSFAGFASGWNYWVL
                     YVLVAMAELTAVGKYIQFWYPEIPTWVSAAVFFVVINAINLTNVKVFGEMEFWFAIIK
                     VIAVVAMIIFGGWLLFSGNGGPQATVSNLWDQGGFLPHGFTGLVMMMAIIMFSFGGLE
                     LVGITAAEADNPEQSIPKATNQVIYRILIFYIGSLAVLLSLMPWTRVTADTSPFVLIF
                     HELGDTFVANALNIVVLTAALSVYNSCVYCNSRMLFGLAQQGNAPKALASVDKRGVPV
                     NTILVSALVTALCVLINYLAPESAFGLLMALVVSALVINWAMISLAHMKFRRAKQEQG
                     VVTRFPALLYPLGNWICLLFMAAVLVIMLMTPGMAISVYLIPVWLIVLGIGYLFKEKT
                     AKAVKAH"
     gene            complement(20941..21795)
                     /gene="ampE"
                     /locus_tag="PN977_002140"
     CDS             complement(20941..21795)
                     /gene="ampE"
                     /locus_tag="PN977_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase regulator AmpE"
                     /protein_id="HDI6042169.1"
                     /translation="MTLFTTLLVLIFERLFKLGEHWQLDHRLEAFFRRVKHFSLGRTL
                     GMTIIAMGVTFLLLRTLQGVLFNVPTLLVWLLIGLLCIGAGKVRLHYHAYLTAASRND
                     SHARATMAGELTMIHGVPAGCDEREYLRELQNALLWINFRFYLAPLFWLIVGGTWGPV
                     TLMGYAFLRAWQYWLARYQTPHHRLQSGIDGVLHVLDWVPVRLAGVVYALIGHGEKAL
                     PAWFASLGDFHTSQYQVLTRLAQFSLAREPHVDKVETPKAAVSMAKKTSFVVVVVIAL
                     LTIYGALV"
     gene            complement(21792..22343)
                     /gene="ampD"
                     /locus_tag="PN977_002141"
     CDS             complement(21792..22343)
                     /gene="ampD"
                     /locus_tag="PN977_002141"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,6-anhydro-N-acetylmuramyl-L-alanine amidase
                     AmpD"
                     /protein_id="HDI6042170.1"
                     /translation="MLLEQGWLAGARRVPSPHYDCRPDDETPTLLVVHNISLPPGEFG
                     GPWIDALFTGTIDPQAHPFFAEIAHLRVSAHCLIRRDGEIVQYVPFDKRAWHAGVSQY
                     QGRERCNDFSIGIELEGTDTLAYTDAQYQQLAAVTRALIDRYPDIANNMTGHCDIAPD
                     RKTDPGPAFDWARFRALVSKETT"
     gene            22431..23324
                     /gene="nadC"
                     /locus_tag="PN977_002142"
     CDS             22431..23324
                     /gene="nadC"
                     /locus_tag="PN977_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HDI6042171.1"
                     /translation="MPPRRYNPDTRRDELLERINLDIPGAVAQALREDLGGTVDANND
                     ITAKLLPENSRSHATVITRENGVFCGKRWVEEVFIQLAGDDVTIIWHVDDGDVINANQ
                     PLFELEGPSRVLLTGERTALNFVQTLSGVASKVRHYVELLEGTNTQLLDTRKTLPGLR
                     SALKYAVLCGGGANHRLGLSDAFLIKENHIIASGSVRQAVEKASWLHPDAPVEVEVEN
                     LEELDEALKAGADIIMLDNFETEQMREAVKRTNGKALLEVSGNVTDKTLREFAETGVD
                     FISVGALTKHVQALDLSMRFC"
     gene            23527..23967
                     /gene="ppdD"
                     /locus_tag="PN977_002143"
     CDS             23527..23967
                     /gene="ppdD"
                     /locus_tag="PN977_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent pilin"
                     /protein_id="HDI6042172.1"
                     /translation="MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQ
                     TFVPYRTAVELCALEHGGLNTCDGGSNGIPSPTTTRYVSAMSVAKGVVSLTGQESLNG
                     LSVVMTPGWDNANGVTGWTRNCNIQSDSALQQACEDVFRFDDAN"
     gene            23977..25362
                     /gene="gspE"
                     /locus_tag="PN977_002144"
     CDS             23977..25362
                     /gene="gspE"
                     /locus_tag="PN977_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspE"
                     /protein_id="HDI6042173.1"
                     /translation="MNIPQLTALCLRYQGVLLDASEEVVHVAVVDAPSHELLDALHFA
                     TTKRIEITCWTRQQMEGHASRTQQTLPVAVQEKHQPKAELLTRTLQSALEQRASDIHI
                     EPADNAYRIRLRIDGVLHPLPVVSPDAGVALTARLKVLGNLDIAEHRLPQDGQFTVEL
                     AGNAVSFRIATLPCRGGEKVVLRLLQQVSQALDVNTLGMQPLQLADFAHALQQPQGLV
                     LVTGPTGSGKTVTLYSALQTLNTADINICSVEDPVEIPIAGLNQTQIHSRAGLTFQGV
                     LRALLRQDPDVIMIGEIRDGETAEIAIKAAQTGHLVLSTLHTNSTCETLVRLQQMGVA
                     RWMLSSALTLVIAQRLVRKLCPHCRRQQGEPIHIPDNVWPSPLPHWQAPGCVHCYHGF
                     YGRTALFEVLPITPIIRQLISANTDVESLETHARQAGMRTLFENGCLAVEQGLTTFEE
                     LIRVLGMPHGE"
     gene            25352..26554
                     /gene="hofC"
                     /locus_tag="PN977_002145"
     CDS             25352..26554
                     /gene="hofC"
                     /locus_tag="PN977_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein transport protein HofC"
                     /protein_id="HDI6042174.1"
                     /translation="MASKQLWRWHGITGDGNAQDGMLWAESRALLLIALQQQMVTPLS
                     LKRIAINSAQWRGDKSAEVIHQLATLLKAGLTLSEGLALLAEQHPSKQWQALLQSLAY
                     DLEQGIAFSNALLPWSEVFPPLYQAMIRTGELTGKLDECCFELARQQKAQRQLTDKVK
                     SALRYPIIILAMAIMVVVAMLHFVLPEFAAIYKIFNTPLPALTQGIMTLASFSGEWGW
                     LLVLFGFLLAIANKLLMRHPTRLIARQKLLLRIPIMGSLMRGQKLTQIFTILTLTQSA
                     GISFLQGVESVRETMRCPYWVQLLTQIQHDISNGHPIWLALKNAGEFSPLCLQLVRTG
                     EASGSLDLMLDNLAHHHRDNTMALADNLAALLEPALLIITGGIIGTLVVAMYLPIFHL
                     GDAMSGMG"
     gene            complement(26589..27632)
                     /gene="guaC"
                     /locus_tag="PN977_002146"
     CDS             complement(26589..27632)
                     /gene="guaC"
                     /locus_tag="PN977_002146"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008461816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HDI6042175.1"
                     /translation="MRIEEDLKLGFKDVLIRPKRSTLKSRSDVALERQFTFKHSGQSW
                     SGVPIIAANMDTVGTFSMASALASFDILTAVHKHYSVEEWQAFINNSSADVLKHVMVS
                     TGTSDADFEKTKQILDLNPALNFVCIDVANGYSEHFVQFVAKAREAWPTKTICAGNVV
                     TGEMCEELILSGADIVKVGIGPGSVCTTRVKTGVGYPQLSAVIECADAAHGLGGMIVS
                     DGGCTTPGDVAKAFGGGADFVMLGGMLAGHEESGGRIVEENGEKFMLFYGMSSESAMK
                     RHVGCVAEYRAAEGKTVKLPLRGPVENTARDILGGLRSACTYVGASRLKELTKRTTFI
                     RVQEQENRIFNNL"
     gene            complement(27788..27832)
                     /locus_tag="PN977_002147"
     CDS             complement(27788..27832)
                     /locus_tag="PN977_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6042176.1"
                     /translation="MIIKTLKMSARKRN"
     gene            27857..28477
                     /gene="coaE"
                     /locus_tag="PN977_002148"
     CDS             27857..28477
                     /gene="coaE"
                     /locus_tag="PN977_002148"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HDI6042177.1"
                     /translation="MRYIVALTGGIGSGKSTVANAFADLGINVIDADIIARQVVEPGA
                     PALHAIADHFGANMIAADGTLQRRALRERIFANPEEKNWLNALLHPLIQQETQHQIQQ
                     ATSPYVLWVVPLLVENSLYKKANRVLVVDVSPETQLKRTMQRDDVTREHVEQILAAQA
                     TREARLAVADDVIDNNGAPDAIASDVARLHAHYLQLASQFVSQEKP"
     gene            28477..29220
                     /gene="zapD"
                     /locus_tag="PN977_002149"
     CDS             28477..29220
                     /gene="zapD"
                     /locus_tag="PN977_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapD"
                     /protein_id="HDI6042178.1"
                     /translation="MQTQVLFEHPLNEKMRTWLRIEFLIQQLTVNLPIVDHAGALHFF
                     RNVSELLDVFERGEVRTELLKELDRQQRKLQTWIGVPGVDQSRIEALIQQLKAAGSVL
                     ISAPRIGQFLREDRLIALVRQRLSIPGGCCSFDLPTLHIWLHLPQAQRDSQVETWIAS
                     LNPLTQALTMVLDLIRQSAPFRKQTSLNGFYQDNGGDADLLRLNLSLDSQLYPQISGH
                     KSRFAIRFMPLDSENGQVPERLDFELACC"
     gene            29230..29427
                     /gene="yacG"
                     /locus_tag="PN977_002150"
     CDS             29230..29427
                     /gene="yacG"
                     /locus_tag="PN977_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HDI6042179.1"
                     /translation="MSETITVNCPTCGKTVVWGEISPFRPFCSKRCQLIDLGEWAAEE
                     KRIPSSGDLSESDDWSEEPKQ"
     gene            complement(29516..29914)
                     /gene="mutT"
                     /locus_tag="PN977_002151"
     CDS             complement(29516..29914)
                     /gene="mutT"
                     /locus_tag="PN977_002151"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase MutT"
                     /protein_id="HDI6042180.1"
                     /translation="MKKLQIAVGIIRNENNEIFITRRAADAHMANKLEFPGGKIEMSE
                     TPEQAVVRELQEEVGITPQHFSLFEKLEYEFPDRHITLWFWLVESWEGEPWGKEGQPG
                     KWMSLVGLNADEFPPANEPVIAKLKRVYVG"
     gene            complement(29974..32679)
                     /gene="secA"
                     /locus_tag="PN977_002152"
     CDS             complement(29974..32679)
                     /gene="secA"
                     /locus_tag="PN977_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HDI6042181.1"
                     /translation="MLIKLLTKVFGSRNDRTLRRMRKVVNIINAMEPEMEKLSDEELK
                     GKTAEFRARLEKGEVLENLIPEAFAVVREASKRVFGMRHFDVQLLGGMVLNERCIAEM
                     RTGEGKTLTATLPAYLNALTGKGVHVVTVNDYLAQRDAENNRPLFEFLGLTVGINLPG
                     MPAPAKREAYAADITYGTNNEYGFDYLRDNMAFSPEERVQRKLHYALVDEVDSILIDE
                     ARTPLIISGPAEDSSEMYKRVNKIIPHLIRQEKEDSETFQGEGHFSVDEKSRQVNLTE
                     RGLVLIEELLVKEGIMDEGESLYSPANIMLMHHVTAALRAHALFTRDVDYIVKDGEVI
                     IVDEHTGRTMQGRRWSDGLHQAVEAKEGVQIQNENQTLASITFQNYFRLYEKLAGMTG
                     TADTEAFEFSSIYKLDTVVVPTNRPMIRKDLPDLVYMTEAEKIQAIIEDIKERTAKGQ
                     PVLVGTISIEKSELVSNELTKAGIKHNVLNAKFHANEAAIVAQAGYPAAVTIATNMAG
                     RGTDIVLGGSWQAEVAALENPTAEQIEKIKADWQVRHDAVLEAGGLHIIGTERHESRR
                     IDNQLRGRSGRQGDAGSSRFYLSMEDALMRIFASDRVSGMMRKLGMKPGEAIEHPWVT
                     KAIANAQRKVESRNFDIRKQLLEYDDVANDQRRAIYSQRNELLDVSDVSETINSIRED
                     VFKATIDAYIPPQSLEEMWDIPGLQERLKNDFDLDLPIAEWLDKEPELHEETLRERIL
                     AQSIEVYQRKEEVVGAEMMRHFEKGVMLQTLDSLWKEHLAAMDYLRQGIHLRGYAQKD
                     PKQEYKRESFSMFAAMLESLKYEVISTLSKVQVRMPEEVEELEQQRRMEAERLAQMQQ
                     LSHQDDDSAAAAALAAQTGERKVGRNDPCPCGSGKKYKQCHGRLQ"
     gene            complement(32741..33253)
                     /gene="secM"
                     /locus_tag="PN977_002153"
     CDS             complement(32741..33253)
                     /gene="secM"
                     /locus_tag="PN977_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308128.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secA regulator SecM"
                     /protein_id="HDI6042182.1"
                     /translation="MSGILTRWRQFGKRYFWPHLLLGMVAASLGLPALSNAAEPNAPA
                     KATTRNHEPSAKVNFGQLALLEANTRRPNSNYSVDYWHQHAIRTVIRHLSFAMAPQTL
                     PVAEESLPLQAQHLALLDTLSALLTQEGTPSGKGYRIDYAHFTPQAKFSTPVWINQAQ
                     GIRAGPQRLS"
     gene            complement(33484..>33677)
                     /locus_tag="PN977_002154"
     CDS             complement(33484..>33677)
                     /locus_tag="PN977_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HDI6042183.1"
                     /translation="AIGDLFMCGHNIIGAFTAYKSGHALNNKLLQAVLAKQEAWEYVT
                     FQDDAELPLAFKAPSAVLA"
BASE COUNT         8122 a         8862 c         8190 g         8503 t
ORIGIN      
        1 tgcagcgtcg tctcttccgt cagttggttg tagatatcaa ccgttaccgc tttgccgcgc
       61 tgtaatttca ccgccggccc cagcagattg ccgttatagc cccaggtagt tgcggttttc
      121 ccgccaaagg tggactgacc tgcgccaata gttaactgaa tgcgattacg ggcatcggtc
      181 gtgagcaaat cagggatcgg taacgttggg cgttctgccg caaatactgc gcggctccac
      241 agcggcaaag ccgaagccac acccagcgcg acggaatatt ttaagaaatc acgacgttgc
      301 atagttattt ccttattctt aagcaggcga aacaaaatca aacgttgagc atagtccttc
      361 cccttacggg aaggtcaagc cgcagacaca atttaatcga caataatggt cgtcgcctcg
      421 ctcgcagtgt gctaacgttt atcttcttta agcccctggt agaagcaatg aagacgtttt
      481 tcagaacagt gttattcggc agcctgatgg ccgtttgcgc aaacagttac gcgctcagcg
      541 agtctgaagc cgaagatatg gccgatttaa cggcagtttt tgtctttctg aagaacgatt
      601 gtggttacca gaacttacct aacgggcaaa ttcgtcgcgc actggtcttt ttcgcccagc
      661 aaaaccagtg ggatctcagt aattacgaca ccttcgacat gaaagccctc ggtgaagaca
      721 gctaccgcga tctcagcggc attggcattc ccgtcgctaa aaaatgcaaa gccctggccc
      781 gcgattcctt aagcctgctt gcctacgtca aataatccct gatacctttt tgtagaaata
      841 ttgaccgtgc atccgcggtc aatgttagct attatgttgc gccctttttt tacgggtgtt
      901 aacaaaggag gtatcaaccc atggccgaaa aaaaacagtg gcatgaaacg ctacacgacc
      961 agtttgggca gtactttgcg gtagataacg ttctgtatca tgaaaagacc gatcaccagg
     1021 atctgatcat ttttgagaac gctgcatttg gtcgcgtaat ggcgctggat ggcgtagtac
     1081 aaaccaccga gcgcgacgag tttatctatc atgagatgat gacccatgtt ccgctactgg
     1141 cccacggtca cgcgaaacat gtgctgatta tcggcggcgg cgacggtgcc atgctgcgtg
     1201 aagtaacccg acataaaaac gttgagtcaa tcacgatggt ggaaatcgat gcgggtgtcg
     1261 tatcgttctg ccgtcagtat ctgcccaacc ataacgccgg tagctacgac gatccgcgct
     1321 ttaaactggt gatcgacgat ggcgtcaatt tcgtgaatca aaccagccag acctttgatg
     1381 tcattatctc cgactgcacc gatcctatcg gtcccggcga aagtctgttc acctcagcat
     1441 tttatgaagg ctgcaaacgt tgcctgaatc caggcggtat tttcgtcgca caaaacggcg
     1501 tctgcttttt acagcaggaa gaagccatcg acagccatcg caaactcagc cattacttca
     1561 gcgacgttgg cttttatcag gcggcgatcc cgacctatta cggcggcatc atgacctttg
     1621 catgggcgac agataacgat gccttacgcc atctctcaac cgaaattatt caggcgcgtt
     1681 ttctcgcttc tggcctgaaa tgccgttatt acaatccggc aatccatacg gcagcttttg
     1741 ccttacctca gtatctgcaa gacgcactgg cttcacagcc gtcctaagga gaagataaga
     1801 aattgaaaaa actgaaactg catggcttta ataatctgac caaaagtctg agtttttgta
     1861 tttacgatat ctgctacgcc aaaactgccg aagaacgcga cggttatatt gcttatatcg
     1921 atgaactcta taatgccaac cgtctgaccg aaatcctgtc agaaacctgt tccattatcg
     1981 gggccaatat tcttaacatc gcccgccagg attacgaacc acagggtgcc agcgtcacta
     2041 ttctggtgag tgaagaaccg gttgacccga aacttatcga caaaacagaa caccccggcc
     2101 cactgccaga aacggtcgtt gcccatctcg ataaaagtca tatttgcgta catacctacc
     2161 cggaaagtca tcctgaaggc ggtttatgta ccttccgcgc cgatattgaa gtctccacct
     2221 gcggcgtgat ttccccgctg aaggcgctga attacctgat ccaccagctt gagtccgata
     2281 tcgtaaccat tgattatcgc gtgcgcggtt ttacccgcga cattaacggt atgaagcact
     2341 ttatcgacca tgagattaat tcgattcaga actttatgtc tgacgatatg aaggcgctgt
     2401 atgacatggt ggatgtgaac gtctatcagg agaatatctt ccataccaag atgttgctta
     2461 aagagttcga ccttaagcac tacatgttcc acaccaaacc ggaagattta accgacagcg
     2521 agcgccagga aattaccgct gcgctgtgga aagaaatgcg cgagatttat tacgggcgca
     2581 atatgcccgc tgtttaacgt ctcaggcgga gcacggaggc tccgcctgat ttacttactt
     2641 ctgctggacg aaattacggt aagccgccac cacctgaaga aaatcctcaa cgccgcatag
     2701 cgacaagctc tcttcatcgt agtagttcat cccctcttcc atttcatcgc cagcgaattc
     2761 cagttgattg gcgcgaacca tcacttcttc gccgtccatc cacagggtgt attcatgccc
     2821 tgcccgctgc caggaacgtt cgctgccttt gagcgtgcgc gctgcgtctt ccacttcatc
     2881 aagcaaggcc aggttttctt tcacctcttc attaaaccag tgcccgatga cttcatgccc
     2941 catggacatg cgcaccttta ccactccggt tacatcacgc agaaattcgt aatccatagt
     3001 gtgttcctct gctcccggca atgcgccgaa atcgcattgc gccattgatg taattatcgc
     3061 agccttaccg gagaaaaaca gcggaacggc gatggtttag aaataaaaaa tgcccgggaa
     3121 tctcattcac cgggcattgt gtcgtttatg cgcagcgcgt gcgcttactt attaaaccgc
     3181 agtctggaaa atcaccccat cggctttttc ggtgtactga gaaagctggt tgaagttcag
     3241 ataacggtaa gtatcaacgg cggttttatc tacttgcgcc acgtaggtct ggtactcttc
     3301 cggcgtcggc agtttgccaa tcagcgccgc aacagcagcc agttcagcag aagccaggaa
     3361 gacattcgcg ccagtaccta aacggttcgg gaagttacgg gtagaggtgg aaaccaccgt
     3421 cgcaccgtct gctacacgcg cctggttacc catacacagg gaacagccag ggatctcgat
     3481 acgagcacca ctcttaccga agacgctgta gtagccttct tcggtcagtt gcgcggcgtc
     3541 catacgcgtt ggcggtgcca cccacaggcg ggtcggcagt tgacctttgt gcgcatccag
     3601 cagtttacca gcagcacgga agtgaccgat gttagtcatg caagatccga taaatacttc
     3661 gtcgatcttc tcgccctgta ccgcagacag cggacgtgcg tcgtccgggt cattcggtgc
     3721 acagaggatt ggctctttga tatccgccag atcgatgtcg atcactgccg cgtattccgc
     3781 atctgcatcg gcttccagca gctcaggatt cgccagccat ttttccatgc cctgaatacg
     3841 gcgttccagg gtacgacgat cgccgtaacc ttccgcgatc atccacttca gcaggacgat
     3901 gttagagttc aggtattcga tgatcggttc tttgttcagc ttgatagtac aaccagcggc
     3961 agaacgctca gcggacgcat cggtcagctc aaaggcctgc tcaactttca gatccggcag
     4021 accttcaatt tccagaatgc gaccagagaa gatgtttttc ttgcctttct tctcaacggt
     4081 cagcagacct tgtttgattg catacagcgg aatggcgtga accaggtcgc gcagggtgat
     4141 gcccggctgc attttgcctt tgaagcgcac cagaacggat tccggcatat ccagcggcat
     4201 tacgcctgtt gcggctgcaa atgccaccag accagaaccc gccgggaaag agataccgat
     4261 cgggaaacgg gtatgggaat caccaccggt accgacggta tccggcagca gcatacggtt
     4321 cagccaggag tggattacgc cgtcacccgg acgcagcgac acaccgccac ggttcataat
     4381 gaagtccggc agcgtgtggt gcgtattcac gtcaactggc ttcggatacg ccgcggtgtg
     4441 gcagaaagac tgcatcacca ggtcagccga gaagcccagg cacgccaggt ctttcagttc
     4501 atcacgggtc atcgggccag tggtgtcctg agaaccaaca gaagtcattt tcggttcgca
     4561 gtacgcgccc ggacgaatgc ctttcacacc gcaggcacga ccgaccattt tctgcgccag
     4621 cgagaagccg cgatcgctct cagcgacatc tttcgcctga cggaacacat cgctgtgcgg
     4681 cagaccaagt gcttcacgcg ctttggtggt caggccacgc ccgataatca gcggaatacg
     4741 accaccggca cgcacttcat caatcagcac gtcggttttc agttcgaagg tcgccagcag
     4801 ttcgccggtt tcgtggttac gcacttcacc tttgtacggg taaacgtcaa ttacgtcacc
     4861 catgttcagg ttagagacgt ccacttcaat tggcagtgca cccgcatctt ccatcgtgtt
     4921 aaagaagatg ggtgcaattt taccgccgag gcacaaacca ccgccgcgtt tgttcggcac
     4981 atgtggaata tcatcgccca taaaccacag aacggagttc gtggcggatt tacgcgatga
     5041 acccgtaccc acaacgtcac cgacgtacgc cagcgggaaa cctttctgtt gcagagcttc
     5101 aatctgtttg atcggaccaa caacaccagg ctgatctggc tcaatacctt cacgggcgtt
     5161 tttcagcatc gccagcgcgt gcagtgggat atccgggcgt gaccacgcat ccggtgccgg
     5221 agaaaggtca tcggtgttgg tttcgcctgt gactttgaag acggtaacgg tcagtttttc
     5281 agccagtgcc gggcgattca ggaaccattc ggcatctgcc caggactgca ttacctgctt
     5341 cgcatattcg ttacctgctt tggctttctc ttctacgtca tagaagttat cgaacatcag
     5401 cagcgtgtga gaaagtgctt tggcagcgat cggtgccagt ttggcatcat ccagcgcatc
     5461 gatcagcgga tgaatgttgt aaccaccctg catggtgccc agcagttcga tggctttttc
     5521 cggagtcagc agtggggatt tggcttcgcc tttcgcgaca gcagccagga agcctgcttt
     5581 gacataggcg gcttcatcga cgccaggggg aacacggttg gttaacagat ctaacaggaa
     5641 ttcttcttcg cccgcgggcg ggtttttcag cagctctaca agtgcggcca tttggtttgc
     5701 atccaggggt ttgggcgcaa tcccctcagc ggcacgctca gctacgtgct tacggtattc
     5761 ttctagcacg acggttctcc tcgctctcat tgtcatagtg cggcaggcga ttctcttcac
     5821 gctcctgtga gacagcagtt tgtagggtaa atgcccagta ccgcaacggg cagaatagca
     5881 ggattttgac gatgtgttaa tctgtttaca aaaaagcaac ataaaaaagt ggctgaatcg
     5941 ttaaggatag tctagagaat gtttccctcc gtaaaaactt cgttaattcg catggtgata
     6001 atcacctttc tcaacgaatc ccaagattag tcaaaattta aactacggcc tctttatact
     6061 cggattcaca gcacctgcgg gtggcagttc gccgaccatt gcgatttcct tgagatccga
     6121 attatgaaaa tgactttgcc gtttaaaccc catgtgctgg cgctaatttg cagtgccggg
     6181 ctttgtgccg cctctgccgg gctatatata aaaagccgca cagtggaagc gcctgtggaa
     6241 tcgcaatcga cacaacagac tgcgcctgac atctccgcag ttacgcttcc tgcaacggtt
     6301 tccgcgcctc ccgtaacgcc tgccgtcgtc aaatctacat tcagcactgc acaaatagat
     6361 caatgggttg cgcctgtcgc gctgtacccc gactctctgc tttcacaagt gttaatggca
     6421 tcaacctatc cggcaaacgt tgcgcaagca gtgcaatggt cgcacgataa tccacttaaa
     6481 caaggcgatg ctgctattca ggcggtatct gaccagccct gggacgccag cgtcaaatca
     6541 ctggtggcct ttccacagtt gatggcattg atgggcgaaa acccgcaatg ggtgcaaaac
     6601 ctgggcgatg cttttctggc gcagccgcag gacgtgatgg actcggtaca acgattgcgg
     6661 caactggcgc aacaaaccgg ttcgctaaag tcatcaactg aacaaaagat catcacgaca
     6721 acgaaaaaag tcgtaccggt aaatcagccc gccaacgccc ctgttacaca atcaaatacc
     6781 gtttctacgt ccagccccgt cgtcgcagaa cctgcaccga ccgtaataac cattgagcca
     6841 gccaatccgg atgtggttta tattccgaac tacaacccaa acgtggttta cggcagttgg
     6901 gccaataccg cttatccgcc agtttatctg ccgccgccag ccggagaacc gtttgttgac
     6961 agctttgtgc gcggattcgg ctatagcatg ggcgttgcta ccacatacgc actattcagc
     7021 agcatcgact gggacgacga cgatcatgac catcatcatc atgacgatga tgattatcat
     7081 caccacgatg gcggtcatcg tgacggtaat gactggcaac acaacggcga caacatcaat
     7141 atcgacgtca acaatttcaa ccgtatcact ggtgagcatc ttactgataa gaatatggca
     7201 tggcggcaca atccaaacta ccgtgatggt gtgccctatc atgatcagga tatggcaaag
     7261 cggtttcacc aaaccgatgt caacggcggc atgagcgcca cgcagttacc tgctctatcg
     7321 cgcgacagtc agcgtcaggc agcagcaagt cagtttcagc aacgaacaca cactgcgcca
     7381 gtcattacgc gagatactca acgtcaggct gcggcacagc gatttaatga agcggaacat
     7441 tatgggagct atgacgactt ccgcgagttc agccgtcgcc aaccactgac ccagcaacaa
     7501 aaggacgctg ctcgtcagcg ttatcagtcg gcctcgcctg agcagcgcca ggcagtccgc
     7561 gagaaaatgc agactaaccc gcagaaccag cagcgaagag aggtagcgcg tgagcgtatt
     7621 cagtctgcaa ctcccgaaca gcgccaggtg tttaaggaaa aagtacagca gcgcccactg
     7681 aaccaacagc aacgtgataa cgcccgccag cgtatacaat cggcatcacc tgaacaacgt
     7741 caggtttttc gggagaaagt tcaggagagc cgcccacaac gtctaaacga cagtaaccat
     7801 actgtcaggc tgaataacga gcaacggtca gcagtacgcg aacgtctctc tgagcgcgga
     7861 gcaaggcgac tggaaaggta aattacagac gtaaaaaaag cggcgtggct agccgctttt
     7921 ttaattgccg gatgttccgg caaacgaaaa attacttctt cttcgctttc gggttcggca
     7981 ggtcggtaat gctaccttcg aacacttctg ccgccaggcc cacagactcg tgcagagtcg
     8041 ggtgcgcgtg gatggtcagt gcgatgtctt cagcatcaca acccatttcg attgccagac
     8101 cgatttcacc cagcagctcg ccgccgttag taccgacaat cgcaccaccg atcacacggt
     8161 gagattcttt gtcgaaaatc agcttggtca taccatctgc gcagtcagaa gcgatagctc
     8221 gaccagaagc agcccacggg aaggtggcgg tttcgtagct gatgcctttc tctttcgctt
     8281 ctttctcagt cagacccacc catgcaactt ctggttcggt ataggcgata gacgggataa
     8341 ctttcggatc gaagtagtgt ttcttaccgg caataacttc agcggcaaca tgaccttcgt
     8401 gaacaccttt gtgtgccagc atcggctgac cgacgatatc gccgatagca aagatgtgcg
     8461 gtacgttggt acgcagctgt ttgtcaacgc ggatgaaacc acggtcgtcc acttccacgc
     8521 cagctttgcc tgcgtcgagg tttttaccgt tcggcacacg accaatcgct accagcacgg
     8581 cgtcgtaacg ctgcggttca gcgggtgctt ttttgccttc catcgtcaca taaataccgt
     8641 cttctttcgc ttcaacggcg gtaactttgg tttccagcat caggttgaat ttcttgctga
     8701 tacgcttggt gaagacttta acgatgtctt tatcagctgc cgggatgacc tggtcgaaca
     8761 tttcaaccac gtcaatctgt gaacccagcg cgtggtatac ggtgcccatt tccagaccga
     8821 tgataccgcc acccattacc agcaggcgtt ctggtacttc tttcagttcc agcgcgtcag
     8881 tggagtccca gatacgcgga tcttcatgcg gaataaacgg cagttggatc gggcgagaac
     8941 ccgctgcaat gatcgcgttg tcgaagttga tcacggtttt gccgttctca ccttcaactt
     9001 ccagggtgtt agccccggta aatttaccca gaccgttgac cactttgact ttgcggcctt
     9061 tcgccatacc agccagacca ccggtcagct gattgatcac tttctctttc caggtacgaa
     9121 tcttgtcgat atcggttttc ggttcgccga agacgatacc gtgttcagcc agcgctttgg
     9181 cttcttcgat aacttttgct acgtgcagca gtgctttaga agggatacag ccgacgttca
     9241 ggcaaacacc gccaagggtg ttgtaacgtt ctacgattac ggtttccaga cctaaatcag
     9301 cgcaacggaa ggcagcggag taacctgcgg ggcctgcccc aagtaccacg acctgagttt
     9361 tgatttcagt actcatcatg acctctatat atttatctcc ggcgggtctg acgttttttt
     9421 tatgacgccc attatccacc gggtcgttct atccgtcggt attttacaaa attgttaaca
     9481 atttttaaac aacaaacggc aaccgatttg tctattcgct aataacctca atacattcat
     9541 gagattacca gaaaaaagcc ggccgttggg ccggctcttt tacttacatc accagacggc
     9601 gaatgtcaga cagcgtgttg ttaatgatgg taatgaaacg ggcaccatca gcaccgtcga
     9661 tcacgcggtg gtcgaaggag agagaaatcg gcagcatcag acgcggcacg aactctttac
     9721 cattccacac cggctccatc gcggacttgg aaacgccgag gatagccact tccggcgcgt
     9781 tcacaatcgg cgcgaagtgg gtagtaccca ggccgccgat gctggagatg gtgaagcaac
     9841 cgccctgcat ttcgcccgca gtcagcttac cgtcacgcgc tttcttagaa atagtcatca
     9901 gctcgcgaga cagctcgatg atgcctttct tgttgacgtc tttgaatacc ggaacaacca
     9961 gaccgttcgg ggtatccacc gccacaccga tgttgatgta tttcttcagg gtcagacgct
    10021 gaccgtcttc cgacagcgaa ctgttgaagc gaggcatctg ctccagtgcc gcagcaacgg
    10081 ctttcatgat gaagacaact ggggttatct tcacatccag cttacgtttc gccgcttctt
    10141 cgttctgctg tttacggaac gcttccagct cggtgatatc ggttttgtcg aagtgagtaa
    10201 catgcgggat catcacccag ttacggctca ggttcgcacc agagattttc tggatacggc
    10261 ccagttccac ttcttcgatt tcaccaaact tgctgaagtc caccttcggc cacggcagca
    10321 tgcccgggat accgccgcca gtcgccgccg gagccgcttc tgcacgtttg atagcttctt
    10381 tcacgtaagc ctgaacgtct tcgcgcagga tacgaccttt acggccagtg cccttcactt
    10441 tcgccaggtt aacgccaaac tcgcgtgcca gacggcggat cagcggagtc gcgtgaacgt
    10501 aagcgtcatt ttcagcaaat tcagatttgc cttccgcttt cgcagccgga gctgctgccg
    10561 gagcttcagc ttttgctgcc ggagccggcg ctgccgcttc ctgtttcgca ggagctgccg
    10621 caggcgctgc gccttcaact tcgaagatca taatcagcga gccagttttc actttatcgc
    10681 caacgttgac tttcagttcc ttcacgacgc ctgcaaacgg agccggaact tccatagaag
    10741 ctttgtcgcc ttctacggtg atcagtgact gttcagcggc aactttgtcg cccactttca
    10801 ccatcacttc ggtcacttca acttcgtcac caccgatatc cggaacgtta acgtctttca
    10861 cgccagccgc tgatgcaggg gccgctgccg gagccgcttc ctgtttagcc gccggagctg
    10921 ccgcgcctgc ttcacccgcg acttcgaaga ccataatcag cgagccggta gacactttgt
    10981 cacccacgtt cactttgatc tctttcacgg tgccagcaaa cggagccgga acttccatag
    11041 aagccttgtc gccttctacg gtgatcagcg actgttcagc ttcaacttta tcgccaactt
    11101 tcaccaggat ttcggtcact tcaacttcgt cgctgccgat atccggaaca ttaacgtctt
    11161 ttgccgccgc agccgctggt gctgctgccg gagctgcttc tttcttctct tctgcctgag
    11221 caggtgcagc gtctgctgca ccgtcggcgg aatcgaaaat cataatcagt gcgccggtct
    11281 gggttttatc gccaacagag actttgatct ctttaacgat acccgcctgc ggagacggaa
    11341 cttccataga ggctttgtcg ccttctacgg tgatcagcga ctgttcggct tcaactttgt
    11401 cgcccacttt gaccaggatc tcggtgattt caacttcatc agccccgatg tccggtactt
    11461 tgatttcgat agccattatt cttttacctc ttacgccaga cgcgggttaa ctttatctgc
    11521 atcgatgttg aatttggcga ttgcgtcagc aaccactttc ttatcgattt cgccacgttt
    11581 agccagttcg cccagcgccg caaccacgac ataagaagca tcaacttcga agtggtgacg
    11641 caggttctca cggctgtcgg aacgaccgaa gccatcagta cccagtacgc ggtagtcgtc
    11701 agccggtacg taagtacgga cctgctcagc gaacagtttc atatagtcgg tagatgccac
    11761 tgccggagcg tcattcatca cctgagcgat atacggaacg cgcggagttt ccagcgggtg
    11821 cagcatgttc cagcgttcac aatcctgacc atcacgcgcc agttcggtga aggatgtcac
    11881 gctataaacg tcagaaccta cgccgtaatc tttcgccagg atctcagctg cttcacggac
    11941 gtgacgcagg atagaaccgg agccgagcag ctgaacttta cctttgctac cttcaatggt
    12001 ttcgagtttg tagatacctt tacggatacc ttcctcagca ccttcgggca ttgccggcat
    12061 gtggtagttt tcgttcagcg tggtgatgta gtagtaaacg ttctcttgtt tttcaccgta
    12121 catacgctcc agaccgtcat gcatgatgac agcaacttcg taagcgtaag ccgggtcgta
    12181 agagatacag ttcgggatag tcagcgactg aatgtggctg tgaccatctt cgtgctgcag
    12241 accttcgccg ttcagggtgg tacgaccgga agtaccgccg atcaggaagc cacgcgcttg
    12301 ctggtcacca gccgcccagc acagatcgcc gatacgctgg aagccgaaca tcgagtaata
    12361 gatgtagaac ggaatcatcg gcagattgtt ggtgctgtaa gaggtcgccg ctgccagcca
    12421 ggaacaacct gcgcccagct cgttgatccc ttcctgcaga atctgacctt tctcgtcttc
    12481 tttatagtaa gcaacctgct cgcggtcctg cggggtgtac tgctgaccgt tcgggctgta
    12541 aataccaatc tgacggaaca gaccttccat accgaaagta cgcgcttcgt cggcgatgat
    12601 cggcaccagt cggtctttga tcgacttgtt cttcagcatc acgttcagag cacgaacgaa
    12661 agcgatagtg gtagagatct ctttgctctg ctcttccagc agcgcgccga aatcttgcag
    12721 gctcggcagc tcaagcttct cggtgaagtt cggctgacgg cttggcagat aaccgtgcag
    12781 tttctgacgc tgcgcgtgca gataggtatg ctcttcagaa ccttccggga aggtgatgta
    12841 cggcagtttt tcgatatcgg catcagacac cggcacattg aaacggtcgc ggatgtgacg
    12901 cacgccgtcc atgttcattt tcttaacctg gtgcgcgatg tttttacctt cagccgcgtc
    12961 gcccatgccg taacctttaa tggtatgagc aaggattact gtcgctttgc ctttggtttc
    13021 ctgcgctttc ttgaatgcag cgtagatttt cttcggatcg tgaccgccac ggttcagtgc
    13081 ccagatctgc tcgtcagtcc agtctgcaac cagtgctgcg gtttcaggat atttaccgaa
    13141 gaagtgttca cgaacgtacg caccatcttt cgatttgaag gtctggtagt cgccgtcaac
    13201 ggtttcgttc atcagctgga tcagtttacc gctggtatct ttacgcagca gttcatccca
    13261 acggctaccc cacatcactt tgatcacgtt ccagccagca ccttcgaaga tgccttccag
    13321 ttcgttgatg atcttgccgt taccggtgac cgggccgtca agacgttgca ggttacagtt
    13381 gataacgaag accaggttat ccagtttttc acgggttgcg atggtgatcg cacctttgga
    13441 ttccggttcg tccatttcac cgtcgccgag gaatgcgtaa acggtctgtt tagaggtatc
    13501 tttcaggcca cggtgttcca gatatttcag gaacttagcc tggtaaatag caccaatcgg
    13561 acccagaccc atagatacgg tcgggaactg ccagaattcc ggcatcagtt tcgggtgcgg
    13621 ataggaagag aggccattgc cgtgaacttc ctgacggaag ttatccagct gctcctgagt
    13681 cagacgacct tccaggaaag cacgtgcgta aacgcccggg gagatgtggc cctggaagta
    13741 aaccaggtcg ccgccatcct gctcgttgcg tgcacggaag aagtggttaa agcacacatc
    13801 ataaatggtt gcggaagact ggaaggacgc catgtggccg cccagttcga ggtctttttt
    13861 cgacgcacgc agaaccgtca tgatggcgtt ccagcggata gctgaacgaa tacggcgttc
    13921 cagttccaga ttacccggat actccggttg ttcttcaacg gggatggtgt tgatgtagtt
    13981 gctgatacct gtgcctgcgg ctacgttgac accgcctttg cgggcttcag caagcagttg
    14041 gtcgatcaga tactgagcac gctcaacacc ttcttcacgg atgaccgatt cgatcgcctg
    14101 gagccagtcg cgagtttcga tcggatccac gtcatttggg aaacgttctg acatgggtta
    14161 ttccttatct atctaataac gttgagtttt ctggaacctg tcccattgaa ctctcgccgg
    14221 aaagctcaat aagacaggtt ctacgtttag ttgccgcgct tttatatgcg ctgatttaca
    14281 acatcttctg gataattttt accagaaaaa tcactaattc tttcgttgct ccagacgacg
    14341 cagagaacgc tcacggcggc tctcttcacg gcttctgtcg agcaaaattt cttcgataaa
    14401 ggccagatgg cgatgcgatg cttcgcgcgc ttcttccggc ttaccggcca taatcgcttc
    14461 aaatatgcgg gtgcggtgac tactcaccag cggcagcatc tcgcgacgcg aatagagcaa
    14521 ttcgaagttc tggcggacat tctgggccaa catcggctcc atacacctta gcagatgaag
    14581 cagaaccaca ttgtgggccg cttcggtgac ggcaatctga tactggagta cggcgtttga
    14641 ttccgcgtcc agatcgccag actgctgcgc cagctctata gcgtggtgga gttcacggat
    14701 gcgttccttg tcttcatcgg tactacgcag cgcggcgtaa taagcggcga taccttccag
    14761 ggcgtgtcgt gtttcgagca agtcatactg tgattcagga tggtcggaga gcagctccac
    14821 cagcggatcg ctgaagcttt gccataggct gctctggaca aaagtgccgc caccctggcg
    14881 acgaagcaac aagcccttcg cttcgagacg ttgaatcgcc tcacgcaagg agggacggga
    14941 gacgtcaaac tgttttgcca gttcgcgttc cggtgggagt ttttcgcccg ggcggagagt
    15001 gccttcgagg atcaaaaact ccagttgctg ctcaatcaca tcggagagtt ttggttggcg
    15061 gattttgctg taggccatga gttcctgtct taagccactt gccgaagtca attggtctta
    15121 ccaatttcat gtctgtgacg ctaaagtaac aaagtattca ccttatgtcc atacaggttt
    15181 tgattgaaat catgaaactg tgcacatttt aacaacttga catatataac gtttcaaagt
    15241 tgtaactatg cacaaatgtt aaatttaccc accacgggtt atgattaaca gaattgaaac
    15301 gaaaaaatat ttcttctgaa ccgattcacc aagcaacaaa acgcataaca attcaattat
    15361 gtgaattaat atgtaaaatg aatgttcagg gaaggagaga aaggtagtat ttacctatgt
    15421 tttcttttta cattgcttat tatcactgca atcgccaccg taaccttctc gtagcgaaaa
    15481 tcatcactta accagaacgt gatgattttc aggtgacaga ttctgtttag agttcattct
    15541 gaatctttga acaaagacaa tccttgcgaa cgccgccatt ctttgacgat tttcaggatg
    15601 ttttcaggtt taccatcttc tggggaatta ggatagaaaa tgagatctcc ctttttagga
    15661 tgttcagtaa ttttaatgaa atgatccact aaggtatcga gatatttttc taattttttc
    15721 ccttttaggg atttatcttt gctagttgca tttactatct cctcaagcat ttcaagaaat
    15781 tcaccttctg tgtaaaaata tattttgtat tttaaatcat gcatatacat tatctcctgt
    15841 agtgaatttc atcatgtagt cgaggcgtaa caatccttta attatcaata tcataaattt
    15901 cgccaccaca ttcaatcgca acactatgat atatatggaa ttctttaaca atttccttag
    15961 agccataata ccaaccttca agaggcgctt ttgcgattaa tccttgtttt atacgtgttt
    16021 ggttaccagt tgaaaactgc gctatcaact ccggatactg actcacctcc agccaaaggg
    16081 tatcggtgaa agcgggaaat tgataaaata acctaatgag atttcagtat tttacctgtt
    16141 ttttaagagc acccacatta ttattttatg ttattaacat catcgcatta gccagacgcg
    16201 atgatgttcc atatatagtt ttattatcta ctttatttag agtctttaaa caagggtagc
    16261 ccctgtgaac gccgccattc tttgacaatt ttcaggatgt tttcaggttc atcatctccc
    16321 ttctttgcag gatagtagat aagatcgctc atcaacggat gttgtgtaat tctggtaaga
    16381 tgttcttcta gatcatccca atatttgtca agctcttccc cttttaattg agcgttatcc
    16441 ccggttggat ttgtaaactc gccaagcaac tcaatgaatt cccgctctgt gtaatcttct
    16501 attttatttt taaaatcata catttaacta tctcctgtaa taaatttcat catgcaaatg
    16561 aggtgtgccc tccttaaatt atcaatatcg taaattccgc cactatattc aatcgcaaca
    16621 cgatgatata tgtggaattt tttaacgatt tctttagtgc cataatgcca accttcaagt
    16681 ggtgcttttg cgattaatcc ttgttttata cgtgtttgat taccgcttga aaactgcgct
    16741 atcaactccg gatactgact cacctccagc caaagggtat cggtaaaagc gggaaaatga
    16801 taaaataacc taatgaggct tcagtatttt tacctgtttt ttaagagcaa tcacattatt
    16861 attttatgtc attaatatca tcgcattagc cagatgcgat gatgttccat atatagtttt
    16921 attatctact ttatttagag tctttaaaca agggtaaccc ctgtgaacgc cgccattctt
    16981 tgacaatttt caggatgttt tcaggcttat catctccttg acttttaggg tagaaaatga
    17041 gatcgctccc tgacggatgc tgtgtaattt caataaaatg atctactaat gaatcccagt
    17101 acatttctag ctccttacct tttaaggatt tcccatcttt catagatgat ctcaatccgc
    17161 ctaaaaaatt aagaaattca tcctcggtat aatcctggag cttttcttta aacatattta
    17221 tctcctgtag tgaatctcat cgtgtagtct gggggtaaca attcgtaaat tatcaatatc
    17281 gtatacgctg ccgccatatt caattgcaac acgatgatgt atctggaatt ttttaactat
    17341 ttctttaggg ccataatgcc aaccgtcaat aggtgctttt gcggttaatc cttgttttat
    17401 acgtgtttgg ttaccacttg aaaactgcgc tatcaactcc ggatcctgac tcacctccag
    17461 ccacagcgcc tcgcgaaact catcaaaggt tttaaactcc cgtccgcgca atttatcagc
    17521 aatatgtgcc gggattggtg cgccgttgcc gttattggat accgacatat ccagccagcg
    17581 agttcctggt gaaagcggga cgccgcgccc tgttacaaca ccaggcagct ttcgcggatc
    17641 ctccttcagg taaacataga tgggcttaat accggatccc gccggaaaaa ccagaatata
    17701 atcgttgaat tctttcggat caggaaccgg gaatggcggc gtgctatagg tatcagtgcc
    17761 gtccggaatc ggcgtcacca gaatggtggg ctgctccagc ggcggcctgt cgttaccggt
    17821 atggcccgga acattcccat gttccggatt ttccggcgtc caggtaatca gggggccatc
    17881 gacatcgtga gcgggggtaa attcataacg ctgcgtctgc tcgttccact gcatgttcgc
    17941 cacgcggacg ttttcatacg gcgtaccttc acccgtatga acaccataca cctgaggctt
    18001 gccgtgaatg tcagttcccc agaaaaaacg tacccgggtg gtggcggtac tgcctgctag
    18061 tgctttctgc tgcagttctt cctcatacca gaactcatca cgtccgggca cgcgagcact
    18121 gccctcgccc gcagagggaa tatacaaagc agatgccaac gcaccgatgt aagagccgcg
    18181 ggttgcactg agtatgccac tgagcgacca ttttccccat atttgagccg ccaatacgct
    18241 acccggaaac agtagcatgg tttgagcttc tgcgggttgc ccgacatcgt tagcctcggg
    18301 atggtggtat ccacagcagt catcaaccac tggcagagca aaaatcgcta cttgcccgaa
    18361 gttttcagcg ggttcttctg ccgttccggc atctgtacat cccttctcct tcagacagga
    18421 tttggcatag accggtttaa ccgctggtgg agagagtgga aggttaacaa ctggtagagg
    18481 ctcagggtta agattactgg caggtgggtt tttaggtgga ataaataagc tattatggcc
    18541 agcaggaaag gccaaccagt caaattccgt attggctatt agatgtttac ataatatgta
    18601 gtcataatcg acagttacac attcggtagg aagattattg tcagaaatgt ttacatgaat
    18661 agcagttaat gaagcatttc ttagctgaat ataataatac cgttcccagc gcccgctttt
    18721 atttatacga tacaaatcaa tttcaataaa taaccgctca ttctgattga tggcattaga
    18781 cagtagcggt gagcttttat caatgagttt gcaaaattgt agcccctgca gattaacgcc
    18841 tttaccagta ctggtaatgg tgttggtgag tgcaaaggca aaaatttcat cttcatggcc
    18901 aacctgccag cgattaccaa ccgacgcata cgtcccacag cctgaagaaa tatcgccctg
    18961 cttctcaccg attattctca ggtaaacaat atcgcccact ttatttcatt ccagtttatc
    19021 cttaacttat ttgcattaaa tagaaaactt atttctcgat caattataaa cgcttttaat
    19081 gaaaatttat ttcttttact taacgttttc actcatctcg caatattata tttaaatact
    19141 tacgttacaa gcccaccgca ttattaacat ctatttattt ttatatgacc ttttgtaaga
    19201 ttattgtgct ggtggctaag gcatggtgtt cagcaggcag acaaatccgg catatagcta
    19261 gttaaaacag tatgttatca gacttaaagt atctattaaa tgtgatgaaa aacttacaat
    19321 tgcttttttt ttatccagaa aatcaacaca tctacataaa gcaggtgcat tctcgcgcac
    19381 ataccattat cttgtgatag ttaaaagtag cattatcata attcagttac atgaaacgta
    19441 aagaaatcaa tacaataaac ggaattgcaa acttacacac gcatcactgc gtagatcaaa
    19501 aaaacaatca ccgcacgagg tttcatgatg gaaggtcaac agcacggcga gcagctaaag
    19561 cgcggcctta aaaaccgcca tattcagctt atcgcgctgg gtggcgcgat agggaccggg
    19621 ttattcctgg gtagcgcctc cgtaatacag tccgcagggc cagggattat cctgggttac
    19681 gccattgctg gttttatcgc ctttctgatc atgcgtcagc tgggtgaaat ggtggtcgaa
    19741 gaacctgtcg caggctcctt tagccacttt gcttataaat actggggcag ttttgccggt
    19801 ttcgcctctg gctggaacta ctgggtactg tacgttttag ttgccatggc agagctgact
    19861 gccgtgggta aatacattca gttctggtat ccggaaatcc caacctgggt ttctgccgcc
    19921 gtgttctttg tggtgatcaa cgccatcaac ctgacaaacg taaaagtgtt tggcgagatg
    19981 gagttctggt ttgccattat caaagttatc gcggtggtag cgatgatcat cttcggcggc
    20041 tggctgctat tcagtggcaa cggcggcccg caggcgaccg ttagcaacct gtgggatcag
    20101 ggtggtttcc tgccgcacgg cttcaccggg ctggtgatga tgatggcgat tatcatgttc
    20161 tcgttcggtg gtctggaact ggtggggatc accgcagcag aagctgataa cccggagcaa
    20221 agtattccaa aagcgactaa ccaggttatc taccgcatcc tgattttcta cattggttcg
    20281 ttagccgttc tgctctcact gatgccgtgg acacgcgtta ccgctgacac cagcccgttt
    20341 gtgctgatct tccacgagtt aggcgatacc tttgtggcaa atgcgctgaa catcgtggta
    20401 ctgactgcgg cgctctccgt gtacaacagc tgcgtatatt gcaacagccg tatgctgttt
    20461 ggtctggcac aacagggcaa cgcgccaaaa gcgctggcgt ctgtcgacaa acgcggtgta
    20521 ccggttaata ctattctggt gtctgcgctg gttacggcat tgtgcgtgct gattaactac
    20581 cttgcaccgg aatccgcatt cggcctgtta atggcgctgg tggtatctgc actggtcatc
    20641 aactgggcga tgattagcct ggcgcatatg aaattccgtc gcgccaagca ggaacaaggc
    20701 gtggtaactc gcttccctgc tctgctttat ccgctgggta actggatctg cctgctgttt
    20761 atggcggcgg tattggtgat tatgctgatg accccaggaa tggcgatttc ggtatacctg
    20821 atcccggtat ggctgatcgt gttaggtatc ggctatctgt ttaaagagaa aaccgccaaa
    20881 gccgtaaaag cgcattaatc tctctacgcc ctcacccgtg cagggtgagg gcaataatct
    20941 ttacaccaac gccccgtaaa tcgtcagtag cgcaattacc accacgacca cgaacgaggt
    21001 tttcttcgcc atggaaaccg ctgccttcgg cgtctccacc ttatcgacat gcggttcacg
    21061 cgccagagag aactgtgcca gacgcgttaa tacctgatat tgcgaagtat ggaaatcacc
    21121 cagcgaagca aaccaggccg gtaacgcttt ctccccatga ccgatcaagg catataccac
    21181 acccgcaaga cgaaccggca cccaatccag tacatgaagc acgccatcaa tgccggactg
    21241 taaacgatga tgcggcgtct ggtaacgcgc cagccagtat tgccatgcac gcaaaaacgc
    21301 ataccccatc agcgtaacgg gtccccaggt tccccccaca atcagccaga acagcggtgc
    21361 aagataaaaa cgaaagttaa tccacagcaa tgcattttgc agctcacgca aatactcacg
    21421 ttcatcgcag cctgccggga cgccgtgaat catggtgagt tcgccagcca tcgtggcacg
    21481 ggcatggcta tcattacgtg aagcagctgt cagataagca tgataatgaa gacgaacttt
    21541 acctgcgcca atacacagca aaccaatcag cagccacacc agcagcgtgg gaacgttgaa
    21601 taatactccc tgcaatgtgc gtaacagtaa aaaagtcacg cccatcgcaa taatggtcat
    21661 gcctaatgtg cgcccgagag aaaaatgttt cacccgccga aagaacgctt caagacgatg
    21721 atcaagctgc cagtgctcgc ccaacttaaa caggcgctcg aaaattaaca ccagtaaggt
    21781 tgtaaatagc gtcatgttgt ctccttgctg accagtgcac gaaaccgtgc ccaatcaaat
    21841 gcaggaccgg gatcggtttt ccgatccggc gcaatatcac aatggcccgt catgttatta
    21901 gcgatatctg gatagcgatc aatcagtgcc cgcgtaaccg ccgcaagctg ttgatactgc
    21961 gcatcggtat acgccagcgt atcggtgcct tccagctcaa tcccaataga aaaatcattg
    22021 cagcgttcgc gcccctgata ctgagagact ccggcatgcc aggcacgttt atcgaaagga
    22081 acatactgga ctatttcacc atcgcggcga atcaaacagt gagcggagac gcgcaaatgg
    22141 gcgatctcag caaagaaagg atgtgcctgc ggatcaatag ttccagtgaa taatgcgtcg
    22201 atccacggac cgccaaactc gcctggcggc aggctaatat tgtgcaccac cagcagggtg
    22261 ggtgtttcgt catccgggcg gcaatcgtaa tgtggtgagg gaacgcggcg cgcgccagcc
    22321 agccacccct gttctaacaa catgcaggat ctccttatat gtggtgctaa tacccggttc
    22381 agagtagcat gtttctacct tatgattcgt tagctatctg gagttttaac atgccgcctc
    22441 gccgctataa ccctgacacc cgacgtgacg agctgctgga acgcattaat ctcgatatcc
    22501 ccggcgcggt ggcccaggcg ctgcgggaag atttaggcgg aacagtcgat gccaacaatg
    22561 acattacggc aaaactttta ccggaaaact ctcgctctca tgccacagtg atcacccgcg
    22621 agaatggcgt attttgcggc aaacgctggg ttgaagaggt gtttattcaa ctggcaggcg
    22681 acgatgtcac cataatctgg catgtggatg acggcgatgt catcaacgcc aatcaaccct
    22741 tgttcgaact tgaaggccca tctcgcgtgc tgttaacggg cgaacgcact gcgcttaatt
    22801 ttgtgcaaac cctttcagga gttgccagta aggtacgcca ctatgtcgaa ttactggaag
    22861 gcaccaacac gcagctgttg gatacgcgca aaaccttacc cggcctgcgt tcagctctga
    22921 aatacgcggt actttgcggc ggcggagcga atcaccgcct ggggctttct gatgccttcc
    22981 tgatcaaaga aaatcatatt attgcctccg gctcagtgcg ccaggcggtc gaaaaagcgt
    23041 cctggttgca cccggatgcg ccagtagaag tcgaagtaga gaatctggaa gaacttgatg
    23101 aagccctgaa agcaggtgcc gatatcatca tgctggataa cttcgaaaca gaacagatgc
    23161 gcgaagccgt caaacgcacc aacggcaagg cgctactgga agtgtctggc aacgtcactg
    23221 acaaaacact gcgcgaattt gccgaaacgg gcgtggactt tatctccgtc ggtgcgctaa
    23281 ctaaacacgt acaggcactc gacctatcaa tgcgtttttg ctgattgctg acagatttat
    23341 ccgctccatc gcgggcggat aatctcccct ttcccaactt tcttcgtaac gcctcgcaaa
    23401 tttcttgtct ttcgctgcaa cgtaataaaa aactccggaa gctgctttcc cgtttcgttt
    23461 tttgcccctc gcttgtgctg atggcgtttg ccaaagtagc gccaaccaaa tcaaggagcg
    23521 aaacaaatgg acaagcaacg cggttttaca cttatcgaac tgatggtggt tattggcatc
    23581 attgccattt taagcgccat tggtattccc gcttatcaaa actacctgcg caaagccgca
    23641 ctcaccgaca tgctacaaac ctttgtgcct taccgtaccg ccgtagagtt gtgcgcgctg
    23701 gaacatggtg gattaaatac ctgcgacggt ggcagcaatg gtattccttc gcctaccacc
    23761 acccgctatg tttcagccat gagtgtggca aagggcgtgg tgtcgctgac cgggcaagaa
    23821 agtctcaatg ggctaagcgt cgtcatgacg ccgggttggg ataacgcaaa cggcgtcacc
    23881 ggctggacgc gcaactgcaa tattcaaagt gacagcgcat tgcagcaagc ctgcgaagat
    23941 gtcttccgct ttgatgacgc caactaagga gcggcaatga atattccaca gctcaccgcc
    24001 ctgtgtctgc gttatcaggg agtcttgctg gatgccagcg aagaggtggt tcatgttgcg
    24061 gtagtcgatg caccttcgca tgagctactg gacgcattgc atttcgctac caccaaacgt
    24121 attgagatca cctgctggac gcgccaacaa atggaaggtc acgccagtcg cacacaacag
    24181 acattgcccg tagctgttca ggagaagcat cagcccaaag cagagttgct gactagaacg
    24241 ttacagtctg cgctggaaca acgcgcgtct gatattcata tcgaaccagc ggacaatgcc
    24301 taccgcatcc gcttgcgtat cgacggcgta ttgcatcctt taccggtcgt ttcaccggat
    24361 gccggagtcg cattaaccgc cagattaaaa gtgctgggaa atctggatat tgcggaacat
    24421 cgcctgccgc aggacgggca attcactgtc gaactggcag gaaacgccgt ctcatttcgt
    24481 attgcgacct taccatgtcg gggtggtgaa aaggtggtat taaggttgtt acagcaggtg
    24541 agccaggcac tggatgttaa cacgctggga atgcagccgt tacaactggc ggactttgct
    24601 catgcattgc aacaaccaca gggattggtg ctggtaactg gccctacagg cagcggcaaa
    24661 acggtcacgc tttatagtgc cctgcaaacg ctgaataccg ctgacattaa tatttgtagc
    24721 gtcgaagatc cggttgagat ccccatagcc ggactaaacc agacgcaaat ccattcgcgt
    24781 gccggactca cctttcaggg cgttttgcgt gcgttattgc gccaggatcc tgacgtcatc
    24841 atgatcggag agatccgcga tggcgaaacg gcagaaattg ccattaaagc cgcgcaaacc
    24901 ggtcacctgg tgttgtctac cctacacact aattccacct gcgaaacgct ggtacgttta
    24961 cagcaaatgg gagtcgcccg ctggatgcta tcatcggcgc ttacgctggt aatagcccag
    25021 cgtctggtac gcaaactttg cccgcattgt cgccggcagc aaggggagcc catccatatt
    25081 ccagacaatg tatggccgtc gccgctgccc cactggcagg cacccggttg tgtacattgc
    25141 taccacggtt tttatggtcg tacggcctta tttgaagttc tacccataac accgatcata
    25201 cgccagctta tttccgctaa taccgacgtt gaatcgctgg aaacacacgc ccgacaggcg
    25261 ggtatgcgta cgctttttga aaacggctgc ctggccgtgg agcaaggctt aaccaccttt
    25321 gaagagttaa tccgcgtatt ggggatgccg catggcgagt aagcaactct ggcgctggca
    25381 tggcataacc ggcgacggca atgcgcaaga tgggatgcta tgggctgaga gccgtgcttt
    25441 gctgctcata gcactacagc aacagatggt taccccactt agcctgaagc gaatcgccat
    25501 caattctgcg cagtggcgag gagataaaag cgcggaagtc attcatcaac tggcgacgct
    25561 actcaaagcc gggttaacgc tttctgaagg gctggcactg ctcgcggaac agcatcccag
    25621 taagcaatgg caagcgttgc tgcaatcgct ggcgtacgat ctcgaacagg gcattgcttt
    25681 ttccaatgcc ttattaccct ggtcagaggt atttccgccg ctctatcagg cgatgatccg
    25741 cacgggtgaa ctgaccggta agctggatga atgctgcttt gaactggcgc gtcagcaaaa
    25801 agcccagcgt cagttgaccg acaaagtgaa atcagcgtta cgttatccca ttatcatttt
    25861 agcgatggca atcatggtgg ttgtggcaat gctgcatttt gttctaccgg agtttgccgc
    25921 tatctataag atctttaaca ctccgctacc ggcgctaacg caggggatca tgacgctggc
    25981 aagttttagt ggcgaatggg gttggttgct ggtgctattc ggctttctgc tggcgatagc
    26041 caataagctg ctgatgcgcc acccaaccag gcttatcgca cggcagaaat tgctgttacg
    26101 catcccgatt atgggttcac tgatgcgggg acaaaaactc acgcagatct ttacgattct
    26161 gacgctgaca caaagtgcag gcatttcttt tttacagggc gttgagagcg tcagagaaac
    26221 aatgcgctgc ccgtactggg tgcaacttct gacacaaatc cagcacgata tcagtaacgg
    26281 tcaccccatc tggctggcgc taaaaaatgc cggtgagttt agcccgctct gtttgcaatt
    26341 agtgagaaca ggagaggcat ccggctcgct ggatctcatg ttagacaacc tcgcccatca
    26401 tcatcgggat aacacaatgg cgctggcgga taacctcgca gccttactgg aaccggcgtt
    26461 gctgatcata acgggaggaa ttatcggtac gctggtggtg gcaatgtatc tgccaatttt
    26521 ccatttaggc gatgcgatga gtggaatggg ataacgctgg cgtgttgctc cacgccagcg
    26581 ttggtatatt acaggttgtt gaagatgcgg ttttcttgtt cctgcacacg gataaatgtg
    26641 gtgcgcttgg tcagctcttt caggcgtgaa gccccaacgt atgtacaagc tgaacgcagg
    26701 ccgcccaaaa tatcacgcgc ggtattttca accgggcctc gcagcggcag cttaacggtt
    26761 ttaccttctg ctgcgcgata ttccgcaacg cagccaacgt gacgtttcat cgcagactct
    26821 gagctcatgc cgtagaacag cataaatttc tcgccgttct cctcaacgat gcgaccgccg
    26881 ctctcttcgt ggcccgccag catgccgcca agcatgacga aatcggcacc gccgccaaag
    26941 gctttcgcga catcgcccgg cgtggtgcag ccaccgtcgc tgacgatcat tccgcccaga
    27001 ccgtgcgcag catcggcaca ttcgattacc gcagaaagtt gcggataacc gacgcctgtt
    27061 ttgacgcgag ttgtacaaac agaacctggg ccaatgccaa ctttaacgat atcggcaccg
    27121 gagaggataa gctcctcaca catttcacca gtcactacgt tgccagcaca aatggttttg
    27181 gtcggccacg cttcacgcgc tttcgcaacg aactgcacga agtgttcgga ataaccattc
    27241 gccacgtcaa tacaaacgaa gtttaatgcc gggttcaggt cgagaatctg tttagttttt
    27301 tcgaaatccg catcagacgt gccggtagaa accatcacat gtttcagcac atcagcggaa
    27361 gaattgttga taaacgcttg ccactcttcg acagaatagt gtttatgcac agcagtcaaa
    27421 atatcaaaag aagccagcgc agaggccata gaaaatgtgc ctacggtgtc catatttgcg
    27481 gcgataatcg gcacgccgga ccagctctga cctgaatgtt tgaaggtgaa ttgacgttcc
    27541 agtgcaacat cggaacggct tttaagagtg gagcgtttag ggcggatgag aacgtcttta
    27601 aaacctaact tcagatcttc ttcaatacgc atgtgcggat tcctggggtt aatggctata
    27661 agggtaagag cgcaactcca gtgacgctat catacgcact aatcaatgcg gcgcaagact
    27721 gcgaaattgt ctttttttac gctacaatcc cataaattta cctggtaaat agtgagcgaa
    27781 cgtttcatca atttcgcttt cgtgcgctca tttttaacgt tttgataatc atgatgaatt
    27841 cccgggaata taaattatga ggtatatagt tgccttaacg ggaggcattg gcagtggcaa
    27901 gagtaccgtt gccaatgcgt ttgctgatct cggaattaac gtcattgatg ccgatattat
    27961 tgcgcgtcag gtggttgaac caggtgcacc tgcgctacat gccattgctg atcactttgg
    28021 cgctaacatg attgctgctg atggaacatt gcagcgccgg gccttgcgcg agcggatctt
    28081 cgccaacccg gaagagaaaa actggcttaa cgccctgctg catccgctga ttcagcaaga
    28141 gacgcaacac cagatccagc aagcaacttc cccctatgta ctgtgggttg tgccattgct
    28201 ggtagaaaac tcactgtata aaaaagcgaa tcgagtgctg gtggtggatg tcagcccaga
    28261 aacgcaactt aagcgcacca tgcagcgcga cgatgtaact cgcgagcatg tcgaacaaat
    28321 ccttgctgct caggcaacgc gcgaagctcg ccttgccgtg gcagatgacg tcattgataa
    28381 taacggcgca ccggatgcaa tcgcatcgga tgttgcccgc ctgcacgcac actatttgca
    28441 gcttgcgtcg cagtttgtct cacaggaaaa accgtaatgc agacccaggt cctttttgaa
    28501 catccactaa atgaaaaaat gcgtacatgg ctgcgcattg agtttttgat tcagcaactc
    28561 accgttaatc tacccatcgt tgaccacgct ggcgcgctgc atttcttccg taatgtcagt
    28621 gaattactgg atgttttcga gcgcggcgaa gtccgcactg agctgttgaa agaacttgac
    28681 cggcagcaac gtaaactcca gacctggatt ggcgtgcctg gcgtggacca gagccgtatt
    28741 gaagcattaa ttcagcagtt aaaagcggcg gggagcgtat taatttccgc gccgcgtatc
    28801 gggcaatttc tgcgtgaaga tcgtttgatt gctctggtgc gtcagcgact gagcatccca
    28861 ggcggctgtt gtagctttga tttacctaca ttgcacattt ggctgcatct accccaggcg
    28921 cagcgcgaca gccaggtaga aacctggatt gccagcctga acccgctcac ccaggcactt
    28981 accatggtgc tggatttaat tcgccagtcg gcccccttcc gtaaacaaac cagcctgaat
    29041 ggtttttatc aggataacgg tggcgatgcc gacttgctgc gcctgaatct gtcactcgat
    29101 tcacagcttt atccgcaaat ttccggtcat aagagccgtt ttgccattcg ttttatgccg
    29161 ctggacagtg aaaacggaca ggtaccagaa cgtctggatt tcgaactggc ctgttgctaa
    29221 ggagtaaaaa tgtcagaaac tattacggtg aattgcccaa cctgcgggaa aacggtggtg
    29281 tggggtgaaa tcagcccgtt tcggccattt tgttccaaac gttgccagct gatcgacctc
    29341 ggagaatggg ctgctgaaga aaaacgaatc cccagcagtg gcgatctttc cgaaagcgat
    29401 gactggagcg aagagccaaa gcagtgacat tttgtctgat gcgacgctgg cctgataaga
    29461 cgcgggcagc gtcgcatcag gcatcgtgtg caaatgtcgg atgcggcata aacacctatc
    29521 cgacgtacac gcgcttaagc ttcgcaatta ccggttcatt ggctggcgga aactcatcgg
    29581 cattaagacc gaccagcgac atccacttac cgggttgccc ttctttaccc cacggctccc
    29641 cttcccagct ttcgaccagc cagaaccaca gagttatatg cctgtccggg aattcatatt
    29701 ccagtttttc aaatagcgaa aaatgttgtg gggtaatccc gacctcttcc tgaagttcac
    29761 gcaccaccgc ctgttccggc gtttcgctca tttcaatttt accgccggga aactccagtt
    29821 tattcgccat gtgcgcatct gctgcgcgac gggttataaa gatttcattg ttctcgttgc
    29881 gaataatacc taccgcaatt tgcagctttt tcattgtctt aaacctataa aaaaggcgca
    29941 gaatcctgcg ccttttactt caactgttag cttttattgc aggcgaccat ggcactgctt
    30001 gtatttttta ccagaaccgc acgggcaagg atcgttacgt cctactttgc gctcaccggt
    30061 ttgcgccgcc agtgcagctg cagctgcaga gtcgtcatcc tgatggctaa gctgctgcat
    30121 ttgcgctaaa cgctcggctt ccatacgacg ctgttgttcc agctcctcaa cctcttcagg
    30181 catacgtacc tgaactttgc tcagcgtgct gataacttca tatttcaacg actccagcat
    30241 cgctgcaaac atggagaacg attcacgttt gtattcctgc ttcggatctt tctgtgcata
    30301 gccacgcagg tggataccct gacgcagata gtccatcgcc gccaggtgct ctttccacag
    30361 agagtcgaga gtttgcagca tgacgccttt ttcgaagtga cgcatcatct cagcaccaac
    30421 cacttcttct ttacgctgat acacttcgat ggactgcgcc agaatgcgct cacgcagcgt
    30481 ctcttcatgc agttctggtt ctttatccag ccactcggca attggcaaat cgaggtcgaa
    30541 atcgttcttc agacgttcct gcagtcccgg aatatcccac atttcttcca gcgactgtgg
    30601 cggaatgtag gcatcaatgg tcgctttgaa cacatcttca cgaatgctgt tgatggtttc
    30661 gctcacatcg ctgacatcca gcagttcgtt acgctgggag taaatggcgc gacgctgatc
    30721 gttagccacg tcatcatatt ccagcagttg cttacgaatg tcgaagttac gactttcaac
    30781 tttacgctgg gcgttggcaa tcgctttagt cacccacggg tgttcaatgg cttcgcctgg
    30841 cttcataccc agtttacgca tcatgccgga tactcggtcg gaagcaaaaa tacgcatcag
    30901 cgcatcttcc atcgacaggt agaaacggga agaaccagca tccccctgac gaccagaacg
    30961 accgcgcaac tggttatcga tacgacggga ttcgtgacgt tcagtaccga tgatatgcag
    31021 gccacctgct tccagtaccg catcgtgacg aacctgccag tcggccttaa ttttttcaat
    31081 ttgctctgcg gtcggatttt ccagcgcggc aacttctgcc tgccagctac caccgagcac
    31141 aatatctgta ccacgacccg ccatgttggt cgcgatagtc accgcagccg gataacctgc
    31201 ctgagcaaca atcgccgctt cgttggcatg gaatttggcg ttcaggacgt tgtgcttaat
    31261 accggctttg gtcagttcgt ttgacaccag ctcagatttt tcgatggaga tagtacccac
    31321 cagcaccggc tggcctttcg cagtacgttc tttgatatct tcaatgatcg cctgaatttt
    31381 ttccgcttca gtcatgtaga ccaggtccgg cagatcttta cgaatcattg gacggttggt
    31441 cggaacaacg acggtatcca gcttatagat ggagctgaat tcgaaagctt cggtatcagc
    31501 agtaccggtc atccccgcca gtttttcata cagacggaag tagttctgga aggtgatcga
    31561 cgccagcgtc tggttttcgt tctggatctg tacaccttct ttcgcttcca ctgcctgatg
    31621 caggccatcg gaccaacgac gtccctgcat ggtacgaccg gtgtgttcgt caacgatgat
    31681 aacttcacca tctttaacga tgtagtcgac gtctcgggta aacagcgcat gagcgcgcag
    31741 cgccgccgtt acgtggtgca tcagcatgat gtttgccgga gagtacagag actccccttc
    31801 atccatgatg ccttctttca ccagcagttc ttcaatcagc accagaccac gttcggtcag
    31861 gttcacctgg cgagattttt cgtccaccga gaagtggcct tcgccctgga aggtttcgga
    31921 gtcttctttt tcctgacgga tcaggtgtgg aataatttta ttcacgcgtt tatacatttc
    31981 cgagctgtct tctgccgggc cggaaatgat cagcggtgta cgcgcttcat cgatcaggat
    32041 ggagtccact tcgtccacca gcgcatagtg cagtttacgc tgtacacgtt cttcaggact
    32101 gaacgccatg ttgtcgcgca ggtagtcaaa gccgtattcg ttgttcgtac cgtaagtgat
    32161 gtcagctgcg taagcttcac gctttgccgg tgctggcatg cccggcaggt tgataccaac
    32221 agtcaggcca aggaattcaa acagcggacg gttgttttcg gcgtcacgtt gcgccaggta
    32281 gtcgttgacg gtaactacat gcacgccttt accggttagt gcgttcaggt aagcaggcag
    32341 cgttgcggtc agggttttac cttcaccggt acgcatttcg gcgatgcagc gttcgttaag
    32401 aaccataccg ccgagtaact gaacgtcgaa gtgacgcata ccaaaaacac gcttactggc
    32461 ttcacgcacc acagcgaagg cttccgggat caagttttcc agcacttcgc ctttttccag
    32521 acgcgcgcgg aactctgcgg ttttcccttt cagttcttca tcggagagtt tttccatctc
    32581 cggttccatg gcattgatga tgttgaccac tttgcgcatc cggcgcaggg tgcgatcgtt
    32641 acgactaccg aaaactttag ttaataattt gattagcata ataaaatctc aaacgccccg
    32701 cgttgcggag tcaataaaat gaagtaaagg tttattgttg ttagctgagg cgttgagggc
    32761 cagcacggat gccttgcgcc tggtttatcc agacgggcgt gctgaatttt gcttgcgggg
    32821 taaaatgcgc ataatcaatg cgataaccct ttccagacgg cgtgccttcc tgggtcaaca
    32881 gcgcgctgag cgtatccagt aatgcaagat gttgcgcctg aagaggcaaa gattcttcag
    32941 caacgggcag cgtttgcggt gccattgcga aggaaagatg gcggattacc gtgcgaatgg
    33001 catgttgatg ccagtaatca acggaatagt tcgaattcgg gcggcgcgtg ttcgcttcca
    33061 gcaaggccaa ttgaccaaag ttaactttgg ctgaaggctc gtggttgcgg gttgtcgctt
    33121 ttgcgggcgc gtttggttcg gcggcattgc tgagtgcagg caaacctaaa ctcgccgcga
    33181 ccatccctaa taagagatgc ggccagaagt agcgtttacc aaactgtcgc cagcgcgtca
    33241 gtattccact cacgttattg ccatcccgat cgtattcaaa cgtatttagc gcacaaatct
    33301 tatcattaaa gcctgaagtc cacagcacga atgaagataa gttgtgcgta aaagtgctta
    33361 ataaaacagc gttcgcaccg ttttttatag ctcttataaa attcaaaaaa aaacgactgg
    33421 ttcacctggc cggagagagt gccagatttg ccagtcgaat tttatacgac agtataaatg
    33481 tcgttatgcc agtacggctg aaggcgcttt gaaggccaac ggcagttctg cgtcgtcctg
    33541 gaaggtcaca tattcccagg cttcctgttt tgcaaggaca gcctgcagca gtttgttatt
    33601 cagtgcatga ccagatttat aagcggtaaa tgcaccaata atattgtgac cacacatgaa
    33661 caagtcaccg atcgcat
//
LOCUS       DAMZFT010000044        33482 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.044, whole genome shotgun sequence.
ACCESSION   DAMZFT010000044 DAMZFT010000000
VERSION     DAMZFT010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 33482)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33482)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33482
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.044"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(<1..916)
                     /locus_tag="PN977_002155"
     CDS             complement(<1..916)
                     /locus_tag="PN977_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HDI6042184.1"
                     /translation="MKNSKAILQVPGTMKIISAEMPVPKEDEVLIKVEYVGICGSDVH
                     GFESGPFIPPKDPNQEIGLGHECAGTVVAVGSRVRKFKPGDRVNIEPGVPCGHCRYCL
                     EGKYNICPDVDFMATQPNYRGALTHYLCHPESFTYKLPDNMDTMEGALVEPAAVGMHA
                     AMLADVKPGKKIIILGAGCIGLMTLQACKCLGATDIAVVDVLEKRLTMAEQLGATVVI
                     NGAKEDTIARCQQFTEEMGADIVFETAGSAVTVKQAPYLVMRGGKIMIVGTVPGDSAI
                     NFLKINREVTIQTVFRYAYRYPVTIEAIS"
     gene            complement(933..2312)
                     /locus_tag="PN977_002156"
     CDS             complement(933..2312)
                     /locus_tag="PN977_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDI6042185.1"
                     /translation="MEQITKPHCGARLDRLPDCRWHSSMFAIVAFGLLVCWSNAVGGL
                     ILAQLKALGWTDNSTTATFSAITTAGMFLGALVGGIIGDKTGRRNAFILYEAIHIASM
                     VVGAFSPNMDFLIACRFVMGVGLGALLVTLFAGFTEYMPGRNRGTWSSRVSFIGNWSY
                     PLCSLIAMGLTPLISAEWNWRVQLLIPAILSLIATALAWRYFPESPRWLESRGRYQEA
                     EKVMRSIEEGVIRQTGKPLPPVVIADDGKAPQAVPYSALLTGVLLKRVILGSCVLIAM
                     NVVQYTLINWLPTIFMTQGINLKDSIVLNTMSMFGAPFGIFIAMLVMDKIPRKTMGVG
                     LLILIAVLGYIYSLQTSMLLITLIGFFLITFVYMYVCYASAVYVPEIWPTEAKLRGSG
                     LANAVGRISGIAAPYAVAVLLSSYGVTGVFILLGAVSIIVAIAIATIGIETKGVSVES
                     LSIDAVVNK"
     gene            complement(2339..3415)
                     /locus_tag="PN977_002157"
     CDS             complement(2339..3415)
                     /locus_tag="PN977_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HDI6042186.1"
                     /translation="MKALARFGKAFGGYKMIDVPQPICGPEDVVIEIKAAAICGADMK
                     HYNVDSGSDEFNSIRGHEFAGCIAQVGEKVKDWKVGQRVVSDNSGHVCGVCPACEQGD
                     FLCCTEKVNLGLDNNTWGGGFSKYCLVPGEILKIHRHALWEIPDGVDYEDAAVLDPIC
                     NAYKSIAQQSKFLPGQDVVVIGTGPLGLFSVQMARIMGAVNIVVVGLQEDVAVRFPVA
                     KELGATAVVNGSTEDVVARCQQICGKDNLGLVIECSGANIALKQAIEMLRPNGEVVRV
                     GMGFKPLDFSINDITAWNKSIIGHMAYDSTSWRNAIRLLASGAIKVKPMITHRIGLSQ
                     WREGFDAMVDKTAIKVIMTYDFDE"
     gene            complement(3785..4057)
                     /locus_tag="PN977_002158"
     CDS             complement(3785..4057)
                     /locus_tag="PN977_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaC family protein"
                     /protein_id="HDI6042187.1"
                     /translation="MNLDDIINSMTPEVYQRLSTAVELGKWPDGVALTEEQKENCLQL
                     VMLWQARHNTEAQHMTIDTNGQMVMKSKQQLKEDFGISAKPIAMFK"
     gene            complement(4099..4512)
                     /gene="msrB"
                     /locus_tag="PN977_002159"
     CDS             complement(4099..4512)
                     /gene="msrB"
                     /locus_tag="PN977_002159"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HDI6042188.1"
                     /translation="MANKPSAEELKKNLSEMQFYVTQNHGTEPPFTGRLLHNKRDGVY
                     HCLICDAPLFHSQTKYDSGCGWPSFYEPVSEESIRYIKDLSHGMQRIEIRCGNCDAHL
                     GHVFPDGPQPTGERYCVNSASLRFTDGENGEEING"
     gene            4854..5849
                     /gene="gapA"
                     /locus_tag="PN977_002160"
     CDS             4854..5849
                     /gene="gapA"
                     /locus_tag="PN977_002160"
                     /EC_number="1.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006322611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HDI6042189.1"
                     /translation="MTIKVGINGFGRIGRIVFRAAQKRSDIEIVAINDLLDADYMAYM
                     LKYDSTHGRFDGTVEVKDGHLIVNGKKIRVTAERDPANLKWDEVGVDVVAEATGLFLT
                     DETARKHITAGAKKVVMTGPSKDNTPMFVKGANFDKYAGQDIVSNASCTTNCLAPLAK
                     VINDNFGIIEGLMTTVHATTATQKTVDGPSHKDWRGGRGASQNIIPSSTGAAKAVGKV
                     LPELNGKLTGMAFRVPTPNVSVVDLTVRLEKAATYEQIKAAVKAAAEGEMKGVLGYTE
                     DDVVSTDFNGEVCTSVFDAKAGIALNDNFVKLVSWYDNETGYSNKVLDLIAHISK"
     gene            5933..6817
                     /locus_tag="PN977_002161"
     CDS             5933..6817
                     /locus_tag="PN977_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-hexose-6-phosphate mutarotase"
                     /protein_id="HDI6042190.1"
                     /translation="MIKKIFALPVIEQISPVLSRRKLDELDLIVVDHPQVKASFALQG
                     AHLLSWKPAGEEEVLWLSNNTPFKNGVAIRGGVPVCWPWFGPAAQQGLPAHGFARNLP
                     WTLKSHREDADGVALTFELTQSEETKKFWLHDFTLLAHFRVGKACEIDLEAHGEFETT
                     SALHTYFNVGDITKVSVSGLGDRFIDKVNDAKEDVLTDGIQTFPDRTDRVYLNPQDCS
                     VINDEALNRIIAVGHQHHLNVVGWNPGPALSVSMGDMPDDGYKTFVCVETAYASETQK
                     VTKEKPAHLAQSIRVAKR"
     gene            complement(6868..7722)
                     /gene="yeaE"
                     /locus_tag="PN977_002162"
     CDS             complement(6868..7722)
                     /gene="yeaE"
                     /locus_tag="PN977_002162"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal reductase YeaE"
                     /protein_id="HDI6042191.1"
                     /translation="MQQKMIQFSGDVSLPAIGQGTWYMGEDASQRKTEVAALRAGIEL
                     GLTLIDTAEMYADGGAEMVVGEALTGLRENVFLVSKVYPWNAGGQKAINACEASLRRL
                     NTDYLDLYLLHWSGSFAFEETVAAMEKLIAQGKIRRWGVSNLDYADMQELWQLPGGNQ
                     CATNQVLYHLGSRGIEYDLLPWCQQQQMPVMAYSPLAQAGRLRNGLLKNAVVNEIAHA
                     HNISAAQVLLAWVISHQGVMAIPKAATIAHVQQNAAVLEVELSSAELAMLDKAYPAPK
                     GKTALDMV"
     gene            complement(7812..8558)
                     /gene="mipA"
                     /locus_tag="PN977_002163"
     CDS             complement(7812..8558)
                     /gene="mipA"
                     /locus_tag="PN977_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="scaffolding protein MipA"
                     /protein_id="HDI6042192.1"
                     /translation="MTKLKLLALGVLIATSAGVAHAEGKFSLGAGVGVVEHPYKDYDT
                     DVYPVPVINYEGDNFWFRGLGGGYYLWNDATDKLSITAYWSPLYFKAKDSGDHQMRHL
                     DDRKSTMMAGLSYAHFTQYGYLRTTLAGDTLDNSNGIVWDMAWLYRYTNGGLTVTPGI
                     GVQWNSENQNEYYYGVSRKESARSGLRGYNPNDSWSPYLELSASYNFLGDWSVYGTAR
                     YTRLSDEVTDSPMVDKSWTGLISTGITYKF"
     gene            8994..10928
                     /gene="yeaG"
                     /locus_tag="PN977_002164"
     CDS             8994..10928
                     /gene="yeaG"
                     /locus_tag="PN977_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YeaG"
                     /protein_id="HDI6042193.1"
                     /translation="MNIFDHYRQRYEAAKDEEFTLQEFLTTCRQDRSAYANAAERLLM
                     AIGEPVMVDTAQEPRLSRLFSNRVIARYPAFEEFYGMEDAIEQIVSYLKHAAQGLEEK
                     KQILYLLGPVGGGKSSLAERLKSLMQLVPIYVLSANGERSPVNDHPFCLFNPQEDAQI
                     LEKEYGIPRRYLGTIMSPCAAKRLHEFGGDITKFRVVKVWPSILQQIAIAKTEPGDEN
                     NQDISALVGKVDIRKLEHYAQNDPDAYGYSGALCRANQGIMEFVEMFKAPIKVLHPLL
                     TATQEGNYNGTEGISALPFNGIILAHSNESEWVTFRNNKNNEAFLDRVYIVKVPYCLR
                     ISEEIKIYEKLLNHSELTHAPCAPGTLETLSRFSILSRLKEPENSSIYSKMRVYDGES
                     LKDTDPKAKSYQEYRDYAGVDEGMNGLSTRFAFKILSRVFNFDHVEVAANPVHLFYVL
                     EQQIEREQFPQEQAERYLEFLKGYLIPKYAEFIGKEIQTAYLESYSEYGQNIFDRYVT
                     YADFWIQDQEYRDPDTGQLFDRESLNAELEKIEKPAGISNPKDFRNEIVNFVLRARAN
                     NSGRNPNWTSYEKLRTVIEKKMFSNTEELLPVISFNAKTSTDEQKKHDDFVDRMMEKG
                     YTRKQVRLLCEWYLRVRKSS"
     gene            11041..12324
                     /locus_tag="PN977_002165"
     CDS             11041..12324
                     /locus_tag="PN977_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaH/YhbH family protein"
                     /protein_id="HDI6042194.1"
                     /translation="MTWFIDRRLNGKNKSMVNRQRFLRRYKAQIKQSISEAINKRSVT
                     DVDSGESVSIPTEDISEPMFHQGRGGLRHRVHPGNDHFVQNDRIERPQGGGGGSGSGQ
                     GQASQDGEGQDEFVFQISKDEYLDLLFEDLALPNLKQNQQRQLTEYKTHRAGYTANGV
                     PANISVVRSLQNSLARRTAMTAGKRRELHALEEHLAIISNSEPAQLLEEERLRKEIAE
                     LRAKIERVPFIDTFDLRYKNYEKRPDPSSQAVMFCLMDVSGSMDQSTKDMAKRFYILL
                     YLFLSRTYKNVEVVYIRHHTQAKEVDEHEFFYSQETGGTIVSSALKLMDEVVKERYNP
                     AQWNIYAAQASDGDNWADDSPLCHEILAKKILPVVRYYSYIEITRRAHQTLWREYEHL
                     QSTFDNFAMQHIRDQDDIYPVFRELFHKQNATAKD"
     gene            12471..13946
                     /locus_tag="PN977_002166"
     CDS             12471..13946
                     /locus_tag="PN977_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HDI6042195.1"
                     /translation="MIRSTRISLGLFFNYFLSSTKIDPGQNYISLPSIKSSTHIALLF
                     MASMGTQKLKAQGFFVFSLLLTLILFCITTLYNENTNVKLIPQMNYLMVVVALFFLNA
                     VIFLFMLMKYFTNKQILPTLILSLAFLSGLIYLVETIVIIHKPINGSTLIQTKSNDVS
                     IFYIFRQLSFICLTSLALFCYGKDNILDNNKKKTGILLLALIPFLVFPLLAHNLSSYN
                     ADYSLYVVDYCPDNHTATWGINYTKILVCLWAFLLFFIIMRTRLASELWPLIALLCLA
                     SLCCNLLLLTLDEYNYTIWYISRGIEVSSKLFVVSFLIYNIFQELQLSSKLAVHDVLT
                     NIYNRRYFFNSVESLLSRPVVKDFCVMLVDINQFKRINAQWGHRVGDKVLVSIVDIIQ
                     QSIRPDDILARLEGEVFGLLFTELNSAQAKIIAERMRKNVELLSGFSNRYDVPEQMTI
                     SIGTVFSTGDTHNISLVMTEADKALREAKSEGGNKVIIHHI"
     gene            14127..15617
                     /locus_tag="PN977_002167"
     CDS             14127..15617
                     /locus_tag="PN977_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HDI6042196.1"
                     /translation="MKLHHRMLRHFIAASVIVLTSSFLIFELVASDRAMSAYLRYIVQ
                     RADSSFLYDKYQNQSIAAHVMRALAAEQSEVSPEQRRAICEAFESANNTHGLNLTAHK
                     YPGLRGTLQTASTDCDTIVEAAALLPAFDQAVEGNRHQDDYGSGLGMAEEKFHYYLDL
                     NDRYVYFYEPVNVEYFAMNNWSFLQSGSIGIDRKDIEKVFTGRTVLSSIYQDQRTKQN
                     VMSLLTPVYVAGQLKGIVLLDINKNNLRNIFYTHDRPLLWRFLNVTLTDTDSGRDIII
                     NQSEDNLFQYVSYVHDLPGGIRVSLSIDILYFITSSWKSVLFWILTALILLNMVRMHF
                     RLYQNVSRENISDAMTGLYNRKILTPELEQRLQKLVQSGSSVMFIAIDMDKLKQINDT
                     LGHQEGDLAITLLAQAIKQSIRKSDYAIRLGGDEFCIILVDSTPQIAAQLPERIEKRL
                     QHIAPQKEIGFSSGIYAMKENDTLHDAYKASDERLYVNKQNKNSRS"
     gene            15660..16163
                     /gene="yeaK"
                     /locus_tag="PN977_002168"
     CDS             15660..16163
                     /gene="yeaK"
                     /locus_tag="PN977_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mischarged aminoacyl-tRNA deacylase"
                     /protein_id="HDI6042197.1"
                     /translation="MTEMAKGSVTHQRLIALLSQEGANFRVVTHEAVGKCEAVSEIRG
                     TALGQGAKALVCKVKGNGVNQHVLAILAADQQADLSQLASHIGGLRASLASPAEVDEL
                     TGCVFGAIPPFSFHPKLKLVADPLLFERFDEIAFNAGMLDKSVILKTADYLRIAQPEL
                     VNFRRTA"
     gene            complement(16164..16268)
                     /locus_tag="PN977_002169"
     CDS             complement(16164..16268)
                     /locus_tag="PN977_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416302.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6042198.1"
                     /translation="MNDQMFVETLIITSSFFAIAAVLVLSVLLIERTG"
     gene            16438..16884
                     /locus_tag="PN977_002170"
     CDS             16438..16884
                     /locus_tag="PN977_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HDI6042199.1"
                     /translation="MFDVTLLILLGLAALGFISHNTTVAVSILVLIIVRVTPLSTFFP
                     WIEKQGLSIGIIILTIGVMAPIASGTLPPSTLIHSFLNWKSLVAIAVGVIVSWLGGRG
                     VTLMGSQPQLVAGLLVGTVLGVALFRGVPVGPLIAAGLVSLIVGKQ"
     gene            complement(16841..17662)
                     /gene="nimR"
                     /locus_tag="PN977_002171"
     CDS             complement(16841..17662)
                     /gene="nimR"
                     /locus_tag="PN977_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NimR"
                     /protein_id="HDI6042200.1"
                     /translation="MMHRLNLNGYEPDRHHEAAVAFCIHAGTDELTSPVHQHRKGQLI
                     LALHGAITCTVENALWMVPPQYAVWIPGGVKHSNQVTANAELCFLFIEPSAVTMPTTC
                     CTLKISPLCRELILTLANRTTTQRAEPMTRRLIQVLFDELPQQPQQQLHLPVSSHPKI
                     RTMVEMMAKEPVEWGSLGQWAGFFAMSERNLARLIVKETGLSFRQWRQQLQLIMALQG
                     LVKGDTVQKVAHTLGYDSTTAFITMFKKGLGQTPGRYIAGLTTVSPQSAKPDPRQ"
     gene            17759..18940
                     /gene="nimT"
                     /locus_tag="PN977_002172"
     CDS             17759..18940
                     /gene="nimT"
                     /locus_tag="PN977_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-nitroimidazole transporter"
                     /protein_id="HDI6042201.1"
                     /translation="MTCSTSLSGKNRIVLIAGILMIATTLRVTFTGAAPLLDTIRSAY
                     SLTTAQTGLLTTLPLLAFALISPLAAPVARRFGMERSLFAALLLICAGIAIRSLPSPY
                     LLFGGTAVIGGGIALGNVLLPGLIKRDFPHSVARLTGAYSLTMGAAAALGSAMVVPLA
                     LNGFGWRGALLMLMCFPLLALFLWLPQWRSQQHANLSTSRALHTRGIWRSPLAWQVTL
                     FLGINSLVYYVIIGWLPAILISHGYSEAQAGSLHGLLQLATAAPGLLIPLFLHHVKDQ
                     RGIAAFVALMCAVGAVGLCFMPAHAITWTLLFGFGSGATMILGLTFIGLRASSAHQAA
                     ALSGMAQSVGYLLAACGPPLMGKIHDANGDWSVPLMGVAILSLLMAIFGLCAGRDKEI
                     R"
     gene            18995..19342
                     /locus_tag="PN977_002173"
     CDS             18995..19342
                     /locus_tag="PN977_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HDI6042202.1"
                     /translation="MNIQCKRVYDPAEQSDGYRVLVDRLWPRGIKKTDLALDEWDKEI
                     TPSTELRKAFHGEVVDFATFREQYLAELAQHEQEGKRLANIAKKQPLTLLYSAKNTTQ
                     NHALVLADWLRSL"
     gene            complement(19364..19618)
                     /gene="yoaF"
                     /locus_tag="PN977_002174"
     CDS             complement(19364..19618)
                     /gene="yoaF"
                     /locus_tag="PN977_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing lipoprotein YoaF"
                     /protein_id="HDI6042203.1"
                     /translation="MKIISFVLPCLLVLAGCSTSSQPEAPKPPQIGMANPASVYCQQK
                     GGTLIPVQTAQGVSNNCKLPGGETIDEWALWRRDHPAGEK"
     gene            19801..20826
                     /gene="dgcP"
                     /locus_tag="PN977_002175"
     CDS             19801..20826
                     /gene="dgcP"
                     /locus_tag="PN977_002175"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416308.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcP"
                     /protein_id="HDI6042204.1"
                     /translation="MSDQIIARVSQSLAKEQSLESLVRQLLEMLEMVTDMESTYLTKV
                     DVEARLQHIMFARNSQKMHIPENFTVSWDYSLCKRAIDENCFFSDEVPDRWGDCIAAR
                     NLGITTFLSTPIHLPDGSFYGTLCAASSEKRQWSERAEQVLQLFAGLIAQYIQKEALV
                     EQLREANAALIAQSYTDSLTGLPNRRAIFENLTTLFSLARHLNHKIMIAFIDLDNFKL
                     INDRFGHNSGDLFLIQVGERLNTLQQNGEVIGRLGGDEFLVVSLNNENTDISSLRERI
                     QQQIRGEYHLGDVDLYYPGASLGIVEVDPETTDADSALHAADIAMYQEKKHKQKTPFV
                     AHPALHS"
     gene            20859..20957
                     /locus_tag="PN977_002176"
     CDS             20859..20957
                     /locus_tag="PN977_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaK family small membrane protein"
                     /protein_id="HDI6042205.1"
                     /translation="MRIGIIFPVVIFITAVVFLAWFFIGGYAAPGA"
     gene            20960..21034
                     /gene="yoaJ"
                     /locus_tag="PN977_002177"
     CDS             20960..21034
                     /gene="yoaJ"
                     /locus_tag="PN977_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YoaJ"
                     /protein_id="HDI6042206.1"
                     /translation="MKKTTIIMMGVAIIVVLGTELGWW"
     gene            complement(21093..21341)
                     /locus_tag="PN977_002178"
     CDS             complement(21093..21341)
                     /locus_tag="PN977_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HDI6042207.1"
                     /translation="MGILSWIIFGLIAGILAKWIMPGKDGGGFFMTILLGIVGAVVGG
                     WISTLFGFGKVDGFNFGSFVVAVIGAIVVLFIYRKIKS"
     gene            complement(21489..21671)
                     /locus_tag="PN977_002179"
     CDS             complement(21489..21671)
                     /locus_tag="PN977_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1869 domain-containing protein"
                     /protein_id="HDI6042208.1"
                     /translation="MGKATYTVTVTNNSNGVSVDYETETPMTLLVPEVAAEVIKDLVN
                     TVRSYDTENEHDVCGW"
     gene            complement(21675..22034)
                     /gene="yeaR"
                     /locus_tag="PN977_002180"
     CDS             complement(21675..22034)
                     /gene="yeaR"
                     /locus_tag="PN977_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1971 domain-containing protein"
                     /protein_id="HDI6042209.1"
                     /translation="MLQIPQNYIHTRSTPFWNKQTAPAGIFERHLDKGTRPGVYPRLS
                     VMHGAVKYLGYADEHSTEPDQVILIEAGQFAVFPPEKWHNIEAMTDDTYFNIDFFVAP
                     EVLMEGAQQRKVIHNGK"
     gene            complement(22207..22845)
                     /gene="leuE"
                     /locus_tag="PN977_002181"
     CDS             complement(22207..22845)
                     /gene="leuE"
                     /locus_tag="PN977_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine efflux protein LeuE"
                     /protein_id="HDI6042210.1"
                     /translation="MFAEYGVLNYWTYLVGAIFIVLVPGPNTLFVLKNSVSSGMKGGY
                     LAACGVFIGDAVLMFLAWAGVATLIKTTPILFNIVRYLGAFYLLYLGSKILYATLKGK
                     NNEAKSDEPQYGAIFKRALILSLTNPKAILFYVSFFVQFIDVNAPHTGISFFILATTL
                     ELVSFCYLSFLIISGAFVTQYIRTKKKLAKVGNSLIGLMFVGFAARLATLQS"
     gene            complement(22972..23895)
                     /gene="dmlR"
                     /locus_tag="PN977_002182"
     CDS             complement(22972..23895)
                     /gene="dmlR"
                     /locus_tag="PN977_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310535.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator DmlR"
                     /protein_id="HDI6042211.1"
                     /translation="MNNLPLLNDLRVFMLVARRAGFAAVAEELGVSPAFVSKRIALLE
                     QTLNVVLLHRTTRRVTITEEGERIYEWAQRILQDVGQMMDELSDVRQVPQGMLRIISS
                     FGFGRQVVAPALSALAKAYPQLELRFDVEDRLVDLVNEGVDLDIRIGDDIAPNLIARK
                     LATNYRILCASPEFIAQHGAPKHLTDLSALPCLVIKERDHPFGVWQLRNKEGHHAIKV
                     TGPLSSNHGEIVHQWCLDGQGIALRSWWDVSENIASGHLVQVLPEYYQPANVWAVYVS
                     RLATSAKVRITVEFLRQYFAEHYPNFSLEHA"
     gene            23998..25083
                     /gene="dmlA"
                     /locus_tag="PN977_002183"
     CDS             23998..25083
                     /gene="dmlA"
                     /locus_tag="PN977_002183"
                     /EC_number="1.1.1.83"
                     /EC_number="1.1.1.85"
                     /EC_number="1.1.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional
                     D-malate/3-isopropylmalate/tartarate dehydrogenase"
                     /protein_id="HDI6042212.1"
                     /translation="MMKTIRIAAIPGDGIGKEVLPEGIRVLKAAAERWGFALSFEQME
                     WASCEYYSHHGKMMPDDWHEQLSRFDAIYFGAVGWPDTVPDHISLWGSLLKFRREFDQ
                     YVNLRPVRLFPGVPCPLAGKQPGDIDFYVVRENTEGEYSSLGGRVNEGTEHEVVIQES
                     VFTRRGVDRILRYAFELAQSRPRKTLTSATKSNGLAISMPYWDERVEAMAENYPEICW
                     DKQHIDILCARFVMQPERFDVVVASNLFGDILSDLGPACTGTIGIAPSANLNPERTFP
                     SLFEPVHGSAPDIYGKNIANPIATIWAGAMMLDFLGNGDERFQQAHNGILAAIEEVIA
                     HGPKTPDMKGNATTPQVADAICKIILR"
     gene            25334..26944
                     /gene="yeaV"
                     /locus_tag="PN977_002184"
     CDS             25334..26944
                     /gene="yeaV"
                     /locus_tag="PN977_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310537.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BCCT family transporter YeaV"
                     /protein_id="HDI6042213.1"
                     /translation="MMSNVKKKDVPLISISLVAILFIAAALSLFPQQSADAANAIYTF
                     VTRTLGSAVQVLVLLAMGLVIYLATSKYGNIRLGEGKPEYSTLSWLFMFICAGLGSST
                     LYWGVAEWAYYYQTPGLNIAPRSQQALEFSVPYSFFHWGISAWATYTLASLIMAYHFH
                     VRKNKGLSLSGIIAAITGVRPQGPWGKLVDLMFLIATVGALTISLVVTAATFTRGLSA
                     LTGLPDNFTVQAFVILLSGGIFCLSSWIGINNGLQRLSKMVGWGAFLLPLLVLIVGPT
                     EFITNSIINAIGLTTQNFLQMSLFTDPLGDGSFTRNWTVFYWLWWISYTPGVAMFVTR
                     VSRGRKIKEVIWGLILGSTVGCWFFFGVMESYAIHQFINGVINVPQVLETLGGETAVQ
                     QVLMSLPAGKLFLAAYLGVMIIFLASHMDAVAYTMAATSTRNLQEGDDPDRGLRLFWC
                     VVITLIPLSILFTGASLETMKTTVVLTALPFLVILLVKVGGFIRWLKQDYADIPAHQV
                     EYYLPQTPVEALEKTPVLPAGTVFKGDN"
     gene            26976..28100
                     /gene="yeaW"
                     /locus_tag="PN977_002185"
     CDS             26976..28100
                     /gene="yeaW"
                     /locus_tag="PN977_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase subunit YeaW"
                     /protein_id="HDI6042214.1"
                     /translation="MSNLSPDFVLPENFCANPQEAWTIPARFYTDQNAFEHEKESVFA
                     KSWICVAHSSELANANDYVTREIIGESIVLVRGRDKVLRAFYNVCPHRGHQLLSGEGK
                     AKNVITCPYHAWAFKLDGNLAHARNCENVANFDSDKAQLVPVRLEEYAGFVFINMDPN
                     ATSVEDQLPGLGAKVLEACPEVHDLKLAARFTTRTPANWKNIVDNYLECYHCGPAHPG
                     FSDSVQVDRYWHTMHGNWTLQYGFAKPSEQSFKFEEGTDAAFHGFWLWPCTMLNVTPI
                     KGMMTAIYEFPVDSETTLQNYDIYFTNEELTDEQKSLIEWYRDVFRPEDLRLVESVQK
                     GLKSRGYRGQGRIMADSSGSGISEHGIAHFHNLLAQVFKD"
     gene            28156..29121
                     /gene="yeaX"
                     /locus_tag="PN977_002186"
     CDS             28156..29121
                     /gene="yeaX"
                     /locus_tag="PN977_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase subunit YeaX"
                     /protein_id="HDI6042215.1"
                     /translation="MSDYQMFEVQVNQIEPLTEQVKRFTLVATDGKPLPAFTGGSHVI
                     VQMSDGDNEYSNAYSLLSSPHDTSCYQIAVRLEENSRGGSRFLHQQVKVGDRLTISTP
                     NNLFALIPSARKHLFIAGGIGITPFLSHLAEMQYSDVDWQLHYCSRNPESCAFRDELV
                     QHPQAEKVHFHHSSTGTRLELARLLTDIEPGTHVYTCGPEALNEAVRSEAARLDIAVD
                     TMHFEQFAIEDKTGDAFTLVLARSGKEFVVPEEMTILQVIENNKAAKVECLCREGVCG
                     TCETAILEGEADHRDQYFSDEERASQQSMLICCSRAKGKRLVLDL"
     gene            complement(29168..30283)
                     /gene="rnd"
                     /locus_tag="PN977_002187"
     CDS             complement(29168..30283)
                     /gene="rnd"
                     /locus_tag="PN977_002187"
                     /EC_number="3.1.13.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HDI6042216.1"
                     /translation="MITTDDALASLCEAVRAFPAIALDTEFVRTRTYYPQLGLIQLFD
                     GEHLALIDPLGISDWSPLKAILRDPSITKFLHAGSEDLEVFLNVFGELPQPLIDTQIL
                     AAFCGRPMSWGFASMVEEYSGVTLDKSESRTDWLARPLTERQCEYAAADVWYLLPITA
                     KLMVETEASGWLPAALDECRLMQMRRQEVVAPEDAWRDITNAWQLRTRQLACLQLLAD
                     WRLRKARERDLAVNFVVREEHLWSVARYMPGSLGELDSLGLSGSEIRFHGKTLLALVE
                     KAQTLPEEALPQPMLNLMDMPGYRKAFKAIKSLITDVSETHKISAELLASRRQINQLL
                     NWHWKLKPQNNLPELISGWRGELMAEALHNLLQEYPQ"
     gene            complement(30365..32050)
                     /gene="fadD"
                     /locus_tag="PN977_002188"
     CDS             complement(30365..32050)
                     /gene="fadD"
                     /locus_tag="PN977_002188"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase FadD"
                     /protein_id="HDI6042217.1"
                     /translation="MKKVWLNRYPADVPTEINPDRYQSLVDMFEQSVARYADQPAFVN
                     MGEVMTFRKLEERSRAFAAYLQQGLGLKKGDRVALMMPNLLQYPVALFGILRAGMIVV
                     NVNPLYTPRELEHQLNDSGASAIVIVSNFAHTLEKVVDKTAVQHVILTRMGDQLSTAK
                     GTVVNFVVKYIKRLVPKYHLPDAISFRSALHNGYRMQYVKPELVPEDLAFLQYTGGTT
                     GVAKGAMLTHRNMLANLEQVNATYGPLLHPGKELVVTALPLYHIFALTINCLLFIELG
                     GQNLLITNPRDIPGLVKELAKYPFTAITGVNTLFNALLNNKEFQQLDFSSLHLSAGGG
                     MPVQQVVAERWVKLTGQYLLEGYGLTECAPLVSVNPYDIDYHSGSIGLPVPSTEAKLV
                     DDDDNEVPPGQPGELCVKGPQVMLGYWQRPDATDEIIKNGWLHTGDIAVMDEEGFLRI
                     VDRKKDMILVSGFNVYPNEIEDVVMQHPGVQEVAAVGVPSGSSGEAVKIFVVKKDPSL
                     TEESLVTFCRRQLTGYKVPKLVEFRDELPKSNVGKILRRELRDEARGKVDNKA"
     gene            complement(32255..32836)
                     /gene="yeaY"
                     /locus_tag="PN977_002189"
     CDS             complement(32255..32836)
                     /gene="yeaY"
                     /locus_tag="PN977_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Slp family lipoprotein YeaY"
                     /protein_id="HDI6042218.1"
                     /translation="MAVQKKVIKGILAGTFALMLSGCVTVPDAIKGSSPTPQQDLVRV
                     MSAPQLYVGQEARFGGKVVAVQNQQGKTRLEIATVPLDSGARPTLGEPSRGRIYADVN
                     GFLDPVDFRGQLVTVVGPITGTVDGKIGNTPYKFMVMQATGYKRWHLTQQVIMPPQPI
                     DPWFYGGRGWPYGYGGWGWYNPGPARVQTVVTE"
     gene            complement(32875..>33482)
                     /gene="tsaB"
                     /locus_tag="PN977_002190"
     CDS             complement(32875..>33482)
                     /gene="tsaB"
                     /locus_tag="PN977_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HDI6042219.1"
                     /translation="PREHTQRILPMVQDILTTSGTSLTDINALAYGRGPGSFTGVRIG
                     IGIAQGLALGADLPMIGVSTLMTMAQGAWRKNGATRVLAAIDARMGEVYWAEYQRDEN
                     GVWHGEETEAVLKPGLVHERLQQLSGEWVTVGTGWQAWPDLGKESGLALRDGDVLLPA
                     AEDMLPIACQMFAEGKTVAVEHAEPVYLRNNVAWKKLPGKE"
BASE COUNT         8493 a         8447 c         7922 g         8620 t
ORIGIN      
        1 aagaaatagc ttcaatcgtg accggataac gataggcata gcggaatacc gtctggatag
       61 tgacttcgcg attgattttg aggaaattga ttgccgaatc gcccggtaca gtaccaacaa
      121 tcataatttt accgccgcgc attaccagat aaggtgcctg tttaacggtg accgcagaac
      181 ctgctgtttc gaaaacaata tctgcgccca tttcttcggt aaattgctga cagcgtgcaa
      241 tagtgtcttc ttttgcgccg ttaataacca ctgtcgcgcc gagctgttcc gccattgtca
      301 gacgtttttc cagcacatca acgacggcaa tatccgtagc tcccaggcat ttgcacgctt
      361 gcaacgtcat caaaccaata caacctgctc ccagaataat tatcttctta cccggtttaa
      421 catctgccag catcgcggca tgcatcccga ctgcggcagg ctccaccagc gccccttcca
      481 tcgtgtccat attgtctggc agtttgtaag taaagctctc cggatgacac agatagtgcg
      541 ttaatgcgcc gcggtagttg ggttgtgtcg ccataaaatc aacgtccggg caaatgttat
      601 atttgccttc cagacagtaa cgacagtgac cgcaaggaac gccaggttcg atatttaccc
      661 gatcccccgg tttaaatttg cgcacgcggc ttcccacagc cacaaccgtc ccggcgcatt
      721 catgacccag gccaatttct tgatttgggt ctttaggcgg aataaacggg cctgattcaa
      781 aaccatgtac atctgaacca caaataccga catattctac tttaatcaaa acttcatctt
      841 ctttaggcac aggcatttct gctgaaataa ttttcattgt gcccggcacc tgcaatattg
      901 cttttgaatt tttcattttg aatccaccag tattatttat tgactactgc atcaatactt
      961 aaactttcaa cggagacacc tttggtttca attccaatgg tggcgatagc aattgcgaca
     1021 ataattgaaa ccgcccccag aagaataaag actcccgtta cgccataact actgagcagc
     1081 actgcaacgg cataaggtgc ggcaatacca ctgattcgcc ctactgcatt cgccagaccg
     1141 gagccacgta attttgcctc tgtcggccag atctcaggga catacactgc cgaggcatag
     1201 caaacgtaca tatagacgaa agtaatcagg aagaaaccaa tcagcgttat gagcaacata
     1261 ctggtttgca gtgaatagat atatccgagc accgcaatca ggattaatag ccccacaccc
     1321 attgttttac gcggaatttt atccatcacc agcatggcaa taaatatacc aaatggcgca
     1381 ccaaacatac tcatggtatt taaaacaatc gagtctttta aattaatccc ctgggtcatg
     1441 aatattgttg gcaaccagtt aattagtgtg tactgcacaa cgttcatggc aatcagcaca
     1501 caagaaccta atatcacgcg tttcagtaat actcctgtca gtaaggctga atacggcacc
     1561 gcttgtggcg ctttaccgtc atcagcaata accacgggcg gcaaaggttt tcccgtctgg
     1621 cgtatgacgc cttcttctat actccgcatg actttctcgg cttcctgata ccgtccgcgc
     1681 gattccagcc agcgcgggga ttcaggaaag tagcgccagg ccagcgccgt agcgataagc
     1741 gacaatattg cagggataag cagttgtact cgccagttcc actctgcact aatcagcggc
     1801 gtgagtccca tcgcaatcaa tgaacagagc ggatatgacc agttgccaat aaaagaaacc
     1861 cgactggacc acgttccgcg atttctaccg ggcatatatt cggtgaaacc agcaaacagc
     1921 gtcaccagta aagctcccag cccaacgccc atcacaaaac ggcaggcaat aaggaaatcc
     1981 atattcggtg aaaaagcacc gaccaccatc gaggcaatat gaatggcctc atagaggatg
     2041 aacgcatttc tgcgaccggt tttgtcaccg atgatgccgc caaccagcgc accgagaaac
     2101 attccggcgg tcgtgattgc tgagaatgtg gcagtggtgg aattatctgt ccagcccaac
     2161 gctttcagct gcgcgaggat caagccacca acggcattac tccagcagac aagcaagcca
     2221 aacgcgacga tagcaaacat tgacgaatgc cagcggcaat ccggtaagcg atccagccga
     2281 gcaccacaat gcggttttgt tatctgttcc atgcgataac gctccttcag gagagtagtt
     2341 attcatcaaa gtcgtaagtc atgatcactt tgattgcggt tttatcgacc atcgcatcaa
     2401 acccttcgcg ccattgcgac aggccgatac gatgcgtgat catcggtttg actttgatag
     2461 cgccgctggc taatagcctg atagcgttac gccatgaggt ggagtcatag gccatatgcc
     2521 caatgatgct tttgttccag gcggtaatgt cattaatcga gaaatcaaga ggtttgaagc
     2581 ccattccaac gcgtaccact tccccattcg ggcggagcat ttcgatggct tgtttcaatg
     2641 cgatattggc accggagcat tcaatcacca gccccagatt gtctttgcca caaatttgct
     2701 ggcagcgtgc caccacatct tcggtagaac catttactac tgccgtcgca cccagttctt
     2761 ttgcaaccgg gaagcggacc gccacatctt cttgtagacc aacgacgacg atatttaccg
     2821 cccccataat ccgcgccatt tgtacggaga acagtccgag tgggccagtg ccgatgacga
     2881 ctacatcctg accagggagg aatttcgatt gctgcgcgat ggatttgtag gcattgcaga
     2941 tagggtcaag tacggctgcg tcctcataat caacaccatc agggatttcc cacaacgcat
     3001 gacgatgaat tttgagaatt tcaccaggaa ccagacaata tttggaaaaa ccaccgcccc
     3061 aggtattgtt atccagacca aggtttactt tttctgtaca acacagaaaa tcaccttgtt
     3121 cacaggccgg acaaacgccg cagacgtgac cgctgttatc tgacacgacg cgttgcccca
     3181 ctttccagtc tttgactttt tcaccaacct gcgcaataca acctgcgaac tcatggccac
     3241 ggatagagtt aaactcatca gaaccgctat cgacattgta gtgcttcatg tctgcgccgc
     3301 agattgccgc ggctttaatt tcaatcacga catcttccgg gccacaaatg ggttgtggca
     3361 catcgatcat cttgtagccg ccaaaggctt tgccaaaccg agccagtgct ttcattgcgt
     3421 catcctctgt gttttgtttt gcttagatgg tcatcatact tacccacatg atttttgagg
     3481 tcaacattaa atctgtaaaa cacagataat gatctgcgtt ttacaactca gatcacaatt
     3541 acgcagaaaa tggctggagc tgttacaggt tgatggaagg cggaacgcaa aatgagcaaa
     3601 tttgagaggg attaccggcc aaataggtct gagacttaag ttcaggagaa gtcattcagg
     3661 cgtgaacgcg actgcaagag accggactgg ggctttgcct ggtcgtgacg agataattga
     3721 gatgagtcgt ggggttattg ttggctatcc cctttataac cgcggcaaaa aggggacaaa
     3781 ccacttactt aaacatggca attggctttg ctgagatacc aaaatcttct tttaactgtt
     3841 gtttgctctt catgaccatc tgaccatttg tgtcaattgt catatgctgt gcttcggtat
     3901 tatggcgggc ttgccacagc atcaccagtt gcaggcagtt ctctttttgt tcctcggtca
     3961 acgcaacgcc atcaggccat ttccccaatt caacggcggt cgacaaacgc tggtatacct
     4021 caggcgtcat gctgttgata atgtcatcaa gattcataat ttttccgctc ctgtggaata
     4081 agttgctgaa tcgttttttc aaccgttgat ttcttcgccg ttttcgccat cggtaaagcg
     4141 taaagaggca gagttaacac aataacgttc gcccgttggc tgcggcccgt cggggaagac
     4201 atgccccaga tgggcatcac agttaccgca acgaatttct atgcgctgca ttccatgtga
     4261 caagtctttg atataacgaa tggattcttc actcaccggt tcgtagaaac tgggccagcc
     4321 acagccggaa tcatacttgg tttgggaatg aaacagcggg gcatcgcaga tcaaacagtg
     4381 atatacgccg tcacgcttgt tatgcagtaa acgacccgta aatggcggct ctgtcccatg
     4441 attctgcgtc acgtagaact gcatctcgga caaatttttt ttcagttctt ctgccgaagg
     4501 tttattagcc atttgctcac atctcacttt aatcgtgctc acattacgtg actgattcta
     4561 acaaaacatt aacaccaact ggcaaaattt tgtcctaaac ttgatctcga cgaaatggct
     4621 gcacctaaat cgtgatgaaa atcacatttt tatcgtaatt gccctttaaa attcgggacg
     4681 ccgaccccat gtggtctcaa gcccaaagga agagtgaggc gagtcagtcg cgtaatgctt
     4741 aggcacagga ttgatttgtc gcaatgattg acacgattcc gcttgacgct gcgtaaggtt
     4801 tttgtaattt tacaggcaac cttttattca ctaacaaata gctggtggaa tatatgacta
     4861 tcaaagtagg tatcaacggt tttggccgta tcggtcgcat tgttttccgt gctgctcaga
     4921 aacgttctga catcgagatc gttgcaatca acgacctgtt agacgctgat tacatggcat
     4981 acatgctgaa atatgactcc actcacggcc gtttcgacgg taccgttgaa gtgaaagacg
     5041 gtcatctgat cgttaacggt aaaaaaatcc gtgttaccgc tgaacgtgat ccggctaacc
     5101 tgaaatggga cgaagttggt gttgacgttg tcgctgaagc aactggtctg ttcctgactg
     5161 acgaaactgc tcgtaaacac atcaccgctg gtgcgaagaa agtggttatg actggtccgt
     5221 ctaaagacaa cactccgatg ttcgttaaag gcgctaactt cgacaaatat gctggtcagg
     5281 acatcgtttc caacgcttcc tgcaccacca actgcctggc tccgctggct aaagttatca
     5341 acgataactt cggcatcatc gaaggtctga tgaccaccgt tcacgctact accgctactc
     5401 agaaaaccgt tgatggcccg tctcacaaag actggcgcgg cggccgcggt gcttcccaga
     5461 acatcatccc gtcctctacc ggtgctgcta aagctgtagg taaagtactg ccagaactga
     5521 atggcaaact gactggtatg gcgttccgcg ttccgacccc gaacgtatcc gtagttgacc
     5581 tgaccgttcg tctggaaaaa gctgcaactt acgagcagat caaagctgcc gttaaagctg
     5641 ctgctgaagg cgaaatgaaa ggcgttctgg gctacaccga agatgacgta gtatctaccg
     5701 atttcaacgg cgaagtttgc acttccgtgt tcgatgctaa agctggtatc gctctgaacg
     5761 acaacttcgt gaaactggta tcctggtacg acaacgaaac cggttactcc aacaaagttc
     5821 tggacctgat cgctcacatc tccaaataag ttgagatgac actgtgatct aaaaagagcg
     5881 acttcggtcg ctcttttttt tacctgataa aatgaagtta aaggactgcg tcatgattaa
     5941 gaaaattttt gcccttccgg tcatcgaaca aatctcccct gtcctctccc gtcgtaaact
     6001 ggatgaactg gacctcattg tggtcgatca tccccaggta aaagcctctt ttgcattaca
     6061 gggcgcacac cttctctcat ggaaacctgc gggtgaagaa gaagttctgt ggttgagcaa
     6121 taacacaccg ttcaaaaatg gcgtcgctat tcgcggtggc gtaccggttt gctggccgtg
     6181 gtttggtccg gctgcacaac aaggtctgcc tgcgcacggt tttgcccgca acctgccgtg
     6241 gacgctgaaa tcacatcgtg aagatgctga tggcgtagcg ctgacttttg aattgacaca
     6301 aagcgaagag acgaaaaaat tctggctaca cgactttacg ctattagcgc atttccgcgt
     6361 gggtaaagcc tgtgaaatcg atcttgaagc acacggcgaa tttgaaacca cctctgccct
     6421 gcatacctac tttaacgtgg gtgatatcac taaggtaagc gtcagtggac tgggcgatcg
     6481 cttcattgat aaagtgaatg acgcgaaaga agatgtactg accgacggta ttcagacctt
     6541 ccctgaccgt accgatcgcg tgtatctgaa tccgcaagat tgcagcgtaa ttaatgacga
     6601 agcgctgaat cgtattatcg ccgtaggcca ccagcatcat ctgaacgttg taggctggaa
     6661 cccaggaccg gcgctttcag ttagcatggg cgatatgccg gatgatggct acaagacctt
     6721 tgtttgtgta gaaacagctt acgcctcaga aacgcaaaaa gtcaccaaag agaaacctgc
     6781 acatctggcg caatccattc gcgttgcgaa acgttaattt gcgttaatgt tgtgtgccgg
     6841 gtgcgatgca tccggcacgt atcaacatca caccatatcc agcgcagttt ttccttttgg
     6901 tgccggatat gccttatcca gcatagctaa ttccgctgat gaaagttcga cctcaagcac
     6961 agccgcattt tgttggacat gggcaatcgt ggccgctttt ggaatcgcca tcacaccctg
     7021 atgactgatc acccacgcca acaatacttg tgccgcgctg atattgtgag catgtgcaat
     7081 ttcgttgact accgcgtttt ttaacagccc attgcgcaac cgcccggcct gggctaacgg
     7141 actgtaagcc atcaccggca tctgctgttg ctggcaccag ggaagtagat cgtactcaat
     7201 ccctcgtgaa ccgagatggt aaagcacctg attagtggca cactgattcc cccccggcag
     7261 ctgccagagt tcctgcatat cggcataatc aaggttagaa acgccccagc ggcggatttt
     7321 tccctgggcg atcaattttt ccatcgctgc gacagtctct tcaaaagcga aactgccaga
     7381 ccagtgtaat aagtagagat cgagataatc agtattgaga cggcgtaaac tggcttcgca
     7441 tgcatttatc gctttttgcc cgccagcatt ccacggatag actttagaga cgagaaagac
     7501 gttctcacgc aggccggtta atgcttcccc aaccaccatt tcggcaccgc catcggcata
     7561 catttcggcg gtatcaatga gggttaaacc gagttcaatg cccgcgcgta gtgcagcaac
     7621 ttctgtttta cgctgactgg catcttcgcc catataccat gttccctgcc ctatggctgg
     7681 cagtgagaca tcgccactaa attgaatcat tttttgttgc attgcttcct cccaggtatt
     7741 gcaccaccgt aatgcaaaac agggcatgac gccctgtttt tatgcacaaa atgccctgga
     7801 aagatgcatt atcagaattt gtaggtgatc ccggtagaaa tcaggccagt ccaggattta
     7861 tccaccattg ggctgtcagt aacttcatca gacagacggg tgtagcgcgc ggtaccgtaa
     7921 acactccagt cgccgaggaa gttgtagctg gcgctcagct ccaggtaagg gctccagctg
     7981 tcgttcgggt tatagccacg cagaccgctg cgagcggact ctttgcgcga tacgccataa
     8041 tagtattcgt tctggttttc gctgttccac tgcacaccaa tacccggagt cacggtcagg
     8101 ccgccgttgg tgtaacgata caaccaggcc atatcccaga caatgccgtt gctgttatcc
     8161 agggtatcgc cagccagggt ggtacgcagg taaccgtact gggtaaagtg agcataagac
     8221 agaccagcca tcatggtgct cttacggtca tccaggtgac gcatttggtg atcgccactg
     8281 tctttagctt tgaagtaaag cggcgaccag taagcggtaa ttgaaagttt gtccgttgcg
     8341 tcattccaca ggtagtaacc accacctaag ccacggaacc agaagttatc gccttcatag
     8401 ttgattaccg gtactgggta aacatcggta tcgtaatctt tatatgggtg ttcaacgaca
     8461 cctacgcctg cgcccaggga aaatttacct tcagcgtgcg ctacgcctgc agacgttgcg
     8521 ataagcactc caagtgccag aagtttgagt ttggtcacaa ttaatcattc cttaaacaaa
     8581 tgtttagcgg gcgacaaagt ttacccgtca atacatcgaa acccaacctt tttacgtttt
     8641 cattttttaa agtaactgtt taattttcct gacgcagatg acaccacgct tacagccaaa
     8701 tgaatttagc gttaccggca agcctggtct ttacattaat tatgcaaaat ttatggatga
     8761 gttgttgata tgccattgaa attaagaaag ccgtgcaggc aagttttcca tttgccatct
     8821 acgcttaatt ttgaaggtgt atcaccgggc acgttgttct catcgtcgat aaaatggcat
     8881 gagagttgct gtgttttagc aagagacgtc gttcagttta cctcttccgg gagcctctac
     8941 tattcatatg aacggctctt aacctgtgct aaaaaacgaa aggacggcat accatgaata
     9001 tattcgatca ctatcgccag cgatatgaag ctgccaagga cgaagagttc acactgcagg
     9061 agtttcttac cacttgtcgg caagatcgca gtgcttatgc caacgcggct gagcggctat
     9121 tgatggctat tggtgagcct gtcatggtcg atacagccca ggaacccaga ctttctcgac
     9181 tcttttctaa ccgggtcatt gcacgttatc cggcgtttga agagttttac ggcatggaag
     9241 acgcgattga acagattgtc tcttatctga aacacgcggc tcaggggctg gaagagaaga
     9301 aacaaatcct gtatctgctg gggcctgtgg gtgggggtaa atcatcgctt gctgagcgac
     9361 tgaaatcatt aatgcagctc gtgccgatct atgtattaag cgctaacggt gagcggagcc
     9421 cggtcaacga tcatccgttc tgtcttttca atccgcagga agatgcgcag attctggaaa
     9481 aagagtatgg cattcctcgc cgttatctcg gcaccatcat gtcgccgtgt gcggcaaaac
     9541 gcctgcatga atttggtggc gatatcacta agttccgggt agtgaaggtc tggccgtcaa
     9601 ttctgcaaca aattgctatc gccaaaacgg aacccggtga tgagaacaac caggacatct
     9661 ccgccctggt agggaaagtc gatattcgta aactcgaaca ctacgcgcag aatgacccgg
     9721 acgcctacgg ctattccggt gcgctgtgcc gcgccaatca ggggatcatg gaatttgtcg
     9781 agatgtttaa agcaccgata aaagtgctgc atcccttgtt aaccgccacc caggaaggta
     9841 actacaacgg gacggaaggt atctccgccc tgccgttcaa cgggattatt ctcgcgcact
     9901 cgaacgaatc cgaatgggtc actttccgta ataacaaaaa caacgaagcc ttcctcgacc
     9961 gtgtttacat cgtgaaggtg ccgtattgct tgcgcatttc cgaagagatc aaaatctacg
    10021 agaaattgct taatcacagt gaattgactc acgccccctg cgcccctggc acgctggaaa
    10081 cgctgtcacg tttttctatt ctttcgcgtc tgaaagagcc agaaaactcc agcatttatt
    10141 caaagatgcg ggtttatgat ggtgaaagtt tgaaagacac tgatcccaaa gccaagtcgt
    10201 atcaggaata tcgtgactac gccggtgtcg atgaagggat gaacggtctg tcgacgcgtt
    10261 ttgcgtttaa gatcctctcc cgcgtgttca acttcgatca tgtagaagtg gcggcaaacc
    10321 cggtccatct gttctacgtc ctggaacagc agatcgagcg cgagcagttc ccacaagagc
    10381 aggcagaacg ctatctggag ttcctgaaag gttatctgat cccgaaatat gccgagttta
    10441 tcggcaaaga gatccagacg gcctaccttg aatcctattc cgaatatggg caaaacattt
    10501 tcgaccgtta tgttacctac gcggatttct ggattcagga tcaggagtat cgcgatccgg
    10561 ataccgggca gttgtttgac cgcgagtctc ttaacgccga gctggagaaa atcgagaaac
    10621 cggcggggat cagtaatcca aaagatttcc gcaacgagat tgttaacttc gtactgcgcg
    10681 ccagagcgaa taacagcgga cgcaatccga actggaccag ctatgaaaaa ctgcgcacgg
    10741 ttattgagaa gaaaatgttc tccaataccg aggagctgtt gccggttatt tcgtttaacg
    10801 ccaaaacgtc aaccgacgag cagaagaaac atgacgactt tgtcgaccgt atgatggaaa
    10861 aaggctacac ccgtaaacag gtgcgtttac tgtgcgaatg gtatttgcgc gtacgtaaat
    10921 cgtcttaaca accctggccc ggtacacatg ctaccgggcc tacaacgaca gcgaaccgtg
    10981 ggccagagaa gcggcaacac aggcgtagca taccgttggc aaatgtagta cggggggcat
    11041 atgacctggt ttattgaccg gcgtctgaac ggcaaaaaca aaagcatggt gaatcgccag
    11101 cgttttttac gccgttataa agcgcaaatt aaacagtcga tctccgaggc cattaataag
    11161 cgttcggtga ctgacgtcga cagcggcgag tccgtatcca ttcccacgga agatattagc
    11221 gaaccgatgt ttcatcaggg gcgtggcggt ctgcgccacc gcgtgcatcc gggcaatgac
    11281 catttcgtcc agaacgaccg aattgaacgt ccccagggtg gcggcggagg ttccggcagt
    11341 ggtcagggcc aggccagcca ggatggtgaa ggtcaggatg aatttgtctt tcagatttcg
    11401 aaagatgagt atcttgatct gctctttgaa gatctggcct taccgaatct gaaacaaaac
    11461 caacaacgcc agctgaccga atataaaacg catcgtgcgg gttataccgc taacggcgtt
    11521 ccggccaata tcagcgttgt gcgttcattg cagaactcac tggcacgacg cacagccatg
    11581 acggcgggca agcggcggga acttcatgcg ctggaagagc atttggccat catcagcaac
    11641 agtgaacctg cgcaactgct ggaagaggaa cgtctgcgca aagaaattgc agaattacgt
    11701 gccaaaattg aacgcgtccc tttcattgac accttcgatt tacgttacaa gaactacgaa
    11761 aaacggcccg atccctccag ccaggcagtg atgttttgcc tgatggacgt ttcgggttca
    11821 atggatcagt ccactaaaga tatggctaag cgtttttata ttctgctgta tctgttcctt
    11881 agcagaacgt ataagaacgt ggaagtcgtt tacatccgcc accatactca ggcgaaagaa
    11941 gtcgatgaac atgagttttt ctactcgcag gaaactggcg gcaccattgt ttccagcgcc
    12001 ctgaaactga tggatgaggt agtgaaagag cgttataacc cggcacagtg gaatatttac
    12061 gcagcccaag catcagacgg cgataactgg gccgatgact ctccgctttg ccatgaaatt
    12121 ctggcgaaaa aaatattacc cgtcgttcgt tattacagct atatcgaaat tacacgtcgc
    12181 gcgcatcaga cactgtggcg agaatatgag catctgcaat ctactttcga caactttgcg
    12241 atgcagcata tccgcgacca ggatgatatt tatccggtgt tccgtgaact gtttcataaa
    12301 caaaatgcaa cagctaaaga ctaaaactgt cagccgggtc attatcgcct ggctgatttt
    12361 tagctcactg taaattatct catttattac atacattagc ttacaatcgc tttaaatatg
    12421 acagcataac ctttacacaa tttagttcca gaaaacaatc attcggaaaa atgattcggt
    12481 caacacgtat ttccctgggg ttattcttta attatttttt atcgtcaacg aaaattgatc
    12541 ctggtcaaaa ctatatatct ctgccatcaa taaaatccag cactcacatt gctctccttt
    12601 ttatggcttc tatgggtaca caaaaattaa aagctcaagg cttttttgtt ttcagcttat
    12661 tgctgacgtt aattttattt tgcattacta ctttatataa cgaaaacacg aatgtaaaac
    12721 tcatcccaca gatgaattac ctgatggttg ttgtggcttt gtttttcctt aacgccgtca
    12781 tttttctttt catgttaatg aagtatttca ctaacaaaca aattttacca acactcattt
    12841 taagccttgc atttttaagt ggccttatct atttagttga aaccattgta attatccata
    12901 aaccaatcaa cggcagtaca ctgatccaga caaagtcgaa tgatgtttct attttctata
    12961 ttttccgcca actcagtttc atttgtttaa cctcgctggc gctcttttgt tatgggaaag
    13021 acaacatcct tgacaacaat aagaaaaaaa ctggcattct gttgctggcg ctgattcctt
    13081 ttttagtttt tccccttctg gcacacaatc tgagtagtta taacgctgac tattctttgt
    13141 atgtcgtcga ttactgtccg gacaaccata ctgcgacctg gggaatcaac tatacaaaaa
    13201 tattggtttg tttgtgggca tttttactgt tctttattat catgcgtaca cgattagcca
    13261 gcgaactatg gccgttaata gcattattat gtctggcatc gctatgctgc aacttacttc
    13321 tactgactct ggatgagtat aattacacca tctggtatat cagtcgcggg attgaagttt
    13381 ccagtaaact gtttgttgtg tcttttctga tttataacat ttttcaggaa ctgcaactct
    13441 ccagcaaact ggcagttcat gatgtgctga ccaatattta taatcggcgc tactttttca
    13501 acagcgtaga gtcattattg tcgcgacctg ttgttaagga cttttgtgtc atgctggttg
    13561 atattaatca gttcaaacgc atcaatgccc aatggggaca tcgtgtgggt gataaagtgc
    13621 tggtatcaat tgtcgatatt atccagcaaa gcatccgccc cgatgatatt ttagcgcgac
    13681 tggagggtga ggtgtttggc ttgctattta ccgaactcaa tagtgcccag gcaaaaatca
    13741 ttgcggaacg tatgcgtaaa aatgtcgaac tcctgtccgg ctttagtaac agatatgatg
    13801 ttcctgaaca aatgaccatc agtattggca cggttttttc aacgggtgac acgcataata
    13861 tctcgcttgt catgacggaa gcagataaag ccttacgcga agcgaaaagc gaggggggca
    13921 acaaagtgat tattcatcat atttaagtgc aaaaatattc agagccatgc tttttgcgtg
    13981 gcttttgcat acaatttatt actattaccc ctaaatttcc cctccccagc ggcgcagaga
    14041 tgagtataat tagcgcccct gtgccaggcc gcaatcgaac tttatctggt tttctcgttt
    14101 cactaaccga aggagtgcca tttatcatga aattgcacca tagaatgctc cggcatttta
    14161 tcgccgcaag tgtcattgtg ctgacatctt ccttccttat ttttgaactt gtcgccagtg
    14221 acagagcaat gagtgcctat ctgcgctata tcgtgcagag agcagactcc tcctttcttt
    14281 atgataagta tcagaatcag agtattgccg cgcatgtgat gcgcgctctc gctgctgagc
    14341 agtcggaagt gtcgccagaa cagcggcgag ccatctgcga ggcttttgag tctgccaata
    14401 atacccatgg cttaaacctg actgcccata aataccctgg cttacgcggc acactacaaa
    14461 ccgcatccac tgactgcgac acaattgtgg aagctgctgc actattaccc gcttttgatc
    14521 aggcagtgga aggcaaccgc caccaggatg attacggttc aggtcttggg atggccgaag
    14581 agaaatttca ctattatctc gatctcaatg accgctatgt ctatttttat gagccggtta
    14641 atgttgagta ctttgcgatg aataactggt ccttcctgca gtcaggaagt attggcatcg
    14701 atcgcaaaga tattgaaaag gtatttaccg ggcgtaccgt attatcgagc atttaccagg
    14761 atcagcgtac taaacagaac gtgatgagtt tgctgacgcc ggtatatgtc gcagggcagc
    14821 taaaagggat tgtgctgctg gatattaaca aaaacaatct gcggaatatc ttttacactc
    14881 atgaccgccc tctcctctgg cgttttctca atgtcacgct aactgatacc gattcggggc
    14941 gcgacattat catcaaccag agcgaagata atctgttcca gtatgtcagt tacgtccatg
    15001 acttaccggg cggcattcgt gtctcgttat ccattgatat tctttacttt atcacgtctt
    15061 cgtggaaaag cgttctgttc tggattttga cggcgttaat tttgctgaat atggtgcgga
    15121 tgcacttccg tttataccaa aatgtgtcgc gagaaaatat tagtgatgcg atgactggac
    15181 tctataatcg caaaatttta acccctgaac tggagcagcg gttgcagaaa ctggtgcaat
    15241 ccggttcttc ggtgatgttt attgcaattg acatggacaa gttaaagcaa ataaatgaca
    15301 cccttggtca tcaggagggg gatttagcga ttacgttatt agctcaggcg attaaacaat
    15361 cgattcgtaa aagtgattat gccatccgac tcggtggcga tgaattctgc atcattcttg
    15421 tcgattcgac accgcaaatt gcagcacaac tgcctgaacg tatcgaaaaa cgtctgcaac
    15481 atatcgcgcc gcagaaagag atcggcttct cttccggtat ttacgcgatg aaagaaaacg
    15541 atacgttaca tgatgcgtat aaagcttccg atgagcgttt atatgtcaat aagcagaaca
    15601 aaaacagccg ttcatgataa ccttctgtag ttgtttgctt gtaatctcag gagcatgaaa
    15661 tgactgaaat ggctaaagga agcgtgacgc atcagcggtt aatcgcttta ttatcgcaag
    15721 aaggtgctaa cttccgcgtc gttacccatg aagcggtagg taaatgtgaa gcggtgtcag
    15781 aaattcgtgg caccgcttta ggccaggggg ctaaggcact ggtatgtaaa gtcaaaggca
    15841 atggcgtaaa tcaacatgtt ctggcgatcc tcgctgccga tcaacaggcc gatctgagcc
    15901 aactcgccag ccatataggc ggattacgcg cttcgttggc cagcccagcg gaagtcgatg
    15961 aactcacagg ctgtgtcttc ggcgcgatcc cccctttcag cttccatcca aaactcaaac
    16021 tggttgccga ccccctactc ttcgaacgat ttgatgaaat cgccttcaat gcaggcatgc
    16081 tggataaatc cgttattttg aaaaccgcgg attatttgcg tattgcgcaa ccagaactgg
    16141 tcaatttccg ccgcactgcg taactagcct gtccgttcaa taagcagaac ggacaaaacc
    16201 agcacagcag caatggcaaa aaacgatgac gtgataatca gtgtctcgac gaacatttga
    16261 tcgttcatag tatgcccccg ggagatagct gttttcacgt tattgttaac gtacacaaat
    16321 ggcagtttga tgacagttcg tctgattttt tatgcaaaaa acgtaaagat tttctacttc
    16381 ctgcctgcag caagcgtaaa gtaagcaggc ttattatttt ttggcaagga aaccacgatg
    16441 tttgatgtca ctttgctgat cctgctcggg ttagctgcgc tgggctttat cagtcataac
    16501 accactgtcg ccgtttcaat tctggtgttg atcattgtcc gcgtcacacc gttaagcacc
    16561 tttttcccgt ggattgaaaa acaggggcta agtatcggga taatcattct gacgattggc
    16621 gttatggctc ccatcgccag cgggacgcta ccgccctcaa ccctgattca ctcatttctg
    16681 aactggaaat cgctggtggc aattgcagtc ggcgttattg tctcctggct gggggggcgc
    16741 ggcgtgacgt taatgggcag ccagccgcaa ctggtcgccg ggctgctggt gggcactgtt
    16801 ttaggcgtgg cgctgtttcg cggcgtaccg gttggcccgc ttattgccgc gggtctggtt
    16861 tcgctgattg tggggaaaca gtagttaatc ccgcaatata tcggcctggc gtttgcccca
    16921 ggcctttctt aaacatagta ataaaggccg ttgttgaatc ataccccagc gtatgagcaa
    16981 ctttctgtac agtatcgcct ttcactaatc cctgaagcgc cataatcagc tgtaactgct
    17041 gacgccactg gcgaaaactc aaccccgtct ctttcactat cagccgggca agattgcgtt
    17101 cactcatcgc aaaaaagcca gcccattgcc ccaatgagcc ccactcgacg ggctctttcg
    17161 ccatcatctc caccatcgtg cggattttag gatgagaaga aacgggcagg tgcagctgtt
    17221 gttgtggctg ctgagggagt tcatcaaaca ggacctgaat taatctgcgg gtcatcggct
    17281 cagcacgctg agtcgtcgtc ctgtttgcca gggttaaaat taattcccgg cataagggcg
    17341 atattttcaa tgtgcaacat gttgtcggca ttgtcactgc gctgggctcg ataaacagaa
    17401 aacagagttc agcgttagct gtcacttgat tgctatgttt caccccgccc ggtatccaaa
    17461 ccgcatattg tggcggcacc atccacaaag cattttccac cgtacaggta attgcaccat
    17521 gtagcgccag tatcaactgc cctttgcggt gctgatgtac aggactggtt aactcatccg
    17581 ttccggcgtg aatacaaaat gcgaccgcgg cctcatggtg tcggtcgggt tcatagccat
    17641 tgagattcaa cctgtgcatc attttgtccg aatttagcga taatttgtca ttttagcttg
    17701 attcaacata acaataaaaa cggtaaggta cagcctcgtt tgtaacaatg agaagcatat
    17761 gacctgttca acttcattaa gcggcaaaaa caggattgtc cttatcgctg gtattctgat
    17821 gattgccaca acattacgcg tcacctttac cggcgcagca ccgttactgg atacgattcg
    17881 ttccgcttac tcgctgacga cagcgcaaac cggcttattg accaccctgc cattattggc
    17941 ctttgcgcta atctcacctt tggccgcccc ggtggcgcga cgttttggta tggaacgtag
    18001 cctgtttgcc gcgttacttt tgatctgtgc aggtatcgca attcgctctc tcccttcgcc
    18061 ttacttatta tttggcggta cagcggtcat tggcggtggg attgcattag gtaatgtctt
    18121 actgccagga ttaattaaac gcgatttccc tcattccgtc gccagactta ccggcgcata
    18181 ttccctgaca atgggagctg cggcggcact gggatcggct atggtagtgc cgctggcttt
    18241 gaacggtttt ggctggcgag gcgcgttgct catgctgatg tgttttcctc tgctggctct
    18301 ttttttatgg ctgccacagt ggcgaagtca acaacatgca aatttgagta cctcacgcgc
    18361 cttacatact cggggtatct ggcgttcgcc gcttgcctgg caggtaacat tgtttcttgg
    18421 gatcaactca ctcgtctatt acgtgattat tggctggctt ccggcgatcc tcatcagtca
    18481 cggctatagc gaagcacagg cgggttcact gcatggtttg ctgcaactag ccacagcagc
    18541 tcccggtttg ctgatcccac ttttcttaca tcatgtgaaa gatcagcgtg gtattgcagc
    18601 gttcgttgcc ttgatgtgcg cagtgggcgc ggttgggctc tgctttatgc cagcacacgc
    18661 gatcacctgg actctgcttt tcggttttgg ttccggcgca acaatgatac tggggttgac
    18721 gttcattggt ctgcgggcaa gttctgcgca tcaggcggcg gcactctcgg ggatggcaca
    18781 atccgtcggg tatttgctgg cagcctgtgg gccgccgctg atgggtaaaa tacacgatgc
    18841 taacggtgac tggtccgtac cactgatggg tgttgcaata ctttcactac tgatggcgat
    18901 tttcggactt tgcgccggga gagacaaaga aattcgctaa tatccggtgc tatagtgacg
    18961 tgacaaatca tgcgtgaaag ggagaacaaa cacgatgaat attcagtgca aacgcgttta
    19021 tgatccggct gaacagagcg atggttatcg cgtactggtc gaccgcctct ggccgcgcgg
    19081 tatcaaaaaa accgatttag cccttgatga gtgggataaa gaaatcacgc cgtcaacgga
    19141 actgcgcaaa gcctttcacg gcgaagtcgt cgattttgca acctttcgcg agcaatatct
    19201 tgcagaactg gcgcaacacg agcaagaagg aaagcggctg gcaaacatcg ccaaaaaaca
    19261 gccgctgacc ctgctctact cagcaaaaaa caccacgcag aaccatgcgc tggtgctggc
    19321 cgactggcta cgtagcttat gattttagta cagcatccgg cggttatttt tcaccagccg
    19381 gatgatcccg tcgccacaat gcccattcat caatcgtttc accgcccggt aatttgcaat
    19441 tgttgctgac cccttgcgct gtctgcactg gaatgagcgt cccgcccttc tgctggcaat
    19501 agaccgacgc cggatttgcc ataccaatct gcggcggttt aggtgcttct ggctgagaag
    19561 aggttgaaca accagccaga accagcaagc aaggcagaac aaaactgata attttcattt
    19621 attgatctca catatttatc caggcttaga gtatcgcggt atcgttttgt tttgcagcac
    19681 tatttttatt acattcactc aaatcatatt acgccttgtt tcatctttgt tgatgatgtt
    19741 ttatcatgcc tgcaaagatt aaataatcag catttacccg ccgtatcctg gagttgtttc
    19801 gtgtcagatc agattatcgc ccgcgtctcg caatcccttg ccaaagaaca gtcactggaa
    19861 agcctggtcc gacagcttct ggagatgctg gaaatggtca ctgatatgga atcaacctac
    19921 ctgaccaaag tggatgtcga agcgcgcctg cagcatataa tgtttgcccg taacagccag
    19981 aaaatgcaca tcccggagaa ttttaccgtc tcgtgggatt attcgttatg caaacgcgcc
    20041 attgatgaaa actgcttttt cagcgatgaa gtccccgacc gttggggcga ctgtattgcg
    20101 gcacgcaatc ttggcatcac cacatttctg agcacgccaa ttcacttacc ggatggatca
    20161 ttctatggca cgctttgcgc cgccagcagt gagaagcgcc agtggagtga acgcgcggaa
    20221 caggttttgc agttattcgc cggactgatt gcacaatata ttcaaaaaga ggcgctggta
    20281 gaacagctgc gcgaagccaa tgccgcactg atagcgcaat cgtataccga ctcgttaacc
    20341 gggctaccga atcggcgggc gatttttgaa aatctgacga cgctgttttc tctcgcccgg
    20401 catcttaacc ataagataat gatcgcgttt atcgacctgg ataacttcaa attaatcaac
    20461 gatcgttttg gtcataatag tggcgatctg tttctcattc aggttggcga gcgccttaat
    20521 acgctccagc aaaatggcga agttattggt cgtctcggcg gtgatgagtt tttggttgtt
    20581 tcactgaaca acgagaatac ggatatttcg tcgctgcgag aacgtattca acagcaaata
    20641 cgtggagaat atcacttagg tgatgttgat ttgtattatc ccggtgccag tcttggcata
    20701 gtagaagtcg atcctgaaac gaccgatgca gacagtgccc tgcatgctgc cgatatcgcg
    20761 atgtatcagg agaaaaaaca caaacagaaa acaccttttg tcgcgcatcc agcgctacat
    20821 tcctgaggcg tattcacatc cttttgattg gtgataacat gcgaatcggt attatttttc
    20881 cggttgtaat ctttattaca gcggtcgtat ttttagcatg gttttttatt ggcggctatg
    20941 ctgccccggg agcataaaga tgaaaaaaac aacgattatt atgatgggtg tggcgattat
    21001 tgtcgtactc ggcactgagc tgggatggtg gtaacgtcac atctagaaaa tagcaaaggc
    21061 tgcctgtgtg cagcctttgt gcaatttacg cgttaacttt taatctttct gtagataaac
    21121 agcacgacaa tcgcgccaat aacggcaacc acgaagctgc caaaattgaa gccatcgact
    21181 ttaccaaagc caaacagcgt gctgatccat ccgccgacta cggcaccgac tatccccagc
    21241 aggatagtca taaagaatcc accaccatct ttacctggca tgatccactt cgccagaata
    21301 ccggcaataa gcccaaaaat aatccatgac agaatgccca ttgtttcctc acttatctgt
    21361 tttgcattag cgggttagtc gctgataaaa agcatagcac aacatcggga gggcaagatt
    21421 tgtgacgagc atcacggagg atttttttgc gatggcgcag aaattgcgcc atcaacgatc
    21481 agtgataatt accaaccaca aacatcatgt tcgttttccg tgtcataaga acgcacggta
    21541 ttcaccagat cttttatcac ttcagccgcc acttctggca ccagcaaagt catcggcgtc
    21601 tctgtttcat aatcgacaga aacgccattg ctgttattgg tgacggtcac ggtatacgtt
    21661 gctttgccca tgattcattt cccgttatga atgactttcc gttgttgcgc accttccatc
    21721 aggacttcag gggcaacgaa gaagtcaatg ttgaaataag tatcgtcagt catggcttca
    21781 atgttgtgcc acttttctgg agggaacacc gcaaactgcc ccgcttcgat aaggatcact
    21841 tgatcaggct ctgtactgtg ttcatcagcg tagccgagat atttgaccgc cccatgcata
    21901 acggaaaggc gtgggtaaac ccccgggcgc gttcctttat caagatgacg ttcgaatatt
    21961 ccggcaggcg cagtttgttt attccagaaa ggcgttgagc gcgtatgaat ataattctgt
    22021 gggatttgca gcatcctttt ccctccttcg gtgaatgcgc tgaaaacggt ttatttcagc
    22081 cgtttcaggg tacgcctgat aatttgcatt ttaaatacca tttattggtt actatttagc
    22141 accgtatcag agaaaaatca gggaggatta cagatgggaa gcccgcgaca acgcgggctg
    22201 aaagcatcag gattgcagcg tcgccagtcg ggcggcgaaa cccacgaaca tcaaaccaat
    22261 cagtgagttg cccactttag ccagtttctt tttggtacgt atgtactgtg tgacaaaagc
    22321 accagaaata atcaggaagc tcaaatagca gaaactcacc agttccagcg tcgtcgccag
    22381 aataaagaat gaaattcccg tatgtggggc attaacatcg ataaactgta cgaaaaacga
    22441 cacatagaac aaaatggctt tcggattagt caggctcaaa attaacgcgc gtttaaaaat
    22501 ggcaccgtat tgaggctcat cggatttggc ctcgttattt ttacccttca gggtcgcgta
    22561 gagaatttta ctccccagat agagcaaata aaacgcacca agataacgta caatgttgaa
    22621 taatatcggg gtggtcttaa ttaatgtcgc cactccagcc catgccagaa acatcaatac
    22681 cgcatcgcca ataaatacac cacaggccgc aagataaccg cctttcatac cgctactgac
    22741 gctatttttg agtacaaaca gggtatttgg ccctggcacc aacacaataa aaatggcccc
    22801 aaccagatag gtccagtaat tcagaacccc gtattcagcg aacacattaa cctctttaat
    22861 tatctttcga tcatgcgcga ttaaaggtga atatgctaac caatctgtag ctgcttagaa
    22921 aggagaaaat caggttttaa cccgatatca acgcgataat tgaatcatta atcaggcatg
    22981 ctccagtgaa aaattcgggt agtgctcggc aaaatactgg cgtaaaaatt ctaccgttat
    23041 ccgcactttc gctgacgtcg ccagccttga aacataaacg gcccagacgt tcgctggctg
    23101 gtaatattcc ggtagcactt gcactaaatg accactggca atgttttcgc taacatccca
    23161 ccaggagcgc agagcaatcc cctgcccatc gagacaccac tgatggacaa tttctccatg
    23221 attagacgac agtgggccgg taactttaat cgcatggtgg ccttctttat tgcgtaattg
    23281 ccagacacca aaaggatgat cgcgctcttt aatgaccagg cacggcaatg cagaaagatc
    23341 ggttaaatgt ttcggcgcgc catgttgcgc aataaattcc ggtgacgcgc agagaatgcg
    23401 ataattggtc gccagtttgc gggcgattaa attaggggcg atatcatcgc caatgcgaat
    23461 atcgagatcg acaccttcat tgaccaaatc gaccaaccgg tcttccacat caaaacgtaa
    23521 ttccagttgc ggatacgcct tcgccagagc tgataacgcc ggagccacca cctgtcgtcc
    23581 aaaaccaaag ctgctgataa tacgcagcat cccctgtggc acctgacgca cgtcagaaag
    23641 ttcgtccatc atctgaccga catcctgcaa aatccgctgc gcccattcat aaatccgctc
    23701 tccttcttcg gtaatggtga cgcggcgggt ggtgcggtgt aacaacacca cgtttagcgt
    23761 ttgctccagc aaggcgatgc gcttgctgac gaacgccgga gaaacgccca attcttctgc
    23821 cacggcggca aaaccagccc ggcgagccac cagcataaag acgcgcaaat cgttcagcag
    23881 cggtaaatta ttcatgattc gtgttttatg tttcaccagt tacagggatt aattcctttt
    23941 cagtcaatta taggatggtg atgttgtcaa ttttgatggt caggaagtga gaacccaatg
    24001 atgaaaacga tccgtattgc tgcgatcccg ggagacggga ttggcaaaga agtccttcct
    24061 gaagggattc gcgtgttaaa ggctgccgct gagcgctggg gcttcgcctt gagttttgag
    24121 caaatggagt gggcgagctg cgagtattac agccatcacg gtaaaatgat gccggacgac
    24181 tggcatgagc aacttagccg tttcgacgcc atctattttg gtgccgtcgg ctggccggat
    24241 accgttccgg accatatttc gttgtggggt tcgctgctga aatttcgtcg tgagttcgac
    24301 cagtacgtca acctgcgccc ggttcgtctc tttcctggcg ttccctgccc gctggcggga
    24361 aaacagcctg gcgacatcga tttttacgtg gtcagggaaa acaccgaagg agaatattcc
    24421 tcgctcggcg gtagagtgaa tgaaggtaca gagcatgaag tcgtcattca ggaatcggta
    24481 tttacgcgtc gtggtgtcga tcgcattttg cgttatgcct ttgaacttgc acaaagccgc
    24541 ccacgtaaga cgctaacttc tgcaactaaa tcgaacggtt tagccatcag catgccgtac
    24601 tgggatgagc gagtggaagc aatggccgag aattacccgg agatctgctg ggacaagcag
    24661 catattgata ttctctgcgc gcgttttgtg atgcagccgg aacgcttcga tgtggtggtg
    24721 gcgtccaatt tgtttggcga tatcctttcc gatcttggcc cggcctgcac cggcaccatt
    24781 ggcattgccc catccgccaa cctgaatccg gaacgcactt tcccgtcgct cttcgagcca
    24841 gtccacggtt ccgcgccgga tatctacggg aaaaacattg ctaaccctat cgccacaatt
    24901 tgggccggtg caatgatgct cgattttctc ggcaatggcg atgagcgttt ccagcaagcg
    24961 cataacggta ttctggccgc gattgaagaa gtgattgctc acgggccgaa aacacctgat
    25021 atgaaaggca atgccaccac gccacaggtt gccgacgcga tttgcaaaat tattttgcgt
    25081 taaggtcaaa ccagtttatt tgaaccgcgt cactgacgcg gtttttttat tcgttctttg
    25141 cagtaaataa cctgcgtcat ttcacctttt attgtttccg tttcgtgttt tatggctttc
    25201 cgtattctta attgtttaat ttatgtaaca tgcaaataat caatttgtta atcacgacat
    25261 acaactcatt accctgcaaa taattacaac tggcaaaagc cagagacggg cttttatgcc
    25321 caggagatga ctcatgatga gcaatgttaa gaaaaaagat gtgccgctga taagcatcag
    25381 tctggtggcc attcttttca tcgcagctgc attaagcctt ttcccacaac aatcggccga
    25441 cgcggccaac gctatataca cttttgttac gcgtacgtta ggttccgccg tacaggtatt
    25501 ggttttgctg gcaatgggac tggtgattta tttagccacc agtaaatacg gcaatattcg
    25561 tcttggcgaa ggaaaaccgg aatacagcac gctctcctgg ctgtttatgt ttatttgtgc
    25621 cggtttaggt tcttctacgc tttattgggg ggttgctgaa tgggcctatt attatcaaac
    25681 acctggatta aatatcgcac cgcgttcaca acaggcactc gaatttagcg tcccttactc
    25741 cttcttccac tggggcatca gcgcctgggc aacttatacg ctggcctcat taatcatggc
    25801 ttatcacttt catgtgcgga aaaacaaagg tctgagtctt tccggcatta ttgccgcgat
    25861 taccggcgtt cgcccgcaag gcccgtgggg aaaactggtc gatttgatgt tcctgatcgc
    25921 cactgtcggc gcactgacca tttcccttgt tgtcaccgca gcaaccttta cccgtgggct
    25981 ttccgcgctg accggtttac ccgataattt caccgtgcag gcatttgtga tcctgctttc
    26041 cggcggcatt ttttgcctaa gctcatggat tggtatcaac aacggtttgc aacgtctgag
    26101 caaaatggtt ggctggggcg cgttcctgct gccattactg gtgctcattg tcggcccaac
    26161 cgaatttatt accaacagca tcatcaatgc catcggcctg accacgcaaa acttcctgca
    26221 aatgagctta ttcaccgatc cgcttggcga tggttcattt acccgcaact ggaccgtttt
    26281 ctactggctg tggtggatct catacacccc tggcgtagca atgtttgtca cccgcgtttc
    26341 ccgcggtcgt aagattaaag aagttatctg gggactgatc ctcggcagca ccgtcggttg
    26401 ctggttcttc ttcggcgtaa tggaaagcta tgccattcac cagtttatca atggcgtaat
    26461 caacgtccca caggtgctgg aaacactggg cggcgagaca gctgtgcagc aagttctgat
    26521 gtcgttgcca gccggtaaat tgttcctcgc cgcatacctg ggcgtgatga ttattttcct
    26581 tgcctcgcat atggatgcag tggcctacac catggcggcg accagtacgc gtaatctcca
    26641 ggaaggtgac gatcctgacc gtgggctgcg tcttttctgg tgcgtggtaa ttaccctgat
    26701 cccgctttca attcttttca ctggcgcgtc gctggaaacg atgaaaacca ccgtcgtact
    26761 cacagccctt ccgttcctcg tcattttact ggtgaaagtc ggcggattta ttcgctggct
    26821 gaaacaggat tacgccgaca ttccggctca tcaagttgaa tattatctcc cgcagacacc
    26881 ggttgaagcc ctggaaaaaa caccagtgct ccctgcggga accgtattca aaggcgacaa
    26941 ctgagcgcca tcatcctaac gataaaggta tccctatgag caatctgagc cctgactttg
    27001 tactacccga aaatttttgc gctaacccgc aagaggcgtg gaccattcct gcccgttttt
    27061 ataccgatca gaacgccttt gaacacgaaa aagagagcgt cttcgccaaa agctggattt
    27121 gcgtcgctca cagcagcgaa ctggcgaatg ccaatgatta tgtgacgcgt gagatcattg
    27181 gcgaaagcat tgtgctggta cgcggtcgtg ataaggtttt gcgcgcgttc tataacgtgt
    27241 gtccgcaccg tggtcatcag ttgttgagcg gtgaaggaaa agcgaaaaat gtgattacct
    27301 gcccatatca cgcatgggca ttcaaactcg atggcaacct ggcccatgca cgtaactgcg
    27361 aaaacgtcgc caatttcgat agcgacaaag cgcaactggt tccggtgcgt ctggaagaat
    27421 atgccggatt cgtcttcatc aacatggacc ctaacgccac cagcgtagaa gatcagttac
    27481 ccggtctggg ggcgaaagtg ctggaagcct gcccggaagt ccacgacctg aaactggcgg
    27541 cccgctttac cacccgcacg cctgccaact ggaagaacat tgtcgataac tatctcgagt
    27601 gctatcactg tggtccggcg catccaggtt tctccgactc cgtacaggtt gatcgttact
    27661 ggcacaccat gcacggtaac tggacgctgc aatacggttt cgccaaaccg tccgaacagt
    27721 ctttcaaatt cgaagagggc acggatgcgg cattccacgg tttctggctg tggccgtgca
    27781 cgatgctgaa cgtcacccca atcaaaggga tgatgacggc catttatgaa ttcccagtgg
    27841 attctgaaac taccctgcaa aactacgata tttacttcac caatgaagag ttaaccgacg
    27901 agcaaaaatc gctgattgag tggtatcgcg atgtgttccg tccggaagat ttacgtctgg
    27961 ttgaaagcgt acagaaaggg ctgaaatcgc gtggctatcg tggtcagggg cgcatcatgg
    28021 ccgacagtag cggtagcgga atttccgaac atggtatcgc ccatttccat aatctgctgg
    28081 cgcaggtgtt taaggactaa tgacatcagc ggcggtattt tccgccgctg ggctgatttt
    28141 tgatggagta cagcaatgtc agactatcaa atgtttgaag tacaggtgaa ccaaattgaa
    28201 ccgcttaccg aacaggtgaa acgcttcacg ctggtggcaa ccgatggcaa accattacct
    28261 gcgtttaccg gaggaagtca cgtcattgtg cagatgagcg atggtgataa cgagtacagc
    28321 aatgcgtatt cactactgag ttcgccgcat gacacctctt gttatcagat tgccgttcgg
    28381 ctggaggaaa actcgcgcgg cggttcccgc tttttgcatc agcaggtaaa agtgggcgat
    28441 cggttaacga tttcaacgcc taataacctg tttgcgctaa ttccctcggc cagaaagcat
    28501 ctgtttatcg cgggaggcat tggtatcacc cctttcctgt cgcacctggc agagatgcaa
    28561 tacagcgacg tcgactggca gttacattac tgctcacgaa atccagaaag ttgcgcattt
    28621 cgtgatgagc tagtccagca tccgcaggct gagaaagttc attttcatca ctcatcaacc
    28681 ggaacacgac tggaattagc gcgattattg acggatatcg aacctggcac acatgtttat
    28741 acctgtggcc ccgaggcgct aaatgaggcg gtaagaagtg aagctgcgcg tctggacatc
    28801 gccgtcgata cgatgcactt tgaacaattt gcgattgaag acaaaaccgg cgatgcattt
    28861 accctggtgc ttgcccgttc cggtaaagag tttgtggtgc cggaagagat gactattttg
    28921 caggttattg aaaataataa agcagcgaaa gtggaatgtt tatgtcgtga aggggtatgc
    28981 ggaacctgcg aaacggcaat actggaaggt gaagctgacc atcgggatca gtattttagc
    29041 gatgaagagc gtgccagcca gcaaagtatg ttgatctgtt gttcgcgcgc gaagggtaaa
    29101 cgcctggtgt tggatttgta gtttgccgaa gccggactga gcgcgctcag tccggcttcg
    29161 gaagatttta ctgcggatat tcctgcaata aattgtgtaa tgcttccgcc atcagctcac
    29221 cacgccagcc ggaaatcagc tccggcaaat tgttctgcgg tttcagtttc cagtgccagt
    29281 tcagcagttg gttgatttgc cgacgcgatg ccagcaattc ggcgctgatc ttgtgcgttt
    29341 cgctcacgtc agtaatcagc gacttaatcg ctttaaacgc tttacgataa cccggcatgt
    29401 ccatcaggtt aagcatcggc tgcggtaagg cctcttccgg caatgtctgc gctttttcca
    29461 ccagcgccag cagcgtttta ccgtgaaagc ggatttcgct accggataaa cccaggctgt
    29521 ccagttcgcc taaacttccc ggcatataac gcgctacaga ccacaaatgc tcttcacgca
    29581 cgacaaagtt cactgccaga tcgcgctccc gcgccttgcg cagtcgccaa tcggctaaca
    29641 gttgcagaca ggccagttgg cgtgtgcgta actgccaggc attggtgata tcacgccagg
    29701 catcttccgg cgcaacgact tcctgacgac gcatttgcat caggcggcat tcatccagcg
    29761 ccgcaggtag ccagccggag gcctccgttt ctaccataag cttggcggtg atcggtaaca
    29821 aataccagac atccgccgct gcgtattcac actgacgttc ggtcagcggt ctggccagcc
    29881 agtcggtgcg cgattcactc ttgtccagcg taacgccgga atactcttcc accatggaag
    29941 caaaacccca tgacatcggg cgtccgcaga aggcagcaag gatttgcgtg tcaatcaggg
    30001 gttgtggtaa ttcgccaaag acattgagga acacttccag atcttcactg cccgcatgga
    30061 gaaattttgt gatggacgga tcgcgcagga tcgctttcag tggtgaccag tcggagatcc
    30121 cgagaggatc aattagcgcc agatgctcac catcaaaaag ttgaattaga cctagctgcg
    30181 ggtaataggt gcgcgtacga acaaattcag tatccagggc tatcgccgga aaggcacgga
    30241 cggcttcaca caaagaagcc agcgcatcgt ccgtggtaat catttggtaa ttcaaattgt
    30301 attctcttta gtgggcgtca aaaaaaacgc cggattaacc ggcgtctgac gactgactta
    30361 acgctcaggc tttattgtcc actttgccgc gcgcttcgtc acgtaattct cgtcgcaaaa
    30421 tttttccgac gttagatttc ggtaactcat cacgaaactc caccagcttc ggtactttgt
    30481 agcccgtgag ctgacggcgg caaaaagtca ccagtgactc ttcggtaagc gatggatctt
    30541 ttttcacaac gaagattttc accgcttcac cactagagcc ggaaggtacg ccaacagccg
    30601 cgacttcctg tacgccagga tgctgcatga cgacatcttc aatctcgttg ggatagacgt
    30661 taaaaccgga aaccagaatc atgtcttttt tacgatcgac aatgcgcagg aatccttctt
    30721 catccatcac cgcgatgtcg ccggtgtgta accagccatt tttgatgatt tcatcggtag
    30781 catccggacg ctgccagtaa cccagcatca cctgcggtcc tttgacacaa agctcacctg
    30841 gttgaccagg tggtacttca ttatcatcat catccaccag tttggcttcc gtcgacggca
    30901 ccggcaaacc gatgctacca ctatgataat caatatcata cgggttaacg ctcaccagcg
    30961 gcgcacactc ggtcaggcca taaccttcca gcagatactg tccagtcagt ttcacccaac
    31021 gttctgccac cacttgctga acaggcatcc ctccgcctgc ggaaagatgc agactggaga
    31081 aatccagctg ctggaactct ttattgttca gcaacgcatt gaacaaggtg ttaacgccag
    31141 tgatagcggt aaacggatat ttcgctaact cttttaccaa ccctggaata tcgcgcgggt
    31201 tagtgataag caggttctgc ccccccagtt cgataaacag caggcagtta atggtcagtg
    31261 caaaaatgtg atacagcggc agcgccgtca ccaccagctc tttgcccgga tgcaacagcg
    31321 gaccataggt cgcgttaacc tgttccaggt tcgccagcat attgcggtga gtcagcatcg
    31381 cgcctttcgc cacaccagtg gtaccgccgg tgtattgcag gaaagctaaa tcttccggca
    31441 ccagttcggg tttgacgtac tgcatccggt agccgttgtg cagggcgcta cgaaatgaaa
    31501 tggcatctgg cagatggtat ttcggcacca gacgcttgat gtatttaaca acgaaattga
    31561 ctaccgtgcc ttttgccgta gatagctgat cgcccatacg ggtcagaatt acgtgctgaa
    31621 cggcggtttt atcaaccact ttttccagag tgtgggcaaa gttagacacg ataacaatcg
    31681 ccgatgcgcc gctatcgtta agctgatgct caagttcacg cggggtatac aacgggttaa
    31741 cgtttacgac aatcatcccg gcacgcaaaa tgccaaacag cgccaccgga tattgcaata
    31801 aattaggcat catcaacgca acgcgatcgc ctttcttcag tcccaaccct tgttgcaaat
    31861 aagcggcaaa cgcgcgactg cgttcttcca gtttgcggaa ggtcatcacc tcccccatat
    31921 tcacaaacgc gggttgatcg gcgtagcgcg cgaccgactg ctcaaacata tctaccagag
    31981 attgataacg gtcagggttg atctccgtcg gaacgtccgc gggataacgg ttaagccaaa
    32041 ccttcttcaa ttcttcacct ctaaaatgcg tgttcgtcgt catcgcaacc ccaaatgata
    32101 tacatgccgt taacataata ttaactcatc ataccagctt gataattacc caacgaaaag
    32161 gttgcgaagc gcgtcactat ttatttttat ctttaccgta agaatgcaga aacagcggac
    32221 cagccgctgt ttctttttct ttaaaaacaa gcaattattc agttacaaca gtttgtactc
    32281 tcgcggggcc gggattatac cagccccatc caccgtagcc atagggccag ccacgaccgc
    32341 cataaaacca tggatcaatc ggctgaggcg gcataatcac ctgctgggtt aaatgccaac
    32401 gtttgtaacc tgttgcctgc atcaccataa atttataggg cgtgttgccg atttttccgt
    32461 caaccgtacc ggtgattggc ccgacaactg tcaccagctg cccacggaaa tcgaccggat
    32521 caagaaaacc attcacatcg gcataaatgc gaccgcgaga aggttctccc agcgtcggtc
    32581 tggctccgct gtccagtggt acggtggcaa tttccaggcg ggttttccct tgctggtttt
    32641 gtaccgcaac cactttgcca ccaaagcgtg cctcctgacc aacgtacagc tgcggcgcac
    32701 tcatcacccg aactaaatct tgttgcggcg tgggactgct gcctttaatg gcgtccggca
    32761 cagtaacaca accgctgagc attagcgcaa acgtacctgc cagtatgcct ttgataactt
    32821 tcttttgaac cgccatgatg cgactccttt tttctcaggg catactctta agattcattc
    32881 tttgcccgga agtttcttcc atgcgacgtt gttacgtaaa taaaccggtt cggcatgttc
    32941 caccgccacc gttttaccct cggcaaacat ctgacacgca atcggcagca tatcttcggc
    33001 ggcaggcagt aacacatcgc catcgcgcag ggccaacccg ctctctttac cgagatccgg
    33061 ccaggcttgc cagcccgtac ccacggttac ccattcgccg gaaagctgtt gcaaccgttc
    33121 atgaacgagt cctggtttca gtacggcttc tgtttcttcg ccgtgccaga cgccgttttc
    33181 atcgcgctga tattcggccc agtaaacttc ccccattcgc gcgtcaatgg ctgccagcac
    33241 gcgggttgcg ccgtttttgc gccacgcccc ttgcgccatg gtcatgagcg tggaaacgcc
    33301 aatcatgggc agatccgcgc caagcgccag cccctgcgca ataccaatac caatgcgcac
    33361 gccggtaaag ctaccggggc cgcgcccgta tgccagagca tttatgtcag tcagggaagt
    33421 tccgctggtg gtcaggatat cctgcaccat cggtaaaatt cgttgagtat gttcacgagg
    33481 gc
//
LOCUS       DAMZFT010000045        33108 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.045, whole genome shotgun sequence.
ACCESSION   DAMZFT010000045 DAMZFT010000000
VERSION     DAMZFT010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 33108)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33108)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33108
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.045"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..384
                     /locus_tag="PN977_002191"
     CDS             <1..384
                     /locus_tag="PN977_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HDI6042220.1"
                     /translation="LSERPVMNMVENMLDQAFKKLNPHEHPVLHSDQGWQYRMRRYQN
                     ILKEHGIKQSMSRKGNCLDNAVVECFFGTLKSECFYLDEFSNISELKDAVTEYIEYYN
                     SRRISLKLKGLTPIEYRNQTYMPRV"
     gene            complement(662..2731)
                     /gene="glyS"
                     /locus_tag="PN977_002192"
     CDS             complement(662..2731)
                     /gene="glyS"
                     /locus_tag="PN977_002192"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HDI6042221.1"
                     /translation="MSEKTFLVEIGTEELPPKALRSLAESFAANFTAELDNAGLAHGT
                     VQWFAAPRRLALKVANLAEAQPDREIEKRGPAIAQAFDAEGKPSKAAEGWARGCGITV
                     DQAERLTTDKGEWLLYRAHVKGESTEALLPNMVATSLAKLPIPKLMRWGASDVHFVRP
                     VHTVTLLLGDKVIPATILGIQSDRVIRGHRFMGESEFTIDNADQYPEILRERGKVIAD
                     YEERKAKIKADAEEAARKIGGNADLSESLLEEVASLVEWPVVLTAKFEEKFLAVPSEA
                     LVYTMKGDQKYFPVYANDGKLLPNFIFVANIESKDPQQIISGNEKVVRPRLADAEFFF
                     NTDRKKRLEDNLPRLQTVLFQQQLGTLRDKTDRIQALAGWIAEQIGADVNHATRAGLL
                     SKCDLMTNMVFEFTDTQGVMGMHYARHDGEAEDVAVALNEQYQPRFAGDDLPSNPVAC
                     ALAIADKMDTLAGIFGIGQHPKGDKDPFALRRAALGVLRIIVEKNLNLDLQTLTEEAV
                     RLYGDKLTNANVVDDVIDFMLGRFRAWYQDEGYTVDTIQAVLARRPTRPADFDARMKA
                     VSHFRTLDAAAALAAANKRVSNILAKSDEVLSDRVNASTLKEPEEIKLAMQVVVLRDK
                     LEPYFAEGRYQDALVELAELREPVDAFFDKVMVMVDDKELRINRLTMLEKLRELFLRV
                     ADISLLQ"
     gene            complement(2741..3652)
                     /gene="glyQ"
                     /locus_tag="PN977_002193"
     CDS             complement(2741..3652)
                     /gene="glyQ"
                     /locus_tag="PN977_002193"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HDI6042222.1"
                     /translation="MQKFDTRTFQGLILTLQDYWARQGCTIVQPLDMEVGAGTSHPMT
                     CLRALGPEPMAAAYVQPSRRPTDGRYGENPNRLQHYYQFQVVIKPSPDNIQELYLGSL
                     KELGMDPTIHDIRFVEDNWENPTLGAWGLGWEVWLNGMEVTQFTYFQQVGGLECKPVT
                     GEITYGLERLAMYIQGVDSVYDLVWSDGPLGKTTYGDVFHQNEVEQSTYNFEYADVDF
                     LFTCFEQYEKEAQQLLALENPLPLPAYERILKAAHSFNLLDARKAISVTERQRYILRI
                     RTLTKAVAEAYYASREALGFPMCNKDK"
     gene            complement(3747..4055)
                     /locus_tag="PN977_002194"
     CDS             complement(3747..4055)
                     /locus_tag="PN977_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YsaB family lipoprotein"
                     /protein_id="HDI6042223.1"
                     /translation="MMMNSFFPAMALMVLMALVGCSTPPPVQKAQRVKVDPLRSLNME
                     GLCKDQAAKRYNTGAQKIDVTAFEQFQGSYEMRGYTFRKEQFVCSFDADGHFLHLSMR
                     "
     gene            4230..5225
                     /gene="wecH"
                     /locus_tag="PN977_002195"
     CDS             4230..5225
                     /gene="wecH"
                     /locus_tag="PN977_002195"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyltransferase WecH"
                     /protein_id="HDI6042224.1"
                     /translation="MQPKIYWIDNLRGIACLMVVMIHTTTWYVTNAHSVSPVTWDIAN
                     VLNSASRVSVPLFFMISGYLFFGERSAQPRHFLRIGLCLLFYSTVALLYIALFTSINM
                     ELALKNLLQKPVFYHLWFFFAIAVIYLVSPLIQVKNVGGKMLLVLMVVIGIIANPNTV
                     PQKIDGFEWLPINLYINGDTFYYILYGMLGRAIGMMDTQHKALSWVCAALFATGVFII
                     SRGTLYELQWRGNFADTWYLYCGPMVFICAITLLTLVKNTLDTHTVPGLGLISRHSLG
                     IYGFHALIIHALRTRGIELKNWPILDIIWIFCATLAASLLLSMLVQRIDRNRLVS"
     gene            complement(5267..5704)
                     /locus_tag="PN977_002196"
     CDS             complement(5267..5704)
                     /locus_tag="PN977_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiaB family inner membrane protein"
                     /protein_id="HDI6042225.1"
                     /translation="MDNKISTYSPAFSIVSWIALVGGIVTYLLGLWNAEMQLNEKGYY
                     FAVLVLGLFSAASYQKTVRDKYEGIPTTSIYYMTCLTVFIISVALLLVGLWNATLLLS
                     EKGFYGLAFFLSLFGAVAVQKNIRDAGINPPKETQVTQEEYSE"
     gene            complement(5750..6091)
                     /locus_tag="PN977_002197"
     CDS             complement(5750..6091)
                     /locus_tag="PN977_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6042226.1"
                     /translation="MKTSKTVAKLLFVVGALVYLVGLWISCPLLSGKGYFLGVLMTAT
                     FGNYAYLRAEKLGQLDDFFIRVCQLLALITIGLLFIGVLNAPINAYEMVIYPIAFFVC
                     LFGQMRLFRSA"
     gene            complement(6260..7714)
                     /gene="xylB"
                     /locus_tag="PN977_002198"
     CDS             complement(6260..7714)
                     /gene="xylB"
                     /locus_tag="PN977_002198"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HDI6042227.1"
                     /translation="MYIGIDLGTSGVKVILLNEQGEVVAAQTEKLTVSRPHPLWSEQD
                     PEQWWQATDRAMKALGVQHSLQDVKALGIAGQMHGATLLDAQQRVLRPAILWNDGRCA
                     QECTLLEARVPQSRVITGNLMMPGFTAPKLLWVHRHEPEIFRQIDKVLLPKDYLRLRM
                     TGEFASDMSDAAGTMWLDVAKRDWSDLMLQACHLSRDQMPALYEGSEITGALLPEVAK
                     AWGMATVPVVAGGGDNAAGAVGVGMVDANQAMLSLGTSGVYFAVSEGFLSKPESAVHS
                     FCHALPQRWHLMSVMLSAASCLDWAAKLTGLSNVPALIAAAQQADESTEPVWFLPYLS
                     GERTPHNNPQAKGVFFGLTHQHGANELARAVLEGVGYALADGMDVVHACGIKPQSVTL
                     IGGGARSEYWRQMLADISGQQLDYRTGGDVGPALGAARLAQIATNPEKSLIELLPQLP
                     LEQSHLPDAQRYAAYQPRRETFRRLYQQLLPLMA"
     gene            complement(7786..9108)
                     /gene="xylA"
                     /locus_tag="PN977_002199"
     CDS             complement(7786..9108)
                     /gene="xylA"
                     /locus_tag="PN977_002199"
                     /EC_number="5.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
                     /protein_id="HDI6042228.1"
                     /translation="MQAYFDQLDRVRYEGSKSSNPLAFRHYNPDELVLGKRMEEHLRF
                     AACYWHTFCWNGADMFGVGAFNRPWQQPGEALALAKRKADVAFEFFHKLHVPFYCFHD
                     VDVSPEGASLKEYINNFAQMVDVLAGKQEESGVKLLWGTANCFTNPRYGAGAATNPDP
                     EVFSWAATQVVTAMEATHKLGGENYVLWGGREGYETLLNTDLRQEREQLGRFMQMVVE
                     HKHKIGFQGTLLIEPKPQEPTKHQYDYDAATVYGFLKQFGLEKEIKLNIEANHATLAG
                     HSFHHEIATAIALGLFGSVDANRGDAQLGWDTDQFPNSVEENALVMYEILKAGGFTTG
                     GLNFDAKVRRQSTDKYDLFYGHIGAMDTMALALKIAARMIEDGELDKRIAQRYSGWNS
                     ELGQQILKGQMSLADLAKYAQEHNLSPVHQSGRQEQLENLVNHYLFDK"
     gene            9474..10466
                     /gene="xylF"
                     /locus_tag="PN977_002200"
     CDS             9474..10466
                     /gene="xylF"
                     /locus_tag="PN977_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter substrate-binding
                     protein"
                     /protein_id="HDI6042229.1"
                     /translation="MKIKNILLTLCTSLLLTNVAAHAKEVKIGMAIDDLRLERWQKDR
                     DIFVKKAESLGAKVFVQSANGNEETQMSQIENMINRGVDVLVIIPYNGQVLSNVVKEA
                     KQEGIKVLAYDRMINDADIDFYISFDNEKVGELQAKALVDIVPQGNYFLMGGSPVDNN
                     AKLFRAGQMKVLKPYVDSGKIKVVGDQWVDGWLPENALKIMENALTANNNKIDAVVAS
                     NDATAGGAIQALSAQGLSGKVAISGQDADLAGIKRIAAGTQTMTVYKPITLLANTAAE
                     IAVELGNGQEPKADTTLNNGLKDVPSRLLTPIDVNKNNIKDTVVKDGFHKESEL"
     gene            10544..12085
                     /gene="xylG"
                     /locus_tag="PN977_002201"
     CDS             10544..12085
                     /gene="xylG"
                     /locus_tag="PN977_002201"
                     /EC_number="7.5.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter ATP-binding protein"
                     /protein_id="HDI6042230.1"
                     /translation="MPYLLEMKNITKTFGSVKAIDNVSLRLNAGEIVSLCGENGSGKS
                     TLMKVLCGIYPHGSYEGEIIFAGEEIQASHIRDTERKGIAIIHQELALVKELTVLENI
                     FLGNEITHNGIMDYDLMTLRCQKLLAQVSLSISPDTRVGDLGLGQQQLVEIAKALNKQ
                     VRLLILDEPTASLTEQETSVLLDIIRDLQQHGIACIYISHKLNEVKAISDTIFVIRDG
                     QHIGTRDAAGMSEDDIITMMVGRELTALYPNEPHTTGDEILRIEHLTAWHPVNRHIKR
                     VNDVSFSLKRGEILGIAGLVGAGRTETIQCLFGVWPGQWEGKIYIDGKQVDIRNCQQA
                     IAQGIAMVPEDRKRDGIVPVMAVGKNITLAALNKFTGGISQLDDAAEQKCILESIQQL
                     KVKTSSSDLAIGRLSGGNQQKAILARCLLLNPRILILDEPTRGIDIGAKYEIYKLINQ
                     LVQQGIAVIVISSELPEVLGLSDRVLVMHEGKLKANLINHNLTQEQVMEAALRSEHHV
                     EKQSV"
     gene            12063..13244
                     /gene="xylH"
                     /locus_tag="PN977_002202"
     CDS             12063..13244
                     /gene="xylH"
                     /locus_tag="PN977_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylose ABC transporter permease XylH"
                     /protein_id="HDI6042231.1"
                     /translation="MSKSNPSEVKLAVPTSGGFSGLKSLNLQVFVMIAAIIAIMLFFT
                     WTTDGAYLSARNVSNLLRQTAITGILAVGMVFVIISAEIDLSVGSMMGLLGGVAAICD
                     VWLGWPLPLTIIVTLVLGLLLGAWNGWWVAYRKVPSFIVTLAGMLAFRGILIGITNGT
                     TVSPTSAAMSQIGQSYLPASTGFIIGALGLMAFVGWQWRGRMRRQALGLQSPASTAVV
                     GRQALTAIIVLGAIWLLNDYRGVPTPVLLLTLLLLGGMFMATRTAFGRRIYAIGGNLE
                     AARLSGINVERTKLAVFAINGLMVAIAGLILSSRLGAGSPSAGNIAELDAIAACVIGG
                     TSLAGGVGSVAGAVMGAFIMASLDNGMSMMDVPTFWQYIVKGAILLLAVWMDSATKRR
                     S"
     gene            13322..14500
                     /gene="xylR"
                     /locus_tag="PN977_002203"
     CDS             13322..14500
                     /gene="xylR"
                     /locus_tag="PN977_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator XylR"
                     /protein_id="HDI6042232.1"
                     /translation="MFTKRHRITLLFNANKAYDRQVVEGVGEYLQASQSEWDIFIEED
                     FRARIDKIKDWLGDGVIADFDDKQIEQALADVDVPIVGVGGSYHLAESYPPVHYIATD
                     NYALVESAFLHLKEKGVNRFAFYGLPESSGKRWATEREYAFRQLVAEEKYRGVVYQGL
                     ETAPENWQHAQNRLADWLQTLPPQTGIIAVTDARARHILQVCEHLHIPVPEKLCVIGI
                     DNEELTRYLSRVALSSVAQGARQMGYQAAKLLHRLLDKEEMPLQRILVPPVRVIERRS
                     TDYRSLTDPAVIQAMHYIRNHACKGIKVDQVLDAVGISRSNLEKRFKEEVGETIHAMI
                     HAEKLEKARSLLISTTLSINEISQMCGYPSLQYFYSVFKKAYDTTPKEYRDVNSEVML
                     "
     gene            complement(14608..15432)
                     /locus_tag="PN977_002204"
     CDS             complement(14608..15432)
                     /locus_tag="PN977_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein bax"
                     /protein_id="HDI6042233.1"
                     /translation="MILTPIRRYGAMILMLLTLVFSSEVLAKTHTTTASQKSHLTKAS
                     NKQVSSKQEYSRNSAKSSSLPDLRKYPSGTPRKKAFLRTVMPYITSQNAAITAERNWL
                     ISKQYQGQWSPAERARLKDIAKRYKVKWSGNTRKIPWNTFLERVDIIPTSMVATMAAA
                     ESGWGTSKLARNNNNLFGMKCMKGRCTNAPGKVKGYSQFSSVKESVSAYVTNLNTHPA
                     YSSFRKSRAQLRKADQEVTATAMIHKLKGYSTKGKSYNNYLFAMYQDNQRLIAAHM"
     gene            15752..17782
                     /gene="malS"
                     /locus_tag="PN977_002205"
     CDS             15752..17782
                     /gene="malS"
                     /locus_tag="PN977_002205"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HDI6042234.1"
                     /translation="MKLAACFLTLLPGFAVAASWTSPGFPAFSEQGTGTFVSHAQLPK
                     GTRPLTLNFDQQCWQPADAIKLNQMLSLQPCSNTPPQWRLFRDGEYTLQLDTRSGTPT
                     LMISIQNAVEPVASLVRECPKWDGLPLTLDVSATFPEGAAVRDYYSQQIAIVKNGQIT
                     LQPAATSNGLLLLERAETDASAPFDWHNATVYFVLTDRFENGDPSNDQSYGRHKDGMA
                     EIGTFHGGDLRGLTNKLDYLQQLGVNALWISAPFEQIHGWVGGGTKGDFPHYAYHGYY
                     TQDWTNLDANMGREADLRTLVDSAHQRGIRILFDVVMNHTGYATLADMQEFQFGALYL
                     SGDEVKKTLGERWSDWKPAAGQTWHSFNDYINFSDKTGWDKWWGKNWIRTDIGDYDNP
                     GFDDLTMSLAFLPDIKTESTTASGLPVFYKNKTDTHAKVIDGFTPRDYLTHWLSQWVR
                     DYGIDGFRVDTAKHVELPAWQQLKTEASAALREWKKANSDKALDDKPFWMTGEAWGHG
                     VMQSDYYRHGFDAMINFDYQEQAAKAVDCLAQMDTTWQQMAEKLQDFNVLSYLSSHDT
                     RLFREGGNKAAELLLLAPGAVQIFYGDESSRPFGPTGSDPLQGTRSDMNWQDVSGKSA
                     ASVAHWQKISQFRARHPAIGEGKQTTLSMKQGYGFVREHGDDKVLVIWAGQQ"
     gene            17960..19213
                     /gene="avtA"
                     /locus_tag="PN977_002206"
     CDS             17960..19213
                     /gene="avtA"
                     /locus_tag="PN977_002206"
                     /EC_number="2.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--pyruvate transaminase"
                     /protein_id="HDI6042235.1"
                     /translation="MTFSLFGDKFTRHSGITLLMEDLNDGLRTPGAIMLGGGNPAQIP
                     EMQDYFQTLLTDMLESGKATDALCNYDGPQGKTELLTLLAGMLREKLGWDIEPQNIAL
                     TNGSQSAFFYLFNLFAGRRADGRIKKVLFPLAPEYIGYADAGLEEDLFVSARPNIELL
                     PEGQFKYHVDFEHLHIGEETGMICVSRPTNPTGNVITDEELLKLDALANQHGIPLVID
                     NAYGVPFPGIIFSEARPLWNPNIVLCMSLSKLGLPGSRCGIIIANEKIITAITNMNGI
                     ISLAPGGIGPAIMCEMIKRNDLLRLSETVIKPFYYQRVQETIAIIRRYLPEDRCLIHK
                     PEGAIFLWLWFKDLPITTEQLYQRLKARGVLMVPGHNFFPGLDKPWPHTHQCMRMNYV
                     PEPDKIEAGVKILAEEIERAWAESH"
     gene            complement(19365..19838)
                     /locus_tag="PN977_002207"
     CDS             complement(19365..19838)
                     /locus_tag="PN977_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HDI6042236.1"
                     /translation="MNRFIIADATKCIGCRTCEVACAVSHQENQDCAALSPDEFISRI
                     RVIKDHSWTTAVACHQCEDAPCANVCPVDAISREHGHIFVEQSRCIGCKSCMLACPFG
                     AMEVVSSRKKARAIKCDLCWHRATGPACVEACPTKALQCMDVEKVQRHRLRQQSV"
     gene            complement(19940..20788)
                     /gene="yiaJ"
                     /locus_tag="PN977_002208"
     CDS             complement(19940..20788)
                     /gene="yiaJ"
                     /locus_tag="PN977_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator YiaJ"
                     /protein_id="HDI6042237.1"
                     /translation="MGKEVMGKKENEMAQEKERPAGSQSLFRGLMLIEILSNYPNGCP
                     LAHLSELAGLNKSTVHRLLQGLQSCGYVTTAPAAGSYRLTTKFIAVGQKALSSLNIIH
                     IAAPHLEALNIATGETINFSSREDDHAILIYKLEPTTGMLRTRAYIGQHMPLYCSAMG
                     KIYMAFGHPDYVKSYWESHQHEIQPLTRNTITELPAMFDELAHIRESGAAMDREENEL
                     GVSCIAVPVFDIHGRVPYAVSISLSTSRLKQVGEKNLLKPLRETAQAISNELGFTVRD
                     DQGAIT"
     gene            20989..21987
                     /gene="yiaK"
                     /locus_tag="PN977_002209"
     CDS             20989..21987
                     /gene="yiaK"
                     /locus_tag="PN977_002209"
                     /EC_number="1.1.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydro-L-gulonate 2-dehydrogenase"
                     /protein_id="HDI6042238.1"
                     /translation="MKVTFEQLKAAFNRVLISRGVDSETADACAEMFARTTESGVYSH
                     GVNRFPRFIQQLENGDIIPDAQPKRITSLGAIEQWDAQRSIGNLTAKKMMDRAIELAA
                     DHGIGLVALRNANHWMRGGSYGWQAAEKGYIGICWTNSIAVMPPWGAKECRIGTNPLI
                     VAIPSTPITMVDMSMSMFSYGMLEVNRLAGRQLPVDGGFDDEGNLTKEPGVIEKNRRI
                     LPMGYWKGSGMSIVLDMIATLLSDGASVAEVTQDNSDEYGVSQIFIAIEVDKLIDGPT
                     RDAKLQRIMDYVTTAERADENQAIRLPGHEFTTLLAENRRNGITVDDSVWAKIQAL"
     gene            21999..22466
                     /locus_tag="PN977_002210"
     CDS             21999..22466
                     /locus_tag="PN977_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HDI6042239.1"
                     /translation="MIFGHIAQPNPCRLPAAIEKALDFLRTIDFNALEPGVVEIDGKN
                     IYAQIIDLTTREAVENRPEVHRRYIDIQFLAWGEEKIGIAIDTGNNKVSESLLEQRDI
                     IFYHDSEHESFIEMIPGSYAIFFPQDVHRPGCILQTASEIRKIVVKVALTALN"
     gene            22584..23057
                     /gene="yiaM"
                     /locus_tag="PN977_002211"
     CDS             22584..23057
                     /gene="yiaM"
                     /locus_tag="PN977_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diketo-L-gulonate TRAP transporter small
                     permease YiaM"
                     /protein_id="HDI6042240.1"
                     /translation="MKKILEAILAINLAVLSCIVFINIILRYGFQTSILSVDELSRYL
                     FVWLTFIGAIVAFMDNAHVQVTFLVEKLSPAWQRRVALVTHSLILFICGALAWGATLK
                     TIQDWSDYSPILGLPIGLMYAACLPTSLVIAFFELRHLYQLITRSNSLTSPPQGA"
     gene            23060..24337
                     /gene="yiaN"
                     /locus_tag="PN977_002212"
     CDS             23060..24337
                     /gene="yiaN"
                     /locus_tag="PN977_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diketo-L-gulonate transporter large permease
                     YiaN"
                     /protein_id="HDI6042241.1"
                     /translation="MAVLIFLGCLLGGIAIGLPIAWALLLCGAALMFWLGMFDVQIMA
                     QTLVNGADSFSLLAIPFFVLAGEIMNAGGLSKRIVDLPMKLVGHKPGGLGYVGVLAAM
                     IMASLSGSAVADTAAVAALLVPMMRSANYPVNRAAGLIASGGIIAPIIPPSIPFIIFG
                     VSSGLSISKLFMAGIAPGMMMGATLMLTWWWQASRLNLPRQQKATMQEIWHSFVSGIW
                     ALFLPVIIIGGFRSGLFTPTEAGAVAAFYALFVATVIYREMTFATLWHVLIGAAKTTS
                     VVMFLVASAQVSAWLITIAELPMMVSDLLQPLVDSPRLLFIVIMLAILIVGMVMDLTP
                     TVLILTPVLMPLVKEAGIDPIYFGVMFIINCSIGLITPPIGNVLNVISGVAKLKFDDA
                     VRGVFPYVLVLYSLLVVFVFIPDLIILPLKWIN"
     gene            24350..25336
                     /gene="yiaO"
                     /locus_tag="PN977_002213"
     CDS             24350..25336
                     /gene="yiaO"
                     /locus_tag="PN977_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diketo-L-gulonate transporter
                     substrate-binding protein YiaO"
                     /protein_id="HDI6042242.1"
                     /translation="MKLRSVTYALFIAGLAAFSTSSMAAQSLRFGYETSQTDSQHIAA
                     KKFNDLLQERTKGELKLKLFPDSTLGNAQAMISGVRGGTIDMEMSGSNNFAGLSPVMN
                     LLDVPFLFRDTAHAHKTLDGKVGDDLKASLEGKGLKVLAYWENGWRDVTNSRAPVKTP
                     ADLKGLKIRTNNSPMNIAAFKVFGANPIPMPFAEVYTGLETRTIDAQEHPINVVWSAK
                     FYEVQKYLSLTHHAYSPLLVVINKAKFDGLTPEFQQALISSAQEAGNYQRKLVAEDQQ
                     KIIDGMKEAGVEVITDLDRKAFSDALGNQVRDMFVKDVPQGADLLKAVDEVQ"
     gene            25340..26836
                     /gene="lyxK"
                     /locus_tag="PN977_002214"
     CDS             25340..26836
                     /gene="lyxK"
                     /locus_tag="PN977_002214"
                     /EC_number="2.7.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-xylulokinase"
                     /protein_id="HDI6042243.1"
                     /translation="MTQYWLGLDCGGSWLKAGLYDREGREAGVQRLPLCALSPQPGWA
                     ERDMAELWQCCMAVIRTLLTHSGVSGEQIVGIGISAQGKGLFLLDKNDKPLGNAILSS
                     DRRAMEIVRRWQEDGIPEKLYPLTRQTLWTGHPVSLLRWLKEHEPERYAQIGCVMMTH
                     DYLRWCLTGVKGCEESNISESNLYNMSLEEYDPCLTDWLGIAEINHALPPVVGSAEIC
                     GEITAQTAALTGLKAGTPVVGGLFDVVSTALCAGIEDEFTLNAVMGTWAVTSGITRGL
                     RDGEAHPYVYGRYVNDGQFIVHEASPTSSGNLEWFTAQWGEISFDEINQAVASLPKAG
                     GDLFFLPFLYGSNAGLEMTSGFYGMQAIHTRAHLLQAIYEGVVFSHMTHLNRMRERFT
                     DVHTLRVTGGPAHSDVWMQMLADVSGLRIELPQVEETGCFGAALAARVGTGVYRDFSE
                     AQRDLQHPVRTLLPDMTAHQLYQQKYQRYQHLIAALEGFHARIKEHTL"
     gene            26833..27495
                     /gene="ulaD"
                     /locus_tag="PN977_002215"
     CDS             26833..27495
                     /gene="ulaD"
                     /locus_tag="PN977_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HDI6042244.1"
                     /translation="MSRPLLQLALDHSSLEDAQRDVMLLKGSVDIVEAGTILCLNEGL
                     GAVKALREQCPDKIIVADWKVADAGETLAQQAFGAGANWMTIICAAPLATVEKGHAMA
                     QRCGGEIQIELFGNWTLDDARDWHRLGVRQAIYHRGRDAQASGQQWGEADLARMKALS
                     DIGLELSITGGITPADLPLFKDIRVKAFIAGRALAGAANPAQVAGDFHAQIDAIWGGA
                     RA"
     gene            27488..28348
                     /locus_tag="PN977_002216"
     CDS             27488..28348
                     /locus_tag="PN977_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418039.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HDI6042245.1"
                     /translation="MRNHPLGIYEKALAKDLSWPERLVLAKSCGFDFVEMSVDETDER
                     LSRLDWSAAQRASLVTAMIETGVAIPSMCLSAHRRFPFGSRDEAVRERAREIMSKAIR
                     LARDLGIRTIQLAGYDVYYEDHDEGTQQRFAEGLAWAVEQAAASQVMLAVEIMDTAFM
                     NSISKWKKWDEMLASPWFTVYPDVGNLSAWGNDVPAELKLGIDRIAAIHLKDTQPVTE
                     QSPGQFRDVPFGEGCVDFVGIFKTLHELNYRGSFLIEMWTEKAKEPVLEIIQARRWIE
                     ARMQEAGFIC"
     gene            28342..29037
                     /gene="araD"
                     /locus_tag="PN977_002217"
     CDS             28342..29037
                     /gene="araD"
                     /locus_tag="PN977_002217"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HDI6042246.1"
                     /translation="MLEQLKADVLAANLALPAHHLVTFTWGNVSAVDETRQWMVIKPS
                     GVEYDVMTADDMVVVEIASGKVVEGSKKPSSDTPTHLALYRRYAEIGGIVHTHSRHAT
                     IWSQAGLDLPAWGTTHADYFYGAIPCTRQMTAEEINGEYEYQTGEVIIKTFEERGLNP
                     AQIPAVLVHSHGPFAWGKNAADAVHNAVVLEECAYMGLFSRQLAPLLPAMQNELLDKH
                     YLRKHGANAYYGQ"
     gene            complement(29069..29884)
                     /locus_tag="PN977_002218"
     CDS             complement(29069..29884)
                     /locus_tag="PN977_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDI6042247.1"
                     /translation="MLELSMTLPIKVQNGGLFISRGVGRHPARRLESWEIIFVEKGRL
                     AIQEEERIFLVDAGESLLLWPNRQHVGVEDFPADLKFYWLHFEVKAPDSDPRWLTHLS
                     VPQQTKVADPQVLVALFRQFLSEQEKHQRTPALEFIVLLILQQLTRDARLDEQADGAG
                     TTLAWKAQKLIRTQYHRPLSTALLARELYCNADYLGRVYRRVFHLTLTEAIHRQRVRE
                     AEKLLISEARSLTEVAALCGFNDVGYFRQIFRKHTGLTPAAWKRRYSKEHINS"
     gene            30110..31510
                     /locus_tag="PN977_002219"
     CDS             30110..31510
                     /locus_tag="PN977_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDI6042248.1"
                     /translation="MTSTPITTADIAKGKIDDALSVREKIGYGLGDAGGTVITCLIMN
                     FLTFFYTDVFGLTPALVGTLFIALRVFDAISDPVMGVIADRTQSRWGRFRPWQLWIAI
                     PIGIIGILTFTVPDASMGVKIAWAFGTYLLLSVGYTAINVPYCALINTMTTRHNEVIS
                     CQSWRFVLCGVAGFLVSVGLPWMVDLFGQGNAARGYQLGVGVLCAIAVVMFLCCFFWV
                     RERVPLSTMGKFTLREHLAGLRNNDQLLLMLVMSFLLINVFNIRGGGYMYFITYVLQG
                     STGYTSLFFTMVTFASIIGSVIVSPLTRRFDTVKIYYYTNLLLAALAVLMWFLPSSPA
                     YQTLWLAVILGNGVILGFTLPLHFSLMAFADDYGEWKTRVRSSGMNFAFNLFFIKLAW
                     ASSAGIISLLFIFVAYQPGVENQTASSLGGITAMETLLPALFHLLLAMAIRFCKLNNP
                     MMSRIASDLRQRHVQP"
     gene            31521..>33108
                     /locus_tag="PN977_002220"
     CDS             31521..>33108
                     /locus_tag="PN977_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 127 protein"
                     /protein_id="HDI6042249.1"
                     /translation="MNISEVDLHKLTVSDPFLGQYQQLVRDVVIPYQWDALNDRIPEA
                     EPSHAIENFRIAAGLQDGEFYGMVFQDSDVAKWLEAVAWSLCQKPDAELEKTADEVIE
                     LIASAQCEDGYLNTYFTVKAPEERWSNLAECHELYCAGHLIEAGVAFFQATGKRRLLE
                     VVCRLTDHIDSVFGPDESKLHGYPGHPEIELALMRLYEVTEEPRYLALTNYFVEQRGA
                     QPHYYDQEYEKRGQTSHWHTYGPAWMVKDKAYSQAHLPIAQQQTAIGHAVRFVYLMTG
                     VAHLARLSHDESKRQDCLRLWNNMAQRQLYITGGIGSQSSGEAFSSDYDLPNDTVYAE
                     SCASIGLMMFARRMLEMEGDSQYADVMERALYNTVLGGMALDGKHFFYVNPLEVHPKS
                     LKFNHIYDHVKPIRQRWFGCACCPPNIARVLTSIGHYLYTPREDALYINIYAGNSMEV
                     PVENGTLRLRVSGNYPWQEQVTIAVESPQPVRHTLALRLPDWCTQPQITLNGEEVEQD
                     IRKGYLHITREWQEGDTLNLT"
BASE COUNT         8087 a         8494 c         8347 g         8180 t
ORIGIN      
        1 ctttcggaaa gaccagtgat gaacatggtc gagaatatgc tcgatcaggc attcaaaaag
       61 cttaatcctc acgagcatcc tgttctgcac tctgaccagg gatggcagta tcgtatgaga
      121 agatatcaaa atatccttaa agaacatggt attaaacaaa gcatgtccag aaaaggcaat
      181 tgtctggata atgctgtggt ggagtgtttc tttggaacct taaagtcgga gtgtttttat
      241 cttgatgagt tcagtaatat aagcgagctg aaggatgctg ttacggaata tattgaatac
      301 tacaacagca gaagaattag cctgaaatta aaaggtctga ctccaattga atatcggaat
      361 cagacctata tgcctcgtgt ttaactgtcc aactttctgg ggtcagtaca cactttgatt
      421 tatagtcagg tggggctttt ctctgtctgc ctttcggtga atacctgaga caaacagtct
      481 caagcacccg cagctattct agcttaataa gttcgtttct tctccttgat ataatcctaa
      541 aaaaatctca taaaattaat atatgagaca atctttattc agcagaagat tattaaaggt
      601 tgctctatta tttagcgata aaaaagcctg ccagatggca ggctttttaa taacgacgtt
      661 attactgcaa cagcgaaata tccgcaactc gcaggaataa ttcgcgcagt ttctccagca
      721 tggtaagacg gttgattcgc aattctttgt catcaaccat gaccatcact ttatcgaaga
      781 aggcatcaac cggttcacgc agctcagcca gttcgaccag cgcatcctgg taacgacctt
      841 cagcaaagta cggctctagc ttgtcacgta gcaccacaac ctgcatcgcc agtttaattt
      901 cttccggctc tttcagagta gaggcattca cgcggtcgct cagcacttcg tcagatttcg
      961 ccagaatgtt agatacacgc ttgttcgccg ccgccagtgc tgcagctgca tccagggtac
     1021 ggaagtgcga taccgctttc attcgggcat cgaaatcagc cggacgagtc ggacgacgcg
     1081 ccagtaccgc ctggatggtg tcaacggtgt aaccttcgtc ctgataccag gcgcggaagc
     1141 gaccgagcat aaagtcgata acatcatcaa ccacgttggc attagtcagc ttatcgccat
     1201 acagacgcac tgcttcttcg gtcagggttt gcaggtcaag attgaggttc ttctcaacga
     1261 taatacgcag cacgccaagt gcggcacgac gcaacgcaaa cgggtctttg tcgcctttcg
     1321 gatgctggcc gataccgaag atacccgcca gagtatccat cttgtcagca atcgccagcg
     1381 cacaggctac cgggttagac ggcaggtcat caccggcaaa gcgcggctga tactgctcgt
     1441 tcagcgccac ggcaacatct tccgcttcgc catcgtgacg cgcgtagtgc atccccataa
     1501 cgccctgggt gtcggtgaac tcgaagacca tgttggtcat caggtcgcac ttggacagca
     1561 ggcccgcacg ggttgcgtgg ttaacgtcag cgccaatctg ttcagcaatc cagccagcca
     1621 gcgcctggat gcggtcagtt ttgtcgcgca gtgtacccag ctgttgctgg aacaacacgg
     1681 tttgcaggcg cggcaggtta tcttccagac gttttttacg gtcggtgttg aagaagaact
     1741 cggcatccgc cagacgtgga cgaacgactt tctcgttacc agagataatt tgctgcggat
     1801 ctttcgattc gatattggca acgaagataa agttcggcag cagtttgccg tcgttcgcat
     1861 acaccgggaa gtatttctgg tcacctttca tggtgtaaac cagcgcttca gacggcaccg
     1921 cgaggaattt ctcttcgaat tttgcggtca gcacaaccgg ccattccacc agcgaagcga
     1981 cttcttccaa caggctttcg cttaagtcag cgttaccgcc aatcttacgc gccgcttctt
     2041 cggcatcggc tttaatcttc gccttacgtt cttcgtaatc ggcgatgact ttcccgcgct
     2101 cacgcagaat ttccggatac tgatcggcat tgtcgatagt gaattccgac tcgcccataa
     2161 agcggtgacc gcgaatcacg cgatcggact gaatacccag aatggtagcc ggaataactt
     2221 tgtcgcccag cagcagggtc acggtgtgaa ccggacgcac gaagtgcacg tcgcttgcgc
     2281 cccaacgcat cagtttcggg atcggcagtt tcgccagcga agtcgcaacc atattcggca
     2341 gcagtgcttc ggtgctttcg cccttcacat gggcgcgata cagcagccat tcgcctttat
     2401 cggtagtcag acgctcagcc tggtcaacgg taataccgca accacgcgcc caaccttctg
     2461 ccgctttgct cggtttgcct tcagcgtcga acgcctgggc aattgccggg ccgcgttttt
     2521 cgatttcacg atccggttgc gcttccgcca ggttagccac tttcagcgcc agacgacgcg
     2581 gagcagcaaa ccattgaacg gtgccgtgtg cgaggccagc gttatccagc tccgcagtaa
     2641 agttcgcagc aaaggactca gccaggctgc gcagtgcttt tggtggcagc tcttcagtgc
     2701 cgatttccac cagaaaagtt ttctcagaca tagccgcctc ttacttatct ttgttgcaca
     2761 tcgggaagcc gagggcttca cgggaagcgt agtatgcttc tgccactgct ttggtcaggg
     2821 tgcgaatgcg cagaatatag cgctgacgct cggtgacgga gatggcttta cgcgcatcca
     2881 gcaggttgaa gctgtgggcg gctttcagaa tacgctcgta ggctggcagc ggcagcggat
     2941 tttccagcgc cagcagctgc tgcgcttctt tctcgtactg ctcaaagcag gtgaacagga
     3001 aatccacatc cgcgtattcg aagttgtaag tggactgctc cacttcgttc tgatggaaca
     3061 cgtcgccgta ggtggtttta cccagcgggc cgtcgctcca gaccaggtcg taaacgctgt
     3121 ctacgccctg aatgtacatt gccagacgtt ccagaccgta ggtgatctcg ccggtaaccg
     3181 gtttacactc cagaccacca acctgctgga agtaagtgaa ctgcgtcact tccatgccgt
     3241 tcagccacac ttcccagccc agcccccagg cacccagcgt cgggttttcc cagttatctt
     3301 ccacgaaacg gatgtcgtgg atggtcgggt ccatgcccag ctctttcaga gaaccaaggt
     3361 acagctcctg aatattgtcc ggcgatggct taatgaccac ctggaactga tagtagtgct
     3421 gtaaacggtt ggggttttcg ccgtagcgac catcggtcgg gcgacgagaa ggctgaacat
     3481 aagcagccgc catcggttct ggccccagcg cgcgcagaca ggtcattggg tgagaggttc
     3541 ccgcgccgac ttccatgtcc aatggttgaa caatggtgca gccctggcga gcccagtaat
     3601 cctgtaaggt caggatcaag ccctggaagg tcctggtatc aaacttttgc atattatttc
     3661 gtgctggata cgtgtggatt taaaggaagg gatcagtata cccgctggat ggaagatata
     3721 cagtacgaaa cgggaaaaag cagggcttaa cgcatggaaa gatgcaaaaa atggccatct
     3781 gcgtcaaaag aacagacaaa ctgctcttta cggaaggtat aaccgcgcat ttcgtagctc
     3841 ccctggaact gttcaaatgc ggtgacgtcg attttttgcg cacctgtatt atagcgtttt
     3901 gccgcctgat ctttgcatag cccttccata ttcaacgaac gcagaggatc aacctttacc
     3961 cgttgtgctt tctgcacggg tggcggtgta gaacaaccca ctagcgccat aagcaccata
     4021 agcgccattg ccggaaagaa agagttcatc atcattaaat taccgtctgg tttgcaggtg
     4081 agtcttatta tttatatcgg tatagtggct gatatcttac gaatttcctt aagcctctat
     4141 aagctgtagt gataaaactc agattattcc agtgcgccaa ccgaatgatt tatcggtcag
     4201 acttttttta accagataca gagacacaga tgcagcccaa aatttactgg attgataacc
     4261 tgcgagggat agcgtgttta atggtggtga tgattcacac cactacctgg tatgtgacca
     4321 atgctcatag tgttagcccc gtcacatggg atatcgccaa tgttctgaat tctgcctctc
     4381 gtgtcagcgt gccgctattt ttcatgattt ccggctatct cttttttggc gaacgcagcg
     4441 cccagccgcg ccatttcttg cgcatcggct tatgcctgct gttttatagc actgttgcgc
     4501 tgctctacat tgcgctgttt acctccatca atatggagtt agcgctgaaa aacctgctgc
     4561 aaaagccagt gttttaccac ttgtggtttt tcttcgcgat tgcggtgatt tatctggttt
     4621 caccgctgat tcaggtgaag aacgtcggcg gaaaaatgtt gctggtgcta atggtggtga
     4681 ttggcattat cgctaaccca aacacagtgc cgcagaaaat tgacggtttt gaatggctgc
     4741 caattaactt atatatcaat ggcgatactt tttactacat cctgtatggc atgttgggcc
     4801 gcgctatagg gatgatggac acacagcata aagcactgtc gtgggtgtgc gccgcactgt
     4861 ttgcgacggg ggtatttatt atctctcgcg ggacattata tgaattgcag tggcgcggaa
     4921 attttgccga tacctggtat ctttactgtg ggccgatggt ttttatctgc gcaatcacgc
     4981 tattgactct ggttaaaaac acgctggata cacacactgt tcccgggctt gggctgatat
     5041 cacgccattc tttaggtata tacgggttcc atgcattgat tatccatgcg ctgcgtaccc
     5101 gggggattga gcttaaaaac tggccaatac tcgatattat ttggatcttt tgcgcgacgt
     5161 tggcagcgag tttgttactt tctatgctgg tacaacgaat cgacagaaac agactagtga
     5221 gttaaataaa agcccggtca cattggactg accgggctta tgtgaattat tcgctgtatt
     5281 cttcctgggt aacctgtgtt tcttttggtg ggtttattcc ggcatcacga atattcttct
     5341 gcaccgctac tgcaccaaac aagcttaaga agaaagccag cccataaaag cctttttcgc
     5401 tgagtaataa cgttgcattc cacagaccta ccagcaataa cgcaacagag ataataaaga
     5461 ccgtcaggca agtcatataa taaatggagg tggtcggtat gccttcatac ttgtcccgaa
     5521 cggtcttttg ataagacgcc gcagaaaaca gtcctaatac cagtacggca aaataatatc
     5581 ctttttcatt taactgcatc tctgcattcc atagtcctaa cagataggta acgataccac
     5641 caacgagagc tatccatgac acaatactaa atgccggtga ataggttgat attttgttgt
     5701 ccatcacagt attcctttca ttcctgaatg tgtaagaggt ttatgttgct catgccgagc
     5761 gaaacaaacg catttgacca aacaagcaga caaaaaaggc gatgggatag atcaccattt
     5821 cataagcatt gataggtgcg tttaaaacac cgataaacaa gagaccgata gtgattaacg
     5881 ccagcaactg gcagacgcgg ataaaaaaat catccagttg cccgagtttt tctgcgcgaa
     5941 gatatgcata attgccaaaa gttgctgtca ttaacacgcc aagaaaatag ccttttccac
     6001 ttaacaatgg gcatgagatc catagcccaa ccagataaac cagcgccccg acaacaaata
     6061 atagttttgc cacagtcttt gaggttttca taacgatctc catatctacc agcgatacat
     6121 tacgagtaac caacgaaaga caaaactgaa aaatgccatt aacaaatgat tttcagaata
     6181 aattcatact aaatattaat taattactga gatatataga tgtgaattat cccccacccg
     6241 gtcaggcagg ggataacgtt tacgccatta atggcagaag ttgctgatag aggcgacgga
     6301 acgtttctcg tcgtggctga taagcggcat aacgctgcgc atctggtaga tgcgactgtt
     6361 ctaacggtag ttgtggcaac aattcaatga gcgatttctc tggattcgtc gcaatctgcg
     6421 ccagccttgc tgcacccagt gctggcccca catctccccc tgtgcggtaa tcgagctgct
     6481 gaccgctgat atccgccagc atctgacgcc agtactcact acgagccccg ccaccaatca
     6541 gcgtaacact ttgcggttta atgccgcagg catgtacgac atccatgcca tctgccagcg
     6601 cataacccac gccttccagc actgctcgcg ccagttcatt agcaccatgt tgatgggtca
     6661 aaccaaagaa aacccccttc gcctggggat tgttgtgtgg cgtacgttca ccggaaagat
     6721 aaggcagaaa ccagacaggc tcggtacttt catcagcctg ttgagcagcc gcgattaaag
     6781 ctggaacatt gctcagaccg gttaattttg cggcccaatc caggcaagaa gccgcactca
     6841 gcatcacaga cattaaatgc caacgctgcg ggagcgcatg gcaaaagctg tgtacggcgc
     6901 tttctggctt gcttaagaat ccttcgctga cagcaaaata gacccccgac gtccccagcg
     6961 ataacattgc ctgattagca tcaaccattc ccacaccaac cgcaccagct gcattatcgc
     7021 caccgcctgc gacaactggc accgtcgcca taccccacgc tttcgcaact tcaggtagca
     7081 aagcaccagt aatttcgctg ccttcgtata atgcgggcat ctggtcacga gataagtggc
     7141 aagcctgcag catgagatcg ctccagtcac gctttgcgac atccagccac atagtgccag
     7201 ctgcgtcaga catatcgctg gcaaactccc ccgtcatacg cagacgcaag taatctttcg
     7261 gtaataatac tttatcgatt tgacggaata tctccggctc atgccgatga acccatagca
     7321 atttaggcgc agtaaatccg ggcatcatca ggttaccggt aatcacccgt gattgcggaa
     7381 ctcgcgcttc cagcaaagtg cactcttgcg cacagcgccc gtcgttccac aaaatggcag
     7441 ggcgtaatac ccgttgctgg gcatccagta aggttgcacc gtgcatctgt ccggcaatac
     7501 ctaatgcttt aacgtcttgc agagaatgct gaacgcccag agctttcatt gcgcgatcag
     7561 ttgcctgcca ccactgttcc gggtcttgtt ccgaccagag tggatgcggg cgcgaaacgg
     7621 tcagcttttc cgtttgcgca gcaaccacct caccctgctc attgagcaaa ataactttta
     7681 cgcccgaggt gccaagatct atcccgatat acatatcgat cgttccttaa aaaaatgccc
     7741 ggtatcgcta ctgataaccg ggccaacgga ctgcacagtt agccgttatt tgtcgaacag
     7801 ataatgattt accagatttt ccagttgctc ctggcgacca ctctgatgca ccggagacaa
     7861 attatgttcc tgagcatatt tggctaaatc tgccagtgac atttggcctt tcaggatttg
     7921 ctggcctaat tcgctattcc agccggaata acgctgcgcg atgcgtttat ccagctcgcc
     7981 atcttcaatc atgcgcgctg caattttcag cgccaacgcc atcgtatcca tcgcgccgat
     8041 atgaccgtaa aacagatcat atttatcagt actttgacga cgtactttgg catcgaagtt
     8101 cagaccaccg gtggtgaaac cgcctgcttt gagaatttca tacatcacca gcgcattctc
     8161 ttccacactg ttcgggaact ggtcggtgtc ccagcccagt tgcgcatcgc cacggttggc
     8221 gtcgacagaa ccgaacaggc caagcgcaat ggcggtggct atttcatgat ggaaagagtg
     8281 acctgccagc gtcgcgtggt tagcttcaat attcagttta atctcttttt ccagaccaaa
     8341 ctgtttcagg aagccataga ccgtcgcagc atcgtaatca tattgatgtt tggtcggttc
     8401 ttgcggtttc ggttcgataa gcaacgtgcc ctggaaaccg attttatgtt tatgctcaac
     8461 caccatctgc ataaagcggc ccagctgttc acgctcctgg cgcaagtcgg tatttaacag
     8521 cgtttcgtaa ccttcacgac caccccacag gacatagttt tcaccgccca atttatgggt
     8581 tgcttccatc gctgtaacaa cttgcgttgc cgcccaactg aagacttcag gatctgggtt
     8641 cgtcgccgca cccgcgccat agcgagggtt tgtaaagcag ttagcagttc cccacagcag
     8701 cttcacgccg ctctcttctt gcttgcctgc caggacatca accatttgcg caaaattatt
     8761 gatgtactct tttaacgacg cgccctcagg ggaaacatct acatcgtgga agcaataaaa
     8821 tggcacatgt aacttgtgga aaaactcaaa tgcgacatct gctttacgct tcgccaacgc
     8881 cagtgcctca ccaggctgct gccacggacg attaaacgcc cccacaccaa acatatccgc
     8941 cccgttccag cagaaggtgt gccagtagca ggcggcaaaa cgcaaatgct cttccatacg
     9001 cttgcccaac accagttcgt cgggattgta gtgacggaat gctaacgggt ttgaggattt
     9061 tgagccttca taacgaacgc gatcgagctg gtcaaaatag gcttgcatat tgaactccat
     9121 aatcaggtaa tgccgcgggt gatggatgat gtcgtaatat tgggcactcc ctttcagttg
     9181 ctcaattatg ttatttcaca ctgctattga gataattcac aagtgtgcgc tcgctcgcaa
     9241 aataaaatgg aatgatgaaa ctgggtaatt cctcgaagag aaaaatgcaa taagtacaat
     9301 tgcgcaacaa aagtaagatc tctgtcataa atcaagaaat aaaccaaaaa tcgtaatcga
     9361 aagataaaaa tctgtaattg ttttcccctg tttagttgct aaaaattggt tacgtttatc
     9421 gcggtgattg ttacttatta aagctgtcct ctaactacag aaggccctac accatgaaaa
     9481 taaagaacat tctactcacc ctttgcacct cacttctgct taccaacgtt gcggctcacg
     9541 ccaaagaagt caaaataggt atggcgattg atgatctccg tcttgaacgc tggcaaaaag
     9601 atcgagatat ttttgtgaaa aaggcagaat ctctcggcgc gaaagtattt gtacagtctg
     9661 caaatggcaa tgaagaaaca caaatgtcgc agattgaaaa catgattaac cggggcgtcg
     9721 atgttcttgt tattattccg tataacggtc aggtattaag taacgttgta aaagaagcca
     9781 aacaagaagg tattaaagta ttagcttacg accgtatgat taacgatgcg gatatcgatt
     9841 tttatatttc tttcgataac gaaaaagtcg gcgaactgca ggcaaaagcc ctggtcgata
     9901 ttgttccgca aggtaattac ttcctgatgg gcggctcgcc ggtagataac aacgccaagc
     9961 tgttccgcgc cgggcaaatg aaagtattaa aaccttatgt tgattccgga aaaattaaag
    10021 tcgttggtga ccaatgggtt gatggctggt taccggaaaa cgcattgaaa attatggaaa
    10081 acgcgctaac cgccaataat aacaaaattg atgctgtagt tgcctcaaac gatgccaccg
    10141 caggaggagc aattcaggca ttaagcgcgc aaggtttatc agggaaagta gcaatttccg
    10201 gccaggatgc ggatcttgca ggtattaaac gtattgctgc cggtacgcaa actatgacgg
    10261 tgtataaacc tattacattg ttggcaaata ctgccgcaga aattgccgtt gagttgggca
    10321 atggtcagga gccaaaagcg gataccacac tgaataatgg cctgaaagat gttccctccc
    10381 gcctgctgac accgatcgat gtgaataaaa acaacatcaa agatacggta gttaaagacg
    10441 gattccacaa agagagcgag ctgtaagcgt tacgccccgg cgcggagcgg gggcgtgatt
    10501 tctctccatg ccgcgtgaat taattggctt aggtggagtc gttatgcctt atctacttga
    10561 aatgaaaaat attaccaaaa ccttcggcag tgtgaaggcg attgataacg tcagcttgcg
    10621 gttgaatgct ggcgaaatcg tctcactctg tggggaaaat gggtcaggta aatcaacgct
    10681 gatgaaagtg ctgtgtggta tttatcccca tggttcctac gaaggcgaaa ttatttttgc
    10741 gggagaagag attcaggcga gtcacatccg cgataccgaa cgcaaaggta tcgccattat
    10801 tcaccaggaa ttggcgctgg tgaaagaact gaccgtgctg gaaaatatct tcctgggtaa
    10861 cgaaataacc cataatggca ttatggatta tgacctgatg acgctacgct gtcagaagct
    10921 gctcgcacag gtcagtttat ccatttcacc tgatacccgc gttggcgatt taggacttgg
    10981 gcaacaacaa ctggttgaaa ttgccaaggc acttaataaa caggtgcgtt tgttaattct
    11041 cgatgagccg acagcctcat taactgagca ggaaacgtcg gttttactgg atattattcg
    11101 cgatctacaa cagcacggta tcgcctgtat ttatatttcg cacaaactca acgaagtcaa
    11161 agcgatttcc gatacgattt tcgttattcg cgacggtcag cacattggca cgcgtgatgc
    11221 tgctggaatg agtgaagacg atattatcac catgatggtc gggcgagagt taaccgcgct
    11281 ttaccctaat gaaccacata ccaccggaga tgaaatatta cgtattgaac atctgacggc
    11341 atggcatccg gttaatcgtc atattaaacg agttaatgat gtctcgtttt ccctgaaacg
    11401 tggcgaaata ctgggtatcg ccggactcgt tggtgccgga cgtaccgaga ctattcagtg
    11461 cctgtttggt gtgtggcccg gacaatggga agggaaaatt tatattgacg gcaaacaggt
    11521 agatattcgt aactgtcagc aagccatcgc ccaggggatt gcgatggtac ccgaagacag
    11581 aaagcgcgac ggcatcgttc cggtaatggc ggttggtaaa aatattaccc tcgccgcact
    11641 caataaattt accggcggta ttagccagct tgatgacgcg gcagagcaaa aatgtattct
    11701 ggaatcaatc cagcaactca aagttaaaac gtcgtcctcc gaccttgcta ttggacgttt
    11761 gagcggcggc aatcaacaaa aagcgatcct cgctcgctgt ctgttactta acccgcgcat
    11821 tctcattctt gatgaaccca ccaggggtat cgatattggc gcgaaatacg agatctacaa
    11881 attaattaac caactcgtcc agcagggtat tgccgttatt gtcatctctt ccgaattacc
    11941 tgaagtgctc ggccttagcg atcgtgtact ggtgatgcat gaagggaaac taaaagccaa
    12001 cctgataaat cataacctga ctcaggagca ggtgatggaa gccgcattga ggagcgaaca
    12061 tcatgtcgaa aagcaatccg tctgaagtga aattagccgt accgacatcc ggtggctttt
    12121 ccgggctgaa atcactgaat ttgcaggtct tcgtgatgat tgcagctatc atcgcaatca
    12181 tgctgttctt tacctggacc accgatggtg cctacttaag cgcccgtaac gtctccaacc
    12241 tgttacgcca aaccgcgatt accggcatcc tcgcggtagg aatggtgttc gtcataattt
    12301 ctgctgaaat cgacctttcc gtcggctcaa tgatggggct gttaggtggc gtcgcggcga
    12361 tttgtgacgt ctggctaggc tggcctttgc cacttaccat cattgtgact ttggttctgg
    12421 gactgcttct cggtgcctgg aacggatggt gggtcgcgta ccgcaaagtc ccttcattta
    12481 ttgtcaccct cgcgggcatg ttggcatttc gcggcatact cattggcatc accaacggca
    12541 cgactgtttc ccccaccagc gccgcgatgt cacaaatcgg gcaaagctat ctacccgcca
    12601 gcaccggctt catcattggc gcgcttggct taatggcttt tgttggctgg caatggcgcg
    12661 gaagaatgcg ccgtcaggct ttgggtttgc agtctccggc ttctaccgca gtagtcggtc
    12721 gccaggcttt aaccgctatc atcgtattag gcgcaatctg gctgttgaat gattaccgtg
    12781 gcgttcccac tcctgttctg ctgctgacgt tgctgttact cggcggaatg tttatggcaa
    12841 cgcggacggc atttggacga cgcatttatg ccatcggcgg caatctggaa gcagcacggc
    12901 tctccgggat taacgttgaa cgcaccaaac ttgccgtgtt cgctattaac ggattaatgg
    12961 tagccatcgc cggattaatc ctcagttctc gacttggcgc tggttcacct tctgcgggaa
    13021 atatcgccga actggacgca attgccgcat gtgtgattgg cggcaccagt ctggcaggcg
    13081 gcgtcgggag cgtggccgga gcggtaatgg gggcattcat catggcttca ctggataacg
    13141 gcatgagtat gatggatgta ccgaccttct ggcagtatat cgttaaaggt gcgattctgt
    13201 tgctggcagt atggatggac tccgcaacca aacgccgttc ttgattttga taaaaatttt
    13261 ctcaaagccg gtaacgtatt accggttttg agtttttgca tgattcagca ggaaaagaac
    13321 catgtttact aaacgtcacc gcatcacatt actgttcaat gccaataaag cctatgaccg
    13381 gcaggtagta gaaggcgtag gggaatattt acaggcgtca caatcggaat gggatatttt
    13441 cattgaagaa gatttccgcg cccgcattga taaaatcaag gactggttag gagatggcgt
    13501 cattgccgac ttcgacgaca aacaaatcga gcaagcgctg gctgatgtcg acgtccccat
    13561 tgttggggtt ggtggttcgt atcaccttgc cgaaagttac ccacccgttc attacattgc
    13621 caccgataac tatgcgctgg ttgaaagcgc atttttgcat ttaaaagaga aaggcgttaa
    13681 ccgctttgct ttttatggtc ttccggaatc aagcggcaaa cgttgggcca ctgaacgcga
    13741 atatgcattt cgtcagcttg tcgccgaaga aaagtatcgc ggagtggttt atcaggggtt
    13801 agaaaccgcg ccagagaact ggcaacacgc gcaaaatcgg ctggcagact ggctacaaac
    13861 gctgccaccg caaaccggga ttattgccgt tactgacgcc cgggcacggc atattctgca
    13921 agtatgtgaa catctacaca ttcccgtacc ggaaaaatta tgcgtgattg gcatcgataa
    13981 cgaagaactg acccgctatc tgtcgcgtgt cgccctttct tcggtcgctc agggcgcacg
    14041 gcaaatgggc tatcaggcgg caaaactgtt gcatcgatta ttagataaag aagaaatgcc
    14101 gctacagcgg attttggtcc caccagttcg cgtcattgaa cggcgctcaa cagattaccg
    14161 ctcgctgacc gatcccgccg ttattcaggc catgcattac attcgtaatc acgcctgtaa
    14221 agggattaaa gtggatcagg tactggatgc ggtgggcata tcacgctcca atcttgagaa
    14281 gcgttttaaa gaagaggtgg gtgaaaccat ccatgcaatg attcatgccg agaagttgga
    14341 gaaagcgcgc agtctgctga tttcaaccac cttgtcgatc aatgagatat cgcaaatgtg
    14401 cggttatcca tcgctgcaat atttctactc tgtttttaaa aaagcatatg acacgacgcc
    14461 aaaagagtat cgcgatgtaa atagcgaggt catgttgtag cgatgatgag aattgtcggc
    14521 gtcgcatcag gtaactcagc acaaccaccc gatacgagca aaagccttat ccgcctgaca
    14581 agtcggataa ggctcgagga aatgcaatta catatgcgcc gcgattaacc gttggttatc
    14641 ctggtacatt gcgaacaggt agttgttata actcttccct ttggtcgagt agcccttcag
    14701 cttgtgaatc atcgctgtgg cagtcacttc ctgatccgct ttacgcagtt gtgcacgcga
    14761 tttacggaac gaagagtaag ccgggtgcgt attgaggtta gtgacatagg cgctcaccga
    14821 ttctttgaca gaactaaact gtgagtaccc tttcacttta cccggcgcat tggtacaacg
    14881 tcctttcatg catttcatgc cgaacaggtt gttgttgttg cgcgccagct tcgacgttcc
    14941 ccaaccgctt tctgctgcag ccatcgtcgc caccatactg gtagggataa tgtctacgcg
    15001 ttcaaggaaa gtattccacg ggatttttcg cgtattacca gaccacttca ccttgtagcg
    15061 tttggcgatg tctttcagac gcgcacgctc agcaggtgac cattggccct gatactgttt
    15121 tgaaatcagc cagttacgtt ccgcagtaat ggccgcattt tggctggtaa tgtaaggcat
    15181 tacggtccgg agaaacgcct ttttccttgg tgttccggaa gggtattttc gcaaatcagg
    15241 aagtgaacta ctctttgcac tattgcgaga atactcttgt ttactgctta cctgtttatt
    15301 actagcttta gttaagtggg acttttgact cgctgttgtt gtgtgcgtct tcgctaacac
    15361 ctcactcgaa aacaccagag tgagtaacat aagaatcatc gccccatatc gtcgtatggg
    15421 agtcaaaatc atcaggtctc ctggtcggat ttaatcattc caacacctta tatttttcac
    15481 aaatttgaga gttgaatctc aaatcatatc aaaaatagct gtcaagagca ccccaaggaa
    15541 tagtccaaat ctgaaactat gtcacgagtt aacgattcag attggcgcta aatcgcagaa
    15601 aatgtggggt ttatcgcaaa attcagccgt tttttgcgcg cgatcgctca cccttgcttc
    15661 tcatcctgtg gacttaccgc tcagggatga gttttgtctg gcttatcgct ggcaaactgt
    15721 ctgaaatcgc agcaataagg actcatccgc catgaaactc gccgcctgtt ttctgacact
    15781 ccttcctggc ttcgccgttg ccgccagctg gacttctccg gggtttcccg cctttagcga
    15841 acaggggacg ggaacatttg tcagccacgc acagttgccc aaaggtacgc gtccactaac
    15901 gctaaatttt gaccaacagt gctggcagcc tgcggatgcg ataaaactca atcagatgct
    15961 ttccctgcaa ccttgtagca acacgccgcc tcaatggcga ttgttcaggg acggcgaata
    16021 tacgctgcaa ctagacaccc gctccggtac accaacattg atgatttcca tccagaacgc
    16081 cgtcgaaccg gtagcaagcc tggtccgtga atgcccgaaa tgggatggat taccgctcac
    16141 actggatgtc agcgccactt ttccggaggg agccgccgtc cgggattatt acagccagca
    16201 aattgcgata gtgaagaacg gtcaaataac gttacaaccc gctgccacca gcaacggttt
    16261 actcctgctg gaacgggcag aaactgacgc ctctgcccct ttcgactggc ataacgccac
    16321 ggtttacttt gtgctgacag atcgtttcga aaacggcgat cccagtaatg accagagtta
    16381 cggacgtcat aaagacggta tggcagaaat tggcactttt cacggcggcg atttacgcgg
    16441 cctgaccaac aaactggatt acctccagca gctgggcgtc aatgctttat ggataagtgc
    16501 cccatttgag caaattcacg gctgggtcgg cggcggtaca aaaggcgatt tcccgcatta
    16561 tgcctaccac ggttattaca cacaggactg gacgaatctt gatgccaata tgggccgcga
    16621 agccgatcta cggacgctgg ttgatagcgc gcatcagcgc ggtattcgta ttctctttga
    16681 tgtcgtgatg aaccacaccg gttacgccac actggcggat atgcaggagt ttcagtttgg
    16741 cgcgttatat ctttctggtg acgaagtgaa aaaaacgctg ggtgaacgct ggagcgactg
    16801 gaaacctgcc gccgggcaaa cctggcatag ctttaacgat tacattaatt tcagcgacaa
    16861 aacaggctgg gataaatggt ggggaaaaaa ctggatcaga accgatatcg gcgattacga
    16921 caatcctgga ttcgacgatc tcactatgtc gctggccttt ttgccggata tcaaaaccga
    16981 atcaacgacc gcttctggtc tgccggtgtt ctataaaaac aaaacggata cccacgctaa
    17041 agtcatcgac ggctttaccc ctcgcgatta cttaacccac tggttaagtc agtgggttcg
    17101 cgactatggg attgatggtt ttcgggtcga caccgccaaa catgttgagt tgcccgcctg
    17161 gcagcaactg aaaaccgaag ccagcgccgc gcttcgcgaa tggaaaaaag ctaactccga
    17221 caaagcatta gatgacaaac ctttctggat gaccggtgaa gcctggggcc acggcgtgat
    17281 gcaaagtgac tactatcgcc acggcttcga tgcgatgatc aatttcgatt atcaggagca
    17341 ggcggcgaaa gcagtcgatt gtctggcgca gatggatacg acctggcagc aaatggcgga
    17401 gaaattgcag gatttcaacg tgttgagcta cctctcatcg catgataccc gcctgttccg
    17461 cgaaggtggt aataaagcag cagagttatt gttattagcg ccaggcgcgg tacaaatctt
    17521 ttatggtgat gaatcctcgc gtccgttcgg ccccaccggt tcggatccgc tgcaaggtac
    17581 gcgttcggat atgaactggc aggatgttag cggtaaatct gccgccagcg tcgcgcactg
    17641 gcagaaaatc agccagttcc gcgcccgcca tcccgcaatt ggcgagggca aacaaacgac
    17701 actttcgatg aagcagggct acggctttgt tcgtgagcat ggcgacgata aagtgctggt
    17761 catctgggct gggcaacagt gacttttccg gcttcccgtt cgtcagtacc tcgggaagcc
    17821 gccaaccagg ataaaatgtc agctctaatc agcgttgcag gataaagcac cgctccctct
    17881 tcaacagagc gatttgcacc ccagcaaatg tagcgttatt gttaccttcc ttgctacaga
    17941 gttcgacaga tatcccgcta tgacattctc cctttttggt gacaaattta cccgccactc
    18001 cggcattacg ctgctgatgg aagatctgaa cgacggttta cgcacgcctg gcgcgattat
    18061 gctcggcggc ggtaatccag cgcagatccc ggaaatgcag gactacttcc agacgctact
    18121 gaccgacatg ctggaaagtg gcaaagcgac tgatgcactg tgcaattacg acggtccaca
    18181 ggggaaaacg gagctactca cactgcttgc cggaatgctg cgcgagaagt tgggttggga
    18241 tatcgaacca cagaatattg cactaacaaa cggcagccag agcgcgtttt tctacttatt
    18301 taatctgttt gccggacgcc gtgccgatgg tcggatcaaa aaagtgctgt ttccgctggc
    18361 accggaatat attggctacg ctgacgccgg actggaagaa gatctgtttg tctctgcgcg
    18421 tccgaatatt gaactgctgc cggaaggcca gtttaaatac cacgtcgatt ttgagcatct
    18481 gcatattggc gaagaaaccg ggatgatttg cgtctcccgg ccgacgaatc caacaggcaa
    18541 tgtgattact gacgaagagt tgctgaagct tgacgcgctg gcgaatcaac acggcattcc
    18601 gctggtgatt gataacgctt atggcgtccc gttcccgggt atcatcttca gcgaagcgcg
    18661 cccgctgtgg aatccgaata ttgtactgtg catgagtctt tccaagctgg gactaccggg
    18721 ttcgcgctgc ggcattatca tcgccaatga aaaaatcatc accgcaatca ccaacatgaa
    18781 cggcattatc agcctggcgc ctggcggtat tggtccggcg ataatgtgtg aaatgattaa
    18841 gcgtaacgat ctgctgcgcc tgtctgaaac ggttatcaaa ccgttttatt accagcgtgt
    18901 tcaggaaact atcgccatca ttcgccgcta tttaccggaa gatcgctgcc tgattcataa
    18961 accggaagga gccattttcc tctggctatg gtttaaggat ttgccaatta cgaccgagca
    19021 gctctaccag cgcctgaaag cacggggcgt gctgatggtg ccggggcaca acttcttccc
    19081 agggctggat aaaccgtggc cacatacaca tcaatgtatg cgcatgaact acgtgccgga
    19141 gccggataaa attgaggcgg gggtgaagat tctggcggaa gagattgaac gcgcctgggc
    19201 tgaaagtcat taaaaattgc ctgatgcgct acgcttatca ggcctacatc atttctgcaa
    19261 tatattgaat ttggaagaat ttgtaggccg gataaggcgt ttaagccgca tccggcataa
    19321 acaaagcgca ctattcctgc gttaaaaaac gccggaaaaa cgtttcaaac agactgctgt
    19381 cgtagccgat gccgctgcac tttctcgaca tccatgcact gcaacgcctt tgtcgggcag
    19441 gcttcaacac aggccggtcc cgtcgcccga tgccagcaca gatcgcactt aatcgccctc
    19501 gcctttttgc gcgaagaaac gacctccatc gcaccaaacg ggcaagccag catacagctt
    19561 ttacagccaa tgcaacgtga ttgttcaacg aaaatatgcc catgttcacg gcttatcgcg
    19621 tcaacagggc agacattcgc gcacggtgca tcttcacact gatgacaggc tactgccgtg
    19681 gtccagctgt ggtctttaat gacacgaata cgggaaataa actcgtctgg tgacaacgca
    19741 gcgcaatcct gattctcctg atgcgacact gcgcaagcca cttcacaggt acggcaaccg
    19801 atacatttcg tcgcatccgc aataataaac cggttcatga tcactccttt gcactaaaac
    19861 agcagagtgc cagagagcgc gtctctactg ctttgatcca acgaaggaaa tgttaaagtt
    19921 ttggcacttg tccaaagcgt tatgtgatcg cgccctggtc gtccctgaca gtaaatccca
    19981 gttcattaga aatagcctgc gcggtttcac gcagtggttt caggagattt ttctccccca
    20041 cctgtttcag acgtgatgtc gaaagcgaaa tcgacacggc gtacggcacc cgcccatgaa
    20101 tatcaaacac cggaacagca atacaggaga cgccgagttc gttttcttct ctgtccatcg
    20161 ccgctccgct ttcacgaatg tgcgccagtt cgtcgaacat cgcgggcagc tcggtaatgg
    20221 tattgcgggt taacggctgg atctcatgct gatggctttc ccagtatgac ttcacgtagt
    20281 ccgggtgacc aaacgccatg tagatcttgc ccattgcgga acagtagagc ggcatatgct
    20341 ggcctatata ggcacgggtt cgcagcatcc cggttgtggg ttccagctta taaatcaaaa
    20401 tagcgtgatc gtcttcgcgg ctggagaagt taatggtttc accagtggcg atgttcaatg
    20461 cctcaagatg cggagcggcg atatgaatga tattcagcga agacagcgcc ttctgcccga
    20521 cggcaataaa tttggtggtc aggcggtaac tccctgcggc gggcgcggtg gtcacatagc
    20581 cgcaggactg taatccctgc aataagcgat ggacggtact cttatttaaa ccagccagtt
    20641 ccgaaagatg cgccaacgga caaccgtttg gatagttgct caaaatctca atcagcatca
    20701 acccacgaaa cagactctgg cttccggctg gacgctcttt ttcctgcgcc atctcgttct
    20761 ctttttttcc catcacttct ttccccattt tgtcgcgtcc tgatggtagc gcaaagtgtg
    20821 ccgtagttca cgatctcgac agataattta taaccacttg atttttaagt tttttgaaat
    20881 tcatcaatca gattgctttg ttaaaaagtg atcgatatat ttgaaatcaa gtttcgtata
    20941 ttgaaatttt aagccaaaaa agcgttcaaa aaaacaagga agcctgggat gaaagtgaca
    21001 tttgagcagt taaaagcagc ctttaatcgg gtcttaattt cacgcggcgt tgacagcgaa
    21061 acggctgacg cctgtgcaga gatgttcgcc cgcaccaccg aatccggcgt ttattctcac
    21121 ggcgttaatc gtttccctcg tttcattcaa caactggaaa acggcgatat cattcctgat
    21181 gcccaaccca aacgtataac cagcctcggc gcaattgaac agtgggacgc ccagcgttcg
    21241 atcggtaacc tgacagcgaa aaagatgatg gatcgcgcca ttgaactggc tgccgatcac
    21301 ggtattggtc tggtggcact acgtaatgcc aaccactgga tgcgcggcgg cagctacggc
    21361 tggcaggcgg cggaaaaagg ctatattggc atttgctgga ccaactccat cgccgtaatg
    21421 ccgccgtggg gcgcaaaaga gtgtcgcatc ggcaccaacc cgctgatcgt cgccattcct
    21481 tctaccccaa tcaccatggt cgatatgtcg atgtcgatgt tctcttacgg catgttagaa
    21541 gttaaccgtc tggcaggccg tcagctcccg gtcgatggtg gctttgatga tgagggcaat
    21601 ttgaccaaag aacctggcgt tatcgagaag aatcgccgca ttttgccgat gggctactgg
    21661 aaaggttctg gcatgtcgat tgtgctggat atgatcgcca ctctcctttc cgacggcgca
    21721 tcggttgccg aagtcaccca ggacaacagc gacgaatacg gcgtttcgca aatctttatc
    21781 gccatcgaag tggataaatt gatcgacggc cccacccgcg atgccaagct gcaacgcatc
    21841 atggattacg ttactaccgc tgaacgcgct gacgaaaacc aggccatccg cttacccggc
    21901 cacgaattta ctaccctgct ggccgaaaac cgccgtaacg gcatcaccgt tgatgacagc
    21961 gtgtgggcaa aaatccaggc gttataagga gataagccat gatttttgga catatcgcgc
    22021 agcccaatcc gtgccgtttg cccgccgcca ttgaaaaggc gctcgatttt ctgcgcacca
    22081 tcgacttcaa cgccctggaa ccgggcgttg tcgaaatcga cggcaagaat atttatgcgc
    22141 aaattatcga tttaaccaca cgcgaagcgg tggaaaaccg tccggaagtg catcgtcggt
    22201 atatcgatat tcagtttctg gcgtggggtg aagagaaaat cggcattgct attgatacgg
    22261 gaaataataa agtcagcgaa tcactattag agcagcgcga tattattttt tatcacgata
    22321 gtgaacatga atcatttata gaaatgatac cgggcagcta cgctattttc ttcccgcagg
    22381 atgttcatcg accaggttgt attctgcaaa cagcctctga gatacgaaaa attgtggtta
    22441 aagtcgcatt aacagcgctg aattaatcga ataatcgtct acgccagaac gcctgggtaa
    22501 tgttattgct ctaactatat taataattta ggttgctgca catgcaactt taattatgac
    22561 agacatagcc tcaaggaata gctatgaaaa aaatacttga agcaatactg gcgattaatc
    22621 tcgccgtact ttcctgtatt gtgtttataa acattatttt aagatatggt tttcagacaa
    22681 gcattttatc tgtagatgaa ttgtcacgtt atttatttgt ctggttaaca tttattggcg
    22741 cgattgtggc ttttatggat aacgcccacg ttcaggtcac ctttctggtg gagaaactct
    22801 cccccgcatg gcagcgacga gtcgctctgg ttacccattc cttaatctta tttatttgtg
    22861 gcgcactggc ctggggcgca acgctaaaaa caattcagga ctggagcgat tattcaccga
    22921 tcctcggttt gcccatcggc ctgatgtatg ccgcttgtct gcccaccagc ctcgttatcg
    22981 cattctttga actgcgtcat ttatatcaac tcatcacgcg tagcaattct ctaacgtcgc
    23041 caccgcaagg agcttaatca tggctgtgct gatttttctg ggctgtctgt tgggtggtat
    23101 tgctatcggt ttgcctattg cctgggcact gttgttgtgc ggggcggcgt taatgttctg
    23161 gctgggcatg tttgatgtcc agatcatggc acaaacgctg gtgaacggag ccgacagctt
    23221 ctccctgctg gcgattccgt tctttgtgct ggcgggtgaa atcatgaatg cgggcggctt
    23281 gtcaaaacgc attgttgacc tgccgatgaa actggtgggg cataaaccag gcgggctggg
    23341 ctacgtcggc gtgctggcgg ctatgattat ggcaagcctt tctggttctg ccgttgcgga
    23401 taccgccgcc gttgccgctc tactggtgcc gatgatgcgc agcgccaact acccggtcaa
    23461 ccgggcggcg gggctgattg cctctggcgg cattatcgcg ccaattattc ccccttccat
    23521 tccgtttatt attttcggcg tttccagcgg attatccatc agtaagctgt ttatggcggg
    23581 cattgcccct ggcatgatga tgggcgcaac gctgatgctt acctggtggt ggcaggccag
    23641 tcgccttaat ttaccccgcc agcaaaaagc aacgatgcag gaaatctggc actcttttgt
    23701 ctccggtatc tgggcgctgt ttcttccagt catcattatt ggcgggtttc gctccggtct
    23761 gtttacccct accgaagccg gggccgttgc tgctttctat gcgctgtttg tcgccacagt
    23821 tatttaccgt gaaatgacct tcgccacact ttggcatgta ctcattggcg cggcgaaaac
    23881 cacctcagtg gtaatgtttc tggtggcctc ggcacaagtt tctgcctggc tgattaccat
    23941 tgctgaattg ccgatgatgg tttctgattt actgcaaccg ttggtcgatt caccgcgtct
    24001 gctgtttatc gttattatgc tggctatcct gattgtcggc atggtcatgg atttaacgcc
    24061 gacggtgtta attcttactc cggtattaat gcctttagtt aaagaggcag gaatcgatcc
    24121 gatttatttc ggtgtcatgt ttatcattaa ctgctcaatc ggcttaatta caccgcctat
    24181 cggtaatgtg cttaacgtta tttcaggggt ggcaaaactc aaattcgatg atgcagtcag
    24241 aggcgttttc ccttacgtcc tggttttata ttcattatta gtcgtatttg tttttattcc
    24301 cgatctcatc atcctccctt tgaaatggat taattaaaag gaaaatatta tgaaattacg
    24361 ctctgtaacc tacgcattat tcattgctgg cctggctgca tttagtacat cttcaatggc
    24421 ggcgcaatct ttacgtttcg gttatgaaac ctcacaaacc gactcgcaac atattgcggc
    24481 gaaaaaattc aatgatttat tgcaggagag aaccaaaggc gagctgaaat taaaactgtt
    24541 cccggacagc actctcggta acgcgcaggc gatgatcagc ggcgtacgtg gcggcaccat
    24601 cgatatggaa atgtccggct cgaataactt tgccgggtta tcaccagtga tgaacttgct
    24661 tgatgtccct ttcctgttcc gcgataccgc tcacgcgcat aaaacgctcg acggcaaagt
    24721 cggtgatgat ctgaaagcct cacttgaagg taaaggactg aaagtactgg cctactggga
    24781 aaacggctgg cgcgatgtca ccaactcgcg cgcaccggtt aaaacccccg ccgacctgaa
    24841 agggctgaaa atccgcacca acaatagtcc gatgaatatc gcagcattca aagtctttgg
    24901 cgctaacccg atcccgatgc cgtttgccga agtctatacc gggctggaaa cgcgcactat
    24961 cgacgctcag gaacacccga tcaacgtcgt ctggtcagca aaattttatg aagtacagaa
    25021 gtacctttct ctgacgcacc acgcctattc gccacttctg gtggtgatca acaaagcgaa
    25081 gtttgacggc ttaaccccgg agttccagca agcgctaatt tcatctgcgc aggaagcggg
    25141 taactaccag cgcaaactgg ttgccgaaga tcagcaaaaa atcatcgacg gcatgaaaga
    25201 agcgggcgtg gaagtcatca ccgatctcga ccgcaaagcc tttagcgacg cactggggaa
    25261 tcaggttcgc gacatgtttg ttaaagatgt gccgcaggga gctgatctgc tgaaagccgt
    25321 ggatgaggtg caataaacca tgacgcaata ctggctgggg ttagattgtg gcggtagctg
    25381 gctgaaagcc gggctgtatg accgcgaagg ccgggaggca ggcgtgcagc gcctgccgct
    25441 gtgcgcatta agcccgcagc caggctgggc agagcgcgat atggcagaac tgtggcaatg
    25501 ctgcatggct gtcattcgca ccctgcttac tcattctggt gtcagcgggg agcaaatagt
    25561 gggtatcggt atctccgcac agggaaaggg cttgtttttg ctggataaaa acgacaagcc
    25621 gctcgggaat gctattttgt cctcggaccg ccgggcgatg gaaatcgttc gtcgctggca
    25681 ggaagatggc atcccggaaa aactctaccc gctgacccga caaaccttgt ggaccgggca
    25741 tccggtgtcg ctgttacgct ggctgaaaga gcacgaaccg gaacgctacg cgcaaattgg
    25801 ctgcgtaatg atgacgcacg actatctgcg ctggtgttta accggcgtca aaggttgcga
    25861 agagagcaat atttccgagt ccaacctcta caacatgagt cttgaggaat atgacccgtg
    25921 cctcaccgac tggctgggga tcgctgaaat caaccacgcc ctgccgcctg ttgtcggatc
    25981 tgccgaaatt tgcggggaaa tcaccgctca gacagccgca ctgaccggtc tgaaagcggg
    26041 tacgcccgtt gtcggcggcc tgtttgatgt ggtttccacc gcactctgtg ccgggatcga
    26101 agacgaattt accctcaatg cggtgatggg cacctgggcg gtgaccagcg gcataacccg
    26161 tggtttacgt gacggtgaag cgcatccgta tgtctatggt cgctacgtta acgatggtca
    26221 atttatcgtt cacgaagcca gccccacctc ttccggcaac ctcgaatggt ttaccgcaca
    26281 gtggggagaa atctcgtttg atgagattaa ccaggccgtt gccagcttgc cgaaggccgg
    26341 gggcgatctc tttttcctgc cgttcctgta cggcagcaac gccggattgg agatgaccag
    26401 cggtttctac gggatgcagg ccattcatac ccgcgcacac ctgttgcagg ccatttatga
    26461 aggcgtggtg ttcagccata tgacccatct caaccggatg cgcgaacgtt ttactgatgt
    26521 gcacaccctg cgcgtcactg gcggcccggc gcattccgat gtctggatgc aaatgctggc
    26581 ggacgtcagc ggtctgcgta tcgagctgcc gcaggtggaa gaaaccggct gctttggtgc
    26641 ggcacttgcc gcccgcgtcg gcaccggagt ttatcgcgat ttcagcgaag cccaacgtga
    26701 tttacagcac ccggtacgca ccctgctgcc ggatatgacc gcacatcagc tttaccaaca
    26761 aaaataccaa cgctatcagc atctcattgc cgcacttgag ggctttcacg cccgcatcaa
    26821 ggagcacaca ttatgagccg accacttctg caactggccc tcgaccactc atcacttgaa
    26881 gacgcacagc gcgacgtgat gctgttaaaa ggcagcgtcg atatcgtcga agcgggcacc
    26941 attctctgtt taaacgaagg gcttggcgcg gtgaaagcct tgcgcgaaca gtgcccggat
    27001 aaaatcatcg ttgctgactg gaaggtcgcc gacgcgggtg aaacgctcgc acaacaggcg
    27061 tttggcgcgg gtgccaactg gatgaccatc atctgcgctg cgccgctcgc gacggtggaa
    27121 aaaggccacg ccatggcgca acgctgcggt ggtgaaattc agatagagct gttcggtaac
    27181 tggacgctgg acgacgcccg cgactggcat cgtcttggcg tgcggcaggc catttatcat
    27241 cgcggtcgcg acgcgcaggc cagcgggcaa cagtggggcg aggccgatct ggcacgcatg
    27301 aaggcgcttt ccgatatcgg ccttgagctt tccattaccg gcgggattac ccctgctgac
    27361 ctgccgctgt ttaaagatat ccgcgtgaaa gcctttattg ccgggcgggc gctggcaggc
    27421 gcagcaaatc cggcgcaggt ggctggcgat ttccatgcac aaattgatgc catctgggga
    27481 ggtgcgcgtg cgtaatcatc cgttagggat ttatgaaaaa gcgctggcga aagatctctc
    27541 ctggccggag cggctggtgc tggcaaaaag ctgtggcttt gattttgtcg aaatgtcggt
    27601 ggatgaaacc gacgaacggc tctcacgtct tgactggagc gccgcacaac gtgcttcgct
    27661 ggtcactgcg atgatcgaaa cgggtgttgc cattccgtca atgtgcctgt ctgcccatcg
    27721 tcgttttccc tttggcagcc gggacgaggc agtgcgcgaa cgggcgcggg aaatcatgag
    27781 taaagcgatt cgcctggcgc gcgatctcgg cattcgcacc atccagctgg caggttatga
    27841 cgtctattac gaagaccacg acgaaggcac ccaacaacgt tttgctgaag ggctggcgtg
    27901 ggcagtcgaa caggcggcgg catcacaagt aatgctggcg gtggagatta tggataccgc
    27961 gtttatgaac tccatcagca aatggaaaaa atgggacgag atgctcgcct cgccgtggtt
    28021 caccgtctac ccggacgtcg gcaacctcag cgcctggggc aatgatgttc ccgccgaact
    28081 gaaactgggc attgaccgta tcgccgcgat ccacctgaaa gacacccagc cagtgaccga
    28141 gcaaagcccc ggacagttcc gtgatgtgcc gtttggcgaa ggctgcgtcg atttcgttgg
    28201 catcttcaaa acgctgcatg aactgaacta tcgcggatca tttctgattg agatgtggac
    28261 cgaaaaagcc aaagagccgg tgctggagat tattcaggcg cggcgctgga ttgaagcgcg
    28321 tatgcaggag gccggattta tatgttagag caactaaaag ccgacgtgct ggcggcgaat
    28381 ctggcgcttc ccgctcacca tctggtgacg ttcacctggg gcaatgtcag cgcggtagac
    28441 gaaacgcggc aatggatggt aatcaaacct tccggcgtcg agtacgacgt gatgaccgcc
    28501 gacgatatgg tggtggttga gatagccagc ggtaaagtgg tggaaggcag caaaaagccc
    28561 tcgtccgaca cgccaacgca tctggcgctt taccgtcgct atgccgaaat tggcggtatt
    28621 gtgcataccc actcgcgcca cgccaccatc tggtcgcagg ccgggctgga tctccccgcc
    28681 tggggcacca cgcacgctga ttacttctat ggtgccatcc cctgcacgcg acagatgacc
    28741 gcagaggaga tcaacggcga gtatgaatac cagaccggcg aagtgatcat taaaaccttc
    28801 gaagaacgtg ggttgaaccc ggcacaaatc ccggctgtac tggtgcattc tcacggcccg
    28861 ttcgcctggg gaaaaaacgc cgccgatgcg gtgcataacg ccgtggtgct ggaagagtgc
    28921 gcctatatgg gcctgttctc gcgccagctt gcaccgctgc tccctgcgat gcaaaacgaa
    28981 ctgctggata agcactacct gcgtaaacat ggggccaatg cctattacgg gcagtaatct
    29041 ctcagaccgg ggcttcatcg ccccggcact acgaattgat atgttccttg ctgtaacgcc
    29101 gcttccacgc tgctggcgtt aaaccggtgt gcttgcgaaa aatctgccgg aaatagccga
    29161 cgtcattaaa cccgcacaac gccgccactt ccgtgagcga tcgcgcttcg ctaattaaca
    29221 gcttttcggc ttcgcgcact cgctggcggt ggattgcttc tgttaacgtc agatgaaaca
    29281 cccgccgata gacgcgccca agataatcgg cattgcagta cagctcgcgg gctaaaagcg
    29341 ccgtggaaag cgggcggtga tattgcgtgc gaatcagttt ctgcgctttc caggcaaggg
    29401 tggtgcctgc accatcggcc tgttcatcaa gacgcgcgtc acgggtgagc tgctgcaaaa
    29461 tcagcagcac aataaactcc agcgccggag tgcgttgatg cttttcctgt tcgcttaaga
    29521 actggcgaaa caaggccacc agcacctgcg gatcggcaac cttagtttgt tgcggcacgc
    29581 taagatgagt gagccagcgc gggtcgctat ccggcgcttt aacctcaaaa tgcagccagt
    29641 aaaatttgag atcggcggga aaatcctcta ccccaacatg ctgtcggttc ggccagagta
    29701 ataaactctc tcccgcatcg accagaaata tccgctcttc ttcctgaatc gctaatcgac
    29761 ctttttcgac aaagataatt tcccatgact ccagccgcct tgccggatgc ctccctacgc
    29821 cacgggaaat aaataatcca ccattttgca ctttaatcgg taacgtcatg gataattcga
    29881 gcattgatat gtaattttcc gcttattcat taatgattaa acacattacc ttttgattat
    29941 taatcaaaaa cattaatttc acctgatcca attcacactt ttaaattcag gtcggaatcg
    30001 tcaccttttt ttactctttt ctcgccttgt tgatattccc gttcacggca gaattaatca
    30061 tgtcattgca ataaaactaa aaattgctcc ctattcacga ggaatatcta tgacctctac
    30121 accgattact accgctgata tcgctaaagg taaaattgac gatgcgttat ctgtacggga
    30181 aaaaataggc tacggcctgg gtgacgcagg cggcaccgta ataacttgcc tgatcatgaa
    30241 ctttctcacc tttttctaca ccgacgtttt tggcttaact ccggcgctgg tcggcacgct
    30301 gtttattgca ctgcgcgtgt ttgatgccat ctccgacccg gtgatgggcg tcattgccga
    30361 ccggacgcaa agtcgctggg ggcgctttcg tccgtggcag ttgtggattg ccattcccat
    30421 cggcattatc ggcatcctga cgttcaccgt gccagatgcc agcatgggag taaaaatcgc
    30481 ctgggcgttc ggcacttacc tgctcctttc agtcggttat accgccatca acgtgccgta
    30541 ctgcgcgctg atcaacacca tgaccacccg ccacaatgaa gtgatctcct gccagtcctg
    30601 gcgattcgtt ctctgcggcg tggcgggatt tctggtttcg gtaggcttac cgtggatggt
    30661 agatctcttc ggtcagggta atgccgcgcg cggctatcaa ctgggcgtcg gcgtgctgtg
    30721 cgccatcgct gtggtgatgt tcctgtgctg tttcttctgg gttcgtgaac gggtgccact
    30781 ctccacaatg gggaaattta ccctgcgcga acatcttgcc gggctgcgga acaacgacca
    30841 actgctgctg atgctggtca tgtctttcct gctgattaac gtctttaata ttcgcggcgg
    30901 tgggtatatg tacttcatta cctacgtctt acaaggcagt acgggctaca cgtcgctgtt
    30961 cttcactatg gtcaccttcg cctccattat cggctcggtg attgtcagcc cgttaacgcg
    31021 gcgtttcgat accgtcaaaa tttattacta caccaacctg ctcctcgctg cgctggcggt
    31081 gttgatgtgg ttcctgccct ccagcccggc ttatcaaacg ctgtggctgg cggtgatcct
    31141 cggtaatggc gtgattcttg gcttcacatt gccattgcac ttctcattga tggcctttgc
    31201 cgatgactac ggcgagtgga aaacccgcgt acgttcttcc ggcatgaact tcgccttcaa
    31261 tctgtttttc atcaagctgg cctgggcctc cagcgccggg atcatcagcc tgctgtttat
    31321 ttttgtcgcc taccagccag gcgtggaaaa ccagaccgcc agttcgcttg gcgggatcac
    31381 agcaatggaa acattgctgc ctgcgctgtt ccacctgctg ctggcgatgg cgatccgctt
    31441 ttgcaaactc aataacccta tgatgtcacg cattgctagc gacctgcgtc agcgtcatgt
    31501 acagccttaa ggagcacaag atgaacattt cggaagtcga tctgcataaa ctgacggtca
    31561 gcgatccgtt cctcggtcag taccaacaac tggtccgcga tgtggtgatt ccttatcagt
    31621 gggacgcctt gaacgatcgt atcccagaag cggaacccag ccatgcgatt gaaaactttc
    31681 gcattgccgc aggactgcaa gacggtgaat tttacgggat ggtgtttcag gacagcgacg
    31741 tcgccaaatg gctggaagcg gtagcctggt cgctgtgcca gaagccggac gccgaactgg
    31801 aaaaaaccgc cgacgaggtg attgaactga tcgcctccgc ccagtgtgaa gatggctatc
    31861 tcaatactta ctttacggta aaagcgcccg aagaacgctg gagcaatctg gcggagtgtc
    31921 atgaacttta ctgcgcgggt catctgattg aagccggagt cgccttcttc caggctacgg
    31981 gcaagcggcg cttgctggaa gtggtttgcc gtctgaccga tcatatcgac agcgtatttg
    32041 gtccagatga aagtaagtta cacggttatc ctggtcaccc ggaaattgaa ctggcactaa
    32101 tgcgcctgta tgaagtgacc gaagagccgc gctacctggc gctgacgaac tattttgtcg
    32161 aacagcgtgg tgcgcaaccg cactattacg accaggaata tgaaaagcgc gggcagacct
    32221 cgcactggca cacctacggc ccggcgtgga tggtgaaaga caaagcctac agccaggcac
    32281 atttgcccat cgcacagcag caaaccgcca ttggtcacgc ggtacgtttt gtctacctga
    32341 tgaccggcgt cgcgcatctc gcgcgtttaa gtcacgatga aagcaagcgt caggattgct
    32401 tgcggctgtg gaacaatatg gcccagcgtc agttatatat taccggcggc attggctcac
    32461 agagcagcgg tgaagcgttc agcagcgatt acgatctgcc gaatgacacg gtatacgccg
    32521 aaagctgtgc ttccatcggc ctgatgatgt tcgcccggcg aatgctggaa atggaaggcg
    32581 acagtcaata tgccgatgtg atggagcgcg cactgtacaa caccgtgctc ggcggcatgg
    32641 cgctggatgg caaacatttc ttctatgtga atccgctgga agtacatcca aaatcgctga
    32701 aattcaacca tatctacgat cacgttaagc cgatccgcca gcgttggttt ggttgcgctt
    32761 gttgtccgcc aaatatcgcc cgcgtgctaa cctcgattgg tcattatctc tacacgccgc
    32821 gtgaagatgc gttgtatatc aacatatacg caggaaacag catggaagtg ccggtagaaa
    32881 atggcacgtt gcgcctgcgg gttagcggaa actatccgtg gcaggaacag gtgacaattg
    32941 cggttgaatc gccccagccg gtgcgtcata cgctggcttt acgtctgccg gactggtgca
    33001 cacagccgca gattacattg aatggggaag aggtcgagca ggatattcgt aaagggtatt
    33061 tgcacattac ccgcgaatgg caggagggcg acacgctgaa tctgacgt
//
LOCUS       DAMZFT010000046        31971 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.046, whole genome shotgun sequence.
ACCESSION   DAMZFT010000046 DAMZFT010000000
VERSION     DAMZFT010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 31971)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31971)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31971
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.046"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(<1..478)
                     /gene="basR"
                     /locus_tag="PN977_002221"
     CDS             complement(<1..478)
                     /gene="basR"
                     /locus_tag="PN977_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BasR"
                     /protein_id="HDI6042250.1"
                     /translation="MKILIVEDDTLLLQGLILAAQTEGYACDGVTTARMAEQSLEAGH
                     YSLVVLDLGLPDEDGLHFLARIRQKKYTLPVLILTARDTLTDKIAGLDVGADDYLVKP
                     FALEELHARIRALLRRHNNQGESELIVGNLTLNMGRRQVWMGGEELILTPKEYALLS"
     gene            complement(475..2118)
                     /gene="eptA"
                     /locus_tag="PN977_002222"
     CDS             complement(475..2118)
                     /gene="eptA"
                     /locus_tag="PN977_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase EptA"
                     /protein_id="HDI6042251.1"
                     /translation="MLKRLLKRPSLNLLAWLLLAAFYISICLNIAFFKQVLQALPLDS
                     LHNVLVFLSMPVVAFSVINIVLTLSSFLWLNRPLACLFILVGAAAQYFIMTYGIVIDR
                     SMIANIIDTTPAESYALMTPQMLLTLGFSGVLAALIACWIKIKPATSRLRSVLFRGAN
                     ILVSVLLILLVAALFYKDYASLFRNNKELVKSLSPSNSIVASWSWYSHQRLANLPLVR
                     IGEDAHRNPLMQNETRKNLTILIVGETSRAENFSLNGYPRETNPRLAKDNVVYFPNTA
                     SCGTATAVSVPCMFSDMPREHYKEELAQHQEGVLDIIQRAGINVLWNDNDGGCKGVCD
                     RVPHQNVTALNLPGQCINGECYDEVLFHGLEDYINNLQGDGVIVLHTIGSHGPTYYNR
                     YPPQFRKFTPTCDTNEIQTCTQEQLVNTYDNTLVYVDYIVDKAINLLKEHQDKFTTSL
                     VYLSDHGESLGENGIYLHGLPYAIAPDSQKQVPMLLWLSEDYQKRYQVDQNCLQKQAQ
                     TQHYSQDNLFSTLLGLTGVETKYYQAADDILQTCRRVSE"
     gene            complement(2222..3559)
                     /gene="adiC"
                     /locus_tag="PN977_002223"
     CDS             complement(2222..3559)
                     /gene="adiC"
                     /locus_tag="PN977_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine/agmatine antiporter"
                     /protein_id="HDI6042252.1"
                     /translation="MSSDADAHKVGLIPVTLMVSGNIMGSGVFLLPANLASTGGIAIY
                     GWLVTIIGALGLSMVYAKMSFLDPSPGGSYAYARRCFGPFLGYQTNVLYWLACWIGNI
                     AMVVIGVGYLSYFFPILKDPLVLTITCVVVLWIFVLLNIVGPKMITRVQAVATVLALI
                     PIVGIAVFGWFWFRGETYMAAWNVSGLGTFGAIQSTLNVTLWSFIGVESASVAAGVVK
                     NPKRNVPIATIGGVLIAAVCYVLSTTAIMGMIPNAALRVSASPFGDAARMALGDTAGA
                     IVSFCAAAGCLGSLGGWTLLAGQTAKAAADDGLFPPIFARVNKAGTPVAGLIIVGILM
                     TIFQLSSISPNATKEFGLVSSVSVIFTLVPYLYTCAALLLLGHGHFGKARPAYLAVTT
                     IAFLYCIWAVVGSGAKEVMWSFVTLMVITAMYALNYNRLHKNPYPLDAPISKD"
     gene            complement(3696..4457)
                     /gene="adiY"
                     /locus_tag="PN977_002224"
     CDS             complement(3696..4457)
                     /gene="adiY"
                     /locus_tag="PN977_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator AdiY"
                     /protein_id="HDI6042253.1"
                     /translation="MRICSDQPCIVLLTEKDVWIRVNGKEPISLKANHMALLNCENNI
                     IDVSSLNNTLVAHISHDIIKDYLRFLNKDLSQIPVWQRSATPILTLPCLTPDVFRVAA
                     QHSMMPAETESEKERTRALLFTVLSRFLDSKKFLSLMMYMLRNCVSDSVYQIIESDIH
                     KDWNLSMVASCLCLSPSLLKKKLKSENTSYSQIITTCRMRYAVNELMMDGKNISQVSQ
                     SCGYNSTSYFISVFKDFYGMTPLHYVSQHRERTVA"
     gene            complement(4793..7060)
                     /gene="adiA"
                     /locus_tag="PN977_002225"
     CDS             complement(4793..7060)
                     /gene="adiA"
                     /locus_tag="PN977_002225"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418541.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase"
                     /protein_id="HDI6042254.1"
                     /translation="MKVLIVESEFLHQDTWVGNAVERLADALSQQNVTVIKSTSFDDG
                     FAILSSNEAIDCLMFSYQMEHPDEHQNVRQLIGKLHERQQNVPVFLLGDREKALAAMD
                     RDLLELVDEFAWILEDTADFIAGRAVAAMTRYRQQLLPPLFSALMKYSDIHEYSWAAP
                     GHQGGVGFTKTPAGRFYHDYYGENLFRTDMGIERTSLGSLLDHTGAFGESEKYAARVF
                     GADRSWSVVVGTSGSNRTIMQACMTDNDVVVVDRNCHKSIEQGLMLTGAKPVYMVPSR
                     NRYGIIGPIYPQEMQPETLQKKINESPLTKDKAGQKPSYCVVTNCTYDGVCYNAKEAQ
                     DLLEKTSDRLHFDEAWYGYARFNPIYADHYAMRGEPGDHNGPTVFATHSTHKLLNALS
                     QASYIHVREGRGAINFSRFNQAYMMHATTSPLYAICASNDVAVSMMDGNSGLSLTQEV
                     IDEAVDFRQAMARLYKEFTADGSWFFKPWNKEVVTDPQTGKTYDFADAPTKLLTTVQD
                     CWVMHPGESWHGFKDIPDNWSMLDPIKVSILAPGMGEDGELEETGVPAALVTAWLGRH
                     GIVPTRTTDFQIMFLFSMGVTRGKWGTLVNTLCSFKRHYDANTPLAQVMPELVEQYPD
                     TYANMGIHDLGDTMFAWLKENNPGARLNEAYSGLPVAEITPREAYNAIVDNNVELVSI
                     ENLPGRIAANSVIPYPPGIPMLLSGENFGDKNSPQVSYLRSLQSWDHHFPGFEHETEG
                     TEIIDGIYHVMCVKA"
     gene            complement(7259..8167)
                     /gene="melR"
                     /locus_tag="PN977_002226"
     CDS             complement(7259..8167)
                     /gene="melR"
                     /locus_tag="PN977_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator MelR"
                     /protein_id="HDI6042255.1"
                     /translation="MNTDTFMCSSDEKQTRSPLSLYSEYQRMEIEFRAPHIMPTSHWH
                     GQVEVNVPFDGDVEYLINNEKVNINQGHITLFWACTPHQLTDTGTCQSMAIFNLPMHL
                     FLSWPLDKDLINHVTHGMVIKSLATQQLSPFEVRRWQQELNSPNEQIRQLAIDEIGLM
                     LKRFSLSGWEPILVNKTSRTHKNSVSRHAQFYVSQMLGFIAENYDQALTINDVAEHVK
                     LNANYAMGIFQRVMQLTMKQYITAMRINHVRALLSDTDKSILDIALTAGFRSSSRFYS
                     TFGKYVGMSPQQYRKLSQQRRQVFPG"
     gene            8450..9805
                     /gene="melA"
                     /locus_tag="PN977_002227"
     CDS             8450..9805
                     /gene="melA"
                     /locus_tag="PN977_002227"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HDI6042256.1"
                     /translation="MMSAPKITFIGAGSTIFVKNILGDVFHREALKTAHIALMDIDPT
                     RLEESHIVVRKLMDSAGASGKITCHTQQKEALQDADFVVVAFQIGGYEPCTVTDFEVC
                     KRHGLEQTIADTLGPGGIMRALRTIPHLWQICEDMTEVCPDATMLNYVNPMAMNTWAM
                     YARYPHIKQVGLCHSVQGTAEELARDLNIDPATLRYRCAGINHMAFYLELERKTADGS
                     YVNLYPELLAAYDAGQAPKPNIHGNTRCQNIVRYEMFKKLGYFVTESSEHFAEYTPWF
                     IKPGREDLIERYKVPLDEYPKRCVEQLANWHKELEEYKNASRIDIKPSREYASTIMNA
                     IWTGEPSVIYGNVRNDGLIDNLPQGCCVEVACLVDANGIQPTKVGTLPSHLAALMQTN
                     INVQTLLTEAILTENRDRVYHAAMMDPHTAAVLGIDEIYALVDDLIAAHGDWLPGWLH
                     R"
     gene            9908..11329
                     /gene="melB"
                     /locus_tag="PN977_002228"
     CDS             9908..11329
                     /gene="melB"
                     /locus_tag="PN977_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418544.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="melibiose:sodium transporter MelB"
                     /protein_id="HDI6042257.1"
                     /translation="MSISMTTKLSYGFGAFGKDFAIGIVYMYLMYYYTDVVGLSVGLV
                     GTLFLVARIWDAINDPIMGWIVNATRSRWGKFKPWILIGTLANSVILFLLFSAHLFEG
                     TTQIVFVCVTYILWGMTYTIMDIPFWSLVPTITLDKREREQLVPYPRFFASLAGFVTA
                     GVTLPFVNYVGGGDRGFGFQMFTLVLIAFFIVSTIITLRNVHEVFSSDNQPSAEGSHL
                     TLKAIVALIYKNDQLSCLLGMALAYNVASNIITGFAIYYFSYVIGDADLFPYYLSYAG
                     AANLVTLVFFPRLVKSLSRRILWAGASILPVLSCGVLLLMALMSYHNVVLIVIAGILL
                     NVGTALFWVLQVIMVADTVDYGEYKLHVRCESIAYSVQTMVVKGGSAFAAFFIAVVLG
                     MIGYVPNVEQSTQALLGMQFIMIALPTLFFMVTLILYFRFYRLNGDTLRRIQIHLLDK
                     YRKVPPEPVHADIPVGAVSDVKA"
     gene            complement(11468..12097)
                     /locus_tag="PN977_002229"
     CDS             complement(11468..12097)
                     /locus_tag="PN977_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2238 domain-containing protein"
                     /protein_id="HDI6042258.1"
                     /translation="MTRTLKPLILNTGALALTLILIYTGISAHDKLTWLMEVTPVIIV
                     VPLLLATAKRYPLTPLLYTLIFFHAIILMVGGQYTYAKVPVGFEVQEWLGLSRNPYDK
                     LGHFFQGLVPALVAREILVRGMYVRGRKMVAFLVCCVALAISAMYELIEWWAALAMGQ
                     GADDFLGTQGDQWDTQSDMFCALLGALTTVTLLAGTHCRQLRRYGLITG"
     gene            complement(12220..13866)
                     /gene="fumB"
                     /locus_tag="PN977_002230"
     CDS             complement(12220..13866)
                     /gene="fumB"
                     /locus_tag="PN977_002230"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HDI6042259.1"
                     /translation="MSNKPFIYQAPFPMGKDNTEYYLLTSDYVSVADFDGETILKVEP
                     EALTLLAQQAFHDASFMLRPAHQKQVAAILHDPEASENDKYVALQFLRNSEIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGGDEEALSKGVYNTYIEDNLRYSQNAPLDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCVSKGGGSANKTYLYQETKALLTPGKLKNFLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAHYYDELPTEGNEHGQAFRDIQLEQEL
                     LEEAQKLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINREGIW
                     IEKLEHNPGQYIPEELRKAGEGEAVKVDLNRPMKEILAQLSQYPVSTRLSLTGTIIVG
                     RDIAHAKLKELIDAGKELPQYIKDHPIYYAGPAKTPAGYPSGSLGPTTAGRMDSYVDL
                     LQSHGGSMIMLAKGNRSQQVTDACHKHGGFYLGSIGGPAAVLAQQSIKHLECVAYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIVNKQCANCTK"
     gene            complement(13944..15284)
                     /gene="dcuB"
                     /locus_tag="PN977_002231"
     CDS             complement(13944..15284)
                     /gene="dcuB"
                     /locus_tag="PN977_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuB"
                     /protein_id="HDI6042260.1"
                     /translation="MLFTIQLIIILICLFYGARKGGIALGLLGGIGLVILVFVFHLQP
                     GKPPVDVMLVIIAVVAASATLQASGGLDVMLQIAEKLLRRNPKYVSIVAPFVTCTLTI
                     LCGTGHVVYTILPIIYDVAIKNNIRPERPMAASSIGAQMGIIASPVSVAVVSLVAMLG
                     NVTFDGRHLEFLDLLAITIPSTLIGILAIGIFSWFRGKDLDKDEEFQKFISVPENREY
                     VYGDTATLLDKKLPKSNWLAMWIFLGAIAVVALLGADSDLRPSFGGKPLSMVLVIQMF
                     MLLTGALIIILTKTNPASISKNEVFRSGMIAIVAVYGIAWMAETMFGAHMSEIQGVLG
                     EMVKEYPWAYAIVLLLVSKFVNSQAAALAAIVPVALAIGVDPAYIVASAPACYGYYIL
                     PTYPSDLAAIQFDRSGTTHIGRFVINHSFILPGLIGVSVSCVFGWIFAAMYGFL"
     gene            complement(15855..16574)
                     /gene="dcuR"
                     /locus_tag="PN977_002232"
     CDS             complement(15855..16574)
                     /gene="dcuR"
                     /locus_tag="PN977_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator DcuR"
                     /protein_id="HDI6042261.1"
                     /translation="MINVLIIDDDAMVAELNRRYVAQIPGFQCCGTASTLEKAKEIIF
                     NSDTPIDLILLDIYMQKENGLDLLPVLHNARCKSDVIVISSAADAATIKDSLHYGVVD
                     YLIKPFQASRFEEALTGWRQKKMALEKHQYYDQAELDQLIHGSSSNEQDPRRLPKGLT
                     PQTLRTLCQWIDAHQDYEFSTDELANEVNISRVSCRKYLIWLVNCHILFTSIHYGVTG
                     RPVYRYRIQAEHYSLLKQYCQ"
     gene            complement(16571..18202)
                     /gene="dcuS"
                     /locus_tag="PN977_002233"
     CDS             complement(16571..18202)
                     /gene="dcuS"
                     /locus_tag="PN977_002233"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     DcuS"
                     /protein_id="HDI6042262.1"
                     /translation="MRHSLPYRMLRKRPMKLSTTVILMVSAVLFSVLLVVHLIYFSQI
                     SDMTRDGLANKALAVARTLADSPEIRQGLQKKPQESGIQAIAEAVRKRNDLLFIVVTD
                     MQSLRYSHPEAQRIGQPFKGDDILKALNGEENVAINRGFLAQALRVFTPIYDENHKQI
                     GVVAIGLELSRVTQQINDSRWSIIWSVLFGMLVGLIGTCILVKVLKKILFGLEPYEIS
                     TLFEQRQAMLQSIKEGVVAVDDRGEVTLINDAAQELLNYRKSQDDEKLSTLSHAWSQV
                     VDVSEVLRDGTPRRDEEITIKDRLLLINTVPVRSNGVIIGAISTFRDKTEVRKLMQRL
                     DGLVNYADALRERSHEFMNKLHVILGLLHLKSYKQLEDYILKTANNYQEEIGSLLGKI
                     KSPVIAGFLISKINRATDLGHTLILNSESQLPDSGSEDQVATLITTLGNLIENALEAL
                     GPEPGGEISVTLHYRHGWPHCEVNDDGPGIAPDKIDHIFDKGVSTKGSERGVGLALVK
                     QQVENLGGSIAVESEPGIFTQFFVQIPWDGERSNR"
     gene            18383..18613
                     /locus_tag="PN977_002234"
     CDS             18383..18613
                     /locus_tag="PN977_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(4Fe-4S)-binding protein"
                     /protein_id="HDI6042263.1"
                     /translation="MDQALLDGGYRCYTGENIDVYFNTAICQHSGNCVRGNGKLFNLK
                     RKPWIMPDEVDVATVVKVIDTCPSGALKYRHK"
     gene            18625..18897
                     /locus_tag="PN977_002235"
     CDS             18625..18897
                     /locus_tag="PN977_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HDI6042264.1"
                     /translation="MEIREGHNKFYINDEQGKQIAEIVFVPTGENLAIIEHTDVDESL
                     KGQGIGKQLVAKVVEKMRREKRKIIPLCPFAKHEFDKTREYDDIRS"
     gene            19126..19422
                     /gene="ghoS"
                     /locus_tag="PN977_002236"
     CDS             19126..19422
                     /gene="ghoS"
                     /locus_tag="PN977_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system endoribonuclease
                     antitoxin GhoS"
                     /protein_id="HDI6042265.1"
                     /translation="MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEY
                     GIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDIDFDLNIMTVDDYFRQFYK"
     gene            19450..19623
                     /gene="ghoT"
                     /locus_tag="PN977_002237"
     CDS             19450..19623
                     /gene="ghoT"
                     /locus_tag="PN977_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system toxin GhoT"
                     /protein_id="HDI6042266.1"
                     /translation="MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITW
                     PMSLPVALLFSLF"
     gene            complement(19742..21259)
                     /gene="lysS"
                     /locus_tag="PN977_002238"
     CDS             complement(19742..21259)
                     /gene="lysS"
                     /locus_tag="PN977_002238"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HDI6042267.1"
                     /translation="MSERETRGANEAIDFNDELRNRREKLAALRQQGVAFPNDFRRDH
                     TSDQLHDEFDAKDNQELESLNIEVSVAGRMMTRRIMGKASFVTLQDVGGRIQLYVARD
                     SLPEGVYNDQFKKWDLGDIIGARGTLFKTQTGELSIHCTELRLLTKALRPLPDKFHGL
                     QDQEVRYRQRYLDLIANDKSRQTFVVRSKILAAIRQFMVARGFMEVETPMMQVIPGGA
                     SARPFITHHNALDLDMYLRIAPELYLKRLVVGGFERVFEINRNFRNEGISVRHNPEFT
                     MMELYMAYADYHDLIELTESLFRTLAQEVLGTTKVTYGEHVFDFGKPFEKLTMREAIK
                     KYRPETDMADLDNFDAAKALAESIGITVEKSWGLGRIVTEIFDEVAEAHLIQPTFITE
                     YPAEVSPLARRNDVNPEITDRFEFFIGGREIGNGFSELNDAEDQAERFQEQVNAKAAG
                     DDEAMFYDEDYVTALEYGLPPTAGLGIGIDRMIMLFTNSHTIRDVILFPAMRPQK"
     gene            complement(21496..22953)
                     /gene="dtpC"
                     /locus_tag="PN977_002239"
     CDS             complement(21496..22953)
                     /gene="dtpC"
                     /locus_tag="PN977_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpC"
                     /protein_id="HDI6042268.1"
                     /translation="MKTPSQPRAIYYIVAIQIWEYFSFYGMRALLILYLTHQLGFDDN
                     HAISLFSAYASLVYVTPILGGWLADRLLGNRTAVIAGALLMTLGHVVLGIDTNSTFSL
                     YLALAIIICGYGLFKSNISCLLGELYDENDHRRDGGFSLLYAAGNIGSIAAPIACGLA
                     AQWYGWHVGFALAGGGMFIGLLIFLSGHRHFQSTRSMDKKALTSVKFALPVWTWLVVM
                     LCLAPVFFTLLLENDWSGYLLAIVCLIAAQIIARMMIKFPEHRRALWQIVLLMFVGTL
                     FWVLAQQGGSTISLFIDRFVNRQAFNIEVPTALFQSVNAIAVMLAGVVLAWLASPESR
                     GNSALRVWLKFAFGLLLMACGFMLLAFDARHAAAGGQASMGVMISGLALMGFAELFID
                     PVAIAQITRLKMSGVLTGIYMLATGAVANWLAGVVAQQTTESQISGMAIAAYQRFFSQ
                     MGEWTLACVAIIVVLAFATRFLFSTPTNMIQESND"
     gene            complement(23012..25159)
                     /gene="cadA"
                     /locus_tag="PN977_002240"
     CDS             complement(23012..25159)
                     /gene="cadA"
                     /locus_tag="PN977_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylase CadA"
                     /protein_id="HDI6042269.1"
                     /translation="MNVIAILNHMGVYFKEEPIRELHRALERLNFQIVYPNDRDDLLK
                     LIENNARLCGVIFDWDKYNLELCEEISKMNENLPLYAFANTYSTLDVSLNDLRLQISF
                     FEYALGAAEDIANKIKQTTDEYINTILPPLTKALFKYVREGKYTFCTPGHMGGTAFQK
                     SPVGSLFYDFFGPNTMKSDISISVSELGSLLDHSGPHKEAEQYIARVFNADRSYMVTN
                     GTSTANKIVGMYSAPAGSTILIDRNCHKSLTHLMMMSDVTPIYFRPTRNAYGILGGIP
                     QSEFQHATIAKRVKETPNATWPVHAVITNSTYDGLLYNTDFIKKTLDVKSIHFDSAWV
                     PYTNFSPIYEGKCGMSGGRVEGKVIYETQSTHKLLAAFSQASMIHVKGDVNEETFNEA
                     YMMHTTTSPHYGIVASTETAAAMMKGNAGKRLINGSIERAIKFRKEIKRLRTESDGWF
                     FDVWQPDHIDTTECWPLRSDSTWHGFKNIDNEHMYLDPIKVTLLTPGMEKDGTMSDFG
                     IPASIVAKYLDEHGIVVEKTGPYNLLFLFSIGIDKTKALSLLRALTDFKRAFDLNLRV
                     KNMLPSLYREDPEFYENMRIQELAQNIHKLIVHHNLPDLMYRAFEVLPTMVMTPYAAF
                     QKELHGMTEEVYLDEMVGRINANMILPYPPGVPLVMPGEMITEESRPVLEFLQMLCEI
                     GAHYPGFETDIHGAYRQADGRYTVKVLKEESKK"
     gene            complement(25239..26573)
                     /gene="cadB"
                     /locus_tag="PN977_002241"
     CDS             complement(25239..26573)
                     /gene="cadB"
                     /locus_tag="PN977_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadaverine/lysine antiporter"
                     /protein_id="HDI6042270.1"
                     /translation="MSSAKKIGLFACTGVVAGNMMGSGIALLPANLASIGGIAIWGWI
                     ISIIGAMSLAYVYARLATKNPQQGGPIAYAGEISPAFGFQTGVLYYHANWIGNLAIGI
                     TAVSYLSTFFPVLNDPVPAGIACIAIVWVFTFVNMLGGTWVSRLTTIGLVLVLIPVVM
                     TAIVGWHWFDAATYAANWNTADTTDGHAIIKSILLCLWAFVGVESAAVSTGMVKNPKR
                     TVPLATMLGTGLAGIVYIAATQVLSGMYPSSVMAASGAPFAISASTILGNWAAPLVSA
                     FTAFACLTSLGSWMMLVGQAGVRAANDGNFPKVYGEVDSNGIPKKGLLLAAVKMTALM
                     ILITLMNSAGGKASDLFGELTGIAVLLTMLPYFYSCVDLIRFEGVNIRNFVSLICSVL
                     GCVFCFIALMGASSFELAGTFIVSLIILMFYARKMHERQSHSMDNHTASNAH"
     gene            complement(26939..28477)
                     /gene="cadC"
                     /locus_tag="PN977_002242"
     CDS             complement(26939..28477)
                     /gene="cadC"
                     /locus_tag="PN977_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylation/transport transcriptional
                     activator CadC"
                     /protein_id="HDI6042271.1"
                     /translation="MQQPVVRVGEWLVTPSINQISRNGRQLTLEPRLIDLLVFFAQHS
                     GEVLSRDELIDNVWKRSIVTNHVVTQSISELRKSLKDNDEDSPVYIATVPKRGYKLMV
                     PVIWYSEEEGEEIMLSSPPPIPEAVPATDSPSHSLNIQNTTAPPEQSPVKSKRFTTFW
                     VWFFFLLSLGICVALVAFSSLETRLPMSKSRILLNPRDIDINMVNKSCNSWSSPYQLS
                     YAIGVGDLVATSLNTFSTFMVHDKINYNIDEPSSSGKTLSIAFVNQRQYRAQQCFMSV
                     KLVDNADGSTMLDKRYVITNGNQLAIQNDLLQSLSKALNQPWPQRMQETLQQILPHRG
                     ALLTNFYQAHNYLLHGDDKSLNRASELLGEIVQSSPEFTYARAEKALVDIVRHSQHPL
                     DEKQLAALNTEIDNIVTLPELNNLSIIYQIKAVSALVKGKTDESYQAINTGIDLEMSW
                     LNYVLLGKVYEMKGMNREAADAYLTAFNLRPGANTLYWIENGIFQTSVPYVVPYLDKF
                     LASE"
     gene            complement(29360..29686)
                     /locus_tag="PN977_002243"
     CDS             complement(29360..29686)
                     /locus_tag="PN977_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000779484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HDI6042272.1"
                     /translation="MKLTVSPLAEHDLEAIGDWIAQDNPVRAISFTEELYQQCLLIAE
                     SPVLYRERPELGAGIRGCSYGRYLLLFRVLDTEVRIERIVHGSRDINRVLAEPDDKPS
                     DHFWQP"
     gene            complement(29683..29946)
                     /locus_tag="PN977_002244"
     CDS             complement(29683..29946)
                     /locus_tag="PN977_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001143297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system ParD family
                     antitoxin"
                     /protein_id="HDI6042273.1"
                     /translation="MPTSVSLSPYFETFIREQIESGRYNNTSEVIRAGLRALEEREQQ
                     IKLESLQSAVTAGINSGESKSAEEVFGRLTHKYKKMAEGEQPI"
     gene            complement(30018..30884)
                     /locus_tag="PN977_002245"
     CDS             complement(30018..30884)
                     /locus_tag="PN977_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
                     /protein_id="HDI6042274.1"
                     /translation="MSDITASRPEVVNGHTDVICSTSIRHILAVRKSTLLQIDTLIRQ
                     LTEISVLTESIGGKTARDWAMKQDFRCGCWLMDKPETAMKAITRNLDREIWRDLMQRS
                     GMLSLMDAQARDTWYRSLEYDNFPEISEANILSTFEQLHQNKDEVFERGVINVFRGLS
                     WNYENNSPCKFGSKIIVNNLVRWDRWGFHLITGQQADRLADLERMLYLFSGKPIPDNR
                     ENITIHLDDHIQSVQGKEDYEDEMFSIRYFKKGSAHITFRKPELVDRLNDIIARHYSE
                     ALASTVNKSRKA"
     gene            complement(30969..31166)
                     /locus_tag="PN977_002246"
     CDS             complement(30969..31166)
                     /locus_tag="PN977_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6042275.1"
                     /translation="MKSLTTETALDILIVWLQDNIDCESGIIFDNDEDKTDSAALLPC
                     IERAREDVRTLRHLQLLHQNR"
     gene            complement(31178..31666)
                     /locus_tag="PN977_002247"
     CDS             complement(31178..31666)
                     /locus_tag="PN977_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001392970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6042276.1"
                     /translation="MKLALTLEADSINVQALNMGRIVVDVDGVTLAELINVVCDNGYS
                     LRVVDESDRASAERTPPSAALTGIRCSTAHITETDNAWLYSLSHQTNDFGESEWIHFT
                     GSGYLLRTDAWSYPVLRLKRLGLSKTFRRLVVTLIRRYGVSLIHLDASAECLPDLPTF
                     DW"
     gene            complement(31663..>31971)
                     /locus_tag="PN977_002248"
     CDS             complement(31663..>31971)
                     /locus_tag="PN977_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001757701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin"
                     /protein_id="HDI6042277.1"
                     /translation="TLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCNAVNFLV
                     EKYALVRTDQPGFSACTRSQLINSIDILRARRATGLMARDNYRTVNNITLGKHPGAKQ
                     "
BASE COUNT         8211 a         7595 c         7852 g         8313 t
ORIGIN      
        1 gtgacagcag agcatattct ttgggcgtca gaatcaactc ttcaccgccc atccatacct
       61 gacggcgacc catattcagc gtcagattgc caacaatcag ctcactttcg ccctgattat
      121 tatggcgtcg tagcagggcg cggatacggg cgtgtaactc ttccagcgca aaaggcttca
      181 ccagatagtc atcggcaccg acatccagcc cggcgatttt gtcggtcagc gtatcgcgag
      241 cggtgaggat cagtaccggc agggtgtatt ttttctgccg gatacgggcg agaaaatgca
      301 gtccatcttc gtcgggtaac cctaaatcca gtaccaccag gctgtaatga ccggcctcaa
      361 ggctttgttc cgccatccgc gcggttgtca cgccatcgca cgcgtagcct tcggtttgcg
      421 ccgccagaat caatccctgc aataacagcg tatcgtcttc aacaatcaga attttcattc
      481 actcactctc ctgcaagttt gcagaatatc atccgcagcc tggtaatact tcgtctcaac
      541 gccagtcagg cccaataagg ttgagaataa attgtcttgt gaatagtgtt gcgtttgcgc
      601 ctgtttttgc aggcagttct ggtcaacctg ataccgtttt tgataatcct ccgacagcca
      661 cagcagcatc ggcacctgtt tttggctatc cggggcgatg gcataaggca gaccgtgcag
      721 atagatgcca ttttcaccta acgattcacc gtggtcagaa agataaacca ggctggtggt
      781 aaatttatcc tgatgttctt tcagcagatt aatcgcttta tcaacaatat agtcgacgta
      841 aaccagcgtg ttgtcgtaag tgttcaccag ttgctcttgg gtacaggtct ggatctcgtt
      901 agtgtcgcag gttggggtaa atttcctgaa ctgcggcgga tagcggttgt aataggtcgg
      961 gccgtggctg ccgatggtgt gtaagacaat cacgccatca ccctgcaggt tattgatgta
     1021 atcttccagc ccgtggaaca gtacttcatc atagcactcg ccgttgatgc actgaccagg
     1081 caggttcagc gcggtgacgt tctggtgagg tacgcgatcg caaacgcctt tacagccgcc
     1141 atcgttgtca ttccacagca cgttgatgcc cgctcgctga atgatatcca gcacgccttc
     1201 ctggtgctgt gccagctctt ctttgtagtg ctcacgcggc atatccgaga acatgcacgg
     1261 tactgaaact gccgttgccg tgccgcaaga tgcggtatta ggaaaataga ccacgttatc
     1321 ttttgccagc cgcgggttag tttcacgcgg gtagccgttg agggagaagt tctccgcccg
     1381 cgaggtttcg ccgacaatca ggatggtcaa atttttacgt gtctcgttct gcattaacgg
     1441 gttgcggtgc gcgtcttcac caattcgcac cagcggcaga tttgccagtc gctgatggga
     1501 gtaccatgac cagctggcaa caatgctgtt agaggggctt aaggatttca ccagctcttt
     1561 gttattgcga aacaacgagg cgtagtcttt ataaaacagt gcggcgacca gcaaaatcag
     1621 cagtacagaa accagaatat tggctccacg gaaaagaaca ctgcgcaggc gcgaggtggc
     1681 tggtttgatt ttaatccagc aggcaatcag cgcagcaagc acgccgctga atcccagcgt
     1741 taataacatt tgcggtgtca tcaacgcata actttctgcc ggagtggtat caataatatt
     1801 ggcaatcatc gagcggtcga tgacgatgcc gtaagtcatt atgaaatatt gtgcagctgc
     1861 gccgaccaga ataaacaggc aggccagcgg tcgattaagc cacaagaaag agcttagtgt
     1921 caggacaata ttaatcacgc tgaaggcgac gaccggcatc gacaagaaaa ccagtacgtt
     1981 atgcagcgag tccagcggca gcgcctgcaa cacctgttta aaaaaggcaa tattcaggca
     2041 gatagagata taaaaagcgg ctaacaatag ccaggcgagt aaattcaaag agggtctttt
     2101 tagtaggcgc ttcaacatca cggtgtttcc atcgaacaaa gtgcgcatat gctcgcaaag
     2161 caaaattaag ccaaccttaa gttcttaagg ttggctttta tgtgtgcttg attaagggga
     2221 attaatcttt gcttattggt gcatctaagg gatacgggtt tttatgcagc cggttgtaat
     2281 tcagcgcata catggcggtg atgaccatta gggtgacaaa tgaccacata acctctttcg
     2341 ccccggaccc caccacggcc cagatgcagt agaggaaggc aatggtagtc actgccagat
     2401 atgccgggcg tgctttacca aagtgaccgt ggccgagcag cagtaacgcc gcacaggtgt
     2461 aaagatatgg caccagtgta aagatgaccg acacggaaga caccagaccg aactctttag
     2521 tcgcgtttgg cgaaatgcta ctgagctgga agatggtcat caaaataccg acgataatca
     2581 gccccgccac tggcgtgccc gctttattta cacgggcaaa aatcggtggg aacagtccgt
     2641 catcggcagc ggctttcgcc gtttgacccg ccagcaacgt ccagccgccc agtgaaccta
     2701 agcaacccgc agctgcgcag aaggaaacaa tggccccggc ggtgtcaccc aacgccatcc
     2761 gtgcggcatc accgaatggc gaggcagaaa cgcgcagtgc ggcattaggg atcatcccca
     2821 taatcgcggt ggtggagagc acatagcaaa cggcggcaat caatacccca ccaatggtgg
     2881 cgatagggac gttgcgtttc gggtttttca ccacacctgc ggcaacggag gcactttcca
     2941 caccgatgaa cgaccacagc gtaacgttaa gggtactttg aatcgcgccg aaggtgccca
     3001 ggccgctgac gttccatgcc gccatatagg tttcaccacg gaaccagaac cagccaaata
     3061 cggcaatccc gacgatgggg atcagcgcca gcacggtggc aacggcctgc acgcgggtga
     3121 tcattttcgg accgacaatg ttcagcagga cgaagatcca cagcaccacg acgcaggtga
     3181 tggttaatac cagtggatct ttcagaatcg ggaagaagta acttaaatat cctacgccaa
     3241 tgaccaccat ggcgatattg ccgatccagc aggccagcca gtagaggacg ttggtttgat
     3301 aaccgagaaa cgggccaaag cagcggcggg cgtaagcgta agaaccacca ggacttgggt
     3361 cgaggaacga cattttggcg tataccatcg agagccccag cgcaccgata atcgtcacca
     3421 accatccata aatggcaatc ccgccagtgg aggccaggtt tgcaggtaac agaaaaacac
     3481 ctgaccccat aatattcccc gacaccatca gggtgacggg gattaagccc actttgtgag
     3541 catcagcatc cgaagacata attaaactcc tgcgaaggcg agcttcgtga caataaatta
     3601 cgtcatatca tacgcctgca ttcgatagtt gaattattat cggccgggtt atcgctgatg
     3661 ccgttattaa tatagctctt cgttaaggtt aaaaatcagg cgacagtgcg ttctctgtgc
     3721 tgactaacat aatgcagcgg cgtcataccg tagaagtctt taaagacaga aataaagtac
     3781 gacgtactgt tgtagccgca ggactgtgat acctgagaga tatttttacc gtccatcatt
     3841 aattcattta cggcataacg catgcggcag gtggtgatta tttggctata actggtgttt
     3901 tcgcttttca gctttttctt taacagactt gggctaagac ataaacaact ggctaccata
     3961 ctaagattcc agtctttgtg gatatcgctt tcaataattt gataaacgct gtcacttaca
     4021 caattacgta acatatacat cattaatgaa aggaattttt tactgtcgag aaaacgggat
     4081 agcacagtga ataataatgc acgtgttcgt tccttttctg actcagtttc agcgggcatc
     4141 atgctatgtt gcgcggcaac gcgaaagacg tctggcgtca ggcatggcag ggtgaggatg
     4201 ggcgtagcgc tacgttgcca tactggtatt tgcgagagat ctttattcag aaaccggagg
     4261 taatctttga tgatgtcgtg actaatatga gcaaccaaag tgttattaag agaggagacg
     4321 tcgataatat tattttcaca atttaataac gccatgtggt tagctttaag gctaataggt
     4381 tctttcccat tcacccttat ccagacatct ttttcagtca ataaaacaat acaaggttgg
     4441 tcgctgcaaa tcctcataca tgtactcctg agtgcgaata aaagaccagt tctcgctaaa
     4501 gcgaagcgat accgggaaat ggttatataa ttgtatgatg aatataaaca tgcataaaaa
     4561 taacgagttt tactaatatt acaaattttt atagtaattt ctcatgtaca tattttttta
     4621 caggctaaaa aaaggataaa taacaggcaa aaaaaagcgc ggggtgggac cgcgcaaaaa
     4681 gtatgacatt ttttgtttgc agcaattttt atgaaaacgt tgccatactt aaaaggcctc
     4741 aagctaggcg gcgtaatgtt atttaacaat tacgccttca gcggaatagt ggttacgctt
     4801 tcacacacat aacgtggtaa ataccgtcaa taatttctgt cccttctgtt tcgtgttcaa
     4861 atccagggaa atgatggtcc caggattgca gcgagcgtaa ataacttact tgcggactgt
     4921 ttttatcgcc gaagttttca ccagacagca gcatcgggat tcctggcgga tacgggataa
     4981 ctgagtttgc cgcgatacgt cctggcagat tttcaatgga taccagttcg acattgttgt
     5041 cgacaatcgc gttgtacgct tcacgcgggg tgatttccgc caccggtagg ccggaatagg
     5101 cttcattcaa ccgtgcgcca gggttgtttt ctttcagcca ggcaaacatg gtgtcaccca
     5161 gatcgtgaat ccccatgttc gcgtaagtgt caggatattg ttcaacaagt tccggcatca
     5221 cctgcgccag cggtgtgttg gcatcatagt ggcgtttgaa ggagcaaagg gtgttaacca
     5281 gagttcccca tttcccacgg gtcacgccca tagagaacag gaacataatc tggaagtcag
     5341 tggtacgggt aggcacaatg ccgtggcgac caagccaggc tgtgaccagc gccgccggaa
     5401 caccggtttc ttccagttca ccatcttcac ccattcccgg agcaaggatg ctgactttaa
     5461 tcgggtcgag catactccag ttatccggaa tatctttgaa gccgtgccag ctttcgcccg
     5521 gatgcatgac ccagcagtcc tgaacggtgg tcagcagttt ggttggtgcg tcagcaaagt
     5581 cataggtttt gccggtttgt gggtcggtga cgacttcttt gttccacggt ttgaagaacc
     5641 agctaccgtc agcggtgaac tctttatata gccgcgccat cgcctgacgg aaatcaaccg
     5701 cttcgtcgat cacttcctgg gtcagtgaca ggccgctgtt gccgtccatc atcgacaccg
     5761 ccacgtcgtt ggatgcgcag atggcataca gcggggaggt ggtggcatgc atcatgtagg
     5821 cctggttgaa gcgggagaag ttaatcgccc cacgaccttc acgtacatga atataagagg
     5881 cctgtgacag cgcattcagc agtttgtggg tggagtgggt ggcgaaaacg gtaggaccgt
     5941 tgtgatcgcc aggttcgccg cgcatggcat agtgatcggc atagatcggg ttgaaacgtg
     6001 catagccgta ccaggcttcg tcaaagtgca gacgatcgga ggttttttcc agcagatcct
     6061 gtgcttcttt agcgttataa cacacgccgt cataggtgca gttggtcacc acgcagtaag
     6121 acggtttttg cccggctttg tctttagtca gcgggctttc attgattttc ttctgcaagg
     6181 tttcaggttg catttcctgc ggatagattg gcccgataat gccgtagcgg ttgcggcttg
     6241 gcaccatgta gaccggtttc gcgcctgtca gcatcaagcc ttgttcgatg gatttatggc
     6301 agttacggtc aacgaccacg acatcgttat cggtcatgca agcctgcatg atggtgcggt
     6361 tagagccgga agtaccgacg actaccgacc aggagcgatc ggcaccaaat acgcgtgcgg
     6421 catatttttc gctttcgcca aatgcgccag tatggtcaag caaagaaccg agggaagtgc
     6481 gttcgatgcc catgtcggtg cggaacaggt tttcaccata gtagtcatgg tagaaacgtc
     6541 cggcgggtgt tttagtaaaa ccaacgccgc cctggtggcc tggcgctgcc caggaatatt
     6601 catggatgtc gctatatttc atcagcgcgc tgaacagtgg cggcaacagc tgctggcggt
     6661 agcgggtcat cgcggcaacg gcgcgtccgg cgataaagtc ggcggtatct tccagtatcc
     6721 aggcgaattc atcgacaagc tccagcagat cccggtccat tgcggcgagg gctttttccc
     6781 gatcacccaa caggaagacc ggcacgtttt gttggcgctc atgaagctta ccgatcaatt
     6841 gtctgacgtt ttgatgttcg tccggatgtt ccatttggta gctgaacatc aggcagtcaa
     6901 tggcttcgtt tgaagagaga atggcaaaac catcatcaaa ggaggtggat ttaatcacgg
     6961 taacattttg ctggcttaaa gcatctgcca gacgctcaac ggcgttaccg acccaggtgt
     7021 cttgatggag aaactcgctt tcaacaatta atactttcat cattgcttac ccggttatga
     7081 aggaatcttc gttgcgggca agtatcttcc ggccttttcg ggctgtaaag cgcgtgaaaa
     7141 acaaacattt ttgaaatatt tatttttata aacaatacgt tatgtgatta ttttaaatta
     7201 ttttcactgc ttattatcga cggctaaact atttttttgg ctgatactga tatcgtcttt
     7261 agccgggaaa cacctggcgg cgttgttggc taagtttgcg gtattgttgc ggcgacatgc
     7321 cgacatattt gccgaacgtg ctgtaaaaac ggctactcga acgaaagcct gccgtcaggg
     7381 caatatcgag aatactttta tcggtatcgc tcagtaacgc gcgaacgtgg ttgatgcgca
     7441 tcgcggtaat gtactgtttc attgtcaatt gcatgacccg ctggaatatc cccattgcat
     7501 agttggcgtt aagtttgacg tgctcagcca catcgttgat ggtcagcgcc tgatcatagt
     7561 tttcggcaat aaagcccagc atctggctga cataaaattg cgcatggcgc gagacgctgt
     7621 ttttgtgtgt gcgcgaggtt ttattgacca gaatcggttc ccagccagag aggctaaatc
     7681 gcttgagcat caggccaatt tcatcaatgg cgagctggcg aatttgctcg ttcggactgt
     7741 tcaattcctg ctgccagcgg cgcacttcaa acgggctaag ttgctgtgtc gccagtgatt
     7801 tgatcaccat gccgtgagta acgtggttaa tcaggtcttt atccagcggc caggagagaa
     7861 acagatgcat cggcagatta aaaatcgcca tgctctgaca ggttccggta tctgttagtt
     7921 ggtgcggtgt gcaggcccag aacagcgtga tatgaccctg attgatattc actttttcat
     7981 tgttgatcag gtattccaca tcgccatcga aaggcacatt cacttcgacc tgaccatgcc
     8041 agtggctggt gggcatgata tgtggtgcgc gaaactcaat ttccatccgc tggtattctg
     8101 aatacagcga cagtgggcta cgggtctgct tttcgtcgct gctgcacata aacgtatctg
     8161 tattcatgga tgactctctt tcctggaata tcagaattat ggcaggagtg agggaggttg
     8221 actgcgagtg ggagcacggt tttcactctc ttcccagagg ggcgagggga ccgtccgagc
     8281 attctgaggc cgaaaactct gctttttagc taatttattc ccataaactc agatttactg
     8341 atacttcacg caggatctga gtttatggga atgctcaacc tggaagccgg aggttttctg
     8401 cagattcgcc tgccatgatg aagtcattca agcaagccag gagatctgca tgatgtctgc
     8461 acctaaaatt acatttatcg gcgctggttc gacgattttc gttaaaaata ttcttggtga
     8521 tgtgttccat cgtgaggcgc tgaaaacggc gcatattgcc ctgatggata tcgatcccac
     8581 ccgcctggaa gagtcgcaca ttgtggtgcg taagctgatg gattcagcag gggccagcgg
     8641 caaaatcacc tgccacaccc aacagaaaga agccttacag gatgccgatt ttgtggtggt
     8701 ggcatttcag attggcggtt atgaaccttg cacggtgact gatttcgagg tctgtaagcg
     8761 gcatggtctg gaacaaacca ttgccgatac gttggggccg ggcggtatta tgcgcgcgct
     8821 acgtaccatt ccgcatctgt ggcaaatttg cgaggacatg acggaagtct gccccgatgc
     8881 caccatgctc aactacgtta acccaatggc gatgaatacc tgggcgatgt atgcccgcta
     8941 tccgcatatc aaacaggtag ggctgtgcca ttcggtgcag ggaacggcgg aagagctggc
     9001 gcgtgacctc aatatcgacc cggctacgct gcgttaccgt tgcgcaggta tcaaccatat
     9061 ggcgttttac ctggaactgg agcgcaaaac cgccgacggc agttacgtga atctctaccc
     9121 ggaactgctg gcagcgtatg acgcagggca ggcaccgaag ccgaatattc atggcaatac
     9181 tcgctgccag aatattgtgc gttatgaaat gttcaaaaag ctgggctact tcgtcacgga
     9241 atcgtcagaa cattttgctg aatacacacc gtggtttatt aagcctggtc gtgaggattt
     9301 gattgagcgt tataaagtac cgctggatga gtacccgaaa cgctgcgtcg aacaattggc
     9361 aaactggcat aaagagctgg aggagtataa gaacgcctcc cggattgata ttaaaccgtc
     9421 acgggaatat gccagcacaa tcatgaacgc tatctggact ggcgagccga gtgtgattta
     9481 cggcaacgtc cgtaacgatg gattgattga taacctgcca caaggatgtt gcgtggaagt
     9541 agcctgtctg gttgatgcta atggcattca gccgaccaaa gtcggtacgc taccttcgca
     9601 tctggccgcc ctgatgcaaa ccaacatcaa cgtacagacg ctgctgaccg aagctattct
     9661 tacggaaaat cgcgaccgtg tttaccacgc cgcgatgatg gacccgcata ctgccgccgt
     9721 gctgggcatt gacgaaatat atgctcttgt tgacgacctg attgccgccc acggcgactg
     9781 gctgccaggc tggttgcacc gttaaaacgc gactaaacgc tactgcgccg ggggatttat
     9841 tccggcgcac acctctgacg ataccaataa cagaaggcgg gcgttggtaa cagcgacccg
     9901 ataccctatg agcatttcaa tgactacaaa actcagttat ggatttggag cgttcgggaa
     9961 ggattttgcg atcggcattg tgtatatgta tctcatgtat tactacaccg atgtcgtcgg
    10021 actgtctgtg ggtttggtcg gtactttgtt tctggtggcg aggatctggg atgctattaa
    10081 cgatccgatt atgggatgga ttgtaaatgc tacgcgatcg cgatggggta agttcaaacc
    10141 ctggatcctg atcggtacgt tggcaaactc tgtaatctta tttctcctct ttagtgcgca
    10201 tctgtttgaa ggtactactc agattgtctt tgtttgcgtg acctacatcc tctggggcat
    10261 gacttacacc attatggata ttcccttctg gtcgctggtt ccaaccataa cgctcgataa
    10321 acgtgagcgc gaacaactgg ttccttatcc gcgttttttt gccagtctgg cgggctttgt
    10381 tacggcaggt gtgacgctac catttgttaa ttatgtcggc ggtggcgatc ggggatttgg
    10441 ctttcagatg ttcactctgg tactgatcgc cttttttatt gtttcaacca tcatcactct
    10501 gcgcaatgtg catgaagtct tttcgtcaga caatcagccg tctgctgaag gaagccatct
    10561 gacacttaaa gccatcgttg cgcttattta taaaaacgat cagctttcat gcctcttggg
    10621 tatggctctt gcttataatg tagccagcaa tattattacc ggctttgcta tctattattt
    10681 ctcatatgtt atcggtgatg cggatttgtt cccctattat ctgtcgtatg cgggagctgc
    10741 taacctggtg acgttagtat tcttcccacg cttagttaaa tcattatccc gacgcatttt
    10801 atgggccgga gcatctattc ttccggtgtt aagctgtggt gttctcctgt taatggcatt
    10861 aatgagctat cacaacgtcg tcctcattgt gattgcgggt attttgctga atgtgggaac
    10921 ggcgcttttc tgggtattac aggtcatcat ggtggcagat accgttgatt acggtgaata
    10981 taaactgcac gtacgctgtg aaagcatcgc ttactccgtg cagactatgg tggtgaaggg
    11041 cggttcagcc tttgcggctt ttttcattgc ggttgtgtta gggatgattg gctatgtacc
    11101 gaatgttgaa cagtctacgc aagccctatt aggtatgcag tttattatga ttgctctacc
    11161 cactctgttt ttcatggtaa cgctgattct ctacttccgt ttctatcgcc tcaatggtga
    11221 cacgctgcgc aggatccaga tccatctgct ggataaatat cgcaaagtac cgcccgagcc
    11281 tgttcatgct gatattccgg tcggtgcagt gagtgatgtg aaagcctgac gtggacaata
    11341 gctaacgaaa atggcctgat gtgttatgtt tatcaggcca cttcttaata caatgctttg
    11401 gatttgcaaa ttttgtagac cggataagga attcacgccg catccggcat caacaaagcg
    11461 cacgttgtta tccggttatc aaaccatagc gccgtagttg gcgacagtgt gttccggcga
    11521 ggagtgtcac cgtcgttaat gcgccaagca gcgcgcagaa catatcggat tgcgtgtccc
    11581 attggtcgcc ctgggtgccg agaaaatcat ccgctccctg gcccattgcc agcgctgccc
    11641 accactcgat taactcatac atcgcgctta tcgccagcgc cacgcagcag accagaaacg
    11701 ccaccatctt acgtccgcga acgtacatcc cgcgaacgag aatttctcgc gccaccagtg
    11761 caggcaccag cccctggaaa aaatgcccca gcttgtcata cggattacgg ctcaacccca
    11821 gccattcctg cacctcaaaa ccaacgggga ctttcgcgta ggtgtattgc ccgccgacca
    11881 tcaggatgat ggcgtgaaag aaaatgagcg tatagagcag cggcgttaac ggataacgtt
    11941 tagcggtggc aagcagtagc ggcacgacaa taatcaccgg tgtcacttcc atcagccagg
    12001 tgagtttgtc atgggccgaa atgccggtat aaatcaggat taacgttagc gccagtgcgc
    12061 cggtgttaag aattaacggc ttgagtgtgc gggtcatggt gagttcgcaa gtcagggaaa
    12121 atactgacta ttgaccagta agcgtgaaat tacaatcgca aaagaggtgg cccaggggga
    12181 tcacctggca gcatgctgcc aggcgctggg ccgaagaggt tacttagtgc agttcgcgca
    12241 ctgtttgttg acgatttgct ggaagaagtc gttacctttg tcatcgacca ggataaacgc
    12301 cgggaaatct tctacttcga ttttccagat agcttccata cccagctccg gataagcgac
    12361 gcattccaaa tgcttgatgc tctgctgcgc cagtaccgcc gccggaccgc cgatgctacc
    12421 gaggtagaag ccgccgtgtt tatggcacgc gtcagtaacc tgctgactgc ggttaccttt
    12481 cgccagcatg atcatgctgc cgccgtggga ttgcagcaga tccacgtagg agtccatacg
    12541 acctgcggtg gttgggccaa gtgaacctga tggataaccg gcaggggttt tcgccggacc
    12601 cgcgtagtag atcgggtgat ctttgatgta ctgcggcagt tctttaccgg cgtcaatcag
    12661 ctctttcagc ttggcgtgtg caatatcgcg acccacgata atggtgccgg tgagcgacaa
    12721 gcgggtggat accgggtatt gcgaaagctg ggcgaggatc tctttcatcg ggcggttaag
    12781 gtcaactttc accgcttcgc cttcaccggc cttgcgcagt tcttccggaa tgtactggcc
    12841 tggattgtgt tccagttttt cgatccagat accttcgcgg ttgattttcg ctttaatgtt
    12901 acggtcagcg gagcaggaga cgcccatgcc taccgggcag gatgcgccgt gacgtggcag
    12961 acggataacg cggatgtcgt gcgcgaagta tttaccgcca aactgcgcgc caagaccgag
    13021 tttctgggcc tcttccagca gttcctgttc cagctggata tcgcggaacg cctgaccatg
    13081 ttcgttccct tccgtcggca gttcatcgta atagtgagcg cttgctaact tgacggtttt
    13141 caggttggtt tccgcagacg taccgccaat cacaaacgcg atatggtacg gcgggcaggc
    13201 tgcagtaccg agggtacgca ttttctcgac gaggaagttt ttcagtttgc cgggcgtcag
    13261 cagggctttg gtttcctggt agagatacgt tttgttggca gagccgccgc ctttagagac
    13321 acagaggaat ttgtactcat cgccatctac cgcgtacagg tcgatttgcg caggcaggtt
    13381 cgtgccggtg ttgacctctt tatacatgtc cagcggcgcg ttctgtgaat agcgcaggtt
    13441 atcttcgata taggtgttat agacgccttt cgacagcgct tcttcatcac cgccaccggt
    13501 ccacacgcgc tgaccttttt taccgacgat gatcgcggtg ccggtatcct ggcaggtcgg
    13561 cagcacgcct ttggcggcga tttcggagtt tcttaagaat tgcagcgcca cgtacttgtc
    13621 gttttcgctg gcttctggat cgtgaagaat agccgcaacc tgtttctggt gtgccgggcg
    13681 gagcatgaaa gaagcatcgt gaaaggcttg ctgcgccagc agagtcaggg cttctggttc
    13741 cactttcagg atggtttcac cgtcgaagtc ggcaacgcta acgtaatcgg aagtgagtag
    13801 atagtattcg gtattgtctt tccccatcgg gaaaggtgcc tggtagataa agggtttgtt
    13861 tgacatagct tccagcctgt aactctgtat ttgttattcg aaaatggcgt gccgcgtagt
    13921 cacgggcggc acgcaaagtg catttataag aacccgtaca tcgcggcgaa gatccagccg
    13981 aagacgcacg atacgctcac accaatcaac cccggcagaa taaagctgtg gttgatgacg
    14041 aagcgaccga tgtgggtggt gccggaacgg tcaaactgaa tcgctgccag atcgctcgga
    14101 taagtcggca ggatgtaata accgtagcaa gccggtgctg aagccacgat gtatgccgga
    14161 tcaacgccga tcgccagcgc gaccggaaca atcgccgcca gcgccgcagc ctgagagttt
    14221 acaaacttgg aaaccagcag cagaacaatg gcataggccc acggatactc tttcaccatt
    14281 tcacccagta cgccctgaat ttcagacata tgcgcaccga acatggtttc agccatccat
    14341 gcgataccgt acaccgccac gatggcgatc ataccggaac ggaagacttc gttttttgag
    14401 atagacgcgg gattggtttt ggtcaggata ataatcagcg ccccggtcag cagcataaac
    14461 atctgaataa ccagtaccat cgacagcggt ttgccgccga aggatggacg caggtccgaa
    14521 tcagcaccaa gaagggcgac tacagcgatt gccccgagga agatccacat tgccagccag
    14581 ttgcttttcg gcagtttttt atcgagcagc gtcgcggtat caccgtaaac atactcacgg
    14641 ttttccggta cggagatgaa tttctggaac tcttcgtctt tatccagatc tttaccgcgg
    14701 aaccaactga agataccgat cgccaggata ccgattaacg tcgatggaat ggtgattgcc
    14761 agcaggtcga ggaactcaag atggcgacca tcaaaggtga cattacccag catcgcaacc
    14821 aaagatacga ccgcaaccga caccggactg gcgataatcc ccatctgtgc accgatagaa
    14881 cttgccgcca tcggacgttc cggacggatg ttgttcttaa tagcaacgtc gtagatgatc
    14941 ggcagaatgg tgtaaaccac atgacccgta ccgcaaagaa tggtcagcgt acaggtcaca
    15001 aacggcgcga caattgagac atatttcggg ttgcggcgca gcagcttctc ggcaatttgc
    15061 agcatgacat caagaccgcc cgaagcttgc aaggtcgccg atgctgccac caccgcaatg
    15121 ataaccagca tgacatcaac cggtggttta cctggctgaa ggtggaagac gaagaccaga
    15181 atgaccagac cgataccgcc taataaaccc agcgcgatac ccccctttct ggcaccataa
    15241 aacagacata tcagtattat gataagttgg atagtaaata acatgtgtga accctcgcga
    15301 taatcctatt taaatttttg ctgaatagat cacagtcacg ttctgttttg tatgaactgt
    15361 tttcagagcc tgactaaata tccgtttggt cgttaccggc ttgagcaaat acctcacagt
    15421 gaatattggc tgattaaagc agcggcaaat ttatgcactg tgataagcgg ctttttcaga
    15481 gagaaagact ctctggcgat gggtttatag tttgtacagc gcagatgtta atgttttgtt
    15541 tgctgttatc ttattgatat ttatcggtct gttttttgtt ctcagggcag atatcattag
    15601 gtttatagat taatctgatc tacccatttg tgggtaaaaa tacacataat gtgggtgaca
    15661 taatagttaa ttaacttttg ttagcgtttt gaaattaaaa acaccgttca tctgaagaga
    15721 tattaatttt taacgatgat ggagggataa ttatatttga tctggcacaa gttttactga
    15781 tgaacgatgt aacttgtgcc agggatattt tgcattacgg taataattat tacttacaga
    15841 taaccagcga tccgttattg gcaatattgt ttcagcagtg agtagtgttc tgcctgaata
    15901 cggtaacggt aaaccggacg ccccgtgacg ccataatgga tactggtgaa caagatgtgg
    15961 cagttgacca gccagatgag gtatttacgg catgaaacac gcgagatgtt aacctcgtta
    16021 gccagctcgt cggttgaaaa ctcatagtcc tgatgcgcgt caatccactg gcacagcgtg
    16081 cgtaacgtct gcggcgttaa gccttttggc aagcgacgag gatcctgctc gttggagctg
    16141 ctgccgtgga ttagctgatc aagctcggcc tggtcataat actgatgttt ttccagcgcc
    16201 attttctttt gccgccagcc ggtgagcgcc tcttcaaagc gggaagcctg gaagggtttg
    16261 atcaggtaat ccacaacacc gtaatgcagc gaatctttaa tggttgctgc atcggcagcg
    16321 gaggagatga caatcacatc acttttgcaa cgcgcgttat gcaggacagg cagtaaatcg
    16381 agcccgttct ctttttgcat atagatatct agcaatatca ggtcgatagg cgtgtcgctg
    16441 ttgaaaataa tctctttggc tttctccagc gtcgaagccg ttccacagca ttgaaagccc
    16501 gggatttgtg ccacgtatcg gcgattcagc tccgcgacca ttgcgtcgtc atcgataatt
    16561 aatacattga tcatctgttc gacctctccc cgtcccaggg tatctggaca aaaaattgtg
    16621 tgaaaatccc gggttccgat tccacggcga tgctgccgcc gagattttct acctgttgtt
    16681 tgacaagtgc taaaccgacg cctcgctcgc ttccttttgt cgagacacct ttgtcaaaaa
    16741 tgtgatcgat tttgtcgggt gcgatccccg gtccatcatc gttaacttca cagtgcggcc
    16801 agccgtgacg gtagtgcaat gttacgctaa tttcgcctcc gggttccggc cctaatgcct
    16861 ccagcgcgtt ttctatcaga tttcccaacg tggtaatcag cgtcgcgacc tgatcctcac
    16921 taccgctgtc tggcagctgg ctttcactgt ttaaaatcag cgtatggcct aaatcggtcg
    16981 cgcggttaat cttgctgatt aaaaaaccag cgataaccgg agatttgatc ttacccagca
    17041 gagagccaat ctcttcctga tagttattgg ctgttttgag aatgtaatct tccaactgct
    17101 tataactctt cagatgcaat aatccgagaa tcacgtgcaa tttattcata aattcgtggg
    17161 atcgttcacg aagtgcgtca gcatagttga ccagaccatc gagtcgctgc atcagtttac
    17221 gtacttcagt tttgtccctg aaggttgaaa tggcaccgat gataacgcca ttactgcgca
    17281 ccggaacggt gttgatcagt aatagccggt ctttaatcgt aatctcttcg tcgcggcgcg
    17341 gggtaccgtc gcgtaacact tccgagacat ctactacctg tgaccacgcg tggcttagcg
    17401 tcgacagctt ctcatcgtcc tgcgacttac ggtaattcag caattcttgt gcggcatcgt
    17461 tgatcagcgt gacttcgccg cgatcgtcca cggcaacgac gccttctttg atagactgca
    17521 acatggcctg gcgttgctca aacagcgtgg agatttcgta gggttccagg ccgaaaagga
    17581 tttttttcag taccttaacc agaatgcagg tgccaatcag cccgaccagc atgccaaata
    17641 ataccgacca gatgatgctc cagcgactgt cattgatctg ttgggtcacg cgacttaact
    17701 caaggccgat cgccaccacg ccaatttgtt tatgattttc atcgtagatg ggggtaaata
    17761 cgcgtaaagc ctgcgccaga aaaccgcgat tgatagcgac attttcttcg ccattcagcg
    17821 ctttaaggat gtcatcacct ttaaatggct gaccaatacg ctgggcttca ggatgcgagt
    17881 agcgaagact ttgcatatcg gtaacgacaa taaatagcag atcgttgcgt ttgcgcacgg
    17941 cttccgcgat ggcctggatg ccgctctcct gcggtttttt ctgcaagccc tgccggattt
    18001 ccggcgagtc ggcgagggta cgcgctactg ccagtgcctt gttagctagc ccgtctcgcg
    18061 tcatatcact gatttgcgag aagtaaatca gatgcaccac caatagcacc gagaacagta
    18121 ccgcgctgac cattaagatc acagtggtac tcaatttcat cggacgtttg cgtaacatac
    18181 ggtagggcaa tgaatgtctc atcagcttcc ttgtgtgaca aatttcttaa gcattatctc
    18241 tgatgaggcg ggtaattcaa agggagtaag aatgattggc tatatagggg aagagactct
    18301 ggcaacggaa actgccagtg ctgtatgaag attccggggc tatgcttata gtgaaaacca
    18361 tactgatgag agagggaagg tcatggatca ggcgctactg gacgggggtt atcgctgtta
    18421 taccggcgaa aatatcgatg tctattttaa cactgcgata tgtcagcatt ctggcaattg
    18481 cgtacgtggc aacggcaagt tatttaatct caaacgaaag ccgtggatca tgccggatga
    18541 agtcgacgtc gccactgtgg ttaaagtgat tgatacgtgc ccgagcggcg cgctgaaata
    18601 ccgtcataaa taagcgaggg taaaatggaa atacgcgaag gccacaataa attttacatt
    18661 aatgacgaac agggcaagca aatcgctgaa attgtctttg tgccgaccgg agagaattta
    18721 gcgattatcg aacataccga tgtcgatgaa agcctgaaag ggcaagggat tggtaaacag
    18781 ctggttgcga aagtcgtgga aaaaatgcgt cgggaaaaac gaaaaattat cccactatgc
    18841 ccatttgcga aacacgaatt tgataaaacg cgggagtatg atgatattcg cagttgatgg
    18901 gagagtacag agtcacaata tttttcatgc tctccgcggg gggatgcagg agagtatctg
    18961 aagggtaggg ggatgcacaa agaatggtca gagagagcgt ttttttgtcc caagtcatcc
    19021 cctttactga gcaaaaaaaa aagaatatct cctatatgag aatcatcgat cggggttaat
    19081 aagttttgcg tccccagagt gttcaatatt gataggagtc atattatgga aggtaaaaac
    19141 aagttcaata cttatgttgt ttcttttgat tatccatcat cttattcctc agtgttctta
    19201 aggttaagat cattgatgta tgatatgaat ttctcctcta tcgtggctga tgaatatggg
    19261 ataccacgac aattgaatga aaattccttc gcaataacga catcgttagc cgcaagtgaa
    19321 atcgaagatt taatcaggct taaatgctta gacttaccgg atattgattt tgacctcaac
    19381 attatgaccg ttgatgacta tttccgtcag ttttacaagt agccaggaag gataagaaga
    19441 acataaaaaa tggcactatt ctctaaaata ttaatttttt atgtgattgg tgtgaacata
    19501 tcctttgtca ttatctggtt tatctcacat gagaaaacac atattcgttt acttagcgca
    19561 ttcctggtcg gaataacctg gccaatgagt ttacctgtgg cattactttt ttctctcttt
    19621 taggagttgt agttgagtgg acccgctggt tatcttgaaa cgaagcgtct ctcctcaaaa
    19681 ctggatttgc ggaacgacga acgaagcagt cgcctgactg cttcgttcat ttaagtgaaa
    19741 tttatttctg tgggcgcatc gccgggaaga gaataacgtc gcgaatagta tggctgttag
    19801 taaacagcat aatcattcgg tcgataccaa tacccagacc agcggttggc ggcagaccat
    19861 attccagcgc agtcacgtaa tcttcgtcat agaacatggc ttcgtcgtca cctgcagctt
    19921 tagcgttaac ctgttcctgg aaacgttcag cctgatcttc tgcgtcgttt aattcgctaa
    19981 aaccattacc aatttcacga ccaccgatga agaattcaaa acggtcggtg atttccgggt
    20041 taacatcatt acggcgcgcc agcggggaca cttctgccgg atattccgta ataaaggttg
    20101 gctgaatcag atgtgcttct gccacttcat caaagatctc tgtgacaata cgtcccagcc
    20161 cccagctttt ctctaccgta attccgatag attcagctaa tgctttagca gcatcaaaat
    20221 tatccagatc cgccatatcg gtttctggac gatatttttt gattgcttcg cgcatggtga
    20281 gtttttcaaa cggtttgccg aaatcaaaca catgctcgcc ataagtgact ttagtggtac
    20341 ccagtacctc ttgtgccagg gtgcggaaca gtgactccgt cagttcaatc aaatcatggt
    20401 aatccgcata tgccatgtag agttccatca ttgtgaactc aggattatgg cgaacagaaa
    20461 taccttcatt acggaagtta cggttgattt cgaatacccg ttcaaaaccg cctacaacca
    20521 gacgtttcag atacagctcc ggcgcgatac gcaggtacat atctaaatcc agagcgttat
    20581 gatgggtaat aaacgggcga gcagatgccc cacctggaat tacctgcatc atcggggttt
    20641 ctacttccat aaagccgcgc gcgaccatga actgacggat agcggccaga atttttgaac
    20701 ggacaacaaa cgtttgacgg gatttatcgt tagcgatgag gtccagataa cgttgacgat
    20761 aacggacttc ctgatcctgc agaccatgga atttatctgg taaaggacgt agtgctttag
    20821 tcagcaggcg cagctcagta cagtgaatgg aaagctcacc cgtttgcgtc ttaaacagcg
    20881 taccgcgggc accgataatg tcacccagat cccatttttt aaactgatcg ttataaacac
    20941 cttctggcag gctatctctt gcaacgtaca gttgaatacg gccaccgaca tcctgcaacg
    21001 ttacaaagga ggctttcccc ataatacgac gggtcatcat tcggccagca accgagactt
    21061 caatgtttaa ggattccagt tcctggttat ccttcgcatc aaactcgtcg tgcaactggt
    21121 cagaggtatg gtcgcggcga aaatcattgg gaaacgccac accttgctga cgtagtgccg
    21181 ccagtttttc gcggcgattt ctcagttcat cgttaaaatc aatagcctca ttggctcccc
    21241 gtgtttctcg ttcagacatt ttggttcctc taaatccagc tttcaattta gcgtagataa
    21301 agagacagat cggtacactg tattatctgc ctcgactatt aataactcag tttatcttat
    21361 cgaaactaac gcttacctca agttgatatc actaataaat aaccataaaa atggtttaaa
    21421 tgacttatta atctcgattt actagtgtga taaactgaaa gttaatttat tcataaccaa
    21481 ttgtttttac gacagttaat cgttgctctc ctgtatcata ttcgtcggcg tgctaaacag
    21541 aaaacgggta gcaaaggcca ataccacgat gatcgcgaca caagccaacg tccactctcc
    21601 catctgagaa aagaatcgct ggtaagcggc aattgccata ccgctaattt gcgactctgt
    21661 cgtctgctgt gccacgacgc ctgccagcca gttggcgacc gcgcctgttg ccagcatata
    21721 aataccggtt aatacgccag acattttcag acgcgtgatt tgcgcaatcg ccactgggtc
    21781 aataaagagt tcagcaaagc ccattagcgc cagcccggat atcatcacgc ccattgacgc
    21841 ttgaccgcca gccgctgcat gtcgggcatc aaatgccagc aacataaagc cacaagccat
    21901 cagcagtaag ccaaaggcaa acttcagcca gacgcgcaat gctgaattgc cgcggctttc
    21961 tggcgacgcc agccaggcca gaacaacccc agcgagcatc accgcaatag cattcaccga
    22021 ctggaatagt gctgtaggta cttcaatatt gaatgcctga cgattcacaa agcgatcgat
    22081 aaacaagctg atggtactgc cgccctgttg tgccagtacc cagaacaacg tcccgacaaa
    22141 catcaacaat acaatttgcc aaagagcacg gcggtgttcg gggaatttga tcatcatgcg
    22201 ggcaatgatt tgtgcggcaa tgaggcaaac gatcgccagc aaatatccgg accagtcgtt
    22261 ctccagcagc agggtaaaaa atactggggc taaacagagc atcaccacta accaggtcca
    22321 tactggtaag gcaaatttga cgctagtgag cgctttttta tccatactac gcgtggactg
    22381 gaaatgacga tgaccactta agaaaatcaa caaaccgata aacatgccgc ctcccgcaag
    22441 ggcaaagcca acatgccatc cataccactg agcagccagg ccgcaggcga tgggggctgc
    22501 gatagaaccg atattgcccg cagcatacag cagcgaaaaa ccgccatcac gtcgatgatc
    22561 gttctcgtcg tagagctcgc caagcaaaca gctgatgttt gatttgaata aaccgtagcc
    22621 acaaataatg attgccagcg ccagatacag gctaaaggtt gaatttgtat caatgcccag
    22681 caccacatgg ccaagggtca ttaacagcgc gccggcaatc actgcagtgc ggttgccgag
    22741 caggcggtcg gcaagccagc cgccgagaat aggggtaacg taaaccagag aagcatatgc
    22801 gctgaacagg ctgatggcat ggttatcatc aaaaccaagc tgatgggtga gatagagaat
    22861 gagtaaggca cgcatgccgt aaaaactgaa gtactcccag atttggatcg ccacgatata
    22921 gtatatcgcg cgcggctgtg agggtgtttt catgtgttct ccttatgagc aaaaaaggga
    22981 agtggcaagc cacttccctt gtacgagcta attatttttt gctttcttct ttcaatacct
    23041 taacggtata gcggccatca gcctgacggt atgcaccgtg aatatcggtt tcaaagcccg
    23101 gatagtgagc gccgatttca cacagcatct gcaggaactc cagaaccgga cggctttctt
    23161 cggtgatcat ttcacccggc attaccagag gaactcccgg cggatacgga aggatcatat
    23221 tggcgttaat acgacctacc atttcgtcga ggtaaacttc ttcggtcata ccgtgcagct
    23281 ctttctggaa tgcagcatac ggagtcatta ccatcgtcgg cagcacttca aatgcgcggt
    23341 acatcagatc cggcagattg tggtgaacaa tcagtttgtg gatattctga gccagttcct
    23401 gaatacgcat gttttcatag aattcaggat cttcacgata cagagacggc agcatgtttt
    23461 tcacacgcag gttcaggtcg aacgcacgtt tgaagtcagt cagagcacgc agcaggctca
    23521 gtgctttggt cttatcgata ccgatgctga acaggaacag caggttatac ggaccggttt
    23581 tctcaacaac gatgccatgt tcgtcgaggt atttcgccac gatgctggcc ggaataccaa
    23641 agtcgctcat ggtgccgtct ttttccatcc ccggagtcag cagggtgact ttgatcgggt
    23701 caagatacat gtgctcgtta tcgatgtttt tgaagccgtg ccaggtgctg tcagaacgca
    23761 gcggccagca ttcagtcgta tcgatatgat ccggctgcca tacatcaaag aaccagccat
    23821 cagattccgt tctcagacgt ttgatctctt tacggaattt gatcgcacgt tcaatggaac
    23881 cgttgatcag acgtttacct gcattgcctt tcatcatcgc cgcagcggtt tcagtggacg
    23941 ccacgatacc gtagtgcgga gaagtggtgg tgtgcatcat gtaggcttcg ttaaaggttt
    24001 cttcgtttac gtcaccttta acgtggatca tggaagcctg agagaacgcc gccagcagtt
    24061 tgtgagtgga ctgggtttcg taaatcactt tcccttctac acggccaccg ctcataccgc
    24121 atttaccttc gtaaatcggt gagaagttgg tgtaaggcac ccacgcggag tcaaagtgga
    24181 tggatttcac atccagtgtt ttcttgatga agtcggtgtt gtacagcaga ccatcatagg
    24241 tagagttggt aattacagca tgtaccggcc aggttgcgtt tggtgtttct ttcacgcgct
    24301 tagcaatggt agcgtgctgg aattcactct gtgggatacc accaagaata ccgtaagcgt
    24361 tacgggtcgg gcggaaatag attggcgtaa catcgctcat catcatcagg tgggtcagcg
    24421 atttgtggca gttacggtca atcagaatgg tgctgcctgc cggagcagag tacatgccaa
    24481 caattttgtt cgcagtggaa gtaccgttgg tcaccatgta gctgcggtct gcgttaaaga
    24541 cgcgagcgat atactgttct gcttctttgt gtggaccact gtgatccagc agagaaccca
    24601 gttcagatac tgaaatggaa atatcagatt tcatggtatt cggaccaaag aaatcataga
    24661 acaggctacc taccgggctt ttctggaatg cagtaccgcc catgtgacca ggagtacaga
    24721 aagtatattt accttcacga acatatttaa acagtgcttt agtcagcgga ggcagaatag
    24781 tgttgatata ttcgtcagtg gtctgcttga tcttattagc aatatcttca gcagcaccca
    24841 gcgcatattc aaagaagcta atctgtaaac gcaggtcatt caggcttaca tcgagagtgg
    24901 aatacgtatt agcgaacgcg tacaacggca ggttctcgtt cattttgcta atttcttcgc
    24961 acagctcgag attatattta tcccagtcaa aaataacgcc gcacagacgc gcattgtttt
    25021 cgatcagttt taataagtcg tcacggtcgt tcgggtaaac aatctggaag ttcagacgtt
    25081 caagcgcgcg atgaagttca cggatgggtt cttctttaaa ataaaccccc atgtgattca
    25141 atattgcaat aacgttcata gtcatatctc caggtaaaaa aggcccctcc caacacatgg
    25201 gacaaaatga aaggaggagc ctcggaaaat acttttaatt aatgtgcgtt agacgctgtg
    25261 tggttatcca ttgagtggct ctggcgctcg tgcattttgc gagcgtagaa catcaggata
    25321 atcaggctga cgatgaaggt acctgccagc tcgaaggagc ttgcgcccat cagcgcgatg
    25381 aagcagaaca cgcaacccag tacagaacag atcaggctga caaagttgcg gatgttaacg
    25441 ccttcaaaac gaatcaggtc aacgcaagag tagaagtacg gcagcatagt cagcagtact
    25501 gcgataccgg tcagttcacc gaacaggtca gatgctttac caccggcaga gttcatcaga
    25561 gtgatgagga tcatcagggc agtcattttc actgcagcca gcagcagacc ttttttcgga
    25621 ataccgttgc tgtcgacttc accataaact ttcgggaagt taccgtcgtt agcggcacgt
    25681 acacctgcct ggcctaccaa catcatccag gagcccagag aagtcaggca cgcaaaggcg
    25741 gtgaatgcag aaaccagcgg cgcagcccag ttaccgagga tagttgaagc actgattgca
    25801 aacggagcac cggaagctgc cattacagaa gacggataca taccggaaag cacctgagtc
    25861 gcagcgatgt aaacaatacc tgctaaacca gtacccagca tggttgccag cggaacggta
    25921 cgtttcgggt ttttaaccat accagtactt acagctgcgg attcaacacc cacgaaggcc
    25981 cacaggcaga gcagaatact tttaatgatc gcatgaccat cggtggtatc cgcagtattc
    26041 cagttagcag cataagtcgc agcatcaaac caatgccagc caacaatagc tgtcattacc
    26101 acaggaataa gaaccagcac cagaccaata gtggttaaac ggcttaccca ggtaccgccg
    26161 agcatattta caaaggtaaa tacccagacg atagcaatac aggcgatacc cgccggaaca
    26221 ggatcattta atactgggaa gaaggtggaa agataagata cagcggtaat accaatcgcc
    26281 aggttaccaa tccagttagc atggtaataa agaacacctg tctgaaaacc aaatgcaggg
    26341 gaaatttctc cggcataagc aattgggcca ccttgttgcg ggttttttgt tgccagtcgg
    26401 gcatatacat acgccagcga cattgcacca ataatagaga taatccaacc ccagatagca
    26461 ataccaccga tacttgctag gttcgcaggt aataatgcaa taccgctccc catcatatta
    26521 ccggcaacca caccggtaca ggcaaatagc ccgatcttct tggcagaact catgctcttc
    26581 tcctaatttc atttttgaat ttggagtccg ggtcatgatg tataactatt tcctgaccag
    26641 accaaactgg cgctaagaat actcacgaaa aaaggattaa tcctaaagat taggtgaaat
    26701 aacacaaaag tttctgtaag tgagaacttg aggtttttta ttaacacatc aggatcgcaa
    26761 gttgatatca tgaaaagata aacattcaat gtttacaatg gattacggaa tattttctgg
    26821 ttaaatttat gtaataaaaa ttatgcggca aataaattgc cgcaacatat tataccaaca
    26881 ggaacataca aaaactcaac aacaaatatt cccgagcata aatcaacccg ggagttactt
    26941 attctgaagc gagaaatttg tcgagataag gtacaacata aggaacagaa gtctggaata
    27001 taccattttc aatccagtaa agggtgtttg cccctgggcg taaattaaag gcggtgagat
    27061 atgcatcagc tgcttcccgg ttcatcccct tcatttcata aaccttgcca agcaacacat
    27121 aatttagcca ggacatttca agatcaatgc cagtatttat cgcctggtaa gactcatctg
    27181 ttttaccttt taccagagca ctgaccgctt ttatttgata tataatggac aggttattca
    27241 attccggcag tgtaacaatg ttatctattt ctgtattcag tgctgctaat tgtttttcat
    27301 ctaaaggatg ttgagaatgg cgcacgatat caactaatgc tttttctgct ctcgcgtagg
    27361 taaattctgg ggatgattga acaatctcac ctaataattc actggcacga ttcaatgatt
    27421 tatcatcgcc atgcagtaaa taattatgag cctgataaaa attagttaat aacgcaccac
    27481 gatgcggcaa aatttgctgg agcgtctcct gcattcgttg tggccacggt tggtttaacg
    27541 cttttgataa actctggagt aaatcatttt gaatcgccag ctgattaccg ttagtgatga
    27601 cataacgttt atccagcatg gttgaaccat ctgcattgtc taccaatttt accgacataa
    27661 agcattgttg agcacggtat tggcgctgat taacaaacgc aatagataat gttttaccgg
    27721 aactgctcgg ttcatcaatg ttgtagttga ttttgtcatg caccataaag gtggagaagg
    27781 tgttaagtga tgtcgccacc aaatcaccca cgcctatcgc gtaagagagc tgatacgggg
    27841 aactccagct gttacaactc ttattaacca tattaatgtc aatatcgcgt ggattgagca
    27901 aaatacgcga tttgctcata ggaagacgtg tttcaagact tgaaaacgct accagtgcga
    27961 cacagatacc taacgacaac aggaaaaaaa accataccca aaaggtagtg aatcgtttgc
    28021 ttttaactgg ggattgttca ggtggcgctg tggtgttttg aatgttaaga ctgtgggagg
    28081 gagaatctgt ggcaggaacc gcctctggta tagggggagg cgaagatagc attatttcct
    28141 ctccctcttc ttcgctgtac cagataaccg gcaccattaa tttatagccg cgctttggta
    28201 cagtagcgat atagacagga ctatcttcat cattatcttt taatgactta cgtagttctg
    28261 agatactctg cgtcacaacg tgattggtga caatacttct cttccagaca ttatcgataa
    28321 gttcatccct gctaagtact tcgccactgt gttgagcaaa gaaaaccaga agatcgatta
    28381 atctcggctc aagggtaagt tgacgcccat tgcggctaat ttggtttatg gacggagtaa
    28441 caagccattc gccaacgcga actacaggtt gttgcataat agaaactcat tcgaaaaggg
    28501 aatgatgcaa tgataattgc cataatctat tttttccatc tatagatggg tttatttaca
    28561 tattattggt gaatgcaaga cgttattttt acccgccata aacttcttga ttacatagta
    28621 ttacgaaagg attttactga gaaccagaag taattttcct taccatcaaa attcatcatc
    28681 tttgccaaag aaaaatgtgc agaaaataat ccatggaaag ttgtccggag cgcttactat
    28741 tttaatggat tgttagtctt tgcatgagca agtggaccga tacatttctc tttgttctca
    28801 ttcagaaaat cttatcagtc ggcgttcaga ccgatgttgt gctttatgga tgcgttacgc
    28861 tcctgatgac gtcatttgac gttcaacagc atcacggggc cgcacgacat ttcacgtcag
    28921 tcagtgctat cgctcaggaa cacatttccc cgaattggga tatacccgca aaatgctggt
    28981 gttgtgggga gaatctggtt gagttcggta gaaatgattt ttagggagaa cgcttaaatt
    29041 gtggcgtcct gaaacgaaaa acggacctcc gtggaggtcc gtttatatga atttggtgcc
    29101 cggactcgga atgaaactgg ttatataacc aattgttttg tatcgactgt tttatccttg
    29161 ttctttttgg ggccaccaat gaggccccca tgtgcaattc gaattctttt gacgctataa
    29221 tccctctttt ttgtgctttg ttgtaatcat tttgtctggt tcgatatgct gaatcgcgga
    29281 atcgaagtaa caacaaaata cagagattta taaaatacct ggctacctgt gttagggagc
    29341 gttaacgcac agattttgtt tatggctgcc agaaatgatc tgatggtttg tcgtctggct
    29401 ctgcaagtac cctgttgata tcccgggaac catgaacgat acgttcgata cgaacttcag
    29461 tatccagtac cctgaatagc agaaggtaac gaccatacga acaacctctg atccctgccc
    29521 ctaactcggg tctttctctg tagagtactg gcgattcggc tatcaacaga cattgttggt
    29581 aaagctcttc ggtaaaactg attgcacgga caggattatc ctgagcgatc cagtcaccta
    29641 tagcttcaag atcgtgttct gctaatggtg aaacagtcag tttcatattg gctgttcacc
    29701 ttctgccatc tttttgtatt tgtgcgtcag tcgcccgaat acctcttcag cacttttact
    29761 ttcaccgctg ttgatgcctg cggtaacggc gctctggagt gattcaagct ttatctgctg
    29821 ttcccgttct tccagagcac gaagtccggc acgaatgact tcacttgtat tgttataccg
    29881 accactttct atttgttcgc ggataaacgt ttcaaagtaa ggactgaggg atacactggt
    29941 aggcatggtg attttctcgc cgattgatta ctatcaatta ataatagtta atattcgcga
    30001 attgtaaaat acctctttta tgccttgcgg cttttgttca ctgtgcttgc cagggcttcc
    30061 gagtagtgtc tggcaataat atcattaagc ctgtcaacca gttctggttt cctgaacgtg
    30121 atgtgcgcgg agcccttctt aaagtatctg atgctgaaca tctcatcttc atagtcctct
    30181 ttaccctgaa cagactggat gtggtcatcc agatgaatgg tgatgttttc ccggttgtcg
    30241 gggatcggtt tgccgctgaa cagatacagc attctttcca ggtcggcaag tcgatctgcc
    30301 tgttgcccgg tgataagatg aaacccccac cggtcccacc tcaccaggtt gttgacgata
    30361 attttactgc caaatttaca gggtgaattg ttttcgtaat tccagctcag tcccctgaaa
    30421 acattgatca ccccacgctc aaacacttca tctttgttct ggtgcagttg ttcaaatgtg
    30481 ctcagaatgt tcgcttcact gatttccgga aaattatcgt attccagtga ccggtaccat
    30541 gtatcacggg cctgcgcatc cattaatgac agcatcccgg aacgttgcat caggtcacgc
    30601 cagatttcgc gatcgagatt gcgggtgatg gctttcattg ctgtttccgg tttatccatc
    30661 agccagcagc cacagcgaaa atcctgtttc atggcccagt cccgggcggt tttacctcca
    30721 atgctttccg tcagaactga gatttcagtc agttgccgga taagtgtgtc gatttgcagc
    30781 agtgtactct ttcgtacagc cagaatgtgg cggatactgg ttgagcagat aacgtccgta
    30841 tgcccgttga ccacttccgg acgggaggct gtgatatcag acataatcga tattcctttt
    30901 tgcagataaa tgaaaacgcc agccctgttc aggctggcgt cagtggaatg aagtctggtg
    30961 agtaatattt accggttctg gtgcagaagc tgcagatgac gaagagtacg gacatcctcc
    31021 cgggcccgtt cgatacaggg caacagtgct gccgaatccg ttttatcctc atcgttgtca
    31081 aagataattc ccgattcgca gtcgatattg tcctgcagcc acacaatcag aatatccagt
    31141 gctgtttccg tggttaatga tttcatgttg ttcctggtta ccagtcgaaa gtgggcaggt
    31201 caggcaggca ttcagcgctg gcatccagat gaatgagact gacgccataa cgccggataa
    31261 gtgtgacaac cagacggcgg aacgtttttg acagtcccag gcgtttaagc cgcagaaccg
    31321 ggtatgacca cgcatcggta cgtaacagat atccgctacc tgtaaaatga atccattctg
    31381 attcgccgaa gtcattcgtc tgatgtgaca gcgagtacaa ccaggcattg tccgtttccg
    31441 tgatatgtgc ggtactgcag cgtatgccgg taagggccgc agatggtggc gtgcgctctg
    31501 ctgatgcccg gtcagattca tcaacaacac gaagggagta accgttatcg cagaccacgt
    31561 taatcagttc agcgagagta acaccatcga cgtcaacgac aatgcgcccc atgttcagtg
    31621 cctgcacgtt aatgctgtcg gcttccagcg tcagggccag tttcattgtt tcgcccccgg
    31681 atgtttaccc agggtaatgt tatttaccgt tctgtaattg tcgcgggcca tcaggccggt
    31741 tgcccggcgg gcccggagga tatcaatact gtttattaac tgagaacggg tacaggcgct
    31801 gaatcccggc tggtcggtac gtaccagtgc gtatttttca acgagaaagt tcaccgcatt
    31861 gcacagtgaa atgcctgcct caatatgctg ctcaatcaca cgttcatcag cgaacggtgt
    31921 gtcattcagt gtgaggccgt aatgctggtc caacagtcgg gtgagcagtg t
//
LOCUS       DAMZFT010000047        31865 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.047, whole genome shotgun sequence.
ACCESSION   DAMZFT010000047 DAMZFT010000000
VERSION     DAMZFT010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 31865)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31865)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31865
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.047"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            166..1218
                     /locus_tag="PN977_002249"
     CDS             166..1218
                     /locus_tag="PN977_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706977.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HDI6042278.1"
                     /translation="MPVLHNRISNDALKAKMLAESEPRTTISFYKYFHIADPKATRDA
                     LYQLFTALNVFGRVYLAHEGINAQISVPASNVETFRAQLYAFDPALEGLRLNIALEDD
                     GKSFWVLRMKVRDRIVADGIDDPHFDASNVGEYLQAAEVNAMLDDPDALFIDMRNHYE
                     YEVGHFENALEIPADTFREQLPKAVEMMQAHKDKKIVMYCTGGIRCEKASAWMKHNGF
                     NKVWHIEGGIIEYARKAREQGLPVRFIGKNFVFDERMGERISDEIIAHCHQCGAPCDS
                     HTNCKNDGCHLLFIQCPVCAEKYKGCCSEICCEESALPPEEQRRRRAGRENGNKIFNK
                     SRGRLNTTLGIPDPTE"
     gene            complement(1260..1835)
                     /locus_tag="PN977_002250"
     CDS             complement(1260..1835)
                     /locus_tag="PN977_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HDI6042279.1"
                     /translation="MKKSLLGLTFASLMFSAGSAVAADYKIDKEGQHAFVNFRIQHLG
                     YSWLYGTFKDFDGTFTFDEKNPAADKVNVTINTTSVDTNHAERDKHLRSADFLNTAKY
                     PQATFTSTSVKKDGDELDITGDLTLNGVTKPVTLEAKLIGQGDDPWGGKRAGFEAEGK
                     IKLKDFNIKTDLGPASQEVDLIISVEGVQQK"
     gene            complement(1839..2405)
                     /locus_tag="PN977_002251"
     CDS             complement(1839..2405)
                     /locus_tag="PN977_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HDI6042280.1"
                     /translation="MSFTNTPERYGVISAALHWISAIIVYGMFALGLWMVTLSYYDGW
                     YHKAPELHKSIGILLMMGLVIRVLWRVISPPPGPLQSYSSMTRLGAKAGHLALYLLLF
                     AIGISGYLISTADGKPISVFGWFDVPATLADAGAQADFAGALHFWLAWSVVVLSVMHG
                     FMALKHHFIDKDDTLKRMLGKSSSDYGV"
     gene            complement(2666..2779)
                     /locus_tag="PN977_002252"
     CDS             complement(2666..2779)
                     /locus_tag="PN977_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceO family protein"
                     /protein_id="HDI6042281.1"
                     /translation="MRRLLHYLINNIREHLMLYLFLWGLLAIMDLIYVFYF"
     gene            complement(2827..3945)
                     /gene="solA"
                     /locus_tag="PN977_002253"
     CDS             complement(2827..3945)
                     /gene="solA"
                     /locus_tag="PN977_002253"
                     /EC_number="1.5.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-methyl-L-tryptophan oxidase"
                     /protein_id="HDI6042282.1"
                     /translation="MKYDLIIIGSGSVGAAAGYYATRAGLNVLMTDAHMPPHQHGSHH
                     GDTRLIRHAYGEGEKYVPLVLRAQMLWDELSRHNEDDPIFVRSGVINLGPADSAFLAN
                     VAHSAEQWQLNVEQLDAQGIMARWPEIRVPDNYIGLFETDSGFLRSELAIKTWIQLAK
                     EAGCAQLFNCPVTEIRHDDDGVTIETADGEYQAKKAIVCAGTWVKDLLPELPVQPVRK
                     VFAWYQADGRYSVKNKFPAFTGELPNGDQYYGFPAENDALKIGKHNGGQVIHSADERV
                     PFAEVVSDGSEAFPFLRNVLPGIGCCLYGAACTYDNSPDEDFIIDTLPAHDNTLLITG
                     LSGHGFKFASVLGEIAADFAQDKKSDFDLTPFRLSRFQ"
     gene            complement(4060..4314)
                     /gene="bssS"
                     /locus_tag="PN977_002254"
     CDS             complement(4060..4314)
                     /gene="bssS"
                     /locus_tag="PN977_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309465.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssS"
                     /protein_id="HDI6042283.1"
                     /translation="MEKNNEVIQTHPLVGWDISTVDSYDALMLRLHYQTPNKSEQEGT
                     EVGQTLWLTTDVARQFISILEAGIAKIESGDFPVNEYRRH"
     gene            complement(4604..4849)
                     /gene="dinI"
                     /locus_tag="PN977_002255"
     CDS             complement(4604..4849)
                     /gene="dinI"
                     /locus_tag="PN977_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein I"
                     /protein_id="HDI6042284.1"
                     /translation="MRIEVTIAKTSPLPAGAIDALAGELSRRIQYAFPDNEGHVSVRY
                     AAANNLSVIGATKEDKQRISEILQETWESADDWFVSE"
     gene            complement(4923..5969)
                     /gene="pyrC"
                     /locus_tag="PN977_002256"
     CDS             complement(4923..5969)
                     /gene="pyrC"
                     /locus_tag="PN977_002256"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HDI6042285.1"
                     /translation="MTAPSQVLKIRRPDDWHLHLRDGDMLKTVVPYTSEIYGRAIVMP
                     NLAPPVTTVEAAVAYRQRILDAVPAGHDFTPLMTCYLTDSLDPNELERGFNEGVFTAA
                     KLYPANATTNSSHGVTSVDAIMPVLERMEKIGMPLLVHGEVTHADIDIFDREARFIES
                     VMEPLRQRLTALKVVFEHITTKDAADYVRDGNERLAATITPQHLMFNRNHMLVGGVRP
                     HLYCLPILKRNIHQQALRELVASGFNRVFLGTDSAPHARHRKESSCGCAGCFNAPTAL
                     GSYATVFEEMNALQHFEAFCSVNGPQFYGLPVNDTFIELVREEQQVAESIALTDDTLV
                     PFLAGETVRWSVKQ"
     gene            complement(6075..6635)
                     /locus_tag="PN977_002257"
     CDS             complement(6075..6635)
                     /locus_tag="PN977_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HDI6042286.1"
                     /translation="MNKFLFAAALIVSGLLVGCNQLTQYTITEQEINQSLAKHNNFSK
                     DIGLPGVADAHIVLTNLTSQIGREEPNKVTLTGDANLDMNSLFGSQKATMKLKLKALP
                     VFDKEKGAIFLKEIEVVDATVQPEKMQTVMQTLLPYLNQALRNYFNQQPAYVLREDGS
                     QGEAMAKKLAKGIEVKPGEIVIPFTD"
     gene            complement(6769..7416)
                     /gene="grxB"
                     /locus_tag="PN977_002258"
     CDS             complement(6769..7416)
                     /gene="grxB"
                     /locus_tag="PN977_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 2"
                     /protein_id="HDI6042287.1"
                     /translation="MKLYIYDHCPYCLKARMIFGLKNIPVELHVLLNDDAETPTRMVG
                     QKQVPILQKDDSRYMPESMDIVHYVDKLDGKPLLTGKRSPAIEEWLRKVNGYANKLLL
                     PRFAKSAFDEFSTPAARKYFVDKKEASAGNFADLLAHSDGLIKNISDDLRALDKLIVK
                     PNAVNGELSEDDIQLFPLLRNLTLVAGINWPSRVADYRDNMAKQTQINLLSSMAI"
     gene            complement(7480..8688)
                     /gene="mdtH"
                     /locus_tag="PN977_002259"
     CDS             complement(7480..8688)
                     /gene="mdtH"
                     /locus_tag="PN977_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtH"
                     /protein_id="HDI6042288.1"
                     /translation="MSRVSQARNLGKYFLLIDNMLVVLGFFVVFPLISIRFVDQMGWA
                     AVMVGIALGLRQFIQQGLGIFGGAIADRFGAKPMIVTGMLMRAAGFATMGIAHEPWLL
                     WFSCLLSGLGGTLFDPPRSALVVKLIRPQQRGRFFSLLMMQDSAGAVIGALLGSWLLQ
                     YDFRLVCATGAVLFVLCAAFNAWLLPAWKLSTVRTPVREGMTRVMRDKRFVTYVLTLA
                     GYYMLAVQVMLMLPIMVNDVAGAPSAVKWMYAIEACLSLTLLYPIARWSEKHFRLEHR
                     LMAGLLIMSLSMMPVGMVSGLQQLFTLICLFYIGSIIAEPARETLSASLADARARGSY
                     MGFSRLGLAIGGAIGYIGGGWLFDLGKSAHQPELPWMMLGIIGIFTFLALGWQFSQKR
                     TARRLLERDA"
     gene            8924..9508
                     /gene="rimJ"
                     /locus_tag="PN977_002260"
     CDS             8924..9508
                     /gene="rimJ"
                     /locus_tag="PN977_002260"
                     /EC_number="2.3.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S5-alanine
                     N-acetyltransferase"
                     /protein_id="HDI6042289.1"
                     /translation="MFGYRSNVPKVRLTTDRLVVRLVHDRDAWRLADYYAENRHFLKP
                     WEPVRDESHCYPSGWQARLGMINEFHKQGSAFYFGLFDPDEKEIIGVANFSNVVRGSF
                     HACYLGYSIGQKWQGKGLMFEALTAAIRYMQRTQHIHRIMANYMPHNKRSGDLLARLG
                     FEKEGYAKDYLLIDGQWRDHVLTALTTPDWTPGR"
     gene            9519..10166
                     /locus_tag="PN977_002261"
     CDS             9519..10166
                     /locus_tag="PN977_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceH family protein"
                     /protein_id="HDI6042290.1"
                     /translation="MKYQLTALEARVIGCLLEKQVTTPEQYPLSVNGVVTACNQKTNR
                     EPVMNLSESEVQEQLDNLVKRHYLRTVSGFGNRVTKYEQRFCNSEFGDLKLSAAEVAL
                     ITTLLLRGAQTPGELRSRAARMYEFSDMAEVESTLEQLANREDGPFVVRLAREPGKRE
                     SRYMHLFSGEVEDQPAVMDMSNAVDGDLQARVEALEIEVAELKQRLDSLLAHLGD"
     gene            10168..11091
                     /locus_tag="PN977_002262"
     CDS             10168..11091
                     /locus_tag="PN977_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HDI6042291.1"
                     /translation="MKKLRIGVVGLGGIAQKAWLPVLAAASDWTLQGAWSPTRAKALP
                     ICDSWRIPYADSLSSLAASCDAVFVHSSTASHFDVVSTLLNAGGHVCVDKPLAENLRD
                     AERLVELAARKKLTLMVGFNRRFAPLYGELKTQLATAASLRMDKHRSNSVGPHDLYFT
                     LLDDYLHVVDTALWLSGGKASLDGGTLLTNDAGEMLFAEHHFSAGPLQITTCMHRRAG
                     SQRETVQAVTDGALIDITDMREWREERGQGVVHKPILGWQSTLEQRGFVGCARHFIEC
                     VQNQTVPQTAGEQAVLAQRIVDKIWRDAMSE"
     gene            11201..12736
                     /gene="murJ"
                     /locus_tag="PN977_002263"
     CDS             11201..12736
                     /gene="murJ"
                     /locus_tag="PN977_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706990.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HDI6042292.1"
                     /translation="MNLLKSLAAVSSMTMFSRVLGFARDAIVARIFGAGMATDAFFVA
                     FKLPNLLRRIFAEGAFSQAFVPILAEYKSKQGEDATRVFVSYVSGLLTLALAVVTVAG
                     MLAAPWVIMVTAPGFADTADKFALTSQLLKITFPYILLISLASLVGAILNTWNRFSIP
                     AFAPTLLNISMIGFALFAAPYFNPPVLALAWAVTVGGILQLVYQLPHLKKIGMLVLPR
                     INFHDAGAMRVVKQMGPAILGVSVSQISLIINTIFASFLASGSVSWMYYADRLMEFPS
                     GVLGVALGTILLPSLSKSFASGNHDEYNRLMDWGLRLCFLLALPSAVALGILSGPLTV
                     SLFQYGKFTAFDALMTQRALIAYSVGLIGLIVVKVLAPGFYSRQDIKTPVKIAIVTLI
                     LTQLMNLAFIGPLKHAGLSLSIGLAACLNASLLYWQLRKQKIFTPQPGWMAFLLRLVV
                     AVLVMSGVLLGMLHIMPEWSLGTMPWRLLRLMAVVLAGIAAYFAALAVLGFKVKEFAR
                     RTV"
     gene            complement(12776..13192)
                     /gene="flgN"
                     /locus_tag="PN977_002264"
     CDS             complement(12776..13192)
                     /gene="flgN"
                     /locus_tag="PN977_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis chaperone FlgN"
                     /protein_id="HDI6042293.1"
                     /translation="MTRLAEILDQMSAVLNDLKTVMDQEQQHLSMGQINGSQLQWITE
                     QKSSLLATLDYLEQLRRKEPNSANSVDISQRWQEITVKTQQLRQMNQHNGWLLEGQIE
                     RNQQALEMLKPHQEPTLYGANGQTSTTHRGSKKISI"
     gene            complement(13197..13490)
                     /gene="flgM"
                     /locus_tag="PN977_002265"
     CDS             complement(13197..13490)
                     /gene="flgM"
                     /locus_tag="PN977_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma-28 factor FlgM"
                     /protein_id="HDI6042294.1"
                     /translation="MSIDRTSPLKPVSTVQPRETTDAPVTNTRAAKTTAATSTSVTLS
                     DAQAKLMQPGSSDINPERVEALKLAIRNGELKMDTGKIADALINEAQQDLQSN"
     gene            complement(13566..14225)
                     /gene="flgA"
                     /locus_tag="PN977_002266"
     CDS             complement(13566..14225)
                     /gene="flgA"
                     /locus_tag="PN977_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring formation protein
                     FlgA"
                     /protein_id="HDI6042295.1"
                     /translation="MLAIKRRVAIIAILFSPLSAASNLTSQLHTFFSAQLAGVSDEVR
                     VSIRTAPNLLPPCEQPLLSMSNNSRLWGNVNVLARCGNDKRYLQVNVQATGNYVVAAM
                     PIVRGGKLEAGNVKLKRGRLDTLPPRTVLDINQLVDAVSLRDISPDQPIQLTQFRQAW
                     RVKAGQRVNVIASGDGFSANAEGQALNNAAVAQNARVRMVSGQVVSGVVDADGNILIN
                     L"
     gene            14381..14797
                     /gene="flgB"
                     /locus_tag="PN977_002267"
     CDS             14381..14797
                     /gene="flgB"
                     /locus_tag="PN977_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HDI6042296.1"
                     /translation="MLDKLDAALRFQQEALNLRAQRQEVLAANIANADTPGYQARDID
                     FASELKKVMQRGRDATSVVALTMTSTQHIPAQALTPPTAELQYRIPDQPSLDGNTVDM
                     DRERTQFADNSLQYQMSLSALSGQIKGMMNVLQSGN"
     gene            14801..15205
                     /gene="flgC"
                     /locus_tag="PN977_002268"
     CDS             14801..15205
                     /gene="flgC"
                     /locus_tag="PN977_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HDI6042297.1"
                     /translation="MALLNIFDIAGSALTAQSQRLNVAASNLANADSLTGPDGQPYRA
                     KQVVFQVNAAPGAATGGVKVADVIESQAPDKLVYEPGNPLADAKGYVKMPNVDVVGEM
                     VNTMSASRSYQANVEVLNTVKSMMLKTLTLGQ"
     gene            15217..15912
                     /gene="flgD"
                     /locus_tag="PN977_002269"
     CDS             15217..15912
                     /gene="flgD"
                     /locus_tag="PN977_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook assembly protein FlgD"
                     /protein_id="HDI6042298.1"
                     /translation="MSIAVTTTDPTNTGVSTTSSSSLTGSNAADLQSSFLTLLVAQLK
                     NQDPTNPMENNELTSQLAQISTVSGIEKLNTTLGSISGQIDNSQSLQASNLIGHGVMI
                     PGTTVLAGTGSDEGAVTTTTPFGVELQQAADKVTATITDKNGAVVRTIDIGELTAGVH
                     SFTWDGTLTDGSTAPNGSYNVAISASSGGTQLVAQPLQFALVQGVIRGNNGNTLDLGT
                     YGTTTLDEVRQII"
     gene            15937..17142
                     /gene="flgE"
                     /locus_tag="PN977_002270"
     CDS             15937..17142
                     /gene="flgE"
                     /locus_tag="PN977_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook protein FlgE"
                     /protein_id="HDI6042299.1"
                     /translation="MAFSQAVSGLNAAATNLDVIGNNIANSATYGFKSGTASFADMFA
                     GSKVGLGVKVAGITQDFTDGTTTNTGRGLDVAISQNGFFRLVDSNGSVFYSRNGQFKL
                     DENRNLVNMQGLQLTGYPATGTPPTIQQGANPTNISIPNTLMAAKTTTTASMQINLNS
                     SDPLPSVKAFDASNADSYNKKGSVTVFDSQGNAHDMSVYFVKTGDNNWDVYTLDSSDP
                     TGTANPATTLVFNANGVLTSDPTKDITTGAINGADPATFSLSFLNSMQQNTGANNIVA
                     TTQNGYKPGDLVSYQINDDGTVVGNYSNEQTQLLGQIVLANFANNEGLASEGDNVWSA
                     TQSSGVALLGTAGTGNFGTLTNGALEASNVDLSKELVNMIVAQRNYQSNAQTIKTQDQ
                     ILNTLVNLR"
     gene            17162..17917
                     /gene="flgF"
                     /locus_tag="PN977_002271"
     CDS             17162..17917
                     /gene="flgF"
                     /locus_tag="PN977_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgF"
                     /protein_id="HDI6042300.1"
                     /translation="MDHAIYTAMGAASQTLNQQAVTASNLANASTPGFRAQLNALRAV
                     PVEGLSLPTRTLVTASTPGADMTPGKMDYTSRPLDVALQQDGWLAVQTADGSEGYTRN
                     GSIQVDPTGQLTIQGHPVIGEAGPIAVPEGAEITIAADGTISALNPGDPANTVAPVGR
                     LKLVKATGSEVQRGDDGIFRLSAESQATRGPVLQADPTLRVMSGVLEGSNVNAVAAMS
                     DMIASARRFEMQMKVISSVDDNAGRANQLLSMS"
     gene            18054..18836
                     /gene="flgG"
                     /locus_tag="PN977_002272"
     CDS             18054..18836
                     /gene="flgG"
                     /locus_tag="PN977_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
                     /protein_id="HDI6042301.1"
                     /translation="MISSLWIAKTGLDAQQTNMDVIANNLANVSTNGFKRQRAVFEDL
                     LYQTIRQPGAQSSEQTTLPSGLQIGTGVRPVATERLHSQGNLSQTNNSKDVAIKGQGF
                     FQVMLPDGSSAYTRDGSFQVDQNGQLVTAGGFQVQPAITIPANALSITIGRDGVVSVT
                     QQGQAAPVQVGQLNLTTFMNDTGLESIGENLYTETQSSGAPNESTPGLNGAGLLYQGY
                     VETSNVNVAEELVNMIQVQRAYEINSKAVSTIDQMLQKLTQL"
     gene            18889..19587
                     /gene="flgH"
                     /locus_tag="PN977_002273"
     CDS             18889..19587
                     /gene="flgH"
                     /locus_tag="PN977_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HDI6042302.1"
                     /translation="MQKNAAHTYAISSLLVLSLTGCAWIPSTPLVQGATSAQPVPGPT
                     PVANGSIFQSAQPINYGYQPLFEDRRPRNIGDTLTIVLQENVSASKSSSANASRDGKS
                     NFGFDTVPRYLQGLFGNARADVEASGGNTFNGKGGANASNTFSGTLTVTVDQVLVNGN
                     LHVVGEKQIAINQGTEFIRFSGVVNPRTISGSNTVPSTQVADARIEYVGNGYINEAQN
                     MGWLQRFFLNLSPM"
     gene            19599..20696
                     /gene="flgI"
                     /locus_tag="PN977_002274"
     CDS             19599..20696
                     /gene="flgI"
                     /locus_tag="PN977_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415598.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HDI6042303.1"
                     /translation="MIKFLSALILLLVTTAVQAERIRDLTSVQGVRQNSLIGYGLVVG
                     LDGTGDQTTQTPFTTQTLNNMLSQLGITVPTGTNMQLKNVAAVMVTASLPPFGRQGQT
                     IDVVVSSMGNAKSLRGGTLLMTPLKGVDSQVYALAQGNILVGGAGASAGGSSVQVNQL
                     NGGRITNGAVIERELPSQFGVGNTLNLQLNDEDFSMAQQIADTINRVRGYGSATALDA
                     RTIQVRVPSGNSSQVRFLADIQNMQVNVTPQDAKVVINSRTGSVVMNREVTLDSCAVA
                     QGNLSVTVNRQANVSQPDTPFGGGQTVVTPQTQIDLRQSGGSLQSVRSSASLNNVVRA
                     LNALGATPMDLMSILQSMQSAGCLRAKLEII"
     gene            20696..21637
                     /gene="flgJ"
                     /locus_tag="PN977_002275"
     CDS             20696..21637
                     /gene="flgJ"
                     /locus_tag="PN977_002275"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly peptidoglycan hydrolase FlgJ"
                     /protein_id="HDI6042304.1"
                     /translation="MISDSKLLASAAWDAQSLNELKAKAGEDPAANIRPVARQVEGMF
                     VQMMLKSMRDALPKDGLFSSEHTRLYTSMYDQQIAQQMTAGKGLGLAEMMVKQMTPEQ
                     PLPEESMPAAPMKFPLETVVRYQNQTLSQLVQKAVPRNYDDSLPGDSKAFLAQLSLPA
                     QLASQQSGVPHHLILAQAALESGWGQRQIRRENGEPSYNLFGVKASGNWKGPVTEITT
                     TEYENGEAKKVKAKFRVYSSYLEALSDYVGLLTRNPRYAAVTTAASAEQGAQALQDAG
                     YATDPHYARKLTSMIQQMKSISDKVSKTYSMNIDNLF"
     gene            21703..23346
                     /gene="flgK"
                     /locus_tag="PN977_002276"
     CDS             21703..23346
                     /gene="flgK"
                     /locus_tag="PN977_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HDI6042305.1"
                     /translation="MSSLINNAMSGLNAAQAALNTASNNISSYNVAGYTRQTTIMAQA
                     NSTLGAGGWVGNGVYVSGVQREYDAFITNQLRAAQTQSSGLTARYEQMSKIDNMLSTS
                     TSSLATQMQDFFTSLQTLVSNAEDPAARQALIGKSEGLVNQFKTTDQYLRDQDKQVNI
                     AIGASVDQINNYAKQIASLNDQISRLTGVGAGASPNNLLDQRDQLVSELNQIVGVEVS
                     VQDGGTYNITMANGYSLVQGSTARQLAAVPSSADPSRTTVAYIDGTAGNIEIPEKLLN
                     TGSLGGILTFRSQDLDQTRNTLGQLALAFAEAFNTQHKAGFDANGDAGEDFFAIGKPA
                     VLQNTKNKGDVAIGATVTDASAVLATDYKISFDNNQWQATRLASNTTFTVTPDANGKV
                     AFDGLELTFTGTPAVNDSFTLKPVSDAIVNMDVLITDEAKIAMASEEDVGDSDNRNGQ
                     ALLDLQSNSKTVGGAKSFNDAYASLVSDIGNKTATLKTSSATQGNVVTQLSNQQQSIS
                     GVNLDEEYGNLQRFQQYYLANAQVLQTANAIFDALINIR"
     gene            23358..24311
                     /gene="flgL"
                     /locus_tag="PN977_002277"
     CDS             23358..24311
                     /gene="flgL"
                     /locus_tag="PN977_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgL"
                     /protein_id="HDI6042306.1"
                     /translation="MRFSTQMMYQQNMRGITNSQAEWMKYGEQMSTGKRVVNPSDDPI
                     AASQAVVLSQAQAQNSQYTLARTFATQKVSLEESVLSQVTTAIQNAQEKIVYASNGTL
                     SDDDRASLATDIQGLRDQLLNLANTTDGNGRYIFAGYKTESAPFSEVDGKYEGGAESI
                     KQQVDASRSMVIGHTGDKIFDSITSNAVAEPDGSASETNLFAMLDSAIAALKTPVADS
                     EADKETAAAALDKTNRGLKNSLNNVLTVRAELGTQLNELESLDSLGSDRALGQTQQMS
                     DLVDVDWNATISSYIMQQTALQASYKAFTDMQGLSLFQLNK"
     gene            complement(24406..24900)
                     /locus_tag="PN977_002278"
     CDS             complement(24406..24900)
                     /locus_tag="PN977_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000613627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDI6042307.1"
                     /translation="MIQAPEPLMARHQFTSFCSGVETMDNWLKQRALKNQLAGASRTF
                     VSCDTYSNVLAYYSLASSAVETYVATGRFRRNMPEPIPVVVLGRLAIDKSLQGQGIGR
                     AMVRDAGLRVLQAAEVIGIRGMLVHALSDQAREFYLRVGFEPSPVDSMILMATLADLQ
                     ECLK"
     gene            complement(24897..25190)
                     /locus_tag="PN977_002279"
     CDS             complement(24897..25190)
                     /locus_tag="PN977_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HDI6042308.1"
                     /translation="MPAANNIPARRESLNLRIKPAERDLIDRAAKARGKNRTEFVLEA
                     ARAAAEEALIEQRIFRIDSDDYQLFLNRLDQPPAPNAALRKTMQTPAPWEQKK"
     gene            complement(25324..28497)
                     /gene="rne"
                     /locus_tag="PN977_002280"
     CDS             complement(25324..28497)
                     /gene="rne"
                     /locus_tag="PN977_002280"
                     /EC_number="3.1.26.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E"
                     /protein_id="HDI6042309.1"
                     /translation="MKRMLINATQQEELRVALVDGQRLYDLDIESPGHEQKKANIYKG
                     KITRIEPSLEAAFVDYGAERHGFLPLKEIAREYFPANYSAHGRPNIKDVLREGQEVIV
                     QIDKEERGNKGAALTTFISLAGSYLVLMPNNPRAGGISRRIEGDDRTELKEALASLEL
                     PEGMGLIVRTAGVGKSAEALQWDLSFRLKHWEAIKKAAESRPAPFLIHQESNVIVRAF
                     RDYLRQDIGEILIDNPKVLELARQHIAALGRPDFSSKIKLYTGEIPLFSHYQIESQIE
                     SAFQREVRLPSGGSIVIDSTEALTAIDINSARATRGGDIEETAFNTNLEAADEIARQL
                     RLRDLGGLIVIDFIDMTPVRHQRAVENRLREAVRQDRARIQISHISRFGLLEMSRQRL
                     SPSLGESSHHVCPRCSGTGTVRDNESLSLSILRLIEEEALKENTQEVHAIVPVPIASY
                     LLNEKRSAVNAIETRQDGVRCVIVPNDQMETPHYHVLRVRKGEETPTLSYMLPKLHEE
                     AMALPSEEEFAERKRPEQPALATFAMPDVPPAPTPAEPAAPVVAPAPKAATATPASPA
                     QPGLLSRFFGALKALFSGGEETKPAEQPAPKAEAKPERQQDRRKPRQNNRRDRNERRD
                     SRSERTEGSDNREENRRNRRQAQQQTVETRESRQQAEVTEKARTTDEQQAPRRERSRR
                     RNDDKRQAQQEAKALNVEEQSVQETEQEERVRPVQPRRKQRQLNQKVRYEQSVAEEAV
                     VAPVVEETVAGEPIVQEAPAPRTELVKVPLPVVAQAAPEQQEENNADNRDNGGMPRRS
                     RRSPRHLRVSGQRRRRYRDERYPTQSPMPLTVACASPELASGKVWIRYPIVRPQDVQV
                     EEQREQEEVQVQPMVTEVPVAAAVEPVVSAPVVEEVAEVVEAPVHVAEPQPEVVETTH
                     PEVIAAAVTEQPQVITESDVAVAQEVAEHAEPVVEPQEETADIEEVAETAEVVVAEPE
                     VVAQPAAPVAAEVETVAAVEPEITVEHNHATAPMTRAPAPEYVPEAPRHSDWQRPTFA
                     FEGKGAAGGHTATHHASAAPARPQPVE"
     gene            29070..30029
                     /gene="rluC"
                     /locus_tag="PN977_002281"
     CDS             29070..30029
                     /gene="rluC"
                     /locus_tag="PN977_002281"
                     /EC_number="5.4.99.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(955/2504/2580) synthase
                     RluC"
                     /protein_id="HDI6042310.1"
                     /translation="MKTETPSVKIVAITADEAGQRIDNFLRTQLKGVPKSMIYRILRK
                     GEVRVNKKRIKPEYKLEAGDEVRIPPVRVAEREEEAVSPHLQKVAALADVILYEDDHI
                     LVLNKPSGTAVHGGSGLSFGVIEGLRALRPEARFLELVHRLDRDTSGVLLVAKKRSAL
                     RSLHEQLREKGMQKDYLALVRGQWQSHVKSVQAPLLKNILQSGERIVRVSQEGKPSET
                     RFKVEERYAFATLVRCSPVTGRTHQIRVHTQYAGHPIAFDDRYGDREFDRHLTEAGTG
                     LNRLFLHAAALKFTHPGTGEVMRIEAPMDEGLKRCLQKLRNAR"
     gene            complement(30140..30724)
                     /gene="yceF"
                     /locus_tag="PN977_002282"
     CDS             complement(30140..30724)
                     /gene="yceF"
                     /locus_tag="PN977_002282"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-methyl-GTP pyrophosphatase"
                     /protein_id="HDI6042311.1"
                     /translation="MPKLILASTSPWRRALLEKLQISFECAAPEVDETPRSDESPRQL
                     VLRLAQEKAQSLASRYPDHLIIGSDQVCVLDGEITGKPLTEENARLQLRKASGNIVTF
                     DTGLALFNSANGHLQTEVEPFDVHFRHLSEAEIDNYVRKEHPLHCAGSFKSEGFGITL
                     FERLEGRDPNTLVGLPLIALCQMLRREGKNPLMG"
     gene            30923..31444
                     /gene="yceD"
                     /locus_tag="PN977_002283"
     CDS             30923..31444
                     /gene="yceD"
                     /locus_tag="PN977_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001174481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA accumulation protein YceD"
                     /protein_id="HDI6042312.1"
                     /translation="MQKVKLPLTLDPVRTAQKRLDYQGIYTPDQVERVAESVVSVDSD
                     VECSMSFAIDNQRLAVLNGDAKVTVTLECQRCGKPFTHQVYTTYCFSPVRSDEQAEAL
                     PEAYEPIEVNEFGEIDLLAMVEDEIILALPVVPVHDSEHCEVSEADMVFGELPEEAQK
                     PNPFAVLASLKRK"
     gene            31496..31669
                     /gene="rpmF"
                     /locus_tag="PN977_002284"
     CDS             31496..31669
                     /gene="rpmF"
                     /locus_tag="PN977_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HDI6042313.1"
                     /translation="MAVQQNKPTRSKRGMRRSHDALTAVTSLSVDKTSGEKHLRHHIT
                     ADGYYRGRKVIAK"
     gene            31750..>31865
                     /locus_tag="PN977_002285"
     CDS             31750..>31865
                     /locus_tag="PN977_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase"
                     /protein_id="HDI6042314.1"
                     /translation="MTRLTLALDVMGGDFGPSVTVPAALQALNSNSQLTLLLV"
BASE COUNT         7769 a         8009 c         8416 g         7671 t
ORIGIN      
        1 gcaaccaaaa tttgtgactg aagactgggc gaaattgccg cgattgtaaa taacaaataa
       61 tttttaatgc gcaaatgtag cgtaaaatgt gtggatgtta attatcgata attgctatat
      121 catgccgcgg atttttactt tcccatctcg caggaaccgt acaccatgcc agtgttacac
      181 aaccgcattt ccaacgacgc gttaaaagcc aaaatgttgg ctgagagcga accgcgaacc
      241 accatttcgt tttataagta tttccacatc gccgatccta aggcgacccg tgacgcttta
      301 tatcagctgt ttaccgcgct gaatgttttt gggcgagtgt atctggcgca tgagggcatt
      361 aacgcgcaaa tcagcgtacc tgcgagcaat gttgaaacat ttcgcgcgca gctttatgcc
      421 ttcgacccgg ctttagaggg cttacgcctg aatatcgcgt tggaagatga cgggaaatcc
      481 ttctgggtac tgcgcatgaa ggtccgcgat cgtatcgttg ccgacggtat tgacgatcct
      541 cactttgatg ccagcaatgt gggtgagtat ctgcaagcgg cggaagtgaa cgccatgctt
      601 gacgatcccg atgcattgtt tatcgacatg cgtaaccact atgagtatga agtggggcac
      661 tttgaaaacg cgctggaaat tccggcagat accttccgtg agcagctgcc aaaagcagtt
      721 gagatgatgc aggcacataa agataaaaaa atcgtcatgt actgcaccgg cggcattcgt
      781 tgtgaaaaag ccagtgcctg gatgaaacat aacggattca ataaagtctg gcatatcgag
      841 ggtggaatta ttgaatacgc ccgtaaggcg cgcgagcagg gcttgccggt gcgttttatt
      901 ggcaaaaatt ttgtttttga cgagcggatg ggcgaacgta tatctgatga gattatcgcg
      961 cattgccacc agtgcggtgc gccgtgcgac agccatacca actgtaaaaa tgatggctgc
     1021 catctgcttt ttattcagtg tccagtatgt gcggaaaaat acaaaggttg ttgtagtgag
     1081 atttgctgcg aagaaagcgc gttaccgcca gaggaacagc gacgccgtcg ggcaggacgt
     1141 gaaaatggca ataagatctt taataagtct cgtggacgtc tgaatacaac actgggcatt
     1201 cctgatccaa cagagtaaat atcattgccg gatgcgtgcc atccggcaac atttcactct
     1261 tacttctgct gtacgccttc cactgaaata atcagatcca cttcctgaga agctggacct
     1321 aaatctgttt tgatattgaa gtccttgagc ttaattttgc cttcggcctc gaagcctgca
     1381 cgtttaccac cccacgggtc gtcgccttga ccaattaatt tcgcttccag cgtgacgggt
     1441 ttggttacgc cattcagcgt cagatcgccg gtaatatcca gttcgtcacc gtctttcttc
     1501 acgctggtgg aggtgaatgt tgcctgtgga tattttgcag tattgaggaa atctgcactg
     1561 cgaagatgtt tatcgcgttc ggcgtgatta gtatcgacgc tggtggtgtt aattgtcaca
     1621 ttcactttat cggcagccgg atttttttca tcaaaggtaa aagtaccgtc gaaatcttta
     1681 aaggtgccat ataaccagct atagccaaga tgctggatgc ggaagttaac aaaggcgtgc
     1741 tgaccttctt tgtcaatttt gtaatcggcg gcaaccgctg aaccggcaga gaacatcagg
     1801 gaagcgaagg ttaaaccaag caggcttttt ttcatttttt atactccata gtcagatgac
     1861 gactttccca gcatgcgctt cagagtgtcg tctttatcga tgaaatgatg cttcagggcc
     1921 ataaatccat gcataacgga cagtactacg acgctccacg cgagccaaaa atgcagggca
     1981 ccggcaaagt cagcctgtgc gccagcgtcg gcgagggtcg cggggacgtc aaaccagcca
     2041 aaaacgctga tcggtttacc atcggcagtt gagatcagat agccgctgat gccgatggcg
     2101 aacagcaata ggtacaatgc gagatgtcca gcctttgcgc caagacgagt cattgacgaa
     2161 taactttgca gcggacccgg cggtggagat atgacgcgcc acagaacgcg aataaccagc
     2221 cccatcatta acaaaatacc gatgctttta tgcagttcgg gtgctttgtg ataccagcca
     2281 tcgtaataac tgagcgtgac catccacaga cctaaagcaa acatgccgta gacaataatt
     2341 gcgcttatcc aatgtaaggc cgcagatata acaccatagc gttcaggggt atttgtgaat
     2401 gacataagtg catccaaaac gaatttgcga gagaaagaaa atggcgtgtc ggagaatgaa
     2461 ttgcaacaca aaaataacaa atcgaatgat atttattttt atttcaataa ttttgaatta
     2521 ataatgaaag aatcttcata agtttagtgg gttcagcatc agagatgtct ggtgagtgct
     2581 gttgaagcag gaataatctc attcaatttt tcacgctaaa tgaaagggag attaaatatt
     2641 tgtcagttta tgtcagtgcg gaaaactaaa aataaaacac atagatcaga tccataattg
     2701 ccaacaatcc ccagagaaaa agataaagca tcagatgctc gcgaatatta ttgatgagat
     2761 agtgcaacag ccgacgcatt aaatactcct gtaaaaacgg cctcattctg gaggccgtaa
     2821 tgatgattat tggaagcggg aaagcctgaa tggcgtcaaa tcaaaatcgc tttttttgtc
     2881 ttgcgcaaaa tcggcagcta tttcccctaa aactgacgca aatttaaaac cgtgcccact
     2941 caggccggta atgagcagtg tattatcgtg ggcgggtaga gtatcgataa taaaatcctc
     3001 gtcaggagaa ttatcatagg tgcaggcagc gccgtacagg cagcaaccga tacccggcaa
     3061 cacattgcgc aagaacggga aggcttccga accatcgctg accacttccg caaacggaac
     3121 acgttcatcc gctgaatgga taacctggcc tccgttatgt ttgccaatct tcaacgcgtc
     3181 gttttctgcc ggaaaaccat aatattgatc gccattgggc agttcaccgg taaacgccgg
     3241 gaatttattc ttcacgctat agcgaccatc ggcctgatac caggcaaata ctttgcgtac
     3301 gggctggaca ggcagctccg ggagtaggtc ttttacccat gttcccgcgc agacaatcgc
     3361 ttttttcgcc tgatactcac cgtctgccgt ttcaatagtt acgccatcat cgtcatgacg
     3421 aatttcggtg accgggcagt tgaacagctg cgcacagccc gcttccttcg ccagttggat
     3481 ccaggtttta atcgccagtt cgctgcgcaa aaaaccggaa tcagtttcaa ataaaccgat
     3541 gtagttgtcc gggacgcgta tttctggcca gcgggccata atcccttgcg cgtcgagctg
     3601 ttcaacgttg agttgccact gttcggcgct gtgggcgacg ttggcgagaa atgctgagtc
     3661 agccggaccg aggttaatga caccagagcg tacaaaaatg ggatcatctt cgttgtggcg
     3721 ggaaagttca tcccacagca tttgcgcgcg gaggaccagc gggacatact tttcgccttc
     3781 accataagca tggcgaatta atcgcgtatc gccgtggtgg ctgccgtgtt gatgcggtgg
     3841 catatgggcg tcggtcatta gcacgtttaa accggcgcgg gttgcataat acccggcggc
     3901 agcgcctacg gaaccgctgc caataatgat gagatcgtat ttcatttttt tctctctgct
     3961 ctcatggttt aagcagagta attaactgaa tgcagatata caacccagaa atggtaaagg
     4021 caccggtgag gtgccttttg ggtggatggt catgtcatgt taatgacgcc gatactcgtt
     4081 taccgggaaa tcaccggatt caattttggc gattcctgct tctaatatcg aaataaattg
     4141 tctggcaaca tcagtggtta accagagcgt ctgaccaact tcagtccctt cctgctcgga
     4201 cttatttggg gtctggtagt gcaaacgcaa catcagcgca tcatagctat caacggtgct
     4261 gatgtcccac cctacgagcg gatgagtctg aatgacttca ttattttttt ccatcatggc
     4321 cccctaattc gtgttactag acaacgttat tcgaggttca atgcgttttt atctgaagca
     4381 acttcagtat accaataagt aagggtattc accgaatttt taaagggggg aacagataaa
     4441 tttctgcttt taagatttat atgaacaata aaacagcatg tcattcatat ttttttagca
     4501 tattgtgcaa ttattttgag gaagtgtaga aattttgtac tcacaattcg caagtaaaat
     4561 aaaaaagccg gggcgacccg gcaaaaacaa aaatcactgc atattattcg ctgacaaacc
     4621 agtcatcggc gctttcccac gtttcctgga gaatttcgct aatgcgctgt ttatcttctt
     4681 ttgttgcgcc aataaccgat aaattattcg ctgcggcata acgtaccgat acgtggcctt
     4741 cattatcagg aaacgcatac tgaatacggc gggaaagttc gccagccagg gcgtcaatag
     4801 ccccagctgg caatggagaa gttttcgcta tggtgacttc aattcgcata atagccccct
     4861 gttgaatata ctggttattt atacaggcaa aataacctaa tgacaacagg aagctacgat
     4921 ttttattgtt taacggacca gcgtaccgtt tccccagcga ggaatggcac cagggtgtca
     4981 tcagtcagtg cgatgctttc agcaacctgt tgctcttcac gtaccagttc gatgaatgtg
     5041 tcgttgaccg gcaagccata gaactgcggg ccgtttacag aacagaatgc ttcaaagtgc
     5101 tgcaaagcat tcatctcttc aaagacggta gcgtaactgc ccagcgcggt tggggcgttg
     5161 aaacagcccg cgcagccgca gctgctctct ttgcgatgac gtgcatgtgg cgcagaatcc
     5221 gttccgagga atacacgatt aaaaccgctg gcgaccagtt cacgcaatgc ctgttggtgg
     5281 atattgcgtt tgaggatggg tagacaatac aggtgcggac gcacgccacc aaccagcata
     5341 tggttgcggt taaacatcag atgctgcgga gtgatggtgg cagccagccg ttcatttccg
     5401 tcacggacat agtcggcagc atctttggtg gtgatgtgct caaaaacgac tttcagcgca
     5461 gtcagacgct ggcgtagcgg ttccatcacg ctttctataa agcgcgcttc acgatcaaaa
     5521 atgtcgatat ctgcatgtgt cacttcacca tgcaccagta gcggcatacc gattttttcc
     5581 atgcgctcaa gtaccggcat gattgcgtca actgacgtaa cgccgtggct ggagttagtg
     5641 gttgcgtttg ccggataaag ttttgcagcg gtgaacacgc cttcgttaaa tccgcgctcc
     5701 agctcattag gatccagcga atctgttaaa taacaggtca tcagcggggt gaaatcgtgc
     5761 ccggcaggta cggcgtcaag aatgcgctgg cgatacgcca cggcagcctc aacggtggtc
     5821 acgggcggag ccagattggg cattacgata gcccgtccat aaatttcgct ggtatacggc
     5881 acgacagttt ttaacatgtc gccatcgcgg aggtgaaggt gccagtcgtc tgggcggcgg
     5941 atctttaata cctgggatgg tgcagtcatt aatctctatg ctccggctga agggatgttt
     6001 ttgccggaca caaaggataa gcggaaacgt tttcctttgc acgaaaaata aagggcgcga
     6061 atgcgccctc gtgattaatc agtaaacgga atgacaattt cgcctggctt cacttcaatg
     6121 cctttcgcca gtttcttcgc cattgcttcg ccctggctgc catcttcgcg caggacgtaa
     6181 gcaggttgct ggttaaagta attgcgtaat gcctggttca aataggggag caacgtttgc
     6241 atcaccgttt gcattttttc cggttgtacc gtcgcatcga ccacttcaat ctctttcagg
     6301 aagatcgcgc ctttttcttt atcaaacacg ggcagcgctt tcagcttcag tttcatggtc
     6361 gctttctggc taccgaacag ggagttcatg tccagattgg cgtctccggt tagggtaacc
     6421 ttattcggct cttcgcgacc aatttggctg gtcaggtttg tcagaacaat atgggcgtca
     6481 gccacgccgg gtaaaccgat atcttttgag aaattattat gtttcgcaag cgactggtta
     6541 atttcttgtt cggtgatggt gtattgggtg agttgattac agccaacgag caggccactg
     6601 acgatcaatg cagcggcaaa taaaaacttg ttcatggtag tcctcgacat gaaatctgcg
     6661 tcaatatcct gacacaacgc agcatgtgtc accagcgata aacgcgccag cagaaaaaac
     6721 tgaaaacggc ggcaacccgc gaatacaggc tgccgcggcg ggtcagtatt aaatcgccat
     6781 tgatgataac aaattgattt gtgtctgttt cgccatatta tcgcggtaat cagcaacgcg
     6841 gcttggccag ttaattccgg ctaccagcgt cagattacgc agcagcggaa atagctgaat
     6901 atcatcttcc gaaagttcgc cattcacggc gttcggtttg acgatcagtt tgtccagcgc
     6961 acgtaaatca tcgctgatat tcttaatcag accgtcagag tgggccagca ggtcggcaaa
     7021 attacccgcg ctggcctctt tcttgtcgac gaaatatttg cgcgcggcag gagtagaaaa
     7081 ctcatcaaat gccgatttgg caaaacgcgg caacagcagt ttgttggcgt agccattgac
     7141 cttgcgcagc cactcttcaa tggcagggga acgtttgccg gtcagtaacg gtttgccgtc
     7201 gagtttatcg acatagtgaa cgatgtccat gctttctggc atatagcggc tgtcatcttt
     7261 ttgcagaatg ggaacctgtt tttgaccgac catccgggtg ggtgtttctg cgtcgtcgtt
     7321 gagcagaaca tgtaattcga cggggatatt tttcaggccg aaaatcatgc gggctttgag
     7381 gcagtaaggg cagtgatcgt aaatgtatag cttcacgtga ctcctccatt tggctgtcag
     7441 ttcactttca gtatggagga gtcagggggg agtatcaaat caggcgtcgc gttcaagcaa
     7501 acgacgcgcg gtgcgtttct ggctaaactg ccaacccagc gcaaggaaag tgaagatgcc
     7561 aataatgccc agcatcatcc acggaagctc tggctggtgc gccgatttgc ccaggtcaaa
     7621 cagccagccg ccaccgatat aaccaatagc gccgccaatc gccagaccca gacggctaaa
     7681 ccccatatag ctgccgcgag ctcttgcgtc tgccagcgaa gcacttaagg tttcacgcgc
     7741 aggctcggca atgatcgacc cgatataaaa cagacaaatc agggtgaaaa gttgttgcag
     7801 accgctgacc atgcccactg gcatcatgct taatgacatt atcaacagcc cagccatcaa
     7861 ccgatgttcc agacgaaaat gcttttcact ccagcgggcg ataggataga gcaacgttaa
     7921 cgacagacac gcttcaatgg catacatcca tttaacggca gagggcgcgc cagccacgtc
     7981 gttgaccata attggcagca tcagcatcac ttgtacagcc agcatgtagt aacccgccag
     8041 cgtcaggaca taggtgacaa aacgcttgtc gcgcatcacg cgggtcatgc cttcgcgaac
     8101 gggcgtgcgt acggtggaga gtttccatgc cggtaacaac cacgcattga acgctgcaca
     8161 cagcacaaac aaaactgccc ctgtggcgca gaccaggcga aagtcgtatt gcaacagcca
     8221 gctccccaac aatgcgccaa tgaccgcacc ggcactgtcc tgcatcatca acagcgaaaa
     8281 aaaacgacca cgctgttgtg gacggattag tttcaccacc agcgccgaac gcggaggatc
     8341 aaacaacgtg ccgccgagtc ccgaaagcag gcatgaaaac cacagtagcc acggttcgtg
     8401 ggcgataccc attgtggcga atccggcggc acgcatcagc ataccggtaa caatcatcgg
     8461 tttggcacca aagcggtcgg caattgctcc gccgaaaata cccagacctt gctgaataaa
     8521 ctggcgtagg ccgagggcaa taccgaccat gacggcggcc cagcccattt gatcaacgaa
     8581 gcggatagag atcagcggga agacaacaaa gaaccccagc acgaccagca tattatcgat
     8641 gagcaggaaa tatttaccca ggttcctcgc ctgcgacacg cgggacattt cccctcccgg
     8701 gaaataaaag atgagcactt tctattctgc attgccgcta agacttttcc catcacttcg
     8761 gggacaatat ttttttatca aaacgtcgct ttgatagaga gtttttatca aatatgtgaa
     8821 taatgcagaa aatggcattc tggacttttc acagggtctg gttgcgcagg tatagtaata
     8881 cttacagcgt attaaagacg ttacgggaag gagtaggtat agaatgtttg gctatcgcag
     8941 taacgtgcca aaagtgcgct taaccacaga ccgactggtc gtgcgtctgg tgcatgatcg
     9001 tgatgcctgg cgtcttgcgg attattacgc agagaatcgc catttcctca agccctggga
     9061 gccagtgcgc gacgaaagcc actgttatcc atcaggctgg caggccaggc tagggatgat
     9121 taacgaattt cataaacaag gttcagcttt ctactttggc ttattcgacc cggacgaaaa
     9181 agagattatt ggcgttgcca atttttccaa tgttgttcgt ggctcttttc atgcctgcta
     9241 tctcggttat tcgattgggc aaaaatggca gggcaaagga ctcatgtttg aagccctgac
     9301 cgcagccatt cgttatatgc agcgcaccca acatattcat cgcattatgg ctaattatat
     9361 gccgcacaat aaacgcagcg gtgatttact ggcgcgactg ggttttgaaa aagaaggcta
     9421 tgcgaaagac tatctgttga ttgatggaca atggcgcgat cacgtactga cggcattaac
     9481 taccccagac tggacgcccg gccgctaagg agaatctcat gaaatatcaa cttactgccc
     9541 ttgaagctcg cgtgattggt tgcttgctgg aaaagcaggt gaccacgccg gagcaatacc
     9601 cgctctcagt caatggtgta gtcacggcct gtaatcagaa aacgaaccgt gaaccggtca
     9661 tgaatctgag tgaatccgaa gtgcaggaac agctggataa tctggtcaaa cgtcattatc
     9721 tacgcacagt gagcggtttt ggtaatcggg tcaccaaata tgagcaacgt ttttgtaatt
     9781 cagaatttgg cgatctgaaa ctgagcgccg cagaagtggc gttaatcacc acgttgttat
     9841 tgcgtggtgc ccagacgccg ggtgaactgc gcagccgcgc cgcgcgaatg tatgaattca
     9901 gcgatatggc ggaagtggag tcgacgctgg aacaactggc aaatcgcgaa gatggtcctt
     9961 ttgtggtgcg tctggcccgc gaaccgggta aacgtgaaag ccgctacatg catcttttca
    10021 gtggtgaggt tgaagatcag ccggcggtga tggatatgtc gaacgcggtt gacggtgatt
    10081 tacaggcacg cgtcgaagcc ctggaaatcg aagtggcaga actgaaacag cgtcttgatt
    10141 cgttgctggc ccatctggga gattaaagtg aaaaaattac gtatcggcgt agtggggtta
    10201 ggcggcatcg cgcaaaaagc gtggttaccg gtgctggcgg cagcgtctga ctggacgtta
    10261 caaggtgcgt ggtcgcctac gcgcgccaaa gccctgccaa tttgtgacag ctggcgcatt
    10321 ccttatgctg attcgttatc cagccttgcc gccagttgcg atgcggtttt tgtgcattcc
    10381 agcaccgcca gccactttga cgtggtcagt acgttactca atgcgggggg acatgtctgt
    10441 gtcgataaac cgctggcaga aaatctgcgc gatgctgaac ggctggtgga actggcggcg
    10501 cggaaaaaat tgacgttgat ggtcggtttt aaccgtcgtt tcgcaccact ctacggtgag
    10561 ttaaaaacgc aactcgccac cgcagcctcg ctaagaatgg ataaacatcg tagcaatagc
    10621 gtcgggccac acgatcttta tttcacgttg ctggatgatt atctgcatgt ggtggatacc
    10681 gcgctgtggt tgtcgggcgg taaagcctct ctggatggcg gtacgctact gactaatgac
    10741 gctggcgaaa tgctgtttgc cgagcaccat ttttcggccg gtcctttgca gatcaccacc
    10801 tgtatgcatc gccgtgccgg aagtcagcgt gaaaccgtgc aggccgtgac tgacggtgcg
    10861 ctcatcgaca ttacggatat gcgcgaatgg cgtgaggagc gcgggcaggg cgtagtgcat
    10921 aaaccgattc ttggttggca gagtacgctt gagcaacgtg gatttgtcgg ctgtgcgcgg
    10981 cacttcattg aatgcgtgca aaatcagaca gttccgcaaa ccgccggcga acaggcagtg
    11041 ctggcgcaac gtatcgttga caagatctgg cgcgatgcga tgagtgaata aaccctgtaa
    11101 catctggcgg tagcaattca ccataatcca ggtagactat tcgcctcttt cagcgcctgc
    11161 cttgcaggcg ttttgcccgt gggtctggat tagaacaccg atgaatttat taaaatcgct
    11221 ggccgccgtc agctcgatga ccatgttttc gcgtgtgctt ggcttcgcac gtgacgcaat
    11281 tgtcgccaga atctttggcg cagggatggc aaccgacgcc tttttcgtcg cttttaaact
    11341 tcctaacttg ttgcgccgta tttttgccga aggggcattt tcccaggcat ttgtaccgat
    11401 tctggcggaa tataaaagca agcagggtga agacgccacg cgggtctttg tctcttatgt
    11461 ttctggcctg ctgacacttg cgctggcggt tgtgacggtc gctggcatgc tcgctgcacc
    11521 gtgggtgatc atggtgaccg cgcctggctt cgctgacact gccgacaaat ttgccctgac
    11581 cagccagcta ctaaagataa cctttcccta tatcttgctg atctccctgg cgtcgctggt
    11641 gggagcgatt ctgaatacct ggaaccgctt ctcaattccg gcgtttgccc cgacattgct
    11701 caatatcagc atgattggct ttgcgctgtt tgccgcaccg tactttaatc caccggtgct
    11761 ggcgctggcc tgggctgtta cggtcggcgg catcctgcag ctggtgtatc agctaccgca
    11821 tctgaagaag atcggcatgc tggttttgcc acgcattaac ttccacgatg ccggagcaat
    11881 gcgcgtggtg aaacagatgg gaccggcgat ccttggcgtc tctgtgagcc agatctcctt
    11941 aatcatcaac accatttttg cctcgtttct tgcttccggt tcggtgtctt ggatgtatta
    12001 cgccgaccgc ttaatggagt ttccgtccgg tgtactgggc gtggcgcttg gcaccatttt
    12061 gctgccgtcg ctgtcgaaaa gttttgccag tggcaatcat gatgaataca accgtttgat
    12121 ggactggggg ctgcgtcttt gtttcctgtt ggcgctgccg agtgcggttg cgttgggcat
    12181 tctttccggt ccgttaaccg tttcgctgtt ccagtacggt aaatttaccg cctttgatgc
    12241 gctgatgacc cagcgggcgt taattgccta ctcggtgggt ttgattggcc tgattgtagt
    12301 gaaagtgttg gctcctggct tttattcccg ccaggacatt aaaacgccag tgaaaattgc
    12361 catcgttacg ctgattttaa cgcaattgat gaacctggcg tttatcggtc cgttgaaaca
    12421 tgccgggctg tcactttcta ttggtctggc ggcgtgtctg aatgcttcgc tgctttactg
    12481 gcagttgcgt aagcagaaaa tctttacccc gcaacctggc tggatggcgt ttctgttgcg
    12541 tctggtagtg gcggtactgg tgatgtcggg cgtgctttta ggtatgttac atatcatgcc
    12601 ggagtggtca ttgggtacca tgccctggcg cttactgcgt ttaatggcgg tcgtgctggc
    12661 ggggattgcc gcgtacttcg ctgcactggc ggtactgggc ttcaaagtta aagaatttgc
    12721 ccgccggacg gtgtaacaat gcattccggc ctgcagtgca ggccggagat aatcttcaga
    12781 tcgaaatctt tttactgccg cgatgggttg ttgaggtctg accgttcgcc ccatatagcg
    12841 tcggttcctg atgcggtttc aacatttcca gcgcctgttg attgcgctca atctgtcctt
    12901 ccagtaacca gccgttatgt tgattcattt gacgtagttg ctgcgttttc acagtaattt
    12961 cctgccagcg ttgactaata tcaacgctat ttgctgaatt gggttctttc ctgcgtaact
    13021 gttcgaggta atccagcgtc gccagcagtg agcttttttg ttctgtaatc cattgcaact
    13081 ggctgccgtt gatctgcccc attgagagat gttgctgctc ttgatccatt accgttttga
    13141 gatcgttaag cacagcggac atctggtcga ggatctctgc aagacgcgtc atacgatcag
    13201 ttactctgca agtcttgctg cgcttcgttg atcagcgcat cggcaatttt gccggtgtcc
    13261 atttttagtt caccgttacg aatcgccagc tttaacgctt cgacacgttc aggattgata
    13321 tcactgctgc cgggttgcat cagttttgct tgcgcgtcgc ttaacgtcac actggtgctg
    13381 gtggcggcgg ttgtttttgc cgcccgggta ttcgttaccg gcgcgtcagt ggtttcacgc
    13441 ggttgaacgg tgcttacggg cttcagaggt gaagtgcggt caatactcat ggtttattcc
    13501 tcattgaggg cgcttttatc atgtgttgct tatttatcgg caagggacgg gtaatcttta
    13561 acagcttaca ggtttataag aatattccca tctgcatcaa caacgccgct gaccacctgt
    13621 cccgatacca tgcgcacccg cgcattctgt gcgacggcag cattgttcag cgcctgacct
    13681 tctgcgttgg cgctaaaccc atcaccgctg gcgatcacgt tgacgcgttg tcccgctttt
    13741 acccgccatg cctggcgaaa ctgggttaac tggataggtt gatcgggtga tatatcgcgc
    13801 aggctaacgg catcaacaag ttgattgata tccagcaccg tacgcggtgg cagggtatcc
    13861 agcctgccgc gtttcagttt gacattgcca gcttccagtt ttcctccccg cacaatgggc
    13921 atcgcggcaa ccacataatt tcctgtagcc tgcacattaa cttgtaaata tcgtttgtcg
    13981 ttaccgcagc gagccaacac attcacattg ccccacaggc gggaattatt gctcatcgaa
    14041 agcaatggct gctcgcacgg cggtagtaga ttgggcgctg tacgaataga aacacgaacc
    14101 tcatcactta cccccgcgag ctgggcgcta aaaaaggtgt gcaattgcga tgtgagattg
    14161 ctcgccgcac ttaacggact gaacagtatc gcgatgatcg ccacgcgacg ttttattgcc
    14221 agcattttcg ccccccagcc atttctacaa cgtgaattgt acctgtccgc aatgaccatc
    14281 aacggcataa atagcgaccc attttgcgtt tattccgccg ataacgcgcg cgtaaaggca
    14341 tttaagctga tggcagaatt ttgatacctg cggaggagat atgctcgata agctcgacgc
    14401 cgccttacgt tttcaacaag aggcgctcaa tctgcgcgcc cagcgtcagg aagtgctggc
    14461 agcaaacatc gccaatgccg atacccctgg ttatcaggct cgcgatatcg attttgccag
    14521 tgaacttaaa aaagtcatgc aacgtggacg ggatgcaaca agtgtggttg cactgacgat
    14581 gacctcaacg caacacattc cggcgcaggc gctgacgcct cctaccgcag aactgcaata
    14641 ccgtattccg gaccagcctt cgcttgacgg taataccgtc gatatggatc gcgaacgcac
    14701 ccagtttgcc gataacagcc tgcaatacca gatgagcctt agcgcgttga gcgggcaaat
    14761 caaaggcatg atgaacgttt tacagagcgg aaattaacgg atggcactgc tgaatatttt
    14821 tgatatcgcc ggttcggcgt taactgccca gtcccagcgc ctgaacgtgg cggccagtaa
    14881 tctggcgaat gctgatagct tgaccggtcc cgatggacag ccatatcggg caaaacaggt
    14941 ggtatttcag gttaacgctg cgccaggtgc tgcgacaggc ggcgtaaaag ttgccgatgt
    15001 tatagaaagt caggccccgg acaaactggt ttatgaaccg ggtaatccgc tggcggatgc
    15061 aaagggctac gtgaaaatgc caaacgttga tgtcgtcggg gagatggtta acaccatgtc
    15121 ggcgtcacgc agttatcagg ccaatgttga agtgctcaac acggtgaaaa gcatgatgct
    15181 gaaaaccctt acgctcggtc aataaaggag aaagctatgt ccattgcggt aacaaccacc
    15241 gatccgacaa ataccggcgt cagcaccacc agtagtagtt cgctcacggg cagcaacgcc
    15301 gcagatttac aaagcagttt tctgactttg ttggtggcgc agctgaaaaa ccaggacccg
    15361 accaatccaa tggaaaacaa cgagctgacg tcgcaattgg cacaaatcag cacggtcagc
    15421 gggattgaaa aactcaatac cacgctcgga tctatttccg gacagattga taacagccag
    15481 tcgttacagg ccagtaacct gatcggtcac ggtgtgatga tccccggtac cacagttctt
    15541 gcgggaaccg gcagtgatga aggggctgtg accacgacca cgccgtttgg tgttgagctg
    15601 caacaggcgg cagacaaagt tacggccacc atcaccgata aaaacggtgc agttgtccgt
    15661 accattgata tcggtgaact gactgccgga gtccacagtt tcacctggga cggtacgttg
    15721 actgacggca gcactgcgcc gaacggttct tacaatgtag cgattagcgc cagtagcggt
    15781 ggtacacaac tggttgccca gccgctgcag tttgctctgg tgcagggtgt gatccgcggc
    15841 aacaacggta atacgctgga tctcggcact tacggcacca ccaccctcga cgaagtacgg
    15901 cagataattt aagccttcac atttcaggag tcagtcatgg ccttttctca agcggttagc
    15961 ggattaaacg ctgccgccac caacctcgat gttattggca acaatatcgc caactccgcc
    16021 acctacggct ttaaatcagg cacggcctct tttgccgata tgtttgccgg ttcgaaagtg
    16081 ggactggggg taaaagttgc cggtatcact caggacttta ccgatggcac gaccaccaac
    16141 accgggcgag gtctggacgt tgctatcagc cagaacggtt ttttccgtct ggtagacagc
    16201 aacggttcgg tgttctacag ccgtaacgga caatttaagc tggatgaaaa ccgtaacctg
    16261 gtgaatatgc aaggtttaca gctgacgggt tacccggcaa ccggtacgcc gccgactatt
    16321 cagcaagggg cgaatccgac caatatttcg atcccgaata ccctgatggc agcgaaaact
    16381 accaccacgg cgtcgatgca gatcaacctg aattccagtg atccgcttcc ctctgttaaa
    16441 gcattcgatg ccagcaatgc ggatagctat aacaaaaaag gttcggtgac tgttttcgat
    16501 agtcagggta atgctcatga tatgagcgtt tattttgtga agaccgggga taacaactgg
    16561 gatgtttaca ccctggatag cagtgatcca acaggcacag ctaaccctgc aacgacgctg
    16621 gtgtttaatg ccaatggcgt tctgacctca gatccaacaa aggatattac caccggcgca
    16681 attaacggcg cagatcccgc cacgtttagc ctgagcttcc tcaactccat gcagcaaaat
    16741 accggcgcga acaacattgt ggcaaccacc cagaacggct acaaaccggg cgatctggtg
    16801 agttatcaaa tcaatgatga cggtacggtt gtcggcaact attccaacga acaaacccaa
    16861 ctgctggggc agattgtact ggcgaacttt gccaacaacg aaggtctggc atccgaaggc
    16921 gacaacgtct ggtctgcgac gcaatcttct ggcgtggcgc tgttggggac agccgggacg
    16981 ggcaactttg gcaccctgac caacggtgcg ctggaagcgt ccaacgtcga tctcagtaaa
    17041 gaactggtca atatgatcgt tgcccagcgt aactatcagt ctaacgccca gaccatcaaa
    17101 acccaggacc agatcctcaa cacgctggtt aacttacgct aatcgctgac gggatagctc
    17161 aatggatcac gcaatttata ccgcgatggg agcagccagc cagacgctga atcaacaggc
    17221 ggtaaccgcc agtaatctgg ccaatgcctc tacgcccggt tttcgcgcgc agttaaacgc
    17281 cttgcgcgca gtgccggtgg aagggctttc actgcccacg cgcacgctgg taacggcgtc
    17341 aacgccgggt gcagacatga cgcccggtaa gatggattac acctcgcgcc cgctggacgt
    17401 tgcgttgcag caggatggct ggctggccgt gcagaccgct gacggtagcg aagggtatac
    17461 gcgtaatggc agcattcagg ttgatcccac cgggcaactg acaattcagg gacatccggt
    17521 gataggcgag gctgggccaa ttgctgtgcc ggaaggggcg gaaatcacta ttgctgccga
    17581 tggcacaatc tcggcgctga atccgggcga tccggcaaat acggttgcgc cagtggggcg
    17641 tctgaaactg gtgaaagcca cgggcagcga agtgcagcgc ggtgacgacg gcatttttcg
    17701 tttaagcgcg gaaagccagg ccacgcgtgg gccggtactg caggcagatc caaccttgcg
    17761 tgtgatgtcg ggagttctgg aaggcagtaa cgtcaatgcc gttgcggcaa tgagcgacat
    17821 gattgccagc gcgcggcgtt ttgaaatgca gatgaaggtg atcagcagcg tcgatgataa
    17881 cgcaggccgt gccaaccaac tgctgtcgat gagttaatta aacgattgat gacattgcaa
    17941 taaaagcgcg cggactattc tctcgcccat tttgggagag gttagggtga ggggaaattc
    18001 gcaccgtcgc gtgcttctgc tctgcttgtc actaatccac tacaggacaa tttatgatca
    18061 gttcattatg gatcgccaaa acgggccttg acgcccagca aaccaatatg gacgtcattg
    18121 ccaacaacct ggcaaacgtt agtaccaacg gttttaagcg tcagcgcgcg gtgtttgaag
    18181 atctgctgta tcaaaccatt cgccagccgg gggcacagtc ttccgaacaa accaccttac
    18241 catccggatt acaaatcggt acgggggtac gcccggtcgc cactgaacgc ttacacagcc
    18301 agggcaacct gtcgcaaacc aataacagca aagatgtcgc gattaaaggg cagggctttt
    18361 tccaggtgat gttgccagat ggctcatcag cctatacccg tgacggctct ttccaggtgg
    18421 atcagaacgg gcagctggtg acggctggtg gttttcaggt gcagccagcg atcaccattc
    18481 cggcgaatgc gttaagtatc accatcggtc gtgatggcgt ggtcagcgta acccaacaag
    18541 gccaggcagc tccggttcag gttgggcagc tcaatctcac cacctttatg aatgataccg
    18601 gactggagag tattggcgaa aacctctaca ccgaaacgca atcctccggt gcaccgaacg
    18661 aaagcacgcc gggcctgaac ggcgcgggac tgctgtatca agggtatgtt gaaacgtcta
    18721 acgtcaacgt ggcggaagaa ctggtcaata tgattcaggt gcaacgcgct tacgaaatca
    18781 acagtaaagc ggtatccacc atcgatcaga tgctgcaaaa actgacgcaa ctctaaggca
    18841 taaccggtgg cagattcacc ggtttactga tttttgaaga tgatagccat gcaaaaaaac
    18901 gctgcgcata cttatgccat ttccagcttg ttggtgcttt cactaaccgg ctgcgcctgg
    18961 ataccctcca cgccgctggt acagggggcg accagtgcac aaccggttcc cggtccgacg
    19021 cccgtcgcca acggttctat tttccagtct gctcagccga ttaactatgg ctatcaaccg
    19081 ctgtttgaag atcgtcgacc acgcaatatt ggcgatacgc tgaccatcgt gttgcaggag
    19141 aacgtcagcg ccagcaaaag ctcctctgcg aatgccagcc gtgacggtaa atctaatttt
    19201 ggctttgata ctgtgccgcg ctatttgcag gggctgtttg gtaacgctcg tgccgatgtc
    19261 gaagcctccg gtggtaacac gttcaacgga aagggcgggg ccaatgccag caataccttt
    19321 agcggcacgt tgacggtgac ggttgaccag gtactggtca acggcaacct acatgtggtg
    19381 ggtgaaaaac agattgccat taatcagggt accgaattta ttcgcttctc tggcgtggtt
    19441 aatccacgca ccatcagcgg cagcaatacc gtaccgtcta ctcaggtggc ggatgcgcgc
    19501 attgaatacg taggcaatgg ctacattaac gaagcgcaaa atatgggctg gttgcagcgt
    19561 ttcttcctta acctgtcgcc aatgtaagtg aggctgtggt gattaaattt ctctctgcat
    19621 taattcttct actggttacg acggcggttc aggctgagcg tattcgcgat ctcaccagtg
    19681 ttcagggggt aaggcaaaac tcactgattg gctatggcct ggtggtgggg ctggatggca
    19741 ccggtgacca gacaactcag acgccgttta ccacacaaac gcttaataac atgctctcac
    19801 agctgggaat taccgttccg acgggcacca atatgcagct aaaaaacgtc gctgcggtaa
    19861 tggtgacggc gtcacttcca ccgtttggac gccaggggca aaccattgac gtggtggttt
    19921 cttccatggg aaatgccaaa agtctgcgtg gcggtacgtt attgatgaca ccgcttaagg
    19981 gcgttgacag tcaggtgtat gcgctggcac agggcaatat tctggttggc ggcgcagggg
    20041 cctccgctgg cggtagcagt gttcaggtga accaactgaa cggtggacgg atcaccaatg
    20101 gtgcggttat tgaacgtgaa ttgcccagcc agtttggcgt cgggaatacc cttaatttgc
    20161 aacttaacga cgaagatttc agtatggcgc agcaaatcgc tgacaccatc aaccgcgtgc
    20221 gtggatatgg cagcgccacc gcgttagatg cgcggactat tcaggtgcgc gtaccgagtg
    20281 gcaacagttc ccaggtccgt ttccttgccg atatccagaa tatgcaggtt aatgttaccc
    20341 cgcaggacgc taaagtagtg attaactcgc gcactggttc ggtggtgatg aatcgcgaag
    20401 tgaccctcga cagctgcgcg gtagcgcagg ggaatctctc ggttacggta aatcggcagg
    20461 ccaatgtcag ccagccagat acaccgtttg gtggcgggca gactgtggtt actccacaaa
    20521 cgcagatcga tttacgccag agcggcggtt cgctgcaaag cgtacgttcc agcgccagcc
    20581 tcaataacgt ggtgcgtgcg ctcaatgcgc tgggcgctac gccgatggat ctgatgtcca
    20641 tactgcaatc aatgcaaagt gcgggatgtc tgcgggcaaa actggaaatc atctgatgat
    20701 cagcgacagc aaactactgg caagtgcggc ctgggatgca caatcgctca acgaacttaa
    20761 ggcgaaagcg ggcgaagatc cggcggcaaa tatccgtccg gtggcccgcc aggtggaagg
    20821 gatgttcgtg cagatgatgt tgaaaagcat gcgcgacgct ttaccaaaag atggcctgtt
    20881 cagcagcgag cacactcgcc tgtataccag tatgtatgac cagcagattg cccaacagat
    20941 gacggcgggc aaaggtctgg ggctggcaga gatgatggtt aaacagatga cgccagaaca
    21001 accattgcca gaggagtcca tgccagcagc accgatgaaa ttcccgctcg aaaccgtggt
    21061 gcgttatcaa aatcagacgc tttcgcagct ggtgcaaaag gccgtaccac gtaactacga
    21121 tgattcgctg ccgggtgaca gtaaagcatt cctcgcgcaa ctctcgttgc ccgcccaact
    21181 ggcaagccag caaagcggtg tgccacatca tttgatcctc gctcaggcgg cgctggaatc
    21241 tggctgggga caacggcaaa tccgccgtga aaacggcgag ccgagctata acctgtttgg
    21301 cgtcaaagcc tctggcaact ggaaagggcc agtcactgaa atcaccacga ctgaatatga
    21361 aaatggcgaa gcgaagaaag taaaagcgaa gtttcgggtc tacagctcgt atctggaagc
    21421 attgtcggat tacgttgggc tgttaacgcg taacccgcgc tacgccgccg tgacgaccgc
    21481 cgcgagtgcg gagcaggggg cgcaggccct acaggacgcg ggctatgcca ccgatcctca
    21541 ctatgcccgc aaactcacca gcatgattca gcagatgaaa tcgataagcg acaaggtgag
    21601 caaaacctac agcatgaaca ttgataatct gttctgaata actcaagtcc ggcgggtcgc
    21661 tgccgataat actctgtaat tgaaggctta taaggaacct ccatgtccag cttgatcaat
    21721 aacgccatga gcggactgaa cgcggcccag gcggcgttaa atacggcaag taataatatc
    21781 tccagctata acgttgccgg atatacccgc caaaccacta ttatggcgca ggccaatagc
    21841 acgttgggcg ctggcggctg ggttggcaat ggcgtctacg tttctggtgt gcagcgtgag
    21901 tatgatgcgt ttattaccaa ccagttacgt gctgcgcaga cgcaaagtag cggcctgact
    21961 gcccgctatg agcagatgtc gaaaatcgac aatatgctct ccaccagtac ctcttcgttg
    22021 gcaacacaga tgcaggattt cttcaccagc ctgcaaacgc tggtgagtaa cgcggaagac
    22081 ccggcagcgc gccaggcgct gattgggaaa tcagaagggt tggtgaatca gtttaaaacc
    22141 accgatcaat atctgcgcga ccaggacaaa caggtcaata tcgcgatagg tgccagcgtt
    22201 gatcagatca acaattacgc taaacaaatc gccagcctga acgatcagat ctcccgcctg
    22261 acaggcgtgg gggcaggggc gtcacctaac aatctgctgg atcaacgcga tcaactggtg
    22321 agcgaattaa accagattgt tggcgtagaa gtcagcgttc aggatggtgg cacttataac
    22381 atcacgatgg ccaatggcta ctcactggtt cagggaagta cggcgcggca gctggcggca
    22441 gttccttcca gcgccgaccc ttctcgtacg actgtcgctt atattgatgg gacggctggc
    22501 aatattgaga tcccggagaa attgctgaat accgggtcgc tgggcggcat tctgacattc
    22561 cgttctcagg atctggacca gacgcgtaat acgctcggac aactggcgct ggcatttgcc
    22621 gaggctttca acacccaaca caaagccgga tttgatgcta acggcgatgc cggtgaagat
    22681 ttctttgcta tcggtaagcc cgcggttctg caaaacacga aaaacaaagg tgacgttgcg
    22741 atcggtgcaa cggtaactga tgcctccgcg gtactggcga cagattacaa aatctcgttc
    22801 gataataatc agtggcaggc cacccgcctt gccagcaata ccacttttac ggtgacgccg
    22861 gatgccaacg gtaaagtggc atttgatggt ctggagttga cgtttacagg aacgcctgcc
    22921 gttaacgaca gcttcacgtt gaaaccggta agtgacgcca tcgtcaacat ggatgtatta
    22981 atcactgacg aagcgaaaat cgccatggcg agcgaagaag atgtgggtga tagcgacaac
    23041 cgcaacggtc aggccctgct ggatctgcaa agcaacagta aaacggtggg cggtgcgaaa
    23101 tcctttaacg acgcttatgc ctcgttagtg agtgatatcg gtaataaaac cgcgacgttg
    23161 aaaaccagta gcgccacgca aggtaatgtg gtgacgcagc tttccaatca gcagcagtcg
    23221 atttccggtg tcaatctcga tgaggagtac ggaaatctgc aacgttttca gcagtattac
    23281 ctggcgaatg cgcaggttct gcagacggca aacgcgattt ttgatgcgct gattaacatt
    23341 cgctaagggg agataaaatg cgtttcagta cccagatgat gtaccagcaa aacatgcgcg
    23401 gcatcaccaa ttctcaggca gaatggatga agtacggtga acagatgtcg acgggtaagc
    23461 gagtcgttaa cccttcagat gatcccattg ctgcatcaca agctgttgtt ctctcccagg
    23521 cacaggcgca aaacagccag tacacgctgg cgcgtacttt cgccactcaa aaagtgtcac
    23581 tggaagagag tgtacttagc caggtcacca ctgctatcca gaatgctcag gaaaaaattg
    23641 tctacgccag caatggcacc ttgagtgacg atgaccgggc ctcgctggct acggatattc
    23701 aggggcttcg tgaccagttg ctgaatctgg cgaacaccac tgacggtaac gggcgctaca
    23761 tttttgccgg ttataaaaca gaatccgcac cgtttagcga ggtggatggc aaatacgaag
    23821 gcggagcaga aagtattaaa caacaggtcg atgcttcgcg ttcgatggtg atagggcaca
    23881 cgggggacaa aattttcgac agtattacca gcaacgcggt agcggaacca gacggtagcg
    23941 cttctgaaac caatcttttt gccatgctgg atagcgccat cgcagccctg aaaacgccgg
    24001 tcgcggatag cgaagcggat aaagaaaccg ccgctgcggc actggataaa accaaccgcg
    24061 gactgaaaaa ctcgctgaac aatgtgctga ctgttcgcgc ggaattaggc acgcagctga
    24121 acgaactgga gtcgctggat tcattaggta gcgatcgcgc tttagggcaa acgcagcaga
    24181 tgagcgatct ggttgatgtg gactggaatg caactatttc atcttacatc atgcagcaaa
    24241 cggcattgca ggcgtcgtat aaagcattta ccgatatgca gggattgtcg ctcttccagc
    24301 tcaacaaata atttcgcttt aaaacatatc atgaaactgg gtatgttttg tctgcctgct
    24361 ctgggatcgc cggggcgggc atttttttgc atgagacata acgaactatt taagacattc
    24421 ctgcaaatca gccaatgttg ccatcagtat catagaatcg acaggcgatg gttcaaaccc
    24481 cactcgcaga taaaactcac gggcctggtc tgataacgca tggaccaaca ttccgcgaat
    24541 accaatgact tcagccgcct gtagtactcg tagaccggca tctctgacca tcgcacgacc
    24601 gatgccctga ccctgtaatg atttatctat tgccagacga ccaagtacta caacagggat
    24661 cggttctggc atatttcggc gaaatcgtcc tgtcgcgaca tacgtttcaa cggcgctgga
    24721 agcaagcgaa taatatgcca aaacgttgct atacgtatca cagctaacaa atgtacgtga
    24781 tgcgccggca agctgatttt ttagggctct ctgctttagc cagttatcca tcgtttctac
    24841 tcccgagcaa aaagaggtga attgatgcct tgccatgagt ggctcggggg cctgaatcat
    24901 tttttttgct cccacggcgc gggtgtttgc atggttttgc gtaatgcagc attgggcgcg
    24961 gggggctgat ccagacgatt caaaaatagc tggtaatcat cagaatcgat cctgaaaatt
    25021 ctctgctcaa tgagcgcctc ttcagctgcg gctctggcgg cctcaaggac aaattcggtg
    25081 cgattttttc ctcgggcttt agcggcccgg tcaataagat cgcgttctgc gggttttatg
    25141 cgcaagttta aagattcgcg cctggctgga atgttattgg ctgctggcat aaatatatcc
    25201 ttcggctttg cgtggatgat aattatacag cacatgtaac gctaatgtca ttacagcaaa
    25261 gatgctaccc ctgaaataaa aaagccctgg cagttaccag ggcttgatta cttgagctaa
    25321 ttattactca acaggttgcg gacgcgcagg agcggcagag gcatgatgcg ttgccgtatg
    25381 acctcctgcg gcacctttgc cttcgaaggc aaaagtaggg cgctgccagt cactgtgacg
    25441 cggtgcctcc ggaacatatt ccggtgccgg agcgcgcgtc attggcgcgg tagcgtggtt
    25501 atgctcaacg gtgatctctg gttcaaccgc ggctaccgtt tcaacttctg ctgcgactgg
    25561 tgcggcaggt tgagcaacaa cttcaggttc agcaaccaca acctcagcag tttcggcaac
    25621 ttcttcaata tcagccgtct cttcctgcgg ttcaactacc ggttctgcgt gttctgcaac
    25681 ttcctgggct acggcaacat cagactcggt aatcacctgc ggctgttcag ttaccgcggc
    25741 ggcaattact tcaggatgcg tcgtttcaac cacttccggt tgcggttcgg caacgtgaac
    25801 gggggcttct acgacttcag ccacttcttc aacaaccggt gcgctaacaa ccggttcgac
    25861 agcggcggcg acagggacct cagtcaccat cggctgcact tgtacttctt cctgttcgcg
    25921 ctgctcttca acctgtacat cttgtggacg tacaatcgga tagcggatcc agactttgcc
    25981 agaggccagt tccggagacg cgcacgctac ggtcaacggc attggcgatt gggttggata
    26041 acgctcgtca cgatagcgac gacgacgctg accactcacg cgcaggtgac gaggcgagcg
    26101 gcgagaacga cgcggcatgc caccgttgtc acggttatcg gcattgttct cttcttgctg
    26161 ttctggtgca gcttgcgcta cgactggcag cgggactttc accagttctg tgcgtggagc
    26221 tggcgcttcc tgaacaattg gttcgccagc gacagtttct tcaaccaccg gtgcgactac
    26281 cgcttcttcg gctacgcttt gctcgtaacg cactttctga ttgagctgac gctgtttacg
    26341 acgcggctga accggacgta cacgttcttc ctgttcggtt tcctgaacag attgctcttc
    26401 aacattcagc gccttcgctt cttgttgcgc ctgacgttta tcatcgttac ggcggcggct
    26461 acgttcacga cgcggcgctt gctgctcgtc ggtggtacgc gctttttccg ttacctcagc
    26521 ctgctgacgg ctctcacgcg tctcgacagt ctgctgctgt gcctggcgac gattacgacg
    26581 gttttcttcg cgattatcgc tgccttctgt acgttcacta cgggagtcgc ggcgctcatt
    26641 acggtcacgg cggttgttct gacgaggctt gcgacgatcc tgttgacgtt ccggtttcgc
    26701 ttctgctttc ggtgctggtt gctcggccgg tttggtttct tcaccaccgc tgaacagcgc
    26761 tttcagtgcg ccgaagaagc ggctcaacaa ccctggttgt gcaggagatg ctggtgttgc
    26821 cgttgcagct ttaggtgctg gggctacaac aggcgcggca ggttcagctg gggttggcgc
    26881 tggcggcaca tccggcatgg caaaggttgc cagcgcaggt tgttccggac gcttacgttc
    26941 agcgaactct tcttcagacg gcagcgccat cgcttcttca tgcagcttcg gcagcatgta
    27001 gcttaaggtt ggagtttctt cccctttacg cacgcgcagc acatggtagt gcggggtttc
    27061 catctgatcg tttggcacaa tcacacagcg cacgccgtcc tgacgagttt caatcgcatt
    27121 cactgcagaa cgtttttcat tcagcaggta agacgcgatt ggcacaggaa caatggcgtg
    27181 aacttcctga gtgttctctt tcagcgcttc ttcttcgatc agacgcagaa tagagagcga
    27241 caacgattcg ttgtcacgca cggtaccagt accggagcag cgcgggcaga cgtgatggct
    27301 ggattcaccc agtgatgggc tcagacgctg acgggacatt tccagcaggc caaagcgaga
    27361 aatatggctg atttgaatac gcgcacggtc ctgacgcacc gcttcacgca gacgattttc
    27421 taccgcacgc tggtggcgta caggcgtcat gtcgatgaag tcgataacaa tcaggccgcc
    27481 gaggtcacgc aggcgcagct gacgagcaat ctcatcggca gcttcgaggt tagtgttaaa
    27541 cgcggtttct tcgatatcgc cgccgcgggt cgcgcgtgcg gagttgatgt cgatggccgt
    27601 taacgcttcg gtgctgtcga taacaatcga accaccggat ggcagacgaa cttcacgctg
    27661 gaaggcggac tcgatctgcg actcgatctg gtagtggctg aacagcggga tctcgccggt
    27721 gtacagtttg attttgctgc tgaaatccgg gcggcctaat gcagcgatat gctgacgtgc
    27781 cagttcgagc actttcgggt tatcgataag gatttcaccg atgtcctggc gtaagtaatc
    27841 gcggaatgcg cgaacgatta cgttgctctc ctgatgaatc aggaacggag ccgggcggct
    27901 ttcagcggct tttttgatgg cttcccagtg tttcagacgg aagcttaaat cccattgcag
    27961 cgcctcagca gatttgccga cgccagcggt gcgcacgata agccccatgc cttccggcag
    28021 ttcaaggctt gccagtgctt cttttaattc ggtacggtcg tcgccttcga tacgacgaga
    28081 aatgccaccc gcgcgcgggt tgttcggcat cagaaccaga tagctacccg ccagactgat
    28141 aaaggtggtt aatgccgcgc ctttgttgcc gcgctcttct ttatcgatct gaacaatgac
    28201 ttcctgacct tcacgcaaca catctttaat gttgggacga ccatgagcac tgtagttagc
    28261 agggaaatat tcgcgggcaa tttcttttag tgggaggaaa ccgtgacgtt cagcgccgta
    28321 atcaacaaaa gcagcttcca gactcggttc aatgcgggtg attttacctt tgtagatgtt
    28381 tgcctttttc tgctcgtgcc ctggactttc gatatccagg tcatacagac gctgcccatc
    28441 tacaagggca acgcgcaact cttcctgctg agttgcgttg attaacattc ttttcatcgt
    28501 aacttactca ttattcttac attgacgact aagctgcggg caaagtaacg cctttccggg
    28561 tgtgaaccga tggcctcgtg tctagtcgcg tcgccaacct cacggttatc gtcagctcaa
    28621 agaggcgcag agtgtcggtt gcccgttttt catgcggaaa aacagcgcaa ttatcaaaga
    28681 aacagactgg gtattactct ccagaaatat ttccatctac cggtaaggac tgcaacccgc
    28741 agcccgctaa ctgcctgaaa gatcaatacg tcttacgcca ttgctgcgtg gatgatcggt
    28801 cgggcaaaat gggttattcc gtaaaatttc ttgttttaac aaggatggac acggaaacgg
    28861 ccccattatt ccactgcaag ccttgttata gcaagatgac ttttaccatt tatcacccgc
    28921 ttactcacag ttttttcact tcttgctggt gattggttta ataaccatca ataaagtgat
    28981 cacgtagtga agtaataagg tgcaaaacta aatataagca tagaaaaatg agtggcgcga
    29041 atcctgttgg ctaattagaa tcgcccacca tgaaaacaga gactccatcc gtaaaaattg
    29101 ttgctatcac cgctgacgaa gcggggcaac gtatcgataa ctttttgcgt acccaattga
    29161 aaggtgtacc aaaaagtatg atttaccgta ttttgcgtaa aggcgaagtg cgggtgaaca
    29221 aaaaacgtat taagcctgaa tataaactcg aagcgggtga tgaggtacgt atcccaccgg
    29281 ttcgcgttgc tgaacgggaa gaagaggcgg tttcgccaca tctgcaaaag gtggcagcgc
    29341 tggcggacgt catcttatat gaagatgacc atatcctggt gctgaataaa ccttccggta
    29401 cggcggtaca tggcggcagt ggtttaagct tcggcgttat cgaaggtttg cgggcgttgc
    29461 gcccggaagc gcggttcctt gaactggttc atcgtctcga ccgggacact tcaggtgtgt
    29521 tgctggtagc gaaaaaacgc tcggcgttgc gttctctgca tgagcaatta cgtgaaaaag
    29581 ggatgcaaaa agattacctg gcgctggtgc gcggtcagtg gcagtcgcat gtgaagagcg
    29641 ttcaagcgcc gttattgaaa aatattttgc aaagcggcga acgtatcgtg cgtgtcagtc
    29701 aggaaggcaa accgtcggaa acacgcttta aagtggaaga acgctatgca tttgccaccc
    29761 tggtgcgttg tagcccggta acagggcgta ctcatcagat ccgtgtgcat acacaatatg
    29821 cgggtcatcc gattgccttt gacgatcgct acggtgaccg tgaatttgac agacacctca
    29881 ctgaagcagg cacgggatta aatcgcctgt tcctgcacgc cgcagcgttg aagtttactc
    29941 atccggggac cggtgaggtg atgcgtattg aagcgccgat ggatgaaggt ttgaagcgtt
    30001 gtttgcaaaa gctgcgtaac gcgcgctaat acaagcatat aaacctgata atgggtgttg
    30061 gggttcagtt cagtttacgc cgcatcaggc cgccagcacc gattgccgga tgcgacgtaa
    30121 ccgcatccgg cgcaaacaac tatcccatca gcgggttttt cccttcccga cgtaacatct
    30181 gacacagcgc gattagcggt aaaccaacca gcgtgttagg atcacgcccc tctaaacgct
    30241 caaacagcgt aatgccaaat ccttcactct taaagctacc cgcgcagtgc agaggatgct
    30301 ccttacgcac gtaattatca atctccgcct cgctcaggtg acggaaatgg acgtcaaaag
    30361 gctccacttc tgtttgcaga tgcccattcg ccgaattaaa caacgccagt ccagtatcga
    30421 aggtgacgat attgccgctg gctttgcgta attgcagacg ggcattttct tccgttaacg
    30481 gtttaccggt gatttcgcca tcaagaacac atacctggtc tgaaccaata attaaatgat
    30541 ccggataacg tgacgccaga gattgcgctt tttcttgtgc cagtcgaagc accaactgtc
    30601 gcggtgattc gtcgctgcgt ggggtttcgt cgacctctgg tgctgcacat tcaaaagaga
    30661 tttgcagttt ttccagaaga gcgcgacgcc agggcgaagt ggaggctaaa ataagtttag
    30721 gcatattttt ttccatcaga tatagcgtat tgatgatagc cattttaaac tatgcgcttc
    30781 gttttgcagg ttgatgttcg ttatcagcac tgaacgaaaa taaagcagta acccgcaatg
    30841 tgtgcgaatt attggcaaaa ggcaaccaca ggctgccttt ttctttgact ctatgacgtt
    30901 acaaagttaa tatgcgcgcc ctatgcaaaa ggtaaaatta cccctgactc tcgatccggt
    30961 tcgtacggct caaaaacgcc ttgattacca gggtatctat actcctgatc aggttgagcg
    31021 cgtcgccgaa tccgtagtca gtgtggacag tgatgtggaa tgctccatgt cgttcgctat
    31081 cgataaccaa cgtctcgcag tgttaaacgg cgatgcgaag gtgacggtaa cgctcgagtg
    31141 tcagcgttgc gggaagccgt ttactcatca ggtctacaca acgtattgtt ttagtcctgt
    31201 gcgttcagac gaacaggctg aagcactgcc ggaagcgtat gaaccgattg aggttaacga
    31261 attcggtgaa atcgatctgc ttgcaatggt tgaagatgaa atcatcctcg ccttgccggt
    31321 agttccggtg catgattctg aacactgtga agtgtccgaa gcggacatgg tctttggtga
    31381 actgcctgaa gaagcgcaaa agccaaaccc atttgccgta ttagccagct taaagcgtaa
    31441 gtaattggtg ctccccgttg gatcggggat aaaccgtaat tgaggagtaa ggtccatggc
    31501 cgtacaacag aataaaccaa cccgttccaa acgtggcatg cgtcgttccc atgacgcgct
    31561 gaccgcagtc accagcctgt ctgtagacaa aacttctggt gaaaaacacc tgcgtcacca
    31621 catcactgcc gacggttact accgcggccg caaggtcatc gctaagtaat cacgcgtctg
    31681 cgtgatgaag cttagtgagg attttcccca ggcaactggg gaaagaccaa accgggcggc
    31741 gacgatacct tgacacgtct aaccctggcg ttagatgtca tgggagggga ttttggccct
    31801 tccgtgacag tgcctgcagc attgcaggca ctgaattcta attcgcaact cactcttctt
    31861 ttagt
//
LOCUS       DAMZFT010000048        31463 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.048, whole genome shotgun sequence.
ACCESSION   DAMZFT010000048 DAMZFT010000000
VERSION     DAMZFT010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 31463)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31463)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31463
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.048"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(<1..1308)
                     /locus_tag="PN977_002286"
     CDS             complement(<1..1308)
                     /locus_tag="PN977_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417641.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HDI6042315.1"
                     /translation="MTVFNKFARSFKSHWLLYLCVIVFGITNLVASSGAHMVQRLLFF
                     VLTILVVKRISSLPLRLLVAAPFVLLTAADMSISLYSWCTFGTTFNDGFAISVLQSDP
                     DEVVKMLGMYIPYLCAFAFLSLLFLAVIIKYDVSLPTKKVTGILLLIVISGSLFSACQ
                     FAYKDAKNKKAFSPYILASRFATYTPFFNLNYFALAAKEHQRLLSIANTVPYFQLSVR
                     DTGIDTYVLIVGESVRVDNMSLYGYTRSTTPQVEAQRKQIKLFNQAISGAPYTALSVP
                     LSLTADSVLSHDIHNYPDNIINMANQAGFQTFWLSSQSAFRQNGTAVTSIAMRAMETV
                     YVRGFDELLLPHLSQALQQKTQQKKLIVLHLNGSHEPACSAYPQSSAVFQPQDDQDAC
                     YDNSIHYTDSLLGQVFELLKDRRASVMYFADHGLERDPTKKNVY"
     gene            complement(1767..1853)
                     /locus_tag="PN977_002287"
     tRNA            complement(1767..1853)
                     /locus_tag="PN977_002287"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1817..1819),aa:Leu,seq:gag)
     gene            complement(1868..2200)
                     /gene="secG"
                     /locus_tag="PN977_002288"
     CDS             complement(1868..2200)
                     /gene="secG"
                     /locus_tag="PN977_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HDI6042316.1"
                     /translation="MYEALLVVFLIVAIGLVGLIMLQQGKGADMGASFGAGASATLFG
                     SSGSGNFMTRMTALLATLFFIISLVLGNINSNKTNKGSEWENLSAPAKTEQTQSAAPA
                     KPTSDIPN"
     gene            complement(2428..3765)
                     /gene="glmM"
                     /locus_tag="PN977_002289"
     CDS             complement(2428..3765)
                     /gene="glmM"
                     /locus_tag="PN977_002289"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HDI6042317.1"
                     /translation="MSNRKYFGTDGIRGRVGDAPITPDFVLKLGWAAGKVLARHGSRK
                     IIIGKDTRISGYMLESALEAGLAAAGLSALFTGPMPTPAVAYLTRTFRAEAGIVISAS
                     HNPFYDNGIKFFSIDGTKLPDAVEEAIEAEMEKEISCVDSAELGKASRIVDAAGRYIE
                     FCKATFPNELSLSELKIVVDCANGATYHIAPNVLRELGANVIAIGCEPNGVNINAEVG
                     ATDVRALQARVLAEKADLGIAFDGDGDRVIMVDHEGNKVDGDQIMYIIAREGLRQGQL
                     RGGAVGTLMSNMGLELALKQLGIPFARAKVGDRYVLEKMQEKGWRIGAENSGHVILLD
                     KTTTGDGIVAGLQVLAAMARNHMSLHDLCSGMKMFPQILVNVRYTAGSGDPLEHESVK
                     AVTAEVEAALGSRGRVLLRKSGTEPLIRVMVEGEDEAQVTEFAHRIADAVKAV"
     gene            complement(3758..4606)
                     /gene="folP"
                     /locus_tag="PN977_002290"
     CDS             complement(3758..4606)
                     /gene="folP"
                     /locus_tag="PN977_002290"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HDI6042318.1"
                     /translation="MKLFAQGTSLDLSHPHVMGILNVTPDSFSDGGTHNSLIDAVKHA
                     NLMINAGATIIDVGGESTRPGAAEVSVEEELQRVIPVVEAIAQRFEVWISVDTSKPEV
                     IRESAKVGAHIINDIRSLSEPGALEAAAETGLPVCLMHMQGNPKTMQEAPKYDDVFAE
                     VNRYFIEQIARCEQAGIAKEKLLLDPGFGFGKNLSHNYSLLARLAEFHHFNLPLLVGM
                     SRKSMIGQLLNVGPSERLSGSLACAVIAAMQGAHIIRVHDVKETVEAMRVVEATLSAK
                     ENKRYE"
     gene            complement(4696..6630)
                     /gene="ftsH"
                     /locus_tag="PN977_002291"
     CDS             complement(4696..6630)
                     /gene="ftsH"
                     /locus_tag="PN977_002291"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HDI6042319.1"
                     /translation="MAKNLILWLVIAVVLMSVFQSFGPSESNGRKVDYSTFLQEVNND
                     QVREARINGREINVTKKDSNRYTTYIPVQDPKLLDNLLTKNVKVVGEPPEEPSLLASI
                     FISWFPMLLLIGVWIFFMRQMQGGGGKGAMSFGKSKARMLTEDQIKTTFADVAGCDEA
                     KEEVAELVEYLREPSRFQKLGGKIPKGVLMVGPPGTGKTLLAKAIAGEAKVPFFTISG
                     SDFVEMFVGVGASRVRDMFEQAKKAAPCIIFIDEIDAVGRQRGAGLGGGHDEREQTLN
                     QMLVEMDGFEGNEGIIVIAATNRPDVLDPALLRPGRFDRQVVVGLPDVRGREQILKVH
                     MRRVPLAPDIDAAIIARGTPGFSGADLANLVNEAALFAARGNKRVVSMVEFEKAKDKI
                     MMGAERRSMVMTEAQKESTAYHEAGHAIIGRLVPEHDPVHKVTIIPRGRALGVTFFLP
                     EGDAISASRQKLESQISTLYGGRLAEEIIYGPEHVSTGASNDIKVATNLARNMVTQWG
                     FSEKLGPLLYAEEEGEVFLGRSVAKAKHMSDETARIIDQEVKALIERNYNRARQLLTD
                     NMDILHAMKDALMKYETIDAPQIDDLMARRDVRPPAGWEEPVASNNSGDNGSPKAPRP
                     VDEPRTPNPGNTMSEQLGDK"
     gene            complement(6730..7359)
                     /gene="rlmE"
                     /locus_tag="PN977_002292"
     CDS             complement(6730..7359)
                     /gene="rlmE"
                     /locus_tag="PN977_002292"
                     /EC_number="2.1.1.166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uridine(2552)-2'-O)-methyltransferase
                     RlmE"
                     /protein_id="HDI6042320.1"
                     /translation="MTGKKRSASSSRWLQEHFSDKYVQQAQKKGLRSRAWFKLDEIQQ
                     SDKLFKPGMTVVDLGAAPGGWSQYVVTQIGGKGRIIACDLLPMDPIVGVDFLQGDFRD
                     ELVMKALLERVGDSKVQVVMSDMAPNMSGTPAVDIPRAMYLVELALEMCRDVLAPGGS
                     FVVKVFQGEGFDEYLREIRSLFTKVKVRKPDSSRARSREVYIVATGRKP"
     gene            7485..7778
                     /gene="yhbY"
                     /locus_tag="PN977_002293"
     CDS             7485..7778
                     /gene="yhbY"
                     /locus_tag="PN977_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HDI6042321.1"
                     /translation="MNLSTKQKQHLKGLAHPLKPVVLLGSNGLTEGVLAEIEQALEHH
                     ELIKVKIATEDRETKTLIVEAIVRETGACNVQVIGKTLVLYRPTKERKISLPR"
     gene            complement(7934..8410)
                     /gene="greA"
                     /locus_tag="PN977_002294"
     CDS             complement(7934..8410)
                     /gene="greA"
                     /locus_tag="PN977_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006178768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HDI6042322.1"
                     /translation="MQAIPMTLRGAEKLREELDFLKSVRRPEIIAAIAEAREHGDLKE
                     NAEYHAAREQQGFCEGRIKDIEAKLSNAQVIDVTKMPNNGRVIFGATVTVLNLDSDEE
                     QTYRIVGDDEADFKQNLISVNSPIARGLIGKEEDDVVVIKTPGGEVEFEVIKVEYL"
     gene            8659..10092
                     /gene="dacB"
                     /locus_tag="PN977_002295"
     CDS             8659..10092
                     /gene="dacB"
                     /locus_tag="PN977_002295"
                     /EC_number="3.4.16.4"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001212660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HDI6042323.1"
                     /translation="MRFSRFIIGLTSCIAFSVQAANVDEYITQLPAGANLALMVQKVG
                     ASAPAIDYHSQQMALPASTQKVITALAALIQLGPDFRFTTTLETKGNVENGVLKGDLV
                     ARFGADPTLKRQDIRNMVATLKKSGVNQIDGNVLIDTSIFASHDKAPGWPWNDMTQCF
                     SAPPAAAIVDRNCFSVSLYSAPKPGDMAFIRVASYYPVTMFSQVRTLPRGSAEAQYCE
                     LDVVPGDLNRFTLTGCLPQRSEPLPLAFAVQDGASYAGAILKDELKQAGITWSGTLLR
                     QTQVNEPGTVVASKQSAPLHDLLKIMLKKSDNMIADTVFRMIGHARFNVPGTWRAGSD
                     AVRQILRQQAGVDIGNTIIADGSGLSRHNLIAPATMMQVLQYIAQHDNELNFISMLPL
                     AGYDGSLQYRAGLHQAGVDGKVSAKTGSLQGVYNLAGFITTASGQRMAFVQYLSGYAV
                     EPADQRNRRIPLVRFESRLYKDIYQNN"
     gene            complement(10133..11305)
                     /gene="cgtA"
                     /locus_tag="PN977_002296"
     CDS             complement(10133..11305)
                     /gene="cgtA"
                     /locus_tag="PN977_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Obg family GTPase CgtA"
                     /protein_id="HDI6042324.1"
                     /translation="MKFVDEASILVVAGDGGNGCVSFRREKYIPKGGPDGGDGGDGGD
                     VWMEADENLNTLIDYRFEKSFRAERGQNGASRDCTGKRGKDVTIKVPVGTRVIDQGTG
                     ETMGDMTKHGQRLLVAKGGWHGLGNTRFKSSVNRTPRQKTNGTPGDKRELLLELMLLA
                     DVGMLGMPNAGKSTFIRAVSAAKPKVADYPFTTLVPSLGVVRMDNEKSFVVADIPGLI
                     EGAAEGAGLGIRFLKHLERCRVLLHLIDIDPIDGTDPVENARIIISELEKYSQDLAAK
                     PRWLVFNKIDLLDKAEAEEKAKAIAEALGWEDKYYLISAASGLGVKDLCWDVMTFIIE
                     NPVVQAEEAKQPEKVEFMWDDYHRQQLEEIAEEDDEDWDDDWDEDDEEGVEFIYKR"
     gene            complement(11321..12286)
                     /locus_tag="PN977_002297"
     CDS             complement(11321..12286)
                     /locus_tag="PN977_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012135318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HDI6042325.1"
                     /translation="MKQQAGIGILLALTTAICWGALPIAMKQVLEVMEPPTIVFYRFL
                     MASIGLGAILAVKKRLPPLRVFRKPRWLILLAVATAGLFGNFILFSSSLQYLSPTASQ
                     VIGQLSPVGMMVASVFILKEKMRSTQVVGALMLLSGLVMFFNTSLVEIFTKLTDYTWG
                     VIFGVGAATVWVSYGVAQKVLLRRLASPQILFLLYTLCTIALFPLAKPGVIAQLSHWQ
                     LACLIFCGLNTLVGYGALAEAMARWQAAQVSAIITLTPLFTLFFSDLLSLAWPDFFAR
                     PMLNLLGYLGAFVVVAGAMYSAIGHRIWGGLRKHTTVVSQPRAGE"
     gene            complement(12413..12670)
                     /gene="rpmA"
                     /locus_tag="PN977_002298"
     CDS             complement(12413..12670)
                     /gene="rpmA"
                     /locus_tag="PN977_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HDI6042326.1"
                     /translation="MAHKKAGGSTRNGRDSEAKRLGVKRFGGESVLAGSIIVRQRGTK
                     FHAGANVGCGRDHTLFAKADGKVKFEVKGPKNRKFISIEAE"
     gene            complement(12691..13002)
                     /gene="rplU"
                     /locus_tag="PN977_002299"
     CDS             complement(12691..13002)
                     /gene="rplU"
                     /locus_tag="PN977_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015960603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HDI6042327.1"
                     /translation="MYAVFQSGGKQHRVSEGQTVRLEKLDIATGETVEFAEVLMIANG
                     EEVKIGVPFVDGGVIKAEVVAHGRGEKVKIVKFRRRKHYRKQQGHRQWFTDVKITGIS
                     A"
     gene            13261..14232
                     /gene="ispB"
                     /locus_tag="PN977_002300"
     CDS             13261..14232
                     /gene="ispB"
                     /locus_tag="PN977_002300"
                     /EC_number="2.5.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="octaprenyl diphosphate synthase"
                     /protein_id="HDI6042328.1"
                     /translation="MNLEKINELTAQDMAGVNAAILEQLNSDVQLINQLGYYIVSGGG
                     KRIRPMIAVLAARAVGYEGNAHVTIAALIEFIHTATLLHDDVVDESDMRRGKATANAA
                     FGNAASVLVGDFIYTRAFQMMTSLGSLKVLEVMSEAVNVIAEGEVLQLMNVNDPDITE
                     ENYMRVIYSKTARLFEAAAQCSGILAGCTPEEEKGLQDYGRYLGTAFQLIDDLLDYNA
                     DGEQLGKNIGDDLNEGKPTLPLLHAMHHGTPEQAQMIRTAIEQGNGRHLLEPVLEAMN
                     ACGSLEWTRQRAEEEADKAIAALQVLPDTPWREALIGLAHIAVQRDR"
     gene            14461..14739
                     /gene="sfsB"
                     /locus_tag="PN977_002301"
     CDS             14461..14739
                     /gene="sfsB"
                     /locus_tag="PN977_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SfsB"
                     /protein_id="HDI6042329.1"
                     /translation="MESNFIDWHPADIIAGLRKKGTSMAAESRRNGLSSSTLANALSR
                     PWPKGEMIIAKALGTDPWIIWPSRYHDPQTHEFIDRTQLMRSYTKPKK"
     gene            complement(14787..16046)
                     /gene="murA"
                     /locus_tag="PN977_002302"
     CDS             complement(14787..16046)
                     /gene="murA"
                     /locus_tag="PN977_002302"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HDI6042330.1"
                     /translation="MDKFRVQGPTKLQGEVTISGAKNAALPILFAALLAEEPVEIQNV
                     PKLKDVDTSMKLLSQLGAKVERNGSVHIDARDVNVFCAPYDLVKTMRASIWALGPLVA
                     RFGQGQVSLPGGCTIGARPVDLHISGLEQLGATIKLEEGYVKASVDGRLKGAHIVMDK
                     VSVGATVTIMCAATLAEGTTIIENAAREPEIVDTANFLITLGAKISGQGTDRIVIEGV
                     ERLGGGVYRVLPDRIETGTFLVAAAISRGKIICRNAQPDTLDAVLAKLRDAGADIEVG
                     EDWISLDMHGKRPKAVNVRTAPHPAFPTDMQAQFTLLNLVAEGTGFITETVFENRFMH
                     VPELSRMGAHAEIESNTVICHGVEKLSGAQVMATDLRASASLVLAGCIAEGTTVVDRI
                     YHIDRGYERIEDKLRALGANIERVKGE"
     gene            complement(16101..16355)
                     /gene="ibaG"
                     /locus_tag="PN977_002303"
     CDS             complement(16101..16355)
                     /gene="ibaG"
                     /locus_tag="PN977_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family iron metabolism protein IbaG"
                     /protein_id="HDI6042331.1"
                     /translation="MENNEIQSVLMNALSLQEVHVSGDGSHFQVIAVGELFDGMSRVK
                     KQQTVYGPLMEYIADNRIHAVSIKAYTPAEWARDRKLNGF"
     gene            complement(16515..16808)
                     /gene="mlaB"
                     /locus_tag="PN977_002304"
     CDS             complement(16515..16808)
                     /gene="mlaB"
                     /locus_tag="PN977_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance protein MlaB"
                     /protein_id="HDI6042332.1"
                     /translation="MSESLSWMQTGDTLALSGELDQDVLLPLWEMREEAVKGITCIDL
                     SRVSRVDTGGLALLLHLIDLAKKQGNNVTLQGVNDKVYTLAKLYNLPADVLPR"
     gene            complement(16808..17443)
                     /gene="mlaC"
                     /locus_tag="PN977_002305"
     CDS             complement(16808..17443)
                     /gene="mlaC"
                     /locus_tag="PN977_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding protein MlaC"
                     /protein_id="HDI6042333.1"
                     /translation="MFKRLMMVALLVIAPLSAATAADQTNPYKLMDEAAQKTFDRLKN
                     EQPQIRANPDYLRTIVDQELLPYVQVKYAGALVLGQYYKSATPAQRDAYFAAFREYLK
                     QAYGQALAMYHGQTYQIAPEQPLGDKTIVPIRVTIIDPNGRPPVRLDFQWRKNSQTGN
                     WQAYDMIAEGVSMITTKQNEWGTLLRTKGIDGLTAQLKSISQQKITLEEKK"
     gene            complement(17462..18013)
                     /gene="mlaD"
                     /locus_tag="PN977_002306"
     CDS             complement(17462..18013)
                     /gene="mlaD"
                     /locus_tag="PN977_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HDI6042334.1"
                     /translation="MQTKKNEIWVGIFLLAALLAALFVCLKAANVTSIRTEPTYTLYA
                     TFDNIGGLKARSPVSIGGVVVGRVADITLDPKTYLPRVTLEIEQRYNHIPDTSSLSIR
                     TSGLLGEQYLALNVGFEDPELGTAILKDGDTIQDTKSAMVLEDLIGQFLYGSKGDDNK
                     NSGDAPAAAPGNNETTEPVGTTK"
     gene            complement(18018..18800)
                     /gene="mlaE"
                     /locus_tag="PN977_002307"
     CDS             complement(18018..18800)
                     /gene="mlaE"
                     /locus_tag="PN977_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014071945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance ABC transporter
                     permease subunit MlaE"
                     /protein_id="HDI6042335.1"
                     /translation="MLLNALASLGHKGIKTLRTFGRAGLMLFNALVGKPEFRKHAPLL
                     VRQLYNVGVLSMLIIVVSGVFIGMVLGLQGYLVLTTYSAETSLGMLVALSLLRELGPV
                     VAALLFAGRAGSALTAEIGLMRATEQLSSMEMMAVDPLRRVISPRFWAGVISLPLLTV
                     IFVAVGIWGGSLVGVSWKGIDSGFFWSAMQNAVDWRMDLVNCLIKSVVFAITVTWISL
                     FNGYDAIPTSAGISRATTRTVVHSSLAVLGLDFVLTALMFGN"
     gene            complement(18808..19617)
                     /gene="mlaF"
                     /locus_tag="PN977_002308"
     CDS             complement(18808..19617)
                     /gene="mlaF"
                     /locus_tag="PN977_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid ABC transporter ATP-binding protein
                     MlaF"
                     /protein_id="HDI6042336.1"
                     /translation="MEQSVANLVDMRDVSFTRGNRCIFDNISLTVPRGKITAIMGPSG
                     IGKTTLLRLIGGQIAPDHGEILFDGENIPAMSRSRLYTVRKRMSMLFQSGALFTDMNV
                     FDNVAYPLREHTQLPAPLLHSTVMMKLEAVGLRGAAKLMPSELSGGMARRAALARAIA
                     LEPDLIMFDEPFVGQDPITMGVLVKLISELNSALGVTCVVVSHDVPEVLSIADHAWIL
                     ADKKIVAHGSAQALQANPDPRVRQFLDGIADGPVSFRYPAGDYHADLLPGS"
     gene            19827..20804
                     /locus_tag="PN977_002309"
     CDS             19827..20804
                     /locus_tag="PN977_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HDI6042337.1"
                     /translation="MLLATALLIVGLLLVVYSADRLVFAASILCRTFGIPPLIIGMTV
                     VSIGTSLPEIIVSLAASLHEQRDLAVGTALGSNIINILLILGLAALVRPFTVHSDVLR
                     RELPLMLLVSVVAGSVLYDGQLSRSDGIFLLFLAVLWLLFIVKLARQAERQGTDSLTR
                     EQLAELPRDGGLPVAFLWLGIALIIMPVATRMVVDNATVLANYFAISELTMGLTAIAI
                     GTSLPELATAIAGVRKGENDIAVGNIIGANIFNIVIVLGLPALITPGEIDPLAYSRDY
                     SVMLLVSIIFALLCWRRSPQSGRGVGVLLTGGFIVWLAMLYWLSPILVE"
     gene            20818..21804
                     /gene="kdsD"
                     /locus_tag="PN977_002310"
     CDS             20818..21804
                     /gene="kdsD"
                     /locus_tag="PN977_002310"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase KdsD"
                     /protein_id="HDI6042338.1"
                     /translation="MSHVELQPGFDFQQAGKEVLAIERECLAELDQYINQNFTLACEK
                     MFWCKGKVVVMGMGKSGHIGRKMAATFASTGTPSFFVHPGEAAHGDLGMVTPQDVVIA
                     ISNSGESSEITALIPVLKRLHVPLICITGRPESSMARAADVHLCVKVAKEACPLGLAP
                     TSSTTATLVMGDALAVALLKARGFTAEDFALSHPGGALGRKLLLRVNDIMHTGDEIPH
                     VKKTASLRDALLEVTRKNLGMTVICDDNMMIEGIFTDGDLRRVFDMGVDVRQLSIADV
                     MTPGGIRVRPGILAVEALNLMQSRHITSVMVADGDHLLGVLHMHDLLRAGVV"
     gene            21825..22391
                     /gene="kdsC"
                     /locus_tag="PN977_002311"
     CDS             21825..22391
                     /gene="kdsC"
                     /locus_tag="PN977_002311"
                     /EC_number="3.1.3.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate-8-phosphatase KdsC"
                     /protein_id="HDI6042339.1"
                     /translation="MSKAGASLATCYGPVSADVMAKAENIRLLILDVDGVLSDGLIYM
                     GNNGEELKAFNVRDGYGIRCALTSDIEVAIITGRKAKLVEDRCATLGITHLYQGQSNK
                     LIAFSDLLEKLAIAPENVAYVGDDLIDWPVMEKVGLSVAVADAHPLLIPRADYVTRIA
                     GGRGAVREVCDLLLLAQGKLDEAKGQSI"
     gene            22388..22963
                     /gene="lptC"
                     /locus_tag="PN977_002312"
     CDS             22388..22963
                     /gene="lptC"
                     /locus_tag="PN977_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HDI6042340.1"
                     /translation="MSKARRWVIIVLSLAVLVMIGINMAEKDDTAQVVVNNNDPTYKS
                     EHTDTLVYNPEGALSYRLIAQHVEYYSDQAVSWFTQPVLTTFDKDKIPTWSVKADKAK
                     LTNDRMLYLYGHVEVNALVPDSQLRRITTDNAQINLVTQDVTSEDLVTLYGTTFNSSG
                     LKMRGNLRSKNAELIEKVRTSYEIQNKQTQP"
     gene            22932..23489
                     /gene="lptA"
                     /locus_tag="PN977_002313"
     CDS             22932..23489
                     /gene="lptA"
                     /locus_tag="PN977_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide ABC transporter
                     substrate-binding protein LptA"
                     /protein_id="HDI6042341.1"
                     /translation="MKFKTNKLSLNLVLASSLLAASIPAFAVTGDTDQPIHIESDQQS
                     LDMQGNVVTFTGNVIVTQGTIKINADKVVVTRPGGEQGKEVIDGYGKPATFYQMQDNG
                     KPVEGHASQMHYELAKDFVVLTGNAYLQQVDSNIKGDKITYLVKEQKMQAFSDKGKRV
                     TTVLVPSQLQDKNNKGQTPAQKKGN"
     gene            23496..24221
                     /gene="lptB"
                     /locus_tag="PN977_002314"
     CDS             23496..24221
                     /gene="lptB"
                     /locus_tag="PN977_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HDI6042342.1"
                     /translation="MATLTAKNLAKAYKGRRVVEDVSLTVNSGEIVGLLGPNGAGKTT
                     TFYMVVGIVPRDAGNIIIDDDDISLLPLHARARRGIGYLPQEASIFRRLSVYDNLMAV
                     LQIRDDLSAEQREDRANELMEEFHIEHLRDSMGQSLSGGERRRVEIARALAANPKFIL
                     LDEPFAGVDPISVIDIKRIIEHLRDSGLGVLITDHNVRETLAVCERAYIVSQGHLIAH
                     GTPTEILQDEHVKRVYLGEDFRL"
     gene            24269..25702
                     /gene="rpoN"
                     /locus_tag="PN977_002315"
     CDS             24269..25702
                     /gene="rpoN"
                     /locus_tag="PN977_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HDI6042343.1"
                     /translation="MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALESN
                     PLLEQIDTHEEIDTRETQDSETLDTADALEQKEMPEELPLDASWDTIYTAGTPSGTSG
                     DYIDDELPVYQGETTQTLQDYLMWQVELTPFSDTDRAIATSIVDAVDDTGYLTVPLED
                     ILESMGDEEIDIDEVEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFDKTTPWLEEARL
                     IISDHLDLLANHDFRTLMRVTRLKEDVLKEAVNLIQSLDPRPGQSIQTGEPEYVIPDV
                     LVRKHNGHWTVELNSDSIPRLQINQHYASMCNNARNDGDSQFIRSNLQDAKWLIKSLE
                     SRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHS
                     PRGIFELKYFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTSLLSEQG
                     IMVARRTVAKYRESLSIPPSNQRKQLV"
     gene            25725..26012
                     /gene="hpf"
                     /locus_tag="PN977_002316"
     CDS             25725..26012
                     /gene="hpf"
                     /locus_tag="PN977_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome hibernation promoting factor"
                     /protein_id="HDI6042344.1"
                     /translation="MQLNITGNNVEITEALREFVTAKFAKLEQYFDRINQVYVVLKVE
                     KVTHTSDATLHVNGGEIHASAEGQDMYAAIDGLIDKLARQLTKHKDKLKQH"
     gene            26130..26621
                     /gene="ptsN"
                     /locus_tag="PN977_002317"
     CDS             26130..26621
                     /gene="ptsN"
                     /locus_tag="PN977_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS IIA-like nitrogen regulatory protein PtsN"
                     /protein_id="HDI6042345.1"
                     /translation="MTNNDTTLQLSSVLNRECTRSRVHCQSKKRALEIISELAAKQLS
                     LPPQVVFEAILTREKMGSTGIGNGIAIPHGKLEEDTLRAVGVFVQLETPIAFDAIDNQ
                     PVDLLFALLVPADQTKTHLHTLSLVAKRLADKTICRRLRAAQSDEELYQIITDTEGTP
                     DEA"
     gene            26667..27521
                     /gene="rapZ"
                     /locus_tag="PN977_002318"
     CDS             26667..27521
                     /gene="rapZ"
                     /locus_tag="PN977_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HDI6042346.1"
                     /translation="MVLMIVSGRSGSGKSVALRALEDMGFYCVDNLPVVLLPDLARTL
                     ADREISAAVSIDVRNMPESPEIFEQAMSNLPDAFSPQLLFLDADRNTLIRRYSDTRRL
                     HPLSSKNLSLESAIDKESDLLEPLRSRADLIVDTSEMSVHELAEMLRTRLLGKREREL
                     TMVFESFGFKHGIPIDADYVFDVRFLPNPHWDPKLRPMTGLDKPVAAFLDRHTEVHNF
                     IYQTRSYLELWLPMLETNNRSYLTVAIGCTGGKHRSVYIAEQLADYFRSRGKNVQSRH
                     RTLEKRKP"
     gene            27518..27790
                     /gene="npr"
                     /locus_tag="PN977_002319"
     CDS             27518..27790
                     /gene="npr"
                     /locus_tag="PN977_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000216792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS phosphocarrier protein NPr"
                     /protein_id="HDI6042347.1"
                     /translation="MTVKQTVEITNKLGMHARPAMKLFELMQGFDAEVLLRNDEGTEA
                     EANSVIALLMLDSAKGRQIEVEATGPQEEEALAAVIALFNSGFDED"
     gene            28004..28636
                     /gene="yrbL"
                     /locus_tag="PN977_002320"
     CDS             28004..28636
                     /gene="yrbL"
                     /locus_tag="PN977_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP regulatory network protein YrbL"
                     /protein_id="HDI6042348.1"
                     /translation="MIRLSEQSPLGTGRHRKCYAHPEDAQRCIKIVYHRGDGGDKEIR
                     RELKYYAHLGRRLKDWSGIPRYHGTVETDCGTGYVYDVIADFDGKPSITLTEFAEQCR
                     YEEDIAQLRQLLKQLKRYLQDNRIVTMSLKPQNILCHRISESEVIPVVCDNIGESTLI
                     PLATWSKWCCLRKQERLWKRFIAQPALAIALQKDLQPRESKTLALTSREA"
     gene            complement(28633..29361)
                     /gene="mtgA"
                     /locus_tag="PN977_002321"
     CDS             complement(28633..29361)
                     /gene="mtgA"
                     /locus_tag="PN977_002321"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HDI6042349.1"
                     /translation="MSKSRLTVFSFVRRFLLRLMVVLAVFWGGGIALFSVAPVPFSAV
                     MVERQVSAWLHGNFRYVAHSDWVSMDQISPWMGLAVIAAEDQKFPEHWGFDVASIEQA
                     LAHNERNENRIRGASTISQQTAKNLFLWDGRSWVRKGLEAGLTLGIETVWSKKRILTV
                     YLNIAEFGDGVFGVEAAAQRYFHKPASKLTRSEAALLAAVLPNPLRFKVSAPSGYVRS
                     RQAWILRQMYQLGGEPFMQQHQLD"
     gene            complement(29358..30011)
                     /gene="elbB"
                     /locus_tag="PN977_002322"
     CDS             complement(29358..30011)
                     /gene="elbB"
                     /locus_tag="PN977_002322"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenoid biosynthesis glyoxalase ElbB"
                     /protein_id="HDI6042350.1"
                     /translation="MKKIGVILSGCGVYDGSEIHEAVLTLLAISRSGAQAVCFAPDKQ
                     QVDVINHLTGEAMPETRNVLIEAARITRGEIRPLAQADAAELDALIVPGGFGAAKNLS
                     NFARLGSECTVDRELKALAQAMHQAGKPLGFMCIAPAMLPKIFDFPLRLTIGTDIDTA
                     EVLEEMGAEHVPCPVDDIVVDEDNKIVTTPAYMLAQNIAEAASGIDKLVSRVLVLAE"
     gene            complement(30241..>31463)
                     /locus_tag="PN977_002323"
     CDS             complement(30241..>31463)
                     /locus_tag="PN977_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HDI6042351.1"
                     /translation="AQQKGLRFNLEPTLPLPHQVITDGTRLRQILWNLISNAVKFTQQ
                     GQVTVRVRYDEGDMLHFEVEDSGIGIPQDELDKIFAMYYQVKDSHGGKPATGTGIGLA
                     VSRRLAKNMGGDITVTSEQGKGSTFTLTIHAPSVAEEVDDAFDEDDMPLPALNVLLVE
                     DIELNVIVARSVLEKLGNSVDVAMTGKAALEMFKPGEYDLVLLDIQLPDMTGLDISRE
                     LTKRYPREDLPPLVALTANVLKDKQEYLNAGMDDVLSKPLSVPALTAMIKKFWDTQDD
                     EESTVTTEENSKSEALLDIPMLEQYLELVGPKLITDGLAVFEKMMPGYVSVLESNLTA
                     QDKKGIVEEGHKIKGAAGSVGLRHLQQLGQQIQSPDLPAWEDNVGEWIEEMKEEWRHD
                     VEVLKAWVAKATKK"
BASE COUNT         7903 a         8443 c         7572 g         7545 t
ORIGIN      
        1 atagacgttc ttcttcgtag ggtcgcgttc caggccgtgg tcggcaaaat acatgaccga
       61 ggcgcggcga tcttttaata attcaaaaac ctgacctagc aaactatctg tgtaatgaat
      121 ggagttgtca tagcaggcat cctgatcgtc ctgcggttga aacacggcgc tggattgcgg
      181 ataggcgcta caagccggtt catggcttcc atttaaatga agaacaatca gtttcttttg
      241 ctgcgtcttt tgctgtaacg cttgcgataa atgcggcaac aacaattcat caaatcctct
      301 gacatagact gtttccatgg cgcgcatggc gatactggta actgctgtac cattctgccg
      361 aaaagcggat tgcgagctta gccagaaagt ctgaaatcct gcttgattag ccatattaat
      421 aatgttgtcc gggtaattat gaatgtcatg actcaaaaca gaatcagcag ttaaagaaag
      481 gggaaccgac agcgcagtgt aaggtgcacc gcttattgct tgattaaaca gtttgatctg
      541 ttttctttgt gcttcgactt gcggtgtcgt agagcgtgtg tatccataca aagacatatt
      601 gtcgacacgt acagactccc ccacaatcaa cacgtaggta tcaatacctg tatccctgac
      661 tgataattga aaatacggca ccgtgtttgc aattgagagt aatctttgat gctctttcgc
      721 tgctaaagca aaataattga gattgaaaaa cggtgtatag gtagcaaatc gagacgctag
      781 tatatatgga ctgaacgctt ttttattttt tgcatcttta taagcaaatt gacaagctga
      841 aaataaactg cccgaaatga caatcagcaa taatattcct gtcacttttt ttgtcggcaa
      901 ggaaacatca tattttatta ttactgccaa aaaaagaagg gataaaaaag caaaagcaca
      961 tagataaggg atatacatcc ccaacatttt cacaacttca tccggatcac tctgcagcac
     1021 actaatcgca aatccatcat tgaaagttgt accaaaggta caccatgaat agaggctaat
     1081 actcatgtct gccgcagtca gtaacacaaa tggtgcggca acaagcaggc gaagcggaag
     1141 ggatgagata cgtttaacaa ccaggatggt caggacgaag aacagcaatc gctgaaccat
     1201 atgcgctccg gaagaggcga ctaagttcgt aataccaaaa acaataacac aaagatacaa
     1261 caaccaatga gatttaaaac ttctagcaaa tttgttgaat actgtcatcc agaaatccag
     1321 gttcacaacc aatataaaca attatcaaca atgttcaatt ttatatcaga catgtttttc
     1381 aactctgatc catatcagac cgaaacactg ttagtttttt ctttttaaat gatgcgacca
     1441 ttgcgttctc agtaattaaa taacattatc aatgcgtctc taaacattac gcagaacgta
     1501 gccatcagta gttatactga caggagcagg atggtttatc gagaagtaaa catatccgta
     1561 ttcgtaccgg aactggagtt gacacaataa agtgccaatt atgtcagcag aagggaaaag
     1621 tttataacta aggcgtaccg gcaccatcgt ttcaaggtac cagctacgag taaagcaact
     1681 ggacgagata cagatacctg acaaccattc ctcagaccag gaccaaaacg aaaaaagacg
     1741 cttttcagcg tctcttttct ggaatttggt accgaggacg ggacttgaac ccgtaagccc
     1801 tattgggcac taccacctca aggtagcgtg tctaccaatt ccaccacctc ggcacggata
     1861 ctacttttta gttcgggata tcgctggtcg gcttagccgg agcagctgac tgagtttgtt
     1921 cggttttcgc cggcgcactc aggttttccc attcgctacc tttattggtt ttattgctat
     1981 tgatgttacc cagcaccaga ctgatgatga agaataacgt tgccagcagc gccgtcatgc
     2041 gggtcatgaa gttaccagaa ccacttgaac caaacagcgt agcggaagcg cctgctccga
     2101 aggaggctcc catatcagcg cctttacctt gctgcagcat gatcagacca acaaggccaa
     2161 ttgccacaat aaggaaaact actaaaagag cttcgtacat aatcaacctg ttccttgcgg
     2221 agttgccgcg taccaatgct tcaaccaata aagcgggagt ttttaatctt tcccactgaa
     2281 gcgggtgtga atactaacca aagcgaatga ccttcgcaag ggcaatttta tcgcattgta
     2341 tcaactgcgg aaaaaaacag caaaaccgat gtgttcgctg agaaaaaagg cgacaatcgc
     2401 cgccttttta gccagttatc taacgcttta aacggctttt actgcatcgg cgatgcggtg
     2461 tgcaaattca gtcacctgcg cttcgtcttc gccttccacc atcacgcgaa ttaacggttc
     2521 ggtgccggat ttacgcaaca gcacacgtcc acggctaccc agcgccgctt caacttctgc
     2581 ggtcacagct ttgactgatt catgctccag cggatcgccg ctacctgcgg tgtaacgcac
     2641 gtttaccagg atttgcggga acattttcat gccgctgcaa aggtcgtgca ggctcatatg
     2701 gttacgtgcc atcgccgcca gcacctgcaa gccagcaacg atgccgtcac cggtagtggt
     2761 tttatccagc aggatcacat gaccggagtt ctctgcaccg atacgccagc ctttctcctg
     2821 cattttttcc agtacgtagc ggtcacccac tttcgcgcgc gcaaatggaa ttcctaactg
     2881 tttcagcgcc agttcaagcc ccatgttgct catcaatgta cccacagcgc caccacgcag
     2941 ctggccctga cgaagacctt cacgcgcgat gatatacatg atctgatcgc catcgacttt
     3001 attgccttca tggtcaacca taatcacgcg atcgccgtcg ccgtcgaagg caataccgag
     3061 atccgctttt tcagccagca cacgagcctg gagcgcgcga acgtcggtag cccccacttc
     3121 ggcattgata tttacaccgt tcggctcaca accgatagcg ataacgttcg cccccagttc
     3181 acgcagcacg ttcggcgcga tgtggtaagt cgcaccgttt gcacaatcca ccacaatctt
     3241 cagttcactg aggctaagtt cgttcgggaa cgtggctttg caaaactcga tatagcgacc
     3301 cgcagcatca acgatacggc tggctttacc cagttctgcc gaatcaacac agctgatctc
     3361 tttttccatt tccgcttcga tggcttcttc aactgcatcc ggtagtttgg tgccgtcgat
     3421 agagaaaaat ttaatgccat tatcgtagaa cgggttatgc gatgcagata tcacaattcc
     3481 ggcctctgcg cggaaggtac gcgtcagata agccacggcc ggtgttggca tcgggccagt
     3541 gaagagtgcg gaaagaccgg cagccgccag acccgcttcc agtgctgact ccagcatata
     3601 gccagaaata cgcgtgtctt taccaataat gatcttacgg gagccgtggc gcgccagcac
     3661 tttacccgct gcccaaccca gcttaagcac aaaatcaggt gtgatcggcg catcccctac
     3721 acgaccacga atcccatcgg taccgaaata tttacgatta ctcatagcgt ttgttttcct
     3781 ttgcagacag agtggcttcc accacccgca tcgcttctac ggtttctttg acgtcatgaa
     3841 cacgaatgat gtgcgcgcct tgcattgcgg caataaccgc acaggccaga ctaccgctca
     3901 ggcgctcgga cggccccacg ttcagcagtt gcccaatcat cgattttcgt gacataccca
     3961 ccaacagcgg caggttgaaa tggtgaaatt cagccaggcg cgccagtaat gaatagttat
     4021 gggagagatt tttaccgaaa ccgaatccgg ggtcgagcaa caatttctct tttgcgatac
     4081 ccgcctgctc gcaacgtgct atttgctcaa taaagtagcg attcacttct gcaaagacat
     4141 cgtcatactt cggcgcttcc tgcatggttt ttggatttcc ctgcatatgc atcagacaaa
     4201 ccggtaaacc ggtttctgca gccgcctcca gagcgccagg ttcggaaagg gagcggatat
     4261 cgttaataat gtgagcgcca actttcgctg actcacggat gacttctggt ttggatgtat
     4321 ctaccgagat ccagacttcg aagcgttgag caattgcctc aaccacagga ataacacgtt
     4381 gcaactcttc ttcaacgcta acttccgccg ctcctgggcg cgtggactcg ccaccaacgt
     4441 caatgatcgt cgcgccagcg ttgatcatca gattcgcatg tttcacggca tctatcagcg
     4501 agttatgcgt gccgccatcc gaaaaggaat caggcgtgac gttgaggatc cccattacgt
     4561 gaggatggct aaggtccagt gaagtgccct gggcaaagag tttcatggtg ttatccctgg
     4621 tatgaattga taagaaaaaa ccccggagca cgccccgggg ttttcggtac aaatacagtc
     4681 atctgatgcg ggaacttact tgtcgcctaa ctgctctgac atggtgttac ccgggttcgg
     4741 cgtacgcggt tcatcaaccg gacgtggagc ctttggacta ccattgtcgc cagaattgtt
     4801 agaagcgact ggttcttccc agcccgctgg cggacgtaca tcgcgacgtg ccatcaggtc
     4861 atcaatctgc ggtgcgtcga tagtctcata tttcatgaga gcatctttca tcgcatgcag
     4921 aatatccata ttgtcggtca gaagctgacg cgcacgatta tagttacgct caatcagtgc
     4981 tttcacttcc tggtcgatga tacgtgcagt ttcatcggac atatgtttcg ctttcgctac
     5041 gctacggccg aggaacactt caccttcttc ttccgcatac agcagcggac cgagtttctc
     5101 agagaaaccc cactgagtca ccatgttacg tgccaggttg gtcgcaactt taatatcgtt
     5161 ggacgcaccg gtagaaacat gttccggccc gtagatgatc tcttctgcca gacgaccacc
     5221 gtacagcgta gaaatctggc tttccagttt ctgacggctg gcgctgattg cgtcgccctc
     5281 aggcaagaag aaggtcacac ccagcgcacg accgcgcggg ataatcgtta ctttgtgcac
     5341 cggatcgtgt tccggcacca ggcgaccgat aatcgcgtga cccgcttcgt ggtaagccgt
     5401 cgattctttc tgcgcttccg tcatcaccat ggagcgacgt tccgcaccca tcatgatttt
     5461 gtctttcgct ttctcgaact caaccatcga cacaacgcgt ttgttgccac gagcagcgaa
     5521 cagtgccgct tcgttcacca ggttcgccag gtcagcaccg gagaaaccag gagtaccacg
     5581 ggcaatgatt gccgcgtcga tatcgggtgc caatggtacg cgacgcatgt gaactttcag
     5641 gatctgctca cgaccacgaa catctggcaa gccaaccaca acctgacggt cgaaacggcc
     5701 aggacgcagc agggccgggt caagaacgtc cggacggtta gtcgcggcga taacgatgat
     5761 accttcgtta ccttcgaagc catccatctc aaccagcatc tggttcaaag tctgttcacg
     5821 ttcatcgtga ccaccgccca gacctgcgcc acgctggcgg cctacggcgt cgatttcatc
     5881 gataaagatg atgcacggtg ccgctttctt cgcctgttcg aacatgtcac gaacacggga
     5941 tgcacccaca ccgacgaaca tttctacgaa gtcagaaccg gagatagtaa agaacggaac
     6001 tttcgcttcg cctgcaatcg ctttcgccag cagcgtttta ccggtacccg gaggaccgac
     6061 catcaggacg cctttcggga tcttaccgcc gagtttctgg aagcggctcg gctcgcgcag
     6121 atactcaacc agttcagcaa cttcttcttt tgcttcgtcg cagcccgcaa cgtcagcaaa
     6181 ggtcgttttg atctgatctt ccgtcagcat gcgcgctttg ctcttaccaa acgacatggc
     6241 acctttgcca ccgccgccct gcatttgacg catgaagaag atccagacac caatcagcaa
     6301 cagcatcggg aaccaggaga tgaagataga agccagcagg cttggttctt caggcggttc
     6361 accgacaacc ttgacgttct tggtcaacag gttatccagt aatttcggat cctgaaccgg
     6421 aatgtaagtg gtataacggt tactatcttt cttggtaacg ttgatttcac gtccgttgat
     6481 acgcgcttca cgaacctggt cgttattgac ctcttgtagg aaggtagagt aatccacctt
     6541 acggccatta gactcgctgg gcccaaagct ctggaatact gacatcagca caacggcaat
     6601 gaccagccag agtattaggt ttttcgccat gtcactcaag ggattaacct cttattacaa
     6661 ctgtgttaaa aacagcgtca ggatactcta tatccagcat ctttcaaact ttcgtctgaa
     6721 atctcccggt tagggtttac gcccggtcgc tacaatatac acttcccgtg aacgtgcacg
     6781 agaagagtcc ggcttacgaa ctttgacctt cgtaaacagg gagcgaattt cccttagata
     6841 ctcatcgaaa ccttcgccct ggaacacctt cactacaaaa ctgccacctg gcgctaatac
     6901 atcacgacac atttctagcg ccagttccac cagatacatg gcacggggga tatccaccgc
     6961 cggtgttccg ctcatgtttg gtgccatatc ggacatgaca acctggactt tgctgtcgcc
     7021 tacgcgctcc agcagtgctt tcatcaccag ttcatcacga aaatcgccct gaagaaagtc
     7081 cacaccaacg ataggatcca taggtaaaag atcgcaagcg atgatacggc ctttgccgcc
     7141 aatttgggtg accacatatt gtgaccaacc acctggcgca gcaccaaggt cgacaaccgt
     7201 cattcccggt ttaaagagtt tgtcactttg ctgtatttca tcaagtttaa accaggcacg
     7261 ggaacgtaac ccctttttct gtgcctgttg aacatattta tcgctaaagt gttcctgaag
     7321 ccagcggctg gagctggcag aacgcttctt acctgtcatt taactttccc atggggataa
     7381 ctcatcgtaa ccaattgcgt aaatttttac gcgcctattt ggtgatatat gggagatggc
     7441 ggtagaatga cccgttttca atcccaacgt aagcaaaaaa tacgatgaat ctgagtacta
     7501 aacaaaaaca gcacctgaaa ggtctggcac atccgctcaa gccagttgtt ctgcttggca
     7561 gtaatggttt gaccgaaggg gtgctggccg agattgaaca agcgttagag caccatgaac
     7621 tcatcaaggt gaaaatcgcc accgaagatc gcgaaactaa aaccttgatc gtggaagcta
     7681 tcgtgcgcga aaccggcgcc tgtaatgtac aggtcatcgg taaaacgctg gtgctttatc
     7741 gcccaactaa agaacgtaaa atctcgctgc cacgctaaga ttatcctaaa gttacacaca
     7801 ttcgctgtgt aaaacgaggg tttttccgca ggcaggagag caaaatgcca cgctctgttc
     7861 gttgataaaa ggccgcatag cggccttttt cctttcttta caatacatca acatcttgag
     7921 tattgggtaa ttcttacagg tattccacct taattacttc aaattctact tcgccgcccg
     7981 gcgttttgat gaccacaaca tcatcttctt ctttgccgat caggccacga gcaataggcg
     8041 agtttacaga aatcaggttt tgtttaaagt cagcttcgtc atcgccaacg atgcgataag
     8101 tctgttcttc gtcagaatcg agattcagca ccgttacggt agcaccaaaa ataacgcgcc
     8161 cattgttggg cattttggtg acatcaatca cctgcgcgtt cgacagcttg gcttcgatgt
     8221 ctttgatacg gccttcgcaa aagccctgct gttcacgagc tgcgtggtat tcggcgtttt
     8281 ctttcaggtc gccatgctca cgcgcttccg cgatagcagc aatgatttca ggacggcgca
     8341 cagatttcag aaaatccagc tcttcgcgta atttttcagc gccgcgtaag gtcatcggaa
     8401 tagcttgcat ttgttatacc tcttgaatat tcctgatggg gcaagtcttc acccaccccg
     8461 gctgttcggc ctgcccggca taactcctca ccgggaccag aagcaaaaaa aataccgacc
     8521 cgggtacagg tcccaggtca gctacaattc acattttgat agtcatttta ccctgaagtt
     8581 cccgaagggt catcgtttac tttatagggc gttgcgccgt agtatgacgg ctcgattcca
     8641 ggttgttagc gcgagattat gcgattttcc agatttatca tcggattgac cagctgtata
     8701 gcgttcagtg ttcaggccgc aaatgttgat gagtacatta ctcaactccc cgctggtgcc
     8761 aaccttgccc tgatggtgca aaaagtcggc gcgtcggccc ccgctattga ttaccacagt
     8821 cagcagatgg cgctgcccgc cagtacgcag aaagtgatta ctgcgctggc ggcgttgatt
     8881 caactcggcc ccgattttcg ttttaccacg acgcttgaaa ccaaaggtaa tgtggaaaac
     8941 ggtgtactta agggtgactt agtagcgcga tttggtgccg atccgacgtt aaaacgtcag
     9001 gatattcgca atatggtcgc gactttgaaa aaatctggcg tcaaccaaat cgatggcaat
     9061 gtgttgatag atacctccat tttcgccagc cacgataaag cccccggctg gccatggaat
     9121 gacatgacac aatgctttag cgctccgcct gccgccgcca tagttgaccg caactgtttc
     9181 tccgtctcgc tctatagtgc cccaaaacct ggtgatatgg cttttatacg cgtggcatct
     9241 tattaccccg ttacgatgtt cagccaggta cgcaccctcc cccgtggttc tgccgaagcg
     9301 caatactgcg agctggatgt ggtgccaggc gacctgaacc gctttacgct gaccggatgc
     9361 ctgccacaac gttctgagcc gctcccgttg gcttttgccg tgcaggatgg tgccagctat
     9421 gccggggcaa ttctgaaaga tgagttaaaa caggcgggta tcacctggag cggaacactg
     9481 ctgcgccaga ctcaagttaa cgaacctgga acggtagttg ccagtaaaca gtcggccccg
     9541 ctgcacgatc tgcttaagat tatgctgaaa aagtcggaca acatgattgc cgatacggtt
     9601 ttccgcatga tcggccatgc gcgcttcaat gtgcctggaa catggcgggc cgggtcggac
     9661 gccgtgcgtc agatcctgcg ccagcaagcc ggtgtcgata ttggtaacac cattattgcc
     9721 gatggttcag ggctttcgcg gcataacctg attgcccccg ccaccatgat gcaggtgctg
     9781 caatacatag cccaacacga caatgaactt aactttatct ctatgctgcc gctggcgggc
     9841 tatgacggct ctttgcagta ccgtgcaggt ctgcatcagg cgggcgtgga tggcaaagtc
     9901 tcagcgaaaa ccggctcgtt gcagggggta tataacctgg cgggattcat taccacggcg
     9961 agcgggcaac gaatggcgtt tgtacaatat ctttctggct atgcagtaga accggcggat
    10021 cagcgtaatc gccgtattcc gttggtacgt tttgaaagcc gtttgtataa agatatttac
    10081 cagaacaatt agtctaaaga gaaaccccgg cgcatggccg gggttttgct gattaacgct
    10141 tgtaaatgaa ctcaacgcct tcttcgtcgt cttcgtccca gtcgtcatcc cagtcttcat
    10201 catcctcttc agcaatctct tcaagctgct gacggtgata atcatcccac atgaattcga
    10261 ctttctctgg ctgtttcgct tcttcagcct gcacgaccgg gttttcaatg ataaaggtca
    10321 tcacatccca gcagagatct ttcacgccca gtccgctcgc cgcagagatc agataatatt
    10381 tatcttccca gcccagcgct tcagcgatcg ctttcgcttt ctcttcggct tctgccttat
    10441 ccagcagatc gatcttgttg aagactaacc aacgcggttt cgccgccaga tcctggctgt
    10501 atttttccag ctcgctgata ataatacgcg cgttttcaac cggatcggtg ccgtcaatcg
    10561 gatcgatatc gatgaggtgc aacagaacgc ggcaacgctc caggtgcttc aggaagcgaa
    10621 tgcccaggcc agcgccttcc gcagcgcctt caatcagtcc tggaatatcg gcaacaacga
    10681 agctcttttc gttgtccatt cgtaccacac caagacttgg caccagagtg gtaaacggat
    10741 aatccgccac tttcggttta gccgccgata ccgcacgaat aaaggtcgat ttacccgcgt
    10801 ttggcatccc caacataccg acgtcagcca gcagcatcag ctccagcagc agctcacgct
    10861 tatcgcccgg cgtaccgtta gttttctgcc gtggtgtacg gttaactgac gatttgaaac
    10921 gggtattgcc cagaccgtgc cagccgccct tagcaaccag cagacgctga ccgtgtttgg
    10981 tcatatcgcc catggtttca ccggtaccct ggtcgataac acgcgtacct accggcactt
    11041 taatcgtcac gtctttaccg cgcttaccgg tacagtcgcg gcttgcgcca ttctgaccac
    11101 gctctgcgcg gaaagatttt tcaaaacgat aatcgataag cgtgttcagg ttctcgtcgg
    11161 cttccatcca tacgtcaccg ccgtcaccgc cgtcgccgcc atccgggccg cctttcggaa
    11221 tatacttttc gcggcggaag ctcacgcaac cattaccgcc atcacctgca acgaccagaa
    11281 tcgatgcttc atcaacaaac ttcattttat tctccgtaaa tcattcgcct gcgcggggtt
    11341 gcgataccac cgttgtatgc ttacgtaatc cgccccaaat acgatgacca atggcggaat
    11401 acatcgcgcc cgcaaccacg acaaacgcac cgagataacc taaaaggttt aacatcggtc
    11461 tggcgaagaa atcgggccag gccagtgata aaagatctga aaaaaacagc gtaaacagtg
    11521 gggtgagcgt gatgatcgcg ctcacctgcg ctgcctgcca gcgagccatc gcttccgcca
    11581 gggcgccata tcctaccaat gtattcagtc cgcaaaaaat taaacatgcg agctgccagt
    11641 ggctaagctg cgctatcact ccaggctttg ccagagggaa gagcgcaatt gtacataaag
    11701 tgtacagtaa aaacaggatc tgcggtgagg ccagccgacg caataaaacc ttttgcgcca
    11761 cgccataact cacccacacc gtcgccgcac cgaccccaaa gattactccc caggtgtaat
    11821 cggtgagctt ggtaaatatc tcgaccagac tggtgttaaa aaacatcacc aggccgctca
    11881 ggagcatcaa tgcccctaca acctgagtgc tgcgcatttt ctctttcagg ataaatacgc
    11941 tggcaaccat catgccaact ggcgagagtt gcccaatcac ctgcgaagcg gtcggactca
    12001 ggtattgcaa ggatgagctg aacaggatga agttcccaaa cagcccggcg gtcgccactg
    12061 ccaacaaaat caaccagcgt ggcttacgaa acacgcgtaa tggcggcaac ctcttcttca
    12121 ccgcaagaat ggcaccaagg ccaatactcg ccatcaagaa acggtaaaac acgattgtcg
    12181 gaggttccat cacctccagc acctgcttca ttgcgattgg caacgccccc cagcaaattg
    12241 ctgtggtgag cgccaaaaga atgccaatgc ctgcctgctg cttcataccg tattccctgc
    12301 aaagcgcatt tgcggattaa cgtcgcacca tgcgcatttt taccggttat cgaatgtaaa
    12361 aagccccgca acgtgttgcg gggctttcat ccgttaccgg gacgcgaaaa acttattcag
    12421 cttcgatgct gataaattta cggtttttcg ggcctttaac ttcgaatttc actttaccgt
    12481 ctgctttagc aaacagagtg tggtcacgac cgcaacctac gttagcgcca gcgtggaatt
    12541 tggtaccacg ttgacgaacg atgatgctac ccgccagaac ggattcgcca ccgaaacgct
    12601 taacgcccag gcgtttagct tctgaatcgc gaccgttacg tgtggagccg ccagcctttt
    12661 tatgtgccat ttgaaatctc tcctcaggtc ttaggcgctg atgccagtaa ttttcacatc
    12721 agtgaaccac tgacgatggc cctgctgctt acgatagtgt ttacgacgac gaaacttaac
    12781 gattttaact ttctcgccac gaccgtgagc aacaacttca gctttgatta cgccgccatc
    12841 aacgaaagga acgccgattt tgacttcttc accgtttgcg atcatcagca cttcagcgaa
    12901 ctcaacagtt tcgccagttg cgatgtccag cttttccagg cgaacggtct gaccttcgct
    12961 tactcggtgt tgtttaccac cactttggaa aaccgcgtac atataaaact ccgcttccgc
    13021 gcacaccttt tcaatgattc agagtgcgct ataaatattc acaatagggc gcgaatatta
    13081 cgcaaaacgc acgcctttga caagtgctac agtcaataca cgaagaaaaa aaacacaact
    13141 tgtacggtaa cgtttatctg tgccattttt tcagtacaat caccctatat tcctaaccat
    13201 aaaccctaag ttgcctttgt tcacagtaag gtaatcgggg cgaaaagccc ggcttttgcg
    13261 atgaatttag aaaaaatcaa tgagttaacc gcgcaagata tggcgggtgt taatgcggca
    13321 atccttgagc agcttaattc cgacgtccaa ctgatcaatc agttaggcta ttacatcgtc
    13381 agcggcggcg gtaaacgtat tcgtccgatg attgctgtac tggctgcacg agctgttggc
    13441 tatgagggaa atgcgcatgt caccattgct gccctgatcg agtttatcca cacggcgact
    13501 ctgctgcacg acgacgttgt ggatgaatca gatatgcgca ggggtaaagc taccgccaac
    13561 gccgcatttg gcaatgccgc cagcgtgctg gtaggcgatt ttatttatac ccgcgcgttc
    13621 cagatgatga ccagcctcgg ctcgctcaaa gtgctggaag tcatgtcaga agccgtaaac
    13681 gtcatcgcag aaggtgaagt tctgcaactg atgaacgtta acgatccgga catcactgaa
    13741 gaaaactaca tgcgcgttat ctatagcaaa accgcgcgtc tgtttgaggc tgccgcgcag
    13801 tgttccggga ttctggctgg ctgtacgccg gaggaggaga aaggcctgca ggattatggg
    13861 cgctatctcg gcactgcttt ccagttgatc gacgatttac tcgattacaa tgccgatggc
    13921 gaacagttag gtaaaaacat cggcgacgat ctgaacgaag gtaaaccgac gctgccgctg
    13981 ctgcatgcaa tgcatcatgg cacaccagaa caggcacaga tgattcgtac cgccatcgaa
    14041 cagggtaacg gtcgccatct tctggaaccg gttctggaag caatgaacgc ttgtggatct
    14101 cttgaatgga cgcgtcagcg tgccgaggaa gaagcagaca aagccatcgc agcgttacag
    14161 gtgctcccgg acaccccgtg gcgagaagca ctcatcggcc tcgcgcacat cgctgttcaa
    14221 cgcgatcgtt aatcccctcc ccctcatccc gcgcagtgcg cgggatgatt ccaataagtt
    14281 ccataaaaac acttattcag cgcaataacg cctgaaaaac cagtgtatgt atctttatat
    14341 attctgaata ttcacactct ttacaggaac tttttagagc aataggccat caggagtata
    14401 gtgatgctcg acagaagaag tgttctgaat gaaagcgact aacttaagga gtgaggaaag
    14461 atggaaagta atttcattga ctggcatccc gctgacatca ttgcgggttt gcgcaagaag
    14521 ggaacgtcaa tggcggcgga atctcgcaga aatggtttga gttcctcaac gctggcgaac
    14581 gcattatcgc gcccctggcc gaaaggagag atgattattg cgaaagccct gggaactgac
    14641 ccctggatta tctggccatc tcgctaccat gatccgcaga cccatgagtt tatcgacaga
    14701 acgcagttga tgcgtagcta cactaaaccg aaaaaatgag tggtctggcg gtagccccgc
    14761 gaacggggct gccagctctc agacaattac tcgcctttca cacgctcaat atttgcacct
    14821 aaagcgcgca gtttgtcttc aatgcgttcg tagccacgat cgatgtgata aatacgatca
    14881 actaccgtcg tcccttccgc aatacagcca gccagcacca ggcttgctga tgcacgtaga
    14941 tcggttgcca taacctgtgc gccagaaagt ttttcaacac cgtgacaaat aacggtattg
    15001 ctttcgattt cggcgtgcgc gcccatacgg ctcagctctg gcacatgcat aaagcggttt
    15061 tcaaagaccg tttcggtgat gaatccggtc ccttctgcca ccaggttcaa cagcgtgaac
    15121 tgggcctgca tatcggtcgg gaatgccgga tgcggcgcgg tacgtacgtt aacagccttc
    15181 ggacgtttgc catgcatatc caggctaatc cagtcttcgc cgacttcgat gtccgctcca
    15241 gcgtcacgca gtttcgccag cacggcgtcc agagtatctg gctgcgcgtt acggcagata
    15301 attttgccgc gagagatcgc cgccgccacc aggaaagtac cggtttcgat acgatccggc
    15361 agcacgcgat agacaccgcc gcctaaacgt tccacacctt caatgacgat acgatcggtg
    15421 ccctgaccgc taattttcgc acccagcgta atcaggaagt tcgcggtatc gacgatttcc
    15481 ggttcacgcg ctgcgttttc aataatcgtg gtgccttcgg ccagggttgc agcacacatg
    15541 atggtcaccg ttgcgccaac gctgacttta tccatcacga tatgtgcgcc tttcaaacga
    15601 ccatcgacgg aagctttaac gtaaccttct tccagtttga tggtcgcgcc taattgttcg
    15661 agaccagaaa tgtgtagatc aaccggacgt gcaccaatcg tacaaccgcc aggcagtgaa
    15721 acttgcccct gaccaaagcg cgctaccagc ggccccagcg cccagataga agcacgcatg
    15781 gttttaacca gatcgtaagg tgcgcagaat acattaacgt cgcgggcatc aatatgcaca
    15841 gaaccattac gttctacttt cgcacccagc tggcttagca gcttcattga tgtatcgacg
    15901 tcttttagtt tcgggacgtt ctggatctct accggttctt ccgccagtag tgcggcaaaa
    15961 aggataggca gagcagcatt tttagcgccg gaaattgtga cttcgccctg gagcttcgtt
    16021 ggcccctgaa cacgaaattt atccatttag tttgttctca gttaacaatt catatccgct
    16081 accggcgaat cgcccatagc tcaaaagccg ttcagtttgc gatcgcgcgc ccactccgca
    16141 ggggtatacg ctttgatcga cacagcatga atgcggttat ccgcaatata ttccatcagc
    16201 gggccataga ccgtctgctg ttttttaacc cgactcatgc cgtcaaacaa ctcacccacg
    16261 gcaataacct gaaagtggct gccatcgccg gaaacgtgga cttcctggag ggagagagcg
    16321 ttcatcaaca cgctctgaat ttcattattt tccatgggat cttcaatcat cagttaataa
    16381 atcagcgaaa catcttagag caaagttgcg ctggcataaa taagcaaaaa gcctcgctga
    16441 taaatcagac aaggctcgac ttgcaggcag gtttgccgga caggcggtta acgccatatc
    16501 cggcctgaaa aaatttaacg aggcagaaca tcagcaggca aattatacaa tttcgcgagg
    16561 gtatacactt tgtcgtttac cccctgaagc gtcacattgt tgccctgctt tttcgccaga
    16621 tcgataagat ggagcagcag tgccagtcca cccgtatcca cgcgggacac acggctaaga
    16681 tcgatgcagg taatcccctt caccgcttcc tcacgcattt cccaaagcgg tagcagaacg
    16741 tcctgatcca gctctccgga tagcgccagc gtgtcacccg tctgcatcca gctcagtgac
    16801 tcgctcatta ttttttctct tccagagtga ttttctgttg agaaatcgat ttcagttgtg
    16861 cagtcaggcc gtcgatacct ttggtacgca gcagcgttcc ccactcgttt tgtttggtgg
    16921 tgatcatact gacgccttca gcaatcatgt cgtaagcctg ccagttgccc gtctgggagt
    16981 ttttacgcca ctggaagtcc agacgcaccg gcgggcggcc atttgggtca ataatggtaa
    17041 cgcgaatagg cacaatggtt ttatcgccca gcggctgttc tggcgcaatc tgataggttt
    17101 gaccgtgata catcgccagc gcctgaccgt aagcctgctt caggtactca cggaaagcgg
    17161 caaagtaagc atcacgttga gctggggtcg cactcttgta atactggccc agcaccagcg
    17221 caccggcgta tttcacctgt acgtatggca acagttcctg atcaacaatg gtacgcagat
    17281 aatccgggtt ggcccgaatt tgcggttgct cattcttcag gcgatcgaac gttttctgcg
    17341 ccgcctcgtc catcagctta tacggattgg tctggtctgc cgcggttgcc gcactcagcg
    17401 gtgcaatcac cagcaaagcg accatcatta aacgtttaaa catgcgtcgg ttctcctgaa
    17461 attatttcgt tgtacccaca ggttcagtgg tttcattatt acctggcgca gcagcaggcg
    17521 catcgccact attcttattg tcatcgcctt tactaccgta aaggaactga ccaatgagat
    17581 cttccagcac catcgcagac ttggtgtcct gaattgtatc gccatccttc aggatagcag
    17641 tccccagttc cgggtcttca aaaccgacgt ttaatgccag atattgttcc cccagcaggc
    17701 cggaagtacg aatgctcagc gaactggtat caggaatgtg gttataacgt tgttcaattt
    17761 ccagcgttac gcgtggcaga taggttttcg ggtccagcgt aatatccgcc acccggccca
    17821 caacaacgcc accaatgctg accggagagc gcgctttcag gccgccaatg ttatcgaacg
    17881 tcgcataaag cgtataggtc ggttcagtac gtatagacgt cacgttcgct gccttcaggc
    17941 aaacaaaaag cgccgcaagc agtgctgcta ataaaaagat acccacccaa atttcatttt
    18001 ttttcgtttg catgaactca attcccaaac atcaatgcgg tcagcacaaa atccagcccc
    18061 agaacagcca gagacgagtg gacaacggtg cgagtggttg cccggctaat cccggcagac
    18121 gtcgggatgg cgtcgtagcc gttaaacaac gaaatccacg tcaccgtgat ggcaaacacc
    18181 acgctcttaa tcagacagtt gaccagatcc atacgccagt cgacggcatt ttgcattgcc
    18241 gaccagaaga acccgctatc aatgcctttc caactgacgc cgaccagcga tccgccccag
    18301 atccccacgg cgacgaagat aaccgtcaac agtggtaatg aaataacccc agcccagaaa
    18361 cggggagaaa taacccgacg cagcggatcc accgccatca tctccatact ggagagttgc
    18421 tctgtagcgc gcatcaggcc gatttctgcg gttagcgccg aaccggcacg cccggcaaac
    18481 aacagcgcgg caaccaccgg ccccagttca cgcagtagcg ataacgccac cagcataccc
    18541 agactggttt ccgcactata agtggtcaga accagataac cttgcagccc caacaccatt
    18601 ccgatgaaca cgccagaaac tacaataatc agcatcgaca ggacgccgac attatagagc
    18661 tggcgcacca gcagcggcgc atgtttgcga aattccggtt tgccgaccag cgcattgaat
    18721 aacattaacc cggcccgccc gaacgttctc agggttttaa tccctttatg tccgagcgac
    18781 gccagcgcat ttaacagcat gagtggctta actccctggt aaaagatcag cgtgataatc
    18841 gccggcagga tagcggaacg aaacaggccc gtcagctatc ccgtccagaa actgacgtac
    18901 gcgcggatca ggattcgcct gcaacgcctg ggcactgcca tgagcgacaa tttttttgtc
    18961 cgccaggatc caggcgtgat cggcaatact taacacttcc ggcacatcgt gagaaaccac
    19021 cacacaagtc acacccagcg cgctgttcag ctcagaaatc agcttcacca gtacgcccat
    19081 ggtgatggga tcttgcccaa caaagggctc atcaaacatg atgagatccg gctccagcgc
    19141 aatcgcacgt gccagcgctg cacgccgcgc catcccaccg gaaagttcag aaggcataag
    19201 tttagccgct ccacgcagcc ccactgcctc cagcttcatc atcaccgtac tatgcaacaa
    19261 tggcgcggga agttgggtat gttcccgcag tggataggcg acgttgtcaa atacgttcat
    19321 atcagtgaac aacgccccgg actgaaataa catgctcatc cgtttgcgca ctgtatacag
    19381 gcgcgaacga gacatcgccg gaatattctc accatcgaaa aggatctcac catgatctgg
    19441 tgcgatttgc ccgccaatca gacggagtag cgtcgtttta ccgatgcccg atggccccat
    19501 gatcgccgtg atcttccctc gcggcacggt cagggaaata ttatcgaaga tgcagcgatt
    19561 gccacgcgta aaactgacat cgcgcatatc gactaaattc gccacagact gctccataat
    19621 tcacccttcg tcttgcgttg attttctaag catggcgctc aatttaacct tgaacccaac
    19681 atatttacag aatattaccc gccgtggtta gcgaaagctg gcatttgttt tactttttag
    19741 ccgcataaag tcaaaattaa gaatccgtta cggctttctg aaaatcttca gcggaccggc
    19801 gagtatacct gaagaaagga cgttagatgc ttttagctac ggcactgtta attgttggtt
    19861 tacttttggt cgtttacagt gccgaccgcc tggtttttgc cgcgtctatt ctttgccgaa
    19921 cctttggcat cccgccgcta atcatcggca tgacggtggt cagtattggt acatcgttac
    19981 cagaaatcat cgtctcgctt gccgcgtctc tgcacgaaca acgcgattta gccgttggta
    20041 cagccctcgg ctcaaacatt atcaatatat tgctgatcct cggtctggct gcgctggttc
    20101 gtccttttac cgtccattcc gatgttctac gccgtgaatt acccttaatg ttgttggtca
    20161 gcgtggtggc cggttccgta ctctatgacg gacaacttag tcgcagcgat ggtatctttc
    20221 tcctctttct ggctgtgcta tggctgctgt tcattgttaa acttgcacgt caggctgaac
    20281 gtcaggggac tgacagcctg accagagagc agcttgcaga gctgccgcgt gacggcggat
    20341 tgcccgtcgc gtttttatgg ctcggcattg cgcttatcat catgccagtg gccacgcgga
    20401 tggtggttga taacgccacg gtgctggcga attactttgc catcagcgag ttgacgatgg
    20461 gtctgacggc aattgctatc ggaaccagcc tgccggaact ggcaaccgca atagcggggg
    20521 ttcgcaaagg tgaaaacgac attgcggtcg gaaatattat tggcgcaaac atctttaata
    20581 ttgtcatcgt gttgggttta cccgcgctga taacgccagg agagattgat ccactggcgt
    20641 acagtcgtga ctacagcgtg atgttgctgg tgagcattat ttttgcgttg ctgtgctggc
    20701 ggcgctcccc gcaatcgggc cgtggtgtag gggtattatt aactggcgga tttatcgtat
    20761 ggctggcgat gttgtactgg ttatcgccaa tactcgttga ataactggaa acgcattatg
    20821 tcgcacgtag agttacaacc gggttttgac tttcagcaag caggtaaaga agtcctggcg
    20881 attgaacgtg aatgcctggc ggagcttgat caatacatca atcagaattt cacgcttgcc
    20941 tgtgaaaaga tgttctggtg taaagggaaa gttgtcgtca tggggatggg gaaatcgggg
    21001 cacatcgggc gcaaaatggc ggcaacgttt gccagcaccg gtacaccttc atttttcgtc
    21061 catcctggtg aagccgcgca tggtgattta ggcatggtca ccccacagga tgtggtgatt
    21121 gctatctcta actcaggtga atccagcgaa atcacggcct taattccagt gcttaagcgt
    21181 cttcacgtac cgttaatctg catcaccggt cgcccggaga gcagcatggc gcgcgccgca
    21241 gatgtgcatc tgtgtgttaa agtagcgaaa gaagcctgtc cgttagggct ggcaccgacc
    21301 agcagcacca ccgccacgct ggttatgggc gatgccctcg ctgtcgcgct gttaaaagca
    21361 cgcggcttta ctgctgaaga ttttgcgctc tcacacccag gcggcgcact gggtcgtaaa
    21421 cttctgctgc gcgtaaacga tattatgcat acgggcgatg agatcccgca tgttaagaaa
    21481 acggccagtc tgcgtgacgc gttgctggaa gttacccgca aaaatcttgg tatgactgtc
    21541 atttgcgatg acaatatgat gattgaaggc atctttaccg acggtgattt acgccgtgtc
    21601 ttcgatatgg gcgtggatgt tcgtcagtta agtattgccg atgtgatgac gccgggggga
    21661 atacgtgtgc gccctggcat tctggccgtt gaggcactga acttaatgca gtcccgccat
    21721 atcacctccg tgatggttgc cgatggcgac catttactcg gtgtgttaca tatgcatgat
    21781 ttactgcgtg caggcgtagt gtaaagattc aaggataaac aacaatgagc aaagcaggtg
    21841 cgtcgcttgc gacctgttac ggccctgtca gcgccgacgt tatggcaaaa gcagagaata
    21901 ttcgtctgct gatcctcgat gtcgatggcg tactgtcaga tggcctgatt tatatgggca
    21961 ataatggcga agagctgaaa gcgttcaatg ttcgtgacgg ttatggcatt cgttgtgcgc
    22021 tcacctctga tattgaagtc gctatcatta ccgggcgaaa ggctaaactg gtagaagatc
    22081 gttgtgccac attggggatc actcacttgt atcaggggca gtcaaacaaa ctgatcgcct
    22141 ttagcgatct gctggaaaaa ctggcgattg ccccggaaaa tgtggcttat gtcggcgatg
    22201 atctcatcga ctggccggta atggaaaaag tggggttaag cgtcgccgtg gccgatgcgc
    22261 atccactgtt gatcccgcgc gccgattacg tgacgcgcat tgctggcggt cgtggcgcag
    22321 tgcgagaagt ttgcgactta ttactcctgg cgcagggcaa actggatgaa gccaaagggc
    22381 aatcgatatg agtaaagcca gacgttgggt tatcattgtg ctatcactgg cggttctggt
    22441 gatgatcggc attaatatgg ccgaaaaaga cgataccgcc caggtggtcg tcaacaacaa
    22501 tgatcccacc tataaaagcg agcatacgga cacgctcgtc tataacccag aaggggcact
    22561 aagctatcga ttgattgctc aacatgttga atattattcc gatcaggccg tttcgtggtt
    22621 tacgcagccg gtacttacca cgtttgataa ggataaaatc ccgacatggt cagtaaaagc
    22681 agataaagcc aagctgacca atgaccggat gctctattta tatggacacg ttgaagtcaa
    22741 cgcacttgtg ccggactctc aacttcgcag aatcacgacg gataacgcgc agatcaatct
    22801 ggtgacgcag gatgttacct ctgaagacct cgtcacgtta tacggaacaa catttaactc
    22861 cagcggtctg aaaatgcgcg gcaacttacg cagcaagaac gccgagctga ttgaaaaggt
    22921 tagaacatcc tatgaaattc aaaacaaaca aactcagcct taatcttgtg cttgccagct
    22981 cacttctggc cgccagcatt ccggcatttg ctgtaaccgg agacactgat cagccgatcc
    23041 acattgaatc ggaccagcaa tctcttgata tgcaaggcaa cgtggttacc tttaccggta
    23101 atgtcatcgt cacccagggc accatcaaaa ttaatgccga caaagtggtc gttacccgtc
    23161 cgggcggcga acaaggtaaa gaagtgattg acggctacgg taaaccggca acgttctacc
    23221 agatgcagga caatggtaaa cccgttgaag gtcacgcttc ccagatgcac tacgaactgg
    23281 caaaagattt tgtcgttctg acgggtaatg cttatctgca gcaggtcgat agcaacatta
    23341 agggcgataa gatcacttac ctggtgaaag agcagaaaat gcaggctttc agcgacaaag
    23401 gtaagcgcgt aaccaccgtt ctggtgccgt cgcagctgca ggacaaaaac aacaaaggcc
    23461 agaccccggc acagaagaag ggtaattaat tcgttatggc aacattaact gcaaagaacc
    23521 ttgcaaaagc ctataaaggc cgtcgcgtgg tagaagacgt cagcctgacc gtcaactccg
    23581 gggaaattgt cggtctgctg gggccaaacg gtgccggtaa gaccaccact ttctacatgg
    23641 ttgtaggcat tgtgccgcgc gatgcgggca acatcattat tgatgatgac gatatcagtc
    23701 tgctgcctct gcatgcacgc gcgcgccgcg gtatcggcta tctgccacag gaagcctcca
    23761 ttttccgtcg cctcagcgtt tacgataacc tgatggcggt actgcaaatt cgtgacgact
    23821 tgtctgctga acaacgtgaa gaccgcgcga acgagctgat ggaagagttt cacattgagc
    23881 acctgcgtga cagcatgggg cagtcactct ccgggggtga acgtcgccgt gtagaaattg
    23941 cccgcgcact ggctgcgaat ccgaaattta ttctgctcga cgaaccgttt gccggggttg
    24001 acccgatctc ggttatcgac attaaacgca tcattgaaca cctgcgcgac agcggcctgg
    24061 gcgtactgat cactgaccac aacgtgcgtg aaacactggc ggtttgtgaa cgcgcttata
    24121 tcgtcagtca ggggcatttg atcgcccacg gcacgcctac agaaatctta caagacgaac
    24181 acgttaagcg tgtatacctt ggggaagact tcagactctg atagggtaga agtttgcgac
    24241 gttttagcag gagagtacga ttctgaacat gaagcaaggt ttgcaactca ggcttagcca
    24301 acaactggcg atgacgccac agctccaaca ggcaattcgt ctgttgcagt tgtcgacgct
    24361 ggaacttcag caggagctac agcaggcgct ggagagtaat ccgctgcttg agcaaatcga
    24421 cactcatgaa gaaatcgaca cccgcgaaac gcaagacagt gaaacgctgg acaccgccga
    24481 cgcgctcgaa caaaaagaga tgccggaaga gctgccgctc gatgccagtt gggacaccat
    24541 ttacaccgct ggtacaccat ccggcaccag cggtgactac attgacgacg agctgccggt
    24601 ctatcagggc gaaacgacgc agaccttgca ggattacctg atgtggcagg tcgagctgac
    24661 accgttttcc gacactgacc gcgctattgc tacctctatc gtcgatgccg ttgatgacac
    24721 cggttatctg actgtcccgc tggaagatat tctcgaaagt atgggcgatg aagagattga
    24781 catcgacgaa gttgaagccg tccttaagcg gatccaacgg tttgatccag tcggtgtggc
    24841 ggcaaaagat ctgcgtgact gtctgctgat ccaactctcc caattcgata aaaccacgcc
    24901 gtggctggaa gaggccagac tgattattag cgatcatctc gatctgttag ccaatcacga
    24961 cttccgcact ttaatgcgcg tcacgcgtct gaaagaagat gtgctgaaag aagccgtcaa
    25021 tctgatccag tcgctcgatc cgcgccccgg gcagtcgatc cagactggcg aacctgagta
    25081 tgtcattcca gatgtgctgg tgcgtaagca taacggtcac tggacggtag aactcaacag
    25141 tgacagcatt ccgcgtctgc aaatcaacca gcactacgcc tcgatgtgca ataacgcgcg
    25201 caacgatggt gacagccagt ttatccgcag caatctgcag gatgccaaat ggttgatcaa
    25261 gagtctggaa agccgtaacg atacgctact gcgcgtgagt cgctgtatcg ttgaacagca
    25321 gcaggccttc tttgagcaag gtgaagaata tatgaaaccg atggtactgg ccgatatcgc
    25381 ccaggctgtc gaaatgcatg aatcgacgat atctcgcgtg accacgcaaa aatacctgca
    25441 tagtccacga ggcatttttg aactgaagta tttcttttcc agtcacgtca ataccgaggg
    25501 cggcggcgaa gcttcctcca cggcgattcg tgcgctggtg aagaaattaa tcgcggcgga
    25561 aaacccagcg aaaccgttga gcgacagcaa gttaacctct ttgctgtcgg aacaaggtat
    25621 catggtggca cgccgcactg ttgcgaagta ccgagagtct ttatccattc cgccgtcaaa
    25681 ccagcgtaaa cagctcgttt gacccaaccg ataaggaaga cactatgcag ctcaacatta
    25741 ccggaaataa cgtcgagatc accgaggcac tgcgcgaatt tgttacagcc aaatttgcca
    25801 aacttgagca atattttgac cgaatcaacc aggtctatgt tgttctgaaa gtggagaaag
    25861 tcacccacac ctcagatgca acactgcatg taaacggtgg tgaaattcat gccagcgcgg
    25921 aaggtcagga catgtacgct gccattgatg gcttaattga taagctggca cgccagttga
    25981 ccaaacataa agataaactg aaacaacact aattgtccgg gcaattagca tgtgcatggc
    26041 ggcctgttgt gcggcacaac gggccatttg tacggttaat gctccgagcc tgttccactg
    26101 tttgagtggg caggttctta ggtgaaatta tgacaaataa tgatacaact ctacagctta
    26161 gcagtgttct taacagggaa tgtacgcgaa gccgcgtcca ctgtcagagc aaaaaacgcg
    26221 ccctggaaat catcagcgag ctggcggcga aacaacttag cctgccgcct caggtggttt
    26281 ttgaagctat cctgacgcgg gaaaaaatgg gcagtaccgg tatcggcaat ggtattgcca
    26341 ttccgcatgg caaactggaa gaagatactc tgcgcgccgt tggcgttttc gttcagctcg
    26401 aaacgcctat agctttcgat gccatcgaca accaaccggt ggatttactt tttgccctgc
    26461 tggtgccggc agaccaaact aaaacgcacc tgcatactct gtcactggtg gcgaaacgtc
    26521 tggcggacaa aaccatctgc cgccgtttac gcgcagccca gagcgatgaa gagctgtatc
    26581 aaatcattac ggataccgaa ggtactccgg atgaagcgta gttattcggt aatgtctctt
    26641 ttagacgttg tgaggagaaa cagtacatgg tactgatgat cgtcagcgga cgttcaggtt
    26701 caggtaaatc tgtcgccctg cgtgcgctgg aagatatggg tttttactgc gtggataacc
    26761 ttcccgtagt gttgttaccc gatctggctc gaactctggc cgatcgcgag atttctgccg
    26821 ccgtcagcat tgatgttcgt aatatgccgg agtcaccaga aatattcgaa caggcgatga
    26881 gtaacctgcc tgacgctttc tcaccgcaac tactgttcct ggatgccgac cgtaatacct
    26941 taattcgtcg ttacagtgac acgcgccgac tgcatccgct ttccagcaaa aacctgtcgc
    27001 tggaaagtgc tatcgacaaa gaaagcgatt tgctggagcc tctgcgttcg cgagcggatc
    27061 tgattgtcga tacctcagaa atgtccgttc acgagctggc tgaaatgctg cgtacccgtc
    27121 tgctgggtaa acgtgaacgc gaactgacca tggtctttga gtctttcggc ttcaaacacg
    27181 gtatccctat cgatgcagat tacgtctttg acgtgcgctt cttgccgaac ccgcactggg
    27241 atccgaaact gcgtccaatg acaggtctgg ataagcctgt cgccgcgttc ctcgaccgcc
    27301 acacagaagt acacaatttt atctaccaga cgcgaagcta tcttgagcta tggttaccta
    27361 tgctggaaac caacaaccgt agctacctga cggtcgccat tggttgtacc ggcgggaagc
    27421 accgttcggt gtatattgca gagcaactgg ctgactactt ccgctcgcgc ggtaaaaacg
    27481 tccagtcacg ccatcgtacg ctggaaaaac gtaaaccatg accgtcaagc aaactgttga
    27541 aatcacaaac aagctgggca tgcatgcccg gcctgcaatg aagctgtttg aattaatgca
    27601 aggttttgac gctgaagtgc tgttacgtaa tgacgaaggc accgaggctg aagccaacag
    27661 cgttattgcg ctgctgatgt tggattctgc caaaggacgg cagattgaag ttgaagcgac
    27721 cggtccacag gaagaggaag cactggccgc cgttatcgcc ctctttaatt ctggttttga
    27781 tgaagattaa tcttcatcac tttgacatac aactcccttc aaactccccc cgacaataag
    27841 aaaatcacgt actgaaatcg ttctcaatca acgtcatttg tacattttgt gcgcttttta
    27901 ctttcagaag aaccttaaga aaaccttaag aggcattgtt taggttttgt ttaagttaat
    27961 cgaccatact ggagatcgtc agaaaatatt tccaggagat ggcatgattc gcttatctga
    28021 acaaagtccc ctgggcaccg gaagacatcg gaaatgttat gcgcatccgg aagatgccca
    28081 acgctgtatc aagattgtct accatcgtgg cgatggtggc gataaagaga tccgccgtga
    28141 gttaaagtac tacgcgcatc ttggtcgccg gttaaaagac tggagtggaa taccgcgcta
    28201 tcacggtacc gttgagacgg attgcggaac cggttatgtc tacgatgtga ttgcagattt
    28261 tgacggcaag ccctccatta cgctgaccga atttgctgaa caatgccgtt acgaagaaga
    28321 cattgcacaa ctgcgccagc tactgaaaca gctgaagcgt tatttacagg ataaccgtat
    28381 cgtgacgatg tcgctgaagc cgcagaatat cctttgtcat cgcatcagtg agtcagaggt
    28441 tatccccgtc gtctgcgaca acattggtga aagcacattg attccactgg cgacctggtc
    28501 gaagtggtgt tgtctgcgta agcaagagcg gttgtggaaa cgatttatcg cccaaccggc
    28561 gctggctatt gccctgcaga aagatctgca acctcgtgag agcaaaacgc tggccctcac
    28621 ttcgcgcgaa gcttaatcaa gctggtgttg ttgcataaac ggctcaccgc ctaactgata
    28681 catctgccgt aaaatccacg cctgacggct acgcacgtag cccgatggcg cggagacttt
    28741 gaaacgaagt ggattaggta atacagctgc cagtaatgca gcttccgacc gggtaagctt
    28801 gctcgcgggt ttgtggaaat aacgttgtgc cgcagcttcg acgccaaaca caccgtcgcc
    28861 aaattcggcg atattcaggt aaaccgtcag gatacgcttt ttgctccaga ccgtttctat
    28921 ccccagcgtt aatccggctt ccagcccttt tcgaacccag ctgcgcccat cccataaaaa
    28981 gagatttttg gctgtctgct gagaaatcgt tgacgcaccg cgaatacggt tttcattgcg
    29041 ttcgttgtgc gccagggctt gctcaatgga agcgacatca aagccccagt gctcaggaaa
    29101 tttctgatct tctgcggcaa tcaccgccag tcccatccac ggcgagattt gatccatact
    29161 gacccagtca gaatgtgcca cgtaacgaaa attgccatgc agccaggcgc tgacttgccg
    29221 ctcaaccatt accgctgaga agggaacagg cgcaacgcta aacaacgcga tgcccccgcc
    29281 ccagaaaacg gcgaggacaa ccattaaccg cagaagaaaa cggcgaacga aactaaacac
    29341 cgttaagcgg cttttactca ttccgccagg accagcacgc gggaaaccag cttatcaatg
    29401 ccgctcgccg cttctgcaat gttctgcgcc agcatatatg caggggtggt gacaatctta
    29461 ttgtcttcat caaccacgat atcatcgaca ggacacggca catgctccgc gcccatctct
    29521 tccagcactt ctgcggtatc aatatcagta ccgatggtca aacgcagcgg gaaatcgaaa
    29581 attttcggca gcatcgccgg ggcaatacac ataaaaccaa gcggttttcc ggcctgatgc
    29641 atcgcttgtg ccagcgcctt taattcacgg tcaacggtgc attcactccc cagacgggca
    29701 aagttgctta aattcttcgc cgcaccaaac ccccccggca caatcaacgc atccagctca
    29761 gcggcatcgg cctgggccag agggcggatc tcaccacgcg ttattcgtgc cgcttcaatc
    29821 agcacattgc gcgtttccgg catcgcttcg ccagttaaat gattgattac atcaacctgc
    29881 tgcttatccg gtgcaaagca gaccgcctgc gcaccgctgc gtgaaatagc taacaacgtc
    29941 aacaccgctt catgaatttc agaaccgtca tagacgccgc atccgctcag aattacgcca
    30001 attttcttca ttgtgatcat cctttcgcaa ttgactgaaa cacattaatt tttttaataa
    30061 aaatgctacg catcacacat ttaactgatt catgtaacaa atcatttaag ttttgctatc
    30121 ttaactgcgt gcggcctgaa aaacagtgct gtgcccttgt aactcatcat aataatttac
    30181 ggcgcagcca agatttccct ggtgttggcg cagtattcgc gcaccccggt ctagccgggg
    30241 tcatttttta gtagcttttg ccacccacgc tttcagtact tctacgtcgt gacgccactc
    30301 ttctttcatc tcttcaatcc attcaccgac gttatcttcc caggcgggaa ggtcaggaga
    30361 ctgaatttgc tgacccaact gttgcagatg gcgtaacccc actgaccccg ccgcaccttt
    30421 aattttatgt ccttcctcaa caatgccttt tttatcctgc gccgtcagat tcgactccag
    30481 cacgctaaca tatcccggca tcatcttctc aaataccgct aacccgtcgg tgatcagctt
    30541 cggtcctaca agttcgagat actgttccag catgggaata tcgagcaatg cttctgattt
    30601 actgttctct tctgtcgtca ccgtactctc ctcatcatcc tgggtatccc agaatttctt
    30661 gatcatcgcg gttagcgccg gaacagaaag cggcttactc agcacatcat ccattccagc
    30721 attgaggtac tcttgtttgt ctttcagcac gttagcggtt aaggccacca gcggtggtaa
    30781 atcctcgcgc ggataacgct tcgtcagttc acgagagata tccagcccgg tcatatctgg
    30841 caactgaata tccagcaata ccaggtcgta ttcgcccggt ttaaacatct ccagcgccgc
    30901 cttgccggtc atggcgacat caacgctgtt acctaatttt tccagcacag aacgcgcgac
    30961 aatcacgttc agttcaatgt cttccaccag cagtacattc agcgccggta aaggcatatc
    31021 gtcttcatca aacgcatcat cgacctcttc tgccaccgac ggtgcgtgga tcgtcaacgt
    31081 aaaggttgaa cctttgccct gttcgctggt aaccgtaata tcgccgccca tatttttcgc
    31141 cagacgacga gaaacggcca gaccaatacc ggtgccggtg gcaggtttac cgccatgact
    31201 gtctttcacc tggtaataca tggcgaaaat tttatccagc tcatcctgcg gaatgccaat
    31261 gccggaatct tccacttcaa aatgcagcat atcgccttca tcgtagcgca cgcgcacggt
    31321 aacctggcct tgctgggtga atttgacggc gttactgatg aggttccaca ggatctgccg
    31381 taaacgcgtc ccgtcggtaa tgacctgatg cggtaatggc agcgtaggct ccaggttaaa
    31441 gcgcaatcct ttttgttgcg cct
//
LOCUS       DAMZFT010000049        31434 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.049, whole genome shotgun sequence.
ACCESSION   DAMZFT010000049 DAMZFT010000000
VERSION     DAMZFT010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 31434)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31434)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31434
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.049"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..376
                     /locus_tag="PN977_002324"
     CDS             <1..376
                     /locus_tag="PN977_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000220508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6042352.1"
                     /translation="NAMFVMYGCRTSYASDDSGQGFASKLSLLDNFKDVNVWGQTEST
                     YPSYFPDIRTTSIMRSINIGWSFSPTYMVASSEGQGWDALFPDDKNPLKSLPMQCYNN
                     GKLILTCDQSSFNDHRKNKSND"
     gene            369..908
                     /locus_tag="PN977_002325"
     CDS             369..908
                     /locus_tag="PN977_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000623467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6042353.1"
                     /translation="MISIILRSLILLFMIIVYPDYNKNLPAFEVYGENDILKDMVINM
                     KTYSNHSKKNSSPTTIVQTILFKGNIQQVFESNDMGAASFFFSYNNKVETLVTDAFFL
                     NRKLDKLHDANIFEVIKHGENIVIRYYKCKIEYMKKEKSIEIEISTNKNSYFSKNGIK
                     QKDTNKYNTYFFNYFEAQK"
     gene            complement(920..1315)
                     /locus_tag="PN977_002326"
     CDS             complement(920..1315)
                     /locus_tag="PN977_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyltransferase/cytidyltransferase family
                     protein"
                     /protein_id="HDI6042354.1"
                     /translation="MKTVITFGTFDVFHVGHLRLLQRARTLGERLLVGVSSDALNIAK
                     KGRAPVYPQDDRMAIIAGLACVDGVFLEESLEQKAEYLRGYSADILVMGDDWAGKFDS
                     FAYICEVVYFPRTPSVSTTGIIEVIRGKA"
     gene            complement(1318..1752)
                     /gene="fliJ"
                     /locus_tag="PN977_002327"
     CDS             complement(1318..1752)
                     /gene="fliJ"
                     /locus_tag="PN977_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001248915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export protein FliJ"
                     /protein_id="HDI6042355.1"
                     /translation="MRQIIDTLAQLQRLRDKSVKDKTVELAKQKQICAGYDNNIKALG
                     YLVEKTSAGAAASVESLKNVSDYKGTLRKVIAWQEQEKTLANIKATRMQKNLTAAACE
                     EKVVALTLDDKRREQQESATAKAQKAVDDIAVQCWLRHKLAE"
     gene            complement(1755..3092)
                     /gene="fliI"
                     /locus_tag="PN977_002328"
     CDS             complement(1755..3092)
                     /gene="fliI"
                     /locus_tag="PN977_002328"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001279390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein export ATPase FliI"
                     /protein_id="HDI6042356.1"
                     /translation="MSDFSAFDNALRSLESIPLARVAGRLVRLNGILLESVGCPLMTG
                     QLCRIESANHTLIDAQAVGFNRDITYLMPFKQPVGLMAGARVFPEEKAHDILIGESWL
                     GRVVNGLGEPLDGKGRLNGNDLLPPLPPSVNPLTRRSVDEPLDVGVKAINGLLTIGKG
                     QRVGLMAGSGVGKSVLLGMITRQTKADIVVVGLIGERGREVKEFIDHSLGADGLAKSI
                     VVVAPADESPLMRLKATELCHSVAAWFRDRGHHVLLLVDSLTRYAMAQREIALSLGEP
                     PATKGYPPSAFGMIPKLVESAGNSESAGSMTAIYTVLAEGDDQQDPIVDCARAVLDGH
                     IVLTRKLAEAGHYPAIDIGQSISRCMNQVTSLEHQQSARALKQNYAAYMEIKPLIPLG
                     GYVAGADASVDKAVKMFPAIERFLRQEMREPASLELVQSRLQILFPGVKKAEQ"
     gene            complement(3085..3795)
                     /locus_tag="PN977_002329"
     CDS             complement(3085..3795)
                     /locus_tag="PN977_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001521882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein H"
                     /protein_id="HDI6042357.1"
                     /translation="MTIETLRGRYRLHRFPPRQRAVTGEPLTASISAADYQRQLMDGF
                     QEGLQKGFAQGMTEGQEQGFSEGHQQGFAEGRRQGYTEGSLAGQQEGRKQFVDAAQPL
                     EAISGKVNDFLAHIERKQREDLLQLVEKVTRQVIRCELALQPTQLLALVEEALAAFPA
                     MPETLQVMLSTEEFNRLRDAVPEKVSEWGLTPSPDLPPGECRVITDKSELDIGCEHRL
                     EQCMTALQETLTPESQGE"
     gene            complement(3798..4808)
                     /locus_tag="PN977_002330"
     CDS             complement(3798..4808)
                     /locus_tag="PN977_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001293121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HDI6042358.1"
                     /translation="MSELTTNKSNSYLEQAAILLLCLGEEAAATVMQKLSREEVVRLS
                     ENMARLSGVKTSMARKVINNFFDEFREQSGINGASRSMLQGILNKALGTEIASSVING
                     IYGDEIRSRMARLQWVEPRQLAMLISEEHLQLQAVFLAFLTPEISAAVLSYLNESVQN
                     EILYRVAKLNDVNRDVVDELDRLIERGLSVLSEHGSKVKGIKQAADIVNRFQGNQQVI
                     LDQMRERDEDVLEQLQDEMYDFFILSRQNEEVRRRLLDEVPMEDWAVALKGTEALLRR
                     SIYAVMPKRQAQQLEAITARLGPVPVSRIEQIRREIMGIARELEEAGEIQLQLFAEQT
                     AE"
     gene            complement(4786..6432)
                     /gene="fliF"
                     /locus_tag="PN977_002331"
     CDS             complement(4786..6432)
                     /gene="fliF"
                     /locus_tag="PN977_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001643010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar M-ring protein FliF"
                     /protein_id="HDI6042359.1"
                     /translation="MNAQIKKLTQAFPAFRLRLADNKRWALMAGVGLAVAATAIIVSV
                     LWTGNRGYVSLYGRQENLPVSQIVTVLDGEKLSYRIDPQSGQILVPEDELSKTRMTLA
                     AKGVQAILPSGYELMDKDEVLGSSQFVQNVRYKRSLEGELAQSIMSLDAVESARVHLA
                     LNEESSFVVSDEPQNSASVVVRLHYGAKLNMDQVNAIVHLVSGSIPGLHASKVSVVDQ
                     AGNLLTDGIGAGEAVSAATRKRDQILKDIQDKTRASVANVLDSLVGSGNYRVSVMPDL
                     DLSNIDETQEHYGDAPKINREENVLDSDTNQVAMGVPGSLSNRPPIAANQMTNGTEEN
                     RSPEALSKHSESKRDYSYDRSVQHIQHPGFAVKRLNVAVVLNQNAPALKNWKPEQTTQ
                     LTALLNNAAGIDVQRGDNLTLSLLNFVPQAVPVEPIIPLWKDDSVLAWVRLIGCGLLA
                     LLLLFFVVRPVMKRLTAVRAPVITPEPEAVSEPWIAMPEEERKNVDLPSLPGDDSLPS
                     QSSGLEVKLEFLQKLAMSDTDRVAEVLRQWITSNERIDNK"
     gene            complement(6437..6778)
                     /locus_tag="PN977_002332"
     CDS             complement(6437..6778)
                     /locus_tag="PN977_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HDI6042360.1"
                     /translation="MSITTINSTMQAQIMQDVQRMQANAQAPVLPAMTFSSTDPDVSF
                     NRIMSGALGHVDQFQQVAEQQQTAVDTGKSDDLAGAMIASQQASLSFSALVQVRNKIA
                     TGFNDLMSMSI"
     gene            complement(6793..7779)
                     /locus_tag="PN977_002333"
     CDS             complement(6793..7779)
                     /locus_tag="PN977_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001292201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent Fis family transcriptional
                     regulator"
                     /protein_id="HDI6042361.1"
                     /translation="MSELIATAASSINAFTLAKRVAAFNVPVLIQGETGAGKECVAKY
                     IHTVAFGENDNAPYIGVNCAAIPENMLESTLFGYDKGAFTGAIASVPGKMELANNGSL
                     LLDEIGEMPLALQAKILRVLQEQQVERLGSNRQIKLNFRLIACTNKNLEQEVAAGRFR
                     EDLYYRLAVIPITMPPLRERLNDIIPLAESFIKKYSTVLVKNITLSESTRRALLNYRW
                     PGNVRQLENAIQRGMILNRDGVIYPDALGLPDTDIADHSELQWPVQPAVHIAETGDLG
                     QHGRSAQYQYIADLMRKYQGNRSKIADLLGITPRALRYRLASMRKHGIEVFS"
     gene            8152..9003
                     /locus_tag="PN977_002334"
     CDS             8152..9003
                     /locus_tag="PN977_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000922508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliM/FliN family flagellar motor switch protein"
                     /protein_id="HDI6042362.1"
                     /translation="MLKYSKTPGIFKLEGNRLGRPYHRLPTLFTGNFDVIDSHLGSYF
                     LKKHRSNITLKKIGCEMDIINKNAELMVSQVGHLAFDIDRSLLLMLLGNFYGLESSLE
                     EAKAHNGLPTKTETRLKNRLALDICTLIFNLQTSGIALKLKLDSSTVITHWAYQLTFT
                     LAGDEESSFRILLDDAHTDFILNLIRHSEHSKPQQVAKSVNKPALIKEIIRSLPLTLN
                     VKIAELSMNVADLAQIKAGDILPISLGETFPVAIGQSELFSALIVEDKDKLFLSELAG
                     KNENSHE"
     gene            8996..9367
                     /locus_tag="PN977_002335"
     CDS             8996..9367
                     /locus_tag="PN977_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliM/FliN family flagellar motor switch protein"
                     /protein_id="HDI6042363.1"
                     /translation="MSKQDDILAGDFGLADEVAPVTKSADAETLVTRLEDRFSDSMTL
                     LKRIPVTLTLEVSSVEIMLADLLNIDDDTVIELDKLAGEPLDIKVNNILLGKAEVVVV
                     NEKYGLRVLEFNTRDINDLAP"
     gene            9364..10116
                     /gene="fliP"
                     /locus_tag="PN977_002336"
     CDS             9364..10116
                     /gene="fliP"
                     /locus_tag="PN977_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538309.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HDI6042364.1"
                     /translation="MKRRTQLALGLGLLALAPLALAQGGDIALLNVVTHGNTQEYSVK
                     IQVLILMTLVGLLPTMVLMMTCFTRFIIVLSLLRQALGLQQTPPNRILIGIALSLTML
                     VMRPVWLNIYDHAVVPFENDQITLTDALSTAATPLKRFMLAQTDKKAMAQIMTIGGAK
                     GNAADQDLTIVVPAYVLSELKTAFQIGFMIYIPFLVIDLIVASVLMAMGMMMLSPLIV
                     SLPFKLMLFVLIDGWSLTIGTLTTSIRGLGLG"
     gene            10119..10391
                     /gene="fliQ"
                     /locus_tag="PN977_002337"
     CDS             10119..10391
                     /gene="fliQ"
                     /locus_tag="PN977_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001341234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FliQ"
                     /protein_id="HDI6042365.1"
                     /translation="MLTVDVAADIVASGIKVVILLVSVLVVPSLLVGLLVSVFQAVTQ
                     INEQTLSFLPRLIVTLVVLGVCGKWMIIQLHDLCIHLFSQAALLVH"
     gene            10393..11175
                     /gene="fliR"
                     /locus_tag="PN977_002338"
     CDS             10393..11175
                     /gene="fliR"
                     /locus_tag="PN977_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001260509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliR"
                     /protein_id="HDI6042366.1"
                     /translation="MRTSDVTQLTDLALGLWFPFVRIMAFLRYVPVLDNSALTVRVRI
                     ILSLALAIIITPLIPHPIPHDLLSLNSLILTVEQILWGMLFGLMFQFLFLALQLAGQI
                     LSFNMGMSMAVMNDPSSGASTTVLAELINVYAILLFFAMDGHLLLVSVLYKGFTYWPI
                     GNALHPQTLRTIALAFSWVLASASLLALPTTFIMLIVQGCFGLLNRIAPPLNLFSLGF
                     PINMLAGLVCFATLLYNLPDHYLHLANFVLQQLDALKGHYGG"
     gene            11165..12304
                     /gene="flhB"
                     /locus_tag="PN977_002339"
     CDS             11165..12304
                     /gene="flhB"
                     /locus_tag="PN977_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016236920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FlhB"
                     /protein_id="HDI6042367.1"
                     /translation="MADSSSEEKTEKPSAQKLRKAREEGQLPRSKDMGLAASLFAAFV
                     VISSSFPWYADFVRESFISVHQYAQEINNPDVIGQFLRHHLLILGKFILTLLPMPAAA
                     LLSSLVPGGWLFLPKKILPDFSKISPLKGIGRLFSSEHLAETGKMTVKSVVVLVMLWV
                     SLRNNFAAFLGLQALPFKLAINDGLSLYASVMRNFVILFIFFALIDVPLAKALFTKGL
                     KMTKQELKEEYKNQEGKPEVKARVRRLQRQLAMGQIRKVVPKANVVITNPTHYAVALQ
                     YDQSRAAAPFVVAKGTDEIALYIRQVAAENQVEVVEFPRLARSVYYTTQVNQQIPFQL
                     YRAIAHVLTYVLQMKHWREGAQPRPALNRHISIPKEVLKLDGENN"
     gene            12288..14381
                     /gene="flhA"
                     /locus_tag="PN977_002340"
     CDS             12288..14381
                     /gene="flhA"
                     /locus_tag="PN977_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
                     /protein_id="HDI6042368.1"
                     /translation="MAKTTKSFLALLRGGNLGVPLVILCILAMVILPLPPALLDILFT
                     FNIVLAVMVLLVAVSAKRPLEFSLFPTILLITTLMRLTLNVASTRVVLLHGHLGAGAA
                     GKVIESFGQVVIGGNFVVGFVVFIILMIINFIVVTKGAERISEVSARFTLDAMPGKQM
                     AIDADLNAGLINQAQAQTRRKDVASEADFYGAMDGASKFVRGDAIAGMMILAINLIGG
                     VCIGIFKYNLSADAAFQQYVLMTIGDGLVAQIPSLLLSTAAAIIVTRVSDNGDIAHDV
                     RHQLLASPSVLYTATGIMFVLAVVPGMPHLPFLLFSALLGFTGWRMSKRPQAAEAEEK
                     SLETLTRTITETSEQQVSWETIPLIEPISLSLGYKLVALVDKAQGNPLTQRIRGVRQV
                     ISDGNGVLLPEIRIRENFRLKPSQYAIFINGIKADEADIPADKLMALPSSETYGEIDG
                     VLGNDPAYGMPVTWIQPAQKAKALNMGYQVIDSASVIATHVNKIVRSYIPDLFNYDDI
                     TQLHNRLSSMAPRLAEDLSAALNYSQLLKVYRALLTEGVSLRDIVTIATVLVASSAVT
                     KDHILLAADVRLALRRSITHPFVRKQELTVYTLNNELENLLTNVVNQAQQAGKVMLDS
                     VPVDPNMLNQFQNTMPQVKEQMKAAGKDPVLLVPPQLRPLLARYARLFAPGLHVLSYN
                     EVPDELELKIMGALM"
     gene            complement(14493..14990)
                     /locus_tag="PN977_002341"
     CDS             complement(14493..14990)
                     /locus_tag="PN977_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDI6042369.1"
                     /translation="MSEYRRYYIKGGTWFFTVNLRNRRSHLLTAQFQMLRKVIINVKR
                     ARPFEINAWVVLPEHMHCIWTLPESDDDFSSRWREIKKQFTHACGLQNIWQPRFWEHA
                     IRNTKDYRHHVDYIYINPVKHGWVKQVSDWPFSTFHRDVAKGLYPIDWAGDVTDFSAG
                     ERIIV"
     gene            complement(15166..15939)
                     /locus_tag="PN977_002342"
     CDS             complement(15166..15939)
                     /locus_tag="PN977_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308281.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HDI6042370.1"
                     /translation="MHDQPLLSMSLPSIPSFVLSGLLLLCLPFSSFASATTSHISFSY
                     AARQRMQNRARLLKQYQTHLKKQAGYIVEGNAESRKALRQHNREQIKQHPEWFPAPLK
                     ASDRRWQALAENNHFLSSDHLHNITEVAIHRLEQQLGKPYVWGGTRPDKGFDCSGLVF
                     YAYNKILEAKLPRTANEMYHYHRATIVANNDLRRGDLLFFHIHSREIADHMGVYLGDG
                     QFIESPRTGETIRISRLAEPFWQDHFLGARRILTEETIL"
     gene            16125..16385
                     /gene="dinJ"
                     /locus_tag="PN977_002343"
     CDS             16125..16385
                     /gene="dinJ"
                     /locus_tag="PN977_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin DinJ"
                     /protein_id="HDI6042371.1"
                     /translation="MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVARE
                     KALPFDLREPNQLTIQSIKNSEAGVGIHKAKDADDLFDKLGI"
     gene            16388..16665
                     /gene="yafQ"
                     /locus_tag="PN977_002344"
                     /pseudo
     CDS             16388..16665
                     /gene="yafQ"
                     /locus_tag="PN977_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414760.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YafQ"
     gene            16821..17561
                     /locus_tag="PN977_002345"
     CDS             16821..17561
                     /locus_tag="PN977_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein L,D-transpeptidase"
                     /protein_id="HDI6042372.1"
                     /translation="MRKIALILAMLLIPCVSFAGLLGSSSSTTPVSKEYKQQLMGSPV
                     YIQIFKEERTLDLYVKMGEQYQLLDSYKICKYSGGLGPKQRQGDFKSPEGFYSVQRNQ
                     LKPDSRYYKAINIGFPNAYDRAHGYEGKYLMIHGDCVSIGCYAMTNQGIDEIFQFVTG
                     ALVFGQPSVQVSIYPFRMTDANMKRHKYSNFKDFWEQLKPGYDYFEQTRKPPTVSVVN
                     GRYVVSKPLSHEVVQPQLASNYTLPEAK"
     gene            complement(17532..18299)
                     /locus_tag="PN977_002346"
     CDS             complement(17532..18299)
                     /locus_tag="PN977_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HDI6042373.1"
                     /translation="MCELLGMSANVPTDICFSFTGLVQRGGGTGPHKDGWGITFYEGK
                     GCRTFKDPQPSFNSPIAKLVQDYPIKSCSVVAHIRQANRGEVALENTHPFTRELWGRN
                     WTYAHNGQLTGYKSLETGNFRPVGETDSEKAFCWLLHKLTQRYPRTPGNMAAVFKYIA
                     SLADELRQKGVFNMLLSDGRYVMAYCSTNLHWITRRAPFGVATLLDQDVEIDFSSQTT
                     PNDVVTVIATQPLTGNETWQKIMPGEWRLFCLGERVV"
     gene            complement(18415..18993)
                     /gene="lpcA"
                     /locus_tag="PN977_002347"
     CDS             complement(18415..18993)
                     /gene="lpcA"
                     /locus_tag="PN977_002347"
                     /EC_number="5.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-sedoheptulose 7-phosphate isomerase"
                     /protein_id="HDI6042374.1"
                     /translation="MYQDLIRNELNEAAETLANFLKDDANIHAIQRAAVLLADSFKAG
                     GKVLSCGNGGSHCDAMHFAEELTGRYRENRPGYPAIAISDVSHISCVGNDFGFNDIFS
                     RYVEAVGREGDVLLGISTSGNSANVIKAIAAAREKGMKVITLTGKDGGKMAGTADIEI
                     RVPHFGYADRIQEIHIKVIHILIQLIEKEMVK"
     gene            19233..21677
                     /gene="fadE"
                     /locus_tag="PN977_002348"
     CDS             19233..21677
                     /gene="fadE"
                     /locus_tag="PN977_002348"
                     /EC_number="1.3.8.7"
                     /EC_number="1.3.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase FadE"
                     /protein_id="HDI6042375.1"
                     /translation="MMILSILATVVLLGALFYHRVSLFISSLILLAWTAALGVAGLWS
                     VWVLVPLAIILVPFNFAPMRRSMISAPVFRGFRKVMPPMSRTEKEAIDAGTTWWEGDL
                     FQGKPDWKKLHNYPQPRLTAEEQAFLDGPVEEACRMANDFQITHELADLPPELWAYLK
                     DHRFFAMIIKKEYGGLEFSAYAQSRVLQKLSGVSGILAITVGVPNSLGPGELLQHYGT
                     DEQKDHYLPRLARGQEIPCFALTSPEAGSDAGAIPDTGIVCMGEWQGQQVLGMRLTWN
                     KRYITLAPIATVLGLAFKLSDPEKLLGGAEDLGITCALIPTTTPGVEIGRRHFPLNVP
                     FQNGPTRGKDVFVPIDYIIGGPKMAGQGWRMLVECLSVGRGITLPSNSTGGVKSVALA
                     TGAYAHIRRQFKISIGKMEGIEEPLARIAGNAYVMDAAASLITYGIMLGEKPAVLSAI
                     VKYHCTHRGQQSIIDAMDITGGKGIMLGQSNFLARAYQGAPIAITVEGANILTRSMMI
                     FGQGAIRCHPYVLEEMEAAKNNDVNAFDKLLFKHIGHVGSNKVRSFWLGLTRGLTSST
                     PTGDATKRYYQHLNRLSANLALLSDVSMAVLGGSLKRRERISARLGDILSQLYLASAV
                     LKRYDDEGRNEADLPLVHWGVQDALYQAEQAMDDLLQNFPNRVVAGLLNVVIFPTGRH
                     YLAPSDKLDHKVAKILQVPNATRSRIGRGQYLTPSEHNPVGLLEEALVDVIAADPIHQ
                     RICKELGKNLPFTRLDELAHNALAKGLIDKDEAAILVKAEESRLRSINVDDFDPEELA
                     TKPVKLPEKVRKVEAA"
     gene            complement(21720..22166)
                     /gene="ivy"
                     /locus_tag="PN977_002349"
     CDS             complement(21720..22166)
                     /gene="ivy"
                     /locus_tag="PN977_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ivy family C-type lysozyme inhibitor"
                     /protein_id="HDI6042376.1"
                     /translation="MFKAITTVAALVIATSAMAQDDLTISSLAKGETTKAAFNQMVQG
                     HKLPAWVMKGGTYTPAQTVTLGDETYQVMSACKPHDCGSQRIAVMWSEKSNQMTGLFS
                     TIDEKTSQEKLTWLNVNDALSIDGKTVLFAALTGSLENHPDGFNFK"
     gene            22347..23117
                     /gene="yafV"
                     /locus_tag="PN977_002350"
     CDS             22347..23117
                     /gene="yafV"
                     /locus_tag="PN977_002350"
                     /EC_number="3.5.1.111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutaramate amidase"
                     /protein_id="HDI6042377.1"
                     /translation="MPGLKITLLQQPLVWMDGPANLRHFDRQLEGITGRDVIVLPEMF
                     TSGFAMEAAAASLAQDDVVNWMTAKAQQCNALIAGSVALQTESGSVNRFLLVEPGGTV
                     HFYDKRHLFRMADEHLHYKAGNARVIVEWRGWRILPLVCYDLRFPVWSRNLNDYDLAL
                     YVANWPAPRSLHWQALLTARAIENQAYVAGCNRVGSDGNGCHYRGDSRVINPQGEIIA
                     TADAHQATRIDAELSMVALREYREKFPAWRDADEFTFR"
     gene            complement(23216..23292)
                     /locus_tag="PN977_002351"
     tRNA            complement(23216..23292)
                     /locus_tag="PN977_002351"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(23256..23258),aa:Asp,seq:gtc)
     gene            complement(23425..24156)
                     /gene="dnaQ"
                     /locus_tag="PN977_002352"
     CDS             complement(23425..24156)
                     /gene="dnaQ"
                     /locus_tag="PN977_002352"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HDI6042378.1"
                     /translation="MSTAITRQIVLDTETTGMNQIGAHYEGHKIIEIGAVEVVNRRLT
                     GNNFHVYLKPDRLVDPEAFGVHGIADEFLLDKPTFAEVADEFMDYIRGAELVIHNAAF
                     DIGFMDYEFSLLKRDIPKTNTFCKVTDSLAVARKMFPGKRNSLDALCARYEIDNSKRT
                     LHGALLDAQILAEVYLAMTGGQTSMAFAMEGETQQQQGEATIQRIVRQASKLRVVFAT
                     DEELAAHEARLDLVQKKGGSCLWRA"
     gene            24221..24688
                     /gene="rnhA"
                     /locus_tag="PN977_002353"
     CDS             24221..24688
                     /gene="rnhA"
                     /locus_tag="PN977_002353"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HDI6042379.1"
                     /translation="MLKQVEIFTDGSCLGNPGPGGYGAILRYRGREKTFSAGYTRTTN
                     NRMELMAAIVALEALKEHCEVILSTDSQYVRQGITQWIHNWKKRGWKTADKKPVKNVD
                     LWQRLDAALGQHQIKWEWVKGHAGHPENERCDELARAAAMNPTLEDTGYQVEV"
     gene            complement(24685..25407)
                     /locus_tag="PN977_002354"
     CDS             complement(24685..25407)
                     /locus_tag="PN977_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414749.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDI6042380.1"
                     /translation="MKPARVPQTVVAPDCWGDLPWGELYRKALERQLNPWFTKMYGFH
                     LLKIGNLSAEINCEACAVSHQVNVSAQGMPVQVQADPLHLPFADKSVDVCLLAHTLPW
                     CTDPHRLLREADRVLIDDGWLVISGFNPISLMGLRKLVPVLRKTSPYNSRMFTLMRQL
                     DWLSLLNFEVLHASRFHVLPWNKHGGKLLNAHIPALGCLQLIVARKRTIPLTLNPMKQ
                     SKNKPRIRQAVGATRQCRKPQA"
     gene            25441..26196
                     /gene="gloB"
                     /locus_tag="PN977_002355"
     CDS             25441..26196
                     /gene="gloB"
                     /locus_tag="PN977_002355"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HDI6042381.1"
                     /translation="MNLNSIPAFDDNYIWVLNDEAGRCLIVDPGDAEPVLNAIAANNW
                     QPEAIFLTHHHHDHVGGVKELVKKFPQIVVYGPQETQDKGTTQVVKDGETAFVLGHEF
                     SVIATPGHTLGHICYFSKPYLFCGDTLFSGGCGRLFEGTASQMYQSLKKLSALPDDTL
                     VCCAHEYTLSNMKFALSILPHDLSINDYYRKVKELRAKNQITLPVILKNERQINVFLR
                     TEDIDLINVINEETLLQQPEERFAWLRSKKDRF"
     gene            26268..27626
                     /gene="mltD"
                     /locus_tag="PN977_002356"
     CDS             26268..27626
                     /gene="mltD"
                     /locus_tag="PN977_002356"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase D"
                     /protein_id="HDI6042382.1"
                     /translation="MKAKAILLASVLLVGCQSTGNVQQHAQSLSAAGQGEAAKFTSQA
                     RWMDDGTSIAPDGDLWAFIGDELKMGIPENDRIREQKQKYLRNKSYLHDVTLRAEPYM
                     YWIAGQVKKRNMPMELVLLPIVESAFDPHATSGANAAGIWQIIPSTGRNYGLKQTRNY
                     DARRDVVASTTAALNMMQRLNKMFDGDWLLTVAAYNSGEGRVMKAIKTNKARGKSTDF
                     WSLPLPQETKQYVPKMLALSDILKNSKRYGVRLPTTDESRALARVHLSSPVEMAKVAD
                     MAGISVSKLKTFNAGVKGSTLGASGPQYVMVPKKHADQLRESLASGEIAAVQSTLVAD
                     NTPLNSRVYTVRSGDTLSSIASRLGVSTKDLQQWNKLRGSKLKPGQSLTIGAGSSAQR
                     LANNSDSITYRVRKGDSLSSIAKRHGVNIKDVMRWNSDTANLQPGDKLTLFVKNNSMP
                     DS"
     gene            complement(27675..28445)
                     /locus_tag="PN977_002357"
     CDS             complement(27675..28445)
                     /locus_tag="PN977_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HDI6042383.1"
                     /translation="MTTQSHHDHVEKQFSSQASEYLTSTVHASGRDLQRLAVRLADYP
                     DASVLDMGCGAGHASFVAAQNVSAVVAYDLSAQMLDVVAQAAEARQLKNITTRQGYAE
                     SLPFADNAFDIVISRYSAHHWHDVGAALREVNRVLEPGGRLIVMDVMSPGHPVRDIWL
                     QTVEALRDTSHVRNYASGEWLKLINEANLIVDNLITDKLPLEFSSWVARMRTPEALVD
                     AIRIYQQSASTEVKTYFALQNDGSFTSDIIMLEAHKAA"
     gene            complement(28519..29319)
                     /locus_tag="PN977_002358"
     CDS             complement(28519..29319)
                     /locus_tag="PN977_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HDI6042384.1"
                     /translation="MRKNTYAMRYVAGQPAERILPPGSFASIGQALPPGEPLSTEERI
                     RILVWNIYKQQRAEWLSVLKNYGKDAHLVLLQEAQTTPELVQFATANYLAADQVPAFV
                     LPQHPSGVMTLSAAHPVYCCPLREREPILRLAKSALVTVYPLPDTRLLMVVNIHAVNF
                     SLGVDVYSKQLLPIGDQIAHHSGPVIMAGDFNAWSRRRMNALYRFAREMSLRQVRFTD
                     DQRRRAFGRPLDFVFYRGLNVSEASVLVTRASDHNPLLVEFSPGKPDK"
     gene            29560..30474
                     /locus_tag="PN977_002359"
     CDS             29560..30474
                     /locus_tag="PN977_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDI6042385.1"
                     /translation="MKATSEELAIFVSVVESGSFSRAAEQLGQANSAVSRAVKKLEMK
                     LGVSLLNRTTRQLSLTEEGERYFRRVQSILQEMAAAESEIMETRNTPRGLLRIDAATP
                     VVLHFLMPLIKPFRERYPEVTLSLVSSETIINLIERKVDVAIRAGTLTDSSLRARPLF
                     NSYRKIIASPDYISRYGKPETIDDLKQHICLGFTEPASLNTWPIACSDGQLHEVKYGL
                     SSNSGETLKQLCLSGNGIACLSDYMIDREIARGELVELMADKVLPVEMPFSAVYYSDR
                     AVSTRIRAFIDFLSEHVKTAPGGAVREA"
     gene            complement(30471..31274)
                     /gene="dkgB"
                     /locus_tag="PN977_002360"
     CDS             complement(30471..31274)
                     /gene="dkgB"
                     /locus_tag="PN977_002360"
                     /EC_number="1.1.1.346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgB"
                     /protein_id="HDI6042386.1"
                     /translation="MAIPAFGLGTFRLKDDVVISSVKTALELGYRAIDTAQIYDNEAA
                     VGQAIAESGVPRNELYITTKIWIENLSKDKLTPSLKESLQKLRIDYVDLTLIHWPSPN
                     DEVSVEEFMQALLEAKKQGLTREIGISNFTIPLMEKAIAAVGAENIATNQIELSPYLQ
                     NRKVVAWAKQHGIHITSYMTLAYGKALKDEVIARIAAKHNATPAQVILAWAMGEGYSV
                     IPSSTKRKNLESNLKAQNLQLDAEDKKAIAALDCNDRLVSPEGLAPEWD"
BASE COUNT         7389 a         8147 c         8244 g         7654 t
ORIGIN      
        1 taacgctatg tttgttatgt atggttgtcg cactagttat gcaagtgatg attcaggaca
       61 aggttttgcc agtaaattat ccctattaga taattttaag gacgttaatg tctggggaca
      121 aacagaaagt acttatccat cttatttccc agatattagg acaacttcga ttatgaggtc
      181 aataaatata ggttggtctt tttcaccaac atacatggtt gccagttctg aaggtcaagg
      241 atgggatgcc ttatttccgg atgataaaaa tccactaaag tctctcccta tgcagtgtta
      301 taataatggg aaattgattt taacctgcga tcaaagcagt tttaatgatc accggaagaa
      361 taaatcaaat gattagtatt atattgagaa gcttgattct attattcatg ataatagtgt
      421 atccagatta taataaaaac ctacctgcct ttgaagtata tggggaaaat gacatactta
      481 aggatatggt tattaatatg aaaacttata gtaaccatag taagaaaaac tcttccccaa
      541 caactatagt gcaaaccatc ctttttaaag ggaatataca gcaagttttt gaaagcaacg
      601 atatgggtgc agcgagcttc tttttttctt ataataacaa ggttgaaacg ctggtaacag
      661 acgctttctt tttgaataga aaattagaca aactgcatga tgcaaatatt tttgaagtta
      721 taaagcatgg ggaaaatatc gttattagat attacaaatg caaaattgaa tatatgaaaa
      781 aagaaaaatc tatcgaaata gagatatcaa caaacaaaaa cagttatttt agcaaaaatg
      841 gaataaaaca aaaagacaca aataaatata acacatattt cttcaattac tttgaggctc
      901 aaaaataaat tttattttgt catgctttcc ccctaatcac ctcaataatc cccgtcgtcg
      961 acaccgacgg cgtgcggggg aaatacacta cctcacaaat atacgcgaag ctgtcaaact
     1021 ttcctgccca gtcatcgccc atcactaaaa tatccgcact gtacccccgc agatactccg
     1081 ctttctgctc cagcgactct tccagaaata cgccatccac gcacgccagc ccggcgatga
     1141 tcgccatgcg gtcatcctga gggtaaaccg gtgccctgcc ctttttggcg atgttcaggg
     1201 catcggacga tacgccgacc agcagccgtt cgcccagcgt gcgggcgcgt tgtaaaaggc
     1261 gcagatgacc gacatgaaac acatcgaagg tgccgaaggt gatgaccgtt ttcatggcta
     1321 ctccgctaac ttatgccgca gccagcactg gacggcgatg tcatccaccg ctttttgcgc
     1381 tttcgccgtg gcactttctt gctgctcgcg gcgtttgtcg tccagcgtca gggccaccac
     1441 tttctcttcg cacgccgccg ccgtcaggtt tttctgcatt cgcgtggctt tgatgttagc
     1501 cagcgttttt tcctgctcct gccaggcgat caccttgcgc aaggtgcctt tgtagtcgct
     1561 gacgtttttc agcgattcca ccgacgctgc cgcgcccgca gatgttttct ccaccaggta
     1621 gcctaatgct ttgatattgt tgtcataacc cgcacaaatc tgtttttgct ttgccagctc
     1681 gacggttttg tctttgaccg atttatcccg caggcgctgc aactgcgcca gggtgtcgat
     1741 gatttgtcgc atggttactg ttctgccttt ttgacgccag gaaagagaat ttgcaggcgg
     1801 ctttgcacca gttcgaggga ggcgggttcg cgcatctcct ggcgcaaaaa acgctcgata
     1861 gccgggaaca ttttcaccgc tttgtcgacg ctggcgtccg cgcctgccac ataaccgccg
     1921 agtgggatca gcggtttgat ctccatgtag gcggcgtagt tttgctttaa cgcgcgggcg
     1981 gattgctgat gttccaggct ggtgacctgg ttcatgcagc ggctgatcga ctggccgata
     2041 tcaatggccg gataatgccc cgcttccgcc agtttgcggg ttaatacgat atgcccgtcc
     2101 agcaccgcac gggcgcagtc gacaatcgga tcttgctggt cgtcgccttc cgccagcacg
     2161 gtgtagatgg cagtcatcga tcccgcgctt tcgctgttgc ctgcgctttc gaccagtttg
     2221 gggatcatgc cgaatgccga cggcggatag cctttggtgg cgggtggttc gccaagcgag
     2281 agcgcaattt cacgctgcgc catcgcatag cgggtcaggg aatccaccag cagtaaaacg
     2341 tgatgcccgc ggtcgcgaaa ccaggcggcg acggagtggc acagttcggt ggctttcagg
     2401 cgcatcagcg gcgattcatc ggcgggcgcg acgacgacga tagacttcgc cagcccgtcc
     2461 gcgcccagtg agtggtcgat aaactctttc acttcgcgcc cgcgttcgcc aatcagcccg
     2521 accaccacga tgtcggcttt ggtctggcgg gtgatcatcc ccagcagcac gcttttcccg
     2581 acgccgctgc ccgccatcag cccgacacgc tggcctttgc cgatggtcag caggccgttg
     2641 atcgctttca cgcccacgtc cagcggttcg tccaccgaac ggcgggttaa gggattaacc
     2701 gacggcggca gcggcggcag aaggtcgttg ccgttcaggc ggcctttgcc atccagcggt
     2761 tcgccgaggc cgttaaccac gcggcctaac cagctttcgc caatcaggat gtcgtgggct
     2821 ttttcttccg ggaagacgcg cgcccccgcc atcaggccga ccggctgttt aaacggcatt
     2881 aagtaagtga tatcgcgatt aaagcctacg gcctgcgcgt cgataagcgt gtggttggcg
     2941 ctttcgatgc gacacagctg cccggtcatt agcgggcagc cgacgctctc cagcaggatg
     3001 ccattaaggc gcaccagccg cccggcgacg cgggccagcg ggatcgactc cagcgagcgc
     3061 agggcgttat cgaaagcgga aaaatcactc accctgtgac tccggcgtta gcgtctcttg
     3121 tagcgcggtc atgcattgtt ccaggcggtg ctcgcagccg atatccagtt cggatttatc
     3181 ggtgatcacc cggcattcac ccggcggcag atccggcgaa ggcgtcagcc cccattcgct
     3241 gaccttttcc ggcaccgcgt cgcgcaagcg gttaaattct tccgtactta gcatcacttg
     3301 gagggtctcc ggcattgccg ggaaggccgc cagtgcctct tccaccagcg ccagcaactg
     3361 cgtcggttgc agcgccagtt cgcagcggat cacctggcgg gtgacttttt ccaccagttg
     3421 cagcaaatct tcccgctgtt tgcgttcgat atgggcgaga aagtcgttca cttttccgct
     3481 gatggcttcc agcggctgtg ccgcgtcaac aaactgtttg cgcccttcct gctgcccggc
     3541 gaggctgcct tcggtgtagc cctggcgtcg cccctcggca aaaccttgtt gatgaccttc
     3601 gctaaagccc tgctcctggc cttcggtcat tccctgagca aacccttttt gcagcccttc
     3661 ctgaaaaccg tccatcaact ggcgctggta atcggcggcg ctaatgctgg cggtgagcgg
     3721 ttctccggtc accgcacgct ggcgcggcgg aaaacggtgc aggcgatagc gcccgcgcag
     3781 tgtttcaatg gtcatggtta ctccgccgtc tgttccgcaa agagctgtag ctgaatttcg
     3841 cccgcctctt ccagttcacg ggcaatgccc atgatttcgc ggcggatctg ttcgatacgg
     3901 ctgaccggca ccggccccag acgcgcggtg atcgcttcca gttgctgcgc ctgacgtttt
     3961 ggcatcaccg cgtaaatgga acggcgcaac agggcttcgg tgcctttcag cgctaccgcc
     4021 cagtcttcca tcggtacttc gtcgagcaga cgtcgacgca cctcttcgtt ctggcggctg
     4081 aggataaaga agtcgtacat ctcatcctga agctgctcca gcacgtcttc gtcgcgctcg
     4141 cgcatctggt cgaggatcac ctgctggttg ccctggaagc ggttgacgat atccgccgcc
     4201 tgtttgatgc ctttcacctt cgagccgtgc tccgaaagca ccgacaggcc gcgttcaatc
     4261 agcctgtcca gttcatccac cacgtcgcgg ttgacgtcgt tgagtttcgc cacccgatag
     4321 agaatttcgt tttgcaccga ttcattgaga tacgagagca ccgccgccga gatttccggc
     4381 gtcagaaacg cgaggaatac tgcctgcaat tgcagatgct cttcggagat cagcatcgcc
     4441 agctggcgcg gttccaccca ttgcagtcgc gccatccgcg agcggatctc atcgccgtag
     4501 atgccgttga tcacgctgct ggcgatctcg gtgcctagcg ctttgttgag aatgccctgc
     4561 aacatagatc gcgacgcgcc attgatcccg ctctgttcgc ggaactcatc aaagaagttg
     4621 ttgatcactt tgcgcgccat gctggttttg acaccggaaa gccgcgccat gttttcgctc
     4681 aggcgcacca cttcttcgcg gctgagtttt tgcatcaccg tcgccgccgc ttcttcgcca
     4741 aggcacagta gcaggatcgc cgcctgttcc aggtagctgt tgctcttatt tgttgtcaat
     4801 tcgctcattg ctggtgatcc attgtctgag aacttcggct acgcgatcgg tgtcgctcat
     4861 ggccagtttt tgcaggaact cgagttttac ttcgaggccg gaactctggg acggcaggct
     4921 gtcatcgccg ggcagcgacg gcagatcgac gtttttgcgt tcctcttccg gcatggcaat
     4981 ccacggttcg ctgacagctt ccggttctgg tgtgataacc ggcgcacgta ccgccgtcag
     5041 ccgtttcatt accgggcgca ccacaaagaa cagcaacaac agcgccagca ggccgcagcc
     5101 aatcagccgc acccaggcca gcacgctgtc gtctttccac agcggaataa ttggttcgac
     5161 cggcaccgcc tgcggcacaa agttaagcag cgataaggtg agattgtcac cgcgctggac
     5221 gtcgatcccg gcggcattgt tcagcaacgc ggtcagttgc gtggtctgct ccggcttcca
     5281 gtttttcagc gccggagcgt tttgattgag caccaccgcc acgttaaggc gtttcaccgc
     5341 aaagccggga tgctgaatat gctggacgct gcggtcgtaa gagtaatcgc gcttgctttc
     5401 gctgtgtttc gaaagggctt ccggcgagcg gttttcttcc gtaccgttgg tcatctgatt
     5461 cgctgcaatc ggcggacggt tactgagcga accgggcaca cccatcgcca cctgattggt
     5521 atcgctgtcc agcacgtttt cttcgcggtt gattttcggc gcgtcgccgt agtgttcctg
     5581 agtttcatcg atattactga ggtcgagatc cggcatgacg ctgacgcggt aattcccgct
     5641 gccgaccagc gaatccagca cgttcgccac gctggcgcgg gttttgtcct gaatatcttt
     5701 gaggatctga tcgcgtttac gggtagcggc tgaaaccgct tcgcccgcgc caatgccgtc
     5761 ggtcagcaga tttcccgcct gatcgacgac gctaactttg ctggcgtgca gccccggaat
     5821 gctgccggaa accagatgca caatggcgtt cacctggtcc atattcagct tcgcgccgta
     5881 atgcagacgc accaccaccg aggcgctgtt ttgcggctca tcgctgacca caaacgagct
     5941 ctcttcgttg agcgccagat gcacccgcgc gctctccacc gcgtccagcg acataatgct
     6001 ctgcgccagc tccccttcga ggctgcgttt gtagcgcacg ttctgcacga actggctgct
     6061 gccgagcact tcgtctttgt ccatcagctc gtagccgctg ggcaagatcg cctgcacgcc
     6121 tttcgcggca agggtcatac gcgttttcga cagttcatct tccggcacca gaatctgccc
     6181 gctttgcggg tcgatgcggt agctgagctt ttcgccgtcc agcacggtga caatctgcga
     6241 cacgggcaga ttttcctggc gtccgtagag cgagacgtag ccgcgattac cggtccacag
     6301 tacgctgacg ataattgccg tcgccgcaac ggcaagcccc actcccgcca tcaatgccca
     6361 gcgtttgtta tctgcaagac gcagtcgaaa cgccggaaac gcctgggtta acttctttat
     6421 ttgtgcattc atagggttag atcgacatac tcatcagatc gttaaatccg gtggcgattt
     6481 tgttgcgcac ctgcaccagc gcggagaaag agagcgacgc ctgctggctg gcgatcatcg
     6541 ccccggcgag gtcatcactt ttgcccgtgt cgacagccgt ttgctgctgc tccgccacct
     6601 gctggaactg gtcaacgtga ccgagcgcgc cggacataat acggttaaac gacacgtccg
     6661 ggtcggtgga actgaaggtc atggcgggta gcactggcgc ctgggcgttg gcctgcatcc
     6721 gctgcacgtc ctgcataatt tgcgcctgca tggtgctgtt gattgtggta atgctcattt
     6781 ttaacttccg ggtcaggaga aaacttcgat accgtgcttg cgcatggagg ccagtcgata
     6841 gcgcagtgcg cgcggggtaa tgcctaacag gtcagcgatt ttgctgcggt tgccctgata
     6901 tttacgcatc aggtcggcga tatattgata ctgcgcgctt cgtccgtgct ggcctaaatc
     6961 gccggtttcg gcaatgtgga cggcgggctg aacaggccat tgcagttcac tgtggtcagc
     7021 aatatcggtg tccggcaggc ctaatgcatc cgggtaaatt acgccgtcgc gatttaaaat
     7081 cattccccgc tgaatggcgt tttccagttg gcgaacgtta ccgggccaac ggtaattgag
     7141 cagcgcacgg cgggtggatt cggacagggt gatatttttc accagcaccg tggagtattt
     7201 tttaataaat gactctgcca gcggaataat atcgttcaga cgttcacgca gcggcggcat
     7261 ggtaatagga atcaccgcca gacgataata gagatcttca cgaaaacgtc cggcggcaac
     7321 ttcctgctcc aggtttttat tggtgcaggc aatcaggcga aaattgagtt tgatttgtcg
     7381 gttgctgcct aatcgctcaa cctgttgttc ctgcaacacg cgtaatattt tggcctgtag
     7441 cgccagcggc atttcaccaa tttcatcgag caataaggag ccgttatttg ccagttccat
     7501 ttttcctggc acgctggcaa ttgcgccggt aaatgcgcct ttgtcgtagc caaataacgt
     7561 cgattccagc atattttccg ggatcgccgc gcagttcacg ccgatatagg gcgcattatc
     7621 attttcacca aaggctacgg tgtgaatata tttcgccacg cactctttcc ccgcgccggt
     7681 ttcgccctga atgagcaccg gcacgttaaa ggcggcgaca cgctttgcca gagtaaaagc
     7741 gttaatgctg gaagcggcag ttgcaataag ttcagacata gggtgaacaa tataagtagc
     7801 gacaataatc aggatgttct gaagcgtatc accggcggga ttaacaagtt cgaattaaac
     7861 gataataaaa acattatttg atggtaaaca attagttaga tattaatact gttaatttgg
     7921 acatctgtga tgccccctca ccctaaccct ctccccggat ggggcgaggg gacagaccgc
     7981 agactgattg gcgaaaatca ctcccattaa gcaattaatc gattaattat tttttcaccc
     8041 tattaataat ttgccccttt cactccccct ctatttaata cctcaccttg tgtcacagcc
     8101 ggatttgcgt gcacagaaac acccatacca ttacagcaga gaaagtaagc catgctgaag
     8161 tacagtaaaa cgccaggcat ctttaaatta gaaggtaatc ggctcggacg cccctaccat
     8221 cgcctgccga ccctgtttac ggggaatttt gacgtgattg attcgcatct tggcagctac
     8281 tttctcaaaa agcatcgcag caatatcacc ttaaaaaaga ttggctgcga aatggatatt
     8341 attaataaaa acgccgaact gatggtttcc caggtcgggc atctggcgtt tgatattgat
     8401 cgctccttat tgttaatgct gctgggcaat ttttatggtc tggagtcctc tcttgaagag
     8461 gcgaaagcgc ataacgggct gccgaccaaa accgaaactc gtttaaaaaa ccgactcgcg
     8521 ctggatatct gcacgctgat atttaatctg caaacctccg gtattgcgct gaaattaaaa
     8581 ctggacagca gcacggtgat tacccactgg gcgtatcaat taacctttac ccttgcgggc
     8641 gatgaagaga gcagtttccg tattttgctc gacgacgccc acaccgattt tattttgaat
     8701 ctgatccgcc acagcgagca cagtaaaccg cagcaggtgg cgaaatccgt taataaaccg
     8761 gccctgatta aagaaatcat ccgctccctg ccgctgaccc tgaacgtcaa aatcgccgag
     8821 ctatcgatga atgtcgccga tctggcgcag ataaaagccg gggatatttt acccatctcg
     8881 ctgggagaaa cgttcccggt ggcgattggg caatctgaat tatttagcgc cctgattgtg
     8941 gaagacaaag acaaactgtt tttgtcggag ctggcgggca agaatgagaa tagccatgag
     9001 taaacaagac gatattttag ccggggattt tggcctcgcc gatgaagttg cgcctgtcac
     9061 gaaatccgcc gacgccgaaa cgctggtcac ccgcctggaa gaccgctttt ccgactcgat
     9121 gacgctgctg aaacgcattc cggtgacctt aacgctggaa gtgtcgtcgg tggaaattat
     9181 gttagccgac ctgcttaaca tcgacgacga cacggtgatt gagctggata agctcgccgg
     9241 ggagccgctg gatatcaaag tgaacaatat cctgctgggc aaagcggaag tggtggtggt
     9301 caacgagaag tacggcctgc gcgtgctgga gtttaacacc cgcgatatca acgacctggc
     9361 accatgaaac gccgcactca actggcgctg ggccttgggc tgctggcgct tgcgccgctg
     9421 gcgttggccc agggtggcga tattgcgcta cttaacgtgg tgacccacgg caacactcag
     9481 gagtacagcg tcaaaattca ggtactgatt ctgatgaccc tcgtcggttt gctgccgacg
     9541 atggtgctga tgatgacctg ctttacgcgc tttattatcg tgctgtcgct gctgcgccag
     9601 gcgctggggc tgcaacaaac gccgccaaac cgcattctga ttggtattgc gctctcactc
     9661 actatgctgg tgatgcgccc tgtctggctc aatatttacg accacgcggt agtgccgttt
     9721 gaaaacgatc agattaccct gaccgatgcg ttaagcaccg ccgcgacgcc gctaaaacgc
     9781 tttatgctgg cgcagaccga caaaaaagcg atggcgcaaa tcatgaccat cggcggcgcg
     9841 aagggcaatg ccgccgacca ggatctcacg attgtggtgc ccgcctatgt gttgagcgag
     9901 ctgaaaaccg ctttccagat tggctttatg atttacatcc cgtttctggt tatcgacctg
     9961 attgtcgcaa gcgtactgat ggcgatgggg atgatgatgc tgtcgccgct gattgtttcg
    10021 ctgcccttta aactgatgct gtttgtgctg atcgacggct ggtcgctgac catcggcacg
    10081 ctcaccacca gcattcgcgg gctggggctg ggctgattat gttgaccgta gacgtagccg
    10141 ccgacattgt cgccagtggc ataaaagtgg tgattttgct ggtttcggtg ctggtggtgc
    10201 caagcctgct ggtcggcctg ctggtgagcg tgttccaggc ggtgacgcag attaacgaac
    10261 agaccctgag ctttttgccg aggctgattg tcacgctggt ggtgctgggg gtgtgcggca
    10321 aatggatgat tatccagttg catgatctct gcattcatct gtttagtcag gccgccctgc
    10381 tggtgcatta agatgcgcac aagcgacgtt acgcaactga cggatttggc gctggggctg
    10441 tggtttccct tcgtgcgcat tatggcgttt ctgcgctatg tgccggtgct ggataacagc
    10501 gccctgaccg tgcgtgtgcg gattattttg tcgctggcgc tggcaatcat catcacgccg
    10561 ttaattccgc atcccattcc ccacgatctg ctatcgctca acagtctgat cctgacggtg
    10621 gagcaaattt tatggggcat gttattcgga ttgatgtttc agtttctgtt tctggcgctg
    10681 caactcgccg ggcaaattct ctcgttcaat atggggatga gcatggcggt gatgaacgac
    10741 ccaagcagcg gcgcatcgac caccgtgctg gcggagttaa ttaacgtcta tgcgatcctg
    10801 ctgttttttg ccatggacgg gcatctgctg ctggtaagcg tgctgtataa gggtttcacc
    10861 tactggccga ttggcaacgc gctgcatccg caaaccctgc gcaccatcgc gctggccttt
    10921 agctgggtgc tggcgtccgc atcactgctg gcgctgccga ccacttttat catgctgatt
    10981 gtgcagggct gctttggcct gcttaaccgc attgcgccgc cgctgaacct tttttcgctc
    11041 ggctttccca tcaacatgct ggcggggctg gtttgcttcg ccaccctgct ctacaacctg
    11101 ccggatcact atctgcatct ggcgaatttt gtgttgcagc aactcgacgc gctgaaaggt
    11161 cactatggcg gatagcagca gcgaagaaaa aacagagaaa ccctcggcgc aaaagctgcg
    11221 taaagcgcgt gaagaggggc aactgccgcg ctcgaaggat atggggctgg cggcgagcct
    11281 gtttgcggcg tttgtggtga tctccagcag cttcccgtgg tatgccgatt ttgtgcgtga
    11341 gagttttatc agcgtgcatc agtacgcgca ggagattaat aacccggatg tgatcggcca
    11401 gtttctgcgc catcacctgt taattctcgg caagtttatt cttaccttgc tgccgatgcc
    11461 cgccgccgca ctgctctcct cgctggtgcc tggcggctgg ctgtttctgc cgaaaaaaat
    11521 cctccctgat ttcagcaaaa ttagcccgtt aaaagggatt gggcgactgt tttccagtga
    11581 gcatctggcg gaaacgggaa agatgacagt gaagtcggtg gtggtgctgg tgatgctgtg
    11641 ggtaagcctg cgcaataact ttgccgcctt tctcggcctg caggcgctgc cgttcaaact
    11701 ggcaattaac gatggtttat cgctctacgc cagcgtgatg cgcaactttg tcatcctgtt
    11761 tatcttcttt gcgcttatcg acgtgccgct ggcgaaagcc ctgtttacca aagggctgaa
    11821 gatgaccaag caggagctga aagaagagta caaaaatcag gaaggcaagc cggaagtgaa
    11881 ggcgcgcgtg cgacggctgc aacggcagct ggcgatgggg caaatccgca aagtggtgcc
    11941 gaaagccaac gtggtgatca ccaacccgac ccactacgcc gtggcgttgc agtatgacca
    12001 gtctcgcgcc gccgcgccgt ttgtggtggc aaaaggcacc gatgaaatcg ccctctatat
    12061 tcgccaggtc gccgctgaga atcaggtgga agtggtcgag ttcccgcggc tggcgcgctc
    12121 ggtttactac accacgcagg tgaaccaaca aattccgttt cagctttacc gggccatcgc
    12181 ccacgttctg acctacgtcc tacaaatgaa acactggcgc gagggcgcac agccgcgccc
    12241 ggccctgaac agacatattt ccattcccaa agaggtgctt aaactggatg gcgaaaacaa
    12301 ctaaatcgtt cctcgcgctg ctgcgcggcg gcaatctcgg cgtgccgctg gtaatactgt
    12361 gtattctggc gatggttatt ctgccgctgc cgcctgcgct gctggatatt ctgttcacct
    12421 tcaacattgt gctggcggtg atggtgctgc tggtggcggt gtcggcgaaa aggccgctgg
    12481 aattcagcct gttcccgacc attttgctga tcaccacttt aatgcgcctg acgctcaacg
    12541 tcgcctccac gcgcgtggtg ctgttacacg ggcatctcgg cgcgggcgcg gcgggtaagg
    12601 taattgagtc gtttggtcag gtggtgattg gcggcaactt tgtcgtcggt ttcgtggtgt
    12661 ttatcatcct gatgatcatc aactttatcg tcgtcaccaa aggggccgag cgtatttccg
    12721 aggtttctgc ccgctttacc ctggacgcga tgcccggtaa acagatggcg attgacgccg
    12781 atcttaacgc cggattgatc aaccaggcgc aggcacaaac ccggcgtaaa gatgttgcca
    12841 gcgaggccga tttctacggc gcaatggacg gggcatcgaa gtttgtgcgc ggcgatgcca
    12901 tcgccgggat gatgattctg gcgatcaacc tgatcggcgg cgtctgtatc gggatcttca
    12961 aatacaacct gagcgccgac gccgccttcc agcaatatgt gctgatgact atcggcgacg
    13021 ggctggtggc gcagatcccg tccctgctgc tctccaccgc ggcggcaatt atcgtcaccc
    13081 gcgtcagcga caacggcgat atcgcccatg acgtgcgcca ccagctgctg gcaagtccgt
    13141 cggtgctcta caccgccacc gggatcatgt ttgtgctggc ggtggtgccg gggatgccgc
    13201 atctgccatt tttgctgttc agcgccctgc ttggctttac cggctggcgg atgagcaaac
    13261 gcccgcaggc ggcggaggcg gaggagaaaa gcctcgaaac gctgacccga accatcaccg
    13321 aaaccagcga gcaacaggtc agttgggaaa ccattccgct gatcgagccc atcagcttaa
    13381 gcctcggcta caagctggtg gcgctggtgg acaaagcgca ggggaacccg ctcacccagc
    13441 ggattcgcgg cgtgcggcag gtgatatccg acggtaacgg cgtgctgctg ccggagatcc
    13501 gtattcggga aaacttccgt ctcaagccca gccagtacgc cattttcatc aacggcatta
    13561 aggctgatga agcggatatt ccggcggata aactgatggc cctgccctcc agcgaaacct
    13621 acggcgagat tgacggcgta ctggggaatg acccggcata tgggatgccg gtcacctgga
    13681 ttcagcctgc gcagaaagcg aaggcgctga atatggggta tcaggtgatc gacagcgcca
    13741 gcgtgattgc tacccatgta aataagattg tgcgcagcta tattcctgat ttatttaact
    13801 atgacgatat tacgcagttg cataatcgtt tgtcgtcgat ggcaccgcga ctggcggaag
    13861 acttaagcgc ggcgctcaat tacagccagt tgctgaaagt gtatcgcgcg ctgctgaccg
    13921 aaggcgtttc cctgcgcgat atcgtcacca tcgccaccgt gctggtcgcc agtagtgcgg
    13981 tgactaaaga tcatatcctg ctggcggccg atgtgcgcct ggcgctgcgg cgcagtatca
    14041 cccatccgtt cgttcgcaag caggagctga cggtgtatac gctgaataat gagctggaaa
    14101 atctgctgac caacgtggtg aatcaggcgc aacaggccgg caaagtgatg ctcgacagcg
    14161 tgccggtgga cccgaatatg cttaaccagt tccagaacac gatgccgcaa gtgaaggagc
    14221 agatgaaagc ggcggggaaa gaccctgtgc tgctggtgcc accgcagttg cgccctttgc
    14281 tggcgcgcta tgcgagattg tttgcgccgg ggctgcatgt gctgtcgtat aacgaagtgc
    14341 cggacgagct ggagttgaag attatggggg cgttgatgta ggtatccggc atctgcgcac
    14401 aaccgccgga tgcggcgtga acgccttatc cggcctaccg ttcaggcacg attcgtaggc
    14461 taagcgtaag cgcatccggc atcggcgcgc aattatacaa tgatacgctc cccggcacta
    14521 aaatccgtta cgtcccccgc ccaatcgatg ggatataacc ccttcgcgac atcgcgatga
    14581 aacgttgaga atggccaatc actcacttgc tttacccagc catgttttac tggatttata
    14641 taaatataat caacatgatg gcgatagtct ttggtattgc ggatggcgtg ctcccaaaaa
    14701 cgcggttgcc agatattttg caatccacaa gcatgggtga actgtttttt aatctcccgc
    14761 cagcgagagg aaaaatcatc atcgctttcg ggtaatgtcc agatacagtg catatgctcc
    14821 ggcaaaacta cccaggcgtt aatttcaaaa ggtctggctc gcttaacgtt aataatgact
    14881 ttacggagca tctggaactg agcggtcaga agatggcttc gacgatttcg taggttaacc
    14941 gtgaaaaacc aggttccccc tttgatgtaa taacgacgat attcagacat ggcgactcct
    15001 ttttctcaac ggtaagaaga aaaggaaagg tcggcaatga ggtgatggaa taacgggaaa
    15061 gcggctcgca taaacgcaca gatttgtagg catgataaga cgcgccagcg tcgcatcagg
    15121 catcggtgca caattgccga atccggcgtg aacgccctct ccgtcctaca aaatcgtctc
    15181 ttccgtcaaa atccgccgcg cgcctaaaaa atgatcctgc cagaaaggtt cagctaaccg
    15241 gcttatccga atggtttcgc cggtacgtgg tgactcgata aactgaccgt ctcccagata
    15301 cacccccata tgatcggcta tctcgcggct gtgaatatgg aaaaacagca aatctccccg
    15361 gcgcaggtcg ttgttcgcca caatcgttgc ccgatggtag tgatacatct cattggctgt
    15421 gcgcgggagc ttcgcctcaa ggatcttgtt gtaggcataa aaaaccaacc cgctacagtc
    15481 gaagccttta tcgggccgcg tgccgcccca gacataaggc ttgccaagct gctgctccag
    15541 gcggtgaatc gccacttcgg tgatgttgtg tagatggtcg ctgcttaaga aatggttgtt
    15601 ctccgccagc gcctgccagc gtctgtcact cgccttgagc ggcgcaggaa accattctgg
    15661 atgctgtttt atctgttcgc ggttgtgctg gcgcagcgcc tttctgcttt cggcattgcc
    15721 ttccacaata tagccggcct gctttttcag atgagtttgg tactgtttta ataaacgcgc
    15781 acggttttgc atccgctggc gggcggcgta gctgaaagag atatgtgatg tggtggcgct
    15841 ggcaaatgaa gaaaacggca agcaaagcaa caataatccc gacaacacaa aagagggaat
    15901 cgacggcaag gacatactca ataatggctg gtcatgcatt gatgttgcag tgtatcccgg
    15961 cccgtacctg aacaaactta ataaaccatt attataaaat taataaatat atttcagtta
    16021 attatgtaaa aacatccgtt tattaaatta ctcatggatt gtcgctgttg ctcatttgag
    16081 ctacaattca agctgaataa atatacagca caggagatat cccaatggct gctaacgcgt
    16141 ttgttcgcgc ccgaatcgat gaagatctga agaatcaggc ggcggacgta ctggccggga
    16201 tggggctgac catctctgac ctggttcgca taaccctcac aaaggtcgcg cgtgaaaagg
    16261 cattgccgtt tgatttacgc gagcctaatc aattaactat tcaatcaatc aaaaacagcg
    16321 aagctggcgt tggtattcat aaggccaaag acgccgatga tttatttgat aaattaggaa
    16381 tttaaatatg attcaaaggg atattgaata ctcgggacaa ttttcaaagg atgtaaaact
    16441 tgcacaaaag cgtcataagg atatgaataa attgaaatat cttatgacgc tcttatcaat
    16501 aatactttac tgcttccagc tgtttataaa gaccacccgc tgcaaggttc atggaaaggt
    16561 tatcgcgatg ctcatgtcga accggactgg atcctgattt acaaacttac cgataaactt
    16621 ttacgatttg agagaactgg aactcacgcg gcgctatttg ggtaaagccc gctgccgatg
    16681 aaatttgtaa accctgcgcc gacaggcttg cgtaaaactc ttttggtgat aatgtgagcc
    16741 ttccaaatcc agactattcc gatttgggat ccattttgcc gtcctggcgt gtgtaaccgt
    16801 tttatcaagg aataagcagt atgcgtaaaa tcgcattaat tcttgcgatg ttgttgatcc
    16861 cgtgcgtttc gtttgccggt ttgctgggca gcagtagctc gaccacgcca gtgagcaaag
    16921 agtataagca gcagttgatg ggatcccctg tctacatcca gatcttcaag gaagaacgta
    16981 cgctcgatct ctacgtcaaa atgggcgagc aatatcaact gctcgacagc tataaaatct
    17041 gtaaatattc cggcggctta gggccaaaac agcgtcaggg cgatttcaaa agcccggaag
    17101 ggttttatag cgtccagcgt aatcagttaa aaccagacag ccgttactac aaagcgatta
    17161 atattggatt cccaaacgcc tatgaccgtg cgcatggtta tgaagggaaa tacctgatga
    17221 ttcacggcga ttgtgtttcc atcggctgct acgcgatgac caatcagggt attgatgaga
    17281 tcttccagtt cgttactggt gcgctggtgt ttggtcagcc gagcgtgcaa gtgagtattt
    17341 acccgttccg tatgaccgac gccaatatga agcgccataa atattccaac tttaaggact
    17401 tctgggagca actgaagcca ggctacgact actttgagca aacccgtaag ccaccgaccg
    17461 tttctgtggt caatggccgt tacgtggtca gcaagccgtt aagccacgaa gttgtgcagc
    17521 cacaactggc atcaaactac acgctccccg aggcaaaata agcgccattc acctggcata
    17581 atcttttgcc aggtttcatt gcccgtcagc ggctgtgtcg caatcaccgt gaccacatca
    17641 ttcggtgtgg tctgcgagct gaagtcgatt tccacatcct gatccagcaa cgttgccacg
    17701 ccaaacggcg cgcggcgggt gatccagtgt aaattagtcg agcaatacgc cattacatag
    17761 cgcccgtcag aaagcagcat gttgaaaacg cccttctgcc gcagttcatc cgccagtgag
    17821 gcgatatatt taaacactgc cgccatgttg cccggtgtgc gcgggtaacg ctgcgttaat
    17881 ttatgcagga gccagcaaaa ggctttttcg ctatcggttt caccgaccgg gcggaagttg
    17941 ccggtttcca gtgatttgta gcccgtcagt tgtccattat gggcataagt ccagttacgc
    18001 ccccataact cgcgggtaaa cgggtgagta ttttccagcg ccacctcgcc ccgattagcc
    18061 tggcgaatat gagccaccac cgaacaggat tttatcgggt agtcctggac aagtttggcg
    18121 atgggggaat taaagctggg ttgcggatct ttaaatgtgc gacagccttt accttcgtaa
    18181 aaggtaatgc cccagccatc tttatgtggc ccggttccac caccacgctg tacaagtccg
    18241 gtgaaactaa agcagatatc ggtagggacg ttggcgctca tcccgagcaa ttcgcacatg
    18301 ggtaacctcc acaacagcgg gagcgcatgg cccccggcga agattgatta aaaagtacaa
    18361 aggttcaggc tgttaccctc accccggccc tctccctgaa agggagaaaa gaccttactt
    18421 aaccatctct ttttcaatta actggatcag gatatggatc actttaatgt ggatctcctg
    18481 aatgcggtcg gcataaccaa agtgcggtac gcgaatttcg atatccgctg tgccagccat
    18541 tttaccgccg tctttaccgg tcagggtgat cactttcatc cccttctcac gcgccgccgc
    18601 gatcgctttg atcacgtttg cagagttacc ggaggtggag atccccagca gtacatcgcc
    18661 ttcgcgacct accgcttcaa cgtaacggga gaaaatatca ttgaaaccaa aatcgttacc
    18721 gacgcaggaa atatgactaa cgtcagaaat ggcaatcgcc gggtagcccg gacggttttc
    18781 acggtagcga ccggtcaact cttcggcaaa gtgcatagcg tcgcaatggg agccgccgtt
    18841 gccacaggaa agcactttgc caccggcttt aaagctgtct gctaacagga ccgccgcgcg
    18901 ctgaatggcg tgaatattgg cgtcatcttt taaaaagtta gccagcgttt ccgccgcttc
    18961 gttcagttca ttacgaataa gatcctggta catgaggata tccttcagca taaatgtaat
    19021 agacaaaatg cagtgtaccg gataccgcca aaagcgagaa gtacgagcag gtgctatgac
    19081 caggactttt tgacctgaag tgcggataaa aacagcaaca atgtgagctt tgttgtaatt
    19141 atattgtaaa catattgcta aatgttttca catccactac aaccatatca tcacaagtgg
    19201 tcagacctcc tacaagtaag gggcttttcg ttatgatgat tttgagtatt ctcgctacgg
    19261 ttgtcctgct cggcgcgttg ttctatcacc gcgtgagctt atttatcagc agtctgattt
    19321 tgctcgcctg gacagccgcc ctcggcgtcg ctggtctgtg gtcggtgtgg gtactggtgc
    19381 ctctggccat tatcctcgtg ccgtttaact ttgcgcctat gcgtaggtcg atgatttccg
    19441 cgccggtatt tcgcggtttc cgtaaggtga tgccgccgat gtcgcgcacg gagaaagaag
    19501 cgattgatgc gggcaccacc tggtgggagg gcgacttgtt ccagggcaag ccggactgga
    19561 aaaagctgca taactatccg cagccgcgcc tgaccgctga agagcaagcg tttctcgacg
    19621 gtccggtaga agaagcctgc cggatggcga atgatttcca gattacccat gagctggcgg
    19681 atttgccgcc ggagctgtgg gcgtatctta aagatcatcg tttcttcgcg atgatcatca
    19741 aaaaagagta cggcgggctg gagttctcgg cctatgccca gtctcgcgtg ctgcaaaaac
    19801 tctccggcgt gagcgggatc ctggcgatta ccgtcggcgt gccaaactca ttaggcccgg
    19861 gtgagctact gcaacattac ggcactgacg agcagaaaga tcactatctg ccgcgtctgg
    19921 cgcgtggtca ggagatccct tgctttgcac tgaccagccc cgaagcgggt tccgatgcgg
    19981 gcgcgattcc ggataccggg attgtttgca tgggcgagtg gcagggccag caggtgctgg
    20041 ggatgcgact gacctggaac aaacgctaca ttacgctcgc accgattgcg accgtgcttg
    20101 gtctggcgtt taaactctcc gacccggaaa aactgctggg tggcgcagaa gatttaggta
    20161 ttacctgtgc gctgatcccg accaccacgc cgggcgtgga aattggtcgt cgccacttcc
    20221 cgctgaacgt accgttccag aacggaccga cgcgcggtaa agatgtcttc gtaccgatcg
    20281 attacatcat cggcggacca aaaatggccg ggcaaggctg gcggatgcta gtggagtgcc
    20341 tctcggtagg acgcggtatc accctgcctt ccaactcaac cggcggcgtg aaatcggtag
    20401 cgctggcaac gggtgcgtat gcgcacatcc gccgtcagtt caaaatctct attggtaaga
    20461 tggaagggat tgaagagccg ctggcgcgta ttgccggaaa cgcttatgta atggatgctg
    20521 cggcatcgct gattacatac ggcattatgc tcggtgaaaa acctgccgtg ctgtcggcta
    20581 tcgttaagta tcactgtacc caccgtgggc agcagtcgat tattgatgcg atggatatca
    20641 ctggtggcaa aggcattatg ctcgggcaaa gcaacttcct ggcccgtgct taccagggcg
    20701 caccgattgc catcaccgtt gaaggggcta acattctgac ccgcagcatg atgatcttcg
    20761 gacaaggagc gattcgttgc catccgtacg tgctggaaga gatggaagcg gcgaagaaca
    20821 atgacgtaaa tgcgttcgat aaactgctgt tcaaacatat cggtcacgtc ggtagcaaca
    20881 aagttcgcag cttctggctg ggcctgacgc gcggtttaac cagcagcacg ccaaccggcg
    20941 atgccactaa acgctactat cagcacctga accgcctgag cgccaacctc gccctgcttt
    21001 ctgatgtctc gatggcggtg ctgggcggca gcctgaaacg tcgcgagcgc atctcggcac
    21061 gtctggggga tattttaagc cagctttacc tcgcctctgc cgttctgaag cgttatgacg
    21121 acgaaggccg taatgaagcc gacctgccgc tggtgcactg gggcgtacag gatgcgctgt
    21181 atcaggcaga gcaggcgatg gacgatttac tgcaaaactt cccgaaccgc gtggttgccg
    21241 ggctgctgaa tgtggtgatc ttcccgaccg gacgtcatta tctggcaccg tctgacaagc
    21301 tggatcataa ggtggcgaag attttacaag tgccgaacgc cacccgttca cgtattggtc
    21361 gcggtcagta cctgaccccg agcgagcata acccggttgg tttactggaa gaggcgctgg
    21421 tggatgtgat tgccgccgac ccaattcatc agcggatctg taaagagctg ggtaaaaacc
    21481 tgccgtttac ccgtctggat gaactggcgc ataacgcgct ggcgaagggg ctgattgata
    21541 aagatgaagc agctattctt gtgaaagctg aagaaagccg tctgcgcagt attaatgttg
    21601 atgactttga tccggaagag cttgcgacga agccggtaaa gttgccggag aaagtgcgaa
    21661 aagttgaagc cgcgtaattg aataacggag ccgaaaggct ccgtttcttt atccgctaat
    21721 tatttaaaat taaagccatc cggatggttt tccaggctgc cggtcaacgc cgcgaacagc
    21781 accgttttac catcaataga aagcgcatcg ttcacattca accaggtgag tttctcttgc
    21841 gacgttttct catcaatggt cgagaacagc cccgtcatct gattagattt ctcggaccac
    21901 atcacagcga tacgttgcga gccacagtca tgcggtttgc acgcgctcat cacctgatac
    21961 gtctcatctc ccaacgttac ggtttgtgca ggggtataag taccgccttt catcacccag
    22021 gcaggcagct tatgcccttg caccatctga ttaaacgccg ctttggtggt ttcgcccttt
    22081 gcaaggctgc taatggttaa atcatcctgc gccattgcac tggtggcgat gaccagagcg
    22141 gcgactgtcg ttattgcctt aaacatcatt cctcccgagc ttatcctgcc catgttatta
    22201 acctcctaaa gcctggcgat aacacgataa tttgcaaatc agatatttcc aatatcagca
    22261 tttgttatca atttattctg cgctaaggca actgcctgat aaggtgttag agttataaaa
    22321 cccttcgcat aaaggagttg tacaccgtgc ctggtttgaa gattacgctt ttgcagcaac
    22381 cactggtgtg gatggatggc cctgccaacc tgcgtcattt tgatcgtcaa ctggaaggta
    22441 ttaccggacg cgatgtgatc gttctaccgg agatgtttac cagcggcttt gccatggaag
    22501 cggcagctgc gtcgctagca caagatgacg tagtgaactg gatgacagcc aaagcgcagc
    22561 agtgcaatgc gctgattgca ggcagtgttg cattacaaac ggagtctggt tcggttaacc
    22621 gctttttgct ggttgagccg ggcggcacgg tacattttta tgataagcgt catctgttcc
    22681 gaatggcaga tgagcatctg cattataaag cgggcaatgc gcgagtaatt gtggaatggc
    22741 gcggctggcg tattttgccg ctggtgtgct acgacttacg ttttcctgtg tggtcgcgca
    22801 atctcaacga ttatgacctc gccctgtacg tcgccaactg gcctgctccg cgctctctgc
    22861 actggcaggc attgctgaca gcccgcgcga ttgagaatca ggcgtatgtg gcgggatgca
    22921 atcgcgtcgg cagcgatggc aacggctgcc attatcgcgg tgacagcaga gtgattaatc
    22981 cacagggcga gattatcgcc accgccgacg cgcatcaggc aacgcgcatt gatgcggagc
    23041 tgtcgatggt ggcgttgcgg gaatatagag aaaagtttcc ggcatggcgg gatgcggatg
    23101 aattcacatt tcgttagagg gtgaatatgg ctacgagata agtttcgtgg ctatttttct
    23161 ctggaactta aacgacgaaa gcccgaatca gtaatgattc gggctttctg aatattggcg
    23221 gaacggacgg gactcgaacc cgcgaccccc tgcgtgacag gcaggtattc taaccgactg
    23281 aactaccgct ccgcgttgtg ttccgttggg aacgaggcga atattacgga ttgcctcacc
    23341 cttcgtcaac ggtttttctc atcttttgaa tcgtttgctg caaaaatcgc ccaagtcgct
    23401 atttttagcg ccttttacag gtatttatgc ccgccagagg cagcttccgc ctttcttctg
    23461 caccagatcg agacgggctt catgtgccgc aagctcttca tctgtcgcaa aaacaacgcg
    23521 taacttactt gcctgacgca caatgcgctg aattgttgct tcaccttgtt gctgttgtgt
    23581 ctctccttcc atcgcaaaag ccatcgacgt ttgaccaccg gtcatcgcca gataaacttc
    23641 cgcaaggatc tgggcatcga gtaatgcccc gtgcagcgtt cgtttactgt tatctatttc
    23701 gtagcgggca cataacgcat cgaggctgtt gcgcttaccg ggaaacattt tcctcgccac
    23761 cgcaaggcta tcggtgacct tacagaaggt attggtcttc ggaatatcgc gcttaagcaa
    23821 cgaaaactcg tagtccataa agccgatatc gaacgctgcg ttatggatca cgagctccgc
    23881 gccgcggata tagtccatga actcatcggc aacctcggca aacgtgggct tatcgagcaa
    23941 aaactcatcg gcaataccat gtacgccaaa ggcttccgga tccaccagcc gatcgggttt
    24001 gagataaaca tggaagttat tgcccgtcag gcgacggttc accacttcaa cggcaccaat
    24061 ctcaatgatc ttgtggcctt catagtgcgc accaatctga ttcataccgg tagtttcggt
    24121 atcgagaacg atctggcgtg taattgcagt gctcatagcg gtcatttatg tcagacttgt
    24181 cgttttacag ttcgattcaa ttacaggaag tctaccagag atgcttaaac aggtagaaat
    24241 tttcaccgac ggttcgtgtc tgggtaatcc aggaccgggg ggatacggtg ctattttacg
    24301 ctatcgcgga cgcgagaaaa cctttagcgc aggctacacc cgcaccacca acaaccgtat
    24361 ggagttgatg gccgctattg tcgcgctgga ggcgttaaaa gaacattgcg aagttatttt
    24421 gagtaccgac agccagtatg tccgccaggg tatcacccag tggatccata actggaaaaa
    24481 acgtggctgg aaaacagcag acaaaaaacc agtaaaaaat gtcgatctct ggcaacgtct
    24541 tgatgctgcg ctggggcagc atcaaatcaa atgggaatgg gttaaaggcc atgccggtca
    24601 tccagaaaac gaacgttgcg atgaactggc tcgtgccgca gcgatgaatc ccacactgga
    24661 agatacgggc tatcaagtcg aagtttaagc ctgtggttta cgacattgcc gggtggctcc
    24721 aaccgcctgg cgaattcgtg gcttgttttt actctgtttc atcggattta gcgttaaagg
    24781 aatagtccgt ttccgggcga caataagttg taagcaacca agcgcaggaa tatgcgcatt
    24841 caatagtttt ccaccgtgtt tgttccacgg gagaacgtgg aaacggctgg cgtgtagcac
    24901 ttcaaaattc aacaaagaga gccagtccag ttgccgcatc agagtaaaca tccggctatt
    24961 atagggcgag gttttgcgca ataccggcac aagtttgcgt aatcccatta aactgatggg
    25021 gttgaagcca ctaatcacca gccagccatc atcaatcagt acccgatcgg cttcacgcaa
    25081 taaacgatgc ggatcggtgc accacggcaa ggtatgtgcc agtagacaaa catcaacgga
    25141 tttatcggca aaaggaagat gaagtggatc cgcctgcacc tggacgggca ttccttgcgc
    25201 agaaacattc acttgatgag aaaccgcgca cgcttcgcaa ttgatttctg cgcttaaatt
    25261 gccaatctta agcagatgaa aaccatacat tttagtgaac cacgggttga gctggcgctc
    25321 cagcgccttg cgataaagct ctccccaggg caaatcgccc cagcaatcag gagccacgac
    25381 agtttgaggg actcttgccg gtttcatcac aaccttccgt ttcacactga gaggtaatct
    25441 atgaatctta acagtattcc cgcctttgat gacaattaca tctgggtttt gaatgatgaa
    25501 gcaggtcgct gcctgattgt cgatcccgga gacgcagagc cagtattaaa cgccattgcc
    25561 gccaataact ggcaaccgga ggccatattt ctcacccacc atcaccacga tcacgttggc
    25621 ggcgtaaaag aactggtgaa aaagtttccg caaattgtgg tttatggtcc acaagagaca
    25681 caagataagg gaacaacaca ggtagtcaaa gatggcgaaa ctgccttcgt tttggggcat
    25741 gaatttagtg taattgccac gccgggtcac actttaggac atatctgtta cttcagtaaa
    25801 ccttatctat tttgcggcga cacgctgttt tctggtgggt gtggtcggtt gtttgaaggg
    25861 acagcatcac aaatgtatca atcacttaaa aagttaagtg cgttacctga cgatacattg
    25921 gtatgttgtg ctcatgaata tacgttatca aatatgaagt ttgctttgag tattcttccg
    25981 cacgatttgt ccataaatga ttattatcgt aaagttaagg agttacgggc aaaaaatcaa
    26041 ataacactac ccgtaattct gaaaaatgag cggcaaatta atgttttttt aagaacggaa
    26101 gatattgatt taattaatgt aattaatgaa gaaacattat tgcaacaacc tgaagaacgt
    26161 tttgcttggt taaggtcaaa gaaagatagg ttctgataaa actttcttgt catcggctcc
    26221 gttcgccgtt atgatcggtc gtcttttaag caactattga cacacacatg aaggcaaaag
    26281 cgatattact cgcctctgtc ctgctcgtgg gttgccagag caccggcaac gttcaacagc
    26341 acgcacagag cctttctgca gctggtcaag gggaagcagc aaagtttaca agtcaggcac
    26401 gatggatgga cgatgggacg tctatcgcgc cagatggtga cttgtgggct ttcattggcg
    26461 acgagctaaa gatgggaatt ccggaaaacg accggattcg cgaacagaaa cagaaatatt
    26521 tacgcaataa gagctatctc cacgatgtaa ctttacgggc agagccgtat atgtactgga
    26581 tagccgggca agttaaaaaa cgtaacatgc ctatggaact ggtactacta cccatagtgg
    26641 agagcgcttt tgatcctcac gcaacgtctg gcgccaatgc cgcgggcatc tggcagatca
    26701 ttccgagcac ggggcgcaat tatggtttga aacagacccg caattatgac gcgcgtcgcg
    26761 atgttgttgc ttcaacaact gccgcgctga acatgatgca gcgtctgaac aagatgtttg
    26821 acggcgactg gcttctgacc gtagcggctt ataacagcgg cgaaggtcgg gtcatgaagg
    26881 caattaaaac gaacaaagcg cgtgggaaat ccacggactt ctggtcgtta ccgttgccgc
    26941 aggaaacgaa gcagtacgtg cctaaaatgc tggcattgag tgatattctc aaaaacagca
    27001 agcgttatgg cgtacgtctg ccaacgaccg atgaaagccg tgctctggcg cgtgtgcacc
    27061 tgagcagccc ggttgaaatg gcgaaggttg cagatatggc ggggatttcc gtcagcaagc
    27121 tgaagacatt caacgctggc gtgaaaggct ccacgctggg cgcaagtggt ccgcagtacg
    27181 tgatggtgcc aaagaagcat gcagatcaac tgcgtgaatc tctggcttca ggcgaaattg
    27241 ctgctgtaca gtcgacgctg gttgccgaca atacgccgct taacagccgt gtttacaccg
    27301 tacgctctgg cgacacgctt tcaagtatcg cttcacgtct cggcgtaagc accaaagatt
    27361 tgcagcagtg gaacaaactg cgcggatcta agctgaagcc aggccaaagt ttgacgattg
    27421 gtgcaggcag tagcgcacag cgactggcaa acaacagcga tagcattacg tatcgtgtgc
    27481 gcaaaggcga ttcgctttca agcattgcta aacgccacgg cgtgaacatc aaagatgtaa
    27541 tgcgctggaa cagcgatact gcgaatctgc aaccaggcga taagctgacg ttgtttgtga
    27601 aaaacaacag catgccagac tcctgacaaa ccagataata aaaaaggcac cgattccccc
    27661 ggtgcctttt ttatttatgc cgctttatgt gcttctaaca tgatgatatc actggtgaaa
    27721 gagccatcat tctgcaaggc aaaatacgtt ttcacctctg tcgatgcgct ctgttggtaa
    27781 atgcgaatag cgtctactaa cgcttctggc gtacgcattc tcgctaccca tgaagaaaat
    27841 tccagcggta acttatctgt aattaaatta tcaactatca gattggcttc attgattaac
    27901 ttcaaccact caccgctggc gtagtttcgt acgtgagagg tatcgcgtaa tgcttctacc
    27961 gtctgtaacc agatgtcgcg cactggatga cccggagaca ttacgtccat cacaatcagc
    28021 ctaccgccgg gttccaatac ccgattcact tcacgcagtg ctgcaccaac atcatgccag
    28081 tgatgggcag aataacggct gataacaata tcaaatgcgt tatcggcaaa tggcagactt
    28141 tcggcataac cctggcgggt ggtgatattt ttcagttgcc gggcttcggc agcttgtgcc
    28201 acgacatcca gcatttgggc agataagtca tacgccacca ccgcgctcac gttctgcgca
    28261 gcgacaaagc tggcatgccc tgctccgcag cccatatcaa gtacacttgc atcaggataa
    28321 tcagccagac gcaccgccag gcgctgtaaa tctcgcccgg atgcatgtac ggtactggtt
    28381 aaatactcac tggcctgcga gctaaactgc ttttctacgt ggtcatggtg ggattgtgtt
    28441 gtcattctgt ccttttattg tttgattaaa aagggcagcg ccaaacgttg gtctgccctg
    28501 tggcagacct gacatacctt atttatcagg cttgccggga ctgaattcaa cgagtagcgg
    28561 attgtgatcg gaagcgcgcg taaccagtac agaagcttcg ctgacgttca gaccacggta
    28621 gaaaacaaaa tcgagcgggc gaccaaacgc ccgacggcgc tgatcatcgg taaaacgcac
    28681 ctggcgcagc gacatttccc gcgcaaagcg atataacgcg ttcatccttc tacggctcca
    28741 ggcattgaaa tctcccgcca taatgaccgg gccgctgtgg tgagctatct gatcgccaat
    28801 aggaagtaac tgcttactat agacatccac gcccaaactg aaattgacgg cgtgtatatt
    28861 aaccaccatc aacaggcggg tgtcaggcaa tggatagacc gtcaccagtg ccgacttcgc
    28921 cagacgcaaa atgggttctc gttcacgtaa cgggcagcaa tacactggat gtgccgccga
    28981 aagggtcatt acgccagaag gatgttgtgg cagcacgaaa gcgggtacct gatcggcggc
    29041 aagatagtta gcagtcgcaa actgtactaa ctctggcgtt gtctgcgctt cctgcaataa
    29101 caccagatgt gcatctttgc cgtagttctt taataccgac aaccattcag cgcgttgctg
    29161 tttgtatatg ttccacacca ggatccgaat acgctcttcg gtacttaacg gttctccagg
    29221 tggtaatgcc tggccgatgc tcgcaaaaga ccccggcggt aagatccttt ccgcaggttg
    29281 tccggcaaca tagcgcatgg cataggtgtt ttttcgcact tttgactttg aaacctctat
    29341 tacgtcaact cttccgcact cgggagggtt ctactgttat agggatttta gctcactctt
    29401 tcaacgagca attgtatatt gctatgtaag caagtgctta caccggtcag aaatttgctc
    29461 attattgaga acattaaatc ggatgacata tctcgcatcc tgatggttta atgacaatta
    29521 aattcaaaat atctcttcta ttttgcaaca ggagcaaaaa tgaaagccac gtcggaagaa
    29581 ctcgccattt ttgtttcagt cgtcgaaagc ggcagcttta gccgggcagc ggaacaatta
    29641 gggcaagcaa actcagcggt aagccgggcg gtgaaaaagc tggagatgaa acttggcgtt
    29701 agcctgctta atcggaccac gcggcaactt agcctgacgg aagaaggcga gcgttatttt
    29761 cgtcgcgtac agtcaatttt gcaggagatg gcagcggcag aatcagaaat tatggagacg
    29821 cgtaatacac cgcgtggact gttacggatc gatgccgcaa ctccagtggt gctgcacttt
    29881 ctgatgccgt tgattaagcc ttttcgtgaa cgctatccgg aagtcacttt atcgctagtc
    29941 tcctccgaaa cgattattaa tttgatcgaa agaaaagtgg atgtcgcgat acgcgccggt
    30001 acgttaacgg attccagctt acgtgccagg ccgttattta acagttatcg aaaaattatc
    30061 gcctcccccg attatatttc ccgctacggg aagccagaaa cgatcgacga tttaaagcaa
    30121 catatttgcc tgggattcac tgaacccgct tccctcaata cctggccgat agcctgtagc
    30181 gatggacaat tacatgaggt gaagtacggt ttgtcatcca atagtgggga aacactgaaa
    30241 cagctttgcc tgagtggcaa cgggattgcg tgtttgtccg actacatgat cgacagagaa
    30301 atcgctcgcg gagaattggt ggagttaatg gcagataaag tattgccagt ggaaatgcca
    30361 ttcagtgccg tatattacag cgaccgtgcg gtaagtacac gcatccgggc ttttatcgat
    30421 ttccttagcg agcatgtaaa aacagctccc ggaggagctg tcagagaggc ttaatcccat
    30481 tcaggagcca gaccttccgg gctaaccagg cggtcgttgc aatccagtgc ggcgatcgct
    30541 tttttatctt cggcatcaag ctgtaaattt tgtgccttaa gattactttc caggttttta
    30601 cgtttagtag aagaaggaat tacggagtaa ccttccccca tagcccacgc cagaatcact
    30661 tgtgccggag tcgcattgtg tttagctgcg atacgagcaa taacctcatc tttcagggcc
    30721 ttaccatacg ccagcgtcat ataggaagta atatggatgc cgtgctgttt agcccaggcg
    30781 accactttac ggttttgcag ataaggtgag agttcaatct ggttagtagc gatgttttca
    30841 gcgccaacag cagcaatcgc cttttccatc aatgggatcg tgaagttgga aataccgatc
    30901 tcacgcgtca gcccttgttt tttggcttcc agcagcgcct gcataaactc ttcaacagag
    30961 acttcatcgt ttggtgacgg ccagtggatt agcgtcagat caacataatc gatacgcaat
    31021 ttttgcaggc tctctttcag acttggggtc aatttgtctt tgctgagatt ttcaatccag
    31081 attttagtgg tgatgtagag ttcattacgt ggcacgccac tttctgcaat cgcctgacct
    31141 actgcggctt cgttatcata gatttgtgcg gtatcaattg cgcgataacc aagttcaagc
    31201 gccgttttca cagatgaaat aacaacgtcg tctttcagac ggaaagtacc taaaccaaat
    31261 gcagggatag ccataatatg cctcttttaa tgctattttc gttaactgaa gtgattatgg
    31321 gctttagaat gttgcggaaa aagggcgtaa aacgcagagg atttttgcga tttaagcaat
    31381 aatagaaata tagacgaaaa aaaacctcga gcgttaactc gaggcttctt aata
//
LOCUS       DAMZFT010000050        31204 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.050, whole genome shotgun sequence.
ACCESSION   DAMZFT010000050 DAMZFT010000000
VERSION     DAMZFT010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 31204)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31204)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31204
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.050"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..606
                     /locus_tag="PN977_002361"
     CDS             <1..606
                     /locus_tag="PN977_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDI6042387.1"
                     /translation="LQEREFERIGGHQTIKVDIRIIAATNRDLQAMVKEGTFREDLFY
                     RLNVIHLILPPLRDRREDISLLANHFLQKFSSENQRDIIDIDPMAMSLLTAWSWPGNI
                     RELSNVIERAVVMNSGPIIFSEDLPPQIRQPVCNAGEAKTAPVGERNLKEEIKRVEKR
                     IIMEVLEQQEGNRTRTALMLGISRRALMYKLQEYGIDPADV"
     gene            802..1464
                     /gene="atoD"
                     /locus_tag="PN977_002362"
     CDS             802..1464
                     /gene="atoD"
                     /locus_tag="PN977_002362"
                     /EC_number="2.8.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate CoA-transferase subunit alpha"
                     /protein_id="HDI6042388.1"
                     /translation="MKTKLMTLQDATGFFRDGMTIMVGGFMGIGTPSRLVEVLLESGV
                     RDLTLIANDTAFVDTGIGPLIVNGRVRKVIASHIGTNPETGRRMISGEMDVVLVPQGT
                     LIEQIRCGGAGLGGFLTPTGVGTVVEEGKQTLTLDGKTWLLERPLRADLALIRAHRCD
                     TLGNLTYHLSARNFNPLIALAADITLVEPDELVETGELQPDHIVTPGAVIDHIIVSQE
                     SK"
     gene            1464..2114
                     /gene="atoA"
                     /locus_tag="PN977_002363"
     CDS             1464..2114
                     /gene="atoA"
                     /locus_tag="PN977_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate CoA-transferase subunit beta"
                     /protein_id="HDI6042389.1"
                     /translation="MDAKQRIARRVAQELRDGDIVNLGIGLPTMVANYLPEGIHITLQ
                     SENGFLGLGPVTTAHPDLVNAGGQPCGVLPGAAMFDSAMSFALIRGGHIDACVLGGLQ
                     VDEEANLANWVVPGKMVPGMGGAMDLVTGSRKVIIAMEHCAKDGSAKILRRCTMPLTA
                     QHAVHMLVTELAVFRFIDGKMWLTEIADGCDLATVRAKTEARFEVAADLNTQRGDL"
     gene            2111..3433
                     /locus_tag="PN977_002364"
     CDS             2111..3433
                     /locus_tag="PN977_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00366 family protein"
                     /protein_id="HDI6042390.1"
                     /translation="MIGRISRFMTRFVSRWLPDPLIFAMLLTLLTFVIALWLTPQTPI
                     SMVKMWGDGFWNLLAFGMQMALIIVTGHALASSAPVKSLLRTAASAAKTPVQGVMLVT
                     FFGSVACVINWGFGLVVGAMFAREVARRVPGSDYPLLIACAYIGFLTWGGGFSGSMPL
                     LAATPGNPVEHIAGLIPVGDTLFSGFNIFITVALIVVMPFITRMMMPKPSDVVSIDPK
                     LLMEEADFQKQLPKDAPPSERLEESRILTLIIGALGIAYLAMYFSEHGFNITINTVNL
                     MFMIAGLLLHKTPMAYMRAISAAARSTAGILVQFPFYAGIQLMMEHSGLGGLITEFFI
                     NVANKDTFPVMTFFSSALINFAVPSGGGHWVIQGPFVIPAAQALGADLGKSVMAIAYG
                     EQWMNMAQPFWALPALAIAGLGVRDIMGYCITALLFSGVIFVIGLTLF"
     gene            3464..4648
                     /gene="atoB"
                     /locus_tag="PN977_002365"
     CDS             3464..4648
                     /gene="atoB"
                     /locus_tag="PN977_002365"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA acetyltransferase"
                     /protein_id="HDI6042391.1"
                     /translation="MKNCVIVSAVRTAIGSFNGSLASTSAIDLGATVIKAAIERAKID
                     SLHIDEVIMGNVLQAGLGQNPARQALLKSGLAETVCGFTVNKVCGSGLKSVALAAQAI
                     QAGQAQSIVAGGMENMSLAPYLLDAKARSGYRLGDGQVYDVILRDGLMCATHGYHMGI
                     TAENVAKEYGITREMQDELALHSQRKAAAAIESGAFTAEIVPVNIVTRKKTFVFSQDE
                     FPKANSTAEALGALRPAFDKAGTVTAGNASGINDGAAALVIMEESAALAAGLTPLARI
                     KSYASGGVPPALMGMGPVPATQKALQLAGLQLADIDLIEANEAFAAQFLAVGKTLGFD
                     SEKVNVNGGAIALGHPIGASGARILVTLLHAMQARDKTLGLATLCIGGGQGIAMVIER
                     LN"
     gene            complement(4722..5498)
                     /locus_tag="PN977_002366"
     CDS             complement(4722..5498)
                     /locus_tag="PN977_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaP family protein"
                     /protein_id="HDI6042392.1"
                     /translation="MRKIFLPLLLVALSPVAHSEGVQEVEIDAPLSGWHPAEGEDASF
                     SQSINYPASSVNMADDQNISAQIRGKIKNYAAAGKVQQGRLVVNGASMPQRIESDGSF
                     ARPYIFTEGSNSVQVISPDGQSRQKMQFYSTPGAGVIRARLRLVLSWDTDDTDLDLHV
                     VTPDGEHAWYGNTVLNNSGALDMDVTTGYGPEIFAMPAPVHGRYQVYINYYGGRSETE
                     LTTAQLTLITDEGSVNEKQETFIVPMRNAGELTLVKSFDW"
     gene            complement(5503..7152)
                     /locus_tag="PN977_002367"
     CDS             complement(5503..7152)
                     /locus_tag="PN977_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaQ family protein"
                     /protein_id="HDI6042393.1"
                     /translation="MNWRRIVWLLALVTLPTLAEETSLQLALRGAQHDQLYQLSSSGV
                     TKVSALPDTLTTPLGSLWKLYVYAWLEDTHQPEQPYQCRGNSPEEVYCCQAGESITRD
                     TALVRSCGLYFAPQRLHIGADVWGQYWQQRQAPAWLASLTTLKPETSVTVKSLLDSLA
                     TLPAQNKAQEVLLDVVLDEAKIGVASMLGSRVRVKTWSWFADDKQEIRQGGFAGWLTD
                     GTPLWVTGSGTSKTVLTRYATVLNRVLPVPTQVASGQCVLVELFARYPLKKIAAEKST
                     TAVKPGVLNGRYRVTFANGNHITFVSHGETTLLSEKGKLKLQSHLDREEYVARVLDRE
                     AKSTPPEAAKAMTVAIRTFLQQNANREGDCLTISDSSATQRVSASPATTGARTMTAWT
                     QDLIYAGDPVHYHGSRATEGTLSWRQATAQAGQGERYDQILAFAYPDNSLSRWGAPRS
                     TCQLLPKAKAWLAKKMPQWRRILQGETGYNEPDVFAVCRLVSGFPYTDRQQKRLFIRN
                     FFTLQDRLDLTHEYLHLAFDGYPTGLDENYIETLTRQLLMD"
     gene            complement(7153..11667)
                     /locus_tag="PN977_002368"
     CDS             complement(7153..11667)
                     /locus_tag="PN977_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000536590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
                     /protein_id="HDI6042394.1"
                     /translation="MGTGLANADDSLPSSNYAPPGGGTFFLLADSSFSSSEEAKVRLE
                     APGRDYRRYQMEEYGGVDVRLYRIPDPMAFLRQQKNLHRIVVQPKYLGDGLNNTLTWL
                     WDNWYGKSRRVMQRTFSSQSRQNVTQALPELQLGNAIVKPSRYVQNSQFSPLKKYPLV
                     EQFRYPLWQAKPVEPQQGVKLEGASSNFISPQPGNIYIPLGKQEPGLYLVEAMVGGYR
                     ATTVVFVSDTVALSKVSGNELLVWTAGKKQGEAKPGSEILWTDGLGVMTRGVTDDSGT
                     LQLQHISPERSYILGKDAEGGVFVSENFFYESEIYNTRLYIFTDRPLYRAGDRVDVKV
                     MGREFHDPLHSSPIVSAPAKLSVLDANGSLLQTINVTLDARNGGQGSFRLPENAVAGG
                     YELRLAYRNQVYSSSFRVANYIKPHFEIGLALDKKEFKTGEAVSGKLQLLYPDGEPVK
                     DARVQLSLRAQQLSMVGNDLRYAGRFPVSLEGSETVSDDSGHVALNLPAADKPSRYLL
                     TVSASDGAAYRVTTTKEILIERGLAHYSLSTAAQYSNSGESVVFRYAALESSKQVPVT
                     YEWLRLEDRTSHSGELPSGGQSFTVNFAKPGNYNLTLRDKDGLILAGLSHAVSGKGST
                     AHTGTVDIVADKTLYQPGETAKMLITFPEPIDEALLTLERDRVEQQSLLSHPANWLTL
                     QRLNDTQYEARVPVSNSFAPNITFSVLYTRNGQYSFQNAGIKVAVPQLDIRVKTDKTH
                     YQPGELVNVELTSSLKGKPVSAQLTVGVVDEMIYALQPEIAPNIGKFFYPLGRNNVRT
                     SSSLSFISYDQALSSEPVAPGATNRSERRVKMLERPRREEVDTAAWMPSLTTDKQGKA
                     YFTFLMPDSLTRWRITARGMNGDGLVGQGRAYLRSEKNLYMKWSMPTVYRVGDKPAAG
                     LFIFSQQDNEPVALVTKFAGAEMRQTLTLHKGANYISLTQNIQQSGLLSAELQQNGQV
                     QDSISTKLSFVDNSWPVEQQKNVMLGGGDNALMLPEQASNIRLQSSETPQEIFRNNLD
                     ALVDEPWGGVINTGSRLIPISLAWRSLADHQSAAANDIRQMIQDNRLRLMQLAGPGAR
                     FTWWGEDGNGDAFLTAWAWYADWQASQALGVTQQPEYWQHMLDSYAEQADNMPLLHRA
                     LVLAWAQEMNLPCKTLLKGLDEAIARRGTKTKDFSEADTSDINDSLILDTPESPLADA
                     VANVLTMTLLKKAQLKSTVMPQVQQYAWDKAANSNQPLAHTVVLLNSGGDATQAAAIL
                     SGLTAEQSTIERALAMNWLAKYMATMPPVVLPAPAGAWAKHKLTGGGEYWRWVGQGVP
                     DILSFGDELSPQNVQVRWREPAKTAQQSNIPVTVERQLYRLIPGEEEMSFTLQPVTSN
                     EIDSDALYLDEITLTSEQDAVLRYGQVEVPLPPGADVERTTWGISVNKPNAGKQQGQL
                     LEKARNEMGELAYMVPVKELTGTVTFRHLLRFSQKGQFVLPPARYVRSYAPAQQSVAA
                     GSEWTGMQVK"
     gene            complement(11691..12314)
                     /locus_tag="PN977_002369"
     CDS             complement(11691..12314)
                     /locus_tag="PN977_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311139.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1175 domain-containing protein"
                     /protein_id="HDI6042395.1"
                     /translation="MRHGLLALICWLCCVVAHSEMLNVEQSGLFRAWFVRIAQEQLRQ
                     GPSPRWYQQDCAGLVRFSANEALKIHDSKWLKSNGIASQYLPPEMTLTPEQRQLAQNW
                     NHGNGKTGPYVTAINLIQYNSQFIGQDINQALPGDMIFFDQGDAQHLMVWMGRYVIYH
                     TGSATKTDNGMRAVSLQQLMTWKDTRWIPNDSNPNFIGIYRLNFLAR"
     gene            complement(12311..13999)
                     /locus_tag="PN977_002370"
     CDS             complement(12311..13999)
                     /locus_tag="PN977_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416733.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2138 domain-containing protein"
                     /protein_id="HDI6042396.1"
                     /translation="MSGEKKAKGWRFYGLVGLGAIVLLSAGVWALQYAGSGPEKTLSP
                     LVVHNNLQIDLNEPDLFLDSDSLSQLPKDLLTIPFLHDVLSEDFVFYYQNHADRLGIE
                     GSIRRIVYEHDLTLKDKLFSSLLDQPAQAALWHDKQGHLSHYMVLIQRSGLSKLLEPL
                     LFAATSDSQLSKTEISSIKLNSETIPVYQLRYNGNNALMFATYQDKMLVFSSTDMLFK
                     DDQQDTEATAIASDLLSGKKRWQASFGLEERAAEKTPVRQRIVVSARLLGFGYQRLMP
                     SFAGVRFEMSNDSWHSFVALNDESASVDASFDFTPVWNSMPAGASFCVAVPYSHGIAE
                     EMLSHISQENDKLNGALDGAAGLCWYEDSKLQTPLFVGQFDGSAEQVQLPGKLFTQNI
                     GAHESKAPEGVLPVNQTQQGEAQIWRREVSSRYGQYPKAQAAQPDQLMSDYFFRVSLA
                     MQNKTLLFSLDDTLVNNALQTLNKTRPAMVDVIPTEGIVPLYINPQGVAKLLRNETLT
                     SLPKNLEPVFYNAAQTLLMPKLDALSQQPRYVMKLAQMEPGAAWQWLPITWQPL"
     gene            complement(14148..16775)
                     /gene="gyrA"
                     /locus_tag="PN977_002371"
     CDS             complement(14148..16775)
                     /gene="gyrA"
                     /locus_tag="PN977_002371"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit A"
                     /protein_id="HDI6042397.1"
                     /translation="MSDLAREITPVNIEEELKSSYLDYAMSVIVGRALPDVRDGLKPV
                     HRRVLYAMNVLGNDWNKAYKKSARVVGDVIGKYHPHGDLAVYNTIVRMAQPFSLRYML
                     VDGQGNFGSIDGDSAAAMRYTEIRLAKIAHELMADLEKETVDFVDNYDGTEKIPDVMP
                     TKIPNLLVNGSSGIAVGMATNIPPHNLTEVINGCLAYIDDEDISIEGLMEHIPGPDFP
                     TAAIINGRRGIEEAYRTGRGKVYIRARAEVEVDAKTGRETIIVHEIPYQVNKARLIEK
                     IAELVKEKRVEGISALRDESDKDGMRIVIEVKRDAVGEVVLNNLYSQTQLQVSFGINM
                     VALHHGQPKIMNLKDIIAAFVRHRREVVTRRTIFELRKARDRAHILEALAVALANIDP
                     IIELIRHAPTPAEAKTALVANPWQLGNVAAMLERAGDDAARPEWLEPEFGVRDGLYYL
                     TEQQAQAILDLRLQKLTGLEHEKLLDEYKELLDQIAELLRILGSADRLMEVIREELEL
                     VREQFGDKRRTEITANSADINLEDLITQEDVVVTLSHQGYVKYQPLSEYEAQRRGGKG
                     KSAARIKEEDFIDRLLVANTHDHILCFSSRGRVYSMKVYQLPEATRGARGRPIVNLLP
                     LEQDERITAILPVTEFEEGVKVFMATANGTVKKTVLTEFNRLRTAGKVAIKLVDGDEL
                     IGVDLTSGEDEVMLFSAEGKVVRFKESSVRAMGCNTTGVRGIRLGEGDKVVSLIVPRG
                     DGAILTATQNGYGKRTAVAEYPTKSRATKGVISIKVTERNGLVVGAVQVDDCDQIMMI
                     TDAGTLVRTRVSEISIVGRNTQGVILIRTAEDENVVGLQRVAEPVDEEDLDTIDGSAA
                     EGDDEIAPEVDVDDEPEEE"
     gene            complement(17275..18477)
                     /locus_tag="PN977_002372"
     CDS             complement(17275..18477)
                     /locus_tag="PN977_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HDI6042398.1"
                     /translation="MKITSVEIFDCELKKRDQTMASYNPVLIRVNTDAGISGVGEVGL
                     AYGAGAKSGVGIIRDLAPLIIGEDPLNIEKIWEFLFRKTFWGMGGGNVFYAGMSAIDI
                     ALWDIKGKYLNVPVYQLLGGKTNDKLRTYASQLQFGWGEKRQILVTPEEYAEAARAAI
                     AEGYNAIKVDPLEIDLHGGDCVFQNKNRNYSGLLLAEQLKMGEARIAAMREAMGDDAD
                     IIVEIHSLLGTNSAIQFAKAIEKYRIFLYEEPIHPLNSDNMQKVARSTSIPIATGERS
                     YTRWGYRDLLEKQSIAVAQPDLCLCGGITEGKKICDYANIYDTTVQVHVCGGPVSTVA
                     ALHMETAIPNFIIHEHHTNAMKACIRELCTYDYQPENGYYVAPELPGLGQELNDEIVQ
                     QYLAYVIK"
     gene            complement(18490..19818)
                     /locus_tag="PN977_002373"
     CDS             complement(18490..19818)
                     /locus_tag="PN977_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000098090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDI6042399.1"
                     /translation="MSSPQKDLYEAISIVKRKIIPLAFILYFFNYMDRVNIGFAALRM
                     NESLGITPEDFANISSIFFVSYLIFQIPSSIGLQKLGARKWISAIIVGWGTVTTLIFF
                     AKDTQHILLARVFLGIFEAGFFPGMVYYLACWFPARERGKVNSFFMLSIAVASVLAAP
                     MSGWIIEHMNTGNYEGWRWLFAIEGIPTVLLGALTFYLLPDRPEKARWLTPSQVTALV
                     DKLNSDNEKAAALNKNTNASFLSIIKNPVLLQLSFAYMFIQAAALAANYWLPGLVKGF
                     SSDFTDTDVGLIMSIPFIFAMFSMPLWGWHSDKKNERKWHAALPMLVAGCGFLMIALV
                     PSMTLRMMGLTLYGVGILSYYGPYWALPSALLSPSGLAISIAFINSCSSLGGFLINKS
                     LGYVSTHYGATGIFIVEAILCFIAVAILLSMKIDNKKDNAQKDNVISHAK"
     gene            20056..20754
                     /locus_tag="PN977_002374"
     CDS             20056..20754
                     /locus_tag="PN977_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001205013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDI6042400.1"
                     /translation="MRDKAKRDLSHISIEIPKSVTQVIKEKIRDMIIHGDLSLGQPIS
                     ENELSNILAVSKTPIREAFILLSHNENLINIIPRSGTFVFSVTDKDINDLIKMRVILE
                     QGAISEAMEKNANNVIIELNSILSKSAKINAERDTQAYLKLDHDFHYVFVKYADNKYI
                     SQAHQLISARLLAIRYRLDFTTEYITNSNRGHATILDMLKNNNVEGVCNFITHHVSSG
                     FTERARKILSLSTQ"
     gene            20832..21554
                     /locus_tag="PN977_002375"
     CDS             20832..21554
                     /locus_tag="PN977_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001091057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-demethylubiquinone
                     3-O-methyltransferase/2-octaprenyl-6-hydroxy phenol
                     methylase"
                     /protein_id="HDI6042401.1"
                     /translation="MNTENLLKNNNVDHEEIAKFEAVASRWWDLEGEFKPLHRINPLR
                     LGYIAERAGGLFGKKVLDVGCGGGILAESMAREGATVTGLDMGFEPLQVAKLHALESG
                     IQVDYVQETVEEHAAKHAGQYDVVTCMEMLEHVPDPQSVVRACAQLVKPGGDVFFSTL
                     NRNGKSWLMAVVGAEYILRMVPKGTHDVKKFIKPAELLGWVDQTSLKERHMTGLHYNP
                     ITNSFKLGPGVDVNYMLHTQNK"
     gene            complement(21693..25457)
                     /gene="yfaL"
                     /locus_tag="PN977_002376"
     CDS             complement(21693..25457)
                     /gene="yfaL"
                     /locus_tag="PN977_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA-I family autotransporter adhesin YfaL/EhaC"
                     /protein_id="HDI6042402.1"
                     /translation="MRIIFLRKEYLSLLPSMIASLFSVNSVAEVLDSCQGYDIKASCQ
                     ASRQSLSGITQDWSIADGQWVIFSGMANNASGGAVFLQQSAEFTISPQNETGMTLFAN
                     NSISGEYNNGGAIFAKENSTINLANVIFDSNVAGGYGGAIYSAGTNDAGDMDLNITNA
                     IFANNIANDGKGGAIYSINNDIYLSDVIFDNNQAYTSTSYSDGDGGAIDVTDNSTDNT
                     HLSGKTIINNTSFTNNYAEGYGGAIYTSSTTSPYLIDISVDDNYDQNNGVMIDENNSA
                     SGYDHSATAAAGGFMYIGHSVAEFNIAADKTLVIGNTSNDGAIDSLAGTGVIVKEGAG
                     ELVLNADNNAFTGEMSIQNGEVTLGRSDELMNVGDTHCQSDPQDCFGLMVGSTVHSEY
                     QAELNVGNTQQTFVHSLTGFANGILNIDAGGNVTVNQGGFSGSIQGEGQLTVAQDGSY
                     LLTGEQSMALTGDIVVEDNAVLSLAGNQADLRAMQSDPQSIVLNGGVLDLSDFTTWDG
                     DSSYNDGLQISGSGGTVIGSNDVVDISSGDDLHIGGSDASQNGVYVVINAGDQRVTLA
                     NNNGYLGNTQIASGTLEVSDNSQLGDTSYNRSVIFTDPQQHSEMDVTTDVDTRSATTG
                     QGRNIEMRADGEIHVEDGVDTQWGGLMADSTGQQLDSVSTLTKSGGGTLELTASGTAT
                     SAVRVEDGTLKGEAENIIPYVSSLWVGEDGVFETGKNQDIRSIDATSGGDIDITDGTV
                     LRLTQQDTNQALDASLFSGDGTLVNATDGVTLTGELNTNLETDSLTYLSDVTVNGDLT
                     NTSGAVSLQNGVAGDTLTVNGDYTGGGTLFLDSELNGDDSASDQLVLNGNTAGNTTVV
                     INPITGIGEPTSTGIKVVDFAADPTQFQNNAQFSLAGSGYVNMGAYDYTLVEDNNDWY
                     LRSQEVTPPSPPDPDPTPDPDPTPDPDPTPDPAPAPTPAYQPVLNAKVGGYLNNLRAA
                     NQAFVMERRDHAGGDGQTLNLRVIGGDYHYTAAGQLAQHEDTSTVQLSGDLFSGRWGM
                     DGEWMLGAVGGYSDNQGDSRSNMTGTRADNQNHGYAVGLTSSWFQHGNQKQGAWLDSW
                     LQYAWFNNDVSEHEDGADHYHSSGIIASLEAGYQWLPGHGVVIEPQAQVIYQGVQQDD
                     FTAANHARVSQSQGDDIQTRLGLHSEWRTAVGVIPTLDLNYYHDPHATEIEEDGSTIS
                     DDAVKQRGEIKVGITGNISQRVSLRGSVAWQKGSDDFAQTAGFLSMTVKW"
     gene            26153..28438
                     /gene="nrdA"
                     /locus_tag="PN977_002377"
     CDS             26153..28438
                     /gene="nrdA"
                     /locus_tag="PN977_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside-diphosphate reductase subunit
                     alpha"
                     /protein_id="HDI6042403.1"
                     /translation="MNQNLLVTKRDGSTERINLDKIHRVLDWAAEGLHNVSISQVELR
                     SHIQFYDGIKTSDIHETIIKAAADLISRDAPDYQYLAARLAIFHLRKKAYGQFEPPAL
                     YDHVVKMVEMGKYDNHLLEDYTEEEFKQMDTFIDHDRDMTFSYAAVKQLEGKYLVQNR
                     VTGEIYESAQFLYILVAACLFSNYPRETRLQYVKRFYDAVSTFKISLPTPIMSGVRTP
                     TRQFSSCVLIECGDSLDSINATSSAIVKYVSQRAGIGINAGRIRALGSPIRGGEAFHT
                     GCIPFYKHFQTAVKSCSQGGVRGGAATLFYPMWHLEVESLLVLKNNRGVEGNRVRHMD
                     YGVQINKLMYTRLLKGEDITLFSPSDVPGLYDAFFADQEEFERLYTKYEKDDSIRKQR
                     VKAVELFSLMMQERASTGRIYIQNVDHCNTHSPFDPAIAPVRQSNLCLEIALPTKPLN
                     DVNDENGEIALCTLSAFNLGAINSLDELEELAILAVRALDALLDYQDYPIPAAKRGAM
                     GRRTLGIGVINFAYYLAKHGKRYSDGSANNLTHKTFEAIQYYLLKASNELAKEQGACP
                     WFNETTYAKGILPIDTYKKDLDAIANEPLHYDWEALRESIKTHGLRNSTLSALMPSET
                     SSQISNATNGIEPPRGYVSIKASKDGILRQVVPDYEHLHDAYELLWEMPGNDGYLQLV
                     GIMQKFIDQSISANTNYDPSRFPSGKVPMQQLLKDLLTAYKFGVKTLYYQNTRDGAED
                     AQDDLVPSIQDDGCESGACKI"
     gene            28584..29714
                     /gene="nrdB"
                     /locus_tag="PN977_002378"
     CDS             28584..29714
                     /gene="nrdB"
                     /locus_tag="PN977_002378"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide-diphosphate reductase subunit
                     beta"
                     /protein_id="HDI6042404.1"
                     /translation="MAYTTFSQTKNDQLKEPMFFGQPVNVARYDQQKYDIFEKLIEKQ
                     LSFFWRPEEVDVSRDRIDYQALPEHEKHIFISNLKYQTLLDSIQGRSPNVALLPLISI
                     PELETWVETWAFSETIHSRSYTHIIRNIVNDPSVVFDDIVTNEQIQKRAEGISSYYDE
                     LIEMTSYWHLLGEGTHTVNGKTVTVSLRELKKKLYLCLMSVNALEAIRFYVSFACSFA
                     FAERELMEGNAKIIRLIARDEALHLTGTQHMLNLLRSGADDPEMAEIAEECKQECYDL
                     FVQAAQQEKDWADYLFRDGSMIGLNKDILCQYVEYITNIRMQAVGLDLPFQTRSNPIP
                     WINTWLVSDNVQVAPQEVEVSSYLVGQIDAEVDTDDLSNFQL"
     gene            29714..29968
                     /gene="yfaE"
                     /locus_tag="PN977_002379"
     CDS             29714..29968
                     /gene="yfaE"
                     /locus_tag="PN977_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like diferric-tyrosyl radical
                     cofactor maintenance protein YfaE"
                     /protein_id="HDI6042405.1"
                     /translation="MARVTLRITGTQLLCQDEHLSLLAALESHNVAVEYQCREGYCGS
                     CRTRLVAGQVDWIAEPLAFIQPGEILPCCCRAKGDIEIEL"
     gene            complement(30022..30672)
                     /gene="inaA"
                     /locus_tag="PN977_002380"
     CDS             complement(30022..30672)
                     /gene="inaA"
                     /locus_tag="PN977_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide kinase InaA"
                     /protein_id="HDI6042406.1"
                     /translation="MAVSAKHDEFNHWWATEGDWVEEPNYRRNGMSGVQCVERNGKKL
                     YVKRMTHHLFHSVRYPFGRPTIVREVAVIKELERAGVIVPKIVFGEAVKIEGEWRALL
                     VTEDMAGFISIADWYARHAVSPYSDEVRQAMLKAVALAFKKMHSVNRQHGCCYVRHIY
                     VKTEGKAEAGFLDLEKSRRRLRRDKAINHDFRQLEKYLEPIPKADWEQVKAYYYAM"
     gene            complement(30753..>31204)
                     /locus_tag="PN977_002381"
     CDS             complement(30753..>31204)
                     /locus_tag="PN977_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDI6042407.1"
                     /translation="LCYAIFLMICRTFTGNVCDKKGPKYVVYPCLVFFTVGLVVLGYT
                     QGSIMMVVSGALIGIGYGSVTPVFQTQIISSVEPHKIGVANSLFFNAMDAGLALGACV
                     MGMMVAHTGYRMIYLLGALLVVVAGGVYELQMKGKSGVMLVAAKEIH"
BASE COUNT         7688 a         8311 c         7392 g         7813 t
ORIGIN      
        1 ctgcaggaac gggaatttga acggattggc ggtcatcaga ccataaaagt tgatatccgc
       61 atcattgctg ccaccaaccg cgacttgcag gcaatggtga aagaaggcac cttccgtgaa
      121 gatctctttt atcgccttaa cgttattcat ttaatactac cgcctctgcg cgatcgccgg
      181 gaagatattt ccctgttagc taatcacttt ttgcaaaaat tcagtagtga gaatcagcgc
      241 gatattatcg acatcgatcc gatggcaatg tcgctgctta ccgcctggtc atggccgggt
      301 aatattcgag agctttccaa cgtcattgaa cgcgccgtcg tgatgaactc aggcccgatc
      361 attttctctg aggatcttcc gccgcagatt cgtcagccag tctgtaatgc tggtgaggca
      421 aaaacagccc ctgtcggtga gcgtaattta aaagaggaaa ttaaacgcgt cgaaaaacgc
      481 atcattatgg aagtgctgga acaacaagaa ggaaaccgaa cccgcactgc gttaatgctg
      541 ggcatcagtc gccgtgcatt gatgtataaa ctccaggaat acggtatcga tccggcggat
      601 gtataacgcc aaaacttgct atgcagaaat ttgcacagtg cgcaattttc tgcatagccg
      661 ctcattctcc ttataaaccc ccatctaatt tatccttttc tattcaatta gttaaataac
      721 aacccccaat aatctcattc tggcactccc cttgctattc cctgactgta cccacaacgg
      781 tgtatgcaag agggataaaa aatgaaaaca aaattgatga cattacaaga cgccaccggc
      841 ttctttcgtg acggcatgac catcatggtg ggcggattta tggggattgg cactccatcc
      901 cgcctggttg aagtattact ggaatctggt gttcgcgacc tgacattgat agccaatgat
      961 accgcgtttg ttgataccgg catcggtccg ctcatcgtca atggtcgagt ccgcaaagtg
     1021 attgcttcac atatcggtac caacccggaa acaggtcggc gcatgatttc tggtgagatg
     1081 gacgtcgttc tggtgccgca aggtacgcta atcgagcaaa ttcgctgtgg tggagctgga
     1141 cttggtggtt ttctcacccc aacaggtgtc ggtaccgtcg tagaggaagg caaacagaca
     1201 ctgacactcg acggtaaaac ctggctgctc gaacgcccgc tgcgcgccga cctggcgcta
     1261 attcgcgctc atcgttgcga cacacttggc aacctgacct atcaccttag cgcccgcaac
     1321 tttaaccccc tgatagccct tgcggctgat atcacgctgg tagagccaga tgaactggtc
     1381 gaaaccggcg agctgcaacc tgaccatatt gtcacccctg gtgccgttat cgaccacatc
     1441 atcgtttcac aggagagcaa ataatggatg cgaaacaacg tattgcgcgc cgtgtggcgc
     1501 aagagcttcg tgatggtgac atcgttaact tagggatcgg tttacccaca atggtcgcca
     1561 attatttacc ggagggtatt catatcactc tgcaatcgga aaacggcttc ctcggtttag
     1621 gcccggtcac gacagcgcat ccagatctgg tgaacgctgg cggacaaccg tgcggtgttt
     1681 tacccggtgc agccatgttt gatagcgcca tgtcatttgc gctaatccgt ggcggtcata
     1741 ttgatgcctg cgtgctcggc ggtttgcaag tagacgaaga agcaaacctc gcgaactggg
     1801 tagtgcctgg gaaaatggtg cccggtatgg gtggcgcgat ggatctggtg accgggtcgc
     1861 gcaaagtgat catcgccatg gaacattgcg ccaaagatgg ttcagcaaaa attttgcgcc
     1921 gctgcaccat gcctctcact gcgcaacatg cggtgcatat gctggttact gaactggctg
     1981 tctttcgttt tattgacggc aaaatgtggc tcaccgaaat tgccgacggg tgtgatttag
     2041 ccaccgtgcg tgccaaaaca gaagctcggt ttgaagtagc tgccgatctg aatacgcaac
     2101 ggggtgattt atgattggtc gcatatcgcg ttttatgacg cgttttgtca gccggtggct
     2161 tcccgatcca ctgatctttg ccatgttgct gacattgcta acattcgtga tcgcgctttg
     2221 gttaacacca caaacgccga tcagcatggt gaaaatgtgg ggtgacggtt tctggaactt
     2281 gctggcgttt ggtatgcaga tggcgcttat catcgttacc ggtcatgccc ttgccagctc
     2341 tgctccggta aaaagtttgc tgcgtactgc cgcctccgcc gcaaagacgc ccgtacaggg
     2401 cgtcatgctg gttactttct tcggttcagt cgcttgtgtc atcaactggg gatttggttt
     2461 ggttgtcggc gcaatgtttg cccgtgaagt cgcccgccga gtacccggtt ctgattatcc
     2521 gttgctcatt gcctgcgcct acattggttt tctcacctgg ggtggcggtt tctctggctc
     2581 aatgcctctg ttggctgcaa caccgggcaa cccggttgag catatcgccg ggctgatccc
     2641 ggtgggcgat actctgttca gtggttttaa cattttcatc actgtggcgt tgattgtggt
     2701 gatgccattt atcacccgca tgatgatgcc aaaaccgtct gacgtggtga gtatcgatcc
     2761 gaaactactc atggaagagg ctgatttcca aaagcagcta ccgaaagatg ccccaccatc
     2821 cgagcgactg gaagaaagcc gcattctgac gttgatcatc ggcgcactcg gtatcgctta
     2881 ccttgcgatg tacttcagcg aacatggctt caacatcacc atcaataccg tcaacctgat
     2941 gtttatgatt gcgggtctgc tgctacataa aacgccaatg gcttatatgc gtgctatcag
     3001 cgcggcagca cggagtactg ccggtattct ggtgcaattc cccttctacg ctgggatcca
     3061 actgatgatg gagcattccg gtctgggcgg actcattacc gaattcttca tcaatgttgc
     3121 gaacaaagac accttcccgg taatgacctt ttttagttct gcactgatta acttcgccgt
     3181 tccgtctggc ggcggtcact gggttattca gggacctttc gtgatacccg cagcccaggc
     3241 gctgggcgct gatctcggta aatcggtaat ggcgatcgcc tacggcgagc aatggatgaa
     3301 catggcacaa ccgttctggg cgctgccagc actggcaatc gccggactcg gtgtccgcga
     3361 catcatgggc tattgcatca ctgccctgct cttctccggc gtcattttcg tcattggttt
     3421 aacgctgttc tgacggcaac cctacagaca gaaggaatat aaaatgaaaa attgtgtcat
     3481 cgtcagtgcg gtacgtactg ctatcggtag ttttaacggt tcactcgctt ccaccagcgc
     3541 catcgacctg ggggcgacag taattaaagc cgccattgaa cgtgcaaaaa tcgattcact
     3601 acacattgat gaagtgatta tgggtaacgt gttgcaagcc gggctggggc aaaatccggc
     3661 gcgtcaggca ctgttaaaaa gcgggctggc agaaacggtg tgcggattca cggtcaataa
     3721 agtgtgtggt tcgggtctta aaagtgtggc gcttgccgcc caggcaattc aggcaggtca
     3781 ggcgcaaagc attgtggcgg ggggtatgga aaatatgagt ttagcgccct acttactcga
     3841 tgcaaaagca cgctctggtt atcgtcttgg agacggacag gtttatgacg taatcctgcg
     3901 cgatggtctg atgtgcgcca ctcatggtta tcatatgggg attaccgccg agaacgtggc
     3961 caaagagtac ggaattaccc gagaaatgca ggatgaactg gcgctacatt cacagcgtaa
     4021 agcggcagcc gcaattgagt ccggtgcttt tacagccgaa atcgtcccgg taaatattgt
     4081 cacccggaag aaaaccttcg tcttcagtca agacgaattc ccgaaagcga attcaacggc
     4141 tgaagcgtta ggtgcattgc gcccggcctt cgataaagca ggaacagtca ccgccgggaa
     4201 cgcgtctggt attaatgacg gtgctgccgc tctggtgatt atggaagaat ctgcggcgct
     4261 ggcagcaggc cttacccccc tggctcgcat taaaagttat gccagcggtg gcgtgccccc
     4321 cgcattgatg ggtatgggac cagtacctgc cacgcaaaaa gcgttacaac tggcggggct
     4381 gcaactggcg gatattgatc tcattgaggc taatgaagca tttgccgcac agttccttgc
     4441 cgttgggaaa accctgggct ttgattctga gaaagtgaat gtcaacggcg gggccatcgc
     4501 gctcgggcat cctatcggtg ccagtggtgc tcgtattctg gtcacactat tacatgccat
     4561 gcaggcacgc gataaaacgc tggggctggc aacactgtgc attggtggcg gccagggaat
     4621 tgcgatggtg attgaacggt tgaattaacc aataaaaaca cccgatagcg aaagttatcg
     4681 ggcgttttct tgaacatcga cggcgaaggt aaccccatta atcaccagtc aaaacttttc
     4741 accagcgtca gctcgccagc attacgcatc ggtacaataa atgtttcctg tttctcattg
     4801 accgatcctt catcggtgat cagcgtcagt tgggcggtgg ttaattccgt ttcgctgcgt
     4861 ccgccatagt agttgatata cacctgatag cgcccgtgaa ctggcgctgg catggcgaaa
     4921 atctcggggc cgtaccccgt cgtgacatcc atatccagtg caccactgtt gttcagcacg
     4981 gtgttaccgt accaggcgtg ttcgccatcg ggcgtaacaa cgtgaaggtc gaggtcggta
     5041 tcgtccgtat cccacgagag aaccagtcgt aaacgcgcgc ggatcactcc agcacccggc
     5101 gttgagtaaa actgcatttt ttgccggctt tgcccatccg ggctgatgac ctgcacgctg
     5161 ttgctgcctt ccgtgaaaat ataagggcgt gcaaatgaac catcggattc aattctctgc
     5221 ggcatactgg caccgttgac caccagccgg ccctgctgaa ctttacccgc cgcagcataa
     5281 ttctttattt tgccgcggat ctgcgctgag atattttgat catctgccat gttgacggac
     5341 gacgccgggt aattgatcga ttgtgaaaaa ctggcatctt cgccctctgc cggatgccag
     5401 ccagagagcg gtgcatcaat ctcgacttcc tgcacacctt cactgtgagc gacaggcgaa
     5461 agcgccacca gtaacaacgg aagaaaaatc tttcgcatag cattagtcca ttaataattg
     5521 tcgggtcagc gtttcgatat agttctcatc aagcccggtg ggataaccat caaaggccag
     5581 gtgcagatac tcatgggtta aatccagccg atcctgaagc gtaaagaagt tacgaataaa
     5641 cagccgtttc tgctgccgat cggtataggg gaaacctgag actaaacgac agaccgcaaa
     5701 cacgtctggt tcgttgtagc ccgtctcgcc ttgcaatata cgccgccact gcggcatttt
     5761 tttcgccagc caggctttcg ctttgggtaa taactgacag gttgagcgtg gcgctcccca
     5821 gcggctaagg ctgttatcgg gataagcaaa cgcgagtatc tggtcgtaac gctctccctg
     5881 ccccgcctgg gccgtggctt gccgccagga aagcgtccct tcggtagcgc gactgccgtg
     5941 ataatgaacc ggatcgcccg catagataag atcctgcgtc caggcggtca tcgttcgcgc
     6001 ccccgtggtc gctggcgaag ccgaaacacg ctgtgtggcg ctgctgtcag agatggtcag
     6061 gcaatcgcct tcccgattgg cgttttgctg taaaaaagtg cgtatcgcca cggtcatggc
     6121 tttcgctgcc tcagggggcg tacttttcgc ctcgcgatcc agaactctgg caacatactc
     6181 ttcacgatcg agatgcgact gcaatttcag tttgcccttt tcgctcagta aggtcgtctc
     6241 accgtggctg acaaacgtaa tgtggttacc attggcgaac gtcacacgat agcgcccatt
     6301 aagtacgccg ggttttactg ccgtcgtact cttttccgct gcgattttct tcagcggata
     6361 gcgggcaaac agctccacca ggacacattg cccactggca acctgcgtcg gcactggcaa
     6421 aacccgatta agcaccgtcg cataacgggt cagtaccgtt ttgctggtgc cactgccagt
     6481 cacccacagc ggtgtaccat cggttaacca gccggcaaaa ccaccctgac ggatttcctg
     6541 tttatcatcg gcaaaccagc tccaggtttt cacccgtacc cgactaccga gcatggatgc
     6601 gacaccgatt ttcgcctcgt ccagcaccac atccagtaaa acttcctgag ctttgttttg
     6661 cgcaggtaac gtcgctaatg aatcaagcag cgatttaacc gttaccgagg tttctggctt
     6721 cagcgtagta agagacgcta accacgcagg tgcctgacgc tgttgccaat attgccccca
     6781 cacatctgcg ccaatatgca accgctgcgg cgcaaaatag agcccacagg aacgtaccag
     6841 cgcggtatcg cgcgttatac tctccccagc ctgacaacaa taaacctctt ccggggagtt
     6901 tccgcgacac tggtagggct gctccggttg atgggtatcc tccagccagg cgtaaacata
     6961 cagtttccac aaactcccta acggcgtggt gagcgtgtca ggtaatgccg agactttggt
     7021 cacgcctgac gacgataact gatacagttg gtcatgctgc gcgccccgca gcgccagttg
     7081 taacgacgtc tcctccgcaa gcgttggcag agtaaccaac gctaatagcc agacgattct
     7141 gcgccagttc acttatttca cctgcatccc ggtccattcg ctccctgccg caacactttg
     7201 ctgtgcaggt gcataggaac gcacataacg agcaggaggc agaacgaatt gccctttttg
     7261 cgagaagcgc agcaaatggc ggaaagtgac cgttcccgtc agttctttca ccggcaccat
     7321 ataggccagt tcgcccattt catttcgcgc tttttccagc aactgcccct gctgttttcc
     7381 agcgttcggt ttattgaccg aaatgcccca cgtagtcctc tcaacgtcag cccctggcgg
     7441 gagcggtact tccacctgac cgtagcgcag aacggcatcc tgctcgctgg taagcgtaat
     7501 ttcatcgaga tacagcgcat cgctgtcaat ctcattgctg gtcaccggct gcagagtaaa
     7561 gctcatctct tcttcaccgg ggataagccg atacaactgg cgttcaacgg tcaccggaat
     7621 gttactttgt tgagccgttt ttgccggttc acgccagcgg acctgcacat tttgcggcga
     7681 caattcatca ccaaaagaga gaatgtccgg cacgccctga ccaacccaac gccagtattc
     7741 gccccctcca gttaacttat gtttggccca tgcgcccgca ggcgcaggca acacaaccgg
     7801 aggcattgtc gccatatatt tcgccagcca gttcatggcc agcgcgcgct caatagtgga
     7861 ttgctcagcg gtcaaaccac ttaaaatagc agcagcctgg gtagcgtcgc ccccgctatt
     7921 gagcagcaca accgtgtgcg ccagcggctg attgctgttt gccgctttat cccacgcata
     7981 ttgctgaacc tgtggcatca cagtggactt caactgcgct tttttcagca acgtcatggt
     8041 taagacgttt gccaccgcat ctgccagtgg agattcaggt gtatcgagga tcagactgtc
     8101 attgatatcg ctggtgtctg cctcagagaa atctttagtt ttagttccgc gccgggcgat
     8161 agcttcatcc aaccctttca acaacgtttt gcacggcaga ttcatctcct gcgcccacgc
     8221 cagcaccagc gcccgatgca ataacggcat gttatctgcc tgctcggcat aactgtcgag
     8281 catatgctgc cagtattccg gttgttgcgt tacgccgagc gcctggctgg cctgccagtc
     8341 ggcgtaccat gcccatgccg tgaggaaggc gtcaccattg ccatcttcac cccaccaggt
     8401 aaagcgcgca ccgggccctg ccagttgcat cagccgcaga cggttatcct gaatcatctg
     8461 acgaatgtcg ttagcggcgg cactttgatg atcggcaagc gaacgccagg cgagactgat
     8521 cgggatcaga cggctaccgg tgttgatcac gccaccccac ggttcatcga ctaacgcatc
     8581 aagattgtta cggaaaatct cctgcggcgt ttcactactt tgtagccgga tattgctcgc
     8641 ctgctcgggc aacatcagcg cattatcgcc accgccgagc atgacatttt tctgctgttc
     8701 aacgggccag ctgttatcca caaaagacag ttttgtgcta atgctgtcct gcacttgccc
     8761 attttgttgc agttctgcgc ttaacaagcc agattgctga atattctgcg tcagcgaaat
     8821 ataattcgcc cctttgtgca gcgtcagcgt ctggcgcatc tcagcgcctg caaatttagt
     8881 caccagcgcc accggttcgt tatcctgctg actgaagata aacagtcctg ccgccggttt
     8941 gtcgcccacg cgatacaccg ttggcatact ccacttcatg tagagatttt tttccgaacg
     9001 cagataagca cgcccctgcc cgaccagccc gtcgccgttc atcccacgcg cggtgatacg
     9061 ccagcgggtt aacgaatcag gcatcaggaa cgtgaaatac gctttgcctt gtttatcggt
     9121 tgtgagtgac ggcatccatg ccgcggtatc cacctcttca cgccgtggac gttcaagcat
     9181 ttttactcgc cgctcgctgc ggttagttgc gccaggcgca accggctcgc tggagagcgc
     9241 ctggtcgtag ctgataaacg acaaactgga gctggtacgc acattgttac gccccagcgg
     9301 atagaaaaat ttgccgatat tcggcgcgat ttccggttgc agcgcgtaga tcatttcatc
     9361 gaccacgcct accgttaact gcgcagaaac aggtttacct ttcagcgacg aggttaattc
     9421 gacattgacc agttcaccag gctggtaatg ggttttgtcc gttttcaccc ggatatccag
     9481 ctggggaacg gcaactttga tcccggcgtt ctgaaaactg tactgaccgt tacgggtata
     9541 cagcaccgaa aaagtgatgt taggcgcaaa ggaattgctc actggaaccc gggcttcata
     9601 ctgggtatcg tttaaacgtt gtaacgtcag ccagtttgcc ggatgcgaaa gcagcgactg
     9661 ctgttccacg cgatcgcgtt ccagcgtcaa taatgcttca tcaattggct ccggaaaggt
     9721 aatcagcatc ttcgcagttt cgcctggctg atacagcgtt ttatccgcca cgatatctac
     9781 cgtaccagta tgcgccgtgc tgcccttacc gctgacggca tgacttaacc cagcgagaat
     9841 taagccgtct ttatcgcgta atgtcagatt gtagttgcca ggtttagcga aattgacggt
     9901 aaaggattgg ccgcctgacg gtagctctcc gctatggctc gtgcggtctt cgagacgcaa
     9961 ccattcatac gtaacaggaa cctgttttga agattccagc gcggcataac ggaacacaac
    10021 cgattcaccg ctattactgt attgtgcggc agtacttaat gagtaatgtg ccagtccacg
    10081 ttcaatgagg atctctttgg tggtggtgac acgatacgcc gcaccgtcac tggcggagac
    10141 ggttaacaaa tagcggcttg gtttatcagc ggcggggaga tttaacgcca catgaccgct
    10201 atcgtcggat accgtttcac tgccttccag cgacacggga aaacgtccgg cataacgcaa
    10261 atcgttaccg accattgata attgctgagc gcgcaaactt aactgcacac gggcatcttt
    10321 taccggctca ccatccgggt agagcagttg cagtttgccg ctgaccgctt cgccagtttt
    10381 gaactctttt ttgtcgagag ctaagccaat ctcgaaatgt ggcttgatgt agtttgccac
    10441 gcgaaaacta ctgctataga cctgattgcg gtaagcaaga cgcaactcat aacctccggc
    10501 aacggcattt tctggcaggc ggaaactccc ctgcccgcca ttgcgcgcat ccagcgtgac
    10561 attgatggtt tgcaacagac tgccgttagc gtccagcacc gaaagcttcg ccggggcgct
    10621 gacgatgggg gatgaatgca acggatcgtg gaactcgcgc cccatcactt taacatcgac
    10681 acgatcgcct gcgcgatata gcgggcgatc ggtaaagata tacaagcggg tgttgtagat
    10741 ttcactttcg tagaaaaagt tctcggagac aaaaacgccg ccttcagcat ccttacccag
    10801 aatgtatgaa cgttctggcg atatatgttg taactgcaag gtaccgctgt catcggtcac
    10861 accgcgagtc atcacgccaa gaccgtcagt ccacaggatc tctgagcctg gcttcgcttc
    10921 accctgtttt ttgcccgcgg tccacaccag aagttcgttg cctgacactt tgctaagcgc
    10981 cactgtatcg gagacaaata ccaccgtcgt tgcccgatac ccaccgacca tcgcttcgac
    11041 gagatacagt cccggctctt gtttgccgag aggaatatag atgttacccg gctgcggcga
    11101 gatgaaattg ctggatgcgc cttccagttt taccccttgc tgcggctcga ctggtttagc
    11161 ctgccataat gggtaacgga actgttccac cagtggatat tttttcagcg gtgaaaactg
    11221 gctgttctgt acataacggg aaggtttaac aatggcatta ccgagctgta attcgggtaa
    11281 cgcctgagtc acattctgcc gtgactgaga agagaaagta cgctgcatta cacggcgaga
    11341 tttgccatac cagttatccc acagccaggt cagcgtattg tttagcccgt cgcccagata
    11401 tttcggttgc accacaatgc gatgcaggtt tttctgctgg cgcaaaaatg ccatcgggtc
    11461 aggaatacga tacaggcgaa cgtccacgcc accgtactct tccatctgat agcgtcgata
    11521 atcacgcccc ggcgcttcca gtcgcacttt cgcctcttca ctgctgctaa aactgctgtc
    11581 agcgagcaaa aagaatgttc ccccgccggg cggcgcatag ttactggaag gaagcgaatc
    11641 atcagcatta gcaagccctg ttcccacaag agacaggcaa aagcacgctg ctatcgcgcc
    11701 agaaaattta aacgataaat gccaatgaaa ttgggattgg aatcgttggg tatccatcgg
    11761 gtgtccttcc atgtcataag ttgttgcaga ctgactgcgc gcattccgtt gtcagttttc
    11821 gtggcgcttc cggtgtggta gatgacgtaa cgccccatcc agaccattaa gtgctgggca
    11881 tcgccctgat cgaaaaaaat catatcgcca ggcagcgcct ggtttatgtc ctggccaata
    11941 aattggctgt tgtactgaat caaattaatc gcggtcacat aggggccagt ttttccgttc
    12001 ccatgattcc agttttgcgc cagttgacgc tgttcaggtg ttagcgtcat ttcaggcggc
    12061 aaatattgac ttgctatacc gttacttttt agccatttgc tgtcgtgaat tttcagcgcc
    12121 tcgttcgccg aaaatcgcac caggcccgca caatcctgct gataccagcg cggacttggc
    12181 ccctggcgga gctgttcctg tgcaatgcgt acaaaccacg cgcgaaacag cccggattgt
    12241 tcgacattca gcatttcgct atgggcaaca acacaacaca gccagcaaat cagcgccagc
    12301 agtccgtgcc tcatagcggc tgccaggtta tgggtagcca ctgccaggcg gcaccgggtt
    12361 ccatctgggc cagcttcatg acataacgcg gttgttgaga taaagcgtcc agcttcggca
    12421 ttaataaagt ttgtgcggcg ttataaaaaa ccggttcgag attcttcggc aaactggtca
    12481 gcgtttcgtt acgcagcagt ttcgccacgc cttgtggatt gatatagagc ggaacgatgc
    12541 cttcagtggg tatcacatcc accattgccg ggcgggtttt attcagtgtt tgcagcgcgt
    12601 tattaaccag cgtgtcatcg agggagaaaa gcagcgtttt gttttgcatc gccagcgaca
    12661 cgcggaaaaa ataatccgac attaattgat cgggttgcgc cgcctgcgct ttcggatact
    12721 ggccgtatcg ggaactcact tcgcgacgcc agatttgcgc ttcgccctgc tgagtctggt
    12781 ttaccggcaa aacaccttct ggcgctttgc tttcgtgcgc accaatattt tgcgtaaaca
    12841 gttttcctgg caattgcact tgttcggcag aaccatcaaa ctgaccgaca aacagcggag
    12901 tttgcaattt tgaatcttca taccagcaca gcccggctgc accatctaac gccccgttca
    12961 gcttgtcgtt ttcctggctg atgtgcgaaa gcatctcttc ggcaatacca tgcgaatacg
    13021 gcaccgccac acagaagctg gccccggcag gcatactgtt ccataccggc gtaaaatcga
    13081 aactggcatc tacgctggcg gattcatcat ttaacgccac aaagctatgc cagctgtcgt
    13141 tgctcatttc gaagcgtacg ccagcaaaag aaggcattaa tcgctggtag ccaaacccca
    13201 gcaacctggc gctgaccacg atgcgctggc gtactggcgt tttttcagca gcacgctctt
    13261 ccaggccaaa gctggcttgc cagcgttttt taccgctcaa caaatcactt gcgatcgccg
    13321 tggcttcggt atcctgctga tcatctttaa acagcatatc cgtgctggaa aataccagca
    13381 tcttgtcctg ataagttgcg aacatcaggg cgttattgcc gttatagcgc aactgataaa
    13441 ccgggatggt ttcactattt agcttaatac tactgatttc cgttttactt aactggctgt
    13501 cgctggtagc ggcaaacagc aatggctcca gcagcttgct taaaccactt cgctggatca
    13561 gcaccatata atgagaaaga tggccttgtt tatcgtgcca tagcgccgcc tgcgcgggct
    13621 gatctaagag tgacgaaaag agcttatctt ttagtgtgag atcgtgttcg tagacaatgc
    13681 ggcgaatgct gccttcaatg cccagacgat cggcatgatt ctgataatag aaaacgaaat
    13741 cttcactcag aacatcgtgg agaaacggaa tggtaaggag gtctttggga agctggctca
    13801 gagagtcgct gtcgagaaag aggtccggct cattgagatc gatttgcaga ttgttgtgca
    13861 ccaccagcgg cgacaacgtt ttttctggcc cactgccagc atattgcaac gcccagacgc
    13921 cagcggaaag cagtactatt gcgcccaaac ctacaagacc atagaaccgc cagcctttcg
    13981 cctttttttc accactcatt gccacattcc ttgtgtaaag ccagccattt tttacgagca
    14041 cagcaaaact ttaccgcgcc ctaatacgac aaaagcccag actttgcagc ctggactttt
    14101 caattcaaac aagggagata tctccctttt ggcatgaaga cgtaaaatta ttcttcttct
    14161 ggctcgtcgt caacgtccac ttccggagcg atttcatcgt ccccttccgc ggcactgccg
    14221 tcgatggtat ccagatcttc ctcgtcaacc ggttcagcaa cacgttgcag acccactacg
    14281 ttttcatctt ccgcagtacg gatgaggatc acgccctggg tgttacggcc cacgatgctg
    14341 atttctgaaa cgcgagtacg taccagcgta ccggcatcgg tgatcatcat gatctggtcg
    14401 cagtcatcta cctgcaccgc gccaaccact aaaccgttac gttcggtaac cttgatggag
    14461 ataacccctt tcgtcgcacg cgacttggtt gggtattccg ccactgcggt acgtttacca
    14521 taaccgttct gcgttgcggt gaggattgcg ccatcaccac gaggtacgat cagagagacg
    14581 actttatcgc cttcgcctaa acgaataccg cgaacaccgg tggtgttgca gcccatcgca
    14641 cggacagaag actctttaaa gcgcaccact ttaccttcgg cggagaacag cattacttca
    14701 tcttcgccgc tggtcaggtc aacgccgatc agctcatcgc catcaaccag tttgatcgcc
    14761 actttaccgg cggtacgcag acggttgaat tcggtgagga cggttttctt cacggtcccg
    14821 ttagcggtgg ccatgaagac tttcacgcct tcttcaaact cggtcaccgg caggatcgcg
    14881 gtgatacgtt cgtcctgctc cagcggcagc aggttgacga tcggacgacc gcgcgcgcca
    14941 cgagtagctt ctggcaactg gtaaaccttc atcgaataga cgcgaccacg gctggagaag
    15001 cacagaatat ggtcgtgggt attcgccacc agcagtcggt cgataaagtc ttcttcttta
    15061 atacgtgcgg cagatttacc tttcccgcca cgacgctgcg cttcgtattc agaaagcggc
    15121 tgatatttaa cgtagccctg gtgagagagc gtcacgacca catcttcctg ggtgatcaga
    15181 tcttccaggt tgatgtctgc gctgttggcg gtgatttcag tacgacgttt gtcaccgaac
    15241 tgttcacgaa ccagctccag ctcttcgcgg atcacttcca tcagacgatc ggcgctacca
    15301 agaatacgca acagttccgc gatctgatcc agcagctctt tgtattcgtc gagcagtttt
    15361 tcgtgctcaa ggccggtcag tttctgcaaa cgcagatcca gaatcgcctg agcttgctgt
    15421 tcggtcaggt agtacagacc gtcacgcacg ccgaactctg gctccagcca ttccggacgc
    15481 gcagcatcgt cgccggctcg ttccagcatc gcggcaacgt tgcccagctg ccacggatta
    15541 gcaaccagcg ccgttttcgc ttctgcaggc gtcggcgcat gacggatcag ttcgatgatc
    15601 gggtcaatgt ttgccagcgc gacggctaat gcttcaagga tatgagcacg atcgcgcgct
    15661 ttacgcagtt cgaaaatagt acgacgggtc accacttcac ggcggtgacg aacaaacgcc
    15721 gcgatgatgt ctttcaggtt catgatcttc ggctgaccat ggtgcaatgc caccatgtta
    15781 ataccgaaag aaacctgcaa ctgggtctgg gaatagaggt tgttgagcac cacttcaccg
    15841 accgcatcgc gtttcacttc aatcacgatg cgcataccgt ctttgtcaga ctcgtcacgc
    15901 agcgcgctga taccttccac gcgtttttct tttaccagtt ccgcaatttt ttcgatcagg
    15961 cgagctttgt tcacctgata cggaatttcg tggacgataa tggtttcacg cccggttttg
    16021 gcgtcaactt ccacttccgc acgggcgcgg atatacacct tgccacgacc ggtacggtaa
    16081 gcttcttcaa taccgcgacg accgttaatg attgccgccg tcgggaagtc cggccccggg
    16141 atgtgttcca tcagcccttc aatgctgatg tcttcatcat cgatatacgc cagacaaccg
    16201 ttgatgactt ccgtcaggtt gtgcggcggg atgttggttg ccatacctac ggcgataccg
    16261 gaagaaccgt tcaccagcag gttaggaatt ttggttggca tgacgtccgg aattttttcc
    16321 gtgccgtcat agttatcaac gaaatcgacc gtctcttttt cgagatcggc catcagttca
    16381 tgggcaattt tcgccagacg gatttccgta taacgcattg ccgccgcaga gtcgccgtcg
    16441 atggaaccga agttaccctg accgtctacc agcatgtaac gcagcgagaa tggctgcgcc
    16501 atacggacga tcgtgttata aaccgccaag tcaccatggg gatggtattt accgattacg
    16561 tcaccaacga cacgggcaga ttttttatag gctttgttcc agtcattgcc tagtacgttc
    16621 atggcgtaaa gtacgcgacg gtgtaccggc ttcaggccat ctcggacatc cggcagcgca
    16681 cggccaacaa tgaccgacat cgcataatcc agataggagc tcttcagctc ttcctcaatg
    16741 ttgaccggtg taatttctct cgcaaggtcg ctcatctaac cgctatccct ctactgtatc
    16801 ccggattcaa aggtcggaga ttataacaca atcgcttgcg gacgggtaaa cgtaatgcag
    16861 gcttccgggc gctgtagatc tactgcgttt taccccaccg ccagcacaca tcgcgctggc
    16921 ggtgaacgac aaaggaacaa agaccgcaac acgcttcctg tgcctcaata aacaccagaa
    16981 ataatggcta tactcgtcat acttcaagtt gcatgtgctg cgtctgcggg ttattcggcc
    17041 catccatgga cctcacccct tacggggccg ctgcaagcag cgttcaaatc cgctccatgc
    17101 ggatttgtcg ctcaccccag tcacttactt atgtaagctc ctggggatta atgagagaca
    17161 tccatgtctc tcaccgaagg ccagcctgcg gctgggcaaa ttcgttcctg acgaatttgt
    17221 cactcgcttg tcgccttcct gcaactcgaa ttatttagag tatataaatc gccgttattt
    17281 gatcacatag gcaagatatt gttgtacgat ttcatcgttt aattcctgac caagcccagg
    17341 caactctggc gcgacataat agccattctc gggttgataa tcgtaagtac aaagttcccg
    17401 aatacatgct ttcatcgcat tggtatgatg ttcatgaata ataaagttag gtattgcagt
    17461 ttccatatgc agcgccgcta ctgttgaaac aggcccgcca caaacatgta cctgaacggt
    17521 tgtgtcatat atattggcat aatcacaaat tttctttccc tctgtaatgc caccacaaag
    17581 acacaaatca ggttgagcta ccgcaattga ttgtttttcc agtaaatcac gatatcccca
    17641 gcgagtataa gaacgctcac ctgttgctat cggaatcgat gttgaacggg caactttctg
    17701 catattatcc gaatttaatg gatgaattgg ctcctcatag agaaaaatac gatatttctc
    17761 tattgctttg gcaaactgga tcgctgaatt tgttcctaga agagaatgaa tctctacaat
    17821 aatatcagca tcgtcgccca ttgcttcacg catagcggca attcttgcct cgcccatttt
    17881 taattgttcg gccagtaata agccagagta atttctattt ttgttctgaa agacacaatc
    17941 gccgccatgc agatcgattt caagcggatc aacttttatc gcattataac cttctgcaat
    18001 agcggcacgc gctgcctctg catactcttc aggcgttact aatatttgcc ttttttcgcc
    18061 ccaaccaaat tgtaactgac tggcgtaagt tctcaattta tcgttagtct ttccccccag
    18121 tagttgataa accggcacgt ttaaatattt acctttaata tcccataagg caatatcaat
    18181 agcgctcata cctgcgtaaa aaacattacc accgcccatt ccccaaaatg ttttcctgaa
    18241 gaggaattcc caaatcttct caatgttcaa cggatcttca ccaatgatga gaggggctaa
    18301 atctctgata ataccaacac cagattttgc acctgcacca taggctaatc ctacttcgcc
    18361 aacaccactt attccagcat cggtattaac ccgaataagt actggattat aagaggccat
    18421 agtttgatct ctttttttga gttcgcagtc aaaaatttct acagatgtaa tcttcataaa
    18481 taacctcatt tatttagcat gtgaaataac attgtctttc tgggcgttat cttttttatt
    18541 atcaattttc attgataaca aaatagctac ggcgataaag caaagaatcg cctcaacaat
    18601 aaatatcccc gtggctccat aatgtgtaga aacatagcca agtgatttat tgatcagaaa
    18661 accacccaaa cttgaacaag agttaataaa cgcaatactg attgccaggc ctgatggtga
    18721 aagtaaagct gagggtaaag cccagtacgg accgtaataa ctgagaatgc ctacaccgta
    18781 taatgttaat cccatcatgc gcagcgtcat cgaaggtaca agtgcgatca ttaaaaaacc
    18841 gcaacctgca accaacatcg gtagtgcggc atgccatttc ctttcattct ttttatcgga
    18901 atgccatccc cataatggca tactgaacat cgcgaaaata aacggaatac tcataatcaa
    18961 accgacatcg gtatctgtga aatccgatga aaatccttta accaaccccg gtaaccagta
    19021 atttgccgcc aacgccgctg cctgaataaa catataagca aatgagagtt gtagtaatac
    19081 tggattttta atgattgaca gaaaagatgc atttgtattt ttatttaatg ctgcggcttt
    19141 ttcgttatca gaattaagtt tatcgaccaa tgcggtgact tgcgatggtg taagccagcg
    19201 ggctttttcc ggacgatctg gcagtaaata aaaggtcagc gcccccaata acaccgtagg
    19261 aatgccttca atagcaaaaa gccagcgcca tccttcatag ttaccggtat tcatatgttc
    19321 aataatccaa ccagacattg gtgccgccag cacagatgca actgcgattg ataacataaa
    19381 aaagctattc acttttccac gttcacgcgc cgggaaccaa catgccaggt agtaaaccat
    19441 gccagggaaa aaccccgctt caaatattcc caaaaagacc cgagcgagca atatatgctg
    19501 ggtgtctttg gcaaagaaaa ttaacgttgt tactgttccc caaccaacaa taatggcgct
    19561 aatccatttt cttgcaccta acttttgcaa tccaatactg ctcggaattt gaaaaatcaa
    19621 ataggacaca aaaaatattg aagatatatt agcaaaatcc tctggtgtaa taccaaggga
    19681 ttcattcatg cgtaaagcgg caaatccgat gttaacacga tccatatagt taaaaaaata
    19741 cagaataaat gctaacggaa taatcttcct tttcacaata gaaatggctt catacaaatc
    19801 tttctgagga ctgctcattt tgtttcacct tttgtggttc aatgaataag agtactgccc
    19861 gaagtggata ttacgtaaaa aataatccag ttcaaaaggt gatgtgttat atctgatata
    19921 tcagcttgat cattgattga aacactaagc tacaacaata agatatatca tttttgtcat
    19981 atctgatata ttagatacct tcattgataa aatgattaca tataatggac cagcagctta
    20041 ataaggggca aaggaatgag agataaagcg aaacgtgact taagtcacat atcaattgag
    20101 ataccaaaat ccgtaacaca ggtcatcaaa gaaaaaatca gggatatgat aattcatggc
    20161 gatctcagtc ttgggcaacc gataagtgaa aatgaacttt cgaatattct ggcggtaagt
    20221 aaaacaccta ttcgtgaagc ttttatttta cttagtcaca atgaaaatct gatcaatatc
    20281 attccacgca gcggaacatt cgttttttcc gtaacagata aagatattaa tgacctcata
    20341 aaaatgcgag taattctgga gcagggtgca attagtgaag caatggaaaa gaatgcaaac
    20401 aatgtcatca ttgaactaaa tagtatacta tcaaaatcag caaaaattaa tgccgagcgc
    20461 gatactcagg cttatcttaa actggatcat gattttcatt atgtgtttgt taaatacgct
    20521 gataataaat acatctctca ggcacaccaa ttgatatccg cacgtttact tgctatccgt
    20581 tatcgactgg atttcactac ggaatatatc accaattcaa atcgtggcca tgcgactatt
    20641 ttagatatgc taaaaaataa taacgttgaa ggtgtttgta attttattac tcatcacgta
    20701 agtagtggat ttaccgagcg cgcacgaaaa attctctcgt tatctacaca gtaaaatgtg
    20761 aatgagttta taacactggc acttttcacc tgctatactg cccgacaacc tttatagact
    20821 cttatgtaac aatgaacact gaaaatctcc ttaaaaacaa taacgtagac cacgaagaga
    20881 tcgctaaatt tgaagccgtc gcctcccgct ggtgggatct ggaaggtgag ttcaaaccgc
    20941 tgcaccgtat taacccgctg cgtctgggct atattgccga gcgtgctggc ggtttatttg
    21001 gcaaaaaggt gctcgatgtc ggttgtggcg gcggcattct ggccgagagt atggcgcgcg
    21061 aaggtgcgac ggtgaccggt ctggatatgg gctttgagcc attgcaggtg gcaaaactgc
    21121 acgcactgga aagcggcatt caggtggatt acgtgcagga aaccgtggaa gagcacgcgg
    21181 caaaacatgc cgggcagtat gatgtggtga cctgcatgga gatgctggag cacgtccccg
    21241 atcctcagtc agtggtcaga gcctgtgcgc aactggtgaa accgggcggc gatgtctttt
    21301 tctcgacgct caaccgcaac ggcaagtcat ggctgatggc ggtggttggt gctgaatata
    21361 ttttgcgcat ggtgcctaaa ggtacacatg atgtgaagaa gtttattaaa ccggcagaat
    21421 tgctgggctg ggtggatcag accagtctta aagaacggca tatgaccggg ctgcattaca
    21481 accctataac taattccttt aagctcggcc ccggcgttga tgtgaactat atgctgcata
    21541 cgcagaataa gtgaggttgt tgtttggatt catccgacga ccgagccaag cctgatgcga
    21601 cgcttgccgc gtcttatcag gcctacaaat gctcccgtag gccagataag gcgtttacgc
    21661 cgcatccggc aacagtgccg gtcagacagc gattaccatt tcactgtcat cgacaaaaac
    21721 cctgccgtct gggcaaaatc atcactccct ttctgccacg ccacgctacc acgcagcgaa
    21781 actcgctgac tgatattgcc cgttattccc acttttattt caccccgttg cttcaccgca
    21841 tcgtcactga tagtgctgcc atcttcttca atttccgtcg catggggatc gtgataataa
    21901 ttcagatcta atgttggtat gacaccaacc gcggtacgcc attcgctgtg taaacccagc
    21961 cgcgtttgaa tatcatcacc ctgcgattgt gacacccgcg catggttagc ggcggtaaaa
    22021 tcatcctgct gcacgccctg ataaatcacc tgcgcctgtg gttcaatcac cactccatgc
    22081 cccggtaacc actgataccc ggcttccagc gaggcgataa tccccgatga gtggtaatga
    22141 tccgcgccat cttcatgttc ggaaacatca ttgttaaacc acgcgtattg cagccagcta
    22201 tccagccagg ccccctgctt ctgattaccg tgctggaacc agcttgaggt cagaccaacg
    22261 gcataaccgt ggttctggtt atcggcgcga gttccggtca tattcgagcg gctgtcgccc
    22321 tggttatcac tgtagccacc aaccgcccca agcatccact cgccatccat gccccagcgc
    22381 ccgctaaaca agtcgccgct aagctgcacc gtagaagtgt cttcatgctg agccagttgc
    22441 cccgctgctg tgtaatgata atctccgccg ataacacgta aattcagcgt ctgaccatcg
    22501 ccaccagcgt gatcgcgtcg ctccatcaca aatgcctgat ttgccgcacg taggttattg
    22561 agatagccac caactttggc attcagcacc ggctggtagg caggcgtagg ggctggagcc
    22621 gggtcgggtg ttggatcagg atccggcgta ggatcaggat ctggagtcgg gtctggatca
    22681 ggtggcgatg gtggcgttac ttcttgcgat cgcagatacc agtcgttgtt atcttccacc
    22741 agcgtgtagt catacgctcc catattgacg tagccgctgc ccgccagact gaactgcgca
    22801 ttgttttgaa attgcgttgg atcggctgcg aaatcaacca ctttaatgcc cgtagatgtc
    22861 ggctcaccaa tacccgtaat gggattaatt accacggtcg tgttgccagc agtattaccg
    22921 ttcaacacca gttggtcgct tgccgagtca tcgccgttta attcgctgtc gagaaacaac
    22981 gtaccgccac cggtgtaatc accgtttacc gtgagcgtgt cgccagcaac gccattttgc
    23041 agactgacag caccggaagt attagttaga tcaccattga ccgtcacgtc tgaaagataa
    23101 gtcaggctgt cagtttcaag gttggtatta agctcgcctg tcagcgtcac accatcggtg
    23161 gcattcacca acgtaccgtc gccgctaaac agcgaggcat ccagcgcctg gttcgtatcc
    23221 tgctgcgtca atcgcaacac agtaccatcg gttatgtcga tatcgccgcc agaggtggca
    23281 tcgattgaac gaatatcctg atttttcccg gtttcaaaaa caccgtcttc ccccacccac
    23341 agtgaagaaa cataaggaat aatattctcc gcttcacctt ttagcgttcc gtcctccaca
    23401 cgtaccgcag acgtcgcagt gccgctggcg gtcaactcca gcgtcccgcc gccactttta
    23461 gtcaacgtgc ttacgctatc cagttgttgc ccggtactat ccgccatcaa cccgccccat
    23521 tgcgtatcta cgccatcctc aacgtgtatt tcgccatcag cgcgcatttc aatattcctg
    23581 ccctgacctg ttgtagccga gcgagtgtca acatcagtcg tcacatccat ttcactgtgt
    23641 tgttggggat ctgtgaagat cactgaacgg ttgtaacttg tgtcccccaa ttgcgaatta
    23701 tcgctgactt caagcgtacc ggaggcgatt tgcgtattac cgagataacc gttgttattc
    23761 gccagcgtta cgcgctgatc ccccgcatta atgacgacat aaacgccatt ctggctggca
    23821 tcactgccgc caatatgtaa atcatcgcca ctgctgatat caacgacatc attactgcca
    23881 atcactgtcc ccccactgcc gctgatttgc agaccgtcat tgtatgagct atcgccatcc
    23941 caagtggtga aatcagagag atccaatact ccgccattta gcacgatcga ttgcggatcg
    24001 ctctgcattg cccgtaaatc ggcctgattc cccgctagcg acaatacggc gttatcttcc
    24061 accacaatat cgccggttaa tgccatcgac tgctcccctg tcaggagata gctgccatct
    24121 tgcgctaccg tcaactgtcc ttcaccctga atcgagccgg aaaaaccgcc ctggtttacg
    24181 gtaacattgc cgcctgcgtc gatattcaaa atgccattag caaaaccggt taatgagtgc
    24241 acaaacgttt gttgtgtatt gccaacattc agttctgcct gatactcaga atgaacagtg
    24301 ctgccgacca tcagaccaaa gcaatcctgc ggatcgctct gacagtgcgt gtcgccgaca
    24361 ttcattaact catcgctgcg ccccagcgtc acctcgccat tctggatact catctcgccg
    24421 gtaaacgcat tattgtcggc attgaggact aactctcccg ctccctcttt cacgatgact
    24481 cccgttccgg caagagaatc aatcgcgcca tcgttgctgg tattgcctat taccagcgtt
    24541 ttatcggcgg caatgttaaa ttcagccaca ctatgaccga tatacataaa gccccccgcc
    24601 gctgccgtcg ccgaatggtc atatcccgag gcgctgttat tttcatcaat catcacacca
    24661 ttgttttggt cataattatc atcaacagaa atatcaataa gatacgggga tgtggtgctg
    24721 ctggtataaa ttgctccgcc ataaccttct gcatagttat tggtaaagga ggtattatta
    24781 attatcgttt ttcctgaaag atgtgtgtta tccgtactat tatccgtaac atcaatggca
    24841 ccgccgtccc catcactata gctggttgat gtatatgcct gattattatc aaaaatgaca
    24901 tcacttaaat aaatatcatt attgatagaa taaatagcgc cacctttacc atcattggcg
    24961 atattattcg caaatattgc attagtgata tttaaatcca tatcaccggc atcgtttgtt
    25021 cccgcagaat agattgctcc gccatagcct cctgcaacgt tactgtcgaa aataacattc
    25081 gcaagattta tcgttgagtt ttctttagca aagatagctc cgccattatt atattcgcca
    25141 ctaatcgagt tattagcaaa cagggtcatc cctgtttcat tttgtggtga tatcgtaaat
    25201 tcagcacttt gctgcaaaaa tacggcacca ccgctggcat tattggccat acccgaaaaa
    25261 atcacccatt gcccatcggc gatgctccag tcctgcgtaa tgcctgaaag gctttgcctg
    25321 ctggcctgac aactcgcttt gatatcatat ccctggcatg aatccaaaac ctccgcgaca
    25381 ctgttaacag agaaaagaga tgcaatcatt gacgggagta aagataaata ctccttgcgt
    25441 agaaaaataa tccgcatatt aatctgacca tcattttcta taagacggca tatttaatcg
    25501 cattatacga tatgggttat ttttcttttg taaattgttc aacccggtgc aatggctatt
    25561 ttgttattat ttcatgaata atatcagtgc gtcataattc aagttaatca ccttaaggga
    25621 tatcagttat gtttaaactg aattaaagta atgaataatt ttcttataat ataaggtaaa
    25681 ttaacaaaat ggcttcgcat ttaacaacaa ccgaagagaa aacaaccatt tcgacatcaa
    25741 caatcgctta cattcgctta tatattgacc ataactgata catcagatta tgtgatgact
    25801 cgtgcttaga tcaatttttg caatcattag caaaaagatt aataaaccat ctatatcaat
    25861 ttatctaacc tattcttccg ttcaagaaat cgccgaacag ttatttttaa caaatttttc
    25921 tcttcccatt gactttcccg gacaccttgt ctgacctaag gtgcgcgaaa gccacttttt
    25981 ccttcctgag ttatccacaa agttatgcac ttgcaagagg gccattttca cactatcttg
    26041 cagtgaatcc caaacatacc ccctatatat agtgttctaa gcagcttccc tcactacagg
    26101 tagtctgcat gaaactattg cggaaagaat tccaaaaaca ggtacgacat acatgaatca
    26161 gaatctgctg gtgacaaagc gcgacggtag cacagagcgc atcaatctcg acaaaatcca
    26221 tcgcgttctg gattgggcgg cagaaggact gcataacgtt tcgatttccc aggtcgaact
    26281 gcgctcccac attcagtttt atgacggtat caagacctct gacatccacg aaaccattat
    26341 caaggctgcc gcagacctga tctcccgtga tgcgccggat tatcagtatc tcgccgcgcg
    26401 cctggcgatc ttccacctgc gtaaaaaagc ctacggccag tttgagccgc ctgcgctgta
    26461 cgaccacgtg gtgaaaatgg tcgagatggg caaatacgat aatcatctgc tggaagacta
    26521 cacggaagaa gagttcaagc agatggacac gtttatcgat cacgaccgtg atatgacctt
    26581 ctcttatgct gccgttaagc agctggaagg caaatatctg gtgcagaacc gtgtgaccgg
    26641 cgaaatctac gaaagcgccc agttccttta tattctggtt gccgcgtgcc tgttctcgaa
    26701 ctacccgcgt gaaacgcgcc tgcaatacgt gaagcgtttt tacgacgcgg tttccacatt
    26761 taaaatttcg ctgccgacgc caatcatgtc cggcgtgcgt accccgactc gtcagttcag
    26821 ctcctgcgta ctgatcgagt gcggcgacag cctggattcc atcaacgcca cctccagcgc
    26881 gattgttaaa tacgtttccc agcgtgccgg gatcggcatc aacgccgggc gtattcgtgc
    26941 gctgggtagc ccgattcgcg gtggcgaagc gttccatacc ggttgtattc cgttctataa
    27001 gcatttccag acagcggtga aatcctgctc tcagggcggt gtgcgcggcg gtgcggcaac
    27061 actgttctac ccgatgtggc atctggaagt tgaaagcctg ctggtgttga aaaacaaccg
    27121 tggggtggaa ggcaaccgcg tgcgtcatat ggactacggg gtacaaatca acaaactgat
    27181 gtatacccgt ctgctgaaag gtgaagatat cactctgttc agcccgtccg acgtaccggg
    27241 gctgtacgac gcgttcttcg ccgatcagga agagtttgaa cgcctgtaca ccaaatatga
    27301 gaaagatgac agcatccgca agcagcgtgt gaaagccgtt gagctgttct cgctgatgat
    27361 gcaggaacgt gcgtctaccg gtcgtatcta tattcagaac gtagaccact gcaacaccca
    27421 cagcccgttt gatccggcta ttgcaccagt gcgccagtct aacctgtgcc tggaaatcgc
    27481 cctaccgacc aaaccactga acgacgtcaa cgacgagaac ggtgaaatcg cgctgtgtac
    27541 gctgtctgct ttcaacctgg gcgcaattaa cagcctggat gaactggaag agctggcaat
    27601 tctggcggtt cgcgcactcg acgcactgct ggattatcag gattacccga tcccggccgc
    27661 taaacgtgga gcgatggggc gtcgtacgct gggtattggt gtgatcaact tcgcttacta
    27721 cctggcgaag cacggtaaac gctactccga cggcagcgcc aacaacctga cgcataaaac
    27781 cttcgaagcc attcagtatt acctgctgaa agcgtctaat gagctggcga aagagcaagg
    27841 cgcgtgcccg tggtttaacg aaaccactta cgcgaaaggg atcctgccga tcgataccta
    27901 taagaaagat ctggatgcca tcgctaatga gccgctgcat tacgactggg aagcactgcg
    27961 tgagtcaatc aaaacgcacg gcctgcgtaa ctccacgctt tctgctctga tgccgtcaga
    28021 gacctcttcg cagatctcta acgccactaa cggtattgaa ccgccgcgtg gttacgtcag
    28081 catcaaagcg tcgaaagacg gtattctgcg ccaggtggtg ccggactacg agcatctgca
    28141 cgacgcctat gaactgctgt gggaaatgcc gggtaacgat ggctatctgc aactggtggg
    28201 tatcatgcag aaatttatcg atcagtcgat ctctgctaac accaactacg atccgtcacg
    28261 cttcccgtca ggaaaagtgc cgatgcagca actgctgaaa gacctgctca ccgcctacaa
    28321 attcggggtt aaaacgctgt attatcagaa cacccgtgac ggtgcagaag acgcacaaga
    28381 cgatctggtg ccgtcaatcc aggacgatgg ctgcgaaagc ggcgcatgta agatctgata
    28441 ttgagatgcc ggatgcggcg tgaacgcctt atccggcata cggctcggtt tgtaggcctg
    28501 ataagacgcg ccagcgtcgc atcaggcaca ggatgcggcg taaaatgcct tatccggcat
    28561 taaactccca acaggacaca ttcatggcat ataccacctt ttcacagacg aaaaatgatc
    28621 agctgaaaga accgatgttc tttggtcagc cggttaacgt ggctcgctac gatcagcaaa
    28681 aatatgacat cttcgaaaag ctgatcgaaa agcagctctc tttcttctgg cgtccggaag
    28741 aagttgacgt ttctcgtgac cgtatcgatt accaggcact acctgaacac gaaaaacaca
    28801 tctttatcag caacctgaaa tatcagacgc tgctggactc cattcagggt cgcagcccga
    28861 acgtggcact gctgccgctg atctcgatcc cggaactgga aacctgggta gaaacctggg
    28921 cgttctcaga aacgattcat tcccgctcct atacccatat catccgtaat atcgttaacg
    28981 atccgtcagt tgtctttgac gatatcgtca ccaacgagca gatccagaaa cgtgcggaag
    29041 ggatctccag ctattacgat gaactgatcg aaatgaccag ctactggcat ctgctgggcg
    29101 aaggtaccca caccgttaac ggtaaaaccg taaccgttag cctgcgcgag ctgaagaaaa
    29161 aactgtatct ctgcctgatg agcgttaacg cgctggaagc gatccgcttc tacgtcagct
    29221 ttgcttgttc ctttgccttt gcagaacgcg aactgatgga aggtaacgcc aaaattattc
    29281 gcctgattgc ccgcgacgaa gccctgcacc tgaccggcac ccagcatatg ctgaatctgc
    29341 tgcgcagcgg cgcggatgat ccggagatgg cggaaattgc cgaagaatgt aagcaggagt
    29401 gctatgacct gtttgtccag gcggcacagc aagagaaaga ctgggcggat tatctgttcc
    29461 gtgatggttc gatgattggt ctgaataaag acatcctctg ccagtacgtt gaatacatca
    29521 ccaatatccg tatgcaggca gtcggtttgg atctgccgtt ccagacgcgc tccaacccga
    29581 tcccgtggat caacacttgg ttggtgtctg ataacgtaca ggttgctccg caggaagtgg
    29641 aagtgagttc ttatctggtc gggcagattg acgcggaagt ggacaccgac gatttgagta
    29701 acttccagct ctgatggccc gcgttaccct gcgcatcact ggcacacagc tgctgtgcca
    29761 ggatgaacac ctttcccttc tggcggcgct ggaatcccac aatgtggcgg ttgagtatca
    29821 gtgccgcgaa ggttactgcg gttcctgtcg cacacgcctt gttgcaggcc aggtggactg
    29881 gattgccgaa ccgttagcct ttattcagcc gggggaaatt ttgccctgtt gttgccgggc
    29941 aaaaggcgat attgaaatcg agttgtgatc gggtactagc gccagatacg acgcttacgc
    30001 gtattatctg gcctacaacg attacatggc gtagtaatac gctttcacct gctcccagtc
    30061 cgctttaggg atcggctcca gatatttttc cagctggcgg aagtcatgat taatcgcttt
    30121 atcacgacgc aagcggcgac gacttttctc caggtcaaga aatccagctt ctgccttacc
    30181 ttctgttttc acatagatat ggcgaacata acaacagcca tgctgacgat taacgctatg
    30241 cattttctta aacgccagcg ccactgcttt caacatggct tgccgtactt cgtcagaata
    30301 aggcgatact gcatgccggg cataccagtc agcaatgctg atgaaccccg ccatatcttc
    30361 agtcaccagc aacgcgcgcc attccccctc aattttcacc gcttcaccaa aaacgatctt
    30421 cggcacgatg acaccagccc gttccagttc tttaattacc gcaacctcac ggacaatcgt
    30481 tggtcgaccg aacggataac gtacggaatg aaacagatga tgcgtcatgc gctttacata
    30541 tagctttttg ccgttgcgct cgacgcattg caccccgctc ataccattac ggcgatagtt
    30601 aggctcttca acccagtcgc cctctgttgc ccaccagtga ttaaattcgt cgtgctttgc
    30661 tgaaactgcc ataccctatc gcctgtcatt ttttatttac gacaatgact ataggtggtt
    30721 acctgcgaaa aatcttaatg aaacgtgtcg tattaatgaa tttcttttgc cgctactagc
    30781 atgacaccgc ttttgccctt catttgcagc tcatagactc caccagccac taccactaat
    30841 agtgcaccca gcagataaat cattcggtag ccagtatgtg caaccatcat ccccatcaca
    30901 caggctccca gcgccaggcc tgcatccatc gcattgaaga agagggagtt tgcgacaccg
    30961 attttatgcg gttccactga actgataatc tgcgtctgaa aaactggcgt cacggaacca
    31021 tacccgatac caatcaacgc gccagaaaca accatcatta tgcttccctg ggtgtagccg
    31081 agaaccacca gtccaaccgt aaagaacaca aggcaagggt aaaccacata tttcggtcct
    31141 tttttgtcgc agacattgcc ggtaaaggta cggcagatca tcaggaagat agcgtagcaa
    31201 agca
//
LOCUS       DAMZFT010000051        30502 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.051, whole genome shotgun sequence.
ACCESSION   DAMZFT010000051 DAMZFT010000000
VERSION     DAMZFT010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 30502)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30502)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30502
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.051"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(<1..598)
                     /gene="rclR"
                     /locus_tag="PN977_002382"
     CDS             complement(<1..598)
                     /gene="rclR"
                     /locus_tag="PN977_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RclR"
                     /protein_id="HDI6042408.1"
                     /translation="MDALSRLLMLNAPQGTIDKNCVLGSDWQLPHGAGELSVIRWHAL
                     TQGAAKLEMPTGEIFTLRPGNVVLLPQNSAHRLSHVDNESTCIVCGTLRLQHSARYFL
                     TSLPETLFVAPVNHSVEYNWLREAIPFLQQESRSAMPGVDALCSQICATFFTLAVREW
                     IAQVNTEKNILSLLLHPRLGAVIQQMLEMPGHAWTVESL"
     gene            825..2150
                     /locus_tag="PN977_002383"
     CDS             825..2150
                     /locus_tag="PN977_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridine nucleotide-disulfide oxidoreductase"
                     /protein_id="HDI6042409.1"
                     /translation="MNKYQAVIIGFGKAGKTLAVTLAKAGWRVALIEQSNAMYGGTCI
                     NIGCIPTKTLVHDAQQHTDFVRAIQRKNEVVNFLRNKNFHNLADMPNIDVIDGQAEFI
                     NNHSLRVHRPEGNQEIHGEKIFINTGAQTVVPPIPGITTTPGVYDSTGLLNLKELPAH
                     LGILGGGYIGVEFASIFANFGSKVTILEAASLFLPREDRDIADNIATILRDQGVDIIL
                     NAHVERISHHENQVQVHSEHAQLAVDALLIASGRQPATASLHPENAGIAVNERGAIVV
                     DKQLHTTANNIWAMGDVTGGLQFTYISLDDYRIVRDELLGEGRRSTDDRKNVPYSVFM
                     TPPLSRVGMTEEQARESGADIQVVTLPVAAIPRARVMNDTRGVLKAIVDNKTQRILGA
                     SLLCVDSHEMINIVKMVMDAGLPYSILRDQIFTHPSMSESLNDLFSLVK"
     gene            2259..2495
                     /locus_tag="PN977_002384"
     CDS             2259..2495
                     /locus_tag="PN977_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HDI6042410.1"
                     /translation="MFKKSVLFATLLSGVMAFSTNADDKIILKHISVSSVSASPTVLE
                     DAIADMARKYNASSWKVTSMRIDNNSTATAVLYK"
     gene            2507..3100
                     /gene="rclC"
                     /locus_tag="PN977_002385"
     CDS             2507..3100
                     /gene="rclC"
                     /locus_tag="PN977_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reactive chlorine species resistance protein
                     RclC"
                     /protein_id="HDI6042411.1"
                     /translation="MEKYLHLLSRGDKIGLTLIRLSIAIVFMWIGLLKFVPYEADSIT
                     PFVANSPLMSFFYEHPEDYKQYLTHEGEYKPEARAWQTANNTYGFSNGLGVVEVIIAL
                     LVLANPVNRWLGLLGGLMAFTTPLVTLSFLITTPEAWVPALGDAHHGFPYLSGAGRLV
                     LKDTLMLAGAVMIMADSARDILKQRSNESSSTLKTEY"
     gene            complement(3262..4131)
                     /locus_tag="PN977_002386"
     CDS             complement(3262..4131)
                     /locus_tag="PN977_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001571002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HDI6042412.1"
                     /translation="MEFSVLSNNLKMPMMGFGVFQVTDKDVCKQSVLNAIRTGYRLID
                     TAAVYGNEDAVGEAVREAISEGLCTREELFITSKLWVQDMLNQDIAAAGIEASLKKSG
                     LEYFDLYLLHQAMRDYFSAWRALEDAYEEGKLKAIGVSNFYPHVLANFCETVRVKPMV
                     NQVELHPYFAQPEALATMKYYNVQPEAWAPLGGGRHKPFENNLLQSIADAHQKSISQV
                     ILRWNIQRGVVVIPKSTHQQRIEENFAIWDFSLTEKEMAQISSLDLGYVGESVKHFNP
                     EFVRGCLAVKTHG"
     gene            4379..5236
                     /locus_tag="PN977_002387"
     CDS             4379..5236
                     /locus_tag="PN977_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308364.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDI6042413.1"
                     /translation="MIRQKILQQLLEWIECNLEHPISIEDIAQKSGYSRRNIQLLFRN
                     FMHVPLGEYIRKRRLCRAAILVRLSAKSMLDIALSLHFDSQQSFSREFKKLFGCSPRE
                     YRHRDYWDLANIFPSFLIRQQQKTECRLVNFPETPIFGNSFKYYIEVSNKSPDEEVKL
                     RRHHLVRCMKNFKTDIYFVSTFEPSTKSVDLLTVETFAGTVCKETDSEMSKEWTINRG
                     LYASFRYEGEWELYPEWTRNLYLMELPARRLARMNGSDIERFYYNEDFVEKDSNDVVC
                     EIFIPVRPV"
     gene            complement(5357..9611)
                     /gene="fdeC"
                     /locus_tag="PN977_002388"
                     /pseudo
     CDS             complement(5357..9611)
                     /gene="fdeC"
                     /locus_tag="PN977_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000092554.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="intimin-like adhesin FdeC"
     gene            10177..11028
                     /locus_tag="PN977_002389"
     CDS             10177..11028
                     /locus_tag="PN977_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001195265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HDI6042414.1"
                     /translation="MQKVKLNNGIEMPLLGFGVFQMTDAAECERAVIDAINSGYRLID
                     TAASYQNEIQVGNALKQSGIARNELFVTTKLWLQDTSYEGAKAQFERSLNRLQLDYVD
                     LYLIHQPYGDVHGAWRAMEELQQAGKIRAIGVSNFHPDRLADLIAFNNVVPAVNQVEV
                     NPFNQQLQAVPWMQSRGIQPEAWAPFAEGKNGLFQHPVLTAIGEKYGKSVGQVVLRWI
                     YQRGIVSLAKSVRKERMEENINILDFELSPEDMLQITALDTATSAFFSHRDPAMVEWL
                     TGRKLDV"
     gene            11055..12044
                     /locus_tag="PN977_002390"
     CDS             11055..12044
                     /locus_tag="PN977_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001172283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HDI6042415.1"
                     /translation="MQKRYLGKSGLEVSALGLGCMGLSHGYGPATDTRQAIELIRAAV
                     ERGVTFFDTAEVYGPYLNEEVVGEALKPFRDRVVIATKFGFTFGDDNKQQILNSRPEH
                     IREAVEGSLRRLKTDVIDLLYQHRVDPDVPIEDVAGTVKDLIAEGKVKHFGLSEAGAQ
                     TIRRAHAVQPVTALQSEYSMWWREPEQEILPLLEELGIGFVPFSPLGKGFLTGAIKPG
                     TTFGKDDYRSTVPRFAAQAIEANEKLVTLLGELAAEKGVTSAQIALAWLLAQKPWIVP
                     IPGTTKLNRLEENLAAADIVLSQKDTQQITEALETIKIVGERYSPEHQARVGR"
     gene            complement(12075..12968)
                     /locus_tag="PN977_002391"
     CDS             complement(12075..12968)
                     /locus_tag="PN977_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016234625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDI6042416.1"
                     /translation="MLKENFNELQIFLVVARERSFTKAAGKLGVSQSALSHAMKALEE
                     RLNIRLLTRTTRSVAPTEAGERIIACLEPRIDELEQELESLIQLNSTPSGNIRLSAGE
                     HAARSLVWPKLKPFLREYPEINVELVVDNGFVDIVEGRFDAGIRLGENVDKDMVAVRI
                     GPDMRMAVVGAPAYFAANPAPETPHELQNHRCINMRLPTAGGLYHWEFEREGKPLRVK
                     VDGQLTCSPLPERIDAALSGFGIACVPEDMVQEYIKSGKLIQVLQEWCPTFPGYYLYY
                     PSRKQHPPAFALLIDALRYTE"
     gene            complement(13168..14094)
                     /locus_tag="PN977_002392"
     CDS             complement(13168..14094)
                     /locus_tag="PN977_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDI6042417.1"
                     /translation="MMKIEPSILPSLAWFALIVRAGSFSRAASEMGITRAALSQNLKS
                     LEERLNTKLIYRTTRNMSLTEEGQRLYEVLVSALGQIDDALKDVGDTQLEPTGLLRIN
                     SSRVAARMLVEPHIGEFLTRYPKTKIELIMDDGLSNIIADGCDVGIRLEQGLDEHMTA
                     VPVSPLIKLVTVASPDYLKEHGIPETPQELSNHNCLRLRHKSSGALSAWEFSNVVGGN
                     EEFEIEVSGKYISNDDESMIRMALNGTGIIQHLDFAIAEHINAGKLQPILEDWAVSFP
                     GFYIYVSSRVRMPSKVRAFIDFMVEKRVKIES"
     gene            14251..15171
                     /locus_tag="PN977_002393"
     CDS             14251..15171
                     /locus_tag="PN977_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDI6042418.1"
                     /translation="MKTVSIKHTYWDIAADIYFPDDFNSEKKYPAIISAHPIGSCKEQ
                     TSGSVYGAALAKAGFIVIAFDASFQGSSGGEPRYLEDPTMRVKDFSIVVDYLTTLPYV
                     DAGRIGVLGICGGGGYAINAAMTERRIKAIGTVTGANYGRLMREGFTAFNPIGALEAM
                     AQQRTDEANGAKLRVDDLLPSSPEAALEAGLTEIDLFEATEYYRSPRGCAPNGVNRSL
                     FSHQTVAVGWDAFHLAEVLLTQPLMVVVGDRVGAFGAYRDGCEIIGRAASKHKELVVV
                     EGYSHYDLYDKPEPVKQALDKLIPFYKTHL"
     gene            15416..16558
                     /locus_tag="PN977_002394"
     CDS             15416..16558
                     /locus_tag="PN977_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HDI6042419.1"
                     /translation="MTNKHSSLFSPFMLTEKIKLRNRIVMAPMTTWSANPDGTISEQE
                     LEFYKRRSQNVGLVITGCTYVTPSGIGFTHEFAAYDDRFINSLEKLAAAAKSGGAPAI
                     LQIFHAGNKAIPELVPNNDVISASASSVKSGDFMKRVVQSREMTENEIQETIRAFGDV
                     TKRAIKAGFDGVELHGAHGFLLQNFFSPLFNQRNDRWGGDLEGRMRFPLAVLQEVKNV
                     VYEYATKPFAIGYRISPEESVTGGLRIEDTYKLLDRLISSGISYIHTSLVSINDSYPV
                     ESPNGPRTIELILNHIAGRVPVIAAGKIRTPSQAQEAISAGLPLVAIGKGLVINPEWV
                     TLAESGRSHEIQTALNPQRVPELTIPDKLWDQIQASKGTGWFPLID"
     gene            17032..17133
                     /locus_tag="PN977_002395"
     CDS             17032..17133
                     /locus_tag="PN977_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6042420.1"
                     /translation="MKENKVQQISHKLINIVVFVAIVEYAYLFLHFY"
     gene            17498..17761
                     /locus_tag="PN977_002396"
     CDS             17498..17761
                     /locus_tag="PN977_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HDI6042421.1"
                     /translation="MKPNIHPEYRTVVFHDTSVDEYFKIGSTIKTDREIELDGVTYPY
                     VTIDVSSKSHPFYTGKLRTVASEGNVARFTQRFGRFVSTKKGA"
     gene            17761..17901
                     /gene="ykgO"
                     /locus_tag="PN977_002397"
     CDS             17761..17901
                     /gene="ykgO"
                     /locus_tag="PN977_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L36"
                     /protein_id="HDI6042422.1"
                     /translation="MKVLNSLRTAKERHPDCQIVKRKGRLYVICKSNPRFKAVQGRKK
                     KR"
     gene            complement(17971..18161)
                     /locus_tag="PN977_002398"
                     /pseudo
     CDS             complement(17971..18161)
                     /locus_tag="PN977_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308353.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            18985..19527
                     /gene="ecpR"
                     /locus_tag="PN977_002399"
                     /gene_synonym="matA"
     CDS             18985..19527
                     /gene="ecpR"
                     /locus_tag="PN977_002399"
                     /gene_synonym="matA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECP biosynthesis operon DNA-binding
                     transcriptional regulator EcpR"
                     /protein_id="HDI6042423.1"
                     /translation="MECQNRSDKYIWSPHDAYFYKGLSELIVDIDRLIYLSLEKIRKD
                     FVFINLNTDSLTEFINRDNEWLSAVKGKQVVLIAARKSEALANYWYYNSNIRGVVYAG
                     LSRDIRKELAYVINGRFLRKDIKKDKITDREMEIIRMTAQGMLPKSIARIENCSVKTV
                     YTHRRNAEAKLYSKLYKLVQ"
     gene            19602..20189
                     /gene="ecpA"
                     /locus_tag="PN977_002400"
                     /gene_synonym="matB"
     CDS             19602..20189
                     /gene="ecpA"
                     /locus_tag="PN977_002400"
                     /gene_synonym="matB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="common pilus major fimbrillin subunit EcpA"
                     /protein_id="HDI6042424.1"
                     /translation="MKKKVLAIALVTVFTGTGVAQAADVTAQAVATWSATAKKDTTSK
                     LVVTPLGSLAFQYAEGIKGFNSQKGLFDVAIEGDSTATAFKLTSRLITNTLTQLDTSG
                     STLNVGVDYNGAAVEKTGDTVMIDTANGVLGGNLSPLANGYNVSNRTTAQDGFTFSII
                     SGTTNGTTAVTDYSTLPEGIWSGDVSVQFDATWTS"
     gene            20247..20915
                     /gene="ecpB"
                     /locus_tag="PN977_002401"
                     /gene_synonym="yagY"
     CDS             20247..20915
                     /gene="ecpB"
                     /locus_tag="PN977_002401"
                     /gene_synonym="yagY"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone EcpB"
                     /protein_id="HDI6042425.1"
                     /translation="MKKHLLPLALLLSGISPAQALDVGDISSFMNSDSSTLSKTIKNS
                     TDSGRLINIRLERLSSPLDDGQVISMDKPDELLLTPASLLLPAQASEVIRFFYKGPAD
                     EKERYYRIVWFDQALSDAQRDNANRSAVATASARIGTILVVAPRQANYHFQYANGSLT
                     NTGNATLRILAYGPCLKAANGKECKENYYLMPGKSRRFTRVDTADNKGRVALWQGDKF
                     IPVK"
     gene            20941..23466
                     /gene="ecpC"
                     /locus_tag="PN977_002402"
                     /gene_synonym="yagX"
     CDS             20941..23466
                     /gene="ecpC"
                     /locus_tag="PN977_002402"
                     /gene_synonym="yagX"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher EcpC"
                     /protein_id="HDI6042426.1"
                     /translation="MPLRRFSPGLKAQFAFGMVFLFVQPDASAADISAQQIGGVIIPQ
                     AFSQALQDGMSVPLYIHLAGSQGRQDDQRIGSAFIWLDDGQLRIRKIQLEESEDNASV
                     SEQTRQQLMTLANAPFNEALTIPLTDNAQLDLSLRQLLLQLVVKREALGTVLRSRSED
                     IGQSSVNTLSSNLSYNFGVYNNQLRNGGSNTSSYLSLNNVTALREHHVVLDGSLYGIG
                     SGQQDSELYKAMYERDFAGHRFAGGMLDTWNLQSLGPMTAISAGKIYGLSWGNQASST
                     IFDSSQSATPVIAFLPAAGEVHLTRDGRLLSVQNFTMGNHEVDTRGLPYGIYDVEVEV
                     IVNGRVISKRTQRVNKLFSRGRGVGAPLAWQIWGGSFHMDRWSENGKKTRPAKESWLA
                     GASTSGSLSTLSWAATGYGYDNQAVGETRLTLPLGGAINVNLQNMLASDSSWSNIASI
                     SATLPGGFSSLWVNQEKTRIGNQLRRSDADNRAIGGTLNLNSLWSKLGTFSISYNDDR
                     RYNSHYYTADYYQNVYSGTFGSLGLRAGIQRYNNGDSSANTGKYIALDLSLPLGNWFS
                     AGMTHQNGYTMANLSARKQFDEGTIRTVGANLSRAISGDTGDDKTLSGGAYAQFDARY
                     ASGTLNVNSAADGYINTNLTANGSVGWQGKNIAASGRTDGNAGVIFNTGLEDDGQISA
                     KINGRIFPLNGKRNYLPLSPYGRYEVELQNSKNSLDSYDIVSGRKSHLTLYPGNVAVI
                     EPEVKQMVTVSGRIRAEDGTLLANARINNHIGRTRTDENGEFVMDVDKKYPTIDFRYS
                     GNKTCEVALELNQARGAVWVGDVVCSGLSSWAAVTQTGEENES"
     gene            23456..25099
                     /gene="ecpD"
                     /locus_tag="PN977_002403"
     CDS             23456..25099
                     /gene="ecpD"
                     /locus_tag="PN977_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial adhesin EcpD"
                     /protein_id="HDI6042427.1"
                     /translation="MRVNLLIAMIIFALIWPATALRAAVSKTTWADAPAREFVFVENN
                     SDDNFFVTPGGALDPRLTGANRWTGLKYNGSGTIYQQSLGYIDNGYNTGLYTNWKFDM
                     WLENSPVSSPLTGLRCINWYAGCNMTTSLILPQTTDASGFYGATVTSGGAKWMHGMLS
                     DAFYQYLQQMPVGSSFTMIINACQTSVNYDASSGARCKDQASGNWYVRNVTHTKAANL
                     RLINTHSLAEVFINSDGVPTLGEGNADCRTQTIGSRSGLSCKMVNYTLQTNGLSNTSI
                     HIFPAIANSSLASAVGAYDMQFSLNGSSWKPVSNTAYYYTFNEMKSADSIYVFFSSNF
                     FKQMVNLGISDINTKDLFNFRFQNTTSPESGWYEFSTSNTLIIKPRDFSISIISDEYT
                     QTPSREGYVGSGESALDFGYIVTTSGKTAADEVLIKVTGPAQVIGGRSYCVFSSDDGK
                     AKVPFPATLSFITRNGATKTYDAGCDDSWRDMTDALWLTTPWTDISGEVGQMDKTTVK
                     FSIPMDNAISLRTVDDNGWFGEVSASGEIHVQATWRNIN"
     gene            25068..25778
                     /gene="ecpE"
                     /locus_tag="PN977_002404"
     CDS             25068..25778
                     /gene="ecpE"
                     /locus_tag="PN977_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308346.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone EcpE"
                     /protein_id="HDI6042428.1"
                     /translation="MFRRRGVTLTKALLTVVCMLAAPLTQAISVGNLTFSLPSETDFV
                     SKRVVNNNKSARIYRIAISAIDSPGSSELRTRPVDGELLFAPRQLALQAGESEYFKFY
                     YHGPQDNRERYYRVSFREVPTRNQTRRSPTGGEVSTEPVVVMDTILVVRPRQVQFKWS
                     FDQVTGTVSNTGNTWFKLLIKPGCDSTEEEGDAWYLRPGDVVHQPELRQPGNHYLVYN
                     DKFIKISDSCPAKPPSAD"
     gene            26091..26420
                     /locus_tag="PN977_002405"
     CDS             26091..26420
                     /locus_tag="PN977_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HDI6042429.1"
                     /translation="MSNLNPCMTCGACCAFFRVSFYWAEADDAGGTIPARLTEQISPF
                     HRCMSGTNQKNPRCIALAGTPGKNACCTIYKNRSSTCREFAMSGENGVVNEACNRARA
                     KYGLTPL"
     gene            complement(26669..27283)
                     /locus_tag="PN977_002406"
     CDS             complement(26669..27283)
                     /locus_tag="PN977_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YagU family protein"
                     /protein_id="HDI6042430.1"
                     /translation="MNIFEQTPPNRRRYGLAAFIGLIAGVVSAFVKWGAEVPLPPRSP
                     VDMFNAACGPESLIRAAGQIDCSRNFLNPPYIFLRDWLGLTDPNAAVYTFAGHVFNWV
                     GVTHIIFSIVFAVGYCVVAEVFPKIKLWQGLLAGALAQLFVHMISFPLMGLTPPLFDL
                     PWYENVSEIFGHLVWFWSIEIIRRDLRNRITHEPDPEIPLGSNR"
     gene            complement(28076..28444)
                     /locus_tag="PN977_002407"
     CDS             complement(28076..28444)
                     /locus_tag="PN977_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001094399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin"
                     /protein_id="HDI6042431.1"
                     /translation="MKTLPATTPQAAKPCLSPVSVWQMLLTRLLEQHYGLMLSDTPFS
                     DETVIKEHIDAGITLANAVNFLVEKYELVRIDRNGFTSQVQAPYLTTTDILQARKACG
                     LMSRCSYREVSNIVLSRSRL"
     gene            complement(28465..28782)
                     /locus_tag="PN977_002408"
     CDS             complement(28465..28782)
                     /locus_tag="PN977_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system YeeU family
                     antitoxin"
                     /protein_id="HDI6042432.1"
                     /translation="MSNITWGLQRDITPRLGARLVQEGNQLHYLADRASITGKFSDAE
                     YRKLDETFPHFISQMESMLTTGEMNPRHAHCVTLYHDGFTCEADTLGSCGYVYIVVYS
                     TQR"
     gene            complement(28802..29023)
                     /locus_tag="PN977_002409"
     CDS             complement(28802..29023)
                     /locus_tag="PN977_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF987 domain-containing protein"
                     /protein_id="HDI6042433.1"
                     /translation="MRIISKRRAMTIYRQHPASRIFRYCTGRYQWHGSVCHYTGLAVP
                     DIPGVLVVYAERRQDRSGPYACLMSITLN"
     gene            complement(29032..29511)
                     /locus_tag="PN977_002410"
     CDS             complement(29032..29511)
                     /locus_tag="PN977_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000438147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RadC family protein"
                     /protein_id="HDI6042434.1"
                     /translation="MEQSLIPLAPALPLTAQRTVKRALTLLDRHLRETGVAFTSTQAA
                     RDWLKLKMAGLEREEFMVLYLNQQNQLIAHETLFAGSISSTEVHPREVVKRALYFNAA
                     AVILAHNHPSGDTSPSKADKTITQRLVQALQLVDIRVPDHLIVGGTQILSFAEHGLL"
     gene            complement(29523..29981)
                     /locus_tag="PN977_002411"
     CDS             complement(29523..29981)
                     /locus_tag="PN977_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HDI6042435.1"
                     /translation="MTTQTQCELMSANEPQFDLTATPVPDEQRLDFWPLHFGAITQWI
                     TLEPRIFAWMDRFCDEYSGGLWSFYTLSNGGAFMAPDAEGGDKWHLFNGMNGNGAEMS
                     AEAAGIAVCLIEYSHHACHTECDAMTEHYYRLRDYSLQHPESSAILRIID"
     gene            complement(30067..30303)
                     /locus_tag="PN977_002412"
                     /pseudo
     CDS             complement(30067..30303)
                     /locus_tag="PN977_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004272.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 domain-containing protein"
BASE COUNT         8177 a         7411 c         7051 g         7863 t
ORIGIN      
        1 ccagcgattc gacggtccag gcgtgtcctg gcatttccag catttgctgt attaccgcac
       61 caagacgtgg atgcagaagc aaactgagaa tgtttttctc tgtattaacc tgtgcaatcc
      121 actcacgcac cgcgagggta aagaatgtag cgcagatctg gctacacaga gcatccactc
      181 ccggcattgc cgatctggat tcctgttgta aaaacgggat cgcctccctc agccagttat
      241 attccacgct atggttcact ggtgctacaa acagcgtttc cggcaaagac gttaaaaaat
      301 aacgcgccga atgttgcagt cgaagagtgc cacagacaat acaggtcgat tcattatcga
      361 catgactcag gcgatgagct gaattttgtg gtagcaggac cacatttccc gggcgtaatg
      421 taaaaatctc tcccgtcggc atttccagct tcgccgctcc ttgcgttaac gcatgccaac
      481 gaataaccga caattccccg gcaccatgtg gaagttgcca gtcacttcct aacacgcaat
      541 tcttatcgat cgttccttgt ggagcgttaa gcatcaacaa acggctaaga gcatccatat
      601 cagactcctg agtggttttt tgattacaaa caccaacaat cttagtcaat aattaatcta
      661 ataccatcaa atagttaatc atgatttttg ttttgtgatt ctgattaaga aaaagaaaat
      721 accagacgat aagacaaaat atcaagcgtt gtgaagaaat gttatttgct cttttgcgtc
      781 tatagtcatg atgtcaaatg aacgcgtttc gacaggaaat catcatgaat aaatatcagg
      841 cagttattat tggttttggc aaggctggaa aaacattagc cgtcacgctg gcaaaagcag
      901 gttggcgtgt ggctctcatc gaacaatcaa atgcaatgta tggcgggacc tgtattaata
      961 tcggctgtat cccaaccaaa acattggttc atgacgcaca gcagcacaca gattttgtcc
     1021 gtgccataca gcgtaaaaat gaagtggtta attttttacg taataagaat tttcataatc
     1081 ttgcggatat gcccaatatc gacgtgatcg acggccaggc ggagtttatc aataatcata
     1141 gcctgcgtgt tcatcggcct gagggaaatc aggagattca tggcgagaaa atttttatta
     1201 ataccggtgc acaaaccgtg gttccgccaa ttcctggaat taccaccacg cctggtgtat
     1261 atgacagcac cggattactt aatctaaaag aattgcctgc acatttaggt attttgggcg
     1321 gcggatatat tggcgttgag ttcgcctcta tattcgctaa ttttggcagc aaagtaacca
     1381 ttttagaggc agcttcgctg tttttgcctc gggaagatcg ggatattgct gataatatcg
     1441 cgacgatttt acgcgatcag ggcgtcgata taatcctcaa tgcccatgtg gagcgaatca
     1501 gtcaccatga aaatcaagtg caagtgcata gcgagcacgc ccaactggcg gtggatgcac
     1561 tgttaatagc ttccggtcgt caaccggcta ccgcttcgtt acatccagaa aatgcaggta
     1621 tcgcagtaaa cgagcgcggg gcaattgtcg ttgacaagca attacatacc accgcaaaca
     1681 atatttgggc gatgggagat gttaccggcg ggctgcaatt tacttacatt tcactggatg
     1741 attaccgcat tgtgcgtgat gaattgctgg gagaaggcag acgtagtact gatgatcgga
     1801 aaaatgtgcc ttattccgta ttcatgacac cgccactgtc cagggttggt atgacagaag
     1861 aacaggccag agagagtggt gctgatattc aggtggtgac attgcctgta gctgcaattc
     1921 cgcgtgccag agtgatgaat gatactcgtg gggtattaaa agcgattgtt gataataaaa
     1981 cccaacgtat attaggggca tcactgctgt gtgttgactc ccacgagatg atcaatatag
     2041 tgaaaatggt gatggatgcc gggctgcctt atagcatatt acgcgatcag atatttactc
     2101 atccgtcgat gagcgaatca ctcaatgatc tattttcatt agtcaaataa actcaaaatc
     2161 agacgccaga acaaatattc tggcgtctca gagaaaagaa tcttattaat tccctgttac
     2221 tttataactt cttaatcact tcattgatgg tattttatat gtttaaaaaa tctgttttat
     2281 ttgcaacact attatctggc gttatggcat tttccaccaa tgcagatgat aaaataattc
     2341 tgaaacatat cagcgtctcg tcagtatcag catcaccgac agttcttgag gatgccattg
     2401 ctgatatggc cagaaaatat aatgcttcat cctggaaagt cacatcgatg cgaattgata
     2461 ataattcaac cgcaacagca gtattgtata aataaggatg tttacaatgg agaaatacct
     2521 gcacctgtta agtcggggag ataaaattgg cctgacattg attcgtctga gtattgcgat
     2581 tgtttttatg tggattgggt tattaaagtt tgtcccttat gaggcagaca gcattacacc
     2641 attcgtcgca aacagtccac taatgtcgtt cttttatgaa cacccagaag actataaaca
     2701 gtatctgact cacgaaggcg aatacaaacc agaagcaaga gcatggcaaa cggctaataa
     2761 tacctatggt ttttccaacg gtcttggcgt cgtggaggtg attattgctc tgctggtttt
     2821 ggctaatcct gtcaatcgct ggttaggttt attgggtggg ctgatggcat ttaccacgcc
     2881 gctggtaaca ctctcatttt taatcaccac tccggaagca tgggtacccg cattgggtga
     2941 tgctcatcat ggtttccctt atttatccgg tgctggtcgt ctggtattga aagatactct
     3001 gatgctggca ggagctgtca tgataatggc agattcggcg cgggacattc ttaaacaacg
     3061 cagtaatgaa tccagttcaa cgttaaaaac tgaatattga taatccgaac actgtctgtt
     3121 gttcactttt tcaggcgtca ggctttcctt gctaatgggt gaagcctgac gccatattac
     3181 ctgaaacagg acaatgtaag gttcaatata actcccttat taaaacattt atgtcatctc
     3241 aggtaaaata agtgattaat atcaaccatg tgtttttaca gcaagacaac cacgaacaaa
     3301 ctcaggatta aaatgtttta ccgattcgcc aacataaccc aaatctagcg aactaatttg
     3361 cgccatttct ttctctgtca gtgagaaatc ccagatagca aaattttctt cgatacgctg
     3421 ttgatgtgtc gatttcggaa taacgaccac tccccgttga atattccaac gcagaatgac
     3481 ttgagaaatc gatttttgat gggcatctgc aatactctga agcagattat tttcaaaggg
     3541 tttatgtcgt ccaccaccta acggagccca tgcttcaggc tgcacgttat aatacttcat
     3601 ggttgccagc gcctctggtt gggcaaaata gggatgcaac tcgacctggt tgaccatcgg
     3661 tttaactctt accgtttcac aaaagttcgc cagaacatga gggtagaaat tggaaacccc
     3721 aatcgctttt aatttgccct cttcataggc atcttcgagt gcacgccacg cactaaaata
     3781 atcgcgcatg gcctggtgca ataaatagag atcgaagtat tctagccccg atttttttaa
     3841 tgatgcttca atacctgctg ctgcaatatc ctgattcaac atatcctgca cccacaattt
     3901 agacgttata aataactctt ctcgggtaca taaaccttca gatatggctt cacgaacggc
     3961 ttcaccgacg gcatcttcat taccgtatac cgctgcagtg tcgatgagtc gataacccgt
     4021 gcggatagca ttcaacactg actgtttaca cacgtcttta tcggtaacct gaaaaacacc
     4081 aaatcccatc atcggcattt tcagattgtt acttaatacg gaaaattcca ctgtcagtcc
     4141 ctccattagg tatgaacaat gagtctacgt taaaacgtaa cctcaaatta gtatgtggat
     4201 tttgatatca cttatgcaaa aaattcatta ataatgtagg actgaaacct ctctattttt
     4261 ggggcaatga agcagacgct atcagtgctt ttgccttggg acttgctgtt tttgatacac
     4321 ttagggccca ggacataacg aaaatgtgcg atatgacaaa gaattaacgg agtatgtgat
     4381 gatcaggcag aagattctac agcagctcct ggagtggatt gagtgcaatc ttgagcaccc
     4441 tatttcaatc gaagatatcg cacagaaatc tggctacagc agacgcaaca tccagcttct
     4501 gttccgaaat ttcatgcatg tgcctttggg agaatacatt cgcaaacgaa ggctttgtcg
     4561 tgccgccatt cttgtccggc tctccgcgaa atctatgctt gatattgcac tctctttgca
     4621 ttttgattca cagcaatcat tcagccgaga atttaaaaag ttattcggct gctctccccg
     4681 tgaataccgc caccgtgatt attgggatct cgcaaatatc ttcccttctt ttttaatacg
     4741 tcaacagcaa aagacggagt gtagattagt caactttcct gagacaccta tttttggcaa
     4801 ttcatttaaa tattatattg aagtgtcgaa taaatcaccg gatgaagaag tcaaactgcg
     4861 acgtcatcat ttagtcagat gtatgaagaa ttttaagacg gatatctatt tcgtctccac
     4921 gttcgaaccg tcaacaaaat cggtcgattt gctcacagtt gaaacttttg ccggtacggt
     4981 atgcaaagaa accgattcag agatgtcgaa agagtggaca attaaccgag gactgtatgc
     5041 ctctttccgc tatgaaggtg agtgggagct ctaccctgaa tggacgcgaa acctctatct
     5101 gatggaatta cccgccagga ggttagccag aatgaacggc agtgatattg agcgatttta
     5161 ttacaatgaa gatttcgtag aaaaggatag caatgatgtt gtttgcgaaa tatttatccc
     5221 tgttcgtccg gtttagttat cgctatatct aaatgaattt ataacgagac tactgtcagg
     5281 caattcaata aataatggcg ttgaagatgt caacgccatt attgtttatt agaatgttac
     5341 tttcatactc ttaatattat ttcatcgcct cctcgcccgt tgccgtattg gcatccaccg
     5401 cggtaatggt cacagtcact ggtttcattt cataccctcc atatgtcaaa gagttgaatg
     5461 tgaatgtgta tgtacctggc ttgttggtac taaatgtacg cgtgattttt ccgctcacga
     5521 aatggtcatt tttcgacggc aggaattgat aatccttctc cgtcactcct tccggagcct
     5581 caatgtcgac ccacacattc cccccaacag ggttatttgc cgagaacagg gtaaccgtca
     5641 ttgttgcggt ctgtacccca tccgccacca gagaagtcac atcagatgat gcgggcgagc
     5701 accgcgtttt tatcatccgc gacgaattta gattcagcag ttttcgtcga ttcctgaccg
     5761 ttggcctgtt ccactttcgc cgtgagtgta tatttcgccg cgacagtggt cgtggcggtg
     5821 aaaatagcct gcccttgctc attagttttc gccgtcccat cgggatccag agctaaattt
     5881 gccgggctgg cagtcagcgt cacctcgcta tctttcaaca ggttgttatt ggcatcagtc
     5941 accgtaactt tatacgtctg tttggctttg ccgtccgcca gagcatagtt gccaatcacc
     6001 tcaataccgg agacggttgc ggtagtctta tcggctacaa acgttatagt ctgcgcatcc
     6061 gaaggctggt catttcctgc cgatgccgtg atctcatagg ttccggcagt cacggaaacc
     6121 acctgcaact ctgctttccc ctcatcgttg gctttcaccc agacattatc gcctgtaagc
     6181 ggcttgacac cccggggcag attaaaggtc accaggctcc ctggaacagg gttaccattt
     6241 ttatcctcaa cgttcgccgt cagcgtaaag gcatctttgc cgtttgccac tttttgagca
     6301 gcggcgtcta cctgcaggtt tgcctgaccg gtgctggcat ccgcgttgaa tttcaccgtg
     6361 accgtcttct gagagttttt caccgcggcc gcgatttcaa gttcccctgc aacggttgat
     6421 atcaactcaa tatccgcttt acctgccgca ttggttgtta ctgtattccc cgtcttgctg
     6481 gtcacaccct gcggtaaagt cagcgtaact tcttgcccct gcaacgggtt accataggtg
     6541 tccacaacgg tcagggttac ctggttagtc gactgtccat ttgccagctg gttatcaacc
     6601 ttcactgtca tatcacgaat ctcagcctta gatgcgtcac cagcaacatt cagcaccagt
     6661 ggttgagcaa cggcattttg gccgttcacg cgcggcatga ctgatagctg gcctgcagcc
     6721 gatcccagcg ttaaggtcgc cacgtagacc ccattacttt gctctgtcca gcttcctgct
     6781 gaaggtcgct ctgatcccgt gctggcggca ccgctaaaca ctggtgcatc gagcttcagg
     6841 ccactaacag ggttcccata cgcatccttc gcggtgaagg taagttccgt cgtcgctttg
     6901 acggtcggag ccttattgtc cgcaacaagc gtagagttcg ctgatgacat ctctccggca
     6961 ataacagtca gctgcgcggc ttcggtggct gctggctggc cgttgaatag cggcatgacg
     7021 agaagctcac ccgtctttcc acctgttgtc agcgtagcga cataggaacc atcacctttt
     7081 tcggtccaac tggaaacagt cgccccttca gacgcagcac ccgtcaggct tgccgacagg
     7141 gaaagaccac tgatagcgtt gccatgtgca tcttttgcaa taagcgttac ggttgtgctt
     7201 tcaccggctt ttacgtgatc ttctgcgaca gagactttcg actggtttga agataatgca
     7261 tcagccacca cggttacttt tgccgcattt gctgccgcat cctgtccatt tagcttcggc
     7321 ataacgtcta attcacccgc cgtagagccg agagtaatct gcgcagtcca cgttccatca
     7381 ccattatttg tccagccaga tgccgtagaa cctacagccg cagcacccgc taatgacggc
     7441 gcttccggcg tgaggctggt cacagggttg tcataggcat cttttgccgt ccagattgcc
     7501 gtaactgtac cgccaaccac cggtttatca ggattcaggg tgatggacga atgtgctgca
     7561 tcaagcggcc cggcaacaaa cttcagcgtt tgttgtaatg tgcccagctg atactgctcg
     7621 ctgaaggcac gcacaacaac gtttccggca cgggtcgaag aaaccgtggc gctgtaaacc
     7681 cctggtttta tttccgaaat ggcaccaacg gttacaccat tagtgtcttg cggaacaagt
     7741 cgcaggcggc tggcttctcc cgtcaccggg ttaccatcag tatccaccgc agtaagcgtc
     7801 agcgtgtggg attgctgacc atcagcaacc acatcacctg acggctcgtt agcgctcagg
     7861 gtggagtttg ccacatccat catcgtggcc cgcagttctg cagtgacagt tttgctcatg
     7921 tcatcaacgc taacagtaat cgttgcctca cctgactgcg ttccggtagt aaagacagac
     7981 tgatacaccc ctgcttcagt ttcggtgaac tcacccagtg ttggctgtgc ctgtgattta
     8041 gtggccttca gggtacgagt cacaatattt ccagctggct tgaacgtcag ctcagtcttg
     8101 atctgatctt tcatgcccgt gactggctgc ccctcggcgt ctcgcagaga aagcaccagt
     8161 ggcttttgct cactaccgtt agcaagcatt tgaatacggc tctgaccgtc aagcgttaac
     8221 gccgtgcgct cggcactcat acctgctccg gtaatgacca cctctgtctg cacgcgtttt
     8281 gaggcattgc ctttgttatc gtaggcaacc gcagaaatcg cataataatt gtctttgcct
     8341 gcctggtaag ccgggagcgt cacttgccac tgattaccct gccccgtaat ttttccgcct
     8401 gcggccagca aagacggcgc ttcccattga acatttttca gaccgtgagt tgctttactc
     8461 accacaagtc ccagggaaac cgtctgcccg cccttacctg caatacggtc aggcagagca
     8521 atacggatca cttcagattt gcgatactca agaacgatgt tgttattacg ctcaaccagg
     8581 tcatagcggc tgcctgccag cattcgacgc tcgcgaatgc tatccgtatc gagttgtttc
     8641 gccaaaggtt cgccaattcg gtagttaact tccaggccaa agcgagtgtc attctcaccg
     8701 ctcttgccct gcttatgccc ggcgctcagt gtcagaagag gcactggtgt ataggttacc
     8761 tcggcagaaa tagcatgcgg gtctttctgg cgtttatctt taccaaacag cccgacttca
     8821 tcgccataat actgttcata catcaggctt gcgccaagct gcggccaggc aggtaaatag
     8881 ccctctgcgc ggatatccca accattcgcc gggcgttcct gataatcctc aatatccggc
     8941 gattttttcc agccagaagc ccgaatataa ccattggcgc tcagtttcaa ataatcgcgc
     9001 cagtattccg caccaacacc aatgcgggta tgactacggg ataaatcatg atcgataaaa
     9061 gtattcaccc ccgccatcca gtcatttcct gaaaaatgac gccagccaaa accaatattt
     9121 gactgagtac gatcgtctgt acgatgtatt gccccctgag tgaacaacat atttgtcggc
     9181 gtatcataaa tcggataaag catttccagc gaagaatcct tcagcgagaa atctttatcg
     9241 acattcagtt tgacgcgcgc tgtaccatat ttcccgagcc actcctgtat ttcctggtta
     9301 gctttagcgg tggccattcc ggtaataaaa ttacgtgtcg catcgctatc tggctgactg
     9361 cttaaaaatg tcccggcatt tgcggcaaac gacgcgacat ttttctccac gttactatca
     9421 gcagttaccg tagtatttcc catgctcaac cgtggctgaa ccgcatgctg cgcacgtgcc
     9481 gccattactg gggtaaaggt gacagcgagt ggaaaaagaa cctgaacaga gatatttgcc
     9541 cacgccacgc agcgggccag aactgaataa cgaaattgtg gttgtttatg acctgtttta
     9601 taacgtgaca ttgagataat ttcctgatga acgaattaac gaacataggc agacacaaat
     9661 aatggcgtct gcttgtcaga aaaaccgggg atttcccggt ttccttttcc gcagaaaagg
     9721 aaataccttt atgtaagaaa gtacaccgcc acctaatata tccagtgcct ggagccactc
     9781 atcaggtggt tgggcgcggg attatatttt tatttatgag taaaaattta gctttttgtg
     9841 caagtcatta aatagatacc taagttatat ttcttactct gcggacagtg aataattcat
     9901 tattcagagt gaataataat gacctgcctt taattagcaa ctattggaaa tacgtcgaag
     9961 taggtatata cgccaggata aaccaatata ttttttatgg atattcattt tccttcatcg
    10021 gactgtaaat tgcgggaata tcgaacttgc acgactaatc tcaaccctca aacagattta
    10081 tgaattttgt tcataacccc tgacaaatcc tgccgtctaa tcgagctctt ccctttacgt
    10141 tatgcttctc atgaacgata acggaaggat aacaccatgc aaaaagtaaa actgaacaac
    10201 ggtattgaaa tgcccctgtt gggctttggt gtatttcaga tgaccgatgc cgccgaatgc
    10261 gaaagagcgg tcattgacgc tattaacagc ggttatcgac tgattgacac cgccgcctct
    10321 tatcaaaatg aaatccaggt cgggaacgca ctgaaacaaa gcggtatcgc ccgtaacgaa
    10381 ctctttgtca ccacgaaact ctggttgcag gataccagtt acgaaggggc caaagcccag
    10441 ttcgaacgct ctctgaatcg actgcaactt gattacgttg acctgtactt gattcaccag
    10501 ccatatggcg atgtccacgg agcctggcgt gccatggaag agctgcaaca ggccggtaaa
    10561 attcgcgcta ttggcgtcag taacttccat cctgaccgac tggcagacct tatcgctttt
    10621 aataacgtcg ttccggcggt aaaccaggtt gaagttaacc ctttcaatca gcagctacag
    10681 gccgttccct ggatgcaaag tcgtggcatt cagccagaag cctgggcacc gtttgcggaa
    10741 gggaaaaatg gtctgttcca gcatcctgtt ctcacggcaa ttggcgagaa gtatggcaaa
    10801 agcgtgggcc aggttgttct gcgctggatc taccaacgag gcattgtttc actggcaaaa
    10861 tcagtgcgaa aagaacgcat ggaagagaac atcaacattc tcgattttga actcagccct
    10921 gaagatatgc tgcaaattac cgccctggat accgcaacca gcgcattctt ctctcaccgc
    10981 gatcccgcaa tggtggaatg gctgactggc cgcaaacttg atgtttaagc cgcgataaaa
    11041 gaggtattca ttcaatgcaa aaacgttatc tgggtaaatc cggactcgaa gtctccgccc
    11101 ttgggctcgg ttgcatgggc ttaagccacg gctacggccc ggcgaccgat acccgtcagg
    11161 ctatcgaact tattcgtgct gcggttgaac gtggcgtcac cttcttcgat accgccgaag
    11221 tgtatggtcc gtatctgaat gaagaagtgg tcggcgaagc cttaaaaccg tttcgtgacc
    11281 gcgtggtaat tgccactaaa ttcggtttta ccttcggcga tgataataag cagcagattt
    11341 taaacagccg tccggaacat atccgtgaag ccgttgaagg atcattacgt cgtctgaaaa
    11401 cagatgttat tgacctgctg tatcaacacc gtgttgatcc agatgttcct attgaagatg
    11461 ttgcaggaac ggtgaaagac ctcatcgcag aaggtaaggt caaacatttc ggcctgtctg
    11521 aagcaggagc acaaaccatt cgtcgtgcac atgcggtaca acctgtcacc gccctgcaaa
    11581 gcgaatattc catgtggtgg cgtgagcctg aacaggaaat tctgccgttg ctggaggaac
    11641 tgggaattgg ttttgtcccc ttcagcccac taggtaaagg tttcctgaca ggggcgatta
    11701 agccgggaac cactttcggc aaagatgatt accgcagcac cgtaccgcgt ttcgcagcac
    11761 aagccattga agccaatgaa aaactggtca ccttgttagg tgaactggct gcggagaaag
    11821 gggtaacgtc tgcacaaatc gcactggcat ggctattagc acaaaagccg tggattgttc
    11881 ccatccctgg caccactaaa ctgaaccggc tggaagaaaa cctggccgct gctgacattg
    11941 ttctttcgca gaaagatacg cagcagataa ccgaggcgct ggaaacaatt aaaatcgtcg
    12001 gtgaacgtta ctcgcctgaa catcaggcac gcgtaggccg ctaataccca aacgggtcca
    12061 ggataaagga cccgttattc ggtgtagcga agcgcatcga tcaacagcgc aaaagctggc
    12121 ggatgctgct tgcggctggg gtagtaaaga taatacccag gaaaagttgg acaccactcc
    12181 tgtaacacct gaataagctt gcctgacttt atatactcct gaaccatgtc ttcagggaca
    12241 caagcgatcc caaaccctga taacgcggca tcaattcttt ctggcaatgg actacacgtc
    12301 agttgcccat caaccttaac gcgaagcggt ttcccttctc gctcgaactc ccagtggtaa
    12361 agcccaccgg cagttggcaa acgcatgttg atacagcgat gattttgtag ctcatgaggc
    12421 gtttcaggcg cagggtttgc agcaaaatac gccggagctc ccaccacagc catccgcata
    12481 tcaggtccaa tcctgactgc aaccatatct ttatcaacat tttcacccag tcgtatcccg
    12541 gcatcaaaac ggccttcaac aatatcgaca aaaccgttat ccaccaccag ttcgacattg
    12601 atttcgggat actctctgag aaaaggtttt agcttcggcc agaccagact tcgcgcggca
    12661 tgttccccgg cagataaccg aatattcccg gagggcgtgc tgttcaattg aataagtgat
    12721 tccagttcct gttcaagctc gtcaatacgc ggttcaaggc aggcaatgat tctctccccc
    12781 gcttctgtgg gagccacact gcgggtagtc cgtgtcagga ggcggatatt gagcctttcc
    12841 tccagagcct tcattgcatg gctaagtgca gactgagaga cgccgagttt gcccgctgct
    12901 ttggtaaaac ttcgctctct tgccaccaca agaaagattt gcagttcgtt gaagttttct
    12961 ttgagcattg gcgatttcct tgtagaagca tcctcttcac agacactcat gaaggggggc
    13021 atagatgcta ttattttaac cacattatta gtaacaatga taatctggct gtatcgaaaa
    13081 taattctaaa ctttcaggag atttattatg aataaacgta tcctcacgat tgcgccattc
    13141 ttcttaccga tattctgaca cacatcatta actttctatt tttactcttt tctccaccat
    13201 aaaatcaata aaagcacgaa ctttagaggg cattctcacc cgtgatgaaa catatatata
    13261 aaatccagga aatgaaacgg cccaatcctc aagaatcggc tgtaacttgc ccgcattgat
    13321 atgttcagcg attgcaaaat ccagatgttg aatgataccc gttccattca gcgccattcg
    13381 tatcatgctt tcgtcatcat tagaaatata cttacctgac acctctattt cgaactcttc
    13441 attacccccc acaacattcg aaaactccca tgctgagagt gcaccgctac ttttatgccg
    13501 caaccgaaga caattatggt tactcaattc ctgaggagtt tcaggaatac cgtgttcttt
    13561 taaataatct ggcgatgcca cagtaaccag tttaataagc ggagaaacag gaacggcagt
    13621 catatgctca tccagtcctt gctcaagacg tatgcccaca tcacagccgt ccgcaataat
    13681 gttagataaa ccgtcatcca taatgagttc tatctttgtt ttggggtaac gagtaagaaa
    13741 ctcgccaata tgaggctcaa caagcatcct tgctgcaaca cgggaggaat ttattctcaa
    13801 cagtcctgta ggttcaagtt gggtatcacc gacatctttc aacgcatcat caatttgccc
    13861 caaagccgac accagcactt catacagacg ctgtccttcc tcagtaagcg acatattgcg
    13921 ggtcgtacgg tatatcagct tggtgttaag acgttcctca agagatttta aattctggga
    13981 caatgcggcc cgcgtaatac ccatttcaga agcggctctg gaaaaactgc cggcacgaac
    14041 aatcagcgca aaccaggcaa gagaaggcaa aattgaaggc tctattttca tcattgtaaa
    14101 ccttaactta acactgtgat agatattaga ccattgatga taaccaatac aagtcatact
    14161 attacgccgt tctcgatatg aacacaaatg ccagttgcgg ataatagaaa tgatatttaa
    14221 atctgtttag gcaaatccct ggagcttgaa atgaaaactg tttcgataaa acacacttac
    14281 tgggatatag ctgctgatat ctatttccct gacgatttta atagtgaaaa aaagtatcct
    14341 gccattatca gtgcccatcc aattggaagc tgcaaagaac agacatcggg tagtgtgtat
    14401 ggtgccgccc tggcaaaagc tggctttatc gtcattgctt ttgatgccag ttttcaggga
    14461 agcagcggtg gagaaccgcg ttatctggaa gatccaacaa tgcgagtaaa agacttcagc
    14521 attgtcgttg attaccttac taccttacct tacgttgatg ccgggcgtat tggtgtactg
    14581 ggtatttgtg gtggaggtgg atatgccatt aatgccgcaa tgacagaacg tcggattaaa
    14641 gccattggta ctgtcacggg tgcaaactac gggcgtctta tgcgtgaggg atttacagcg
    14701 tttaatccta tcggagcact cgaagcaatg gcgcaacaaa gaacagacga agctaacggc
    14761 gcaaaacttc gcgtagatga tttgctccct tcttctccgg aagcagctct tgaagcggga
    14821 ttaacagaaa tagacctttt tgaagcaaca gaatattatc gttcgccacg aggctgtgcg
    14881 cctaatgggg taaatcgttc gctattttca catcagactg ttgccgtggg ctgggatgct
    14941 tttcatcttg cagaagtcct gttaactcaa ccactaatgg ttgtagtggg cgatagagtg
    15001 ggcgcttttg gtgcctatcg tgacggctgc gaaattattg gccgcgctgc ctcaaaacat
    15061 aaagagctgg tcgtggttga aggttattca cattacgacc tgtatgataa gccagaacct
    15121 gtgaaacagg cactcgacaa attaattcct ttttataaaa cacatttgta ataatcttac
    15181 agggagattt cttatctccc tgacatttat ccttcattga ctacacaaca ataatgaaat
    15241 tactcaaaaa gaaatttcat taaagaaaca aaaaagcagt aactcaatca ttaagattat
    15301 tagaaaccct ttgaagggat tataaataaa gccagaaatc ttggttccaa agctggcaac
    15361 aactaaccag ttaaaacatt cgcattaaaa tcagataaac atttgaggta ttgttatgac
    15421 aaataaacac tcatcattgt tttctccttt tatgctgaca gaaaaaataa agctacgtaa
    15481 tcgtatcgtt atggctccta tgacaacctg gtcagctaat cctgatggaa ccatctctga
    15541 gcaagaactc gaattttata aacgtcgctc acaaaatgtt ggtttagtaa taacaggatg
    15601 cacctatgtc acgccaagcg gcattgggtt tactcatgaa tttgccgcgt atgacgaccg
    15661 tttcattaac agccttgaaa agttagcagc tgcagccaaa agtggcggtg ctccggctat
    15721 tttgcagatt ttccacgccg gtaataaagc catcccggag cttgtgccaa ataacgatgt
    15781 tattagtgcc agcgcatcaa gtgtaaaatc tggtgacttt atgaagagag tggtacaaag
    15841 cagggaaatg actgaaaacg aaattcagga aaccattaga gcattcggag atgtcacaaa
    15901 aagggcgatt aaagcaggtt ttgatggcgt tgaacttcat ggtgcgcacg gattccttct
    15961 gcagaatttc ttctcccctc tatttaacca gcgtaatgac cgatggggag gagatcttga
    16021 aggacgtatg cgcttcccgc tggccgtatt acaggaagta aaaaatgtag tctatgaata
    16081 tgcgacaaag ccctttgcta tcggttacag aatatctcct gaagaatctg taacaggggg
    16141 gctgcggata gaggatactt ataaactcct tgatcggctc atttcatccg gtatttcgta
    16201 tatacatacc tcgctggtca gtattaacga tagctaccct gttgagtcgc caaatggccc
    16261 ccgaacaatt gaattgattt taaatcatat tgcaggcaga gtgccggtaa tcgccgcagg
    16321 taaaatacgc acaccgtctc aggctcaaga agcaatttcc gctggcctgc cacttgttgc
    16381 gatcggtaaa gggctggtga taaatccaga atgggtgact ttagctgaat caggtagaag
    16441 tcatgaaatt caaacagccc ttaatccaca acgcgttcct gaattaacca ttccggataa
    16501 actatgggat caaattcagg catcgaaagg aactggctgg tttccgttaa tagattaaat
    16561 atcgattact ctcccgagag taaaactatc aaatcaatga gtaaccgata cagcctcaca
    16621 aattctttca gataattaac ccgtcactgc ggcacgttct ctcgtaaggc gtgcctgcag
    16681 tgtaatccag tatgtgtccg gcatagttga cggtttaata gaactcaatt cctctcccag
    16741 gtcaccaaca acgatatctt gtataacacc ccaggagata cgacattatg atgagatgcg
    16801 atatgctcct cagcgccaat caacgccagg gctgggccag tcttgagatc agactcattc
    16861 ccgaaactga cttcaggcga catatatcaa tgacgttggt gcaataaaag atagccgatg
    16921 taatacatct attcaaattt gtgatacatg tcaaaaaatt ggttgaccaa actcgttatt
    16981 tatataaggg cacttacgaa gtgcactctt ttttaaagcg aggaagtacc aatgaaagag
    17041 aataaagtac agcaaatcag tcataaactg attaatatcg ttgtttttgt cgcaattgta
    17101 gaatacgcct atttatttct ccatttctat taataacggc aataaactgt tcacttcagt
    17161 gatatttaaa atatgcatcc tctccctttt ttgtaagtaa ttattatatc cgtgggagag
    17221 gaatatacgc tgtcaggtaa tcaatcaaac tgcgataaat catcggccag taaagtggag
    17281 atagcctcca ttctcgaaaa atccatactc tcagcgaaac catcatcaat cactcatcca
    17341 ggcgtttatg ggagcgtagc caatggctgc taacaatgcc agacttcccc gttgccgaaa
    17401 ttccacatcc cacaaatagt cacagggatt gggtgttgaa atgatccgga tgagcatgta
    17461 tctttatggt tatgttataa cataacaggt aaaaatgatg aagcccaata tccatcctga
    17521 gtatcgtact gtggtgttcc acgacaccag tgttgatgag tactttaaaa tcggctcgac
    17581 tatcaaaaca gaccgtgaga ttgagctgga tggcgtaacg tatccgtacg taacaattga
    17641 tgtctcttca aaatcgcacc cgttctatac agggaagctg agaacagtgg catcagaagg
    17701 aaatgttgca cgattcaccc aacgttttgg tcgttttgtt agcacgaaaa agggggcgtg
    17761 atgaaagtcc ttaactctct gcgtaccgca aaagaacgcc atccagactg tcagattgtg
    17821 aagcgaaaag gacggctgta tgtgatttgt aaatctaatc cacgttttaa ggccgttcag
    17881 ggtcgtaaga aaaaacgttg atttaaaatt cgacgaatta acgatatttg tctgattaat
    17941 aatcagaccg gattaatgtt ggtgtattta ttacaccagc attaattttc ctggggatgt
    18001 atcttcctgt tcatttaagg tcaactgcct gacgtttctc tccgaatatt ccattatatt
    18061 aatgttgact tgttgaccag cttcgccctg tatgctggca tcaactctat tttagactga
    18121 acacgtcact cagtttcctc cctttgcgac gcagcctgca tttacactca aactattaag
    18181 atgataaatg tggtaaatct gttggtactg gcataaaaac gtttgcgcca caggaacgga
    18241 ctgatccatc ggtaacccca tcaccgacgt tcgagtgcca gttagagtaa cgcgcacaga
    18301 tgactgaatg cagtgccctg gcaaagaggc catcgttcct gtgacaactg gcagtcttcg
    18361 tttaacttca cttaatttgg ctcttagggg gcttaccgac atgatgacgt acttacacct
    18421 gtttaatttt tcatcactta ttggaatgaa cacccataac cattttgtgc ggcatgttaa
    18481 cccattaaaa cgccttactg attggcaaat catctttaat tatgactcat gatagtttta
    18541 tattctattt cttatcattt aaacttgttt ttttactagt ccattacaca acacattaag
    18601 actattccta acacctcagg gcaaagttcc tggctaatat aaaatgcaag taagaattga
    18661 acgttatatt gccaataacc ttatgaaact gaatgtcttt ttcttcttat caaaaaagca
    18721 atattttcat tttttgtaaa tattgactta accatggaat tcattttctg ttcacatatt
    18781 gacactcatc aggaaaaaaa cataaattta aacctaatcg aaataattaa aacttaatct
    18841 cgtttaacct atattgatat gtgctacgta tcttatttac ttccgattta ctaaagaaac
    18901 tgaatgtacc tgtaaaaatt acaggtttgg aaagtagtga catggcaaaa tgattacagc
    18961 agggactatg aggttaaaaa ccatatggaa tgtcaaaacc gttctgataa atacatctgg
    19021 tctccccatg acgcctactt ctataaagga ctatctgaac tgattgtgga tatcgacaga
    19081 ttaatttatc tatcgctgga gaaaatcaga aaagatttcg tgtttatcaa tctcaatacg
    19141 gattctttaa ctgagtttat aaaccgtgat aatgagtggt tatccgcggt aaaggggaaa
    19201 caggtcgtat tgattgcggc cagaaagtca gaagccttag caaattattg gtattacaac
    19261 agcaatatta ggggcgtggt atacgctgga ctgagtcgtg atattagaaa agaactggcc
    19321 tatgtgatta atggcaggtt cctgagaaaa gatattaaga aagataaaat cactgaccgg
    19381 gaaatggaaa ttatccgcat gacggctcag ggaatgctgc ctaaatcgat tgccagaatt
    19441 gaaaattgta gtgtaaagac agtgtatacc catcggcgta atgcagaggc caagctgtac
    19501 tcaaaattat ataagttggt tcagtaaact ccaggcaagt tagttttaaa aaatgactca
    19561 ccgggacttc atgtcctcaa ttcaactcgg gaagaaaagc aatgaaaaaa aaggttctgg
    19621 caatagctct ggtaacggtg tttaccggta caggtgtggc gcaggctgct gacgtaacag
    19681 ctcaggctgt agcgacctgg tcagcaacag ccaaaaaaga caccaccagt aagctggttg
    19741 tgacgccact cggtagcctg gcgttccagt atgccgaagg cattaaaggt tttaactcac
    19801 agaaaggtct atttgacgtg gctatcgagg gtgactcaac ggctaccgcc tttaaactga
    19861 cctcacgtct tatcaccaac accttaaccc agttggatac ctcaggttcc acactgaatg
    19921 tgggcgtgga ttataacggc gcggcagtcg aaaaaactgg cgataccgtg atgatcgata
    19981 ccgccaacgg cgtactgggc ggcaacctta gcccgctggc taacggttac aatgtcagca
    20041 atcgtaccac cgcacaggat ggtttcactt tctccatcat cagcggcacc accaatggta
    20101 ccaccgcagt aaccgattac agcactctac cggaaggcat ctggagcggc gacgttagcg
    20161 tacagttcga cgcgacctgg accagttaat ctctctgatg taccagcagg ggtagccccc
    20221 ctgctttatt cctggacgga attgttatga aaaagcacct tctgcctctc gctttgctgt
    20281 tatccggaat atctccggcc caggcgctgg atgtcggcga tatatcatcg tttatgaaca
    20341 gtgacagcag cacgctgagc aaaacgatca aaaacagtac cgacagtggt cgcctcatca
    20401 atatccgtct cgaacggctc tcttcaccgc ttgacgacgg gcaggttatc tcaatggaca
    20461 agccggatga gttgctactc actcccgcta gcttgctgct acccgcccaa gccagcgaag
    20521 tgatccgctt cttctataag ggacccgcag atgaaaaaga gcgctactac cgcattgtct
    20581 ggtttgatca ggccctcagt gatgcgcagc gcgataatgc caaccgcagc gctgtggcca
    20641 ctgcttccgc ccgcatcggc accattctgg tcgtcgcacc ccgccaggca aactaccact
    20701 ttcagtacgc caacggctcc ctgacaaata caggaaatgc gacgctgcgg atcctcgcct
    20761 acggcccctg cctgaaagcc gccaatggta aagagtgtaa agagaattac tacctgatgc
    20821 cgggcaagtc gcgtcgtttt acccgcgtgg acacggcgga taacaaagga cgggttgcac
    20881 tttggcaggg tgataagttc attcccgtga aatagatagc tgtgcagatg gataacgaca
    20941 atgcctttac gacggttctc cccaggactg aaagcccagt ttgccttcgg catggtcttt
    21001 ttgttcgttc agcccgatgc cagcgctgct gacataagtg cgcagcaaat aggtggggtg
    21061 attattccgc aggccttcag tcaggcgctc caggacggca tgagcgtccc gctttatatt
    21121 catctcgccg gtagccaggg tcgccaggac gatcagcgaa tcggcagcgc ttttatctgg
    21181 ttggatgatg gacagctacg catccggaaa atacagctgg aagagagtga agataacgcc
    21241 agtgtcagcg aacaaactcg acagcagctg atgactctgg ccaacgcccc gttcaatgag
    21301 gcccttacca tccccctgac tgacaacgcg cagttggatc tcagcttgcg ccaactgctg
    21361 ctgcagctgg tggtcaagcg cgaagcgctg ggcaccgtac tacgctcacg tagcgaagac
    21421 atcgggcagt ccagtgttaa caccctcagc agtaatctga gctataactt cggcgtctat
    21481 aacaaccagt tgcgtaacgg cgggagcaac acatccagct atctgtcgct gaataacgtt
    21541 actgcactgc gcgaacatca tgtggtgctc gacggctcgc tgtacgggat cggtagcggt
    21601 caacaggaca gtgaattata taaagcgatg tatgaacgcg attttgccgg tcaccgattt
    21661 gccggtggaa tgctcgacac ctggaacttg cagtccttag ggccgatgac cgccatttca
    21721 gcagggaaga tttacggcct ttcctgggga aaccaggcca gctccaccat cttcgacagc
    21781 agccagtcag ccacgccagt gatcgccttt ttaccggcgg cgggcgaagt acatctcacc
    21841 cgtgatgggc ggttactaag cgttcagaac ttcaccatgg gcaatcatga agtggatacc
    21901 cggggtctac cgtacggtat ttacgatgtg gaagttgagg tcatcgttaa cggtcgcgtg
    21961 attagcaaac gcactcaacg ggtcaataag ctgtttagcc gggggcgcgg cgtcggtgca
    22021 ccactggcgt ggcagatatg gggcggtagc tttcatatgg atcgctggtc ggaaaacggg
    22081 aaaaagacgc gaccagctaa agagagttgg ctggcaggtg cctcgacctc cggctcactg
    22141 agtacgctta gctgggcggc aacgggatat ggatacgata atcaggcggt gggtgaaacc
    22201 cgtctgacgc tgccgcttgg gggggcgatc aacgttaacc tgcaaaacat gctggccagt
    22261 gacagctcat ggagcaacat cgccagcatc agcgccactc tacctggagg cttcagttcg
    22321 ctgtgggtta accaggaaaa aacccgcatt ggcaatcaat tgcgacgtag cgatgccgac
    22381 aaccgtgcaa tcggcggcac actcaacctg aactcactgt ggtcgaagct gggtacgttc
    22441 agcatcagct acaatgatga ccgccgctac aacagccatt attacacggc agattactat
    22501 caaaatgtct acagcggtac ctttggttcg cttggcctgc gggccggtat tcagcgctat
    22561 aacaacggcg acagcagcgc caatacaggg aaatatatcg ctctcgatct ctcgctacca
    22621 ctgggcaact ggtttagcgc agggatgacc catcaaaacg gctacaccat ggcaaacctg
    22681 tcagcacgca aacagtttga tgaaggaacc attcgcactg ttggtgccaa tctgtcacga
    22741 gccatctccg gcgataccgg tgatgacaaa actctcagtg gtggggcgta tgcacagttc
    22801 gacgctcgtt acgccagcgg aacgctgaac gtcaatagcg cggcggacgg ctacatcaat
    22861 actaatttga ccgccaacgg cagcgtcggc tggcagggta aaaacattgc tgccagcggg
    22921 cggactgatg gcaacgctgg ggtgatattc aacaccgggc tggaggacga cggtcagatc
    22981 agcgccaaaa tcaacggacg gattttcccg cttaacggca agcgtaacta tctcccgctc
    23041 tctccctatg gaagatatga ggtggagtta cagaacagca aaaactcact cgacagttac
    23101 gatatcgtca gcggtcgcaa aagtcatctg actctctatc caggcaatgt cgctgtcatt
    23161 gagccagagg tgaagcagat ggttaccgtc tccggtcgta tccgtgcgga agacggcaca
    23221 ctgctggcta acgcacggat taacaaccat atcggccgaa cccgaaccga tgaaaacggc
    23281 gagtttgtca tggacgtgga taagaaatac cccactatcg attttcgcta cagtggcaat
    23341 aaaacctgcg aagtggcact ggaactcaat caggcgcgcg gtgcagtctg ggtcggtgat
    23401 gtggtctgca gcggcctctc atcgtgggcg gcggtgacgc agacaggaga agagaatgag
    23461 agttaaccta ctgatagcga tgataatctt tgcgctaatc tggcctgcaa ccgcgctcag
    23521 agcggcagtg agcaaaacaa cctgggcgga tgctccggca cgcgagtttg tgtttgtcga
    23581 aaacaactca gacgacaact ttttcgtcac tcctggcggg gcgctggatc cgcgcctgac
    23641 cggtgccaac cgctggaccg gtttaaaata caatggttca ggaaccatct atcagcaaag
    23701 cctcggctac attgataacg gttacaacac cggcctttat accaactgga agtttgatat
    23761 gtggctggaa aattcaccag tttcatctcc tttaactggc ttgcgctgca tcaactggta
    23821 cgctgggtgt aatatgacca ccagtcttat cctgccgcaa accaccgacg ccagtggatt
    23881 ttatggcgcg acggtgacca gcggcggcgc gaagtggatg cacggcatgt tgtcagacgc
    23941 gttttaccag tatctgcaac aaatgcccgt cggcagcagc tttacaatga tcatcaatgc
    24001 ctgccagacc tctgtgaact atgatgccag cagcggcgca cgctgtaagg atcaggcctc
    24061 cggcaactgg tatgttcgca acgtcaccca tacgaaagca gcaaatctgc ggttgataaa
    24121 tacccactcg ctggcggaag tatttatcaa cagcgacgga gtaccgactc tgggcgaagg
    24181 gaacgccgac tgccggacgc aaaccatcgg cagccgttca ggattaagtt gtaagatggt
    24241 taactatacc ctgcaaacaa acgggctcag caacacctca atccatatct tcccggcgat
    24301 cgccaactcg tcgttagcct cggccgtcgg ggcgtacgat atgcagttca gtctgaatgg
    24361 cagttcatgg aaaccggtga gcaataccgc ctattactac accttcaacg agatgaagag
    24421 cgcagactcg atctatgttt tcttctcgag caacttcttt aagcagatgg tgaacctggg
    24481 gatcagcgat atcaacacca aagatctgtt caactttcgc tttcagaaca ccacatcacc
    24541 ggagtctggc tggtatgaat tttctacctc caacacgctg attatcaaac cccgtgattt
    24601 cagcatcagt attatctccg atgaatatac tcagacaccg tcgcgggagg gatatgttgg
    24661 cagcggcgag tcggcactcg atttcggcta tatcgtaacc accagcggta aaacagctgc
    24721 cgacgaagtg ctgatcaagg tgaccggacc cgcgcaggtg attggcgggc gctcctattg
    24781 tgtcttcagc tccgatgacg gtaaggcgaa agtaccgttc ccggcgacgc tttcctttat
    24841 tacccgcaac ggagctacaa aaacctacga tgccgggtgc gatgatagct ggcgggatat
    24901 gaccgatgcg ctgtggttga ccacaccgtg gactgatatc tctggcgaag tggggcagat
    24961 ggataagacc acagtcaaat tttcgattcc aatggataac gccatttcgc tgcgtacggt
    25021 agatgataac ggctggtttg gcgaagtcag cgcttcagga gagattcatg ttcaggcgac
    25081 gtggcgtaac attaactaag gccctgctga cagtggtctg tatgctggcg gcacctttga
    25141 cacaggcgat ttcggtcggc aatctgacat tttcgctgcc gtccgagact gactttgtca
    25201 gcaaacgtgt agtgaataac aacaaaagcg cgcggatata ccgtattgcc atcagtgcta
    25261 tcgatagccc gggcagcagt gaattgcgca ctcgaccggt ggatggtgaa ctgcttttcg
    25321 ccccccgcca gctggcgttg caggctggtg agagcgagta ttttaaattt tactatcatg
    25381 gtccacagga taaccgcgag cgctactacc gggtctcatt tcgcgaggtc cccactcgca
    25441 accagacaag acgtagccca accggcggcg aggtcagcac agagccggtg gtggtgatgg
    25501 ataccattct ggtagtacga ccacgtcagg ttcagtttaa atggtccttc gaccaggtga
    25561 caggaacggt aagtaacacc ggcaacacgt ggtttaagct actgattaaa ccaggatgtg
    25621 attcgaccga agaggaaggc gatgcctggt atctacgtcc gggagacgtg gttcatcagc
    25681 ctgagttacg tcagccgggg aatcactatc tggtctataa cgacaaattc attaagatta
    25741 gcgattcctg tccggctaag cccccttcgg cggactaaga cttatccatc agacgaggga
    25801 agaattccac ctcagagact caaaaatcgt ttaaatgatg tggaagcgat cgtcaatgtc
    25861 acctgtaacg atgagctttc tcatatgcac accacagtgt tcaggagaca cggaaaggta
    25921 ccaggcggcc tgctaacgag tggtttcttc acgaattagg acgtaaaatc ggtaacggct
    25981 ggaaatcatt caatactcac actatcgaaa gtacaccagc caaccgcgat acgtccctgc
    26041 atacgacgta ccgcagttct ctttacgatt tatagctgta ctggtgaatt atgagcaatc
    26101 tgaatccatg catgacgtgt ggtgcctgtt gtgcattttt ccgcgtctct ttttactggg
    26161 ccgaagccga cgatgctggc ggtaccattc ccgccaggct cactgaacaa atatcccctt
    26221 ttcaccgatg catgagcggt accaatcaga aaaacccccg atgtattgcc cttgcaggaa
    26281 caccgggcaa aaatgcctgc tgcacgatat ataaaaatcg atcttccacg tgcagagaat
    26341 tcgccatgtc tggtgagaac ggagtcgtca atgaggcttg caatcgtgca agagctaaat
    26401 acgggctgac accgttataa atatgcaata attaattgca ctgccccgcc agctataatc
    26461 gcggggcttc gtttttcagg gataacaaaa cccgaaactt cttccatctg ccagcaacaa
    26521 tgcctcttcc tctatagtgt tctccggatc tacagcatcc tgaagtagcg cgatgtactc
    26581 actggctatc tgcttcagaa agtaaaaagc aatgttttta acctataaaa atggcgctgc
    26641 atttgcgcca tttttattat tcaatgcatt atctgtttga gcctaaaggg atctcagggt
    26701 ctggctcatg agtaattctg tttcgtaaat ctctgcgaat aatttcaatg gaccagaacc
    26761 agactaaatg tccaaaaatt tcagagacat tctcatacca cgggagatca aacagaggtg
    26821 gcgtcagtcc catgagaggg aatgaaatca tatgaacaaa aagttgggct aaagcacctg
    26881 ccagtaagcc ctgccaaagt ttaatttttg gaaatacttc agcgaccaca caataaccga
    26941 cagcaaacac tatcgaaaag ataatgtgcg taacaccaac ccagttaaag acatgcccgg
    27001 caaaggtata aacagccgca ttgggatctg tcagccctag ccagtctcga agaaaaatat
    27061 acggaggatt gagaaaatta cgggagcaat caatttgccc agcagccctg attaatgatt
    27121 ccgggccaca cgctgcatta aacatatcca ccgggctacg tggcggcaat ggaacttctg
    27181 ccccccactt aacaaatgcg gaaacaacgc cagcaatcag cccaatgaat gcagcaagac
    27241 cataacgtct gcggtttggt ggagtttgtt caaatatatt catatctacc ctgtttgttt
    27301 aattatgttg tacacctctt caggagtatt cataaaacaa ggcaaatgta aagaactgta
    27361 ttgttttgta taaccacatg gttacctaat cgccaattaa tataagctcc attatttctc
    27421 catattttta tgttagaagt gacaaggaga aatgtaatgt ttctctcaga gaatataaat
    27481 cattcctttt gcccacctta atggtgggct tttttatgca aaacctgaac gtcctgcggt
    27541 attcccctgg cattatgact ttcgcgttta accaacagcc aaaccagcaa acacgccacc
    27601 acaggcacag caaaatccat caggcttgcc acaccccatg cgtgcggctc aaagctgccc
    27661 cgccatggca tattaatccg cttgccattt tcgaacagct gattggttta gtccggtgtt
    27721 ttcctgcttg cacttaaaaa tgagcggatg ggaaactctc gaaatggttc gtcgacatgc
    27781 tcacctttca gtcaggcatt tcaccgcgca cgcgagcaaa atcaaaggca caagaggaga
    27841 acgtagttta cttaaatgtg agataactta ttgatttaaa tggtgccgat aagaggaaca
    27901 taacacaccg catattctct ttgataaata tatatatttt cttgtttcat ctttataaat
    27961 gccccctttt gtgcccccac cttgtttttc atgcgactcg atgagacttt catcgagtcg
    28021 ttaatctcat caaattcttc acgtatcgtg gaaaccttcg cagatctcca tcccctcaca
    28081 gccgtgatcg gctaagaaca atattactta cctccctgta agaacaccgg ctcatcagcc
    28141 cgcacgcttt cctagcttga agaatgtctg tcgtagtcaa atatggtgct tgtacctgag
    28201 aggtaaatcc attgcgatcg atacgaacta actcatattt ctctacaaga aagttaacgg
    28261 cattagccag agtgataccg gcatcgatat gctcctttat aactgtttca tcgctaaaag
    28321 gtgtgtcgct taacattagg ccatagtgct gctcaagtag acgagtaagt aacatttgcc
    28381 agacagacac gggcgacaga cagggcttcg ccgcctgcgg agtcgttgca ggtagagttt
    28441 tcatgtttgc tctcgggaaa ttagttagcg ttgagtggag tagacgacga tgtatacgta
    28501 gccgcaactg ccaagggtat cggcttcgca ggtgaaacca tcgtggtaca gggtgacgca
    28561 gtgggcgtgg cgaggattca tttcaccagt ggtaagcatc gattccatct ggctgataaa
    28621 gtgcgggaat gtttcatcca gcttccggta ttcggcgtca ctgaatttac cggtgatact
    28681 ggcccggtca gccaaataat gcagttggtt gccctcctgc accagacgtg ctcccaggcg
    28741 cggtgtgata tcccgctgca ggccccatgt gatgttactc attgataaac ctcttttatt
    28801 gtcaattcag ggtaatactc atcaggcagg catacggtcc gctgcggtcc tggcggcgtt
    28861 cggcgtatac caccagcacg ccgggaatgt caggaacagc cagaccggtg taatgacaga
    28921 cactgccatg ccactggtat ctgccggtgc agtatcgaaa aatgcgggag gccggatgct
    28981 gacggtaaat cgtcatcgcc cggcgtttac tgataattct catgtcatac ctcaaagcag
    29041 accgtgttca gcgaacgaca atatctgcgt accaccgacg ataaggtggt caggcacacg
    29101 gatatcaacg agctgaagcg cctgcaccag acgctgcgtg atggtcttat ctgccttgct
    29161 gggcgaggtg tctccggagg gatggttatg cgccagtatc actgctgccg cattgaagta
    29221 cagggcgcgt ttgaccacct cacggggatg tacctcggta ctgctaatag aaccggcaaa
    29281 cagggtttcg tgggcaatca actggttctg ctggttcaga tacagcacca taaactcttc
    29341 gcgctccagt cccgccattt tcagtttcag ccagtcgcgg gcagcctgag tggaggtgaa
    29401 tgccacgcct gtttcgcgca ggtgtcggtc aagcagcgtt aaggcgcgtt ttaccgtgcg
    29461 ttgtgcggtt agtggtaatg ccggagccag tgggataagt gactgttcca tcaaaagtat
    29521 ccttaatcaa taatgcgcag aatggcgctg gattctgggt gctgcagaga gtaatcccgc
    29581 agccggtaat agtgttcagt catggcatcg cattcggtgt gacaggcgtg atggctgtat
    29641 tcaatcaggc agacagcaat acctgcggct tctgcgctca tttccgcacc attgccgttc
    29701 atgccgttga acagatgcca tttatcgccc ccctcagcat caggggccat aaacgcaccg
    29761 ccattgctga gcgtgtaaaa ggaccagaga ccaccgctgt actcatcaca gaagcgatcc
    29821 atccaggcga aaatacgggg ttccagcgtt atccactgcg tgatagcacc aaagtgcagc
    29881 ggccagaaat cgagacgctg ctcgtcaggt actggtgttg cagtgaggtc aaattgaggt
    29941 tcattagcgg acatcaattc gcattgcgtt tgtgtcgtca tggatattct ccgtcaataa
    30001 aaacgccagc gacgatggct ggcgtatggg aaataaagga catgcaggga tgatggatgg
    30061 gtgtaatcag gaggaggatg ctctgaggat gccgtactgt cggatataac ggttatgtcc
    30121 ttcacctgca tcaggttcaa agcaccaggc ccgccagacc attcaacctt cagagtctcg
    30181 aaccaccagg cggaagtggc tgcgctggtc atcttcaata gcgatatttt tataccgtct
    30241 ggtaaccgct tcagcctgct gtcgggtgaa cggtcccggt ggcaacatat gagattcaga
    30301 cattcggtgt accttaaaag cacaagcccc tgacgcagac gcgtcagggg ctttgggagt
    30361 atgcaggtgt cgggtgattg tcagtaaccg gtttgtacct tatacagccg tagggtttta
    30421 ctgccccgga caattttcac atacagggca ttatctttcg tgcctcgcac aaaaccggcc
    30481 tgcacatcgc ccttacagtc ac
//
LOCUS       DAMZFT010000052        29658 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.052, whole genome shotgun sequence.
ACCESSION   DAMZFT010000052 DAMZFT010000000
VERSION     DAMZFT010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 29658)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29658)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29658
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.052"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(110..811)
                     /gene="lolD"
                     /locus_tag="PN977_002413"
     CDS             complement(110..811)
                     /gene="lolD"
                     /locus_tag="PN977_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415635.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter
                     ATP-binding protein LolD"
                     /protein_id="HDI6042436.1"
                     /translation="MNKILLQCDNLCKRYQEGSVQTDVLHNVSFSVGEGEMMAIVGSS
                     GSGKSTLLHLLGGLDTPTSGDVIFNGQPMSKLSSAAKAELRNQKLGFIYQFHHLLPDF
                     TALENVAMPLLIGKKKPAEINSRALEMLKAVGLGHRANHRPSELSGGERQRVAIARAL
                     VNNPRLVLADEPTGNLDARNADSIFQLLGELNRLQGTAFLVVTHDLQLAKRMSRQLEM
                     RDGRLTAELSLMGAE"
     gene            complement(804..2003)
                     /gene="lolC"
                     /locus_tag="PN977_002414"
     CDS             complement(804..2003)
                     /gene="lolC"
                     /locus_tag="PN977_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004976588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolC"
                     /protein_id="HDI6042437.1"
                     /translation="MYQPVALFIGLRYMRGRAADRFGRFVSWLSTIGITLGVMALVTV
                     LSVMNGFERELQNNILGLMPQAILSSEHGSLNPQQLPETAVKLDGVNRVAPITTGDVV
                     LQSARSVAVGVMLGIDPAQKDPLTPYLVNVKQTDLEPGKYNVILGEQLASQLGVNRGD
                     QIRVMVPSASQFTPMGRIPSQRLFNVIGTFAANSEVDGYEMLVNIEDASRLMRYPAGN
                     ITGWRLWLDEPLKVDSLSQQKLPEGSKWQDWRDRKGELFQAVRMEKNMMGLLLSLIVA
                     VAAFNIITSLGLMVMEKQGEVAILQTQGLTPRQIMMVFMVQGASAGIIGAILGAALGA
                     LLASQLNNLMPIIGVLLDGAALPVAIEPLQVIVIALVAMAIALLSTLYPSWRAAATQP
                     AEALRYE"
     gene            2264..3337
                     /locus_tag="PN977_002415"
     CDS             2264..3337
                     /locus_tag="PN977_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HDI6042438.1"
                     /translation="MKQKELWINQIKGLCICLVVIYHSVITFYPHLSTFQHPLSEVLS
                     KCWIYFNLYLAPFRMPVFFFISGYLIRRYIDSVPWGNCLDKRIWSIFWVLALWGVVQW
                     LAISALNQWLAPERNLSNASNAAYADSTGEFLHGMITASTSLWYLYALIVYFVICKIF
                     NRLALPLFALFVLLSVAVNFVPTPWWGMNSVIRNLPYYSLGAWFGATIMTCVKEVPLR
                     RHLLMASLLTVLAVGAWLFTISLLLSLVSIVVIMKLFYQYEQRFGMRSSSLLNVIGSN
                     TIAIYTTHRILVEIFSLTLLAQMNAARWSPQVELTLLLVYPFVSLFICTVAGLLVRKL
                     SQRAFSDLLFSPPSLPAAVSYSR"
     gene            3465..6911
                     /gene="mfd"
                     /locus_tag="PN977_002416"
     CDS             3465..6911
                     /gene="mfd"
                     /locus_tag="PN977_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HDI6042439.1"
                     /translation="MPEQYRYTLPVKAGEQRLLGELTGAACATLVAEIAERHAGPVVL
                     IAPDMQNALRLHDEISQFTDQMVMNLADWETLPYDSFSPHQDIISSRLSTLYQLPTMQ
                     RGVLIVPVNTLMQRVCPHSFLHGHALVMKKGQRLSRDALRTQLDSAGYRHVDQVMEHG
                     EYATRGALLDLFPMGSELPYRLDFFDDEIDSLRVFDVDSQRTLEEVEAINLLPAHEFP
                     TDKAAIELFRSQWRDTFEVKRDPEHIYQQVSKGTLPAGIEYWQPLFFSEPLPPLFSYF
                     PANTLLVNTGDLENSAERFQADTLARFENRGVDPMRPLLPPQSLWLRVDELFSELKNW
                     PRVQLKTEHLPTKAANANLGFQKLPDLAVQAQQKAPLDALRKFLESFDGPVVFSVESE
                     GRREALGELLARIKIAPQRIMRLDEAKDRGRYLMIGAAEHGFVDTVRNLALICESDLL
                     GERVARRRQDSRRTINPDTLIRNLAELHIGQPVVHLEHGVGRYAGMTTLEAGGITGEY
                     LMLTYANDAKLYVPVSSLHLISRYAGGAEENAPLHKLGGDAWSRARQKAAEKVRDVAA
                     ELLDIYAQRAAKEGFAFKHDREQYQLFCDSFPFETTPDQAQAINAVLSDMCQPLAMDR
                     LVCGDVGFGKTEVAMRAAFLAVDNHKQVAVLVPTTLLAQQHYDNFRDRFANWPVRIEM
                     ISRFRSAKEQTQILAEVAEGKIDILIGTHKLLQNDVKFKDLGLLIVDEEHRFGVRHKE
                     RIKAMRANVDILTLTATPIPRTLNMAMSGMRDLSIIATPPARRLAVKTFVREYDSMVV
                     REAILREILRGGQVYYLYNDVENIQKAAERLAELVPEARIAIGHGQMRERELERVMND
                     FHHQRFNVLVCTTIIETGIDIPTANTIIIERADHFGLAQLHQLRGRVGRSHHQAYAWL
                     LTPHPKAMTTDAQKRLEAIASLEDLGAGFALATHDLEIRGAGELLGEEQSGSMETIGF
                     SLYMELLENAVDALKAGREPSLEDLTSQQTEVELRIPSLLPDDFIPDVNTRLSFYKRI
                     ASAKTENELEEIKVELIDRFGLLPDPARTLLDIARLRQQAQKLGIRKLEGNEKGGVIE
                     FAEKNHVNPAWLIGLLQKQPQHYRLDGPTRLKFIQDLSERKTRIEWVRQFMRELEENA
                     IA"
     gene            7056..8018
                     /gene="ldtC"
                     /locus_tag="PN977_002417"
     CDS             7056..8018
                     /gene="ldtC"
                     /locus_tag="PN977_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtC"
                     /protein_id="HDI6042440.1"
                     /translation="MMIKTHFSRWLTFFTFAAAVALALPAKANTWPLPPAGSRLVGEN
                     KFHVVENDGGSLEAIAKKYNVGFLALLQANPGVDPYVPRAGSVLTIPLQTLLPDAPRE
                     GIVINIAELRLYYYPPGKNSVTVYPIGIGQLGGDTLTPTMVTTVSDKRANPTWTPTAN
                     IRARYKAQGIELPAVVPAGPDNPMGHHAIRLAAYGGVYLLHGTNADFGIGMRVSSGCI
                     RLRDDDIKTLFSQVTPGTKVNIINTPIKVSAEPNGARLVEVHQPLSEKIDDDPQLLPI
                     TLNSAMQSFKDAAQTDAEVMQHVMDVRSGMPVDVRRHQVSPQTL"
     gene            complement(8100..8357)
                     /gene="bhsA"
                     /locus_tag="PN977_002418"
     CDS             complement(8100..8357)
                     /gene="bhsA"
                     /locus_tag="PN977_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple stress resistance protein BhsA"
                     /protein_id="HDI6042441.1"
                     /translation="MKNVKTLIAAAILSSMSFASFAAVEVQSTPEGQQKVGTISANAG
                     TNLGSLEEQLAQKADEMGAKSFRITSVTGPNTLHGTAVIYK"
     gene            8598..9230
                     /gene="comR"
                     /locus_tag="PN977_002419"
     CDS             8598..9230
                     /gene="comR"
                     /locus_tag="PN977_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family copper-responsive transcriptional
                     repressor ComR"
                     /protein_id="HDI6042442.1"
                     /translation="MATDSTQCVKKSRGRPKVFDRDAALDKAMKLFWQHGYEATSLAD
                     LVEATGAKAPTLYAEFTNKEGLFRAVLDRYIDRFAAKHEAQLFCEEKSVESALADYFA
                     AIANCFTSKDTPAGCFMINNCTTLSPDSGDIGNTLKSRHAMQERTLQQFLCQRQARGE
                     IPTHCDVTHLAEFLNCIIQGMSISAREGASLEKLMQIARTTLRLWPELVK"
     gene            complement(9292..9831)
                     /locus_tag="PN977_002420"
     CDS             complement(9292..9831)
                     /locus_tag="PN977_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HDI6042443.1"
                     /translation="MNKSMLAGIGIGVAAALGVAAVASLNVFERGPQYAQVVSATPIK
                     ETVKTPRQECRNVTVTHRRPVQDENRITGSVLGAVAGGVIGHQFGGGRGKDVATVVGA
                     LGGGYAGNQIQGSLQESDTYTTTQQRCKTVYDKSEKMLGYDVTYKIGDQQGKIRMDRD
                     PGTQIPLDSNGQLILNNKV"
     gene            complement(10058..11362)
                     /gene="ndh"
                     /locus_tag="PN977_002421"
     CDS             complement(10058..11362)
                     /gene="ndh"
                     /locus_tag="PN977_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017384716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone dehydrogenase"
                     /protein_id="HDI6042444.1"
                     /translation="MTTPLKKIVIVGGGAGGLEMATQLGHKQGRKKKAKITLVDRNHS
                     HLWKPLLHEVATGSLDEGVDALSYLAHARNHGFQFQLGSVIDIDREAKTITIAELRDE
                     KGELLVPERKIAYDTLVMALGSTSNDFNTPGVKENCIFLDNPHQARRFHQEMLNLFLK
                     YSANLGANGKVNIAIVGGGATGVELSAELHNAVKQLHSYGYKGLTNEALNVTLVEAGE
                     RILPALPPRISAAAHSELTKLGVRVLTQTMVTSADEGGLHTKDGEYIEADLMVWAAGI
                     KAPDFLKDIGGLETNRINQLVVEPTLQTTRDPDIYAIGDCASCPRPEGGFVPPRAQAA
                     HQMATCAMNNILAQMNGKPLKSYQYKDHGSLVSLSNFSTVGSLMGNLTRGSMMIEGRI
                     ARFVYISLYRMHQIALHGYFKTGLMMLVGSINRVIRPRLKLH"
     gene            complement(11762..12304)
                     /locus_tag="PN977_002422"
     CDS             complement(11762..12304)
                     /locus_tag="PN977_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDI6042445.1"
                     /translation="MIIYLHGFDSNSPGNHEKVLQLQFIDPDVRLISYSTRHPKHDMQ
                     HLLKEVDKMLQLNVDERPLICGVGLGGYWAERIGFLCDIRQVIFNPNLFPYENMEGKI
                     DRPEEYADIATKCVTNFREKNRDRCLVILSRNDEALNSQRTSEELHHYYEIVWDEEQT
                     HKFKNISPHLQRIKAFKTLG"
     gene            complement(12327..13352)
                     /gene="nagZ"
                     /locus_tag="PN977_002423"
     CDS             complement(12327..13352)
                     /gene="nagZ"
                     /locus_tag="PN977_002423"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HDI6042446.1"
                     /translation="MGPVMLDVEGYELDAEEREILAHPLVGGLILFTRNYHDPAQLRE
                     LVRQIRAASRNHLVVAVDQEGGRVQRFREGFTRLPAAQSFAALLGMEEGGKLAQEAGW
                     LMASEMIAMDIDISFAPVLDVGHISAAIGERSYHADPEKALAIASRFIDGMHEAGMKT
                     TGKHFPGHGAVTADSHKETPCDPRPQAEIRAKDMSVFSSLIRENKLDAIMPAHVIYSD
                     VDPRPASGSPYWLKTVLRQELGFDGVIFSDDLSMEGAAIMGSYAERGQASLDAGCDMI
                     LVCNNRKGAVSVLDNLSPIKAERVTRLYHKGSFSRQELMDSARWKAISARLNQLHERW
                     QEEKAGH"
     gene            complement(13363..14187)
                     /gene="thiK"
                     /locus_tag="PN977_002424"
     CDS             complement(13363..14187)
                     /gene="thiK"
                     /locus_tag="PN977_002424"
                     /EC_number="2.7.1.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine kinase"
                     /protein_id="HDI6042447.1"
                     /translation="MPFRSNNPLTRDELLSRFFPQFHPVTTFNSGLSGGSFLIEHQGQ
                     RFVVRQPHDPDAPQSAFLRQYRALSQLPACIAPKPHLYLRDWMVVDYLPGEVKTYLPD
                     TNELAGLLYYLHQQPRFGWRITLLPLLELYWQQSDPARRTVGWLRRLKRLHKAREPRP
                     LRLSPLHMDVHAGNLVHSASGLKLIDWEYAGDGDIALELAAVWVENTDQHRQLVNDYA
                     TRAKIYPAQLWRQVRRWFPWLLMLKAGWFEYRWRQTGDQQFIRLADDTWRQLLIKQ"
     gene            complement(14168..14809)
                     /gene="lpoB"
                     /locus_tag="PN977_002425"
     CDS             complement(14168..14809)
                     /gene="lpoB"
                     /locus_tag="PN977_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoB"
                     /protein_id="HDI6042448.1"
                     /translation="MTKMSRYALITALAMFLAGCVGQREPAPVEEVKPAPEQPAEPQQ
                     PVPTVPSVPTIPQQPGPIEHEDQTAPPAPHIRHYDWNGAMQPMVSKMLGADGVTAGSV
                     LLVDSVNNRTNGSLNAAEATETLRNALANNGKFTLVSAQQLSMAKQQLGLSPQDSLGT
                     RSKAIGIARNVGAHYVLYSSASGNVNAPTLQMQLMLVQTGEIIWSGKGAVSQQ"
     gene            complement(14823..15200)
                     /locus_tag="PN977_002426"
     CDS             complement(14823..15200)
                     /locus_tag="PN977_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcfL family protein"
                     /protein_id="HDI6042449.1"
                     /translation="MRKGCFGLVSLALLVLLGCRSHPEIPVNDEQSLVMESSLLAAGI
                     SAEKPVLSTSDIQPSASSTLYNERQEPVTVHYRFYWYDARGLEMHPLERPRSVTIPAH
                     SAVTLYGSANFLGAHKVRLYLYL"
     gene            complement(15203..15562)
                     /gene="hinT"
                     /locus_tag="PN977_002427"
     CDS             complement(15203..15562)
                     /gene="hinT"
                     /locus_tag="PN977_002427"
                     /EC_number="3.9.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415621.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside phosphoramidase"
                     /protein_id="HDI6042450.1"
                     /translation="MAEETIFSKIIRREIPSDIVYQDDLVTAFRDISPQAPTHILIIP
                     NILIPTVNDVSAEHEQALGRMITVAAKIAEQEGIAEDGYRLIMNTNRHGGQEVYHIHM
                     HLLGGRPLGPMLAHKGL"
     gene            15896..18085
                     /gene="fhuE"
                     /locus_tag="PN977_002428"
     CDS             15896..18085
                     /gene="fhuE"
                     /locus_tag="PN977_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric-rhodotorulic acid/ferric-coprogen
                     receptor FhuE"
                     /protein_id="HDI6042451.1"
                     /translation="MLSTQFNRDNQHQAIIKSSLLAGCIALALLPSAAFAAPVTEETV
                     IVEGSATAPDDGENDYNVTSTSAGTKMQMTQRDIPQSVTIVSQQRMEDQQLQTLGEVM
                     ENTLGISKSQADSDRALYYSRGFQIDNYMVDGIPTYFESRWNLGDALSDMALFERVEV
                     VRGATGLMTGTGNPSAAINMVRKHATSREFKGDVSAEYGSWNKERYVADLQSPLTEDG
                     KIRARIVGGYQNNDSWLDRYNSEKTFFSGIVDADLGDLTMLSAGYEYQRIDVNSPTWG
                     GLPRWNTDGSSNSYDRARSTAPDWAYNDKEINKVFMTLKQRFADTWQATLNATHSEVE
                     FDSKMMYVDAYVNKADGMLVGPYSNYGPGFDYVGGTGWNSGKRKVDALDLFADGSYEL
                     FGRQHNLMFGGSYSKQNNRYFSSWANIFPDEIGSFYNFNGNFPQTDWSPQSLAQDDTT
                     HMKSLYAATRVTLADPLHLILGARYTNWRVDTLTYSMEKNHTTPYAGLVFDINDNWST
                     YASYTSIFQPQNDRDSSGKYLTPITGNNYELGLKSDWMNSRLTTTLAIFRIEQDNVAQ
                     STGTPIPGSNGETAYKAVDGTVSKGVEFELNGAITDNWQLTFGATRYIAEDNEGNAVN
                     PNLPRSTIKMFTSYRLPVMLELTVGGGVNWQNRVYTDTVTPYGTFRAEQGSYALVDLF
                     TRYQVTKNFSLQGNVNNLFDKTYDTNVEGSIVYGAPRNFSITGTYQF"
     gene            complement(18145..19578)
                     /gene="ptsG"
                     /locus_tag="PN977_002429"
     CDS             complement(18145..19578)
                     /gene="ptsG"
                     /locus_tag="PN977_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000475716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIBC"
                     /protein_id="HDI6042452.1"
                     /translation="MFKNAFANLQKVGKSLMLPVSVLPIAGILLGVGSANFSWLPAVV
                     SHVMAEAGGSVFANMPLIFAIGVALGFTNNDGVSALAAVVAYGIMVKTMAVVAPLVLH
                     LPAEEIASKHLADTGVLGGIISGAIAAYMFNRFYRIKLPEYLGFFAGKRFVPIISGLA
                     AIFTGVVLSFIWPPIGSAIQTFSQWAAYQNPVVAFGIYGFIERCLVPFGLHHIWNVPF
                     QMQIGEYTNAAGQVFHGDIPRYMAGDPTAGKLSGGFLFKMYGLPAAAIAIWHSAKPEN
                     RAKVGGIMISAALTSFLTGITEPIEFSFMFVAPILYIIHAILAGLAFPICILLGMRDG
                     TSFSHGLIDFIVLSGNSSKLWLFPIVGIGYAIVYYTIFRVLIKALDLKTPGREDATED
                     AKATGTSEMAPALVAAFGGKENITNLDACITRLRVSVADVSKVDQAGLKKLGAAGVVV
                     AGSGVQAIFGTKSDNLKTEMDEYIRNH"
     gene            complement(19873..20670)
                     /locus_tag="PN977_002430"
     CDS             complement(19873..20670)
                     /locus_tag="PN977_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HDI6042453.1"
                     /translation="MFLVDSHCHLDGLDYESLHKDVDDVLAKAAARDVKFCLAVATTL
                     PGYLHMRDLVGERDNVVFSCGVHPLNQNDPYDVEDLRRLAAEEGVVALGETGLDYYYT
                     PETKVRQQESFIHHIQIGRELNKPVIVHTRDARADTLAILREEKVTDCGGVLHCFTED
                     RETAGKLLDLGFYISFSGIVTFRNAEQLRDAARYVPLDRLLVETDSPYLAPVPHRGKE
                     NQPAMVRDVAEYMAVLKGVAVEELAQVTTDNFARLFHIDASRLQSIR"
     gene            complement(20681..21685)
                     /gene="holB"
                     /locus_tag="PN977_002431"
     CDS             complement(20681..21685)
                     /gene="holB"
                     /locus_tag="PN977_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HDI6042454.1"
                     /translation="MRWYPWLRPDFEKLVASYQAGRGHHALLIQALPGMGEDALIYAL
                     SRYLLCQQPQGHKSCGHCRGCQLMQAGTHPDYYTLAPEKGKNALGIDAVREVTEKLNE
                     HARLGGAKVVWVTDAALLTDAAANALLKTLEEPPAETWFFLATREPERLLATLRSRCR
                     LHYLAPPPEQYAVTWLSREVTMSQDALLAALRLSAGSPGAALALFQGDNWQARETLCQ
                     ALAYSVPSGDWYSLLAALNHEQAPARLHWLATLLMDALKRHHGAAQVTNVDVPGLVVE
                     LANHLSPSRLQAILGDVCHIREQLMSVTGINRELLITDLLLRIEHYLQPGVVLPVPHL
                     "
     gene            complement(21682..22323)
                     /locus_tag="PN977_002432"
     CDS             complement(21682..22323)
                     /locus_tag="PN977_002432"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HDI6042455.1"
                     /translation="MRSKYIVIEGLEGAGKTTARNVVVETLEQLGIRDMVFTREPGGT
                     QLAEKLRSLVLDIKSVGDEVITDKAEVLMFYAARVQLVETVIKPALANGTWVIGDRHD
                     LSTQAYQGGGRGIDQHMLATLRDAVLGDFRPDLTLYLDVTPEVGLKRARARGELDRIE
                     QESFDFFNRTRARYLELAAQDKSIHTIDATQPLESVMDAIRTTVTNWVKELDA"
     gene            complement(22313..23335)
                     /gene="yceG"
                     /locus_tag="PN977_002433"
     CDS             complement(22313..23335)
                     /gene="yceG"
                     /locus_tag="PN977_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein YceG"
                     /protein_id="HDI6042456.1"
                     /translation="MKKVLLIILLLLVVLGIAAGVGVWKVRHLADSKLLIKEETIFTL
                     KAGTGRLALGEQLYADKIINRPRVFQWLLRIEPDLSHFKAGTYRFTPQMTVREMLKLL
                     ESGKEAQFPLRLVEGMRLSDYLKQLREAPYIKHTLSDDKYATVAQALELENPEWIEGW
                     FWPDTWMYTANTTDVALLKRAHKKMVKAVDSAWEGRADGLPYKDKNQLVTMASIIEKE
                     TAVASERDQVASVFINRLRIGMRLQTDPTVIYGMGERYNGKLSRADLETPTAYNTYTI
                     TGLPPGAIATPGADSLKAAAHPAKTPYLYFVADGKGGHTFNTNLASHNKSVQDYLKVL
                     KEKNAQ"
     gene            complement(23338..24147)
                     /gene="pabC"
                     /locus_tag="PN977_002434"
     CDS             complement(23338..24147)
                     /gene="pabC"
                     /locus_tag="PN977_002434"
                     /EC_number="4.1.3.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate lyase"
                     /protein_id="HDI6042457.1"
                     /translation="MFLINGHKQESLAVSDRATQFGDGCFTTARVIDGKVSLLSAHIQ
                     RLQDACQRLMISCDFWPQLELEMKTLAAEQQNGVLKVVISRGSGGRGYSTLNSGPATR
                     ILSVTVYPAHYDRLRNEGMTLALSPVRLGRNPHLAGIKHLNRLEQVLIRSHLEQTNAD
                     EALVLDSEGWVTECCAANLFWRKGNVVYTPRLDQAGVNGIMRQFCIRLLAQSSYQLVE
                     VQASLEEALQADEMVICNALMPVMPVRACGDVSFSSATLYEYLAPLCERPN"
     gene            complement(24267..25508)
                     /gene="fabF"
                     /locus_tag="PN977_002435"
     CDS             complement(24267..25508)
                     /gene="fabF"
                     /locus_tag="PN977_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HDI6042458.1"
                     /translation="MSKRRVVVTGLGMLSPVGNTVESTWKALLAGQSGISLIDHFDTS
                     AYATKFAGLVKDFNCEDIISRKEQRKMDAFIQYGIVAGVQAMQDSGLEITEENATRIG
                     AAIGSGIGGLGLIEENHTSLMNGGPRKISPFFVPSTIVNMVAGHLTIMYGLRGPSISI
                     ATACTSGVHNIGHAARIIAYGDADVMVAGGAEKASTPLGVGGFGAARALSTRNDNPQA
                     ASRPWDKERDGFVLGDGAGMLVLEEYEHAKKRGAKIYAELVGFGMSSDAYHMTSPPEN
                     GAGAALAMANALRDAGIEASQIGYVNAHGTSTPAGDKAEAQAVKTIFGEAASRVLVSS
                     TKSMTGHLLGAAGAVESIYSILALRDQAVPPTINLDNPDEGCDLDFVPHEARQVSGME
                     YTLCNSFGFGGTNGSLIFKKI"
     gene            complement(25596..25832)
                     /gene="acpP"
                     /locus_tag="PN977_002436"
     CDS             complement(25596..25832)
                     /gene="acpP"
                     /locus_tag="PN977_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HDI6042459.1"
                     /translation="MSTIEERVKKIIGEQLGVKQEEVTNNASFVEDLGADSLDTVELV
                     MALEEEFDTEIPDEEAEKITTVQAAIDYINGHQA"
     gene            complement(26043..26777)
                     /gene="fabG"
                     /locus_tag="PN977_002437"
     CDS             complement(26043..26777)
                     /gene="fabG"
                     /locus_tag="PN977_002437"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HDI6042460.1"
                     /translation="MNFEGKIALVTGASRGIGRAIAETLAARGAKVIGTATSENGAQA
                     ISDYLGANGKGLMLNVTDPASIESVLEKIRAEFGEVDILVNNAGITRDNLLMRMKDEE
                     WNDIIETNLSSVFRLSKAVMRAMMKKRHGRIITIGSVVGTMGNGGQANYAAAKAGLIG
                     FSKSLAREVASRGITVNVVAPGFIETDMTRALSDDQRAGILAQVPAGRLGGAQEIANA
                     VAFLASDEAAYITGETLHVNGGMYMV"
     gene            complement(26790..27719)
                     /gene="fabD"
                     /locus_tag="PN977_002438"
     CDS             complement(26790..27719)
                     /gene="fabD"
                     /locus_tag="PN977_002438"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HDI6042461.1"
                     /translation="MTQFAFVFPGQGSQTVGMLADMAASYPIVEETFAEASAALGYDL
                     WALTQQGPAEELNKTWQTQPALLTASVALYRVWQQQGGKAPAMMAGHSLGEYSALVCA
                     GVIDFADAVRLVEMRGKFMQEAVPEGTGAMAAIIGLDDASIAKACEEAAEGQVVSPVN
                     FNSPGQVVIAGHKEAVERAGAACKAAGAKRALPLPVSVPSHCALMKPAADKLAVELAK
                     ITFNAPTVPVVNNVDVKCETNGDAIRDALVRQLYNPVQWTKSVEYMAAQGVEHLYEVG
                     PGKVLTGLTKRIVDTLTASALNEPSAMAAALEL"
     gene            complement(27735..28688)
                     /gene="fabH"
                     /locus_tag="PN977_002439"
     CDS             complement(27735..28688)
                     /gene="fabH"
                     /locus_tag="PN977_002439"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HDI6042462.1"
                     /translation="MYTKIIGTGSYLPEQVRTNADLEKMVDTSDEWIVTRTGIRERHI
                     AAPNETVSTMGFEAATRAIEMAGIEKDQIGLIVVATTSATHAFPSAACQIQSMLGIKG
                     CPAFDVAAACAGFTYALSVADQYVKSGAVKYALVVGSDVLARTCDPTDRGTIIIFGDG
                     AGAAVLAASEEPGIISTHLHADGSYGELLTLPNADRVNPENSIHLTMAGNEVFKVAVT
                     ELAHIVDETLAANNLDRSQLDWLVPHQANLRIISATAKKLGMSMDNVVVTLDRHGNTS
                     AASVPCALDEAVRDGRIKPGQLVLLEAFGGGFTWGSALVRF"
     gene            complement(28756..>29658)
                     /gene="plsX"
                     /locus_tag="PN977_002440"
     CDS             complement(28756..>29658)
                     /gene="plsX"
                     /locus_tag="PN977_002440"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HDI6042463.1"
                     /translation="RSRLQIIPAQSVIASDARPSQAIRASRGSSMRVALELVKEGRAQ
                     ACVSAGNTGALMGLAKLLLKPLEGIERPALVTVLPHQQKGKTVVLDLGANVDCDSTML
                     VQFAIMGSVLAEEVVEIPNPRVALLNIGEEEVKGLDSIRDASAVLKTIPSINYIGYLE
                     ANELLTGKTDVLVCDGFTGNVTLKTMEGVVRMFLSLLKSQGEGKKRSWWLLLLKRWLQ
                     KSLTRRFSHLNPDQYNGACLLGLRGTVIKSHGAANQRAFAVAIEQAVQAVQRQVPQRI
                     AARLESVYPAGFELLDGGKSGTLR"
BASE COUNT         7240 a         8007 c         7325 g         7086 t
ORIGIN      
        1 ccgagggcaa tgccgatggt agaaatgacg gagatcagcg acaccatgcc gccgcgccgc
       61 cgtccgcgac taaaacgcag gccaattaat aacgataaag gcatcgccat tactccgccc
      121 ccatcaggct cagttccgcc gtcagacgac catcacgcat ctccagttgg cggctcatac
      181 gtttcgccag ttgcaggtcg tgagtaacca ccaggaaggc ggtgccctgc aagcgattca
      241 attccccaag caactggaag atgctgtcgg cgttacgcgc atcgaggtta ccggtaggtt
      301 catccgccag taccagacgc gggttattga ccagcgcacg ggcaatcgcc acacgctggc
      361 gttcgccgcc agaaagttca gatgggcggt gattcgcacg atgccccagc cccaccgctt
      421 ttaacatctc cagcgcacgg ctgttgattt cagcgggctt tttcttgcca atcagcagtg
      481 gcatagccac gttttccagg gcagtaaaat ccggcagcag gtggtgaaac tgataaataa
      541 agcccagctt ctggttgcgc agttctgctt ttgccgccga agacagtttg ctcatcggtt
      601 gaccgttaaa gatcacatcg ccggaggttg gtgtatccag cccgcccagc aggtgcagca
      661 aggtactttt accggaacca gagctaccga cgatcgccat catttcacct tcgccgacgc
      721 tgaaactgac attgtgcagt acatcggttt gcacactgcc ttcctgatag cgtttgcaca
      781 ggttgtcgca ttgcaacagg atcttattca taacgtaaag cctcagcggg ttgagtggcg
      841 gcagcgcgcc atgaagggta aagcgtagac agcagcgcga tagccatcgc caccagcgca
      901 ataacaatga cctgtaaagg ttcgatagcg accggcagcg ccgcgccatc aagcaggacg
      961 ccgattatcg gcatcagatt attcaactgg ctggcaagca gtgcgccaag cgccgctccg
     1021 aggatcgcac cgataatccc agcgctggcc ccttgcacca taaagaccat catgatttgt
     1081 cgcggagtta agccttgcgt ttgcaggatc gctacttcgc cctgcttctc catcaccatc
     1141 agccctagcg aggtaataat gttaaacgcc gcaacggcga caatcaggct cagcagtaag
     1201 cccatcatat ttttttccat gcgtacggcc tggaaaagct cgcctttacg gtcacgccag
     1261 tcctgccatt tgctgccttc aggcagtttt tgctgactta atgagtcgac tttcagcggc
     1321 tcatccagcc acaaacgcca gccggtaata ttgcctgccg gataacgcat cagacgcgag
     1381 gcatcctcaa tattcaccag catttcatag ccatcgactt cactgttggc ggcgaaagtg
     1441 ccaatcacat tgaacaggcg ctggcttgga atacgcccca tcggcgtgaa ctggctggca
     1501 gatggcacca tcacgcggat ttgatcaccg cgattgacgc ccaactgtga ggcaagctgt
     1561 tcaccgagga tgacattata tttccccggc tcgaggtcag tttgtttcac attgaccaga
     1621 tacggcgtta gtggatcttt ttgcgccggg tcgataccca gcatcacccc gaccgccacg
     1681 ctgcgcgcgc tttgcagtac cacatcaccg gtagtaatag gtgcgacgcg attaacgccg
     1741 tccagtttga ctgccgtttc cgggagttgc tgcgggttaa gagagccatg ctcagaagag
     1801 agaattgcct gtggcatcag gccaaggatg ttgttttgca gctcgcgctc aaagccgttc
     1861 atcactgaca atactgtgac cagcgccatc accccgaggg taatgccgat ggtagaaagc
     1921 caggagacga aacgaccgaa gcgatccgct gcacgcccac gcatgtaacg caggccaata
     1981 aatagagcga caggttggta catgaaatcc gtctggttgc tgtagcaaag acacgagtat
     2041 ataagcgaaa gcgggatggg taaacgtctg aactgtaagt gatcgtcagg gctaatctga
     2101 aaagtaccag caaatcaatc gactaaaggg cctcattttc tcattggcct tgtggcctga
     2161 tctttatctg aaaaataacc aaatacagac agatacgcat tacaccacgc gactggcccc
     2221 tcaggatcaa cagcatgtta aataaaaaga gcgtgttgtc ctgatgaaac aaaaagagct
     2281 atggattaac cagatcaaag ggttatgtat ttgtctggta gtgatttatc actcggtgat
     2341 taccttttat ccgcatctga gcacttttca gcatccgtta tcggaagtcc tgagcaaatg
     2401 ctggatctat ttcaatcttt accttgcccc ctttcgtatg ccggtttttt tctttatctc
     2461 tggctattta attcgccgct atatcgacag cgtgccatgg ggaaattgtc ttgataaacg
     2521 catctggagc atcttctggg tgctggcact ttggggcgta gtgcagtggc tggcgataag
     2581 tgcactaaat cagtggctgg cacctgagcg caatttaagt aatgcctcca atgccgctta
     2641 tgccgattct accggtgagt tcctgcacgg gatgatcacc gccagcacca gcttgtggta
     2701 tctgtatgct ttaattgtct atttcgtgat atgtaaaatt tttaaccgcc tggcgctgcc
     2761 actattcgcc ttgtttgtac tgctgagtgt ggcggttaat ttcgttccca cgccgtggtg
     2821 gggaatgaac agtgtgatcc gcaatttgcc ttattacagc cttggcgcat ggtttggcgc
     2881 aacaataatg acctgtgtta aagaggtgcc gttgcgccgc catctgctga tggcttcttt
     2941 gctgaccgtt ctggcggtcg gtgcctggtt gtttactatc tcgctgctgt tgtcgctggt
     3001 atcgattgtg gtaatcatga agctgtttta tcagtacgag cagcgtttcg gtatgcgttc
     3061 ctccagcctg ctgaatgtga ttggttccaa caccattgct atctacacca cccatcgcat
     3121 tctggttgaa atattcagct taactctgct tgcgcaaatg aacgcagcac gctggtcgcc
     3181 gcaagtcgaa ctgacactcc tgctggttta cccctttgtt agtttgttca tctgtactgt
     3241 tgcgggcttg ctggtaagaa aactttcaca gcgcgcattc agcgatctgt tgttctcccc
     3301 gccttctctg cccgcggccg tcagttactc ccgctaagcc atcgccccag ccggggcgat
     3361 tcaatttgct aaaccatgtc gattacggat aatgaacagc attgcttatc agagatatgc
     3421 ccccatatgt tgaggcatat cctaacgaga atctgacaac cgttatgcct gaacaatatc
     3481 gttatacgct gcccgtcaaa gcgggtgagc agcgtctgct gggcgagtta actggcgctg
     3541 cctgcgcgac gctggtagcg gaaattgccg aacgtcacgc cggaccagtg gtgcttattg
     3601 cgccagatat gcaaaatgct ctgcgtttgc atgatgaaat tagccagttt accgaccaga
     3661 tggtgatgaa tctggcggac tgggaaactc ttccctacga cagtttttcg cctcatcagg
     3721 acattatctc ctcgcgcctt tccacccttt accagctacc gacgatgcag cgtggcgtac
     3781 tgattgttcc ggtgaatacg cttatgcagc gcgtttgccc gcacagtttt ctccacggcc
     3841 atgcgctggt gatgaaaaaa ggtcagcgcc tgtcacgaga tgcattacga acccaactgg
     3901 acagcgccgg ttatcgccat gttgaccagg tgatggagca cggcgaatac gccacgcgcg
     3961 gcgcgttgct ggatctcttc ccgatgggga gtgagctgcc ttatcgtctt gatttctttg
     4021 atgatgaaat cgacagcctg cgggtgtttg acgtcgacag ccagcgcacg ctggaggaag
     4081 tagaagcgat caatctgctg cccgcgcacg aatttccgac cgataaagcg gcaattgaac
     4141 tgttccgcag ccagtggcgc gataccttcg aagtgaagcg cgatccggaa catatttacc
     4201 agcaagtgag taaaggcaca ttacctgccg ggatcgagta ctggcagccg ctattcttca
     4261 gcgaaccact gccgccgctg ttcagttatt tccctgccaa taccttgctg gtgaatactg
     4321 gcgatctgga aaacagtgcc gaacgtttcc aggctgacac gctggcgcgt tttgagaatc
     4381 gcggcgtcga tccgatgcgc ccactgttgc caccacaatc gctctggctg cgggtggacg
     4441 agctcttctc agagctgaaa aactggcccc gagtgcagct aaaaactgaa catttaccga
     4501 caaaagccgc gaatgccaat ttaggtttcc agaaactgcc agacctggcc gttcaggcac
     4561 aacaaaaagc accgctggat gcgctgcgta agttcctcga gtctttcgac ggtccggtgg
     4621 tgttctcggt agaaagtgaa ggtcgccgtg aagcgctggg tgaactgctc gcgcgaatta
     4681 aaattgctcc tcaacgcatt atgcgtcttg atgaagccaa agaccgtggg cgttatctga
     4741 tgattggcgc tgccgaacat ggttttgtcg atacggtgcg taatctggcg ctgatttgcg
     4801 aaagcgatct gctcggtgaa cgtgttgccc gtcgtcgtca ggactctcgc cgcaccatca
     4861 accccgatac actgatccgt aacctcgcgg agctgcatat tggtcagccg gtggtccatc
     4921 tggagcacgg tgtcggccgc tatgccggaa tgaccacgct cgaagcgggc ggcattactg
     4981 gcgagtattt gatgctcacc tatgccaacg acgccaaact gtatgttccg gtgtcgtcac
     5041 tgcatctgat tagccgttac gcgggtggcg cggaagaaaa cgccccgctg cataaacttg
     5101 gcggcgatgc gtggtcacgc gctcggcaga aagcggcgga aaaagtgcgt gatgtggcag
     5161 cagaattgct ggatatctac gcgcaacgag ccgccaaaga gggcttcgcg tttaaacacg
     5221 atcgtgagca gtatcagttg ttctgcgaca gcttcccgtt tgaaaccacg ccggatcagg
     5281 cacaggccat taatgcggta cttagcgaca tgtgtcagcc gctggcaatg gatcgtctgg
     5341 tgtgcggcga tgttggcttt ggtaaaacag aagtggcgat gcgcgccgct ttcctggcag
     5401 tagataacca caagcaggtg gcggtgctgg tgcctaccac ccttctcgcg cagcagcatt
     5461 acgacaactt ccgcgaccgt ttcgctaact ggccagtacg catcgaaatg atctcccgtt
     5521 tccgtagcgc caaagagcag acgcaaatcc ttgcggaagt ggcggaaggg aaaatcgata
     5581 ttctgatcgg tacgcacaaa ctgctgcaaa atgacgtcaa gtttaaagat ttaggtctgc
     5641 tgattgtcga tgaagaacac cgcttcgggg tgcgtcataa agagcgcatt aaagcgatgc
     5701 gggcgaacgt ggatattctg acacttaccg caacgccgat cccacgtacg ctgaatatgg
     5761 caatgagcgg aatgcgtgac ctgtcgatta tcgccacgcc gcccgcccgt cgtctggcag
     5821 ttaaaacctt tgtccgtgag tatgacagca tggtggtcag ggaggcgatc ctgcgtgaaa
     5881 ttttgcgcgg ggggcaggtt tattatcttt acaatgatgt ggaaaacatc cagaaagccg
     5941 ccgaacggct ggcagaactg gtgccagaag cgcggatcgc catcggtcac gggcaaatgc
     6001 gtgagcgcga actggaacgg gtgatgaatg atttccatca tcaacgtttc aacgtgctgg
     6061 tttgtaccac cattatcgaa accgggatcg acatcccgac agccaacacc attatcattg
     6121 aacgcgcgga tcacttcggt ctggcgcagc tgcaccagtt acgcggtcgc gtcggacgtt
     6181 cgcatcatca ggcatatgca tggttgctga caccgcatcc aaaagcgatg actaccgatg
     6241 cacaaaaacg tcttgaagcg attgcctcgc tggaagatct cggtgcaggt tttgcgctgg
     6301 caacgcacga tctggagatc cgcggcgcgg gtgaactgct tggcgaagaa caaagcggct
     6361 caatggaaac catcggtttc tcgctgtata tggagttgct ggaaaacgcc gtcgatgcgc
     6421 tgaaagccgg acgcgagccg tcgctggaag atctcaccag ccagcaaaca gaagtcgagc
     6481 tgcggatacc gtcgctattg ccagatgatt tcattcctga cgtgaatacg cgtttgtcgt
     6541 tctataaacg tattgccagc gccaaaacgg aaaacgaact ggaagagatc aaagtcgagc
     6601 ttatcgatcg cttcggcctg ctgccggatc cggcgcgtac cctgctggat attgcccgtc
     6661 tgcgccagca agcgcagaaa ctggggatca ggaagctgga aggtaatgag aaaggcggcg
     6721 tgatcgaatt tgccgagaag aatcacgtta atccggcctg gttgattggt ttgctgcaaa
     6781 aacagccgca gcattatcgc ctcgatggcc cgacgcgcct gaagtttatt caggatttga
     6841 gtgagcggaa aacgcgtatc gaatgggtac gccagtttat gcgtgaactg gaagagaacg
     6901 cgatcgcttg atggttagat taggggcgtt tctgcgcccc catctgcaac atttacaaat
     6961 tctttgcact tccctgcact atccgacact gtcaccatcc ataattcagg cttatctgtt
     7021 tattacaata accttatatt tattatggat ttttggtgat gatcaaaacg catttttctc
     7081 gctggctaac gttttttacg ttcgccgctg ccgtggcgct ggcgctaccg gcaaaagcca
     7141 acacctggcc gctgccgcct gcgggcagtc gtctggttgg tgaaaacaaa tttcatgttg
     7201 tggaaaatga cggtggttct ctggaagcca tcgccaaaaa atataacgtc ggctttctcg
     7261 cgctgttaca ggctaacccc ggcgttgatc cttacgtacc gcgcgcgggt agcgtgttaa
     7321 cgatcccgtt gcaaacccta cttccagacg cgccgcgtga aggcattgtg atcaacattg
     7381 cggagctgcg tctctattac tacccgccgg gtaaaaattc ggtaaccgtg tatcccatcg
     7441 gcattggtca gttgggtggt gacacgctga caccaacaat ggtgaccacc gtttcagaca
     7501 aacgtgcaaa cccaacctgg acgccaacgg caaacatccg cgcccgttat aaagcacagg
     7561 gaattgagtt gcctgcggta gtgccggctg gaccggataa cccaatgggc catcatgcga
     7621 ttcgtctggc ggcctatggc ggcgtttatt tgcttcatgg tacgaacgcc gatttcggca
     7681 ttggcatgcg ggtaagttct ggctgtattc gtctgcggga tgacgatatc aaaacactct
     7741 ttagccaggt caccccaggc accaaagtga atatcatcaa cactccgata aaagtctctg
     7801 ccgaaccaaa cggtgcgcgt ctggttgaag tacatcagcc gctgtctgag aagattgatg
     7861 acgatccgca gctgctgcca attacgctga atagcgcaat gcaatcattt aaagatgcag
     7921 cacaaactga cgctgaagtg atgcaacatg tgatggatgt ccgttccggg atgccggtgg
     7981 atgtccgccg tcatcaagtg agcccacaaa ctctgtaaat cctggataaa aaaatccccg
     8041 cgacacggca tgtcgcgggg aaaaaatcag cagtagcagg cattaatgag ggttaatgct
     8101 tatttataaa ttactgctgt tccatggagg gtattcggac cggttacaga agtaatacgg
     8161 aaagattttg cgcccatctc atccgctttt tgcgccagct gctcttccag cgatcccaga
     8221 tttgtccccg cgttagcact gatagtaccg actttttgtt ggccttctgg cgttgactga
     8281 acttcgacag ccgcaaagct ggcaaatgac attgagctta aaatcgccgc agcgatgagg
     8341 gtttttacgt ttttcataat agtggcctta tgcagatgaa tgacgtcgca agcaattact
     8401 taacgatcga taactaaatg atagatgtga tctggatcac atacaagata ttttttataa
     8461 caatcattaa ttaatttgaa aatctttaaa tttcagttaa atagaaagca attattttta
     8521 tgggatctgg actggtgaac ggaggtgatt atttttataa taacctttca ttcacagtca
     8581 ccaggtacaa cggcatcatg gcaactgact caacacaatg tgtaaaaaaa agccgtggcc
     8641 gcccaaaagt gttcgacagg gatgccgcgc ttgataaggc catgaaattg ttctggcaac
     8701 acggttatga agcgacttct cttgcggacc tcgttgaagc gaccggagct aaagcgccca
     8761 cgctatacgc ggaatttacc aacaaagagg ggttattccg cgccgtactc gaccgctata
     8821 tcgatcgttt tgccgctaag catgaagcac agctgttttg tgaagagaaa agcgtggagt
     8881 ctgcgctggc tgactatttt gctgccatcg ccaactgctt taccagcaaa gacaccccgg
     8941 ctggctgctt tatgatcaac aactgcacca ccctctcccc ggattccgga gatatcggca
     9001 atacgctgaa atcacgccat gcgatgcaag agcgcacttt gcagcagttt ttatgtcaac
     9061 gacaagcgcg cggggaaatc ccgacccact gtgacgtgac gcatctggca gaattcctta
     9121 attgtattat tcaggggatg tcgatcagcg cacgcgaagg tgcatcgctg gaaaaactga
     9181 tgcagattgc ccgaacgact ttgcgtttat ggcccgaact ggtgaaataa ggcgcaaaaa
     9241 aagcccctca gcctgagcga atgaggggct aaattgcaga gtacagcctt gttatacttt
     9301 gttattcaaa atcagttgcc cgttgctatc tagcgggatc tgcgtacccg gatcgcggtc
     9361 catgcggatt ttgccctgct gatcgccaat cttataggtc acatcataac cgagcatttt
     9421 ttctgacttg tcatacaccg ttttacaacg ctgttgcgta gtcgtgtaag tatcgctttc
     9481 ctggagagag ccctggatct ggttaccggc atatccacca cccagcgccc ccacaacagt
     9541 agcgacatct ttaccgcgac caccaccaaa ctgatgccct atcacgccac cagcaacagc
     9601 gccgagcacc gacccggtaa tgcgattttc atcctgcacc ggtcgacgat gggtcactgt
     9661 gacgttgcga cactcctgac gcggtgtttt aaccgtttcc ttgattgggg ttgcagaaac
     9721 aacctgagcg tattgcgggc cacgttcaaa cacgttcaga ctggccactg ccgctacgcc
     9781 cagcgcagct gcgacaccaa tcccgatacc cgccaacatt gatttattca cgggaacacc
     9841 tccttctttg cagttatgcc aatcatgcaa gcgaaggaga aaagcagcta tcagacagaa
     9901 gatgaaaagc gcgagaatgg ctggaaatac ttaaggattc ggaggagtct gaaggaatgc
     9961 ctgatgcgac gcttaacgcg tcttatcagg cctaccgctc gtaccatttg tccagggcgg
    10021 atgaggcgtt tatgccccat ccgccagtac aagccgatta atgcaacttc aaacgtggac
    10081 ggataacgcg gttaatactc cccaccagca tcattaatcc ggttttaaag taaccatgca
    10141 gcgcaatctg atgcattctg tatagcgaga tgtatacaaa gcgcgcaatt cgtccttcaa
    10201 tcatcattga gccgcgcgtc aggttaccca tcaggctacc gacggtggag aagttcgaca
    10261 gcgataccag agaaccgtga tctttatact gataactttt cagcggctta ccgttcatct
    10321 gcgccagaat gttgttcatt gcgcaagttg ccatctggtg tgcagcctga gcgcgcggcg
    10381 gaacaaagcc cccttccgga cgcgggcatg acgcgcaatc gccaatagcg taaatgtctg
    10441 gatcgcgggt ggtttgcagc gtcggttcca ccaccagctg gttgatacgg ttggtttcaa
    10501 gaccaccgat atctttcagg aagtctggcg ctttgatccc ggctgcccac accatcagat
    10561 cagcctcaat atattcgcca tctttagtat gcaggccgcc ttcatcagca ctggtgacca
    10621 tggtttgcgt cagcacgcga acgccaagtt tcgttaactc actatgggcc gcagcagaga
    10681 tacgtggcgg caatgcaggc aaaatacgtt cgcccgcttc taccagcgtt acgttcaggg
    10741 cttcgttggt cagaccttta taaccgtagc tgtgcagttg cttgaccgcg ttgtgcaatt
    10801 cagcggagag ttctacaccc gtcgcgccgc cgccgacaat cgcaatgttc actttgccat
    10861 tcgcgcccag gttggcggag tatttcagaa acagattcag catctcctgg tggaagcgac
    10921 gcgcctggtg cgggttatcg aggaaaatgc agttctcttt gacacctggc gtattgaaat
    10981 cgttagaggt gctacccagc gccattacca gggtgtcata ggcgatttta cgttccggaa
    11041 ccagcaattc acctttttcg tcgcgcagtt ctgcaatagt gattgttttc gcttcacgat
    11101 cgatatcaat gacggaaccc agctggaact ggaaaccatg attgcgcgca tgggccagat
    11161 agctcaacgc atcgacgcct tcatcaagcg agccagtcgc cacttcgtgc agcagcggtt
    11221 tccacaagtg gctgtggtta cgatcgacca gcgtaatttt ggcttttttc ttgcgtccct
    11281 gcttatgtcc cagctgtgtc gccatttcca gcccaccagc accgccgccg acaatcacaa
    11341 tttttttcaa tggcgtagtc aacgtgaccc ccttaaattt attaaccaat tgttaactaa
    11401 tagttattaa catagctctt aattaacaac aggttacagc aatgaaaatg ttcttcgcta
    11461 atgagaataa catgaatggt gcattggtca taccaaaatt gatgttaatc aagttttgtt
    11521 gctgaaaaga taggcgactt taccgggtga gatcaaaaaa cagagtttgt tgtttattat
    11581 ttgagctgat ttgtgagaat gatatagagt gaggtgtgta tggagggcgt ttggtaggcc
    11641 tgataagaag cgcatcaggc attgatgctc gactgtcgga tgcggcgtga acgccttatc
    11701 cgacccacag aatccggagc gcatcaggta ccggcaaggt tacctgatgc gacgagggca
    11761 tttacccgag ggttttgaac gctttaatgc gctgtaaatg cggggagata ttcttgaatt
    11821 tgtgcgtctg ctcttcgtcc cagacaatct cgtaataatg atgtaactct tcagatgtcc
    11881 gctggctgtt aagcgcttca tcattacgcg acaaaatcac caggcaacga tcgcgattct
    11941 tctcacggaa gttggtcaca cacttagtgg caatatcggc atactcttcc gggcgatcaa
    12001 tcttcccttc catgttctca taagggaaca aattggggtt gaagatcacc tggcggatat
    12061 cacagagaaa accaatccgc tcagcccaat aaccgcccag accaacacca caaatgagcg
    12121 ggcgctcgtc gacgttcagt tgcaacattt tgtccacttc tttgagcaaa tgctgcatat
    12181 catgtttcgg atgccgcgta ctgtagctta ttagccgtac atccgggtca ataaactgca
    12241 attgtaagac tttctcgtgg ttacccggac tgttagagtc aaaaccgtgc aaatagataa
    12301 tcatcgcttc ctcacataag ccagggttaa tgacctgcct tctcttcctg ccagcgttca
    12361 tgtaactgat tcagacgggc gctgatcgct ttccagcgag ccgagtccat cagttcctgt
    12421 cgcgaaaatg aacctttatg atacaaacgt gtaacacgtt ctgccttgat cggtgacaga
    12481 ttatctaaca cgctgacggc ccctttacga ttattgcaga ccaggatcat atcgcaacct
    12541 gcatccagtg atgcctgtcc acgctcggca taactgccca taatcgctgc gccttccatc
    12601 gataaatcgt cagagaaaat cacgccgtca aaacccaatt cctgacgcaa aacggttttc
    12661 agccagtagg gagaaccgct cgccggacgc ggatcaacat cactgtagat cacatgcgca
    12721 ggcataatgg cgtcgagttt attttcgcgg attaaggaac tgaagaccga catatcttta
    12781 gcgcgaatct ccgcttgcgg acgtgggtcg cacggtgttt ctttgtgtga gtctgccgtt
    12841 actgcaccgt gacctgggaa gtgtttcccg gtagttttca ttccggcttc atgcatacca
    12901 tcaataaacc ggctggcaat tgccagggct ttttcgggat cggcatgata agaacgctcg
    12961 ccaatcgccg cgctgatatg cccgacgtcc agcactggcg caaagctgat atcaatatcc
    13021 atagcaatca tttcgctggc catcaaccaa cctgcctctt gcgccagttt tccgccttct
    13081 tccattccca acagcgcagc aaatgattgt gccgctggca agcgggtaaa accttcacga
    13141 aaacgctgca cgcgtccacc ttcctgatca accgccacca ccagatgatt gcgcgaagct
    13201 gcgcggatct ggcgcaccag ttcacgtaac tgggcaggat catgatagtt acgcgtaaag
    13261 agaatcagcc ctcccaccag cggatgcgcc agtatttcac gctcttccgc gtccagttcg
    13321 taaccttcga catccaacat tactggaccc acactgctct ccttattgtt ttattaatag
    13381 ctgccgccag gtgtcatcgg ccagcctgat aaattgttga tcgccggttt gtcgccagcg
    13441 gtactcaaac caccctgctt tgagcatcag cagccaggga aaccatcgcc tgacctgacg
    13501 ccataattgc gccggataaa tcttcgcgcg agtggcatag tcattgacca attgccggtg
    13561 ctggtcagta ttttccaccc acaccgccgc cagttccagc gcgatatcac catctccggc
    13621 atactcccag tcgatgagtt ttaaccctga cgcgctatgc actaaatttc cggcgtggac
    13681 atccatatgc aatggactta agcgtaaagg ccgtggttcc cgcgctttgt gcagacgttt
    13741 taaccttcgc agccaaccca ctgtccgccg cgccggatcg ctttgctgcc agtacagttc
    13801 cagtaacggc aacagcgtta ttcgccagcc aaaacgcggt tgttgatgta gataatacag
    13861 caagcctgcc agttcgttag tatccggcaa atacgttttt acctcgccgg gcagatagtc
    13921 gactaccatc cagtcacgga gatataaatg cggcttcggt gcaatgcatg cgggtagttg
    13981 tgataaagcc cgatactggc gcaagaacgc ggactgcggc gcatcaggat cgtgcggctg
    14041 acgcacaaca aaacgctggc cctgatgttc aatgagaaaa ctcccgccac taagcccact
    14101 attaaacgtc gtgacgggat gaaactgcgg gaaaaagcgc gacagcaatt cgtcgcgcgt
    14161 gaggggatta ttgctgcgaa acggcacctt tacctgacca gataatttcg cccgtctgca
    14221 ccagcatcag ctgcatttgt agggtcggag cgttaacgtt gccagaggcg ctggagtaca
    14281 gcacgtaatg agcgccaaca ttgcgggcaa tgcctatggc tttactacgg gtgcctaaac
    14341 tgtcctgcgg cgacaaacct aattgttgct tagccatcga cagctgctgg gcggaaacca
    14401 gggtaaattt accgttatta gccagcgcat ttcgcagcgt ttcggtcgct tctgcggcat
    14461 tcagcgaacc gttagtacgg ttgttaacgc tatcaaccag caggacgcta cccgcagtca
    14521 cgccatcagc cccaagcatc ttactgacca tcggctgcat tgcgccattc cagtcataat
    14581 ggcgaatatg cggcgcaggc ggtgcagttt gatcttcgtg ctcaattggg cctggctgct
    14641 gcggtatcgt cggcaccgat ggcactgtgg ggacaggctg ttgcggctcg gctggttgtt
    14701 ccggcgctgg tttcacttct tctaccggcg caggttcacg ttgccccaca cacccggcga
    14761 gaaacatcgc cagcgcggta atcaaggcgt agcgactcat ttttgtcatc aagattcacc
    14821 ccttacaaat atagataaag tctgactttg tgcgccccca gaaaattggc gctgccgtac
    14881 agcgtgaccg ccgaatgtgc gggaatggta acgctgcgtg gcctttccag aggatgcatc
    14941 tccagccctc tggcgtcata ccagtaaaaa cgataatgaa cggtaacggg ttcttgcctt
    15001 tcgttataaa gcgttgagga tgctgaaggt tgaatatcag atgtcgaaag gacgggcttt
    15061 tctgcactga tgcccgcagc caataaagat gactccatca ccagcgattg ctcatcattc
    15121 accggaattt ccggatgtga acgacagccc aacagcacta acaacgccag agacaccagc
    15181 ccaaagcatc cttttctcat cgttacagac ctttatgcgc cagcattggt ccaagcggac
    15241 ggccacccaa caagtgcata tggatgtggt aaacctcttg tccgccgtgg cggttggtgt
    15301 tcatgatcag acgatagcca tcttcggcaa taccttcttg ctcagcaatt tttgccgcta
    15361 cggtgatcat gcgtcccagc gcctgctcat gctcagcaga cacgtcgttc acagtcggaa
    15421 tgaggatatt cggaatgatc agaatatgcg ttggcgcttg cggcgaaata tcgcgaaacg
    15481 ccgttaccag atcatcctgg tagacgatat cggaggggat ctcacgacga ataattttgc
    15541 tgaatatagt ttcttctgcc acgacatttt cctttttcaa atagccatgc gttgcgccat
    15601 gctacgccgc gccacactgc gagtatagag tatggtcgag ttaaacctgc tctttcaacc
    15661 ttaactcacg atttcttaag caaaaaatcg ttttgctgcc gtatatatcg ccattattcc
    15721 catttctgcc tgcgaatgcg ctgaaaagac attctctgtt tggtttacac actaaatatg
    15781 aatgcgtata tttctcattt gcatttacat acaaaattat tcgcacacta aatataaccg
    15841 ctcatcaccc tgctccggca tgagcctgac gccaacataa accaagagat ttcaaatgct
    15901 ttcgacacaa tttaacaggg ataatcaaca tcaagctatc atcaaatcgt cactccttgc
    15961 cggttgcata gcactggcac tattaccttc cgccgctttt gctgcaccag tcactgaaga
    16021 aacggtgatt gttgagggtt cagccacagc cccagatgat ggcgaaaatg attacaacgt
    16081 aacgtctacc tctgcgggta caaaaatgca gatgactcaa cgtgatattc ctcagtcggt
    16141 cactattgtt agccagcagc ggatggaaga tcagcagtta caaacgctgg gcgaagtgat
    16201 ggaaaacacg ctggggatca gcaaaagtca ggcggattcc gatcgtgctc tttattattc
    16261 ccgcggattc cagatcgata actatatggt tgatggtatc cccacctatt ttgaatcgcg
    16321 ctggaatctg ggcgacgcac tttctgatat ggcactgttt gaacgcgtag aagtagtgcg
    16381 tggcgcgaca ggcctcatga ccgggacggg taatccatct gcggcaatta atatggttcg
    16441 aaaacacgcg accagtcgtg aatttaaagg cgatgtctcg gcggaatacg gtagctggaa
    16501 caaagaacgg tatgtggcgg atttacaaag cccactcacc gaagatggta aaatccgcgc
    16561 acgaattgtc ggcggctacc agaataacga ctcatggctg gaccgctaca acagtgaaaa
    16621 gaccttcttc tccggcattg tcgatgctga tttaggcgat cttacgatgc tttcagccgg
    16681 ttacgaatat cagcgtattg atgttaatag cccaacctgg ggcggtttac cgcgctggaa
    16741 tactgatggc agcagcaaca gttacgatcg cgcacgcagt accgcacctg actgggcgta
    16801 caacgataaa gagatcaaca aggtctttat gaccctgaag cagcggtttg ctgatacctg
    16861 gcaagcgacg ctgaatgcca cccactctga agtcgaattt gacagcaaaa tgatgtatgt
    16921 cgatgcctat gtaaacaaag cggatggtat gctggttggg ccatacagta attatggacc
    16981 tggttttgat tatgtcggcg gcaccggttg gaacagcggc aaacgtaaag ttgatgcgct
    17041 ggatttgttc gctgacggta gttatgaatt gtttggtcgt cagcacaatc taatgtttgg
    17101 tggcagttac agcaaacaaa acaatcgtta cttcagttca tgggccaaca tcttcccgga
    17161 tgaaattggc agtttctaca actttaatgg caatttccca caaaccgact ggtcaccaca
    17221 gagcctggcg caggacgata ccacacatat gaaatcgtta tatgctgcca ctcgtgtcac
    17281 ccttgccgat ccgctgcatc tgatcctcgg cgctcgttat accaactggc gggttgatac
    17341 gctgacttac agcatggaga aaaaccacac cacgccttat gctggtctgg tgtttgacat
    17401 caatgacaac tggtcgacct acgccagcta tacctctatt ttccagccgc aaaatgatcg
    17461 tgacagttca ggtaaatatc tgactccaat caccggtaac aactacgagt tgggtctgaa
    17521 atcggactgg atgaatagcc gtctgaccac cacgttagcc atcttccgta ttgagcagga
    17581 taatgtcgct cagtccaccg gtacacctat ccccggcagc aacggcgaaa ccgcctataa
    17641 agcggtggat gggacagtca gtaaaggtgt ggaatttgaa ctcaacggcg caattaccga
    17701 caactggcag ctgacgtttg gcgcaacgcg ctatattgca gaggataacg aaggaaacgc
    17761 cgttaatcct aatctgccac gctccacgat taaaatgttc accagctatc ggttgcctgt
    17821 catgctagag ttgacggtcg gcggtggtgt taactggcaa aatcgcgtgt ataccgacac
    17881 cgtgacaccg tatggcacct tccgcgccga gcaaggtagc tacgcgctgg tggatctctt
    17941 cacccgctac caggtgacga aaaacttctc gttacagggg aacgtcaata acctgttcga
    18001 caaaacctac gataccaacg tggaaggttc tatcgtctac ggcgcaccgc gtaatttcag
    18061 cattaccggc acgtatcaat tctgaataac acctgtaaaa aaggcagcca tctggctgcc
    18121 ttagtctccc caacgtctta cggattagtg gttacggatg tactcatcca tctcggtttt
    18181 caggttatcg gatttagtac cgaaaatcgc ctgaacacca gaaccagcaa ccactacgcc
    18241 cgctgcgccc agtttcttca ggccagcctg atccacttta gacacatcag caacgctgac
    18301 gcgcagacgg gtaatacatg cgtcgaggtt agtaatgttt tctttaccac caaatgcagc
    18361 aaccagagcc ggtgccattt cgctggtacc tgtcgctttt gcgtcttcag tcgcgtcttc
    18421 acgacccggc gttttcagat ccagtgcttt aatcagcacg cggaagatgg tgtagtaaac
    18481 aatcgcataa ccgataccga cgatcgggaa cagccacagt ttgctgctgt taccagacag
    18541 aacgatgaag tcgatcagac cgtgcgagaa cgacgtaccg tcacgcatcc ccaaaagaat
    18601 acagattggg aatgccaggc ctgccagaat cgcgtggatg atgtacagga tcggcgcaac
    18661 gaacatgaag gagaactcga tcggctcggt gataccggtc aggaacgagg tcagcgccgc
    18721 ggagatcata ataccgccca ctttcgcgcg gttttctggt ttagcagagt gccagatagc
    18781 aattgcggca gctggcagac cgtacatttt gaacaggaag ccaccagaca gtttacccgc
    18841 agtcgggtca cccgccatat aacgcggaat gtcgccgtgg aaaacctgac ctgctgcgtt
    18901 ggtgtattca ccaatttgca tctggaaagg tacgttccag atgtggtgca gaccaaacgg
    18961 taccaggcaa cgttcgatga aaccgtaaat gccaaacgca actaccgggt tctggtaagc
    19021 agcccactga gagaaggtct ggattgcaga accaatcggc ggccaaatga aggacagcac
    19081 aacgccagta aagatggcag ccaggccaga aatgatcggc acaaagcgtt taccggcaaa
    19141 gaagccaaga tactcaggca gcttaatacg gtagaaacgg ttaaacatgt acgctgcgat
    19201 cgcaccggag ataatccccc cgagtacgcc agtatccgcc aggtgtttag aggcaatttc
    19261 ttcagcaggt aaatgcagta ccagtggcgc aaccacggcc atggttttaa ccatgatgcc
    19321 ataggcaaca actgcggcca gcgcggatac gccatcgtta ttggtaaagc cgagggcgac
    19381 accgatcgca aaaatcagtg gcatgtttgc aaagacggaa ccgcctgctt ctgccataac
    19441 atgcgataca acggcgggca gccagctgaa attcgcggaa ccgacgccca gcagaatacc
    19501 tgcgataggc agtacggata ccggcagcat cagcgattta ccgacctttt gcaggttagc
    19561 aaatgcattc ttaaacataa ttgagagtgc tcctgagtat gggtgctttt tttacgttct
    19621 cacgcgtggc aaggggggag agcctcgccg tgtacagggc atctaagcgc cctttattta
    19681 ttacacagag taaaataatt cagtgccgat ttgtttgacg gctatcacgt ttcaacttca
    19741 gatcattgga gaagttgcac attttgacaa aacatatgtc acaatatttc aaacgtggtt
    19801 attcaccgcc caattttcgg cggtgaactt tactcgtttt agccattaat tacgagcttt
    19861 aaaaaaactc attcaacgga tggattgaag gcgggaagcg tcgatgtgaa acagacgggc
    19921 gaagttatcg gtggttacct gcgccagttc ttcaacggca acacctttca acacagccat
    19981 gtattctgca acgtcacgaa ccatcgcagg ttgattctct tttcctcgat gcggtaccgg
    20041 cgcaaggtaa ggtgagtcag tttccaccag taaccgatcc agggggacat aacgcgcagc
    20101 atcgcgcagt tgctccgcat tacggaaggt cacaatgccg gaaaaggaga tgtaaaatcc
    20161 gagatccagt aatttacccg ccgtttctct atcctctgta aaacagtgta gtacgccacc
    20221 gcaatccgtc actttttctt cgcgcagaat tgccagcgta tcggcgcggg cgtcacgggt
    20281 gtggacgata accggcttgt tcagttcacg accaatctgg atatgatgga tgaaggactc
    20341 ttgctgacgt actttagttt ccggcgtgta ataataatcc agcccggttt cacccagcgc
    20401 aacaacgccc tcttctgccg ccagacggcg taaatcttct acatcgtagg gatcattctg
    20461 gttgagtgga tgcacgccac aagaaaatac gacgttgtca cgttcgccta ccagatcccg
    20521 catgtgtaaa taacccggta atgttgtggc gactgccaga caaaatttca catcgcgtgc
    20581 ggcggctttc gccagaacgt catccacgtc cttatgcaaa gattcataat ccagaccatc
    20641 gagatggcag tgtgagtcga ctaaaaacat gatgtctctc ttaaagatga ggaaccggta
    20701 gcacaacgcc cggttgcagg taatgctcaa tacgcagtaa aagatcggtg atgagaagct
    20761 cgcggttgat gcctgtgaca gacattaact gttcacgaat gtggcaaaca tcccccagta
    20821 tagcctgcag gcgcgaggga gatagatggt ttgccagttc gacgaccagg cccggcacat
    20881 caacattggt cacctgcgca gcgccatgat ggcgttttag cgcatccatc agcaacgttg
    20941 ccagccagtg taaacgcgcc ggagcttgtt catgattaag ggccgccagc agcgaatacc
    21001 agtcgcccga tggcacgcta tatgccaacg cctgacacaa tgtttcacga gcctgccagt
    21061 tatctccctg aaacaacgcc agtgccgcgc caggcgaacc ggcgcttaag cgcaatgcgg
    21121 caagtaatgc atcctgtgac attgtcactt cgcgtgaaag ccaggtcacg gcgtactgtt
    21181 ccggcggcgg cgcaaggtaa tgtaaccgac aacgactacg taatgttgcc agtaaacgtt
    21241 caggctcgcg ggtagccagg aaaaaccagg tttctgctgg tggctcttca agcgttttca
    21301 gcaatgcgtt agccgcggcg tcggtcagta aggcagcatc ggttacccag acaactttcg
    21361 caccacctaa acgtgcgtgc tcatttaact tttcggtgac ttcacgtacc gcatcaatgc
    21421 ccagcgcatt tttccctttc tcgggagcca gggtgtaata atcgggatgc gtaccagcct
    21481 gcatcaactg acatccacga cagtgaccgc aacttttgtg gccctgcggt tgttggcaga
    21541 gtaaataacg gctcagggcg tagattaaag catcctcgcc catacccggt aacgcctgaa
    21601 tgagtagcgc atggtgacct cttccggcct gatagctggc taccagtttt tcgaaatcag
    21661 gtcgtaacca tggataccat ctcatgcgtc caactccttc acccaattgg tcacggtagt
    21721 gcggattgca tccatcacgg actccagcgg ctgggtggca tcaatggtat gaatgctttt
    21781 atcttgtgct gccagttcca gatagcgggc gcgggtgcga ttaaagaaat cgaaagactc
    21841 ttgctcaata cgatccagct cgccgcgagc acgcgcacgt tttaagccaa cttccggggt
    21901 aacatcgaga tagagcgtta agtcggggcg aaaatccccg agaacagcat cacgcaatgt
    21961 tgccagcata tgttggtcaa taccacgtcc gccgccctga tacgcctgag tggagagatc
    22021 gtggcgatcg ccaatcaccc aagtgccgtt agccagcgct ggtttgatga ccgtttctac
    22081 cagttgaacg cgcgcggcat aaaacatcag aacttcggct ttatcggtaa tgacttcatc
    22141 gcctaccgat ttgatatcca gcaccaggct tcttaacttt tcggcaagtt gcgtaccgcc
    22201 aggttcccga gtgaaaacca tgtcgcggat acccagttgc tcgagcgtct caaccaccac
    22261 gttacgcgcg gtagttttgc ctgcgccttc cagcccctca atgacgatat acttactgcg
    22321 catttttttc cttaagcact ttcagataat cctgcacaga cttgttatga ctggcaagat
    22381 tggtattaaa cgtgtgacca cctttaccat cggccacaaa atagagatac ggcgtttttg
    22441 ccggatgcgc agcagccttc agcgaatccg cccccggcgt agctattgca cctggcggca
    22501 aaccggtaat ggtataggtg ttatacgctg tcggcgtttc caggtctgca cgagaaagtt
    22561 tgccattata acgctctccc atcccgtaaa tcacggtcgg gtcggtctgc aggcgcatac
    22621 caatgcgtaa ccggttgata aataccgagg caacctgatc gcgttcactg gcaacggcgg
    22681 tttctttttc gataattgat gccatcgtca ccagctggtt tttatcttta tagggcagac
    22741 cgtccgcacg cccttcccag gcgctatcga ccgctttcac cattttcttg tgcgctcgct
    22801 tgagtaacgc gacatcggtg gtattggcgg tatacatcca ggtgtctggc cagaaccaac
    22861 cttcaatcca ctctgggttt tccagttcaa gtgcctgcgc tacggtggcg tacttatcgt
    22921 cgctcagcgt atgcttgata tacggggcct cacgcaattg cttgaggtaa tcgctcagac
    22981 gcatcccttc taccagtcgc agagggaact gtgcttcttt accgctttct agcaatttca
    23041 gcatctcgcg cacggtcatc tgcggtgtaa agcggtaagt cccggcttta aagtgagaaa
    23101 gatccggttc gatacgcagc agccattgga aaacccgtgg gcgattgatg atcttatcgg
    23161 cataaagctg ttcaccgagc gccagacgtc cggtcccagc cttcagggta aatatcgtct
    23221 cttctttgat aagcaatttg ctgtcggcaa gatggcgaac cttccagacg cccacaccag
    23281 cggcgatacc cagtaccacc agcaataaca agataatcaa taacactttt ttcatgacta
    23341 attcgggcgc tcacaaagtg gggctaaata ttcatataac gttgctgacg aaaaggagac
    23401 atcgccacag gcacgtacgg gcatcactgg cattaacgca ttacaaataa ccatctcatc
    23461 tgcctgcaac gcctcctcca gcgaggcttg cacttcgaca agctgataag aggattgtgc
    23521 cagtaaacgg atacagaatt gtcgcataat gccgttaaca cctgcctgat ccagtcgcgg
    23581 cgtataaact acgttgccct tccgccagaa caaattagcc gcacagcatt ccgtaaccca
    23641 cccttcgctg tcaaggacca gcgcctcatc agcgtttgtc tgctcaagat gagagcggat
    23701 caatacttgc tcaagccgat taagatgttt aatacctgcc agatgaggat tgcgccccag
    23761 ccgcaccggg cttagcgcca acgtcatccc ctcgttacgc aaacggtcgt aatgtgcggg
    23821 ataaaccgta acggagagaa tccgcgttgc cggtccgctg ttcaatgtgc tgtatcctcg
    23881 cccgccacta ccgcgactga tcaccacttt cagcacacca ttttgctgtt ctgctgccag
    23941 cgttttcatc tctagttcaa gctgaggcca gaagtcacag gaaatcatca accgctgaca
    24001 agcatcctgt agtcgctgga tatgcgccga taacaaactg actttaccgt cgataactct
    24061 ggcggtggta aaacaaccat caccaaactg cgttgcccga tcgcttactg ccagcgattc
    24121 ctgcttatga ccgttaatta agaacatacc ggctccttat cagagaattg ttagtgtggc
    24181 agcatgttca ctacggaaca agtcggaata aaagctaaga aaaaaggccc gcaagcggac
    24241 cttttataag ggtgggaaat gacaacttag atctttttaa agatcaaaga accattagtg
    24301 ccaccgaagc cgaaggagtt acacagcgtg tattccattc cgcttacctg gcgcgcttcg
    24361 tgcggtacga aatccagatc gcaaccttca tccgggttat ccaggttgat ggttggcgga
    24421 acagcctgat cgcgcagcgc caggatggag tagatagatt ctactgcacc cgccgcacct
    24481 aacaggtgac cggtcataga tttcgtggag cttaccaaca cacggcttgc agcttcaccg
    24541 aagatggttt tcaccgcctg cgcttcagct ttatcgccag ccggcgtaga agtaccgtgc
    24601 gcgttaacgt agccaatctg actcgcttca atgcctgcat cacgcagagc atttgccatc
    24661 gccagagctg cgcctgcgcc attttctggc ggtgacgtca tatgataagc atcgctgctc
    24721 ataccaaagc cgacgagttc agcgtaaatt ttcgcaccgc gttttttcgc gtgttcgtac
    24781 tcttcaagta ccagcatacc ggcaccatcg cccagtacga aaccatcacg ctctttatcc
    24841 cacgggcggc tcgccgcttg cgggttatca ttgcgggtag ataatgcacg tgccgcgcca
    24901 aaaccaccaa cgcccagcgg cgtactggct ttctctgcgc cacctgcaac catcacgtca
    24961 gcatcgccat acgcgataat acgcgcagca tggccaatgt tgtgcacgcc ggaagtacag
    25021 gcggtcgcga tagagatgct tgggccacgc aggccataca tgatagtcag atgacctgcc
    25081 accatgttca caatcgttga cggaacgaag aatgggctga tcttacgtgg accaccgttc
    25141 atcagagatg tgtggttttc ttcgatcagt ccgaggccgc caatcccgga gccaattgcg
    25201 gcaccaatgc gggttgcgtt ctcttccgtt atttcaaggc cagaatcctg catggcctga
    25261 acgccagcga caattccata ttgaatgaag gcatccatct tgcgctgttc tttgcgcgag
    25321 ataatgtcct cacagttaaa atcctttact aagccagcaa atttcgttgc ataggcgcta
    25381 gtatcgaaat ggtcgattag gctgatgcca ctctgaccgg caagcagagc tttccaggta
    25441 gactctacgg tattgccgac aggagacaac atgcccagtc cggtcacaac tacacgacgc
    25501 ttagacacgt ttgtcctcca gggagggaaa aaatgattct agtgggacaa aaagataaaa
    25561 ctcaggcggt cgaacgaccg cctggagatg ttcacttacg cctggtggcc gttgatgtaa
    25621 tcaatggcag cctgaacggt ggtgattttc tcagcttctt cgtccggaat ctcagtatca
    25681 aactcttctt ccagagccat taccagctca acggtgtcaa gagaatccgc gcccaggtct
    25741 tcaacgaaag aagcattgtt ggtaacttct tcctgcttaa cgcccagctg ttcgccgata
    25801 attttcttaa cgcgttcttc gatagtgctc atactcttaa atttcctatc aaaactcgct
    25861 ttcgcgatgg ttttcgtagt gtataaaatg ttgaaaaatt tgcaactaaa tcccggcagg
    25921 tcttaccacg attttacgtt attttgaggc cttacccccc aataacgcaa atcattttgc
    25981 actaattatt actcataacc acgcagactg cgcgcaacat gagcattttg tgcaaatcgc
    26041 ggtcagacca tgtacatccc gccgttcaca tgcaaagttt cacccgtgat gtaagctgct
    26101 tcgtcggatg ccaggaatgc aaccgcgttg gcgatttcct gtgcgccgcc gaggcgaccc
    26161 gcaggaacct gcgccaggat acccgcacgc tggtcatcgc tcagcgcacg tgtcatgtcc
    26221 gtttcaataa agcccggagc aacaacgttt acagtaatac cgcgtgacgc aacttcgcgc
    26281 gccagtgatt tactgaagcc gatcaagccc gctttcgccg cagcgtagtt ggcctgaccg
    26341 ccatttccca tggtaccaac cacagaaccg atagtgataa tacgaccatg acgctttttc
    26401 atcatagcgc gcattaccgc ttttgacaga cggaaaacag atgaaaggtt ggtttcgata
    26461 atatcgttcc actcttcatc tttcattcgc attaacaggt tatcacgagt gataccggca
    26521 ttattgacca ggatatccac ttcaccaaat tctgcgcgaa ttttttccag aacagattcg
    26581 atagatgccg ggtcggtcac attcaacatc agacctttgc cgttggcacc taaataatca
    26641 ctgatcgcct gagcgccatt ttcactggtc gctgtgccaa taactttcgc gccacgggct
    26701 gcgagcgttt cagcaattgc gcggccaatt ccgcggcttg caccggttac cagtgcgatt
    26761 tttccttcaa aattcatgat tttcctcttt taaagctcga gcgccgctgc catcgctgaa
    26821 ggttcgttca gcgccgaggc ggtcagggtg tcgacaatgc gtttcgtcag gccagtaagc
    26881 actttgcccg ggccgacttc atagagatgt tctacgcctt gcgctgccat gtattcaaca
    26941 gacttcgtcc actgaaccgg gttatacaac tgacgtacca gtgcgtcacg gatggcatca
    27001 ccattggttt cgcatttcac atcaacgtta ttcacaacag gaactgttgg tgcgttaaag
    27061 gtgattttcg ctaattctac tgccagtttg tcggctgctg gtttcatcag cgcacagtga
    27121 gacggtacgc tcactggtaa cggtagagcg cgttttgcgc ccgcagcttt acaggcagcg
    27181 ccagcacgct caactgcttc tttatgaccg gcaataacca cctgtcccgg agagttaaag
    27241 tttaccggag aaacgacctg accttctgca gcttcttcac acgctttcgc aatagacgca
    27301 tcatccagac cgatgattgc cgccatagcg cccgttcctt ccggtacggc ttcttgcatg
    27361 aacttgccgc gcatctcaac cagacgcacc gcatcagcga aatcaatcac accagcgcaa
    27421 accagcgcgg agtattcccc caggctgtga ccagccatca ttgccggtgc tttaccgccc
    27481 tgctgctgcc atacgcgata cagcgcaaca gatgcagtca acagcgctgg ctgggtttgc
    27541 caggttttat tcagttcttc agctggcccc tgctgggtca gcgcccacag gtcgtagccc
    27601 agcgccgcag aagcttcagc aaacgtttct tcgacaattg gatagctcgc cgccatatca
    27661 gccagcattc caacggtttg agaaccctgt ccagggaaca caaatgcaaa ttgcgtcatg
    27721 ttttaatcct tatcctagaa acgaaccagc gcggagcccc aggtgaatcc accgccaaag
    27781 gcttcaagca gaaccaactg ccccggctta atgcgcccgt cgcgtacagc ttcatccaga
    27841 gcgcacggga cagaggccgc agaggtatta ccgtggcgat ccagcgtcac cacgacattg
    27901 tccatcgaca taccgagttt tttcgccgtt gcactgataa tacgcaggtt agcctgatgc
    27961 ggaaccagcc agtccagttg agaacggtca agattattcg ccgccagcgt ctcatcaacg
    28021 atgtgcgcca gttctgttac cgcaaccttg aagacttcgt tgcccgccat cgtcagatga
    28081 attgaattct ctggattcac acggtcagca ttaggcagcg tcagcaactc accatagcta
    28141 ccgtcggcat gcagatgggt ggagatgatt cccggctctt cagaggcagc cagcaccgca
    28201 gcgcccgcgc catcgccaaa aataataata gtcccacgat ctgttggatc gcaggtgcgc
    28261 gccagtacat cggaaccgac gaccagagca tacttcaccg ccccagattt cacgtattga
    28321 tcggctacgc ttaatgcata ggtgaaacct gcgcaggctg ctgcaacgtc aaatgccggg
    28381 caacctttaa tgcccaacat gctttgaatc tgacaagctg cgctcgggaa agcgtgcgta
    28441 gcagaagtcg ttgccacaac gatcaggcca atctggtctt tctcaatgcc cgccatctca
    28501 attgcgcgtg tcgccgcttc aaagcccatg gttgaaacgg tttcgtttgg cgcggcaatg
    28561 tggcgttcgc ggataccggt acgagtgaca atccactcgt cagaggtgtc caccattttt
    28621 tccaaatcgg cgtttgtccg cacttgttcg ggcagatagc tgccagtacc aataatcttc
    28681 gtatacatgt acgctcagtc acttttcggt tatataccgt cacttgcaaa ctgcgagttc
    28741 gctggcagcg tcctgctacc gcagagttcc gcttttgcca ccgtccagca gctcaaaacc
    28801 agctgggtat acagattcca ggcgagcggc aattcgctga ggaacttgtc gctgcaccgc
    28861 ctgcactgcc tgttcaatcg cgaccgcaaa agctcgctga ttggctgcac catgactttt
    28921 tatcaccgtg ccgcgcaatc ctaacagaca ggcgccgtta tactggtcgg ggttgaggtg
    28981 actgaatcgc ctcgtcaggc tcttttgtag ccaacgcttt aataacagta gccaccacga
    29041 ccgttttttc ccttcaccct gagatttcag cagagaaagg aacatcctga caacaccttc
    29101 catcgtcttt aatgtgacat ttcctgtaaa gccatcacaa accagcacat ctgtcttgcc
    29161 agttaacaac tcattggctt caagatagcc gatataattg atagaaggga ttgttttaag
    29221 caccgctgag gcatcccgaa tactgtcgag accctttact tcttcttcac caatattgag
    29281 caacgccacg cgaggattag gaatttccac cacctcttca gccagaactg agcccataat
    29341 ggcaaattgc accaacattg tactgtcaca atcgacgttg gcccctaaat cgaggaccac
    29401 cgttttgccc ttttgctgat gcggtaatac cgtcaccagc gccggacgct caatcccctc
    29461 caggggcttg agtaataatt ttgccagccc catcagcgcc ccggtattac cggcactgac
    29521 acaggcttgc gctcgaccct ctttcaccag ctccagggcc acgcgcattg aactcccacg
    29581 actggcgcgg atagcttgcg aaggccgggc atcactggcg ataactgact gcgcaggaat
    29641 aatctgcaga cgcgaacg
//
LOCUS       DAMZFT010000053        29493 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.053, whole genome shotgun sequence.
ACCESSION   DAMZFT010000053 DAMZFT010000000
VERSION     DAMZFT010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 29493)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29493)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29493
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.053"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(<1..367)
                     /locus_tag="PN977_002441"
     CDS             complement(<1..367)
                     /locus_tag="PN977_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegQ"
                     /protein_id="HDI6042464.1"
                     /translation="MKKQTQLLSALALSVGLTLSASFQAVASIPGQVADQAPLPSLAP
                     MLEKVLPAVVSVRVEGTASQGQKIPEEFKKFFGDDLPDQPAQPFEGLGSGVIINASKG
                     YVLTNNHVINQAQKISIQLN"
     gene            complement(521..919)
                     /locus_tag="PN977_002442"
     CDS             complement(521..919)
                     /locus_tag="PN977_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1043 family protein"
                     /protein_id="HDI6042465.1"
                     /translation="MTWEYALIGLVVGIIIGAVAMRFGNRKLRQQQALQYELEKNKAE
                     LDEYREELVSHFARSAELLDTMAHDYRQLYQHMAKSSSSLLPELSAEANPFRNRLAES
                     EASNDQAPVQMPRDYSEGASGLLRTGAKRD"
     gene            1113..2240
                     /gene="zapE"
                     /locus_tag="PN977_002443"
     CDS             1113..2240
                     /gene="zapE"
                     /locus_tag="PN977_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapE"
                     /protein_id="HDI6042466.1"
                     /translation="MQSVTPTSQYLKALNEGSHQPDDVQKEAVSRLEIIYQELINSTP
                     PAPRTSGLMARVGKLWSKREDTKHTPVRGLYMWGGVGRGKTWLMDLFYQSLPGERKQR
                     LHFHRFMLRVHEELTELQGQSDPLEIIADRFKAETDVLCFDEFFVSDITDAMLLGGLM
                     KALFARGITLVATSNIPPDELYRNGLQRARFLPAIDAIKQHCDVMNVDAGVDYRLRTL
                     TQAHLWLSPLNDETRAQMDKLWLALAGAKRENSPTLEINHRPLATMGVENQTLAVSFT
                     TLCVDARSQHDYIALSRLFHTVMLFDVPVMTRLMESEARRFIALVDEFYERHVKLVVS
                     AEVPLYDIYQGERLKFEFQRCLSRLQEMQSEEYLKREHLAG"
     gene            2459..2887
                     /gene="rplM"
                     /locus_tag="PN977_002444"
     CDS             2459..2887
                     /gene="rplM"
                     /locus_tag="PN977_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HDI6042467.1"
                     /translation="MKTFTAKPETVKRDWYVVDATGKTLGRLATELARRLRGKHKAEY
                     TPHVDTGDYIIVLNADKVAVTGNKRTDKVYYHHTGHIGGIKQATFEEMIARRPERVIE
                     IAVKGMLPKGPLGRAMFRKLKVYAGNEHNHAAQQPQVLDI"
     gene            2903..3295
                     /gene="rpsI"
                     /locus_tag="PN977_002445"
     CDS             2903..3295
                     /gene="rpsI"
                     /locus_tag="PN977_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000829824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HDI6042468.1"
                     /translation="MAENQYYGTGRRKSSAARVFIKPGNGKIVINQRSLEQYFGRETA
                     RMVVRQPLELVDMVEKLDLYITVKGGGISGQAGAIRHGITRALMEYDESLRSELRKAG
                     FVTRDARQVERKKVGLRKARRRPQFSKR"
     gene            3689..4327
                     /gene="sspA"
                     /locus_tag="PN977_002446"
     CDS             3689..4327
                     /gene="sspA"
                     /locus_tag="PN977_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stringent starvation protein A"
                     /protein_id="HDI6042469.1"
                     /translation="MAVAANKRSVMTLFSGPTDIYSHQVRIVLAEKGVSFEIEHVEKD
                     NPPQDLIDLNPNQSVPTLVDRELTLWESRIIMEYLDERFPHPPLMPVYPVARGESRLY
                     MHRIEKDWYTLMNTIINGSASEADAARKQLREELLAIAPVFGQKPYFLSDEFSLVDCY
                     LAPLLWRLPQLGIEFSGPGAKELKGYMTRVFERDSFLASLTEAEREMRLGRS"
     gene            4333..4830
                     /gene="sspB"
                     /locus_tag="PN977_002447"
     CDS             4333..4830
                     /gene="sspB"
                     /locus_tag="PN977_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor"
                     /protein_id="HDI6042470.1"
                     /translation="MDLSQLTPRRPYLLRAFYEWLLDNQLTPHLVVDVTLPGVQVPME
                     YARDGQIVLNIAPRAVGNLELSNDEVRFNARFGGIPRQVSVPLAAVLAIYARENGAGT
                     MFEPEAAYDEDASIMNDEEASADNETVMSVIDGDKPDHDDDTHPDDEPPQPPRGGRPA
                     LRVVK"
     gene            complement(4874..6241)
                     /gene="dcuC"
                     /locus_tag="PN977_002448"
     CDS             complement(4874..6241)
                     /gene="dcuC"
                     /locus_tag="PN977_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HDI6042471.1"
                     /translation="MFGIIISVIVLITMGYLILKNYKPQVVLAAAGIFLMMCGVWLGF
                     GGVLDPAKSSGYLIVDIYNEILRMLSNRIAGLGLSIMAVGGYARYMERIGASRAMVSL
                     LSRPLKLIRSPYIILSATYVIGQIMAQFITSASGLGMLLMVTLFPTLVSLGVSRLSAV
                     AVIATTMSIEWGILETNSIFAAQVAGMKIATYFFHYQLPVASCVIISVAISHFFVQRA
                     FDKKDKHINHEQAEQKALDNVPPLYYAILPVMPLILMLGSLFLAHVGLMQSELHLVVV
                     MLLSLTVTMFVEFFRKHNLRETMDDVQAFFDGMGTQFANVVTLVVAGEIFAKGLTTIG
                     TVDAVIRGAEHSGLGGIGVMIIMALVIAICAIVMGSGNAPFMSFASLIPNIAAGLHVP
                     AVVMIMPMHFATTLARAVSPITAVVVVTSGIAGVSPFAVVKRTAIPMAVGFVVNMIAT
                     ITLFY"
     gene            6621..7412
                     /gene="nanR"
                     /locus_tag="PN977_002449"
     CDS             6621..7412
                     /gene="nanR"
                     /locus_tag="PN977_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312126.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NanR"
                     /protein_id="HDI6042472.1"
                     /translation="MGLMNAFDSQTEDSSPVIGRNLRSRPLARKKLSEMVEEELEQMI
                     RRREFGEGEQLPSERELMAFFNVGRPSVREALAALKRKGLVQINNGERARVSRPSADT
                     IIGELSGMAKDFLSHPGGIAHFEQLRLFFESSLVRYAAEHATDEQIDLLAKALEINSQ
                     SLDNNAAFIRSDVDFHRVLAEIPGNPIFMAIHVALLDWLIAARPTVADQALHEHNNVS
                     YQQHIAIVDAIRRHDPDEADRALQSHLNSVSATWHAFGQTTNKKK"
     gene            7534..8427
                     /gene="nanA"
                     /locus_tag="PN977_002450"
     CDS             7534..8427
                     /gene="nanA"
                     /locus_tag="PN977_002450"
                     /EC_number="4.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HDI6042473.1"
                     /translation="MATNLRGVMAALLTPFDQQQALDKASLRRLVQFNIQQGIDGLYV
                     GGSTGEAFVQSLSEREQVLEIVAEEAKGKIKLIAHVGCVSTAESQQLAASAKRYGFDA
                     VSAVTPFYYPFSFEEHCDHYRAIIDSADGLPMVVYNIPALSGVKLSLDQINTLVTLPG
                     VGALKQTSGDLYQMEQIRREHPDLVLYNGYDEIFASGLLAGADGGIGSTYNIMGWRYQ
                     GIVKALKEGDIQTAQKLQTECNKVIDLLIKTGVFRGLKTVLHYMDVVSVPLCRKPFGP
                     VDEKYLPELKALAQQLMQERG"
     gene            8536..10026
                     /gene="nanT"
                     /locus_tag="PN977_002451"
     CDS             8536..10026
                     /gene="nanT"
                     /locus_tag="PN977_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialic acid transporter NanT"
                     /protein_id="HDI6042474.1"
                     /translation="MSTTTQNIPWYRHLNRAQWRAFSAAWLGYLLDGFDFVLIALVLT
                     EVQGEFGLTTVQAASLISAAFISRWFGGLMLGAMGDRYGRRLAMVTSIVLFSAGTLAC
                     GFAPGYITMFIARLVIGMGMAGEYGSSATYVIESWPKHLRNKASGFLISGFSVGAVVA
                     AQVYSLVVPVWGWRALFFIGILPIIFALWLRKNIPEAEDWKEKHGGKAPVRTMVDILY
                     RGEHRIANIVMTLAAATALWFCFAGNLQNAAIVAVLGLLCAAIFISFMVQSTGKRWPT
                     GVMLMVVVLFAFLYSWPIQALLPTYLKTDLAYDPHTVANVLFFSGFGAAVGCCVGGFL
                     GDWLGTRKAYVCSLLASQLLIIPVFAIGGANVWVLGLLLFFQQMLGQGIAGILPKLIG
                     GYFDTDQRAAGLGFTYNVGALGGALAPIIGALIAQRLDLGTALASLSFSLTFVVILLI
                     GLDMPSRVQRWLRPEALRTHDAIDGKPFSGAVPFGSAKNDLVKTKS"
     gene            10074..10763
                     /locus_tag="PN977_002452"
     CDS             10074..10763
                     /locus_tag="PN977_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HDI6042475.1"
                     /translation="MSLLAQLDQKIAANGGLIVSCQPVPDSPLDKPEIVAAMALAAEQ
                     AGAVAIRIEGVANLQATRAVVSVPIIGIVKRDLEDSPVRITAYIEDVDALAQAGADII
                     AIDGTDRPRPVPVETLLARIHHHGLLAMTDCSTPEDGLACQKLGAEIIGTTLSGYTTP
                     ETPEEPDLALVKTLSDAGCRVIAEGRYNTPAQAADAMRHGAWAVTVGSAITRLEHICQ
                     WYNTAMKKAVL"
     gene            10760..11635
                     /gene="nanK"
                     /locus_tag="PN977_002453"
     CDS             10760..11635
                     /gene="nanK"
                     /locus_tag="PN977_002453"
                     /EC_number="2.7.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312122.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine kinase"
                     /protein_id="HDI6042476.1"
                     /translation="MTTLAIDIGGTKLAAALIGADGQIRDRRELPTPASQTPEALRDA
                     LSALVSPLQVHAQRVAIASTGIIRDGSLLALNPHNLGGLLHFPLVKTLEQLTDLPTIA
                     INDAQAAAWAEYQALEGDITEMVFITVSTGVGGGVVSGGKLLTGPGGLAGHIGHTLAD
                     PHGPVCGCGRTGCVEAIASGRGIAAAAQGELAGANAKTIFTHAGQGDEQAQQLIHRSA
                     RTLARLIADIKATTDCQCVVVGGSVGLAEGYLALVETYLAQEPAAFHVDLLAAHYRHD
                     AGLLGAALLAQGEKL"
     gene            11632..12096
                     /locus_tag="PN977_002454"
     CDS             11632..12096
                     /locus_tag="PN977_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HDI6042477.1"
                     /translation="MMMGEVQSLPSAGLHPALQDALTLALAARPQEKAPGRYELQGDN
                     IFMNVMTFNTQSPVEKKAELHEQYIDIQLLLNGEERILFGMAGTARQCEEFHHEDDYQ
                     LCSAIENEQTIILKPGMFAVFMPGEPHKPGCVVGEPDEIKKVVVKVKADLMA"
     gene            12225..12797
                     /locus_tag="PN977_002455"
     CDS             12225..12797
                     /locus_tag="PN977_002455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6042478.1"
                     /translation="MALLSLSFVRLNFGRMGNRLTEKNNQMISFLSDEFEEFPQGDFL
                     NHLYRFSVDHHHFIVIFSPIDISDAEYHQYRSEEVGFEIPTNCFDVKFDRQENFDSGN
                     FYMPPAATECSRRRRFTDELAEALATIIEKHYIIYHARAYLAIAENDKLKRYYDRILH
                     NAPANVAYRVIKDVGEEERGYAIQTECFRT"
     gene            12766..12912
                     /locus_tag="PN977_002456"
     CDS             12766..12912
                     /locus_tag="PN977_002456"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6042479.1"
                     /translation="MPYKPNVSELKKMTVAERKKEAMRQLKASFERASIEGTLHKRSA
                     KSVA"
     gene            complement(13134..14303)
                     /locus_tag="PN977_002457"
     CDS             complement(13134..14303)
                     /locus_tag="PN977_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000097505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HDI6042480.1"
                     /translation="MSSNEMERLRRWRLILGEDADNACNVALDARESAIDAALAALYQ
                     PEGKHGLRGGTGGSSPKVARWLGDIRQYFPSSVVQVMQKDAFERLNLHSMLLEPEMLA
                     NVQPDVHLVSTLMSLNGVIPAKTKETARLVVRKVVEQLMKQLEEPMRSAVSGALNRAV
                     RNRRPRHAEIDWQRTIRANLRHWQEEYKSIVPETLIGYGRKSQRTQKEIILCIDQSGS
                     MASSVVYSSIFGAVMASLPAVKTHLVVFDTAVVDMTEKLDDPVELLFGVQLGGGTDIN
                     RAVGYCQSLIRDPRNTILVLISDLYEGGVERNLLQRASELIQSGVQVVTLLALSDEGA
                     PFYDRSLAGKLAAMGIPSFACTPDLFPGMMAAAIRKEDVNLWAAQNGVVTARETA"
     gene            complement(14293..16542)
                     /locus_tag="PN977_002458"
     CDS             complement(14293..16542)
                     /locus_tag="PN977_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000139765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6042481.1"
                     /translation="MTEQTVHLFGIRHHGPGCARSLRKALETLQPDCLLVEGPPDGES
                     ILPFMQHEQMQPPVALLIYAPDDSHHAAFYPFAAFSPEWQALRYGFEQNIAVRFMDLP
                     ISHQFALDDEKESDDDDAVEASPDGDPLDWLGRAAGYTDGESWWSHRVEEREDDLSLF
                     EAIREAMIALRQATPEARNSARDQLREAYMRKTLRQAKKEGFSRIAVVCGAWHVPALE
                     NLPPAKNDNELLKNLPKCKVAAAWTPWSYEALSRASGYGAGVVSPEWYDHLWRYQGAS
                     HRDIGWLSRAARLFREADLDCSSAHIIEAARLAQTLAIMRHHPQPGLDELCEALQTVV
                     CMGESAPMQMIRQKLIVGDALGSVPDDTPVVPLQRDITQQQKTLRLKAEASEKVLDLD
                     LRKPGDLARSHLLHRLTLLDISWGRLAGQGNNSKGTFHEVWSLRWEPALAINIITASR
                     WGNSIEQASSRYAIVRAQYASTLPELAKLIQQVLLADLQTAIAPIANTLESLAATQGN
                     IEQLLEALAPLVAIVRYGNVRQTDSGMVMQVLMSLAPRAAIALPGACSALNDDSAASM
                     REKVIDAHAALRLLDNEDLLAGWLQALMVLAEGSTAHALLRGTATRLLFDLQTLTTER
                     ISTLMNLALSPANPPAESAAWAEGFLNDSAMVLLHNSELWQLIDAWLSGLNDNHFTRI
                     LPMLRRTFGRFSSPERRQLGERAAQGERVAQQEETSGIWDEQRAALMLPLLRRILALP
                     PHERENHVE"
     gene            complement(16560..17654)
                     /locus_tag="PN977_002459"
     CDS             complement(16560..17654)
                     /locus_tag="PN977_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000994483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDI6042482.1"
                     /translation="MTSTATAAVLRRHAEQQFAEELDELQKNDTHPRPENWAISPWSV
                     ATYLLGGQLENGFEVTPKYIGNRRIIETAVATLATDRALLLYGVPGTAKSWVSEHLAA
                     AISGDSTLIIQGTAGTGEEQMRYGWNYAELLSKGPSRSALVESPLMRAMARGQIARVE
                     ELTRIPADVQDTLITILSEKTLPIPELNEEVQATRGFNVIATANNRDKGVNELSSALK
                     RRFNTVILPVPASEEEEINIVTRRVAELGQALALPAEPPALKEIQRVVQIFRELRNGL
                     TVDGKTKIKSPTSTLSTAEAISVINNGMALAGHFGDGVLHPRDIAASLIGAVVKDPVQ
                     DDVVWREYLETIVKERSDWNDLYRACREIV"
     gene            complement(17651..19255)
                     /locus_tag="PN977_002460"
     CDS             complement(17651..19255)
                     /locus_tag="PN977_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001007101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6042483.1"
                     /translation="MNEWQNLTRTALLGTDKKAFTPSASQSEIGLLLSELANNTSAEQ
                     QLLRSAGVLALCHLAGWVPQAIDPAPLPEIDKENAEPINNPAFAGLLHLLLSEGPPRL
                     LWSALDLLVRRQLLPPPLLLPALLNYGAQKPSLRGILSNVLGARGCWLAQINADWGYV
                     LASTDAPLDEEMWLHGTSEQRLQYLTQMRIRAPEQGRDRLAQEMSSLDARERAQLLAV
                     LEVGISQHDEAFLEQTLSDRSKEVRQTAARLLCCLPESAWISRMKSRLAPLLSSSPAP
                     ETLLERLKGLSGKEKMLRVALDAPEAFLPEWKADALEETKPKGEKLGQRAWWLYQIVA
                     AVPLDWWQTQLQATPVELLRWAGKTDWQEALLRAWYHAVLREKNPQWAQAFLALLPVG
                     ISIHSPAGVKINAFELLQCLPVEEHEPVLTLLFSVMGGEHLQRYIALLPLDASLFSLR
                     LSQQMVTELHRWVKHDSARFDYALRHVMSDFACLLAPEVLNDVIDKWPHDAQQTPYCE
                     AAFTALSATLAHRIQLHSFFVGETTL"
     gene            complement(19252..20574)
                     /locus_tag="PN977_002461"
     CDS             complement(19252..20574)
                     /locus_tag="PN977_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HDI6042484.1"
                     /translation="MELTVESVQALAPDDASVKAARGLVAPAKWPTLGYSEAAFWGEC
                     KGSGSRPYQVRIDRQDLACKCSCPSRKFPCKHSLALLFLQVQHTASFTAGEPPEWVSE
                     WLTSRQQRAVRKEEKKEQAEAKAADPQAAAKREAARNQKMTAGLDFLEQWMHDLIRHG
                     LAQISAQQLPFAGIAARMVDAQLPGIAARLNNLTTHFTTAEVWPSSLCKELGQLQLII
                     DAWRQQQMLSPAQLSDLHAALGIPPDKHDVTDGLTCLDNWQVLGQSAQEGNNLWRRRV
                     WLYGEKSHRTALLLDYSHGSKNFPQHFITGQVCQGALAFFPGASPLRARVVEPFTRGE
                     RFPLAELPLPDALHDMAQRLSANPWQWPLPLRVSEILIYPHESGWRFVSTQGQHLPTH
                     LSSDEGWRLLAASGGAPFMLMGEWDGQQLTPCSAWLAGSVIWATGGAQ"
     gene            complement(20807..22225)
                     /gene="gltD"
                     /locus_tag="PN977_002462"
     CDS             complement(20807..22225)
                     /gene="gltD"
                     /locus_tag="PN977_002462"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit GltD"
                     /protein_id="HDI6042485.1"
                     /translation="MSQNVYQFIDLQRVDPPKKPLKIRKIEFVEIYEPFSEGQAKAQA
                     DRCLSCGNPYCEWKCPVHNYIPNWLKLANEGRIFEAAELSHQTNTLPEVCGRVCPQDR
                     LCEGSCTLNDEFGAVTIGNIERYINDKAFEMGWRPDMSGVKQTGKKVAIIGAGPAGLA
                     CADVLTRNGVKAVVFDRHPEIGGLLTFGIPAFKLEKEVMTRRREIFTGMGIEFKLNTE
                     VGRDVQLDDLLSDYDAVFLGVGTYQSMRGGLENEDADGVYAALPFLIANTKQLMGFGE
                     TNDEPFVSMEGKRVVVLGGGDTAMDCVRTSVRQGAKHVTCAYRRDEENMPGSRREVKN
                     AREEGVEFKFNVQPLGIEVNGNGKVSGVKMVRTEMGEPDAKGRRRAEIVAGSEHIVPA
                     DAVIMAFGFRPHSMEWLAKHSVELDSQGRIIAPEGNDNAFQTSNPKIFAGGDIVRGSD
                     LVVTAIAEGRKAADGIMNWLEV"
     gene            complement(22238..26698)
                     /gene="gltB"
                     /locus_tag="PN977_002463"
     CDS             complement(22238..26698)
                     /gene="gltB"
                     /locus_tag="PN977_002463"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HDI6042486.1"
                     /translation="MLYDKSLERDNCGFGLIAHIEGEPSHKVVRTAIHALARMQHRGA
                     ILADGKTGDGCGLLLQKPDRFFRIVAQERGWRLAKNYAVGMLFLNKDPELAAAARRIV
                     EEELQRETLSIVGWRDVPTNEGVLGEIALSSLPRIEQIFVNAPAGWRPRDMERRLFIA
                     RRRIEKRLEADKDFYVCSLSNLVNIYKGLCMPADLPRFYLDLADLRLESAICLFHQRF
                     STNTVPRWPLAQPFRYLAHNGEINTITGNRQWARARTYKFQTPLIPDLHDAAPFVNET
                     GSDSSSMDNMLELLLAGGMDIIRAMRLLVPPAWQNNPDMDPELRAFFDFNSMHMEPWD
                     GPAGIVMSDGRFAACNLDRNGLRPARYVITKDKLITCASEVGIWDYQPDEVVEKGRVG
                     PGELMVIDTRSGRILHSAETDDDLKSRHPYKEWMEKNVRRLVPFEDLPDEEVGSRELD
                     DDTLASYQKQFNYSAEELDSVIRVLGENGQEAVGSMGDDTPFAVLSSQPRIIYDYFRQ
                     QFAQVTNPPIDPLREAHVMSLATSIGREMNVFCEAEGQAHRLSFKSPILLYSDFKQLT
                     TMKEDHYRADTLDITFDVTKTTLEATVKELCDKAEKMVRSGTVLLVLSDRNIAKDRLP
                     VPAPMAVGAIQTRLVDQSLRCDANIIVETASARDPHHFAVLLGFGATAIYPYLAYETL
                     GRLVDTHAIAKDYRTVMLNYRNGINKGLYKIMSKMGISTIASYRCSKLFEAVGLHDDV
                     VGLCFQGAVSRIGGASFEDFQQDLLNLSKRAWLARKPISQGGLLKYVHGGEYHAYNPD
                     VVRTLQQAVQSGEYSDYQEYAKLVNERPATTLRDLLAITPGENAVNIADVEPASELFK
                     RFDTAAMSIGALSPEAHEALAEAMNSIGGNSNSGEGGEDPARYGTNKVSRIKQVASGR
                     FGVTPAYLVNADVIQIKVAQGAKPGEGGQLPGDKVTPYIAKLRYSVPGVTLISPPPHH
                     DIYSIEDLAQLIFDLKQVNPKAMISVKLVSEPGVGTIATGVAKAYADLITIAGYDGGT
                     GASPLSSVKYAGCPWELGLVETQQALVANGLRHKIRLQVDGGLKTGVDIIKAAILGAE
                     SFGFGTGPMVALGCKYLRICHLNNCATGVATQDDKLRKNHYHGLPFKVTNYFEFIARE
                     TRELMAQLGVTRLVDLIGRTDLLKELDGFTAKQQKLALSKLLETAEPNAGKALYCTEN
                     NPPFDNGLLNAQLLQQAKPFVDERQSKTFWFDIRNTDRSVGASLSGYIAQTHGDQGLA
                     ADPIKAYFNGTAGQSFGVWNAGGVELYLTGDANDYVGKGMAGGLIAIRPPVGSAFRSH
                     EASIIGNTCLYGATGGRLYAAGRAGERFGVRNSGAITVVEGIGDNGCEYMTGGIVCIL
                     GKTGVNFGAGMTGGFAYVLDESGDFRKRVNPELVEVLSVDDLAIHEEHLRGLITEHVQ
                     HTGSQRGEEILANWSTFATKFALVKPKSSDVKALLGHRSRSAAELRVQAQ"
     gene            27372..28301
                     /locus_tag="PN977_002464"
     CDS             27372..28301
                     /locus_tag="PN977_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562163.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HDI6042487.1"
                     /translation="MQLQKLVNMFGGDLTRRYGQKVHKLTLHGGFSCPNRDGTIGRGG
                     CTFCNVASFADEAQQHRSIAEQLAHQANLVNRAKRYLAYFQAYTSTFAEVQVLRSMYQ
                     QAVSQANIVGLCVGTRPDCVPDAVLDLLCEYKDQGYEVWLELGLQTAHDKTLHRINRG
                     HDFACYQRTTQLARERGLKVCSHLIVGLPGEGQAECLQTLERVVETGVDGIKLHPLHI
                     VKGSIMAKAWEAGRLNGIELEDYTLTAGEMIRHTPPEVIYHRISASARRPTLLAPLWC
                     ENRWTGMVELDRYLNEHGVQGSALGRPWLPPTA"
     gene            28397..>29493
                     /locus_tag="PN977_002465"
     CDS             28397..>29493
                     /locus_tag="PN977_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HDI6042488.1"
                     /translation="MKQIRLLAQYYVDLMMKLGLVRFSMLLALALVVLAIVVQMAVTM
                     VLHGQVESIDVIRSIFFGLLITPWAVYFLSVVVEQLEESRQRLSRLVQKLEEMRERDL
                     SLNVQLKDNIAQLNQEIAVREKAEAELQETFGQLKIEIKEREETQIQLEQQSSFLRSF
                     LDASPDLVFYRNEDKEFSGCNRAMELLTGKSEKQLVHLKPADVYSPEAAAKVIETDEK
                     VFRHNVSLTYEQWLDYPDGRKACFEIRKVPYYDRVGKRHGLMGFGRDITERKRYQDAL
                     ERASRDKTTFISTISHELRTPLNGIVGLSRILLDTELTAEQEKYLKTIHVSAVTLGNI
                     FNDIIDMDKMERRKVQLDNQPVDFTSFLADL"
BASE COUNT         6644 a         7854 c         8017 g         6978 t
ORIGIN      
        1 cattgagctg aatactgatt ttctgtgcct gattaatcac atggttgttg gtcagcacat
       61 agcctttact ggcgttgatg atgacaccgg agcctaaacc ttcgaagggt tgtgcaggtt
      121 gatccggtaa atcatcacca aaaaactttt tgaattcttc cgggattttc tgtccctgac
      181 tggccgttcc ttccacccgt acgctcacca ctgccggaag cactttttcc agcattgggg
      241 ccagactggg gagaggggcc tgatcggcaa cctggcctgg aatcgacgca acggcctgaa
      301 atgacgccga gagagttaac ccgacactta acgctaatgc actcaacagc tgggtttgtt
      361 ttttcattat tcctgctctc gtacctgaat gataagaaaa gagattcaaa acagtttgat
      421 gttattgaat tttcagacgg ctaacaatga gacaccagat taaatgatag tcggggaggg
      481 agagaagagg agggcgcaat ggctgcgccc gaaaaataaa ttagtcgcgc ttcgcgccag
      541 tacgcagcag gccggatgcg ccttcagaat agtcgcgagg catttgcacc ggtgcctgat
      601 cgttgctggc ttcagactct gccagacgat tacggaacgg gtttgcttca gcagacagtt
      661 ccggcagcag gctgctggag cttttcgcca tgtgttgata cagctggcga tagtcgtgcg
      721 ccatggtatc cagtaattcc gcgctgcggg caaagtggct aaccagctct tcgcgatact
      781 cgtccagttc agctttattc ttttccagtt cgtactgcaa cgcctgttgc tggcgtagtt
      841 tacgattacc aaaacgcatg gccacagcac caataatgat gccgacgact aacccaatta
      901 gcgcatattc ccaggtcatg aacatctccc gttgtctttt gtttccgtag ggtgttggct
      961 tcaggctcca tgcctgcggc tgattatgcc actataaccg ttaattccac agaagtggaa
     1021 tcccgactgc atatcgcgta gtgtagaacg gccttttttt cgtcaaccgt gaacaacggc
     1081 gcaccgatta ttcaaggaat aacaataaga tcatgcaaag cgttacccca acatcgcaat
     1141 acctgaaggc gctcaatgaa ggcagccatc aacccgacga cgttcaaaaa gaggccgtca
     1201 gccgcctgga aattatttat caggaactca tcaatagcac gcctccagcc cctaggacga
     1261 gtgggctaat ggcgcgggtc ggtaagctgt ggagtaaacg cgaagacaca aagcatacgc
     1321 cagtgcgtgg cttatatatg tggggcggtg taggacgcgg gaaaacctgg ctgatggacc
     1381 ttttctatca aagcctgccg ggagagcgga aacagcgtct gcactttcac cgttttatgc
     1441 tgcgggtgca cgaagagctg accgaattac aaggccaaag cgatccgctg gaaattattg
     1501 ccgatcgctt taaagcagaa actgacgtgc tctgttttga cgaatttttt gtttctgata
     1561 ttaccgatgc catgctactt ggcggtctga tgaaagccct gttcgcccga ggtattaccc
     1621 tggtagcgac gtcaaatatt ccgccggacg aactttatcg aaatggccta caacgtgcgc
     1681 gttttttgcc tgcaatcgat gccattaaac agcattgtga tgtaatgaac gtggacgctg
     1741 gtgttgatta tcgactgcgt acactcactc aggcgcatct gtggctttcg cccctcaacg
     1801 atgaaacccg ggcgcaaatg gataaactat ggttggcgct ggcgggggcg aaacgagaaa
     1861 attcaccgac attagaaatc aaccatcggc cattggcgac aatgggcgtc gagaaccaga
     1921 cgctggcggt ctcttttact acgctgtgcg tcgacgcccg cagtcagcat gactatattg
     1981 cgctctcacg cctctttcac acggtcatgt tgtttgatgt accagttatg acgcggttga
     2041 tggagagcga agcgcggcgc tttattgcgc tggtggatga gttttacgag cgccatgtca
     2101 aattagtggt gagtgcagaa gtgccgctat atgacattta tcagggcgag cggctgaaat
     2161 ttgagttcca gcgttgcctg tcacgtctgc aagagatgca aagcgaagag tatctgaagc
     2221 gcgagcattt agcgggttaa aacctgccac aaatcacaaa aaggggtcga tctttgaccc
     2281 cgacttctct ataatcctgc gaccccacgt tacaagaaag tttttttccc aaaacttttt
     2341 gtgtgctggc ataggctatt cgaaggggta ggtttgccgg actttgtcgt gtgaacctca
     2401 acaattgaag acgtttgggt gttcaccaac gtgtaactat ttattgggta agcttttaat
     2461 gaaaactttt acagctaaac cagaaaccgt aaaacgcgac tggtatgttg ttgacgcgac
     2521 cggtaaaact ctgggccgtc tggctactga actggctcgt cgcctgcgcg gtaagcacaa
     2581 agcggaatac actccgcacg tagataccgg tgattacatc atcgttctga acgctgacaa
     2641 agttgctgta accggcaaca agcgtactga caaagtgtac tatcaccaca ccggccacat
     2701 cggtggtatc aaacaagcga cctttgaaga gatgattgct cgccgtcctg agcgtgtgat
     2761 tgaaatcgcg gttaaaggca tgttgccaaa aggcccgctg ggtcgtgcta tgttccgtaa
     2821 actgaaagtt tacgcgggta acgagcacaa ccacgcggca cagcaaccgc aagttcttga
     2881 catctaatcg ggattatagg caatggctga aaatcaatac tacggcactg gtcgccgcaa
     2941 aagttccgca gctcgcgttt tcatcaaacc gggcaacggt aaaatcgtaa tcaaccaacg
     3001 ttctctggaa cagtacttcg gtcgtgaaac tgcccgcatg gtagttcgtc agccgctgga
     3061 actggtcgac atggttgaga aactggacct gtacatcacc gttaaaggtg gtggtatctc
     3121 tggtcaggct ggtgcgatcc gtcacggtat cacccgcgct ctgatggaat acgacgagtc
     3181 cctgcgttct gaactgcgta aagctggctt cgttactcgt gacgctcgtc aggttgaacg
     3241 taagaaagtc ggtctgcgta aagcacgtcg tcgtccgcag ttctccaaac gttaattggc
     3301 ttctgctccg gcagaaaaca atttgcgaaa aaacccgctt cggcgggttt ttttatggct
     3361 aaattctgaa tcagcgtaaa aaatggaaag ttgctttttg ctgccgtctg acaacaggca
     3421 aaacaaaaac cacatcgcca ataagggact aagtcaacta tttcagacta aagcgcatct
     3481 ctttttcccc atttccggca tcgactcacc acaaaggtcg caaaatctgg taaactatca
     3541 tccaattttc tgcccaaatg tcgggtattg ctcatttttt gtttgatttt cgaacaaaga
     3601 gagtcgttcc ttactgggta acacaacttc tgactggcca cctggtggct ggtagcagta
     3661 aaaattctga ctatacctgg aggttttcat ggctgtcgct gccaacaaac gttcggtaat
     3721 gacgctgttt tccggtccta ctgacatcta tagccatcag gtccgcattg tgctggctga
     3781 gaaaggtgta agtttcgaga tcgaacacgt ggaaaaggac aatccgcctc aggatctgat
     3841 tgacctcaac ccgaatcaga gcgttccgac cctggtggat cgtgagctga ccctgtggga
     3901 atctcgcatc attatggaat atctggatga gcgtttcccg catccgccac tgatgcctgt
     3961 ttacccggta gctcgcggtg aaagccgtct gtacatgcat cgcatcgaaa aagactggta
     4021 cacgctgatg aacaccatca tcaacggttc agcttctgaa gcagatgccg cacgtaagca
     4081 actgcgcgaa gaactgctgg cgattgcgcc ggtcttcggt cagaagccgt acttcctgag
     4141 cgatgagttc agcctggtcg attgctacct tgctccgctg ctgtggcgtt tgccgcaact
     4201 gggcatcgag ttcagcggcc cgggggcgaa agagctgaaa ggctatatga cccgcgtctt
     4261 tgagcgtgac tctttccttg cttctttaac tgaagctgaa cgtgaaatgc gtctgggccg
     4321 gagttaatct gtatggattt gtcacagcta acaccacgtc gtccctatct gctgcgtgca
     4381 ttctatgagt ggttgctgga taaccagctc acgccgcacc tggtggtgga tgtgacgctc
     4441 cctggcgtgc aggttcctat ggaatatgcg cgtgacgggc aaatcgtact caacattgcg
     4501 ccgcgagctg tcggcaatct ggaactgtcg aatgatgagg tgcgctttaa cgcgcgcttt
     4561 ggtggcattc cgcgtcaggt ttctgtgccg ctggctgccg tgctggctat ctacgcccgt
     4621 gaaaatggcg caggcacgat gtttgagcct gaagctgcct acgatgaaga tgccagcatc
     4681 atgaatgatg aagaggcatc ggcagacaac gaaaccgtta tgtcggttat tgatggcgac
     4741 aagccagatc acgatgatga cactcatcct gacgatgaac caccgcagcc accacgcggt
     4801 ggtcgaccag cattacgcgt tgtgaagtaa tactaaaaca ggccctggcg gcctgttttg
     4861 tctttttaat cgcttaataa aatagcgtga tagtggcaat catattcacc acgaaaccga
     4921 ctgccatggg gatcgctgtc cgcttcacta ccgcaaaagg cgaaacgcct gcaattcctg
     4981 acgtaacgac caccaccgca gtaatcggcg aaacagcgcg cgctagcgtc gtggcaaaat
     5041 gcattggcat aatcattaca accgctggta catgtagtcc ggctgcgata ttcggaataa
     5101 gactggcaaa tgacataaac ggcgcattgc ctgagcccat cacaatggca caaatggcaa
     5161 tgaccagcgc cataataatc atcacgccaa taccgcccag accagaatgc tccgcccccc
     5221 tgataaccgc atcgacagtg ccaatcgtcg ttaagccttt cgcaaatatt tcacccgcga
     5281 ccaccagcgt taccacgttg gcaaactgcg tacccatgcc gtcaaaaaac gcctgcacat
     5341 cgtccattgt ttcgcgcaag ttatgcttgc ggaagaactc aacaaacatc gtcaccgtca
     5401 aactcagtaa catcaccacc accagatgca gttctgactg catcagcccg acgtgggcaa
     5461 ggaacagcga gccgagcatc aggattaacg gcatcaccgg taaaatggcg taatagagcg
     5521 gcgggacatt atcgagagct ttttgctctg cctgttcgtg attgatatgt ttatcttttt
     5581 tgtcgaaagc gcgttgcacg aaaaagtggg agatcgccac cgagataatg acgcaagagg
     5641 cgaccggaag ctggtagtgg aagaagtaag tggcaatttt cattcctgcg acctgggcag
     5701 caaaaatgga gttcgtttcc agaatccccc actcaatgga catggtggtt gcgataaccg
     5761 ccaccgcaga gagacgactt actcccagac tcaccagcgt cggaaataag gtgaccatca
     5821 gcaacatacc cagaccggag gcgctggtaa taaactgcgc catgatttgg ccgatgacgt
     5881 aagttgccga cagaataata tacggcgagc gaatgagttt taacgggcgg cttaacaagc
     5941 tcaccatcgc gcgacttgcc cctatgcgct ccatatagcg ggcataaccg cccactgcca
     6001 taatcgacag ccccaatccg gcaatgcggt tggacagcat gcgcaggatt tcattataaa
     6061 tatcgacgat caagtagccg ctgcttttgg cgggatcgag cacaccaccg aaccctaacc
     6121 agacaccgca catcatcagg aagatacctg cggcagccag caccacctga ggtttgtagt
     6181 ttttcaggat caaatagccc atcgtaatta atacgatgac agatataatt atgccgaaca
     6241 ttgttgaact ccgtgtcaaa agaaaacggt caatcccata aacggcagat tgaaaacaat
     6301 gatgttatat tttttgcaag gctatttatg gtgcggatgt cgtgttttta attgtagatg
     6361 aggtgatttt tcattaaaaa atatgcgctt atgattattt tgtaagaaca cattcataat
     6421 attcataata cttgttaata gtcttatgaa taattcaatc gggatgtttt tatctgcgtt
     6481 acattaattt ttcgcaatag ttaattattc agttaattat agtaatgatg aggcacaaag
     6541 agaaaacccc gccatttccc cctactttca atcctgtgat aggatgtcac tgatgatgtt
     6601 aatcacactg accttacaga atgggcctta tgaacgcatt tgattcgcaa accgaagatt
     6661 cttcacctgt aattggccgc aacttacgca gccgcccgct ggcgcgtaaa aaactctccg
     6721 aaatggtgga agaagagctg gaacagatga tccgccgtcg tgaatttggc gaaggtgaac
     6781 aattaccgtc tgaacgcgaa ctgatggcgt tctttaacgt cgggcgtcct tcggtgcgtg
     6841 aagcgctggc agcgttaaaa cgcaaaggtc tggtgcagat aaacaacggc gaacgcgctc
     6901 gcgtctcacg tccttctgcg gacaccatca tcggcgagct ttccggcatg gcgaaagatt
     6961 tcctttctca tcctggcggg attgcccatt tcgaacaatt acgtctgttc tttgaatcca
     7021 gcctggtgcg ctatgcggct gaacacgcta ccgatgagca aatcgatttg ctggcaaaag
     7081 cactggaaat caacagtcag tcgctggata acaacgcggc attcattcgt tcagacgttg
     7141 atttccaccg cgtgctggcg gagatccccg gtaacccaat cttcatggcg atccacgttg
     7201 ccctgctcga ctggctgatc gccgcacgtc caaccgtggc tgatcaggct ctgcacgagc
     7261 ataacaacgt tagctatcaa cagcatattg cgatcgttga cgcgatccgc cgccacgatc
     7321 ccgatgaagc cgatcgcgcg ttgcaatcgc atctcaacag cgtctctgct acctggcacg
     7381 ccttcggtca gaccaccaac aaaaagaaat aatgccactt tagtgaagca gatcgcatta
     7441 taagctttct gtatggggtg ttgcttaatt gatctggtat aacaggtata aaggtatatc
     7501 gtttatcaga caagcatcac ttcagaggta tttatggcaa cgaatttacg tggcgtaatg
     7561 gccgcactcc tgactccttt tgatcaacaa caagcactgg ataaagcgag tctgcgtcgc
     7621 ctggttcagt tcaatattca gcagggcatc gacggtttat acgtgggtgg ttctaccggc
     7681 gaagcctttg tacaaagcct ttccgagcgt gaacaggtac tggaaatcgt cgccgaagag
     7741 gcgaaaggca agattaaact catcgctcac gtaggttgcg tcagcaccgc cgaaagccaa
     7801 caacttgcgg catcggctaa acgttatggc ttcgatgccg tctccgccgt cacaccgttc
     7861 tactatcctt tcagctttga agaacactgc gatcactatc gggcaattat tgattcagcg
     7921 gatggtttgc cgatggtggt gtacaacatt ccggcgttaa gtggcgttaa actgagcctg
     7981 gatcagatca acactctggt tacgttgcca ggtgttggcg cgctgaaaca gacctctggc
     8041 gatctctatc agatggagca gatccgtcgt gaacatccgg atctggtgct ctataacggt
     8101 tacgacgaaa tcttcgcctc tggtcttctg gcgggcgctg atggtggtat cggtagtacc
     8161 tacaacatca tgggctggcg ttatcagggc attgttaagg cgctgaaaga aggcgatatc
     8221 cagaccgcgc agaagctgca aaccgaatgt aataaagtca ttgatttact gatcaaaacg
     8281 ggcgtattcc gcggcctgaa aacggtcctc cattatatgg atgtcgtttc tgtgccgctg
     8341 tgccgcaaac cgtttggtcc ggtagatgaa aaatatctgc cagaactgaa ggcgctggcc
     8401 cagcagttga tgcaagagcg cgggtgagtt ttttcccctc gctcgcccct accgggtgag
     8461 gggaaataaa cgcatctgta ccctacaatt ttcataccaa agcgtgtggg catcgcccac
     8521 cgcgggagac tcacaatgag tactacaacc cagaatatcc cgtggtatcg ccatcttaac
     8581 cgcgcacaat ggcgggcatt ttccgctgcc tggttgggat atctgcttga cggttttgat
     8641 ttcgttttaa tcgccctggt actcaccgaa gtacagggtg aattcgggct gacgacggtg
     8701 caggcggcaa gtctgatctc cgcagccttt atctctcgct ggttcggcgg cctgatgctc
     8761 ggcgctatgg gtgaccgcta cgggcgtcgt ctggcaatgg tcaccagcat cgttctcttc
     8821 tcggccggga cgctggcctg cggctttgcg ccaggctaca tcaccatgtt tatcgctcgt
     8881 ctggtcatcg gcatggggat ggcgggtgaa tacggttcca gcgccaccta tgtcattgaa
     8941 agctggccaa agcatctgcg taacaaagcc agtggttttc tgatttccgg cttctcagtg
     9001 ggggccgtcg ttgccgctca ggtctatagc ctggtggttc cggtctgggg ctggcgtgcg
     9061 ctgttcttta tcggcatttt gccaatcatc tttgctctct ggctgcgtaa aaacatcccg
     9121 gaagcggaag actggaaaga gaaacacggg ggtaaagcac cggtacgcac gatggtagac
     9181 attctttacc gtggtgaaca tcgcattgcc aatatcgtaa tgacactggc ggcggctact
     9241 gcgctgtggt tctgcttcgc cggtaacctg caaaatgccg cgatcgtcgc tgttcttggg
     9301 ctgttatgcg ccgcaatctt tatcagcttt atggtgcaga gtacgggcaa acgctggcca
     9361 acgggcgtaa tgctgatggt ggtcgtgttg tttgctttcc tctactcatg gccgattcag
     9421 gcgctgctgc caacgtatct gaaaaccgat ctggcttatg acccgcatac tgtcgccaat
     9481 gtgctgttct ttagtggctt tggcgcggcg gtggggtgct gcgtcggtgg cttcctcggt
     9541 gactggctgg gaacccgcaa agcgtacgtt tgtagtctgc tggcctcgca gctgctgatt
     9601 attccggtat ttgcgattgg cggcgcaaac gtctgggtgc tcggtctgtt actgttcttc
     9661 cagcaaatgc ttggacaagg gatcgccggg atcttaccga aactgattgg cggttatttc
     9721 gataccgacc agcgtgcagc gggcctgggc tttacctaca acgttggcgc attgggcggc
     9781 gcactggccc caatcatcgg cgcgttgatc gctcaacgtc tggatctggg tactgcgctg
     9841 gcatcgctct cgttcagttt gacgtttgtg gtgatcctgc tgattggtct ggatatgcct
     9901 tctcgcgttc agcgttggct gcgcccggaa gcattgcgta ctcatgacgc tatcgacggc
     9961 aaaccattta gcggtgccgt gccatttggc agcgccaaaa acgatttagt caaaaccaaa
    10021 agttaatcct gttgcccggt ctatgtaccg ggcctttcgc taagggaaga tgtatgtcgt
    10081 tacttgcaca actggatcaa aaaatcgctg ctaacggtgg cctgattgtc tcctgccagc
    10141 cggttcctga cagcccgctc gataaacccg aaatcgtcgc agccatggca ttagcggcag
    10201 agcaggcggg cgcggttgcc attcgcatcg aaggtgtggc aaatctgcaa gccacgcgtg
    10261 cggtggtgag cgtgccaatt atcgggattg tcaaacgcga tctggaggat tctccggtac
    10321 gtatcacggc ctatattgaa gatgtcgatg cgctggcgca ggcgggcgcg gacattatcg
    10381 ccattgacgg caccgaccgc ccgcgtccgg tgcctgttga aacgctgctg gcacgtatcc
    10441 atcatcacgg tttactggcg atgaccgact gctcaacgcc ggaagacggc ctggcatgcc
    10501 aaaagctggg agccgaaatt attggcacta cgctttctgg ctataccacg cctgaaacgc
    10561 cagaagagcc ggatctggcg ctggtgaaaa cgttgagcga cgccggatgt cgagtgattg
    10621 ccgaagggcg ttacaacacg cctgctcagg cggcggatgc gatgcgccac ggcgcgtggg
    10681 cggtgacggt cggttctgcc attacgcgtc ttgaacacat ttgtcagtgg tacaacacag
    10741 cgatgaaaaa ggcggtgcta tgaccacact ggcgattgat atcggcggta ctaaacttgc
    10801 cgccgcgctg attggcgctg atgggcagat ccgcgatcgt cgtgaacttc ctacgccagc
    10861 cagccagaca ccagaagcct tgcgtgatgc cttatccgca ttagtctctc cgttgcaagt
    10921 tcatgcgcag cgggttgcta tcgcttcaac cggaattatt cgcgacggca gcctgctggc
    10981 gctgaatccg cataatctcg gtggattgct acactttccg ttagtcaaaa ctttggaaca
    11041 acttaccgat ttgccgacca ttgccattaa cgacgcgcag gccgcagcat gggcggagta
    11101 tcaggcgctg gaaggcgata taaccgagat ggtgtttatc accgtttcca ctggcgtcgg
    11161 cggcggtgta gtgagcggcg gcaaactgct taccggccct ggcggtctgg cggggcacat
    11221 cgggcatacg cttgccgatc cgcatggtcc agtctgcggc tgtggacgca caggttgcgt
    11281 ggaggcgatt gcttctggtc gcggcattgc agcggcagcg cagggggagt tggctggtgc
    11341 gaatgcgaaa actattttca cgcacgctgg gcagggcgat gagcaggcgc agcaactgat
    11401 tcaccgttcc gcacgtacgc ttgcaaggct gatcgctgat attaaagcca caactgattg
    11461 ccagtgcgta gtggtcggtg gcagcgttgg tctggcagaa gggtatctgg cgctggtgga
    11521 aacgtatctg gcgcaggagc cagcggcatt tcatgttgat ttactggcgg cgcactaccg
    11581 tcatgatgca ggtttacttg gggctgcgct gttggcccag ggagaaaaat tatgatgatg
    11641 ggtgaagtac agtcattacc gtctgccgga ttacatcctg cgttacagga cgcgttaacg
    11701 ctggcattag ctgccagacc gcaagaaaaa gcgccgggtc gttacgaatt acagggcgac
    11761 aatattttta tgaatgtcat gacgtttaac actcaatcgc ccgttgagaa aaaagccgaa
    11821 ttgcacgaac aatacattga tatccagctg ttattaaacg gtgaggaacg gatcctgttt
    11881 ggcatggcag gcactgcgcg tcagtgtgaa gagttccacc atgaagatga ttaccagctt
    11941 tgcagcgcca ttgagaatga gcaaaccatc atcttaaaac cgggaatgtt tgccgtgttt
    12001 atgccgggag aaccgcataa accaggatgc gttgtcggcg aacctgatga gattaaaaag
    12061 gttgtggtga aggttaaggc tgatttaatg gcttaaacgt attcgctcag gcagggggcg
    12121 ctggttggga actcaacgtt ggcatggttt cgctgctctt gtgtagatgt gttgcagtta
    12181 tttgcgacat tccgccggag tagcggaact atggaaaacg gcaattggcg ctcttatccc
    12241 ttagctttgt aaggcttaat tttggtagaa tggggaatcg tttaacggaa aaaaacaatc
    12301 agatgatttc ttttctatcc gatgaatttg aggaatttcc gcagggagat tttttaaatc
    12361 atctctatag gttttcggta gaccatcatc attttatcgt gatatttagc cctattgata
    12421 ttagcgatgc ggagtaccat caataccgta gcgaggaagt tggatttgaa atcccaacta
    12481 attgttttga tgtgaaattt gataggcaag aaaactttga tagtggtaac ttttacatgc
    12541 cccccgccgc aactgaatgc tcgaggcgca gacgctttac tgatgaactc gcagaggcgc
    12601 tagcaacgat tattgaaaag cattacatca tttatcatgc cagggcttat cttgccatcg
    12661 cagagaatga caagttgaaa cgttactatg accggatttt acacaatgcc ccggctaatg
    12721 tagcatatag agtaatcaaa gatgttggag aggaggaacg aggttatgcc atacaaaccg
    12781 aatgtttccg aacttaagaa aatgactgtt gcagagcgca agaaagaagc catgcgccag
    12841 cttaaagcat cctttgagcg cgccagcatt gaagggactt tgcataaacg ctctgcaaag
    12901 tctgttgcgt aatgaaagat ttcaataacc cgctgcggcg ggcctttttt tgccctcaga
    12961 aaatcccctc tgacctgcct cagggattat atacaacctt cagttaatta gctgcgcacc
    13021 atacgcggcc cgagccttat cgccttgttc aaattcgaca attcgtcggc caatttcccc
    13081 gaacgtcgcg gtgctaagta ccgtatccgt ccgggcgtat ccggccagaa ttttcaggcg
    13141 gtttctcttg ctgtcactac gccattttgc gcagcccaca gattcacatc ttccttacga
    13201 atggcggcgg ccatcatgcc ggggaataaa tctggggtac aggcaaagga gggaataccc
    13261 atggcggcga gttttcctgc cagtgaccgg tcgtaaaacg gcgcaccttc atcgcttaag
    13321 gccagcaggg taaccacctg tacgccggac tgaatcaact cactggcgcg ttgcaacaga
    13381 ttgcgttcca ccccgccttc gtagaggtcg gaaatcagca ccagaatggt attgcgcgga
    13441 tcgcgaatca aagactggca gtagcccacc gcgcggttga tgtcggtgcc gccacccagc
    13501 tgtacgccaa acagcagctc taccggatcg tcgagcttct ctgtcatatc aacgactgcg
    13561 gtgtcgaaaa ccaccagatg cgtttttacc gccgggagcg atgccattac tgcgccaaaa
    13621 atactggaat agaccaccga ggaggccatc gacccactct ggtcgataca taaaatgatc
    13681 tccttttgcg tgcgttgaga cttgcgcccg tagccgatca gcgtttcagg aacaatactt
    13741 ttatactctt cctgccagtg gcgcaggttg gcgcgaatgg tgcgctgcca gtcaatttcc
    13801 gcgtggcgcg ggcgacgatt acgtaccgca cgattaagag cgccgcttac cgcgctacgc
    13861 attggctctt caagctgttt catcaattgc tcgacaactt tacgcaccac aagcctggcg
    13921 gtttctttgg ttttggccgg aattacgcca ttcagcgaca tcagcgtgga gaccagatga
    13981 acatcaggct ggacgtttgc cagcatctcc ggctccagca acatactgtg caaattaaga
    14041 cgttcaaagg cgtctttttg catcacctga accacagaag aagggaaata ctggcgaata
    14101 tcacccagcc agcgagcgac tttgggtgac gatccacccg tgccgccgcg taatccatgt
    14161 ttaccctctg gctgatacag cgccgccagc gcggcatcaa tcgcggactc tctggcatcc
    14221 agcgccacgt tacaggcatt gtcggcatct tctccaagga tcagacgcca gcggcgcagc
    14281 cgttccattt cgttactcga catgattttc cctttcgtgt ggtggcaaag ccagaatgcg
    14341 gcgcagtagc ggcagcatta acgcggcacg ctgttcatcc catataccgc tggtctcttc
    14401 ttgctgcgcg acacgctcgc cctgcgcggc gcgttcgcct aactggcggc gttccgggct
    14461 ggaaaaacgg ccgaatgtcc gccgcagcat cggcaggatc cgggtgaagt gattgtcgtt
    14521 caggccgctt agccaggcat cgatcaactg ccataattcg ctattgtgca gtagcaccat
    14581 cgcgctgtcg ttgaggaaac cttccgccca tgcggcactt tccgccgggg gattggctgg
    14641 cgatagcgcc aggttcatca gcgtgctgat ccgttctgtc gttaacgttt gcagatcaaa
    14701 caacaggcgc gtcgccgttc cgcgaagcag cgcgtgtgcg gtactgccct ctgccagcac
    14761 catcaacgcc tgcaaccagc ccgccaatag atcttcgtta tccagcaacc gcagcgcggc
    14821 gtgggcgtcg ataacttttt ctctcatact ggcggcgctg tcgtcgttga gcgccgaaca
    14881 ggctccgggt agggcgatgg cggcgcgcgg ggcgaggctc atcagtacct gcattaccat
    14941 accagagtcg gtctggcgca cgttgccgta gcggacaatc gccaccagtg gtgccagggc
    15001 ttccagcaac tgttcgatat tgccctgagt ggcagccagt gattccagtg tattggcgat
    15061 gggggcaatc gcggtctgta aatcggccag tagcacctgt tggataagct ttgccagttc
    15121 cggcagcgtg ctggcgtatt gcgccctcac aatggcatag cgggagctgg cctgctcaat
    15181 gctgttaccc caacggctgg cggtaatgat gttgattgcc agtgccggtt cccagcgcag
    15241 cgaccagact tcgtggaacg tccctttgct gttatttcct tgtccagcca gacggcccca
    15301 ggaaatgtcg agtaaggtca ggcgatgcag taaatggcta cgcgccagat cgccgggttt
    15361 gcgcagatca agatccagca ctttttcgct ggcttctgcc ttcaggcgca gcgttttttg
    15421 ctgttgcgta atgtcgcgtt ggagcggcac gacgggcgta tcatccggta cgcttcccag
    15481 cgcgtcgccg acaattaact tctggcgaat catctgcatt ggcgcgcttt cgcccataca
    15541 cactacggtt tgcagcgctt cgcaaagttc gtcaagcccc ggctgcgggt gatggcgcat
    15601 aatcgccagc gtctgcgcca gccgcgccgc ttcgataatg tgggcgctgg aacagtcgag
    15661 atcggcttcg cgaaacagcc ttgccgcgcg tgacagccag ccgatatcgc ggtgagaagc
    15721 gccctgataa cgccacagat gatcgtacca ttccggtgac accacccctg cgccataacc
    15781 gctggcgcgg cttaacgcct cgtagctcca gggcgtccag gctgccgcga ccttgcactt
    15841 cggcaaattt ttcagcagtt cgttatcatt ctttgcgggc ggcaggtttt ccagcgccgg
    15901 aacgtgccac gcaccgcaga ctaccgcgat gcgcgagaag ccctcttttt tcgcctgacg
    15961 tagcgtcttg cgcatatacg cttcacgcag ttgatctctg gctgagtttc gggcttcggg
    16021 agttgcctgg cgtagggcaa tcatcgcctc gcgaatcgcc tcgaacagag acaaatcatc
    16081 ttcgcgttcc tctactctgt ggctccacca gctttcccca tcggtgtaac cggcggcgcg
    16141 gcccagccag tccagcggat cgccgtcggg actggcttca acggcatcat cgtcatcgct
    16201 ctctttttcg tcatcaagcg cgaactgatg actgatgggc aagtccataa agcgcacggc
    16261 gatattttgc tcaaaaccat agcgcagcgc ctgccattcc ggggagaacg ccgcaaaggg
    16321 gtaaaacgcg gcgtgatgtg agtcgtccgg ggcataaatc agtagcgcga ccggcggttg
    16381 catctgttca tgctgcataa aaggcaggat ggattcgccg tcgggcggcc cttccaccag
    16441 caggcagtcg ggctgtagcg tctccagcgc ctttcgcagg ctgcgcgcgc agccagggcc
    16501 atggtgacgg atgccaaaca aatgaacggt ttgttcagtc atagtggttg ccttgctgat
    16561 tacacaattt cgcggcaggc gcgatagaga tcgttccagt cgctgcgctc tttgactata
    16621 gtttccagat attcacgcca taccacgtcg tcctgcacgg gatcttttac caccgcgccg
    16681 ataaggcttg cggcgatatc ccgcggatgt agcacgccgt cgccaaaatg gcccgccagc
    16741 gccataccgt tgttgataac ggagatcgcc tccgcggtgc tgagagtact ggttggcgac
    16801 ttaattttcg ttttgccgtc aaccgtcagg ccgtttcgca gttcgcggaa gatttgcacg
    16861 actcgctgga tctctttaag cgctggcggt tcggcaggaa gtgccagcgc ctgacccagt
    16921 tcggcgacgc ggcgagtgac aatgttaatc tcttcttctt cgctggcggg tacgggcaaa
    16981 atgaccgtgt tgaaacgacg tttaagtgcg gaagaaagct cgttaacgcc tttatcccgg
    17041 ttgttggcgg tggcgataac gttaaagccg cgcgtcgcct ggacctcttc attcagttcg
    17101 gggataggca gcgttttttc cgacaggatc gtgatcagcg tgtcctgaac atcggcagga
    17161 atacgcgtca gctcttctac gcgggcaatc tgcccgcgtg ccatcgcccg catcaatggg
    17221 ctttctacca gcgccgaacg tgacggacct ttagagagca actccgcgta gttccagcca
    17281 taacgcattt gttcttcacc ggtgcctgcg gtgccctgaa taattaacgt tgagtcgccg
    17341 ctgatggcag ccgccagatg ttcagagacc caggatttcg cagtgccggg tacgccatac
    17401 aacagcagtg cgcgatcggt tgccagcgtg gcaacggcgg tttcgataat ccgcctgttg
    17461 ccgatatatt tcggcgtgac ctcaaaacca ttttccagtt gcccgcctaa cagataagta
    17521 gcaacggacc acggagaaat agcccagttt tccgggcgcg gatgagtatc gtttttttgc
    17581 agctcatcca gttcttctgc aaattgttgt tcggcatgtc ggcgtaaaac ggcggcagta
    17641 gcggtggaag tcataaagta gtttctccga caaaaaacga atgaagttga attcggtgcg
    17701 ccagcgtggc gctcagggca gtaaaggctg cctcgcaata aggcgtttgt tgtgcgtcat
    17761 gcggccactt gtcaatgaca tcattgagga cttcgggggc cagcaggcag gcgaaatcac
    17821 tcatcacatg gcgcagggcg tagtcaaagc gcgcggaatc atgtttcacc caacggtgca
    17881 gctccgtgac catttgctgg ctcaggcgca ggctaaagag cgacgcatcc agcggtaaca
    17941 gggcgatgta gcgttgtaaa tgttcgccgc ccattaccga gaacagtaac gtcaacactg
    18001 gctcgtgttc ttctacgggc aggcattgca gaagctcaaa ggcgttaatc ttcacgccag
    18061 caggggaatg aatgcttatt ccgacgggga gtagggcgag gaatgcctgc gcccactgtg
    18121 gatttttttc gcgcaggacg gcgtgatacc aggcgcgcag tagcgcctcc tgccagtcgg
    18181 ttttcccggc ccagcgcagc agttcaacgg gggtagcctg cagttgcgtt tgccaccagt
    18241 cgagcggaac cgctgcaact atctgataca gccaccatgc gcgctggccc aacttttcgc
    18301 ctttgggctt agtttcttcc agcgcatcgg ccttccactc tggtaagaac gcctccgggg
    18361 catcaagggc gacgcgcagc attttttctt tgccggataa gccttttagc ctttcaagta
    18421 aggtctcagg agcgggggat gaactgagca acggcgctaa ccgagacttc atgcgcgaga
    18481 tccaggcgct ttccggcaga caacacagta accgtgccgc agtttgccgc acctctttgc
    18541 tgcgatcgga taatgtctgt tccagaaagg cttcgtcgtg ttgggagatc cccacttcca
    18601 gcacagccag cagctgtgcg cgttcgcggg catccagcga actcatctcc tgcgccagcc
    18661 gatcgcgacc ctgttccggg gcgcgaatgc gcatttgcgt cagatattgc agacgctgtt
    18721 cggacgtgcc gtgcagccac atttcttcat ccagcggagc gtcagtggag gcgagaacat
    18781 agccccaatc ggcgttgatt tgcgccagcc agcacccgcg agcgcccaga acattggaca
    18841 ggatgccgcg tagagatggc ttttgcgctc cataattcag caatgctggt aacagcaggg
    18901 gcggaggaag aagttgacga cggaccaata gatcaagcgc cgaccacagc aaccgcggcg
    18961 gtccttctga cagcagcaga tgcagcagtc ccgcaaaagc cgggttgttt attggctcgg
    19021 cattttcttt atcgatttcc ggcagcggtg cggggtcaat cgcctgcggc acccagccag
    19081 ccagatggca gagcgccaga acgcccgccg agcgcagtag ctgttgctct gcggatgtgt
    19141 tattcgccag ttcagacagt aacagcccaa tctcactttg cgacgccgac ggtgtaaacg
    19201 cttttttgtc tgtgccgagt agcgctgtgc gggttaaatt ttgccattcg ttcattgtgc
    19261 gcctccggtt gcccaaatga cgctaccggc aagccaggcg ctacagggcg tcagctgctg
    19321 accatcccat tcccccatta gcataaacgg cgcgccgccg ctggccgcca gtaaccgcca
    19381 accctcgtcc gacgacaggt gcgtcggtaa gtgctggccc tgtgtcgaga cgaatcgcca
    19441 gccgctctcg tgcgggtaaa tcaatatttc gcttactcgc agcggcagag gccattgcca
    19501 gggattcgcg ctaagccgct gcgccatgtc atgcagcgcg tccgggaggg gtaattctgc
    19561 aagcgggaat ctttcaccgc gggtaaacgg ctccacaacc cgcgcccgta gcggtgatgc
    19621 gccagggaaa aaggcaagcg cgccctggca gacttgcccg gtgataaagt gctggggaaa
    19681 gtttttacta ccgtgtgaat agtccagcag tagcgcggtg cggtggctct tttctccgta
    19741 cagccagacc cggcgtcgcc acagattatt cccttcttgc gcgctttgac ctaagacctg
    19801 ccagttatcg aggcaggtta atccgtcagt gacgtcgtgt ttatccgggg gtattcccaa
    19861 tgccgcatgg agatcgctaa gttgtgccgg ggataacatt tgttgctggc gccaggcgtc
    19921 aataatcagc tgtagctgcc ccagttcttt gcacaggctc gatggccata cctcggcggt
    19981 agtaaaatga gtggtcagat tgttcaggcg agcggcaatt cccggtagtt gggcgtcgac
    20041 catgcgggcg gcgatcccgg caaaaggcaa ctgttgtgca gagatctgcg ccagaccgtg
    20101 gcggatcagg tcgtgcatcc actgttcgag gaaatccagc cccgccgtca ttttttggtt
    20161 gcgtgccgct tcccgtttgg cggcagcctg cgggtcggca gcttttgcct cagcctgctc
    20221 tttcttctct tctttgcgaa ccgcccgctg ctggcgcgag gttagccact cgcttaccca
    20281 ttccggcggc tcgccagcgg tgaaacttgc ggtgtgttgc acctggagaa aaagcaacgc
    20341 aaggctatgt ttacagggga atttacggct ggggcaggaa catttacagg ccagatcctg
    20401 tctgtcgatt cgtacctgat aagggcggga gccgctacct ttacactctc cccagaaggc
    20461 agcctcgcta tatccgagcg tcggccattt tgcgggagca accaggccac gagccgcttt
    20521 aaccgaggcg tcatcgggcg ccagggcttg cactgattca acggtcagtt ccattacaat
    20581 tctctattgg cgtggggaaa tcgatggggt caaggaggcg ttaagcaaag caggaggaga
    20641 ctgttttccg taacagcgta ttgcgcgtcc atcgcaaatt tgtagtgagt atagcgggcg
    20701 gtaaattcag gaaacaatgg cggatagcgg aaagtgtagt ttttcggaaa aagataactt
    20761 tatcccgtgt aacccgtttt aagcgggcta cacgggaggc tgcaatttaa acttccagcc
    20821 agttcataat gccgtctgcc gctttgcgac cttcggcaat ggcggtaacg accagatcgg
    20881 aaccacggac gatatcgccg ccagcaaaga ttttcgggtt gctggtctgg aaagcgttgt
    20941 cgttgccttc cggggcgata atgcgcccct gcgaatccag ctcgacgctg tgttttgcca
    21001 gccattccat gctgtgtgga cggaaaccaa acgccatgat caccgcatct gccggaacga
    21061 tatgttctga ccctgccacg atctctgcgc ggcgacggcc tttggcatcc ggttcgccca
    21121 tttctgtgcg caccattttt acgccgctga ctttgccgtt accgttcact tctataccca
    21181 gcggctggac gttgaatttg aactcaacac cttcttcccg cgcgtttttc acttcgcggc
    21241 gggaacccgg catgttctct tcatcacgac gataggcaca ggtaacgtgc tttgccccct
    21301 ggcgtacaga cgtgcgcacg cagtccatcg cagtgtcgcc accgccaagg accaccacgc
    21361 gtttgccttc catgctgacg aacggttcgt cgttggtttc gccaaagccc attaactgtt
    21421 tggtgttggc gataaggaac ggcagcgctg cgtacacgcc atcggcgtct tcgttttcca
    21481 gcccgccgcg cattgactga taagtcccga cgccaaggaa cacggcatcg taatcactca
    21541 gcagatcgtc cagctgtacg tcgcggccca cttcggtatt gagtttgaat tcaataccca
    21601 tgcccgtgaa gatttcacgg cggcgcgtca ttacctcttt ttccagcttg aaggccggaa
    21661 taccgaaggt cagcaagccg ccgatttccg ggtgacggtc gaagacgact gcttttacgc
    21721 cgttgcgcgt caggacatct gcacacgcca gacccgccgg gcctgcgccg ataatcgcca
    21781 cttttttacc ggtctgtttc acgccggaca tatccggacg ccagcccatc tcgaacgctt
    21841 tatcgttgat atagcgctca atgttgccga tggtcactgc gccgaactca tcgttcaggg
    21901 tgcaggaacc ttcgcacaga cggtcttgcg ggcagacacg tccgcacact tccggcaggg
    21961 tattggtctg atgcgacagt tccgccgctt caaaaatacg cccctcgttg gcgagcttca
    22021 gccagttcgg gatgtagttg tgtaccgggc atttccactc gcagtatggg ttgccgcacg
    22081 acaggcagcg gtcagcctgc gctttggcct ggccttcgga aaacggctcg taaatttcaa
    22141 caaactcaat tttgcggatc ttcagcggtt tctttggcgg atcaacgcgc tgcaggtcga
    22201 taaattgata aacattctga ctcattgttg ctacccctta ctgcgcctgc acgcgcaact
    22261 ctgctgcgct acgactacgg tgacccagca gtgcttttac atcactggac ttcggtttaa
    22321 ccagcgcgaa tttcgtggcg aaggttgacc agttcgccag aatctcttca ccgcgctggg
    22381 aaccggtatg ctgcacatgc tcggtgataa gaccacgcag atgctcttca tggatcgcca
    22441 aatcgtcaac gcttaagacc tcgaccagtt ccgggttaac gcgtttgcgg aaatcgccgc
    22501 tttcatcgag aacgtaagcg aagccaccgg tcatgcccgc gccgaagtta acgccggttt
    22561 tacccagaat acagacaata ccgcccgtca tatattcaca gccgttgtcg ccaatgcctt
    22621 ctaccacggt gatagcaccg gagttacgta cgccgaaacg ttcacccgcg cggcctgcgg
    22681 catacagacg acctccggtc gcgccataca ggcaggtgtt gccgataatg cttgcttcat
    22741 ggctgcggaa ggctgagcca accggaggac gaatggcaat taagccgccc gccatgcctt
    22801 taccgacata gtcgttagca tcgccggtca gatacagttc cacgccgccc gcgttccaca
    22861 caccgaagct ctggcctgcg gtgccgttga agtatgcttt gatcggatcg gcagccagcc
    22921 cctgatcgcc gtgcgtctgg gcgatatagc ctgaaagcga cgcgccgaca gaacgatcgg
    22981 tgttgcggat atcgaaccag aaggttttgc tctggcgttc atcgacaaac ggttttgcct
    23041 gttgcagcaa ctgcgcgttc agtaggccgt tatcaaacgg cgggttgttt tcggtgcagt
    23101 agagtgcctt acctgcattt ggctcggcag tttccagcag cttcgacagc gccagtttct
    23161 gctgtttggc ggtgaaaccg tccagctctt tcagcaggtc ggtgcgacca atcagatcca
    23221 ccagacgcgt tacccccagc tgtgccatca gctcgcgggt ttcacgggcg ataaactcaa
    23281 agtaattagt cactttgaac ggcaggccgt gatagtgatt cttacgcagt ttgtcatcct
    23341 gagttgctac acccgtggcg cagttgttca ggtggcaaat tcgcagatat ttacagccaa
    23401 gcgctaccat cgggccagtg ccgaagccga agctttctgc gccgagaatc gccgctttga
    23461 tgatatcaac gccggttttc aggccgccat caacctgcaa acggatcttg tgacgcaagc
    23521 cgttggcaac cagcgcctgt tgggtttcaa caagccccag ctcccacgga cagcctgcgt
    23581 atttcaccga tgaaagcgga cttgcgccgg tgccgccgtc atagcctgcg atggtgatta
    23641 aatcggcata agcttttgcc acgccagtcg cgatggtgcc tacgcccggt tcggaaacca
    23701 gcttcacgga gatcatcgct tttggattga cctgcttgag gtcgaaaatc agctgcgcta
    23761 aatcctcaat agagtagata tcgtggtgcg gcggcgggga gatcagcgtc acgcccggca
    23821 ccgaatagcg cagtttggca atgtaaggag tgactttatc acccggtaac tgaccacctt
    23881 cacccggctt cgcgccctgg gcgactttta tctgaatgac gtcggcattg accaggtacg
    23941 ccggagtaac gccgaagcga ccggacgcca cctgcttgat gcgcgacact ttgttagtgc
    24001 catagcgcgc cgggtcttca ccgccttcgc cggagtttga gttaccgccg atgctgttca
    24061 tcgcttccgc cagcgcctcg tgggcttccg ggcttaacgc gccgatagac atcgcggcgg
    24121 tatcgaagcg tttaaacagt tcgcttgccg gttcaacatc ggcaatgttg actgcgtttt
    24181 cacccggcgt aattgccagc agatcgcgca gcgtggttgc cggacgctca ttaaccagct
    24241 tcgcgtattc ctgatagtcg ctatactcgc cgctctgtac cgcctgttgc agcgtgcgca
    24301 ccacgtccgg gttgtaggcg tggtattcgc cgccgtgcac gtatttcagc aggccgccct
    24361 ggctgatggg cttacgcgcc agccaggcac gtttcgacag attcagcaga tcctgctgga
    24421 agtcttcaaa gctagccccc ccaatgcggc taaccgcccc ctggaagcac aggcccacga
    24481 catcatcgtg cagaccgacc gcttcaaaca gtttcgagca gcggtaagag gcgatggtgg
    24541 agatgcccat tttggacatg attttgtaca agcctttgtt gatgccgtta cgatagttga
    24601 gcatcacggt acgataatct ttggcaatcg cgtgggtgtc taccagacgg cccagcgttt
    24661 cataggcaag gtacggataa atagccgtcg cgccgaaacc cagcaacacg gcgaagtggt
    24721 gcggatcacg ggcgctggcg gtttcgacga tgatgttggc atcgcaacgc aggctttgat
    24781 cgaccagacg ggtctggatc gcgccaaccg ccatcggagc cggaaccggc aggcgatctt
    24841 tagcgatatt ccggtcggag agcaccagca gtacggtgcc gctacgtacc attttttcgg
    24901 ctttgtcgca cagttctttc accgtcgctt cgagcgtggt tttagtgacg tcaaaggtga
    24961 tatccagcgt atctgcgcgg tagtgatcct ctttcatcgt cgtgagctgt ttgaaatcgg
    25021 aatagagcag aatcggcgat ttaaagctta aacggtgcgc ctggccttct gcttcgcaga
    25081 agacgttcat ttcgcggcca atgctggtgg cgagcgacat aacatgcgct tcacgcagcg
    25141 ggtcgattgg cgggttagtc acctgggcaa attgctggcg gaagtagtcg taaataatgc
    25201 gcggctgact ggagagcacg gcgaatgggg tgtcgtcgcc catcgaaccg accgcttcct
    25261 gaccgttttc acccagtacg cggatcaccg aatccagctc ttccgcgctg tagttaaatt
    25321 gtttctggta gctggcaagc gtgtcgtcgt ccagttcgcg gctacccact tcttcatcgg
    25381 gcagatcttc aaacggtacc agacggcgga cgtttttctc catccactct ttataagggt
    25441 ggcggctttt cagatcgtca tcggtttctg ccgagtgcag aatacgccca ctgcgggtgt
    25501 cgatcaccat cagttcgcct ggccccacgc ggcctttttc gaccacttcg tcaggctgat
    25561 aatcccagat accgacttca gaggcgcagg taatgagctt atctttggtg atgacatagc
    25621 gtgccggacg cagaccgtta cggtcgaggt tacaggcggc aaaacgaccg tcagacatca
    25681 cgatgcctgc cgggccatcc cacggctcca tatgcatgga gttaaagtca aagaaggcac
    25741 gcagttccgg gtccatatcc gggttgttct gccaggcggg tggcactaac agacgcatgg
    25801 cgcggatgat atccatcccg cctgccagca gcagttccag catgttatcc atggaactgg
    25861 agtcagagcc ggtttcgttg acgaacggcg cggcgtcgtg caggtcaggg atcagcggcg
    25921 tctggaattt ataggtacgc gcacgcgccc attggcggtt accagtgatg gtgttgattt
    25981 caccgttatg cgcgaggtag cggaacggct gtgccagcgg ccagcgcggt acggtgttag
    26041 tggaaaagcg ctggtggaac aggcaaatgg ccgattccag acgcaggtcc gcaagatcca
    26101 gataaaagcg cggcagatcc gccggcatac acagaccttt atagatgttc accagattcg
    26161 acaggctaca gacgtagaag tctttgtcgg cttcgagacg cttttcaatg cggcggcggg
    26221 cgataaacag acggcgctcc atatcgcgtg gacgccagcc tgccggggcg ttcacaaaaa
    26281 tttgctcaat gcgtggcaga gaggagaggg cgatttcacc cagcacgcct tcgttagtgg
    26341 ggacatcacg ccagcccaca atcgacaatg tttcgcgttg cagctcttct tcaacgatgc
    26401 ggcgtgcggc agcggcgagt tcaggatctt tattcaggaa gagcatcccg acagcgtagt
    26461 tttttgctaa acgccagccg cgctcctgcg caacgatgcg aaaaaagcga tccggttttt
    26521 gtaacagcaa gccgcaaccg tcaccggttt taccatcggc gagaatcgcg ccacggtgct
    26581 gcatgcgggc cagtgcgtgt attgcagtac gcactacctt gtggctaggt tcgccttcta
    26641 tgtgggcgat caggccgaaa ccacagttat ccctctcaag ggatttatcg tacaacatat
    26701 cgtgaacctc ccccaggctc tgcgggaacc ccacttccga accgctgcgc acgggcacag
    26761 aaagagcgtg gcgacggggt ttgcgtgtca tacgcgcacc tcgcattcgc ccccttgttg
    26821 tccttttcgc atcggttaat acggtgaaga ggggatctca attactgcat aaatatgatg
    26881 agcaggctgc tcatccagaa agcttccagc ggatttccaa cttatcggga aagctgatac
    26941 aggtcaaatg gcaagcttat tggtacagaa atgtgcttta gcgcaaaatt ctttattaat
    27001 tgtctgaaat taaagtggat tttctattta aggctaaaag ggcgtcgtta gagaaacagt
    27061 caattcctga ctgtcataaa ggcgtgttaa cccttatcct gccttgaact gacgagaaaa
    27121 ccagcatttt atactgcctt aattggtaat aacgtttcat gctactgttt ttgcctaaaa
    27181 tccataagga gataaaaaaa tctgacacaa gataagcaaa agtgatttat gtgattgtgc
    27241 atgaaatttc ggtaattttg attgattatg cagttgataa aagcaggccg ggacgattga
    27301 tccaggtcat cgccgaattg agctaaaaat ggcaggcttt ggcctctttt caaggcgcgg
    27361 tctatcagat tatgcagtta cagaaattag tcaatatgtt tggtggtgat ctcacccgtc
    27421 gttatgggca aaaggtgcat aagctgacgc tgcacggcgg ttttagctgc cctaaccgtg
    27481 acggtaccat cgggcgtggt ggctgcacat tctgtaatgt ggcctcgttt gccgatgaag
    27541 cgcagcagca tcgttccatt gccgagcaac tggcgcacca ggcgaattta gttaaccgcg
    27601 ctaaacgcta tctggcctac tttcaggcgt ataccagcac ctttgcggaa gttcaggtgc
    27661 tgcgttctat gtatcagcag gcggtgagcc aggccaatat tgtcggtttg tgtgttggta
    27721 cccgcccgga ctgcgtgccg gatgcggtgc tggatctgct ttgcgaatat aaggaccagg
    27781 gttacgaagt gtggctggag ctggggctac aaaccgccca cgacaaaacg ctgcatcgca
    27841 tcaatcgcgg tcatgatttt gcctgttatc agcgcacgac gcagctggcg cgtgagcgtg
    27901 ggctgaaggt gtgttctcac ttaattgtcg gcctgcctgg cgaagggcag gctgaatgtt
    27961 tgcaaacgct ggagcgagtc gttgaaactg gcgtggacgg cataaagctg catccgctgc
    28021 atattgtgaa aggcagcatt atggcgaaag cctgggaagc ggggcgtttg aacggtattg
    28081 aactggaaga ttacacgctc actgccggag aaatgattcg ccatacgcca ccggaagtga
    28141 tctaccaccg catttccgcc agcgcccgtc gcccgacgct gcttgctcct ctgtggtgcg
    28201 aaaatcgctg gacgggaatg gtcgagctgg atcgttatct gaatgaacat ggcgtacagg
    28261 gatcggcgct gggacgtccg tggctacctc caacggcgta ggccattgag gggaattccg
    28321 catttctcac acaatttata acgtaactgt cagaattggg tattattggg gcaggttgtc
    28381 gtgaaggaat tccctaatga agcaaattcg tctgctggcg cagtattatg ttgacctgat
    28441 gatgaagtta ggtctggtgc gcttctcaat gttgctggcg ctggccctcg tcgttcttgc
    28501 cattgtggta caaatggcgg taaccatggt gctgcatggt caggtcgaaa gcattgatgt
    28561 tattcgttct atcttctttg gtttgctgat tacgccgtgg gcggtctact ttctatcggt
    28621 ggtcgtcgag caactggagg agtcacggca acgtctgtca cgactggtgc aaaaactgga
    28681 ggagatgcgc gagcgcgatt tgagcctcaa cgttcagtta aaagataata ttgcccagct
    28741 aaatcaggaa atcgccgttc gtgaaaaagc ggaagcagaa ctgcaggaaa ccttcggcca
    28801 actgaaaatt gaaatcaaag agcgcgaaga gacacaaatt cagctcgagc agcaatcctc
    28861 attcttacgt tccttccttg atgcttcacc cgacctggtt ttttatcgta acgaagataa
    28921 agagttttcc ggctgtaacc gcgcgatgga gctgctgacc ggaaaaagcg aaaaacaact
    28981 ggttcacctg aaacctgctg atgtttactc accggaagcc gccgcaaaag tcattgaaac
    29041 cgatgaaaaa gtgttccgtc ataatgtgtc actgacctat gaacagtggc tggattaccc
    29101 ggacgggcgc aaagcctgct ttgaaatccg taaagtgccg tactacgacc gcgtgggtaa
    29161 acgtcacggt ttgatgggct ttggtcgcga cattaccgag cgtaagcggt atcaggatgc
    29221 gcttgaacgg gccagccgcg acaaaacgac gtttatctcc accatcagtc acgaattgcg
    29281 tacgccgctg aatggtatcg tcggcctgag ccgcattctg ctggataccg aactcaccgc
    29341 cgagcaggaa aaatatctca aaaccatcca tgtttcggcc gtcacgctgg ggaatatctt
    29401 caacgatatt atcgacatgg ataagatgga acggcgcaag gtccagcttg ataatcagcc
    29461 ggttgatttc accagcttcc ttgccgatct gga
//
LOCUS       DAMZFT010000054        29000 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.054, whole genome shotgun sequence.
ACCESSION   DAMZFT010000054 DAMZFT010000000
VERSION     DAMZFT010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 29000)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29000)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29000
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.054"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(<1..797)
                     /gene="lptG"
                     /locus_tag="PN977_002466"
     CDS             complement(<1..797)
                     /gene="lptG"
                     /locus_tag="PN977_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313266.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HDI6042489.1"
                     /translation="MQPFGVLDRYIGKTIFTTIMMTLFMLVSLSGIIKFVDQLKKAGQ
                     GSYDALGAGMYTLLSVPKDVQIFFPMAALLGALLGLGMLAQRSELVVMQASGFTRMQV
                     ALSVMKTAIPLVLLTMAIGEWVAPQGEQMARNYRAQAMYGGSLLSTQQGLWAKDGNNF
                     VYIERVKGDEELGGISIYAFNENRRLQSVRYAATAKFDPEHKVWRLSQVDESDLTNPK
                     QITGSQTVSGTWKTNLTPDKLGVVALDPDALSISGLHNYVKYLKSSG"
     gene            complement(797..1897)
                     /gene="lptF"
                     /locus_tag="PN977_002467"
     CDS             complement(797..1897)
                     /gene="lptF"
                     /locus_tag="PN977_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HDI6042490.1"
                     /translation="MIIIRYLVRETLKSQLAILFILLLIFFCQKLVRILGAAVDGDIP
                     ANLVLSLLGLGVPEMAQLILPLSLFLGLLMTLGKLYTESEITVMHACGLSKAVLVKAA
                     MILAVFTAIVAAVNVMWAGPWSSRHQDEVLAEAKANPGMAALAQGQFQQATNGSSVLF
                     IESVDGSDFKDVFLAQIRPKGNARPSVVVADSGHLTQLRDGSQVVTLNQGTRFEGTAL
                     LRDFRITDFQDYQAIIGHQAVALDPNDTDQMDMRTLWNTDTDRARAELNWRITLVFTV
                     FMMALMVVPLSVVNPRQGRVLSMLPAMLLYLLFFLIQTSLKSNGGKGKLDPTLWMWTV
                     NLIYLALAIVLNLWDTVPVRRLRASFSRKGAV"
     gene            2164..3675
                     /gene="pepA"
                     /locus_tag="PN977_002468"
     CDS             2164..3675
                     /gene="pepA"
                     /locus_tag="PN977_002468"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HDI6042491.1"
                     /translation="MEFSVKSGSPEKQRSACIVVGVFEPRRLSPIAEQLDKISDGYIS
                     ALLRRGELEGKPGQTLLLHHVPNVLSERILLIGCGKERELDERQYKQVIQKTINTLND
                     TGSMEAVCFLTELHVKGRNNYWKVRQAVETAKETLYSFDQLKTNKSEPRRPLRKMVFN
                     VPTRRELTSGERAIQHGLAIAAGIKAAKDLGNMPPNICNAAYLASQARQLADSYSKNV
                     ITRVIGEQQMKELGMHSYLAVGQGSQNESLMSVIEYKGNASEDARPIVLVGKGLTFDS
                     GGISIKPSEGMDEMKYDMCGAAAVYGVMRMVAELQLPINVIGVLAGCENMPGGRAYRP
                     GDVLTTMSGQTVEVLNTDAEGRLVLCDVLTYVERFEPEAVIDVATLTGACVIALGHHI
                     TGLMANHNPLAHELIAASEQSGDRAWRLPLGDEYQEQLESNFADMANIGGRPGGAITA
                     GCFLSRFTRKYNWAHLDIAGTAWRSGKAKGATGRPVALLAQFLLNRAGFNGEE"
     gene            3809..4252
                     /gene="holC"
                     /locus_tag="PN977_002469"
     CDS             3809..4252
                     /gene="holC"
                     /locus_tag="PN977_002469"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004098129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HDI6042492.1"
                     /translation="MKNATFYLLDNDTTVDGLSAVEQLVCEIAAERWRSGKRVLIACE
                     DEKQAYRLDEALWARPAESFVPHNLAGEGPRGGAPVEIAWPQKRSSSPRDILISLRTS
                     FADFATAFTEVVDFVPYEDSLKQLARERYKAYRVAGFNLNTATWK"
     gene            4252..7107
                     /gene="valS"
                     /locus_tag="PN977_002470"
     CDS             4252..7107
                     /gene="valS"
                     /locus_tag="PN977_002470"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000416368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HDI6042493.1"
                     /translation="MEKTYNPQDIEQPLYEHWEKQGYFKPNGDESQESFCIMIPPPNV
                     TGSLHMGHAFQQTIMDTMIRYQRMQGKNTLWQVGTDHAGIATQMVVERKIAAEEGKTR
                     HDYGREAFIDKIWEWKAESGGTITRQMRRLGNSVDWERERFTMDEGLSNAVKEVFVRL
                     YKEDLIYRGKRLVNWDPKLRTAISDLEVENRESKGSMWHIRYPLADGAKTADGKDYLV
                     VATTRPETLLGDTGVAVNPEDPRYKDLIGKYVILPLVNRRIPIVGDEHADMEKGTGCV
                     KITPAHDFNDYEVGKRHALPMINILTFDGDIRESAQVFDTKGNESDVYSSEIPAEFQK
                     LERFAARKAVVAAVDALGLLEEIKPHDLTVPYGDRGGVVIEPMLTDQWYVRADVLAKP
                     AVEAVENGDIQFVPKQYENMYFSWMRDIQDWCISRQLWWGHRIPAWYDEAGNVYVGRN
                     EDEVRKENNLGADVVLRQDEDVLDTWFSSALWTFSTLGWPENTDALRQFHPTSVMVSG
                     FDIIFFWIARMIMMTMHFIKDENGKPQVPFHTVYMTGLIRDDEGQKMSKSKGNVIDPL
                     DMVDGISLPELLEKRTGNMMQPQLADKIRKRTEKQFPNGIEPHGTDALRFTLAALAST
                     GRDINWDMKRLEGYRNFCNKLWNASRFVLMNTEGQDCGFNGGEMTLSLADRWILAEFN
                     QTIKAYREALDSFRFDIAAGILYEFTWNQFCDWYLELTKPVMNGGTEAELRGTRHTLV
                     TVLEGLLRLAHPIIPFITETIWQRVKVLCGITADTIMLQPFPQYDASQVDEAALADTE
                     WLKQAIVAVRNIRAEMNIAPGKPLELLLRGCSADAERRVNENRGFLQTLARLESITVL
                     PADDKGPVSVTKIIDGAELLIPMAGLINKEDELARLAKEVAKIEGEISRIENKLANEG
                     FVARAPEAVIAKEREKLEGYAEAKAKLIEQQAVIAAL"
     gene            complement(7161..8357)
                     /locus_tag="PN977_002471"
     CDS             complement(7161..8357)
                     /locus_tag="PN977_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709944.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF898 domain-containing protein"
                     /protein_id="HDI6042494.1"
                     /translation="MAQVINDMDVPSHSFVFHGTGERYFLICVVNVLLTIITLGIYLP
                     WALMKCKRYLYANMEVNGQRFSYGITGGNVFVSCLVFVFFYFAILMTVSADMPLIGCV
                     LTLSLLVLLIFMAAKGLRYQALMTSLNGVRFSFNCSMKGFWWVTFFLPILMALGMGTV
                     FFISTKMLHANSSSSVIISVVLMTIVGIVSIGIFNGTLYSLVMSFLWSNTSFGIHRFK
                     VKLDTTYCIKYAILAFLALLPFLAVAGYIIFDQILNEYDSSGYANDNIENLQQFMEMQ
                     RKMIIAQLIYYFGIAVSTSYLTVSLRNHFMSNLSLNDGRIRFRSTLTYHGMLYRMCAL
                     VVISGITGGLAYPLLKIWMIDWQAKNTYLLGDLDDLPLINKEEQPDKGFLASISRGVM
                     PSLPFL"
     gene            8550..9053
                     /locus_tag="PN977_002472"
     CDS             8550..9053
                     /locus_tag="PN977_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDI6042495.1"
                     /translation="MNNIAPQSPVMRRLTLQDNPAIARVIRQVSAEYGLTADKGYTVA
                     DPNLDELYQVYSQPGHAYWVVEYEGEVVGGGGIAPLTGSESDICELQKMYFLPAIRGK
                     GLAKKLALMAMEQAREMGFKRCYLETTAFLKEAIALYEHLGFEHIDYALGCTGHVDCE
                     VRMLREL"
     gene            complement(9099..9515)
                     /gene="rraB"
                     /locus_tag="PN977_002473"
     CDS             complement(9099..9515)
                     /gene="rraB"
                     /locus_tag="PN977_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E inhibitor RraB"
                     /protein_id="HDI6042496.1"
                     /translation="MANPEQLEEQREETRLIIEELLEDGSDPDALYTIEHHLSADDLE
                     TLEKAAVEAFKLGYEVTDPEELEVEDGDIVICCDILSECALNADLIDAQVEQLMTLAE
                     KFDVEYDGWGTYFEDPNGEDGDDEDFVDEDDDGVRH"
     gene            9677..10681
                     /gene="argF"
                     /locus_tag="PN977_002474"
     CDS             9677..10681
                     /gene="argF"
                     /locus_tag="PN977_002474"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HDI6042497.1"
                     /translation="MSGFYHKHFLKLLDFTPAELNSLLQLAAKLKADKKSGKEEAKLT
                     GKNIALIFEKDSTRTRCSFEVAAYDQGARVTYLGPSGSQIGHKESIKDTARVLGRMYD
                     GIQYRGYGQEIVETLAEYAGVPVWNGLTNEFHPTQLLADLLTMQEHLPGKTFNEMTLV
                     YAGDARNNMGNSMLEAAALTGLDLRLVAPQACWPEAALVTECRALAQQNGGNITLTED
                     VAKGVEGADFIYTDVWVSMGEAKEKWAERIALLRDYQVNSKMMQLTGNPEVKFLHCLP
                     AFHDDQTTLGKKMAEEFGLHGGMEVTDEVFESAASIVFDQAENRMHTIKAVMVATLSK
                     "
     gene            complement(10753..12406)
                     /locus_tag="PN977_002475"
                     /pseudo
     CDS             complement(10753..12406)
                     /locus_tag="PN977_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036417.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
     gene            complement(12529..12981)
                     /gene="tabA"
                     /locus_tag="PN977_002476"
     CDS             complement(12529..12981)
                     /gene="tabA"
                     /locus_tag="PN977_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin biofilm protein TabA"
                     /protein_id="HDI6042498.1"
                     /translation="MIIGNIHNLQPWLPQELRQAIEHIKANVTAETPKGKHDIEGNRL
                     FYLISEDMTEPYEARRAEYHARYLDIQIVLKGQEGMTFSTQPAGTPDTDWLADKDIAF
                     LPEGVDEKTVILNEGDFVVFYPGEVHKPLCAVGAPAQVRKAVVKMLMA"
     gene            complement(13126..13719)
                     /locus_tag="PN977_002477"
     CDS             complement(13126..13719)
                     /locus_tag="PN977_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709951.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDI6042499.1"
                     /translation="MVTKKQSRVPGRPRRFAPEQAVSAAKVLFHQKGFDAVSVAEVTD
                     YLGINPPSLYAAFGSKAGLFSRVLNEYVGTEAIPLADILRDDRPVGECLVEVLKEAAR
                     RYSQNGGCAGCMVLEGIHSHDPQARDIAVQYYHAAETTIYDYIARRHPQSAQCVTDFM
                     STVMSGLSAKAREGHSIEQLCATAAMAGEAITTLLKE"
     gene            13790..14503
                     /gene="bdcA"
                     /locus_tag="PN977_002478"
     CDS             13790..14503
                     /gene="bdcA"
                     /locus_tag="PN977_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDI6042500.1"
                     /translation="MGAFTGKTVLILGGSRGIGAAIVRRFVTDGANVRFTYAGSKDAA
                     ERLAHETGSTAVFTDSADRDAVIDVVRKSGALDILVVNAGIGVFGDALELNADDIDRL
                     FKINIHAPYHASVEAARQMPEGGRILIIGSVNGDRMPVAGMAAYAASKSALQGMARGL
                     ARDFGPRAITINVVQPGPIDTDANPANGPMRDMLHSFMAIKRHGQPEEVAGMVAWLAG
                     PEASFVTGAMHTIDGAFGA"
     gene            14635..15030
                     /locus_tag="PN977_002479"
     CDS             14635..15030
                     /locus_tag="PN977_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HDI6042501.1"
                     /translation="MVERTAVFPAGRHSLYAEHRYSAAIRSGDLLFVSGQVGSREDGT
                     PEPDFQQQVRLAFDNLHATLAAAGCTFDDIIDVTSFHTDPEKQFEDIMTVKNEIFSAP
                     PYPNWTAVGVTWLAGFDFEIKVIARIPEL"
     gene            15311..15445
                     /gene="pyrL"
                     /locus_tag="PN977_002480"
     CDS             15311..15445
                     /gene="pyrL"
                     /locus_tag="PN977_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyr operon leader peptide"
                     /protein_id="HDI6042502.1"
                     /translation="MVQCVRHFVLPRLKKDAGLPFFFPLITHSQPLNRGAFFCPGVRR
                     "
     gene            15449..16384
                     /gene="pyrB"
                     /locus_tag="PN977_002481"
     CDS             15449..16384
                     /gene="pyrB"
                     /locus_tag="PN977_002481"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HDI6042503.1"
                     /translation="MANPLYQKHIISINDLSRDDLNLVLATAAKLKANPQPELLKHKV
                     IASCFFEASTRTRLSFETSMHRLGASVVGFSDSANTSLGKKGETLADTISVISTYVDA
                     IVMRHPQEGAARLATEFSGNVPVLNAGDGSNQHPTQTLLDLFTIQETQGRLDNLHVAM
                     VGDLKYGRTVHSLTQALAKFDGNRFYFIAPDALAMPQYILDMLDEKGIAWSLHSSIEE
                     VMAEVDILYMTRVQKERLDPSEYANVKAQFVLRASDLHNAKANMKVLHPLPRVDEIAT
                     DVDKTPHAWYFQQAGNGIFARQALLALVLNRDLVL"
     gene            16397..16858
                     /gene="pyrI"
                     /locus_tag="PN977_002482"
     CDS             16397..16858
                     /gene="pyrI"
                     /locus_tag="PN977_002482"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase regulatory
                     subunit"
                     /protein_id="HDI6042504.1"
                     /translation="MTHDNKLQVEAIKRGTVIDHIPAQIGFKLLSLFKLTETDQRITI
                     GLNLPSGEMGRKDLIKIENTFLSEDQVDQLALYAPQATVNRIDNYEVVGKSRPSLPER
                     IDNVLVCPNSNCISHAEPVSSSFAVRKRANDIALKCKYCEKEFSHNVVLAN"
     gene            16931..17317
                     /gene="ridA"
                     /locus_tag="PN977_002483"
     CDS             16931..17317
                     /gene="ridA"
                     /locus_tag="PN977_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminobutanoate/2-iminopropanoate deaminase"
                     /protein_id="HDI6042505.1"
                     /translation="MSKTIATENAPAAIGPYVQGVDLGNMIITSGQIPVNPKTGEVPA
                     DVAAQARQSLDNVKAIVEAAGLKVGDIVKTTVFVKDLNDFATVNATYEAFFTEHNATF
                     PARSCVEVARLPKDVKIEIEAIAVRR"
     gene            complement(17523..20219)
                     /gene="mgtA"
                     /locus_tag="PN977_002484"
     CDS             complement(17523..20219)
                     /gene="mgtA"
                     /locus_tag="PN977_002484"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HDI6042506.1"
                     /translation="MFKEIFTRFIRHLPSRLVHRDPLPGAQQTVNTAVPPSLSAHCLK
                     MAVMPEEELWKTFDTHPEGLNQAEVESAREQHGENKLPAQQPSPWWVHLWVCYRNPFN
                     ILLTILGAISYATEDLFAAAVIALMVAISTLLNFIQEARSTKAADALKAMVSNTATVL
                     RVINDKGENGWLEIPIDQLVPGDIIKLAAGDMIPADLRILQARDLFVAQASLTGESLP
                     VEKAATTRQPEHSNPLECDTLCFMGTTVVSGTAQAMVIATGANTWFGQLAGRVSEQES
                     EPNAFQQGISRVSMLLIRFMLVMAPVVLLINGYTKGDWWEAALFALSVAVGLTPEMLP
                     MIVTSTLARGAVKLSKQKVIVKHLDAIQNFGAMDILCTDKTGTLTQDKIVLENHTDIS
                     GKTSERVLHSAWLNSHYQTGLKNLLDTAVLEGTDEESARSLASRWQKIDEIPFDFERR
                     RMSVVVAENTEHHQLVCKGALQEILNVCSQVRHNGEIVPLDDIMLRKIKRVTDTLNRQ
                     GLRVVAVATKYLPAREGDYQRADESDLILEGYIAFLDPPKETTAPALKALKASGITVK
                     ILTGDSELVAAKVCHEVGLDAGEVVIGSDIETLSDDELANLAQRTTLFARLTPMHKER
                     IVTLLKREGHVVGFMGDGINDAPALRAADIGISVDGAVDIAREAADIILLEKSLMVLE
                     EGVIEGRRTFANMLKYIKMTASSNFGNVFSVLVASAFLPFLPMLPLHLLIQNLLYDVS
                     QVAIPFDNVDDEQIQKPQRWNPADLGRFMIFFGPISSIFDILTFCLMWWVFHANTPET
                     QTLFQSGWFVVGLLSQTLIVHMIRTRRVPFIQSCASWPLMIMTVIVMIVGIALPFSPL
                     ASYLQLQALPLSYFPWLVAILAGYMTLTQLVKGFYSRRYGWQ"
     gene            complement(20360..20413)
                     /gene="mgtL"
                     /locus_tag="PN977_002485"
     CDS             complement(20360..20413)
                     /gene="mgtL"
                     /locus_tag="PN977_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_003933616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mgtA regulatory leader peptide MgtL"
                     /protein_id="HDI6042507.1"
                     /translation="MEPDPTPLPRRRLKLFR"
     gene            20598..21545
                     /gene="treR"
                     /locus_tag="PN977_002486"
     CDS             20598..21545
                     /gene="treR"
                     /locus_tag="PN977_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator TreR"
                     /protein_id="HDI6042508.1"
                     /translation="MQNRLTIKDIARLSGVGKSTVSRVLNNESGVSQRTRERVEAVMN
                     QHGFSPSRSARAMRGQSDKVVAIIVTRLDSLSENLAVQTMLPAFYEQGYDPIMMESQF
                     SPQLVAEHLGGLKRRNIDGVVLFGFTGITEEMLAHWQSSLVLLARDAKGFASVCYDDE
                     GAIKILMQRLYDQGHRNISYLGVPHSDVTTGKRRHEAYLAFCKAHKLHPVAALPGLAM
                     KQGYENVAKVITPETTALLCATDTLALGASKYLQEQRIDTLQLASVGNTPLMKFLHPE
                     IVTVDPGYAEAGRQAACQLIAQVTGRSEPQQIIIPATLS"
     gene            21664..23085
                     /gene="treB"
                     /locus_tag="PN977_002487"
     CDS             21664..23085
                     /gene="treB"
                     /locus_tag="PN977_002487"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS trehalose transporter subunit IIBC"
                     /protein_id="HDI6042509.1"
                     /translation="MMSKINQTDIDRLIELVGGRGNIATVSHCITRLRFVLNQPANAR
                     PKEIEQLPMVKGCFTNAGQFQVVIGTNVGDYYQALIASTGQAQVDKEQVKKAARQNMK
                     WHEQLISHFAEIFFPLLPALISGGLILGFRNVIGDLPMSNGQTLAQMYPSLQTIYDFL
                     WLIGEAIFFYLPVGICWSAVKKMGGTPILGIVLGVTLVSPQLMNAYLLGQQLPEVWDF
                     GMFSIAKVGYQAQVIPALLAGLALGVIETRLKRIVPDYLYLVVVPVCSLILAVFLAHA
                     LIGPFGRMIGDGVAFAVRHLMTGSFAPIGAALFGFLYAPLVITGVHQTTLAIDLQMIQ
                     SMGGTPVWPLIALSNIAQGSAVIGIIISSRKHNEREISVPAAISAWLGVTEPAMYGIN
                     LKYRFPMLCAMIGSGLAGLLCGLNGVMANGIGVGGLPGILSIQPSYWQVFALAMAIAI
                     IIPIVLTSFIYQRKYRLGTLDIV"
     gene            23135..24790
                     /gene="treC"
                     /locus_tag="PN977_002488"
     CDS             23135..24790
                     /gene="treC"
                     /locus_tag="PN977_002488"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HDI6042510.1"
                     /translation="MTHLPHWWQNGVIYQIYPKSFQDTTGSGTGDLRGVIQRLDYLHK
                     LGVDAIWLTPFYVSPQVDNGYDVANYTAIDPTYGTLDDFDELVTQAKSRGIRIILDMV
                     FNHTSTQHAWFREALNKESPYRQFYIWRDGEPETPPNNWRSKFGGSAWRWHAESEQYY
                     LHLFAPEQADLNWENPAVRAELKKVCEFWANRGVDGLRLDVVNLISKDPRYPEDLDGD
                     GRRFYTDGPRAHEFLHEMNRDVFTPHGLMTVGEMSSTSLEHCQRYAALTGSELSMTFN
                     FHHLKVDYPGGEKWTLAKPDFVALKTLFRHWQQGMHNVAWNALFWCNHDQPRIVSRFG
                     DEGEYRVPAAKMLAMVLHGMQGTPYIYQGEEIGMTNPHFTRITDYRDVESLNMFAELR
                     NDGRDADELLAILASKSRDNSRTPMQWTNGDNAGFTAGEPWIGLGDNYQEINVEAALA
                     DESSVFYTYQKLIALRKQEAVLTWGDYQDLLPNSPVLWCYRREWKGQTLLVIANLSRE
                     TQPWQPGKMLGNWQLVMHNYEEASPQPCAMTLRPFEAVWWLQK"
     gene            25184..27322
                     /gene="nrdD"
                     /locus_tag="PN977_002489"
     CDS             25184..27322
                     /gene="nrdD"
                     /locus_tag="PN977_002489"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HDI6042511.1"
                     /translation="MTPHVMKRDGCKVPFKSERIKEAILRAAKAAEVDDADYCATVAA
                     VVSEQMQGRNQVDINEIQTAVENQLMSGPYKQLARAYIEYRHDRDIEREKRGRLNQEI
                     RGLVEQTNSSLLNENANKDSKVIPTQRDLLAGIVAKHYARQHLLPRDVVQAHERGDIH
                     YHDLDYSPFFPMFNCMLIDLKGMLTQGFKMGNAEIEPPKSISTATAVTAQIIAQVASH
                     IYGGTTINRIDEVLAPFVTASYNKHRKTAEEWNIPDAEGYANSRTIKECYDAFQSLEY
                     EVNTLHTANGQTPFVTFGFGLGTSWESRLIQESILRNRIAGLGKNRKTAVFPKLVFAI
                     RDGLNHKKGDPNYDIKQLALECASKRMYPDILNYDQVVKVTGSFKTPMGCRSFLGVWE
                     NENGEQIHDGRNNLGVISLNLPRIALEAKGDEATFWKLLDERLVLARKALMTRIARLE
                     GVKARVAPILYMEGACGVRLNADDDVSEIFKNGRASISLGYIGIHETINALFGGEHVY
                     DNEQLRAKGIAIVERLRQAVDQWKEETGYGFSLYSTPSENLCDRFCRLDTAEFGVVPG
                     VTDKGYYTNSFHLDVEKKVNPYDKIDFEAPYPPLANGGFICYGEYPNIQHNLKALEDV
                     WDYSYQHVPYYGTNTPIDECYECGFTGEFECTSKGFTCPKCGNHDASRVSVTRRVCGY
                     LGSPDARPFNAGKQEEVKRRVKHLGNGQIG"
     gene            27507..27971
                     /gene="nrdG"
                     /locus_tag="PN977_002490"
     CDS             27507..27971
                     /gene="nrdG"
                     /locus_tag="PN977_002490"
                     /EC_number="1.97.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate
                     reductase-activating protein"
                     /protein_id="HDI6042512.1"
                     /translation="MNFHQYYPVDIVNGPGTRCTLFVSGCVHECPGCYNKSTWRVNSG
                     QPFTKAMEDQIINDLNDTRIKRQGISLSGGDPLHPQNVPDILKLVQRIRAECPGKDIW
                     VWTGYKLDELNAAQMQVVDLINVLVDGKFMQDLKDPSLIWRGSSNQVVHHLR"
     gene            complement(27972..28262)
                     /locus_tag="PN977_002491"
     CDS             complement(27972..28262)
                     /locus_tag="PN977_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HDI6042513.1"
                     /translation="MNYELAFDPRALKEWQKLGVTVREQFKKKLEDVLKRPRNPSAKL
                     RDLPDCYKIKLRTLGYRLVYSVNDKELLVLVIAIGKRENSAVYEDATKRLDE"
     gene            complement(28252..28494)
                     /locus_tag="PN977_002492"
     CDS             complement(28252..28494)
                     /locus_tag="PN977_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HDI6042514.1"
                     /translation="MATLNVRLDDKLKNEAYAVLEKLNITPTEAVRLLFQYVAENGRM
                     PVKTLTVSDSEEALLRTVRERLANPQEAIKVRLDEL"
     gene            complement(28686..>29000)
                     /gene="cybC"
                     /locus_tag="PN977_002493"
     CDS             complement(28686..>29000)
                     /gene="cybC"
                     /locus_tag="PN977_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b562"
                     /protein_id="HDI6042515.1"
                     /translation="LEDNMETLNDNLKVVEKADNAVQVKDALTKMRAAALDAQKATPP
                     KLEGKSPDSPEMKDFRHGFDILVGQIDDALKLANEGKVKEAQAAAEQLKTTRNAYHQK
                     YR"
BASE COUNT         7450 a         7381 c         7117 g         7052 t
ORIGIN      
        1 tgaccgctcg acttcagata cttcacatag ttgtgcaaac cactgatgga gagtgcatcc
       61 gggtccagcg ccaccacgcc cagtttgtct ggcgtgaggt tggttttcca ggtgccgctc
      121 accgtctgcg aaccggtgat ctgtttcgga ttggtcagat cagattcatc aacctgcgac
      181 agacgccaga ctttatgttc cgggtcaaac ttcgcggtag cggcataacg tacggattgc
      241 agacgacgat tctcgttaaa ggcataaata ctgatgccac ctaactcttc gtcaccttta
      301 acccgctcaa tgtagacgaa gttgttgcca tctttcgccc ataagccttg ctgagtggag
      361 agcaacgagc cgccgtacat cgcctgcgca cggtagttac gcgccatctg ctcgccctgc
      421 ggcgcgaccc attcaccaat cgccatcgtc agcaagacca gcggaatggc ggttttcatc
      481 accgacagcg ccacctgcat acgggtaaaa ccagaagcct gcatgactac cagttcgctg
      541 cgctgcgcca gcatcccaag accaagcaac gccccaagca gagccgccat cgggaagaag
      601 atttgcacat ctttcggcac gctcagcaag gtatacattc ctgcgcctaa cgcgtcgtaa
      661 ctcccctgcc cggctttttt cagctgatcg acaaacttga taatgcccga cagcgacacc
      721 agcatgaaca gcgtcatcat gatggtggtg aaaatagttt taccgatata gcggtcaagt
      781 acgccaaaag gttgcatcac accgctcctt tacgcgaaaa actggcgcgc aggcggcgga
      841 ccggcacggt gtcccaaagg ttgagaacaa tcgccaaagc cagataaatc aggttaacgg
      901 tccacatcca cagcgtcggg tccagcttac ctttaccgcc gttcgatttc agggaggtct
      961 ggatcaggaa gaacaacaga tacagcagca tggctggcag catcgatagt acgcgtccct
     1021 gacgcgggtt aaccacgctc agcggtacga ccataagtgc catcataaac acggtgaaca
     1081 ccaacgtgat acgccagttc agttctgcgc gagcacgatc ggtgtcagtg ttccacaatg
     1141 tgcgcatgtc catctggtcg gtatcgttcg ggtcgagcgc caccgcctgg tgaccaatga
     1201 tcgcctgata atcctggaaa tccgtaatgc ggaaatcacg taacagtgca gtgccttcga
     1261 agcgcgttcc ctggttgaga gtgacgacct gggagccgtc gcgcagctgg gttaaatgtc
     1321 cggaatcggc caccaccaca gaaggtcgtg cattaccttt tggtcgaatt tgcgcgagga
     1381 acacatcttt gaaatcgctg ccgtcaacgc tttcgatgaa cagcaccgag ctgccattag
     1441 tcgcttgctg gaattgccct tgcgcaagcg ccgccatgcc agggttcgct ttcgcttctg
     1501 ctaacacttc atcctgatga cgcgatgacc acggtcccgc ccacatcacg ttaaccgccg
     1561 cgacgattgc cgtgaatacc gcaaggatca ttgccgcttt caccagcacc gctttgctca
     1621 ggccgcaggc atgcattacc gtaatttcac tttcggtata cagtttgccc agcgtcatca
     1681 gcagcccgag gaacaggctt aatggcagga taagctgcgc catttccggc acgcccaacc
     1741 cgagaaggga gagcaccaga ttcgccggaa tatcgccgtc aaccgctgcg ccgaggatcc
     1801 tcactaactt ttgacagaag aagatcaaaa gcaagatgaa gagtatcgcc agctggcttt
     1861 tgagcgtctc ccgcaccaga tatcttatga ttatcacttt aaatacgccc gtaaaaactc
     1921 gtcttttgca ggattttagc ttgtttcatg gcttaaacgt catttattct cttgagtcgt
     1981 cgaaatcgtc gctaagataa ttatactcaa cggattcacc tctcagattt tgttctgacg
     2041 tgccaatgcc gtaataacgt taagattaac acgaagtcat cgcaacagcg gacatgagtt
     2101 acgaaagctt gcaattctat ctgtagccac cgccgttgtc tttaagattc aggagcgtag
     2161 tgcatggagt ttagtgtaaa aagcggtagc ccggagaaac agcggagtgc ctgcatcgtc
     2221 gtgggcgtct tcgaaccacg tcgcctttct ccgattgcag aacagctcga taaaatcagc
     2281 gatgggtaca tcagcgccct gctacgtcgg ggcgaactgg aaggaaaacc ggggcagaca
     2341 ctgttgctgc accatgttcc gaatgtactt tccgagcgaa ttctccttat tggttgcggc
     2401 aaagaacgtg agctggatga acgtcagtac aagcaggtta ttcagaaaac cattaatacg
     2461 ctgaatgata ctggctcaat ggaagcggtc tgctttctga ctgaactgca cgttaaaggc
     2521 cgtaacaact actggaaagt gcgtcaggct gtcgagactg caaaagagac gctctacagt
     2581 ttcgatcagc tgaaaacgaa caagagcgaa ccgcgtcgtc cgctgcgtaa aatggtgttc
     2641 aacgtgccga cccgccgtga actgaccagc ggtgagcgcg cgatccagca cggtctggcg
     2701 attgccgccg ggattaaagc agcaaaagat ctcggcaata tgccgccgaa tatctgtaac
     2761 gccgcttacc tcgcttcaca agcgcgccag ctggctgaca gctacagcaa gaatgtcatc
     2821 acccgcgtta tcggcgaaca gcagatgaaa gagctgggga tgcattctta tctggcggtc
     2881 ggtcagggtt cgcagaacga atcgctgatg tcggtgattg agtacaaagg caacgcgtcg
     2941 gaagatgctc gcccaatcgt gctggtgggt aaaggtttaa ccttcgactc cggcggtatc
     3001 tccatcaagc cttcagaagg catggatgag atgaagtacg atatgtgcgg cgcggcggcg
     3061 gtttacggcg tgatgcgtat ggtcgcggag ctgcaactgc cgattaacgt tatcggcgtg
     3121 ctggcaggct gcgaaaacat gcctggcggg cgtgcctatc gtccgggcga tgtgttaacc
     3181 accatgtccg gtcaaaccgt tgaagtgctg aacaccgatg ccgaaggccg cctggtactg
     3241 tgcgacgtgt taacttacgt tgaacgtttt gagccggaag cggtgattga tgtggcgacg
     3301 ctgaccggtg cctgcgtgat cgcgctgggt catcatatta ctggtctgat ggcgaaccat
     3361 aatccgctgg cccatgaact gattgccgcg tctgaacaat ccggtgaccg cgcatggcgc
     3421 ttaccgctgg gtgacgagta tcaggaacaa ctggagtcca attttgccga tatggcgaac
     3481 attggcggtc gtcctggtgg ggcgattacc gcaggttgct tcctgtcacg ctttacccgt
     3541 aagtacaact gggcgcacct ggatattgca ggaaccgcct ggcgttctgg taaagcaaaa
     3601 ggcgcaaccg gtcgtccggt agcgttgctg gcacagttcc tgctgaatcg cgctgggttt
     3661 aacggcgaag agtaagtttg cgttttaccc tcaccctaac cctctcccta agggaggggg
     3721 aaccgatcga gcgcaataga aattcattgc tcatctttca gggtgaggaa tgcagcaata
     3781 tttaaatcca ccacaagaag ccccatatat gaaaaacgca acgttctacc ttctggacaa
     3841 tgacaccacc gtcgatggct taagcgccgt tgagcaactg gtgtgtgaaa ttgccgcaga
     3901 acgttggcgc agcggtaagc gcgtactcat cgcctgtgaa gatgaaaagc aggcttaccg
     3961 gctggatgaa gccctgtggg cgcgtccggc agaaagcttt gtcccgcata atttagcggg
     4021 agaaggaccg cgcggcggtg cgccggtgga gatcgcctgg ccgcaaaaac gcagcagcag
     4081 cccgcgcgat atattgatta gtctgcgaac aagctttgca gattttgcca ccgctttcac
     4141 agaagtggta gacttcgttc cttatgaaga ttctctgaaa caactggcgc gcgaacgcta
     4201 taaagcctac cgcgtggctg gtttcaacct gaatacggca acctggaaat aatggaaaag
     4261 acatataacc cacaagatat cgaacagccg ctttacgagc actgggaaaa gcagggctac
     4321 tttaagccta atggcgatga aagccaggaa agtttctgca tcatgatccc gccgccgaac
     4381 gtcaccggca gtttgcatat gggtcacgcc ttccagcaaa ccatcatgga taccatgatc
     4441 cgctatcagc gtatgcaggg taaaaacacc ctgtggcagg tcggtactga ccacgccggg
     4501 atcgctaccc agatggtcgt tgagcgcaag attgccgcag aagaaggtaa aacccgtcac
     4561 gattacggcc gcgaagcttt catcgacaaa atctgggaat ggaaagcgga atccggcggc
     4621 accattaccc gtcagatgcg tcgtctcggc aactccgtgg actgggagcg tgaacgcttc
     4681 acgatggacg aaggcctgtc caatgcagtg aaagaagttt tcgttcgtct gtataaagaa
     4741 gacctgattt accgtggcaa acgcctggta aactgggacc cgaaactgcg caccgctatc
     4801 tccgacctgg aagtggaaaa ccgcgaatcg aaaggttcga tgtggcacat ccgctatccg
     4861 ctggctgacg gcgcgaaaac cgcagacggt aaagattatc tggtggttgc gactacccgt
     4921 ccggaaaccc tgctgggcga taccggcgtg gccgttaacc cggaagatcc gcgttataaa
     4981 gatctgattg gcaaatatgt cattctgccg ctggttaacc gtcgtattcc gatcgttggc
     5041 gacgaacacg ccgacatgga aaaaggcact ggctgcgtga agatcacccc agcgcacgac
     5101 tttaacgact atgaagtcgg taaacgtcac gccctgccga tgatcaacat cctgaccttt
     5161 gacggtgata tccgtgaaag cgcccaggtg ttcgatacca aaggtaacga atctgacgtt
     5221 tattccagcg aaatcccggc agagttccag aaactcgagc gttttgctgc acgtaaagcc
     5281 gtcgttgctg cggttgacgc gctcggcctg ctggaagaaa ttaaaccgca cgacctgacc
     5341 gttccttacg gcgaccgtgg cggcgtggtt atcgaaccga tgctgaccga ccagtggtac
     5401 gtgcgtgccg atgtactggc gaaaccggca gttgaagcgg ttgagaacgg cgacattcag
     5461 ttcgtaccga agcagtacga aaacatgtac ttctcctgga tgcgcgatat tcaggactgg
     5521 tgtatctctc gtcagctatg gtggggtcac cgtatcccgg catggtatga cgaagcgggt
     5581 aacgtttatg ttggccgcaa cgaagacgaa gtgcgtaaag aaaataacct cggtgctgat
     5641 gttgtcctgc gtcaggacga agacgttctc gacacctggt tctcttctgc gctgtggacc
     5701 ttctctaccc ttggctggcc ggaaaatacc gacgccctgc gtcagttcca cccaaccagc
     5761 gtgatggtat ctggtttcga catcatcttc ttctggattg cccgcatgat catgatgacc
     5821 atgcacttca tcaaagatga aaatggcaaa ccgcaggtgc cgttccacac tgtttacatg
     5881 accggtctga ttcgtgacga cgaaggccag aagatgtcca aatccaaggg taacgttatc
     5941 gacccgctgg atatggttga cggtatttcg ctgccagaac tgctggaaaa acgtaccggc
     6001 aatatgatgc agccgcagct ggcggacaaa atccgtaagc gcaccgagaa acagttcccg
     6061 aacggcattg agccgcacgg caccgatgcc ctgcgcttca ccctggcggc gctggcttct
     6121 accggtcgtg acatcaactg ggatatgaag cgtctggaag gctaccgtaa cttctgtaac
     6181 aagctgtgga acgccagccg ctttgtgctg atgaacacag aaggtcagga ttgcggcttc
     6241 aacggcggtg aaatgacgct gtcgctggcg gaccgctgga ttctggcgga gttcaaccag
     6301 accatcaaag cgtaccgcga agcgctggac agcttccgtt tcgatattgc cgcaggcatt
     6361 ctgtatgaat tcacctggaa ccagttctgt gactggtatc tggagctgac caagccggta
     6421 atgaacggtg gcaccgaagc cgaactgcgc ggtactcgcc atacgctggt gactgtactg
     6481 gaaggtctgc tgcgcctcgc gcatccgatc attccgttca tcaccgaaac catctggcag
     6541 cgtgtgaaag tactttgcgg tatcactgcc gacaccatca tgctgcaacc gttcccgcag
     6601 tacgatgcgt ctcaggtcga tgaagccgca ctggccgaca ccgagtggct gaagcaggcg
     6661 atcgttgcgg tacgtaacat ccgtgcagaa atgaacatcg caccgggtaa accgctggag
     6721 ctgctgctgc gtggttgcag cgcggatgca gaacgtcgcg taaatgaaaa ccgtggcttc
     6781 ctgcaaacgc tggcgcgtct ggaaagtatc accgtgctgc ctgccgatga caaaggtccg
     6841 gtttccgtta ccaagatcat cgacggcgca gagctgctga tcccgatggc tggcctcatc
     6901 aacaaagaag atgagctggc gcgtctggcg aaagaagtgg cgaagattga aggtgaaatc
     6961 agccgtatcg agaacaaact ggcgaacgaa ggctttgtcg cccgcgcacc ggaagcggtc
     7021 atcgcgaaag agcgtgagaa gctggaaggc tatgcagaag cgaaagcgaa gctgattgaa
     7081 cagcaggctg ttatcgccgc gctgtaatca acaccgtgat gaaaacgaag gccggagcat
     7141 gcgccggcct tttttatctc ttacagaaat ggtaaagaag gcataactcc ccgtgaaata
     7201 ctggctaaga agcctttatc tggttgttct tctttattga ttaaaggaag gtcatccaaa
     7261 tcgcccagca aatacgtatt ttttgcctgc cagtcaatca tccatatttt cagcagtggg
     7321 taagccagac cacccgtaat cccggatatc accaccaacg cacacatgcg ataaagcata
     7381 ccgtggtacg ttaaagttga gcgaaaacga atacgcccat cattcagtga caggttgctc
     7441 ataaaatgat ttcgcaacga caccgttaaa taactggtgc taacagcaat cccaaaataa
     7501 tagattaact gcgcgattat cattttacgt tgcatttcca taaattgctg taaattctca
     7561 atattatcat ttgcataccc agaactatca tactcattta atatttgatc gaagataata
     7621 taaccagcaa cagcgagaaa aggtaataaa gctaaaaatg cgagaatggc atattttata
     7681 caatatgtag tatctaattt caccttgaaa cggtgtatac cgaaactggt attactccag
     7741 agaaaactca ttaccagact atataaagtg ccattaaaaa taccaatgga aacaatacca
     7801 actattgtca tcagaaccac agatataata acgctacttg aactattggc atgtagcatc
     7861 tttgtcgaga taaagaaaac agtccccatc ccaagggcca ttaaaatcgg caagaaaaag
     7921 gtcacccacc agaacccttt catagagcaa ttaaaactaa atcttacgcc gttgagactg
     7981 gtcatcaagg cctgataacg cagtcctttt gctgccataa atataagcaa aaccaacagt
     8041 gacaaagtca aaacacagcc aataagcggc atatctgctg acactgtcat taagattgcg
     8101 aaatagaaaa aaacaaaaac aagacaacta acaaaaacat tcccaccggt aatcccataa
     8161 gaaaatcgtt gtccgttaac ttccatatta gcatagagat aacgcttaca tttcattaat
     8221 gcccatggta aatagatacc tagcgttata atcgttaaca acacattcac cacacaaata
     8281 agaaaatatc tctcacctgt accatgaaaa acaaacgaat gggacggaac atccatatca
     8341 ttaataactt gagccataaa aattccctga attaaaaaat ccattgatat aatttatata
     8401 tacagacagt aaaatgttct gccacgttac aatactctta tcagtaatag gctggcaaaa
     8461 actttattgc tttataagat gcttgcgcac gtaagaagag agtggtatta aacaaatgta
     8521 taattacttt atttttattg ggttaaacta tgaataacat tgcgccgcaa tcacctgtaa
     8581 tgcgtcgcct gacgctgcag gataatcctg ctatcgcccg cgtcattcgt caggtatccg
     8641 ccgaatacgg tcttaccgct gataaaggct acaccgtcgc cgatccgaat cttgacgagc
     8701 tgtatcaagt gtatagtcag cctggccatg catattgggt ggttgagtac gagggtgaag
     8761 tggtcggcgg cggtgggatt gcgccattaa ccgggagtga gtcggacatt tgcgaactgc
     8821 aaaagatgta ttttcttccg gctattcgcg gtaaagggct agcgaaaaaa ctggccttaa
     8881 tggcgatgga gcaggcacga gagatgggct tcaaacgctg ctatctggaa acgaccgctt
     8941 ttttaaagga agccattgcg ctttatgagc atttgggctt tgagcatatc gactatgcgc
     9001 ttggctgcac gggccatgtc gattgtgaag tgcggatgct acgcgaactc taaaaaaatg
     9061 cccgctagta tgtgctatcg ggcattttaa tcaattaatt agtggcgaac cccgtcatcg
     9121 tcttcatcga caaaatcttc atcgtcgcca tcttcgccgt taggatcttc aaagtaagtg
     9181 ccccaaccgt cgtactcaac gtcaaatttc tccgccagcg tcatcagttg ttcaacctgg
     9241 gcatcgatca gatcggcatt caacgcgcac tcgctgagga tgtcgcagca aatcacgata
     9301 tcaccatctt caacttccag ctcttctgga tcggtcactt cgtaaccgag tttaaacgct
     9361 tcaactgccg ctttttccag agtttccaga tcgtctgcgg aaagatggtg ttcgatggtg
     9421 tacagcgcgt ccggatcgct gccatcttcc agtaattctt caataatcaa acgtgtttct
     9481 tcacgctgtt cttccagttg ttccgggttt gccatggctc attcctctta atgtcctgcc
     9541 gatactctta ttgtcacaca ccactgacat tgcctccacc tttgtgataa agatttatgc
     9601 tttagacttg caaatgaata ataattcata taaattgaat tttaattcat taaggcgtta
     9661 gccacaggag ggatctatgt ccgggtttta tcataagcat ttcctgaaat tactcgattt
     9721 cacgccagct gaactcaaca gcctgctgca gttagccgcg aagctgaaag ccgataagaa
     9781 aagcggtaaa gaagaagcca aactcactgg taaaaacatc gcgctcatct tcgaaaaaga
     9841 ctcgacccgt acccgatgct ctttcgaagt tgccgcatat gaccagggtg ctcgtgttac
     9901 ttatctcggc ccaagcggca gccaaattgg tcataaagag tcgattaaag acactgcccg
     9961 cgtgcttggt cgcatgtatg acggtattca gtatcgcggc tatggtcagg agattgtcga
    10021 aacactggcg gaatacgctg gcgtgccggt atggaacggc ctgaccaatg agtttcaccc
    10081 cacgcagctg ctggcggatc tcctcaccat gcaggagcat ttgcccggca aaacgttcaa
    10141 cgaaatgacg ctggtctatg caggtgacgc acgtaacaac atgggcaatt cgatgctcga
    10201 agctgcagcg cttaccggtc tggatttgcg tctggtcgcg ccacaggcat gctggccgga
    10261 agctgcgctg gttacggaat gccgcgccct ggcacaacaa aatggcggga atattacgct
    10321 gacggaagat gtagctaaag gagttgaagg tgctgacttt atctataccg atgtatgggt
    10381 gtcgatgggt gaagcaaaag agaaatgggc tgaacgcatt gcattgctgc gtgattatca
    10441 agtgaacagc aagatgatgc agttgaccgg taacccggag gtcaaattcc tccactgcct
    10501 gcccgcgttt catgacgacc aaacgacgct tggcaaaaaa atggcggaag agtttggcct
    10561 acatggcgga atggaagtga ctgatgaggt cttcgaatct gccgccagca ttgtatttga
    10621 tcaggcggaa aaccgcatgc ataccatcaa agcggtgatg gtcgcgacgc tcagtaaata
    10681 attgatatcg ctataaagat gccgggaaat acaaaaacat cccggcgtca cattatttta
    10741 tgcagtgata gttcaattat tccaccccaa tttttttatt gctatacgta aatcttccgg
    10801 aggatttaat tttcctaact gttcttcatt aatatatcca tatatattga ttttgccatt
    10861 ccggaggatt ttcatcattc ttgttctaat ttttaattca ttacccatct tttcttcaaa
    10921 aatcttaacg acataacccg gaagtttatt tacaatttct tttgtttttt cttcatcacc
    10981 ataaacatat cctaactcat taagatacag ctgttcgccc tcataacact taagtaattg
    11041 atagaagtac cacttagcaa aatcaggttg cttcatgaaa aaatccttca aaaaaaatat
    11101 catttactaa aatgctgaca ggcacttcat taaatagtaa cttaacatca tcaaataacc
    11161 ttagtattaa gcgtgatttt tcttgattgc ttaagctaaa atcattgaca tatgcaacca
    11221 acttatccca aatcaattcc tgtaatttac ggctattttt atagggttct ttatcgagat
    11281 tgaaaaataa ggtctcagct attttaattt tagattgatc gcagctaaaa tctttagatt
    11341 gatcgcggct aaaatcaata tgatttatca cagagcatgc atgctttatc tttgctgaat
    11401 catcaaaata ttgcatgctt ttattaaaac accaatccag tattcttaca tcattaatat
    11461 atggggattt taaaaaagtc tccagaatcg aaacgatgac cggtgcaagg ttaacttttg
    11521 catcatctaa ggaatcaata agaatatgag acaggttaac tttgatgtca ttaatttcat
    11581 tgtcagtagc ttttttatta ttaataagat aattatttaa tacgttaagg tcatctattt
    11641 ctttcagtat ccctttttta cccagcaagt atgaaatcga ttcattacat acataaggca
    11701 ttaatagcag atgttctttc cttattgcta ataatgcatt attcaatggt ttttctaata
    11761 aatccgtaat cgtagaatca actgaacctt tacaactcac ctgcgagtta tttaagtcat
    11821 gagaattatt taactcctgc ggaatattta atagacgagc tatttccttg ttagcttcgc
    11881 gttggtaaca cgccagaatc ttgttagtaa tcaggtgtaa tgaactctca tcttttcttt
    11941 caaaggactt tccttcataa cgattagtcg aaccatcagg ttgttgaaat ataatagccg
    12001 tatgtccttg acgagtcccc atagtagtga tagtggaaag tgtaatttct atttccacct
    12061 tagataaatc cgctcgatcc ccttcaggat tattcataac aaacttcatt aactcactgc
    12121 catcattatt tttgatataa gatgaggact caatatgagc catcatattt cttcccgctt
    12181 cgttggagcg tttgctaaaa tccacataac ccagggtaaa tatttttacc aaaaactcca
    12241 aaaatgtctt aggatcaagg atatttcttg tattatcctg cccctcggca ttacgggcaa
    12301 aattattcat tccaacacgc caggtattta aaacttcact tttttgttta aaattgtcgg
    12361 ctaatgtaat gtgttgaaaa taattcaaat ctgatatttt gctcatgacg aactccttga
    12421 gttaataaga acgtctaagc attacaaatt ttactcacaa aaaaatatca aaaaacgctc
    12481 ttttcacaaa ctcgccgtaa acctggagtt atttacggcg aaaagtcatc acgccatcag
    12541 catcttcact actgctttgc gaacctgggc tggcgcgccc actgcgcaca gcggtttatg
    12601 cacttccccc ggataaaaca caacaaaatc accttcatta agaataactg ttttctcatc
    12661 aacgccttcc ggcaaaaatg cgatgtcttt gtctgctaac cagtcggtat ccggggtgcc
    12721 tgcaggttgc gtgctgaagg tcatgccttc ctgacctttt aacacaatct gaatgtcgag
    12781 atagcgggca tggtactccg cacggcgagc ttcgtacggc tcggtcatat cttccgagat
    12841 aagataaaac agacgattgc cttcgatatc gtgcttgccc ttgggcgttt ctgccgtaac
    12901 gtttgctttg atatgctcaa tcgcctggcg taactcttgg ggtagccacg gctgaagatt
    12961 atgaatattt ccgatgatca tcttttccac tccttaatta aaacaatgtt tcatttctat
    13021 cgttatacga aaagatgatc aattccgcca gccttaacgc gtgaattatt gtcttatcac
    13081 aacttatcgg caggcgcgtt cccgcctttc ggatcaaggc cagcatcact ccttgagaag
    13141 agttgttatc gcttcccccg ccatggcagc tgttgcacag agttgttcga tggagtgccc
    13201 ctcccgtgcc ttcgcagaaa gccctgacat cacggtactc ataaaatcag tcacacattg
    13261 tgcgctttgt ggatgccgcc tggcgatata gtcataaatg gtcgtttctg cggcgtgata
    13321 atattgaacg gcaatatcac gcgcttgtgg atcatgacta tgaatacctt caagaaccat
    13381 acagccagcg cagccgccgt tttggctata tctgcgcgcc gcttctttta atacctcaac
    13441 caggcactcg cctaccggac gatcatcacg aagaatatcg gcaagcggaa tagcttccgt
    13501 accgacatat tcattgagta cacggctaaa taacccagcc ttactgccaa aagccgcgta
    13561 gaggctcggg gggttaatac caagataatc agtaacttca gcaacactga cagcatcgaa
    13621 acctttttgg tgaaaaagca cttttgccgc agagactgcc tgctcaggag cgaaacgtct
    13681 gggacgacct ggaacgcgag attgtttttt agtgaccatt acaaaacttg ttgacagaaa
    13741 gttaaaacag ttttgtaatg catgttacat aataaatcaa ggagtcctta tgggcgcttt
    13801 tacaggtaag acagttctca tcctcggtgg tagccgtgga atcggtgccg ctatcgtacg
    13861 tcgtttcgtc accgatggag ccaatgtacg attcacctat gcggggtcga aagatgccgc
    13921 tgaacgcctg gcacatgaga ctggatcgac agcagtattc acagatagtg ctgacagaga
    13981 cgctgttatt gatgtcgttc gtaagagcgg cgcattggat atcctggtgg taaatgcagg
    14041 tattggcgtc tttggcgatg ccctggaatt aaatgccgac gatattgatc gccttttcaa
    14101 aatcaatatt catgctcctt atcatgcctc tgttgaagcc gcccggcaga tgcccgaagg
    14161 cgggcgtatc ttaatcatcg gctccgtgaa tggcgatcgt atgcctgttg caggcatggc
    14221 tgcttatgcc gccagtaaat ctgccctgca aggcatggcg cgcgggctgg cccgtgattt
    14281 tggaccgcgt gcgatcacca ttaacgtcgt ccagccagga ccaattgata ccgacgctaa
    14341 tcccgccaac gggccaatgc gcgatatgtt gcatagtttt atggccatca aaagacatgg
    14401 gcaaccggaa gaggtcgctg gtatggtcgc atggttagca gggccagaag ccagttttgt
    14461 tactggcgcg atgcatacca ttgatggcgc atttggcgca taaccgacta cgctcaatta
    14521 agcccagcca ttatccatga tgtctgggtt ttgtttactc acgtcgtccc cctaaaagcg
    14581 gctcccggta aatataaatc ttctgttcat gtcgtaaatc ttgaggataa ccaaatggta
    14641 gaaagaaccg ctgttttccc tgctggccga cattcactgt atgctgagca tcgttattct
    14701 gcggctattc gttccggcga tttgctgttt gtttccgggc aagtaggaag tcgagaggac
    14761 ggaacaccag aacccgattt tcagcaacaa gtcagactgg catttgataa tttgcatgcg
    14821 accctggctg ctgcgggatg cacttttgac gatatcattg atgttacgag ctttcatacc
    14881 gatcctgaaa aacaatttga agacatcatg acagtcaaaa atgaaatatt tagcgcccca
    14941 ccttatccaa actggacggc ggtgggtgtt acctggctgg caggctttga ttttgaaatt
    15001 aaagtgatag cgcgcatccc tgagctgtaa gcaacagtgt tagccgtgag ttttcacact
    15061 ccgccctata agtcggatga atggaataaa atgcatatcg gattgggtga aagtgaaaaa
    15121 gaaaaaagca ggaaatgtct gcaattattg ataccgaacg accgttcccc tccagaacca
    15181 tagaaaataa aaatgcacat actttgactt ttaattcaaa taaaccgttt gcgctgacaa
    15241 aatattgcat caaatgcttg caccgcttct ggctatgagt ataatgccgg acaatttgcc
    15301 gggaggatgt atggttcaat gtgttcgaca ttttgtctta ccgcgtctga aaaaagacgc
    15361 tggcctgccg tttttcttcc cgttgatcac ccattcccag cccctcaatc gaggggcttt
    15421 tttttgccca ggcgtcagga gataaaagat ggctaatccg ctatatcaga aacatatcat
    15481 ttccataaac gaccttagtc gcgatgacct taatctggtg ctggcgacag cggcgaaact
    15541 gaaagcaaac ccgcaaccag agctgttgaa gcacaaagtc attgccagct gcttcttcga
    15601 agcctctacc cgtacccgcc tctctttcga aacttccatg caccgcctgg gtgccagcgt
    15661 ggtgggcttc tccgacagcg ccaatacatc actgggtaaa aaaggcgaaa cgctggccga
    15721 taccatttcg gttatcagca cttacgtcga tgcgatagtg atgcgtcatc cgcaggaagg
    15781 tgcggcgcgc ctggccaccg aattttccgg caatgtaccg gtactgaatg ccggtgatgg
    15841 ctccaaccaa catccgacgc aaaccttgct ggacttattc accattcagg aaacccaggg
    15901 gcgtctggac aatctccacg tcgcaatggt tggtgacctg aaatatggcc gcaccgttca
    15961 ctccctgacc caggcgctag cgaagttcga cggcaaccgt ttttacttta tcgcgccgga
    16021 tgcgctggca atgccgcaat acattctgga tatgctcgat gaaaaaggga tcgcctggag
    16081 tctgcacagc tctattgaag aagtgatggc ggaagtagac atcctgtaca tgacccgcgt
    16141 gcaaaaagag cgtctggacc cgtccgagta cgccaacgtg aaagcgcagt ttgttcttcg
    16201 cgccagcgat ctccacaacg ccaaagccaa tatgaaagtg ctgcatccgc tgccgcgtgt
    16261 tgatgagatt gccaccgatg ttgataaaac gccgcacgcc tggtacttcc agcaggcagg
    16321 caacgggatt ttcgctcgcc aggcgttact ggcactggtt ctgaatcgcg atctggtact
    16381 gtaaggggaa atagagatga cacacgataa taaattgcag gttgaagcta ttaaacgcgg
    16441 cacggtaatt gaccatatcc ccgcccagat cggttttaag ctgttgagtc tgttcaagct
    16501 gaccgaaacg gatcagcgca tcaccattgg tctgaatttg ccttctggcg agatgggccg
    16561 caaagatctg atcaaaatcg aaaatacctt tttaagtgag gatcaggttg atcagctggc
    16621 gttatatgca ccgcaagcca cggttaaccg tatcgacaac tatgaagtgg ttggcaaatc
    16681 gcgtcccagt ctgccagagc gcatcgacaa tgtgctggtc tgcccgaaca gcaactgtat
    16741 cagccatgcc gaaccggttt catccagctt tgccgtgcga aaacgcgcca atgatatcgc
    16801 gctcaaatgc aaatactgtg aaaaagagtt ttctcataat gtggtgctgg ccaattaatt
    16861 gcggttggta ataaaagtct ggctccctat aatgagccag actttttacc gctgtaataa
    16921 aggagaaatc atgagcaaaa ctatcgcgac ggaaaatgca ccggcagcta tcggtcctta
    16981 cgtgcagggc gttgatctgg gcaatatgat tatcacttcc ggtcagatcc cggtaaaccc
    17041 gaaaacgggt gaagtaccgg cagacgtcgc tgcacaggca cgtcagtcgc tggataacgt
    17101 aaaagcgatc gtcgaagccg ctggcctgaa agtgggcgat atcgttaaaa ccaccgtgtt
    17161 tgtgaaagat ctgaacgact tcgcaaccgt taatgctacc tacgaagcct tcttcaccga
    17221 acacaacgcc accttcccgg cacgttcttg cgttgaagta gcccgtctgc cgaaagacgt
    17281 gaagattgag atcgaagcga tcgctgttcg tcgctaatct tgatggaaat ccgggctatc
    17341 atgcccggat taagtctgat gacaaacgca aaatcgcctg atgcgctacg cttatcaggc
    17401 ctacgtgatt cctgcaattt attgaattta ttggccggat aaggcattta cgccgcatcc
    17461 ggcatgaaca aaactcactt tgtctgcaat ctgaatccgg gccaccatgc ccagtttatt
    17521 ctttactgcc aaccgtaacg acggctatag aaccctttca ccaactgggt taacgtcata
    17581 taccctgcca gaatcgcaac cagccacggg aaatagctta acggcagcgc ctgcagttgc
    17641 agataactgg ccagcggtga aaaaggcaat gcgatcccga caatcatcac gatcacggtc
    17701 atgatcatta acggccacga tgcacagctc tgaataaacg gtacacgacg ggtgcggatc
    17761 atatgcacaa tcagcgtttg cgacagtaag cccaccacaa accagcccga ctggaacagc
    17821 gtttgcgttt ccggcgtgtt ggcatggaat acccaccaca tcaggcaaaa cgtcagaata
    17881 tcgaagatcg agctgatcgg tccgaagaag atcataaagc gccccagatc cgccggattc
    17941 caacgctgcg gcttttgaat ttgctcgtcg tcgacgttat caaacgggat cgccacctgt
    18001 gacacatcgt acagcaggtt ctgaataagc aagtgtaacg gcaacatcgg caggaagggc
    18061 aagaaagcac tcgccaccag cacgctgaac acattaccga agttagagct cgccgtcatt
    18121 ttgatgtatt tcagcatgtt ggcgaaagtg cgacgtccct caataacccc ctcttccagc
    18181 accatcaggc ttttttccag caggatgata tcagccgctt cacgggcaat atctaccgcg
    18241 ccgtccacag aaatgccgat atccgcagca cgtaaggcgg gcgcatcatt tataccatcg
    18301 cccataaagc caaccacatg cccttcgcgc ttcagtaagg tcacaatgcg ttctttatgc
    18361 atcggcgtca ggcgagcaaa cagcgtggtg cgctgtgcga gatttgccag ttcgtcgtca
    18421 gatagcgttt caatatcact accaatgacc acctctcccg cgtccagccc cacttcatgg
    18481 cacactttcg ctgcaactaa ctcactgtcg ccagtaagga tttttacggt aatgccactc
    18541 gcttttaatg ccttcagtgc cggagcagtt gtctcttttg gcggatcaag gaaagcaata
    18601 tatccttcga ggatcaggtc ggattcatcc gcccgctggt aatcaccttc acgcgctggc
    18661 aggtatttcg tcgccaccgc aaccacgcgc agcccctgac gattcagcgt atcagtaacc
    18721 cgcttaatct tacgcagcat gatgtcatcg agcggcacaa tctcgccatt gtgacgcacc
    18781 tgcgaacaca cattgaggat ttcctgcaat gcacctttgc aaaccagctg atggtgctcg
    18841 gtattttctg ccactaccac cgacatccgg cgacgctcga aatcaaacgg aatctcatcg
    18901 attttttgcc aacgactggc cagcgagcgc gctgactctt catccgtacc ttcgagcacc
    18961 gctgtatcga gcaggttttt aagcccggtc tggtaatgac tgttcaacca cgcactatgc
    19021 agcacgcgtt cgctagtttt accggagata tcggtatgat tctccagcac aattttatcc
    19081 tgtgtcaggg tgccggtttt atcagtgcac agaatatcca ttgcgccaaa gttctgaatg
    19141 gcatccagat gtttgacgat cactttctgt ttcgacaact tgactgcacc gcgcgccagc
    19201 gtcgaggtaa caatcatcgg caacatttcc ggcgttaagc ctaccgcgac cgaaagcgca
    19261 aacagtgccg cttcccacca gtcgcctttg gtgtaaccat tgattaacag caccaccgga
    19321 gccatcacca gcataaagcg aatcagcagc atactgacgc ggctgatccc ttgctgaaag
    19381 gcattcggct cgctttcctg ctcactaaca cgccccgcca gttgaccaaa ccaggtattg
    19441 gcacctgtgg caatcaccat tgcttgtgcc gtgccgctca ccacggtggt gcccataaaa
    19501 cacagggtgt cgcactccag cggattgctg tgctccggct ggcgagtagt agcggctttt
    19561 tccacgggca gagactcacc ggttaacgac gcctgagcga cgaacagatc ccgcgcctgc
    19621 aagatacgta aatctgccgg gatcatatcc cctgccgcca gttttataat atcgccgggc
    19681 accagctggt cgatcgggat ctccagccag ccattttcgc ctttgtcgtt aattacgcgc
    19741 agcaccgtcg cagtattgct gaccatcgct ttcagggcat ctgccgcttt agtggaacgt
    19801 gcttcctgaa taaagttcag caacgtagaa atagcgacca ttagcgcgat aacggccgcg
    19861 gcaaataaat cttccgtggc gtaagaaata gcgccgagaa tggtgagtaa aatattaaag
    19921 gggttgcgat agcagaccca taaatgaacc caccacggcg acggttgttg tgcgggtaat
    19981 ttattttcac catgttgttc gcgggcagat tccacttccg cctgatttaa tccttccgga
    20041 tgggtgtcga acgttttcca caattcttct tcgggcatca ccgccatttt caggcaatgc
    20101 gcacttaagg acggcgggac cgccgtattc actgtctgct gcgcgccagg caatggatcg
    20161 cgatgaacca gacgggaagg taaatggcga atgaaccggg taaaaatttc tttaaacata
    20221 aggagtccct ccgcactgtc tgaaacagta cagaaatgtc ttcaggcgtg gcgaagcctt
    20281 acctgaaggt aataaagctg agcagggacg ttaaaacgtc acggccttac gcatccggta
    20341 acgccgtgaa aagacgggct taccggaaaa gttttaatct ccgtcgaggg agaggcgtgg
    20401 gatcaggttc catataacct ccggtaagtg aaaaaagcgc cgcaaataat aaggagaatc
    20461 caataatatt cgtagcatct atggcgatat aatacctgct ggcaactaaa ccaacgataa
    20521 accagacttt acccattgct gaatgcacgg gtaaagttag gctcaaataa ttaaacaaca
    20581 cgttacggga caacaggatg caaaatcggc tgaccatcaa agacatcgcg cgcttaagcg
    20641 gcgtggggaa atctacggtt tcccgggtgc tgaataacga aagcggcgtg agccagcgca
    20701 cgcgcgagcg tgttgaagca gtgatgaatc agcatggatt ttccccttcc cgctctgcgc
    20761 gcgctatgcg tgggcaaagc gataaagtgg tcgccatcat tgttacccgt ctggattcgt
    20821 tgtcagaaaa tctcgccgtt caaaccatgc tgccagcgtt ctatgaacaa ggttacgacc
    20881 caatcatgat ggaaagtcag ttttccccgc aattagttgc cgaacatttg gggggcctga
    20941 aacggcgtaa tatcgacggc gtagtgctgt tcggttttac cggcataaca gaagaaatgt
    21001 tagcccactg gcagtcatcg ctggttctgc tggcgcgtga cgcaaaaggc tttgcttcag
    21061 tctgttatga cgacgaaggg gcaatcaaga tcctgatgca acggctgtat gaccaggggc
    21121 atcgtaatat cagttatctc ggcgtgccgc atagtgacgt gacgaccggt aagcgacgtc
    21181 acgaagccta cctggcgttc tgcaaagcgc ataaattgca tcccgttgcc gctctgccag
    21241 ggcttgctat gaagcaaggc tatgagaacg tagcaaaagt gattacgcct gaaactaccg
    21301 cgttactgtg cgcaaccgac acgctggcac ttggcgcaag taaatacctg caagagcaac
    21361 gcatcgacac cttgcaactg gcgagcgtcg gtaatacacc gttaatgaaa ttcctccatc
    21421 cggagatcgt aaccgtagat cccggttacg ccgaagctgg acgccaggcg gcctgccagt
    21481 tgatcgcgca ggtcaccggg cgcagcgaac cgcaacaaat catcatcccc gccaccctgt
    21541 cctgatcgtt tcctgaacga taaattgtga tcttcgctgc gtttcgggaa cgttcccgtt
    21601 tttaaatttt tccgcgcaat atattctgca gccaaccaaa aatgtcatct gccatggggc
    21661 tttatgatga gcaaaataaa ccaaacggat atcgatcggt tgattgaact ggtcggcggg
    21721 cgcggcaata ttgcgacggt gagccactgt attactcgtc tgcgctttgt cctcaaccaa
    21781 ccggccaatg ccagaccgaa agaaattgag caactcccca tggtgaaagg ctgtttcacc
    21841 aatgccgggc aatttcaggt ggtgattggc accaacgtgg gtgattacta tcaagcactg
    21901 attgcgtcaa ccggacaggc gcaggttgat aaagagcagg taaaaaaagc cgcccggcag
    21961 aatatgaaat ggcatgagca gttgatctct catttcgcgg agatcttctt cccgttgctg
    22021 cccgcgttga ttagtggtgg tttgatcctc ggttttcgca atgtgatcgg cgatttgccc
    22081 atgagcaacg gtcaaacgct ggcgcaaatg tacccttccc tgcaaacgat ctacgatttt
    22141 ctatggttga tcggtgaagc gatcttcttc tacttgccgg tcgggatttg ctggtcagcg
    22201 gtgaaaaaaa tgggcggtac accgatcctc ggtatcgtgc ttggcgtgac actggtttct
    22261 ccacagctga tgaacgctta tctgctcggg cagcagctgc cggaagtgtg ggactttggc
    22321 atgttcagca tcgctaaagt gggctatcag gcgcaggtga tcccggcact gttagccggg
    22381 ttggcgctgg gcgttattga aactcgcctt aaacgcatcg taccggatta cctctatctg
    22441 gtggtggtgc ccgtctgttc gctgatcctc gcggtgttcc tcgcccatgc gctgattggt
    22501 ccgtttggtc gcatgattgg cgatggcgtt gcctttgcgg tacgtcacct gatgaccggc
    22561 agctttgctc cgattggcgc ggcattgttt ggcttcctgt acgccccgct ggtgatcacc
    22621 ggcgtacacc agaccacgct tgctattgat ttgcagatga ttcaaagcat gggtggcacg
    22681 ccagtgtggc cgctgattgc gctgtcgaat atcgctcagg gctccgctgt gataggtatt
    22741 atcatttcca gccgcaagca caatgaacgc gagatctccg tgcctgcggc gatctccgcc
    22801 tggcttgggg tcactgagcc tgcaatgtac ggcatcaacc tgaaatatcg cttcccgatg
    22861 ctgtgcgcga tgattggttc tggtctggca ggattgctat gcggcctgaa cggcgttatg
    22921 gcgaatggca tcggcgtagg cggcctgccg ggaattctct cgattcaacc gagctactgg
    22981 caggtgtttg cgctggcaat ggctatcgcc atcatcatcc cgattgtact cacctcgttt
    23041 atctatcagc ggaaataccg cctgggcacg ctggacattg tttaattttc ttcggggcgc
    23101 aattgcgccc cctcgcattt gcaggaataa cgtaatgact catcttcccc actggtggca
    23161 aaacggcgtt atctaccaga tttatccaaa gagttttcag gacaccacgg gtagcggcac
    23221 cggcgattta cgtggcgtta tccagcgcct ggactatctg cataaactgg gcgttgatgc
    23281 catctggcta accccctttt atgtctctcc ccaggtcgat aacggttacg acgtagcaaa
    23341 ttatacggcg attgatccca cctatggcac gctggacgat tttgacgaac tggtgacaca
    23401 ggcaaaatcg cgtgggattc gtatcattct cgatatggtg tttaaccata cctctaccca
    23461 acatgcctgg tttcgcgagg cgctgaacaa agaaagccct taccgccagt tttatatctg
    23521 gcgcgatgga gaaccagaaa cgccaccgaa caactggcgt tcaaaattcg gtggtagtgc
    23581 gtggcgctgg catgcggaaa gcgaacagta ctatttgcat ctctttgcac cagaacaggc
    23641 ggatctcaac tgggagaatc cagcggtacg cgcagagctg aaaaaagtct gtgagttctg
    23701 ggccaatcgt ggggtcgacg ggttgcgcct ggatgtggtg aatctgatct ccaaagatcc
    23761 gcgttaccct gaagacctgg atggcgacgg gcgtcgcttc tacaccgacg ggccacgagc
    23821 acacgagttt ttgcacgaga tgaaccgcga tgtgtttacg ccacacgggt taatgaccgt
    23881 aggtgaaatg tcctccacca gccttgagca ttgccagcga tacgcggcac tgacaggcag
    23941 tgaattgtcg atgaccttta attttcatca cctgaaggtc gattatcccg gtggtgagaa
    24001 atggacgctg gctaaacctg actttgtggc gttgaaaaca ttgttccgcc actggcaaca
    24061 aggaatgcac aacgtggcat ggaatgcctt gttctggtgt aaccacgatc agccgcgcat
    24121 tgtttctcgc tttggtgatg aaggtgaata ccgcgtgcct gcggcaaaaa tgctggcgat
    24181 ggtgctgcat ggcatgcagg gaacgccgta tatctaccag ggcgaagaga ttggcatgac
    24241 caacccacat ttcacgcgca ttactgacta tcgcgacgtg gagagcctca atatgtttgc
    24301 cgagctgcgc aacgatggtc gtgatgccga cgagttattg gcaatcctcg ccagtaaatc
    24361 ccgtgacaac agtcgcacac ccatgcaatg gaccaacggc gataatgccg ggtttacggc
    24421 tggcgaaccg tggattggcc tgggcgataa ctatcaggaa atcaacgtag aagcagcgct
    24481 ggccgatgag tcctcggtgt tttacaccta ccaaaagtta atcgcactgc gtaagcaaga
    24541 agccgtcctg acatggggcg attaccagga tctgctgcca aacagccctg tattgtggtg
    24601 ctatcgccgt gagtggaagg ggcaaacctt gctggtcatt gccaacctta gccgtgagac
    24661 ccaaccctgg cagccaggga aaatgctcgg caactggcag cttgtgatgc ataactacga
    24721 agaagcctca ccacaaccct gtgccatgac tttacggcct tttgaggctg tctggtggtt
    24781 acagaagtaa atcttcgcta agccccggta atgcctgtca ttccggggtt ttgcctgtca
    24841 gcatgttgtt tttgttgatt ttataatcaa aaaattcatt aaaaaattta catatcgctg
    24901 tagcgcccgt catccgtacg ctctgctttt tactttgagc tacatcaaaa aaagttcaaa
    24961 catccttgat gcaaagcact atatatagac tttaaaatgc gtcccaaccc aatatgttgt
    25021 attaatcgac tataattgct actacagctc cccacgaaaa aggtgcggcg ttgtggataa
    25081 gcggatggcg attgcggaaa gcaccggaaa acgaaacgaa aaaaccggaa aacgcctttc
    25141 ccaatttctg tggataacct gttcttaaaa atatggagcg atcatgacac cgcatgtgat
    25201 gaaacgagac ggctgcaaag tgccgtttaa atcagagcgc atcaaagaag cgattctgcg
    25261 tgcagctaaa gcagcggaag tcgatgatgc cgattattgc gccactgttg ctgcggttgt
    25321 cagcgagcag atgcagggcc gcaaccaggt ggatatcaat gagatccaga ccgcagttga
    25381 aaaccagctg atgtcggggc catacaaaca actggctcgt gcttacatcg agtaccgtca
    25441 cgatcgcgac attgaacgtg aaaaacgcgg tcgcctgaac caggagatcc gtggtctggt
    25501 cgagcagacc aactcctcgt tactcaacga aaacgccaac aaagacagca aggtgattcc
    25561 aacccagcgc gacctgctgg ccgggatcgt ggctaaacac tatgcacgcc agcacttgct
    25621 gccgcgtgac gtggtgcagg cacatgagcg tggcgatatt cactatcacg atctcgatta
    25681 ctcgccgttc ttcccgatgt tcaactgcat gttgatcgac ctgaaaggca tgctgaccca
    25741 gggctttaag atggggaacg ccgagattga accgccgaag tcgatctcta cggcaaccgc
    25801 ggtaactgcg cagattattg ctcaggtcgc cagccatatt tatggcggca ccaccattaa
    25861 ccgtatcgat gaagtgctgg caccgtttgt caccgccagc tacaacaaac atcgcaagac
    25921 cgcggaagag tggaacatcc cggacgccga aggctacgct aactctcgta ccatcaaaga
    25981 gtgctacgat gccttccagt cgctggagta cgaagtaaac accctgcata ccgccaacgg
    26041 tcaaacgccg tttgtaacct ttggttttgg tctgggcacc agctgggaat cgcgcctgat
    26101 tcaggaatcg atcctgcgta accgtatcgc aggcctcggt aaaaaccgta aaacggcggt
    26161 gttcccgaaa ctggtgtttg cgattcgcga tggcctgaac cataaaaaag gcgatccgaa
    26221 ctacgacatc aaacagctgg cgctggagtg cgcaagcaag cgcatgtatc cggatatcct
    26281 gaactacgat caggtagtga aagtcaccgg ttcgtttaaa accccgatgg gctgccgcag
    26341 cttcctcggc gtgtgggaaa atgaaaacgg cgagcagatc cacgatggtc gtaacaacct
    26401 cggcgtgatc agcctgaacc tgccgcgtat tgctctggag gcaaaaggcg atgaagccac
    26461 cttctggaag ctgctggatg aacgtctggt gctggcacgt aaggcgctga tgacccgtat
    26521 cgctcgtctc gaaggcgtga aagcgcgcgt agccccgatc ctctatatgg aaggtgcttg
    26581 tggcgtgcgt ctgaatgctg acgatgatgt ttctgaaatc ttcaaaaacg gtcgtgcgtc
    26641 tatttcgctg ggttacatcg gcatccacga aaccattaac gcgctgtttg gcggcgagca
    26701 tgtgtatgac aacgagcagc tgcgcgcgaa aggtatcgcc attgttgaac gtctgcgtca
    26761 ggcggtggat cagtggaaag aagaaacggg ttacggtttt agcctttaca gcacgccaag
    26821 tgaaaacctg tgcgaccgct tctgccgtct cgataccgct gagtttggcg tggtgccggg
    26881 cgtgactgat aaaggttact acaccaacag tttccacctc gatgtggaga agaaggtgaa
    26941 cccgtacgac aagatcgact ttgaagcgcc ttacccgccg ctggcgaacg gtggtttcat
    27001 ttgctacggc gaatatccaa acattcagca caacctgaag gcgctggaag atgtctggga
    27061 ttacagctat cagcatgtac cgtattacgg caccaatacg ccgattgatg agtgttacga
    27121 gtgtggtttt accggtgagt ttgagtgcac cagtaaaggc ttcacctgcc cgaaatgtgg
    27181 taaccatgac gcctcccgtg tgtcggtgac tcgccgcgtg tgcggatatt taggtagccc
    27241 ggatgcgcgt ccgtttaacg ctggtaagca ggaagaagtt aagcgccgcg ttaaacattt
    27301 ggggaatggg cagataggtt aatccttccc cctcacccta accctctccc tcaagggaga
    27361 ggggaccgtt ccgtgcccct tcctgattcc cgacataatg aattgcctct ggccagtttt
    27421 tgtcgtcgca aggaggcaaa tgcgtagcat cgggggactt cgcccgtacg caccagagca
    27481 ttcatggcgt caattgagaa gcgaaaatga actttcatca gtactatcct gtcgacatcg
    27541 tcaacggtcc cggcactcgc tgcaccctgt ttgtctccgg gtgtgttcat gaatgccccg
    27601 gttgctataa caaaagcaca tggcgggtaa attccggtca gccatttacc aaagcaatgg
    27661 aagaccagat cattaacgat ctgaatgaca ctcgtatcaa acgccagggg atttccctct
    27721 ccggcggcga tccgctgcat ccgcaaaacg tgccggatat tctgaaactg gtacaacgca
    27781 tccgcgccga gtgtccgggt aaagacatct gggtgtggac aggctataaa ctcgacgaac
    27841 tcaacgctgc gcaaatgcag gttgttgatt tgattaacgt gctggtcgac ggcaaattta
    27901 tgcaggatct aaaagaccct tccctgatct ggcgcggcag cagcaatcag gtggtgcatc
    27961 atttacgtta gtcactcatc cagccgtttt gtcgcgtctt cataaacggc tgagttttct
    28021 ctcttaccaa tagctatcac caacacgagt aactctttat cattgacgga atagaccagg
    28081 cgatatccca gtgtgcgcag cttaattttg tagcagtccg gtaaatcccg cagtttagcc
    28141 gatggattgc gcggcctttt cagcacatcc tcaagctttt ttttaaattg ttcccggact
    28201 gtcacgccca gtttttgcca ctctttcagc gcccgcggat caaacgccag ctcatagttc
    28261 atccagtcgg accttaatgg cctcttgtgg gttggccagt cgttccctga cagtgcgtaa
    28321 caaggcttct tcactatcac tgacagtgag cgtttttacc ggcattcgtc cattttccgc
    28381 aacgtactga aacagcagcc gcactgcttc agtaggcgta atatttaatt tttccagcac
    28441 cgcataagcc tcatttttca gtttgtcatc caggcggacg ttaagtgtgg ccattatgcc
    28501 tccggtaatt atgaaaagtg taatgcatta tagcattaca tatgcattac attcatttct
    28561 ggaaaacgcc tcgcaaaaat gaggacgaca cgcaggaata tgccgtggtg aagatagata
    28621 ttgcggtgat tttacgaagg gagaagaaag cgttgggcgg taacgtttca ccgcccaata
    28681 aggaattaac gatacttctg gtgataggcg ttgcgggtcg ttttcagttg ctctgcggca
    28741 gcctgcgctt cttttacttt accttcattt gccagcttca gcgcgtcgtc aatctgaccg
    28801 accagaatgt cgaaaccgtg gcggaagtct ttcatttccg ggctgtccgg tgatttgcct
    28861 tcaagcttcg gcggcgtcgc tttttgcgca tccagcgccg cagcgcgcat tttagttaac
    28921 gcgtctttga cttgcaccgc gttatcggct ttttcgacca cttttaaatt gtcgttgagg
    28981 gtttccatat tgtcttccag
//
LOCUS       DAMZFT010000055        28750 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.055, whole genome shotgun sequence.
ACCESSION   DAMZFT010000055 DAMZFT010000000
VERSION     DAMZFT010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 28750)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28750)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28750
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.055"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..886
                     /gene="gshB"
                     /locus_tag="PN977_002494"
     CDS             <1..886
                     /gene="gshB"
                     /locus_tag="PN977_002494"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HDI6042516.1"
                     /translation="AMLLEAQRRGYELHYMEMGDLYLINGEARAHTRTLNVKQNYEEW
                     FSFVGEQDLLLADLDVILMRKDPPFDTEFIYATYILERAEEKGTLIVNKPQSLRDCNE
                     KLFTAWFSDLTPETLVTRNKAQLKAFWEKHSDIILKPLDGMGGASIFRVKEGDPNLGV
                     IAETLTEHGTRYCMAQNYLPAIKDGDKRVLVVDGEPVPYCLARIPQGGETRGNLAAGG
                     RGEPRPLTESDWKIARQIGPTLKEKGLIFVGLDIIGDRLTEINVTSPTCIREIEAEFP
                     VSITGMLMDAIEARLQQQ"
     gene            995..1558
                     /locus_tag="PN977_002495"
     CDS             995..1558
                     /locus_tag="PN977_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005228750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HDI6042517.1"
                     /translation="MNLQHHFLIAMPALQDPIFRRSVVYICEHNTNGAMGIIVNKPLE
                     NLKIEGILEKLKITPEPRDESIRLDKPVMLGGPLAEDRGFILHTPPSNFASSIRISDN
                     TVMTTSRDVLETLGTDKQPSDVLVALGYASWEKGQLEQEILDNAWLTAPADLNILFKT
                     PIADRWREAAKLIGVDILTMPGVAGHA"
     gene            1558..1974
                     /gene="ruvX"
                     /locus_tag="PN977_002496"
     CDS             1558..1974
                     /gene="ruvX"
                     /locus_tag="PN977_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HDI6042518.1"
                     /translation="MSGTLLAFDFGTKSIGVAVGQRITGTARPLPAIKAQDGTPDWNI
                     IERLLKEWQPDEIIVGLPLNMDGTEQPLTARARKFANRIHGRFGVEVKLHDERLSTVE
                     ARSGLFEQGGYRALNKGKVDSASAVIILESYFEQGY"
     gene            complement(2158..3138)
                     /locus_tag="PN977_002497"
     CDS             complement(2158..3138)
                     /locus_tag="PN977_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HDI6042519.1"
                     /translation="MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGRMEAAPFDTPDV
                     EELLREWLDDDQRAILLENGQLDFAVSLAENQRLRGSAFAQRHGISLALRLLPSHCPQ
                     LEQLGAPTVLPELLKSENGLILVTGATGSGKSTTLAAMVGYLNQHADAHILTLEDPVE
                     YLYASQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETG
                     HLVLATLHTRGAAQAVERLVDSFPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVAL
                     FELLINTPAVGNLIREGKTHQLPHVIQTGQQVGMITFQQSYQHRVGEGRL"
     gene            3156..3914
                     /gene="yggS"
                     /locus_tag="PN977_002498"
     CDS             3156..3914
                     /gene="yggS"
                     /locus_tag="PN977_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate homeostasis protein"
                     /protein_id="HDI6042520.1"
                     /translation="MNDIAHNLAQVRDKISAAATRCGRSPEEITLLAVSKTKPASAIA
                     EGIDAGQRQFGENYVQEGVDKIRHFQELGVTGLEWHFIGPLQSNKSRLVAEHFDWCHT
                     IDRLRIATRLNDQRPAELPPLNVLIQINISDENSKSGIQLAELDELAAAVAELPRLRL
                     RGLMAIPAPESEYVRQFEVARQMAVAFAGLKTRYPHIDTLSLGMSDDMEAAIAAGSTM
                     VRIGTAIFGARDYSKNKELKERHEYVDFPAFNGH"
     gene            3877..4443
                     /gene="yggT"
                     /locus_tag="PN977_002499"
     CDS             3877..4443
                     /gene="yggT"
                     /locus_tag="PN977_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotic shock tolerance protein YggT"
                     /protein_id="HDI6042521.1"
                     /translation="MNTLTFLLSTVIELYTMVLLLRIWMQWAHCDFYNPFSQFVVKVT
                     QPIIGPLRRVIPAMGPIDSASLLVAYILSFIKAIVLFKVVTFLPIIWIAGLLILLKTI
                     GLLIFWVLLVMAIMSWVSQGRSPIEYVLIQLADPLLRPIRRLLPAMGGIDFSPMILVL
                     LLYVINMGVAEVLQATGNMLLPGLWMAL"
     gene            4440..4730
                     /locus_tag="PN977_002500"
     CDS             4440..4730
                     /locus_tag="PN977_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggU family protein"
                     /protein_id="HDI6042522.1"
                     /translation="MNAVTVNDDGLVLRLYIQPKASRDSIVGLHGDEVKVAITAPPVD
                     GQANSHLVKFLGKQFRVAKSQVVIEKGELGRHKQIKIINPQQIPPEIAALIN"
     gene            4738..5331
                     /gene="rdgB"
                     /locus_tag="PN977_002501"
     CDS             4738..5331
                     /gene="rdgB"
                     /locus_tag="PN977_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HDI6042523.1"
                     /translation="MQKVVLATGNVGKVRELASLLSDFGLDIVAQTDLGVDSAEETGL
                     TFIENAILKARHAAKVTALPAIADDSGLAVDVLGGAPGIYSARYSGEDATDQKNLQKL
                     LETMKDVPDDQRQARFHCVLVYLRHAEDPTPLVCHGSWPGVITREPAGTGGFGYDPIF
                     FVPSEGKTAAELTREEKSAISHRGQALKLLLDALRNG"
     gene            5324..6460
                     /gene="hemW"
                     /locus_tag="PN977_002502"
     CDS             5324..6460
                     /gene="hemW"
                     /locus_tag="PN977_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HDI6042524.1"
                     /translation="MVKLPPLSLYIHIPWCVQKCPYCDFNSHALKGEVPHDDYVQHLL
                     NDLDNDVAYAQGREVKTIFIGGGTPSLLSGPAMQTLLNGVRARLPLAADAEITMEANP
                     GTVEADRFVDYQRAGVNRISIGVQSFSEEKLKRLGRIHGPQEAKRAAKLASGLGLRSF
                     NLDLMHGLPDQSLEEALGDLRQAIELNPPHLSWYQLTIEPNTLFGSRPPVLPDDDALW
                     DIFEQGHQLLTAAGYQQYETSAYAKPGYQCQHNLNYWRFGDYIGIGCGAHGKVTFPDG
                     RILRTTKTRHPRGFMQGRYLESQRDVEATDKPFEFFMNRFRLLEAAPRVEFIAYTGLC
                     EDVIRPQLDEAIAQGYLTECADYWQITEHGKLFLNSLLELFLAE"
     gene            complement(6615..7622)
                     /locus_tag="PN977_002503"
     CDS             complement(6615..7622)
                     /locus_tag="PN977_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1202 family protein"
                     /protein_id="HDI6042525.1"
                     /translation="MKKQWIVGTALLMLMTGNAWADGEPPTENILKDQFKKQYHGILK
                     LDAITLKNLDAKGNQATWSAEGDVSSSDDLYTWVGQLADYELLEQTWTKDKPVKFSAM
                     LTSKGTPASGWSVNFYSFQAAASDRGRVVDDIKTNNKYLIVNSEDFNYRFSQLESALN
                     TQKNSIPALEKEVKALDKQMVAAQKAADAYWGKDANGKQMTREDAFKKIHQQRDEFNK
                     QNDSEAFAVKYDKEVYQPAIAACHKQSEECYEVPIQQKRDFDINEQRRQTFLQSQKLS
                     RKLQDDWVTLEKGQYPLTMKVSEINSKKVAILMKIDDINQANERWKKDTEQLRRNGVI
                     K"
     gene            complement(7739..8785)
                     /gene="ansB"
                     /locus_tag="PN977_002504"
     CDS             complement(7739..8785)
                     /gene="ansB"
                     /locus_tag="PN977_002504"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparaginase 2"
                     /protein_id="HDI6042526.1"
                     /translation="MEFFKKTALAALVMGFSGAALALPNITILATGGTIAGGGDSATK
                     SNYTAGKVGVENLVNAVPQLKDIANVKGEQVVNIGSQDMNDNVWLTLAKKINTDCDKT
                     DGFVITHGTDTMEETAYFLDLTVKCDKPVVMVGAMRPSTSMSADGPFNLYNAVVTAAD
                     KASANRGVLVVMNDTVLDGRDVTKTNTTDVATFKSVNYGPLGYIHNGKIDYQRTPARK
                     HTSDTPFDVSKLNELPKVGIVYNYANASDLPAKALVDAGYDGIVSAGVGNGNLYKSVF
                     DTLATAAKTGTAVVRSSRVPTGATTQDAEVDDAKYGFVASGTLNPQKARVLLQLALTQ
                     TKDPQQIQQIFNQY"
     gene            complement(8961..9680)
                     /locus_tag="PN977_002505"
     CDS             complement(8961..9680)
                     /locus_tag="PN977_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2884 domain-containing protein"
                     /protein_id="HDI6042527.1"
                     /translation="MMRKMLLAAALSVTAMTAHADYQCSVTPRDDVIVSPQTVQVKGE
                     NGNLVITPDGNVMYNGKQYSLNAAQREQAKDYQAELRSTLPWIDEGAKSRVEKARIAL
                     DKIIVQEMGESSKMRSRLTKLDAQLKEQMNRIIETRSDGLTFHYKAIDQVRAEGQQLV
                     NQAMGGILQDSINEMGAKAVLKSGGNPLQNVLGSLGGLQSSIQTEWKKQEKDFQQFGK
                     DVCSRVVTLEDSRKALVGNLK"
     gene            complement(9864..10190)
                     /locus_tag="PN977_002506"
     CDS             complement(9864..10190)
                     /locus_tag="PN977_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggL family protein"
                     /protein_id="HDI6042528.1"
                     /translation="MAKNRSRRLRKKMHIDEFQELGFSVAWRFPEGTSEEQIDKTVDD
                     FINEVIEPNKLAFDGSGYLAWEGLICMQEIGKCTEEHQAIVRKWLEERKLDEVRTSEL
                     FDVWWD"
     gene            complement(10190..10909)
                     /gene="trmB"
                     /locus_tag="PN977_002507"
     CDS             complement(10190..10909)
                     /gene="trmB"
                     /locus_tag="PN977_002507"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HDI6042529.1"
                     /translation="MKNDVISPEFDENGRPLRRIRSFVRRQGRLTKGQEHALENYWPV
                     MGVEFSEDMLDFPALFGREAPVTLEIGFGMGASLVAMAKDRPEQDFLGIEVHSPGVGA
                     CLASAHEEGLSNLRVMCHDAVEVLHKMIPDNSLRMVQLFFPDPWHKARHNKRRIVQVP
                     FAELVKSKLQLGGVFHMATDWEPYAEHMLEVMSSIDGYKNLSESNDYVPRPASRPVTK
                     FEQRGHRLGHGVWDLMFERVK"
     gene            11070..12122
                     /gene="mutY"
                     /locus_tag="PN977_002508"
     CDS             11070..12122
                     /gene="mutY"
                     /locus_tag="PN977_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HDI6042530.1"
                     /translation="MQASQFSAQVLDWYDKYGRKTLPWQIDKTPYKVWLSEVMLQQTQ
                     VATVIPYFERFMARFPTVTDLANAPLDEVLHLWTGLGYYARARNLHKAAQQVATLHGG
                     KFPETFEEVAALPGVGRSTAGAILSLSLGKHFPILDGNVKRVLARCYAVSGWPGKKEV
                     ENKLWSLSEQVTPAVGVERFNQAMMDLGAMICTRSKPKCSLCPLQNGCIAAANNSWAL
                     YPGKKPKQTLPERTGYFLLLQHEDEVLLAQRPPSGLWGGLYCFPQFADEESLRQWLAQ
                     RQIAADNLTQLTAFRHTFSHFHLDIVPMWLPVSSFTGCMDEGNALWYNLAQPPSVGLA
                     APVERLLQQLRTGAPV"
     gene            12150..12425
                     /locus_tag="PN977_002509"
     CDS             12150..12425
                     /locus_tag="PN977_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HDI6042531.1"
                     /translation="MSRTIFCTFLQREAEGQDFQLYPGELGKRIYNEISKEAWAQWQH
                     KQTMLINEKKLNMMNAEHRKLLEQEMVNFLFEGKEVHIEGYTPEDKK"
     gene            12490..13569
                     /gene="mltC"
                     /locus_tag="PN977_002510"
     CDS             12490..13569
                     /gene="mltC"
                     /locus_tag="PN977_002510"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltC"
                     /protein_id="HDI6042532.1"
                     /translation="MKKYLALALIAPLLISCSTTKKGDTYNEAWVKDTNGFDILMGQF
                     AHNIENIWGFKEVVIAGPKDYVKYTDQYQTRSHINFDDGTITIETIAGTEPAAHLRRA
                     IIKTLLMGDDPSSVDLYSDVDDITISKEPFLYGQVVDNTGQPIRWEGRASNFADYLLK
                     NRLKSRSNGLRIIYSVTINMVPNHLDKRAHKYLGMVRQASRKYGVDESLILAIMQTES
                     SFNPYAVSRSDALGLMQVVQHTAGKDVFRSQGKSGTPSRSFLFDPASNIDTGTAYLAM
                     LNNVYLGGIDNPTSRRYAVITAYNGGAGSVLRVFSNDKIQAANIINTMTPGDVYQTLT
                     TRHPSAESRRYLYKVNTAQKSYRRR"
     gene            13771..15027
                     /gene="nupG"
                     /locus_tag="PN977_002511"
     CDS             13771..15027
                     /gene="nupG"
                     /locus_tag="PN977_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupG"
                     /protein_id="HDI6042533.1"
                     /translation="MNLKLQLKILSFLQFCLWGSWLTTLGSYMFVTLKFDGASIGAVY
                     SSLGIAAVFMPALLGIVADKWLSAKWVYAICHTIGAITLFMAAQVTTPEAMFLVILIN
                     SFAYMPTLGLINTISYYRLQNAGMDIVTDFPPIRIWGTIGFIMAMWVVSLSGFELSHM
                     QLYIGAALSAILVLFTLTLPHIPVAKQQANQSWTTLLGLDAFALFKNKRMAIFFIFSM
                     LLGAELQITNMFGNTFLHSFDKDPMFASSFIVQHASIIMSISQISETLFILTIPFFLS
                     RYGIKNVMMISIVAWILRFALFAYGDPTPFGTVLLVLSMIVYGCAFDFFNISGSVFVE
                     KEVSPAIRASAQGMFLMMTNGFGCILGGIVSGKVVEMYTQNGITDWQTVWLIFAGYSV
                     VLAFAFMAMFKYKHVRVPTGTQTVSH"
     gene            complement(15077..17212)
                     /gene="speC"
                     /locus_tag="PN977_002512"
     CDS             complement(15077..17212)
                     /gene="speC"
                     /locus_tag="PN977_002512"
                     /EC_number="4.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417440.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase"
                     /protein_id="HDI6042534.1"
                     /translation="MKSMNIAASSELVSRLSTHRRVVALGDTDFTDVAAVVITAADSR
                     SGILALLKRTGFHLPVFLYSEHAVELPAGVTAVINGNEQQWLELESAACQYEENLLPP
                     FYDTLTQYVEMGNSTFACPGHQHGAFFKKHPAGRHFYDFFGENVFRADMCNADVKLGD
                     LLIHEGSAKDAQKFAAKVFHADKTYFVLNGTSAANKVVTNALLTRGDLVLFDRNNHKS
                     NHHGALIQAGATPVYLEASRNPFGFIGGIDAHCFNEEYLRQQIRDVAPEKADLPRPYR
                     LAIIQLGTYDGTVYNARQVIDTVGHLCDYILFDSAWVGYEQFIPMMADSSPLLLELNE
                     NDPGIFVTQSVHKQQAGFSQTSQIHKKDNHIRGQARFCPHKRLNNAFMLHASTSPFYP
                     LFAALDVNAKIHEGESGRRLWAECVEIGIEARKAILARCKLFRPFIPPVVDGKLWQDY
                     PTSVLASDRRFFSFEPGAKWHGFEGYAADQYFVDPCKLLLTTPGIDAETGEYSDFGVP
                     ATILAHYLRENGIVPEKCDLNSILFLLTPAESHEKLAQLVAMLAQFEQHIEDDSPLVE
                     VLPSVYNKYPVRYRDYTLRQLCQEMHDLYVSFDVKDLQKAMFRQQSFPSVVMNPQDAH
                     SAYIRGDVELVRIRDAEGRIAAEGALPYPPGVLCVVPGEVWGGAVQRYFLALEEGGNL
                     LPGFSPELQGVYSETDADGVKRLYGYVLK"
     gene            17605..18312
                     /locus_tag="PN977_002513"
     CDS             17605..18312
                     /locus_tag="PN977_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HDI6042535.1"
                     /translation="MVIGPFINASAVLLGGVLGALLSQRLPERIRVSMTSIFGLASLG
                     IGILLVVKCANLPAMVLATLLGALIGEMCLLEKGVNTAVTKAQNLFRHSRKKPAHESF
                     IQNYVAIIVLFCASGTGIFGAMNEGMTGDPSILIAKSFLDFFTAMIFACSLGIAVSVI
                     SIPLLIIQLTLAWAAALILPLTTPSMMADFSAVGGLLLLATGLRICGIKMFPVVNMLP
                     ALLLAMPLSAAWTAWFA"
     gene            18418..18493
                     /locus_tag="PN977_002514"
     tRNA            18418..18493
                     /locus_tag="PN977_002514"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:18451..18453,aa:Phe,seq:gaa)
     gene            complement(18690..19679)
                     /locus_tag="PN977_002515"
     CDS             complement(18690..19679)
                     /locus_tag="PN977_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001696146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDI6042536.1"
                     /translation="MKINNADKCWNDLMDEFFFCRSVREATEWSYRKVLNGFRKFVGG
                     TLLPEDIRQQHVREWRREVLKNQKRSTHTWNNKVRHMRAIFSFASSSELLHLSCNPFE
                     GMSVRQEKKRKKTLTRKQIKRILLVLEQFQEDEMNLKMHWKCAIRPAWYWIIVVNTLR
                     FTGMRLNQLLHLRLRDVNPEERWIDLCSEGSKTHHEWRIPMVSHLVPGITRLMDEAVK
                     RGATPDDYLFHYHRYALPVSEALSVSGPPEEQKVKGFFRRLSKECGFQVSAHRFRHTL
                     ASTLMAAPERNLYMVKTMLGHRNVSTTLEYVEMDLKGTARILEAELSKLTDDL"
     gene            complement(19733..21337)
                     /locus_tag="PN977_002516"
     CDS             complement(19733..21337)
                     /locus_tag="PN977_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006858204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraI domain-containing protein"
                     /protein_id="HDI6042537.1"
                     /translation="MLPGTKGTEPGVVAPVIPAGFYAPLTAEALLSPEHRELRLRQVW
                     DNNPMPRDVYDRMCLAPLSRLLLNTQNVPATREGRWSRAGGFGDLTVLYTTYAVRLAR
                     GYMFPPDATPEDQAAQAAVWHTVIFWSALFYHLPLLAHLEGELLSGRGWQPGISVPDE
                     PFRFRFRKTAPQGTEAQQLAALTAGTLLPDGATAWLVTAPGALQNLAGALWHQHPGMA
                     LIRDVLQEAARQTESPLNTCAVTAPVTAEASADIRPADPVVTGSTDSVMAVTEVQPAE
                     EVKTESVQTETEGGGESAGASEVPSAQQELTVQTAPVVTADDEAVSASPADQDTAMLL
                     SLFSADESETAERDNGDVVMLADEEEAQDLTDGPDEGDSSPGSIHESPYLVESDASVN
                     QSVSGEYRATEVNPLTGDDPDLKGETFLDWLKAGLNSGEISFNQKGDKIHIVAGLVFL
                     PVPGIFFDYLKAVQLPGDKRPIVQSSFERLCINKRRDDQRFYFARIAEEPGNCARYKT
                     VKGYLVKGRSLMHESKLKDSPYLFIS"
     gene            complement(21351..22859)
                     /locus_tag="PN977_002517"
     CDS             complement(21351..22859)
                     /locus_tag="PN977_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000056182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HDI6042538.1"
                     /translation="MSLNPTDEQSAIIHWQGEKLVVNAFAGTGKTSTLVQFAAACPES
                     RMLYLAYNRAIRDEAERRFPFNVECKTSHQLAYSRFGKHFRHRLVPGLSVTDVARKLN
                     TRYWTLARVAMTALNQFICSADVAPGMQHMPPPDEMKGMSPQDALRAVQILWHEMSNP
                     DGNFPVTHDTYLKLYQLSSPDLSRRWDTILFDEAQDANPVTSALVLGQRCRVVLVGDR
                     YQQIYRFRGASNALSHPALNGADRLWLTQSFRFGPAVAGVASQLLKRAGESREVTGRG
                     GDDEVLTAGEMRARVKAGKMTGHRAILSRTVAGVIGTALMAAMRGRKVYWVGGIEGYR
                     TEALEDLYWFQMDMPERMHSDRLRKDYRGFDEYRHIAKVTKDVEMNQSIRLLELCFPL
                     PKKLELLRKHTVTNEQEADITVSTAHRSKGLEWSLVVLDDDFQDITDPLISEQERRDE
                     TNLLYVAVTRARRTLVLNELMQQLTEAAGDGGAPGNAPVQGKEDDGVEGEDV"
     gene            23386..23604
                     /locus_tag="PN977_002518"
     CDS             23386..23604
                     /locus_tag="PN977_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000127557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1187 family protein"
                     /protein_id="HDI6042539.1"
                     /translation="MTATTDTTPHKYRIEATILKPGGAPVSWVRFQNTPMTEPECVRL
                     FSKEKEAGKSFSVKVNVTAFSCSRVQPE"
     gene            23657..24382
                     /locus_tag="PN977_002519"
     CDS             23657..24382
                     /locus_tag="PN977_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001696149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin co-regulated pilus biosynthesis Q family
                     protein"
                     /protein_id="HDI6042540.1"
                     /translation="MTPLKPLLIVPLLAFSVPLFAAPSPSQVSAPSSSTVSSAPGALK
                     FIPRDGKGLSLKKAMQQLIPPGWTFDIAPDVRPPRTVSWKGNDQWNYVTERMLKKYQL
                     RAITDTEKKHVRIERNTLFSPSQATPGTTSLQEKTAEKSANMPQPAHARTTWTLARGT
                     LLRAGLEQWVAAVPCQAGGTWQLAWQNPGLNYSIDAPLHFTGEFRHALSELFRLYQDA
                     QTPLYAAINSTQCVVHVSDRPGD"
     gene            24385..24822
                     /gene="pilM"
                     /locus_tag="PN977_002520"
     CDS             24385..24822
                     /gene="pilM"
                     /locus_tag="PN977_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000539880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus biogenesis protein PilM"
                     /protein_id="HDI6042541.1"
                     /translation="MGWSLSLVMALTVALTWFHNSSTHRVTVYSEQAEMRERAWEMVR
                     LMNGINDRLYTRPAERTGGGTLPVTATGFRPVYGTRHIIAGQRVFVWQDDRPGLAGAL
                     ADITHKTALAGRVKGRRLLNTTGQDTGISVPSVIPDSAFVYFN"
     gene            25012..26637
                     /locus_tag="PN977_002521"
     CDS             25012..26637
                     /locus_tag="PN977_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilN family type IVB pilus formation outer
                     membrane protein"
                     /protein_id="HDI6042542.1"
                     /translation="MKKRQHMLLPLTAVALLLSGCGTAERISNTEKDATATLEKARRH
                     AGELRKSRPVVTETTRQWINPSPVSVSPAHQSLPGCPIIINTRRDISLRQIAQRITGS
                     CGIPVTITADVWQALATTGPTQQLTGAIPAPDPNGMVPLAAVGSGAQVIPPGGGAVLP
                     ELSATGLRQLLDTVASRLGISYRYDSDRRAITFYWLETRTFPVTYMDSQVTYNARVVS
                     GTASSSSGSSTGSNAGTLSGEASNTQTTTVDMKSTLYEDLKNAVGAMLTPGTGRMVLA
                     TGFLTVTDTPRVLDTVSRFVEARNREMRRQVVLNVEVLSVRKTRRDQAGIDWNAVFSD
                     GRLGLTLGGAFSSAADDIITSGVSIVDGKLTGSKAFLKALSSQGTVSVVTQNSAVTKN
                     MTPVPMQVASQQGYIEGVTTDTTANVGTSTSLNAATITTGFNMTLLPFIQPDARSLQL
                     LFSMNLSDKPTFEVFESGGSRAQVPTVDLKTINQTIDLKSGQTVILSGFRQSGSKSVR
                     QGVGSPAFFGLGGGTDSGDDDTILVVLITPHVI"
     gene            26661..27949
                     /gene="pilO2"
                     /locus_tag="PN977_002522"
                     /pseudo
     CDS             26661..27949
                     /gene="pilO2"
                     /locus_tag="PN977_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001696151.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type 4b pilus protein PilO2"
     gene            27939..28400
                     /gene="pilP"
                     /locus_tag="PN977_002523"
     CDS             27939..28400
                     /gene="pilP"
                     /locus_tag="PN977_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000543769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus biogenesis protein PilP"
                     /protein_id="HDI6042543.1"
                     /translation="MHDKTKALLIAALLLPAMAVAGNENDASQNKQLQIEQIQEETAL
                     YEAMLAREKIRHELRTLEQGISPVSGSVTGHQPENAAGTSSGSQAFSSPRLKEIFGIQ
                     PQLSARVILPGGTETDVRAGQALPGTLFMVEQITPDSVILEYNGNRQTLRP"
     gene            28410..>28750
                     /locus_tag="PN977_002524"
     CDS             28410..>28750
                     /locus_tag="PN977_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001115301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion protein E"
                     /protein_id="HDI6042544.1"
                     /translation="MPETIELPPEDIRDFVAIQHDADGKIRVKVVEAKKHITSVQRYL
                     TLLRSRYPGKISKFDFTTLDVIATLNSNNTERAPGVLSDGGINTVQRKVLDYIRKAAQ
                     LDASDLHITPGR"
BASE COUNT         6733 a         8058 c         7239 g         6720 t
ORIGIN      
        1 tgctatgttg ctggaagcac agcgtcgtgg ttacgaactt cactatatgg agatgggcga
       61 tctgtatctg atcaatggtg aagcccgcgc ccatacccgc acgctgaacg tgaagcagaa
      121 ctacgaagag tggttttcgt tcgtcggtga acaggatctg ctgctggccg atctcgatgt
      181 gatcctgatg cgtaaagacc cgccgtttga taccgagttt atctacgcga cctatattct
      241 ggaacgtgcc gaagagaaag ggacgctgat cgttaacaag ccgcagagcc tgcgcgactg
      301 taacgagaaa ctgtttaccg cctggttctc tgacttaacg ccagaaacgc tggttacgcg
      361 caataaagcg cagctaaaag cgttctggga gaaacacagc gacatcattc ttaagccgct
      421 ggacggtatg ggcggcgcgt cgattttccg cgtgaaagaa ggcgatccaa acctcggcgt
      481 gattgccgaa accctgactg agcatggcac tcgctactgc atggcgcaaa attacctgcc
      541 agccattaaa gatggcgaca aacgcgtgct ggtggtggat ggcgagccgg taccgtactg
      601 cctggcgcgt attccgcagg ggggcgaaac ccgtggcaat ctggctgccg gtggtcgcgg
      661 tgaacctcgt ccgctgacgg aaagtgactg gaaaatcgcc cgtcagatcg ggccgacgct
      721 gaaagaaaaa gggctgattt ttgttggtct ggatatcatc ggcgaccgtc tgactgaaat
      781 taacgtcacc agcccaacct gtattcgtga gattgaagca gagtttccgg tgtcgatcac
      841 cggaatgtta atggatgcca tcgaagcacg tttacagcag cagtaaccca ccttagcgag
      901 aaggatctcg ttgagactct gagtgacagc gcccttcttt ccacgcatac tgggcgctgt
      961 tgcttttttg aaccaggaaa cagaacctct gacaatgaat ttacagcatc actttcttat
     1021 tgccatgcct gctctccagg atccgatttt ccgtcgttcc gtggtctaca tttgcgaaca
     1081 taataccaat ggtgcaatgg ggatcatcgt caacaagccg ctggaaaatc tcaaaattga
     1141 agggattctg gaaaagctga agatcacgcc ggagccgcgt gatgaatcaa tccgtctgga
     1201 taaaccggtt atgctcggcg gtccgctggc tgaagatcgc gggtttattt tgcatactcc
     1261 gccctccaat tttgcttcca gcattcgcat ttcagacaac acggtaatga ccacttcccg
     1321 cgatgtgctg gaaacgctcg gcaccgataa acaaccgtct gacgtattgg tggctctggg
     1381 ttatgcctcc tgggagaaag gtcaactgga acaagaaatt ctcgataacg cgtggctaac
     1441 ggccccggca gatctgaata ttctgttcaa aacgccgatt gccgaccgct ggcgcgaggc
     1501 ggcaaaactg attggtgtgg atattctcac catgcctggt gtggcaggac acgcctgatg
     1561 agtggaacct tactcgcctt cgacttcggc accaaaagca ttggcgtagc ggtcggccaa
     1621 cgcattaccg gcaccgctcg ccctttgcct gcaattaaag cacaggacgg tacgccggac
     1681 tggaacatta tcgagcgttt actgaaagag tggcagccgg acgaaatcat cgtcggtttg
     1741 ccgctgaata tggacggcac cgagcaacca ttgactgcca gagcgcgtaa atttgccaac
     1801 cgtattcatg gccgtttcgg tgttgaagta aagctccatg acgagcgtct tagcactgtg
     1861 gaagcccgtt ccggtctgtt tgaacagggc ggctatcggg cgctcaacaa aggcaaagtt
     1921 gactctgcct ctgcggttat tattctcgaa agctatttcg agcagggata ttaaggcgat
     1981 ttaaacgcct ggcggagtgt aaataatatc atcgcgtctt attgccggat gcggcgtgaa
     2041 caccttatcc agcacacatc tggcagcggc tataggtctg ataagacgcg ttagcgtcgc
     2101 atcagacatt tattgccttt gtaggcccga taagcttgcg catcgggcat ggcaacgtca
     2161 caaacgccct tcccccaccc ggtgctgata actctgctga aacgttatca tccccacctg
     2221 ctgcccggtt tgaataacat gcggtaactg gtgggttttc ccttcgcgaa tcaaattccc
     2281 caccgcgggt gtgttaatca gcaattcaaa tagcgccacg cgtccttcct gtttatccac
     2341 ttccagcttt tgtgacagca ctgcccgtaa actacctgcc agttgattac gcacggggtc
     2401 tttttcctgc gccggaaatg aatccaccag tcgctcaact gcctgcgcgg caccacgcgt
     2461 atgtaatgtt gccagcacca aatgcccggt ttctgccgcc gtcagtgcca gacggattgt
     2521 ctcgctgtca cgcagctctc cgagcaaaat cacatcagga tcttcccgca atgcggcccg
     2581 caatcccgat gcgaacgtca tacagtgcaa accaatttcc cgctgctgga tcaaacatcg
     2641 ctggctggca tagagatatt caacaggatc ttccagcgtc agaatatgcg catcggcatg
     2701 ttgattgaga tagccaacca tcgccgccag cgtggtagat ttgccactcc ccgtcgcccc
     2761 cgtcaccaga atcaggccat tctcgctctt gagtaattcc ggcaataccg ttggtgcgcc
     2821 aagctgttcg agctgcgggc agtgcgaagg taacagccgt aacgccagcg aaatgccatg
     2881 ccgttgcgcg aatgcgctgc cacgcaatcg ctggttttcc gccagcgaca cggcaaaatc
     2941 cagctgacca ttctccagca atattgcccg ctgatcgtca tccagccact cccgcagtag
     3001 ctcttcgacg tccggcgtat caaacggcgc agcttccatt ctgccgcgaa tgcgccatcg
     3061 tgcgggccag gcgctgcaca ggtgtagatc cgagacgtta tgctttacac taagggccac
     3121 aatttcttcc atattcatac taagatcctc ggaaaatgaa cgatattgcg cataacctgg
     3181 cacaggtccg ggacaaaatc tcagcggctg caacgcgttg cggccgttct ccagaagaaa
     3241 ttacgctgct tgcagtcagt aaaacaaaac ctgcgagcgc catcgcagaa ggcattgatg
     3301 ccgggcagcg tcaatttggt gaaaactacg ttcaggaagg ggtagataaa attcgccact
     3361 ttcaggaact gggcgtaaca ggattagaat ggcattttat tggcccgttg cagtctaata
     3421 aaagccgcct ggtggcagag catttcgact ggtgtcatac catcgaccgt ttgcgcatcg
     3481 ctacccgtct caacgaccag cgcccggcag aacttccccc tcttaacgtt ctgattcaaa
     3541 ttaacattag tgatgaaaac agtaagtccg ggattcaact ggctgaactg gacgagctgg
     3601 cagctgcggt cgctgaacta ccgcgtttac gtctgcgcgg gttgatggca atccctgcgc
     3661 ctgagtcaga atatgtaagg cagtttgaag ttgcacgcca aatggctgta gcatttgccg
     3721 gactgaaaac gcgctacccg catatcgaca cgctctctct gggaatgtcg gacgatatgg
     3781 aagccgccat tgcggcaggt agcacgatgg ttcgtatcgg cactgcaatt tttggtgcgc
     3841 gtgattactc taaaaataag gaattaaagg aacgccatga atacgttgac tttcctgctt
     3901 tcaacggtca ttgagctgta taccatggtg ctgttattac gcatctggat gcagtgggct
     3961 cattgtgatt tttacaaccc cttctcacag tttgtagtga aggtaacgca gccaattatc
     4021 gggccactgc gccgcgttat tccggcaatg gggccaattg acagcgcctc gctgctggtt
     4081 gcctatattc tcagttttat caaagccatc gtgctgttta aagtggtgac cttcctgcca
     4141 atcatctgga ttgccggttt actgattctg ctgaaaacca tcggcctgct gattttctgg
     4201 gtcctgctgg tgatggcgat tatgagctgg gtaagccagg ggcgtagccc gattgaatac
     4261 gtgctgattc agctggccga tccgctgctg cgcccgattc gccgcctgct accggcaatg
     4321 ggtgggattg atttctcgcc gatgatcctc gttctgctgc tgtatgtcat caatatgggt
     4381 gtcgcagaag tattacaggc aaccggaaat atgctgctgc cagggctgtg gatggcgtta
     4441 tgaatgccgt aacagttaat gatgacggtc tggttttacg gctctatatt cagccgaaag
     4501 ccagccgtga ttctattgtc ggtttacatg gcgacgaagt taaagtcgcc attaccgcgc
     4561 cgccggttga cggccaggcc aacagtcatc tggtgaagtt tctcggtaag caattccggg
     4621 ttgccaaaag ccaggtggtg attgaaaaag gcgaacttgg ccgccacaaa caaattaaaa
     4681 tcattaatcc gcaacaaatc ccgccagaaa tcgcggcgtt aattaattag gtatcctatg
     4741 caaaaagttg tcctcgcaac cggcaatgtc ggtaaagtgc gtgagctggc gtcgctgctt
     4801 agcgacttcg gtcttgatat cgtggcccaa acagacctcg gcgttgattc cgctgaagaa
     4861 accggcctga cctttatcga aaacgcgatt ctgaaagcgc gccatgcggc aaaagtgacc
     4921 gctttaccgg caattgccga cgactctggt ctggcggtag atgtgcttgg cggcgcgccg
     4981 ggcatttact ccgcgcgtta ttccggtgaa gacgcgaccg atcaaaagaa tctgcaaaaa
     5041 ctgctggaaa caatgaaaga cgtaccggac gaccaacgtc aggcgcgttt ccactgcgtg
     5101 ctggtatatc tgcgtcacgc ggaagatccc actccgctgg tgtgccacgg cagctggccg
     5161 ggcgtgatta ctcgtgaacc agcgggcact ggtggctttg gttatgatcc aatcttcttc
     5221 gtaccttccg aagggaaaac tgctgccgaa ctgacccgcg aagaaaagag cgccatttcc
     5281 caccgtggtc aggcgttgaa actgctgctg gacgctttac gtaatggtta aattaccgcc
     5341 gctgagtctc tacattcaca tcccgtggtg cgtgcagaaa tgcccgtact gcgatttcaa
     5401 ctctcacgcg ttgaaaggag aagtgccgca cgacgattat gttcagcatc tgcttaacga
     5461 tctggacaac gatgtggctt acgctcaggg ccgtgaagta aagacaatct ttattggcgg
     5521 tggtacgccg agcctgcttt ccggcccggc gatgcaaacg ctgctgaacg gcgtacgtgc
     5581 gcgtttgccg ctggcagcgg atgcagaaat tacgatggaa gcgaaccctg gtacggttga
     5641 agccgatcgc tttgttgatt atcagcgtgc tggtgtgaac cgcatctcta ttggtgtgca
     5701 gagttttagc gaagaaaagc tgaaacgact tgggcgtatt catggcccgc aagaagcgaa
     5761 acgcgcggcg aagctggcga gcggtttagg gttacgtagc tttaaccttg atttgatgca
     5821 tgggctgccg gatcaatcac tggaagaggc gcttggcgat ctacgccagg ccattgaact
     5881 gaatccgccg catctttcct ggtatcaact gaccatcgaa cccaatacgc tgtttggttc
     5941 gcgaccaccg gtgctgccgg acgatgacgc gttgtgggat atattcgaac aggggcatca
     6001 gttattaacc gcagcgggtt atcagcaata tgaaacttcc gcttacgcca aacccggtta
     6061 tcagtgccag cacaatctca actactggcg ctttggtgac tacatcggta ttggctgcgg
     6121 cgcacacggc aaagtgacct tcccggatgg gcgcattctg cgtaccacca aaacgcgtca
     6181 tccgcgtggt tttatgcaag gaaggtatct ggaaagccag cgtgatgtcg aagccacaga
     6241 taagccgttt gagttcttta tgaatcgctt ccgtctgctg gaggccgcgc cgcgcgtgga
     6301 gtttattgcg tataccgggc tttgcgaaga tgtgattcgc ccacagttag acgaggcgat
     6361 tgcccagggt tatctcaccg aatgtgcgga ttactggcag ataacggaac atgggaagct
     6421 gtttttaaat tcgctgctgg agctttttct ggctgagtaa acttgtattg ccggatgcgg
     6481 cgtgaacgcc ttatccagcc gacatgtggc agcggttgta ggtctgataa gacgcgcaag
     6541 cgtcgcatca gacgttgatt gccggatgcg gggtcaacgc cgcatcctgc tacaaatcgt
     6601 gcactatatc aaacttactt aatcacccca ttgcgccgca gttgttcggt atcttttttc
     6661 cagcgttcat tagcttggtt gatatcgtca attttcatca aaatcgcgac ttttttactg
     6721 tttatttctg aaactttcat cgttaacgga tattgccctt tttcgagcgt tacccagtca
     6781 tcctgcaatt tacggctaag tttttgtgat tgcagaaaag tctgtcgccg ttgttcgttg
     6841 atatcgaaat ctcgtttctg ctgaatcggc acttcataac actcttcact ctgtttatga
     6901 catgccgcaa tcgccggttg ataaacctct ttgtcatatt taaccgcgaa cgcctcgcta
     6961 tcgttctgtt tattaaactc atcacgctgt tggtgaattt ttttaaaggc gtcttcgcgg
     7021 gtcatttgct taccgtttgc atcttttccc caatatgcat ccgctgcttt ctgcgccgcc
     7081 accatttgct tatcaagcgc tttcacttcc ttttccagcg caggaatcga gttcttctgg
     7141 gtattcaacg cagattcaag ctgactaaag cgataattga aatcttcgct attcacgatc
     7201 agatatttat tattcgtttt gatatcgtcg accacccgcc cacgatcgct ggctgccgcc
     7261 tgaaaagagt aaaagttcac cgaccagcca gacgcgggcg ttcctttact ggttaacatc
     7321 gccgagaatt ttaccggttt atctttcgtc caggtctgtt cgagcagttc gtaatctgcc
     7381 aactgaccga cccaggtata gagatcgtca ctggaagaga catcgccttc cgctgaccag
     7441 gtggcctgat tacccttagc atcaagattt tttaaggtga tagcatcaag cttgagaatg
     7501 ccgtgatact gctttttgaa ttgatctttt aagatatttt cagttggcgg ttcgccatct
     7561 gcccaggcat taccagtcat caacataagc agtgccgtgc caacaatcca ttgctttttc
     7621 acccgaaaaa cctcacgcgg aataatttat tccggtgatt atcattaggg taattaaaat
     7681 tggcaatgga cgtgagtctg aagtgaaaaa gccccggcac gataccgggg cgaggcgatt
     7741 agtactgatt gaagatctgc tggatctgct gcggatcttt ggtttgcgtc agagccagtt
     7801 gcagcagaac gcgcgctttt tgcgggttca gcgtgccaga ggcgacgaag ccgtatttcg
     7861 catcatccac ttcggcatcc tgagtggtag cgcccgtcgg tacgcgggaa gaacgcacga
     7921 ctgcagtacc ggttttcgcg gcggtcgcca gcgtgtcgaa cacagattta tacaggttgc
     7981 cgttacccac accagcgcta acgatgccat catagcccgc atctaccagt gctttagccg
     8041 gaagatcgga tgcgttagcg tagttataaa caatgccgac tttcggcagt tcattcagct
     8101 tagagacatc gaatggcgtg tcgctggtat gcttacgtgc cggggtacgc tggtagtcaa
     8161 tcttaccgtt gtgaatgtaa cccagaggac cgtagttaac agacttgaag gtcgctacgt
     8221 cggtggtgtt ggttttggtg acgtcacggc catcaagcac ggtgtcattc atcactacca
     8281 gcacgccacg gttggcggag gctttatcag ctgcggtcac taccgcgtta tacaggttga
     8341 atggaccgtc tgcgctcata gacgtggacg gacgcattgc gccgaccatc accaccggtt
     8401 tgtcgcattt caccgtcagg tcgaggaagt aagcagtttc ttccatcgtg tcggtaccgt
     8461 gggtaatgac gaagccgtcg gtcttatcgc agtcggtgtt aatttttttc gccagtgtca
     8521 gccagacatt atcgttcatg tcctgggagc cgatattcac tacctgctcg cctttaacgt
     8581 tcgcaatgtc ttttagttgc ggcaccgcat taaccagatt ttctacgcca actttacccg
     8641 ctgtgtagtt agatttggtt gcggagtcac caccaccggc aatggtcccg ccggttgcta
     8701 aaatggtgat attgggtaat gccaatgctg caccactaaa acccataacc agtgcggcaa
     8761 gtgccgtctt tttgaaaaac tccatttcat tcctccagtt acgtgaacgc tacgcattat
     8821 cccttagctc tgtatgggaa atttgacgtt aaacaattta caacgtgaat atattttgga
     8881 gatctacaaa gttagaggca ggtaacaaaa cgaagaatta aacggcataa aaaagtatta
     8941 tgccgtctta aaatagagga ttattttaaa ttcccgacca gggctttgcg gctatcttcc
     9001 agagtcacaa cgcggctaca aacatctttg ccaaactgct ggaaatcttt ttcctgcttt
     9061 ttccactcgg tttggattga ggattgcagc ccccccaggc ttcccagcac attctgtaat
     9121 gggttaccgc cgcttttcag caccgctttc gcgcccattt cattaatgct gtcctgtaaa
     9181 attccaccca ttgcctgatt cactaattgc tggccttcgg cgcgaacctg atcaatggct
     9241 ttatagtgaa acgtcaggcc atcgctgcgc gtttcaataa tgcggttcat ctgctctttc
     9301 agctgcgcat caagtttggt cagacggctg cgcattttgc tgctttcgcc catctcctga
     9361 acgataattt tatccagagc aatacgggct ttctcgacgc ggcttttcgc gccttcatca
     9421 atccacggca gcgtgctgcg tagttcagcc tgataatcct tcgcctgctc gcgctgggcg
     9481 gcattcaggg aatattgctt accgttatac atcacgttgc cgtctggcgt gatcaccaga
     9541 ttgccgtttt cgcccttcac ctgcacggtt tgcgggctga caatcacatc gtcacgcggc
     9601 gtgacgctgc actggtagtc ggcgtgagcg gtcattgccg tcactgaaag tgctgccgcc
     9661 agcagcattt tgcgcatcat agtcttccct caagaaaaaa tcaggccagc atttgctggc
     9721 cccagattga taacaaagta cgcgttttcc atgccggatg cggcgtaaac accttatccc
     9781 gcctactcga tcgtgcatct ttccaggcct gataagcgta gcgcatcagg cagttttgca
     9841 tttgtcatcg cccgtatgct ttcttagtcc caccaaacgt cgaaaagttc gctggtgcgt
     9901 acctcatcca gtttgcgctc ttccagccac ttacgcacaa tcgcctgatg ttcttcggtg
     9961 catttgccga tttcctgcat gcagatcaga ccttcccagg ccagataacc gctgccgtca
    10021 aaggccagtt tgttcggttc gataacctcg ttaataaaat catcaacggt tttatcaatc
    10081 tgttcttccg atgtaccttc cgggaatcgc catgccaccg aaaatcctaa ttcctggaat
    10141 tcgtcgatgt gcattttttt acgcagacga cggctacggt tctttgccat tatttcaccc
    10201 tctcgaacat taagtcccat actccgtgac caagacgatg accacgttgt tcaaatttcg
    10261 tcaccggacg tgatgccgga cgcggtacgt aatcattgct ctctgacagg tttttataac
    10321 cgtcaataga agacatcact tcaagcatat gttccgcata aggttcccag tcggtcgcca
    10381 tatggaatac gccccccagc tgcagtttgc tttttaccag ttcggcaaac ggcacctgaa
    10441 cgatacggcg tttattatgg cgcgctttgt gccacgggtc agggaaaaag agctgcacca
    10501 tgcgcaatga attgtcagga atcattttat gcagcacttc aaccgcatcg tgacacatca
    10561 cgcgcaggtt gcttaaacct tcttcatgcg cagaagccag gcacgcacca acgcccggtg
    10621 aatgcacttc aatgccgagg aagtcctgct cagggcgatc tttagccatt gccaccagcg
    10681 acgcccccat gccaaaacca atctcaagcg tcaccggcgc ttcacggcca aaaagcgcgg
    10741 ggaaatccag catatcttcg ctgaactcaa cgcccatcac cggccagtag ttttccagcg
    10801 catgttcctg gcctttggtc agtcgcccct ggcggcgcac aaaactacgg atacggcgca
    10861 gtgggcggcc gttttcatca aattccggtg aaatgacgtc gtttttcata aaggtttagt
    10921 cgcttgtgaa agtgttctga aaacgggcat tatccaaagt tagttgccgg atgcaagcat
    10981 gataaggccg tggctgcgga aagttccggt ttacaccctg ccgtcgctgt gctgcaatct
    11041 tgcccccaac aacagtgaat tcggtgacca tgcaagcgtc gcaattttca gcccaggttc
    11101 tggactggta cgataaatac gggcgaaaaa ctctgccctg gcaaattgac aagacgccct
    11161 acaaagtatg gctctcagaa gtgatgttgc aacaaactca ggttgcgacc gttatcccct
    11221 attttgaacg ctttatggcg cgcttcccga cggtgaccga tctcgccaat gcgccgctcg
    11281 acgaagttct ccacttgtgg accgggcttg gctattacgc ccgcgcgcgc aatctgcata
    11341 aagcggcaca acaagtggcg accttacacg gcggtaaatt cccggaaacc tttgaggaag
    11401 ttgcagcact gccgggcgtc gggcgttcca ccgcaggcgc gattctctcg ctttctctgg
    11461 gtaagcactt tccgattctc gacggtaacg tcaaacgcgt gctggcgcgc tgctatgctg
    11521 taagcggctg gcctgggaaa aaagaggtcg agaataaatt atggagtttg agcgagcagg
    11581 tgacgcccgc ggttggcgtg gaacggttta atcaggcgat gatggatttg ggtgcgatga
    11641 tttgtacgcg ctcgaaaccg aaatgttcgc tctgtccgct acaaaacgga tgtattgccg
    11701 ccgccaacaa tagctgggcg ctttatccgg gcaaaaaacc gaaacagacg ctgccggagc
    11761 gcaccggcta ctttttgcta ttacagcacg aagatgaagt attgctggcg cagcgtccgc
    11821 cgagcggatt gtggggcggt ttatactgtt tcccgcagtt tgccgacgaa gaaagtttgc
    11881 ggcagtggct ggcgcaacgg cagattgctg ccgataacct gacgcaactg accgcgtttc
    11941 ggcatacctt cagccatttc cacttagata ttgtgcctat gtggcttccc gtgtcgtcat
    12001 tcaccggctg catggatgaa ggcaatgcgc tctggtataa cttagcgcaa ccgccgtcag
    12061 ttggcctggc ggctcccgtg gagcgtttgt tacagcagtt acgcactggc gcgccggttt
    12121 agcgcgtgag tcgataaaga ggatgattta tgagcagaac gattttttgt actttcctgc
    12181 aacgtgaagc agaaggtcag gattttcagc tgtaccccgg cgagctggga aaacgcatct
    12241 ataacgagat ctccaaagaa gcctgggcgc agtggcagca caagcaaacc atgctgatta
    12301 atgaaaagaa actcaacatg atgaatgccg agcaccgcaa gctgcttgag caggagatgg
    12361 tcaacttcct gttcgagggt aaagaggtgc atatcgaggg ctatacgccg gaagataaaa
    12421 aataaaaaca gtgccggagc acgcctccgg caacttgcat aaaaacaaac acaacacgca
    12481 cccggaatga tgaaaaaata tctcgcgctg gctttgattg cgccgttgct catctcctgt
    12541 tcgacgacca aaaaaggcga tacctataac gaagcctggg tcaaagatac caacggtttt
    12601 gatattctga tggggcaatt tgcccacaat attgagaaca tctggggctt caaagaggtg
    12661 gtgatcgctg gtcctaagga ctacgtgaaa tacaccgatc aatatcagac ccgcagccac
    12721 atcaacttcg atgacggtac gattactatc gaaaccatcg ccgggacaga acctgccgcg
    12781 catttgcgcc gggcaattat caaaacgtta ttgatgggtg acgatccgag ttcggtcgat
    12841 ctctattccg acgttgatga tattacgatt tcgaaagaac ctttccttta cggtcaggtg
    12901 gtggacaaca ccgggcagcc gattcgctgg gaaggtcgcg caagcaactt cgcggattat
    12961 ctgctgaaaa accgtctgaa gagccgcagc aacgggctgc gtatcatcta cagcgtcacc
    13021 attaacatgg tgccgaacca ccttgataaa cgtgcgcaca aatatctcgg catggtccgc
    13081 caggcgtcac ggaaatatgg cgttgatgag tcgctgattc tggcaattat gcagaccgaa
    13141 tcttccttta acccgtatgc ggtcagccgt tccgatgcgc tgggattaat gcaggtggta
    13201 caacatactg ccgggaaaga tgtgttccgc tcgcagggga aatccggcac gccgagccgc
    13261 agtttcttgt ttgatcctgc cagcaatatt gataccggca ccgcgtatct ggcgatgctg
    13321 aacaatgttt atctcggcgg aattgataac ccaacatcgc ggcgttatgc cgtcatcacc
    13381 gcctataacg gcggcgcagg cagcgtgctg cgagtctttt cgaatgataa gattcaggct
    13441 gccaatatta ttaacaccat gacgccgggc gatgtttatc agacgctgac gacccgccat
    13501 ccctctgcgg aatctcgccg ttatctttat aaagtgaata ccgcgcaaaa atcctaccgc
    13561 cgccgataat tccattaacc gcccctgacg atgctcaggg gcaaaaatgt tatccacatc
    13621 acaatttcgt tttgcaaatt gggaatgttt gcaattattt gccacaggta acaaaaaacc
    13681 agtccgcgaa gttgatagaa tcccatcatc tcgcacggtc aaatgtgctt tttcaaacac
    13741 tcatccgcat cacgatgtga ggaaattaac atgaatctta agctgcagct gaaaatcctc
    13801 tcttttctgc agttctgtct gtggggaagt tggctgacga ccctcggctc ctatatgttt
    13861 gttaccctga agtttgacgg tgcttctatt ggcgcagttt atagctcact gggtatcgca
    13921 gcggtcttta tgcctgcgct gctggggatt gtggccgaca aatggttaag tgcgaaatgg
    13981 gtatatgcca tttgccacac cattggcgct atcacgctgt tcatggcggc acaggtcacg
    14041 acaccggaag cgatgttcct tgtgatattg attaactcgt ttgcttatat gccaacgctt
    14101 gggttaatca acaccatctc ttactatcgc ctgcaaaatg ccgggatgga tatcgttact
    14161 gacttcccgc caatccgtat ctggggcacc atcggcttta tcatggcaat gtgggtggtg
    14221 agcctgtctg gcttcgaatt aagccacatg cagctgtata ttggcgcagc actttccgcc
    14281 attctggttc tgtttaccct gactctgccg catattccgg ttgctaaaca gcaagcgaat
    14341 cagagctgga caaccctgct gggcctcgat gcattcgcgc tgtttaaaaa caagcgtatg
    14401 gcaatcttct ttatcttctc aatgctgctg ggcgcggaac tgcagattac caacatgttc
    14461 ggtaatacct tcctgcacag cttcgacaaa gatccgatgt ttgccagcag ctttattgtg
    14521 cagcatgcgt caatcatcat gtcgatttcg cagatctctg aaaccctgtt cattctgacc
    14581 atcccgttct tcttaagccg ctacggtatt aagaacgtaa tgatgatcag tattgtggcg
    14641 tggatcctgc gttttgcgct gtttgcttac ggcgacccga ctccgttcgg tactgtactg
    14701 ctggtactgt cgatgatcgt ttacggttgc gcattcgact tcttcaacat ctctggttcg
    14761 gtgtttgtcg aaaaagaagt tagcccggca attcgcgcca gtgcacaagg gatgttcctg
    14821 atgatgacta acggcttcgg ctgtatcctc ggcggcatcg tgagcggtaa agttgttgag
    14881 atgtacaccc aaaacggcat taccgactgg cagaccgtat ggttgatttt cgctggttac
    14941 tccgtggttc tggccttcgc gttcatggcg atgttcaaat ataaacacgt tcgtgtcccg
    15001 acaggcacac agacggttag ccactaatta cgcaaagaaa aacgggtcgc cagaaggtga
    15061 cccgtttttt ttattcttac ttcaacacat aaccgtacaa ccgtttcacg ccatccgcat
    15121 cggtttcgct ataaacacct tgcagctccg gcgaaaatcc cggcaacaaa ttcccccctt
    15181 cttccagtgc aaggaaataa cgttgaaccg ccccacccca gacttccccg ggtaccacgc
    15241 aaagcacgcc aggtggataa ggcaacgccc cttctgccgc aattcgccct tcggcatcac
    15301 gaatccgcac caactccacg tcaccgcgaa tataagcgct atgcgcatcc tgggggttca
    15361 tcaccactga cgggaaactc tgctggcgga acatcgcttt ttgtaggtct ttgacgtcga
    15421 aactgacata cagatcgtgc atctcctgac acaactggcg cagggtgtag tcgcgatagc
    15481 gcaccggata cttgttataa acgctcggca acacctcaac cagcggcgag tcatcctcaa
    15541 tatgctgttc aaattgcgcc agcatcgcca ccagttgtgc cagcttctcg tggctttccg
    15601 ccggagttaa taaaaacaga atggagttga gatcgcactt ctccggcaca atgccgttct
    15661 cacgcagata gtgcgccaga atcgtcgccg gaacgccaaa gtcgctatat tcgccggttt
    15721 cggcatcgat acctggtgta gtgagtaaca gcttgcacgg atcaacaaaa tactgatccg
    15781 cggcatatcc ttcaaagccg tgccacttcg cccccggctc aaaactgaaa aaacggcggt
    15841 cgctggctaa cactgatgtc ggataatcct gccacaattt gccatcaaca acgggcggga
    15901 taaacgggcg gaacagctta cagcgcgcaa gaatagcctt gcgcgcttca atccctatct
    15961 caacacactc agcccacagc cgacgcccac tctccccttc atgaattttg gcgttaacat
    16021 ccagtgcagc aaacagcgga tagaaagggc tggtagaagc atggagcata aaggcgttat
    16081 tcaaccgctt atgcgggcaa aaacgcgcct gtccgcggat atggttatct tttttatgga
    16141 tctgcgacgt ctgtgagaat cccgcctgct gtttgtgcac cgactgagtc acaaagatcc
    16201 ccggatcgtt ttcgttaagt tctaacagca gcggcgagct atccgccatc atcgggataa
    16261 attgttcata accgacccac gcggaatcaa acagaatgta atcacacaga tgcccaacgg
    16321 tatcgatcac ctgacgggcg ttatagacag tgccgtcata ggttcccagc tgaataatcg
    16381 ccaggcgata cgggcgcggc aggtcggctt tttctggcgc aacgtcgcga atttgctggc
    16441 gcagatactc ttcattaaaa cagtgcgcat caataccgcc aatgaaacca aacgggttgc
    16501 gtgaagcttc cagatagacc ggcgtcgccc ccgcctgaat cagcgcgccg tgatgattcg
    16561 acttatggtt gttacggtcg aagagcacca gatcgccacg cgttaacagc gcattcgtca
    16621 ccactttatt cgctgccgat gtgccgttca gcacaaaata ggttttatcg gcatgaaaga
    16681 ctttggctgc gaatttctgc gcatctttcg ccgatccttc atgaataagc agatcgccca
    16741 attttacgtc agcgttacac atatcggcgc gaaagacgtt ctcaccaaag aaatcgtaaa
    16801 aatggcgtcc ggcaggatgc tttttaaaaa acgcaccatg ttgatgtcca gggcaagcaa
    16861 aggtgctgtt gcccatctca acgtactgcg tcagcgtgtc ataaaacggt ggcagcaaat
    16921 tctcttcata ctgacaggct gcggattcca gctccagcca ctgctgctcg ttgccgttga
    16981 ttaccgccgt aacgcccgca ggtaattcaa cagcatgttc ggaatacaaa aacaccggta
    17041 gatgaaaacc ggtgcgctta agcaacgcaa gaatgccact gcgactatcc gcagcggtaa
    17101 tgacgactgc cgcgacgtcc gtaaaatcag tatctcccaa cgccaccacg cggcgatgag
    17161 tagaaagtcg ggataccagt tcactactgg cggcaatatt cattgatttc ataagcgcaa
    17221 aacccgtttc ggggaagtaa gaataccgga caaggtgaaa aaccctgccc cacgagatat
    17281 gggtcaaact ggtcaccagc tccgaccgcc agacatcagt aaaagcagaa acgctctgat
    17341 tttactgttg tcctgcagtg agcgtgcgtt aacctcaccg catgagcaga aaaagggagg
    17401 agtttcgcgc gaaacagcga taagcgagag aatgtaagga ggtggcgtgc atcgggcaaa
    17461 ctccgtacag agaggagaaa attcgcgcaa tcatggcacc tttcaccaga gcgtgcaagc
    17521 caggactttg cgaacaaaca agccatcgaa ggagccaaac ggtcataata agaaaatcag
    17581 acaatgcagc taacaggaac tcttgtggtc atcggtcctt ttatcaacgc aagtgcagta
    17641 ttactgggtg gcgttctcgg tgcactcctt agtcaacgct taccggaacg tatccgcgtc
    17701 tccatgacat caatttttgg tctggcatcg ctggggattg gtattttact ggtggtgaaa
    17761 tgcgccaacc ttccggcgat ggttttagca accttactcg gtgcattaat cggcgaaatg
    17821 tgtctgctgg aaaaaggcgt caataccgcg gtcaccaaag cacaaaatct gtttcgccac
    17881 tcacgtaaga agccagcgca tgaatctttt attcagaatt atgtcgcgat tattgtcctg
    17941 ttttgcgcca gcggcaccgg aatcttcggg gcgatgaacg aagggatgac cggcgatccg
    18001 agtattttaa tcgccaagtc atttctcgat ttctttacgg cgatgatctt cgcctgctcg
    18061 ttgggtattg cggtatcggt gattagtatc ccattactga tcatccagtt aacgctggcg
    18121 tgggctgccg cgctgatatt accgctgacc acaccgtcga tgatggcaga cttcagcgct
    18181 gtaggcggtt tattgctgct ggcaaccgga ttacgcatct gcggcattaa aatgttcccg
    18241 gtggtcaaca tgcttccagc actccttctg gcaatgccgc tttccgccgc ctggaccgcc
    18301 tggtttgcct gacaatgcgt gcaatagcgg caaagtgatg atagattgtg cagtctgcag
    18361 taaattgaag aaatttgatt gacgagacga ggcgaatcag gtttaatgcg ccccgttgcc
    18421 cggatagctc agtcggtaga gcaggggatt gaaaatcccc gtgtcggtgg ttcgattccg
    18481 cctccgggca ccaccttaca tcaaagccac tggtcaggag tgagaaccct gacgggtgtc
    18541 atcgggaagg tgcaagatgt cgtgagtcta ggtcatgcca tctcattttc tcgattgggg
    18601 agcatgttgg tcgcatgctc cctttctcga tcattcatta tgattgcaac ggtttttcat
    18661 gttgtcaaca actgtaagca atcacctgat taaagatcgt ctgtcagttt tgacagttcg
    18721 gcctcaagta tacgtgctgt tcccttcaga tccatctcca catattccag tgtcgttgaa
    18781 acattccggt gacccagcat ggttttcacc atgtacagat ttcgttccgg tgccgccatc
    18841 agagtacttg ccagagtgtg tctgaaacga tgtgcagata cctgaaaacc acactctttc
    18901 gacagacgcc ggaaaaaacc tttgaccttc tgctcttccg gaggtccgct caccgataat
    18961 gcttcagaca caggcaaagc atagcgatga tagtggaaaa gataatcatc aggcgtggct
    19021 ccacgtttca ccgcttcatc catcagcctg gttatccccg gaaccagatg actgaccatc
    19081 gggatccgcc actcatgatg tgtcttgctg ccttcagagc ataaatcaat ccaccgttct
    19141 tcaggattaa catcccgcag gcgcagatgc agcagctgat tcagcctcat ccccgtaaag
    19201 cgaagtgtgt taaccacaat aatccagtac caggccggac gaatggcgca cttccagtgc
    19261 atcttcaggt tcatttcatc ctcctgaaac tgctcaagga ccagcaggat gcgttttatt
    19321 tgtttccggg tcagtgtttt tttgcgtttc ttttcctgtc tgacgctcat accctcaaac
    19381 gggttacaac ttaaatgcag gagttcagat gaggaagcaa aactgaagat agcgcgcata
    19441 tggcgtacct tgttgttcca tgtatgagtt gaacgtttct ggtttttcag gacctcccgt
    19501 cgccactccc tgacatgttg ttgtcggata tcttccggta acagagtccc gccaacaaac
    19561 ttacggaatc cgttcagcac ctttctgtag ctccactccg tagcttcacg tactgaacgg
    19621 cagaaaaaaa actcatccat caaatcattc cagcacttat ccgcattatt tattttcatt
    19681 gattaccttt tacatttcat tcacattcct gcaataatag cctcccctcc actcacgaaa
    19741 taaaaagata aggactgtct ttcagtttac tttcatgcat cagactacgt cctttgacca
    19801 ggtaaccttt gactgttttg taacgtgcac agttcccggg ttcctcagca atacgagcaa
    19861 aataaaaacg ctggtcatca cggcgtttat tgatacagag ccgctcaaat gatgactgaa
    19921 caatcgggcg tttgtccccg ggaagctgca ctgctttcag gtaatcaaag aaaatgcctg
    19981 gtaccggcag gaaaaccagt cctgcaacaa tgtgtatttt gtcgcctttc tggttaaatg
    20041 atatctcacc actgttcagc cctgctttaa gccagtccaa aaacgtctct ccctttaaat
    20101 caggatcatc tccggtaagc ggattcactt ctgttgcgcg gtattccccg gatacggact
    20161 gattaaccga cgcgtcactc tccaccagat acggcgattc atgaatactg cccggagacg
    20221 aatcgccttc atccggtcca tcggtgagat cctgcgcttc ttcctcatca gccagcatca
    20281 ccacatcccc attatcccgt tcagcagttt cggactcatc tgctgaaaaa agactcagca
    20341 gcattgctgt gtcctgatcc gcaggagaag cactgaccgc ttcatcatct gccgtcacca
    20401 ctggcgctgt ctgaaccgtc agttcctgct gtgcggacgg cacctctgac gctccggcgg
    20461 actcaccacc accttcagtc tctgtctgca cgctctcagt cttcacctcc tctgccggct
    20521 gcacctcagt gactgccatc acactgtccg tgctgccggt caccaccggg tcagcaggac
    20581 ggatgtctgc tgaagcctcc gcagtaaccg gcgcagtgac agcgcaggta ttcagcggac
    20641 tctctgtctg ccttgccgct tcctgcagca catcacgaat cagcgccatc ccgggatgct
    20701 gatgccagag cgccccggcc agattctgca gcgcccccgg cgccgtcaca agccaggccg
    20761 ttgcgccgtc cggtaacagc gtgccggcag tcagggccgc cagttgttgc gcttccgtgc
    20821 cctgcggcgc cgtcttcctg aaccggaaac ggaaaggttc gtcaggcaca gaaatccctg
    20881 gctgccagcc ccgaccactc agcagctccc cctcaaggtg cgccagcagg ggcaggtggt
    20941 aaaacagcgc tgaccagaaa atcaccgtat gccagactgc ggcctgagcc gcctggtctt
    21001 ccggggtcgc atccggtgga aacatgtacc ctcttgccag acgaacggca tacgtggtgt
    21061 acagtaccgt cagatcacca aatccaccgg cacgggacca ccgtccttcc cgtgttgccg
    21121 gcacattctg ggtgttcagc aacagccggc tgagtggcgc aaggcacatc ctgtcgtata
    21181 catcacgggg catcggattg ttatcccaga cctgcctgag cctcagctcc ctgtgctctg
    21241 gtgataacag tgcctctgcc gtgagcggcg cataaaaccc tgccggtatc accggcgcga
    21301 ccacgccagg ctccgtgccc tttgtgccgg gcagcatacg tctgaaccgc ttaaacatcc
    21361 tctccctcca caccatcgtc ttccttcccc tgcaccggcg cgtttccggg tgcaccacca
    21421 tcgccggcag cttccgtcag ctgctgcatg agttcattga gaaccagcgt tctgcgcgcc
    21481 cgggtgaccg ccacatagag cagattggtt tcatcccgcc gctcctgctc gcttatcagc
    21541 gggtcggtga tatcctgaaa atcatcatcg agtaccacca gtgaccactc cagtccctta
    21601 ctgcggtgtg ccgttgagac cgtgatatcc gcctcctgtt cattcgtgac ggtgtgcttt
    21661 ctgagaagtt cgagtttctt cggcagcgga aagcacagct ccagcagacg aatggactga
    21721 ttcatttcca catccttcgt caccttcgca atatgacggt actcgtcaaa accccggtag
    21781 tccttccgca gtctgtcact gtgcatccgc tccggcatat ccatctgaaa ccagtaaaga
    21841 tcttccagtg cctccgttct gtagccttca atgcccccca cccagtaaac cttcctgcca
    21901 cgcattgccg ccatcagtgc ggtgccaatc acaccggcca ccgtccggct cagtatcgcc
    21961 ctgtgaccgg tcatcttccc tgccttcacg cgcgcacgca tttcacctgc cgtcagcact
    22021 tcatcatccc cgcccctgcc ggtcacctca cggctctcgc cggcacgctt cagtaactgg
    22081 ctggccaccc cggccacggc aggaccaaaa cgaaaacttt gcgtcagcca gagacggtcc
    22141 gccccgttca gtgccggatg cgacagtgca ttgctggcac cacgaaaacg ataaatctgc
    22201 tggtaacggt cgccaaccag taccaccctg caccgctgcc ccagcaccag tgcggacgtc
    22261 accggattcg catcctgggc ctcatcaaaa aggatcgtat cccacctgcg tgacagatcc
    22321 ggtgaggaaa gctgatagag cttcagatag gtgtcgtgag tgaccggaaa attgccatcc
    22381 ggattgctca tttcatgcca gaggatctgc acggcacgca gcgcatcctg tggcgacatg
    22441 cccttcatct cgtcgggagg cggcatatgc tgcattccgg gcgccacgtc cgccgaacag
    22501 atgaactgat tcagcgcggt catggcaaca cgggcaagcg tccagtagcg ggtgttcagt
    22561 tttctcgcta catccgtcac tgacagcccc ggcaccagac gatgccggaa atgctttccg
    22621 aaccgcgaat acgccagctg atgggaggtt ttgcactcca cattaaaggg aaaccggcgc
    22681 tccgcctcat cacgaatcgc gcggttatag gccagataca gcatccggct ctcagggcag
    22741 gctgcggcga actgcaccag tgtgctggtt ttgcctgtac cggcaaaggc attcaccacc
    22801 agtttttccc cctgccagtg aatgatggct gactgttcat cggtgggatt cagggacatg
    22861 gcattcctcc gttcacgaaa tgtcagcgca ctctgccttc tcaggagcgt cgctgaccag
    22921 aattaattca ccctgtcaca ccagaaattt cccggcaata aattttcaga aacacaaaaa
    22981 gaattaatcg tcgccatgtg cagagcgccg cagcatcatc ccttcagaca ccgccgtatc
    23041 gttatccgcg cggtgacacc ggacaccatg ccggtaaggc tctttaacaa tcgggtattg
    23101 cggcaacgcc ctgaagccgg gctgaggagg aaacagaatg ctacgttatg agtgacgtgc
    23161 cgcgcctgca gccacaggta acactcatca caccctgccg tatcagtaca ggcgcacaac
    23221 acgaaaatac actgaccgaa tcatcccgtg tatttccggt tgtgcggacc tgcgtccgta
    23281 caacgcaccg ctgcaccagt acctttcagg cgacatcgcg tttttcccct tgttgctgat
    23341 gtcgcctgtt tttccgggac cggaaaccgg caacaggagc catgaatgac agcaacaaca
    23401 gacaccacac cgcataaata ccggattgaa gccaccatcc tgaagcctgg tggtgcaccg
    23461 gtatcctggg tgcgttttca gaacacaccg atgactgagc ctgaatgcgt caggctgttt
    23521 tcaaaagaaa aagaggccgg taaatcgttc agtgtgaaag tgaacgtcac cgcattctca
    23581 tgcagcagag ttcagccgga ataaaaccgg atacaggctc aggaaacaca aaaaatgtgc
    23641 gatacaggac cataatatga caccactgaa accacttctt attgtgccgc tgctggcatt
    23701 ttctgttcca ctttttgctg caccgtcacc ttctcaggtc agtgcaccct catcgtccac
    23761 ggtcagcagc gctccgggag cactgaaatt tattccccgg gacggtaaag ggttatcact
    23821 gaaaaaagcc atgcagcaac tgataccgcc gggatggaca tttgacattg caccggatgt
    23881 caggccgccc cgcacggtgt catggaaggg aaatgaccag tggaattacg ttacggaacg
    23941 catgctgaaa aaatatcagc tccgcgccat caccgacacg gagaagaagc atgtacgtat
    24001 tgagcggaac accctgttca gtccgtctca ggccaccccg ggcaccacca gtctgcagga
    24061 aaaaaccgcg gaaaaatcag caaacatgcc gcaaccggcg catgcacgga ccacctggac
    24121 tcttgcccgc ggaaccctgc tgcgtgccgg tcttgagcag tgggtggccg ccgttccctg
    24181 tcaggccggt ggcacctggc agctggcctg gcagaacccc ggactcaatt acagcatcga
    24241 tgcgccgctg catttcaccg gcgagttccg ccacgcgctc agtgaactct tccgtctcta
    24301 tcaggacgca cagacgccgc tgtatgcagc catcaacagc acgcagtgtg tggtgcacgt
    24361 cagcgacaga ccgggagact gaccatgggc tggtcgctct ccctggtcat ggccctgacc
    24421 gtggcgctga cctggtttca caacagcagt acccaccggg tcaccgtgta cagcgagcag
    24481 gcggagatgc gtgaacgtgc gtgggagatg gtccgcctca tgaacggcat taacgaccgg
    24541 ctgtacaccc gtccggctga gcgcacgggg ggtggcacgc tccccgtcac cgccacgggt
    24601 ttccgcccgg tatacggtac ccgccatatc atcgccggac agcgggtctt tgtctggcag
    24661 gatgaccgcc cggggcttgc aggtgcgctg gcagacatca cccacaaaac cgcgctggca
    24721 ggccgggtga aggggcggcg actgctgaac accacaggtc aggacaccgg tatcagcgtg
    24781 ccgtccgtca ttcctgacag cgcatttgtt tatttcaact agcgggacat cactgcaccg
    24841 ttaaccttcg ctgtcccgca cagtgtgagg agcctgtgga ctgaagtctg catatccggc
    24901 cgcggtcatg tcccgcggat atatcgccat cacgcgcaga cttcagtccc gaagcgcttc
    24961 tgccctccct gctctgctca ctgttttcac gtttcacaca aggatttctg catgaaaaaa
    25021 agacaacaca tgctcctgcc actgactgcc gtcgccctgc tgctgtcagg ctgcggtacg
    25081 gccgaacgta tcagcaacac ggaaaaagac gccaccgcca cactggagaa agcgcgccgg
    25141 cacgcgggcg aactccgtaa atcccggccg gtggtcacgg agaccacgcg acagtggatc
    25201 aacccctccc ctgtcagcgt cagccccgca caccagagcc tgccgggctg ccccatcatc
    25261 atcaacaccc gccgggatat cagcctgcgg cagatagccc agcgcatcac cggaagctgc
    25321 ggtatccccg tgaccatcac cgccgacgtc tggcaggcgc tggccaccac cggccccaca
    25381 cagcagctga caggcgcaat ccccgcaccg gacccgaacg gcatggtgcc gctggccgcc
    25441 gtcggcagtg gcgcacaggt catccccccg ggcggcggtg ccgtcctgcc ggaactcagc
    25501 gccaccgggc tgcggcagct gctggatacc gtcgcctccc ggctcggtat ctcgtaccgt
    25561 tacgacagtg accggcgcgc catcaccttt tactggctgg agacacgcac cttcccggtc
    25621 acctacatgg actctcaggt gacctacaac gcccgggtgg tcagcggcac cgcctcctcc
    25681 tcatccggca gcagtaccgg cagtaacgcc ggcaccctca gcggggaggc cagcaacacc
    25741 cagaccacca cggtcgacat gaagtccacc ctctatgagg acctgaaaaa tgccgtcggt
    25801 gccatgctga cccccgggac cggccggatg gtgcttgcca ccggctttct caccgtcacc
    25861 gacacccctc gggtcctcga caccgtcagc cgttttgtgg aggcacgtaa ccgcgagatg
    25921 cgacgtcagg tggtgctgaa cgtggaagtc ctcagcgtca gaaaaacccg ccgtgaccag
    25981 gccggcattg actggaatgc cgtcttcagc gacggacgtc tcggcctcac gctgggaggg
    26041 gcattcagca gtgccgccga tgacatcatc accagtggcg tctccattgt ggacggtaag
    26101 ctcaccggct cgaaggcctt cctgaaggcg ctctccagtc agggcaccgt cagcgtggtc
    26161 acacagaact ccgccgtcac caaaaacatg acgccggtgc ccatgcaggt ggccagccag
    26221 cagggctata tcgaaggtgt caccaccgac accacggcca acgtcgggac cagcacctcc
    26281 ctgaacgccg ccaccatcac caccggcttc aacatgaccc tgctgccgtt cattcagccg
    26341 gatgcgcgca gcctgcagct gctcttcagc atgaacctct ccgacaaacc cacctttgag
    26401 gtgttcgaga gcggcggcag ccgggcacag gtgcccacgg tagacctgaa aaccatcaac
    26461 cagaccatcg acctgaaatc aggtcagacc gtcattctca gcggattccg gcagtccggc
    26521 agcaaatccg tgcgccaggg cgtcggcagc ccggcattct tcgggctggg tggcggcact
    26581 gacagcggtg acgatgacac cattctggtt gtgctgatca caccgcacgt aatctgacgg
    26641 aggttccctt gaacgggtca atgcagaaaa aaattcgtcc ggtttcactg atatcgggta
    26701 aacaggcatt ccttgcgggc cttgactgga agagcatacc tcccggttac cggaatgccc
    26761 ggcggtttgc ccggtcacag ggcgcagaac tgtatctttc ctgccacaac cacgacggtg
    26821 aaaacggtga tgaaatgatg attgccctgc tcagtcgtca ggatgcagat atcccccgta
    26881 acattcgcca gtgcctgtca ctggcaatgt taatacgccc gctactcaaa tcaggtgggt
    26941 atgccatttt catcatcgag aacgacgacg ctgaactcac ctgtgcgttt gtatcggtga
    27001 ttaatggcat tctggttaac gacgttaccg gtaccccgac tgaaatcacg gaagcccgca
    27061 acacgttcct gatgctcaat gcggaaccgg aaaacggctg ggtacgctac gaacctgacg
    27121 gtttttcacc agacagtcgg tgtatcccgc taccgctgtc aaacctgatc agcaccagaa
    27181 agcatccggc agaagcgcgt ctgctccccg tgtccagagg cccacagtta atgacagcct
    27241 tactcatcat cacgctgctt ggcgcatcgt ggtacggctg gcaacgttat gaaatctggc
    27301 aggcggaaaa agcggaacgg gaagaaagcg cccggaaagc agctgaagca cgtataacgc
    27361 caccatggac gaggcagcct gagagcggag aatttatccg gcaatgcagc acaacatgga
    27421 accagacgcc actgtctgct gccggatggc gttataccct ggctgaatgt tctactgacg
    27481 gtaacagcgg taatctgcgg tcttcataca ccaatacagc aggcacgaca gtgacagcct
    27541 tcatccggcg catcactgaa ctcacagaca cccggccgtt tattgttatg cctgaaggga
    27601 actctggcgg cgttgcactg ccggtcacat tccggctccc ggataatcct gtcccggttg
    27661 attcccttcc ggaaacccga gacctgcagg aacgactgac cacgctcgca cagtcagttc
    27721 aactgcagat tgactggcag gaagtcaata acagcttcac tgatgaaaac ggaaacatca
    27781 tccagccacc gtggaaagag tatgacctgc agagatacag acgctactcc cggctaacca
    27841 gctggcagag cgcttcagcg agccatctgt ccgttttctc tctgtcacaa gacaactgga
    27901 aaatggtcgt ttccgttatc agttcacagg taaatattat gcacgataaa acaaaagcgc
    27961 tgctgattgc agccctgctg ttaccggcaa tggcagtggc cggcaacgaa aatgacgcgt
    28021 cacagaacaa acaactgcag attgagcaga ttcaggaaga aaccgccctg tatgaagcga
    28081 tgctggcaag agaaaaaatt cgtcatgaat tgcggacact ggaacagggt atctccccgg
    28141 tttccggttc tgtcacggga catcagccgg aaaatgcggc tggcacctcc tccggttcac
    28201 aggcattttc atccccccgt ctgaaagaaa tctttggtat tcagccacag ctttcagcac
    28261 gcgtcatact ccccggagga actgaaactg atgtcagggc cggacaggca ttacccggta
    28321 cactgttcat ggtggaacaa atcacaccgg acagtgtgat tctggaatac aacggcaaca
    28381 ggcagacgct acgcccgtga ggtgccatca tgccagagac tatcgaactg cctcctgaag
    28441 atattcgtga tttcgtcgca attcagcatg acgctgacgg aaaaatcagg gtgaaagtcg
    28501 tcgaggcgaa gaagcacatc acctctgtac aacgttacct gacgctctta cgctcccgct
    28561 atccgggaaa aatcagcaaa ttcgatttta ccactcttga cgttatcgca accctgaaca
    28621 gcaataatac ggaacgtgca ccgggcgttt tatccgacgg agggatcaac acagtacaaa
    28681 gaaaagttct tgattacatc agaaaagccg ctcaactgga tgcctcagat ctgcatatca
    28741 caccaggacg
//
LOCUS       DAMZFT010000056        28432 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.056, whole genome shotgun sequence.
ACCESSION   DAMZFT010000056 DAMZFT010000000
VERSION     DAMZFT010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 28432)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28432)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28432
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.056"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(<1..149)
                     /locus_tag="PN977_002525"
     CDS             complement(<1..149)
                     /locus_tag="PN977_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418120.2"
                     /note="involved in the transport or adenine; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HDI6042545.1"
                     /translation="MNNDNTDYVSNESGTLSRLFKLPQHGTTVRTELIAGMTTFLTMV
                     YIVFV"
     gene            324..2090
                     /gene="adeD"
                     /locus_tag="PN977_002526"
     CDS             324..2090
                     /gene="adeD"
                     /locus_tag="PN977_002526"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HDI6042546.1"
                     /translation="MNNSINHKFHHISRAEYQELLAVSRGDAVADYIIDNVSILDLIN
                     GGEISGPIVIKGRYIAGVGAEYTDAPALQRIDARGATAVPGFIDAHLHIESSMMTPVT
                     FETATLPRGLTTVICDPHEIVNVMGEAGFAWFARCAEQARQNQYLQVSSCVPALEGCD
                     VNGASFTLEQMLAWRDHPQVTGLAEMMDYPGVISGQNALLDKLDAFRHLTLDGHCPGL
                     GGKELNAYIAAGIENCHESYQLEEGRRKLQLGMSLMIREGSAARNLNALATLINEFNS
                     PQCMLCTDDRNPWEIAHEGHIDALIRRLIEQHNVPLHVAYRVASWSTARHFGLNHLGL
                     LAPGKQADIVLLSDARKVTVQQVLVKGEPIDAQTLQAEESARLAQSAPPYGNTIDRQP
                     VSASDFALQFTPGKRYRVIEAIHNELITHSRSSVYSENGFDRDDVCFIAVLERYGQRL
                     APACGLLGGFGLNEGALAATVSHDSHNIVVIGRSAEEMALAVNQVIQDGGGLCVVRNG
                     QVQSHLPLPIAGLMSTDTAQSLAEQIDALKAAARECGPLPDEPFIQMAFLSLPVIPAL
                     KLTSQGLFDGEKFAFTTLEVTE"
     gene            complement(2137..3528)
                     /gene="uhpT"
                     /locus_tag="PN977_002527"
     CDS             complement(2137..3528)
                     /gene="uhpT"
                     /locus_tag="PN977_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexose-6-phosphate:phosphate antiporter"
                     /protein_id="HDI6042547.1"
                     /translation="MLAFLNQVRKPTLDLPLEVRRKMWFKPFMQSYLVVFIGYLTMYL
                     IRKNFNIAQNDMISTYGLSMTQLGMIGLGFSITYGVGKTLVSYYADGKNTKQFLPFML
                     ILSAICMLGFSASMGSGSVSLFLMIAFYALSGFFQSTGGSCSYSTITKWTPRRKRGTF
                     LGFWNISHNLGGAGAAGVALFGANYLFDGHVIGMFIFPSIIALIVGFIGLRYGSDSPE
                     SYGLGKAEELFGEEISEEDKETESTDMTKWQIFVEYVLKNKVIWLLCFANIFLYVVRI
                     GIDQWSTVYAFQELKLSKAVAIQGFTLFEAGALVGTLLWGWLSDLANGRRGLVACIAL
                     ALIIATLGVYQHASNQYIYLASLFALGFLVFGPQLLIGVAAVGFVPKKAIGAADGIKG
                     TFAYLIGDSFAKLGLGMIADGTPVFGLTGWAGTFAALDIAAIGCICLMAIVAVMEERK
                     IRREKKIQQLTVA"
     gene            complement(3666..4985)
                     /gene="uhpC"
                     /locus_tag="PN977_002528"
     CDS             complement(3666..4985)
                     /gene="uhpC"
                     /locus_tag="PN977_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709527.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter family glucose-6-phosphate
                     receptor UhpC"
                     /protein_id="HDI6042548.1"
                     /translation="MLPFLKAPADAPLISDKQEIDARYRYWRRHILLTIWLGYALFYF
                     TRKSFNAAVPEILANGVLSRSDIGLLATLFYITYGVSKFVSGIVSDRSNARYFMGIGL
                     IATGIINILFGFSTSLWAFAVLWVLNAFFQGWGSPVCARLLTAWYSRTERGGWWALWN
                     TAHNVGGALIPIVMAASALHYGWRAGMMIAGCMAIVVGIFLCWRLRDRPQALGLPAVG
                     EWRHDALEIAQQQEGAGLTRKEILTKYVLLNPYIWLLSFCYVLVYVVRAAINDWGNLY
                     MSETLGVDLVTANTAVTMFELGGFIGALVAGWGSDKLFNGNRGPMNLIFAAGILLSVG
                     SLWLMPFASYVMQATCFFTIGFFVFGPQMLIGMAAAECSHKEAAGAATGFVGLFAYLG
                     ASLAGWPLAKVLDTWHWSGFFVVISIAAGISALLLLPFLNAQTPREA"
     gene            complement(4995..6497)
                     /gene="uhpB"
                     /locus_tag="PN977_002529"
     CDS             complement(4995..6497)
                     /gene="uhpB"
                     /locus_tag="PN977_002529"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312632.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction histidine-protein
                     kinase/phosphatase UhpB"
                     /protein_id="HDI6042549.1"
                     /translation="MKTFFSRLITVIACFFIFSAAWFCLWSISLHLVERPDMAVLLFP
                     FGLRLGLMLQCPRGYWPVLLGAEWLLIYWLTQAVGLTHFSLLMIGSLLTLLPVALISR
                     YRHQRDWRTLLLQGAALTAAALLQSLPWLWHGKESWNALLLTLTGGLTLAPICLVFWH
                     YLANNIWLPLGPSLVSQPINWRGRHLVWYLLLFVISLWLQLGLPDELSRFTPFCLALP
                     IIALAWHYGWQGALIATLMNAIALIASQTWRDHSVDLLLSLLVQSLTGLLLGAGIQRL
                     RELNQSLQKELARNQHLAERLLETEESVRRDVARELHDDIGQTITAIRTQAGIVQRLA
                     ADNASVKQSGQLIEHLSLGVYDAVRRLLGRLRPRQLDDLTLEQAIRSLMREMELEGRG
                     IVSHLEWRIDESALSENQRVTLFRVCQEGLNNIVKHADASAVTLQGWQQDERLMLVIE
                     DDGSGLPPDSGQHGFGLTGMRERVTALGGTLTISCLHGTRVSVSLPQRYV"
     gene            complement(6497..7087)
                     /gene="uhpA"
                     /locus_tag="PN977_002530"
     CDS             complement(6497..7087)
                     /gene="uhpA"
                     /locus_tag="PN977_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator UhpA"
                     /protein_id="HDI6042550.1"
                     /translation="MITVALIDDHLIVRSGFAQLLGLEPDLQVVAEFGSGREALAGLP
                     GRGVQVCICDISMPDISGLELLSQLPKGMATIMLSVHDSPALVEQALNAGARGFLSKR
                     CSPDELIAAVHTVATGGCYLTPDIAIKLASGRQDPLTKRERQVAEKLAQGMAVKEIAA
                     ELGLSPKTVHVHRANLMEKLGVSNDVELARRMFDGW"
     gene            complement(7249..8687)
                     /locus_tag="PN977_002531"
                     /pseudo
     CDS             complement(7249..8687)
                     /locus_tag="PN977_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842408.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(8966..9448)
                     /locus_tag="PN977_002532"
     CDS             complement(8966..9448)
                     /locus_tag="PN977_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6042551.1"
                     /translation="MVAQVCFNDDDCARVRELLKQSNGNITDDKIDEIIACLASPQGI
                     TCFYYSSAQGYQSRLQHTSQDVRELLSKSFDSFISFTTDLKKSGNSFFGSGKYLNEMD
                     FTINGFSQKMYLMAVNNVGNEVKLQCSRYFTTGRVDEQNSKNDKVAFTFFGAATTADL
                     "
     gene            complement(10524..10814)
                     /gene="ilvN"
                     /locus_tag="PN977_002533"
     CDS             complement(10524..10814)
                     /gene="ilvN"
                     /locus_tag="PN977_002533"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HDI6042552.1"
                     /translation="MQNTTHDNVILELTVRNHPGVMTHVCGLFARRAFNVEGILCLPI
                     QDSDKSHIWLLVNDDQRLEQMISQIDKLEDVVKVQRNQSDPTMFNKIAVFFQ"
     gene            complement(10818..12506)
                     /gene="ilvB"
                     /locus_tag="PN977_002534"
     CDS             complement(10818..12506)
                     /gene="ilvB"
                     /locus_tag="PN977_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HDI6042553.1"
                     /translation="MASSGTTSTRKRFTGAEFIVHFLEQQGIKIVTGIPGGSILPVYD
                     ALSQSTQIRHILARHEQGAGFIAQGMARTDGKPAVCMACSGPGATNLVTAIADARLDS
                     IPLICITGQVPASMIGTDAFQEVDTYGISIPITKHNYLVRHIEELPQVMSDAFRIAQS
                     GRPGPVWIDIPKDVQTAVFEIEAQAAMAEKAAAPAFSEESIRDAAAMINAAKRPVLYL
                     GGGVINAPARVRELAEKAQLPTTMTLMALGMLPKAHPLSLGMLGMHGVRSTNYILQEA
                     DLLIVLGARFDDRAIGKTEQFCPNAKIIHVDIDRAELGKIKQPHVAIQADVDDVLAQL
                     IPQVEAQPRAEWHQLVADLQREFPCPIPKACDPLTHYGLINAVAACVDDNAIITTDVG
                     QHQMWTAQAYPLNRPRQWLTSGGLGTMGFGLPAAIGAALANPDRKVLCFSGDGSLMMN
                     IQEMATASENQLDVKIILMNNEALGLVHQQQSLFYKQGVFAATYPGKINFMQIAAGFG
                     LETCDLNNETDPQAALQEIINRPGPALIHVRIDAEEKVYPMVPPGAANTEMVGE"
     gene            complement(12612..12710)
                     /gene="ivbL"
                     /locus_tag="PN977_002535"
     CDS             complement(12612..12710)
                     /gene="ivbL"
                     /locus_tag="PN977_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ilvB operon leader peptide IvbL"
                     /protein_id="HDI6042554.1"
                     /translation="MTTSMFNAKLLPTAPSAAVVVVRVVVVVGNAP"
     gene            13275..13364
                     /gene="tisB"
                     /locus_tag="PN977_002536"
     CDS             13275..13364
                     /gene="tisB"
                     /locus_tag="PN977_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin TisB"
                     /protein_id="HDI6042555.1"
                     /translation="MSLVDIAILILKLIVAALQLLDAVLKYLK"
     gene            13644..14828
                     /gene="emrD"
                     /locus_tag="PN977_002537"
     CDS             13644..14828
                     /gene="emrD"
                     /locus_tag="PN977_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EmrD"
                     /protein_id="HDI6042556.1"
                     /translation="MKRHRNVNLLLMLVLLVAVGQMAQTIYIPAIADMARDLNVREGA
                     VQSVMGAYLLTYGVSQLFYGPISDRVGRRPVILVGMSIFMLATLVAVTTSSLTVLIAA
                     SAMQGMGTGVGGVMARTLPRDLYERTQLRHANSLLNMGILVSPLLAPLIGGLLDTMWN
                     WRACYLFLLVLCAGVTFSMARWMPETRPVDAPRTRLLTSYKTLFGNSGFNCYLLMLIG
                     GLAGIAAFEACSGVLMGAVLGLSSMTVSILFILPIPAAFFGAWFAGRPNKRFSTLMWQ
                     SVICCLLAGLLMWIPDWFGVMNVWTLLVPAALFFFGAGMLFPLATSGAMEPFPFLAGT
                     AGALVGGLQNIGSGVLASLSAMLPQTGQGSLGLLMTLMGLLIVLCWLPLATRMSHQGQ
                     PV"
     gene            complement(14836..15333)
                     /locus_tag="PN977_002538"
     CDS             complement(14836..15333)
                     /locus_tag="PN977_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HDI6042557.1"
                     /translation="MTGSQVIDAEEDRHKLVVEYKDALQPADFYHNFKQRGIRSVQLI
                     PHLEFDDRGDLTAASVTAELWGKFLIALFECWVRADISRISIELFDATLQKWCGSENP
                     QPRRGCQACDWHRLCPHAREETPDNVLCAGYQAFYSYSAPHMRVMRDLIKQHRSPMEL
                     MTMLR"
     gene            complement(15330..15692)
                     /locus_tag="PN977_002539"
     CDS             complement(15330..15692)
                     /locus_tag="PN977_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF202 domain-containing protein"
                     /protein_id="HDI6042558.1"
                     /translation="MPDSRKARRIADPGLQPERTSLAWFRTMLGYGALMALAIKHNWH
                     QAGMLFWISIGILAIVALILWHYTRNRNLMDVTNSDFSQFHVVRDKFLISLAVLSLAI
                     LFAVTHIHQLIVFIERVA"
     gene            complement(15682..16029)
                     /locus_tag="PN977_002540"
     CDS             complement(15682..16029)
                     /locus_tag="PN977_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidH family protein"
                     /protein_id="HDI6042559.1"
                     /translation="MKISRLGEAPDYRFSLANERTFLAWIRTALGFLAAGVGLDQLAP
                     DFATPVIRELLALLLCLFSGGLAMYGYLRWLRNEKAMRLKEDLPYTNSLLIISLILMV
                     VAVIVMGLVLYAG"
     gene            complement(16089..17582)
                     /locus_tag="PN977_002541"
     CDS             complement(16089..17582)
                     /locus_tag="PN977_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HDI6042560.1"
                     /translation="MKRPNFLFIMTDTQATNMVGCYSGKPLNTQNIDSLAAESIRFNS
                     AYTCSPVCTPARAGLFTGIYANQSGPWTNNVAPGKNISTMGRYFKDAGYHTCYIGKWH
                     LDGHDYFGTGECPPEWDADYWFDGANYLSELTEKEISLWRNGLNSVEDLQANHIDETF
                     TWAHRISNRAVDFLQQPARAEEPFLMVVSYDEPHHPFTCPVEYLEKYADFYYELGEKS
                     QDDLANKPEHHRLWAQAMPSPVGDDGLYHHPLYFACNDFVDDQIGRVINALTPEQREN
                     TWVIYTSDHGEMMGAHKLISKGAAMYDDITRIPLIIRSPQGERRQVDTPVSHIDLLPT
                     MMALADIEKPEILPGENILAVKEPRGVMVEFNRYEIEHDSFGGFIPVRCWVTDDFKLV
                     LNLFTSDELYDRRNDLNEMHNLIDDIRFADVRRKMHDALLDYMDKIRDPFRSYQWSLR
                     PWRKDARPRWMGAFRPRPQDGYSPVVRDYDTGLPTQGVKVEEKKQKF"
     gene            complement(17579..19294)
                     /locus_tag="PN977_002542"
     CDS             complement(17579..19294)
                     /locus_tag="PN977_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="solute:sodium symporter family transporter"
                     /protein_id="HDI6042561.1"
                     /translation="MNSLQILSFVGFTLLVAIITWWKVRKTDTGSQQGYFLAGRSLKA
                     PVIAASLMLTNLSTEQLVGLSGQAYKSGMSVMGWEVTSAVTLIFLALIFLPRYLKRGI
                     ATIPDFLEERYDKTTRIIIDFCFLIATGVCFLPIVLYSGALALNSLFHVGESLQISHG
                     AAIWLLVILLGLAGILYAVIGGLRAMAVADSINGIGLVIGGLMVPIFGLIAMGKGSFM
                     QGIEQLTTVHAEKLNSVGGPTDPLPIGAAFTGLILVNTFYWCTNQGIVQRTLASKSLA
                     EGQKGALLTAVLKMLDPLVLVLPGLIAFHLYQDLPKADMAYPTLVNNVLPVPLVGFFG
                     AVLFGAVISTFNGFLNSASTLFSMGIYRRIINQNAEPQQLVTVGRKFGFFIAIVSVLV
                     APWIANAPQGLYSWMKQLNGIYNVPLVTIIIMGFFFPRIPALAAKVAMGIGIISYITI
                     NYLVKFDFHFLYVLACTFCINVVVMLVIGFIKPRATPFTFKDAFAVDMKPWKNVKIAS
                     IGILFAMIGVYAGLAEFGGYGTRWLAMISYFIAAVVIVYLIFDSWRHRHDPAVTFTPD
                     AKDSL"
     gene            19461..20354
                     /locus_tag="PN977_002543"
     CDS             19461..20354
                     /locus_tag="PN977_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418136.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HDI6042562.1"
                     /translation="MNGKLQSSDVKNETPYNIPLLINENVISSGISLISLWHTYADEH
                     YRVIWPRDKKKPLIANSWVAVYTVQGCGKIILKNGEQITLHGNCIIFLKPMDIHSYHC
                     EGLVWEQYWMEFTPTSMMDIPVGQQSVIYNGEIYNQELTEVAELITSPEAIKNNLAVA
                     FLTKIIYQWICLMYADGKKDPQRRQIENLIATLHASLQQRWSVADMAATIPCSEAWLR
                     RLFLRYTGKTPKEYYLDARLDLALSLLKQQGNSVGEVADTLNFFDSFHFSKAFKNKFG
                     YAPSAVLKNTDQHPTDASPHN"
     gene            complement(20351..21673)
                     /locus_tag="PN977_002544"
     CDS             complement(20351..21673)
                     /locus_tag="PN977_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-alpha-glucosidase"
                     /protein_id="HDI6042563.1"
                     /translation="MTKFSVVVAGGGSTFTPGIVLMLLANQDRFPLRALKFYDNDGAR
                     QEVIAEACKVILKEKAPDIAFSYTNDPEVAFSDVDFVMAHIRVGKYPMRELDEKIPLR
                     HGVVGQETCGPGGIAYGMRSIGGVLELVDYMEKYSPNAWMLNYSNPAAIVAEATRRLR
                     PNAKILNICDMPIGIESRMAQIVGLQDRKQMRVRYYGLNHFGWWTSIEDLQGNDLMPQ
                     LRQYVSKHGYVPPQQDTHTEASWNDTYAKARDVQALDPDTLPNTYLKYYLFPDYVVQH
                     SNPEHTRANEVMEHREKQVFDACRAITAAGNSAAGKLEIDEHASYIVDLAAAIAFNTQ
                     ERMLLIVPNNGAIHNFDDEAMVEIPCLVGHNGPEPLVVGEIPQFQKGLMSQQVAVEKL
                     VVDAWEQRSYQHLWQAITLSKTVPSAAVAKAILDELLEANKAYWPELR"
     gene            complement(21673..23289)
                     /locus_tag="PN977_002545"
     CDS             complement(21673..23289)
                     /locus_tag="PN977_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDI6042564.1"
                     /translation="MLSQIQRFGGAMFTPVLLFPFAGIVVGLAILLQNPMFVGESLTD
                     PNSLFAQIVHIIEEGGWTVFRNMPLIFAVGLPIGLAKQAQGRACLAVMVSFLTWNYFI
                     NAMGMTWGSYFGVDFTQDAVAGSGLTMMAGIKTLDTSIIGAIIISGIVTALHNRLFDK
                     KLPVFLGIFQGTSYVVIIAFLVMIPCAWLTLLGWPKVQMGIESLQAFLRSAGALGVWV
                     YTFLERILIPTGLHHFIYGPFIFGPAAVEGGIQMYWAQHLQEFSLSAEPLKSLFPEGG
                     FALHGNSKIFGAVGISLAMYFTAAPENRVKVAGLLIPATLTAMLVGITEPLEFTFLFI
                     SPLLFAVHAVLAASMSTVMYLFGVVGNMGGGLIDQVLPQNWIPMFSNHADMILTQIAI
                     GLCFSLLYFVVFRTLILQFNMCTPGREDAEVKLYSKAEYKASRGQTTAAEPKKELDQA
                     AGILQALGGVGNISSINNCATRLRIALHDMSQTLDDEVFKKLGAHGVFRSGDAIQVII
                     GLHVSQLREQLDSLINSHQSAENVAITEAV"
     gene            23585..24301
                     /locus_tag="PN977_002546"
     CDS             23585..24301
                     /locus_tag="PN977_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDI6042565.1"
                     /translation="MIYKSIAERLRIRLNSADFTLNSLLPGEKKLAEEFAVSRMTIRK
                     AIDLLVAWGLVVRRHGSGTYLVRKDVLHQTASLTGLVEVLKRQGKTVTSQVLIFEIMP
                     APPAIASQLRIQINEQIYFSRRVRFVEGKPLMLEDSYMPVKLFRNLSLQHLEGSKFEY
                     IEQECGILIGGNYESLTPVLADRLLARQMKVAEHTPLLRITSLSYSESGEFLNYSVMF
                     RNASEYQVEYHLRRLHPEKS"
     gene            complement(24298..25959)
                     /locus_tag="PN977_002547"
     CDS             complement(24298..25959)
                     /locus_tag="PN977_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative transporter"
                     /protein_id="HDI6042566.1"
                     /translation="MSDIALTVSILALVAVVGLFIGNVKFRGIGLGIGGVLFGGIIVG
                     HFVSQAGMTLSSDMLHVIQEFGLILFVYTIGIQVGPGFFASLRVSGLRLNLFAVLIVI
                     IGGLVTAILHKLFDIPLPVVLGIFSGAVTNTPALGAGQQILRDLGTPMEMVDQMGMSY
                     AMAYPFGICGILFTMWMLRVIFRVNVETEAQQHESSRTNGGALIRTINIRVENPNLHD
                     LAIKDVPILNGDKIICSRLKREETLKVPSPDTIIQLGDLLHLVGQPADLHNAQLVIGQ
                     EVDTSLSTKGTDLRVERVVVTNENVLGKRIRDLHFKERYDVVISRLNRAGVELVASGD
                     ISLQFGDILNLVGRPSAIDAVANVLGNAQQKLQQVQMLPVFIGIGLGVLLGSIPVFVP
                     GFPAALKLGLAGGPLIMALILGRIGSIGKLYWFMPPSANLALRELGIVLFLSVVGLKS
                     GGDFVNTLVNGEGLSWIGYGALITAVPLITVGILARMLAKMNYLTMCGMLAGSMTDPP
                     ALAFANNLHPTSGAAALSYATVYPLVMFLRIITPQLLAVLFWSIG"
     gene            complement(26155..26583)
                     /gene="ibpB"
                     /locus_tag="PN977_002548"
     CDS             complement(26155..26583)
                     /gene="ibpB"
                     /locus_tag="PN977_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock chaperone IbpB"
                     /protein_id="HDI6042567.1"
                     /translation="MRNFDLSPLMRQWIGFDKLANALQNAGESQSFPPYNIEKSDDNH
                     YRITLALAGFRQEDLEIQLEGTRLSVKGTPEQPKEEKKWLHQGLMNQPFSLSFTLAEN
                     MEVSGATFVNGLLHIDLIRNEPEPIAAQRIAISERPALNS"
     gene            complement(26695..27108)
                     /gene="ibpA"
                     /locus_tag="PN977_002549"
     CDS             complement(26695..27108)
                     /gene="ibpA"
                     /locus_tag="PN977_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006687722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock chaperone IbpA"
                     /protein_id="HDI6042568.1"
                     /translation="MRNFDLSPLYRSAIGFDRLFNHLENNQSQSNGGYPPYNVELVDE
                     NHYRIAIAVAGFAESELEITAQDNLLVVKGAHADEQKERTYLYQGIAERNFERKFQLA
                     ENIHVRGANLVNGLLYIDLERVIPEAKKPRRIEIN"
     misc_feature    complement(27102..27174)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01832"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ROSE (Repression Of Heat Shock gene Expression)
                     occurs in the 5'-region of heat shock genes and acts as an
                     RNA thermometer to modulate expression; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF01832"
     gene            27414..27746
                     /locus_tag="PN977_002550"
     CDS             27414..27746
                     /locus_tag="PN977_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HDI6042569.1"
                     /translation="MIRNVLLAFMICSGMTLLGGCSSVMSHTGGKEGTYPGTRASATM
                     IGDDETNWGTKSLAILDMPFTAVLDTILLPWDVFRKDSSVRSRVEKSEANAQATNAVI
                     PPARMPDN"
     gene            complement(27748..>28432)
                     /locus_tag="PN977_002551"
     CDS             complement(27748..>28432)
                     /locus_tag="PN977_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312656.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3748 domain-containing protein"
                     /protein_id="HDI6042570.1"
                     /translation="EYSGSHWCVLVSKTTPTPQPGSDEINRAYEEGWVGNHALAFIGD
                     TLSPKGEKVPELFIVELPQDEAGWKAAGDAPLSGTETTLPAPPRGVVQRRLTFTHHRA
                     YPGLVNVPRHWVRCNPQGTQIAFLMRDDNGIVQLWLISPQGGEPRQLTHNKTDIQSAF
                     NWHPSGEWLGFVLDNRIACAHAQSGEVEYLTENHANPPSADAVVFSPDGQWLAWMEGG
                     QLWITETDR"
BASE COUNT         7269 a         7445 c         6904 g         6814 t
ORIGIN      
        1 ttcacaaaaa cgatgtacac catggttaaa aaagtggtca tccccgcaat caattctgtg
       61 cggacggtgg tcccatgctg aggtagttta aataatcgcg aaagcgtccc tgattcatta
      121 ctcacgtaat cggtattgtc attattcatt tttttgtcca ttggaggaga tttaatccct
      181 tgcttttgta aaacaaatga caacaacggt tcagtgataa ttatcacatt tcaattgcac
      241 attaatggat attctttaat aatctcgcga cgtttcttta tgataaataa taatcaaatt
      301 gataaaatca aaatgagaaa aatatgaata attctattaa ccataaattt catcacatta
      361 gccgggctga ataccaggaa ttgttagccg tttcccgtgg cgacgctgtt gccgattata
      421 ttattgataa tgtctctatt ctcgacctga tcaatggcgg agaaatttcc ggcccaattg
      481 tgattaaagg acgttacatt gctggtgttg gcgcagaata cactgatgct ccggctttgc
      541 agcggattga tgcccgcggc gcaacggcgg tgccagggtt tattgatgct cacctgcata
      601 ttgaatccag catgatgacg ccggtcacct ttgaaaccgc taccctgccg cgtggcctga
      661 cgaccgttat ttgcgaccct catgaaatcg tcaacgtgat gggcgaagcc ggattcgcct
      721 ggtttgcccg ctgtgccgaa caggcaagac aaaaccagta cttacaggtc agctcttgcg
      781 tacccgccct ggaaggctgc gatgttaacg gtgccagttt tacccttgaa cagatgctcg
      841 cctggcggga ccatccgcag gttaccggcc ttgcagaaat gatggactac cctggcgtaa
      901 ttagcgggca gaatgcgctg ctcgataaac tggatgcatt tcgccacctg acgctggacg
      961 gtcactgccc gggtttgggt ggtaaagaac ttaacgccta tattgctgcg ggtattgaaa
     1021 actgccacga aagttatcag ctggaagaag gacgccggaa attacaactc ggcatgtcgt
     1081 tgatgatccg cgaagggtcc gctgcccgca atctcaacgc actggcaacg ttgatcaacg
     1141 aatttaacag cccgcaatgc atgctctgta ctgatgaccg taacccgtgg gagatcgccc
     1201 atgaaggaca catcgatgcc ttaattcgcc gcctgatcga acaacacaat gtgccgctgc
     1261 atgtggcata tcgcgtcgcc agctggtcga cggcgcgcca ctttggtctg aatcacctcg
     1321 gcttactggc acccggtaag caggccgata tcgtcctgtt gagcgatgcg cgtaaggtca
     1381 cggtgcagca ggtactggtg aaaggcgagc cgatcgatgc acaaacctta caggcggaag
     1441 agtcggcgag actggcacaa tccgccccgc catatggcaa taccattgat cgccagccag
     1501 tttccgccag tgactttgcc ctgcaattta cccccggaaa acgctatcgc gttattgagg
     1561 ccatccataa cgaattgatt acccactccc gctccagcgt ctacagcgaa aatggttttg
     1621 atcgcgatga tgtgtgcttt attgccgtac ttgagcgtta cgggcaacgg ctggctccgg
     1681 cctgtggttt gctcggcggc tttggcctga atgaaggtgc gctggcggcg acggtcagcc
     1741 atgacagcca taatattgtg gtgatcggtc gtagcgcaga agagatggcg ctggcggtca
     1801 atcaggtgat tcaggatggc ggcgggctgt gcgtggtccg taacggtcag gtacaaagtc
     1861 atctaccgtt gcccattgcc gggctaatga gcaccgacac ggcgcagtca ctggcggagc
     1921 aaattgacgc cttgaaagcc gccgcccgtg aatgcggtcc gttacccgat gagccgttta
     1981 ttcagatggc gtttctttct ctaccagtga tccccgcgct gaaactaacc agtcaggggc
     2041 tgtttgatgg cgagaagttt gccttcacta cgctggaagt cacggaataa taaaaaaaag
     2101 cccggcgtca tgccgggcaa aagtcaccag ttacgtttat gccactgtca actgctgaat
     2161 ttttttctcg cggcggattt tgcgttcttc catcaccgcc actatcgcca tcaggcagat
     2221 acaaccaatc gcggcgatat ccagcgcggc gaaggtgcct gcccagccgg taaggccaaa
     2281 taccggcgtc ccatcagcaa tcattcccag acctaacttg gcaaagctgt caccaatcag
     2341 gtaagcgaag gtgcctttaa taccatcggc agcgccaatc gcttttttag gtacaaagcc
     2401 aacagcagct acaccaatca acaattgcgg gccaaagacc aggaaaccca acgcaaagag
     2461 agaagccagg tagatatatt ggttgctggc gtgttgatac acaccgagcg tggcgataat
     2521 cagcgccagc gcgatacagg ccaccaggcc acggcgaccg ttcgccaggt cagagagcca
     2581 gccccacagc agcgtaccga ccagcgcacc agcttcaaac agcgtaaagc cctgaatcgc
     2641 caccgcttta gagagtttca gttcctggaa cgcatacacg gttgaccact ggtcgatacc
     2701 aatacgcacc acatagagga aaatgttggc aaagcacagc agccagatca ctttgttttt
     2761 cagcacatac tcaacaaaga tctgccactt ggtcatatcg gtagattctg tctctttatc
     2821 ctcttcactg atctcctcgc cgaacagctc ttcagctttg ccgaggccat aagattccgg
     2881 ggagtcgctg ccgtaacgca ggccgataaa accaacaatc agcgcgataa tcgacgggaa
     2941 gataaacatg ccgataacat gaccatcgaa caggtaattt gcgccgaaca gcgccacacc
     3001 tgctgcgcct gcaccgccaa ggttgtgaga aatgttccag aaaccgagga aggtcccgcg
     3061 cttacgacgc ggcgtccatt tggtgatggt ggagtaactg cacgaaccgc cggtgctctg
     3121 gaaaaagccg cttaaggcgt agaaggcaat catcaggaac aggctaaccg agccgctgcc
     3181 catactggcg ctgaagccca gcatacaaat ggcagacagg atcagcatga acggcaggaa
     3241 ttgtttggtg tttttgccgt cggcgtagta ggaaaccagc gttttaccca cgccataagt
     3301 gatggagaaa cccaggccga tcatccccag ctgcgtcatg ctcaacccgt aggtcgaaat
     3361 catatcgttc tgcgcgatgt taaagttctt gcgaatcagg tacatcgtca ggtagccgat
     3421 aaagaccacc aggtaggatt gcatgaacgg tttgaaccac attttgcgcc gcacttcgag
     3481 cggaaggtcc agggtcggct tgcgaacctg gtttaagaaa gccagcatgg gttactcctg
     3541 aaatgaatac ctgcgtgagg catgcattgt aaaaatcagt agcaaatcag gcctgagata
     3601 gcgtccaggc aaaacctgga aaatttctta gttttgccgg atatgaagtg aaaaggtgag
     3661 atgcatcacg cttcgcgcgg tgtctgggcg ttcaaaaagg gcagtaacag cagtgcggaa
     3721 atcccggcgg cgatagagat aaccacaaaa aatccgctcc agtgccaggt atcgagcact
     3781 ttcgccagcg gccaaccagc aagcgacgcc cccagataag caaacaagcc gacaaacccc
     3841 gtcgccgccc ctgccgcctc tttgtgggaa cattctgccg ccgccatgcc gattaacatc
     3901 tgcgggccaa agacaaaaaa accaatggtg aagaagcagg ttgcctgcat cacgtagctg
     3961 gcaaatggca tcagccacag cgagccgact gaaagcaaaa ttccggcagc gaaaatcaaa
     4021 ttcatcggcc cgcggttgcc gttaaagagt ttgtccgagc cccaacccgc taccagcgca
     4081 ccgataaatc cgcccagttc aaacatcgtc actgccgtat tcgccgtgac cagatcgacg
     4141 cccagcgtct cggacatata caaattgccc cagtcgttga tcgccgcccg gaccacatag
     4201 accagcacat agcaaaacga aagcagccag atatacggat tcagcaacac atacttggtg
     4261 aggatctctt tacgcgtcag ccctgcccct tcttgttgtt gagcaatctc cagcgcgtcg
     4321 tgccgccatt caccaaccgc tggtaaacct aacgcctgcg ggcgatcgcg cagccgccag
     4381 cagagaaaaa tccccacgac gatcgccata caaccggcaa tcatcatccc ggcacgccag
     4441 ccgtaatgca gcgcgctcgc tgccatcaca atgggaatga gtgcgccgcc gacgttatgc
     4501 gccgtgttcc ataatgccca ccaaccgccg cgctcggtac gagaatacca ggcggttaac
     4561 agacgcgcac acaccggtga accccagccc tggaaaaagg cgttcaacac ccagagcacg
     4621 gcaaacgccc atagcgacgt cgagaagcca aacagaatgt tgataatgcc cgtggcaata
     4681 agccctatcc ccataaaata acgggcattt gagcgatcgc tgacaatgcc ggagacaaac
     4741 ttcgacacac cgtaggtaat gtaaaacagg gtcgctaaca ggccaatatc gctacggctg
     4801 agcacgccgt tagcaaggat ttctggtacg gcggcgttaa aacttttacg cgtgaagtaa
     4861 aacagcgcgt aacccagcca gatggtcagc agaatatgcc gacgccagta acggtaacgg
     4921 gcatcaattt cttgtttatc gctaatcaac ggcgcgtcag ctggcgcttt cagaaacggc
     4981 aacatcatcg aaccttagac ataacgttga ggtagagaaa cgctgacacg cgtgccgtgc
     5041 agacaggaaa tggtcaatgt gccgcccagc gccgttacgc gctcgcgcat tccggtaagg
     5101 ccaaaaccgt gttgcccgga atctggcggt aaaccgctgc catcgtcttc aatcaccagc
     5161 attaaccgct catcctgctg ccagccttgc agcgtgaccg cgctggcatc ggcatgtttc
     5221 acaatgttgt tcaacccttc ctggcagacg cgaaatagcg tcacgcgctg gttttcgctt
     5281 aacgctgatt catcgattcg ccattcgaga tgactgacaa tgccgcgccc ttccagctcc
     5341 atttcccgca tcagtgagcg gatggcctgc tccagggtga gatcatccag ctggcgcgga
     5401 cgtaaccgcc ctaacaaacg gcgcaccgca tcgtaaacac ccagcgacag gtgttcgatg
     5461 agctgcccgc tctgcttcac gctggcgtta tctgccgcca gccgctgaac aatacctgct
     5521 tgagtacgaa tagcagtgat ggtctgaccg atatcgtcgt gcagctcacg cgccacatca
     5581 cggcgcacgc tctcttcagt ttctaacaaa cgttcagcca gatgctgatt gcgcgctagt
     5641 tccttttgca gcgactggtt aagttcacgc aaccgctgga tgcctgcgcc cagtaacaac
     5701 cctgtcagac tttgcaccag cagcgagagc aataaatcca ccgaatgatc gcgccaggtt
     5761 tgactggcga tcagcgcgat ggcgttcatc aacgtcgcaa tcagcgcccc ttgccagccg
     5821 tagtgccagg ccagcgcgat aatcggcagc gccaggcaga atggcgtaaa gcgcgacagt
     5881 tcgtccggca atcccaactg gagccagaga ctgataacaa acagcagcaa gtaccagacc
     5941 agatgccgcc cgcgccagtt gatcggttga gaaactaacg acggcccgag cggcagccag
     6001 atgttattgg cgagatagtg ccagaacacc agacatatcg gggccagcgt caggccgcca
     6061 gttaaagtca gcaacagcgc attccacgac tctttcccgt gccaaagcca gggcagcgac
     6121 tgcaacaacg ccgccgccgt cagtgctgcc ccctgtaaca gcagggtgcg ccagtcacgt
     6181 tgatggcgat agcgcgagat aagcgccacg ggcaataacg tcagtaaact accgatcatc
     6241 aataaggaga aatgggttaa accgaccgcc tgcgttagcc agtaaatcag cagccactcc
     6301 gctcccagca gcaccggcca gtagccgcgc gggcattgca gcattagccc cagacgcaga
     6361 ccaaacggaa ataacagcac cgccatatca gggcgctcaa ccaggtgcag gctgatactc
     6421 cacaagcaaa accacgcggc agagaagata aaaaagcagg caataacggt aattaagcga
     6481 gagaaaaacg tcttcatcac cagccatcaa acatgcggcg cgccagttca acgtcgttac
     6541 tgacgcccag tttttccatc aggttagcgc gatggacgtg taccgttttc ggtgacaagc
     6601 ccagttcggc agcaatctct ttcaccgcca ttccttgcgc cagtttttct gccacctgcc
     6661 gttcacgttt ggtcagcggg tcctggcgac cagatgccag tttaatagca atatccggcg
     6721 tcagataaca gccgcccgtg gcaaccgtat gcaccgcagc aatcagttcg tcagggctac
     6781 aacgcttgga gagaaagccg cgcgcccccg cgttaagcgc ctgctcaacc agcgccggac
     6841 tgtcatgaac agagagcata atcgtcgcca tacctttcgg cagctggctt agcagctcca
     6901 gaccggagat atcgggcatg gagatatcgc aaatacacac ctgcacaccg cgccccggca
     6961 gccccgccaa cgcctcgcgc cccgaaccaa actcggcaac tacctgcaaa tcaggttcca
     7021 gccccagcag ctgcgcaaag ccggagcgga cgatgaggtg atcgtctata agtgcaacgg
     7081 tgatcatgat tgggtcctgg cggataaaaa acgcgcatac cttaacgata agcgcgatgt
     7141 tgttcaagtt ggatatgggg tatgtattgg cttaatgatg ttggtcttat cgtcaaaacg
     7201 cttacctgaa aggtaagcgt ttggtacgtg ttattgcgaa aaagcgaatt atttgtaggt
     7261 tgcgattgca atattgcctg aaaaatttct cctcctgttg cggaaatcgt tttcaggtaa
     7321 caaagagtca tccagaaacc tataatcttc agcggaacgc tcattaagag agtcatccac
     7381 tgagtcgtta tgaatataat cctcaccgta tgttcgataa atgaaattag caatattaaa
     7441 atcgttacca gcttcattta tttcggcttt tacaatataa tgggtttgtg tgaatgtatg
     7501 ctgtgaaaag tcagcaacaa gcaaatttgt ttttcccagc aaagcacaaa accctgggtt
     7561 atcatcaagc ccggcagaaa cagaatcgtt tgcctgtatt acattaatat cccccctatc
     7621 aagcgcgaca ggaaatgcct gtgaacaagc ataatacagg ttacctattc ctgaaatact
     7681 ggagaggcaa gaaataatat tttctctgat ttttttctca acaccatctt tctgtagaga
     7741 gtttctcaca atattacatt catctggcgt aaatacaatt tcactaaaaa gcttttcgaa
     7801 agctttatat tttctaacac ctccatggcc agacaaagcc atggttttat tcctcagaaa
     7861 ttcatggatt gtgctattta ttttaactcc acaacatgaa ataattgagc tgccctctcc
     7921 aacgtgtctt gctgcatcag caagaagtga actaaactct ccctccagaa catctagttt
     7981 tttatgtctg atttataact acaatcatct ggaattaatt tatctggagt taattgagtt
     8041 tgttcattta acgctttttt taattggcgc tcagcatcag taactccaaa actgcgttta
     8101 ttttcagaat tattattaac aacttctagc ttaagagcat ttgcagcaac tgttactttt
     8161 gagagttgat acttttccaa tacatcgtgc tctgataata accctataaa ctctttcaca
     8221 ttacgaccac taaaatccct gtctctactc tgttttttca aactgttgcc ttttaactca
     8281 cgatacacat cagtgacttt aaacttatca ctgccggtct gctcaatttt tatatagcta
     8341 tccatccctg tggtagcgat ccatgtttta taattcgcat tcgctgattc aggcgtaaca
     8401 tgaaaataag aaaccaattt accactgata tcatgccgat agatatgtaa agaatctaaa
     8461 atgtataata ataaacttcg gaaaacctca cgaaaagtgg catttttaga taatgttcta
     8521 caattatcga gcaggccact ttgttccatc gcatcaaatg cttcaatatt accctcgata
     8581 ctatctctta gactctggct caggccctgg ctctgagttt gactctgaaa atgactatta
     8641 tcattctctg tttccgttgg atatgtataa ctcgtcacac taaccatagt aacctcgcaa
     8701 tatgacactt tgcatatttt gacattgtta gaagtatcaa tggctagcat gataaatatt
     8761 tcattgctaa ccgctatttt tcatttattt caataataag actatagtgc cgttaatttt
     8821 tatcgatttc cccaatgcaa ttgcatcact aattctactt attcactatc aaaaatcgct
     8881 catttttttc aaaaagagtg tttacacaca gacggttaag aataagcgtt caccttaatg
     8941 ataaacgcga tttatcagac aaaaatcata aatcagcagt tgttgcggca ccgaaaaatg
     9001 taaaagcgac tttatcgttt ttggagtttt gttcgtcgac cctaccggta gtaaaataac
     9061 gagaacactg aagcttaact tcatttccaa cattattaac cgccatcaaa tacatcttct
     9121 gactaaaccc atttatagta aaatccattt cattaagata ctttccagac ccaaaaaaag
     9181 aatttcctga tttttttaaa tccgttgtaa atgaaatgaa actatcaaaa ctctttgaga
     9241 gtaactcgcg aacatcttga gatgtgtgtt gtaagcgact ctgatagcct tgtgcggaag
     9301 aataataaaa acatgtaatc ccctgaggac tggctaagca agcgattatt tcatcaatct
     9361 tatcatccgt aatattaccg tttgattgtt ttaataattc tcttacccga gcacaatcat
     9421 catcattaaa gcaaacctgt gcaaccaatt gttcaggtgt attttgacta tagtttgtgt
     9481 ctttttcaat tatttcaatt ttgcctgcaa atattttaag aatatcttta cttaactcaa
     9541 caccacagca agttattttt gcagcaccac ttggtttagt ttgttctttt atacatctat
     9601 gagcatccat agtcatagag tgtaaataat cctcagtaat atcaattttt tcatgaacag
     9661 gtaaatgccc ctcatctttt tgaattcgca ggtttaataa atcatatgta ttactcaaaa
     9721 tttccgcagg catttcgtaa ttacaatccg tattcatcat aacaatacta ttaactaatt
     9781 ttttcctcaa aaccgtgtca gcagaatgtg tttctaacac agccgttttt tcagatagcg
     9841 atttgataat agtcgctaca gccactccag aatatgatgt ttcttcgagt attatttttt
     9901 tatttttttc acctgcgcca aactgtataa cattaaagga tttgtcacct gtttgttcaa
     9961 tcacggtata ttcgttctct ccagttgccg ccatccaggt acgattacca ttattttccg
    10021 tgcctggcat agggtggaaa tagtcaagaa tttcggtatt aacactgcga gaaccggaca
    10081 tgatatttag tattaagcgc tttatttcct gtaacatcca ttttgcgaaa ttactgccag
    10141 gtgaatattt tttctcgttt tttgtaatat caagctcagc agcgatattt atatttttat
    10201 ccaaaaaagt ggaagggcag ggatttgaca tcattgttcc gctgattata gggcttgcca
    10261 taatatacct cacgatgaac cattaattaa tagaatgctg gtcatgacta ttggtataga
    10321 cagcaatcag aatttaacac catcgctgga aattttagcc ctaactgtta acccccaaat
    10381 taccggtgca gcgacttaaa ttaacattca gggctaataa attgtgcctg agatggtctc
    10441 tggcacaatt tcaccctata tatctgaacg gatctatcaa aaatcgctca tcagtacccc
    10501 cccgggcggt ttaatgtcgc tccttactga aaaaacaccg cgatcttgtt aaacattgtc
    10561 ggatcagact gattacgctg cactttcacc acatcttcca gcttatcgat ttggcttatc
    10621 atctgctcca gacgctggtc gtcattgacc agtagccaga tgtggctttt gtcgctgtcc
    10681 tgaatcggca ggcagagaat gccttcaacg ttaaaagcgc gacgggcaaa aaggccacaa
    10741 acgtgggtca ttacgcccgg atggttgcga acggtgagtt ccagaatcac gttgtcatga
    10801 gttgtgtttt gcatggctta ttcccccacc atttcagtat tcgccgcacc tggcggcacc
    10861 atcgggtaca ctttttcttc ggcatcaata cgcacatgga tcagcgccgg accagggcga
    10921 ttgatgattt cctgtaatgc agcctgcgga tcggtttcgt tattcaaatc acaggtttcg
    10981 aggccgaatc cggcggcaat ctgcataaag ttgatttttc ccggataggt agcggcaaaa
    11041 acgccctgct tgtagaacag actctgttgc tgatgtacca gccccagcgc ttcgttgttc
    11101 atcagaatga ttttgacatc cagctgattt tcactggcgg tcgccatctc ctgaatattc
    11161 atcatcaggc tgccgtcgcc ggagaaacac aacactttgc gatccgggtt cgccagcgcc
    11221 gcgccaatcg ccgcaggcag gccaaagccc atcgtgccca gcccgccgga ggtcagccac
    11281 tggcgcgggc gattgagcgg ataggcttgc gcggtccaca tctgatgctg acccacgtcg
    11341 gtggtgataa tcgcattgtc atcgacacag gcggcaacag cgttgatcag gccgtaatgc
    11401 gttaacggat cgcacgcttt cgggattgga cacgggaatt cgcgctgcaa atccgccacc
    11461 aactggtgcc actctgcacg cggttgcgct tccacctgcg ggatcaattg cgccagcacg
    11521 tcatcaacat ccgcctgaat cgctacgtgc ggttgcttga ttttgcccag ctctgcacgg
    11581 tcgatatcga catgaatgat tttcgcattc gggcagaact gctcggtttt gccaatcgcc
    11641 cggtcatcaa aacgcgcacc gagaacaatc agcagatccg cctcctgcag tatatagttg
    11701 gtgctgcgca cgccgtgcat ccccagcata cccagcgaca gcggatgcgc ttttggcagc
    11761 atgcccagcg ccattaaggt catggtagtt ggcaattgcg ctttctctgc cagttcacgc
    11821 acccgtgcgg gcgcattgat cacaccgccg cccagataaa gcaccgggcg tttggcggcg
    11881 ttaatcattg cagctgcgtc acgaatactc tcttcactaa acgcaggcgc ggcagctttt
    11941 tctgccatag cggcctgtgc ttcaatctca aaaaccgccg tttgcacatc cttaggaatg
    12001 tctatccaca ccgggccagg gcggcctgat tgcgcaatac gaaaggcgtc gctcatgacc
    12061 tgtgggagct cttcgatatg tctgaccaga tagttgtgtt tggtgatggg gatagagatg
    12121 ccgtaggtgt ccacttcctg gaaggcgtcg gtgccgatca tcgaggcagg aacctgaccg
    12181 gtgatgcaaa tcagcgggat ggaatccagc cgcgcatcgg caatggcggt caccaggtta
    12241 gtcgcacccg gtccgctaca ggccatacag accgccggtt taccgtcggt gcgcgccatt
    12301 ccctgagcga taaaccccgc tccctgttca tggcgagcca gaatatggcg gatttgcgtg
    12361 ctttggctta aggcatcgta aacaggcagg atagaaccgc ccggaatgcc cgtcacaatc
    12421 ttaatgccct gctgttccag gaaatgaacg ataaattctg cgccggtaaa gcgcttacgc
    12481 gtcgatgttg tgcccgaact tgccatgctc cagtcctttt cttctgggcc gactttccgg
    12541 gaggtgctta aacgaaaaac cccgcccggt ttgcgccggc ggggttttgg aatcgtgtgt
    12601 tgttccagtc cctacggcgc attgccgacg accaccacca cacgcacgac gaccactgcg
    12661 gcggatggcg cagttggtag tagttttgcg ttgaacatgg aagtagtcat tggggacctt
    12721 gttggttttg tgtttaacaa tatttataca agcacagctt tacaggggag acaatggaaa
    12781 atttttcagc aagggaaaat tgaggggttg atcacgtttt gtactgaatt gcgggtaaca
    12841 aaaaaccccg ccggagcgag gtttcgtcag tcgcctgcgg ctggtaaccg caaagcacac
    12901 tgtattatgt caacactgaa agtatacgtg ttccgcgcag aacgcgcaat ttcggcacga
    12961 attttgacgt atttagtaca tagttgagta tcgatcacag ttttcgtttt gtccaaatat
    13021 tactgtttat ttatacagta aacttctata atatcactgt acgcaatgtg ttatgcgggg
    13081 gccgcatcgt tacccggcgc actaagtcct ggctgaaacg ggtggtgccg tcagcgcctt
    13141 aaccccgcgt gagcacactg tgttatgtca acaagcacaa cgtttctcct tgagataccg
    13201 cgtgcacaac agctggcaac aggcagcgga aaggtacgtc agctggcagt gctcctgaac
    13261 cacaggagac gcgtatgagc ctggtggata tcgccattct tatcctcaaa ctcattgttg
    13321 cagcactgca actgcttgat gctgttctga aatacctgaa gtaattcaga ttcaagtcgc
    13381 accaaagggg agcgggtaac cgctcccctt tcatatttag cgtgcgggtt ggtgtcggat
    13441 gcaatgctga tgcatcttat ccgccctacc atccctcccg gcaacattta ttgccgcttt
    13501 tgtttacata ttctgccgct aaacaattcc ccattcctgg cgtatatctg gctaacattt
    13561 atcaatgtga tagattcctc tcccgcattt atgggaatgc gtagtgactt attctaatta
    13621 tttttataaa agcatccgtg ataatgaaaa ggcatagaaa cgtcaatttg ttattgatgt
    13681 tggtattact cgtggccgtc ggtcagatgg cgcaaaccat ttatattcca gctattgccg
    13741 atatggcgcg cgatctcaac gtccgtgaag gggcggtgca gagcgtaatg ggcgcttatc
    13801 tgctgactta cggtgtctca cagctgtttt atggcccgat ttccgaccgt gtgggtcgcc
    13861 gaccggtgat cctcgtcgga atgtccattt ttatgctggc aacgctggtc gcggtcacga
    13921 cctccagttt gacagtattg attgccgcca gcgcgatgca ggggatgggc accggcgttg
    13981 gcggcgtaat ggcgcgtact ttgccgcgcg atttatatga acggacacag ttgcgccatg
    14041 ctaatagcct gttaaacatg ggaattcttg tcagtccgtt gctcgcaccg ctaatcggcg
    14101 gtctgctgga tacgatgtgg aactggcgcg cctgttatct ctttttgttg gtactttgtg
    14161 ccggtgtgac cttcagtatg gcccgctgga tgccggaaac gcgtccggtc gacgcaccgc
    14221 gcacgcgcct gcttaccagt tataaaacgc ttttcggtaa cagcggtttt aactgttatt
    14281 tgctgatgct gattggcggt ctggccggga ttgccgcctt tgaagcctgc tccggcgtgc
    14341 tgatgggcgc ggtgttaggg ctgagcagta tgacggtcag tattttgttt attctgccga
    14401 ttccggcagc gttttttggc gcatggtttg ccggacgtcc caataaacgc ttctccacgt
    14461 taatgtggca gtcggttatc tgctgcctgc tggctggctt gctaatgtgg atccccgact
    14521 ggtttggcgt gatgaatgtc tggacgctgc tcgttcccgc cgcgctgttc tttttcggtg
    14581 ccgggatgct gtttccgctg gcgaccagcg gcgcgatgga gccgttccct ttcctggcgg
    14641 gcacggctgg cgcgctggtc ggcggtctac aaaacattgg ttccggcgtg ctggcgtcgc
    14701 tctctgcgat gttgccgcaa accggtcagg gcagcctggg gttgttgatg accttaatgg
    14761 gattgttgat cgtgctgtgc tggctaccgc tggcgacgcg gatgtcgcat caggggcagc
    14821 ccgtttaagc tcacgtcacc gcagcatcgt catcagctcc atgggagaac gatgctgctt
    14881 tatcagatca cgcatcaccc gcatatgcgg cgcggagtaa gaataaaacg cctgatagcc
    14941 cgcacaaagc acgttgtctg gcgtctcctc ccgagcgtgc gggcacaggc gatgccagtc
    15001 gcacgcctgg cagccacggc gcggttgcgg gttttcgctg ccacaccatt tttgtagggt
    15061 ggcgtcaaaa agctcaatcg aaatacgact gatatctgcc cgtacccaac actcgaacag
    15121 ggcaattaaa aatttccccc acagctctgc tgtcaccgaa gcggccgtca gatcgccccg
    15181 gtcatcaaat tcaagatggg gaataagctg tacggagcgg ataccccgtt gtttgaaatt
    15241 gtgataaaaa tcggcaggtt gcagagcatc cttgtactct acaaccagtt tatgcctgtc
    15301 ctcttcagca tcaatgacct gacttcctgt catgcgactc tctcaataaa tacgataagt
    15361 tgatgtatat gcgttacagc aaacagtatt gcgagagata acaccgcgag ggagatcaaa
    15421 aatttgtcac ggactacgtg aaattgggaa aaatcgctat tcgtgacatc cattaaattg
    15481 cgattacggg tgtagtgcca gaggatcagc gccacaatgg cgagaatgcc aatcgaaatc
    15541 cagaataaca tgcccgcctg gtgccagttg tgtttgatag ccaacgccat caacgcgccg
    15601 tagcccagca tggtacgaaa ccacgccagt gatgtacgct ccggctgcag ccctgggtcg
    15661 gcaatgcgtc tggctttgcg gctatccggc atacaacacc agtcccataa cgatcaccgc
    15721 tacgaccatc aatattaagc tgatgattaa taagctgttg gtgtagggca ggtcctcttt
    15781 cagacgcatc gctttttcat tgcgtaacca gcgcaggtag ccatacatcg ctaacccgcc
    15841 ggaaaacaga cacaacaaca acgccagcag ttcgcgaatg acgggcgtgg cgaaatctgg
    15901 cgcaagttga tcaagcccaa cccccgccgc cagaaatcct agtgcggtac ggatccacgc
    15961 cagaaaagta cgctcattag ccagcgagaa gcggtaatcg ggcgcttctc cgaggcggga
    16021 aattttcatt gttgctcctt gtaaactgtt cctcgccgaa aaagtaggcc aggaacagca
    16081 ataatgcatc agaacttctg ttttttctct tccaccttca ccccttgtgt cggtaggccg
    16141 gtgtcatagt cgcgtaccac tggcgaatag ccatcttgtg ggcgtggacg aaacgccccc
    16201 atccagcgcg gtcgtgcatc tttacgccac ggacgcagac tccattggta actgcggaac
    16261 ggatcgcgaa ttttatccat gtaatccaat aaggcgtcat gcattttgcg gcgaacgtcg
    16321 gcaaaacgga tatcatcgat cagattatgc atttcattta ggtcattacg gcgatcgtaa
    16381 agttcatcac tggtgaagag gttgagtacc agtttaaagt catccgtcac ccagcaacgc
    16441 accggaataa aaccgccaaa gctgtcatgc tcaatctcgt agcggttaaa ttccaccatt
    16501 acgccgcgtg gctctttcac ggcaaggata ttttcccccg gcagaatctc tggtttttca
    16561 atatctgcca gcgccatcat tgtcggcagt aaatcgatat gactgactgg cgtatcgacc
    16621 tgccgccgct ccccttgcgg cgaacggatg atcagcggaa tacgggtgat gtcgtcatac
    16681 atcgccgctc ctttactgat caacttatgt gcgcccatca tttcaccgtg atcggaggta
    16741 taaatgaccc acgtattttc acgttgctct ggcgttaagg cattgatgac ccgtccgatt
    16801 tggtcatcaa caaagtcatt gcaggcaaaa tagagcggat ggtgataaag cccgtcatca
    16861 ccgactggcg atggcatcgc ctgcgcccat aagcggtgat gttccggttt gttcgccagg
    16921 tcatcctgtg atttctcgcc aagttcgtag taaaaatcag cgtatttctc taaatactcc
    16981 accggacagg tgaacgggtg atgcggctca tcatacgaaa ccaccatcag gaagggttcc
    17041 tcggcgcgcg cgggctgttg cagaaaatct accgcccgat tgctgatgcg atgcgcccag
    17101 gtgaaggttt cgtcgatatg gttcgcctgt aaatcctcaa cgctgtttag gccattgcgc
    17161 cacaggctga tctctttttc cgtcagttcg ctaaggtagt tcgccccatc gaaccagtaa
    17221 tcagcgtccc actccggcgg acactcgcca gtgccgaaat agtcatgacc gtcgagatgc
    17281 catttgccga tgtaacaggt gtgatagccc gcatccttaa agtagcgtcc catagtggag
    17341 atgtttttgc ccggcgcgac gttgttggtc cacgggccgg actggttagc gtagataccg
    17401 gtaaacagcc cggcgcgcgc tggtgtacaa accggtgaac aggtataggc ggaattaaag
    17461 cgaatacttt ccgccgccag actatcaata ttttgcgtat tcagcggctt accgctatag
    17521 caaccgacca tattggtggc ctgggtatcg gtcatgatga acagaaaatt ggggcgtttc
    17581 ataggctatc cttcgcgtcg ggagtaaagg ttacggctgg gtcgtgacga tgccgccagc
    17641 tgtcaaaaat caggtagaca atcactacgg cagcgatgaa ataactgatc atcgctaacc
    17701 agcgcgtacc gtagccgccg aattcagcca gcccggcata gacgccaatc atagcgaaca
    17761 ggatgccaat tgacgcgatc ttgacgtttt tccacggttt catgtccacc gcaaacgcat
    17821 ctttgaaggt gaacggcgtg gcgcgcggtt tgataaaacc gatcaccagc atcacgacca
    17881 cgttgatgca gaacgtacag gccagcacat agaggaaatg gaagtcgaac ttcaccagat
    17941 agttgatggt gatgtagctg attatgccaa tccccatcgc cacttttgcc gccagcgccg
    18001 ggatgcgcgg gaagaaaaag cccataatga tgatggtaac cagcggcacg ttgtaaatac
    18061 cgttgagctg tttcatccag ctatacagcc cctgcggcgc gttggcgatc cacggcgcga
    18121 ccagcaccga aacgatggcg ataaagaaac caaatttgcg cccaacggtg accagctgct
    18181 gcggctcggc attctggtta atgatgcgcc ggtaaatacc catactgaat aacgtactgg
    18241 cgctattcag aaagccgttg aaggtactga tcaccgcacc aaataacacc gcgccgaaga
    18301 aacccaccag tggcactggc agaacgttat tgaccagcgt cgggtaggcc atgtcggctt
    18361 taggtaaatc ctgatacaga tgaaacgcaa tcaaccctgg cagcaccagt accagcgggt
    18421 cgagcatttt cagcaccgcc gttaacagcg cccccttttg cccttccgcc aggctttttg
    18481 acgccagcgt gcgttgcacg atgccctgat ttgtacacca gtaaaaggtg ttcaccagaa
    18541 tcaaaccggt aaatgccgcg ccaatcggca agggatcggt cgggccaccg actgagttta
    18601 atttctcggc gtgaacggtg gtgagttgct caatgccctg cataaagctg cccttgccca
    18661 tcgcgatcag gccaaatatc ggcaccatca acccgccaat aaccagccca ataccgttga
    18721 tggagtccgc cactgccatt gcgcgcagtc cgccgatcac cgcatacaaa attcccgcca
    18781 gaccaagcaa aattaccaat agccagatag ccgcaccgtg ggaaatttgt aacgattccc
    18841 cgacgtgaaa caggctgttg agcgccaacg cgccggagta gagaacaatc ggcagaaagc
    18901 agacaccggt ggcaatgagg aagcagaagt cgatgataat acgcgtcgtt ttatcataac
    18961 gttcctccag gaaatcgggg atggtggcaa tgccacgctt cagatagcgc ggtaaaaaga
    19021 ttagcgcgag gaagatcagc gtcaccgcag aagtgacttc ccagcccatc accgacatgc
    19081 cgcttttgta ggcctgcccg gaaagtccaa ccagttgttc cgtggaaagg ttggttagca
    19141 ttaacgaagc ggcaataacc ggcgctttta gtgaacgtcc ggcaagaaaa tagccttgtt
    19201 gcgatccggt atctgttttg cgaaccttcc accaggtgat gatcgccacc agcagcgtaa
    19261 aaccgacaaa actcaagatt tgtaacgaat tcatctcata gcccttattt atgtttatgt
    19321 cccggacgca tgaatatagg gagaggtaaa atgcgtcggg tcaggcagtc agattcttaa
    19381 aatacaaacg tcgtatccct gaacggattg ttttctgtta agttcaggtt gtgagcataa
    19441 ttcgccggag ggatattaaa atgaatggaa aattgcaaag ttcggatgta aaaaacgaaa
    19501 ctccgtacaa tattccctta ttgattaacg aaaatgtgat ctccagcgga atttctctga
    19561 tctcgctctg gcatacctac gccgacgagc attaccgggt gatctggccg cgggacaaga
    19621 agaaaccgct tatcgccaac tcatgggttg cggtgtatac cgtacaagga tgtgggaaaa
    19681 ttattttaaa gaatggtgaa caaataacac tgcatggcaa ctgtattata tttttaaagc
    19741 caatggatat tcactcttat cactgtgaag gtttagtatg ggaacagtac tggatggaat
    19801 ttaccccaac cagtatgatg gatattcccg ttggtcagca aagcgttatt tataatggcg
    19861 aaatttataa tcaggaactc accgaagttg ctgagttaat aacttcacca gaagcaataa
    19921 aaaataatct ggcagtcgct tttctgacga aaattattta tcagtggatt tgtcttatgt
    19981 acgcagacgg taaaaaagat ccacaacggc ggcaaattga aaatttaatt gccactttac
    20041 atgccagtct gcaacaacgc tggagcgtag ctgatatggc tgccacgatc ccctgtagcg
    20101 aagcctggtt gcgtcgtctg tttttacgct ataccggcaa gacgccgaaa gaatattacc
    20161 tcgatgcgcg tctggatctg gcgctatcgc tattaaaaca acaaggaaac tcggttggcg
    20221 aagtcgctga tacgctcaac ttcttcgact cctttcattt cagcaaagcc tttaaaaata
    20281 aatttggtta tgcgccgtca gctgtgctga agaatacgga ccagcaccca acggatgcca
    20341 gtccacacaa ttaacgtaac tctggccagt acgctttgtt ggcctccagc aactcatcca
    20401 gaatagcttt ggcgaccgcg gcgctcggta ccgttttcga caacgtaatc gcctgccaca
    20461 ggtgctgata tgaacgctgc tcccaggcgt ccacgaccag tttttccacc gccacttgct
    20521 gactcattaa ccctttctga aactgcggga tctcgccgac caccagtggt tctggtccgt
    20581 tgtgcccgac cagacacggg atctcgacca tcgcttcatc atcaaagtta tgaattgccc
    20641 cgttgttagg cacaatcagc aacatccgct cctgagtgtt gaaggcaatt gccgccgcca
    20701 gatcgacgat gtatgacgca tgttcgtcaa tttccagctt gcccgctgct gaatttcctg
    20761 ccgccgtaat ggcgcggcaa gcatcgaaaa cctgtttctc gcgatgctcc atcacctcat
    20821 tcgcgcgggt atgttcaggg ttggaatgct gaacgacata atccgggaag agataatatt
    20881 tcagataggt gttcggcaat gtatccgggt ccagtgcctg gacatcccgc gcttttgcat
    20941 aggtgtcgtt ccagctcgct tcagtatgtg tatcttgctg cggtggaaca taaccgtgct
    21001 tagagacata ttgccgcagc tggggcatca ggtcgttgcc ctgcaaatct tcaattgatg
    21061 tccaccagcc aaagtggttc aggccgtagt agcgcacgcg catctgtttg cgatcttgca
    21121 gcccaacaat ttgcgccatc cggctttcaa taccgattgg catgtcacag atgttgagga
    21181 ttttcgcatt cgggcgcaga cgacgcgtgg cttctgcgac aatggctgcc gggttggagt
    21241 agttgagcat ccaggcattt ggtgaatatt tttccatata atccaccagt tccaggacgc
    21301 cgccaatgga acgcatgccg tacgctattc cgccgggtcc gcatgtttcc tgaccaacaa
    21361 cgccgtggcg cagcgggatt ttttcatcca gttcgcgcat cgggtatttg ccgacgcgga
    21421 tgtgcgccat gacaaaatca acgtcgctga atgccacttc aggatcgttg gtgtaactaa
    21481 acgcaatgtc cggcgctttt tctttaagga tgactttaca ggcttcggca atcacttcct
    21541 gccgcgcacc atcgttatca taaaatttca gtgcacgaag cgggaaacgg tcctgattcg
    21601 ccaggagcat caacacgatg cctggcgtaa aggtgcttcc accgcctgcg acaaccactg
    21661 agaatttggt cattatactg cctccgtaat ggcaacattt tctgctgatt gatgagaatt
    21721 aattaagcta tcgagctgtt cacgcagctg ggatacatgc aaaccaatga tcacctgaat
    21781 ggcatcgcca ctacggaaga cgccgtgcgc tcccagcttt ttaaagactt cgtcatccag
    21841 cgtttgtgac atgtcatgca gtgcaatacg taaacgcgtc gcgcaattgt tgatgctgga
    21901 gatattgccg accccgccca gggcttgcag gataccggca gcctgatcca gctctttttt
    21961 tggctctgcc gcggtggttt ggcctcgcga ggctttgtat tcggcttttg agtagagttt
    22021 cacttccgca tcttcacgtc ccggcgtgca catgttgaat tgcagaatca gggtgcggaa
    22081 aaccacgaag tacagcaggc taaagcacaa cccaatggcg atttgggtca aaatcatatc
    22141 cgcgtggttg ctgaacatcg ggatccagtt ttgcggtaaa acctggtcaa tcagacctcc
    22201 gcccatgttg cccaccacac caaagaggta catcacggtc gacattgagg ccgcaagcac
    22261 agcgtgtacc gcaaacagca acggtgaaat gaacaggaag gtaaattcca gcggttcggt
    22321 aattcccacc agcatggcgg ttaaggttgc ggggatcagc aagcccgcca cttttacccg
    22381 attttccggt gcggcagtga agtacatcgc taaagaaatg cccacggcac caaagatttt
    22441 tgagttaccg tgcagggcaa aaccgccttc cgggaacaac gatttcagcg gctcggcgct
    22501 caaactgaac tcttgcagat gctgcgccca gtacatctga atgccgcctt caacagctgc
    22561 cggaccaaag ataaacggtc cgtagatgaa gtggtgtaaa ccggttggga tcagaatacg
    22621 ttcgaggaag gtgtaaaccc agaccccaag cgcacccgcc gaacgcagga acgcttgcag
    22681 agattcaatc cccatttgta cttttggcca gccgagcaac gtcagccagg cgcaggggat
    22741 catcaccagg aaggcgataa tcaccacata agatgtcccc tggaaaatgc cgagaaacac
    22801 cggcagtttt ttatcgaaca gacggttatg cagcgccgtc acaatgccgg aaatgataat
    22861 tgcgccgata atgctggtat cgagggtttt aatcccggcc atcattgtca gaccgctacc
    22921 tgccaccgcg tcctgagtga aatcgacgcc gaagtagctt ccccaggtca ttcccatcgc
    22981 gttgatgaaa tagttccagg tcaggaaact caccatcacc gccagacaag ctcgcccctg
    23041 cgcttgctta gcaaggccaa tgggtaaacc gacagcaaaa atcagcggca tattacggaa
    23101 taccgtccaa ccgccctctt caataatatg tacgatttgc gcgaataaac tgttcggatc
    23161 ggtcagtgat tccccgacaa acatcgggtt ttgcagcaag atggcaagac ccaccacaat
    23221 cccggcgaag ggaaacagca gcactggcgt gaacatcgcg ccgccaaagc gttgaatttg
    23281 actgagcatt tccacatcct cttttctcaa ttctgtaagt aggaagcctg attggttatg
    23341 cggcaggcct ggagcgagga taggaagatg cgatcggcag aacatgacga cccaatggga
    23401 ttcgtgatcg ctttcatgct tttcgttggg cggtagagat ctacgttttc gcaaaaaagt
    23461 agacatctgt gagcactggt acacagcaac gaacacatct ttaaaaaaaa gatgtttttt
    23521 caatcgatta agcagaactt gtgggcgcat tacccgggct tgcaggcaaa aaagagatct
    23581 agagatgatc tacaaaagca ttgcggagcg gttaagaatt cgacttaatt ccgcagattt
    23641 cacgctaaac agccttcttc ccggtgaaaa aaagctggcg gaagagtttg cggtatcgcg
    23701 gatgaccatc cgtaaagcca ttgatctgct ggtagcgtgg gggctggtgg tccgccgcca
    23761 cggtagcggt acttacctgg tgcgcaaaga tgtattgcat caaaccgcca gcctgaccgg
    23821 gctggtggag gtgttaaaac ggcagggaaa aacggtcacc agccaggtgc tgatttttga
    23881 aatcatgcct gcgcctccgg ccattgccag ccagttacgg attcaaatca acgagcagat
    23941 ctacttctcc cgtcgcgttc gttttgtgga agggaaaccg ctgatgctgg aagacagcta
    24001 tatgccggta aaactgttcc gtaacctttc gctgcaacat ctggaagggt cgaagtttga
    24061 atatattgaa caagagtgcg ggattctgat tggcggtaat tatgaaagcc tgacgccggt
    24121 gctcgccgat agactgctgg cgcggcaaat gaaggtagcg gaacacacgc cactgctgcg
    24181 gatcacctcg ttgtcctata gcgagagcgg ggagtttttg aattattcag tgatgttcag
    24241 aaatgccagc gaataccagg tggagtacca tttacggcga ctccacccgg agaagagtta
    24301 accgatactc cagaagagca ccgccagtaa ttggggggtg ataatgcgca ggaacatcac
    24361 taacggatag acagtggcgt aagagagcgc cgctgcaccg ctcgttggat gaagattatt
    24421 agcaaatgcc agcgccggtg gatcggtcat ggagccagcc agcatcccgc acatggtcag
    24481 gtaattcatt ttggctaaca tccgcgccag aataccaaca gtaatcaacg gaacggcggt
    24541 gatcagggca ccatatccaa tccagcttag cccttcgcca ttgaccaggg tatttacaaa
    24601 atccccacca gatttcagcc ccactaccga gaggaacagt acgatcccca gctcccgcag
    24661 cgcgaggttg gcgcttggcg gcataaacca gtacagcttg ccgatactgc cgatacgccc
    24721 gaggatcaac gccataatca gcgggccgcc tgccagtccc agtttcaacg cggccgggaa
    24781 tcccggcaca aagacgggaa tagaacctaa caatacgcca agcccgatac caataaacac
    24841 cggcaacatc tgaacctgtt gcagtttttg ctgcgcattc cccagcacat tggcaacggc
    24901 atcaattgcg gacggacgcc ccaccaggtt gaggatatcg ccgaactgca ggctgatatc
    24961 gccactggcg accagttcga ccccggcacg gttcaggcgc gagataacaa cgtcatagcg
    25021 ttctttaaag tgcaggtcgc gaatacgttt accgagcacg ttttcattgg tgaccaccac
    25081 acgctcgacg cgtaaatcag tgcctttcgt ggacaacgag gtatcgacct cctgaccaat
    25141 caccagttgc gcattatgta aatccgctgg ctgccccacc agatgcagca aatcgcccag
    25201 ttggataatg gtatctggcg aaggtacttt tagggtttct tcgcgtttca gacgcgagca
    25261 gataattttg tcgccgttga gaatcggtac atctttaatg gctaaatcat gcaggttagg
    25321 gttctcaacg cgaatattga tagtcctgat cagcgcgccg ccattggtgc gagaagactc
    25381 gtgctgctgg gcttctgtct cgacattgac gcggaaaata acccgcaaca tccacatggt
    25441 gaacaaaatc ccgcaaatac cgaatggata cgccatcgcg taactcatcc ccatctgatc
    25501 gaccatttcc attggtgtac ccaggtcgcg caagatctgc tgccctgccc ccagcgctgg
    25561 cgtattggtt accgcaccgg agaaaatccc cagcactacc ggcagtggaa tatcaaacag
    25621 tttatgcagg atggcggtaa ccagaccccc gatgatgaca atcagaacag caaacaggtt
    25681 gaggcgtaat ccggagacgc gcaatgaggc aaagaagccc ggccctacct gaatcccgat
    25741 ggtataaacg aacaggatca ggccaaattc ctgaataaca tgcagcatat cgctacttaa
    25801 tgtcatcccc gcctgagaaa caaaatggcc gacgatgatc ccaccaaaca gcacgccgcc
    25861 aatacctaat cctatgccgc gaaatttgac gttgccgata aacaaaccga cgactgccac
    25921 caaagccaga atactgaccg ttaatgctat atcactcatc gtccatttct cttgagataa
    25981 cattttaatt gctacggatt atggctgaat cggtgaagca gatttgcctt atagcgcaca
    26041 ttatcacggg aaagaacgtg ccgaaatatc ttaaacaggc gaaatttaac gcattgttga
    26101 agaaaaaaga caaaaaaagc cccaccagaa tggcggggca aagagaatag ctagttagct
    26161 atttaacgcg ggacgttcgc tgatagcgat acgctgcgct gcgatgggtt caggctcatt
    26221 acgaattaaa tcaatatgca gtaaaccgtt tacgaaggtt gcaccagaga cttccatatt
    26281 ttcagccagc gtaaagctca ggctaaatgg ctgattcata agcccttgat gcagccattt
    26341 tttctcttct tttggctgct ccggcgtgcc ttttacgctc aggcgcgtac cttccagttg
    26401 aatctctaaa tcttcctgac gaaaacctgc cagcgcaagg gtaatgcggt agtggttatc
    26461 gtcgcttttt tcaatgttgt acggcgggaa gctctggctt tcaccggcgt tttgcagtgc
    26521 gttggccagt ttgtcaaaac cgatccattg acgcatcagt ggggataaat cgaagttacg
    26581 catagtcatt tctccttcta agaagcgagt aagtacctgc aaatccgaag attcgcatat
    26641 gctccctgac ggcgagcatg gagatgtcag gccgcgccag gcggccttag ggaattagtt
    26701 gatttcgata cggcgcggtt ttttcgcttc cggaatcacg cgttcaagat cgatatacag
    26761 cagaccattc accaggttag caccacgaac atgaatgttc tcagctaact ggaatttgcg
    26821 ttcaaagttg cgttcagcga tgccctggta cagataggtg cgctcttttt gttcgtcggc
    26881 gtgagcacct ttcaccacca gcagattatc ctgggcggta atttccagtt cgctctcagc
    26941 aaaaccagcc acagcgatag caatgcggta atggttttcg tctaccagtt caacgttata
    27001 cggagggtag ccgccattac tctggctctg gttgttttct aagtggttaa acaaacggtc
    27061 aaatccaata gcagaacggt aaagcgggga taaatcaaag ttacgcataa tcaatagctc
    27121 ctgaaatcag cgagaatgta agaccttcca caatggacag gtcaggtagc cagaacaccc
    27181 atcaggcgcg tcctcatcgg ctacgatgta aaaatgggtc tggaaatgaa cttttcaagc
    27241 cttatcagcg gacttttttt tgcagtttat ggtctattgc atagactgag ggggcagcac
    27301 gaaacgttaa agtgcgatag ccgccacagt gcgacgtaat gatgatgtta ttttcagcaa
    27361 gggatcgcta taactcatca ggccaacacc cacgatcaac agatgaattt attatgataa
    27421 gaaatgtgtt gttagcgttc atgatatgca gcggaatgac attactcgga ggatgctcca
    27481 gcgtgatgtc ccataccggg gggaaagaag gaacgtatcc tggcacccgc gccagcgcaa
    27541 ctatgattgg cgacgatgaa accaactggg gcacaaaatc gctggcgata ctcgacatgc
    27601 catttaccgc agtactggac acaatattgc taccgtggga tgtgttccgc aaagatagct
    27661 cagtgcgctc gcgcgttgaa aaaagcgaag ccaacgcaca agccaccaat gcggttattc
    27721 caccggccag aatgcctgat aactgattta gcgatcagtt tcggtgatcc acagctggcc
    27781 accttccatc cacgccagcc attgaccatc cggcgagaag accacggcgt ccgcagaagg
    27841 tggattggcg tggttttcgg ttaaatactc aacctcgccg ctttgcgcat gggcgcaagc
    27901 aattcgatta tccagcacaa agcccaacca ttctcctgac ggatgccagt taaatgcaga
    27961 ctgaatatcc gttttgttat gggttaactg gcgcggctcg ccgccctgtg gcgagataag
    28021 ccacagttgc acaatgccgt tatcatcacg cattaaaaac gcgatttgcg taccctgcgg
    28081 attacagcgc acccagtggc gcgggacgtt gactaacccc ggataagccc gatggtgggt
    28141 aaaggttaaa cgtcgctgca cgacgccacg cggtggcgcg ggcagggttg tttccgttcc
    28201 acttaacggc gcatcacctg ccgctttcca gccagcttca tcttgcggta actcaacgat
    28261 aaatagctcc ggcactttct cgccctttgg cgaaagtgtg tcgccaataa acgccagcgc
    28321 gtgatttcct acccatcctt cttcataagc acgattgatt tcatcgctgc caggctgcgg
    28381 cgtgggcgtg gttttactca ccagcacgca ccagtggcta ccgctgtatt ca
//
LOCUS       DAMZFT010000057        28199 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.057, whole genome shotgun sequence.
ACCESSION   DAMZFT010000057 DAMZFT010000000
VERSION     DAMZFT010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 28199)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28199)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28199
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.057"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(<1..206)
                     /locus_tag="PN977_002552"
     CDS             complement(<1..206)
                     /locus_tag="PN977_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF945 family protein"
                     /protein_id="HDI6042571.1"
                     /translation="MNKSLVAVGVIVALGVVWTGGAWYTGKKIETHLEDMVAQANAQL
                     KLTAPESNLEVSYQNYHRGVFSSQL"
     gene            complement(307..1482)
                     /gene="manA"
                     /locus_tag="PN977_002553"
     CDS             complement(307..1482)
                     /gene="manA"
                     /locus_tag="PN977_002553"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase"
                     /protein_id="HDI6042572.1"
                     /translation="MQKLINSVQNYAWGSKTALTELYGMENPSSQPMAELWMGAHPKS
                     SSRVQNAAGDIVSLRDVIESDKATLLGDAVAKRFGELPFLFKVLCAAQPLSIQVHPNK
                     RNSEIGFAKENAAGIPMDAAERNYKDPNHKPELVFALTPFLAMNAFREFSEIVSLLQP
                     VAGAHPAIAHFLQQPDAERLSELFASLLNMQGEEKSRALAILKSALDSQQGEPWQTIR
                     LISEFYPDDSGLFSPLLLNVVKLNPGEAMFLFAETPHAYLQGVALEVMANSDNVLRAG
                     LTPKYIDIPELVANVKFEAKPANQLLTQPVKQGAELDFPIPVDDFAFSLHDLSDKETT
                     ISQQSAAILFCVEGDATLCKGSQQLQLKPGESAFIAANESPVTVKGHGRLARVYNKL"
     gene            1681..3327
                     /gene="fumB"
                     /locus_tag="PN977_002554"
     CDS             1681..3327
                     /gene="fumB"
                     /locus_tag="PN977_002554"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000066594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HDI6042573.1"
                     /translation="MSNKPFHYQAPFPLKKDDTEYYLLTSEHVSVSEFEGQEILKVAP
                     EALTLLARQAFHDASFMLRPAHQQQVADILRDPEASENDKYVALQFLRNSDIAAKGIL
                     PTCQDTGTAIIVGKKGQRVWTGGGDEAALARGVYNTYIEDNLRYSQNAPLDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCIAKGGGSANKTYLYQETKALLTPGKLKNYLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAKYYDELPTEGNEHGQAFRDVELEKEL
                     LIEAQNLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINRQGIW
                     IEKLEHNPGKYIPEELRKAGEGEAVRVDLNRPMKEILAQLSQYPVSTRLSLNGTIIVG
                     RDIAHAKLKERMDNGEGLPQYIKDHPIYYAGPAKTPEGYASGSLGPTTAGRMDSYVDQ
                     LQAQGGSMIMLAKGNRSQQVTDACKKHGGFYLGSIGGPAAVLAQGSIKSLECVEYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIQLTQCTRCVK"
     gene            3470..4873
                     /gene="fumC"
                     /locus_tag="PN977_002555"
     CDS             3470..4873
                     /gene="fumC"
                     /locus_tag="PN977_002555"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001099101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HDI6042574.1"
                     /translation="MNTVRSEKDSMGAIDVPADKLWGAQTQRSLEHFRISTEKMPTSL
                     IHALALTKRAAAKVNEDLGLLSEEKASAIRQAADEVLAGQHDDEFPLAIWQTGSGTQS
                     NMNMNEVLANRASELLGGVRGMERKVHPNDDVNKSQSSNDVFPTAMHVAALLALRKQL
                     IPQLKTLTQTLNEKSRAFADIVKIGRTHLQDATPLTLGQEISGWVAMLEHNLKHIEYS
                     LPHVAELALGGTAVGTGLNTHPEYARRVADELAVITCAPFVTAPNKFEALATCDALVQ
                     AHGALKGLAASLMKIANDVRWLASGPRCGIGEIAIPENEPGSSIMPGKVNPTQCEALT
                     MLCCQVMGNDVAINMGGASGNFELNVFRPMVIHNFLQSVRLLADGMESFNKHCAVGIE
                     PNRERINQLLNESLMLVTALNTHIGYDKAAEIAKKAHKEGLTLKAAALALGYLSEAEF
                     DSWVRPEQMVGSMKAGG"
     gene            complement(4870..5799)
                     /gene="tus"
                     /locus_tag="PN977_002556"
     CDS             complement(4870..5799)
                     /gene="tus"
                     /locus_tag="PN977_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication terminus site-binding protein"
                     /protein_id="HDI6042575.1"
                     /translation="MARYDLVDRLNTTFRQMEQELAAFAAHLEQHKLLVARVFSLPEV
                     KKEDEHNPLNRIEVKQHLGNDAQSLALRHFRHLFIQQQSENRSSKAAVRLPGVLCYQV
                     DNLSQAALVSHIQHINKLKTTFEHIVTVESELPTAARFEWVHRHLPGLITLNAYRTLT
                     VLHDPATLRFGWANKHIIKNLHRDEVLAQLEKSLKSPRSVAPWTREEWQRKLEREYQD
                     IAALPQNAKLKIKRPVKVQPIARVWYKGDQKQVQHACPTPLIALINRDNGAGVPDVGE
                     LLNYDADNVQHRYKPQAQPLRLIIPRLHLYVAD"
     gene            complement(5875..7176)
                     /gene="rstB"
                     /locus_tag="PN977_002557"
     CDS             complement(5875..7176)
                     /gene="rstB"
                     /locus_tag="PN977_002557"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RstB"
                     /protein_id="HDI6042576.1"
                     /translation="MKKLFIQFYLLLFVCFLVMSLLVGLVYKFTAERAGKQSLDDLMN
                     SSLYLMRSELREIPPHDWGKTLKEMDLNLSFDLRVEPLSKYHLDDISMHRLRGGEIVA
                     LDDQYTFLQRIPRSHYVLAVGPVPYLYYLHQMRLLDIALIAFIAISLAFPVFIWMRPH
                     WQDMLKLEAAAQRFGDGHLNERIHFDEGSSFERLGVAFNQMADNINALIASKKQLIDG
                     IAHELRTPLVRLRYRLEMSDNLSAAESQALNRDISQLEALIEELLTYARLDRPQNELH
                     LSEPDLPLWLSTHLADIQAVTPDKTVRIKTLMQGHYAALDMRLMERVLDNLLNNALRY
                     CHSTVETSLLLSGNRATLIVEDDGPGIAPENREHIFEPFVRLDPSRDRSTGGCGLGLA
                     IVHSIALAMGGTVNCDTSELGGARFSFSWPLWHNIPQFTSA"
     gene            complement(7180..7899)
                     /gene="rstA"
                     /locus_tag="PN977_002558"
     CDS             complement(7180..7899)
                     /gene="rstA"
                     /locus_tag="PN977_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RstA"
                     /protein_id="HDI6042577.1"
                     /translation="MNTIVFVEDDAEVGALIAAYLAKHDMQVTVEPRGDRAEETILRE
                     NPDLVLLDIMLPGKDGMTICRDLRAKWSGPIVLLTSLDSDMNHILALEMGACDYILKT
                     TPPAVLLARLRLHLRQNEQATVTKGIQETSLTPYKALHFGTLTIDPINRVVTLANTEI
                     SLSTADFELLWELATHAGQIMDRDALLKNLRGVSYDGLDRSVDVAISRLRKKLLDNAA
                     EPYRIKTVRNKGYLFAPHAWE"
     gene            8028..8363
                     /locus_tag="PN977_002559"
     CDS             8028..8363
                     /locus_tag="PN977_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlpM family protein"
                     /protein_id="HDI6042578.1"
                     /translation="MGLVIKAALGALVVLLIGVLAKTKNYYIAGLIPLFPTFALIAHY
                     IVASERGIEALRATIIFSMWSIIPYFVYLASLWYFTGMMRLPAAFVGSVACWGISAWV
                     LIICWIKLH"
     gene            complement(8360..9082)
                     /gene="folM"
                     /locus_tag="PN977_002560"
     CDS             complement(8360..9082)
                     /gene="folM"
                     /locus_tag="PN977_002560"
                     /EC_number="1.5.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydromonapterin reductase"
                     /protein_id="HDI6042579.1"
                     /translation="MGKAQPLPILITGGGRRIGLALAWHFINQKQPVIISYRTHYPAI
                     DGLINAGAQCIQADFSTNDGVMAFADEVLKSTHGLRAILHNASAWMAEKPGTSLTDVL
                     ACMMQIHVNTPYLLNHALERLLRGHGHAASDIIHFTDYVVERGSDKHIAYAASKAALD
                     NMTRSFARKLAPEVKVNSIAPSLILFNEHDDAEYRQQALNKSLMKTAPGEKEVIDLVD
                     YLLTSCFVTGRSFPLDGGRHLR"
     gene            complement(9119..10501)
                     /locus_tag="PN977_002561"
     CDS             complement(9119..10501)
                     /locus_tag="PN977_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDI6042580.1"
                     /translation="MEKKLGLSALTALVLSSMLGAGVFSLPQNMAAVASPAALLIGWG
                     ITGAGILLLAFAMLILTRIRPELDGGIFTYAREGFGELIGFCSAWGYWLCAVIANVSY
                     LVIVFSALSFFTDTPELRLFGDGNTWQSIVGASALLWIVHFLILRGVQTAASINLVAT
                     LAKLLPLGLFVVLAMMMFKLDTFKLDFTGLALGVPVWEQVKNTMLITLWVFIGVEGAV
                     VVSARARNKRDVGKATLLAVLSALGVYLLVTLLSLGVVARPELAEIRNPSMAGLMVEM
                     MGPWGEIIIAAGLIVSVCGAYLSWTIMAAEVPFLAATHKAFPRIFARQNAQAAPSASL
                     WLTNICVQICLVLIWLTGSDYNTLLTIASEMILVPYFLVGAFLLKIATRPLHKAVGVG
                     ACIYGLWLLYASGPMHLLLSVVLYAPGLLVFLYARKTHTHDNVLNRQEMVLIGMLLIA
                     SVPATWMLVG"
     gene            complement(10687..11631)
                     /locus_tag="PN977_002562"
     CDS             complement(10687..11631)
                     /locus_tag="PN977_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HDI6042581.1"
                     /translation="MKLKNTLLASALLSATAFSVNAATELTPEQAAAVKPFDRVVVTG
                     RFNAIGEAVKAVSRRADKEGAASFYVVDTSDFGNSGNWRVVADLYKADAEKAEETSNR
                     VINGVVELPKDQAVLIEPFDTVTVQGFYRSQPEVNDAITKAAKAKGAYSFYIVRQIDA
                     NQGGNQRITAFIYKKDAKKRIVQSPDVIPADSEAGRAALAAGGEAAKKVEIPGVATTA
                     SPSSEVGRFFETQSSKGGRYTVTLPDGTKVEELNKATAAMMVPFDSIKFSGNYGNMTE
                     VSYQVAKRAAKKGAKYYHITRQWQERGNNLTVSADLYK"
     gene            12156..13688
                     /gene="pntA"
                     /locus_tag="PN977_002563"
     CDS             12156..13688
                     /gene="pntA"
                     /locus_tag="PN977_002563"
                     /EC_number="1.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HDI6042582.1"
                     /translation="MRIGIPRERLTNETRVAATPKTVEQLLKLGFTVAVESGAGQLAS
                     FDDKAFVQAGAEIVEGNSVWQSEIILKVNAPLDDEIALLNPGTTLVSFIWPAQNPELM
                     QKLAERNVTVMAMDSVPRISRAQSLDALSSMANIAGYRAIVEAAHEFGRFFTGQITAA
                     GKVPPAKVMVIGAGVAGLAAIGAANSLGAIVRAFDTRPEVKEQVQSMGAEFLELDFKE
                     EAGSGDGYAKVMSDAFIKAEMELFAAQAKEVDIIVTTALIPGKPAPKLITREMVDSMK
                     AGSVIVDLAAQNGGNCEYTVPGEIFTTENGVKVIGYTDLPGRLPTQSSQLYGTNLVNL
                     LKLLCKEKDGNITVDFDDVVIRGVTVIRAGEITWPAPPIQVSAQPQAAQKAAPEVKTE
                     EKCACSPWRKYALMALAIILFGWMASVAPKEFLGHFTVFALACVVGYYVVWNVSHALH
                     TPLMSVTNAISGIIVVGALLQIGQGGWVSFLSFIAVLIASINIFGGFTVTQRMLKMFR
                     KN"
     gene            13699..15087
                     /gene="pntB"
                     /locus_tag="PN977_002564"
     CDS             13699..15087
                     /gene="pntB"
                     /locus_tag="PN977_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000014033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit beta"
                     /protein_id="HDI6042583.1"
                     /translation="MSGGLVTAAYIVAAILFIFSLAGLSKHETSRQGNNFGIAGMAFA
                     LIATIFGPDTGNVGWILLAMVIGGAIGIRLAKKVEMTEMPELVAILHSFVGLAAVLVG
                     FNSYLHHDAGMAPVLVNIHLTEVFLGIFIGAVTFTGSVVAFGKLCGKISSKPLMLPNR
                     HKMNLAALIVSFLLLIVFVRTDSVGLQVLALLIMTAIALVFGWHLVASIGGADMPVVV
                     SMLNSYSGWAAAAAGFMLSNDLLIVTGALVGSSGAILSYIMCKAMNRSFISVIAGGFG
                     TDGSSTGDDQEVGEHREITAEETAELLKNSHSVIITPGYGMAVAQAQYPVAEITEKLR
                     ARGINVRFGIHPVAGRLPGHMNVLLAEAKVPYDIVLEMDEINDDFADTDTVLVIGAND
                     TVNPAAQDDPKSPIAGMPVLEVWKAQNVIVFKRSMNTGYAGVQNPLFFKENTHMLFGD
                     AKASVDAILKAL"
     gene            complement(15112..16146)
                     /gene="tqsA"
                     /locus_tag="PN977_002565"
     CDS             complement(15112..16146)
                     /gene="tqsA"
                     /locus_tag="PN977_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2 transporter TqsA"
                     /protein_id="HDI6042584.1"
                     /translation="MAKPIITLNGLKIVIMLGMLVIILCGIRFAAEIIVPFILALFIA
                     VILNPLVQHMVRWRVPRVLAVSILMTIIVMAMVLLLAYLGSALNELTRTLPQYRNSIM
                     TPLQAIEPLLQRVGIDVSVDQLAHYIDPNAAMTLLTNLLTQLSNAMSSIFLLLLTVLF
                     MLLEVPQLPGKFQQMMVRPVEGMAAIQRAIDSVSHYLVLKTAISIITGLVAWAMLAAL
                     DVRFAFVWGLLAFALNYIPNIGSVLAAIPPIAQVLVFNGFYEALLVLAGYLLINLVFG
                     NILEPRIMGRGLGLSTLVVFLSLIFWGWLLGPVGMLLSVPLTIIVKIALEQTTGGQSI
                     AVLLSDLNKE"
     gene            16558..16923
                     /gene="mdtJ"
                     /locus_tag="PN977_002566"
     CDS             16558..16923
                     /gene="mdtJ"
                     /locus_tag="PN977_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtJ"
                     /protein_id="HDI6042585.1"
                     /translation="MYIYWILLGLAIATEITGTLSMKWASVSEGNGGFILMLVMISLS
                     YIFLSFAVKKIALGVAYALWEGIGILFITLFSVLLFDESLSLMKIAGLTTLVAGIVLI
                     KSGTRKARKPELEVNHGAV"
     gene            16910..17239
                     /gene="mdtI"
                     /locus_tag="PN977_002567"
     CDS             16910..17239
                     /gene="mdtI"
                     /locus_tag="PN977_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtI"
                     /protein_id="HDI6042586.1"
                     /translation="MAQFEWVHAAWLALAIVLEIVANVFLKFSDGFRRKIFGLLSLAA
                     VLAAFSALSQAVKGIDLSVAYALWGGFGIAATLAAGWILFGQRLNRKGWIGLVLLLAG
                     MIMVKLA"
     gene            complement(17278..18099)
                     /locus_tag="PN977_002568"
     CDS             complement(17278..18099)
                     /locus_tag="PN977_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HDI6042587.1"
                     /translation="MRTTIAVVLGAISLTSAFVFADKPDVAKSANDEVSTLFFGHDDR
                     VPVNDTTQSPWDAVGQLETASGNLCTATLIAPNLALTAGHCLLTPPKGKADKAVALRF
                     VSNKGLWRYEIHDIEGRVDPTLGKRLKADGDGWIVPPAAAPWDFGLIVLRNPPSGITP
                     LPLFEGDKAALTAALKAAGRKVTQAGYPEDHLDTLYSHQNCEVTGWAQTSVMSHQCDT
                     LPGDSGSPLMLHTDDGWQLIGVQSSAPAAKDRWRADNRAISVTGIRDKLDQLSQK"
     gene            complement(18199..18282)
                     /locus_tag="PN977_002569"
     CDS             complement(18199..18282)
                     /locus_tag="PN977_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6042588.1"
                     /translation="MVGRYRFEFILIILILCALIAARFYLS"
     gene            complement(18375..18710)
                     /locus_tag="PN977_002570"
     CDS             complement(18375..18710)
                     /locus_tag="PN977_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid shock protein"
                     /protein_id="HDI6042589.1"
                     /translation="MKKQIEGMTMKKVLALVVAAAMGLSSAAFAAETATTPAPTATTT
                     KAAPAKTTHHKKQHKAAPAQKAQAAKKHHKNAKAEQKAPEQKAQAAKKHAKKHSHQQP
                     AKPAAQPAA"
     gene            complement(19108..20361)
                     /locus_tag="PN977_002571"
     CDS             complement(19108..20361)
                     /locus_tag="PN977_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDI6042590.1"
                     /translation="MSRTTTVDGAPASDTDKQSISQPNQFIKRGTPQFMRVTLALFSA
                     GLATFALLYCVQPILPVLSQEFGLTPANSSISLSISTAMLAIGLLFTGPLSDAIGRKP
                     VMVTALLLASICTLLSTMMTSWHGILIMRALIGLSLSGVAAVGMTYLSEEIHPSFVAF
                     SMGLYISGNSIGGMSGRLISGVFTDFFNWRIALAAIGCVALASALMFWKILPESRHFR
                     PTSLRPKTLFINFRLHWRDRGLPLLFAEGFLLMGSFVTLFNYIGYRLMLSPWHVSQAV
                     VGLLSLAYLTGTWSSPKAGTMTTRYGRGPVMLFSTGVMLFGLLMTLFSSLWLIFAGML
                     LFSAGFFAAHSVASSWIGPRAKRAKGQASSLYLFSYYLGSSIAGTLGGVFWHNYGWNG
                     VGAFIALMLVIALLVGTRLHHRLHA"
     gene            20468..21361
                     /locus_tag="PN977_002572"
     CDS             20468..21361
                     /locus_tag="PN977_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDI6042591.1"
                     /translation="MNIELRHLRYFVAVAEELHFGRAAARLNISQPPLSQQIQALEQQ
                     IGARLLARTNRSVFLTAAGKQFLADSRQILSMVDDAAARAERLHQGEAGELRIGFTSS
                     APFIRAVSDTLSLFRRDYPDVHLQTREMNTREQIAPLIEGTLDMGLLRNTALPETLEH
                     AVIVHEPLMAMIPHDHPLANNPSVTLAELAKEPFVFFDPHVGTGLYDDILGLMRRYHL
                     TPVITQEVGEAMTIIGLVSAGLGVSILPASFKRVQLNEMRWVPIAEEDAVSEMWLVWP
                     KHHEQSPAARNFRIHLLNALR"
     gene            21496..22716
                     /gene="mlc"
                     /locus_tag="PN977_002573"
                     /gene_synonym="dgsA"
     CDS             21496..22716
                     /gene="mlc"
                     /locus_tag="PN977_002573"
                     /gene_synonym="dgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor Mlc"
                     /protein_id="HDI6042592.1"
                     /translation="MVAENQPGHIDQIKQTNAGAVYRLIDQLGPVSRIDLSRLAQLAP
                     ASITKIVREMLEAHLVQELEIKEAGNRGRPAVGLVVETEAWHYLSLRISRGEIFLALR
                     DLSSKLVVEEAQELALKDDSPLLDRIISHIDQFFIRHQKKLERLTSIAITLPGIIDTE
                     NGIVHRMPFYEDVKEMPLGEALEQHTGVPVYIQHDISAWTMAEALFGASRGARDVIQV
                     VIDHNVGAGVITDGHLLHAGSSSLVEIGHTQVDPYGKRCYCGNHGCLETIASVDSILE
                     LAQLRLNQSMSSMLHGQPLTVDSLCQAALRGDLLAKDIITGVGAHVGRILAIMVNLFN
                     PQKILIGSPLSKAADILFPVISDSIRQQALPAYSQHISVESTQFSNQGTMAGAALVKD
                     AMYNGSLLIRLLQG"
     gene            22841..23536
                     /gene="bioD"
                     /locus_tag="PN977_002574"
     CDS             22841..23536
                     /gene="bioD"
                     /locus_tag="PN977_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent dethiobiotin synthetase BioD"
                     /protein_id="HDI6042593.1"
                     /translation="MLKRFFITGTDTSVGKTVVSRALLQALASQGKTVAGYKPVAKGS
                     KETPEGLRNKDALVLQSVSTIELPYEAVNPIALSEEESSVAHSCPINYTLISNGLANL
                     TDKVDHVVVEGTGGWRSLMNDLRPLSEWVVQEQLPVLMVVGIQEGCINHALLTAQAIA
                     NDGLPLIGWVANRINPGLAHYAEIIDVLGKKLPAPLIGELPYLPRAEQRELGQYIRLA
                     MLRSVLAVDRVTV"
     gene            complement(23489..24781)
                     /gene="clcB"
                     /locus_tag="PN977_002575"
     CDS             complement(23489..24781)
                     /gene="clcB"
                     /locus_tag="PN977_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated ClC-type chloride channel ClcB"
                     /protein_id="HDI6042594.1"
                     /translation="MHHLHIYPDLSTMFRRLLIATVVGILAAFAVAGFRHAMLLLEWL
                     FLNNDSGSLVNAATNLSPWRRLLTPALGGLAAGLLLMGWQKFTQQRPHAPTDYMEALQ
                     TDGQFDYAASLVKSLASLLVVTSGSAIGREGAMILLAALAASCFAQRFTPRQEWKLWI
                     ACGAAAGMAAAYRAPLAGSLFIAEVLFGTMMLASLGPVIISAVVALLVSNLINHSDAL
                     LYSVQLSVTVQARDYALIISTGVLAGLCGPLLLTLMNACHRGFVSLKLAPPWQLALGG
                     LIVGLLSLFTPAVWGNGYSTVQSFLTAPPLLMIIAGIFLCKLFAVLASSGSGAPGGVF
                     TPTLFIGLAIGMLYGRSLGLWFPDGEEITLLLGLTGMATLLAATTHAPIMSTLMICEM
                     TGEYQLLPGLLIACVIASVISRTLHRDSIYRQHTAKHS"
     gene            complement(24940..25554)
                     /gene="dmsD"
                     /locus_tag="PN977_002576"
     CDS             complement(24940..25554)
                     /gene="dmsD"
                     /locus_tag="PN977_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tat proofreading chaperone DmsD"
                     /protein_id="HDI6042595.1"
                     /translation="MTHFSQQDNFSVAARVLGALFYYAPESAEAAPLVAALTRDGWET
                     QWPLPEESLAPLVTAFQTQSEETHAQAWQRLFVGPWALPSPPWGSVWLDRESVLFGDS
                     TLALRQWMREKGIQFEMQQNEPEDHFGSLLLMAAWLAENGRQTECEELLAWHLFPWST
                     RFLDVFIEKAEHPFYCALGELARLTLAQWQSQLLIPVAVKPLFR"
     gene            complement(25597..26451)
                     /locus_tag="PN977_002577"
     CDS             complement(25597..26451)
                     /locus_tag="PN977_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit"
                     /protein_id="HDI6042596.1"
                     /translation="MGNGWHEWPLVIFTVLGQCVVGALIVSGIGWFAAKNDADRQRIV
                     RGMFFLWLLMGIGFIASVMHLGSPLRAFNSLNRIGDSGLSNEIAAGSIFFAVGGLWWL
                     VAVIGKMPQALGKLWLLVSMALGVIFVWMMTCVYQIDTVPTWHNGYTTLAFFLTVLLS
                     GPILAAAILRAARVTFNTTPFAIISVLALIACAGVIVLQGLSLASIHSSVQQASALVP
                     DYASLQVWRVVLLCAGLGCWLCPLIRRREPHVAGLVLGLILILGGEMIGRVLFYGLHM
                     TVGMAIAG"
     gene            complement(26453..27070)
                     /gene="dmsB"
                     /locus_tag="PN977_002578"
     CDS             complement(26453..27070)
                     /gene="dmsB"
                     /locus_tag="PN977_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit B"
                     /protein_id="HDI6042597.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDFKDLGPEVSFRRIYEYAG
                     GDWQEDNGVWHQNVFAYYLSISCNHCDDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNAEKGHMTKCDGCYSRVAEGKQPICVESCPLRALEFGPIEELRQ
                     KHGTLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPEEV"
     gene            complement(27081..>28199)
                     /locus_tag="PN977_002579"
     CDS             complement(27081..>28199)
                     /locus_tag="PN977_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416105.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HDI6042598.1"
                     /translation="TWTDAIDHGTEMTATRDGVRGKEKLDVPIKFLWCYASNTLINQH
                     GDINHTHEVLQDDSKCEMIVGIDHFMTASAKYCDILLPDLMPTEQEDLISHESAGNMG
                     YVILAQPATSAKFERKPIYWILSEVAKRLGPDVYQTFTEGRSQHEWIKYLHAKTKERN
                     PEMPDYEEMKTTGIFKKKCPEEHYVAFRAFREDPQANPLKTPSGKIEIYSERLATIAD
                     TWELKKDEIIHPLPAYTPGFDGWDDPLRKTYPLQLTGFHYKARTHSSYGNIDVLQQAC
                     PQEVWINPIDAQARGIRHGDTVRVFNTNGEMLIAAKVTPRILPGVTAIGQGAWLKADM
                     FGDRVDHGGSINILTSHRPSPLAKGNPSHSNLVQIEKV"
BASE COUNT         7189 a         6930 c         7314 g         6766 t
ORIGIN      
        1 agctgactgc tgaatacgcc gcgatgatag ttttgataac tcacttccag gttggattca
       61 ggagcggtca gtttgagttg cgcgttcgcc tgcgcgacca tgtcttcgag atgggtttca
      121 atcttcttgc ctgtatacca tgcgccgcct gtccagacta cgcctagcgc aacaatgacg
      181 cctaccgcta ccagcgattt attcataatg attatccata aaatgaaatc aggcggactg
      241 gccgcctgaa ggtgttataa gcctttaata agcttagcaa gagatgttaa ttttttcagt
      301 aagctcttac agcttgttgt aaacacgcgc taaacgaccg tggcctttga cagtcaccgg
      361 tgattcgttg gcggcaataa acgctgattc acccggttta agctgtaact gctgagaacc
      421 tttacacagc gttgcatcgc cttcgacgca gaacaaaatg gcggcactct gctggctaat
      481 ggtggtttct ttatcactaa ggtcatgcag cgagaaggca aaatcatcca caggaatcgg
      541 gaagtccagt tctgcacctt gtttcaccgg ctgggtcaac aactggttag ccggtttggc
      601 ttcgaacttc acattggcaa ccagttccgg aatatcaata tatttaggcg tcagacccgc
      661 acgcagcacg ttatcggagt tcgccatcac ttccagcgcc acgccttgca ggtaagcgtg
      721 cggcgtttca gcgaacagga acatcgcttc gccaggattc aatttcacca cattcagcaa
      781 cagcggggag aacagaccgc tgtcatccgg gtaaaattca gaaattaaac gaatcgtttg
      841 ccacggttca ccctgctggc tgtcgagggc cgattttaaa atcgccagcg cgcgggattt
      901 ttcttcaccc tgcatattca acaggctggc gaacagttcg cttaaacgtt cggcatcagg
      961 ctgttgtaaa aagtgagcaa tcgccggatg tgcacctgcg accggctgga gcagggagac
     1021 aatctcggaa aactcacgaa acgcgttcat cgcaaggaaa ggcgtcagcg caaaaaccag
     1081 ctccggcttg tggttaggat ctttatagtt acgctcggcg gcatccatcg gaatacctgc
     1141 ggcattttct ttggcaaaac cgatttcaga attgcgtttg tttggatgaa cctgaatgga
     1201 gagcggctgt gctgcgcata atactttgaa caggaaaggc agttcgccaa agcgtttggc
     1261 aacggcatct cccaaaagag tcgctttatc actctcaatc acatcacgca gtgaaacgat
     1321 atctccggcg gcattctgca ctcgtgaact gcttttcgga tgtgcgccca tccacagctc
     1381 ggccatcggc tggctggacg gattttccat accataaagt tcagtcaacg ccgttttgct
     1441 gccccaggca tagttttgca ctgagttaat gagtttttgc atgatcaatc cctgttttaa
     1501 tgtggaaatt aatcccgcta ttaaagcaag aatcctacgg gaagtaacct ggagccgcaa
     1561 aaagtcgtac tagtctcagt ttttgttaaa aaagtgtgta ggatattgtt actcgctttt
     1621 aacagggcaa cggaacaccc gcccagagca taaccaaacc aggcagtaag tgagagaaca
     1681 atgtcaaaca aaccctttca ttatcaggct ccttttccac tcaaaaaaga tgatactgag
     1741 tattacctgc taaccagcga acacgttagc gtatctgaat ttgaagggca ggagattttg
     1801 aaagtcgcac ccgaagcgtt aactctgttg gcgcgtcaag cgtttcatga tgcgtcattt
     1861 atgctgcgtc cggctcacca acaacaggtg gccgacattc tgcgtgaccc ggaagccagc
     1921 gaaaatgata aatatgtggc gctgcaattc ctgcgtaact ccgacatcgc ggcgaaaggc
     1981 attctgccga cctgtcagga taccggcacc gcgattattg ttggtaaaaa agggcagcgt
     2041 gtatggaccg gtggcggtga tgaagcggcg ctggcacgcg gtgtctataa cacttatatc
     2101 gaagataatc tgcgctactc gcaaaacgcg ccgctggata tgtataaaga ggtgaatacc
     2161 ggcaccaatc taccagcgca gatcgatctt tatgccgttg atggcgacga gtacaaattc
     2221 ctctgtatcg ccaaaggtgg cggttcggca aacaagacgt atctgtatca ggaaaccaaa
     2281 gcgttactaa cgccggggaa actgaaaaat tacctggttg agaagatgcg cacgctgggt
     2341 acggcggcct gtcctccgta tcatattgcg ttcgttattg gtggaacttc tgcagaaacg
     2401 aaccttaaaa cggtgaaact ggcttccgct aaatactatg atgaactgcc aacggaaggg
     2461 aatgagcacg gacaggcgtt ccgcgatgtg gaactggaaa aagaattgct gatcgaagcg
     2521 caaaatcttg gtctgggtgc gcagtttggt ggtaaatact tcgctcacga catccgcgtg
     2581 attcgcctgc cacgtcacgg cgcatcctgc ccggtcggta tgggcgtctc ctgttctgct
     2641 gaccgtaata tcaaagcgaa gatcaaccgt caggggatct ggatcgaaaa actggaacat
     2701 aatccaggaa aatatatccc ggaagagctg cgcaaagcgg gagaaggcga agcggtgcgc
     2761 gttgacctta accgtccgat gaaagagatc ctcgcacagt tgtcgcagta tcccgtttct
     2821 acacgcttat cgctgaatgg cacgattatc gtcggtcgtg atattgctca cgccaaactg
     2881 aaagagcgga tggataacgg tgaagggctg ccgcagtaca tcaaagatca tccgatttac
     2941 tatgcgggtc cggccaaaac gccggaaggt tatgcctccg gttctcttgg cccaacgacc
     3001 gccggacgga tggattctta tgtcgatcaa ctgcaagcgc agggaggaag tatgatcatg
     3061 ctggcgaaag gcaaccgcag ccagcaggtg acggatgcct gtaaaaaaca cggcggtttc
     3121 taccttggca gtatcggtgg tccggccgct gtattggcgc agggtagtat taagagcctg
     3181 gaatgtgttg aatatccgga actgggaatg gaagccatct ggaaaattga agtggaagat
     3241 ttcccggcat ttatccttgt ggatgataaa ggaaatgact tcttccagca gatacaactc
     3301 acacagtgca cccgctgtgt gaaataaaca gagccgccct tcggggcggt ttttttacat
     3361 ggcacgaaag accaaacatt tgttatcaaa tagtaaataa tgagtgagct aaaagttgct
     3421 taacgaaagc aaaacagaaa gaaaaaatta atcaggtgag gagcaggcca tgaatacagt
     3481 acgcagcgaa aaagattcga tgggggcgat tgatgtcccg gcagataagc tgtggggcgc
     3541 acaaactcaa cgctcgctgg agcatttccg catttcgacg gagaaaatgc ccacctcact
     3601 gattcatgcg ctggcactaa ccaagcgcgc agcggcaaaa gttaatgaag atttaggctt
     3661 gttgtctgaa gagaaagcga gcgccattcg tcaggcggcg gatgaagtac tggcaggaca
     3721 gcatgacgac gaattcccgc tggctatctg gcagaccggc tccggcacgc aaagtaatat
     3781 gaacatgaac gaagtgctgg ctaatcgggc cagtgaatta cttggcggcg tgcgcgggat
     3841 ggagcgtaaa gttcacccta acgacgacgt gaacaaaagc caaagttcca atgatgtctt
     3901 tccgacggcg atgcacgttg cggcgctact ggcgctgcgc aagcaactca ttccgcagct
     3961 taaaaccctg acacagacac tgaatgagaa atcccgtgca tttgccgata tcgtcaaaat
     4021 cggtcgaacc cacttgcagg atgccacgcc gctaacactg gggcaggaga tttccggctg
     4081 ggtagcgatg ctcgagcata atctcaaaca tatcgaatac agcctgccgc atgtagcgga
     4141 actggctctt ggcggtacag cggtgggtac tggactaaat acccatccgg agtatgcgcg
     4201 tcgagtagca gatgaactgg cagtcattac ctgcgctccg tttgttaccg cgccgaacaa
     4261 atttgaagcg ctggcgacct gtgatgccct ggttcaggcg cacggcgcgt tgaaagggtt
     4321 ggctgcgtca ctgatgaaaa ttgccaatga tgtccgctgg ctggcctctg gcccgcgctg
     4381 tggaattggt gaaatcgcaa tcccggaaaa tgagccgggc agctcaatca tgccggggaa
     4441 agtgaaccca acccagtgtg aggcattaac catgctttgc tgtcaggtga tggggaacga
     4501 cgtggcgatc aacatgggtg gcgcttccgg taactttgaa ctgaacgtct tccgtccgat
     4561 ggtgattcat aatttcctgc aatcggtgcg cttgctggca gatggcatgg aaagtttcaa
     4621 caaacactgt gcagtgggca ttgaaccgaa tcgtgagcga atcaatcaat tactcaatga
     4681 atcgctgatg ctggtgactg cgcttaacac ccacattggt tatgacaaag ccgccgagat
     4741 cgccaaaaaa gcgcataaag aagggctgac cttaaaagct gcggcccttg cgctggggta
     4801 tcttagcgaa gccgagtttg acagctgggt acggccagaa cagatggtcg gcagtatgaa
     4861 agccgggggt taatctgcaa catacaggtg cagccgtgga atgatcaaac gaagcggctg
     4921 cgcctgtggt ttataacggt gctgcacatt gtcggcatcg taatttaaca actcaccaac
     4981 gtccggcacg cccgcgccat tatcccgatt aatcagtgca atcagcggcg tagggcaagc
     5041 gtgttgtact tgtttttgat ctcctttgta ccagacgcgg gcaatcggct gcactttcac
     5101 cggacgtttg atttttaact tcgcgttctg tggcagggca gcgatatcct gatattctcg
     5161 ctccagtttt ctttgccact cctcgcgcgt ccacggtgcg acactgcgtg gtgatttcag
     5221 gcttttttcc agctgtgcca ggacttcatc acgatgcaaa tttttaatga tatgtttatt
     5281 agcccagcca aaacgtaaag tggcggggtc gtgcagaaca gtgagcgtgc ggtaggcatt
     5341 aagggtgatc agccccggca aatgacgatg cacccattca aaacgtgccg cggtggggag
     5401 ttctgattca accgtgacaa tatgctcgaa cgtggtcttg agtttattga tgtgctgaat
     5461 atgactgacc aacgctgctt gcgaaaggtt atcgacctgg taacacaaca cgccaggtag
     5521 acgaacagcg gccttgctgc tgcgattttc ggactgttgt tgaataaata aatggcggaa
     5581 atgacgcaac gccagcgact gcgcgtcgtt gccgagatgt tgttttacct caatacgatt
     5641 aagcggatta tgctcatcct ctttttttac ctccggcaaa gagaacacgc gggcaaccaa
     5701 tagcttgtgt tgctcaagat gagcggcaaa tgcagccagc tcttgttcca tctggcgaaa
     5761 agtagtgttg agtcggtcta cgagatcgta acgcgccata atattaacca ctttagttac
     5821 aacatcctta ttttatagca caatggtggt gacttgacca ccgtgcgcat agtgtcaggc
     5881 agaggtaaat tgcgggatgt tatgccataa cggccagcta aacgagaagc gggcaccacc
     5941 cagttcgctg gtgtcacaat taaccgtacc gcccattgcc agtgctatag agtggacaat
     6001 tgccagcccc agcccgcagc cgccggttga gcgatcccgg ctgggatcga ggcgaacaaa
     6061 aggttcaaag atatgttcgc ggttttctgg ggcaatccct gggccatcat cctcaacaat
     6121 taatgtcgct ctattccccg acagtagcag gctggtttca accgttgaat gacagtagcg
     6181 cagggcgtta ttgagcaaat tatccagcac gcgctccatt aagcgcatat ccaacgccgc
     6241 ataatggcct tgcatgagcg tttttatccg taccgtttta tcgggcgtta ctgcctgaat
     6301 atctgccaga tgcgttgaca gccacaacgg caggtctggt tcgctaagat gaagctcatt
     6361 ttgtgggcga tcgagtcggg cataagtcag aagctcttca attaaagctt caagttgact
     6421 gatatcgcgg ttcaacgcct gggattcggc ggcgctcagg ttatcgctca tctccagtcg
     6481 ataacgcagg cgcactaacg gtgttcgcag ttcgtgagcg ataccgtcaa taagctgttt
     6541 tttgctggca atcaaggcgt tgatattgtc cgccatctgg ttaaatgcaa caccaagtcg
     6601 ttcaaagctc gaaccctcat caaagtggat acgttcattg agatgcccat cgccaaatcg
     6661 ttgcgccgct gcttccagtt ttaacatatc ctgccagtgc ggacgcatcc agataaacac
     6721 cggaaaggcg agggaaatag caataaaagc gatcagggcg atatccagta atcgcatctg
     6781 atggaggtaa taaagataag gaacaggacc aactgccagc acgtagtggc tgcgcgggat
     6841 acgttgcaaa aaggtgtact gatcgtccag agcgacaatt tcgccgccac gcagccggtg
     6901 catggaaata tcatcaagat ggtatttact cagcggctcg acacgcagat cgaaagagag
     6961 atttaaatcc atctctttca gcgtcttacc ccagtcgtgt ggggggatct cacgcaattc
     7021 gctgcgcatc agatacagcg aactgttcat caaatcatcc agcgactgtt tgcctgcgcg
     7081 ttcggcggta aatttgtaca ccagcccgac cagcagagac atcacaagga agcagacaaa
     7141 caataacagg taaaactgga taaacagttt tttcatcgct tattcccacg catgaggcgc
     7201 aaaaagatag cctttgttac gcacggtttt aatacgataa ggttctgcgg cgttatcgag
     7261 cagttttttt cttaaccgcg aaatagccac gtccacgcta cgatccagac cgtcataact
     7321 gacgccgcgt aaatttttca gcaatgcatc gcggtccatg atttgcccgg catgggtagc
     7381 taattcccac aataattcga aatcagctgt cgagagcgag atttcagtgt tagccagggt
     7441 gactacgcgg ttgatgggat cgatggtcaa cgtgccgaaa tgcagggctt tgtagggagt
     7501 cagagacgtt tcctgaatac ctttggtcac tgtggcttgc tcattctgac gcaaatgcaa
     7561 acgtaaacgc gctagcaaaa cagcaggggg cgtcgttttg agaatatagt cgcaggcgcc
     7621 catttccagt gccaggatgt ggttcatatc gctatcgaga gaggttagaa gaacaatcgg
     7681 tccggaccac tttgcgcgta aatcacgaca aatggtcatg ccgtccttgc ctggtagcat
     7741 gatgtcgagt aacaccaaat ccggattttc tcgcagaata gtttcttcgg cccggtcgcc
     7801 gcgcggctct acggtaacct gcatatcatg ttttgccagg tatgcggcaa tcagtgcacc
     7861 gacttccgca tcatcttcca caaatacgat agtgttcata acattcacgg tagatataaa
     7921 aacgtcacaa tacaccgcac tgtttttact tactatcaat cgtttctaaa caaggtttca
     7981 tcaggtattc tactgatgac ttgttatgga agtgttaagg taaaaagatg gggctggtaa
     8041 tcaaagccgc gctcggtgcg ctggtagtgc tgttgattgg tgttttagca aaaacgaaaa
     8101 attattatat cgccgggctg attccacttt tcccgacctt tgcgcttatc gcgcattata
     8161 ttgttgccag cgaacgcggc attgaagcct tacgcgcaac catcattttt agtatgtggt
     8221 cgattattcc ctattttgtt tacctggcgt cgctgtggta tttcaccgga atgatgcgct
     8281 tgcccgccgc ttttgttggt tctgttgcgt gttgggggat aagcgcatgg gtgttgatta
     8341 tatgctggat aaagctgcat taacgcagat gacgaccgcc atcaagaggg aaactgcgtc
     8401 cggtgacaaa gcagctggta agtaagtaat cgaccaggtc gatcacttct ttctcgccag
     8461 gcgcggtttt catcagtgat ttattcagcg cctgttgtcg atattcggca tcatcatgtt
     8521 cattaaacag gattagcgat ggcgcaatag aattcacttt cacttccggt gccagcttgc
     8581 gggcaaacga gcgggtcata ttatccagtg ccgctttgct tgcagcatag gcaatatgtt
     8641 tgtcgctacc gcgctccacc acataatcgg taaagtgaat gatatcgctg gcggcgtgtc
     8701 cgtgcccacg cagtaatctt tccagtgcat ggttgagtag gtatggggta ttaacgtgga
     8761 tctgcatcat gcaagccagt acgtcggtca gtgatgtacc tggtttttct gccatccacg
     8821 cgctggcgtt atgcaaaata gcacgcaggc catgggtgct ttttagtact tcatcggcaa
     8881 acgccatcac accgtcgttg gtcgaaaaat cagcctgaat acactgcgca cctgcgttaa
     8941 taagtccatc aatggcggga tagtgcgtcc gatagctgat aatcaccggt tgcttttgat
     9001 taatgaaatg ccatgcgagg gcgaggccga tgcgacgacc accgccagta attaatattg
     9061 gcaagggttg ggctttaccc atcgttatct cctttgctat ccaacgatgg gatgaacgtt
     9121 atcccaccag catccaggtc gccggaactg aggcaattaa cagcatgcca atcagcacca
     9181 tttcctgacg attcagtaca ttatcatggg tatgcgtttt acgcgcgtac aggaaaacca
     9241 gcagccccgg tgcatacaga acaacggata acagcaagtg cattggacca gaggcgtaca
     9301 ataaccataa gccataaatg caggcaccga cacctaccgc tttatgcagt ggacgggttg
     9361 cgatttttag caagaacgcg ccgaccagga aatagggcac cagaatcatt tctgaggcga
     9421 tggtcagcaa tgtgttgtaa tcggaaccgg tcagccagat gagcaccaga caaatttgca
     9481 cgcagatgtt ggtcaaccac agtgaggcgg atggcgcagc ttgtgcgttc tggcgcgcaa
     9541 aaatgcgcgg gaatgcttta tgagtggcgg ccaggaacgg aacttctgcc gccatgatgg
     9601 tccagcttaa gtacgcgccg caaacggaaa cgatcaaacc ggctgcgatg atgatttcgc
     9661 cccatggtcc catcatttcc accatcagac cggccattga cgggttacga atttcagcca
     9721 gttcaggacg tgccaccaca cccagcgaaa gcagcgttac cagcaagtaa acgcccagag
     9781 cggagagaac cgccagcagt gtcgctttgc caacatcacg tttattacgc gcacgcgcag
     9841 aaacaaccac ggcaccttcc acaccaatga aaacccacag ggtgatcagc atggtgtttt
     9901 tcacctgttc ccaaacgggt acgccaagtg caagtccggt gaagtcgagc ttgaaggtat
     9961 ccagtttgaa catcatcatc gccagcacaa caaacagacc cagcggcaac agttttgcca
    10021 gtgttgccac caggttaatg ctggcagcgg tttgcacccc gcgcaggatc aaaaagtgaa
    10081 caatccacaa taaagccgat gcaccgacta tcgattgcca ggtattacca tcgccaaaca
    10141 ggcgcaattc cggcgtgtcc gtaaaaaagc ttaacgcgga aaaaacgatc accagatagg
    10201 agacgttggc gatgactgcg cacagccagt atccccatgc ggaacaaaag ccgattagct
    10261 cgccaaaccc ttcgcgggca taggtaaaga taccgccgtc aagttcggga cgaatgcgcg
    10321 tgaggatcag catggcaaag gccagcaata aaatgccagc gccagtaata ccccagccga
    10381 tgagcagtgc tgccgggctg gcaactgccg ccatattttg cggcagactg aaaacacccg
    10441 cgcccagcat tgagcttaat accagcgcgg tgagtgcgct cagtcccagt ttcttttcca
    10501 tcgatatcca gtgattaaaa cgtgataaga agaataaata ttagggcaaa acgcataaaa
    10561 atggagtttg ccactgaagc gcgggatttt acgaagagat gtgtctgcat gcaatggtcg
    10621 cggggaaatt tgtgactaaa aatgcgaaag gcggcatcaa gccgccttat aggagtaact
    10681 gaatagttat ttatacagat ctgcgctgac ggtcaggtta ttaccacgtt cctgccactg
    10741 gcgggtgatg tggtaatact tagcaccttt cttcgcggca cgtttcgcaa cctgatagga
    10801 gacttcggtc atgttgccgt agttgccaga gaatttgatg ctgtcgaacg ggaccatcat
    10861 cgctgcggtc gctttgttca gttcttcgac tttagtgcca tccgggagcg tgacggtgta
    10921 acgcccgcct tttgatgact gggtttcaaa gaagcgaccg acttcagaac ttggtgatgc
    10981 ggtagtcgca acccccggga tctcaacttt cttcgcagct tcgccaccgg cagccagagc
    11041 tgcacgtcct gcttcggaat ctgccgggat cacatccggg ctctggacga tacgtttctt
    11101 agcatctttt ttatagatga atgcagtaat acgctggttg ccgccctggt tggcatcgat
    11161 ttgacgaacg atgtagaaag agtaggcacc tttcgctttt gctgctttgg tgatggcatc
    11221 attgacttct ggctggctac gatagaagcc ctggacggtg accgtgtcaa acggttcaat
    11281 cagaacagcc tgatctttcg gcagttcgac aacaccgtta attacgcgat tacttgtttc
    11341 ttctgctttt tcagcatcgg ctttatagag gtcagcgacc acacgccagt taccgctgtt
    11401 accaaaatca gaagtgtcga caacataaaa agaggcggca ccttctttat ctgcgcgacg
    11461 agaaacggct ttcaccgctt cgccaatagc attaaaacga ccggtaacca ctacacggtc
    11521 aaaaggttta accgctgccg cttgctccgg tgtcagttct gttgctgcgt taacggagaa
    11581 tgccgtagca gaaagcagtg ccgacgccag gagggtgttc ttaagcttca taaaaataat
    11641 ccttcgcctt gcgcaaacca agtactggta ttgttattaa cgagaaacgt ggctgattat
    11701 tgcatttaaa cggtgtaact gtctgcgtca tttttcatat cacattcctt aagccaattt
    11761 taatcctgct caaatgaccg tctatgctta aaaaaacagc cgtatcagca tcaatactac
    11821 tgaatcaact gaattgtata agttaattta atgttaagta gtgattcgta ccggggcgat
    11881 gtctcgtttt acccgaccgt cgaagacaat tatcagtctt tatccagcgt tctaaggtgt
    11941 ttatcccact atcacggctg aatcgttaat attttgcgag ttcacgccga aatactgatt
    12001 tttggcgcta gatcacaggc ataattttca gtacgttata gggcgtttgt tactaattta
    12061 ttttaacgga gtaacattta gctcgtacat gagcggcttg tgtggctcct gacacaggca
    12121 aaccatcatc aataaaaccg atggaaggga atatcatgcg aattggcata ccaagagaac
    12181 ggttaaccaa tgaaacccgt gttgcagcaa cgccaaaaac agtggaacag ctgctgaaac
    12241 tgggttttac cgtcgcggta gagagcggcg cgggtcaact ggcaagtttt gacgataaag
    12301 cgtttgtgca agcgggcgct gaaattgtag aagggaatag cgtctggcag tcagagatca
    12361 ttctgaaggt caatgcgccg ttagatgatg aaattgcgtt actgaatcct gggacaacgc
    12421 tggtgagttt tatctggcct gcgcagaatc cggaattaat gcaaaaactt gcggaacgta
    12481 acgtgaccgt gatggcgatg gactctgtgc cgcgtatctc acgcgcacaa tcgctggacg
    12541 cactcagttc gatggcgaac atcgctggtt atcgcgccat tgttgaagcg gcacatgaat
    12601 ttgggcgctt ctttaccgga caaatcactg cggccgggaa agtgccaccg gcaaaagtga
    12661 tggtgattgg tgccggtgtc gcaggtctgg ccgccattgg cgcagcaaac agtctcggcg
    12721 cgattgtgcg tgcattcgac acccgcccgg aagtgaaaga acaagttcaa agtatgggcg
    12781 cagaattcct cgagctggat tttaaagagg aagcgggcag cggcgatggc tatgccaaag
    12841 tgatgtcgga cgcgttcatc aaagcggaaa tggaactctt tgccgcccag gcaaaagagg
    12901 tcgatatcat tgtcaccacc gcgcttattc caggcaaacc agcgccgaag ctaattaccc
    12961 gtgaaatggt tgactccatg aaggcgggca gtgtgattgt cgacctggca gcccaaaacg
    13021 gcggcaactg tgaatacacc gtgccgggtg aaatcttcac tacggaaaat ggtgtcaaag
    13081 tgattggtta taccgatctt ccgggccgtc tgccgacgca atcctcacag ctttacggta
    13141 ctaacctcgt taatctgctg aaactgttgt gcaaagagaa agacggcaac atcactgtgg
    13201 attttgatga tgtggtgatt cgtggcgtga ccgtgatccg tgcgggcgaa attacctggc
    13261 cggcaccgcc gattcaggta tcagctcagc cgcaggcggc acaaaaagcg gcaccggaag
    13321 tgaaaactga ggaaaaatgt gcctgctcac cgtggcgtaa atacgcgttg atggcgttgg
    13381 caatcatcct tttcggctgg atggcaagcg ttgcgccaaa agagttcctt ggacacttca
    13441 ctgtgttcgc gctggcctgc gttgtcggtt attacgtggt gtggaatgta tcgcacgcgc
    13501 tgcatacacc gttgatgtcg gtcaccaacg cgatttcagg gattattgtt gtcggagcac
    13561 tgttgcagat tggccagggc ggctgggtta gcttccttag ttttatcgcg gtgcttatag
    13621 ccagcattaa tattttcggt ggcttcaccg tgactcagcg catgctgaaa atgttccgca
    13681 aaaattaagg ggtaacatat gtctggagga ttagttacag ctgcatacat tgttgccgcg
    13741 atcctgttta tcttcagtct ggccgggctt tcgaaacatg aaacgtctcg ccagggtaac
    13801 aatttcggta tcgccggtat ggcgtttgcg ttaatcgcaa ccatttttgg accggatacg
    13861 ggtaatgttg gctggatctt gctggcgatg gttattggtg gggcaattgg tatccgtctg
    13921 gcgaagaaag ttgaaatgac cgaaatgcca gaactggtgg cgatcctgca tagcttcgtg
    13981 ggtctggcgg cagtgctggt tggttttaac agctatctgc atcatgacgc gggaatggca
    14041 ccggttctgg tcaatattca cctgacggaa gtgttcctcg gtatcttcat cggggcggta
    14101 acgttcacgg ggtcggtggt ggcgttcggc aaactgtgtg gcaagatttc gtctaaaccg
    14161 ttgatgctgc caaaccgtca caaaatgaac ctggcggctc tgatcgtttc cttcctgctg
    14221 ctgattgtat ttgttcgcac ggacagcgtc ggcctgcaag tgctggcatt gctgattatg
    14281 accgccattg cgctggtatt tggctggcat ttagtcgcct ctattggtgg tgcagatatg
    14341 ccagtggtgg tatcaatgct gaactcgtac tccggctggg cggctgcggc tgcgggcttt
    14401 atgctcagca acgacctgct gattgtgacc ggtgcgctgg tcggttcttc gggggctatc
    14461 ctttcttaca ttatgtgtaa ggcgatgaac cgttctttta tcagcgttat tgcgggtggt
    14521 ttcggcaccg acggctcttc tactggcgat gatcaggaag tgggcgagca ccgcgaaatc
    14581 actgcagaag agacagcgga actgctgaaa aactcccatt cagtgatcat tactccgggg
    14641 tacggcatgg cagtcgcgca ggcgcaatat cctgtcgctg aaattactga gaaattgcgc
    14701 gctcgtggta ttaatgtgcg tttcggtatc cacccggttg cggggcgttt gcctggacat
    14761 atgaacgtat tgctggctga agcaaaagta ccgtatgaca tcgtgctgga aatggacgag
    14821 atcaacgatg actttgctga taccgatacc gtactggtga ttggtgctaa cgatacggtt
    14881 aacccggcgg cgcaggatga tccgaagagt ccgattgctg gtatgcctgt tctggaagtg
    14941 tggaaagcgc agaacgtgat tgtctttaaa cgttcgatga acactggcta tgctggtgtg
    15001 caaaacccgc tgttcttcaa ggaaaacacc cacatgctgt ttggtgacgc caaagccagc
    15061 gtggatgcaa tcctgaaagc tctgtaaccc tgacggcctc tgcggaggcc gtcactcttt
    15121 gttgagatcg cttaacagaa cggcgatgct ttgacctccc gttgtttgtt caagcgcaat
    15181 tttgacaata attgttaacg gcacggaaag cagcataccc accggtccta acaaccatcc
    15241 ccaaaaaatc aacgacaaaa ataccaccaa tgtggaaagc cccagcccac gtcccatgat
    15301 gcgcggctcc agaatattgc cgaagaccag attaattagc agatatcccg ccagcaccag
    15361 caacgcttcg tagaagccat taaacaccag tacctgagcg atagggggga ttgccgcgag
    15421 aactgaacca atattaggga tgtaattaag cgcaaaggcc agcaatcccc agacaaaagc
    15481 gaagcgaaca tcgagtgcgg cgagcatcgc ccaggcgacc aggccggtga tgatgctgat
    15541 ggctgttttc agcaccagat aatgagacac actgtcaatc gcacgctgaa tcgccgccat
    15601 cccttcaacc ggacgcacca tcatttgctg aaattttccg ggcaattgtg gcacttcgag
    15661 caacataaac agcaccgtca gcagcaataa aaagattgat gacatggcat tagataactg
    15721 cgtcaataag ttggtgagca acgtcattgc cgcgttcgga tcaatgtaat gcgccagttg
    15781 gtcaactgag acatcaatcc ctacgcgttg caacaacggt tcaatcgctt gcagcggcgt
    15841 cataatagag ttgcgatatt gcggtaacgt ccgcgtcaac tcgttgagcg cggaacccag
    15901 ataagctaac agcaacacca tcgccatcac gatgatggtc atcaaaatcg acaccgccag
    15961 tacacgcggc actcgccagc ggaccatgtg ttgcaccagc gggttaagaa taacagcaat
    16021 aaataatgcg agaataaacg gcacgatgat ctcggcggca aaacggatac cgcagagaat
    16081 aatgaccagc attcccaaca taatgacgat ttttaggcca ttgagcgtga tgatcggctt
    16141 tgccatgctt gtccccgttt tttcttcctt tgctaataat aatgtctttt tcgctccgtc
    16201 atcataacga aaatcaaacc gctcaggtaa agaagtgaaa atattttgag ttaattctta
    16261 agctatgata caaatcaggc gtgttcaact accgaggaca attatcatcc gcgatgacga
    16321 gaagcaacac tgcggataat tgtaatatta tggacaattt gttcaggacg ttattttcta
    16381 catttaccca tcttcgtacc tcatccacta tcttgctcgt aggtgagcag cactggcgca
    16441 acgcgcttta gtcctttctc ctgcttaagc ttgcgctatg cgcggggctt gaccacttta
    16501 ctttggtttc gctgaattaa gcgaaaatta aaataattct cttgcaggag aaggacaatg
    16561 tatatttatt ggattttatt aggtctggct attgctacag aaattaccgg tacgctgtct
    16621 atgaaatggg cgagcgtcag tgagggaaat ggcggcttta ttttaatgct ggtgatgatt
    16681 tctctgtcgt atatatttct ctctttcgcc gttaaaaaga tcgccttagg cgtggcttat
    16741 gcgctgtggg aaggtatcgg tattttattt attaccttgt ttagcgtttt gttattcgac
    16801 gaaagtttat cgctgatgaa aattgccggg ttaaccaccc tggtcgccgg gattgtgttg
    16861 atcaaatcag gtactcgtaa agcgcgtaaa cctgaactgg aggtgaacca tggcgcagtt
    16921 tgaatgggtt cacgccgcct ggctggcatt ggcaatcgtg ctggaaatcg ttgctaacgt
    16981 ctttttgaaa ttttctgacg gctttcgtcg caaaatattt ggcttgctct ccctggcggc
    17041 ggtgctggct gcctttagtg cgctttctca ggccgttaaa gggatcgact tgtctgtcgc
    17101 ttatgcattg tggggcgggt ttggtattgc cgccacgtta gccgcaggtt ggatcttgtt
    17161 tggtcaacgg ttaaatcgta aaggctggat tggcctggtc ttgctgttgg ctggaatgat
    17221 catggtgaaa cttgcctgat gaacacgctg cccgcactgt cgggcagcgt ttgaacatta
    17281 tttttgcgac agttgatcca gcttgtcgcg aataccggta acagaaatgg cccggttatc
    17341 ggcgcgccag cgatctttcg cggcaggagc cgagctttgt accccaatta attgccagcc
    17401 gtcatcggta tgcaacatca gaggcgaacc gctgtctccc ggcaaggtat cgcactgatg
    17461 cgacatcacc gacgtttgcg cccagccagt cacttcacag ttttgatgac tgtacaacgt
    17521 atcgagatga tcttcagggt agcccgcctg agtcacttta cgaccagctg cttttaatgc
    17581 ggcggtaagc gcggctttat ctccctcaaa taacggcaac ggtgtaatgc cagaaggggg
    17641 attacgtagc acaatcaaac cgaagtccca cggcgcagct gcgggaggta caatccagcc
    17701 atccccatct gcttttaacc gctttcctag tgtcgggtca acgcggcctt ctatgtcgtg
    17761 gatctcatag cgccaaagac ctttatttga cacgaaacgc agcgctaccg ctttatcggc
    17821 tttacccttt ggcggcgtca ataaacagtg tcctgccgtt aatgccagat tgggtgcaat
    17881 cagcgtagcc gtacataaat tgccgctggc cgtttccagt tgcccaaccg catcccacgg
    17941 tgattgggtc gtgtcattta ctggcacacg atcatcatga ccaaaaaaca gggtgctgac
    18001 ctcatcgttt gccgatttgg caacgtctgg tttatctgca aacacaaaag cagacgtcaa
    18061 actaattgca cccaacacta cagcaatggt tgtacgcata tcacactctg gtgggtaatt
    18121 atgattatta aaagcaaacc ctcataaata ctatagacgg gacgggatga aagtgggagt
    18181 aaaatcagat aactacgatc aggaaagata aaaacgggcg gcaatcagtg cgcataaaat
    18241 aaggatgata agaatgaact caaagcgata acggcccacc atactaccct ccgctaaagg
    18301 cggtgctaaa cgttaaccgc ttagcaccgt aattttcagg cgcgaggggg cgcgccagca
    18361 ttactgttga aaacttacgc tgcgggttgt gcagcaggtt ttgccggttg ctgatggctg
    18421 tgtttcttgg cgtgtttctt cgctgcctgc gctttttgtt caggagcttt ctgttcagct
    18481 ttcgcatttt tatgatgctt tttagccgcc tgcgctttct gggcaggtgc tgctttatgc
    18541 tgttttttat gatgtgtagt tttcgccggc gctgctttgg tggtcgtcgc agtcggagca
    18601 ggtgtggtcg cagtttctgc agcaaaggcg gcagaagaca gacccatagc agcggcaaca
    18661 accagagcta atactttttt cattgtcata ccctcaattt gttttttcat ttaaccccac
    18721 tgcggggccg ttgaaataac tatatccctg ggaatggcag acttccgtga gtggttggtt
    18781 tcagcgtgta acgatatgta caaacgctga ataaattaca gcgttgataa taacgtattg
    18841 tgacttaagg gaaatttagc taccaataat agtagtcttg atcgggttaa ctattttact
    18901 acccacaaag cggtagaata actgcgcatc agtaataaat gacacacagc aaaatgaatc
    18961 cgtttatttg ggtacttata atcctgatga cgctagatgc gctgcgggaa ttggctggcg
    19021 cttcgtctat tttaggatgg ctattaacgt tggtttgagc aggcaataag tccggacggg
    19081 tatttacctc agtccggact tattttttca ggcgtgcaga cgatgatgca aacgcgtccc
    19141 gaccagcaga gcaatgacca gcatcagagc aataaatgcg ccgacgccgt tccagccata
    19201 gttatgccag aaaacaccac ccagcgtccc ggcaatactc gaccccagat agtaactgaa
    19261 cagatacagc gaggaggcct ggcctttagc gcgttttgcg cgggggccga tccagctgct
    19321 ggcaacggag tgggctgcga agaatccggc tgagaagagt aacattccgg caaagatcag
    19381 ccacagcgag ctgaataagg tcatcagtaa accaaacagc ataacccccg tcgaaaacaa
    19441 catcaccgga ccgcgcccat agcgggtggt catggttccg gctttgggtg agctccatgt
    19501 accggtcaaa taggccagcg ataataagcc aaccacagcc tgactgacat gccaggggga
    19561 gagcatcaac cgatagccga tgtaattaaa cagcgtgacg aacgacccca tcagcaaaaa
    19621 gccttctgcg aacaataacg gtaatccccg gtcacgccag tgcagacgaa agttgataaa
    19681 caacgtctta ggtcgcagcg aagtcgggcg aaaatggcgt gattcaggga ggattttcca
    19741 gaacattaac gccgaggcca gcgcgacaca accgattgcc gccagcgcaa ttcgccagtt
    19801 gaaaaagtcc gtgaagacac cgctaattaa gcgtccgctc atgccgccaa ttgagttgcc
    19861 gctgatatac aaccccatcg aaaaggccac gaaactgggg tggatttcct cgctaagata
    19921 agtcatgcca acagctgcta cgccacttaa cgaaagccca atcaaggcgc gcataatcaa
    19981 aatgccgtgc cagctggtca tcattgtcga aagtaacgta caaatggagg ccaacagtag
    20041 cgccgtgacc atcactggtt tgcgaccaat ggcatcggat aacgggccag taaacagcaa
    20101 accgatagcc aacatcgccg tggaaatgga cagtgaaata ctactgttcg cgggcgttaa
    20161 gccaaactcc tgcgaaagca ccggaaggat aggttgcaca caatagagaa gtgcgaaggt
    20221 tgccagtccg gcagagaaca gcgccagggt gacgcgcata aattgcggcg taccgcgttt
    20281 aataaattga tttggctgag aaatgctttg cttgtcagtg tcgcttgccg gagcgccatc
    20341 aacagttgta gtacggctca cttgaaatcc ttgctaaata tgcctgtgga tcaggcttat
    20401 ggatagatta ggaaaatcga attgttctgt ctaatatatt aataatctca aataagatgt
    20461 tttaaatatg aatattgaac ttcgtcatct gcgttacttt gttgcggttg cagaagagct
    20521 gcatttcggg cgcgccgctg cccgcctgaa tatttcgcaa ccgccgctaa gccagcagat
    20581 tcaggcgctg gagcaacaaa ttggtgcccg actgctggca cgaaccaatc gcagtgtatt
    20641 tctgacggca gcaggaaaac agtttcttgc agatagtcgg caaatcctgt ctatggtgga
    20701 tgacgctgcc gctcgcgctg aaagactgca tcagggtgaa gcgggggagt tgcgtattgg
    20761 ttttacttcg tccgctcctt ttattcgggc ggtgtctgat acgttatcgc tgtttcgccg
    20821 tgattatccc gatgtccatt tacaaacccg cgaaatgaac actcgcgagc aaatcgctcc
    20881 gctcattgaa ggaacgctgg atatgggatt gctgcgtaac acagcgttac cggagacgct
    20941 tgaacacgca gtcatcgtcc atgaaccgct tatggcgatg atcccgcacg atcatcccct
    21001 ggcaaataac ccgagtgtaa cgctggctga actggcgaaa gaaccctttg tcttttttga
    21061 tccgcacgtc gggacagggc tgtatgacga tattctcgga ctgatgcgac gttaccattt
    21121 gacgcccgtc atcactcagg aggtgggtga ggcaatgacc atcatcggtc tggtttccgc
    21181 tggtctgggc gtttcaattt tgcctgcgtc atttaaacgt gttcagctca acgaaatgcg
    21241 ctgggtgccg attgctgaag aggatgcggt ttctgaaatg tggttggtct ggccgaaaca
    21301 tcatgaacaa agtccagctg cgcgaaactt tcgtattcat ctgctgaatg ctctcaggtg
    21361 agggaaattt cagcgaaaaa gcccgaaaaa tgtgctgtta atcacatgcc taagtaaaaa
    21421 tttgacgaca cgtattgaag tgcttcacca tagcctacag attatttcgg agcgcgaaaa
    21481 tatagggagt atgcggtggt tgctgaaaac cagcctgggc acattgatca aataaagcag
    21541 accaacgcgg gcgcggtata tcgcctgatt gatcagcttg gtccagtctc gcgtatcgat
    21601 ctttcccgtc tggcgcaact ggctcctgcc agtatcacta aaattgtccg tgagatgctc
    21661 gaagcacacc tggtgcaaga gctggaaatc aaagaagcgg ggaaccgtgg ccgtccggcg
    21721 gtggggctgg tggttgaaac tgaagcctgg cactatcttt ctctgcgcat tagtcgcggg
    21781 gagattttcc ttgctctgcg cgatctgagc agcaaactgg tggtggagga ggcgcaggaa
    21841 ctggcgttaa aagatgactc accattgctg gatcgtatca tttcccatat cgatcagttt
    21901 tttatccgcc accagaaaaa acttgagcgt ctaacttcga ttgccataac cttgccggga
    21961 attattgata cggaaaatgg cattgtacat cgcatgccgt tctacgagga tgtaaaagag
    22021 atgccgctcg gcgaggcgct ggagcagcat accggcgtac cggtttatat ccagcatgat
    22081 atcagcgcat ggacgatggc agaggccttg tttggtgcct cacgcggggc gcgcgatgtg
    22141 attcaggtgg ttatcgatca caacgtgggg gcgggcgtca ttaccgatgg tcatctgcta
    22201 cacgccggca gcagtagcct cgtggaaata ggtcacacgc aggtcgaccc gtatgggaaa
    22261 cgctgttatt gcgggaatca cggctgcctc gaaaccatcg ccagtgtgga cagtattctt
    22321 gagctggcac agctgcgtct caatcaatcc atgagctcga tgttacatgg acagccgtta
    22381 accgtggact cattgtgtca ggcggcattg cgcggcgatc tactggcaaa agacatcatt
    22441 accggggtgg gcgcgcatgt cgggcgcatt cttgccatca tggtgaattt atttaaccca
    22501 caaaaaatac tgattggctc accgttaagt aaagcggcag atatcctctt cccggtcatc
    22561 tcggacagca tccgtcagca ggcccttcct gcgtatagtc agcacattag cgttgagagt
    22621 actcaatttt ctaaccaggg tacgatggca ggggctgcgc tagtaaaaga cgcgatgtat
    22681 aacggttctt tgttgattcg tctgttgcag ggttaacatt ttttaactgt tctaccaaaa
    22741 tttgcgctat ctcaatttgg gccaggaaag cataacttag actttcaagg ttaattattt
    22801 tcctggttta tatttgtgaa gcataacggt ggagttagtg atgctgaagc gtttctttat
    22861 taccggtaca gacacttctg tagggaaaac ggtggtttcc cgcgcattgc tacaagcgtt
    22921 agcctcccag ggaaaaacgg ttgcgggata taaacccgta gcgaagggga gcaaagagac
    22981 acccgaaggg ctgcgtaata aagatgccct ggtgttgcag agtgtttcaa ccatcgaact
    23041 gccttatgaa gcagttaatc ctatcgcgtt aagcgaagaa gaaagtagcg tggcgcacag
    23101 ttgcccaatc aattacaccc tcatttcaaa cggcctggca aacctgaccg ataaagtcga
    23161 tcatgtcgtg gtagaaggga ctggcggctg gcgcagtctg atgaatgatt tgcgtccgct
    23221 ctctgaatgg gtggtgcagg aacaactgcc ggtgttgatg gttgtcggta ttcaggaagg
    23281 ttgcattaac catgcactgc taacagctca ggcgattgcc aacgacgggc tgccgctcat
    23341 tggctgggtg gctaaccgaa tcaacccagg acttgcgcat tatgcggaaa tcattgatgt
    23401 gctcggtaaa aaacttccgg caccgctcat tggtgaactg ccttatctgc cgcgcgccga
    23461 acagcgtgaa ctggggcaat acatccgctt agctatgctt cgcagtgtgc tggcggtaga
    23521 tagagtcacg gtgtaacgtc cgcgaaatta ccgacgcaat tacgcaggca atcaataaac
    23581 cggggagtag ctgatactcc ccggtcattt cacatatcat caacgtcgac ataatcggcg
    23641 cgtgcgtggt tgccgccaat agtgtcgcca tcccggtcaa tccgagtaaa agcgtaattt
    23701 cttcgccatc agggaaccat aatcccaggc tacgaccata caacatgcca atggcaagac
    23761 cgataaatag cgtcggtgta aagacaccac cgggcgcgcc agaaccacta ctcgccagca
    23821 cggcaaacag tttacagagg aagatcccgg caatgatcat taacagtggc ggggcggtta
    23881 aaaaggattg tacggtgcta tagccgttgc cccacactgc aggtgtgaac agggaaagca
    23941 gacccacgat caacccgccc agtgccagtt gccagggcgg cgcaagtttg agacttacaa
    24001 atccacgatg acaggcgttc attaacgtta acaacagtgg tccgcacaga cctgccagca
    24061 cacctgtact gataatcagc gcatagtcac gagcctgaac cgtcactgag agttgtacgc
    24121 tgtagagtaa cgcgtcgcta tgattaatca gattgctaac cagcaacgcc acgacggcgg
    24181 aaataatcac cgggccgaga gaggccaaca tcatagtgcc aaacagcact tcggctataa
    24241 ataaactgcc agcaagcggg gctcgatagg ccgcagccat tcccgccgca gctccacagg
    24301 cgatccataa tttccactcc tggcgtggcg taaaacgttg ggcaaaacag gaggcggcaa
    24361 gggcagctaa aagaatcatc gcaccttcgc gaccaattgc actgccgctg gttactacca
    24421 gcagagaggc aagcgattta accaggcttg ctgcgtaatc gaactgacca tcggtttgca
    24481 acgcttccat gtaatcggtc ggcgcatgag ggcgttgctg ggtaaatttc tgccagccca
    24541 tcagcaacaa acccgccgcc agtccgccga gcgccggagt tagcaaccgt cgccatgggg
    24601 aaagatttgt cgctgcattg accagactgc cggagtcatt attgaggaac aaccactcca
    24661 gtagcagcat tgcatgacga aacccggcaa cggcaaaggc cgcaagaata ccgacgactg
    24721 ttgcgataag cagacggcgg aacatagtgc tcagatccgg atagatgtgt agatgatgca
    24781 tgagcagaaa tgggcaatga aacacgggcg actattttgc ggcaaaagag gccagccgca
    24841 aaagcagtgc gtgctttaag agataaaaaa acgtgacact gtaacgtatt attgccgaat
    24901 cgcgcgtaat cgtcaccatc cggcaatatt acggtgatcc tatcgaaaga gtggtttaac
    24961 cgcgacagga atcaacagtt gtgattgcca ttgcgccagc gttaagcgag ccagttcacc
    25021 cagtgcacag tagaaagggt gttctgcttt ttcgataaaa acgtcaagaa aacgtgttga
    25081 ccacggaaaa aggtgccatg ccagcaattc ttcgcattct gtctggcgac cattctccgc
    25141 caaccacgca gccatcaaca gcagtgaacc aaaatgatct tccggttcgt tttgctgcat
    25201 ttcaaactga atgcctttct cgcgcatcca ctgacgaagt gccaatgttg aatcgccaaa
    25261 cagcacagat tcgcgatcca gccagaccga tccccacggc ggtgacggca gtgcccacgg
    25321 gccgacaaac aaacgctgcc aagcctgggc gtgagtctct tcactctgtg tctgaaaagc
    25381 agtcaccagc ggcgctaatg actcttctgg tagaggccac tgagtttccc agccgtcacg
    25441 ggtgagtgcc gcaaccaggg gggcggcttc cgcactctct ggagcgtaat aaaacagcgc
    25501 gcccaacacg cgcgccgcaa cagaaaaatt atcttgctgt gaaaaatggg tcattcctaa
    25561 catcctgaaa tgtgcgggtt gccccgcacc aatttgttaa cctgcaatag ccattcctac
    25621 cgtcatgtgc aggccataaa agagcacacg cccgatcatt tcaccgccaa gaatgaggat
    25681 taaccccaga acaagtccgg caacgtgagg ttcacggcga cgaatgagcg gacaaagcca
    25741 gcatcctaga cctgcgcata acaataccac acgccagacc tgtaaggagg cgtaatctgg
    25801 taccagtgcg ctggcttgtt gtacggatga gtgaatagac gccagagaca aaccttgcag
    25861 cacaatcacc ccggcacaag cgatcagcgc cagaacgcta atgatggcaa atggtgtggt
    25921 attaaatgta acgcgggctg cccgcagaat tgcggcagca agtattggac cgctcaacaa
    25981 tactgtcagg aagaaagcca gcgtagtgta accgttatgc caggttggca ctgtgtcgat
    26041 ttgatacacg caggtcatca tccagacgaa aatgacgccg agcgccatgc tgacaagtaa
    26101 ccagagtttc cccaacgctt gcggcatttt accgatgact gccaccagcc accataatcc
    26161 gccaacggca aaaaatatgg aaccggcagc aatctcgtta ctcagaccag aatctccgat
    26221 tcgattcagc gagttaaacg cccgcagtgg cgaccccagg tgcatgacag aggcaataaa
    26281 cccgatgccc atcaacagcc agaggaaaaa catgccacgg acaatacgct gacggtctgc
    26341 gtcatttttg gcggcgaacc agccaattcc gctaacaatc agcgcgccta ctacgcattg
    26401 acccaatacg gtaaagataa ccagtggcca ttcatgccat ccatttccca ttttacactt
    26461 cctccggatt agccagataa ccagtggtat cgccggtcgg gcggctgttg gcgttaggtt
    26521 tgataacgat attgggtttt gtgaaatgcg cgcgcggcag tggcgcaacg gcagccagag
    26581 tgccgtgctt ctggcgcagc tcttcaattg gaccgaattc aagtgcacgc agcgggcagg
    26641 attcgacaca tatgggttgt ttcccctcgg cgacgcgcga ataacaacca tcgcacttcg
    26701 tcatgtgccc tttttcagca ttgtactgtg gtgcgccgta cggacaagcc atgtggcagt
    26761 agcggcagcc gatacaaaca tcttcatcaa ccaccacaaa gccatcttca cgcttatgca
    26821 ttgcgccgct ggggcagact tttgtacatg cggggtcatc gcaatggtta caggagatgg
    26881 agagataata agcgaacacg ttctggtgcc agacgccatt atcttcctgc cagtcgccgc
    26941 cagcatattc ataaatacgg cggaaactga cttccgggcc aagatcttta aaatctttgc
    27001 acgccagttc gcaggtttta cagccagtac agcggctgga atcgataaaa aatccatatt
    27061 gtgtggtcat gggctactcc ttaaaccttt tcgatctgga ctagattgct gtgcgacggg
    27121 tttccctttg ccagcggtga agggcggtga gaggtcagaa tattgatact gccgccgtga
    27181 tcgacccggt caccaaacat atccgcttta agccacgcac cttgcccgat ggcggtaacg
    27241 ccaggcagaa tacgcggagt cacttttgcg gcaatcagca tttctccatt agtgttaaat
    27301 acccgcacgg tatcgccatg acggataccg cgtgcctgag catcaatggg gttgatccac
    27361 acctcttgtg ggcaggcctg ctgtaacaca tcaatattgc cgtagctgga gtgggtacgt
    27421 gctttgtagt ggaagcccgt tagctgcagt ggataggttt tccgcagggg atcgtcccag
    27481 ccatcaaaac ctggggtata tgcaggaagg ggatgaataa tttcatcttt tttcaattcc
    27541 caggtatccg caatcgtcgc cagtcgttca gaataaattt cgattttccc cgaaggtgtt
    27601 ttcaacgggt ttgcctgtgg atcttcacgg aatgcgcgga aagcgacgta gtgttcttcc
    27661 gggcattttt tcttaaagat cccggtcgtt ttcatctcct cgtagtcggg catctcaggg
    27721 ttacgttcct tcgttttcgc atggagatat ttgatccatt catgctgact gcgaccttca
    27781 gtaaaggttt gataaacgtc tggtcctaag cgtttggcaa cttcactcag aatccagtag
    27841 atgggtttgc gttcaaattt tgctgaggtt gcgggttggg cgaggatcac atagcccata
    27901 ttcccggcag attcgtgaga gataaggtct tcttgctctg ttggcatcag gtcgggcaac
    27961 aggatatcgc aatacttagc cgaggcagtc atgaagtggt caatgccaac aatcatctcg
    28021 cacttgctgt catcctgaag cacctcatgg gtgtgattga tgtcgccatg ttgattgatc
    28081 aatgtgttac tggcgtagca ccataaaaac ttgatgggga catccagttt ttcttttcca
    28141 cgaacaccat cacgggtcgc ggtcatttcc gtaccatggt cgatggcatc tgtccatgt
//
LOCUS       DAMZFT010000058        27920 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.058, whole genome shotgun sequence.
ACCESSION   DAMZFT010000058 DAMZFT010000000
VERSION     DAMZFT010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 27920)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27920)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27920
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.058"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..197
                     /locus_tag="PN977_002580"
     CDS             <1..197
                     /locus_tag="PN977_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001615141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HDI6042599.1"
                     /translation="DDNRVKVNNDGNFVNDLSGRRGIYQAGIKASFSSSLSGHLGVGY
                     SHGAGVESPWNAVAGANWSF"
     gene            complement(564..830)
                     /gene="minE"
                     /locus_tag="PN977_002581"
     CDS             complement(564..830)
                     /gene="minE"
                     /locus_tag="PN977_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HDI6042600.1"
                     /translation="MALLDFFLSRKKNTANIAKERLQIIVAERRRSDAEPHYLPQLRK
                     DILEVICKYVQIDPEMVTVQLEQKDGDISILELNVTLPEAEELK"
     gene            complement(834..1646)
                     /gene="minD"
                     /locus_tag="PN977_002582"
     CDS             complement(834..1646)
                     /gene="minD"
                     /locus_tag="PN977_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020801817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HDI6042601.1"
                     /translation="MARIIVVTSGKGGVGKTTSSAAIATGLAQKGKKTVVIDFDIGLR
                     NLDLIMGCERRVVYDFVNVIQGDATLNQALIKDKRTENLYILPASQTRDKDALTREGV
                     AKVLDDLKAMDFEFIVCDSPAGIETGALMALYFADEAIITTNPEVSSVRDSDRILGIL
                     ASKSRRAENGEEPIKEHLLLTRYNPGRVSRGDMLSMEDVLEILRIKLVGVIPEDQSVL
                     RASNQGEPVILDINADAGKAYADTVERLLGEERPFRFIEEEKKGFLKRLFGG"
     gene            complement(1670..2365)
                     /gene="minC"
                     /locus_tag="PN977_002583"
     CDS             complement(1670..2365)
                     /gene="minC"
                     /locus_tag="PN977_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HDI6042602.1"
                     /translation="MSNTPIELKGSSFTLSVVHLHEAEPKVIHQALEDKIAQAPAFLK
                     HAPVVLNVSALEDPVNWSAMHKAVSATGLRVIGVSGCKDAQLKAEIEKMGLPILTEGK
                     EKAPRPAPAPQAPAQNTTPVTKTRLIDTPVRSGQRIYAPQCDLIVTSHVSAGAELIAD
                     GNIHVYGMMRGRALAGASGDRETQIFCTNLMAELVSIAGEYWLSDQIPAEFYGKAARL
                     QLVENALTVQPLN"
     gene            2885..3253
                     /locus_tag="PN977_002584"
     CDS             2885..3253
                     /locus_tag="PN977_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgJ family protein"
                     /protein_id="HDI6042603.1"
                     /translation="MNMMRIFYIGLSGVGMMFSSMASGNDAGGLQSPACGVVCDPYIC
                     VNSDGISPELTRKYLGEKAAENLQSVQGYDPSEFTFSNGVFCDVKEKLCRDDRYFGVD
                     GKRSGKINQTTTKMLFMCRE"
     gene            complement(3356..3757)
                     /locus_tag="PN977_002585"
     CDS             complement(3356..3757)
                     /locus_tag="PN977_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPC domain-containing protein"
                     /protein_id="HDI6042604.1"
                     /translation="MKIKGISKAVLLLALLTSTSFAAGKNVNVEFRKGHSSAQYSGEI
                     KGYDYDTYTFYAKKGQKVHVSISNEGADTYLFGPGIDDSVDLSRYSPELDSHGQYSLP
                     ASGKYELRVLQTRNDARKNKTKKYNVDIQIK"
     gene            4000..4293
                     /locus_tag="PN977_002586"
     CDS             4000..4293
                     /locus_tag="PN977_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgL domain-containing protein"
                     /protein_id="HDI6042605.1"
                     /translation="MFCVIYRSSKRDQTYLYVEKKDDFSRVPEELMKGFGQPQLAMIL
                     PLDGRKKLVNADIEKVKQALTEQGYYLQLPPPPEDLLKQHLSVMGQKTDDTNK"
     gene            4365..5024
                     /locus_tag="PN977_002587"
     CDS             4365..5024
                     /locus_tag="PN977_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HDI6042606.1"
                     /translation="MYQHHNWQGALLDYPVSKVVCVGSNYAKHIKEMGSAVPEEPVLF
                     IKPETALCDLRQPLAIPSDFGSVHHEVELAVLIGATLRQATEEHVRKAIAGYGVALDL
                     TLRDVQGKMKKAGQPWEKAKAFDNSCPLSGFIPAAEFTGDPQNTTLGLSVNGEQRQQG
                     TTVDMIHKIVPLIAYMSKFFTLKAGDVVLTGTPDGVGPLQSGDELTVTFDGHSLTTRV
                     L"
     gene            5116..5562
                     /locus_tag="PN977_002588"
     CDS             5116..5562
                     /locus_tag="PN977_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309703.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgN family cysteine cluster protein"
                     /protein_id="HDI6042607.1"
                     /translation="MSDVPFWQSKTLDEMSDAEWESLCDGCGQCCLHKLMDEDTDEIY
                     FTNVACRQLNIKTCQCRNYERRFEFEPDCIKLTRENLPTFEWLPMTCAYRLLAEGKDL
                     PAWHPLLTGSKAAMHGERISVRHIAVKESEVIDWQDHILNKPDWAQ"
     gene            complement(5769..6680)
                     /gene="hlyE"
                     /locus_tag="PN977_002589"
     CDS             complement(5769..6680)
                     /gene="hlyE"
                     /locus_tag="PN977_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin HlyE"
                     /protein_id="HDI6042608.1"
                     /translation="MTEIVADKTVEVVKNAIETADGALDLYNKYLDQVIPWQTFDETI
                     KELSRFKQEYSQAASVLVGDIKTLLMDSQDKYFEATQTVYEWCGVATQLLAAYILLFD
                     EYNEKKASAQKDILIKVLDDGITKLNEAQKSLLVSSQSFNNASGKLLALDSQLTNDFS
                     EKSSYFQSQVDKIRKEAYAGAAAGVVAGPFGLIISYSIAAGVVEGKLIPELKNKLKSV
                     QSFFTTLSNTVKQANKDIDAAKLKLTTEIAAIGEIKTETETTRFYVDYDDLMLSLLKE
                     AAKKMINTCNEYQKRHGKKTLFEVPEV"
     gene            7052..7471
                     /locus_tag="PN977_002590"
     CDS             7052..7471
                     /locus_tag="PN977_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="HDI6042609.1"
                     /translation="MLFIKPADLREIVTFPLFSDLVQCGFPSPAADYIEQRIDLNQLL
                     IQHPSATYFVKASGDSMIDGGISDGDLLIVDSAITASHGDIVIAAVDGEFTVKKLQLR
                     PTVQLIPMNSAYSPITISSEDTLDVFGVVIHVVKAMR"
     gene            7471..8739
                     /gene="umuC"
                     /locus_tag="PN977_002591"
     CDS             7471..8739
                     /gene="umuC"
                     /locus_tag="PN977_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase V catalytic protein"
                     /protein_id="HDI6042610.1"
                     /translation="MFALCDVNAFYASCETVFRPDLWGKPVVVLSNNDGCVIARNAEA
                     KALGVKMGDPWFKQKDLFRRCGVVCFSSNYELYADMSNRVMSTLEELSPRVEIYSIDE
                     AFCDLTGVRNCRDLTDFGREIRATVLQRTHLTVGVGIAQTKTLAKLANHAAKKWQRQT
                     GGVVDLSNLERQRKLMSALPVDEVWGIGRRISKKLDAMGIKTVLDLADTDIRFIRKHF
                     NVVLERTVRELRGEPCLQLEEFAPTKQEIICSRSFGERITDYTSMRQAICSYAARAAE
                     KLRSEHQYCRFISTFIKTSPFALNEPYYGNSASVKLLTPTQDSRDIINAATRSLDAIW
                     QAGHRYQKAGVMLGDFFSQGVAQLNLFDDNAPRPGSEQLMAVMDTLNAKEGRGTLYFA
                     GQGIQQQWQMKRAMLSPRYTTRSSDLLRVK"
     gene            complement(8785..9315)
                     /gene="dsbB"
                     /locus_tag="PN977_002592"
     CDS             complement(8785..9315)
                     /gene="dsbB"
                     /locus_tag="PN977_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein DsbB"
                     /protein_id="HDI6042611.1"
                     /translation="MLRFLNQCSQGRGAWLLMAFTALALELTALWFQHVMLLKPCVLC
                     IYERCALFGVLGAALIGAIAPKTPLRYVAMVIWLYSAFRGVQLTYEHTMLQLYPSPFA
                     TCDFMVRFPEWLPLDKWVPQVFVASGDCAERQWDFLGLEMPQWLLGIFIAYLIVAVLV
                     VISQPFKAKKRDLFGR"
     gene            complement(9461..11002)
                     /gene="nhaB"
                     /locus_tag="PN977_002593"
     CDS             complement(9461..11002)
                     /gene="nhaB"
                     /locus_tag="PN977_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)/H(+) antiporter NhaB"
                     /protein_id="HDI6042612.1"
                     /translation="MEISWGRALWRNFLGQSPDWYKLALIIFLILNPLIFIISPFVAG
                     WLLVAEFIFTLAMALKCYPLLPGGLLAIEAVFIGMTSAEHVREEVAANLEVLLLLMFM
                     VAGIYFMKQLLLFIFTRLLLSIRSKMLLSLSFCMAAAFLSAFLDALTVVAVVISVAVG
                     FYGIYHRVASSRTEDTDLQDDSHIDKHYKVVLEQFRGFLRSLMMHAGVGTALGGVMTM
                     VGEPQNLIIAKAAGWHFGDFFLRMSPVTVPVLICGLLTCLLVEKLRCFGYGETLPEKV
                     REVLQQFDDQSRHQRTRQDKIRLIVQAIIGVWLVTALALHLAEVGLIGLSVIILATSL
                     TGVTDEHAIGKAFTESLPFTALLTVFFSVVAVIIDQQLFSPIIHFVLQASEHAQLSLF
                     YIFNGLLSSISDNVFVGTIYINEAKAAMESGAITLKQYELLAVAINTGTNLPSVATPN
                     GQAAFLFLLTSALAPLIRLSYGRMVWMALPYTLVLTLVGLLCVEFTLAPVTEWFMQMG
                     WIATL"
     gene            11225..11944
                     /gene="fadR"
                     /locus_tag="PN977_002594"
     CDS             11225..11944
                     /gene="fadR"
                     /locus_tag="PN977_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid metabolism transcriptional regulator
                     FadR"
                     /protein_id="HDI6042613.1"
                     /translation="MVIKAQSPAGFAEEYIIESIWNNRFPPGTILPAERELSELIGVT
                     RTTLREVLQRLARDGWLTIQHGKPTKVNNFWETSGLNILETLARLDHESVPQLIDNLL
                     SVRTNISTIFIRTAFRQHPDKAQEVLATANEVADHADAFAELDYNIFRGLAFASGNPI
                     YGLILNGMKGLYTRIGRHYFANPEARSLALGFYHKLSALCSEGAHDQVYETVRRYGHE
                     SGEIWHRMQKNLPGDLAIQGR"
     gene            complement(11996..13528)
                     /locus_tag="PN977_002595"
     CDS             complement(11996..13528)
                     /locus_tag="PN977_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoVR family protein"
                     /protein_id="HDI6042614.1"
                     /translation="MATIDSMNKDTTRLSDGPDWTFDLLDVYLAEIDRVAKLYRLDTY
                     PHQIEVITSEQMMDAYSSVGMPINYPHWSFGKKFIETERLYKHGQQGLAYEIVINSNP
                     CIAYLMEENTITMQALVMAHACYGHNSFFKNNYLFRSWTDASSIVDYLIFARKYITEC
                     EERYGVDEVERLLDSCHALMNYGVDRYKRPQKISLQEEKARQKSREEYLQSQVNMLWR
                     TLPKREEEKTVAEARRYPSEPQENLLYFMEKNAPLLESWQREILRIVRKVSQYFYPQK
                     QTQVMNEGWATFWHYTILNHLYDEGKVTERFMLEFLHSHTNVVFQPPYNSPWYSGINP
                     YALGFAMFQDIKRICQSPTEEDKYWFPDIAGSDWLDTLHFAMRDFKDESFISQFLSPK
                     VMRDFRFFTVLDDDRHNYLEISAIHNEEGYREIRNRLSSQYNLSNLEPNIQIWNVDLR
                     GDRSLTLRYIPHNRAPLDRGRKEVLKHVHRLWGFDVMLEQQNEDGSVELLERCPPRMG
                     NL"
     gene            13858..15156
                     /gene="dadA"
                     /locus_tag="PN977_002596"
     CDS             13858..15156
                     /gene="dadA"
                     /locus_tag="PN977_002596"
                     /EC_number="1.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid dehydrogenase"
                     /protein_id="HDI6042615.1"
                     /translation="MRVVILGSGVVGVASAWYLNQAGHEVTVIDREPGAALETSAANA
                     GQISPGYAAPWAAPGVPLKAIKWMFQRHAPLAVRLDGTQFQLKWMWQMLRNCDTSHYM
                     ENKGRMVRLAEYSRDCLKALRAETNIQYEGRQGGTLQLFRTEQQYENATRDIAVLEDA
                     GVPYQLLESSRLAEVEPALAEVAHKLTGGLQLPNDETGDCQLFTQNLARMAEQAGVKF
                     RFNTPVDQLLCDGEQIYGVKCGDEVIKADAYVMAFGSYSTAMLKGIVDIPVYPLKGYS
                     LTIPIAQEDGAPVSTILDETYKIAITRFDNRIRVGGMAEIVGFNTELLQPRRETLEMV
                     VRDLYPRGGHVEQATFWTGLRPMTPDGTPVVGRTRFKNLWLNTGHGTLGWTMACGSGQ
                     LLSDLLSGRTPAIPYEDLSVARYSRGFTPSRPGHLHGAHS"
     gene            15166..16236
                     /gene="dadX"
                     /locus_tag="PN977_002597"
     CDS             15166..16236
                     /gene="dadX"
                     /locus_tag="PN977_002597"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309712.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolic alanine racemase DadX"
                     /protein_id="HDI6042616.1"
                     /translation="MTRPIQASLDLQALKQNLSIVRQAAPRARVWSVVKANAYGHGIE
                     RIWSALGATDGFALLNLEEAITLRERGWKGPILMLEGFFHAQDLEIYDQHRLTTCVHS
                     NWQLKALQNARLKAPLDIYLKVNSGMNRLGFQPDRVLTVWQQLRAMANVGEMTLMSHF
                     AEAEHPDGISSAMARIEQAAEGLECRRSLSNSAATLWHPEAHFDWVRPGIILYGASPS
                     GQWRDIANTGLRPVMTLSSEIIGVQTLKAGERVGYGGRYTARDEQRIGIVAAGYADGY
                     PRHAPTGTPVLVDGVRTMTVGTVSMDMLAVDLTPCPQAGIGTPVELWGKEIKIDDVAA
                     AAGTVGYELMCALALRVPVVTV"
     gene            complement(16406..17842)
                     /locus_tag="PN977_002598"
     CDS             complement(16406..17842)
                     /locus_tag="PN977_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016243131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6042617.1"
                     /translation="MASFKDICPFAPAIPPSSVNPGVRRPPGRLFLSAELKNQILQNM
                     KYLSPQKFSWGDAPWQIIDLSTAGKVNIQVDNNTIITLGTPLNQQHNEFMMVAKWCEW
                     AIQQDGLQENLQKKLHEVLEENRQNKQSEIPQEDLKERLKEIKEDILKENQSASQIED
                     RAEALRRMKECLITRQSMLDLSNLGLTSLPENLPPHLIEFNCSRNMLTALPEVMPKGL
                     RVLECMENFLILLPKVQPPKLMVLKCYENYIIWLPELSTNLRVIDCSENFLQFLPPSM
                     PQYLYTLRCAFNSISLIPDEMLENLTRLKVFDCSSNDLISSPRLPPKLIIYYCGENQF
                     KTVPVPQPRSLKVFSCNGNPWDKDNLPTLLKAVEGLKNQEGLEELLDFLHKEGLVDLE
                     GLEELEDLDDLMDLEFLDDPELLERVKVQEDLELLDQQLGLLSLEKQQDSQPVNQQSE
                     HEPESASKVKRDLSEVDSESTMKRKRFM"
     gene            complement(18181..18342)
                     /locus_tag="PN977_002599"
     CDS             complement(18181..18342)
                     /locus_tag="PN977_002599"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6042618.1"
                     /translation="MDDKPLQALANELKTPAQESQRKGFFRLQQVKKNQPRITGIPVN
                     HQGPLPDIA"
     gene            complement(18462..20198)
                     /locus_tag="PN977_002600"
     CDS             complement(18462..20198)
                     /locus_tag="PN977_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HDI6042619.1"
                     /translation="MDATTIISLFILGSILVTSSILLSSFSSRLGIPILVIFLAIGML
                     AGVDGVGGIPFDNYPFAYMVSNLALAIILLDGGMRTQASSFRVALGPALSLATLGVLI
                     TSGLTGMMAAWLFNLDLIEGLLIGAIVGSTDAAAVFSLLGGKGLNERVGSTLEIESGS
                     NDPMAVFLTITLIAMIQQHESSVSWMFVVDILQQFGLGIVIGLGGGYLLLQMINRIAL
                     PAGLYPLLALSGGILIFALTTALEGSGILAVYLCSFLLGNRPIRNRYGILQNFDGLAW
                     LAQIAMFLVLGLLVNPSDLLPIAIPALILSAWMIFFARPLSVFAGLLPFRGFNLRERV
                     FISWVGLRGAVPIILAVFPMMAGLENARLFFNVAFFVVLVSLLLQGTSLSWAAKKAKV
                     VVPPVGRPVSRVGLDIHPENPWEQFVYQLSADKWCVGAALRDLHMPKETRIAALFRDN
                     QLLHPTGSTRLREGDVLCVIGRERDLPALGKLFSQSPPVALDQRFFGDFILEASAKYA
                     DVALIYGLEDGREYRDKQQTLGEIVQQLLGAAPVVGDQVEFAGMIWTVAEKEDNEVLK
                     IGVRVAEEEAES"
     gene            complement(20293..21207)
                     /gene="ldcA"
                     /locus_tag="PN977_002601"
     CDS             complement(20293..21207)
                     /gene="ldcA"
                     /locus_tag="PN977_002601"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="muramoyltetrapeptide carboxypeptidase"
                     /protein_id="HDI6042620.1"
                     /translation="MSLFHLIAPSGYCIKQHAALRGIQRLTDAGHQVNNVEVIARRCE
                     RFAGTETERLEDLNSLARLTTPNTIVLAVRGGYGASRLLADIDWQALVARQQHDPLLI
                     CGHSDFTAIQCGLLAHGNVITFSGPMLVANFGADELNAFTEHHFWLALRNETFTIEWQ
                     GEGPTCQTEGTLWGGNLAMLISLIGTPWMPQIENGILVLEDINEHPFRVERMLLQLYH
                     AGILPRQKAIILGSFSGSTPNDYDAGYNLESVYAFLRSRLSTPLITGLDFGHEQRTVT
                     LPLGAHAILNNTHEGTQLTINGHPVLKM"
     gene            21307..21918
                     /gene="emtA"
                     /locus_tag="PN977_002602"
     CDS             21307..21918
                     /gene="emtA"
                     /locus_tag="PN977_002602"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     EmtA"
                     /protein_id="HDI6042621.1"
                     /translation="MKLRWFAFLIVLLAGCSSKHDYTNPPWNAKVPVQRAMQWMPISQ
                     KAGAAWGVDPQLITAIIAIESGGNPNAVSKSNAIGLMQIKASTSGRDVYRRMGWSGEP
                     TTSELKNPERNISMGAAYLNILETGPLAGIEDPKVLQYALVVSYANGAGALLRTFSSD
                     RKKAISKINDLDADEFLDHVARNHPAPQAPRYIYKLEQALDAM"
     gene            complement(21920..22654)
                     /locus_tag="PN977_002603"
     CDS             complement(21920..22654)
                     /locus_tag="PN977_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar brake protein"
                     /protein_id="HDI6042622.1"
                     /translation="MSHYHEQFLKQNPLAVLGVLRDLHKAAIPLRISWNGGQLISKIL
                     AITPDKLVLDFGSQAEDNIAVLKAQHITITAETQGAKVEFTVEQLQQSEYLQLPAFIT
                     VPPPTLWFVQRRRYFRISAPLHPPYFCQTKLADNSTLRFRLYDLSLGGMGALLETAKP
                     AGLHEGMRFAQIEVNMGQWGVFHFDAQLISISERKVIDGKNETITTPRLSFRFLNVSP
                     TVERQLQRIIFSLEREARKKADKVRD"
     gene            22855..23109
                     /locus_tag="PN977_002604"
     CDS             22855..23109
                     /locus_tag="PN977_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HDI6042623.1"
                     /translation="MGIIAWIIFGLIAGIIAKLIMPGRDGGGFFLTCILGIVGAVVGG
                     WLATMFGIGGSISGFNLHSFLVAVVGAILVLGVFRLLRRE"
     gene            complement(23159..25129)
                     /locus_tag="PN977_002605"
     CDS             complement(23159..25129)
                     /locus_tag="PN977_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HDI6042624.1"
                     /translation="MRLKKRYLCTALSLAFTQQAVAAQESDTLTVWSSPVSSTTTTVL
                     DQPTMKALDKQNVAQALSVVPGVVLQKSGSRNEEQVKVRGFDSRQVPVYFDGVPIYVP
                     YDGNLDLARILTNNLGAVEVSKGYSSLLQGPNQMGGAINITTQKPTKPLEANLGYRQG
                     WSRSQDNAYDMHASFAASSDLGYLQVSGSQLKQDFLGLPHGVNNDIAGKHGKMINSSA
                     DDKRGIVKLGFTPRENDEYTLTYIKQDGEKDNPPYSGNSGQKSRYWQWPEYDKESFYY
                     QGTTQLNDRFTLKSRLYRDTFENTLMMYNSLADLKNKKGSYSHYSDYSDGAGLQLAAD
                     VRENDLLSFAVNWKDDVHREKGAPHAAYDRYEDRTWSLASEYQWAAADNVDVVAGISY
                     DWRDSVEAKKHEKDGSITHYDDNNQSAFNWQVMGKYHFANEDTLALSYYDRTRFPTLK
                     ERYTTSKPAYNQIAIVNPQLKPERARGVDLTWNGAFTHDWGFEVSVYYNRVSDAILSH
                     NIDADTIQNQNSGTVDYSGLDAGIKGKISNILDVGLSYALIHADAKRKDIGKITDLPT
                     QTMTAWMTLKPWEPLSVTLSEEARSSSYSNSDGSQKAAGFAVTHIRADYTLGHGFSVN
                     ASVNNLFDTKYAYSEGFIEEGRNFWAGIAYTF"
     gene            complement(25155..26009)
                     /locus_tag="PN977_002606"
     CDS             complement(25155..26009)
                     /locus_tag="PN977_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ModD protein"
                     /protein_id="HDI6042625.1"
                     /translation="MIFLSQAQIDALLLEDIQGGDLTTRALNIGHQHGYIEFFHRQGG
                     CVSGISVACKMLTTLGLTIDNAVSDGSQANAGQRLIRAQGNAAALHQGWKAVQNVLEW
                     SCGVSDYLAQMLALLRERYPDGNIACTRKAIPGTRLLASQAILAAGGLIHRAGCAETI
                     LLFANHRHFLHDNQDWSGAINQLRRHAPEKKIVVEADTPKEAIAALRAQPDVLQLDKF
                     NPQQATEIAQIAPSLAPHCTLALTGGINLTTLKNYLDCGIRLFITSAPYYATPADIKV
                     SLQPAVSI"
     gene            complement(26006..26818)
                     /locus_tag="PN977_002607"
     CDS             complement(26006..26818)
                     /locus_tag="PN977_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000903984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HDI6042626.1"
                     /translation="MLIDDIDFADLYLQQLRLAHRTEKTPDHWDQRAEKMAENCASPT
                     DSYLQQLTAKIDLQGAQTLFDMGCGPGTVSLALADKLTTIYGVDYSQGMLNVAARRAA
                     ALKADNVHWIQRAWEEDWSDLPRCDIAVASRSTLVADMRQAMSKLNNQARLRVYTTHL
                     VSTSFVSPAIQRAAGREVIELPNYIFALNVLYQMGIYAHVDFIRGQNCQQDNSTWERF
                     EQNVSWSLGALNDDERERLYHWYQQQDARALAPASRDWALIWWDSVPQEALR"
     gene            complement(26828..27586)
                     /locus_tag="PN977_002608"
     CDS             complement(26828..27586)
                     /locus_tag="PN977_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDI6042627.1"
                     /translation="MTLLAVRHASLGYSRHPVLRDVSFTLSQGTICCLLGANGCGKTT
                     LMRSILGVIPLLKGEILLDSLPVQTLSHRQRAQAIAWVPQAHDGIFAFSVLDMVLMGL
                     APTIGAFSVPGKQERLKATEQLEKLGILHLATRRWNTLSGGERQLALIARALVQQPRL
                     LLLDEPASSLDFGHQIQLLDTLAQLKNNGMTMLMSTHHPLHANAIADSIIQVEPDGRV
                     TQGLPTEQLTTKKLAALYRVSADQIHHHLSAIKH"
     gene            complement(27583..>27920)
                     /locus_tag="PN977_002609"
     CDS             complement(27583..>27920)
                     /locus_tag="PN977_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HDI6042628.1"
                     /translation="LSDDEARSLGLNVTRLRFGLIVCATLITASTVAIAGIIGWVGLV
                     VPHIARLLTGHNHQQLLPMAMCTGAILLLLTDTLARSIGTTEIPLGILTAFVGAPFFL
                     FLLLRGGRQ"
BASE COUNT         6959 a         6784 c         7131 g         7046 t
ORIGIN      
        1 tcgatgataa ccgggtgaaa gtgaataatg acggtaattt tgtcaatgat ttgtcgggca
       61 gacgtggaat atatcaggca ggtattaaag cctcattcag cagttcgtta agcgggcatc
      121 tcggggtggg gtatagccat ggtgccggtg tggaatcccc gtggaacgcg gtggctggtg
      181 cgaactggtc gttctgacca tcaacgatta aactgcgctt cggcgtagtt ttcgtttaca
      241 gggtgttaaa agggaaaatt ctgcggcaaa aaaagcccgc cagttacggc gggaaacctc
      301 atcctatggg agaacaatga ataatgaaat tgcggggtta tcatctccca gtatacccat
      361 actaacaata aggttattta ctcagccagg tataaacatt ttgttctgtg tggggggaac
      421 ggcattaagg tgtaaagggg ggggaagcaa tgagtagcat cagcaagaat actcgccgct
      481 cttatcaaac gtggatgagg gggatgaaaa actcaaggca gagatgactc tgccttgaag
      541 ataaatgcgc ttttacagcg ggcttatttc agctcttctg cttccggcaa ggtcacgttc
      601 agctcaagaa tagaaatatc gccatctttt tgctcaagct gtacggttac catctcagga
      661 tcaatttgta cgtatttaca aatgacctca agaatatctt tacgcaactg cggcagatag
      721 tgcggttctg catcgctgcg acggcgttca gcaacaataa tctgcagccg ttcttttgca
      781 atgttggccg tgtttttctt ccgcgagaga aagaaatcga gtaatgccat aacttatcct
      841 ccgaacaagc gtttgaggaa gcctttcttc tcttcttcaa tgaagcggaa aggacgttct
      901 tctcccaaca gacgttctac ggtatctgcg taggctttac ccgcatcggc gttaatgtcg
      961 agaatgaccg gttcaccctg gttagaggcg cgcaatactg attgatcctc agggatcacg
     1021 ccgacgagtt tgatgcgcag gatttccagc acatcttcca tgctcagcat gtcacctctg
     1081 cttacgcggc ctgggttata gcgcgttaac agcaggtgct ctttaatagg ctcttcgcca
     1141 ttttctgcgc ggcgtgattt cgacgccaga atgcctaaaa tacggtcaga gtcgcgtact
     1201 gaggagactt ccgggttggt ggtaataatg gcttcgtctg caaaatagag tgccattagc
     1261 gcaccggttt caatccctgc cggggagtcg caaacgataa attcaaaatc catcgctttc
     1321 agatcatcaa gaactttggc gaccccttca cgggtgaggg catctttatc gcgagtttgc
     1381 gatgccggaa gaatatagag attttcagta cgcttatctt taattaacgc ctgatttagc
     1441 gttgcatcgc cctgaatgac gttgacgaaa tcgtaaacga cccgacgttc acaacccata
     1501 atcaggtcga gattacgcag gccgatatca aaatctatca cgacagtttt ctttcccttc
     1561 tgggccaaac cagtggcgat ggccgcgctg gaggttgtct taccaacacc ccctttgccc
     1621 gaagtaacaa caataatgcg tgccatagaa attccttgtt aaaaagggat caatttaacg
     1681 gttgaacggt caaagcgttt tcaactaact gcagtcgtgc cgctttgcca taaaattctg
     1741 ctgggatttg atcactcagc cagtattcac ctgcgatgga caccagttcc gccatcaggt
     1801 tcgtacaaaa tatttgcgtt tcccggtcac cacttgcccc tgccagcgca cgaccgcgca
     1861 tcatgccata gacatgaatg ttaccatcgg caatcaattc ggccccagcg ctaacgtggc
     1921 ttgtaacaat cagatcacat tgtggagcat aaatacgctg accggaacgc accggggtat
     1981 ctattaaacg cgtttttgtg accggcgttg tattttgcgc tggagcctgc ggtgcgggag
     2041 ctggacgcgg cgctttttcc tttccttccg tcaggatagg cagccccatt ttttcaattt
     2101 cggctttaag ttgcgcatct ttgcagccac ttacgccaat aacccgcaaa ccggttgccg
     2161 aaaccgcctt atgcatcgct gaccagttta ccgggtcttc cagtgcactg acgttgagta
     2221 caacgggggc atgttttaaa aatgcggggg cctgagcgat tttgtcttcc agcgcctgat
     2281 ggataacctt aggttctgcc tcatgcagat gaaccacaga taaagtgaag ctactgcctt
     2341 taagctcgat tggcgtgttt gacatcctgg ccttactcaa ttagctatta atcatcgcca
     2401 gcgcgcgatg atgttccgaa gactataagg catgttatag tctggattat attgaggcaa
     2461 gtcaccctcc catttattca gagtaaaagt ctattctgtg ataaatggcg ctgatacata
     2521 gcttaaaaaa tacccttgtc aatcaaccca ttgccgtcgt acttttgatt gttcttattt
     2581 acgcttcctt tttccgcacc ctaatcgtaa cagcatcttc agacctgttt aaatatccag
     2641 tattgaggcg agtgtatttt ttcaccacgc caggctttct taactttcca ttttttgcgg
     2701 catcattaaa gaacagatac ccgttatctt aatatccgcc acagaattgc tctatcatag
     2761 aaaccgcaga agacgtctga tacttctgtc aggtcatcgt aactcgtcca ggtcagcggt
     2821 tttgattacg gattgatgat gaaatgagca taagtgttgt acggttggat tatcaggatg
     2881 tgctatgaat atgatgcgta ttttttatat cggattgtca ggtgtgggga tgatgttctc
     2941 atcaatggcg tctggaaatg acgctggcgg acttcaatct ccggcgtgcg gcgttgtttg
     3001 cgacccgtat atttgtgtta actcagatgg catttcccca gagttaacaa ggaaatatct
     3061 cggcgaaaaa gccgctgaaa acttacaatc agtacaaggt tacgatccca gcgaatttac
     3121 attctctaac ggtgtatttt gcgatgttaa agaaaaatta tgtcgtgatg atcgttattt
     3181 tggtgtagat ggtaagcgga gtggaaaaat caatcaaacc accacaaaga tgttatttat
     3241 gtgtcgtgag tgatgtcttg tttacatgat gttctattcg tcattaacct cttgagcgac
     3301 agagaggagt ggggcgttaa cgtttatgac ggggagagtc cccgtcggtt gacatttact
     3361 taatttgaat atcgacgtta tattttttag ttttgttttt acgggcgtcg tttcgggttt
     3421 gcagcactcg cagctcatat ttgccagaag cagggagtga gtattgtccg tgactatcga
     3481 gttctgggga atatctggat aaatcaacag aatcatcgat tcctggcccg aagaggtaag
     3541 tatcagcgcc ttcattagag atactcacat gcactttctg accttttttg gcatagaagg
     3601 tatatgtatc gtaatcgtat ccctttattt cgccggagta ctgagcgctg ctatggcctt
     3661 ttctgaactc aacattgaca ttcttacccg cagcaaagct cgtggaggtt agcagtgcaa
     3721 gtaacaatac agccttactg atgcccttaa ttttcattgg tagtctcctg ttcagaatga
     3781 gtacgatgaa ctgttataat ataacaattc atcacaggag aattccagtg gctttcctgc
     3841 gttcggttaa atcccggcgc ggcatttttg ccggggggga ttacttataa agggggcatg
     3901 gactgaacaa accgtaggaa tctacagctg gctggcttgt ggcgcgggtt tcattgatgt
     3961 tagcttatgc caaaacctgg catcttaaag agtaaaagta tgttttgtgt gatttatcga
     4021 agcagcaagc gtgatcagac ctatttatat gtcgaaaaaa aagacgattt ttcgcgtgtt
     4081 cctgaggaac tgatgaaagg ttttggtcag cctcagttag cgatgattct tccgctggat
     4141 gggcggaaga aactggtcaa tgccgatatt gaaaaggtta aacaggcatt aaccgagcaa
     4201 ggttactatt tgcagttacc gccaccaccc gaagatttgc tgaagcaaca tctttccgtc
     4261 atggggcaga aaacagacga cacgaacaaa taaccgatat ccggcggtgg cattatcttt
     4321 gtcggcgcgg gttttcatat ccacgataag gtgaggggaa cgttatgtat caacatcaca
     4381 actggcaagg tgcgctgctg gattatccgg tgagtaaagt agtctgtgtt ggcagtaact
     4441 atgccaaaca tattaaagag atgggcagcg cagtgcccga agagccagtg ctgtttatta
     4501 aaccagaaac ggcactgtgc gatctgcggc agccattggc gatcccatcc gatttcggtt
     4561 cagttcatca tgaagtcgaa ctggcggtgt tgattggcgc gacgctgcgt caggctacgg
     4621 aagagcatgt ccgcaaagcc attgccggtt acggcgtggc gcttgatctg acattgcgtg
     4681 atgttcaggg aaaaatgaag aaagccgggc agccgtggga aaaggctaaa gcgtttgata
     4741 actcttgtcc gctttccggg tttattcccg cggcggaatt taccggcgat ccacaaaata
     4801 caacgctggg cctgagcgta aacggcgaac aacgccagca aggtacgact gtggacatga
     4861 tccataaaat cgttccgctg atcgcttata tgagcaagtt ctttaccctc aaggccggtg
     4921 acgttgtgct gacaggcacg cctgatggcg tcggcccgtt gcaaagcggt gatgagctga
     4981 cagtcacttt cgatgggcat tctttgacaa ctcgcgtttt gtaatacttt ttgccgcctg
     5041 aaagcggcgg caaaacttgc atcgctgtgc cagactggtt ataaggtgcg ttttaacgta
     5101 atggcggaac acctgatgag cgatgtacct ttctggcaaa gtaaaaccct ggacgaaatg
     5161 agcgatgcgg aatgggagtc gttgtgtgat ggttgcggtc agtgttgcct gcataaactg
     5221 atggatgaag acaccgacga aatctacttc actaacgtcg cctgtcgcca gctcaatatt
     5281 aaaacctgtc aatgtcggaa ctacgaacgt cgtttcgagt ttgaacctga ctgcattaaa
     5341 ttaacccgtg aaaatctgcc aacattcgaa tggctgccaa tgacctgcgc ttatcgcttg
     5401 ctggcggaag gtaaagattt acctgcgtgg catccgctgc ttactggttc gaaagcggca
     5461 atgcatggcg aacgtatctc tgtgcgtcat atcgcagtga aagaatcaga agtgattgac
     5521 tggcaggatc atatcttaaa taaacctgac tgggcgcagt gataaattta atagatgttg
     5581 atgggggctt tcttaatata cgtctggata ctcgccattt cgtagccctg tttttgaaag
     5641 cgctctgtca taatgagagt tacccgatac cttattatca acaggtaact ctcaatcttt
     5701 aaaaaacgtc tgtattttta aatgtgataa actttatacc ttgagtacat ggagagaata
     5761 atcgcttatc agacttcagg tacctcaaag agtgtcttct taccgtgtct tttctgatac
     5821 tcattacagg tgttaatcat tttttttgcc gcttctttta gcaaagaaag cattaaatca
     5881 tcataatcaa cgtagaatct ggttgtttca gtttccgttt ttatctcccc gatggcggct
     5941 atttcggtgg ttaatttcaa tttggcggca tcgatatctt tattcgcttg tttaaccgtg
     6001 ttagacaggg tggtaaagaa actctgcaca gattttaact tgttcttcaa ttctggaatc
     6061 agtttccctt caactacgcc cgcagcaata gaataggaaa tgattaaacc aaatggaccg
     6121 gcgacgacac cggctgcggc accggcatac gcttccttcc tgattttatc tacctgtgac
     6181 tggaaatagc tgcttttttc tgaaaaatca ttggttaact ggctatctaa cgccagcagt
     6241 ttcccggaag cgttgttgaa actttgtgag cttaccagca gggatttttg cgcttcattc
     6301 agcttcgtga taccgtcatc cagtacctta atgagaatgt ctttctgggc ggatgctttc
     6361 ttctcattgt actcatcaaa tagcaaaata tacgctgcga gcaattgcgt cgcaacacca
     6421 caccattcat acactgtttg ggttgcttca aaatacttat cctggctatc cataagtaag
     6481 gttttaatat cgccaactaa aacggaggct gcctgtgaat actcctgttt aaagcgactt
     6541 aactctttta tggtttcatc aaaggtctgc caggggatga cctgatcgag atatttatta
     6601 taaagatcta atgctccatc tgcggtttcg attgcgtttt taactacctc taccgtttta
     6661 tctgcaacga tttcagtcat aatcattcgc ctctttaaat atataaattg taatgaaact
     6721 cctgttttac aactattaaa gaattttaca tcatctaatt catagttagc cgggcgggat
     6781 gcgtcaatgt ctttatttct attaatatga taaatatcaa acaatgttta atgtcattat
     6841 ggctgatgct tctattctat tttttagccg ggtgatattt ttcatttctg ctggatgagc
     6901 gtcgtcgcca gaaggcgcgt gagcacagga taagataacg aaaaatcagc agtctatgca
     6961 gcgacaaata ttgatagcct gaatcagtat tgatctgctg gtaagaacag actactgtat
     7021 ataaaaacag tataacttca ggcagattat tatgttgttt atcaagcctg cggatctccg
     7081 cgaaattgtg acttttccgc tatttagcga tcttgttcag tgtggctttc cttcaccggc
     7141 cgcagattac attgaacagc gcatcgatct gaatcaactg ttgatccagc atcccagcgc
     7201 gacttacttc gtcaaagcaa gtggtgattc tatgattgat ggtggaatta gtgacggtga
     7261 tttactgatt gtcgatagcg ctattaccgc cagccatggt gatattgtca tcgctgctgt
     7321 tgacggcgag tttacggtga aaaaattgca actacgcccg actgtacagc ttattcccat
     7381 gaacagtgcg tactcgccca ttaccatcag tagcgaagat acgctggatg tctttggtgt
     7441 ggtgatccac gtcgttaagg cgatgcgctg atgtttgccc tctgtgatgt aaacgcgttt
     7501 tatgccagct gtgagacggt gtttcgccct gatttatggg gtaaaccagt ggttgtgcta
     7561 tcgaataatg acggttgcgt tatcgcccga aacgctgagg caaaggcgct gggtgttaaa
     7621 atgggcgatc cctggttcaa acaaaaagat ctgtttcgtc gctgtggcgt ggtttgcttt
     7681 agcagcaatt atgagcttta cgcagacatg agcaatcggg tgatgtcgac gctggaagaa
     7741 ctatcgcccc gcgtcgagat ttacagtatt gatgaggcat tctgcgatct gaccggtgtg
     7801 cgtaattgtc gcgatctgac tgattttggc agagaaattc gcgcaacggt gctacaacgt
     7861 acccatctta ctgttggtgt ggggatcgcc cagaccaaaa cgttggctaa gcttgccaat
     7921 catgcggcaa aaaaatggca gcggcagacg ggtggggtgg tggatttatc aaatctggaa
     7981 cgccagcgta aattaatgtc tgctctcccc gtggatgaag tctgggggat tggacggcgg
     8041 atcagcaaaa aactggatgc gatggggatc aaaaccgttc tcgatttggc ggatacagat
     8101 atccggttta tccgtaaaca ttttaatgtc gtgctcgaaa gaacggtgcg tgaactgcgc
     8161 ggcgaaccct gtttgcaact ggaagagttt gcaccgacga agcaggaaat tatctgttcc
     8221 cgttcgtttg gtgaacgcat cacggattat acgtcgatgc ggcaggccat ttgtagttac
     8281 gctgcccggg cggcggaaaa acttcgcagc gagcatcaat attgtcggtt tatctccacg
     8341 tttattaaga cgtcaccatt tgcgctcaat gaaccttatt acggcaatag cgcgtcggta
     8401 aaactgctga cgcccactca ggacagcagg gatatcatta acgccgctac gcgatctctg
     8461 gatgccatct ggcaagcggg ccatcgttat caaaaagcgg gcgtgatgct gggggatttc
     8521 ttcagtcagg gagtcgcgca gctcaattta ttcgatgaca acgcaccgcg ccccgggagt
     8581 gagcaattga tggcggtaat ggatacgcta aatgctaaag agggcagagg aacactctat
     8641 tttgccgggc aggggatcca gcaacaatgg cagatgaagc gtgccatgct ttcaccacgt
     8701 tatacaacgc gaagttctga tttactgcgg gtcaaataaa catagcggca ggaaaaaagc
     8761 gctcccgcag gagcgctgaa tggattagcg accgaacagg tcacgttttt tcgctttaaa
     8821 cggctgggaa atcaccacca gcaccgcgac aatcaggtaa gcgataaaaa taccgagcaa
     8881 ccactgcggc atttccagac ctaaaaaatc ccactgacgc tcggcacaat cgccagaggc
     8941 gacaaacact tgcggcaccc atttatccag cggcagccat tccgggaaac gaaccataaa
     9001 atcacaggtg gcaaacggcg aaggatagag ctgaagcatg gtgtgctcgt aagttaactg
     9061 cacaccgcgg aacgcactat acaaccagat aaccatcgct acataacgca gtggcgtttt
     9121 tggggcgatc gcgccaatca gcgcagcacc cagaacgccg aataatgcgc agcgttcata
     9181 aatacagagc acgcaaggtt tcagtaacat cacatgctgg aaccacagcg ccgtcagttc
     9241 cagtgccaga gcagtaaacg ccatcaacag ccacgcgccc cggccttgtg aacattggtt
     9301 caaaaatcgc aacataatca tttccctgca atatgcatag agtgcgcagt ttaaaccaat
     9361 tcattcgctg cgccaccagg aggcagcaaa aataggacgc aattggacaa ttatcgggcg
     9421 aaaaggcaaa ccgggcattt atatgcccgg taagttgtta tcaaagcgtt gctatccagc
     9481 ccatttgcat aaaccattcg gttacagggg caagcgtaaa ctcgacgcag agcagtccga
     9541 cgagtgtcag tacgagggtg taaggcaggg ccatccacac catgcggcca taagagaggc
     9601 gaatcaatgg cgcgagtgca gaggtcagca ggaacaggaa cgcagcctga ccgttcggtg
     9661 tagcgacaga gggcagattg gtaccggtat taatggcgac cgccagcagc tcgtattgct
     9721 tcaacgtgat agcgccactt tccattgccg ctttcgcttc gttgatataa atcgtcccca
     9781 cgaagacatt atccgaaatg gatgacagca ggccgttaaa aatatagaac aacgacagct
     9841 gggcgtgttc cgatgcctgt aggacaaaat gaatgattgg tgagaacagt tgctgatcaa
     9901 taattaccgc gacgaccgag aaaaagacag tcaacagtgc ggtgaatggc agagattcgg
     9961 tgaaggcttt accgatagca tgttcatcag tgacaccagt caatgatgtc gccagaataa
    10021 tgaccgacaa accaatcagg ccaacctccg ccagatgcaa cgccagcgcg gtgaccagcc
    10081 agacgccgat aatcgcctgg acaatcagac gaattttatc ctgacgggta cgctggtggc
    10141 gactttgatc gtcaaactgt tgcagcactt cgcggacttt ctccggcagc gtttcgccgt
    10201 agccaaaaca acgcagcttc tccaccagca ggcaggtcaa caggccacaa atcagaaccg
    10261 gaacggtcac tggcgacatg cgcaggaaga aatcgccaaa atgccagcca gctgctttag
    10321 cgatgatcaa gttctgtggt tcgcccacca tggtcatcac gccgcctaat gcggtgccaa
    10381 caccggcatg catcatcagg ctgcgcaaaa agccacggaa ctgttccaga accactttgt
    10441 aatgcttgtc gatatgacta tcgtcttgca ggtcggtatc ttcagtacgg gaagaggcaa
    10501 cacgatgata aataccataa aaaccgactg cgacgctgat caccaccgcc acgacggtta
    10561 aggcatcgag gaacgcggag aggaacgccg ccgccatgca aaaagagagc gacagcagca
    10621 ttttggagcg aatgcttagc agcaaacggg taaatatgaa cagcaacagc tgtttcataa
    10681 aatagatacc cgccaccata aacatcagta acagcaagac ttcaagattc gccgccacct
    10741 cttcacgaac atgttccgcg ctggtcatgc cgatgaatac cgcttcgata gccaacaggc
    10801 caccggggag tagcgggtag catttgaggg ccatcgccag agtgaaaata aattccgcca
    10861 ccagcagcca accggcaaca aaggggctga tgatgaaaat taacggatta agaattaaga
    10921 aaataatgag ggcgagtttg taccagtcgg gggactggcc caaaaagttg cgccataggg
    10981 cacggcccca ggagatctcc atgatggttt cccttacctt acaaataatc aatgatgttt
    11041 ttatcttaga acgcaaagct taacggtcag gaggggatcg aggcaagtgt tgatggtctg
    11101 gagaaaagag atgtagaaaa tgaagccttg atcccttttt tcttcttttt gtgtgctatc
    11161 agcgtagata gccctctggt atgatgagtc caactttgtt ttgctgtgtt atggaaatct
    11221 cactatggtc attaaggcgc aaagcccggc gggtttcgcg gaagagtaca ttattgaaag
    11281 tatctggaat aaccgcttcc ctcccgggac tattttgccc gcagaacgtg aactttcaga
    11341 attaattggc gtaacgcgta ctacgttacg tgaagtgtta cagcgtctgg cacgagatgg
    11401 ctggttgacc attcaacatg gcaagccgac gaaggtgaat aatttctggg aaacctccgg
    11461 tttaaatatc cttgaaacgc tggcgcgact ggatcacgaa agtgtgccgc agcttattga
    11521 taatttgctg tcggtgcgta ccaatatttc cactattttt attcgcaccg cgtttcgtca
    11581 gcatcccgat aaagcgcagg aagtgctggc taccgctaat gaagtggccg atcacgccga
    11641 tgcctttgcc gagctggatt acaacatatt ccgcggtctg gcgtttgctt ccggcaaccc
    11701 gatttacggt ctgatcctta acgggatgaa agggctgtat acgcgcattg gtcgtcacta
    11761 tttcgccaat ccggaagcgc gcagtctggc gctgggcttc taccacaaac tgtcggcgtt
    11821 gtgcagtgaa ggcgcacacg atcaggtata cgaaacagtg cgtcgctatg ggcatgagag
    11881 cggcgagatt tggcaccgga tgcagaaaaa tctgccgggt gatttagcca ttcaggggcg
    11941 ataatccctt ccgtttaaaa agcaaacccc tcaaacgagg ggttttttgt tgtttttaca
    12001 gatttcccat tctcggcggg caacgttcca gcaactcgac gctgccgtct tcgttttgct
    12061 gttccagcat cacatcaaat ccccacaggc gatgcacatg cttcagtact tctttgcgcc
    12121 cccgatccag cggtgcgcga ttatgtggaa tgtaacgcag cgtcagcgaa cggtcgccgc
    12181 gcaaatccac gttccagatc tgaatattcg gctccagatt acttaaatta tattgcgacg
    12241 ataaccggtt acggatctcc cgataacctt cttcattatg aatagcggaa atctccagat
    12301 aattatgccg atcgtcgtcc agcacggtga agaagcggaa atcacgcatc actttcggtg
    12361 acaggaactg actgataaag ctctcatctt tgaaatcacg catcgcaaaa tgtaatgtgt
    12421 ccagccagtc agaaccggcg atatccggga accagtattt gtcttcttcc gttggcgact
    12481 gacaaatccg tttaatatcc tggaacatgg cgaacccgag ggcatacggg ttgatgccgc
    12541 tgtaccacgg gctgttatag ggcggctgga agaccacatt ggtgtggctg tgcaaaaact
    12601 ccagcataaa acgttccgtt actttccctt catcatacag atggttaagg atggtgtagt
    12661 gccagaaggt cgcccagcct tcgttcatca cctgagtctg tttttgcgga taaaaatact
    12721 ggctcacctt acgcacaata cgcagaattt cacgctgcca tgattccagc agtggcgcat
    12781 ttttctccat aaaatagagc aggttttctt gtggctcgga cggatagcgg cgcgcttcag
    12841 caaccgtttt ctcttcctcg cgcttcggca gggtacgcca gagcatattg acctgacttt
    12901 gcaagtactc ttcgcggctt ttctgccggg ctttctcctc ttgcagcgag attttttgtg
    12961 ggcgtttata ccggtccacg ccgtagttca tcagcgcatg gcacgagtcc agcagccgtt
    13021 ctacttcatc cacaccatag cgctcttcgc actcggtaat atatttacgg gcaaaaatca
    13081 gataatcgac aatcgaactg gcgtcggtcc agctacggaa taagtaattg tttttgaaga
    13141 aagagttatg cccatagcag gcgtgtgcca tcaccagcgc ttgcatggta atggtgtttt
    13201 cttccatcag gtaagcgata cacgggttag agttaatgac gatttcatag gccagtcctt
    13261 gctgaccgtg cttatacagc cgttcagtct cgataaactt tttaccgaat gaccagtgcg
    13321 gatagttaat tggcatgccg acgctggagt aggcatccat catctgttct gaggttatca
    13381 cttcaatctg gtgcgggtag gtatccagcc ggtagagttt cgccacccgg tctatctctg
    13441 ccagataaac atccagcagg tcgaacgtcc agtcgggtcc atcgctcaaa cgtgtggtgt
    13501 ccttattcat agaatcgatc gtcgccatac gcgcacctca ttgttgtcgg cgctctctgt
    13561 gtggagcacc tcatttcaag catagaacac ctgttaaaaa ccgcgtcgcc ggagaatttt
    13621 tttctttgcg atttcttatt atcagagtac cactaatccg cttctgaacg gaattttatg
    13681 ctggataaaa agggcgttca gcaggagata ctaaagacgc catattgccg cagagtcagg
    13741 gagatgtgag ccagctcacc ataaaaaagc cgtatgttga ataatatttt caactgagtt
    13801 atcaagatgt aattagatta ttattctttt actgtatcta ccgttatcgg agtggctatg
    13861 cgagttgtca tactgggaag tggtgtggta ggcgttgcca gcgcctggta cttaaatcag
    13921 gcaggacatg aggtcaccgt cattgatcgg gagccggggg ccgccctgga aaccagtgcc
    13981 gcgaatgccg ggcaaatctc ccccggatat gcagcaccgt gggcggcacc aggtgtgcct
    14041 ttaaaagcga ttaaatggat gttccagcgc catgcgccgc tggcggttcg tctcgacggt
    14101 acgcagttcc agttgaaatg gatgtggcaa atgttacgta actgcgacac cagccactac
    14161 atggaaaaca aagggcggat ggtgcgtctg gcggaataca gccgtgattg cctgaaagca
    14221 ttgcgcgccg aaaccaatat tcagtatgaa gggcgtcagg gtgggacgct gcaactgttc
    14281 cgtaccgaac aacagtatga aaatgcgacc cgcgatatcg ccgtgctgga agatgccggc
    14341 gtaccgtacc agctgctgga atccagccgt ctggcggaag tggagcccgc gctggcagaa
    14401 gtggcgcaca aactgacggg ggggctccag ctgcccaatg atgaaaccgg agactgccag
    14461 ctgtttaccc agaatctggc gcggatggcg gagcaggcgg gggttaaatt ccgctttaat
    14521 acgcccgttg accaactact ttgcgacggc gagcaaatct acggcgtgaa gtgtggcgat
    14581 gaagtgatta aggccgatgc atatgtgatg gcatttggtt cttactcgac ggcgatgctc
    14641 aaaggcattg ttgatattcc ggtgtatccg ctgaaaggct actcgctgac tattccgatt
    14701 gcacaggaag atggtgcgcc ggtgtctacc atccttgatg aaacctacaa aatcgccatt
    14761 acccgtttcg ataaccgcat tcgtgttggt ggaatggcgg aaattgttgg ctttaatacc
    14821 gagctgttgc aaccgcgtcg tgaaacgctg gagatggtgg ttcgcgatct ctatccacgc
    14881 ggcggtcatg tcgagcaggc gactttctgg actggtctgc gcccgatgac gccagacggc
    14941 acgccggttg tcgggcgtac acgctttaaa aatctgtggc tgaataccgg tcacggcacg
    15001 ctcggctgga cgatggcttg cggttccggt cagttgttaa gcgatctgct ctctggtcgc
    15061 acgccagcga tcccgtatga agatctaagc gtagcgcgct acagccgtgg atttacgcca
    15121 tcacgcccgg gccatttaca tggcgcacac agctaaggaa acgagatgac ccgtccgata
    15181 caggccagcc tcgatctgca ggcattaaaa cagaatctgt ccattgtccg ccaggccgcg
    15241 ccgcgcgcgc gcgtctggtc ggtggtaaaa gcgaacgctt acgggcacgg tattgagcgt
    15301 atctggagcg cgctcggggc caccgatggc tttgcattac ttaacctgga agaggcaata
    15361 acgttacgtg agcgcggctg gaaaggaccg atcctgatgc tggaaggatt tttccatgct
    15421 caggatctgg agatttatga ccagcaccgc ctgaccacct gcgtacacag caactggcag
    15481 ctcaaagcac tgcaaaatgc acggctaaaa gcaccgttgg atatttatct taaagtgaac
    15541 agtgggatga atcggttggg cttccagccc gatcgcgtgc ttaccgtctg gcagcagttg
    15601 cgggcgatgg cgaatgttgg cgaaatgacc ctgatgtcgc attttgccga ggcggaacat
    15661 cctgatggaa tttccagcgc gatggcgcgt attgagcagg cggcggaagg gctggagtgt
    15721 cggcgttcgt tgtccaattc ggcggcgact ctgtggcacc cggaagcgca ttttgactgg
    15781 gttcggcctg gcattatttt gtatggcgct tcgccgtccg gtcagtggcg tgatatcgcc
    15841 aataccggat tacgcccggt gatgacgcta agcagtgaga ttattggtgt ccagacgcta
    15901 aaagcgggcg agcgtgtggg ctacggcggt cgctatactg cgcgcgatga acagcgaatc
    15961 ggcattgtcg ccgcaggata cgccgacggt tatccgcgcc atgcgcctac cggtactcct
    16021 gttttagtgg acggcgtgcg caccatgacg gtggggaccg tctcgatgga tatgctggcg
    16081 gttgatttaa cgccttgccc gcaggccggt attggtacgc cggttgagct gtggggcaag
    16141 gagatcaaga ttgatgatgt cgccgccgct gccggaacgg tgggctatga gttgatgtgc
    16201 gcgctggcgt tacgcgtccc ggttgtgacg gtgtaacttg ttgtaagccg gatcggaggc
    16261 aacgtcttct ggttgcaaaa aaaaatcatc catccggctg gtcagcaact gtagttgtta
    16321 atgtgacaga gccattgccc atgatagtgt ccattaaaag aatggacact atttccccgg
    16381 aacctgaact caccgcacag gcgttctaca taaaacgctt acgcttcatt gttgactcgg
    16441 agtcgacttc agataaatca cgcttcacct ttgatgcaga ttcgggttca tgttcagatt
    16501 gttgattaac aggctgcgag tcctgttgtt tttccagact caacaggccc aactgttgat
    16561 ccaggagctc caggtcctcc tgtaccttca cgcgctccag gagttccggg tcatccagga
    16621 actccagatc cataaggtca tccaggtcct ccagctcctc gagtccttcc aggtcaacca
    16681 gaccttcctt gtgcaaaaag tccaaaagct cctccagacc ctcctggttt ttcaggccct
    16741 cgacggcttt gagcagcgtc ggtaaattgt ctttgtccca cgggttacca ttacagctaa
    16801 acaccttcag gcttcggggc tgcggcaccg gtacagtttt aaactggttt tctccacagt
    16861 agtatatgat tagtttgggc ggcagccgtg gtgaagagat caaatcgtta ctagaacagt
    16921 caaatacctt caggcgagtc aagttctcca gcatctcatc aggtattaag ctaatactgt
    16981 tgaaagcaca gcgcagtgta tacaggtact ggggcatcga cggcggtaaa aattgcaaga
    17041 agttttcaga acagtcaatc actctcaggt tagtcgacag ctcaggcagc cagataatat
    17101 agttttcata gcacttcagt accatcagtt tcgggggctg caccttcggt aacaagatca
    17161 aaaagttctc catacattca agcactctca gcccctttgg cattacctcc ggtaacgcgg
    17221 tcaacatgtt tctactgcag ttaaattcaa tcagatgtgg aggcaaattt tcagggagtg
    17281 aagtcagtcc aaggttgcta agatcgagca tactctgtct tgttatgaga cattccttca
    17341 ttctgcgcaa tgcttctgcc cggtcttcaa tctggcttgc tgactggttt tctttcagga
    17401 tatcttcctt gatttctttc agcctttctt tcaggtcttc ttggggaatt tctgactgtt
    17461 tgttttgccg gttttcttcc aggacctcgt gcagtttttt ttgcaggttt tcttgcaaac
    17521 catcctgctg tatagcccat tcgcaccatt tggcgaccat catgaactcg ttatgttgct
    17581 gattcagggg agtccccaac gtgatgatgg tattgttgtc cacctgtatg ttcactttgc
    17641 ccgcggtact aaggtcaatg atctgccagg gggcatcacc ccagctgaat ttttgcgggc
    17701 tgagatactt catgttctgc aaaatttgat tcttgagttc cgccgacagg aagagccgcc
    17761 ctggcgggcg tcgtacgcct gggttaacgg aagatggagg aatggcgggg gcgaatgggc
    17821 agatgtcttt aaagctagcc ataatgctac gtatccttga aataaatatc ctctggcagc
    17881 gccggagacg tgaatgtggt caccgcaaag gagccacagg acgtcatttg cgctgatata
    17941 actactcatc gttgactttt tccctggtca gaaatgccgt gtcgggtctg gtacatgctg
    18001 tattacggcc caggtagctc tgacgtatta aagaaaatac gagagcgaac gacccggaaa
    18061 taatcgttat taagcaaagg aataactata agaaatcgct catgcatccg gtgctacagg
    18121 cggatgaaac gcctattgtg tgggctacgt cagtgcggca ggttgcgtgg gggaatatgt
    18181 tcaggcgatg tcaggcaggg gcccctgatg gttgacgggg ataccggtaa tgcggggctg
    18241 gtttttcttc acctgttgca gtcggaagaa acccttacgc tgactttctt gagccggtgt
    18301 tttgagttca ttagccagag cctgcaatgg tttgtcatcc ataatttaac tttcttctga
    18361 tgttggaatg aacatatcaa aatcaggaaa tgacaagttt attacaggct caggcagcgg
    18421 tcagtaaagc atcgcatccg gcgatagatg ctgcattaaa tttaagattc agcttcctct
    18481 tcagccaccc gtacgccaat cttcaacact tcattatctt ctttctcggc caccgtccag
    18541 atcatcccgg caaactctac ctggtcaccg acaaccggtg ccgcgcctaa caactgctgg
    18601 acgatttcac caagcgtttg ctgcttatca cgatattctc gcccgtcttc caggccatat
    18661 atcagcgcca catcagcata tttggcgctg gcttcgagaa tgaagtcacc aaagaagcgt
    18721 tgatccagcg cgaccggtgg cgactggctg aacagtttac cgagcgccgg aagatcgcgt
    18781 tcccgaccaa ttacacacaa tacatcgcct tcgcgcagtc gggtgctgcc ggtaggatga
    18841 agcagctggt tatcacgaaa cagtgccgca atacgcgtct cttttggcat atgcaaatcg
    18901 cgcagtgccg cgcccacgca ccatttatcg gcactcaatt gataaacaaa ctgctcccac
    18961 ggattttccg gatgaatatc caggccaacg cgtgacaccg gacgtccgac gggcggaacc
    19021 accactttgg cttttttcgc cgcccacgag agtgatgttc cctgcaagag cagtgaaacc
    19081 agaaccacaa agaaggcgac attaaagaac agacgtgcat tctccagccc cgccatcatc
    19141 gggaacacgg ccagaataat cggcaccgcg ccgcgtaatc ccacccagct gataaacacg
    19201 cgctcacgca gattgaagcc acggaatggt agcaatccgg caaataccga aagaggacgg
    19261 gcgaagaaaa tcatccatgc ggacaaaatg agcgccggaa tggcaatagg tagcagatcg
    19321 cttgggttaa ccaacagccc cagcaccagg aacatggcga tttgcgccag ccaggcgagg
    19381 ccgtcgaaat tttgcagaat gccgtagcgg ttgcgaatcg ggcgattacc cagcagaaaa
    19441 ctgcacagat aaacagccag aataccgctg ccttccagcg ccgtggttaa agcaaaaatt
    19501 aaaataccgc cgcttaatgc cagcaatgga tataatccgg cgggcagggc gatgcgatta
    19561 atcatttgca gcagtaaata accaccgcca agcccgatga caatcccgag gccaaattgc
    19621 tgcagaatat cgaccacgaa catccaactg acactgcttt catgttgctg gatcatcgca
    19681 atcagcgtaa tcgtcagaaa gaccgccatt ggatcattac tgccggattc aatttccagc
    19741 gtcgagccaa cacgttcgtt aagcccctta ccacccagca gagaaaagac cgctgcagca
    19801 tcggtggaac cgacgatagc gccgattaat aatccttcaa tcaaatcaag attaaacagc
    19861 cacgccgcca tcatgccggt taaacctgag gtgataagta cgcccagcgt cgccagcgac
    19921 agtgccggtc ctaacgccac acgaaaggaa ctggcctgag tgcgcattcc gccgtcgagc
    19981 aaaataatcg ccagcgccag gttacttacc atgtaggcga aggggtaatt atcaaacggg
    20041 ataccgccga cgccatcgac tcccgccagc atgccgatcg ccagaaaaat aaccagaata
    20101 ggaatgccaa gacgggaaga aaatgaacta agtaatatac tgctggtgac aaggatggat
    20161 cctaagatga aaaggctaat tattgttgtg gcatccaacg gtcggttact cctgattacg
    20221 ctgtctctta tataaaccct accatattag cggcagagac agcgttttac ttagccctga
    20281 agcgtgattt ttttacattt taagaacagg atgaccgtta atcgtcaact gagtgccttc
    20341 gtgggtgtta ttaagaatgg catgtgcgcc caacggcagc gtaaccgtac gttgttcatg
    20401 accaaaatcg aggccggtaa tgagtggagt cgacaggcgg gaacgcaaaa acgcgtacac
    20461 tgactccagg ttgtaacctg cgtcataatc attgggcgtg ctgccgctaa agctaccgag
    20521 aataatcgcc ttctgacgtg gcaaaattcc agcatgatag agctgcaaca gcatacgttc
    20581 gacgcggaaa ggatgctcgt taatatcttc cagcaccaga ataccgtttt cgatttgcgg
    20641 catccacggt gtaccaatca gtgaaatcag catcgcgaga ttgcctcccc ataacgtgcc
    20701 ttcggtctga catgtcgggc cttccccttg ccattcaatg gtgaaggttt cattgcgtaa
    20761 cgccagccag aaatggtgct cggtaaaggc gttcagttca tccgcgccaa agtttgctac
    20821 cagcatgggg ccgctaaagg tgatgacatt gccatgcgcc agaagaccgc actgaatggc
    20881 ggtaaaatcg ctatgtccgc aaatgagcaa cgggtcatgt tgttggcgag ccactagtgc
    20941 ctgccagtca atatctgcca gtaaacgact ggcaccgtaa ccgccgcgta cagccagcac
    21001 gatggtgttg ggggtggtca gtctggcaag ggaattgaga tcttccagac gctccgtttc
    21061 cgtacccgca aaacgctcac aacgacgggc aataacctcg acgttattga cctgatgccc
    21121 cgcgtcggta aggcgctgga taccacgcag cgcggcgtgc tgtttaatgc agtaacccga
    21181 tggggcaatt aagtgaaaca gagacatggc aattccttgc tgacaacaga aacgaaatgt
    21241 atatcatgcc gcttaggtgt gccgttgtca cctcaacggc gattccaggc tataaggata
    21301 gaagaagtga aattgagatg gtttgccttt ttgattgtgt tattagcggg ttgttcatca
    21361 aagcatgact atacgaaccc gccgtggaac gcgaaagttc cggtgcaacg tgcgatgcag
    21421 tggatgccaa taagccagaa agccggtgca gcctggggcg tcgatccaca attgatcacg
    21481 gcgattatcg ctatcgaatc gggtggtaat ccgaacgcgg tgagtaaatc gaatgccatt
    21541 ggtttgatgc agataaaagc ttcaacctcc ggacgtgatg tatatcgtcg tatgggctgg
    21601 agtggtgagc cgacgaccag cgagctgaaa aatccggagc gtaatatttc aatgggagcg
    21661 gcttacctga atattctgga aaccggcccg ctggcaggca ttgaagatcc gaaggtactg
    21721 caatatgcgc tggtggtgtc atatgctaac ggggcaggtg cgctgctacg gactttctcg
    21781 tcagatcgga aaaaggcgat cagcaaaatc aacgatctgg atgctgacga gttcctcgat
    21841 cacgtagcgc gaaatcatcc tgcgccgcag gctccgcgct atatctacaa acttgagcag
    21901 gcactggacg cgatgtaaat cagtcgcgca ctttgtccgc ttttttccgg gcttctcgct
    21961 cgagagagaa aataatccgc tgtaattgcc gctccaccgt cgggctgacg ttaagaaaac
    22021 ggaagctcag acggggagtg gtgatggttt cattcttgcc atcaatcact ttgcgctcgc
    22081 tgatggagat taactgggcg tcaaagtgaa aaacacccca ttgccccatg ttgacttcaa
    22141 tctgagcgaa gcgcatgcct tcgtgcaatc cggcaggctt tgctgtttcc agtaatgcgc
    22201 ccatgccgcc taacgataaa tcatacaggc ggaaacgtaa cgtactgtta tccgccagtt
    22261 tggtctggca aaaataaggc ggatggagtg gggcggagat gcggaaatat cggcgtcgtt
    22321 gtacaaacca caaggtggga ggcggtacgg taataaatgc cggaagctgc aagtattcac
    22381 tctgctgtag ttgttcaaca gtaaactcga ctttcgcacc ctgagtttcg gcggtaatgg
    22441 taatgtgctg cgcctttagc acggcgatgt tgtcttcggc ctggctgccg aaatccagca
    22501 ccagtttatc cggagttatt gccagtattt tgctgatcag ctgcccgcca ttccagctaa
    22561 tacgcaaagg aattgcggct ttgtgcaaat cgcgtaacac gcccaggacg gctaacggat
    22621 tttgtttcag gaactgctca tggtaatgac tcacgcggaa aaactcctga ctgacaaact
    22681 gtcttaggtt tatcggtcac agttaacaaa acttaataca aatgcgtgaa tattttttac
    22741 atgtcgttct taaatcagcc gacatacgcc cagcatcgat cccgcccttg cctatactta
    22801 gagcgttgac gtaagcattt cttgcgtccg attcatcgaa acgagggcct gaacatggga
    22861 attattgcct ggattatttt tggcctgata gccggcataa tcgccaagct aatcatgccg
    22921 ggacgtgatg gtggtggatt tttcctgacc tgtattctcg ggatagtcgg tgcggtggtc
    22981 ggcggctggc tggcgaccat gtttggcatt ggcggctcca tcagtggttt taatctgcac
    23041 agcttcctgg tggcggtagt gggcgctatc ctggtgctgg gcgtattccg cctcctgcga
    23101 agagaataag attttcataa ggcggatagc gatacagatg ccgctatccg ctttcacatc
    23161 agaacgtata cgcgatacct gcccagaaat tgcgaccttc ttcaataaac ccttcactgt
    23221 aggcatattt ggtatcaaac aggttattga ccgacgcatt gacgctgaag ccatgaccta
    23281 aggtgtaatc ggctcgaatg tgggtcaccg caaaaccggc ggctttttgt gaaccgtcac
    23341 tgttgctgta gctggaggaa cgcgcctctt ccgacagcgt tacgcttaac ggctcccacg
    23401 gtttgagagt catccatgcg gtcattgtct gcgttggcag atcggttatc ttgccgatgt
    23461 ctttgcgttt ggcgtcagcg tggatcaggg cgtagctcaa tcctacatcc agtatattgc
    23521 tgattttccc cttaataccg gcatccagac cgctgtaatc caccgtgccg ctgttctgat
    23581 tttgaatggt atcggcatcg atattgtgcg agaggatggc atcactcacc cggttatagt
    23641 aaacgctgac ctcaaacccc cagtcgtgcg tgaaggcacc attccaggtt aaatccaccc
    23701 cgcgcgcgcg ttccggtttg agctgcgggt taacaatcgc tatctggtta tacgcaggtt
    23761 tggacgtggt atagcgttct ttcagcgtcg gaaagcgtgt gcggtcatag tacgaaagcg
    23821 ccagcgtgtc ttcattggca aagtggtatt tccccatcac ctgccagtta aaagctgact
    23881 gattgttgtc gtcataatgg gtgatactgc catctttctc atgttttttc gcttctacgc
    23941 tatcgcgcca gtcatagctg attccagcca cgacatcgac attatcggca gcagcccatt
    24001 gatattcact ggcgagcgac caggtacgat cttcatagcg atcgtaagcg gcgtgtggcg
    24061 cacctttttc ccggtgtacg tcatctttcc agttaacggc aaacgacagc agatcgtttt
    24121 cacgcacatc ggctgccagt tgtaacccgg caccgtcgct gtaatcggaa taatggctgt
    24181 agctgccttt tttatttttc aaatcagcca gcgagttgta catcattagc gtattttcaa
    24241 aggtgtcgcg atacagccga cttttcaggg taaaacgatc gtttagttgg gtcgttccct
    24301 gataataaaa actttctttg tcatactctg gccactgcca gtagcgtgat ttttgaccac
    24361 tatttccact gtatggcggg ttatcttttt caccatcctg cttaatgtaa gtcaatgtgt
    24421 attcatcgtt ttcgcgtggt gtaaaaccga gcttcacaat gccgcgttta tcatcagccg
    24481 atgaattaat catcttgccg tgtttgcctg caatatcatt atttacacca tgcggcaggc
    24541 cgagaaaatc ctgctttagc tggctaccgc tgacttgcaa ataccccaga tcgctgctgg
    24601 cggcaaatga agcatgcata tcgtaggcat tgtcctggct acggctccat ccctggcgat
    24661 atcccagatt tgcttccaga ggttttgttg gcttctgggt ggtgatatta atggctccgc
    24721 ccatctgatt aggcccctga agcagcgacg aatacccttt ggaaacttca actgccccca
    24781 aattgttggt cagaatccgc gccagatcga ggttgccgtc atagggaaca taaatgggca
    24841 caccgtcgaa atagactggc acctgacgac tatcaaagcc gcgaactttg acctgttctt
    24901 cgttgcggct acctgacttt tgcagcacca cgccggggac gacacttaat gcctgagcga
    24961 cattctgttt atccagggcc ttcatggtgg gttgatcgag aacggtggtc gtcgtcgatg
    25021 ataccggact ggaccatacg gtcagcgtgt cactctcctg agccgctacg gcctgctggg
    25081 taaaggcaag tgataatgct gtgcagagat aacgtttttt gagtctcata gaataaattc
    25141 cgtttaacaa agaattaaat agagactgcg ggttgcagac tgactttaat gtcagctggt
    25201 gtcgcgtaat aaggcgcgga ggtaataaaa aggcgaatgc cgcagtcgag gtaatttttg
    25261 agtgttgtca gattaattcc gccggtcaga gccagcgtgc agtgaggagc cagcgacggt
    25321 gctatttgcg caatttcagt tgcctgttgc ggattaaatt tgtcgagctg aagtacgtct
    25381 ggttgcgcgc gtaacgcggc gattgcctct ttcggcgtgt cggcttcaac aactattttc
    25441 ttctcgggtg cgtggcgacg taactgattg attgcgcctg accagtcctg attgtcatga
    25501 agaaaatgac ggtggttggc aaacaataat atggtttccg cacatccggc acgatgaatc
    25561 agtcctccgg cagccagaat tgcctgcgag gccagtaagc gggtgcccgg aattgctttt
    25621 cgggtgcagg cgatattgcc atcagggtaa cgttcacgaa gtaacgccag catttgagcg
    25681 agataatcag aaacaccgca actccactcc agcacattct ggactgcctt ccatccctga
    25741 tgaagtgctg cggcattacc ttgcgcacgg attagccgct gacccgcgtt cgcttgtgaa
    25801 ccgtcgctga ccgcgttatc aatagttaat cccagcgtag ttaacatctt acacgcgaca
    25861 gaaattccgc tgacgcagcc gccctgacgg tgaaaaaact ctatatagcc atgctggtgt
    25921 ccaatattta atgctcgcgt ggtcaggtca ccgccctgga tatcttccag aagtaacgca
    25981 tcgatttgtg cctgagaaag gaaaatcatc gcagtgcctc ttgcggtacg ctatcccacc
    26041 agatgagcgc ccagtcgcgg ctggcagggg cgagggcacg agcatcttgt tgttgatacc
    26101 agtgatataa ccgctcacgc tcatcgtcgt taagcgcgcc aagactccag ctgacgttct
    26161 gctcaaaacg ttcccaggtg ctgttatcct gttggcaatt ttgtccacgg ataaaatcaa
    26221 cgtgggcata aattcccatc tgatacagga cgttgagcgc aaatatatag ttgggtaatt
    26281 cgatcacttc ccgaccagcc gcccgctgaa tggctggaga gacgaacgag gtgctgacca
    26341 gatgagtcgt atagacgcga agccgtgcct gattattcag tttgctcatt gcctggcgca
    26401 tatcagccac cagagtggag cgggaagcca cggcaatatc gcagcgtggc aggtcactcc
    26461 agtcctcttc ccaggcgcgc tggatccagt ggacattgtc tgcctttagc gcggcggcgc
    26521 gacgggcggc aacgttcagc atcccctgac tgtaatcaac gccataaatc gtcgtgagtt
    26581 tgtcagccag cgccagcgat acggtacccg gtccgcagcc catatcgaat aaggtttgtg
    26641 cgccttgcag atcaatttta gcggtcagtt gttgcaggta gctgtccgta ggcgaggcgc
    26701 aattttccgc cattttttcc gcccgttgat cccagtgatc cggcgttttt tctgtgcgat
    26761 gggccagcct gagttgctga aggtaaaggt ctgcaaaatc aatatcatca atgagcatga
    26821 tttttcctta gtgcttaatt gcagagaggt gatggtgaat ctggtcggca gaaacccggt
    26881 acagggcagc cagttttttc gttgttaact gttcggtcgg taagccttgt gttacacgtc
    26941 catctggctc tacctggata atgctgtcgg caatggcgtt ggcatggagc ggatgatgag
    27001 tcgacattaa cattgtcatg ccgttatttt tgagctgcgc cagcgtgtcc agtagttgga
    27061 tctgatgacc gaaatcgagg ctggaggcag gctcatccag taataacaag cgcggttgct
    27121 ggacgagtgc acgggcgatt aaagccagct gacgttcacc gccgctaagc gtattccagc
    27181 gtcgtgtcgc cagatgcaaa atgcccagct tttccagttg ctccgttgct ttcaggcgtt
    27241 cttgcttacc gggaacggaa aaagcgccta tggtgggggc caggcccatc aacaccatat
    27301 ccagcacact aaaagcaaaa atgccgtcgt gcgcctgcgg cacccaggca atagcctgcg
    27361 cgcgctggcg atggctgagt gtctgcacag ggaggctatc cagtaagatc tcgcctttta
    27421 ataacggtat aacgccaaga atggagcgca tgagtgtggt tttgccgcaa ccattggctc
    27481 ctaacagaca acaaattgtt ccttgcgaca gggtgaagga cacgtcgcgt agcaccggat
    27541 ggcgggagta acccagagac gcatgacgga cggcaagcaa agtcactggc gacctccgcg
    27601 taacaacaaa aacaggaaaa acggtgcgcc aacaaaagcg gtcagaatgc caagtgggat
    27661 ttctgttgtt ccaatgctgc gtgccagcgt atcggtaagc aggagcagaa tcgcgccagt
    27721 acacattgcc atgggcaaca gctgctgatg gttatgaccg gtcaacaagc gggcaatgtg
    27781 cggaacgacc aggccgaccc aaccaattat gcccgctatt gctaccgtgc tggcggtgat
    27841 gagtgtggcg cagacaatca atccaaagcg cagacgtgtc acgttcagtc ccagcgaacg
    27901 ggcttcgtcg tcggaaagtg
//
LOCUS       DAMZFT010000059        27763 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.059, whole genome shotgun sequence.
ACCESSION   DAMZFT010000059 DAMZFT010000000
VERSION     DAMZFT010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 27763)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27763)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27763
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.059"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(<1..704)
                     /gene="wcaI"
                     /locus_tag="PN977_002610"
     CDS             complement(<1..704)
                     /gene="wcaI"
                     /locus_tag="PN977_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis fucosyltransferase
                     WcaI"
                     /protein_id="HDI6042629.1"
                     /translation="MKILVYGINYSPELTGIGKYTGEMVEWLAAEGHEVRVITAPPYY
                     PQWQVGENYSAWRYKREEGAATVWRCPLYVPKQPSTLKRLLHLGSFAASSFFPLMAQR
                     RWKPDRIIGVVPTLFCTPGMRLLVKLSGARTVLHIQDYEVDAMLGLGLAGKSKGGKVA
                     QLATAFERSGLHNVDNVSTISRSMMNKAIEKGVAAENVIFFPNWSEIARFQHVADADV
                     DALRNQLGLPDNKKIIL"
     gene            complement(701..1180)
                     /gene="gmm"
                     /locus_tag="PN977_002611"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
     CDS             complement(701..1180)
                     /gene="gmm"
                     /locus_tag="PN977_002611"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose mannosyl hydrolase"
                     /protein_id="HDI6042630.1"
                     /translation="MFLRQEDFATVVRSTPLVSLDFIVENSRGEFLLGKRTNRPAQGY
                     WFVPGGRVQKDETLEAAFERLTMAELGLRLPITAGQFYGVWQHFYDDNFSGTDFTTHY
                     VVLGFRFRVAEEELLLPDEQHDDYRWLTPDALLASDNVHANSRAYFLAEKRAGVPGL"
     gene            complement(1183..2148)
                     /gene="fcl"
                     /locus_tag="PN977_002612"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
     CDS             complement(1183..2148)
                     /gene="fcl"
                     /locus_tag="PN977_002612"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
                     /EC_number="1.1.1.271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HDI6042631.1"
                     /translation="MSKQRIFIAGHRGMVGSAIRRQLEQRGDVELVLRTRDELNLLDS
                     RAVHDFFASERIDQVYLAAAKVGGIVANNTYPADFIYQNMMIESNIIHAAHQNDVNKL
                     LFLGSSCIYPKLAKQPMAESELLQGTLEPTNEPYAIAKIAGIKLCESYNRQYGRDYRS
                     VMPTNLYGPHDNFHPSNSHVIPALLRRFHEATAQNAPDVVVWGSGTPMREFLHVDDMA
                     AASIHVMELAHEVWLENTQPMLSHINVGTGVDCTIRELAQTIAKVVGYKGRVVFDASK
                     PDGTPRKLLDVTRLHQLGWYHEISLEAGLASTYQWFLENQDRFRG"
     gene            complement(2151..3272)
                     /gene="gmd"
                     /locus_tag="PN977_002613"
     CDS             complement(2151..3272)
                     /gene="gmd"
                     /locus_tag="PN977_002613"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HDI6042632.1"
                     /translation="MSKVALITGVTGQDGSYLAEFLLEKGYEVHGIKRRASSFNTERV
                     DHIYQDPHTCNPKFHLHYGDLSDTSNLTRILREVQPDEVYNLGAMSHVAVSFESPEYT
                     ADVDAMGTLRLLEAIRFLGLEKKTRFYQASTSELYGLVQEIPQKETTPFYPRSPYAVA
                     KLYAYWITVNYRESYGMYACNGILFNHESPRRGETFVTRKITRAIANIAQGLESCLYL
                     GNMDSLRDWGHAKDYVKMQWMMLQQEQPEDFVIATGVQYSVRQFVEMAAAQLGIKLRF
                     EGTGVEEKGIVVSVTGHDAPGVKPGDVIIAVDPRYFRPAEVETLLGDPTKAHEKLGWK
                     PEITLREMVSEMVANDLEAAKKHSLLKSHGYDVAIALES"
     gene            complement(3299..3847)
                     /gene="wcaF"
                     /locus_tag="PN977_002614"
     CDS             complement(3299..3847)
                     /gene="wcaF"
                     /locus_tag="PN977_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaF"
                     /protein_id="HDI6042633.1"
                     /translation="MQDLSGFSVPKGFRGGNAIKVQLWWAVQATLFAWSPQVLYRWRA
                     FLLRLFGAKIGKNVVIRPSVKITYPWKLTLGDYAWVGDDVNLYTLGEIIIGAHSVISQ
                     KSYLCTGSHDHASQHFTINATPIVIGEKCWLATDVFVAPGVTIGNGTVVGARSSVFKS
                     LPANVVCRGNPAVVIRKRVETE"
     gene            complement(3863..4609)
                     /gene="wcaE"
                     /locus_tag="PN977_002615"
     CDS             complement(3863..4609)
                     /gene="wcaE"
                     /locus_tag="PN977_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaE"
                     /protein_id="HDI6042634.1"
                     /translation="MLLSIITVAFRNLAGIVKTHASLAHLAQADDISFEWIVVDGGSN
                     DGTREYLENLKGIYNLRFVSEPDNGIYDAMNKGIAMAQGKFALFLNSGDIFHQDAANF
                     VRKLKVQKDNVMITGDALLDFGDGHKIKRSAKPGWYIYHSLPASHQAIFFPVSGLKKW
                     RYDLEYKVSSDYALAAKMYKAGYAFRKLNGLVSEFSMGGVSTTNNMELCADAKKVQRQ
                     ILHVPGFWAELSWHLRQRTTSKTKALYNKS"
     gene            complement(4620..5837)
                     /gene="wcaD"
                     /locus_tag="PN977_002616"
     CDS             complement(4620..5837)
                     /gene="wcaD"
                     /locus_tag="PN977_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative colanic acid polymerase WcaD"
                     /protein_id="HDI6042635.1"
                     /translation="MSTSIRICSYLLLPLIYLLVNVKIAQLGESFPITIVTFLPVLLL
                     LFLERINVKKLMIALGVGAGLTAFNYLFGQSLDASKYVTSTMLFVYIVIIIGMVWSIR
                     FKTISPHNHRKILRFFYLVVGLVVALAAVEMAQIILTGGSNIMESISKYLIYSNSYVL
                     NFIKFGGKRTTALYFEPAFFALALISIWLSIKQFGIKTPKTDAMILAGIILSGSFSGV
                     MTFILFYLLEWAFQYLNKEAIKKKLPLALVSLAVFLVGVVIAFPYISTRLGDLGTEGS
                     SSYYRIVGPLVMVGYSLTHIDGVVRFGSLYEYVASFGIFNGVDVGKTIDNGLYLLIIY
                     FSWFAVFLSLWYMGKVIKMMINAFGDNRNFRVQLYLFTPVSLFFTGSIFSPEYAFLIV
                     CPFILRKALNITR"
     gene            complement(5812..7029)
                     /gene="wcaC"
                     /locus_tag="PN977_002617"
     CDS             complement(5812..7029)
                     /gene="wcaC"
                     /locus_tag="PN977_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaC"
                     /protein_id="HDI6042636.1"
                     /translation="MNILQFNVRLAEGGAAGVALDLHQRALQQGLASHFVYGYGKGGK
                     ESVSHQNYPQVIKHTPRMTAMANIALFRLLNRDLFGNFNELYRTITRTPGPVVLHFHV
                     LHSYWLNLKSVVRFCEKVKNHKTDVTLVWTLHDHWSVTGRCAFTDGCEGWKTGCQKCP
                     TLNNYPPVKIDRAHQLVAGKRQLFREMLALGCQFISPSQHVADAFNSLYGPGRCRIIN
                     NGIDMATEAILADLPPVRETQDKPKIAVVAHDLRYDGKTNQQLVREMMALGDKIELHT
                     FGKFSPFTAGNVVNHGFETDKRKLMSALNQMDALVFSSRVDNYPLILCEALSIGVPVI
                     ATHSDAAREVLQKSGGKTVSEEEVLQLVQLSKPEIAQAIFGTTLAGFSQRSRAAYSGQ
                     QMLEEYVNFYQNL"
     gene            complement(7026..7514)
                     /gene="wcaB"
                     /locus_tag="PN977_002618"
     CDS             complement(7026..7514)
                     /gene="wcaB"
                     /locus_tag="PN977_002618"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaB"
                     /protein_id="HDI6042637.1"
                     /translation="MLEDLRANSWSLRPCCMVLAYRVAHFCSVWRKKNVLNNLWAAPL
                     LVLYRIITECFFGYEIQAAATIGRRFTIHHGYAVVINKNVVAGDDFTIRHGVTIGNRG
                     ADNMACPHIGNGVELGANVIILGDITLGNNVTVGAGSVVLDSVPDNALVVGEKARVKV
                     IK"
     gene            complement(7517..8356)
                     /gene="wcaA"
                     /locus_tag="PN977_002619"
     CDS             complement(7517..8356)
                     /gene="wcaA"
                     /locus_tag="PN977_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaA"
                     /protein_id="HDI6042638.1"
                     /translation="MKDNPLISIYMPTWNRQQLAIRAIKSVLRQDYNNWEMIIVDDCS
                     TSWEQLQQYVTALNDPRITYIHNDINSGACAVRNQAIMLAQGEYITGIDDDDEWTPNR
                     LSVFLAHKQQLVTHAFLYANDYVCQGEVYSQPASLPLYPKSPYSRRLFYKRNIIGNQV
                     FTWAWRFKECLFDTKLKAAQDYDIFLRMVVEYGEPWKVEEATQILHINHGEMQITSSP
                     KKFSGYFHFYRKHKDKFDRASKKYQLFTLYQIRNKRMTWRTLLTLLSVRNGKRLADGI
                     RGR"
     gene            complement(8448..10610)
                     /gene="wzc"
                     /locus_tag="PN977_002620"
     CDS             complement(8448..10610)
                     /gene="wzc"
                     /locus_tag="PN977_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase Wzc"
                     /protein_id="HDI6042639.1"
                     /translation="MTEKVKQHAAPVTGSDEIDIGRLVGTVIEARWWVIGITAVFALC
                     AVVYTFFATPIYSADALVQIEQNSGNSLVQDIGSALANKPPASDAEIQLIRSRLVLGK
                     TVDDLDLDIAVSKNTFPIFGAGWDRLMGRQNETVKVTTFNRPKEMADQVFTLNVLDDK
                     NYTLSSDGGFSARGQAGQMLKKEGVTLMVEAIHSSPGSEFTVTKYSTLGMINQLQNSL
                     TVTENGKDAGVLSLTYTGEDREQIRDILNSIARNYQEQNIERKSAEASKSLAFLAQQL
                     PEVRSRLDVAENKLNAFRQDKDSVDLPLEAKAVLDSMVNIDAQLNELTFKEAEISKLY
                     TKVHPAYRTLLEKRQALEDEKAKLNGRVTAMPKTQQEIVRLTRDVESGQQVYMQLLNK
                     EQELKITEASTVGDVRIVDPAITQPGVLKPKKGLIILGAIILGLMLSIVAVLLRSLFN
                     RGIESPQVLEEHGISVYASIPLSEWQKARDSVKTIKGIKRYKQSQLLAVGNPTDLAIE
                     AIRSLRTSLHFAMMQAQNNVLMMTGVSPSIGKTFVCANLAAVISQTNKRVLLIDCDMR
                     KGYTHELLGTNNVNGLSEILIGQGDITTAAKPTSIAKFDLIPRGQVPPNPSELLMSER
                     FAELVKWASKNYDLVLIDTPPILAVTDAAIVGRHVGTTLMVARYAVNTLKEVETSLSR
                     FEQNGIPVKGVILNSIFRRASAYQDYGYYEYEYKSDAK"
     gene            complement(10613..11056)
                     /gene="wzb"
                     /locus_tag="PN977_002621"
     CDS             complement(10613..11056)
                     /gene="wzb"
                     /locus_tag="PN977_002621"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase Wzb"
                     /protein_id="HDI6042640.1"
                     /translation="MFNNILVVCVGNICRSPTAERLLQRYHPELKVESAGLGALVGKG
                     ADPTAISVAAEHQLSLEGHCARQISRRLCRNYDLILTMEKRHIERLCEMAPEMRGKVM
                     LFGHWDNECEIPDPYRKSRETFAAVYTLLERSARQWAQALNAEQV"
     gene            complement(11062..12201)
                     /gene="wza"
                     /locus_tag="PN977_002622"
     CDS             complement(11062..12201)
                     /gene="wza"
                     /locus_tag="PN977_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein Wza"
                     /protein_id="HDI6042641.1"
                     /translation="MMKSKMKLMPLLVSVTLISGCTVLPGSNMSTMGKDVIKQQDADF
                     DLDKMVNVYPLTPRLIDQLRPRPNVARPNMTLESEIANYQYRVGPGDVLNVTVWDHPE
                     LTTPAGQYRSSSDTGNWVQPDGTMFYPYIGKVHVVGKTLAEIRSDITGRLATYIADPQ
                     VDVNIAAFRSQKAYISGQVNKSGQQAITNVPLTILDAINAAGGLTDTADWRNVVLTHN
                     GREERISLQALMQNGDLNQNRLLYPGDILYVPRNDDLKVFVMGEVKKQSTLKMDFSGM
                     TLTEALGNAEGIDMTTSNASGIFVIRPLKGEGGRNGKIANIYQLDMSDATSLVMATEF
                     RLQPYDVVYVTTAPVSRWNRLINQLLPTISGVRYMTDTASDIHNW"
     gene            12510..12659
                     /locus_tag="PN977_002623"
     CDS             12510..12659
                     /locus_tag="PN977_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6042642.1"
                     /translation="MVIEYWLSNNAGGSVTANGIAGKVTIRQLAIFKNYIKCHSIWFL
                     GVSLG"
     gene            12860..14443
                     /locus_tag="PN977_002624"
     CDS             12860..14443
                     /locus_tag="PN977_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HDI6042643.1"
                     /translation="MEWIADPSIWAGLITLIVIELVLGIDNLVFIAILAEKLPPKQRD
                     RARVTGLLLAMLMRLLLLASISWLVTLTQPLFSFRSFTFSARDLIMLFGGFFLLFKAT
                     MELNERLEGKDSNNPTQRKGAKFWGVVTQIVVLDAIFSLDSVITAVGMVDHLLVMMAA
                     VVIAISLMLMASKPLTQFVNSHPTIVILCLSFLLMIGFSLVAEGFGFVIPKGYLYAAI
                     GFSVMIEALNQLAIFNRRRFLSANQTLRQRTTEAVMRLLSGQKEDAELDAETASMLVD
                     HGNQQIFNPQERRMIERVLNLNQRTVSSIMTSRHDIEHIDLNAPEEEIRQLLERNQHT
                     RLVVTDGDDAEDLLGVVHVIDLLQQSLRGEPLNLRVLIRQPLVFPETLPLLPALEQFR
                     NARTHFAFVVDEFGSVEGIVTLSDVTETIAGNLPNEVEEIDARHDIQKNADGSWTANG
                     HMPLEDLVQYVPLPLDEKREYHTIAGLLMEYLQRIPKPGEEVQVGDYLLKTLQVESHR
                     VQKVQIIPLHKDGEMEYEV"
     gene            14781..15002
                     /locus_tag="PN977_002625"
     CDS             14781..15002
                     /locus_tag="PN977_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001014548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HDI6042644.1"
                     /translation="MNGTAFDLTYSEYNGAAARDADGIYRVMYIAKFEEAVYVLHCFQ
                     KKTQTTSQSDVDLAKRRYKELVQERKNEN"
     gene            14992..15282
                     /locus_tag="PN977_002626"
     CDS             14992..15282
                     /locus_tag="PN977_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HDI6042645.1"
                     /translation="MKIETFDSVWDAVSDTPEQAENMRIRAELVTIINNWIEQQGFSQ
                     AQAASALGVTQPRISELARGKIQIFSIDKLITMMAHAGLHIQRIEIQYPHAA"
     gene            complement(15334..17187)
                     /gene="asmA"
                     /locus_tag="PN977_002627"
     CDS             complement(15334..17187)
                     /gene="asmA"
                     /locus_tag="PN977_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane assembly protein AsmA"
                     /protein_id="HDI6042646.1"
                     /translation="MRRFLTTLMILLVVLVAGLSALVLLVNPNDFRDYMVKQVAARSG
                     YQLQLDGPLRWHVWPQLSILSGRMSLTAQGASQPLVRADNMRLDVALLPLLSHQLSVK
                     QVMLKGAVIQLTPQTEAVRSEDAPVAPRDNTLPDLSDDRGWSFDISSLKVADSVLVFQ
                     HEDDEQVTIRNIRLQMEQDPQHRGSFEFSGRVNRDQRDLTISLNGTVDASDYPHDLTA
                     AIEQINWQLQGADLPKQGIQGQGSFQAQWQESHKRLSFNQISLTANDSTLSGQAQVTL
                     TEKPEWQLRLQFPQLNLDNLIPLNETANGENGAAQQGQSQSTLPRPVISSRIDEPAYQ
                     GLQGFTADILLQASNVRWRGMNFTDVATQMTNKSGLLEITQLQGKLNGGQVSLPGTLD
                     ATSINPRINFQPRLENVEIGTILKAFNYPISLTGKMSLAGDFSGADIDADAFRHNWQG
                     QAHVEMTDTRMEGMNFQQMIQQAVERNGGDVKAAENFDNVTRLDRFTTDLTLKDGVVT
                     LNDMQGQSPVLALTGEGMLNLADQTCDTQFDIRVIGGWNGESKLIDFLKETPVPLRVY
                     GNWQQLNYSLQVDQLLRKHLQDEAKRRLNDWAERNKDSRNGKDVKKLLEKM"
     gene            complement(17209..17790)
                     /gene="dcd"
                     /locus_tag="PN977_002628"
     CDS             complement(17209..17790)
                     /gene="dcd"
                     /locus_tag="PN977_002628"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004942378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HDI6042647.1"
                     /translation="MRLCDRDIEAWLDEGRLSINPRPPVERINGATVDVRLGNKFRTF
                     RGHTAAFIDLSGPKDEVSAALDRVMSDEIVLDESEAFYLHPGELALAVTLESVTLPAD
                     LVGWLDGRSSLARLGLMVHVTAHRIDPGWSGCIVLEFYNSGKLPLALRPGMLIGALSF
                     EPLSGPAARPYNRREDAKYRNQQGAVASRIDKD"
     gene            complement(17882..18523)
                     /gene="udk"
                     /locus_tag="PN977_002629"
     CDS             complement(17882..18523)
                     /gene="udk"
                     /locus_tag="PN977_002629"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HDI6042648.1"
                     /translation="MTDQSHQCVIIGIAGASASGKSLIASTLYRELREQVGDEHIGVI
                     PEDCYYKDQSHLSMEERVKTNYDHPSAMDHSLLLEHLQALKRGSAIDLPVYSYVEHTR
                     MKETVTVEPKKVIILEGILLLTDARLRDELNFSIFVDTPLDICLMRRIKRDVNERGRS
                     MDSVMAQYQKTVRPMFLQFIEPSKQYADIIVPRGGKNRIAIDILKAKISQFFE"
     gene            18841..22158
                     /locus_tag="PN977_002630"
     CDS             18841..22158
                     /locus_tag="PN977_002630"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HDI6042649.1"
                     /translation="MSKQSQHVLIALPHPLLHLVSLGLVSFIFTLFSLELSQFGTQLA
                     PLWFPTSIMMVAFYRHAGRMWPGIALSCSLGNIAASILLFSTSSLNMTWTTINIVEAV
                     VGAVLLRKLLPWYNPLQNLADWLRLALGSAIVPPLLGGVLVILLTPGDDPLRAFLIWV
                     LSESIGALALVPLGLLFKPHYLLRHRNPRLLFESLLTLAITLTLSWLSMLYLPWPFTF
                     IIVLLMWSAVRLPRMEAFLIFLTTVMMVSLMMAADPSLLATPRTYLMSHMPWLPFLLI
                     LLPANIMTMVMYAFRAERKHISESETRFRNAMEYSAIGMALVGTEGQWLQSNKALCQF
                     LGYSQEELRGLTFQQLTWPEDLNKDLQQVEKLISGEINTYSMEKRYYNRNGDVVWALL
                     AVSLVRHTDGTPLYFIAQIEDINELKRTEQVNQQLMERITLANEAGGIGIWEWELKPN
                     IFSWDKRMFELYEIPPHIKPNWQVWYECVLPEDRQHAEKVIRDSLQSRSPFKLEFRIT
                     VKDGIRHIRALANRVLNKEGEVERLLGINMDMTEVKQLNEALFQEKERLHITLDSIGE
                     AVVCIDMAMKITFMNPVAEKMSGWTQEEALGVPLLTVLHITFGDNGPLMENIYSADTS
                     RSAIEQDVVLHCRSGGSYDVHYSITPLSTLDGSNIGSVLVIQDVTESRKMLRQLSYSA
                     SHDALTHLANRASFEKQLRILLQTVNSTHQRHALVFIDLDRFKAVNDSAGHAAGDALL
                     RELASLMLSMLRSSDVLARLGGDEFGLLLPDCNVESARFIATRIISAVNDYHFIWEGR
                     VHRVGASAGITLIDDNNHQAAEVMSQADIACYASKNGGRGRVTVYEPQQAATNSERAV
                     MSLDEQWRMIKENQLMMIAHGVASPRIPQARNLWLISLKLWSCEGEIIDEQTFRRSFS
                     DPALSHALDRRVFHDFFQQTAKAIASKGLSIALPLSVAGLSSATLVNELIEQLENSPL
                     PPRLLHLIIPADAILDHAESVQKLRLAGCRIVFSQVGRDLQIFNSLKANMADYLLLDG
                     ELCANVQGNLMDEMLITIIQGHAQRLGMKTIAGPVVLPLVMDTLSGIGVDLIYGDVIA
                     DAQPLDLLVNSSYFAIN"
     gene            complement(22268..23116)
                     /gene="alkA"
                     /locus_tag="PN977_002631"
     CDS             complement(22268..23116)
                     /gene="alkA"
                     /locus_tag="PN977_002631"
                     /EC_number="3.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase 2"
                     /protein_id="HDI6042650.1"
                     /translation="MYTLNWQPPYDWSWMLGFLAARAVNGVETVADSYYARSLAVGEY
                     RGVVTAIPDIASHTLHINLSAGLEPVAAECLAKMSRLFDLQCNPQIVNGALGKLGAAR
                     PGLRLPGCVDAFEQGVRAILGQLVSVAMAAKLTARVAQLYGERLDDFPDYVCFPTPQR
                     LAAADPQALKALGMPLKRAEALIHLANATLEGTLPMTIPSDVEQAMKTLQTFPGIGRW
                     TANYFALRGWQAKDVFLPDDYLIKQRFPGMTPAQIRRYAERWKPWRSYALLHIWYTEG
                     WQPDGA"
     gene            23250..24602
                     /gene="yegD"
                     /locus_tag="PN977_002632"
     CDS             23250..24602
                     /gene="yegD"
                     /locus_tag="PN977_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HDI6042651.1"
                     /translation="MFIGFDYGTANCSVAVMRDGKPHLLKMENDSTLLPSMLCAPTRE
                     AVSEWLYRHHDVPADDDETQALLRRAIRYNREEDIDVTAKSVQFGLSSLAQYIDDPEE
                     VWFVKSPKSFLGASGLKPQQVALFEDLVCAMMLHIRQQAQAQLPEAITQAVIGRPINF
                     QGLGGDEANAQAQGILERAAKRAGFKDVVFQYEPVAAGLDYEATLQEEKRVLVVDIGG
                     GTTDCSLLLMGPQWRSRLDREASLLGHSGCRIGGNDLDIALAFKNLMPLLGMGGETEK
                     GIALPILPWWNAVAINDVPAQSDFYSSANGRLLNDLVRDAREPEKVALLQKVWRQRLS
                     YRLVRSAEESKIALSSVAETRASLPFISDELATLISQQGLENALSQPLARILEQVQLA
                     LDNAQEKPDVIYLTGGSARSPLIKKALAEQLPGIPIAGGDDFGSVTAGLARWAEVVFR
                     "
     gene            complement(24615..26561)
                     /gene="yegI"
                     /locus_tag="PN977_002633"
     CDS             complement(24615..26561)
                     /gene="yegI"
                     /locus_tag="PN977_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YegI"
                     /protein_id="HDI6042652.1"
                     /translation="MKTNIKVFTSTDELTTLGRELGKGGEGAVYDIEEFVDSVAKIYH
                     TPPPALKQDKLAFMAATADAQLLNYVAWPQATLHGGRGGKVIGFMMPKVSGKEPIHMI
                     YSPAHRRQSYPHCAWDFLLYVARNIASSFATVHEHGHVVGDVNQNSFMVGRDSKVVLI
                     DSDSFQINANGTLHLCEVGVSHFTSPELQTLSSFVGFERTANHDNFGLALLIFHVLFG
                     GRHPYSGVPLISDAGNALETDIAHFRYAYASDNQRRGLKPPPRSIPLSMLPGDVEAMF
                     QQAFTESGVATGRPTAKAWVAALDSLRQQLKKCTVSAMHVYPGHLADCPWCALDNQGV
                     IYFIDLGEEVITTSGDFVLAKVWAMVMASVAPPALQLPLPDHFQPTGRPLPLGLLRRE
                     YIILIEIALSALSLLLCGLQTEPRYIILVPVLSAIWIIGSLTSKAYKAEIQQRREAFN
                     RAKMDYDHLVSQIQQLGGLEGFIAKRTMLEKMKDEILGLPEEEKRDLAALQDTARERQ
                     KQKFLEGFFIDVASIPGVGPARKAALRSFGIETAADVTRRSVKQVKGFGDHLTQAVID
                     WKASCERRFVFRPNEAVTPADRQAVLTKMAAKRHRLESALTVGATELQRFRLHAPART
                     MPLMEPLRQAAEKLAQAQADLSRC"
     gene            26763..27224
                     /locus_tag="PN977_002634"
     CDS             26763..27224
                     /locus_tag="PN977_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegJ family protein"
                     /protein_id="HDI6042653.1"
                     /translation="MKKILLMLSLLFFTTASYCEISDTLVTGGYDKKAMSDAIKHARK
                     ETDQFIEVMNKKDADTFAVKAPITDRGETEHFWLTDVTYSNGMFIGIISNDPGIVTNV
                     EYGQEWKIKKEDISDWMYTRGDKIYGGYTIDPLLVTYPKEEADELRAKLVR"
     gene            complement(27289..>27763)
                     /locus_tag="PN977_002635"
     CDS             complement(27289..>27763)
                     /locus_tag="PN977_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HDI6042654.1"
                     /translation="FAEAEAKELAVRDFACTFLGLISSANGTLIMQIGDGGVVVDLGH
                     GLQLPLTPMVGEYANMTHFITDDDAVSRLEIYTSTERAHKVAAFTDGIQRLALNMLDN
                     SPHVPFFTPFFNGLASATQEQLDLLPELLKQFLSSPAVNERTDDDKTLALAMWTE"
BASE COUNT         6653 a         7266 c         6990 g         6854 t
ORIGIN      
        1 agaatgattt ttttgttatc cggcaggcca agctggttac gaagggcgtc aacatcggcg
       61 tctgcaacat gttgaaaacg ggcgatttcc gaccagttgg ggaagaagat gacgttttcc
      121 gccgccacgc ctttttcgat agctttattc atcatcgagc gcgaaatcgt ggagacgtta
      181 tcgacgttat gcagtccgct acgttcgaac gccgtcgcca gctgtgccac tttgccgcct
      241 ttactttttc cggcaaggcc cagccccagc atggcgtcca cttcgtaatc ctgaatatgt
      301 agcaccgtgc gcgcaccaga cagtttcacc agcaggcgca ttcccggcgt gcaaaacagc
      361 gttggtacca cgccgataat gcgatccggc ttccagcgac gttgcgccat cagcggaaag
      421 aaactgctgg cggcaaaact gccgagatgc aacaggcgtt tcagggtgct cggctgtttt
      481 ggcacgtaca gcgggcagcg ccacaccgtg gcggccccct cttcccgttt gtaccgccag
      541 gcggaatagt tctcgcccac ctgccattgc gggtagtaag gcggtgcggt aataacccgc
      601 acctcatgac cttctgccgc cagccattcc accatctcgc cggtgtattt gccgatgccg
      661 gttaactccg gcgagtagtt aatgccgtag accagtattt tcataatccg ggtactccgg
      721 cacgcttctc agcgagaaaa taggcgcggc tgttagcgtg aacattgtca ctagcgagca
      781 gtgcgtccgg cgtcagccag cggtaatcgt catgctgctc atccggcagt aacagctctt
      841 cttccgcgac tctgaagcga aaaccgagca ccacatagtg agtggtaaaa tccgtgccgg
      901 agaagttatc gtcataaaaa tgctgccaga caccgtaaaa ctggcctgct gttatcggca
      961 gacgcagccc cagttccgcc atcgtcagcc gctcaaatgc ggcttccagc gtttcgtctt
     1021 tctgcacgcg ccctcccggc acaaaccagt aaccctgcgc cgggcggttg gttcttttgc
     1081 caagcagaaa ctcgccgcga ctgttctcga caataaagtc gagagagaca agcggtgtgg
     1141 agcgcactac cgtggcaaag tcttcctgac gtaaaaacat cattaccccc gaaagcggtc
     1201 ttgattctca aggaaccact ggtaagtgct ggcgagcccc gcttccagtg agatttcgtg
     1261 ataccagcca agctgatgca ggcgtgtcac atccagcagt ttgcgcggtg taccatccgg
     1321 tttactggca tcaaaaacca cccgaccttt gtaacccacc actttggcga tggtttgcgc
     1381 cagctcgcgg atagtgcagt caacgcccgt gccgacgtta atgtgcgaca gcatcggttg
     1441 ggtgttctcc agccagactt cgtgcgccag ctccatcaca tgaatgctcg ccgccgccat
     1501 atcatcgacg tgcagaaatt cgcgcatcgg tgtaccgctg ccccacacca ccacgtccgg
     1561 tgcattctgt gccgtcgcct cgtggaagcg acgcagcaat gcagggatca catgcgaatt
     1621 actcgggtgg aagttgtcat gcgggccata caggttggtc ggcatgaccg agcggtaatc
     1681 gcgtccatac tggcggttgt aagattcgca cagtttgatc ccggcgattt tggcaatagc
     1741 ataaggctcg ttagtcggct ccagcgtacc ctgcaataac tcgctttctg ccatcggctg
     1801 ttttgccagt ttcgggtaga tacaggacga tccgagaaac agcagtttgt tcacgtcgtt
     1861 ctgatgcgcg gcgtgaatga tgttgctctc aatcatcatg ttctgataga tgaaatccgc
     1921 cggatacgtg ttgttggcaa caatgccgcc cactttcgcc gccgccagat agacctggtc
     1981 aatacgttcg ctggcaaaga aatcatgcac cgcgcggctg tccagcaggt tcagttcatc
     2041 gcgggtgcgt aataccagct ctacatcacc gcgctgttcg agctgccgcc tgatggcgga
     2101 accgaccatc ccgcgatggc cagcaataaa aatgcgttgt ttactcatgc ttatgactcc
     2161 agcgcgatcg ccacgtcgta gccgtgagat ttcagcagag agtgtttttt cgccgcttcg
     2221 aggtcattag ccaccatttc agacaccatc tctctgaggg tgatttccgg tttccagccc
     2281 agtttttcgt gcgctttggt cgggtcgccg agcagcgttt caacttcagc cggacggaag
     2341 taacgcgggt caacggcgat aatcacatca cccggtttaa cgcccggcgc gtcatgcccg
     2401 gtgacggaaa ccacaatgcc cttctcttca acgcccgtgc cttcaaagcg cagtttgatg
     2461 cccagctgtg ctgccgccat ttccacgaac tgacgtacgg agtactgaac gccggtagca
     2521 ataacgaaat cttccggttg ttcctgttgc agcatcatcc actgcatttt tacgtagtct
     2581 ttggcatggc cccagtcacg cagggaatcc atgttgccga ggtacaggca cgactccagc
     2641 ccctgggcga tgttggcgat tgcgcgggtg attttgcggg taacgaaggt ttcgccacgg
     2701 cgcggggatt catggttgaa gagaataccg ttacaggcgt acatgccgta ggattcgcgg
     2761 tagttaacgg tgatccagta ggcgtacagt ttggcgaccg catacggaga gcgtgggtag
     2821 aacggcgtgg tctctttctg cggaatttcc tgcaccagac cgtacagttc agaagtagaa
     2881 gcctgataga aacgggtttt cttttccaga ccgaggaagc ggatcgcctc gagcaggcgc
     2941 agcgtaccca tcgcatcgac gtctgcggta tattccggtg actcaaaaga aaccgcaacg
     3001 tggctcattg cgcccaggtt gtacacttca tccggctgca cttcacgcaa aatgcgcgtc
     3061 aggttggagg tatcactcag gtcgccataa tgcagatgga atttcggatt gcaggtgtgc
     3121 ggatcctgat aaatgtgatc cacgcgctcg gtgttgaatg acgatgcgcg acgcttaata
     3181 ccatgcacct cgtaaccttt ttccagcaga aactctgcca ggtaagaacc gtcttgtccg
     3241 gttacgccgg tgatgagagc gacttttgac atgtcttatt cctctgtatt ttttgaattt
     3301 attcagtttc aacgcgtttg cgtatcacca ctgcggggtt cccccggcaa accacatttg
     3361 ccggaagcga tttaaaaaca ctgcttcgcg cacccacgac ggtgccgttg ccgatcgtga
     3421 cgccaggggc aacaaaaaca tctgtcgcca gccagcattt ctcgccaatc acaataggcg
     3481 tggcgttaat ggtgaaatgt tgacttgcat ggtcgtggct cccggtgcat aaataacttt
     3541 tttgcgatat caccgaatgt gcgccaatga ttatttcacc gagggtatat aaattgacgt
     3601 catcgccgac ccacgcgtaa tcacctaagg ttaatttcca cggataggta atttttactg
     3661 acggacgaat cactacgttt tttcctattt ttgcgccgaa taaacgtaat aaaaatgccc
     3721 gccagcgata caatacttgt ggcgaccagg caaataatgt tgcctgtact gcccaccaca
     3781 attgcacttt aatagcgttg ccgccccgga accctttcgg caccgagaac ccacttaaat
     3841 cctgcatcgt tttcccttat attcagcttt tgttatataa ggctttcgtc tttgaggtag
     3901 tacgctggcg taaatgccag gataattctg cccagaagcc aggcacatgt aatatctgcc
     3961 gttggacttt tttggcgtca gcacataatt ccatattatt ggtggtagat actcccccca
     4021 tagaaaattc agacaccagg ccattgagtt ttctaaatgc ataaccagct ttatacattt
     4081 tggctgccag cgcgtaatcg gaagaaactt tatattccag gtcataacgc cattttttca
     4141 agccggatac cggaaaaaat atcgcctgat gactggcggg caggctgtga taaatatacc
     4201 agcccggttt ggcgctacgt ttaattttat gcccgtcgcc aaaatccagc agcgcatcgc
     4261 cggtgatcat cacgttatct ttttgtactt ttaacttacg gacaaaattt gcggcatcct
     4321 gatgaaaaat atcacctgag ttgagaaaca acgcgaactt gccttgtgcc atcgcaatgc
     4381 ccttgttcat ggcgtcgtag ataccgttat ctggttcgct gacaaagcgt aggttataga
     4441 tacccttgag attttccagg tactcacggg tgccgtcatt ggaaccgcca tcgacaacaa
     4501 tccattcgaa gctgatatcg tccgcctgcg ccagatgcgc cagcgaggca tgtgttttga
     4561 ctatccctgc gaggttacga aacgcgacag tgattatgct aagcaacatg ttcttattct
     4621 tacctcgtaa tatttaacgc ttttcgcaaa ataaacggac agacgattaa aaatgcatat
     4681 tccgggctaa atatcgaacc ggtaaaaaac agcgacaccg gagtaaaaag atataattgc
     4741 acgcgaaaat tgcggttatc accaaaagcg ttgatcatca tttttatcac tttccccatg
     4801 taccacagtg ataaaaacac cgcgaaccag gaaaaataaa taatcagcag atacaaacca
     4861 ttgtctatgg tttttccgac atccacaccg ttaaatattc cgaatgatgc gacatattca
     4921 taaagtgagc caaatctgac tacaccgtca atatgggtca aagaataacc gaccatcacc
     4981 agcggaccga caatacgata atacgatgac gacccttccg tacctaaatc gcccagacgg
     5041 gtggaaatat aaggaaacgc gattaccaca ccaaccagga atacagccag agaaaccaat
     5101 gctaacggta actttttctt aatcgcctct ttattcagat attgaaatgc ccactccagc
     5161 aaataaaaaa ggataaaggt cataacccct gaaaacgatc cggataatat tatccctgcg
     5221 agaatcatag catcggtttt aggcgttttg ataccaaact gtttgatgct gagccaaatt
     5281 gagattaatg ccagagcgaa aaatgccggt tcgaaataaa gtgctgtcgt gcgcttgccg
     5341 ccgaatttaa tgaaattcag cacatagctg ttgctgtaaa tcagatattt cgaaatcgac
     5401 tccataatat tgctaccgcc ggtgaggata atttgcgcca tctccaccgc cgccagcgcc
     5461 accaccagcc cgaccaccag ataaaagaaa cgtaatatct tgcgatggtt gtgtggcgaa
     5521 attgttttaa aacgaatact ccacaccatg ccaataatga tcacaatata gacaaacagc
     5581 atggttgaag tgacgtattt actggcatcc aacgactgac caaacaggta gttaaacgcc
     5641 gtgagtcccg caccaacccc taaggcaatc atcaattttt taacgttgat gcgctctaaa
     5701 aacagcagta acaagacggg taaaaaagtg acgatggtaa tggggaaact ttcgccaagc
     5761 tgggcgattt tgacgttaac cagcaggtaa atcagcggca gcagtaggta gctacagatt
     5821 ctgatagaag ttgacatact cctccagcat ctgttgtcca ctgtaggcgg cgcggctgcg
     5881 ttggctaaac ccagccagcg tggtaccaaa tatcgcctgc gcaatttccg gtttgcttaa
     5941 ctgcaccagt tgcagcacct cttcttcgct gacggtttta ccgccggatt tttgcagcac
     6001 ttcccgcgcc gcatcgctat gggtggcaat caccggcacg ccaatcgata gcgcctcaca
     6061 caaaatcagc gggtagttat cgacgcgaga actgaatacc aacgcatcca tctgattgag
     6121 cgcgctcatc aacttgcgct tgtcagtttc aaagccgtga ttaaccacgt tgccagcggt
     6181 gaacggcgag aacttaccaa aggtatgcag ttcaattttg tcgcccagcg ccatcatctc
     6241 acgtaccagt tgctggttag ttttgccgtc gtaacgcaga tcatgcgcca ccaccgcgat
     6301 tttcggcttg tcctgggttt cgcgtaccgg aggcaagtcc gccagaatcg cttcggttgc
     6361 catatcaatg ccattattga taatccggca acgccctgga ccgtacagac tattgaaagc
     6421 atcagccaca tgctggctgg gggaaataaa ctgacagccc agcgccagca tctcacggaa
     6481 taactggcgt ttgcccgcca ccagttggtg tgcgcgatca atcttcaccg gcggataatt
     6541 atttaaggtc gggcatttct ggcagcccgt tttccagcct tcgcaaccgt cggtaaaggc
     6601 gcagcgtccg gtaacgctcc agtggtcgtg tagcgtccag accagagtga catccgtttt
     6661 atggtttttc actttttcgc aaaagcgcac cacgctctta agattcaacc agtagctgtg
     6721 cagcacatga aaatgcagga ccaccggacc cggtgtgcga gtaatggtgc gatataactc
     6781 attgaaattg ccgaacagat cgcgattaag cagacgaaac aacgcaatgt tcgccatcgc
     6841 ggtcatccgc ggcgtatgtt tgatgacctg cggatagttc tgatggctga cgctctcttt
     6901 gccacctttg ccgtagccgt agacaaaatg tgacgccagc ccctgttgca gcgcgcgctg
     6961 gtggagatct aacgccacac ctgctgcccc gccttccgcc agtcgcacat tgaattgcag
     7021 aatattcatt taattacctt cactcgcgct ttttctccca ccaccagcgc gttgtccggg
     7081 acagagtcga gcaccacgct gcccgcgccc acggtgacgt tgttaccaag cgtgatatca
     7141 ccaagaataa tgacgttggc accgagttcg acgccgttgc caatgtgtgg acatgccatg
     7201 ttatcggcac cacgattgcc gatagtgacg ccgtgacgaa tggtgaaatc atcccccgcg
     7261 accacgtttt tattgatcac cacggcgtaa ccgtgatgga tagtgaaacg gcggccaatg
     7321 gtcgcggcgg cctggatttc ataaccgaaa aagcattcgg tgataatgcg atacagcacc
     7381 agcagcgggg ccgcccacag attgttgagg acgttctttt tgcgccacac cgaacaaaaa
     7441 tgagcgacgc gataggcaag aaccatgcag cacggacgta aactccagct gttggcgcgc
     7501 agatcttcca gcatctttag cgcccccgaa taccatcagc cagacgtttg ccgttacgca
     7561 ccgacagcag cgtcagcaaa gtgcgccagg tcatgcgttt attgcggatc tggtagaggg
     7621 taaacagctg atattttttg ctggcgcggt cgaatttgtc tttgtgcttg cggtaaaagt
     7681 ggaagtatcc ggagaatttt ttcggtgacg aggtgatctg catttcacca tgattgatat
     7741 gcaggatctg cgtcgcctct tccactttcc acggttcgcc gtactccact accatccgca
     7801 ggaagatgtc gtaatcctgt gccgctttca gtttggtatc gaacaagcac tctttgaaac
     7861 gccacgccca ggtaaagacc tgattgccaa taatattgcg tttgtagaac aggcggcgtg
     7921 aatacggcga tttgggatac agcggcaagc ttgccggttg ggaatagact tcgccctggc
     7981 aaacgtagtc gttagcgtac aaaaaggcgt gagtgaccag ttgctgttta tgggcgagga
     8041 agacgctcag gcggttgggt gtccattcat catcatcatc gatcccggtg atgtattccc
     8101 cttgcgccag cataatcgcc tggttacgta ccgcgcacgc cccgctgtta atgtcgttgt
     8161 gaatgtaagt gatgcgcgga tcgttgaggg cggtgacgta ctgttgtagt tgttcccagg
     8221 aggtggagca atcatccacg ataatcatct cccagttgtt gtagtcctgg cgcaggaccg
     8281 attttatcgc ccgaatagcc agttgttggc ggttccaggt cggcatatag attgaaatca
     8341 gcggattgtc tttcataggt tgttctcacg gcttatgagc tggtttgctt gccggatgcg
     8401 gcgtgaacgc cttatccggc ctacggggcg gtgcgaatgc agcgtcgtta tttcgcatcc
     8461 gacttatatt cgtattcgta atagccataa tcctgatacg cgctggcgcg gcggaagatg
     8521 gagttcagaa tcaccccttt caccggaata ccattttgct caaagcggct cagactggtt
     8581 tccacttctt tcaatgtgtt gaccgcataa cgcgccacca ttaacgtggt tccgacatga
     8641 cgaccaacaa ttgccgcatc ggtcactgcc agaatcggcg gcgtatcaat caacaccagg
     8701 tcgtagtttt tactcgccca cttcaccagt tcggcaaagc gttcgctcat taacagttca
     8761 gaaggatttg gcggcacctg accgcgcggg atcaggtcaa atttggcaat agaggtcggt
     8821 ttagcagcag tagtaatatc gccctgtcca atcagaattt ccgacaggcc attaacgtta
     8881 ttagtgccca acagctcgtg ggtgtagcct ttgcgcatat cgcagtcgat caacaacacc
     8941 cgtttattgg tctggctaat caccgccgcc aggttggcgc agacaaaggt tttaccgatt
     9001 gacgggctaa ccccggtcat catcaacaca ttgttctgcg cctgcatcat cgcgaagtgc
     9061 agactggtac gcagactacg gatggcttca atcgccagat cggttggatt ccccaccgcc
     9121 agtagctggc tctgtttata gcgtttaatc cctttgatgg ttttgacgct atcgcgcgct
     9181 ttctgccact ccgacagcgg gatgctggca tagacgctaa tgccatgttc ttccagcact
     9241 tgcgggcttt caatgccgcg attaaacaac gagcgcagca gcacagccac gatagagagc
     9301 atcaggccaa ggataatcgc cccaaggata atcagccctt tcttcggttt tagcacacct
     9361 ggctgggtga ttgccgggtc aacaatgcgc acatcgccga cggtgctggc ttcggtgatt
     9421 ttcagctcct gctctttatt cagcagctgc atatagacct gctgcccaga ctcgacatcg
     9481 cgggtcagac ggacaatctc ctgctgggtt ttcggcatcg ccgttacgcg accgttcagt
     9541 ttggcttttt cgtcttccag cgcctgccgt ttctccagca gcgtgcgata cgccgggtga
     9601 actttggtgt acagcttgga gatttccgct tctttaaagg tcagttcgtt caattgtgcg
     9661 tcgatgttca ccatcgaatc gagcaccgct tttgcttcca gcggcagatc aacagaatct
     9721 ttatcctgac ggaaggcatt caatttattt tcggcaacat caaggcggct acgtacttcc
     9781 ggtaactgtt gcgcgaggaa ggcgaggctt ttcgacgctt ccgcggattt gcgctcaata
     9841 ttttgttcct gatagttacg ggcgatgctg ttaagaatgt cgcggatctg ttcgcgatct
     9901 tcaccggtat aagtcaggct cagtacgcct gcgtctttgc cgttctccgt taccgtcagg
     9961 ctgttttgca gttggttgat catccccagc gtggagtatt tggtgacggt aaactcactg
    10021 cccgggctgg agtgaatggc ttcaaccatc agcgtgacgc cttctttttt cagcatctgg
    10081 cccgcttgcc cacgggcgct aaagccgcca tcgctgctca gagtgtagtt tttatcgtcc
    10141 agcacattaa gcgtaaacac ctgatccgcc atctctttcg ggcggttaaa ggtggtcact
    10201 tttaccgtct cgttctggcg tcccatcagg cgatcccagc ccgcaccgaa aatagggaac
    10261 gtgtttttgc tcactgcaat atcgaggtcg agatcgtcca ccgttttacc aagcaccaga
    10321 cgcgaacgaa tcaactggat ctcggcgtcc gatgctggcg gtttgttggc taatgccgaa
    10381 ccaatgtcct gcactaacga attgccgctg ttttgctcga tttgtaccag tgcgtcggca
    10441 ctataaatcg gcgtggcgaa gaaggtgtaa accacggcac agagggcaaa tacggcggtg
    10501 atgccaatta cccaccagcg cgcttcaatg acggtgccga ccaggcgacc aatatcgatt
    10561 tcatcactgc ccgttaccgg agcggcatgt tgttttactt tttctgtcat tcttatacct
    10621 gctctgcgtt caatgcctgc gcccactggc gggcagatcg ttcaagtaat gtgtacaccg
    10681 ctgcaaacgt ttcccggctt ttgcgatacg gatcggggat ttcacattcg ttatcccagt
    10741 gaccaaacag catcactttg ccgcgcatct caggtgccat ctcgcataag cgttcgatat
    10801 ggcgcttttc catggtcaaa atcaggtcgt agttgcgaca caggcgacgg ctgatttgcc
    10861 gtgcacagtg accttccaga gacagttgat gttctgcggc gacgctgata gcagtagggt
    10921 cagcgccctt accgaccagc gcgccgagtc cagcggactc cactttcagc tccgggtgat
    10981 aacgttgcag taagcgttcc gccgtcgggg aacggcaaat attgccgaca cagacaacta
    11041 agatgttgtt aaacatgacg attaccagtt atgaatgtcg ctggctgtat ccgtcatgta
    11101 acggacaccg ctaatagttg gcagcaactg attgatcaga cggttccagc gggataccgg
    11161 ggcggtggtg acatacacca cgtcataagg ttgcaggcgg aattctgtcg ccatcaccag
    11221 cgacgtggca tcggacatat ccagctggta gatattggca atcttgccgt tacgcccgcc
    11281 ctcgcctttc aacggacgaa tgacaaagat gccgctggcg ttggaggtgg tcatgtcgat
    11341 accttcagca ttgcccaggg cttcagtcag ggtcatgccg ctaaagtcca ttttcagggt
    11401 gctctgtttc ttcacttcac ccatcacaaa cactttcaga tcatcattac gcggcacgta
    11461 gagaatatcg ccggggtaaa gcagtcggtt ctgattgagg tcgccgtttt gcatcagcgc
    11521 ctgcaaagaa atgcgctctt cacgaccatt gtgtgttagc accacgttgc gccagtcagc
    11581 ggtgtcggtc aggccacctg cggcgttgat ggcgtcgaga atggtcagtg gcacgttggt
    11641 gatcgcctgt tgaccggatt tattcacctg accggagata taggcttttt gcgagcggaa
    11701 ggcggcgata ttaacgtcca cctgcgggtc agcgatgtac gtcgctaagc gcccggtaat
    11761 atcactgcgg atttcagcga gcgttttccc caccacgtga actttgccga tatacgggta
    11821 gaacatggtg ccgtcaggct gtacccagtt gccggtgtcg ctggagctgc ggtactgacc
    11881 ggctggcgtg gtgagttccg ggtgatccca gacggtgaca ttaagaacgt ctcccggccc
    11941 gacgcgatac tgataattcg cgatctcact ttccagcgtc atattggggc gcgctacatt
    12001 cgggcgtggg cgtaattgat caatcaggcg cggggtcaac ggataaacat tcaccatttt
    12061 gtcgagatcg aaatcagcgt cctgctgttt gatgacgtct ttccccatcg tcgacatatt
    12121 gctgcccgga agtactgtgc aaccgcttat caaggttact gacaccaata atggcatcaa
    12181 tttcattttg gatttcatca ttgtttattt atcactttgg cagagtaatt atcctgtgca
    12241 ctattaatag caatgtcgcc atgcacattt accttgcagt taattgaata aaaatttaac
    12301 tggcatcagt cctaaaaaaa ttgatttcat ccgcaggcta ttgacagaat aattcagact
    12361 ggtctttcag gcatccagac acgctaccgc ccgtggcttt ttagctacca atacactgat
    12421 ttagcttaat ttttaaaacc ctctcaacat acagtcgttg atgagaaagg gttattgcgg
    12481 aaattaactt ccgaatataa ggtgacatta tggtaattga atattggctc tccaataatg
    12541 caggaggaag tgttacagct aacggaatag caggcaaggt aacaattcgg caattggcta
    12601 tttttaagaa ttatattaag tgtcattcaa tatggttttt aggagtttct ttaggttgac
    12661 aatatttaat atagcgtctc cacatgcgat atttcttaaa taacgtttta ttattaccac
    12721 ttataattcg gggataatgt gaggttataa ccctcaaata atatgagata aatagtgctg
    12781 taacattgca tttttgcccc gatttatcca tgatcgaatt gtgacatttg tcatacaacg
    12841 aataggtttt gtacttacta tggaatggat tgccgatccg tctatctggg ccgggttaat
    12901 cacgctgatt gtgatcgaac tggtcctcgg cattgataac ctggtcttta ttgccatcct
    12961 cgccgaaaaa ctaccgccga agcagcgcga ccgcgcacgg gttaccgggc tgctgctggc
    13021 gatgttaatg cgcctgttac tgctggcgtc aatctcctgg ctggtcaccc tgactcaacc
    13081 gctgttcagc ttccgctcgt ttacctttag cgcccgcgat ttaatcatgc tgtttggtgg
    13141 tttcttcctg ctgttcaaag ccacgatgga gctgaacgaa cggctggagg ggaaagacag
    13201 caataacccc acacaacgca aaggtgcgaa gttctggggg gtggtgacac aaattgtggt
    13261 actggacgcc atcttctcac tcgactcggt gattaccgcc gtcgggatgg tcgaccattt
    13321 actggtcatg atggccgccg tcgttatcgc tattagcctg atgttgatgg ccagcaagcc
    13381 cttaacgcaa tttgttaaca gtcacccgac gatcgttatt ctctgcttaa gcttcctgtt
    13441 gatgattggc tttagcctgg tggcagaagg tttcggcttc gtcataccga aaggctacct
    13501 gtacgctgct attggtttct cggtgatgat cgaggcgctc aatcagttgg ctatctttaa
    13561 ccggcgacgt tttctttccg ctaaccagac gctgcgccag cggactaccg aagcggtaat
    13621 gcgcctgctt agcgggcaaa aagaagatgc ggaactggac gccgaaaccg cgtccatgct
    13681 ggtggatcat ggtaaccagc agatctttaa tccgcaggaa cggcggatga ttgagcgggt
    13741 acttaatctt aaccagcgta ctgtcagcag cattatgacg tcgcgccacg atattgagca
    13801 tatcgatctc aacgcgccgg aagaagaaat ccgccagttg ctggagcgaa atcagcacac
    13861 gcgactggtg gttactgatg gcgatgacgc ggaagatttg ctcggtgttg ttcacgttat
    13921 cgacctgcta caacagtctc tgcgcggcga accgctcaac ctgcgggtgt tgattcgcca
    13981 gccgctggtg ttcccggaaa ccttgccgtt gttaccagcc ctggagcagt tccgtaatgc
    14041 ccgcacacac tttgcttttg tagtggatga gtttggctcg gtggaaggga ttgtgacatt
    14101 aagtgacgtc acagaaacca ttgccggtaa cttaccgaat gaagtggaag agatcgacgc
    14161 ccgccatgat attcagaaaa atgcggacgg ttcctggacg gcgaatggtc atatgccgct
    14221 ggaagatctg gtgcaatatg tgccgctgcc gctggatgaa aaacgtgaat atcacaccat
    14281 tgccgggctg ttgatggagt atttgcagcg tattccaaaa cctggcgaag aagttcaggt
    14341 gggggattat ttgcttaaaa cgttgcaggt ggaaagccat cgcgtgcaga aggtgcaaat
    14401 tattccgctg cataaggatg gcgagatgga gtacgaggtg tgatggtatc ggccatttta
    14461 cccaatgctg cgtaacgcct tataacttca gcaatgaagc gttgtctgtg gtacatggac
    14521 atcacagcgg gggtgtgggt ccggctgtaa atcgccgaat cgtttcctgt aggccggggc
    14581 agcccacatg gatgtgggct gagggcgcgt tttacaggga tgttacctcg cgcccgtacc
    14641 cgatagccga aaggaataag atgagggaac cgcgccagcg gcgatttacc gccgggagcc
    14701 cgggttgcca gggtggtggc gattgagcca ccctggcacg ttcaccggct taagcgttcc
    14761 atagtagcga ggaacatcaa gtgaacggaa cagccttcga tcttacatat tcagaatata
    14821 atggtgccgc cgctcgcgat gctgatggga tctatcgcgt catgtatatc gcaaaatttg
    14881 aagaagcggt ttacgtattg cattgttttc aaaagaaaac acagactacc agtcaatcgg
    14941 atgttgattt agccaaacga cgctataaag aacttgttca ggagcggaaa aatgaaaatt
    15001 gaaacctttg atagtgtgtg ggatgcggtg agtgacaccc ctgaacaagc ggaaaatatg
    15061 cggatccgtg ccgaacttgt aacgatcatt aataactgga ttgaacaaca agggtttagc
    15121 caggctcagg cagcgtctgc tttaggggtc actcaacctc gcatatctga acttgccaga
    15181 ggtaagatcc agatctttag tatcgataaa ttgatcacca tgatggctca tgcaggattg
    15241 catattcaac gtattgaaat tcaatatcca catgctgctt agtgctataa atacagatgg
    15301 cgacaattga cgccgccatc tgcataactt gtcttacatc ttctccagca acttcttcac
    15361 atctttgcca ttgcgggaat ctttattccg ctcggcccag tcattcaggc gacgtttcgc
    15421 ttcgtcctgt agatgtttgc gcagtaactg atccacttgc aggctgtaat tgagttgctg
    15481 ccagttgcca taaacccgca gcggtactgg cgtttctttg aggaaatcaa tcagtttgct
    15541 ttccccgttc caaccaccaa tcacacgaat gtcaaactgg gtgtcgcagg tttgatctgc
    15601 cagattcaac atgccttccc ctgtcagcgc cagcactggc gattgacctt gcatgtcgtt
    15661 taacgtcacg acgccatcct tcaacgtcaa atcggtggta aagcggtcaa gacgcgttac
    15721 gttatcgaag ttttcagcgg ccttcacatc accaccatta cgttctaccg cttgctgaat
    15781 catctgctgg aagttcatcc cttccatgcg cgtgtcggtc atttcgacat gtgcctgtcc
    15841 ttgccagtta tggcggaatg cgtcggcatc tatgtcagca ccggagaaat caccagccag
    15901 tgacatcttt ccggtcaacg aaatcggata gttaaacgcc ttcagaatgg tgccaatctc
    15961 aacgttttcc agccgtggct ggaagtttat ccgcggattt attgatgtcg cgtccagcgt
    16021 gcccggcagt gaaacctgtc caccgttaag tttgccctgc agttgagtaa tttccagcaa
    16081 acccgacttg ttggtcattt gcgtggcaac atctgtaaaa ttcattccgc gccagcgcac
    16141 gttactggcc tgcaacaaaa tatcagccgt aaagccttgc agtccctgat aggccggttc
    16201 atcaatacgc gaagaaatga ccgggcgcgg caacgttgat tggctctgcc cttgctgcgc
    16261 ggcaccgttc tcaccgttcg ctgtttcatt aagggggatg aggttgtcaa gattcagttg
    16321 cgggaattgc agtctcagct gccattccgg tttctctgtc aacgtgacct gtgcttgccc
    16381 gctcagcgta ctgtcattgg cggtcaaact aatttggtta aatgaaaggc gtttatgtga
    16441 ctcctgccac tgggcctgga aactcccctg cccctgaata ccttgttttg gtaaatcggc
    16501 accctgcaac tgccagttaa tttgttcaat agccgccgtt aaatcatgcg gataatcaga
    16561 agcatctacc gtaccgttaa gggatatcgt gagatcgcgc tgatcgcgat taacccgccc
    16621 ggagaactca aatgagccac gatgttgggg atcttgttcc atttgcaggc gaatattgcg
    16681 gattgtcacc tgctcgtcat cttcatgctg gaacaccagc acgctgtccg ccaccttaag
    16741 actggatata tcaaacgacc atccgcgatc gtctgacaga tccggcaagg tattgtcgcg
    16801 cggtgcaacc ggagcgtctt cactgcgcac cgcttccgtc tgcggcgtca gttgaatcac
    16861 tgcccctttt agcatcacct gcttaacgct cagttgatga ctcagtagtg gtaaaagcgc
    16921 cacgtccaga cgcatgttgt cggcgcgaac cagtggctgg ctcgcgccct gggcggtgag
    16981 agacattcgc ccggagagga tactaagctg cggccagacg tgccaacgca gtggcccgtc
    17041 gagctgcaat tgataaccgc tacgtgcagc aacttgcttg accatatagt cgcggaaatc
    17101 attcggattc accagcaaca ctaacgcaga taacccggcc accagcacga ccaggagtat
    17161 catcagcgtc gtcagaaatc gtctcatggt atcctcaatg ggcctgagtt agtctttatc
    17221 gattcggctg gctaccgcgc cctgctggtt gcgatatttc gcatcttcac ggcggttgta
    17281 aggtcgcgcc gccgggccgg aaagcggctc aaagctcagc gcaccaatta acatgcccgg
    17341 acgcagcgcc agcggcagct taccggagtt gtagaactcc agcacaatgc agccagacca
    17401 gcccggatcg atgcggtgcg cggtgacgtg caccatcagc cccagacgcg ccagtgagga
    17461 acgcccgtcc agccagccca ccagatcggc tggcagcgtc accgactcca gcgtcaccgc
    17521 cagcgccagc tctcctgggt gaagatagaa cgcctcgctt tcatcgagaa cgatctcatc
    17581 gctcatcacg cggtcaagcg cggcgctcac ttcatctttg ggaccgctca gatcgataaa
    17641 cgctgccgtg tgaccacgga aggtacgaaa tttattgccc aggcgcacat ccaccgtcgc
    17701 gccattaata cgctccactg gcggacgtgg gttgatcgac aaacggcctt catcaagcca
    17761 ggcttcaata tctcggtcac acagacgcat ggcattttct cctttcgtgc atcactccct
    17821 taacgccaat tacgtcaagg gcataccagg ttatcactga acggtacaca atttatcaag
    17881 cttattcaaa gaactgactt attttcgctt tcaatatatc gatggcgatg cggtttttcc
    17941 cgccgcgcgg cacgataatg tccgcatatt gtttagaagg ctcaatgaat tgcaggaaca
    18001 tcggacgcac ggttttttga tattgcgcca tcactgaatc cattgaacgc ccacgctcgt
    18061 taacgtcacg cttgatgcgg cgcatcaggc agatatccag cggggtatca acgaaaatgg
    18121 agaagttaag ttcgtcacgc aaacgcgcat ccgtcagcag caaaatgcct tcgagaatga
    18181 tgactttctt cggctcaacc gtcaccgttt ctttcatacg cgtatgttca acatagctgt
    18241 aaaccggcag gtcaatggcc gagccgcgtt tcaacgcttg taaatgctca agcaacaggc
    18301 tgtgatccat cgcgctggga tggtcgtagt tggtcttaac gcgttcttcc atcgacagat
    18361 ggctttgatc tttgtaatag cagtcttcgg gaattacgcc gatgtgttca tcaccgactt
    18421 gctcacgtaa ttcacgataa agggtactgg caataagact cttgccggaa gccgatgcgc
    18481 cagcgatacc gataatgacg cactgatgag actgatcagt catatattta gcgacctgat
    18541 taacctggat gttaggaagg gggcgacgaa gcgccaaacg cggcaattat agggatttca
    18601 tccgcctgat accagtcgaa tagcgttgcc gcgcgctcag agttaattgt tgacgaagaa
    18661 ttcccggtgg caaattacgt tgatcagttt tatacaaagt aaaaattgtt atacgcagtt
    18721 gcgcaaatta tccgccttta cgtcacttta tgagcaattc gcatataaaa tgtaaaactt
    18781 ttgtactagc ataaacacag aaacgaatac tggcgacctg gtcttgcgga taaagcggta
    18841 atgagcaaac aatcacagca tgtattaatt gccctgcccc acccgctgct tcacctggtc
    18901 agtttaggtt tagtctcgtt tatctttacc cttttctcgc ttgagctttc gcagtttggc
    18961 acccaactcg ccccactgtg gttcccgacg tccatcatga tggtggcgtt ttatcgccat
    19021 gccgggcgca tgtggccggg gattgcgctg agctgctcgc tgggaaatat cgccgcatcc
    19081 atcctgcttt tttccaccag ctcgctgaac atgacctgga cgaccatcaa tattgttgaa
    19141 gccgtggtcg gggcagtgct actgcgtaaa ttgctgccgt ggtataaccc attgcaaaat
    19201 ctggctgact ggctgcgtct ggcactcggc agcgccattg ttccacctct gttagggggt
    19261 gttctggtta tcctgctgac gcccggagac gatcctctca gggcattttt gatatgggta
    19321 ctgtcagaat ccatcggcgc actggcactg gtgccgctgg gattgttatt taaaccacac
    19381 tatctgctgc gccatcgcaa cccacggttg ctttttgagt cgctgctcac gttagccatc
    19441 acactgacgt taagctggct ttcgatgctg tatctgccgt ggccttttac tttcattatt
    19501 gtgctgttga tgtggagcgc cgtgcgcctg ccacgaatgg aagccttttt gatcttcctt
    19561 accacggtga tgatggtgtc actgatgatg gccgctgatc cctccctgct tgctacgccg
    19621 cgtacatacc tgatgagcca tatgccgtgg ctaccgtttt tgctgatcct gctgcccgcc
    19681 aacatcatga ccatggtgat gtatgccttt cgtgcggaac gcaaacacat ttccgaaagc
    19741 gaaacccgtt ttcgtaacgc catggaatat tccgccatcg gcatggcatt agtgggcacc
    19801 gaggggcaat ggctgcaatc caacaaagca ctctgccagt ttctcggtta cagtcaggaa
    19861 gagctgcgcg gactcacctt tcagcaactg acctggccgg aggatctcaa taaagatctc
    19921 caacaggttg aaaagctgat cagcggtgaa ataaacacct attcaatgga aaaacgctac
    19981 tacaaccgca atggcgatgt tgtctgggcg ttgcttgccg tctcactggt gcgccacacg
    20041 gatggcacgc cgctctattt tatcgctcag attgaagaca ttaacgagct aaaacgcacc
    20101 gaacaggtga atcagcaact gatggagcgc atcacgctgg ccaacgaagc gggcgggatt
    20161 ggcatctggg agtgggagct gaagccgaat atttttagct gggataagcg gatgttcgag
    20221 ctgtatgaaa ttcctccgca tatcaaaccg aactggcagg tgtggtacga gtgcgtgctg
    20281 ccggaagatc gccagcacgc cgaaaaagtg attcgtgatt cgttgcaatc acgctcgccc
    20341 tttaaactgg aatttcgcat taccgtgaaa gacggcattc gccatatccg cgccctcgct
    20401 aaccgggtac tgaataaaga aggcgaagtc gaacgcctgc tcggcattaa tatggatatg
    20461 accgaagtga aacagcttaa cgaggcattg tttcaggaaa aagagcgcct gcacataacg
    20521 ctggattcca tcggcgaagc cgtggtctgt attgatatgg caatgaaaat tacctttatg
    20581 aatccggtgg cggagaagat gagcggctgg acgcaggaag aagcgttagg tgttccgctc
    20641 ctgacggtgt tgcatattac ttttggcgac aacggaccat taatggagaa catttacagt
    20701 gccgacacct cacgttccgc gattgaacaa gatgtggtgt tgcactgccg aagcggcggc
    20761 agctacgacg tgcattacag tattacgccg ttaagtactc tggacggcag caatattggt
    20821 tcggttctgg tgattcagga cgtcaccgaa tcgcgcaaaa tgctgcgcca gctgagctac
    20881 agcgcctccc atgatgcact gacgcatctc gccaatcgcg ccagttttga gaagcaacta
    20941 cgcatcctgc tgcaaacggt aaacagtacg catcagcgac atgccctggt gtttatcgat
    21001 cttgatcgct ttaaagcggt gaatgacagc gccgggcacg ccgctggcga cgctttactg
    21061 cgcgaactgg cgtcattgat gctgagtatg ctgcgctcca gcgacgtgct ggcgcgactc
    21121 ggtggcgatg aatttggtct gctgctgcca gattgtaatg tcgaaagtgc gcgttttatc
    21181 gctacacgca ttatcagcgc cgtgaatgac tatcacttta tctgggaagg acgagtacat
    21241 cgggtaggtg ccagtgccgg gattaccttg attgatgaca acaatcatca ggcggctgaa
    21301 gtgatgtcgc aggctgatat cgcctgttat gcctccaaaa atggtggacg gggccgggtg
    21361 acggtttacg aaccgcagca agctgccaca aatagcgaac gggcggtgat gtcgcttgat
    21421 gaacagtggc ggatgattaa agagaatcag ttgatgatga tcgcccacgg tgtcgcttcg
    21481 ccgcggatcc cgcaagcgcg taatttgtgg ctgatttcac ttaagctctg gagttgcgaa
    21541 ggcgagatta ttgatgaaca aacatttcgt cgtagcttca gcgatccggc acttagccat
    21601 gctcttgacc gacgggtatt ccacgatttt ttccagcaga ccgcaaaagc gattgccagt
    21661 aaaggcttaa gcatcgccct ccccctttcc gttgccggtt tgagtagcgc cacgctggtg
    21721 aatgaactaa ttgagcagct ggaaaatagc cctctaccac cacggttatt acatctgatt
    21781 attccggcag acgcgatttt agatcacgca gaaagcgtgc aaaaactgcg gctggcggga
    21841 tgtcggatcg tattcagtca ggtgggccgc gatctgcaaa tcttcaactc gttgaaagca
    21901 aatatggcag attacctgct acttgatggt gagttatgcg ccaacgtgca gggaaatttg
    21961 atggatgaga tgctgattac gatcattcag gggcacgctc agcgactcgg gatgaaaacc
    22021 atcgccgggc cagtcgtttt acccttagtg atggatacgc tttctggcat cggcgtcgat
    22081 ctgatttatg gcgatgtgat tgccgatgcc caaccgctgg atttgctggt gaatagcagt
    22141 tatttcgcga ttaactgagg gagcgtttgc ccattgcctg atgcgacgct aacgcgtctt
    22201 atcatgccta caaatcgctc attccccagg ccggataagg cgctcgtacc gcatccggcg
    22261 accaacgtca tgctccgtct ggttgccagc cttccgtata ccagatatgc aacagcgcat
    22321 aagaacgcca gggcttccag cgctcggcat atcggcggat ttgtgccggt gtcattcccg
    22381 gaaatcgctg tttaatcaga taatcatccg gtagaaaaac atctttcgcc tgccagccgc
    22441 gcaaggcgaa ataattcgcc gtccagcgcc cgatacccgg aaaagtttgc agcgttttca
    22501 tcgcttgttc tacgtcgctc ggtattgtca ttggtaaggt gccctccagc gtcgcatttg
    22561 ccagatgaat cagcgcctcc gcccgtttca acggcatacc taacgctttt aatgcctgcg
    22621 ggtcggctgc tgccagccgc tgaggcgtgg ggaagcagac ataatccgga aaatcatcca
    22681 gccgttcgcc ataaagctgt gccactctgg cggtcaattt tgccgccatc gccacgctca
    22741 ccagttggcc taaaatcgcc cgcacgccct gctcaaaagc atcaacacaa ccaggtaaac
    22801 gcaatccggg ccgcgccgcg cctaacttgc ccaacgcacc gttaacaatc tgcgggttac
    22861 attgcaaatc aaacaggcgg ctcattttcg ccagacactc agcggcaaca ggttctaaac
    22921 ctgcacttaa atttatgtgc agagtatggc tggctatatc cggaatagca gtcaccacgc
    22981 cgcgatattc gcccaccgcc agactacggg cataataact gtccgcgacc gtttccacac
    23041 cgttcaccgc acgggcggcg agaaatccca acatccacga ccagtcatac ggcggctgcc
    23101 agttcagggt atacatcgca tctccttatt catccgcttt cagcataaac gttattcaga
    23161 cgctacgctt tgctttcata ttccggttgt cgcgacggca acatttcgct aaagtcacgc
    23221 cccttcttca ccggcatggg gattatttcg tgtttattgg ttttgattac ggtacagcaa
    23281 actgttcagt ggcggtcatg cgcgacggta agccgcactt gctaaaaatg gaaaacgaca
    23341 gcacgctgct gccttcaatg ctttgcgcgc caacgcgtga agcggtaagc gaatggctgt
    23401 accgccatca cgacgttccg gcagacgacg atgaaacgca ggcgctgtta cgtcgggcga
    23461 ttcgttataa ccgcgaagaa gatatcgatg ttacggcgaa aagcgtgcag ttcggtcttt
    23521 cctcactggc acagtacatt gatgatccag aagaggtgtg gtttgtgaaa tcaccaaaat
    23581 cgttcctcgg tgccagcggc ttaaaaccgc agcaggtagc gctgtttgag gatctggtct
    23641 gcgcaatgat gctgcacatt cgccaacagg cgcaggcgca attgccagaa gcgattaccc
    23701 aggcggtgat tggtcgcccg atcaacttcc aggggctggg cggcgatgaa gcaaacgccc
    23761 aggcacaagg gattctggag cgcgcagcga agcgtgccgg gtttaaggac gtggtattcc
    23821 agtacgagcc ggtggcggct gggctggatt acgaagccac cttgcaggaa gaaaaacggg
    23881 tgctggtggt ggatattggt ggtggtacga ctgactgttc attgctgctg atggggccgc
    23941 agtggcgttc gcgtctcgat cgtgaagcca gcctgctggg tcacagtggt tgccgtattg
    24001 gcggtaacga tctggatatc gcactggcgt ttaaaaacct gatgccgtta ctgggcatgg
    24061 gtggcgaaac cgaaaaaggc atcgccctgc cgattctgcc gtggtggaat gcggttgcca
    24121 tcaacgacgt ccctgcgcaa agtgatttct acagtagtgc caacggtcgt ctgcttaacg
    24181 atctggtacg cgatgcccgc gaaccggaaa aagtggccct gttacagaaa gtctggcgtc
    24241 agcgtttaag ctatcgcctg gtacgtagcg cagaagagag caaaattgcg ctttcaagcg
    24301 tagcggaaac ccgcgcctca ctgccgttta tcagcgatga actggcaacg ctgattagcc
    24361 agcaagggct ggaaaacgcc ctcagtcagc cgctggcgcg aattctggaa caggtgcaac
    24421 tggcgctgga taacgcccag gaaaaaccgg atgtcatcta tctgactggt ggtagcgccc
    24481 gatcgccgct gattaaaaaa gcgctggcag aacagttgcc gggcattccg attgcaggcg
    24541 gcgatgactt tggctccgtc accgccgggc tggcacgctg ggcggaagtg gtgtttcgtt
    24601 aattgaaaaa tggctcagca gcggcttaaa tcagcctgcg cctgagccag tttttctgcc
    24661 gcctgacgta gcggctccat caacggcatg gtccgtgctg gagcatgaag gcggaatcgc
    24721 tgcaactctg tcgcaccgac agtcaacgcc gattccagcc gatgtcgttt ggcggccatt
    24781 ttagtcagta ccgcttgtct gtctgcaggc gttaccgctt cgttcggcct gaaaacaaaa
    24841 cggcgttcgc aactcgcttt ccagtcgata accgcctggg tcagatgatc accaaaacct
    24901 tttacttgct taacgctccg tcgggtaacg tctgccgccg tttcaatacc aaaggaccgt
    24961 aacgccgctt tacgcgcagg gccaacgccg ggaatagagg caacatcaat aaaaaatccc
    25021 tccagaaact tctgcttctg ccgttccctt gcggtgtcct gaagtgctgc cagatcgcgc
    25081 ttttcttctt ccggtaaccc aagaatttcg tccttcattt tttcgagcat cgtccgtttg
    25141 gcgataaaac cttccagccc gcccaactgt tggatctggc tgactaaatg gtcatagtcc
    25201 atttttgcgc gattaaaagc ctctcttcgt tgctggattt ctgctttgta cgctttgctt
    25261 gtcaggctgc caataatcca gatagccgac agcacaggaa ccaaaataat ataacgtggt
    25321 tctgtctgaa ggccgcaaag caacagcgat aacgctgaca gtgcgatctc aatcagaatg
    25381 atgtattcac gccttaacag gcctaaagga agcggcctgc cagtcggttg gaaatgatcg
    25441 ggtaatggca gttgcaatgc tggcggtgct actgacgcca tcaccatcgc ccagactttc
    25501 gccagcacaa aatcaccgct ggtggtaatg acctcttcgc cgagatcaat aaaatagata
    25561 acgccttgat tatccagagc acaccacggg cagtcagcca aatgaccggg ataaacatgc
    25621 attgccgaaa cggtacattt ctttaattgt tggcgtagag aatcaagtgc cgctacccac
    25681 gcttttgccg tcggacgccc ggttgccacg ccactttccg tgaatgcctg ctgaaacatg
    25741 gcttcaacat cgcccggtaa catcgacagc ggaatagatc gtggcggcgg ttttaaacca
    25801 cgtcgctgat tatctgacgc gtaggcataa cggaaatggg caatatccgt ctccagcgca
    25861 ttacccgcat cagagataag cggtacaccg gaataaggat gccgaccacc aaacaagacg
    25921 tgaaaaatca gcaacgcaag gccaaaatta tcgtgattcg cggtgcgttc aaagccaaca
    25981 aatgacgaca aggtttgcag ctctggcgac gtaaaatgcg acacgcccac ttcgcataaa
    26041 tgcagcgttc cattggcgtt aatctgaaag gagtcgctat caatcaacac cactttgctg
    26101 tcacggccta ccataaagct gttctggttt acgtcaccca caacgtgccc gtgctcgtga
    26161 accgtagcaa aagatgaagc aatattgcgc gcaacataga ggagaaaatc ccacgcacaa
    26221 tgagggtaac tctgacgacg atgtgccggg ctatagatca tatgaatcgg ttctttacca
    26281 gaaacttttg gcatcataaa accgattact tttccgcctc gtccaccgtg aagcgttgcc
    26341 tgcggccagg cgacataatt caacaattgc gcgtcagctg tcgcagccat aaaggcaagt
    26401 ttgtcctgtt ttaaggcggg tggcggcgtg tgataaatct tggcgacgct atcgacaaac
    26461 tcctcgatat cataaaccgc accttcgccg cctttgccca gttcacgccc gagagtggtc
    26521 aattcatctg tcgatgtaaa tacttttata tttgtcttca taatgtttca agggcagtca
    26581 tattatattt caatgaataa taacactgcc cattataatt atttctatat tggaagagtc
    26641 ccactcaaga ataaaaatga taatgcaatt tcatcaggat acaattcaat tccaacctga
    26701 taatgattca cttccacagg ttttttgcta acttttacta cacagggatt ctggagaaaa
    26761 aaatgaaaaa gatattactt atgttgagtc tgctattttt tactaccgca agctattgtg
    26821 agatttcgga tacgttagtg acaggaggtt atgataaaaa ggcgatgtct gacgcgatta
    26881 aacatgcgcg gaaagaaacc gatcaattca tcgaagttat gaataaaaaa gatgctgata
    26941 ctttcgcagt caaagccccg ataaccgatc gcggagaaac cgagcatttc tggctcacgg
    27001 atgtcactta ttctaacggc atgtttattg gtataataag caatgaccct ggtattgtta
    27061 caaacgtaga atatggccag gagtggaaaa tcaagaaaga agatatttca gactggatgt
    27121 atacgcgcgg ggataaaatt tacggtggtt ataccattga tcctttattg gtcacctatc
    27181 caaaagaaga agccgacgag ctaagagcaa aattagttcg ctagccagcc attatattga
    27241 ttgcttaaac tggcggttga accgttgtct tcaaccgcca tgcgatgttc attctgtcca
    27301 catagccagt gccaacgttt tatcatcatc cgtacgctcg tttaccgccg ggctggacaa
    27361 aaactgcttt aacaattcag gtaataaatc gagttgttcc tgcgttgctg acgccagtcc
    27421 attgaaaaac ggggtaaaga aaggcacatg cggagaatta tccagcatat ttaacgccag
    27481 tcgctgaata ccatccgtaa atgctgcaac tttatgcgca cgctcagtac tggtgtaaat
    27541 ttccagtctg gaaacagcat cgtcatcggt aatgaagtgc gtcatattgg catattcccc
    27601 caccatcggc gtgagcggca gttgcaaacc gtgacccaaa tctaccacca cgccgccatc
    27661 gccaatctgc ataatcaagg tgccattagc tgatgaaatc aatcccaaaa atgtgcaggc
    27721 aaaatcgcga accgctaatt ctttggcttc cgcctcggca aaa
//
LOCUS       DAMZFT010000060        27048 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.060, whole genome shotgun sequence.
ACCESSION   DAMZFT010000060 DAMZFT010000000
VERSION     DAMZFT010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 27048)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27048)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27048
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.060"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            59..1231
                     /gene="iadA"
                     /locus_tag="PN977_002636"
     CDS             59..1231
                     /gene="iadA"
                     /locus_tag="PN977_002636"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HDI6042655.1"
                     /translation="MIDYTAAGFTLLQGAHLYAPEDRGVCDVLVANGKIIAVASNIPS
                     DIVPDCTVVDLSGQILCPGFIDQHVHLIGGGGEAGPTTRTPEVALSRLTEAGVTSVVG
                     LLGTDSISRHPESLLAKTRALNEEGISAWMLTGAYHVPSRTITGSVEKDVAIIDRVIG
                     VKCAISDHRSAAPDVYHLANMAAESRVGGLLGGKPGVTVFHMGDSKKALQPVYDLLEN
                     CDVPISKLLPTHVNRNVPLFEQALEFAHKGGTIDITSSIDEPVAPAEGIARAVQAGIP
                     LARVTLSSDGNGSQPFFDDEGNLTHIGVAGFETLLETVQVLVKDYDFSISDALRPLTS
                     SVAGFLNLTGKGEILPGNDADLLVMTPELRIEQVYARGKLMVKDGKACVKGTFETA"
     gene            1296..2207
                     /gene="hypT"
                     /locus_tag="PN977_002637"
                     /gene_synonym="qseD"
     CDS             1296..2207
                     /gene="hypT"
                     /locus_tag="PN977_002637"
                     /gene_synonym="qseD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypochlorite stress DNA-binding transcriptional
                     regulator HypT"
                     /protein_id="HDI6042656.1"
                     /translation="MDGCGAVLHNIETKWLYDFLTLEKCRNFSQAAVSRNVSQPAFSR
                     RIRALEQAIGVELFNRQVTPLQLSEQGKIFHSQIRHLLQQLESNLAELRGGSDYAQRK
                     IKIAAAHSLSLGLLPSIISQMPPLFTWAIEAIDVDEAVDKLREGQSDCIFSFHDEDLL
                     EAPFDHIRLFESQLFPVCASDEHGEALFNLAQPHFPLLNYSRNSYMGRLINRTLTRHS
                     ELSFSTFFVSSMSELLKQVALDGCGIAWLPEYAIQQEIRSGQLVVLNRDELVIPIQAY
                     AYRMNTRMNPVAERFWRELRELEIVLS"
     gene            complement(2294..3697)
                     /gene="nhaC"
                     /locus_tag="PN977_002638"
     CDS             complement(2294..3697)
                     /gene="nhaC"
                     /locus_tag="PN977_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001208425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HDI6042657.1"
                     /translation="MREKPSFYVALTPIIFMMIVLVVGIGVMGWPASVCLLISAAFSC
                     IIAMAKLKYTWDEIQGFIIDKISAVMAPVLIVIFVGFMVATWSYAGTLPMLVYYGMLL
                     VAPAWLYAIAFFLNAVLSYVSGASWGSVASIGVALMGIGSGLHADLPILAAAIVTGAY
                     FGDKLSPLSDTTNLTSAVTKTKLYDLIKYLLWTTIPSTIISLLFFTWLGMIHTSTGSI
                     SPESMQMLVQLKELYKFGLLPLLPMLIVLCFTFLRLPTVPALLSSAFVAVLVGWLYQG
                     FPLAEGIKATMSGFRLTMITDSTTLLPSVKELLEQGGLNNQGGFLSFIICAMMFAGIL
                     TGTGMMDVTLQSAANKIKSAFGAILASGVLAIIINLLTGSDGLNKIVISELMMKKFED
                     LNLSPLVLARTLEDFGTMSAPIIPWSAAGLYMATTLGVPTFSYLPYCVFCFCSMIFAL
                     IYASTGFRLLRFNEAKA"
     gene            complement(3712..5082)
                     /gene="ibeA"
                     /locus_tag="PN977_002639"
     CDS             complement(3712..5082)
                     /gene="ibeA"
                     /locus_tag="PN977_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000397940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative intracellular survival FAD-dependent
                     oxidoreductase IbeA"
                     /protein_id="HDI6042658.1"
                     /translation="MEFYLEPARNIPVLATTEVLVVGGGPSGIAAAMSAAREGAATML
                     IERFGCFGGMMTTAGVESIAWWRHENTVESGGLAREIEETAKSMGASSPEPQSNSQAI
                     NAERFKLVADAMLEQAGVRRVLHITAVDVIKQGNNLLGVITESKSGRQAILANVIIDC
                     TGDADIAWFAGAPFIKREREELMCMTTVFSCANINKNAFMQNIKSTEPKYGDWGADEE
                     NKNWSYDVHESCRDMFSPYLGKVFAKGKSAGIIPKDVTLGGSWSTVTEYGDANYLNVV
                     SIPAVDCTDVFDLTRAEIEGRKQAMQAIEALRQFQPGFEQAQLKNFGMTVGTRESRHI
                     IGRVQLTENDICNEGRHADSIGVFPEFIDGNGHLKLPLEANYFQIPYGVMIPQQVENL
                     LVCGRAIDADNFAYATIRNMGCCIVTGEGAGTAAAIAIKNNTTVSQVDIQTVQERLQQ
                     NGVKVF"
     gene            complement(5369..7303)
                     /gene="dhaR"
                     /locus_tag="PN977_002640"
     CDS             complement(5369..7303)
                     /gene="dhaR"
                     /locus_tag="PN977_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001445381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase operon
                     transcriptional regulator DhaR"
                     /protein_id="HDI6042659.1"
                     /translation="MNKESYHNDLKNKWKMFVKHGWVATNSTNHVMLRSWQKCLKHCD
                     PRHWNTPVKASGQTLQTIFSRNEEFIRISQRVVEDHFTLAGDDRLAFLIIDPHGWVLS
                     LNAAGDYSSQLRELGIESGMSWAEDGIGTNVYSLCRETNLYTQLEGAEHFSEQLHCYA
                     MSAAPVIDYYGNIHGYIVCIIETTAELVKLKTSYSCATEIANYIYIENEQKLINKVLC
                     QHNAVIECMDDGFICWNSHSLITMVNSQAQTLLNIDKESLIGQNIRKGFVFPPILNEA
                     ITQRNKLSQKQIVLECRGEFIELMVTLRPLSDGSFLLFLHPLDKIRKIAQQQISTNAN
                     FTFDSLHAASGGMKQVLLIARRAIKSISPILINGEEGVGKLSLAMAIHNESEQRDGPF
                     ISVDCQMLSPENILHELLGSDVGPSPSKFELAHNGTLYLDKVEYLSGEVQSVLLKVLK
                     TGLVTRSDSHRLIPVRFRLITCTSSSLREYVQQGAFSRQLYYEISMNEIEIPPLRKRR
                     EDLKQMIDDIIDKYQERTRKKMTITPDANSVLLEYRWPGNISEFKNRMEKVFINCNRL
                     VLGLENIPLDIRQNNSSGDDDIPHLTSLAELEMQAIEHTCRVCEWNLTKAAEVLKIGR
                     TTLWRKLKIYNLYPNVEHAD"
     gene            7697..9436
                     /gene="gcl"
                     /locus_tag="PN977_002641"
     CDS             7697..9436
                     /gene="gcl"
                     /locus_tag="PN977_002641"
                     /EC_number="4.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001357513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate carboligase"
                     /protein_id="HDI6042660.1"
                     /translation="MARMRAIEAAVEILKKEGISVAFGVPGAAINPLYAAMKKSGGID
                     HILARHVEGASHMAEGYTRSQNGNIGVCIGTSGPAGTDMITGLYSASADSIPILCITG
                     QAPVGKLHKEDFQAVDIEAIATPVTKMARTILEAGQLPGIFQKAFWEMRSGRPGPVLL
                     DLPFDVQMTEIEFDIDLYQPLTPWQPQATRAQAVRALEMLNGAEKPVIIAGGGIINAE
                     ASELLREFVELTGVPVIQTLRGWGALSDDHPLMIGRMGCQAGHRYGNASYLASDFVFG
                     IGNRWANRHTGAIETYTEGRKFIHVDIEPAQIGRIFAPDLGIVSDAESALTLFIQVAR
                     DMKSRGELKDRSRWIAECAERKRTMLRRSDFDCNPIKPQRVYHEMNKVFGPETRYIST
                     IGLAQIAANQFLHVYRPRHWINACQAGPLGWTMPAALGAVKADPSVPVVAISGDYDFQ
                     FLIEELAVGAQFNLPYIHILLNNAYLGLIRQSQRAFDIDYCVQLSFENINAPEINGYG
                     VDHKAVVEGLGCKAIRVFASQDIAPALQEAQRLRDEFHVPVVVEIIAERVTNIAMGPD
                     INKVTEFEEILDL"
     gene            9449..10231
                     /gene="hyi"
                     /locus_tag="PN977_002642"
     CDS             9449..10231
                     /gene="hyi"
                     /locus_tag="PN977_002642"
                     /EC_number="5.3.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001682525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxypyruvate isomerase"
                     /protein_id="HDI6042661.1"
                     /translation="MAKFAANLSMLFTEYPFIERFAQASHAGFHGVEYLFPYDFSADE
                     LVSQLHQHNLTQVLFNLPAGNWQEGERGIACHPGRAKEFQESVSRAIEYALALNCSQV
                     NCLAGKHPGGYSHEQCHETLVENLRYAVDKLASHGIKLVLEAINTKDIPGFFVNNTRQ
                     ALNIIHDVKHPDLRYQYDIYHMQIMEGNIATTIKNNLNNIGHIQLADNPGRHEPGTGE
                     INYPWLLNYIDQIGYQGWIGCEYIPSTTTTESLRWLKNETQF"
     gene            10249..11136
                     /locus_tag="PN977_002643"
     CDS             10249..11136
                     /locus_tag="PN977_002643"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001020468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HDI6042662.1"
                     /translation="MNIGFIGTGIMGKPMAYNLQQAGHTLYFSAHFEPAPQELIGERG
                     IVCSTPREVAQECEVIITMLPDTPHVEDVLFHPNYGVIHGLSHGKIVIDMSSISPVAT
                     KAFAQRIIAGGAEYIDAPVSGGEVGAKAGTLSIMVGGCEEVYLQIKPILELMGKNITL
                     VGNVGDGQTCKVANQIIVALNIEAVAEALLFASKSGADPARVREALMGGFASSRVLEV
                     HGERMIKGTFAPGFRISLHQKDLNLALENARLLNTPLPNTAMTQQLFSACAALGGKEW
                     DHSALIRALETQANFTIRK"
     gene            11211..12353
                     /locus_tag="PN977_002644"
     CDS             11211..12353
                     /locus_tag="PN977_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001523874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HDI6042663.1"
                     /translation="MKIVISPDSFKECLPAWKVAEALATGWRKVLPGSQLVCLPVADG
                     GEGTLETLIHATDGTFYTKKVTGPLGESIHAQYGILGNQTTAVIELAQASGLELVSPV
                     QRSPLYTTSFGTGELILAALEHNIDTVILCLGGSATNDGGIGLMSALGASFTDAEGLS
                     VSVNGMGLAAIHHIDLQHLDPRLKNVKFIAACDVTNPLTGDNGATRVFAQQKGASADN
                     LEQLEQGMKNYARCIYRCCGKEVDTIPGSGAAGGVGAALMAFLDARLQPGISLVLEAI
                     QYTQHLKYAALAIVGEGKLDRQSLNGKAPVGAAKIAQMMGVPVIAIAGYIDDQLDLNE
                     LRQCGIEACFSVVNGPCDLPTALSQGENNLIRLGENLAGYFHAVLS"
     gene            complement(12409..13950)
                     /locus_tag="PN977_002645"
     CDS             complement(12409..13950)
                     /locus_tag="PN977_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BCCT family transporter"
                     /protein_id="HDI6042664.1"
                     /translation="MQYLKKRFSLIELNVFIPAILFIAVIILCLTIYPQDTSRYINKI
                     HHFLTWEMGGIFLVMTFLVVLCCLWLAFSRYGDILLGQSGEKPDFSLLTWLGLIFTSG
                     TGGSLLYLASVEWIWIIQQPPFGATAGSAQAARWASAYGMFHWGPSAWAWYLICAVPI
                     GWFMHVKKTNSLKVSDLCRGCLGARADGFCGHCVNFFYMFGLLGGAVTSLALGTPMIS
                     AVFCHVFHLDPAGQFINVMVIFIWTLVPLLILFFGLKKGVAWASNWNIRADIFMLLAI
                     LICGPTAFILNQSIDGFGLMLQNFVAMSLSTDAIGRSGFPQMWTVFYFSWWVVYAIPF
                     GLFIARISKGRTIRQLIVCGTLAGSLGCMVFYMVLANFGLSLQTTHVIDFVPILNEQG
                     RGVVVSRLLEQLPASQVFLVAFGAIALISYITGHCTVGYALGFATQKRADSESEPAFW
                     NVAFWLIMTGIVAITLYLLDAQSLQPLQTVSILAGLPLCGVVFILLKSFLTQLAAEEK
                     TARDE"
     gene            complement(14251..15615)
                     /locus_tag="PN977_002646"
     CDS             complement(14251..15615)
                     /locus_tag="PN977_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000228116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HDI6042665.1"
                     /translation="MVDMINESARQTPVIAQTDVLVIGGGPAGLTAAIAAGRLGARTM
                     IVERYGSLGGVLTQVGVESFAWYRHPGTEDCEGICREYEGRARALGFTRPEPQSISEV
                     IDTEGFKVVADQMITEAGVEPLYHSWVVDVIKEGDTLCGVIVENKSGRGAILAKRIVD
                     CTGDADIAARAGAPWTKRGKDQLMGVTVMFSCAGVDVARFNRFVAEELKPTYADWGKN
                     WTIQTTGKEDQMFSPYMEDIFTRAQQDGVIPGDAQAIAGTWSTFSESGEAFQMNMVYA
                     FGFDCTDVFDLTKAEIAGRQQALWAIDALRHYVPGFENVRLRNFGATLGTRESRLIEG
                     EIRIADDYVLNQGRCSDSVGIFPEFIDGSGYLILPTTGRFFQIPYGCLVPQKVENLLV
                     AGRCISAGVVAHTSMRNMMCCAVTGEAAGTAAVVSLQQHCTVRQVAIPDLQNTLQQQG
                     VRLA"
     gene            complement(15633..16901)
                     /locus_tag="PN977_002647"
     CDS             complement(15633..16901)
                     /locus_tag="PN977_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016249535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDI6042666.1"
                     /translation="MTSNWKRWITLALCGIAGSAIYKLPYLRETYYDAMQQATGATNA
                     ELGFLMTAYGLVNFLLYLPGGWAADRFSARKLMTFSLISTGISGFYYATFPSYTMICL
                     LHALWAVTTVFTFWAVCVRIIRTLGTSEEQGRLYGYWFLGKGLTSIVLGFLSVPVFAK
                     FGEGVDGLRATIIFYSVVTILAGVLAWFVCQDETHSEDKANFRLADMAFVLKMPTVWL
                     AGVVTFCMWSIYIGFGMVTPYLTQILHMGESEVAVASILRAYVLFAMGGLIGGQLADR
                     CASRTRFMIYAFIGMIVFTTVYFFLPGESRYVTIALANMVALGVFIYSANAVFFSIID
                     EIRIPAKVTGTAAGLISLLTYFPEIYCYTMVGNMVDRKPGIAGYQDVFLFMLVCAFIG
                     LIAALVLQRVNRKSKNQVNEITENNADCAS"
     gene            complement(16969..18051)
                     /locus_tag="PN977_002648"
     CDS             complement(16969..18051)
                     /locus_tag="PN977_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001357509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol dehydrogenase"
                     /protein_id="HDI6042667.1"
                     /translation="MDKIIISPAKYIQGNGSLDNIATYAASLGTEPLIIADEFVTGLV
                     GDRVSQSFARENIIADLDVFCGECSQNEISRIREKFNQRKYNVVIGIGGGKTLDTAKA
                     VAYYQKIPVVVVPTIASTDAPTSSLAVIYTPEGQFSEYLFFPKNPDMVIMDTGVISAA
                     PVRLLVAGMGDALSTWFEARANQASGKATMAGGASTLAALAIARLCYTTLLEDGYKAK
                     VAVEQGVSTKAVENIIEANTYLSGIGFESSGLAAAHAIHNGMTQLEECHHCYHGEKVA
                     FGVLVQLVLENAPQEEIETVLNFCHSVGLPTNLHMLGVKEINKDKLRAVAEAATAEGE
                     TIHNMPFVVTAQDVLCAILTAHHLGL"
     gene            18377..19441
                     /gene="dhaK"
                     /locus_tag="PN977_002649"
     CDS             18377..19441
                     /gene="dhaK"
                     /locus_tag="PN977_002649"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HDI6042668.1"
                     /translation="MKKLINQIDSVVTEQMEGLIATWPHLQANYAPRYVWCKQTDNAV
                     ALISGGGSGHEPLHAGFVGMGMLTGACPGEIFTSPTPDQMIECAKAVDNGSGVLFFIK
                     NYTGDILNFETAVEMLHEEGIAVGTVIIDDDVAVKDSLYTAGRRGVAGTVFVEKIVGA
                     AALQGYNLGQCEQLGKDVNNATRSFGIALSACTVPAAGKPSFELADNEIEFGVGIHGE
                     PGIERRTLQDLNTLIDSVIAQLLDNTPWRRTLRHWDRHAGGWIDASSMNESFDQNAEY
                     IVLINGLGSTPESELYGVARVFMCAAQRQGIKISRQLVGNYCTSLDMAGFSISLLKCT
                     PEFLQLWDAPVNTPALRWGC"
     gene            19454..20086
                     /gene="dhaL"
                     /locus_tag="PN977_002650"
     CDS             19454..20086
                     /gene="dhaL"
                     /locus_tag="PN977_002650"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000701650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase ADP-binding subunit
                     DhaL"
                     /protein_id="HDI6042669.1"
                     /translation="MKIQNKHVIAWLESCAAHLTEQQDFLTALDRDIGDADHGLNMNR
                     GFSAVKATLPDIERQHIGNILKNTGMKLLSSVGGASGPLYGTLFIRASAAVGARTELT
                     LEEWLACLEEGIAGVIARGKAEQGDKTLCDVWVPVLHEAKKNLQAGMSPSLLLNTMVQ
                     DAATAVDNTINMQAKKGRASYLGARSVGHQDPGATSSWLMIKAMQEGFAG"
     gene            20090..22471
                     /gene="ptsP"
                     /locus_tag="PN977_002651"
     CDS             20090..22471
                     /gene="ptsP"
                     /locus_tag="PN977_002651"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001519422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HDI6042670.1"
                     /translation="MVAIVIVSHSLRLAQGVEELALQMSGGDVPLAIAAGIDDPQNPI
                     GTDAIAIMSAIESVWSPDGVLVLMDMGSALLSTEMALELLSEEQRSAIYLLAAPVVEG
                     AMSAVTSAAAGLSVTEIIAEVDLALCAKQQQLTPSSTAGEVIPAISPINHSGEWQTFC
                     WTIRNPHGIHARPAASILKVSAQYSANIIVIKGDKRASTRSLNELAMLGVRCGDEIIF
                     QAEGTDASDALKAIETLAKNHFGEAHLLASSEIPLTETPVVSAPVDGAISGLSVQNGI
                     AIGPVKWFTCERPEITQRTVDSPQEELSRIESAIDIVVCELADKAAGPEGDIFAAHKM
                     MLEDPKINRQLQQRLAKGKQAEFAWLEVMQALAEQYCQAETLYLREREADIRDLTRQV
                     LNQLCGGSEQHFITTAPCILLANDLLPSQITSLNKAHILGICLHNGGTTSHTAILARA
                     MGIPAIVKAAITPQNVRDNDTVILDGETGRLWLQPDEVTRLDLLQRAEAWRQQRDRQL
                     ADAMQPAVTQGGRKISVLANIGDLQDIEAALSHGAEGVGLLRTEFLFHESATLPDEEE
                     QFRVYCSVAQAFGDKPVTIRTLDIGGDKPLPSYPLPAEDNPFLGLRGIRLCLAHPQIF
                     IPQLRALLRAGKEYPTLQIMLPMVSTLEEVNAVKTLIQTQAKLLGLTAENLPALGIMI
                     EVPAAVMIAEKLASEVDFFSIGTNDLTQYIMAADRGNSTVAELVDYRNDAVINAIAMV
                     CQAGRNNEIPVSMCGEMAGDTQQTARLLTMGIDKLSASPSRLPALKAAIRTSH"
     gene            complement(22519..22902)
                     /gene="iraD"
                     /locus_tag="PN977_002652"
     CDS             complement(22519..22902)
                     /gene="iraD"
                     /locus_tag="PN977_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001695400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraD"
                     /protein_id="HDI6042671.1"
                     /translation="MMRQSLQAVLPEISGNKTSSLRKSVCSDLLTLFNSPHSALPSLL
                     VSGMPEWQLHNQSDKHLQSWYCRQLRSALLFHEPRIAALQVNLKEAYCHTLAISLEIM
                     LYHDDEPLTFDLVWQKGSWHRTMPQ"
     gene            complement(22899..22982)
                     /gene="idlP"
                     /locus_tag="PN977_002653"
     CDS             complement(22899..22982)
                     /gene="idlP"
                     /locus_tag="PN977_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iraD leader peptide IdlP"
                     /protein_id="HDI6042672.1"
                     /translation="MENEHQYSGARCSGQAAYVAKRQECAK"
     gene            23574..24404
                     /locus_tag="PN977_002654"
     CDS             23574..24404
                     /locus_tag="PN977_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2686 family protein"
                     /protein_id="HDI6042673.1"
                     /translation="MSMPLSNAANNQFNTNNHFLHHPRVDNKLTREYEYARMDTENIY
                     IMPLVRGNNHNYDGKSVVEIRKLNISKQSWPFNYVTETCREFNGITTTGRMLYRNLKI
                     TSALDEIYGGICKKAHAATELAEGLQLNLFMKSPSDPVEDYTVHEITLGPGCNVPGYA
                     GTTIGYISTLPASQAKRWTNEQPRIDIYIDQIMTVTGVANSSGFAVAALLNANIEMGN
                     DPIIGIEAYPGTAEIHAKMGYKIIPGDEDAPLKRMTLQPSSLPELFELKNGEWNYIGK
                     "
     gene            complement(24545..25318)
                     /gene="uxuR"
                     /locus_tag="PN977_002655"
     CDS             complement(24545..25318)
                     /gene="uxuR"
                     /locus_tag="PN977_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Uxu operon transcriptional regulator"
                     /protein_id="HDI6042674.1"
                     /translation="MKSVTSAQRPYQEVGAMIRDLIIKTPYNPGERLPPEREIAEMLD
                     VTRTVVREALIMLEIKGLVEVRRGAGIYVLDSSDSQNTDGPDANVCNDAGPFELLQAR
                     QLLESNIAEFAALQATREDIVKMRQALQLEERELASSAPGSSESGDMQFHLAIAEATH
                     NSMLVELFRQSWQWRENNPMWIQLHSHLDDSLYRKEWLGDHKQILAALIKKDARAAKL
                     AMWQHLENVKQRLLEFSNVDDIYFDGYLFDSWPLDKVDA"
     gene            complement(25641..>27048)
                     /locus_tag="PN977_002656"
     CDS             complement(25641..>27048)
                     /locus_tag="PN977_002656"
                     /EC_number="1.1.1.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fructuronate reductase"
                     /protein_id="HDI6042675.1"
                     /translation="SRLESRIVHLGCGAFHRAHQALYTHHLLESTDSDWGICEVNLMP
                     GNDRVLIENLKKQQLLYTVAEKGAESTELKIIGSMKEALHPEIDGCEGILNAMARPQT
                     AIVSLTVTEKGYCADAASGQLDLNNPLIKHDLENPTAPKSAIGYIVEALRLRREKGLK
                     AFTVMSCDNVRENGHVAKVAVLGLAQARDPQLAAWIEENVTFPCTMVDRIVPAATPET
                     LQEIADQLGVYDPCAIACEPFRQWVIEDNFVNGRPDWDKVGAQFVADVVPFEMMKLRM
                     LNGSHSFLAYLGYLGGYETIADTMTNPDYRKAAFALMMQEQAPTLSMPEGTDLNAYAT
                     LLIERFSNPSLRHRTWQIAMDGSQKLPQRLLDPVRLHLQNGGSWRHLALGVAGWMRYT
                     QGVDEQGNAIDVVDPMLVEFQKINAQYQGADRVKALLGLSGIFADDLPQNADFVGAVT
                     AAYQQLCERGARECVAAL"
BASE COUNT         7392 a         6636 c         6059 g         6961 t
ORIGIN      
        1 gattaacgca ttgctgtcga tctgggttat gcagcttatt gtttaacaag gagttaccat
       61 gattgattat accgcagccg gttttaccct gctgcaggga gcgcatttgt atgcgccgga
      121 agatcgggga gtttgcgatg tcctcgtcgc taacggcaaa attatcgccg ttgccagtaa
      181 tatcccttct gacattgtac cggactgcac ggttgtcgat ctcagcgggc agatcctctg
      241 cccaggtttt attgatcaac acgttcattt gattggcggt ggcggtgaag caggtcccac
      301 gacgcgcacg ccggaagtgg cgttaagtcg cctgacggaa gcgggcgtca cgtcagtggt
      361 tggtctgctg ggcaccgact ctatctctcg ccacccggaa tccctgctcg ccaagacccg
      421 tgcgctcaat gaagaaggca tcagcgcctg gatgctgacc ggcgcttatc atgtcccttc
      481 ccgcactatt acgggttccg tggaaaaaga cgtggcgatt atcgatcgcg tgattggtgt
      541 gaaatgcgcc atctctgatc accgttctgc cgcaccggac gtttatcacc tggctaatat
      601 ggcggcggaa tcccgcgttg gcggtttgct tggcggtaaa cctggcgtca ccgtgttcca
      661 catgggcgac agtaaaaagg cgttacagcc tgtctatgac ctgctggaaa actgcgatgt
      721 gccgatcagc aagctgctgc cgacccacgt taaccgcaac gttccgttgt ttgagcaggc
      781 gctggagttt gcgcacaaag gcggcactat cgacatcacc agcagcattg acgaaccggt
      841 cgcccctgcc gaaggtattg cccgcgccgt tcaggcgggt attccgctgg cacgcgtcac
      901 cctcagctcc gacggcaacg gtagccagcc gttcttcgat gacgaaggga atttaaccca
      961 tatcggtgtt gccggttttg aaacgttgct ggaaaccgtg caggtgctgg tcaaagacta
     1021 tgatttcagt atcagcgatg ccctgcgccc gctcaccagt agcgtagccg gtttccttaa
     1081 cctgaccggg aaaggcgaaa ttctgccagg caatgatgca gacttactgg tcatgacgcc
     1141 agaattgcgc attgagcagg tatacgctcg cggcaaactg atggtcaaag acggcaaagc
     1201 ctgcgtgaaa ggaacgtttg aaacggctta aacggttgtc agccgcccga atggacgtag
     1261 aatgcccgca agggcggctg acagagtaaa acgtaatgga tggctgtggt gcggttttgc
     1321 ataatattga aacaaaatgg ctttatgatt ttctgaccct ggaaaaatgc cgcaactttt
     1381 cccaggcggc agtcagtcgc aacgtctcgc aaccggcatt cagccgccgc atccgtgcac
     1441 tggaacaggc gattggcgtt gaattgttta accgccaggt gacgccgctg caactctcgg
     1501 aacaagggaa aatctttcat tcgcagatcc gccatctgtt gcaacagtta gaaagcaacc
     1561 tggcagagct gcgtggcggc agcgattacg cgcaacgtaa aatcaagata gccgctgcac
     1621 actctctttc cctcggtctg ttaccgtcca ttatcagcca gatgccgccg ctctttacct
     1681 gggcgattga agccattgat gtcgatgaag cggtcgataa actgcgtgaa gggcaaagtg
     1741 actgtatttt ttcctttcac gacgaagatt tgctggaagc accatttgac cacattcgct
     1801 tatttgaatc tcaattgttc cccgtctgcg ccagtgacga acacggagaa gcacttttta
     1861 acctcgcgca gccacacttt ccgttactga attacagccg caattcctac atggggcgat
     1921 tgattaatcg caccctgacg cgccacagtg agttaagttt cagcaccttt tttgtctctt
     1981 cgatgagcga gcttttaaag caggttgccc tcgacggctg tgggattgcc tggctaccgg
     2041 agtacgccat acaacaagaa attcgcagcg ggcagctcgt tgtgcttaat cgggacgaac
     2101 tggtgatccc gattcaggct tacgcataca ggatgaacac ccgtatgaat cccgttgccg
     2161 aacgcttctg gcgtgaactg cgcgagctgg agattgtgct tagctgacat tattctgcag
     2221 aacatggaat tttgacatta acgccggatg gagattaatc catccggcaa acgcatttat
     2281 tatcttgaat aactcaggcc tttgcttcgt tgaagcgcag taaacggaaa cctgtagaag
     2341 catagatcaa cgcaaatatc atcgagcaga aacagaatac acagtacggc aagtaactaa
     2401 acgtcggcac gcctagtgtt gtcgccatat acaatccggc agctgaccac ggaataattg
     2461 gcgcgctcat ggtgccaaaa tcttccagcg ttcgggctaa gactaacggt gataaattaa
     2521 gatcttcgaa tttcttcatc atcaattcag atataacaat cttattcaaa ccatcagaac
     2581 cggtaagtaa gttaatgatg atcgctaaaa caccgcttgc cagaatagcg ccaaaggcac
     2641 tttttatttt gtttgccgca ctttgtaatg tgacatccat cattcctgtt cccgtcagga
     2701 tgccagcaaa catcatggcg cagataataa atgacaggaa tccgccctga ttattcaggc
     2761 cgccttgttc aagcagttct ttcacagaag gtaacagcgt cgttgaatca gtgatcatcg
     2821 tcagacggaa accactcatt gtggccttaa taccttcggc cagcgggaaa ccttgataca
     2881 gccatccaac caatacggcc acgaacgcac tgcttaataa tgccggtact gtaggcaaac
     2941 gcaaaaaggt aaagcacaga acaataagca taggcagtag cggtaacaga ccaaacttat
     3001 ataactcttt gagctgtacc agcatctgca tgctttctgg tgaaatagag cccgtagaag
     3061 tatgaatcat tcccagccag gtaaaaaaca gcaggctaat aatcgttgat gggatggtcg
     3121 tccagagtag atatttaatc agatcataaa gtttagtttt ggttaccgct gaagtcaggt
     3181 tagtggtatc acttaacggt gaaagtttgt cgccaaaata agccccggta acaatggctg
     3241 ccgccagaat tggaagatcg gcatgtaagc cggaaccgat gcccattaat gcaacaccaa
     3301 tagaagcgac agatccccat gatgccccgg aaacatagga caatactgcg ttcagaaaaa
     3361 acgctatagc ataaagccat gcgggcgcaa ccagtaacat accgtaataa accagcatcg
     3421 gcagtgtacc tgcatagctc catgtcgcga ccataaagcc aacgaaaata acgataagta
     3481 ccggtgccat caccgccgaa atcttatcga taataaaccc ctggatttca tcccaggtat
     3541 atttcagctt agccattgca ataatgcagg aaaacgccgc tgaaattaat aaacagacgc
     3601 tggcaggcca gcccataaca ccgatgccaa caaccaacac gatcatcata aaaatgatcg
     3661 gtgtaagcgc gacataaaaa ctgggttttt ctctcataac tttattccct gttaaaagac
     3721 ttttacgcca ttttgctgta agcgttcctg taccgtctga atatctacct gtgaaacggt
     3781 agtgttattt ttaatggcaa tagcagcggc agtccctgca ccttctccag tgacaataca
     3841 acaccccata ttacggattg tcgcataggc gaaattatct gcatcgattg cccgtccgca
     3901 aaccaacagg ttttcaactt gctgcggaat cattacgcca taagggattt gaaaatagtt
     3961 cgcttcaaga ggtaatttaa gatgaccatt tccatctata aactcaggga atacccctat
     4021 tgaatccgca tgacgtccct cattacaaat atcattttcc gtaagctgga ctcgcccaat
     4081 aatatgtctt gattctcttg ttcccaccgt cataccgaaa ttttttaatt gtgcctgttc
     4141 aaatcctggc tggaattgac gcaacgcttc aatcgcctgc atggcttgct tgcggccttc
     4201 tatttctgca cgcgtcaggt caaaaacatc cgtacaatcg acggcaggga tgctgacaac
     4261 gttcaagtaa ttcgcatcac catactcggt gaccgtactc caggaaccgc ctaacgtcac
     4321 atcttttgga ataattcctg ccgactttcc tttcgcaaag actttaccca gataagggct
     4381 aaacatatcg cgacaagatt catgaacatc ataagaccag tttttatttt cttcatccgc
     4441 cccccagtct ccatatttag gttccgtgct cttaatattt tgcatgaacg cgtttttatt
     4501 tatatttgcg caactaaaaa cggttgtcat acacattagc tcttcgcgtt cacgcttaat
     4561 aaatggtgct ccggcaaacc atgcaatatc ggcatcgcca gtacagtcaa taatgacatt
     4621 cgccaaaata gcctgacgac cagatttact ctctgttatt acgccgagta aattattgcc
     4681 ctgcttaata acatcaacgg cggtaatgtg tagtacgcgg cgcacacctg cctgttcaag
     4741 cattgcatcc gcaaccagtt tgaaacgctc ggcgttaata gcctgactat tcgattgcgg
     4801 ctcagggctg gacgccccca ttgattttgc cgtttcttct atttcgcgtg ccagtccacc
     4861 tgactctacc gtattttcat gacgccacca ggcaattgac tcgacgccag ccgttgtcat
     4921 cattccgcca aaacaaccga aacgttcaat cagcatagta gctgcgcctt cacgagcggc
     4981 actcattgct gcggcaatac ccgatggacc accaccaaca actaacactt ccgtggttgc
     5041 cagtacaggt atattacgag cgggttccag ataaaattcc atactttatc tccgtattca
     5101 ataattgagt aaaacaatcc cccgcgctta tataagcgcg ttgatatttt taattattaa
     5161 caagcaacat catgctaata cagacataca aggagatcat ctctctttgc ctgtttttta
     5221 ttatttcagg agtgtaaaca cattttccgt ctccctggct aataaccaca ttggcattta
     5281 tggagcacat cacaatattt caataccatt aaagcactgc atcaaaatga aacactgcga
     5341 tattaaaatt ctttcaattt gaaaagcatc aatctgcatg ctcaacattt ggatagagat
     5401 tatagatttt aagcttgcgc cataatgttg tacgaccaat ttttaatact tcagctgctt
     5461 tagttagatt ccattcgcag acacgacatg tatgctcaat agcttgcatc tccaattctg
     5521 ccagtgaagt aagatgaggg atatcatcgt cgccactact gttattttgt cggatatcca
     5581 gaggaatatt ctctaatccg aggacaagcc gattgcagtt aataaatacc ttctccattc
     5641 gatttttgaa ctcggagatg tttccaggcc aacggtactc aagcagaact gaatttgcgt
     5701 caggcgtgat tgtcattttt tttcgagtgc gctcctgata cttatcaata atatcgtcaa
     5761 tcatttgctt gagatcttca cgacgtttgc gcaatggcgg aatttcaatt tcattcatgg
     5821 agatctcata atatagctgt cggctaaaag ccccttgttg cacgtactca cgtaaagaac
     5881 tactggtaca tgtaatcagg cgaaagcgta cggggatcaa acgatgactg tctgagcgag
     5941 taacaagccc cgttttcaat actttcaata aaacactctg aacttcccct gatagatatt
     6001 cgactttatc cagatataag gtgccattat gagccagttc aaatttcgat ggcgaaggac
     6061 caacatcaga gccaagaagt tcgtgtaaga tattttctgg tgatagcatc tgacaatcta
     6121 cagaaataaa tggcccatca cgttgctcgc tctcattatg tattgccatc gccaaactca
     6181 attttcccac accttcttcg ccattgatca aaatcggaga gatggattta attgcccggc
     6241 gagcgataag taatacctgc ttcataccac ctgaagccgc atgtaaactg tcaaaggtaa
     6301 aatttgcatt agtgcttatt tgctgttggg ctattttcct gattttgtct aatggatgaa
     6361 gaaaaagcaa aaacgaacca tcgcttaacg ggcgaagggt aaccataagt tcaataaatt
     6421 cgccacggca ttcgaggaca atttgctttt gcgatagttt gttccgttgt gtaattgcct
     6481 cattcaaaat cggcggaaat acgaatcctt ttcggatgtt ctgaccaatt aaactttctt
     6541 tatcaatatt cagtagtgtt tgtgcttgag aattaaccat cgtaattaaa gaatgactat
     6601 tccaacaaat aaaaccgtca tccatgcatt caatcacagc attatgctgg caaagtactt
     6661 tgtttattaa cttttgttca ttctcaatat agatataatt agcgatttct gttgcacatg
     6721 aatatgatgt ttttaattta acaagctcgg cggtagtttc aattatacat acaatgtatc
     6781 catggatatt accataataa tcaatgaccg gagcagcgct catcgcataa caatgtagct
     6841 gttcactaaa atgttctgct ccttccagtt gtgtatataa atttgtttcc cgacaaagac
     6901 tatatacatt ggttccaatg ccatcttcgg cccatgacat accagattca attcctaact
     6961 ctcgcaattg actggaataa tcgcctgctg cattcaacga tagaacccag ccgtgtgggt
     7021 caatgatcaa aaatgccaac cggtcgtcac cagccagggt gaaatggtct tcaacaactc
     7081 tttgtgaaat tctaataaac tcttcgttac gagaaaaaat cgtctgcaat gtttgcccgg
     7141 aagctttgac aggcgtgttc cagtggcgag ggtcacaatg cttgagacat ttttgccatg
     7201 acctcagcat cacatgatta gtcgaatttg tcgcgaccca accatgctta acaaacattt
     7261 tccatttatt ttttagatca ttatgatacg actctttatt cattataata atatccattc
     7321 aagtcatttt cagaacttgt atcagaatgc tatgccagta agcttgatca aacgcaataa
     7381 tatctagtct ggatcattac aaaaacagac cttatcaaca atgtaaccat acatacatta
     7441 gaaagaataa ccgttaaagc aataaaaaac gaataataca ttagataaat tttaaaaatg
     7501 ccaacaacat ctgaaatctt tgcgtttgat atttatcatt attcaaactg aaacaacaca
     7561 ggcatacgag ttgtttcact gtgaaacagt gcagcgaaat ttgttctgcc cttttatttc
     7621 ctcaaagata tttcgtcgtt aaattccaaa attgactgac tattcaactc agcgtaaata
     7681 tcaggagaaa taatgtatgg cgaggatgcg tgcaatcgaa gctgcagtag aaatactgaa
     7741 aaaagaaggc attagcgttg cttttggtgt acccggcgca gcaatcaacc cactctatgc
     7801 cgcgatgaaa aaatcaggcg gtattgatca tattctggcc cgtcatgttg agggcgcctc
     7861 gcatatggct gaaggttaca cccgcagtca gaatggcaat attggcgtgt gtattggcac
     7921 atcaggccct gcgggcactg atatgattac ggggctttat tccgcctccg cggattccat
     7981 tcctattctc tgcattactg gtcaagctcc cgtaggaaaa cttcataaag aagatttcca
     8041 ggccgtcgat attgaggcta tcgccacgcc agtaaccaaa atggctcgca caattctcga
     8101 agcgggtcag ttgccgggga tattccagaa agccttctgg gaaatgcgtt caggccgccc
     8161 tggcccggtt cttttagatc tcccttttga tgttcagatg accgaaattg agtttgatat
     8221 cgatctctat cagccactaa ccccctggca acctcaggca acacgggccc aggccgtacg
     8281 cgcattagaa atgctaaatg gcgccgaaaa gcccgtcatc atcgctggcg gtggcattat
     8341 taacgccgaa gcgagtgaat tgttgcgtga gtttgtcgag ttaactggcg ttcccgtcat
     8401 tcaaacctta cgcggttggg gcgcattatc agatgatcat cccctaatga ttggtcgcat
     8461 gggatgtcag gcgggtcatc gctatggtaa cgccagttat ctggcctccg attttgtttt
     8521 tggtatcggt aaccgttggg caaaccgtca taccggagcc attgagacct acaccgaagg
     8581 ccgcaaattc attcatgttg atatcgaacc tgcacaaatt ggccggatat ttgcgccgga
     8641 tctgggcatt gtttctgatg ctgaaagcgc cttaacgcta tttattcagg tcgcccgtga
     8701 tatgaagtca cgtggagaac tgaaagaccg cagtcgctgg attgccgaat gtgccgagcg
     8761 taaacgcaca atgcttcgtc gttcagactt tgactgcaat ccaattaaac cgcagcgcgt
     8821 gtatcacgaa atgaataagg ttttcggacc ggagacacgc tatatttcga caattggtct
     8881 ggcgcaaatc gccgcaaacc aatttttaca tgtttaccgc ccacgccact ggattaatgc
     8941 ctgtcaggct gggccgctag gctggacaat gcccgcagca ctgggcgcgg taaaagctga
     9001 tccatctgtt cccgttgtgg caatatccgg tgattacgat tttcaattct tgattgaaga
     9061 attagccgtc ggcgcacaat ttaatcttcc gtatatccat attttactta acaacgctta
     9121 tcttggtttg attcgtcaat cacaacgtgc atttgatatt gattattgcg ttcaactgtc
     9181 atttgaaaat attaatgctc cagaaattaa tggttatggc gttgatcata aagctgtggt
     9241 tgaaggatta ggatgtaaag cgattcgcgt attcgcatca caggatattg cgcctgcact
     9301 gcaagaagcc cagcgtttgc gtgacgaatt tcacgtacct gttgttgtgg aaattattgc
     9361 tgaacgcgtg actaatatcg ctatgggacc tgacattaat aaagtcacag aatttgaaga
     9421 aattctcgat ttataaggaa acaggattat ggcaaagttt gcagcaaatt tatctatgct
     9481 ctttaccgag tatccattta ttgaacgttt tgcacaggca agtcacgcag gattccacgg
     9541 cgttgagtat cttttcccct atgacttttc tgcagatgag ttggtttcgc aactgcatca
     9601 acataatctg acgcaggttc tgtttaatct tcctgcgggt aactggcaag agggtgaacg
     9661 gggtattgca tgtcatcctg gtcgggcaaa agaatttcag gagagcgttt ccagggcaat
     9721 agagtatgca ttggccctta attgttcaca agttaattgc cttgcaggaa aacatccagg
     9781 tggttattcg catgaacaat gtcatgaaac actggtcgaa aatttgcgct atgcggtaga
     9841 taaattggct tctcatggga taaagttagt tctggaagcc attaatacaa aagatattcc
     9901 cggttttttt gttaacaata ccagacaagc tctcaatatt attcatgatg taaaacatcc
     9961 tgatttgcgc taccaatacg atatttatca tatgcaaatc atggaaggga atattgctac
    10021 aacaatcaaa aacaacctga ataacattgg acatattcaa ttagccgata accctgggcg
    10081 tcatgaacca gggacgggag aaattaatta cccgtggttg ttaaattata tagaccagat
    10141 tggttatcag ggatggattg gttgcgaata tattccatct acgacaacga ctgaaagtct
    10201 acgctggcta aaaaacgaga ctcaatttta atccctgagg tatttatcat gaatattgga
    10261 tttatcggca ccggcataat gggtaagccg atggcttata atttacaaca agccggacat
    10321 actctttatt tttcagcgca ttttgaacct gcaccgcaag agcttattgg tgaacgtggc
    10381 attgtttgct ctacaccgcg tgaagtcgcg caggagtgtg aggtcatcat caccatgctc
    10441 cctgataccc cacatgttga ggatgttctt tttcatccca actatggcgt aatacacggt
    10501 ttaagccacg ggaaaattgt cattgatatg agttcaattt ccccggttgc caccaaagca
    10561 tttgcacaac gtattatcgc gggaggcgcg gaatatatcg atgcgcccgt ctctggcggt
    10621 gaagttggcg cgaaagccgg gacgctttcc attatggttg gcggatgtga agaggtttat
    10681 ttacagatca aaccgattct ggaattgatg ggtaaaaata tcactctggt cgggaacgtc
    10741 ggtgatggtc aaacgtgcaa agtcgctaac caaattattg ttgcactgaa tattgaagcg
    10801 gtggctgaag ccctgctctt tgccagtaaa tcaggtgccg atcccgcccg cgtacgtgaa
    10861 gctctgatgg ggggttttgc ttcctctcgt gtattggaag ttcatggcga acggatgatc
    10921 aaagggacat tcgcccctgg ttttcgaatt agcctgcatc aaaaagatct gaatctggca
    10981 ctggagaatg ctcgcctgtt gaataccccg cttcctaaca cggctatgac gcaacaatta
    11041 tttagcgcct gcgccgcgct cggtggtaaa gagtgggatc attctgcgtt aattcgggcg
    11101 cttgaaacac aagccaattt cactatccgt aaataatgaa ttaagggaac caccggttcc
    11161 cttcgttcac tcgttgccct gccccattcc cctattttga gagacctgct atgaagatag
    11221 ttatctcccc cgactccttt aaagaatgcc ttcccgcatg gaaagtcgcc gaagcgctgg
    11281 caaccggctg gcgcaaggtc ctgcctggca gccaactggt gtgtttgccc gtggctgacg
    11341 gcggcgaagg cacgctcgaa acactcattc atgcgactga cggtacgttt tacactaaaa
    11401 aagtcaccgg accgcttggc gaatcaatac atgcgcaata cggtatttta ggcaaccaaa
    11461 ccaccgcagt gatagaactg gcacaagctt cagggctgga actggtttct cctgtccagc
    11521 gttctcccct ttatacgacg tcgtttggca ctggcgaact tattctcgcc gccctggaac
    11581 acaatattga taccgttatt ctatgcctgg gtggcagtgc tacaaatgat ggcggtattg
    11641 ggttgatgtc ggcacttggc gcatcgttta cagacgccga agggctatca gtctctgtta
    11701 atgggatggg gctggcggca attcaccaca ttgacttaca gcacctcgat ccacgattga
    11761 aaaatgtgaa atttattgca gcctgtgatg tcaccaaccc actaaccggc gataacggcg
    11821 cgactcgggt ttttgctcaa caaaaagggg ccagtgctga caaccttgag caactggaac
    11881 agggaatgaa aaactatgcc cgttgcatct accgttgttg tggtaaagaa gtcgatacga
    11941 tacccggttc tggggcggct ggcggcgttg gcgcggcctt gatggctttt ctcgatgctc
    12001 gcttacaacc gggtatttcg ctcgtgctgg aagcgattca atatacccaa catttaaaat
    12061 atgcagcatt ggcgattgtc ggtgaaggta aattagaccg tcaaagcctg aatggcaaag
    12121 cacctgtggg ggcggccaaa atcgcccaga tgatgggcgt tccggttatc gcaattgccg
    12181 ggtatatcga tgatcaactt gatttgaatg agttacgcca gtgtggaatc gaagcctgtt
    12241 tttccgtcgt caatggtcct tgtgatttac ccaccgcgct gagtcagggg gaaaataatt
    12301 taattcgtct cggagaaaat ttggcagggt attttcatgc agtcctgagt taattcccgt
    12361 tcggtaaaat gcccgataat gcaaattacc gggcattcct gaacatcatt attcatctct
    12421 cgcggttttc tcttcagccg caagctgtgt caaaaaactc ttcaataaaa taaacactac
    12481 gccgcaaagc ggtagtccgg ccaggataga gaccgtttgt agcggttgca gactttgcgc
    12541 atcaagaaga tagagtgtga ttgcgacgat tccggtcata atcaaccaaa atgccacgtt
    12601 ccagaatgcc ggttcactct cactatctgc ccgtttttgc gtcgcaaaac cgagggcata
    12661 acccacagta cagtgtccgg taatatatga aattaatgct atagccccaa aagcaaccaa
    12721 aaacacctga cttgcgggta gctgctccag taaacgagaa acgacaacgc ctcgcccttg
    12781 ttcgttaagt atgggaacaa aatcgatgac atgagttgtc tgcaacgata agccgaaatt
    12841 agccagtacc atgtaaaaaa ccatgcatcc caatgaacct gccagagttc cacatacaat
    12901 caattgccgg atcgtccttc cttttgaaat acgggcgata aataaaccga atgggatggc
    12961 atacacgacc caccatgaaa aataaaatac ggtccacatc tgtggaaatc cgctacgacc
    13021 aatagcatcg gtacttaaac tcatcgctac aaaattttgc agcatcaggc cgaaaccatc
    13081 aattgattgg ttaagtataa aagctgtcgg tccacaaatc agtattgcca gtagcataaa
    13141 aatatcggca cgaatattcc agttactggc ccatgccaca ccttttttaa gtccaaaaaa
    13201 gagaattaat aatggcacta gcgtccagat aaaaataacc atgacattga taaactgccc
    13261 ggcaggatcc agatggaaca catggcaaaa tacggcagaa atcatcggcg ttcccagcgc
    13321 cagagacgtt accgcgccgc cgagcaaacc aaacatgtag aaaaaattca cacaatgccc
    13381 gcaaaaacca tcagcacgtg cccccagaca cccacggcat aaatcactga cctttaatga
    13441 gttcgttttc ttaacatgca taaaccaacc aatgggaacg gcacaaatca gataccatgc
    13501 ccatgcggac ggcccccaat ggaacatgcc ataagcagag gcccaacgag cagcctgagc
    13561 gcttcctgct gtcgcgccaa aaggcggttg ctgaatgatc caaatccact ctacagaggc
    13621 cagatataac aagctgcccc ccgttccaga agtaaaaata agtccaagcc aggttaacaa
    13681 gctgaagtca ggcttttctc ccgactgacc taacaagata tcgccataac gtgagaaggc
    13741 cagccacaga caacaaagta caaccagaaa agtcatgacc agaaaaatcc cgcccatttc
    13801 ccaggttaaa aaatgatgta ttttatttat ataccggctg gtatcttgtg gatatatcgt
    13861 caaacacaga atgattaccg caataaataa tattgctggg ataaacacat tcagttcaat
    13921 caaagaaaag cgttttttta aatattgcat ttcagtctcc tgaatgtttt cattcaatga
    13981 ggacgaatat ttcgccgttc ctttttcatg tgttgcaatt gtcgggaaag gcgtaaagaa
    14041 aaaaaggcgc aaataagtga aagtaccgca atacaataca gagctaaacc acactgatgc
    14101 tgttgcagca tgaacaggaa tatccccaaa aagaggccgc agagaactgt ggttatccag
    14161 taattggcac ttaagtgctc caggtcatca tccctcatgg tctttctcct gcaaggcgca
    14221 gagaggcttt cagctctctg cgccttgttg ttatgccaga cgtacgccct gctgttgcag
    14281 cgtgttttgc aaatcaggaa tagcaacctg acgcacggtg caatgttgct gtagcgaaac
    14341 cacggcggca gtgcctgcgg cctcaccggt aacggcacaa cacatcatgt tgcgcataga
    14401 agtatgtgca actacgcctg cggaaataca gcgaccggcg accaaaaggt tctccacttt
    14461 ttgtggcacc agacagccat aggggatctg aaagaaacgc ccggtcgttg gcaaaatgag
    14521 ataaccggaa ccatcaataa attccgggaa aatccctaca ctgtccgaac aacgcccctg
    14581 attaaggacg taatcatcag caatacgtat ttccccctca ataagccgtg attcgcgtgt
    14641 acccagcgtg gcgccaaaat tgcgtaaccg tacattttca aagcccggaa cataatggcg
    14701 tagagcgtca attgcccata atgcttgctg ccttccggcg atttctgctt tggttaaatc
    14761 gaagacatcg gtacagtcaa aaccaaaggc gtacaccata ttcatctgaa acgcttcgcc
    14821 gctttcagaa aaggttgacc aggttccggc aatcgcctgg gcgtcgcctg gaatcacacc
    14881 atcctgctgc gcccgggtaa aaatatcctc catataaggg ctaaacatct ggtcttcttt
    14941 gcccgtggtt tgaatcgtcc agttttttcc ccaatccgcg taggtcggct taagttcttc
    15001 cgcaacaaaa cggttaaagc gcgccacatc gacacctgcg caactgaaca tcacggtgac
    15061 gcccatcagt tggtcctttc cccgtttcgt ccagggcgcg cctgcacgag cagcaatatc
    15121 agcatccccc gtgcaatcga cgattctttt cgccagaatt gcccctcgtc ctgatttgtt
    15181 ctcgacaata acaccgcata acgtatcccc ttccttgatc acatccacaa cccaggagtg
    15241 atataacggc tcaacgccag cttccgttat catctgatcg gcaacaactt taaatccttc
    15301 agtatctata acttcgctaa ttgactgagg ttctggtcgc gtgaagccca atgctcgcgc
    15361 gcggccttca tattcacgac aaatcccttc acaatcttcc gtccccggat ggcgatacca
    15421 ggcaaaactt tccacaccaa cctgcgtcaa tacgccgcct agtgagccgt aacgctcaac
    15481 aatcatggtt ctggcaccta gccgccccgc cgctatcgcc gccgttaatc ctgccggacc
    15541 gcccccgata accagaacgt ccgtttgtgc aatgactggc gtttgccgtg cactttcatt
    15601 aatcatgtcc accattggtt cccccatcag attcaggatg cgcaatccgc attgttttca
    15661 gtgatttcat tgacttgatt tttactttta cgattcacac gctggaggac cagtgcggcg
    15721 ataaggccga tgaatgcgca aaccaacatg aacaggaaaa catcctgata accggcaata
    15781 cccggtttgc ggtcgaccat attgcccacc atcgtgtagc agtagatttc cgggaaatag
    15841 gtcagtaaag agatcagacc cgcagcagtc cctgtgactt ttgccgggat ccgaatctca
    15901 tcaataattg agaagaacac ggcattcgca gagtaaataa acacgcccaa cgcaaccata
    15961 ttcgccagcg caatagtgac gtagcgcgac tcaccgggca ggaagaaata aacggtagta
    16021 aagacaatca tgccgataaa ggcgtaaatc ataaaacgcg tgcgtgacgc acagcggtcg
    16081 gccagttgtc cgccaattaa tccccccatc gcaaataaca cataggcgcg aagaatactg
    16141 gcaactgcca cttccgattc gcccatatgc aggatctgcg tgagatacgg cgtgaccatg
    16201 ccaaagccga tataaattga ccacatacaa aaggtcacaa cacctgccag ccaaaccgtc
    16261 ggcattttca gcacgaatgc catatctgcc agacggaaat tcgctttatc ttcgctgtgc
    16321 gtttcatcct gacaaacaaa ccaggctaaa acaccggcca gaatagtcac tacggaataa
    16381 aaaataattg tcgcccgtaa accatcaaca ccttcgccaa atttggcaaa taccggcacg
    16441 gaaagaaatc ccagtacaat ggaggttaat cccttaccca gaaaccagta accgtataag
    16501 cgaccttgtt cttcgctggt tcccagggta cgaataattc gcacacacac ggcccaaaac
    16561 gtaaatacgg ttgtaacagc ccaaagtgca tgtaacaaac agatcatagt atacgagggg
    16621 aaggtcgcat aataaaaacc acttatcccg gtggaaatta atgaaaaggt cattaattta
    16681 cgtgcagaaa atctatcggc agcccaaccg ccaggtagat ataataaaaa gttcaccaga
    16741 ccataggctg tcattagaaa accaagttca gcattagtcg ctcctgtagc ctgctgcatc
    16801 gcgtcgtaat aggtttcccg taaataaggc aatttatata ttgcacttcc ggcaatacca
    16861 cataacgcca gcgtgatcca acgtttccag ttactggtca tatttattcc cgaatgaagt
    16921 atttaaaatg acaccaacgg cctgctttgc acaggccgta ttattttttt aaagtcccaa
    16981 atgatgtgca gttaaaattg cgcaaagaac gtcctgcgct gttactacga atggcatatt
    17041 atggatagtt tccccttcag cagtcgctgc ttcagccact gcccgtaatt tatctttatt
    17101 aatttccttc acgcctaaca tatgaagatt tgtcggcaga ccaacgctat ggcagaaatt
    17161 gagtacggtt tcaatttcct cctgtggtgc gttttccagt acgagctgta ccaggacgcc
    17221 aaatgccact ttttcaccgt ggtagcaatg atggcattcc tccagttgcg tcatgccgtt
    17281 atgaatggca tgagctgccg ccaggccgct actttcaaat ccaatgccgc taagataggt
    17341 attagcttcg ataatattct ctaccgcttt ggtgctaaca ccttgttcaa ccgcgacttt
    17401 tgccttatag ccatcttcca gtagcgtggt ataacagagt cgggcaatag ccagtgccgc
    17461 cagtgtcgac gccccacctg ccattgtggc ttttcctgac gcctgattgg ctctcgcctc
    17521 aaaccaggtt gacaatgcat ctcccatacc ggccaccagc agacgcactg gcgcggctga
    17581 aatgacgcca gtatccataa tcaccatatc cgggtttttc gggaaaaaca gatattcgct
    17641 aaactggcct tccggggtgt aaattactgc cagagaactg gttggcgcat ctgtggaagc
    17701 aattgtcggc accaccacta ccggaatttt ttggtaatac gctacggctt ttgccgtatc
    17761 caacgtttta ccgccgccaa taccaataac gacattatat ttacgctgat taaatttctc
    17821 acgaattcga gaaatttcat tttgcgagca ttcaccgcaa aaaacatcaa gatccgcaat
    17881 aatgttttct cttgcaaagc tctggcttac tctgtcacca accagaccgg tcacaaattc
    17941 atctgcaata attaatggtt ctgttcccaa actcgccgca tatgtagcaa tattatccag
    18001 agaaccattt ccctggatat atttagccgg acttataata atcttatcca tatcattcct
    18061 cgcgtaactt gttttgcttc actgttaacc tactcgttgc atccagtggc ggcaaacatt
    18121 tcattcgcga aaaattatgt tttatgatcg cgctgtaaaa aacatcatct gcagatgata
    18181 ataaaaaaca cttttcatgc cttaatcaca atatatcaat atccatactg gtcccttttc
    18241 attttgaaac aacaccatga attaactgtt tcattatgaa tcttattatc gtgcgatttg
    18301 ttttattgcg gttggagaat taatctcgca aacatattta gcgcgattta cgtaatttat
    18361 cagaacagga atgactatga agaaattaat taaccagatt gattctgttg tcacagaaca
    18421 aatggaagga ctcatcgcca cctggccaca cctgcaggca aattacgcgc cccgatatgt
    18481 gtggtgcaaa caaaccgata atgcagtggc gctgatttca ggcggcggct caggccatga
    18541 accactgcat gcaggttttg tcggtatggg aatgttaacc ggtgcatgtc ctggtgaaat
    18601 cttcacatcg cccacgccag atcaaatgat cgaatgtgcc aaagcggttg ataatggttc
    18661 tggcgttctg tttttcatca aaaattacac aggcgatatt ctgaatttcg aaacggcagt
    18721 cgagatgtta cacgaagaag gcatcgccgt tgggaccgtc attattgatg atgatgtagc
    18781 ggtaaaagat agcctgtata cagccggacg tcgcggtgtt gcgggtacag tttttgttga
    18841 aaagattgtc ggtgcggctg cacttcaggg atataacctg gggcagtgcg agcaacttgg
    18901 caaagatgtt aacaatgcca cccgttcttt tggcatcgca ctgtcagcct gtacggttcc
    18961 cgccgcggga aaaccctcat ttgaactggc tgataatgaa atcgaatttg gtgtcgggat
    19021 ccacggtgaa cccggcattg aacgccgcac tttgcaagat ctgaacactt taatcgacag
    19081 cgtgatagcg caattactgg ataatacccc ctggcgacgc acattacgcc attgggatcg
    19141 ccatgcgggg ggatggattg acgcatcatc aatgaatgag tcattcgatc aaaatgccga
    19201 atatattgtg ttaattaacg gacttggcag cacgcctgaa tcagagttat atggtgtggc
    19261 cagggtattt atgtgcgccg cacagcgcca gggaattaaa attagccgcc agttggtcgg
    19321 taattattgt acatcattag atatggccgg attttccatc agcctgctga aatgtacccc
    19381 agagtttttg caactttggg atgccccggt caatacgcca gcgcttcgtt ggggatgtta
    19441 ataaggacat tatatgaaaa ttcaaaacaa acatgttatt gcctggctgg agagttgtgc
    19501 tgcacatctc actgaacaac aagattttct aaccgcactt gacagagata ttggtgatgc
    19561 cgaccacggc ctcaatatga atcgtggttt cagtgcagtg aaagcgacgt tgcccgacat
    19621 tgaacgtcag catattggca atattcttaa aaatactggc atgaaactac tttccagcgt
    19681 tggcggtgcc agtggtcctc tttacggcac attatttatt cgcgcttctg cggcagtggg
    19741 agccagaaca gaattaactc tggaagagtg gctggcgtgt ctggaagaag ggattgcagg
    19801 cgttatcgca cgaggaaaag cagaacaagg ggataaaaca ctgtgtgatg tctgggttcc
    19861 agttctccac gaagccaaaa agaacctgca agccggaatg tcgccttccc ttctgctgaa
    19921 tacgatggtg caagatgcgg caacagcagt cgataacacc atcaatatgc aggcgaaaaa
    19981 aggacgtgcc agttatctcg gcgctcgcag tgtgggccat caggatccag gcgcgacctc
    20041 ctcctggctg atgattaagg ctatgcagga aggattcgcg ggataaatta tggttgccat
    20101 tgttattgtt tctcatagtc tacgtctcgc ccagggggtt gaggaattag cactacagat
    20161 gtctggaggg gacgttcctc tggccattgc tgcaggtatt gatgatccgc aaaatccgat
    20221 tggcactgat gctattgcca ttatgtctgc gattgaatcg gtctggtcac cggatggcgt
    20281 tctggtactc atggatatgg gcagtgcgct gcttagtacc gagatggcgc tggagctgct
    20341 ttctgaagag caaagaagtg caatttatct gctcgccgcc cctgtagtgg aaggtgccat
    20401 gtcagcggta acgtctgccg cagcggggct ttcggtgacg gagattatcg ctgaagtgga
    20461 tcttgcgctg tgtgctaaac agcagcaact cacgccatcc tcaactgcag gcgaggttat
    20521 tccagcaata tcaccaatca atcattccgg tgaatggcaa actttttgct ggacgatacg
    20581 taatccccac ggcattcatg ctcgtccggc agccagcatc ctgaaagtat ccgcacaata
    20641 ttcggcaaac attatcgtca tcaaaggcga taaacgcgcc agcacccgca gtctcaacga
    20701 actggcaatg ctgggcgtcc gctgtggcga tgaaatcatc ttccaggccg aaggcaccga
    20761 tgccagtgac gctctcaagg caattgaaac gttggcgaaa aatcattttg gtgaagctca
    20821 cctgctcgct tcttcagaga ttccgctaac ggaaacgccg gtggtcagcg cccctgtcga
    20881 tggcgcaata tcaggcttgt cagtacaaaa tggtatcgct attggtcctg ttaaatggtt
    20941 tacctgcgaa cgaccagaaa ttacgcagcg caccgtcgat tctccgcaag aagagctgtc
    21001 gcgaatcgag tctgctatcg acattgttgt ttgtgaactt gccgataaag cggcaggccc
    21061 tgaaggcgat atttttgccg cccataaaat gatgcttgaa gatccaaaga ttaaccggca
    21121 attacagcaa cgtctggcaa agggcaaaca ggcggaattc gcatggttag aagtcatgca
    21181 ggcattagca gagcaatatt gtcaggcgga aacactgtat ttacgtgaac gcgaggcaga
    21241 tatccgtgac cttacacgtc aggtactgaa ccagctttgc gggggaagtg aacaacactt
    21301 tattactacc gcgccctgca ttttgttagc caacgatctg ttgccatcac agataaccag
    21361 cctgaataaa gcacacattc ttggcatttg tcttcataat ggcggaacaa cttcacatac
    21421 agcgatcctt gccagagcaa tgggtattcc tgccattgtg aaggcagcta ttaccccgca
    21481 aaacgtccgc gataacgaca cggtcattct tgatggtgaa acaggccgtt tgtggctaca
    21541 gccggatgag gtcacccgcc tggatttact gcaacgcgcc gaagcatggc ggcaacaaag
    21601 ggatcgtcag ttagctgatg ccatgcagcc cgctgtcacg caaggtgggc gaaaaatcag
    21661 cgtgctggcg aatattggtg acctgcaaga tattgaggct gcgctatctc acggggctga
    21721 gggcgtgggt ttactgcgca cagagttttt attccatgag tctgcaacat tacccgatga
    21781 agaagagcag tttcgcgtgt attgttctgt tgctcaggct tttggcgata aaccagtcac
    21841 catccgcact ctggatattg gcggtgacaa gccattacct tcgtaccctt tacccgcaga
    21901 ggacaacccg ttcctgggac ttcgcggtat ccggttgtgc ctggcgcatc cgcaaatatt
    21961 tattccgcaa ttacgcgcac ttctccgggc cgggaaagaa tacccaacgc tgcaaattat
    22021 gctgccgatg gtttcaacgc tcgaggaggt caacgccgtc aaaacgctga tacaaacgca
    22081 ggcaaaactg ctgggactta ctgcagaaaa tcttccggcg ctgggaatta tgattgaggt
    22141 tcctgctgca gtcatgattg cagaaaaatt agccagtgaa gtcgatttct tttccatcgg
    22201 caccaacgat ctgacgcaat atattatggc ggcagacaga ggaaatagca ccgtcgcgga
    22261 actggtcgat tatcgtaatg acgcggtgat taacgctatt gctatggtgt gtcaggccgg
    22321 acgtaataat gaaattccgg tcagtatgtg cggcgaaatg gctggagata cccagcaaac
    22381 agcacgactg ctcaccatgg ggattgataa gctcagtgcc agtccgtccc ggcttccggc
    22441 cttgaaagca gcaatcagaa cctcccacta attgtgacct aacgcgatac gtcttgcgcg
    22501 tatcgcgttt cccgtcattt actgcggcat cgtgcgatgc caactcccct tctgccagac
    22561 cagatcaaac gttagcggtt catcgtcgtg gtacagcatt atctcaaggc tgatcgccag
    22621 cgtgtggcag tacgcttctt tcaaattgac ctgcaacgcc gcaatccgtg gctcatgaaa
    22681 taacagtgca gaacgcagct gacggcaata ccagctctga agatgtttgt cgctctggtt
    22741 atgcagttgc cactcgggca tgcctgaaac aagtaatgac ggtaatgccg aatgaggaga
    22801 attaaaaagt gtgaggagat cgctgcatac cgatttacgc agcgatgacg ttttattgcc
    22861 tgaaatttca ggtaaaacag cctgaagtga ttggcgcatc attttgcgca ctcctgacgt
    22921 ttagcaacat atgcggcttg ccctgaacac cgggcaccac tgtattgatg ctcattttcc
    22981 atagccattc ccttttgttc attacatcca tgtcagtctg agacaggcct gaatcctgtc
    23041 agcaaagtat aaaaccttac ttgcctatga atatctactg acttttcagg aaaaaagtca
    23101 tgatttgata aaaatcatca aactcaaaaa aaaactgcta gtatattctg aatatttcga
    23161 atagttatgt agatgaaaat acttgtcacc gctgctactt aattccggtg ggaaattatt
    23221 tatttccgca taatcagcca tgtactattt gaaacaatta gtttgtaaat aatataaatt
    23281 gataacatat caaatacatc aaaaatacca ttcataggca ttattaatat agacataaat
    23341 atagcaacat caaattccaa ttagtaataa gatatgcttt ttaattgcat gaaaataatt
    23401 tcaaatgagc gttttttgat actttagagt gtttcctacc ctttaatatc accgtgtaga
    23461 gttgatatca ctacaacaga aacatgcatg tcattttttt acttcttttt tactacagaa
    23521 aaatttatct atttttctaa acatcagtga aatactcata aaaggtaact attatgtcta
    23581 tgccattaag caatgccgct aataaccaat tcaatacaaa taatcacttc cttcatcacc
    23641 caagagtcga caataagtta acacgtgaat atgaatatgc acgcatggat acggaaaata
    23701 tctatattat gcctcttgtt cgtggcaata accataatta cgatggaaaa agtgttgtcg
    23761 aaattagaaa acttaatatt agcaaacagt cctggccatt taattatgtg acagaaacat
    23821 gcagagaatt caatggtatt acaacaacag gacgtatgct ctatcgaaac ttgaaaatca
    23881 cctcagcttt agacgaaata tatggtggta tatgtaaaaa ggcgcatgct gccacagagt
    23941 tagcggaagg attacaacta aatttattta tgaagagccc ctccgatcct gtagaggact
    24001 atactgtgca tgaaataaca ttaggccctg gctgcaatgt tcctggttat gccggaacca
    24061 cgataggata tatttcaacc cttccagctt ctcaggcaaa aagatggacc aatgaacagc
    24121 caaggattga tatctatatt gatcagataa tgacggtaac aggggtagca aattcttccg
    24181 gattcgccgt tgccgcatta cttaatgcta atattgagat gggaaatgac ccaataattg
    24241 gtatagaagc ctatcccggt accgcggaga tccatgcaaa aatgggatat aaaattatcc
    24301 ccggtgatga agatgcgcca ctcaaacgca tgactctgca accgtcatct cttccagaac
    24361 tgtttgaatt aaaaaatggt gaatggaatt atattggtaa atgactactg agaagaatga
    24421 gtactaacat tcaggttata cagatattta aacaataccc tgcaatttac aaagtaatac
    24481 tcattgcgat ttcttaattt aagaaagcgc aaggaacgtt tacccttgcg cttatcataa
    24541 taagtcaggc gtcgacttta tccagcggcc atgaatcaaa cagatagccg tcaaaataaa
    24601 tatcgtcaac gttcgaaaat tccagcagac gttgtttaac gttttccaga tgctgccaca
    24661 ttgccagctt cgccgctcgg gcatcttttt tgattaacgc ggcgaggatc tgtttgtgat
    24721 cgcccaacca ctctttgcga tacaggctgt catccagatg gctgtgcaac tggatccaca
    24781 tcggattgtt ttcccgccac tgccaggact gacggaatag ctccaccagc atgctgttat
    24841 gcgtcgcttc ggcaatggcg agatggaact gcatgtcacc actttcgctg ctgcccggcg
    24901 cgctggaagc aagctcacgc tcttccagtt gcaatgcctg acgcattttg acgatatctt
    24961 cgcgggtagc ctgcaaagcg gcaaattccg cgatgttact ttccagtaac tgacgcgcct
    25021 gtaacagctc aaaaggaccc gcatcattgc aaacgttggc atccggaccg tctgtgttct
    25081 ggctgtctga gctatcaaga acataaatac cggcaccccg gcgcacttcc accagccctt
    25141 tgatctccag catgatcagc gcttcacgta ccaccgtccg cgtgacatca agcatttctg
    25201 caatttcacg ctccggcggc agccgttcgc caggattgta cggcgtcttt atgatcagat
    25261 cgcggatcat cgccccgact tcctggtaag gtctttgcgc agaggtgaca gatttcataa
    25321 gtaatatcca tttgatgaga ctgataacaa atcctgtaaa gggcacgttc agtcccctcg
    25381 cccctttggg gagagggtta ggatgagggg aaatgactgt agccattacc ctgccctatt
    25441 gccttcctgg actggggaga aaaacagtga ctgaatcgct cataaacagg tttgtcagca
    25501 atctggttag atgcggttat ttattttcaa actataccat tagcactgga agttggcacg
    25561 tacaggtggg caaaaaaacg aaaactggtc aaccaaatat cagtttgatt gaccagtaag
    25621 tctgtagggt aatcagttag ttacagcgca gccacacact cgcgcgcacc gcgttcgcac
    25681 agctgctgat atgccgccgt caccgcgcca acaaagtcgg cattctgcgg cagatcatcg
    25741 gcaaaaatac cgctcaggcc cagcagcgct ttcacgcggt ctgcgccctg atactgcgcg
    25801 ttgatcttct ggaactccac cagcatcggg tcgaccacgt caatggcatt accctgctca
    25861 tccacgccct gggtgtaacg catccagcca gccacgccca gcgccaggtg acgccagctg
    25921 ccgccgtttt gcaggtgcag acgcaccgga tccagcagac gctgcggtaa cttctggctg
    25981 ccgtccattg caatctgcca ggtacggtga cgcagagacg ggttgctgaa acgctcgatc
    26041 agcagcgtcg cataggcgtt caggtccgta ccttccggca tcgacagcgt tggcgcttgt
    26101 tcctgcatca tcagggcaaa ggccgctttg cgataatccg ggttagtcat ggtgtcggca
    26161 atggtttcat agccgccgag gtaaccgagg tacgccagga aagagtggct gccgttcagc
    26221 atacgcagtt tcatcatttc gaacggcaca acgtctgcaa cgaactgtgc gcccacttta
    26281 tcccagtccg gacgaccatt aacgaagtta tcttcaatca cccactgacg gaacggttcg
    26341 caggcaatgg cgcacgggtc gtaaacaccc agctggtcag caatttcctg taaggtttct
    26401 ggcgtcgccg ccggaacgat gcggtcaacc atggtgcacg ggaaggtgac gttctcttca
    26461 atccatgccg ccagctgtgg atcacgcgcc tgagccagcc ccagaaccgc gaccttcgcc
    26521 acatgaccgt tttcacgcac gttgtcgcag gacatcaccg taaacgcttt cagccctttt
    26581 tcacgacgca gacgcagggc ttctacgatg taaccaatcg cggacttcgg cgcagtcggg
    26641 ttttccagat cgtgcttaat cagcggatta ttgagatcca gctgaccgct tgccgcatcg
    26701 gcgcaataac ctttctctgt gaccgtcagg gagacaatcg ccgtctgcgg acgcgccatc
    26761 gcgttgagaa taccttcaca accgtcgatt tccgggtgca gggcttcttt catcgaaccg
    26821 ataattttca gctcggtgct ttctgcgcct ttttccgcca cggtgtacag cagttgctgt
    26881 tttttcaggt tttcgatcag cacgcggtcg ttacctggca tcaggttaac ttcgcagata
    26941 ccccagtcgc tgtcggtgct ttccagcaga tgatgggtgt acagcgcctg gtgcgcgcgg
    27001 tgaaacgccc cgcaaccgag atgcacaatg cgtgattcca gacgagaa
//
LOCUS       DAMZFT010000061        26468 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.061, whole genome shotgun sequence.
ACCESSION   DAMZFT010000061 DAMZFT010000000
VERSION     DAMZFT010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 26468)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26468)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26468
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.061"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(<1..345)
                     /locus_tag="PN977_002657"
     CDS             complement(<1..345)
                     /locus_tag="PN977_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HDI6042676.1"
                     /translation="MSQALKNLLTLLNLEKIEEGLFRGQSEDLGLRQVFGGQVVGQAL
                     YAAKETVPEERLVHSFHSYFLRPGDSKKPIIYDVETLRDGNSFSARRVAAIQNGKPIF
                     YMTASFQAPEAGF"
     gene            563..1135
                     /locus_tag="PN977_002658"
     CDS             563..1135
                     /locus_tag="PN977_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaY family lipoprotein"
                     /protein_id="HDI6042677.1"
                     /translation="MKLVHMASGLAVAIALAACADKSADIQTPAPAANTSISATQQPA
                     IQQPNVSGTVWIRQKVALPPDAVLTVTLSDASLADAPSKVLAQKAVRTEGKQSPFSFV
                     LPFNPADVQPNARILLSAAITVNDKLVFITDTVQPVINQGGTKADLTLVPVQQTAVPV
                     QASGGATTTVPSTSPTQVNPSSAVPAPTQY"
     gene            complement(1166..1477)
                     /locus_tag="PN977_002659"
     CDS             complement(1166..1477)
                     /locus_tag="PN977_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HDI6042678.1"
                     /translation="MEKEDSFPQRVWQIVAAIPEGYVTTYGDVAKLAGSPRAARQVGG
                     VLKRLPEGSTLPWHRVVNRHGTISLTGPDLQRQRQALLAEGVMVSGSGQIDLQRYRWN
                     Y"
     gene            1640..1736
                     /gene="ffs"
                     /locus_tag="PN977_002660"
     ncRNA           1640..1736
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="PN977_002660"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            1856..2209
                     /locus_tag="PN977_002661"
     CDS             1856..2209
                     /locus_tag="PN977_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1428 domain-containing protein"
                     /protein_id="HDI6042679.1"
                     /translation="MKYVDGFVVAVPADKKDAYREMAAKAAPLFKEFGALRIVECWAS
                     DVPDGKVTDFRMAVKAEENEEVVFSWIEYPSKEVRDAANQKMMSDPRMKEFGESMPFD
                     GKRMIYGGFESIIDE"
     gene            complement(2250..3800)
                     /gene="pdeB"
                     /locus_tag="PN977_002662"
     CDS             complement(2250..3800)
                     /gene="pdeB"
                     /locus_tag="PN977_002662"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414990.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeB"
                     /protein_id="HDI6042680.1"
                     /translation="MRTRHLVGLISGVLILSVLLPVGLSIWLAHQQVETSFIEELNTY
                     SSRVAIRANKVATQGKDALQELERWQGAACSEAHLMEMRRVSYSYRYIQEVVYIDNNV
                     PQCSSLEHESPPDTFPEPGKISKDGYRVWLTSHNDLGIIRYMVAMGTAHYVVMIDPAS
                     FIDVIPYSSWQIDAAIIGNAHNVVITSSDEIAQGIITRLQKTPGEHIENNGIIYDILP
                     LPEMNISIITWASTKMLQKGWHRQVFIWLPFGLVIGLLAAMFVLRILRRIQSPHHRLQ
                     DAIENRDICVHYQPIVSLANGKIVGAEALARWPQTDGSWLSPDSFIPLAQQTGLSEPL
                     TLLIIKSVFEDMGDWLRQHPQQHISINLESTVLTSEKIPQLLREMINHYQVNPRQIAL
                     ELTEREFADPKTSAPIISRYREAGHEIYLDDFGTGYSSLSYLQDLDVDILKIDKSFVD
                     ALEYKNVTPHIIEMAKTLKLKMVAEGIETSKQEEWLRQHSVHYGQGWLYSKALPKEDF
                     LRWAEQHL"
     gene            complement(3964..4434)
                     /locus_tag="PN977_002663"
     CDS             complement(3964..4434)
                     /locus_tag="PN977_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaC family protein"
                     /protein_id="HDI6042681.1"
                     /translation="MTEIQRLLTETIESLNTREKRDNKPRFSISFIRKHPGLFIGMYV
                     AFFATLAVMLQSETLSGSVWLLVVLFILLNGFFFFDVYPRYRYEDIDVLDFRVCYNGE
                     WYNTRFVPAALVEAILNSPRVADVHKEQLQKMIVRKGELSFYDIFTLARAESTS"
     gene            complement(4550..5101)
                     /gene="maa"
                     /locus_tag="PN977_002664"
     CDS             complement(4550..5101)
                     /gene="maa"
                     /locus_tag="PN977_002664"
                     /EC_number="2.3.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose O-acetyltransferase"
                     /protein_id="HDI6042682.1"
                     /translation="MSTEKEKMIAGELYRSADETLSRDRLRARQLIHRYNHSLAEEHT
                     LRQQILADLFGQVTEAYIEPTFRCDYGYNIFLGNNFFANFDCVMLDVCPIRIGDNCML
                     APGVHIYTATHPIDPVARNSGAELGKPVTIGNNVWIGGRAIINPGVTIGDNVVVASGA
                     VVTKDVPDNVVVGGNPARIIKKL"
     gene            complement(5273..5491)
                     /gene="hha"
                     /locus_tag="PN977_002665"
     CDS             complement(5273..5491)
                     /gene="hha"
                     /locus_tag="PN977_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin expression modulator Hha"
                     /protein_id="HDI6042683.1"
                     /translation="MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFY
                     SAADHRLAELTMNKLYDKIPSSVWKFIR"
     gene            complement(5517..5891)
                     /gene="tomB"
                     /locus_tag="PN977_002666"
     CDS             complement(5517..5891)
                     /gene="tomB"
                     /locus_tag="PN977_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hha toxicity modulator TomB"
                     /protein_id="HDI6042684.1"
                     /translation="MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAI
                     NLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYLDDTFMLFSSYGINMQDLQKWRK
                     SGNRLFRCFVNATKENPASLSC"
     gene            complement(6437..9586)
                     /locus_tag="PN977_002667"
     CDS             complement(6437..9586)
                     /locus_tag="PN977_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit"
                     /protein_id="HDI6042685.1"
                     /translation="MPNFFIDRPIFAWVIAIIIMLAGGLAILKLPVAQYPTIAPPAVT
                     ISASYPGADAKTVQDTVTQVIEQNMNGIDNLMYMSSNSDSTGTVQITLTFESGTDADI
                     AQVQVQNKLQLAMPLLPQEVQQQGVSVEKSSSSFLMVVGVINTDGTMTQEDISDYVAA
                     NMKDAISRTSGVGDVQLFGSQYAMRIWMNPNELNKFQLTPVDVITAIKAQNAQVAAGQ
                     LGGTPPVKGQQLNASIIAQTRLTSTEEFGKILLKVNQDGSRVLLRDVAKIELGGENYD
                     IIAEFNGQPASGLGIKLATGANALDTAAAIRAELAKMEPFFPSGLKIVYPYDTTPFVK
                     ISIHEVVKTLVEAIILVFLVMYLFLQNFRATLIPTIAVPVVLLGTFAVLAAFGFSINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVMAEEGLPPKEATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGSTGAIYRQFSITIVSAMALSVLVALILTPALCATMLKPIAKGDHGEG
                     KKGFFGWFNRMFEKSTHHYTDSVGGILRSTGRYLVLYLIIVVGMAYLFVRLPSSFLPD
                     EDQGVFMTMVQLPAGATQERTQKVLNEVTHYYLTKEKNNVESVFAVNGFGFAGRGQNT
                     GIAFVSLKDWADRPGEENKVEAITMRATRAFSQIKDAMVFAFNLPAIVELGTATGFDF
                     ELIDQAGLGHEKLTQARNQLLAEAAKHPDMLTSVRPNGLEDTPQFKIDIDQEKAQALG
                     VSINDINTTLGAAWGGSYVNDFIDRGRVKKVYVMSEAKYRMLPDDIGDWYVRAADGQM
                     VPFSAFSSSRWEYGSPRLERYNGLPSMEILGQAAPGKSTGEAMELMEQLASKLPTGVG
                     YDWTGMSYQERLSGNQAPSLYAISLIVVFLCLAALYESWSIPFSVMLVVPLGVIGALL
                     AATFRGLTNDVYFQVGLLTTIGLSAKNAILIVEFAKDLMDKEGKGLIEATLDAVRMRL
                     RPILMTSLAFILGVMPLVISTGAGSGAQNAVGTGVMGGMVTATVLAIFFVPVFFVVVR
                     RRFSRKNEDIEHSHTVDHH"
     gene            complement(9609..10802)
                     /gene="acrA"
                     /locus_tag="PN977_002668"
     CDS             complement(9609..10802)
                     /gene="acrA"
                     /locus_tag="PN977_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit AcrA"
                     /protein_id="HDI6042686.1"
                     /translation="MNKNRGFTPLAVVLMLSGSLALTGCDDKQAQQGGQQMPAVGVVT
                     VKTEPLQITTELPGRTSAYRIAEVRPQVSGIILKRNFKEGSDIEAGVSLYQIDPATYQ
                     AAYDSAKGDLAKAQAAANIAQLTVNRYQKLLGTQYISKQEYDQALADAQQANAAVTAA
                     KAAVETARINLAYTKVTSPISGRIGKSNVTEGALVQNGQATALATVQQLDPIYVDVTQ
                     SSNDFLRLKQELANGTLKQENGKAKVSLITSDGIKFPQDGTLEFSDVTVDQTTGSITL
                     RAIFPNPDHTLLPGMFVRARLEEGLNPNAILVPQQGVTRTPRGDATVLVVGADDKVET
                     RPIVASQAIGDKWLVTEGLKAGDRVVISGLQKVRPGVQVKAQEVTADNNQQAASGAQP
                     EQSKS"
     gene            10944..11591
                     /gene="acrR"
                     /locus_tag="PN977_002669"
     CDS             10944..11591
                     /gene="acrR"
                     /locus_tag="PN977_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor AcrR"
                     /protein_id="HDI6042687.1"
                     /translation="MARKTKQEAQETRQHILDVALRLFSQQGVSSTSLGEIAKAAGVT
                     RGAIYWHFKDKSDLFSEIWELSESNIGELELEYQAKFPGDPLSVLREILIHVLESTVT
                     EERRRLLMEIIFHKCEFVGEMAVVQQAQRNLCLESYDRIEQTLKHCIEAKMLPADLMT
                     RRAAIIMRGYISGLMENWLFAPQSFDLKKEARDYVAILLEMYLLCPTLRNPATNE"
     gene            11719..15081
                     /gene="mscK"
                     /locus_tag="PN977_002670"
     CDS             11719..15081
                     /gene="mscK"
                     /locus_tag="PN977_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel MscK"
                     /protein_id="HDI6042688.1"
                     /translation="MTMFQYYKRSRHFVFSAFIAFVFVLLCQNTAFARASSNGDLPTK
                     ADLQAQLDSLNKQKDLSAQDKLVQQDLTDTLATLDKIDRVKEETVQLRQKVAEAPEKM
                     RQATAALTALSDVDNDEETRKILSTLSLRQLETRVAQALDDLQNAQNDLASYNSQLVS
                     LQTQPERVQNAMYNASQQLQQIRSRLDGTDVGETALRPSQKVLMQAQQALLNAEIDQQ
                     RKSLEGNTVLQDTLQKQRDYVTANSARLEHQLQLLQEAVNSKRLTLTEQTAQEAVSPD
                     EAARIQANPLVKQELEINQQLSQRLITATENGNQLMQQNIKVKNWLERALQSERNIKE
                     QIAVLKGSLLLSRILYQQQQTLPSADELENMTNRIADLRLEQFEVNQQRDALFQSDAF
                     VNKLEEGHTNEVNSEVHDALLQVVDMRRELLDQLNKQLGNQLMMAINLQINQQQLMSV
                     SKNLKSILTQQIFWVNSNRPMDWNWIKAFPQSLKDEFKSMKITVNWEKAWPAVFIAFL
                     AGLPLLLIAGLIHWRLGWLKAYQQKLASAVGSLRNDSQLNTPKAILIDLIRALPVCLI
                     ILAVGLILLTMQLNISELLWSFSKKLAIFWLVFGLCWKVLEKNGVAVRHFGMPEQQTS
                     HWRRQIVRISLALLPIHFWSVVAELSPLHLMDDVLGQAMIFFNLLLIAFLVWPMCRES
                     WRDKESHTMRLVTITVLSIIPIALMVLTATGYFYTTLRLSGRWIETVYLVIIWNLLYQ
                     TVLRGLSVAARRIAWRRALARRQNLVKEGAEGAEPPEEPTIALEQVNQQTLRITMLLM
                     FALFGVMFWAIWSDLITVFSYLDSITLWHYNGTEAGAAVVKNVTMGSLLFAIIASMVA
                     WALIRNLPGLLEVLVLSRLNMRQGASYAITTILNYIIIAVGAMTVFGSLGVSWDKLQW
                     LAAALSVGLGFGLQEIFGNFVSGLIILFERPVRIGDTVTIGSFSGTVSKIRIRATTIT
                     DFDRKEVIIPNKAFVTERLINWSLTDTTTRLVIRLGVAYGSDLEKVRKVLLKAATEHP
                     RVMHEPMPEVFFTAFGASTLDHELRLYVRELRDRSRTVDELNRTIDQLCRENDINIAF
                     NQLEVHLHNEKGDEVTEVKRDYKGDDPTPAVG"
     gene            complement(15293..15454)
                     /gene="rsmS"
                     /locus_tag="PN977_002671"
     CDS             complement(15293..15454)
                     /gene="rsmS"
                     /locus_tag="PN977_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pleiotropic regulatory protein RsmS"
                     /protein_id="HDI6042689.1"
                     /translation="MSLENAPDDVKLAVDLIVLLEENQIPARTVLRALDIVKRDYEKK
                     LQSDDEAEK"
     gene            complement(15468..15995)
                     /gene="priC"
                     /locus_tag="PN977_002672"
     CDS             complement(15468..15995)
                     /gene="priC"
                     /locus_tag="PN977_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N''"
                     /protein_id="HDI6042690.1"
                     /translation="MKTALLLEKLEGQLATLRQRCAPVAQFATLSARFDRHLFQTRAT
                     TLQACLDEAGDNLSALRHAVEQQQLPQVAWLAEHLAAQLEAIAREATAWSLREWDSAP
                     PKIARWQRKRIQHQDFERRLREMVAERRARLARVTDLVEQQTLHREVEAYEARLARCR
                     HALDKIENRLARLTR"
     gene            16065..16442
                     /locus_tag="PN977_002673"
     CDS             16065..16442
                     /locus_tag="PN977_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF454 family protein"
                     /protein_id="HDI6042691.1"
                     /translation="MQRIILIIIGWLAVVLGTLGVVLPVLPTTPFILLAAWCFARSSP
                     RFHAWLLYRSWFGSYLRFWQKHHAMPRGVKPRAILLILLTFAISLWFVQMPWVRIMLL
                     VILACLLFYMWRIPVIDEKQEKH"
     gene            16595..17146
                     /gene="apt"
                     /locus_tag="PN977_002674"
     CDS             16595..17146
                     /gene="apt"
                     /locus_tag="PN977_002674"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HDI6042692.1"
                     /translation="MTATAQQLEYLKNSIKSIQDYPKPGILFRDVTSLLEDPKAYALS
                     IDLLVERYKNAGITKVVGTEARGFLFGAPVALGLGVGFVPVRKPGKLPRETISETYDL
                     EYGTDQLEIHVDAIKPGDKVLVVDDLLATGGTIEATVKLIRRLGGEVADAAFIINLFD
                     LGGEQRLEKQGITSYSLVPFPGH"
     gene            17275..19206
                     /gene="dnaX"
                     /locus_tag="PN977_002675"
     CDS             17275..19206
                     /gene="dnaX"
                     /locus_tag="PN977_002675"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HDI6042693.1"
                     /translation="MSYQVLARKWRPQTFADVVGQEHVLTALANGLSLGRIHHAYLFS
                     GTRGVGKTSIARLLAKGLNCETGITATPCGVCDNCREIEQGRFVDLIEIDAASRTKVE
                     DTRDLLDNVQYAPARGRFKVYLIDEVHMLSRHSFNALLKTLEEPPEHVKFLLATTDPQ
                     KLPVTILSRCLQFHLKALDVEQIRHQLEHILNEEHIAHEPRALQLLARAAEGSLRDAL
                     SLTDQAIASGDGQVSTQAVSAMLGTLDDDQALSLVEAMVEANGERVMALINEAAARGI
                     EWEALLVEMLGLLHRIAMVQLSPAALGNDMAAIELRMRELARTIPPTDIQLYYQTLLI
                     GRKELPYAPDRRMGVEMTLLRALAFHPRMPLPEPEVPRQSFAPVAPTAVMTPTQVPPQ
                     PQSAPQQAPTVPLPETTSQVLAARQQLQRVQGATKAKKSEPAAATRARPVNNAALERL
                     ASVTDRVQARPVPSALEKAPAKKEAYRWKATTPVMQQKEVVATPKALKKALEHEKTPE
                     LAAKLAAEAIERDPWAAQVSQLSLPKLVEQVALNAWKEESDNAVCLHLRSSQRHLNNR
                     GAQQKLAEALSTLKGSTVELTIVEDDNPAVRTPLEWRQAIYEEKLAQARESIIADNNI
                     QTLRRFFDAELDEESIRPI"
     misc_feature    18539..18603
                     /gene="dnaX"
                     /locus_tag="PN977_002675"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00382"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="DnaX frameshifting element; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00382"
     gene            19259..19588
                     /locus_tag="PN977_002676"
     CDS             19259..19588
                     /locus_tag="PN977_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001364632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HDI6042694.1"
                     /translation="MFGKGGLGNLMKQAQQMQEKMQKMQEEIAQLEVTGESGAGLVKV
                     TINGAHNCRRVEIDPSLLEDDKEMLEDLVAAAFNDAARRIEETQKEKMASVSSGMQLP
                     PGFKMPF"
     gene            19588..20193
                     /gene="recR"
                     /locus_tag="PN977_002677"
     CDS             19588..20193
                     /gene="recR"
                     /locus_tag="PN977_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HDI6042695.1"
                     /translation="MQTSPLLTQLMEALRCLPGVGPKSAQRMAFTLLQRDRSGGMRLA
                     QALTRAMSEIGHCADCRTFTEQEVCNICSNPRRQENGQICVVESPADIYAIEQTGQFS
                     GRYFVLMGHLSPLDGIGPDDIGLDRLEQRLAEEKITEVILATNPTVEGEATANYIAEL
                     CAQYDVEASRIAHGVPVGGELEMVDGTTLSHSLAGRHKIRF"
     gene            20303..22177
                     /gene="htpG"
                     /locus_tag="PN977_002678"
     CDS             20303..22177
                     /gene="htpG"
                     /locus_tag="PN977_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003903109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HDI6042696.1"
                     /translation="MKGQETRGFQSEVKQLLHLMIHSLYSNKEIFLRELISNASDAAD
                     KLRFRALSNPDLYEGDGELRVRVSFDKDKRTLTISDNGVGMTRDEVIDHLGTIAKSGT
                     KSFLESLGSDQAKDSQLIGQFGVGFYSAFIVADKVTVRTRAAGEKPENGVFWESAGEG
                     EYTVADITKEDRGTEITLHLREGEDEFLDDWRVRSIISKYSDHIALPVEIEKREEKDG
                     ETVISWEKINKAQALWTRNKSEITDEEYKEFYKHIAHDFNDPLTWSHNRVEGKQEYTS
                     LLYIPSQAPWDMWNRDHKHGLKLYVQRVFIMDDAEQFMPNYLRFVRGLIDSSDLPLNV
                     SREILQDSTVTRNLRNALTKRVLQMLEKLAKDDAEKYQTFWQQFGLVLKEGPAEDFAN
                     QEAIAKLLRFASTHTDSSAQTVSLEDYVSRMKEGQEKIYYITADSYAAAKSSPHLELL
                     RKKGIEVLLLSDRIDEWMMNYLTEFDGKPFQSVSKVDESLEKLADEVDESAKEAEKAL
                     TPFIDRVKALLGERVKDVRLTHRLTDTPAIVSTDADEMSTQMAKLFAAAGQKVPEVKY
                     IFELNPDHVLVKRAADTEDEAKFSEWVELLLDQALLAERGTLEDPNLFIRRMNQLLVS
                     "
     gene            22358..23002
                     /gene="adk"
                     /locus_tag="PN977_002679"
     CDS             22358..23002
                     /gene="adk"
                     /locus_tag="PN977_002679"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007706188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HDI6042697.1"
                     /translation="MRIILLGAPGAGKGTQAQFIMEKYGIPQISTGDMLRAAVKSGSE
                     LGKQAKDIMDAGKLVTDELVIALVKERIAQEDCRNGFLLDGFPRTIPQADAMKEAGIN
                     VDYVLEFDVPDELIVDRIVGRRVHAPSGRVYHVKFNPPKVEGKDDVTGEELTTRKDDQ
                     EETVRKRLVEYHQMTAPLIGYYSKEAEAGNTKYAKVDGTKPVAEVRADLEKILG"
     gene            23134..24096
                     /gene="hemH"
                     /locus_tag="PN977_002680"
     CDS             23134..24096
                     /gene="hemH"
                     /locus_tag="PN977_002680"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HDI6042698.1"
                     /translation="MRQTKTGILLANLGTPDAPTPEAVKRYLKQFLSDRRVVDTSRLL
                     WWPLLRGVILPLRSPRVAKLYASVWMEGGSPLMVYSRQQQQALAQRLPETPVALGMSY
                     GSPSLESAVDELLAEHVDHIVVLPLYPQYSCSTVGAVWDELARILARKRSIPGISFIR
                     DYADNHDYINALANSVRASFAKHGEPDLLLLSYHGIPQRYADEGDDYPQHCRTTTREL
                     ASALEMAPEKVMMTFQSRFGREPWLMPYTDETLKMLGEKGVGHIQVMCPGFAADCLET
                     LEEIAEQNREVFLGAGGKKYEYIPALNATPEHIEMMANLVAAYR"
     gene            complement(24093..25052)
                     /gene="aes"
                     /locus_tag="PN977_002681"
     CDS             complement(24093..25052)
                     /gene="aes"
                     /locus_tag="PN977_002681"
                     /EC_number="3.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl esterase"
                     /protein_id="HDI6042699.1"
                     /translation="MKPENKLPVLDLISAEMKTVVNTLQPDLPPWPATGTIAEQRQYY
                     TLERRFWNAGAPEMATRAYMVPTKYGQVETRLFCPQPDSPATLFYLHGGGFILGNLDT
                     HDRIMRLLASYSQCTVIGIDYTLSPEARFPQAIEEIVAACCYFHQQAEDYQINMSRIG
                     FAGDSAGAMLALASALWLRDKQIDCGKVAGVLLWYGLYGLRDSVTRRLLGGVWDGLTQ
                     QDLQMYEEAYLSNDADRESPYYCLFNNDLTREVPPCFIAGAEFDPLLDDSRLLYQTLA
                     AHQQPCEFKLYPGTLHAFLHYSRMMKTADEALRDGAQFFTAQL"
     gene            25204..>26468
                     /gene="gsk"
                     /locus_tag="PN977_002682"
     CDS             25204..>26468
                     /gene="gsk"
                     /locus_tag="PN977_002682"
                     /EC_number="2.7.1.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/guanosine kinase"
                     /protein_id="HDI6042700.1"
                     /translation="MKFPGKRKSKHYFPVNARDPLLQQFQPENETSAAWVVGIDQTLV
                     DIEAKVDDEFIERYGLSAGHSLVIEDDVAEALYQELKQKNLITHQFAGGTIGNTMHNY
                     SVLADDRSVLLGVMCSNIEIGSYAYRYLCNTSSRTDLNYLQGVDGPIGRCFTLIGESG
                     ERTFAISPGHMNQLRAESIPEDVIAGASALVLTSYLVRCKPGEPMPEATMKAIEYAKK
                     YNVPVVLTLGTKFVIAENPQWWQQFLKDHVSILAMNEDEAEALTGESDPLLASDKALD
                     WVDLVLCTAGPIGLYMAGFTEDEAKRKTQHPLLPGAIAEFNQYEFSRAMRHKDCQNPL
                     RVYSHIAPYMGGPEKIMNTNGAGDGALAALLHDITANSYHRSNVPNSSKHKFTWLTYS
                     SLAQVCKYANRVSYQVLNQHSPRLTRGLP"
BASE COUNT         6543 a         6785 c         6842 g         6298 t
ORIGIN      
        1 gaaacccgct tctggtgcct ggaaagaggc cgtcatataa aaaatcggtt tgccgttttg
       61 aatagcagca acccggcggg cgctgaagct gttaccgtca cgcagcgttt cgacatcata
      121 aataatcggt ttcttactat cgcctgggcg aagaaagtag ctgtgaaacg aatgtaccag
      181 ccgctcttca gggacggtct cttttgcagc atacaaggcc tgacccacga cctggccgcc
      241 aaacacctgg cgtaaaccta aatcttcact ctggccgcga aagagtcctt cctcaatttt
      301 ttccagattt aacaatgtca gtaaattttt tagcgcctga ctcatataac tctccagtaa
      361 caaagctgcc gcagcaagcc aaagtgagtt gagtataacg caaatttgcc actggtccga
      421 tgggtgcaat ggtctgaatt acgggctaat tacaggcaga aatgcgtgat gtgtgccaca
      481 cttgttgacg ttactatttt gttaaccact cttccggcga ggaaagttag cccgctggtg
      541 cattgataat aaggagaaga gaatgaaact cgtgcacatg gccagtggtt tagcggttgc
      601 gattgcgttg gcggcttgcg cagataaaag cgcggatatt cagacgccag caccggctgc
      661 aaatacgtct atttcagcaa cacaacaacc tgctatccag caaccgaatg tctccggtac
      721 cgtttggatc cgtcagaaag tcgcactgcc gcctgatgcc gtgctgaccg tgacactttc
      781 tgacgcgtcg ttagccgatg caccgtcaaa agtgctggcg cagaaagcgg tgcgtaccga
      841 aggtaaacag tcaccattca gctttgttct gccatttaac ccggcagatg ttcagccgaa
      901 tgcgcgtatt ctgttgagtg cggcgattac cgtgaatgac aaactggtat ttattaccga
      961 taccgttcag ccggtgatca accagggcgg aactaaagcc gacctgacat tggtgccggt
     1021 gcagcaaacc gccgtgccgg ttcaggccag cggtggcgca acgactaccg taccttcgac
     1081 ttcaccaact caggtgaatc cgtcttcggc agttccagct cctacgcaat attaagcgca
     1141 gttaaccctc tccgcccgga gagggttagt agttccagcg ataacgctgc aagtcgattt
     1201 gcccgcttcc cgataccatc acaccttctg ccagtaatgc ctgtcgttga cgctgtaaat
     1261 ccggtccggt tagcgaaatt gtgccgtggc gattaaccac ccggtgccag ggcaaggtgc
     1321 tgccttcagg gagacgcttt aacacaccgc ccacctggcg cgcggcgcgg ggcgatcctg
     1381 ccagtttcgc cacatcaccg taagtggtga catagccttc gggaatagcg gcgacgattt
     1441 gccagacgcg ttggggaaat gaatcttctt tttccatctt ttcttcctga ggtaattttt
     1501 cagcataatc tggaaaaacg cccgagtgaa gtcgcattgc gcaagaaacc agcatccggc
     1561 acgcaatggg ttgcaattag ctggggcagc agtgataatg cgcctgcgcg ttggttctca
     1621 acgctctcaa tgggggctct gttggttctc ccgcaacgct actctgttta ccaggtcagg
     1681 tccggaagga agcagccaag gcagatgacg cgtgtgccgg gatgtagctg gcagggcccc
     1741 cacccatttc tgcctcccac cgtttcgtca aaaaatccca acatggctaa actttaacca
     1801 caactgacgt cgcaagaatt gtctggctgc gcagtacgct tcggaggtat gtctgatgaa
     1861 gtatgttgat ggttttgttg ttgccgttcc tgccgataaa aaggatgcct atcgggaaat
     1921 ggccgctaag gccgcgccat tgtttaaaga gtttggcgcg cttcgtattg tcgaatgctg
     1981 ggccagcgat gtaccggatg gcaaagtgac cgattttcgt atggcggtaa aagctgaaga
     2041 gaatgaagag gttgtcttta gctggattga atacccttca aaagaggtcc gcgacgctgc
     2101 taatcaaaag atgatgtcgg acccacggat gaaagagttc ggcgagtcca tgccgtttga
     2161 cggcaagcga atgatctatg gtggattcga atcaatcatc gacgagtagg tagcggacgg
     2221 gctgtgcggc gtgccgtcag ccctaaaatt cacaaatgtt gctcggccca gcgtaagaaa
     2281 tcctctttcg gtaatgcctt gctgtagagc cagccctggc cgtagtgcac gctatgctga
     2341 cgtagccact cttcttgttt actggtttcg attccctccg ctaccatttt cagtttcagt
     2401 gtttttgcca tttcgatgat atgtggcgtg acatttttat attccagcgc atcaacgaaa
     2461 gatttatcga tcttcagaat gtcgacatcc agatcctgta aatagcttaa acttgaatac
     2521 cccgtaccaa aatcatcgag ataaatttca tggcccgcct cccggtagcg agaaattatc
     2581 ggggcgctgg ttttcggatc ggcaaactcg cgttcagtga gttcaagcgc gatctgtctg
     2641 ggattaacct gatagtgatt gatcatttca cgcagcaatt gcgggatttt ttccgaggtg
     2701 agcacggtgg attcaagatt gatcgaaata tgctgctgcg gatgctgacg cagccagtcg
     2761 cccatatctt caaagacact ttttataatc agtagcgtca acggctcaga aagccccgtt
     2821 tgctgtgcca gcggaataaa actatctggt gacaaccaac taccgtctgt ctgcggccag
     2881 cgcgccagtg cctcagcacc gacaattttg ccattggcta aggagacaat cggctgatag
     2941 tgcacgcaaa tatcacgatt ttcgatagca tcctgcagcc gatgatgcgg tgactggata
     3001 cggcgcaaaa tacgcagcac aaacatcgct gccagcaggc caatcaccaa cccgaacggt
     3061 aaccaaataa agacttgccg atgccagcct ttctgcaaca ttttcgttga agcccaggtg
     3121 atgatcgaaa tattcatctc cggtaagggc aggatatcgt aaatgattcc attattttcg
     3181 atatgctcac cgggtgtttt ttgtagtctg gtaataattc cctgagcaat ttcatcactg
     3241 ctggttatca caacgttatg ggcattgcca ataatggcgg catcaatttg ccatgagcta
     3301 tagggaatga catcaataaa ggaagcgggg tcgatcatga cgacataatg tgccgttccc
     3361 atggcgacca tgtaacggat aatgcctaaa tcgttatgcg atgttaacca gacacgataa
     3421 ccatctttcg aaattttacc tggctcgggg aaggtatcgg gcgggctttc atgctccaga
     3481 gacgaacact ggggaacgtt gttatcgata taaaccactt cctgaatata gcgataactg
     3541 taagataccc gacgcatttc catgagatgg gcttcgctac aggcagcgcc ttgccatctt
     3601 tccagctctt gcagcgcatc tttcccttgt gtcgccacct tattggcccg aatagcgacg
     3661 cgggaggaat acgtattcag ctcttcaata aatgatgttt ctacctgctg atgggccagc
     3721 cagatgctta agccgacagg cagcaatact gaaagaatca gtactcccga aataaggccg
     3781 accagatgtc gtgttctcac cagcatatcc ttattaacac agtgtaggga aagtatatct
     3841 gattgtggaa aaaggctggc aggagataag gaggtagagc cgggatttca tgaagtgatg
     3901 ggtggaaacg aacgttgtca tttgaccagg cgcagagctt tacgcctggt atgtaaccct
     3961 aacttaagat gttgattcgg cgcgagcgag ggtaaaaata tcgtaaaaag acagttcacc
     4021 tttacggacg atcatttttt gcagttgttc tttatgaaca tccgcgacac gcggagagtt
     4081 caagatggct tcaaccagcg cggcaggtac aaagcgcgtg ttgtaccatt cgccgttata
     4141 gcaaacgcgg aaatccagca cgtcgatatc ttcatagcgg tagcgtgggt agacatcgaa
     4201 aaagaagaaa ccatttagca ggataaataa taccaccagt agccagacag agcctgaaag
     4261 cgtttcggac tgcaacatca ccgccagggt ggcaaaaaaa gcaacataca taccgataaa
     4321 tagccccgga tgtttacgga taaaactgat actaaagcgg ggtttgttgt cgcgtttttc
     4381 gcgggtattc agagactcaa tcgtttcggt cagcaggcgt tgtatttcgg tcatcgtttg
     4441 ccttcgtggt atctgcaact ttccagggaa caccctattt taacgtggga atagtagagg
     4501 gaaagtaaca gatttgccac gattttgcat aacagttgcg aaaaactggt tacaattttt
     4561 taattattct ggctggatta ccgcccacga caacgttgtc cgggacatct tttgtgacaa
     4621 ctgcacctga ggctaccacg acgttatcac caatggtcac accagggtta atgatcgcgc
     4681 gtccgccaat ccagacgtta ttaccgatgg tgacgggttt ccccagttca gcaccgctat
     4741 tacgtgctac agggtcgatg ggatgtgttg ccgtgtagat atgaacgcct ggtgccaaca
     4801 tacagttatc accgatgcga atagggcaga catcaagcat cacgcaatcg aagttggcga
     4861 aaaaattatt gccgagaaaa atgttatagc cataatcaca gcgaaacgtt ggctcaatat
     4921 aagcctctgt cacctgaccg aataaatcag cgagaatttg ctggcgtaat gtgtgctctt
     4981 ccgccaggga atgattgtat cggtgaataa gctgacgagc gcgcaggcga tcgcgagata
     5041 acgtctcatc tgccgagcga tacaactcac cagcaatcat cttttctttt tctgtgctca
     5101 ttactgacct ccggttgctg aaaatgcggc aacacagtaa gacgaaaagt cagtgaagag
     5161 aacgttccca ttacgtctgt gtgatgaaga tcacataggg tatgccttca tggcgataaa
     5221 gtataaaatt cttaataaac agctggttat agctccgaaa gccaattatt tattagcgaa
     5281 taaatttcca tactgaggaa gggatcttgt cgtacagttt attcatagtc aattcggcga
     5341 ggcggtgatc tgcggctgag taaaataccg ccagttcatt atctgataat tcgtatttat
     5401 ttttctcgat aacacgctcc agcgtgtcaa ttgtctggca acgacgtaaa cgcattaaat
     5461 aatcggtttt cgttaaaggt ttttcggaca tacttctacc catggttgta ataattctaa
     5521 caagataaac tcgcaggatt ctctttcgtc gcattgacaa aacaacggaa tagtcgatta
     5581 cctgacttcc gccatttttg gagatcctgc atattaatac cataactgct gaacaacata
     5641 aaggtgtcat ccagatattc gtcgatctgc tcaatgagct tattgtcttc attatactta
     5701 attttgtaat taagtgcgaa agtcgcaata tgctcaatca gttcattcaa ctggaggttg
     5761 atcgccgagg ttgggtcgtt aacccagcca tgattgcttt cttcaaggtt tgcaagacag
     5821 tcatgataca gggtttcaca gagaaactta agctgtgcga tatcatgtct tttgggtgag
     5881 tattcatcca taactcgtcc ccttcttagc ggttgaacta acggacacct ttcgggatgg
     5941 aaaaaactta ctgacctgga cttgccctct cgtttgttag cttaactata agccactctt
     6001 tgcaggtttt catcgcatta ttacgaaaaa ttacaattag gaaggaagcg ggacaggact
     6061 tactttttca tactagcact aacccgcagc aggtaaaagc agtttgctcg aactgttcat
     6121 aacatttcag gattatcgcc agatgaaatt ggtaacacta ataccaggat tgctctgaat
     6181 atgacgtaat aaccgaggaa tgaataaaga attaccgcaa ataattaaga atagcctcta
     6241 aatgattatg gattattaaa tctattagcg ctacttaata taaattaact aacaatcagt
     6301 attttttatg aattttatac gtgtttaata ctggttttcg tatgagatcc tgagttggtg
     6361 gttcaatgac tccttaatgt tcgtaggtta tgcataaaaa aggccgcttg cgcggcctta
     6421 gtgattacac gttgtatcaa tgatgatcga cagtatggct gtgctcgata tcttcattct
     6481 tgcggctaaa gcggcggcga accaccacaa agaataccgg aacgaagaag attgccagta
     6541 ccgttgcggt caccatcccg cccattacac cggtacctac tgcgttctgc gcgccggaac
     6601 cagcaccagt actgataacc agcggcataa cgccgaggat aaacgccagc gaggtcatca
     6661 ggattgggcg taaacgcatc cgcaccgcat caagcgtcgc ttcaatcaga cctttacctt
     6721 ctttatccat caagtctttg gcgaattcga cgataagtat cgcgttcttc gccgacaacc
     6781 caatggttgt gagcaggcct acctggaagt aaacgtcatt ggtcaggcca cggaaggtgg
     6841 cagccagcaa cgcaccgata acccccagcg gaacgaccag cataacggag aacggaatcg
     6901 accagctctc gtacagcgcc gccagacaca ggaacacgac aatcaacgaa atcgcgtaca
     6961 gtgaaggtgc ctggttgccg gagagacgtt cctgatagga catccccgtc cagtcatagc
     7021 caacaccggt aggcagtttg ctcgccagtt gttccatcag ctccattgct tcaccggtac
     7081 ttttacccgg tgccgcctgg cctaagattt ccatggatgg caggccgttg taacgttcca
     7141 gacgcggcga accgtactcc caacgagaag aggagaacgc cgagaacggc accatctgac
     7201 catcagcagc acgaacatac cagtcgccga tatcatccgg cagcatacgg tatttcgctt
     7261 ctgacatgac gtaaactttc ttcacacgac cgcggtcgat aaagtcgttc acatagctac
     7321 cgccccatgc agcgcccaga gtggtgttaa tgtcgttgat agaaacaccc agcgcctgcg
     7381 ctttttcctg gtcgatatca atcttaaact gcggggtatc ttccagaccg tttggacgta
     7441 cgctggtcaa catatcaggg tgcttcgctg cttctgcaag cagctggtta cgcgcctgag
     7501 tcagtttttc gtgaccaagg ccagcctggt caatcagctc aaagtcaaag ccggttgcag
     7561 tacccagttc cacgattgcg ggcaggttaa aggcgaaaac catcgcatct ttgatttgcg
     7621 agaaagcgcg tgttgcacgc atggtaatcg cttcaacttt gttttcttcg cccggacgat
     7681 cggcccagtc cttcaaggaa acgaacgcaa taccggtatt ctgaccacgt cccgcaaagc
     7741 cgaagccgtt aacggcgaac accgactcaa cgttgttctt ttctttggtc agatagtaat
     7801 gcgttacctc attgagcact ttctgcgtac gttcctgcgt tgcacctgct ggcagctgaa
     7861 ccatggtcat aaatacgccc tggtcttcat ctggcaagaa ggagcttggc agacgcacga
     7921 acagataggc catgccgacc acgatgatca gatacagcac caggtaacgc cctgtactgc
     7981 gcagaatacc gcctacgctg tcggtgtagt ggtgcgtgct cttttcgaac atgcggttaa
     8041 accagccgaa gaagcctttt ttaccttccc cgtgatcgcc tttggcaatc ggtttcagca
     8101 tggtggcaca aagagccgga gtcaggatca acgccaccag tactgacagc gccattgctg
     8161 aaacaatggt aatagagaac tgacgataaa tagcaccggt agaaccgcca aagaaggcca
     8221 tcggtacgaa taccgccgac agtaccatcg cgataccgac cagagcgccc tgaatctgcc
     8281 ccatcgactt acgggtagct tcttttggcg gcaaaccttc ttccgccata acacgctcaa
     8341 cgttttctac cacaacgatg gcgtcatcca ccaacaggcc gatggcgagc accatcccga
     8401 acattgttag cgtgtttatc gagaagccaa aggcggcaag gacggcaaag gtccccagca
     8461 ataccaccgg tacggcaatg gtcggaatca acgtcgcgcg gaagttctgc aggaacagat
     8521 acataaccag gaacacgagg atgatcgctt cgaccagcgt tttaaccact tcgtgaatag
     8581 agattttcac gaacggcgtg gtgtcgtacg ggtaaacaat tttcagaccc gacgggaaga
     8641 acggttccat cttcgccagt tcagcacgga ttgccgcagc ggtatccagc gcgtttgcac
     8701 cggtcgccag cttgatcccc agaccggaag ccggttggcc gttaaactct gcgatgatgt
     8761 cgtagttctc accacccagc tcaattttcg ccacatcacg cagcagcacg cgggaaccat
     8821 cctgattcac tttcagcagg attttgccga actcttcagt agaggtcaga cgcgtctgag
     8881 caataataga ggcgttaagc tgttggcctt tcaccggcgg cgtaccaccg agctgaccag
     8941 ctgcaacctg ggcgttctgt gctttgatgg cggtaatgac atcaaccggc gttagctgga
     9001 atttgttcag ttcattcggg ttcatccaga tacgcatcgc gtactgtgaa ccgaacaact
     9061 gaacgtcacc cacgcccgac gtacggctga tggcatcttt catattcgcc gccacgtagt
     9121 cggagatatc ctcctgcgtc atggtgccat cggtgttgat aacgccgaca accatcagga
     9181 agctgctgga tgatttctca acgctcaccc cttgctgctg aacttcttgc ggcagcaacg
     9241 gcatcgccag ctgcagtttg ttctgcacct gaacctgcgc gatatccgca tcagtaccag
     9301 actcaaaggt cagggtgatc tgcacggtac ccgtggagtc actgttagag gacatgtaca
     9361 tcaggttatc gataccgttc atattctgtt cgataacctg tgtcaccgtg tcctgcactg
     9421 ttttcgcatc agcgccaggg taggaggcgg agatcgttac tgccggcggt gcaatcgtag
     9481 gatattgcgc caccggcagt ttgaggatcg ccagcccccc tgccaacatg atgataatgg
     9541 cgatcaccca cgcaaaaatc gggcgatcga taaagaaatt aggcatgtct taacggctcc
     9601 tgtttaagtt aagacttgga ctgttcaggc tgagcaccgc ttgcggcttg ctggttatta
     9661 tcagcggtaa cttcttgtgc ttttacctgg acaccaggac gcactttctg cagcccactt
     9721 attactacgc gatcgcctgc tttcagacct tctgtcacca gccacttatc gccgatagcc
     9781 tggcttgcaa cgatcggacg ggtttccact ttgtcatccg cgccaaccac cagtacggtg
     9841 gcatcgccac gcggcgtacg ggttacgccc tgttgcggga ctaaaatagc gtttggatta
     9901 agcccttctt ccagacgcgc acgtacgaac atacccggca gcagagtgtg atccgggttc
     9961 gggaagatag cgcgtagggt gatagacccg gtggtctgat caacggtaac gtcagagaat
    10021 tccagcgtac cgtcctgcgg gaacttaatg ccgtcactgg tgatcagcga cactttggct
    10081 ttgccgttct cttgtttcag cgtgccattt gccagttcct gtttcaggcg caggaagtcg
    10141 ttgctggact gggtcacatc aacgtagatc ggatcaagtt gctgcacggt tgccagcgca
    10201 gtcgcctgac cgttctgtac caatgcgcct tctgtcacgt tcgacttacc aatgcgaccg
    10261 ctaatcggcg aggtgacttt ggtgtaagcc agattgatcc gcgcagtttc aacggcagct
    10321 ttcgccgcag tgaccgcagc attcgcctgt tgcgcatcag ccagagcctg atcgtactct
    10381 tgcttactga tgtactgagt accgagcaat ttctgataac gattcaccgt caattgcgcg
    10441 atattggctg cagcctgggc tttcgccaga tcacctttcg cactgtcgta tgccgcctga
    10501 taggtcgcag gatcaatctg atagagagag acacctgctt cgatgtcgct accttctttg
    10561 aaattacgct tcaggataat cccgctaact tgaggacgaa cttctgcgat ccggtaggca
    10621 ctggtgcgac ccggaagctc ggttgtgatc tgcagaggtt cagttttgac tgttactacg
    10681 ccaacggcgg gcatctgctg gccaccttgt tgggcctgtt tgtcgtcaca tcctgttagg
    10741 gctaagctgc ctgagagcat cagaacgacc gccagaggcg taaaccctct gtttttgttc
    10801 atatgtaaac ctcgagtgtc cgatttcaaa ttggtcaatg gtcaaaagtt aataaaccca
    10861 ttgctgcgtt tatattatcg tcgtgctatg gtacatacat tcacaaatgt atgtaaatct
    10921 aacgcctgta aattcacgaa catatggcac gaaaaaccaa acaagaagcg caagaaacgc
    10981 gccaacacat cctcgatgtg gctctacgtc ttttctcaca gcagggggta tcatccacct
    11041 cgctgggcga gattgcaaaa gcagctggcg ttacgcgcgg tgcaatctac tggcatttta
    11101 aagacaagtc ggatttgttc agtgagatct gggaactgtc agaatccaat attggtgaac
    11161 tagagcttga gtatcaggca aaattccctg gcgatccact ctcagtatta agagagatat
    11221 taattcatgt tcttgaatcc acggtgacag aagaacggcg tcgattattg atggagatta
    11281 tattccacaa atgcgaattt gtcggagaaa tggctgttgt gcaacaggca caacgtaatc
    11341 tctgtctgga aagttatgac cgtatagaac aaacgttaaa acattgtatt gaagcgaaaa
    11401 tgttgcctgc ggatttaatg acgcgtcgcg cagcaattat tatgcgcggc tatatttccg
    11461 gcctgatgga aaactggctc tttgccccgc aatcttttga tcttaaaaaa gaagcccgcg
    11521 attacgtcgc catcttactg gagatgtatc tcctgtgccc cacgcttcgt aatcctgcca
    11581 ctaacgaata accctgaatc tgactccagg atttttcctg gacattttcg tcgttgctat
    11641 tctggttcac tgcgtcgtga tattcttgcg gtttgacttt ttcaggtcgt tcttcaggtt
    11701 cagaaacctt cattcatcat gactatgttc cagtattaca aacgatcacg gcattttgtt
    11761 ttttcagcat ttattgcttt tgtttttgtc ttgttatgcc agaacacggc gtttgcgcgg
    11821 gcgtcatcga atggtgatct gccgacaaaa gcggacctgc aggcacaact tgactcactg
    11881 aataaacaaa aagatctttc tgctcaggac aaactggtgc agcaggatct gacagataca
    11941 ttagccaccc tcgataaaat cgatcgcgta aaagaagaga cagttcagct acggcaaaaa
    12001 gtcgctgaag cgccggaaaa aatgcgtcag gcgaccgcag cgttaacagc acttagcgat
    12061 gtcgataatg acgaagaaac gcgcaaaatt ctgagcacgc tttcgttgcg ccagctggaa
    12121 actcgcgttg cccaggcgct ggacgatttg caaaacgcac aaaacgatct ggcgtcttat
    12181 aacagccagc tggtttcgtt acagacgcag cccgaacgcg tgcaaaatgc gatgtataac
    12241 gcttcgcagc agctgcaaca aattcgcagt cgtctggatg ggactgatgt cggcgagact
    12301 gccttacgtc ccagccagaa agtgttaatg caggcccagc aggcgttgct gaatgcggag
    12361 attgaccagc agcgtaaaag cctggaagga aacacagtct tacaggatac cttgcaaaag
    12421 caacgggatt acgtgacggc gaacagcgcc cgtctggagc accagttgca gctgttgcaa
    12481 gaagcggtaa acagcaagcg cctgacttta accgaacaaa cggcgcagga ggccgtctcc
    12541 ccggatgaag ccgcgcgtat tcaggctaat ccgctggtga agcaggaact ggaaattaac
    12601 cagcagttaa gtcagcgtct gattaccgcg actgaaaacg gtaatcagtt gatgcagcaa
    12661 aacattaaag tcaaaaactg gctggagcgg gcgctgcaat cggaacgcaa tattaaagag
    12721 cagattgccg tcctgaaggg cagcctgctg ttgtctcgta tcctttacca gcaacaacaa
    12781 acgctgccct cggcggatga actggaaaac atgaccaacc gcatcgcgga tttgcgtctc
    12841 gaacagtttg aagttaacca gcagcgtgat gcactcttcc agagcgatgc gttcgtcaac
    12901 aaactggaag aaggtcacac caacgaagtc aacagcgaag ttcacgatgc attattgcaa
    12961 gtggttgata tgcgtcgcga attgctggat cagcttaaca aacagttggg taaccaattg
    13021 atgatggcca ttaacctgca aatcaaccag cagcagttaa tgagtgtgtc gaaaaacctg
    13081 aaatccatcc tgactcagca aatcttttgg gtgaacagta accgtccaat ggactggaac
    13141 tggatcaaag cgttcccgca aagcctgaaa gatgaattta agtcgatgaa aatcacggtg
    13201 aactgggaaa aagcctggcc tgccgttttt atcgctttcc tcgctggttt gccgctgctg
    13261 ttgattgccg ggctgatcca ctggcgtctg ggctggctga aagcgtatca acaaaaactg
    13321 gcttccgctg tgggttccct gcgtaacgac agccagctca acacaccaaa agcgatcctt
    13381 atcgacctga tccgtgcgct gccggtgtgc ctgattattc tcgcggttgg cctgattctg
    13441 ttgaccatgc agctcaacat cagcgaactg ctatggtcgt tcagtaaaaa actggcgatt
    13501 ttctggctgg tgtttggcct gtgctggaaa gtgttggaga aaaacggcgt tgccgtgcgt
    13561 cacttcggca tgccggaaca gcagaccagc cactggcgtc ggcaaattgt ccgcatcagt
    13621 ctcgcattgc tgcctatcca tttctggtct gtggtggcag aactttcccc gctgcatctg
    13681 atggatgatg tgctggggca ggcgatgatt ttcttcaact tgctgctgat tgccttcctg
    13741 gtgtggccga tgtgccgcga aagctggcgt gataaagagt cgcacaccat gcgactggtc
    13801 accattaccg tactgtcgat tatcccgatt gcgttgatgg tgctgaccgc cacgggttat
    13861 ttctacacca cgctgcgcct ctccgggcgt tggattgaaa ccgtttattt ggtgatcatc
    13921 tggaacctgc tgtaccagac ggtattgcgc ggcttaagcg tagcggcgcg gcgtatcgcc
    13981 tggcgtcgtg cgctggcgcg tcggcagaat ctggtgaaag agggcgcaga aggtgccgaa
    14041 ccgccggaag aacccaccat tgcactggag caagttaacc agcagacgct gcgtattacc
    14101 atgttgctga tgtttgcgct gttcggtgtc atgttctggg caatttggtc cgatttgatc
    14161 accgtgttca gctatctcga cagcattacg ctctggcatt acaacggtac tgaagctggt
    14221 gctgcggtgg tgaaaaacgt caccatgggc agtctgttgt ttgcgattat cgcctcaatg
    14281 gtggcctggg cgttgattcg caacctgcct ggtttgctgg aagtgctggt gctctcgcga
    14341 ttgaatatgc gccagggcgc gtcgtatgcc attactacca tccttaacta catcattatt
    14401 gccgttggtg cgatgacggt gttcggatcg ctgggcgtct cttgggataa actccagtgg
    14461 ctggcggcag cattatccgt aggtcttggt tttggtttac aagaaatttt cggtaacttc
    14521 gtctccggtt tgatcattct gttcgaacgt ccggtgcgca ttggcgatac ggtaaccatt
    14581 ggtagcttct cggggacggt aagtaagatc cgtattcgtg cgacaacgat taccgatttc
    14641 gatcgcaaag aagtgatcat cccgaacaaa gcgtttgtta ccgagcgtct gatcaactgg
    14701 tcgttgactg acacgactac gcgtctggtg atccgtctcg gcgtggccta tggctccgat
    14761 ctggaaaaag tgcgtaaagt attactgaag gcggcgactg agcacccaag ggtgatgcac
    14821 gaaccaatgc cggaagtctt ctttacggcg tttggtgcca gcacgttgga tcatgagctg
    14881 cgtctgtatg tgcgtgaact gcgtgaccgt agccgtactg tcgatgagct gaaccgtact
    14941 atcgatcagc tgtgccgtga aaacgacatc aacattgcct ttaaccagct tgaagtgcat
    15001 ctgcacaacg agaagggcga tgaggtgacg gaagtcaaac gcgactacaa aggcgacgat
    15061 ccgacaccag cggtagggta ataaccaaag ggggcgacat ttagttgccc cgagattact
    15121 gacaaagtgc gcgttgttca tgccggatgc ggcgtgaccg ccttatccgg cctacgaaac
    15181 cgcaagaatt caatatattg caggagcggt gtaggcctga taagcgtagc gcatcaggca
    15241 gttttgcgtt tgcccgcaat cttaggggac atttagcgac cccatttatt tctcactttt
    15301 ccgcctcatc atcgctctgt aacttctttt cataatcacg ctttacaata tccagcgcgc
    15361 gcagcacggt gcgggctggg atctgatttt cttccagcag tacaatcaaa tcgacagcca
    15421 gtttgacatc gtcaggggca ttttccagtg acatactttc tccattgcta gcgggttaaa
    15481 cgcgctagcc tgttttcgat tttatccagc gcatggcggc agcgtgccag gcgcgcttca
    15541 taggcttcca cttcacgatg cagcgtttgc tgttccacga gatcggtcac ccgcgccagg
    15601 cgggctcgac gttcggcaac catctcacgt agccgccgct caaaatcctg atgctgaata
    15661 cgtttacgct gccagcgggc aattttcggt ggcgcactgt cccactcgcg tagcgaccag
    15721 gctgtggctt cacgcgctat agcctccagt tgcgccgcca gatgttccgc cagccaggcc
    15781 acttgcggca gttgctgttg ctcaacggca tgacgaagcg cagacagatt atcgcccgcc
    15841 tcatcgagac aagcctgtag tgttgtcgca cgggtctgaa aaaggtgcct gtcaaaacgg
    15901 gcacttagcg tggcaaattg cgctaccggg gcacaacgct gacgcagcgt ggcgagctgt
    15961 ccttccagtt tttccagcag cagggcggtt ttcacgataa gacctcaatg aaaatgataa
    16021 ttgttatgct aaagtagcaa ctctgtaagc tgacaacact caatatgcaa cgaatcattt
    16081 taatcatcat tggctggctg gcggtagtgc tgggtacgct gggcgtggta ttaccggtat
    16141 taccgacgac gccgtttatc ctgctggcgg cctggtgctt tgcccgttct tccccgcgct
    16201 ttcacgcctg gttgctgtac cgctcatggt ttggcagcta tctacgtttc tggcagaaac
    16261 atcatgcgat gccgcgcggc gtcaaaccgc gggcgatttt gcttattttg ctcacgtttg
    16321 ccatttctct gtggttcgtc cagatgccat gggtgcgcat catgttgctg gtaattctcg
    16381 cctgtttgct tttttatatg tggcgaattc cggtgattga tgaaaagcaa gaaaagcact
    16441 gaagcacaac aatcgcagtt gcaattattg cgtacagcca gtacattctg gcgttttcga
    16501 gcacaggcgc aggcggtcaa aggttaaaca actgttactt ttgatacgtt taaaacgcgc
    16561 cgtgagtacc accgtaacaa gcaggcatac acttatgacc gcgactgcac agcagcttga
    16621 gtatctcaaa aatagcatca aaagcattca ggactaccca aaacccggca ttcttttccg
    16681 tgatgtcacc agcttactgg aagacccgaa agcttacgct ctcagcatcg acttgctggt
    16741 tgagcgttac aaaaatgcgg gcattaccaa agttgtcggc accgaagcgc gtggcttctt
    16801 gtttggcgct ccggtagctc tgggtctggg cgttggcttt gtaccggtcc gtaaaccggg
    16861 caaactgccg cgtgaaacca tcagtgaaac ttacgacctg gaatacggca ccgatcagct
    16921 ggaaatccac gttgatgcca tcaaaccggg cgacaaagtt ctggtggtgg acgatctgct
    16981 ggcaaccggc ggcactatcg aagcgaccgt taaactgatc cgtcgtctgg gtggtgaagt
    17041 agctgacgct gcgttcatta tcaacctgtt cgatctcggc ggcgagcagc gtctcgaaaa
    17101 acagggcatt accagctaca gccttgtccc gttcccgggc cattaattat cgccagtctt
    17161 gtgctgccca cgctacggac agcacaagat gtgcattcag cctcgccgtt ctgacggggc
    17221 tgtgttagca ttaccccctc gtgaatccac cttccagcgt ttcagagcct gccaatgagt
    17281 tatcaggtct tagcccgaaa atggcgccca caaacctttg ctgacgtcgt cggccaggaa
    17341 catgtgctga ccgcactggc gaacggcttg tcgttagggc gtattcatca tgcttatctt
    17401 ttttccggta cccgtggcgt cggaaaaacc tctatcgccc gactgctggc gaaggggcta
    17461 aactgcgaaa ccggcattac cgcgacgccg tgcggcgtgt gcgataactg tcgtgaaatc
    17521 gagcaggggc gctttgtcga tctgattgaa atcgacgccg cctcgcgtac taaagttgaa
    17581 gacactcgcg acctgctgga taacgtccag tacgctccgg cgcgtggtcg tttcaaagtt
    17641 tatctcatcg acgaagtgca tatgctgtcg cgccacagct ttaacgcact gttaaaaacc
    17701 cttgaagagc cgccggagca cgttaagttt ctgctggcga cgaccgatcc gcagaaattg
    17761 ccggtgacga ttttgtcgcg ctgtctgcaa ttccatctca aggcgctgga tgtcgagcaa
    17821 attcgccatc agcttgagca catcctcaac gaagaacata tcgctcacga gccgcgggcg
    17881 ttgcaattac tggcgcgcgc cgctgaaggc agcttgcgag atgccttaag tctgaccgac
    17941 caggcgattg ccagcggtga cggccaggtt tcaacccagg ccgtcagtgc gatgctgggt
    18001 acgcttgacg acgatcaggc gttgtcgctg gttgaagcga tggtcgaggc caacggcgag
    18061 cgcgtaatgg cgctaattaa tgaagctgct gcccgtggta tcgagtggga agcgttgctg
    18121 gtggaaatgc tcggtttgtt gcatcgtatt gcgatggtac aactttcgcc tgctgcactt
    18181 ggcaacgaca tggccgccat cgagctgcgg atgcgtgaac tggcgcgtac cataccgccg
    18241 acggatattc agctttacta tcaaacgctg ttgattggtc gcaaagaatt accgtatgcg
    18301 ccggaccgcc gcatgggcgt tgagatgacg ctgctgcgcg cgctggcgtt ccatccgcgt
    18361 atgcctctgc ctgagccaga agtgccacgc cagtcctttg cgcccgttgc gccaacggca
    18421 gtaatgacgc caacccaggt gccgccgcaa ccgcaatcag cgccgcagca ggcaccgact
    18481 gtaccgctcc cggaaaccac cagccaggtg ctggcggcgc gccagcagtt gcagcgcgtg
    18541 cagggagcaa ccaaagcaaa aaagagtgaa ccggcagccg ctacccgcgc gcggccggtg
    18601 aataacgctg cgctggaaag actggcttcg gtcaccgatc gcgttcaggc gcgcccggtg
    18661 ccatcggcgc tggaaaaagc gccagccaaa aaagaagcgt atcgctggaa ggcgaccact
    18721 ccagtgatgc agcaaaaaga agtggtcgcc acgccgaagg cgctgaaaaa agcgctggaa
    18781 catgaaaaaa cgccggaact ggcggcgaag ctggcggcag aagccattga gcgcgacccg
    18841 tgggcggctc aggtgagtca actttcgcta ccaaaactgg tcgaacaggt ggcgttaaat
    18901 gcctggaaag aggagagcga caacgcagta tgtctgcatt tgcgctcctc tcagcggcat
    18961 ttgaacaacc gcggcgcaca gcaaaaactg gctgaagcgt tgagcacgtt aaaaggttca
    19021 acggttgaac tgactatcgt tgaagatgat aatcccgcgg tgcgtacgcc gctggaatgg
    19081 cgtcaggcga tatacgaaga aaaacttgcg caggcgcgcg agtccattat tgcggataat
    19141 aatattcaga ccctgcgtcg attcttcgat gcggagctgg atgaagaaag tatccgcccc
    19201 atttgatcgt aagcacagct tacgttcgtc atccttaacg tgattgagag agaaacctat
    19261 gtttggtaaa ggcggtctgg gtaacctgat gaagcaagcc cagcagatgc aagaaaaaat
    19321 gcagaaaatg caggaagaga tcgcgcagct ggaagtcacc ggcgaatctg gcgcaggtct
    19381 ggtaaaagtg accatcaacg gtgcacacaa ctgccgtcgc gtagagatcg acccgagcct
    19441 gctggaagac gacaaagaga tgctggagga cctggtggct gcagcattca acgacgcagc
    19501 acgtcgtatt gaagaaacgc agaaagaaaa aatggcctct gtatcctccg gaatgcagct
    19561 gccgcctggc tttaagatgc cgttctgatg caaaccagcc cgctgttaac acagcttatg
    19621 gaagcactgc gctgtctgcc gggcgttggc ccgaagtcgg cgcagcgtat ggcgttcacg
    19681 ctgcttcagc gcgatcgtag cggcgggatg cgtctggcgc aggcgctcac ccgggcgatg
    19741 tcggaaatcg gccactgcgc cgattgccgc accttcaccg aacaggaagt ctgtaacatc
    19801 tgttcgaatc cgcgtcgtca ggaaaacggt caaatctgcg tggtggagag tccggcggac
    19861 atttacgcca ttgagcagac ggggcagttt tcaggtcgtt attttgtgtt gatggggcat
    19921 ctgtcaccgc tggacggcat cggtccggat gatatcggcc ttgatcgtct ggaacagcgt
    19981 ctggcagagg aaaaaatcac tgaagtgatc ctcgccacca atcccacggt tgaaggtgaa
    20041 gctaccgcta actacattgc cgagctttgt gcgcaatatg acgtggaagc cagccgaatc
    20101 gctcatggcg taccggttgg cggcgaactg gaaatggtcg atggcaccac gctgtcacac
    20161 tcccttgccg ggcgtcataa gattcgtttt taagcaaacg agagcaggat cacctgctct
    20221 cgcttgaaat tattctccct tgtccccatc tctccctcat cctgttttta accttaaaat
    20281 ggcattattg aggtagacct acatgaaagg acaagaaact cgtggttttc agtcagaagt
    20341 gaaacagctt ctgcacctga tgatccattc tctctattcc aataaagaaa tcttcctgcg
    20401 tgagcttatc tctaacgcct ccgatgcggc ggacaagctg cgtttccgcg cgctctctaa
    20461 cccggacctg tacgaaggtg atggcgaact gcgcgttcgt gtctctttcg ataaagacaa
    20521 gcgcacgctg accatctccg ataacggcgt ggggatgacc cgcgacgaag tgattgacca
    20581 tctggggact atcgctaaat ccggcaccaa atcattcctc gaatccctgg gttctgacca
    20641 ggcgaaagac agccagctga tcggtcagtt tggtgttggt ttctactctg cgtttatcgt
    20701 ggccgacaaa gtgaccgtgc gtactcgcgc ggcaggcgaa aaaccagaaa atggcgtctt
    20761 ctgggaatcg gctggcgaag gtgaatacac cgttgccgat atcaccaaag aagatcgtgg
    20821 tactgaaatc accctgcatc tgcgtgaagg cgaagacgag ttcctcgatg actggcgcgt
    20881 gcgttccatc atcagcaaat actccgacca tatcgcgctg ccggtagaga tcgaaaaacg
    20941 cgaagagaaa gacggcgaaa ccgttatctc ctgggagaaa atcaacaaag cgcaggcgct
    21001 gtggactcgt aacaagtcgg aaatcaccga tgaagagtac aaagagttct acaaacacat
    21061 cgcccacgac tttaacgatc cgctgacctg gagccacaac cgcgttgaag gtaagcagga
    21121 gtacaccagc ctgctgtaca ttccgtccca ggctccgtgg gatatgtgga accgcgatca
    21181 taaacacgga ctgaaactgt acgtccagcg tgtgttcatc atggacgacg cagaacagtt
    21241 catgccgaac tatctgcgct tcgtgcgtgg tctgattgac tccagcgatc tgccgctgaa
    21301 cgtttcccgt gaaatcctcc aggacagcac ggtaacgcgt aacctgcgca atgcgctgac
    21361 caagcgtgtg ctgcaaatgc tggaaaaact ggcgaaagac gacgcggaaa aataccagac
    21421 cttctggcaa cagtttggcc tggtactgaa agaaggtccg gcggaagatt tcgctaacca
    21481 ggaagcgatc gccaaattgc tgcgttttgc ttctacccat accgattctt ctgcgcagac
    21541 cgtatctctg gaagactacg tttcccgcat gaaagaaggg caggagaaaa tctactacat
    21601 caccgcagac agctatgcgg cagcgaagag cagcccgcac ctggaactgc tgcgtaagaa
    21661 aggcatcgaa gttctgctgc tttccgaccg catcgatgag tggatgatga actatctgac
    21721 tgagttcgac ggtaaaccgt tccagtctgt gtctaaagtt gacgagtcgc tggaaaaact
    21781 ggctgacgaa gttgatgaga gcgcgaaaga agcggagaaa gcactgactc cgttcatcga
    21841 ccgtgtgaaa gccctgctcg gcgagcgcgt gaaagatgtc cgtctgactc accgtctgac
    21901 cgatacgcca gcgattgtct ctaccgacgc ggacgaaatg agcacccaga tggcgaaact
    21961 gtttgccgca gcgggccaga aagtgccgga agtgaaatac atcttcgaac tgaacccgga
    22021 tcacgtactg gtgaaacgtg cggcagatac tgaagatgaa gccaagttca gcgagtgggt
    22081 agaactgctg ctggatcagg cgctgctggc agaacgcggc acgctggaag atccgaacct
    22141 gtttattcgt cgtatgaacc agctgctggt ttcctgatgt catgccggat gacctttgtg
    22201 tcatccggca tttttctttt catcatctgc actttccgca aattatctcg ccattaaccg
    22261 tttcagcccc aggtgccttt cttgaggcaa tcgcttgttg gtggtatcgt ttatcgcttt
    22321 ttcaaaaaat tcgacacatt ttaaggggat tttcgcaatg cgtatcattc tgcttggcgc
    22381 tccgggcgcg gggaaaggga ctcaggctca gttcatcatg gagaaatatg gtattccgca
    22441 aatctccact ggcgatatgc tgcgtgctgc ggtcaaatct ggctccgagc tgggtaaaca
    22501 agcaaaagac attatggatg ctggcaaact ggtcaccgac gaactggtga tcgcgctggt
    22561 taaagagcgc attgctcagg aagactgccg taatggtttc ctgttggacg gcttcccgcg
    22621 taccattccg caggcagacg cgatgaaaga agcgggcatc aatgttgatt acgttctgga
    22681 attcgacgta ccggacgaac tgattgttga tcgtattgta ggccgccgcg ttcatgcgcc
    22741 gtctggtcgt gtttatcacg ttaaattcaa tccgcctaaa gtagaaggca aagacgacgt
    22801 taccggtgaa gaactgacta cccgtaaaga cgatcaggaa gaaaccgtgc gtaaacgtct
    22861 ggttgaatac catcagatga ctgcaccgct gatcggctac tactccaaag aagcggaagc
    22921 aggtaacacc aaatacgcga aagttgacgg caccaagccg gttgctgaag ttcgcgctga
    22981 tctggaaaaa atcctcggct aatcaagccg cagcagatct gctttcgggc aggtctgctc
    23041 atcctcctca ggcaatacct ctcacagcaa ttagttcttc ttcttcactt ttccgctaca
    23101 attatcaaca agttgaatcg ataagaggcg gtaatgcgtc agactaaaac cggtatcctg
    23161 ctggcaaacc tgggtacgcc cgatgccccc acacctgaag cggtaaaacg ctatctgaaa
    23221 caatttttaa gcgacagacg cgtggttgat acctcacggt tgttatggtg gccgttgctg
    23281 cgcggcgtga ttttgccgct gcgctcgccg cgtgtggcga agctgtatgc ctctgtctgg
    23341 atggaaggtg gctcgccgct gatggtttac agccgccagc aacagcaggc gctggcacaa
    23401 cgtttaccgg agacgcccgt agcgctggga atgagctacg gctcgccatc actggaaagc
    23461 gccgtagatg aacttctggc agagcatgta gatcatattg tggtgctgcc gctttatccg
    23521 caatactcct gttcaactgt cggtgcggta tgggatgaac tggcgcgaat tctggcgcgc
    23581 aaacgtagca ttccggggat atcgtttatt cgtgattacg ccgataacca cgattatatt
    23641 aatgcactgg cgaacagcgt acgcgcttct tttgccaaac atggcgaacc ggatctgctg
    23701 ttgctctctt atcatggcat tccccagcgt tatgcagatg aaggcgatga ttatccgcaa
    23761 cattgccgca caacgactcg cgaactggct tccgcactgg agatggcacc ggaaaaagtg
    23821 atgatgacct ttcagtcgcg ctttgggcgg gaaccctggc tgatgcctta taccgacgaa
    23881 acgctgaaaa tgctcggaga aaaaggcgta ggtcatattc aggtgatgtg cccgggcttt
    23941 gctgcggatt gtctggagac gctggaagag attgccgagc aaaaccgtga ggtcttcctc
    24001 ggtgcgggcg ggaaaaaata tgaatatatt ccggcgctta atgccacgcc agaacatatc
    24061 gaaatgatgg ctaatcttgt tgccgcgtat cgctaaagct gagcggtaaa gaactgagcg
    24121 ccgtcgcgaa gagcctcgtc ggcggttttc atcatccgtg aataatgcaa aaaggcgtgc
    24181 agcgtgcctg ggtagagttt gaactcgcag ggctgctgat gcgccgctaa cgtctggtaa
    24241 agcagacggc tgtcatccag cagcggatcg aactccgccc cggcaataaa acagggcggg
    24301 acttcgcgag tgagatcatt attaaacagg cagtaatacg gcgactcgcg gtccgcgtcg
    24361 ttgcttaaat acgcctcttc atacatctgt aaatcctgtt gcgttaagcc atcccagaca
    24421 ccgcccaaca gacgacgagt cacggaatcc cgtaatccgt aaagcccata ccacagcaaa
    24481 acgcccgcaa ctttaccgca atcgatctgt ttatcacgca accacaacgc actggcgagc
    24541 gccagcatgg cacctgcgga atcaccggca aagccaatgc gggacatatt gatttgataa
    24601 tcctccgcct gctggtggaa ataacaacag gcagccacaa tttcctctat cgcttgcgga
    24661 aaacgcgctt caggtgaaag ggtgtaatca atgccaatca ccgtacattg gctgtagctt
    24721 gccagcaggc gcatgatgcg atcgtgggta tcgagattgc cgagaataaa accgccgcca
    24781 tgcaaataaa atagcgtcgc cggactatct ggctgcggac aaaagagacg tgtttccacc
    24841 tgcccatatt ttgttggaac catgtaagct ctggttgcca tttctggagc gcccgcattc
    24901 cagaatcggc gctcaagcgt gtaatactgt cgttgctcag caatcgttcc cgttgcgggc
    24961 cagggcggta aatccggctg aagagtattc acaacggtct tcatttcagc agaaataagg
    25021 tccagaacgg gtaatttgtt ttccggcttc atgcaaaaac tcctttcaaa ttacgtcatt
    25081 gtaaggaacc actgccatga aaatgcgatc ccgcctgctg atattgaaac tggctgcatc
    25141 tcgcgcgctc ccgtcagatt gtgttaacat tcgccgctca gttaaccacc cgtaaaaaca
    25201 accatgaaat ttcccggtaa acgtaaatcc aaacattact tccccgtaaa tgcacgcgat
    25261 ccgctgcttc agcaattcca gccagaaaac gaaaccagcg ctgcctgggt agtgggtatc
    25321 gatcaaacgc tggtcgatat tgaagcgaaa gtggatgatg aattcattga gcgttatgga
    25381 ttaagcgccg ggcattcact ggtgattgag gatgacgtag ccgaagcgct ttatcaggaa
    25441 ctaaaacaga aaaacctgat tacccatcag tttgcgggtg gcaccattgg caacaccatg
    25501 cacaactact cggtgctcgc ggacgaccgt tcggtgctgc tgggtgtgat gtgcagcaat
    25561 attgaaattg gcagctatgc ctatcgttac ctgtgtaata cctccagccg taccgatctt
    25621 aactatctac aaggcgtgga tggcccgatt ggtcgttgct ttacgctgat tggcgagtcc
    25681 ggggaacgta cctttgctat cagccccggc cacatgaacc agctgcgggc tgaaagtatt
    25741 ccggaagatg tgattgccgg agcctcggct ctggttctca cctcttatct ggtgcgttgc
    25801 aagccgggtg aacccatgcc ggaagcaact atgaaagcca ttgagtacgc gaagaaatat
    25861 aacgtaccgg tggtgctgac gctgggcacc aagtttgtca ttgccgagaa tccgcagtgg
    25921 tggcagcaat tcctcaaaga ccacgtctct atccttgcga tgaacgaaga tgaagccgaa
    25981 gcgttgaccg gagaaagcga tccgttgttg gcatctgaca aggcgctgga ctgggttgat
    26041 ctggtgctgt gcaccgccgg gccaatcggc ttgtatatgg cgggctttac cgaagacgaa
    26101 gcgaaacgta aaacccagca tccgctgctg ccgggtgcta tagcggaatt taaccagtat
    26161 gagtttagcc gcgccatgcg ccacaaggat tgccagaatc cgctgcgtgt ctattcgcac
    26221 attgcgccgt acatgggcgg gccggaaaaa atcatgaata ccaacggagc aggagatggc
    26281 gcactggcag cgttgctgca tgacattacc gccaacagct accaccgtag caacgtacca
    26341 aactccagca aacataaatt cacctggtta acttattcat cgttagcgca ggtgtgtaaa
    26401 tatgctaacc gtgtgagcta tcaggtactg aaccagcatt cacctcgttt aacgcgcggc
    26461 ttgccgga
//
LOCUS       DAMZFT010000062        26426 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.062, whole genome shotgun sequence.
ACCESSION   DAMZFT010000062 DAMZFT010000000
VERSION     DAMZFT010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 26426)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26426)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26426
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.062"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(<1..432)
                     /gene="casB"
                     /locus_tag="PN977_002683"
     CDS             complement(<1..432)
                     /gene="casB"
                     /locus_tag="PN977_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cse2/CasB"
                     /protein_id="HDI6042701.1"
                     /translation="MSIVKEEHKATLRKWHEELQEKRGNRASLRRSTTVNDVCLSEGF
                     RSLLMQTHTLWKIESQEWRFTALALVAAVAANVKAIDERQPFAAQLAAVMSEGRFTRL
                     SAIKTPDDLLRQLRRAVKLLNGSVNLISLADDIFRWCQESDD"
     gene            complement(429..1991)
                     /gene="casA"
                     /locus_tag="PN977_002684"
     CDS             complement(429..1991)
                     /gene="casA"
                     /locus_tag="PN977_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cse1/CasA"
                     /protein_id="HDI6042702.1"
                     /translation="MNSFSLLTTPWLPVRFKDGTTGKLAPVDLADENVVDIAAPRADL
                     QGAAWQFLLGLLQTSFAPKDHRRWDDIWEDGLEAEKLREALLSLEHAFQFGPDSSSFM
                     QDFEALTGDKVPVASLLPEIPGSQTTKFNKDHFIKRGVTEYLCPHCLALALFSLQLNA
                     PAGGKGYRTGLRGGGPMTTLIELQEYQGNQQTPLWRKLWLNVMPQDEADLPLPKKFDD
                     LIFPWLGPTRTSELVGAVVTHDQVNKLQAYWGMPRRIRIDFNTTTVGNCDICDEQNDA
                     LLSLMTTKNYGANYAMWQHPLTPYRVPLKEGGEFYSVKPQPGGLIWRDWLGLIETGKS
                     ENNTELPALVVKLFNASSLKQAKVGLWGFGYDFDNMKARCWYEHHFPLLLNKKEGQIP
                     KLRLAAQTASRILSLLRSALKEAWFSDPKGARGDFSFVDIDFWNKTQHRFLRLVRQIE
                     EGQDADELLGKWQKEMWLFARQDFDERVFTNPYEPVDLKRVMTARKKYFTTSAEKQSA
                     KAAREKKQEAAE"
     gene            complement(2089..4788)
                     /gene="cas3"
                     /locus_tag="PN977_002685"
     CDS             complement(2089..4788)
                     /gene="cas3"
                     /locus_tag="PN977_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001233973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated helicase Cas3'"
                     /protein_id="HDI6042703.1"
                     /translation="MRKYPLSLLKDKNIVTFFDFWGKTRRGEKDGGDDYHLLCWHSLD
                     VAAMGYLMVKSNCFGLADYFRQLGFADTEQAAQFFAWLLCWHDIGKFARSFQQLYLHP
                     QLKVPEGARKNYEKISHSTLGYWLWNHYLSECQELLPASSLSPRKLKRVMEMWMPMTT
                     GHHGRPPERMDELDNFLPEDKGAARDFLLAIKVLFPLIEIPTFWDDDEGVELIKQLSW
                     YISATVVLADWTGSSTRYFPRVAQTMDIEDYWQKTLVQAQNALTVFPPKAESAPFTGI
                     NTLFPFIENPTPLQQKVLDLDISQPGPQLFILEDVTGAGKTEAALILAHRLMAAGKAQ
                     GLFFGLPTMATANAMYDRLVKTWLAFYSPESRPSLVLAHSARTLMDRFNESLWSGDLV
                     GSEEPDEQTFSQGCAAWFADSNKKALLAEIGVGTLDQAMMAVMPFKHNNLRLLGLSNK
                     ILLADEIHACDAYMSCILEGLIERQARGGNSVILLSATLSQQQRDKLVAAFARGIEGQ
                     QEAPFLEKDDYPWLTHVTKSDVHSHRVATRKDVERSVSVGWLHSEQECIARIASAVSQ
                     GKCIAWIRNSVDDAIQVHRQLLARGVIPASSLSLFHSRFAFSDRQRIETETLARFGKE
                     DCSQRAGKVLICTQVLEQSVDCDLDEMISDLAPVDLLIQRAGRLQRHIRDMNGQLKRD
                     GKDERHPPELLILAPVWDDSPGDEWFGSAMRNSAFVYPDHGRIWLTQRVLREQGAIQM
                     PHSARLLIESVYGEDVVMPEGFARSEQEQVGKYYCDRAMAKKFVLNFRPGYAANINDY
                     LPEKLSTRLAEESVSLWLATCIDGVVKPYATGAHAWEMSVVRVRRSWWKKHRDEFSLL
                     EGDAFRQWCVEQRHDPEMANVILVTDDESCGYSATEGLIGKVG"
     gene            complement(4983..5135)
                     /locus_tag="PN977_002686"
     CDS             complement(4983..5135)
                     /locus_tag="PN977_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Hok family toxin"
                     /protein_id="HDI6042704.1"
                     /translation="MLTKYALVAIIVLCFTVLGFTLMVGDSLCELSIRERGMEFKAVL
                     AYESKK"
     gene            complement(5400..6134)
                     /gene="cysH"
                     /locus_tag="PN977_002687"
     CDS             complement(5400..6134)
                     /gene="cysH"
                     /locus_tag="PN977_002687"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HDI6042705.1"
                     /translation="MSKLDLNALNELPKVDRILALAETNAELEKLDAEGRVAWALDNL
                     PGEYVLSSSFGIQAAVSLHLVNQIHPDIPVILTDTGYLFPETYRFIDELTDKLKLNLK
                     VYRATESAAWQEARYGKLWEQGVEGIEKYNDINKVEPMNRALKELNAQTWFAGLRREQ
                     SGSRANLPVLAIQRGVFKVLPIIDWDNRTIYQYLQKHGLKYHPLWDEGYLSVGDTHTT
                     RKWEPGMAEEETRFFGLKRECGLHEG"
     gene            complement(6208..7920)
                     /gene="cysI"
                     /locus_tag="PN977_002688"
     CDS             complement(6208..7920)
                     /gene="cysI"
                     /locus_tag="PN977_002688"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001290736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="assimilatory sulfite reductase (NADPH)
                     hemoprotein subunit"
                     /protein_id="HDI6042706.1"
                     /translation="MSEKHPGPLVVEGKLTDAERMKLESNYLRGTIAEDLNDGLTGGF
                     KGDNFLLIRFHGMYQQDDRDIRAERAEQKLEPRHAMLLRCRLPGGVITTKQWQAIDKF
                     AGENTIYGSIRLTNRQTFQFHGILKKNVKPVHQMLHSVGLDALATANDMNRNVLCTSN
                     PYESQLHAEAYEWAKKISEHLLPRTRAYAEIWLDQEKVATTDEEPILGQTYLPRKFKT
                     TVVIPPQNDIDLHANDMNFVAIAENGKLVGFNLLVGGGLSIEHGNKKTYARTASEFGY
                     LPLEHTLAVAEAVVTTQRDWGNRTDRKNAKTKYTLERVGVETFKAEVERRAGIKFEPI
                     RPYEFTGRGDRIGWVKGIDDNWHLTLFIENGRILDYPGRPLKTGLLEIAKIHKGDFRI
                     TANQNLIIAGVPESEKAKIEKIAKESGLMNAVTPQRENSMACVSFPTCPLAMAEAERF
                     LPSFIDKIDNLMAKHGVSDEHIVMRVTGCPNGCGRAMLAEVGLVGKAPGRYNLHLGGN
                     RIGTRIPRMYKENITEPEILASLDELIGRWAKEREAGEGFGDFTVRAGIIRPVLDPAR
                     DLWD"
     gene            complement(7920..9719)
                     /gene="cysJ"
                     /locus_tag="PN977_002689"
     CDS             complement(7920..9719)
                     /gene="cysJ"
                     /locus_tag="PN977_002689"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent assimilatory sulfite reductase
                     flavoprotein subunit"
                     /protein_id="HDI6042707.1"
                     /translation="MTTQVSPSALLPLNPEQLARLQAATTDLTPTQLAWVSGYFWGVL
                     NQQPAALAATPAPAAEMPGITIISASQTGNARRVAEALRDDLLAAKLNVKLVNAGDYK
                     FKQIASEKLLIVVTSTQGEGEPPEEAVALHKFLFSKKAPKLENTAFAVFSLGDSSYEF
                     FCQSGKDFDSKLAELGGERLLDRVDADVEYQAAASEWRARVVDALKSRAPVAAPSQSV
                     ATGTVNEIHTSPYSKDAPLVASLSVNQKITGRNSEKDVRHIEIDLGDSGLRYQPGDAL
                     GVWYQNDPALVKELVELLWLKGDEPVTVEGKTLPLNEALQWHFELTVNTANIVENYAT
                     LTRSETLLPLVGDKAKLQHYAATTPIVDMVRFSPAQLDAEALINLLRPLTPRLYSIAS
                     SQAEVENEVHVTVGVVRYDVEGRARAGGASSFLADRVEEEGEVRVFIEHNDNFRLPAN
                     PETPVIMIGPGTGIAPFRAFMQQRAADEAPGKNWLFFGNPHFTEDFLYQVEWQRYVKE
                     GVLTRIDLAWSRDQKEKIYVQDKLREQGAELWRWINDGAHIYVCGDANRMAKDVEQAL
                     LEVIAEFGGMDTEAADEFLSELRVERRYQRDVY"
     gene            10038..10400
                     /gene="queD"
                     /locus_tag="PN977_002690"
     CDS             10038..10400
                     /gene="queD"
                     /locus_tag="PN977_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708563.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HDI6042708.1"
                     /translation="MSTTLFKDFTFEAAHRLPHVPEGHKCGRLHGHSFMVRLEITGEV
                     DPHTGWIIDFAELKAAFKPTYERLDHHYLNDIPGLENPTSEVLAKWIWDQVKPVVPLL
                     SAVMVKETCTAGCIYRGE"
     gene            10478..11749
                     /locus_tag="PN977_002691"
     CDS             10478..11749
                     /locus_tag="PN977_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417246.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HDI6042709.1"
                     /translation="MEDDCDIIIIGAGIAGTACALRCARAGLSVLLLERAEIPGSKNL
                     SGGRLYTQALAELLPQFHLTAPLERRITHESLSLLTPDGATTFSSLQPGGESWSVLRA
                     RFDPWLVAEAEKEGVECIPGATVDALYEENGRVCGVICGDDILRARYVVLAEGANSVL
                     AERHGLVTRPAGEAMALGIKEVLSLETSAIEERFHLENNEGAALLFSGGICDDLPGGA
                     FLYTNQQTLSLGIVCPLSSLTQSRVPASELLARFKTHPAVRPLIKNTESLEYGAHLVP
                     EGGLHSMQVQYAGNGWLLVGDTLRSCVNTGISVRGMDMALTGAQAAAQTLISACQHRE
                     PQNLFALYHHNVERSLLWDVLQRYQHVPALLQRPGWYRAWPALMQDISRDLWDQGDKP
                     VPPLHQLFWRHLRRHGLWHLAGDVIRSLRCL"
     gene            11740..12000
                     /locus_tag="PN977_002692"
     CDS             11740..12000
                     /locus_tag="PN977_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HDI6042710.1"
                     /translation="MSVARNLWRVADAPHIVPADSVERQTVQWLINACPAGLFSLTPE
                     GDLRIDYRSCLECGTCRLLCDEKTLQQWRYPPSGFGITYRFG"
     gene            12017..12592
                     /locus_tag="PN977_002693"
     CDS             12017..12592
                     /locus_tag="PN977_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate responsive antiterminator"
                     /protein_id="HDI6042711.1"
                     /translation="MPLLHLLRQNPVIAAVKDNASLQLAIDSECQFISVLYGNICTIS
                     NIVKKIKNAGKYAFIHVDLLEGASNKEVVIQFLKLVTEADGIISTKASMLKAARAEGF
                     FCIHRLFIVDSISFHNIDKQVAQSNPDCIEILPGCMPKVLGWVTEKIRQPLIAGGLVC
                     DEEDARNAINAGVVALSTTNTGVWTLAKKLL"
     gene            complement(12739..13599)
                     /locus_tag="PN977_002694"
     CDS             complement(12739..13599)
                     /locus_tag="PN977_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417249.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HDI6042712.1"
                     /translation="MNIAIVTINQENAAIASWLAEQDFSGCTLAHWQIEPQPVVAEQV
                     LDALVEQWQRTPADVVLFPPGTFGDELSTRLAWRLHGASICQVTSLDIPTVSVRKSHW
                     GNALTATLQTDKRPLCLSLARQAGADKNATLSSGMQQLNIVPGALPDWLVSLENLKNV
                     TRDPLAEARRVLVVGQGGEADNQEIAMLAEKLGAEVGYSRARVMNGGVDAEKVIGISG
                     HLLAPEVCIVVGASGAAALMAGVRNSKFVVAINHDASAAVFSQADVGVVDDWKVVLEA
                     LVTNIHADCQ"
     gene            complement(13596..14375)
                     /locus_tag="PN977_002695"
     CDS             complement(13596..14375)
                     /locus_tag="PN977_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HDI6042713.1"
                     /translation="MNILLAFKAEPDAGMLAEKEWQAAAQGNIGPDVSLLRSLLGADE
                     QAAAALLLAQRKNGTPMSLTALSMGDERALHWLRYLMALGFEEAVLLETAADLRFAPE
                     FVARHIAEWQHQNPLDLIITGCQSSEGQNGQTPFLLAEMLGWPCFTQVERFTLDAPFI
                     TLEQRTENGVRCCRVRLPAVIAVRQCGEVALPVPGMRQRMAAGKAEIIRKTVAVELPA
                     MQCLQLARAEQRRGATLIDGQTVAEKAQKLWQDYLRQRMQP"
     gene            complement(14353..15762)
                     /locus_tag="PN977_002696"
     CDS             complement(14353..15762)
                     /locus_tag="PN977_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDI6042714.1"
                     /translation="MTGRCLFGFSGEKPFLLPDNEGVKMNTSPVRMDDLPLNRFHCRI
                     AALTFGAHLTDGYVLGVIGYAIIQLTPAMQLTPFMAGMIGGSALLGLFLGSLVLGWIS
                     DHIGRQKIFTFSFLLITLASFLQFFATTPEHLIGLRILIGIGLGGDYSVGHTLLAEFS
                     PRRHRGILLGAFSVVWTVGYVLASIAGHHFISENPEAWRWLLASAALPALLITLLRWG
                     TPESPRWLLRQGRFAEAHAIVHRYLGPHVLLGDEVAAATHKHIKTLFSSRYWRRTAFN
                     SVFFVCLVIPWFVIYTWLPTIAQTIGLEDALTASLMLNALLIVGALLGLVLTHLLAHR
                     KFLLGSFLLLAATLVVMACLPSGSSLTLLLFVLFSTTISAVSNLVGILPAESFPTDIR
                     SLGVGFATAMSRLGAAVSTGLLPWVLAQWGMQATLLLLAAVLLVGFVVTWLWAPETKA
                     LPLVAAGNVGGANEHSVSV"
     gene            complement(15784..17238)
                     /locus_tag="PN977_002697"
     CDS             complement(15784..17238)
                     /locus_tag="PN977_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HDI6042715.1"
                     /translation="MSLSRAAIVDQLKEIVGADRVITDETVLKKNSIDRFRKFPDIHG
                     IYTLPIPAAVVKLGSTEQVSRVLNFMNAHKINGVPRTGASATEGGLETVVENSVVLDG
                     SAMNQIINVDIENMQATAQCGVPLEVLENALREKGYTTGHSPQSKPLAQMGGLVATRS
                     IGQFSTLYGAIEDMVVGLEAVLADGTVTRIKNVPRRAAGPDIRHIIIGNEGALCYITE
                     VTVKIFKFTPENNLFYGYILEDMKTGFNILREIMVEGYRPSIARLYDVEDGTQHFTHF
                     ADGKCVLIFMAEGNPRIAKATGEGIAEIVARYPQCQRVDSKLIETWFNNLNWGPDKVA
                     AERVQILKTGNMGFTTEVSGCWSCIHEIYESVINRIRTEFPHADDITMLGGHSSHSYQ
                     NGTNMYFVYDYNVVDCKPEEEIDKYHNPLNKIICEETIRLGGSMVHHHGIGKHRVHWS
                     KLEHGSAWTLLEGLKKQFDPNGIMNTGTIYPIEK"
     gene            complement(17308..18093)
                     /locus_tag="PN977_002698"
     CDS             complement(17308..18093)
                     /locus_tag="PN977_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDI6042716.1"
                     /translation="MSIESLNAFSMDFFSLKGKTAIVTGGNSGLGQAFAMALAKAGAN
                     VFIPSFVKDNGETKEMIEKQGVEVDFMQVDITAEGAPQKIIAACCERFGTVDILVNNA
                     GICKLNKVLDFGRADWDPMIDVNLTAAFELSYEAAKIMIPQKSGKIINICSLFSYLGG
                     QWSPAYSATKHALAGFTKAYCDELGQYNIQVNGIAPGYYATDITLATRSNPETNQRVL
                     DHIPANRWGDTQDLMGTAIFLASPASNYVNGHLLVVDGGYLVR"
     gene            18411..19688
                     /locus_tag="PN977_002699"
     CDS             18411..19688
                     /locus_tag="PN977_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDI6042717.1"
                     /translation="MQHNSYRRWITLAIISFSGGVSFDLAYLRYIYQIPMAKFMGFSN
                     TEIGLIMSTFGIAAIILYAPSGVIADKFSHRKMITSAMIITGLLGLLMATYPPLWVML
                     CIQVAFAITTILMLWSVSIKAASLLGDHSEQGKIMGWMEGLRGVGVMSLAVFTMWVFS
                     RFAPDDSASLKTVIIIYSVVYILLGILCWFFVSDNNNLRSANNEEKQSFQLSDILAVL
                     RISTTWYCSMVIFGVFTIYAILSYSTNYLTEMYGMSLVAASYMGIVINKIFRALCGPL
                     GGIITTYSKVKSPTRVIQILSIIGLLALTALLVTNSNPQSVAMGIGLILLLGFTCYAS
                     RGLYWACPGEARTPSYIMGTTVGICSVIGFLPDVFVYPIIGHWQDTLPAAEAYRNMWL
                     MGMAALAMVIIFTFLLFQKIRTADSAPAMASSK"
     gene            19715..21193
                     /locus_tag="PN977_002700"
     CDS             19715..21193
                     /locus_tag="PN977_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HDI6042718.1"
                     /translation="MSKKYIIGIDGGSQSTKVVLYDLEGNVVCEGKGLLQPMHTPDAD
                     TAEHPDDDLWASLCFAGHDLMSQFAGNKEDIVGIGLGSIRCCRALLKADGTPAAPLIS
                     WQDARVTRPYEHTNPDVAYVTSFSGYLTHRLTGEFKDNIANYFGQWPVDYKTWAWSED
                     TAVMEKFNIPRQMLFDVQMPGTVLGHITPQAALATHFPVGLPVVCTTSDKPVEALGAG
                     LLDDETAVISLGTYIALMMNGKALPKDPVAYWPIMSSIPQTLLYEGYGIRKGMWTVSW
                     LRDMLGESLIQDAKAQDLSPEDLLNKKASCVPPGCNGLMTVLDWLTNPWEPYKRGIMI
                     GFDSSMDYAWIYRSILESVALTLKNNYDNMCNEMNHFAKHVIITGGGSNSDLFMQIFA
                     DVFNLPARRNAINGCASLGAAINTAAGLGLYPDYATAVDKMVRVKDIFMPVESNAKRY
                     DAMNKGIFKDLTKHTDVILKKSYEVMHGELGNEDSIQSWSNA"
     gene            21358..22278
                     /locus_tag="PN977_002701"
     CDS             21358..22278
                     /locus_tag="PN977_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001461526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoimidazole riboside kinase"
                     /protein_id="HDI6042719.1"
                     /translation="MSARVWVLGDAVVDLLPESQGRLLQCPGGAPANVAVGIARLGGK
                     SAFIGKVGDDPFGRFMYQTLSTENVDTHYMSLDPQQRTSIVAVGLDEQGERNFTFMVR
                     PSADLFLQPGDLPAFGPGEWLHLCSIALSAEPSRSTAFLAMEKIRQAGGNISFDPNIR
                     SDLWQSEALLRKYLDRALSLANIAKLSEEELLFISGESQVQQGAYSLVQRYSLTLLLI
                     TQGKNGVLVYFQGQFIHYPAKPVSVVDTTGAGDAFVAGLLAGLADSGIPTNTRQLERI
                     IAQAQICGALATTAKGAITALPRQHDLPSQ"
     gene            22446..23963
                     /locus_tag="PN977_002702"
     CDS             22446..23963
                     /locus_tag="PN977_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003857084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate porin"
                     /protein_id="HDI6042720.1"
                     /translation="MYKKTTLAMLIALLTGATTVHAQTDISSIESRLAALEQRLKNAE
                     SRAQAAEARAKTAELQVQKLAETQQQNQLTTQEVAQRTVQLEQKSAENSGFEFHGYAR
                     SGLLMNDAASSSKSGPYLTPAGETGGAVGRLGNEADTYVELNVEHKQTLDNGATTRFK
                     AMLADGQRDYNDWTGGSSNLNIRQAFAELGALPSFTGAFKDSTVWAGKRFDRDNFDIH
                     WLDSDVVFLAGTGGGIYDVKWNDTFRSNFSLYGRNFGDLDDIDNNVQNYILTMNHYAG
                     PFQLMVSGLRAKDNDDRKDANGDLIQTDAANTGVHALVGLHNDTFYGLREGTAKTALL
                     YGHGLGAEVKGIGSDGALLSEANTWRFASYGTTPLGSGWYVAPAILAQSSKDRYVKGD
                     SYEWVTFNTRLIKEVTQNFALAFEGSYQYMDLKPKGYQNHNAVNGSFYKLTFAPTLKA
                     NDINNFFSRPELRLFATWMDWSSKLDDFASNDAFGSSGFNTGGEWNFGVQMETWF"
     gene            24024..25394
                     /locus_tag="PN977_002703"
     CDS             24024..25394
                     /locus_tag="PN977_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sucrose transporter subunit IIBC"
                     /protein_id="HDI6042721.1"
                     /translation="MDFDKIAQSLLPLLGGKENIASAAHCATRLRLVLVDDTLADQHA
                     IGQIDGVKGCFRNSGQMQIIFGTGVVNKVYAAFIQVAGISESSKADTARLAAQKLNPF
                     QRIARLLSNIFVPIIPAIVASGLLMGLLGMVKTYGWVNADNAIYILLDMCSSAAFIIL
                     PILIGFTAAREFGGNPYLGATLGGILTHPALTNAWGVAAGFQTMNFFGFEIAMIGYQG
                     TVFPVLLAVWFMSIVEKQLRRFIPDALDLILTPFLTVVISGFIALLIIGPAGRALGDG
                     ISFVLSTLIAHAGWLAGLLFGGLYSAIVITGIHHSFHAIEAGLLGNPAIGVNFLLPIW
                     AMANVAQGGACLAVWFKTKDTKIKAITLPSAFSAMLGITEAAIFGINLRFVKPFIAAL
                     IGGAAGGAWVVSVHVYMTAVGLTAIPGMAIVQPTSLVNYIIGMVIAFAVAFSLSLLLK
                     YKTDEE"
     gene            25394..>26426
                     /locus_tag="PN977_002704"
     CDS             25394..>26426
                     /locus_tag="PN977_002704"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004111110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HDI6042722.1"
                     /translation="MSMPSQMPAILQAVMKGQSKAQADAHYPCWHLAPVTGLMNDPNG
                     FCWSGGRYHLFYQWNPLDCNHKYKCWGHWSSTDLLHWQHEPLALMPDKEYDRNGCYSG
                     SAVNNQGVLTLCYTGNVKFDDGSRTAWQCLATENNQGGFDKLGPVIPLPDGYTGHVRD
                     PKVWKHNSRWYMVLGAQDKEKRGKVLLYSSVDLYTWSFHGEIAGNGLNEIDNAGYMWE
                     CPDLFALDGEYILLCCPQGMAREHERYLNTYPCAWLHGQFDYETGKFTHGAFSELDAG
                     FEFYAPQTMEAPDGRRLLVGWMGVPDGEEMLQPTRKHHWQHQMTCFRELSFQKGKLFQ
                     MPIRELAQLR"
BASE COUNT         6423 a         6783 c         6530 g         6690 t
ORIGIN      
        1 gtcgtcactc tcctggcacc agcggaaaat atcgtctgcc aaagagataa gattgacgga
       61 accgtttaat aatttgaccg cgcgacgtaa ctggcgcagt aaatcatccg gcgttttgat
      121 tgccgacagg cgagtaaaac gcccctcaga cattaccgcc gccaactgcg cggcaaaagg
      181 ctgccgttca tcaatggctt ttacattggc ggcaacggcg gcaaccagcg ccagtgcggt
      241 aaaacgccac tcctgtgatt caattttcca caacgtgtgg gtctgcatca gcagagaacg
      301 aaaaccttct gacagacaaa catcattaac cgttgtgcta cgccgcagac tggcacgatt
      361 gccgcgtttt tcctgcaact cttcgtgcca tttacgcagc gtggctttgt gctcctcttt
      421 tacaatactc attcagcagc ctcctgcttt ttctccctgg cggctttagc actttgcttc
      481 tccgccgatg ttgtaaaata tttcttgcgc gcggtcatga cgcgtttcaa atcaacgggc
      541 tcataaggat tagtgaatac acgctcgtca aaatcctgac gcgcgaataa ccacatttcc
      601 ttttgccatt tgccgagtaa ttcatccgca tcctgacctt cttcaatttg gcgcactaac
      661 ctcaggaagc gatgctgagt tttgttccag aagtcgatat ccacaaaact gaaatcaccc
      721 cttgcacctt ttggatcgga gaaccatgct tctttcaatg cactccgtaa cagactcaga
      781 atccgtgaag ccgtttgcgc agccagccgc agcttcggta tctggccttc ttttttattg
      841 agcagtagcg ggaaatggtg ttcgtaccaa cagcgcgctt tcatgttgtc aaaatcataa
      901 ccaaatcccc ataggcccac ttttgcctgt ttcagactgc tggcattaaa gagtttcacc
      961 accaacgcgg gaagttccgt attgttttct gatttacccg tttcgataag gcctaaccag
     1021 tcgcgccaga ttaaaccgcc cggttgtggt ttaacggagt aaaactcgcc accctcttta
     1081 agtggtacac ggtaaggcgt taagggatgc tgccacatgg cataattggc accataattt
     1141 ttggtcgtca tcaggctcag gagcgcgtcg ttctgctcgt cgcaaatatc gcagttgccg
     1201 actgtcgtgg tattaaaatc aatacgtata cgccgcggca ttccccagta cgcctggagt
     1261 ttattgacct gatcatgggt taccaccgca ccgaccagtt cgctggtacg cgtcgggcct
     1321 agccagggga aaatcagatc gtcaaatttt ttgggtagcg gtaagtcggc ttcatcctgc
     1381 ggcatcacgt tgagccacag tttgcgccac aagggtgttt gttgattgcc ctgatattcc
     1441 tgtaattcaa tgagagtcgt catcggccca ccgcctcgta aaccggtgcg atagcctttg
     1501 ccgcccgcgg gtgcattcaa ctgtaaagag aacaaggcca atgctaagca atgggggcac
     1561 agatattccg ttacaccccg cttgataaaa tggtctttat taaacttcgt cgtttgagag
     1621 ccgggaatct caggcagtag cgaagcgacc ggaactttat ctcccgtgag cgcctcaaaa
     1681 tcctgcataa atgaagatga atcaggccca aactggaaag catgttctaa agacagcaat
     1741 gcttctcgta gcttttcagc ttccagtccg tcttcccaga tatcatccca acgacgatga
     1801 tcttttggcg cgaagctggt ttgtagtagt cccagcaaaa actgccaggc cgcaccttgt
     1861 aaatctgctc gcggtgcggc gatatcgaca acattttcat cagccagatc gactggtgcc
     1921 agcttgcctg tcgttccgtc tttgaaacga acaggcaacc acggggttgt cagaagtgaa
     1981 aacgagttca tacgcatcct ctcgtaaaaa agaccatccc tggtcggttg tccatccttc
     2041 catccttttt tgtgtatcaa ctattcagag aatcctgtcg cagacatctc aaccaacctt
     2101 gccaatcaat ccctccgtag ccgaataccc acaactttcg tcatcggtga ctaaaatcac
     2161 gtttgccatc tccggatcat gccgctgttc aacgcaccac tgcctgaacg catccccttc
     2221 cagtaaagaa aactcatccc gatgtttttt ccaccagctt cggcgcactc tgacaacgct
     2281 catttcccat gcgtgtgcac ctgtggcata aggcttcacc acaccgtcaa tacaggtagc
     2341 cagccacagg gaaacagact cttcagccag acgtgtcgac agtttttccg ggaggtaatc
     2401 gttgatattg gcggcatagc cgggcctgaa gttcagtaca aactttttag ccattgcgcg
     2461 atcgcagtaa tatttgccca cttgctcctg ctcgctgcgg gcaaaccctt ccggcattac
     2521 tacgtcctca ccgtagactg attcaatcag aagccgggct gagtgtggca tctgaatagc
     2581 accttgctca cgcagcacac gctgcgtcag ccagattcgc ccatgatcgg gatagacaaa
     2641 tgcactgtta cgcatggcac tgccaaacca ttcgtcaccc ggagagtcgt cccagacggg
     2701 ggccagaatc agcaattcag gagggtgacg ctcgtcttta ccgtcacgct ttaactggcc
     2761 attcatatcg cggatatgtc gctgtaatcg ccctgctcgc tggatcagta aatcaacagg
     2821 ggcaaggtcg gaaatcattt cgtccaggtc gcagtcaacg ctctgctcca ggacttgagt
     2881 acaaatgagg acttttccgg cacgctgtga acagtcttct ttaccaaagc gtgccagcgt
     2941 ctccgtttca attcgctggc gatcgctaaa agcaaagcgg ctatgaaaaa gtgaaaggct
     3001 ggaagcagga atgacgccgc gggcaagtaa ctgacgatga acctggatgg cgtcatcgac
     3061 agaatttcgg atccaggcga tacatttccc ctgacttacc gccgatgcga tacgcgcaat
     3121 acactcttgt tcgctatgaa gccaacccac actcacgctg cgctcaacgt ctttgcgcgt
     3181 cgctacccgg tgtgaatgca catcggattt cgtaacgtgc gtcagccagg ggtaatcatc
     3241 cttttccagg aacggagctt cttgctggcc ctctatacca cgcgcaaagg cagcaacgag
     3301 tttgtcgcgc tgctgttgag ataacgtggc agaaagcaaa atgacgctgt ttccgccacg
     3361 cgcctgccgc tcgatcagcc cttcaagaat gcatgacatg taagcgtcac aggcatggat
     3421 ctcatcagcc agcaggattt tgttactcag tcccagaagc cgcagattat tatgtttgaa
     3481 cggcatcaca gccatcatcg cctgatccag cgtgccgacg ccaatttcag ccagtagcgc
     3541 cttcttgtta ctgtcggcaa accaggccgc acatccctga ctgaacgttt gttcatccgg
     3601 ttcttctgac ccgactaaat caccggacca gagtgattca ttaaagcggt ccattaatgt
     3661 tcgggcactg tgtgccagca ccaggctggg gcgggactct ggcgaataga aagcaagcca
     3721 ggttttgacc agtcgatcgt acatggcatt ggccgttgcc attgttggca ggccaaaaaa
     3781 caaaccctgc gctttccccg cagccatcaa cctgtgcgcc aggataagcg ccgcttctgt
     3841 tttacctgcg ccggttacat cctccagaat aaataactgt ggtcccggct ggctgatatc
     3901 cagatccagc accttttgct gtagcggtgt cggattctca ataaaaggaa acagcgtatt
     3961 aattccggtg aaaggcgcgc tttctgcttt tggaggaaag acggttaagg cgttttgagc
     4021 ctgaactaaa gttttctgcc agtaatcttc aatatccatt gtctgtgcga cgcgtggaaa
     4081 atatcgcgtt gacgatcccg tccagtctgc aagtacgacg gttgcagaga tataccagga
     4141 aagttgtttt ataagttcaa cgccctcgtc atcatcccag aatgtgggga tctctatgag
     4201 cggaaacagt accttgattg caaggagaaa atctcgtgcg gcacctttgt cttcaggcag
     4261 aaaattatcc agttcatcca tacgctcagg gggtcgacca tgatgcccgg tagtcatggg
     4321 catccacatc tccatcaccc gtttaagttt gcgcggagag agtgaagatg caggaagcaa
     4381 ctcctgacat tcacttaaat aatggttcca caaccagtag cccagcgttg aatgagagat
     4441 cttttcgtaa ttctttcttg cgccttccgg gaccttgagt tgtgggtgca ggtaaagttg
     4501 ctgaaaagag cgggcaaatt ttccaatatc gtgccagcac agcagccagg cgaaaaattg
     4561 tgctgcctgc tccgtgtcag cgaaacctaa ttgacgaaaa tagtcagcca gtccgaagca
     4621 attgctttta accattaaat agcccatcgc ggctacatcc agcgaatgcc agcaaagaag
     4681 gtgatagtcg tcgcccccgt ctttctcgcc gcgtcgggtt tttccccaga aatcaaaaaa
     4741 agtcacaata tttttatcct tcagtaaact taaaggatat ttacgcatat attaaaaatt
     4801 atctgtgata tatatcgttc aataaaatat ttacattatg aataatcaaa taattttcat
     4861 ccgtaatata tatgaataat aaacataggc ataatattca cataaacaat ataaattaaa
     4921 aaagggtgca ggagaccaaa ccatcacaat tcagtacgcg gggttttacc cccgcgtggt
     4981 ggctacttct tcgattcgta agcgagaacc gccttaaact ccataccacg ttctctgata
     5041 ctcaactcac acaacgagtc gcctaccatc agcgtgaatc ccagtactgt aaaacacagc
     5101 actatgattg ccacaagggc atattttgtc agcatgtctt gcctccctgc aaagaagaga
     5161 ctaacattcg aattgtttgg gttcgaacgc tggcctcagg ttgatagaaa tatcgcctgg
     5221 ggcttttgtc catctggaac ctcgcgaatg cttaacgcca gacagcctca agcacccaac
     5281 gccattctat acctgttaat tcttccaacg acgttcacgt ttaaaatcag gaatgctgct
     5341 cgcaaaataa aaacgccccc atcatttctg acagaggcgt ttaatttatc cggcaatatt
     5401 tacccttcgt gtaacccaca ttctcttttt aagccaaaga aacgcgtttc ttcttccgcc
     5461 atgccgggtt cccatttacg ggttgtatgg gtatcaccga ccgataaata tccttcatcc
     5521 cataatgggt gatatttcag gccatgtttt tgcaggtact gataaatagt tcggttatcc
     5581 cagtcgataa tcggcagcac tttaaatacg ccacgctgaa ttgccagcac cggtaaattg
     5641 gcacgactgc cggattgttc acggcgcagg ccggcaaacc aggtttgcgc gttcagttct
     5701 tttagcgccc ggttcatcgg ttcgactttg ttgatatcgt tgtacttttc aataccttca
     5761 acgccctgtt cccacagttt gccgtagcgc gcttcctgcc aggcagcgct ttcggtagca
     5821 cggtacactt tcaggttgag tttgagtttg tccgttaact cgtcaataaa gcggtaggtt
     5881 tccgggaaca gataaccggt atcggtgagg atcactggaa tatccgggtg aatctgattc
     5941 accagatgca ggctcaccgc cgcctgaatg ccaaagctgg aagaaagtac atattcaccg
     6001 ggcagattat ccagcgccca ggcaacgcgg ccttcagcgt ccagtttttc cagttcggcg
     6061 ttagtttccg ccagcgccag aatgcgatct accttcggca gttcgttcag ggcgtttaga
     6121 tcgagtttgg acatagattc ctcactgttt gccttgcctg atgcgacgct cacgcgtctt
     6181 atcaggcctt caagaccggg ctgatggtta atcccacaaa tcgcgcgccg gatcgagcac
     6241 cgggcgaatg atgcccgcac gcaccgtaaa gtcgccgaag ccttcacccg cttcgcgctc
     6301 tttcgcccag cgccctatca gttcatcaag cgacgccagg atttccggtt cggtgatgtt
     6361 ttctttatac atccgtggga tacgtgtccc aatgcggttg ccgccaagat gcaggttgta
     6421 gcgacccggc gctttcccca ccagacccac ttccgccagc atcgcgcgac cacaaccgtt
     6481 cgggcagcct gttacacgca tcacgatatg ctcatcgctg acaccatgtt tcgccattaa
     6541 attatcgatt ttgtcgataa aagacggcag gaaacgctcc gcttccgcca tcgccagcgg
     6601 gcaagtcggg aatgacacgc aggccattga gttttcacgc tgcggcgtga cggcattcat
     6661 taacccgctt tctttggcga tcttctcgat cttcgctttc tcgctttccg gcacgccggc
     6721 gatgatcaga ttctggttcg ccgtaatgcg gaaatcacct ttgtggatct tcgcgatctc
     6781 caacaagccg gttttcagcg gacgccccgg ataatcaagg atgcgaccat tttcgataaa
     6841 cagcgtcagg tgccagttat catcaatgcc cttaacccag ccgatacgat cgccgcgccc
     6901 ggtaaattca tacggacgga tcggttcgaa cttgatcccc gcgcgacgtt ccacttctgc
     6961 tttaaacgtc tcgaccccca cgcgctccag cgtgtatttg gttttggcat ttttacgatc
     7021 ggttcggtta ccccagtcac gctgagttgt cacgacggct tccgccaccg ctagcgtatg
     7081 ctccagcggc agatagccaa actcactcgc cgtgcgggcg taggttttct tgttgccgtg
     7141 ttcgatagaa agcccacccc ccaccagcag gttaaagccc accagcttgc cgttttcggc
     7201 gattgccacg aagttcatgt cgttggcatg cagatcgata tcgttctgcg gcgggatcac
     7261 taccgtggtt ttgaatttac gcggcaaata agtctgaccg aggatcggtt cttcatcggt
     7321 ggtggcgact ttttcctgat cgagccagat ctccgcatac gcgcgggtac gcggcagcag
     7381 atgttcagag attttcttcg cccattcata cgcttccgcg tgtagctgcg actcgtaagg
     7441 gttcgaggtg cagagtacgt tacggttcat gtcgttagca gtcgccagcg catcaagacc
     7501 gaccgagtgc agcatctggt gcaccggttt gacgttcttt ttcagaatgc cgtggaactg
     7561 aaacgtctgg cggttggtca ggcgaatgct gccatagatg gtgttttcac cggcaaattt
     7621 atcgatcgcc tgccactgtt tggtggtgat cacgccaccc ggcagacggc agcgcaacag
     7681 catcgcgtgg cgcggctcca gcttctgttc agcacgttcg gcgcggatgt cgcggtcatc
     7741 ctgctgatac atgccgtgga agcggatcag caggaagttg tcgcccttaa agccgccggt
     7801 cagaccgtcg tttaaatctt ccgcaatggt gccgcgcagg tagttgcttt caagcttcat
     7861 gcgctcggcg tctgtcagtt ttccttcgac cactaaaggc cctggatgtt tttcgctcat
     7921 tagtagacat ctcgctgata acggcgctct acgcgcagct cacttaaaaa ttcatccgcc
     7981 gcttcggtgt ccatgccacc aaattcggca atcacttcca gaagtgcctg ttcaacgtct
     8041 ttcgccatgc gattagcgtc gccgcagaca taaatgtggg caccgtcatt gatccagcgc
     8101 cacagctccg cgccctgttc gcgcagtttg tcttgtacgt aaattttttc tttttgatcg
     8161 cgcgaccagg caagatcgat acgcgtcagc acgccctctt tgacgtagcg ctgccattcc
     8221 acctggtaga ggaaatcttc cgtaaagtgc gggttgccaa agaacagcca gtttttcccc
     8281 ggcgcttcgt cggcggcgcg ttgctgcata aaggcgcgga acggcgcaat gccggtgcct
     8341 gggccaatca taatcaccgg cgtttccgga ttggctggca ggcggaagtt atcgttatgt
     8401 tcgataaaga cgcggacttc accctcttct tccacacgat ccgcgaggaa gctggaggca
     8461 ccaccggcac gggcgcggcc ttccacgtcg taacgcacca caccaacggt gacgtgtact
     8521 tcgttctcga cttccgcctg cgaagaggcg atggaataca ggcgcggcgt cagcgggcgc
     8581 agcagattaa tcagcgcttc ggcatccagt tgcgccggag agaaacgcac catgtcaaca
     8641 atcggcgtcg tcgcggcgta atgctgcaac ttcgctttat cgcccaccag cggcagcagc
     8701 gtttcgctgc gggtaagcgt ggcatagttc tcaacaatgt tggcggtgtt gacggtcagt
     8761 tcaaaatgcc actgtagcgc ctcattcagc ggcagggttt tgccctcaac ggtgacaggt
     8821 tcatcgcctt tcagccacag cagttcgaca agttctttca ccagtgccgg atcgttctga
     8881 taccagacgc ccagcgcgtc acccggctgg taacgcaggc ccgagtcacc taagtcaatt
     8941 tcgatatggc gaacgtcttt ttcagagtta cgcccggtaa ttttctggtt aacagagagg
     9001 ctcgccacca gcggcgcgtc tttgctatac gggctggtgt ggatttcatt taccgtgcca
     9061 gtagcgacgg attgcgaagg tgccgcgaca ggcgcacgcg atttaagcgc atcaaccacg
     9121 cgggcgcgcc actcgctggc agcggcctga tattcaacat cggcatcgac acggtcgagc
     9181 aggcgttcac cgcccagttc cgccagcttg ctgtcaaaat ctttcccgga ctggcagaaa
     9241 aattcataag agctatcgcc gaggctaaac acggcaaatg cggtgttttc cagcttcggc
     9301 gcttttttgg agaacaggaa cttatgcagc gcgacggctt cttccggcgg ttccccttcc
     9361 ccttgcgttg acgtcaccac gataagcagt ttttcgctgg cgatttgttt gaatttatag
     9421 tcgcccgcgt tcaccagctt aacgtttagt tttgccgcta ataagtcatc gcgtaacgct
     9481 tcagcaaccc ggcgcgcatt gccggtttgc gaggcggaga taatagttat acccggcatt
     9541 tctgcggcgg gcgctggcgt cgctgcaagc gcagcaggct gctggttgag cacgccccag
     9601 aaatagccag aaacccaggc aagctgggtg ggagttaaat cggtcgtggc cgcctgaagg
     9661 cgtgccagtt gctccgggtt caacggaagc aacgcggaag gtgagacctg tgtcgtcatg
     9721 cgtcgttatg ttccagtaag caaagctgtt tctgcgccct gtcagcgccc ataaaacaga
     9781 agagaaggta aggttaacgg ggtgaacggt gtggattaaa gacgggatag cgataactaa
     9841 taaccaaatc gactaacctg ttttagcaat agtctttaac aacaaaatag attactcaac
     9901 ccaatgaaaa acaaatgaat ttagccaatc attaagataa atcagcgatt ttgcgcaaca
     9961 aagtcgtttt agataatgcg aaaaaacagc ctttccggta ctctacggcg gttttatcgt
    10021 cctgtagaga aattatgatg tccaccacgt tatttaaaga tttcaccttc gaagccgctc
    10081 accgcttacc acacgtcccg gaaggacata aatgtggtcg cctgcacggg cattccttta
    10141 tggtgcgact ggaaattacc ggggaagtcg atccgcatac gggctggatt atcgatttcg
    10201 ctgaactgaa agcggcgttt aagccaacct acgagcgcct cgatcaccat tatctcaatg
    10261 atattccagg tctggaaaac ccaaccagcg aagttttagc aaaatggatt tgggatcagg
    10321 ttaaacccgt tgtgccgctg ttaagtgcgg tgatggtaaa agaaacctgc accgcaggtt
    10381 gtatctatcg cggcgaatga taagagtgtg tcggcggtca atttccctta agtaacgcta
    10441 tgttagggtg ttgtgttctg gatatctggg gcatgacatg gaagacgact gcgacattat
    10501 tattattggt gccggtattg caggcaccgc ttgcgcgtta cgctgcgcgc gagcgggttt
    10561 atccgttttg ttactggaac gcgctgaaat ccccggcagc aaaaatcttt ccggcgggcg
    10621 tttatatacc caggcgctcg cggaactcct cccacaattt catctgaccg cgcctcttga
    10681 acgacgcatc actcacgaaa gcctttccct gttaacgccg gatggcgcaa cgacgttttc
    10741 cagcttacag cccggcggtg aatcctggag tgtattacgt gcacggttcg atccgtggct
    10801 ggttgccgaa gccgaaaaag aaggtgtcga atgcatcccc ggtgcgacgg tggatgcgct
    10861 gtatgaagaa aacggcaggg tgtgtggtgt catttgtggt gacgatattc tccgcgcccg
    10921 ttatgtggtg ctggcagaag gtgccaacag cgtcctggct gaacgtcacg ggttagtgac
    10981 tcgtcctgct ggcgaagcga tggcgttggg gatcaaagaa gtgctgtcgc tggaaacatc
    11041 cgctattgaa gaacgttttc atctggagaa taacgaaggc gcagcgttgc tgttcagcgg
    11101 cgggatctgt gatgacttac ccggcggcgc atttctttat actaatcaac aaacgctctc
    11161 gttagggatt gtttgcccgc tctcttcact tacgcaaagt cgtgttccgg caagcgagct
    11221 gctggctcgc tttaaaacgc atccggcagt gcgcccgctt atcaaaaaca cggaatcact
    11281 ggagtatggt gcgcatctgg tgccagaagg tggcttgcac agtatgcagg tgcaatacgc
    11341 cggtaacggc tggctgctgg tgggcgatac gttgcgcagt tgcgtcaata ccggaatttc
    11401 cgtgcgcggc atggatatgg cgctaactgg cgcgcaggcg gcggcacaaa cgctgataag
    11461 cgcctgccag caccgcgagc cgcaaaatct gtttgcgctt tatcatcaca acgtagagcg
    11521 cagcctgctg tgggatgttc tacaacgtta tcagcatgtt ccggcgcttt tgcaacgccc
    11581 tggctggtat cgggcgtggc ctgcgttaat gcaggatatt tcccgcgatt tatgggatca
    11641 gggtgataaa cctgttccac cgctgcacca gttattctgg cgtcatttac gtcgtcacgg
    11701 cctgtggcat ctggcgggcg atgttatcag gagtctgcga tgtctgtagc ccgtaatctc
    11761 tggcgcgttg ccgatgcgcc gcacattgtt ccggctgact ccgttgagcg tcagacggta
    11821 caatggttga ttaacgcttg tccggctggt cttttttcgc tcacaccaga aggtgactta
    11881 cgtattgact atcgcagttg cctggagtgt ggcacctgcc gtttgctgtg cgacgaaaaa
    11941 acactacaac agtggcgcta tccaccttcc ggattcggca tcacctaccg ttttggataa
    12001 ataataagga taatttatgc ccctcttaca cctgctccgc cagaatccgg tgattgctgc
    12061 cgttaaagat aatgccagcc tgcaactggc aatcgactct gaatgccagt ttatttccgt
    12121 gttgtacggc aatatctgca ccattagtaa tatcgttaaa aagattaaga acgccgggaa
    12181 atatgctttt attcatgttg atttgctgga aggcgcgtca aataaagaag tggtgattca
    12241 gtttttgaaa ctggtgaccg aggcggacgg aattatcagt accaaagcct caatgctgaa
    12301 agcagcaaga gcggaaggtt ttttctgtat tcatcgactg tttattgttg attcgatttc
    12361 gtttcacaac attgataagc aagttgcgca atcgaatccg gattgtattg agatcctgcc
    12421 tggctgtatg cccaaagtgc tgggctgggt gacggagaaa atccgccaac cgctgattgc
    12481 cggcgggctg gtgtgcgatg aagaagatgc gcgtaatgcg attaacgcgg gtgtcgtggc
    12541 gctttccacc acgaataccg gggtctggac gttggcgaaa aaattgcttt aacgggataa
    12601 tcgtaacgaa ttgaatttgt gtgatttgta ggctgcacgc cacatccgac attcagcgcc
    12661 tgatgcgacg cttgacgcgt cttatcaggc ctacaagtcc ccaggccaga accgtaggca
    12721 ggatcatccg gcaacacgtt attggcaatc tgcgtgaatg tttgtcacca gtgcctccag
    12781 caccactttc cagtcatcaa ccacgccaac atctgcctgt gaaaataccg ctgcgctggc
    12841 gtcatgatta atcgccacca caaacttgct attgcgtacg cccgccatta acgctgctgc
    12901 gccggaagca ccaacaacaa tacacacctc aggtgccagc agatgtccgg aaataccaat
    12961 gactttttcg gcatcaacgc caccgttcat cactcgtgcc cgactgtagc ccacttcggc
    13021 tcccagcttt tctgccagca tagcgatttc ctggttatct gcctctccgc cctgccccac
    13081 caccagcacc cgtctggctt cagctaatgg gtcgcgggta acatttttca ggttttctag
    13141 gctaaccagc cagtcgggaa gcgcgccggg gacaatgttc agttgctgca tcccagagga
    13201 caacgtggcg tttttatctg ctcccgcctg acgtgccagc gaaaggcata gcgggcgttt
    13261 gtctgtttgc agcgtcgccg tcaacgcatt tccccagtgg gatttgcgca cgcttacggt
    13321 tggaatatct aacgaagtca cctggcagat gcttgcacca tgtaaacgcc aggccagccg
    13381 ggtcgacagt tcatcgccaa acgttcccgg cggaaagaga acaacgtccg caggcgttcg
    13441 ctgccactgt tccaccaacg catccagcac ctgttccgcc actacgggct gaggttcaat
    13501 ctgccagtgc gccagggtac aaccgctgaa atcctgttct gccagccagc tcgctattgc
    13561 ggcattctcc tgatttattg tcacaatggc gatattcatg gctgcatcct ttggcgtaaa
    13621 tagtcctgcc atagcttttg cgctttttct gctaccgttt gcccatcaat cagcgtcgct
    13681 ccgcgtcgtt gctctgctct tgccagttgc agacactgca tcgctggcaa ttcgacagca
    13741 acagttttac ggataatttc tgctttcccc gcagccattc gctggcgcat tcccggtact
    13801 ggcaaggcca cttcaccgca ctgacgcacg gcaatcaccg caggcaagcg aacccggcaa
    13861 cagcgcaccc cattttcagt acgctgttcg agggtgataa acggcgcgtc gagtgtgaaa
    13921 cgctccacct gggtgaaaca gggccagccc agcatttccg ccagcaaaaa tggcgtttgc
    13981 ccattctgtc cttcgctgct ttggcagccg gtaatgatca gatccagcgg attctgatgc
    14041 tgccactcgg caatatggcg agcgacaaat tccggcgcaa agcgcagatc tgccgccgtc
    14101 tccagcaata cagcttcctc aaacccaaga gccatgagat agcgcagcca gtgcaacgcc
    14161 cgttcatccc ccatgctcaa cgcggttaaa gacatcggag taccattttt tctctgcgcc
    14221 aacagcagcg cggcggcagc ctgttcatca gcaccgagta aacttcgcag tagcgaaaca
    14281 tctggtccaa tattaccctg agccgccgcc tgccactctt tttccgccag cattccggca
    14341 tccggttcgg ctttaaacgc taacagaatg ttcattcgca cctcctacat ttcccgccgc
    14401 caccagcggg agtgctttgg tttctggtgc ccatagccag gtcacaacaa aaccaaccaa
    14461 caacactgcc gccaggagca ataaggtggc ttgcattccc cactgcgcca gcacccacgg
    14521 cagcaggcca gtacttaccg ccgcccccaa ccgactcatg gcggtggcaa aaccgacgcc
    14581 cagcgagcga atgtcagtag gaaaactttc cgcaggcaaa atgcccacca gattactgac
    14641 tgccgaaatg gtggtgctga agagaacaaa aagcagcagc gttaatgaac tgccggaagg
    14701 caaacaggcc atcactacca gcgttgccgc cagcagcaaa aaacttccca gcaaaaactt
    14761 gcgatgtgcc agcaggtgcg tcagaactaa tcccagcagc gcgcccacaa ttaacaacgc
    14821 attaagcatc aggctggcgg tcagcgcatc ctccagacca atagtctggg cgatggtcgg
    14881 cagccaggta taaatcacaa accatgggat tacgaggcag acaaagaaga cgctgttaaa
    14941 cgccgtgcgt cgccagtagc gcgaagaaaa caaggttttg atgtgtttat gggtcgccgc
    15001 tgccacttca tcgcccagta aaacatgtgg ccccaaatag cgatgcacaa tagcgtgagc
    15061 ttcagcaaaa cgtccctggc gcagtagcca gcgtggcgat tccggcgtgc cccaacgtag
    15121 cagcgtaatc agcaaggctg gtaacgccgc tgatgccagc agccagcgcc aggcctccgg
    15181 gttttcggaa ataaagtgat gtccggcaat acttgccaac acatagccta cggtccacac
    15241 cacgctgaat gcgcccagta aaataccgcg atggcggcgc ggggaaaatt cagccagcaa
    15301 ggtgtgaccg actgagtaat cgcctcccag accaatgccg atcaaaatgc gcagcccaat
    15361 aagatgctct ggcgtggtgg caaaaaattg caagaacgaa gcgagcgtaa tcagcaaaaa
    15421 gctgaaggtg aagatttttt gccgaccaat atggtcggag atccatccaa gaaccaggct
    15481 gccaagaaac aaaccaagga gtgctgagcc gccaatcatt cccgccataa acggcgtcag
    15541 ttgcatggcg ggcgtaagct gaataatggc gtaaccaatg acgccgagga cataaccgtc
    15601 ggtcaggtgc gcaccgaaag tgagcgcagc aatgcggcag tgaaaacggt taagtggtaa
    15661 atcatccatt cgcaccggtg aagtgttcat ctttacccct tcattgtccg gtaataagaa
    15721 cggcttctcg cctgagaagc cgaaaaggca acgccccgtc atcgcggaac gctgcctcac
    15781 acattatttt tcaatcggat agatagtacc cgtattcata atgccgttag gatcgaactg
    15841 ttttttcagc ccttccagca acgtccacgc gctgccgtgt tccagtttgc tccagtgaac
    15901 gcgatgttta ccaataccgt ggtggtgcac catcgaaccg ccgaggcgaa tggtttcttc
    15961 gcaaatgatt ttgttgagcg gattgtggta cttgtcgatt tcctcttccg gcttacagtc
    16021 aacgacgttg tagtcgtaga cgaagtacat gttggtgccg ttctgatagc tatgagagga
    16081 gtgaccgccc agcatggtga tgtcgtcggc gtgcgggaac tcggtgcgaa tacggttaat
    16141 aacgctttcg tagatttcgt ggatgcagct ccagcagccg gacacttcgg tggtaaagcc
    16201 catgttgccg gttttgagga tctgcacacg ttcggcagcc actttatccg gtccccagtt
    16261 caggttgtta aaccaggttt cgatcagctt gctgtccacg cgctggcact gcgggtagcg
    16321 cgccacgatt tcagcaatcc cttcacccgt cgccttcgca atgcgagggt taccttcagc
    16381 cataaagatc agcacacatt taccgtcggc aaaatgggtg aagtgttggg tgccatcttc
    16441 aacgtcatac aaacgagcga tcgacggacg atacccttcc accatgattt cacgcaggat
    16501 gttgaagccg gttttcatgt cttccaggat atagccgtag aagaggttgt tttccggggt
    16561 gaacttaaag attttcactg ttacttcagt gatatagcac aatgcacctt cgttgccgat
    16621 gataatgtga cgaatgtccg ggcctgccgc gcggcgtggt acgtttttaa tacgtgtgac
    16681 ggtgccatca gccaatactg cttccagacc aacgaccata tcttcgattg cgccgtagag
    16741 tgtggagaac tgcccgatac tgcgggttgc taccaggccg cccatctgcg ccagcggttt
    16801 tgattgcggt gaatgccccg tggtgtaacc tttttcacgc aacgcgtttt ccagcacctc
    16861 cagcggaacg ccacattgcg ccgtggcctg catattctca atatcaacat taatgatttg
    16921 attcatggca gagccgtcga gcaccaccga gttttccaca acggtttcca gcccgccttc
    16981 agtggcggaa gcaccggtac gcggcacacc gttaattttg tgcgcattca taaaattcag
    17041 cacacgggac acttgctctg tggaaccgag ttttacgacc gctgccggaa taggcaaagt
    17101 ataaatgcca tgaatatccg gaaatttgcg aaaacggtca atactgtttt tctttaatac
    17161 tgtttcatcg gtaattacgc gatctgcacc aacaatttcc tttagctggt cgacaatcgc
    17221 tgcgcgagat aaagacatag taattccttc ctgataataa aaatggaata atcattaata
    17281 agagaaaaca gaaaccaccg ttatggatta gcgcactaaa taaccgccat caaccactaa
    17341 taaatgtccg ttgacataat tcgatgccgg acttgcgaga aatatggctg tgcccattaa
    17401 atcctgagta tcgccccaac ggtttgctgg aatatgatca agaacgcgct gattggtttc
    17461 tggattactg cgtgtcgcca gggtgatatc ggtagcgtaa tatccagggg caataccatt
    17521 tacctgaata ttatattgac ctagttcatc acaataagct ttggtgaatc cggcaagagc
    17581 atgtttagtg gcagaatatg caggtgacca ttgtccacct aagtaagaga ataatgaaca
    17641 gatattaatg attttaccgc ttttctgcgg gatcataatt tttgcagctt catagcttaa
    17701 ctcgaatgcg gcggtcaggt tcacgtcaat catcgggtcc cagtcggcac gaccaaagtc
    17761 cagcacctta ttcagcttac aaataccggc attgttaacc agaatatcta cggtaccgaa
    17821 gcgctcacag caggcagcga taatcttctg cggcgcgcct tctgcggtga tatccacctg
    17881 cataaaatca acttcgacac cctgtttttc aatcatttcc tttgtttcgc cgttatcttt
    17941 gacgaagcta gggataaaga catttgcgcc agctttggcc aacgccatgg caaatgcctg
    18001 gcctaaaccg ctattcccgc cggtaacaat tgctgtttta cctttcaggg agaaaaaatc
    18061 cattgagaac gcattgagag attcgattga catagttaac tccaatttcc gtaaagcaaa
    18121 aaaaagaaag gtaacttccc ccgcgagagg gaagttacct ttctcattat ctctggtaac
    18181 tggagataat ttttagcata agggcataag cgctaatgtg atcgtaatca cagtgtgata
    18241 ttcgtattgt caaataattt taaaaagcag aagcgtgact actctcacac aacaccactg
    18301 ctttccatgt tatttcttat tgcaattact agagacctga gaaaagtaat ccacctgaaa
    18361 gggcggctta cttttctctt ttttttgtct attacaaatg ggaatacgat atgcaacaca
    18421 actcatatcg ccgttggata accctcgcga taattagttt tagcggcggc gttagtttcg
    18481 acctggctta tttacgttat atttatcaaa ttcccatggc gaaatttatg ggattcagca
    18541 ataccgagat aggtttaata atgagtacct ttggtattgc agctattatt ctttatgcgc
    18601 ccagtggcgt tattgccgat aaattctcac atcgcaaaat gattacttcc gcgatgatca
    18661 ttaccggatt actgggtctg ttaatggcaa cttatccacc gctgtgggta atgctctgta
    18721 ttcaggttgc ctttgcgata acgacgattt taatgctgtg gtcggtgtcg attaaagccg
    18781 catcgttgct gggcgatcat agcgagcaag ggaaaattat gggctggatg gaagggctgc
    18841 gcggcgtcgg tgtaatgtcg ctggcggtgt ttaccatgtg ggtcttttct cgctttgcac
    18901 cggatgacag cgccagcctg aaaacggtca ttatcatcta tagtgtggtt tacatcttgt
    18961 tggggattct gtgctggttt tttgttagcg ataacaacaa cctgcgcagt gccaataacg
    19021 aagaaaaaca atcattccag cttagcgaca tcctggctgt tttgcgcatc agcaccacct
    19081 ggtattgcag catggtgatt tttggcgtct tcaccatcta cgccattctg agttactcca
    19141 ccaactatct caccgaaatg tacggcatgt cgctggtggc ggcgagctac atggggattg
    19201 tgatcaacaa aatcttccgc gcgctgtgtg gtccacttgg cggcattatc accacctaca
    19261 gtaaagtgaa atcccctacc cgcgtaattc aaatcctttc catcatcggc ctgctggcac
    19321 tgactgccct gcttgttacc aactctaacc cgcaatcggt cgctatgggg attggcctga
    19381 ttttactact gggattcacc tgctacgcct cacgtggact gtactgggcc tgccccggcg
    19441 aagcgagaac accgtcttac attatgggca ccacggtagg tatttgttcg gtgattggat
    19501 tcctgccgga tgtcttcgtt tacccgatta tcggccactg gcaagacacc ctgcccgctg
    19561 cagaagccta ccgcaacatg tggctaatgg gcatggcggc gcttgccatg gtgatcatct
    19621 ttaccttttt gctgttccaa aaaattcgta ctgctgatag cgcccctgct atggctagca
    19681 gcaagtaagt ccagaaaatt acaggagaaa aatcatgtcg aaaaaataca tcatagggat
    19741 tgatggcgga agtcagagca caaaagtggt gctgtatgat ctggaaggta atgtggtttg
    19801 cgaaggcaaa ggcttattgc agccgatgca cacgccagat gccgataccg cagaacatcc
    19861 tgacgacgat ttatgggcat cattatgttt tgccggtcac gatttgatga gtcagtttgc
    19921 cggaaataaa gaagatattg tcggtattgg tctgggatcc atccgttgct gccgtgcgtt
    19981 attgaaagcc gatggcacgc cagctgcgcc gttgattagc tggcaggatg cacgcgttac
    20041 acgtccctac gagcacacca atcctgatgt ggcatatgtc acctcttttt caggttatct
    20101 gacacaccgt ttaaccggcg agtttaaaga caatatcgcc aactattttg gtcagtggcc
    20161 ggtggattat aagacctggg catggagcga agataccgcg gtaatggaga agtttaatat
    20221 tccccgacag atgctttttg atgtgcaaat gccgggcact gttcttgggc atatcacacc
    20281 acaagccgcg ctggcgacac atttcccggt tggactgccc gttgtttgta ccaccagtga
    20341 taaaccggta gaagcactgg gagcagggtt actggatgat gaaacggcgg taatttctct
    20401 tggcacttac atcgcgttga tgatgaacgg caaagcactg ccgaaagatc cggtagcgta
    20461 ctggccgatt atgtcttcta ttccgcaaac cttgctgtat gaaggttacg gtattcgcaa
    20521 aggcatgtgg acggtgagct ggctacgcga catgctaggc gagtcgttaa ttcaggatgc
    20581 caaagcacag gatctttcac cggaagattt gctcaacaaa aaagcgtctt gtgtgccgcc
    20641 tggctgtaat ggtctgatga cggtgctgga ctggctgacc aatccgtggg aaccatacaa
    20701 acgcgggatt atgatcggct ttgattccag catggattac gcatggatat atcgttcgat
    20761 actggaaagc gtggcgctga cgctgaagaa caattacgac aatatgtgta atgaaatgaa
    20821 tcactttgca aagcatgtga tcattactgg cggcggttcg aacagcgatc tgtttatgca
    20881 gatttttgcc gacgtgttca accttccggc acgccgaaac gccattaatg gttgtgcaag
    20941 tctgggggct gcgatcaata cggcggcagg tctggggcta tacccggatt acgcaacggc
    21001 tgtcgacaaa atggttcgcg tgaaagatat ctttatgcct gttgagagca acgccaaacg
    21061 ctacgacgcg atgaataaag gcattttcaa agacctaacc aagcacactg atgtgatcct
    21121 gaaaaaatcg tatgaagtga tgcatggtga gctggggaat gaagattcga tccagagctg
    21181 gtcgaatgcg taagtgagat aaacagtgcc gggtagatga ttaatcccgg catattcacg
    21241 tttccaccac aaaataccgg gtaaccactg ctgaatattg ttctctcctg agcagcgttt
    21301 atgctttaat tgctaaaccg atttaattag tttagcaatt agtatattgg agcctttatg
    21361 agcgcaagag tatgggtact cggtgatgcg gttgttgatt tattacccga aagccagggg
    21421 agactactac agtgtcctgg cggggcgcct gctaatgttg cagtcggtat cgcaaggctg
    21481 ggggggaaaa gtgcctttat tggcaaagtt ggcgatgatc ctttcggtcg ctttatgtat
    21541 cagacactga gtacagaaaa tgttgataca cattatatgt ctcttgatcc tcaacaacgc
    21601 acctcaattg tggctgtagg acttgatgag caaggagaaa gaaactttac ctttatggta
    21661 cgcccaagtg ccgatctttt tttacaacct ggtgaccttc ctgcatttgg gccgggtgaa
    21721 tggctccatc tttgttccat tgcgctcagt gcagaacctt cccgaagtac cgcatttctg
    21781 gctatggaga aaatacgtca ggctggcgga aacatcagtt ttgatcccaa tatccgcagc
    21841 gatctctggc agagtgaagc gctattaagg aaataccttg atcgcgcact ttcgctggcg
    21901 aatatcgcta aattgtccga agaagagttg ctattcatca gtggcgaaag ccaggttcag
    21961 caaggcgcat attcattagt acaacgttat tcgttgactt tattgcttat tacacaagga
    22021 aaaaatggcg tacttgtgta ttttcagggg cagtttatcc actatcccgc caaacctgtt
    22081 tctgtcgtcg atacgaccgg ggcaggagat gcttttgtcg ctggattact tgcaggtctg
    22141 gctgattctg gaataccaac aaataccaga cagcttgaac gaatcattgc acaagctcag
    22201 atttgtggtg ctctggcgac cacggctaaa ggcgcgataa ccgccttacc ccgacaacac
    22261 gatctccctt cacaatagtg atcctgctgt tgtatctgta acccgatttg tcgtgcctgt
    22321 cacatttact aaatcggttt agcgaaaata attgctctgg ctaaaaagtc agctttatta
    22381 tcaacaccta aaccggttta gcattttagc aatttttata attctctgat gggacgcgac
    22441 aaattatgta taaaaaaaca actttggcaa tgttaattgc tttgctgacc ggtgctacaa
    22501 cggtacatgc gcaaacggat attagcagta tcgaatctcg actggcggcg ttggaacaac
    22561 gtttaaaaaa tgcggaatcc cgcgcccagg cggcagaagc aagggccaaa acagctgaat
    22621 tacaggttca gaaactggct gaaacacaac aacaaaatca gctaacaact caagaagtag
    22681 cacagagaac agttcagctc gaacagaaat ccgcagaaaa cagtggtttt gagtttcatg
    22741 gctatgcccg ttccgggtta ctgatgaatg atgccgcttc cagtagcaaa agtgggccgt
    22801 atctgactcc cgcaggtgaa actggtggag ctgttggccg tctgggaaat gaagccgata
    22861 cctatgtcga gttaaatgta gaacataaac aaacactaga taacggtgcg accacacgct
    22921 ttaaagcaat gttggctgac ggacaaagag attacaacga ctggactggc ggctccagta
    22981 acctgaatat ccgacaggct tttgccgaac tgggcgcatt accaagtttt accggagcat
    23041 tcaaagacag tactgtctgg gctggtaaac gctttgatcg cgacaatttt gatattcact
    23101 ggttagactc cgatgtcgta tttttagcgg gaacgggcgg cggtatctat gacgtaaaat
    23161 ggaacgatac attccgcagt aacttttctc tctacggacg taatttcggc gatcttgatg
    23221 atatcgacaa taacgttcag aactacatcc tcaccatgaa tcattatgca ggccccttcc
    23281 agttgatggt tagcggatta cgggcaaaag ataatgatga tcgaaaagat gccaatggtg
    23341 atctcattca aactgatgct gcaaatactg gcgtacatgc gttagttggt ctgcacaatg
    23401 acactttcta tggcctgcgt gaagggacgg caaaaacagc actgctatat ggccatggcc
    23461 tgggtgcgga agtcaaaggg attggctccg atggcgctct gctgtctgag gcgaatacct
    23521 ggcgcttcgc atcttacggc acaacacctc tgggaagcgg ttggtatgtt gcgccagcaa
    23581 ttctcgcaca aagcagtaaa gatcgttacg tcaaaggcga tagctacgaa tgggtgacct
    23641 tcaatacacg tctgatcaaa gaggtaacac agaattttgc tctggccttt gagggtagct
    23701 atcaatatat ggatctgaag ccaaaggggt atcaaaacca caacgccgta aacggcagct
    23761 tctataaact cacctttgct ccaactctaa aagctaacga tatcaataat ttctttagcc
    23821 gtccggagct tcgcctgttt gccacctgga tggactggag cagcaaactt gatgattttg
    23881 ccagcaatga cgctttcggc agcagtggtt tcaatactgg tggagagtgg aattttggtg
    23941 tccaaatgga aacctggttt taataccaga cagcctcacg aatgtgaggc ttttactgaa
    24001 ataacaatga attagagggt tatatggatt tcgataaaat cgcccaatcg ttgcttcctt
    24061 tattaggtgg taaggagaac atcgccagtg cagcacattg cgccactcgc ctacggctgg
    24121 tactggttga cgacacactt gccgatcaac atgccattgg ccagattgat ggagtgaaag
    24181 gttgttttcg taattcaggg caaatgcaga tcatctttgg caccggcgtg gttaataaag
    24241 tctatgctgc ctttattcag gttgcgggta ttagcgaatc cagtaaagca gataccgctc
    24301 gactcgccgc tcaaaaactc aatccttttc agcgaatagc acggctgctt tctaatattt
    24361 ttgtccccat cattcctgcc attgttgctt ctggtttatt aatggggctt ctgggaatgg
    24421 tgaaaacata tggctgggtg aatgccgata atgcgattta tatcctgctg gatatgtgca
    24481 gttcagccgc atttattatt ctccccatcc tgattggctt tactgctgct cgtgaatttg
    24541 gtggaaatcc ttatcttggc gcgacattag gagggattct gactcacccg gcactcacaa
    24601 atgcctgggg cgttgctgcg ggatttcaga caatgaactt cttcggtttt gaaatcgcca
    24661 tgattggcta tcagggaacg gttttccccg tgcttctggc agtatggttt atgagcatag
    24721 tggaaaaaca gttacgcagg tttatccctg atgctctgga tctcattctg acgccatttc
    24781 tgactgtcgt catttctggc tttatcgctc ttttaattat tggtccggca gggcgagcgt
    24841 taggtgatgg tatctctttc gttctcagca cgctgattgc acacgctggc tggttagcgg
    24901 ggttgttatt cggcgggcta tattcagcga ttgttattac gggtattcat catagcttcc
    24961 acgcaattga agcaggactt ttaggtaacc ctgcaatcgg tgttaatttc ctgctgccta
    25021 tttgggcaat ggcaaacgtt gcgcaaggcg gtgcatgtct ggcggtatgg tttaaaacca
    25081 aagatacaaa aattaaagca atcaccctac cctcggcttt ttccgcaatg ttagggatta
    25141 ctgaagccgc tatttttggt atcaaccttc gttttgttaa accttttatt gcggccttaa
    25201 ttggtggcgc tgccggcggt gcctgggttg tctctgttca cgtttatatg actgccgttg
    25261 gtctgaccgc aatacccggc atggcaatcg ttcagccaac atcgttggtt aattatatta
    25321 ttggaatggt gattgctttc gctgtcgctt ttagtctctc tttattgctc aaatacaaaa
    25381 cagacgagga gtaatgtcga tgccttcaca aatgcccgcg attttacagg cagtgatgaa
    25441 aggtcagtcc aaagcacagg cagatgccca ctatccttgt tggcatctcg ctcccgtcac
    25501 cggactaatg aacgatccaa atggcttttg ctggtcagga ggccgctatc atctgtttta
    25561 tcagtggaat ccattggatt gtaatcataa gtacaaatgt tgggggcact ggagttcgac
    25621 ggatttattg cactggcagc atgaaccatt agcgctaatg cccgataaag agtatgaccg
    25681 taatggttgt tactcaggaa gcgcggttaa taatcagggc gttctcacac tttgctatac
    25741 cggtaatgtt aagtttgatg atggcagcag aacggcatgg caatgtctgg ccaccgaaaa
    25801 taaccagggc gggtttgata aattaggccc tgtcataccg ttacctgatg gttacaccgg
    25861 acatgttcgt gatccaaaag tctggaagca caacagccga tggtacatgg tgcttggcgc
    25921 gcaagataaa gagaaacggg ggaaagtgct gctgtattcc tctgtcgatc tctatacctg
    25981 gtcttttcat ggagaaattg ctggtaatgg tctgaatgaa attgataacg caggatacat
    26041 gtgggaatgt cctgatcttt tcgcccttga tggtgaatac attctcctct gctgccccca
    26101 gggaatggcg cgcgaacatg agcgctatct gaatacctat ccctgcgcct ggctacatgg
    26161 acagtttgat tacgagacag gcaaatttac gcatggcgct ttctcagagc tggatgcggg
    26221 atttgaattc tatgcaccac agacgatgga agccccggac gggcgacgat tactggtcgg
    26281 ctggatgggg gttcctgatg gcgaagagat gttgcaacca accagaaagc atcattggca
    26341 gcatcaaatg acctgttttc gcgaactctc attccagaag ggaaaattat ttcagatgcc
    26401 aatcagggaa ctcgcgcaac tgcgtg
//
LOCUS       DAMZFT010000063        26410 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.063, whole genome shotgun sequence.
ACCESSION   DAMZFT010000063 DAMZFT010000000
VERSION     DAMZFT010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 26410)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26410)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26410
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.063"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..450
                     /locus_tag="PN977_002705"
     CDS             <1..450
                     /locus_tag="PN977_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6042723.1"
                     /translation="GCYTAMALLPMSDAGKQLAEREHYRLRRDAQALAQWNGEMLPVD
                     PLNDAVLSDDDWLELAGFAFAHRPLLTSLGCLMRLLQTSEMALPALRGRLQKNASDAQ
                     LCTTLKLSGRKLLLVRQREEAAQALYALDDVRTERLRDRITQWQFFH"
     gene            524..1222
                     /gene="ypfH"
                     /locus_tag="PN977_002706"
     CDS             524..1222
                     /gene="ypfH"
                     /locus_tag="PN977_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HDI6042724.1"
                     /translation="MKHDHFVVQSPDKPAQQLLLLFHGVGDNPVAMGEIGSWFAPLFP
                     DALVVSVGGAEPSGNPAGRQWFSVQGITEDNRQARVDAIMPTFIETVRYWQKQSGVGA
                     NATALIGFSQGAIMALESIKAEPGLASRVIAFNGRYASLPETASTATTIHLIHGGEDP
                     VIDLAHAVAAQEALISAGGDVTLDIVEDLGHAIDNRSMQFALDHLRYTIPKHYFDEAL
                     SGGKPGDDDVIEMM"
     gene            complement(1303..1503)
                     /locus_tag="PN977_002707"
     CDS             complement(1303..1503)
                     /locus_tag="PN977_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpfN family protein"
                     /protein_id="HDI6042725.1"
                     /translation="MDWLAKYWWILVIVFLVGVLLNVIKDLKRVDHKKFLANKPELPP
                     HRDFNDKWDDDDDWPKKDQPKK"
     gene            complement(1531..2658)
                     /gene="dapE"
                     /locus_tag="PN977_002708"
     CDS             complement(1531..2658)
                     /gene="dapE"
                     /locus_tag="PN977_002708"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020078199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HDI6042726.1"
                     /translation="MSCPVIELTQQLIRRPSLSPDDAGCQALLIERLQAIGFTVERMD
                     FADTQNFWAWRGQGETLAFAGHTDVVPPGDADRWINPPFEPTIRDGMLFGRGAADMKG
                     SLAAMVVAAERFVAQHPNHTGRLAFLITSDEEASAHNGTVKVVEALMARNERLDYCLV
                     GEPSSIEVVGDVVKNGRRGSLTCNLTIHGVQGHVAYPHLADNPVHRAAPFLNELVAIE
                     WDQGNEFFPATSMQIANIQAGTGSNNVIPGELFVQFNFRFSTELTDEMIKAQVLALLE
                     KHQLRYTVDWWLSGQPFLTARGKLVDAVVNAVEHYNEIKPQLLTTGGTSDGRFIARMG
                     AQVVELGPVNATIHKINECVNAADLQLLARMYQRIMEQLVA"
     gene            complement(2662..3018)
                     /locus_tag="PN977_002709"
     CDS             complement(2662..3018)
                     /locus_tag="PN977_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC family reductase"
                     /protein_id="HDI6042727.1"
                     /translation="MVTLYGIKNCDTIKKARRWLEANNIDYRFHDYRVDGLDSEFLNG
                     FINELGWEALLNTRGTTWRKLDETTRNKITDAASAATLMTEMPAIIKRPLLCAPGKPM
                     LLGFSESSYQQFFHEV"
     gene            3399..3458
                     /gene="ypfM"
                     /locus_tag="PN977_002710"
     CDS             3399..3458
                     /gene="ypfM"
                     /locus_tag="PN977_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YpfM"
                     /protein_id="HDI6042728.1"
                     /translation="MIERELGNWKDFIEVMLRK"
     gene            complement(3557..6670)
                     /gene="acrD"
                     /locus_tag="PN977_002711"
     CDS             complement(3557..6670)
                     /gene="acrD"
                     /locus_tag="PN977_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease AcrD"
                     /protein_id="HDI6042729.1"
                     /translation="MANFFIDRPIFAWVLAILLCLTGTLAIFSLPVEQYPDLAPPNVR
                     VTANYPGASAQTLENTVTQVIEQNMTGLDNLMYMSSQSSGTGQASVTLSFKAGTDPDE
                     AVQQVQNQLQSAMRKLPQAVQNQGVTVRKTGDTNILTIAFVSTDGSMDKQDIADYVAS
                     NIQDPLSRVNGVGDIDAYRSQYSMRIWLDPAKLNSFQMTAKDVTDAIESQNAQIAVGQ
                     LGGTPSVDKQALNATINAQSLLQTPEQFRDITLRVNQDGSEVRLGDVATIEMGAEKYD
                     YLSRFNGMPASGLGVKLASGANEMATAELVLNRLDELAQYFPHGLEYKVAYETTSFVK
                     ASIEDVVKTLLEAIALVFLVMYLFLQNFRATLIPTIAVPVVLMGTFSVLYAFGYSVNT
                     LTMFAMVLAIGLLVDDAIVVVENVERIMSEEGLTPREATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGTTGAIYRQFSITIVAAMVLSVLVAMILTPALCATLLKPLKKGEHHGQ
                     KGFFAWFNQMFNRNAERYEKGVAKILHRSLRWIVIYVLLLGGMVFLFLRLPTSFLPLE
                     DRGMFTTSVQLPSGSTQQQTLKVVEQIEKYYFTHEKDNIMSVFATVGSGPGGNGQNVA
                     RMFIRLKDWSERDSKTGTSFAIIERATKAFNKIKEARVIASSPPAISGLGSSAGFDME
                     LQDHAGAGHDALMAARNQLLALAAENPELTRVRHNGLDDSPQLQIDIDQRKAQALGVA
                     IDDINDTLQTAWGSSYVNDFMDRGRVKKVYVQAAAPYRMLPDDINLWYVRNKDGGMVP
                     FSAFATSRWETGSPRLERYNGYSAVEIVGEAAPGVSTGTAMDIMESLVKQLPNGFGLE
                     WTAMSYQERLSGAQAPALYAISLLVVFLCLAALYESWSVPFSVMLVVPLGVIGALLAT
                     WMRGLENDVYFQVGLLTVIGLSAKNAILIVEFANEMNQKGHDLFEATLHACRQRLRPI
                     LMTSLAFIFGVLPMATSTGAGSGGQHAVGTGVMGGMISATILAIYFVPLFFVLVRRRF
                     PLKPRPE"
     gene            complement(6834..8534)
                     /gene="narQ"
                     /locus_tag="PN977_002712"
     CDS             complement(6834..8534)
                     /gene="narQ"
                     /locus_tag="PN977_002712"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarQ"
                     /protein_id="HDI6042730.1"
                     /translation="MIVKRPVSASLARAFFYIVLLSILSTGIALLTLASSLRDAEAIN
                     IAGSLRMQSYRLGYDLQSGSPQLNAHRQLFQQALHSPVLTNLNVWYVPEAVKTRYAHL
                     NANWLEMNNRLSKGDLPWYQANINNYVNQIDLFVLALQHYAERKMLLVVAISLAGGIG
                     IFTLVFFTLRRIRHQVVAPLNQLVTASQRIEHGQFDSPPLDTSLPNELGLLAKTFNQM
                     SSELHKLYRSLEASVEEKTRDLHEAKRRLEVLYQCSQALNTSQIDVHCFRHILQIVRD
                     NEAAEYLELNVGDNWRISEGQPNPELPMQILPVTMQETVYGELHWQNSHVSSSEPLLN
                     SVSSMLGRGLYFNQAQKHFQQLLLMEERATIARELHDSLAQVLSYLRIQLTLLKRSIP
                     EDNATAQSIMADFSQALNDAYRQLRELLTTFRLTLQQADLPSALREMLDTLQNQTSAK
                     LTLDCRLPTLALDAQMQVHLLQIIREAVLNAMKHANASEIAVSCVTAPDGNHTVYIRD
                     NGIGIGEPKEPEGHYGLNIMRERAERLGGTLTFSQPSGGGTLVSISFRSAEGEESQLM
                     "
     gene            8740..10719
                     /gene="aegA"
                     /locus_tag="PN977_002713"
     CDS             8740..10719
                     /gene="aegA"
                     /locus_tag="PN977_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent uric acid utilization protein
                     AegA"
                     /protein_id="HDI6042731.1"
                     /translation="MNRFIMANSQQCLGCHACEIACVMAHNDEQHVLSQRHFHPRITV
                     IKHQQQRSAVTCHHCEDAPCARSCPNGAISHVDDSIQVNQQKCIGCKSCVVACPFGTM
                     QIVLTPVAAGKVKATAHKCDLCAGRENGPACVENCPADALQLVTDAALSGMAKSRRLR
                     TARQEHQPWHASTAAQEMPVMSKVEQMQATPARGEPDKLAIEARKTGFDEIYLPFRAD
                     QAQREASRCLKCGEHSVCEWTCPLHNHIPQWIELVKAGNIDAAVELSHQTNTLPEITG
                     RVCPQDRLCEGACTIRDEHGAVTIGNIERYISDQALAKGWRPDLSHVTKVDKRVAIIG
                     AGPAGLACADVLTRNGVEVTVYDRHPEIGGLLTFGIPSFKLDKSLLARRREIFSAMGI
                     HFELNCEVGKDVSLDSLLEQYDAVFVGVGTYRSMKAGLPNEDAPGVYDALPFLIANTK
                     QVMGLEELPEEPFINTAGLNVVVLGGGDTAMDCVRTALRHGASNVTCAYRRDEANMPG
                     SKKEVKNAREEGANFEFNVQPVALELNEQGHVCGIRFLRTRLGEPDAQGRRRPVPVEG
                     SEFVMPADAVIMAFGFNPHGMPWLESHGVTVDKWGRIIADVESQYRYQTTNPKIFAGG
                     DAVRGADLVVTAMAEGRHAAQGIIDWLGVKSVKSH"
     gene            10787..11362
                     /gene="nudK"
                     /locus_tag="PN977_002714"
     CDS             10787..11362
                     /gene="nudK"
                     /locus_tag="PN977_002714"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose pyrophosphatase NudK"
                     /protein_id="HDI6042732.1"
                     /translation="MTQQITLIKDKILSDNYFTLHNITYDLTRKDGEVIRHKREVYDR
                     GNGATILLYNAKKKTVVLIRQFRVATWVNGNESGQLIETCAGLLDNDEPEVCIRKEAI
                     EETGYEVGEVRKLFELYMSPGGVTELIHFFIAEYSDSQRANAGGGVEDEDIEVLELPF
                     SQALEMIKTGEIRDGKTVLLLNYLQTSHLMD"
     gene            11488..12531
                     /locus_tag="PN977_002715"
     CDS             11488..12531
                     /locus_tag="PN977_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1176 domain-containing protein"
                     /protein_id="HDI6042733.1"
                     /translation="MRYRIFLLFFFALLPTSLVWAAPAQRAFSDWQVTCNNQNFCVAR
                     NTGDHNGLVMTLSRSAGAHTDAVLRIERGGLKSPDASEGEIAPRLLLDGEPLALSGDK
                     WRISPWLLVTDDTATITAFLQLIQEGKAITLRDGNQTISLNGLKAALLFIDAQQKRVG
                     SETAWIKKGDEPPLSVPPAPALKEVAVVNPTPTPLSLEERNDLLDYGNWRMNGLRCSL
                     DPLRREVNVTALTDDKALMMISCEAGAYNTIDLAWIVSRKKPLASRPVRLRLPFNNGQ
                     ETNELELMNATFDEKSRELVTLAKGRGLSDCGIQARWRFDGQRFRLVRYAAEPTCDNW
                     HGPDAWPTLWITR"
     gene            complement(12634..14637)
                     /gene="tkt"
                     /locus_tag="PN977_002716"
     CDS             complement(12634..14637)
                     /gene="tkt"
                     /locus_tag="PN977_002716"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HDI6042734.1"
                     /translation="MSRKDLANAIRALSMDAVQKANSGHPGAPMGMADIAEVLWNDFL
                     KHNPTDPTWYDRDRFILSNGHASMLLYSLLHLTGYDLPLEELKNFRQLHSKTPGHPEI
                     GYTPGVETTTGPLGQGLANAVGLAIAERTLAAQFNQPDHEIVDHFTYVFMGDGCLMEG
                     ISHEVCSLAGTLGLGKLIGFYDHNGISIDGETEGWFTDDTAKRFEAYHWHVIHEIDGH
                     DPQAVKEAILEAQSVKDKPSLIICRTVIGFGSPNKAGKEEAHGAPLGEEEVALARQKL
                     GWHHPPFEIPKEIYHAWDAREKGEKAQQSWNEKFAAYKKAHPQLAEEFTRRMSGGLPK
                     DWEKTTQKYINELQANPAKIATRKASQNTLNAYGPMLPELLGGSADLAPSNLTIWKGS
                     VSLKEDPAGNYIHYGVREFGMTAIANGIAHHGGFVPYTATFLMFVEYARNAARMAALM
                     KARQIMVYTHDSIGLGEDGPTHQAVEQLASLRLTPNFSTWRPCDQVEAAVGWKLAVER
                     HNGPTALILSRQNLAQVERTPDQVKEIARGGYVLKDSGGKPDIILIATGSEMEITLQA
                     AEKLAGEGRNVRVVSLPSTDIFDAQDEEYRESVLPSNVAARVAVEAGIADYWYKYVGL
                     KGAIVGMTGYGESAPADKLFPFFGFTAENIVAKAHKVLGVKGA"
     gene            complement(14657..15607)
                     /gene="tal"
                     /locus_tag="PN977_002717"
     CDS             complement(14657..15607)
                     /gene="tal"
                     /locus_tag="PN977_002717"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HDI6042735.1"
                     /translation="MNELDGIKQFTTVVADSGDIESIRHYHPQDATTNPSLLLKAAGL
                     SQYEHLIDDAIAWGKKNGKTQEQQVVAACDKLAVNFGAEILKIVPGRVSTEVDARLSF
                     DKEKSIEKARHLVDLYQQQGVEKSRILIKLASTWEGIRAAEELEKEGINCNLTLLFSF
                     AQARACAEAGVFLISPFVGRIYDWYQARKPMDPYVVEEDPGVKSVRNIYDYYKQHHYE
                     TIVMGASFRRTEQILALTGCDRLTIAPNLLKELQEKVSPVVRKLIPPSQTFPRPAPMS
                     EAEFRWEHNQDAMAVEKLSEGIRLFAVDQRKLEDLLAAKL"
     gene            15896..18175
                     /gene="maeB"
                     /locus_tag="PN977_002718"
     CDS             15896..18175
                     /gene="maeB"
                     /locus_tag="PN977_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxaloacetate-decarboxylating
                     malate dehydrogenase"
                     /protein_id="HDI6042736.1"
                     /translation="MDDQLKQSALDFHEFPVPGKIQVSPTKPLATQRDLALAYSPGVA
                     APCLEIEKDPLKAYKYTARGNLVAVISNGTAVLGLGNIGALAGKPVMEGKGVLFKKFA
                     GIDVFDIEVDELDPDKFIEVVAALEPTFGGINLEDIKAPECFYIEQKLRERMNIPVFH
                     DDQHGTAIISTAAILNGLRVVEKNISDVRMVVSGAGAAAIACMNLLVALGLQKHNIVV
                     CDSKGVIYQGREPNMAETKAAYAVVDDGKRTLDDVIEGADIFLGCSGPKVLTQEMVKK
                     MARAPMILALANPEPEILPPLAKEVRPDAIICTGRSDYPNQVNNVLCFPFIFRGALDV
                     GATAINEEMKLAAVRAIAELAHAEQSEVVASAYGDQDLSFGPEYIIPKPFDPRLIVKI
                     APAVAKAAMESGVATRPIADFDVYIDKLTEFVYKTNLFMKPIFSQARKAPKRVVLPEG
                     EEARVLHATQELVTLGLAKPILIGRPNVIEMRIQKLGLQIKAGVDFEIVNNESDPRFK
                     EYWTEYFQIMKRRGVTQEQAQRALISNPTVIGAIMVQRGEADAMICGTVGDYHEHFSV
                     VKNVFGYRDGVHTAGAMNALLLPSGNTFIADTYVNDEPDAEELAEITLMAAETVRRFG
                     IEPRVALLSHSNFGSSDCPSSSKMRQALELVRERAPELMIDGEMHGDAALVEAIRNDR
                     MPDSPLKGSANILVMPNMEAARISYNLLRVSSSEGVTVGPVLMGVAKPVHVLTPIASV
                     RRIVNMVALAVVEAQTQPL"
     gene            18468..18803
                     /gene="eutS"
                     /locus_tag="PN977_002719"
     CDS             18468..18803
                     /gene="eutS"
                     /locus_tag="PN977_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutS"
                     /protein_id="HDI6042737.1"
                     /translation="MDKERIIQEFVPGKQVTLAHLIAHPGEELAKKIGVPDAGAIGIM
                     TLTPGETAMIAGDLALKAADVHIGFLDRFSGALVIYGSVGAVEEALSQTVSGLGRLLN
                     YTLCEMTKS"
     gene            18816..19295
                     /gene="eutP"
                     /locus_tag="PN977_002720"
     CDS             18816..19295
                     /gene="eutP"
                     /locus_tag="PN977_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization acetate kinase EutP"
                     /protein_id="HDI6042738.1"
                     /translation="MKRIAFVGTVGAGKTTLFNALQGNYTLARKTQAVEFNDKGDIDT
                     PGEYFSHPRWYHALITTLQDVDMLIYVHGANDPESRLPAGLLDIGVSKRQIAVISKTD
                     MPDADVAATRKLLLETGFEEPIFELNSHDPQSVQQLVDYLASLTKQEEAGEKTHHSE"
     gene            19270..19971
                     /gene="eutQ"
                     /locus_tag="PN977_002721"
     CDS             19270..19971
                     /gene="eutQ"
                     /locus_tag="PN977_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization acetate kinase EutQ"
                     /protein_id="HDI6042739.1"
                     /translation="MKKLITANDIREAHARGELAMSVVLRASIITPEAREVADLLGFT
                     ITECDESTAVTTSVPASVPADKTESQRIRETIIAQLPEGQFTESLVAQLMEKVMKEKQ
                     SLEQGAMQPSFKSVTGKGGIKVIDGSSVKFGRFDGAEPHCVGLTDLVTGDDGSSMAAG
                     FMQWENAFFPWTLNYDEIDMVLEGELHVRHEGETMIAKAGDVMFIPKGSSIEFGTTSS
                     VKFLYVAWPANWQSL"
     gene            19968..20771
                     /gene="eutT"
                     /locus_tag="PN977_002722"
     CDS             19968..20771
                     /gene="eutT"
                     /locus_tag="PN977_002722"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization cob(I)yrinic acid
                     a,c-diamide adenosyltransferase EutT"
                     /protein_id="HDI6042740.1"
                     /translation="MKDFITEAWLRANHTLSEGAEIHLPADSRLTPSARELLESRHLR
                     IKFIDEQGRLFVDDEQQQPQPVHGLTSSDEHPQACCELCRQPVAKKPDTLTHLSAEKM
                     VAKSDPRLAFRAVLDSTIALAVWLQIELAEPWQPWLADIRSRLGNIMRADALGEPLAC
                     QAIVGLSDEDLHRLSHQPLRYLDHDHLVPEASHGRDAALLNLLRTKVRETETVAAQVF
                     ITRSFEVLRPDILQALNRLSSTVYVMMILSVTKQPLTVKQIQQRLGETQ"
     gene            20768..21784
                     /gene="pta"
                     /locus_tag="PN977_002723"
     CDS             20768..21784
                     /gene="pta"
                     /locus_tag="PN977_002723"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HDI6042741.1"
                     /translation="MIIERCRELALRAPARVVFPDALDQRVLKAAQYLHQQGLATPIL
                     VANPFELRQFALSHGVAMDGLQVIDPHGNLAMREEFAHRWLARAGEKTPPDALEKLTE
                     PLMFAAAMVSAGKADVCIAGNLSSTANVLRAGLRIIGLQPGCKTLSSIFLMLPQYSGP
                     ALGFADCSVVPQPTAAQLADIALASAETWRAITGEEPRVAMLSFSSNGSARHPCVANV
                     QQATEIVRERAPKLVVDGELQFDAAFVPEVAAQKAPASPLQGKANVMVFPSLEAGNIG
                     YKIAQRLGGYRAVGPLIQGLAAPMHDLSRGCSVQEIIELALVAAVPRQTEVNRESSLQ
                     TLVE"
     gene            21823..22116
                     /gene="eutM"
                     /locus_tag="PN977_002724"
     CDS             21823..22116
                     /gene="eutM"
                     /locus_tag="PN977_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutM"
                     /protein_id="HDI6042742.1"
                     /translation="MEALGMIETRGLVALIEASDAMVKAARVKLVGVKQIGGGLCTAM
                     VRGDVAACKAATDAGAAAAQRIGELVSVHVIPRPHGDLEEVFPIGLKGDSSNL"
     gene            22223..22510
                     /gene="eutN"
                     /locus_tag="PN977_002725"
     CDS             22223..22510
                     /gene="eutN"
                     /locus_tag="PN977_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutN"
                     /protein_id="HDI6042743.1"
                     /translation="MKLAVVTGQIVCTVRHHGLAHDKLLMVEMIDPQGNPDGQCAVAI
                     DNIGAGTGEWVLLVSGSSARQAHKSETSPVDLCVIGIVDEVVSGGQVIFHK"
     gene            22522..23925
                     /locus_tag="PN977_002726"
     CDS             22522..23925
                     /locus_tag="PN977_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase EutE"
                     /protein_id="HDI6042744.1"
                     /translation="MNQQDIEQVVKAVLLKMQSSDTPPAAVHEMGVFASLDDAVAAAK
                     VAQQGLKSVAMRQLAIAAIREAGEKHARDLAELAVSETGMGRVEDKFAKNVAQARGTP
                     GVECLSPQVLTGDNGLTLIENAPWGVVASVTPSTNPAATVINNAISLIAAGNSVIFAP
                     HPAAKKVSQRAITLLNQAIVAAGGPENLLVTVANPDIETAQRLFKFPGIGLLVVTGGE
                     AVVEAARKHTNKRLIAAGAGNPPVVVDETADLARAAQSIVKGASFDNNIICADEKVLI
                     VVDSVADELMRLMEGQHAVKLTAEQAQQLQSVLLKNIDECGKGTVSRDWVGRDAAKIA
                     AAIGLKVPEQTRLLFVETTAEHPFAVTELMMPVLPVVRVANVADAIALAVKLEGGCHH
                     TAAMHSRNIENMNQMANAIDTSIFVKNGPCIAGLGLGGEGWTTMTITTPTGEGVTSAR
                     TFVRLRRCVLVDAFRIV"
     gene            23936..24772
                     /gene="eutJ"
                     /locus_tag="PN977_002727"
     CDS             23936..24772
                     /gene="eutJ"
                     /locus_tag="PN977_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutJ"
                     /protein_id="HDI6042745.1"
                     /translation="MAHDEQWLTPRLQTAATLCNQTPAATESPLWLGVDLGTCDVVSM
                     VVDRDGLPVAVCLDWADVVRDGIVWDFFGAVTIVRRHLDTLEQQFGRRFSHAATSFPP
                     GTDPRISINVLESAGLEVSHVLDEPTAVADLLQLDNAGVVDIGGGTTGIAIVKKGKVT
                     YSADEATGGHHISLTLAGNRRISLEEAEQYKRGHGEEIWPAVKPVYEKMADIVARHIE
                     GQGITDLWLAGGSCMQPGVAELFRKQFPALQVHLPQHSLFMTPLAIASSGREKAEGLY
                     AK"
     gene            24762..25949
                     /gene="eutG"
                     /locus_tag="PN977_002728"
     CDS             24762..25949
                     /gene="eutG"
                     /locus_tag="PN977_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization ethanol dehydrogenase
                     EutG"
                     /protein_id="HDI6042746.1"
                     /translation="MQNELQTALFQAFDTLNLQRVKTFSVPPVTLCGPGAVSSCGQQA
                     QTRGLKHLFVMADSFLHQAGMTAGLTRSLAVKGIAMTLWPCPVGEPCITDVCAAVAQL
                     RESGCDGVIAFGGGSVLDAAKAVALLVTNPDSTLAEMSETSVLQPRLPLIAIPTTAGT
                     GSETTNVTVIIDAVSGRKQVLAHASLMPDVAILDAALTEGVPSHVTAMTGIDALTHAI
                     EAYSALNATPFTDSLAIGAIAMIGQSLPKAVGYGHDLAARESMLLASCMAGMAFSSAG
                     LGLCHAMAHQPGAALHIPHGLANAMLLPTVMEFNRMVCRDRFSQIGRALRTKKSDDRD
                     AINAVSELIAEVGIGKRLGDIGATSAHYGAWAQAALEDICLRSNPRTASLEQIIGLYA
                     AAQ"
     gene            26119..>26410
                     /gene="eutH"
                     /locus_tag="PN977_002729"
     CDS             26119..>26410
                     /gene="eutH"
                     /locus_tag="PN977_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutH"
                     /protein_id="HDI6042747.1"
                     /translation="MGINEIIMYIMMFFMLIAAVDRILSQFGGSARFLGKFGKSIEGS
                     GSQFEEGFMAMGALGLAMVGMTALAPVLAHVLGPVIIPVYEMLGANPSMFAGT"
BASE COUNT         6230 a         6843 c         7260 g         6077 t
ORIGIN      
        1 ggttgctata cggcgatggc actgttaccg atgagtgatg cgggtaaaca gctggctgaa
       61 cgtgagcatt accgtttacg tcgcgatgcg caagctctcg cgcagtggaa tggcgaaatg
      121 cttcccgttg atccactaaa cgatgccgtc ctttctgacg acgactggct tgaactggcc
      181 ggttttgctt tcgctcatcg tccgctatta acgtcgttag gttgcttaat gcgtctgtta
      241 caaaccagcg aaatggcatt accggcgctg cgtgggcgtt tacagaaaaa cgccagtgac
      301 gcgcagttat gtaccacact taaactttca ggccgtaagc tgttactggt ccgtcagcgg
      361 gaagaggccg cgcaggcgct atacgcactt gatgatgttc gcactgagcg tttgcgcgat
      421 cgcataacgc aatggcaatt ttttcactga ctccttcaat tttctcccat ggtcattgtg
      481 agtcagcggc gtaaaattaa tgacatcagt taaggagttc gccatgaaac atgaccattt
      541 tgttgttcaa agcccggata aacctgcgca acaattgtta ctgcttttcc acggtgtcgg
      601 ggataacccg gtggcaatgg gggaaatagg cagctggttt gcgccgctat ttcctgatgc
      661 gctggtggtg agtgtcggag gcgcggagcc gagcggtaat ccggcggggc gtcagtggtt
      721 ttcggtgcag ggtattacgg aagataatcg ccaggcgcgt gtggatgcca tcatgccgac
      781 gtttattgaa acggtgcgct actggcagaa acagagcgga gtaggggcta acgccacggc
      841 gctaatcggt ttctctcaag gcgcgattat ggcgctggag agcattaaag ccgaaccggg
      901 ccttgcctca cgcgtcattg cctttaacgg acggtatgcg agcctgccgg aaactgcgtc
      961 tacagccacg acaattcacc tgattcatgg cggtgaagat ccggttatcg atctggcgca
     1021 tgcggttgcg gcgcaggaag cgttaatcag tgccggtggt gacgtgacgc tggatatcgt
     1081 ggaggattta ggccatgcga tcgacaatcg cagtatgcaa tttgctctgg atcacttgcg
     1141 ttataccatt ccgaaacatt atttcgacga agcgctaagc ggcggtaagc ctggcgatga
     1201 tgatgtgatt gaaatgatgt aataatagcc gggtaggtct gataagactg tatcaggcat
     1261 catggccgga tgcggcgtta acgccttatc cggccactta gcttacttct tcggttgatc
     1321 ctttttcggc cagtcgtcgt catcgtccca cttatcgttg aaatcacgat gcgggggaag
     1381 ctccggcttg ttggcgagaa atttcttatg gtcgacgcgc ttgagatctt taatcacgtt
     1441 cagcaggacg cctaccaaaa agacaatcac cagaatccac caatatttag ccagccagtc
     1501 catgcttatt tcctcttgcg gaaccactca tcacgcgacg agctgttcca tgatacgttg
     1561 atacatacgg gcaagtagct gcaggtcggc agcgttcaca cattcattaa ttttatgaat
     1621 agtggcattg accggcccga gttccaccac ctgcgccccc atgcgggcaa taaagcgccc
     1681 gtcggacgtt ccgcctgtgg tcagtagctg cggtttaatt tcattatagt gctcaaccgc
     1741 gttaacgacc gcatccacca gtttaccgcg tgcggtcaaa aatggctgcc cggaaagcca
     1801 ccaatccacc gtatagcgca gttgatgttt ttcaagcagg gcaagtacct gcgctttgat
     1861 catctcatcc gtcagttcgg tgctgaagcg gaagttaaac tgcacaaaca gttcacccgg
     1921 aataacgttg ttactgcccg ttcccgcctg aatattggca atctgcatac tggtcgccgg
     1981 gaaaaattca ttgccctgat cccactcaat agccactaat tcattaagga aaggtgctgc
     2041 gcgatgtacc ggattgtcag ccagatgcgg gtaggcaaca tgcccctgaa cgccatgaat
     2101 ggtaaggttg caggttaatg atccgcgacg accatttttc accacatcac ctaccacttc
     2161 gatactcgac ggttcgccga ccaggcagta atcgagacgc tcattacgtg ccattaacgc
     2221 ctcgacgact tttaccgtac cgttgtgggc actggcttct tcatcagagg tgatcagaaa
     2281 tgccagtcgc cccgtatggt tgggatgttg tgcgacaaaa cgttctgccg ctaccaccat
     2341 cgccgccagc gagcctttca tatctgccgc accgcgcccg aataacatgc cgtcacgaat
     2401 ggtgggttca aacggcggat tgatccaacg gtcggcgtcg ccaggcggca ccacgtcggt
     2461 atgcccggca aaggctaacg tttcaccttg cccacgccat gcccaaaaat tctgcgtatc
     2521 ggcaaagtcc atgcgttcaa cggtaaaacc gatcgcctgc aaacgttcaa tcaacaaagc
     2581 ctggcatcct gcgtcatcag gactcaggga agggcggcga ataagctgtt gtgtcagctc
     2641 aataaccggg cacgacatag actacacctc atggaaaaat tgctgataac tggattcact
     2701 gaaacccagc agcataggct taccaggcgc gcagagcaat ggacgtttga taattgcagg
     2761 catttcagtc attaatgtcg ccgcagaggc cgcatcggtg attttattgc gggtggtttc
     2821 gtccagttta cgccaggttg taccacgggt gttgagtaac gcttcccagc ctaattcgtt
     2881 gataaaaccg ttcaaaaatt cgctgtccaa tccatcgacg cggtaatcat gaaagcgata
     2941 gtcgatgtta ttggcttcca gccaacggcg agccttttta atggtgtcac aatttttgat
     3001 gccgtaaagt gtaaccataa tgaatccttt ttcgatttat gagaataaat actcatttaa
     3061 gggtgaaaac attttgcctg caaatgatca tcaggaaata taacattatc gaagtattca
     3121 aattaacaac tattaaataa cattaaacat ttttaacgta aacattaaga atattcatct
     3181 tgttatctgt ctgtatttta accagaatta aatcagtatt agatgccgta tgaatatctc
     3241 gaaatgttgc tgtgtatcac atacaaatta tcaccgcagg accatagtat ttacataagg
     3301 tttgtccccc ggaggtagtc atcgcagcaa tcgagtaaat tagcttcacc acggtatatt
     3361 gtttccgtag aatacccata attataagag aggttgttat gattgaacgt gaactgggga
     3421 actggaaaga ctttatcgaa gttatgcttc gtaagtaatt cctgagacaa agtacaaaca
     3481 gcaagaaccc gctgagcagg ttcttaatcg gaaagttgaa aaaggcgaca cattagcatg
     3541 tcgccttttt tattgcttat tccgggcgcg gcttcagcgg gaagcggcgg cgcaccagca
     3601 caaagaacag cggcacgaag taaatagcca gaatagtggc cgaaatcatc ccgcccatta
     3661 cgccagtacc caccgcatgc tgaccaccgg aaccggcacc cgtgctggtt gccattggca
     3721 atacgccgaa gataaatgcc agcgaagtca tcagaatcgg gcgtaaacgc tgacggcagg
     3781 cgtggagcgt cgcttcaaac aggtcgtggc ctttttggtt catctcgtta gcaaactcga
     3841 cgatcaggat ggcgtttttc gccgataaac caatgaccgt taacaggccc acctggaagt
     3901 aaacgtcgtt ttccagcccg cgcatccagg ttgccagcag cgcgccgata acccccagcg
     3961 gcacgaccag cattaccgag aacggcaccg accagctttc atacaacgcc gcaagacaca
     4021 ggaataccac cagcaaggaa atggcgtaca gcgccggagc ctgcgcgccg gaaagccgct
     4081 cctgatacga catcgccgtc cactccagac caaagccgtt tggcaactgc ttcactaacg
     4141 attccataat atccatcgcc gtaccggtac tgacccccgg tgcggcttcc cctacaatct
     4201 ctaccgcaga ataaccgtta tagcgttcca gacgcggcga gcctgtctcc cagcgtgagg
     4261 tcgcgaaagc agagaagggc accatgccgc catctttatt tcggacatac cagagattga
     4321 tgtcatctgg cagcatgcga tacggcgcag ctgcctgcac atagactttc ttcacgcgac
     4381 cgcgatccat aaagtcattc acatagctcg aaccccaggc ggtttgcagt gtgtcgttaa
     4441 tatcgtcgat agcaacgccc agcgcctgag ctttacgctg gtcgatatca atctgcaact
     4501 gcggactgtc gtcgaggccg ttatggcgca cacgggttag ctccgggttt tccgccgcca
     4561 gcgccagcaa ctggttacgt gctgccatca gcgcatcgtg acccgctcca gcgtggtcct
     4621 gcaactccat atcaaaaccc gcagaactac caagaccgct aattgctggc gggctgctgg
     4681 cgataacgcg agcttcttta atcttgttaa acgccttcgt tgcacgctcg ataatggcaa
     4741 acgaggtgcc ggtcttactg tcgcgttcgc tccagtcttt caggcggata aacattctcg
     4801 ccacgttttg cccgttaccc ccagggccag aaccaacagt ggcaaacacc gacatgatgt
     4861 tgtctttttc atgggtgaag tagtatttct cgatttgctc aacgactttc agggtctgtt
     4921 gttgggttga accgctgggc aactgtaccg aggtagtaaa catgccacgg tcttccagtg
     4981 gcaagaacga cgtcggcaaa cgcaggaaca ggaacaccat gccgccaagc agcaggacat
     5041 aaatcacaat ccagcgcagg ctacggtgga gaattttcgc cacccctttt tcgtagcgtt
     5101 cggcgttgcg gttaaacatc tggttaaacc aggcaaaaaa gcctttttgc ccgtgatgct
     5161 cacctttttt cagtggctta agtagcgtgg cacacagagc cggagtgagg atcatcgcta
     5221 ccagtactga cagcaccatc gctgcaacaa tggtaataga gaactggcga tagatggcac
     5281 cggtggtgcc gccgaagaag gccattggta caaataccgc cgaaagcacc atcgcaatcc
     5341 cgaccagtgc cccctggatc tgccccatcg atttacgtgt ggcttcgcga ggagtgagtc
     5401 cttcctcact cataatacgt tcgacgtttt ccaccaccac gatggcgtca tccaccagca
     5461 gaccgatcgc cagcaccatc gcgaacatgg ttaaggtgtt gacgctgtaa ccgaaggcat
     5521 aaagtacgga gaaggttccc atcaacacca ccggcacggc gatagtgggt atcagcgtgg
     5581 cgcggaagtt ttgcaggaac agatacataa cgaggaaaac cagagcgata gcttccagca
     5641 gcgttttcac cacgtcttca atggaggctt taacaaacga ggtggtttca tacgccacct
     5701 tgtattccag tccgtgcggg aaatactgcg ccagctcgtc gagacgattg agcaccagct
     5761 ccgctgtcgc catttcgttc gcgccggagg ccagttttac ccccagcccg gaggctggca
     5821 taccattgaa gcggctaaga taatcgtatt tttccgcccc catttcgatg gtggcgacat
     5881 cgcccagcct tacctctgag ccgtcctgat tcacccgcaa ggtgatatcg cggaactgtt
     5941 ctggtgtttg tagcagtgac tgggcgttaa tggtggcgtt gagcgcctgc ttatcgacgg
     6001 aaggcgtacc accaagttgc ccaaccgcaa tctgcgcgtt ctgtgactca atggcatcag
     6061 tgacatcttt agccgtcatc tggaaactgt tgagtttcgc cgggtccagc cagatacgca
     6121 tggaatattg cgaacgatag gcatcgatat ccccgacgcc attcacgcgg cttaacggat
     6181 cctgaatatt actggcaacg taatcggcaa tatcctgttt atccatcgaa ccatcggtag
     6241 agacgaaggc aatggtcaga atattggtat cgccggtttt acgcaccgtc acgccctgat
     6301 tttgcaccgc ctgcggtaac tttcgcatgg ctgattgcag ctggttttgt acttgctgca
     6361 cggcttcgtc cggatcggtg cctgctttaa aacttaaagt gacagatgcc tggccggtgc
     6421 cactgctctg agacgacata tacatgagat tatcgaggcc ggtcatattt tgctcgataa
     6481 cctgggtcac ggtgttttcc agcgtctggg ccgatgcgcc gggatagtta gcggtcactc
     6541 gcacattcgg tggcgcgaga tcggggtatt gttcaacggg caatgaaaaa atcgccaggg
     6601 tacctgtcag acacaacagg attgccagca cccaggcaaa aatggggcga tcaataaaga
     6661 aattcgccat taaaagagga cctcgtgttt ctgcgtatcg ttgtaggctt gacttgcttc
     6721 actgtagcgg caaggtacga gccaatcgtg gagaaaaaaa ggagttaatg taaattatca
     6781 tgccctttta tactgaacct taagtgccac tattctttgg tcaataagag gcattacatc
     6841 agctgacttt cctcaccctc cgcagagcga aagctaatac tcactaacgt gccgccgccg
     6901 gaaggttgcg aaaaagtcag cgtcccaccc aatctttccg cgcgttcgcg catgatattc
     6961 agaccataat ggccttcggg ttctttcggt tcaccgatgc caatcccgtt gtcgcgaata
     7021 tagactgtgt gattgccatc cggcgcggtg acgcagctaa cggcgatttc gctggcgttg
     7081 gcgtgcttca tcgcattcag caccgcttcg cgaataattt gcaacaaatg cacctgcatt
     7141 tgcgcatcca gcgccagggt tggcagacgg cagtcgaggg tcagtttggc gctggtttga
     7201 ttttgtaacg tatccagcat ttccctcaac gcggagggga gatccgcctg ctgcagcgtc
     7261 aggcggaaag tagttaacag ctcgcgtaac tgccgataag catcattcaa cgcctgggaa
     7321 aaatcggcca tgatactttg tgcggtggca ttatcttccg gtatcgaacg cttcagtaac
     7381 gtcaactgga tacgtaagta agaaagtacc tgagccagcg agtcgtgcaa ttcgcgggcg
     7441 atggtcgcac gttcttccat caacaataac tgctgaaaat gcttctgcgc ctgattaaag
     7501 tacaaaccgc gtcccagcat cgacgaaacg ctgttaagca gcggttctga tgatgaaacg
     7561 tgactatttt gccagtgcag ttcgccgtaa accgtctctt gcattgtcac cggtaaaatc
     7621 tgcatcggca attccgggtt tggctgccct tcgctaatcc gccagttgtc accgacattt
     7681 aactccagat attcagccgc ttcattgtcg cgaacaatct gcaaaatatg gcggaaacaa
     7741 tgcacatcaa tctggctggt gtttagcgcc tgcgaacact gatacaacac ctccagacga
     7801 cgcttggcct cgtggagatc gcgggtcttt tcttctactg acgcctccag cgaacggtac
     7861 aatttatgca gctcgctcga catctggtta aaggtttttg caagcaggcc aagctcattc
     7921 ggcaggctgg tatccagcgg cggcgagtcg aactgcccgt gttcaatacg ctgactggcg
     7981 gtaaccagct gattcagcgg ggcaaccacc tgatggcgta tgcggcgcaa agtaaaaaag
     8041 accagcgtga aaataccgat gccgccagcc agggaaatcg ccaccaccag cagcattttg
     8101 cgttcagcgt agtgctgtaa agccagcacg aacaggtcta tctgattaac ataattatta
     8161 atattggcct gataccacgg caagtcgccc ttgctgagcc gattattcat ctccagccag
     8221 ttggcattca gatgcgcata gcgggttttt actgcctctg gcacatacca gacgttgaga
     8281 ttggttaata ccggtgaatg cagtgcctgc tgaaataact ggcgatgtgc attgagttgt
     8341 ggactgccac tttgcaaatc gtagcccagg cggtaactct gcatacgcag cgatccggca
     8401 atattgatag cctcagcgtc gcgcaaactg ctcgccagag ttagcagagc gatacccgtg
     8461 gaaagaatcg acagcagcac aatgtaaaaa aaggcccggg ccagactggc cgagacgggt
     8521 cgtttaacaa tcacacgcgt cttctccaca aaaaatcatt aatgttccag ttcgctgaaa
     8581 cgcaatttag tcagtgatgg gattatttga tctgccacag tttatagcga aatagttact
     8641 tattctgggc acatgaacat tgccgattcc cctgtaagtc gggtaataac aacactcata
     8701 taaagaataa ggtttttaca accaaaaaag aaggtcgtta tgaatcgttt tattatggcc
     8761 aacagtcagc aatgtctggg ttgtcatgct tgtgaaatcg cctgtgtcat ggctcacaat
     8821 gacgagcaac atgtcctgag ccaacgccat tttcatcccc gaattacggt tatcaaacat
     8881 cagcagcaac gtagtgcagt gacctgtcac cattgtgaag atgcgccctg cgcccgtagc
     8941 tgccctaatg gcgcaatcag ccacgttgat gacagcattc aggtcaatca gcaaaagtgt
     9001 attggctgta aatcctgcgt ggtggcctgt ccatttggta cgatgcaaat cgtcctgaca
     9061 cccgttgcgg ctggcaaagt taaagccacg gcgcataaat gcgacctttg tgcagggcgc
     9121 gaaaacggtc ctgcctgtgt cgaaaattgc ccggcggacg cgctgcaact ggtcactgac
     9181 gccgcactct ccggcatggc gaaatcccgc cgcttacgca ccgcacgtca ggaacatcaa
     9241 ccgtggcatg ccagtaccgc ggcacaagaa atgccggtaa tgagtaaagt cgagcaaatg
     9301 caggcaacgc ccgcgcgtgg cgagccggac aaactggcga ttgaagcgcg caaaaccggt
     9361 tttgatgaaa tttatctgcc attccgtgcc gatcaggcac aacgggaagc ctcgcgttgc
     9421 cttaagtgtg gcgagcacag cgtttgtgaa tggacctgcc cgctgcataa ccatataccg
     9481 cagtggattg aactggtgaa agcaggaaac atcgacgccg ccgtcgagct ttctcaccag
     9541 accaacaccc tgccggaaat taccggacgc gtttgcccgc aagaccgttt gtgtgaaggt
     9601 gcctgtacta ttcgcgatga gcacggcgca gtaactatcg gcaacattga acgctacatt
     9661 tcagatcagg cgttggcgaa aggttggcgt cctgacttaa gtcatgtcac caaagtggac
     9721 aagcgagtgg cgattatcgg tgcgggtccg gcagggctgg cctgtgcgga tgttctgacc
     9781 cgcaatggcg tagaggtgac ggtgtacgat cgccatccag aaatcggtgg cttgctcact
     9841 ttcggcattc cttctttcaa actggataaa tccctgctgg cacgccgtcg ggaaatcttc
     9901 agcgcgatgg ggattcactt cgaactcaat tgtgaagtgg gtaaagatgt ctctttggat
     9961 tcgcttttgg aacaatacga cgcggtcttc gttggcgtag gcacttaccg ttccatgaaa
    10021 gcgggtttac ccaatgaaga tgcgccgggc gtttatgacg ccctgccgtt cctcatcgcc
    10081 aacactaaac aggtgatggg actcgaagag ctaccggaag agccgtttat caataccgcc
    10141 ggactcaacg tcgtggtact gggcggcggc gacaccgcga tggactgtgt gcgtaccgcg
    10201 ctgcgccacg gcgcgagtaa cgtcacctgc gcttatcgtc gtgatgaagc taacatgcca
    10261 ggctcgaaga aagaggtgaa gaacgcccgc gaagaggggg ccaactttga atttaacgtc
    10321 cagccggtgg cgcttgagct gaatgagcaa ggtcacgttt gcggaattcg tttcctgcgc
    10381 acgcgtcttg gcgagccgga tgcccagggg cgtcggcgtc cagtgccggt ggaaggcagt
    10441 gaatttgtca tgccagccga cgcggtgatt atggcgtttg gcttcaatcc gcacgggatg
    10501 ccgtggctgg agtcgcacgg tgtaacggta gacaaatggg gtcgcatcat cgcggatgtg
    10561 gaaagccagt accgttacca gaccaccaat ccgaaaatct tcgctggtgg tgacgccgtg
    10621 cgtggtgcgg atctggtggt taccgcaatg gcagaaggac gtcatgcggc acaggggatt
    10681 attgactggc tgggggtaaa atcagtcaaa tctcactgat agcctgcgca gacaaacccg
    10741 acttcacagc gtaagataat tgttcatttc gcgctgtgga gtcggtatga cgcaacaaat
    10801 caccctcatt aaagacaaaa ttctctccga taactatttc accctgcaca acattactta
    10861 cgatctcacg cgcaaagatg gcgaagttat ccgtcataaa cgtgaagttt acgatcgcgg
    10921 taatggtgcg acgatcctcc tgtacaacgc gaagaaaaag accgtggttc tgattcgtca
    10981 gttccgtgtc gctacctggg ttaatggcaa tgaaagcggg cagctgattg aaacctgcgc
    11041 cgggctgctg gataacgacg aaccggaagt gtgcattcgc aaagaagcaa ttgaagagac
    11101 gggctatgaa gtgggcgaag tgcgcaaatt atttgaactg tatatgtcgc ctggcggtgt
    11161 gactgagcta atccactttt ttatcgccga atacagtgac agccagcgcg ctaacgccgg
    11221 tggtggtgtc gaagatgaag atattgaagt gcttgagctg ccgttcagcc aggcgctgga
    11281 gatgattaaa accggcgaga tacgtgacgg taagacggtg ttattgctta actatttgca
    11341 aacgtcacat ttaatggact gaaaaataac aataatattt cgttgtttat tattggctca
    11401 atccgacaaa tccgattgag ccgcgctacc tgcgcaacga agatacgact tgtgctgttt
    11461 gtttgaactt ctggggtcgt accgtccatg cgctatcgca ttttccttct cttttttttc
    11521 gctttgttgc cgacgtcttt ggtgtgggcg gcaccagcgc aacgggcgtt ttccgactgg
    11581 caggtcacct gtaataacca aaacttctgc gtggcgcgta atacgggcga tcataacgga
    11641 ctggtgatga ccctgagccg cagcgccggg gcgcataccg atgccgtttt acgtattgag
    11701 cgcggcgggt tgaagtcgcc ggatgcgtct gaaggggaga tagcgccacg gctgctgtta
    11761 gatggtgagc cgttggcgtt aagtggtgac aagtggcgga tttcaccatg gttactagta
    11821 accgatgata cggcaaccat caccgcgttt ttgcagctga ttcaggaagg gaaggcaatc
    11881 accttacgcg atggcaatca gaccatttct ctgaatggct taaaagcggc gttgttgttt
    11941 attgatgctc agcaaaagcg cgttggcagt gaaaccgcgt ggataaagaa aggggacgaa
    12001 ccgccgctca gcgtaccgcc cgcgcctgcg ctgaaagagg tcgcggtggt taacccaacg
    12061 ccgacgccac tctcactcga agaacgcaac gatttgctgg attatggaaa ctggcggatg
    12121 aatggtctgc gctgctcgct tgatccgtta cgtcgtgagg tgaatgtcac tgcgctgacc
    12181 gatgataaag cactgatgat gattagctgt gaggcagggg cctataacac cattgatttg
    12241 gcatggattg tgtcgcgtaa aaagccgcta gcctcgcgcc cggttcggtt gcgtttgccg
    12301 ttcaacaacg gtcaggagac gaatgaactg gaactgatga acgcaacatt tgatgagaaa
    12361 tcgcgtgaac tggtgacctt agcgaaaggg cgcggattaa gcgattgtgg cattcaggcg
    12421 cgctggcgct ttgacggtca gcgttttcgc ctggtgcgtt acgccgcaga acccacctgc
    12481 gataactggc atgggccaga cgcctggccc acgttgtgga tcacccgtta aacaaaatcc
    12541 cagatgcgcc cctaaaaagc gcacagactg tctcctcgcc cctctgggga gagggttagg
    12601 gttaggggaa catacagcat ccggcgatca ccatcaggca cctttcactc ccagcacctt
    12661 atgcgctttt gccacaatat tctcggcggt aaagccaaag aacgggaaca gcttatccgc
    12721 cggagcagat tccccgtaac ccgtcatccc gacaattgcc cctttcagac caacatactt
    12781 gtaccagtaa tcggcgatgc ctgcttccac cgctacgcga gccgcaacgt tagaaggcaa
    12841 caccgactcc cgatattcct catcctgggc gtcgaaaata tcggtcgagg gcagggaaac
    12901 tacgcgtaca ttgcgacctt ctcccgctaa tttctctgcc gcttgcaggg tgatttccat
    12961 ctctgaaccg gtggcaatca aaataatatc tggcttaccg ccgctgtctt tcaacacata
    13021 accaccacga gcaatctctt taacctgatc cggcgtacgt tccacctggg ccagattctg
    13081 cctggagagg atcagtgccg tcggtccgtt gtggcgctca accgccagct tccagcccac
    13141 cgctgcttcc acctgatcgc acggtcgcca ggtgctgaaa tttggcgtta agcgcagact
    13201 ggccagttgc tcaacagcct ggtgcgtagg accatcttcg cccaggccaa ttgagtcgtg
    13261 ggtataaacc ataatctgcc gcgctttcat cagtgccgcc atccgcgcgg cgttacgggc
    13321 gtattcaaca aacatcagga acgtcgcggt atacggcaca aagccgccgt ggtgcgcaat
    13381 gccgttggcg atagcggtca tgccaaattc acgcaccccg tagtgaatgt agttgcccgc
    13441 cggatcttcc ttcagcgaaa cagaaccttt ccagatggtc aggttgctgg gagccagatc
    13501 cgccgaaccg ccgagcagct ccggtagcat aggcccgtaa gcgttaagcg tattttgcga
    13561 agccttacgg gtagcgattt tcgccggatt cgcctgcaac tcattgatat atttctgagt
    13621 cgttttctcc cagtccttcg gtaaaccacc gctcatccga cgggtaaact cttctgccag
    13681 ttgcggatga gcctttttat aggcggcaaa cttctcattc cagctctgct gcgctttttc
    13741 gcctttttcg cgggcatccc aggcgtgata aatctcttta gggatctcaa atggcggatg
    13801 gtgccagccc agtttttgcc gtgccagcgc cacttcttct tcccccagtg gtgcgccgtg
    13861 cgcctcttcc ttacctgctt tattcggcga accaaagcca atcaccgtac ggcagataat
    13921 cagcgacggc ttatctttca cgctttgcgc ttcaaggatc gcttccttca ccgcctgcgg
    13981 atcgtggccg tcgatctcgt ggatcacatg ccagtgatag gcttcaaaac gttttgctgt
    14041 atcgtcggta aaccagcctt cggtttcacc gtcgatggaa ataccgttgt gatcgtaaaa
    14101 accaatcagc ttgcccagtc ccagcgtgcc cgccagcgaa cagacttcgt gggaaatacc
    14161 ttccatcagg cagccgtcgc ccataaacac ataggtgaag tgatcgacaa tttcatgatc
    14221 tggctggtta aactgcgccg ccagcgtacg ctctgctatc gccagcccga cggcgttcgc
    14281 caaaccttgt ccaagtggac cggtggtggt ttcaacacca ggcgtatagc cgatttccgg
    14341 gtgaccaggg gttttcgaat gcaactgacg aaagttcttc agttcttcca gcggcaggtc
    14401 gtaaccggtc agatgcagca aactgtagag cagcatcgac gcgtgaccgt tggaaagaat
    14461 aaagcggtcg cgatcatacc aggttgggtc ggtaggatta tgtttaagaa aatcgttcca
    14521 cagcacttcg gcaatatcag ccatgcccat tggcgcgccg ggatgaccag aattggcttt
    14581 ttgtaccgca tccatactga gtgcgcgaat cgcattggca aggtcttttc gggacatata
    14641 acactccgtg gctggtttat agtttggcgg caagaagatc ttccagtttg cgttgatcaa
    14701 cggcgaacag acgaatgcct tcagacagtt tttctaccgc catcgcatcc tgattgtgct
    14761 cccaacggaa ctccgcttcg ctcatgggag ctgggcgtgg gaacgtctga gaaggtggga
    14821 ttaatttacg taccactggc gaaacttttt cctgcagctc ctttagcaaa ttcggtgcga
    14881 tagtcagtcg atcgcagccg gttaaggcga ggatttgttc ggtgcgacgg aaactcgcgc
    14941 ccatcacaat ggtttcatag tggtgttgct tatagtagtc gtagatattg cgtactgatt
    15001 taacgcctgg atcttcttcc accacatacg ggtccatcgg cttgcgtgcc tgataccagt
    15061 cataaatacg cccgacaaac ggcgaaatca gaaatacgcc tgcttctgca caggcccgcg
    15121 cctgtgcaaa agaaaacagc agcgtcaggt tgcagttaat accttctttt tccagctctt
    15181 ctgccgcgcg aattccttcc caggttgaag ccagcttgat caaaatgcgt gatttctcaa
    15241 cgccttgttg ctgatacaag tccaccagat ggcgggcctt ttcaatactc ttttctttat
    15301 caaaagagag gcgtgcatca acttctgttg acacgcgacc gggtacgatt ttgagaattt
    15361 cagcaccgaa attgaccgcc agtttgtcac acgctgcgac cacctgttgt tcctgggttt
    15421 tgccattttt tttaccccag gcgatagcat cgtctattaa atgctcatat tgtgataatc
    15481 cggcagcctt gagtaacagc gaaggattgg tggtggcatc ctggggatga taatggcgaa
    15541 tggactcaat atcgccgctg tctgccacga cagtggtgaa ctgtttgatg ccgtctaact
    15601 cgttcatagg aaatactcct tgaaaagtaa agtgttagat gagtgcgtta attcacactt
    15661 ctgagaaatt tcgctaaacg catcaaaaaa gcatagcaga caggcatggt attgctggat
    15721 taagcaggta acatcagtgt tataggatta ttaccaaaac attatattaa tggtgagagt
    15781 ttggacttgc tcaaagtctg tagactccgg caaggtaata atgtgcgcca cgttgtgggc
    15841 aggggctgtt gcccacacac tttatttgtg aacgttacgt gaaaggaaca accaaatgga
    15901 tgaccagtta aaacaaagtg cacttgattt ccatgaattt ccagttccag ggaaaatcca
    15961 ggtttctcca accaagcctc tggcaacgca gcgcgatctg gcgctggcct actcaccagg
    16021 cgttgccgca ccttgtcttg aaatcgaaaa agacccgtta aaagcctaca aatataccgc
    16081 gcgtggtaac ctggtggcgg tgatctctaa cggtacggcg gtgctggggt taggcaacat
    16141 tggcgcgctg gcaggcaaac cggtgatgga aggcaagggc gttctgttta agaaattcgc
    16201 cgggattgat gtatttgaca ttgaagttga tgaactcgac ccggacaaat ttattgaagt
    16261 cgtcgccgcg cttgaaccga ccttcggcgg catcaacctc gaagacatta aagcgccaga
    16321 atgtttctat attgaacaga aactgcgcga gcggatgaat attccagtat tccacgacga
    16381 tcagcacggc acggcaatta tcagcactgc cgccatcctc aacggattgc gcgtggtgga
    16441 gaaaaacatc tccgacgtgc ggatggtggt ttccggcgcg ggtgcagcag caattgcctg
    16501 tatgaacctg ctggtggcgc tggggctgca aaaacataac atcgtggttt gtgattcaaa
    16561 aggcgttatc tatcagggcc gtgagccaaa tatggcggaa accaaagccg catatgcggt
    16621 ggtggatgac ggcaaacgta ccctcgatga tgtgattgaa ggtgcggata ttttcctcgg
    16681 ctgttccggc ccgaaagtgc tgacccagga aatggtgaag aaaatggctc gggcaccaat
    16741 gatcctggcg ctggcgaatc cggaaccgga aattctgccg ccgctggcga aagaagtgcg
    16801 tccggatgcc atcatctgta ccggccgttc cgactacccg aaccaggtga acaacgtcct
    16861 ttgcttcccg tttatcttcc gtggcgcgct ggacgttggc gcaaccgcca tcaacgaaga
    16921 gatgaaactg gcggcagtac gcgcgattgc agaactggcc catgcggaac agagtgaagt
    16981 ggtggcttca gcgtatggcg atcaggatct gagctttggt ccggaataca tcattccaaa
    17041 accgtttgat ccgcgcttga tcgttaagat cgctcctgcg gtggcgaaag cggcgatgga
    17101 gtcgggcgtg gcgacgcgtc cgattgctga tttcgacgtc tacatcgaca agctgacgga
    17161 gttcgtttac aaaaccaacc tgtttatgaa gccgattttc tcccaggctc gcaaagcgcc
    17221 gaagcgcgta gttctgccgg aaggggaaga ggcgcgcgtt ctgcatgcca ctcaggaatt
    17281 ggtaacgctg ggactggcga aaccgattct tattggtcgt ccgaacgtga tcgaaatgcg
    17341 cattcagaaa ctgggcttgc agatcaaggc gggcgttgat tttgagatcg tcaataacga
    17401 atccgatccg cgctttaaag agtactggac cgaatacttc cagatcatga agcgtcgcgg
    17461 tgtcactcag gagcaggcgc agcgtgcgct gatcagtaac ccgacagtga tcggcgcgat
    17521 catggttcag cgtggcgaag ccgatgcaat gatttgcggt acggtgggcg attatcatga
    17581 acactttagc gtggtgaaaa atgtctttgg ttatcgcgat ggcgttcaca ccgcaggtgc
    17641 catgaacgcg ctgctgttgc cgagtggtaa cacctttatt gccgatacct acgtcaatga
    17701 tgagcctgat gcagaagagc tggcggagat caccttgatg gcggcagaaa ctgttcgtcg
    17761 ttttggtatt gaaccgcgtg tggctctgtt gtcgcactcc aactttggtt cttctgactg
    17821 cccgtcgtcg agcaaaatgc gtcaggcgct ggaactggtc agggaacgtg cgccagaact
    17881 gatgattgat ggtgaaatgc acggcgatgc agcgctggtg gaagcgattc gcaacgaccg
    17941 tatgccggac agccctttga aaggttccgc taatattctg gtgatgccga acatggaagc
    18001 tgcccgcatt agttacaact tactgcgcgt ttccagctcg gaaggtgtga ctgtcggtcc
    18061 ggtgctgatg ggcgtggcga agccggttca cgtgttaacg ccgatcgcat cggtacgtcg
    18121 tatcgtcaac atggtggcgc tggccgtggt tgaagcgcaa acccaaccgc tgtaattctt
    18181 ttttaactct cacgcttacc ctgaatattc agggaaagcg gtttagctgc aatatattag
    18241 taaagtttat tactgagttt gcgaataata aaaaaaacag tatataaaat atctcgatat
    18301 tattttattt atattcatgc gttgcatatg aaagtttatg caccacagca aatatctctc
    18361 cattcttagt gatctacctc accttttaaa cgcgcttgcc gaattttgtt atttactctg
    18421 acgaaatatt gtcacgatac acgaaagttt ttcacaggcg gcgactcatg gataaagaac
    18481 gcatcattca ggaatttgtg cccggtaaac aggtcacact ggcgcatctc atcgcgcatc
    18541 ccggcgagga actggcgaag aaaatcggcg ttccggatgc gggcgcaatt ggcattatga
    18601 cgctaactcc cggagaaacg gcgatgatcg ccggtgattt agcgctgaaa gctgccgatg
    18661 tgcatatcgg ttttctcgac cgttttagcg gcgcgctggt gatttatggc tcggtgggtg
    18721 cagtagaaga agcgttatca caaaccgtca gtggtttagg tcgcttatta aattatacgc
    18781 tttgtgaaat gaccaaaagt taatacgagg cattcatgaa acgaattgcg tttgtgggta
    18841 ccgtcggggc aggaaaaaca acgttattta atgcgttgca ggggaattat accctcgcca
    18901 gaaaaacaca ggccgtggaa tttaatgata agggcgatat tgatacgccg ggtgaatatt
    18961 ttagccatcc ccgctggtat cacgccttaa ttaccacgct acaggatgtg gacatgctga
    19021 tctatgtcca cggcgcgaat gacccggaaa gtcggttgcc tgccgggttg ctggatattg
    19081 gcgtcagcaa acggcaaatc gccgtcatca gtaaaacgga catgccagat gctgacgtcg
    19141 ccgcaacgcg aaagttattg cttgagacgg gcttcgaaga gccgattttc gagctaaaca
    19201 gccacgaccc gcaaagcgtg cagcaactgg tggattatct ggcatctctc accaaacagg
    19261 aggaagcagg tgaaaaaact catcacagcg aatgatattc gtgaagcaca cgcgcgcggc
    19321 gaactggcaa tgtccgtggt tctgcgcgcc agcattatta ccccggaagc tcgtgaagtg
    19381 gctgatctgt tgggttttac catcaccgaa tgtgatgaat cgactgcggt aacgacgtcc
    19441 gttcccgcca gcgtgccagc cgataaaacc gaaagccagc gcatccgcga aaccatcatc
    19501 gcccagctac cggaaggcca gtttaccgaa agcctggtcg cgcagctgat ggaaaaagtg
    19561 atgaaggaaa aacagtcact ggagcagggt gcaatgcagc cgagtttcaa atcggtgacc
    19621 ggcaaaggcg gcatcaaagt gattgatggc agcagcgtca agtttggtcg tttcgatggc
    19681 gcggaaccgc actgcgtagg cttgactgat ctcgtgaccg gagacgacgg cagcagtatg
    19741 gccgctggtt tcatgcagtg ggagaacgcc ttctttccgt ggaccctgaa ctacgacgaa
    19801 atcgacatgg tgctggaagg ggaactgcat gttcgccacg aaggcgaaac tatgatcgcc
    19861 aaagcgggcg atgtgatgtt tatcccgaaa ggctccagta tcgaatttgg tacgacatcc
    19921 agcgtgaaat tcctgtatgt cgcctggccg gctaactggc aatccctatg aaagatttca
    19981 tcaccgaagc atggctaaga gcgaaccata cgctcagcga aggcgcagag atccatctcc
    20041 ctgcggacag tcgcctgacg ccctctgccc gggagttact ggaaagccgc catctgcgca
    20101 tcaagtttat tgacgagcag ggccgcctgt ttgttgacga tgaacagcag cagccgcagc
    20161 ccgttcatgg gctgaccagt agcgatgaac atccgcaggc gtgctgcgaa ctgtgccgcc
    20221 agccggtggc aaaaaagccg gatacgctga ctcacctgtc ggcggaaaaa atggttgcca
    20281 aaagcgatcc gcgcctggct tttcgcgctg ttctcgacag caccattgcg ctggcagtgt
    20341 ggctgcaaat tgaactggcg gaaccgtggc agccgtggct ggcggatatc cgttcgcgtc
    20401 ttggcaacat tatgcgtgcc gatgcgctgg gggaaccgct ggcctgccag gcgattgtcg
    20461 ggctttctga cgaagatctg catcggcttt ctcaccagcc gctgcgttat ctcgaccacg
    20521 atcatctggt gccggaagcc agccacgggc gcgatgcggc gttgctaaat ctgctacgca
    20581 ccaaagtgcg ggaaaccgaa accgtagcgg cgcaggtctt tatcacccgc agctttgaag
    20641 tattacgccc ggacatcttg caggcgctga accgcctttc cagcacggtc tacgtgatga
    20701 tgatcctgag cgtaacgaag cagccgctaa cggtgaagca gatccaacaa cgactggggg
    20761 aaacgcaatg attattgaac gttgtcgtga actggcgttg cgagcgcccg ccagagtggt
    20821 ttttccggat gcgttagatc agcgtgtgct gaaagctgcg caatatttac atcaacaagg
    20881 tctggcaacg cccattctgg tcgctaatcc gtttgaactt cgtcagtttg cgctcagtca
    20941 tggcgtagcg atggacgggc tacaggtgat tgatccgcat ggcaacctcg caatgcggga
    21001 agaatttgct catcgctggc tggcccgcgc gggcgaaaaa acgccgccgg atgcgctgga
    21061 aaaactcacc gaaccgctga tgttcgccgc cgcaatggtc agcgccggta aagcggatgt
    21121 ctgtatcgcg ggcaatctct cttccacggc gaatgtgctg cgtgccggat tacgcattat
    21181 tggcttgcag ccaggctgta aaacgctctc atccattttc ctgatgctgc cacagtacag
    21241 cggtccggcg ttgggttttg ccgattgcag cgtggtgcca cagccgacgg cggcgcagtt
    21301 ggcggatatc gcgcttgcca gcgccgaaac ctggcgcgcc atcaccggag aagaaccgcg
    21361 cgtggcgatg ctgtcgtttt ccagcaacgg tagtgcccgt cacccctgcg ttgccaacgt
    21421 gcagcaggcg acagaaatcg tccgtgagcg cgcaccaaag ctggttgtcg atggcgagtt
    21481 gcagtttgac gccgccttcg tgccggaagt ggcggcgcaa aaagcgcctg ccagcccgct
    21541 acaaggcaag gccaatgtga tggtttttcc gtcgctggaa gccggaaata ttggctacaa
    21601 aatcgcacaa cgacttggcg gatatcgtgc cgtcgggcca ttgatacaag gacttgccgc
    21661 gccgatgcac gatctctctc gtggttgtag cgtgcaggaa attatcgaac tggcgctggt
    21721 ggcagctgtg ccgcgtcaga cagaagtgaa ccgcgaaagc agtttacaaa cactggttga
    21781 atgaacggtc ccgttctgga ccctgataaa gaggaaagca caatggaagc attaggaatg
    21841 atcgaaaccc ggggcctggt tgcactgatt gaggcttctg acgcgatggt taaagctgcg
    21901 cgcgtcaagc tggttggtgt taagcagatt ggcggcggcc tgtgtactgc catggttcgt
    21961 ggtgatgttg cagcctgtaa agccgcgacc gatgcaggtg ccgcagcggc acagcgtatc
    22021 ggcgagctgg tatctgtaca cgttatcccg cgtccgcacg gcgacctgga agaagtgttc
    22081 ccgatcggcc tgaaaggcga tagcagcaac ctgtaatcac cctgtgtgac gtcgattcgg
    22141 gcgtcacgct ctctcctttt tcattttacc ttctgcggag ggtaggggga gcttttacct
    22201 gaaatacgcc acggaggcgg gtatgaaact ggcagtcgtc actggacaaa ttgtttgtac
    22261 cgtacgccat cacggactgg cgcatgacaa attgctgatg gtggaaatga ttgatccaca
    22321 aggtaatcct gacgggcaat gcgctgtcgc catcgacaat attggcgcgg gaaccgggga
    22381 gtgggtgttg ctggtgagtg gcagttccgc ccgccaggcg cataaaagcg aaacgtcacc
    22441 ggtcgatctg tgcgtgattg gcatcgtcga tgaggtggtg tctggcggtc aggtcatttt
    22501 ccacaaataa ggcagaacat catgaatcaa caggatattg aacaggtggt gaaagcggta
    22561 ctgctgaaaa tgcaaagcag tgacacgccg cccgccgccg ttcatgagat gggcgttttc
    22621 gcgtccctgg atgacgccgt tgcggcagcc aaagtcgccc agcaagggtt aaaaagcgtg
    22681 gcaatgcgcc agttagccat tgctgccatt cgtgaggcag gcgaaaaaca cgccagagat
    22741 ttagcggaac ttgccgtcag tgaaaccggc atggggcgcg ttgaagataa atttgccaaa
    22801 aacgtcgctc aggcgcgcgg cacaccaggc gttgagtgcc tctcaccaca ggtgctgacc
    22861 ggtgacaacg gcctgaccct gattgaaaac gcgccctggg gcgtggtggc ttcggtgacg
    22921 ccttccacta acccggcggc aaccgtaatt aacaacgcca tcagcctgat tgccgcgggc
    22981 aacagcgtca tttttgcccc gcatccggcg gcgaaaaaag tctcccagcg ggcgattacg
    23041 ctgctcaacc aggcgattgt tgccgcaggc gggccggaaa acttactggt tactgtggcc
    23101 aatccggata tcgaaaccgc gcaacgtttg ttcaagttcc cgggtatcgg cctgctggtg
    23161 gtgaccggcg gcgaagcggt agtggaagcg gcgcgtaaac acaccaataa acgtctgatt
    23221 gccgcaggcg ctggcaaccc gccggtagtg gtggatgaaa ccgccgacct cgcccgtgct
    23281 gctcagtcca tcgtcaaagg cgcgtctttc gataacaaca tcatttgtgc cgacgaaaag
    23341 gtactgattg ttgttgatag cgtagccgat gaactgatgc gtctgatgga aggccagcat
    23401 gcggtgaaac tgaccgcaga gcaggcgcag caactgcaat cggtgttgct gaaaaatatc
    23461 gacgagtgcg gaaaaggcac cgtcagccgt gactgggttg gtcgcgatgc ggcgaaaatc
    23521 gcggcggcaa tcggccttaa agttccggaa caaacgcgac tgctgtttgt agaaaccacc
    23581 gcagaacatc cgtttgccgt gaccgaactg atgatgccgg tgctgcccgt cgtgcgcgtc
    23641 gccaacgtgg cggatgccat tgcgctggcg gtgaaactgg aaggcggttg ccaccacacg
    23701 gcggcaatgc actcgcgcaa tatcgagaac atgaaccaga tggcgaatgc cattgatacc
    23761 agcattttcg ttaagaacgg accgtgcatt gccgggctgg ggctgggcgg ggaaggctgg
    23821 accaccatga ccatcaccac gccaaccggt gaaggggtaa ccagcgcgcg gacgtttgtc
    23881 cgtctgcgtc gctgtgtatt agtcgatgcg tttcgcattg tttaaggagt aaccgatggc
    23941 gcacgatgaa caatggctca ccccacgtct gcaaacggcg gcaacgctgt gtaaccagac
    24001 gcccgccgcg acggaatctc cgctgtggct gggggtggat ctgggcacct gcgatgtggt
    24061 gtcgatggtt gtcgaccgcg acggtctgcc ggtggcggta tgcctcgact gggccgatgt
    24121 ggtccgcgac ggcatcgtct gggatttctt cggtgcggtc accattgttc gtcgccatct
    24181 cgacaccctc gagcagcaat tcggtcgccg ttttagccat gcggcgacct cttttcctcc
    24241 aggtacggac ccgcgtattt ccatcaacgt gctggagtct gccggactgg aagtgagcca
    24301 tgtactggat gagccaaccg cagtcgcgga tctgctgcaa ctggacaacg ccggtgtagt
    24361 ggatatcggc ggtggcacta ctggtattgc catcgtgaaa aagggcaagg tgacgtactc
    24421 ggcggatgaa gcgaccggcg gacatcacat ctctctgact ctcgccggaa atcgtcgtat
    24481 ttcgctggaa gaggcagagc agtacaagcg cggtcacggt gaagagattt ggcccgcggt
    24541 gaaaccggtg tacgaaaaaa tggcggacat tgtcgcccgg catattgaag ggcagggcat
    24601 tacggattta tggctggcgg gcggctcttg tatgcaaccg ggcgtggcgg aactgtttcg
    24661 caaacaattt ccggcgttac aggtgcattt accgcagcac agtttgttta tgaccccgct
    24721 ggcgatcgcc agtagcgggc gagagaaagc ggaggggcta tatgcaaaat gaattgcaga
    24781 ccgcgctctt tcaggcgttc gataccctga atctgcaacg ggtaaaaaca tttagcgttc
    24841 caccggtgac gctttgcggt ccgggcgcgg tgagcagttg tgggcagcaa gcgcaaacgc
    24901 gtgggctgaa acatctgttc gtgatggcag acagcttttt gcatcaggca gggatgaccg
    24961 ccgggctgac acgtagcctg gccgttaaag gtatcgccat gacgctctgg ccatgtccgg
    25021 tgggcgaacc atgcatcacc gacgtgtgtg cagccgtggc gcagttgcgt gaatcaggct
    25081 gtgacggggt gattgcgttt ggcggcggct cggtgctgga tgcagcaaaa gccgtggcgt
    25141 tgctggtgac gaacccggat agcacgctgg cagagatgtc agaaaccagc gttctgcaac
    25201 cgcgcctgcc gttgattgcc attccgacta ccgccggaac cggctctgaa accaccaacg
    25261 taacggtgat tatcgatgcg gtgagcgggc gtaagcaggt gttagcccat gcctcgctga
    25321 tgccggatgt ggcgattctc gacgccgcat tgaccgaagg tgtgccgtcg catgtcacgg
    25381 cgatgactgg cattgatgcg ttaacccatg ccattgaagc gtacagcgcg ctgaacgcca
    25441 caccatttac cgacagcctg gcgattggcg ccattgcgat gattggtcaa tcgctgccga
    25501 aagcggtggg ctacggtcac gaccttgccg cgcgcgagag catgttgctg gcttcatgta
    25561 tggcgggaat ggcgttttcc agtgcggggc ttgggttgtg ccacgcgatg gcgcatcagc
    25621 caggggcggc gctgcatatt ccgcacggtc tggcgaacgc tatgttgctg ccaacggtaa
    25681 tggaatttaa ccggatggtt tgtcgtgatc ggtttagtca gattggccgg gcgctgagaa
    25741 ctaaaaaatc cgacgatcgt gacgctatta acgcggtaag tgagctgatt gcggaagtcg
    25801 ggattggtaa acgactgggc gatattggtg cgacatctgc gcattacggc gcatgggcgc
    25861 aggccgcgct ggaagatatt tgtctgcgca gcaacccgcg taccgccagc ctggagcaga
    25921 ttattggtct gtatgctgcg gcgcaataaa agtgtacgga cagtcccctc gcccctttgg
    25981 ggagagggtt agggtgaggg gaacagagtc gctccggtgc aaacaattac cctcaccccg
    26041 gtccctcggc ttccagagtg agggaaaaac ccactttcag cattggcaat aaataacaac
    26101 taacagggag taaaggcgat gggaattaac gaaatcatca tgtacatcat gatgttcttt
    26161 atgctgatag ctgccgtaga caggatcctg tcgcagttcg gcggttctgc tcgtttcctc
    26221 ggtaagttcg gtaaaagtat cgaaggatcc ggcagtcagt tcgaagaagg ctttatggca
    26281 atgggcgctt tgggcctggc gatggttggt atgaccgcgc tggcacctgt tctggcccac
    26341 gtactcggac cggtgattat cccggtttac gaaatgctcg gcgcaaaccc gtcaatgttc
    26401 gccggaacac
//
LOCUS       DAMZFT010000064        26148 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.064, whole genome shotgun sequence.
ACCESSION   DAMZFT010000064 DAMZFT010000000
VERSION     DAMZFT010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 26148)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26148)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26148
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.064"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            117..953
                     /locus_tag="PN977_002730"
     CDS             117..953
                     /locus_tag="PN977_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HDI6042748.1"
                     /translation="MLADIRYWENDATNKHYAIAHFNVWNAEMLMGVIDAAEEAKSPV
                     IISFGTGFVGNTSFEDFSHMMVSMAKKATVPVITHWDHGRSMEIIHNAWTHGMNSLMR
                     DASAFDFEENIRLTKEAVDFFHPLGIPVEAELGHVGNETVYEEALAGYHYTDPDQAAE
                     FVERTGCDSLAVAIGNQHGVYTSEPQLNFEVVKRVRDAVSVPLVLHGASGISDADIKT
                     AISLGIAKINIHTELCQAAMVAVKENQDQPFLHLEREVRKAVKERALEKIKLFGSDGK
                     AE"
     gene            958..1905
                     /locus_tag="PN977_002731"
     CDS             958..1905
                     /locus_tag="PN977_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416286.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HDI6042749.1"
                     /translation="MDNLDVICIGAAIVDIPLQPVSKNIFDVDSYPLERIAMTTGGDA
                     INEATIISRLGHRTALMSRIGKDAAGQFILDHCRKENIDIQSLKQDVSIDTSINVGLV
                     TEDGERTFVTNRNGSLWKLNIDDVDFSRFSQAKLLSLASIFNSPLLDGKALTEIFTQA
                     KARQMIICADMIKPRLNETLDDICEALSYVDYLFPNFAEAKLLTGKETLDEIADCFLA
                     CGVKTVVIKTGKDGCFIKRGDMTMKVPAVAGITAIDTIGAGDNFASGFIAALLEGKNL
                     RECARFANATAAISVLSVGATTGVKNRKLVEQLLEEYEG"
     gene            1915..2895
                     /gene="ydjG"
                     /locus_tag="PN977_002732"
     CDS             1915..2895
                     /gene="ydjG"
                     /locus_tag="PN977_002732"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent methylglyoxal reductase"
                     /protein_id="HDI6042750.1"
                     /translation="MKKIPLGTTDITLSRMGLGTWAIGGGPAWNGDLDRQICIDTILE
                     AHRCGINLIDTAPGYNFGNSEVIVGQALKKLPREQVVVETKCGIVWERKGSLFNKVGD
                     RQLYKNLSPESIREEVEASLQRLDIDYIDIYMTHWQSVPPFFTPIAETVAVLNELKAE
                     GKIRAIGAANVDADHIREYLQYGELDIIQAKYSILDRALESELLPLCRDNGIVVQVYS
                     PLEQGLLTGTITRDYVPGGARANKVWFQRENMLKVIDMLEQWQPLCARYQCTIPTLAL
                     AWILKQSDLISILSGATAPEQVRENVAALNINLSDADATLMREMAEALER"
     gene            3032..3790
                     /locus_tag="PN977_002733"
     CDS             3032..3790
                     /locus_tag="PN977_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HDI6042751.1"
                     /translation="MAAKDRIQAIKQMVANDKKVTVSNLSGIFQVTEETIRRDLEKLE
                     DEGFLTRTYGGAVLNTAMLTENIHFYKRASSFYEEKQLIARKALPFVDNKTTMAADSS
                     STVMELLKLLQDRSDLTLLTNSAEAIHVLAQSEIKVVSTGGELNKNTLSLQGRITKEI
                     ISRYHVDIMVMSCKGLDINSGALDSNEAEAEIKKTMIRQATEVALLVDHSKFDRKAFV
                     QLADFSHINYIITDKSPGAEWIAFCKDNNIQLVW"
     gene            3907..5265
                     /locus_tag="PN977_002734"
     CDS             3907..5265
                     /locus_tag="PN977_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDI6042752.1"
                     /translation="MEQYDQIGARLDRLPLARFHYRIFGIISFSLLLTGFLSYSGNVV
                     LAKLVSNGWSNNFLNAAFTSALMFGYFIGSLTGGFIGDYFGRRRAFRINLLIVGIAAT
                     GAAFVPDMYWLIFFRFLMGTGMGALIMVGYASFTEFIPATVRGKWSARLSFVGNWSPM
                     LSAAIGVVVIAFFSWRIMFLLGGIGILLAWFLSGKYFIESPRWLAGKGQIAGAESQLR
                     EVEQQIEREKSIRLPPLTLNQSNSKVKVIKGTFWLLFKGEMLRRTLVAITVLIAMNIS
                     LYTITVWIPTIFVNSGIDVDKSILMTAVIMIGAPVGIFIAALIIDHFPRRLFGSALLI
                     IIAVLGYIYSIQTTEWAILIYGLVMIFFLYMYVCFASAVYIPELWPTHLRLRGSGFVN
                     AVGRIVAVFTPYGVAALLTHYGSITVFMVLGVMLVLCALVLSIFGIETRKVSLEEISE
                     VN"
     gene            complement(5358..5999)
                     /gene="pncA"
                     /locus_tag="PN977_002735"
     CDS             complement(5358..5999)
                     /gene="pncA"
                     /locus_tag="PN977_002735"
                     /EC_number="3.5.1.-"
                     /EC_number="3.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001587135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nicotinamidase/pyrazinamidase"
                     /protein_id="HDI6042753.1"
                     /translation="MPPRALLLVDLQNDFCAGGALAVPEGDSTVDVANRLIDWCQSRG
                     EAVIASQDWHPANHGSFASQHGVEPYTPGQLDGLPQTFWPDHCVQNSEGAQLHPLLKQ
                     KAIAAVFHKGENPLVDSYSAFFDNGRRQKTALDDWLRDHEIDELIIMGLATDYCVKFT
                     VLDALQLGYKVNVITDGCRGVNIQPQDSAHAFMEMSAAGATLYTLADWEETQE"
     gene            complement(6010..7026)
                     /gene="ansA"
                     /locus_tag="PN977_002736"
     CDS             complement(6010..7026)
                     /gene="ansA"
                     /locus_tag="PN977_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HDI6042754.1"
                     /translation="MQKKSIYVAYTGGTIGMQRSEQGYIPVSGHLQRQLALMPEFHRP
                     EMPDFTIHEYTPLMDSSDMTPEDWQHIAEDIKAHYDDYDGFVILHGTDTMAYTASALS
                     FMLENLGKPVIVTGSQIPLAELRSDGQINLLNALYVAANYPINEVTLFFNNRLYRGNR
                     TTKAHADGFDAFASPNLPPLLEAGIHIRRLNTPPAPHGEGELIVHPITPQPIGVVTIY
                     PGISADVVRNFLRQPVKALILRSYGVGNAPQNKAFLQELQEASDRGIVVVNLTQCMSG
                     KVNMGGYATGNALAHAGVIGGADMTVEATLTKLHYLLSQELDTETIRKAMSQNLRGEL
                     TPDD"
     gene            complement(7193..9049)
                     /gene="sppA"
                     /locus_tag="PN977_002737"
     CDS             complement(7193..9049)
                     /gene="sppA"
                     /locus_tag="PN977_002737"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HDI6042755.1"
                     /translation="MRTLWRFIAGFFKWTWRLLNFVREMVLNLFFIFLVLVGVGIWMQ
                     VSGGDSKETASRGALLLDISGVIVDKPDSSQRFSKLSRQLLGASSDRLQENSLFDIVN
                     TIRQAKDDRNITGIVMDLKNFAGGDQPSMQYIGKALKEFRDSGKPVYAVGENYSQGQY
                     YLASFANKIWLSPQGVVDLHGFATNGLYYKSLLDKLKVSTHVFRVGTYKSAVEPFIRD
                     DMSPAAREADSRWIGELWQNYLNTVAANRQIPAQQVFPGAQGLLEGLTKTGGDTAKYA
                     LENKLVDALASSAEIEKALTKEFGWSKTDKNYRAISYYDYALKTPADTGDSIGVVFAN
                     GAIMDGEETQGNVGGDTTAAQIRDARLDPKVKAIVLRVNSPGGSVTASEVIRAELAAA
                     RAAGKPVVVSMGGMAASGGYWISTPANYIVANPSTLTGSIGIFGVITTVENSLDSIGV
                     HTDGVSTSPLADVSITRALPPEAQQMMQLSIENGYKRFITLVADARHSTPEQIDKIAQ
                     GHVWTGQDAKANGLVDSLGDFDDAVAKAAELAKVKQWHLEYYVDEPTFFDKVMDNMSG
                     SVRAMLPDAFQAMLPAPLASVASTVKSENDKLAAFNDPQNRYAFCLTCANVR"
     gene            9210..9761
                     /locus_tag="PN977_002738"
     CDS             9210..9761
                     /locus_tag="PN977_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H nitroreductase"
                     /protein_id="HDI6042756.1"
                     /translation="MDALELLINRRSASRLAEPAPTGEQLQNILRAGMRAPDHKSMQP
                     WHFFVIEGEGRERFSAVLEQGAIAAGSDNKAIDKARNAPFRAPLIITVVAKCEENHKV
                     PRWEQEMSAGCAVMAMQMAAVAQGFGGIWRSGALTESPVVREAFGCREQDKIVGFLYL
                     GTPQLKASTSINVPDPTPFVTYF"
     gene            9878..10921
                     /gene="selD"
                     /locus_tag="PN977_002739"
     CDS             9878..10921
                     /gene="selD"
                     /locus_tag="PN977_002739"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HDI6042757.1"
                     /translation="MSENSIRLTQYSHGAGCGCKISPKVLETILHSEQAKFVDPNLLV
                     GNETRDDAAVYDLGNGTSVISTTDFFMPIVDNPFDFGRIAATNAISDIFAMGGKPIMA
                     IAILGWPINKLSPEIAREVTEGGRYACRQAGIALAGGHSIDAPEPIFGLAVTGIVPTE
                     RVKKNSTAQAGCKLFLTKPLGIGVLTTAEKKSLLKPEHQGLATEVMCRMNIAGASFAN
                     IEGVKAMTDVTGFGLLGHLSEMCQGAGVQARVDYDAIPKLPGVEEYIKLGAVPGGTER
                     NFASYGHLMGEMPREVRDLLCDPQTSGGLLLAVIPEAENEVKATAAEFGIELTAIGEL
                     VPARGGRAMVEIR"
     gene            10926..12881
                     /gene="topB"
                     /locus_tag="PN977_002740"
     CDS             10926..12881
                     /gene="topB"
                     /locus_tag="PN977_002740"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016234421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HDI6042758.1"
                     /translation="MRLFIAEKPSLARAIADVLPKPHRKGDGFIECGNGQVVTWCIGH
                     LLEQAQPDAYDSRYARWNLADLPIVPEKWQLQPRPSVTKQLNVIKRFLHEASEIVHAG
                     DPDREGQLLVDEVLDYLQLAPEKRQQVQRCLINDLNPQAVERAIDRLRSNSEFVPLCV
                     SALARARADWLYGINMTRAYTILGRNAGYQGVLSVGRVQTPVLGLVVRRDEEIENFVA
                     KDFFEVKAHIVTPADERFTAIWQPSEACEPYQDEEGRLLHRPLAEHVVNRISGQPAIV
                     TSYNDKRESESAPLPFSLSALQIEAAKRFGLSAQNVLDICQKLYETHKLITYPRSDCR
                     YLPEEHFAGRHAVMNAISVHAPDLLPQPVVDPDIRNRCWDDKKVDAHHAIIPTARSSA
                     INLTENEAKVYNLIARQYLMQFCPDAVFRKCVIELDIAKGKFVAKARFLAEAGWRTLL
                     GSKERDEENDGMPLPVVAKGDELLCEKGEVVERQTQPPRHFTDATLLSAMTGIARFVQ
                     DKDLKKILRATDGLGTEATRAGIIELLFKRGFLTKKGRYIHSTDAGKALFHSLPEMAT
                     RPDMTAHWESVLTQISEKQCRYQDFMQPLVGTLYQLIDQAKRTSVRQFRGIMAPGGGE
                     GKKKDSPRKRAPKKSPSSEEAGNGVII"
     gene            complement(12958..14301)
                     /gene="gdhA"
                     /locus_tag="PN977_002741"
     CDS             complement(12958..14301)
                     /gene="gdhA"
                     /locus_tag="PN977_002741"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HDI6042759.1"
                     /translation="MDQTYSLESFLNHVQKRDPNQTEFAQAVREVMTTLWPFLEQNPK
                     YRQMSLLERLVEPERVIQFRVVWVDDRNQVQVNRAWRVQFSSAIGPYKGGMRFHPSVN
                     LSILKFLGFEQTFKNALTTLPMGGGKGGSDFDPKGKSEGEVMRFCQALMTELYRHLGA
                     DTDVPAGDIGVGGREVGFMAGMMKKLSNNTACVFTGKGLSFGGSLIRPEATGYGLVYF
                     TEAMLKRHGMGFEGMRVSVSGSGNVAQYAIEKAMEFGARVITASDSSGTVVDESGFTK
                     EKLARLIEIKASRDGRVADYAKEFGLVYLEGQQPWSVPVDIALPCATQNELDVDAAHQ
                     LIANGVKAVAEGANMPTTIEATELFQQAGVLFAPGKAANAGGVATSGLEMAQNAARLG
                     WKAEKVDARLHHIMLDIHHACVEHGGEGEQTNYVQGANIAGFVKVADAMLSQGVI"
     gene            14537..14809
                     /locus_tag="PN977_002742"
     CDS             14537..14809
                     /locus_tag="PN977_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnjH family protein"
                     /protein_id="HDI6042760.1"
                     /translation="MSRALFAVVLAFPLIALANPHYHPDVEVNVPPEVFSSGGQSAQP
                     CTQCCVYQDQNYSEGAVIKAEGILLQCQRDDKTLSTNPLVWRRVKP"
     gene            complement(14775..15182)
                     /gene="nudG"
                     /locus_tag="PN977_002743"
     CDS             complement(14775..15182)
                     /gene="nudG"
                     /locus_tag="PN977_002743"
                     /EC_number="3.6.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP pyrophosphohydrolase"
                     /protein_id="HDI6042761.1"
                     /translation="MKMIEVVAAIIERDGKILLAQRPAHSDQAGLWEFAGGKVEPDES
                     QRQALVRELREELGIEAAVGEYVASHQREVSGRIIHLHAWHVPDFHGTLQAHEHQALV
                     WCSPEEALQYPLAPADIPLLEAFMALRAARPAD"
     gene            15269..15889
                     /locus_tag="PN977_002744"
     CDS             15269..15889
                     /locus_tag="PN977_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HDI6042762.1"
                     /translation="MLDRHLHPRIKPLLHQCVRVLDKPGITPDGLTLVGFAIGVLALP
                     FLALGWYLAALVAILLNRLLDGLDGALARRRGLTDAGGFLDISLDFLFYALVPFGFIL
                     AAPEQNALAGGWLLFAFIGTGSSFLAFAALAAKHQIDNPGYAHKSFYYLGGLTEGSET
                     ILLFVLSCLFPACFPWFAWVFGALCWMTTFTRVWSGYLTLKSLQRQ"
     gene            complement(15898..17205)
                     /gene="ynjE"
                     /locus_tag="PN977_002745"
     CDS             complement(15898..17205)
                     /gene="ynjE"
                     /locus_tag="PN977_002745"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310490.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YnjE"
                     /protein_id="HDI6042763.1"
                     /translation="MKRVSQMTALAMALGLACASSWAAELAKPLTLDQLQQQNGKAID
                     TRPSAFYNGWPQTLNGPSGHEPAALNLSASWLDKMSTEQLNAWIKQHNLKADAPVALY
                     GNDKDVDAVKTRLQKAGFTHISILSDALSEPSRLQKLPHFEQLVYPQWLHDLQQGKEV
                     TAKPAGDWKVIEAAWGAPKLYLISHIPGADYIDTNEVESEPLWNKVSDEQLKAMLAKH
                     GIRHDTTVILYGRDVYAAARVAQIMLYAGVKDVRLLDGGWQTWSDAGLPVERGTPPKV
                     KTEPDFGVKIPAQPQLMLDMEQARGLLHRQDASLVSIRSWPEFIGTTSGYSYIKPKGE
                     IAGARWGHAGSDSTHMEDFHNPDGTMRSADDITAMWKAWNIRPDQQVSFYCGTGWRAS
                     ETFMYARAMGWNNVSVYDGGWYEWSSDPKNPVATGERGPDSSK"
     gene            complement(17272..17925)
                     /locus_tag="PN977_002746"
     CDS             complement(17272..17925)
                     /locus_tag="PN977_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707358.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDI6042764.1"
                     /translation="MLCVKDVSLRLPESRLLTNVNFTVDKGDIVTLMGPSGCGKSTLF
                     SWMIGALAGQFSCTGELWLNEQRIDMLPTAQRQIGILFQDALLFDQFSVGQNLLLALP
                     ATLKGNARRNAVNDALERSGLDGAFHQDPATLSGGQRARVALLRALLAQPKALLLDEP
                     FSRLDVALRDNFRHWVFSEVRAQAIPVVQVTHDLQDVPADSSVLDMAQWSENYNKLR"
     gene            complement(17925..19460)
                     /locus_tag="PN977_002747"
     CDS             complement(17925..19460)
                     /locus_tag="PN977_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HDI6042765.1"
                     /translation="MATPLRYALIFLLWAMVAVIYAPLIPATLTLISPALSLTHWQAL
                     FADPQLPQALLATLVSTTIAAVGALLIALLVIVALWPGPKWQRMCARLPWLLAIPHVA
                     FASSALLLFADGGLLYDYFPYFTPPMDKLGIGLGLTLAVKESAFLLWILAAVLSEKRL
                     LQQLIVLDSLGYSRWQCLNWLLLPSVAPALAMAMLAIVAWSLSVVDVAIILGPGNPPT
                     LAVISWQWLTQGDADQQTKGALASLLLLLLLAAYVLLGYLLWRNWRRTIPRVDGVRKP
                     ATPLLPGITLAIFLPLTGVLCVVLLAILADQSTINSEALINSLTMGLVATFIALLLLL
                     LWLEWGLQRRRLWLWLPILLPALPLVAGQYTLALWLNLDGSWTAVVWGHLLWVVPWML
                     FILQPAWQRIDSRLILIAQTLGWSRSKIFFYVKCPLMLRPTLIAFAVGFSVSIAQFMP
                     TLWLGAGRFPTLTTEAVALSSGGSNGILAAQALWQLLLPLIIFALTALVAKWVGYVRQ
                     GLR"
     gene            complement(19433..20599)
                     /locus_tag="PN977_002748"
     CDS             complement(19433..20599)
                     /locus_tag="PN977_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDI6042766.1"
                     /translation="MRHCGWLLGLLSLFSLATHASDWQEIKNEAKGQTVWFNAWGGDT
                     AINRYLDWVSGEMKTYYAINLKIVRLADAADAVKRIQTEAVAGRKTGGSVDLLWVNGE
                     NFRTLKEANLLQTGWAETLPNWRYVDTQLPVREDFSVPTEGAESPWGGAQLTFIARRD
                     VTPQPPQTPQALLEFAKANPGTVTYPRPPDFTGTAFLEQLLIMLTPDPTALKEAPDDA
                     TFARVTAPLWQYLDALHPYLWREGKDFPPSPARMDALLKANTLRLSLTFNPAHAQQKI
                     ASGDLPASSYSFGFREGMIGNVHFLTIPANANASAAAKVVANFLLSPDAQLRKADPAV
                     WGDPSVLDPQKLPDGQRETLQSRMPQDLPPVLAEPHAGWVNALEQEWLHRYGTH"
     gene            complement(20609..21157)
                     /locus_tag="PN977_002749"
     CDS             complement(20609..21157)
                     /locus_tag="PN977_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HDI6042767.1"
                     /translation="MGLPPLSKIPFILRPQAWLHRRHYGEVLSPIRWWGRIPFIFYLV
                     SMFVGWLERKRSPLDPVVRSLVSARIAQMCLCEFCVDITSMKVAERTGSTDKLLAVAD
                     WRQSPLFSDEERLALEYAEAASVTPPTVDDALRTRLATHFDAQALTELTALIGLQNLS
                     ARFNSAMDIPAQGLCRIPEKRS"
     gene            complement(21157..21864)
                     /locus_tag="PN977_002750"
     CDS             complement(21157..21864)
                     /locus_tag="PN977_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HDI6042768.1"
                     /translation="MMKMQSRKIWYYRITLIILLFAMLLAWALLPGVHEFINRSVAAF
                     AAVDQQGIERFIQSYGALAAVVSFLLMILQAIAAPLPAFLITFANASLFGAFWGGLLS
                     WTSSMAGAALCFFIARVMGREVVEKLTGKTVLDSMDGFFTRYGKHTILVCRLLPFVPF
                     DPISYAAGLTSIRFRSFFIATGLGQLPATIVYSWAGSMLTGGTFWFVTGLFILFALTV
                     VIFMAKKIWLERQKRNA"
     gene            complement(21879..22556)
                     /gene="ydjY"
                     /locus_tag="PN977_002751"
     CDS             complement(21879..22556)
                     /gene="ydjY"
                     /locus_tag="PN977_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310484.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdjY"
                     /protein_id="HDI6042769.1"
                     /translation="MLQHYSVSWKKGLTALCLLAVAGLSGCDQKENAAAKVEYDGLSN
                     SQPLRVDANNHTVTMLVQINGRFLTDDTRHGIVFKDGSNGHKSLFMGYATPKAFYEAL
                     KEAGGTPGENMTMDNKETTHVTGSKLEISVNWQGAAKAYSFDEVIVDSNGKKLDMRFG
                     GNLTAAEEKKTGCLVCLDSCPVGIVSNATYTYGAVEKRGEVKFKGNASVLPADNTLAT
                     VTFKITE"
     gene            complement(22561..23271)
                     /locus_tag="PN977_002752"
     CDS             complement(22561..23271)
                     /locus_tag="PN977_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HDI6042770.1"
                     /translation="MNAERKFLFACLIFALLIYAIHAFGLFDLLTDLPHLQTLIRQSG
                     LFGYSLYILLFIIATLFLLPGSILVIAGGIVFGPFLGTLLSLIAATLASSCSFLLARW
                     LGRDLLLKYVGHSHTFKAIEKGIARNGINFLILTRLIPLFPYNIQNYAYGLTTIAFWP
                     YTLISALTTLPGIVIYTVMASDLANEGITLRFILQLCLAGLALFILVQLAKLYARHKH
                     VDLSASRRSPLTHPKNEG"
     gene            complement(23438..24244)
                     /gene="xthA"
                     /locus_tag="PN977_002753"
     CDS             complement(23438..24244)
                     /gene="xthA"
                     /locus_tag="PN977_002753"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HDI6042771.1"
                     /translation="MKFVSFNINGLRARPHQLEAIVEKHQPDVIGLQETKVHDDMFPL
                     EEVAKLGYNVFYHGQKGHYGVALLTKETPIAVRRGFPGDDEEAQRRIIMAEIPSPLGN
                     VTVINGYFPQGESRDHPIKFPAKAQFYQNLQNYLETELKRENPVLIMGDMNISPGDLD
                     IGIGEENRKRWLRTGKCSFLPEEREWMERLMSWGLVDTFRHANPQTADRFSWFDYRSK
                     GFDDNRGLRIDLLLASQPLAECCVETGIDYEIRSMEKPSDHAPVWATFRR"
     gene            24690..25910
                     /gene="astC"
                     /locus_tag="PN977_002754"
     CDS             24690..25910
                     /gene="astC"
                     /locus_tag="PN977_002754"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.81"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylornithine/acetylornithine transaminase"
                     /protein_id="HDI6042772.1"
                     /translation="MSQPITRENFDEWMIPVYAPAPFIPVRGEGSRLWDQQGKEYIDF
                     AGGIAVNALGHAHPELREALNEQASKFWHTGNGYTNEPVLRLAKKLIDATFADRVFFC
                     NSGAEANEAALKLARKFAHDRYGSHKSGIVAFKNAFHGRTLFTVSAGGQPAYSQDFAP
                     LPPDIHHAAYNDINSASALIDDATCAVIVEPIQGEGGVVPASNAFLQGLRELCDRHNA
                     LLIFDEVQTGVGRTGELYAYMHYGVTPDLLTTAKALGGGFPVGALLATEECASVMTVG
                     THGTTYGGNPLASAVAGKVLDLINTPEMLNGVKQRHDWFVERLNSINHHYGLFSEVRG
                     LGLLIGCVLNADYAGQAKQISQEAAKAGVMVLIAGGNVVRFAPALNVSEEEVTTGLDR
                     FAAACEHFVSRGSS"
     gene            25907..>26148
                     /locus_tag="PN977_002755"
     CDS             25907..>26148
                     /locus_tag="PN977_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine N-succinyltransferase"
                     /protein_id="HDI6042773.1"
                     /translation="MMVIRPVERSDVSALMQLASKTGGGLTSLPANEATLSARIERAI
                     KTWQGELPKSEQGYVFVLEDSETGTVAGICAIEVAVG"
BASE COUNT         6461 a         6465 c         6863 g         6359 t
ORIGIN      
        1 catatttacg attatcggga tgtacaacag gcatttgaag agtcagttaa caacaaacgc
       61 gacattatta aaggcgttat taaaattagc gattaattaa tacgggagaa cagaatatgc
      121 tcgcagatat cagatattgg gaaaacgatg ccacaaataa acattatgca attgcccatt
      181 ttaacgtatg gaatgcagaa atgttgatgg gcgttatcga cgcagccgaa gaagcgaaat
      241 caccggttat tatttctttt ggtacaggtt ttgtcgggaa cacctcattt gaagatttct
      301 ctcacatgat ggtatcaatg gcaaaaaaag caacggtgcc ggtaataact cattgggatc
      361 atggtcggag tatggaaatt attcataacg cctggactca tggtatgaat tcattaatgc
      421 gtgatgcttc cgcatttgat ttcgaagaaa atattcgttt aaccaaagag gctgttgatt
      481 tcttccatcc gctgggtatt ccggtagagg cggaattagg gcatgtcggt aatgaaaccg
      541 tttatgagga agcgctggcg ggctatcact ataccgatcc tgaccaggcg gctgaatttg
      601 ttgaacgtac gggctgtgac tcattggctg tcgccatcgg caaccagcat ggggtttata
      661 cgtcagagcc acaattgaac tttgaggtcg tcaaacgcgt acgcgatgct gtttctgttc
      721 cgctggtttt gcacggcgca tcggggatca gtgatgccga cattaaaact gcaatttcgt
      781 tgggtattgc gaaaatcaac attcatacgg agctctgtca ggccgcaatg gtagcagtga
      841 aagaaaacca ggatcagcct ttcctgcatt tagaacgtga agtacgtaaa gctgtaaaag
      901 aacgggcatt ggaaaaaatt aaactgttcg gttctgatgg caaagcggaa taatgatatg
      961 gataatctcg acgttatttg tataggtgcc gctattgttg atattccatt gcaaccggtc
     1021 agtaaaaata tctttgatgt ggattcttat cctcttgaaa gaatcgcaat gaccaccggg
     1081 ggtgatgcaa taaatgaagc aacaattatt tctcgtctgg gccatcgcac agcattaatg
     1141 agtcgtattg gtaaagatgc cgccgggcaa tttattctcg accattgccg caaagagaat
     1201 attgatattc aaagcctgaa gcaggacgtc agcatagata cctcaattaa cgttggactg
     1261 gtgacggagg atggcgagcg gacgtttgtc acaaaccgta acggcagtct gtggaaattg
     1321 aatattgacg atgttgattt ttcgcggttt tctcaggcga aattattatc tctggccagt
     1381 attttcaaca gtcctctatt ggatggtaaa gcgctaacag aaatttttac gcaagctaaa
     1441 gcccggcaga tgattatctg tgccgatatg atcaaaccgc ggttgaatga aacgctggat
     1501 gatatttgcg aagcattgag ttatgtcgat tatctgtttc ctaattttgc cgaggcaaaa
     1561 ttactcaccg ggaaagagac actggatgaa attgctgact gctttcttgc gtgcggcgta
     1621 aaaacggtgg tgattaaaac gggtaaagac ggctgcttta tcaagcgtgg tgacatgacg
     1681 atgaaggtgc cggcggtcgc aggaataacc gccatcgaca ctattggcgc aggcgataac
     1741 tttgcttctg gctttatagc ggctctgtta gaaggcaaaa atctgcgtga atgcgcacgc
     1801 tttgccaatg caacggcggc tatctctgtt ttgagcgtcg gtgccaccac cggtgtaaaa
     1861 aacagaaaac tggtcgaaca attgctggaa gaatacgaag gataatgaag gcaaatgaaa
     1921 aagatacctt taggcacaac ggatattacg ctttcgcgaa tggggttggg gacatgggcc
     1981 attggcggcg gtcctgcatg gaatggcgat ctcgatcggc aaatatgtat tgatacgatt
     2041 cttgaagccc atcgttgtgg cattaatctg attgatactg cgccaggata taactttggc
     2101 aatagtgaag ttatcgtcgg tcaggcgtta aaaaaactgc cccgtgaaca ggttgtagta
     2161 gaaaccaaat gcggcattgt ctgggaacga aaagggagtt tattcaacaa agtgggcgat
     2221 cggcagttgt ataaaaacct ttccccggaa tctatccgcg aagaggtaga agccagcttg
     2281 caacgtctgg atattgatta catcgatatc tacatgacgc actggcagtc ggtgccgcca
     2341 ttttttacgc cgatagctga aactgtcgca gtgctcaatg agttaaaggc cgaaggaaaa
     2401 attcgcgcta taggcgctgc taacgtcgat gctgaccata tccgcgagta tctgcaatat
     2461 ggtgaactgg atattattca ggcgaaatac agtatcctcg accgggcatt ggaaagcgaa
     2521 ctgctgccgc tatgtcgtga taatggcatt gtggtacagg tttattcccc gctagagcag
     2581 ggattgttga ccggtaccat cacgcgtgat tacgttccgg gcggcgctcg ggcaaataaa
     2641 gtctggttcc agcgtgaaaa catgctgaaa gtgattgata tgcttgaaca gtggcagcca
     2701 ctctgtgctc gttatcagtg cacaattccc actctggcac tggcgtggat attaaaacag
     2761 agtgatttaa tctccattct tagtggggct actgcaccgg aacaggtacg tgaaaatgtc
     2821 gcggcactga atatcaactt atcggatgca gacgcaacat tgatgaggga aatggcagag
     2881 gccctggagc gttaaatact caacacgatt cacctctgtt tctgtgaaaa ctgtaatgaa
     2941 aatctgtgat tcaaacaggt tattttgaaa gtaaacatcg gttatgcgat aatcgcgcta
     3001 atgtgatgta aaaagataca ggggtgtaat cgtggcggca aaagacagga ttcaggcaat
     3061 taagcaaatg gttgccaacg ataaaaaggt gacagtctca aatttgagtg ggatttttca
     3121 ggtaaccgaa gaaaccattc gccgcgatct tgagaagctg gaagatgaag gctttttgac
     3181 cagaacctat ggtggtgctg ttttaaatac agcgatgttg acggagaata tccattttta
     3241 taagcgcgct tcatcgtttt atgaagagaa gcagcttatt gcacgcaagg cactaccctt
     3301 tgtcgacaat aaaaccacca tggcagccga ttccagtagt accgtaatgg aattgctcaa
     3361 attgttacag gaccgtagtg acctgacgtt gctaaccaac tcggcggaag cgattcatgt
     3421 tctggctcag tcagaaatta aagtcgtttc aacgggtggg gaactaaaca aaaatacact
     3481 ttccctgcaa ggaagaatta ctaaagagat catcagccgc taccatgtcg atatcatggt
     3541 aatgagttgt aaaggtcttg atattaacag cggcgcgctg gactctaacg aagcggaagc
     3601 tgaaatcaaa aagacaatga tccgtcaggc gacagaagtt gcgttattgg ttgatcactc
     3661 taagtttgat cgcaaagctt ttgtccagtt agctgacttt agtcatatta attacataat
     3721 aactgataaa tcaccgggtg cagagtggat agcattttgc aaagacaata atatccagct
     3781 ggtgtggtaa ttttagcaca cgttgtcaat attatttgtt cctgcgcctg gaaataaaat
     3841 gatgataaag atgcggcaag tgaatttgcc gcaattgcta cctttttagc aaatccgagg
     3901 cacctaatgg aacaatatga tcaaattggc gcaagactgg accgcttgcc tttggcccgg
     3961 tttcattatc gtatatttgg tattataagc tttagtctgt tattaacggg ttttttgagt
     4021 tattcaggca atgttgtttt agcaaagctg gtaagcaatg gatggtcaaa taatttcctc
     4081 aatgccgcct ttacctcggc attaatgttt ggttatttca tcggctctct taccggtggg
     4141 ttcatcggtg attattttgg gcggcgcagg gcttttcgca taaatcttct catcgtcggt
     4201 attgcggcaa caggggccgc ttttgtccct gatatgtact ggctcatctt ctttcgcttc
     4261 ctgatgggca caggaatggg ggcgctgatt atggttggct atgcctcatt tacggagttt
     4321 atccccgcga cggtgcgtgg aaaatggtcc gcgcggctct catttgttgg taactggtcg
     4381 cccatgctgt ctgcggcgat aggcgtggtg gttatcgctt tttttagttg gcggataatg
     4441 tttctgctgg gcggtattgg catactgtta gcctggtttc tctcaggtaa atactttatt
     4501 gagtcgccac gatggctggc agggaaaggg caaatcgcag gtgcagaaag ccaacttcgt
     4561 gaagtagagc agcaaattga aagagagaag agtattcgtt tacccccgct tactttgaac
     4621 cagagcaaca gcaaggttaa ggtaatcaag ggtaccttct ggctcctgtt taaaggggaa
     4681 atgttacgac gtacattagt cgcgattact gtgttaattg caatgaacat ttcgctttat
     4741 accatcaccg tatggatacc gaccatattt gttaactccg gcattgatgt tgataaatca
     4801 atattaatga ccgctgttat tatgattggc gctccggtag ggatatttat tgcggcatta
     4861 attattgatc attttcctcg tcgattattt ggctctgcct tacttattat tattgctgtg
     4921 ttaggctata tctattcaat tcaaactaca gagtgggcga ttttaatcta tggcctggtg
     4981 atgatcttct ttttatacat gtatgtgtgc ttcgcgtcgg cggtttatat cccggaactt
     5041 tggccaacgc atttacgcct gcgtggttcg ggtttcgtta atgccgtcgg acggatcgtc
     5101 gcagtcttca cgccctatgg cgttgcggca ttattaacac attatgggtc gattacggtg
     5161 tttatggtgc ttggtgtcat gttagtgctc tgtgcgctgg ttctctccat ttttggcatc
     5221 gaaacgcgga aggtgtcgct ggaagagatt tctgaggtga attaaatctt tcgttgatgg
     5281 catcaattgc cagatgtacc actgacgtgt tttatctggc ctacaatgcc agtacagaat
     5341 tgtaggccag cgtaaaatta ctcctgcgtt tcttcccagt ctgccagcgt atatagcgtt
     5401 gccccagctg ctgacatttc cataaacgcg tgcgcactgt cctgtggctg gatatttacg
     5461 ccacgacaac catcggtaat cacgttcacc ttatagccta actgtaacgc gtccagcacg
     5521 gtaaacttca cgcaatagtc agtagccagg cccataatga tcaattcatc gatttcatga
     5581 tcgcgtaacc agtcatcgag cgcagttttc tgccgacggc cgttatcaaa aaaggcactg
     5641 taactgtcaa ctaaaggatt ttcgccttta tggaacaccg ctgcgatcgc tttttgcttc
     5701 agtaacggat gtaattgtgc accttcactg ttctgcacac agtgatcggg ccagaaggtt
     5761 tgtggcaaac cgtcgagttg gcctggcgta taaggctcta caccgtgctg actggcaaaa
     5821 ctgccgtgat tcgccggatg ccagtcctga ctggcgataa ccgcttcacc gcgcgactgg
     5881 caccagtcaa tcaggcggtt agcgacatcc accgtactgt caccttccgg cacggcgagc
     5941 gcgccaccag cacagaaatc attttgtaaa tcgaccagta acagggcgcg agggggcatt
     6001 gcagtctcct taatcatccg gcgtcagttc gccacgcagg ttttggctca tggccttgcg
     6061 aatggtttca gtatccagtt cctggctaag caggtaatgc agtttggtta gtgtggcttc
     6121 tacagtcata tctgcaccgc caatgacgcc ggcatgggcg agggcgttac cggtggcata
     6181 accccccatg ttcactttac cggacataca ttgtgtcagg ttgactacca caattccgcg
     6241 atcgctggct tcttgtaatt cttgcaggaa ggctttgttt tgtggcgcat tacccacgcc
     6301 atatgagcgc agaatcaatg ctttcaccgg ttggcgcaga aaattgcgca cgacgtcagc
     6361 agaaatcccc ggataaatcg tcactacgcc aatcggttgt ggggtgattg gatgaacgat
     6421 caattcccct tcaccgtgcg gggcgggtgg cgtattcaaa cgacgaatat ggatacctgc
     6481 ttccagtaac ggaggaaggt ttggagaggc aaacgcatca aaaccatcgg catgggcttt
     6541 ggtggtacgg ttgccacgat aaagtcgatt attaaaaaag agagttactt cgttgatagg
     6601 ataattcgcc gcaacgtaca acgcattcag cagattaatt tgtccgtcag agcgtaactc
     6661 agctagcggg atttgtgacc ctgtcacaat aaccggttta ccgagattct cgagcatgaa
     6721 cgacagtgca gaggcggtat acgccatcgt gtcggtgccg tgcagaatga caaaaccatc
     6781 atagtcgtca taatgcgctt taatatcttc agcaatatgc tgccagtctt ctggcgtcat
     6841 atctgaagag tccatcagcg gcgtatattc atgaatggtg aaatctggca tctccgggcg
     6901 atggaattcc ggcatcagcg ccagttggcg ctgtagatga cctgacaccg gaatataacc
     6961 ctgctcggaa cgctgcatcc cgatggtccc gcccgtgtag gcgacgtaaa ttgatttctt
     7021 ttgcatgata ttgatatgaa tgatatcgaa agagcaaaag tatatacgtg aggcaggggg
     7081 agcgcacctg atactatgcg gataaggaaa tatggggcga gacagcggga agaggattcg
     7141 tcgacgtaaa aagtaatgtg gccgacgatc tgtcggccac ttagtacatg acttaacgca
     7201 cgttggcgca ggtcaggcaa aacgcataac ggttttgtgg gtcgttaaac gcggccagct
     7261 tgtcgttttc acttttaacg gtagaggcta ccgaggccag cggtgcaggt aacatggcct
     7321 ggaacgcatc tggcaacatt gcccggacag aaccagacat gttgtccatc actttgtcga
     7381 agaaggtcgg ttcatcaacg tagtattcca gatgccactg tttcactttt gccagctctg
     7441 ctgctttggc gaccgcatca tcgaaatccc cgagactatc gaccagcccg ttagcttttg
     7501 catcctggcc ggtccagacg tggccctggg cgattttatc aatctgctct ggcgttgaat
     7561 gacgcgcatc agcaaccagc gtgataaagc gtttatagcc attctcaatg cttaactgca
     7621 tcatctgctg tgcttccggc ggcagtgccc tggtgataga aacatccgcc agcggtgaag
     7681 ttgagacacc atcagtatga acaccaatcg aatccagact attttctacg gtggtgatca
     7741 cgccgaagat accgatagaa ccggtcaggg tgctggggtt agccacaatg taattagctg
     7801 gtgtggaaat ccagtaacca ccagatgccg ccataccgcc catcgacaca accacaggct
     7861 tacccgctgc ccgggctgct gccagttcag cgcgaatcac ttcagacgca gtaacgctgc
     7921 cgccgggact attaacacgc agaacaatcg ctttcacttt cgggtcaagg cgagcgtcgc
     7981 ggatttgtgc cgcagtggta tcaccgccaa cattcccctg agtttcctcg ccatccataa
     8041 ttgcgccatt agcgaagacg actccgatgc tgtcaccggt atctgccggc gttttcaatg
     8101 cgtaatcgta ataactgatg gcgcgataat ttttatcagt cttactccag ccgaactctt
     8161 tggtcagagc tttttcgatt tcggcactcg atgccagtgc atcgaccagc ttgttttcca
     8221 gcgcatattt cgcggtatcg ccaccggttt tggttaaacc ctcaagcaac ccttgcgcgc
     8281 cagggaatac ctgctgagca gggatctgcc ggttagcggc aacagtattc agatagtttt
     8341 gccacagctc accaatccag cggctgtcag cttcgcgggc tgccggtgac atatcatcac
     8401 gaataaacgg ttcaacggca gatttatacg tacccacgcg gaacacatgg gtggaaactt
     8461 tcagcttatc cagtaacgat ttgtagtaca gaccgttggt ggcaaaaccg tgcagatcca
     8521 ccacgccttg cggtgacagc caaattttat tggcgaaact ggcgagataa tattgcccct
     8581 ggctgtagtt ctcgccaacg gcataaaccg gtttcccgct gtcacgaaac tctttcaggg
     8641 ctttgccgat gtactgcata gacggctggt cgccgcctgc gaagtttttc agatccatca
     8701 cgataccggt gatattgcgg tcgtccttcg cctggcgaat ggtgttgacg atatcaaaca
     8761 gtgagttttc ctgcagacga tcggaactgg caccaagcag ctggcggctt aatttactaa
     8821 accgctgaga actgtcgggt ttatcgacga tcacaccaga aatgtccagc agcagtgcgc
     8881 cacgactggc cgtttctttc gaatcaccac cactgacctg catccagatc cccacaccaa
     8941 ccagcacgag aaaaataaag aacaggttaa gtaccatttc acggacgaaa ttcagcagac
     9001 gccacgtcca tttaaaaaat ccggcaataa atcgccaaag ggttcgcatg tattctccca
     9061 acttaaggtc acaccttttt caggcgcaat agatgtggtt atcgtaatga cccaaccggc
     9121 acttgtcagc aggaattgcg gactgggctg taacaaaatc cactctcgtg ttaattttgt
     9181 gaataaatat catgacagga gttaatcaaa tggatgcact cgaactattg atcaatcgcc
     9241 gtagcgcctc ccgcctggct gaacccgcgc caacgggtga acaactgcaa aacatcctgc
     9301 gtgcgggtat gcgtgcgccg gaccataagt ccatgcaacc gtggcatttc tttgtgattg
     9361 aaggggaagg gcgcgagcgt ttcagcgccg tactggagca gggggcgatt gctgccggta
     9421 gtgataacaa agccatcgac aaagcgcgta atgcgccgtt ccgcgcaccg ctcatcataa
     9481 cggtggtggc aaaatgcgaa gagaatcata aagtcccgcg ctgggaacag gaaatgtctg
     9541 cgggatgcgc ggtcatggcg atgcaaatgg cagcagttgc ccaggggttt ggcggcatct
     9601 ggcgcagtgg tgcattaact gaaagtccgg tagtgcgtga agcattcggt tgccgtgagc
     9661 aggataaaat tgtcggtttt ctctacctcg gtacgccgca gttgaaagca tctacgtcga
     9721 taaacgtccc ggacccgacg ccgtttgtga cttatttctg ataattgtcg cgaaactgtc
     9781 tggattgtga gcaaaggcgc aggaattcag acactctcac ttatcacttc atggaatgag
     9841 ggttaccata cccggattgc attgacagga gatgtccatg agcgagaact cgattcgttt
     9901 gacccaatac agccacggcg ctggttgcgg ctgtaaaatt tccccaaaag tgttggaaac
     9961 catcctgcac agtgagcagg cgaagtttgt tgatccgaat ttgcttgtgg gtaatgaaac
    10021 ccgcgacgat gcggcggtgt acgatctggg caatggcacc agcgttatca gtaccaccga
    10081 cttctttatg ccgatcgttg ataatccttt cgattttggc cgcattgcgg cgactaacgc
    10141 catcagcgat atcttcgcaa tgggcggcaa accgattatg gcgattgcga tcctcggctg
    10201 gccgattaac aaactttccc cggaaattgc ccgcgaagtg accgaaggtg gacgctatgc
    10261 atgccgtcag gcgggtattg cgctggctgg cggtcactcc atcgatgcgc cggagccgat
    10321 ttttggtctg gcggtaacgg ggatcgtacc gaccgagcgg gtgaagaaaa acagtaccgc
    10381 acaagccgga tgcaaactgt tcctgacgaa accgctgggg attggcgttc ttaccacggc
    10441 tgagaaaaaa tcactgctta aaccagaaca tcagggattg gcgacggaag tgatgtgccg
    10501 gatgaacatc gcaggcgcgt cctttgccaa catcgaaggc gtaaaagcga tgacagacgt
    10561 tacgggtttt ggtttgctgg ggcatttaag cgaaatgtgt cagggcgcag gtgtgcaggc
    10621 gcgcgtcgac tatgacgcga tcccgaaatt gcctggggtt gaagagtaca ttaagttggg
    10681 cgcagtgcct ggcggcaccg aacgtaactt tgccagctac ggtcatctga tgggtgaaat
    10741 gccgcgtgaa gtgcgcgatc tgctgtgcga tccgcaaact tctggcggtt tgctgctggc
    10801 ggtcattccg gaagcagaaa atgaggtcaa agctacagcc gccgagtttg gcattgaact
    10861 gacggcgatt ggcgaactgg tgccagcgcg cggcggtcgt gccatggttg agatccgtta
    10921 attcaatgcg gttgtttatt gccgaaaaac cgagtctggc gcgcgccatt gctgatgtcc
    10981 tgcctaaacc gcaccggaaa ggcgatggct ttatcgagtg cggtaatggt caggtggtga
    11041 cctggtgtat cggtcacctg cttgagcagg cgcagccaga cgcctacgac agccgctatg
    11101 cgcgctggaa tcttgcggat ttgccaattg tcccggaaaa gtggcaatta cagccccgac
    11161 cctccgtgac taaacaactt aatgtcatca aacgcttcct gcatgaagcc agcgaaatcg
    11221 ttcacgccgg agacccggat cgcgaagggc aactgctggt ggatgaagtg ctggactacc
    11281 tgcaactggc accggaaaag cgccagcagg tacagcgttg tttgataaac gacctgaacc
    11341 cgcaggcggt tgagcgggcg atcgatcgtc ttcgctccaa tagcgagttt gtaccgctgt
    11401 gcgtttctgc actggcgcga gcccgagctg actggctgta cggcatcaat atgacccgcg
    11461 cttacaccat tctcggtcgc aatgccggtt atcagggcgt actttccgtg ggacgcgtgc
    11521 agacgcccgt actcggactg gtggtgcgcc gcgacgaaga gattgaaaac ttcgtggcga
    11581 aagatttctt tgaagtcaaa gcacatatcg tgacacctgc cgatgagcgg tttaccgcta
    11641 tctggcaacc gagcgaagcg tgtgaaccgt accaggatga agaagggcgc ttgttacatc
    11701 gtccactggc ggagcatgtg gttaaccgca ttagtggtca accagctatt gtcaccagct
    11761 ataacgataa acgggaatca gaatccgcgc cgcttccttt ttcgctttcg gcgttgcaga
    11821 ttgaagcggc taaacgtttt ggtctgagcg cgcagaacgt gcttgatatc tgccagaagc
    11881 tgtacgaaac gcacaagcta atcacttatc cgcgttccga ttgccgctat ttgccagaag
    11941 aacattttgc cggacgccac gcggtgatga atgctatcag cgttcatgca ccggatctgt
    12001 tgccgcagcc agtggtagat ccagatatac gcaaccgctg ttgggatgac aaaaaggtcg
    12061 atgcgcacca cgccatcatt ccgaccgcac ggagttctgc gatcaacctg acggagaacg
    12121 aagcgaaggt ctataacctg attgcccgtc agtacttgat gcagttctgc ccggatgcgg
    12181 tgttccgcaa gtgtgttatc gaactggaca ttgccaaagg caaatttgtc gctaaagccc
    12241 gttttcttgc tgaagcaggc tggcgcacgc tgttaggcag caaagagcgc gatgaagaaa
    12301 acgacggcat gccgctgcct gtggtggcga aaggcgatga attgttgtgc gaaaaaggtg
    12361 aagtggtaga gcggcaaacc cagccgccgc gccattttac cgatgcaaca ctgctttcgg
    12421 cgatgaccgg gatcgcacgc tttgtgcagg acaaagatct gaaaaagatc cttcgtgcga
    12481 ccgatggtct ggggacagaa gcaacccgtg ccgggattat tgaactgttg ttcaagcgtg
    12541 gtttcctgac caaaaaaggg cgctatatcc actccaccga cgccggaaaa gcgctattcc
    12601 attcgctgcc agaaatggcg acgcgaccgg acatgaccgc gcactgggaa tcggtgctga
    12661 cgcaaatcag tgaaaagcag tgtcgctatc aggactttat gcagccgctg gtggggacat
    12721 tgtaccagtt gattgatcag gcaaagcgca cgtcggtaag gcagtttcgg ggaataatgg
    12781 ctcccggcgg tggagaaggg aagaaaaagg attcgccacg caagagagcg ccgaaaaaaa
    12841 gcccgtcatc agaagaggcg ggcaatggag tgataatata aaggttcttt caaccatctg
    12901 atactgaaga tttttatggc cggataaggc gtttgcgctg tatccggcac aattgtctta
    12961 aatcacgccc tggcttaaca tcgcatccgc aaccttcaca aaaccggcaa tgttcgcgcc
    13021 ctgcacgtag ttggtttgct caccttcacc accatgctca acacaggcat ggtggatatc
    13081 cagcatgatg tgatgcaaac gtgcgtcaac tttctcggct ttccagccca ggcgcgcagc
    13141 gttttgcgcc atttccaggc ccgatgttgc tacaccgcca gcattagccg ctttacccgg
    13201 tgcaaacagt acgcctgcct gttggaacag ttcagtcgct tcgatggtgg tcggcatatt
    13261 tgccccttcg gcgacggctt taacgccatt ggcgataagc tgatgcgcgg cgtcaacatc
    13321 cagttcgttc tgggtggcgc aaggcagggc gatatcaacc ggtacagacc acggttgttg
    13381 gccttcgaga tagaccagac caaattcttt ggcgtaatct gccactcgac catcgcggct
    13441 ggctttgatt tcgataagac gtgccagttt ctctttcgtg aatccgcttt catcaactac
    13501 agtgccactg gagtctgacg cagtgatcac acgagcacca aattccatcg ctttttcgat
    13561 agcgtactgg gcgacgttgc cggagccaga aacggaaacg cgcattcctt caaaacccat
    13621 accgtggcgt tttagcattg cttctgtgaa ataaaccaga ccgtatccgg tagcttccgg
    13681 gcgaataaga ctgccgccaa atgaaaggcc cttaccggtg aagacgcagg cggtattgtt
    13741 ggagagcttt ttcatcatcc ccgccataaa gccgacttca cgaccaccaa ccccgatatc
    13801 acctgccgga acgtcggtat ccgcgcccag gtggcgatac agttcagtca tcagcgcctg
    13861 gcaaaaacgc atcacttcac cttcgctttt tcctttcgga tcgaaatcgc tgccgccttt
    13921 accaccgccc atcggcagag tagtcagggc atttttgaag gtttgttcga agccgaggaa
    13981 tttgagaatg gaaaggttaa ctgacggatg gaagcgcata ccgcctttgt acgggccgat
    14041 ggcagaactg aactgcacac gccatgcacg gttgacctgt acctggttgc gatcatcaac
    14101 ccataccacg cgaaactgga tcacgcgctc cggttcaacc agacgctcca gtaatgacat
    14161 ctggcgatat tttggatttt gttcaagaaa aggccagagt gtggtcatta cttcacgaac
    14221 ggcttgcgcg aactcggttt gattcgggtc gcgcttttgg acatggttga ggaatgactc
    14281 cagagaatat gtctgatcca tagatataaa acccttatat attaatacga ttgtgcttat
    14341 gttgtttatg tcatgtgctt ttgcagtttt cgactatacc atcaagaatg tgacggtaag
    14401 caagaaaaaa atgacccagg aaagcaaatt aataacgaga gtaatctcat aaccaaatgc
    14461 gataactcag acgctaaagt tttgccaccc ggctaccgtt atcagtagca gagacaataa
    14521 cgttaaggag ttccctgtga gtcgagcatt gttcgccgta gtattagcat ttccattaat
    14581 tgcactggct aacccgcatt atcatccgga cgtggaggta aacgtaccgc cagaagtgtt
    14641 cagttcaggc gggcagtcag cgcagccctg tactcagtgc tgcgtttatc aggatcaaaa
    14701 ttattctgaa ggggcggtaa ttaaagccga aggaatactg ctgcaatgcc agcgtgatga
    14761 caaaaccctt agcactaatc cgctggtctg gcggcgcgta aagccataaa cgcctctaat
    14821 agtggaatgt cagcaggggc cagcggatat tgcagcgcct cttcaggtga gcaccagacc
    14881 agcgcctgat gttcatgtgc ctgtaacgtc ccgtggaagt cgggtacgtg ccaggcatga
    14941 agatggataa tccgccccga aacttctcgc tgatggctgg caacatattc acccacagct
    15001 gcttcgatgc ccagttcttc gcgtaactca cgcaccagcg cctgccgctg gctttcatcc
    15061 ggctcgactt taccaccggc aaactcccat aatcccgcct gatcgctatg ggcggggcgt
    15121 tgcgcgagta aaattttgcc atcacgttcg atgatggcgg caacaacttc aatcattttc
    15181 ataaattaca cacgggcaga taagcgaaaa atgccataat atccgcactg ttgctcaatc
    15241 agaaaggctt tatatttaag gagtgcctgt gctagaccgc catcttcatc cccggattaa
    15301 accgttgttg catcagtgcg tgcgggttct tgataaaccg ggcattacgc cagatggttt
    15361 aacattagtg ggatttgcca tcggcgtgct ggcactgcct tttctggcgc tgggctggta
    15421 tctggcggcg ctagtcgcca ttttgttgaa caggctgctt gatggtctgg acggtgcgct
    15481 ggcgcggcgc agagggctta ccgacgcggg cggctttctc gatatttctc tcgattttct
    15541 cttttacgcg ctggtgccgt ttggttttat tcttgctgca ccggagcaaa atgcgctggc
    15601 aggcggctgg ctgctgtttg cgtttatcgg cacgggcagc agttttctcg cttttgctgc
    15661 gctggcggcg aaacatcaga ttgataatcc tggttatgcg cataaatcgt tttattatct
    15721 gggcgggtta accgaaggca gcgagacgat tttgctgttt gttctgagtt gcctctttcc
    15781 ggcctgtttt ccgtggtttg cgtgggtatt tggtgcgttg tgctggatga cgacctttac
    15841 gcgggtgtgg agtgggtatc tgacgctgaa gtcgctccag cgtcagtaat tgcaatgtta
    15901 tttgctgctg tccgggccgc gttcaccggt tgctaccgga ttttttggat cgctgctcca
    15961 ttcgtaccag ccgccgtcgt aaacggagac gttattccag cccattgctc gtgcgtacat
    16021 aaaggtttcg gacgcgcgcc agccggtgcc gcagtagaat gaaacttgct gatctggtct
    16081 gatattccat gctttccaca tagccgtaat atcatcggcg ctgcgcatgg tgccatccgg
    16141 gttatggaaa tcttccatat gcgtcgagtc gctaccagcg tgtccccaac gtgctccggc
    16201 tatttcacct tttggtttaa tatagctgta accactggtc gtaccgataa actccggcca
    16261 cgaacgaata ctcaccagcg aggcgtcctg gcgatgcagc agtccacgcg cctgttccat
    16321 atcaagcatc agttgcggtt gtgccgggat cttcacgccg aaatccggtt ccgttttcac
    16381 tttcggtggc gttccgcgct caacgggcag tcccgcgtcg gaccaggttt gccagccgcc
    16441 gtccagcagg cggacatctt tcacgccagc gtagagcata atttgtgcca cacgcgctgc
    16501 agcgtatacg tcacgcccgt acagaatgac cgtggtgtca tggcgaatgc cgtgttttgc
    16561 cagcatcgct ttcagttgtt catcagaaac tttgttccac agcggttcgc tttccacttc
    16621 gttggtatcg atgtagtcag cgccgggaat atggctgata aggtaaagct taggagcgcc
    16681 ccaggccgct tcaatgactt tccagtcacc ggcaggtttc gccgtaacct ctttaccttg
    16741 ttgcaggtcg tgcagccatt gcggataaac cagctgctca aaatgcggca gtttttgcag
    16801 acgggaaggt tcgcttagcg cgtcactcag aatggagata tgcgtaaaac ctgctttttg
    16861 cagtcgcgtt ttgacggcgt cgacatcttt gtcattaccg tacagcgcca ccggagcatc
    16921 ggctttcagg ttatgttgct tgatccacgc gttgagctgt tcggtgctca ttttgtcgag
    16981 ccagctggca gagaggttta aggcggcagg ttcatgacca gaagggccat ttaaggtttg
    17041 tggccagccg ttataaaacg cgctggggcg agtatctatc gctttgccat tttgttgttg
    17101 aagctggtca agtgtaagag gcttcgccag ttcagcggcc cacgaagaag cgcaagccag
    17161 ccctaaagcc attgccagcg cggtcatttg agaaacacgt ttcatcgaaa atcccgacat
    17221 tgtagttttg agtgcgccat tctgaactga cgcattgaga aaaactttgc gttatcgcag
    17281 tttgttgtaa ttttctgacc actgcgccat atccagaaca gaactatcag caggaacatc
    17341 ctggagatcg tgcgttacct gaacgacggg gatcgcctgg gcgcgaactt cgctgaacac
    17401 ccaatggcga aaattatcgc gcagggccac atcaagacgg ctgaatggct catccagtag
    17461 caacgctttt ggctgagcga gaagggcgcg tagcagagca acgcgcgctc gctgaccgcc
    17521 agacaaagtg gcaggatcct gatggaaagc tccgtcaagg cctgaacgct caagtgcatc
    17581 attcacggca ttacgtcggg cattaccctt aagtgtcgcc ggtagcgcca gcagtaaatt
    17641 ttgcccgacg ctgaactgat caaataacag tgcatcctgg aaaagaatgc caatctgacg
    17701 ctgtgcggtg ggtagcatgt caatccgttg ctcattaagc catagctccc ctgtacaaga
    17761 aaactgtccg gccagcgcac caatcatcca tgaaaacaga gtggattttc cacagccaga
    17821 cggccccatt aacgtgacaa tgtcaccttt atccaccgta aagttaacgt ttgtcagcaa
    17881 gcggctttct ggtaaacgta gcgaaacatc tttcacgcag agcattagcg gagtccttgt
    17941 ctgacataac ctacccattt tgcgactaac gcggtcaggg caaaaataat aagcggtaat
    18001 agcagttgcc ataaagcctg ggcggcaaga ataccgttgc tgccgccgct gcttaatgcc
    18061 accgcctcag tggtgagcgt cggaaaacgc cccgcgccca gccacagcgt cggcataaac
    18121 tgcgcaatac tgactgaaaa tcccaccgcg aaggcaatca gcgtagggcg tagcataagc
    18181 ggacatttca cgtaaaagaa gattttggac cgcgaccagc ccagtgtttg cgcaatcaaa
    18241 attaaccgtg aatcaatgcg ctgccaggcg ggttgcagga taaacagcat ccacggcact
    18301 acccacagca gatgccccca gaccaccgcc gtccagcttc catccaggtt cagccatagc
    18361 gccagcgtgt actggcccgc caccagtggc agagcgggta ataaaatggg caaccatagc
    18421 cacaaccggc ggcgttgtag cccccattcc agccacagca gtaacaggag caaagcgatg
    18481 aatgtcgcca ccagccccat tgtcaggctg ttgatgagcg cttcactatt gatcgtcgac
    18541 tgatccgcga ggatcgccag cagaaccaca cacagcacac cggtcaaggg taaaaaaatc
    18601 gccagcgtaa tgcctggtag taaaggtgtg gcaggcttac gaacgccatc tacgcgggga
    18661 atagtacgcc gccagttacg ccatagcaga tagcccagca aaacgtaggc ggcgagtaac
    18721 agcagcagca gcaggctggc gagtgcgcct ttcgtttgtt gatcggcgtc gccctgagtt
    18781 aaccactgcc agctaattac cgccagcgtc gggggattac ctggcccaag aataatcgcc
    18841 acatccacga ccgacagcga ccaggcgaca atcgccagca tcgccattgc cagtgcaggt
    18901 gcgacggagg gcaacaacag ccagttcagg cattgccagc ggctgtagcc cagcgaatcc
    18961 agcacaatga gttgctgcaa cagccgtttt tcgctcaata ctgccgctaa gatccacagc
    19021 agaaatgcgc tttctttcac cgccagggtg aggcccagcc caatacccaa tttgtccatt
    19081 ggcggagtga aatatgggaa atagtcataa agcagccctc cgtcagcaaa gagcagaagg
    19141 gcgctggagg caaaagccac atggggaatg gcgagcagcc acggcagacg ggcgcacata
    19201 cgctgccatt tcggcccagg ccacagcgcc acaatcacca gcaaggcaat caacaacgcc
    19261 ccgaccgccg cgatggttgt cgacaccagc gttgccagta atgcctgcgg taactgtgga
    19321 tcggcaaata acgcctgcca gtgtgtcaac gacaaagcag gcgatatcag cgtgagagtt
    19381 gccgggatca gcggtgcata aatcaccgcc accatcgccc acagcaaaaa gattaatgcg
    19441 taccgtaacg gtgtagccat tcttgttcca gcgcatttac ccaacctgcg tgcggttcag
    19501 ccagtaccgg cggcagatcc tgcggcattc tcgattgcaa tgtttcgcgc tgcccgtcag
    19561 gcagtttttg cggatcgaga acagaaggat cgccccagac agcgggatct gctttacgca
    19621 gttgcgcatc gggtgagagc aagaaattgg caactacctt cgccgcagca ctggcattcg
    19681 cgttggcagg aatggtgagg aaatggacgt tgccaatcat cccctcgcga aaaccaaaac
    19741 tgtaactgct tgcaggcaaa tcgccgctgg cgattttttg ctgcgcatgt gcagggttaa
    19801 aggtcagcga caggcgcaat gtgttagctt tcagcagagc atccatccgc gcgggcgaag
    19861 gcgggaaatc ctttccttcg cgccacaaat acggatgcag tgcatcaaga tattgccaca
    19921 agggcgcagt gacgcgggcg aaagtcgcat cgtccggcgc ttcttttaat gctgtgggat
    19981 ctggcgttag cataatcagc aactgttcaa gaaacgccgt gccggtaaag tccggtgggc
    20041 gcggataggt aaccgtgccg ggattggctt tagcaaactc cagtaaggct tgcggcgttt
    20101 gtggtggctg cggcgtaaca tcgcggcggg cgataaacgt cagttgcgcg cccccccagg
    20161 gcgattcagc cccttctgta ggcactgaaa aatcttcccg caccggcagc tgtgtgtcga
    20221 catagcgcca gttgggcaga gtctccgccc agcccgtttg taataaattg gcctctttta
    20281 aggtgcggaa gttttcgccg ttcacccaga gcagatccac cgagccgccc gttttacgtc
    20341 cggcaacagc ttcggtctga atgcgcttta ccgcgtctgc ggcatccgcc agacgaacaa
    20401 tcttcaggtt tatagcgtaa taggttttca tctcgccgct aacccagtcg agatagcggt
    20461 taattgcggt atcgccgccc caggcgttaa accagacggt ttgtcctttg gcctcatttt
    20521 ttatttcctg ccagtcactg gcatgtgttg ccaaagaaaa cagcgataac aatcccagca
    20581 accacccaca atggcgcatc atctctcctt aagaacgttt ttcaggaata cggcacagcc
    20641 cctgagcggg aatgtccatg gcagaattaa aacgggctga cagattttgc aggccgatca
    20701 atgccgtcag ttcggtgagt gcctgagcgt caaaatgggt agccagtcgg gtacgcaggg
    20761 catcatcgac cgttggcggt gtgacgcttg cggcttcggc gtactccagc gccagccgtt
    20821 cttcatcgct aaagagcggg ctttgccgcc agtcagccac tgccagcagt ttatcggtgc
    20881 tgccggtacg ctcggcgact ttcatactgg tgatatccac gcaaaactca cacaggcaca
    20941 tttgcgcaat gcgcgcgctg acaagcgatc gcactaccgg atcgagcggt gagcgtttgc
    21001 gctccagcca gccgacaaac atcgacacca gataaaagat aaacgggatc cgcccccacc
    21061 agcgaatggg gcttagcacc tcgccgtaat ggcgacgatg cagccacgcc tgcggacgta
    21121 aaatgaaagg aattttgcta agcggcggta aacccatcag gcattcctct tctggcgctc
    21181 aagccatatt ttcttcgcca taaaaatcac cacggtcagg gcaaacagaa taaacagtcc
    21241 ggtgacaaac cagaaagtac cgcctgttaa catgctgccc gcccaggaat aaacaatagt
    21301 tgccggtaac tgaccaagcc cggtggcgat aaaaaacgag cgaaaacgta ttgaagtcaa
    21361 accggcagca tagctgattg gatcgaaagg gacaaaaggc aataaccgac ataccaggat
    21421 ggtgtgtttg ccgtagcgag tgaaaaagcc gtccatactg tcaagtacgg ttttgccggt
    21481 taatttttcc accacttcgc ggcccatcac tctggcgata aaaaagcaca gcgccgcgcc
    21541 ggccatcgaa ctggtccacg acagtaagcc gccccagaac gcgccaaaca gcgacgcatt
    21601 ggcaaaggtg atcaaaaacg caggtagcgg tgcagcaatg gcctgtaaaa tcatcaacaa
    21661 gaacgagaca actgccgcta gtgcgccgta agactgaata aagcgttcta taccctgttg
    21721 gtccacggcg gcaaacgccg caacgctgcg attgataaac tcatggacgc cgggaagcag
    21781 cgcccatgcc agcagcatag cgaacaacag gatgatgagg gttatacggt agtaccagat
    21841 ttttcgcgat tgcatcttca tcatccttta tcctggcttt attcggtgat tttaaaggta
    21901 accgttgcca gcgtgttatc cgccgggaga accgaggcat tgcctttaaa tttaacttca
    21961 ccgcgttttt caaccgcacc ataagtgtat gttgcattgc tgacgatgcc gaccgggcag
    22021 ctatccagac acaccaggca gcccgttttc ttctcttctg ctgccgttaa attaccgcca
    22081 aagcgcatgt ccagtttctt gccattactg tcaacaatca cttcatcgaa ggaatacgct
    22141 tttgccgccc cttgccagtt gaccgaaatt tccagtttgc tgcctgtgac atgagtcgtt
    22201 tctttattat ccatcgtcat gttttcgccc ggtgtaccac ctgcctcttt cagggcttca
    22261 taaaatgctt tcggggtcgc ataacccata aacagcgatt tatgtccgtt ggagccatct
    22321 ttaaacacaa taccgtgacg agtgtcgtcg gtgaggaaac gaccattaat ttgcaccagc
    22381 atggttaccg tatggttatt ggcatcgaca cgcagcggtt ggctgttcga aagtccgtcg
    22441 tattccactt ttgccgccgc attctctttt tgatcgcagc cgctaagccc agcaacagcc
    22501 agtaagcaga gtgcagtcag tccttttttc catgacactg aataatgttg caacatcgtt
    22561 ctatccttca ttttttgggt gagtaagtgg gctgcggcga gaagcagaca gatccacatg
    22621 tttgtgtcgg gcgtagagtt ttgcgagctg gacgagaata aacagcgcca ggcccgccag
    22681 acagagttgt aaaataaagc gcagcgtaat gccttcattg gcgagatcgc ttgccatcac
    22741 ggtataaata acaataccgg gcagagtcgt gagtgctgaa ataagggtat aaggccagaa
    22801 ggcgattgtg gttaatccgt aagcgtaatt ttgaatattg taaggaaaca acgggattaa
    22861 gcgggtcaga ataagaaaat taataccgtt acgcgcaatg cctttttcaa tggccttaaa
    22921 agtatggcta tggcccacat atttcagcag taaatcacgc cccagccagc gcgccagcag
    22981 gaatgagcac gaggaggcca gcgtggcggc aattaatgag agtagtgtcc ctaagaacgg
    23041 gccaaaaact attcctccag cgatcaccag tatgcttcct ggtaacagaa agagggtggc
    23101 aatgatgaat aacaaaatat agaggctata accgaacaat ccgctctggc ggatgagtgt
    23161 ttgtaagtga ggtaaatcgg tgagcagatc gaataaaccg aaagcgtgga tagcgtaaat
    23221 gagcagcgca aaaataagac aggcaaaaag aaatttacgc tcagcgttca tcgtggagtt
    23281 tcctatatca aacttgataa tagagaagtg gtcagacaac taaaaacgca gtgacggtat
    23341 aacaaagggc ggcaggcaac aaatcattac tgacagtttg ttgatttttc tcaaggttaa
    23401 ttctcctgac ccagtttgag ccaggagaac agctaaatta gcggcggaag gtcgcccaga
    23461 cgggggcgtg atcggacggt ttttccatgc tgcggatttc atagtcgatg ccggtttcta
    23521 cgcaacattc tgccagcggt tggctggcga gaagcaggtc gatacgcaga ccgcgattat
    23581 cgtcaaaacc ttttgagcgg taatcaaacc atgagaaacg atcggctgtt tgcggattcg
    23641 catggcggaa ggtatcgacc aacccccagc tcatcagtct ctccatccat tcgcgttctt
    23701 ccggaaggaa agagcatttt ccggtacgca gccagcgctt acggttctct tcgccaatgc
    23761 caatatccag gtcgccagga ctgatattca tatcgcccat aatcagcacc ggattttcac
    23821 gcttgagttc ggtttccagg tagttttgca gattctgata aaactgcgcc ttcgcaggga
    23881 attttatcgg atggtcgcgg ctttcaccct gcgggaagta accgttgatc acggtgacat
    23941 tgcccagcgg tgaggggatt tccgccataa taatccgccg ttgcgcctct tcgtcgtcac
    24001 cgggaaagcc gcgacgcacg gcaatcggcg tctctttggt cagcagcgcc acgccataat
    24061 ggcctttctg cccgtgataa aacacgttgt agccgagctt cgccacctct tcgagcggaa
    24121 acatatcgtc atgaactttt gtctcctgca ggccaatcac atccggttgg tgcttttcga
    24181 cgatggcttc aagctggtga ggtctggcgc gcaggccgtt gatattaaaa gagacaaatt
    24241 tcatagtcgc tgccatttat tcagataaag agtgcgtgga tggtagcaga atttcgcttt
    24301 gcttaccgcc tgttgttaag tgatttgatc gatggttgtt aatgatgtca acgatggcgc
    24361 aaaaaatgcc cgcttttggt gcgcgttgcg tcagaatggc gcagtaattt ccagtaaatt
    24421 tcgtcaatga tcacaattcc agaattatat tttacttctg cataacattg cgttttttat
    24481 tctttttatt cacccacatc gcaaacgtat tcactttata tgcactttaa atgcatatgg
    24541 tttggttata actgcttgat ttccagttag cctccgccgt ttatgcactt ttatcactgg
    24601 ctggcacgaa ccctgcaatc tacatttaca gcgcaaacat tacttattat taacatataa
    24661 ataacgaatt atttactgta gaggtcgcta tgtctcagcc aattacgcgt gaaaactttg
    24721 atgaatggat gatacctgtt tacgctccgg caccgtttat accggtacgt ggcgaaggtt
    24781 cgcgcttgtg ggatcagcag gggaaagagt atatcgactt cgcgggtggc attgcggtga
    24841 acgcgctggg ccatgcgcat ccggaactgc gtgaggcgct gaacgaacag gcgagtaagt
    24901 tctggcatac cggcaacggt tacaccaacg agccggtact gcgactggcg aaaaaattga
    24961 tcgacgccac gtttgccgat cgcgtcttct tttgtaactc cggtgcggaa gccaacgaag
    25021 cggcgctaaa actggcgcgt aaattcgctc acgaccgcta cggcagccat aagagcggca
    25081 tcgtggcgtt caaaaatgcg tttcatggtc gcacgctgtt tactgtcagt gcgggtgggc
    25141 agccagccta ttcacaggat tttgcgccac tgccgccgga tattcatcat gctgcatata
    25201 acgatattaa ctctgccagt gcgctgattg acgacgccac ctgtgcggtg attgtcgaac
    25261 ccatccaggg ggaaggcggt gtggtgccag ccagcaacgc atttttacaa ggtctgcgtg
    25321 aattgtgtga ccgccacaat gcgttgctga tctttgatga agtacaaacc ggtgtcgggc
    25381 gcaccgggga actgtatgcc tatatgcact acggcgtgac gcctgatctg ttaactaccg
    25441 ccaaagcgct gggcggcggt ttcccggttg gtgcgttgtt ggcaaccgaa gagtgcgcca
    25501 gcgtgatgac cgttggcact cacggcacca cctatggcgg caatccgctg gcctcggcgg
    25561 tggcaggcaa agtgctggat ctcatcaaca cgccagagat gcttaacggg gttaaacagc
    25621 gtcacgactg gtttgttgag cgtctaaatt ccattaatca ccactacggt ttgttcagtg
    25681 aagttcgcgg cttaggtttg ttgattggtt gcgtgttgaa tgccgattac gccgggcaag
    25741 cgaaacagat ctctcaggaa gcggcgaaag caggcgtgat ggtactgatt gcgggtggca
    25801 acgtggtgcg ttttgcgcct gcgctcaatg tcagcgaaga agaggtgacg accggactgg
    25861 atcgctttgc agctgcttgc gaacactttg ttagccgagg ttcatcatga tggtcatccg
    25921 tcccgttgag cgctcagatg tctcggcgct gatgcagctt gccagcaaaa cgggcggcgg
    25981 cctgacgtcg cttcccgcca atgaagccac gctttcggcg cgtatcgaaa gggcaatcaa
    26041 aacctggcaa ggcgaactgc ccaaaagtga gcagggctat gtgttcgtgc tggaagatag
    26101 tgagacaggc actgtggcgg ggatttgcgc cattgaggtg gcggttgg
//
LOCUS       DAMZFT010000065        26119 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.065, whole genome shotgun sequence.
ACCESSION   DAMZFT010000065 DAMZFT010000000
VERSION     DAMZFT010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 26119)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26119)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26119
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.065"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            201..395
                     /gene="bfd"
                     /locus_tag="PN977_002756"
     CDS             201..395
                     /gene="bfd"
                     /locus_tag="PN977_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin-associated ferredoxin"
                     /protein_id="HDI6042774.1"
                     /translation="MYVCLCNGVSDKKIRQAVRQFHPQSFQQLRKFIPVGNQCGKCIR
                     AAREVMQDELMQMPEFKEIA"
     gene            468..944
                     /gene="bfr"
                     /locus_tag="PN977_002757"
     CDS             468..944
                     /gene="bfr"
                     /locus_tag="PN977_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HDI6042775.1"
                     /translation="MKGDVKIINYLNKLLGNELVAINQYFLHARMFKNWGLMRLNDVE
                     YHESIDEMKHADKYIERILFLEGIPNLQDLGKLGIGEDVEEMLQSDLRLELEGAKDLR
                     EAIAYADSVHDYVSRDMMIEILADEEGHIDWLETELDLIGKIGLQNYLQSQIKVKD"
     gene            complement(941..1408)
                     /locus_tag="PN977_002758"
     CDS             complement(941..1408)
                     /locus_tag="PN977_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HDI6042776.1"
                     /translation="MDATLPFLILYACLSALLFFWDAKHGLLPDRFTCPLLWSGLLFY
                     QVCNPDGLADALWGAIIGYGTFAVIYWGYRILRHKEGLGYGDVKFLAALGAWHTWTFL
                     PRLVFLAASFACGAVVIGLLMRGKESLKNPLPFGPFLAAAGFVVGWDSLLAGR"
     gene            1787..2098
                     /gene="rpsJ"
                     /locus_tag="PN977_002759"
     CDS             1787..2098
                     /gene="rpsJ"
                     /locus_tag="PN977_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014268624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HDI6042777.1"
                     /translation="MQNQRIRIRLKAFDHRLIDQATAEIVETAKRTGAQVRGPIPLPT
                     RKERFTVLISPHVNKDARDQYEIRTHLRLVDIVEPTEKTVDALMRLDLAAGVDVQISL
                     G"
     gene            2131..2760
                     /gene="rplC"
                     /locus_tag="PN977_002760"
     CDS             2131..2760
                     /gene="rplC"
                     /locus_tag="PN977_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000579825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HDI6042778.1"
                     /translation="MIGLVGKKVGMTRIFTEDGVSIPVTVIEVEANRVTQVKDLANDG
                     YRAIQVTTGAKKANRVTKPEAGHFAKAGVEAGRGLWEFRLAEGEEFTVGQSISVELFA
                     DVKKVDVTGTSKGKGFAGTVKRWNFRTQDATHGNSLSHRVPGSIGQNQTPGKVFKGKK
                     MAGQMGNERVTVQSLDVVRVDAERNLLLVKGAVPGATGSDLIVKPAVKA"
     gene            2771..3376
                     /gene="rplD"
                     /locus_tag="PN977_002761"
     CDS             2771..3376
                     /gene="rplD"
                     /locus_tag="PN977_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010304939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HDI6042779.1"
                     /translation="MELVLKDAQSALTVSETTFGRDFNEALVHQVVVAYAAGARQGTR
                     AQKTRAEVTGSGKKPWRQKGTGRARSGSIKSPIWRSGGVTFAARPQDHSQKVNKKMYR
                     GALKSILSELVRQDRLIVVEKFSVEAPKTKLLAQKLKDMALEDVLIITGELDENLFLA
                     ARNLHKVDVRDATGIDPVSLIAFDKVVMTADAVKQVEEMLA"
     gene            3373..3675
                     /gene="rplW"
                     /locus_tag="PN977_002762"
     CDS             3373..3675
                     /gene="rplW"
                     /locus_tag="PN977_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007891652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HDI6042780.1"
                     /translation="MIREERLLKVLRAPHVSEKASTAMEKSNTIVLKVAKDATKAEIK
                     AAVQKLFEVEVEVVNTLVVKGKVKRHGQRIGRRSDWKKAYVTLKEGQNLDFVGGAE"
     gene            3693..4514
                     /gene="rplB"
                     /locus_tag="PN977_002763"
     CDS             3693..4514
                     /gene="rplB"
                     /locus_tag="PN977_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HDI6042781.1"
                     /translation="MAVVKCKPTSPGRRHVVKVVNPELHKGKPFAPLLEKNSKSGGRN
                     NNGRITTRHIGGGHKQAYRIVDFKRNKDGIPAVVERLEYDPNRSANIALVLYKDGERR
                     YILAPKGLKAGDQIQSGVDAAIKPGNTLPMRNIPVGSTVHNVEMKPGKGGQLARSAGT
                     YVQIVARDGAYVTLRLRSGEMRKVEADCRATLGEVGNAEHMLRVLGKAGAARWRGVRP
                     TVRGTAMNPVDHPHGGGEGRNFGKHPVTPWGVQTKGKKTRSNKRTDKFIVRRRSK"
     gene            4531..4809
                     /gene="rpsS"
                     /locus_tag="PN977_002764"
     CDS             4531..4809
                     /gene="rpsS"
                     /locus_tag="PN977_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HDI6042782.1"
                     /translation="MPRSLKKGPFIDLHLLKKVEKAVESGDKKPLRTWSRRSTIFPNM
                     IGLTIAVHNGRQHVPVFVTDEMVGHKLGEFAPTRTYRGHAADKKAKKK"
     gene            4824..5156
                     /gene="rplV"
                     /locus_tag="PN977_002765"
     CDS             4824..5156
                     /gene="rplV"
                     /locus_tag="PN977_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HDI6042783.1"
                     /translation="METIAKHRHARSSAQKVRLVADLIRGKKVSQALDILTYTNKKAA
                     VLVKKVLESAIANAEHNDGADIDDLKVTKIFVDEGPSMKRIMPRAKGRADRILKRTSH
                     ITVVVSDR"
     gene            5174..5875
                     /gene="rpsC"
                     /locus_tag="PN977_002766"
     CDS             5174..5875
                     /gene="rpsC"
                     /locus_tag="PN977_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HDI6042784.1"
                     /translation="MGQKVHPNGIRLGIVKPWNSTWFANTKEFADNLDSDFKVRQYLT
                     KELAKASVSRIVIERPAKSIRVTIHTARPGIVIGKKGEDVEKLRKVVADIAGVPAQIN
                     IAEVRKPELDAKLVADSITSQLERRVMFRRAMKRAVQNAMRLGAKGIKVEVSGRLGGA
                     EIARTEWYREGRVPLHTLRADIDYNTSEAHTTYGVIGVKVWIFKGEILGGMAAVEQPE
                     KPAAQPKKQQRKGRK"
     gene            5888..6298
                     /gene="rplP"
                     /locus_tag="PN977_002767"
     CDS             5888..6298
                     /gene="rplP"
                     /locus_tag="PN977_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017805229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HDI6042785.1"
                     /translation="MLQPKRTKFRKMHKGRNRGLAQGTDVSFGSFGLKAVGRGRLTAR
                     QIEAARRAMTRAVKRQGKIWIRVFPDKPITEKPLAVRMGKGKGNVEYWVALIQPGKVL
                     YEMDGVPEELAREAFKLAAAKLPIKTTFVTKTVM"
     gene            6298..6489
                     /gene="rpmC"
                     /locus_tag="PN977_002768"
     CDS             6298..6489
                     /gene="rpmC"
                     /locus_tag="PN977_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000644743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HDI6042786.1"
                     /translation="MKAKELREKSVEELNTELLNLLREQFNLRMQAASGQLQQSHLLK
                     QVRRDVARVKTLLNEKAGA"
     gene            6489..6743
                     /gene="rpsQ"
                     /locus_tag="PN977_002769"
     CDS             6489..6743
                     /gene="rpsQ"
                     /locus_tag="PN977_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006817275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HDI6042787.1"
                     /translation="MTDKIRTLQGRVVSDKMEKSIVVAIERFVKHPIYGKFIKRTTKL
                     HVHDENNECGIGDVVEIRECRPLSKTKSWTLVRVVEKAVL"
     gene            6908..7279
                     /gene="rplN"
                     /locus_tag="PN977_002770"
     CDS             6908..7279
                     /gene="rplN"
                     /locus_tag="PN977_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015817037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HDI6042788.1"
                     /translation="MIQEQTMLNVADNSGARRVMCIKVLGGSHRRYAGVGDIIKITIK
                     EAIPRGKVKKGDVLKAVVVRTKKGVRRPDGSVIRFDGNACVLLNNNSEQPIGTRIFGP
                     VTRELRSEKFMKIISLAPEVL"
     gene            7290..7604
                     /gene="rplX"
                     /locus_tag="PN977_002771"
     CDS             7290..7604
                     /gene="rplX"
                     /locus_tag="PN977_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000729185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HDI6042789.1"
                     /translation="MAAKIRRDDEVIVLTGKDKGKRGKVKNVLSSGKVIVEGINLVKK
                     HQKPVPALNQPGGIVEKEAAIQVSNVAIFNAATGKADRVGFRFEDGKKVRFFKSNSET
                     IK"
     gene            7619..8158
                     /gene="rplE"
                     /locus_tag="PN977_002772"
     CDS             7619..8158
                     /gene="rplE"
                     /locus_tag="PN977_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HDI6042790.1"
                     /translation="MAKLHDYYKDEVVKKLMTEFNYNSVMQVPRVEKITLNMGVGEAI
                     ADKKLLDNAAADLAAISGQKPLITKARKSVAGFKIRQGYPIGCKVTLRGERMWEFFER
                     LITIAVPRIRDFRGLSAKSFDGRGNYSMGVREQIIFPEIDYDKVDRVRGLDITITTTA
                     KSDEEGRALLAAFDFPFRK"
     gene            8173..8478
                     /gene="rpsN"
                     /locus_tag="PN977_002773"
     CDS             8173..8478
                     /gene="rpsN"
                     /locus_tag="PN977_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HDI6042791.1"
                     /translation="MAKQSMKAREVKRVALADKYFAKRAELKAIISDVNASDEDRWNA
                     VLKLQTLPRDSSPSRQRNRCRQTGRPHGFLRKFGLSRIKVREAAMRGEIPGLKKASW"
     gene            8512..8904
                     /gene="rpsH"
                     /locus_tag="PN977_002774"
     CDS             8512..8904
                     /gene="rpsH"
                     /locus_tag="PN977_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HDI6042792.1"
                     /translation="MSMQDPIADMLTRIRNGQAANKAAVTMPSSKLKVAIANVLKEEG
                     FIEDFKVEGDTKPELELTLKYFQGKAVVESIQRVSRPGLRIYKRKDELPKVMAGLGIA
                     VVSTSKGVMTDRAARQAGLGGEIICYVA"
     gene            8917..9450
                     /gene="rplF"
                     /locus_tag="PN977_002775"
     CDS             8917..9450
                     /gene="rplF"
                     /locus_tag="PN977_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003031129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HDI6042793.1"
                     /translation="MSRVAKAPVVVPAGVDVKINGQVITIKGKNGELTRTLNDAVEVK
                     HADNTLTFGPRDGYADGWAQAGTARALLNSMVIGVTEGFTKKLQLVGVGYRAAVKGNV
                     INLSLGFSHPVDHQLPAGITAECPTQTEIVLKGADKQVIGQVAADLRAYRRPEPYKGK
                     GVRYADEVVRTKEAKKK"
     gene            9460..9813
                     /gene="rplR"
                     /locus_tag="PN977_002776"
     CDS             9460..9813
                     /gene="rplR"
                     /locus_tag="PN977_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HDI6042794.1"
                     /translation="MDKKSARIRRATRARRKLQELGATRLVVHRTPRHIYAQVIAPNG
                     SEVLVAASTVEKAIAEQLKYTGNKDAAAAVGKAVAERALEKGIKDVSFDRSGFQYHGR
                     VQALADAAREAGLQF"
     gene            9828..10331
                     /gene="rpsE"
                     /locus_tag="PN977_002777"
     CDS             9828..10331
                     /gene="rpsE"
                     /locus_tag="PN977_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004106339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HDI6042795.1"
                     /translation="MAHIEKQAGELQEKLIAVNRVSKTVKGGRIFSFTALTVVGDGNG
                     RVGFGYGKAREVPAAIQKAMEKARRNMINVALNNGTLQHPVKGVHTGSRVFMQPASEG
                     TGIIAGGAMRAVLEVAGVHNVLAKAYGSTNPINVVRATIDGLENMNSPEMVAAKRGKS
                     VEEILGK"
     gene            10335..10514
                     /gene="rpmD"
                     /locus_tag="PN977_002778"
     CDS             10335..10514
                     /gene="rpmD"
                     /locus_tag="PN977_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012147215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HDI6042796.1"
                     /translation="MAKTIKITQTRSAIGRLPKHKATLLGLGLRRIGHTVEREDTPAI
                     RGMINAVSFMVKVEE"
     gene            10518..10952
                     /gene="rplO"
                     /locus_tag="PN977_002779"
     CDS             10518..10952
                     /gene="rplO"
                     /locus_tag="PN977_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011095501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HDI6042797.1"
                     /translation="MRLNTLSPAEGSKKAGKRLGRGIGSGLGKTGGRGHKGQKSRSGG
                     GVRRGFEGGQMPLYRRLPKFGFTSRKAAITAEVRLSDLAKVEGGVVDLNTLKAANIIG
                     IQIEFAKVILAGEVTTPVTVRGLRVTKGARAAIEAAGGKIEE"
     gene            10960..12291
                     /gene="secY"
                     /locus_tag="PN977_002780"
     CDS             10960..12291
                     /gene="secY"
                     /locus_tag="PN977_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004868351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HDI6042798.1"
                     /translation="MAKQPGLDFQSAKGGLGELKRRLLFVIGALIVFRIGSFIPIPGI
                     DAAVLAKLLEQQRGTIIEMFNMFSGGALSRASIFALGIMPYISASIIIQLLTVVHPTL
                     AEIKKEGESGRRKISQYTRYGTLVLAIFQSIGIATGLPNMPGMQGLVINPGFAFYFTA
                     VVSLVTGTMFLMWLGEQITERGIGNGISIIIFAGIVAGLPPAIAHTIEQARQGDLHFL
                     VLLLVAVLVFAVTFFVVFVERGQRRIVVNYAKRQQGRRVYAAQSTHLPLKVNMAGVIP
                     AIFASSIILFPATIASWFGGGTGWNWLTTISLYLQPGQPLYVLLYASAIIFFCFFYTA
                     LVFNPRETADNLKKSGAFVPGIRPGEQTAKYIDKVMTRLTLVGALYITFICLIPEFMR
                     DAMKVPFYFGGTSLLIVVVVIMDFMAQVQTLMMSSQYESALKKANLKGYGR"
     gene            12323..12439
                     /gene="rpmJ"
                     /locus_tag="PN977_002781"
     CDS             12323..12439
                     /gene="rpmJ"
                     /locus_tag="PN977_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020322760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HDI6042799.1"
                     /translation="MKVRASVKKLCRNCKIVKRDGVIRVICSAEPKHKQRQG"
     gene            12586..12942
                     /gene="rpsM"
                     /locus_tag="PN977_002782"
     CDS             12586..12942
                     /gene="rpsM"
                     /locus_tag="PN977_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019935869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HDI6042800.1"
                     /translation="MARIAGINIPDHKHAVIALTSIYGVGKTRSKAILAAAGIAEDVK
                     ISELSEGQIDTLRDEVAKFVVEGDLRREISMSIKRLMDLGCYRGLRHRRGLPVRGQRT
                     KTNARTRKGPRKPIKK"
     gene            12959..13348
                     /gene="rpsK"
                     /locus_tag="PN977_002783"
     CDS             12959..13348
                     /gene="rpsK"
                     /locus_tag="PN977_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HDI6042801.1"
                     /translation="MAKAPIRARKRVRKQVSDGVAHIHASFNNTIVTITDRQGNALGW
                     ATAGGSGFRGSRKSTPFAAQVAAERCADAVKEYGIKNLEVMVKGPGPGRESTIRALNA
                     AGFRITNITDVTPIPHNGCRPPKKRRV"
     gene            13382..14002
                     /gene="rpsD"
                     /locus_tag="PN977_002784"
     CDS             13382..14002
                     /gene="rpsD"
                     /locus_tag="PN977_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004410410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HDI6042802.1"
                     /translation="MARYLGPKLKLSRREGTDLFLKSGVRAIDTKCKIEQAPGQHGAR
                     KPRLSDYGVQLREKQKVRRIYGVLERQFRNYYKEAARLKGNTGENLLALLEGRLDNVV
                     YRMGFGATRAEARQLVSHKAIMVNGRVVNIASYQVSPNDVVSIREKAKKQSRVKAALE
                     LAEQREKPTWLEVDAGKMEGTFKRKPERSDLSADINEHLIVELYSK"
     gene            14028..15017
                     /gene="rpoA"
                     /locus_tag="PN977_002785"
     CDS             14028..15017
                     /gene="rpoA"
                     /locus_tag="PN977_002785"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HDI6042803.1"
                     /translation="MQGSVTEFLKPRLVDIEQVSSTHAKVTLEPLERGFGHTLGNALR
                     RILLSSMPGCAVTEVEIDGVLHEYSTKEGVQEDILEILLNLKGLAVRVQGKDEVILTL
                     NKSGIGPVTAADITHDGDVEIVKPQHVICHLTDENASISMRIKVQRGRGYVPASTRIH
                     SEEDERPIGRLLVDACYSPVERIAYNVEAARVEQRTDLDKLVIEMETNGTIDPEEAIR
                     RAATILAEQLEAFVDLRDVRQPEVKEEKPEFDPILLRPVDDLELTVRSANCLKAEAIH
                     YIGDLVQRTEVELLKTPNLGKKSLTEIKDVLASRGLSLGMRLENWPPASIADE"
     gene            15058..15441
                     /gene="rplQ"
                     /locus_tag="PN977_002786"
     CDS             15058..15441
                     /gene="rplQ"
                     /locus_tag="PN977_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HDI6042804.1"
                     /translation="MRHRKSGRQLNRNSSHRQAMFRNMAGSLVRHEIIKTTLPKAKEL
                     RRVVEPLITLAKTDSVANRRLAFARTRDNEIVAKLFNELGPRFASRAGGYTRILKCGF
                     RAGDNAPMAYIELVDRSEKAEAAAE"
     gene            15548..15916
                     /locus_tag="PN977_002787"
     CDS             15548..15916
                     /locus_tag="PN977_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1992 domain-containing protein"
                     /protein_id="HDI6042805.1"
                     /translation="MWLLDQWAERHITEAQSKGEFDNLPGSGEPLILDDDSHVPPELR
                     AGYRLLKNAGCLPPELEQRREAIQLLDILKGIRHDDPQYKEVSRRLSLLELKLRQAGL
                     STDFLRGDYADKLLDKIYDN"
     gene            15927..16352
                     /gene="zntR"
                     /locus_tag="PN977_002788"
     CDS             15927..16352
                     /gene="zntR"
                     /locus_tag="PN977_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(2+)-responsive transcriptional regulator"
                     /protein_id="HDI6042806.1"
                     /translation="MYRIGELAKMAEVTPDTIRYYEKQQMMEHEVRTEGGFRLYTESD
                     LQRLKFIRHARQLGFSLESIRELLSIRIDPEHHTCQESKGIVQERLQEVEARIAELQS
                     MQRSLQRLNDACCGTAHSSVYCSILEALEQGASGVKSGC"
     gene            16408..16626
                     /gene="arfA"
                     /locus_tag="PN977_002789"
     CDS             16408..16626
                     /gene="arfA"
                     /locus_tag="PN977_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome-rescue factor ArfA"
                     /protein_id="HDI6042807.1"
                     /translation="MSRYQHTKGQIKDNAIEALLHDPLFRQRVERNKKGKGSYMRKGK
                     HGNRGNWEASGKKVNHFFTTGLLLSGAC"
     gene            complement(16623..17033)
                     /gene="mscL"
                     /locus_tag="PN977_002790"
     CDS             complement(16623..17033)
                     /gene="mscL"
                     /locus_tag="PN977_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large-conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HDI6042808.1"
                     /translation="MSIIKEFREFAMRGNVVDLAVGVIIGAAFGKIVSSLVADIIMPP
                     LGLLIGGIDFKQFAVTLRDAQGDIPAVVMHYGVFIQNVFDFLIVAFAIFMAIKLINKL
                     NRKKEEPAAAPAPTKEEVLLTEIRDLLKEQNNRS"
     gene            complement(17163..18539)
                     /gene="trkA"
                     /locus_tag="PN977_002791"
     CDS             complement(17163..18539)
                     /gene="trkA"
                     /locus_tag="PN977_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HDI6042809.1"
                     /translation="MKIIILGAGQVGGTLAENLVGENNDITVVDTNGERLRTLQDKFD
                     LRVVQGHGSHPRVLREAGADDADMLVAVTSSDETNMVACQVAYSLFNTPNRIARIRSP
                     DYVRDADKLFHSDAVPIDHLIAPEQLVIDNIYRLIEYPGALQVVNFAEGKVSLAVVKA
                     YYGGPLIGNALSTMREHMPHIDTRVAAIFRHDRPIRPQGSTIVEAGDEVFFIAASQHI
                     RAVMSELQRLEKPYKRIMLVGGGNIGAGLARRLEKDYSVKLIERNQQRAAELAEKLQN
                     TIVFFGDASDQELLAEEHIDQVDLFIAVTNDDEANIMSAMLAKRMGAKKVMVLIQRRA
                     YVDLVQGSVIDIAISPQQATISALLSHVRKADIVGVSSLRRGVAEAIEAVAHGDESTS
                     RVVGRVIDEIKLPPGTIIGAVVRGNDVMIANDNLRIEQGDHVIMFLTDKKFITDVERL
                     FQPSPFFL"
     gene            complement(18561..19850)
                     /gene="rsmB"
                     /locus_tag="PN977_002792"
     CDS             complement(18561..19850)
                     /gene="rsmB"
                     /locus_tag="PN977_002792"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HDI6042810.1"
                     /translation="MKKQRNLRSMAAQAVEQVVEQGQSLSNILPPLQQKVSDKDKALL
                     QELCFGVLRTLSQLDWLINKLMARPMTGKQRTVHYLIMVGLYQLLYTRIPPHAALAET
                     VEGAIAIKRPQLKGLINGVLRQFQRQQEELLAEFNASDARYLHPSWLLKRLQKAYPEQ
                     WQSIVEANNQRPPMWLRVNRTHHSRDRWLALLDEAGMKGFPHADYPDAVRLETPAPVH
                     ALPGFEDGWVTVQDASAQGCMTWLAPQNGEHILDLCAAPGGKTTHILEVAPEAQVVAV
                     DIDEQRLSRVYDNLKRLGMKATVKQGDGRYPSQWCGEQQFDRILLDAPCSATGVIRRH
                     PDIKWLRRDRDIPELAQLQSEILDAIWPHLKSGGTLVYATCSVLPEENSLQIKAFLQR
                     TADAELCETGTPEQPGKQNLPGAEEGDGFFYAKLIKK"
     gene            complement(19896..20843)
                     /gene="fmt"
                     /locus_tag="PN977_002793"
     CDS             complement(19896..20843)
                     /gene="fmt"
                     /locus_tag="PN977_002793"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HDI6042811.1"
                     /translation="MSESLRIIFAGTPDFAARHLDALLSSGHNVVGVFTQPDRPAGRG
                     KKLMPSPVKVLAEEKGLPVFQPVSLRPQENQQLVADLQADVMVVVAYGLILPKAVLEM
                     PRLGCINVHGSLLPRWRGAAPIQRSLWAGDAETGVTIMQMDVGLDTGDMLYKLSSPIT
                     AEDTSGTLYDKLAELGPQGLITTLKQLADGTAKPEVQDETLVTYAEKLSKEEARIDWS
                     LSAAQLERCIRAFNPWPMSWLEIEGQPVKVWKASVIDTVTKSAPGTILEASKQGIQVA
                     TGDGILNLLSLQPAGKKAMSAQDLLNSRREWFVPGNRLA"
     gene            complement(20858..21367)
                     /gene="def"
                     /locus_tag="PN977_002794"
     CDS             complement(20858..21367)
                     /gene="def"
                     /locus_tag="PN977_002794"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HDI6042812.1"
                     /translation="MSVLQVLHIPDERLRKVAKPVEEVNAEIQRIVDDMFETMYAEEG
                     IGLAATQVDIHQRIIVIDVSENRDERLVLINPELLEKSGETGIEEGCLSIPEQRALVP
                     RAEKVKIRALDRDGKPFELEAEGLLAICIQHEMDHLVGKLFMDYLSPLKQQRIRQKVE
                     KLDRLKARA"
     gene            21497..22621
                     /gene="dprA"
                     /locus_tag="PN977_002795"
     CDS             21497..22621
                     /gene="dprA"
                     /locus_tag="PN977_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HDI6042813.1"
                     /translation="MVDTEIWLRLMSISSLYGDDMVRIAHWLAKQSHIDAVVLQQTGL
                     TLRQAQRFLSFPRKSIESSLSWLEQPNHHLIPADSEFYPPQLLATTDYPGALFVEGEL
                     HALHSFQLAVVGSRAHSWYGERWGRLFCETLATRGVTITSGLARGIDGVAHKAALQVN
                     GVSIAVLGNGLNTIHPRRHARLATSLLEHGGALVSEFPLDVPPLAYNFPRRNRIISGL
                     SKGVLVVEAALRSGSLVTARCALEQGREVFALPGPLGNPGSEGPHWLIKQGAILVTEP
                     EEILENLQFGLHWLPDAPENSFYSPDQEDVALPFPELLANVGDEVTPVDVVAERAGQP
                     VPEVVTQLLELELAGWIAAVPGGYVRLRRACHVRRTNVFV"
     gene            22593..23066
                     /locus_tag="PN977_002796"
     CDS             22593..23066
                     /locus_tag="PN977_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF494 domain-containing protein"
                     /protein_id="HDI6042814.1"
                     /translation="MFDVLMYLFETYIHTEAELRVDQDKLEQDLTDAGFDREDIYNAL
                     LWLEKLADYQEGLAEPMQLASDPLSMRIYTPEECERLDASCRGFLLFLEQIQVLNLET
                     REMVIERVLALDTAEFDLEDLKWVILMVLFNIPGCENAYQQMEELLFEVNEGMLH"
     gene            23095..23637
                     /locus_tag="PN977_002797"
     CDS             23095..23637
                     /locus_tag="PN977_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase DNA-binding C4 zinc finger
                     domain-containing protein"
                     /protein_id="HDI6042815.1"
                     /translation="MAKSALFTVRNNESCPKCGAELVIRSGKHGPFLGCSQYPACDYV
                     RPLKSSADGHIVKVLEGQVCPACGAHLVLRQGRFGMFIGCSNYPECEHTELIDKPDET
                     AITCPQCRTGHLVQRRTRYGKTFHSCDRYPECQFAINFKPIAGECPECHYPLLIEKKT
                     AQGVKHFCASKQCGKPVSAE"
     gene            23642..24214
                     /gene="tsaC"
                     /locus_tag="PN977_002798"
     CDS             23642..24214
                     /gene="tsaC"
                     /locus_tag="PN977_002798"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase type 1
                     TsaC"
                     /protein_id="HDI6042816.1"
                     /translation="MNNNLQGDAIAAAIDVLNEERVIAYPTEAVFGVGCDPDSETAVM
                     RLLELKQRPVDKGLILIAANYEQLKPYIDDTMLTDAQRETIFSRWPGPVTFVFPAPAT
                     TPRWLTGRFDSLAVRVTDHPLVVALCQAYGKPLVSTSANLSGLPPCRTVDEVRAQFGA
                     AFPVVPGETGGRLNPSEIRDALTGELFRQG"
     gene            24219..25037
                     /gene="aroE"
                     /locus_tag="PN977_002799"
     CDS             24219..25037
                     /gene="aroE"
                     /locus_tag="PN977_002799"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HDI6042817.1"
                     /translation="METYAVFGNPIAHSKSPFIHQQFAQQLNIEHPYGRVLAPINDFI
                     NTLNAFFSAGGKGANVTVPFKEEAFARADELTERATLAGAVNTLKRLEDGRLLGDNTD
                     GIGLLSDLERLSFIRPGLRILLIGAGGASRGVLLPLLSLDCAVTITNRTVSRAEELAK
                     LFAHTGSIHALGMDELEGHEFDLIINATSSGISGDIPAIPASLIHSGMCCYDMFYQKG
                     KTPFLAWCELRGSKRNADGLGMLVAQAAHAFLLWHGVLPDIEPVIKQLQEELSA"
     gene            25034..25291
                     /locus_tag="PN977_002800"
     CDS             25034..25291
                     /locus_tag="PN977_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1488 domain-containing protein"
                     /protein_id="HDI6042818.1"
                     /translation="MNQAIQFPDREEWNESKKCVCFPALVNGMQLTCAITGESLANRF
                     AGDTPEQWLASFRQHRWDLEEEAENLIQEQSEDDQGWVWLP"
     gene            complement(25267..25821)
                     /locus_tag="PN977_002801"
     CDS             complement(25267..25821)
                     /locus_tag="PN977_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HDI6042819.1"
                     /translation="MSDVLRPYRDLFPQIGQRVMIDDSSVVIGDVRLADDVGIWPLVV
                     IRGDVHYVQIGARTNIQDGSMLHVTHKSSYNPAGNPLTIGEDVTVGHKVMLHGCTIGN
                     RVLVGMGSILLDGAIVEDDVMIGAGSLVPQNKRLESGYLYLGSPVKQIRPLSDEEKAG
                     LRYSANNYVKWKDEYMDQGNHTQP"
BASE COUNT         6702 a         6195 c         6763 g         6459 t
ORIGIN      
        1 gctaaagttc tgggctaatt acacgttaat tagtttcgaa ttaaaaaggg cgcttcggcg
       61 tcctttttgc tgcccgtaat ttgagctcga aatatttagt cgtaatttca ctcgctgaac
      121 tatggcgcaa attaaactgt gctattgtaa tcataaccat tctcatttac actttgcgca
      181 gaatttgaac gggagcaatc atgtacgttt gtttgtgtaa tggtgtaagt gacaaaaaaa
      241 ttcgccaggc agtccgtcag tttcatccgc aatctttcca acaattgcgt aagtttattc
      301 ctgtgggaaa tcaatgcggt aagtgcattc gcgccgcgcg cgaagtgatg caggacgagt
      361 tgatgcagat gccagaattt aaagagattg cctgaagccc acgcttattt tttgacatcc
      421 cgctagcccc atctacgctc taatagtgga agcggaggga ctataaaatg aaaggtgatg
      481 ttaaaatcat aaattatctc aataaactat tgggaaatga gcttgtcgca atcaatcagt
      541 attttctcca tgcccggatg tttaaaaact ggggcctgat gcgtctcaat gacgtcgaat
      601 accatgaatc tattgatgag atgaaacacg ctgataaata tatcgagcgt attttgtttt
      661 tagaaggaat ccctaactta caggatttag gcaaactggg gattggtgaa gatgtcgagg
      721 agatgctgca atccgacctg cgattagaac tggaaggcgc gaaggatctc cgtgaggcta
      781 tcgcgtatgc cgacagcgtt catgactatg ttagccgtga tatgatgatc gagatccttg
      841 ccgatgaaga aggtcatatt gactggctcg aaactgagct ggatctcatc ggtaaaatcg
      901 gtttacaaaa ttacctgcaa tcacaaataa aagttaaaga ttaacggcct gccagcaaac
      961 tatcccagcc tacaacgaaa cccgcagccg ccagaaatgg cccaaacggc agcgggtttt
     1021 ttaatgactc ttttcctctc atgagcagac caataactac ggctccgcaa gcgaatgagg
     1081 cagcaaggaa aaccagtcgt ggcaagaagg tccatgtatg ccaggcacca agggcggcga
     1141 gaaacttcac atcaccatag cctaatcctt ctttatgacg cagtatgcga tacccccagt
     1201 aaatgacagc gaatgtgccg taaccaataa tcgcccccca taacgcatcg gctaaaccgt
     1261 caggattaca aacctgatag aacaaaagac ctgaccagag taacgggcag gtaaatctgt
     1321 caggtaacaa gccatgcttt gcatcccaga aaaaaagtag ggctgataaa caagcgtaca
     1381 aaatcagaaa gggaagggta gcgtccataa aacatcctct gtaattggag tatcaccatc
     1441 gaataactcc ggatgctcag caacagagaa atcgctgatt tgttaacctg gtcccggtga
     1501 aaaacgctat cttcacaact ttacaaattt cgtggaatac agctcgcaat ctgcgcgttg
     1561 cgattaattt ctacccgatg cttgcgtcag gctcagattt gcgtataatg cgcgggcttg
     1621 tcgtagttga cagcaggttc aatctgaacc tcagttagcc gaatttggct acctaacaat
     1681 gctcccaatc ggggagctac gtaagaacgg ttacactctc ccatcaatcg taatgggtct
     1741 gaggagtaat cattttcgtt tataaaataa ttggagctct ggtctcatgc agaaccaaag
     1801 aatccgtatc cgcctgaaag cgtttgatca tcgtctgatc gatcaagcaa ccgcggaaat
     1861 cgtcgagact gccaagcgca ctggtgcgca ggtccgtggt ccgatcccgc tgccgacacg
     1921 caaagagcgc ttcactgttc tgatctcccc gcacgtcaac aaagacgcgc gcgatcagta
     1981 cgaaatccgt actcacttgc gtctggttga catcgttgag ccaaccgaga aaaccgttga
     2041 tgctctgatg cgtctggatc tggctgccgg tgtagacgtg cagatcagcc tgggttaatc
     2101 aggtcattga gcgattgaga ggttgaaaca atgattggtt tagtcggtaa aaaagtgggt
     2161 atgacccgta tcttcacaga agacggcgtt tctatcccag taaccgtaat cgaagttgaa
     2221 gcaaaccgcg ttactcaggt taaagacctg gctaacgatg gctaccgtgc tattcaggta
     2281 accaccggtg ctaaaaaagc taaccgtgtg accaagcctg aagctggcca cttcgctaaa
     2341 gctggcgtag aagctggccg tggtctgtgg gaattccgcc tggctgaagg cgaagagttc
     2401 actgtaggtc agagcattag cgttgaactg tttgctgacg ttaaaaaagt tgacgtaact
     2461 ggcacctcta aaggtaaagg tttcgcaggt accgttaagc gctggaactt ccgtacccag
     2521 gacgctactc acggtaactc cttgtctcac cgcgttccgg gttctatcgg tcagaaccag
     2581 actccgggca aagtgttcaa aggcaagaaa atggcaggtc agatgggtaa cgaacgtgta
     2641 accgttcaga gccttgacgt agtacgcgtt gacgctgagc gcaacctgct gctggttaaa
     2701 ggtgctgtcc cgggtgcaac cggtagcgac ctgatcgtta aaccagctgt gaaggcgtaa
     2761 ggagatagca atggaattag tattgaaaga cgcgcagagc gcgctgactg tttccgaaac
     2821 taccttcggt cgtgatttca acgaagcgct ggttcaccag gttgttgttg cttatgcagc
     2881 tggtgctcgt cagggtactc gtgctcagaa gactcgtgct gaagtaactg gttccggtaa
     2941 aaaaccgtgg cgccagaaag gcaccggccg tgcgcgttct ggttctatca agagcccgat
     3001 ctggcgttct ggtggcgtga cctttgctgc tcgtccgcag gaccacagtc aaaaagttaa
     3061 caagaagatg taccgcggcg cgctgaaaag catcctgtcc gaactggtac gtcaggatcg
     3121 tctgatcgtt gtcgagaagt tctctgtaga agcgccgaaa actaagctgc tggcacagaa
     3181 actgaaagac atggctctgg aagatgtgct gatcatcacc ggtgagctgg acgaaaacct
     3241 gttcctggct gcgcgcaacc tgcacaaggt tgacgtacgc gatgcaactg gtatcgaccc
     3301 ggttagcctg atcgccttcg acaaagtcgt aatgactgct gatgctgtta agcaagttga
     3361 ggagatgctg gcatgattcg tgaagaacgt ctgctgaagg tgctgcgtgc accgcacgtt
     3421 tctgaaaaag cgtctactgc gatggaaaaa tctaacacca tcgtactcaa agttgctaaa
     3481 gacgcgacca aagcagaaat caaagctgct gtgcagaaac tgtttgaagt cgaagtcgaa
     3541 gtcgttaaca ccctggtagt taaagggaaa gttaaacgtc acggacagcg tatcggtcgt
     3601 cgtagcgact ggaaaaaagc ttacgtcacc ctgaaagaag gccagaatct ggacttcgtt
     3661 ggcggcgctg agtaagtcgg aggagtaata caatggcagt tgttaaatgt aaaccgacat
     3721 ctccgggtcg tcgccacgtt gttaaagtgg ttaaccctga gctgcacaag ggcaaacctt
     3781 ttgctccgtt gctggaaaaa aacagcaaat ccggtggtcg taacaacaat ggccgtatca
     3841 ccactcgtca tatcggtggt ggccacaagc aggcttaccg tattgttgac ttcaaacgca
     3901 acaaagacgg tatcccggca gttgttgaac gtcttgagta cgatccgaac cgttccgcga
     3961 acatcgcgct ggttctgtac aaagacggtg aacgccgtta catcctggcc cctaaaggcc
     4021 tgaaagctgg cgaccagatt cagtctggcg ttgatgctgc aatcaaacca ggtaacaccc
     4081 tgccgatgcg caacatcccg gttggttcta ctgttcataa cgtagaaatg aaaccaggta
     4141 aaggcggtca gctggcacgt tccgctggta cttacgttca gatcgttgct cgtgatggtg
     4201 cttatgtcac cctgcgtctg cgttctggtg aaatgcgtaa agtagaagca gactgccgtg
     4261 caactctggg cgaagttggc aatgctgagc atatgctgcg cgttctgggt aaagcaggtg
     4321 ctgcacgctg gcgtggtgtt cgtccgaccg ttcgcggtac cgcgatgaac ccggtagacc
     4381 acccacatgg tggtggtgaa ggtcgtaact ttggtaagca cccggtaact ccgtggggcg
     4441 ttcagaccaa aggtaagaag acccgcagca acaagcgtac tgataaattc atcgtacgtc
     4501 gccgtagcaa ataattttag aggataagcc atgccacgtt ctctcaagaa aggtcctttt
     4561 attgacctgc acttgctgaa gaaggtagag aaagcggtgg aaagcggaga caagaagccc
     4621 ctgcgcactt ggtcccgtcg ttcaacgatc tttcctaaca tgatcggttt gaccatcgct
     4681 gtccataatg gtcgtcagca cgttccggta tttgtaaccg acgaaatggt cggtcacaaa
     4741 ctgggtgaat tcgcaccgac tcgtacttat cgcggccacg ctgctgataa aaaagcgaag
     4801 aagaaataag gtaggaggaa gagatggaaa ctatcgctaa acatcgccat gctcgttctt
     4861 ctgctcagaa ggttcgcctt gttgctgacc tgattcgcgg taagaaagtg tcgcaggctc
     4921 tggatatttt gacctacacc aacaagaaag cggctgtact ggtcaagaaa gttctggaat
     4981 ctgccattgc taacgctgaa cacaacgatg gcgctgacat tgacgatctg aaagttacga
     5041 aaattttcgt agacgaaggc ccgagcatga agcgcattat gccgcgtgca aaaggtcgtg
     5101 cagatcgcat cctgaagcgc accagccaca tcactgtggt tgtgtccgat cgctgagact
     5161 ctggagacta gcaatgggtc agaaagtaca tcctaatggt attcgcctgg gtattgtaaa
     5221 accatggaac tctacctggt ttgcgaacac caaagaattc gctgacaacc tggacagcga
     5281 ttttaaagta cgtcagtacc tgactaagga actggctaaa gcgtccgtat ctcgtatcgt
     5341 tatcgagcgt ccggctaaga gcatccgtgt aaccattcac actgctcgcc cgggtatcgt
     5401 tatcggtaaa aaaggtgaag acgtagaaaa actgcgtaag gtcgtagcgg acatcgctgg
     5461 cgttcctgca cagatcaaca tcgccgaagt tcgtaagcct gaactggacg caaaactggt
     5521 tgctgacagc atcacttctc agctggaacg tcgcgttatg ttccgtcgtg ctatgaagcg
     5581 tgctgtacag aacgcaatgc gtctgggcgc taaaggtatt aaagttgaag ttagcggccg
     5641 tctgggcggc gcggaaatcg cacgtaccga atggtaccgc gaaggtcgcg taccgctgca
     5701 cactctgcgt gctgacatcg actacaacac ctctgaagcg cacaccactt acggtgtaat
     5761 cggcgttaaa gtgtggatct tcaaaggcga gatcctgggt ggtatggctg ctgttgaaca
     5821 accggaaaaa ccggctgctc agcctaaaaa gcagcagcgt aaaggccgta aataaggagc
     5881 gtcgctgatg ttacaaccaa agcgtacaaa attccgtaaa atgcacaaag gccgtaaccg
     5941 cggtctggcg cagggtacgg atgttagctt cggcagcttc ggtctgaaag ctgttggccg
     6001 tggtcgtctg actgcacgtc agatcgaagc agcacgtcgt gctatgaccc gtgcagttaa
     6061 gcgtcaaggt aagatctgga tccgtgtgtt cccggacaaa ccgatcactg aaaagccgct
     6121 ggcagtgcgt atgggtaaag gtaaaggtaa cgtggagtat tgggttgcct tgattcagcc
     6181 gggtaaagtc ctgtatgaaa tggacggtgt tccggaagag ctggcccgtg aagcattcaa
     6241 gctggcagca gcgaaactgc cgattaaaac cacctttgta actaagacgg tgatgtaatg
     6301 aaagcaaaag agctgcgtga gaagagcgtt gaagagctga acaccgagct gctgaacctg
     6361 ctgcgtgagc agttcaacct gcgtatgcag gctgcaagtg gccagctgca acagtctcac
     6421 ctgttgaagc aagtgcgtcg cgatgtcgca cgcgttaaga ctttactgaa cgagaaggcg
     6481 ggtgcgtaat gaccgataaa atccgtactc tgcaaggtcg cgttgttagc gacaaaatgg
     6541 agaaatccat tgttgttgct atcgaacgtt ttgtgaaaca cccgatctac ggtaaattca
     6601 tcaagcgtac gaccaaactg cacgtacatg acgagaacaa cgaatgcggt atcggtgacg
     6661 tggttgaaat ccgcgaatgc cgtccgctgt ccaagactaa atcctggacg ctggttcgcg
     6721 ttgtagagaa agcggttctg taatacagta cactctctcg atacgaataa acggctcaga
     6781 aatgagccgt ttattttttc tacccatatc cttgaagcgg tgttataatg ccgcgccctc
     6841 gatatgggga tttttaacga cctgattttc gggtctcagt agtagttgac attagcggag
     6901 cactaaaatg atccaagaac agactatgct gaacgtcgcc gacaactccg gtgcacgtcg
     6961 cgtaatgtgt atcaaggttc tgggtggctc gcaccgtcgc tacgcaggcg taggcgacat
     7021 catcaagatc accatcaaag aagcaattcc gcgtggtaag gtcaaaaaag gtgatgtgct
     7081 gaaggcggta gtggtgcgca ccaagaaggg tgttcgtcgc ccggacggtt ctgtcattcg
     7141 cttcgatggt aatgcttgtg ttcttctgaa caacaacagc gagcagccta tcggtacgcg
     7201 tatttttggg ccggtaactc gtgagcttcg tagtgagaag ttcatgaaaa ttatctctct
     7261 ggcaccagaa gtactctaag gagcgaatca tggcagcgaa aatccgtcgt gatgacgaag
     7321 ttatcgtgtt aaccggtaaa gataaaggta aacgcggtaa agttaagaat gtcctgtctt
     7381 ccggcaaggt cattgttgaa ggtatcaacc tggttaagaa acatcagaag ccggttccgg
     7441 ccctgaacca accgggtggc atcgttgaaa aagaagccgc tattcaggtt tccaacgtag
     7501 caatcttcaa tgcggcaacc ggcaaggctg accgtgtagg ctttagattc gaagacggta
     7561 aaaaagtccg tttcttcaag tctaacagcg aaactatcaa gtaatttgga gtagtacgat
     7621 ggcgaaactg catgattact acaaagacga agtagttaaa aaactcatga ctgagtttaa
     7681 ctacaattct gtcatgcaag tccctcgggt cgagaagatc accctgaaca tgggtgttgg
     7741 tgaagcgatc gctgacaaaa aactgctgga taacgcagca gcagacctgg cagcaatctc
     7801 tggtcaaaaa ccgctgatca ccaaagcacg caaatctgtt gcaggcttca aaatccgtca
     7861 gggctatccg atcggctgta aagtaactct gcgtggcgaa cgcatgtggg agttctttga
     7921 gcgcctgatc actattgctg tacctcgtat ccgtgacttc cgtggcctgt ccgctaagtc
     7981 tttcgacggt cgtggtaact acagcatggg tgtccgtgag cagatcatct tcccagaaat
     8041 cgactacgat aaagtcgacc gcgttcgtgg tttggatatt accattacca ctactgcgaa
     8101 atctgacgaa gaaggccgcg ctctgctggc tgcctttgac ttcccgttcc gcaagtaagg
     8161 tagggttact aaatggctaa gcaatcaatg aaagcacgcg aagtaaaacg cgtagcttta
     8221 gctgataaat acttcgcgaa acgcgctgaa ctgaaagcga tcatctctga tgtgaacgct
     8281 tccgacgaag atcgttggaa cgctgttctc aagctgcaga ctctgccgcg tgattccagc
     8341 ccgtctcgtc agcgtaaccg ctgccgtcaa acaggtcgtc cgcatggttt cctgcggaag
     8401 ttcgggttga gccgtattaa ggtccgtgaa gccgctatgc gcggtgaaat cccgggtctg
     8461 aaaaaggcta gctggtaatt gtcaccaatt gaatcacggg agtaaagaca gatgagcatg
     8521 caagatccga tcgcggatat gctgacccgt atccgtaacg gtcaggccgc gaacaaagct
     8581 gcggtcacca tgccttcctc caagctgaaa gtggcaatcg ccaacgtgct gaaggaagaa
     8641 ggttttattg aagattttaa agttgaaggc gacaccaagc ctgaactgga acttactctg
     8701 aagtatttcc agggcaaagc tgttgtagaa agcattcagc gtgtcagccg cccaggtctg
     8761 cgcatctata aacgtaaaga tgagctgccg aaagttatgg cgggtctggg tatcgcagtt
     8821 gtttctacct ctaaaggtgt tatgactgat cgtgcagcgc gccaggctgg tcttggtggc
     8881 gaaattatct gctacgtagc ctaatcggag gaaaaaatgt ctcgtgttgc taaagcaccg
     8941 gtcgttgttc ctgccggcgt tgatgtaaaa atcaacggtc aggttattac gatcaaaggt
     9001 aaaaacggcg agctgactcg tactctcaac gatgctgttg aagttaaaca tgcagataat
     9061 accctgacct tcggtccgcg tgatggttac gcagacggtt gggcacaggc tggtaccgcg
     9121 cgtgccctgc tgaactcaat ggttatcggt gttaccgaag gcttcactaa gaagctgcag
     9181 ctggttggtg taggttaccg tgcagcggtt aaaggcaatg tgattaacct gtctctgggt
     9241 ttctctcatc ctgttgacca tcagctgcct gcgggtatca ctgctgaatg tccgactcag
     9301 actgaaatcg tgctgaaagg cgctgataag caggtgattg gccaggttgc agcggatctg
     9361 cgcgcctacc gtcgtcctga gccttacaaa ggcaagggtg ttcgttacgc cgacgaagtc
     9421 gtgcgtacca aagaggctaa gaagaagtaa ggtaacacta tggataagaa atctgctcgt
     9481 atccgtcgtg cgacccgcgc acgccgcaag ctccaggagc tgggcgcaac tcgcctggtg
     9541 gtacatcgta ccccgcgtca catttacgca caggtaattg caccgaacgg ttctgaagtt
     9601 ctggtagctg cttctactgt agaaaaagct atcgctgaac aactgaagta caccggtaac
     9661 aaagacgcgg ctgcagctgt gggtaaagct gtcgctgaac gcgctctgga aaaaggcatc
     9721 aaagatgtat cctttgaccg ttccgggttc caatatcatg gtcgtgtcca ggcactggca
     9781 gatgctgccc gtgaagctgg ccttcagttc taaggtagag gtgtaagatg gctcacatcg
     9841 aaaaacaagc tggcgaactg caggaaaagc tgatcgcggt aaaccgcgta tctaaaaccg
     9901 ttaaaggtgg tcgtattttc tccttcacag ctctgactgt agttggcgat ggtaacggtc
     9961 gcgttggttt tggttacggt aaagcgcgtg aagttccagc agcgatccag aaagcgatgg
    10021 aaaaagcccg tcgcaatatg attaacgtcg cgctgaataa cggcactctg caacaccctg
    10081 ttaaaggtgt tcacacgggt tctcgcgtat tcatgcagcc ggcttccgaa ggtaccggta
    10141 tcatcgccgg tggtgcaatg cgcgccgttc tggaagtcgc tggggttcat aacgttctgg
    10201 ctaaagcata tggttccacc aacccgatca acgtggttcg tgcaactatt gatggcctgg
    10261 aaaatatgaa ttctccagaa atggtcgctg ccaagcgtgg taaatccgtt gaagaaattc
    10321 tggggaaata aaccatggca aagactatta aaattactca aacccgcagt gcaatcggtc
    10381 gtctgccgaa acacaaggca acgctgcttg gcctgggtct gcgtcgtatt ggtcacaccg
    10441 tagagcgcga ggatactcct gctattcgcg gtatgatcaa cgcggtttcc ttcatggtta
    10501 aagttgagga gtaagagatg cgtttaaata ctctgtctcc ggccgaaggc tccaaaaagg
    10561 cgggtaaacg cctgggtcgt ggtatcggtt ctggcctcgg taaaaccggt ggtcgtggtc
    10621 acaaaggtca gaagtctcgt tctggcggtg gcgtacgtcg cggtttcgag ggtggtcaga
    10681 tgcctctgta ccgtcgtctg ccgaaattcg gcttcacttc tcgtaaagca gcgattacag
    10741 ccgaagttcg tctgtctgac ctggctaaag tagaaggcgg tgtagtagac ctgaacacgc
    10801 tgaaagcggc taacattatc ggtatccaga tcgagttcgc gaaagtgatc ctggctggcg
    10861 aagtaacgac tccggtaact gttcgtggcc tgcgtgttac taaaggcgct cgtgctgcta
    10921 tcgaagctgc tggcggtaaa atcgaggaat aagtagcaga tggctaaaca accgggatta
    10981 gattttcaaa gtgccaaagg tggcttaggc gagctgaaac gcagactgct gtttgttatc
    11041 ggtgcgctga ttgtgttccg tattggctct tttattccga tccctggtat tgatgccgct
    11101 gtacttgcca aactgcttga gcaacagcga ggcaccatca ttgagatgtt taacatgttc
    11161 tctggtggtg ctctcagccg tgcttctatc tttgctctgg ggatcatgcc gtatatttcg
    11221 gcgtcgatca ttatccagct gctgacggtg gttcacccaa cgttggcaga aattaagaaa
    11281 gaaggggagt ctggtcgtcg taagatcagc cagtacaccc gctacggtac tctggtgctg
    11341 gcaatattcc agtcgatcgg tattgctacc ggtctgccga atatgcctgg tatgcaaggc
    11401 ctggtgatta acccgggctt tgcattctac ttcaccgctg ttgtaagtct ggtcacagga
    11461 accatgttcc tgatgtggtt gggcgaacag attactgaac gaggtatcgg caacggtatt
    11521 tcaatcatta tcttcgccgg tattgtcgcg ggactcccgc cagccattgc ccatactatc
    11581 gagcaagcgc gtcaaggcga cctgcacttc ctcgtgttgc tgttggttgc agtattagta
    11641 tttgcagtga cgttctttgt tgtatttgtt gagcgtggtc aacgccgcat tgtggtaaac
    11701 tacgcgaaac gtcagcaagg tcgtcgtgtc tatgctgcac agagcacaca tttaccgctg
    11761 aaagtgaata tggcgggggt aatcccggca atcttcgctt ccagtattat tctgttcccg
    11821 gcgaccatcg cgtcatggtt cgggggcggt actggttgga actggctgac aacaatttcg
    11881 ctgtatttgc agcctgggca accgctttat gtgttactct atgcgtctgc aatcatcttc
    11941 ttctgtttct tctacacggc gttggttttc aacccgcgtg aaacagcaga taacctgaag
    12001 aagtccggtg catttgtacc aggaattcgt ccgggagagc aaacggcgaa gtatatcgat
    12061 aaagtaatga cccgcctgac cttggttggt gcgctgtaca ttacttttat ctgcctgatc
    12121 ccggagttca tgcgtgatgc aatgaaagta ccgttctact tcggtgggac ctcactgctt
    12181 atcgttgttg tcgtgattat ggactttatg gctcaagtgc aaactctgat gatgtccagt
    12241 cagtatgagt ctgcattgaa gaaggcgaac ctgaaaggct acggccgata attggtcgcc
    12301 cgagaagtta cggagagtaa aaatgaaagt tcgtgcttcc gtcaagaaat tatgccgtaa
    12361 ctgcaaaatc gttaagcgtg atggtgtcat ccgtgtgatt tgcagtgccg agccgaagca
    12421 taaacagcgc caaggctgat tttttcgcat atttttcttg caaagttggg ttgagctggc
    12481 tagattagcc agccaatctt ttgtatgtct gtgcgtttcc atttgagtat cctgaaaacg
    12541 ggcttttcag catggaacgt acatattaaa tagtaggagt gcatagtggc ccgtatagca
    12601 ggcattaaca ttcctgatca taagcatgcc gtaatcgcat taacttcgat ttatggcgtc
    12661 ggcaagaccc gttctaaagc catcctggct gcagcgggta tcgctgaaga tgttaagatc
    12721 agtgagctgt ctgaaggaca aatcgacacg ctgcgtgacg aagttgccaa atttgtcgtt
    12781 gaaggtgatc tgcgccgtga aatcagcatg agcatcaagc gcctgatgga tcttggttgc
    12841 tatcgcggtt tgcgtcatcg tcgtggtctc ccggttcgcg gtcagcgtac caagaccaac
    12901 gcacgtaccc gtaagggtcc gcgcaaaccg atcaagaaat aatcggggtg attgaataat
    12961 ggcaaaggca ccaattcgtg cacgtaaacg tgtaagaaaa caagtctctg acggcgtggc
    13021 tcatatccat gcttctttca acaacaccat cgtgactatc actgatcgtc agggtaacgc
    13081 gttgggttgg gcaacagccg gtggttccgg tttccgtggt tctcgcaaat ccactccgtt
    13141 tgcagctcag gttgcagcag agcgttgcgc tgacgccgtg aaagaatacg gcatcaagaa
    13201 tctggaagtt atggttaaag gtccgggtcc aggccgcgaa tctactattc gtgctctgaa
    13261 cgccgcaggt ttccgcatca ctaacattac tgatgtgact ccgatccctc ataacggttg
    13321 tcgtccgccg aaaaaacgtc gcgtataacg cctcgttttc caggtttgtt ggagaaagaa
    13381 aatggcaaga tatttgggtc ctaagctcaa gctgagccgt cgtgagggca ccgacttatt
    13441 ccttaagtct ggcgttcgcg cgatcgatac caagtgtaaa attgaacaag ctcctggcca
    13501 gcacggtgcg cgtaaaccgc gtctgtctga ctatggtgtg cagttgcgtg aaaagcaaaa
    13561 agttcgccgt atctatggtg tgctggagcg tcagttccgt aactactaca aagaagcagc
    13621 acgtctgaaa ggcaacaccg gtgaaaacct gttggctctg ctggaaggtc gtctggacaa
    13681 cgttgtatac cgtatgggct tcggtgccac tcgtgcagaa gcacgtcagc tggttagcca
    13741 taaagcaatt atggtaaacg gtcgtgttgt taacatcgct tcttatcagg ttagtccgaa
    13801 tgacgttgta agcattcgtg agaaagcgaa gaagcagtct cgcgtgaaag ccgctctgga
    13861 gctggctgag cagcgtgaaa agccaacctg gctggaagtt gatgctggca agatggaagg
    13921 tacgtttaag cgtaagccgg agcgttctga tctgtctgcg gacattaacg aacacctgat
    13981 cgtcgagctt tactccaagt aaagcttagt accaaagaga ggacacaatg cagggttctg
    14041 tgacagagtt tctaaaaccg cgcctggttg atatcgagca agtgagttcg acgcacgcca
    14101 aggtgaccct tgagccttta gagcgtggct ttggccatac tctgggtaac gcactgcgcc
    14161 gtattctgct ctcatcgatg ccgggttgcg cggtgaccga ggttgagatt gatggtgtac
    14221 tacatgagta cagcaccaaa gaaggcgttc aggaagatat cctggaaatc ctgctcaacc
    14281 tgaaagggct ggcggtgaga gttcagggca aagatgaagt tattcttacc ttgaataaat
    14341 ctggcattgg ccctgtgact gcagccgata tcacccacga cggtgatgtc gaaatcgtca
    14401 agccgcagca cgtgatctgc cacctgaccg atgagaacgc gtctattagc atgcgtatca
    14461 aagttcagcg cggtcgtggt tatgtgccgg cttctacccg aattcattcg gaagaagatg
    14521 agcgcccaat cggccgtctg ctggtcgacg catgctacag ccctgtggag cgtattgcct
    14581 acaatgttga agcagcgcgt gtagaacagc gtaccgacct ggacaagctg gtcatcgaaa
    14641 tggaaaccaa cggcacaatc gatcctgaag aggcgattcg tcgtgcggca accattctgg
    14701 ctgaacaact ggaagctttc gttgacttac gtgatgtacg tcagcctgaa gtgaaagaag
    14761 agaaaccaga gttcgatccg atcctgctgc gccctgttga cgatctggaa ttgactgtcc
    14821 gctctgctaa ctgccttaaa gcagaagcta tccactatat cggtgatctg gtacagcgta
    14881 ccgaggttga gctccttaaa acgcctaacc ttggtaaaaa atctcttact gagattaaag
    14941 acgtgctggc ttcccgtgga ctgtctctgg gcatgcgcct ggaaaactgg ccaccggcaa
    15001 gcatcgctga cgagtaaccg gatcacaggt taaggtttta ctgagaagga taaggtcatg
    15061 cgccatcgta agagtggtcg tcaactgaac cgcaacagca gccatcgcca ggctatgttc
    15121 cgcaatatgg caggttcact ggttcgtcat gaaatcatca agacgactct gcctaaagcg
    15181 aaagagctgc gccgcgtagt tgagccgctg attactcttg ccaagactga tagcgttgct
    15241 aatcgtcgtc tggcattcgc ccgtactcgt gataacgaga tcgtggcaaa actgtttaac
    15301 gaactgggcc cgcgtttcgc gagccgtgcc ggtggttaca ctcgtattct gaagtgtggc
    15361 ttccgtgcag gcgacaacgc gccgatggct tacatcgagc tggttgatcg ttcagagaaa
    15421 gcagaagctg ctgcagagta atctgaagca acgtaaaaaa acccgccccg gcgggttttt
    15481 ttatacccgt agtatcccca cttatctaca atagctgtac tctttttgtt catcccctgg
    15541 agtatttatg tggttacttg accagtgggc agagcgtcat attactgagg cacaatccaa
    15601 aggtgagttt gataatcttc cgggaagtgg cgagccattg attctggatg atgattcaca
    15661 cgtgccaccg gaattacgtg cggggtatcg cttgctgaag aatgccggtt gcttaccgcc
    15721 agaacttgag cagcgtaggg aagcaataca gcttctggat atcctcaaag gtatccgtca
    15781 tgatgatccg caatataaag aggttagtcg tcgattgtca ttactggaat tgaagctgcg
    15841 acaagctgga ttgagtaccg attttttacg cggcgattat gctgacaagt tgttggacaa
    15901 aatctacgat aactagtgga gtatgtatgt atcgcattgg tgagctggca aaaatggcgg
    15961 aagtaacacc cgacacgatt cgttactacg aaaagcagca gatgatggag catgaagtac
    16021 gcaccgaagg tgggtttcgc ctgtataccg aaagcgatct ccagcgattg aaatttatcc
    16081 gccatgccag acaacttggt ttcagtctgg agtcgatccg cgagttgctg tcgatccgta
    16141 tcgatcctga acaccatacc tgtcaggagt caaaaggcat tgtgcaggaa agattacagg
    16201 aagtcgaagc acggatagcc gagttgcaga gtatgcagcg ttccttgcaa cgccttaacg
    16261 atgcctgttg tgggaccgct catagcagtg tttattgctc gattcttgaa gctcttgaac
    16321 aaggggcgag tggcgttaag agtggctgtt gattttttgc tctggcagga ttacactcgc
    16381 gccgttaaat aaccaattgg agtttttatg agccgatatc agcatactaa agggcagata
    16441 aaggataatg cgatagaagc attactacat gatcccttat tccgacagcg cgtagagaga
    16501 aataagaaag ggaaaggcag ttacatgcga aaaggaaaac atggcaatcg ggggaactgg
    16561 gaggccagtg gcaagaaagt aaatcacttt tttaccactg gccttctgct ttcaggcgct
    16621 tgttaagagc ggttattctg ctctttcagc aaatcacgaa tttctgtcag taatacttct
    16681 tctttagttg gtgcaggcgc ggctgctggt tcttcttttt tccgattcag tttgttgatt
    16741 agcttaatcg ccataaagat ggcaaaggct acaatcagaa aatcaaagac gttttgaatg
    16801 aagacaccgt aatgcatcac aacagcaggg atatccccct gcgcatcgcg tagcgtgaca
    16861 gcaaactgtt tgaaatcgat cccgccaatt aataacccca gaggtggcat aatgatatcg
    16921 gcaaccagtg aagatacaat cttcccgaat gccgcaccga taatgacacc caccgccaaa
    16981 tccaccacgt tcccgcgcat cgcaaattcg cgaaattctt taataatgct catgttattc
    17041 tccctatgaa gccgacaatc ataagtctaa caaatgttaa gctattttcc tgccaggagt
    17101 gaatttaatt aatccctgat aatcaaaggg aaataataag gcgtcattag acgccttatt
    17161 aattacaaga agaaaggact tggctggaag agtctttcga cgtcggtaat aaacttttta
    17221 tctgtgagga acataattac gtgatcgcct tgctcaatgc gcagattgtc attggcaatc
    17281 atcacatcgt ttccacgtac caccgctcca ataatcgttc ctggcggtag cttgatttcg
    17341 tcaatgactc tgccgacaac gcgtgaggtg ctttcatcac cgtgagcaac ggcttcaata
    17401 gcttctgcta cgccgcggcg cagtgaggaa acaccaacaa tatcagcttt tcgcacatgg
    17461 ctaagcaacg cagaaatagt tgcttgttgc ggtgaaatcg caatatcgat cacgctcccc
    17521 tgaaccagat ccacataagc gcgacgctgg atcaatacca tcaccttttt cgcacccata
    17581 cgtttggcaa gcatggcgga catgatattg gcttcgtcat cgttggtgac agcaataaac
    17641 agatcaactt gatcgatatg ttcttcggcc agcagttctt gatccgacgc atcaccaaaa
    17701 aagacgatcg tattctgcaa cttttccgcc agttcggcag cgcgctgctg attacgttcg
    17761 atgagtttga cgctgtaatc tttttccaga cgacgcgcca gccctgcacc gatattacca
    17821 ccaccaacca gcatgatccg cttatacggt ttttccagtc gctgtaattc actcatcacc
    17881 gcgcggatat gctgtgaagc ggcaataaag aacacttcat caccagcttc aacaatggtc
    17941 gaaccttgcg gacgaatggg gcgatcatgg cggaaaattg ctgccacacg agtatcgata
    18001 tgtggcatat gttcgcgcat ggtcgaaagt gcattaccaa tcagcgggcc gccgtaatag
    18061 gctttaacca cagccaggct gactttaccc tcagcgaagt ttaccacctg caatgcgccg
    18121 ggatactcaa tcagtcggta aatattatcg ataaccaact gctctggtgc gatcagatga
    18181 tcaatcggta cagcatctga atgaaatagc ttatcggcat cgcgcacgta gtctggtgag
    18241 cggatacgag cgatgcgatt aggggtgttg aaaagtgagt aggctacctg gcaggcaacc
    18301 atattggttt catctgaact ggttacagca accagcatat cggcgtcgtc ggcacctgcc
    18361 tcccgcaata cgcgtggatg agagccatgc ccctgcacga ctcgcaggtc aaatttatcc
    18421 tgcaaggtcc gcagacgctc accgttggta tcgacaacag tgatatcgtt gttctcgcca
    18481 accaggtttt ccgccagtgt gccgccaacc tggccggcac ccagaatgat aattttcatc
    18541 agtcgcgacc cgttatctca tcactttttg attagcttag cgtaaaagaa gccgtcgccc
    18601 tcttcggcac caggtagatt ttgtttaccc ggttgctctg gtgttcctgt ttcgcaaagt
    18661 tcggcatcag cagtacgttg caaaaaggct ttaatctgca ggctattctc ttccggtaac
    18721 accgaacagg tggcatagac cagggttcca ccggatttta aatgcggcca aatggcgtcg
    18781 agaatttcag actgcaattg cgcgagttcc gggatatcgc gatcgcgacg taaccattta
    18841 atatccggat ggcgacgaat cacaccggtt gctgaacaag gcgcatctaa taaaatgcga
    18901 tcaaactgtt gctcgccaca ccattgggaa gggtaacggc catcaccttg tttcacggtt
    18961 gccttcatac caaggcgttt taaattgtcg taaacgcgag agaggcgctg ttcgtcaata
    19021 tcaaccgcaa caacctgcgc ttctggtgcc acctcaagga tatgcgttgt tttaccgccg
    19081 ggggcggcac aaagatccaa aatgtgttca ccgttttgtg gcgcaagcca ggtcatgcaa
    19141 ccttgtgctg aggcatcctg aacggtaacc catccgtctt caaaaccagg tagcgcatga
    19201 acaggtgcag gtgtttccag acgtacagca tcagggtaat ccgcatgcgg gaaacctttc
    19261 attcctgctt catccagcaa tgcaagccac ctgtcgcggg aatgatgcgt acgattaacg
    19321 cgcagccaca ttggcggacg ctggttattg gcttcgacga tggattgcca ctgctctgga
    19381 tacgcttttt gcagacgctt cagcaaccag gaaggatgca gataacgtgc atcactggca
    19441 ttaaactcgg ctaataactc ttcttgctga cgctggaact ggcgtaatac accgtttatc
    19501 aaccctttaa gttgcgggcg cttaattgcg atagcgcctt caaccgtttc agccagcgcg
    19561 gcatgaggtg gaatgcgggt ataaagcagt tgatacaaac caaccataat caggtaatga
    19621 acagtacgct gtttgcctgt catcggacgg gccattagct tattaatcag ccagtctaac
    19681 tgtgaaagcg tacgcagtac gccaaagcac aactcttgaa gaagtgcttt gtctttatca
    19741 gaaacttttt gctggagcgg tggcagaatg ttgcttaatg attgcccttg ctcgacgact
    19801 tgttcaacgg cctgggccgc catgctacgt aaattacgtt gttttttcat aagtataaaa
    19861 atgcccggca agaccgggct tagaagagtg gactatcagg ccagacggtt gcccggaaca
    19921 aaccattccc gacgagaatt caggaggtct tgtgcgctca tcgctttctt acccgcaggt
    19981 tgcaacgaga gcagattcag gatgccatca ccggtcgcga cctgaatgcc ttgtttgctg
    20041 gcttcaagga ttgttcctgg cgcagacttt gtcaccgtat caatgaccga cgctttccag
    20101 accttaacag gctggccttc aatttccagc cagctcattg gccatggatt gaaagcgcga
    20161 atgcagcgtt caagctgtgc tgccgaaagt gaccagtcaa tacgcgcttc ttctttactc
    20221 aacttctctg cgtaagtgac aagagtttcg tcctgaactt ctggtttcgc cgtgccgtct
    20281 gccaattgtt tcaatgtggt gataagccct tgtgggccaa gctctgccag cttgtcgtac
    20341 agcgtaccac tggtatcttc tgcagtaatc gggctggaga gcttatagag catgtcaccg
    20401 gtgtctaaac caacatccat ttgcataatg gtcacaccag tttctgcatc acccgcccat
    20461 agtgagcgtt ggattggtgc agcaccgcgc cagcgtggca gcagtgaacc atgaacgttg
    20521 atacagccaa gacgcggcat ctccagcacg gctttaggca gaattaagcc ataggcgacg
    20581 acaaccataa catcagcctg tagatcggcg accagttgct ggttttcttg tggacgcagg
    20641 gaaaccggtt gaaaaacggg cagacctttt tcctcagcca gaactttaac cgggctgggc
    20701 atcagttttt taccgcgtcc tgccggtcgg tctggctggg tgaacacgcc aacgacgtta
    20761 tgaccagaag acaacagcgc gtcgagatga cgcgctgcaa agtcaggtgt acccgcaaaa
    20821 ataatacgta gtgattctga cacgttagtt cttatcctta agcccgggct ttcagacgat
    20881 ccagtttttc aactttctga cgaatacgtt gttgtttcag cggtgacaga taatccataa
    20941 acagtttgcc gaccaggtga tccatctcat gctgaataca gatggctaac agaccctctg
    21001 cttccagttc aaatggttta ccgtcgcggt ccagggcgcg aattttaact ttctctgcgc
    21061 gcggcactaa agcacgttgt tcagggatcg acaggcatcc ttcttcaatg cctgtttcgc
    21121 cgcttttttc taaaagctcc ggattgatta acaccagccg ttcgtcacgg ttttccgaaa
    21181 catcaataac aatgatacgt tgatggatat cgacctgggt tgccgccagg ccaatacctt
    21241 cttctgcgta catcgtctcg aacatatcat cgacgatacg ctgaatttct gcattcactt
    21301 cttctaccgg tttagcaact ttgcgaagcc gctcgtccgg aatatgtaac acttgcaaaa
    21361 ctgacataaa tctccagaga tgtgttcagg agttagaaag attatttctt ctattctaga
    21421 caaatccccc tctgattgac agcatcactg accaatcgca aagattgcta aggctgctta
    21481 tggcagggag ataaggatgg tcgatacaga aatttggctg cgtttaatga gcatcagcag
    21541 cttgtacggc gatgatatgg tccgtatagc tcactggctg gcaaaacagt cgcatattga
    21601 tgcggttgta ttgcagcaaa ctgggcttac attgcggcag gcacaacgct ttctttcatt
    21661 tccgcgaaag agtatcgaaa gctcactttc ttggttggag caacccaacc atcatttaat
    21721 tcctgcggac agcgaatttt atcctcctca acttcttgcg acgacagatt accccggcgc
    21781 actgtttgtt gaaggagaac tgcacgcgct gcattcattt cagcttgccg tagtggggag
    21841 tcgggcgcat tcatggtatg gcgaacgctg gggacgatta ttttgcgaaa ctctggcgac
    21901 gcgtggagtg acaattacga gtggactggc gcgtggaatc gatggtgtgg cgcataaagc
    21961 ggccttacag gtaaatggcg tcagcattgc tgtattaggg aatggactta ataccattca
    22021 tccgcgtcgc catgcccgac tggctaccag tttgcttgaa catggtgggg ctcttgtctc
    22081 ggaatttccc ctcgatgttc ctccccttgc ttacaatttc ccacgaagaa atcgcattat
    22141 cagtggtcta agtaaaggtg tactggtggt ggaagcggct ttgcgcagtg gttcgctggt
    22201 gacagcacgt tgtgcgcttg agcaggggcg tgaagttttt gccttgccgg gtccattagg
    22261 gaatccggga agcgaagggc ctcactggtt aataaaacaa ggtgcgattc ttgtgacgga
    22321 accggaagaa attctggaaa acttgcaatt tggattgcac tggttgccag acgcccctga
    22381 aaattcattt tattcaccag atcaggaaga cgtggcattg ccatttcctg agctcctggc
    22441 taacgtagga gatgaggtaa cacctgttga cgtcgtcgct gaacgtgccg gccaacctgt
    22501 gccagaggta gtaactcaac tactcgaact ggagttagca ggatggatcg cagctgtacc
    22561 cggcggctat gtccgattga ggagggcatg ccatgttcga cgtactaatg tatttgtttg
    22621 aaacctatat tcacacagaa gctgagttgc gtgtggatca agacaaactt gaacaggatc
    22681 ttaccgacgc aggatttgat cgagaagata tctacaatgc cctgctttgg ctggaaaagc
    22741 ttgcggatta tcaggaagga ctggcagaac cgatgcaact ggcctctgat ccactctcca
    22801 tgcgtattta tacccctgaa gagtgtgaaa ggctggatgc cagctgtcgt ggttttctgc
    22861 ttttccttga gcaaattcag gtgcttaacc ttgaaacccg tgaaatggtg atagagcgag
    22921 tgctcgcgct ggataccgca gagttcgacc tggaagacct gaaatgggta atcctgatgg
    22981 tgttgttcaa tattccgggc tgcgaaaatg cgtaccagca aatggaagaa ttactctttg
    23041 aagtgaatga aggtatgctg cattaatcat ttctttaatt cagcataagt tgttatggcg
    23101 aaatcagcac tgttcacggt gcgtaataat gagtcctgcc caaagtgcgg ggctgaactg
    23161 gttattcgat ccgggaaaca cggtccgttt cttggatgct cacagtatcc ggcgtgtgac
    23221 tacgtccgtc ctctgaaatc ttcagcggat ggacatattg tcaaagttct ggaggggcag
    23281 gtttgccctg catgtggcgc acatctggta ttacgccagg gacgctttgg tatgtttatt
    23341 ggttgcagta actaccctga atgcgaacat accgaactta tcgataaacc ggacgaaaca
    23401 gcaattacat gcccccaatg ccggacgggc catctggtcc agcgccgcac ccgttatggc
    23461 aaaacgtttc actcctgtga tcgctacccg gagtgtcaat ttgccattaa cttcaaaccc
    23521 atagctggag aatgccctga gtgtcattat ccgctactca tcgaaaagaa aaccgcgcag
    23581 ggtgtaaaac acttttgtgc cagtaaacaa tgtggaaagc cggtttcggc ggaataataa
    23641 cgtgaataat aacctgcaag gagacgctat cgcagctgcg atagatgtcc tcaatgaaga
    23701 acgtgtcatc gcctatccaa cggaagccgt tttcggtgtc gggtgcgatc ctgatagcga
    23761 aacagcagtg atgcgactgt tggagttaaa acagcgtccg gttgataagg ggctgattct
    23821 aatcgccgca aattacgagc agcttaaacc ctatattgat gacaccatgc tgactgacgc
    23881 gcagcgtgaa actatatttt ctcgctggcc aggtcctgtc acctttgttt ttcccgcgcc
    23941 tgcgacaaca ccgcgctggt tgacgggccg ctttgattcg cttgctgtac gagtcaccga
    24001 ccatccgttg gtggttgctt tgtgccaggc ttatggtaaa ccgctggttt ctaccagtgc
    24061 caacttgagt ggattgccgc cttgtcgaac agtagacgaa gttcgcgcac aatttggcgc
    24121 ggcgttcccg gttgtgcctg gtgaaacggg ggggcgttta aatccttcag aaatccgcga
    24181 tgccctgacg ggtgaactgt ttcgacaggg gtaacataat ggaaacctat gctgtttttg
    24241 gtaatccgat agcccacagc aaatcgccat tcattcatca gcaatttgct cagcaactga
    24301 atattgaaca tccctatggg cgcgtgttgg cacccatcaa tgatttcatc aacacactga
    24361 acgctttctt tagtgctggt ggtaaaggtg cgaatgtgac ggtacctttt aaagaagagg
    24421 cttttgccag agcggatgag cttactgaac gggcaacgtt ggctggtgct gttaataccc
    24481 tcaagcggtt agaagatgga cgcctgctgg gtgacaatac cgatggtata ggtttgttaa
    24541 gcgatctgga acgtctgtct tttatccgtc ctggtttacg tattctgctc attggcgctg
    24601 gtggagcatc tcgcggcgta ctactgccac tcctttccct ggactgtgcg gtgacaatca
    24661 ctaatcggac ggtatcccgc gcggaagagt tggctaaatt gtttgcgcac actggcagta
    24721 ttcacgcgtt gggtatggac gaactggaag gtcatgagtt cgatctcatt attaatgcaa
    24781 catccagtgg catcagtggt gatattcccg cgatcccggc atcgctcatt cattcaggca
    24841 tgtgttgcta tgacatgttc tatcagaaag gaaaaacacc ttttctggca tggtgtgagc
    24901 tgcgaggttc aaagcgtaat gcggatggat tggggatgct ggtggcacag gcggctcatg
    24961 cctttcttct ctggcacggt gttctgcctg acatagaacc agttataaag caattgcagg
    25021 aggagttgtc cgcgtgaatc aggccatcca gtttccggac agggaagagt ggaacgagag
    25081 taaaaaatgc gtttgttttc ccgctcttgt aaatggtatg cagctcactt gcgctattac
    25141 tggagagagt ctggcgaacc gctttgcagg agatacgcca gaacagtggt tagcgagttt
    25201 tcgtcagcat cgctgggacc tggaagaaga agcggaaaac ttaattcagg aacaaagtga
    25261 agatgatcaa ggttgggtgt ggttaccctg atccatatat tcgtccttcc atttcacgta
    25321 gttatttgcg gaatagcgta acccagcctt ctcttcatca cttaacgggc ggatctgttt
    25381 gacggggcta ccgagataca gatatccgct ctccagccgt ttattttgtg ggaccaggct
    25441 acccgcacca atcatcacat catcttctac tattgcgcca tcaagtaaaa ttgagcccat
    25501 cccaaccaaa actcgattgc caatggtgca gccgtggagc atcaccttgt gaccaacagt
    25561 gacatcttcg ccaatggtta atgggttgcc agctgggttg tacgaggatt tatgagtgac
    25621 atgcaacata ctgccatcct ggatattggt gcgtgctccg atctgtacat aatgtacatc
    25681 tccacgaatc acaacgagcg gccagatccc cacatcatca gccagacgaa cgtcaccaat
    25741 cacgacgctg ctatcgtcga tcattacgcg ctgaccgatt tgtggaaaaa gatcgcggta
    25801 tgggcgtaaa acatcagaca tactcacctc aacaataaat gatttcctaa tgactttggg
    25861 tgtattattg gccttgtgca agtcttttag catgcaaaaa agcaccgttt tgtgtgcgaa
    25921 tgcagcaaaa agggtgaaaa agcaacaaac agaaaaaaag atcaaaaaag tacttgtgca
    25981 aaaaattggg atccctataa tgcgcctccg ttgagacgac aacgtgaaac acttcacaag
    26041 atggtcgtaa caacaaagag aaaaaatcct gaaattcagg gttgactctg aaagaggaaa
    26101 gcgtaatata cgccacctc
//
LOCUS       DAMZFT010000066        25993 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.066, whole genome shotgun sequence.
ACCESSION   DAMZFT010000066 DAMZFT010000000
VERSION     DAMZFT010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 25993)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25993)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25993
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.066"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..281
                     /gene="trpR"
                     /locus_tag="PN977_002802"
     CDS             <1..281
                     /gene="trpR"
                     /locus_tag="PN977_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="trp operon repressor"
                     /protein_id="HDI6042820.1"
                     /translation="QEWLRFVDLLKNAYQNDLHLPLLNLMLTPDEREALGTRVRIVEE
                     LLRGEMSQRELKNELGAGIATITRGSNSLKAAPVELRQWLEDVLLKDH"
     gene            complement(275..790)
                     /gene="yjjX"
                     /locus_tag="PN977_002803"
     CDS             complement(275..790)
                     /gene="yjjX"
                     /locus_tag="PN977_002803"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001649724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/xanthosine triphosphatase"
                     /protein_id="HDI6042821.1"
                     /translation="MHQVVCATTNPAKIQAILQAFHEIFGEGSCHIASVAVESGVPEQ
                     PFGSEETRAGARNRVANARRLLPEADFWVAIEAGIDGDSTFSWVVIENASQRGEARSA
                     TLPLPAVILEKVREGEALGPVMSRYTGIDEIGRKEGAIGVFTAGKLTRASVYHQAVIL
                     ALSPFHNAVYQ"
     gene            842..1489
                     /gene="gpmB"
                     /locus_tag="PN977_002804"
     CDS             842..1489
                     /gene="gpmB"
                     /locus_tag="PN977_002804"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase GpmB"
                     /protein_id="HDI6042822.1"
                     /translation="MLQVYLVRHGETQWNAERRIQGQSDSPLTAKGEQQAMQVATRAK
                     ELGITHIISSDLGRTRRTAEIIAQACGCDIIFDSRLRELNMGVLEKRHIDSLTEEEEN
                     WRRQLVNGTVDGRIPEGESMQELSDRVNAALESCRDLPQGSRPLLVSHGIALGCLVST
                     ILGLPAWAERRLRLRNCSISRVDYQESLWLASGWVVETAGDISHLDAPALDELQR"
     gene            complement(1486..2355)
                     /gene="robA"
                     /locus_tag="PN977_002805"
     CDS             complement(1486..2355)
                     /gene="robA"
                     /locus_tag="PN977_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     RobA"
                     /protein_id="HDI6042823.1"
                     /translation="MDQAGIIRDLLIWLEGHLDQPLSLDNVAAKAGYSKWHLQRMFKD
                     VTGHAIGAYIRARRLSKSAVALRLTARPILDIALQYRFDSQQTFTRAFKKQFAQTPAL
                     YRRSPEWSAFGIRPPLRLGEFTMPEHKFVTLEDTPLIGVTQSYSCSLEQISDFRHEMR
                     YQFWHDFLGNAPTIPPVLYGLNETRPSQDKDDEQEVFYTTALAQDQADGYVLTGHPVM
                     LQGGEYVMFTYEGLGTGVQEFILTVYGTCMPMLNLTRRKGQDIERYYPAEDAKAGDRP
                     INLRCELLIPIRR"
     gene            2566..3039
                     /gene="creA"
                     /locus_tag="PN977_002806"
     CDS             2566..3039
                     /gene="creA"
                     /locus_tag="PN977_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein CreA"
                     /protein_id="HDI6042824.1"
                     /translation="MKYKHLILSLSLIMLGPLAHAEEIGSVDTVFKMIGPDHKIVVEA
                     FDDPDVKNVTCYVSRAKTGGIKGGLGLAEDTSDAAISCQQVGPIELSDRIKNGKAQGE
                     VVFKKRTSLVFKSLQVVRFYDAKRNALAYLAYSDKVVEGSPKNAISAVPVMPWRQ"
     gene            3052..3741
                     /gene="creB"
                     /locus_tag="PN977_002807"
     CDS             3052..3741
                     /gene="creB"
                     /locus_tag="PN977_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator CreB"
                     /protein_id="HDI6042825.1"
                     /translation="MRRETVWLVEDEQGIADTLVYMLQQEGFAVEVFERGLPVLDKAR
                     QQVPDVMILDVGLPDISGFELCRQLLALHPALPVLFLTARSEEVDRLLGLEIGADDYV
                     AKPFSPREVCARVRTLLRRVKKFSTPSPVIRIGHFELNEPAAQISWFDTPLTLTRYEF
                     LLLKTLLKSPGRVWSRQQLMDSVWEDAQDTYDRTVDTHIKTLRAKLRAINPDLSPINT
                     HRGMGYSLRGL"
     gene            3741..5165
                     /gene="creC"
                     /locus_tag="PN977_002808"
     CDS             3741..5165
                     /gene="creC"
                     /locus_tag="PN977_002808"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     CreC"
                     /protein_id="HDI6042826.1"
                     /translation="MRIGMRLLLGYFLLVAVAAWFVLAIFVKEVKPGVRRATEGTLID
                     TATLLAELARPDLLSGDPTHGQLAQAFNQLQHRPFRANIGGINKVRNEYHVYMTDAQG
                     KVLFDSANKAVGQDYSRWNDVWLTLRGQYGARSTLQNPADPESSVMYVAAPIMDGSRL
                     IGVLSVGKPNAAMAPVIKRSERRILWASAILLGIALVIGAGMVWWINRSIARLTRYAD
                     SVTDNKPVPLPDLGSSELRKLAQALESMRVKLEGKNYIEQYVYALTHELKSPLAAIRG
                     AAEILREGPPPEVVARFTDNILTQNARMQALVETLLRQARLENRQEVVLTVVDVAALF
                     RRVSEARTVQLAEKKITLHVMPTEVNVAAEPTLLEQALGNLLDNAIDFTPESGRITLS
                     AEVEQEHVTLKVLDTGSGIPDYALSRIFERFYSLPRANGQKSSGLGLAFVSEVARLFN
                     GEVTLHNVQEGGVLASLRLHRHFT"
     gene            5223..6575
                     /gene="creD"
                     /locus_tag="PN977_002809"
     CDS             5223..6575
                     /gene="creD"
                     /locus_tag="PN977_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein CreD"
                     /protein_id="HDI6042827.1"
                     /translation="MLKSPLFWKMTTLFGAVLLLLIPIMLIRQVIVERADYRSDVEDA
                     IRQSTSGPQKLVGPLIAIPVTELYTVQEEDKTVERKRSFIHFWLPESLMVDGNQNVEE
                     RKIGIYTGQVWHSDLTLKADFDVSRLSELDAPNITFGKPFIVISVGDARGIGVVKAPE
                     VNGTALTIEPGTGLEQGGQGVHIPLPEGDWRKQNLKLNMALNLSGTGDLSVVPAGRNS
                     EMTLTSNWPHPSFLGDFLPAKREVSESGFQAQWQSSWFANNLGERFASGNDTGWENFP
                     AFSVAVTTPADQYQLTDRATKYAILLIALTFMAFFVFETLTAQRLHPMQYLLVGLSLV
                     MFYLLLLALSEHTGFTVAWIIASLIGALMNGIYLQAVLKGWRNSMLFTLALLLLDGVM
                     WGLLNSADSALLLGTSVLVVALAGMMFVTRNIDWYAFSLPKMKASKEVTTDDELRIWK
                     "
     gene            complement(6634..7350)
                     /gene="arcA"
                     /locus_tag="PN977_002810"
     CDS             complement(6634..7350)
                     /gene="arcA"
                     /locus_tag="PN977_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003621071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator ArcA"
                     /protein_id="HDI6042828.1"
                     /translation="MQTPHILIVEDELVTRNTLKSIFEAEGYDVFEATDGAEMHQILS
                     EYDINLVIMDINLPGKNGLLLARELREQANVALMFLTGRDNEVDKILGLEIGADDYIT
                     KPFNPRELTIRARNLLSRTMNLGTVSEERRSVESYKFNGWELDINSRSLIGPDGEQYK
                     LPRSEFRAMLHFCENPGKIQSRAELLKKMTGRELKPHDRTVDVTIRRIRKHFESTPDT
                     PEIIATIHGEGYRFCGDLED"
     gene            7446..7586
                     /locus_tag="PN977_002811"
     CDS             7446..7586
                     /locus_tag="PN977_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6042829.1"
                     /translation="MTKVRNCVLDALSINVNNIISLVVGTFPQDPTVSKTAVILTILT
                     AT"
     gene            7986..8672
                     /locus_tag="PN977_002812"
     CDS             7986..8672
                     /locus_tag="PN977_002812"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HDI6042830.1"
                     /translation="MRITIILVAPARAENIGAAARAMKTMGFSDLRIVDSQAHLEPAT
                     RWVAHGSSDIIDNIKVFPTLAESLHDVDFTVATTARSRAKYHYYATPVELVPLLEEKS
                     SWMSHAALVFGREDSGLTNEELALADVLTGVPMVADYPSLNLGQAVMVYCYQLATLIQ
                     QPAKSDATADQHQLQALRERAMTLLTTLAVADDIKLVDWLQQRLGLLEQRDTAMLHRL
                     LHDIEKNITK"
     gene            8886..8951
                     /gene="thrL"
                     /locus_tag="PN977_002813"
     CDS             8886..8951
                     /gene="thrL"
                     /locus_tag="PN977_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thr operon leader peptide"
                     /protein_id="HDI6042831.1"
                     /translation="MKRISTTITTTITITTGNGAG"
     misc_feature    8887..9006
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00506"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Thr leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00506"
     gene            9033..11495
                     /gene="thrA"
                     /locus_tag="PN977_002814"
     CDS             9033..11495
                     /gene="thrA"
                     /locus_tag="PN977_002814"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase I"
                     /protein_id="HDI6042832.1"
                     /translation="MRVLKFGGTSVANAERFLRVADILESNARQGQVATVLSAPAKIT
                     NHLVAMIEKTISGQDALPNISDAERIFAELLTGLAAAQPGFPLAQLKTFVDQEFAQIK
                     HVLHGISLLGQCPDSINAALICRGEKMSIAIMAGVLEARGHNVTVIDPVEKLLAVGHY
                     LESTVDIAESTRRIAASRIPADHMVLMAGFTAGNEKGELVVLGRNGSDYSAAVLAACL
                     RADCCEIWTDVDGVYTCDPRQVPDARLLKSMSYQEAMELSYFGAKVLHPRTITPIAQF
                     QIPCLIKNTGNPQAPGTLIGASRDEDELPVKGISNLNNMAMFSVSGPGMKGMVGMAAR
                     VFAAMSRARISVVLITQSSSEYSISFCVPQSDCVRAERAMQEEFYLELKEGLLEPLAV
                     TERLAIISVVGDGMRTLRGISAKFFAALARANINIVAIAQGSSERSISVVVNNDDATT
                     GVRVTHQMLFNTDQVIEVFVIGVGGVGGALLEQLKRQQSWLKNKHIDLRVCGVANSKA
                     LLTNVHGLNLENWQEELAQAKEPFNLGRLIRLVKEYHLLNPVIVDCTSSQAVADQYAD
                     FLREGFHVVTPNKKANTSSMDYYHQLRYAAEKSRRKFLYDTNVGAGLPVIENLQNLLN
                     AGDELMKFSGILSGSLSYIFGKLDEGMSFSEATTLAREMGYTEPDPRDDLSGMDVARK
                     LLILARETGRELELADIEIEPVLPAEFNAEGDVAAFMANLSQLDDLFAARVAKARDEG
                     KVLRYVGNIDEDGICRVKIAEVDGNDPLFKVKNGENALAFYSHYYQPLPLVLRGYGAG
                     NDVTAAGVFADLLRTLSWKLGV"
     gene            11497..12429
                     /gene="thrB"
                     /locus_tag="PN977_002815"
     CDS             11497..12429
                     /gene="thrB"
                     /locus_tag="PN977_002815"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HDI6042833.1"
                     /translation="MVKVYAPASSANMSVGFDVLGAAVTPVDGALLGDVVTVEAAETF
                     SLNNLGRFADKLPSEPRENIVYQCWERFCQELGKQIPVAMTLEKNMPIGSGLGSSACS
                     VVAALMAMNEHCGKPLNDTRLLALMGELEGRISGSIHYDNVAPCFLGGMQLMIEENDI
                     ISQQVPGFDEWLWVLAYPGIKVSTAEARAILPAQYRRQDCIAHGRHLAGFIHACYSRQ
                     PELAAKLMKDVIAEPYRERLLPGFRQARQAVAEIGAVASGISGSGPTLFALCDKPETA
                     QRVADWLGKNYLQNQEGFVHICQLDTAGARVLEN"
     gene            12430..13716
                     /gene="thrC"
                     /locus_tag="PN977_002816"
     CDS             12430..13716
                     /gene="thrC"
                     /locus_tag="PN977_002816"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HDI6042834.1"
                     /translation="MKLYNLKDHNEQVSFAQAVTQGLGKNQGLFFPHDLPEFSLTEID
                     EMLKLDFVTRSAKILSAFIGDEIPQEILEERVRAAFAFPAPVANVESDVGCLELFHGP
                     TLAFKDFGGRFMAQMLTHIAGDKPVTILTATSGDTGAAVAHAFYGLPNVKVVILYPRG
                     KISPLQEKLFCTLGGNIETVAIDGDFDACQALVKQAFDDEELKVALGLNSANSINISR
                     LLAQICYYFEAVAQLPQEARNQLVVSVPSGNFGDLTAGLLAKSLGLPVKRFIAATNVN
                     DTVPRFLHDGQWSPKATQATLSNAMDVSQPNNWPRVEELFRRKIWQLKELGYAAVDDE
                     TTQQTMRELKELGYTSEPHAAVAYRALRDQLHPGEYGLFLGTAHPAKFKESVEAILGE
                     TLDLPKELAERADLPLLSHNLPADFAALRKLMMNHQ"
     gene            13930..14226
                     /locus_tag="PN977_002817"
     CDS             13930..14226
                     /locus_tag="PN977_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein"
                     /protein_id="HDI6042835.1"
                     /translation="MKKMQSIVLALSLVLVAPMAAQAAEITLVPSVKLQIGDRDNRGY
                     YWDGGHWRDHGWWKQHYEWRGNRWHPHGPPPSPRHNKHNDHRGDHRPGPDKHHR"
     gene            complement(14266..15042)
                     /gene="yaaA"
                     /locus_tag="PN977_002818"
     CDS             complement(14266..15042)
                     /gene="yaaA"
                     /locus_tag="PN977_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HDI6042836.1"
                     /translation="MLILISPAKTLDYQSPLPTTRYTLPELLDNSQQLIHEARKLTPP
                     QISSLMRISDKLAGINAARFHDWQPDFTSENARQAILAFKGDVYTGLQAETFSEDDFD
                     FAQQHLRMLSGLYGVLRPLDLMQPYRLEMGIRLENARGKDLYQFWGDIITNKLNEALA
                     AQGDNVVINLASDEYFKSVKPKKLNAEIIKPVFLDEKNGKFKIISFYAKKARGLMSRF
                     IIENRLTKPEQLTGFNSEGYFFDEASSSNGELVFKRYEQR"
     gene            complement(15112..16542)
                     /locus_tag="PN977_002819"
     CDS             complement(15112..16542)
                     /locus_tag="PN977_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
                     /protein_id="HDI6042837.1"
                     /translation="MPDFFSFINSVLWGSVMIYLLFGAGCWFTFRTGFVQFRYIRQFG
                     KSLKNSIHPQPGGLTSFQSLCTSLAARVGSGNLAGVALAITAGGPGAVFWMWVAAFIG
                     MATSFAECSLAQLYKERDVNGQFRGGPAWYMARGLGMRWMGVLFALFLLIAYGIIFSG
                     VQANAVARALSFSFDFPPLVTGIILAVFALLAITRGLHGVARLMQGFVPLMAIIWVLT
                     SLVICVMNIGQLPHVIWSIFESAFGWQEAAGGAAGYTLSQAITNGFQRSMFSNEAGMG
                     STPNAAAAAASWPPHPAAQGIVQMIGIFIDTLVICTASAMLILLAGNGTTYMPLEGIQ
                     LIQKAMRVLMGSWGAEFVTLVVILFAFSSIVANYIYAENNLFFLRLNNPKAIWCLRIC
                     TFATVIGGTLLSLPLMWQLADIIMACMAITNLTAILLLSPVVHTIASDYLRQRKLGVR
                     PVFDPLRYPEIGRQLSPDAWDDVSQE"
     gene            16821..17774
                     /gene="tal"
                     /locus_tag="PN977_002820"
     CDS             16821..17774
                     /gene="tal"
                     /locus_tag="PN977_002820"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HDI6042838.1"
                     /translation="MTDKLTSLRQYTTVVADTGDIAAMKLYQPQDATTNPSLILNAAQ
                     IPEYRKLIDDAVAWAKQQSNDRAQQIVDATDKLAVNIGLEILKLVPGRISTEVDARLS
                     YDTEASIAKAKRLIKLYNDAGISNDRILIKLASTWQGIRAAEQLEKEGINCNLTLLFS
                     FAQARACAEAGVFLISPFVGRILDWYKANTDKKEYAPAEDPGVVSVSEIYQYYKEHGY
                     ETVVMGASFRNIGEILELAGCDRLTIAPALLKELAESEGAIERKLSYTGEVKARPARI
                     TESEFLWQHNQDPMAVDKLAEGIRKFAVDQEKLEKMIGDLL"
     gene            17889..18476
                     /gene="mog"
                     /locus_tag="PN977_002821"
     CDS             17889..18476
                     /gene="mog"
                     /locus_tag="PN977_002821"
                     /EC_number="2.7.7.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin adenylyltransferase"
                     /protein_id="HDI6042839.1"
                     /translation="MNTLRIGLVSISDRASSGVYQDKGIPALEEWLTSALTTPFELET
                     RLIPDEQAIIEQTLCELVDEMSCHLVLTTGGTGPARRDVTPDATLAVADREMPGFGEQ
                     MRQISLHFVPTAILSRQVGVIRKQALILNLPGQPKSIKETLEGVKDAEGNVVVHGIFA
                     SVPYCIQLLEGPYVETAPEVVAAFRPKSARREVSE"
     gene            complement(18511..19077)
                     /gene="satP"
                     /locus_tag="PN977_002822"
     CDS             complement(18511..19077)
                     /gene="satP"
                     /locus_tag="PN977_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate uptake transporter"
                     /protein_id="HDI6042840.1"
                     /translation="MGNTKLANPAPLGLMGFGMTTILLNLHNVGYFALDGIILAMGIF
                     YGGIAQIFAGLLEYKKGNTFGLTAFTSYGSFWLTLVAILLMPKLGLTDAPNAQFLGVY
                     LGLWGVFTLFMFFGTLKGARVLQFVFFSLTVLFALLAIGNIVGNAAIIHFAGWIGLIC
                     GASAIYLAMGEVLNEQFGRTVLPIGESH"
     gene            complement(19226..19939)
                     /gene="msyB"
                     /locus_tag="PN977_002823"
     CDS             complement(19226..19939)
                     /gene="msyB"
                     /locus_tag="PN977_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acidic protein MsyB"
                     /protein_id="HDI6042841.1"
                     /translation="MNVNYLNDSDLDFLQHCSEEQLANFARLLTHNEKGKTRLSSVLM
                     RNELFKSMEGHPEQHRRNWQLIAGELQHFGGDSIANKLRGHGKLYRAILLDVSKRLKL
                     KADKEMSTFEIEQQLLEQFLRNTWKKMDEEHKQEFLHAVDARVNELEELLPLLMKDKL
                     LAKGVSHLLSSQLTRILRTHAAMSVLGHGLLRGAGLGGPVGAALNGVKAVSGSAYRVT
                     IPAVLQIACLRRMVSATQV"
     gene            complement(19964..20368)
                     /locus_tag="PN977_002824"
     CDS             complement(19964..20368)
                     /locus_tag="PN977_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2541 family protein"
                     /protein_id="HDI6042842.1"
                     /translation="MKSVFTLSASLAISLLLCCTAQANDHKILGVIAMPRNETNDLAL
                     KLPVCRIVKRIQLTADHGDLQLSGASVYFKAARSASQSLNIPSEIKEGQTTDWININS
                     DNDNKRCVSKITFSGHTVNSSDMATLKIIGDD"
     gene            20744..22660
                     /gene="dnaK"
                     /locus_tag="PN977_002825"
     CDS             20744..22660
                     /gene="dnaK"
                     /locus_tag="PN977_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001737966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HDI6042843.1"
                     /translation="MGKIIGIDLGTTNSCVAIMDGTTPRVLENAEGDRTTPSIIAYTQ
                     DGETLVGQPAKRQAVTNPQNTLFAIKRLIGRRFQDEEVQRDVSIMPFKIIAADNGDAW
                     VEVKGQKMAPPQISAEVLKKMKKTAEDYLGEPVTEAVITVPAYFNDAQRQATKDAGRI
                     AGLEVKRIINEPTAAALAYGLDKGTGNRTIAVYDLGGGTFDISIIEIDEVDGEKTFEV
                     LATNGDTHLGGEDFDSRLINYLVEEFKKDQGIDLRNDPLAMQRLKEAAEKAKIELSSA
                     QQTDVNLPYITADATGPKHMNIKVTRAKLESLVEDLVNRSIEPLKVALQDAGLSVSDI
                     DDVILVGGQTRMPMVQKKVAEFFGKEPRKDVNPDEAVAIGAAVQGGVLTGDVKDVLLL
                     DVTPLSLGIETMGGVMTTLIAKNTTIPTKHSQVFSTAEDNQSAVTIHVLQGERKRAAD
                     NKSLGQFNLDGINPAPRGMPQIEVTFDIDADGILHVSAKDKNSGKEQKITIKASSGLN
                     EDEIQKMVRDAEANAEADRKFEELVQTRNQGDHLLHSTRKQVEEAGDKLPADDKTAIE
                     SALTALETALKGEDKAAIEAKMQELAQVSQKLMEIAQQQHAQQQTAGADASANNAKDD
                     DVVDAEFEEVKDKK"
     gene            22749..23879
                     /gene="dnaJ"
                     /locus_tag="PN977_002826"
     CDS             22749..23879
                     /gene="dnaJ"
                     /locus_tag="PN977_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HDI6042844.1"
                     /translation="MAKQDYYEILGVSKTAEEREIKKAYKRLAMKYHPDRNQGDKEAE
                     AKFKEIKEAYEVLTDSQKRAAYDQYGHAAFEQGGMGGGGFGGGADFSDIFGDVFGDIF
                     GGGRGRQRAARGADLRYNMELTLEEAVRGVTKEIRIPTLEECDVCHGSGAKPGTQPQT
                     CPTCHGSGQVQMRQGFFAVQQTCPHCQGRGTLIKDPCNKCHGHGRVERSKTLSVKIPA
                     GVDTGDRIRLAGEGEAGEHGAPAGDLYVQVQVKQHPIFEREGNNLYCEVPINFAMAAL
                     GGEIEVPTLDGRVKLKVPGETQTGKLFRMRGKGVKSVRGGAQGDLLCRVVVETPVGLN
                     EKQKQLLQELQESFGGPTGEHNSPRSKSFFDGVKKFFDDLTR"
     gene            complement(23983..24135)
                     /gene="mokC"
                     /locus_tag="PN977_002827"
     CDS             complement(23983..24135)
                     /gene="mokC"
                     /locus_tag="PN977_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin MokC"
                     /protein_id="HDI6042845.1"
                     /translation="MKQHKAMIVALIVICITAVVAALVTRKDLCEVHIRTGQTEVAVF
                     TAYESE"
     gene            24747..25508
                     /locus_tag="PN977_002828"
     CDS             24747..25508
                     /locus_tag="PN977_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001526572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6042846.1"
                     /translation="MSAKRRLLIACALTTIFYHLPAYSSLEYKGSFGSINAGYADWNS
                     GFVNTHRGEVWKVTADFGVNFKEAEFYSFYESNVLNHAVAGRNHTVSAMTHVRLFDSN
                     MTFFGKIYGQWDNSWGDDLDMFYGLGYLGWNGSWGFFKPYIGLHNQSGDYVSAKYGQT
                     NGWNGYVVGWTAVLPFTLFDEKFVLSNWNEIELDRNDAYAEQQFGRNGLNGGLTVAWK
                     FYPRWKASVTWRYFDNKLGYDGFGDQMIYMLGYDF"
     gene            25528..>25993
                     /locus_tag="PN977_002829"
     CDS             25528..>25993
                     /locus_tag="PN977_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HDI6042847.1"
                     /translation="MQKTLMASLIGLAVCTGNAFNPAVAAETKQPNLVIIMADDLGYG
                     DLATYGHQIVKTPNIDRLAQEGVKFTDYYAPAPLSSPSRAGLLTGRMPFRTGIRSWIP
                     TGKDVALGRNELTIANLLKAQGYDTAMMGKLHLNAGGDRTDQPQAKDMGFDYS"
BASE COUNT         6553 a         6276 c         6886 g         6278 t
ORIGIN      
        1 accaggagtg gttacgtttt gtcgacctgc ttaagaatgc ctaccaaaac gatctccatt
       61 taccgttgtt aaacctgatg ctgacgccag atgagcgcga agcgttgggg actcgcgtgc
      121 gtattgtcga agagttgttg cgcggcgaaa tgagccagcg tgagttaaaa aatgaactcg
      181 gcgcgggcat cgcgacgatt acgcgtggat ctaacagcct gaaagccgcg cccgtcgagc
      241 tgcgacagtg gctggaagac gtgttgctga aagatcactg atacaccgca ttatgaaacg
      301 gactcagcgc caggatcacc gcctggtgat agacgctggc gcgagtgagt ttcccggcgg
      361 taaacacgcc gatcgcccct tccttacgac caatctcatc aataccggta taacgcgaca
      421 tcacgggacc aagtgcctca ccttcacgca ctttttccag aatcaccgca ggcaacggca
      481 aagtagccga acgcgcctcg ccgcgctggc tggcgttttc aatcaccacc caactgaaag
      541 tgctgtcacc atcgatgcca gcttcaatcg ccacccaaaa atcagcctct ggaagtaaac
      601 ggcgagcatt tgctacccga tttcgtgcgc cagcgcgcgt ttcctcactg ccaaagggtt
      661 gttccggtac accgctctcg acggcaacgg atgcaatatg gcaggatcct tcgccgaaga
      721 tctcgtgaaa tgcctgcaga atggcctgaa ttttagcggg attggtggtc gcacagacaa
      781 cttggtgcat aatcagcatt actcagaaaa ttaacgttac agcagtatac ggaaaaaaag
      841 catgttacag gtatacctag tccgccacgg tgaaacgcag tggaacgccg agcgacgtat
      901 tcagggccag tctgacagcc cgctgaccgc caaaggtgag caacaggcga tgcaggtggc
      961 aacccgtgcc aaagagcttg gcattacgca tatcatcagt agcgatttag gacgcacccg
     1021 gcgtacggcg gaaatcatcg cccaggcctg cggctgtgac atcatctttg attctcgcct
     1081 gcgtgaatta aacatggggg tgctggaaaa aagacatatc gattctctga ccgaagaaga
     1141 agagaactgg cgtcggcagc tggtcaatgg caccgttgac gggcgtattc ctgaaggaga
     1201 gtcaatgcag gagctcagcg atcgcgtcaa tgcggcactg gaatcttgcc gggacttacc
     1261 gcagggaagc cgaccgctgc tggtgagcca cggtattgca ctgggatgcc tggtgagtac
     1321 gattctcgga ttaccagcat gggcagagcg tcgcttacgt ctgcgtaact gttctatttc
     1381 gcgcgtggat tatcaggaaa gcctgtggct ggcgtctggc tgggtcgttg aaaccgcagg
     1441 ggacatctcg catctggacg cccctgcatt agatgagctg cagcgttaac gacggatcgg
     1501 aatcagcagt tcacagcgca gattaattgg gcgatctccc gctttggcat cttctgccgg
     1561 gtagtatcgc tcaatatcct gacctttacg gcgcgtcagg ttgagcattg gcatgcacgt
     1621 tccgtatacc gtcaggataa actcctgcac gccggttccc agaccttcat aggtaaacat
     1681 cacatattca ccgccttgca gcatcacagg atgcccggtc aatacatagc catctgcctg
     1741 atcctgggct aatgcggtgg tatagaacac ttcttgctcg tcgtctttat cctggctcgg
     1801 acgcgtttca ttcaggccgt agagcacagg tggaatggtc ggcgcgttgc cgagaaaatc
     1861 gtgccagaac tgataacgca tttcatggcg gaaatcagag atttgctcca gcgaacagga
     1921 gtagctttgg gttacaccaa tcagcggcgt atcttccagg gtgacaaatt tgtgctctgg
     1981 catagtgaat tcacccagac gtagcggcgg gcgaatacca aaggcgctcc attcaggaga
     2041 acggcggtaa agtgcaggag tctgggcaaa ctgcttcttg aatgcgcggg taaatgtctg
     2101 ttgagagtcg aagcggtatt gcagcgcgat gtccagaatc ggacgcgcag tcaggcgtag
     2161 tgcgaccgcc gatttcgaca aacgacgagc acgaatatac gcgccaatag catggccagt
     2221 gacatcttta aacattctct gtaagtgcca cttggaataa cctgctttcg ccgctacatt
     2281 gtcgagcgac aggggctgat ccagatgacc ttccagccag attaaaaggt cgcgaataat
     2341 gccggcctga tccataaaat atcctcatcc tttcaacaac gagcacctga catcaggtaa
     2401 ttggataata gcattttttg ctgttttagc attcagtgtt tttttcttag tagagtatgt
     2461 tttaggactt gacggaaata aaagtattga gattttgttc ttaatcaata tgttatttac
     2521 cgtgacgaac taattgctcg tgtaatagat aaaaatggta acaatatgaa atacaagcat
     2581 ttgatcctgt ctttaagcct gataatgctg gggccattgg ctcatgcaga agagattggt
     2641 tcggtcgaca ccgtatttaa aatgatcggc ccggatcaca aaattgttgt ggaagccttt
     2701 gatgatcccg atgtgaaaaa tgtcacctgt tatgtgagcc gggcgaaaac cggtggtatt
     2761 aaagggggat tgggtctggc ggaagatacc tccgatgcgg ccatttcttg tcagcaagtc
     2821 gggccgattg aactgtcgga tcgtattaaa aacggcaaag ctcagggcga ggtagtattc
     2881 aaaaaacgta cgtccctggt ctttaagtcg ttacaggtcg tgcgctttta tgacgctaaa
     2941 cgcaacgcgc tcgcttatct ggcttactcc gacaaagttg tagaaggttc gccgaaaaac
     3001 gcgattagcg cggttcctgt catgccgtgg cggcaataac agaggcgatt tatgcgacgg
     3061 gaaacggtct ggttagtgga agatgagcaa gggatagccg acacgctggt ctacatgttg
     3121 cagcaggaag gttttgctgt cgaggtcttt gagcgaggct tgccggtgct ggataaagct
     3181 cgccagcagg tacccgacgt catgattctc gatgttggtc tgccggatat cagcggcttt
     3241 gaattgtgcc gccagttact ggcgctccat ccggcgttac ctgtactgtt cctgacggcc
     3301 cgaagtgaag aggtcgatcg cctgcttggg ctggaaattg gcgctgacga ctatgtggct
     3361 aaaccgtttt caccccgcga agtgtgcgcc agggtgcgca ccttactgcg tcgggtgaag
     3421 aagttctcga cgccgtctcc cgtcatccgt attggtcatt ttgaattgaa tgaacccgcg
     3481 gcgcagatca gctggtttga cacgccatta acgctgacgc ggtatgagtt tttattgctg
     3541 aagacgttac tcaagtcgcc aggccgcgtc tggtcccgcc agcaactgat ggatagcgtg
     3601 tgggaagatg cgcaggacac ctacgatcgc accgtcgata cccacattaa aacgctgcgt
     3661 gccaagctgc gcgccatcaa ccccgatctt tcaccgatta atactcatcg cggcatggga
     3721 tatagcctga ggggcctgta atgcgtatcg gcatgcggtt gttgctgggc tattttttac
     3781 tggtggcggt ggcagcctgg ttcgtactgg ccatttttgt caaagaagtt aaaccgggcg
     3841 tgcgaagagc aacggagggg acgttgatcg acaccgcaac gttgctggcg gagctggcgc
     3901 gtcccgattt gctctctggg gacccaacgc atgggcaact ggcgcaggcg tttaatcagc
     3961 tacaacatcg cccgtttcgc gccaatatcg gtggcattaa caaagtgcgc aatgaatatc
     4021 atgtctatat gaccgatgcg cagggcaaag tattgttcga ttcggcaaat aaagccgttg
     4081 gacaggatta ttcgcgctgg aatgacgtct ggctaacgtt gcgtggtcag tatggtgcgc
     4141 gcagcacgtt gcaaaatcct gccgatcccg aaagttctgt gatgtatgtt gccgcaccga
     4201 ttatggacgg ctcgcggctt attggcgttt tgagcgtagg caaaccgaac gcggcgatgg
     4261 ctccggtcat taagcgtagc gagcggcgaa ttttatgggc cagcgccatt ttgttgggga
     4321 ttgcactggt gattggcgca ggcatggttt ggtggatcaa ccgctctatt gcccggctca
     4381 ctcgctatgc tgattccgtc actgacaata agcccgttcc tctccccgat ctcggtagta
     4441 gcgagttgcg taaactcgcg caggcgctgg aaagtatgcg cgtgaagctg gaagggaaaa
     4501 actatattga gcagtatgtt tatgcgttaa cccatgagct aaaaagccca ctggcggcga
     4561 ttcgtggcgc ggcggaaatt ttacgcgaag gtccgccgcc ggaagtggtg gctcgtttta
     4621 ctgacaacat tctgacgcaa aatgcgcgta tgcaggcact ggtggaaacg ttactacgcc
     4681 aggcaagact ggagaatcgt caggaagtcg ttctgactgt tgttgatgtg gcggcattat
     4741 tccgccgcgt cagcgaagcg cgcaccgtgc agttggcaga aaaaaaaatc actctgcatg
     4801 ttatgcccac cgaggttaac gttgctgctg aaccgacgtt actggagcag gcgctgggga
     4861 atttactgga taacgccatc gattttaccc ccgagagcgg tcgtataacg ctaagcgccg
     4921 aagtggagca ggaacacgtc acgcttaagg tgctggatac cggtagtggt attcctgact
     4981 acgcgctttc acgtattttt gaacgctttt actctttgcc gcgtgcaaat gggcaaaaaa
     5041 gcagcggtct ggggttggcg ttcgtcagtg aggtcgcccg tttgtttaac ggcgaagtca
     5101 cgctgcacaa cgtgcaggaa ggtggcgtgc tggcctcgct tcgacttcac cgtcacttca
     5161 catagcttca aattcttccc acatagcctt cgtatcctgc tgccattgca aaggagaaga
     5221 ctatgttgaa atcccccctg ttctggaaaa tgaccaccct gtttggtgcc gtattgctgt
     5281 tgttgattcc gataatgctg attcggcagg tgattgtcga acgtgccgat taccgtagcg
     5341 atgtggaaga tgcgattcgc caaagtacca gcgggccgca aaaactcgtt gggccgctca
     5401 tcgctattcc tgtgaccgag ctttatacgg tgcaggaaga ggataaaacc gtggagcgga
     5461 aacgaagttt tatccatttt tggttacctg agtcattgat ggttgatggc aatcagaacg
     5521 tggaagaacg caagataggg atttataccg gtcaggtctg gcacagtgat ttaacgctaa
     5581 aagccgattt cgatgtttcg cgtcttagcg aactcgacgc gccaaatatc acctttggca
     5641 agccatttat tgtgattagc gtcggggatg cgcgtggtat tggtgtggtg aaagcgcctg
     5701 aagttaacgg aacggcgctg accattgaac ccggcaccgg gttagagcaa ggcgggcagg
     5761 gcgtgcatat ccctttaccc gaaggggact ggcggaagca gaacctgaag ctgaatatgg
     5821 cactgaattt aagcggtacc ggcgatcttt ctgtggtgcc tgccgggcgt aatagcgaaa
     5881 tgaccttaac cagcaactgg ccgcatccca gttttttagg tgattttcta ccagccaaac
     5941 gggaagttag tgagtcaggt tttcaggcgc aatggcaaag cagctggttt gctaataatc
     6001 tcggtgagcg ttttgcttca ggcaatgata ccggctggga aaacttcccg gcgtttagcg
     6061 tcgcagtgac gacgccagcc gatcaatacc aattaactga ccgggcgact aagtatgcca
     6121 ttctgctgat tgcgctgact tttatggcgt tctttgtttt tgaaacgctc accgcgcaac
     6181 gcttacaccc aatgcaatat ttgctggtgg ggctttcatt ggtgatgttt tatttgctct
     6241 tgctggcgct ttctgaacat accggtttta ccgtggcatg gataatcgcc agtctgattg
     6301 gggcgttaat gaacggtatt tatttgcagg cggtattgaa aggttggcgc aacagcatgt
     6361 tgtttaccct cgcgctgttg ttgctggatg gtgtgatgtg gggactgctc aactctgccg
     6421 atagcgcgct gttgttggga accagtgtgc tggtggtggc gctggccggc atgatgtttg
     6481 tgacccgtaa tatcgactgg tatgcgtttt cactgccgaa aatgaaagcc agtaaagaag
     6541 ttacaacgga cgatgaatta cgtatctgga aataaggttg aaaaataaaa acggcgctaa
     6601 aaagcgccgt ttttttgacg gtggtaaagc cgattaatct tccagatcac cgcagaagcg
     6661 ataaccttca ccgtgaatgg tggcgatgat ttccggcgta tccggcgtag attcgaaatg
     6721 tttacgaata cggcggatcg tcacgtctac agtacggtcg tgcggtttca gctcacggcc
     6781 ggtcattttc ttcagcagtt cagcacggga ctgaattttg cctgggtttt cacagaagtg
     6841 aagcatggcg cggaactcac tgcgcggcag cttgtactgc tcgccatcag ggccgatcaa
     6901 cgaacggctg ttgatgtcca gttcccaacc attgaacttg tagctttcaa cgctacgacg
     6961 ttcttcgctg acagtaccca gattcatggt acgggacagc aggttgcgtg cacgaatcgt
     7021 cagttcacgc gggttgaacg gtttggtgat gtagtcatct gcaccgattt cgaggccgag
     7081 aattttatcg acttcgttgt cacggccagt caggaacatc aacgcaacat tcgcctgctc
     7141 gcgcagttca cgcgctaaca gaagaccgtt cttacccggc agattgatat ccatgatcac
     7201 caggttgatg tcatattcag agaggatctg atgcatttcc gcgccatctg tcgcttcgaa
     7261 aacatcatag ccttccgctt cgaaaatact tttcaacgtg ttgcgtgtta ccaactcgtc
     7321 ttcaacgata agaatgtgcg gggtctgcat gtttgctacc taaattgcca actaaatcga
     7381 aacaggaagt acaaaagtcc ctgacctgcc tgatgcatgc tgcaaattaa catgatcggc
     7441 gtaacatgac taaagtacgt aattgcgttc ttgatgcact ttccatcaac gtcaacaaca
     7501 tcattagctt ggtcgtgggt actttccctc aggacccgac agtgtcaaaa acggctgtca
     7561 tcctaaccat tttaacagca acataacagg ctaagagggg ccggacaccc aataaaacta
     7621 cgcttcgttg acatatatca agttcaattg tagcacgtta acagtttgat gaaatcatcg
     7681 tatctaaatg ctagctttcg tcacattatt ttaataatcc aactagttgc atcatacaac
     7741 taataaacgt ggtgaatcca attgtcgaga tttatttttt ataaaattat cctaagtaaa
     7801 cagaaggata tgtagcattt tttaacaatt caaccgttag tacagtcagg aaatagttta
     7861 gcctttttta agctaagtaa agggcttttt ctgcgactta cgttaagaat ttgtaaattc
     7921 gcaccgcgta ataagttgac agtgatcacc cggttcgcgg ttatttgatc aagaagagtg
     7981 gcaatatgcg tataacgatt attctggtcg cacccgccag agcagaaaat attggggcag
     8041 cggcgcgggc aatgaaaacg atggggttta gcgatctgcg gattgtcgat agtcaggcac
     8101 acctggagcc agccacccgc tgggtcgcac atggatctag tgatattatt gataatatta
     8161 aagttttccc gacactggct gaatcgttac acgatgtcga tttcactgtc gccaccactg
     8221 cgcgcagtcg ggcgaaatat cattactacg ccacgccagt tgaactggtg ccgctgttag
     8281 aggaaaaatc ttcatggatg agccatgccg cgctggtgtt tggtcgcgaa gattccgggt
     8341 tgactaacga agagttagcg ttggctgacg ttcttactgg tgtgccgatg gtggcggatt
     8401 atccttcgct caatctgggg caggcggtga tggtctattg ctatcaatta gcaacattaa
     8461 tacaacaacc ggcgaaaagt gatgcaacgg cagaccaaca tcaactgcaa gctttacgcg
     8521 aacgagccat gacattgctg acgactctgg cagtggcaga tgacataaaa ctggtcgact
     8581 ggttacaaca acgcctgggg cttttagagc aacgagacac ggcaatgttg caccgtttgc
     8641 tgcatgatat tgaaaaaaat atcaccaaat aaaaaacgcc tttgtaagta tttttcagct
     8701 tttcattctg actgcaacgg gcaatatgtc tctgtgtgga ttaaaaaaag agtgtctgat
     8761 agcagcttct gaactggtta catgccgtga gtaaattaaa attttattga cttaggtcac
     8821 taaatacttt aaccaatata ggcatagcgc acagacagat aaaaattaca gagtacacaa
     8881 catccatgaa acgcattagc accaccatta ccaccaccat caccattacc acaggtaacg
     8941 gtgcgggctg acgcgtacag gaaacacaga aaaaagcccg cacctgacag tgcgggcttt
     9001 ttttttcgac caaaggtaac gaggtaacaa ccatgcgagt gttgaagttc ggcggtacat
     9061 cagtggcaaa tgcagaacgt tttctgcggg ttgccgatat tctggaaagc aatgccaggc
     9121 aggggcaggt ggccaccgtc ctctctgccc ccgccaaaat caccaaccac ctggtggcga
     9181 tgattgaaaa aaccattagc ggccaggatg ctttacccaa tatcagcgat gccgaacgta
     9241 tttttgccga acttttgacg ggactcgccg ccgcccagcc ggggttcccg ctggcgcaat
     9301 tgaaaacttt cgtcgatcag gaatttgccc aaataaaaca tgtcctgcat ggcattagtt
     9361 tgttggggca gtgcccggat agcatcaacg ctgcgctgat ttgccgtggc gagaaaatgt
     9421 cgatcgccat tatggccggc gtattagaag cgcgcggtca caacgttacc gttatcgatc
     9481 cggtcgaaaa actgctggca gtggggcatt acctcgaatc taccgtcgat attgctgagt
     9541 ccacccgccg tattgcggca agtcgtattc cggctgatca catggtgctg atggcaggtt
     9601 tcaccgccgg taatgaaaaa ggcgaactgg tggtgcttgg acgcaacggt tccgactact
     9661 ctgctgcggt gctggctgcc tgtctacgcg ccgattgttg cgagatttgg acggacgttg
     9721 acggggtcta tacctgcgac ccgcgtcagg tgcccgatgc gaggttgttg aagtcgatgt
     9781 cctaccagga agcgatggag ctttcctact tcggcgctaa agttcttcac ccccgcacaa
     9841 ttacccctat cgcccagttc cagatccctt gcctgattaa aaataccgga aatcctcaag
     9901 caccaggtac gctcattggt gccagccgtg atgaagacga attaccggtc aagggcattt
     9961 ccaatctgaa taacatggca atgttcagcg tttccggccc ggggatgaaa gggatggttg
    10021 gcatggcggc gcgtgtcttt gcagcgatgt cacgcgcccg tatttccgtg gtgctgatta
    10081 cgcaatcgtc ttctgaatac agtatcagtt tctgcgttcc acaaagcgac tgtgtgcgag
    10141 ctgaacgggc gatgcaggaa gagttctatc ttgaactgaa ggaaggcttg ctggagccgc
    10201 tggcggtgac ggaacggctg gccattatct cggtggtagg tgatggtatg cgcaccttgc
    10261 gtgggatctc ggcgaaattc tttgccgcgc tggcccgcgc caatatcaac attgtcgcca
    10321 ttgctcaggg atcttctgaa cgctcaatct ctgtcgtggt caataacgat gatgcgacca
    10381 ctggcgtgcg cgttactcat cagatgctgt tcaacaccga tcaggttatc gaagtgtttg
    10441 tgattggtgt cggtggcgtt ggcggtgcgc tgctggagca actgaagcgt cagcaaagct
    10501 ggttgaagaa taaacatatc gacttacgtg tctgcggtgt tgccaactcg aaggcactgc
    10561 tcaccaatgt acatggcctt aatctggaaa actggcagga agaactggcg caagccaaag
    10621 agccgtttaa tctcgggcgc ttaattcgcc tcgtgaaaga atatcatctg ctgaacccgg
    10681 tcattgttga ctgcacttcc agccaggcag tggcggatca atatgccgac ttcctgcgcg
    10741 aaggtttcca cgttgtcacg ccgaacaaaa aggccaacac ctcgtcgatg gattactacc
    10801 atcagttgcg ttatgcggcg gaaaaatcgc ggcgtaaatt cctctatgac accaacgttg
    10861 gggctggatt accggttatt gagaacctgc aaaatctgct caatgcaggt gatgaattga
    10921 tgaagttctc cggcattctt tctggttcgc tttcttatat cttcggcaag ttagacgaag
    10981 gcatgagttt ctccgaggcg accacgctgg cgcgggaaat gggttatacc gaaccggacc
    11041 cgcgagatga tctttctggt atggatgtgg cgcgtaagct attgattctc gcccgtgaaa
    11101 cgggacgtga actggaactg gcggatattg aaattgaacc tgtgctgccc gcagagttta
    11161 acgcagaggg tgatgttgcc gcttttatgg cgaatctgtc acagctcgac gatctctttg
    11221 ccgcacgcgt ggcgaaggct cgtgatgagg gcaaagtttt gcgctatgtt ggcaatattg
    11281 atgaagatgg catctgccgc gtgaagattg ccgaagtgga tggcaatgat ccgctgttca
    11341 aagtgaaaaa tggcgaaaac gccctggcct tctatagcca ctattatcag ccgctgccgt
    11401 tggttctgcg cggatatggc gcgggcaatg acgttacagc tgctggtgtc tttgccgatc
    11461 tgctacgtac cctctcatgg aagttaggag tctgacatgg ttaaagttta tgccccggct
    11521 tccagtgcca atatgagcgt cgggtttgat gtgctcgggg cggcggtgac acctgttgat
    11581 ggtgcattgc tcggagatgt agtcacggtt gaggcggcag agacattcag tctcaacaac
    11641 ctcggacgct ttgccgataa gctgccgtca gaaccacggg aaaatatcgt ttatcagtgc
    11701 tgggagcgtt tttgccagga actgggtaag caaattccag tggcgatgac cctggaaaag
    11761 aatatgccga tcggttcggg cttaggctcc agtgcctgtt cggtggtcgc ggcgctgatg
    11821 gcgatgaatg aacactgcgg caagccgctt aatgacactc gtttgctggc tttgatgggc
    11881 gagctggaag gccgtatctc cggcagcatt cattacgaca acgtggcacc gtgttttctt
    11941 ggtggtatgc agttgatgat cgaagaaaac gacatcatca gtcagcaagt gccagggttt
    12001 gatgagtggc tgtgggtgct ggcgtatccg gggattaaag tctcgacggc agaagcccgg
    12061 gctattttac cggcgcagta tcgccgccag gattgcattg cgcacgggcg acatctggca
    12121 ggcttcattc acgcctgcta ttcccgtcag cctgagcttg ccgcgaagct gatgaaagat
    12181 gttatcgctg aaccctaccg tgaacggtta ctgccaggct tccggcaggc gcggcaggcg
    12241 gtcgcggaaa tcggcgcggt agcgagcggt atctccggct ccggcccgac cttgttcgct
    12301 ctgtgtgaca agccggaaac cgcccagcgc gttgccgact ggttgggtaa gaactacctg
    12361 caaaatcagg aaggttttgt tcatatttgc cagctggata cggcgggcgc acgagtactg
    12421 gaaaactaaa tgaaactcta caatctgaaa gatcacaatg agcaggtcag ctttgcgcaa
    12481 gccgtaaccc aggggttggg caaaaatcag gggctgtttt tcccgcacga cctgccggaa
    12541 ttcagcctga ctgaaattga tgagatgctg aagctggatt ttgtcacccg cagtgcgaag
    12601 atcctctcgg cgtttattgg tgatgaaatc ccgcaggaaa tcctggaaga gcgcgtacgc
    12661 gcggcgtttg ccttcccggc tccggtcgcc aatgttgaaa gcgatgtcgg ttgtctggaa
    12721 ttgtttcacg ggccaacgct ggcatttaaa gatttcggcg gtcgctttat ggcacaaatg
    12781 ctgacccata ttgcgggcga taagccagtg accattctga ccgcgacatc tggtgatact
    12841 ggagcggcag tggctcatgc tttctacggt ttaccgaatg tgaaagtggt tatcctctat
    12901 ccacgaggca aaatcagtcc actgcaagaa aaactgttct gtacattggg cggcaatatc
    12961 gaaactgttg ccatcgacgg cgatttcgat gcctgtcagg cgctggtgaa gcaggcgttt
    13021 gatgatgaag aactgaaagt ggcgctgggg ctaaactctg ctaactccat taacatcagc
    13081 cgtttgctgg cgcagatttg ctactacttt gaagctgttg cgcagctgcc gcaggaagcg
    13141 cgcaaccagc tggttgtctc ggtgccaagc ggaaacttcg gcgatttgac ggcgggtctg
    13201 ctggcgaagt cactcggtct gccggtgaaa cgttttattg ctgcgaccaa cgtgaacgat
    13261 accgtgccac gtttcctgca cgacggtcag tggtcaccca aagcgactca ggcgacgtta
    13321 tccaatgcga tggatgttag ccagccaaac aactggccgc gtgtggaaga gttgttccgc
    13381 cgcaaaatct ggcaactgaa agagctgggg tatgcagccg tggatgatga aaccacgcaa
    13441 cagacaatgc gtgagttaaa agaactgggc tatacctcgg agccgcacgc tgccgtagct
    13501 tatcgtgcgc tgcgtgacca gttgcatcca ggcgaatatg gcttgttcct cggcaccgcg
    13561 catccggcga aatttaaaga gagcgtggaa gcgattctcg gtgaaacatt ggatctgcca
    13621 aaagagctgg cagaacgtgc tgatttaccc ttgctttcgc ataacctgcc cgccgatttt
    13681 gctgcgttgc gtaaattgat gatgaatcat cagtaacatc tattcattat ctcaatcagg
    13741 ccgggtttgc ttttatgcag cccggctttt ttatgaagaa aatatggaga aaaacgacag
    13801 ggaaaaagga gaaattctca ataaatgcgg taacttagag attaggattg cggagaataa
    13861 caactgccgt tctcatcgcg taatctccgg atatcgaccc ataacgggca atgataaaag
    13921 gagtaacctg tgaaaaagat gcaatctatc gtactcgcac tttccctggt tctggtcgct
    13981 cccatggcag cacaggctgc ggaaattacg ttagtcccgt cagtaaaatt acagataggc
    14041 gatcgtgata atcgtggcta ttactgggat ggcggtcact ggcgcgacca cggctggtgg
    14101 aaacaacatt atgaatggcg aggcaatcgc tggcacccac acggaccgcc gccatcgccg
    14161 cgccataaca agcacaatga tcatcgtggc gatcatcgtc cggggcctga caaacatcat
    14221 cgctaaatat caatgccgga ttgccatccg gcatttactg caaacttaac gctgctcgta
    14281 gcgtttaaac accagttcgc cattgctgga ggaagcttca tcaaagaagt aaccttcgct
    14341 attaaaacca gtcagttgct ctggtttggt cagccgattt tcaataatga aacgactcat
    14401 cagaccgcgt gctttcttag cgtagaagct gattatctta aatttgccgt tcttctcatc
    14461 gaggaacact ggcttgataa tctcggcatt caatttcttc ggcttcaccg atttaaaata
    14521 ctcatctgac gccagattta tcaccacatt atcgccttgt gctgcgagcg cctcgttcag
    14581 cttgttggtg atgatatctc cccagaattg atacagatct ttccctcggg cattctcaag
    14641 acgtatcccc atttccagac gataaggctg cattaaatcg agcgggcgca gtacgccata
    14701 caagccggaa agcattcgca aatgctgttg ggcaaaatcg aaatcgtctt cgctgaaggt
    14761 ttcggcctgc aagccggtgt agacatcacc tttaaatgcc agaatcgcct ggcgggcatt
    14821 ctccgacgtg aaatctggct gccagtcatg aaagcgagcg gcgttgatac ccgccagttt
    14881 gtcgctgatg cgcatcaggg agctaatctg cgggggcgtc agtttccgcg cttcatggat
    14941 cagctgctgg gagttgtcta acagctccgg cagcgtatag cgcgtggtgg gcaacgggct
    15001 ttggtaatca agcgttttcg caggtgaaat aagaatcagc atatccagtc cttgcaggaa
    15061 atttatgccg actttagcaa aaaaagagaa tgagttgatc gatagttgtg attactcctg
    15121 cgaaacgtca tcccacgcgt ctggagaaag ctggcgaccg atctccggat aacgcaacgg
    15181 atcaaacacc gggcgcacgc cgagtttacg ctggcgtaga taatcactgg caatggtatg
    15241 aaccacaggc gagagcagta atatggcggt caaattggta atagccatgc aggccattat
    15301 gatatctgcc agttgccaca tcagcggaag acttagcaag gtgccgccga tgaccgttgc
    15361 gaaggtgcag atccgcagac accagatcgc tttagggttg ttcaggcgta aaaagaagag
    15421 attgttttcg gcataaatgt agttggcaac gatggagctg aaggcaaaca gaataaccac
    15481 gagggtaaca aactcagcac cccaggaacc cattaacacc cgcatcgcct tctggataag
    15541 ctgaatacct tccagcggca tgtaggttgt gccgttaccc gccagtaata tcagcatggc
    15601 gcttgccgta cagatgacca gggtgtcgat aaaaatgcca atcatctgga caattccttg
    15661 cgctgcggga tgcggaggcc aggacgccgc tgccgctgcc gcgtttggcg tcgaccccat
    15721 tcccgcctca ttggaaaaca tactgcgctg aaaaccgtta gtaatcgcct ggcttaaggt
    15781 atatcccgcc gcgccgcctg ccgcttcctg ccagccaaaa gcactctcaa aaatagacca
    15841 aatgacgtgg ggaagttgcc cgatattcat tacgcaaatt accaggctgg tcagtaccca
    15901 gattatcgcc atcaacggga caaacccctg catgagccgg gcgacgccat gaagaccgcg
    15961 agtgattgcc agcagagcaa agacagcgag aataatgcct gtcaccagcg gaggaaaatc
    16021 aaaagaaaaa ctcagggctc gggcaacggc gttcgcctga actccgctga aaattatgcc
    16081 gtaggcgatg agcaaaaaga gggcgaacag aacgcccatc cagcgcatcc ccagcccgcg
    16141 cgccatatac catgccggtc cgccacgaaa ctgcccatta acgtcacgtt ctttataaag
    16201 ttgtgcaagg gaacattcgg caaacgaggt cgccatgccg ataaacgcgg caacccacat
    16261 ccagaagacg gcacctggtc caccggcggt aatagccagc gcaacgccag ccaggttgcc
    16321 gctacccacg cgcgccgcaa gactggtaca caatgactga aatgaggtta aaccgcctgg
    16381 ctgtggatga atgctatttt taagactttt gccaaactgg cggatgtagc gaaactgcac
    16441 aaatccggtg cgaaaagtga accaacaacc tgcgccgaag agcaggtaaa tcattaccga
    16501 tccccaaagg acgctgttaa taaaggagaa aaaatctggc atgcatatcc ctcttattgc
    16561 cggtcgcgat gactttcctg tgtaaacgtt accaattgtt taagaagtat atacgctacg
    16621 aggtacttga taacttctgc gtagcataca tgaggttttg tataaaaatg gcgggcgata
    16681 tcaacgcagt gtcagaaatc cgaaacagtc tcgcctggcg ataaccgtct tgtcggcggt
    16741 tgcgctgacg ttgcgtcgtg atatcatcag ggcagaccgg ttacatcccc ctaacaagct
    16801 gtttaaagag aaatactatc atgacggaca aattgacctc ccttcgtcag tacaccaccg
    16861 tagtggccga cactggggac atcgcggcaa tgaagctgta tcaaccgcag gatgccacaa
    16921 ccaacccttc tctcattctt aacgcagcgc agattccgga ataccgtaag ttgattgatg
    16981 atgctgtcgc ctgggcgaaa cagcagagca acgatcgcgc gcagcagatc gtggacgcga
    17041 ccgacaaact ggcagtaaat attggtctgg aaatcctgaa actggttccg ggccgtatct
    17101 caactgaagt tgatgcgcgt ctttcctatg acaccgaagc gtcaattgcg aaagcaaaac
    17161 gcctgatcaa actctacaac gatgcaggga ttagcaacga tcgtattctg atcaaactgg
    17221 cttctacctg gcagggtatc cgtgctgcgg aacagctgga aaaagaaggc atcaactgta
    17281 acctgaccct gctgttctcc tttgctcagg ctcgtgcttg tgcggaagcg ggcgtgttcc
    17341 tgatctcgcc gtttgttggc cgtattcttg actggtacaa agcgaatacc gataagaaag
    17401 agtacgctcc ggcagaagat ccgggcgtgg tttctgtatc tgaaatctac cagtactaca
    17461 aagagcacgg ttatgaaacc gtggttatgg gcgcaagctt ccgtaacatc ggcgaaattc
    17521 tggaactggc aggctgcgac cgtctgacca tcgcaccggc actgctgaaa gagctggcgg
    17581 agagcgaagg ggctatcgaa cgtaaactgt cttacaccgg cgaagtgaaa gcgcgtccgg
    17641 cgcgtatcac tgagtccgag ttcctgtggc agcacaacca ggatccaatg gcagtagata
    17701 aactggcgga aggtatccgt aagtttgctg ttgaccagga aaaactggaa aaaatgatcg
    17761 gcgatctgct gtaatcattc ttagcgtgac cgggaagtcg gtcacgctac ctcttctgaa
    17821 gcctgtctgt cactcccttc gcagtgtatc attctgttta acgagactgt ttaaacggaa
    17881 aaatcttgat gaatacttta cgtattggct tagtttccat ctctgatcgc gcatccagcg
    17941 gcgtttatca ggataaaggc atccctgcgc tggaagaatg gctgacatcg gcgctaacca
    18001 cgccgtttga actggaaacc cgcttaatcc ccgatgagca ggcgatcatc gagcaaacgt
    18061 tgtgtgagct ggtggatgaa atgagttgcc atctggtgct caccacgggc ggaactggcc
    18121 cggcgcgtcg tgacgtaacg cccgatgcga cgctggcagt agcggaccgc gagatgccag
    18181 gctttggtga acagatgcgc cagatcagcc tgcattttgt accaactgcg atcctttctc
    18241 gtcaggtggg tgtgattcgc aaacaggcgc tgatccttaa cttacccggt cagccgaagt
    18301 cgattaaaga gacgctggaa ggtgtaaagg acgctgaggg taacgttgtg gtgcacggta
    18361 tttttgccag cgtaccgtac tgcattcagt tgctggaagg gccatacgtt gaaacggcac
    18421 cggaagtggt tgctgcattc agaccgaaga gtgcaagacg cgaagttagc gaataaaaaa
    18481 atccccccgg gcggggggat ctcaaaacaa ttagtgcgat tcaccaatcg gcagaacggt
    18541 gcgaccaaac tgctcgttca gcacttcacc catcgccaga tagattgcgc tggcaccgca
    18601 gatcagacca atccagccgg caaagtggat gatcgcggca ttaccgacaa tgttaccgat
    18661 cgccagcagg gcaaacagca cggtcaggct aaagaaaacg aattgcagaa cgcgtgcgcc
    18721 tttcagcgtg ccgaagaaca taaacagcgt aaatacgccc cacagaccca ggtagacacc
    18781 aaggaactgt gcatttggcg catcggtcag acccagtttc ggcatcagca gaatcgcaac
    18841 cagcgtcagc cagaaagaac cgtaagaggt gaatgcggtt aaaccgaaag tgttgccttt
    18901 tttgtactcc agcagaccag caaaaatttg cgcgatgccg ccgtagaaaa tgcccattgc
    18961 aagaataata ccgtcaagag cgaaataacc cacgttgtgc aggttaagca gaatggtggt
    19021 catgccgaag cccatcaggc ccagcggtgc cggattagcc aacttagtgt tgcccataat
    19081 tcctcaaaaa tcatcatcga atgaatggtg aaataatttc cctgaataac tgtagtgttt
    19141 tcagggcgcg gcataataat cagccagtgg ggcagtgtct acgatctttt gaagggaaaa
    19201 tgaaaatttt ccccggtttc cggtatcaga cctgagtggc actaaccatc cggcgcaggc
    19261 aggcgatttg cagtacggct ggaatcgtca cgcgataggc gctgccgctg accgctttaa
    19321 ccccatttag tgccgcgcct acagggcctc ccagccccgc gccgcgcagc aaaccatgcc
    19381 caagtacgct cattgctgcg tgggtgcgta aaatgcgggt caattggctg gaaagcaaat
    19441 gcgacacgcc ttttgccaat aatttgtctt tcatcagcag cggcagcagc tcttccagct
    19501 cattcaccct ggcatcgacc gcgtgcagaa actcctgctt atgttcctcg tccattttct
    19561 tccaggtatt acgcagaaat tgttccagta actgttgctc aatttcaaac gtagacatct
    19621 ctttgtcggc tttcagcttc aatcgttttg aaacatcgag caaaatggcc cgatacaatt
    19681 taccgtgtcc gcgcagtttg ttggcgatac tatcgccacc aaaatgttgt aattctccgg
    19741 caatcagctg ccagttgcgg cggtgttgct cgggatgtcc ttccatcgat ttaaacagtt
    19801 cgttgcgcat cagtacgctg gagaggcgag ttttgccttt ttcattatgg gtgagcagcc
    19861 gggcgaaatt tgccaattgt tcctcactac aatgctggag aaaatccaga tctgaatcat
    19921 tcaggtaatt aacattcatt ttttttgctt ctatattctg gcgttagtcg tcgccgataa
    19981 ttttcagcgt ggccatatcc gatgagttca ccgtatggcc ggaaaaggtg atttttgaga
    20041 cgcagcgttt attgtcgtta tcgctgttaa tgttgatcca gtcagtggtt tgcccttctt
    20101 ttatttctga aggaatattc aggctctgac tggcgctacg ggcggctttg aaataaaccg
    20161 atgcaccact taactgtaaa tcgccatggt cggcagtgag ttgaatgcgt ttaacaatgc
    20221 gacaaacagg aagtttcagc gccagatcgt tggtttcgtt tcgcggcatt gcaatgacgc
    20281 cgaggatttt atggtcgttt gcctgcgccg tgcagcacag cagcaggcta atcgccaggc
    20341 tggcggaaag cgtaaaaacg gatttcataa ggattctctt agtagggaga ggtaggggat
    20401 gaatacccac tagtttactg ctgataaaga gaagattcag gtacgtaata ttttcttttt
    20461 attacaattt ttctatgaat gtcttggctg tcattcattc tctatatgaa taaaattgct
    20521 gtcagtttca tgtcttgtcc tgccatatcg cgaaatttct gcgcaaaagc acaaaaaatt
    20581 tttgcatctc ccccttgatg acgtgggtta cgaccccatt tagtagtcaa ccgcagtgag
    20641 tgagtctgca aaaaaatgaa attgggcagt tgaaaccaga cgtttcgccc ctattacaga
    20701 ctcacaacca catgatgacc gaatatatag tggagacgtt tagatgggta aaataattgg
    20761 tatcgacctg ggtactacca actcttgtgt agcgattatg gatggcacca ctcctcgtgt
    20821 actggagaac gccgaaggcg atcgcaccac gccttctatc attgcctata cccaggatgg
    20881 tgaaactctg gttggtcagc cggctaaacg tcaggcagtg acgaacccgc aaaacaccct
    20941 gtttgcgatt aaacgcctga ttggccgccg cttccaggac gaagaagtac agcgtgatgt
    21001 ttccatcatg ccgttcaaaa ttattgctgc tgataacggc gacgcatggg tcgaagttaa
    21061 aggccagaaa atggcaccgc cgcagatctc tgctgaagtg ctgaaaaaaa tgaagaaaac
    21121 cgctgaagat tacctgggtg aaccggtaac tgaagctgtt atcaccgtac cggcatactt
    21181 taacgatgct cagcgtcagg caaccaaaga cgcaggccgt atcgctggtc tggaagtaaa
    21241 acgtatcatc aacgaaccga ccgcagctgc gctggcttac ggtctggaca aaggtactgg
    21301 caaccgtact atcgcggttt atgacctggg tggtggtact ttcgatattt ctattatcga
    21361 aatcgacgaa gttgacggcg aaaaaacctt cgaagttctg gcaaccaacg gtgataccca
    21421 cctggggggt gaagacttcg acagccgtct gatcaactac ctggttgaag aattcaagaa
    21481 agatcagggc attgacctgc gcaacgatcc gctggcaatg cagcgcctga aagaagcggc
    21541 agaaaaagcg aaaatcgaac tgtcttccgc tcagcagacc gacgttaacc tgccgtacat
    21601 cactgcagac gcgaccggtc cgaaacacat gaacatcaaa gtgactcgtg cgaaactgga
    21661 aagcctggtt gaagatctgg taaaccgttc cattgagccg ctgaaagttg cactgcagga
    21721 cgctggcctg tccgtatctg atatcgacga cgttatcctc gttggtggtc agactcgtat
    21781 gccaatggtt cagaagaaag ttgctgaatt ctttggtaaa gagccgcgta aagacgttaa
    21841 cccggacgaa gctgtagcaa tcggtgctgc tgttcagggt ggtgttctga ctggtgacgt
    21901 gaaagacgta ctgctgctgg acgttacccc gctgtctctg ggtatcgaaa ccatgggcgg
    21961 tgtgatgacg acgctgatcg cgaaaaacac cactatcccg accaagcaca gccaggtgtt
    22021 ctctaccgct gaagacaacc agtctgcggt aaccatccac gtgctgcagg gtgaacgtaa
    22081 acgtgcggct gataacaaat ctctgggtca gttcaacctg gatggtatca acccggcacc
    22141 gcgcggcatg ccgcagatcg aagttacctt cgatatcgat gctgacggta tcctgcacgt
    22201 ttccgcgaaa gataaaaaca gcggtaaaga gcagaagatc accatcaagg cttcttctgg
    22261 tctgaacgaa gatgaaatcc agaaaatggt acgcgacgca gaagctaacg ccgaagctga
    22321 ccgtaagttt gaagagctgg tacagactcg caaccagggc gaccatctgc tgcacagcac
    22381 ccgtaagcag gttgaagaag caggcgacaa actgccggct gacgacaaaa ctgctatcga
    22441 gtctgcactg actgcactgg aaactgctct gaaaggtgaa gacaaagccg ctatcgaagc
    22501 gaaaatgcag gaactggcac aggtttccca gaaactgatg gaaatcgccc agcagcaaca
    22561 tgcccagcag cagactgccg gtgctgatgc ttctgcaaac aacgcgaaag atgacgatgt
    22621 tgtcgacgct gaatttgaag aagtcaaaga caaaaaataa tcgccctata aacgggtaat
    22681 tatactgaca cgggcgaagg ggaatttcct ctccgcccgt gcattcatct aggggcaatt
    22741 taaaaaagat ggctaagcaa gattattacg agattttagg cgtttccaaa acagcggaag
    22801 agcgtgaaat caaaaaggcc tacaaacgcc tggccatgaa ataccacccg gaccgtaacc
    22861 agggtgacaa agaggccgag gcgaaattta aagagatcaa ggaagcttat gaagttctga
    22921 ccgactcgca aaaacgtgcg gcatacgatc agtatggtca tgctgcgttt gagcaaggcg
    22981 gcatgggcgg cggcggtttt ggcggcggcg cagacttcag cgatattttt ggtgacgttt
    23041 tcggcgatat ttttggcggc ggacgtggtc gtcaacgtgc ggcgcgcggt gctgatttac
    23101 gctataacat ggagctcacc ctcgaagaag ctgtacgtgg cgtgaccaaa gagatccgca
    23161 ttccgactct ggaagagtgt gacgtttgcc acggtagcgg tgcaaaacca ggtacacagc
    23221 cgcagacctg tccgacctgt catggttctg gtcaggtgca gatgcgccag ggcttcttcg
    23281 ctgtacagca gacctgtcca cactgtcagg gccgcggtac gctgatcaaa gatccgtgca
    23341 acaaatgtca tggtcatggt cgtgttgagc gcagcaaaac gctgtccgtt aaaatcccgg
    23401 caggggtgga cactggagac cgcatccgtc ttgcgggcga aggtgaagcg ggcgagcatg
    23461 gcgcaccggc aggcgatctg tacgttcagg ttcaggttaa acagcacccg attttcgagc
    23521 gtgaaggtaa caacctgtat tgcgaagtcc cgatcaactt cgcgatggca gcgctgggtg
    23581 gcgaaatcga agtaccgacc cttgatggtc gcgtcaaact gaaagtgcct ggcgaaaccc
    23641 agaccggtaa gctgttccgt atgcgtggta aaggcgtcaa gtctgttcgc ggcggtgcgc
    23701 aaggggactt gctgtgccgc gttgtcgtcg aaacaccggt aggtctgaac gagaagcaga
    23761 aacagctgct gcaagagctg caggaaagct tcggtggccc aaccggcgag cacaacagcc
    23821 cgcgttcaaa gagcttcttt gatggtgtga agaagttttt tgacgacctg actcgctaac
    23881 ctccccaaaa gcctgcccgt gggcaggcca gggtaaaaat agggtgcgtt gaagatatgc
    23941 gagcacctgt aaagtggcgg ggatcactcc ccgccgttgc tcttactcgg attcgtaagc
    24001 cgtgaaaaca gcaacctccg tctggccagt tcggatgtga acctcacaga ggtcttttct
    24061 cgttaccagc gccgccacta cggcggtgat acagatgacg atcagggcga caatcatcgc
    24121 cttatgctgc ttcattgctc tcttctcctt gaccttacgg tcagtaagag gcactctaca
    24181 tgtgttctgc atataggggg cctcgggttg atggtaaaat atcactcggg gcttttctct
    24241 atctgccgtt cagctaatgc ctgagacaga cagcctcaag cacccgccgc tattatatcg
    24301 ctctctttaa cccattctgt tttatcgatt ctaatcctga agacgcctcg catttttgcg
    24361 taatcaatct ttagtacatt gtttatttta actcttatgt gcgttttcat ttacaaaagt
    24421 tgagattatg aacaagaggt ttaagttatg cctgaatggt gagttattac tggagaaagt
    24481 aatagaagat acaaaatatt cttgtacaga attgaatttt ctttttgtga ggaaaaatgt
    24541 ttataaatca atgagtgata tttaaaatcg acacactttt tgaaacacga tacctccaca
    24601 cccgggggta aaagtaatct gcgatctcca tcacgctttg ccattcacct ttaaatatac
    24661 tttctgagtc gttcttaatc aaaatgaatt atctgatgta ttgatttcta tggaaccata
    24721 aaaaaaatga atggagagca tattatatgt ctgccaaaag acggcttctt attgcgtgtg
    24781 ccctgacaac gattttctat caccttcctg catattcttc attagaatat aaaggttcct
    24841 ttggctcaat aaatgcaggt tatgctgact ggaatagtgg cttcgttaac actcatcgtg
    24901 gtgaagtatg gaaagtgact gcagactttg gtgtgaattt taaagaagcc gagttttact
    24961 cattttatga aagcaatgtg cttaaccatg ccgtagcggg gcgtaatcat accgtttctg
    25021 ctatgacaca tgtcagactc tttgactcca atatgacttt ctttggcaag atctatggtc
    25081 aatgggataa ctcatggggt gacgatcttg atatgtttta tggtttgggc tacctcggct
    25141 ggaatggttc atgggggttt tttaaaccgt atattggatt acataatcaa tctggagact
    25201 acgtctcagc caaatatgga caaacaaatg gctggaatgg ttatgttgtt ggctggacag
    25261 cagtattacc ttttacgtta tttgacgaaa aatttgtttt atctaactgg aatgaaatag
    25321 aactggacag aaatgatgcc tatgcggaac aacaatttgg tcggaatggg ctaaatggcg
    25381 gtttaacagt ggcctggaag ttctatcctc gctggaaagc cagtgtgacg tggcgttatt
    25441 tcgataataa gttgggctac gatggttttg gcgatcaaat gatttatatg cttggttatg
    25501 atttctaatt ttcatcaggg aataattatg cagaaaacat taatggccag tttgatcggc
    25561 cttgcagttt gcacagggaa tgcttttaat ccagccgtag ccgccgaaac taaacaaccc
    25621 aatttagtca tcattatggc ggatgattta ggctatggtg atttggcaac atatggacat
    25681 cagatcgtta aaacgcccaa tatagacagg cttgcccagg aaggcgtcaa atttaccgac
    25741 tattatgcac ctgccccctt aagttctcct tcacgggcgg gactattaac agggcggatg
    25801 ccatttcgta ccggcatacg ctcatggatc ccaacgggaa aagatgtggc attagggcgt
    25861 aatgaactca cgattgctaa tctactcaaa gcgcaagggt acgacacggc catgatgggt
    25921 aagctgcatc tgaatgcagg cggcgatcgc accgatcagc cgcaggcaaa agatatgggc
    25981 tttgattact cac
//
LOCUS       DAMZFT010000067        25525 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.067, whole genome shotgun sequence.
ACCESSION   DAMZFT010000067 DAMZFT010000000
VERSION     DAMZFT010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 25525)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25525)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25525
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.067"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(<1..919)
                     /gene="ydcO"
                     /locus_tag="PN977_002830"
     CDS             complement(<1..919)
                     /gene="ydcO"
                     /locus_tag="PN977_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001748359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BenE family transporter YdcO"
                     /protein_id="HDI6042848.1"
                     /translation="MLRCLYAITHEVTMRLFSIPPPTLLAGFLAVLIGYASSAAIIWQ
                     AAIVAGATTAQISGWMTALGLAMGVSTLALTLWYRVPVLTAWSTPGAALLVTGLQGLT
                     LNETIGVFIVTNALIVLCGITGLFAHLMRIIPHSLAAAMLAGILLRFGLQAFASLDGQ
                     FTLCGSMLLVWLATRAVAPRYAVIAAMIIGVVIVIAQGDIVTTDVVFKPVLPTYISPD
                     FSFAHSLSVALPLFLVTMASQNAPGIAAMKAAGYSAPVSPLIVFTGLLALVFSPFGVY
                     SVGIAAITAAICQSPEAHPDKDQRWLAAAV"
     gene            972..1508
                     /gene="sutR"
                     /locus_tag="PN977_002831"
     CDS             972..1508
                     /gene="sutR"
                     /locus_tag="PN977_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SutR"
                     /protein_id="HDI6042849.1"
                     /translation="MENLARFLSTTLKQLRQQRGWSLSRLAEATGVSKAMLGQIERNE
                     SSPTVATLWKIATGLNVPFSTFISPPQSATPSVYDPQQQAMVITSLFPYDPQLCFEHF
                     SIQMASGAISESTPHEKGVIEHVVVIDGQLDLCVDGEWQSLNCGEGVRFAADVTHIYR
                     NGGEQTVHFHSLIHYPRS"
     gene            1581..3542
                     /gene="rlhA"
                     /locus_tag="PN977_002832"
     CDS             1581..3542
                     /gene="rlhA"
                     /locus_tag="PN977_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA 5-hydroxycytidine C2501 synthase"
                     /protein_id="HDI6042850.1"
                     /translation="MTVSSHRLELLSPARDAAIAREAILHGADAVYIGGPGFGARHNA
                     SNSLKDIAELVPFAHRYGAKIFVTLNTILHDDELEPAQRLITDLYQTGVDALIVQDMG
                     ILELDIPPIELHASTQCDIRTVEKAKFLSDVGFTQIVLARELNLEQIRAIHQATDATI
                     EFFIHGALCVAYSGQCYISHAQTGRSANRGDCSQACRLPYTLKDDQGRVVSYEKHLLS
                     MKDNDQTANLGALIDAGVRSFKIEGRYKDMSYVKNITAHYRQMLDAIIEERGDLARAS
                     SGRTEHFFVPSTEKTFHRGSTDYFVNARKGDIGAFDSPKFIGLPVGEVLKVAKDHLDV
                     AVSEPLANGDGLNVMIKREVVGFRANTVEKTGENQYRVWPNEMPAELHKIRPHHPLNR
                     NLDHDWQQALTKTSSERRVAVDIELGGWQEQLILTLTSEEGVSITHTLDGQFDEANNA
                     EKAMNNLKDGLAKLGQTIYYARDVQINLPGALFVPNSLLNQFRREAADMLDAARLASY
                     QRGSRKPVADPAPVYPQTHLSFLANVYNQKAREFYHRYGVQLIDAAYEAHEEKGEVPV
                     MITKHCLRFAFNLCPKQAKGNIKSWKATPMQLVNGDEVLTLKFDCRPCEMHVIGKIKN
                     HILKMPLPGSVVASVSPDDLLKTLPKRKG"
     gene            complement(3634..3864)
                     /locus_tag="PN977_002833"
     CDS             complement(3634..3864)
                     /locus_tag="PN977_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2554 family protein"
                     /protein_id="HDI6042851.1"
                     /translation="MFSKALSVVLLTCALFSGQLMAGHKGHEFMWVKNVDHQLRHEAD
                     SDELRAVAEESAEGLREHFYWQKSRKPEAGQR"
     gene            4086..4262
                     /gene="hicA"
                     /locus_tag="PN977_002834"
     CDS             4086..4262
                     /gene="hicA"
                     /locus_tag="PN977_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin HicA"
                     /protein_id="HDI6042852.1"
                     /translation="MKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIK
                     EPLRKAILKQLGLS"
     gene            4308..4724
                     /gene="hicB"
                     /locus_tag="PN977_002835"
     CDS             4308..4724
                     /gene="hicB"
                     /locus_tag="PN977_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HicB"
                     /protein_id="HDI6042853.1"
                     /translation="MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALL
                     TAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKVLLLNAFLQSEITQQELARRIGK
                     PKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV"
     gene            4803..6209
                     /locus_tag="PN977_002836"
     CDS             4803..6209
                     /locus_tag="PN977_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HDI6042854.1"
                     /translation="MKKYQQLAEQLREQIASGIWQPGDRLPSLRDQVALSGMSFMTVS
                     HAYQLLESQGYIIARPQSGYYVAPQAIKMPKAPVIPVTRDEAVDINTYIFDMLQASRD
                     PSVVPFASAFPDPRLFPLQQLNRSLAQVSKTATAMSVIENLPPGNAELRQAIARRYAL
                     QGITISPDEIAITAGALEALNLSLQVVTEPGDWVIVENPCFYGALQALERLRLKALSV
                     ATDVKEGIDLQALELALQEYPVKACWLMTNSQNPLGFTLTPQKKAQLVALLNQYNVTL
                     IEDDVYSELYFGREKPLPAKAWDHHDGVLHCSSFSKCLVPGFRIGWVAAGKHARKIQR
                     LQLMSTLSTSSPMQLALVDYLSTRRYDAHLRRLRRQLAERKQRAWQALLRYLPAEVKI
                     HHNDSGYFLWLELPEPLDAGELSLVALTHHISIAPGKMFSTGENWSRFFRFNTAWQWG
                     EREEQAVKQLGKLIQERL"
     gene            6454..7599
                     /locus_tag="PN977_002837"
     CDS             6454..7599
                     /locus_tag="PN977_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDI6042855.1"
                     /translation="MSKIFARSSLCALTMTIMTAHAAEPPTNLDKPEGRLDIIAWPGY
                     IERGQTDNQYDWVTQFEKETGCAVNVKTAATSDEMVSLMTKGGYDLVTASGDASLRLI
                     MGKRVQPINTALIPNWKALDPRVVKGDWFNVGGKVYGTPYQWGPNLLMYNTKTFPTPP
                     NSWQVVFVEQNLPDGKSNKGRVQAYDGPIYIADAALFVKATQPQLGISDPYQLTEEQY
                     QAVLKVLRDQHSLIHRYWHDTTVQMSDFKNEGVVASSAWPYQANALKAEGQPVATVFP
                     KEGVTGWADTTMLHSEAKHPVCAYKWMNWSLTPKVQGDVAAWFGSLPVVPQGCKASPL
                     LGEKGCETNGFNYFDKIAFWKTPIAEGGKFVPYSRWTQDYIAIMGGR"
     gene            7617..8630
                     /locus_tag="PN977_002838"
     CDS             7617..8630
                     /locus_tag="PN977_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDI6042856.1"
                     /translation="MTYAVEFDNVSRLYGDVRAVDGVSIAIKDGEFFSMLGPSGSGKT
                     TCLRLIAGFEQLSGGAISIFGKPASNLPPWERDVNTVFQDYALFPHMSILDNVAYGLM
                     VKGVDKKQRHAMAQEALEKVALGFVQQRKPSQLSGGQRQRVAIARALVNEPRVLLLDE
                     PLGALDLKLREQMQLELKKLQQSLGITFIFVTHDQGEALSMSDRVAVFNNGRIEQVDS
                     PRDLYMRPRTPFVAGFVGTSNVFDGLMAEKLCGMTGSFALRPEHIRLNTPGELQANGT
                     IQAVQYQGAATRFELKLNGGEKLLVSQANMTGEELPATLTPGQQVMVSWSRDVMVPLV
                     EER"
     gene            8631..9572
                     /locus_tag="PN977_002839"
     CDS             8631..9572
                     /locus_tag="PN977_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDI6042857.1"
                     /translation="MAMNVLQSPSRPGLGKVSCFFWRNPGLGLFLLLLGPLMWFGIVY
                     FGSLLTLLWQGFYTFDDFTMSVTPELTLANIRALFNPANYDIILRTLTMAVAVTIASA
                     ILAFPMAWYMARYTSGKMKAFFYIAVMLPMWASYIVKAYAWTLLLAKDGVAQWFLQHL
                     GLEPLLTAFLTLPAVGGNTLSTSGLGRFLVFLYIWLPFMILPVQAALERLPPSLLQAS
                     ADLGARPRQTFRYVVLPLAIPGIAAGSIFTFSLTLGDFIVPQLVGPPGYFIGNMVYSQ
                     QGAIGNMPMAAAFTLVPIVLIALYLAFVKRLGAFDAL"
     gene            9562..10356
                     /locus_tag="PN977_002840"
     CDS             9562..10356
                     /locus_tag="PN977_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDI6042858.1"
                     /translation="MHSERAPFFLKLAAWGGVVFLHFPILIIAAYAFNTEDAAFSFPP
                     QGLTLRWFSVAAQRSDILDAVTLSLKVATLATLIALVLGTLAAAALWRRDFFGKNAIS
                     LLLLLPIALPGIVTGLALLTAFKTINLDPGFFTIVVGHATFCVVVVFNNVIARFRRTS
                     WSLVEASMDLGANGWQTFRYVVLPNLSSALLAGGMLAFALSFDEIIVTTFTAGHERTL
                     PLWLLNQLGRPRDVPVTNVVALLVMLVTTLPILGAWWLTREGDNGQ"
     gene            10378..11802
                     /gene="patD"
                     /locus_tag="PN977_002841"
     CDS             10378..11802
                     /gene="patD"
                     /locus_tag="PN977_002841"
                     /EC_number="1.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminobutyraldehyde dehydrogenase"
                     /protein_id="HDI6042859.1"
                     /translation="MQHKLLINGELVSGEGEKQPVYNPATGDVLLEIAEASAEQVDAA
                     VRAADAAFAEWGQTTPKARAECLLKLADVIEENGQVFAELESRNCGKPLHSAFNDEIP
                     AIVDVFRFFAGAARCLNGLAAGEYLEGHTSMIRRDPLGVVASIAPWNYPLMMAAWKLA
                     PALAAGNCVVLKPSEITPLTALKLAELAKDIFPAGVINVLFGRGKTVGDPLTGHPKVR
                     MVSLTGSIATGEHIISHTAPSIKRTHMELGGKAPVIVFDDADIEAVVEGVRTFGYYNA
                     GQDCTAACRIYAQKGIYDTLVEKLGAAVATLKSGAPDDDSTELGPLSSLAHLERVSKA
                     VEEAKATGHIKVITGGEKRKGNGYYYAPTLLAGALQDDAIVQKEVFGPVVSVTLFDNE
                     EQVVNWANDSQYGLASSVWTKDVGRAHRVSARLQYGCTWVNTHFMLVSEMPHGGQKLS
                     GYGKDMSLYGLEDYTVVRHVMVKH"
     gene            complement(11899..11994)
                     /gene="yncL"
                     /locus_tag="PN977_002842"
     CDS             complement(11899..11994)
                     /gene="yncL"
                     /locus_tag="PN977_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response membrane protein YncL"
                     /protein_id="HDI6042860.1"
                     /translation="MNVSSRTVVLINVFAAVGLFTLISMRFGWFI"
     gene            12189..12362
                     /gene="ortT"
                     /locus_tag="PN977_002843"
     CDS             12189..12362
                     /gene="ortT"
                     /locus_tag="PN977_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orphan toxin OrtT"
                     /protein_id="HDI6042861.1"
                     /translation="MSLYQHMLVFYAVMAAIAFLITWFLSHDKKRIRFLSAFLVGATW
                     PMSFPVALLFSLF"
     gene            12448..12681
                     /locus_tag="PN977_002844"
     CDS             12448..12681
                     /locus_tag="PN977_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcY family protein"
                     /protein_id="HDI6042862.1"
                     /translation="MSHLDEVIARVDAAIEESVIAHMNELLIALSDDAELSREDRYTQ
                     QQRLRTAIAHHGRKHKEDMEARHEQLTKGGTIL"
     gene            complement(12682..13131)
                     /locus_tag="PN977_002845"
     CDS             complement(12682..13131)
                     /locus_tag="PN977_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HDI6042863.1"
                     /translation="MNQSLTLAFLIAAGIGLVVQNTLMVRITQTSSTILIAMLLNSLV
                     GIVLFVSILWFKQGMAGFGELVSSVRWWTLIPGLLGSFFVFASISGYQNVGAATTIAV
                     LVASQLIGGLVLDIFRSHGVPLRALFGPICGAILLVVGAWLVARRSF"
     gene            complement(13128..13646)
                     /gene="mddA"
                     /locus_tag="PN977_002846"
     CDS             complement(13128..13646)
                     /gene="mddA"
                     /locus_tag="PN977_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine sulfoximine/L-methionine sulfone
                     acetyltransferase"
                     /protein_id="HDI6042864.1"
                     /translation="MSIRFARKADCAAIAEIYNHAVLYTAAIWNDQTVDADNRIAWFE
                     ARTLAGYPVLVSEQDGVVTGYASFGDWRSFDGFRHTVEHSIYVHPDHQGKGLGRKLLS
                     RLIDEARDCGKHVMVAGIESQNQASLHLHQSLGFVVTAQMPQVGTKFGRWLDLTFMQL
                     QLDERSEPDAIG"
     gene            13827..14864
                     /gene="curA"
                     /locus_tag="PN977_002847"
     CDS             13827..14864
                     /gene="curA"
                     /locus_tag="PN977_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415966.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent curcumin/dihydrocurcumin
                     reductase"
                     /protein_id="HDI6042865.1"
                     /translation="MGQQKQRNRRWVLASRPHGAPVPENFRLEEDDVATPGEGQVLLR
                     TVYLSLDPYMRGRMSDEPSYSPPVDIGGVMVGGTVSRVVESNHPDYQPGDWVLGYSGW
                     QDYDISSGDDLVKLGDHPQNPSWSLGVLGMPGFTAYMGLLDIGQPKEGETLVVAAATG
                     PVGATVGQIGKLKGCRVVGVAGGAEKCRHATEVLGFDVCLDHHADDFAEQLAKACPKG
                     IDIYYENVGGKVFDAVLPLLNTSARIPVCGLVSSYNATELPPGPDRLPLLMATVLKKR
                     IRLQGFIIGQDYGHRIHEFQQEMGQWVKEGKIHYREQITNGLENAPQTFIGLLNGKNF
                     GKVVIRVADDD"
     gene            15063..15728
                     /gene="mcbR"
                     /locus_tag="PN977_002848"
     CDS             15063..15728
                     /gene="mcbR"
                     /locus_tag="PN977_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid/biofilm transcriptional regulator
                     McbR"
                     /protein_id="HDI6042866.1"
                     /translation="MPGTEKIPHISLTMQVENDLKHQLSIGALKPGARLITKNLAEQL
                     GMSITPVREALLRLVSVNALSVAPAQAFTVPEVGKCQLDEINRIRYELELMAVALAVE
                     NLTPQDLAEFHDLLEKLQQAQEKGDMEQIINANRLFRLAIYHRSNMPILCEMIEQLWV
                     RMGPGLHYLYETINPAELRERIENYHLLLAALKAKDKEGCRHCLAEIMQQNIAILYQQ
                     YNR"
     gene            complement(15764..17866)
                     /locus_tag="PN977_002849"
     CDS             complement(15764..17866)
                     /locus_tag="PN977_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HDI6042867.1"
                     /translation="MKIFSVRQTVLPALLVLSPVVFAADEQTMIVSAAPQVVSELDTP
                     AAVSVVDGEEMRLATPRINLSESLTGVPGLQVQNRQNYAQDLQLSIRGFGSRSTYGIR
                     GIRLYVDGIPATMPDGQGQTSNIDLSSVQNVEVLRGPFSALYGNASGGVMNVITQTGQ
                     QPPTIEASSYYGSFGSWRYGLKATGATGDGTQPGDVDYTVSTTRFTTHGYRDHSGAQK
                     NLANAKLGVRIDEASKLSLIFNSVDIKADDPGGLTEAEWKANPQQAPRAEQYDTRKTI
                     KQTQAGLRYERSLSAQDDMSVMMYAGERETTQYQSIPMAPQLNPSHAGGVITLQRHYQ
                     GIDSRWTHHGELGVPVTFTTGLNYENMSENRKGYNNFRLNSGVPEYGQKGELRRDERN
                     LMWNVDPYLQTQWQLSEKLSLDAGVRYSSVWFDSNDHYVTPGNGDDSGDASYHKWLPA
                     GSLKYAMNDAWNIYLAAGRGFETPTINELSYRADGQSGMNFGLKPSTNDTIEIGSKTR
                     IGDGLLSLALFQTDTDDEIVVDSSSGGRTTYKNAGKTRRQGAELAWDQRFAGDFRVKA
                     SWTWLDATYRSNVCNEQDCNGNRMPGIARNMGFASIGYVPEEGWYAGTEARYMGDIMA
                     DDENTAKAPSYTLVGLFTGYKYNYHNLTVDLFGRVDNLFDKGYVGSVIVNESNGRYYE
                     PAPGRNYGVGMNIAWRFE"
     gene            18108..19169
                     /locus_tag="PN977_002850"
     CDS             18108..19169
                     /locus_tag="PN977_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="HDI6042868.1"
                     /translation="MHLRHLFSSRLRGSLLLGSLLVASSFSTQAAEEMLRKAVGKGAY
                     EMAYSQQENALWLATSQSRKLDKGGVVYRLDPVTLEVTQAIHNDLKPFGATINNTTQT
                     LWFGNTVNSAVTAIDAKTGEVKGRLVLDERKRTEEVRPLQPRELVADDATNTVYISGI
                     GKESVIWVVDGENIKLKTAIQNTGKMSTGLALDSKGKRLYTTNADGELITIDTADNKI
                     LSRKKLLDDDKEHFFINISLDTTNQRAFITDSKAAEVLVVDTRNGNILAKVAAPESLA
                     VLFNPARNEAYVTHRQAGKVSVIDAKSYKVVKTFDTPTHPNSLALSADGKTLYVSVKQ
                     KSTKQQEATQPDDVIRIAL"
     gene            complement(19282..20781)
                     /gene="ansP"
                     /locus_tag="PN977_002851"
     CDS             complement(19282..20781)
                     /gene="ansP"
                     /locus_tag="PN977_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparagine permease"
                     /protein_id="HDI6042869.1"
                     /translation="MSKHDTDTSDQHAAKRRWLNAHEEGYHKAMGNRQVQMIAIGGAI
                     GTGLFLGAGARLQMAGPALALVYLICGLFSFFILRALGELVLHRPSSGSFVSYAREFL
                     GEKAAYVAGWMYFINWAMTGIVDITAVALYMHYWGAFGGVPQWVFALAALTIVGTMNM
                     IGVKWFAEMEFWFALIKVLAIVTFLVVGTVFLGSGQPLDGNTTGFHLITDNGGFFPHG
                     LLPALVLIQGVVFAFASIEMVGTAAGECKDPQTMVPKAINSVIWRIGLFYVGSVVLLV
                     MLLPWSAYQAGQSPFVTFFSKLGVPYIGSIMNIVVLTAALSSLNSGLYCTGRILRSMA
                     MGGSAPSFMAKMSRQHVPYAGILATLVVYVVGVFLNYLVPSRVFEIVLNFASLGIIAS
                     WAFIIVCQMRLRKAIKEGKAADVSFKLPGAPFTSWLTLLFLLSVLVLMAFDYPNGTYT
                     IAALPIIGILLVIGWFGVRKRVAEIHSTAPVVEEDEEQQEIVFKPETAS"
     gene            21048..21665
                     /locus_tag="PN977_002852"
     CDS             21048..21665
                     /locus_tag="PN977_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HDI6042870.1"
                     /translation="MIKVYGVPGWGSTISELMLTLADIPYQFVDVSGFDREGTSRELL
                     KTLNPLCQIPTLALENDEIMTETAAIALMVLDRRPDLAPPLGRVERQQFQQLLVWLVA
                     NVYPTFTFADYPERWAPDAPEQLKNNVIEYRKSLYIWLNSQLTAEPYVFGEQLTLVDC
                     YLCTMRTWGPGHEWFQDNAPNINAIADAVCQIPKLKGILKRNGII"
     gene            complement(21666..22715)
                     /locus_tag="PN977_002853"
     CDS             complement(21666..22715)
                     /locus_tag="PN977_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000236738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-associated sensor domain-containing
                     protein"
                     /protein_id="HDI6042871.1"
                     /translation="MVIRGMTISLPWFAFINVSFALIILLRRVLFNDLTPPWLNEKSL
                     IHSIDISATGILLICSGLLLIPRQKTLPIQVLLVALSLLWSWCSYHFIAYWTLQFAYP
                     LCVLLMLSGVIALYFHTPSLLAFVIPLWFTTPIASLMLNQQINIHFAVVWCIFSLALY
                     GGRLILLRWFEEAWVQNSYNNQLINRLDALAHRDPLTGIANRRAMNSILHDAIDNGGS
                     FALIMLDVDFFKRYNDTYGHPAGDRCLIQVADALQRSTRQSEDVVVRYGGEEFVIILF
                     NATLPEAETVAARVKQELKLAAIPHQASAVNAFVTVSQGITCSAPAKTAEQIISDADA
                     ALYRAKESGRNRWEK"
     gene            complement(22810..24786)
                     /locus_tag="PN977_002854"
     CDS             complement(22810..24786)
                     /locus_tag="PN977_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020078314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HDI6042872.1"
                     /translation="MQLNHIVKSLLITGLFTTSSLPLLAAEAPKDATAATQQANNLLY
                     NQLPFSDNTDFTDAHKGFVAPIPQDVIKGEKGNVIWDPQQYAFIKEGDKAPDTVNPSL
                     WRQAQLINISGLFEVTEGVYQIRNLDLSNMTIIEGKEGITIVDPLVSAETAKVGMDLY
                     YKNRDKKPVVAVIYTHSHVDHYGGVRGVIDEADVKSGKVKIYAPAGFLEEAVSENIMA
                     GNVMSRRASYMYGNLLKPDVKGQVGAGLGTTTSAGTVTLIAPTNYITKTGQKETIDGL
                     TYDFLMAPGSEAPSEMLWFIEEKKLIETAEDVTHTLHNTYSLRGAKIRQPLPWSKYIN
                     EALNLWGDKAEIILAQHHWPTWGNDNVVKLLKSQRDLYRYINDQTLRMANQGMTRDEI
                     AANFKLPSSLANTWANRGYYGSVSHDVKATYVLYLGWFDGNPATLDELPPEEGAKKFV
                     EYMGGADAILQKAKQDYDQGNFRWVAQVVSKVVFTDPNNQAARNLEADALEQLGYQAE
                     SGPWRNFYLTGAQELRNGVQKLPTPNTASPDTVRAMTPEMFFDYLAVHINGEKAADAK
                     TVLNFDFGEDGGTYKVELENGVLNHTAGVEASDADATITLSRDVLNKIVLKEETLKEA
                     TAKEDVKITGNAEKLNELLGYMDNFEFWFNIVTP"
     gene            25091..25318
                     /gene="pptA"
                     /locus_tag="PN977_002855"
     CDS             25091..25318
                     /gene="pptA"
                     /locus_tag="PN977_002855"
                     /EC_number="5.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tautomerase PptA"
                     /protein_id="HDI6042873.1"
                     /translation="MPHIDIKCFPRELDEQQKAALAADITDVIIRHLNSKDSSISIAL
                     QQIQPESWQAIWDTEIAPQMETLIKKPGYSM"
     gene            complement(25322..>25525)
                     /locus_tag="PN977_002856"
     CDS             complement(25322..>25525)
                     /locus_tag="PN977_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415979.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase like domain protein"
                     /protein_id="HDI6042874.1"
                     /translation="AWMECRLLPATSAQEKYDSLFGEVVSAAADVRVFVEGRWQFDDD
                     KLNTLHHLGAGTFVTSGKRVTAG"
BASE COUNT         6588 a         6210 c         6410 g         6317 t
ORIGIN      
        1 caacggcagc ggccagccaa cgttgatctt tatccggatg cgcttccggg ctttggcaaa
       61 tagccgcggt gattgccgca ataccgacgg aataaacgcc gaaaggggaa aaaaccagtg
      121 ccagcaatcc agtaaataca attaatggcg aaacaggagc cgaatatccg gctgctttca
      181 ttgctgcgat acccggtgcg ttttgcgatg ccatcgtcac cagaaaaagg gggagtgcaa
      241 cgctcaggct gtgagcaaac gaaaaatcag ggctaatata agtggggaga acgggtttaa
      301 agacaacatc agttgtgaca atgtcacctt gcgcgataac gatcacgacc ccaataatca
      361 tcgcggcaat taccgcatag cgcggcgcaa cggccctggt tgccagccat accagcaaca
      421 tactgccaca caacgtaaat tgaccatcca ggctcgcaaa cgcctgtaaa ccaaagcgta
      481 ataaaatccc ggcaagcatt gccgccgcaa gcgagtgcgg aataatgcgc atcagatgag
      541 caaagagtcc cgttatgccg cagaggacta ttaacgcgtt ggtgacaata aaaacgccga
      601 tggtttcgtt aagtgttagt ccctgcaatc cggtgaccaa caaagccgcg ccaggcgttg
      661 accatgcggt gagaacaggt acgcgatacc ataatgtcag agccagcgta ctgacgccca
      721 ttgccagccc cagcgccgtc atccagccag agatttgtgc agtggtggct ccggcgacaa
      781 tcgctgcttg ccagattatt gccgctgaac tggcgtagcc aattaatacc gccagaaacc
      841 ccgccagtag cgtgggtgga ggaatagaaa acagacgcat agtcacctcg tgcgttatag
      901 cgtacaggca ccgtagcatt tgtccgttat aacgcacaag tgataaactt ccgttttgcc
      961 ggaggagtcg catggaaaat ctcgctcgct ttttatccac cacacttaaa caactacgcc
     1021 agcagcgcgg ctggagtctt tcgcgactgg ccgaagcgac gggcgtttct aaagcaatgt
     1081 tagggcagat tgagcgtaat gaatccagcc cgacggtcgc gacattatgg aaaattgcca
     1141 ccgggttgaa cgtaccattt tcaacattta tttctccgcc gcagtccgct acaccttcag
     1201 tttatgatcc acaacagcag gcgatggtga tcacttcgct gtttccttat gatccacaac
     1261 tctgctttga acacttctcg attcagatgg catctggtgc aataagcgaa tcgacgcccc
     1321 atgagaaggg ggtaatcgaa catgtcgtcg tcattgatgg acaacttgat ctgtgcgttg
     1381 atggtgaatg gcagagcctt aattgtgggg aaggcgttcg atttgctgca gacgtcacgc
     1441 atatctatcg taatggtggg gagcaaaccg tacattttca ttccctcatc cattacccgc
     1501 gcagttaagc gggaaaacta tttcgcaacg tcgcgcttgt ggctaaaata gcagccattt
     1561 ttcagctact ggataagaat gtgaccgtat cttctcatcg acttgaactg ttaagcccgg
     1621 cacgcgatgc cgccattgcc cgcgaagcta ttttgcacgg tgccgatgct gtttatatcg
     1681 gcggacctgg ttttggtgcc cgtcataatg ccagtaatag cctgaaagat attgccgagt
     1741 tggtgccgtt tgcccatcgt tatggtgcaa aaattttcgt cacgcttaac accattttgc
     1801 atgatgatga gctggaaccc gcacaacggc tgattaccga cctctatcag accggcgtcg
     1861 atgcgctgat tgttcaggat atggggattc tggaacttga tattccgccg attgaactgc
     1921 acgccagtac gcagtgcgac attcgtacag tagaaaaagc gaagttcctc tctgatgttg
     1981 gcttcacgca gattgtgctg gcgcgagagc tgaatcttga gcagatccgc gcgattcacc
     2041 aggccacgga cgcgaccatt gaattcttta ttcatggcgc actgtgcgtg gcctattcgg
     2101 gtcagtgcta catttctcat gcgcaaacag ggcgtagcgc caaccgtggc gattgctcgc
     2161 aggcgtgccg tttgccatac acattgaaag acgatcaggg gcgggtggtt tcctatgaaa
     2221 aacatctgct gtcgatgaaa gataacgatc agactgccaa cctcggcgcg ctgattgatg
     2281 ctggcgtacg ctccttcaag attgaagggc gttacaaaga tatgagctac gtgaagaata
     2341 tcaccgccca ttatcgtcag atgctggatg ccattattga agaacgtggc gatctagcgc
     2401 gcgcttcatc aggtcgtact gaacatttct ttgttccatc gacggaaaag actttccacc
     2461 gtggtagcac agattatttt gtgaatgccc gtaaaggcga tattggcgcg ttcgattcgc
     2521 cgaaatttat cggcctgccg gtgggtgaag tattgaaagt ggcgaaagat catctcgatg
     2581 ttgccgtttc cgaaccactg gcaaatggcg atggcctgaa cgtgatgatt aaacgtgaag
     2641 tcgtcggttt tcgtgccaat acggtagaga aaaccggaga aaatcagtac cgcgtctggc
     2701 ccaatgaaat gccagcagag ttgcacaaaa ttcgcccgca tcacccacta aaccgtaatc
     2761 ttgatcatga ctggcagcag gcactgacaa aaacctctag cgaacgtcgg gtggcggtag
     2821 acattgaact gggcggctgg caggaacaac tgattctgac cctcaccagt gaagagggtg
     2881 tcagcatcac gcatacgctg gacgggcagt tcgacgaagc caataacgcc gaaaaagcaa
     2941 tgaacaatct aaaggatggt ctggcaaaac tggggcaaac catctattac gcccgcgatg
     3001 tgcaaattaa tttgccgggt gcgctgtttg taccaaacag tctgctaaac cagttccgcc
     3061 gtgaagccgc cgatatgctt gatgctgcgc gtcttgccag ttaccagcgc ggcagccgta
     3121 aaccggttgc tgatcctgcg ccggtttatc cgcaaacgca tctgagtttc ctcgcgaacg
     3181 tatacaacca gaaagcgcgt gaattttatc atcgctatgg tgtgcaactg attgacgcgg
     3241 cgtatgaggc acatgaagag aagggcgaag tcccggtgat gatcaccaag cattgtctgc
     3301 gctttgcctt caatctgtgc ccgaaacagg cgaaaggcaa tatcaaaagc tggaaggcaa
     3361 cgccaatgca actggttaac ggtgatgaag tgttaacgct gaaatttgat tgccgcccat
     3421 gcgagatgca cgtcattggc aaaatcaaaa atcacatact gaaaatgccg ttaccgggaa
     3481 gcgtagtggc atccgtaagt ccggatgatc tgctgaaaac attgccgaag cgaaaagggt
     3541 aaaacgtcag ttttcgggtt actcaccact catcaaatct gcatgatatt gcctggcggg
     3601 taaggcgtca tgccgcatcc ggcaacaaat gactcaacgc tgtcccgctt ctggtttgcg
     3661 cgatttttgc cagtaaaaat gctcgcgcaa accttccgcc gactcttccg ccacagcacg
     3721 caattcatcg ctgtccgctt catgacgcag ctgatgatcc acattcttta cccacataaa
     3781 ttcatgtcct ttgtgccctg ccatgagttg tcctgagaac agtgcacacg ttaataagac
     3841 aaccgataac gccttcgaaa acatcccgcc acctttttct aaccttttgc cgctatgatg
     3901 ccaatcgttt cttgaggtta ttattcagtt ttgcaaatta gcgcaaagaa attctggaat
     3961 cttccttcct gattttgcat tgcattctgc cgttgcggcg atttagtgct attttcgagc
     4021 aaattacaca cggaggtaaa cactaatgtt tactttgttg atatactcag cggcagggag
     4081 gcgatgtgaa acaaagcgag ttcagacgtt ggctcgaatc tcagggcgtc gatgtagcga
     4141 atggcagcaa ccatttaaaa ctgaggtttc atgggaggcg cagtgtcatg ccgcgtcacc
     4201 cctgcgatga gattaaagaa ccattgcgta aagcaatcct gaaacaactc ggtttgagtt
     4261 aatcgccaat taaaaaggtt aatgacatgc gagagacagt cgaaattatg cgttatcccg
     4321 tcactcttac acccgcgccg gaaggcggtt atatggtttc ttttgtggat atccctgaag
     4381 cgttgaccca gggcgaaaca gttgctgaag cgatggaagc ggcaaaagat gctttactga
     4441 ccgcatttga tttttatttt gaagataacg agcttatccc gttaccttcg ccattaaaca
     4501 gtcacgatca ctttattgaa gtgcctttga gcgtcgcctc taaagtattg ctgttaaatg
     4561 cttttttaca gtcagaaatc actcagcaag agttagccag gcgaattggc aaacctaaac
     4621 aggagattac tcgcctattt aatttgcacc atgcgacaaa aatcgacgcc gtccagctcg
     4681 cggctaaggc gcttggcaaa gagttatcac tggtgatggt ttgataacag ttaatgaaaa
     4741 gttgtcattt ttaacaactg atatagactg ccgaatcacc tgcacataat aacgattcga
     4801 taatgaaaaa ataccagcag cttgcagaac aattacgcga gcagattgcg tcgggtatct
     4861 ggcaacccgg agatcgtttg ccttcgttgc gtgaccaggt ggcactttct ggcatgagct
     4921 ttatgaccgt gagccatgcc tatcagttgc tcgaaagtca gggatatatt atcgcacgac
     4981 cgcagtcggg ttattacgtt gcgccacagg caataaaaat gccgaaagcg ccagtcattc
     5041 cagtcactcg agatgaagca gtcgatatca acacttatat ttttgatatg ttgcaggcca
     5101 gtcgcgatcc gtcggtcgtt ccgtttgcct cggcctttcc cgacccgcga cttttccccc
     5161 tccaacaact aaaccgctcg ctggcgcagg taagcaaaac cgccacagcg atgagcgtga
     5221 ttgaaaactt accgccagga aacgcagaac tgcgtcaggc tattgctcgt cgctatgcct
     5281 tacagggcat cactatttct ccagatgaaa ttgccattac cgccggggcg ttagaggcat
     5341 taaacctcag tttgcaagtg gtaactgaac cgggcgattg ggtgatagta gagaatcctt
     5401 gtttctacgg cgctttgcag gcgctggagc ggttacggct gaaggcgtta tcggtggcga
     5461 cggacgttaa agaagggatc gatcttcagg cgctggaact ggcgttgcag gagtatccgg
     5521 tgaaagcgtg ctggctgatg actaatagcc agaatccact cggatttacc ttaacaccgc
     5581 aaaaaaaagc acaactggtg gcgttgctca atcagtacaa cgtaacgctg attgaagatg
     5641 acgtttacag cgaactttat tttggacggg aaaaaccgct gcctgcgaaa gcgtgggatc
     5701 accacgatgg tgttttgcat tgctcttcgt tttcgaaatg tctggtgcct ggttttcgta
     5761 ttggttgggt cgccgccgga aaacatgcac gtaaaattca acgcttgcag ttgatgagta
     5821 cgctttccac cagctcaccg atgcagcttg cgctggtgga ttacctttct actcgccgat
     5881 acgatgctca tcttcgtcgt ctgcgtcgcc agcttgcgga acgtaaacaa cgcgcctggc
     5941 aggctttgct gcgttatctg cctgcggaag tcaaaattca tcacaatgac agcggttatt
     6001 ttctctggct ggagctcccc gagccgttag atgcaggaga attaagcctg gtggcactga
     6061 cgcatcatat cagtattgcg ccgggtaaaa tgttttctac cggtgaaaac tggtcacgtt
     6121 tttttcgttt taataccgcg tggcagtggg gagaacgtga agaacaggcg gtaaaacaat
     6181 taggcaaact tattcaagaa cggctgtaat agcgtttaat ttaattcctc tcaggatggg
     6241 taatatgaat ttcgaatagc agtcatattt cctaactcct tgtctatact ccagaatata
     6301 accttacaga gggcataatg cgcggtagct cacaacctga ttaaattttc tcaggggcga
     6361 aggtgtgcct gctagccgcc gtctatggtt aaacaaggag atatttttac ggcacggcgg
     6421 ctgaacaatt aattacgaca ggagtaagac cttatgagca agatttttgc ccgcagcagc
     6481 ctgtgtgcgc tcaccatgac aataatgacc gctcacgccg ccgaaccgcc taccaattta
     6541 gataaaccgg aagggcgact ggatattatc gcctggccgg gatacatcga acgcggacaa
     6601 actgataatc aatacgactg ggtaacgcaa ttcgaaaaag agacaggctg cgcggtgaat
     6661 gtgaaaaccg ccgcgacttc cgatgaaatg gtcagtctga tgaccaaagg gggttacgat
     6721 ctggttacgg catccggcga tgcctcgctg cgcctgatta tgggcaaacg cgtgcagccg
     6781 attaataccg cattgattcc caactggaaa gcgctcgatc cgcgcgtggt taaaggcgac
     6841 tggtttaacg ttggcggcaa agtttacggc acaccttacc aatgggggcc gaacctgctg
     6901 atgtacaaca ctaaaacctt cccgacgccg ccgaatagct ggcaagtggt ttttgttgag
     6961 cagaatctgc cggacggcaa gagcaataaa ggccgcgttc aggcttatga tggccctatc
     7021 tacattgcgg acgctgcgtt gttcgttaaa gccactcagc cgcagttggg catcagcgat
     7081 ccgtatcaac tcaccgaaga acagtaccag gcggtgctga aagtgctgcg cgatcaacat
     7141 agtttgatcc atcgctactg gcatgacact accgtgcaaa tgagcgattt caaaaacgag
     7201 ggtgtagttg cttccagtgc atggccctat caggccaacg ccctgaaagc cgaaggccag
     7261 cctgtcgcta ccgttttccc gaaagagggc gttaccggtt gggctgacac caccatgcta
     7321 catagcgaag cgaaacatcc ggtttgcgcc tacaaatgga tgaactggtc attaacccca
     7381 aaagtgcagg gcgatgtggc ggcctggttt ggctcgctac cagtagtgcc gcaagggtgt
     7441 aaagccagtc cgttattagg cgagaaaggt tgtgaaacaa acggttttaa ctatttcgat
     7501 aaaatcgcct tctggaaaac gcctatagca gaagggggca agtttgttcc ctacagtcgc
     7561 tggacgcagg attacattgc cattatgggt ggtcgctaac ttcgctgggg tgttttatga
     7621 cgtacgcagt ggagtttgac aacgtctcac ggttgtacgg tgacgtgcgc gcagtagatg
     7681 gcgtcagtat tgcgataaaa gatggtgagt tcttctctat gctggggccg tccggttccg
     7741 gcaaaaccac ctgcctgcgc ctgattgctg gattcgaaca actttccggc ggggctatct
     7801 ctatctttgg taaacccgcc agcaatctgc caccgtggga gcgggacgtg aatactgtct
     7861 tccaggacta cgcgctattt ccgcatatgt cgattcttga caacgtcgcc tacgggctga
     7921 tggtcaaagg cgtggataaa aaacagcggc atgcgatggc gcaagaggcg ctggagaaag
     7981 tggcgctggg ttttgttcag caacgtaaac cgtcacaact ttctggtggt cagcgccagc
     8041 gggttgctat cgccagagca ctggtgaatg aaccgcgcgt attgctgttg gatgaaccgc
     8101 tcggcgcact ggatctcaaa ttgcgtgagc agatgcagct ggaactgaaa aaactgcaac
     8161 agtctctcgg tatcactttt atcttcgtta cccacgatca gggcgaagcg ttatcgatgt
     8221 ccgatcgtgt ggcggttttc aataatggac gcattgagca ggtcgattcc ccgcgcgatc
     8281 tctatatgcg cccgcgcacg ccgtttgttg ccgggttcgt tggtacatcg aatgtttttg
     8341 atggactgat ggcagagaaa ctttgtggca tgacgggaag cttcgccctg cgaccggaac
     8401 atatccgcct caacacccct ggtgaactgc aggccaatgg cacgatccag gcggtgcaat
     8461 atcagggcgc ggcaactcgt tttgaactga aattgaacgg cggtgaaaaa ctgcttgtga
     8521 gtcaggccaa tatgacaggc gaagaactgc ctgccacgct cacgcccgga caacaggtga
     8581 tggtttcctg gtcgcgtgat gtgatggtgc cgctggttga ggagaggtga atggcgatga
     8641 atgtattgca atcaccttca cgtccaggtc tgggtaaggt gtcctgtttc ttctggcgta
     8701 atccggggct ggggctgttt ttactgctgc ttggcccgct aatgtggttt ggcattgtct
     8761 atttcggctc gctgctgacg ctgttatggc agggctttta tacctttgac gatttcacca
     8821 tgtcggtaac gccggaactg acgctggcga atatccgtgc gctgtttaat ccggcgaatt
     8881 acgacatcat tctccgcacg ctgactatgg ctgtggcagt cactatcgcc agcgccattc
     8941 tggcttttcc aatggcgtgg tatatggcgc gctataccag cgggaaaatg aaagcgtttt
     9001 tttatattgc ggtaatgttg ccgatgtggg cgagctacat tgttaaagcc tatgcctgga
     9061 cgttattgct ggcaaaagat ggcgtggctc agtggttttt acaacatctt gggctggaac
     9121 cactgctgac tgcgttcctt acattacctg cggtgggcgg caatacgctg tcaacttccg
     9181 ggctggggcg ctttctggtg tttctctata tctggctgcc gttcatgatc ctcccggtgc
     9241 aggcggcgct tgagcgtttg ccgccgtcat tgttgcaggc gtcagctgat ctcggcgcac
     9301 gtccacgaca aacctttcgc tatgtggtgc tgccgctggc gatcccgggt attgccgctg
     9361 gctctatctt taccttctca ctcacactgg gcgattttat cgtcccgcag ctggttggcc
     9421 cgccaggata ttttatcggc aatatggttt attcccagca gggggcgatt ggcaatatgc
     9481 cgatggcggc ggcattcacc ctggtgccga ttgttctcat cgcactgtac ctggcgttcg
     9541 tgaaacgtct gggagcgttc gatgcactct gaacgcgcac cgtttttcct taaactggcg
     9601 gcctggggcg gcgtggtttt cctacatttt cccatcctga ttatcgccgc ttatgcgttt
     9661 aacactgaag atgcggcgtt tagttttcca ccgcaaggcc tgacgttgcg ctggtttagc
     9721 gtggcagcac agcgtagtga cattcttgat gccgtgacac tgtcacttaa ggtggcgacg
     9781 ctggcgacat taatcgcgct ggtgttaggg acgctggctg ctgccgcact atggcgacga
     9841 gactttttcg gtaaaaacgc catttcgttg ttactgctgc tgcccattgc gctgccgggc
     9901 attgtcactg gtctggcgct attaaccgct tttaaaacca tcaatctgga tccgggattt
     9961 ttcaccatcg tggtcggtca tgcgactttt tgcgtagtgg tggtgtttaa caatgtcatc
    10021 gcccgttttc gccgcacctc gtggagtctg gttgaggcgt caatggatct tggggctaat
    10081 gggtggcaaa ccttccgcta cgtagtgttg ccgaatctca gttcggcgct actggcagga
    10141 ggaatgctgg catttgcctt gtcgttcgat gaaatcatcg tgacgacctt tacggcaggt
    10201 catgaacgaa cgttaccgtt gtggttgctc aatcagcttg ggcgaccgcg tgatgtaccg
    10261 gtaaccaacg tggtggcact gctggttatg ttggtaacaa ccttgccgat cctgggggcc
    10321 tggtggctaa cccgcgaagg cgacaatggt caataaccac tgatacagga atatgctatg
    10381 caacataagt tactgattaa cggagaactg gttagcggcg aaggggaaaa acagcctgtc
    10441 tataatccgg caacggggga cgttttactg gagattgccg aggcatccgc agaacaggtg
    10501 gatgctgctg ttcgcgcggc agatgcagca tttgccgaat gggggcaaac cacgccaaaa
    10561 gcgcgtgcgg aatgcctgct gaaactggcg gatgttatcg aagaaaatgg tcaggttttt
    10621 gccgaactgg aatcccgtaa ttgtggcaaa ccgctgcata gtgcgttcaa tgatgaaatt
    10681 ccggcgattg tcgatgtttt tcgctttttc gcgggtgcgg cgcgctgtct gaatggtctg
    10741 gcggcaggtg aatatcttga aggtcatact tcgatgatcc gtcgcgatcc gttgggggtt
    10801 gtggcttcta tcgcaccgtg gaattatccg ctgatgatgg cagcgtggaa actggctccg
    10861 gcgctggcgg cagggaactg cgtagtgctt aaaccatcag aaattacccc gctgaccgcg
    10921 ttgaagttgg ctgagctggc gaaagatatc ttcccggcag gcgtgattaa cgtactgttt
    10981 ggcagaggca aaacggtggg cgatccgctg accggacatc ccaaagtgcg gatggtgtcg
    11041 ttgacgggct ctatcgccac cggcgagcac atcatcagcc ataccgcgcc gtccattaag
    11101 cgtactcata tggaacttgg tggtaaagcg ccagtgattg tttttgatga tgcggatatt
    11161 gaagcagtgg tcgaaggtgt acgtactttt ggctattaca atgctggaca ggattgtact
    11221 gcggcttgtc ggatctacgc gcaaaaaggc atttacgata cgctggtgga aaaactcggt
    11281 gctgcggtgg caacgttaaa atctggtgca ccggatgacg actctacgga gcttggacct
    11341 ttaagctcgc tggcgcatct cgaacgcgtc agcaaggcgg tagaagaggc gaaagcgaca
    11401 gggcacatca aagtgatcac tggcggtgaa aagcgcaagg gtaatggcta ttactatgcg
    11461 ccgacgctgc tggctggcgc attacaggac gatgccatcg tgcaaaaaga ggtatttggt
    11521 ccggtagtga gtgttacgct cttcgacaac gaagaacagg tggtgaactg ggcaaatgac
    11581 agccagtacg gacttgcatc ctcggtatgg acgaaagatg tgggcagggc gcatcgcgtc
    11641 agcgcgcggc tgcaatatgg ttgtacctgg gtcaataccc atttcatgct ggtaagtgaa
    11701 atgccgcacg gtgggcagaa actttctggt tacggcaagg atatgtcact ttatgggctg
    11761 gaggattaca ccgtcgtccg ccacgtcatg gttaaacatt aaggataata ttgcagatcg
    11821 taatagtatg agatgatctt gcgtactgtc agagcactga tttctctgac agtacgtgct
    11881 gcgtgcaaaa tcactacatc aaataaacca gccaaatctc atagagataa gagtaaataa
    11941 accaacagca gcaaagacat ttatcagtac tacagttcta ctggaaacat tcatttttgc
    12001 caccttttat cattgcccct atgtgtatag tacgggttca gaaaatccgc aataatactc
    12061 attctgaaga tggataaggg caagttgctg ttagatgatt ttttctcaaa cgggtgaaaa
    12121 aacttgatgc acgtcaaaaa atgacgcata tttgcgcgcg ttttattcat ctggctggac
    12181 gcccgtacat gtctctctat caacacatgc ttgtttttta tgcggttatg gcagcaatcg
    12241 catttcttat cacctggttt ctttctcacg ataagaaacg catccgtttc ttaagcgcct
    12301 ttctggtggg ggcaacatgg ccgatgagtt tcccggtggc gctgttgttt tcactgtttt
    12361 aaatcttact cactggcttg acggcaccac gcagacttat atcatttgga tgaatcgata
    12421 aatttcacaa gtggctaatg agaaagtatg tcgcatctgg atgaagtcat cgcgcgcgtg
    12481 gatgccgcga ttgaagagag cgtcattgcc catatgaacg aattattaat cgccctgagc
    12541 gatgacgcgg agttaagtcg ggaagatcgc tacacccagc agcagcgttt gcgcacagcg
    12601 atcgcccatc acggtcgcaa gcataaagaa gatatggaag cgcgccatga acagttaacc
    12661 aaaggcggca ccatcctgtg attaaaatga gcgtctggct accaaccagg cgccaaccac
    12721 aagtaaaatt gcgccacaaa ttgggccgaa caacgcccgt agtggtacgc catgactgcg
    12781 gaagatatcc agaaccagac cgccaatcag ctggctggcg accagtactg caatagttgt
    12841 cgccgctccc acattctgat atccgctgat actggcgaag acaaaaaacg atcccagcaa
    12901 gccaggtata agtgtccacc agcgcacgct ggacaccagt tcgccaaacc ccgccatacc
    12961 ttgtttaaac cataaaatag aaacaaagag cacaatcccc accagtgagt tcagcaacat
    13021 ggcgatgaga atggtagagg aggtctgggt gatgcgcacc atcagcgtgt tttgcaccac
    13081 cagaccaatt ccggcggcaa tcaaaaaggc aagggtgagc gactgattca tccaatcgcg
    13141 tccggttcac tgcgttcgtc gagttgcaac tgcataaatg tcagatccag ccagcgccca
    13201 aatttagtgc ccacctgcgg catttgcgcg gtgacgacaa atcccagcga ctggtgcaga
    13261 tgcaaagacg cctgattttg cgattcgatc ccggcgacca tgacatgctt cccgcaatcc
    13321 ctcgcttcat caatcaatcg gcttaacaat ttacgcccaa gacctttgcc ctgatgatcg
    13381 ggatggacat aaatcgaatg ttccacggta tggcgaaaac catcgaaact acgccagtcg
    13441 ccaaacgagg catatcccgt cactacgcca tcttgttcac tcaccagcac cggataacca
    13501 gctaaagtcc gcgcttcaaa ccaggcaatg cgattatctg catccaccgt ttggtcattc
    13561 cagatagccg ccgtatacaa cacggcgtgg ttataaattt ccgcaatggc agcacagtcg
    13621 gctttgcggg caaaacggat ggacatgtct ggcctcgata aaataatgat gatgatgtta
    13681 tcaatactat cgaaaacagc ccgaagcaac ggattcctcg atgaaattat tgcgtgttgc
    13741 agaggggatg tgacggctgc aaacaaaacc aatcccgtga gcctgctact attgatggat
    13801 tcatgccaaa agcacaggag agcactatgg ggcaacaaaa gcagcgtaac cgccgttggg
    13861 ttctggcctc gcgtccgcat ggcgcgccgg ttccggagaa tttccgtctt gaagaagatg
    13921 atgtcgccac accgggtgaa ggtcaggtgt tactgcgcac agtttatttg tcactggacc
    13981 cgtatatgcg tggacgtatg agcgatgagc catcttattc accgccggtt gatattggcg
    14041 gcgtgatggt cggcggtacg gtgagccgtg tcgtggagtc gaatcatcct gattatcagc
    14101 ctggcgactg ggtgctgggc tacagtggat ggcaggacta tgacatatcc agtggtgatg
    14161 atctggtgaa acttggcgat catccgcaaa atccatcgtg gtcgctgggt gtgctgggga
    14221 tgccaggctt taccgcttat atggggctgc tggatatcgg tcagcctaaa gagggcgaaa
    14281 cgctggtggt agctgcggcg acaggaccag tgggggcgac ggtggggcaa atcggcaaac
    14341 ttaaaggttg cagggtggtg ggggttgccg gtggcgcgga aaaatgccgc catgccaccg
    14401 aagtgctggg ttttgatgtt tgccttgacc atcacgcgga tgattttgcc gaacaactgg
    14461 cgaaagcgtg cccaaaaggt attgatatct attatgaaaa cgttggtggt aaggtatttg
    14521 atgcggtgct accgttactc aatacatctg cgcgcattcc cgtctgcggc ttagtgagca
    14581 gctataacgc tacagagcta ccacccggtc cggatcgttt acccctgttg atggctacgg
    14641 tgctgaaaaa acgcattcga ttacaagggt ttattattgg tcaggattat ggtcaccgta
    14701 tccatgagtt tcagcaggag atgggacaat gggtgaaaga ggggaaaatt cactatcgtg
    14761 aacaaattac caacggtttg gagaacgccc cacagacgtt tattggcctg ctgaacggca
    14821 aaaacttcgg taaagttgtt attcgcgtcg cggatgatga ttaaagatac gacggcggca
    14881 taaagccgcc ataaaaatcc aggcaaaaat cttttttcca gctttaatta acttaccact
    14941 tctctgttct gtagctaata acaataaagg tgagtggata atgattattc agcttcatac
    15001 gccaggtaaa cgacttctgc tgtgctggca gagttggaac gaaaacacct gaaggaaata
    15061 acatgcctgg aacggaaaaa ataccgcata tcagtttaac tatgcaggtt gagaacgacc
    15121 tgaaacatca gcttagtatt ggcgcactaa aacctggcgc acgcctgatt actaaaaatc
    15181 tggcggagca attaggtatg agtattacac ccgtgcgtga agcattatta cgtctggttt
    15241 cggtgaatgc gctttctgtc gcaccagcgc aagcatttac agttccggaa gtggggaaat
    15301 gtcaattaga cgaaatcaat cggatccgct acgaactgga attaatggca gttgctctgg
    15361 ctgttgaaaa cctcaccccg caagacctgg cggaattcca tgatttactc gagaaattac
    15421 agcaggcgca agaaaagggc gacatggaac aaatcattaa tgcgaacaga ctatttcgct
    15481 tagcgattta tcatcgctca aatatgccca tcctgtgtga aatgattgag caactgtggg
    15541 tcaggatggg acctggttta cattatctct atgaaacgat taatccagcg gaattacggg
    15601 agcgtataga aaactatcat ctattactcg ccgcgttaaa agcaaaagac aaagagggat
    15661 gcagacattg tcttgctgaa attatgcaac aaaatattgc tattttatat cagcaataca
    15721 atcgttaaag taaatgcccg gcaaataccg ggcatcaagg taattactca aatcgccacg
    15781 caatattcat gccgacacca taatttcgcc cgggcgcagg ttcgtaatat cgaccgtttg
    15841 actcattgac aatgacagaa ccaacgtatc ctttatcgaa taaattatcg acacgaccaa
    15901 ataaatccac agtcaaattg tggtaattgt atttataccc ggtgaataag ccgacgagag
    15961 tataagacgg cgctttggcc gtattttcat catctgccat aatatcgccc atatatcgcg
    16021 cttccgtgcc tgcataccag ccttcttccg gtacataacc tatcgatgca aagcccatat
    16081 tacgggcgat ccctggcatc cggttaccgt tacaatcctg ttcattgcac acattgctgc
    16141 gataggtcgc atcaagccag gtccaggacg cttttacgcg aaaatctccc gcgaaacgtt
    16201 gatcccatgc cagttcagcg ccttgacgac gggtctttcc ggcattttta tatgtggtac
    16261 gcccaccgct actgctatcg acaacaattt catcatcggt gtcggtctgg aacaatgcga
    16321 gactcagcag cccatcacca atacgcgttt tactgccgat ctcaattgta tcgttggtag
    16381 atggttttaa accaaagttc ataccgcttt gcccatcagc gcgataagac agttcattaa
    16441 tcgtcggcgt ttcaaaacca cgcccggctg ccagatagat attccaggca tcgttcattg
    16501 catattttaa cgaaccggca ggtagccatt tgtgataact ggcatcaccg ctgtcatcgc
    16561 cgttacccgg agtaacgtaa tggtcgttgg aatcaaacca tacggagctg tagcgcacgc
    16621 cagcatccag cgacagtttt tcgctcagct gccactgtgt ctgtaaatag ggatcgacat
    16681 tccacatcag attgcgttcg tcgcgacgta actcaccttt ttgcccgtat tccggcacgc
    16741 cgctattcag gcggaagtta ttgtagccct tgcggttttc actcatgttt tcgtagttca
    16801 ggccagtagt gaacgtgacc ggaacaccca gctcgccatg gtgtgtccag cggctgtcta
    16861 tcccctgata atggcgttgc agagtaatca cgccgcccgc atgcgacgga ttaagttgag
    16921 gggccatcgg gattgactgg tactgggtcg tttctcgctc tccggcatac atcattacac
    16981 tcatatcatc ttgcgcgctc aggctacgtt catagcgcaa cccagcctga gtttgcttga
    17041 tggtttttcg cgtgtcgtac tgttcagcgc gtggcgcttg ttgcggattc gccttccatt
    17101 ctgcttcggt tagcccacct gggtcatctg ctttgatatc cacactattg aaaatcaggc
    17161 ttaatttgct ggcttcatca atgcgaacgc ccagtttggc attggctaaa tttttctgtg
    17221 cgccactatg gtcacgatag ccgtgggtcg taaaacgcgt ggttgagacg gtgtaatcga
    17281 catcgccagg ctgtgtgccg tcgcccgttg cgcctgttgc tttcagccca tagcgccagc
    17341 tgccaaaact gccgtagtaa ctactggctt caatggttgg tggctgttgt ccggtctggg
    17401 tgatgacatt cattacaccg ccagacgcgt tgccatacag agcagagaag gggccacgca
    17461 gcacttccac attttgcaca ctgcttaaat cgatgttgga tgtttgccct tgcccgtcgg
    17521 gcatggtggc gggaataccg tccacataca ggcgaatacc gcgaattccg taagtggagc
    17581 gggagccaaa tccgcgaatc gacagctgta aatcttgcgc atagttctgc cggttttgta
    17641 cctgcaaacc aggcacgcca gtcagtgatt cagacaagtt aatgcgcggt gttgccaggc
    17701 gcatctcctc gccatccacc acgcttactg ctgctggcgt atccagttct gaaaccacct
    17761 gcggcgcggc actgacaatc atggtctgtt catcagcggc aaaaacaacg ggggaaagga
    17821 caagcagtgc gggcaaaacg gtctgtcgga cggaaaaaat cttcatgaaa aaagccaggt
    17881 taagaatggg aaaacgccgt catggtaata aaattgtaaa tttatggaaa acgaaacggc
    17941 acgatacgtt aagtaattga gagaattgta gtcgtaacgg caagaaatgc tccacatttg
    18001 agaaaataat gattaccatt cccatttata acaagagcgt aacgatgatt acgcttagcg
    18061 aagcattgtg aagcagcaaa aatatcggtt catcaaaggg agtcgtcatg catttacgtc
    18121 atctgttttc atcgcgcctg cgtggttcat tactgttagg ttcattgctt gttgcttcat
    18181 cattcagtac gcaggccgca gaagaaatgc tgcgtaaagc ggtaggtaaa ggtgcctacg
    18241 aaatggctta tagccagcaa gaaaacgcgc tgtggctcgc cacttcgcaa agccgcaaac
    18301 tggataaagg cggcgtggtt tatcgtcttg atccggttac tctggaagtg acgcaggcga
    18361 tccataacga tctcaagccg tttggtgcca ccatcaataa cacgactcag acgttgtggt
    18421 ttggtaacac tgtaaacagc gcggtcacgg cgatagatgc caaaacgggc gaagtgaaag
    18481 gacgtctggt gcttgatgag cgtaaacgta ccgaagaagt gcgtccgttg cagccgcgtg
    18541 agctggtagc tgatgatgcc acgaacaccg tttacatcag tggtattggt aaagagagcg
    18601 tgatttgggt cgttgatggc gagaatatca aactgaaaac cgccatccag aacaccggta
    18661 aaatgagtac cggtttagcg ctggatagca aaggcaaacg tctttacacc actaacgctg
    18721 acggcgaatt gattaccatc gacaccgccg acaataaaat cctcagccgt aaaaagctgc
    18781 tggatgacga caaagagcac ttctttatca acatcagcct tgataccacc aatcagcgtg
    18841 catttatcac cgattctaaa gcggcagaag tgttagtggt tgatacccgt aatggcaata
    18901 ttcttgcgaa ggttgcggca ccggaatcac tggctgtgct gtttaaccca gcgcgtaatg
    18961 aagcctacgt gacgcatcgt caggcaggta aagtcagtgt gattgacgcg aaaagctata
    19021 aagtggtgaa aacgttcgat acgccgactc atccgaacag cctggcgctg tctgcagatg
    19081 gcaaaacgct gtatgtcagt gtgaaacaaa aatccactaa acagcaggaa gctacccagc
    19141 cggacgatgt gattcgtatt gcgctgtaat agatatggcg agggtgaaaa cggagagact
    19201 tgcctgactt ttatcacgcc gtatccggca ccttcgtcag taatttgaat ttgcccggca
    19261 cgtagccggg caattttgca attaactcgc cgtttcaggc ttaaacacaa tttcctgttg
    19321 ttcttcatct tcctcgacga ctggcgcagt gctgtgaatt tcagcaacgc gtttgcgcac
    19381 accaaaccag cctataacca gcagaatacc gataatcggt agcgccgcga tagtataagt
    19441 cccgttcggg taatcgaacg ccatcagcac cagaacactc agtaaaaaca gtaatgtcag
    19501 ccaggaagtg aagggcgcgc caggcagttt aaaactgaca tccgctgctt tgccttcttt
    19561 aatcgcttta cgcaggcgca tctggcacac gatgataaac gcccatgaag cgatgattcc
    19621 cagcgacgcg aagttcaaca caatctcaaa tacgcgcgac ggcaccagat agttgaggaa
    19681 tacgccgacg acatacacaa ccagtgtcgc cagaatcccg gcatacggca catgctgacg
    19741 actcattttc gccataaaac tcggtgcgga accgcccatc gccattgagc gcagaatgcg
    19801 tccggtgcag tacagacctg aattcaggct ggagagggca gcggtcagca ccacaatgtt
    19861 cataatgctg ccgatatatg gcacacccag tttagagaaa aacgtcacga acggactttg
    19921 ccccgcctga tacgcgctcc acggcaatag cataaccagc aacaccacgg agccgacgta
    19981 aaacaggcca atacgccaaa tcacactgtt aatcgcttta ggcaccatgg tctgcggatc
    20041 tttacattct ccggccgctg tacccaccat ttcaatagag gcaaaagcaa aaactacgcc
    20101 ctgaatcaac accagcgcag gcagcaaacc gtgggggaag aagccgccat tatcggtaat
    20161 taaatgaaag ccagtggtgt tgccatccag cggctgaccg ctaccgagga acactgtacc
    20221 cacgaccaaa aaggtcacga tggcgagcac tttaataagc gcaaaccaga actccatctc
    20281 cgcaaaccat ttcacaccaa tcatattcat ggtgccaacg atggtcagcg cagcgagcgc
    20341 aaagacccac tgcggcacgc cgccaaacgc accccagtaa tgcatataca gagcgacggc
    20401 ggtgatatcg acaatccctg tcatcgccca gttgatgaag tacatccagc ctgcaacgta
    20461 agcggctttc tctcctaaaa actcacgggc ataagagaca aaactgccac tggaagggcg
    20521 gtgtagcacc agctcaccca atgcacgcag aataaaaaat gaaaacaggc cgcagattaa
    20581 atagaccagt gccagtgctg gccccgccat ttgcagtcgg gctcctgcac ctaaaaacaa
    20641 gccggtgcca atcgcgccgc caatggcgat catctgaacc tggcgattgc ccatcgcttt
    20701 gtgatacccc tcttcgtggg cattaagcca gcggcgtttc gcggcgtgct gatctgaagt
    20761 gtcggtgtcg tgtttactca ttgctctccc tgattgcttt aatgaaaaag tcatataagt
    20821 tgccatgaac aatgtttatt catcctggcg atgatagtta tcaccattat tctttatggg
    20881 tataaaacta tcggcgcagc atcctacccg ttatcgataa acgatgcaaa acatcccctt
    20941 acaatcctga aggggaataa tacaactgac gaaaaaatga caaatccttt tgctggttaa
    21001 cctgtgtact gtcctacact taatctttaa aagattgtga ggggcatatg attaaggtgt
    21061 atggcgtacc cggctggggc tctacaatca gtgagctgat gctaacactg gctgatattc
    21121 cttatcagtt cgttgatgtc agtggttttg accgcgaggg aacttcacgc gagttattga
    21181 aaaccctgaa cccgttgtgt cagattccga cactggcgct ggaaaatgac gagattatga
    21241 cggagacagc ggcgattgca ttgatggttc tcgatcgtcg cccggacctt gcgccaccgc
    21301 ttgggcgcgt cgaacgtcag cagtttcaac agttattggt ctggctggtt gctaatgtct
    21361 atccaacatt cactttcgcc gattaccctg agcgttgggc tcctgacgca cccgaacagt
    21421 taaagaataa tgttattgaa tatcgtaaat cgctttatat ctggctgaat tcacaattga
    21481 ccgctgaacc gtatgtgttt ggagagcaac taacgctggt ggactgttat ctctgcacca
    21541 tgcgcacatg gggaccggga cacgaatggt ttcaggataa tgccccgaac atcaatgcga
    21601 ttgctgatgc tgtatgtcag ataccaaagc taaaagggat actaaagagg aatggaatca
    21661 tttaattatt tttcccatcg atttcgccca ctttctttcg cccggtataa cgcagcgtcg
    21721 gcatctgaaa taatttgttc agccgttttt gcgggagcag aacaggtaat cccctgacta
    21781 accgtaacga atgcattaac agcagatgcc tgatgcggga tagctgccag tttaagttct
    21841 tgcttcactc tggctgctac cgtctctgct tccggcaatg ttgcattaaa aagtatgatg
    21901 acaaactctt cgccaccata acggacaaca acatcctccg actggcgagt tgaacgctgt
    21961 agagcgtcgg caacttgtat tagacaacga tccccagcag gatgcccgta agtgtcgttg
    22021 taacgtttaa aaaaatcgac atccagcata ataagagcaa atgacccgcc gttatctatc
    22081 gcgtcgtgta agatactgtt cattgcacgt cggtttgcta tcccagttaa aggatcacga
    22141 tgtgctaatg catcaaggcg attaattaac tggttgttat aactgttttg tacccatgct
    22201 tcttcaaacc aacgtaacaa aatgagtcgc ccaccatata gcgccagtga aaaaatacac
    22261 catacaacag caaaatgaat attgatctgt tgattaagca taagactcgc tattggagtg
    22321 gtgaaccata agggaatgac aaatgcgagc aatgatgggg tatggaaata aagtgcgata
    22381 acgccactaa gcattaataa tacacatagt ggataagcaa actgcagcgt ccagtacgca
    22441 ataaagtgat aactacacca tgaccatagc aggctcaggg caactaatag cacctggata
    22501 ggtaatgttt tttgccgagg gatgagtagt aaaccactgc aaatcagcag aatacccgtt
    22561 gcagatatat caatagaatg tataaggctt ttttcattta accagggtgg agtaaggtca
    22621 ttaaacagaa cacggcgcag caaaattatc aatgcaaaac tgacattgat aaaagcaaac
    22681 catggcaaac taatcgtcat gccgcgaata accatatcgc ggcgggtttt ccatgagtga
    22741 tgcgtttttc ttcgttcagc cattttttaa ccaaaaaagg gcaggatacc tgcccggtat
    22801 gcagtaaatt tatggtgtca ctatattgaa ccaaaattca aaattatcca tataacctaa
    22861 cagctcgttg agtttttccg cattgccagt aattttgaca tcttctttgg ccgtggcttc
    22921 tttcagcgtc tcttctttca gtacaatttt gttcaataca tcacgagaca gagtgatagt
    22981 ggcatcagca tccgaagcct ctacaccagc ggtatgattg agaacaccat tttcaagctc
    23041 cactttatag gtgccgccat cttcaccaaa atcgaaattc aacacggttt tggcatctgc
    23101 cgctttctca ccattaatat ggacggcaag ataatcaaag aacatctccg gcgtcatcgc
    23161 acgcacggtg tctgggctgg cagtatttgg tgttggtaat ttttgtacgc cattacgcaa
    23221 ttcctgtgcg ccagtgagat agaaattacg ccacggccca gattcagcct gatagcctaa
    23281 ctgctcaagc gcatcagctt ccagatttct cgctgcctgg ttgtttggat cggtaaacac
    23341 caccttacta acaacttggg caacccagcg gaaattaccc tggtcataat cctgtttggc
    23401 tttctgtaaa atggcatcag caccgcccat atactcgacg aatttttttg cgccttcttc
    23461 tggcggcagt tcatcaagcg tcgcggggtt accatcaaac cagccgagat aaagcacgta
    23521 agttgctttc acgtcatgac ttaccgaacc gtaatagccg cgattcgccc aggtattagc
    23581 cagtgaagag ggcagtttaa agtttgcggc aatttcatca cgggtcatcc cttgatttgc
    23641 catacgcaga gtttgatcat tgatgtaacg atacaaatca cgctggcttt taagcagttt
    23701 gaccacgtta tcgttgcccc aggttggcca gtgatgttga gcgagaataa tctcggcttt
    23761 atctccccat aaattgagag cttcgttaat atattttgac cacggcagcg gttggcgaat
    23821 tttagcccca cgcagcgaat aggtgttatg aagggtgtgc gtgacatctt cagcggtttc
    23881 gatcagtttc ttttcttcaa taaaccacag catttcagaa ggcgcttcag agcctggcgc
    23941 catcaagaaa tcataggtca acccgtcaat agtttctttt tgtccggttt tggtgatgta
    24001 gttagtagga gcaatcaacg tgaccgttcc tgctgaagtg gttgttccca gtcccgcgcc
    24061 aacctggcct ttaacatcag gtttcaataa gttgccatac atataactgg ctcgtcggct
    24121 catcacattg ccagccataa tattttcgga aacggcttcc tccaaaaatc cggctggcgc
    24181 gtagatttta actttgcctg atttaacgtc agcttcatca attacgccgc gaacaccacc
    24241 gtagtgatcg acatgactgt gtgtgtagat aaccgctact accggttttt tgtcgcgatt
    24301 tttgtaatag agatccatac ccactttggc tgtttctgct gaaacaaggg gatcaacgat
    24361 ggtaataccc tctttgcctt caatgatggt catatttgat agatcgagat tgcgaatctg
    24421 atagacacct tcagttactt cgaataaccc gctgatattt atcagttgcg cctgacgcca
    24481 taaacttgga ttcaccgtat caggggcttt atcaccttct ttaataaaag cgtattgttg
    24541 tggatcccag ataacatttc ctttttcgcc cttaatcacg tcttgaggga taggggcaac
    24601 aaaaccttta tgggcatcag taaagtcagt gttatcagaa aacggcaact ggttgtagag
    24661 taaattgttt gcttgttgcg tggctgcggt ggcgtcttta ggggcttccg ccgcgaggag
    24721 tggcaaagaa ctggtggtga ataatcccgt tattagcaga cttttaacaa tatgattaag
    24781 ctgcatctta agcgtctcca gaatggactt ttattggggg taattacgga tgaaaatttt
    24841 tgtgagggca agaataacta taatgggcgt taataaattg gcaagccggg aacttgatgt
    24901 tttcttaatg gcatgttggc gaagaatata ttctgcaagt tgacgttttt gttggcgctt
    24961 tatttaaaag ggtgtagtac aaatctgtac aaattcaagt aggcattatt gccgtaaaaa
    25021 agcatcaccg gacacataac attcacaacg tgtgttaatt ttactgacat ctttcaaaag
    25081 gagcacaatc atgccgcaca tagacattaa atgttttcca cgtgaactgg acgaacaaca
    25141 aaaagcagca cttgctgcag atattaccga cgttattatt cgccatctga acagtaaaga
    25201 cagttcgata agcattgctc tacagcagat tcaaccagaa tcatggcaag ctatctggga
    25261 caccgaaatt gcgccccaaa tggagacgct gataaagaaa cctggttaca gcatgtaatg
    25321 cttaacctgc cgtaacacgc ttgccgctgg taacaaacgt gccagcgcct aaatgatgca
    25381 acgtattgag cttatcatca tcaaactgcc agcggccttc gacaaatacc cgtacgtctg
    25441 ccgctgcaga taccacttcg ccaaatagcg aatcgtattt ttcttgcgcg gaagttgcag
    25501 gtagcaggcg acactccatc cacgc
//
LOCUS       DAMZFT010000068        25470 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.068, whole genome shotgun sequence.
ACCESSION   DAMZFT010000068 DAMZFT010000000
VERSION     DAMZFT010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 25470)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25470)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25470
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.068"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            355..1179
                     /gene="gadX"
                     /locus_tag="PN977_002857"
     CDS             355..1179
                     /gene="gadX"
                     /locus_tag="PN977_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadX"
                     /protein_id="HDI6042875.1"
                     /translation="MQPLHGNCLIAYARHKYILTMVNGEYRYFNGGDLVFADASQIQV
                     DKCVENFVLVSRDTLSLFLPMLKEEALKLHAHKKVPSLLVHHCTRDIPVFQEVAQLSQ
                     NKNLRYAEMLRKRALIFALLSVFLEDTQFIPLLLNVLQPNMRTRVCTVINNNIAHEWT
                     LARIASELLMSPSLLKKKLREEGTSYSQLLTECRMQRALQLIVIYGVSIKRVAVSCGY
                     HSVSYFIYVFRNYYGMTPTEYQERSAQELPNCGPAASIAAQGNFYGTDRSAEGIRL"
     gene            1548..2276
                     /gene="gadW"
                     /locus_tag="PN977_002858"
     CDS             1548..2276
                     /gene="gadW"
                     /locus_tag="PN977_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadW"
                     /protein_id="HDI6042876.1"
                     /translation="MTHVCSVILVRRSFDIYHEQQKISLHNESILLLDKNLADDFAFC
                     SPDTRRLDIDELTVCHYLQNIRQIPRNLGLHSKDRLLINQSPPMPLVTAIFDSFNDPR
                     VNSPILSKMLYLSCLSMFSHKKELIPLLFNSISTVSGKVERLISFDIAKRWYLRDIAE
                     RMYTSESLIKKKLQDENTCFSKILLASRMSMARRLLELRQIPLHTIAEKCGYSSTSYF
                     INTFRQYYGVTPHQFAQHSPGTFS"
     gene            complement(2639..5752)
                     /locus_tag="PN977_002859"
     CDS             complement(2639..5752)
                     /locus_tag="PN977_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit"
                     /protein_id="HDI6042877.1"
                     /translation="MANYFIDRPVFAWVLAIIMMLAGGLAIMNLPVAQYPQIAPPTIT
                     ISATYPGADAQTVEDSVTQVIEQNMNGLDGLMYMSSTSDAAGNASITLTFETGTSPDI
                     AQVQVQNKLQLAMPSLPEAVQQQGISVDKSSSNILMVAAFISDNGSLNQYDIADYVAS
                     NIKDPLSRTAGVGSVQLFGSEYAMRIWLDPQKLNKYNLVPSDVISQIKVQNNQISGGQ
                     LGGMPQAADQQLNASIIVQTRLQTPEEFGKILLKVQQDGSQVLLRDVARVELGAEDYS
                     TVARYNGKPAAGIAIKLATGANALDTSRAVKEELNRLSAYFPASLKTVYPYDTTPFIK
                     ISIQEVFKTLVEAIILVFLVMYLFLQNFRATIIPTIAVPVVILGTFAILSAVGFTINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVIAEDKLPPKEATHKSMRQIQRALVGIAVVLS
                     AVFMPMAFMSGATGEIYRQFSITLISSMLLSVFVAMSLTPALCATILKAAPEGGHKPN
                     ALFARFNTLFEKSTQHYTDSTRSLLRCTGRYMVVYLLICAGMAVLFLRTPTSFLPEED
                     QGVFMTTAQLPSGATMVNTTKVLQQVTDYYLTKEKDNVQSVFTVGGFGFSGQGQNNGL
                     AFISLKPWSERVGEENSVTAIIQRAMIALSSINKAVVFPFNLPAVAELGTASGFDMEL
                     LDNGNLGHEKLTQARNELLSLAAQSPNQVTGVRPNGLEDTPMFKVNVNAAKAEAMGVA
                     LSDINQTISTAFGSSYVNDFLNQGRVKKVYVQAGTPFRMLPDNINQWYVRNASGTMAP
                     LSAYSSTEWTYGSPRLERYNGIPSMEILGEAAAGKSTGDAMKFMADLVAKLPAGVGYS
                     WTGLSYQEALSSNQAPALYAISLVVVFLALAALYESWSIPFSVMLVVPLGVVGALLAT
                     DLRGLSNDVYFQVGLLTTIGLSAKNAILIVEFAVEMMQKEGKTPIEAIIEAARMRLRP
                     ILMTSLAFILGVLPLVISHGAGSGAQNAVGTGVMGGMFAATVLAIYFVPVFFVVVEHL
                     FARFKKA"
     gene            complement(5777..6934)
                     /gene="mdtE"
                     /locus_tag="PN977_002860"
     CDS             complement(5777..6934)
                     /gene="mdtE"
                     /locus_tag="PN977_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter subunit MdtE"
                     /protein_id="HDI6042878.1"
                     /translation="MNRRRKLLIPLLFCGAMLTACDDKSAENAAAMTPEVGVVTLSPG
                     SVNVLSELPGRTVPYEVAEIRPQVGGIIIKRNFIEGDKVNQGDSLYQIDPAPLQAELN
                     SAKGSLAKALSTASNARITFNRQASLLKTNYVSRQDYDTARTQLNEAEANVTVAKAAV
                     EQATINLQYANVTSPITGVSGKSSVTVGALVTANQADSLVTVQRLDPIYVDLTQSVQD
                     FLRMKEEVASGQIKQVQGSTPVQLNLENGKRYSQTGTLKFSDPTVDETTGSVTLRAIF
                     PNPNGDLLPGMYVTALVDEGSRQNVLLVPQEGVTHNAQGKATALILDKDDVVQLREIE
                     ASKAIGDQWVVTSGLQAGDRVIVSGLQRIRPGIKARAISSSQENASTESKQ"
     gene            6994..7323
                     /locus_tag="PN977_002861"
     CDS             6994..7323
                     /locus_tag="PN977_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001205317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6042879.1"
                     /translation="MRDLQTSGIVGLSASKMGCKHNDRHGEVNDKKNVAVLPTVSASI
                     RATKGSTGGYTYQGNKVVFVSPSIITGGIEISICIPRHASHQFQLLMSLLKIRCDTQG
                     DDVAHTS"
     gene            complement(7273..7800)
                     /gene="gadE"
                     /locus_tag="PN977_002862"
     CDS             complement(7273..7800)
                     /gene="gadE"
                     /locus_tag="PN977_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadE"
                     /protein_id="HDI6042880.1"
                     /translation="MIFLMTKDSFLLQGFWQLKDNHEMIKINSLSEIKKVGNKPFKVI
                     IDTYHNHILDEEAIKFLEKLDAERIIVLAPYHISKLKAKAPIYFVSRKESIKNLLEIT
                     YGKHLPHKNSQLCFSHNQFKIMQLILKNKNESNITSTLNISQQTLKIQKFNIMYKLKL
                     RRMSDIVTLGITSYF"
     gene            complement(8598..9170)
                     /gene="hdeD"
                     /locus_tag="PN977_002863"
     CDS             complement(8598..9170)
                     /gene="hdeD"
                     /locus_tag="PN977_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-resistance protein HdeD"
                     /protein_id="HDI6042881.1"
                     /translation="MLYIDKATILKFDLEMLKKHRRAIQFIAVLLFIVGLLCISFPFV
                     SGDILSTVVGALLICSGIALIVGLFSNRSHNFWPVLSGFLVAVAYLLIGYFFIRAPEL
                     GIFAIAAFIAGLFCVAGVIRLMSWYRQRSMKGSWLQLVIGVLDIVIAWIFLGATPMVS
                     VTLVSTLVGIELIFSAASLFSFASLFVKKQ"
     gene            9425..9757
                     /gene="hdeA"
                     /locus_tag="PN977_002864"
     CDS             9425..9757
                     /gene="hdeA"
                     /locus_tag="PN977_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeA"
                     /protein_id="HDI6042882.1"
                     /translation="MKKVLGVILGGLLLLPVVSSAADAQKAADNKKPVNSWTCEDFLA
                     VDESFQPTAVGFAEALNNKDKPEDAVLDVQGIATVTPAIVQACTQDKKASFKDKVKGE
                     WDKIKKDM"
     gene            9873..10199
                     /gene="hdeB"
                     /locus_tag="PN977_002865"
     CDS             9873..10199
                     /gene="hdeB"
                     /locus_tag="PN977_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeB"
                     /protein_id="HDI6042883.1"
                     /translation="MNIASLRKAFIFMGAVAALSLVNAQSALAANESAKDMTCQEFID
                     LNPKAMTPVAWWMLHEETVYKGGDTVTLNETDLTQIPKVIEYCKKNPQKNLYTFKNQA
                     SNDLPN"
     gene            10263..10910
                     /locus_tag="PN977_002866"
     CDS             10263..10910
                     /locus_tag="PN977_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HDI6042884.1"
                     /translation="MTAEFIIRLILAAIACGAIGMERQMRGKGAGLRTHVLIGMGSAL
                     FMIVSKYGFADVLSLDHVGLDPSRIAAQVVTGVGFIGAGNILVRNQNIVGLTTAADIW
                     VTAAIGMVIGSGMYELGIYGSVMTLLVLEVFHQLTFRLMNKNYHLQLTLVNGNTVSML
                     DWFKQQKIKTDLVSLQENEDHEVVAIDIQLHATTSIEDLLRLLKGMAGVKGVSIS"
     gene            complement(10962..11732)
                     /locus_tag="PN977_002867"
     CDS             complement(10962..11732)
                     /locus_tag="PN977_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter ATP-binding protein"
                     /protein_id="HDI6042885.1"
                     /translation="MISAQNLVYSLQGRRLTDNVSLTFPGGEIVAILGPNGAGKSTLL
                     RQLTGYLQPDSGECRLFNKPLNEWSITELAKHRAVMRQNSHMAFPFSVQEVIQMGRHP
                     HRTGNQDNETAQIIALCDCQALANRDYRQLSGGEQQRVQLARLLVQLWEPTPSPKWLF
                     LDEPTSALDIHHQQHLFRLLRQLVHERQFNVCCVLHDLNLAARYADRVVLMQKGKVIA
                     NGKPQDVLTQQALTMLYGADITVLKDPANHSPLIVLDH"
     gene            complement(11729..12685)
                     /gene="chuU"
                     /locus_tag="PN977_002868"
     CDS             complement(11729..12685)
                     /gene="chuU"
                     /locus_tag="PN977_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter permease ChuU"
                     /protein_id="HDI6042886.1"
                     /translation="MGLSLLLLALVLFGASQGALKISFDALFDEEYRDIWLNIRLPRV
                     LLAVLVGAALATAGVIMQGLFRNPMADPGLLGVSSGSALMVGVAIVLPFSFPVVLVLY
                     EQMVFAIAGSLVVCTIIFLITQRHRDGSMMQLLLAGIAINALCGAAIGILSYIGDEQQ
                     LRQLTLWMMGNLGQAQWPTLLVASSFILPAIIATTCLAGTLNLLQLGDEEAHYLGVNV
                     KRKRQQLLLVSSLLVGAAVSVSGIIGFIGLVIPHLIRMTTGANHRWLIPCSALAGACL
                     LLMADTLARTLVQPAEMPVGLLTSLLGGPYFMWLILRNRRIT"
     gene            complement(12770..13393)
                     /gene="chuY"
                     /locus_tag="PN977_002869"
     CDS             complement(12770..13393)
                     /gene="chuY"
                     /locus_tag="PN977_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobilin reductase"
                     /protein_id="HDI6042887.1"
                     /translation="MTPWLLFGAGGKGVGARTLELALAEQRPVVAVIRHADAATKLAQ
                     QGVQVFTGDACDASVVAAACRAAGPDALIISTMGGAQDYLAHRTVIDEAEKAGISRMI
                     LVTSLGCGDSWPFLSERAKAAFGQAVREKTLAESWLQTSQLDYAILRPGGLLDGAATG
                     KAQRIQNQECHGFVHRADVAAHIHELANAPALNQQVYSLIEPDLKPA"
     gene            complement(13393..13887)
                     /gene="hutX"
                     /locus_tag="PN977_002870"
     CDS             complement(13393..13887)
                     /gene="hutX"
                     /locus_tag="PN977_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme utilization cystosolic carrier protein
                     HutX"
                     /protein_id="HDI6042888.1"
                     /translation="MSHVSLQEFLKTEPDGTLEVVAEQYNTTLLEVVKNLPSSTIVSG
                     DKFDTVWDTVCEWGNVTTLVHTADVILEFSGELPSGFHRHGYFNLRGKHGMSGHIKAE
                     NCTHIALIERKFMGMDTASILFFNKEGSAMLKIFLGRDDHRQLLSEQVSAFHALEASL
                     KEHA"
     gene            complement(13900..15237)
                     /locus_tag="PN977_002871"
     CDS             complement(13900..15237)
                     /locus_tag="PN977_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme anaerobic degradation radical SAM
                     methyltransferase ChuW/HutW"
                     /protein_id="HDI6042889.1"
                     /translation="MNTNNTLDLTPHFALDGDQPFKDRRAMMPFRGAIPVAKEQLAQT
                     WQEMINQTVSPRKRLVYLHIPFCATHCTFCGFYQNRFNEDACAHYTDALIREIELEAD
                     SVLHQSAPIHAVYFGGGTPSALSAHDLARIITTLREKLPLAPDCEITIEGRVLNFDAE
                     RIDACLDAGANRFSIGIQSFNSKIRKKMARTSDGPTAIAFMESLVKRDRAAVVCDLLF
                     GLPGQDAQTWGEDLAIARDIGLDGVDLYALNVLPNTPLGKAVENGRTTVPSPAERRDL
                     YLQGCDFMDDAGWRCISNSHWGRTTRERNLYNLLIKQGADCLAFGSGAGGSINGYSWM
                     NERNLQTWHESVAAGKKPLMMIMRNAERDAQWRHTLQSGVETARVPLDELTPHAEKLA
                     PLLAQWHQKGLSRDASTCLRLTNEGRFWASNILQSLNELIQVLNAPAIVREKP"
     gene            complement(15257..16171)
                     /gene="chuT"
                     /locus_tag="PN977_002872"
     CDS             complement(15257..16171)
                     /gene="chuT"
                     /locus_tag="PN977_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312409.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter substrate-binding protein
                     ChuT"
                     /protein_id="HDI6042890.1"
                     /translation="MPRIITRPFLFSPLTLCISAVASAAKTMVKRKKLFTALLALSWT
                     FSVTAAERIVVAGGSLTELIYAMGAGKRVVGVDETTSYPPETARLPHIGYWKQLSSEG
                     ILSLRPDSVITWQDAGPQIVLDQLRAQKVNVVTLPRVPATLEQMYANIRQLAKTLQVP
                     EQGEALVTQINQRLERVQQNVAAKKAPVKAMFILSAGGSAPQVAGKGSVADAILSLAG
                     AENVATHQQYKSYSAESLIAANPEVIVVTSQMVDGDINRLRSIAGITHTAAWKNQRII
                     TVDQNLILGMGPRIADVVESLHQQLWPQ"
     gene            16855..18837
                     /gene="chuA"
                     /locus_tag="PN977_002873"
                     /gene_synonym="shuA"
     CDS             16855..18837
                     /gene="chuA"
                     /locus_tag="PN977_002873"
                     /gene_synonym="shuA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent heme/hemoglobin receptor
                     ChuA/ShuA"
                     /protein_id="HDI6042891.1"
                     /translation="MSRPQFTSLRLSLLALAVSATLPTFAFATETMTVTATGNARSSF
                     EAPMMVSVIDTSAPENQTATSATDLLRHVPGITLDGTGRTNGQDVNMRGYDHRGVLVL
                     VDGVRQGTDTGHLNGTFLDPALIKRVEIVRGPSALLYGSGALGGVISYDTVDAKDLLQ
                     EGQSSGFRVFGTGGTGDHSLGLGASAFGRTENLDGIVAWSSRDRGDLRQSNGETAPND
                     ESINNMLAKGTWQIDSAQSLSGLVRYYNNDAREPKNPQTVEASDSSNPMVDRSTIQRD
                     AQLSYKLAPQGNDWLNADAKIYWSEVRINAQNTGSSGEYREQITKGARLENRSTLFAD
                     SFASHLLTYGGEYYRQEQHPGGATTGFPQAKIDFSSGWLQDEITLRDLPITLLGGTRY
                     DSYRGSSDGYKDVDADKWSSRAGMTINPTNWLMLFGSYAQAFRAPTMGEMYNDSKHFS
                     IGRFYTNYWVPNPNLRPETNETQEYGFGLRFDDLMLSNDALEFKASYFDTKAKDYIST
                     TVDFAAATTMSYNVPNAKIWGWDVMTKYTTDLFSLDVAYNRTRGKDTDTGEYISSINP
                     DTVTSTLNIPIAHSGFSVGWVGTFADRSTHISSSYSKQPGYGVNDFYVSYQGQQALKG
                     MTTTLVLGNAFDKEYWSPQGIPQDGRNGKIFVSYQW"
     gene            18886..19914
                     /gene="chuS"
                     /locus_tag="PN977_002874"
     CDS             18886..19914
                     /gene="chuS"
                     /locus_tag="PN977_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hematinate-forming heme oxygenase ChuS"
                     /protein_id="HDI6042892.1"
                     /translation="MNHYTRWLELKEQNPGKYARDIAGLMNISEAELAFARVTHDAWR
                     MRGDIRDILAALESVGETKCICRNEYAVHEQVGAFTNQHLNGHAGLILNPRALDLRLF
                     LNQWASVFHIKENTARGERQSIQFFDHQGDALLKVYATDNTDMAAWSELLARFITDEN
                     TPLELKAVDAPVVQTRADASVVEQEWRAMTDVHQFFTLLKRHNLTRQQAFNLVADDLA
                     CKVSNSALAQILESAQQDGNEIMVFVGNRGCVQIFTGVVEKVVPMKGWLNIFNPTFTL
                     HLLEESIAETWVTRKPASDGYVTSLELFAHDGTQIAQLYGQRTEGEQEQAQWRKQIAS
                     LIPEGVTA"
     gene            complement(19975..20505)
                     /locus_tag="PN977_002875"
     CDS             complement(19975..20505)
                     /locus_tag="PN977_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDI6042893.1"
                     /translation="MFLIITRDTMFFTAMKNILSKGNVVHIQNEEEIDVMLHQHAFVI
                     IDTLMNNVFHSNLLTQIERLKPVHVIIFSPFNIKRCLGKVPVTFVPRTITIIDFVALI
                     NGSYCSVPEADVALSRKQHQVLSCIANQMTTEDILEKLKISLKTFYCHKHNIMMILNL
                     KRINELVRHQHIDYLV"
     gene            complement(20658..21224)
                     /gene="slp"
                     /locus_tag="PN977_002876"
     CDS             complement(20658..21224)
                     /gene="slp"
                     /locus_tag="PN977_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312404.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein Slp"
                     /protein_id="HDI6042894.1"
                     /translation="MNMTKGALILSLSFLLAACSSIPQNIKGNNQPDIQKSFVAVHNQ
                     PGLYVGQQARFGGKVINVINGKTDTLLEIAVLPLDSYAKPDIEANYQGRLLARQSGFL
                     DPVNYRNHFVTILGTIQGEQPGFINKVPYNFLEVNMQGIQVWHLREVVNTTYNLWDYG
                     YGAFWPEPGWGAPYYTNAVSQVTPELVK"
     gene            complement(21470..22693)
                     /locus_tag="PN977_002877"
     CDS             complement(21470..22693)
                     /locus_tag="PN977_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6042895.1"
                     /translation="MSIDFTPGMINTYHGDIYNRTTDTDNVKTPDTPMWPCDNQEEQQ
                     PINFSLSEEGYNAEQFDLAQQQLQQINACHTNTTYTNADYSKVVAQLVKLINNIETIS
                     STPLTQQTQSILNQINNIRYEKNKNSESKIIVVFNPKPNKPIITKILVEEGIPVRFSV
                     QTVSSENKNFSGLRTDLPTDIKDIQRLYQKMTRLYIEHSENENRMKVFAGTNFIDFNM
                     TGQDLSGFVLTLSRFYFEDLLNVNFTDANLFDTIFSHKENPITKLHKDEQYVDKQING
                     LFSTLLTINDNSLRAKAEIASTIIELLETRVNNLSYNDIIKYQQEFQKECYKQVKDFT
                     TPSRYNRIKKWAQMSEYQFEIFQHDTFHPKKMSYAPYLKRSLPDEKTINYGVEIETPI
                     GKRIRLSNHYQNIIP"
     gene            complement(23273..24274)
                     /locus_tag="PN977_002878"
     CDS             complement(23273..24274)
                     /locus_tag="PN977_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000100290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HDI6042896.1"
                     /translation="MSSWLAMLQDAAEMFVFLAVELSLLFIVISAGVSLIRQKVPDHK
                     IQQMMGARKGRGYLLAALLGAVTPFCSCSTIPMLRGLLSAKAGFGPTLTFLFVSPLLN
                     PIIVGLMWVTFGWKVTLLYAIIAAGVSVLASIILDSLGFERHIIASKSSSANCCAPAK
                     TSPGTTYTPIEVSCCSPTAKAIEKPVVNCCNTKAVVSINPIKLATKDALQQFKDVLPY
                     LLLGVLIGSFIYGFIPSEWIAAHAGADNPFAIPLSAVVGIPLYIRAEAVIPLASVLMT
                     KGMGLGALMALIIGSAGASLTEVILLKSMFRIPMIVAFLTVILGMAILMGYLTQMLF"
     gene            24381..24701
                     /locus_tag="PN977_002879"
     CDS             24381..24701
                     /locus_tag="PN977_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001614035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDI6042897.1"
                     /translation="MQLEEVAKALKELGHPTRLFIFKHLVKAGEQGLPVGELQKQLGI
                     PGSTLSHHISALVSVGLVKQNRESRTLMCVSQYAMLEAIIEFLREECCVNSKTDGEGA
                     TKNK"
     gene            complement(24722..25147)
                     /gene="arsC"
                     /locus_tag="PN977_002880"
     CDS             complement(24722..25147)
                     /gene="arsC"
                     /locus_tag="PN977_002880"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-dependent arsenate reductase"
                     /protein_id="HDI6042898.1"
                     /translation="MSNITIYHNPACGTSRNTLEMIRNSGTEPTIIYYLETPPTRDEL
                     IKLIADMGISVRALLRKNVEPYEELGLAEDKFTDDRLIDFMLQYPILINRPIVVTPLG
                     TRLCRPSEVVLEILPDAQQRAFTKEDGEKVVDEAGKRLK"
     gene            complement(25160..>25470)
                     /locus_tag="PN977_002881"
     CDS             complement(25160..>25470)
                     /locus_tag="PN977_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="arsenical efflux pump membrane protein ArsB"
                     /protein_id="HDI6042899.1"
                     /translation="TAFLSSIMNNMPTVLVGALSIDGSTASGVIKEAMVYANVIGCDL
                     GPKITPIGSLATLLWLHVLSQKNMTISWGYYFRTGIIMTLPVLFVTLAALALRLSFTL
                     "
BASE COUNT         6831 a         5761 c         5982 g         6896 t
ORIGIN      
        1 taacgttgta aaaaccgaat gcccaacctt taaaaaacag ttgggcattc ggttgcttat
       61 taatcgcaat aatatattgg ctgtctattc atcgtgttga taagatatat gttatatgaa
      121 tgtttattca atgtaacgga tattcttaat atcactacaa gagattattt gcttactatt
      181 aatttcactg cgttcttaaa cacgcctttt gtattataaa tccagtcatc ctgcccgtag
      241 attatcatcc tgttttcccg cgtcgtttaa atttatttat caatcaattt gacttaagag
      301 ggcggcgtgc tacattaata aacagtaata tgtttatgta atattaagtc aactatgcaa
      361 ccactacatg gtaattgtct aattgcgtac gcaagacata aatatattct caccatggtt
      421 aatggtgaat atcgctattt taatggcggt gatctggttt ttgcggatgc aagccaaatt
      481 caagtggata agtgtgttga gaatttcgta ttagtttcaa gagacacgct ttcattattt
      541 ctcccgatgc tcaaggagga ggcattaaaa cttcatgcac ataaaaaagt tccttcatta
      601 ctcgttcatc actgtaccag agatattcct gtttttcagg aagttgcgca actatcgcag
      661 aataagaatc ttcgctatgc agaaatgcta cgtaaaaggg cattaatctt tgcgttgtta
      721 tctgtttttc ttgaggatac gcagtttata ccgttacttc tgaacgtgtt gcaaccgaat
      781 atgcgaacac gagtttgtac ggtcatcaat aataatatcg cccatgagtg gacattagcc
      841 cgaattgcta gcgaattgtt gatgagtcca agcctgttaa agaaaaaatt gcgcgaagaa
      901 ggtacgtcat attcacagtt gcttactgag tgtaggatgc agcgtgcttt acaacttatt
      961 gttatatatg gggtttcaat taagcgagtt gcagtatcct gtggatatca cagcgtgtcg
     1021 tatttcattt acgtttttcg aaattattat gggatgacgc ccacagagta tcaggagcga
     1081 tcggcgcagg aattaccgaa ctgtggcccg gcggcaagta tagctgcgca agggaatttt
     1141 tacggcactg accgttctgc ggaaggaata agattataga gttttactca gacataaaaa
     1201 aaacccggca taggggaccg ggaagaggat agtctgccgt ctccagacta ataaaccgtt
     1261 ataacactcc ctgttggcac gggaaacttt gtgctctcag taagttaaat ataactttta
     1321 ctggaaataa gatcagccat ttttttataa acataagcta tacgctgtgc gaaaatatat
     1381 tcgtgttgca tttacttatt gtcaattaac tgttatgcaa aactactttg tggataaatt
     1441 ttggtcctac caaatctggc agtttttgcg ctaagaaaca gtctggcatc atttcattag
     1501 tatactgaaa ttgaaataat cgcagtaatg aaatataagg gatagtcatg actcatgtct
     1561 gctcggtgat cctcgttcgt cgttcattcg atatttatca tgaacagcaa aaaatatcgt
     1621 tgcataacga gagtatcctg ctgctggata aaaatttggc agacgatttt gcgttttgtt
     1681 caccggatac gcgacgactg gatatcgatg agctgacagt ttgccattac ttacaaaata
     1741 ttcgtcagat accacgcaat ttagggttac atagcaaaga ccgtctgtta attaaccagt
     1801 caccccccat gccgctggtg acggcgattt ttgatagttt caatgacccc cgggtcaatt
     1861 cgccgatact gagcaaaatg ctctaccttt cctgtttatc aatgttttct cataagaaag
     1921 aactgatccc cttacttttc aatagtatca gtactgtttc aggaaaagtt gaacgcctta
     1981 ttagctttga tatcgccaaa cgttggtatc tgcgcgatat cgcagaaaga atgtatacca
     2041 gcgagagtct catcaaaaaa aagttgcagg atgaaaatac ctgtttcagt aaaatattac
     2101 tcgcctccag gatgtcgatg gccagacgat tactcgagtt acgtcaaatt cctctgcata
     2161 ctattgcgga aaaatgtgga tatagcagta cgtcgtactt tataaacaca tttcgacaat
     2221 attatggtgt aacgccacat cagtttgcgc aacattcgcc aggtaccttt tcctgacata
     2281 ttttgcattt gaatattggt caggatctca cacctgcttc atgtgaaact cttccctgat
     2341 gatttctgcc gggctaccgg ctaattctct ttcgcagttt gccaggcggt ataggtattt
     2401 tcaggaggaa aagactcggc atgtttggga ttattaagct gacaattcat acgattacgg
     2461 gaatgtgggt cagtattgtg ctgttcaaat tgatgacgaa tggctggagt ggtttctact
     2521 ttcagtgctg tgtactaagc ctggtattcc tgacggttag ctggttgttg agtggtgaat
     2581 ggttagcagg aaagagtaag gctgaacctt cacgttcaac cttactctca tttacacgtt
     2641 acgctttttt aaagcgggca aagagatgtt ccactacaac gaaaaagacc ggaacgaagt
     2701 aaattgccag cactgttgcg gcaaacatcc cgcccatcac gccggtacct accgcgtttt
     2761 gcgcgccaga accggcacca tgactgataa ccagcggcag cacgccgaga ataaaggcca
     2821 gagaggtcat caggattggg cgtaaacgca ttcgcgccgc ttcgatgatc gcctctatcg
     2881 gcgttttccc ttctttctgc atcatctcaa cggcaaattc gacaatcagg atggcgtttt
     2941 tggcggaaag cccgatggtg gtcagcaaac caacctggaa gtagacgtca ttacttaagc
     3001 cgcgtagatc ggtggccagt aatgcgccaa cgacgcctaa cggtacaacc aacatcaccg
     3061 agaacggaat tgaccagctc tcataaagtg cggcgagggc gaggaacacc acgaccagtg
     3121 aaatggcata cagcgcggga gcctgatttg aggataacgc ttcctgatac gacagtccgg
     3181 tccatgagta gccgacgcct gccggaagtt tagcgaccag gtctgccata aatttcatgg
     3241 cgtcaccggt acttttaccg gccgccgctt cacctaaaat ctccattgac gggatgccgt
     3301 tgtagcgttc cagtcgcggt gaaccatagg tccattcggt agacgagtag gcagaaagcg
     3361 gtgccatcgt gccagaggcg ttgcgtacat accattggtt gatgttatcc ggcaacatac
     3421 ggaacggcgt gcctgcctgg acatacactt ttttcacccg cccctggttg aggaagtcgt
     3481 tcacgtagct gctgccgaag gcggtggaaa ttgtctggtt gatatcagac agcgccacgc
     3541 ccatagcctc ggctttcgca gcgttgacgt tcactttgaa catcggcgta tcttccaggc
     3601 cgttcgggcg aaccccggtg acctgattcg gtgattgcgc tgccagtgat aacagctcgt
     3661 ttcgcgcctg ggtcagtttt tcgtgcccca ggttgccgtt gtccagcagt tccatatcaa
     3721 aacctgacgc ggtacccagt tcagccaccg cgggtaagtt gaacgggaag acgacggctt
     3781 tattgatact gcttaacgca atcattgccc gctgaatgat cgcggtaacc gagttttcct
     3841 caccgacacg ttcagaccac ggtttgaggc tgataaacgc cagaccgttg ttttgcccct
     3901 ggccgctgaa gccaaagccg ccaacggtaa acaccgactg aacattatct ttctctttag
     3961 tcagataata atccgtcacc tgttgcagca ctttcgtggt gttaaccatg gtggcaccgg
     4021 aaggtaactg agcggtggtc ataaataccc cctgatcctc ttctggtaag aaagaggtcg
     4081 gcgtacgcag gaacagcacc gccatcccgg cgcaaatcag caggtagacc accatgtagc
     4141 gaccggtaca acgcaacagc gaacgggtgc tgtcggtata gtgctgagtt gatttttcaa
     4201 acagcgtgtt gaagcgtgcg aacagggcgt taggtttgtg accgccttcc ggcgcggctt
     4261 tcagaatggt ggcgcacagg gcaggggtca ggctcattgc cacaaatact gaaagcagca
     4321 tggaggagat cagcgtgatg gagaactgtc ggtagatctc cccggttgca ccgctcataa
     4381 aggccatcgg cataaacact gcggaaagaa caacggcaat cccgaccagc gcacgttgga
     4441 tctgcctcat cgatttatgc gtcgcttcct tcggcggtag cttatcttcc gcaatgacac
     4501 gctcgacgtt ctccaccacc acgatggcgt catccaccag taaccctatc gccagcacca
     4561 tcccgaacat agttaacgtg ttgatggtga aaccgaccgc cgacaagatc gcaaacgtcc
     4621 cgagaataac caccggtacg gcaatcgtcg ggatgattgt ggcgcggaaa ttctgcaaaa
     4681 acagatacat gaccaggaag acgaggatga tagcctcaac cagcgttttg aaaacttcct
     4741 gaatagaaat tttgataaac ggtgtggtgt cgtaaggata aaccgtcttc aggcttgcag
     4801 ggaaataagc cgataaacgg ttcagctcct ctttcaccgc tcgcgaggta tccagggcgt
     4861 ttgctccggt agccagtttg atggcgatcc cggcagcagg tttgccgtta tagcgcgcca
     4921 cggtggaata atcttccgcc ccaagttcga cgcgggcgac atcacgcagc agcacttgcg
     4981 aaccatcttg ctgaactttc aacaggattt tgccaaattc ttccggtgtt tgcagacgcg
     5041 tctgcacaat gatcgaggcg ttcagttgct ggtctgccgc ctgtggcatg ccacccagtt
     5101 gaccaccgga aatctggttg ttttgcacct taatctggga aataacatcg gaaggtacca
     5161 ggttatattt attgagtttt tgcgggtcca gccagatacg catggcgtac tcggaaccaa
     5221 agagctgtac gctaccaacg cccgcggtac ggcttagcgg gtctttgata ttagacgcta
     5281 catagtccgc gatatcgtac tggttgaggc tgccgttatc agaaataaat gccgctacca
     5341 tcaggatatt actgctcgac ttatcgacgc taatcccctg ctgctgcacc gcttcaggta
     5401 atgaaggcat agcgagctgc agtttatttt gcacctgaac ctgtgcgata tcaggcgatg
     5461 tcccagtctc gaaggtcaga gtgatggagg cattacctgc cgcatcgctg gttgaagaca
     5521 tgtacatcag gccatcaagc ccattcatat tttgctcaat cacctgagtg accgagtctt
     5581 ctactgtttg cgcatcggca cccggatagg tagcgctgat ggtaatggtc ggtggcgcaa
     5641 tctgcggata ctgcgcaacc ggtaagttca tgatcgccag accacctgca agcatcataa
     5701 taatggcaag tacccaggca aaaaccgggc gatcaataaa atagttagcc atgaaagtcc
     5761 ccttaagcct gcaacgttat tgtttcgatt cggtgctggc gttttcctgg ctggaggaaa
     5821 ttgctcgtgc tttgataccc ggacgaatgc gttgcaaacc ggaaacgatc acccgatcgc
     5881 cggcctgcaa gccagaggtg acaacccact ggtcgccgat ggctttgctg gcttcaattt
     5941 cgcgtagctg cacgacatcg tctttatcca gaatgagtgc cgttgcttta ccctgggcgt
     6001 tgtgggtgac gccttcctgc ggcaccagta atacattctg gcggctacct tcatccacta
     6061 atgccgtgac atacatgcca ggcagcaagt caccatttgg gttggggaaa atcgcccgta
     6121 acgtcacgga gcccgtggtt tcatctaccg ttgggtcgga gaatttcagc gtgccagtct
     6181 ggctgtaacg tttaccattt tccagattga gctgtactgg cgtactgccc tgaacctgtt
     6241 tgatttgccc actggcgacc tcttctttca tgcgcaagaa atcctgcacc gactgcgtga
     6301 gatcgacata aatcgggtcc agacgttgta cggtaaccag cgaatctgcc tgattagcgg
     6361 taacgagtgc gccgacggtc accgacgatt tcccgctgac gcccgtaatc ggcgaggtga
     6421 catttgcgta ttgcaggttg atagtcgcct gttcaacagc cgctttggcg acggtgacat
     6481 tggcttctgc ttcattcaac tgggtgcgcg cggtgtcgta atcctgacgg ctaacgtagt
     6541 tggtcttcag caacgatgcc tggcggttaa aggtgatgcg ggcattgctg gcggtagaga
     6601 gcgctttcgc cagcgaacct ttggcggagt tcagctctgc ctgtaaaggc gcaggatcaa
     6661 tctgatacag cgaatcgccc tggttcactt tatcgccttc gataaagttg cgtttaatga
     6721 taataccgcc cacctgggga cgtatctcgg caacttcata aggaacggtt ctaccgggca
     6781 attcgctcaa cacattgacc gaaccggggg agagtgtgac gacaccgacc tcaggcgtca
     6841 tggcggcggc gttttccgcc gatttgtcat cgcaggcggt gagcatcgcg ccgcagaata
     6901 acaacggtat taacagtttt cttcttctgt tcattttagt ccctgaaaat tcttgagaaa
     6961 taacggttct ttaaattgcg cgcgaggaga gatatgaggg atttacagac cagcggcata
     7021 gtggggttgt cagccagtaa gatgggatgc aagcacaatg atcgacatgg tgaggtcaac
     7081 gacaaaaaaa atgttgccgt tctgccaaca gtttctgcca gcattcgggc aacgaagggt
     7141 tctactggtg gatacacata ccaggggaat aaagtcgttt tcgtctctcc ttcaataatc
     7201 accgggggta ttgaaatcag catctgcatc cctcgtcatg ccagccatca atttcagttg
     7261 cttatgtcct tactaaaaat aagatgtgat acccagggtg acgatgtcgc tcatacgtct
     7321 taatttcagc ttgtacataa tgttgaattt ctgaatcttt aatgtttgtt gcgaaatgtt
     7381 gagcgttgac gtgatattgc tttcattttt gtttttcaga atcagttgca taattttgaa
     7441 ctgattatgt gaaaaacata gttgtgaatt cttatggggc aagtgtttac cataagtaat
     7501 ctcaagaaga tttttgatac tttctttacg gctgacaaaa taaataggcg ctttagcttt
     7561 tagtttactg atgtgataag gtgccaaaac aataattctc tcggcatcta atttctccag
     7621 aaatttaatc gcttcttcat caaggatatg attgtgatag gtatcaatga taaccttgaa
     7681 gggtttattg cctacttttt tgatctctga cagggaattg atttttatca tttcgtgatt
     7741 atctttcaac tgccaaaagc cctgtaaaag aaaagaatct ttcgtcatga gaaaaatcat
     7801 aacttgctcc ttagccgtta tcgtttcgaa catgtcatcc ttttcattat ttacatcctt
     7861 gtccgaatcg ttgttcaata tagtaaacgc ctataagaac tttgcccttc gtgaactaat
     7921 acgtctatta acactaatca atacttcaga tttatccatc acgcactcca tatattgatc
     7981 ctaagtaaaa ttcctggttg ttatcaattt gtaaatgata aacaaaaagt taacgtttgc
     8041 ggattactgc ccaagaataa gttatcaata accagtttgt gatctctgaa gaatattact
     8101 aaagttaaaa tgtaatccga tttaaatatc gagtctcctt gtttcgactt aggctggcaa
     8161 ttggattgcc agctttcttt taacgcattg aaattacctg agtaaataac tcattcatgt
     8221 cttcaggttt ttatatagcg tctggagaca cggatattta tgcaatgtaa tcaacgccag
     8281 ctgcaaaaaa atgaacccgg attacttatt taaccgccta tagcatcaac gcttacagaa
     8341 ctctttgttc actttcccac agcactttta aaacattaaa cctgaccacg gaaaatatca
     8401 gccatgataa tgtttgaaat ggctaatttg ccatggaatg aaaaaatcag attaaattca
     8461 gtaggttgaa ataatcaaca gcaggcaatt gtttcaatga tagtgcgtaa ttgaactgaa
     8521 acactgtata gctaagagag tattaactgg atcatagtca cttaatgtga ctatgatcca
     8581 ggtggaaata gggataatta ttgcttttta acgaacaaac tggcgaagct gaacagactg
     8641 gcggcgctaa atatcagttc aattcccacc agtgtggaaa ccagcgttac cgacaccatc
     8701 ggcgtagcac caaggaatat ccaggcaatg acgatatcca gcacaccaat aacgagctgt
     8761 agccaactgc ctttcattga acgctgacga taccaactca tcaggcgaat aacccctgcc
     8821 acacagaaca aaccggcaat aaatgccgca atggcaaaaa tgcccagctc tggtgcgcgg
     8881 atgaagaaat agccgatcaa taaataggcg actgcgacga ggaaaccgga taataccggc
     8941 cagaaattat gactgcggtt gctgaataac ccgacaataa gcgcaatacc cgagcagatt
     9001 aataatgcac ccactactgt gcttaaaata tcgccagaga cgaacggaaa actgatacac
     9061 agcaacccga cgataaacag cagcacggca ataaactgga ttgctctgcg atgtttttta
     9121 agcatctcca gatcaaactt caaaattgtt gccttatcta tatataacat agaaccaccc
     9181 tataaaatta agaagaaaat cccctgctat caatctatgc caaaaacgcg tgtaagaatg
     9241 cagtcgattt aataaaaatt tcctaattac agtatctgat gcatctgtaa ctcattgtat
     9301 tgaaataaaa atatctgatt ttgatatttt ccatcaacat gacatataca gaaaaccagg
     9361 ttataacctc agtgtcgaaa ttgattcgtg acggctcttt cactttatag ttgaggatat
     9421 tacgatgaaa aaagtattag gcgttattct tggtggtctg cttcttctgc cagtagtgag
     9481 cagtgcagcg gatgcacaaa aagcagctga taacaaaaaa ccggtcaact cctggacctg
     9541 tgaagatttc ctggctgtgg acgaatcctt ccagccaact gcagttggtt ttgctgaagc
     9601 actgaacaac aaagataaac cagaagatgc ggttttagat gttcagggta ttgcaaccgt
     9661 aaccccagct atcgttcagg cttgtactca ggataaaaaa gccagcttta aagataaagt
     9721 taaaggcgaa tgggacaaaa ttaagaaaga tatgtaattc cgggaatgcg ttacatcgta
     9781 tttccttgca tattgaacag gccggaatat cttctttaaa agcagctatt cctcctgttt
     9841 gtatataatc tctatattga atgggttaca aaatgaacat tgcatctctc cgtaaagcgt
     9901 ttatttttat gggcgctgta gcggctttgt cactggtgaa cgcacaatct gcgctggcag
     9961 ccaatgaatc cgctaaagat atgacctgcc aggaatttat tgatctgaat ccaaaagcaa
    10021 tgacaccagt tgcgtggtgg atgctgcatg aagaaacagt atataaaggt ggcgataccg
    10081 ttactttaaa tgaaaccgat ctcactcaaa ttcctaaagt gatcgaatac tgcaagaaaa
    10141 acccgcagaa aaatttgtat accttcaaaa atcaagcatc taatgacttg ccgaattaat
    10201 aaggtacaag taaaaaggag tagcaagttg agccatcttg ctgctccttt ttgcattttt
    10261 atatgacagc agaatttatt atacgtctta tactcgcggc aattgcctgt ggcgctattg
    10321 gcatggaaag gcaaatgcgc ggcaaaggag cagggttacg cacacatgta ttaattggca
    10381 tgggaagcgc cctgtttatg attgtttcga aatatggttt tgctgacgtg ctgtctttag
    10441 atcacgtcgg actggacccc agccgtattg ctgctcaggt ggtgacgggc gtcgggttta
    10501 tcggtgcagg taacattctc gttcgtaacc aaaatattgt aggtctgacg acggcagcgg
    10561 atatctgggt gaccgccgcc attggtatgg ttattggcag cggtatgtac gaactgggta
    10621 tttatggttc agtgatgacc ttgctggtgc tggaagtctt ccatcaatta accttccgcc
    10681 tgatgaacaa aaattaccat ttacagctga cattagtgaa cggtaatacg gtatcaatgc
    10741 tcgactggtt taagcagcaa aaaataaaaa cggatttagt ttcgttgcag gaaaatgaag
    10801 atcatgaagt ggtcgccatt gatatccagc ttcatgccac aacgtcgata gaagatctgt
    10861 tgcgattatt aaaaggtatg gcaggggtga aaggcgtttc aattagctaa tctgtatagt
    10921 tatttattcg caggagtacc agtgcggtac tcctgttggt tttaatgatc gaggacgatc
    10981 agtggtgaat ggttggcagg atctttaagt acggtaatat ccgcgccgta gagcattgtc
    11041 agcgcctgct gtgtcaatac gtcctgcggc ttaccatttg cgataacctt tcctttttgc
    11101 attaatacaa cacggtctgc ataacgcgcg gcaagattta gatcgtgcaa tacgcagcaa
    11161 acattgaact gccgttcgtg aactaactgg cgtaataaac gaaacaagtg ctgctgatgg
    11221 tgaatatcca gcgccgaagt tggttcatca agaaataacc atttcggtga gggagtaggt
    11281 tcccagagtt gcaccagcag gcgcgctaat tgcacgcgct gttgttcacc accggataat
    11341 tgccgatagt cgcggtttgc taacgcctga cagtcacata acgctattat ttgtgctgtt
    11401 tcattatcct gattaccagt acgatgcgga tgccgcccca tctggataac ttcttgtacg
    11461 ctgaatggaa acgccatgtg gctattttga cgcatcacgg cccggtgttt cgccagttca
    11521 gtaatcgacc attcatttaa tggcttatta aacaaccgac attcgccgct atcaggttga
    11581 agatagccgg ttagctggcg aagcagcgta gattttcccg caccattagg gccgaggatg
    11641 gcaacaattt ccccaccggg aaaagtcagc gagacattat cagtcagacg ccgcccctga
    11701 aggctataaa ccagattctg ggcgctgatc atgtgatcct ccggttgcgc agaatcaacc
    11761 acataaaata agggccacca agcaggctgg ttaataatcc cacgggcatt tctgctggct
    11821 gtaccagcgt gcgggcaagc gtgtctgcca tcagcaataa acaggctccg gcgagggcgg
    11881 aacaagggat tagccagcgg tgatttgccc cggtagtcat gcgaatcaga tgcgggatca
    11941 ccaggccaat aaagccgata atgccgctta ccgatacggc ggcaccaacg agcagtgagc
    12001 tcactaacag taattgctgg cgtttacgct taacgttcac gccgaggtag tgggcttctt
    12061 catcaccgag ctgcagtaaa ttcagcgttc cggcgagaca agttgttgcg ataatggccg
    12121 gtaggatgaa tgaactggca accaataacg tcggccattg cgcctgtcca agattgccca
    12181 tcatccacaa tgtgagttgt cgtagctgct gctcatcgcc gatatagctc aggatgccga
    12241 tcgctgcgcc gcacagggca ttgatggcga taccggcgag taataattgc atcatgctgc
    12301 catcgcgatg gcgctgcgtg atgagaaaaa tgatggtgca gaccactaaa cttccggcaa
    12361 tggcaaacac catttgctca tagagcacca gcaccactgg gaaggagaag ggcagcacaa
    12421 tagcaacgcc aaccattaat gcagaaccgc tactgacgcc aagtaatcca gggtcagcca
    12481 ttgggttgcg aaaaagccct tgcataatta cccctgcggt tgccaacgct gcacctacca
    12541 gcaccgccag caaaaccctt ggtagacgaa tattgagcca gatatcgcgg tattcctcat
    12601 caaaaagggc atcaaaactg atctttaacg ctccctgact ggcaccaaac agcaccagag
    12661 cgagcaataa tagtgatagc cccataaaga ccctggcgga agagagtgaa tccttgagca
    12721 cgcaaaactc cgttacgaca ttacggcgcg gacataccgc gccgggggat cacgccggtt
    12781 tcaggtccgg ttcaatcaga ctgtagacct gctggttaag tgctggcgca tttgccagtt
    12841 catggatatg tgcggcgaca tcggcacggt gaacaaaacc atgacactcc tgattttgta
    12901 tccgttgtgc tttgcccgtt gccgccccat caagcagacc gccagggcgg agaatggcat
    12961 aatcaagctg gctggtttgc aaccagcttt ccgccagcgt tttttcccgt accgcctgac
    13021 caaacgcagc ctttgcccgc tctgataaaa acggccagct atcgccgcat cccaaagagg
    13081 tcaccagaat catgcgactg atgccagcct tctcggcctc gtcaatcact gttctgtgcg
    13141 ccagataatc ctgagctccg cccattgttg agatgataag cgcatccggg ccagctgcgc
    13201 ggcaagcagc cgcgactacg ctggcatcac aggcgtcgcc tgtaaaaacc tgtacgcctt
    13261 gttgcgccag tttcgtggcg gcatcagcat gacgaatgac agcaacgacc ggacgttgtt
    13321 ccgccagcgc cagttcaagc gttctggcac ccacaccttt tccgcctgcg ccaaagagta
    13381 gccacggcgt catcaggcgt gttcctttaa cgatgcttcc agagcatgga aggcgctgac
    13441 ctgttcgctc aggagctgac gatggtcatc cctgccgagg aagattttca gcattgcgct
    13501 gccttctttg ttgaaaaaga gaatggatgc ggtgtccatg cccataaatt tacgttcgat
    13561 caaggcaata tgtgtgcagt tttctgcttt gatatgcccg gacatgccgt gctttccgcg
    13621 taggttgaaa tagccgtggc ggtggaaacc cgaaggcagt tcgccgctaa attcgaggat
    13681 gacatcggca gtatgtacta acgtggtgac gttgccccat tcgcagacgg tatcccagac
    13741 agtatcgaat ttatcgcctg acactatggt agaggagggc agatttttca ctacttccag
    13801 cagcgtggtg ttgtattgtt cggctaccac ctccagcgta ccgtccggct ccgttttcaa
    13861 aaattcttgt aacgagacat ggctcataaa tcactcctgt tatggttttt cacgcacaat
    13921 cgctggcgca ttaagtacct gaattagttc attaagagac tgcaaaatat tgcttgccca
    13981 gaaacgacct tcattagtca gccgcaggca agttgacgca tcgcggctta agcctttttg
    14041 gtgccattga gccagtaacg gcgcgagttt ttcggcatgt ggcgtaagtt cgtccagcgg
    14101 tacacgcgct gtttcaacac ctgactgcaa ggtatgacgc cattgcgcat cacgttcggc
    14161 gttacgcatg atcatcatca gcggtttttt gcctgccgcg acggattcat gccaggtctg
    14221 aagattgcgt tcgttcatcc aggagtaacc attaatcgaa ccaccggctc cggaaccaaa
    14281 ggccagacaa tcggcacctt gttttatcag caggttatag agattgcgtt cgcgtgtggt
    14341 acggccccag tggctgttac tgatgcagcg ccaaccggca tcgtccataa aatcacaccc
    14401 ttgcaggtaa agatcgcgac gttctgccgg agagggcacg gtagtacgcc cattttccac
    14461 ggctttgccc agcggtgtat tgggcaggac attgagcgca tagagatcga cgccgtcgag
    14521 accgatatcg cgggcaatag ccagatcttc tccccaggtt tgcgcatcct gacccggcag
    14581 accaaacagc aggtcacaga ccaccgcggc acggtcgcgt ttaaccaggc tttccataaa
    14641 cgcaatagcg gttgggccat ccgaggtgcg ggccattttc ttgcggattt tgctgttaaa
    14701 cgactgaata ccaattgaga agcggtttgc tccagcatca aggcaagcat cgattcgctc
    14761 agcgtcaaaa ttcagtaccc ggccttcgat agtgatttca caatcagggg ccagtggcag
    14821 cttttctcgt aatgtggtga tgattctggc caaatcgtgt gccgaaagcg cggaaggcgt
    14881 accgccaccg aaatagaccg cgtggatggg ggcagactga tgcaatacgc tgtctgcttc
    14941 cagttctatt tcacgaatca gggcgtcggt ataatgagca cacgcatctt cattaaaacg
    15001 gttctgataa aaaccacaaa acgtgcagtg tgtcgcacaa aaaggaatgt gtaggtaaac
    15061 cagtcgctta cgtggtgata ccgtttgatt gatcatctct tgccaggtct gtgccagttg
    15121 ctctttggca accggaatgg ccccacgaaa cggcatcatg gcgcgcctgt ctttaaaggg
    15181 ctgatcaccg tctaaagcaa aatgtggcgt gagatccagg gtgttatttg tgttcattcg
    15241 tctgatttaa atccatttat tgcggccaaa gctgctggtg taaagactca acgacatcag
    15301 caatacgcgg acccattccc agaatcaggt tttgatcaac ggtgataatg cgctggttct
    15361 tccaggcggc ggtgtgggta attccggcaa tagaacgtag ccgattaata tcgccatcga
    15421 ccatttgtga ggtgacgaca atcacttcag ggttagccgc aatcagcgat tccgcgctgt
    15481 agcttttgta ctgctggtgg gtagcgacgt tttctgctcc ggcaagcgaa agaatggcgt
    15541 ccgcgacgct gcctttaccg gcaacctgcg gcgcacttcc gcctgcggag agaataaaca
    15601 tggctttaac cggggctttt ttggccgcca cgttttgctg tactcgctcc aggcgttgat
    15661 tgatctgtgt caccagcgcc tcgccttgtt caggaacctg tagcgttttt gccagttggc
    15721 gaatgttggc gtacatctgc tcaagggtgg caggcacacg cggcagggtg acgacgttga
    15781 ctttttgcgc ccgcagttgg tcgagcacaa tttgcggtcc tgcatcctgc caggtaatta
    15841 cgctatccgg gcgaagtgac aaaatgcctt cgctgctgag ttgtttccag taaccaatat
    15901 gcggcagtct ggcggtttct ggtggataag atgtcgtttc atcgacacca accacgcgtt
    15961 tgccagcgcc catcgcgtag attagctccg tcagcgatcc tcctgcgacc acgatacgtt
    16021 cggcagccgt tacgctaaaa gtccagctaa gagccagtaa tgccgtaaac aatttttttc
    16081 gttttaccat ggtcttcgcg gcagaggcca cggctgaaat acataacgtc agtggggaaa
    16141 aaagaaacgg cctggtgatg atccttggta taatatgaga acccttatca aattgagaat
    16201 gatcatgatt atcatttgag ttatatatga aatacaaccg tcgattcaat acgcacggcg
    16261 ttacaagcgt atttattttt gatttttatc aagatgcttc gtcagctaat gttcttgtta
    16321 ccgattaaat ttcagcgcgg gaaaaagctg agaacccgga gatatcgcgg cttgcagagc
    16381 aaagagaaga gaaaattatc actgttaacg acaacgtttt gtgatgaggt tgtgggggga
    16441 tagccataaa cacaggatgg ttttctggat gctggataga tttcataacc ccctgtttat
    16501 tgtgttaatg gcggttaaaa aaagcttgat cataaaaaat tgatcgataa taattctcat
    16561 tatcacgttt gagtttaaca aaatatgaca tcatggataa caaaatcata tcttttgtaa
    16621 gggaaaacgc acagcactct tcacttcaga cgtggcgtac tactttgcct gacaaagagg
    16681 gcgtgctttt gctgaattat cagaaatatt cggcaatttt acgggatata tacgctaata
    16741 gcttcccgtg gtgatatcta atcatttgaa ttgtaaataa atttattttt cgccagccac
    16801 cccggtatta acccaggcag ccagcatttt ttattttcct catggagaat cgctatgtca
    16861 cgtccgcaat ttacctcgtt gcgtttgagt ttgttggctt tggctgtttc tgccaccttg
    16921 ccaacgtttg cttttgctac tgaaaccatg accgttacgg caacggggaa tgcacgtagt
    16981 tccttcgaag cgcctatgat ggtcagcgtt atcgacactt ccgctcctga aaatcaaacg
    17041 gctacttcag ccactgattt gctgcgtcat gttcctggaa ttactcttga tggtaccgga
    17101 cgaaccaacg gtcaggatgt aaatatgcgt ggctatgatc atcgcggcgt gctggttctt
    17161 gtcgatggtg ttcgccaggg aacggatacc ggacacctga atggcacttt tctcgatccg
    17221 gcgctgatca agcgtgttga gattgttcgc ggaccttcag cattactgta tggcagtggc
    17281 gcgctgggtg gagtgatctc ctacgatacg gtcgatgcaa aagatttatt gcaggaagga
    17341 caaagcagtg gttttcgtgt ctttggtact ggcggcacgg gggaccatag cctgggatta
    17401 ggcgcgagcg cgtttgggcg aactgaaaat ctggatggta ttgtggcctg gtccagtcgc
    17461 gatcggggtg atttacgcca gagcaatggt gaaaccgcgc cgaatgacga gtccattaat
    17521 aacatgctgg cgaaagggac ttggcaaatt gattcagccc agtctctgag cggtttagtg
    17581 cgttactaca acaacgacgc gcgtgaacca aaaaatccgc agactgttga ggcttctgat
    17641 agcagcaacc cgatggttga tcgttcaaca attcaacgcg atgcgcagct ttcttataaa
    17701 ctcgccccgc agggcaacga ctggttaaat gcagatgcaa aaatttactg gtcggaagtc
    17761 cgtattaatg cgcaaaacac ggggagttcc ggcgagtatc gtgaacagat aacaaaagga
    17821 gccaggctgg agaaccgttc cactctattt gccgacagtt tcgcttctca cttactgaca
    17881 tatggcggtg agtattatcg tcaggaacaa catccgggcg gcgcgaccac gggcttcccg
    17941 caagcaaaaa tcgattttag ctctggctgg ctacaggatg agatcacctt acgcgatctg
    18001 ccgattaccc tgcttggcgg aacccgctat gacagttatc gcggtagcag cgacggctac
    18061 aaagatgttg atgctgacaa atggtcatct cgtgcgggga tgactatcaa cccgaccaac
    18121 tggctgatgt tatttggctc atatgcccag gcattccgcg ccccgacgat gggcgaaatg
    18181 tataacgatt ctaagcactt ctcgattggt cgcttctata ccaactattg ggtgccaaac
    18241 ccgaacttac gtccggaaac taacgaaact caggagtacg gttttgggct gcgttttgat
    18301 gacctgatgt tgtccaatga tgctctggaa tttaaagcca gctactttga taccaaagcg
    18361 aaggattaca tctccacgac cgtcgatttc gcggcggcga caactatgtc gtataacgtc
    18421 ccgaacgcca aaatctgggg ctgggatgta atgacgaaat ataccactga tctgtttagc
    18481 cttgatgtgg cctataaccg tacccgcggc aaagacaccg atactgggga atatatctcc
    18541 agcattaacc cggataccgt taccagtacc ctgaatattc cgatcgctca cagtggcttc
    18601 tctgttgggt gggttggtac gtttgccgat cgctcaacac atatcagcag cagttacagc
    18661 aaacaaccag gctatggcgt gaatgatttc tacgtcagtt atcaaggaca acaggcgctc
    18721 aaaggtatga ccactacttt ggtgttgggt aacgctttcg acaaagagta ctggtcacca
    18781 caaggcatcc cacaggatgg tcgtaacgga aaaattttcg tgagttatca atggtaatca
    18841 tctgccccga tatttcgggg catttatctg gaaggaagag agacaatgaa ccactacaca
    18901 cgctggcttg agttaaaaga acaaaatccc ggaaagtacg cgcgtgacat cgcaggttta
    18961 atgaatatca gcgaagcaga actggcattt gcgcgcgtca cgcacgacgc gtggcggatg
    19021 cgcggcgata tccgtgacat tctggcggcg ctcgaaagtg ttggcgagac caaatgtatt
    19081 tgccgtaatg aatatgcagt ccatgagcaa gttggtgcgt tcacaaacca gcatttgaac
    19141 ggacatgccg gattgatcct caatccgcgc gcgctggatt tacgtctgtt tctcaatcaa
    19201 tgggccagtg ttttccacat caaagaaaac acggctcgtg gcgaacgcca gagtattcag
    19261 ttctttgatc atcagggcga tgcattacta aaagtttatg ccaccgacaa taccgatatg
    19321 gcggcatgga gtgagcttct ggcacggttt atcaccgatg agaatacgcc gcttgagtta
    19381 aaagccgttg atgcgccagt tgttcaaacg cgagccgatg ccagtgtggt cgagcaagag
    19441 tggcgggcga tgaccgacgt tcatcagttt tttacgttgc tcaagcgcca caacctgacg
    19501 cgccaacagg cgttcaatct ggtggcagac gatttggcct gcaaagtatc caacagtgcg
    19561 ttggcgcaaa ttcttgaatc tgcacagcag gatggcaatg aaatcatggt gtttgttggc
    19621 aaccgtggct gcgtacagat tttcactggc gtggtagaaa aagtggtgcc aatgaaaggt
    19681 tggctgaata tttttaaccc gacgtttact cttcatctat tagaagagag cattgctgaa
    19741 acctgggtaa cccgtaaacc tgctagtgac ggttatgtga ccagtctgga attgtttgcc
    19801 catgatggta cgcagatagc gcaactttat ggtcaacgta cagaaggcga acaggagcaa
    19861 gcgcaatggc gtaagcaaat tgcttcgctg ataccggaag gcgttactgc ataacgtata
    19921 attaacggga ggtctggcta tttattgctg ggcctcttta tattaatcaa tcgatcacac
    19981 cagataatca atatgctgat ggcgtaccaa ctcattgatc cgcttaagat tgaggatcat
    20041 catgatattg tgtttatggc agtagaacgt ttttagcgat attttcagtt tctccagaat
    20101 atcttccgtt gtcatttgat tcgcaatgca gctcagaacc tgatgctgct tgcgcgaaag
    20161 ggccacatcc gcttcaggca cagagcagta actgccattg atgagtgcga caaagtcgat
    20221 gatggtgata gttcgcggaa caaaggtcac cggtactttc cccaggcagc gtttaatatt
    20281 aaagggagag aaaataatca catggacagg ttttaatcgt tcaatttgag tgagcaaatt
    20341 agaatgaaat acattattca ttaatgtatc aataatgacg aaggcatgct gatgcaacat
    20401 tacgtcgatc tcttcttcgt tctgtatatg aacgacatta cctttactca gaatgttttt
    20461 catcgcagtg aagaacatcg tatccctggt aattataaga aacataaccg actcctggtg
    20521 cggacattaa ctttataatc agtttgccaa aggtaattaa caggacaggg cttataataa
    20581 taattagctt actgtgtatt aatttgatgt aatgcatgat gcagatgctg agaaagcatc
    20641 tgcatctttc ggtggtgtta tttgaccagc tcaggtgtta cctgactcac cgcgttggtg
    20701 tagtaaggcg caccccagcc cggttccggc cagaatgcac catagccgta atcccacagg
    20761 ttataggtgg tattaaccac ttctctcaaa tgccacacct ggatgccctg catattcact
    20821 tcgaggaagt tatacgggac tttattgata aagccaggtt gttcaccctg aatggtaccg
    20881 aggatggtaa caaagtgatt acgatagttc actggatcaa ggaagccgct ttgtctggcg
    20941 agcagtcggc cctgatagtt ggcttcaata tcaggcttcg catagctatc caacggtaat
    21001 acagcgattt ctaacaacgt atccgttttg ccattgataa cgttgataac cttcccacca
    21061 aagcgcgctt gttgaccaac atataacccc ggctggttat gaacagcaac aaaacttttt
    21121 tgaatatcag gttggttatt acctttgata ttttgcggaa ttgaactaca tgcggcaagc
    21181 aaaaatgaaa ggctgaggat gagtgcacct tttgtcatgt tcatgttact atccttatca
    21241 acaaatatta aaaccataat agatatttct ttatagtttt catctgattc tgaggcgata
    21301 tgcctgatag taacgtaaac ctgtctgatg tttatatccc ttctgtttag tttaattctg
    21361 ttaattttgt ggcgtgacat gagtatccat cgccccataa aatagccttt ttttaaaggt
    21421 gcataaaatc tatttttatt caatgcttat aaagcgctaa tgttcaatat tatgggatta
    21481 tattctggta atgatttgat aagcgaattc ttttgccaat tggtgtttct atttctaccc
    21541 cataatttat agttttttca tcaggtagcg atctctttaa ataaggtgcg tatgacattt
    21601 ttttaggatg aaacgtatcg tgttgaaata tttcgaattg atattcagac atttgtgccc
    21661 attttttaat tctattgtat cgagagggtg ttgtaaaatc tttaacttgc ttgtaacatt
    21721 ctttttggaa ttcttgctga tattttatta tgtcgttgta tgaaagattg tttaccctgg
    21781 tttctaataa ttctattatt gtcgaagcaa tttccgcttt tgctcttaaa gagttgtcat
    21841 taatggttaa taatgttgaa aataaaccat tgatttgttt gtcaacatat tgttcatctt
    21901 tatgtaattt tgtgatgggg ttttccttat gtgaaaaaat agtatcaaat aaatttgcgt
    21961 cagtgaagtt tacattaagt agatcttcga aataaaacct tgacaaggtt agaacgaacc
    22021 cggacaggtc ctgtcctgtc atattgaaat caataaagtt ggttccagca aagactttca
    22081 tcctgttttc attttcactg tgctcaatat acaaccttgt cattttttgg tacaagcgtt
    22141 gaatatcttt tatatcagtt ggtaaatcag ttcgtagccc agaaaaattt ttattttcgg
    22201 aagaaacggt ttggacactg aatcttactg gtattccctc ttctacgaga attttagtta
    22261 tgattggctt attgggctta ggattaaaga caacaatgat tttagattca gagtttttat
    22321 tcttttcgta tctaatgtta ttaatctgat taagtattga ttgtgtctgt tgcgtaagtg
    22381 gtgtcgaact tatcgtttca atgttattaa taagttttac aagttgagcc acaacttttg
    22441 agtagtctgc atttgtatat gtggtgttcg tgtggcacgc attaatttgt tggagttgtt
    22501 gttgcgctaa atcaaattgt tcagcgttat atccttcttc agacaatgaa aaattaatgg
    22561 gttgctgttc ttcctgatta tcacaaggcc acatcggtgt gtctggtgtt ttaacattgt
    22621 cggtatctgt tgtgcggtta taaatatcgc cgtgatatgt atttatcata cctggggtaa
    22681 agtcaattga cataacattc cttattgttc atctatttcg ctaaattttt gtaaatcgga
    22741 gaattagagc aggaatggat tttcttctac gcggaaataa acacaatgcg tcatatcttc
    22801 ctgatataat tacctttaac ttccctttca tttccacccg ataatatttg tgtataaaaa
    22861 aagggcagtg atagtatcgt aaaggaaaac attaacaact atcaaaaagc gctcatttta
    22921 agaagttatg atgttctccg atagtgtgtg aagtgatatt tttgtgatgt tttagatgtg
    22981 taataatgaa ccaggttaaa gtgtatttaa aaatgtgttg tattggcgac tgaaatctac
    23041 cctttaaaag ggggatgata ctgagacaaa gagacattgc ttctccgata aaatgtcaga
    23101 gaagcatgat tgcgtggtga ttgtcgattg atgacttact gacggatccg tcatcattct
    23161 caaagcatta gttaagttat ttgttatttt taaggattat cctatctatg attttctact
    23221 cagaaggata aggcactcac aatactcctt atagtttact gctggcatct atttaaaata
    23281 gcatttgagt caaatagccc atcaagatag ccatacctaa tataaccgtc aggaatgcaa
    23341 ctatcatcgg tattctgaac attgatttaa gtaatatcac ttccgtcagg cttgcgccgg
    23401 cactgccgat gattaatgcc attaatgctc ccagacccat tccttttgtc atcaaaacag
    23461 atgccagagg gataactgcc tctgcccgga tatatagcgg aataccaaca acggcgctca
    23521 atgggatggc gaagggatta tctgcccctg catgagcggc aatccactct gagggaataa
    23581 agccataaat aaaagagcct attaataccc ctaacaaaag atatggcagt acatctttaa
    23641 actgttgcaa cgcatctttg gtggctagtt ttatgggatt aatacttacc acagctttgg
    23701 tattgcaaca gttaactacg ggtttctcaa tggctttagc cgttggacta cagcaactca
    23761 cttctattgg cgtatatgtc gtccccggcg aagttttggc tggagcacaa caatttgctg
    23821 atgagctttt actggcaatg atatgacgtt caaatcccag ggaatccagg ataatactgg
    23881 caagtacgga gacgccggcg gcgataatcg cgtacaacaa ggtaactttc cagccaaagg
    23941 tcacccacat taacccgacg ataatgggat taagtaatgg ggaaacaaat aaaaaagtga
    24001 gggtcggacc aaacccggct ttcgctgata acaatccacg taacatgggg attgtcgagc
    24061 aactacagaa cggggtaacg gctcccaaca gagcagccag gagataacct ctcccttttc
    24121 tggcccccat catctgctgg attttatggt ctggcacctt ttgtcttatc aggctgacac
    24181 cggcactaat cactataaac agcaaagaaa gctcgacggc gagaaacaca aacatctctg
    24241 cggcatcttg cagcatcgca agccaactac tcatacgtaa cctcattgaa taaatctata
    24301 tttccagaat aatcgaaatg aaattcagat caagatattt ctggtattat cgaaatatca
    24361 accagacaga ggggagtcat atgcagttgg aagaagttgc taaagcgctg aaagagctag
    24421 gtcacccgac ccgtttgttc atatttaagc atctggtcaa agcgggggaa caaggtttgc
    24481 cggtcggtga gttgcaaaag cagcttggga tacccggctc tacactcagt caccatattt
    24541 cagcactggt atcggtaggg ttggtcaaac aaaaccgtga gagtcggacg ctgatgtgtg
    24601 tttcgcaata cgcaatgctg gaagcgataa ttgaattttt acgtgaggag tgctgcgtaa
    24661 acagcaaaac agatggcgaa ggagccacga agaacaaata agggggcata tcccccttta
    24721 gctatttcag gcgtttaccc gcctcatcaa ccactttctc gccatcttcc ttggtgaacg
    24781 cgcgttgttg cgcatctggc agaatttcca gcaccacttc tgaagggcgg cacaggcgag
    24841 ttcccagcgg tgtcaccaca atcgggcggt taatcaggat gggatattgc agcataaagt
    24901 cgattaaccg atcgtcagta aatttatctt ctgcaaggcc cagttcctca tacggctcaa
    24961 cgtttttacg caacagcgca cgaacggaaa tccccatatc ggcaatgagc ttgatcagtt
    25021 catcgcgtgt gggtggagtt tccagataat agataatggt cggttctgta ccgctgttgc
    25081 gaatcatctc cagcgtatta cgcgacgtgc cgcaggccgg gttgtgataa atagtaatgt
    25141 tgctcatatc agtatctcat tacaaagtga aagagagacg aagcgccagc gcggcgagcg
    25201 tcacaaacag caccggcagg gtcatgataa tccctgtacg gaagtaatat ccccagctga
    25261 tagtcatatt cttctgcgaa agtacgtgca gccagagcag cgttgccagg ctaccaatcg
    25321 gtgtaatttt cggccccaaa tcgcagccaa ttacgttggc ataaaccatc gcttctttga
    25381 tgacaccaga tgccgtgctg ccatcaatgg acaacgcgcc aaccagtacc gtcggcatat
    25441 tgttcataat agaagagagg aaggcggtga
//
LOCUS       DAMZFT010000069        25461 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.069, whole genome shotgun sequence.
ACCESSION   DAMZFT010000069 DAMZFT010000000
VERSION     DAMZFT010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 25461)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25461)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25461
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.069"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(284..646)
                     /gene="ssrA"
                     /locus_tag="PN977_002882"
     tmRNA           complement(284..646)
                     /gene="ssrA"
                     /locus_tag="PN977_002882"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(861..1343)
                     /gene="smpB"
                     /locus_tag="PN977_002883"
     CDS             complement(861..1343)
                     /gene="smpB"
                     /locus_tag="PN977_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HDI6042900.1"
                     /translation="MTKKKAHKPGSATIALNKRARHEYFIEEEFEAGLALQGWEVKSL
                     RAGKANISDSYVLLRDGEAFLFGANITPMAVASTHVVCDPTRTRKLLLNQRELDSLYG
                     RVNREGYTVVALSLYWKNAWCKVKIGVAKGKKQHDKRSDIKEREWQVDKARIMKNAHR
                     "
     gene            1514..1951
                     /gene="ratA"
                     /locus_tag="PN977_002884"
     CDS             1514..1951
                     /gene="ratA"
                     /locus_tag="PN977_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010376629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin RatA"
                     /protein_id="HDI6042901.1"
                     /translation="MPQISRTALVPYSAEQMYQLVNDVQSYPQFLPGCTGSRILESTP
                     GQMTAAVNVSKAGISKTFTTRNQLTSNQSILMNLVDGPFKKLIGGWKFTPLSQEACRI
                     EFHLDFEFTNKLIELAFGRVFKELAANMVQAFTVRAKEVYSAR"
     gene            1941..2231
                     /locus_tag="PN977_002885"
     CDS             1941..2231
                     /locus_tag="PN977_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417108.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfH family protein"
                     /protein_id="HDI6042902.1"
                     /translation="MPGKIAVEVAYALPEKQYLQRVTLQEGATVEEAIRASGLLELRT
                     DIDLTKNKVGIYSRPAKLSDTVHDGDRVEIYRPLIADPKELRRQRAEKSANK"
     gene            complement(2293..2634)
                     /gene="bamE"
                     /locus_tag="PN977_002886"
     CDS             complement(2293..2634)
                     /gene="bamE"
                     /locus_tag="PN977_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HDI6042903.1"
                     /translation="MRCKTLTAAAAVLLMLTAGCSTLERVVYRPDINQGNYLTANDVS
                     KIRVGMTQQQVAYALGTPLMSDPFGTNTWFYVFRQQPGHEGVTQQTLTLTFNSSGVLA
                     NIDNKPALSGN"
     gene            complement(2783..4444)
                     /gene="recN"
                     /locus_tag="PN977_002887"
     CDS             complement(2783..4444)
                     /gene="recN"
                     /locus_tag="PN977_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HDI6042904.1"
                     /translation="MLAQLTISNFAIVRELEIDFHSGMTVITGETGAGKSIAIDALGL
                     CLGGRAEADMVRTGAARADLCARFSLKDTPAALRWLEENQLEDGHECLLRRVISSDGR
                     SRGFINGTAVPLSQLRELGQLLIQIHGQHAHQLLTKPEHQKFLLDGYANETSLLQEMT
                     ARYQLWHQSCRDLAHHQQLSQERAARAELLQYQLKELNEFNPQPGEFEQIDEEYKRLA
                     NSGQLLTTSQNALALMADGEDANLQSQLYTAKQLVSELIGMDSKLSGVLDMLEEATIQ
                     IAEASDELRHYCDRLDLDPNRLFELEQRISKQISLARKHHVSPEALPQYYQSLLEEQQ
                     QLDDQADSQETLALAVTKHHQQALETANALHQQRQHYAEELAQLITDSMHALSMPHGQ
                     FTIDVKFDEHHLGADGADRIEFRVTTNPGQPMQPIAKVASGGELSRIALAIQVITARK
                     METPALIFDEVDVGISGPTAAVVGKLLRQLGESTQVMCVTHLPQVAGCGHQHYFVSKE
                     TDGAMTETHMESLDKKARLQELARLLGGSEVTRNTLANAKELLAA"
     gene            complement(4530..5408)
                     /gene="nadK"
                     /locus_tag="PN977_002888"
     CDS             complement(4530..5408)
                     /gene="nadK"
                     /locus_tag="PN977_002888"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) kinase"
                     /protein_id="HDI6042905.1"
                     /translation="MNNHFKCIGIVGHPRHPTALTTHEMLYRWLCAKGYEVIVEQQIA
                     HELQLKNVKTGTLAEIGQLADLAVVVGGDGNMLGAARTLARYDIKVIGINRGNLGFLT
                     DLDPDNAQQQLADVLEGHYISEKRFLLEAQVCQQDCQKRISTAINEVVLHPGKVAHMI
                     EFEVYIDEIFAFSQRSDGLIISTPTGSTAYSLSAGGPILTPSLDAITLVPMFPHTLSA
                     RPLVINSSSTIRLRFSHRRNDLEISCDSQIALPIQEGEDVLIRRCDYHLNLIHPKDYS
                     YFNTLSTKLGWSKKLF"
     gene            5531..6121
                     /gene="grpE"
                     /locus_tag="PN977_002889"
     CDS             5531..6121
                     /gene="grpE"
                     /locus_tag="PN977_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HDI6042906.1"
                     /translation="MSSKEQKTPEGQAPEEIIMDQHEEIEAVEPEASAEQVDPRDEKI
                     ANLEAQLAEAQTRERDGILRVKAEMENLRRRTELDIEKAHKFALEKFINELLPVIDSL
                     DRALEVADKANPDMSAMVEGIELTLKSMLDVVRKFGVEVISETNVPLDPNVHQAIAMV
                     ESDDVAPGNVLGIMQKGYTLNGRTIRAAMVTVAKAK"
     gene            complement(6156..6761)
                     /locus_tag="PN977_002890"
     CDS             complement(6156..6761)
                     /locus_tag="PN977_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytoplasmic protein"
                     /protein_id="HDI6042907.1"
                     /translation="MSEDYLIEWDKDFADDLNVVASVFLQHTPTLWPTIFSQLSTQPE
                     IFEDEDEDEYGLQDVLDCSGGDLGNHDLAQAFLQVLRGEGLIQLVDWKGEDEEGELAN
                     FAADRFYELTKNLTASEELRSLLVEITQEDEISDVCEAGDRYLDEIFERIQTELNKRG
                     FQIFDLNEGSDTYNVVVLPMNEYKKIDDFNTPWLEVQDFLS"
     gene            complement(6885..8171)
                     /locus_tag="PN977_002891"
     CDS             complement(6885..8171)
                     /locus_tag="PN977_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026171.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HDI6042908.1"
                     /translation="MEHISTTTLIIILIIMVVISAYFSGSETGMMTLNRYRLRHMAKQ
                     GNRSAKRVEKLLRKPDRLISLVLIGNNLVNILASALGTIVGMRLYGDAGVAIATGVLT
                     FVVLVFAEVLPKTIAALYPEKVAYPSSFLLAPLQILMMPLVWLLNAITRMLMRMMGIK
                     TDIVVSGSLSKEELRTIVHESRSQISRRNQDMLLSVLDLEKMTVDDIMVPRSEIIGID
                     INDDWKSILRQLSHSPHGRIVLYRDSLDDAISMLRVREAWRLMSEKKEFTKETMLRAA
                     DEIYFVPEGTPLSTQLVKFQRNKKKVGLVVNEYGDIQGLVTVEDILEEIVGDFTTSMS
                     PTLAEEVTPQNDGSVIIDGTANVREINKAFNWHLPEDDARTVNGVILEALEEIPVAGT
                     RVRIGEYDIDILDVQDNMIKQVKVFPVKPLRESVAE"
     gene            complement(8192..8983)
                     /locus_tag="PN977_002892"
     CDS             complement(8192..8983)
                     /locus_tag="PN977_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417102.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YpjD"
                     /protein_id="HDI6042909.1"
                     /translation="MPVFALLALVAYSVSLALIVPGLLQKNGGWRRMAIISAVIALVC
                     HAIALEARILPDGDSGQNLSLLNVGSLVSLMICTVMTIVASRNRGWLLLPIVYAFALI
                     NLALATFMPNEYITHLEATPGMLVHIGLSLFSYATLIIAALYALQLAWIDYQLKNKKL
                     AFNQEMPPLMSIERKMFHITQIGVVLLTLTLCTGLFYMHNLFSMENIDKAVLSIVAWF
                     VYIVLLWGHYHEGWRGRRVVWFNVAGAVILTLAYFGSRIVQQLIS"
     gene            9150..10511
                     /gene="ffh"
                     /locus_tag="PN977_002893"
     CDS             9150..10511
                     /gene="ffh"
                     /locus_tag="PN977_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HDI6042910.1"
                     /translation="MFDNLTDRLSRTLRNISGRGRLTEDNVKDTLREVRMALLEADVA
                     LPVVREFINRVKEKAVGHEVNKSLTPGQEFVKIVRNELVAAMGEENQTLNLAAQPPAV
                     VLMAGLQGAGKTTSVGKLGKFLREKHKKKVLVVSADVYRPAAIKQLETLAEQVGVDFF
                     PSDVGQKPVDIVNAALKEAKLKFYDVLLVDTAGRLHVDEAMMDEIKQVHASINPVETL
                     FVVDAMTGQDAANTAKAFNEALSLTGVVLTKVDGDARGGAALSIRHITGKPIKFLGVG
                     EKTEALEPFHPDRIASRILGMGDVLSLIEDIESKVDRAQAEKLASKLKKGDGFDLNDF
                     LEQLRQMKNMGGMASLMGKLPGMGQIPDNVKSQMDDKVLVRMEAIINSMTMKERAKPE
                     IIKGSRKRRIAAGCGMQVQDVNRLLKQFDDMQRMMKKMKKGGMAKMMRSMKGMMPPGF
                     PGR"
     gene            10648..10896
                     /gene="rpsP"
                     /locus_tag="PN977_002894"
     CDS             10648..10896
                     /gene="rpsP"
                     /locus_tag="PN977_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000256453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HDI6042911.1"
                     /translation="MVTIRLARHGAKKRPFYQVVVADSRNARNGRFIERVGFFNPIAS
                     EKEEGTRLDLDRIAHWVGQGATISDRVAALIKEVNKAA"
     gene            10915..11463
                     /gene="rimM"
                     /locus_tag="PN977_002895"
     CDS             10915..11463
                     /gene="rimM"
                     /locus_tag="PN977_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HDI6042912.1"
                     /translation="MSKQLTAQAPVDPIVLGKMGSSYGIRGWLRVFSSTEDAESIFDY
                     QPWFIQKAGQWQQVQLESWKHHNQDMIIKLKGVDDRDAANLLTNCEIVVDSSQLPQLE
                     EGDYYWKDLMGCQVVTTEGYDLGKVVDMMETGSNDVLVIKANLKDAFGIKERLVPFLD
                     GQVIKKVDLTTRSIEVDWDPGF"
     gene            11494..12261
                     /gene="trmD"
                     /locus_tag="PN977_002896"
     CDS             11494..12261
                     /gene="trmD"
                     /locus_tag="PN977_002896"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HDI6042913.1"
                     /translation="MWIGIISLFPEMFRAITDYGVTGRAVKNGLLSIQSWSPRDFTHD
                     RHRTVDDRPYGGGPGMLMMVQPLRDAIHAAKAAAGEGAKVIYLSPQGRKLDQAGVSEL
                     ATNQKLILVCGRYEGIDERVIQTEIDEEWSIGDYVLSGGELPAMTLIDSVSRFIPGVL
                     GHEASATEDSFAEGLLDCPHYTRPEVLEGMEVPPVLLSGNHAEIRRWRLKQSLGRTWL
                     RRPELLENLALTEEQARLLAEFKTEHAQQQHKHDGMA"
     gene            12303..12650
                     /gene="rplS"
                     /locus_tag="PN977_002897"
     CDS             12303..12650
                     /gene="rplS"
                     /locus_tag="PN977_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012768745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HDI6042914.1"
                     /translation="MSNIIKQLEQEQMKQDVPSFRPGDTVEVKVWVVEGSKKRLQAFE
                     GVVIAIRNRGLHSAFTVRKISNGEGVERVFQTHSPVVDSISVKRRGAVRKAKLYYLRE
                     RTGKAARIKERLN"
     gene            complement(12727..13209)
                     /locus_tag="PN977_002898"
     CDS             complement(12727..13209)
                     /locus_tag="PN977_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HDI6042915.1"
                     /translation="MIKHLVAPLIFTSLILTGCQSPQGKFTPEQVAAMQSYGFTESAG
                     DWSLGLSDAILFAKNDYKLLPESQQQIQTMAAKLASTGLTHARMDGHTDNYGEDSYNE
                     VLSLKRANVVADAWAMGGQIPRSNLTTQGLGKKYPIASNKTAQGRAENRRVAVVISAP
                     "
     gene            complement(13225..14451)
                     /gene="dgcN"
                     /locus_tag="PN977_002899"
     CDS             complement(13225..14451)
                     /gene="dgcN"
                     /locus_tag="PN977_002899"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcN"
                     /protein_id="HDI6042916.1"
                     /translation="MMDNDNSLHKRPTFKRALRNISMTSIFITMMLIWLLLSVTSVLT
                     LKQYAQKNLALTAATMTYSLEAAVVFADGPAATETLAALGQQGQFSTAEVRDKQQNIL
                     ASWHYTRKDPGDTFSNFISHWLFPAPIIQPIRHNGETIGEVRLTARDSSISHFIWFSL
                     AVLTACILLASGIAITLTRHLHNGLVEALKNITDVVHDVRSNRNFSRRVSEERIAEFH
                     RFALDFNSLLDEMEEWQLRLQAKNAQLLRTALHDPLTGLANRAAFRSGINTLMNSSDA
                     RKTSALLFLDGDNFKYINDTWGHATGDRVLIEIAKRLTEFGGLRHKAYRLGGDEFAMV
                     LYDVQSESEVQQICSALTQIFNLPFDLHNGHQTTMTLSIGYAMTIEHASAENLQELAD
                     HNMYQAKHQRAEKLVR"
     gene            complement(14441..14959)
                     /locus_tag="PN977_002900"
     CDS             complement(14441..14959)
                     /locus_tag="PN977_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiR family protein"
                     /protein_id="HDI6042917.1"
                     /translation="MRFSHRLFLLLILLLTGAPILAQEPSDVAKNVRMMVSGIVSYTR
                     WPALSGPPKLCIFSSSHFSNALQENGTTSLPYLPVIIHTQQEAMISGCNGFYFGNESP
                     TFQMELTEQYPSKALLLIAEQNTECIIGSAFCLIIHNNDVRFAVNLDALSRSGVKVNP
                     DVLMLARKKNDG"
     gene            complement(15109..15474)
                     /locus_tag="PN977_002901"
     CDS             complement(15109..15474)
                     /locus_tag="PN977_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2799 domain-containing protein"
                     /protein_id="HDI6042918.1"
                     /translation="MMKKFIAPLLALLVSGCQIDPYTHAPTLTSTDWYDVGMEDAISG
                     NAIKDDDAFSDSQADRGLYLKGYAEGQKKTCQTDFTYARGLSGKSFPASCNNVENASQ
                     LHEVWQKGADENASTIRLN"
     gene            15684..16754
                     /gene="aroF"
                     /locus_tag="PN977_002902"
     CDS             15684..16754
                     /gene="aroF"
                     /locus_tag="PN977_002902"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroF"
                     /protein_id="HDI6042919.1"
                     /translation="MQKDALNNVHITDEQVLMTPEQLKAAFPLSLQQEAQIADSRKTI
                     SDIIAGRDPRLLVVCGPCSIHDPETALEYARRFKALAAEVSDSLYLVMRVYFEKPRTT
                     VGWKGLINDPHMDGSFDVEAGLQIARKLLLELVNMGLPLATEALDPNSPQYLGDLFSW
                     SAIGARTTESQTHREMASGLSMPVGFKNGTDGSLATAINAMRAAAQPHRFVGINQAGQ
                     VALLQTQGNPDGHVILRGGKAPNYSPADVAQCEKEMEQAGLRPSLMVDCSHGNSNKDY
                     RRQPAVAESVVAQIKDGNRSIIGLMIESNIHEGNQSSEQPRSEMKYGVSVTDACISWE
                     MTDALLREIHQDLNGQLTARVA"
     gene            16765..17886
                     /gene="tyrA"
                     /locus_tag="PN977_002903"
     CDS             16765..17886
                     /gene="tyrA"
                     /locus_tag="PN977_002903"
                     /EC_number="1.3.1.12"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydrogenase"
                     /protein_id="HDI6042920.1"
                     /translation="MVAELTALRDQIDEVDKALLNLLAKRLELVAEVGEVKSRFGLPI
                     YVPEREASMLASRRAEAEALGVPPDLIEDVLRRVMRESYSSENDKGFKTLCPSLRPVV
                     IVGGGGQMGRLFEKMLTLSGYQVRILEQHDWDRAADIVADAGMVIVSVPIHVTEQVIG
                     KLPPLPKDCILVDLASVKNGPLQAMLAAHDGPVLGLHPMFGPDSGSLAKQVVVWCDGR
                     KPEAYQWFLEQIQVWGARLHRISAVEHDQNMAFIQALRHFATFAYGLHLAEENVQLEQ
                     LLALSSPIYRLELAMVGRLFAQDPQLYADIIMSSERNLALIKRYYKRFGEAIELLEQG
                     DKQAFIDSFRKVEHWFGDYAQRFQSESRVLLRQANDNRQ"
     gene            complement(17929..19089)
                     /gene="pheA"
                     /locus_tag="PN977_002904"
     CDS             complement(17929..19089)
                     /gene="pheA"
                     /locus_tag="PN977_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydratase"
                     /protein_id="HDI6042921.1"
                     /translation="MTSENPLLALREKISALDEKLLALLAERRELAVEVGKAKLLSHR
                     PVRDIDRERDLLERLITLGKAHHLDAHYITRLFQLIIEDSVLTQQALLQQHLNKINPH
                     SARIAFLGPKGSYSHLAARQYAARHFEQFIESGCAKFADIFNQVETGQADYAVVPIEN
                     TSSGAINDVYDLLQHTSLSIVGEMTLTIDHCLLVSGTTDLSTINTVYSHPQPFQQCSK
                     FLNRYPHWKIEYTESTSAAMEKVAQAKSPHVAALGSEAGGTLYGLQVLERIEANQRQN
                     FTRFVVLARKAINVSDQVPAKTTLLMATGQQAGALVEALLVLRNHSLIMTRLESRPIH
                     GNPWEEMFYLDIQANLESAEMQKALKELGEITRSMKVLGCYPSENVVPVDPT"
     misc_feature    complement(19111..19236)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            complement(19188..19235)
                     /gene="pheL"
                     /locus_tag="PN977_002905"
     CDS             complement(19188..19235)
                     /gene="pheL"
                     /locus_tag="PN977_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pheA operon leader peptide PheL"
                     /protein_id="HDI6042922.1"
                     /translation="MKHTPFFFAFFFTFP"
     gene            complement(19339..19680)
                     /gene="raiA"
                     /locus_tag="PN977_002906"
     CDS             complement(19339..19680)
                     /gene="raiA"
                     /locus_tag="PN977_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HDI6042923.1"
                     /translation="MTMNITSKQMEITPAIRQHVADRLAKLEKWQTHLINPHIILSKE
                     PQGFVADATINTPNGVLVASGKHEDMYTAINELINKLERQLNKLQHKGEARRAATSVK
                     DANFVEEVEEE"
     gene            complement(19951..20688)
                     /gene="bamD"
                     /locus_tag="PN977_002907"
     CDS             complement(19951..20688)
                     /gene="bamD"
                     /locus_tag="PN977_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HDI6042924.1"
                     /translation="MTRMKYLVAAATLSLFLAGCSGSKEEVPDNPPNEIYATAQQKLQ
                     DGNWRQAITQLEALDNRYPFGPYSQQVQLDLIYAYYKNADLPLAQAAIDRFIRLNPTH
                     PNIDYVMYMRGLTNMALDDSALQGFFGVDRSDRDPQHARAAFSDFSKLVRGYPNSQYT
                     TDATKRLVFLKDRLAKYEYSVAEYYTERGAWVAVVNRVEGMLRDYPDTQATRDALPLM
                     ENAYRQMQMNAQAEKVAKIIAANSSNT"
     gene            20823..21803
                     /gene="rluD"
                     /locus_tag="PN977_002908"
     CDS             20823..21803
                     /gene="rluD"
                     /locus_tag="PN977_002908"
                     /EC_number="5.4.99.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017383977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(1911/1915/1917) synthase
                     RluD"
                     /protein_id="HDI6042925.1"
                     /translation="MAQRVQLTATVSENQLGQRLDQALAEMFPDYSRSRIKEWILDQR
                     VLVNGKVCDKPKEKVLGGEQVAINAEIEEEARFEPQDIPLDIVYEDDDIIVINKPRDL
                     VVHPGAGNPDGTVLNALLHYYPPIADVPRAGIVHRLDKDTTGLMVVAKTVPAQTRLVE
                     SLQRREITREYEAVAIGHMTAGGTVDEPISRHPTKRTHMAVHPMGKPAVTHYRIMEHF
                     RVHTRLRLRLETGRTHQIRVHMAHITHPLVGDPVYGGRPRPPKGASEAFISTLRKFDR
                     QALHATMLRLYHPISGIEMEWHAPIPQDMVELIEVMRADFEEHKDEVDWL"
     gene            21800..22531
                     /gene="pgeF"
                     /locus_tag="PN977_002909"
     CDS             21800..22531
                     /gene="pgeF"
                     /locus_tag="PN977_002909"
                     /EC_number="1.10.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphenol oxidase"
                     /protein_id="HDI6042926.1"
                     /translation="MSKLIVPQWPLPKGVAACSSTRIGGVSLPPYDSLNLGAHCGDNP
                     DHVEENRKRLFAAGNLPSKPVWLEQVHGKDVLKLTGEPYASKRADASYSNTPGTVCAV
                     MTADCLPVLFCNRAGTEVAAAHAGWRGLCAGVLEETVSCFADNPENILAWLGPAIGPR
                     AFEVGAEVREAFMAVDAEASTAFIQHGDKYLADIYQLARQRLASVGVEQIFGGDRCTY
                     TENETFFSYRRDKTTGRMASFIWLI"
     gene            22661..25234
                     /gene="clpB"
                     /locus_tag="PN977_002910"
     CDS             22661..25234
                     /gene="clpB"
                     /locus_tag="PN977_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002461515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HDI6042927.1"
                     /translation="MRLDRLTNKFQLALADAQSLALGHDNQFIEPLHLMSALLNQEGG
                     SVSPLLTSAGINAGQLRTDINQALNRLPQVEGTGGDVQPSQDLVRVLNLCDKLAQKRG
                     DNFISSELFVLAALESRGTLADILKAAGATTANITQAIEQMRGGESVNDQGAEDQRQA
                     LKKYTIDLTERAEQGKLDPVIGRDEEIRRTIQVLQRRTKNNPVLIGEPGVGKTAIVEG
                     LAQRIINGEVPEGLKGRRVLALDMGALVAGAKYRGEFEERLKGVLNDLAKQEGNVILF
                     IDELHTMVGAGKADGAMDAGNMLKPALARGELHCVGATTLDEYRQYIEKDAALERRFQ
                     KVFVAEPSVEDTIAILRGLKERYELHHHVQITDPAIVAAATLSHRYIADRQLPDKAID
                     LIDEAASSIRMQIDSKPEELDRLDRRIIQLKLEQQALMKESDEASKKRLDMLNEELSD
                     KERQYSELEEEWKAEKASLSGTQTIKAELEQAKIAIEQARRVGDLARMSELQYGKIPE
                     LEKQLEAATQLEGKTMRLLRNKVTDAEIAEVLARWTGIPVSRMMESEREKLLRMEQEL
                     HHRVIGQNEAVDAVSNAIRRSRAGLADPNRPIGSFLFLGPTGVGKTELCKALANFMFD
                     SDEAMVRIDMSEFMEKHSVSRLVGAPPGYVGYEEGGYLTEAVRRRPYSVILLDEVEKA
                     HPDVFNILLQVLDDGRLTDGQGRTVDFRNTVVIMTSNLGSDLIQERFGELDYAHMKEL
                     VLGVVSHNFRPEFINRIDEVVVFHPLGEQHIASIAQIQLKRLYKRLEERGYEIHISDE
                     ALKLLSENGYDPVYGARPLKRAIQQQIENPLAQQILSGELVPGKVIRLEVNEDRIVAV
                     Q"
BASE COUNT         6318 a         5980 c         6684 g         6479 t
ORIGIN      
        1 caggcaggta ttaaagcctc attcagcagt tcgttaagcg ggaatctcgg ggtggggtat
       61 agccatggtg ccggtgtgga atccccgtgg aacgcagtgg ctggtgtgaa ctggtcgttc
      121 tgaccatcaa cgaaaaagcc cacatctgtg ggctttcata tcaccaggag ccgcggctcc
      181 tttgcgtatc cttttttgtc tcctcaccgt ctggtcggtg tcctgctgag actgctaact
      241 tcctgttttt attggtgttg tccttacacc gtccaatcat gattggtgga gctggcggga
      301 gttgaacccg cgtccgaaat tcctacatcc tcggtactac atgcttagtc agtctttaca
      361 ttcgcttgcc agctgcggac ggacacgcca ctaacaaact agcctgatta agttttaacg
      421 cttcaacccc aggcagggct tccacgcgat ctcttttggg tttgacctct cttgatcccc
      481 gtcctaagag cggaggctag ggagagaggg ctctaagcag gttattaagc tgctaaagcg
      541 tagttttcgt cgtttgcgac tattttttgc ggctttttac gaggccaacc gcccctcggc
      601 atgcaccttg ggtttcgcaa atcccgtcga atccagaatc agccccaatg tgtgacggta
      661 agtataccag attcatgagt gccatgacca gcctcaatgg cgttatcatt aaagatttag
      721 cacccatgta gcctgatttt tattcgatta agcaatggga tggcaacatt tgtgtcggaa
      781 gtgatagcca ataagatgtt cattcgcgcc gccggagagg gaggcgcggt gaggaactgg
      841 tcaataattg cagtgcaggt ttaacggtgg gcgtttttca tgatacgcgc tttatccacc
      901 tgccattcgc gttctttgat atctgaacgt ttatcatgct gtttcttacc tttggcgacg
      961 ccgattttta ctttgcacca ggcatttttc cagtacaggg agagcgccac tacggtatag
     1021 ccttctcgat tgacgcgacc gtacaatgag tccagttcgc gctggttgag aagtaacttg
     1081 cgggtacggg taggatcgca caccacatgc gtggaggcca cagccattgg cgtgatgtta
     1141 gcgccaaaca gaaatgcctc tccgtcacgc agaaggacgt agctgtcgct gatattggct
     1201 tttcctgcgc gcagggattt aacctcccag ccttgcaggg caagtcccgc ctcgaactct
     1261 tcttcgataa agtattcgtg acgggcgcgc ttgttaagcg cgatggtcgc tgaaccaggt
     1321 ttatgtgctt ttttcttcgt cataagcgtc gtgaatcatc ggtaatctga aatcgaaaac
     1381 accccatatc aatcctgcgg gggacaaggc tctatcttag catgaacccg atgttaccca
     1441 gcgccgggat agcgtttttt ttacagcagg ataaatgata ttatttgttg gatttttgtt
     1501 gatggaaatt gttatgcctc agattagccg gaccgcactg gtaccctaca gcgcggagca
     1561 aatgtatcag ttagtgaatg acgttcagtc ttatcctcag tttttgccgg gttgtaccgg
     1621 aagtcggatt ctggagtcca ctcctgggca gatgactgcg gcggtaaatg tctctaaggc
     1681 tgggatcagc aaaacgttta ctacccgcaa ccagttgacc agtaaccaaa gtattcttat
     1741 gaatctggtg gatgggccgt tcaagaaatt gatcggtgga tggaagttta cgccgctgag
     1801 ccaggaggcg tgtcgtatcg agtttcatct cgactttgag tttaccaata agttgattga
     1861 actcgccttt ggtcgcgtgt ttaaagagct ggcggctaat atggtccagg cttttacggt
     1921 tcgtgcgaaa gaggtttaca gtgccaggta aaattgccgt tgaggtggct tatgcgctac
     1981 ctgagaagca gtacctgcag cgtgtgacgc tgcaggaggg tgcgacggtt gaagaagcta
     2041 ttcgcgccag tggcttgctg gaattgcgta ccgatatcga tttaactaaa aacaaagtcg
     2101 gcatttacag ccgtccggcc aaactgagcg atacggtgca tgatggcgat cgggtggaga
     2161 tttatcgtcc tctcattgcc gatccgaaag agcttcgcag gcaacgagca gaaaaatcag
     2221 cgaataaata acagacagaa aaaaggtgct cattgagcac cttttttaac ctctttgaga
     2281 gcaactttat tattagttac cactcagcgc aggtttgtta tcaatgttgg ccaacacacc
     2341 gctactgtta aaggtcagcg tcagcgtttg ctgcgttaca ccttcatgac caggttgctg
     2401 gcggaagaca tagaaccagg tattcgtacc aaatggatcg gacatcagcg gtgtacccaa
     2461 tgcgtacgca acttgttgtt gcgtcatgcc aacacgtatt ttggatacgt cgttagcggt
     2521 cagataattc ccctggttga tgtcaggacg gtaaaccact cgctccagag tggaacagcc
     2581 tgcggtcaac atcaatagta ctgctgctgc agcagtcagc gttttacagc gcatagtgat
     2641 ttgattcctt ttcgggcccg agcagtacgt ggctcatctg taatatgccg atgataatag
     2701 acctttcatc actttaaaac cttttgcttt ccggtcctac ggcgttttgc tgtttactct
     2761 gaccgtgaag caggaaaaaa gtttacgccg caagcagttc tttcgcattc gccagtgtgt
     2821 tacgtgtgac ttcactgcca ccaagcaggc gcgccagctc ttgtaaccgc gcttttttgt
     2881 ccagcgactc catatgcgtt tctgtcatcg caccatcggt ttctttgctg acaaaatagt
     2941 gttgatgacc acatcccgcg acttgtggca ggtgggtaac acacatcacc tgagttgatt
     3001 cgccgagttg acgcagcagt ttgccaacaa ctgccgctgt tgggccgcta atccctacat
     3061 ccacttcatc aaaaatcagt gccggggttt ccattttacg cgccgtgatg acctgaattg
     3121 ccaatgcgat gcgtgacaat tcaccaccgg atgcgacttt ggcaataggc tgcattggct
     3181 gacctgggtt ggtggttacc cgaaattcaa tacgatcagc gccgtcagcg cccaggtgat
     3241 gctcgtcaaa tttaacatca atcgtaaact gcccatgcgg catagagagt gcatgcatac
     3301 tgtcggtgat cagctgtgca agttcttctg catagtgctg gcgttgctgg tgtaatgcgt
     3361 tcgcggtttc cagtgcctgc tgatgatgtt tcgttaccgc cagcgcgagc gtttcttgtg
     3421 agtcggcctg atcgtccagt tgctgctgtt cttccagtag cgactggtaa tactgtggca
     3481 atgcctcagg gctgacgtga tgtttacgtg ccagcgaaat ctgttttgag atgcgctgtt
     3541 caagttcaaa cagtcggtta ggatcgaggt ccagacgatc gcagtagtgg cgcagttcat
     3601 cgctggcttc agcaatctgg atggtagctt cttccagcat atcaagtacg ccggacagtt
     3661 tgctgtccat gccaatcaat tcgctcacca gttgtttagc cgtgtaaagc tgactttgca
     3721 ggtttgcgtc ttcaccgtcg gccattaatg ccaatgcatt ctggctggtg gtcagcaatt
     3781 gaccgctgtt cgccagacgt ttgtactctt cgtcgatttg ctcaaactct ccgggctgcg
     3841 gattaaattc gttaagttct tttaattggt attgcagcag ttctgcacgg gcggcgcgtt
     3901 cctgacttaa ctgttgatga tgcgcgaggt cacggcagct ttgatgccac aactgataac
     3961 gtgcggtcat ttcctggagt agagaggttt cattggcata gccatcaagc aggaattttt
     4021 ggtgctcggg tttggtgagt aactgatgag cgtgctgacc atggatctga atcagcaact
     4081 gacccagttc gcgcagttgt gaaagaggaa ccgctgtacc gttgataaaa ccacgggagc
     4141 gaccatcgct gctgattacg cgacgaagca aacattcatg cccgtcttca agctggtttt
     4201 cttccagcca gcgcagggcc gctggcgtat ctttcagaga aaaacgggcg cacaggtcag
     4261 cgcgagcagc gccggtacgc accatgtcgg cttcagcgcg accaccgaga caaagaccga
     4321 gggcatctat tgcaatagat ttacccgcgc cggtctcgcc agttattacg gtcatgccgc
     4381 tatgaaaatc aatctcaagc tcacgaacga tagcaaagtt gctgatggtc agttgtgcca
     4441 acatagttgt tttcctgtat gaaaaaccat tactgttatt gtgtacagta taaactggtt
     4501 ttatatacag taaagaggct ggcgtgaaat tagaataatt tttttgacca gccgagcttg
     4561 gtgcttaatg tattgaaata actgtaatct ttcggatgaa tcagattcag atggtaatca
     4621 cagcgacgaa tcaggacatc ttcaccttcc tgaatcggca gtgctatctg gctgtcgcaa
     4681 ctgatttcca ggtcgttacg gcgatgcgaa aaacgcagac ggatcgtgct gctgctgttt
     4741 atgaccagtg gtcgtgctga caacgtatgc gggaacatgg gcaccagggt aatcgcatcc
     4801 agagagggtg tgagaatagg accgcccgca gagagggaat aggcggtgga gcctgttggc
     4861 gtcgaaataa tcagtccatc ggaacgctga gaaaacgcaa agatctcgtc gatatacact
     4921 tcgaactcaa tcatatgcgc cactttgcca ggatgaagta ccacttcgtt aatcgcggtg
     4981 ctgatgcgtt tctggcaatc ttgctgacaa acttgcgctt ccagcaaaaa acgtttctcg
     5041 ctgatgtagt ggccttccag cacatcggct aattgttgct gggcgttatc ggggtcaagg
     5101 tcagtcagga aacccaggtt gccacggttg attccaataa ctttaatatc gtaacgggcg
     5161 agtgtgcgcg ccgcgcccag catattaccg tcgccaccaa caactaccgc gagatctgcc
     5221 agttgcccaa tctccgcgag cgtgccagtt ttcacattct tcagttgcag ttcgtgagcg
     5281 atttgttgct caacgatgac ctcgtaacct tttgcgcaca gccagcggta gagcatttca
     5341 tgtgttgtca gtgcagtggg gtgccgtggg tgtcccacaa tgccaataca cttgaaatga
     5401 ttattcattt ttccgaggtc cttgttgcga agattgatga caatgtgagt gcttcccttg
     5461 aaaccctgaa actgatcccc ataataagcg aagttagcga gatgaatgcg aaaaaaacgc
     5521 ggagaaattc atgagtagta aagaacagaa aacgcctgag gggcaagccc cggaagaaat
     5581 tatcatggat cagcacgaag agattgaggc agttgagcca gaagcttctg ctgagcaggt
     5641 ggatccgcgc gatgaaaaaa ttgcgaatct cgaagctcag ctggctgaag cccagacccg
     5701 tgaacgtgac ggcattttgc gcgtaaaagc cgaaatggaa aacctgcgtc gtcgtactga
     5761 actggatatt gaaaaagctc acaaattcgc gctggagaaa ttcatcaacg aattgctgcc
     5821 ggtaatcgat agtctggatc gcgcgctgga agtggctgat aaagctaacc cggatatgtc
     5881 cgcgatggtt gaaggcattg agttgacgct gaagtcgatg ctggatgtgg tgcgtaaatt
     5941 tggcgttgaa gttatctctg aaaccaacgt gccgctggac ccgaatgtgc atcaggccat
     6001 cgcaatggtg gaatctgacg acgtagcgcc gggtaacgta ctgggcatta tgcagaaggg
     6061 ttatacgctg aacggtcgta cgattcgtgc ggcgatggtt actgtagcga aggcgaagta
     6121 aatatctgat ggccgacgcg aagtcggcca tttttttagc ttaaaaaatc ctgtacttcc
     6181 agccacggcg tgttgaaatc atctattttt ttatattcgt tcattggcag aacgaccacg
     6241 ttataggtgt cagatccttc attcagatca aaaatctgaa agcccctttt attgagttcg
     6301 gtttggatgc gttcaaagat ttcgtcaagg taacggtctc cggcttcaca gacatcggag
     6361 atttcatcct cttgggttat ttcgacaaga agactcctta attcctcaga agctgtcagg
     6421 ttttttgtga gctcataaaa gcgatctgca gcaaaattag ccagctcacc ttcttcatct
     6481 tcacctttcc agtcaacgag ttgaattagt ccttcaccgc gcagaacctg taaaaatgcc
     6541 tgcgccaggt catgatttcc gagatcgcct ccactgcaat cgagaacgtc ttgtaatcca
     6601 tattcgtctt catcttcgtc ttcaaagatt tcaggttgcg tcgaaagttg agagaaaata
     6661 gtgggccata aggttggggt gtgctgtaaa aaaacgctgg cgacgacgtt gagatcgtct
     6721 gcaaaatcct tatcccattc aattaaataa tcttctgaca taacatcaat atcctttccg
     6781 aatgtttgaa aagacattat tacattgcta agtaaatgac aggaattgat ggaatcctaa
     6841 aagacggcga taaaaaaaaa ggcgaaccgg agttcgccat tgcgttactc cgccacgctc
     6901 tcgcgtagcg gtttcacagg aaaaactttt acctgcttaa tcatattgtc ctgaacgtcg
     6961 agaatatcaa tatcgtactc gccaatacgc acgcgggtgc ctgctaccgg gatctcctcc
     7021 agcgcttcca gaatgacgcc gttaacagta cgagcatcat cttccggtag atgccagtta
     7081 aaggctttgt taatttctcg cacgttggca gtgccatcaa taatcaccga accgtcgttt
     7141 tgtggcgtaa cttcttcggc aagtgttggc gacatcgacg tggtgaaatc gccgacaatc
     7201 tcttccagaa tatcttcaac cgtcaccagc ccctgaatgt ctccatactc gttgacgacc
     7261 aggccgactt tctttttgtt gcgctgaaac ttcaccaact gcgtgctgag cggcgtacct
     7321 tccgggacaa aatagatctc gtccgcggcg cgtagcatgg tttctttggt gaactctttt
     7381 ttctccgaca tgagtcgcca ggcttcacgc acacgcagca tactgatggc gtcgtccagc
     7441 gaatcacggt agagcacgat gcgcccgtga ggtgagtggg agagttggcg cagaatcgat
     7501 ttccagtcat cgttgatatc aataccgata atttcactgc gcggcaccat gatgtcatca
     7561 acggtcattt tttccagatc gagcaccgac agcagcatat cctgattgcg acgggaaatt
     7621 tgtgagcgcg attcgtgcac gatagtgcgc aactcttctt tgctcaaaga gccgctaacc
     7681 acgatatcgg ttttgatacc catcatgcgc atcagcatac gggtgatagc attcagcaac
     7741 cagaccagcg gcatcatcaa aatttgcagc ggagccagca gaaaactact cggataagcg
     7801 actttttccg ggtacagcgc ggcaatggtt ttcggcaata cctcagcaaa aaccagcacg
     7861 acaaaagtca gcacaccagt cgcaattgcc acgcccgcat cgccgtacaa acgcatccca
     7921 acaatagtgc ctagcgcgga ggcaagaata ttgaccaggt tattgccgat taacaccagg
     7981 ctaatcaggc ggtctggctt acgcagcaat ttttcgacgc gtttggccga gcgattaccc
     8041 tgtttcgcca tatgtcgcag acgatagcgg ttgagggtca tcattccggt ttcggacccg
     8101 gaaaaatagg ctgaaatgac caccatgatg atcagaataa tgatcaacgt agtagtagaa
     8161 atgtgttcca ggggaaactc cttttctggg tttagctgat taactgctgg acaattcggc
     8221 tgccgaagta ggccagtgtc agaatgactg cacccgcaac gttaaaccag acgacgcggc
     8281 gtccacgcca tccttcatga taatgtcccc acagcagcac aatatagaca aaccacgcca
     8341 cgatagagag cacggccttg tcgatatttt ccatgctaaa taagttgtgc atgtagaaca
     8401 ggccagtgca aagcgtgagc gttagcagca ccacgccaat ctgcgtgatg tggaacattt
     8461 tacgctcgat actcatcaat ggcggcattt cctggttaaa cgccagcttc ttgttcttca
     8521 gttggtaatc aatccacgcc agttgcagcg cgtacagggc ggcgataatc agcgtggcat
     8581 aggaaaagag cgataagcca atgtgcacca gcatcccagg cgttgcttcc agatgggtga
     8641 tgtattcatt gggcatgaag gtcgccagcg ccaggttgat aagcgcaaag gcatagacaa
     8701 tgggtagcag caaccagcca cgattgcgag aagccacaat ggtcattacc gtacagatca
     8761 tcaaactgac caatgaacca acgttcagca ggctgaggtt ttgtccgcta tcaccgtcgg
     8821 gcaggatgcg ggcttccaga gcgattgcgt ggcagaccag cgcaatgacc gcagaaataa
     8881 tagccatgcg ccgccagccg ccgttttttt gcagcagacc gggaacaatc agcgcaagac
     8941 tgacggagta ggcgacaagc gcgagcagag caaaaacggg catagtaatg tcgacagttt
     9001 gagcaataag aaagagaagc agtatagcgt caggtgaacg ctgctccaac cgttgcataa
     9061 caacaaagac gccttcatgt tatactgcgg caaaatactg atgatgtgtc gcgattgcgg
     9121 ccaaccgttt ccaccccagg cgagagacaa tgtttgataa tttaaccgat cgtttgtcgc
     9181 gcacgctgcg caatatcagt ggccgtggac gcctcactga agacaacgta aaagatacgc
     9241 tgcgcgaagt gcgcatggcg ctgctggagg cggacgtcgc tctgccggta gtgcgtgagt
     9301 ttatcaatcg cgtaaaagag aaagcggttg gtcatgaagt taataagagc ctgacgccgg
     9361 ggcaggagtt cgtcaaaatc gtccgtaacg aactggttgc ggcgatgggc gaagagaacc
     9421 agaccctgaa tctggctgcg caaccgccag cggtggtact gatggcgggc ctgcaaggtg
     9481 ccggtaaaac aaccagcgtg ggcaagctcg gtaagttcct gcgcgagaag cacaagaaaa
     9541 aagtgttggt ggtttctgcc gacgtttatc gtccggcggc aatcaaacag ctggagacgc
     9601 tggcagagca ggtgggcgtt gatttcttcc cttctgatgt tggtcagaag ccggtagata
     9661 tcgttaacgc ggcgctgaaa gaagccaaac tgaaattcta cgacgtgctg ctggtggata
     9721 ccgccggtcg tctgcacgtt gacgaagcga tgatggacga gatcaaacaa gtccatgcgt
     9781 cgattaatcc ggttgaaacc ctgtttgtgg ttgacgccat gaccggtcag gatgcggcca
     9841 atacggcaaa agcatttaat gaagcgttat cgcttaccgg cgtagtgttg accaaagtgg
     9901 acggcgatgc ccgcggcggt gcggcgctgt ctattcgtca catcactggc aaaccgatca
     9961 agttcctcgg tgttggcgag aaaactgagg cgctggagcc gttccatccg gaccgcatcg
    10021 cgtcgcgcat tctcggcatg ggcgacgtac tgtcactgat cgaagatatt gaaagcaaag
    10081 ttgaccgcgc gcaggcagag aaattagcca gcaagctgaa aaaaggcgac ggcttcgatc
    10141 tcaacgattt tcttgagcaa ctgcgccaga tgaaaaatat gggcggcatg gccagcctga
    10201 tgggcaaact gccgggcatg gggcagatcc cggataacgt caagtcgcag atggacgata
    10261 aagtgctggt gcgtatggaa gccatcatca actcgatgac gatgaaagag cgcgctaagc
    10321 cagaaatcat caaaggttcg cgtaaacgcc gtatcgctgc cggttgcggt atgcaggtgc
    10381 aggacgttaa ccgtcttctg aaacagttcg acgacatgca gcgcatgatg aagaaaatga
    10441 agaagggcgg aatggcaaag atgatgagaa gcatgaaagg gatgatgccg ccgggatttc
    10501 ctggtcgcta aatgacctta tgcgacagag ttattaactg ctgattgcat tttctccaga
    10561 aatcagtaaa attttcgggc ttttaatatg acaccggact ccgttcctcg atggggtccg
    10621 gttgttttat tcacacaaga ggatgttatg gtaactattc gtttagcacg tcacggcgct
    10681 aaaaagcgtc cgttctacca ggttgttgtc gctgacagcc gtaatgcacg caacggtcgc
    10741 ttcatcgagc gcgttggttt cttcaaccca atcgctagcg aaaaagaaga aggcactcgc
    10801 ctggatctgg atcgcatcgc tcactgggtt ggccagggcg caactatttc tgatcgcgtt
    10861 gctgcgctga tcaaagaagt aaacaaagca gcttaatctg tcacggtggt catgatgagc
    10921 aaacaactca ccgcgcaagc acctgttgat cccatcgttt tgggaaaaat gggttcgtct
    10981 tacggtattc gtgggtggct cagagtgttt tcttccaccg aagacgccga aagcattttt
    11041 gactatcagc cctggtttat ccagaaggcg ggtcagtggc agcaagtcca gctggaaagc
    11101 tggaagcacc acaatcagga catgatcatc aagctgaaag gcgttgacga tcgtgatgcg
    11161 gcgaacctgc tgacgaattg tgaaattgtc gtggattcat cgcagttgcc tcagcttgaa
    11221 gagggtgact actactggaa agacctgatg ggctgccagg tagtaaccac tgaaggctac
    11281 gatctcggta aagtcgtcga tatgatggaa accggatcta atgacgttct cgtcattaag
    11341 gcaaacctga aagatgcgtt tggtatcaag gaacgtctcg taccgttcct cgatgggcag
    11401 gttatcaaga aagtcgatct cactactcgt tcaatcgaag tagattggga tcctggtttt
    11461 taaaccaccg gataaacggt aaaagacggc gctatgtgga ttggcataat tagcctgttt
    11521 cctgaaatgt tccgcgcaat taccgattac ggggtaactg gccgggcagt taaaaatggc
    11581 ctgctgagca tccagagctg gagtcctcgc gacttcacgc atgaccggca ccgtaccgtg
    11641 gacgatcgtc cttacggcgg cggaccgggg atgttaatga tggtgcaacc cttgcgggac
    11701 gccattcatg cagcaaaagc cgcggcgggt gaaggcgcaa aggtgattta tctgtcacca
    11761 cagggacgca agcttgatca agcgggcgtc agcgagctgg caacgaatca aaaattgatt
    11821 ctggtgtgcg gtcgctacga aggtatagat gagcgcgtga tccaaaccga aattgatgaa
    11881 gaatggtcaa tcggcgatta cgttctcagt ggtggtgagt taccagcaat gacgctgatt
    11941 gactccgttt cccggtttat tccgggagta ctgggacatg aggcctcggc aacggaagat
    12001 tcctttgctg aaggattgct ggattgcccg cactatacgc ggcctgaggt gttagaaggg
    12061 atggaagttc cgccagtgtt actgtcgggc aaccatgccg agatacgtcg ctggcgtttg
    12121 aaacagtcgc tgggccgtac ctggcttaga agacctgaac ttctggaaaa cctggctctg
    12181 actgaagagc aagcaaggtt gctggcggag ttcaaaacgg aacacgcaca acagcaacat
    12241 aaacatgatg ggatggcgta agcccccgag atatcagttt acccaggata agagattaaa
    12301 ttatgagcaa cattattaag caacttgaac aagagcagat gaagcaggac gtaccttcct
    12361 tccgtccggg tgataccgtg gaagtgaaag tatgggttgt tgaaggttcc aaaaaacgtc
    12421 tgcaggcatt cgagggcgtg gttatcgcta ttcgtaaccg cggtctgcac tctgcattca
    12481 ctgttcgtaa aatttccaac ggcgaaggcg ttgagcgtgt cttccagact cactctccgg
    12541 tagttgacag catttctgtc aaacgtcgtg gtgctgttcg taaagctaaa ctgtactacc
    12601 tgcgtgagcg tactggtaag gctgctcgta tcaaagagcg tcttaactaa gatttcgctt
    12661 aagcgacatc ctgttaagaa gggctggcca attggctggc ccttttttta tctatcaaaa
    12721 ttgcgtttac ggtgcactaa tcaccacagc gacgcggcgg ttctcggcgc ggccctgggc
    12781 ggtcttgtta ctggctatgg gatatttttt tcctaaaccc tgtgtggtga gattgctgcg
    12841 cggaatttgt ccacccatag cccatgcatc ggccacgaca ttcgcccgtt ttaatgataa
    12901 gacttcgttg taactgtctt caccatagtt gtcggtgtgt ccatccatac gggcatgtgt
    12961 tagccctgtc gaggccaatt tggctgccat ggtttggatt tgttgctggc tttccgggag
    13021 caatttgtag tcattttttg cgaacagaat ggcatctgat aagcccagcg accagtcgcc
    13081 ggcggattca gtaaatccat aagattgcat agcggcgact tgctcaggag taaactttcc
    13141 ctgaggggac tggcagccag taagtattag cgaggtgaaa atcaggggtg ctaccaggtg
    13201 ctttatcata tcgatatatc cttgttatct caccagcttt tcggcacgct ggtgtttggc
    13261 ctgatacata ttgtgatcgg caagctcttg taaattttcc gcagaggcgt gctcaatggt
    13321 catcgcgtaa ccaatgctta atgtcatggt agtctgatgg ccattatgaa gatcaaacgg
    13381 gagattaaaa atttgtgtca gtgctgagca tatctgctgc acttcagatt ccgattgtac
    13441 atcatagagc accatagcga attcatcgcc gcccaggcgg tatgctttat gtcgcagccc
    13501 gccaaattca gttaaccgtt ttgctatttc aatcaagact ctatcgcccg tcgcatgacc
    13561 ccaggtatca ttgatatatt tgaaattatc gccatcaaga aatagtaacg ccgacgtttt
    13621 tcgggcatcg gaactgttca tcaacgtgtt gataccgcta cgaaacgctg cgcggttagc
    13681 cagcccggtt aatgggtcat gtagtgcggt acgtagaagc tgcgcatttt tggcctgtaa
    13741 acgaagctgc cactcttcca tttcatccag cagactgttg aagtcgagag cgaagcggtg
    13801 aaactctgcg atacgttctt ccgaaactcg tcgggaaaaa ttgcggttgg aacgcacatc
    13861 atgtacgaca tcggtgatgt tttttaacgc ttcaaccaga ccattgtgca aatggcgggt
    13921 gagggtaatt gcgatgcctg acgctagcag aatgcaggcg gtcaggacag cgagcgaaaa
    13981 ccagatgaaa tggctgattg aactgtcgcg agcggttaag cgaacttcgc caatggtttc
    14041 accattgtga cgaatcggct gaatgatggg ggcagggaag agccagtggc ttatgaaatt
    14101 actgaaagta tcgcctggat ccttacgggt gtaatgccag gatgccagaa tattttgctg
    14161 cttatcacgc acttctgcag ttgaaaattg gccttgctgc cccagcgctg ccagtgtttc
    14221 agttgctgca gggccatcgg caaaaacgac cgctgcttcc agactgtaag tcattgttgc
    14281 tgctgtcagt gccaggtttt tttgcgcgta ctgtttcagg gtcagcaccg aggtcacgga
    14341 aagcagcaac cagatcagca tcatagtgat aaatatgctg gtcatactga tgttgcgtaa
    14401 tgctctttta aacgtggggc gcttatgaag agaattatcg ttatccatca tttttcttcc
    14461 gtgcgagcat taagacatcc gggttgacct ttacaccgct acgcgataag gcatccaggt
    14521 ttacggcaaa tctgacgtca ttattgtgga tgattagaca aaaggcgcta ccaataatgc
    14581 actcggtatt ttgttcggca attaataata acgcctttga cggatattgt tcggttaatt
    14641 ccatctggaa tgtcggtgac tcgttgccaa aataaaaacc attgcagcct gaaatcatcg
    14701 cctcttgttg agtgtgaatg atgacaggta gataaggcaa agatgtcgtg ccattctctt
    14761 gtagggcgtt actaaaatgt gaagatgaaa agatacataa ctttggtggc cctgatagcg
    14821 caggccagcg ggtgtaactg acaataccgg aaaccatcat acgcacgttt ttcgcaacgt
    14881 cagaaggttc ctgagcgagt attggtgctc ccgtcagcag gagaatcagc agtaagaaca
    14941 gtcggtgaga aaaacgcatt cagggatcct caccatcttc gctcgaaatg catcgatatt
    15001 ttttcagaat gctcattaaa atatcattct ttcatgcggt cgtcatcaat gtgagaaata
    15061 aaacacaatg agtagaatct catgtttaat ttatttgtgg gggtttaatt aatttaatcg
    15121 tattgtgctg gcattttcat cagccccttt ttgccatact tcatgcagtt ggctggcatt
    15181 ttcaacattg ttgcagctgg caggaaagct tttaccggaa agtcctcggg cataagtaaa
    15241 atcggtctgg caagttttct tttgtccttc ggcatatcct ttaaggtata gaccgcgatc
    15301 cgcctgtgaa tcgctaaatg catcgtcatc ttttatggcg ttgcccgata tcgcatcttc
    15361 cataccaaca tcataccagt cggtgctggt caaggttggc gcgtgagtat aaggatcaat
    15421 ctgacatccg ctaaccagta aagccaacaa gggggcgata aactttttca tcatttcttt
    15481 ctcctttttc aaagcatagc ggattgtttt caaaggtggt gtaaatttat ttatacataa
    15541 acaagggtta aaagcgcgaa taaattgctt gtgtaaagaa aaatgtacga aatatggatt
    15601 gaaaacttta ctttatgtgt tatcgttacg tcatcctcgc tgaggatcaa ctatcgcaaa
    15661 cgagcataaa caggatcgcc atcatgcaaa aagacgcgct gaataacgta catattaccg
    15721 acgaacaagt tttaatgact ccggaacaac tgaaggccgc ttttccatta agcctgcaac
    15781 aggaagccca gattgctgac tcgcgtaaaa ccatttcaga tattatcgcc gggcgcgatc
    15841 ctcgtctgtt ggtagtatgt ggtccttgtt ccattcatga tccggaaact gctctggaat
    15901 atgctcgtcg atttaaagcc cttgccgcag aggtcagcga tagcctctat ctggtaatgc
    15961 gcgtctattt tgaaaaaccc cgtaccactg tcggttggaa agggttgatt aacgatcccc
    16021 atatggatgg ctcttttgat gtagaagccg ggctgcagat cgcgcgtaaa ttgctgcttg
    16081 agctggtgaa tatgggactg ccactggcga cggaagcgtt agatccgaat agcccgcaat
    16141 acctgggcga tctgtttagc tggtcagcaa ttggtgctcg tacaacggaa tcgcaaacac
    16201 accgtgaaat ggcctccggg ctttccatgc cggttggttt taaaaacggc accgacggca
    16261 gccttgcaac ggctattaac gctatgcgtg ccgccgccca gccgcaccgt tttgttggca
    16321 ttaaccaggc agggcaggtg gcgttgctac aaactcaggg gaatccggac gggcatgtga
    16381 tcctgcgcgg tggtaaagcg ccgaactata gccctgcgga tgttgcgcaa tgtgaaaaag
    16441 agatggaaca ggcgggactg cgcccgtctc tgatggtaga ttgcagccac ggtaattcca
    16501 ataaagatta tcgccgtcag cctgcggtgg cagaatccgt ggttgctcaa atcaaagatg
    16561 gcaatcgctc aattattggt ctgatgattg aaagtaatat ccacgagggc aatcagtctt
    16621 ccgagcaacc gcgcagtgaa atgaaatacg gtgtatccgt caccgatgcc tgcattagtt
    16681 gggaaatgac cgatgccttg ctgcgtgaaa ttcatcagga tctgaacggg cagctgacgg
    16741 ctcgcgtggc ttaagaggtt tattatggtt gctgaattga ccgcattacg cgatcaaatt
    16801 gatgaagtcg ataaagcgct gctgaattta ttagcgaagc gtctggaact ggttgctgaa
    16861 gttggcgagg tgaaaagccg ctttggactg cctatttatg ttccggagcg cgaggcatct
    16921 atgctggcct cgcggcgtgc agaggcggaa gctctgggtg taccgccaga tctgattgag
    16981 gatgttttgc gtcgggtgat gcgtgaatct tactccagtg aaaacgacaa aggatttaaa
    17041 acgctttgtc cgtcactgcg tccggtggtt atcgtcggcg gtggcggtca gatgggacgt
    17101 ctgttcgaga agatgctgac actatcgggt tatcaggtgc ggattctgga gcaacatgac
    17161 tgggatcgcg cggctgatat tgttgccgat gccggaatgg tgattgttag tgtgccaatc
    17221 cacgttactg agcaagttat cggcaaatta ccgcctttac cgaaagattg tattctggtc
    17281 gatctggcat cggtgaaaaa tggaccatta caggccatgc tggcggcgca tgatggtccg
    17341 gtgctggggt tacacccgat gttcggcccg gacagcggta gcctggcaaa gcaagttgtg
    17401 gtctggtgtg atggacgtaa gccggaagca taccaatggt ttctggagca aattcaggtc
    17461 tggggcgctc ggctgcatcg tattagcgct gtcgagcacg atcagaatat ggcgtttatt
    17521 caggctctgc gccactttgc tacttttgct tatgggctgc acctggcaga agaaaatgtt
    17581 cagcttgagc aacttctggc gctttcttcg ccgatttacc gccttgagct ggcgatggtt
    17641 gggcgactgt ttgcccagga tccgcaactg tatgccgaca ttattatgtc gtcagagcgt
    17701 aatctggcgt taatcaaacg ttactataag cgtttcggcg aggcgattga gttgctggag
    17761 cagggcgata agcaggcgtt tattgacagt ttccgcaagg tggagcactg gttcggcgat
    17821 tacgcacagc gttttcagag tgaaagccgc gtgttattgc gtcaggcgaa tgacaatcgc
    17881 cagtaataat ccagtgccgg atgattcaca tcatccggca ctttttcatc aggttggatc
    17941 aacaggcact acgttctcac ttgggtaaca gcccaatacc ttcatcgaac gggtgatttc
    18001 ccctaactct ttcaatgctt tttgcatttc cgctgattca agattggcct gaatatccag
    18061 ataaaacatc tcttcccacg gattaccgtg aatcggacgt gattccagac gggtcataat
    18121 tagactgtgg ttgcgcagta ccagcaacgc ttcaaccagt gcaccagctt gttgtccggt
    18181 cgccattaac aacgtcgttt tcgccggaac ctggtcagaa acgttaatgg ctttacgtgc
    18241 caacaccaca aatcgggtga agttttgtcg ctgattcgct tcaatacgct ccagtacctg
    18301 caaaccgtac aaagtgccgc cagcttcgct tcccaacgca gcaacatgcg gtgattttgc
    18361 ctgtgcaacc ttttccattg ccgcagacgt actttcggta tattcaatct tccagtgcgg
    18421 ataacgatta aggaatttgc tgcattgctg gaatggctgc ggatggctgt agaccgtatt
    18481 aatggtggat aaatcagtag tgccggagac caacaaacaa tgatcgatag ttaacgtcat
    18541 ctcgccaaca atcgacaaac tggtatgttg cagcagatcg taaacgtcgt ttatggcacc
    18601 ggagctggta ttttcaatcg gtacgacggc atagtcggcc tggccggttt ccacctgatt
    18661 aaaaatatcg gcaaatttgg cgcagccact ttcaatgaat tgctcaaagt gacgcgcagc
    18721 gtactgacga gcggcaagat gtgaatagga gcctttgggg ccgagaaaag cgatgcgtgc
    18781 tgagtgcgga ttaattttat tgagatgttg ctggagcaaa gcctgctgag ttaatacgga
    18841 atcttcaatg atgagctgga acaggcgagt aatgtaatgg gcgtccagat ggtgcgcttt
    18901 accgagcgta attaatcttt ccagcaaatc gcgttcacga tcaatatcac gtaccgggcg
    18961 atgcgagagt agtttggctt ttcccacctc gacggccagt tcgcgtcgct ctgccagtaa
    19021 tgctaataat ttttcatcca gcgcgctgat tttctctcgc agcgccagta acgggttttc
    19081 cgatgtcata gtgttgcctt tttgttatca ataaaaaagg ccccccgatt tgggaggcct
    19141 tattgttcgt cttcgcattc tgtttcacac gacgaaacgc ctcccattca ggggaaggta
    19201 aaaaagaatg cgaagaaaaa cggtgtgtgt ttcatgtttg tttccttaag tgactttagt
    19261 acagtacccg tactgttttc acgctgtcaa caaaaaacgc gcccgaaggc gcgttggcga
    19321 tacactcaat ataaaggact actcttcttc aacttcttcg acgaagttgg cgtctttcac
    19381 cgatgttgcg gcacgacgtg cttcgccttt gtgctgcagt ttattgagct gccgttccag
    19441 cttgttgatc aattcgttaa ttgcggtgta catatcttca tgtttaccac tggcaaccag
    19501 aacgccgtta ggtgtattga ttgtggcgtc agcaacaaac ccttgtggct ctttggacag
    19561 aatgatatgt ggattaatca gatgtgtttg ccatttttcc agtttggcga gacggtctgc
    19621 gacatgttgg cggatggccg gagtaatttc catttgtttg ctggtaatgt tcattgtcat
    19681 aaattttacc tcttgtcttc ccgtcttggt gaattcagca taccgttcct gatgtcaaaa
    19741 tgtgtgatgg aaatctcata atcttgtcac tttttgtcaa cgaatctttt ttgtgagaaa
    19801 tcgcaggaag aggtgttttt ttacttgagg aatatctcaa agcgagccat atttgatgag
    19861 atcgatagtg actaaatcgc ttcagtttca caactaacag aataaaaaaa cggcagctca
    19921 agggctgccg ttttgtgttt caggtttttg ttatgtattg ctgctgtttg cagcgatgat
    19981 tttcgctact ttttcagctt gcgcattcat ctgcatctga cggtatgcat tttccatcag
    20041 cggcagcgca tcacgcgtag cctgggtatc cgggtagtcg cgcaacatgc cttctacgcg
    20101 gttaacgacg gcaacccatg caccacgttc tgtatagtac tcggcaacgg agtattcata
    20161 tttcgccaga cgatctttca ggaataccag acgtttggtg gcatcggtgg tgtactgact
    20221 gttcggatag ccgcgcacca gtttggaaaa gtcactaaac gcagctcgtg catgttgagg
    20281 atcgcgatcg ctacggtcaa cgccaaagaa cccttgcagc gcactgtcat ccagcgccat
    20341 attggtcagg ccacgcatgt acatgacata atcgatattc ggatgggtcg ggttaaggcg
    20401 aataaaacga tcgatggcag cctgtgctaa cggcaaatcg gcgtttttat agtaggcgta
    20461 gatgagatcc agctgcacct gctgcgaata cggaccaaac ggatagcgat tatctaacgc
    20521 ttccagttgc gttattgcct gtctccagtt accgtcctgc agcttttgtt gtgcagtcgc
    20581 gtaaatttca tttggcggat tatcaggtac ttcttccttt gaccccgagc aacccgccaa
    20641 aaacaggctt agtgtggcgg ctgccaccag atatttcatg cgcgtcatga cgttttgact
    20701 ttcctcaaaa tgtaatacgg gagattctct gttcctgctc ccggttaaga ccagctacaa
    20761 tagcacacta tattaaacgg caaagccgta aaaccccaac gataaacgaa gaagcagtat
    20821 atatggcaca acgagtacag ctcactgcaa cggtgtccga aaaccaactc ggtcaacgct
    20881 tagatcaggc tttggccgaa atgttcccgg attattcacg ttcgcggata aaagaatgga
    20941 tcctcgacca gcgcgtgctg gttaacggca aagtttgtga taagccgaaa gaaaaagtat
    21001 tgggtggcga gcaggttgcc atcaacgctg agattgaaga agaagcgcgt tttgaaccgc
    21061 aggatatccc gctggatatc gtctatgaag atgacgatat catcgtcatt aataaaccgc
    21121 gcgacctggt ggtacatcct ggcgcgggta acccggatgg cacggtactg aatgcattgc
    21181 ttcattatta tccgcccatt gccgatgtac cgcgtgcagg catcgtccat cgtctggata
    21241 aagacaccac tggcctgatg gttgtggcaa aaaccgttcc ggctcagacg cgtttagtcg
    21301 aatctttgca aaggcgtgaa attactcgtg agtatgaagc ggtggcaatt ggtcatatga
    21361 ccgctggcgg tacagtagat gaaccaatca gccgccaccc gaccaaacgt acccatatgg
    21421 cggtgcatcc gatgggcaaa ccagcggtga ctcactatcg catcatggaa cacttccgtg
    21481 tgcacacgcg tctgcggttg cgtctggaaa ctggacgtac gcaccagatc cgcgtgcata
    21541 tggcccatat cactcatccg ctggtgggcg atccggttta tggtggccgt ccgcgtccgc
    21601 caaaaggtgc ttcggaagcg tttatctcca cgctgcgtaa atttgaccgc caggcgcttc
    21661 atgcaaccat gctgcgtctt tatcacccga tctccggtat cgaaatggaa tggcatgcgc
    21721 ctattccaca agatatggtg gagctgattg aggtgatgcg cgccgatttc gaagagcata
    21781 aagatgaagt ggactggtta tgagtaagct gattgtcccg cagtggccgc tgccaaaagg
    21841 tgttgcggcc tgtagctcta ctcgtatcgg cggcgtgagc ttgcccccgt atgactcgct
    21901 caacctcggt gcccattgtg gcgataaccc ggatcacgtt gaggagaatc gcaagcgact
    21961 ttttgctgcg ggcaatttgc cttctaaacc ggtctggctt gagcaggtac acggcaaaga
    22021 tgtgcttaag cttactggcg aaccttatgc ctcaaaacga gcagatgcct cttatagcaa
    22081 tacgcccggc acggtttgcg cagtgatgac tgccgactgc ctccctgtgc tgttttgcaa
    22141 tcgagcggga acggaagtcg ccgccgctca tgctggctgg cgtggactgt gcgcaggcgt
    22201 gctggaagag acagtttcct gttttgctga taatccagaa aatattctcg cctggttagg
    22261 gccggcaatt ggtccacgcg cgtttgaagt gggggcggag gttcgcgagg cgtttatggc
    22321 agtagacgcc gaagcgagta cagctttcat tcagcatggt gataagtatc tggcggacat
    22381 ttatcagctt gcccggcagc gtctggcgag cgttggtgtt gaacaaattt tcggcggcga
    22441 ccgttgtaca tatacggaaa atgagacttt cttctcttat cgtcgcgaca agaccaccgg
    22501 tcgtatggca agtttcattt ggctgatata acctaaagaa tcaagacgat ccggtacgcg
    22561 tgattttctt ttcacattaa tctggtcaat aaccttgaat aattgaggga tgacctcatt
    22621 taatctccag tagcaacttt gatccgttat gggaggagtt atgcgtctgg atcgtcttac
    22681 taataaattc cagcttgctc ttgccgatgc ccaatcactt gcactcgggc acgacaacca
    22741 attcatcgaa ccacttcatt taatgagcgc cctgctgaat caggaagggg gttcggttag
    22801 tcctttatta acatccgctg gcataaatgc tggccagttg cgcacagata tcaatcaggc
    22861 attaaatcgt ttaccgcagg ttgaaggtac tggtggtgat gtccagccat cacaggatct
    22921 ggtgcgcgtt cttaatcttt gcgacaagct ggcgcaaaaa cgtggtgata actttatctc
    22981 gtcagaactg ttcgttctgg cggcacttga gtctcgcggc acgctggccg acatcctgaa
    23041 agcagcaggg gcgaccaccg ccaacattac tcaagcgatt gaacaaatgc gtggaggtga
    23101 aagcgtgaac gatcaaggtg ctgaagacca acgtcaggct ttgaaaaaat ataccatcga
    23161 ccttaccgaa cgagccgaac agggtaaact cgatccggtg attggtcgtg atgaagaaat
    23221 tcgccgtacc attcaggtgc tgcaacgtcg tactaaaaat aacccggtac tgattggtga
    23281 accaggcgtc ggtaagactg ccatcgttga aggtctggcg cagcgtatta tcaacggcga
    23341 agtgcccgaa gggttgaaag gccgccgcgt actggcgctg gatatgggcg cgctggtggc
    23401 tggggcgaaa tatcgcggtg agtttgaaga acgtttaaaa ggcgtactta acgatcttgc
    23461 caaacaggaa ggcaacgtca tcctgtttat cgacgaatta cataccatgg tcggcgcggg
    23521 taaagccgat ggcgcaatgg acgccggaaa catgctgaaa ccggcgctgg cgcgtggtga
    23581 attgcactgc gtaggtgcca cgacgcttga cgaatatcgc cagtacatcg aaaaagatgc
    23641 tgcgctggaa cgtcgtttcc agaaagtgtt tgttgccgag ccttctgttg aagatacgat
    23701 tgcgattctg cgtggcctga aagaacgtta cgagctgcac caccatgtgc aaattactga
    23761 cccggcaatt gttgcagcgg cgacgttgtc tcatcgctac attgctgacc gtcagctgcc
    23821 agataaagcc atcgacctga tcgatgaggc agcatccagc attcgtatgc agattgactc
    23881 aaaaccagaa gaactcgacc gactcgatcg ccgtatcatc cagctcaaac tggaacaaca
    23941 ggcgttaatg aaagagtctg atgaagccag taaaaaacgt ctggatatgc tcaatgaaga
    24001 actgagcgac aaagaacgtc agtactccga gttagaagaa gagtggaaag cagagaaagc
    24061 atcgctttct ggtacgcaga ccattaaagc ggaactggaa caggcgaaaa tcgctattga
    24121 acaggctcgc cgtgtgggtg acctggcgcg gatgtctgaa ctgcaatacg gcaaaatccc
    24181 ggaactggaa aagcaactgg aagccgcaac gcagctcgaa ggcaaaacta tgcgtctgtt
    24241 gcgtaataaa gtgaccgatg ccgaaattgc tgaagtgctg gcgcgttgga cggggattcc
    24301 ggtttctcgc atgatggaaa gcgagcgcga aaaactgctg cgtatggagc aagaactgca
    24361 ccatcgcgtt attggtcaga acgaagcggt tgatgcggta tctaacgcta ttcgtcgtag
    24421 ccgtgcgggg ctggcggatc caaatcgccc gattggttca ttcctgttcc tcggcccaac
    24481 gggtgtgggg aaaactgagc tttgtaaggc gctggcgaac tttatgtttg atagcgacga
    24541 ggcgatggtc cgtatcgata tgtccgagtt tatggagaaa cactcggtgt ctcgtctggt
    24601 tggtgcgcct ccgggatatg tcggttatga agaaggtggc tacctgaccg aagcggtgcg
    24661 tcgtcgtccg tattccgtca tcctgctgga tgaagtggaa aaagcgcatc cggatgtctt
    24721 caacattctg ttgcaggtac tggatgatgg gcgtctgact gacgggcagg ggagaacggt
    24781 cgacttccgt aatacggttg tcattatgac ctctaacctc ggttccgatt tgattcagga
    24841 acgcttcggt gaactggatt atgcgcacat gaaagagctg gtgctcggtg tggtaagcca
    24901 taacttccgt ccggaattca ttaaccgtat cgatgaagtg gtggtcttcc atccgctggg
    24961 tgaacagcac attgcgtcga ttgcgcagat tcagttgaaa cgtctgtata aacgtctgga
    25021 agaacgtggt tatgaaatcc acatttctga cgaggcgctg aaactgctga gcgagaacgg
    25081 ttacgatccg gtctatggtg cacgtcctct gaaacgtgca attcagcagc agatcgaaaa
    25141 cccgctggca cagcaaatac tgtctggtga attggttccg ggtaaagtga ttcgcctgga
    25201 agttaatgaa gaccggattg tcgccgtcca gtaaatgata aaacgagccc ttcggggctc
    25261 gtttttgtct ataagttaga cgggaaagac tatatttaag atgttttgcc tgaaaaatga
    25321 gcgaacgata aagtttttat attttcctct tgtcaggccg gaataactcc ctataatgcg
    25381 ccaccactga cacagaacaa cggcaaacac gccgccgggt cagcggggtt ctcctgagaa
    25441 tctcaacaga gaaagcaaaa a
//
LOCUS       DAMZFT010000070        25389 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.070, whole genome shotgun sequence.
ACCESSION   DAMZFT010000070 DAMZFT010000000
VERSION     DAMZFT010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 25389)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25389)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25389
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.070"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..1420
                     /gene="proS"
                     /locus_tag="PN977_002911"
     CDS             <1..1420
                     /gene="proS"
                     /locus_tag="PN977_002911"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HDI6042928.1"
                     /translation="DRPFVLGPTHEEVITDLIRNELSSYKQLPLNFYQIQTKFRDEVR
                     PRFGVMRSREFLMKDAYSFHTSQESLQETYDAMYAAYSKIFSRMGLDFRAVQADTGSI
                     GGSASHEFQVLAQSGEDDVVFSDTSDYAANIELAEAIAPKEPRAAATQEMTLVDTPNA
                     KTIAELVEQFNLPIEKTVKTLLVKAVEGSSFPLVALLVRGDHELNEVKAEKLPQVASP
                     LTFATEEEIRAVVKAGPGSLGPVNMPIPVVIDRTVAAMSDFAAGANIDGKHYFGINWD
                     RDVATPEIADIRNVVAGDPSPDGQGTLLIKRGIEVGHIFQLGTKYSEALKASVQGEDG
                     RNQILTMGCYGIGVTRVVAAAIEQNYDERGIVWPDAIAPFQVAILPMNMHKSFRVQEL
                     AEKLYSELRAQGIEVLLDDRKERPGVMFADMELIGIPHTIVLGDRNLDNDDIEYKYRR
                     NGEKQLIKTGDIVDYLVKQIKG"
     gene            1473..2297
                     /locus_tag="PN977_002912"
     CDS             1473..2297
                     /locus_tag="PN977_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeF family permuted papain-like enzyme"
                     /protein_id="HDI6042929.1"
                     /translation="MDKPKAYCRLLLPCFLLLSACTVDISQPDSSATAVNAEAKTWAV
                     KFQHQSSFTEQSIKEITEPDLKPGDLLFSSSLGVTSFGIRVFSTSSVSHVAIYLGDNN
                     VAEATGAGVQIVSLKKAMKHSDKLFVLRVPDLTPQQATEITAFANKIKDSGYNYRGIV
                     EFIPFMVTRQMCSLNPFSEDFRQQCVSSLAKAQLSNVGEGDKKSWFCSEFVTDAFAKS
                     GHPLTLAQSGWISPADLMHMRSGDVSAFKPETQLQYIGHLKPGIYIKASRFVGLTQ"
     gene            complement(2453..3163)
                     /gene="nlpE"
                     /locus_tag="PN977_002913"
                     /gene_synonym="cutF"
     CDS             complement(2453..3163)
                     /gene="nlpE"
                     /locus_tag="PN977_002913"
                     /gene_synonym="cutF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001591565.1"
                     /note="NlpE, an outer membrane lipoprotein, interacts
                     directly with CpxA, the sensor histidine kinase of the Cpx
                     system for response to envelope stress; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response activation lipoprotein
                     NlpE"
                     /protein_id="HDI6042930.1"
                     /translation="MVRKAIVTAMAVISLFTLMGCNNRAEVDTLSPAQAAELKPMPQS
                     WRGVLPCADCEGIETSLFLEKDGTWVMSERYLGAREEPSSFASYGTWARTADKLVLTD
                     SKGEKSYYRAKGDALEMLDREGNPIESQFNYTLEPAKSSMPMTPMTLRGMYFYMADAA
                     TFTDCATGKRFIVANNAELERGYLAARGNSEKPVLLSVEGHFTLEANPDTGAPTKVLA
                     PDTAGKFYPNHDCSSLGL"
     gene            complement(3177..3599)
                     /gene="arfB"
                     /locus_tag="PN977_002914"
     CDS             complement(3177..3599)
                     /gene="arfB"
                     /locus_tag="PN977_002914"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HDI6042931.1"
                     /translation="MIVISRHVAIPDGELEITAIRAQGAGGQHVNKTSTAIHLRFDIR
                     ASSLPEYYKERLLAASHHLISSDGVIVIKAQEYRSQELNREAALARLVAVIKELTTEQ
                     KARRPTRPTRASKERRLASKAQKSSVKAMRGKVRSGRE"
     gene            complement(3596..4141)
                     /locus_tag="PN977_002915"
     CDS             complement(3596..4141)
                     /locus_tag="PN977_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeQ family protein"
                     /protein_id="HDI6042932.1"
                     /translation="MALKATIYKATVNVADLDRNQFLDASLTLARHPSETQERMMLRL
                     LAWLKYADERLQFTRGLCADDEPEAWLRNDHLGIDLWIELGLPDERRIKKACTQAAEV
                     ALFAYNSRAAQIWWQQNQSKCAQFANLSVWYLDDEQLAKVSAFADRTMTLQATIQDGV
                     IWLSDDKNNLEVNLTAWQQPS"
     gene            4307..4507
                     /locus_tag="PN977_002916"
     CDS             4307..4507
                     /locus_tag="PN977_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeP family protein"
                     /protein_id="HDI6042933.1"
                     /translation="MEKYCELIRKRYAEIASGDLGYVPDALGCVLKVLNEMAADDALS
                     EAVREKAAYAAANLLVSDYVNE"
     gene            4494..4754
                     /gene="rof"
                     /locus_tag="PN977_002917"
     CDS             4494..4754
                     /gene="rof"
                     /locus_tag="PN977_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rho-binding antiterminator"
                     /protein_id="HDI6042934.1"
                     /translation="MSMNDTYQPINCDDYDNLELACQHHLMLTLELKDGEKLQAKASD
                     LVSRKNVEYLVVEAAGTTRELRLDKITSFSHPEIGTVVVSES"
     gene            complement(4803..6101)
                     /gene="tilS"
                     /locus_tag="PN977_002918"
     CDS             complement(4803..6101)
                     /gene="tilS"
                     /locus_tag="PN977_002918"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HDI6042935.1"
                     /translation="MTLTLNRQLLSSRQILVAFSGGLDSTVLLHQLVQWRTENPGVTL
                     RAIHVHHGLSANADAWVRHCENICQQWQVPLVVERVQLAQEGLGIEAQARQVRYQAFA
                     RTLLPGEVLVTAQHLDDQCETFLLALKRGSGPAGLSAMGEVSEFAGTQLIRPLLARTR
                     GELEQWALAHGLRWIEDESNQDDSYDRNFLRLRVVPLLQQRWPHFAEATARSAALCAE
                     QESLLDELLADDLAHCQTPQGTLQIAPMLAMSDARRAAIIRRWLAGQNAPMPSRDALV
                     RIWQEVALAREDASPCLRLGAFEIRRYQSQLWWIKSVAGQSETIVPWQTWLQPLELPA
                     GLGSVQLTAGGDIRPPRADEAVSVRFKAPGLLHIVGRNGGRKLKKIWQELGVPPWLRD
                     TTPLLFYGETLIAAAGVFVTQEGVAEGENGVSFVWQKTLS"
     gene            complement(6166..6555)
                     /locus_tag="PN977_002919"
     CDS             complement(6166..6555)
                     /locus_tag="PN977_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDI6042936.1"
                     /translation="MLGLKQVHHIAIIATDYAVSKAFYCDILGFTLQSEVYREARDSW
                     KGDLALNGQYVIELFSFPFPPERPSRPEACGLRHLAFSVDDIDAAVAYLESHNVKCEA
                     IRVDPYTQKRFTFFNDPDGLPLELYEQ"
     gene            complement(6612..8753)
                     /gene="ldcC"
                     /locus_tag="PN977_002920"
     CDS             complement(6612..8753)
                     /gene="ldcC"
                     /locus_tag="PN977_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylase LdcC"
                     /protein_id="HDI6042937.1"
                     /translation="MNIIAIMGPHGVFYKDEPIKELESALVAQGFQIIWPQNSVDLLK
                     FIEHNPRICGVIFDWDEYSLDLCSDINQLNEYLPLYAFINTHSTMDVSVQDMRMALWF
                     FEYALGQAEDIAIRMRQYTNEYLDNITPPFTKALFTYVKERKYTFCTPGHMGGTAYQK
                     SPVGCLFYDFFGGNTLKADVSISVTELGSLLDHTGPHLEAEEYIARTFGAEQSYIVTN
                     GTSTSNKIVGMYAAPSGSTLLIDRNCHKSLAHLLMMNDVVPVWLKPTRNALGILGGIP
                     RREFSRDSIEEKVAATTQAQWPVHAVITNSTYDGLLYNTDWIKQTLDVPSIHFDSAWV
                     PYTHFHPIYQGKSGMSGERVAGKVIFETQSTHKMLAALSQASLIHIKGEYDEEAFNEA
                     FMMHTTTSPSYPIVASVETAAAMLRGNPGKRLINRSVERALHFRKEVQRLREESDGWF
                     FDIWQPPQVDEAECWPVAPGEQWHGFNDADADHMFLDPVKVTILTPGMDEQGNMSEEG
                     IPAALVAKFLDERGIVVEKTGPYNLLFLFSIGIDKTKAMGLLRGLTEFKRSYDLNLRI
                     KNMLPDLYAEDPDFYRSMRIQDLAQGIHKLIRKHDLPGLMLRAFDTLPEMIMTPHQAW
                     QRQIKGEVETIALEQLVGRVSANMILPYPPGVPLLMPGEMLTEESRTVLDFLLMLCSV
                     GQHYPGFETDIHGAKQDEDGIYRVRVLKMAG"
     gene            complement(8852..9811)
                     /gene="accA"
                     /locus_tag="PN977_002921"
     CDS             complement(8852..9811)
                     /gene="accA"
                     /locus_tag="PN977_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HDI6042938.1"
                     /translation="MSLNFLDFEQPIAELEAKIDSLTAVSRQDEKLDINIDEEVHRLR
                     EKSVELTRKIFADLGAWQIAQLARHPQRPYTLDYVRLAFDEFDELAGDRAYADDKAIV
                     GGIARLDGRPVMIIGHQKGRETKEKIRRNFGMPAPEGYRKALRLMQMAERFKMPIITF
                     IDTPGAYPGVGAEERGQSEAIARNLREMSRLSVPTICTVIGEGGSGGALAIGVGDKVN
                     MLQYSTYSVISPEGCASILWKSADKAPLAAEAMGIIAPRLKELKLIDSIIPEPLGGAH
                     RNPEAMAASLKAQLLADLADLDVLSTEDLKNRRYQRLMSYGYA"
     gene            complement(9824..13306)
                     /gene="dnaE"
                     /locus_tag="PN977_002922"
     CDS             complement(9824..13306)
                     /gene="dnaE"
                     /locus_tag="PN977_002922"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HDI6042939.1"
                     /translation="MSEPRFVHLRVHSDYSMIDGLAKTAPLVKKAAALGMPALAITDF
                     TNLCGLVKFYGAGHGAGIKPIVGADFNVQCDLLGDELTHLTVLAANNTGYQNLTLLIS
                     KAYQRGYGAAGPIIDRDWLIELNEGLILLSGGRMGDVGRSLLRGNSALVDECVAFYEE
                     HFPDRYFLELIRTGRPDEESYLHAAVELAESRGLPVVATNDVRFIDSSDFDAHEIRVA
                     IHDGFTLDDPKRPRNYSPQQYMRSEEEMCELFADIPEALANTVEIAKRCNVTVRLGEY
                     FLPQFPTGDMSTEDYLVKRAKEGLEERLAFLFPDEEERLKRRPEYDERLETELQVINQ
                     MGFPGYFLIVMEFIQWSKDNGVPVGPGRGSGAGSLVAYALKITDLDPLEFDLLFERFL
                     NPERVSMPDFDVDFCMEKRDQVIEHVADMYGRDAVSQIITFGTMAAKAVIRDVGRVLG
                     HPYGFVDRISKLIPPDPGMTLAKAFEAEPQLPEIYEADEEVKALIDMARKLEGVTRNA
                     GKHAGGVVIAPTKITDFAPLYCDEEGKHPVTQFDKSDVEYAGLVKFDFLGLRTLTIIN
                     WALEMINKRRAKNGEPPLDIAAIPLDDKKSFDMLQRSETTAVFQLESRGMKDLIKRLQ
                     PDCFEDMIALVALFRPGPLQSGMVDNFIDRKHGREEISYPDVQWQHESLKPVLEPTYG
                     IILYQEQVMQIAQVLSGYTLGGADMLRRAMGKKKPEEMAKQRSVFAEGAEKNGINAEL
                     AMKIFDLVEKFAGYGFNKSHSAAYALVSYQTLWLKAHYPAEFMAAVMTADMDNTEKVV
                     GLVDECWRMGLKILPPDINSGLYHFHVNDDGEIVYGIGAIKGVGEGPIEAIIEARNKG
                     GYFRELFDLCARTDTKKLNRRVLEKLIMSGAFDRLGPHRAALMNSLGDALKAADQHAK
                     AEAIGQADMFGVLAEEPEQIEQSYASCQPWPEQVVLDGERETLGLYLTGHPINQYLKE
                     IERYVGGIRLKDMHPTERGKVITAAGLVVAARVMVTKRGNRIGICTLDDRSGRLEVML
                     FTDALDKYQQLLEKDRILIVSGQVSFDDFSGGLKMTAREVMDIDEAREKYARGLAISL
                     TDRQIDDQLLNRLRQSLEPHRSGTIPVHLYYQRADARARLRFGATWRVSPSDRLLNDL
                     RGLIGSEQVELEFD"
     gene            complement(13343..13939)
                     /gene="rnhB"
                     /locus_tag="PN977_002923"
     CDS             complement(13343..13939)
                     /gene="rnhB"
                     /locus_tag="PN977_002923"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HDI6042940.1"
                     /translation="MIEFVYPHTQLVAGVDEVGRGPLVGAVVTAAVILDPARPIAGLN
                     DSKKLSEKRRLALCEEIKEKALSWSLGRAEPHEIDELNILHATMLAMQRAVAGLHIAP
                     EYVLIDGNRCPKLPMPSMAVVKGDSRVPEISAASILAKVTRDAEMAALDIVFPQYGFA
                     QHKGYPTAFHLEKLAEHGATEHHRRSFGPVKRALGLAS"
     gene            complement(13936..15084)
                     /gene="lpxB"
                     /locus_tag="PN977_002924"
     CDS             complement(13936..15084)
                     /gene="lpxB"
                     /locus_tag="PN977_002924"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706127.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HDI6042941.1"
                     /translation="MTEQRPLTIALVAGETSGDILGAGLIRALKERVPNARFVGVAGP
                     RMQAEGCEAWYEMEELAVMGIVEVLGRLRRLLHIRADLTKRFGELKPDVFVGIDAPDF
                     NITLEGNLKKQGVKTIHYVSPSVWAWRQKRVFKIGRATDLVLAFLPFEKAFYDKYNVP
                     CRFIGHTMADAMPLDPDKNGARDVLGIPYDAHCLALLPGSRGAEVEMLSADFLKTAQL
                     LRQTYPDLEIVVPLVNAKRREQFERIKAEVAPDLAVHLLDGMGREAMVASDAALLASG
                     TAALECMLAKCPMVVGYRMKPFTFWLAKRLVKTEYVSLPNLLAGRELVKELLQEECEP
                     QKLAAALLPLLANGKTSHAMHDTFRELHQQIRCNADEQAAQAVLELAQ"
     gene            complement(15084..15872)
                     /gene="lpxA"
                     /locus_tag="PN977_002925"
     CDS             complement(15084..15872)
                     /gene="lpxA"
                     /locus_tag="PN977_002925"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HDI6042942.1"
                     /translation="MIDKSAFVHPTAIVEEGASIGANAHIGPFCIVGPHVEIGEGTVL
                     KSHVVVNGHTKIGRDNEIYQFASIGEVNQDLKYAGEPTRVEIGDRNRIRESVTIHRGT
                     VQGGGLTKVGSDNLLMINAHIAHDCTVGNRCILANNATLAGHVSVDDFAIIGGMTAVH
                     QFCIIGAHVMVGGCSGVAQDVPPYVIAQGNHATPFGVNIEGLKRRGFSREAITAIRNA
                     YKLIYRSGKTLDEVKPEIAELAETYPEVKAFTDFFARSTRGLIR"
     gene            complement(15876..16331)
                     /gene="fabZ"
                     /locus_tag="PN977_002926"
     CDS             complement(15876..16331)
                     /gene="fabZ"
                     /locus_tag="PN977_002926"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000210741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HDI6042943.1"
                     /translation="MTTNTHTLQIEEILELLPHRFPFLLVDRVLDFEEGRFLRAVKNV
                     SVNEPFFQGHFPGKPIFPGVLILEAMAQATGILAFKSVGKLEPGELYYFAGIDEARFK
                     RPVVPGDQMIMEVTFEKTRRGLTRFKGVALVDGKVVCEATMMCARSREA"
     gene            complement(16436..17461)
                     /gene="lpxD"
                     /locus_tag="PN977_002927"
     CDS             complement(16436..17461)
                     /gene="lpxD"
                     /locus_tag="PN977_002927"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HDI6042944.1"
                     /translation="MPSIRLADLAQQLDAELHGDGDIVITGVASMQSAQTGHITFMVN
                     PKYREHLGLCQASAVVMTQDDLPFAKSAALVVKNPYLTYARMAQILDTTPQPAQNIAP
                     SAVIDATAKLGNNVSIGANAVIESGVELGDNVIIGAGCFVGKNSKIGAGSRLWANVTI
                     YHEIQIGQNCLIQSGTVVGADGFGYANDRGNWVKIPQIGRVIIGDRVEIGACTTIDRG
                     ALDDTVIGNGVIIDNQCQIAHNVVIGDNTAVAGGVIMAGSLKIGRYCMIGGASVINGH
                     MEICDKVTVTGMGMVMRPITEPGVYSSGIPLQPNKVWRKTAALVMNIDDMSKRLKSLE
                     RKVNQQD"
     gene            complement(17465..17950)
                     /gene="skp"
                     /locus_tag="PN977_002928"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
     CDS             complement(17465..17950)
                     /gene="skp"
                     /locus_tag="PN977_002928"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone Skp"
                     /protein_id="HDI6042945.1"
                     /translation="MKKWLLAAGLGLALATSAQAADKIAIVNMGSLFQQVAQKTGVSN
                     TLENEFKGRASELQRMETDLQAKMKKLQSMKAGSDRTKLEKDVMAQRQTFAQKAQAFE
                     QDRARRSNEERGKLVTRIQTAVKSVANSQDIDLVVDANAVAYNSSDVKDITADVLKQV
                     K"
     gene            complement(18072..20504)
                     /gene="bamA"
                     /locus_tag="PN977_002929"
     CDS             complement(18072..20504)
                     /gene="bamA"
                     /locus_tag="PN977_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HDI6042946.1"
                     /translation="MAMKKLLIASLLFSSATVYGAEGFVVKDIHFEGLQRVAVGAALL
                     SMPVRTGDTVNDEDISNTIRALFATGNFEDVRVLRDGDTLLVQVKERPTIASITFSGN
                     KSVKDDMLKQNLEASGVRVGESLDRTTIADIEKGLEDFYYSVGKYSASVKAVVTPLPR
                     NRVDLKLVFQEGVSAEIQQINIVGNHAFTTDELISHFQLRDEVPWWNVVGDRKYQKQK
                     LAGDLETLRSYYLDRGYARFNIDSTQVSLTPDKKGIYVTVNITEGDQYKLSGVEVSGN
                     LAGHSAEIEQLTKIEPGELYNGTKVTKMEDDIKKLLGRYGYAYPRVQSMPEINDADKT
                     VKLRVNVDAGNRFYVRKIRFEGNDTSKDAVLRREMRQMEGAWLGSDLVDQGKERLNRL
                     GFFETVDTDTQRVPGSPDQVDVVYKVKERNTGSFNFGIGYGTESGVSFQAGVQQDNWL
                     GTGYAVGINGTKNDYQTYAELSVTNPYFTVDGVSLGGRLFYNDFQADDADLSDYTNKS
                     YGTDVTLGFPINEYNSLRAGLGYVHNSLSNMQPQVAMWRYLYSMGEHPSTSDQDNSFK
                     TDDFTFNYGWTFNKLDRGYFPTDGSRVNLTGKVTIPGSDNEYYKVTLDTATYVPIDDD
                     HKWVVLGRTRWGYGDGLGGKEMPFYENFYAGGSSTVRGFQSNTIGPKAVYFPYSPNGY
                     DPSNDYACATQDGAKDLCKSDDAVGGNAMAVASLEFITPTPFISDKYANSVRISFFWD
                     MGTVWDTNWDSSQYSGYPDYSDPSNIRMSAGIALQWMSPLGPLVFSYAQPFKKYDGDK
                     AEQFQFNIGKTW"
     gene            complement(20534..21886)
                     /gene="rseP"
                     /locus_tag="PN977_002930"
     CDS             complement(20534..21886)
                     /gene="rseP"
                     /locus_tag="PN977_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma E protease regulator RseP"
                     /protein_id="HDI6042947.1"
                     /translation="MLSFLWDLASFIVALGVLITVHEFGHFWVARRCGVRVERFSIGF
                     GKALWRRTDKLGTEYVIALIPLGGYVKMLDERAEPVVPELRHHAFNNKSVGQRAAIIA
                     AGPVANFIFAIFAYWLVFIIGVPGVRPVVGEIAANSIAAEAQIAPGTELKAVDGIETP
                     DWDAVRLQLVDKIGDESTTITVAPFGSDQRRDVKLDLRHWAFEPDKEDPVTSLGIRPR
                     GPQIEPVLENVQPNSAASKAGLQAGDRIVKVDGQPLTQWVTFVMLVRDNPGKSLALEI
                     ERQGSPLSLTLIPESKPGNGKAIGFVGIEPKVIPLPDEYKVVRQYGPFNAIVEATDKT
                     WQLMKLTVSMLGKLITGDVKLNNLSGPISIAKGAGMTAELGVVYYLPFLALISVNLGI
                     INLFPLPVLDGGHLLFLAIEKIKGGPVSERVQDFCYRIGSILLVLLMGLALFNDFSRL
                     "
     gene            complement(21898..22755)
                     /gene="cdsA"
                     /locus_tag="PN977_002931"
     CDS             complement(21898..22755)
                     /gene="cdsA"
                     /locus_tag="PN977_002931"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HDI6042948.1"
                     /translation="MLKYRLISAFVLIPVVIAALFLLPPVGFAIVTLVVCMLAAWEWG
                     QLSGFTTRSQRVWLAVLCGLLLALMLFLLPEYHRNIHQPLVEISLWASLGWWIVALLL
                     VLFYPGSAAIWRNSKTLRIIFGVLTIVPFFWGMLALRAWHYDENHYSGAIWLLYVMIL
                     VWGADSGAYMFGKLFGKHKLAPKVSPGKTWQGFIGGLATAAVISWGYGMWVNLDVAPV
                     TLLICSIVAALASVLGDLTESMFKREAGIKDSGHLIPGHGGILDRIDSLTAAVPVFAC
                     LLLLVFRTL"
     gene            complement(22768..23526)
                     /gene="ispU"
                     /locus_tag="PN977_002932"
                     /gene_synonym="uppS"
     CDS             complement(22768..23526)
                     /gene="ispU"
                     /locus_tag="PN977_002932"
                     /gene_synonym="uppS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308203.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl-diphosphate-specific
                     ditrans,polycis-undecaprenyl-diphosphate synthase"
                     /protein_id="HDI6042949.1"
                     /translation="MLSATQPLSEKLPAHGCRHVAIIMDGNGRWAKKQGKIRAFGHKA
                     GAKSVRRAVSFAANNGIEALTLYAFSSENWNRPAQEVSALMELFVWALDSEVKSLHRH
                     NVRLRIIGDTSRFNSRLQERIRKSEALTAGNTGLTLNIAANYGGRWDIVQGVRQLAEK
                     VQQGNLQPDQIDEEMLNQHVCMHELAPVDLVIRTGGEHRISNFLLWQIAYAELYFTDV
                     LWPDFDEQDFEGALNAFANRERRFGGTEPGDETA"
     gene            complement(23715..24911)
                     /gene="ispC"
                     /locus_tag="PN977_002933"
     CDS             complement(23715..24911)
                     /gene="ispC"
                     /locus_tag="PN977_002933"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HDI6042950.1"
                     /translation="MKQLTILGSTGSIGCSTLDVVRHNPEHFRVVALVAGKNVTRMVE
                     QCLEFSPRYAVMDDEASAKLLKTMLQQQGSRTEVLSGQQAACDMAALEDVDQVMAAIV
                     GAAGLLPTLAAIRAGKTILLANKESLVTCGRLFMDAVKQSKAQLLPVDSEHNAIFQSL
                     PQPIQHNLGYADLEQNGVVSILLTGSGGPFRETPLRDLATMTPDQACRHPNWSMGRKI
                     SVDSATMMNKGLEYIEARWLFNASASQMEVLIHPQSVIHSMVRYQDGSVLAQLGEPDM
                     RTPIAHTMAWPNRVNSGVKPLDFCKLSALTFAAPDYDRYPCLKLAMEAFEQGQAATTA
                     LNAANEITVAAFLAQQIRFTDIAALNLSVLEKMDMREPQCVDDVLSVDANAREVARKE
                     VMRLAS"
     gene            complement(25003..>25389)
                     /gene="frr"
                     /locus_tag="PN977_002934"
     CDS             complement(25003..>25389)
                     /gene="frr"
                     /locus_tag="PN977_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HDI6042951.1"
                     /translation="TVEDSRTLKINVFDRSMSPAVEKAIMASDLGLNPNSAGSDIRVP
                     LPPLTEERRKDLTKIVRGEAEQARVAVRNVRRDANDKVKALLKDKEISEDDDRRSQDD
                     VQKLTDAAIKKIEAALADKEAELMQF"
BASE COUNT         6106 a         7103 c         6157 g         6023 t
ORIGIN      
        1 tgaccgtccg ttcgtacttg gcccaactca tgaagaagtg attaccgacc tgattcgtaa
       61 cgagctgagc tcttacaaac agctgccgct gaacttctat cagatccaga ccaagttccg
      121 cgacgaagtg cgtccgcgtt tcggcgtcat gcgttcccgc gaattcctga tgaaagatgc
      181 ttactctttc catacttctc aggaatcctt acaggaaacc tacgatgcaa tgtatgcggc
      241 ctacagcaaa atcttcagcc gcatggggct ggatttccgc gccgtacagg ccgacaccgg
      301 ttctatcggc ggtagcgcct ctcacgaatt ccaggtgctg gcgcagagcg gtgaagacga
      361 tgtggtcttc tccgacacct ctgactatgc agcgaacatt gagctggcag aagctatcgc
      421 gccgaaagaa ccgcgcgctg ctgctaccca ggaaatgacg ctggttgata cgccgaacgc
      481 gaaaaccatc gcggaactgg ttgaacagtt caatctgccg attgagaaaa cggttaagac
      541 tctgctggtt aaagccgttg aaggcagtag cttcccgctg gttgcgctgc tggtgcgcgg
      601 tgaccacgag ctgaacgaag ttaaagcaga aaaactgccg caggttgcca gcccgctgac
      661 ttttgcgacc gaagaagaaa ttcgtgccgt ggttaaagcc ggtccgggtt cactgggtcc
      721 ggtaaacatg ccgattccgg tggtgattga ccgtaccgtt gcggcgatga gtgatttcgc
      781 tgctggtgct aacatcgatg gtaaacacta cttcggtatc aactgggatc gcgatgtcgc
      841 taccccggaa attgctgata tccgtaacgt ggtggctggc gatccaagcc cggatggtca
      901 gggtacgctg ctgatcaaac gtggtatcga agtcggtcac atcttccagc tgggtaccaa
      961 gtactccgaa gcactgaaag cctccgtaca gggtgaagat ggccgtaacc aaatcctgac
     1021 gatgggttgc tacggtatcg gtgtaacgcg tgtggtagca gcggcgattg agcagaacta
     1081 cgacgaacgc ggcatcgtat ggcctgacgc tatcgcgccg ttccaggtgg cgattctgcc
     1141 aatgaacatg cacaaatcct tccgcgtaca ggaacttgct gagaaactgt acagcgaact
     1201 gcgcgcacaa ggtatcgaag tgctgctgga tgaccgcaaa gagcgtccgg gcgtgatgtt
     1261 tgctgatatg gaactgatcg gtattccgca cactatcgtg ctgggcgacc gtaacctcga
     1321 caacgacgat atcgaatata aatatcgtcg taacggcgag aaacagttaa ttaagactgg
     1381 tgacatcgtc gattatctgg tgaaacagat taaaggctga tgccagaaag ggtcctgaat
     1441 ttcggggccc ttttttacat ggattgtata aaatggataa accaaaggcg tactgccgcc
     1501 tgcttctccc ctgctttctt ttactttcgg cctgtaccgt tgatatcagc cagccagact
     1561 catcagccac agcggtaaat gccgaggcga aaacatgggc tgttaaattc cagcatcaaa
     1621 gctctttcac cgaacaatca ataaaagaaa taacagaacc agatctcaaa cccggcgatt
     1681 tgcttttctc ctcaagcctt ggggtaacct catttggtat ccgcgtattc agcacttcct
     1741 ctgtgagtca cgttgcaatc tatttgggag ataataacgt tgccgaagcg acaggcgctg
     1801 gcgtccagat tgtttcactt aaaaaagcca tgaagcatag tgataagctt ttcgtcttac
     1861 gagtcccgga ccttaccccg caacaagcca cagaaatcac cgcgttcgcc aataaaatca
     1921 aagatagcgg ttataactat cgcggcattg tcgaatttat tccatttatg gtgactcgcc
     1981 agatgtgctc actgaatccg ttctccgagg attttcgcca acagtgcgtc agcagcctgg
     2041 cgaaagcgca gttaagtaac gttggcgaag gagacaaaaa gtcgtggttt tgttcggaat
     2101 ttgtcactga tgcatttgcc aaatccgggc atccgcttac gctggcgcaa tcgggctgga
     2161 ttagccctgc cgatctgatg catatgcgta gtggtgatgt ctctgcattt aaaccagaaa
     2221 cgcagttaca gtatattggg cacctgaagc cagggattta tattaaagca agtcgctttg
     2281 ttggactgac tcagtgataa aagccagcct cgtgcgcaaa atgccggatg cggcgtgaac
     2341 gccttattcg gcctacaaaa tcgtgctaat tcaaaatatt gcattgtatg ctttaggcct
     2401 gataagcgca gcgcatgagg caatatttca tttgtttctg tctcaagacg ggttacagcc
     2461 ccaaactact gcaatcatgg ttcgggtaaa atttacccgc cgtatcgggc gctaatactt
     2521 tagtcggcgc accggtatcg ggattagcct ccagcgtaaa gtgaccttca actgacagta
     2581 acactggttt ttcactgtta ccccgcgcag ccaggtagcc acgctccagc tctgcgttat
     2641 tcgccacaat aaaacgtttt ccggtcgcgc aatcagtaaa ggtcgccgca tcagccatat
     2701 agaaatacat gccacgcagg gtcatcggcg tcattggcat gctcgatttt gccggttcca
     2761 gcgtgtagtt gaactgcgat tcaatgggat taccttcacg atcgagcatc tccagtgcat
     2821 cccctttcgc tcgataatat gacttttcac ctttgctgtc ggttaatacc agcttgtcgg
     2881 cagttcgcgc ccatgtaccg taggaggcaa aggatgaagg ctcttcacga gcgccaagat
     2941 agcgctcgct catcacccag gttccatctt tttcgaggaa cagagaggtt tcgatccctt
     3001 cgcaatcggc acacggcagc acgccgcgcc aactttgcgg catcggtttc agttcggcag
     3061 cctgcgccgg agaaagcgtg tcgacttcgg cccgattgtt acatcccatc agagtaaaga
     3121 ggctgattac agccatcgct gtcactatcg cttttctcac catccattcc ttctttttac
     3181 tcccgaccgc tgcgcacttt gccgcgcatc gccttcacgc ttgatttttg tgccttcgat
     3241 gccagcctgc gctcttttga tgcgcgggtg ggccgcgtgg gtcgtcgggc tttttgttct
     3301 gttgttaatt ctttaatcac tgccaccaac cgggccagcg ccgcttcgcg gttgagctcc
     3361 tgactacggt attcctgcgc cttaatgaca atcacgccat cgctgctgat taaatgatgg
     3421 ctggcggcga gcaggcgctc tttgtaatac tctggcaggc tggacgcccg aatgtcaaaa
     3481 cgcagatgaa tggccgttga ggtcttatta acatgctgcc cgcccgcgcc ctgcgcacga
     3541 atggcggtga tctcaagctc accatcgggg atagcaacat gtcgggaaat cacaatcatg
     3601 aaggttgttg ccaggcggtt aagttcactt ccagattatt cttatcatcc gataaccaga
     3661 tcacgccatc ctgaatcgtt gcctgcagcg tcatggtacg atcggcaaag gcgctcactt
     3721 tcgccagctg ctcgtcgtcc agataccaga cggaaagatt ggcaaactgc gcacatttgc
     3781 tctgattttg ctgccaccag atttgcgccg cccgactatt ataggcaaac agcgccactt
     3841 ctgcggcctg agtgcaggct ttcttaatcc gtcgctcatc cggcagcccc agttcaatcc
     3901 ataaatcaat gcccaggtga tcgttacgca gccacgcttc cggctcatca tcggcacaca
     3961 aacctcgggt aaattgcaga cgttcatcag catatttcag ccacgccagc aggcgcagca
     4021 tcatgcgctc ctgggtttct gaaggatggc gcgccagcgt cagagaggca tcgagaaact
     4081 ggttgcggtc gagatcggcc acattaaccg tcgctttata aattgtcgct ttaagcgcca
     4141 taagaaaact cctttcgaat caggaggcca ttgtaacgga atacgtgcag ttgcgacgct
     4201 gatattgcag aaatgagtgt ggtatagtca ccttgcgaaa cagagtttat cttcgtaggc
     4261 ttagacttgc atccacggtt aagtcagagt gctgacagga gggcatgtgg aaaaatattg
     4321 tgagttaata cgcaagcggt acgcggaaat cgccagcgga gacttaggat acgttccgga
     4381 cgcgctgggc tgcgttttga aagtgctgaa tgaaatggct gccgacgacg ccctttctga
     4441 ggctgtcagg gaaaaagcag catacgctgc ggcaaactta ctggtgagcg attatgtcaa
     4501 tgaatgatac gtatcaacca atcaattgtg atgattacga taatcttgag ctcgcctgcc
     4561 agcatcattt aatgctgaca cttgagctga aagatggcga aaaattgcag gcgaaggcca
     4621 gcgatttagt ctcccgcaaa aatgtggagt atctggtcgt cgaggccgct ggtacaaccc
     4681 gcgagctgcg tctggataaa attaccagct ttagccaccc ggaaatcggt acggtggtgg
     4741 tgagcgagtc ctgattgact ttcgccggat gcgacgtttg atgcgtctta tccggctttc
     4801 acttaactaa gcgttttctg ccagacaaaa ctgacgccat tctcaccttc agccacacct
     4861 tcttgcgtca caaatacccc tgccgccgca atcaacgttt cgccataaaa cagcagtggc
     4921 gtggtgtcac gtagccacgg cggcacaccc agctcttgcc agattttctt cagcttacgt
     4981 ccgccattgc gcccgacaat atgcagcaat ccaggcgctt tgaaacgcac gctgaccgct
     5041 tcgtctgcac gcggagggcg aatatcgcct cccgcagtaa gctgtacact tcccagtccc
     5101 gccggtaatt ccagcggttg tagccacgtc tgccacggca caatggtttc gctttgcccg
     5161 gcaacggatt taatccacca cagttgcgat tgatagcggc ggatttcaaa cgcccccaaa
     5221 cgtaaacagg gggaagcatc ttctcgcgcc agcgccactt cctgccagat cctcaccaac
     5281 gcgtcgcggg aaggcatcgg tgcattctgc cctgccagcc agcggcggat aatcgccgcg
     5341 cgacgggcat cactcatcgc cagcattggc gcaatctgca gcgtcccctg cggcgtttga
     5401 cagtgtgcta aatcatctgc cagcagttca tccagcaggc tctcttgctc agcacaaagt
     5461 gcagcgctgc gggccgttgc ttcggcaaaa tgcggccaac gctgctgcaa taacggcact
     5521 acgcgcaggc gcagaaagtt acgatcgtat gagtcgtcct gattactttc gtcttcaatc
     5581 cagcgtaaac catgcgccaa tgcccactgc tccagttccc cccgcgtgcg ggcgagcaac
     5641 gggcggataa gctgtgttcc ggcaaactcc gaaacttccc ccatagccga aagcccggca
     5701 gggccgctac cgcgttttag cgccagcaga aaggtttcac attggtcgtc gagatgttgc
     5761 gcggtgacca gcacttcacc gggtaacagg gtacgagcaa atgcctgata acgcacctgc
     5821 cgcgcctggg cctcaatgcc cagtccttct tgcgcaagtt gcacgcgctc gaccaccagc
     5881 ggcacctgcc actgttggca gatgttctcg caatgcctaa cccaggcatc ggcattggca
     5941 cttaaaccgt gatgcacatg gatagcgcgt agagtgacgc ccggattttc cgtccgccac
     6001 tgcaccaact gatgcagcag aacggtggag tcaagcccgc cgctaaaggc caccagaatc
     6061 tggcgtgagg agagaagttg tctattgagc gtgagtgtca tgatgatgcg attttacagt
     6121 agcaatgccc ggcacgttac cgggcaaata tggcgatgac aagccttact gctcatacag
     6181 ttccaacggc agtccgtccg gatcgttaaa gaacgtgaag cgtttttgcg tgtagggatc
     6241 gacacggatg gcttcacact tcacgttatg gctttcaagg tacgccactg ccgcatcgat
     6301 atcatcaacg ctaaaagcca gatgacgcag gccgcaagct tccggtcggc tggggcgctc
     6361 cggcgggaac ggaaatgaga aaagctcaat cacatattgc ccattaagcg ccaaatcccc
     6421 tttccacgaa tcgcgcgctt cgcgatagac ttcgctttgc agcgtgaaac caagaatatc
     6481 gcagtaaaac gctttgctca ccgcataatc cgtcgcaata atcgcaatat ggtgaacctg
     6541 ttttaaaccc agcataacgt ctcctttatt tgttaacagc acgttactcg cccggaagcc
     6601 gctctggcaa gttatcccgc catttttagg actcgtacgc ggtaaatgcc gtcttcgtcc
     6661 tgtttcgcgc cgtgaatatc cgtttcaaaa ccggggtaat gttgccctac ggaacaaagc
     6721 atcagtagaa aatcgagtac cgtgcggctc tcttcggtca gcatttctcc aggcatcaac
     6781 agcggtacgc cgggtggata aggcaggatc atatttgccg atactctacc gaccagttgt
     6841 tccagcgcaa tggtttctac ttcaccttta atctgtctct gccatgcctg atgtggcgtc
     6901 atgatcatct ccggcaaggt atcaaatgcc cgcaacatca aaccgggaag atcgtgttta
     6961 cgaatcagct tatggatccc ttgcgccaga tcctgaatac gcatactgcg gtagaaatcg
     7021 ggatcttctg catagagatc gggcagcata tttttgatcc gcaggttgag atcgtaagag
     7081 cgcttaaatt ccgtcaaccc acgcaataat cccattgctt tggttttatc gatgccaata
     7141 ctaaagagaa acagcaggtt ataagggccg gttttctcta ctacgatccc acgttcgtcg
     7201 aggaattttg ccaccagcgc cgccgggatc ccctcctcgc tcatattgcc ctgctcgtcc
     7261 atccccggtg tcaaaatagt gactttaacc ggatcgagaa acatatgatc ggcatccgca
     7321 tcgttaaagc cgtgccactg ttcgccaggc gcaacgggcc agcattcggc ttcatccacc
     7381 tgcggcggtt gccagatatc gaaaaaccaa ccgtcagact cttcccgcag ccgctggacc
     7441 tctttgcgaa aatgcagcgc ccgctccacc gaacggttaa tcagccgttt gcccggatta
     7501 ccacgcagca tcgccgccgc cgtctcaacc gaagcaacaa tgggataact gggcgaggtg
     7561 gtggtatgca tcataaaggc ttcgttaaac gcctcttcgt catactcgcc tttaatgtgg
     7621 atcagcgaag cctgcgataa cgccgccagc attttgtggg tcgattgcgt ttcgaagatc
     7681 acttttcccg caacacgctc gccgctcata ccacttttac cctggtagat cggatgaaaa
     7741 tgggtgtacg gcacccaggc agaatcgaag tgaatcgacg ggacatccag cgtctgtttg
     7801 atccagtcgg tgttgtagag caagccatca taggtggagt tggtaatcac cgcatgaacc
     7861 ggccattgtg cttgcgtggt agcagcgact ttctcttcga tactgtcgcg agaaaattca
     7921 cggcgcggga tcccaccaag aatccccaac gcattacgtg ttggttttag ccagaccggc
     7981 actacatcgt tcatcatcaa cagatgcgcc agcgatttat gacaattgcg gtcaattaac
     8041 agcgtactgc cggatggcgc ggcgtacata cccacaattt tgttcgacgt cgatgttccg
     8101 ttggtgacga tataactctg ttccgcgcca aacgtccgcg cgatgtactc ttccgcttcc
     8161 aggtgtggcc cggtgtggtc gagcaacgaa ccaagctcgg tgaccgaaat agagacgtcc
     8221 gccttgaggg tattcccgcc gaaaaaatca taaaacagac agccaaccgg gcttttttga
     8281 tatgcggtgc cgcccatatg ccccggcgta caaaaggtgt acttccgctc tttgacgtag
     8341 gtaaacaagg ctttcgtgaa cggcggcgta atgttatcaa gatattcgtt ggtgtactga
     8401 cgcatacgaa tggcgatatc ttccgcctgc cccagcgcat attcaaaaaa ccagagcgcc
     8461 atccgcatat cctgcacgct gacatccatc gtcgagtggg tgttgatgaa ggcataaagc
     8521 gggagatatt cattaagctg attgatatcg ctacataaat cgagactgta ctcatcccag
     8581 tcaaaaatca cgccgcaaat tcgagggtta tgttcgataa atttcagcaa atcaacgctg
     8641 ttttgtggcc agataatctg aaagccttgc gccaccagcg ccgactccag ttctttgatg
     8701 ggctcatctt tataaaagac gccatgcggt cccataatgg caatgatgtt catgtgttcc
     8761 tcctggaaaa tccttcctta atcatagcct gctcaaaccg tggcgataaa aaagggccac
     8821 cgaagtgacc ctttttcaga acttttgcga attacgcgta accgtagctc atcaggcgct
     8881 gataacgacg attttttaaa tcttcagtgc ttaacacgtc gagatcggcc agatccgcca
     8941 gcagttgcgc tttcaacgat gccgccatcg cttccgggtt acgatgagcg ccgcccagcg
     9001 gttccgggat gatggagtcg atcagtttca gctctttcag gcgcggagcg atgataccca
     9061 tcgcttcagc cgccaacggc gctttgtcgg cgctcttcca cagaatggac gcgcaacctt
     9121 ccggcgagat aacggaatag gtgctgtatt gcagcatatt cactttatcg cccacgccaa
     9181 tcgccagcgc accaccagaa ccaccttcac cgataacggt acaaatagtc ggtacgctaa
     9241 ggcgagacat ttcacgcagg ttgcgtgcaa tggcttcaga ctgaccgcgc tcttctgcgc
     9301 ccacgcccgg ataagccccc ggggtgtcga taaaggtgat gataggcatt ttaaaacgtt
     9361 cagccatttg catcagacgc agtgctttgc ggtaaccttc tggcgctggc ataccaaagt
     9421 tacggcgaat tttttctttg gtttcacgac ctttttgatg accaatgatc atcaccggac
     9481 gaccatcgag acgggcgata ccaccgacga tagctttatc gtctgcatac gcgcggtcgc
     9541 cagccagttc gtcaaattca tcaaatgcca ggcgaacgta atccagggta taaggacgct
     9601 gtggatggcg tgccagttgc gcaatctgcc atgcaccgag atcggcgaag attttacgcg
     9661 tcagttctac gcttttttca cgcagacgat gcacttcttc atcgatgtta atatccagtt
     9721 tctcatcctg gcggctaacc gcagtcagag aatcgatttt cgcttccagc tctgcaatcg
     9781 gctgttcaaa atcaaggaaa ttcagactca tagtattcct gtattagtca aactccagtt
     9841 ccacctgctc cgaaccaatg aggccacgga gatcgtttaa taaacgatcg ctcggagaga
     9901 cacgccacgt cgcgccaaaa cgcaaccgcg cgcgtgcatc cgccctctga tagtagagat
     9961 gtactggaat tgtcccagag cggtggggtt ccagagactg acggagtcgg tttaaaagct
    10021 ggtcatcaat ttgcctgtcc gtcagcgaga tagcaagccc gcgagcatat ttttcccggg
    10081 cttcgtcaat atccatcact tcgcgagcgg tcattttaag cccaccgctg aagtcatcaa
    10141 agctgacctg tccgctgacg ataagtatgc ggtctttttc cagcaattgc tggtatttat
    10201 ccagggcgtc agtaaacaac atcacttcca gccgcccgga acggtcatcc agcgtgcaga
    10261 taccgatacg attgccgcgc ttggtgacca taacccgcgc agcaacaacg agccccgcag
    10321 ccgtgatgac tttaccacgt tctgtcgggt gcatgtcttt cagccttatg cctccgacat
    10381 aacgctcaat ctcttttaaa tactggttga tagggtgtcc cgtcaggtac agacctaacg
    10441 tttcacgttc cccatctaat accacctgct ccggccacgg ttggcagctg gcgtaggatt
    10501 gttcaatttg ttccggctct tctgccagca cgccgaacat atcggcctga ccgatggctt
    10561 ccgcttttgc gtgttgatca gccgctttta acgcatcgcc cagcgagttc atcaacgccg
    10621 cgcggtgtgg cccaagacgg tcaaacgccc cggacatgat caatttttcc agcactcgcc
    10681 ggtttaactt tttggtgtcg gttcgggcgc agagatcaaa cagttcgcgg aagtagccgc
    10741 ctttattacg ggcttcgatg atggcctcaa tcggaccttc accgacccct ttgatcgcgc
    10801 caataccata cacgatttcg ccgtcgtcgt tgacgtggaa atggtaaaga ccggagttta
    10861 tatctggtgg caggattttc agccccatcc gccagcactc atccaccagg cccaccacct
    10921 tctcggtgtt gtccatatcg gcggtcatta ccgccgccat aaactccgcc ggatagtgcg
    10981 ctttcagcca taacgtttga tatgacacca aagcataggc cgcagagtgc gatttgttaa
    11041 atccgtaacc cgcgaatttc tccaccaggt cgaagatttt catcgccagt tcggcgttaa
    11101 ttccgttctt ttctgcacct tcagcaaata cagaacgctg cttagccatc tcttccggct
    11161 ttttcttacc catcgcacga cgcagcatat ccgcgccacc gagggtataa ccagaaagca
    11221 cctgggcaat ctgcatgacc tgttcctgat acaggatgat gccgtaggtt ggctccagta
    11281 ccggtttcag gctttcatgc tgccactgca cgtccggata ggagatctct tcgcgaccat
    11341 gtttacggtc gataaagtta tccaccatcc ctgactgcaa cggaccaggg cggaacagtg
    11401 ccaccagtgc gatcatatct tcgaagcagt caggttgcag acgcttgatc aggtccttca
    11461 tgccgcgcga ttcaagctgg aataccgccg tggtttccga gcgttgcagc atgtcgaagc
    11521 ttttcttgtc atccagcggg atcgcggcga tatccagcgg cggctcgcca ttcttcgccc
    11581 gccgcttgtt gatcatctcc agcgcccagt tgatgatggt gagcgtacgc aaaccgagga
    11641 agtcgaactt caccagcccg gcgtattcaa cgtcgctttt atcaaactgg gtgaccggat
    11701 gtttgccctc ttcatcgcag taaagcggcg caaaatcggt aattttggtc ggtgcgataa
    11761 ccaccccacc ggcgtgctta ccggcgttac gggtgacccc ttccagtttg cgcgccatgt
    11821 cgatcagcgc cttaacttct tcatccgctt cgtagatttc cggcagctgc ggctcggctt
    11881 caaacgcttt cgccagcgtc atccccggat cgggcgggat cagtttcgag atacgatcga
    11941 caaagccgta cggatggccc agcacgcggc ctacgtcgcg gatcaccgct ttcgccgcca
    12001 tcgtaccgaa ggtgataatc tgcgataccg catcgcgacc gtacatgtcc gccacatgtt
    12061 cgataacctg gtcgcgtttc tccatacaga agtcaacgtc gaagtcaggc atggagacac
    12121 gttccgggtt aaggaaacgt tcgaacagca ggtcaaattc cagcggatcg aggtcggtga
    12181 ttttcagcgc gtaggccacc agtgaacccg caccggagcc acggcctggc cctaccggta
    12241 cgccgttatc tttcgaccac tggataaatt ccataacgat gaggaagtaa cccgggaagc
    12301 ccatctggtt gataacctga agttcagtct ccagacgctc gtcatattcc gggcggcgct
    12361 tcagacgttc ttcctcgtca gggaataaaa aggccagacg ctcttccagg ccttcttttg
    12421 cacgcttgac cagataatct tcggtgctca tgtccccggt cgggaattgc ggcaagaagt
    12481 attcacccag acgcacagtt acgttacaac gtttggctat ctcaacggtg ttggcaaggg
    12541 cttcgggaat gtcggcaaaa agctcgcaca tctcctcttc gctacgcata tattgctgcg
    12601 gcgaatagtt acgcgggcgt ttaggatcgt cgagggtaaa gccgtcgtgg atcgcgacgc
    12661 ggatttcgtg tgcgtcaaag tcgctgctgt cgataaagcg cacgtcgttg gtcgccacaa
    12721 cgggcaaacc gcgtgattcc gccagttcca ccgcagcgtg cagatagctt tcttcgtccg
    12781 gcctgccggt gcggatcagc tcgagaaaat agcgatccgg gaagtgttct tcataaaacg
    12841 cgacacactc atctaccagc gcgctgttac cacgcaaaag actgcgtcca acgtcgccca
    12901 tgcgcccgcc ggaaagaagg atcaaccctt catttaattc gataagccag tcgcgatcga
    12961 tgatcggccc ggcggcaccg tacccgcgct gatacgcttt tgagatcagc aacgtcagat
    13021 tctgatagcc ggtattgttc gccgccagca ccgtcaggtg ggttaactca tcacccagca
    13081 ggtcgcactg gacgttaaaa tccgccccga cgatgggctt aatccctgcg ccatgtcccg
    13141 ctccgtagaa cttcaccaga ccgcaaaggt tggtgaaatc ggtgatcgcc agtgctggca
    13201 tacccaacgc cgccgccttt tttaccagcg gtgcggtttt ggccaggcca tcgatcatcg
    13261 agtagtcgct gtgcacccgc aggtgtacga aacgtggttc agacatcttc agattccggt
    13321 ttacttaatc tcgacacaag aatcaggacg caagtcccag tgcgcgtttg acaggcccaa
    13381 agctgcgccg atggtgttcg gtcgcgccgt gttcagccag tttttccaga tgaaaagcgg
    13441 ttggataccc tttgtgttgg gcaaaaccat attgcgggaa aacaatatcc agcgccgcca
    13501 tttcggcgtc acgcgtcact ttcgccagga tagacgcggc actgatttcc ggtacgcggc
    13561 tatcgccttt caccacagcc atcgaaggca tcggtaattt cgggcagcgg ttaccatcaa
    13621 tcaacacata ttccggcgca atatgcagtc cagcgacggc acgctgcatc gccagcatgg
    13681 tcgcatgtaa aatattcagt tcgtcgattt cgtgaggttc cgcgcggccc agactccagc
    13741 tcaacgcttt ctctttgatc tcttcacaga gcgccagacg gcgtttttcg ctcagctttt
    13801 tggaatcatt cagcccggca attgggcgcg ccgggtcaag gattacagca gcggtaacca
    13861 ccgcgccaac caacggcccg cgtccaactt catccacacc cgcaaccagc tgcgtgtgtg
    13921 gataaacaaa ttcgatcatt gtgctaactc cagaacggct tgtgccgcct gctcatcggc
    13981 attgcagcgg atctgctgat gcagttcacg gaaggtatcg tgcatcgcgt gactggtttt
    14041 cccgttcgcc aacagcggta acagcgccgc agccagtttt tgcggctcac actcttcctg
    14101 caataactct ttgactaact ctctgcccgc cagcaaattt ggcagcgaga catactcggt
    14161 tttcaccagc cgcttcgcca gccagaaggt aaaaggcttc atgcgatatc ccaccaccat
    14221 cgggcatttc gccagcatgc actccagggc tgccgtaccc gacgccagta gcgccgcatc
    14281 actggcgacc atcgcctcac ggcccattcc atccagcaga tgaacagcaa ggtccggtgc
    14341 gacttcagct ttgatacgct caaactgctc gcggcgtttg gcattcacca gcggcaccac
    14401 gatctcgaga tcgggatatg tctgacgcaa aagctgggcc gttttcagga aatcggcact
    14461 aagcatttcg acttctgcac cacggctgcc cggcaacaat gccagacagt gggcatcgta
    14521 agggataccc agcacatcac gggcaccatt tttatctgga tctaatggca tggcatcagc
    14581 catggtatga ccgataaagc ggcacggtac gttgtatttg tcataaaacg ccttttcgaa
    14641 aggcagaaat gcgagcacca gatcggtggc tctgcctatt ttgaaaacgc gtttctgtcg
    14701 ccacgcccag acggacggac taacgtaatg aatggttttg acaccctgct ttttgaggtt
    14761 accttcaaga gtaatattga agtctggcgc atcaataccg acaaaaacat ctggcttcag
    14821 ttcgccaaaa cgctttgtca gatcggcacg aatatgcagt aagcgacgca gacgcccgag
    14881 cacttcaaca atgcccatca ccgccagctc ttccatttcg taccaggctt cgcagccttc
    14941 ggcttgcatt cgtggcccgg caacaccaac aaagcgggca ttgggcacac gttctttcag
    15001 agcacggatt aaaccggccc ccaggatatc gccggaggtt tctccggcga ccagggcaat
    15061 cgttaatgga cgctgttcag tcattaacga atcagaccgc gcgttgagcg tgcaaagaaa
    15121 tcggtaaagg ctttcacttc cggatatgtt tccgccagtt cagcaatttc cggtttcact
    15181 tcatcgagcg ttttaccgct acgataaatc agcttatacg cattgcggat agcggtaatc
    15241 gcctcacggc tgaatccgcg gcgcttcagc ccttcgatat tgacaccgaa cggcgttgcg
    15301 tggttaccct gcgcaatgac ataaggaggg acgtcctgcg ccacaccgga gcagccgcca
    15361 accatcacat gcgcaccaat gatgcagaac tgatggactg cggtcatgcc gccgatgatc
    15421 gcgaagtcgt caaccgatac gtgacccgcc agcgttgcgt tgttggcgag gatgcagcgg
    15481 ttacctaccg tacaatcgtg cgcaatgtgc gcattgatca tcagtaagtt gtcgctgccc
    15541 accttcgtca atccaccgcc ctggactgtg ccacgatgaa tggtgacgct ttcgcgaatg
    15601 cggttacgat cgccgatttc cacgcgggtc ggttcgccag catatttcag atcctggtta
    15661 acttcgccga tggaggcgaa ctgataaatc tcattatcgc ggccaatttt agtatgacca
    15721 ttcacgacaa cgtgagattt cagtacggta ccctcaccaa tttcgacatg gggtccaacg
    15781 atacaaaaag gaccaatgtg agcgttcgcg ccaatcgacg cgccctcttc cacaatggcg
    15841 gttggatgca caaaggcgga tttatcaatc acgtatcagg cctcccggct acgagcacac
    15901 atcatcgttg cttcgcaaac tactttaccg tcgaccagag caaccccttt aaaacgggtc
    15961 aggccgcggc gcgttttttc gaaagtgact tccatgatca tttgatcgcc aggcacgacc
    16021 gggcgcttaa agcgcgcttc gtcaataccg gcgaagtagt acagctcacc cggttccagt
    16081 tttcctacgc ttttaaacgc cagaatacct gttgcctgtg ccattgcttc cagaatcagc
    16141 acacccggga aaatcggttt tccagggaaa tggccctgga agaatggctc attgacagag
    16201 acatttttta ctgcgcgcag aaaacgacct tcttcaaaat ccagcacgcg atccaccagt
    16261 aagaacggga aacggtgcgg cagaagttct aaaatctctt caatctgcag agtatgagtg
    16321 ttagtagtca agatactctt cctgtcaaaa tataagaaac gacaataata acacggcctg
    16381 ccgcaatcgt aagaatgaga caggccgtaa agtttggcga acaaaagatg gaacgttagt
    16441 cttgttgatt aaccttgcgc tcaagcgatt tcagacgctt gctcatgtca tcaatgttca
    16501 tcaccagtgc agcggttttg cgccagactt tgttgggttg cagcggaatg cctgaggaat
    16561 agacgcctgg ttcagtgatg ggacgcatca ccatacccat gcccgtaacc gtcactttgt
    16621 cgcatatttc catatgcccg ttgattacgc tggctccgcc gatcatgcag taacgaccaa
    16681 ttttcaggct gcccgccata atgacgccac cggcaactgc cgtattgtcg ccaatcacga
    16741 cgttatgtgc aatctggcac tggttatcaa tgatcacacc attgccaata acagtgtcat
    16801 ccagcgcacc gcgatcgatg gtagtacatg cgccgatctc cacgcgatcg ccaataatta
    16861 cgcgacctat ctgcgggatc ttcacccagt taccacgatc gttggcataa ccaaagccgt
    16921 ctgcgcctac cactgttccg gactggatca ggcaattctg accgatctgg atctcatggt
    16981 aaatggttac gttcgcccag agacgcgaac ctgcaccgat tttgctgttt ttacctacga
    17041 agcaaccggc accgataatc acgttatcgc ccagttcaac gccggactca atcaccgcgt
    17101 tagcgccaat tgagacgttg ttacccagct tcgccgtcgc gtcaatcact gcactgggtg
    17161 caatgttctg cgcgggctgc ggcgtggtat ctaaaatttg cgccatgcgc gcgtaagtca
    17221 ggtagggatt cttcactacc agcgcggcac ttttcgcgaa aggaagatcg tcctgggtca
    17281 tgacaaccgc ggacgcctgg cacaagccta aatgctcacg gtattttggg ttaaccatga
    17341 acgtaatgtg acctgtttgt gcagattgca tggacgcaac gccggtgatg acgatatcgc
    17401 catcaccgtg tagttctgca tccaactgct gcgctaaatc agccagtcga attgaaggca
    17461 ttacttattt aacctgtttc agtacgtcag cagtgatgtc ttttacatcg ctgctgttgt
    17521 aagcaacggc gtttgcatca acaaccagat cgatatcctg gctgttggca acggatttca
    17581 cagcagtctg gatacgagta accagtttgc cacgttcttc gttggaacga cgagcgcgat
    17641 cctgctcaaa agcctgcgct ttctgagcaa aagtctggcg ctgagccatc acgtcttttt
    17701 ccagcttagt gcgatcgctg ccggctttca tggactgcag ctttttcatt ttagcttgca
    17761 gatcagtttc catacgctgc agttcgctgg cacggccttt gaactcattt tccagcgtat
    17821 tagaaacacc ggttttctgc gctacctgct gaaacaggct gcccatattg acgattgcaa
    17881 ttttgtcagc cgcctgagca gaagttgcca gtgctaaacc gagacctgcc gctaataacc
    17941 actttttcac aataaactcc ttaccatccc atttgcaccg gaaggtgcag ttctttgcgt
    18001 ggcccggcga tcttatcgag atcgcctaaa gtcatcgcta cactaccact acattccttt
    18061 gtggagaaca cttaccaggt tttaccaatg ttaaactgga actgttcagc cttgtctcca
    18121 tcgtactttt tgaacggctg ggcgtaggag aacaccaacg gccccaatgg ggacatccat
    18181 tgtaatgcga tacccgcaga catacggata ttgcttggat cactatagtc cggatatcca
    18241 gaatattggc tggaatccca gtttgtatcc caaacggtac ccatatccca gaagaaggag
    18301 atacggaccg agttggcata cttgtcgcta atgaacggcg tcggagtgat gaactcgagg
    18361 ctggcaaccg ccatagcgtt accacctaca gcatcatccg atttacataa atcttttgcc
    18421 ccatcctgcg tcgcacacgc atagtcatta cttgggtcat agccattagg gctatatggg
    18481 aagtaaaccg ctttcggacc aatggtgttg gactggaagc cacgcacggt gctggaacca
    18541 ccggcataga agttctcgta gaacggcatc tctttgccgc ctaaaccatc accataaccc
    18601 cagcgggtac gacccagaac aacccatttg tgatcgtcat cgatcggcac ataagtcgcc
    18661 gtgtctaacg tcactttgta gtattcgtta tccgatcccg gaatggtcac tttaccggtc
    18721 aggttgacac gtgaaccatc tgtcgggaag taaccacggt caagcttgtt aaaggtccaa
    18781 ccatagttga acgtgaagtc gtccgttttg aagctgttat cctgatcaga ggtgctcgga
    18841 tgttcaccca tagagtacag ataacgccac atcgcaacct gaggctgcat gttggacagg
    18901 gagttatgta cataacccag acctgcacgc agcgagttat attcgttaat cgggaagccc
    18961 aacgtcacgt ctgtaccata actcttgttg gtatagtcgg acaggtcggc gtcatctgcc
    19021 tggaagtcat tatagaagag acgaccaccg aggcttacac catctacggt gaagtacggg
    19081 ttggttaccg atagttcagc ataagtctgg taatcgtttt tggtcccgtt gataccaaca
    19141 gcataacccg tacctaacca gttatcctgt tgcacgccag cctggaagct cacgccactt
    19201 tcagtaccgt aaccaatccc gaagttgaag ctaccggtgt tacgttcttt caccttgtag
    19261 actacatcaa cctgatccgg gctacccgga acacgttggg tatcggtatc gacagtttca
    19321 aagaagccca gacggttcag acgctcctta ccctgatcga ccagatcgct ccccagccat
    19381 gcaccttcca tctgacgcat ttcgcgacgc aggacggcat ctttcgaggt atcgttacct
    19441 tcaaaacgga tcttacgcac atagaaacgg ttacccgcat caacgttcac gcgtaattta
    19501 acggttttgt cggcatcgtt aatttcaggc atcgactgta cgcgcggata ggcataacca
    19561 tagcgaccaa gaagcttttt aatgtcatct tccattttgg tcactttggt gccgttatac
    19621 agctcgcccg gctcgatctt agtcagctgc tcaatttcag cggagtgccc ggcaagattg
    19681 ccgctcactt caacgccaga aagcttgtac tgatcgcctt cggtgatgtt caccgtgacg
    19741 taaatacctt ttttatctgg cgtcagactg acctgggtag aatcgatgtt gaaacgggca
    19801 taaccgcgat ccagatagta actgcgcagg gtttcaaggt cgcctgccag tttctgtttc
    19861 tggtatttac gatcgcctac cacgttccac cacggcactt cgtcacgcag ttggaaatga
    19921 gagatcagtt catcggtggt gaaagcatgg ttaccaacaa tgttaatttg ctggatttca
    19981 gctgacacac cttcctggaa caccagtttt aggtcaacac ggttgcgcgg cagcggggtc
    20041 acgacagctt taacgctggc gctatattta ccgacgctgt agtagaagtc ttccagacct
    20101 ttctcgatat cggcaatggt ggtgcgatcg agggattcgc ccacacgcac accagaagcc
    20161 tcgaggtttt gcttcagcat gtcatctttc accgatttgt taccggagaa agtaatgctg
    20221 gcaatggtcg gacgttcttt tacctgaacc agaagggtat caccatcacg aaggacgcga
    20281 acatcctcaa agttgccggt agcaaacaga gcgcgaatgg tattactgat atcttcatca
    20341 ttaaccgtgt cgcctgtgcg caccggcata ctgaggaggg ccgcaccaac ggcgacacgc
    20401 tgaaggcctt cgaaatgaat atctttcact acgaaccctt cagcaccgta tacggtggcg
    20461 ctgctaaaca gcagcgacgc tatgagcaac tttttcatcg ccatcgttat tatgcgttct
    20521 tcctaactaa ctctcataac cgagagaaat cattgaaaag tgcaagcccc attaacagca
    20581 ccagcagaat cgagccaatg cgataacaaa agtcttgaac ccgctcggat accggtccgc
    20641 ccttgatctt ttcgatcgca aggaacagca gatgcccccc gtcaagtacg ggcaacggaa
    20701 acaggttaat tatccctaag ttcacgctaa taagcgcaag aaacggcagg taataaacaa
    20761 ccccgagttc cgctgtcatc ccagccccct tggcgataga gatcggccca ctgaggttgt
    20821 tcagtttcac atcaccggtg atcaattttc ccagcatact gaccgtcagc ttcatcagct
    20881 gccacgtttt gtccgtggct tcgacaatgg cgttgaacgg cccatactgg cgtacaactt
    20941 tatactcatc tggcaaagga atgactttcg gctcaatacc gacaaaacca atcgctttac
    21001 cattacccgg tttactctcc gggattaatg tcaaagacaa aggactcccc tgcctttcga
    21061 tttctaacgc taaggattta cccgggttat cccggacaag catcacaaag gtcacccact
    21121 gcgttaaggg ctgaccatcg actttaacga tcctgtcgcc tgcttgcaaa cctgccttgc
    21181 ttgccgccga gtttggctgc acattttcca gtacaggttc aatttgcggc ccacgaggac
    21241 gaatccccag cgaagttacc ggatcttctt tatcaggctc aaacgcccag tgacgtaaat
    21301 cgagctttac atcccgccgt tggtcgctgc caaatggcgc taccgtaatg gtggtgcttt
    21361 catcgccaat tttatcgacc aactgcaaac gcacggcatc ccaatcaggc gtttcgatac
    21421 catctacggc ttttagttcc gtacctggtg caatttgtgc ttccgcagct atcgaattgg
    21481 ctgctatttc accaaccacc ggacgtacgc caggcacacc aataataaaa accagccagt
    21541 aggcaaagat agcaaaaatg aagtttgcaa ccggacctgc ggcaataatc gccgctcgtt
    21601 ggccgacaga tttattattg aaggcatggt ggcggagttc cggaacgacc ggttctgcgc
    21661 gctcatccag cattttgaca taaccgccca acgggatcag ggcgataaca tattcggtgc
    21721 cgagcttatc agttcgccgc cagagcgcct taccaaaccc tattgagaaa cgctcaacgc
    21781 gaacaccaca acgccgggca acccagaaat gaccaaattc atgcacggtg ataagtacac
    21841 ccagtgcaac gatgaacgaa gccaaatccc agagaaaact cagcatatta ccttccgtta
    21901 aagcgtcctg aataccagta acaacaagca agcaaagacc ggtaccgcag ccgtcaggct
    21961 atcaatacga tctaaaatac caccgtgtcc tggaattaaa tgaccgctgt ccttaattcc
    22021 tgcttcacgc ttaaacatac tttcggtcag atcgccgagc actgaggcta acgctgcgac
    22081 aatagagcaa atgagtaagg tgacgggagc gacgtcgaga ttcacccaca tgccataacc
    22141 ccatgagatt accgctgcgg tcgcgagtcc accgataaag ccttgccagg ttttacccgg
    22201 agaaaccttc ggtgccagtt tatgtttacc aaacaatttg ccaaacatat atgcgccaga
    22261 gtcagcgccc cataccagga tcatgacata gagcagccat attgcgccac tgtaatgatt
    22321 ctcgtcgtag tgccaggccc gtaacgccag catgccccag aagaagggaa caatggttag
    22381 cacgccaaaa ataatgcgca atgttttaga gttacgccag attgctgcgg aacctgggta
    22441 aaacagcacc agcaatagcg cgacaatcca ccaacccagc gaagcccaaa gtgagatttc
    22501 aaccagcggt tgatgaatat ttcggtgata ttccggcaac agaaaaagca tcagcgccaa
    22561 caataacccg cataacaccg ccaaccatac tcgctgcgaa cgagtggtaa aaccgctaag
    22621 ctgtccccat tcccacgctg ccagcataca gaccaccagc gttacaatgg cgaaccccac
    22681 cggcggcaac agaaacaacg ccgcgatgac gacgggtatt aacacaaaag cagatatcag
    22741 gcgatacttc agcaaaagcg acccccatca ggctgtttca tcaccgggct cggtgccgcc
    22801 gaaacgacgc tctcgattag caaaggcatt taacgcccct tcaaagtctt gttcatcgaa
    22861 atcgggccag agaacatctg taaagtaaag ttcggcatag gcaatttgcc aaagcaaaaa
    22921 gttactaatg cgatgctccc ccccagtcct aattactaaa tctacagggg ccagttcatg
    22981 catacagaca tgctggttta gcatctcttc atctatctga tctggttgca ggtttccttg
    23041 ctgcaccttt tcagccagtt gcctgactcc ctggactata tcccaacgtc caccgtagtt
    23101 cgccgcaata ttcagcgtca gaccggtatt cccggctgtt agcgcttcag atttacgaat
    23161 acgttcttgc aaacgcgagt taaagcgact ggtatcgcca ataatacgca gacgcacgtt
    23221 atgtcggtgc agactcttta cttcgctatc aagcgcccac acaaacagtt ccattaacgc
    23281 actgacttcc tgcgctggtc ggttccagtt ttcactacta aaggcataca gagttaacgc
    23341 ctcaataccg ttgttggccg caaaagagac agcccggcgg acggattttg ccccggcttt
    23401 atgcccaaag gcacgaatct tcccttgctt ttttgcccag cgaccattgc cgtccataat
    23461 gatcgcaaca tgacgacagc catgcgctgg caatttttcg ctaagtggtt gagtagcaga
    23521 caacatcacg cgtttcttat tccctgacag ggtttacggt actcaggaat actgaagcct
    23581 atacaaaaaa aagccgtgta aaaccacggc atacctgacc ttataaagcc aactacttac
    23641 gatcagatgg cgcagactat atcactgaag ccctacgcta acaaatagcg cgactctctg
    23701 tagccggatt atcctcagct tgcgagacgc atcacctctt ttctggcgac ttcacgcgcg
    23761 ttcgcatcaa cagataacac atcgtccaca cattgtggtt cgcgcatatc cattttttcc
    23821 agtacggata aattcaacgc ggcgatatcc gtaaagcgga tttgttgcgc aagaaaagca
    23881 gcaacggtga tttcgtttgc ggcattcaat gctgtcgtcg ctgcctggcc ttgttcgaac
    23941 gcctccatcg ccagtttcag gcatggataa cgatcataat ccggtgcggc aaatgtcaac
    24001 gcacttagtt tgcaaaaatc gagcggcttc acgccagagt tcacgcgatt cggccatgcc
    24061 atggtgtggg caattggcgt acgcatatcc ggttccccca gctgcgccag aacactgccg
    24121 tcctgatagc gcaccattga gtgaatcact gactgcgggt gaatcagcac ttccatctgg
    24181 ctggcgctgg cgttaaacag ccaacgcgct tcaatgtatt ccagaccttt attcatcatg
    24241 gtagccgaat cgacagaaat tttacgcccc atcgaccagt tcggatgacg gcaggcttga
    24301 tccggcgtca ttgttgccaa atcgcgcaat ggcgtctcac ggaaagggcc accagacccg
    24361 gtaagtaaaa tggacaccac gccattttgc tcaaggtcag cgtatcccag attatgctgg
    24421 ataggttgcg gtaaactctg aaaaatggcg ttatgttcgc tatcgaccgg taacaattgc
    24481 gctttgctct gctttacggc gtccataaac agacgtccgc aggtaaccag tgattcttta
    24541 ttggccagca aaatggtttt acccgcgcgg atcgcagcaa gcgtaggtaa cagcccagca
    24601 gcgccaacaa tggctgccat cacctgatca acatcctcaa gcgctgccat atcgcaagcg
    24661 gcttgttgcc cacttaagac ttcggtgcgg ctaccctgtt gctgtagcat cgttttaaga
    24721 agtttcgcac tcgcttcatc gtccattacg gcatagcggg gagagaattc caggcactgt
    24781 tctaccatgc gagtgacatt tttgcctgcc accagcgcaa ctacgcggaa gtgttcggga
    24841 ttatggcgca ccacgtccag cgtgctgcaa ccaatcgagc cggtcgagcc cagaatggtg
    24901 agttgcttca tgaaacatcc agagttgaga cagaataaaa agcaaaacgc cgccagccga
    24961 tccgcaagga tctactgagc ggcgtttttg tcgttcaaga aatcagaact gcatcagttc
    25021 tgcttctttg tctgccagcg ccgcttcaat tttcttgatt gcagcatcag tcagtttctg
    25081 tacatcgtcc tgagaacggc gatcgtcgtc ttcgctgatc tctttatctt tcaacagtgc
    25141 tttcactttg tcgttcgcgt cacgacgcac gttacgtact gcaacacgcg cttgttctgc
    25201 ttcaccacga acgattttgg tcagatcttt acgacgttct tccgtcagcg gcggcagcgg
    25261 aacacggatg tcgctacccg cagagttcgg gttcaggcca agatcggacg ccataatcgc
    25321 tttttcaacg gccggagaca ttgaacgatc aaacacgttg attttcagag tacgggaatc
    25381 ttctaccgt
//
LOCUS       DAMZFT010000071        25306 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.071, whole genome shotgun sequence.
ACCESSION   DAMZFT010000071 DAMZFT010000000
VERSION     DAMZFT010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 25306)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25306)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25306
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.071"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(<1..1116)
                     /gene="selB"
                     /locus_tag="PN977_002935"
     CDS             complement(<1..1116)
                     /gene="selB"
                     /locus_tag="PN977_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HDI6042952.1"
                     /translation="MIIATAGHVDHGKTTLLQAITGVNADRLPEEKKRGMTIDLGYAY
                     WPQPDGRVPGFIDVPGHEKFLSNMLAGVGGIDHALLVVACDDGVMAQTREHLAILQLT
                     GNPMLTVALTKADRVDEARVDEVERQVKEVLREYGFAEAKLFITAATEGRGIDALREH
                     LLQLPEREHASQHSFRLAIDRAFTVKGAGLVVTGTALSGEVKVGDSIWLTGVNKPMRV
                     RALHAQNQPTETAHAGQRIALNIAGDAEKEQINRGDWLLADVPPEPFTRVIVELQTHT
                     PLTQWQPLHIHHAASHVTGRVSLLEDNLAELVFDTPLWLADNDRIVLRDISARNTLAG
                     ARVVMLNPPRRGKRKPEYLQWLASLARAQSDADALSVH"
     gene            complement(1113..2504)
                     /gene="selA"
                     /locus_tag="PN977_002936"
     CDS             complement(1113..2504)
                     /gene="selA"
                     /locus_tag="PN977_002936"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HDI6042953.1"
                     /translation="MTTETRSLYSQLPAIDRLLRDSSFLSLRDTYGHTRVVELLRQML
                     DEAREVIRGSQTLPAWCENWAQEVDARLTKEAQSALRPVINLTGTVLHTNLGRALQAE
                     AAVEAVTKAMRSPVTLEYDLDDAGRGHRDRALAQLLCRITGAEDACIVNNNAAAVLLM
                     LAATASGKEVVVSRGELVEIGGAFRIPDVMRQAGCTLHEVGTTNRTHANDYRQAVNEN
                     TALLMKVHTSNYSIQGFTKAIDEAELVALGKELDVPVVTDLGSGSLVDLSQYGLPKEP
                     MPQELIAAGVSLVSFSGDKLLGGPQAGIIVGKKEMIARLQSHPLKRALRADKMTLAAL
                     EATLRLYLHPEALSKKLPTLRLLTRSAEVIQIQAQRLQAPLAAHYGAEFAVQVMPCLS
                     QIGSGSLPVDRLPSAALTFTPHDGRGSHLESLAARWRELPVPVIGRIYDGRLWLDLRC
                     LEDEQRFLEMLLK"
     gene            complement(2602..3210)
                     /locus_tag="PN977_002937"
     CDS             complement(2602..3210)
                     /locus_tag="PN977_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HDI6042954.1"
                     /translation="MKLVGSYTSPFVRKLSILLLEKGITFEFINELPYNADNGVAQFN
                     PLGKVPVLVTEEGECWFDSAIIAEYIELMNVAPAMLPRDPLESLRVRKIEALADGIMD
                     AGLVSVREQARPAAQQSEDELLRQREKINRSLDVLEGYLVDGTLKTDTVNLATIAIAC
                     AVGYLNFRRVAPGWCVDRPHLVKLVENLFSRESFARTEPPKA"
     gene            complement(3367..7176)
                     /locus_tag="PN977_002938"
     CDS             complement(3367..7176)
                     /locus_tag="PN977_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_107086997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HDI6042955.1"
                     /translation="MNFVYTLKLNKKRELVVVSELSGGVKKSARNKLLKSVVVLMTTL
                     ATQLYSPLIQASIVGMDIPYQTYRDFAENKGAFSVGALDIPLYKKDGTLYSTLNKAPM
                     IDFSAVDSGQTVATLISPQYIVSVKHNTGYKNVRFGYRDDSSYILVDRNNSSVDFHTP
                     RLNKIVTEVVPADITDAGTANGTYQNQDRFPIFYRVGTGTQYVKDRNGKLTQLAGGYA
                     YRTGGTVGKPTSSNKRIVSNPGNTYSAANGPMPSYGIPGDSGSPLFAWDTQRNKWVLV
                     AVLNSYAGNAGKTNWFTVIPVNEVSANIEADTDAPVTPTSTTENINWTYDISTGTGKL
                     TQGTDAWEMHGRDTGSSAVSFNHGKDLSFENTGTVVLKDIVNQGAGTLTFNGDYIVKP
                     DADQTWVGGGIIVNGDHTVNWQVNGVKGDSMHKLGTGTLNISGTGINPGTLSVGDGTV
                     VLAQKPDSNGQVQAFESASIVSGRPTLVLSDSQQMNPDNIKWGYRGGKLDINGNDLTF
                     HALNAADEGAILTNSGSLATTSLDFNSTDTTKPVTTMFHGFFTGNVNVKNNATSNVNN
                     TFVVDGGINTPAGSMTQQGGRLFFQGHPVIHAVSTQSVANKLKALGDDSVLTQPVSFT
                     QSDWQTRQFNLKSLDLNNAAFYLARNAGLITTINANNSTVTLGSEDLYIDTNDGNGVK
                     TTPVEGQSVATASEDQSHFTGNVNLTNGSALRVNENFSGGIISSNSSVTISSTNANLT
                     ESSMFTHSVIKLSDNAQLTSTAGLQSDGTIEFGNGAKLSLLGESSSTFTPFSATAWNL
                     KGTGSSLNIGSGTNVNGDINAWSDTNINFGNSGKQSTSSGILYTGDIYAPEANVSIDN
                     TSWTLNKTSLLGNLTLKNSQLIMSTDGKTSSGIKVVDTFSGENNILYVKPTRSLSEMS
                     VSNIPLITAKNVTNNTRVFKTVTQQTGFHSMTPKIEVVNVDGTTQWRLKGFDVQSDST
                     ALKEGQRLMNTNIKNFLTEVNNLNRRMGDLRDTKGETGAWARLMNSSGSGYGGFSDRH
                     VHLQVGADRKHHFEGGDLFTGVMMTVTDSKASAESYQGKTRSVGGGLYASTLFDSGVY
                     VDVIGKYVHHSNDYLLQTMGLKADDTAHSWYLGAETGWRYQWKPDVFIEPQAELVYGT
                     LSGNTFNWQYNGMDVSMERKKAKPLIGRTGVEFGKTLDGRDWQVTAKAGLSYQFDLRN
                     TGATTYRDFAGESTVYNGKDGRMLANIGIDTRIKDNTRIGLTVEKSAFGKYNVDNAIN
                     ANIRYTF"
     gene            complement(7348..7866)
                     /locus_tag="PN977_002939"
     CDS             complement(7348..7866)
                     /locus_tag="PN977_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000610758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HDI6042956.1"
                     /translation="MINSFCKYILIAVTAQAPLWSNNALAAQTASANLPITMAVTNPQ
                     CTINNGQGLPATVQLPVVTTAGVFADNNSTEVPIVINCAGSVSKFEITLIGGTASKIT
                     TSNNMVDIALVWKQGGSAVQFGTPVALNKAPYLVSNKEFDASLIATVTPHTGNIPAGI
                     YTASLPITLTYY"
     gene            complement(7859..8380)
                     /locus_tag="PN977_002940"
     CDS             complement(7859..8380)
                     /locus_tag="PN977_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000521294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HDI6042957.1"
                     /translation="MGKWKILFCMIALLCNCCYASRTISNRVNITATMALNTCALTLT
                     PDNVNFGGVRIDEIDAASISPQTVSLAMQCSWPASGVSVKFVPKDGVSTSDPNIMKTG
                     LAGVGLALSWKKSTASDFTNINYNTSLQLNAQEQSSNVALGQFKLLPKKVPEQTIQSG
                     NIATSLTVEVSYD"
     gene            complement(8394..9365)
                     /locus_tag="PN977_002941"
     CDS             complement(8394..9365)
                     /locus_tag="PN977_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001170859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HDI6042958.1"
                     /translation="MQKLLFLVFTLFFISPHSHAAVDCSSGRITLGYDNTTTNYNTYN
                     STQNGPVATLVANVSQNFSCGGDYIETTTMKRKSYLVLPITSGGTCSNGVLTTTIPGV
                     VWRLDGFTCLGNGLKTTKELNGGDTWSGTVGKLILTLTDEYWRQNNSTRTFTLPAPSQ
                     GNVKGTNVNVVVTANPATLTHKVMHLGSCSMATTPVNLDFGTISPIEINKGALYQSVT
                     VTWNCVNKALAVNGFNLRFDPENVVNANQATFSATATDGKKLNFQLVQYNQGKETPPQ
                     LNKSIQVYAPVTNDVYNTLSLRIKVLPASTYPTGKVSTYLTITATYM"
     gene            complement(9395..10075)
                     /locus_tag="PN977_002942"
     CDS             complement(9395..10075)
                     /locus_tag="PN977_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012311580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
                     /protein_id="HDI6042959.1"
                     /translation="MKKINLMPVLALLLICFNSHAAFVLNSTRYIFAGDKDNISVQVN
                     NESSQEYGGQIWIDNANARDQNVYFAPSPTFFKVSGGHKQILRLLKINDSLPKDKESL
                     FWVNVQEIPKAPKEGTNSLAIALHTQVKMIYRPESLKEGREDAEKNIKIIHEGGNTIL
                     YNNSPYYFAVINVKQNGHDVKLSEETKNKIAAFAPFEKVSLGSKLSGKDISIVSFDDY
                     GVDREYKL"
     gene            complement(10083..12440)
                     /gene="pefC"
                     /locus_tag="PN977_002943"
     CDS             complement(10083..12440)
                     /gene="pefC"
                     /locus_tag="PN977_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PefC/AfrB family outer membrane usher protein"
                     /protein_id="HDI6042960.1"
                     /translation="MKKEIFIAAIIFHLLSKGALAEEFNYSFIRGGSKDIPDVLNSNK
                     ENVPGKYVVDVVFNGSKIASSTEMSIAKEDAEGICLSDEWLTENGIIINKDFYKNVYN
                     SARQCYLLGNEANSKVAFDQSLQEVSIDLPQAGFQDAAKDGGVWDYGSNGFKIAYDVN
                     TAKNSNQERTTYSSIDGQVNLGEWVLLGRGYAYQGENFDTNNLLLTRAIKSLKSDLQL
                     GKTQLYNSLNNGFTFYGAQLKSNQDMYPWNSRAYSPVINGIARTHARVTIEQSGYTLK
                     SIVVPPGPFVINDLNGVYSGDLIMKIYEEDGSVREQRFPVAVLPNLLRPGTYNYALAM
                     GSKVNQDNGERDKESLFAQMSYDYGFEPFTLNSSLLLDKNYNNIGLGLIRSFGWFGAM
                     SFSGNLSQAKYHNGKNLKGYSTSLKYAKALGDNANLQLIGYRFNSEDYIDYADFTYNS
                     YSFIRNRPKQRYESIVTYQLPEKGMFLNFSAWKEDYWDNYNEVGANLSLTKSFDQITM
                     TLNGGYSRLQNMDADYNVGLSLSVPLSLFDKTHYSFSNVNYDRRTGTSMNTGISGMIN
                     QRLSYNASVNQTRDTIGGTLSASYLFDWMQTSATYSQTGKNSSTSLQLGGSVIGVPEG
                     GIIFTPVKNDQLAIVQMKDVPGVMFNGSLPGDKYGRAVIPLTAYNNNTISVNAEKLPK
                     NIELTDNAINVTPTGNAIIYKNVKFKKINTYVVKLYGKNGYVVPMGSIAKNTQGKEVG
                     YVNNGGILLMNLETHDEGVISLDQCQFNTQSLKKNYDQTQEIHCE"
     gene            complement(12512..13039)
                     /locus_tag="PN977_002944"
     CDS             complement(12512..13039)
                     /locus_tag="PN977_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001361567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HDI6042961.1"
                     /translation="MKKLVLGSAILAVLGMTSTAMAANSGTVNFTGAVSSATCDLKVT
                     NASGAEIPTVDLGTVSTTGTPASVQFKLVPSDNACLAKNGAQISWTSNNLTTQGFSNA
                     TTGGTNAYMALLTSNAQETGASSYVKSGQTTFNYQVTSGGIRSFDYSASLVKPTGTVT
                     PGAFSTSASYVVAYK"
     gene            13784..14290
                     /locus_tag="PN977_002945"
     CDS             13784..14290
                     /locus_tag="PN977_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001054916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HDI6042962.1"
                     /translation="MNLVFILASDNNFFNYGMRLISDINKTFRCIDFTEIDDIVRADF
                     DTNEIYLICDIKNYYAYSLLLSGKSIKCIDTNNIRIHHNSIYIHKKKASVSEAINSLN
                     NIEMEILYLFYFHGKSVREISKMTNLSKEKIYYRVSRIKVKLGMKTTRKLPALLRIFF
                     NQTIEPED"
     gene            14481..15311
                     /locus_tag="PN977_002946"
     CDS             14481..15311
                     /locus_tag="PN977_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001088750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDI6042963.1"
                     /translation="MNSRVTCTKLDISVNRNLDKNHTLTLKKIRFYNSAMIFIRNAQL
                     VIKAKDGDVINIPPFSLCYIEKNLVVDATLKVLGSGIPYDIYTIDSYLLNSVCKVMEP
                     LLLGHQLVSQIRKKIFFYILEETDKEIFKRLINNNLPKHRQVYKIAYLLSRIHEIEPL
                     VYSLSVSTDITFTEKIKKIIESDLSRNWKLMDLRDILHMSEVSIRKKLEKENNNFNAL
                     VLDIRMHHAAKMITSTEKHINSIAYDVGYTSTSYFIRNFKSYFGITPKQFSLKVKRNN
                     "
     gene            complement(15467..16603)
                     /locus_tag="PN977_002947"
     CDS             complement(15467..16603)
                     /locus_tag="PN977_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HDI6042964.1"
                     /translation="MDLLIVLTYVALAWAVFKIFRIPVNQWTLATAALGGVFLVSGLI
                     LLMNYNHPYTFTAQKAVIAIPITPQVTGIVTEVTDKNNQLIQKGEVLFKLDPVRYQAR
                     VDRLQADLMTATHNIKTLRAQLTEAQANTTQVSAERDRLFKNYQRYLKGSQAAVNPFS
                     ERDIDDARQNFLAQDALVKGSVAEQAQIQSQLDSMVNGEQSQIVSLRAQLTEAKYNLE
                     QTVIRAPSNGYVTQVLIRPGTYAAALPLRPVMVFIPEQKRQIVAQFRQNSLLRLKPGD
                     DAEVVFNALPGQVFHGKLTSILPVVPGGSYQAQGVLQSLTVVPGTDGVLGTIELDPND
                     DIDALPDGIYAQVAVYSDHFSHVSVMRKVLLRMTSWMHYLYLDH"
     gene            complement(16606..16968)
                     /locus_tag="PN977_002948"
     CDS             complement(16606..16968)
                     /locus_tag="PN977_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HDI6042965.1"
                     /translation="MFLNYFALGVLIFVFLVIFYGIIAIHDIPYLIAKKRNHPHADAI
                     HTAGWVSLFTLHVIWPFLWIWATLYQPERGWGMQSHVASQEKATDPEIAALSDRISRL
                     EHQLAAEKKTDYSTFPEI"
     gene            17504..19417
                     /gene="mtlA"
                     /locus_tag="PN977_002949"
     CDS             17504..19417
                     /gene="mtlA"
                     /locus_tag="PN977_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HDI6042966.1"
                     /translation="MSSDIKIKVQSFGRFLSNMVMPNIGAFIAWGIITALFIPTGWLP
                     NETLAKLVGPMITYLLPLLIGYTGGKLVGGERGGVVGAITTMGVIVGADMPMFLGSMI
                     AGPLGGWCIKHFDRWVDGKIKSGFEMLVNNFSAGIIGMILAILAFLGIGPVVEALSKM
                     LAAGVNFMVVHDMLPLASIFVEPAKILFLNNAINHGIFSPLGIQQSHELGKSIFFLIE
                     ANPGPGMGVLLAYMFFGRGSAKQSAGGAAIIHFLGGIHEIYFPYVLMNPRLILAVILG
                     GMTGVFTLTILGGGLVSPASPGSILAVLAMTPKGAYFANIAGVCAAMAVSFVVSAILL
                     KTSKVKEEDDIEAATRRMQDMKAESKGASPLSAGDVTNDLSHVRKIIVACDAGMGSSA
                     MGAGVLRKKIQDAGLSQISVTNSAINNLPPDVDLVITHRDLTERAMRQVPQAQHISLT
                     NFLDSGLYTSLTERLVAAQRHTENEVKVKDSLKDSFDDSSANLFKLGAENIFLGRKAA
                     TKEEAIRFAGEQLVKGGYVEPEYVQAMLDREKLTPTYLGESIAVPHGTVEAKDRVLKT
                     GVVFCQYPEGVRFGEEEDDIARLVIGIAARNNEHIQVITSLTNALDDETVIERLAHTT
                     SVDEVLELLAGRK"
     gene            19512..20660
                     /gene="mtlD"
                     /locus_tag="PN977_002950"
     CDS             19512..20660
                     /gene="mtlD"
                     /locus_tag="PN977_002950"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HDI6042967.1"
                     /translation="MKALHFGAGNIGRGFIGKLLADAGIQLTFADVNQVVLDALNARH
                     SYQVHVVGETEQVDTVSGVNAVSSIGDDVVDLIAQVDLVTTAVGPVVLERIAPAIAKG
                     LVKRKEQGNESPLNIIACENMVRGTTQLKGHVMNALPEDAKAWVEEHVGFVDSAVDRI
                     VPPSASATNDPLEVTVETFSEWIVDKTQFKGALPNIPGMELTDNLMAFVERKLFTLNT
                     GHAITAYLGKLAGHQTIRDAILDEKIRAVVKGAMEESGAVLIKRYGFDADKHAAYIQK
                     ILGRFENPYLKDDVERVGRQPLRKLSAGDRLIKPLLGTLEYGLPHKNLIEGIAAAMHF
                     RSEDDPQAQELAALIADKGPQAALAQISGLDANSEVVSEAVTAYKAMQ"
     gene            20660..21247
                     /gene="mtlR"
                     /locus_tag="PN977_002951"
     CDS             20660..21247
                     /gene="mtlR"
                     /locus_tag="PN977_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol operon repressor MtlR"
                     /protein_id="HDI6042968.1"
                     /translation="MVDQAQDTLRPNNRLSDMQATMEQTQAFENRVLERLNAGKTVRS
                     FLITAVELLTEAVNLLVLQVFRKDDYAVKYAVEPLLDGDGPLGDLSVRLKLIYGLGVI
                     NRQEYEDAELLMALREELNHDGNEYAFTDDEILGPFGELHCVAALPPPPQFEPADSSL
                     YAMQIQRYQQAVRSTMVLSLTELISKISLKKAFQK"
     gene            complement(21258..21467)
                     /locus_tag="PN977_002952"
     CDS             complement(21258..21467)
                     /locus_tag="PN977_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6042969.1"
                     /translation="MGKLGENVPLLIDKAVDFMASSQAFREYLKKLPPRNAIPSGIPD
                     ESVPLYLQRLEYYRRLYRPKQVEEK"
     gene            21751..22113
                     /locus_tag="PN977_002953"
     CDS             21751..22113
                     /locus_tag="PN977_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibL family ribosome-associated protein"
                     /protein_id="HDI6042970.1"
                     /translation="MKEVEKNEIKRLSDRLDAIRHQQADLSLVEAADKYAELEKEKAT
                     LEAEIARLREVHSQKLSKEAQKLMKMPFQRAITKKEQADMGKLKKSVRGLVVVHPMTA
                     LGREMGLQEMTGFSKTAF"
     gene            22671..23354
                     /locus_tag="PN977_002954"
     CDS             22671..23354
                     /locus_tag="PN977_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HDI6042971.1"
                     /translation="MNLNKTLLSVLMILPLCLLAGCDYIEKATKVDDLVTQQELQKSK
                     IEALEKQQELDKRKIEYFEKQQTTIINSTKTLAGVVKAVKDKQDEFVFTEFNPAQTQY
                     FILNNGSVGLAGRVLAIDAVENGSIIRISLVNLLSVPVSNIGFHATWGNERPTDAKAL
                     AKWQQLLFNTTLNSTLQLMPGQWQDINLTLKGVSPNNLKYLKLSINMANLQFDTVQSA
                     ETRQRKNKK"
     gene            23398..>25306
                     /locus_tag="PN977_002955"
     CDS             23398..>25306
                     /locus_tag="PN977_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin"
                     /protein_id="HDI6042972.1"
                     /translation="MNKIFKVIWNPATGSYSVASETAKSRGKKSGRSKLLISALVAGG
                     LLSSSGALAQAGLDTGTGVTPAGHNNGTGWIAIGTDAEASTHTTTNGAATAVGYYSKA
                     LGMWSTALGAYSESNGNASLALGVKAQSNGDRSISMGASSSASINAGYSIAMGVFAFT
                     DAEYAVALGNESKALGKYSLALGNASQASGESSIALGNTSEASEQNAIALGQGSIASK
                     VNSIALGSNSSSAGENAIALGEGSAAGGSNSLAFGSQSRASGNDSVALGVGATAATDN
                     SVAIGAGSTTDASNTVSVGNSTTKRKIVNMAAGAISNTSTDAINGSQLYTISDSVAKR
                     LGGGATVGSDGTVTALSYALKSGTYNNVGDALSGIDNNTLQWNRTAGAFTAAHGSNTT
                     SKITNVAKGTVSATSTDVVNGSQLYDLQQDALLWNGTAFSAAHGTDVTSKITNVTAGE
                     LSDTSTDAVNGSQLKATKDDVAANTTNITNLTSEVAGNTTSITNLTDTVTNLGEDALK
                     WDDAAGAFTAAHGTNATNKITNVTAGELSDTSTDAVNGSQLKTTNDNVATNTTNIATN
                     TTNITNLTNAVDSLGDDSLLWNKAAGAFSAAHGTDATSKITNVTAGDLTAGSTDAVNG
                     SQLKTTNDNVAT"
BASE COUNT         6991 a         5963 c         5473 g         6879 t
ORIGIN      
        1 atgaacagat aacgcatcgg catcgctctg cgcccgtgca agagacgcca gccattgcag
       61 atattccggc ttacgcttac cgcgacgcgg cgggttaagc atcaccacac gcgcccccgc
      121 cagcgtgttg cgggccgaga tatcgcgcag cactatgcga tcgttatccg ccagccacag
      181 cggcgtgtcg aacaccagct cggcaaggtt atcttccagc agcgaaacgc gtcccgtaac
      241 gtggctggcg gcgtggtgaa tatgcagcgg ctgccactgg gtcagcggtg tatgggtttg
      301 aagctcgaca atcacccgtg tgaacggctc cggcggcaca tcggcaagca gccagtcgcc
      361 acggttaatc tgctcttttt ccgcatcacc cgcgatgtta agcgcgatac gctgcccggc
      421 atgggcagtt tctgttggct gattttgcgc atgtagcgca cgcacgcgca tcggtttatt
      481 tacaccagtc agccagattg aatcgcctac cttcacttcc ccgcttaacg ccgtaccggt
      541 aacgaccagc ccggcacctt ttacggtaaa tgcgcggtcg atcgcgaggc ggaaactatg
      601 ttggctggcg tgttcgcgtt ctggtaactg aagcagatgc tcgcgcaggg catcaattcc
      661 ccgaccttcg gttgctgcgg tgataaacag ttttgcctca gcaaaaccgt attcccgcag
      721 cacctccttt acctggcgtt caacctcatc aacacgcgct tcgtccacgc gatcggcttt
      781 ggtcagcgcc actgtcagca tcgggttacc ggtcagctgc aaaatcgcca gatgctcacg
      841 ggtctgtgcc atcacgccgt catcgcacgc caccaccaac agcgcgtgat caataccacc
      901 aacgcccgcc agcatgttgg aaagaaactt ttcatgaccg ggtacgtcga taaaaccagg
      961 cacgcgacca tccggctgcg gccagtaggc atagccgaga tcgatggtca tgccgcgctt
     1021 tttttcttcc ggcagacggt cggcatttac gcccgtaatc gcctgcaaca aggttgtttt
     1081 gccgtggtca acgtgtccgg cagtcgcaat aatcatttca acaacatctc caaaaaccgt
     1141 tgctcatctt caaggcagcg taaatccagc cacaatcgtc cgtcataaat acgaccaatc
     1201 accggcactg gcaattcacg ccagcgggcg gctaatgact caaggtggct accgcgtcca
     1261 tcatggggtg taaacgttaa tgccgcgctc ggcaggcgat caaccggcag cgaaccactg
     1321 ccaatctgcg aaagacatgg cataacctgt accgcaaact ccgcgccgta atgtgcggca
     1381 aggggagcct gtaaacgttg tgcctggatt tgaatgacct ctgcgctgcg ggtaagcagg
     1441 cgcagggtcg gtaatttttt actcagagct tcagggtgta aataaagacg caacgtggct
     1501 tccagcgccg caagggtcat tttatccgcg cgtaatgcac gcttcagcgg gtggctttgc
     1561 agtcgggcga tcatctcttt tttaccaaca ataattcctg cctgcggccc gcctaacaac
     1621 ttgtcgccgg agaagctcac cagactgacg cccgccgcaa tcaactcctg cggcattggc
     1681 tctttcggca aaccgtactg gctaagatcg accagcgagc cactgcctaa atcagtcact
     1741 acggggacat ccagctcttt gccgagcgcc accagttccg cttcatctat cgctttggtg
     1801 aatccctgaa tgctgtagtt actggtatgt actttcatca acagcgcggt attttcattc
     1861 accgcctggc gataatcatt agcgtgcgtg cggttggtgg tccccacttc gtgtagggtg
     1921 cagcctgcct gacgcatgac atcaggaata cgaaacgcgc cgccaatctc caccagttcg
     1981 ccgcgagaaa ccaccacctc tttcccgctg gcagtggccg ccaacatcaa taacaccgcc
     2041 gccgcattgt tattaacgat acaggcatct tccgcccccg taatacggca cagcagctgc
     2101 gccagcgccc gatcgcgatg tccgcgtccg gcgtcgtcca gatcgtactc gagggtcact
     2161 ggcgaacgca tagccttcgt aacggcttcc accgcggctt ccgcctgtaa agctcgccca
     2221 aggttggtat gcagcacggt tcccgtcagg ttgatcaccg gacgcagcgc gctttgcgcc
     2281 tctttcgtca accgggcatc aacttcttgc gcccagtttt cacaccatgc gggcagcgtc
     2341 tggctgccac gaatcacttc tcgcgcttcg tcgagcatct gacgcaacaa ttccaccacg
     2401 cgggtgtgac cataagtatc acgcaaagaa aggaaggagc tatcgcgcaa taagcgatca
     2461 atagccggaa gttgactata gagggaacgc gtttcggttg tcataggaaa cctggctgat
     2521 caaggccctc tcacacggag aagggcgttt aacataacca cggattgtaa cgtgagatgg
     2581 gtcaggagga catatcgcgc atcaagcctt tggcggttcg gtgcgagcaa aactttcgcg
     2641 gctaaagagg ttttcgacca gtttgactaa gtgtgggcgg tcgacacacc agcctggcgc
     2701 aacgcggcgg aaattgagat atccaacggc acaggcaatg gcgatagtcg ccagattgac
     2761 cgtatcggtt ttgagcgtgc catcgaccag atatccttcc agcacatcca gactgcggtt
     2821 gattttctcc cgctggcgta acaattcatc ttcagactgc tgcgccgccg gacgcgcctg
     2881 ttcacgcacc gataccagcc cggcatccat aatgccatcc gccagtgcct caattttgcg
     2941 cactcgcaac gactccagcg gatcgcgcgg caacatcgcc ggagcgacat tcattaattc
     3001 aatatattca gcgatgatcg ccgaatcaaa ccaacattca ccctcttcgg tcaccagcac
     3061 cggcactttc cctaacgggt taaattgcgc cacgccgttg tccgcgttat agggcagttc
     3121 attaataaat tcgaaagtta tgcccttttc taacaacaga atagaaagtt tgcgtacaaa
     3181 cgggctggtg tagctaccga cgagtttcat tatgtttcct ttttgttaac aaaatatgag
     3241 tatgacccat ggttacaaat aagcaggtgt tattgatatg agtgtgatga caaacgcaag
     3301 atggcccgat acgtacgcat atcgggcctt gtagatgaaa cgacctttcg ggtgagattc
     3361 taaatattag aatgtatagc ggatattagc attgatcgca ttatcgacgt tgtacttacc
     3421 aaatgctgat ttctcaacgg tcaggccgat acgggtattg tctttaatgc gtgtatcaat
     3481 cccaatattg gctaacatac gaccatcttt accgttgtac acggtggact caccagcaaa
     3541 atcacggtac gtggtagcac cagtattacg caggtcaaat tgataactta aaccagcctt
     3601 cgctgtaacc tgccagtcgc ggccatccag tgtttttcca aactccacac cagtacggcc
     3661 aatcaagggt tttgcctttt tacgctccat gctgacatcc ataccgttgt attgccagtt
     3721 aaaggtattg cctgacaacg tcccgtaaac cagttcggcc tgcggttcaa taaagacatc
     3781 aggcttccac tgatagcgcc aacccgtttc tgcgcctaaa taccatgagt gagcagtatc
     3841 gtctgccttt aatcccatcg tttgcagtaa atagtcgttg ctgtgatgga catatttacc
     3901 gattacgtca acatacacgc ctgaatcaaa caatgtcgat gcgtacagtc caccacctac
     3961 ggaacgggtt ttcccctgat aactttcagc gctggcttta ctatcggtaa cggtcatcat
     4021 gacgcctgta aaaagatctc ccccctcaaa atgatgcttt ctgtcagcac caacctgcag
     4081 atgtacgtgg cgatcggaaa aaccgccata acctgagcca gaagagttca tcaaccgagc
     4141 ccatgcgccg gtttcacctt ttgtatcgcg caggtcaccc atacgacggt ttaagttatt
     4201 aacctcagtc agaaagtttt taatattagt gttcatcaaa cgctgacctt ctttaagcgc
     4261 tgtactatca ctctgaacat cgaatccttt cagacgccac tgtgtagtcc catcgacatt
     4321 aaccacctca atctttggag tcattgagtg aaagcctgtt tgttgggtca ccgttttaaa
     4381 tacccgggta ttgttggtta cgtttttggc ggtaataagc ggaatattgc tgaccgacat
     4441 ttcactaagc gaccgggtcg gtttcacata cagaatattg ttttctccac taaacgtatc
     4501 aacaaccttg atgccgctgc tggtcttgcc atctgtagac ataataagct gactgttttt
     4561 gagtgtcaga ttgcccagca atgaggtttt gttcaatgtc catgaagtgt tatcaatgct
     4621 gacattagct tctggtgcgt agatatcgcc tgtatacaaa atgcctgatg acgtactttg
     4681 ttttccgcta ttcccaaagt taatgttggt atcagaccat gcattaatgt cgccattgac
     4741 gttggtgcca gaaccaatat tcagcgagga tccggtcccc ttgagattcc aggccgttgc
     4801 tgaaaaaggc gtaaaggttg acgatgattc ccctaacaat gagagttttg ccccatttcc
     4861 gaactcgata gtaccatccg attgcaaacc ggcagtgctt gtcagttgag cattatcaga
     4921 gagtttgatt acagagtggg taaacatgct gctctcggtc agattcgcgt tagtcgaaga
     4981 gatagttaca ctactattgc tactgataat tccaccactg aagttctcat tgacccgcag
     5041 ggctgagcca tttgtcagat taacgttccc cgtaaagtgg ctttgatctt ctgatgcagt
     5101 cgcaactgat tgaccttcca caggtgtcgt ttttacgcca ttaccatcat tggtatctat
     5161 ataaagatct tcactaccca aagtaacagt ggaattattg gcattgattg ttgtgattaa
     5221 tcctgcatta cgagcaaggt aaaatgccgc attgttgaga tccagcgact tcagattaaa
     5281 ttgccgtgtt tgccagtcac tttgcgtgaa agagacaggt tgggtgagca cggaatcatc
     5341 gcccagcgcc tttaatttat tggcgactga ctgagtgctc acagcatgga taaccggatg
     5401 tccctgaaaa aagaggcgcc ccccctgctg cgtcatacta cccgctggcg tattaattcc
     5461 gccatcaacc acgaatgtat tattcacatt ggatgttgcg ttatttttaa cattcacatt
     5521 gccagtgaaa aaaccatgga acatcgtcgt caccggcttc gtggtgtctg ttgaattaaa
     5581 atccaggcta gttgtcgcaa gcgaaccgct attggttaat atcgctcctt catcagctgc
     5641 atttaacgca tgaaaggtta aatcattacc gttaatatcg agtttgccgc cacgataccc
     5701 ccatttgata ttatccgggt tcatttgctg actatcgctc agtaccagag ttggcctacc
     5761 actaacaata ctggcggact cgaacgcctg aacctgaccg ttgctatctg gtttttgggc
     5821 gagcacaact gttccatcac ccacactgag cgttccggga ttgatgccag tgccggaaat
     5881 gtttaacgtc ccggtaccta gcttatgcat actatcgccc ttaacaccgt taacttgcca
     5941 gttcacggta tgatcaccat tgacaataat accgccccct acccaagtct gatcagcgtc
     6001 cggttttact atgtaatcac cgttaaatgt gagcgtaccc gctccctgat tgacaatatc
     6061 ctttaatacc actgtgccgg tgttttcgaa ggacaaatcc tttccatgat taaatgaaac
     6121 agcactactt ccagtgtcac gaccatgcat ctcccaggca tccgttccct gggtcagctt
     6181 accggtaccc gtggaaatat cgtaagtcca gttaatattt tcggttgtac tcgttggcgt
     6241 cactggcgcg tcagtatcag cctcaatatt ggcactcact tcattcactg gaataacggt
     6301 aaaccagttg gtttttcctg cattacccgc ataggaattg agtactgcaa ccaacaccca
     6361 tttattacgt tgcgtatccc aggcaaaaag gggagagcca ctatcaccgg ggattccata
     6421 ggaaggcata gggccgttag ccgcagaata ggtattacca gggttggaca ctattctctt
     6481 gtttgatgat gttggtttac caacggtccc gcccgttcta tatgcgtaac cgccagcaag
     6541 ttgagttaat ttcccattac ggtctttcac atattgcgta ccagtaccaa cacggtaaaa
     6601 gatcggaaaa cggtcttgat tttgataagt tccgttggca gtaccggcat cggttatatc
     6661 agccggaacc acttcggtta caattttatt taaacgcgga gtatggaaat ctacagaact
     6721 attattacga tcgacaagaa tataagaact atcatctctg tagccaaacc gaacattctt
     6781 atagccagta ttatgcttta cgcttacaat atattgtggc gaaattaacg tagctactgt
     6841 ctgtccacta tcaacagcgc taaaatctat cattggcgcc ttattcagtg tggagtacaa
     6901 cgtcccgtct ttcttataca aaggaatatc cagcgctccg actgagaacg ctcctttatt
     6961 ttcagcgaaa tcacgatagg tctgatatgg gatatccatt cccacaattg acgcctgaat
     7021 taatggtgaa tagagttgag tagctaaagt agtcattaaa acaacaacag attttaatag
     7081 cttatttcga gctgattttt taaccccacc agaaagttca gatactacaa ccaactctct
     7141 ttttttattt aactttagag tatatacaaa attcattgtc aatatcgcta taatctaatt
     7201 tttttacgat gaaattttaa cttaatgtat ttaataattc gttttagatg tctggcgcgc
     7261 tattaagagt gatttaacca gacattaata ccaatgaaat aaccccagca ttaatacgga
     7321 ccaattgata acttcctcaa tactttatta atagtacgtt aacgttatag gtaaactcgc
     7381 agtatagata cccgctggta tatttcctgt atgtggtgtg acggtggcta ttagcgaagc
     7441 atcaaattcc ttattactta caagatatgg tgctttattt aaagctactg gtgttccaaa
     7501 ctgaacagct gaacctccct gtttccatac aagtgcaata tcaaccatat tattactggt
     7561 cgtgattttc gaagcagttc ctcctattaa tgtaatttca aatttcgaga cactccccgc
     7621 gcagttaata acaataggaa cttcggtact attgttatcc gcaaatactc ccgcggttgt
     7681 gacaacagga agctgaacgg tggcaggtaa tccttgccca ttattaatgg tacattgtgg
     7741 atttgttact gccatcgtaa tcggtaggtt tgcacttgcc gtttgtgcag caagtgcatt
     7801 attactccag agaggagcct gcgcagtcac tgctataaga atatatttac agaaagagtt
     7861 aatcatagct aacctcgaca gttagactgg ttgctatatt gccactttgt attgtttgct
     7921 caggaacctt ttttggtaaa agtttaaact gccctaatgc cacattactt gattgttcct
     7981 gagcattgag ttgcaacgac gtattgtaat taatattggt aaagtcagaa gctgtactct
     8041 ttttccacga taaagccagg ccaacaccag ctagtcctgt tttcatgata tttgggtcac
     8101 tagtggaaac tccatccttt ggtacaaact ttaccgatac cccgctagca ggccaactac
     8161 attgcattgc aagcgataca gtctgcggag aaatactcgc cgcgtcaatc tcatcaatac
     8221 gtactccacc aaaattcacg ttgtctggtg ttaacgttaa tgcacaggtg ttgagagcca
     8281 tcgtcgccgt gatgtttact ctattcgaaa tagttcgcga cgcatagcag caattgcaaa
     8341 gtaatgcaat catgcaaaat aatattttcc actttcccat aaaatatcac cagttacata
     8401 tatgtcgctg taattgttaa atacgttgat actttccctg tgggataagt tgatgctggc
     8461 aatactttta ttcttaaact aagtgtattg taaacatcat tcgttactgg agcatacacc
     8521 tgaatgcttt tattaagctg gggaggtgtt tctttccctt gattatattg aaccaactgg
     8581 aaatttagtt ttttaccatc agtagcagtg gcactaaaag tggcttggtt agcattaaca
     8641 acattctcag gatcaaaacg taaattaaaa ccattcactg cgagtgcttt gttaacgcag
     8701 ttccaggtaa cagtaacaga ttgatataga gctcctttat taatttcaat gggtgatata
     8761 gtcccgaagt ccaaattaac tggtgttgtt gccatcgagc aactacctaa gtgcataact
     8821 ttatgcgtca aggttgccgg atttgctgtt accacgacat taacattcgt tcccttgaca
     8881 tttccttgag aaggggccgg taaagtaaat gttcgtgtgg aattattctg ccgccaatac
     8941 tcatctgtca gtgttaagat aagtttccct actgtcccag accaggtatc accgccgttt
     9001 aattctttag tggtctttaa cccattcccc aaacaggtaa atccatcaag gcgccagaca
     9061 acgccgggaa tggtagtagt taaaacacca ttactacaag ttcccccact ggtaataggt
     9121 aagactaaat aacttttcct tttcatagtg gttgtttcga tataatcacc accgcacgaa
     9181 aaattctgcg atacattagc aactaatgta gcaacgggtc cattctgcgt agagttgtaa
     9241 gtattgtaat ttgtagtcgt attatcatat ccaagagtaa ttctaccact ggagcaatcc
     9301 accgccgcat gactatgtgg cgaaataaaa aatagcgtaa atacaagaaa taataatttc
     9361 tgcatagcat tctccttaat aataaaggtt tgggctagag tttatattcc cggtctacgc
     9421 cataatcatc aaaagaaaca attgaaatat cttttcctga aagtttactt cccagagata
     9481 ccttctcaaa aggcgcaaaa gccgcgatct tatttttagt ttcttcgcta agtttcacat
     9541 catgaccatt ttgctttaca ttaatcactg caaaatagta cggagagtta ttatatagaa
     9601 tcgtatttcc cccctcatgg atgatcttga tatttttctc cgcatcttcc ctaccctctt
     9661 ttagtgactc aggccgataa atcatcttaa cctgagtatg caatgctatg gcaagggagt
     9721 ttgtgccctc ttttggtgct ttagggattt cctggacgtt gacccaaaac agtgattctt
     9781 tgtctttggg taaactgtca ttaattttca ggagacgcaa aatttgttta tgtccaccag
     9841 aaactttgaa aaaggtcgga ctgggggcaa agtaaacgtt ctgatctctt gcatttgcat
     9901 tatcgatcca gatttgaccg ccatactctt gagaagattc attattgacc tggactgaaa
     9961 tgttatcctt atcgcccgca aaaatataac gcgtactatt gagaacaaag gcagcatggc
    10021 tattaaagca gatcagcagc aaagccaata ctggcatgag atttattttt ttcattacat
    10081 tgttactcac agtgaatctc ctgagtttgg tcatagtttt tcttcagtga ctgcgtatta
    10141 aattgacact ggtcaagcga aataacacct tcatcgtgtg tttcaaggtt catcagcaaa
    10201 ataccaccgt tatttacata acccacttct ttaccctgtg tattttttgc gatgctaccc
    10261 ataggaacga cataaccatt tttcccataa agtttaacca cataggtatt aattttctta
    10321 aacttcacat ttttataaat gatagcattt cccgttgggg tcacattaat cgcattatct
    10381 gtaagctcaa tattcttcgg taatttttct gcatttacag aaatcgtatt attgttataa
    10441 gcagtaagtg ggattaccgc ccgaccatat ttatctcccg gtaaagaacc gttaaacata
    10501 acaccgggga catctttcat ttgcacgata gccagttgat catttttaac tggagtaaat
    10561 attatgccac cttctggcac accaattaca ctaccaccca actggagtga agtagatgaa
    10621 tttttcccag tttgtgaata cgttgctgac gtctgcatcc agtcgaataa ataggaggca
    10681 gaaagtgtac caccgatagt atcacgcgtc tggttaactg atgcgttata actcagcctt
    10741 tgattaatca ttcctgagat cccagtattc atactggtcc ccgtacgtcg atcatagtta
    10801 acattcgaaa aactataatg tgttttatca aacagactta acggcacact taatgacaaa
    10861 ccaacattat agtcagcatc catattttgc agtctggaat aaccaccgtt cagtgtcatt
    10921 gttatttgat caaaactttt tgtcagactt aagttagcac cgacctcgtt ataattatcc
    10981 cagtaatctt ccttccacgc agaaaagttt aaaaacatac ctttttctgg tagctggtag
    11041 gtaacgattg actcatagcg ttgctttggc ctatttctta taaaactata tgaattataa
    11101 gtaaaatcag catagtcaat ataatcctct gaattaaaac ggtaaccaat aagctgtaaa
    11161 tttgcattat caccaagcgc ttttgcatat tttaatgacg tgctatagcc ttttagattc
    11221 ttaccattat ggtattttgc ttgtgataag ttgccactaa aagacatggc tccaaaccat
    11281 ccaaacgagc gaattagtcc taaaccaata ttgttataat ttttatccag caataaagag
    11341 ctattgagtg taaaaggctc gaatccatag tcataactca tttgggcaaa cagactttct
    11401 ttatcccgtt cgccattatc ttggttaact ttgctaccca tggcgagggc gtagttatac
    11461 gttcccggtc ttaacaaatt aggcaacact gcgacaggga aacgctgttc acgaacggag
    11521 ccatcctctt catagatttt catgatcaaa tcgccagagt aaacgccgtt aagatcgttg
    11581 atcacaaatg gtcccggagg cacgacaata gattttaagg tataaccact ctgctcaatg
    11641 gttaccctgg catgggttcg cgcaatacca ttaattaccg gcgaataggc acgagagttc
    11701 cacggataca tatcctgatt cgatttaagc tgagcaccgt aaaaggtaaa accattattc
    11761 aatgagttat aaagctgtgt cttgcccagt tgcaaatcgg atttcagtga tttgattgcc
    11821 cgcgtcagta acaggttatt agtatcgaaa ttttcgccct ggtaagcata ccccctaccc
    11881 aataacaccc attcgcccag attcacctgg ccatcaatac tactgtaggt ggttctttcc
    11941 tggttgctat tttttgcagt attaacgtca taagctattt taaaaccgtt actaccatag
    12001 tcccacacac caccatcttt tgctgcgtcc tggaagcctg cctgtggcaa atcaatagat
    12061 acttcttgca acgattgatc aaacgcgact ttactgttcg cctcattacc cagcaaatag
    12121 cactggcgtg cagaattata aacattttta taaaaatctt tattaattat aatgccgttt
    12181 tcagttagcc attcatcaga aagacatatc ccctcagcat cctcttttgc gatgctcatc
    12241 tcagtagatg acgcgatttt agaaccattg aaaacaacat caacaacata tttacccggt
    12301 acgttttctt tattgctgtt taaaacatca ggaatatcct tactccctcc acgaataaag
    12361 ctgtagttga actcttcggc aagagcacct ttagatagta aatgaaaaat aatagcggca
    12421 ataaatattt cttttttcat tattttccct tgccataaac atcaggtaat aagaagggag
    12481 gagattaatc ctcccttcct tcgctcatat attatttata agcaacaaca taacttgctg
    12541 aagttgaaaa tgcgcctgga gtcacagtac cagtaggttt aactaacgaa gcactgtaat
    12601 caaaagatcg aataccgcca ctagttactt gatagttaaa agtagtttga ccggatttaa
    12661 catatgagct cgcaccagtt tcttgggcat tagaagtaag taatgccata tatgcatttg
    12721 ttccccccgt agttgcattg ctaaaacctt gggttgttaa attatttgat gtccagctaa
    12781 tctgggctcc attttttgcc aagcaagcat tatctgaagg aacaagctta aattgaactg
    12841 aagcaggtgt tcccgtcgtt gatacagtac ctaaatcaac tgtaggtatc tcagctccag
    12901 aagcatttgt aactttaaga tcacacgttg cgctggatac agcccctgta aaattaacag
    12961 taccactgtt tgcagccatt gcagtagaag tcatcccaag tacagcaaga atagctgaac
    13021 ctaaaaccaa ttttttcatt taatgctcct taaagccttt aaagaattgt tagccccaaa
    13081 ttcggttagt cctatatttg ttcatgccca gaagttcatg aacgtcgcaa aaattacctc
    13141 gcataagata agatggtcaa caataaaacc tcattatctg gtttttatat ctctttattt
    13201 tatttagaaa acacacgcta aattaacttt catttttatt cttgatgttt ttatttttta
    13261 tgcttgttat gaatagtctt caaattaagg acgaaatagt tttatttatt gcgccattta
    13321 tttatattta aagttatcct catggaaatt gaatgttttg ataagtaaaa tttaaatttt
    13381 agagtgtatt taacaacata atcttatctg tcacgacatg cctatagtaa aagttcaatt
    13441 gttcttttga tggcaacaga ttgtctgcta ttgaagtcgg gattttttga agaaaacata
    13501 ccatttacct tgaacatatc ttaattgtga caatgaatat tcctcaacag atcaacaaca
    13561 tgaaacgacc ttatactgaa aaatgattat agatcattta tataaatgca tacgataatg
    13621 agcgtcacct gaagtactca actctactaa aagtaattat tgttatttta ctggttaatt
    13681 agttatagtt ttttatcaaa tctttatatt tttaatgtag atattaaaaa atctttatct
    13741 tatagttgtc gcataatata aaagataata ttttaggata tgtatgaatc ttgtctttat
    13801 acttgccagt gataataatt ttttcaatta cgggatgcgg ttgatatctg atattaacaa
    13861 aacatttcga tgcatagatt ttacagaaat cgatgacata gtcagagctg attttgatac
    13921 aaatgagatt tatcttattt gtgatattaa aaattattat gcatatagtc ttctgttatc
    13981 cggaaaatcg ataaagtgta ttgatactaa taatattaga atacatcata actctatcta
    14041 cattcataaa aagaaggcat cggtaagtga agcaataaac tccttaaata atattgaaat
    14101 ggagatttta taccttttct attttcatgg taagagtgta agagagattt caaaaatgac
    14161 taatctctca aaagaaaaaa tctattatcg agtcagtcgc atcaaagtta agttggggat
    14221 gaaaacaacc aggaaattac ccgcattatt aagaattttt tttaaccaaa caattgaacc
    14281 tgaggattga ctcgttaatt acgcataatt tcattacgat aatattatta ttcttatagt
    14341 gattacatcc attacgcata taattcattg tttttttaat tatttgttag attgtttaaa
    14401 attaactcac gttgaatttt cgagtttaca caacttaata actcatggaa ttatatatat
    14461 acaaagcatg aggtggcttt atgaatagtc gagttacttg tacaaagctt gacatctctg
    14521 tcaatagaaa tctagacaaa aatcatacac ttacattaaa aaaaattcgt ttttataata
    14581 gcgcaatgat ttttattaga aatgcacaat tagttattaa agcaaaagat ggagatgtta
    14641 ttaacatccc tcctttttct ttatgctata tagaaaagaa tcttgtcgtt gatgcaacat
    14701 taaaagtgct tggcagcggt ataccttatg atatctatac tattgatagc tatctattaa
    14761 atagcgtgtg taaggttatg gagccacttc ttttaggcca tcaactagtg agtcaaataa
    14821 gaaaaaaaat atttttttat atccttgagg aaacagataa agaaattttt aaacgtctga
    14881 ttaataataa cttaccgaaa cacagacaag tttataaaat agcctattta ctttccagaa
    14941 tccatgaaat tgaacctctt gtttattcac tatcagtttc aacagatatc acttttacag
    15001 aaaaaatcaa aaaaattatc gaatcagatt tatcaagaaa ttggaagtta atggatctta
    15061 gggatatttt gcatatgtct gaggtttcga ttagaaaaaa acttgaaaaa gaaaacaata
    15121 actttaatgc gttggtgtta gatatcagaa tgcatcatgc agcaaagatg attacatcca
    15181 ctgaaaaaca tattaatagt attgcatatg atgttggata taccagtaca tcttatttca
    15241 ttcgaaactt taagagctat tttggtatta caccgaaaca gttttctctc aaggtaaaaa
    15301 gaaataatta aatgtccact tttgggcatc aaaggagcgt ttatattctc agtggactag
    15361 acaaaaaggg ctcgagtttc gctgcctgaa cagcgaattc tcatgtcctg tatttgcaat
    15421 tttttgctct caaccacatt tacaagattc aatacattaa tattatttaa tgatccaaat
    15481 aaagataatg catccaactg gtcattctta gcagcacttt ccgcatcacc gaaacatggc
    15541 tgaaatggtc ggagtagact gccacctggg cgtagatgcc gtcgggtaaa gcatcgatat
    15601 catcgttagg gtccagttca atggttccca gcacaccgtc cgtgccgggc acgacggtta
    15661 atgattgcag taccccttgc gcctgatacg aaccgcctgg cacgacaggt aaaatactag
    15721 tcagtttgcc gtggaacacc tgcccaggta gcgcgttaaa caccacttcc gcatcatcgc
    15781 cgggtttcag acgtaacagc gagttctgcc gaaattgggc gacaatttgc cgtttttgct
    15841 cggggatgaa aaccatcacc ggacgtagcg gcaaggcagc tgcgtatgta cctgggcgga
    15901 tcagtacctg agtaacgtag ccattgctcg gtgcgcgaat gacagtctgc tcaaggttat
    15961 attttgcttc agtaagttgc gctcttaagc tcacaatctg cgactgctcg ccgttaacca
    16021 tactgtcgag ctggctctgg atctgcgcct gctccgccac cgagcctttc accagcgcgt
    16081 cctgcgcgag gaaattttgc cgcgcatcgt cgatgtcacg ttccgagaac ggattcaccg
    16141 ccgcctggct gcctttcagg taacgttgat aatttttaaa cagacggtcg cgctccgctg
    16201 aaacctgggt ggtgttggcc tgcgcttcag tgagctgcgc gcgcagcgtc tttatattat
    16261 gcgtcgccgt catcaggtca gcctgaagtc tgtcaactcg cgcctggtaa cgaaccgggt
    16321 cgagcttaaa aagcacctcg cccttctgaa taagctgatt atttttgtcg gtgacttcag
    16381 taacaattcc cgtcacctgt ggcgtgatgg ggatcgctat cactgccttt tgcgcggtaa
    16441 aagtgtaagg gtggttgtag ttcatcaaca aaatcaaacc actcaccaga aacacgcctc
    16501 ccagcgccgc cgtcgccagc gtccactgat ttaccggaat gcggaagatt ttaaagaccg
    16561 cccacgccag cgccacgtaa gttaaaacaa tcaatagatc catatttaga tctccgggaa
    16621 cgtggaatag tcagtctttt tctcggcggc gagttgatgc tccagccggg aaattcggtc
    16681 agaaagtgcg gcgatttccg ggtcagtcgc tttctcctgc gacgcaacat gtgactgcat
    16741 accccagcca cgctccggtt gatagagcgt cgcccagatc cacaggaacg gccagataac
    16801 atgcagagta aacaggctca cccagcccgc tgtatgaata gcgtcggcat gggggtgatt
    16861 acgctttttg gcaatcagat aagggatgtc atgtatcgcg atgattccat aaaaaatcac
    16921 cagaaagacg aagatcagca ctcccaacgc gaagtagttt agaaacatat ctgcctcgga
    16981 ttcacgttta tcagtgttgt ttttggcggg cagccagatg ggagtcgggc tgatattttg
    17041 acaataatcc gggttggcga ttctcgccat aacaccgaag aataattttt agaggtgatg
    17101 agttgcttag ttacataacg attttatgac gaaggcataa catgctgcag atcacatcag
    17161 gtgaacgccg taagaaaata tcttgtgatt cagatcacaa agattcaaca aactaacaaa
    17221 acaaaaatgt gacattactc acatttaaat gccattttta gcgaaaatcg ccgccttgtt
    17281 gcttttttac acaagagttt tgtgatgcac atcacgtcaa ttacctctct accccctata
    17341 tttatgtgat tcatatcaca caaaaagccg ttgactggac agttaaccga ttcagtgcca
    17401 gatttcgcag tatctacaag gtccggctac ctctgccgcc acattaacaa aaaacctcgg
    17461 gcttccagcc tgcgcgacag caaacataag aaggggtgtt tttatgtcat ccgatattaa
    17521 gatcaaagtg caaagctttg gtcgtttcct cagcaacatg gtgatgccaa atatcggcgc
    17581 gtttatcgcg tggggtatca tcaccgcgtt atttattcca acagggtggt taccgaacga
    17641 aacgctggcg aagctggtcg ggccgatgat cacttatctc ctgccactgc tgatcggtta
    17701 taccggtggt aagctggtag gcggcgaacg tggcggcgta gtcggtgcca ttaccaccat
    17761 gggcgttatc gtcggcgcag acatgccgat gttcctcggt tctatgattg caggtccgct
    17821 gggcggctgg tgcattaagc acttcgaccg ctgggtagac ggtaagatca aatccggttt
    17881 tgaaatgctg gtgaataact tctccgctgg tatcatcggg atgatcctcg ctattctggc
    17941 atttctcggc attggcccgg ttgttgaagc cctgtctaaa atgctggcgg cgggcgttaa
    18001 cttcatggtt gtccatgaca tgctgccgct ggcgtctatc tttgttgaac cggcgaaaat
    18061 cctgttcctc aacaacgcca ttaaccacgg tatcttctcg ccgctgggta ttcagcagtc
    18121 ccatgaactg ggtaaatcca tcttcttcct gattgaagct aacccaggtc cgggtatggg
    18181 cgtgctgctg gcgtacatgt tctttggtcg tggtagcgct aaacagtctg cgggcggtgc
    18241 ggcaatcatc cacttcctgg ggggtatcca cgaaatctac ttcccgtatg tgctgatgaa
    18301 tccgcgtctg attctggccg ttatcctcgg cggtatgact ggcgtattca cgctgactat
    18361 cctgggcggt gggctggttt ctccggcatc tccgggttct atccttgctg tactggcgat
    18421 gacgccaaaa ggtgcttact tcgctaacat cgcgggtgtg tgtgcggcaa tggcagtctc
    18481 cttcgttgtc tctgccattc tgctgaaaac cagcaaagtg aaagaagaag atgatattga
    18541 agcagcaact cgtcgtatgc aggacatgaa agctgagtct aaaggtgcgt ctccgctgtc
    18601 tgcgggcgat gtgactaacg acctgagcca cgtacgtaaa atcatcgttg cctgtgacgc
    18661 aggtatgggt tccagtgcga tgggcgcagg tgtgctgcgt aagaaaattc aggatgcagg
    18721 tctgtcgcag atttcggtca ctaacagcgc gatcaacaac ctgccgccag atgtggacct
    18781 cgtcatcact caccgtgacc tgaccgaacg cgctatgcgc caggttccac aggcacagca
    18841 tatttcgctg accaacttcc tcgacagcgg cctgtacacc agcctgaccg aacgtctggt
    18901 tgctgcccag cgccatactg aaaacgaagt aaaagtgaaa gacagtctga aagacagctt
    18961 tgacgattcc agtgctaacc tgttcaagct aggcgcggag aacatcttcc tcggtcgcaa
    19021 agcggccacc aaagaagaag cgattcgttt tgctggcgag cagctggtga aaggcggtta
    19081 cgttgagccg gaatacgttc aggcgatgct ggatcgtgaa aaactgaccc cgacttatct
    19141 gggtgagtct atcgcggtgc cacacggtac ggttgaagca aaagatcgcg tactgaaaac
    19201 gggcgtggtg ttctgccagt acccggaagg cgtgcgcttc ggtgaagaag aagatgacat
    19261 tgcccgtctg gtgattggta ttgctgcccg taacaacgag cacattcagg ttatcaccag
    19321 cctgaccaat gcactggatg atgagactgt catcgagcgt ctggcacaca ccaccagcgt
    19381 ggatgaagtg ctggaattgc tggcaggtcg taagtaatcc aatcctaccc tctcctcacg
    19441 gagagggttt gggtgaggga aaagcctcac cccagccctc tcgggtaaaa acattgatga
    19501 aggttaatac tatgaaagca ttacattttg gcgcaggtaa tatcggtcgt ggctttatcg
    19561 gtaaactgct ggcagacgcg ggtatccaac tgacgtttgc cgatgtcaat caggtggtac
    19621 ttgatgccct gaatgcccgt catagctatc aggttcatgt ggttggtgaa accgagcagg
    19681 tagataccgt ttccggcgtc aatgctgtca gcagcattgg tgatgatgtc gttgatctga
    19741 ttgctcaggt tgatttagtc actaccgccg tgggcccggt tgtgctggaa cgtattgccc
    19801 cggcaatcgc caaagggctg gtgaaacgta aagaacaagg taatgaatcc ccgctgaaca
    19861 tcatcgcttg tgaaaacatg gtccgcggca ccacacagct gaaaggacat gtgatgaacg
    19921 ccctgccgga agatgccaaa gcgtgggtag aagaacacgt tggctttgtt gattccgccg
    19981 ttgaccgcat cgtgccgcct tcggcttctg caaccaacga tccgctggaa gtgacggtag
    20041 aaacattcag cgaatggatt gttgataaaa cccagttcaa aggcgcgctg ccgaacatcc
    20101 caggcatgga gttaaccgac aacctgatgg catttgtcga acgtaaactc ttcaccctga
    20161 atacgggtca tgctataacc gcgtacctcg gaaaactggc gggtcatcag accattcgtg
    20221 atgcgattct cgacgagaaa atccgcgcgg tggtaaaagg tgcgatggaa gaaagcggtg
    20281 cggtactgat caagcgctac ggctttgacg cagacaagca tgcggcatac atccagaaaa
    20341 tcctcggtcg tttcgaaaac ccgtatctga aagatgatgt agagcgcgta ggccgtcagc
    20401 cgctgcgtaa actgagcgct ggtgaccgtc tgattaagcc actgctcggt acactggaat
    20461 atggtctgcc acataaaaac ctgattgaag gtattgccgc tgcaatgcac ttccgcagtg
    20521 aagatgatcc gcaggctcag gaactggcag cactgatcgc tgacaaaggt ccgcaggcgg
    20581 cgctggcaca gatttccggt cttgatgcca acagcgaggt tgtatccgag gcggtaaccg
    20641 cttataaagc aatgcaataa tggtggacca ggcgcaggac accctgcgcc cgaataacag
    20701 attgtcagat atgcaggcaa caatggaaca aacccaggcc tttgaaaacc gtgtgcttga
    20761 gcgtctgaat gctggcaaaa ccgtgcgaag ctttctgatc accgccgtcg agcttttgac
    20821 cgaggcggta aatcttctgg tgcttcaggt attccgcaaa gacgattacg cggtgaagta
    20881 tgctgtagaa ccgttactcg acggcgatgg tccgctgggc gatctttctg tgcgtttaaa
    20941 actcatttac gggttgggcg tcattaaccg ccaggaatac gaagatgcgg aactgctgat
    21001 ggcattgcgt gaagagctaa atcacgacgg caacgagtac gcgtttaccg acgacgaaat
    21061 ccttggaccc tttggtgaac tgcattgcgt ggcggcgtta ccaccgccgc cacagtttga
    21121 acctgcagac tccagtttgt atgcaatgca aattcagcgt taccaacagg ctgtgcgatc
    21181 aacaatggtc ctttcactga cagagctgat ttccaaaatc agcttaaaaa aagcctttca
    21241 aaagtaagta ctttcgctta cttctcctct acctgtttcg gccgataaag ccgacgataa
    21301 tattccagac gttgtagata taacggcacg ctttcatcag gtattccgga cggaatcgcg
    21361 ttacggggag gtagtttttt cagatactcc cggaacgcct ggctcgatgc catgaaatct
    21421 acggctttat cgataagaag cggaacgttt tcacctaatt tgcccatgat gatatctccg
    21481 tattaccccg cgccgggaat gcgcggccgc caattttagt ttagatcccg gtataaatga
    21541 cattaaggaa aacgtgctca atcctcaata atcatccgtt tctattaact atcttttttc
    21601 tttaaatttc ttaattaatc aataaattgc gtagttttta agcatgttaa tgtttacgtt
    21661 atagaactat tgcaatgtca tcagttgtga acaaagcacc tggtcgcgca tactaggggc
    21721 tataaattta tctttatcag aagccatcac atgaaagaag tcgaaaaaaa cgaaatcaaa
    21781 cgtctcagcg atcgtctgga cgccatccgc caccagcagg ccgatctgtc gctggttgaa
    21841 gccgcagaca aatacgccga gctggaaaaa gagaaagcca ctctggaagc agaaattgct
    21901 cgcctgcgcg aagttcacag ccagaaactc agtaaggaag cacagaagct gatgaagatg
    21961 ccgttccagc gcgcgattac taaaaaagag caagctgata tgggcaaact gaagaagagc
    22021 gttcgcggac tggtcgttgt gcacccaatg accgcgctgg gccgcgaaat gggcctgcaa
    22081 gagatgacag ggttctctaa gaccgcgttt taagttcctt tctcgtcgct gtaattacag
    22141 ccactcacga tttactcgct cctgctggca catgcaggag cctgtttcag agatcgaata
    22201 tttataacgt tcttaacatt atatattcaa tctcctctat tctttttagc gcacatgcca
    22261 ggctaaattc tgaaaagcag gtacaaatta aataatgatg tatcaatatc aacaatgaat
    22321 taccgcatat caatgcttga aacataatta attatttaaa tttttctatc agcatcgcaa
    22381 acttcgcaat aaaacatcac tcaactgaat atttatcttt ccctgccccg ggcctttatc
    22441 cggtgaaaca tttgacattg attgacaccg atcattaaaa tccgcgcgat taatattatt
    22501 ggctacaaac tgaaatcgtt tgacatttaa taacacattc gcacaaccaa ttaatataaa
    22561 attctctaac aaatttattt tgcgaatatg ccccactcca atcattcata aaaaaaatga
    22621 ttgtgccgta attatcaact gtatcgggat tatgaaaagg aattcatcct atgaacctaa
    22681 ataaaacatt gttaagcgtg ttaatgatat tgccactttg cttattggca gggtgtgact
    22741 atattgaaaa agcgactaag gtcgacgacc tcgttacaca gcaagagtta caaaaaagca
    22801 aaattgaggc gcttgagaaa caacaggaac tcgataagcg caagatagaa tactttgaaa
    22861 aacaacaaac tactatcata aacagtacca aaacgctcgc tggtgtggtg aaggcagtta
    22921 aagacaagca ggacgaattt gtctttacag aatttaaccc ggcacaaacc caatacttca
    22981 ttttaaataa cggctctgtt ggtttggcag gccgggtgct ggctattgat gcagttgaaa
    23041 atggcagcat tattcgtatt tcgctggtta atttattaag cgttcccgta tcaaatattg
    23101 gctttcatgc gacatggggc aatgaaagac ccaccgatgc caaagctctg gcaaagtggc
    23161 aacaattact tttcaacacc acactgaatt ccactctgca attaatgcct ggtcaatggc
    23221 aagacattaa tctgacatta aaaggcgttt cgcctaataa cctgaagtat ctgaagttgt
    23281 ccatcaatat ggccaatctc cagttcgaca ctgttcaatc tgctgaaact cggcagcgaa
    23341 aaaacaaaaa ataaatgcag cttctgcgct tatatcaagg aatagagagc atcaataatg
    23401 aacaaaattt ttaaagttat ctggaatcca gcgacgggca gttacagcgt tgccagcgaa
    23461 acggcgaaaa gtcgtgggaa gaagagcggg cgcagtaagc tgttaatttc tgcactggtt
    23521 gcgggtggat tattgtcgtc gtctggggca ttagctcagg cagggttaga tacaggtact
    23581 ggtgttaccc ctgctggtca taataatgga acaggttgga tagctattgg taccgatgct
    23641 gaagcaagca ctcataccac aacgaatggc gcagcaactg ccgtgggcta ctactccaaa
    23701 gcgttaggta tgtggagtac tgcgttaggt gcatatagcg aatcgaatgg caatgcttca
    23761 ctggctcttg gggttaaagc acaatctaac ggtgaccgct ctatttcgat gggcgcttcg
    23821 tcgagtgcaa gcataaatgc gggttactcc atcgcgatgg gggtatttgc tttcaccgat
    23881 gcagagtacg cggtagccct tggcaatgag agcaaggcac ttggtaaata cagccttgca
    23941 ttaggaaacg caagtcaggc atctggcgaa tccagtattg cattaggtaa cacaagtgaa
    24001 gccagcgaac aaaacgcgat tgcgctgggg caaggcagca ttgcgagcaa agtgaactca
    24061 atcgcgttgg gcagtaacag ttcgtctgca ggagagaatg ccatcgcgct aggagagggc
    24121 agtgccgcgg gaggcagcaa cagtctagct ttcggtagtc aatccagggc aagcggcaat
    24181 gattctgtcg ccctcggtgt aggggctaca gcagcgaccg acaattctgt cgctatcggc
    24241 gcaggatcga ccactgatgc aagcaataca gtttcagttg gcaacagcac aacaaaacgc
    24301 aaaattgtta atatggctgc cggtgccata agcaacacca gtaccgatgc catcaacggc
    24361 tcacagcttt atacgatcag tgattcagtc gccaaacggc tcgggggagg cgctactgta
    24421 ggcagcgacg gcaccgtaac cgcactaagc tacgcgttga aaagcggcac ctataataac
    24481 gtgggtgatg ctctgtcagg aatcgacaat aatactctgc aatggaatag aaccgcgggg
    24541 gcattcaccg ctgcacacgg ctcaaatacc accagtaaaa tcactaatgt tgctaaaggt
    24601 acggtttctg caaccagcac cgatgtagta aacggctctc aattgtacga cctgcagcag
    24661 gatgctctgt tgtggaacgg caccgcgttc agcgctgcac acggcaccga cgtcaccagc
    24721 aaaatcacca atgtcaccgc tggtgagctc tctgacacca gcaccgacgc cgtcaacggt
    24781 tctcagctga aagcgaccaa ggacgatgtg gcggcaaaca ccaccaacat cactaacctg
    24841 acgagcgaag tggctggcaa caccaccagt atcactaacc tgactgatac ggtgactaac
    24901 ctcggtgaag acgccctgaa atgggacgac gccgcaggcg cattcaccgc tgcacacggc
    24961 actaacgcca ccaacaaaat caccaatgtc accgctggcg aactctctga taccagcacc
    25021 gacgccgtca acggttctca gctgaaaacc accaacgata acgtggcgac caacaccacc
    25081 aatatcgcca ctaacaccac caatatcacc aacctgacta acgctgttga cagtctcggt
    25141 gatgattccc tgctgtggaa caaagcggct ggcgcattca gcgccgcgca cggcaccgat
    25201 gccaccagca aaatcaccaa cgtcaccgct ggcgacctga ctgctggcag caccgacgcc
    25261 gtcaacggtt ctcagctgaa aaccaccaac gataacgtgg cgacca
//
LOCUS       DAMZFT010000072        25173 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.072, whole genome shotgun sequence.
ACCESSION   DAMZFT010000072 DAMZFT010000000
VERSION     DAMZFT010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 25173)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25173)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25173
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.072"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            104..1015
                     /locus_tag="PN977_002956"
     CDS             104..1015
                     /locus_tag="PN977_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001730253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HDI6042973.1"
                     /translation="MRFARLASKSASRGIRDMAELTKEWLKQTIAEYEANRDELPFGL
                     DTNSAIGLQAFKLALASLEADPVAYIFKHPAGKLFWALTDESNKEQADVIPVYAAAPA
                     SVVPDNASEPLAYAYKELTPEIMRNHLAVFERYGIAPNDSSTTIQALRIALDGIERSA
                     AMLHSAEPASNHEELPLDYLQGQKDGLEWAAQLAEANHPQTGDWLYDDPLDLAKAIRK
                     GPDIPEFAGPHPVTPDGWISCSDLMPEDTKMLLAFSQGEIVAAYWNWVVNPIDYKKYR
                     AFTYLSGNILDDVTHWMPLPEPPQEVK"
     gene            1015..1365
                     /locus_tag="PN977_002957"
     CDS             1015..1365
                     /locus_tag="PN977_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000386645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2591 family protein"
                     /protein_id="HDI6042974.1"
                     /translation="MDYSQLSDFEINRMVGDIIFKGLWACKPETSGNNTNKWYYGNTD
                     TTFEPLNPLPDYCNNPSDAWPIIVDNKISLTCHQSRGEWSAVFNVENICFHANNPLRA
                     AMIVFLMMQENQNG"
     gene            1358..1696
                     /locus_tag="PN977_002958"
     CDS             1358..1696
                     /locus_tag="PN977_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001617187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6042975.1"
                     /translation="MAKSAAERKAAQRARQAASGVRKLEIVLDAQEIEMLERNCAARR
                     PGRAPYEFGEYIALLIRQDDARVRGRIKSISRKRCGKCGERVPVNSCPCNGDSQCWVT
                     KGWYETKLIV"
     gene            1737..2411
                     /locus_tag="PN977_002959"
     CDS             1737..2411
                     /locus_tag="PN977_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001090114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HDI6042976.1"
                     /translation="MNTAANYGGFILHGTITTTVTITTVVMMPAEPKTYKRKSTQFKP
                     LTAMQEAYCQSYIKTPENQTQAAINAGFSPNTAAVKASVMMRDERIQKRIAELMEERN
                     KRMRVSADYVLMRLVEIDQMDVIDILNDDGSLKPIREWPKIWRTTLSGFDLSSTIMNM
                     NEDSIETILKKIKWPDKVKNLELIGKHVDVNAFKERLDVNVNVTIADRIAAARKRLKE
                     RQDGNQ"
     gene            2408..3883
                     /locus_tag="PN977_002960"
     CDS             2408..3883
                     /locus_tag="PN977_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016236153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HDI6042977.1"
                     /translation="MTDTALSPEEQLIEDIAGFTHDPLGYALYAFPWGEEGTELAHAT
                     GPRQWQADAFREIRDHLQNPETRYQPLMLARASGHGIGKSAFISMLINWGMSTCEDCK
                     VVVTANTDNQLRTKTWPEIIKWSNLAITKDWFTCTATAMYSNDPGHDKRWRADAIPWS
                     EHNTEAFAGLHNERKRIIVVFDEASNIADLVWEVAEGALTDEDTEIIWVAFGNPTRNT
                     GRFRECFRKYKHRWKTAQIDSRTVEGTNKQQLQKWVDDYGEDSDFVKIRVRGIFPDAS
                     ELQFIPTGLTDEAMKRVVTAAQVAHAPVIIGVDPAYSGVDDAVIYLRQGLHSKVLWTG
                     NKTTDDLIMAKRIADFEDQYQADAVFIDFGYGTGLKSIGDGWGRTWQLVPFGGASTDP
                     QMLNKRGEMFNSCKTWLRLGGMLDDQETADDLSAAEYKVRVDGKIVIEPKEDIKERLG
                     RSPGKGDALLLTFAFPVSKRLRIPGQQNQQGKAITDYDPYA"
     gene            complement(3974..4396)
                     /locus_tag="PN977_002961"
     CDS             complement(3974..4396)
                     /locus_tag="PN977_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000848285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6042978.1"
                     /translation="MKTIDMLAKYLNEWPLKYSRIVQAEDCVFYGVFAGNEMHYEVIQ
                     SEGLTGLTLSEDHGTSVTFHDWITAQRSEMEKGDVFDISRSVCAKAKSDNDYMSEHLY
                     NMKLQCLAEVLGKKAIVDKQGAISEAKAINAAFDEIIF"
     gene            complement(4595..4671)
                     /locus_tag="PN977_002962"
     tRNA            complement(4595..4671)
                     /locus_tag="PN977_002962"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4635..4637),aa:Arg,seq:tct)
     gene            5099..5305
                     /locus_tag="PN977_002963"
     CDS             5099..5305
                     /locus_tag="PN977_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6042979.1"
                     /translation="MCMGSSPSVPATPEVQAAPQEQDAAVVDARDEETRRRRAAAGRS
                     STLLTGSQGDTSTANTSGKTLLGQ"
     gene            5320..6999
                     /locus_tag="PN977_002964"
     CDS             5320..6999
                     /locus_tag="PN977_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDI6042980.1"
                     /translation="MAETTKERLNKQFAQLESERQSFEPHWRELSDYINPRGSRFLTS
                     EVNRNDRRNTRIIDSTGTMAARTLASGMMSGITSPARPWFRLATPDPEMMDYGPVKLW
                     LEAVQNRMNDMFNKSNLYQSLPQLYGSLGTYSTGAMAVLEDDEDIIRTMPFPIGSYYL
                     ANSPRGSVDTCFRKFSMTVRQLVQEFGLNNVSESVKSMWESGTYEKWIEVMHSVYPNI
                     DRDTSKLDSKNKPFKSVYYEVGGDNDKLLRESGFDEFPIMAPRWEVNGEDVYGSSCPG
                     MLALGPVKALQLLQKRKSQLIDKATNPPMVAPTSLKNQRASLLPGDITYIDQITGQDG
                     FRPAYLVNPSTADLVADIQDTRQIINSAYFVDLFMMLQNINTRSMPVEAVIEMKEEKL
                     LMLGPVLERLNDECLNPLIDRAFSMMVRKNMLPPPPDAMEGMPLKVEYISVMAQAQKS
                     IGLSSLASTVNFIGQLAQAKPEALDKLNVDQAIDAFADMSGVSPTVIVPQEQVEQARQ
                     QRAQQQQQQQMMAMGMAAAQGAKTLSEAKTSDPSVLSAMANAVSGQGGQSQ"
     gene            6996..7292
                     /locus_tag="PN977_002965"
     CDS             6996..7292
                     /locus_tag="PN977_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001683132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6042981.1"
                     /translation="MTDYEDDQLKEENARKQRDMAQREIDDIRFVMSSEQGRRVVWSV
                     LEKGRVFSAISPMDAMAMAFNEGQRNLALELFQRVMAHCPEQYLKMAKEASEQE"
     gene            7295..7990
                     /locus_tag="PN977_002966"
     CDS             7295..7990
                     /locus_tag="PN977_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001048067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HDI6042982.1"
                     /translation="MNLFERLLYRRLCNEQPVDGGAAPAASEPSAPAGDNHAPVGDPS
                     QPEGDKSQPVADGDKPADDKNPENDKQDEKKDGDKPEGAPEKYEFQAAEGVELDTEAL
                     KEFEPVARELNLTNEQAQKLVDAYPKILAGVQQRQVEAWQKTTEQWAADVKADKEIGG
                     DKLISNLSAAQRALDQFGTPELKEYLNTTGLGNHPDLVKTFVKIGKAMSEDGMVTGGN
                     EGQRSAAEVLYGK"
     gene            8005..8991
                     /locus_tag="PN977_002967"
     CDS             8005..8991
                     /locus_tag="PN977_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000268714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6042983.1"
                     /translation="MAVKGLTALTLADWGKRVDPNGKVDKIIELLGQTNPILQDMPFV
                     EGNLPTGHRTTIRSGLPSATWRLLNYGVQPSKSTTVQVTDSVGMLETYAEVDKSLADL
                     NGNTAEFRLSEDRAFIEAMNQQMAQTLFYGDSSVNPQQFMGLSSRYSSLSAGNAQNII
                     DAGGTGTDNTSIWLVVWGENTVHGIFPKGQKAGIQMEDKGQVTLEDANGGKYEGYRTH
                     YKWDNGLALRDWRYVVRIANIDVSNLSEPSSAANIAKLMVKALHRIPNRGMGRPVFYM
                     NRTVGQALDLQSLEKTSLAISVKETEGEWWTSFRGVPIRETDALLETEARVV"
     gene            9043..9480
                     /locus_tag="PN977_002968"
     CDS             9043..9480
                     /locus_tag="PN977_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6042984.1"
                     /translation="MITDKLLMFSEAQAVTNTAASTDVIDLGPIDGKRRDIGVGYPLE
                     FWVLVNAAAAASGDATVNIQLQTSENNTSWTTIYDSGALVKTALTAGKRVVSAKVPAG
                     VQRYLRVNYSVATGPLTAGEFTAGISLDVDANTPYPIRSKVTG"
     gene            9491..9826
                     /locus_tag="PN977_002969"
     CDS             9491..9826
                     /locus_tag="PN977_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000012377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6042985.1"
                     /translation="MSGEKPRYRVLRLSHIHNTLWPEGAEIEYEGEPGSALEPVNDAA
                     RQAKAKVAGKVSMAATSTKIINDVSDDGELDKLREEYELLFNEKPHHNAKAETLREKI
                     ADKRKELGV"
     gene            9877..10200
                     /locus_tag="PN977_002970"
     CDS             9877..10200
                     /locus_tag="PN977_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016236149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6042986.1"
                     /translation="MELVNLKTGTDSYQDESGETRTRDEYPWGLCITLNNDTLNKLKA
                     QPKGVGTEVMITAKAVIRGLSARETDDGVNRSADLQITDMAIAPVSGDVEKSAAETLY
                     GNGGE"
     gene            10200..10805
                     /locus_tag="PN977_002971"
     CDS             10200..10805
                     /locus_tag="PN977_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001544819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6042987.1"
                     /translation="MASVVEICNRALSNIGNSRSINSLTEASKEAGECSLHFEACRDA
                     VLSDFDWNFATKRVALADTSNPPPDWEYAYQYPSDCLRITEIMLPGVRNPTAAMRVQY
                     EVGADTNGTGKLIYTDQPQAWLKYVSRVTDVNMFDAIFMEALAWRLAAAINMALTGNA
                     DLGTFALNMYNRVILSAGSHSQNESQEPQPPVDEFTIARLS"
     gene            10805..13276
                     /locus_tag="PN977_002972"
     CDS             10805..13276
                     /locus_tag="PN977_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001018555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6042988.1"
                     /translation="MAISWIQPSFAGGEIGPSLYGRIDMAKYQVALRKCDNFIVRQYG
                     GVENRPGTRFVGAAKYPNRKCRLIPFQFSTVQTYALEFGHQYMRVIKDGALVLNSSNV
                     IYEIATPYTEADLFRIKFTQSADVLTLVHPAYPPKELRRYAHDNWQLVDVVTKNGPFE
                     DINIDESVTVYASASTGTITLTASASIFGAEQVGKLFYLEQPAVDSVPVWETSKSTSI
                     GDIRRADSNYYRAVTAGKTGTLRPSHTEGTSWDGWGGSGDDDTGIEWEYLHSGFGIAR
                     ITAVNGTTATAEVISYIPSQVVGEDNASYKWAKYAWNSVNGYPGTVVYYQQRLYFAAS
                     TAFPQTIWASRTGDYKDFGKSNPTQDDDRIIYTYAGRQVNEIRHLIDVGSLVALTSGG
                     EYVITGDQNKVLTPSSFAFSSQGSNGSSNVPPIAVANIALFVQEKGSVVRDLAYSFDV
                     DGYQGNDLTILANHLFQKHSIVDWCFSIVPYSSAFCIRDDGKLLVMTYLRDQQVFAWA
                     PQSSTGKYESTCSISEGNEDAVYFVVNRTVNGQTVRYIERLSSRLFTSDEDAFFVDSG
                     LSYDGRNTSDRTMTITGGSGEWDYRAEYTISISGGAYFTSSDVGAQLQFPYTGTDPDT
                     GDEVSKELRCDIISVTSNTAVVVRPNRNVPSSLRNVATTNWQMARRTFGGLSHLEGQI
                     VNILSDANVEPQKVVSGGAVTLESPGAVVHIGLPITAEFETLDINISGQETLLDKKQV
                     IPSVTLVVNASRGIWATTPGGKWYEYPQREFEFYDDPVDDATGKVEVKLDSNWGKNGR
                     VKIRQLDPLPLSVLAVIPRLTVGGF"
     gene            13276..13740
                     /locus_tag="PN977_002973"
     CDS             13276..13740
                     /locus_tag="PN977_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020231255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6042989.1"
                     /translation="MIDVQIIPATEEHLQMILPDVRQADIDELYAVSLMTTEDALRVG
                     LRTATMAWSGFANGELVTMFGVSPASMIGGNGTPWLVGTSRIEKYQKTFLRHCRPVLQ
                     QMLAVYPRLENYVDERNHVAKAWLHWLGFRLEEAAPYGALGLNFHRFHMERK"
     gene            13740..14285
                     /locus_tag="PN977_002974"
     CDS             13740..14285
                     /locus_tag="PN977_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000332883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6042990.1"
                     /translation="MCDPVIAGGAMLAMSGIQAYTQYQQGKYASKVAEANADIATAQA
                     NDAINRGNAEAEQRRRETRQRLGTQAATMGATGADLSTGNALDIFGDTAQFGALDSLT
                     TVNNAQREAYGYQVQAANYKAEASSARKQGNVGAATTLLTAPLKAYGAYQMFGGTWSP
                     FTQSTPAPIGAAAGTRLPGGL"
     gene            14285..16798
                     /locus_tag="PN977_002975"
     CDS             14285..16798
                     /locus_tag="PN977_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6042991.1"
                     /translation="MPVVPTVSGRQVESRGVQSAGLQTFSQPGIGDAFVRAGAEAIDV
                     FGQAKQRANIALAQEASLNLSQISSDLLNNPETGLLNLKGKNAIGKGHEYTQQFDAQV
                     EQLAMSLPDEQARNAFMQQAQQQRIQFTTQAGRHEIGQINAYEEGQFQATLLNNGKNA
                     AALYGDNAAYVLANKQTFQQIEDYGIAHGWSDEQIQAKKIEFKEATAKATAQNAIGAN
                     YLQVRQQNGELSDTAAGSRRAVADSGSFDRTRGIRNNNPGNLEYSKTNPWVGQTGDDG
                     RFAKFETPEHGIRALGRNLMSYQRQGIDTVSEIINRWAPPTDKNDTMSYIKAVCEQLG
                     VSADESLDASNPDTLKALCAAIIHHENGSQPYSDQQLTDGVSAALGLSQLPTKNKRYT
                     GVAWFDALSESDQASVLRQTDALARQQQAEYKTMLDSRVRDATAAYMRGVEFPNPPGE
                     DDFIAAYGVREGNLRYTEFKNTQIAGQYIGSFRNMPTSSITAYVEQLRPDTGDTGEGY
                     AARAALYDNVVSAANQVIKQRQSDPVQFSLASGQAKPIDMSNKDNFGQSVALRAAQVS
                     DLAKSYGTPLTFFSKDEANQIGVFFRDAPVSQQAAYLDTIRQSTGGGQVYMSALQQIS
                     ANAPSAAVAGILMDKPGGILAEKNWFNPDVSVSPETAAQTILAGAAARKGTDDAKGIP
                     MPKDADLRLEFSDMVKDAFAGDAQGASMAYEIAKDYYAGVMAKKGVVSGEIDNDVWKQ
                     AVNVATGGVHDYNGMGNVLLPWGMSAEQFDKQVNQAWNEQVVGSGIKTPPGQYGLQSY
                     GDSQYLVKLGTGYLLKDDGSPVVLNLTQKRQRFSGDIPQ"
     gene            16795..18597
                     /locus_tag="PN977_002976"
     CDS             16795..18597
                     /locus_tag="PN977_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000119843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6042992.1"
                     /translation="MSYFGLNPVNQNQQLDEAASNPAGFNSDVGFFDNAVGAALSGLY
                     SGLVAKPDQLLWAGMDKIVSPIAQFVNENTSLNDTSVSYIAEQRKLAEQQVKRLTPDA
                     ATTGTAGQVLYGLFDMGGQAVVGTTLGGPVGGAAAVTSLQGFSEFERLTAQGVDFRTA
                     QEAGLVQGITAGAGTLIPMSLGLRAGGALAEGVAAQLARTGESSVRRAAATAVRATPD
                     IAYAAGTNIAFGMAQRGLTAKTLRDGGYSEMANQYDVLDRQAIAIDAVLGVAFGGVGR
                     FINSRGESTNAPNFSPVDIDAALAANAAHHAEIDIAPGVPINVLSRNSHIQALRKAMS
                     DVSQGRPVDVASIVESASFSEIPGRKSLLSQAVNEALSSVDDGVTARAIENRLLEEQA
                     AQLLPRGDRQVYQSEIANSQRIIENLTEQRAQILAEEPTGSGKALSRARSDKQARLRD
                     IDQRIRQAQERLEFSRNALAPHEPGGQFFEARAELARRQQAESELNAQAVSFYKTAEV
                     RTPDEVAPFEPDKILQQAEQKMMSDQAGDIDLRIAEDSLLESPDMIITVLDDDGNPQS
                     RSAREVLDEANRESEQAIQDSSLFDVAVACFLRG"
     gene            18603..21077
                     /locus_tag="PN977_002977"
     CDS             18603..21077
                     /locus_tag="PN977_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001248457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6042993.1"
                     /translation="MRQECIQAVQQAAQRTLTAREIQNIEDRIYRNMRSIARDDPMSW
                     RQLSESERLYRAAQLASEELQREAALKKRRVALTIAARQRLDKFINSYQGADGKLGAL
                     NRTIAFNADGKSNFLSVESRTKATRDYALSQLQEAFEAVDPRFFGLFEDEAGVRDLIY
                     EMRGQNTGNAKARKGAKAWREVTELLRRRFNDAGGDIGYLENWGIPQHHSMEKVGAVS
                     KDKWVSDVIGKLDRKYYTRADGQLMNDAELSAFLGEAYNTIATGGLNKLTDTGMRISG
                     ARANRGNASRQIHFKDADSYLQYQQLYGDRSLWEIMVGHLEGISKDIALVETYGPNPD
                     HVFRSLLDQVKAETATANPSKTGKVERLANNTENLYNFISGKTQPVANPHIARWSDNI
                     RNWLVASRLGSALLSSFSDLGTMYLSAKVTNLPMNQLFRNQLEAMDPTNRTELARARR
                     AGLAMESLLGSVNRWAMDNMGPSVSRWAATAVMRASGLTAWSDAHKRAYGVTMMGSLG
                     EVVSRTPDLRRLDDSDFRILKSKGITDTDWSVWKLAQQEDWGNGNNTMLTPESIMRIP
                     DSAVKHLGEPERVKFEAMRKLLGAVTEEVDMAVITPGAREQLITGSGIQRGTWKGELT
                     RSVFLFKSFPISVVMRHWSRAMGMPSAGGRAAYIATFIASTTILGALSQQLNDLASGR
                     NPREMTGEDAAKFWLGALLKGGGLGLYGDFLLSDHTRYGSGALASMLGPVAGLVDDVV
                     KIAQGIPLNAVEGKSEQTGGDLVKLGKGLMPGANLWYLKAALDHMIFNQMQEYFSPGY
                     LRKMEQRSKKEFNQTYWWRPQDVTPQ"
     gene            21273..21569
                     /locus_tag="PN977_002978"
     CDS             21273..21569
                     /locus_tag="PN977_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001147904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6042994.1"
                     /translation="MQAIGFIVYIVVGLFQLAAIMAGLESWWGLHWIIAAPIAFIVSY
                     IPFVGAIVGMVGAVDVWRWEWWQAGLLFFGGIIFAIVCGGMSSFFEWLSFRKGT"
     gene            complement(21601..21762)
                     /locus_tag="PN977_002979"
     CDS             complement(21601..21762)
                     /locus_tag="PN977_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000708858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transmembrane anchored protein"
                     /protein_id="HDI6042995.1"
                     /translation="MKKAFAALFVLLSLVASTQAFAGRCQHDSDTAADGSRCGGRSAD
                     SRPGGGGIR"
     gene            complement(21855..22295)
                     /locus_tag="PN977_002980"
     CDS             complement(21855..22295)
                     /locus_tag="PN977_002980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000671199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6042996.1"
                     /translation="MKFNIRISNSFLHGESNTPFAVDGPFLTDDEIKIIQSFLEDVAN
                     GRALVGKNKPSWVDDNHDKIPGSDNYEQENYWHYHCGPTWYPNTFKNYTINLNFNPSG
                     RHSNECIHYAKNDNEIVIVGFSREHIPFLSSDGNNNPLFNDEEE"
     gene            complement(22316..22483)
                     /locus_tag="PN977_002981"
     CDS             complement(22316..22483)
                     /locus_tag="PN977_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6042997.1"
                     /translation="MTALKNRTQHNEQAKQCWDVIGKVMLGRASKERDKDMVYQTGTS
                     FSDFKAAFRSK"
     gene            complement(22577..23287)
                     /locus_tag="PN977_002982"
     CDS             complement(22577..23287)
                     /locus_tag="PN977_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001617183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BRO-like protein"
                     /protein_id="HDI6042998.1"
                     /translation="MANAQTAIFKFESVNPIRSIIIDGQPWFVAQDVCSALRIQNVTQ
                     ALEKLDDDERSMFNIGHEHRAIFDSRVKEINIISESGLYTLILRCRDAVTPGTIPYRF
                     RKWVTGEVLPQIRRTGSYIKNSLPQEERIKMVADQVANATASAVMQAMKIENKTYSAP
                     LKPGYRSLIHSPSGVLGLTENSLLMNLLNQLQEDGHDVSGAAAELTTMFCYIVGVSKC
                     LRDIQTHAEYINDKAGFF"
     gene            complement(23603..23860)
                     /locus_tag="PN977_002983"
     CDS             complement(23603..23860)
                     /locus_tag="PN977_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6042999.1"
                     /translation="MQQYHYPLEDGFTERIHTPGGVRSLVEGSHLMKLLRDLDKDGFN
                     VDGPLAELTALINYVTSSQMSMQDLQTHLDYCAEQLRKQTR"
     gene            24056..24664
                     /locus_tag="PN977_002984"
                     /pseudo
     CDS             24056..24664
                     /locus_tag="PN977_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000218909.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
BASE COUNT         6508 a         5731 c         6671 g         6263 t
ORIGIN      
        1 cgggaggttg tactgccgcg tgcgcacgat gttcacccat tagggccgca gtcggcgaaa
       61 attttttgtg agtttcaccg gagtatcgtg aacagatgcg tcgatgagat tcgcaaggtt
      121 ggcgtcaaag tcagcatcaa ggggaattag ggatatggct gaactaacca aagaatggct
      181 gaagcaaact atcgctgaat acgaagccaa tcgtgatgaa ttaccgtttg gactggatac
      241 caacagtgcc attgggcttc aggcgttcaa gttggcgctg gcatcgctgg aagcagatcc
      301 agttgcttat attttcaaac atccggccgg gaaattattc tgggctttaa cggatgaaag
      361 caataaagag caagcggacg ttattcctgt ttatgctgcc gcgcctgcgt cggttgtgcc
      421 ggataatgca tcagagcctc ttgcttatgc ttacaaagag cttacgcctg agattatgcg
      481 caaccattta gctgtattcg agcgatatgg aatagcccca aacgatagct ctaccacaat
      541 tcaggcactg cgaatcgcgc tggatggcat agagcggagc gccgccatgc ttcatagtgc
      601 cgaacctgca agtaatcatg aagagttgcc gcttgattat ctccaaggtc aaaaagatgg
      661 tcttgaatgg gctgcgcagc ttgcagaagc aaatcaccca caaactggcg actggcttta
      721 cgatgacccg ctggatctgg ctaaagctat cagaaaaggt cctgacattc ccgagttcgc
      781 tggaccccat ccggtaactc cggatggttg gataagctgt agtgatctaa tgccggaaga
      841 caccaaaatg ttactggcat ttagtcaagg tgaaatcgtg gcagcatatt ggaactgggt
      901 tgtaaatcca attgattaca aaaaatatag agctttcacg tatttatcag gaaatatctt
      961 ggatgacgta acccactgga tgccgctacc agaaccgccg caggaggtga agtgatggac
     1021 tattcacagt taagtgattt tgaaattaac agaatggtag gagacataat ttttaaaggc
     1081 ctttgggcgt gtaaaccgga aacatcaggg aataacacca acaaatggta ttacggaaat
     1141 actgatacaa cttttgagcc attaaaccct ttacctgact actgcaataa tccatcggat
     1201 gcatggccta ttattgttga taataaaatc agtctaacct gccaccaatc tcgcggtgag
     1261 tggtcagctg tttttaatgt cgagaacatt tgctttcatg cgaataatcc actccgagcc
     1321 gccatgattg tatttctcat gatgcaggaa aatcagaatg gctaaatcag cagcagagcg
     1381 caaagccgct cagagagcca gacaagctgc atctggtgtg cgtaagctgg agattgtgct
     1441 tgatgctcag gaaattgaaa tgcttgagcg taactgtgcc gcacgtcgcc ccgggcgtgc
     1501 gccttacgaa tttggtgagt atatagcgtt actgatccgc caggatgatg cacgcgtgcg
     1561 cgggcgtata aaatcgatca gtagaaaacg ttgcggtaag tgcggcgaga gagttcccgt
     1621 taattcatgc ccgtgtaatg gtgactcgca atgctgggtg actaaaggct ggtatgaaac
     1681 gaaattaata gtgtgacatg tcacgagtag attatgcatg atgaatttga cgggttttga
     1741 atactgccgc caactatggc ggctttattt tgcatggtac tattaccaca acggtaacta
     1801 ttaccacggt ggttatgatg cctgctgaac ctaaaaccta taaacgcaaa tcaacgcaat
     1861 ttaagccact aacagcaatg caggaggctt attgccagtc atacatcaaa acgcctgaaa
     1921 accagactca ggcagcgatt aacgcaggat tctccccaaa tacagcggca gttaaagcca
     1981 gtgtcatgat gcgcgatgaa cgcattcaga aacggattgc cgagttgatg gaggagcgca
     2041 acaaacgaat gcgcgtcagt gctgattacg ttctcatgcg cctggtggag atcgaccaga
     2101 tggacgttat cgacatcctc aacgacgatg ggagccttaa accaatccgt gagtggccga
     2161 aaatctggcg cactacgctt agtggctttg atctgtcatc gaccatcatg aacatgaacg
     2221 aggattcgat agagacaatc ctcaaaaaaa ttaaatggcc tgacaaggtg aagaaccttg
     2281 agctgattgg taagcatgtt gatgtcaatg cgttcaaaga acgtctggat gttaatgtga
     2341 atgtgacaat tgctgatcgc atagcggcag ccaggaagag actgaaagaa cgtcaggatg
     2401 gcaatcagtg acagatacag cgttatctcc tgaagagcag ttaattgagg atattgcagg
     2461 gttcactcac gatccgcttg gctatgccct ctatgcgttc ccgtgggggg aagaggggac
     2521 tgaactggca catgctaccg gcccacgtca gtggcaggcc gatgcgttcc gagagatacg
     2581 tgatcacctg cagaatccag agacgcgtta tcagccgctt atgctggcac gtgcttctgg
     2641 tcacggtatt ggtaaatccg cattcatctc aatgctcatc aactggggca tgtccacttg
     2701 cgaggattgt aaggtcgtgg tgaccgccaa caccgacaac cagctacgaa cgaagacctg
     2761 gccggaaatt atcaagtggt cgaaccttgc tatcacgaaa gactggttta cctgtaccgc
     2821 taccgcgatg tacagcaatg accctgggca cgacaagcgg tggcgggctg acgcaatacc
     2881 ctggtctgag cacaacactg aggcattcgc cggactacac aacgagcgca aacgcatcat
     2941 cgtggtgttt gatgaagcgt cgaacattgc ggatctggtg tgggaagttg ccgagggtgc
     3001 gcttacggac gaagacactg agattatttg ggtggcgttc ggaaacccta cacgtaacac
     3061 cgggcgtttt cgcgaatgtt tccgcaaata taaacaccgc tggaaaactg cgcagattga
     3121 cagccggacg gtggaaggca ctaacaaaca gcagttgcag aaatgggttg atgactacgg
     3181 ggaagacagc gacttcgtta aaatccgtgt gcgtggcata ttccctgatg catctgaatt
     3241 gcagtttatc cctaccggtc ttactgacga ggcaatgaaa cgggtggtaa ccgctgcgca
     3301 ggttgcacat gctccggtga taatcggcgt tgacccggca tactccggcg ttgatgacgc
     3361 tgtgatatac ctgcggcagg ggctacacag taaggtgctg tggactggca acaagactac
     3421 cgacgatctg attatggcga agcgtatcgc tgactttgaa gaccagtatc aggctgacgc
     3481 ggtgttcatc gacttcggtt acggaaccgg tttgaagtca atcggtgacg gctggggtcg
     3541 tacatggcaa cttgttccgt tcggtggcgc gtctactgac ccgcagatgc tcaacaagcg
     3601 tggggagatg ttcaactcat gcaagacatg gctgaggctc ggtggcatgc tggatgacca
     3661 ggaaacagcg gacgacctgt cggcagcaga gtacaaagtt cgagtggacg gtaaaatcgt
     3721 tatcgaaccg aaggaagata tcaaagagcg acttgggcgt tctcctggta aaggcgatgc
     3781 gctactgctg acgtttgcgt tccctgtgtc gaagcgtctg cgaattcccg gtcagcagaa
     3841 ccagcaaggc aaggccatca cagattacga tccctatgct taatccgctg gtggggataa
     3901 tgtcgttgat atcctctggt gaggataaaa caaagccagc tcataggctg gctgtttgtg
     3961 acatgtcaca gagctagaaa ataatttcat caaatgcagc atttattgcc tttgcttcgc
     4021 taatggcgcc ttgtttatcg acaatagctt ttttaccaag cacctcggca aggcactgta
     4081 gcttcatgtt gtataagtgt tcgctcatgt aatcattatc actttttgct ttagcgcaaa
     4141 cagagcgaga aatatcaaac acatcgcctt tttccatttc agaacgttgt gctgttatcc
     4201 agtcatggaa cgtaacgcta gtaccatggt cttcgctaag agttaacccg gtcagtccct
     4261 cactctgaat tacttcgtaa tgcatttcat taccagcaaa aacgccataa aaaacgcagt
     4321 cttcagcctg aacgatacga gaatatttta atggccattc atttagatac ttagccaaca
     4381 tatcaattgt tttcatgatc tcaccttaaa aaaatgcccg gcgaaccggg cgaactggaa
     4441 gcaatgagtt atgccttccg tggctgtacg ggtttacagc atgaagtcat cgcaatggcg
     4501 tcctgctgta aaaagggcgg tgatagtcct tcaagggaaa ccatcaccgc caagcacctg
     4561 gaacttctgg catcacggtc cttaggcgtg attctggcgc ggcatgcagg attcgaacct
     4621 gcgaccaacc gcttagaagg cggttgctct gtccaactga gctaatgcca caacgctgag
     4681 agcacttagc ctgttaaggc gccacacttt gtcgcggctc cataaatgct ctcatcgttg
     4741 taccctcgtc tcttccgagg tgtcacaccg aatcgccggg atggtgaatc cccgtgcgcg
     4801 gaataaaacc gctcgacttg cacattccgg ctacctggtt cgtttgcccg agcaagggag
     4861 ggtgcccctt aaacgtatcc agaccgctat cggcgcatgt gccatacgcc gtactgctca
     4921 aaataaaagc tcaccccacc tgttcaattt aacgacaagc cagtcagatt agtaactgga
     4981 gtgaattctt tggttacctg aaaggtaata attcgtgcgt taaatgtcaa ctgtctacga
     5041 taaataaatc atatgtggtt aaattggtaa taatttaatt gcgtacggag tcattgatat
     5101 gtgcatgggt agctcaccat cagtgcctgc aacaccagaa gttcaggcag caccacagga
     5161 gcaggatgcc gccgttgttg atgcccgcga cgaagaaact cgtcgccgtc gcgctgctgc
     5221 tggtcgtagt tctacgctgc ttaccggttc tcagggcgac acatcaaccg ctaataccag
     5281 cggtaaaacg ctgcttggtc agtaaccgga gtcattgaaa tggcggaaac aactaaagag
     5341 cgattgaaca aacagttcgc acaacttgaa agcgagcgtc agtcgttcga gccgcactgg
     5401 cgcgagttga gtgattacat caacccgcgt ggttcccgct ttctgacttc tgaggtcaac
     5461 cgtaacgatc ggcgcaatac acgcattatt gattcgaccg ggactatggc ggcgcgcact
     5521 ctcgccagcg gcatgatgtc aggcatcaca agccccgcgc gtccgtggtt tcgcctggct
     5581 acgccagatc ctgaaatgat ggattatggc cctgttaagt tgtggcttga ggcagttcag
     5641 aaccgcatga acgatatgtt caataagtcg aatctctacc agtctcttcc gcagttatac
     5701 ggaagcctcg gcacatacag cactggtgca atggcagtgc tggaggatga cgaggacatc
     5761 attcgcacaa tgccattccc gataggcagt tactacctgg ctaactcacc tcgtggcagt
     5821 gttgacacct gttttcgcaa gttctctatg actgttcgtc agcttgttca ggagttcggg
     5881 ctaaataacg tcagcgaatc cgtaaaaagc atgtgggaaa gcggcaccta cgagaagtgg
     5941 atcgaagtga tgcattcggt ttacccgaac attgaccgcg atacatcgaa gctggatagc
     6001 aagaacaagc cattcaaatc ggtttattac gaggttggtg gagataacga caagttgttg
     6061 cgtgagtccg gattcgatga gtttccaatt atggctccgc gctgggaagt taacggcgaa
     6121 gatgtttatg gatcatcatg cccgggtatg ctggcgcttg gacctgttaa ggcattgcag
     6181 cttctccaga agcgcaagtc gcagttgatt gataaagcca ccaatccgcc gatggttgct
     6241 ccgacttccc tcaagaatca gcgcgcctcc cttcttcctg gcgacatcac gtatatcgat
     6301 cagattactg gtcaggatgg cttcaggcct gcttatctgg ttaaccccag tacagcagat
     6361 ttggtggcag acattcagga cactcgtcaa atcattaaca gcgcctactt tgtcgatctg
     6421 ttcatgatgt tgcagaacat caatacccgc tcgatgcctg ttgaagcggt gatcgaaatg
     6481 aaagaagaaa aacttctgat gttggggccg gttctggagc gtctgaacga cgaatgtctt
     6541 aatcctctca ttgaccgcgc tttctcgatg atggtgcgca aaaacatgct gccgccaccg
     6601 cctgacgcga tggaaggcat gcccctgaag gtcgaataca tttccgtcat ggctcaggcg
     6661 cagaagtcta tcggcctgtc cagtctggcg tccacggtta acttcattgg tcaacttgcg
     6721 caagcgaaac cagaagctct cgacaaactc aacgttgatc aggcgatcga tgcattcgct
     6781 gatatgtccg gagtgtctcc aaccgtcatt gttccgcagg aacaggttga gcaggctcgc
     6841 cagcaacggg cacagcaaca acagcagcaa caaatgatgg cgatggggat ggcggcggca
     6901 cagggggcca agacgctaag cgaagctaaa acttcggatc cgagtgtgtt gtcagctatg
     6961 gcgaatgcag ttagtggtca gggtgggcaa tcacaatgac agattacgaa gacgatcaac
     7021 tgaaagaaga aaacgcccgt aagcaacgtg acatggcgca gcgtgaaatt gatgacattc
     7081 gctttgtcat gagcagtgaa caggggcgtc gcgttgtctg gtcggtgctg gagaaaggcc
     7141 gtgtgttttc cgctatctca ccgatggacg ctatggcaat ggcatttaat gaggggcaac
     7201 gcaatctggc gctggaactg tttcagcgcg ttatggcgca ttgccctgaa cagtatttga
     7261 agatggccaa agaggccagt gaacaggagt gatcatgaat ttatttgagc gtttgctgta
     7321 tcgccgtctt tgcaatgagc aaccagtcga tggtggagca gctccggctg cgtcagaacc
     7381 gtcagcgcct gcaggtgata accatgctcc agttggtgat ccatcacaac cggaaggtga
     7441 taagtcacaa cctgttgctg atggcgataa acctgctgat gacaaaaatc ctgaaaacga
     7501 taagcaggat gaaaaaaagg acggcgataa accagagggt gcgcctgaga agtacgagtt
     7561 tcaggctgcc gaaggcgtag agctggatac agaagcgttg aaggaattcg agccggtggc
     7621 gcgagaacta aacctgacca acgagcaagc gcaaaagctg gttgatgctt atccgaagat
     7681 tctggcaggt gttcagcagc gccaggtaga agcctggcag aaaacaaccg agcagtgggc
     7741 tgcggatgta aaagctgaca aagaaatcgg tggcgacaag ttgatttcta accttagcgc
     7801 cgcacagcgt gcgcttgacc agttcgggac acctgaactc aaagaatatc tgaacaccac
     7861 cgggctgggt aatcaccctg atctggtcaa aacgttcgtg aaaatcggaa aggcgatgtc
     7921 tgaagatggc atggtcaccg gtggtaatga aggccagcgt agtgcggccg aagtgctcta
     7981 tggcaaataa gagaggaaat gacaatggct gttaaaggct taactgcgct aacgctggct
     8041 gactggggta agcgcgtcga tccaaacggg aaagtcgata agattatcga gcttctcggt
     8101 caaactaacc cgatccttca ggatatgcct tttgtcgaag ggaaccttcc taccggacac
     8161 cgaaccacca ttcgttctgg tttaccttca gctacctggc gtttgctgaa ctatggcgta
     8221 cagccaagca aatcaaccac agtgcaggta accgattccg ttggcatgct ggaaacctat
     8281 gcggaagtcg ataagtcact ggctgatctg aacggcaata ccgccgaatt ccgcctgtct
     8341 gaagaccgcg catttattga agcgatgaat cagcagatgg cgcagacgct gttttatggt
     8401 gattccagcg ttaaccctca gcagtttatg ggactgtcct cccgctattc cagcctgtct
     8461 gcgggtaatg ctcagaatat cattgatgct ggtggcacgg gtacagataa cacctcaatc
     8521 tggttagtgg tgtggggcga aaacaccgtg catggcatct tcccgaaagg gcagaaggct
     8581 ggcatccaga tggaagataa aggccaggtg acactggaag atgctaatgg cggcaagtac
     8641 gaaggctatc gcacccatta caaatgggat aacggacttg ctctgcgtga ctggcgttat
     8701 gttgttcgca ttgcaaacat cgatgtcagc aatctttcag aaccatcctc tgccgcaaat
     8761 attgcgaagt tgatggttaa agcactacat cgcattccaa accgtggcat gggccgcccg
     8821 gtgttctaca tgaaccgcac tgtaggccag gctcttgatc tgcagtctct ggagaaaaca
     8881 tctctggcta tcagcgtaaa agagacagaa ggcgagtggt ggacttcatt ccgtggtgta
     8941 ccaatccgtg aaactgatgc gcttctggaa acagaagccc gcgtggtgta acgcctgtta
     9001 ttaacctgtg ggtcgtaaca gacccactaa tggagaaaga agatgatcac cgacaaactg
     9061 ttgatgttct ccgaagctca ggcggttacg aataccgcgg cttctactga cgtaatcgat
     9121 ctcggtccaa ttgacggaaa acgtcgtgat atcggcgtgg gttacccgct tgagttttgg
     9181 gtgctggtta acgcagccgc cgcagcaagc ggtgatgcaa ctgtaaacat ccagttgcag
     9241 acgagtgaga ataacacctc atggaccact atttatgata gtggcgcact ggtaaagacc
     9301 gccctgacag caggtaaacg agttgtttct gcaaaggtgc ctgccggtgt tcagcgatat
     9361 ctgcgtgtta actactccgt cgcaactggc ccactaacgg ctggcgaatt cactgctggt
     9421 atcagtcttg atgttgatgc caatacgccg tatccgatcc gctcaaaagt aactggttaa
     9481 ggtgatatcg atgtcaggtg agaaaccaag ataccgcgtt ctgcgcctct ctcatatcca
     9541 taacactctg tggccggagg gggcagaaat cgaatacgaa ggtgagcctg gtagcgcact
     9601 ggaacctgtt aacgatgcag ccagacaggc aaaagcaaaa gttgcaggaa aggtgtcaat
     9661 ggcagcaacc agcaccaaaa tcatcaacga tgtgtcagat gatggtgaac tggataagct
     9721 ccgtgaagag tacgaattgc tctttaacga gaagccacac cataacgcca aagctgaaac
     9781 gctccgcgag aagatcgcag ataagcgtaa agaactgggc gtgtaagcct cgcgaatcag
     9841 acaaggggct tcggcccctt tattgcagga gtgtatatgg aactcgtaaa cctcaaaacc
     9901 ggcactgaca gctaccagga tgagagcgga gaaaccagaa ctcgtgatga atacccgtgg
     9961 gggctgtgca tcactcttaa taacgacaca ttgaataagc tgaaggcgca acctaagggc
    10021 gtcggaacgg aagtgatgat aactgcaaag gctgttattc gaggcctgtc tgccagagaa
    10081 actgacgatg gcgttaatcg cagcgccgat ctgcagatca ctgatatggc gatcgctcct
    10141 gtttccgggg atgtagaaaa atcagcggct gaaactctgt acggtaacgg aggtgagtga
    10201 tggcctctgt agtagagatc tgtaatcgtg cgctgtccaa tattggcaac agccgcagca
    10261 ttaacagcct gacggaagcc agcaaggaag cgggggaatg ttcgctgcac tttgaggcct
    10321 gccgtgatgc tgtgctttct gattttgact ggaactttgc taccaaacgc gtggcgcttg
    10381 cagatacgag caatccaccg cctgactggg aatatgcgta ccagtacccg tcagattgtc
    10441 tgcgcattac tgaaattatg cttcctggtg tacgcaatcc aacagcagca atgcgcgttc
    10501 agtacgaagt tggtgcagac accaacggaa caggaaagtt gatctacaca gaccagcctc
    10561 aggcatggct caagtatgtc tctcgcgtta cagatgtgaa catgtttgat gccattttta
    10621 tggaggcgtt ggcctggcgt cttgcggcag ctattaacat ggcgctgact gggaatgcag
    10681 acctcggtac gtttgccctc aatatgtaca atcgcgtgat tcttagtgct ggctcgcata
    10741 gccagaatga atcacaggaa ccacagccac cggttgacga gtttaccatt gcgaggttgt
    10801 cctgatggct atcagttgga tccagcccag ctttgccggt ggtgagattg gaccgtcgtt
    10861 gtacggacgt atcgacatgg cgaagtacca ggtggcattg cgcaagtgcg ataactttat
    10921 cgtgcggcag tatggcggcg ttgagaatcg acctggtacg cgttttgtcg gtgccgccaa
    10981 atacccaaat cggaaatgcc gcctgatccc gttccagttc tcgacggttc agacctatgc
    11041 tctggagttc ggacaccagt acatgcgcgt tatcaaagat ggtgcgttgg tgctgaacag
    11101 cagcaatgtt atttatgaaa ttgccacgcc atatactgaa gccgatctgt tccgaattaa
    11161 atttacgcaa agcgccgacg tgcttacgct ggttcacccg gcatacccgc cgaaagagtt
    11221 gcgccgctat gcgcatgaca actggcaact ggttgatgtg gtaacgaaga acgggccatt
    11281 tgaagatatc aatattgacg agtcagtgac ggtttatgcc agcgccagca ctgggacaat
    11341 tacgttaacg gcaagcgcct ctatttttgg cgcggagcag gtaggcaaat tgttctatct
    11401 ggaacagcct gcagtggatt ctgtgccggt atgggaaacc agtaagagta cgtcgattgg
    11461 cgatattcgc cgtgcagaca gtaactacta tcgcgccgtt acagcaggaa aaacaggcac
    11521 tttgcgccct tcgcatacag aaggcacatc atgggatggc tggggcggat ccggtgatga
    11581 tgatactggc attgagtggg agtatctgca cagtggtttt ggcattgccc gtataactgc
    11641 tgtaaatggc actactgcaa ctgctgaggt gatttcctat atcccttcgc aggtcgttgg
    11701 cgaggataat gccagctata aatgggctaa atatgcctgg aacagtgtta atggttatcc
    11761 tggcactgtt gtttattatc aacaacgtct ttacttcgcc gcatcgactg cgttccctca
    11821 gactatctgg gccagccgta ctggggatta taaggatttt ggcaaaagca atcctacgca
    11881 ggatgacgac agaattatct acacctatgc cgggcgtcag gttaatgaga tccgccacct
    11941 gattgatgtt ggttcgctgg tggcgctgac ttccggaggt gagtatgtca tcaccggcga
    12001 ccagaacaaa gtgttaaccc catcatcatt tgcattcagc tctcagggat caaatggctc
    12061 aagcaacgtc ccgccaattg ccgtggcgaa tattgctttg ttcgtccagg agaaaggcag
    12121 tgttgtacgt gatctggcct actcattcga tgttgacggc tatcagggga acgacctgac
    12181 catccttgcc aatcatcttt ttcagaagca cagcattgtt gactggtgct tctcgattgt
    12241 cccttactcc agcgccttct gcattcgtga tgacggtaaa ttgctggtga tgacctattt
    12301 gcgtgatcag caggtttttg catgggcacc acaatccagt accgggaaat atgaaagcac
    12361 atgcagtatc agcgaaggca atgaagatgc ggtgtatttc gtcgttaacc gaaccgttaa
    12421 cgggcaaaca gtgagataca tagagcgact gtccagccgt ttatttacca gcgatgaaga
    12481 tgctttcttt gttgattctg gccttagcta tgatggaaga aatacgtctg acagaacgat
    12541 gaccatcact ggtggttctg gtgaatggga ttaccgcgcg gaatatacaa tcagtatttc
    12601 tggtggtgcg tacttcacca gtagtgatgt cggtgcgcaa ctacaattcc cttataccgg
    12661 aactgatcct gatactggcg atgaagtgtc aaaagaatta cgttgcgaca ttatttctgt
    12721 aaccagcaat accgctgtag tggttcgtcc taacaggaac gtcccatcat ccctcaggaa
    12781 tgtggccacc acgaactggc agatggcgcg ccggacattt ggaggcctgt ctcatcttga
    12841 aggccagatc gtaaacattc tctctgatgc gaacgtggaa ccacagaaag ttgtttccgg
    12901 aggtgccgtc acgctggaat ctccgggggc tgtagtgcac atcggcctgc caataactgc
    12961 tgaattcgaa acactggata tcaacattag cggacaggaa acgctgctgg acaaaaaaca
    13021 ggtgatcccg tccgttactc tggttgtgaa tgccagtcgc ggcatctggg cgactacgcc
    13081 cggcggtaaa tggtacgaat atccacagcg tgaattcgag ttctacgatg atcctgttga
    13141 tgatgctacc ggaaaagtag aagtgaaact ggacagtaac tggggcaaaa acgggcgtgt
    13201 aaaaatccgt cagcttgacc cgttgccgct gtctgttctt gccgttattc ctcgccttac
    13261 tgttggggga ttctgatgat cgatgttcaa attattcccg ctaccgaaga gcatcttcag
    13321 atgattttgc cggatgttcg tcaggctgat attgacgaac tgtatgcggt atcgctgatg
    13381 actaccgaag atgcgctgcg cgttggtctt cgcactgcga ctatggcctg gtcagggttc
    13441 gcgaacggag aactggtaac catgtttggt gtatctccgg cgtcaatgat cggtggcaat
    13501 ggtacgccct ggctggtagg aaccagccgt attgaaaaat atcagaagac atttctgcgc
    13561 cactgccgcc ctgtattgca gcagatgctg gcagtttatc cgcgcctgga aaactacgtc
    13621 gacgagcgaa accatgttgc caaagcatgg ctgcactggc ttggattcag gcttgaagaa
    13681 gccgcgcctt atggtgctct tggtcttaat ttccacagat ttcacatgga gagaaaataa
    13741 tgtgcgatcc ggttattgct ggtggcgcaa tgctcgccat gagtggcatt caggcataca
    13801 cccagtacca acagggaaag tatgcctcga aggttgcaga agcgaacgca gatatagcca
    13861 cagctcaggc aaatgatgca ataaacagag gtaacgctga agctgagcaa cgacgcagag
    13921 agacccgaca gcggcttggt acacaggcgg cgacaatggg ggctaccggc gctgatttat
    13981 ctacaggtaa cgcgctggat atatttggcg acactgccca gtttggcgct cttgattcgc
    14041 tgacgacggt gaataacgcg caacgcgagg cttacggtta tcaggttcag gctgccaact
    14101 ataaagcaga agccagttca gcccgtaaac aggggaatgt gggagcagca acaacattgc
    14161 tcactgcgcc tctgaaggca tacggtgcct accagatgtt tggtgggacg tggagtccgt
    14221 ttactcaaag tactcctgca ccaatcgggg cagcagcagg aaccagatta cccggaggat
    14281 tataatgcca gtcgtaccaa cagtatccgg acgtcaggtt gagagccgtg gagttcagtc
    14341 agcaggcttg cagacgtttt ctcagccagg tattggtgat gcttttgttc gggcaggggc
    14401 agaggctatt gatgtttttg gtcaggcaaa acagcgtgcc aatatcgctc tggctcagga
    14461 ggcatctctt aacctcagtc agataagcag tgatctgctg aataatcctg aaacaggatt
    14521 gcttaacctg aaagggaaaa atgctattgg aaaaggccat gagtatacgc agcagtttga
    14581 tgctcaggtc gaacaactgg ctatgtcgct gccggatgaa caggctcgta atgctttcat
    14641 gcagcaggcg cagcagcagc gcattcagtt cactacgcag gcagggcggc acgagatagg
    14701 gcaaataaat gcctacgaag aaggccagtt tcaggctacg ctgctgaaca atggtaaaaa
    14761 tgccgcagca ttgtatggcg acaacgccgc atacgtattg gctaataagc aaactttcca
    14821 gcaaattgag gattacggca ttgcacatgg ctggagtgac gagcaaatcc aggccaagaa
    14881 aatcgagttt aaagaagcaa cagcaaaagc aactgctcaa aatgctattg gagcaaacta
    14941 tcttcaggta agacagcaaa acggcgagtt aagcgatact gctgctggat ctcgccgtgc
    15001 tgtagcagat agtggctctt tcgatcgtac ccgcggtata cgcaacaata accccggcaa
    15061 tcttgaatac agcaaaacta atccgtgggt tgggcagaca ggtgatgatg gtcgctttgc
    15121 taaattcgaa acacctgaac acggtattcg tgcattaggg cggaacctga tgtcgtatca
    15181 gcggcagggt attgataccg tcagcgagat aattaatcgc tgggcaccgc ccactgataa
    15241 aaatgacaca atgtcgtata tcaaagcagt gtgcgaacaa cttggcgttt ctgctgatga
    15301 gtctctcgat gcatctaatc cggataccct gaaggcgctt tgtgcagcca ttatccatca
    15361 tgaaaacggt agccagccat acagtgatca gcagttaact gatggtgtta gtgctgcgct
    15421 ggggctttct cagctaccga caaaaaataa acgttatacc ggtgtagctt ggttcgatgc
    15481 tttaagtgaa tcagatcagg ccagcgtgtt gcgacagact gatgcactag ccagacaaca
    15541 gcaggctgaa tataaaacga tgctcgacag ccgggttcgc gatgcgacgg ctgcgtatat
    15601 gcgtggcgtt gaatttccta acccacctgg tgaggatgat tttattgcag cttatggagt
    15661 cagagaagga aacctgcgat ataccgagtt taagaatacg cagatcgccg gacagtatat
    15721 aggctctttc cgcaacatgc cgacaagcag cattacagca tatgttgagc aattacgccc
    15781 ggatactggt gatacagggg aggggtatgc ggcacgcgca gctctttatg acaacgttgt
    15841 ttcggctgca aatcaggtga taaagcagcg gcagtcggat cctgtgcagt tctctcttgc
    15901 ctccggacag gcaaagccta tcgacatgag caataaggat aactttggac agagcgttgc
    15961 cttgcgtgcc gctcaggtca gtgaccttgc taagtcatat ggcactccac tgacgttctt
    16021 ttccaaagac gaggccaatc agatcggtgt tttctttcgt gatgctccag tttcccaaca
    16081 ggcagcatat ctcgatacca tcaggcagag cactggtggt gggcaggtgt atatgtcagc
    16141 actacagcag atcagtgcca acgctccatc tgctgccgtt gccgggatac tgatggataa
    16201 gcctggtggt attttggcag aaaaaaactg gtttaatccg gatgtttccg tgtctcctga
    16261 aaccgctgcg cagacaattc ttgctggcgc ggcggctcgt aaaggtactg atgatgcgaa
    16321 aggtattccg atgcctaaag atgctgatct tcgccttgag ttttctgaca tggtgaagga
    16381 tgcatttgct ggtgacgctc agggggcatc aatggcatac gagatcgcaa aggattatta
    16441 cgctggtgtg atggcgaaaa aaggcgtggt atcaggcgaa attgacaatg atgtctggaa
    16501 acaggctgtt aacgtagcta caggtggcgt gcatgactat aacggaatgg ggaatgtcct
    16561 tttgccgtgg ggaatgtctg cagagcaatt cgataagcag gttaatcagg cttggaatga
    16621 acaagttgtc ggctctggga taaaaacacc gcctggtcag tatggtttgc aaagttacgg
    16681 cgatagtcag tacctggtga aacttggtac tggttatctg ctgaaagatg atggttctcc
    16741 cgttgttctt aatctgacac agaagcgtca gagattctcc ggagatattc cgcaatgagt
    16801 tactttggcc ttaatccagt aaaccagaat cagcagcttg acgaagcagc atcaaatcca
    16861 gctggcttta acagcgatgt tggttttttc gacaatgctg taggagcggc attgtctggt
    16921 ttgtactccg ggctggtggc aaagccagat cagttgctat gggcagggat ggataaaatc
    16981 gtatccccga ttgctcagtt tgttaacgaa aacacctcgc tcaatgatac ttcagtttca
    17041 tacattgccg agcagagaaa actagcagag cagcaggtta agcggctgac gcctgatgcc
    17101 gcgacaaccg gaaccgctgg gcaggttctt tatgggttgt tcgatatggg cgggcaggct
    17161 gttgtcggta caacgctcgg tggtcctgtc ggaggtgcag cggcggtaac ttcgctacag
    17221 ggtttttctg agtttgaacg gctgacagca cagggtgttg atttcaggac ggcgcaggaa
    17281 gcgggattag tgcagggcat tactgctggt gccggaacgc tgatccctat gagcctcggg
    17341 ttacgtgctg gtggtgcgct ggcggaaggt gtggcggctc agcttgcgcg gacgggtgag
    17401 agttcagtgc gacgcgccgc agcaacagca gtacgtgcaa cgccagatat tgcctatgcc
    17461 gcaggtacaa atattgcgtt cggtatggca cagcgtgggc ttactgcaaa aacacttcgt
    17521 gatggtggct atagcgaaat ggctaaccag tatgatgtgt tggatcgaca ggcaattgct
    17581 attgatgctg ttcttggggt ggcgtttggt ggtgtcggca gatttattaa ctctcgcggc
    17641 gagtctacaa acgcaccaaa tttttcacca gttgatatcg atgctgcact ggcggcgaat
    17701 gccgctcatc atgctgaaat tgatattgcg cccggcgtgc cgatcaacgt gctttcgcgc
    17761 aattctcaca ttcaggctct gcgaaaagct atgtctgatg ttagccaggg gagacctgta
    17821 gacgttgcca gcattgttga gtctgcatct ttcagtgaaa ttcctgggcg caagagtctg
    17881 ctgtctcagg cagttaatga ggctctgtca tctgtagatg atggagtaac ggcgcgcgct
    17941 atagaaaatc ggttgcttga agaacaggcc gcgcagcttt tgccgcgtgg agatagacag
    18001 gtttaccagt ctgaaatcgc taatagccaa cgaattattg aaaatctcac tgaacagcgt
    18061 gcacaaattc ttgcagaaga gccaaccggt agcggtaaag ctttgtctcg tgctcgatca
    18121 gataaacagg ccagacttcg cgatattgat caacgaatcc ggcaggcaca agaacgcctg
    18181 gaattttccc gtaacgcgtt ggcaccgcac gagcctggcg gtcagttttt tgaagctcga
    18241 gcagaactgg ctcggagaca gcaggcagaa agtgaactta atgctcaggc tgtttcattc
    18301 tataaaacag cagaggtcag gacgccagac gaagtagctc cttttgagcc tgataaaata
    18361 ttgcaacagg cagaacaaaa aatgatgtca gatcaggcag gagatattga tctgcgcata
    18421 gctgaagact cgctgcttga atcacctgac atgataatca ccgtgctgga tgatgatggt
    18481 aatccacaat cgcgcagtgc gcgtgaagta ctggatgaag cgaacaggga aagtgagcag
    18541 gcaatacagg attccagcct gtttgatgtt gctgtggcgt gtttcttgag aggttaaatt
    18601 aaatgagaca ggaatgtata caagcggtcc agcaggcggc gcagcgcacg ttaacggcgc
    18661 gagaaataca gaacattgaa gaccgcattt atcgaaatat gcgctccatt gctcgtgatg
    18721 acccgatgtc gtggcgacaa ctttccgaat cagaacggtt gtatcgtgca gcacaattgg
    18781 catctgaaga attacagcga gaagcggcat taaagaaacg tcgtgtggcc ctcactatag
    18841 ccgcacgtca gagattggat aaatttatca atagctatca aggggctgat gggaaacttg
    18901 gcgctcttaa ccgtactatt gcttttaatg cagacggtaa atcgaatttc ctctctgttg
    18961 aatccagaac aaaagccacc cgtgattatg cattgagtca attgcaggag gcattcgaag
    19021 cagttgatcc tcgctttttt ggtctgtttg aagatgaagc gggcgtacgt gacctgatat
    19081 atgaaatgcg ggggcaaaat actggcaatg ctaaagcaag aaaaggtgct aaggcgtgga
    19141 gagaagttac agagctgctg cgccgccggt ttaatgatgc tggtggggac attggctatc
    19201 tcgaaaactg ggggatccct caacatcatt ctatggaaaa ggttggggcg gtatcaaaag
    19261 ataaatgggt tagcgatgtt ataggtaagc tggatcgcaa atattatacc cgagccgatg
    19321 gacaactgat gaacgatgcc gagttgtctg catttcttgg agaggcttat aacacgatcg
    19381 ctactggtgg gctgaataag cttactgata ccggaatgcg aatttccggc gcacgtgcta
    19441 accgtggtaa tgcatcacga cagatacatt tcaaagatgc agattcctat ctgcaatatc
    19501 agcaacttta tggcgatcgc tctctatggg aaatcatggt cggtcacctg gaaggtatca
    19561 gtaaagatat tgcactggtg gaaacatatg gtccaaaccc cgatcatgtt ttccgctctc
    19621 ttcttgatca ggtgaaggca gaaacggcaa cagctaaccc gagtaaaacc ggtaaagtcg
    19681 agcggctggc gaacaacaca gagaatctgt acaactttat ttccggaaag acacagcctg
    19741 tagcgaatcc gcacatcgcg cgatggtctg acaatatccg caactggctg gttgccagca
    19801 gactcggatc cgcgttgctg tcatcgttct ctgatcttgg aaccatgtat ctgtctgcga
    19861 aggttaccaa ccttccaatg aaccagttat tccgcaacca gcttgaagct atggacccaa
    19921 cgaaccgtac agaacttgcg cgggcgcgcc gcgctggtct ggcgatggaa tctctacttg
    19981 gcagcgttaa ccgctgggcg atggataata tggggccgtc tgtgtctcgt tgggctgcaa
    20041 cggcggtaat gcgtgccagt gggcttacag catggtcaga tgcgcacaag cgcgcctatg
    20101 gcgtaaccat gatgggaagc ctgggagaag tagtgtcacg gacaccagac cttcgtcgcc
    20161 tcgatgactc tgattttcgt atcctgaaaa gcaaagggat tactgacaca gactggagcg
    20221 tatggaagtt ggcgcaacag gaggactggg ggaacggtaa taatacgatg ctgacaccgg
    20281 aaagcattat gcgtatccct gattcagcag ttaaacatct tggtgagcct gaacgcgtga
    20341 aatttgaggc aatgcgtaaa ctgctcggtg ccgtaactga agaagttgat atggctgtta
    20401 ttacaccggg agcacgtgag caactgataa ccggttctgg tattcagcgt ggaacatgga
    20461 aaggtgaatt aacgagaagt gttttcctgt ttaaatcgtt ccctatctcg gttgttatgc
    20521 gtcactggtc acgcgctatg ggtatgccgt ctgctggtgg gcgtgcggca tatattgcga
    20581 cgtttattgc cagtacgacc attcttggcg ctttgtcgca acaacttaac gaccttgcgt
    20641 ctggtcgtaa tcctcgagag atgacaggag aagatgctgc taaattttgg cttggtgctc
    20701 tactgaaagg tggtggtctt ggcctttacg gtgacttttt attgtcagat cacactaggt
    20761 acgggagcgg cgcgctggcg tcgatgcttg gcccggtagc tggtctggtt gatgacgtag
    20821 tgaagatagc tcagggcata ccgctaaatg ctgtggaagg gaagagtgag cagactggtg
    20881 gtgatctggt gaagctgggg aaaggtttga tgccaggtgc gaatctctgg tacttgaagg
    20941 cggctctcga tcacatgatc tttaatcaga tgcaggagta tttttcacca ggctatttgc
    21001 gtaaaatgga gcaacgttcg aagaaagagt ttaaccagac atactggtgg cgacctcagg
    21061 atgtcactcc gcaataagga agtgttgtgt ttttaattat tttgagtgtg ataatttctg
    21121 gtggattgtt atttattgac cgctacaaat attttcttaa ccctcagact caagctattt
    21181 gctggttcat ctttgttgtg cagggagtag ttcttgttgc aagccttatt gaggggaggc
    21241 ctcttatttt tactgggtaa ataggtgact acatgcaagc tataggattc attgtttata
    21301 tcgtcgttgg tctttttcag ttggcagcaa ttatggctgg gcttgaatca tggtggggat
    21361 tgcactggat aattgcagcc cccattgctt tcatcgtgag ctatattcca tttgttggag
    21421 cgattgttgg tatggttggc gctgtggatg tatggcggtg ggagtggtgg caggctggcc
    21481 ttctcttctt tggtgggatc atctttgcta ttgtctgcgg tggaatgtca tcatttttcg
    21541 aatggctatc attcagaaaa gggacgtgac atgtcacagg ccgctttcgc ggccttgttt
    21601 ttaacgaatg ccaccgccgc ccgggcggga atccgcagaa cgcccaccgc agcgggagcc
    21661 gtcagcggca gtgtcgctgt cgtgctgaca acgaccggca aaggcctgag ttgaagctac
    21721 cagagacaac aaaacgaaca gtgcagcaaa tgcttttttc attgtgaaat ttccatctat
    21781 aagccacctc aatgtggcgt caatgagtgt agcactgact tttgtttcgt ccacaaaaaa
    21841 gcccgcagcg cgggctattc ttcttcatca ttaaaaagcg gattgttgtt tccatctgat
    21901 gaaaggaaag gtatgtgttc tcttgaaaat ccaacaatga caatttcatt atcatttttt
    21961 gcataatgaa tgcattcatt agaatgcctt ccgctagggt tgaaatttaa gttaatggta
    22021 taattcttaa atgtgtttgg ataccatgtt ggcccacaat gataatgcca ataattctct
    22081 tgctcataat tgtctgagcc gggaatttta tcatggttat catcaaccca tgagggcttg
    22141 ttttttccaa caagcgccct tccattggca acatcctcta aaaaactttg tatgattttt
    22201 atttcatcat cagtaaggaa aggtccgtct acagcgaaag gggtgttgct ttccccatgt
    22261 agaaaactat tagatattct aatgttaaac ttcaaggaaa actcctgatg tgcttctatt
    22321 tggagcgaaa tgccgccttg aagtcgctga aagatgttcc tgtctggtaa accatatcct
    22381 tgtcgcgctc tttgcttgcg cggccaagca taacttttcc aatgacatcc cagcattgct
    22441 tggcttgttc attgtgctga gtacgatttt tcaacgctgt catatcgcgc ctcctttacc
    22501 tttaaggtaa tattacgcga atctacatca catcgccaat gattatttta aaggcacatc
    22561 cctgtgccgc cgcccgtcag aagaacccag ccttgtcgtt gatgtactcc gcgtgggtct
    22621 ggatatcacg caggcatttg ctcacaccga cgatgtagca gaacatggtg gtcagctccg
    22681 ccgccgcgcc cgatacgtcg tgcccgtctt cctgtaactg gttcagcaga ttcatcagca
    22741 gtgagttctc cgtcaggccg agaacaccag acggcgaatg aatcaggcta cggtagccgg
    22801 gcttcagtgg ggcactgtag gttttgttct ctatcttcat cgcctgcatt actgctgacg
    22861 ccgtggcgtt ggctacctgg tcggcaacca tctttatgcg ttcttcctgc gggagcgagt
    22921 ttttaatgta acttccggtg cggcggatct gaggaagaac ctcacctgta acccatttac
    22981 gaaagcggta ggggatagtg cctggtgtca ctgcgtcgcg gcagcggagg atcagtgtgt
    23041 agaggcctga ctcggagatg atgttgatct cttttactcg gctgtcaaaa attgcacgat
    23101 gttcatgccc tatgttgaac atagaccttt catcatcatc cagtttttca agtgcttggg
    23161 tgacgttttg gatgcgcagc gcactacaaa cgtcttgggc tacaaaccat ggttggccat
    23221 cgataatgat ggaacggata gggttaacag attcaaattt gaagatggca gtttgagcat
    23281 tagccatggt ggttatctcc acttagtgat tttaatcacc accgcaacgc caattactgg
    23341 tggtgaactg gacaaggttg gcgtaccggc ctaagtggta ccggcgtcct ttcggacccc
    23401 tgcccagccc accataattc ggatatagct gtgcttaacg cataaaaaaa ccacgtctgg
    23461 cgtggtatgc gccacttagt aactcgggac gccaatcccg gcactggatt ttgccagtgc
    23521 ctgattacta tggcacaaga ggagtgcgat gtaaatttac cgcaaaggta aatataagca
    23581 ctccacttgg taattgcaaa ccttatctgg tttgctttcg taattgttcg gcacaatagt
    23641 cgagatgtgt ttgcagatcc tgcatagaca tctgtgagct ggtgacgtag ttaatcagtg
    23701 cagtcagttc ggcaagtggg ccatcgacat taaatccatc cttatcgaga tcccggagta
    23761 atttcatcaa atgcgatccc tccaccagtg acctgacgcc tcccggcgtg tgaatccttt
    23821 cggtaaatcc gtcttccagt ggatagtgat actgctgcat cttatcttct ccatgcaata
    23881 actgtatatt tatacagtag caaataattt gtttgctatc cagcacgttt tgcaaattac
    23941 ctgaaaggta atatctattc gtattcacag tctttctatc catatgtggt ttttcaggta
    24001 atagaataac cagatatgcg gcgcaacggg tgctgcgact atctggagat ttaacatgac
    24061 ggtctcaacc gaagttgacc acaacgaata caccggtaac ggcgttacga catcgtttcc
    24121 gtataccttc cgtattttca aaaaatcaga cctggttgtt caggtgtctg acctgaacgg
    24181 gaacgtaaca gaattggttc tggataccgg ttatacggta actggggcgg gcacttatag
    24241 tggcggttct gtggttcttc cgtctccgct tgctgctggc tggcgaatta cgatagagcg
    24301 tgtgcttgat gtggtgcagg agactgatct tcgcaatcag ggaaaatttt tccccgaagt
    24361 tcatgaagat gcatttgact acctgacgat gctgatccag cgatgttttg ggtggttcag
    24421 acgtgcattg atgaaaccat ctttgcttgc aaaatattac gatgcaaagc aaaacaaaat
    24481 ttctaacctt gcagatccat catttgagca ggacgctgta aataatcgct caatgcgtaa
    24541 ttatgtcgat gctgcaatcg ccggggttgt tggtggtttt ggttggttta taccgtcagg
    24601 aagcggggca agacaacgaa catttcaaag caaaatgagg gatataattt ctccttgtga
    24661 ttacgaagat aatattttta tgattaagga gttgatatcc aaaggcaaaa atgccgctgt
    24721 ttttaactca tattatactt ttgttattac tgttggcgaa tggggtgatt accctacact
    24781 caaagatgca atcattgcag cccagtcaat gagacctatg tgggatggtg gtaatgcttt
    24841 ctgcgaaata agaattaaga ccgggcatat tatcaactat caaattgaat tcagttcagg
    24901 tgttgatttg tcttggatta aaataacatc agaagactca gtggttttct ctgacactcg
    24961 tttgtttact aaaatcgtta gaacgtatta cgaatacaag tatttatttt acatttgcga
    25021 tgcagcaaaa agtcctgtat ttgctattca gatcgaagaa aatagagatg atagcgatgt
    25081 ttgtgcattt attgtaactc aaaaagctga acttaatttc tatccgtatt caggtgctcg
    25141 aaagttctac gttggaattc atggtagttt tgg
//
LOCUS       DAMZFT010000073        24908 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.073, whole genome shotgun sequence.
ACCESSION   DAMZFT010000073 DAMZFT010000000
VERSION     DAMZFT010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 24908)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24908)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24908
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.073"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            21..96
                     /locus_tag="PN977_002985"
     tRNA            21..96
                     /locus_tag="PN977_002985"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:54..56,aa:Ala,seq:ggc)
     gene            304..2493
                     /locus_tag="PN977_002986"
     CDS             304..2493
                     /locus_tag="PN977_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing phosphodiesterase"
                     /protein_id="HDI6043000.1"
                     /translation="MFVEHNLIKNIKIFTLAFTLTVVLIQLSRFISPLAIIHSSYIFL
                     AWMPLCVMLSILFIFGWRGVVPVLCGMFCTNLWNFHLSFLQTAVMLGSQTFVVLCACA
                     ILRWQLGTRWRYGLTSRYVWQRLFWLGLVAPIGIKCSMYLVGNFFDFPLKISTFFGDA
                     DAIFTVVDLLSLFTAVLIYNMLFYYLTRMIVSPHFAQILWRRDIAPSLSKEKRAFTLS
                     WLAALSVLLLLMCTPYENDFIAGYLVPVFFIIFTLGVGKLRYPFLNLTWAVSTLCLLN
                     YNQNFLQGVLTEYSLAFILAVLISFSVCLLYMVRIYHRSEWLNRRWHLQALTDPLTLL
                     PNFRALEQAPEQEAGKSFCCLRIDNLEFMSRHYGLMMRVHCIRSIYRTLLPLMQENEK
                     LYQLPGSELLIVLSGPETEGRLQHMVNILNSRQIHWNNTGLDMGYGAAWGRFDGNQET
                     LQPLLGQLSWLAEQSCAHHHVLALDSREEMVSGQTTKQVLLLNTIRTALDQGDLLLYA
                     QPIRNKEGEGYDEILARLKYDGGIMTPDKFLPLIAQFNLSARFDLQVLESLLKWLATH
                     PCDKKGPRFSVNLMPLTLLQKNIAGRIIRLFKRYHISPQAVILEITEEQAFSNAESSM
                     YNIEQLHKFGFRIAIDDFGTGYANYERLKRLQADIIKIDGVFVKDIVTNTLDAMIVRS
                     ITDLAKAKSLSVVAEFVETQQQQALLHKLGVQYLQGYLIGRPQPLAD"
     gene            complement(2542..3744)
                     /gene="nupC"
                     /locus_tag="PN977_002987"
     CDS             complement(2542..3744)
                     /gene="nupC"
                     /locus_tag="PN977_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupC"
                     /protein_id="HDI6043001.1"
                     /translation="MDRVLHFVLALAVVAILALLVSSDRKKIRIRYVIQLLVIEVLLA
                     WFFLNSDVGLGFVKGFSEMFEKLLGFANEGTNFVFGSMNDQGLAFFFLKVLCPIVFIS
                     ALIGILQHIRVLPVIIRAIGFLLSKVNGMGKLESFNAVSSLILGQSENFIAYKDILGK
                     ISRNRMYTMAATAMSTVSMSIVGAYMTMLEPKYVVAALVLNMFSTFIVLSLINPYRVD
                     ASEENIQMSNLHEGQSFFEMLGEYILAGFKVAIIVAAMLIGFIALIAALNALFATVTG
                     WFGYSISFQGILGYIFYPIAWVMGVPSSEALQVGSIMATKLVSNEFVAMMDLQKIAST
                     LSPRAEGIISVFLVSFANFSSIGIIAGAVKGLNEEQGNVVSRFGLKLVYGSTLVSVLS
                     ASIAALVL"
     gene            4080..5318
                     /locus_tag="PN977_002988"
     CDS             4080..5318
                     /locus_tag="PN977_002988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HDI6043002.1"
                     /translation="MTNYRVESSSGRAARKMRLALMGPAFIAAIGYIDPGNFATNIQA
                     GASFGYQLLWVVVWANLMAMLIQILSAKLGIATGKNLAEQIRDHYPRPVVWFYWVQAE
                     IIAMATDLAEFIGAAIGFKLILGVSLLQGAVLTGIATFLILMLQRRGQKPLEKVIGGL
                     LLFVAAAYIVELIFSQPNLAQLGKGMVIPSLPTSEAVFLAAGVLGATIMPHVIYLHSS
                     LTQHLHGGSRQQRYSATKWDVAIAMTIAGFVNLAMMATAAAAFHFSGHTGVADLDEAY
                     LTLQPLLSHAAATVFGLSLVAAGLSSTVVGTLAGQVVMQGFIRFHIPLWVRRTVTMLP
                     SFIVILMGLDPTRILVMSQVLLSFGIALALVPLLIFTSDSKLMGDLVNSKRVKQTGWV
                     IVVLVVALNIWLLVGTALGL"
     gene            complement(5386..5712)
                     /locus_tag="PN977_002989"
     CDS             complement(5386..5712)
                     /locus_tag="PN977_002989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein"
                     /protein_id="HDI6043003.1"
                     /translation="MFRSLFLAAALMAFTPLAANAGEITLLPSIKLQIGDRDHYGNYW
                     DGGHWRDRDYWHRNYEWRKNRWWRHDNGYHRGWDKRKAYERGYREGWRDRDDHRGKGR
                     GHGHRH"
     gene            complement(5827..7083)
                     /locus_tag="PN977_002990"
     CDS             complement(5827..7083)
                     /locus_tag="PN977_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ion channel protein"
                     /protein_id="HDI6043004.1"
                     /translation="MLHPRARTMLLLSLPAVAIGIASSLILIVVMKIASVLQNLLWLR
                     LPGTLGIAQDSPFWIIAILTLTGIAVGLVIRFSQGHAGPDPACEPLIGAPVPPSALPG
                     LIVALILGLAGGVSLGPEHPIMTVNIALAVAIGARLLPRVNRMEWTILASAGTIGALF
                     GTPVAAALIFSQTLNGSSEVPLWDRLFAPLMAAAAGALTTGLFFHPHFSLPIAHYGQM
                     EMTDILSGAIVAAIAIAAGMVAVWCLPRLHAMMHQIKNPVLMLGVGGFILGILGVIAG
                     PVSLFKGLDEMQQMVANQAFSTSDYFLLAVIKLAALVVAAASGFRGGRIFPAVFVGVA
                     LGLMLHEHVPAVPAAITVSCAILGIVLVVTRDGWLSLFMAAVVVPNTTLLPLLCIVML
                     PAWLLLAGKPMMMVNRPKQQPPHDNV"
     gene            7287..8252
                     /gene="glk"
                     /locus_tag="PN977_002991"
     CDS             7287..8252
                     /gene="glk"
                     /locus_tag="PN977_002991"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HDI6043005.1"
                     /translation="MTKYALVGDVGGTNARLALCDIASGEISQAKTYSGLDYPSLEAV
                     IRVYLEEHKVEVKDGCIAIACPITGDWVAMTNHTWAFSIAEMKKNLGFSYLEIINDFT
                     AVSMAIPMLKKEHLIQFGGAEPVEGKPIAVYGAGTGLGVAHLVHVDKRWVSLPGEGGH
                     VDFAPNSEEEGIILEILRAEIGHVSAERVLSGPGLVNLYRAIVKADNRLPENLKPKDI
                     TERALADSCTDCRRALSLFCVIMGRFGGNLALNLGTFGGVFIAGGIVPRFLEFFKASG
                     FRAAFEDKGRFKEYVHDIPVYLIVHDNPGLLGSGAHLRQTLGHIL"
     gene            8471..8797
                     /locus_tag="PN977_002992"
     CDS             8471..8797
                     /locus_tag="PN977_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-like phosphotransferase enzyme IIB
                     component 1"
                     /protein_id="HDI6043006.1"
                     /translation="MSKKLIALCACPMGLAHTFMAAQALEEAAVEAGYEVKIETQGAD
                     GIQNRLTAQDIAEATIIIHSVAVTPEDNERFESRDVYEITLQDAIKNAAGIIKEIEEM
                     IASEQQ"
     gene            8819..10066
                     /locus_tag="PN977_002993"
     CDS             8819..10066
                     /locus_tag="PN977_002993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HDI6043007.1"
                     /translation="MAIKKRSATVVPGASGAAAAVKNPQASKTSFWGELPQHVMSGIS
                     RMVPTLIMGGVILAFSQLIAYSWLKIPADIGIMDALNSGKFSGFDLSLLKFAWLSQSF
                     GGVLFGFAIPMFAAFVANSIGGKLAFPAGFIGGLMSTQPTQLLNFDPSTMQWATSSPV
                     PSTFIGALIISIVAGYLVKWMNQKIQLPDFLLAFKTTFLLPILSAIFVMLAMYYVITP
                     FGGWINGGIRTVLTAAGEKGALMYAMGIAAATAIDLGGPINKAAGFVAFSFTTDHVLP
                     VTARSIAIVIPPIGLGLATIIDRRLTGKRLFSAQLYPQGKTAMFLAFMGISEGAIPFA
                     LESPITAIPSYMVGAIVGSTAAVWLGAVQWFPESAIWAWPLVTNLGVYMAGIALGAVI
                     TALMVVFLRLMMFRKGKLLIDSL"
     gene            10081..11166
                     /gene="ypdF"
                     /locus_tag="PN977_002994"
     CDS             10081..11166
                     /gene="ypdF"
                     /locus_tag="PN977_002994"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HDI6043008.1"
                     /translation="MTLLASLRDWLKAQQLDAVLLSSRQNKQPHLGISTGSGYVLISR
                     ESAHILVDSRYYADVEARTQGYQLHLLDATHTLATIARQIIADEQLKTLGFEGQQVSW
                     ETAHRWQSELNAKLVSATPDVLRQIKTPEEVEKIRLACGIADRGAEHIRRFIQAGMSE
                     REIAAELEWFMRQQGAEKASFDTIVASGWRGALPHGKASDKIVAAGEFVTLDFGALYQ
                     GYCSDMTRTLLVNGEGVSAESHPLFDVYQIVLQAQLAAISAIRPGVRCQQVDDAARRV
                     ITEAGFGDYFGHNTGHAIGIEVHEGPRFSPRDTTTLQPGMLLTVEPGIYLPGQGGVRI
                     EDVVLVTPQGAEVLYAMPKTVLLTGEA"
     gene            11166..12203
                     /gene="ypdE"
                     /locus_tag="PN977_002995"
     CDS             11166..12203
                     /gene="ypdE"
                     /locus_tag="PN977_002995"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HDI6043009.1"
                     /translation="MDLSLLKALSEADAIASSEQEVRQILLEEADRLQKEVRFDGLGS
                     VLIRLNESTGPKVMICAHMDEVGFMVRSISREGAIDVLPVGNVRMAARQLQPVRITTR
                     EECKIPGLLDGDRQGNDVSTMRVDIGARSCDEVMQAGIRPGDRVTFDTTFQVLPHQRV
                     MGKAFDDRLGCYLLVTLLRELHDAELPAEVWLVASSSEEVGLRGGQTATRAVSPDVAI
                     VLDTACWAKNFDYGAANHRQIGNGPMLVLSDKSLIAPPKLTAWIETVAAEIGVPLQAD
                     MFSNGGTDGGAVHLTGTGVPTVVMGPATRHGHCAASIADCRDILQMQQLLSALIQRLT
                     RETVVQLTDFR"
     gene            12228..14723
                     /gene="ptsP"
                     /locus_tag="PN977_002996"
     CDS             12228..14723
                     /gene="ptsP"
                     /locus_tag="PN977_002996"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HDI6043010.1"
                     /translation="MLTIQFLCPLPNGLHARPAWELKEQCSQWQSEITFINHRQNAKA
                     DAKSSLALIGTGTLFNDSCSLNISGSDEEQARRVLEEYIQVRFIDSDSVQPTQAELTA
                     HPLPRSLSRLNPDLLYGNVLASGVGVGTLTLLQSDSLDSYRAIPASAQDSTRLEHSLA
                     TLAEQLNQQLRERDGESKTILSAHLSLIQDDEFAGNIRRLMAEQHQGLGAAIISNMEQ
                     VCAKLSASASDYLRERVSDIRDISEQLLHITWPELKPRNNLVLEKPTILVAEDLTPSQ
                     FLSLDLKNLAGMILEKTGRTSHTLILARASAIPVLSGLPLDAIARYAGQPAVLDAQCG
                     VLAINPNDAVSGYYQVAQTLADKRQKQQAQAAAQLAYSRDNKRIDIAANIGTALEAPG
                     AFANGAEGVGLFRTEMLYMDRDSAPDEQEQFEAYQQVLLAAGDKPIIFRTMDIGGDKS
                     IPYLNIPQEENPFLGYRAVRIYPEFAGLFRTQLRAILRAACFGNAQLMIPMVHSLDQI
                     LWVKGEIQKAIVELKRDCLRHAETITLGIMVEVPSVCYIIDHFCDEVDFFSIGSNDMT
                     QYLYAVDRNNPRVSPLYNPITPSFLRMLQQIVTTAHQRGKWVGICGELGGESRYLPLL
                     LGLGLDELSMSSPRIPAVKSQLRQLDSEACRELARQACECRSAQEIEALLTAFTPEED
                     VRPLLALENIFVDQAFSNKEQAIQFLCGNLGVNGRTEHPFELEEDVWQREEIVTTGVG
                     FGVAIPHTKSQWIRHSSISIARLVKPVDWQSEMGEVELVIMLTLGANEGMNHVKVFSQ
                     LARKLVNKNFRQSLFAAQDAQSILTLLETELTF"
     gene            complement(14726..15583)
                     /locus_tag="PN977_002997"
     CDS             complement(14726..15583)
                     /locus_tag="PN977_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDI6043011.1"
                     /translation="MKAPGLPADQQFFADLFSGLVLNPQLLGRVWFASQPASLPVGSL
                     CIDFPRLDIVLRGEYGNLLEAKQQRMVEGEMLFIPARAANLPVNNKPVMLLSLVFAPT
                     WLGLSFYDSRTTSLLHPARQIQLPSLQRGEGEAMLTALTHLSRSPLEQNIIQPLVLSL
                     LHLCRNVVNMPPGNSQPRGDFLYHSICNWVQDNYAQQLTRESVAQFFNITPNHLSKLF
                     AQHGTMRFIEYVRWVRMAKARMILQKYHLSIHEVAQRCGFPDSDYFCRIFRRQFGLTP
                     GEYSARFQG"
     gene            complement(15596..16330)
                     /gene="ypdB"
                     /locus_tag="PN977_002998"
     CDS             complement(15596..16330)
                     /gene="ypdB"
                     /locus_tag="PN977_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator YpdB"
                     /protein_id="HDI6043012.1"
                     /translation="MKVIIVEDEFLAQQELSWLIKEHSQMEIVGTFDDGLDVLKFLQH
                     NRVDAIFLDINIPSLDGVLLAQNISQFAHKPFIVFITAWKEHAVEAFELEAFDYILKP
                     YQESRITGMLQKLEAAWQQQQTSSTPATTVTRENDTINLVKDERIIVTPINDIYYAEA
                     HEKMTFVYTRRESYVMPMNITEFCSKLPPSHFFRCHRSFCVNLNKIREIEPWFNNTYI
                     LRLKDLDFEVPVSRSKVKEFRQLMHL"
     gene            complement(16345..18042)
                     /gene="ypdA"
                     /locus_tag="PN977_002999"
     CDS             complement(16345..18042)
                     /gene="ypdA"
                     /locus_tag="PN977_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     YpdA"
                     /protein_id="HDI6043013.1"
                     /translation="MHEIFNMLLAVFDRAALMLICLFFLIRIRLFRELLHKSAHSPKE
                     LLAVTAIFSLFALFSTWSGVPVEGSLVNVRIIAVMSGGILFGPWVGIITGVIAGIHRY
                     LIDIGGVTAIPCFITSILAGCISGWINLKIPKAQRWRVGILGGMLCETLTMILVIVWA
                     PTTALGIDIVSKIGIPMILGSVCIGFIVLLVQSVEGEKEASAARQAKLALDIANKTLP
                     LFRHVNSESLRKVCEIIRDDIHADAVAITNTDHVLAYVGVGEHNYQNGDDFISPTTRQ
                     AMNYGKIIIKNNDEAHRTPEIHSMLVIPLWEKGVVTGTLKIYYCHAHQITSSLQEMAV
                     GLSQIISTQLEVSRAEQLREMANKAELRALQSKINPHFLFNALNAISSSIRLNPDTAR
                     QLIFNLSRYLRYNIELKDDEQIDIKKELYQIKDYIAIEQARFGDKLTVIYDIDEEVNC
                     CIPSLLIQPLVENAIVHGIQPCKGKGVVTISVAECGNRVRIAVRDTGHGIDPKVIERV
                     EANEMPGNKIGLLNVHHRVKLLYGEGLHIRRLEPGTEIAFYIPNQRTPVASQATLLL"
     gene            18419..19657
                     /gene="alaC"
                     /locus_tag="PN977_003000"
     CDS             18419..19657
                     /gene="alaC"
                     /locus_tag="PN977_003000"
                     /EC_number="2.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase"
                     /protein_id="HDI6043014.1"
                     /translation="MADTRPERRFTRIDRLPPYVFNITAELKMAARRRGEDIIDFSMG
                     NPDGATPPHIVEKLCTVAQRPDTHGYSTSRGIPRLRRAISRWYQDRYGVEIDPESEAI
                     VTIGSKEGLAHLMLATLDHGDTVLVPNPSYPIHIYGAVIAGAQVRSVPLVEGVDFFNE
                     LERAIRESYPKPKMMILGFPSNPTAQCVELEFFEKVVALAKRYDVLVVHDLAYADIVY
                     DGWKAPSIMQVPGARDVAVEFFTLSKSYNMAGWRIGFMVGNKTLVSALARIKSYHDYG
                     TFTPLQVAAIAALEGDQQCVRDIAEQYKRRRDVLVKGLHEAGWMVEMPKASMYVWAKI
                     PEPYAAMGSLEFAKKLLNEAKVCVSPGIGFGDYGDTHVRFALIENRDRIRQAIRGIKA
                     MFRADGLLPASSKHIHENAE"
     gene            complement(19722..19793)
                     /gene="ypdK"
                     /locus_tag="PN977_003001"
     CDS             complement(19722..19793)
                     /gene="ypdK"
                     /locus_tag="PN977_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_077777854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YpdK"
                     /protein_id="HDI6043015.1"
                     /translation="MKYFFMGISFMVIVWAGTFALMI"
     gene            complement(20149..21069)
                     /gene="lpxP"
                     /locus_tag="PN977_003002"
     CDS             complement(20149..21069)
                     /gene="lpxP"
                     /locus_tag="PN977_003002"
                     /EC_number="2.3.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311285.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) palmitoleoyltransferase"
                     /protein_id="HDI6043016.1"
                     /translation="MFPQCKFSREFLHPRYWLTWFGLGVLWLWVQLPYPVLCFLGTRI
                     GAMARPFLKRRESIARKNLELCFPQHSAEEREKMIAENFRSLGMALVETGMAWFWPDS
                     RVRKWFDVEGLDNLKRAQMQNRGVMVVGVHFMSLELGGRVMGLCQPMMATYRPHNNQL
                     MEWVQTRGRMRSNKAMIGRNNLRGIVGALKKGEAVWFAPDQDYGRKGSSFAPFFAVEN
                     VATTNGTYVLSRLSGAAMLTVTMVRKADYSGYRLYITPEMEGYPADENQAAAYMNKII
                     EKEIMRAPEQYLWIHRRFKTRPVGESSLYI"
     gene            21422..21664
                     /locus_tag="PN977_003003"
     CDS             21422..21664
                     /locus_tag="PN977_003003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdY family protein"
                     /protein_id="HDI6043017.1"
                     /translation="MIYLWMFLALCIVCVSGYIGQVLNVVSAVSSFFGMVILAALIYY
                     FTMWLTGGNELVTGIFMFLAPACGLMIRFMVGYGRR"
     gene            complement(21741..22016)
                     /locus_tag="PN977_003004"
     CDS             complement(21741..22016)
                     /locus_tag="PN977_003004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6043018.1"
                     /translation="MKVNLMLFSLFLLVSIMACNVFAFSISGGGSEASYKETEKTSMM
                     TTTHSTKFQPSQAILLKMREDAPPLNLTDEIPPPFPTKANYLIHPVR"
     gene            22312..22935
                     /locus_tag="PN977_003005"
     CDS             22312..22935
                     /locus_tag="PN977_003005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdX family protein"
                     /protein_id="HDI6043019.1"
                     /translation="MKRLIMATMVTAVLASSTVWAADNAPVAAQQQTQQVQQTAAAER
                     ISEQGLYAMRDVQVARLALFHGDPEKAKELTNEASALLSDDSTEWAKFAKPGKKTNVN
                     DDQYIVINASVGISESYVATPEKEAAIKIANEKMAKGDKKGAMEELRLAGVGVMENQY
                     LMPLKQTRNALADAQKLLDKKQYYEANLALKGAEDGIIVDSEALFVN"
     gene            23447..24697
                     /gene="frc"
                     /locus_tag="PN977_003006"
     CDS             23447..24697
                     /gene="frc"
                     /locus_tag="PN977_003006"
                     /EC_number="2.8.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formyl-CoA transferase"
                     /protein_id="HDI6043020.1"
                     /translation="MSTPLQGIKVLDFTGVQSGPSCTQMLAWFGADVIKIERPGVGDV
                     TRHQLRDIPDIDALYFTMLNSNKRSIELNTKTAEGKEVMEKLIREADILVENFHPGAI
                     DHMGFTWEHIQEINPRLIFGSIKGFDECSPYVNVKAYENVAQAAGGAASTTGFWDGPP
                     LVSAAALGDSNTGMHLLIGLLAALLHREKTGRGQRVTMSMQDAVLNLCRVKLRDQQRL
                     DKLGYLEEYPQYPNGTFGDAVPRGGNAGGGGQPGWILKCKGWETDPNAYIYFTIQEQN
                     WENTCKAIGKPEWITDPAYSTAHARQPHIFDIFAEIEKYTVTIDKHEAVAYLTQFDIP
                     CAPVLSMKEISLDPSLRQSGSVVEVEQPLRGKYLTVGCPMKFSAFTPDIKAAPLLGEH
                     TAAVLQELGYSDDEIAAMKQNHAI"
     gene            24751..>24908
                     /locus_tag="PN977_003007"
     CDS             24751..>24908
                     /locus_tag="PN977_003007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6043021.1"
                     /translation="MSDQLQMTDGMHIIVEALKQNNIDTIYGVVGIPVTDMARHAQAE
                     GIRYIGFR"
BASE COUNT         6141 a         6031 c         6496 g         6240 t
ORIGIN      
        1 tttgtacgta aacgcatcgt ggggctatag ctcagctggg agagcgcttg catggcatgc
       61 aagaggtcag cggttcgatc ccgcttagct ccaccaaatt tcaaaccctc gctgtaaagc
      121 gggggttttt tgtctctgct ttttgccgct tttgtaatac agtctacgtc cgggttagcg
      181 gcgcctggtg aaagcatcat tggatgaaaa atcggcaaca ggctgacccc tgtttgcttc
      241 gcgatgcgaa taaacttatt atttgtgtgc ctgaaaaccc cgatcagtga gagtagtgta
      301 ctcatgtttg tggagcataa cctgataaaa aatatcaaga tattcacact agcgtttacg
      361 ctcaccgtgg tacttattca gctatcccgt tttatttcgc cacttgccat tatccattcc
      421 agttatatct ttctggcgtg gatgccactg tgcgtaatgc tgtcaatctt gtttatcttt
      481 ggctggcgcg gtgtcgttcc cgttttatgc gggatgtttt gcaccaatct gtggaacttt
      541 catctctctt ttttacagac cgcggtcatg ctcggcagcc agacgtttgt tgtgttgtgt
      601 gcctgcgcaa tattacgctg gcagctgggg acgcgttggc gttatggatt gaccagccga
      661 tatgtctggc aacgtctgtt ctggcttggt ttggtggcgc cgattggcat caaatgcagc
      721 atgtatcttg tgggaaattt ctttgatttt ccgctaaaga tatctacctt tttcggcgat
      781 gcggatgcca ttttcacggt cgttgatttg ctaagccttt tcaccgcagt gctgatttac
      841 aacatgcttt tttactatct cacccgcatg attgtaagtc cccactttgc acagatattg
      901 tggcgtaggg atatcgctcc gtcgttgagc aaagagaaac gcgcatttac cttaagctgg
      961 ctggcagctc ttagcgtgct gctacttctg atgtgcacac cgtatgaaaa tgactttatt
     1021 gccggttacc tggtacctgt tttctttatc atctttaccc tcggtgtcgg taagcttcgc
     1081 tatccgtttt taaatctcac ctgggctgtt tcaacgcttt gccttctgaa ttacaaccag
     1141 aactttttgc aaggggtact aaccgaatat tcgctggcat ttattctcgc agtactgatt
     1201 tcctttagtg tttgcctgct ctatatggtg cgtatttatc atcgtagtga atggcttaac
     1261 cgccgctggc atttgcaggc gctgaccgat ccgttaacgc ttctacccaa ctttcgtgcg
     1321 ttggagcaag cgccggagca agaggcgggc aagagttttt gctgcctgcg cattgataat
     1381 cttgagttta tgagtcgtca ttacggctta atgatgcgcg ttcactgtat ccgctcaatt
     1441 taccgtacgc tgctgccgtt gatgcaggaa aacgaaaagt tgtatcaatt gccgggtagt
     1501 gaactgctga tagtgctgag cgggccggaa acggaagggc gactccagca tatggttaac
     1561 atcctgaata gtcggcaaat tcactggaac aataccgggc tggatatggg ctatggtgct
     1621 gcctgggggc gttttgatgg aaatcaggaa accctgcaac ccttgttggg gcagttaagc
     1681 tggctggcgg aacaatcctg cgctcatcat catgtgctgg cgctggatag cagagaggag
     1741 atggtttccg ggcagaccac taaacaggtg ctattgctga ataccattcg tactgcatta
     1801 gatcagggcg atttgctgct ctacgcccag ccaattcgca acaaagaggg tgaaggttat
     1861 gatgagatcc tcgcgcgact gaaatatgac ggcggcatta tgaccccgga taagtttctg
     1921 ccccttattg ctcagtttaa ccttagcgcg cgttttgatt tgcaagtgct ggaatccttg
     1981 ttgaagtggc tggcaacaca cccttgcgac aaaaaaggtc cgcgcttttc agtcaattta
     2041 atgccgctca cgctactgca aaaaaatatc gccgggcgga ttattcgtct gtttaagcgt
     2101 tatcacatct ccccgcaggc ggtcattctt gagatcaccg aggagcaggc gttttctaac
     2161 gcagaaagca gcatgtacaa catcgagcaa ctgcataagt ttggtttccg gattgcgatt
     2221 gatgactttg gcaccggcta tgccaactac gaacggttaa agcgtttgca ggctgatatc
     2281 atcaaaattg atggtgtctt tgtgaaagat atcgtcacga atacgctgga tgcgatgatt
     2341 gtgagatcaa ttaccgatct ggcgaaagcg aagtcattga gtgtggtcgc ggagtttgtc
     2401 gagacgcaac agcagcaggc gctattgcat aagctcgggg tgcaatatct gcaagggtat
     2461 ttgattggtc gcccgcagcc attagctgat taacaggcgt aaaaaaccgg ggaattatcc
     2521 ccggcttttt tatgtatggt cttacagcac cagtgctgcg attgacgcag acagcacact
     2581 caccagggta gagccgtaaa ccagtttcag accgaagcga gaaaccacgt taccttgctc
     2641 ttcattcagg cctttaactg cacctgcgat aatcccgatt gatgagaagt tagcgaagga
     2701 aaccaggaac acagagatga tgccttcagc acgcggagag agcgtggaag caattttctg
     2761 cagatccatc atcgcaacga actcgttgga aaccagtttg gtcgccatga tactgcccac
     2821 ttgcagtgct tcactggaag gaacacccat cacccatgca atcggataga agatgtagcc
     2881 caggatgccc tggaaggaga tgctgtagcc aaaccagcca gtcacggtag caaacagtgc
     2941 gttcagcgcg gcgatcaggg cgataaagcc gatcagcatt gcggcaacga taatggcaac
     3001 tttgaaacct gccagaatgt attcacccag catttcgaag aagctctgac cttcgtgcag
     3061 gttggacatc tgaatgtttt cttcactggc atcaacacgg taagggttga tcagcgacag
     3121 cacgataaag gtgctgaaca tgttcagtac cagcgccgca acgacatatt tcggctccag
     3181 catggtcatg tatgcaccaa cgatggacat cgacacggtg gacatcgccg ttgctgccat
     3241 ggtgtacata cgattgcggg agattttgcc gaggatatct ttataggcaa taaagttttc
     3301 agactgaccc agaatcaggg agctgacggc gttaaaggat tccagtttgc ccataccgtt
     3361 gactttggaa agcaggaaac caattgcgcg gataatcact ggcaacacgc gaatgtgctg
     3421 gagaataccg atcaatgcag agataaagac gattgggcac agcactttca gaaagaagaa
     3481 tgccaggcct tgatcattca tgctaccaaa gacgaagtta gtcccttcgt tggcaaatcc
     3541 gagcagtttt tcgaacattt cggagaagcc tttcacgaag cctaaaccaa cgtcggagtt
     3601 caggaagaac cacgccagta acacttcgat aacaagcagt tgaataacat aacggatacg
     3661 aatttttttg cggtcgctgc ttaccagcag tgcgagaatc gcaacaacgg caagtgccag
     3721 tacaaaatga aggacgcggt ccatatttgc tccaaatatg aggcaggtta aatttccgtg
     3781 cacattctat gtaacatggt taaggaaaac gagatctgac acacactata atgacgtcct
     3841 gtgacaagct tcaaaaatag tgagctgtca tacatttctg ctatgttgtg tatggaagct
     3901 gaaagttatg taaatgtgct aacattaaac ataatcacaa cattcaagtc atcaatcttt
     3961 tcgtcgtcat aattctggtc tatcaaagaa atcatcacaa tccacttaaa tgaatttgat
     4021 aatcattctc gtttgacata gcatgaaaca tagcaaaggc tatgttttag aggcacaaga
     4081 tgactaacta tcgcgttgag agtagcagcg gacgggcggc gcgcaagatg aggctcgcat
     4141 taatgggacc tgcgttcatt gcggcgattg gttatatcga tcctggtaac tttgcgacca
     4201 atattcaggc cggggccagc ttcggctatc agctactgtg ggttgtcgtt tgggccaacc
     4261 tgatggcgat gctgattcag atactgtctg ccaaactggg gattgccacc ggtaaaaacc
     4321 tggcggagca gattcgcgat cactatccgc gtcccgtagt gtggttctat tgggttcagg
     4381 cagaaatcat tgcgatggca accgacctgg cagaatttat tggtgcggcg atcggtttta
     4441 aactcattct gggtgtttcg ctgttgcagg gcgcggtgct gacggggatc gcgactttcc
     4501 tgattttaat gctgcaacgt cgagggcaaa aaccgctgga gaaagtaatt ggcgggttac
     4561 tgctgtttgt tgctgcggct tacattgtcg agttgatttt ctcccaacct aacctggcgc
     4621 aactgggtaa aggaatggta atcccgagtt tacccacctc ggaggcagtc ttcctggcgg
     4681 caggtgtgtt gggggcgacg attatgccgc acgtgattta tttgcactct tcgctgactc
     4741 agcatttaca tggtggttcg cgtcaacaac gttattccgc caccaaatgg gatgtggcta
     4801 tcgccatgac gattgccggt tttgtcaatc tggcgatgat ggctacggct gcggcggcgt
     4861 tccacttttc cggtcatact ggcgttgccg atcttgatga ggcttatctg acgctgcaac
     4921 cgttgttaag ccatgctgcg gcaacggttt ttggtttaag tctggtggct gcggggcttt
     4981 cgtcgacggt ggtggggaca ctggcggggc aggtagtgat gcagggcttc attcgttttc
     5041 atatcccgct gtgggtgcgt cgcactgtta ccatgttgcc gtcatttatt gtcattctga
     5101 tgggattaga tccgacacgg attctggtta tgagtcaggt gctgttaagt tttggtatcg
     5161 ctctggcgct ggttccacta ctaattttta ccagtgacag taagttgatg ggcgatctgg
     5221 tgaacagcaa acgcgtaaaa cagacaggct gggtgattgt ggtgctggta gtggcgctga
     5281 atatttggtt gttagtggga acggcactgg gattgtagtg gaactggtgt cggagcaggc
     5341 atcactggcg gatgcggcgt aaaacgcctt atccgcccta cgtgtttaat ggcgatgtcc
     5401 atgtccgcgg ccttttccgc gatgatcatc acgatcgcgc cagccttcac gatagccacg
     5461 ctcatacgct ttacgcttat cccagccacg gtggtagcca ttatcatgac gccaccagcg
     5521 gttcttacgc cattcataat tgcgatgcca gtagtcacgg tcgcgccagt gaccaccgtc
     5581 ccagtaatta ccgtaatgat cgcgatcgcc aatttgtaat ttgattgatg gcagtagggt
     5641 gatttcacct gcgtttgccg caagcggggt aaatgccatc agggcggccg ccagaaacag
     5701 tgacctgaac attgttattc tccttcacga tcgaagccgt cagcggcctg ttaacgcaat
     5761 attacgggga ggtaaagccc cgtatcatcg ccataactct taaatcataa aggagagcat
     5821 tttttgctaa acgttatcgt ggggtggctg ttgcttcgga cgattgacca tcatcatcgg
     5881 cttacctgct aataacagcc atgccggaag catgacgata cagagcagcg gtagcaatgt
     5941 ggtattgggt acaacgactg ccgccataaa aagacttaac cagccatcgc gtgttaccac
     6001 cagcacgatg ccgagaatag cgcaggaaac ggttattgcc gctggaacgg cgggaacgtg
     6061 ctcatgcagc atcaacccta atgccacccc gacaaacact gccgggaata ttcgcccacc
     6121 gcgaaagccg ctggcggcag caaccacgag agcggcaagt ttaatcacgg ccagcaaaaa
     6181 gtaatcgctg gtgctgaaag cctgatttgc caccatctgc tgcatctcat ccagcccttt
     6241 aaatagcgaa actggtccag caataacccc cagaataccg agaataaatc cgcccactcc
     6301 cagcattagc accggatttt taatctgatg catcatcgcg tgcaaccgtg gcagacacca
     6361 tacggcaacc atccctgctg cgatggcaat cgccgccaca attgcaccgc tgagaatatc
     6421 ggtcatctcc atctgcccat aatgcgcaat gggcagtgaa aaatgaggat ggaaaaacaa
     6481 tccggtggtt agcgcaccag ccgccgccgc cattaacggc gcaaagagac gatcccatag
     6541 cgggacttca ctactgccat ttaaggtttg cgaaaatatc aacgccgccg caacaggtgt
     6601 gccaaacagc gcgccgatgg ttccggcaga ggctaaaata gtccactcca tccgattgac
     6661 gcgcggtaac agacgagcac ctatcgcaac cgcaagggcg atattgacgg tcatgatcgg
     6721 atgttccggc cccaggctga cgccgccagc aagaccgaga attaatgcta cgataagtcc
     6781 tggtagcgca gagggcggaa ccggcgcgcc gatcagcggt tcacaggcgg ggtctggtcc
     6841 agcatgcccc tggctgaaac ggataaccaa ccccaccgca atacctgtta gcgttaatat
     6901 ggcgatgatc cagaaaggtg aatcctgggc tatccccaga gttcccggca gtcgtagcca
     6961 gagcaaattc tgtaataccg aggcgatttt catcaccaca atcagaataa gactggacgc
     7021 aatcccaatt gccacggcgg ggagcgataa taacaacatg gttctggctc gcggatggag
     7081 catgttctat tccttatgcg gggtcagata cttagtttgc ccagcttgca aaaaggcatc
     7141 gctgcaattg gtgctgaaac gataaagtaa ttgtgtgacc cagatcgata tttacaggga
     7201 gcctgccttt ccggcgttgt tgttatgccc ccaggtattt acagtgtgag aaagaattat
     7261 tttgacttta gcggagcagt tgaagaatga caaagtatgc attagtcggt gatgtgggcg
     7321 gcaccaacgc acgtcttgct ctgtgtgata ttgccagtgg tgaaatctcg caggctaaga
     7381 cctattcagg gcttgattac cccagcctcg aagcggtcat tcgcgtttat cttgaagaac
     7441 ataaggtcga ggtgaaagac ggctgtattg ccatcgcttg cccaattacc ggtgactggg
     7501 tggcaatgac caaccatacc tgggcgttct caattgccga aatgaaaaag aatctcggtt
     7561 ttagctatct ggaaattatt aacgatttta ctgctgtatc gatggcgatc ccgatgctga
     7621 aaaaagagca tctgattcag tttggtggcg cagaaccggt agaaggtaag cctattgcgg
     7681 tttacggtgc cggaacgggg ctaggggttg cgcatctggt ccatgtcgat aagcgttggg
     7741 taagcttgcc aggcgaaggc ggtcacgttg attttgcgcc gaatagtgaa gaagagggca
     7801 ttatcctcga aatactgcgt gcagaaattg gtcatgtttc ggcagagcgc gtgctttctg
     7861 gccctgggct ggtgaatttg tatcgcgcaa ttgttaaagc cgacaaccgc ctgccagaaa
     7921 atctcaagcc aaaagatatt accgaacgcg cgctggctga cagctgcacc gattgccgcc
     7981 gcgcgttgtc gctgttttgc gtcattatgg gccgttttgg cggcaatctg gcgctcaatc
     8041 tcgggacatt tggcggcgtg tttattgccg gtggtatcgt gccgcgcttc cttgagttct
     8101 tcaaagcctc tggtttccgt gccgcatttg aagataaagg gcgctttaaa gaatatgtcc
     8161 atgatattcc ggtgtatctc atcgtccatg acaatccggg ccttctcggt tccggcgcac
     8221 atttacgcca gaccttaggc cacattctgt aaatccttcc tgtcaaatcg ggagataact
     8281 ctcccgataa tcttttaaat catacagtta attcaatttt tctttgtgtc acctcacaag
     8341 gccgacctgc gtcacacttc cgtacagcgg gattaattct ccagtaaatg cattatttgt
     8401 ctggtaacgg cgatttgttt tgcacgttca taatttcact cgtcaactat cactgaacga
     8461 ggaattaacg atgagtaaga aactgattgc tttatgtgcc tgcccgatgg gtctggctca
     8521 cacctttatg gcagcccagg cgctggaaga agcggcggtg gaagcaggct atgaagtgaa
     8581 aattgaaact cagggcgcgg acggcatcca gaatcgcctg accgcgcagg atatcgccga
     8641 agcgaccatc atcatccact ccgtggcggt taccccggaa gataacgaac gtttcgaatc
     8701 acgcgacgtt tatgaaatca ctttgcagga cgcaattaaa aacgctgcgg gcatcatcaa
     8761 agaaatcgaa gagatgattg cctctgaaca acagtaatat tcgacaggga taattgttat
     8821 ggccattaaa aaacgcagtg caaccgttgt gcctggcgca tccggtgcgg cagcggcagt
     8881 taagaatccg caggcctcta aaaccagctt ctggggtgaa ctcccgcagc atgtgatgtc
     8941 agggatttca cgcatggtgc cgaccttaat tatgggcggt gtgatcctcg ctttcagcca
     9001 gctgattgct tatagctggc ttaaaattcc tgctgatatc ggcatcatgg atgctcttaa
     9061 tagcgggaaa ttctccggtt tcgacctctc cttactgaaa tttgcctggt tgtcgcagtc
     9121 cttcggcggc gtgttgtttg gttttgccat tccgatgttt gctgcatttg tggcgaactc
     9181 tatcggtggc aaactggcgt tcccggctgg ttttatcggt gggttgatgt ctacccagcc
     9241 gacgcaactg ctgaacttcg atcccagcac tatgcaatgg gcgacctctt cgccggtacc
     9301 gtccaccttc attggtgccc tgattatttc tatcgttgct ggttaccttg tgaagtggat
     9361 gaaccagaaa atccagttac cggatttcct gttagcgttc aaaaccacat ttttgctacc
     9421 gattctttcc gccatttttg tcatgctggc gatgtactac gtcatcaccc cctttggtgg
     9481 ctggatcaac ggcggtatcc gtaccgtgct gactgccgca ggtgagaagg gcgcgctgat
     9541 gtacgcgatg gggatcgccg cggcgacagc aatcgacctt ggtggcccga tcaacaaagc
     9601 agctggtttc gttgccttca gctttaccac tgaccacgta ttaccggtta ccgcgcgttc
     9661 catcgctatt gttattccgc cgattggtct gggtctggcg accattatcg accgccgttt
     9721 aaccggcaaa cgtctgttca gtgctcagct ttatccgcag ggtaaaaccg ccatgttcct
     9781 ggcgtttatg gggatcagtg aaggtgcgat tccgtttgca ctggaaagtc ccatcaccgc
     9841 cattccgtcc tatatggttg gcgcgattgt cggctcaacc gctgcagtct ggctgggcgc
     9901 agtgcaatgg ttcccggagt ccgctatctg ggcatggccg ctggtcacta acctcggcgt
     9961 ctatatggcg gggatcgcgc tgggagcagt cattactgcg ctgatggtgg tgttcctgcg
    10021 tctgatgatg ttccgcaaag gcaaattgtt aatcgatagc ctgtaagaag gacaatcctg
    10081 atgacattac tcgcttcgct gcgcgactgg cttaaggcgc aacaactgga tgcggtgctt
    10141 ctctcctcac ggcagaacaa acagccgcat ctggggatct ccaccggatc aggctatgtg
    10201 ctgattagcc gtgaaagtgc gcacattctg gtggactcgc gctattacgc ggatgtagaa
    10261 gcccgcacgc aaggctacca gctgcatttg cttgacgcga cgcacacgct tgcaaccatc
    10321 gccaggcaaa tcattgccga tgagcagtta aaaacgctcg gttttgaagg ccagcaggtg
    10381 agttgggaaa ccgcgcaccg ctggcagtct gaactcaatg cgaaactggt aagcgccacg
    10441 ccggatgtgc tgcggcaaat caaaacgcca gaggaggtgg agaaaatccg cctcgcctgt
    10501 gggattgccg atcgcggtgc agagcatatt cgccgcttta ttcaggcggg aatgagcgag
    10561 cgcgagatag ccgctgaact ggagtggttt atgcgccagc agggcgcaga aaaagcctct
    10621 ttcgatacca ttgtcgccag cggctggcgt ggggcgctgc cgcacggcaa agccagcgac
    10681 aagattgttg cagcgggcga gtttgtcact cttgattttg gtgcgctgta tcagggctac
    10741 tgctctgata tgacgcgcac cttgctggtg aatggcgaag gggtgagcgc cgaatctcac
    10801 ccgctgtttg acgtctatca gattgttttg caggcacagc tcgcggcaat ctccgcgatt
    10861 cgccccggcg tgcgctgcca gcaggttgac gacgccgcgc gccgggtgat taccgaggct
    10921 ggatttggcg actatttcgg tcataacacc ggtcatgcta tcggcattga agtccatgaa
    10981 ggtccgcgtt tttcaccgcg ggacaccacg acgctacagc caggcatgtt actgaccgtg
    11041 gagccgggga tttatttgcc agggcaaggg ggcgtgcgca tcgaagatgt tgtgctggtc
    11101 accccgcaag gcgcagaagt gctctacgcc atgccgaaaa cagtgttgct cacgggagag
    11161 gcataatgga tttatcgcta ttaaaagcgt tgagcgaggc agatgcgatc gcctcctcgg
    11221 aacaggaagt acggcagatc ctgctggaag aagcggatcg cctgcaaaaa gaagtgcgat
    11281 ttgacggcct gggatcggtg ctgatccgcc tcaatgaatc gacaggtccg aaggtgatga
    11341 tctgtgcaca tatggatgaa gtgggattta tggtgcgcag catctcccgc gaaggggcga
    11401 ttgatgtgct gccagttggc aacgtgcgca tggctgcccg acagcttcag ccggtgcgca
    11461 tcaccacccg tgaagagtgc aaaatacctg gcctgcttga cggcgaccgg caggggaatg
    11521 acgtcagcac catgcgcgtg gacattggcg cgcgctcgtg tgacgaagtg atgcaggcgg
    11581 gaattcgtcc aggcgatcgc gtcaccttcg ataccacttt tcaggttctc cctcaccagc
    11641 gggtgatggg gaaagccttt gatgaccgcc tcggttgcta cctgctggtg acgttactac
    11701 gtgagctgca cgacgccgaa ctgcctgcgg aagtatggct ggtggcaagt tccagcgaag
    11761 aggtgggatt acgcggcggg caaactgcca cccgcgcggt gtcgccggat gtcgccattg
    11821 tgcttgatac cgcctgctgg gcgaaaaact ttgattatgg cgcggctaac catcgtcaga
    11881 ttggtaacgg gccgatgctg gtgttaagcg ataaatcgct gattgcgccg ccaaaactta
    11941 ccgcgtggat tgaaaccgtg gcggcagaaa ttggcgtgcc gttacaggca gatatgttca
    12001 gcaacggcgg cacggacggt ggagcggtgc atttaaccgg cactggcgta cccacagtgg
    12061 tgatggggcc agccacccgc cacggacatt gcgcggcatc gattgccgat tgtcgcgaca
    12121 ttttgcagat gcagcaactt ttatctgccc ttattcaacg tcttacgcgt gagacggttg
    12181 ttcaactgac ggatttcaga tgatctcctg attaacccgg agcggttatg ctaacgattc
    12241 aatttctctg tcctctgcct aacggtctgc atgctcgtcc ggcgtgggaa cttaaagaac
    12301 agtgcagcca gtggcaaagc gaaatcacct tcatcaacca tcgccagaac gcaaaggcag
    12361 atgcgaaaag ctcgctggcg ctgattggca ccggcaccct atttaatgac agttgcagcc
    12421 tgaacattag cggcagcgat gaagagcagg cgcggcgcgt gctggaagag tacatccagg
    12481 tgcgctttat cgacagcgac agcgtccagc caacgcaggc agaactgaca gcgcatccgc
    12541 tgccgcgttc attaagccgt ctgaacccgg atttactgta cggcaatgtg ctggcaagcg
    12601 gcgtcggcgt gggtacgctg accctgttac agagcgacag cctcgacagt tatcgggcca
    12661 tcccggccag tgcgcaagac tccacccggc tggagcacag cctggcaacg cttgccgagc
    12721 aactgaacca gcaactgcgc gagcgtgacg gcgaaagcaa aactatcctc agcgcccatt
    12781 tgtcgctgat ccaggatgat gaatttgcag gcaatatccg tcgcctgatg gcagaacagc
    12841 atcaggggct gggggcggcg atcatcagca atatggagca ggtttgcgcc aaactatctg
    12901 cttctgccag cgattatctg cgcgagcggg tcagcgacat tcgcgatatc agcgaacagt
    12961 tactgcatat cacctggccg gaactgaaac cgcgcaacaa tctggtgctg gaaaaaccga
    13021 ctattctggt ggctgaagat ttaaccccaa gccagttttt gagcctcgat ttaaaaaatc
    13081 ttgcgggcat gattctggag aaaaccgggc gcacctcgca taccctgatc ctggcccgcg
    13141 cttcggcgat cccggtactg agcggcctgc cgctggatgc gattgcccgt tatgccgggc
    13201 aacctgcagt gcttgacgcc cagtgcggcg tgctggcgat taacccgaat gacgcggtga
    13261 gcggttatta tcaggtcgcg cagacgctgg cggataaacg ccaaaaacaa caggcgcagg
    13321 cggccgcgca gctggcctat tcccgtgata acaagcgtat tgatattgcg gcgaatatcg
    13381 gcaccgctct ggaagcgcca ggcgcgtttg ccaacggcgc ggaaggtgtc gggctgtttc
    13441 gtaccgaaat gctctatatg gatcgcgaca gcgcgccgga cgagcaggag caatttgaag
    13501 cctaccagca ggtgctactg gcggcgggcg acaagccgat tatcttccgc acgatggaca
    13561 tcggcggcga taaaagcatt ccttacctca acattcccca ggaagagaac ccgttcctcg
    13621 gctatcgcgc ggtacgtatt tacccggaat ttgctggcct gttccgcact caactgcggg
    13681 cgattttgcg tgctgcctgt ttcggcaacg cccagttgat gatcccgatg gttcacagcc
    13741 tcgatcagat cttatgggtg aagggcgaga tccaaaaagc gatcgttgag cttaagcgcg
    13801 attgcctgcg tcatgcagag acgattacgc tgggtatcat ggtggaagtg ccgtcggtgt
    13861 gctacatcat cgatcacttc tgcgatgagg tcgatttctt cagtatcggc tccaacgata
    13921 tgacccagta tctgtatgcg gtcgatcgta ataacccgcg cgtatcgccg ctgtataacc
    13981 cgattacgcc atcgttcctg cgcatgttgc agcagattgt taccactgcg catcagcggg
    14041 gtaaatgggt aggtatttgc ggtgaactgg gcggtgaaag ccgttatctt ccgctactgc
    14101 ttgggctggg cctggacgag ctgagtatga gtagcccgcg cattccggcg gtgaaaagcc
    14161 agcttcgtca actggatagc gaggcgtgtc gggaactggc gcgtcaggca tgtgaatgcc
    14221 gcagtgcgca ggaaattgaa gcgttactca ccgcctttac gccggaagaa gacgttcgcc
    14281 cactgctggc gctggagaat atctttgttg atcaggcttt tagcaataaa gagcaggcga
    14341 tccagttcct gtgcggtaac ctcggcgtta acgggcgcac tgaacatccg ttcgagctgg
    14401 aagaggatgt ctggcagcgg gaagagattg ttaccaccgg cgttggtttt ggcgtagcga
    14461 tcccgcacac caaatctcag tggatccgtc attccagtat cagcattgcc cggctggtga
    14521 aaccggttga ctggcagtca gaaatgggcg aagtcgaact ggtgatcatg ctgacactgg
    14581 gcgctaacga agggatgaat catgtgaaag tcttctcgca gctggcgcgt aaactggtga
    14641 ataaaaactt ccgccagtcg ctgttcgccg cgcaagatgc gcaaagcatc ctgacgctgc
    14701 tggaaacgga attaaccttc tgacgttagc cctgaaaacg ggcgctgtac tctcccggcg
    14761 tcaggccaaa ctgacgccgg aaaatgcgac aaaaatagtc gctgtccgga aaaccgcaac
    14821 gctgtgccac ttcatgaatc gacagatgat atttctgcaa aatcatcctt gcctttgcca
    14881 ttcgtaccca gcgaacatac tcgataaaac gcattgttcc atgctgagca aacagttttg
    14941 acagatgatt gggcgtgata ttaaaaaact gagccacgct ctcgcgggtg agctgctggg
    15001 cataattatc ctgaacccag ttacaaatgc tgtgatagag aaaatcgccg cgcggctgcg
    15061 aattgcccgg tggcatattc accacgttgc ggcaaagatg cagcaaactt aacaccagtg
    15121 gctgaatgat attctgctcc agcggcgaac ggctaagatg ggtgagggcg gtaagcatcg
    15181 cttcaccttc gccgcgttgc aggctgggaa gctggatctg gcgggcagga tgcaacaacg
    15241 acgtggtgcg gctatcgtaa aacgataacc caagccaggt cggtgcgaac accaggctta
    15301 acagcatcac cggtttgttg ttgaccggta aattagccgc gcgtgccgga ataaacagca
    15361 tttctccttc caccatacgt tgctgctttg cttccagcag attgccgtat tcgccgcgta
    15421 gcacgatatc cagacgggga aaatcaatac ataaactgcc cactggcaat gaggcaggct
    15481 ggctggcaaa ccagacgcgc cccagtagtt gcgggttaag caccaggccg ctgaacagat
    15541 cggcgaaaaa ttgttgatcg gcgggcaagc ctggtgcttt catgaaagtt cccgattaaa
    15601 gatgcattaa ctggcggaat tctttaactt tgctgcggct gaccggcact tcaaaatcca
    15661 gatctttcag ccgcagaatg taggtgttat taaaccacgg ttcgatctcg cggattttgt
    15721 tcagattgac acaaaatgag cgatggcagc ggaaaaaatg cgacggcggc agcttgctgc
    15781 aaaactcggt aatgttcatc ggcattacgt aggattcacg ccgcgtatag acaaaggtca
    15841 ttttctcatg cgcttcggcg taatagatat cgttaattgg cgtgacgatg attcgctcat
    15901 ctttcaccag attaatggtg tcattctcac gcgttaccgt cgtggcaggc gtactgctgg
    15961 tctgctgttg ttgccaggct gcttccagtt tttgtagcat cccggtaatg cgtgactcct
    16021 gatacggttt gagaatgtag tcaaacgcct ccagttcaaa cgcttctacc gcatgttctt
    16081 tccatgcggt gataaacaca ataaacggtt tatgggcgaa ctggctgatg ttttgcgcca
    16141 gcaacacgcc atccagcgac ggaatattga tatccagaaa aatggcgtcg acgcggttat
    16201 gctgcaaaaa cttcaacacg tccagaccgt cgtcaaaggt gccgacaatc tccatctggc
    16261 tgtgctcttt aattagccag ctcagttcct gttgtgccag gaattcgtct tcaacaatga
    16321 tgactttcac aatatcactc cggctcaaag caataacgta gcctgtgagg cgactggggt
    16381 gcgttggtta ggaatgtaaa acgcaatttc cgttcctggt tccaggcggc ggatatgcag
    16441 cccctcgcca tacaataact tcacgcgatg atggacattc agcagaccaa ttttattgcc
    16501 cggcatttcg ttcgcttcga cccgctcaat gacttttgga tcgatgccgt gcccggtgtc
    16561 tcgcaccgca atgcgtaccc gattaccgca ctctgcaacg ctgatggtga caacgccttt
    16621 acctttgcaa ggctgaatac cgtggacaat ggcattctcc accaacggct ggatcagcag
    16681 gctgggaatg cagcaattca cctcttcatc aatatcatag ataaccgtca gcttgtcgcc
    16741 aaagcgggcc tgctcaatgg caatatagtc tttaatttga tacagctctt ttttgatatc
    16801 gatttgctca tcgtctttta attcaatgtt atagcgcaga taacgcgaca gattaaagat
    16861 cagctggcga gcggtatccg gattcagacg gattgacgat gaaatagcgt tcagagcgtt
    16921 aaacagaaaa tggggattaa ttttgctttg cagcgcgcga agctctgcct tatttgccat
    16981 ttcacgtaac tgctcggcac gcgaaacctc cagttgcgtg gagatgattt gcgacagacc
    17041 gaccgccatt tcctgtaatg atgacgtaat ctgatgcgcg tggcagtagt aaattttcag
    17101 tgttccggtc acaacgcctt tctcccacaa tgggatcacc agcatggaat gaatctctgg
    17161 tgtgcggtgg gcttcatcat tgtttttaat gatgattttt ccgtaattca tcgcctgtcg
    17221 ggtagtcggg ctaatgaagt catcgccatt ctgatagttg tgttcaccca cgccgacata
    17281 ggccagcaca tgatcagtat tagtaatcgc caccgcatcg gcgtgaatgt catcgcggat
    17341 aatttcgcag accttgcgta atgactcgct attgacatgg cgaaacagcg gcagcgtttt
    17401 gttggcaata tccagcgcca gcttggcctg ccgcgcggcg ctggcctctt tttcgccctc
    17461 aacgctttgc accagcagca caataaagcc gatacagacg ctaccgagga tcattggaat
    17521 cccgatttta gagacgatat cgatccccaa cgcggtagtt ggtgcccaga caatcaccag
    17581 aatcatggtc aacgtttcac ataacatgcc gccgagaata ccgacgcgcc agcgctgtgc
    17641 tttagggatt ttcaggttga tccagcccga tatacaaccc gccagaatgc tggtgataaa
    17701 gcaggggatc gccgtcacgc cgccgatatc aattaaataa cggtgaatac ctgcgatcac
    17761 gccggtaata atgcctaccc acgggccaaa cagaattccg cctgacatca ccgcgataat
    17821 acgtacgttc accagcgagc cttctacggg aacgccggac caggtgctga acagggcgaa
    17881 cagcgagaaa atggcggtaa cggcgagcag ctctttcggt gagtgtgccg acttgtgcaa
    17941 cagttcgcga aacaggcgga tacggatgag aaagaacagg cagataagca ttaacgctgc
    18001 ccgatcgaag accgccagca gcatgttgaa tatttcgtgc acgggtgact ctgaataagg
    18061 attaaacctc tatgataggt aacctgaagg ctgatgacca gcaggccgtt tttgaggaaa
    18121 aaccacaatt ttaaaagggg attgaaagat aaatggtgta ttagcaatgc atctcttgat
    18181 aatgcacatc tggttggtaa atttattgaa attataatgt taataaaatg agtggtaatt
    18241 ttcttgttag cgtagataaa tttcctgttt ttgaagtgga gttcctcatt taaaaccatt
    18301 gataaaaatg ttctaaaacg ctggacagac agaaattaat caggctattt tcaaaacagc
    18361 caccatcgtg gcagcgtcgc tcggacggtc cgggcgctaa cgttaatctg aggaaattat
    18421 ggctgacact cgccctgaac gtcgctttac gcgtattgat cgtctcccgc cctacgtttt
    18481 taatattacc gctgaactga aaatggctgc gcgtcggcgc ggcgaagata ttatcgattt
    18541 cagtatgggt aacccggacg gtgcgactcc gccgcatatc gtcgaaaaat tatgtacggt
    18601 ggcccagcgc ccggacacgc atggttactc cacgtcgcgc ggcattccgc ggttacgtcg
    18661 cgccatttcc cgctggtatc aggatcgcta cggcgttgag atcgacccgg aatcagaagc
    18721 tatcgtcacc attggttcga aagagggact ggcgcatctg atgctggcga cgctggatca
    18781 tggtgacact gtactggtgc ctaatccaag ctacccgatt catatttacg gcgcggtgat
    18841 tgccggggcg caggtacgct cagtgccgct ggtggaaggt gtcgatttct tcaacgaact
    18901 ggaacgcgcc attcgcgaaa gttatccgaa accgaagatg atgatcctcg gcttcccgtc
    18961 gaacccaacc gcgcaatgcg tggagctgga attctttgag aaggttgtgg cgctggcgaa
    19021 acgctacgat gtgctggtgg tccatgacct ggcctatgcc gatatcgtct acgatggctg
    19081 gaaagcgccg tcaatcatgc aggtgccagg tgcacgcgat gtggcggtcg agttctttac
    19141 gctgtcgaaa agttacaaca tggcgggctg gcgtatcggc tttatggtcg gtaacaaaac
    19201 gctggtcagc gcgttggcac gtattaaaag ctatcacgat tacggcacct ttacgccgtt
    19261 gcaggtggca gcgattgcgg cgctggaggg cgatcaacag tgcgtgcgcg acattgctga
    19321 acagtacaaa cgccgccgtg atgtactggt aaaagggctg catgaagcgg gctggatggt
    19381 cgaaatgccg aaggcttcga tgtatgtctg ggcgaaaatc ccggaaccat atgcggccat
    19441 gggttcactg gaatttgcca agaagctgct taacgaagcg aaggtctgtg tctctccagg
    19501 gattggcttt ggcgactacg gcgacaccca tgttcgcttt gcactgattg aaaaccgcga
    19561 tcgtattcgc caggcgattc gtggaattaa agcgatgttc cgtgccgacg gtttactacc
    19621 cgccagcagc aaacatattc acgaaaacgc ggaataaaca ccatcgccag aaatgcaaac
    19681 aggagccaga tggctcctgt ttttcattgc acagtttgct tttagatcat cagggcaaaa
    19741 gtaccggccc aaacgatgac cataaaagaa atgcccataa agaaatattt cactttacat
    19801 ctctccgcgt aattactgtt acgcctgaat gacttcatta cacctgatac agtgagttga
    19861 caccgggcaa aacgacctta aaagtcctgt attttaccgt gggcatcacc ccagaattct
    19921 gagctaaact gctccagacg gcgctaatgt acgcctaaaa ctgaaacgag acaagaggca
    19981 ttttcttatt tacttttagt aacttacagg tgtctcatat cggcgacagt ttaatttctg
    20041 acggtaataa ttagagattc agcgaaggaa aaaaccttaa gaaaaaagcc ataaaaacca
    20101 tgaggttatt atggctgatt tgaggaggga aagagtaaga gcagtttgtt aaatgtatag
    20161 cgacgattct cccaccgggc gcgttttaaa gcgacggtgg atccataggt actgctccgg
    20221 tgcgcgcatg atctcttttt cgataatctt gttcatatag gcagcggctt gattttcatc
    20281 tgccgggtag ccttccatct ctggggtgat gtacaaacga tatccgctgt aatccgcttt
    20341 tcttaccatc gttacggtta acatggctgc gccagagaga cgtgaaagga catacgtacc
    20401 attggttgtg gcaacatttt ccaccgcaaa gaacggcgcg aaggagctgc ctttacgacc
    20461 ataatcctga tcgggagcaa accataccgc ttcacctttc ttcagtgcac cgacaatgcc
    20521 gcgcagatta tttctgccga tcatcgcttt gttagagcgc atgcgaccac gggtctgcac
    20581 ccattccatc agctgattat tatgtggacg ataggtggcc atcattggct gacaaagtcc
    20641 catcacgcgg ccgcccagtt ccagcgacat aaaatggacg ccgacaacca ttacgccgcg
    20701 attttgcatt tgtgcgcgtt taagattatc caacccttca acatcaaacc atttacgtac
    20761 gcgactgtcg ggccagaacc acgccatgcc ggtttctacc agcgccatgc cgagtgaacg
    20821 aaagttttcg gcaatcatct tctcgcgttc ttccgcagaa tgctgcggga aacaaagttc
    20881 caggttttta cgggcgatag attcacgccg tttcaggaat ggtcgcgcca ttgcgccaat
    20941 acgcgtgccg agaaagcaga gaacaggata aggaagttgt acccaaagcc agagtacacc
    21001 aagcccaaac catgtgagcc agtagcgagg atgcagaaac tcgcgggaaa atttgcattg
    21061 tggaaacata gttaccttct gtagatacga attgttgtat cgcagaagat tccatgtcga
    21121 cagcggtaca ctcaattgat taggccatca tgtacgacaa tggttcctgt aaagagtgta
    21181 aagactagtc aggacataac cacgaggagc atgaatgcgg tggctctact ttatcactgg
    21241 ctgactgctt tcagctactg attattaatc gtgcaaagta aaaagttttc cgcacgcaat
    21301 agcgttgcat ttaacgtgct gttttcattg atgcttagca aaaattgcca gaggttaagg
    21361 attgtcttac tattaaagtg actgaagtta caggtttttt agcgcggcag gaggttgagc
    21421 tatgatttat ttatggatgt ttctcgccct gtgtattgtc tgcgtaagcg gatacatcgg
    21481 tcaggtcctg aacgtggtct cggcggtctc ttctttcttc ggcatggtga tcctcgctgc
    21541 actcatttac tacttcacca tgtggttaac tggcggtaat gaactagtga cggggatatt
    21601 tatgtttctt gccccggctt gtggcttgat gattcgcttt atggtggggt atggcaggcg
    21661 ctagtgggtc aggatggttc atttgggcag gtgttcacat cagcatcagg catgattgtt
    21721 cacctgccat gtgacagacg ctatcgcaca ggatggataa gataattcgc ctttgtcgga
    21781 aaagggggcg gaatttcatc tgtgaggttt aatggtggcg catcttctct catttttaac
    21841 aaaatcgcct gtgatggctg aaattttgta gaatgagttg tcgtcatcat tgacgttttt
    21901 tctgtctctt tatagctcgc ctcactacca ccaccagaaa tagaaaatgc aaaaacattg
    21961 catgccataa tagagaccaa taaaaataag ctaaaaagca ttaagtttac tttcatagaa
    22021 agttatcctg aaaatggttc actgaaaacc tacgacgtta ttgctgtatt tacatataat
    22081 taatcagtat ttacatcgat ataataaatg acatctcttt ttggtatata agaatagttc
    22141 tctgtgacag gaagcatatt cctacaactg taagactaaa tgccgctttg cgataataac
    22201 tacagttgta agataaccct ttcaaaatgt ccgttgctct ctgatttctc atttcatgct
    22261 cacccaatat gatggcggcg ttttctaaaa ctgttaaaga atgaggtaag tatgaaacgt
    22321 ttaattatgg ctacgatggt cacagcagtt ctggcatctt caactgtatg ggctgctgat
    22381 aatgcaccgg tagcggcaca acagcaaact cagcaagttc agcaaacggc agcagcagag
    22441 cgtatctctg aacaaggttt atatgcgatg cgtgacgtgc aggtagcgcg cctggctctg
    22501 tttcatggcg atccggaaaa ggcaaaagaa ctgaccaatg aagcttccgc tttgttgtca
    22561 gatgacagta ccgaatgggc aaagttcgca aagccaggta aaaagaccaa tgtcaatgat
    22621 gaccaatata ttgtcattaa tgcatctgtc ggaatttctg agagttatgt agcgacacct
    22681 gaaaaagaag ccgcgataaa aattgccaac gaaaaaatgg cgaaagggga taaaaagggc
    22741 gcaatggaag agcttcgcct ggccggtgtt ggtgtaatgg aaaaccaata tctgatgccg
    22801 cttaaacaga ctcgtaatgc acttgctgat gcgcagaaac tgcttgataa aaagcaatac
    22861 tacgaggcaa acctggcgct taaaggtgcg gaagatggaa tcatcgtcga tagcgaagca
    22921 ctgtttgtga actaaagctg acaggcaata tattgaggta aaggatttta ctccctccag
    22981 ccgacaattt tttatggcca caatacgaca ggttgtggcc ataaaattat ttggcgctcg
    23041 cggttctata aactcgaatt ttatcttcct caaagtaaac cgcaataatc ccgtgttatc
    23101 acaaatcatt tattatgtta tgttttatgg tacgctttaa aaagcgtaat cataccctta
    23161 cttgtaaatc actggcgctt cttacagttg taagaataac atcacaaatg tcgcggctct
    23221 ctctgattac cgcgagccaa tatttatcat attcttcttt tcgtcctcac tacttaccat
    23281 gcagaaagga attggaagta gcgatgaaat gattttcagt acttgataca ggaatttaaa
    23341 aatatcgagt gcagctacga cgatattttg cggattattt atagaggcgt tatgtcttaa
    23401 ataaacgcta aattaacaca tatttaatta actaataggt attgctatgt caactccact
    23461 tcaaggaatt aaagttctcg atttcaccgg tgtgcaatct ggcccatctt gtactcaaat
    23521 gctggcctgg tttggcgctg acgtcattaa aattgaacgt cccggcgttg gtgacgtaac
    23581 gcgtcaccag ctgcgagata ttcctgatat cgatgcgctt tacttcacca tgcttaacag
    23641 taacaaacgt tctattgaat taaataccaa aacagcggaa ggcaaagagg taatggaaaa
    23701 gctgatccgc gaagctgata tcttagtcga gaactttcat cctggggcca ttgatcacat
    23761 gggcttcacc tgggagcata ttcaagaaat caatccacgt ctgatttttg gttcgatcaa
    23821 agggtttgat gagtgttcgc cttatgtgaa tgtaaaagcc tatgaaaacg ttgctcaggc
    23881 agcgggtggc gcggcatcca ctacgggttt ttgggacggt ccgccgctgg taagcgctgc
    23941 agcgttgggt gacagcaaca ccggaatgca tttactgatc ggtttacttg ctgctttgct
    24001 gcatcgcgaa aaaacggggc gtgggcaacg agtcaccatg tcaatgcagg atgccgtatt
    24061 gaacctttgc cgcgtgaaat tacgcgacca gcagcgtctc gataaattgg gttatctgga
    24121 agaatacccg cagtatccga atggcacatt tggtgatgca gttccccgcg gaggtaatgc
    24181 gggtggtggc ggtcagcctg gctggatcct gaaatgtaaa ggctgggaaa ccgatcctaa
    24241 tgcctatatt tatttcacta ttcaggagca aaactgggaa aacacctgta aagctatcgg
    24301 caaaccagaa tggattaccg atccggcata cagtacagcc catgcccgac agccacatat
    24361 tttcgatatt tttgctgaaa tcgaaaaata cactgtcact attgataaac atgaagctgt
    24421 ggcctatttg actcagtttg atattccttg tgcaccggtt ttaagtatga aagaaatttc
    24481 acttgatccc tctttgcgcc aaagtggcag tgttgtcgaa gtggaacaac cgttgcgtgg
    24541 aaaatatctg acagttggtt gtccaatgaa attctctgcc tttacgccgg atattaaagc
    24601 tgcgccgcta ttaggtgaac ataccgctgc tgtattgcag gagctgggtt atagcgacga
    24661 tgaaattgct gcaatgaagc aaaaccacgc catctgatat taacgggggc ttctggctcc
    24721 caatttataa aaatttcgag gttattaatc atgtcagatc aacttcaaat gacagatggt
    24781 atgcatatca tcgttgaagc attaaaacag aataatattg acactattta tggtgttgta
    24841 ggtattcctg tgacggatat ggcacgccat gcccaggcgg aaggcattcg ttatattggt
    24901 tttcgtca
//
LOCUS       DAMZFT010000074        24203 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.074, whole genome shotgun sequence.
ACCESSION   DAMZFT010000074 DAMZFT010000000
VERSION     DAMZFT010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 24203)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24203)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24203
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.074"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..356
                     /locus_tag="PN977_003008"
     CDS             <1..356
                     /locus_tag="PN977_003008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HDI6043022.1"
                     /translation="QARLRVALRRHSATTAPDPLVKFSDVTVDLAARVIHRGEEEVHL
                     TPIEFRLLAVLLNNAGKVLTQRQLLNQVWGPNAVEHSHYLRIYMGHLRQKLEQDPARP
                     RHFITETGIGYRFML"
     gene            complement(572..829)
                     /locus_tag="PN977_003009"
     CDS             complement(572..829)
                     /locus_tag="PN977_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6043023.1"
                     /translation="MQKPLSVRWLIIQGLTENEHCGIQAYACNTESQEDLYAEVPLVG
                     RLTGIEEVKVEAIAMSKLHNMSCPPYMGHSAAVIFHLIYLS"
     gene            952..3150
                     /gene="speF"
                     /locus_tag="PN977_003010"
     CDS             952..3150
                     /gene="speF"
                     /locus_tag="PN977_003010"
                     /EC_number="4.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase SpeF"
                     /protein_id="HDI6043024.1"
                     /translation="MSKLKIAVSDSCPDCFTTQRECIYINENSNIDVAAIVLSLNDVT
                     RGKLDEIDATGYGIPVFIATENQERVPAEYLPRISGVFENCESRREFYGRQLETAASH
                     YETQLRPPFFRALVDYVNQGNSAFNCPGHQGGEFFRRHPAGNQFVEYFGEALFRADLC
                     NADVAMGDLLIHEGAPCIAQQHAAKVFNADKTYFVLNGTSSSNKVVLNALLTPGDLVL
                     FDRNNHKSNHHGALLQAGATPVYLETARNPYGFIGGIDAHCFEENYLRELITEVAPQR
                     AKEARPFRLAVIQLGTYDGTIYNARQVVDKIGHLCDYILFDSAWVGYEQFIPMMADCS
                     PLLLDLNENDPGILVTQSVHKQQAGFSQTSQIHKKDSHIKGQQRYVPHKRMNNAFMMH
                     ASTSPFYPLFAALDINAKMHEGVSGRNMWMDCVVNGINARKLILDNCQHIRPFVPELV
                     DGKPWQSYETAQIAVDLRFFQFVPGEHWHSFEGYAENQYFVDPCKLLLTTPGIDARNG
                     EYEAFGVPATILANFLRENGVVPEKCDLNSILFLLTPAEDMAKLQQLAALLVRFEKLL
                     ESDAPLSEVLPSIYKQHEERYTGYTLRQLCQEMHDLYARHNVKQLQKEMFRKEHFPRV
                     SMNPQEANYAYLRGEVELVRLPDAEGRIAAEGALPYPPGVLCVVPGEIWGGAVLRYFS
                     ALEEGINLLPGFAPELQGVYIEEHDGRKQVWCYVIKPRGAQSTLLKGEKL"
     gene            3147..4466
                     /gene="potE"
                     /locus_tag="PN977_003011"
     CDS             3147..4466
                     /gene="potE"
                     /locus_tag="PN977_003011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine-ornithine antiporter"
                     /protein_id="HDI6043025.1"
                     /translation="MSQAKSNKMGVVQLTILTMVNMMGSGIIMLPTKLAEVGTISIIS
                     WLVTAVGSMALAWAFAKCGMFSRKAGGMGGYAEYAFGKSGNFMANYTYGVSLLIANVA
                     IAISAVGYGTELLGASLSPVQIGLATIGVLWICTVANFGGARITGQISSITVWGVIIP
                     VVGLCIIGWFWFSPTLYANSWNPHHVPFFSAVGSSIAMTLWAFLGLESACANAEVVEN
                     PERNVPIAVLGGTLGAAVIYIVSTNVIAGIVPNMELANSTAPFGLAFAQMFTPEVGKV
                     IMALMVMSCCGSLLGWQFTIAQVFKSSSDEGYFPKIFSRVTKVDAPVQGMLIIVIIQS
                     GLALMTISPSLNSQFNVLVNLAVVTNIIPYILSMAALVIIQQVANVPPSKAKVANFVA
                     FVGAMYSFYALYSSGEEAMLYGSIVTFLGWTLYGLVSPRFELKNKHG"
     gene            4815..5465
                     /locus_tag="PN977_003012"
     CDS             4815..5465
                     /locus_tag="PN977_003012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001208812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2625 domain-containing protein"
                     /protein_id="HDI6043026.1"
                     /translation="MREVSQLVNTEDSAWPIIQNWLKEATNHAELLPVNKDLAETALY
                     QLQVTTKSPMGALVYGSGGLLIDNGWLRIAGSGHPRLPRDPASWTQRPEFASVRALPI
                     ADDVAGGIFALNGGDLGEDTGCVYYFAPDTLNWESLEVGYSEFLQWALSGDLDTFYEN
                     VRWPQWQEDVIKLSATEAFTFYPFLWVQCEEARTRKVISLTELWEMQYQMKETITQ"
     gene            complement(5506..6000)
                     /locus_tag="PN977_003013"
     CDS             complement(5506..6000)
                     /locus_tag="PN977_003013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6043027.1"
                     /translation="MKTKRSLVVRVALITATLLLTACAQPEQSSLAGDWLLTPKDKTR
                     GLTGSITVNIAPSRCKTNCRGDNLPDNTRRWQLSGGNEKELTYLHDMSAQEKTGLNPE
                     WQCYTSFFMGICQGKPGTRPLVNEDYVSESGFFGSMMHVGIIELRRCQSENCQQELKA
                     INTH"
     gene            complement(6205..7845)
                     /gene="pgm"
                     /locus_tag="PN977_003014"
     CDS             complement(6205..7845)
                     /gene="pgm"
                     /locus_tag="PN977_003014"
                     /EC_number="5.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-glucose phosphate-specific
                     phosphoglucomutase"
                     /protein_id="HDI6043028.1"
                     /translation="MAIHNRAGQPAQQSDLINVAQLTAQYYVLKPEAGNAEHAVKFGT
                     SGHRGSAARHSFNEPHILAIAQAIAEERAKNGITGPCYVGKDTHALSEPAFISVLEVL
                     AANGVDVIVQENNGFTPTPAVSNAILVHNKKGGPLADGIVITPSHNPPEDGGIKYNPP
                     NGGPADTNVTKVVEDRANALLADGLKGVKRISLDEAMASGHVKEQDLVQPFVEGLADI
                     VDMAAIQKAGLTLGVDPLGGSGIEYWKRIGEYYNLNLTIVNDQVDQTFRFMHLDKDGA
                     IRMDCSSECAMAGLLALRDKFDLAFANDPDYDRHGIVTPAGLMNPNHYLAVAINYLFQ
                     HRPQWGKDVAVGKTLVSSAMIDRVVNDLGRKLVEVPVGFKWFVDGLFDGSFGFGGEES
                     AGASFLRFDGTPWSTDKDGIIMCLLAAEITAVTGKNPQEHYNELAERFGAPSYNRLQA
                     AATSAQKAALSKLSPEMVSSSTLAGDPITARLTAAPGNGASIGGLKVMTDNGWFAARP
                     SGTEDAYKIYCESFLGEEHRKQIEKEAVEIVSEVLKNA"
     gene            complement(7871..8416)
                     /gene="seqA"
                     /locus_tag="PN977_003015"
     CDS             complement(7871..8416)
                     /gene="seqA"
                     /locus_tag="PN977_003015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation negative regulator SeqA"
                     /protein_id="HDI6043029.1"
                     /translation="MKTIEVDDELYSYIASHTKHIGESASDILRRMLKFSAASQPAAP
                     VTKEVRVASPAIVEAKPVKTIKDKVRAMRELLLSDEYAEQKRAVNRFMLLLSTLYSLD
                     AQAFAEATESLHGRTRVYFAADEQTLLKNGNQTKPKHVPGTPYWVITNTNTGRKCSMI
                     EHIMQSMQFPAELIEKVCGTI"
     gene            8601..9365
                     /gene="ybfF"
                     /locus_tag="PN977_003016"
     CDS             8601..9365
                     /gene="ybfF"
                     /locus_tag="PN977_003016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HDI6043030.1"
                     /translation="MKLNIRAQTAQNQHNNSPIVLVHGLFGSLDNLGVLARDLVNDHN
                     IIQVDMRNHGLSPRDPVMNYPAMAQDLVDTLDAQQIDKATFIGHSMGGKAVMALTALA
                     PDRIDKLVAIDIAPVDYHVRRHDEIFAAINAVSESDAQTRQQAAAIMRQHLNEEGVIQ
                     FLLKSFVDGEWRFNVPVLWDQYPHIVGWEKIPAWDHPTLFIPGGNSPYVSEQYRDDLL
                     AQFPQARAHVIAGAGHWVHAEKPDAVLRAIRRYLND"
     gene            9505..9798
                     /gene="ybfE"
                     /locus_tag="PN977_003017"
     CDS             9505..9798
                     /gene="ybfE"
                     /locus_tag="PN977_003017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA regulated protein"
                     /protein_id="HDI6043031.1"
                     /translation="MAKEQTDRTTLDLFAHERRPGRPKTNPLSRDEQLRINKRNQLKR
                     DKVRGLKRVELKLNAEAVEALNELAESRNMSRSELIEEMLMQQLAALRSQGIV"
     gene            9938..10468
                     /gene="fldA"
                     /locus_tag="PN977_003018"
     CDS             9938..10468
                     /gene="fldA"
                     /locus_tag="PN977_003018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldA"
                     /protein_id="HDI6043032.1"
                     /translation="MAITGIFFGSDTGNTENIAKMIQKQLGKDVADVHDIAKSSKEDL
                     EAYDILLLGIPTWYYGEAQCDWDDFFPTLEEIDFNGKLVALFGCGDQEDYAEYFCDAL
                     GTIRDIIEPRGATIVGHWPTAGYHFEASKGLADDDHFVGLAIDEDRQPELTAERVEKW
                     VKQISEELHLDEILNA"
     gene            10678..10764
                     /gene="uof"
                     /locus_tag="PN977_003019"
     CDS             10678..10764
                     /gene="uof"
                     /locus_tag="PN977_003019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001491546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fur leader peptide"
                     /protein_id="HDI6043033.1"
                     /translation="MIRIISRANSVTSSNEVNRLVTGQIPHD"
     gene            10757..11203
                     /gene="fur"
                     /locus_tag="PN977_003020"
     CDS             10757..11203
                     /gene="fur"
                     /locus_tag="PN977_003020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric iron uptake transcriptional regulator"
                     /protein_id="HDI6043034.1"
                     /translation="MTDNNTALKKAGLKVTLPRLKILEVLQEPDNHHVSAEDLYKRLI
                     DMGEEIGLATVYRVLNQFDDAGIVTRHNFEGGKSVFELTQQHHHDHLICLDCGKVIEF
                     SDDSIEARQREIAAKHGIRLTNHSLYLYGHCAEGDCREDEHAHEGK"
     gene            complement(11287..11613)
                     /locus_tag="PN977_003021"
     CDS             complement(11287..11613)
                     /locus_tag="PN977_003021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6043035.1"
                     /translation="MKKLILIAIMASGLVACVQSTAPQEDSRLKEAYSACINTAQGSP
                     EKIEACQSVLNVLKKEKQHQQFADQESVRVLDYQQCLRATQTGNDQAVKADCDKVWQE
                     IRSNNK"
     gene            complement(11663..13069)
                     /gene="chiP"
                     /locus_tag="PN977_003022"
     CDS             complement(11663..13069)
                     /gene="chiP"
                     /locus_tag="PN977_003022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitoporin"
                     /protein_id="HDI6043036.1"
                     /translation="MRAFSGKRSTLALAIAGVTAMSGFMAIPEARAEGFIDDSTLTGG
                     IYYWQRERDRKDVTDGDKYKTNLSHSTWNANLDFQSGYAADMFGLDIAAFTAIEMAEN
                     GDSSHPNEIAFSKSNKAYDEDWSGDKSGISLYKAAAKFKYGPVWARAGYIQPTGQTLL
                     APHWSFMPGTYQGAEAGANFDYGDAGALSFSYMWTNEYKAPWHLEMDEFYQNDKTTKV
                     DYLHSLGAKYDFKNNFVLEAAFGQAEGYIDQYFAKASYKFDIAGSPLTTSYQFYGTRD
                     KVDDRSVNDLYDGTAWLQALTFGYRAADVVDLRLEGTWVKADGQQGYFLQRMTPTYAS
                     SNGRLDIWWDNRSDFNANGEKAIFFGAMYDLKNWNLPGFAIGASYVYAWDAKPATWQS
                     NPDAYYDKNRTIEESAYSLDAVYTIQDGRAKGTMFKLHFTEYDNHSDIPSWGGGYGNI
                     FQDERDVKFMVIAPFTIF"
     gene            complement(13511..15175)
                     /gene="glnS"
                     /locus_tag="PN977_003023"
     CDS             complement(13511..15175)
                     /gene="glnS"
                     /locus_tag="PN977_003023"
                     /EC_number="6.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase"
                     /protein_id="HDI6043037.1"
                     /translation="MSEAEARPTNFIRQIIDEDLASGKHTTVHTRFPPEPNGYLHIGH
                     AKSICLNFGIAQDYKGQCNLRFDDTNPVKEDIEYVESIKNDVEWLGFHWSGNVRYSSD
                     YFDQLHAYAIELINKGLAYVDELTPEQIREYRGTLTQPGKNSPYRDRSVEENLALFEK
                     MRTGGFEEGKACLRAKIDMASPFIVMRDPVLYRIKFAEHHQTGNKWCIYPMYDFTHCI
                     SDALEGITHSLCTLEFQDNRRLYDWVLDNITIPVHPRQYEFSRLNLEYTVMSKRKLNL
                     LVTDKHVEGWDDPRMPTISGLRRRGYTAASIREFCKRIGVTKQDNTIEMASLESCIRE
                     DLNENAPRAMAVIDPVKLVIENYQGEGEMVTMPNHPNKPEMGSRQVPFSGEIWIDRAD
                     FREEANKQYKRLVLGKEVRLRNAYVIKAERVEKDAEGNITTIFCTYDADTLSKDPADG
                     RKVKGVIHWVSAAHALPVEIRLYDRLFSVPNPGAADDFLSVINPESLVIKQGFAEPSL
                     KDAVAGKAFQFEREGYFCLDSRHSTAEKPVFNRTVGLRDTWAKVGE"
     gene            complement(15378..17324)
                     /gene="nagE"
                     /locus_tag="PN977_003024"
     CDS             complement(15378..17324)
                     /gene="nagE"
                     /locus_tag="PN977_003024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetyl glucosamine transporter subunit
                     IIABC"
                     /protein_id="HDI6043038.1"
                     /translation="MNILGFFQRLGRALQLPIAVLPVAALLLRFGQPDLLNVAFIAQA
                     GGAIFDNLALIFAIGVASSWSKDSAGAAALAGAVGYFVLTKAMVTINPEINMGVLAGI
                     ITGLVGGAAYNRWSDIKLPDFLSFFGGKRFVPIATGFFCLVLAAIFGYVWPPVQHAIH
                     AGGEWIVSAGALGSGIFGFINRLLIPTGLHQVLNTIAWFQIGEFTNAAGTVFHGDINR
                     FYAGDGTAGMFMSGFFPIMMFGLPGAALAMYFAAPKERRPMVGGMLLSVAVTAFLTGV
                     TEPLEFLFMFLAPLLYLLHALLTGISLFVATLLGIHAGFSFSAGAIDYALMYNLPAAS
                     QNVWMLLVMGVVFFAIYFVVFSLVIRMFNLKTPGREDKEDEIVTEEANSNTEEGLNQL
                     ATNYIAAVGGTDNLKAIDACITRLRLTVADSARVNDTMCKRLGASGVVKLNKQTIQVI
                     VGAKAESIGDAMKKVVARGPVAAASAETAPATAAPVAKPQAVPNAVSIAELVSPITGD
                     VVALDQVPDEAFASKAVGDGVAVKPTDKIVVSPAAGTIVKIFNTNHAFCLETEKGAEI
                     VVHMGIDTVALEGKGFKRLVEEGAQVSAGQPILEMDLDYLNENARSMISPVVCSNIDD
                     FSGLIIKAQGHVVAGQTPLYEIKK"
     gene            17657..18457
                     /gene="nagB"
                     /locus_tag="PN977_003025"
     CDS             17657..18457
                     /gene="nagB"
                     /locus_tag="PN977_003025"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008805656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HDI6043039.1"
                     /translation="MRLIPLTTAEQVGKWAARHIVNRINAFKPTADRPFVLGLPTGGT
                     PMTTYKALVEMHKAGQVSFKHVVTFNMDEYVGLPKEHPESYYSFMHRNFFDHVDIPAE
                     NINLLNGNAPDIDAECRQYEEKIRSYGKIHLFMGGVGNDGHIAFNEPASSLASRTRIK
                     TLTHDTRVANSRFFDNDVNQVPKYALTVGVGTLLDAEEVMILVLGSQKALALQAAVEG
                     CVNHMWTISCLQLHPKAIMVCDEPSTMELKVKTLRYFNELEAENIKGL"
     gene            18517..19665
                     /gene="nagA"
                     /locus_tag="PN977_003026"
     CDS             18517..19665
                     /gene="nagA"
                     /locus_tag="PN977_003026"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HDI6043040.1"
                     /translation="MYALTQGRIFTGHEFLDDHAVVIADGLIKSVCPVAELPPEIEQR
                     SLNGAILSPGFIDVQLNGCGGVQFNDTAEAVSVETLEIMQKANEKSGCTNYLPTLITT
                     SDELMKQGVRVMREYLAKHPNQALGLHLEGPWLNLVKKGTHNPNFVRKPDAALVDFLC
                     ENADVITKVTLAPEMVPAEVISKLANTGIVVSAGHSNATLKEAKAGFRAGITFATHLY
                     NAMPYITGREPGLAGAILDEADIYCGIIADGLHVDYANIRNAKRLKGDKLCLVTDATA
                     PAGANIEQFIFAGKTIYYRNGLCVDENGTLSGSSLTMIEGVRNLVEHCGIALDEVLRM
                     ATLYPARAIGVEKRLGTLAAGKVANLTAFTPDFKITRTIVNGNEVVTQ"
     gene            19674..20894
                     /gene="nagC"
                     /locus_tag="PN977_003027"
                     /gene_synonym="nagR"
     CDS             19674..20894
                     /gene="nagC"
                     /locus_tag="PN977_003027"
                     /gene_synonym="nagR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NagC"
                     /protein_id="HDI6043041.1"
                     /translation="MTPGGQAQIGNVDLVKQLNSAAVYRLIDQYGPISRIQIAEQSQL
                     APASVTKITRQLIERGLIKEVDQQASTGGRRAISIVTETRNFHAIGVRLGRHDATITL
                     FDLSSKVLAEEHYPLPERTQQTLEHALLNAIAQFIDSYQRKLRELIAISVILPGLVDP
                     DSGKIHYMPHIQVENWGLVEALEERFKVTCFVGHDIRSLALAEHYFGASQDCEDSILV
                     RVHRGTGAGIISNGRIFIGRNGNVGEIGHIQVEPLGERCHCGNFGCLETIAANAAIEQ
                     RVLNLLKQGYQSRVPLDDCTIKTICKAANKGDSLASEVIEYVGRHLGKTIAIAINLFN
                     PQKIVIAGEITEADKVLLPAIESCINTQALKAFRTNLPVVRSELDHRSAIGAFALVKR
                     AMLNGILLQHLLEN"
     gene            20942..21694
                     /gene="nagD"
                     /locus_tag="PN977_003028"
     CDS             20942..21694
                     /gene="nagD"
                     /locus_tag="PN977_003028"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide monophosphatase NagD"
                     /protein_id="HDI6043042.1"
                     /translation="MTIKNVICDIDGVLMHDNVAVPGAAEFLHGIMDKGLPLVLLTNY
                     PSQTGQDLANRFATAGVDVPDSVFYTSAMATADFLRRQEGKKAYVVGEGALIHELYKA
                     GFTITDVNPDFVIVGETRSYNWDMMHKAAYFVANGARFIATNPDTHGRGFYPACGALC
                     AGIEKISGRKPFYVGKPSPWIIRAALNKMQAHSEETVIVGDNLRTDILAGFQAGLETI
                     LVLSGVSSLDDIDSMPFRPSWIYPSVAEIDVI"
     gene            21951..23615
                     /gene="asnB"
                     /locus_tag="PN977_003029"
     CDS             21951..23615
                     /gene="asnB"
                     /locus_tag="PN977_003029"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001724523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase B"
                     /protein_id="HDI6043043.1"
                     /translation="MCSIFGVFDIKTDAVELRKKALELSRLMRHRGPDWSGIYASDNA
                     ILAHERLSIVDVNAGAQPLYNQQKTHVLAVNGEIYNHQALRAEYGDRYQFQTGSDCEV
                     ILALYQEKGPEFLDDLQGMFAFALYDSEKDAYLIGRDHLGIIPLYMGYDEHGQLYVAS
                     EMKALVPVCRTIKEFPAGSYLWSQDGEIRSYYHRDWFDYDAVKDNVTDKNELRQALED
                     SVKSHLMSDVPYGVLLSGGLDSSIISAITKKYAARRVEDQERSEAWWPQLHSFAVGLP
                     GSPDLKAAQEVANHLGTVHHEIHFTVQEGLDAIRDVIYHIETYDVTTIRASTPMYLMS
                     RKIKAMGIKMVLSGEGSDEVFGGYLYFHKAPNAKELHEETVRKLLALHMYDCARANKA
                     MSAWGVEARVPFLDKKFLDVAMRINPQDKMCGNGKMEKHILRECFEAYLPASVAWRQK
                     EQFSDGVGYSWIDTLKEVAAQQVSDQQLETARFRFPYNTPTSKEAYLYREIFEELFPL
                     PSAAECVPGGPSVACSSAKAIEWDEAFKKMDDPSGRAVGVHQSAYK"
     gene            23996..24072
                     /locus_tag="PN977_003030"
     tRNA            23996..24072
                     /locus_tag="PN977_003030"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:24030..24032,aa:Met,seq:cat)
     gene            24081..24165
                     /locus_tag="PN977_003031"
     tRNA            24081..24165
                     /locus_tag="PN977_003031"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:24115..24117,aa:Leu,seq:tag)
BASE COUNT         6037 a         6088 c         6007 g         6071 t
ORIGIN      
        1 tgcaggcccg tctgcgcgtc gctttacgcc gccactccgc caccaccgcg cccgatccgc
       61 tggtgaaatt ttccgatgtg accgtcgatt tagccgcccg cgtgattcac cggggtgagg
      121 aagaggtgca tctcacgccg attgaattcc gcctgctggc cgtgctgctc aacaacgccg
      181 gaaaagtgct cacccagcgc cagttactca atcaggtgtg gggaccaaac gcggtcgaac
      241 acagtcatta tttgcgtatt tatatggggc atctgcgaca aaaactggag caagatcccg
      301 cccgcccccg ccatttcatt actgaaaccg gtattgggta tcggtttatg ctttgaatat
      361 taattttaat acatcctgcc ttttattaat taaagccgta ataataaata cggcttttta
      421 tcttaaacaa cacacaaaaa taacaattca atattttata ttactgagta aaaagttcat
      481 cattaaataa attaagattg atcatttttt attgatcacc ttcacagttc aacagtattt
      541 cctggataga ataccttcac cttcattcac atcaggaaag gtaaattaaa tggaaaataa
      601 cagccgcact atgccccata taaggcggac aactcatatt atgaagtttg ctcatcgcaa
      661 tagcttcgac tttcacttct tcaatgcccg ttagtctacc gactaagggc acttcagcgt
      721 acaggtcttc ctgactctct gtattacagg catatgcctg tattccgcag tgctcattct
      781 ctgttaaccc ttgtattatc agccaccgga ctgataaggg tttctgcatt ctgaaaaaag
      841 caaacttctg attaatttat cagcggggta actttgcttt ttttccggca cgatcgattt
      901 ctcattcgag aaattgagga cctgctatta ccaaaaataa agagttggaa aatgtcaaaa
      961 ttaaaaattg cggttagtga ttcttgcccg gactgtttta ccacgcagcg agaatgtatc
     1021 tatattaatg aaaacagtaa tatcgatgtg gcggcaatag ttttatcgct caacgatgtt
     1081 acacgcggaa aactcgatga aatcgatgcc acgggttatg gcatcccggt atttattgct
     1141 actgaaaatc aagaacgtgt acccgcagag tatttgcccc gtatttcggg tgtctttgag
     1201 aattgcgaat cgcgacgaga attttatggt cgtcagttag aaaccgcagc cagccattat
     1261 gaaactcaac tgcgcccgcc cttcttccgc gcactggtcg attatgtcaa tcagggtaac
     1321 agcgcgttta attgccccgg tcatcagggc ggcgaatttt tccgtcgcca tccggcgggg
     1381 aatcagtttg tggaatactt tggtgaggcg ctgttccgtg ctgacttgtg caacgcggat
     1441 gtggcaatgg gcgatctgct gattcacgaa ggcgcgccat gcattgcaca gcaacatgcg
     1501 gcaaaagtgt ttaatgccga taaaacatac ttcgttctaa atggcacttc atcttctaac
     1561 aaagtggttt taaacgccct gttaacaccg ggggatctgg tgctgtttga ccgtaataac
     1621 cataaatcta atcaccacgg agcgttgcta caggctggcg caacaccagt ttatctggaa
     1681 accgcacgca acccgtatgg ctttatcggt ggtattgatg cccactgctt cgaagaaaat
     1741 tacctgcgcg aactgattac agaagtcgca ccgcagcggg caaaagaggc gcgtcctttc
     1801 cgcctcgccg ttattcagct aggcacttac gacggtacga tttataacgc ccgtcaggtg
     1861 gtggataaaa ttggtcatct gtgtgactac atcctctttg actccgcatg ggtcggctat
     1921 gagcagttta tcccgatgat ggcggactgt tcaccactgt tgctggatct taatgagaac
     1981 gatccgggta ttctggttac gcaatctgtg cataaacagc aggctggttt ttcccagact
     2041 tcacaaatcc ataaaaaaga cagccatatc aaagggcaac agcgttatgt accgcacaaa
     2101 cgcatgaaca acgcctttat gatgcatgcc tccaccagcc cgttctatcc gctgtttgcc
     2161 gcactggata tcaacgccaa aatgcatgaa ggcgtcagcg gtcgtaatat gtggatggat
     2221 tgtgtggtaa atggcattaa tgcccgcaaa ctgatcctcg ataactgtca gcatattcgt
     2281 ccattcgtac ctgaactggt ggatggtaaa ccctggcagt cgtatgaaac agcgcaaatt
     2341 gcggttgatc tgcgcttctt ccagtttgta ccaggtgagc actggcattc ttttgaaggc
     2401 tatgcagaga atcaatactt tgtcgatccg tgcaaactgt tgctgacaac cccaggtatt
     2461 gatgcacgta acggcgaata tgaagcgttc ggtgtacccg cgacgattct tgctaacttc
     2521 ctgcgcgaaa atggcgtagt gccggaaaaa tgcgatctta actccatcct ctttctgctg
     2581 actccggcag aagatatggc caaacttcag caacttgctg ctctgctggt acgcttcgaa
     2641 aaactgcttg agtccgacgc gccattatca gaagtactgc cttccatcta caaacagcat
     2701 gaagagcgct acaccggtta taccctgcgt cagttgtgtc aggaaatgca tgatttgtat
     2761 gcccgtcaca acgtgaaaca actgcaaaaa gagatgtttc gtaaggaaca cttcccacgt
     2821 gtcagcatga atccacaaga agccaactac gcctatttac gcggtgaagt ggagctggtt
     2881 cgtctgccgg atgcagaagg ccgtatcgct gcagaaggtg cgcttcctta tcctccggga
     2941 gtgctgtgtg ttgttccggg tgaaatctgg ggtggtgctg ttctgcgtta cttcagcgct
     3001 ctggaagaag ggatcaacct gctgccaggt tttgcaccgg agcttcaggg tgtctatatc
     3061 gaagaacatg atggtcgtaa gcaagtttgg tgctatgtca tcaagcctcg tggtgcgcaa
     3121 agcaccctgc tgaaagggga aaaattatga gtcaggctaa atcgaacaag atgggcgtcg
     3181 ttcagttaac catactgacg atggtcaaca tgatgggctc cggtatcatc atgctgccga
     3241 caaagcttgc cgaagttggg acaatctcaa ttatctcctg gctggtgaca gccgtaggct
     3301 caatggcact ggcgtgggca ttcgcaaaat gcggtatgtt cagccgtaag gcaggtggta
     3361 tgggcggtta tgcggagtat gctttcggta aatccggtaa ctttatggcg aactacacct
     3421 acggtgtctc gctgctgatt gctaacgtcg cgattgctat ttcggcggtt ggttacggca
     3481 ccgaactgct cggcgcgagt ttgtcgccag tgcagattgg tcttgcgacc atcggggtgc
     3541 tgtggatttg taccgtggct aacttcggtg gtgcgcgcat taccgggcaa atcagtagca
     3601 ttactgtgtg gggggtaatc attccggtcg tcggtctgtg cattatcggc tggttctggt
     3661 ttagcccgac gctgtacgct aactcatgga accctcatca tgtaccgttc tttagcgctg
     3721 taggttcctc catcgccatg acgttgtggg ctttcctcgg tctggagtct gcctgtgcca
     3781 acgccgaagt agtggaaaac ccggaacgta acgtgccaat agcagtcctt ggcggtacgt
     3841 taggtgcggc agtgatttat atcgtctcca ccaacgtgat tgccgggatt gtgcccaata
     3901 tggagctggc aaattcaacg gcaccatttg gcctggcctt cgcacagatg ttcacaccag
     3961 aagtgggtaa agtcattatg gcgctgatgg tgatgtcctg ctgcggttca ctgcttggct
     4021 ggcagttcac cattgcccag gtgtttaaat cttcatctga tgaaggctac ttccctaaaa
     4081 ttttctcccg tgtaaccaaa gtggatgcac cggttcaggg aatgttgatc attgtgatta
     4141 ttcagagtgg attggcactg atgaccatta gcccgtcact gaacagccag ttcaacgtgc
     4201 tggttaacct ggccgtggtg accaatatca tcccgtatat tctgtcgatg gcagcattag
     4261 ttattattca gcaggtcgcc aatgtgccac cgtcaaaagc gaaagtagca aactttgttg
     4321 cctttgttgg tgcgatgtac agtttctatg cgctgtactc atccggggaa gaagccatgc
     4381 tgtacggttc catcgtgacc ttccttggtt ggacactgta tggtctggta tctccacgct
     4441 ttgaactgaa aaataaacac ggttaaaaaa agcgaaataa aaaagggcga ttgaatgatc
     4501 gccctttctg tttgttgaca aagagttcgt tatttatgcc gcatccggca ggtgtagggt
     4561 ccaaacgcgg tcgaacacag caactatttg cgtaattata tgaggcattt gcgacaaaaa
     4621 ctggaacagg atctcgtccg ccccgccatt tcattactga aaccggtatt gggtatcggt
     4681 ttatgctttg aaaaaagtgg caatgtatgg ataaagtcat tttttattca aatataagga
     4741 acttaatatt taaaaaatgt cccatacaat gcctcatcat aaatgacacc gtagcacaca
     4801 aggagcaagt agcgatgcgt gaagtgagtc agttagtaaa tactgaagat tcagcctggc
     4861 caataatcca gaattggtta aaagaagcca caaaccatgc cgaactctta ccggttaata
     4921 aagatttagc cgaaaccgca ctttatcaat tacaggttac aacaaaatca ccgatgggtg
     4981 cgcttgtcta tggctccgga ggtctgctga ttgataacgg ttggttacgc atagcaggct
     5041 ctggtcaccc ccggttacca cgagatccag caagttggac acaacgccct gaattcgcaa
     5101 gcgtgcgggc tttacccatc gccgatgatg tcgcaggggg aatttttgct cttaatggtg
     5161 gcgatcttgg ggaagatact ggctgcgttt attattttgc gccagatacg ctgaattggg
     5221 aaagtctgga ggttggatat tctgagtttc ttcagtgggc attatccggt gatttagata
     5281 ccttctatga aaatgtacgc tggccgcaat ggcaagaaga cgtaataaaa ttatcggcaa
     5341 cggaagcctt cactttctac cctttcttat gggtacaatg tgaagaagca agaacaagaa
     5401 aagttatttc gcttactgaa ctctgggaaa tgcagtatca aatgaaagaa acaatcacgc
     5461 aataagacat gttcaggtcc gggtttcatt cccggactcg ctattttaat gtgtgtttat
     5521 cgctttcaat tcttgctggc aattttctga ctgacagcgt cgcaactcaa taatccctac
     5581 atgcatcatc gagccaaaaa atccgctttc gctgacataa tcctcattaa ctagcggtcg
     5641 agtgccgggc ttaccctggc aaatccccat aaaaaatgag gtgtagcatt gccattcagg
     5701 atttaaaccg gtcttttctt gtgctgacat atcatgaaga taagtaagtt ctttttcgtt
     5761 cccgcctgac aactgccagc ggcgggtatt atcaggtaag ttatcgccac ggcaatttgt
     5821 cttacaccgg gaaggcgcaa tatttaccgt aatgctcccc gttaatcctc tggttttatc
     5881 tttaggggtc agtaaccagt caccagctaa tgatgattgt tcaggttggg cgcatgctgt
     5941 cagcagtagt gtggcggtaa ttaatgccac tctgacgacc agactccttt tggtcttcat
     6001 tttgaattcc cttgagctat tcagtttgtt ggcaaagtgc tcgttgttca tgccggatgc
     6061 ggcgtgaacg tcttatccgg cctacaaaat cgcgtaaatt caatatattg caccatcttc
     6121 ccaagcctga taagcgtagc gcatcaggca gtcataagta aaaaaaggcg atcttctgac
     6181 cgcccttttt cttattaaat gtgtttacgc gtttttcaga acttcgctaa caatctcaac
     6241 cgcttctttc tcaatctgct tgcgatgttc ttccccgagg aagctttcgc agtagatttt
     6301 gtatgcgtct tctgtgcctg acggacgcgc ggcgaaccag ccgttgtcag tcatcacttt
     6361 cagaccgcca atagaagcac cattacccgg agccgccgtc aggcgtgcgg tgatcgggtc
     6421 acctgccagg gtgctggagc tcaccatttc cggagacagc ttagacagcg ccgctttttg
     6481 tgcggaagtc gcagctgcct gcaaacggtt atagctcggc gcaccaaagc gttctgccag
     6541 ttcgttgtag tgttcctgcg ggttcttacc agtgacagcg gtgatttccg ccgccagcag
     6601 acacatgatg atgccgtctt tatcagtcga ccacggcgtg ccgtcgaaac gcaggaagga
     6661 agcccctgcg ctctcttcgc cgccaaagcc gaagctgcca tcgaacagac catcaacaaa
     6721 ccatttgaaa cctaccggga cttctaccag cttacggccc aagtcattga ccacacggtc
     6781 gatcatcgca gatgaaacca gcgttttacc gacagcaaca tctttgcccc actgcggacg
     6841 gtgctggaac aggtagttga tcgccaccgc caggtagtgg ttcggattca tcaaacctgc
     6901 cggagtgacg ataccgtgac ggtcataatc cgggtcgtta gcaaacgcca gatcgaactt
     6961 atcacgcagt gccagcagac ccgccatcgc acactcggag gagcagtcca tacggatcgc
     7021 accgtcttta tcgaggtgca taaagcggaa ggtttgatcg acctgatcgt taacgatggt
     7081 caggttgagg ttgtaatact cgccaatgcg cttccagtat tcgataccgg aaccgcccaa
     7141 cggatcaacg cccagcgtca ggcccgcttt ctgaatcgcc gccatatcaa cgatatcggc
     7201 cagaccttcc acaaacggct gcaccagatc ctgctctttc acatgaccgg atgccatcgc
     7261 ttcgtcgagg gagatacgct tcacgccttt caggccatcg gccagcagtg cgttggccct
     7321 gtcttccacc actttggtga cgttggtatc agccgggcca ccatttggcg ggttgtactt
     7381 gataccacca tcttccggcg ggttatggga cggtgtaatc acgataccgt ctgccagcgg
     7441 gccacctttt ttattgtgaa ccaggatggc attggaaacg gcaggcgttg gagtgaagcc
     7501 attgttttcc tgcacaataa catcaacgcc gttcgctgcc agcacttcca gaacggaaat
     7561 aaacgcaggc tcggacaggg cgtgagtatc tttacccaca tagcaagggc cagtgatgcc
     7621 gtttttcgca cgttcttcag caattgcctg agcgatcgcc agaatgtgcg gctcgttaaa
     7681 gctgtggcgc gctgcactgc cacggtgacc ggaagtaccg aatttcaccg cgtgctccgc
     7741 attccctgct tctggtttca gtacataata ttgcgccgtc agttgggcga cgttaatcaa
     7801 atcactctgt tgtgcaggtt ggcctgcacg attgtggatt gccattgctt tgtcctttgt
     7861 ctgcaacgtt ttagatagtt ccgcaaacct tctcaatcaa ttccgccggg aattgcatcg
     7921 actgcatgat gtgttcgatc atgctgcatt tacggccggt gttggtgttg gtgatcaccc
     7981 aatacggcgt accaggcaca tgtttcggct tggtctgatt accatttttc agcagcgttt
     8041 gttcatctgc cgcaaagtaa acgcgtgtac gaccgtgcaa cgattccgtt gcttcggcaa
     8101 acgcctgggc gtcaagagaa tatagtgtag acaacagcag cataaagcga ttgaccgctc
     8161 gcttttgctc tgcgtattca tccgaaagca gaagttcacg cattgcgcga accttgtctt
     8221 taatcgtttt gaccggcttc gcttcgacga tagcaggtga cgcaacgcga acctctttcg
     8281 tcaccggagc agcaggctgt gatgcggcgg aaaatttcaa catacgccgt aaaatgtcgg
     8341 atgcgctctc gccgatatgc ttagtgtggc tggcaatata gctgtagagt tcatcatcaa
     8401 cttcaatcgt tttcatctta atccagtgca gtgtcttagc tgaatacaag tcgccaggag
     8461 tatatgaata aatcccggca gcggatagcg tcaagcctgg cttgcagaaa aactcggaat
     8521 aaaccttatt tgttgcagcc ctgtggcaca atggtcaaca taatacccta acccgacaac
     8581 gcgaaaaaaa gaactttgcc atgaaattga atatccgcgc gcaaactgca caaaaccagc
     8641 acaataattc tcccatcgtt cttgtccatg gtctgtttgg cagcctcgac aaccttggcg
     8701 tactggctcg cgatctggta aacgatcaca atatcatcca ggttgatatg cgtaaccacg
     8761 gtctttcacc gagagatccg gtaatgaatt acccggcgat ggcgcaggat cttgttgata
     8821 ccctggatgc acagcagatc gacaaagcaa catttatcgg tcactccatg ggcggtaaag
     8881 cggtaatggc tcttactgca ctagcccccg atcgcattga caagctggtg gcgattgata
     8941 ttgcgccggt cgactatcac gtacgccgtc atgatgagat tttcgcggcc atcaacgcag
     9001 tcagtgaatc agacgcacaa acacgccagc aagcagcggc aataatgcgc cagcatctta
     9061 atgaagaagg ggtgattcag tttctgttga aatcttttgt tgacggggag tggcgcttta
     9121 acgtgccagt attgtgggat cagtatccgc atattgtggg ttgggagaaa atcccggcat
     9181 gggatcatcc taccctgttt atccctggcg gcaattctcc gtatgttagc gagcagtacc
     9241 gtgatgattt actggcacaa tttccacagg ctcgggcgca tgtgattgca ggcgcgggtc
     9301 actgggtcca tgctgaaaaa ccggatgcgg tattacgcgc tatccgtcgc tatctcaatg
     9361 attaacgcaa ctgattaaaa acccagcgtc cgcgtcgctt tccgcgcgtg ggcgttggcg
     9421 tgactcatcg cgctgatgta ttatggcgcg ctatccatcc gggcaggcgc ctggctgatt
     9481 gtttcccccg aagtcaccaa gatcatggcc aaagaacaaa cggaccgtac gacattagat
     9541 ctgttcgcgc acgagcgtcg accgggacga ccgaaaacta atccgctttc gcgcgatgaa
     9601 cagctgcgta ttaataaacg caaccaacta aaacgcgaca aagtacgtgg ccttaagcgt
     9661 gtcgaactga agttgaacgc ggaagctgtc gaggcactga acgagctggc ggaatcgcgc
     9721 aatatgagcc gtagcgagct gatcgaagag atgttaatgc agcaactggc ggcgctgcgt
     9781 agtcagggca tcgtttaaat ttccactttc atgtagcaca gtgtgcagtc ctgctcgttt
     9841 gctgattccg caagactgcc tgttctgcta tgattgcctt tatccgtggg caattttcca
     9901 cccccatttc aataagtttc aagaggttat ttcactcatg gctatcactg gcatcttttt
     9961 cggcagcgac accggtaata ccgaaaatat cgcaaaaatg attcaaaaac agcttggtaa
    10021 agacgttgcc gatgtccatg acattgcaaa aagcagcaaa gaagatctgg aagcatatga
    10081 cattctgctg ctgggcatcc caacctggta ttacggcgaa gcgcagtgtg actgggatga
    10141 cttcttcccg actctcgaag agattgattt taatggtaaa ctggttgcgc tgtttggttg
    10201 tggcgaccag gaagattacg cagaatactt ctgcgatgca ttgggcacca tccgcgacat
    10261 cattgaaccg cgcggcgcaa ctatcgttgg ccactggcca accgcgggct accacttcga
    10321 agcgtcaaaa ggtctggctg atgatgacca ctttgtcggt ctggctatcg acgaagatcg
    10381 tcagccggaa ctgaccgctg aacgtgtaga aaaatgggtt aaacaaattt ctgaagagct
    10441 gcatctcgac gaaattctca atgcctgatg tgatgcggcg tagactcatg tctacgccgt
    10501 attaatagat aataccaatc aaaataattg ctacaaattt gtaacttttg ctgttgtacc
    10561 tgtacaatgt cccggtgttc aagtggcctt gccgttgtaa atgtaagctg tgccacgttt
    10621 ttattaacaa tatttgccag ggacttgtgg ttttcattta ggcgtggcaa ttctataatg
    10681 atacgcatta tctcaagagc aaattctgtc acttcttcta atgaagtgaa ccgcttagta
    10741 acaggacaga ttccgcatga ctgataacaa taccgcccta aagaaagctg gcctgaaagt
    10801 aacgcttcct cgtttaaaaa tcctggaagt tcttcaggag ccggacaacc atcacgtcag
    10861 tgcggaagat ttatacaaac gtctgatcga tatgggtgaa gaaattggtc tggctacggt
    10921 atatcgcgta ctgaaccagt ttgacgacgc tggtatcgtc acccgccaca attttgaagg
    10981 cggtaaatcc gtatttgaac tgacacagca acatcaccac gatcacctga tctgcctcga
    11041 ctgcggcaag gtaatcgaat ttagtgatga ttccatcgaa gcgcgtcagc gtgaaattgc
    11101 cgcaaaacat ggcattcgcc tgactaacca cagtctctat ctttacggtc actgtgccga
    11161 aggcgattgc cgcgaagatg agcacgcgca cgaaggcaaa taagccagcc tgaacgagaa
    11221 aagccaacct gcgggttggc ttttttatgc aagggaaata agaaaagcga gagttacagc
    11281 tctcacttat ttgttattac tgcgaatttc ctgccagacc ttatcacaat cggctttcac
    11341 cgcctgatca tttccggttt gcgttgcgcg cagacactgc tgataatcca gtacgcggac
    11401 actttcctgg tcggcaaact gctgatgttg tttctctttc ttcagcacgt ttaacacgct
    11461 ctggcaggct tcaatttttt ctggcgaacc ttgtgcggtg ttgatacagg cgctgtatgc
    11521 ctctttcaga cgactgtctt cctgtggcgc ggttgattgc acacaagcta ccagccccga
    11581 tgccattatg gcgatgagaa tcagtttttt catagcaaat tcctcaaacg tgcggcaggc
    11641 acaaaacctg ccgcgtcggg catcagaaga tggtgaatgg tgcgattacc ataaatttca
    11701 cgtcacgctc atcctggaag atgttgccgt aaccaccgcc ccagcttggg atgtcggagt
    11761 ggttgtcgta ttcggtgaag tgcagtttga acatcgtgcc tttggcgcga ccgtcctgaa
    11821 tggtgtacac cgcatccagg ctgtaggcag actcttcaat agtccggttt ttatcgtagt
    11881 acgcatccgg attgctctgc caggtcgcag gtttagcatc ccatgcgtaa acgtaggaag
    11941 cgccgatggc gaagcctgga agattccagt ttttcaggtc atacatcgca ccgaagaaga
    12001 tcgctttttc gccgttggcg ttgaagtcgg aacggttatc ccaccagata tccaggcgac
    12061 cgttcgagga agcgtaggtt ggagtcatac gttgcaggaa gtatccctgc tgaccgtcag
    12121 ccttaaccca ggtaccttcg aggcgcaaat ccactacgtc agccgcccgg taaccaaagg
    12181 tcaacgcctg cagccaggcg gtgccgtcat aaagatcgtt gacgctgcga tcgtcaactt
    12241 tatcgcgcgt accatagaac tggtagctgg tggttaacgg gctaccggcg atatcaaatt
    12301 tgtagctggc tttggcaaaa tattgatcga tatacccttc ggcctgacca aacgccgctt
    12361 ccagtacgaa gttatttttg aagtcgtact tcgccccaag ggagtgtaga taatcaactt
    12421 tggtggtttt atcgttctga taaaactcat ccatttccag atgccacggt gctttgtatt
    12481 cgttggtcca catgtaggag aaactcaacg caccagcatc gccataatca aaattcgccc
    12541 cggcttccgc cccctgataa gtacccggca taaagctcca gtgcggcgct aacagcgttt
    12601 gaccagttgg ctgaatgtaa cctgccctcg cccaaaccgg accgtattta aatttagccg
    12661 ccgctttata cagacttata ccgcttttat cgccggacca gtcttcgtca taggctttat
    12721 tactttttga aaacgcgatt tcgttcgggt gagagctgtc gccgttttct gccatttcaa
    12781 tcgctgtaaa cgcggcaata tccagaccga acatatcagc ggcatagcca gactgaaaat
    12841 cgagattagc gttccaggtg gagtgagaaa ggttggtttt atatttgtcg ccgtcggtaa
    12901 catctttacg gtcgcgttca cgctgccagt aatagatacc gccggttaag gttgaatcgt
    12961 cgatgaatcc ttcggcgcga gcctccggaa ttgccataaa gcccgacata gctgtaacac
    13021 cggcgatagc cagcgccagc gtactacgtt tgccactaaa cgcacgcatg ggttaatcct
    13081 ctttgacgta taaattgctg caccaaaggt gaacagcgaa aaaaataaaa attaaaaaag
    13141 tagcgttgag aaaagtctcg ctgactacag gaacagacta tttttcgcga cgcgaattat
    13201 caatctcttt ctgtaaccag aatgtaataa catgagcaac cgcacatatt ttgatgattt
    13261 ttgttacaag gcttaagttt gcagttgaaa ggcttgcaga agaataaaaa cgtgaaattc
    13321 cagcaagatg aaagcggtta cattttattt aattatgatt tgtaatttta aatttgcttt
    13381 atgaataaat tacgaagcgg aattaattcg cgaagaaaag cagaaaaaaa acgccgcgga
    13441 gcgcggcgtt tgggtttgtt tgctattttt tgccggatgc ggcgtgaacg ctctatccga
    13501 caacgtaaac ttactcgcct actttcgccc aggtatcacg cagcccaacg gtgcggttaa
    13561 ataccggttt ttccgccgta gaatggcggc tgtcgaggca gaagtaacct tcacgctcaa
    13621 actggaatgc tttacccgca accgcatctt tcagcgacgg ttcagcaaag ccctgtttga
    13681 tcaccagcga ttccgggtta atcaccgaca ggaaatcatc cgcagcacca gggttcggca
    13741 cgctgaacag acggtcatac aaacggattt caaccggcag cgcatgtgcc gcgctcaccc
    13801 agtgaataac acctttgact ttacgaccat ctgccggatc tttgcttaag gtgtcggcgt
    13861 cataagtaca gaagatggtg gtgatattgc cttcggcatc tttctccacg cgttctgcct
    13921 taatgacgta agcattacgc agacgcactt ctttacccag caccagacgt ttgtactgct
    13981 tgttagcttc ttcgcggaaa tcggcgcgat cgatccaaat ctcaccgcta aatggcacct
    14041 ggcggctgcc catttccggt ttattcggat ggttcggcat ggtaaccatt tcgccttcgc
    14101 cctggtagtt ttcgataacc agtttcaccg gatcgataac cgccattgcg cgcggcgcat
    14161 tttcgttgag atcttcacgg atgcaggatt ccagcgacgc catctcaatg gtgttgtcct
    14221 gcttggtcac gccgatgcgt ttgcagaact cacgaataga agctgcagtg taaccacgac
    14281 gacgcagacc ggaaatggtc ggcatacgcg ggtcatccca gccttcaacg tgcttgtcgg
    14341 tcaccagcag gttcagctta cgcttggaca tcacagtgta ttccagattc aggcgcgaga
    14401 attcgtactg gcgcgggtga acaggaatgg tgatgttgtc cagcacccag tcgtacaggc
    14461 gacggttgtc ctggaactca agcgtacaca gagagtgcgt aataccttcc agcgcatcgc
    14521 tgatgcagtg ggtaaagtcg tacatcgggt agatgcacca cttgttgcca gtctggtggt
    14581 gttcagcaaa cttaatgcgg tacagcaccg gatcgcgcat cacgataaac ggcgaagcca
    14641 tgtcgatctt cgcacgcagg caggctttac cttcttcaaa accgccggta cgcattttct
    14701 cgaacagcgc caggttctct tcaacgctgc gatcgcggta cgggctgttt ttacccggct
    14761 gcgtcagggt gccacggtat tcgcggatct gttccggtgt cagttcatca acgtacgcca
    14821 ggcctttatt gatcagttcg atcgcatagg cgtggagctg atcaaaataa tcggaggagt
    14881 aacggacgtt accagaccag tgaaaaccta accactcaac gtcgtttttg atcgactcaa
    14941 catactcgat atcttctttt accgggttag tgtcgtcgaa acgcaggttg cactggcctt
    15001 tatagtcctg ggcgatcccg aagttcaggc agatagattt cgcatggcca atatgcagat
    15061 agccattcgg ctccggcggg aaacgggtgt gtactgtggt gtgcttacca ctggccagat
    15121 cttcatcgat gatctgacga ataaagttag tcgggcgggc ttctgcctca ctcatcgtgg
    15181 attcctcaaa gcgtaaacaa cgtataacgg cgtatgatct tataagcggg acaggctgac
    15241 aactgttagt tagtgaaaat cgacaatctc actgagatta tggggattat tgatgaaaaa
    15301 aaaagcggca gagaagatct ccaccgcttg tcattgttgg atgcgacgct caagcgtcgc
    15361 atcaggcata aagaagatta ctttttgatt tcatacagcg gcgtttgacc cgccacgaca
    15421 tggccctgag ctttaatgat caagccgctg aaatcgtcga tattgctgca aaccaccggg
    15481 ctaatcatcg agcgggcgtt ttcgttcaga taatccagat ccatttccag aatcggttgc
    15541 cctgcgctta cttgcgcgcc ctcttccacc agacgtttaa agcctttacc ttccagcgct
    15601 acggtgtcga tacccatatg gacgacaatc tccgcgcctt tttcagtttc caggcagaat
    15661 gcgtggttgg tgttgaagat tttcacgata gtccctgcgg ctggtgatac gacgatttta
    15721 tctgtcggtt tcaccgccac accgtcaccc accgctttgc tggcgaatgc ttcgtcagga
    15781 acctgatcca gtgccacaac atcaccggta atcggcgata ccagctccgc gatagatact
    15841 gcgtttggta cagcctgcgg ttttgctaca ggcgcggcag ttgccggagc agtttcagca
    15901 gaggcagcgg ctaccggacc gcgggcaacg actttcttca tcgcatcgcc gatggattct
    15961 gctttcgcgc caacaatcac ctgaatagtc tgtttgttca gtttcactac cccagaagca
    16021 cccagacgtt tacacatcgt atcgttaacg cgggcagagt cagccactgt aaggcgcaga
    16081 cgggtgatac aggcgtcaat tgctttcagg ttgtcagtgc cgccaaccgc agcaatatag
    16141 ttggttgcca gttgattgag accttcttca gtattgctgt tagcctcttc agtaacgatc
    16201 tcgtcttctt tatcttcacg acctggcgtt ttcaggttga acatgcggat aaccaaactg
    16261 aacaccacga agtagatagc gaagaagaca acccccatca ccagcagcat ccagacgttc
    16321 tggctggcgg ccggcaggtt atacatcaac gcgtagtcga tagcccccgc agagaaagag
    16381 aagccggcat ggatacccag caacgttgcc acaaacaggc tgataccggt cagcagtgcg
    16441 tgcaggaggt acagcagcgg tgcaaggaac atgaacagga attccagcgg ctcagtcaca
    16501 ccggtcagga acgcagtaac agcaacagaa agcagcatcc cgccaaccat cggacggcgc
    16561 tctttcggtg ctgcaaagta catcgccagc gccgcacccg gcagaccgaa catcatgatc
    16621 gggaagaagc cggacatgaa catccccgcg gtgccgtcac cggcatagaa gcggttgatg
    16681 tcaccgtgaa aaaccgtacc cgccgcgttg gtgaattcac caatctggaa ccaggcgatg
    16741 gtgttcagta cctgatgcag accggttggg atcagcagac ggttgatgaa accaaagata
    16801 ccggaaccca gcgcgcccgc agaaacgatc cactcgccgc ctgcatggat agcgtgctgt
    16861 accggcggcc agacgtaacc aaaaatggcc gccagcacca ggcagaagaa gccggtggca
    16921 atcggcacaa agcgtttgcc gccgaagaaa ctcaggaagt ccggcagttt aatatcggac
    16981 caacggttat aagctgcgcc accaaccaga ccggtaatga tacccgccag tacacccatg
    17041 ttaatttctg ggttgatggt caccatcgct ttggttaaca caaagtaacc taccgcaccc
    17101 gccagtgccg ccgcacctgc gctgtctttc gaccagctgg atgccacacc gatggcgaag
    17161 attaatgcga ggttatcaaa aatcgcaccg cccgcctggg caataaacgc aacgttaagt
    17221 aaatctggct gaccgaatcg cagcaacagc gccgccaccg gcagcaccgc gatagggagc
    17281 tgtaacgccc taccgagtcg ctggaaaaaa cctaaaatat tcatcttatt ccccctacga
    17341 gaaccctatt tggctcgttt aaagccgtat ttttattttg ctgcaaattg tactgccgat
    17401 gttctgtaat cagattgtta gatcatctgc tacagagtgt gtgaaaattt aattcgtatc
    17461 gcaaattaaa cgcgtgtctt ttgtgagttt tgtcaccaaa tatcgttatc atcactccct
    17521 tttactggct aaaccagaaa acttatttta tcattcaaaa aatcaggtcg gattgacgcc
    17581 tgtctgcgca aatccaggtt acgcttaaag atgcctaatc cgccaacggc ttacatttta
    17641 cttattgagg tgaataatga gactgatccc cctgactacc gctgaacagg tcggcaaatg
    17701 ggctgctcgc catatcgtca atcgtatcaa tgcgttcaaa ccgactgccg atcgtccgtt
    17761 tgttctgggc ctgccgactg gcggcacgcc gatgaccacc tataaagcgt tagtcgaaat
    17821 gcataaagca ggccaggtca gctttaagca cgttgtcacc ttcaacatgg acgaatatgt
    17881 cggtctgccg aaagagcatc cggaaagcta ctacagcttt atgcaccgta atttcttcga
    17941 tcacgttgat attccagcag aaaacatcaa ccttctcaac ggcaacgccc cggatatcga
    18001 cgccgagtgc cgccagtatg aagaaaaaat ccgttcttac ggaaaaattc atctgtttat
    18061 gggcggtgta ggtaacgacg gtcatattgc atttaacgaa ccggcgtctt ctctggcttc
    18121 tcgtactcgt atcaaaaccc tgactcatga cactcgcgtc gcgaactctc gtttctttga
    18181 taacgatgtt aatcaggtgc caaaatatgc cctgactgtc ggtgttggta cattgttgga
    18241 tgccgaagaa gtgatgattc tggtgctggg tagtcagaaa gcactggcac tgcaggccgc
    18301 cgttgaaggt tgcgtgaacc acatgtggac catcagctgt ctgcaactgc atccaaaagc
    18361 gatcatggtg tgtgatgaac cttccaccat ggagctgaaa gttaagactt taagatattt
    18421 caatgaatta gaagcagaaa atatcaaagg tctgtaattg ttatccctgc cctggctcct
    18481 tgctcagggc aatatttttt aaaatcgggg gtcagaatgt atgcattaac ccagggccgg
    18541 atctttaccg gccacgaatt tcttgatgac cacgcggttg ttatcgctga tggcctgatt
    18601 aaaagcgtct gtccggtagc ggaactgccg ccagagatcg aacaacgttc actgaacggg
    18661 gccattctct cccccggttt tatcgatgtg cagctaaacg gctgcggcgg cgtacagttt
    18721 aacgacaccg ctgaagcagt cagcgtggaa acgctggaaa tcatgcagaa agccaatgag
    18781 aaatcaggct gtactaacta tctgccgacg cttatcacca ccagcgatga gctgatgaaa
    18841 cagggcgtgc gcgttatgcg cgagtacctg gcaaaacatc cgaaccaggc gttaggtctg
    18901 catctggaag gtccgtggct gaatctggta aaaaaaggca cccataatcc gaattttgtg
    18961 cgtaagcctg atgccgcgct ggtcgatttc ctgtgtgaga acgccgacgt gattaccaaa
    19021 gtgactctgg caccggaaat ggttccagcg gaagtcatca gcaaactggc aaataccggg
    19081 attgtggttt ctgctggtca ctccaacgcg acgttgaaag aagcgaaagc cggtttccgc
    19141 gcggggatta cctttgccac ccatctgtac aacgcgatgc cgtatattac cggtcgtgaa
    19201 ccgggcctgg cgggcgcgat cctcgacgaa gctgacattt attgcggtat tattgctgat
    19261 ggcctgcatg ttgattacgc caacattcgt aacgctaaac gtctgaaagg cgacaaattg
    19321 tgtctggtta ccgatgccac cgcgccagca ggtgccaaca ttgaacagtt catttttgcg
    19381 ggtaaaacaa tatactaccg taatggactt tgtgtggatg agaacggtac gttaagcggt
    19441 tcatccttaa ccatgattga aggcgtgcgt aatctggtcg aacattgtgg tatcgcactg
    19501 gatgaagtgc tgcgtatggc gacgctctat ccggcacgtg cgattggcgt tgagaaacgt
    19561 ctcggcacgc tcgccgcagg taaagtagcc aacctgactg cattcacacc tgattttaaa
    19621 atcaccagga ccatcgttaa cggtaacgag gtcgtaactc aataagagaa agtatgacac
    19681 caggcggaca agctcagata ggtaatgttg atctcgtaaa acagcttaac agcgcggcgg
    19741 tttatcgcct gattgaccag tacgggccaa tctcgcggat tcagattgcc gagcaaagtc
    19801 agcttgcccc cgccagcgta accaaaatta cgcgtcagct tatcgaacgc gggctgatca
    19861 aagaagttga tcagcaggcc tccaccgggg gccgccgcgc tatctctatc gtcaccgaaa
    19921 cccgcaattt ccacgcaatc ggcgtacggc ttggtcgtca cgacgccacc atcactctgt
    19981 ttgatcttag tagcaaagtg ctggcagaag aacattaccc gctgccggaa cgtacccagc
    20041 agacgctgga acatgccctg ctgaatgcca ttgctcagtt tattgatagc taccagcgca
    20101 agctgcgcga gctgatcgcc atttccgtga tcctgccagg gcttgttgac ccggacagcg
    20161 gtaaaattca ttacatgccg catattcagg tggaaaactg ggggctggta gaagcactgg
    20221 aagagcgttt taaagtgacc tgttttgtcg gccacgatat ccgcagtctg gcgctggcag
    20281 agcactactt cggtgcaagt caggattgcg aagactccat tctggtgcgt gtccatcgcg
    20341 gaaccggggc cgggattatc tctaacgggc gaatttttat cgggcgcaac ggcaacgtcg
    20401 gtgaaattgg ccatattcag gtcgaaccgc tgggtgaacg ctgccactgt ggcaactttg
    20461 gctgcctgga aactatcgcc gccaacgccg ccattgaaca acgggtgttg aatctgttaa
    20521 agcagggcta ccagagccgc gtgccgctgg acgactgcac catcaaaact atctgcaaag
    20581 ccgcgaacaa aggcgatagc ctggcgtcgg aagtgattga gtatgtcggt cgtcatctgg
    20641 gcaaaaccat cgccattgcc atcaacctgt ttaacccgca aaagattgtt attgccggag
    20701 aaatcaccga agccgataaa gtgctgctcc ctgctattga aagctgcatt aatacccagg
    20761 cgctgaaggc gtttcgtact aatctgccgg tggtacgttc tgagctggat caccgctcgg
    20821 caatcggcgc ttttgcgctg gtaaaacgcg ccatgctcaa cggtattttg ctccagcatt
    20881 tgctggaaaa ttaatgtgct tttatagtgg cgcttattgt tgtcaatatt ctgggtagtc
    20941 catgaccatt aaaaatgtaa tttgcgatat cgacggcgtg ctgatgcacg ataacgtcgc
    21001 cgtaccgggt gcagcggaat ttttgcacgg gattatggat aaaggcctgc cgctggtgtt
    21061 gctgaccaac tatccttcgc agaccgggca agatctggcg aaccgctttg ccactgcggg
    21121 tgtcgatgtg ccggacagcg tgttttatac ctccgcgatg gcgaccgccg atttcctgcg
    21181 tcgccaggaa ggcaagaaag cgtatgtggt gggcgaaggc gcactgattc atgaactgta
    21241 caaagccggt ttcactatta ccgatgtgaa ccctgatttt gtgattgttg gcgaaacgcg
    21301 ttcctacaac tgggacatga tgcataaagc agcctatttc gtcgctaacg gtgcacgctt
    21361 tatcgccacc aacccggaca cccacgggcg cggtttttat ccggcctgtg gcgcgttgtg
    21421 tgcaggcatc gaaaaaatct ccggacgtaa accgttctat gttggtaagc cgagtccgtg
    21481 gatcatccgc gcagcattaa acaaaatgca ggcgcattcg gaagaaacgg tgattgtcgg
    21541 cgataacttg cgcaccgaca ttctggcggg cttccaggcg ggtctggaga cgattctggt
    21601 gctttctggt gtttcatcgc tcgacgatat cgacagtatg ccgttccgcc caagctggat
    21661 ttacccgtcg gtcgctgaaa tcgacgttat ctgaatagca accacgcctc agggcgtggt
    21721 ttcttatttt tagccatcat taaaatcatc ctattcaata aaacccgccc aaaattatcg
    21781 atccatcaat aaatacgtca ccacacccta tcctttttca caaatcagca atcgccatta
    21841 cgttttttat ttttccaccg ccaaatcgct tgcgcacagt gctgctttgc ggcatttttt
    21901 taaacaagca aacacaacaa gcaacaaata ccaggttaac ggagaaggtt atgtgttcaa
    21961 tttttggcgt attcgatatc aaaacagacg cagttgagct gcggaagaaa gccctcgagc
    22021 tgtcacgcct gatgcgtcat cgtggcccgg actggtccgg tatttatgcc agcgataacg
    22081 ccattctcgc tcacgaacgt ctgtcaattg ttgacgttaa cgcaggggcg caacctctct
    22141 acaaccaaca aaaaacccac gtactggcgg taaacggtga aatctacaac caccaggcac
    22201 tgcgcgccga atatggcgat cgttatcagt tccagaccgg atctgactgc gaagtgatcc
    22261 tcgcgctgta tcaggaaaaa gggccggaat ttcttgacga cttgcagggc atgtttgcct
    22321 ttgccctgta cgacagcgaa aaagatgcct acctgattgg tcgcgaccat ctggggatca
    22381 tcccactgta tatgggctat gacgaacatg gtcagctgta tgtggcctca gaaatgaaag
    22441 ccctggtgcc ggtatgccgc acgattaaag agttcccggc ggggagctat ttgtggagtc
    22501 aggacggcga aatccgttct tattatcatc gcgactggtt cgactacgat gcggtgaaag
    22561 ataacgtgac cgacaaaaac gagctgcgtc aggcactgga agattccgtt aaaagccatc
    22621 tgatgtctga tgtgccttac ggtgtgctgc tatctggtgg cctggattcc tcaatcattt
    22681 ccgctatcac caagaaatac gccgcccgtc gcgtggaaga tcaggaacgc tctgaagcct
    22741 ggtggccgca gctgcactcc tttgctgtag gtctgccggg ttcaccggat ctgaaggcag
    22801 cccaggaagt ggcaaaccat ctgggcactg tgcatcatga aattcacttc actgtacagg
    22861 aaggtctgga cgccatccgc gacgtgattt accacatcga aacctatgac gtgacgacaa
    22921 tccgcgcttc aacaccgatg tatttaatgt cgcgtaagat caaggcgatg ggcattaaaa
    22981 tggtgctgtc cggtgaagga tctgatgaag tgtttggcgg ttatctttac ttccacaaag
    23041 cgccgaatgc caaagaactg catgaagaga cggtgcgtaa actgctggcc ctgcatatgt
    23101 atgactgcgc gcgtgccaac aaagcgatgt cagcctgggg cgtggaagca cgcgttccgt
    23161 tcctcgacaa aaaattcctc gacgtggcga tgcgcattaa cccacaggat aaaatgtgcg
    23221 gtaacggcaa aatggaaaaa cacatcctgc gtgaatgttt tgaagcgtat ctgcccgcaa
    23281 gcgtggcctg gcggcagaaa gagcagttct ccgatggcgt cggttacagt tggatcgaca
    23341 ccctgaaaga agtggcggcg cagcaggttt ctgatcaaca gctggaaact gcccgcttcc
    23401 gcttcccgta caacacaccg acctcaaaag aagcgtatct gtaccgggag atctttgaag
    23461 aactgttccc gcttccgagc gccgctgagt gcgtgccggg cggtccttcc gtcgcgtgtt
    23521 cttccgctaa agcgattgag tgggatgaag cgttcaagaa aatggacgat ccgtctggtc
    23581 gcgcggttgg tgttcaccag tcggcatata aataagacgt ataatattgc cccggagaaa
    23641 tccggggcaa tttcagttac tggggcgctt gttgattctg atatgcagta aggaaatgtt
    23701 ccgggtttgc cacatcgctg tttgacaact cattactgta ggccagtatt tcatagatta
    23761 atttcccacc actattgttg aattccgaat ttcatcctgt atgacaaaag aatggctgta
    23821 gggtggggaa aaaacacaaa gtaaacaata attgacgaat atagcgccac gctgttcgca
    23881 acctaaccaa acagtcactt tcgagcaatt ttccttgaaa aagaggttga cgctgcaagg
    23941 ctctatacgc ataatgcgcc ccgcaacgcc gataaggtat cgcgaaaaaa aagatggcta
    24001 cgtagctcag ttggttagag cacatcactc ataatgatgg ggtcacaggt tcgaatcccg
    24061 tcgtagccac catctttttt gcgggagtgg cgaaattggt agacgcacca gatttaggtt
    24121 ctggcgccgc aaggtgtgcg agttcaagtc tcgcctcccg caccattcac cagaaagcgt
    24181 tgtacggatg gggtatcgcc aag
//
LOCUS       DAMZFT010000075        23910 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.075, whole genome shotgun sequence.
ACCESSION   DAMZFT010000075 DAMZFT010000000
VERSION     DAMZFT010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 23910)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23910)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23910
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.075"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(79..2016)
                     /gene="sltY"
                     /locus_tag="PN977_003032"
     CDS             complement(79..2016)
                     /gene="sltY"
                     /locus_tag="PN977_003032"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase"
                     /protein_id="HDI6043044.1"
                     /translation="MEKAKHVTWRLLAVGVCLLTVSSVARADSLDEQRSRYAQIKQAW
                     DNRQMDVVEQMMPGLKDYPLYPYLEYRQITDDLMNQPAVTVTNFVRANPTLPPARTLQ
                     SRFVNELARREDWRGLLAFSPEKPGTTEAQCNYYYAKWNTGQSEEAWQGAKELWLTGK
                     SQPNACDKLFSVWRASGKQDPLAYLERIRLAMKAGNTGLVTVLAGQMPADYQTIASAI
                     ISLANNPNTVLTFARTTGATDFTRQMAAVAFASVARQDAENARLMIPSLAQAQQLNED
                     QIQELRDIVAWRLMGNDVTDEQAKWRDDAIMRSQSTSLIERRVRMALGTGDRRGLNTW
                     LARLPMEAKEKDEWRYWQADLLLERGREAEAKEILHQLMQQRGFYPMVAAQRIGEEYE
                     LKIDKAPQNVDSALTQGPEMARVRELMYWNLDNTARSEWANLVKSKSKTEQAQLARYA
                     FNNQWWDLSVQATIAGKLWDHLEERFPLAYNDLFKRYTSGKEIPQSYAMAIARQESAW
                     NPKVKSPVGASGLMQIMPGTATHTVKMFSIPGYSSPGQLLDPETNINIGTSYLQYVYQ
                     QFGNNRIFSSAAYNAGPGRVRTWLGNSAGRIDAVAFVESIPFSETRGYVKNVLAYDAY
                     YRYFMGDKPTLMSATEWGRRY"
     gene            2227..3894
                     /gene="ettA"
                     /locus_tag="PN977_003033"
     CDS             2227..3894
                     /gene="ettA"
                     /locus_tag="PN977_003033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017145039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HDI6043045.1"
                     /translation="MAQFVYTMHRVGKVVPPKRHILKNISLSFFPGAKIGVLGLNGAG
                     KSTLLRIMAGIDKDIEGEARPQPDIKIGYLPQEPQLNPEHTVRESIEEAVSEVVNALK
                     RLDEVYALYADPDADFDKLAAEQGRLEEIIQAHDGHNLNVQLERAADALRLPDWDAKI
                     ANLSGGERRRVALCRLLLEKPDMLLLDEPTNHLDAESVAWLERFLHDFEGTVVAITHD
                     RYFLDNVAGWILELDRGEGIPWEGNYSSWLEQKDQRLAQEASQEAARRKSIEKELEWV
                     RQGTKGRQSKGKARLARFEELNSTEYQKRNETNELFIPPGPRLGDKVLEVSNLRKSYG
                     DRLLIDSLSFSIPKGAIVGIIGPNGAGKSTLFRMISGQEQPDSGTITLGETVKLASVD
                     QFRDSMDNSKTVWEEVSGGLDIMKIGNTEMPSRAYVGRFNFKGVDQGKRVGELSGGER
                     GRLHLAKLLQVGGNMLLLDEPTNDLDIETLRALENALLEFPGCAMVISHDRWFLDRIA
                     THILDYQDEGKVEFFEGNFTEYEEYKKRTLGADALEPKRIKYKRIAK"
     gene            complement(4001..4168)
                     /locus_tag="PN977_003034"
     CDS             complement(4001..4168)
                     /locus_tag="PN977_003034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6043046.1"
                     /translation="MSFFDELKTSLEEAVEIKQGLKKPARVTRHEIEDAKAVVDRKRC
                     SRRIPHSVLNA"
     gene            complement(4202..5434)
                     /gene="nadR"
                     /locus_tag="PN977_003035"
     CDS             complement(4202..5434)
                     /gene="nadR"
                     /locus_tag="PN977_003035"
                     /EC_number="2.7.1.22"
                     /EC_number="2.7.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463436.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional transcriptional
                     regulator/nicotinamide-nucleotide
                     adenylyltransferase/ribosylnicotinamide kinase NadR"
                     /protein_id="HDI6043047.1"
                     /translation="MSSFDYLKTAIKQQGCTLQQVADASGMTKGYLSQLLNAKIKSPS
                     AQKLEALHRFLGLEFPRQKKTIGVVFGKFYPLHTGHIYLIQRACSQVDELHIIMGFDD
                     TRDRALFEDSAMSQQPTVPDRLRWLLQTFKYQKNIRIHAFNEEGMEPYPHGWDVWSNG
                     IKKFMAEKGIQPDLIYTSEEADAPQYMEHLGIETVLVDPKRTFMSISGAQIRENPFRY
                     WEYIPTEVKPFFVRTVAILGGESSGKSTLVNKLANIFNTTSAWEYGRDYVFSHLGGDE
                     IALQYSDYDKIALGHAQYIDFAVKYANKVAFIDTDFVTTQAFCKKYEGREHPFVQALI
                     DEYRFDLVILLENNTPWVADGLRSLGSSVDRKEFQNLLVEMLEENNIEFVRVEEDDYD
                     SRFLRCVELVREMMGEQG"
     gene            complement(5455..6837)
                     /gene="radA"
                     /locus_tag="PN977_003036"
     CDS             complement(5455..6837)
                     /gene="radA"
                     /locus_tag="PN977_003036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001399683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HDI6043048.1"
                     /translation="MAKAPKRAFVCNECGADYPRWQGQCSACHAWNTITEVRLAASPT
                     VARNERLSGYAGSAGVAKVQKLSDISLEELPRFSTGFKEFDRVLGGGVVPGSAILIGG
                     NPGAGKSTLLLQTLCKLAQQMKTLYVTGEESLQQVAMRAHRLGLPTDNLNMLSETSIE
                     QICLIAEEEQPKLMVIDSIQVMHMADVQSSPGSVAQVRETAAYLTRFAKTRGVAIVMV
                     GHVTKDGSLAGPKVLEHCIDCSVLLDGDADSRFRTLRSHKNRFGAVNELGVFAMTEQG
                     LREVSNPSAIFLSRGDEVTSGSSVMVVWEGTRPLLVEIQALVDHSMMANPRRVAVGLE
                     QNRLAILLAVLHRHGGLQMADQDVFVNVVGGVKVTETSADLALLLAMVSSLRDRPLPQ
                     DLVVFGEVGLAGEIRPVPSGQERISEAAKHGFRRAIVPAANVPKKAPEGMQIFGVKKL
                     SDALSVFDDL"
     gene            complement(6886..7854)
                     /gene="serB"
                     /locus_tag="PN977_003037"
     CDS             complement(6886..7854)
                     /gene="serB"
                     /locus_tag="PN977_003037"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase"
                     /protein_id="HDI6043049.1"
                     /translation="MPNITWCDLPEDVSLWPGLPLSLSGDEVMPLDYHAGRSGWLLYG
                     RGLDKQRLTQYQSKLGAAMVIVAAWCVEDYQVIRLAGSLTARATRLAHEAQLDVAPLG
                     KIPHLRSPGLLVMDMDSTAIQIECIDEIAKLAGTGEMVAEVTERAMRGELDFTASLRS
                     RVATLKGADANILQQVRENLPLMPGLTQLVLKLETLGWKVAIASGGFTFFAEYLRDKL
                     RLTAVVANELEIMDGKFTGNVIGDIVDAQYKAKTLTRLAQEYEIPLAQTVAIGDGAND
                     LPMIKAAGLGIAYHAKPKVNEKTEVTIRHADLMGVFCILSGSLNQK"
     gene            7960..8604
                     /locus_tag="PN977_003038"
     CDS             7960..8604
                     /locus_tag="PN977_003038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtjB family periplasmic protein"
                     /protein_id="HDI6043050.1"
                     /translation="MARTKLKFRLHRAVIVLFCLALLVALMQGASWFSQNHQRQRNPQ
                     LEELARTLARQVTLNVAPLMRTDSPDEKRIQAILDQLTDESRILDAGVYDEQGDLIAR
                     SGESVEVRDRLALDGKKAGGYFNQQIVEPIAGKNGPLGYLRLTLDTHTLATEAQQVDN
                     TTNILRLMLLLSLAIGVVLTRTLLQGKRTRWQQSPFLLTASKPVPEEEESEKKE"
     gene            8632..9648
                     /gene="lplA"
                     /locus_tag="PN977_003039"
     CDS             8632..9648
                     /gene="lplA"
                     /locus_tag="PN977_003039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase LplA"
                     /protein_id="HDI6043051.1"
                     /translation="MSTLRLLISDSYDPWFNLAVEECIFRQMPATQRVLFLWRNADTV
                     VIGRAQNPWKECNTRRMEEDNVRLARRSSGGGAVFHDLGNTCFTFMAGKPEYDKTIST
                     SIVLNALNALGVSAEASGRNDLVVKTAEGDRKVSGSAYRETKDRGFHHGTLLLNADLS
                     RLANYLNPDKKKLAAKGITSVRSRVTNLTELLPGITHEQVCEAITEAFFAHYGERVEA
                     EIISPDKTPDLPNFAETFARQSSWEWNFGQAPAFSHLLDERFTWGGVELHFDVEKGHI
                     TRAQVFTDSLNPAPLEALAGRLQGCLYRADMLQQECEALLVDFPEQEKELRELSTWIA
                     GAVR"
     gene            9680..9943
                     /locus_tag="PN977_003040"
     CDS             9680..9943
                     /locus_tag="PN977_003040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDI6043052.1"
                     /translation="MIDLENQEREIINLMFSQGISWLTAVRIRHKLSLAEVSKMLGIS
                     INSLKQIEKTERLSSNIKSKMAGIYGCPPELLICPSWMTAEHK"
     gene            complement(10104..10823)
                     /gene="deoD"
                     /locus_tag="PN977_003041"
     CDS             complement(10104..10823)
                     /gene="deoD"
                     /locus_tag="PN977_003041"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HDI6043053.1"
                     /translation="MATPHINAEMGDFADVVLMPGDPLRAKYIAETFLEDAREVNNVR
                     GMLGFTGTYKGRKISVMGHGMGIPSCSIYTKELITDFGVKKIIRVGSCGAVLPHVKLR
                     DVVIGMGACTDSKVNRIRFKDHDFAAIADFDMVRNAVDAAKALGIDARVGNLFSADLF
                     YSPDGEMFDVMEKYGILGVEMEAAGIYGVAAEFGAKALTICTVSDHIRTHEQTTAAER
                     QTTFNDMIKIALESVLLGDKE"
     gene            complement(10880..12103)
                     /gene="deoB"
                     /locus_tag="PN977_003042"
     CDS             complement(10880..12103)
                     /gene="deoB"
                     /locus_tag="PN977_003042"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HDI6043054.1"
                     /translation="MKRAFIMVLDSFGIGATEDAERFGDVGADTLGHIAEACAKGEAD
                     NGRKGPLNLPNLTRLGLAKAHEGSTGFIPAGMDGNAEVIGAYAWAHEMSSGKDTPSGH
                     WEIAGVPVLFEWGYFSDHENSFPQELLDKLVERANLPGYLGNCHSSGTVILDQLGEEH
                     MKTGKPIFYTSADSVFQIACHEETFGLDKLYELCEIAREELTNGGYNIGRVIARPFIG
                     DKAGNFQRTGNRHDLAVEPPAPTVLQKLVDEKHGQVVSVGKIADIYANCGITKKVKAT
                     GLDALFDATIKEMKEAGDNTIVFTNFVDFDSSWGHRRDVAGYAAGLELFDRRLPELMS
                     LLRDDDILILTADHGCDPTWTGTDHTREHIPVLVYGPKVKPGSLGHRETFADIGQTLA
                     KYFGTSDMEYGKAMF"
     gene            complement(12155..13477)
                     /gene="deoA"
                     /locus_tag="PN977_003043"
     CDS             complement(12155..13477)
                     /gene="deoA"
                     /locus_tag="PN977_003043"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313368.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HDI6043055.1"
                     /translation="MFLAQEIIRKKRDGHALSDEEIRFFINGIRDNTISEGQIAALAM
                     TIFFHDMTMPERVSLTMAMRDSGTVLDWKSLHLNGPIVDKHSTGGVGDVTSLMLGPMV
                     AACGGYIPMISGRGLGHTGGTLDKLESIPGFDIFPDDNRFREIIKDVGVAIIGQTSSL
                     APADKRFYATRDITATVDSIPLITASILAKKLAEGLDALVMDVKVGSGAFMPTYELSE
                     ALAEAIVGVANGAGVRTTALLTDMNQVLASSAGNAVEVREAVQFLTGEYRNPRLFDVT
                     MALCVEMLISGKLAKDDAEARAKLQAVLDNGKAAEVFGRMVAAQKGPTDFVENYAKYL
                     PTAMLTKAVYADTEGFVSEMDTRALGMAVVAMGGGRRQASDTIDYSVGFTDMARLGDQ
                     VDGQRPLAVIHAKDENSWQEAAKAVKAAIKLADKAPESTPTVYRRISE"
     gene            complement(13555..14334)
                     /gene="deoC"
                     /locus_tag="PN977_003044"
     CDS             complement(13555..14334)
                     /gene="deoC"
                     /locus_tag="PN977_003044"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005097947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HDI6043056.1"
                     /translation="MTDLKASSLRALKLMDLTTLNDDDTDEKVIALCHQAKTPVGNTA
                     AICIYPRFIPIARKTLKEQGTPEIRIATVTNFPHGNDDIEIALAETRAAIAYGADEVD
                     VVFPYRALMAGNEQVGFDLVKACKEACAAANVLLKVIIETGELKDEALIRKASEISIK
                     AGADFIKTSTGKVAVNATPESARIMMEVIRDMGVEKTVGFKPAGGVRTAEDAQKYLSI
                     ADELFGADWADARHYRFGASSLLASLLKALGHGDGKSASSY"
     gene            14592..16142
                     /locus_tag="PN977_003045"
     CDS             14592..16142
                     /locus_tag="PN977_003045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjI family glycine radical enzyme"
                     /protein_id="HDI6043057.1"
                     /translation="MPTSHENALQQRCQQIVTSPVLSPEQKRHFLALEAENNLPYPAL
                     PAEARRALDEGVICDMFEGHAPYKPRYVLPDYARFLANGSEWLELEGAKDLDDALSLL
                     TILYHHVPSVTSMPVYLGQLDALLQPYVRILTQDEIDIRIKRFWRYLDRTLPDAFMHA
                     NIGPSDSPITRAILRADAELKQVSPNLTFIYDPEITPDDLLLEVAKNICECSKPHIAN
                     GPVHDKIFTKGGYGIVSCYNSLPLAGGGSTLVRLNLKAIAERSESLEDFFTRTLPHYC
                     QQQIAIIDARCEFLYQQSHFFENSFLVKEGLINPERFVPMFGMYGLAEAVNLLCEKEG
                     IVARYGKEATANEVGYRISAQLAEFVANTPVKYGWQKRAMLHAQSGISSDIGTTPGAR
                     LPYGDEPDPITHLQTVAPHHAYYYSGISDILTLDETIKRNPQALVQLCLGAFKAGMRE
                     FTANVSGNDLVRVTGYMVRLSDLEKYRAEGSRTNTTWLGEEAARNTRILERQPRVISH
                     EQQMRFSQ"
     gene            16114..16977
                     /locus_tag="PN977_003046"
     CDS             16114..16977
                     /locus_tag="PN977_003046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjW family glycine radical enzyme activase"
                     /protein_id="HDI6043058.1"
                     /translation="MNSRCALVSKIIPFSCVDGPGSRLALFLQGCNLRCKNCHNPWTM
                     GRCNDCGECVPQCPHQALQIIEGKVAWSAAVCEQCDTCLKMCPQHATPMAQSMSVDEV
                     LNHVRKAVLFIEGITVSGGEATTQLPFVVALFTAIKNDPQLCHLTCLVDSNGMLSETG
                     WEKLLPVCDGAMLDLKAWGSECHQQLTGRDNQQIKRSICLLAERGKLAELRLLVIPGQ
                     VDYLQHIEELAALIKGLGDVPVRLNAFHAHGVYGEAQSWPSATPEDVEQLADALRERG
                     VSRLIFPALYL"
     gene            complement(17007..17789)
                     /locus_tag="PN977_003047"
     CDS             complement(17007..17789)
                     /locus_tag="PN977_003047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HDI6043059.1"
                     /translation="MICRFIDTHCHFDFPPFSGDEEASLQRAAQAGVGKIIVPATEAE
                     NFARVQALAEKYQPLYAALGLHPGMLEKHSDVSLDQLQQALERHPAKVVAVGEIGLDL
                     FGDDPQFERQQWLLDEQLKLAKRYDLPVILHSRRTHDKLAMHLKRHDLSRAGVVHGFS
                     GSQQQAERFVQLGYKIGVGGTITYPRASKTRDVIAKLPLASLLLETDAPDMPLNGFQG
                     QPNRPEQAARVFAVLCELRPEPADEIAEVLLNNTNEVFNMRG"
     gene            complement(17786..18859)
                     /locus_tag="PN977_003048"
     CDS             complement(17786..18859)
                     /locus_tag="PN977_003048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin family protein"
                     /protein_id="HDI6043060.1"
                     /translation="MGQRIPVTLGNIAPLSLRPFQPGRIALVCEGGGQRGIFTAGVLD
                     EFMRAQFNPFDLYLGTSAGAQNLSAYICNQPGYARKVIMRYTTKREFFDPLRFVRGGN
                     LIDLDWLVEATASQMPLQMDTAARLFDSGKSFYMCACRQDDYAPNYFLPTKQNWLDVI
                     RASSAIPGFYRSGVSLEGINYLDGGISDAIPVKEAARQGAKTLVVIRTVPSQMYYTPQ
                     WFKRMERWLGDSSLQPLVNLVQHHETSYREIQQFIEKPPGQLRIFEIYPPKPLHSIAL
                     GSRIPALREDYKLGRLCGRYFLATVGKLLTEKAPLSRHLVPVVTPESIVIPPAPVAND
                     TLVAEVSDAPQANDPTFNNEDLA"
     gene            complement(18981..19142)
                     /locus_tag="PN977_003049"
     CDS             complement(18981..19142)
                     /locus_tag="PN977_003049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002887716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 domain-containing protein"
                     /protein_id="HDI6043061.1"
                     /translation="MFRWGIIFLVIALIAAALGFGGLAGTAAGAAKIVFVVGIILFLV
                     SLFMGRKRP"
     gene            complement(19269..19874)
                     /gene="osmY"
                     /locus_tag="PN977_003050"
     CDS             complement(19269..19874)
                     /gene="osmY"
                     /locus_tag="PN977_003050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone OsmY"
                     /protein_id="HDI6043062.1"
                     /translation="MTMTRLKISKTLLAVMLTSAVATGSAYAENNAQTTNESAGQKVD
                     SSMNKVGNFMDDSAITAKVKAALVDHDNIKSTDISVKTDQKVVTLSGFVESQAQAEEA
                     VKVAKGVEGVTSVSDKLHVRDAKESSVKGYAGDTATTSEIKAKLLADDIVPSRHVKVE
                     TTDGVVQLSGTVDSQAQSDRAESIAKAVDGVKSVKNDLKTK"
     gene            complement(20267..21856)
                     /gene="prfC"
                     /locus_tag="PN977_003051"
     CDS             complement(20267..21856)
                     /gene="prfC"
                     /locus_tag="PN977_003051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HDI6043063.1"
                     /translation="MTLSPYLQEVAKRRTFAIISHPDAGKTTITEKVLLFGQAIQTAG
                     TVKGRGSNQHAKSDWMEMEKQRGISITTSVMQFPYHDCLVNLLDTPGHEDFSEDTYRT
                     LTAVDCCLMVIDAAKGVEDRTRKLMEVTRLRDTPILTFMNKLDRDIRDPMELLDEVEN
                     ELKIGCAPITWPIGCGKLFKGVYHLYKDETYLYQSGKGHTIQEVRIVKGLNNPDLDAA
                     VGEDLAQQLRDELELVKGASNEFDKELFLAGEITPVFFGTALGNFGVDHMLDGLVEWA
                     PAPMPRQTDTRTVEASEDKFTGFVFKIQANMDPKHRDRVAFMRVVSGKYEKGMKLRQV
                     RTAKDVVISDALTFMAGDRSHVEEAYPGDILGLHNHGTIQIGDTFTQGEMMKFTGIPN
                     FAPELFRRIRLKDPLKQKQLLKGLVQLSEEGAVQVFRPISNNDLIVGAVGVLQFDVVV
                     ARLKSEYNVEAVYESVNVATARWVECADAKKFEEFKRKNESQLALDGGDNLAYIATSM
                     VNLRLAQERYPDVQFHQTREH"
     gene            complement(21947..22624)
                     /gene="yjjG"
                     /locus_tag="PN977_003052"
     CDS             complement(21947..22624)
                     /gene="yjjG"
                     /locus_tag="PN977_003052"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine 5'-nucleotidase"
                     /protein_id="HDI6043064.1"
                     /translation="MKWDWIFFDADETLFTFDSFTGLQRMFLDYSVTFTAEDFQDYQA
                     VNKPLWVDYQNGAITSLQLQHGRFESWAERLNVEPGKLNEAFINAMAEICTPLPGAVS
                     LLNAIRGNAKIGIITNGFSALQQVRLERTGLRDYFDLLVISEEVGVAKPNKKIFDYAL
                     EQAGNPDRSRVLMVGDTAESDILGGINAGLATCWLNAHNREQPEGIAPTWTVSSLHEL
                     EQLLCKH"
     gene            complement(22639..23085)
                     /gene="rimI"
                     /locus_tag="PN977_003053"
     CDS             complement(22639..23085)
                     /gene="rimI"
                     /locus_tag="PN977_003053"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HDI6043065.1"
                     /translation="MNTISSLETTDLPAAYHIEQRAHAFPWSEKTFASNQGERYLNFQ
                     LTQNGKMAAFAITQVVLDEATLFNIAVDPDYQRQGLGRALLEHLIDELEKRGVATLWL
                     EVRASNAAAIALYESLGFNEATIRRNYYPTTDGREDAIIMALPISM"
     gene            complement(23054..23467)
                     /gene="holD"
                     /locus_tag="PN977_003054"
     CDS             complement(23054..23467)
                     /gene="holD"
                     /locus_tag="PN977_003054"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit psi"
                     /protein_id="HDI6043066.1"
                     /translation="MTSRRDWQLQQLGITQWSLRRPGALQGEIAIAIPAHVRLVMVAN
                     DLPALTDPLVSDVLRALTVSPDQVLQLTPEKIAMLPQGSRCNSWRLGTDEPLSLEGAQ
                     VASPALTELRANPTARAALWQQICAYEHDFFPRND"
     gene            23570..>23910
                     /gene="rsmC"
                     /locus_tag="PN977_003055"
     CDS             23570..>23910
                     /gene="rsmC"
                     /locus_tag="PN977_003055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418788.1"
                     /note="16S rRNA M2G1207 methyltransferase; one of many
                     enzymes that modify bases of the ribosomal RNA; this
                     enzyme methylates the G at position 1207 of the small
                     ribosomal subunit; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1207)-N(2))-methyltransferase
                     RsmC"
                     /protein_id="HDI6043067.1"
                     /translation="MSAFTPASEVLLRHSDDFEQSRILFAGDLQDDLPARLDTAASRA
                     HTQQFHHWQVLSRQMGDNARFSLVATANDVADCDTLIYYWPKNKPEAQFQLMNLLSLL
                     PVGTDIFVVGE"
BASE COUNT         5582 a         6584 c         6164 g         5580 t
ORIGIN      
        1 attgttagcg ggagaagcaa aatgcctccc ccggttgtac tcgctaaaga gtacgatagc
       61 atatcataaa cgtgtggatc agtaacgacg tccccattcc gtggcgctca tcaacgtcgg
      121 tttatccccc atgaaatagc ggtagtaagc gtcataagcc agcacgtttt tcacataacc
      181 gcgcgtctcg gagaatggaa tactctcgac aaatgccact gcgtcgatac gccctgcgct
      241 gttgccaagc caggttcgca cccgccctgg tccggcgtta taagctgctg aggagaaaat
      301 acggttattg ccaaactgct gataaacata ttgcaggtaa ctggtgccaa tgttgatgtt
      361 tgtttccgga tccagcaatt gcccagggct gctataaccg ggaatagaga acatcttcac
      421 cgtatgggtc gctgtacccg gcataatctg catcaggccg ctggctccta ccggcgattt
      481 cactttcgga ttccaggcac tctcctgacg ggcaatcgcc atcgcatagc tttgcgggat
      541 ctccttccca ctggtgtaac gtttgaaaag atcgttgtaa gccagcggga atctctcttc
      601 cagatgatcc cacagcttcc cggcgatcgt tgcctgaacg ctgagatccc accattggtt
      661 attgaaagca taccgcgcca gttgcgcctg ctctgttttt gacttgctct tcaccagatt
      721 ggcccactcg ctacgcgcgg tgttatcgag attccagtac atcaactcgc gcacgcgcgc
      781 catctccggc ccctgagtca gggcgctgtc aacattctgc ggcgctttat caatcttcag
      841 ctcatactct tcgccgatgc gttgtgcagc aaccatcggg tagaaaccac gctgttgcat
      901 gagttgatgc aaaatctctt ttgcttcagc ttcacgtccg cgttcaagca gtaaatccgc
      961 ctgccagtaa cgccattcat ctttctcttt cgcctccatc ggcagacgcg ccagccaggt
     1021 attcaggccg cgacgatcgc cggtgccaag cgccattcgc acacggcgtt caataagcga
     1081 agtagattgc gaacgcataa tggcgtcatc acgccatttc gcctgttcgt cggtgacatc
     1141 gttgcccatc aaacgccagg cgacgatatc gcgcagttcc tgaatctgat cttcattaag
     1201 ctgctgtgcc tgagcaagcg atggaatcat cagccgtgca ttttcagcat cctgccgcgc
     1261 cacgctggca aacgccaccg ccgccatttg acgggtaaaa tcggtcgcgc cggttgtacg
     1321 cgcgaaggtc agtaccgtat tagggttgtt cgccagtgaa atgattgccg aagcgatagt
     1381 ctggtaatcg gcaggcatct gccctgccag cactgttacc aggccggtgt tacccgcttt
     1441 catcgccaga cggatacgct ctaaatacgc cagcggatct tgtttacctg acgcacgcca
     1501 gacgctaaat aacttgtcac aggcgttagg ctggctcttg ccggttagcc acagctcttt
     1561 cgccccttgc caggcttctt cactctgccc ggtgttccat ttcgcatagt agtaattaca
     1621 ttgcgcttcg gtagttccgg gtttttccgg gctaaaggct aacaagccac gccagtcttc
     1681 acgccgcgcc agttcattga cgaaacgaga ttgcagcgtg cgagcgggag gaagcgtggg
     1741 gttagcgcga acaaagttag tgaccgtcac cgccggttga ttcatcaggt catcggtgat
     1801 ctggcggtat tccagatagg gataaagtgg ataatccttc aatccaggca tcatttgttc
     1861 gaccacatcc atttgtcgat tatcccaggc ctgcttaatt tgcgcgtaac ggctacgctg
     1921 ctcatccagt gagtcggctc gcgccacgct gctgaccgtc agcagacaga caccgacagc
     1981 caacagccgc caggtaacgt gtttggcttt ttccacaagc gcatcctcta agtgtcaata
     2041 catcaatgca gcgtcatgcc gcgtaataga tttatgctaa ccagtcattg ccgtttacgc
     2101 cacgttacgg acactttttt acttttactg cgagggtgat cgaccggctg ggattagata
     2161 aggaaaaagg ctacacttcg cctttgaaaa ctcatcatca cgataaacat agaggcgaag
     2221 tccaacgtgg ctcaattcgt ttataccatg catcgtgtcg gcaaagttgt tccgccgaaa
     2281 cgtcatattt tgaaaaacat ctctctgagt ttcttccctg gggcaaaaat tggtgtcctg
     2341 ggtctgaacg gcgcgggtaa gtctaccctg ctgcgcatta tggcgggcat tgataaagat
     2401 atcgaaggtg aagcgcgtcc gcagccagac atcaagattg gttacctgcc gcaggaacct
     2461 cagctgaacc cggaacacac cgtgcgtgag tccattgaag aagcggtttc tgaagtggtt
     2521 aacgctctga aacgtctgga tgaagtgtat gcgctgtacg ccgatccgga tgccgatttt
     2581 gacaagctgg ccgctgaaca aggccgtctg gaagagatca ttcaggctca tgacggtcat
     2641 aacctgaacg ttcagctgga gcgtgcggct gatgcgctgc gtctgccgga ctgggacgcg
     2701 aaaatcgcta acctttccgg tggtgagcgt cgtcgcgtgg cgttgtgccg cctgctgttg
     2761 gaaaaaccag acatgctgct gctcgacgaa ccgaccaacc acctggatgc cgaatccgtg
     2821 gcctggctgg aacgcttcct gcacgacttc gagggtaccg tggtggcgat tacccacgac
     2881 cgttacttcc tcgataacgt tgcaggctgg atcctcgaac ttgaccgcgg tgaaggtatt
     2941 ccgtgggaag gcaactactc ctcctggctg gagcagaaag atcagcgcct ggcgcaggaa
     3001 gcttcacaag aagcggcgcg tcgtaagtcg attgagaaag agctggagtg ggtacgtcag
     3061 ggaactaaag gccgccagtc gaaaggtaaa gcccgtctgg cgcgctttga agagctgaac
     3121 agcaccgaat atcagaaacg taacgaaacc aacgaactgt ttattccacc tggaccacgt
     3181 ctgggcgaca aagtgctgga agtcagcaat ctgcgtaaat cctatggcga tcgtctgctg
     3241 attgactccc tgagtttctc gatcccgaaa ggggcgatcg tcgggatcat cggtccgaac
     3301 ggtgcgggta aatcgaccct gttccgtatg atctccggtc aggaacagcc ggacagcggc
     3361 accatcactt tgggtgaaac ggtgaaactg gcatcggttg atcagttccg tgactcaatg
     3421 gataacagca aaaccgtttg ggaagaagtt tccggcgggc tggatatcat gaagatcggc
     3481 aacaccgaga tgccaagccg cgcctacgtt ggccgcttta actttaaagg ggttgatcag
     3541 ggtaaacgcg ttggcgaact ttccggtggt gagcgtggtc gtctgcatct ggcgaagctg
     3601 ctgcaggttg gcggcaacat gctgctgctc gacgaaccga ccaacgacct ggatatcgaa
     3661 accctgcgcg cgctggaaaa cgccctgctg gagttcccgg gctgcgcgat ggttatctcg
     3721 cacgaccgtt ggttcctcga ccgtatcgcc acacacattc tggattacca ggatgaaggt
     3781 aaagttgagt tctttgaagg taactttacc gagtacgaag agtacaagaa acgcacgctg
     3841 ggcgcagacg cgctggagcc gaagcgtatc aagtacaagc gtattgcgaa gtaatgcgta
     3901 aaatgccggg tgcggcgtga acgccttatc cggcctacaa accaagaaat ttcaatacgt
     3961 tgtgaatctc caggcctgat aagacgcggt aagcgtcgca tcaggcattg agcaccgaat
     4021 gcgggatgcg gcgtgaacac cttttccggt ctacaacagc cttagcatcc tcaatttcgt
     4081 ggcgggtcac ccgtgcaggt tttttcaaac cttgtttaat ctcgacagcc tcttccagag
     4141 aggttttcaa ctcatcaaaa aaactcattt tatacctcgc ctttgagcag tttcgtcacg
     4201 gttatccttg ctcccccatc atctcccgca ccagctccac gcagcgcagg aaacggctgt
     4261 cgtaatcgtc ctcttcaacc cgcacgaatt cgatattgtt ctcttccagc atctccacca
     4321 gcaagttctg gaactctttg cgatccaccg aactgccgag gctgcgtaaa ccatccgcca
     4381 cccacggcgt gttgttctcc agcaggatca ccagatcgaa acggtattca tcaatcagcg
     4441 cctgcacgaa cggatgctca cgcccttcgt actttttgca gaacgcctgg gtagtgacaa
     4501 aatcggtgtc aataaacgcc actttattgg cgtatttcac tgcaaaatca atgtattgtg
     4561 cgtggcccaa cgcgatttta tcgtagtcgg aatactgcaa tgcgatctca tcaccgccga
     4621 ggtgtgaaaa gacataatcg cggccatatt cccacgcact ggtggtgttg aagatattgg
     4681 caagtttgtt taccagggtg gatttaccgc tcgactcgcc gccaaggatc gccacggtac
     4741 gcacaaagaa tggcttcact tccgtaggaa tatattccca gtagcggaac gggttttcgc
     4801 ggatctgcgc accgctgata ctcataaagg tgcgtttcgg atcgaccagc accgtttcga
     4861 tccccagatg ttccatatac tgtggcgcat cggcttcttc cgaggtgtag atcaggtccg
     4921 gctggatccc cttttcagcc ataaactttt tgatgccgtt gctccacaca tcccagccgt
     4981 gtggatacgg ctccatgccc tcttcgttga aggcatgaat gcgaatattt ttctgatatt
     5041 taaaggtttg taataaccag cgcaaacgat ccggcaccgt gggctgctgc gacatggcac
     5101 tgtcttcaaa caacgcacgg tcacgggtat cgtcaaagcc cataatgata tgcagttcgt
     5161 caacctggct acaggcgcgc tggataaggt agatatgtcc ggtatgcaac gggtagaact
     5221 taccgaatac cacgccaatg gttttctttt ggcggggaaa ctcaagcccc aaaaaacggt
     5281 gcaacgcctc cagtttttgc gcgctggggc ttttaatttt ggcattcagt aactggctta
     5341 aatacccttt ggtcataccg ctggcatcag ccacctgctg tagcgtacag ccctgttgct
     5401 taatggcagt tttcaggtaa tcaaatgacg acatatctcc ctccgtatct ctcattataa
     5461 gtcgtcgaac acgctaagcg cgtcggagag ttttttaacg ccaaaaatct gcatcccttc
     5521 cggcgctttt ttcggcacgt tagccgccgg aacaatcgcc cgccgaaaac catgtttcgc
     5581 cgcttctgag atacgttcct gaccgctggg caccgggcgg atctcccctg ccagcccgac
     5641 ttcaccaaac accaccagat cctgcggcag cggtctgtcg cgcaggctgg aaaccatcgc
     5701 cagcagtaac gctaagtcgg cgctggtttc cgtcaccttc acgccaccga ccacgttaac
     5761 aaacacatcc tgatcggcca tttgtagacc accgtgacgg tgaagcaccg ccagcaggat
     5821 tgccagacgg ttttgctcca gcccgactgc cacgcggcgt ggattcgcca tcatcgagtg
     5881 atcgacgagc gcctgaatct ccaccagcag cggacgcgtt ccttcccaca ctaccatcac
     5941 tgagctaccc gaggtcactt catcaccgcg gcttaaaaag attgccgaag ggttgctgac
     6001 ttcacgcaga ccctgttcgg tcattgcgaa gacgcccagc tcattcaccg cgccgaagcg
     6061 gtttttatgg ctgcgtaagg tgcgaaaacg ggagtcggca tcgccatcca gaagcaccga
     6121 acagtcgata cagtgttcca gcactttagg gccagccagc gagccatctt tggttacgtg
     6181 cccaaccatg acaatcgcca caccgcgcgt tttggcgaag cgcgtcagat aagccgccgt
     6241 ttcacgcacc tgcgccacgc tgccaggcga tgattgtaca tccgccatat gcatcacctg
     6301 gatggagtca attaccatca gcttcggttg ctcttcttcg gcaatcaggc agatctgctc
     6361 gatgctggtt tccgacaaca tattgagatt gtcagtcggc aggccaaggc gatgagcgcg
     6421 cattgccacc tgttgcagcg actcttcgcc ggtgacatac agcgttttca tctgctgggc
     6481 cagtttgcac agcgtttgca acagcagcgt ggatttcccc gcacctgggt taccgccaat
     6541 cagaatggcg cttcccggca ccaccccgcc gcctaatacg cggtcgaact ctttaaatcc
     6601 ggtagaaaaa cgcggcagct cctcaaggct gatgtcggag agtttctgga cttttgccac
     6661 tccggcgcta ccggcatagc cactgagacg ctcgttacgc gccaccgttg gcgacgcagc
     6721 aagacgcacc tcggtgatgg tgttccaggc atgacaggca ctgcactgcc cctgccagcg
     6781 cggataatcg gccccgcatt cattacaaac aaaggcgcgt tttggagctt ttgccacggt
     6841 gtacctcgtt aatgctgtgc cgcccgcagg atgacgggca ggcaattact tctgattcag
     6901 gctgccagag aggatgcaga atacccccat caggtcagcg tgacggatgg tgacttccgt
     6961 tttttcattc acttttggct tggcatggta ggcaatcccc agccctgccg ctttgatcat
     7021 cggcaggtca ttggctccat cgccaatcgc cacggtctgc gccagcggga tttcatactc
     7081 ctgcgcgagg cgagtcagag ttttcgcttt gtactgcgcg tctacgatgt cgccgatcac
     7141 attgccggta aatttaccgt ccatgatctc cagttcattg gctaccacgg ctgtcaggcg
     7201 cagcttgtcg cgcaggtatt cagcaaagaa agtaaagccg ccggaagcaa tcgccacttt
     7261 ccagcccagc gtttccagct tgagcaccag ttgcgttaag cctggcatca gcggcagatt
     7321 ttcacgcacc tgttgcagga tattggcgtc agcgcctttc agcgtcgcca cacggctgcg
     7381 caggctggcg gtaaaatcga gttcgccgcg catcgcccgt tcggttacct ccgccaccat
     7441 ctcgccggtt ccggccagtt tggcaatttc gtcaatacat tcaatctgaa tggcggtgga
     7501 atccatgtcc atcaccagca aacccggaga gcgcaggtgc gggattttcc ccaacggcgc
     7561 gacatccagt tgcgcttcgt gggccaggcg cgtagcccgc gcggtgagtg aacctgccag
     7621 acgaatcacc tgataatctt ccacgcacca ggcggcaaca atcaccatag ccgcgcccag
     7681 tttgctctgg tattgggtca gacgttgttt atccagccca cgaccataca gcagccagcc
     7741 gctacgacct gcgtggtaat ccagtggcat cacttcatca ccacttaatg aaagaggcag
     7801 accaggccat aaagagacat cttcaggcag gtcgcaccag gtaatgttag gcattaaggc
     7861 tcctgtaaaa tcgttcgaag cagggaaaat aacgcatgag gctaccttgt atccattgct
     7921 tctggcaaca ttaagtctca aattttcaaa gggtggaaga tggctcgcac aaaactgaaa
     7981 ttccggctgc atcgggcagt gattgtcctg ttctgtctcg ccttgttagt ggcgctgatg
     8041 cagggggcgt catggtttag tcaaaaccac cagcgacagc gtaatccaca gctggaagaa
     8101 ctggcccgca ccctggcgcg tcaggtaacg ctgaatgttg caccgctgat gcgtaccgac
     8161 tcaccggatg aaaaacgtat tcaggcgatc cttgatcagt taacggatga aagccgcatc
     8221 ctcgacgcgg gcgtgtatga cgaacaaggc gatcttatcg cccgttctgg cgaaagcgtc
     8281 gaagtgcgcg accggttggc gctcgacggt aaaaaagcgg gcggctattt taaccagcag
     8341 attgtcgaac ccattgcggg taaaaacgga ccgctcggct atctgcgcct gacgctcgac
     8401 acccatacgc tcgccaccga agcccaacag gtggataaca ccactaacat tttacgcctg
     8461 atgttgctgc tctcgctggc aatcggcgta gtgctgaccc gtacgctgct acagggtaaa
     8521 cgcacccgct ggcagcaatc gcccttcctg ttaaccgcca gcaaaccggt gccggaagag
     8581 gaagaaagcg agaaaaaaga gtgacccatt actacaagaa aggaaatcgt tatgtccaca
     8641 ttacgcctgc tcatctctga ctcttacgac ccgtggttta acctggcggt ggaagagtgt
     8701 atttttcgcc agatgcccgc cacgcagcgc gttctgtttc tctggcgcaa tgccgacacc
     8761 gtggtgattg gtcgcgcaca gaacccgtgg aaagagtgta atacccggcg gatggaagaa
     8821 gataacgtcc gcctggcgcg gcgcagcagc ggtggcggcg cggtgttcca cgatctcggt
     8881 aatacctgct ttacctttat ggctggcaag ccggagtacg ataaaactat ctccacgtcg
     8941 attgtgctca atgctctgaa cgcgctcggc gtcagcgccg aagcgtccgg ccgtaacgat
     9001 ctggtggtga aaaccgccga aggcgaccgc aaagtctcag gctcagccta tcgcgaaact
     9061 aaagatcgcg gcttccatca tggcaccttg ctactcaatg ctgaccttag ccgtctggca
     9121 aactatctca acccggataa aaagaaactg gcggcgaaag gcattacgtc ggtacgttcc
     9181 cgcgtgacca acctcaccga gctgctgccg gggatcaccc atgagcaggt ttgcgaggcc
     9241 ataaccgagg cctttttcgc ccattatggc gagcgcgtgg aagcggaaat catctccccg
     9301 gacaaaacgc cagacttgcc gaacttcgcc gaaacctttg cccgtcagag tagctgggag
     9361 tggaacttcg gtcaggctcc ggcgttctcg catctgctgg atgaacgctt cacctggggc
     9421 ggcgtggaac tgcatttcga cgttgaaaaa ggccatatca cccgcgccca ggtgtttacc
     9481 gacagcctca accccgcacc tctggaagct ctcgccgggc gactgcaagg ctgcctgtac
     9541 cgcgcggata tgctgcaaca ggagtgcgaa gcgctgttgg ttgacttccc ggaacaggaa
     9601 aaagagctac gggagttgtc gacgtggata gcgggggcgg taaggtaact cactgattta
     9661 aatatccagg gaaggaaata tgatcgatct ggaaaaccag gaacgggaga ttatcaattt
     9721 aatgttcagc cagggtatat cctggctaac cgccgtgcgt atcaggcata aactatcact
     9781 tgctgaagta agtaaaatgt taggtatctc cattaattct ctaaaacaga tcgaaaagac
     9841 ggaacggctt agttcaaaca tcaaaagcaa aatggcagga atttatggtt gtccaccaga
     9901 gttgctgata tgcccttcct ggatgacggc tgaacataaa tgaatagatt cgtgaataga
     9961 tacttgaatg gattttattt tctatccata tgaaaaataa tagtaaatct ataaatcagt
    10021 tgctattgat gaatagatac taaaaagccg gagcagtctc ccgctccggc ttcacaaggc
    10081 aatcgccttt cagcgaaaca caattactct ttatcgccca acagaacgga ttccagtgcg
    10141 attttgatca tgtcgttgaa ggtggtctga cgctcagcgg cagtggtctg ctcgtgagtg
    10201 cggatgtggt cagacacggt gcagatggtc agggctttcg cgccaaattc tgcagcgacg
    10261 ccgtagatac cagctgcttc catttccacg ccgaggatgc cgtatttttc catcacgtcg
    10321 aacatttcgc cgtccggaga gtaaaacagg tcagcggaga acaggttacc aacgcgagca
    10381 tcaatgccca gtgctttagc tgcatctacc gcgttacgca ccatgtcaaa gtcagcgata
    10441 gcggcaaagt catggtcttt aaaacggata cggttaactt tggaatcggt gcaggcaccc
    10501 ataccgataa cgacgtcgcg cagttttacg tgcggcagaa cagcgccaca ggagcctacg
    10561 cggataattt tcttcacgcc gaaatcggtg atcagttctt tggtgtagat ggagcaggac
    10621 gggataccca taccgtgccc cattacggaa attttgcggc ctttgtaagt accggtgaag
    10681 cccagcatac cgcgaacgtt gttcacttca cgggcatctt caaggaaagt ttcagcaata
    10741 tacttcgcac gcagcgggtc gcctggcatc aaaactacgt cagcgaaatc gcccatttct
    10801 gcattaatgt gtggggtagc cattgtttta tcctttttgt gacataacaa aggcggagtc
    10861 aacgctccgc ccaaatccat cagaacatgg ctttgccata ttccatatca gaagtaccaa
    10921 aatattttgc cagcgtctgg ccgatatccg cgaaggtttc acggtgaccc agtgagcccg
    10981 gttttacttt cgggccgtat accagtaccg gaatgtgttc acgcgtgtgg tcagtaccgg
    11041 tccaggtcgg atcacaaccg tggtcagcgg tgaggatcag gatgtcgtca tcgcgcagca
    11101 gagacatcag ctccggcaga cgacggtcga acagctccag acccgcagca taaccggcaa
    11161 cgtcgcgacg gtggccccag gaagagtcga agtcaacgaa gttggtgaag acgatagtgt
    11221 tatcacccgc ttctttcatc tctttgatgg tggcgtcaaa cagcgcgtcc aggccagtcg
    11281 ctttcacttt cttggtgatg ccgcagttag cgtagatgtc cgcaatttta ccgacagaaa
    11341 ccacctggcc gtgtttttca tcaaccagtt tctgcagcac ggtcggtgct ggcggttcaa
    11401 cagccagatc gtgacggtta ccggtacgct ggaagttacc ggctttgtcg ccgataaacg
    11461 gacgagcgat aacacgaccg atgttgtagc cgccgttggt cagctcttca cgagcgattt
    11521 cgcacagttc gtagagtttg tccagaccga aagtttcttc atggcaggca atctggaaca
    11581 cggagtcagc ggaggtatag aaaatcggct tgccggtttt catgtgctct tcgcccagct
    11641 gatccagaat gaccgtaccg gaagagtggc agttaccgag gtaacccggc agattagcgc
    11701 gttcgaccag tttatccagt agctcttgcg ggaagctgtt ttcgtgatcg gagaagtatc
    11761 cccactcaaa cagaaccggg acaccggcga tttcccagtg accagacggg gtatctttac
    11821 cggatgacat ttcgtgcgcc catgcgtacg cgccgataac ttcagcgttg ccgtccattc
    11881 ccgccggaat gaaaccggta gaaccttcgt gtgctttcgc cagccccaga cgggtcagat
    11941 ttggcagatt gagcgggcct ttacgaccgt tatcagcttc gcctttggca caagcttctg
    12001 cgatatgacc cagggtgtca gccccgacgt caccaaagcg ttctgcatct tctgtagcgc
    12061 cgatgccgaa tgagtccagc accataataa atgcacgttt catatgttct ccgtactcag
    12121 tgcttcaaat ttaaatgatc agatcagtat accgttattc actgatacga cgatagacag
    12181 ttggtgtgct ttccggtgct ttatcggcaa gtttaattgc cgctttcacc gctttcgccg
    12241 cttcctgcca gctgttttcg tctttcgcgt ggataactgc cagcggacgc tgaccgtcta
    12301 cctggtcgcc cagacgcgcc atatcagtaa agccgacgct gtaatcgatg gtgtcagatg
    12361 cctgacggcg tccgccgccc attgcaacca ctgccatccc cagcgcgcgg gtatccattt
    12421 cactgacaaa cccttcggta tcagcataga ctgctttcgt cagcatcgct gtcggcagat
    12481 acttcgcgta gttctcaacg aagtcagtcg ggcctttttg tgccgctacc atacgaccaa
    12541 agacttctgc cgctttaccg ttgtccagca ccgcctgcaa tttcgcgcgc gcttcggcgt
    12601 catctttcgc cagtttgccg gagataagca tctctacgca cagcgccatt gtgacatcaa
    12661 acagacgcgg gttacgatac tcacccgtca ggaactgcac cgcttcacga acttcaaccg
    12721 cgttacctgc actggaggcc agtacctgat tcatatcggt gagcagcgcg gtggtacgca
    12781 cgccagcgcc gttagctacg ccaacaatcg cttcggcaag ggcttcagaa agttcgtagg
    12841 tcggcataaa cgcgccgcta cccactttca cgtccatcac cagcgcatcc agaccttccg
    12901 ccagtttctt cgccaggatc gaggcagtga tcagcgggat ggagtccacg gttgcggtaa
    12961 tatcacgggt cgcgtagaaa cgtttatccg ccggagccag tgagctggtc tgaccgataa
    13021 tcgccacgcc gacgtcttta ataatttcgc ggaaacggtt gtcatccggg aaaatgtcga
    13081 agccagggat ggattccagt ttgtcgagcg taccgccagt atgaccgagg ccgcgaccgg
    13141 agatcatcgg aatatagccg ccacaggcag cgaccatcgg ccccaacatc agcgaagtca
    13201 catcgccgac accgccggtc gagtgtttat caacaatcgg gccattcaga tgcaggcttt
    13261 tccagtcgag aacggttcct gaatctcgca tcgccatggt cagcgagaca cgctcaggca
    13321 ttgtcatatc gtggaagaaa atggtcatcg cgagggcggc aatctgccct tcggagatag
    13381 tgttgtcgcg aatgccgttg ataaagaaac gaatttcttc atcactcagc gcatgaccat
    13441 cacgtttttt acgaataatt tcttgtgcga gaaacaaggt aacctcccgt ggaaaagaga
    13501 cgaggtaatc ggggaaccgg atgagcatcg ctgcatcatc cggcaaatca agccttagta
    13561 gctgctggcg ctcttaccgt cgccgtggcc cagagctttc agcaggcttg ccagcaagct
    13621 ggaagcacca aagcggtagt gacgcgcatc agcccagtca gcaccgaaca gttcatcggc
    13681 gatggagaga tatttctgcg catcttccgc agtacgcacg ccgcccgccg gtttgaaacc
    13741 aacggttttt tctacgccca tatcgcggat cacttccatc atgatgcgcg cgctttccgg
    13801 cgtcgcattc acagccactt taccggtaga ggttttgatg aagtcagcac ccgctttgat
    13861 ggagatttca gacgctttac ggatcagcgc ttcgtctttc agttctccgg tttcaataat
    13921 cactttcagc aggacgttcg ccgcagcgca agcctcttta caggctttca ccaggtcaaa
    13981 accaacctgc tcgttacccg ccatcagcgc gcggtacggg aacaccacgt caacttcatc
    14041 ggcaccgtag gcgattgccg cacgggtttc tgccagcgcg atttcgatgt cgtcgttacc
    14101 gtgtgggaag ttagttaccg tagcgatacg gatttccggg gtgccctgct ctttcagagt
    14161 tttgcgagca atcgggataa agcgaggata gatacagata gcggcggtat tgccgaccgg
    14221 agttttggcc tgatgacaca gggcgatcac tttctcatcg gtgtcgtcgt cattcagggt
    14281 ggtcaggtcc atcaatttca gtgcacgcag gctgcttgct ttcagatcag tcatttcatt
    14341 ctcctggctt gtcgccaata aaattcacct tgcgagtttg ttagtattct aacatctact
    14401 ccgcaacaca cttcgatgta catcacaatt aaggaaatct acttacaagt ttgcatcact
    14461 gtaatgcgat ctggttcaaa taattcactt tcaaatgaat gcgtcagtgg tggcaaacgc
    14521 atcaggatca aagtgaacat cacgaaactt cttacaatgg cgcatcacct tcggcataaa
    14581 aggaaaagaa gatgcccact tctcatgaaa atgcactgca acaacgttgc cagcaaattg
    14641 tcaccagccc ggtacttagc ccggagcaga agcgccattt tctggcactg gaagcagaaa
    14701 acaatctgcc ttaccccgcg ttgcctgccg aagcccgccg cgcgctggat gagggtgtaa
    14761 tctgcgatat gtttgaaggt catgcgccgt acaaaccgcg ctatgtctta cccgattacg
    14821 cccgttttct ggcgaacggt tccgaatggc tggagctgga aggcgcgaaa gatcttgatg
    14881 acgcactctc tctgctgacc attctttacc accacgtacc gtcggtcaca tcgatgccgg
    14941 tctacctggg gcaactggat gcgttgttgc aaccgtatgt tagaattcta acacaagacg
    15001 agatcgatat tcgaataaaa cgtttctggc gttacctcga cagaaccctg ccagacgcct
    15061 ttatgcacgc caatatcggc ccgtctgatt cgcccatcac ccgtgcaatc ttacgtgcag
    15121 atgcagagct gaagcaggtt tcaccaaacc tgacctttat ctacgatcct gaaatcaccc
    15181 ctgatgacct gctgctggaa gtggcgaaga acatctgtga atgtagcaaa ccgcacatcg
    15241 ccaacggtcc ggtgcatgat aaaattttca caaaaggggg ctacgggatt gtgagctgtt
    15301 acaactcact gccgctggcg ggtggtggca gcacgctggt acgcctaaac ctgaaagcca
    15361 ttgccgagcg cagtgaatcg ctggaggact tctttacgcg cactctaccg cactactgcc
    15421 aacagcagat cgccatcatc gatgcgcggt gtgaattcct ctatcaacaa tcacacttct
    15481 ttgagaatag cttcctggtg aaagaagggc tgattaaccc tgaacgtttt gtgccaatgt
    15541 ttggcatgta cggactggcg gaagcagtta acttactgtg tgagaaagaa ggaattgtcg
    15601 cacgttacgg taaagaagcc accgcaaatg aagtgggtta tcgcatcagc gcgcaactgg
    15661 cggagtttgt cgccaatacc cctgtgaaat atggctggca aaaacgcgcc atgttacacg
    15721 cacagtcggg gatcagttcc gatatcggca ccacgccggg cgcgcgttta ccatatggcg
    15781 atgagccaga tccgattacc catctgcaaa ctgtcgcgcc gcatcatgct tattattatt
    15841 ccggcatcag cgacattctg acgctcgacg aaaccatcaa acgtaatccg caggcgctgg
    15901 tacagctttg cctcggtgcc tttaaagccg gaatgcgtga atttaccgcc aatgtcagcg
    15961 gtaacgatct ggttcgcgtt accggttata tggtgcgttt gtcggattta gaaaaatatc
    16021 gcgccgaagg ttcacgcacc aacaccacct ggctgggaga agaagccgca cgcaacactc
    16081 gtattctgga acgtcagccg cgcgtgataa gccatgaaca gcagatgcgc tttagtcagt
    16141 aagattatcc ccttctcctg cgttgacggg ccaggcagtc gtctggctct gtttttgcag
    16201 ggctgcaatc tgcgctgcaa aaactgtcac aatccgtgga cgatggggcg ctgtaatgat
    16261 tgcggagagt gcgtgccgca gtgtccgcat caggcgttgc agattatcga aggcaaagtg
    16321 gcgtggagcg cggctgtttg cgagcaatgt gatacctgcc tgaagatgtg cccgcaacat
    16381 gccacgccaa tggcgcaatc catgagcgtg gacgaggtgc ttaaccatgt ccgcaaagcg
    16441 gtgctgttta ttgaagggat aacggtaagc ggcggcgaag ccacgaccca gttgccgttt
    16501 gtggtggcgc tgtttactgc catcaaaaac gatccgcaac tgtgccatct cacctgcctc
    16561 gtggacagta acggcatgtt gagcgaaacc ggctgggaaa aattgctccc ggtgtgtgac
    16621 ggcgcaatgc tcgatctcaa agcgtggggg agcgaatgtc atcagcaatt aaccggacgc
    16681 gataatcagc agatcaagcg cagcatctgt ttgctggcag agcgcggtaa gctggcggaa
    16741 ctgcgtttgc tggtaatccc tggccaggtg gattatttgc aacacatcga agaactggcg
    16801 gcgttaatca agggacttgg cgatgttccg gtacgcctga acgcgtttca tgcccacggc
    16861 gtgtatggcg aggcgcaaag ctggccgagc gccacgccgg aggatgtgga acaactggct
    16921 gatgcgttac gggagcgcgg ggtgagtcgg ttaatatttc cggcgctata tttgtgacat
    16981 gccggatgtg gcctacgaag ggcaaactac ccacgcatgt taaacacctc attcgtatta
    17041 ttaagcaaca cttcggcaat ctcatccgcg ggttccggtc gtaactcgca tagcacagca
    17101 aacacacggg ctgcctgttc agggcgatta ggctgcccct gaaaaccatt gagcggcata
    17161 tctggtgcgt cggtttccag caacaacgat gccagcggca gttttgcgat gacatcgcgg
    17221 gttttactgg cgcgtggata ggtgatagta ccgcctacgc cgattttgta gcccagctgt
    17281 acaaaccgtt cagcctgttg ctggctgcca gaaaatccgt ggacaacacc agcacgcgat
    17341 aaatcgtggc gtttaagatg catcgccagt ttgtcgtgcg tgcgccgtga atgcaggatc
    17401 accggcagat cgtagcgttt cgccagtttc agttgttcat caagcaacca ctgctgcctc
    17461 tcaaattgcg gatcgtcacc aaagagatcc aggccgatct cccccaccgc caccaccttc
    17521 gccggatgcc tttccagcgc ctgctgtagc tgatcaagag acacatcgct atgtttttcc
    17581 aacataccag gatgcaagcc caatgcggca tacagcggtt gatatttttc cgctaacgcc
    17641 tgcacgcggg caaaattttc cgcctctgtc gccggaacaa tgatcttgcc tacgccagct
    17701 tgtgccgcac gttgcaggct ggcctcttca tcgccactaa acggcgggaa atcaaaatgg
    17761 cagtgggtgt cgataaaacg gcaaatcaag ccagatcctc attgttaaat gtcgggtcgt
    17821 tcgcctgcgg agcgtcgctc acttcggcaa ccaacgtatc gttggcgact ggcgcaggcg
    17881 gaataacgat cgactccggc gtcaccaccg gcaccagatg gcggctcagc ggcgcttttt
    17941 cagtcaataa tttaccgacc gttgccagaa aataacgccc acataaacgc ccaagtttat
    18001 agtcttcacg cagcgccgga atccgactgc cgagcgcaat actatgcaat ggcttcggcg
    18061 gataaatttc gaatatccgc agctggcccg gtggtttctc aataaattgc tgaatctcac
    18121 gatagctggt ttcatgatgc tgcaccagat tgaccagcgg ctgcaggcta ctgtcaccca
    18181 gccagcgttc catgcgtttg aaccactgcg gcgtgtagta catttgtgac ggcacagtgc
    18241 ggatgacgac caacgttttt gctccctgcc ttgccgcctc tttaaccggg atcgcatcac
    18301 tgatcccgcc atccaggtag ttaatgcctt ccagcgacac tccgctacga taaaagccag
    18361 gtatcgcact ggaggcgcga atcacatcca gccagttttg tttggttggt aaaaagtaat
    18421 tcggcgcgta gtcatcctga cgacaggcgc acatataaaa cgatttgccg ctgtcaaaca
    18481 accgcgcggc ggtatccatt tgcaacggca tctggcttgc ggtggcctcc accagccagt
    18541 cgagatcgat aagatttcct ccacggacaa agcgcaatgg atcgaaaaat tcgcgttttg
    18601 tggtatagcg catgatgact ttgcgcgcgt aaccgggctg attacagata tacgccgaga
    18661 ggttctgcgc cccggcagat gtgccgagat aaagatcgaa aggattaaac tgcgcgcgca
    18721 taaactcatc cagcacgcca gccgtgaaaa ttccacgctg tccgccgcct tcgcacacca
    18781 gagcaatccg tccaggctgg aacggcctta gcgacaacgg cgcaatatta ccaagcgtta
    18841 caggtattcg ctgccccacc tctgccttcc tgtttttgtt gtgaaagaat gacaaagtaa
    18901 cgcaatttat tattcgctaa aaccgcaaaa gccagtccgc tggactggct ttggataaaa
    18961 ttttcttaat ggtctgaaat ctagggtcgt tttcggccca tgaacaaact caccaggaac
    19021 agaataatcc cgacgacaaa gacaatttta gctgcgcctg cagcagtacc ggccagacca
    19081 ccaaacccaa gtgcggcggc gattaacgcg ataaccagaa atatgatgcc ccaacgaaac
    19141 ataagcgtct cctttaccat agttaatgtc accgcttgat atgagcgtga aaatcactca
    19201 atgcgatatt tttagtgtgg cgcacatcgc gcctcccgac aaagttcggg agatcgaatt
    19261 acgacgaatt acttagtttt cagatcattt ttaacgcttt tcacaccatc taccgctttg
    19321 gcgatacttt cagcacggtc gctctgtgcc tgagaatcta cggtaccgga gagctggacc
    19381 acgccatcgg tggtttcaac tttcacatga cgggaaggga cgatatcgtc cgccagcagt
    19441 ttggctttga tttcactggt ggtggcggtg tcacccgcgt agcctttcac agaactttct
    19501 ttagcgtcgc gaacgtgcag tttgtcgctg acagaggtca cgccttcaac gcctttcgcc
    19561 actttcactg cctcttcggc ctgggcctgg ctttcaacga aaccgctcag ggtcacgact
    19621 ttttgatcgg ttttgacaga gatatcggtg ctcttgatgt tgtcatgatc caccagggcc
    19681 gccttcactt tcgcggtgat ggcgctgtca tccatgaaat taccgacttt attcatagag
    19741 ctatcgactt tttgccctgc gctttcattg gtagtctgcg cgttgttttc cgcgtaggca
    19801 gagccggtcg cgacggcaga ggtcaacatt acagccagca aagttttcga aatcttcagt
    19861 cttgtcatag tcatcgattt attcctgtat gtttgctcgt aatttgagcc tggcaacacg
    19921 aggttgcatt gctgaatagg gagagacttc accctctaca aaagtcaatg gccgccatca
    19981 caaaagcgat gggtgatgaa taacgaccat tacagcctct gaatcagtta ttaatatcgg
    20041 tagaatgaca atcgacggct ttagatactg atatctacgc attgaacggt atttaacgcc
    20101 gtcagaaatg tcattacttt gttaaatata gatcacaatt ttgaaaccgc tcgggatatc
    20161 agcgaaaaca taagcaaaag tgaatgtttt aagaacattc cgtaagcggc taataaggaa
    20221 ggaaaattga cagggcgcag cggctaccgc gccctggagg caagaattaa tgctcgcggg
    20281 tctggtggaa ctgaacgtcc ggataacgtt cctgcgccag gcgcaggttg accatgctgg
    20341 tagcgatgta agcgaggtta tcgccgccat caagcgccag ttggctttcg ttcttacgct
    20401 tgaactcttc gaatttcttc gcgtccgcac attctaccca gcgggcagtg gcaacgttga
    20461 ctgactcata cactgcttca acgttgtatt cgctcttcag gcgcgctacc accacatcaa
    20521 actgcagcac accaactgca ccaacgatca ggtcgttgtt ggagatcgga cggaacacct
    20581 gcaccgcgcc ctcttcggaa agctgtacca gccctttgag cagctgtttt tgcttcagcg
    20641 gatctttcag gcggatacga cggaacagtt ctggcgcgaa gttcggaata ccggtgaact
    20701 tcatcatctc accctgggta aaggtgtcgc cgatctgaat ggtgccgtgg ttgtgcagac
    20761 caaggatatc gccaggatac gcttcttcaa cgtgcgaacg gtcacccgcc ataaaggtca
    20821 gcgcgtcgga gatcaccaca tctttcgcag tacgcacctg gcgcagcttc atgccttttt
    20881 catatttacc ggacaccaca cgcataaacg ccacgcggtc gcggtgtttc gggtccatgt
    20941 tggcctgaat tttaaatacg aagccggtaa atttatcttc gctcgcctct acggtacggg
    21001 tatcagtctg acgcggcatc ggcgctgggg cccactccac caggccatcc agcatatgat
    21061 caacgccgaa gttacccagc gcagtaccaa agaacactgg agtaatttcg cccgcaagga
    21121 acaattcttt gtcgaactcg ttagacgcgc ctttcaccag ttccagttcg tcacgcagtt
    21181 gctgtgccag atcttcacca accgcagcgt cgagatccgg gttattcagc cctttaacaa
    21241 tgcggacttc ctgaatggtg tggcctttac cgctctgata gagataggtt tcatctttat
    21301 aaaggtggta aacgccttta aacagcttgc cgcagccaat cggccaggtg atcggtgcgc
    21361 agccgatttt cagctcgttc tcaacttcat cgagcagttc catcgggtcg cggatatcac
    21421 ggtcaagttt gttcataaag gtgaggatcg gcgtatcgcg cagacgggta acttccatca
    21481 gcttacgggt acgatcttca acaccttttg cagcgtcgat aaccatcagg cagcagtcca
    21541 ccgccgtcag ggtacgatag gtatcttccg agaagtcttc gtgccccggg gtgtcgagca
    21601 ggttaaccag gcaatcgtga tacggaaact gcatcacaga agtagtaatg gagatcccac
    21661 gctgcttttc catctccatc cagtccgact tagcgtgctg gttggaacca cggcctttta
    21721 ctgtaccggc ggtctgaatg gcctgtccga acagcagcac cttctcggtg atggtagttt
    21781 taccggcgtc cgggtgagaa ataatggcaa aagtgcggcg cttcgccacc tcttgcaaat
    21841 aaggagacaa cgtcataatt ctttcttctt gagtaagcgg catcgcgccg cgcttgttga
    21901 aaacgaaaaa ttgcggctat tttacccatc aacggggggg aggcaatcag tgtttacaca
    21961 ggagctgctc cagttcgtgc aacgaagaaa cggtccaggt gggcgcgatg ccttctggtt
    22021 gctcgcgatt gtgcgcattc agccagcagg tcgcaagccc ggcgttgatg ccaccgagaa
    22081 tatcggactc ggcagtgtcg ccaaccatca gcacgcgtga acggtcagga ttgcccgcct
    22141 gttccagcgc ataatcgaaa attttcttat tcggtttggc aacgccaact tcttcggaaa
    22201 tcaccagcaa atcgaaataa tcacgcaggc ccgtgcgttc cagacgcacc tgttgcaagg
    22261 cgctaaaacc gttggtgatg atgccgattt tggcattgcc acgaatggcg ttaagcagag
    22321 aaaccgcgcc cggcaacggc gtacagattt ccgccatcgc attaataaag gcctcgttga
    22381 gtttacctgg ctcgacgttc agccgttcgg cccagctctc aaaacgcccg tgctgaagct
    22441 gtaatgaagt gatcgcgccg ttttgataat ctacccacag gggcttgtta acggcctgat
    22501 agtcctgaaa atcttcagcg gtaaaagtga cgctgtaatc aagaaacatc cgctgcagtc
    22561 cggtgaacga gtcaaaggta aacagcgttt catcggcatc aaagaaaatc cagtcccact
    22621 tcattattcc accttgtctt acatactgat tggcaacgcc atgatgatgg cgtcttcgcg
    22681 accgtccgtg gtggggtagt aattgcggcg aattgtcgcc tcgttaaagc ctaaactttc
    22741 gtacagggca atggcggcag cgtttgaagc acggacttcc agccatagcg tcgccacgcc
    22801 gcgtttttcc agttcgtcga tcagatgttc cagcagcgcc cttcccaatc cctgacgctg
    22861 atagtcagga tcgaccgcaa tattgaacaa tgtagcttca tccagcacca cttgcgtaat
    22921 cgcaaacgcc gccattttgc cgttttgcgt taactgaaag ttgagataac gctcgccctg
    22981 attactggca aaggtttttt cactccacgg aaaggcgtgg gcgcgttgtt caatgtggta
    23041 agccgctggt aaatcagtcg tttcgaggga agaaatcgtg ttcatatgcg caaatttgtt
    23101 gccataacgc ggcgcgtgcc gttgggtttg cccgtaattc ggtgagcgcc ggtgatgcca
    23161 cctgagcgcc ttccagtgat agcggttcgt cagtacccaa ccgccagctg ttgcagcgac
    23221 tgccttgcgg cagcatcgcg attttttctg gcgtcagttg cagcacttga tcggggctga
    23281 cggttaatgc gcgcagaaca tcgctcacta aaggatcagt cagggcggga agatcgtttg
    23341 ccaccatcac cagacggacg tgtgccggga tcgcaatggc aatctccccc tgcaacgcgc
    23401 cagggcgacg cagcgaccac tgggtaatgc ccagttgctg taactgccag tctcgtcggg
    23461 atgtcatagc gaatcgctcc tgttgtcagg ggcgcaaata tagcaaattc gtcgataccg
    23521 cgccaacata tggctataat cgccgccagt atcaattgag gagcattcca tgtctgcatt
    23581 taccccggca agtgaagtct tgctgcgtca cagtgatgat ttcgaacaaa gccgtattct
    23641 gtttgccgga gacttacagg atgacctgcc cgcgcgttta gataccgcgg ccagccgtgc
    23701 tcatacccag caattccacc actggcaggt gttaagccgc cagatggggg ataacgcccg
    23761 ttttagtctg gtcgccacgg cgaatgatgt cgcagattgc gatacgctga tttactactg
    23821 gccgaagaac aaaccggaag cccagttcca gttgatgaat ttactttctc tgctgccagt
    23881 gggcacggat atttttgtcg ttggcgagaa
//
LOCUS       DAMZFT010000076        23290 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.076, whole genome shotgun sequence.
ACCESSION   DAMZFT010000076 DAMZFT010000000
VERSION     DAMZFT010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 23290)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23290)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23290
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.076"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            39..881
                     /gene="chbR"
                     /locus_tag="PN977_003056"
     CDS             39..881
                     /gene="chbR"
                     /locus_tag="PN977_003056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ChbR"
                     /protein_id="HDI6043068.1"
                     /translation="MMQPMINAPEIATAREQQLFNGKNFHVFIYNKTESISGLHQHDY
                     YEFTLVLTGRYFQEINGKRVLLERGDFVFIPLGSHHQSFYEFGATRILNVGISKRFFE
                     QHYLPLLPYCFVASQVYRTNNAFLTYVETVISSLNFRETGLEEFVEMVTFYVINRLRH
                     YREEQVIDDVPQWLKSTVEKMHDKEQFSESALENMVTLSAKSQEYLTRATQRYYGKTP
                     MQIINEIRINFAKKQLEMTNYSVTDIAFEAGYSSPSLFIKTFKKLTSFTPKSYRKKLT
                     EFNQ"
     gene            985..2337
                     /gene="celF"
                     /locus_tag="PN977_003057"
                     /gene_synonym="chbF"
     CDS             985..2337
                     /gene="celF"
                     /locus_tag="PN977_003057"
                     /gene_synonym="chbF"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HDI6043069.1"
                     /translation="MSQKLKVVTIGGGSSYTPELLEGFIKRYHELPVSELWLVDVEDG
                     KEKLDIIFDLCQRMIDNAGVPMKLYKTLDRREALKDADFVTTQLRVGQLPARELDERI
                     PLSHGYLGQETNGAGGLFKGLRTIPVIFDIVKDVEELCPNAWVINFTNPAGMVTEAVY
                     RHTGFKRFIGVCNIPIGMKMFIRDVLMLKDSDDLSIDLFGLNHMVFIKDVLVNGKSRF
                     AELLDGVASGQLKASGVKNIFDLPFSEGLIRSLNLLPCSYLLYYFKQKEMLAIEMGEY
                     YKGGARAQVVQKVEKQLFELYKNPELKVKPKELEQRGGAYYSDAACEVINAIYNDKQA
                     EHYVNIPHHGHIDNIPADWAVEMTCTLGRDGATPHPRITHFDDKVMGLIHTIKSFEIA
                     ASNAALSGEFNDVLLALNLSPLVHSDRDAELLAREMILAHEKWLPNFADCIAELKKAH
                     "
     gene            2350..3099
                     /gene="chbG"
                     /locus_tag="PN977_003058"
     CDS             2350..3099
                     /gene="chbG"
                     /locus_tag="PN977_003058"
                     /EC_number="3.5.1.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitin disaccharide deacetylase"
                     /protein_id="HDI6043070.1"
                     /translation="MERLLIVNADDFGLSKGQNYGIIEACRNGIVTSTTALVNGQAID
                     HAVQLSRDEPSLAIGMHFVLTMGKPLTAMPGLTRDGVLGKWIWQLAEEDALPLEEITQ
                     ELASQYLRFIELFGRKPTHLDSHHHVHMFPQIFPIVARFAAEEGIALRADRQMVFDLP
                     VNLRTTQGFSSAFYGEEISESLFLQVLDDSSHRGERSLEVMCHPAFIDNTIRQSAYCF
                     PRLTELDVLTSASLKYAIAERGYRLGSYLDV"
     gene            complement(3146..5407)
                     /gene="katE"
                     /locus_tag="PN977_003059"
     CDS             complement(3146..5407)
                     /gene="katE"
                     /locus_tag="PN977_003059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase HPII"
                     /protein_id="HDI6043071.1"
                     /translation="MSQHNEKNPHQHQSPLHDSSEAKPGMDSLAPEDGSHRPAAEPTP
                     PGAQPTAPGSLKAPDTRNEKLNSLEDVRKGSENYALTTNQGVRIADDQNSLRGGSRGP
                     TLLEDFILREKITHFDHERIPERIVHARGSAAHGYFQPYKSLSDITKADFLSDPNKIT
                     PVFVRFSTVQGGAGSADTVRDIRGFATKFYTEEGIFDLVGNNTPIFFIQDAHKFPDFV
                     HAVKPEPHWAIPQGQSAHDTFWDYVSLQPETLHNVMWAMSDRGIPRSYRTMEGFGIHT
                     FRLINAEGKATFVRFHWKPLAGKASLVWDEAQKLTGRDPDFHRRELWEAIEAGDFPEY
                     ELGFQLIPEEDEFKFDFDLLDPTKLIPEELVPVQRVGKMVLNRNPDNFFAENEQAAFH
                     PGHIVPGLDFTNDPLLQGRLFSYTDTQISRLGGPNFHEIPINRPTCPYHNFQRDGMHR
                     MGIDTNPANYEPNSINDNWPRETPPGPKRGGFESYQERVEGNKVRERSPSFGEYYSHP
                     RLFWLSQTPFEQRHIVDGFSFELSKVVRPYIRERVVDQLAHIDLTLAQAVAKNLGIEL
                     TDDQLNITPPPDVNGLKKDPSLSLYAIPDGDVKGRVVAILLNDEVRSADLLAILKALK
                     AKGVHAKLLYSRMGEVTADDGTVLPIAATFAGAPSLTVDAVIVPCGNIADIADNGDAN
                     YYLMEAYKHLKPIALAGDARKFKVRIKVADQGEEGIVEADSADGSFMDELLTLMAAHR
                     VWSRIPKIDKIPA"
     gene            5590..5853
                     /gene="cedA"
                     /locus_tag="PN977_003060"
     CDS             5590..5853
                     /gene="cedA"
                     /locus_tag="PN977_003060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division activator CedA"
                     /protein_id="HDI6043072.1"
                     /translation="MRLVKPVMKKPLRQQNRQIISYIPRTEPAPPEHAIKMDSFRDVW
                     MLRGKYVAFVLMGESFMRSPAFTVPESAQRWANQIRQEGEVTE"
     gene            6142..6945
                     /locus_tag="PN977_003061"
     CDS             6142..6945
                     /locus_tag="PN977_003061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6043073.1"
                     /translation="MLSTNLIQSNYGDLNIKSLAFDSFKERLQSTMTALTFFISTGQC
                     DCDEIAESNFNYMIAYMSNINYDASKPGAPALSFDTYLQDNVKYRVIINNLYGSEIRI
                     RGINKDFIGMDVTSVFRPEKMTNLINIKNRLVIHYLRTIYYEQNYIHPVGSIFAAIQK
                     NESLLKFPSIISMLNISLLFNPLNLPGMGNGVLEDIMSISDSSLRKRLGYEVLSFSLQ
                     AHSLSQECIDKLAIFFADDLFQYESVCIAAMEHLKSKATVPIQNGPLPA"
     gene            complement(6949..8340)
                     /gene="tcyP"
                     /locus_tag="PN977_003062"
     CDS             complement(6949..8340)
                     /gene="tcyP"
                     /locus_tag="PN977_003062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine/sulfocysteine:cation symporter"
                     /protein_id="HDI6043074.1"
                     /translation="MNFPLIANIVVFVVLLFALAQTRHKQWSLAKKVLVGLVMGVVFG
                     LALHTIYGSDSQVLKDSVQWFNIVGNGYVQLLQMIVMPLVFASILSAVARLHNASQLG
                     KISFLTIGTLLFTTLIAALVGVLVTNLFGLTAEGLVQGGAETARLNAIESNYVGKVSD
                     LSVPQLVLSFIPKNPFADLTGANPTSIISVVIFAAFLGVAALKLLKDDAPKGERVLTA
                     IDTLQSWVMKLVRLVMQLTPYGVLALMTKVVAGSNLQDIIKLGSFVVASYLGLLIMFA
                     VHGLLLGINGVSPLKYFRKVWPVLTFAFTSRSSAASIPLNVEAQTRRLGVPESIASFA
                     ASFGATIGQNGCAGLYPAMLAVMVAPTVGINPMDPMWIATLVGIVTVSSAGVAGVGGG
                     ATFAALIVLPAMGLPVTLVALLISVEPLIDMGRTALNVSGSMTAGTLTSQWLKQTDKA
                     ILDSEDDAELAHR"
     gene            complement(8473..9063)
                     /locus_tag="PN977_003063"
     CDS             complement(8473..9063)
                     /locus_tag="PN977_003063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310461.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HDI6043075.1"
                     /translation="MTAEGHLLFSIACAVFAKNAELTPVLAQGDWWHIVPSAILTCLL
                     PDIDHPKSFLGQRLKWISKPIARAFGHRGFTHSLLAVFALLATFYLKVPESWFIPADA
                     LQGMVLGYLSHILADMLTPAGVPLLWPCRWRFRLPILVPQKGNQLERFICMALFVWSV
                     WMPHSLPENSAVRWSSQMINTLQIQFHRLIKHQVEY"
     gene            complement(9226..9894)
                     /gene="hxpB"
                     /locus_tag="PN977_003064"
     CDS             complement(9226..9894)
                     /gene="hxpB"
                     /locus_tag="PN977_003064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpB"
                     /protein_id="HDI6043076.1"
                     /translation="MSTPRQILAAIFDMDGLLIDSEPLWDRAELDVMASLGVDISRRN
                     ELPDTLGLRIDMVVDLWYARQPWNGPSRQEVVERVIARAISLVEETRPLLPGVREAVA
                     LCKEQGLLVGLASASPLHMLEKVLTMFDLRDSFDALASAEKLPYSKPHPQVYLDCAAK
                     LGVDPLTCVALEDSVNGMIASKAARMRSIVVPAPEAQNDPRFVLANVKLSSLTELTAK
                     DLLG"
     gene            10041..10577
                     /locus_tag="PN977_003065"
     CDS             10041..10577
                     /locus_tag="PN977_003065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YniB family protein"
                     /protein_id="HDI6043077.1"
                     /translation="MTYQQAGRIAVLKRILGWVIFIPALISTLISLLKFMNTRQENQE
                     GINAVMLDFTHVMIDMMQANTPFLNLFWYNSPTPNFNGGVNVMFWMIFILIFVGLALQ
                     DSGARMSRQARFLREGVEDQLILEKAKGEEGLTREQIESRIVVPHHTIFLQFFSLYIL
                     PVICIAAGYVFFSLLGFI"
     gene            complement(10618..11478)
                     /locus_tag="PN977_003066"
     CDS             complement(10618..11478)
                     /locus_tag="PN977_003066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HDI6043078.1"
                     /translation="MWQAISRLLSEQLGEGEIELRNELPGGEVHAAWHLRYAGRDFFV
                     KCDERELLPGFTAEADQLELLSRSKTVTVPKVWAVGADRDYSFLVMDYLPPRPLDAHS
                     AFILGQQIARLHQWSDQPQFGLDFDNALSTTPQPNTWQRRWSTFFAEQRIGWQLELAA
                     EKGIAFGNIDAIVEHIQQRLASHQPQPSLLHGDLWSGNCALGPDGPYIFDPACYWGDR
                     ECDLAMLPLHTEQPPQIYDGYQSVSPLPADFLERQPVYQLYTLLNRARLFGGQHLVIA
                     QQSLDRLLAA"
     gene            complement(11584..11874)
                     /gene="ghoS"
                     /locus_tag="PN977_003067"
     CDS             complement(11584..11874)
                     /gene="ghoS"
                     /locus_tag="PN977_003067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system endoribonuclease
                     antitoxin GhoS"
                     /protein_id="HDI6043079.1"
                     /translation="MASGDLVRYVITVMLHEDTLTEINELNNYLTRDGFLLTMTDDDG
                     NIHELGTNTFGLISTQSEEEIRELVSGLTQSATGKDPEITITTWEEWNSNRK"
     gene            complement(11975..12904)
                     /gene="pfkB"
                     /locus_tag="PN977_003068"
     CDS             complement(11975..12904)
                     /gene="pfkB"
                     /locus_tag="PN977_003068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416237.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase II"
                     /protein_id="HDI6043080.1"
                     /translation="MVRIYTLTLAPSLDSATITPQIYPEGKLRCTAPVFEPGGGGINV
                     ARAIAHLGGSATAIFPAGGATGEHLVSLLADENVPVATVDAKDWTRQNLHVHVEASGE
                     QYRFVMPGAALNEDEFRQLEEQVLEIESGAILVISGSLPPGVKLEKLTQLISAAQNQG
                     IRCIVDSSGEALSAALAIGNIELVKPNQKELSALVNRELTQPDDVRKAAQEIVNSGKA
                     KRVVVSLGPQGALGVDSENCIQVVPPPVKSQSTVGAGDSMVGAMTLKLAENASLEEMV
                     RFGVAAGSAATLNQGTRLCSHDDTQKIYAYLSR"
     gene            13191..13949
                     /locus_tag="PN977_003069"
     CDS             13191..13949
                     /locus_tag="PN977_003069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiY family protein"
                     /protein_id="HDI6043081.1"
                     /translation="MKLLKTVPAIVMLAGGMFASLNAAADDSVFTVMDDPASAKKPFE
                     GNLNAGYLAQSGNTKSSSLTADTTMTWYGQTTAWSLWGNASNTSSNDERSSEKYAAGG
                     RSRFNLTDYDYLFGQASWLTDRYNGYRERDVLTAGYGRQFLNGPVHSFRFEFGPGVRY
                     DKYTDNASETQPLGYASGAYAWQLTDNAKFTQGVSVFGAEDTTLNSESALNVAINEHF
                     GLKVAYNVTWNSEPPESAPEHTDRRTTLSLGYSM"
     gene            complement(14002..14109)
                     /locus_tag="PN977_003070"
     CDS             complement(14002..14109)
                     /locus_tag="PN977_003070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588455.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6043082.1"
                     /translation="MPTKRFDKKHWKMVVVLLAICGAMLLLRWAAMIWG"
     gene            complement(14284..16182)
                     /gene="espL1"
                     /locus_tag="PN977_003071"
     CDS             complement(14284..16182)
                     /gene="espL1"
                     /locus_tag="PN977_003071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector EspL1"
                     /protein_id="HDI6043083.1"
                     /translation="MSQNDIIIRTHYKSPHRMHIDSDIPTPSSEPINQFARQLITLLD
                     TSDLSSMLTYCVTQEFTANCRKISQNCYSTALFTINFATSPIHAENILITLHYKKEII
                     SLLLETTPIKANHLRSILDYIEQEQLTAENRNHCMKLSKKIHREKTIQPTVNLNGSAF
                     FLQSPSDAIFCRHLSLQYALDSLRNGKGKVNLIKHYSSVESIQQHVPLVRDAEFRSLL
                     RHPPAGSRVIASKDFGFALDIFFCRMMANNVSHMSAILYIDNHTLSVRLRIKQSAYGQ
                     LNYVVSVYDPNDTNVAVRGTHRTARGFLSLDKFISSGPDAQTWADMYVRNCAIAFLPL
                     LPEGVPGAIFAGIASRMPFAPIHPSAMLLIMATGQTQQLITLFKQLPILPEKEINEII
                     TAQNSVGTPALFLAMMNGHTDNVKIFMQEIQSLVDNHIIHEDNLVKLLQTKSANETPG
                     LYISMLYGFDEIIDIFLNALTTPIAQDLLNKKMVMNILAMKTRDGEPGLYAAMENNHP
                     LCVTRFLSKVYGIAVKYNLSKINIMDLLKGATAHGTPALYIAMSKGNKDVVLSYISTL
                     GTFAKKYSFSQCQLFTLLAAKNHDNMSAVHIAIHHNHYKTVETYYAAINVISQSLSFS
                     PDELQAYL"
     gene            16705..18633
                     /gene="thrS"
                     /locus_tag="PN977_003072"
     CDS             16705..18633
                     /gene="thrS"
                     /locus_tag="PN977_003072"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006894721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HDI6043084.1"
                     /translation="MPVITLPDGSQRHYDHAVSPMDVALDIGPGLAKACIAGRVNGEL
                     VDACDLIENDAQLSIITAKDEEGLEIIRHSCAHLLGHAIKQLWPHTKMAIGPVIDNGF
                     YYDVDLDRTLTQEDVEALEKRMHELAEKNYDVIKKKVSWHEARETFANRGESYKVSIL
                     DENIAHDDKPGLYFHEEYVDMCRGPHVPNMRFCHHFKLMKTAGAYWRGDSNNKMLQRI
                     YGTAWADKKALNAYLQRLEEAAKRDHRKIGKQLDLYHMQEEAPGMVFWHNDGWTIFRE
                     LEVFVRSKLKEYQYQEVKGPFMMDRVLWEKTGHWDNYKDAMFTTSSENREYCIKPMNC
                     PGHVQIFNQGLKSYRDLPLRMAEFGSCHRNEPSGSLHGLMRVRGFTQDDAHIFCTEEQ
                     IRDEVNGCIRLVYDMYSTFGFEKIVVKLSTRPEKRIGSDEMWDRAEADLAVALEENNI
                     PFEYQLGEGAFYGPKIEFTLYDCLDRAWQCGTVQLDFSLPSRLSASYVGEDNERKVPV
                     MIHRAILGSMERFIGILTEEFAGFFPTWLAPVQVVIMNITDSQSEYVNELTQKLSNAG
                     IRVKADLRNEKIGFKIREHTLRRVPYMLVCGDKEVESGKVAVRTRRGKDLGSMDVNEV
                     IEKLQQEIRSRSLKQLEE"
     gene            18637..19179
                     /gene="infC"
                     /locus_tag="PN977_003073"
     CDS             18637..19179
                     /gene="infC"
                     /locus_tag="PN977_003073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HDI6043085.1"
                     /translation="MKGGKRVQTARPNRINGEIRAQEVRLTGLEGEQLGIVSLREALE
                     KAEEAGVDLVEISPNAEPPVCRIMDYGKFLYEKSKSSKEQKKKQKVIQVKEIKFRPGT
                     DEGDYQVKLRSLIRFLEEGDKAKITLRFRGREMAHQQIGMEVLNRVKDDLQELAVVES
                     FPTKIEGRQMIMVLAPKKKQ"
     gene            19276..19473
                     /gene="rpmI"
                     /locus_tag="PN977_003074"
     CDS             19276..19473
                     /gene="rpmI"
                     /locus_tag="PN977_003074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006708231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HDI6043086.1"
                     /translation="MPKIKTVRGAAKRFKKTGKGGFKHKHANLRHILTKKATKRKRHL
                     RPKAMVSKGDLGLVIACLPYA"
     gene            19527..19883
                     /gene="rplT"
                     /locus_tag="PN977_003075"
     CDS             19527..19883
                     /gene="rplT"
                     /locus_tag="PN977_003075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HDI6043087.1"
                     /translation="MARVKRGVIARARHKKILKQAKGYYGARSRVYRVAFQAVIKAGQ
                     YAYRDRRQRKRQFRQLWIARINAAARQNGISYSKFINGLKKASVEIDRKILADIAVFD
                     KVAFTALVEKAKAALA"
     misc_feature    20000..20124
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            20006..20050
                     /gene="pheM"
                     /locus_tag="PN977_003076"
     CDS             20006..20050
                     /gene="pheM"
                     /locus_tag="PN977_003076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pheST operon leader peptide PheM"
                     /protein_id="HDI6043088.1"
                     /translation="MNAAIFRFFFYFST"
     gene            20189..21172
                     /gene="pheS"
                     /locus_tag="PN977_003077"
     CDS             20189..21172
                     /gene="pheS"
                     /locus_tag="PN977_003077"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004851795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HDI6043089.1"
                     /translation="MSHLAELVASAKAAISQASDVAALDNVRVEYLGKKGHLTLQMTT
                     LRELPPEERPAAGAVINEAKEQVQQALNARKAELESAALNARLAAETIDVSLPGRRIE
                     NGGLHPVTRTIDRIESFFGELGFTVATGPEIEDDYHNFDALNIPGHHPARADHDTFWF
                     DATRLLRTQTSGVQIRTMKAQQPPIRIIAPGRVYRNDYDQTHTPMFHQMEGLIVDTNI
                     SFTNLKGTLHDFLRNFFEEDLQIRFRPSYFPFTEPSAEVDVMGKNGKWLEVLGCGMVH
                     PNVLRNVGIDPEVYSGFAFGMGMERLTMLRYGVTDLRSFFENDLRFLKQFK"
     gene            21187..>23290
                     /gene="pheT"
                     /locus_tag="PN977_003078"
     CDS             21187..>23290
                     /gene="pheT"
                     /locus_tag="PN977_003078"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HDI6043090.1"
                     /translation="MKFSELWLREWVNPAIDSDALANQITMAGLEVDGVEPVAGSFHG
                     VVVGEVVECAQHPNADKLRVTKVNVGGDRLLDIVCGAPNCRQGLRVAVATIGAVLPGD
                     FKIKAAKLRGEPSEGMLCSFSELGISDDHSGIIELPADAPIGTDIREYLKLDDNTIEI
                     SVTPNRADCLGIIGVARDVAVLNQLPLVEPEIVPVGATIDDTLPIAVEAPEACPRYLG
                     RVVKGINVKAPTPLWMKEKLRRCGIRSIDAVVDVTNYVLLELGQPMHAFDKDRIEGGI
                     VVRMAKEGETLVLLDGTEAKLNADTLVIADHNKALAMGGIFGGEHSGVNDETQNVLLE
                     CAFFSPLSITGRARRHGLHTDASHRYERGVDPALQHKAMERATRLLIDICGGEAGPVI
                     DITNEATLPKRATITLRRSKLDRLIGHHIADEQVTDILRRLGCEVTEGKDEWQAVAPS
                     WRFDMEIEEDLVEEVARVYGYNNIPDEPVQASLIMGTHREADLSLKRVKTLLNDKGYQ
                     EVITYSFVDPKVQQMIHPGVEALLLPSPISVEMSAMRLSLWTGLLATVVYNQNRQQNR
                     VRIFESGLRFVPDTQAPLGIRQDLMLAGVICGNRYEEHWNLAKETVDFYDLKGDLESV
                     LDLTGKLNEVEFRAEANPALHPGQSAAIYLKGERIGFVGVVHPELERKLDLNGRTLVF
                     ELEWNKLADRVVPQAREIS"
BASE COUNT         6082 a         5314 c         5820 g         6074 t
ORIGIN      
        1 aattgagctt catgaaaaac tgaaggcata aggagtcgat gatgcagcca atgattaacg
       61 cgccggaaat tgccactgcc cgagaacagc agttgtttaa tggcaaaaac ttccatgtgt
      121 ttatctacaa caaaactgag agtatcagcg gactgcatca gcacgactat tatgaattta
      181 ctctggtatt aaccgggcgt tatttccagg agattaacgg taagcgcgtg ttactggaac
      241 ggggcgattt tgtttttatt ccgttaggtt cgcaccatca aagtttttat gagtttggtg
      301 ccacgcgcat attgaacgtt gggatcagta aacgcttttt tgagcagcat tacctgccgt
      361 tgttgcctta ttgctttgtt gcttcgcagg tataccggac caataacgct tttctcacct
      421 atgtggaaac agtgatttct tcattgaatt tccgcgaaac agggctggaa gagtttgttg
      481 agatggttac tttttatgtc attaaccgtt tacgtcatta ccgcgaagaa caggtgattg
      541 atgatgtacc gcagtggctg aaaagtacgg tagaaaagat gcatgataaa gagcagttta
      601 gtgaatcggc actggagaat atggtgacgt tgtcagccaa atcacaggaa tatttgacgc
      661 gagcgactca acgatattat ggcaaaacgc caatgcagat tattaatgaa atccgtatta
      721 attttgccaa aaaacaactg gaaatgacca actattcagt gacggatatt gcgtttgagg
      781 ccggttatag tagcccgagt ttgtttatta aaacgtttaa gaaattaacg tcctttacgc
      841 ctaagagcta tcgtaagaaa ttgactgaat ttaatcagta agtcgttata cctgacaatt
      901 cacatatctg tcctgttgct ggattattta tgtccggggg gcagatatgc cagatatcag
      961 tattctgtac tgaagggaga aattatgagc cagaaattaa aagtcgtcac tattggtggc
     1021 gggagcagct ataccccgga gttactggaa ggatttatta agcgttatca cgaattgccg
     1081 gtcagcgaat tatggctggt ggatgtcgaa gatggtaaag agaaactgga tattattttt
     1141 gatctctgcc aacggatgat tgataacgct ggcgtcccga tgaagcttta taaaacgctg
     1201 gatcgccgcg aagcattgaa agatgctgat ttcgttacta cccaactgcg cgtaggccaa
     1261 ttaccggcgc gcgaactgga tgaacgtatt ccattaagtc atggttatct tggtcaggaa
     1321 accaacggcg cgggcggcct gtttaaaggt ctgcgtacca ttccggtgat ttttgacatc
     1381 gtaaaagatg tcgaagaact ttgtccgaat gcatgggtga ttaatttcac taacccggcg
     1441 ggaatggtca ctgaagccgt ttatcgtcat actggattta aacgctttat cggcgtgtgt
     1501 aatattccga tcggcatgaa gatgtttatt cgcgatgttc tgatgctgaa agacagcgat
     1561 gatttatcta tcgatctgtt cggcctcaac catatggtgt tcattaaaga tgtgctggta
     1621 aatggcaaat cacgctttgc cgaattgctt gatggtgtgg catccgggca gttaaaagca
     1681 tctggcgtta aaaatatttt cgatctgcca tttagcgaag gcttaattcg ttctctgaat
     1741 ctgttgccgt gttcttattt gctttattac ttcaagcaaa aagagatgct ggctattgaa
     1801 atgggcgaat actacaaagg cggcgcacga gcgcaggtag tacagaaagt cgagaaacaa
     1861 ctttttgagc tgtataaaaa cccggagttg aaagttaagc cgaaagaact ggaacagcgc
     1921 ggtggggctt attactctga tgctgcatgc gaagtgatca acgctatcta caacgacaag
     1981 caagctgaac attacgttaa tatcccgcat catgggcata ttgataatat tccggcagac
     2041 tgggcggtag aaatgacctg tacgctgggg cgcgatggcg cgacgccaca tccgcgcatt
     2101 acgcatttcg atgataaagt gatggggctg attcacacca ttaaaagctt cgagattgct
     2161 gccagcaacg ccgcacttag cggagagttt aacgatgtgt tactggccct aaaccttagt
     2221 ccgttggtgc attccgatcg cgatgctgag ctgctggcac gcgagatgat tctggcgcac
     2281 gagaaatggt tgccaaattt tgccgactgc atcgcagagc ttaaaaaagc acattaaccg
     2341 aggctgatta tggaacgctt actgattgtt aatgccgatg attttggttt aagcaaaggt
     2401 cagaactacg gcattatcga ggcctgtcgc aatgggattg tcacatcgac gacggcgctg
     2461 gtgaatgggc aggctattga tcatgcggtg cagttgagtc gtgatgaacc gagtctggcc
     2521 atagggatgc actttgtcct tactatgggc aagccactga cagctatgcc ggggttaacc
     2581 cgcgatggtg tgctgggaaa atggatctgg cagttggcag aagaagatgc tttaccgctg
     2641 gaagaaatta ctcaggagct cgccagtcag tatttgcgtt tcattgagct atttggacgc
     2701 aaacctacgc atcttgatag ccatcatcat gtgcatatgt tcccgcagat tttcccgatt
     2761 gttgccaggt ttgcggctga agagggcatt gcgttgcggg cggatcgtca gatggtgttt
     2821 gatttgccgg tcaacctgcg cactacccag ggattcagca gtgcattcta cggtgaagag
     2881 atcagtgagt cgctgttcct gcaagtgctt gatgactcca gtcatcgcgg cgagcgctct
     2941 ctggaagtga tgtgccaccc ggcgtttatc gataatacga ttcgccagag tgcttattgc
     3001 ttccctcggt taacggaact ggatgtgctg acttcagcgt cgttgaaata tgcgattgct
     3061 gagcgtggtt atcggttggg tagttatctt gatgtgtaag taaggtggta aggtcactcc
     3121 ccagggggcg tgaccttatc catatttacg ccggaatttt gtcaatctta ggaatgcgtg
     3181 accacacgcg gtgtgctgcc atcagcgtta acagttcatc cataaaacta ccatcggcgc
     3241 tgtcagcttc cacaatccct tcttcaccct gatcagcgac cttgattctt actttaaact
     3301 tgcgcgcgtc tcctgccagc gcaatcggtt taaggtgttt gtaggcttcc atcaggtagt
     3361 agttggcatc gccgttgtca gcgatatccg cgatattgcc gcaagggaca ataaccgcat
     3421 cgaccgtcag cgaaggcgct ccggcaaatg tagcggctat aggcagcacc gtaccgtcat
     3481 ccgcagtcac ttcacccatt cgggagtaga gcagtttggc atgaacgcct ttggccttca
     3541 gcgccttgag aatggccaga aggtctgccg atctcacttc atcattaagt aaaatcgcta
     3601 ccacgcgacc tttcacatca ccgtcaggaa tggcgtacaa acttaaggat ggatcctttt
     3661 tcagaccgtt gacgtccgga ggcggggtga tattcagctg gtcgtccgtc agctcgatac
     3721 cgagattttt cgccaccgcc tgggccagag tgagatcaat atgtgccagc tggtcaacaa
     3781 cgcgctcacg aatatacgga cgaactactt tgcttaactc aaaactgaaa ccatcgacaa
     3841 tatggcgctg ctcgaatggc gtctgactta gccagaacag acgcggatgg gaataatatt
     3901 cgccaaacga tgggctgcgc tcgcgaactt tattgccttc cacgcgctcc tggtatgatt
     3961 caaaaccgcc gcgtttcggc cccggcggtg tttcgcgcgg ccagttatcg ttgatcgagt
     4021 tcggttcata attcgccggg ttagtgtcga tccccatacg atgcatgccg tcacgctgga
     4081 aattatggta agggcaggtc gggcggttaa tcggaatctc atggaaattt ggtccgccaa
     4141 gacgactgat ttgtgtatcg gtataagaga acaaacgccc ctgcaacagc ggatcgttgg
     4201 tgaagtccag accaggaaca atatgacctg gatggaatgc tgcttgttcg ttttcggcaa
     4261 agaagttatc cggattgcga ttgagcacca ttttgccgac acgctgaacg ggcaccagtt
     4321 cttccgggat aagtttagtt ggatcaagaa ggtcgaagtc gaacttaaat tcatcttcct
     4381 caggaatcaa ctggaagccc agttcgtatt ccggaaaatc gccggcttca atcgcttccc
     4441 acaactcgcg gcggtggaag tccgggtcac gtccggttag tttttgtgct tcatcccaaa
     4501 cgagtgaggc tttacctgcc agtggtttcc agtggaaacg tacgaacgtc gctttacctt
     4561 cggcgttaat cagacggaag gtatgaatac caaagccttc catagtgcgg tagctgcgcg
     4621 ggataccacg atccgacatc gcccacatta cgttgtgcag tgtttccggt tgcagggaaa
     4681 cataatccca gaaggtgtcg tgagcgcttt gcccctgcgg gattgcccag tgtggttctg
     4741 gttttaccgc atgcacaaaa tcagggaatt tatgcgcatc ctggataaag aagattggcg
     4801 tgttattgcc aacaaggtca aaaataccct cttcggtata gaacttggtg gcaaagccac
     4861 ggatatcacg cacggtatcg gcagagccag caccaccctg aacggtagag aaacgtacaa
     4921 atactggggt gattttgttc ggatctgaga ggaaatccgc tttggtaata tcgcttaagc
     4981 ttttatatgg ctggaaataa ccgtgagcgg ctgatccacg tgcatgaaca atacgttccg
     5041 ggatgcgctc atggtcaaag tgggtgattt tctcgcgcag aataaaatct tccagcagcg
     5101 ttggaccacg gctaccgcca cgcagtgagt tttgatcgtc ggcgatgcgc acgccctgat
     5161 tagtggtcag cgcataattt tcactgcctt tgcgtacgtc ttccagagaa ttaagttttt
     5221 cgttacgcgt atcaggggct ttcaggctcc ctggggcggt aggttgtgca ccaggcggtg
     5281 ttggttcagc cgctggacga tgagatccgt cttcaggtgc cagtgagtcc atccccggtt
     5341 tcgcttcgct ggaatcgtgt agtggtgact ggtgctgatg tgggttcttt tcgttatgtt
     5401 gcgacattga actcgtctcc ttaaattatt actgaaaggg ccgctgtttt ggtcaaaaaa
     5461 ccctctaact atagaccacc agccagatcc cgcttcggaa actattctgg aaacggtgtt
     5521 atgaaataca cgcaaagcgg gacgtacagg ggctaaatcg gctaaactac agtttttctg
     5581 atttttaata tgcgtttagt gaagccagta atgaagaaac cgctccgtca gcaaaaccgc
     5641 cagattatta gctatatccc acgcacggaa cccgcgccgc cagaacatgc gataaagatg
     5701 gattcgtttc gtgatgtctg gatgctgcgt ggcaaatatg ttgcgtttgt actgatgggg
     5761 gagtcattta tgcgctcacc ggcgtttact gtgcctgaat ccgcccagcg ttgggcaaat
     5821 cagatccgcc aggaagggga agtgactgag taaagattac gtacctcccg gttttgccgg
     5881 gagttacatg gcctgaaatt ttgtatttca ttgctacatt cattcaaacc acctgtaaca
     5941 aaaagctgcc tttgacgatc tattgctata aataagtgaa cttttttctt taattgttta
     6001 tttattttaa aatggttaag ttttgctaat catgatgagc tatttttgat agttcatcct
     6061 gtggcgtcgc tcttaaactt atattgtaaa tgacttagat ttaaatgaga taataatttt
     6121 gttaacaata tgaggcggga gatgctttct acaaacttaa tacagagtaa ttatggtgat
     6181 ttaaatatta aaagcctggc tttcgactct tttaaagaaa ggctgcagtc tactatgaca
     6241 gcactcactt tctttatatc aacgggtcaa tgtgactgcg atgaaatagc agaatcaaac
     6301 tttaattata tgattgccta tatgtctaac attaactatg atgcctcaaa gccaggagcg
     6361 ccagcactta gttttgatac atatctgcag gacaatgtta aatatagagt aataattaat
     6421 aatctttatg gtagtgaaat tcggataaga ggtataaata aagattttat tggaatggat
     6481 gtcacttctg tatttcgccc agaaaaaatg accaatttga tcaatataaa aaacaggttg
     6541 gttattcact atttaaggac tatatactat gaacaaaatt atatacatcc tgtcgggagc
     6601 atttttgctg cgattcaaaa aaatgagagt ctcctaaagt ttccttcgat aatatcaatg
     6661 cttaatatca gtcttctttt taatccacta aatcttccgg gaatgggaaa tggtgtgctt
     6721 gaagatatta tgagtatttc agatagttca cttagaaaac gtctcgggta tgaggtttta
     6781 agcttctcgc tacaggcaca ttcattgagc caggaatgta ttgataagtt ggccattttt
     6841 ttcgctgacg atctgtttca atatgagtca gtatgcatcg ccgcgatgga acatttaaaa
     6901 agtaaggcaa cggtccctat acaaaacgga ccgttgccag cataagaatt aacggtgtgc
     6961 cagttcggcg tcgtcttcgc tatccagaat ggctttatcg gtttgcttca gccactggct
     7021 ggtcagcgtg ccagctgtca tcgagccact aacgtttagc gccgtacggc ccatgtcgat
     7081 aagcggttca acggagatta acagcgccac cagggttact ggcaggccca tcgcaggcag
     7141 cacaatcagt gcggcgaaag ttgcaccacc accgacaccg gcaacgcctg cggaactaac
     7201 ggtaacaata ccgaccagcg tcgcaatcca catcgggtcc atcgggttaa tgccaaccgt
     7261 aggcgcaacc atcaccgcca gcatcgccgg atacaaaccg gcacagccgt tctgaccaat
     7321 ggttgcaccg aaagaggcgg cgaaactggc gatggattca ggaacaccga gtcgacgcgt
     7381 ttgtgcttcc acattcagtg ggatagacgc agcactggaa cggctggtaa aggcaaacgt
     7441 cagaactggc cataccttac ggaagtattt cagcgggctc acgccattaa tgcccagcag
     7501 aaggccatgc actgcaaaca taatcagcag accgaggtag gacgcgacaa cgaaacttcc
     7561 cagtttgatg atgtcttgca ggttagaacc tgcaaccact ttggtcatca gcgccagaac
     7621 gccgtaaggg gtcagctgca tgaccaggcg aaccagtttc atcacccagc tttgcagggt
     7681 atcgatagcg gttaagacgc gttcaccttt cggcgcatca tccttcagca gcttgagcgc
     7741 agctacgccg aggaatgcgg caaaaattac cacgctgata attgacgtcg gattggctcc
     7801 ggtcaaatcg gcaaacgggt ttttcgggat aaaggacaag accagctgcg gaacgctcag
     7861 gtcagagact ttaccaacat agttactttc gatggcgttc aaacgtgcag tttctgcacc
     7921 accctgaacc agaccttcag ccgtcaaacc aaacaggttg gtgaccagca caccgaccag
     7981 cgccgcaatc agcgtggtaa acaaaagcgt tccgatggtc agaaaactga ttttgcctaa
     8041 ctgagatgcg ttatgcagac gggcaaccgc gctcagaata gaggcaaaga ctaacggcat
     8101 aacgatcatt tgcagcagtt gaacatagcc gttaccaacg atgttaaacc actgtacaga
     8161 atctttaagt acctggctgt cagaaccata aatggtatgc agggcgaggc caaaaaccac
     8221 acccatcacc agacccacca gcactttttt cgccagactc cactgtttat ggcgggtctg
     8281 agccagcgca aacagcagta caacgaacac cacgatgttc gcaattaatg gaaagttcat
     8341 ccccgttctc ctgatcttat tatttctatc actgcgtagc agtcatagtg ttggcgcaag
     8401 attaacagaa ctgtgagctg gctcttatat tcaaatggaa ttgcttattc cagaaaggtg
     8461 atttttgcct ttttagtatt caacctgatg cttaataagc cgatgaaact ggatctgcaa
     8521 ggtattgatc atttgcgatg accaacggac agcgctgttt tcgggtaatg aatgaggcat
     8581 ccataccgac cagacaaata atgccatgca gataaaacgt tccagttggt tgcccttttg
     8641 gggaaccagg ataggcaagc ggaaacgcca gcggcatggc cagagcaggg gaacaccggc
     8701 gggtgtcagc atatcggcaa gtatgtggct caaataaccc agcaccatgc cctgcaatgc
     8761 atcagccgga atgaaccagc tttcaggaac cttaaggtaa aaggttgcca gcagcgcaaa
     8821 taccgccagc agactgtggg taaaaccacg atgcccaaaa gcgcgggcga tcggttttga
     8881 tatccacttt aatcgctgcc caagaaacga ctttgggtga tcgatgtccg gtaacaaaca
     8941 cgtcaggatt gcggaaggga caatatgcca ccagtcaccc tgtgccagca cgggcgtcag
     9001 ctcggcattt ttggcaaata ccgcacaagc aatagaaaag agaaggtgac cttccgccgt
     9061 catgataaaa ctcactaaac tgtcgatacg tacagtatag gatttttata cagtgcgcgg
     9121 aagtggtgac ggtgtaactc tttctcttaa attgacgatt aatccgtcag ttgaatgcaa
     9181 attcagataa agaaccagga gcagtgtgat cactgcccct ggtcattaac cgagaaggtc
     9241 ttttgcggtg agttctgtca gcgatgagag tttgacgttt gctaatacaa aacgtggatc
     9301 attttgcgct tctggcgcag gaacgacgat ggaacgcatg cgggctgctt tagaagcgat
     9361 catgccattt accgagtctt ccagcgccac gcaggtcagt gggtcaacgc ccagttttgc
     9421 tgcgcagtcg agatatactt gcggatgcgg cttgctgtaa ggcaattttt cagccgaggc
     9481 gagggcatcg aaactgtcgc gtaagtcaaa catggtcaac actttttcca gcatatgtag
     9541 tggtgacgcg gaggccagtc ccaccaataa accttgttct ttgcataacg caacggcttc
     9601 acgcacgcct ggcaataatg ggcgtgtctc ttcaaccagt gaaatggcac gggcaataac
     9661 ccgttctact acttcctgac ggcttggccc attccagggt tgccgggcgt accaaagatc
     9721 gaccaccata tcgatgcgta aacctaaagt gtccggcagc tcgttacgac gggagatatc
     9781 cacccccagg cttgccatca catccagttc agctcgatcc cataaaggtt ctgagtcgat
     9841 aagtaatcca tccatatcaa aaattgcagc aagaatctga cgcggggttg acattacaac
     9901 ctctccttta gagcattatg tgtaaggtat taagccgtgc ttatgattaa gcgcctatca
     9961 tatcgccttt aaagaatggg cgaaaacaga tatctacagg taaactaagt gaaacgacgc
    10021 gtcttaatca aggagaactc atgacgtatc aacaagctgg acgcattgct gttttgaaaa
    10081 ggattttggg ttgggtgatt tttatacctg cattgatctc tacattgatt tctttgctta
    10141 aatttatgaa tacccggcag gaaaaccagg aaggcattaa tgcggtcatg ctcgatttta
    10201 ctcatgtcat gatcgatatg atgcaggcga atacgccttt tttgaatctg ttctggtata
    10261 actccccgac gcccaatttt aatggtggcg tgaacgttat gttctggatg attttcatcc
    10321 tgatttttgt cggactggcg ttgcaggatt ccggtgcccg gatgagtcgc caggcgcgct
    10381 tcttgcggga aggtgttgaa gatcaactca ttctggaaaa agccaaaggg gaagaggggc
    10441 taacgcgtga acaaattgag tcacgtattg ttgtgccgca ccatactatt ttcctgcagt
    10501 ttttctcgct gtatatcctg ccggttattt gtattgctgc aggttatgtg ttcttttctc
    10561 tgctggggtt tatttaatca aaaggccgca cggagcggcc attatcctca ccccatatta
    10621 tgctgctaat aatctatcca gtgactgctg agcaataacc aaatgctgac cgccaaataa
    10681 ccttgcacga tttaacagcg tgtagagttg gtaaaccggt tgacgttcaa ggaaatcggc
    10741 aggtagcggg gatactgact gatagccgtc atagatttgt ggcggctgtt cagtatgcag
    10801 cggtaacatc gccaggtcgc actctcggtc accccagtag caggccgggt cgaaaatgta
    10861 cgggccatcc ggacccagcg cacagttgcc ggaccataaa tcgccgtgca acagagaagg
    10921 ctgcggttga tgcgaggcca gacgctgctg aatatgctcg acgatggcat cgatattgcc
    10981 gaaagcgatc cctttttctg ccgccagttc cagctgccag ccaatccgtt gttcagcaaa
    11041 aaacgttgac cagcgacgtt gccaggtatt gggctgggga gttgtggaga gcgcgttatc
    11101 gaaatcgagg ccaaattgag gttggtcgct ccattgatgt aaacgcgcaa tttgctgacc
    11161 aagaataaat gcgctatgcg catccagcgg gcgaggaggg agatagtcca tcaccagaaa
    11221 actgtagtca cggtcagcgc caactgccca aaccttaggc acagtgacgg ttttactacg
    11281 cgacagtaac tccagttggt cggcttcggc ggtaaaaccg ggaagcagtt ccctttcatc
    11341 acatttgacg aaaaagtcac gtcctgcata gcgcaaatgc catgcggcat ggacttctcc
    11401 gccaggcagt tcattacgca gttcgatttc gccttcacct aactgctcgc tcaaaagacg
    11461 actgattgcc tgccacatga tgtctctccc atgttgtctg ccagatcata aggttagcgc
    11521 aagaatgcgg tgtaaaaaca cgagctaacg cacaccacag actgattatt gcccaaaaac
    11581 catttatttt ctgttgctat tccattcctc ccaggtcgtg atggtgattt caggatcttt
    11641 gccggttgca ctttgggtaa gccccgaaac cagttctcta atttcttctt cactttgggt
    11701 actgataagt ccaaaagtgt tagtccccag ctcatggata tttccatcat catccgtcat
    11761 ggtgagcaaa aaaccgtcgc gtgtcaggta attattcaac tcattaattt cagtcaatgt
    11821 atcctcatgc aacatgacgg ttatgacata acggacaaga tcgccactgg ccatagttca
    11881 cccctttgtt atcacatgac ttttgagcat agtcggagaa acgcgttgcc gacaggttgg
    11941 tggtgattcc cccaatgcag ggggaatgtt tcagttagcg ggaaaggtaa gcgtaaattt
    12001 tttgtgtatc gtcatgggag cacaggcgtg ttccctgatt gagtgtggct gcactccccg
    12061 cagctacgcc aaaacggacc atctcttcaa gagaggcatt ttctgccagt ttcagtgtca
    12121 tcgcgccgac catgctgtca ccagcgccaa cggtgctctg gcttttcacc ggtggtggca
    12181 caacctgaat gcagttttca ctatcaacgc ccaacgcccc ttgtggaccc agggaaacga
    12241 caactcgttt ggccttgccg ctattaacaa tttcctgcgc ggctttgcgg acatcgtctg
    12301 gctgggtgag ttcgcgattc accagcgcac tgagttcttt ttggttaggc ttaaccaact
    12361 cgatgttacc aatcgccagt gctgcactta acgcttcgcc agaactgtcg acgatacagc
    12421 ggatcccttg gttttgcgca gcggaaatca gttgggttaa tttttccagc ttcacacctg
    12481 gcggcaggct tccgcttatg accaggatag caccggattc aatttccaga acttgctctt
    12541 caagctggcg aaactcatct tcatttaatg ccgcgcctgg cataacaaaa cgatactgct
    12601 caccgcttgc ttccacatgt acgtgtaaat tctgccgggt ccagtctttg gcgtctacag
    12661 tagcgacggg gacattttca tccgccaaca gtgaaaccag gtgttcgccg gtcgcaccac
    12721 ccgccgggaa gatcgctgtg gcactgcctc caagatgggc aatggcgcgg gcgacgttga
    12781 tgccgccgcc cccgggttcg aacaccggtg cggtacagcg cagttttcct tcgggataaa
    12841 tttgcggggt aattgttgcg ctatcgagag agggcgcaag tgtcaacgta tagatacgta
    12901 ccatcatttc ctcctatagg ctgatttcag tctggcaccg aatcagcgga aagtgaagaa
    12961 attaacaata tgatttatta gagctttatt taaaattttg cagataaata tatataaata
    13021 aaaatctgtt ttcgccatat gcttattcaa aaaatgaaat aagaatacat tgctaagtta
    13081 ttcgcgcctt tttataattc gttacctttc cagggacgct aatctttgat ccttaagaaa
    13141 gtttccggga ccgcgaaggt ctcttttttt gttgtacgga ctcttgataa atgaagcttt
    13201 tgaagacagt tcctgccatc gttatgttgg cggggggcat gtttgcgtca ctgaatgcag
    13261 ctgccgatga ttccgttttt actgtcatgg acgatcctgc cagcgctaaa aaacctttcg
    13321 aaggcaatct gaacgcagga tatcttgcac aatcaggcaa tacgaaaagc tcctcactta
    13381 cggctgacac caccatgacc tggtatggac aaaccactgc ctggtcgctg tgggggaacg
    13441 ccagtaatac ttcttctaat gatgagcgtt cttcagaaaa atatgcggcg ggcgggcgta
    13501 gccgttttaa tttaactgat tatgactatt tatttgggca ggcaagctgg ctgacagacc
    13561 gttataacgg ctatcgagag cgcgatgtgt tgaccgcggg ttatggtcgc cagtttttga
    13621 atggcccggt acacagtttc cgctttgaat tcggtccagg tgtgcgttac gacaaatata
    13681 ccgacaatgc cagtgaaacc cagccgctgg gttatgcctc cggggcttat gcatggcagt
    13741 tgactgacaa cgctaaattt acgcagggtg tttccgtctt tggcgcggaa gacacaacgc
    13801 tgaactctga aagcgcatta aatgttgcta ttaatgaaca ttttggactg aaggtggctt
    13861 acaacgtcac atggaactct gaacctccag agtctgcacc ggaacatacc gatcgtcgga
    13921 caaccttatc gctgggctac agcatgtaac attatctgcc ggaccgtagt atgcggtctg
    13981 gctgttttcg tttgtcactg ctcagcccca aatcattgct gcccaacgta gcaacaacat
    14041 agcgccacag attgccagta gcaccaccac catcttccag tgttttttat caaagcgttt
    14101 agtcggcata ccagtgtcct gcatatctct tgtgcgtgat aacggagtgt aacaaaaggg
    14161 acaggcatca agggattttt tgttattaca gccgtcgggt ggcgttgtac acctgatcac
    14221 tgtttctgag ttttatgaat aaaataaagt atatgtatca acatatacat cgtaaatatt
    14281 tagttataaa tacgcctgta gttcatctgg actaaagctc aggctttggc tgattacatt
    14341 tatagctgca taatatgttt ctacagtttt ataatgatta tgatgaatgg ctatatgaac
    14401 agctgacatg ttgtcatgat ttttagcggc caacaatgtg aataactgac attgactaaa
    14461 agaatatttt tttgcaaaag tacccagcgt tgatatataa gataacacga cgtctttatt
    14521 acccttgctc atggcgatgt ataaagcagg ggttccatgt gctgttgcgc cttttaataa
    14581 atccatgatg ttaattttgc tgaggttata tttaacagcg attccataaa ctttagagag
    14641 gaaccgtgtg acacacaaag ggtgattatt ttccattgcg gcatataatc ctggctcacc
    14701 atcacgtgtt ttcattgcta aaatattcat taccattttt ttgtttaaaa gatcttgtgc
    14761 tattggagtg gttaatgcat tcagaaagat atcgattatt tcatcgaatc catacaacat
    14821 ggagatatag agtccaggtg tttcgttagc acttttagtt tgcagtaatt taaccagatt
    14881 atcttcatga atgatatgat tatctaccag tgactgaatt tcttgcataa atatttttac
    14941 gttgtcagta tgtccgttca tcatagccag aaataaagca ggtgtaccaa cgctattctg
    15001 cgcagttatt atttcattga tttctttttc agggagtatg ggtaactgtt tgaataatgt
    15061 aataagctgt tgagtctggc ctgtggccat tattaacaac attgccgatg gatgtatagg
    15121 ggcaaatggc attcgtgatg caatacccgc gaaaatagcc cctggaactc cctcaggtaa
    15181 taggggcaga aaagcaattg cacagttgcg aacatacata tcagcccagg tctgagcatc
    15241 gggacctgaa ctgatgaatt tatcgagcga gagaaagccc cgtgctgtcc tgtgggtgcc
    15301 tctgacggca acgttggtat cgttcgggtc gtaaacggac acaacataat ttaattgccc
    15361 atacgctgac tgctttattc gtagccttac tgacaaagta tgattgtcta tatataaaat
    15421 cgcggacata tgactgacat tgtttgccat cattcgacag aagaaaatat ctaaagcgaa
    15481 gccaaaatcc ttactcgcga taacgcgact ccctgcagga ggatggcgaa gtaatgatct
    15541 gaactccgcg tcccggacta agggaacatg ctgctgtatg gattcaacgg aggagtaatg
    15601 tttaatcaga ttgactttgc cttttccatt tctcagtgaa tcaagggcgt attgcaatga
    15661 cagatggcga caaaaaatag cgtcagaagg agattgcaaa aaaaatgcac taccattgag
    15721 atttactgtt ggttgtatag ttttttctct atggattttt ttagacagtt tcatacaatg
    15781 gttacgattt tcggcagtta actgttcctg ttcaatataa tccagtatgc ttcgcaaatg
    15841 gttagcttta ataggcgtgg tttccagtaa taaggaaatg atttcttttt tatagtgtaa
    15901 tgtaatgagt atattttctg catggatggg tgaagtggca aagttaatgg taaaaagggc
    15961 agtggaataa caattttgtg atatttttcg acagtttgcg gtaaattcct gagtaacaca
    16021 gtatgtcagc atcgaactta agtcagaggt atcaagtagg gtgatgagtt ggcgcgcaaa
    16081 ttgattaata ggctctgatg aaggcgttgg tatgtcgcta tcgatgtgca ttctatgagg
    16141 agacttataa tgagttctga taataatgtc gttttgactc ataatctatt cgccttatat
    16201 atgtctggag gttttgagga tgatgattaa ttattatcgc cagaacgaaa tgtgcacgaa
    16261 aaattgctat aagaattgtg tcacttttat cattgtgata tgaccacttc gtcattaatc
    16321 ttatgatcgt ttaaatagta ccgccaaatc acgcaacgaa ctttgctgga ttcagtttat
    16381 gctgctgcaa atccgctcgc agtaaacctt tcagacgctc cgtgatgtta tcagtggttc
    16441 tttaagcgtt ttgctggtgt actcactaca aacgaattgc gaatcgaatc aatgtgaaac
    16501 ggaaaggtac aatctccctt tttttagtgt taacatcgtt caaccgggtg gaaggtgaga
    16561 ggttaatgtc tttcatttgt gtacctgatt gcgaaccaat ttagcatttg ttggctagat
    16621 ggtttcgcaa tgaactgtta ataaacaaat ttttctttgt atgtgatctt tcgtgtgggt
    16681 caccactgca aataaggata taaaatgcct gttataactc ttcctgatgg cagccaacgc
    16741 cattacgacc acgctgtaag ccccatggat gttgcgctgg acattggtcc aggtctggcg
    16801 aaagcctgta tcgcagggcg cgttaatggc gaactggttg atgcttgcga tctgattgaa
    16861 aacgacgcac aactgtcgat cattaccgcc aaagacgaag aaggtctgga gatcattcgt
    16921 cactcctgtg cgcacctgtt agggcacgcg attaaacaac tttggccgca taccaaaatg
    16981 gcaatcggcc cggttattga caacggtttt tattacgacg ttgatcttga ccgcacgtta
    17041 acccaggaag atgtcgaagc actcgagaag cggatgcatg agcttgctga gaaaaactac
    17101 gacgtcatta agaagaaagt cagctggcac gaagcgcgtg aaactttcgc caaccgtggg
    17161 gagagctaca aagtctccat tcttgacgaa aacatcgccc atgatgacaa gccaggtctg
    17221 tacttccatg aagaatatgt cgatatgtgc cgcggtccgc acgtaccgaa catgcgtttc
    17281 tgccatcatt tcaaactaat gaaaacggca ggggcttact ggcgtggcga cagcaacaac
    17341 aaaatgttgc aacgtattta cggtacggcg tgggcagaca aaaaagcgct taacgcttac
    17401 ctgcagcgcc tggaagaagc cgcgaaacgc gaccaccgta aaatcggtaa acagctcgac
    17461 ctgtaccata tgcaggaaga agcaccgggt atggtattct ggcacaacga tggctggacc
    17521 atcttccgtg aactggaagt gtttgttcgt tctaaactga aagagtacca gtatcaggaa
    17581 gttaaaggtc cgttcatgat ggaccgtgtc ctgtgggaaa aaaccggtca ctgggacaac
    17641 tacaaagatg caatgttcac cacgtcttct gagaaccgtg aatactgcat taagccgatg
    17701 aactgcccgg gtcacgtaca aattttcaac caggggctga agtcgtatcg cgatctgccg
    17761 ctgcgtatgg ccgagtttgg tagctgccac cgtaatgagc cgtcaggttc gctgcatggc
    17821 ctgatgcgcg tgcgtggatt tacccaggat gacgcgcata tcttctgtac tgaagaacaa
    17881 attcgcgatg aagttaacgg atgtatccgt ttagtctatg atatgtacag cacttttggc
    17941 ttcgagaaga tcgtcgtcaa actctccact cgtcctgaaa aacgtattgg cagcgacgaa
    18001 atgtgggatc gtgctgaggc ggacctggcg gttgcgctgg aagaaaacaa catcccgttt
    18061 gaatatcaac tgggtgaagg cgctttctac ggtccgaaaa ttgaatttac cctgtatgac
    18121 tgcctcgatc gtgcatggca gtgcggtaca gtacagctgg acttctcctt gccgtctcgt
    18181 ctgagcgcct cctatgtggg cgaagacaac gagcgtaagg taccggtaat gattcaccgc
    18241 gcaattctgg ggtcgatgga acgtttcatc ggtatcctga ccgaagaatt cgctggtttc
    18301 ttcccgacct ggcttgcgcc ggttcaggtt gttatcatga atattaccga ttcacagtct
    18361 gaatacgtta acgaattgac gcaaaaacta tcaaatgcgg gcattcgtgt taaagcagac
    18421 ttgagaaatg agaagattgg ctttaaaatc cgcgagcaca ctttgcgtcg cgtcccgtat
    18481 atgctggtct gtggtgataa agaggtggaa tcaggcaaag ttgccgttcg cacccgccgt
    18541 ggtaaagacc tgggaagcat ggacgtaaat gaagtgatcg agaagctgca acaagagatt
    18601 cgcagccgca gtcttaaaca attggaggaa taaggtatta aaggcggaaa acgagttcaa
    18661 acggcgcgcc ctaaccgtat caatggcgaa attcgcgccc aggaagttcg cttaacaggt
    18721 ctggaaggcg agcagcttgg tattgtgagt ctgagagaag ctctggagaa agcagaagaa
    18781 gccggagtag acttagtcga gatcagccct aacgccgagc cgccggtttg tcgtataatg
    18841 gattacggca aattcctcta tgaaaagagc aagtcttcta aggaacagaa gaaaaagcaa
    18901 aaagttatcc aggttaagga aattaaattc cgtcctggta cagatgaagg cgactatcag
    18961 gtaaaactcc gcagcctgat tcgctttctc gaagagggtg ataaagccaa aatcacgctg
    19021 cgtttccgcg gtcgtgagat ggcgcaccag caaatcggta tggaagtgct taatcgcgtg
    19081 aaagacgatt tgcaagaact ggcagtggtc gaatccttcc caacgaagat cgaaggccgc
    19141 cagatgatca tggtgctcgc tcctaagaag aaacagtaag gccttcaagt agcaatgtct
    19201 gtggagcctt cgggcttcac aggctttgtt cgcctttgtt tcgtttatta acaatgcgaa
    19261 gtggaagtta ttaaaatgcc aaaaattaag accgtacgcg gtgctgctaa gcgcttcaaa
    19321 aaaaccggta aaggtggttt taagcacaag cacgctaacc tgcgtcacat tctgaccaaa
    19381 aaagcgacca aacgtaaacg tcacctgcgt ccgaaagcca tggtttccaa aggcgatctg
    19441 ggcctggtaa tcgcgtgcct gccgtacgca taagccgtta acgtttttta actttttaat
    19501 tagaatatag atacaggaga gcacatatgg ctcgcgtaaa acgtggtgtt attgcacgcg
    19561 cacgtcacaa gaaaattttg aaacaagcta aaggctacta cggtgcgcgt tctcgcgtat
    19621 accgcgttgc cttccaggct gttatcaaag ctggtcagta tgcttaccgt gaccgtcgtc
    19681 aacgtaagcg tcagttccgt caactgtgga ttgcgcgtat caacgcagca gcacgtcaga
    19741 acggtatttc ttacagcaaa ttcatcaatg gcctgaaaaa agcctctgtt gaaatcgacc
    19801 gtaagatcct ggctgatatc gcagtattcg acaaagtagc gttcaccgct ctggttgaaa
    19861 aagcgaaagc agctctggca taagccagtt gaaagaggga gctagtctcc ctcttttcgt
    19921 ttcaacgcca tcaaaacatt gacttttatc gccgtagcct tttcaataaa ggtcttttga
    19981 aaagtaccaa aaggtaacgc aagcaatgaa tgctgctatt ttccgcttct ttttttactt
    20041 tagcacctga ctccaggagg ctagcgcgtg agaagagaaa cggaaaacag cgcctgaaag
    20101 cctcccagtg gaggcttttt ttgtatgcgc gttttgaaaa aattaagtcc aacgaaccag
    20161 tgtcaccact gacacaatga ggaaaaccat gtcacatctc gcagaactgg ttgccagtgc
    20221 aaaggcggcc attagccagg cgtcagatgt tgccgcgtta gacaatgtgc gcgtcgaata
    20281 tttgggtaaa aaagggcact taacccttca gatgacgacc ctacgtgagc tgccgccaga
    20341 agagcgtccg gcagctggtg cggttatcaa tgaagcgaaa gagcaggttc agcaggcgct
    20401 gaatgcgcgt aaagcggaac tggaaagcgc tgcactgaat gcgcgtctgg cggcggaaac
    20461 gattgatgtc tccctgccgg gtcgtcgcat tgaaaacggc gggctgcatc cggtgacccg
    20521 taccatcgac cgtatcgaaa gtttcttcgg tgagcttggc tttaccgtgg caaccgggcc
    20581 ggaaatcgaa gacgattatc ataacttcga tgctctgaac attcctggtc accacccggc
    20641 gcgcgctgac cacgacactt tctggtttga cgctacccgc ctgctgcgta cccagacctc
    20701 tggcgtacag atccgcacca tgaaagctca gcagccaccg attcgtatca tcgcgcctgg
    20761 ccgcgtttat cgtaacgact acgaccagac tcacacgccg atgttccatc agatggaagg
    20821 tctgattgtt gataccaaca tcagctttac caacctgaaa ggcacgctgc acgacttcct
    20881 gcgtaacttc tttgaggaag atttgcagat ccgcttccgt ccttcctact tcccgtttac
    20941 cgaaccttct gcagaagtgg acgtcatggg taaaaacggt aaatggctgg aagtactggg
    21001 ctgcgggatg gtgcatccga acgtgctgcg taatgttggc atcgacccgg aagtttactc
    21061 tggtttcgcc ttcggaatgg ggatggagcg tctgaccatg ttgcgttacg gcgtcaccga
    21121 cctgcgttca ttcttcgaaa acgatctgcg tttcctcaaa cagtttaaat aaggcaggaa
    21181 tagattatga aattcagtga actgtggtta cgcgaatggg tgaacccggc gattgatagc
    21241 gatgcgctgg caaatcaaat cactatggcg ggcctggaag ttgacggtgt agaaccggtt
    21301 gccggtagct tccacggcgt ggttgtcggt gaagtggttg aatgtgcgca gcatccgaac
    21361 gctgacaaac tgcgtgtgac aaaagtgaat gtcggcggcg atcgcctgct ggacatcgtc
    21421 tgcggtgcgc caaactgccg tcagggcctg cgtgtggcgg tagcgaccat tggtgctgtt
    21481 ctgccgggcg atttcaaaat taaagcggcg aaactgcgtg gcgaaccgtc tgaagggatg
    21541 ctgtgctctt tctctgagct gggaatttct gacgatcaca gcggtattat cgaactgcct
    21601 gcggatgcgc caattggcac cgacattcgt gaatacctga aactcgatga caacaccatc
    21661 gaaattagcg taacgccaaa ccgtgccgac tgcttaggta tcattggtgt tgcgcgtgac
    21721 gttgccgtgc tgaaccagct gccgctggtt gaaccggaaa tcgttccggt tggtgcgacc
    21781 atcgacgaca cgctgccgat tgcagtcgaa gcgcctgaag cttgtccgcg ctatcttggc
    21841 cgtgtggtaa aaggcattaa cgttaaagcg ccaactccgt tgtggatgaa agaaaaactg
    21901 cgtcgttgcg ggatccgttc tatcgatgca gttgttgacg tcaccaacta tgtgctgctc
    21961 gaactgggcc agccgatgca cgctttcgat aaagatcgca ttgaaggcgg cattgtggtg
    22021 cggatggcga aagagggcga aacgctggta ctgctcgacg gtaccgaagc gaagctgaac
    22081 gctgacaccc tggtcatcgc cgaccacaac aaggcgctgg cgatgggcgg catctttggt
    22141 ggcgaacact ctggtgtgaa tgacgaaacg caaaacgttc tgctggaatg tgctttcttc
    22201 agcccgctat caatcaccgg tcgtgctcgt cgtcatggcc tgcatactga tgcgtctcac
    22261 cgttatgagc gtggcgttga tccggcactg cagcacaaag cgatggaacg tgcgacccgt
    22321 ctgctgatcg acatctgcgg tggtgaggct ggtccggtaa ttgatatcac caacgaagca
    22381 acgctgccga agcgtgcaac catcactttg cgtcgcagca aactggatcg cctgattggc
    22441 catcatattg ccgatgagca ggtcactgac attctgcgtc gtctcggctg cgaagtgacc
    22501 gaaggcaaag acgaatggca ggccgttgcg ccgagctggc gtttcgacat ggagattgaa
    22561 gaagatctgg ttgaagaagt ggcgcgtgtt tacggctaca acaacatccc ggatgagccg
    22621 gtacaggcaa gcctgattat gggtactcac cgtgaagctg acctgtcgct caagcgcgtg
    22681 aaaacgctgc tcaacgacaa aggctatcag gaagtgatca cctacagctt cgttgatccg
    22741 aaagtgcagc agatgatcca tccaggcgtt gaagccttac tgctgccaag cccgatctct
    22801 gttgaaatgt cagcaatgcg tctttctctg tggaccggcc tgctggcaac cgtggtgtac
    22861 aaccagaacc gtcagcagaa ccgtgtgcgc attttcgaaa gcggtctgcg tttcgtacca
    22921 gatactcagg caccgttggg cattcgtcag gatctgatgt tagccggtgt gatttgcggt
    22981 aaccgttacg aagagcactg gaacctggca aaagagaccg ttgatttcta tgatttgaaa
    23041 ggcgatcttg aatccgttct cgacctgacc ggtaaactga atgaggttga gttccgtgca
    23101 gaagctaatc cggcactgca tccggggcaa tccgcagcga tttatctgaa aggtgaacgt
    23161 attggttttg ttggggttgt tcatcctgaa ctggaacgta aactggatct taacggtcgc
    23221 actctggtgt tcgaactgga gtggaacaag ctcgcagacc gcgtggtgcc tcaggcgcgc
    23281 gagatttctc
//
LOCUS       DAMZFT010000077        23241 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.077, whole genome shotgun sequence.
ACCESSION   DAMZFT010000077 DAMZFT010000000
VERSION     DAMZFT010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 23241)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23241)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23241
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.077"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(141..2684)
                     /gene="mdoH"
                     /locus_tag="PN977_003079"
     CDS             complement(141..2684)
                     /gene="mdoH"
                     /locus_tag="PN977_003079"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis glucosyltransferase MdoH"
                     /protein_id="HDI6043091.1"
                     /translation="MNKTTEYIDAMPIAASEKAALPKTDIRAVHQALDAEHRTWARED
                     DSPQGSVKARLEQAWPDSLADGQLIKDDEGRDQLKAMPEAKRSSMFPDPWRTNPVGRF
                     WDRLRGRDVTPRYLARLTKEEQESEQKWRTVGTIRRYILLILTLAQTVVATWYMKTIL
                     PYQGWALINPMDMVGQDVWVSFMQLLPYMLQTGILILFAVLFCWVSAGFWTALMGFLQ
                     LLIGRDKYSISASTVGDEPLNPEHRTALIMPICNEDVNRVFAGLRATWESVKATGNAK
                     HFDVYILSDSYNPDICVAEQKAWMELIAEVGGEGQIFYRRRRRRVKRKSGNIDDFCRR
                     WGSQYSYMVVLDADSVMTGDCLCGLVRLMEANPNAGIIQSSPKASGMDTLYARCQQFA
                     TRVYGPLFTAGLHFWQLGESHYWGHNAIIRVKPFIEHCALAPLPGEGSFAGSILSHDF
                     VEAALMRRAGWGVWIAYDLPGSYEELPPNLLDELKRDRRWCHGNLMNFRLFLVKGMHP
                     VHRAVFLTGVMSYLSAPLWFMFLALSTALQVVHALTEPQYFLQPRQLFPVWPQWRPEL
                     AIALFASTMVLLFLPKLLSILLIWCKGTKEYGGFWRVTLSLLLEVLFSVLLAPVRMLF
                     HTVFVVSAFLGWEVVWNSPQRDDDSTSWGEAFKRHGSQLLLGLVWAVGMAWLDLRFLF
                     WLAPIVFSLILSPFVSVISSRATVGLRTKRWKLFLIPEEYSPPQVLVDTDRFLEMNRQ
                     RSLDDGFMHAVFNPSFNALATAMATARHRASKVLEIARDRHVEQALNETPEKLNRDRR
                     LVLLSDPVTMARLHFRVWNSPERYSSWVSYYEGIKLNPLALRKPDAASQ"
     gene            complement(2677..4212)
                     /gene="mdoG"
                     /locus_tag="PN977_003080"
     CDS             complement(2677..4212)
                     /gene="mdoG"
                     /locus_tag="PN977_003080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoG"
                     /protein_id="HDI6043092.1"
                     /translation="MMKMRWLSAAVMLTLYTSSSWAFSIDDVAKQAQSLAGKGYEAPK
                     SNLPSVFRDMKYADYQQIQFNHDKAYWNNLKTPFKLEFYHQGMYFDTPVKINEVTATA
                     VKRIKYSPDYFTFGDVQHDKDTVKDLGFAGFKVLYPINSKDKNDEIVSMLGASYFRVI
                     GAGQVYGLSARGLAIDTALPSGEEFPRFKEFWIERPKPTDKRLTIYALLDSPRATGAY
                     KFVVMPGRDTVVDVQSKIYLRDKVGKLGVAPLTSMFLFGPNQPSPANNYRPELHDSNG
                     LSIHAGNGEWIWRPLNNPKHLAVSSFSMENPQGFGLLQRGRDFSRFEDLDDRYDLRPS
                     AWVTPKGEWGKGSVELVEIPTNDETNDNIVAYWTPDQLPEPGKEMNFKYTITFSRDED
                     KLHAPDNAWVQQTRRSTGDVKQSNLIRQPDGTIAFVVDFTGAEMKKLPEDTPVTAQTS
                     IGDNGEIVESTVRYNPVTKGWRLVMRVKVKDAKKTTEMRAALVNADQTLSETWSYQLP
                     ANE"
     gene            4606..5763
                     /gene="mdoC"
                     /locus_tag="PN977_003081"
     CDS             4606..5763
                     /gene="mdoC"
                     /locus_tag="PN977_003081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoC"
                     /protein_id="HDI6043093.1"
                     /translation="MNPVPAQREYFLDSIRAWLMLLGIPFHISLIYSSHTWHVNSAEP
                     SLWLTLFNDFIHSFRMQVFFVISGYFSYMLFLRYPLKKWWKVRVKRVGIPMLTAIPLL
                     TLPQFIMLQYVKGKAESWPGLSLYDKYNTLAWELISHLWFLLVLVVMTTLCVWIFKRI
                     RNNLENSDKTNKKFSMVKLSVIFLCLGIGYAVIRRTIFIVYPPILSNGMFNFIVMQTL
                     FYLPFFILGALAFIFPHLKALFTTPSRGCTLAAALAFVAYLLNQRYGSGDAWMYETES
                     VITMVLGLWMVNVVFSFGHRLLNFQSARVTYFVNASLFIYLVHHPLTLFFGAYITPHI
                     TSNWLGFLCGLIFVVGIAIILYEIHLRIPLLKFLFSGKPVVKRENDKAPAR"
     gene            complement(5771..7252)
                     /gene="clsC"
                     /locus_tag="PN977_003082"
     CDS             complement(5771..7252)
                     /gene="clsC"
                     /locus_tag="PN977_003082"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309451.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsC"
                     /protein_id="HDI6043094.1"
                     /translation="MMKKTPTSTKDSLLNKEMNDLPRLASAVLPLCSQHPGQCGLFPL
                     EKSLDAFAARYRLAEMAEHTLDVQYYIWQDDMSGRLLFSALLAAAKRGVRVRLLLDDN
                     NTPGLDDILRLLDSHPRIEVRLFNPFSFRLLRPLGYITDFSRLNRRMHNKSFTVDGVV
                     TLVGGRNIGDAYFGAGEEPLFSDLDVMAIGPVVEDVADDFARYWYCKSVSPLQQVLDV
                     TEGEMADRIELPASWHNDAMTHRYLRKMESSPFINHLVDGTLPLIWAKTRLLSDDPAK
                     GEGKAKRHSLLPQRLFDIMGSPSERIDIISSYFVPTRAGVAQLLRMVRKGVKIAILTN
                     SLAANDVAVVHAGYARWRKKLLRYGVELYELKPTREQSSTLHDRGITGNSGASLHAKT
                     FSIDGKTVFIGSFNFDPRSTLLNTEMGFVIESETLAQLIDKRFIQSQYDAAWQLRLDR
                     WGRINWVDRHAKKEIVLKKEPATSFWKRVMVRLASILPVEWLL"
     gene            complement(7194..7727)
                     /gene="ymdB"
                     /locus_tag="PN977_003083"
     CDS             complement(7194..7727)
                     /gene="ymdB"
                     /locus_tag="PN977_003083"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HDI6043095.1"
                     /translation="MKTRIHVVQGDITKLAVDVIVNAANPSLMGGGGVDGAIHRAAGP
                     ALLDACLKVRQQQGDCPTGHAVITLAGDLPAKAVVHTVGPVWRGGEQNEDQLLQDAYL
                     NSLRLVAANSYTSVAFPAISTGVYGYPRAAAAEIAVKTVSEFITRHALPEQVYFVCYD
                     EENAHLYERLLTQQGDE"
     gene            complement(7822..8133)
                     /locus_tag="PN977_003084"
     CDS             complement(7822..8133)
                     /locus_tag="PN977_003084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HDI6043096.1"
                     /translation="MFRSFLDSLMLGSMFFPFIAIAGSTAQGGVIHFYGQIVEPACDV
                     STQSTPVEMNCPQNGSVPGRTYSSKALMSGNVKNAQIASVKVQYLDKQKKLAVMNIEY
                     N"
     gene            complement(8254..8586)
                     /gene="csgC"
                     /locus_tag="PN977_003085"
     CDS             complement(8254..8586)
                     /gene="csgC"
                     /locus_tag="PN977_003085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli assembly protein CsgC"
                     /protein_id="HDI6043097.1"
                     /translation="MNALLLLAALSSQITFNTTQQGDMYTIIPEVTLTQSCLCRVQIL
                     SLREGSSGQSQTKQEKTLSLPANQPIALTKLSLNISPDDRVKIVVTVSDGQSLHLSQQ
                     WPPSSGKS"
     gene            complement(8644..9099)
                     /gene="csgA"
                     /locus_tag="PN977_003086"
     CDS             complement(8644..9099)
                     /gene="csgA"
                     /locus_tag="PN977_003086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curlin major subunit CsgA"
                     /protein_id="HDI6043098.1"
                     /translation="MKLLKVAAIAAIVFSGSALAGVVPQYGGGGNHGGGGNNSGPNSE
                     LNIYQYGGGNSALALQADARNSDLTITQHGGGNGADVGQGSDDSSIDLTQRGFGNSAT
                     LDQWNGKDSTMTVKQFGGGNGAAVDQTASNSSVNVTQVGFGNNATAHQY"
     gene            complement(9140..9595)
                     /gene="csgB"
                     /locus_tag="PN977_003087"
     CDS             complement(9140..9595)
                     /gene="csgB"
                     /locus_tag="PN977_003087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curlin minor subunit CsgB"
                     /protein_id="HDI6043099.1"
                     /translation="MKNKLLFMMLTILGAPGIAAAAGYDLANSEYNFAVNELSKSSFN
                     QAAIIGQAGTNNSAQLRQGGSKLLAVVAQEGSSNRAKIDQTGDYNLAYIDQAGNANDA
                     SISQGAYGNTAMIIQKGSGNKANITQYGTQKTAIVVQRQSQMAIRVTQR"
     gene            10350..11000
                     /gene="csgD"
                     /locus_tag="PN977_003088"
     CDS             10350..11000
                     /gene="csgD"
                     /locus_tag="PN977_003088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator CsgD"
                     /protein_id="HDI6043100.1"
                     /translation="MFNEVHSIHGHTLLLITKPSLQATALLQHLKQSLAITGKLHNIQ
                     RSLDDISSGSIILLDMMEADKKLIHYWQDTLSRKNNNIKILLLNTPEDYPYRDIENWP
                     HINGVFYAMEDQERVVNGLQGVLRGECYFTQKLASYLITHSGNYRYNSTESALLTHRE
                     KEILNKLRIGASNNEIARSLFISENTVKTHLYNLFKKIAVKNRTQAVSWANDNLRR"
     gene            11005..11394
                     /gene="csgE"
                     /locus_tag="PN977_003089"
     CDS             11005..11394
                     /gene="csgE"
                     /locus_tag="PN977_003089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgE"
                     /protein_id="HDI6043101.1"
                     /translation="MKRYLRWIVAAEFLFAAGNLHAVEVEVPGLLTDHTVSSIGHDFY
                     RAFSDKWESDYTGNLTINERPSARWGSWITITVNQDVIFQTFLFPLKRDFEKTVVFAL
                     IQTEEALNRRQINQALLSTGDLAHDEF"
     gene            11419..11835
                     /gene="csgF"
                     /locus_tag="PN977_003090"
     CDS             11419..11835
                     /gene="csgF"
                     /locus_tag="PN977_003090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgF"
                     /protein_id="HDI6043102.1"
                     /translation="MRVKHAVVLLMLISPLSWAGTMTFQFRNPNFGGNPNNGAFLLNS
                     AQAQNSYKDPSYNDDFGIETPSALDNFTQAIQSQILGGLLSNINTGKPGRMVTNDYIV
                     DIANRDGQLQLNVTDRKTGQTSTIQVSGLQNNSTDF"
     gene            11862..12695
                     /gene="csgG"
                     /locus_tag="PN977_003091"
     CDS             11862..12695
                     /gene="csgG"
                     /locus_tag="PN977_003091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgG"
                     /protein_id="HDI6043103.1"
                     /translation="MQRLFLLVAVMLLSGCLTAPPKEAARPTLMPRAQSYKDLTHLPA
                     PTGKIFVSVYNIQDETGQFKPYPASNFSTAVPQSATAMLVTALKDSRWFIPLERQGLQ
                     NLLNERKIIRAAQENGTVAINNRIPLQSLTAANIMVEGSIIGYESNVKSGGVGARYFG
                     IGADTQYQLDQIAVNLRVVNVSTGEILSSVNTSKTILSYEVQAGVFRFIDYQRLLEGE
                     VGYTSNEPVMLCLMSAIETGVIFLINDGIDRGLWDLQNKAERQNDILVKYRHMSVPPE
                     S"
     gene            complement(12759..13250)
                     /locus_tag="PN977_003092"
     CDS             complement(12759..13250)
                     /locus_tag="PN977_003092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HDI6043104.1"
                     /translation="MNILLSIAITTGILSGIWGWVAVSLGLLSWAGFLGCTAYFACPQ
                     GGLKGLAISAATLLSGVVWAMVIIYGSALAPHLEILGYVITGIVAFLMCIQAKQLLLS
                     FVPGTFIGACATFAGQGDWKLVLPSLALGLIFGYAMKNSGLWLAARSAKTAHREQEIK
                     NKA"
     gene            complement(13352..13906)
                     /gene="ycdY"
                     /locus_tag="PN977_003093"
     CDS             complement(13352..13906)
                     /gene="ycdY"
                     /locus_tag="PN977_003093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone YcdY"
                     /protein_id="HDI6043105.1"
                     /translation="MNEFSILCRVLGSLYYRQPQDPLLVPLFTLIREGKLAANWPLEQ
                     DELLTRLQKSCDMTQVSADYNALFIGDECAVPPYRSAWVEGATEAEVRAFLSERGMPL
                     ADTPADHIGTLLLAASWLEDQSTEDESEALETLFSEYLLPWCGAFLGKVEAHATTPFW
                     RTMAPLTRDAISAMWEELEEDPEE"
     gene            complement(13930..14667)
                     /gene="ycdX"
                     /locus_tag="PN977_003094"
     CDS             complement(13930..14667)
                     /gene="ycdX"
                     /locus_tag="PN977_003094"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding phosphatase"
                     /protein_id="HDI6043106.1"
                     /translation="MYPVDLHMHTVASTHAYSTLSDYIAQAKQKGIKLFAITDHGPDM
                     EDAPHHWHFINMRIWPRVVDGVGILRGIEANIKNVDGEIDCSGKMFDSLDLIIAGFHE
                     PVFAPHDKATNTQAMIATIASGNVHIISHPGNPKYEIDVKAVAEAAAKHQVALEINNS
                     SFLHSRKGSEDNCRAVAAAVRDAGGWVALGSDSHTAFTMGEFEECLKILDAVDFPPER
                     ILNVSPRRLLNFLESRGMAPIAEFADL"
     gene            complement(14722..15660)
                     /gene="ghrA"
                     /locus_tag="PN977_003095"
     CDS             complement(14722..15660)
                     /gene="ghrA"
                     /locus_tag="PN977_003095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415551.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrA"
                     /protein_id="HDI6043107.1"
                     /translation="MDIIFYHPTFDTQWWIEALRKAIPQARVRAWKSGDNVSADYALV
                     WHPPVEMLAGRDLKAVFALGAGVDSILSKLQAHPEMLKPSVPLFRLEDTGMGEQMQEY
                     AVSQVLHWFRRFDDYRIQQNSSHWQPLPEYHREDFTIGILGAGVLGSKVAQSLQTWRF
                     PLRCWSRTRKSWPGVQSFAGREELSAFLSQCRVLINLLPNTPETVGIINQQLLEKLPD
                     GAYLLNLARGVHVVEDDLLAALDSGKVKGAMLDVFNREPLPPESPLWQHPRVTITPHV
                     AAITRPAEAVDYISRTIAQLEKGERVCGQVDRARGY"
     gene            15894..15981
                     /locus_tag="PN977_003096"
     tRNA            15894..15981
                     /locus_tag="PN977_003096"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:15928..15930,aa:Ser,seq:gga)
     gene            complement(16073..16759)
                     /locus_tag="PN977_003097"
     CDS             complement(16073..16759)
                     /locus_tag="PN977_003097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016232851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2726 domain-containing protein"
                     /protein_id="HDI6043108.1"
                     /translation="MSFMVFVTCLTIAFVAGFAVARKLTLRKVASSVIITKSQPVPSS
                     ATSPAVKASTRSYTPQKTYRDSRWQERDELFQGMVAEGILLEGKEWLITRCEKIYFDN
                     LEKWFGHYCHIHCQVSLGRLINFPEQPEFSEEERKRFFAIYNGMAMDYVLVSKKNNKI
                     VCVIELDDPSHERADRIIRDRWLNTIMTIARVPFVRASVTKLDEIPPVWDTRDKMRPL
                     AAATKGEPLH"
     gene            complement(17322..18110)
                     /gene="phoH"
                     /locus_tag="PN977_003098"
     CDS             complement(17322..18110)
                     /gene="phoH"
                     /locus_tag="PN977_003098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PhoH"
                     /protein_id="HDI6043109.1"
                     /translation="MGRQKAVIKARREAKRVLRRDSRSHKQREEESVTSLVQMSGVEA
                     IGMARDSRDTSPILARNEAQLHYLKAIESKQLIFATGEAGCGKTWISAAKAAEALIHK
                     DVDRIIVTRPVLQADEDLGFLPGDIAEKFAPYFRPVYDVLVRRLGASFMQYCLRPEIG
                     KVEIAPFAYMRGRTFENAVVILDEAQNVTAAQMKMFLTRLGENVTVIVNGDITQCDLP
                     RGVRSGLSDALERFEEDEMVGIVRFGKEDCVRSALCQRTLHAYS"
     gene            complement(18731..20002)
                     /gene="efeB"
                     /locus_tag="PN977_003099"
     CDS             complement(18731..20002)
                     /gene="efeB"
                     /locus_tag="PN977_003099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deferrochelatase/peroxidase EfeB"
                     /protein_id="HDI6043110.1"
                     /translation="MQYEDENGVNEPSRRRLLKGIGALALAGSCPVAHAQKTQSAPGT
                     LSPDARSEAQPFYGEHQAGILTPQQAAMMLVAFDVLASDKTDLERLFRLLTQRFAFLT
                     QGGAAPETPNPRLPPLDSGILGGYIASDNLTITLSVGHSLFDERFGLTPQMPKKLQKM
                     TRFPNDSLDAALCHGDVLLQICANTQDTVIHALRDIIKHTPDLLSVRWKREGFISDHA
                     ARSKGKETPINLLGFKDGTANPDSQNAKLMQKVVWVTADQQEPAWTIGGSYQAVRLIQ
                     FRVEFWDRTPLKEQQTIFGRDKQTGAPLGMQHEHDVPDYASDPEGKVIALDSHIRLAN
                     PRTPESESSLMLRRGYSYSLGVTNSGQLDMGLLFVCYQHDLEKGFLTVQKRLNGEALE
                     EYVKPIGGGYFFALPGVKDANDYLGSALLRV"
     gene            complement(20008..21135)
                     /gene="efeO"
                     /locus_tag="PN977_003100"
     CDS             complement(20008..21135)
                     /gene="efeO"
                     /locus_tag="PN977_003100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake system protein EfeO"
                     /protein_id="HDI6043111.1"
                     /translation="MTINFRRNALQLSVAALFSSAFMANAADIPQVKVTVTDKQCEPM
                     TITVNAGKTQFIIQNHSQKALEWEILKGVMVVEERENIAPGFSQKMTANLQPGEYDMT
                     CGLLTNPKGKLIVKGEATADAAQSDALLSLGGAITAYKAYVMAETTQLVTDTKAFTDA
                     IKAGDIEKAKALYAPTRQHYERIEPIAELFSDLDGSIDAREDDYEQKAADPKFTGFHR
                     LEKALFGDNTTKGMDKYADQLYTDVVDLQKRISELAFPPSKVVGGAAGLIEEVAASKI
                     SGEEDRYSHTDLWDFQANVEGSQKIVDLLRPQLQKANPELLAKVDANFKKVDTILAKY
                     RTKDGFETYDKLTDADRNALKGPITALAEDLAQLRGVLGLD"
     gene            complement(21193..22023)
                     /locus_tag="PN977_003101"
     CDS             complement(21193..22023)
                     /locus_tag="PN977_003101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000497947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FTR1 family protein"
                     /protein_id="HDI6043112.1"
                     /translation="MFVPFLIMLREGLEAALIVSLIASYLKRTQRGRWIGVMWIGVLL
                     AAALCLGLGIFINETTGEFPQKEQELFEGIVAVIAVVILTWMVFWMRKVSRNVKVQLE
                     QAVDSALQRGNHHGWALVMMVFFAVAREGLESVFFLLAAFQQDVGIWPPLGAMLGLAT
                     AVVLGFLLYWGGIRLNLGAFFKWTSLFILFVAAGLAAGAIRAFHEAGLWNHFQEIAFD
                     MSAVLSTHSLFGTLMEGIFGYQEAPSVSEVAVWFIYLIPALVAFVLPPRAGATASRSV
                     "
     gene            complement(22243..>23241)
                     /locus_tag="PN977_003102"
     CDS             complement(22243..>23241)
                     /locus_tag="PN977_003102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309288.1"
                     /note="Members of the Solute:Sodium Symporter (SSS), TC
                     2.A.21 as described in tcdb.org, catalyze solute:Na+
                     symport. Known solutes for members of the family include
                     sugars, amino acids, nucleosides, inositols, vitamins,
                     urea or anions, depending on the system; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/solute symporter"
                     /protein_id="HDI6043113.1"
                     /translation="TILYTFIGGFLAVSWTDTVQASLMIFALILTPVIVIISVGGFGD
                     SLEVIKQKSIENVDMLKGLNFVAIISLMGWGLGYFGQPHILARFMAADSHHSIVHARR
                     ISMTWMILCLAGAVAVGFFGIAYFNEHPAVAGAVNQNAERVFIELAQILFNPWIAGIL
                     LSAILAAVMSTLSCQLLVCSSAITEDLYKAFLRKHASQKELVWVGRVMVLVVALVAIA
                     LAANPENRVLGLVSYAWAGFGAAFGPVVLFSVMWSRMTRNGALAGMIIGALTVIVWKQ
                     FGWLGLYEIIPGFIFGSIGIVVFSLLGKAPSAAMQKRFAEADAHYHSAPPSRLQEG"
BASE COUNT         6044 a         6026 c         5316 g         5855 t
ORIGIN      
        1 aacgccggta tttttattgg ctcgttgttt ttgccagatg tggcgtgaac gccttatccg
       61 acccacaaaa tcttgcaaaa tcaatacatt gcaggagcga tgtaggcctg ataagcgtag
      121 cgcatcaggc aactacgttt ttattgcgaa gccgcatccg gtttacgcaa tgccagtgga
      181 ttgagcttta tcccttcgta ataactcacc catgaagaat atctctcagg ggaattccag
      241 acgcggaaat gcagacgggc catcgtcacc ggatcgctta gcagcaccag gcgacgatcg
      301 cgattcagct tctctggcgt ctcgttcagc gcctgttcaa cgtggcggtc acgggcgatt
      361 tccagcacct tactggcgcg gtgacgcgcg gtcgccattg cggttgccag agcgttgaat
      421 gacgggttaa acactgcgtg cataaaacca tcatcaaggg agcgttggcg attcatctca
      481 aggaaccgat cggtatcaac cagcacctgc ggcggtgaat actcttccgg gatcaggaac
      541 agtttccagc gtttggtgcg cagaccaacg gtggcacggc tggaaatcac cgaaacaaac
      601 ggtgacagga tcaacgagaa gacaatcggt gccagccaga acaggaaacg cagatccaac
      661 cacgccatcc caacagccca cactaaccct aacagcagct gtgagccgtg gcgtttgaac
      721 gcttcacccc aggaagtgga gtcatcatca cgctgcggtg aattccacac cacttcccag
      781 ccaaggaacg cgctgacgac gaagaccgta tggaacagca tgcgtaccgg agccagcagc
      841 acggaaaaca gcacttccag cagcaacgat aatgtaacgc gccagaagcc gccgtattct
      901 ttcgttcctt tgcaccagat aagcaaaatg ctcaataact tcggcaggaa caacagcacc
      961 atggtcgaag caaaaagtgc aatcgccagc tcaggacgcc actgcggcca caccgggaat
     1021 aactgccgtg gttgcaggaa gtattgcggt tcggtcaacg catgcactac ctgcaatgca
     1081 gtagagagcg cgaggaacat aaaccacaac ggagcggaga gataagacat cacgcccgtc
     1141 aggaacaccg cacggtgaac cgggtgcata cccttaacca ggaacagacg gaagttcatc
     1201 aggttaccgt ggcaccagcg gcggtcacgt tttagctcat caagcaagtt aggcggcaat
     1261 tcttcataag aacccgggag atcgtaagca atccacaccc cccaacctgc acggcgcatc
     1321 aacgccgctt ccacgaagtc atgtgacagg attgaaccgg caaaggaacc ttcgcccggc
     1381 agcggagcca gtgcgcagtg ctcgataaac ggtttcacgc ggataatcgc gttatgtccc
     1441 cagtagtgcg actcgccaag ttgccagaag tgcaaaccgg ctgtaaacag tggcccatac
     1501 acgcgagtcg cgaactgctg acagcgtgca tacagcgtat ccataccgga cgctttcggc
     1561 gacgactgaa tgatcccggc gttcgggttg gcttccatca aacgcaccag gccgcataaa
     1621 caatcaccgg tcattaccga gtcagcatcc agcaccacca tgtagctgta ctggctgccc
     1681 cagcgacggc agaagtcatc gatattaccg cttttacgct tcacgcggcg acggcggcgg
     1741 cgatagaaaa tctgaccttc gccaccaact tcagcgataa gctccatcca ggctttttgc
     1801 tctgcgacgc agatatccgg gttataactg tcactcagaa tgtagacatc aaagtgtttg
     1861 gcattcccgg tggcttttac tgattcccac gttgcacgca ggccagcaaa aacacggttc
     1921 acgtcttcgt tacagatagg catgatcaac gccgtgcgat gctccgggtt taatggttca
     1981 tcgccaactg ttgacgctga tatactgtat ttatcgcgac caataagcag ttgcaggaac
     2041 cccatcaacg ccgtccagaa tccggcggac acccaacaga acagtaccgc aaagaggatc
     2101 aggataccgg tttgcagcat ataaggcaga agctgcataa aggaaaccca cacatcctga
     2161 ccaaccatat ccataggatt aatcagcgcc cacccctgat aaggaagaat ggtcttcata
     2221 taccaggtcg cgacaacagt ttgcgcgagc gtcaggatta acaaaatgta acggcggata
     2281 gtaccgacgg tacgccactt ttgctcgctt tcctgctctt ctttggtcaa acgagccaga
     2341 tagcgcggcg ttacatcgcg tccacgcagg cgatcccaga aacggcctac cgggttggta
     2401 cgccacgggt cgggaaacat cgaggagcgt tttgcttctg gcatcgcctt aagctgatcg
     2461 cgcccttcgt cgtctttaat taactgtcca tcagcaagtg aatctggcca ggcttgttcc
     2521 agacgcgcct ttaccgagcc ttgcggggag tcatcctccc gcgcccaggt gcggtgttcg
     2581 gcatccagcg cctgatgaac ggcgcggata tcagtcttcg gcaatgccgc tttctcgctt
     2641 gcggcgatgg gcattgcgtc aatgtactca gttgtcttat tcattggcag gtaactggta
     2701 gctccaggtt tcactcaacg tctgatcggc attcaccagc gcagcacgca tttcagtggt
     2761 tttcttggca tctttcactt tcacacgcat caccagacgc cagcctttgg taaccgggtt
     2821 atagcgcacc gtgctttcaa ctatctcacc attatcacca atgctggttt gcgctgtgac
     2881 cggggtatcc tctggcagtt ttttcatctc tgcgccggta aaatcgacca caaaggcgat
     2941 agtaccgtca ggctggcgaa tcaggttcga ctgcttcaca tcccccgttg aacgacgcgt
     3001 ttgttgcacc catgcgttat ctggtgcatg cagtttgtct tcatcacggc tgaaggtgat
     3061 ggtgtattta aagttcatct ctttacccgg ctccggcagc tgatccggcg tccagtaagc
     3121 gacgatgtta tcgttggttt catcgttggt tggaatttcc accagctcaa cgctgccttt
     3181 gccccactcc cctttcggag tcacccatgc gctcggacga agatcgtaac gatcatcgag
     3241 atcttcaaag cgggagaaat cacgaccgcg ctgcaacaga ccgaagcctt gcgggttttc
     3301 catcgagaag ctgctgaccg ctaaatgttt cgggttattc aacggacgcc agatccattc
     3361 gccattacca gcatggatag acaggccgtt agagtcgtgc aactccggac gatagttatt
     3421 tgcaggcgac ggttggttcg gcccaaacag gaacatactg gttaacggtg caacccccag
     3481 tttgccgact ttatcgcgca gatagatttt cgactgcaca tccacaaccg tgtcacgccc
     3541 tggcataacc acgaatttgt aagcacctgt cgcgcgcggc gagtcaagca atgcataaat
     3601 ggttaaacgt ttatcagtcg gttttggacg ctcgatccag aactctttga agcgtggaaa
     3661 ttcttcaccc gatggcaagg cggtatcaat tgccaggccg cgggcagaaa ggccataaac
     3721 ctgacctgca ccaatcacgc ggaaatagct ggccccgagc atgctgacga tttcatcgtt
     3781 tttatctttg ctgttgatcg ggtaaagcac tttaaaaccg gcaaaaccaa ggtcttttac
     3841 cgtgtctttg tcatgctgaa catcgccgaa agtgaaataa tccgggctgt atttgattcg
     3901 tttgactgcg gtggcagtca cttcatttat tttgaccggg gtatcgaagt acataccctg
     3961 atggtagaac tcgagtttga atggggtctt cagattgttc cagtacgctt tgtcatgatt
     4021 aaactggatc tgctgatagt ccgcgtattt catgtcgcgg aaaacggagg gcaagttgct
     4081 tttgggcgcc tcatagcctt tcccggctaa ggattgagct tgctttgcga catcatcaat
     4141 actgaaagcc cagcttgaag atgtatacag ggttaacatt actgcagcac tcaaccaacg
     4201 cattttcatc atttgtagtt tatgtttcat aattagtaag cacttccccc tttgtgtgct
     4261 tatatcgatc cgatctattt taatggataa ccagccattc cgacaacaga atcccggtat
     4321 cgttcagcac gctggctcat ggattaagca cctgattgca accgttttca ctttccgtgc
     4381 ttctcctaaa gacagaagca tcgacttcgt gtagggtttg ccagcaaatt tatttatcaa
     4441 ccgtcttaca gacgcttcca ttctggttag tggtagcgat gatgctatgc ataaaaacct
     4501 tgaagcctgc gcctggcgcg gtcaggtcgg tgtcgctatg gcaacaagcc taaaggcata
     4561 acccgacagg gataagacga aaaatccgag attagttaac catatatgaa cccagtaccc
     4621 gcgcaacgtg aatatttcct cgactccatc cgcgcctggc tgatgttgtt agggatccct
     4681 tttcatattt ctttaatcta ttcgagccat acatggcatg tgaatagcgc cgaaccgtca
     4741 ttatggctga ccctttttaa tgacttcatc cactcgttcc gcatgcaggt atttttcgtt
     4801 atatccgggt acttttccta catgcttttt ttacgctatc ccttgaaaaa atggtggaaa
     4861 gtacgtgtca aacgtgtagg gatcccgatg ttaacagcca tccccctact gacattgccg
     4921 caatttatta tgctgcaata cgtcaaaggg aaagcggaaa gttggcctgg actgtcattg
     4981 tatgacaaat ataatacgtt ggcctgggaa ttaatatcac acctgtggtt tttactggtg
     5041 ttagtagtca tgacgacgct gtgcgtatgg atatttaaac gcatcagaaa taatttagaa
     5101 aattctgata aaacgaataa aaaattctcg atggtaaaac tatcggtgat ttttttgtgc
     5161 ctcggcatcg gttatgcggt aataagaaga acgattttta ttgtgtatcc gcccattctg
     5221 agtaacggca tgttcaattt tattgtcatg caaacgctat tttatttgcc gttctttatc
     5281 ctcggcgcac tggctttcat tttccctcat cttaaagcct tgtttaccac gccgtctcgt
     5341 ggctgtaccc ttgcagcagc attggcattt gtcgcttact tactcaacca gcgctatggc
     5401 agtggcgatg cctggatgta cgaaaccgag tctgtgatca ccatggtcct cggtctgtgg
     5461 atggtgaatg tggtcttctc cttcggccac cgtttgctta acttccagtc agcgcgggtg
     5521 acttactttg ttaatgcatc gctgtttatc tatctggttc accacccgtt aacgctgttt
     5581 ttcggcgcgt acattacacc gcacatcacc tccaactggc ttggttttct ctgtggcctg
     5641 atattcgtag tagggattgc gataattctg tatgaaattc atttacgcat cccgttactg
     5701 aagtttttgt tctctggtaa accggttgtt aagcgtgaga acgataaagc accagcccgt
     5761 taagccatgc ttataataac cattccacgg gcaatatcga cgccagtctg accataaccc
     5821 gcttccagaa actggtggcg ggttcttttt tgagaacaat ctctttcttt gcatgacgat
     5881 caacccagtt gatccgtccc cacctgtcca gacggagctg ccaggccgca tcatactggc
     5941 tctgaataaa gcgtttatca attaactgtg ccagcgtttc gctctctatc acgaagccca
     6001 tttcagtatt gagcaatgtc gaacgcggat cgaaattgaa agagccgata aacaccgttt
     6061 taccatcgat gctaaaggtt ttagcatgca ggctggcacc ggaattaccg gtgatgccgc
     6121 gatcgtgtaa cgtactactt tgttcacgcg tcggcttgag ttcatataat tccacgccgt
     6181 agcggagcaa ttttttgcgc cagcgtgcgt atccggcatg gacgacagca acatcgttag
     6241 cggcaagaga attggttagg atcgcaatct ttaccccttt tctcaccata cgtaagagtt
     6301 gcgccacacc tgcgcgtgtc ggtacaaaat aggaagagat aatatcgatg cgttcactgg
     6361 gtgagcccat gatatcgaac aggcgctgcg gtaacagtga atgccgtttt gccttgccct
     6421 cccctttcgc cggatcatca cttaataaac gtgtcttcgc ccagataagc ggcaacgttc
     6481 catcaaccag atgatttata aacggactgg attccatttt gcgtaaataa cgatgcgtca
     6541 tggcatcgtt atgccaggag gcgggtaact cgatgcgatc cgccatttca ccttccgtga
     6601 catccagcac ctgctgtaag ggtgaaaccg atttgcaata ccagtagcgg gcgaaatcat
     6661 cggcaacgtc ctctaccacc ggtcctattg ccatgacatc taaatccgaa aaaagtggct
     6721 cttcccctgc cccaaaatag gcatcaccaa tatttcgtcc tcccaccagg gtaaccacgc
     6781 catcgacagt gaaactttta ttgtgcatac ggcgattgag acgggaaaag tcggtgatat
     6841 aaccaagcgg acgtagcaag cgaaacgaga aaggattaaa aagccggact tcaatgcgtg
     6901 gatgactgtc aagcaagcgt aaaatgtcgt caagtccggg cgtattgttg tcgtccagca
     6961 acaaacggac gcgaacgcca cgctttgctg cggctaacag ggcggaaaac agtaaccgac
     7021 ccgacatatc gtcctgccag atgtaatact gaacatccag cgtatgctct gccatttcgg
     7081 ccagacgata ccgggcggca aacgcatcca gacttttctc cagaggaaaa aggccacact
     7141 gaccgggatg ttgcgaacac agtggcagca ccgcgctcgc cagccggggc aaatcattca
     7201 tctccttgtt gagtaaggag tctttcgtag aggtgggcgt tttcttcatc ataacagaca
     7261 aagtatacct gttcgggtaa agcgtgacgg gtaataaatt ctgaaacggt ttttactgcg
     7321 atttcagccg ctgccgcacg agggtaacca taaaccccag tactaattgc aggaaaagcc
     7381 actgacgtat agctgtttgc cgccaccagt cgtaggctat tgagataggc atcctgcaaa
     7441 agctggtctt cgttttgttc accaccacgc cagactggcc cgacggtgtg cactacggct
     7501 ttagcgggaa gatcgcctgc aagcgtaata acggcatgcc ccgtagggca atcgccctgc
     7561 tgttgcctga cttttaaaca ggcatccagc agcgccggac ccgctgcgcg atgaatggcc
     7621 ccatcgacgc cgccgcctcc cattaatgac ggattagccg cattcacaat cacatcaacg
     7681 gccagtttgg taatatcacc ctgcacaaca tgaatacgcg ttttcataac gcctccttac
     7741 accttcttgt ttcttaagtg tacagcaggc tttttggtta aatcgcattt ttctgctcag
     7801 caaggtgagt tgttcagaaa cttagttata ttcgatgttc attacagcca gctttttctg
     7861 tttatcaaga tactgcactt ttactgaggc tatttgcgcg tttttgacat tgccgctcat
     7921 taacgctttg ctggaatagg ttctgcccgg aacagagcca ttctgtgggc agttcatttc
     7981 tacgggtgtt gactgggtgc tgacgtcaca tgccggttcc acaatttggc cataaaagtg
     8041 gatcacgccc ccttgcgcgg tgcttcctgc gatggcaata aaaggaaaaa acatactgcc
     8101 gagcattaga gagtctaaaa aagatctgaa catttattct atttcctcaa tgattagtca
     8161 tccttgaggg ttgtgttatc catactttca ggttggctga ttcgcagcag accattctct
     8221 ccagattcat cttatgctcg atatttcaac aaattaagac tttcctgaag agggcggcca
     8281 ttgttgtgat aaatgaagtg actgtccatc agaaacagta acaactattt tcacccgatc
     8341 gtccggggaa atatttaaac tcaacttcgt caaagcaatg ggttgattag caggcaatga
     8401 gagggtcttt tcttgcttcg tctgactttg ccctgaactg ccttcgcgca gggacaatat
     8461 ttgtactctg cacagacaag attgagtaag agtgacttca ggaataatgg tgtacatatc
     8521 cccttgctgg gtcgtattaa aggttatctg actggaaagt gccgcaagga gtaataacgc
     8581 attcatattc ttctcccgaa aaaaaacagg gcttgcgccc tgtttcttta atacagatgt
     8641 gtattagtac tgatgagcgg tcgcgttgtt accaaagcca acctgagtga cgttaacgct
     8701 ggagttagat gcagtctggt caacagcagc accgttaccg ccaccgaact gtttaacagt
     8761 catagtagaa tctttaccat tccactgatc aagagtagcg ctgttgccga aaccacgctg
     8821 agtcagatcg attgagctgt catcagaacc ttggccaaca tctgcgccgt taccgccgcc
     8881 gtgctgggta atggtcagat cagagttacg ggcgtcagct tgcagagcaa gtgcagagtt
     8941 accgccaccg tactggtaaa tgttcagctc tgaatttggg ccgctattat taccgccacc
     9001 accgtggtta ccgccgccgc cgtactgagg aacaacacct gccagagcgc taccagagaa
     9061 tacgattgct gcaattgctg ctacttttaa aagtttcatg taaaaccccc atcggattga
     9121 tttaaaagtc gaatggaaat taacgttgtg tcacgcgaat agccatttgc gactgtctct
     9181 gcactacaat tgccgttttt tgagtaccat actgtgtaat atttgcttta ttaccagaac
     9241 ctttctggat aatcatcgca gtattaccat aagcaccttg cgaaatactg gcatcgttgg
     9301 cattgcccgc ctgatcaata tatgcaaggt tataatctcc tgtctggtca atctttgccc
     9361 ggttgctact accttcttgc gcaacaaccg ccaaaagttt tgagcctccc tgccgtaact
     9421 gagcactatt attagtccca gcttgaccaa ttatggctgc ctgattaaat gaagacttac
     9481 tcaattcatt taccgcgaag ttatattctg aattagctaa atcataacct gctgcggctg
     9541 caatcccagg cgcacccagt attgttaaca tcataaataa caatttgttt ttcatgttgt
     9601 caccctggac ctggtcgtac atttaagaaa ttaaatcatt tcaacttggt tgttaacgca
     9661 acctgtattt tgttaacgct gcgttacgat ggaaagtatg tctgcggaaa tatttttaat
     9721 aactcacccg cgcgttgtat ttttcttttt ttgttcatac caaagtatta aaacccacgt
     9781 aaatacgctg ttatctacgc aaaaaaatat tttgttttct tttaaatctc cgtttttcgc
     9841 aatcaaaaat caccagacag ccattcttct tgcccgtcgc tgattgctgc gatatgtctt
     9901 gcgcacaagc cgtgacaaac ctcgtctttt tgcagtaaaa aattgtccaa ggaggtgtag
     9961 agaaaaaaca agaacgtttt acatgacgaa aggactacac cgaaatattt tttatatgca
    10021 ttattagcaa gttagcacca tttgtatgat tttttaaaat tgtgcaataa aaactaaatg
    10081 tacaactttt ctatcatttc taaacttaat aaaaccttaa gattaacatt tcaatataac
    10141 gagttacatt tagttacatg tttaacactt gatttaagat ttgtaatggc tagattgaaa
    10201 tcagatgtaa tccattagtt ttatatttta cccatttagg gctgatttat tactacacac
    10261 agcagtgcaa catctgtcag tacttctggt gcttctattt tagaggcagc tgtcaggtgt
    10321 gcgatcaata aaaaaagcgg ggtttcatca tgtttaatga agtccatagt attcatggtc
    10381 atacattatt gttgatcact aaaccttctt tgcaggcgac agctctcttg cagcacctta
    10441 aacaatcgct ggcaattaca ggaaaattac ataatattca acgttctctg gacgatatct
    10501 cttcaggctc tattattctt ctggatatga tggaagcgga taaaaagctt atccattatt
    10561 ggcaggatac tttgagcagg aaaaacaaca atatcaaaat attgttgcta aatacgcctg
    10621 aagattaccc gtaccgcgac attgaaaact ggcctcatat caacggcgtt ttttatgcca
    10681 tggaggatca agaacgtgtt gtcaatgggt tgcaaggcgt cctgcgcggc gaatgctact
    10741 ttacgcaaaa gcttgccagc tacctgatta cgcattcggg taactatcgt tataacagca
    10801 cggaatcagc cctccttact catcgggaaa aagagatcct gaataagctg cgtatcggcg
    10861 cgtctaataa cgagatcgct cgttcgttgt tcatcagcga aaatacggtt aaaacgcatc
    10921 tttataacct tttcaagaag atagctgtaa aaaaccggac acaagcggtt tcctgggcaa
    10981 acgataacct caggcgataa agccatgaaa cgttatttac gctggattgt ggcggcagaa
    11041 tttctgttcg ccgcagggaa tcttcacgcc gttgaggtag aagtcccggg attgttaact
    11101 gaccatactg tttcatctat tggccatgat ttttaccgag cctttagtga taaatgggaa
    11161 agtgactata cgggtaactt aacgattaat gaaaggccca gtgcacgatg gggaagctgg
    11221 atcactataa cggtcaatca ggacgttatt ttccagactt ttttattccc gttgaaaaga
    11281 gacttcgaaa aaactgtcgt ctttgcactg attcaaactg aagaagcact aaatcgtcgc
    11341 cagataaatc aggcgttatt aagtacgggc gatttggcgc atgatgaatt ctaaataaaa
    11401 aattgttcgg aggctgcaat gcgtgtcaaa catgcagtag ttctactcat gcttatttcg
    11461 ccattaagtt gggctggaac aatgactttt cagttccgta acccaaactt tggtggtaac
    11521 ccaaataatg gcgctttttt attaaatagc gcccaggccc aaaactcgta taaagatccg
    11581 agctataacg atgactttgg tattgaaaca ccctcagcgt tagataactt tactcaggcc
    11641 atccagtcac aaattttagg tgggctactg tcgaatatta ataccggtaa accgggccgc
    11701 atggtgacca acgattatat tgttgatatt gctaaccgcg atggtcaatt gcagttgaac
    11761 gtgacagatc gtaaaaccgg acaaacctcg accatccagg tttcgggttt acaaaataac
    11821 tcaaccgatt tttaagcccc agcttcataa ggaaaataat catgcagcgc ttatttcttt
    11881 tggttgccgt catgttactg agcggatgct taaccgcccc gcctaaagaa gcagccagac
    11941 cgacattaat gcctcgtgct cagagctaca aagatttgac ccatctgcca gcgccgacgg
    12001 gtaagatctt tgtttcggta tacaacattc aggacgaaac cgggcaattt aaaccttacc
    12061 cggcaagtaa cttctccact gctgttccgc aaagcgccac ggcaatgctg gtcacggcat
    12121 tgaaagattc tcgctggttt ataccgctgg agcgccaggg cttacaaaac ctgcttaacg
    12181 agcgcaagat tattcgtgcg gcacaagaaa acggtacggt tgccattaat aaccgaatcc
    12241 cgctgcagtc tttaacggcg gcaaatatca tggttgaagg ttcgattatc ggttacgaaa
    12301 gcaacgttaa atcgggcggt gttggggcaa gatattttgg gatcggtgcc gacacgcaat
    12361 accagcttga tcagattgcc gtgaacctgc gtgtcgtcaa tgtgagtacc ggtgagattc
    12421 tctcctcggt gaacaccagt aagacgatac tttcctatga agttcaggcc ggggttttcc
    12481 gctttattga ctaccagcgc ttgctggaag gggaagtggg ttacacctcg aacgaacccg
    12541 ttatgctgtg cctgatgtcg gctatcgaaa caggggtcat tttcctgatt aatgatggta
    12601 ttgaccgtgg tctgtgggat ttgcagaaca aagcagaacg gcagaacgac attctggtga
    12661 aataccgcca tatgtcggtt ccaccggaat cctgacagat aaaaaagccg gaattatcat
    12721 ccggcatttt acggtttaag cgaaagcgtg agtgcccctc acgctttatt tttgatttcc
    12781 tgctcacggt gcgccgtctt tgcactacgc gccgccagcc acaggccgct gtttttcatt
    12841 gcgtaaccaa atatcagccc aagcgccagc gaaggtaaca ccagtttcca gtcaccctgc
    12901 ccggcaaacg tcgcgcatgc gcctataaac gtccccggaa caaatgaaag cagcagctgt
    12961 ttcgcctgaa tacacatcag aaacgcgaca ataccggtta tgacataacc gagaatttcc
    13021 agatgtggtg ccagtgcact accgtaaata atgaccatcg cccacaccac gccactaagc
    13081 agggttgcag cggagatcgc cagccctttc agcccacctt gcgggcaggc aaagtaggcc
    13141 gtacagccca ggaagcccgc ccagcttagt aagcccaggg aaacagccac ccatccccag
    13201 ataccggaga gaatacctgt tgtgattgca atagagagaa gtatgttcat gccgcgcatg
    13261 atagcagaaa acgcggccat gaatgagatc gagcacacat tttatgcaac aacaaattat
    13321 ctgttgcagt tatgagtgat gcagatcaca tttactcttc aggatcttct tccagctctt
    13381 cccacattgc actaatagca tcgcgggtta gcggtgccat ggtgcgccaa aaaggcgtag
    13441 ttgcatgggc ctccacttta ccaaggaacg caccacacca gggtaacaga tactcactga
    13501 acagtgtttc cagtgcttcg ctctcatctt ccgttgactg atcttccagc caggaagccg
    13561 cgagcagcaa tgtgccgatg tgatcggctg gcgtatccgc taatggcatc ccccgttcgg
    13621 aaagaaaagc gcgcacttcc gcttccgtcg cgccctcaac ccatgcgcta cgatatggcg
    13681 gcacagcaca ttcatcgccg ataaacaacg cattgtaatc ggcagagact tgcgtcatat
    13741 cacagctttt ctgcaaacgt gtcagcaact catcctgctc cagtggccag ttcgcagcca
    13801 gtttcccctc acgaatcagg gtaaacagcg gcaccagtaa aggatcttgt ggttggcggt
    13861 aatagagcga acccagcaca cgacagagga tagaaaactc gttcattaat atatttccat
    13921 tactagtaat taaaggtctg caaattccgc aatcggtgcc ataccgcgag attcaaggaa
    13981 gttcagtaag cggcgcggag aaacattcaa aatgcgctct ggcggaaaat ctaccgcgtc
    14041 gaggatttta agacactctt caaattcacc catggtaaac gcggtgtgag aatccgagcc
    14101 taatgccacc caacctccag cgtcacgtac cgctgcggct accgcacgac aattgtcttc
    14161 acttccctta cgtgagtgta aaaaagagga attattgatt tccagcgcca cctgatgttt
    14221 cgcggctgct tcagcaacgg ctttaacatc tatttcatat ttcggatttc cgggatggct
    14281 tattatatgc acattgccgc tggcaatagt ggcgatcatc gcttgtgtat tggtcgcttt
    14341 atcatgcggc gcaaaaaccg gctcatgaaa accggcaata atcagatcca gcgaatcaaa
    14401 cattttgccg ctgcaatcaa tttcaccatc aacattttta atgttagctt cgatgccgcg
    14461 caggatccct actccatcaa ccactcgcgg ccagatacgc atgttaatga agtgccagtg
    14521 atgcggcgca tcttccatat ccgggccatg atcggtgatt gcaaaaagtt taatgccctt
    14581 ttgtttggcc tgggcaatgt aatcactcaa tgtgctatat gcatgcgtgc tggcaacggt
    14641 atgcatatga aggtcgacgg gatacataac tctctcctgt attcaattcc cgccaaggat
    14701 agcaggaaac ctgatgcttt attagtagcc gcgtgcgcgg tcgacttgcc cgcagaccct
    14761 ctcccctttt tcgagctggg caatggtgcg cgaaatgtaa tccacagctt cagcgggacg
    14821 ggtaatcgcg gcgacatgtg gtgttatcgt cacgcgtgga tgttgccaga gcggactttc
    14881 cggcggtaag ggttcacgat taaaaacatc cagcattgcg cctttaactt tgccgctatc
    14941 cagcgccgcg agcaggtcat cttccacgac atgaacacca cgcgccaggt tgaggagata
    15001 cgcgccatcc ggtaattttt cgagtaattg ttgattaata atgccgacgg tttcaggtgt
    15061 attcggtaac aaattaatca atacccgaca ttggctcaga aatgcagaca gttcttcccg
    15121 tccggcaaag ctttgcacgc caggccacga tttacgggtt cgactccagc aacgcagcgg
    15181 aaagcgccag gtttgcagac tctgagcaac tttactgccc agtacgcctg cgcccaaaat
    15241 gccgatggta aaatcttccc gatgatattc aggaagcggt tgccaatgcg aactattttg
    15301 ctggatgcga taatcgtcaa aacgtcgaaa ccaatgcagc acctgactga cagcatattc
    15361 ctgcatttgc tcgcccatac cggtatcttc caggcgaaaa agtggaacag aaggtttcag
    15421 catttcaggg tgtgcctgta gcttgctcaa aatagaatca acacctgccc cgagtgcgaa
    15481 caccgcttta agatcgcgcc ctgccagcat ttcaactgga ggatgccaga ctaaagcata
    15541 atcagcagag acattatctc cgcttttcca tgctctgact cttgcctgag gaatagcttt
    15601 gcgcagtgcc tcaatccacc attgggtatc aaacgttggg tgataaaaga tgatatccat
    15661 actgactccc gaaaagcgtt gcgcgaaatt tattcgcact tatcgttatg atctacaaaa
    15721 gccaccagca taacaaatcc gtggtcggtg gcaaaaaaag caaatttcgc ttattaaaac
    15781 tgcatattga ttgaagtttg aataaacgcg cgatttttta aaaaagtttg ttgacctcag
    15841 gtcatgattt ccctaaatta gcgcccgttc cagcaagaca ggaacaacaa tttggtgagg
    15901 tgtccgagtg gctgaaggag cacgcctgga aagtgtgtat acggcaacgt atcgggggtt
    15961 cgaatccccc cctcaccgcc atctttaaag aagagctcgt acgaaagtac gggctttttt
    16021 ttcgtatatt gcacacaccg gggggatgag aagccccgat cggggtactt agttaatgca
    16081 acggttcacc ctttgtcgcc gcggctaatg ggcgcatttt gtcacgggta tcccagaccg
    16141 gagggatttc atccaattta gtgacagaag ctcgtacaaa aggcacccgt gcaatagtca
    16201 taatcgtatt caaccaacgg tcacgaataa tgcgatctgc cctttcatga ctcggatcat
    16261 ccagctcaat aacacagact attttattgt tcttctttga aaccagaaca taatccattg
    16321 ccattccatt ataaattgca aagaaacgct ttctttcttc ttcgctaaac tcgggttgtt
    16381 ctggaaagtt gatcaagcgc cccagagaga cctggcaatg gatatgacaa taatgtccaa
    16441 accatttctc cagattatca aaatagattt tttcacaacg cgtaattaac cactcttttc
    16501 cttccaataa aataccttcg gcaaccattc cctggaacaa ctcatctcgt tcttgccaac
    16561 gagagtcacg ataggttttc tgcggtgtat agctccttgt cgacgcctta acagcagggg
    16621 aagttgcaga ggaaggaact ggttgcgatt tagtgatgat taccgatgag gcaacttttc
    16681 ggagtgtcaa ttttctggca acagcaaaac cggcgacaaa tgcaattgtc aaacaagtta
    16741 caaaaaccat aaagctcata attaatcttc acctatccat gttgattata tttcattact
    16801 aacgctactc gctgtagcga gttacatgcc aacaattcaa cgatctatat atagttagta
    16861 tcatgttttg attattacaa ccatacctat tttgccattg cggcttgccg tccacaaaaa
    16921 ataacaagac acaatattag ccaaaaattt aattaagatt aaccttatat ttttaatatt
    16981 atccctaaaa aataaaatca ggagaatata tttggtgtta atcagtcagt caaattttgg
    17041 ctcattgttg attaaacctc atacgcaaaa ttggcaccag acctcctgta caggttgaat
    17101 ggctggaaaa agataggcaa aaaactctcc gcgctggagg cattacattg gtcgcgtgct
    17161 atgacgaata atgcctggta aaagcatcta aaccagaatt aatttcagct tatcaacacg
    17221 ctcaaagtcg ccccccagaa cgttaacctg aagtgaaagt ctttgctgtt aatgaaataa
    17281 aacaaagccc ggttcgcccg ggctctgcgc cgataataca cttaactgta tgcatgtagc
    17341 gtacgttggc aaagtgctga acgtacgcaa tcctctttac cgaatcggac gatcccgacc
    17401 atttcatctt cttcgaaacg ttccagcgcg tcacttaatc cggagcgcac gccgcgaggc
    17461 aaatcgcatt gcgtgatatc accgttgacg ataaccgtca cgttctcccc gaggcgggtt
    17521 aaaaacattt tcatttgcgc ggcagtcaca ttctgcgcct cgtcaagaat gacgactgca
    17581 ttttcaaagg tacgtccacg catataggcg aacggcgcaa tttccacctt ccctatttct
    17641 ggtcgcaggc agtactgcat aaaggaagcc cctaagcgcc gaaccagcac atcgtagacc
    17701 gggcgaaaat agggagcaaa cttttctgcg atatcaccag gtaagaagcc aagatcttca
    17761 tcggcttgca gaactggacg ggtgacgata atcctgtcga catccttatg tatcagggcc
    17821 tctgccgctt ttgctgcgct gatccaggtt tttccgcacc cggcttcgcc cgtggcgaat
    17881 atcagctgct tactctcaat agccttcaga tagtgcaatt gcgcttcatt tcgcgcgagg
    17941 atgggcgaag tatcgcgact gtcgcgggcc ataccaatgg cttctacgcc gctcatctgc
    18001 acaagcgagg tgaccgattc ttcttcacgc tgcttatggc tgcgcgaatc ccgtctcagc
    18061 acacgttttg cctcgcgacg agctttgatc actgcttttt gtcttcccat ggagagcacc
    18121 ttgagttgtt tgtattcatc acacgcgccg ttggcagcgc gattatgcgc acgaacatca
    18181 gagggttggc ttccttgtaa gccatagttt gcttttggat aaaatgccga aaaacggcta
    18241 cgcacaccgt ttgcggcgtc ggtaacacat gaaaagaaag gatgaggttg aaaatgcaaa
    18301 gtgacgagat gactaccgga ggagaaaact ccgcgagtgg tggcgcgttg attatctaaa
    18361 acatgtccag tacaggacgt taccatccgc gatctccata gtgactgact atcactgccg
    18421 ggaacttccg ctgctactta ataagtacaa cagatctcgc attcattgca acaatatatt
    18481 cacttatatt taactataaa acaccatttc agtgacatta gtttctactg gaaagatgac
    18541 agagtgatga cagtgatgaa aaaagctgtg tgctttcagc aggattcgca tcaggcatca
    18601 acgcacgaac gccggatgcg gcgtgaccgc cttattcagc ctaccgaacc cgcaccgatt
    18661 gtaggcatga taagacgcgc aagcgtcgca tcaggcatct gcgcacgaat gccttatacg
    18721 cctaaaaaca ttaaacccgc aataatgcgc ttccgagata atcgttcgcg tccttcacac
    18781 ccggcagcgc aaagaaatag ccgccaccga taggtttgac atattcctcc agcgcctcgc
    18841 cattcaacct tttttgtact gtcaggaagc ccttttccag atcgtgttgg tagcagacaa
    18901 acagtagccc catatcgagt tgcccggagt tggtgacgcc cagtgaataa ctgtagccac
    18961 gacgcagcat cagacttgac tcactctctg gcgtgcgggg attcgccagc cgaatatggc
    19021 tgtccagcgc gatcaccttc ccttccggat cgctggcgta atcgggaaca tcatgctcgt
    19081 gctgcatgcc cagcggcgca ccagtttgtt tgtcgcgacc aaaaatcgtc tgctgttctt
    19141 tcagtggcgt tctgtcccaa aactccaccc gaaactgaat caaccgcacc gcctgatagc
    19201 tgccaccgat tgtccacgca ggctcctgct gatctgccgt cacccacacc actttttgca
    19261 tcaacttcgc attctggcta tcgggattgg ctgtgccgtc tttaaaaccc agcaaattta
    19321 tcggcgtctc tttgccttta ctacgcgctg cgtgatcgga aataaaccct tcccgcttcc
    19381 agcgcacgct gagcaaatcc ggcgtgtgtt tgatgatatc gcgcagcgca tggatcaccg
    19441 tgtcctgggt gttggcgcaa atttgtagca acacatcacc atgacataac gccgcatcca
    19501 gcgagtcgtt ggggaaacgc gtcattttct gcaacttttt cggcatctgc ggtgtaaggc
    19561 caaaccgctc atcaaacaat gaatggccca ccgataacgt tatggtgaga ttatcggacg
    19621 caatgtagcc gccaagaatg ccggaatcga gtggtggcag gcgcggattt ggcgtttctg
    19681 gtgccgctcc gccctgagtc agaaaagcaa aacgctgagt caacaagcga aacaaccgtt
    19741 caagatcggt tttatcgctg gcaagcacat caaacgccac cagcatcatt gccgcctgct
    19801 gcggcgtcag aatacccgcc tgatgttcgc cataaaacgg ctgcgcttca ctgcgcgcat
    19861 ccggtgaaag cgtgcccggt gcactttgcg ttttttgtgc atgagcgacc ggacaacttc
    19921 ccgccagcgc caacgcacca atccctttca gtaaacgtcg gcgtgacggt tcattcacgc
    19981 cgttttcatc ttcatactgc ataacgctta gtccagcccc agcacaccgc gaagttgcgc
    20041 cagatcttcc gccagcgcag taatcggccc tttcagcgca ttacggtcag catcggtcaa
    20101 tttgtcgtag gtttcaaaac cgtctttagt acggtatttc gccagaatgg tatcgacctt
    20161 tttgaaattg gcatcgactt ttgccagcag ttccggattc gctttttgta gttgtggacg
    20221 cagcagatcg acaattttct gcgagccttc aacgttagcc tggaaatccc acagatcggt
    20281 gtggctgtag cgatcttctt caccgctgat tttgctggct gccacttcct caatcagtcc
    20341 ggctgcaccg ccgaccactt ttgaaggtgg gaaagccagt tcactgatgc gtttttgcaa
    20401 atcgaccaca tcggtataaa gctggtcagc gtacttatcc atccctttgg tggtgttgtc
    20461 gccaaacaat gctttttcca gacggtggaa accggtgaat tttggatcgg cggctttttg
    20521 ctcgtaatca tcttcacggg cgtcaatgct gccatccaga tcggagaaca gttcagcaat
    20581 cggttcaatg cgctcatagt gctggcgcgt cggcgcatac agtgctttcg ctttttcgat
    20641 atcgcctgct ttaatcgcgt cggtaaaggc tttggtgtcg gtcaccagct gcgtggtttc
    20701 cgccatgaca tacgctttat atgcagtaat tgcaccacca agacttaaca gcgcatcact
    20761 ttgcgccgca tccgccgttg cctcaccttt gacgatcaac ttccctttcg ggttagtcag
    20821 cagaccgcag gtcatatcgt attcgccagg ctgtaaattc gccgtcattt tctggctaaa
    20881 gccaggggcg atattttccc gctcttccac caccatcacg cctttcagga tctcccactc
    20941 cagcgccttc tggctgtggt tctgaataat gaactgtgtt ttcccggcgt taaccgtaat
    21001 ggtcatcggt tcgcactgct tatccgttac ggtcactttg acttgcggaa tatcagcggc
    21061 gttagccata aaagcagaag agaacagcgc agccacgctc aactgcaatg cgttacggcg
    21121 gaagttaatg gtcatgacga gactatccct ttaaagtatg ttgtaactaa gcaagagttt
    21181 gcgtcgtatt tgctacacgg agcgggacgc agtcgcccct gcgcgtggtg gcagaacaaa
    21241 tgccaccagc gccgggatga gataaataaa ccagacggcg acttcgctga cactcggcgc
    21301 ttcctgatag ccaaaaatcc cttccatcag cgtgccaaac agcgagtgag ttgagagcac
    21361 cgcactcata tcgaaggcga tttcctgaaa gtggttccac aatccggctt catgaaatgc
    21421 gcgaatggca ccagccgcca gccctgcggc gacgaagaga ataaacaggc tggtccattt
    21481 aaaaaatgca ccaagattga ggcgaatacc gccccagtag agcaggaagc cgagcaccac
    21541 ggctgtagca agaccgagca ttgcacccag cggcggccag atcccgacat cttgttgaaa
    21601 cgccgccagc aggaaaaaga ccgactccag cccctccctt gcaacggcaa aaaagaccat
    21661 catcaccagc gcccagccat gatgatttcc acgctgcaat gcgctgtcga ctgcctgctc
    21721 cagttgtact ttgacgttgc gcgacacttt gcgcatccag aaaaccatcc aggtaaggat
    21781 caccacggcg atcaccgcca cgataccttc aaacagttcc tgttcttttt gcggaaattc
    21841 gccggtggtt tcgttaatga agatacccaa gcccaggcac aacgcagcgg caagcaacac
    21901 gccaatccac atcacaccaa tccatcggcc tcgctgggta cgcttaagat agctggcaat
    21961 caaactgaca atcagcgcgg cttcaagtcc ttcgcgcaac ataatgagaa acggaacaaa
    22021 catgccaccc acccttaaac gtttttctga gtcaatctat gcaaagaaaa gtaaatcgca
    22081 gtgatatcga ttatcattac gatgaagaaa aatacaagtg gaatgtagtg ataatgatga
    22141 gaagatgtaa aaatattttg agaaaaaacg cagagggcaa tcactgattg ccctctgcct
    22201 gcttcctgtt atgacgtgaa attttcggta aaacgccttc tcttaccctt cctgcaaccg
    22261 tgacggcgga gccgaatgat agtgcgcatc ggcctcggca aagcgttttt gcatcgccgc
    22321 tgacggcgct ttacccagca aactaaacac tacaatcccg atactgccga agatgaagcc
    22381 cggaataatt tcgtacagtc ccagccagcc gaactgtttc cagacgataa ccgtcagcgc
    22441 accgatgatc atccccgcca gcgcaccgtt gcgcgtcatg cgtgaccaca tcaccgagaa
    22501 cagcaccacc ggaccaaacg ccgcgccaaa gcctgcccac gcgtagctca ctaagcccag
    22561 cacgcggttt tccgggtttg ccgccagcgc aatcgccacc agcgccacca ccagcaccat
    22621 cacacgccct acccacacca gctctttctg gctggcatgt ttacgcagaa acgctttgta
    22681 taaatcttcg gtaatcgcac tggaacatac cagcagctgg cagcttaagg ttgacattac
    22741 cgccgccaga atcgccgaca gcagaatccc ggcaatccac gggttaaaca gaatttgcgc
    22801 cagttcgata aacacgcgtt cagcgttctg atttaccgca cctgctaccg ccgggtgctc
    22861 attaaagtaa gcaataccaa agaagccgac agccaccgcc cctgccaggc agaggatcat
    22921 ccaggtcata ctgatacgac gcgcatggac aatgctgtgg tgagaatccg ccgccataaa
    22981 acgcgccagg atgtgcggct gcccgaagta acccagtccc cagcccatca gtgagataat
    23041 ggcgacaaag ttcagccctt tgagcatatc cacgttttcg atgctctttt gtttgatcac
    23101 ttccagagag tcgccaaacc caccaacgct aatgattacg ataaccggcg tcaggatcag
    23161 ggcaaaaatc atcaggctgg cctgtacagt gtcagtccag ctcaccgcga ggaaaccgcc
    23221 aataaaggtg taaaggatcg t
//
LOCUS       DAMZFT010000078        22629 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.078, whole genome shotgun sequence.
ACCESSION   DAMZFT010000078 DAMZFT010000000
VERSION     DAMZFT010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 22629)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22629)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22629
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.078"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(<1..66)
                     /locus_tag="PN977_003103"
     CDS             complement(<1..66)
                     /locus_tag="PN977_003103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HDI6043114.1"
                     /translation="MKWRKRGYLLAAMLAFASATIQ"
     gene            complement(79..609)
                     /gene="fimF"
                     /locus_tag="PN977_003104"
     CDS             complement(79..609)
                     /gene="fimF"
                     /locus_tag="PN977_003104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria minor subunit FimF"
                     /protein_id="HDI6043115.1"
                     /translation="MRNKPFYLLCAFLWLAVSHALAADSTITIRGYVRDNGCSVAAES
                     TNFTVDLMENAAKQFNNIGATTPVVPFRILLSPCGNAVSAVKVGFTGVADSHNANLLA
                     LENTVSAAAGLGIQLLNEQQNEIPLNAPSSAISWTTLTPGKPNTLNFYARLMATQVPV
                     TAGHINATATFTLEYQ"
     gene            complement(619..3255)
                     /gene="fimD"
                     /locus_tag="PN977_003105"
     CDS             complement(619..3255)
                     /gene="fimD"
                     /locus_tag="PN977_003105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher FimD"
                     /protein_id="HDI6043116.1"
                     /translation="MSYLNLRLYQRNTQCLHIRKHRLAGFFVRLFVACAFAAQAPLSS
                     AELYFNPRFLADDPQAVADLSRFENGQELPPGTYRVDIYLNNGYMATRDVTFNTGDSE
                     QGIVPCLTRAQLASMGLNTASVSGMNLLADDACVPLTSMIHDATAQLDVGQQRLNLTI
                     PQAFMSNRARGYIPPELWDPGINAGLLNYNFSGNSVQNRIGGNSHYAYLNLQSGLNIG
                     AWRLRDNTTWSYNSSDSSSGSKNKWQHINTWLERDIIPLRSRLTLGDGYTQGDIFDGI
                     NFRGAQLASDDNMLPDSQRGFAPVIHGIARGTAQVTIKQNGYDIYNSTVPPGPFTIND
                     IYAAGNSGDLQVTIKEADGSTQIFTVPYSSVPLLQREGHTRYSITAGEYRSGNAQQEK
                     PRFFQSTLLHGLPAGWTIYGGTQLADRYRAFNFGIGKNMGALGALSVDMTQANSTLPD
                     DSQHDGQSVRFLYNKSLNESGTNIQLVGYRYSTSGYFNFADTTYSRMNGYNIETQDGV
                     IQVKPKFTDYYNLAYNKRGKLQLTVTQQLGRSSTLYLSGSHQTYWGTNNVDEQFQAGL
                     NTAFEDINWTLSYSLTKNAWQKGRDQMLALNVNIPFSHWLRSDSKSQWRHASASYSMS
                     HDLNGRMTNLAGVYGTLLEDNNLSYSVQTGYAGGGDGNSGSTGYATLNYRGGYGNANI
                     GYSHSDDIKQLYYGVSGGVLAHANGVTLGQPLNDTVVLVKAPGAKDAKVENQTGVRTD
                     WRGYAVLPYATEYRENRVALDTNTLADNVDLDNSVANVVPTRGAIVRAEFKARVGIKL
                     LMTLTHNNKPLPFGAMVTSESSQSSGIVADNGQVYLSGMPLAGKVQVKWGEEENAHCV
                     ANYQLPPESQQQLLTQLSAECR"
     gene            complement(3322..4047)
                     /gene="fimC"
                     /locus_tag="PN977_003106"
     CDS             complement(3322..4047)
                     /gene="fimC"
                     /locus_tag="PN977_003106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418736.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria chaperone FimC"
                     /protein_id="HDI6043117.1"
                     /translation="MSNKNVNVRKSQEITFCLLAGILMFMAMVVAGRAEAGVALGATR
                     VIYPAGQKQVQLAVTNNDENSTYLIQSWVENADGVKDGRFIVTPPLFAMKGKKENTLR
                     ILDATNNQLPQDRESLFWMNVKAIPSMDKSKLTENTLQLAIISRIKLYYRPAKLALPP
                     DQAAEKLRFRRSANSLTLINPTPYYLTVTELNAGTRVLENALVPPMGESTVKLPSDAG
                     SNITYRTINDYGALTPKMTGVME"
     gene            complement(4084..4623)
                     /locus_tag="PN977_003107"
     CDS             complement(4084..4623)
                     /locus_tag="PN977_003107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418735.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HDI6043118.1"
                     /translation="MKRKRLFLLASLLPMFALAGNKWNTTLPGGNMQFQGVIIAEACR
                     IEAGDKQMTVNMGQISSNRFHAVGEDSAPVPFVIHLRECSTVVSERVGVAFHGVADGK
                     NPDVLSVGEGPGIATNIGVALFDDEGNLVPINRPPANWKRLYSGSTSLHFIAKYRATG
                     RRVTGGIANAQAWFSLTYQ"
     gene            complement(4688..5236)
                     /gene="fimA"
                     /locus_tag="PN977_003108"
     CDS             complement(4688..5236)
                     /gene="fimA"
                     /locus_tag="PN977_003108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HDI6043119.1"
                     /translation="MKIKTLAIVVLSALSLSSAAALADTTTVNGGTVHFKGEVVNAAC
                     AVDAGSVDQTVQLGQVRTASLAQEGATSSAVGFNIQLNDCDTTVADKAAIAFLGTAID
                     GTHPNVLALQSSAAGSATKVGVQILDRTGAALALDGATFSAQTTLNNGTNTIPFQARY
                     FATGAATPGAANADATFKVQYQ"
     gene            complement(5717..6313)
                     /gene="fimE"
                     /locus_tag="PN977_003109"
     CDS             complement(5717..6313)
                     /gene="fimE"
                     /locus_tag="PN977_003109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418733.1"
                     /note="upgraded from regulatory protein to switch DNA
                     invertase; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria switch DNA invertase FimE"
                     /protein_id="HDI6043120.1"
                     /translation="MSKRRYLTGKEVQAMMQAVCYGATGARDYCLILLAYRHGMRISE
                     LLDLHYQDLDLNEGRINIRRLKNGFSTVHPLRFDEREAVERWTQERANWKGADRTDAI
                     FISRRGSRLSRQQAYRIIRDAGIEAGTVTQTHPHMLRHACGYELAERGADTRLIQDYL
                     GHRNIRHTVRYTASNAARFAGLWERNNLINEKLKREEV"
     gene            complement(6791..7393)
                     /gene="fimB"
                     /locus_tag="PN977_003110"
     CDS             complement(6791..7393)
                     /gene="fimB"
                     /locus_tag="PN977_003110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria switch DNA invertase FimB"
                     /protein_id="HDI6043121.1"
                     /translation="MKNKADNKKRNFLTHSEIESLLKAANTGPHAARNYCLTLLCFIH
                     GFRASEICRLRISDIDLKAKCIYIHRLKKGFSTTHPLLNKEIQALKNWLSIRTSYPHA
                     ESEWVFLSRKGNPLSRQQFYHIISTSGGNAGLSLEIHPHMLRHSCGFALANMGIDTRL
                     IQDYLGHRNIRHTVWYTASNAGRFYGIWDRARGRQRHAVL"
     gene            8848..9564
                     /gene="nanC"
                     /locus_tag="PN977_003111"
     CDS             8848..9564
                     /gene="nanC"
                     /locus_tag="PN977_003111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminic acid outer membrane channel
                     NanC"
                     /protein_id="HDI6043122.1"
                     /translation="MKKAKILSGVLLLCFSSPLISQAATLDVRGGYRSGSHAYETRLK
                     VSEGWQNGWWASMESNTWNTIHDNKKENAALNDVQVEVNYAIKLDDQWTVRPGMLTHF
                     SSNGTRYGPYVKLSWDATKDLNFGIRYRYDWKAYRQQDLSGDMSRDNVHRWDGYVTYH
                     INSDFTFAWQTTLYSKQNDYRYANHKKWATENAFVLQYHMTPDITPYIEYDYLDRQGV
                     YNGRDNLSENSYRIGVSFKL"
     gene            9584..10690
                     /gene="nanM"
                     /locus_tag="PN977_003112"
     CDS             9584..10690
                     /gene="nanM"
                     /locus_tag="PN977_003112"
                     /EC_number="5.1.3.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418730.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate epimerase"
                     /protein_id="HDI6043123.1"
                     /translation="MNKTIMALAIMMASFAANASVLPETPVPFKSGTGAIDNDTVYIG
                     LGSAGTAWYKLDTQAKDKKWTALAAFPGGPRDQATSAFIDGNLYVFGGIGKNSEGLTQ
                     VFNDVHKYNPKTNSWVKLMSHAPMGMAGHVTFVHNGKAYVTGGVNQNIFNGYFEDLNE
                     AGKDSTAIDKINAHYFDKKAEDYFFNKFLLSFDPSTQQWSYAGESPWYGTAGAAVVNK
                     GDKTWLINGEAKPGLRTDAVFELDFTGNNLKWNKLDPVSSPDGVAGGFAGISNDSLIF
                     AGGAGFKGSRENYQNGKNYAHEGLKKSYSTDIHLWHNGKWDKSGELSQGRAYGVSLPW
                     NNSLLIIGGETAGGKAVTDSVLISVKDNKVTVQN"
     gene            10755..11735
                     /locus_tag="PN977_003113"
     CDS             10755..11735
                     /locus_tag="PN977_003113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709926.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HDI6043124.1"
                     /translation="MNAIISPDYYYVLTVAGQSNAMAYGEGLPLPDREDAPHSRIKQL
                     ARFAHTHPGGPSCHFNDIIPLTHCPHDVQDMQGYHHPLATNHQTQYGTVGQALHIARK
                     LLPFIPDNAGVLIVPCCRGGSAFTAGSEGTYSERHGASHDACRWGSDTPLYRDLVSRT
                     RAALAKNPQNKFLGVCWMQGEFDLTTSDYASHPQHFNHMVEAFRRDLKQYHSQLNNIT
                     DAPWFCGDTTWYWKEKFPHAYEAIYGNYQNNVLANIIFVDFQQQGERGLTNAPVEDPD
                     DLSTGYYGSAYRSPESWTTALRSSHFSAAARRGIISDKFVEAILQFWRER"
     gene            11858..12539
                     /locus_tag="PN977_003114"
                     /pseudo
     CDS             11858..12539
                     /locus_tag="PN977_003114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904641.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            12709..13359
                     /locus_tag="PN977_003115"
     CDS             12709..13359
                     /locus_tag="PN977_003115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001586621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6043125.1"
                     /translation="MNIRQSLHDRIYAEYPQQAKDILLYFPTYEIMPSTIKMVMINEV
                     VAITPQEDFYTPESGYFTTVARQLALAGLSASSGEVLLNHGIYLTSALKLPKTQITAT
                     PAEIILWSPIVEWELELFANIKVIFVGGDIARKCLNIIAKKQTRKHAIPSTPTYKLMN
                     SELVWKGISILPGYILTGKNLLIEKSKIEMNVEQLRKGIAIYRNSNSNHTNNSVHE"
     gene            complement(13873..15987)
                     /locus_tag="PN977_003116"
     CDS             complement(13873..15987)
                     /locus_tag="PN977_003116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HDI6043126.1"
                     /translation="MTVQTPKVALSDGFLGAFARIPKAQQKKVQEFISKFRQDPTSNG
                     LNYEKIHDARSKNVHSVRIDQTYRGIVLKPEQGALYMLMWVDKHDEAYDWARRHDCSI
                     HPVTGAIQVIDISYIKPAAETVVDKPKLFAAYSAEQILALGVPPVFIDQVMALTDEAG
                     LNQLESIMPAEAWEPLHWLAEGLDYQEVLEEFNDHRDEPVDTNDFMEAIERSKRRFYV
                     VENEQELLQMLNAPLEKWRVFLHPSQRKLVESPANGPVRVLGGAGTGKTVVAMHRARW
                     LSQRLVDKPGKKVLFTTFTRNLAADIRANLQRLCTREEMARIDVVNIDAWMSDQLKRH
                     GYDFRVVYDSDEGRRKCWNYALQQAPAELGLPDNFYAEEWQRVVQPNAVYTREEYLKV
                     SRVGRGTALSRIQRAKIWPVFEEFRAQMARAKLREMGDAMHEAIVLFKEKQVQLPYSS
                     IVVDEAQDIGAPAFTLIRSLVPESPNDLFIVGDGHQRIYRNKVVLGQCGINVRGRRSK
                     KLKINYRTTEETRQFAVGLLTGVKVDNLDGEADTSNDYLSLLHGEKPMITHAADFKEE
                     AATLVKQIQALLANQVRSQDICITARTKHACDRYASALNDAGIETFNLGNDSGDSDAR
                     PGVRVATMHRIKGLEFQYVFLVGINEGVVPEIKALASDDPVEQRDALFNERALLHVAA
                     TRAVKGLFVSSSGIPSPLLVAD"
     gene            complement(15984..22325)
                     /locus_tag="PN977_003117"
     CDS             complement(15984..22325)
                     /locus_tag="PN977_003117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HDI6043127.1"
                     /translation="MLPSVVSRQVADSVAAFLRAAFPLNSPLFNGENNDNVSMLEQFL
                     AQPETLLKGPYLSAQLPFRKSDLPLNFFPNLTLPFPPHAHQARAFQRLGSDAPQPTLV
                     ATGTGSGKTECFMFPLLNHCAGASQAGVKAIIIYPMNALATDQASRFAKTIASDPQLH
                     GKVTVGLFVGDSEIEPSKKMSAEKVITCKHTLRESPPDILLTNYKMLDYLLMRPGDQP
                     LWRYNQPGSLRYLVVDELHTFDGAQGSDLACLVRRLKHHIGVDNQRFACVGTSATVGD
                     ELGQLLDYAKTIFDQPFTDDAVIREDRYTAAEYLQDYAIAYSQYPGPEVASALDPQSY
                     ATPVAYLNGQIPLWFPDSDLQLPDDLESDDGREKRIALGSLLRRHSVMHVLLHDLQGG
                     ILSERECLENLQVMLAESADHARRVLQSILALIAQARLEVPETEHDRQKRLQAKKVRP
                     VLPFVQLRSQLWLREMRRLVASVAKTPRLVFADDVAVEDREHYLPVIHCRDCHATGWA
                     SLRHGQSVQLETDLDGIYRQFFEKGRSIVLAFPDNNEKAVSGAHQKLCPTCLKLNRQS
                     SVQCGHCGHTELLQVLIPDMLKERKDELEFANECPYCNSKQGISILGSRAASLSAVMI
                     HQLYGSQFNEHKKLITFSDSVQDAAHRASFFTARTWPLMIRGQIASVLGEKETLPLDA
                     FAARVCQQTRERSPDDAHFAANFIAPNMQWLNDYQTLKNEGKLAENSDLPELVSLRLD
                     WEVYSEFTLRARIGRTLEATGYAVAGVDKERFNPAITALHQQLCEELGDEMSGVSVEQ
                     VAHLALGVIWYQRQAGAVMHPAFESYRRDGTTFMMTQKERTSRYMPSIGPKTRKPAYA
                     SFEKINGFHRLIGAKSNPSWYQHWINRTLSNGNNLFISSVAETVLRRLFTALKIAGVV
                     KDFDTKGREAWGLVPSALVVSRDVVQLNCSCCREVVRAPADQGWQWRNAPCLSLRCEG
                     RYQEVDNTATWRWENMDIARVQGAEHTGLLSREDREATEKSFYRGNQPWNINLLSATP
                     TLEMGIDVGDLSTVLLCSVPPAQANYLQRIGRAGRKDGNALNITVAEGNPHDQFFFEE
                     PLEMMQGQVQAPGVFLNATAILERQLAAFCMDNWVKTGVPESAICKNVKQMLDELEFG
                     RKSGFPYNLLRYIEQHHAEIAAQFTAIFPDLAAETRQQLLSYLQGAPGQRSLVQRIEE
                     ALKLLVEDRKSLRSRIDKLKRSIDKLENAPRDQNFDSDMRELTSERQALMALVNQINN
                     KQTLNFLTDEGLLPNYAFPEAGITLRSVLWRRKEGGEAREYQNTTFEYERPASTALAE
                     LAPLNNFYAGGHKVEIEQIDLKVSEPENWRICSHCNYSENIDQTGDQHKYCPKCGTPG
                     WADAGQKTTLLKLRQVYARASARDSQISDESDSREPAFFQRQLLVSFEKEDVSAAYAI
                     EEGEVPFGFEFLSKVTLRDINFGKMADDANELMIAGEAKKRTGFKVCLGCGMVQRPRD
                     HEPRHDLSCKYRTEPEKAKFEDYLYLYRQLESEALRILLPVSSYSNDRVVEASLGAAI
                     QLGLKHYFKGNVDHLKGVVYREPENEGESWRQYLVIYDTVPGGTGSLKELMRTPDNLL
                     KLLELAYQAIVECSCNHDTHKDGCYRCVYAYRDRGRMKYVSRDQARLLLAKILQARDS
                     IRVIDSIKNISLEAMMGSELEKRFISCLQENKNLIVSRSYAHQGAGWIINTRSEPMMS
                     WHLKAQVDLGAKEAVGIPCRPDYVLYPLMQSDAIKPVAIFLDGFAFHKNSVAEDVQKR
                     QAIRDSDNFWVWTVTWADLKEPGLKHVQDVLGLGHNPDMKQPKFYNLFHDTNFAALEA
                     SFRERNSFALLLDYLADPGGRTQLWQKMAAAHAWVWLDVKKSQDAGTKQKYAYEMQEN
                     APAWRLAELLPDEPFVFGGLLDSCNSSQQFIELASVLPQQAIKPTTSVAQMRSWLRLH
                     ICFDDRYTQDDGYEAGLNGFWRLVNLLQFLPDMTFTSRKAVHLPQESVDVKVTATPEA
                     VPETDESWAEIIEFGLLSAEEIALLQSHSLSAPTLGYELQNDEGEIIAEADLAWPSQK
                     WALIIDNQEFIPLFTTRGWHVAFGPIDENTLQHLSGGDK"
     gene            complement(22325..>22629)
                     /locus_tag="PN977_003118"
     CDS             complement(22325..>22629)
                     /locus_tag="PN977_003118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6043128.1"
                     /translation="KGLVGVGLPRTARKADLKNGFVFNVDSPDWTGGDCTDQAIGWDD
                     VKHLQTGTVSVTFDDYTRSDEGERRTVTWQAPFIKPDREDDYKVAWAFFAQDKESA"
BASE COUNT         5959 a         5628 c         5095 g         5947 t
ORIGIN      
        1 ctgtatcgtc gcacttgcga acgccaacat tgccgccaat aaatacccac gtttgcgcca
       61 tttcatgagc atctccagtt actgatattc aagagtgaag gtagccgtgg cattaatatg
      121 ccccgcagtg acaggcacct gtgtcgccat tagccgggcg taaaaattca acgtatttgg
      181 tttacccggc gtcagggtcg tccacgaaat cgcggacgat ggggcattaa ggggtatctc
      241 attttgctgc tcattcagaa gctgtattcc cagtcccgca gccgctgaca ccgtattttc
      301 aagtgcaagc aggttggcat tgtggctatc tgcaacgccg gtaaacccaa cctttacggc
      361 agaaacagcg ttaccacagg gtgacagcaa aatacgaaat ggaacgacag gagtcgtcgc
      421 gccaatgttg ttaaattgct tcgccgcgtt ttccatcaga tcaacagtaa aattggttga
      481 ttcagcggcc acactacagc cattatctct gacatagccg cggatagtaa tcgtgctatc
      541 cgcagccaaa gcgtgactta ccgccagcca caaaaaagcg caaagaagat aaaaaggttt
      601 gtttctcatc acgccccctt aacgacattc agctgatagc tgggttaata actgctgctg
      661 actctctggt ggcagttgat aattggcgac acagtgagca ttttcctctt ctccccattt
      721 cacctgaact tttcccgcta aaggcattcc gctgaggtaa acctgaccat tatccgcaac
      781 aatgccgcta ctctggctac tctctgatgt caccatcgcc ccaaacggca gcggcttatt
      841 attgtgggtt agcgtcatga gcagttttat cccaacgcgc gctttaaact ctgctcgcac
      901 gatcgcccca cgagtgggaa caacgttagc gaccgagtta tctaaatcga cgttatcagc
      961 cagggtattg gtatccagcg ccactctgtt ttcccgatat tcagtggcat atggcagcac
     1021 ggcataaccc cgccagtcgg tacgcactcc tgtctggttt tcgacttttg catcttttgc
     1081 gccaggcgct ttaacaagca ccaccgtatc gtttaacggc tgccccagcg ttacgccatt
     1141 ggcatgagcc agtaccccac cgctgactcc gtaatagagc tgcttaatat catcgctatg
     1201 gctgtaaccg atattggcat tgccgtaacc accgcgataa ttcagcgtgg cgtagcctgt
     1261 acttccgcta ttaccatcac ctcccccggc gtaaccggtt tgcacgctat agctgaggtt
     1321 gttgtcttcc agcaacgtac cgtatacacc ggccagattg gtcatccgac cgttgagatc
     1381 gtgtgacatg ctgtaactgg cactggcatg tcgccactga gatttactgt cagaacgcag
     1441 ccagtggctg aaaggaatat tgacgttaag cgctaacatc tgatcccgtc ctttttgcca
     1501 ggcgtttttc gtcaggctat agctgagcgt ccagttgata tcttcgaatg cagtatttaa
     1561 accagcctgg aattgctcat cgacattatt cgttccccaa taagtttgat ggctaccact
     1621 caaatacagt gttgatgagc gcccgagttg ctgagtaacg gtgagctgta atttcccgcg
     1681 tttgttataa gcgaggttgt aatagtcggt gaatttcggc ttaacctgaa taactccgtc
     1741 ctgtgtttcg atgttgtagc cattcattcg actgtatgtt gtatcagcga aattaaaata
     1801 tccgctggtc gaataacggt aacccactaa ctgaatattc gtgcctgact cattgagcga
     1861 tttgttatag agaaaacgca ccgattgtcc gtcatgctga ctgtcatcgg gaagtgtgga
     1921 attagcctgc gtcatatcca cagacagagc acccagtgcc cccatgtttt tcccgatacc
     1981 gaaattaaaa gcacgataac gatccgccag ttgcgttcca ccatatattg tccagccagc
     2041 cggaaggccg tggagtaatg tactctggaa aaagcggggt ttttcctgtt gcgcatttcc
     2101 actacggtat tctcctgccg taatggaata acgagtatgc ccttcacgtt gcaaaagcgg
     2161 gactgacgaa tagggtacgg taaaaatctg cgtgctgccg tcagcctctt tgatcgttac
     2221 ctgcaagtca ccactattac ctgcggcata gatatcgttg atggtaaaag gccccggtgg
     2281 caccgtacta ttataaatgt catacccatt ttgtttaata gtgacctgtg cagtaccacg
     2341 agcaataccg tggatcaccg gggcaaatcc tctttggcta tcgggtaaca tattgtcatc
     2401 tgaggccaat tgtgcgccgc gaaagttaat accatcgaaa atatcgccct gagtataacc
     2461 atcacccagc gtcagccggg aacgtaacgg tattatgtct cgctcaagcc aggtattgat
     2521 atgctgccat ttatttttgc tacctgatga actgtcgcta ctgttataac tccaggtggt
     2581 attgtcgcgt aaacgccacg caccaatatt taacccactc tgtaggttta aatatgcata
     2641 atggctgtta cccccaattc gattctgtac actatttccg ctgaaattat aattgagcaa
     2701 tccggcatta ataccgggat cccataactc aggaggaata taaccacgcg cgcgattact
     2761 cataaatgcc tgagggatcg tcaggttgag tcgctgctga cccacatcca gttgcgcagt
     2821 agcgtcatgt atcattgagg ttaatggcac gcaggcatca tccgccagca gattcatacc
     2881 ggagacagaa gccgtattca gccccatact ggcaagttgc gcgcgtgtca ggcagggaac
     2941 aatcccttgt tcactgtcgc ccgtattaaa tgtgacatca cgcgttgcca tataaccatt
     3001 attcaaatag atatcgacac gatacgtccc tggcggtaat tcttgcccat tctcaaaacg
     3061 cgataaatca gccacagcct ggggatcatc cgctaaaaaa cgcgggttaa aatagagttc
     3121 ggcagatgac aaaggtgcct gtgcggcaaa agcacaggca acaaagagcc ggacaaaaaa
     3181 accagccaaa cgatgcttac gaatatgcaa gcattgtgtg tttcgctggt aaagtcttaa
     3241 attcagatat gacattattc ctccggttta aaatcacccg gcagtcaatt ctttttattc
     3301 aggcgaaaaa ttccccctgc gttattccat tacgcccgtc attttggggg taagtgcgcc
     3361 ataatcattt attgttcggt aagtaatatt gcttcctgca tcagaaggca atttaaccgt
     3421 gctttcgccc attggaggca ccaatgcatt ttcaagaacc cgggttccgg cattcaactc
     3481 tgttaccgtc aggtaatagg gtgtcgggtt aatcagcgtc agagaattcg cgctacgacg
     3541 aaatcttaat ttttctgcgg cctgatcggg tggcaacgct aatttagccg ggcgatagta
     3601 cagtttaatg cggctgataa ttgcgagctg tagcgtattc tcagtcaatt ttgatttatc
     3661 cattgacgga atcgctttaa cgttcatcca gaataaactt tcccgatcct gtggcaattg
     3721 gttatttgtt gcatcaagaa tacgtaaggt attctctttt tttcccttca tcgcaaacag
     3781 aggtggcgtc acgataaaac gaccatcctt aacaccatcg gcattttcca cccatgattg
     3841 aattaaatag gtactatttt catcattatt tgtcacggca agttgcactt gtttttgccc
     3901 tgccggataa attacgcgag tcgcacctaa ggccactccc gcttcagcgc gtccggcaac
     3961 caccattgcc atgaacatca ggatacctgc cagcaagcag aatgttattt cctgcgattt
     4021 ccttacattg acgtttttat tactcactgt cctgttcctg ttatcacatt atctgctgaa
     4081 caattactga taggttaaag agaaccaggc ctgggcattg gcgatgccgc cagtaacccg
     4141 acgcccggta gcacgatatt tggcgatgaa atgtagcgaa gtagagcctg agtaaagccg
     4201 tttccagttt gctggaggac gattaatcgg tacgaggttt ccttcatcat caaataacgc
     4261 tacgccaata ttggtggcta tccctggccc ctctcccacg gaaagcacat ccggattttt
     4321 accatccgcg acaccgtgaa acgccacacc cacacgttca ctcaccaccg tgctacattc
     4381 ccgtaaatga ataacaaaag gcaccggtgc gctatcttcc ccaaccgcat gaaaccggtt
     4441 actgctgatt tgccccatat tgaccgtcat ttgtttatcc ccagcttcaa tccggcaagc
     4501 ttctgcaata atgacgccct gaaattgcat atttccgccg ggcaacgtgg tgttccattt
     4561 atttccggcc agagcaaaca ttggcagtaa cgacgctaat agaaataatc tcttcctttt
     4621 cattgttatt tttatcgcac aagggtgggc atccctgccc gtaatgacgt ccctgaacct
     4681 gggtaggtta ttgatactga accttgaagg tcgcatccgc attagcagca cctggggttg
     4741 cggcaccggt tgcaaaataa cgcgcctgga acggaatggt gttggttccg ttattcaggg
     4801 ttgtttgtgc actaaatgtc gcaccgtcca gcgccagcgc agcacccgtt ctgtccagga
     4861 tctgcacacc aacttttgtt gcgctacccg cagctgaact ctgcaaagct aatacgttag
     4921 gatgagttcc atcaattgcg gtaccaagga aggcaatagc agctttgtct gcaacagtag
     4981 tgtcacaatc gttcagttga atgttaaaac cgacagcaga actggttgct ccttcttgtg
     5041 ccagcgatgc ggtacgaacc tgccctaact gaacggtttg atcaacagag cctgcatcaa
     5101 ctgcgcaagc ggcgttaaca acttcccctt taaagtgaac ggtcccacca tttaccgtcg
     5161 tagtatcagc cagagccgct gcggaactga gggacagagc cgacagaaca acgattgcca
     5221 gagttttaat tttcatgctg ctttccttta aaaaaactat ttctaaatcg acatgggcag
     5281 tcgttctgta cgctttgttt tgtcaacgag tttgactgcc aacactgcac agtttccccc
     5341 caaaagatga aacatttggg gccattttga ctcatagagg aaagcatcgc gcacaaactt
     5401 tttcagttta tttgttggct taatattcta ttgttatctt tatttataga tttttatatt
     5461 gcatgaggtg gtttttggag agaagaatga ggaagatgca tcgagccaca gaaacgttag
     5521 ctttacatat agcggaggtg atgtgaaatt aatttacaag agaaataatt tacatatcaa
     5581 acagttagat gctttttgtc gttttttgat atttttatgc ttgagaaaaa atacgtaact
     5641 tatttatgat atggacagtt tggccccaat tgtcttgtat ttatttgttt tttaacttta
     5701 ttatcaataa gttaaatcaa acctcttctc tttttaattt ttcgtttatg agattatttc
     5761 tttcccataa tccggcaaaa cgagcagcat tactggcggt ataacgcaca gtatggcgaa
     5821 tatttcgatg cccgagataa tcctgaatta aacgggtatc tgcaccacgc tctgccagtt
     5881 cataaccgca agcatgcctt aacatatgag gatgagtctg cgttacggtt ccagcttcaa
     5941 taccggcatc gcgaataatg cgataggcct gctggcgaga aagccgactc ccgcggcgag
     6001 aaataaatat agcgtcggtc cggtcagcgc ctttccagtt agcacgttcc tgggtccagc
     6061 gttccacggc ttcacgctca tcaaaacgta acgggtgaac ggtagaaaat ccgttcttca
     6121 gtcggcgaat atttattcga ccttcattaa ggtcaaggtc ctgataatgc agatcaagca
     6181 gttcactaat acgcatccca tgccgatatg ccaacagaat aagacaataa tctctggctc
     6241 ccgttgcccc gtaacaaacc gcctgcatca tggcctgaac ttctttaccg gtaagataac
     6301 gacgtttact cacaatagta gtactcctga ctgagatata tttgaatgaa tacctatagg
     6361 aaacctcaat cggtcaaaat tagcccaata gataaaaaga taaccataca cgaagtgatg
     6421 tggctatcag tcaattacat taaatatcat acaaaaataa aatatcaccg atgtgacaac
     6481 atctattttc attgatttga ttcaatataa caatagattg cagaagatta acttccagtt
     6541 gcttaaaaaa taaagaaaaa agtgtggaaa caggagaatt taagtgatgt gtaaatttat
     6601 gaatgatttt gtttatttat tttgaagaac ataaaccatt gtattttgta tggatatggt
     6661 taacttaatg tttattatga taattaagaa ttacacttac aataacatca attaataaat
     6721 ggttaatcat ttttgatata tcgtaagaat aatgtagttt ttaacaccat ccatggtatc
     6781 tcaactatct ctataaaaca gcgtgacgct gtcgtcctct ggctctatcc cagatgccgt
     6841 aaaaacgccc tgcattgctg gcggtatacc agacagtatg acgaatattg cgatgcccaa
     6901 gataatcctg gataagtcgc gtatctattc ccatattcgc caaagcaaaa ccacacgaat
     6961 ggcgtaacat gtgcggatga atctccagtg acaacccggc attaccaccg gaagtcgaga
     7021 taatatggta aaactgttgc cgagaaagtg gattcccctt acgtgataaa aatacccact
     7081 cgctctcagc atgcggataa gaagtacgga tactcaacca gttttttaaa gcctgaattt
     7141 ctttgttcaa tagcgggtgc gttgttgaaa agcctttttt taatcgatgg atatatatac
     7201 actttgcctt aagatcaata tccgaaatcc tcaatcggca aatttcactc gcccggaaac
     7261 catgaataaa acaaagcaaa gtcagacaat aattacgtgc agcatgaggc ccggtatttg
     7321 ctgctttaag gagtgattcg atttcactat gggtaaggaa gttccttttt ttgttatcag
     7381 ccttattctt catcgttttc ccttataatt acagacgcgc actagctgtg ctgagttagc
     7441 aataatccaa cattttaatc ctgattatgt ttataaaagc gaacgtttgc ttaataacta
     7501 acgtaagtgg accagttctt ctgagcgaac ttaaatggag tagcgatagt tacttataat
     7561 aactttgcca tacaaacgcc atgctcttgc atgctatgta cctttatata tttatcaatc
     7621 tgatacgaat ctaataataa ccctcatcat taatgcaact aatcttatct atatatcatg
     7681 tgatatgttc tgtaacaagt aaaatcaaca taaaaaattc agcaaaattt aatattcgtt
     7741 caactagccg tctcacatat gacattatta gtcaactgca tttgccacaa aatggctatt
     7801 tcatggaacg cagttatttc ttagtggcta ttatcatgct aattcttagg tccctagcga
     7861 ttattcctgc ctggttcatc agcttacgcc atgagattgt tacattcctt tttatcctgg
     7921 agatatgggg gaacgcagga gatgattttt tcatctacat ccgttggtaa atatggcttt
     7981 ggtcactacg acttaaacct tcctcaatca cagctattcg ccgggcgata cggcaaacaa
     8041 actaagtgaa acatcatcct gaaaacacca acatcaacaa gcctctccag atcgacatca
     8101 ggagtgacca gttacaaacc acaaaccaag aaacatattt catgatgaga attatgctca
     8161 gcctgatggc gggtaaaccc ttattatcac caagctcact gcgttagctt gcaaagcttt
     8221 ccttgtttag attctaaata aaatttttat aaaacagata gttaaaagaa aatctttagt
     8281 tttttgaaga ccaccgcaag tgttcgtctg gcttcacatg gcatcttctt cttagaaaaa
     8341 gatcgacata ttttgtgaca cgaattgcaa atctggtttt gttgtatgga ttgcgtgatt
     8401 tttgatctgg tataacaggt ataaaggtgc accacgatag tcaatgagac tgggcatctc
     8461 gtaatctatg gcaaacatca cttcagttct ttctcatcgg gtgatgaaaa cgcacttcag
     8521 tcagaaagga atatgaaaat gagatcaaca gacactctat tttatgactc tgggtaaaat
     8581 gaattgagta agtgatatag cttacgaaca ttcaaatcaa ttaaacatca gaatagattt
     8641 tatactcagg tatttaatct ggctctctgt ttatttaaat aatgtggaaa gagaattttc
     8701 acaggagacc ttatacaaaa aatataaaat acagctactc gttgccaaag acactataag
     8761 cctggcaaaa aatattacac aacataaatg ctaattgttt atgcgggctt tgtattgctt
     8821 tctgtatcct acaaatgagt gaaatttatg aaaaaggcta aaatactttc tggcgtatta
     8881 ttactgtgct tttcgtcacc attaatttct caggctgcga cactggacgt acgtggtgga
     8941 tatcgtagcg gaagccatgc ctatgagact cgactcaaag tcagtgaggg atggcaaaat
     9001 ggatggtggg caagcatgga aagtaatacc tggaatacca ttcatgataa taaaaaggaa
     9061 aatgccgcac tcaatgatgt tcaggttgaa gttaattacg cgattaaact tgatgatcaa
     9121 tggacggtgc gcccgggaat gttaacgcat tttagcagca acggcacacg ctacggaccc
     9181 tacgtaaaac tgtcctggga cgcgacaaaa gatcttaatt ttggcattcg ctatcgttac
     9241 gactggaaag cttaccgaca acaagactta tccggtgata tgtctcgtga taacgttcat
     9301 cgttgggatg gatatgtcac ttaccatatt aatagtgatt tcaccttcgc atggcaaacg
     9361 acgctataca gcaaacagaa cgattatcgc tatgcaaacc acaagaaatg ggcgacggaa
     9421 aatgcatttg ttctacaata ccatatgacg cccgatatta cgccatacat agaatatgac
     9481 taccttgacc gccagggtgt ttacaacggc agagataatt tatcggaaaa cagttatcgc
     9541 attggtgtgt catttaaact gtagtagaca ggagacagtc acaatgaata aaacaataat
     9601 ggcgcttgct atcatgatgg cttcatttgc cgcaaacgcg tctgtattac cggaaactcc
     9661 tgtgccattt aaaagtggta ccggagcaat tgataacgac actgtctaca ttggtttagg
     9721 tagcgcaggt acagcatggt acaagctgga tacacaggcc aaagataaaa aatggacagc
     9781 gttagctgca ttccctggtg gaccgagaga tcaagcaacc tctgcattta ttgatggcaa
     9841 tctgtatgtg tttggcggca ttggcaaaaa cagcgagggc ttgactcagg tatttaatga
     9901 cgtacacaaa tacaacccca aaactaatag ctgggttaaa ttgatgtcgc acgcgccgat
     9961 gggcatggcg ggccatgtga cttttgtaca caacggcaag gcttatgtta ctggcggtgt
    10021 taaccagaat atcttcaatg gctattttga agatctcaac gaggctggaa aagattcaac
    10081 cgctatagat aaaatcaacg cccactattt tgacaaaaaa gcagaagatt atttcttcaa
    10141 taagtttctg ttgtcttttg atccctcaac acagcaatgg agttacgctg gcgaatctcc
    10201 ctggtacgga acggctggtg cggcggttgt gaataaaggt gataaaacct ggcttattaa
    10261 tggcgaagcc aaaccaggat tgcgaacgga tgccgtattt gaacttgatt tcaccggtaa
    10321 taatttaaaa tggaataagc ttgatcccgt ctcatcacca gatggcgtcg ctggcggttt
    10381 tgcggggata agcaatgatt ctcttatatt tgccggaggg gccggattca aaggttcacg
    10441 agaaaattac cagaacggta agaactatgc gcatgaaggc ctgaaaaaat catatagcac
    10501 tgatattcat ctttggcata acgggaaatg ggataaatcg ggtgaattat cgcaaggtcg
    10561 ggcctacgga gtatcattgc cctggaataa tagtttatta attattggcg gtgaaactgc
    10621 aggcggcaaa gcggtgacgg attcagtttt aatttctgtg aaggataata aagtcacagt
    10681 acaaaactaa cgcttcaggg ccccggtaaa ggggccctgc tatcaatttg tcatttgtat
    10741 tacgcgaaaa ttatatgaac gcaataatat cgcccgatta ttactatgtt cttaccgttg
    10801 ctggtcagtc taatgccatg gcgtatggcg aaggactgcc attaccggac agggaagatg
    10861 cgcctcattc cagaattaaa caattagcga gatttgcgca tacgcatccc ggaggcccgt
    10921 catgtcactt taacgacatt attcctctga ctcactgccc acacgatgtt caggatatgc
    10981 agggttatca ccatcctctg gcaacgaatc atcaaacaca gtacggcacc gttggccagg
    11041 cactgcatat tgcacgaaaa ttactgccct ttattcctga taacgcaggg gttctcatcg
    11101 ttccgtgttg ccgtggcgga tcggctttta ccgcgggcag cgaagggaca tattcagaac
    11161 ggcacggagc cagccatgat gcttgtcgtt ggggatcgga tactccgcta taccgggatt
    11221 tagtcagcag aacgcgagcc gcactggcaa aaaatccgca gaacaaattc ctcggcgtat
    11281 gctggatgca aggcgaattt gacttaacga ccagtgacta cgcgtcacac cctcaacact
    11341 ttaatcatat ggttgaagcc tttcgtaggg atctaaaaca ataccattct cagcttaata
    11401 atattactga cgcgccgtgg ttttgcggcg ataccacctg gtactggaaa gaaaaattcc
    11461 ctcatgcgta tgaagctatt tatggcaatt atcaaaataa tgttttagcc aatattattt
    11521 tcgtcgactt ccagcaacaa ggtgaaagag gactgacgaa cgcgcctgtt gaagacccgg
    11581 acgatttaag cacgggatat tacggttcag cgtaccggtc accggagagc tggacgacgg
    11641 cactgcgaag cagtcatttc agcgcggcag cccgtcgggg gattatttct gacaagtttg
    11701 tagaagcaat tttgcagttt tggcgcgaaa ggtgagcgtt cattaatttg atttatttgc
    11761 atccatcaac gcttttaact gctccggcgt gcggcactat aatggttttc acaacaagga
    11821 gccattttct tataccactt tcagagacca tccgcttatg ccaccgccct ctacgcaact
    11881 gtttgcagag caccttccca ccgagtggat acagcacctc ctgacgcttt ctgctcatgc
    11941 gaccatttgt cgtcttaacc tgagcgcgga tggcgaagcg gggattagcc tgctggcccg
    12001 cagcgctgtc acccaggagc gccagcgcgt gggggccccc tggtggaatg gctcttccgc
    12061 cagaccgcac agacatgggg cgcggaacgt tacctgaagg atgactggca cggcctgcaa
    12121 ctttttgcca ttgatggcgc acagttcagg acacctatga acctgagttg cgcgaatatt
    12181 atggctctgc caacacatcc actgagcggc agagcgccta cccggtcatg cgtctggtgg
    12241 cgttaatgaa tctgggaatc acattctgct gaatgccatg acctcggcct accggcggag
    12301 cgatacagcg tgaggcaact aaagcggcgt cagaaatcag ctttaagttc gcattccagt
    12361 ttatcgtgac agaaatgata gtgcagggaa atacggcgtc accaggaacc atcccgaaac
    12421 ggctggagca tctgcggggt gctctggaag cggtgttccc acgactatca aggccgaggg
    12481 ctgttaaggt atcaaaaacc cgttatccgg tgaaacacag tgccgccccg cttaagtgac
    12541 ggcattgtgt ttctgactgg ccttaacttt ccctgaatct ctgacgatcc tcaccattga
    12601 tattttcttc attcctcacc agagaaataa aaaatcacta tactcatcaa aatatgagat
    12661 tacatttatt caggcgatca ccttactatt ttatacggaa aataaatcat gaatatccgt
    12721 caatcattac atgaccgaat ctatgcagaa taccctcaac aggctaaaga tatattactg
    12781 tatttcccca cttatgaaat tatgccatcg accataaaaa tggttatgat taatgaggtg
    12841 gtagctataa caccgcaaga agatttttat acaccagagt ctggttattt tacgactgtc
    12901 gcccgacagt tagcgctggc tggattatcg gcaagtagtg gcgaagtctt actcaaccat
    12961 ggtatctacc tgacctccgc actgaaacta cctaaaacac agatcacagc cacacccgcg
    13021 gagatcattc tctggtcacc tattgtagaa tgggagctcg aactctttgc caatataaaa
    13081 gttattttcg ttggcggcga tatcgcgcga aaatgcctta acatcatcgc gaaaaagcaa
    13141 acacgtaaac atgccatccc ttcaactccg acgtacaaac tgatgaatag cgaattagtc
    13201 tggaaaggaa taagcatact tcctggatat attcttaccg gaaaaaacct gctcattgaa
    13261 aagtcaaaaa tcgaaatgaa tgtcgaacaa ttaagaaaag gaattgcgat ttatcgcaat
    13321 agtaacagta accatacgaa taattcagtt catgaataag agatgaatca ttaataacaa
    13381 gaaatcactg acaaaccacc tgtaaaaggt caatcatttt agttcaattt cctttgtcat
    13441 gattatattt ttatcgtcct gacgatacta ttaacaatta caatatttat accctcccca
    13501 gaacaaacag aaaagaactc atacattcgt ttataattct ggtaatcctg gcatcatacg
    13561 tttaaaattt aaggtgtttg ttttataatc atgattccaa agactattgg tcaccagaaa
    13621 atgccagcct tttcgtgcca gctatatttg tccagtgtgt taccccatcc aaaaatcatc
    13681 cacatcctga tgttacgtta gatatagaga aaacaggttg tactgtgcaa tatgctaacc
    13741 gcataagcga atataatttc taagctgata ttgtctggct gttgggagct aaacaatata
    13801 aatatcgcca gttgccagaa cggttatata gaccaggagc gtttcggctc ctggtctgtt
    13861 tcggacatat tatcaatcag ccactaatag cggactaggg ataccagatg atgaaacaaa
    13921 cagccctttt acggcacggg ttgcagccac atgcagtaac gcgcgctcat taaataatgc
    13981 atcacgctgt tcaacgggat catcggatgc taatgctttt atttccggca caacgccttc
    14041 gttaatcccc accaggaaca catactggaa ctcaagccct ttaatacggt gcatcgttgc
    14101 aactcgcacg cctgggcgtg catcgctgtc accgctatca ttgcctaaat taaatgtctc
    14161 aattccggcg tcattgagtg ctgatgcgta gcggtcgcaa gcatgtttag tacgcgcagt
    14221 aatgcagata tcctgagaac gaacctgatt cgccagcaat gcctgaattt gcttgacgag
    14281 tgttgcagcc tcttctttga aatctgccgc atgcgtaatc atgggctttt ccccgtgcag
    14341 taatgacaaa taatcattac tggtatccgc ttcgccatcg aggttatcaa ccttcacacc
    14401 agttaacagc ccaacagcaa attgacgcgt ctcttctgtg gtccgatagt taatcttcaa
    14461 ttttttactg cgacgaccac ggacgttaat accacactga ccaagaacga ccttattgcg
    14521 ataaatacgc tgatgcccat ccccgacaat aaacagatcg ttgggcgact cgggaaccag
    14581 gctgcgaata agagtaaatg ccggggcacc gatatcttga gcttcatcga cgacaatact
    14641 gctataaggt agttggacct gcttttcttt aaacagaaca atagcctcat gcatggcatc
    14701 gcccatttcg cgcagtttgg cacgcgccat ttgtgctctg aattcttcga aaactggcca
    14761 gattttcgcc cgttgaatac gggataacgc tgtaccacgt ccaacccggc tgactttgag
    14821 gtattcctcc cgggtgtaaa ccgcatttgg ctggacaacg cgttgccact cttcagcata
    14881 gaaattgtca ggtagcccca gttcggcagg tgcctgctgc aaggcatagt tccagcattt
    14941 acgtcgccct tcatcgctgt cgtataccac gcggaaatca taaccgtggc gttttagttg
    15001 gtcgctcatc caggcatcaa tgttaacgac gtcaatacgc gccatctctt ctcgcgtaca
    15061 caaccgctgg agattagcgc gaatatctgc cgccaggtta cgcgtaaagg tcgtgaaaag
    15121 taccttctta ccgggtttat ctaccagacg ctgagataac caccgcgcac ggtgcattgc
    15181 aacaaccgtt ttccctgtac ctgcaccgcc cagtactcgt accggaccat tagcgggcga
    15241 ctccaccagt tttcgctggc tcggatgcag aaatacacgc catttttcta aaggcgcatt
    15301 aagcatctgc aacagctcct gttcattttc aacgacatag aagcgacgtt tacttcgttc
    15361 aatcgcctcc ataaagtcgt tggtatcaac aggctcatca cgatgatcgt taaactcttc
    15421 cagcacttcc tggtaatcca gcccttctgc cagccaatgc aggggttccc aggcttctgc
    15481 tggcattatg ctttcaagtt gattaagccc ggcttcatcg gtaagcgcca tcacctgatc
    15541 aataaagaca ggcggcacac ccagggccag aatctgttcc gcgctgtaag ctgcgaagag
    15601 tttcggttta tcaaccactg tttccgctgc cggtttgata tagctaatat caataacctg
    15661 aattgcgccg gtaaccgggt gaattgaaca atcatgccgc cgcgcccagt cgtatgcttc
    15721 gtcgtgttta tccacccaca tcagcatata cagggcaccc tgttcaggtt tcagaacaat
    15781 gccgcgataa gtttgatcga ttcgcaccga atggacattt ttactgcgcg catcgtggat
    15841 cttttcatag ttaagtccat tactggtcgg gtcctgacgg aatttggaga tgaattcctg
    15901 cacctttttt tgctgtgctt ttgggatccg cgcgaatgcg cccagaaaac catcagaaag
    15961 tgccaccttg ggcgtttgca ccgtcatttg tcacctccgg acagatgttg caatgtattt
    16021 tcgtcaattg gcccgaatgc aacgtgccaa cccctggttg tgaataatgg aataaattct
    16081 tgattatcaa taatcaatgc ccatttttgt gatggccagg cgagatcggc ttccgcaata
    16141 atttcacctt catcattttg caattcgtag ccgagagtcg gcgcagagag agaatgagac
    16201 tgaagcaacg cgatctcttc agcgcttaat aagccaaact caataatctc agcccaactt
    16261 tcatcagtct ctggtactgc ctctggcgtt gccgtaactt taacgtcaac gctttcctgc
    16321 ggtaaatgca cggctttacg gctggtaaag gtcatatccg gcaggaactg taacaggttt
    16381 accagtcgcc agaagccatt gagtcccgct tcataaccgt catcctgcgt atagcgatcg
    16441 tcaaaacaga tatgcaaacg cagccagctg cgcatttgtg cgacactggt cgtcggttta
    16501 atggcttgtt gtggcaacac cgaagccagc tcaataaatt gctgcgatga gttacagcta
    16561 tcgagtaaac caccaaagac aaacggttcg tctggcaaaa gctctgctag ccgccaggct
    16621 ggcgcgtttt cctgcatttc ataggcatac ttttgtttcg ttcccgcgtc ctgtgacttc
    16681 ttcacatcca accacaccca ggcatgtgca gcagccattt tctgccataa ctgcgttctt
    16741 cctcccggat cggcgagata atccagcagc agtgcaaaac tgttgcgttc ccggaacgac
    16801 gcttcaaggg ccgcaaagtt ggtatcgtga aacaggttat aaaacttagg ctgtttcata
    16861 tccgggttat gcccaagccc caggacatcc tgaacatgtt taagcccagg ctctttgaga
    16921 tcggcccacg tgactgtcca cacccagaaa ttatcgctat cccgaatcgc ctggcgtttt
    16981 tgcacatcct ctgcgacgct atttttgtga aacgcaaagc catcaaggaa aatagctacc
    17041 ggtttaatgg catcagactg cataagcgga tatagcacgt aatccgggcg acaagggatc
    17101 cccaccgcct ctttagcgcc taaatccacc tgagctttaa gatgccagct catcatcggt
    17161 tcgctgcggg tgttaataat ccacccagcg ccctgatgcg cgtaactgcg actgacaatc
    17221 aggtttttat tttcttgcag gcagctaata aaccgttttt ccagctcact gcccatcatg
    17281 gcttcgaggg aaatgttttt gatggaatca attacccgta tcgaatctct tgcctgcaaa
    17341 attttcgcca gcaagagacg cgcctgatcg cgggagacat acttcatgcg cccacggtca
    17401 cggtaagcgt aaacgcaacg ataacaacca tctttatgcg tatcatggtt gcaactgcat
    17461 tccactatcg cctgataggc cagttcaagt aatttcagca aattgtcggg agtgcgcatc
    17521 agctctttaa gagaaccagt tccccccggg acggtatcgt aaatcaccag atactgccgc
    17581 caggattcgc cttcattttc cggctcgcgg tacacaacgc ctttcaggtg atcgacattc
    17641 cccttgaagt aatgtttaag acctagctgg atcgccgccc caagagaagc ctccacgaca
    17701 cggtcattac tgtaactgct taccgggagc agaatacgca acgcttctga ctccagctgg
    17761 cgatagagat agagataatc ttcgaacttc gccttttcgg gttccgtgcg atatttgcag
    17821 ctcaaatcat gacgtggttc atgatcgcga ggacgctgca ccatgccgca cccgaggcaa
    17881 actttaaacc cggttcgctt cttcgcttct ccggcaatca tcaactcatt ggcatcatcc
    17941 gccattttgc cgaagttgat atcccgcagg gtgactttgc ttaaaaattc aaaaccaaac
    18001 ggaacttcgc cttcctcaat cgcgtatgcc gccgatacat cttctttttc gaaactaacc
    18061 aatagctgac gctggaagaa agcaggttca cggctgtcgc tctcgtcgct gatctggcta
    18121 tcacgtgcgc tggcgcgggc atacacctgg cgaagtttca gcagcgtggt tttttgtccg
    18181 gcgtcggccc accccggtgt gccacatttc gggcaatatt tatgctggtc gcccgtctgg
    18241 tcgatatttt cgctgtagtt acagtggcta cagatacgcc agttctccgg ctcagagacc
    18301 ttcaggtcta tctgctcaat ttcaacttta tgcccaccgg catagaaatt attcagcgga
    18361 gccagctcag ccagcgccgt tgatgccggg cgttcatatt cgaaagtggt gttttgatat
    18421 tcacgcgctt ccccgccttc tttacgccgc cagagcacag aacgtaacgt aataccggct
    18481 tccgggaagg cgtagttagg cagcagacct tcatccgtga ggaaattcag cgtctgctta
    18541 ttgttgattt gatttaccag cgccatcagc gcctgccgtt ctgaagttaa ctcgcgcata
    18601 tcgctgtcaa agttttgatc gcgcggcgcg ttctccagct tatcgatact gcgcttgagt
    18661 ttatcgatgc gtgaacgcag agatttgcga tcttccacca gcagttttaa cgcttcttcg
    18721 atacgctgca ccagcgaacg ctgtccaggc gcaccctgaa gataagacaa caactgttgg
    18781 cgagtttctg cggcaagatc ggggaagatc gccgtaaatt gtgcagcaat ctccgcatga
    18841 tgctgttcga tatatctcag taagttatac ggaaaacccg atttacgccc gaactccagc
    18901 tcatccagca tctgtttgac gtttttacag atagcggatt ctggtacacc ggttttcacc
    18961 cagttatcca tacaaaaagc tgccagctgg cgttcgagaa tagccgttgc attgaggaag
    19021 acgccaggag cctgcacctg gccctgcatc atctccagtg gttcttcaaa gaaaaactga
    19081 tcgtgcgggt tcccttctgc cacggtaatg tttaacgcgt taccatcttt acgaccggcg
    19141 cgaccaatac gctgcaagta gttggcctga gcaggtggga cagaacagag taaaacggtg
    19201 gaaagatccc caacgtcgat acccatctcc agcgtcggtg ttgccgacaa caggttgata
    19261 ttccacggtt ggttgccgcg gtagaacgat ttttcggtgg cttcgcgatc ttcacggcta
    19321 agcagccccg tgtgttctgc gccctgcacg cgcgcgatgt ccatgttctc ccaacgccag
    19381 gtcgccgtat tgtcgacttc ctggtaacga ccttcacaac gtaaagagag acaaggcgca
    19441 ttacgccact gccagccttg atcggccgga gcccggacaa cctcacggca acatgagcaa
    19501 ttaagctgaa cgacgtcacg gctcaccacc agcgccgaag gcaccagtcc ccaggcttca
    19561 cgccctttgg tgtcaaaatc ttttactact ccggctattt tcaacgccgt aaacaggcgt
    19621 cgtaacaccg tttccgcaac gctactgata aacagattat tgccgttact cagcgtgcga
    19681 ttgatccaat gctgatacca actggggtta gacttcgcgc caatcaggcg atggaaacca
    19741 ttaatttttt caaacgacgc ataagccggt ttacgcgttt tcggaccgat agaaggcata
    19801 tagcgcgagg tacgctcttt ttgcgtcatc ataaaggtcg tgccatcacg gcgataactt
    19861 tcgaacgccg gatgcataac ggccccggcc tggcgttgat accaaataac ccccagcgcc
    19921 aggtgcgcga cctgctcgac gctcacaccg gacatctcat cacccagctc ttcacaaagc
    19981 tgttgatgca gggcggtaat tgccggatta aagcgttctt tatccactcc ggcaacggca
    20041 taaccggtgg cttccagcgt acgtccgata cgcgcgcgca gggtaaattc gctgtacacc
    20101 tcccagtcga ggcgcaggga aaccagctcc ggcagatcgg agttctccgc cagtttgcct
    20161 tcatttttca gcgtctgata atcattcagc cactgcatat ttggtgcgat gaagttcgct
    20221 gcaaaatgag catcgtccgg tgaacgttca cgcgtttgtt ggcaaacacg cgccgcgaac
    20281 gcatccagcg gtaatgtctc tttttcgcca agaacactgg cgatttgccc acgaatcatc
    20341 agcggccagg ttcgggcagt aaaaaagctg gcacgatgcg cggcgtcttg taccgagtca
    20401 gaaaacgtaa tcagcttttt atgttcgttg aactggctgc cataaagctg gtgaatcatc
    20461 accgccgaca gactggcagc ccgtgacccc aaaatcgaaa tcccctgttt gctgttgcag
    20521 tacgggcatt cattagcaaa ctccagctcg tccttacgct ctttgagcat atccgggatc
    20581 agtacctgca acagctctgt atgaccacaa tgtccacact ggacgctgga ctgtcggttc
    20641 agtttcaggc aggtagggca cagtttctgg tgtgcgccac tgaccgcttt ttcgttgtta
    20701 tccgggaaag cgaggacgat ggagcgccct ttttcaaaga actggcgata aatgccgtcg
    20761 aggtctgttt ccagctgaac gctttgccca tgacgcaggc tggcccagcc ggttgcgtgg
    20821 cagtcacggc agtggataac cggcaaatag tgttcgcggt cttccaccgc cacatcatcg
    20881 gcaaacacca gtcgcggcgt tttcgccacg ctggcgacca gacgacgcat ctcacgaagc
    20941 cacaactggg agcgcagttg cacaaatggc agcaccggac gcactttctt cgcctggaga
    21001 cgtttttgcc gatcgtgttc cgtttccggt acttcgagac gtgcctgcgc gatcagtgcc
    21061 agaatgcttt gcagcacgcg tcgggcatgg tcggcgcttt ctgccagcat aacctggaga
    21121 ttttccagac actctcgttc agagaggatc ccgccttgca gatcatggag taagacatgc
    21181 atcacgctgt ggcggcgtaa cagtgacccc agcgcaatac gcttttcacg cccgtcgtcg
    21241 ctctccagat cgtccgggag ttgcaagtcg ctgtcgggga accacaaagg gatttgcccg
    21301 ttaagatacg ctaccggggt agcgtaactc tgcgggtcca gggccgaagc gacctccggc
    21361 cccggatact gactgtacgc aatggcataa tcctgcaagt actccgctgc cgtgtagcga
    21421 tcttcgcgaa taacggcgtc atcggtaaac ggctggtcaa agattgtttt tgcgtaatcg
    21481 agcagttgcc ccagttcgtc gccgacggta gctgatgtcc cgacgcaggc gaatcgttgg
    21541 ttatccacac ctatgtgatg cttcagacgt cgcaccagac aggccagatc cgacccttgc
    21601 gcgccatcaa acgtatgcaa ttcatccacg accaggtaac gcaatgagcc gggctggttg
    21661 taacgccaga gcggctgatc gccagggcgc atcagcaggt agtcgagcat tttgtagttg
    21721 gtcaacagga tgtccggcgg gctttcgcgc agcgtgtgct tacaggtaat gaccttttct
    21781 gccgacattt tcttgctggg ttcgatttcg ctgtcgccga caaacaagcc gacggtcacc
    21841 ttgccatgca gttgtggatc gctggcgatg gttttggcga aacggctggc ctgatcggtt
    21901 gccagggcgt tcatcgggta gatgataatt gctttaaccc cggcttgtga cgcgcccgcg
    21961 cagtggttga gcagcgggaa cataaaacat tctgttttac cggagccggt accggttgcg
    22021 accagcgtcg gctgtggcgc gtcgcttccc agacgctgaa atgcccgggc ctgatgcgcg
    22081 tgcggcggga acggcagcgt cagattaggg aaaaaattca gcggtaagtc acttttacga
    22141 aacggcaact gggcggaaag gtatggtcct ttcagcagcg tttcgggctg ggccagaaat
    22201 tgctccagca ttgagacatt atcgttattc tcaccgttaa acagcgggct gtttagcggg
    22261 aacgccgccc gtaaaaaggc ggcaacgctg tcggcgacct gccgactgac aacggacggc
    22321 aacatcaggc gctctcctta tcttgtgcaa agaacgccca ggccactttg tagtcgtctt
    22381 cgcgatccgg cttgataaac ggtgcctgcc aggtgacggt acggcgctcg ccttcgtcgc
    22441 tgcgggtata gtcatcaaag gtaacgctga cggtaccggt ttgcagatgt ttgacatcat
    22501 cccagccgat ggcttgatcg gtgcagtcac cgccggtcca gtccgggctg tcgacgttaa
    22561 agacaaagcc gtttttcagg tcagctttac gcgcggtacg aggcaagcca acgcccacca
    22621 gccctttgc
//
LOCUS       DAMZFT010000079        22283 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.079, whole genome shotgun sequence.
ACCESSION   DAMZFT010000079 DAMZFT010000000
VERSION     DAMZFT010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 22283)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22283)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22283
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.079"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..198
                     /locus_tag="PN977_003119"
     CDS             <1..198
                     /locus_tag="PN977_003119"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418096.4"
                     /note="catalyzes the conjugation of cysteine to
                     4'-phosphopantothenate to form
                     4-phosphopantothenoylcysteine, which is then
                     decarboxylated to form 4'-phosphopantotheine; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate synthase"
                     /protein_id="HDI6043129.1"
                     /translation="RIRKNLDLICANDVSQPTQGFNSDNNALHLFWQDGDKVLPLERK
                     ELLGQLLLDEIVTRYDEKNRR"
     gene            176..634
                     /gene="dut"
                     /locus_tag="PN977_003120"
     CDS             176..634
                     /gene="dut"
                     /locus_tag="PN977_003120"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000976070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HDI6043130.1"
                     /translation="MMKKIDVKILDPRVGKEFPLPTYATSGSAGLDLRACLDDAVELA
                     PGDTTLVPTGLAIHIADPSLAAMMLPRSGLGHKHGIVLGNLVGLIDSDYQGQLMISVW
                     NRGQDSFTIQPGERIAQMIFVPVVQAEFNLVEDFDATDRGEGGFGHSGRQ"
     gene            741..1337
                     /gene="slmA"
                     /locus_tag="PN977_003121"
     CDS             741..1337
                     /gene="slmA"
                     /locus_tag="PN977_003121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000818604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion factor SlmA"
                     /protein_id="HDI6043131.1"
                     /translation="MAEKQTAKRNRREEILQSLALMLESSDGSQRITTAKLAASVGVS
                     EAALYRHFPSKTRMFDSLIEFIEDSLITRINLILKDEKDTTARLRLIVLLLLGFGERN
                     PGLTRILTGHALMFEQDRLQGRINQLFERIEAQLRQVLREKRMREGEGYATDETLLAS
                     QILAFCEGMLSRFVRSEFKYRPTDDFDARWPLIAAQLQ"
     gene            complement(1374..2015)
                     /gene="pyrE"
                     /locus_tag="PN977_003122"
     CDS             complement(1374..2015)
                     /gene="pyrE"
                     /locus_tag="PN977_003122"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HDI6043132.1"
                     /translation="MKLYQRQFIEFALSKQVLKFGEFTLKSGRKSPYFFNAGLFNTGR
                     DLALLGRFYAEALVDSGIEFDLLFGPAYKGIPIATTTAVAMAEHHDLDLPYCFNRKEA
                     KDHGEGGNLVGSALQGRVMLVDDVITAGTAIRESMEIIQANGATLAGVLISLDRQERG
                     RGEISAIQEVERDYNCKVISIITLKDLIAYLEEKPEMAEHLAAVKAYREEFGV"
     gene            complement(2081..2797)
                     /gene="rph"
                     /locus_tag="PN977_003123"
     CDS             complement(2081..2797)
                     /gene="rph"
                     /locus_tag="PN977_003123"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006803115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HDI6043133.1"
                     /translation="MRPAGRSNNQVRPVTLTRNYTKHAEGSVLVEFGDTKVLCTASIE
                     EGVPRFLKGQGQGWITAEYGMLPRSTHTRNAREAAKGKQGGRTMEIQRLIARALRAAV
                     DLKALGEFTITLDCDVLQADGGTRTASITGACVALADALQKLVENGKLKTNPMKGMVA
                     AVSVGIVNGEAVCDLEYVEDSAAETDMNVVMTEDGRIIEVQGTAEGEPFTHEELLTLL
                     ALARGGIESIVATQKAALAN"
     gene            2924..3787
                     /locus_tag="PN977_003124"
     CDS             2924..3787
                     /locus_tag="PN977_003124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HDI6043134.1"
                     /translation="MIRSMTAYARREIKGEWGSATWEMRSVNQRYLETYFRLPEQFRS
                     LEPVVRERIRSRLTRGKVECTLRYEPDVSAQGELILNEKLAKQLVTAANWVKMQSDEG
                     EINPVDILRWPGVMAAQEQDLDAIAAEILAALDGTLDDFIIARETEGQALKVLIEQRL
                     EGVSAEVVKVRAHMPEILQWQRERLVAKLEDAQVQLENNRLEQELVLLAQRIDVAEEL
                     DRLEAHVKETYNILKKKEAVGRRLDFMMQEFNRESNTLASKSINAEVTNSAIELKVLI
                     EQMREQIQNIE"
     gene            4009..4833
                     /gene="dinD"
                     /locus_tag="PN977_003125"
     CDS             4009..4833
                     /gene="dinD"
                     /locus_tag="PN977_003125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312547.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein D"
                     /protein_id="HDI6043135.1"
                     /translation="MNEHHQPFEEIRHYGTEGQEFWSARELAPLLDYRDWRNFQKVLA
                     RATQACEASNQAASDHFVETTKMVVLGSGAQRELEDVHLSRYACYLVVQNGDPAKPVI
                     AAGQTYFAIQTRRQELADDEAFRQLREDEKRLFLRNELKEHNKQLVEAAQQAGVATAT
                     DFAIFQNHGYRGLYGGLDQKAIHQRKGLKKSQKILDHMGSTELAANLFRATQTEEKLK
                     RDGVNSKQQANTTHFDVGSKVRQTIQELGGTMPEELPTPQVSIKQLENSVKITEKK"
     gene            5124..5741
                     /locus_tag="PN977_003126"
     CDS             5124..5741
                     /locus_tag="PN977_003126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HDI6043136.1"
                     /translation="MLLHILYLVGITAEAMTGALAAGRRRMDTFGVIIIATATAIGGG
                     SVRDILLGHYPLGWVKHPEYVIIVATAAVLTTIVAPVMPYLRKVFLVLDALGLVVFSI
                     IGAQVALDMGHGPIIAVVAAVTTGVFGGVLRDMFCKRIPLVFQKELYAGVSFASAVLY
                     IALQHYVSNHDVVIISTLVFGFFARLLALRLKLGLPVFYYSHEGH"
     gene            complement(5738..7420)
                     /gene="ligB"
                     /locus_tag="PN977_003127"
     CDS             complement(5738..7420)
                     /gene="ligB"
                     /locus_tag="PN977_003127"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigB"
                     /protein_id="HDI6043137.1"
                     /translation="MKVWMAILISILCWQSSAWAVCPALSPARAQEEISRLQQQIKQW
                     DDDYWKEGKSEVEDGVYDQLSARLTQWQRCFGNETRDAMMPPLNGAVMHPVAHTGVRK
                     MADKNALSLWMRERSDLWVQPKVDGVAVTLVYRDGKLNKAISRGNGLKGEDWTQKVRL
                     ISAVPQTVSGPLANSTLQGEIFLKRKGHIQQQMGGINARAKVAGLMMRQGNSDTLNSL
                     AVFVWAWPDGPQLMTDRLKELATAGFTLTQTYTRAVKNADEVAHVRNEWWKAKLPFVT
                     DGVVVRAAKEPESRHWLPGQAEWLVAWKYQPVAQVAEVKAIQFAVGKSGKISVVASLA
                     PVMLDDKKVQRVNIGSVRRWQEWDIAPGDQILVSLAGQGIPRIDDVVWRGAERTKPTP
                     PENRFNSLTCYFASDVCQEQFISRLVWLGSKQVLGLDGIGEAGWRALHQTHRFEHIFS
                     WLLLTPEQLQNTPGIAKSKSAQLWHQFNLARQQPFTRWVMAMGIPLTRAALNASDERS
                     WSQLLFSTEQFWQQLPGTGSGRARQVIEWKENAQIKKLGSWLAAQQITGFEP"
     gene            7678..8301
                     /gene="gmk"
                     /locus_tag="PN977_003128"
     CDS             7678..8301
                     /gene="gmk"
                     /locus_tag="PN977_003128"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HDI6043138.1"
                     /translation="MAQGTLYIVSAPSGAGKSSLIQALLKTQPLYDTQVSVSHTTRQP
                     RPGEVHGEHYFFVNHDEFKEMISRDAFLEHAEVFGNYYGTSREAIEQVLATGVDVFLD
                     IDWQGAQQIRQKMPHARSIFILPPSKIELDRRLRGRGQDSEEVIAKRMAQAVAEMSHY
                     AEYDYLIVNDDFDTALTDLKTIIRAERLRMSRQKQRHDALISKLLAD"
     gene            8356..8631
                     /gene="rpoZ"
                     /locus_tag="PN977_003129"
     CDS             8356..8631
                     /gene="rpoZ"
                     /locus_tag="PN977_003129"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013511051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HDI6043139.1"
                     /translation="MARVTVQDAVEKIGNRFDLVLVAARRARQMQVGGKDPLVPEEND
                     KTTVIALREIEEGLINNQILDVRERQEQQEQEAAELQAVTAIAEGRR"
     gene            8650..10758
                     /gene="spoT"
                     /locus_tag="PN977_003130"
     CDS             8650..10758
                     /gene="spoT"
                     /locus_tag="PN977_003130"
                     /EC_number="3.1.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional GTP
                     diphosphokinase/guanosine-3',5'-bis pyrophosphate
                     3'-pyrophosphohydrolase"
                     /protein_id="HDI6043140.1"
                     /translation="MYLFESLNQLIQTYLPEDQIKRLRQAYLVARDAHEGQTRSSGEP
                     YITHPVAVACILAEMKLDYETLMAALLHDVIEDTPATYQDMEQLFGKSVAELVEGVSK
                     LDKLKFRDKKEAQAENFRKMIMAMVQDIRVILIKLADRTHNMRTLGSLRPDKRRRIAR
                     ETLEIYSPLAHRLGIHHIKTELEELGFEALYPNRYRVIKEVVKAARGNRKEMIQKILS
                     EIEGRLQEAGIPCRVSGREKHLYSIYCKMVLKEQRFHSIMDIYAFRVIVNDSDTCYRV
                     LGQMHSLYKPRPGRVKDYIAIPKANGYQSLHTSMIGPHGVPVEVQIRTEDMDQMAEMG
                     VAAHWAYKEHGETSTTAQIRAQRWMQSLLELQQSAGSSFEFIESVKSDLFPDEIYVFT
                     PEGRIVELPAGATPVDFAYAVHTDIGHACVGARVDRQPYPLSQPLTSGQTVEIITAPG
                     ARPNAAWLNFVVSSKARAKIRQLLKNLKRDDSVSLGRRLLNHALGGSRKLNEIPQENI
                     QRELDRMKLATLDDLLAEIGLGNAMSVVVAKNLQHGDASIPPATQSHGHLPIKGADGV
                     LITFAKCCRPIPGDPIIAHVSPGKGLVIHHESCRNIRGYQKEPEKFMAVEWDKETAQE
                     FITEIKVEMFNHQGALANLTAAINTTTSNIQSLNTEEKDGRVYSAFIRLTARDRVHLA
                     NIMRKIRVMPDVIKVTRNRN"
     gene            10765..11454
                     /gene="trmH"
                     /locus_tag="PN977_003131"
     CDS             10765..11454
                     /gene="trmH"
                     /locus_tag="PN977_003131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(18)-2'-O)-methyltransferase
                     TrmH"
                     /protein_id="HDI6043141.1"
                     /translation="MNPTRYARICEMLARRQPDLTVCMEQVHKPHNVSAIIRTADAVG
                     VHEVHAVWPGSRMRTMASAAAGSNSWVQVKTHRTIGDAVAHLKGQGMQILATHLSDNA
                     VDFREIDYTRPTCILMGQEKTGITQEALALADRDIIIPMIGMVQSLNVSVASALILYE
                     AQRQRQNAGMYLRENSMLPEAEQQRLLFEGGYPVLAKVAKRKGLPYPHVNQQGEIEAD
                     ADWWATMQAAG"
     gene            11460..13541
                     /gene="recG"
                     /locus_tag="PN977_003132"
     CDS             11460..13541
                     /gene="recG"
                     /locus_tag="PN977_003132"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HDI6043142.1"
                     /translation="MKGRLLDAVPLSSLTGVGAALSNKLAKINLHTVQDLLLHLPLRY
                     EDRTHLYPIGELLPGVYATVEGEVLNCNISFGGRRMMTCQISDGSGILTMRFFNFNAA
                     MKNSLATGRRVLAYGEAKRGKYGAEMIHPEYRVQGDLSTPELQETLTPVYPTTEGVKQ
                     ATLRKLTDQALDLLDTCAIEELLPPELSQGMMTLPEALRTLHRPPPTLQLSDLETGQH
                     PAQRRLILEELLAHNLSMLALRAGAQRFHAQPLSANDALKNKLLAALPFKPTGAQARV
                     AAEIERDMALDVPMMRLVQGDVGSGKTLVAALAALRAIAHGKQVALMAPTELLAEQHA
                     NNFRNWFAPLGIEVGWLAGKQKGKARLAQQEAIASGQVQMIVGTHAIFQEQVQFNGLA
                     LVIIDEQHRFGVHQRLALWEKGQQQGFHPHQLIMTATPIPRTLAMTAYADLDTSVIDE
                     LPPGRTPVTTVAIPDTRRTDIIDRVRHACITEGRQAYWVCTLIEESELLEAQAAEATW
                     EELKLALPELNVGLVHGRMKPADKQAVMASFKQGELHLLVATTVIEVGVDVPNASLMI
                     IENPERLGLAQLHQLRGRVGRGAVASHCVLLYKTPLSKTAQIRLQVLRDSNDGFVIAQ
                     KDLEIRGPGELLGTRQTGNAEFKVADLLRDQAMIPEVQRLARHIHERYPQQAKALIER
                     WMPETERYSNA"
     gene            complement(13575..14780)
                     /gene="gltS"
                     /locus_tag="PN977_003133"
     CDS             complement(13575..14780)
                     /gene="gltS"
                     /locus_tag="PN977_003133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/glutamate symporter"
                     /protein_id="HDI6043143.1"
                     /translation="MFHLDTLATLVAATLTLLLGRKLVHSVSFLKKYTIPEPVAGGLL
                     VALALLILKKSMGWEVNFDMSLRDPLMLAFFATIGLNANIASLRAGGRVVGIFLIVVV
                     GLLVMQNAIGIGMASLLGLDPLMGLLAGSITLSGGHGTGAAWSKLFIERYGFTNATEV
                     AMACATFGLVLGGLIGGPVARYLVKHSTTPNGIPDDQEVPTAFEKPDVGRMITSLVLI
                     ETIALIAICLTVGKIVAQLLAGTAFELPTFVCVLFVGVILSNGLSMMGFYRVFERAVS
                     VLGNVSLSLFLAMALMGLKLWELASLALPMLAILVVQTIFMALYAIFVTWRMMGKNYD
                     AAVLAAGHCGFGLGATPTAIANMQAITERFGPSHMAFLVVPMVGAFFIDIVNALVIKL
                     YLMLPIFAG"
     gene            15060..16451
                     /gene="xanP"
                     /locus_tag="PN977_003134"
     CDS             15060..16451
                     /gene="xanP"
                     /locus_tag="PN977_003134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine/proton symporter XanP"
                     /protein_id="HDI6043144.1"
                     /translation="MSVSTLESENAQPVAQTQNSELIYRLEDRPPLPQTLFAACQHLL
                     AMFVAVITPALLICQALGLPAQDTQHIISMSLFASGVASIIQIKAWGPVGSGLLSIQG
                     TSFNFVAPLIMGGTALKTGGADVPTMMAALFGTLMLASCTEMVISRVLHLARRIITPL
                     VSGVVVMIIGLSLIQVGLTSIGGGYAAMSDNTFGAPKNLLLAGVVLALIILLNRQRNP
                     YLRVASLVIAMAAGYALAWFMDMLPESNEPMTQELIMVPTPLYYGLGIEWSLLLPLML
                     VFMITSLETIGDITATSDVSEQPVSGPLYMKRLKGGVLANGLNSFVSAVFNTFPNSCF
                     GQNNGVIQLTGVASRYVGFVVALMLIVLGLFPAVSGFVQHIPEPVLGGATLVMFGTIA
                     ASGVRIVSREPLNRRAILIIALSLAVGLGVSQQPLILQFAPEWLKNLLSSGIAAGGIT
                     AIVLNLIFPPEKQ"
     gene            16572..18281
                     /locus_tag="PN977_003135"
     CDS             16572..18281
                     /locus_tag="PN977_003135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HDI6043145.1"
                     /translation="MKFIGKLLLYILIALLVVIAGLYFLLQTRWGAEHISAWVSENSD
                     YHLAFGAMDHRFSAPSHIVLENVTFGRDGQPATLVAKSVDIALSSRQLTEPRHVDTIL
                     LENGTLNLTDQTAPLPFKADRLQLRDMAFNSPNSEWKLSAQRVNGGVVPWSPEAGKVL
                     GTKAQIQFSAGSLSLNDVPATNVLIEGSIDNDRVTLTNLGADIARGTLTGNAQRNADG
                     SWQVENLRMADIRLQSEKSLTDFFAPLRSVPSLQIGRLEVIDARLQGPDWAVTDLDLS
                     LRNMTFSKDDWQTQEGKLSMNASEFIYGSLHLFDPIINAEFSPQGVALRQFTSRWEGG
                     MVRTSGNWLRDGKMLILDDAAIAGLEYTLPKNWQQLWMETTPGWLNSLQLKRFSASRN
                     LIIDIDPDFPWQLTALDGYGANLTLVTDHKWGVWSGSANLNAAAATFNRVDVRRPSLA
                     LTANSSTVNISELSAFTEKGILEATASVSQTPQRQTHISLNGRGVPVNILQQWGWPEL
                     PLTGDGNIQLTASGNIQANIPLKPTVSGQLHAVNAAKQQVTQTMNAGVVSSGEVTSTE
                     SVQ"
     gene            complement(18325..19005)
                     /locus_tag="PN977_003136"
     CDS             complement(18325..19005)
                     /locus_tag="PN977_003136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016235022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lyxose/D-mannose family sugar isomerase"
                     /protein_id="HDI6043146.1"
                     /translation="MKRSQINYVIDKAHAIAQTFRVCLPEFAYFTVDDWLQLERDNWQ
                     EVVDLQLGWDITDFGRGDFNQTGLTLLTMRNGALGAAAYPKPYAEKMLQIQQDQQTPW
                     HFHTHKMEDILNRGGGDLCMQLAWANEANLCDDQRVITVSVDGQRRTMKPGETLVLKP
                     GQGICLPPRLYHRFWAEKAFVLGWEISMVNDDQHDNYFLEPGGRFPAIEEDEPVKWLL
                     CSEYGILR"
     gene            complement(18986..19918)
                     /locus_tag="PN977_003137"
     CDS             complement(18986..19918)
                     /locus_tag="PN977_003137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HDI6043147.1"
                     /translation="MHYLGLDIGGTKIAAVVMDAHGWEIRRYRCPTQKSTYQQFVSCV
                     VALIEQIRRDVQRPMLTGIALPGSISPLTGLIKNANIQVINGHALQADLQQLLGQPVV
                     IANDGNCFALSEACDGAGQDYDVVFGITLGSGCGGGIAIKQRPFIGAWGNAAECGHIT
                     LPGYMEQEDGPSVSCYCGKHNCVESFVSGSGFSERYQQMTGNLLTPAAIVTLAQRGDA
                     CAMQQVARFRQQLARTLATIVNVVDPGVIVIGGGLSNVELLIADLNTEVAPLVFTDQF
                     TTPIVKALHGDSSGMRGAAWLAMRNGEANETFTN"
     gene            complement(19966..20826)
                     /locus_tag="PN977_003138"
     CDS             complement(19966..20826)
                     /locus_tag="PN977_003138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006687642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HDI6043148.1"
                     /translation="MLVSMKELLQPTRQHGFAIGAFNVADNCFLRAVVDEAEATNTPA
                     IIAIHPSEHDFVGDAFFAHVREITQRSPVPFVLHLDHGASVEHVLRAIRCGFTSVMID
                     GSLLPYEENVALTAEVVRLAHAVGVSVEGELGTIGQTGTSVEGGVSQVTYTDPAQAAD
                     FIARTGADTLAVAIGTAHGIYPKGMQPKLQMDILRDIAGRIDIPLVLHGGSANPDAEI
                     AESVTLGVGKINISSDMKYAYFQKVREILAKESWWDPNVIYPDAINAAREVIRQKMKL
                     FGSIGKASLY"
     gene            complement(20907..21758)
                     /locus_tag="PN977_003139"
     CDS             complement(20907..21758)
                     /locus_tag="PN977_003139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000457126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase family
                     protein"
                     /protein_id="HDI6043149.1"
                     /translation="MFADMKSMVMKAWHEHYALLAINCMNLESAHAAIRVAEKNRAPI
                     ILNLYQGHLAHFPAPVAAAVVKTLAESASVPVALALDHGKNPDCIRQAFRAGFSGLMI
                     DASAFPLEENVRQTRAVVELAASAQLCVEGELGHLADAPRYDQAANADLMTQPTDVET
                     FIAQTGIDLLAVSVGTAHGMYAPGVVPAIDFQRLAEIFHCSSVPLALHGGSGTPFDQL
                     QLCTTLGVAKINVGAAIFERGKSALLHTLCHDISIELVDALKAMELAFEEAITPYLQA
                     SGSIGKA"
     gene            complement(21770..>22283)
                     /locus_tag="PN977_003140"
     CDS             complement(21770..>22283)
                     /locus_tag="PN977_003140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HDI6043150.1"
                     /translation="GPVNKTMSLFSDGLLVSGVYGPEAVKFVGSIIPPFGITLSFLLT
                     RHKYTRAEREALKAAFPMGICMITEGVIPIAARDLLRVVGSCVVASAVAGGLIMVWGV
                     ESPVPHGGMFVVPLFTHPLLFCLALGIGTVICGVMLSLWKKPVTERDEEFDELSDQKL
                     KDEEITFTLE"
BASE COUNT         5382 a         6079 c         5726 g         5096 t
ORIGIN      
        1 cgtatccgta aaaaccttga tctgatctgc gcgaacgatg tttcccagcc aactcaagga
       61 tttaacagcg acaacaacgc attacacctt ttctggcagg acggagataa agtcttaccg
      121 cttgagcgca aagagctcct tggccaatta ttactcgacg agatcgtgac ccgttatgat
      181 gaaaaaaatc gacgttaaga ttctggaccc gcgcgttggg aaggaatttc cgctcccgac
      241 ttatgccacc tctggctctg ccggacttga cctgcgtgcc tgtctcgacg acgccgtaga
      301 actggctccg ggtgacacta cgctggttcc gaccgggctg gcgattcata ttgccgatcc
      361 ttcactggcg gcaatgatgc tgccgcgctc cggattggga cataagcacg gtatcgtgct
      421 tggtaacctg gtaggattga tcgattctga ctatcagggc cagttgatga tttccgtatg
      481 gaaccgtggt caggacagct tcaccattca acctggcgaa cgcatcgccc agatgatttt
      541 tgttccggta gtacaggctg aatttaatct ggtggaagat ttcgacgcca ccgaccgcgg
      601 tgaaggcggc tttggtcact ctggtcgtca gtaacacata cgcatccgaa taacgtcata
      661 acatagccgc aaacatttcg tttgcggtca tagcgtgggt gccgcctggc aagtgcttat
      721 tttcaggggt attttgtaac atggcagaaa aacaaactgc gaaaaggaac cgtcgcgagg
      781 aaatacttca gtctctggcg ctgatgctgg aatccagcga tggaagccaa cgtatcacga
      841 cggcaaaact ggccgcctct gtcggcgttt ccgaagcggc actgtatcgc cacttcccca
      901 gtaagacccg catgttcgat agcctgattg agtttatcga agatagcctg attactcgca
      961 tcaatctgat tctgaaagat gagaaagaca ccacagcgcg cctgcgtctg attgtgttgc
     1021 tgcttctcgg ttttggtgag cgtaatcctg gcctgacccg catcctcact ggtcatgcac
     1081 taatgtttga acaggatcgc ctgcaagggc gcatcaacca gctattcgag cgtattgaag
     1141 cgcagttgcg tcaggtattg cgtgaaaaga gaatgcgtga gggcgaaggc tacgcgacgg
     1201 atgaaaccct gctggcaagc cagatcctgg ccttctgtga aggtatgctg tcacgctttg
     1261 tccgcagcga gtttaaatac cgcccgacgg atgactttga cgcccgctgg ccgctaattg
     1321 ctgcacagtt gcagtaatat gacgccggat gacttttcat ccggcgagtt tctttaaacg
     1381 ccaaactctt cgcggtaggc cttaaccgcc gccagatgtt ccgccatttc tggcttctct
     1441 tcgaggtaag caatcaggtc tttcagggtg atgatagaga tcactttgca gttgtaatca
     1501 cgctcaactt cctgaattgc cgaaatctcg ccgcgccccc gttcctgacg atcgagcgaa
     1561 atcaatacgc cagcaagcgt cgcgccattg gcctgaataa tttccatcga ctcgcgaatc
     1621 gccgttccgg cggtgatcac atcatccacc agcattacgc gtccttgtaa cgcgctacca
     1681 accagattgc cgccttcacc gtggtctttt gcttctttgc ggttaaagca gtacggcagg
     1741 tccaggtcat gatgctccgc catcgccacg gcagttgtgg tggcaatcgg gatccctttg
     1801 taagcagggc caaacagcag atcgaactca atgccggaat ccaccaatgc ttcagcgtaa
     1861 aaacggccta acagtgccag atcgcgcccg gtattaaaca gcccggcgtt gaagaaatag
     1921 gggcttttgc gcccggattt cagcgtaaac tcgccaaact ttaacacctg cttgctaagc
     1981 gcaaattcaa taaactggcg ctgatatagt ttcatgcctt cgctcctcat cttacttttc
     2041 tacagacaaa aaaaaggcga ctcatcagtc gccttaaaaa tcagtttgcc agcgccgcct
     2101 tctgcgtcgc tacaatggat tcgattcccc ctcgggccag cgccaacaag gtgagcagct
     2161 cttcatgggt gaacggctcg ccttctgccg tcccctgcac ttcaatgatg cgcccgtctt
     2221 cggtcatcac tacgttcatg tcggtctctg cggcagagtc ttcaacgtat tccagatcgc
     2281 aaaccgcttc gccgttcaca atcccgacag aaaccgcggc taccatccct ttcatcgggt
     2341 tggttttcag cttgccgttt tccaccagct tctgtaacgc atctgccagc gccacgcagg
     2401 cacccgtaat cgaagcggta cgcgtgccac catcagcctg aagcacgtcg cagtccagcg
     2461 taatagtgaa ctcacccagc gctttcaaat ctactgctgc gcgaagagca cgggcgatca
     2521 gacgctggat ttccattgtg cgcccaccct gcttaccttt cgctgcttca cgcgcgttac
     2581 gggtgtgggt agaacgtggc agcatgccgt actctgcggt gatccagccc tggccctgac
     2641 ctttcaggaa gcgcggcacg ccttcttcaa tagaggcggt acacaatact ttggtatcgc
     2701 caaattcgac cagcaccgag ccttctgcat gttttgtata gttacgagtc agggtaacgg
     2761 gacgcacctg attattgcta cggcctgctg gacgcatatt gaaatctccg gcttgaaaca
     2821 aatgtggctg cgcattatac ggacttccgg cggttattcc tatcctgaca aggcatcgat
     2881 ggctataatc cttccacctc tccttttata aacaggaacg tctatgatcc gcagtatgac
     2941 cgcctacgcc cggcgtgaaa tcaagggtga atgggggagc gcaacctggg aaatgcgctc
     3001 ggtaaaccag cgttatctgg aaacttactt tcgtctgccg gagcagttcc gtagccttga
     3061 acctgtcgtt cgtgagcgta ttcgttctcg cctgacgcgc ggtaaagtgg aatgtaccct
     3121 gcgctatgag ccagatgtta gcgcgcaagg tgagctgatc ctcaacgaaa aactggctaa
     3181 acagctggta actgccgcga actgggtaaa aatgcagagt gacgaagggg aaatcaatcc
     3241 ggttgatatt ctacgctggc cgggcgtgat ggcagcccag gagcaggatc tcgacgcgat
     3301 tgccgctgaa attctcgcgg cgttggatgg tacgctggac gactttatca tcgcacgtga
     3361 aaccgaaggt caggcgctga aagtgttgat tgagcagcgt ctggaaggcg tcagcgccga
     3421 agtggtcaaa gtccgcgccc atatgccgga aatcctgcaa tggcagcgtg agcgtctggt
     3481 cgcgaagctg gaagatgctc aggtgcaact ggaaaacaac cgtctggagc aggagttggt
     3541 tctgctggca caacgaattg acgttgccga agaactggat cgcctcgaag cgcatgtcaa
     3601 agagacctac aacattctga agaaaaaaga agcggttggc cgtcgtctgg actttatgat
     3661 gcaggagttc aaccgcgagt cgaacactct tgcttcgaag tctatcaatg ccgaagtgac
     3721 aaactccgcg attgagctga aagtgttgat tgagcagatg cgcgagcaga ttcagaacat
     3781 tgaataactc gtaacgccaa ttcttccttt tcagccttca caaatgccgc ccactcaaac
     3841 agagcggcat ttttcctccc cgcaacattc aattctgttt tgcgtgcctg ctccagattt
     3901 tgcgatgttt ttttgcccag cgcagtgaga acgtgagata ctcacaactg tatataaaca
     3961 cagttgtaaa cttactttcc ttgcaatcga tatcacatgg agtgggcaat gaacgaacat
     4021 catcaacctt ttgaagaaat acggcattat ggcactgaag ggcaagaatt ttggtcagcg
     4081 agagaattgg ctccgttact ggattatcgg gactggcgca attttcaaaa ggtactggcc
     4141 cgcgcgactc aagcgtgcga agccagcaat caagctgcat ctgaccattt cgttgaaacc
     4201 accaaaatgg ttgtattagg ttcaggggct caacgagaac ttgaagatgt tcatctctcc
     4261 cgctatgcct gttacctggt agtacaaaac ggcgaccctg cgaaaccggt tattgcggca
     4321 gggcaaactt attttgctat ccagacccga cggcaggagc tggctgacga tgaagctttc
     4381 aggcaacttc gtgaagacga aaagcgtctt tttctgagaa atgagttgaa agaacataac
     4441 aaacaattag ttgaggcagc gcagcaagct ggggtagcga cagctaccga tttcgccata
     4501 tttcagaatc atggttaccg gggactgtat ggtggattag atcagaaagc catccatcag
     4561 cggaaggggc tgaaaaagag tcagaagatc ctggatcata tgggttcaac agaactggcg
     4621 gctaatctct ttcgagctac ccaaacagaa gaaaaactca agcgggatgg cgtgaattca
     4681 aaacagcaag ctaataccac acactttgac gtgggtagca aggtgaggca aaccattcag
     4741 gaacttggcg gaaccatgcc tgaagagttg ccgaccccgc aggtcagcat caagcagttg
     4801 gaaaacagtg taaaaattac agagaagaaa tagcagaaac taaaaactct ttttgttgat
     4861 tgagataccc gatgcgtaag ccaaagtcca ggtgcggtga acacaatggc taaacattgc
     4921 acctttcttt tccaccctca attttaacct attttttctt atgcattttc tcagacaaga
     4981 agtcagaaga atacatctac gctacagaaa atagcgattt cacataacta aaagttatca
     5041 atttcctctc accttaaaaa atctcaatcg tgacaatacg cacaaatcgc taccctgcca
     5101 gacagatttt tagggagaga accatgctgt tacacatttt gtatttggtt ggcattactg
     5161 ccgaagccat gacgggggcg ctggcggccg gacgacggcg catggataca tttggcgtaa
     5221 ttattattgc gaccgccacc gcaattggcg gagggtcagt gcgcgatatt ctgctgggcc
     5281 actatccgct cggctgggtc aaacacccgg aatatgtgat tattgtcgct accgccgcag
     5341 tactcaccac tatcgttgct cctgtaatgc cttacctgcg caaagtgttt ctggtgctcg
     5401 atgcgctcgg actggtagtc ttttctatca ttggcgcaca ggttgcactg gatatgggac
     5461 acggcccgat cattgccgtt gtcgcagctg tgacgaccgg cgtatttggc ggcgttttgc
     5521 gcgatatgtt ctgtaaacgc atcccactgg tattccagaa agagttgtat gccggtgtct
     5581 catttgcctc cgccgtgctg tacatcgcgc tgcaacatta tgtttctaac catgatgtgg
     5641 tgattatctc caccctggta ttcggctttt ttgcccgctt actggcgcta cgtcttaagc
     5701 tgggattacc ggtcttttac tacagccacg aaggtcacta aggttcaaaa cctgtgatct
     5761 gctgggcagc cagccaactg cccagcttct tgatttgcgc attttccttc cattcaataa
     5821 cctgtctggc gcgccccgat ccagtccccg gcaactgctg ccagaactgt tctgtgctaa
     5881 ataaaagttg cgaccaggac cgttcatcac tggcattaag cgccgcccgg gttagcggta
     5941 ttcccattgc catcacccag cgagtaaaag gctgctgacg agccagatta aattgatgcc
     6001 ataactgcgc acttttactt ttcgcgatcc ccggcgtgtt ctgtaattgc tctggcgtta
     6061 ataaaagcca ggaaaagata tgctcaaagc gatgagtctg atgcagcgcg cgccagccgg
     6121 cctcaccaat gccatccagc ccaagaacct gttttgaccc cagccagact aagcgtgaaa
     6181 tgaactgttc ctgacaaaca tcagaagcaa agtagcaggt caacgagtta aagcggtttt
     6241 ctggtggtgt cggttttgta cgttctgcac cgcgccagac cacatcatca atgcgaggaa
     6301 taccctgacc ggcaaggctg acgaggatct gatcaccagg cgcaatatcc cactcctgcc
     6361 agcgcctgac ggaaccaata ttcacccgct ggactttttt atcatccagc atgacaggtg
     6421 cgagtgacgc aaccaccgat attttaccgc tcttacccac cgcaaactga atcgccttca
     6481 cttcggcaac ctgagctaca ggttgatatt tccaggccac cagccactcc gcctggcccg
     6541 gtagccaatg acgggattcg ggttctttcg ccgctcgcac aaccacgcca tcggtgacga
     6601 agggtaattt tgctttccac cactcattgc gtacgtgcgc aacctcatca gcatttttaa
     6661 ccgcacgggt atacgtctgc gttagagtaa aacctgcggt agccagctcc tttaaacgat
     6721 cggtcattaa ctgcggtcca tccggccatg cccagacaaa aacagccagt gagtttagcg
     6781 tatcgctatt cccctggcgc atcatcaggc cagcaacttt tgcgcgggca tttattcccc
     6841 ccatttgttg ttggatatgc cccttacgct tgagaaatat ttccccctga agcgtgctgt
     6901 tggctaaagg cccgctaacg gtttgcggca cagcggaaat taagcgaact ttctgcgtcc
     6961 agtcctcgcc tttcaggcca ttaccgcgac tgattgcttt gttcagtttc ccgtcccgat
     7021 aaaccagggt taccgcaacg ccatcaactt ttggctgcac ccaaagatcg ctacgttcgc
     7081 gcatccacag acttaatgcg tttttatccg ccattttgcg tacacccgta tgggcaaccg
     7141 gatgcataac cgcaccgttt aaaggcggca tcatagcgtc gcgagtctcg ttcccaaaac
     7201 agcgttgcca ctgcgtgaga cgggcgctta actgatcgta aacaccgtct tccacctcac
     7261 ttttcccttc cttccagtag tcatcgtccc actgttttat ttgctgttgc aggcgggaaa
     7321 tttcttcctg tgctctggct ggcgacaagg ccggacagac tgcccacgcc gatgattgcc
     7381 agcacaagat acttattaat atcgccatcc atactttcat catcacctcc gctgtagata
     7441 gtcaggcaga tatacaacgt gatgaaagca aagccgagtg gcaaaaacgg agtctgcgag
     7501 gacgcttcct gagaatcgtc tttattgcag tgaatgacag gcaaatgcgg aagcagctac
     7561 gcaaaacgca acaactttgc gcaaaaagtg tgagcaaggg ctacgtcaca tgcccgcgcc
     7621 gtgtataata agctcgtatg taggctttat ttcgctaatc acatacgaaa gatactcatg
     7681 gctcaaggca cgctttatat tgtttctgcc cccagtggcg cgggtaaatc cagcctgatt
     7741 caggctttat taaaaaccca accgttgtat gacacccagg tttctgtttc acacaccaca
     7801 cgccaaccgc gtcctggtga agtccacggt gaacattatt tctttgttaa tcatgatgaa
     7861 tttaaagaaa tgattagcag agatgcgttc ctcgaacacg cagaagtttt tggtaattac
     7921 tatggcactt cgcgtgaggc cattgagcaa gtactggcga ccggtgtcga tgtttttctc
     7981 gatatcgact ggcagggtgc gcagcaaatt cgccagaaga tgccgcacgc gcggagtatc
     8041 tttattttac cgccgtccaa aattgaactg gaccgccgtc tacgcggtcg cggtcaggac
     8101 agcgaagagg tcattgcaaa gcgtatggcg caagctgttg cagaaatgag ccattacgcc
     8161 gaatatgatt acctgattgt gaatgatgac ttcgataccg cgttgaccga tttgaagacc
     8221 attattcgcg ccgaacgtct gcgcatgagc cgccaaaagc agcgtcatga cgctttaatc
     8281 agcaaattgt tggcagactg aacctgattt cagtatcatg cccagtcatt tcttcacctg
     8341 tggagctttt taagtatggc acgcgtaact gttcaggacg ctgtagagaa aattggtaac
     8401 cgttttgacc tggtactggt cgccgcgcgt cgcgctcgtc agatgcaggt aggcggaaag
     8461 gatccgctgg taccggaaga aaacgataaa accactgtaa tcgcgctgcg cgaaatcgaa
     8521 gaaggtctga tcaacaacca gatcctcgac gttcgtgaac gccaggaaca gcaagagcag
     8581 gaagccgctg aattacaagc cgttaccgct attgctgaag gtcgtcgtta atcacaaagc
     8641 gggtcgccct tgtatctgtt tgaaagcctg aatcaactga ttcaaaccta cctgccggaa
     8701 gaccaaatca agcgtctgcg gcaggcgtat ctcgttgcac gtgatgctca cgaggggcaa
     8761 acacgttcaa gcggtgaacc ctatatcacg cacccggtgg cggttgcctg cattctggcc
     8821 gagatgaaac tcgactatga aacgctgatg gcggcgctgc tgcatgacgt gattgaagat
     8881 actcccgcca cctaccagga catggaacag ctttttggta aaagcgtcgc cgagctggta
     8941 gagggggtgt cgaaacttga taagctcaag ttccgcgata agaaagaggc gcaggccgaa
     9001 aactttcgca agatgattat ggcgatggtg caggatatcc gcgtcattct catcaaactt
     9061 gccgaccgta cccacaacat gcgcacgctg ggctcacttc gcccggacaa acgtcgccgc
     9121 atcgcccgtg aaacactcga aatttacagc ccgctggcgc accgtttagg tatccaccac
     9181 attaaaaccg aactcgaaga gctgggtttt gaggcgctgt atcccaaccg ttaccgcgta
     9241 atcaaagaag tggtgaaagc cgcacgcggc aaccgtaaag agatgatcca aaaaatcctc
     9301 tctgaaatcg aagggcgttt gcaggaagcg ggaataccgt gccgcgtcag tggtcgcgag
     9361 aagcatcttt attcgatcta ctgcaaaatg gtgctcaaag agcagcgttt tcactcaatc
     9421 atggacatct acgctttccg cgtgatcgtc aatgattccg acacctgtta tcgcgtgctc
     9481 gggcaaatgc atagcctgta caagccgcgt ccgggtcgcg tgaaagacta catcgccatt
     9541 ccaaaagcga acggctatca gtctttgcac acctcgatga ttggcccgca cggcgtgccg
     9601 gttgaggtcc aaatccgtac cgaagatatg gatcagatgg cggagatggg tgttgccgcg
     9661 cactgggctt ataaagagca cggcgaaacc agcaccaccg cgcaaatccg cgcccagcgc
     9721 tggatgcaaa gcctgctgga gctgcaacag agtgccggta gctcgtttga atttatcgag
     9781 agcgttaaat ccgatctctt cccggatgag atttacgttt tcacaccgga agggcgcatt
     9841 gtcgaactgc ctgccggtgc aacgcccgtc gacttcgctt atgcggtgca taccgatatc
     9901 ggtcatgcct gcgtgggcgc gcgtgtcgac cgtcagcctt acccgctgtc gcagccgctt
     9961 accagcggtc aaaccgttga aatcattacc gcaccgggcg ctcgcccgaa tgccgcgtgg
    10021 ctgaactttg tcgtcagctc gaaagcgcgc gccaaaattc gtcagttgct gaaaaacctc
    10081 aagcgtgatg attctgtaag cctgggacgt cgtctgctca accatgcgtt gggtggtagc
    10141 cgtaagctca atgaaatccc gcaggaaaat attcagcgcg agctggaccg catgaagctg
    10201 gcaacgcttg acgatctgct ggctgaaatc ggccttggta acgcaatgag cgtggtggtc
    10261 gcgaaaaatc tgcaacatgg ggacgcctcc attccaccgg caacccaaag tcacggacat
    10321 ctgcccatta aaggtgccga tggcgtgctg atcacctttg cgaaatgctg ccgccctatt
    10381 cctggcgacc cgattatcgc ccacgtgagc cccggtaaag gtctggtgat ccaccatgaa
    10441 tcctgccgta acatccgtgg ctaccagaaa gagccagaga agtttatggc tgtggaatgg
    10501 gataaagaga cggcgcagga gttcatcacc gaaatcaagg tggagatgtt caatcatcag
    10561 ggcgcgctgg caaacctgac agcggcaatt aacaccacga cctcgaatat tcaaagtttg
    10621 aatacggaag agaaagatgg tcgcgtctac agcgccttta ttcgtctgac cgcccgtgac
    10681 cgtgtgcatc tggcgaatat catgcgcaaa atccgcgtga tgccagacgt gattaaagtc
    10741 acccgaaacc gaaattaatg ttttatgaac ccaacacgtt atgcacgtat ctgcgaaatg
    10801 ctcgccaggc ggcagcctga tctgaccgtc tgcatggagc aggtccacaa acctcataac
    10861 gtttctgcga ttattcgtac cgcagatgcc gttggtgtac atgaagttca tgctgtctgg
    10921 cctggtagcc ggatgcgcac catggcttcg gcagctgcgg gtagtaacag ctgggtacag
    10981 gtgaaaacgc accgcaccat tggcgatgcc gtcgctcatc tgaaaggcca gggcatgcag
    11041 attctggcaa cccatctttc tgataacgct gtcgatttcc gcgaaattga ttacactcgc
    11101 ccgacctgta ttttgatggg acaggagaaa acaggcatca cgcaggaagc gttggccctg
    11161 gcggatcggg acatcatcat tccgatgatt ggcatggtgc aatcgctgaa tgtttccgtt
    11221 gcctcagccc tcattcttta cgaagcccag cgccagcggc aaaatgcggg catgtacctg
    11281 cgtgaaaaca gcatgttgcc ggaagcagag caacaacgcc tgttgtttga aggcggctat
    11341 ccggtgctgg cgaaagtcgc aaaacgcaaa ggcctgcctt acccccacgt caatcagcag
    11401 ggcgaaattg aagctgatgc cgactggtgg gctaccatgc aggctgcagg ataagtgcca
    11461 tgaaaggtcg cctgttagat gctgtcccgc tcagttccct aacgggcgtt ggcgcagcgc
    11521 ttagtaacaa gctggcgaaa atcaacctgc ataccgtgca ggatctactc ttacaccttc
    11581 ccctgcgcta cgaagatcgc acccatctct accccatcgg agaactacta ccgggcgttt
    11641 atgccacggt ggaaggcgaa gtgctgaact gcaatatctc cttcggcggt cggcggatga
    11701 tgacctgcca gatcagcgac ggttccggca ttctcaccat gcgctttttc aacttcaacg
    11761 cggcaatgaa aaatagcctg gcgacgggcc gccgcgtgct ggcctatgga gaagcaaagc
    11821 gcggtaaata tggtgcggag atgatccacc cggaataccg cgtgcagggc gatctcagta
    11881 cgccagagtt gcaggaaacg cttacgccgg tttatccaac aaccgaaggc gtaaagcagg
    11941 ccacgctgcg taaattaacc gaccaggcgc ttgatctgct cgacacctgc gccattgaag
    12001 aactcctgcc gccggaactg tcacaaggaa tgatgacgct accggaagcg ttgcgcactt
    12061 tgcaccgccc gccgccgacg ctacagctta gcgatctgga aaccggccag catccggcac
    12121 aacgccgtct gattctggag gaactgctgg cgcacaacct cagcatgtta gccttacgtg
    12181 ccggagcaca gcgttttcat gcccagccac tgagcgccaa tgacgcgctg aaaaataaac
    12241 tcctcgccgc cttaccgttc aagccaacgg gcgcacaggc acgcgtagcg gcggagatcg
    12301 agcgcgatat ggcgctggat gtgccgatga tgcgcctggt acagggcgat gtaggttccg
    12361 gtaaaacgct ggtcgccgcc ctcgccgcgc tgcgtgcgat tgcccacggc aaacaggtgg
    12421 cattgatggc accaaccgaa ttactcgccg aacagcacgc caataacttc cgtaactggt
    12481 ttgcaccgct cggtatcgaa gtgggctggc tcgccggtaa gcagaaaggt aaagcgcggc
    12541 tggcacagca ggaagccatc gccagcggtc aggtgcagat gattgtcgga acacacgcca
    12601 tcttccagga acaggtgcag tttaacggtc tggcgctggt gattatcgac gaacagcatc
    12661 gttttggcgt gcatcagcgt ctggcgttat gggagaaagg ccagcagcag ggcttccatc
    12721 cgcatcagtt gatcatgacc gccacgccca tcccccgcac gctggcgatg actgcgtatg
    12781 ccgatctcga tacctcggtg atagatgagc tgccgccagg acgcacgcca gtgactacgg
    12841 tcgctattcc tgatacccgc cgtaccgaca ttattgaccg cgtgcgccac gcctgcataa
    12901 ctgaaggtcg tcaggcatac tgggtttgta cgttgattga agagtcggaa ttgctggaag
    12961 cgcaggcggc ggaagcaacc tgggaagagt tgaaactggc gttgccggaa ctgaacgtcg
    13021 gcctggtaca cgggcggatg aaacctgccg ataaacaggc ggtgatggcg tcgtttaaac
    13081 aaggtgaact acacctgctg gttgccacta ccgttattga agtcggtgtt gatgtgccta
    13141 acgccagtct gatgattatc gaaaacccgg agcgtctggg tctggcgcag ttacaccagc
    13201 ttcgcgggcg tgtaggtcgt ggtgcggtgg cttctcactg cgtgctgctc tacaaaacgc
    13261 cgctctccaa aaccgcgcaa attcggctgc aagtgctgcg cgacagcaac gacggttttg
    13321 tgattgcaca gaaagactta gagattcgcg gaccgggtga actgttaggt acgcgtcaga
    13381 cgggtaatgc tgaatttaaa gtggcggatt tactgcgcga tcaggcgatg atcccggaag
    13441 ttcagcgcct ggcgcgacat attcacgaac gttacccaca acaggcaaaa gccctgatag
    13501 aacgctggat gccggagacg gaacgttact cgaatgcgta aaaggcggcg gtgagaatac
    13561 cgccgcttca tcggttaacc ggcaaaaatc ggcaacatca aatacagctt aatcaccagc
    13621 gcattgacga tatcgataaa gaacgcaccg accatcggca ctaccaggaa cgccatgtgc
    13681 gacgggccaa agcgttcagt gatcgcctgc atgttggcga ttgctgttgg cgttgcgcca
    13741 aggccaaagc cacagtgacc cgcagccagc actgccgcat cgtagttttt gcccatcatg
    13801 cgccaggtaa cgaagatggc atacaacgcc atgaagatgg tctgtaccac cagaatcgcc
    13861 agcatcggca gcgccagcga agccagctcc cacagtttca gccccatcaa cgccatcgcc
    13921 aggaacaacg acaggcttac gttacccagc acggataccg ctcgctcaaa gacgcggtaa
    13981 aagcccatca ttgacagacc gttgctcaga atcacgccaa caaatagtac acagacgaag
    14041 gtcggtagtt caaaagcagt accagccaaa agttgcgcaa caattttccc caccgtcagg
    14101 cagatagcaa tcagcgcgat agtttcaatc aacaccagcg aggtgatcat gcggcccaca
    14161 tccggctttt cgaacgcggt cggaacttcc tggtcatccg gaataccgtt cggtgtggtg
    14221 gagtgtttca ccagatagcg tgccaccgga ccgccaatca agccgcccag caccaaaccg
    14281 aacgttgcac aggccatcgc cacttctgtc gcattggtga agccataacg ttcaatgaac
    14341 aatttactcc atgcagctcc cgtaccgtga ccgccggaaa gcgtaataga accggccaac
    14401 agccccatca gcggatcaag ccctaacagg ctagccatac caatgccaat ggcattttgc
    14461 atcaccaaca ggccaacaac cacaatcaag aagatgccaa ccacacgccc accggcacgc
    14521 aaactggcaa tgttggcgtt caggccaatg gtggcgaaga aagccagcat caacggatcg
    14581 cgcagggaca tatcaaagtt gacttcccag cccatgcttt ttttcagtat cagcagcgcc
    14641 agcgccacta ataaaccacc cgcaacaggt tccggtatgg tgtatttctt caaaaaggag
    14701 acggaatgga ccaacttgcg cccgagcagc aacgtcagcg ttgcggcaac aagcgttgct
    14761 aaagtatcga gatgaaacat agttactcct ttgtgtccgc atcactcttt catactcgtc
    14821 atacttcatc gcgcatttgc agtaaggcga aatatgacga gtatcgagtc atgctttcct
    14881 ggttcgaatt cttcgctgaa aatagaaaaa tggcatggat tttaagcaga aaacaactaa
    14941 aagttatata aaaaagtaca tttatctcat ttaaatattt aaacgaggat gtagcaaacg
    15001 tttgcttttc tctttcggtc agctaaaatg cccgctttgc taccacggga ttgttttcga
    15061 tgtctgtttc caccctcgag tcagaaaatg cacaaccggt tgcgcagact caaaacagcg
    15121 aactgattta ccgtcttgaa gatcgtccgc cgcttcctca aaccctgttt gccgcatgtc
    15181 agcatttgct ggcgatgttc gttgcggtga tcacgccagc gctattaatc tgccaggcgc
    15241 tgggtttacc ggcacaagac acgcaacaca ttattagtat gtcgctgttt gcctccggtg
    15301 tggcatcgat tattcaaatt aaggcctggg gtccggttgg ctccgggttg ttgtctattc
    15361 agggcaccag cttcaacttt gttgctccgc tgattatggg cggcacagcg ctgaagaccg
    15421 gtggtgctga tgttcctacc atgatggcgg ctttgttcgg cacgttgatg ctggcaagtt
    15481 gtaccgagat ggtgatctcc cgcgttctgc atctggcgcg ccgcattatt acgccgctgg
    15541 tttctggcgt tgtggtgatg attatcggcc tgtcgctaat tcaggtgggg ctgacctcca
    15601 ttggcggcgg ttacgcagcc atgagcgata acaccttcgg cgcaccgaaa aatctgctgc
    15661 tggcaggcgt ggtcttagcc ttaattatcc tgcttaaccg tcaacgtaac ccttacttac
    15721 gcgtggcctc actggtgatt gcgatggcgg ccggatatgc gctggcgtgg tttatggaca
    15781 tgttgccaga aagcaacgaa ccgatgacgc aagaactgat tatggtgcca acgccgctct
    15841 attacggtct tggcattgaa tggagtctgc tgctgccgct gatgctggtc tttatgatca
    15901 cttcgctgga aaccattggc gatatcacgg cgacctctga cgtttccgaa cagccggttt
    15961 ccggtccgct gtacatgaaa cgcctgaaag gcggcgtgct ggcaaacggc ctgaactcgt
    16021 ttgtctcggc ggtatttaat accttcccga actcctgctt cgggcagaac aacggggtga
    16081 tccagttaac cggtgttgcc agccgctatg tcggttttgt cgtcgcgctg atgttgatcg
    16141 tgctgggtct gttcccggca gtgagcggtt ttgtgcaaca cattccagaa ccggttctgg
    16201 gcggcgcaac gcttgtaatg tttggcacca tcgctgcctc cggtgtgcgc attgtgtctc
    16261 gtgagccgct gaaccgtcgg gcgattctga ttatcgcgct gtcgctggcg gttggtctgg
    16321 gcgtgtctca gcagccgctg attttgcagt ttgcccctga atggctgaaa aacctgctct
    16381 cctccgggat cgccgcgggc ggtattactg ccatcgtgct gaatctgatt ttcccaccag
    16441 aaaaacagta attcactggc ggcggtaaga taattaccgc cgcagtctca cctctttcac
    16501 cattcccgcc ttgaggattg cgcgtaaatc gtgcataact cccttatgtg catttcacgg
    16561 gatggaagac catgaaattt attgggaagc tgcttctcta cattctcatc gctctgttag
    16621 tggtgatcgc tggcctctat tttctgctgc aaactcgctg gggagcagaa catatcagcg
    16681 catgggtttc cgagaatagc gactatcatc tggccttcgg ggcgatggat caccgttttt
    16741 ccgcgccatc tcatatcgtg ctggagaacg tcacgtttgg ccgtgatggc cagcccgcga
    16801 ccctggtggc aaaaagtgtc gacattgcgc taagcagtcg gcaactgacc gaaccacgcc
    16861 atgtcgatac catcctgctg gaaaacggga cgctgaatct caccgaccag accgcgccgc
    16921 taccgttcaa agccgatcgt ctgcaactac gtgatatggc gtttaatagc ccgaatagcg
    16981 aatggaaact gagcgcgcag cgggtaaatg gcggcgtggt tccttggtca ccagaagccg
    17041 gtaaagtgct gggtacgaag gcgcagattc agtttagtgc cggatcgctt tcgctcaatg
    17101 atgttcctgc caccaatgta ctgattgaag gcagtattga taacgatcgc gttacgctga
    17161 ctaacctggg tgccgacatc gcccgcggga cattaaccgg aaacgcgcag cgtaacgctg
    17221 acggcagctg gcaagtggaa aacctgcgca tggcggatat ccgtctacaa agcgaaaaat
    17281 cgctaaccga cttctttgcg ccattacgct ctgtcccgtc gttgcagatt ggtcgcctgg
    17341 aagtcatcga cgctcgtttg caaggtccgg actgggcggt gaccgacctc gatctcagct
    17401 tgcgcaacat gaccttcagt aaagatgact ggcagacaca ggaaggtaaa ctgtcgatga
    17461 acgccagcga gtttatttat ggctcgctgc atttatttga tccgattata aacgcggaat
    17521 tttccccgca gggcgtagca ctgcgccagt tcaccagccg ctgggaaggg ggcatggtca
    17581 gaacgtcagg gaactggttg cgtgacggga aaatgttgat cctcgatgat gcggcaattg
    17641 ccggactgga atataccttg ccaaaaaact ggcaacagtt gtggatggaa acgacacccg
    17701 gttggttaaa cagcctgcaa ctgaagagat ttagcgccag ccgcaatctg atcattgata
    17761 tcgaccctga cttcccgtgg cagctcaccg cgctcgatgg ttacggtgcc aacctgacgc
    17821 tggttaccga tcataaatgg ggcgtctgga gtggttcggc gaatctgaat gccgccgccg
    17881 cgacattcaa tcgtgttgat gttcgtcgcc catcgctggc gctgaccgcc aacagcagca
    17941 cggtgaatat cagcgaactg agtgcattta ctgaaaaagg cattctggaa gccaccgcca
    18001 gtgtttcaca aacgccacaa cgtcagaccc atatcagcct gaatggacgc ggtgtgccgg
    18061 tgaatatttt gcaacagtgg ggatggcctg aattaccgtt gactggcgac ggcaatattc
    18121 agcttaccgc cagcggcaat attcaggcca atatcccgct gaaacctacg gttagcgggc
    18181 aattgcatgc cgtgaatgcc gcaaagcagc aagtgactca aaccatgaat gcgggcgtag
    18241 tttccagtgg tgaagttacg tcgacggagt cggtgcagta aaaataagga cgctagcgga
    18301 tatcagcgct agcgttcccc gttattagcg caaaattcca tactcactgc acaataacca
    18361 tttcaccggt tcatcttctt ctatcgccgg aaaacgtcct cccggctcaa gaaaatagtt
    18421 atcgtgttgg tcatcattta ccatcgagat ttcccagccg agaacaaacg ctttttccgc
    18481 ccagaagcga tgatacagac gcggcggcag gcaaatgccc tgccccggct ttagcaccag
    18541 cgtttcacca ggtttcatgg tacgccgctg accatcaaca ctcacggtaa tgactcgttg
    18601 atcatcacag agattggctt cgttggccca ggccaactgc atgcataagt ctccaccgcc
    18661 ccgattgagg atatcttcca ttttgtgggt gtgaaaatgc cagggagttt gctgatcttg
    18721 ttggatctgc aacattttct cagcatacgg ttttggatat gccgccgccc ccagcgcgcc
    18781 gttgcgcatc gttagcagtg ttagtcccgt ttggttgaaa tcaccacgac caaaatcggt
    18841 gatatcccaa ccgagctgta agtcgaccac ttcttgccag ttatcccgtt ccagctgcag
    18901 ccagtcatcc accgtaaaat aggcgaactc tggcaaacaa acgcggaagg tctgcgcaat
    18961 ggcatgtgct ttatcaataa cataattaat ttgtgaacgt ttcattggct tctccgttgc
    19021 gcatagcaag ccaggcagcg ccacgcattc cgctactgtc gccgtgcagt gcttttacaa
    19081 tgggggtggt gaattggtcg gtgaaaacca gaggagcgac ttctgtgttc agatcggcga
    19141 taagcagttc cacattcgaa agcccgccgc cgatgacaat cacgccaggg tcaacaacgt
    19201 taacgatggt tgccagcgtg cgggcaagct gttggcgaaa acgtgccacc tgctgcatgg
    19261 cacaagcatc accacgttgt gccagggtga caatcgccgc aggagtgagc aagttaccag
    19321 tcatctgttg atagcgttca ctaaaaccgc tgccggaaac aaacgactcc acgcagttgt
    19381 gtttgccgca atagcaactg actgatggac catcttcctg ctccatatag cctggcaacg
    19441 tgatatgacc gcattcggca gcatttcccc aggcccctat aaacggtcgt tgcttgatgg
    19501 caatgccacc gccgcagccc gaaccaagcg taataccaaa taccacgtca taatcttgcc
    19561 cggcaccgtc gcaagcttct gatagcgcga aacagttacc atcattggct atcaccaccg
    19621 gttgcccaag caattgctgc aaatcagcct gtaacgcatg accgttaatc acctgaatat
    19681 tcgcgttttt aatcaggcca gtgagtggcg agatactgcc gggtaaggcg atccctgtca
    19741 gcatcggtcg ttgaacgtcc cgcctaatct gctcgataag cgccacaacg catgagacaa
    19801 attgttgata tgtcgacttt tgcgtcgggc agcggtaacg gcgaatctcc cagccatgcg
    19861 catccatgac gacggcggcg attttggtcc caccaatatc caaccccaga tagtgcatat
    19921 ctcgttcctt gttgcgtaaa aggcgggttg ccccgccctt ctggcttagt acagtgacgc
    19981 tttaccgatc gaaccgaaca gtttcatttt ctggcgaatg acttcccgcg ccgcgttaat
    20041 cgcatccgga tagatgacgt tcgggtccca ccagctctct ttagcgagaa tttcacgcac
    20101 tttctggaaa taggcgtatt tcatatcgct ggagatattg attttgccga cgccgagcgt
    20161 aactgattca gcaatctccg cgtccgggtt tgccgaacca ccgtgtagca ccagcggaat
    20221 atcaatacgt ccggcaatgt cgcgcaaaat gtccatttgc agcttcggct gcatcccttt
    20281 tggatagatg ccatgcgctg taccaatcgc cacagccaga gtgtcggcac ctgtacgagc
    20341 aataaaatcg gcggcctgcg ccggatctgt gtaggtcact tgtgaaacac caccttctac
    20401 cgacgttcct gtttgtccga tggttcccag ttcaccctca accgacactc caaccgcgtg
    20461 agcgaggcga accacttctg cggtcagcgc cacgttttct tcatacggca gcagcgaacc
    20521 gtcgatcatt actgaggtaa atccgcagcg aatcgcacgc aaaacatgct ctacagaggc
    20581 accatgatcg aggtgcagta caaacgggac cggactacgc tgggtaattt cgcgaacgtg
    20641 agcgaaaaag gcatcgccca caaaatcatg ttcgctggga tggatggcaa taatcgccgg
    20701 agtatttgtc gcttctgctt catccacgac tgcgcgaagg aaacagttat ccgccacatt
    20761 aaatgcgcca atggcaaagc cgtgctggcg ggtaggttgc agtaattctt tcatcgaaac
    20821 taacatgtta aatctccagt ttcagcgtgt agtccgccct gccggtgcgt tattgcactg
    20881 gcataaaggc catgacagaa aatgaatcag gctttgccga tagagccgct cgcctgtaaa
    20941 tacggtgtaa tcgcttcttc gaaggccagt tccatagctt tcagcgcgtc caccagttca
    21001 atggatatat cgtgacagag ggtatgcagg agcgcggatt tcccccgttc aaaaatggcc
    21061 gcccccacgt tgatttttgc tacccccagc gtcgtacaga gctggagctg atcgaacggc
    21121 gtaccgctac cgccatgtaa tgccagaggc acgctcgagc agtggaaaat ctccgccaga
    21181 cgctgaaaat caatagcagg tacaacacca ggcgcgtaca tgccgtgcgc cgtgcctacc
    21241 gagacggcca gtagatcaat gcctgtctgc gcgataaacg tttccacatc agtgggttgc
    21301 gtcatcaggt ctgcattggc ggcctggtcg taacgcggcg catctgccag atggcccagt
    21361 tcgccttcca cgcagagctg cgcgctggcg gcgagttcca ccaccgccct ggtctggcgc
    21421 acgttttcct ccaggggaaa agcagaagca tcaatcatta atccgctgaa accagcacga
    21481 aacgcctgac gaatacagtc cggatttttg ccatgatcaa gcgccagagc gaccggtact
    21541 gaggcagatt ccgcgagcgt tttaaccact gccgccgcga cgggtgctgg aaaatgcgcc
    21601 agatgcccct gatagagatt gagaatgatc ggagcgcgat tcttctccgc cacgcggata
    21661 gctgcgtgag cactttccag attcatacag ttgatcgcca gtaaggcgta atgttcatgc
    21721 caggctttca tcaccatact tttcatatcg gcaaacatag ggcctccggt tattcgaggg
    21781 tgaaagtgat ctcttcatct ttcagctttt gatcgctcag ttcgtcaaac tcttcgtcgc
    21841 gttccgttac cggtttcttc cagagtgaaa gcatcacgcc acaaatcacc gtgcctatac
    21901 ccagcgccag gcaaaacagc agaggatggg tgaacagagg cacaacaaac atgccgccat
    21961 gcggaaccgg actctccacg ccccacacca tgattaagcc gcccgcgacc gccgatgcca
    22021 ccacacaaga tcccactact cgcagcaggt cacgcgcggc gatcggaatg actccttcag
    22081 tgatcatgca gatccccatc ggaaacgcgg ctttcagcgc ctcgcgctcc gcacgggtgt
    22141 atttatgccg tgtcagtaag aacgaaagtg tgatgccaaa cggagggatg attgagccga
    22201 caaatttcac ggcttctggt ccgtagacgc cactgaccag caagccatcg gaaaacagcg
    22261 acatggtttt gttgaccgga cca
//
LOCUS       DAMZFT010000080        21626 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.080, whole genome shotgun sequence.
ACCESSION   DAMZFT010000080 DAMZFT010000000
VERSION     DAMZFT010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21626)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21626)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21626
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.080"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(158..1000)
                     /gene="purU"
                     /locus_tag="PN977_003141"
     CDS             complement(158..1000)
                     /gene="purU"
                     /locus_tag="PN977_003141"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HDI6043151.1"
                     /translation="MHSLQRKVLRTICPDQKGLIARITNICYKHELNIVQNNEFVDHR
                     TGRFFMRTELEGIFNDSTLLADLDSALPEGSVRELNPAGRRRIVILVTKEAHCLGDLL
                     MKANYGGLDVEIAAVIGNHDTLRSLVERFDIPFELVSHEGLSRNEHDQKMADAIDAYQ
                     PDYVVLAKYMRVLTPEFVARFPNKIINIHHSFLPAFIGARPYHQAYERGVKIIGATAH
                     YVNDNLDEGPIIMQDVIHVDHTYTAEDMMRAGRDVEKNVLSRALYKVLAQRVFVYGNR
                     TIIL"
     gene            complement(1050..1508)
                     /locus_tag="PN977_003142"
     CDS             complement(1050..1508)
                     /locus_tag="PN977_003142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YchJ family protein"
                     /protein_id="HDI6043152.1"
                     /translation="MSQLCPCGSAVEYSLCCHPYVSGEKVAPDPEHLMRSRYCAFVMQ
                     DADYLIKTWHPSCGAAALRAELMAGFAHTEWLGLTVFEHCWQDGDNIGFVSFVARFTE
                     GGKTGAIIERSRFLKENGQWYYIDGTRPQFGRNDPCPCGSGKKFKKCCGQ"
     gene            1621..2526
                     /gene="rssA"
                     /locus_tag="PN977_003143"
     CDS             1621..2526
                     /gene="rssA"
                     /locus_tag="PN977_003143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase RssA"
                     /protein_id="HDI6043153.1"
                     /translation="MRKIKIGLALGSGAARGWSHIGVINALKKVGIEIDIVAGCSIGS
                     LVGAAYACDRLSALEDWVTSFSYWDVLRLMDLSWQRGGLLRGERVFNQYREIMPETEI
                     ENCSRRFAAVATNLSTGRELWFTEGDLHLAIRASCSIPGLMAPVAHNGYWLVDGAVVN
                     PIPISLTRALGADIVIAVDLQHDAHLMQQDLLSFNVSEENSENGDSLPWHARLKERLG
                     SITTRRAVTAPTATEIMTTSIQVLENRLKRNRMAGDPPDILIQPVCPQISTLDFHRAH
                     AAIAAGQLAVEKKMDELLPLVRTNI"
     gene            2618..3631
                     /gene="rssB"
                     /locus_tag="PN977_003144"
     CDS             2618..3631
                     /gene="rssB"
                     /locus_tag="PN977_003144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RssB"
                     /protein_id="HDI6043154.1"
                     /translation="MTQPLVGKQILIVEDEQVFRSLLDSWFSSLGATTVLAADGVDAL
                     ELLGGFTPDLMICDIAMPRMNGLKLLEHIRNRGDQTPVLVISATENMADIAKALRLGV
                     EDVLLKPVKDLNRLREMVFACLYPSMFNSRVEEEERLFRDWDAMVDNPAAAAKLLQEL
                     QPPVQQVISHCRVNYRQLVAADKPGLVLDIAALSENDLAFYCLDVTRAGHNGVLAALL
                     LRALFNGLLQEQLAHQNQRLPELGALLKQVNHLLRQANLPGQFPLLVGYYHRELKNLI
                     LVSAGLNATLNTGEHQVQISNGVPLGTLGNAYLNQLSQRCDAWQCQIWGTGGRLRLML
                     SAE"
     gene            3833..4741
                     /gene="galU"
                     /locus_tag="PN977_003145"
     CDS             3833..4741
                     /gene="galU"
                     /locus_tag="PN977_003145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HDI6043155.1"
                     /translation="MAAINTKVKKAVIPVAGLGTRMLPATKAIPKEMLPLVDKPLIQY
                     VVNECIAAGITEIVLVTHSSKNSIENHFDTSFELEAMLEKRVKRQLLDEVQSICPPHV
                     TIMQVRQGLAKGLGHAVLCAHPVVGDEPVAVILPDVILDEYESDLSQDNLAEMIRRFD
                     ETGHSQIMVEPVADVTAYGVVDCKGVELAPGESVPMVGVVEKPKADVAPSNLAIVGRY
                     VLSADIWPLLAKTPPGAGDEIQLTDAIDMLIEKETVEAYHMKGKSHDCGNKLGYMQAF
                     VEYGIRHNTLGSEFKAWLEEEMGIKK"
     gene            complement(4886..5299)
                     /gene="hns"
                     /locus_tag="PN977_003146"
     CDS             complement(4886..5299)
                     /gene="hns"
                     /locus_tag="PN977_003146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator H-NS"
                     /protein_id="HDI6043156.1"
                     /translation="MSEALKILNNIRTLRAQARECTLETLEEMLEKLEVVVNERREEE
                     SAAAAEVEERTRKLQQYREMLIADGIDPNELLNSLAAVKSGTKAKRAQRPAKYSYVDE
                     NGETKTWTGQGRTPAVIKKAMDEQGKSLDDFLIKQ"
     gene            5903..6520
                     /gene="tdk"
                     /locus_tag="PN977_003147"
     CDS             5903..6520
                     /gene="tdk"
                     /locus_tag="PN977_003147"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HDI6043157.1"
                     /translation="MAQLYFYYSAMNAGKSTALLQSSYNYQERGMRTVVYTAEIDDRF
                     GAGKVSSRIGLSSPAKLFNQNSSLFDEIRAEHEQQAIHCVLVDECQFLTRQQVYELSE
                     VVDQLDIPVLCYGLRTDFRGELFIGSQYLLAWSDKLVELKTICFCGRKASMVLRLDQA
                     GRPYNEGEQVVIGGNERYVSVCRKHYKEALEVGSLTAIQERHRHD"
     gene            complement(6823..9498)
                     /gene="adhE"
                     /locus_tag="PN977_003148"
                     /gene_synonym="adhC"
     CDS             complement(6823..9498)
                     /gene="adhE"
                     /locus_tag="PN977_003148"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HDI6043158.1"
                     /translation="MAVTNVAELNALVERVKKAQREYASFTQEQVDKIFRAAALAAAD
                     ARIPLAKMAVAESGMGIVEDKVIKNHFASEYIYNAYKDEKTCGVLSEDDTFGTITIAE
                     PIGIICGIVPTTNPTSTAIFKSLISLKTRNAIIFSPHPRAKDATNKAADIVLQAAIAA
                     GAPKDLIGWIDQPSVELSNALMHHPDINLILATGGPGMVKAAYSSGKPAIGVGAGNTP
                     VVIDETADIKRAVASVLMSKTFDNGVICASEQSVVVVDSVYDAVRERFATHGGYLLQG
                     KELKAVQDVILKNGALNAAIVGQPAYKIAELAGFSVPENTKILIGEVTVVDESEPFAH
                     EKLSPTLAMYRAKDFEDAVEKAEKLVAMGGIGHTSCLYTDQDNQPARVSYFGQKMKTA
                     RILINTPASQGGIGDLYNFKLAPSLTLGCGSWGGNSISENVGPKHLINKKTVAKRAEN
                     MLWHKLPKSIYFRRGSLPIALDEVITDGHKRALIVTDRFLFNNGYADQITSVLKAAGV
                     ETEVFFEVEADPTLSIVRKGAELANSFKPDVIIALGGGSPMDAAKIMWVMYEHPETHF
                     EELALRFMDIRKRIYKFPKMGVKAKMIAVTTTSGTGSEVTPFAVVTDDATGQKYPLAD
                     YALTPDMAIVDANLVMDMPKSLCAFGGLDAVTHAMEAYVSVLASEFSDGQALQALKLL
                     KEYLPASYHEGSKNPVARERVHSAATIAGIAFANAFLGVCHSMAHKLGSQFHIPHGLA
                     NALLICNVIRYNANDNPTKQTAFSQYDRPQARRRYAEIADHLGLSAPGDRTAAKIEKL
                     LAWLETLKAELGIPKSIREAGVQEADFLANVDKLSEDAFDDQCTGANPRYPLISELKQ
                     ILLDTYYGRDYVEGETAAKKEAAPAKAEKKAKKSA"
     gene            9975..10622
                     /gene="ychE"
                     /locus_tag="PN977_003149"
     CDS             9975..10622
                     /gene="ychE"
                     /locus_tag="PN977_003149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YchE"
                     /protein_id="HDI6043159.1"
                     /translation="MIQTLFDFPVYFKFFIGLFALVNPVGIIPVFISMTSYQTAAARN
                     KTNLTANLSVAIILWISLFLGDTILQLFGISIDSFRIAGGILVVTIAMSMISGKLGED
                     KQNKQEKSETAVRESIGVVPLALPLMAGPGAISSTIVWGTRYHSISYLFGFFVAIALF
                     ALCCWGLFRMAPWLVRVLRQTGINVITRIMGLLLMALGIEFIVTGIKGIFPGLLN"
     gene            complement(10780..10941)
                     /locus_tag="PN977_003150"
     CDS             complement(10780..10941)
                     /locus_tag="PN977_003150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6043160.1"
                     /translation="MSIFSDYSSSSEMHNNLTIDYYLALSSTKGSGITNIISIILQQA
                     QDYDVAKIT"
     gene            11360..12991
                     /gene="oppA"
                     /locus_tag="PN977_003151"
     CDS             11360..12991
                     /gene="oppA"
                     /locus_tag="PN977_003151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein OppA"
                     /protein_id="HDI6043161.1"
                     /translation="MTNITKRSLVAAGVLAALMAGNVALAADVPAGVTLAEKQTLVRN
                     NGSEVQSLDPHKIEGVPESNISRDLFEGLLVSDLDGHPAPGVAESWDNKDAKVWTFHL
                     RKDAKWSDGTPVTAQDFVYSWQRSVDPNTASPYASYLQYGHIAGIDEILEGKKPITDL
                     GVKAIDDHTLEVTLSEPVPYFYKLLVHPSTSPVPKAAIEKFGEKWTQPGNIVTNGAYT
                     LKDWVVNERIVLERSPTYWNNAKTVINQVTYLPIASEVTDVNRYRSGEIDMTYNNMPI
                     ELFQKLKKEIPDEVHVDPYLCTYYYEINNQKPPFNDVRVRTALKLGMDRDIIVNKVKA
                     QGDMPAYGYTPPYTDGAKLTQPEWFGWSQEKRNEEAKKLLAEAGYTADKPLTINLLYN
                     TSDLHKKLAIAASSLWKKNIGVNVKLVNQEWKTFLDTRHQGTFDVARAGWCADYNEPT
                     SFLNTMLSNSSMNTAHYKSPAFDSIMAETLKATDEAQRTALYTKAEQQLDKDSAIVPV
                     YYYVNARLVKPWVGGYTGKDPLDNTYTRNMYIVKH"
     gene            13077..13997
                     /gene="oppB"
                     /locus_tag="PN977_003152"
     CDS             13077..13997
                     /gene="oppB"
                     /locus_tag="PN977_003152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppB"
                     /protein_id="HDI6043162.1"
                     /translation="MLKFILRRCLEAIPTLFILITISFFMMRLAPGSPFTGERTLPPE
                     VMANIEAKYHLNDPIMTQYFSYLKQLAHGDFGPSFKYKDYSVNDLVASSFPVSAKLGA
                     AAFFLAVILGVSAGVIAALKQNTKWDYTVMGLAMTGVVIPSFVVAPLLVMIFAIILHW
                     LPGGGWNGGALKFMILPMVALSLAYIASIARITRGSMIEVLHSNFIRTARAKGLPMRR
                     IILRHALKPALLPVLSYMGPAFVGIITGSMVIETIYGLPGIGQLFVNGALNRDYSLVL
                     SLTILVGALTILFNAIVDVLYAVIDPKIRY"
     gene            14012..14920
                     /gene="oppC"
                     /locus_tag="PN977_003153"
     CDS             14012..14920
                     /gene="oppC"
                     /locus_tag="PN977_003153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000979653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppC"
                     /protein_id="HDI6043163.1"
                     /translation="MMLSKKNSETLENFSEKLEVEGRSLWQDARRRFMHNRAAVASLI
                     VLVLIALFVILAPMLSQFAYDDTDWAMMSSAPDMESGHYFGTDSSGRDLLVRVAIGGR
                     ISLMVGVAAALVAVVVGTLYGSLSGYLGGKVDSVMMRLLEILNSFPFMFFVILLVTFF
                     GQNILLIFVAIGMVSWLDMARIVRGQTLSLKRKEFIEAAQVGGVSTPGIVIRHIVPNV
                     LGVVVVYASLLVPSMILFESFLSFLGLGTQEPLSSWGALLSDGANSMEVSPWLLLFPA
                     GFLVVTLFCFNFIGDGLRDALDPKDR"
     gene            14932..15945
                     /gene="oppD"
                     /locus_tag="PN977_003154"
     CDS             14932..15945
                     /gene="oppD"
                     /locus_tag="PN977_003154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP binding protein OppD"
                     /protein_id="HDI6043164.1"
                     /translation="MSVIETATVPLAQQQADALLNVKDLRVTFNTPDGDVTAVNDLNF
                     SLRAGETLGIVGESGSGKSQTAFALMGLLAANGRIGGSATFNGREILNLPERELNKLR
                     AEQISMIFQDPMTSLNPYMRVGEQLMEVLMLHKNMSKAEAFEESVRMLDAVKMPEARK
                     RMKMYPHEFSGGMRQRVMIAMALLCRPKLLIADEPTSALDVTVQAQIMTLLNELKREF
                     NTAIIMITHDLGVVAGICDKVLVMYAGRTMEYGNARDVFYQPVHPYSIGLLNAVPRLD
                     AEGETMLTIPGNPPNLLRLPKGCPFQPRCPHAMEICSSAPPLEEFTPGRLRACFKPVE
                     ELL"
     gene            15942..16946
                     /gene="oppF"
                     /locus_tag="PN977_003155"
     CDS             15942..16946
                     /gene="oppF"
                     /locus_tag="PN977_003155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP binding protein OppF"
                     /protein_id="HDI6043165.1"
                     /translation="MNAVTEGRKVLLEIADLKVHFEIKDGKQWFWQPPKTLKAVDGVT
                     LRLYEGETLGVVGESGCGKSTFARAIIGLVKATDGHVAWLGKELLGMKPDEWRAVRSD
                     IQMIFQDPLASLNPRMTIGEIIAEPLRTYHPKMSRQEVRERVKAMMLKVGLLPNLINR
                     YPHEFSGGQCQRIGIARALILEPKLIICDEPVSALDVSIQAQVVNLLQQLQREMGLSL
                     IFIAHDLAVVKHISDRVLVMYLGHAVELGTYDEVYHNPLHPYTRALMSAVPIPDPDLE
                     KNKTIQLLEGELPSPINPPSGCVFRTRCPIAGPECAKTRPVLEGSFRHAVSCLKVDPL
                     "
     gene            complement(16999..17328)
                     /locus_tag="PN977_003156"
     CDS             complement(16999..17328)
                     /locus_tag="PN977_003156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciU family protein"
                     /protein_id="HDI6043166.1"
                     /translation="MDMDLNNRLTEDETLEQAYDIFLELAADNLDPADVLLFNLQFEE
                     RGGAELFDPAEDWQEHVDFDLNPDFFAEVVIGLADSEDGEINDVFARILLCREKDHKL
                     CHIIWRE"
     gene            complement(17363..18823)
                     /gene="cls"
                     /locus_tag="PN977_003157"
     CDS             complement(17363..18823)
                     /gene="cls"
                     /locus_tag="PN977_003157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HDI6043167.1"
                     /translation="MTTVYTLVSWLAILGYWLLIAGVTLRILMKRRAVPSAMAWLLII
                     YILPLVGIIAYLAVGELHLGKRRAERARAMWPSTAKWLNDLKACKHIFAEENSSVAAP
                     LFKLCERRQGIAGVKGNQLQLMTESDDVMQALIRDIQLARHNIEMVFYIWQPGGMADQ
                     VAESLMAAARRGIHCRLMLDSAGSVAFFRSPWPELMRNAGIEVVEALKVNLMRVFLRR
                     MDLRQHRKMIMIDNYIAYTGSMNMVDPRYFKQDAGVGQWIDLMARMEGPIATAMGIIY
                     SCDWEIETGKRILPPPPDVNIMPFEQASGHTIHTIASGPGFPEDLIHQALLTAAYSAR
                     EYLIMTTPYFVPSDDLLHAICTAAQRGVDVSIILPRKNDSMLVGWASRAFFTELLAAG
                     VKIYQFEGGLLHTKSVLVDGELSLVGTVNLDMRSLWLNFEITLAIDDKGFGADLAAVQ
                     DDYISRSRLLDARLWLKRPLWQRVAERLFYFFSPLL"
     gene            18966..19139
                     /locus_tag="PN977_003158"
     CDS             18966..19139
                     /locus_tag="PN977_003158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciY family protein"
                     /protein_id="HDI6043168.1"
                     /translation="MKRSRTEVGRWRMQRQASRRKSRWLEGQSRRNMRIHSIRKCILN
                     KQRNSLLFAIYNI"
     gene            complement(19194..20447)
                     /gene="kch"
                     /locus_tag="PN977_003159"
     CDS             complement(19194..20447)
                     /gene="kch"
                     /locus_tag="PN977_003159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated potassium channel protein"
                     /protein_id="HDI6043169.1"
                     /translation="MSHWATFKQTATNLWVTLRHDILALAVFLNGLLIFKTIYGMSVN
                     LLDIFHIKAFSELDLSLLANAPLFMLGVFLVLNSIGLLFRAKLAWAISIILLLIALIY
                     TLHFYPWLKFSIGFCIFTLVFLLILRKDFSHSSAAAGTIFAFISFTTLLFYSTYGALY
                     LSEGFNPRIESLMTAFYFSIETMSTVGYGDIVPVSESARLFTISVIISGITVFATSMT
                     SIFGPLIRGGFNKLVKGNNHTMHRKDHFIVCGHSILAINTILQLNQRGQNVTVISNLP
                     EDDIKQLEQRLGDNADVIPGDSNDSSVLKKAGIDRCRAILALSDNDADNAFVVLSAKD
                     MSSDVKTVLAVSDSKNLNKIKMVHPDIILSPQLFGSEILARVLNGEEINNDMLVSMLL
                     NSGHGIFSDNDEQETKADNKESAQK"
     gene            complement(21088..>21626)
                     /locus_tag="PN977_003160"
     CDS             complement(21088..>21626)
                     /locus_tag="PN977_003160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HDI6043170.1"
                     /translation="ERQFAVPEPDRVWCGDITYIWAGNRWCYLAVVMDLFARRVIGWS
                     LSANADTALISSALRMAYEVRGQPRDVMFHSDQGSQYTGLKYQQLLWRYRIKQSVSRR
                     GNCWDNSPMERFFRSLKTEWVPTDGYVGKDEARQQISGYILNYYNSVRPHHYNGGLTP
                     EESENRYRFYCKTVANIT"
BASE COUNT         5609 a         4955 c         5305 g         5757 t
ORIGIN      
        1 ggggtaatgc tttttactgg cctgctccct tatcgggaag cggggcgcat catatcaaat
       61 gacgcgccgc tgtaaagtgt tacgttgaga aaaatgaact ggttgcgtaa ttttcatccg
      121 taacggatta aaggtaacca gttatttttg ctggcgatta aagaataatc gtccgattac
      181 cgtaaacaaa gacgcgctgc gccagtactt tgtagagtgc gcgacttaag acgtttttct
      241 cgacgtcacg acctgcgcgc atcatatctt cagctgtgta ggtatgatcg acatgaataa
      301 cgtcctgcat gatgattgga ccttcgtcca gattgtcatt cacatagtga gcggttgcgc
      361 caataatctt cacgccacgt tcataggcct ggtgataagg acgtgcgccg ataaacgctg
      421 gcaggaatga atggtgaata ttgatgatct tattcgggaa gcgtgccaca aattctggcg
      481 ttaatacccg catatacttc gccagcacca cgtagtcagg ctgataagca tcaatggcat
      541 ccgccatctt ttgatcgtgc tcgttgcgac ttaatccttc atgacttacc agctcaaacg
      601 gaatatcaaa acgctcaacc agagaacgta aagtatcgtg gttaccaata actgccgcga
      661 tttcgacatc caggccgccg taattggctt tcatcaacaa atcgccaagg caatgcgcct
      721 ctttagtgac cagaatcact atccggcgac gaccggcagg attcagctca cgtacggagc
      781 cttctggcaa tgcgctatcg agatccgcca gcagggtgga atcattaaaa atcccctcca
      841 gttccgtgcg cataaaaaag cgcccggtac ggtgatcaac aaattcattg ttctgtacga
      901 tatttaactc gtgcttgtag caaatattgg tgatacgtgc gatcagacct ttttggtccg
      961 gacaaatagt acgcagaact ttacgttgga gtgaatgcat tgctggaaaa accttgttga
     1021 gagtgtttgc taaaccgtac cgtcaaccat tattggccgc agcacttttt aaatttttta
     1081 cctgaaccac aagggcaggg atcgttgcgg ccaaactgcg gacgtgtacc gtcaatatag
     1141 taccattgac cgttttcttt caaaaatcgc gaacgttcaa tgattgcacc tgttttgcca
     1201 ccttcggtaa atcgagcaac aaaactgaca aatccgatat tgtcgccgtc ctgccaacaa
     1261 tgttcaaata cggtcaatcc caaccattca gtatgcgcaa atccggccat caattcggca
     1321 cgtaacgccg ctgctccaca agatggatgc caggtcttta ttaaataatc tgcgtcttgc
     1381 atcacaaaag cgcagtaacg cgaacgcatg agatgttctg gatcaggtgc aaccttttca
     1441 ccagacacat aagggtggca acataggcta tactcgacag cactaccaca gggacaaagc
     1501 tgagacacaa ataatctccc tggaaacaat aacggcgtat taaccgcctg agtagcacta
     1561 tgttaaccga gcagtagcga tgtggctacg attgcattcc aggggaatct tgcgggaata
     1621 atgagaaaga taaaaatagg gctggcgctg ggatctggcg cggcgagagg ttggtcgcat
     1681 attggcgtta ttaatgcgct aaaaaaagtg ggtattgaaa ttgatatcgt tgcaggatgc
     1741 tcaattggtt cgctggtggg cgcggcctat gcatgcgatc gattatctgc gctggaagat
     1801 tgggtaacct ctttcagtta ttgggatgtt ttacgcctga tggatctctc ctggcagcgc
     1861 ggtgggttac tgcgcggcga gcgtgtcttc aatcaatatc gcgaaataat gccggaaaca
     1921 gagatcgaaa attgttcccg tcgcttcgcg gctgttgcca ccaatttaag tactgggcgc
     1981 gaattatggt ttactgaagg cgatctccat cttgctattc gtgcatcatg cagtattcca
     2041 ggactaatgg cacccgttgc gcataacggc tactggctgg ttgatggcgc tgtagttaac
     2101 ccaattccta tttcgctcac gcgtgcattg ggtgctgata ttgtgatagc ggtcgacctg
     2161 cagcacgatg ctcatttgat gcaacaagat ctgctctcct ttaatgtcag tgaagaaaat
     2221 agcgagaatg gtgattctct gccgtggcat gcgcgtctga aagaaaggtt gggcagcata
     2281 acgacacgtc gggcggtgac agcgccaacg gcaacagaga ttatgaccac ttctatccag
     2341 gtgctggaga accgccttaa aaggaaccgc atggcaggtg atccgcccga tattctgatt
     2401 caacctgttt gcccgcaaat atctacgctt gatttccatc gcgcgcacgc tgccattgcg
     2461 gctgggcagc tggcagtgga aaagaaaatg gacgaacttt tgccgttggt acgcaccaac
     2521 atttgaccag aatttttatc tacacttaag ttaattctga caggcgcagg tggcaatagc
     2581 atgccactat tgagtaaagc cagtcagggg agagaacatg acgcagccat tggtcggaaa
     2641 acagattctc attgttgaag atgagcaggt atttcgctcg cttctggatt cgtggttttc
     2701 ctcattggga gcgacaacgg tactggcggc tgatggggtg gatgcccttg agttgctggg
     2761 aggtttcact ccagacctga tgatatgtga tatcgcgatg cccagaatga acgggcttaa
     2821 actgctggaa catatacgca acagaggtga ccagacaccg gttctggtga tatctgccac
     2881 tgaaaatatg gcagatattg ccaaagcgtt acgtctgggc gttgaagatg ttttgctgaa
     2941 accagttaaa gatctgaatc gcttgcgcga gatggttttt gcctgtctct atcccagcat
     3001 gtttaattcc cgcgttgagg aagaggaaag gctttttcgc gactgggatg caatggttga
     3061 taaccctgcc gcagcggcga aattattaca ggaattgcaa ccgccggttc agcaggtgat
     3121 ttcccattgc cgggttaatt atcgtcaatt ggttgccgcg gacaaacccg gcctggtgct
     3181 tgatattgcc gcactttcgg aaaacgatct ggcattttat tgccttgatg tcacccgagc
     3241 tggacataat ggcgtacttg ctgccttgtt attacgcgca ttgtttaacg gattattaca
     3301 ggaacagctt gcacaccaaa atcaacggtt gccagagttg ggcgcgttat tgaagcaggt
     3361 aaaccattta ctccgtcagg ccaatctgcc ggggcagttt ccgctattag ttggctatta
     3421 tcatcgcgaa ctgaaaaatc tcattctggt ttctgcgggt ctgaatgcga cgttaaatac
     3481 cggcgaacac caggtacaaa tcagtaatgg tgttccgtta ggcactttag gtaacgctta
     3541 tttgaatcaa ttgagccagc gatgcgatgc ctggcaatgc caaatatggg gaaccggtgg
     3601 tcggctgcgc ttgatgttgt ctgcagaatg agcaaacgat aacgcgggct aaatttgcat
     3661 tacctgctaa tgtcggctgg tggtactatc gtcgccattc gtataagtaa ttgtcttaat
     3721 tatgctaact cgcctccttt tcagaactta gccccttcgg ggtactgata tactgggatg
     3781 cgatacagaa atatgaacac gttcaaaaca cgaacagtcc aggagaattt aaatggctgc
     3841 cattaatacg aaagtcaaaa aagccgttat ccccgttgcg ggattaggaa ccaggatgtt
     3901 gccggcgacg aaagccatcc cgaaagagat gctgccactt gtcgataagc cattaattca
     3961 atacgtcgtg aatgaatgta ttgcggctgg cattactgaa attgtgctgg ttactcactc
     4021 atctaaaaac tctattgaaa accactttga taccagtttt gaactggaag caatgctgga
     4081 aaaacgtgta aaacgtcaac tgcttgatga agtgcagtct atttgtccgc cgcacgtgac
     4141 tattatgcaa gttcgtcagg ggctggcgaa aggcctggga cacgcggtat tgtgtgctca
     4201 cccggtagtg ggtgatgaac ctgtggctgt tattttgcct gacgttattc tggatgaata
     4261 tgaatccgat ttgtcacagg ataacctggc agagatgatc cgccgctttg atgaaacggg
     4321 tcatagccag atcatggttg aaccggttgc tgatgtgacc gcatatggcg ttgtggattg
     4381 caaaggcgtt gaattagcgc cgggtgaaag cgtaccgatg gttggcgtgg ttgaaaaacc
     4441 aaaagcggat gttgcgccgt ctaatctcgc tattgtgggt cgttacgttc ttagcgcgga
     4501 tatttggccg ttgctggcaa aaacccctcc gggtgctggt gatgaaattc agctcaccga
     4561 cgcaattgat atgctgatcg aaaaagaaac ggtggaagcc tatcatatga aagggaagag
     4621 ccatgactgc ggtaataaat taggttacat gcaggccttc gttgaatacg gtattcgtca
     4681 taacaccctt ggctcggaat ttaaagcctg gcttgaagaa gagatgggca ttaagaagta
     4741 acatccgtat cggtgttatc cacgaaacgg cgttgagcaa tcgacgccgt ttttttatag
     4801 cttattctta ttaaattgtc ttaaaccgga caataaaaaa tcccgccgat ggcgggattt
     4861 ttaagcaagt gcaatctaca aaagattatt gcttgatcag gaaatcgtcg agggatttac
     4921 cttgctcatc cattgctttt ttgattacag ctggagtacg gccctggcca gtccaggttt
     4981 tagtttcgcc gttttcgtca acgtagctat attttgccgg acgctgagca cgtttagctt
     5041 tggtgccaga tttaacggca gcaaggctat tcagcagttc gttcgggtca ataccgtcag
     5101 cgatcagcat ttcgcgatat tgctgcagtt tacgagtgcg ctcttcaact tcagcagcag
     5161 ccgcgctttc ttcttcgcga cgttcgttaa caacaacttc taatttttcc agcatttctt
     5221 ccagcgtttc aagtgtacat tctcttgcct gcgcacgaag agtacggatg ttgttcagaa
     5281 ttttaagtgc ttcgctcatt gtagtaatct caaacttata ttggggtggt ttgttgaggt
     5341 aataatagag ccttaaattc agttgtgcaa tagccaggaa tgtaaggaat tcaaaattgt
     5401 tctttatttt gtgccgccaa taaatatctt ttcataaaat tagccagaaa agacgcggca
     5461 tatagcccta tttgcaccga tgatttcgca gcacgtgagg ttaaaacttc ctgattcatg
     5521 tcacatttta tggggagatt atcgtaggct gacgaccttt cagtcttctg tattagtcgt
     5581 gtttacgaga attccctatt aagcgaatga tgaaaagtag aacagtcgca ataagagcag
     5641 ggacttagta ttgcactatc tccaggaggt caacagaggg ctattacttg cgcaacaggt
     5701 taaagattgt gaatagttac cagcagtcat ttacccgctt ataacaagcg aggcagttgt
     5761 aatgatagct cagaaggatt atgcaaggct tcgtaaggga gaacgcatat accacttctg
     5821 tgcatactgt tgagctgaaa aactgacgaa ttatgataaa ctccagccaa ctttaattca
     5881 tatcattgag ggcctgtggc tgatggcaca gctatatttc tactattccg caatgaatgc
     5941 gggtaagtct acagcattgt tgcaatcttc atacaattac caggagcgcg gcatgcgcac
     6001 tgtcgtatat acggcagaaa ttgatgatcg ctttggtgcc gggaaagtca gctcgcgtat
     6061 aggtttgtca tcgcctgcaa aattatttaa ccaaaattca tcattatttg atgagattcg
     6121 tgcggaacat gaacagcagg caattcattg cgtactggtt gatgaatgcc agtttttaac
     6181 cagacaacaa gtatatgaat tatcggaggt tgtcgatcaa ctcgatatac ccgtactttg
     6241 ttatggttta cgtaccgatt ttcgaggtga gttatttatt ggcagccaat acttactggc
     6301 atggtccgac aaactggttg aattaaaaac catctgtttt tgtggccgca aagcaagcat
     6361 ggtgctgcgt cttgatcaag caggcagacc ttataacgaa ggtgagcagg tggttattgg
     6421 tggtaatgaa cgctacgttt ctgtatgccg taaacactat aaagaagcgt tagaagtcgg
     6481 ctcattaacg gctattcagg aaaggcatcg ccacgattaa taagaatttc tttactgaca
     6541 gggtgagcag ggcgctttta tcctgtcagt tcattttacg cacttcatcc ggtgtatata
     6601 cccttctcga cagttcttta acgccactat acccctcaca gatcgctttt acagtgacgt
     6661 tgacttgtga cctgtaaagt acaatatcgc tgtgtgtagg cgttatacat cgccgtaaat
     6721 atgatgaagg caaatgctgt cggtttatgg gaaagttgct ttgggtaaaa caaaaaaacg
     6781 gccccagaag ggaccgttta tgttgccaga cagcgctact gattaagcgg atttcttcgc
     6841 ttttttctca gctttagccg gagcggcttc ttttttcgct gcagtttcac cttctacata
     6901 atcacgaccg tagtaggtat ccagcagaat ctgtttcagc tcggagatca gtgggtaacg
     6961 cgggttagcg ccggtgcact ggtcatcgaa tgcatcttca gacagtttat ccacattcgc
     7021 caggaagtct gcttcctgaa cgccagcttc acggatagat ttcggaatac ccagttcagc
     7081 tttcagcgtt tccagccatg ccagcagttt ctcgatctta gcagcagtac ggtcgcccgg
     7141 tgcgctcaga cccaggtggt cagcaatttc agcataacga cggcgagcct gcggacggtc
     7201 atactggctg aatgcagtct gcttggtcgg gttgtcgttc gcgttgtagc gaataacgtt
     7261 acaaatcagc agggcgtttg ccagaccgtg cggaatatgg aactgggaac ccagtttgtg
     7321 cgccattgag tgacatacac ccaggaaggc gttcgcaaac gcgatacccg cgatagttgc
     7381 tgcactgtga acacgttcac gcgctaccgg atttttagaa ccttcgtggt aggacgctgg
     7441 cagatattct ttcagcagtt tcagtgcctg cagtgcctga ccatcagaga actcagatgc
     7501 cagtacagaa acataagctt ccatggcgtg agttactgcg tccagaccac cgaaagcaca
     7561 cagagacttc ggcatgtcca taaccaggtt ggcatcgaca atcgccatat ccggggtcag
     7621 cgcatagtct gccagcggat atttctgacc agtagcgtcg tcagttacaa ccgcaaacgg
     7681 agtgacttca gaacctgtac cagaagtggt ggtgacagcg atcattttcg ctttcacgcc
     7741 cattttcggg aacttgtaga tacgtttacg gatatccata aagcgcagcg ccagctcttc
     7801 gaagtgagtt tccggatgtt cgtacataac ccacatgatc ttcgcggcgt ccatcgggga
     7861 accaccacct agcgcgataa tcacgtctgg tttgaaggag tttgccagtt ctgcaccttt
     7921 acgaacgatg ctcagggtcg ggtcagcttc tacttcgaag aagacttcag tttcaacgcc
     7981 tgctgctttc agtacggaag tgatctgatc agcataacca ttgttgaaca ggaagcggtc
     8041 agtcacgatg agcgcacgtt tgtggccatc agtaatcact tcatccagcg cgattggcag
     8101 ggagccacgg cggaagtaga tagatttcgg aagtttgtgc cacaacatgt tttcagctcg
     8161 cttagcaacg gttttcttgt tgatcaggtg tttcggacca acgttttcag agatggagtt
     8221 accaccccag gaaccacaac ccagagtcag ggaaggtgcg agtttgaagt tatacaggtc
     8281 accgatacca ccctgagaag ccggggtgtt aatcaggata cgagccgttt tcattttctg
     8341 accgaagtaa gaaacgcgag ccggttggtt atcctggtca gtgtacaggc aagaggtatg
     8401 accgataccg cccatagcaa ccagtttctc tgctttttct accgcatctt cgaaatcttt
     8461 agcacggtac attgccagag tcggggacag tttttcatgt gcgaacggtt cgctttcatc
     8521 aacaacggtc acttcaccga tcagaatctt ggtgttttct ggtacagaga agcctgccag
     8581 ttcagcaatt ttataggctg gctgaccaac gatagccgcg ttcagcgcac cgtttttcag
     8641 gataacatcc tgaacagctt tcagctcttt accctgcaac agatagccgc cgtgggttgc
     8701 gaaacgttca cgtactgcgt cataaacaga gtcaacaaca acaacagact gttcagaagc
     8761 acagattacg ccgttgtcga aggttttgga catcagcaca gatgcaactg cacgtttgat
     8821 atcagcagtt tcatcgataa caacaggcgt gttacccgcg cctacaccga tagctggttt
     8881 accggagctg tatgcggctt taaccatgcc cggaccacca gtcgcgagga tcaggttgat
     8941 gtctgggtgg tgcatcagtg cgttagacag ttcaaccgaa ggttgatcga tccagccgat
     9001 cagatctttc ggagcaccgg cagcgatagc agcctgcaga acgatatcag ctgctttgtt
     9061 ggttgcatct tttgcacgcg ggtgcgggga gaagataata gcgttacggg tcttcaggct
     9121 gatcagcgat ttgaagatag cagttgaagt cgggttagtg gtcggaacga taccgcaaat
     9181 aataccgatt ggttcagcga tagtgatggt accaaaagtg tcgtcttcag acagaacacc
     9241 acaggttttt tcatctttat aggcgttgta gatatattca gaagcaaagt ggtttttgat
     9301 cactttatct tcgacgatac ccatgccgga ttcggcaacg gccattttcg cgagtgggat
     9361 tcgagcatct gcagcagcca gagcggcggc gcggaagatt ttgtctactt gctcttgagt
     9421 gaaactggca tattcacgct gggctttttt tacacgctct acgagtgcgt taagttcagc
     9481 gacattagta acagccataa tgctctcctg ataatgttaa acttttttag taaatcatct
     9541 gctcgaatac gagagtatag tcagtgcggt gatgatttgc ttaaccgatg gaaatcaaaa
     9601 gcttactcgc gctcacactc actgtgattt actaaaagag tttaaacatt agagttatta
     9661 tctctaatgc gtcacttcca ggtggcgtaa gcaagattac tcacttctgg gtactgatta
     9721 cgtgatctaa atcaaaattt tgcaaagctg acacctttca gcatggcttt tcgccattat
     9781 agctaacagt taataaattg tagtatgatt tgttgactac attagcatgt tttgcacaaa
     9841 tagataacaa taacgaatga tagcaatttt aagtagttag gaggtgaaaa atgctgtcaa
     9901 aaggcgtatt gtcagcgcgt cttttcaacc ttatttatgg ctaacattat ccggcttttg
     9961 cttcggagct aaccgtgatt cagacccttt ttgattttcc cgtttacttc aaatttttca
    10021 tcgggttatt tgcgctggtc aacccggtag ggattattcc cgtctttatc agcatgacca
    10081 gttatcagac agcggcagcg cgaaataaaa ctaaccttac agccaacctg tctgtggcca
    10141 ttatcttgtg gatctcgctt tttctcggcg acacgattct acaacttttt ggtatatcaa
    10201 ttgattcatt ccgtatcgcc ggtggcatcc tggtggtgac aatagcgatg tcgatgatca
    10261 gcggcaagct tggcgaggat aaacagaaca agcaagaaaa atcagaaacc gcggtacgtg
    10321 aaagcattgg tgtggtgccg ttggcgttgc cgttgatggc ggggccaggg gcgatcagtt
    10381 ctaccatcgt ctggggtacg cgttatcaca gcattagcta tctgtttggt ttctttgtgg
    10441 ctattgcttt gttcgcttta tgttgctggg gattgttccg catggcaccg tggctggtac
    10501 gggttttacg ccagaccggc atcaacgtga ttacgcgtat tatggggcta ttgctgatgg
    10561 cattggggat tgaatttatc gttactggta ttaaggggat tttccccggc ttgcttaatt
    10621 aattcctttc aaatgaaacg gagctgccat gctccgttta cttcgtcatt atttttactt
    10681 tgttccggtg cagttatcaa aagcaaaagg aatgggtaaa aatattcttc ttaaattaca
    10741 gtttgttata aggatttcct taactgcttc tcgtcaccat catgttattt tcgccacatc
    10801 ataatcctgg gcttgctgaa gaataattga aatgatatta gtaattccac tgcctttggt
    10861 agaggaaagt gctaaataat aatcaattgt taaattattg tgcatttcac tactggaact
    10921 gtaatcagaa aagatagaca tgcttaaccc atctctattt gattgaattg aaagatgttt
    10981 gttaagacat ggatgcaagc tatagattct gatacggtca ataaaagaga attgcttaac
    11041 aattttgcaa aatgtattgg cgagtaagaa ccgcatttgg tactttccgg gcaaccgcca
    11101 ggcgattctt tattggtaat gagaataatt aacaattaaa gagcgtcgtg aaagaataat
    11161 gtgtctcgac aggggagaca cagtacgaat cgacataagg tgatcgtctg aatcaccaga
    11221 ataaataaag tcggtgatag caatccgtaa cgataaagta acctgacagc agaaagtctc
    11281 cgagcctgtg cagggtccca atccgggatt acacatgctg gttaatacca gtaattataa
    11341 tgagggagtc caaaaaacaa tgaccaacat caccaagaga agtttagtag cagctggcgt
    11401 tttggctgcg cttatggcag ggaatgtcgc gctggcagct gatgtacccg caggtgtcac
    11461 actggcggaa aaacaaacgc tggtacgtaa caatggttca gaagttcagt cattagatcc
    11521 gcacaaaatt gaaggtgttc cggagtctaa tatcagccga gacctgtttg aaggcttact
    11581 ggttagcgat cttgacggcc atccggcacc tggcgtcgct gaatcctggg ataataaaga
    11641 cgcgaaagtc tggaccttcc atttgcgtaa agatgcgaaa tggtctgatg gcacgccagt
    11701 cacagcacaa gactttgtgt atagctggca acgttctgtt gatccaaaca ctgcttctcc
    11761 gtatgccagt tatctgcaat atgggcatat cgccggtatt gatgaaattc ttgaagggaa
    11821 aaaaccgatt accgatctcg gcgtgaaagc tattgatgat cacacattag aagtcacctt
    11881 aagtgaacct gttccgtact tctataaatt acttgttcac ccatcaactt caccggtgcc
    11941 aaaagccgct atcgagaaat tcggcgaaaa atggacccag cctggtaata tcgtcaccaa
    12001 cggtgcctat accttaaaag attgggtcgt aaacgaacga atcgttcttg aacgcagccc
    12061 gacctactgg aacaacgcga aaaccgttat taaccaagta acctatttgc ctattgcttc
    12121 tgaagttacc gatgtcaacc gctaccgtag tggtgaaatc gacatgactt ataacaacat
    12181 gccgatcgaa ttgttccaga agctgaaaaa agagatcccg gacgaagttc acgttgatcc
    12241 atacctgtgc acttactatt acgaaattaa caaccagaaa ccgccattta atgacgtgcg
    12301 tgtgcgtacc gcactgaaat tgggtatgga ccgcgatatc attgttaata aagtgaaagc
    12361 gcagggcgac atgcccgcct atggttacac tccaccatat actgatggcg caaaattgac
    12421 tcagccggaa tggtttggct ggagtcagga aaaacgtaac gaagaagcga aaaaactgct
    12481 ggctgaagcg ggttataccg cagacaaacc gttgaccatc aacctgttgt ataacacctc
    12541 cgatctgcat aaaaagctgg cgattgctgc ctcttcattg tggaagaaaa acattggtgt
    12601 aaacgtcaaa ctggttaacc aggagtggaa aacgttcctc gacacccgtc accagggcac
    12661 ttttgatgtg gcccgtgcag gctggtgtgc agactacaac gaaccaactt ccttcctgaa
    12721 caccatgctg tcgaacagct cgatgaatac cgcgcattat aagagcccgg cctttgacag
    12781 cattatggcg gaaacgctga aagcgactga cgaggcgcag cgcacagctc tgtacactaa
    12841 agcagaacaa cagctggata aggattcggc cattgttcct gtttattact acgtgaatgc
    12901 gcgtctggtg aaaccgtggg ttggtggcta taccggcaaa gatccgctgg ataataccta
    12961 cacccggaat atgtacattg tgaagcacta atggcaatac gtggggcagg agtgtcctgc
    13021 tccacggtgt ctgattttta tcgcattaca gaaggcacag gccagaaggt agggcaatgt
    13081 taaaatttat tctacgtcgc tgtctggaag cgattccgac gctatttatt cttattacta
    13141 tttcgttctt tatgatgcgc ctcgcaccgg gcagtccttt taccggcgaa cgtactttac
    13201 caccggaagt gatggccaat atcgaagcga aatatcatct taatgatccg atcatgacac
    13261 agtatttcag ctacctgaaa caactggcgc atggcgattt cggtccatcg tttaaatata
    13321 aagattattc ggtcaacgac ctggttgcat ccagttttcc cgtttctgcc aaactgggag
    13381 ccgcagcatt tttccttgcg gtaatactgg gtgttagtgc tggcgttatt gccgcattaa
    13441 aacaaaacac caaatgggac tataccgtga tggggctggc aatgaccggg gttgttatcc
    13501 ccagttttgt ggttgcgcca ttattagtca tgatatttgc gatcattttg cattggctgc
    13561 cgggcggtgg ctggaatggt ggtgcgctta aattcatgat actgccaatg gtggcgttgt
    13621 cactcgccta tatcgccagt attgcgcgta ttacccgtgg ctctatgatt gaagtattac
    13681 actccaactt tattcgtact gcccgggcga aagggttacc tatgcggcgg atcattttac
    13741 gccacgcatt aaaacctgct ctgttacccg tgctctccta tatgggccct gcatttgtcg
    13801 gcattattac cggttctatg gtcatcgaaa ccatttatgg tttgccgggg attgggcaat
    13861 tgttcgttaa tggtgcattg aaccgtgact attccttagt gttaagcctg accatcctgg
    13921 ttggtgcttt aaccattttg tttaatgcca ttgtcgatgt gctatatgcg gttatcgacc
    13981 cgaaaatccg ttactgatac tggagctcgc gatgatgtta agtaagaaaa acagcgagac
    14041 gctggaaaat ttcagtgaaa agctggaggt cgaagggcgc agcttgtggc aggacgcacg
    14101 tcgacgtttt atgcataacc gtgcggcggt tgccagtctg atagtgctgg tgctgatcgc
    14161 gttatttgtc atcctggcac cgatgctttc gcagtttgcc tatgacgata ctgactgggc
    14221 gatgatgtcc agcgccccgg atatggagtc cggtcactac tttggtactg actcatctgg
    14281 tcgcgacctg cttgtgcgcg ttgcgattgg cgggcgtatc tcactcatgg tcggtgttgc
    14341 tgcggcactg gtggcagtgg tcgtggggac actttatggt tcgctttccg gttatctggg
    14401 cggtaaagtg gattcggtaa tgatgcgtct gctggaaatc ctcaactcct tcccattcat
    14461 gttcttcgtc attttgctgg tgaccttttt cggtcaaaac atcctgctga ttttcgtggc
    14521 gattggcatg gtttcctggc tggatatggc tcgtattgtg cgtgggcaaa ccctgagtct
    14581 gaagcgcaaa gagtttattg aggcggcaca agttggcggt gtatcgacgc cgggcattgt
    14641 tattcgccac attgtgccga acgtactcgg tgtggtggtg gtctacgcat cgctattggt
    14701 gcccagcatg atcctctttg aatctttcct tagcttcctg gggttgggga cgcaagagcc
    14761 gttaagcagc tggggcgcat tgctgagtga tggcgcgaac tcgatggaag tctctccatg
    14821 gttactgttg ttcccagcgg gattcctcgt ggtgacgcta ttttgtttca actttatcgg
    14881 cgatggcttg cgtgatgccc tcgacccgaa agatcgttaa ggagtgcagc catgagcgta
    14941 attgaaactg caactgtgcc gctcgcacaa caacaggctg acgcactgct gaacgtgaaa
    15001 gatttgcgtg tcacctttaa taccccggac ggcgacgtca cggcggtcaa tgatctgaat
    15061 ttttccctac gtgccggaga aacgctgggc attgtaggtg agtctggttc gggtaaatcg
    15121 caaactgcat ttgcgttgat gggcctgttg gctgccaacg gacgtattgg cggatcggca
    15181 accttcaatg ggcgtgaaat cctcaatttg ccagagcgtg aactcaataa actgcgcgct
    15241 gaacaaatct caatgatttt tcaggaccca atgacttcgt tgaatcccta tatgcgcgtc
    15301 ggtgagcagt tgatggaagt gctgatgctg cataagaaca tgagcaaagc tgaggcgttt
    15361 gaagagtcgg tgcggatgct cgatgcggta aaaatgccgg aagcgcgtaa acgcatgaaa
    15421 atgtacccgc acgaattttc tggcggcatg cgtcagcgag ttatgattgc gatggcattg
    15481 ctatgtcgac ctaagctgct gattgcggat gaacccactt ctgcgctgga cgtcaccgta
    15541 caggcgcaga tcatgacgct attgaatgaa ctgaagcggg aatttaatac cgccatcatt
    15601 atgattaccc acgatcttgg cgtggtggcg gggatctgcg acaaagtgct ggtaatgtac
    15661 gccgggcgca cgatggaata tggcaacgcg cgcgatgtct tttatcaacc cgttcatcct
    15721 tattctatcg gtttgctcaa cgcggtgccg cgtctcgatg cggaaggtga aacaatgttg
    15781 accatccctg gtaatccgcc aaacctgctg cgattaccga aaggttgccc gttccagcca
    15841 cgttgtccgc atgcgatgga aatttgtagt agcgcaccgc cgctggaaga gtttacgcct
    15901 ggccgtctgc gtgcttgctt taaaccggtg gaggaactgt tatgaatgct gtaaccgaag
    15961 gaagaaaagt cctcctcgaa atcgccgatc tgaaagtgca ctttgaaatc aaagatggca
    16021 aacagtggtt ctggcaaccg ccgaaaacgc tcaaagccgt cgatggtgta actcttcgcc
    16081 tgtatgaagg ggaaacatta ggtgtggtag gggaatcggg atgcggtaag tccacctttg
    16141 ctcgcgccat catcggtttg gtcaaggcga ccgacggtca tgttgcctgg ttaggtaaag
    16201 agttgctggg catgaagccc gatgaatggc gtgccgttcg cagtgatatt cagatgattt
    16261 tccaggatcc gttggcatcg ctgaacccgc gtatgaccat cggcgagatc atcgctgaac
    16321 cactgcgtac ttatcatccg aaaatgtcac gccaggaagt tcgcgagcgc gtgaaggcga
    16381 tgatgctgaa agtcgggtta ttgcctaacc tgattaaccg ctatccgcat gagttctctg
    16441 gtgggcagtg ccagcgtatc gggattgcac gtgcacttat tcttgaaccg aagctgatta
    16501 tctgtgatga gccggtgtcg gcgctggatg tgtcaattca ggcgcaggtg gtcaacctgc
    16561 tccagcagtt acaacgtgag atgggattgt cattaatttt tatcgctcac gacctggccg
    16621 tggtaaaaca catttccgat cgtgtgttgg tgatgtatct cggccatgcg gtagaactgg
    16681 ggacctatga tgaggtctac cacaatccac tacatcctta caccagggca ttgatgtcgg
    16741 cagtccccat acctgatccg gatctggaga agaacaaaac catccagtta ctggaagggg
    16801 aattaccgtc gccgatcaac ccgccttccg gttgtgtttt ccgtacccgt tgcccgattg
    16861 ccggtccgga gtgcgccaaa acacgtcctg tgctggaggg cagtttcaga cacgccgttt
    16921 cttgcctgaa agtcgatcca ctttaaacac aataagggct gacagttgtc agcccttttt
    16981 cacgccgaaa gcgattattt attcccgcca gatgatatgg caaagtttgt gatctttttc
    17041 gcggcataac agaatgcggg cgaaaacatc gttgatttca ccatcttcac tgtccgccag
    17101 accaatcacc acttcggcaa aaaagtccgg gttcaaatcg aaatcaacat gttcctgcca
    17161 gtcttccgcc ggatcgaata actccgcgcc gccgcgctct tcaaactgaa gattgaacag
    17221 cagaacatca gctggatcga gattgtcggc agccagttcg agaaaaatat cgtaggcctg
    17281 ctcaagcgtt tcatcttcag tcaggcgatt gtttagatcc atatccatag tcactacctg
    17341 tttaacctct gttggcgacg ttttacagca acggactgaa gaagtaaaac agtcgctcgg
    17401 cgacacgttg ccataatgga cgttttagcc ataaacgggc atcgagcaga cgtgaacgcg
    17461 aaatataatc gtcctgaacg gcagcaaggt ctgcaccaaa acctttatcg tcgattgcca
    17521 gggtaatctc gaaatttagc cacagactcc gcatatcaag gttaacggtg ccaaccagac
    17581 ttagttcgcc atcgaccagc acgctcttgg tatgcagtaa cccgccttca aactgataaa
    17641 ttttaacccc agcagcaaga agttccgtaa agaatgcgcg actggcccag ccgaccagca
    17701 tcgagtcatt ttttcgcgga aggataatac tgacatccac cccgcgctgt gccgccgtgc
    17761 aaatcgcatg aagtaaatca tcgcttggca caaagtaggg cgtggtcatg atcaaatatt
    17821 cacgcgccga ataagccgca gtcaataatg cctggtgaat gagatcttcc ggaaagccgg
    17881 ggccagaagc aattgtgtga atggtgtgac cgctggcctg ttcaaacggc ataatattga
    17941 catctggtgg tggtggcaga atacgttttc cggtttcaat ctcccagtcg caggaataaa
    18001 taatccccat cgcggtagcg atggggcctt ccatacgcgc catcagatca atccattgcc
    18061 ctacgcccgc atcttgtttg aagtagcgag ggtcgaccat attcatgctg ccggtgtacg
    18121 cgatgtaatt atcgatcatg atcatcttgc gatgttggcg caggtccata cggcgtaaaa
    18181 acacacgcat cagattgacc tttaaggctt cgaccacttc aataccggca ttacgcatta
    18241 gctcgggcca cgggctgcgg aaaaaagcca cactcccggc ggagtcgagc atcaatcggc
    18301 aatgaatgcc gcgtcgcgca gcggccatta atgattcagc cacctggtcc gccatgccgc
    18361 cgggctgcca gatataaaac accatctcaa tattatggcg agcgagctgg atgtcgcgga
    18421 ttaacgcctg catcacatca tctgactcgg tcatcagttg cagctgattc cctttgaccc
    18481 cagcgatccc ctgacgacgc tcacaaagct tgaatagtgg cgcagcgaca ctgctatttt
    18541 cttcggcgaa gatatgctta caggctttaa ggtcgttaag ccactttgcg gtggaaggcc
    18601 acatcgctct ggcgcgctca gcgcggcgtt tgcctaaatg gagctcgcca acagcaagat
    18661 aggcaataat tccgactaac ggcagaatgt aaataatcaa cagccaggcc atcgcggagg
    18721 gaactgcgcg tcgtttcatt agaatgcgta aagttacgcc tgcaatgagc aaccagtatc
    18781 ccagaatggc caaccaactc accaacgtat aaacggttgt catagattag aaatcctttt
    18841 gaaagcgcat tgttatgagt ttacgcatat ggattcatct ggcaaataaa aacgcgggaa
    18901 aagcactggt cagcgtcggg ggggggggta taatgaccat tctgttattg catagagtag
    18961 ttaacatgaa gcggagtaga acggaagtgg ggcgctggcg catgcagcgt caggctagcc
    19021 gacgtaaatc gcgttggctt gaggggcagt cgcgccgaaa tatgcgtatc cacagcatca
    19081 ggaagtgcat tctaaacaaa cagcgtaact cgttattgtt tgcgatctac aatatctaaa
    19141 tgtactgggc accattgcgg tgcccaataa gtttactctt ccgtctttaa agtctatttt
    19201 tgcgctgatt ctttattgtc agctttcgtt tcttgttcat cgttatcgct gaaaatacca
    19261 tgaccggagt tcaacaacat tgaaacgagc atatcattat taatctcttc accatttaaa
    19321 actcgtgcca gaatttcgct gccaaacagt tgcggcgaga gaatgatatc cggatgtacc
    19381 atcttaatct tattcaggtt tttgctatca ctgacggcga gaactgtttt gacatcactg
    19441 ctcatatctt ttgccgagag tacaacaaac gcgttatctg catcgttatc actcagcgcc
    19501 agaatggccc ggcatcgatc gattcctgct ttctttaata ctgaactgtc attactgtca
    19561 ccggggataa catcagcgtt atcacctaag cgttgctcaa gttgcttgat atcatcttca
    19621 ggcaagttgc tgataaccgt tacattttgt ccgcgttgat tcagttgcag aatcgtattg
    19681 atggcgagaa tcgaatgtcc gcaaacaata aaatgatctt tacgatgcat tgtatgattg
    19741 tttcctttta caagtttgtt gaatcccccg cggataagcg ggccaaaaat tgaagtcatg
    19801 gaggtggcaa aaacggtaat gccggaaata atgaccgaaa tagtgaacaa tcgtgctgat
    19861 tcagaaacag ggacaatatc gccgtagccg acggttgaca tggtttctat cgaaaaatag
    19921 aacgcggtca tcaaactttc tattcgcgga ttaaaacctt cgcttaaata aagcgcaccg
    19981 taggtggagt aaaacagtaa cgtcgtgaaa ctaataaatg cgaaaattgt cccggctgcg
    20041 gcgctactgt gggagaagtc tttgcgcagt atcagcaaaa acaccagcgt aaaaatgcaa
    20101 aatccaatac taaatttcag ccagggataa aaatgcaggg tgtaaattag cgctatcaac
    20161 aacaaaatga tactgattgc ccatgcgagc tttgcccgga acagtaagcc aatggagttc
    20221 aggacaagaa agacgccgag cataaataga ggggcgtttg ccagcaagga gagatccaac
    20281 tctgaaaatg ctttgatatg gaaaatatct agcaaattga ccgacatacc atagattgtt
    20341 ttaaaaataa gcaatccatt taaaaagacg gccagcgcga gaatgtcgtg ccgtagggtc
    20401 acccataaat tagttgctgt ttgtttgaat gtagcccagt gactcactga tacctctgta
    20461 accacaattt tgtttaatta tctcaacgtg cacattttct gaacatacat gcagcgcgtg
    20521 aatgtgttaa acaatagtag atgactttat ccgcagtaat caaaggtttt gagtgagcaa
    20581 ctctgcgtca ggaaagggca ttttcctgaa tatgttcaca tcatataaag aaatacagat
    20641 aaagttatta tctgcttgtg gtggtgaatg cactgaccga cctataagga aaggccaaac
    20701 aagaacacgg ttgcaaaaac cgtgccctta aatattgaat ttctattcag aacactttct
    20761 taaaatgtca ttcagaccat ttcgaagaat ttcgcataaa aacgttctgt tacgttgaac
    20821 ccttacgcca tctgcctttc agcccctaag ccatcacact tcgagtacaa gtgttacatt
    20881 gcgggtatct ttgagcaacg agttttgtgt acacttttgc gtactcgcaa gccagatgtg
    20941 tacacattca atgattaccg acacaaagct caggaagtcc ctgggcaaga aacgagatga
    21001 tatcgaaacc atttcagatt ctcatgggct gaacgcaagg atcagtcagg ctggtaaagt
    21061 ttcttttttc taccgatact gtagtggtca agtaatattg gccacggttt tacagtaaaa
    21121 acggtatctg ttctctgatt cttccggcgt caacccaccg ttgtaatggt gaggcctgac
    21181 gctattgtaa taattcagga tataaccgct aatttgttgc cgggcctcgt ccttgcctac
    21241 gtaaccatcc gtcggcaccc attctgtttt cagactgcgg aagaagcgtt ccataggact
    21301 gttatcccag cagtttcccc gtcggctgac actttgcttt atcctgtaac gccagagaag
    21361 ctgttgatat ttcagtcctg tatactgact tccctggtcg ctatggaaca tgacgtcccg
    21421 cggctgacca cgcacctcat acgccatccg caaggcactg cttatcagtg cagtatcggc
    21481 attcgctgac aggctccagc cgataaccct gcgggcaaaa agatccatga cgaccgccag
    21541 atagcaccag cgatttcctg cccagatata cgtaatatct ccgcaccata ccctgtctgg
    21601 ctccggtaca gcgaactgac gctcga
//
LOCUS       DAMZFT010000081        21386 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.081, whole genome shotgun sequence.
ACCESSION   DAMZFT010000081 DAMZFT010000000
VERSION     DAMZFT010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21386)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21386)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21386
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.081"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..862
                     /locus_tag="PN977_003161"
     CDS             <1..862
                     /locus_tag="PN977_003161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HDI6043171.1"
                     /translation="WATSIVLVVFVGILLLMAFVVLPIAWQQGIYLIRDMPGMLNKLS
                     DFAATLPRRYPALMDAGIIDAMAENMRSRMLTMGDSVVKISLASLVGLLTIAVYLVLV
                     PLMVFFLLKDKEQMLNAVRRVLPRNRGLAGQVWKEMNQQITNYIRGKVLEMIVVGIAT
                     WLGFLLFGLNYSLLLAVLVGFSVLIPYIGAFVVTIPVVGVALFQFGAGTEFWSCFAVY
                     LIIQALDGNLLVPVLFSEAVNLHPLVIILSVVIFGGLWGFWGVFFAIPLATLIKAVIH
                     AWPDGQIAQE"
     gene            complement(899..1747)
                     /locus_tag="PN977_003162"
     CDS             complement(899..1747)
                     /locus_tag="PN977_003162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter"
                     /protein_id="HDI6043172.1"
                     /translation="MRNKLSFDLQLSARKAAIAERIAAHKIARSKVSVFLMAMSAGVF
                     MAIGFTFYLTVIADAPSSQALTHLVGGLCFTLGFILLAVCGTSLFTSSVMTVMAKSRG
                     VISWRTWLINALLVACGNLAGIACFSLLIWFSGLVMSENAMWGVAVLHCAEGKMHHTF
                     TESVSLGIMCNVMVCLALWMSYCGRSLCDKIVAMILPITLFVASGFEHCIANLFVIPF
                     AIAIRHFAPTSFWQLAHSSADHFPALTVSHFITANLLPVMLGNIIGGAVLVSICYRAI
                     YLRQEP"
     gene            complement(1769..3781)
                     /gene="hyfR"
                     /locus_tag="PN977_003163"
     CDS             complement(1769..3781)
                     /gene="hyfR"
                     /locus_tag="PN977_003163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HyfR"
                     /protein_id="HDI6043173.1"
                     /translation="MAMSDEAMFAPPQGITIEAVNGMLAERLAQKHGKASLLRAFIPL
                     PPPFSPVQLIELHVLKSNFYYRYHDDGSDVTATTKYQGEMVDYSRHAVLLGSSGMAEL
                     RFIRTHGSRFTPQDCTLFNWLAHIITPVLQSWLNDEEQQVALRLLEKDRDHHRVLVDI
                     TNAVLSHLDLDNLIADVAREIHHFFGLASVSMVLGDHRKNEKFSLWCSDLSASHCACL
                     PRNMAGDSVLLTQTLQTRQPTLTHRADDLFLWQRDPLLHLLASNGCESALLIPLTFGN
                     HTPGALLLAHTSSTLFSEENCQLLQHIADRIAIAVGNADAWRSMTDLQESLQQENHQL
                     SEQLLSNLGVGDIIYQSQAMEDLLQQVDIVAKSDSTVLICGETGTGKEVIARAIHQLS
                     PRRDKPLVKINCAAIPASLLESELFGHDKGAFTGAINTHRGRFEIADGGTLFLDEIGD
                     LPLELQPKLLRVLQEREIERLGGSRTIPVNVRVIAATNRDLWQMVEDRQFRSDLFYRL
                     NVFPLELPPLRDRPEDIPLLAKHFTQKMARHMNRAIDAIPTEALRQLMSWDWPGNVRE
                     LENVIERAVLLTRGNSLNLHLNVRQSRLLPTLNEDSALRSSMAQLLHPTTPENDEEER
                     QRIVQVLRETNGIVAGPRGAATRLGMKRTTLLSRMQRLGISVREVL"
     gene            complement(3811..4224)
                     /gene="hyfJ"
                     /locus_tag="PN977_003164"
     CDS             complement(3811..4224)
                     /gene="hyfJ"
                     /locus_tag="PN977_003164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416985.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 assembly chaperone HyfJ"
                     /protein_id="HDI6043174.1"
                     /translation="MTEECGEIVFWTLRKKFVASSDEMPEHSSQVMYYSLAIGHHVGV
                     IDCLNVAFRCPLTEYEDWLALVEEEQARRKMLGVMTFGEIVIDASHTALLTRAFAPLA
                     DDATSAWQARSIQFIHLLDEIVQEPAIYLMARKIA"
     gene            complement(4217..4975)
                     /gene="hyfI"
                     /locus_tag="PN977_003165"
     CDS             complement(4217..4975)
                     /gene="hyfI"
                     /locus_tag="PN977_003165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfI"
                     /protein_id="HDI6043175.1"
                     /translation="MSPVLTQHVSQPITLDEQTQKMKQHLLQDIRRSAYVYRVDCGGC
                     NACEIEIFAAITPVFDAERFGIKVVSSPRHADILLFTGAVTRAMRMPALRAYESAPDH
                     KICVSYGACGVGGGIFHDLYSVWGGSDTIVPIDVWIPGCPPTPAATIHGFAVALGLLQ
                     QKIHAVDYRDPTGVTMQPLWPQIPPSQRIAIEREARRLAGYRQGREICDRLLRHLSDD
                     PTGNRVNTWLRDADDPRLNSIVQQLFRVLRGLHD"
     gene            complement(4972..5517)
                     /gene="hyfH"
                     /locus_tag="PN977_003166"
     CDS             complement(4972..5517)
                     /gene="hyfH"
                     /locus_tag="PN977_003166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit H"
                     /protein_id="HDI6043176.1"
                     /translation="MLKLLKTIMRAGTATVKYPFAPLEVSPGFRGKPDLMPSQCIACG
                     ACACACPANALTIQTDDQQNTRTWQLYLGRCIYCGRCEEVCPTRAIQLTNNFELTVTN
                     KADLYTRATFHLQRCSRCERPFAPQKTVALAAELLAQQQNAPQNREMLRAQASVCPEC
                     KQRATLINDDTNEPLVAKEQL"
     gene            complement(5527..7242)
                     /gene="hyfG"
                     /locus_tag="PN977_003167"
     CDS             complement(5527..7242)
                     /gene="hyfG"
                     /locus_tag="PN977_003167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfG"
                     /protein_id="HDI6043177.1"
                     /translation="MNVNSSSNRGEAILAALKTQFPGAVLDEERQTPEQVTITVKINL
                     LPDVVHYLYYQHDGWLPVLFGNDERTLNGHYAVYYALSMEGAEKCWIVVKALVDADSR
                     EFPSVTPRVPAAVWGEREIRDMYGLIPVGLPDQRRLVLPDDWPEDMHPLRKDAMDYRL
                     RPEPTTDTETYPFINEGNSDARVIPVGPLHITSDEPGHFRLFVDGEQIVDADYRLFYV
                     HRGMEKLAETRMGYNEVTFLSDRVCGICGFAHSVAYTNSVENALGIEVPQRAHTIRSI
                     LLEVERLHSHLLNLGLSCHFVGFDTGFMQFFRVREKSMTMAELLTGSRKTYGLNLIGG
                     VRRDILKEQRLQTLKLVREMRADVSELVEMLLATPNMEQRTQGIGILDRQIARDYSPV
                     GPLIRGSGFARDLRFDHPYADYGNIPKTLFTFTGGDVFSRVMVRVKETFDSLAMLEFA
                     LDNMPDTPLLTEGFSYKPHAFALGFVEAPRGEDVHWSMLGDNQKLFRWRCRAATYANW
                     PVLRYMLRGNTVSDAPLIIGSLDPCYSCTDRVTLVDVRKRQSKTVPYKEIERYGIDRN
                     RSPLK"
     gene            complement(7232..8812)
                     /gene="hyfF"
                     /locus_tag="PN977_003168"
     CDS             complement(7232..8812)
                     /gene="hyfF"
                     /locus_tag="PN977_003168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit F"
                     /protein_id="HDI6043178.1"
                     /translation="MSYSVMFALLLLTPLLFSLLCFACRKRGHSPTRAVTVLHSLGIT
                     LLLILALWVVQTAADAGEIFAAGLWLHIDGLGGLFLAILGVIGFLTGVYSIGYMRHEV
                     EHGELSPVTLCDYYGFFHLFLFTMLLVVTSNNLIVMWAAIEATTLSSAFLVGIYGQRS
                     SLEAAWKYIIICTVGVAFGLFGTVLVYANAASVMPQAEMAIFWSEVLKQSSLLDPTLM
                     LLAFVFVLIGFGTKTGLFPMHAWLPDAHSEAPSPVSALLSAVLLNCALLVLIRYYIII
                     CQAIGSDFPNRLLLIFGMLSVAVAAFFILVQRDIKRLLAYSSVENMGLVAVALGIGGP
                     LGIFAALLHTLNHSLAKTLLFCGSGNVLLKYGTRDLNVVCGMLKIMPFTAVLFGGGAL
                     ALAGMPPFNIFLSEFMTVTAGLARNHLLLIVLLLLLLTLVLAGLVRMAARVLMAKPPQ
                     AVNRGELGWLTTSPMVILLVMMLAMGTHIPQPVIRILAGASTIVLSGTHDLPAQRSTW
                     HDFLPSGTASVSEKHSER"
     gene            complement(8817..9467)
                     /gene="hyfE"
                     /locus_tag="PN977_003169"
     CDS             complement(8817..9467)
                     /gene="hyfE"
                     /locus_tag="PN977_003169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 membrane subunit"
                     /protein_id="HDI6043179.1"
                     /translation="MTGSMIVNNLAGLMMLTSLFVISVKSYRLSCGFYACQSLVLVSI
                     FATLSCLFAAEQLLIWSASAFITKVLLVPLIMTYAARNIPQNLPEKALFGPAMMTLLA
                     ALIVLLCAFVVQPVKLPMATGLKPALAVALGHFLLGLLCIVSQRNILRQIFGYCLMEN
                     GSHLVLALLAWRAPELVEIGIATDAIFAVIVMVLLARKIWRTHGTLNVNNLTALKG"
     gene            complement(9479..10918)
                     /gene="hyfD"
                     /locus_tag="PN977_003170"
     CDS             complement(9479..10918)
                     /gene="hyfD"
                     /locus_tag="PN977_003170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfD"
                     /protein_id="HDI6043180.1"
                     /translation="MENLALTTLLLPFIGALVVSFSPQRRAAEWGILFAALTTLCMLS
                     LISAFYQADKVAVTLTLVNVGDVALFGLVIDRVSTLILFVVVFLGLLVTIYSTGYLTD
                     KNREHPHNGTNRYYAFLLVFIGAMAGLVLSSTLLGQLLFFEITGGCSWALISYYQSDK
                     AQRSALKALLITHIGSLGLYLAAATLFLQTGTFALSAMSELHGDARYLVYGGILFAAW
                     GKSAQLPMQAWLPDAMEAPTPISAYLHAASMVKVGVYIFARAIIDGGNIPHVIGGVGM
                     VMALVTILYGFLMYLPQQDMKRLLAWSTITQLGWMFFGLSLSIFGSRLSLEGSIAYIV
                     NHAFAKSLFFLVAGALSYSCGTRLLPRLRGVLHTLPLPGVGFCVAALAITGVPPFNGF
                     FSKFPLFAAGFSLSVEYWILLPAMILLMIESVASFAWFIRWFGRVVPGKPSEAVADAA
                     PLPGSMRLVLIVLIVMSLISSVIAATWLQ"
     gene            complement(10935..11882)
                     /gene="hyfC"
                     /locus_tag="PN977_003171"
     CDS             complement(10935..11882)
                     /gene="hyfC"
                     /locus_tag="PN977_003171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416978.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfC"
                     /protein_id="HDI6043181.1"
                     /translation="MRQTLCDGYLIIFALAQAVILLALTPLFTGISRQIRARMHSRRG
                     PGIWQDYRDIHKLFKRQEVAPTSSGLMFRLMPWVLISSMLVLAMALPLFITVSPFAGG
                     GDLITLIYLLALFRFFFALSGLDTGSPFAGIGASRELTLGILVEPMLILSLLVLALIA
                     DSTHIEMISKTLATGWNSPLTTVLALLACGFACFIEMGKIPFDVAEAEQELQEGPLTE
                     YSGAGLALAKLGLGLKQVVMASLFVALFLPFGRAQELSLTCLLTSLVVTLLKVLLIFV
                     LASIAENTLARGRFLLIHHVTWLGFSLAALAWVFWLTGL"
     gene            complement(11893..13911)
                     /gene="hyfB"
                     /locus_tag="PN977_003172"
     CDS             complement(11893..13911)
                     /gene="hyfB"
                     /locus_tag="PN977_003172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit B"
                     /protein_id="HDI6043182.1"
                     /translation="MDALQLLTWSLILYLFASLASLFLLGLDRLAIKLSGITSLVGGV
                     IGIISGITQLHAGVTLVARFATPFDFADLTLRMDSLSAFMVLVISLLVVVCSLYSLTY
                     MREYEGKGAAAMGFFMNLFIASMVALLVMDNAFWFIVLFEMMSLSSWFLVIARQDKTS
                     INAGMLYVFIAHAGSVLIMIAFLLMGRESGSLDFASFRTLSLSPGLASAVFLLAFFGF
                     GAKAGMMPLHSWLPRAHPAAPSHASALMSGVMVKIGIFGILKVAMDLLAQTGLPLWWG
                     ILVMAIGAISALLGVLYALAEQDIKRLLAWSTVENVGIILLAVGVSMVGLSLHDPLLT
                     MVGLLGALFHLLNHALFKGLLFLGAGAIISRLHTHDMEKMGALAKRMPWTAAACLIGC
                     LAISAIPPLNGFISEWYTWQSLFSLSRVEAVALQLAGPIAMVMLAVTGGLAVMCFVKM
                     YGITFCGAPRSTHAEEAQDVPNTMIVAMLLLAALCVFIALSASWLAPKIMHIAHAFTN
                     TPPVTVASGIALVPGTFHTRVTPSLLLLLLLAMPLLPGLYWLWCRSRRAAFRRTGDAW
                     ACGYGWDNAMAPSGNGVMQPLRVVFCALFRLRQQLDPTLRLNKGLAHVTARAQSTEPF
                     WDERVIRPIVSATQRLAKEIQHLQSGDFRLYCLYVVAALVVLLIAIAV"
     gene            complement(13911..14528)
                     /gene="hyfA"
                     /locus_tag="PN977_003173"
     CDS             complement(13911..14528)
                     /gene="hyfA"
                     /locus_tag="PN977_003173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311370.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfA"
                     /protein_id="HDI6043183.1"
                     /translation="MNRFVVAEPLWCTGCNTCLAACSDVHKTQGLQQHPRLALAKTST
                     LTAPVVCHHCEEAPCLQVCPVNAISQRDDAIQLNESLCIGCKLCAVVCPFGAISASGS
                     RPVNAPAQYVFQAEGSLKDGEENVSTQHALLRWEPGVQTVAVKCDLCDFLPEGPACVR
                     ACPNQALRLITDDRLQRQMKEKQRLAASWFANGGEDPLSLTQEQR"
     gene            complement(14781..15251)
                     /gene="bcp"
                     /locus_tag="PN977_003174"
     CDS             complement(14781..15251)
                     /gene="bcp"
                     /locus_tag="PN977_003174"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HDI6043184.1"
                     /translation="MNPLKAGDIAPKFSLPDQDGEQVNLTDFQGQRVLVYFYPKAMTP
                     GCTVQACGLRDNMDELKKAGVDVLGISTDKPEKLSRFAEKELLNFTLLSDEDHQVCEQ
                     FGVWGEKSFMGKTYDGIHRISFLIDADGKIEHVFDDFKTSNHHDVVLNWLKEHA"
     gene            complement(15251..15823)
                     /locus_tag="PN977_003175"
     CDS             complement(15251..15823)
                     /locus_tag="PN977_003175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     repressor"
                     /protein_id="HDI6043185.1"
                     /translation="MTLSSQHYLVITALGADRPGIVNTITRHVSSCGCNIEDSRLAML
                     GEEFTFIMLLSGSWNAITLIESTLPLKGAELDLLIVMKRTTARPRPPMPASVWVQVDV
                     ADSPHLIERFTALFDAHHMNIAELVSRTQPAENERAAQLHIQITAHSPASADAANIEQ
                     AFKALCTELNAQGSINVVNYSQHDEQDGVK"
     gene            15969..16847
                     /gene="dapA"
                     /locus_tag="PN977_003176"
     CDS             15969..16847
                     /gene="dapA"
                     /locus_tag="PN977_003176"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001311023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HDI6043186.1"
                     /translation="MFTGSIVAIVTPMDEKGNVCRASLKKLIDYHVASGTSAIVSVGT
                     TGESATLNHDEHADVVMMTLELADGRIPVIAGTGANATAEAISLTQRFNDSGIVGCLT
                     VTPYYNRPSQEGLYQHFKAIAEHTDLPQILYNVPSRTGCDLLPETVGRLAKVKNIIGI
                     KEATGNLTRVNQIKELVSDDFVLLSGDDASALDFMQLGGHGVISVTANVAARDMAQMC
                     KLAAEGHFAEARVINQRLMPLHNKLFVEPNPIPVKWACKELGLVATDTLRLPMTPITD
                     SGRETVRAALKHAGLL"
     gene            16864..17898
                     /gene="bamC"
                     /locus_tag="PN977_003177"
     CDS             16864..17898
                     /gene="bamC"
                     /locus_tag="PN977_003177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamC"
                     /protein_id="HDI6043187.1"
                     /translation="MAYSVQKSRLAKVAGVSLVLLLAACSSDSRYKRQVSGDEAYLEA
                     APLAELHAPAGMILPVTSGDYAIPVTNGSGAVGKALDIRPPAQPLALVSGARTQFTGD
                     TASLLVENGRGNTLWPQVVSVLQAKNYTITQRDDAGQTLTTDWVQWNRLDEDEQYRGR
                     YQISVKPQGYQQAVTVKLLNLEQAGKPVADAASMQRYSTEMMNVISAGLDKSATDAAN
                     AAQNRASTTMDVQSAADDTGLPMLVVRGPFNVVWQRLPAALEKVGMKVTDSTRSQGNM
                     AVTYKPLSDSDWQELGASDPGLASGDYKLQVGDLDNRSSLQFIDPKGHTLTQSQNDAL
                     VAVFQAAFSK"
     gene            18111..18824
                     /gene="purC"
                     /locus_tag="PN977_003178"
     CDS             18111..18824
                     /gene="purC"
                     /locus_tag="PN977_003178"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HDI6043188.1"
                     /translation="MQKQAELYRGKAKTVYSTENPDLLVLEFRNDTSAGDGARIEQFD
                     RKGMVNNKFNYFIMSKLAEAGIPTQMERLLSDTECLVKKLDMVPVECVVRNRAAGSLV
                     KRLGIEEGIELNPPLFDLFLKNDAMHDPMVNESYCETFGWVSKENLARMKELTYKAND
                     VLKKLFDDAGLILVDFKLEFGLYKGEVVLGDEFSPDGSRLWDKETLEKMDKDRFRQSL
                     GGLIEAYEAVARRLGVQLD"
     gene            18992..19855
                     /locus_tag="PN977_003179"
     CDS             18992..19855
                     /locus_tag="PN977_003179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HDI6043189.1"
                     /translation="MRWQGRRESDNVEDRRNSSGGPSMGGPGFRLPSGKGGLILLIVV
                     LVAGYYGVDLTGLMTGQPVSQQQSTRSISPNEDEAAKFTSVILATTEDTWGQQFEKMG
                     KTYQQPKLVMYRGMTRTGCGAGQSIMGPFYCPADGTVYIDLSFYDDMKDKLGADGDFA
                     QGYVIAHEVGHHVQKLLGIEPKVRQLQQNATQAEVNRLSVRMELQADCFAGVWGHSMQ
                     QQGVLETGDLEEALNAAQAIGDDRLQQQSQGRVVPDSFTHGTSQQRYSWFKRGFDSGD
                     PAQCNTFGKSI"
     gene            19870..>21386
                     /gene="tmcA"
                     /locus_tag="PN977_003180"
     CDS             19870..>21386
                     /gene="tmcA"
                     /locus_tag="PN977_003180"
                     /EC_number="2.3.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cytosine(34) acetyltransferase TmcA"
                     /protein_id="HDI6043190.1"
                     /translation="MAELTALHTLTAQMKREGIRRLLVLSGEEGWCFDHALKLRDALP
                     GDWLWISPQPDAENHCSPSALQTLLGREFRHAVFDARHGFDAAAFAALSGTLKAGSWL
                     VLLLPVWDEWENQPDADSLRWSDCPDPIATPHFVQHFKRVLTANNDAILWRQNQPFSL
                     AHFTPRTDWHPATGAPQPEQQQLLQQLLTMPLGVAVVTAARGRGKSALAGQLISRIAG
                     SAIVTAPAKAATYVLAQFAGEKFRFIAPDALLASDEQADWLVVDEAAAIPAPLLHQLV
                     SRFPRTLLTTTVQGYEGTGRGFLLKFCARFPHLHRFELQQPIRWAQGCPLEKMVSEAL
                     VFDDENFTHEPQGDIVISAFEQTLWRSEPETPLKVYQLLSGAHYRTSPLDLRRMMDAP
                     GQHFLQAAGENEIAGALWLVDEGGLSQQLSQAVWAGFRRPRGNLVAQSLAAHGSNPLA
                     ATLRGRRVSRIAVHPARQREGTGRQLIAGALQYTHDLDYLSVSFGYTGELWRFWQR"
BASE COUNT         5320 a         5671 c         5801 g         4594 t
ORIGIN      
        1 ctgggcgacg tcgattgtat tggtggtttt cgtcggtata ttgctactga tggcgttcgt
       61 ggtgctgcct atcgcctggc aacagggcat ctacttaatc cgcgatatgc cggggatgct
      121 caataagctt tctgactttg ccgccacgtt gccgcgccgc tatccggcgt taatggacgc
      181 gggcattatt gatgcaatgg ccgaaaatat gcgcagtcgg atgctgacca tgggcgattc
      241 ggtggtgaaa atttccctcg cctcgctggt cggtttgctg accatagccg tctatctggt
      301 gctggtgcca ttgatggtct tcttcctgct gaaagacaaa gagcagatgc tgaacgccgt
      361 tcgtcgggtg ctgccgcgta accgtgggct ggcaggacag gtgtggaagg agatgaatca
      421 acaaatcacc aactatatcc gcggcaaagt gctggagatg atcgtggtgg ggatcgccac
      481 ctggctgggg ttcttactct tcgggctgaa ctattcgctg ctgctggcgg tgctggtcgg
      541 cttctcggta cttattccgt acattggtgc atttgtggtg accattccgg tggttggcgt
      601 ggcgctattc cagtttggtg ctggcacgga attctggagc tgcttcgcgg tgtatctgat
      661 tattcaggcg ctggacggca acctgttagt acctgtgttg ttctccgaag cggttaacct
      721 gcatccgctg gtcattattt tatcagtagt gatcttcggt ggtttgtggg gattctgggg
      781 cgtattcttc gccattccat tggcgacgct gatcaaagcc gtgattcacg cctggcccga
      841 tgggcaaatc gcgcaagaat aatcttcagc ccgacgaact gtttcgccgg gctgattttt
      901 agggttcctg acgtaaataa atagcccgat aacaaatact caccagcact gcaccgccga
      961 taatattacc cagcatcacc gggagcagat tggcggtaat aaaatggctg accgtcagtg
     1021 ccggaaaatg gtctgcgcta ctgtgcgcca gttgccagaa ggaggtaggg gcgaaatggc
     1081 gaatggcaat ggcgaacgga atcacaaaca aattggcgat acagtgctca aaaccactgg
     1141 cgacaaacag ggtgatgggc aaaatcatgg cgacgatttt gtcgcataac gaacgtccgc
     1201 aataactcat ccacagcgcc aggcaaacca tcacattgca cataatgccg aggctgactg
     1261 attcagtaaa tgtatgatgc attttgccct cggcgcagtg taaaaccgcg actccccaca
     1321 tcgcgttttc actcatcacc agcccggaaa accaaattaa caaactgaaa caggcaatac
     1381 ctgccagatt accgcaggcc accagaagtg cattaatcag ccatgttcgc cagctaataa
     1441 cgccccgact ttttgccatc accgtcatta ccgacgaggt gaacaggctg gtgccgcaaa
     1501 ccgccagcaa aataaagccg agcgtaaagc aaaggcctcc caccagatgg gttaatgcct
     1561 gtgaagacgg ggcatcggcg ataacggtaa ggtaaaaagt aaatccgatc gccataaaca
     1621 cgcctgcgga catcgccatt aaaaagaccg acactttgct gcgggcaatt ttatgggcgg
     1681 caatccgttc agcgattgcc gcttttctgg cgctcaactg caagtcgaaa gagagtttgt
     1741 ttctcatgaa tactcctgca aaagcagatt acaacacctc gcgaaccgag atccccagac
     1801 gctgcattcg tgacagcagc gtggtgcgct tcatccctaa tcgtgtcgcc gcgccacggg
     1861 gcccggcaac aatgccattg gtttctcgca atacctgaac aatgcgctga cgttcttctt
     1921 cgtcattctc tggcgtcgtc gggtgcagca actgcgccat tgaactgcga agcgctgaat
     1981 cttcatttag cgtcggcagt aaacggcttt gtcggacatt aagatgtaaa ttcagactgt
     2041 taccgcgagt cagtagtacc gctcgctcaa tcacgttttc cagctcgcgc acgttgcccg
     2101 gccagtccca cgacatcaac tggcgtagcg cctcggtcgg gatggcgtca attgcgcgat
     2161 tcatatggcg cgccattttt tgcgtgaaat gctttgctaa aagagggata tcttccggac
     2221 ggtcgcgcag tggcggcagt tccagtggga agacattcag gcgataaaag agatcgctgc
     2281 gaaactggcg atcttcaacc atttgccaca aatcacggtt ggtggcggca atgactctga
     2341 catttaccgg gatcgttcta ctcccgccga gacgctcaat ctcccgttcc tgcaatacgc
     2401 gcagcagttt aggctgaagt tctaacggca gatcgccaat ttcatcgaga aacaacgtgc
     2461 cgccatcggc aatttcaaaa cgaccacgat gggtattaat cgcaccagta aatgcccctt
     2521 tgtcatgacc gaataactca ctttccagaa gactggcggg gatggcagcg cagttgattt
     2581 tgaccagtgg cttgtcgcgt cgtgggctaa gttgatggat cgctctggcg atcacctctt
     2641 tgccggttcc ggtttcacca caaatcaaca ccgtactgtc gctcttcgcc acaatatcta
     2701 cctgctggag caggtcttcc attgcctggc tttgatagat aatgtcaccg acgcccagat
     2761 tcgaaaggag ctgctcgcta agctggtggt tttcttgctg caagctttcc tgcaaatcgg
     2821 tcatgctgcg ccaggcatcg gcattgccaa cggcaatagc gatgcgatcg gcaatatgtt
     2881 gtagtagctg gcagttttcc tcactaaaga gagtggaaga ggtatgcgcc agcaacaatg
     2941 cacccggtgt atggttgcca aaggtgagcg gtataaggag cgcagattcg cagccgttag
     3001 atgcaagtaa gtgtaataac gggtcgcgtt gccagagaaa cagatcgtct gcacggtgcg
     3061 tcaaggtcgg ttgtcgggtt tgtagcgttt gtgtcagcaa tacactgtcg ccagccatat
     3121 tgcgtggcag acacgcacaa tgtgaggcag aaagatcgct gcaccacagg ctgaacttct
     3181 cgttctttcg atgatcgccc agtaccatac tgactgaagc cagaccgaaa aaatgatgga
     3241 tctcacgagc gacgtcagcg atcagattgt cgagatcaag atgtgacaac actgcattag
     3301 tgatatccac cagtacccga tgatgatcgc gatctttctc cagcaaacgc agcgccacct
     3361 gctgttcttc atcattgagc catgattgca gaaccggagt gattatgtgt gccagccagt
     3421 taaacagtgt gcaatcctgg ggagtaaaac gactgccgtg ggtgcgaata aagcgtagct
     3481 ccgccattcc actactgccg agaaggacgg cgtggcgcga ataatcgacc atctcaccct
     3541 gatactttgt tgttgccgtc acatcgctgc catcatcatg gtagcggtaa tagaagttgc
     3601 ttttgagaac atgcagttca ataagttgta ccgggctgaa cggcggcggc agcgggatga
     3661 aggcgcgtaa taaagacgcc ttgccgtgtt tctgcgctaa ccgctccgcg agcattccgt
     3721 ttaccgcttc aattgttatt ccttgcggcg gggcaaacat cgcctcgtct gacatagcca
     3781 tcaacagtcc ttattaatga gaaatccttc tcacgcaatt tttctggcca tcagatagat
     3841 ggccggttcc tgcacaattt catccaacag atgaatgaat tgaatgctac gcgcctgcca
     3901 cgccgacgtc gcgtcatctg ccagtggcgc gaatgcccgg gtcaacaggg cggtgtggct
     3961 ggcgtcaata acaatctcac caaaagtcat cacccccagc atcttacgtc gggcttgctc
     4021 ctcttcgacc agtgcaagcc aatcttcgta ttccgtcagt gggcagcgga aggcgacatt
     4081 cagacaatca atcacgccaa cgtgatggcc gatagctagc gagtaataca ttacctgaga
     4141 gctgtgttcc ggcatctcgt cgctactggc gacaaacttt tttcgcagcg tccagaaaac
     4201 aatttccccg cactcttcag tcatgtaacc cccggagtac gcgaaagagt tgctgaacga
     4261 tactattgag acgtggatcg tcggcatcgc gcaaccaggt gttaacccga tttcctgtag
     4321 gatcgtcgct taaatggcgc aggagtcgat cgcaaatttc tcgcccttga cgatagcccg
     4381 ccagccgcct cgcttctcgc tcaatggcga tacgctgtga tggcgggatc tgcggccaca
     4441 gcggttgcat ggtcaccccg gtgggatcgc gataatccac cgcgtgaatc ttctgttgca
     4501 gcaaaccgag cgccacggcg aaaccgtgaa tggtggcggc cggtgttggc gggcagccgg
     4561 ggatccaaac atcaatgggg acaatggtgt cgctgccgcc ccagacgctg taaagatcgt
     4621 ggaaaatacc gccgccgaca ccgcacgcgc cgtaggaaac acaaatttta tgatcggggg
     4681 cagactcata tgcccgaagt gcaggcatac gcatcgcacg ggtgactgcg ccagtaaata
     4741 acaaaatatc ggcgtgacgc ggtgatgaaa caaccttaat gccaaaacgt tctgcgtcga
     4801 atacgggtgt gatggcggca aaaatttcga tttcgcaggc gttacagccg ccgcaatcga
     4861 cgcgataaac gtaagccgag cgacggatat cctgtagcaa atgctgcttc atcttttgcg
     4921 tttgctcgtc cagcgtaatg ggctggctga catgttgcgt aagcactgga ctcatagctg
     4981 ctccttagcc accagcggtt catttgtgtc gtcgttgatc agcgtcgcgc gttgtttgca
     5041 ttccgggcag acgctcgctt gcgcccgtaa catttcgcgg ttttgtggtg cattttgttg
     5101 ctgtgccaac agttctgcag ccagtgcgac ggttttttgc ggggcaaacg ggcgttcgca
     5161 acggctgcaa cgttgtagat ggaacgtcgc gcgggtatag agatcggctt tattggtgac
     5221 ggtcagttca aagttattgg taagctgaat ggctctggtc gggcacactt cttcacaacg
     5281 tccgcagtaa atacaacgcc ccagatagag ctgccaggtg cgcgtatttt gctggtcgtc
     5341 ggtctggata gtcagcgcat ttgccggaca agcacaggcg caggcaccgc aggcaataca
     5401 ttggctgggc atcaggtccg gtttcccgcg aaagcctggg ctgacctcca gtggcgcgaa
     5461 gggatacttc accgtcgcgg ttccggcgcg cataatagtt ttcagtaact tcagcatttt
     5521 ctgtccttac ttcagcggcg aacggttacg atcaatgccg tagcgttcga tctctttata
     5581 cggcacggtt tttgactggc gcttgcgcac atcaaccagc gtcacacggt cggtacagga
     5641 gtaacaggga tcaaggctac cgataatcag cggtgcgtca gaaacggtat tgccgcgcag
     5701 catgtaacgc agcactggcc agttggcgta ggtggcggca cgacagcgcc agcggaacaa
     5761 tttttggtta tcaccgagca tgctccagtg cacgtcttca ccgcgtggcg cttcaacaaa
     5821 gcccagcgcg aatgcgtgag gtttatagct aaagccttcg gtcagcagtg gggtatccgg
     5881 catgttgtcg agggcaaatt ccagcattgc cagcgaatca aacgtctctt tgacacggac
     5941 catcacgcgg gagaagacat cgccgccggt aaaggtaaac agcgttttgg gaatattacc
     6001 gtagtcggcg taggggtgat caaagcgcaa atcacgggca aaaccactgc cgcggatcag
     6061 cggcccgaca gggctataat cacgggcgat ttgtcggtcg agaatgccaa tgccctgagt
     6121 gcgttgttcc atattcggcg tggcaagcag catctctacc agctccgaca cgtcggcgcg
     6181 catctcgcgc accagtttca gcgtttgcag acgttgctct ttgagaatat cgcggcgaac
     6241 accaccaatc agattcagac cgtaggtttt acgcgacccg gtcagcaatt ccgccatcgt
     6301 catcgacttt tcccgcacgc ggaaaaattg cataaagccg gtatcaaaac caacaaaatg
     6361 gcaggagagg ccaaggttga gcaaatgact atgcagccgt tcgacttcca gcagaatcga
     6421 gcgaatagta tgcgctcgtt gcggcacctc aatccccagt gcattttcaa ccgagttggt
     6481 ataggccaca ctgtgggcaa aaccgcaaat cccacacacg cggtcggata agaaggtcac
     6541 ttcgttgtag cccatccgcg tttctgccag tttctccatg ccgcgatgga cataaaacag
     6601 gcggtaatca gcatcgacaa tttgctcgcc atccacaaac aagcggaagt gacctggttc
     6661 atcggaggtg atatgcagcg ggccgacagg gatcacccgc gcatcgctgt tgccctcgtt
     6721 gataaacgga tacgtttcgg tatcagtcgt cggttctggg cgcagtcgat aatccatcgc
     6781 gtctttgcgc agcggatgca tatcttccgg ccagtcatcg ggcaacacca gccgacgctg
     6841 atccggcagg ccaaccggaa tcagcccgta catatcgcga atttctcgct cgccccagac
     6901 cgcggcaggg acgcgcggtg tgactgacgg aaactcccga ctgtcggcat cgaccagtgc
     6961 cttcaccacg atccagcatt tttcggcccc ttccatagaa agggcataat aaaccgcgta
     7021 atgaccgtta agtgtccgct cgtcgttgcc aaacaggact ggaagccagc catcatgttg
     7081 ataataaaga taatgcacaa cgtcaggcag cagattgatt ttcaccgtaa tggtgacctg
     7141 ttcaggcgtt tgtcgctctt catccagcac cgcgccgggg aactgcgttt tcagggcggc
     7201 gagaatcgct tcgccacgat ttgacgatga attaacgttc actgtgtttc tccgaaacag
     7261 atgcggtgcc tgaaggcaaa aaatcatgcc aggtgctacg ttgtgcaggc aggtcgtgcg
     7321 tccctgagag gactatagtg gaagcgcccg ccaggatcct gatgacaggt tgtggaatat
     7381 gcgttcccat cgccagcatc atgaccagca gaatcaccat tggcgaggtg gtcaaccagc
     7441 caagttcacc ccggttaacg gcctgcggcg gtttcgccat taacacccgc gcagccatcc
     7501 gtaccaggcc cgccagcacc agcgttaaca gcaataacag caggacgata agcagcagat
     7561 gattacgcgc cagccctgcg gtaacggtca taaattcgct aagaaaaatg ttgaagggcg
     7621 gcatcccggc cagcgccagc gcaccgccgc caaacagcac ggcggtaaat ggcatgattt
     7681 tgagcattcc gcagacgacg ttgagatcgc gcgtgccgta cttgagcagt acattgccgg
     7741 aaccgcagaa cagcagcgtt tttgccagac tgtggtttaa ggtgtgcagc agcgcggcaa
     7801 aaattcccag cggcccgcca atgcctaacg ccaccgcgac cagtcccatg ttctccacgc
     7861 tggagtacgc cagcagacgc ttaatgtccc gctgtaccag aatgaaaaat gccgccacgg
     7921 caaccgacaa catgccgaag atgagcaaca accggttggg gaaatcgctg ccgatggcct
     7981 ggcaaataat gatgtaatag cgaatcagca ccaacagcgc gcagttcagc aatacggcgg
     8041 agagcagggc gctgaccgga ctcggcgctt cactgtgagc atctggcagc caggcgtgca
     8101 tggggaatag cccggtttta gtgccaaagc caattagcac aaacacaaag gccaacagca
     8161 ttaatgttgg gtcaagcaag gacgattgct taagaacctc gctccagaat atcgccattt
     8221 ctgcctgcgg cataacgctg gcggcgttgg cgtataccag cacggtaccg aacagaccaa
     8281 aagcgacacc aacagtacaa ataatgatgt acttccatgc agcttccagc gatgaacgct
     8341 gaccgtaaat gcctaccaga aacgccgagc ttaaggtggt ggcttcgatc gccgcccaca
     8401 tcacaatcag gttattgctg gtaacaacca gcagcatggt gaacaaaaac agatggaaga
     8461 aaccgtagta atcgcacagc gtaacgggtg aaagctcgcc gtgctccact tcatgacgca
     8521 tgtagccaat cgagtaaaca ccggtgagaa agccaatcac accaagaatg gcgaggaaca
     8581 aaccgcccag accatcaata tgaagccaca gtcccgcagc gaatatttct cccgcatcag
     8641 cggctgtttg gaccacccag agtgccagaa tcagcagcag tgtgatccct aagctatgta
     8701 atactgtcac cgcgcgagtc ggagagtgcc cccgtttccg gcaggcaaaa cagagcagcg
     8761 aaaaaagcag cggcgtgagc aggagtaaag cgaacatcac agaataactc atctcattat
     8821 cccttcagcg cggtcaagtt gttcacgttc agcgtgccgt gggtacgcca tatttttctt
     8881 gccagtaaca ccatcacaat gacggcgaag atggcgtcgg tagcgatacc aatttccacc
     8941 agttccggtg cacgccaggc aagaagcgcc agcaccagat gggagccgtt ttccatcagg
     9001 cagtaaccaa aaatttgccg caggatattg cgctggctga caatgcacag caggccaagc
     9061 agaaaatgac ctaacgccac cgccagcgcc ggtttcagcc cggtagccat cggtagcttc
     9121 acgggctgaa cgacaaatgc gcaaagcagg acaattaacg ccgcgagcag tgtcatcatt
     9181 gccggaccga ataacgcttt ttccgggagg ttctggggaa tatttcgtgc agcgtaagtc
     9241 atgattaacg gtaccagcag cactttggtg ataaaggcgc tggcggacca gatcagcagt
     9301 tgctctgcgg cgaacaggca cgagagagtg gcgaaaatag acaccagcac cagcgactgg
     9361 caggcgtaaa atccgcatga caggcgatag cttttgacac taatcacaaa cagcgatgtc
     9421 agcatcatca gtcccgccag attatttacg atcatagaac cggtcattca tcatctcctt
     9481 actgcaacca ggtcgcggcg attacgctgg aaatcagcga catcacaatc agtacaatca
     9541 acaccaggcg cattgatcct ggcagcggtg cggcatcggc gacggcctcg ctcggtttgc
     9601 caggcacgac gcgaccaaac cagcgaataa accaggcgaa actggcgacc gattcaatca
     9661 tcagcagaat catggcgggc agcaggatcc agtactccac tgacaacgaa aaaccggcag
     9721 caaacagcgg gaatttactg aagaagccgt tgaacggcgg tacgccagta atcgccagcg
     9781 cggctacgca gaaacccacg cctggcaacg gcagggtgtg caatacgcca cgcagacgcg
     9841 gcaacaagcg cgtgccgcag ctgtaactca gcgcacctgc tacaaggaaa aacaggcttt
     9901 tagcgaacgc gtggttgacg atgtaggcga tgctgccctc cagcgacagc cgcgagccga
     9961 agatggagag cgacaagccg aaaaacatcc agccaagttg agtgatggtc gaccaggcca
    10021 gcaaccgctt catatcctgc tgtggcaaat acatcaggaa gccgtaaaga atggtgacca
    10081 gtgccataac catgccaacg ccgccaatca catgcgggat attgccgccg tcgataatgg
    10141 cacgggcaaa aatgtaaacg cccactttca ccatcgatgc ggcgtggaga taggcgctga
    10201 tcggtgttgg cgcttccatt gcatccggta gccacgcttg catcggtagc tgggccgatt
    10261 tcccccacgc ggcaaacaga atgccgccat aaaccagata acgtgcgtcg ccgtgtaact
    10321 cgctcatcgc gctaagcgca aacgttccgg tctgcaaaaa cagcgtggcg gcggcaagat
    10381 acaaccccag cgaaccgata tgagtgataa gtaacgcttt tagtgctgaa cgctgcgctt
    10441 tatcgctctg gtaataactg atcaacgccc aggagcagcc gcccgtaatt tcaaaaaaca
    10501 acaactgacc gagcagcgtt gaggagagta ccagtcccgc catcgcgccg ataaacacca
    10561 gcaaaaatgc gtaataacga ttcgtgccgt tatgcgggtg ttcgcgattt ttatccgtca
    10621 gataacccgt ggagtagatc gtgaccagca aaccgaggaa caccaccaca aacagaatca
    10681 gcgtactcac gcgatcaatg accaggccaa acaacgccac atcccccacg ttgaccaacg
    10741 tcaacgtgac ggcaacttta tcggcctgat aaaacgcgga gatcagcgac aacatgcaca
    10801 gcgtggtcag cgcggcgaac aaaatccccc attcggcggc ccgacgttgt ggcgaaaacg
    10861 aaacgaccag tgcgccgata aaaggcagca ataacgtcgt cagagcaaga ttttccatat
    10921 tccgtcagtg ctccttacag accggttaac cagaagaccc aggcaagcgc agcaaggctg
    10981 aagccaagcc aggtcacatg gtgaatgagt aaaaaacgcc cgcgtgccag cgtgttttct
    11041 gcgatagagg ccagaacaaa aatcagcaaa accttgagca gcgtaacgac aagtgaagtc
    11101 agcaggcagg tgagagaaag ctcttgcgcg cgcccaaagg gcagaaacag ggccacaaac
    11161 agtgatgcca tcacgacctg tttcagcccc agccccaact tcgccagcgc cagcccggca
    11221 ccggaatatt cggtcagcgg gccttcctgt aattcctgtt ctgcttcagc aacatcaaag
    11281 ggaatttttc ccatctcaat gaagcaggca aaaccacagg ccagtaacgc cagtacggtg
    11341 gttagcggcg agttccagcc cgtcgccagc gtcttgctga tcatctcgat atgcgtggaa
    11401 tccgctatca gcgccaatac cagcagtgag agaatgagca tgggttcgac cagaatgccg
    11461 agcgttaact cgcgactggc accgattccc gcaaaggggc ttccggtatc cagcccggaa
    11521 agagcaaaga aaaaacgaaa tagggcaaga agatagataa gggtgatcag atcgccgccg
    11581 cccgcaaaag gggaaacagt aataaatagt ggtagggcca tcgccagcac cagcatgctg
    11641 ctgattaata cccacggcat caggcggaac atcagacctg aagatgtcgg cgcaacttcc
    11701 tggcgtttaa acagtttgtg gatatcgcga taatcctgcc agatccccgg tccgcgacgg
    11761 gagtgcatac gcgcgcgtat ctgccgggaa atccccgtaa aaagcggggt tagcgccagc
    11821 agaatcacgg cctgtgctaa cgcaaaaatg atcagatatc cgtcgcaaag agtttgtctc
    11881 atggtgattt ccttagacgg caatagcgat tagcagcaca accagtgcgg cgaccacata
    11941 caggcaatag agacgaaagt cgccgctttg cagatgctgt atttctttgg ccagccgttg
    12001 ggtggcgctc acgatggggc ggatcacccg ctcatcccag aagggttctg tgctctgagc
    12061 cctggcggtg acgtgcgcaa gacctttgtt cagcctcagc gtagggtcga gctgttgtcg
    12121 tagacgaaat agcgcacaaa agaccacacg cagcggctgc atcacgccat tgcctgacgg
    12181 ggccatcgca ttatcccagc cgtagccgca tgcccaggca tctcctgtgc gacgaaacgc
    12241 tgcgcggcgc gaacgacacc acagccagta aaggccaggc agcaaaggca tcgccagtag
    12301 taacagcaac agtaatgagg gagtgacccg tgtatgaaac gtgccgggta caagtgctat
    12361 tccgctggcg acagtgacgg gaggggtatt ggtaaacgca tgggcaatgt gcattatctt
    12421 cggtgccagc caactggcac taagcgcaat gaatacgcag agtgccgcga gcagtagcat
    12481 ggcgacgatc atcgtatttg gcacgtcctg tgcctcttca gcgtgtgtac tgcgcggcgc
    12541 accacagaaa gtaatgccgt acattttgac gaagcacatt actgccagcc caccagtgac
    12601 tgccagcatc accatagcaa taggacccgc aagttgtagc gctacggctt ccacacgact
    12661 tagtgagaac agcgactgcc aggtgtacca ttcgctgata aaaccattca gcggaggaat
    12721 ggctgatatc gcgaggcaac caatcaggca tgctgcggct gtccacggca tccgttttgc
    12781 cagtgccccc attttttcca tgtcgtgggt atgcaaacgc gaaataatcg cacccgcgcc
    12841 gagaaatagc agccctttga acagcgcatg gttgagcaga tgaaacagtg cgccgagcag
    12901 tccaaccatg gtgagcagcg ggtcgtgcag tgacagaccg accatggaca caccgaccgc
    12961 cagcaaaata atgccgacgt tttcgacggt actccaggcc agcagccgtt tgatatcctg
    13021 ttccgccagc gcatacagca cgcccaggag cgcggagatt gcgccgatcg ccatcaccag
    13081 aatgccccac cacagcggca aacccgtttg cgccagcaga tccatcgcca ctttcaggat
    13141 gccgaaaata ccgattttga ccattacgcc agacatcaac gccgaagcgt gcgatggtgc
    13201 ggcagggtga gcgcgcggca accagctgtg caacggcatc atcccggctt tcgcgccaaa
    13261 accgaagaag gccagcagga acaccgccga cgccagcccc ggagaaagtg aaagcgtgcg
    13321 gaaactggca aaatcgaggc tgccgctttc gcgccccatc agcaagaagg cgatcataat
    13381 cagcaccgat ccggcgtggg cgataaaaac gtagagcatg ccagcgttga ttgacgtttt
    13441 atcctgcctg gcaatgacca gaaaccagga agacagcgac atcatttcaa acagcacgat
    13501 gaaccaaaaa gcgttatcca tcaccagcag ggcaaccatc gaggcgatga aaagattcat
    13561 aaagaagccc atcgccgccg cgcctttgcc ctcgtattcg cgcatataag tcaatgaata
    13621 gagcgaacaa accaccacca gcaaggagat aaccagcacc ataaatgccg agaggctatc
    13681 cattcgcagg gttaaatcgg caaagtcaaa aggcgtggca aaacgggcga ctaaagttac
    13741 gcctgcatgt aattgcgtaa ttccgctgat gatgccaatc acgccgccca ccagcgatgt
    13801 gatgccggaa agcttaatag ccagtctgtc cagaccgagt aaaaacagcg aagccagact
    13861 ggcaaacaga tagaggatca gcgaccaggt taataattgc agggcatcca ttagcgttgc
    13921 tcctgagtga gggaaagggg atcctccccg ccattggcaa accagcttgc ggcaaggcgc
    13981 tgtttttctt tcatctgacg ttgcagtcta tcgtcggtga tcagccgtaa cgcctgattc
    14041 ggacaagcgc gaacgcaggc cggaccttct ggcaagaaat cacacaggtc gcatttcacc
    14101 gcgacggtct ggacacccgg ttcccagcgc agcaaagcat gttgggtcga cacgttttct
    14161 tcgccgtctt tgagtgagcc ctcagcctga aaaacatatt gcgcaggggc attcaccgga
    14221 cggcttcctg aagcgctgat tgcgccaaat gggcagacca cggcgcaaag cttgcaacca
    14281 atacaaaggc tttcgttgag ttggatcgca tcatccctct gggagatggc attgaccggg
    14341 cagacctgca ggcaaggggc ttcctcacag tgatgacaca cgacaggggc agtgagtgtt
    14401 gacgtcttcg ccagggccag gcgcgggtgt tgctgtaaac cttgcgtttt atgcacgtcc
    14461 gaacaggcag cgagacaggt attacatcct gtacaccaca gtggttcggc caccacaaag
    14521 cggttcatgt cggctccttg aaaatgatat gaaacattgc atttcatatt gcataaagaa
    14581 tgccagtctg attaatttaa tatattcaat aagttatgaa ttaactgtat tctgaacggt
    14641 acaaaaatga tggtcattaa cgttcagcaa taaatggatt cgacacccgt gtcgcagggg
    14701 aaagcgagaa tgtgatcttg atcacgagaa gtgaggtgtg ctggccgcaa gcacagccag
    14761 cacggaatgg agcaaagtaa tcaggcgtgt tctttcagcc agttcagcac aacgtcgtgg
    14821 tgattgctgg ttttgaaatc gtcaaagaca tgttcgattt tgccatcagc gtcaatcagg
    14881 aagctgatgc gatgaatacc atcgtaggtt ttgcccatga aggacttttc accccagacg
    14941 ccgaattgtt cgcacacctg gtggtcctca tcagacagga gcgtaaagtt aagcagctct
    15001 ttttccgcaa aacgggagag tttttcgggt ttatcggtgc tgatacccag cacatcgacg
    15061 cccgcttttt tcaactcatc catgttatcg cgtaagccgc aggcctgtac ggtacagccg
    15121 ggggtcatgg ctttcgggta gaaataaacc agaacacgct gtccctggaa gtcggtcaaa
    15181 ttaacttgtt ctccgtcttg atccggcaag ctaaatttcg gtgcgatatc accggctttc
    15241 agtggattca ttacttaact ccatcctgtt catcatgttg ggaataattg acgacgttaa
    15301 tactgccttg tgcattgagt tctgtacata gggctttgaa cgcttgctca atatttgctg
    15361 cgtccgcaga tgcggggctg tgggcagtta tctgaatatg caactgcgca gcccgttcat
    15421 tttcagcagg ttgcgtgcgc gacaccagct ccgcaatgtt catatgatgc gcgtcgaaaa
    15481 gtgctgtgaa gcgttcaatt aaatgcgggg agtctgccac atcgacctga acccagacag
    15541 atgctggcat tggcggacgc ggacgcgccg tcgtgcgctt catcacgatt aaaagatcca
    15601 gttcggcacc tttcaacggt aacgttgatt caatcagagt aatggcattc catgaaccgg
    15661 aaagcagcat gataaacgtg aactcttctc ccagcatcgc caggcgactg tcttcaatat
    15721 tacagccgca actactgaca tgacgggtga tggtgttcac tattccaggg cgatcggcac
    15781 ccaacgcagt gatcaccaga taatgttgcg atgacagtgt caaactggtt attccttcaa
    15841 ggggtgagtt gttcttaagg aaagcataaa aaaaacatgc atacaacaat cagaacggtt
    15901 ctgtctgctt gcttttaatg ccataccaaa cgtaccattg agacacttgt ttgcacagag
    15961 gatggcccat gttcacggga agtattgtcg cgattgttac tccgatggat gaaaaaggta
    16021 atgtctgtcg ggctagcttg aaaaaactga ttgattatca tgtcgccagc ggtacttcgg
    16081 cgatcgtttc tgttggcacc actggcgagt ccgctacctt aaatcatgac gaacatgctg
    16141 atgtggtgat gatgacgctg gagctggctg acgggcgcat tccggtgatt gccgggaccg
    16201 gtgctaacgc tactgcggaa gccattagcc tgacgcagcg cttcaatgac agcggtatcg
    16261 tcggctgcct gacggtaacc ccttactaca atcgtccgtc gcaagaaggt ttgtatcagc
    16321 atttcaaagc catcgctgaa catactgacc tgccgcaaat tctgtataat gtgccgtccc
    16381 gtactggctg cgatctgctc ccggaaacgg tgggccgtct ggcgaaagta aaaaatatta
    16441 tcggaatcaa agaggcaaca gggaacttaa cgcgtgtaaa ccagatcaaa gagctggttt
    16501 cagatgattt tgttctgctg agcggcgatg atgcgagcgc gctggacttc atgcaattag
    16561 gcggtcatgg ggttatttcc gttacggcta acgtcgcagc gcgtgatatg gcccagatgt
    16621 gcaaactggc agcagaaggg cattttgccg aggcacgcgt tattaatcag cgtctgatgc
    16681 cattacacaa caaactattt gtcgaaccca atccaatccc tgtgaaatgg gcatgtaagg
    16741 aactgggtct tgtggcgacc gatacgctgc gcctgccaat gacacctatc accgacagtg
    16801 gtcgtgagac ggtcagagcg gcgcttaagc atgccggttt gctgtaaagt ttagggagat
    16861 ttgatggctt actctgttca aaagtcgcgc ctggcaaagg ttgcgggtgt ttcgcttgtt
    16921 ttattactcg ctgcctgtag ttctgactca cgctataagc gtcaggtcag tggtgatgaa
    16981 gcctacctgg aagcggcacc gcttgcggag cttcatgccc cggctggaat gattttgccg
    17041 gtgacctccg gtgattatgc aatcccggtg accaacggta gtggtgctgt cggtaaggcg
    17101 ctggacattc gtccaccagc ccagccgctg gcactggttt ctggcgcgcg tacccagttc
    17161 acgggcgata ccgcttcatt gctggtggaa aatggtcgtg gcaatactct gtggccgcag
    17221 gtggttagcg tgctgcaggc gaaaaactac accatcaccc aacgtgatga tgctggtcag
    17281 acactgacca ccgattgggt acaatggaac cgtctggacg aagacgagca gtatcgtggt
    17341 cgttatcaaa tctctgttaa gccgcagggt tatcagcagg cggttacggt taaactgctg
    17401 aacctggaac aggcgggcaa accggttgca gacgcggctt ccatgcagcg ttacagcacg
    17461 gagatgatga acgttatttc cgccggtctg gataaatctg ccactgacgc cgcgaacgct
    17521 gcgcaaaatc gtgcctccac cactatggac gtacaaagtg cagctgatga caccggttta
    17581 ccgatgctgg tcgtacgcgg gccgttcaat gtggtttggc aacgtctgcc agcggcgctg
    17641 gaaaaagtgg gcatgaaagt gaccgacagc acccgttcgc agggtaacat ggccgtaact
    17701 tacaagccgc tgtctgacag cgactggcag gaactgggcg cgagcgatcc aggcctggca
    17761 tccggtgact ataaactgca ggttggcgat ttagataacc gcagcagcct acagttcatc
    17821 gatccgaaag gtcatactct gactcagagt cagaatgacg cgctggtagc tgtcttccag
    17881 gctgcgttta gcaagtaaaa atacagggct ggagtcatcc ggcccttttt tctgatatga
    17941 tacgcaaacg tgtgcgtcgg cagaaaaacg cgattttagc ggtaattcgc acgaaatttg
    18001 tttgtcggac gtaggtcgga taaggcgttc acgccgcatc cgacaaaaca tctggcacac
    18061 cagacagcaa tagattttaa aacgttaatt cacacccagg agtgataaag atgcaaaagc
    18121 aagctgagtt gtatcgtggt aaagcgaaaa ccgtatacag cacggaaaac ccggacctgt
    18181 tggtgctcga attccgcaat gatacgtcag caggggatgg cgcgcgcatt gagcagtttg
    18241 atcgcaaagg tatggtgaac aacaagttca actacttcat tatgagcaaa ctggctgaag
    18301 cgggtatccc gactcaaatg gagcgtctgc tctccgatac cgaatgtctg gtgaaaaagc
    18361 tggatatggt gccggttgag tgtgtcgtgc gtaaccgtgc tgctggctct ctggtgaaac
    18421 gtcttggaat cgaagaaggt attgagctga acccgccact gttcgatctg ttcctgaaaa
    18481 acgacgccat gcacgatccg atggtcaacg aatcttactg cgaaaccttt ggctgggtga
    18541 gcaaagagaa cctggcgcgt atgaaagagc tgacctacaa agcgaacgac gtgctgaaaa
    18601 aactgttcga tgatgctggt ctcattctgg tcgacttcaa gctggagttt ggtctgtaca
    18661 aaggcgaagt ggttctgggc gatgagttct ccccggacgg cagccgcctg tgggacaaag
    18721 aaacgctgga gaaaatggac aaagaccgtt tccgccagag cctcggtggc ctgatcgaag
    18781 cctatgaagc cgttgcccgc cgcctgggtg tacagctgga ctgatttttc tattcatcat
    18841 cttgccgtgc tgctggcacg gcaagacaac cgctctcata agatgtgcat tgaacgctca
    18901 ttcctccgcc atttcatccc gctttcctgt ggtaatcttc ctctgaacca cctacgatca
    18961 tttctataat gaatatattg ttgaggtatc tatgcgttgg caagggcgac gtgaaagtga
    19021 caatgttgaa gacaggcgca atagctccgg cggtccgtca atgggcggtc ccggttttcg
    19081 cctgccaagc ggtaaaggcg ggctgatttt actgatagtc gtgctggttg caggctacta
    19141 tggcgttgat ttaaccgggt tgatgaccgg acagccggtt tcccaacagc aatcaacgcg
    19201 gtcaattagc cctaatgaag acgaagccgc aaaattcacc tcggtgattc tggcaaccac
    19261 ggaagacacc tggggacagc aattcgagaa gatgggtaaa acctatcagc aaccgaagct
    19321 ggtcatgtac cgtggaatga cgcgtaccgg ctgcggggcg ggacagtcca taatggggcc
    19381 attctattgc ccggcggatg gcacagttta tatcgatctc tccttctatg atgacatgaa
    19441 agacaaactt ggcgcggatg gcgattttgc ccaggggtac gttatcgctc atgaagtcgg
    19501 tcatcatgtg cagaaactgc taggcatcga gccgaaagtt cgtcaactgc aacaaaacgc
    19561 gacgcaggcg gaagtaaacc gcttatctgt gcgtatggaa ctccaggccg actgttttgc
    19621 cggtgtctgg gggcatagta tgcagcagca aggtgttctg gaaaccggcg atctggaaga
    19681 ggcgctgaac gcggcgcagg ccatcggcga tgaccgttta caacagcaaa gtcaggggcg
    19741 agtagtacca gacagtttca ctcatggcac ttctcagcaa cgctacagct ggtttaaacg
    19801 tggtttcgac agcggcgatc cggcacaatg caatactttt ggtaaaagca tttaacttcc
    19861 ggggcaggga tggctgaact gactgcgctt cacacattaa cagcgcaaat gaaacgtgaa
    19921 gggatccgcc gcttgctggt gttgagcggg gaagagggtt ggtgttttga tcatgcgctt
    19981 aagttgcgtg atgccttacc tggcgactgg ctgtggattt cgccgcagcc agatgctgaa
    20041 aaccactgtt ctccctcggc actacaaact ttacttgggc gcgagttccg gcatgcggta
    20101 ttcgacgccc gccacggctt tgatgccgct gcctttgcgg cacttagcgg aacgttgaaa
    20161 gcgggaagct ggctagtgtt gttactccct gtatgggatg agtgggaaaa ccaacctgat
    20221 gccgactcgc tgcgctggag tgattgccct gaccctattg cgacgccgca ttttgtccag
    20281 catttcaaac gcgtacttac ggcgaataac gacgctatcc tctggcggca aaaccagccg
    20341 ttctcgttgg cgcattttac tccccgtact gactggcacc ccgcgaccgg cgcaccacag
    20401 ccagaacaac agcaactctt acagcagcta ctgaccatgc cattgggcgt ggcggtggta
    20461 acggctgcgc gtgggcgcgg taaatcggcg ctggcagggc aactcatttc tcgtattgcg
    20521 ggtagtgcga ttgtcaccgc gcccgcaaaa gcggcaacgt atgtactggc acaatttgcg
    20581 ggcgagaagt ttcgctttat tgcaccggat gccttgttag ccagcgatga gcaagccgac
    20641 tggctggtgg tcgatgaagc cgcagccata cctgcgccgt tgttgcatca actggtatcg
    20701 cgttttcctc gaacgttgtt aaccactacg gtgcagggct acgaaggcac cggacgtggt
    20761 tttttgctga aattttgcgc tcgctttccg catttacacc gttttgaact gcaacaaccg
    20821 atccgctggg cgcagggatg cccgctggag aaaatggtca gcgaggcact ggtgtttgac
    20881 gatgaaaact tcactcacga accacaaggt gacatcgtca tttctgcttt tgaacagacg
    20941 ttatggcgaa gcgaaccaga aacgccgtta aaggtatatc agctattgtc tggcgcgcac
    21001 taccggacct cgccgctgga tttacgccgg atgatggatg caccagggca acatttttta
    21061 caggcggctg gcgaaaacga gattgccgga gcgctgtggc tggtggatga ggggggatta
    21121 tctcaacaac tcagtcaggc ggtatgggca ggttttcgtc gccctcgggg taatctggtg
    21181 gcccagtcgc tggcggcgca cggcagcaat ccactggcag cgacattgcg tggacggcgg
    21241 gtaagccgga tagcagttca tccggcgcgt cagcgggaag gcacagggcg gcaacttatt
    21301 gctggtgctt tgcaatatac gcatgacctc gactatcttt cggtgagttt tggttacacc
    21361 ggggagttat ggcgtttctg gcaacg
//
LOCUS       DAMZFT010000082        21232 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.082, whole genome shotgun sequence.
ACCESSION   DAMZFT010000082 DAMZFT010000000
VERSION     DAMZFT010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21232)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21232)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21232
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.082"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..1066
                     /gene="lolE"
                     /locus_tag="PN977_003181"
     CDS             <1..1066
                     /gene="lolE"
                     /locus_tag="PN977_003181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolE"
                     /protein_id="HDI6043191.1"
                     /translation="AVVPHGEIEAVDQPWTNWQEALDNVQKVPGIAAAAPYINFTGLV
                     ESGANLRAIQVKGVNPQQEQRLSALPSFVQGDAWRNFKAGEQQIIIGKGVADALKVKQ
                     GDWVSIMIPNSNPEHKLMQPKRVRLHVAGILQLSGQLDHSFAMIPLADAQQYLDMGSS
                     VSGIALKMTDVFNANKLVRDAGEVTNSYVYIKSWIGTYGYMYRDIQMIRAIMYLAMVL
                     VIGVACFNIVSTLVMAVKDKSGDIAVLRTLGAKDGLIRAIFVWYGLLAGLFGSLCGVI
                     IGVVVSLQLTPIIEWIEKLIGHQFLSSDIYFIDFLPSELHWLDVFYVLVTALLLSLLA
                     SWYPARRASNIDPARVLSGQ"
     gene            1095..2006
                     /gene="nagK"
                     /locus_tag="PN977_003182"
     CDS             1095..2006
                     /gene="nagK"
                     /locus_tag="PN977_003182"
                     /EC_number="2.7.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine kinase"
                     /protein_id="HDI6043192.1"
                     /translation="MYYGFDIGGTKIALGVFDSGRQLQWEKRVPTPRDSYDAFLDAVC
                     ELVAEADQRFGCKGSVGIGIPGMPETEDGTLYAANVPAASGKPLRADLSARLDRDVRL
                     DNDANCFALSEAWDDEFTQYPLVMGLILGTGVGGGLIFNGKPITGKSYITGEFGHMRL
                     PVDALTMMGLDFPLRRCGCGQHGCIENYLSGRGFAWLYQHYYHQPLQAPEIIALYDQG
                     DEQARAHVERYLDLLAVCLGNILTIVDPDLVVIGGGLSNFPAITTQLADRLPRHLLPV
                     ARVPRIERARHGDAGGMRGAAFLHLTD"
     gene            2022..2843
                     /gene="cobB"
                     /locus_tag="PN977_003183"
     CDS             2022..2843
                     /gene="cobB"
                     /locus_tag="PN977_003183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HDI6043193.1"
                     /translation="MLSRRGHRLSRFRKNKRRLRERLRQRIFFRDKVVPEAMEKPRVL
                     VLTGAGISAESGIRTFRAADGLWEEHRVEDVATPEGFDRDPELVQAFYNARRRQLQQP
                     EIQPNAAHLALAKLQDALGDRFLLVTQNIDNLHERAGNTNVIHMHGELLKVRCSQSGQ
                     VLDWTGDVTPEDKCHCCQFPAPLRPHVVWFGEMPLGMDEIYMALSMADIFIAIGTSGH
                     VYPAAGFVHEAKLHGAHTVELNLEPSQVGNEFAEKYYGPASQVVPEFVEKLLKGL"
     gene            complement(2981..3769)
                     /locus_tag="PN977_003184"
     CDS             complement(2981..3769)
                     /locus_tag="PN977_003184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HDI6043194.1"
                     /translation="MKKIMILLSLLMFLPLTAISKPLIPIMKTLFTDVTGTVPDAEEI
                     AHKAELFRQQTGVVPFIVVLPDINNEASLRQNGKAMLAHAASSMSNVKGSILLLFTTR
                     EPRLIMITNGQVESSMDDKHLGLLVENHTLAYLHADLWYQGINNALAVLQAQILKQPT
                     PPLTYYPHPGQQHENDPPGSTTTLGLFAWAVAFIVFAAFFNYTTRLYYALKFAVAMAV
                     ANMGYQALCLYIDNSFAITRISPLWAGLIGVCTFIAALLWTSKR"
     gene            complement(3766..4227)
                     /locus_tag="PN977_003185"
     CDS             complement(3766..4227)
                     /locus_tag="PN977_003185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3592 domain-containing protein"
                     /protein_id="HDI6043195.1"
                     /translation="MSQDSKVFFRIFFGIGLVLILISVVIFYNQFTYSKDAIHTEGVI
                     VDTVWHSSHSHRTGKNGSWYPVVAFRPTPDYTLIFNSSIGSDFYEDSEGDKVNVYYSP
                     GHPEKAEINNPWVNFFKWGFIGIMGVIFIAVGLLISMPSSKKTRRKRKSRP"
     gene            complement(4285..5331)
                     /gene="potD"
                     /locus_tag="PN977_003186"
     CDS             complement(4285..5331)
                     /gene="potD"
                     /locus_tag="PN977_003186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotD"
                     /protein_id="HDI6043196.1"
                     /translation="MKKWSRHLLAAGALALGMSAAHADDNNTLYFYNWTEYVPPGLLE
                     QFTKETGIKVIYSTYESNETMYAKLKTYKDGAYDLVVPSTYYVDKMRKEGMIQKIDKS
                     KLSNFSNLDPDMLNKPFDPNNDYSIPYIWGATAIGVNGDAVDPKSVTSWADLWKPEYK
                     GSLLLTDDAREVFQMALRKLGYSGNTTDPKEIEAAYNELKKLMPNVAAFNSDNPANPY
                     MEGEVNLGMIWNGSAFVARQAGTPIDVVWPKEGGIFWMDSLAIPANAKNKEGALKLIN
                     FLLRPDVAKQVAETIGYPTPNLAARKLLSPEVANDKTLYPDAETIKNGEWQNDVGAAS
                     SIYEEYYQKLKAGR"
     gene            complement(5328..6122)
                     /gene="potC"
                     /locus_tag="PN977_003187"
     CDS             complement(5328..6122)
                     /gene="potC"
                     /locus_tag="PN977_003187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotC"
                     /protein_id="HDI6043197.1"
                     /translation="MIGRLLRGGFMTAIYAYLYIPIIILIVNSFNSSRFGINWQGFTT
                     KWYSLLMNNDSLLQAAQHSLTMAVFSATFATLIGSLTAVALYRYRFRGKPFVSGMLFV
                     VMMSPDIVMAISLLVLFMLLGIQLGFWSLLFSHITFCLPFVVVTVYSRLKGFDVRMLE
                     AAKDLGASEFTILRKIILPLAMPAVAAGWVLSFTLSMDDVVVSSFVTGPSYEILPLKI
                     YSMVKVGVSPEVNALATILLVLSLVMVIASQLIARDKTKGNTGDVK"
     gene            complement(6119..6976)
                     /gene="potB"
                     /locus_tag="PN977_003188"
     CDS             complement(6119..6976)
                     /gene="potB"
                     /locus_tag="PN977_003188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000799378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotB"
                     /protein_id="HDI6043198.1"
                     /translation="MKNTSKFQNVVIVTIVGWLVLFVFLPNLMIIGTSFLTRDDASFV
                     KMVFTLDNYTRLLDPLYFEVLLHSLNMALIATLACLVLGYPFAWFLAKLPHKVRPLLL
                     FLLIVPFWTNSLIRIYGLKIFLSTKGYLNEFLLWLGVIDTPIRIMFTPSAVIIGLVYI
                     LLPFMVMPLYSSIEKLDKPLLEAARDLGASKLQTFIRIIIPLTMPGIIAGCLLVMLPA
                     MGLFYVSDLMGGAKNLLIGNVIKVQFLNIRDWPFGAATSITLTIVMGLMLLVYWRASR
                     LLNKKVELE"
     gene            complement(6960..8096)
                     /gene="potA"
                     /locus_tag="PN977_003189"
     CDS             complement(6960..8096)
                     /gene="potA"
                     /locus_tag="PN977_003189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     ATP-binding protein PotA"
                     /protein_id="HDI6043199.1"
                     /translation="MGQSKKLNKQPSSLSPLVQLAGIRKCFDGKEVIPQLDLTINNGE
                     FLTLLGPSGCGKTTVLRLIAGLETVDSGRIMLDNEDITHVPAENRYVNTVFQSYALFP
                     HMTVFENVAFGLRMQKTPAAEITPRVMEALRMVQLETFAQRKPHQLSGGQQQRVAIAR
                     AVVNKPRLLLLDESLSALDYKLRKQMQNELKALQRKLGITFVFVTHDQEEALTMSDRI
                     VVMRDGRIEQDGTPREIYEEPKNLFVAGFIGEINMFNATVIERLDEQRVRANVEGREC
                     NIYVNFAVEPGQKLHVLLRPEDLRVEEINDDNHAEGLIGYVRERNYKGMTLESVVELE
                     NGKMVMVSEFFNEDDPDFDHSLDQKMAINWVESWEVVLADEEHK"
     gene            8346..9572
                     /gene="pepT"
                     /locus_tag="PN977_003190"
     CDS             8346..9572
                     /gene="pepT"
                     /locus_tag="PN977_003190"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HDI6043200.1"
                     /translation="MDKLLERFLNYVSLDTQSKAGVRQVPSTEGQWKLLHLLKEQLEE
                     MGLINVTLSEKGTLMATLPANVPGDIPAIGFISHVDTSPDCSGKNVNPQIVENYRGGD
                     IALGIGDEVLSPVMFPVLHQLLGQTLITTDGKTLLGADDKAGIAEIMTALAVLQQKNI
                     PHGDIRVAFTPDEEVGKGAKHFDVDAFDARWAYTVDGGGVGELEFENFNAASVNIKIV
                     GNNVHPGTAKGVMVNALSLAARIHAEVPADESPEMTEGYEGFYHLASMKGTVERADMH
                     YIIRDFDRKQFEARKRKMMEIAKKVGKGLHPDCYIELVIEDSYYNMREKVVEHPHILD
                     IAQQAMRDCDIEPELKPIRGGTDGAQLSFMGLPCPNLFTGGYNYHGKHEFVTLEGMEK
                     AVQVIVRIAELTAQRK"
     gene            complement(9621..10742)
                     /gene="roxA"
                     /locus_tag="PN977_003191"
                     /gene_synonym="ycfD"
     CDS             complement(9621..10742)
                     /gene="roxA"
                     /locus_tag="PN977_003191"
                     /gene_synonym="ycfD"
                     /EC_number="1.14.11.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309600.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[50S ribosomal protein L16]-arginine
                     3-hydroxylase"
                     /protein_id="HDI6043201.1"
                     /translation="MEYQLTLNWPDFLERHWQKRPVVLKRGFNNFIDPISPDELAGLA
                     MESEVDSRLVSHQDGKWQVSHGPFESYDHLGETNWSLLVQAVNHWHEPTAALMRPFRE
                     LPDWRIDDLMISFSVPGGGVGPHLDQYDVFIIQGTGRRRWRVGEKLQMKQHCPHPDLL
                     QVDPFEAIIDEELEPGDILYIPPGFPHEGYALENAMNYSVGFRAPNTRELISGFADYV
                     LQRELGGNYYSDPDVPPRAHPADVLPQEMDKLREMMLELINQPEHFKQWFGEFISQSR
                     HELDIAPPEPPYQPDEIYDALKQGDVLVRLGGLRVLRIGDDVYANGEKIDSPHRPALD
                     ALASNIALTAENFGDALEDPSFLAMLAALVNSGYWFFEG"
     gene            complement(10818..12278)
                     /gene="phoQ"
                     /locus_tag="PN977_003192"
     CDS             complement(10818..12278)
                     /gene="phoQ"
                     /locus_tag="PN977_003192"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PhoQ"
                     /protein_id="HDI6043202.1"
                     /translation="MKKLLRLFFPLSLRVRFLLATAAVVLVLSLAYGMVALIGYSVSF
                     DKTTFRLLRGESNLFYTLAKWENNKLHVELPENIDKQSPTMTLIYDENGQLLWAQRDV
                     PWLMKMIQPDWLKSNGFHEIEADVNDTSLLLSGDHSIQQQLQEVREDDDDAEMTHSVA
                     VNVYPATSRMPKLTIVVVDTIPVELKSSYMVWSWFIYVLSANLLLVIPLLWVAAWWSL
                     RPIEALAKEVRELEEHNRELLNPATTRELTSLVRNLNRLLKSERERYDKYRTTLTDLT
                     HSLKTPLAVLQSTLRSLRSEKMSVSDAEPVMLEQISRISQQIGYYLHRASMRGGTLLS
                     RELHPVAPLLDKLTSALNKVYQRKGVNISLDISPEISFVGEQNDFVEVMGNVLDNACK
                     YCLEFVEISARQTDEHLYIVVEDDGPGIPLSKREVIFDRGQRVDTLRPGQGVGLAVAR
                     EITEQYEGKIVAGESMLGGARMEVIFGRQHSAPKDE"
     gene            complement(12278..12949)
                     /gene="phoP"
                     /locus_tag="PN977_003193"
     CDS             complement(12278..12949)
                     /gene="phoP"
                     /locus_tag="PN977_003193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator PhoP"
                     /protein_id="HDI6043203.1"
                     /translation="MRVLVVEDNALLRHHLKVQIQDAGHQVDDAEDAKEADYYLNEHL
                     PDIAIVDLGLPDEDGLSLIRRWRSNDVSLPILVLTARESWQDKVEVLSAGADDYVTKP
                     FHIEEVMARMQALMRRNSGLASQVISLPPFQVDLSRRELSINDEVIKLTAFEYTIMET
                     LIRNNGKVVSKDSLMLQLYPDAELRESHTIDVLMGRLRKKIQAQYPQEVITTVRGQGY
                     LFELR"
     gene            complement(13118..14488)
                     /gene="purB"
                     /locus_tag="PN977_003194"
     CDS             complement(13118..14488)
                     /gene="purB"
                     /locus_tag="PN977_003194"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002950897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HDI6043204.1"
                     /translation="MELSSLTAVSPVDGRYGDKVSALRGIFSEYGLLKFRVQVEVRWL
                     QKLAAHAAIKEVPAFAADAIGYLDAIVASFSEEDAARIKTIERTTNHDVKAVEYFLKE
                     KVAEIPELHAVSEFIHFACTSEDINNLSHALMLKTARDEVILPYWRQLIDGIKDLAAQ
                     YRDIPLLSRTHGQPATPSTIGKEMANVAYRMERQYRQLNQVEILGKINGAVGNYNAHI
                     AAYPEVDWHQFSEEFVTSLGIQWNPYTTQIEPHDYIAELFDCVARFNTILIDFDRDVW
                     GYIALNHFKQKTIAGEIGSSTMPHKVNPIDFENSEGNLGLSNAVLQHLASKLPVSRWQ
                     RDLTDSTVLRNLGVGIGYALIAYQSTLKGVSKLEVNRGHLLDELDHNWEVLAEPIQTV
                     MRRYGIEKPYEKLKELTRGKRVDAEGMKQFIDGLALPEEEKARLKAMTPANYIGRAIT
                     MVDELK"
     gene            complement(14492..15133)
                     /gene="hflD"
                     /locus_tag="PN977_003195"
     CDS             complement(14492..15133)
                     /gene="hflD"
                     /locus_tag="PN977_003195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high frequency lysogenization protein HflD"
                     /protein_id="HDI6043205.1"
                     /translation="MAKNYYDITLALAGICQSARLVQQLAHQGHCDGDALHVSLNSII
                     DMNPSSTLAVFGGSEANLRVGLETLLGVLNASSRQGLNAELTRYTLSLMVLERKLSSA
                     KGALDTLGNRINGLQRQLEHFDLQSETLMSAMAAIYVDVISPLGPRIQVTGSPAVLQS
                     PQVQAKVRATLLAGIRAAVLWHQVGGGRLQLMFSRNRLTTQAKQILAHLTPEL"
     gene            complement(15169..16275)
                     /gene="mnmA"
                     /locus_tag="PN977_003196"
     CDS             complement(15169..16275)
                     /gene="mnmA"
                     /locus_tag="PN977_003196"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309632.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HDI6043206.1"
                     /translation="MSETAKKVIVGMSGGVDSSVSAWLLQQQGYQVEGLFMKNWEEDD
                     GEEYCTAAADLADAQAVCDKLGIELHTVNFAAEYWDNVFELFLAEYKAGRTPNPDILC
                     NKEIKFKAFLEFAAEDLGADYIATGHYVRRADVDGKSRLLRGLDSNKDQSYFLYTLSH
                     EQIAQSLFPVGELEKPQVRKIAEDLGLVTAKKKDSTGICFIGERKFREFLGRYLPAQP
                     GKIITVDGDEIGEHQGLMYHTLGQRKGLGIGGTKEGTEEPWYVVDKDVENNILIVAQG
                     HEHPRLMSVGLIAQQLHWVDREPFTGTMRCTVKTRYRQTDIPCTVKALDDDRIEVIFD
                     EPVAAVTPGQSAVFYNGEVCLGGGIIEQRLPLPV"
     gene            complement(16329..16790)
                     /gene="nudJ"
                     /locus_tag="PN977_003197"
     CDS             complement(16329..16790)
                     /gene="nudJ"
                     /locus_tag="PN977_003197"
                     /EC_number="3.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase NudJ"
                     /protein_id="HDI6043207.1"
                     /translation="MFKPHVTVACVVHAEGKFLVVEETINGKALWNQPAGHLEADETL
                     VEAAARELWEETGISAQPQHFIRMHQWIAPDKTPFLRFLFAIELEQICPTQPHDSDID
                     CCRWVSAEEILQASNLRSPLVAESIRCYQSGQRYPLEMIGDFNWPFTKGVI"
     gene            complement(16800..17453)
                     /gene="rluE"
                     /locus_tag="PN977_003198"
     CDS             complement(16800..17453)
                     /gene="rluE"
                     /locus_tag="PN977_003198"
                     /EC_number="5.4.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415653.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2457) synthase RluE"
                     /protein_id="HDI6043208.1"
                     /translation="MRQFIISENTMQKTSFRNHQVKRFSSQRSTRRKPENQPTRVILF
                     NKPYDVLPQFTDEAGRKTLKEFIPVQGVYAAGRLDRDSEGLLVLTNNGALQARLTQPG
                     KRTGKIYYVQVEGIPTQDALEALRNGVTLNDGPTLPAGVDLVEEPEWLWPRNPPIRER
                     KSIPTSWLKITLYEGRNRQVRRMTAHVGFPTLRLIRYAMGDYSLDNLANGEWREATD"
     gene            17625..18875
                     /gene="icd"
                     /locus_tag="PN977_003199"
     CDS             17625..18875
                     /gene="icd"
                     /locus_tag="PN977_003199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HDI6043209.1"
                     /translation="MESKVVVPAQGKKITLQNGKLNVPENPIIPYIEGDGIGVDVTPA
                     MLKVVDAAVEKAYKGERKISWMEIYTGEKSTQVYGQDVWLPAETLDLIREYRVAIKGP
                     LTTPVGGGIRSLNVALRQELDLYICLRPVRYYQGTPSPVKHPELTDMVIFRENSEDIY
                     AGIEWKADSADAEKVIKFLREEMGVKKIRFPEHCGIGIKPCSEEGTKRLVRAAIEYAI
                     ANDRDSVTLVHKGNIMKFTEGAFKDWGYQLAREEFGGELIDGGPWLKVKNPNTGKEIV
                     IKDVIADAFLQQILLRPAEYDVIACMNLNGDYISDALAAQVGGIGIAPGANIGDECAL
                     FEATHGTAPKYAGQDKVNPGSIILSAEMMLRHMGWTEAADLIVKGMEGAINAKTVTYD
                     FERLMEGAKLLKCSEFGDAIIKNM"
     gene            complement(18978..19301)
                     /gene="iraM"
                     /locus_tag="PN977_003200"
     CDS             complement(18978..19301)
                     /gene="iraM"
                     /locus_tag="PN977_003200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraM"
                     /protein_id="HDI6043210.1"
                     /translation="MKWIVIDTVIQPTCGISFSAIWGDMKMIIWYQSTIFLPPGRIFT
                     PVKSGIILKDKEYPITIYNIASFNKDLWSLLKSSQECPPGESKITNKCLHNSCIIKIC
                     PYGLK"
     gene            complement(19560..19604)
                     /locus_tag="PN977_003201"
     CDS             complement(19560..19604)
                     /locus_tag="PN977_003201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6043211.1"
                     /translation="MFSKLAQSSIKAMF"
     gene            complement(19841..19951)
                     /locus_tag="PN977_003202"
     CDS             complement(19841..19951)
                     /locus_tag="PN977_003202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6043212.1"
                     /translation="MEIKVQRLFIWMSNTIFLLPPINNHQINTINLIFEM"
     gene            complement(20004..20408)
                     /locus_tag="PN977_003203"
     CDS             complement(20004..20408)
                     /locus_tag="PN977_003203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1398 domain-containing protein"
                     /protein_id="HDI6043213.1"
                     /translation="MDQVVVFQKMFEQVRKEQNFSWFYSELKHHRIAHYIYYLATDNI
                     RIITHDDTVLLLRGTRNLLKVSTTKNPAKIKEAALLHICGKSTFREYCSTLADAGVFR
                     WVTDVNHNKRSYYAIDNTLLYIEDVENNKPLI"
     gene            complement(20629..>21232)
                     /gene="bluR"
                     /locus_tag="PN977_003204"
     CDS             complement(20629..>21232)
                     /gene="bluR"
                     /locus_tag="PN977_003204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor BluR"
                     /protein_id="HDI6043214.1"
                     /translation="EEDIQRIEEIKRWISNGVPVGKVKALLETTSQDTEDDWSRLQEE
                     MMSILRMANPAKLRARIISLGREYPVDQLINHVYLPVRQRLVLDHNTSRIMSSMFDGA
                     LIEYAAASLFEMRRKPGKEAILMAWNVEERARLWLEAWRLSLSGWHISVLADPIESPR
                     PELFPTQTLIVWTGMAPTRRQNELLQHWGEQGYKVIFHAP"
BASE COUNT         5358 a         5139 c         5313 g         5422 t
ORIGIN      
        1 ggcggtggtg ccgcatggtg aaatcgaagc ggtggatcag ccgtggacta actggcagga
       61 agcactggat aacgtgcaga aagtgccggg tattgccgcc gctgcgccgt atatcaattt
      121 caccgggctg gtggaaagtg gcgcgaatct gcgcgcaatc caggtgaagg gcgttaaccc
      181 gcaacaggaa cagcgtctga gcgcattacc ctcgtttgtt cagggggatg cctggcgcaa
      241 ttttaaagcg ggcgaacagc aaattatcat cggcaaaggc gtggcggatg cgctgaaagt
      301 gaagcagggc gattgggtgt cgattatgat ccccaactcg aatcctgagc ataaactgat
      361 gcagccaaaa cgtgtgcgtt tgcacgttgc cggtattttg cagttgagtg gtcaactcga
      421 tcacagtttt gccatgatcc cgctggcgga tgcccaacaa tatcttgata tgggttccag
      481 cgtgtcaggt attgccctta aaatgacgga tgttttcaac gccaataagc tggtacgcga
      541 tgcgggtgaa gtgaccaaca gctatgttta tattaaaagc tggattggta cttacggcta
      601 tatgtatcgc gatatccaga tgatccgcgc cattatgtat ctggcgatgg tactggtgat
      661 tggcgtggcc tgtttcaaca tcgtctccac cttagtgatg gcggtgaaag acaagagtgg
      721 cgatatcgca gtattaagaa cgctgggggc gaaagatggt ttaattcgcg ccatctttgt
      781 ctggtatgga ttgctggcag ggctgttcgg cagcctgtgt ggggtgatta tcggcgtagt
      841 ggtttcactg caacttaccc cgattattga gtggattgaa aagctgatcg gtcatcagtt
      901 cctctccagc gatatctatt ttattgactt cttgccatcg gaattgcact ggctggacgt
      961 cttctacgta ctggtcacag cattgttgct gagtcttttg gcaagttggt atccggcgcg
     1021 gcgcgccagt aatattgacc ctgcgcgagt ccttagcggc cagtaaaggc agtacattaa
     1081 aacaaggagc ggcaatgtat tacgggtttg atattggtgg aacaaaaatt gcgctaggcg
     1141 tgtttgatag cggtcggcag ttgcagtggg aaaagcgggt gccgacaccg cgtgacagct
     1201 atgacgcatt tttagatgca gtgtgtgagc tggtagctga agctgaccaa cgttttggct
     1261 gtaaaggctc tgtcggcatc ggtattccgg gaatgccgga aacagaagat ggtacgctgt
     1321 atgccgccaa tgtccccgct gccagcggta aaccgctgcg tgccgacctg agcgcacgtc
     1381 ttgatcgcga tgtacgcctt gataacgatg ccaactgttt tgccctttca gaagcctggg
     1441 atgatgaatt tactcaatat ccactggtga tggggttgat tctcggcacc ggcgttggcg
     1501 gcgggctgat tttcaacggt aaaccaatta ccggtaaaag ctatattacc ggcgaatttg
     1561 gccatatgcg tctgccggtt gatgcgttaa ccatgatggg gctggatttc ccgttacgcc
     1621 gctgcggctg tggtcagcat ggctgcattg aaaattatct gtctggtcgc ggttttgcgt
     1681 ggctgtatca acactattat catcaaccgt tgcaggctcc tgagatcatt gcgctttatg
     1741 atcaaggcga tgagcaggca agggcgcacg ttgagcgtta tctggattta ttagcggttt
     1801 gtctgggaaa tatcctgacc attgttgacc ctgacctggt cgtcattggt ggaggcttat
     1861 cgaatttccc ggcaatcaca acgcaactgg cggacaggct gcctcgtcat ctcttacctg
     1921 tagctcgtgt tccgcgcatt gaacgcgcgc gccacggtga tgcgggggga atgcgtggtg
     1981 cggccttcct acatctaacc gattaatcaa cagaggttgc tatgctgtcg cgtcggggtc
     2041 atcggttaag tcgttttcgt aaaaataaac gccgcctgcg cgagcgtttg cgtcagcgta
     2101 tttttttcag agataaagtg gtgccggaag caatggaaaa accaagagta ctcgtactga
     2161 caggggcagg gatttctgcg gaatcaggta ttcgtacctt tcgcgccgca gatggcctgt
     2221 gggaagaaca tcgggttgaa gatgtggcaa cgccggaagg ttttgatcgc gatcctgaac
     2281 tggtgcaagc gttttataac gcccgtcgcc gacagctgca gcagccagaa attcagccta
     2341 acgccgcgca tcttgcgctg gctaaactgc aagacgccct cggcgatcgc tttttgctgg
     2401 tgacgcagaa tatcgacaac cttcatgagc gtgccggtaa caccaatgtt attcatatgc
     2461 atggggaact gctgaaagtt cgttgttcac aaagtggtca ggttctcgac tggaccggag
     2521 acgttacccc agaagataaa tgccattgct gccagttccc ggcacccttg cgaccgcacg
     2581 tagtgtggtt tggcgaaatg ccactcggca tggatgaaat ttatatggcg ttgtcgatgg
     2641 ccgatatttt cattgccatt ggtacttcgg ggcatgttta tccagcggct gggtttgttc
     2701 acgaagcgaa actgcatggc gcgcacaccg tggaactgaa tcttgaacca agtcaggtcg
     2761 gtaatgaatt tgccgagaaa tattacggcc cggcaagcca ggtggtgcca gaatttgttg
     2821 aaaagttgct gaagggatta taaagcggga aaaattgcct gatgcgctac gcttgtcagg
     2881 cctgtgaagg ggcgcaatta attgaatttg caggttttgt gggccggata aggcgttcgc
     2941 gccgcatccg gcataataca tgcgaacttt actgcctctt ttaccgttta ctcgtccata
     3001 gtaacgcggc aataaacgta cagacaccaa tcagccctgc ccagagtggc gatattctgg
     3061 taatcgcaaa gctgttatcg atatacaaac ataaagcctg ataacccata ttggcaacag
     3121 ccatcgccac tgcaaatttt aacgcataat aaaggcgcgt tgtgtagtta aaaaatgcag
     3181 caaagacgat gaatgcaact gcccaggcaa aaaggcctaa tgtcgtggta ctgccgggtg
     3241 gatcattttc atgctgttgt ccgggatgcg gataatatgt cagtggcggc gtcggttgtt
     3301 ttaatatctg tgcttgtaaa acagccaatg cattattgat tccctgatac cagagatctg
     3361 catgtaaata agccagagtg tgattttcta ccagaaggcc gagatgttta tcgtccatac
     3421 tgctttcgac ctggccgttg gttatcataa ttaaccgtgg ctcgcgggta gtaaagagta
     3481 acagaatact tccttttaca ttactcattg aagatgccgc atgcgccagc atcgctttgc
     3541 cattttgcct gagactggct tcattattaa tgtcgggtaa aaccacaata aacggtacaa
     3601 ctccggtttg ttggcgaaaa agttcggctt tatgcgcgat ctcttcagca tctggtacag
     3661 taccggtaac atccgtaaaa agtgtcttca ttattggaat taagggctta ctgattgccg
     3721 tcagaggcag gaacatcagt aaactgagca gtatcatgat ttttttcatg ggcgggactt
     3781 ccgtttcctg cgtgtctttt tagaggaggg catactgatt aataaaccga cggcgataaa
     3841 aataacgccc ataatgccga taaaccccca tttaaaaaaa ttaacccacg ggttatttat
     3901 ctcagccttt tcaggatgtc ctggagaata ataaacatta actttatcgc cttcgctgtc
     3961 ttcataaaaa tcgctgccaa tactcgaatt gaaaattagc gtgtaatctg gcgtcggacg
     4021 aaaggcaacg acgggatacc acgaaccatt tttacccgtg cgatgagaat gactggagtg
     4081 ccagacggta tcaacaatga cgccttcagt atgaatcgcg tctttgctat aggtgaactg
     4141 gttataaaag ataacaacag aaatgaggat cagtaccaga ccgataccga agaatatacg
     4201 aaagaaaact ttactgtcct gactcattag ccacatcctt gctaaataaa aaacgggcgg
     4261 taataccacc gcccgcttgc tgaattaacg tcctgctttc agcttctgat aatactcttc
     4321 ataaatgctg ctggctgcgc caacgtcatt ctgccattca ccatttttaa tggtttcagc
     4381 atccgggtag agcgttttgt cgttcgccac ttcaggactt aacagcttac gcgccgcaag
     4441 gtttggcgtt ggataaccga tagtttcagc aacctgtttt gccacatcgg ggcgcagcag
     4501 gaagttgatc aatttcagcg caccttcttt gtttttggca tttgccggga tcgccaggct
     4561 gtccatccag aaaatgccgc cttctttcgg ccacaccacg tcaattggcg tacccgcctg
     4621 ccgtgcaacg aaagcagaac cgttccagat catgccgagg ttaacttcgc cttccatgta
     4681 cgggttagcc gggttatcgg agttaaacgc tgcgacgttt ggcatcagtt ttttcagctc
     4741 gttatatgca gcttcaatct ctttcggatc ggtagtgtta ccggagtagc ccagtttgcg
     4801 cagcgccatc tggaacactt cacgggcatc gtcggtcagc agcaggctgc ctttgtactc
     4861 aggtttccac agatcggccc agctggtgac agatttcgga tccaccgcat caccgttaac
     4921 gccaatcgcc gtcgcacccc agatatacgg aatggagtag tcgttattcg ggtcaaacgg
     4981 cttgttgagc atgtctggat cgaggttgct gaaattgctt aacttcgact tgtcgatctt
     5041 ctggatcatc ccttctttac gcattttatc gacgtaatag gttgaaggaa ccaccaggtc
     5101 ataggcaccg tctttgtacg ttttcagctt cgcgtacatg gtttcgttcg actcgtaagt
     5161 cgaatagata accttaatac cggtttcttt ggtgaactgt tcaagcagtc ctggcggcac
     5221 gtactcggtc cagttgtaga aatacagcgt gttgttgtca tcggcgtgag cggcgctcat
     5281 gcccagtgcc agagcacccg ccgcgagcag gtggcgtgac cattttttca tttaacgtcc
     5341 cctgtgttac ctttcgtttt atcacgagca ataagctggc tggcaattac catcaccagc
     5401 gacagcacca gtaatatggt tgccagcgcg ttaacttccg gcgatacgcc gactttgacc
     5461 atcgaataaa tttttaacgg cagaatttca taactcggcc cggtgacaaa cgaagaaact
     5521 accacatcgt ccatcgacag ggtaaagctt aacacccagc ccgccgctac cgctggcatt
     5581 gccagtggca gaatgatttt tcgcagaatg gtaaattcgc tggcaccgag atctttcgcc
     5641 gcttccagca ttcgcacgtc aaaacctttc aggcgcgaat acactgtcac taccacaaat
     5701 ggcaggcaga aggtgatatg cgagaacagc agcgaccaga agccaagctg aatacccagc
     5761 agcataaaca gcaccagcag cgaaatcgcc atcacgatat ctggtgacat catcaccaca
     5821 aacagcattc cgctaacgaa cggcttacca cgaaagcgat aacggtacag cgcaactgcc
     5881 gtcagtgaac cgataagcgt agcaaacgtc gccgaaaaca ccgccattgt tagtgaatgc
     5941 tgcgctgcct gtaacaggct gtcgttgttc atcagcaggc tataccattt ggtggtaaaa
     6001 ccttgccagt tgatgccaaa gcgcgagctg ttaaaggagt tcacaatcaa aataatgatt
     6061 gggatataca ggtacgcgta gatagcggtc ataaaaccgc cgcgaagcag tcgaccgatc
     6121 attcgagttc caccttctta ttcagcaaac gagaagcgcg ccagtaaacc agcaacatca
     6181 ggcccattac gatagtcagc gtaatgctgg tggctgcacc aaacggccag tcacgaatat
     6241 tgaggaactg taccttgatg acgttaccga tcagcaggtt tttcgcaccg cccatcaggt
     6301 cggacacata gaacaggccc atcgctggca gcatcaccag cagacatccg gcaataatcc
     6361 ccggcatcgt cagtggaata atgatgcgga taaaagtctg taacttgctg gcaccgagat
     6421 cgcgcgccgc ctcaagcaac ggcttatcca gtttttcgat actggagtac agcggcatca
     6481 ccataaacgg cagcagaatg taaaccagac cgataatcac cgcactgggc gtgaacatga
     6541 tgcgaattgg tgtatcaata acgcccagcc agagcaaaaa ctcgttgaga tagcctttgg
     6601 tgctgaggaa aattttcagc ccgtagatac gaatcaatga gttggtccag aacggaacaa
     6661 tcagcagaaa cagcagcagc ggacgcacct tgtgtggcaa cttcgccaga aaccaggcaa
     6721 aaggatagcc cagcaccagg caggcgaggg tggcgatcag cgccatattc agcgagtgca
     6781 atagcacttc aaaatagagc ggatcgagca gacgcgtgta gttatccagc gtaaagacca
     6841 ttttgacgaa actggcgtcg tcgcgggtca aaaagctggt gccaatgatc atcaggttgg
     6901 gcagaaagac aaacaacaca agccaaccga caatagtgac aatcactaca ttctggaact
     6961 tacttgtgtt cttcatcagc cagtacgacc tcccagcttt ctacccaatt aatggccatt
     7021 ttttggtcga gagagtggtc aaagtcaggg tcgtcttcat tgaagaattc gctgaccatc
     7081 accatcttgc cattttccag ttcaacaacc gactccagcg tcatgccttt gtagttacgc
     7141 tcgcgaacgt aaccaatcag cccttcagcg tggttgtcgt cgttaatctc ttcaacacgt
     7201 aagtcttccg ggcgcagcag aacatgcagt ttttgccccg gttcaacggc gaagttaacg
     7261 tagatattac attcgcggcc ttcaacgttg gcgcgtaccc gctgctcgtc gagacgttcg
     7321 atgacagtgg cgttaaacat attgatttcg ccaataaacc cagcaacaaa caggtttttc
     7381 ggctcttcat agatttcacg cggcgtgccg tcttgctcaa tgcgaccatc gcgcatcacc
     7441 acaatcctgt ccgacatggt gagtgcttct tcctggtcgt gagtcacgaa gacgaatgta
     7501 atgccaagct tacgctgtaa cgctttcagc tcgttctgca tttgcttgcg cagtttgtaa
     7561 tccagcgctg agagcgactc atccagcaac aacagacgag gcttgttaac caccgcgcgg
     7621 gcaatggcga cgcgttgctg ttgaccacca gagagctgat gcggtttgcg ttgagcgaag
     7681 gtttccagct gcaccatccg cagggcttcc atcacgcggg gcgtaatttc agcagcgggg
     7741 gttttttgca tgcgcaaccc aaaggccaca ttttcgaaca cggtcatgtg ggggaaaagt
     7801 gcgtagcttt ggaaaacagt attcacatag cggttttccg ccggaacgtg ggtgatgtcc
     7861 tcgttatcca gcatgatgcg tccggaatca acagtttcca gacctgcaat caggcgaaga
     7921 acggttgttt taccgcagcc agaagggcca agcagcgtga ggaactcgcc attgttgata
     7981 gtcagatcca gctggggaat gacctcttta ccatcaaagc atttgcgaat tcccgccaat
     8041 tgcaccagcg gtgaaagcga actcggttgt ttattcaatt ttttactctg tcccatgtaa
     8101 acgcaacgga tggcttaccg atgcggggtt tgtggttaac caccttggtg actcttaatg
     8161 agggcggtaa ttctacggca aaccgcttga atcgccaatc tttgttgtga attactggct
     8221 tagctttata ttcattaagg taatgctgat aaatattccc gcttgcaggg gtaaaagtga
     8281 cctgacgcaa tatttgtctt ttcttgcttc ttaataatgt tgtcacaaaa agtgagggtg
     8341 actacatgga taaactactt gagcgatttt tgaactacgt gtctctggat acccaatcaa
     8401 aagcaggggt gagacaggtt cccagcacgg aaggccaatg gaagttattg catctgctga
     8461 aagagcagct cgaagagatg gggcttatca atgtgacctt aagtgagaag ggcactttga
     8521 tggcgacgtt accggctaac gtccctggcg atatcccggc gattggcttt atttctcatg
     8581 tggatacctc accggattgc agcggcaaaa atgtgaatcc gcaaattgtt gaaaactatc
     8641 gcggtggcga tattgcgctg ggtatcggcg atgaagtttt atcaccggtt atgttcccgg
     8701 tgcttcatca gctactgggt cagacgctga ttaccaccga tggtaaaacc ttgttaggtg
     8761 ccgatgacaa agcaggtatt gcagaaatca tgaccgcgct ggcggtattg caacagaaaa
     8821 acattccgca tggtgatatt cgcgtcgcct ttaccccgga tgaagaagtg ggcaaagggg
     8881 cgaagcattt tgatgttgac gccttcgatg cccgctgggc ttacaccgtt gatggtggtg
     8941 gcgtaggcga actggagttt gaaaacttca acgccgcgtc ggtcaatatc aaaattgtcg
     9001 gtaacaatgt tcacccgggc acggcgaaag gcgtgatggt aaatgcgctg tcgctggcgg
     9061 cacgtattca tgcggaagtt ccggcggatg aaagcccgga aatgacagaa ggctatgaag
     9121 gtttctatca cctggcgagc atgaaaggca ccgttgaacg ggccgatatg cactacatca
     9181 tccgtgattt cgaccgtaaa cagtttgaag cgcgtaaacg taaaatgatg gagatcgcca
     9241 aaaaagtggg caaagggtta catcctgatt gctacattga actggtgatt gaagacagtt
     9301 actacaatat gcgcgagaaa gtggttgagc atccgcatat tctcgatatc gcccagcagg
     9361 cgatgcgtga ctgcgatatt gaaccggaac tgaaaccgat ccgcggcggt accgacggcg
     9421 cgcagttgtc gtttatggga ttaccgtgcc cgaacctgtt cactggcggt tacaactatc
     9481 atggtaagca tgagtttgtg actctggaag gtatggaaaa agcggtgcag gtgatcgtcc
     9541 gtattgccga gttgacggcg caacggaagt aagcgaaaag ggatgcggca tgtgatgccg
     9601 catccggctt aaatccaaac ttacccttcg aagaaccagt atccgctatt gaccagcgcc
     9661 gcgagcatcg cgaggaatga cggatcttcc agcgcatcgc caaaattctc cgcagtcagc
     9721 gcaatgttgc tggcgagtgc atccagtgcc ggacggtgcg gggaatcgat cttctcacca
     9781 ttggcataca cgtcgtcgcc aatgcgcaat acgcgtagac cacccaggcg caccagcaca
     9841 tcaccttgtt tcagcgcatc gtagatttca tccggctgat aaggcggctc tggcggcgcg
     9901 atatccagtt catgacgtga ctgggatata aactccccaa accattgctt aaagtgttcc
     9961 ggctggttga tcaattcgag catcatctca cgcagtttat ccatctcttg cggcagaaca
    10021 tccgcaggat gagcgcgagg tggaacatca ggatcgctgt agtagttgcc gcccagttca
    10081 cgttgcagca cataatcggc aaatccgcta atcagttccc gcgtatttgg cgcgcgaaaa
    10141 cctacggaat agttcatcgc attttccagc gcgtagcctt catgcgggaa tcctggcgga
    10201 atataaagaa tatcgccagg ctccagctct tcatcgatga tggcttcgaa cggatcgacc
    10261 tgtaacagat ccgggtgcgg gcagtgctgt ttcatttgca gcttttcgcc cactcgccag
    10321 cgacgacgtc cggtaccctg aatgataaac acgtcgtact gatcgagatg cgggccgacg
    10381 ccgccgcctg gtacagaaaa agagatcatc agatcatcaa tacgccagtc cggtagttca
    10441 cggaacggtc gcattagcgc ggcggtcggc tcatgccagt ggttcactgc ttgcactaac
    10501 aatgaccagt tagtttcacc gagatgatcg tagctttcga atggaccgtg gctgacctgc
    10561 catttgccat cctgatgact gaccagtcga ctgtcgactt cgctttccat cgccagaccc
    10621 gccaactcgt ctggagagat cgggtcaata aaattattaa agccgcgttt taacaccacc
    10681 gggcgtttct gccagtgacg ttcaagaaaa tcgggccagt taagagtgag ttggtattcc
    10741 atgttaagca tccacaggct ggtatctgca accgattata acggatgctt aacgtaatgc
    10801 gtgaagtatg gacatattta ttcatctttc ggcgcagaat gctggcgacc aaaaatcacc
    10861 tccatccgcg caccgcccag catgctctct ccggcgacga ttttaccctc atattgctcg
    10921 gtgatttcgc gggctaccgc cagccctaca ccttgcccag ggcgtaaagt atcaacccgt
    10981 tgaccacggt cgaaaatgac ctctcgcttg cttaatggaa taccggggcc atcatcctcg
    11041 accacaatat agagatgctc gtcggtttgc cttgcagaaa tttcgacaaa ctcgaggcaa
    11101 tatttacagg cattatccag cacattgccc atcacctcga caaaatcgtt ctgctcacca
    11161 acaaagctga tctctggcga aatatcgaga gagatattga cccctttgcg ttgatacact
    11221 ttgttcagcg ccgaggtgag cttgtccaac agtggggcga ccggatgcag ctcgcggcta
    11281 agcaatgtcc cgccgcgcat actggcacga tgcaggtagt agccaatttg ctgtgaaatg
    11341 cggctgattt gctccagcat taccggctca gcatcactga cgctcatctt ctcactacgc
    11401 agagaacgca gcgtactttg cagcaccgcc agtggcgttt tcagactatg ggtcaggtcg
    11461 gtgagcgttg tacgatattt gtcgtaacgt tcgcgttcac tttttaacaa tcggttcagg
    11521 tttcgtacca gactggtcag ttctcgcgtt gtggctggat tgagcaattc gcggttatgt
    11581 tcttccagtt cgcggacttc ttttgccagg gcttcgatgg ggcgtaaact ccaccaggcg
    11641 gcgacccaca gcagcgggat cactaacagc agattggctg agagcacata gataaaccag
    11701 ctccagacca tataggaact ttttagctcc accggaatgg tatccaccac cacaatggtt
    11761 aactttggca tccgcgatgt tgccgggtag acgtttaccg ctaccgagtg ggtcatctcc
    11821 gcgtcgtcat catcttcccg cacttcctgc aactgttgct gtatcgaatg atctccactc
    11881 agcaagaggc tggtatcgtt aacatccgct tcaatttcat gaaaaccatt cgatttcagc
    11941 cagtcaggct ggatcatctt catcagccag ggcacgtcac gttgcgccca taaaagctgc
    12001 ccgttctcat cataaattag cgtcatggtg gggctttgct tgtcgatatt ttcgggtaac
    12061 tcgacatgca acttattgtt ttcccacttc gcaagggtat agaacagatt gctctcgcca
    12121 cgtaacagcc gaaacgtggt tttatcgaaa ctgacgctat aaccgatcag cgcaaccatt
    12181 ccgtaggcaa gcgaaagcac caatactact gctgccgttg ctaacagaaa acgtacccgc
    12241 agcgagagcg ggaaaaaaag acgcagtaat tttttcatca gcgcaattcg aacagatagc
    12301 cctggccgcg aacggtggta atcacttctt gcggatattg tgcctgaatt tttttgcgca
    12361 gacgtcccat cagtacatca atggtatggc tttcccgcag ctccgcatcc gggtagagtt
    12421 gtagcattaa cgaatctttg ctgaccactt tgccattatt gcgtatcaac gtttccataa
    12481 tggtgtattc gaacgcggtt agtttgatca cttcgtcatt aatagataat tcacggcggg
    12541 agagatcaac ctgaaacggt ggtagcgaaa tgacctgtga agccagaccg ctattacgcc
    12601 gcattaatgc ctgcattcgc gccatcacct cttcaatatg aaacggttta gtcacgtaat
    12661 catcagcacc ggcacttaat acttcgactt tgtcctgcca gctttcacgg gcggttaata
    12721 ccagaatcgg cagtgaaaca tcattgctac gccagcggcg aatcagtgac agaccatcct
    12781 cgtcaggcaa tccgagatcg acaatcgcaa tatccggtaa atgttcattg agataataat
    12841 cggcttcttt ggcatcttct gcgtcatcga cctgatgacc agcatcctga atctgaactt
    12901 taagatggtg acgtaacaac gcattgtctt caacaaccag tacgcgcatt tttatttctc
    12961 cctgtcttaa ttattaaaat agtgtaacgc gattatgtgg ttatgggggt aaacattaaa
    13021 taaaccagcg ggggagggga ggtaaagtga aaaaataaaa agcggataat cttaataagc
    13081 aggccggaca gcatcgctat ccggcactga tacgagttta tttcagctca tcaaccatgg
    13141 tgatggcgcg accaatgtag tttgccggcg tcatcgcttt aaggcgggct ttctcttctt
    13201 ccggcagcgc cagaccgtcg ataaactgct tcatgccttc ggcgtcaacg cgcttaccgc
    13261 gagtcagctc tttcagcttc tcgtacggtt tttcgatgcc atagcgacgc ataactgtct
    13321 ggattggctc agccagcact tcccagttgt gatccagttc atccagcaga tggccacggt
    13381 tcacttccag tttgctcacg cctttcaggg tggattgata cgcaatcagc gcataaccga
    13441 tacccacgcc gaggttacgc agcacggtgg agtcggtcag gtcacgctgc cagcgggaaa
    13501 ctggcagttt gcttgccagg tgctgcaata ccgcgttgga aaggcccagg tttccttcgg
    13561 agttttcgaa gtcgatcggg ttaactttat gcggcatggt ggaagaacca atctcaccag
    13621 caatggtttt ctgtttgaag tggttaaggg cgatataacc ccagacgtca cggtcaaagt
    13681 cgatcagaat ggtgttgaag cgcgcaacgc aatcaaacag ttcggcaatg tagtcgtgcg
    13741 gttcgatctg ggtggtgtac gggttccact gaatacccag cgaggtgacg aactcttcgc
    13801 tgaactgatg ccagtcaact tccgggtaag cggcgatgtg ggcgttatag ttaccgaccg
    13861 caccgttgat tttgccgagg atctccacct ggttaagctg gcggtactgg cgctccatac
    13921 ggtaggcgac gttagccatc tctttaccga tggttgacgg cgtggctggc tgaccgtggg
    13981 tacgggacag cagcgggata tcgcggtact gagcggcgag atctttaatg ccatcaatca
    14041 gttgacgcca gtacggcagg atcacttcat cacgcgcggt tttcagcatt aatgcgtggg
    14101 agaggttatt gatatcttcc gaagtacagg caaagtggat gaattcagaa accgcgtgca
    14161 gttccgggat ctccgccact ttttctttca ggaaatactc aaccgcttta acgtcgtggt
    14221 tagtggtacg ctcgatggtt ttgatgcgtg cggcatcttc ttcgctgaaa ctggcgacaa
    14281 ttgcatcaag gtaaccgatt gcgtcggcag caaaagcagg aacttccttg atcgctgcgt
    14341 gcgcggccag tttttgcagc caacgtactt caacttgtac acggaatttc agcaaaccat
    14401 attcgctgaa aatcccgcgc agcgcgctga ctttatcgcc gtagcgtcca tcgacagggg
    14461 aaacggcggt cagtgaggat aattccatag atcacaactc cggggttaaa tgagcaagaa
    14521 tttgttttgc ctgagtggtc aggcgattac gagaaaacat cagttgcaga cgtccgccgc
    14581 cgacctggtg ccagagcacg gcggcgcgaa tgcctgccag cagggtagcg cggactttcg
    14641 cctgtacctg tggactttgc agtacagcag gggaaccggt gacctgaatg cgtgggccaa
    14701 gcgggctaat cacatcaaca tagatagcag ccatcgcgct catcagcgtt tcggactgta
    14761 aatcgaagtg ttcgagctgg cgttgcaggc cgttgatccg gttgcccaga gtgtcgagcg
    14821 cacctttcgc tgaggagagt ttgcgctcaa gcaccatcag actgagtgtg tagcgggtta
    14881 attcggcgtt taagccctgg cgactgctgg cattgagtac gccgagcaag gtttccagcc
    14941 cgacgcgcag gttggcttcg ctgccgccaa aaaccgccag cgttgagctg gggttcatat
    15001 cgataatact gttgagtgag acgtgtagcg catcgccatc acaatgcccc tggtgagcga
    15061 gttgttgcac caggcgtgcc gactgacaaa taccggccag ggcgagggtg atgtcatagt
    15121 aattctttgc cacgttcact gcttccttgt tttaagtaaa gataataatc agaccggcag
    15181 cggcagacgc tgctcaataa taccgccacc gaggcacacc tcaccgttat aaaaaacggc
    15241 agactggccc ggcgtcacgg cggcaaccgg ctcatcgaaa atcacttcaa tgcgatcatc
    15301 gtccagcgcc ttgacggtgc aggggatatc ggtctggcga tagcgggttt ttaccgtgca
    15361 acgcatagtg ccggtgaatg gttcgcgatc gacccagtgc agctgctggg caatcagacc
    15421 gacagacatc agacgcgggt gttcatggcc ctgcgcaaca atcagaatgt tgttttcgac
    15481 gtctttatcg accacatacc acggttcttc ggtgccttct ttggtgccac cgatacccag
    15541 acctttacgc tgaccgagag tgtgatacat cagcccctgg tgctcaccaa tttcatcgcc
    15601 atcgacggta atgattttgc ccggttgcgc cgggagataa cggcccagga actcgcggaa
    15661 tttacgttcg ccaatgaagc aaatgccggt agagtctttt ttcttcgcgg tgaccagacc
    15721 aagatcttca gcaatcttac gcacctgcgg tttttccagt tcgccgaccg ggaacaggct
    15781 ttgcgcaatc tgctcatggc taagcgtata aaggaagtag ctctggtctt tattgctgtc
    15841 cagaccacgc agcaggcggc tcttgccatc gacatctgcc cgacgtacgt aatgaccggt
    15901 agcgatataa tcggcaccta aatcttcggc ggcaaattcg aggaaggctt taaatttgat
    15961 ctctttgttg cacagaatat ccggattcgg cgtgcgaccg gctttatatt cggcaaggaa
    16021 cagttcgaag acgttgtccc agtactcggc agcaaagtta acggtgtgca gttcaatgcc
    16081 gagcttgtcg cagacagcct gggcatcagc cagatccgct gccgctgtgc aatattcctc
    16141 accgtcgtct tcttcccagt tcttcataaa caggccttcg acctgatatc cctgttgttg
    16201 caacagccag gcagaaacgg aggaatcgac accgccggac atgccgacga ttactttttt
    16261 tgcggtttca gacattggat cactcacgac attgaacttc aaggcggcgt attctatcac
    16321 gcagcctttt agatgacacc ctttgtaaaa ggccagttaa aatcgccaat catctccagc
    16381 ggatagcgtt gcccgctttg ataacaacga atactttccg ccaccagcgg cgaacgaaga
    16441 tttgacgcct gtaaaatttc ttcggcgctg acccaacggc agcagtcgat atcgctgtca
    16501 tgaggctggg tcgggcatat ttgctcaagc tcaatggcaa agaggaaacg caaaaacggc
    16561 gttttatctg gcgcaatcca ctgatgcata cgaataaagt gttgcggctg cgcgctgatg
    16621 ccggtttctt cccacagctc acgggcggcg gcttccacta aggtttcatc ggcttccaga
    16681 tgcccggcag gttggttcca taacgcttta ccattaatcg tctcttcaac gactaaaaat
    16741 ttgccttctg cgtgcaccac gcaagcaacg gtaacgtgcg gtttaaacat tttgcgtcct
    16801 taatctgtcg cttctcgcca ttcgccgttg gcaagattat ccaaagagta atcacccatc
    16861 gcatagcgaa tcagtcgcag cgtggggaaa ccaacatggg cggtcatgcg gcgcacctgg
    16921 cgattacgtc cttcatataa agtaatcttc agccagctgg tgggaatact tttacgttca
    16981 cgaatcggcg gattacgcgg ccataaccac tccggctctt caaccaggtc cacgcctgcg
    17041 ggcagggtag ggccatcatt taaggtcacg ccattgcgca aggcctcaag tgcgtcttgt
    17101 gtgggaatac cttccacctg cacataatag attttcccgg tgcgtttacc cggctgggtt
    17161 aaacgcgcct gcaacgcacc gttattggtc agcaccagta acccttcgct atcgcggtca
    17221 agacgacctg ctgcataaac gccctgaacc gggatgaatt cttttaatgt tttgcgtccg
    17281 gcttcatcgg tgaactgcgg aagaacatcg tagggtttat tgaacaggat cacacgcgtg
    17341 ggctggtttt caggtttacg cctggtagaa cgttgcgagc tgaatcgctt aacctggtga
    17401 tttctaaaag aagttttttg catggtattt tcagagatta tgaattgccg cattatagcc
    17461 taataacgcg catctttcat gacggcaaac aatagggtag tattgacaag ccaattacaa
    17521 atcattaaca aaaaattgct ctaaagcatc cgtatcgcag gacgcaaacg catatgcaac
    17581 gtggtggcag acgagcaaac cagtagcgct cgaaggagag gtgaatggaa agtaaagtag
    17641 ttgttccggc acaaggcaag aagatcaccc tgcaaaacgg caaactcaac gttcctgaaa
    17701 atccgattat cccttacatt gaaggtgatg gaatcggtgt agatgtaacc ccagccatgc
    17761 tgaaagtggt cgacgctgca gtcgagaaag cctataaagg cgagcgtaaa atctcctgga
    17821 tggaaattta caccggtgaa aaatccacac aggtttatgg tcaggatgtc tggctacctg
    17881 ctgaaaccct tgatctgatt cgtgaatatc gcgttgccat caaaggcccg ctgaccactc
    17941 cggttggtgg cggtattcgc tctctcaacg ttgctctgcg ccaggaactg gatctctaca
    18001 tctgcctgcg tccggtacgt tactatcagg gcactccaag cccggttaaa caccctgaac
    18061 tgaccgatat ggttatcttc cgtgaaaact cggaagacat ttatgcgggt atcgaatgga
    18121 aagcagactc tgccgacgct gagaaagtga ttaaattcct gcgtgaagag atgggcgtga
    18181 agaaaattcg cttcccggaa cattgcggta tcggtattaa gccgtgttct gaagaaggca
    18241 ccaaacgtct ggttcgtgca gcgatcgaat acgcaattgc taacgatcgt gactctgtga
    18301 ccctggtgca caaaggcaac atcatgaagt tcaccgaagg cgcgtttaaa gactggggct
    18361 accagctggc gcgtgaagag tttggcggtg aactgatcga cggcggcccg tggctgaaag
    18421 ttaaaaaccc gaacaccggc aaagagatcg tcattaaaga cgtgattgct gatgcattcc
    18481 tgcaacagat cctgctgcgt ccggctgaat atgatgttat cgcctgtatg aacctgaacg
    18541 gtgactacat ttctgacgcc ctggcagcgc aggttggcgg tatcggtatc gcccctggcg
    18601 caaacatcgg tgacgaatgc gccctgtttg aagccaccca cggtactgcg ccgaaatatg
    18661 ccggtcagga caaagtaaac ccaggctcta ttattctctc cgctgagatg atgttacgcc
    18721 atatgggttg gactgaagcc gcagacctga ttgttaaagg tatggaaggc gcgatcaacg
    18781 cgaagactgt aacctatgac ttcgaacgtc tgatggaagg cgctaaactg ctgaaatgtt
    18841 cagagtttgg tgatgcgatc atcaagaaca tgtaatctct acatgtgtta aatattgaaa
    18901 cgggcgtata acacgcccgt tgttttattt atgtggatat tattaatagc atgtcgagaa
    18961 tatttatatg aagcccatta cttgagtcca tatgggcata tttttataat gcaactatta
    19021 tgcaaacatt tatttgttat tttgctttct cctggaggac actcttgact gcttttgagt
    19081 aaactccata aatccttgtt gaatgatgcg atgttataaa tagtaatagg atattcttta
    19141 tccttaagga taataccaga cttaaccggt gtaaatatcc tgccaggagg gagaaatata
    19201 gtagattgat accagatgat cattttcata tcaccccata tggctgaaaa agatatgccg
    19261 catgtaggtt gaattaccgt gtcaattact atccacttca tttgttatgt cttatcccac
    19321 ggtgcttaat atagttcatt tggatgttca tttcttgatt ttgcatatgt gtatattact
    19381 cccaaaaaaa taaattaatt aaaaattaat gtcattatct atataaaaca aaatttaaag
    19441 caaggaatct caatagagat taaacaaaat gagatttagt gaaaacatta aattattcac
    19501 ttcgttttag atttgtttag ctaaaatgtt atacattcaa atgactgaat attctgtaat
    19561 taaaacatag cctttatgct actttgagcc aatttgctaa acattatggt tggcctttta
    19621 atataacgat aatgatgaat ataagcatga catgagaata atgtttcaat tttttagtta
    19681 tataggaatg gtttgaccca ttcctggcta aaatacatat aaccggatga tgactaaacc
    19741 aaaacacatg tgcgttaagt attgaaacgg gcgtatggca cgcccgttgg ttttataaat
    19801 atgttaaccg ttataaaata acgtatagaa agtcaagtga tcacatttca aatatcaagt
    19861 tgatagtatt gatctggtga ttatttatgg gtggcaataa aaagatagta ttactcatcc
    19921 atatgaatag tctctgcact tttatttcca ttatgctaat gccttactga attatgaagc
    19981 attttttaag catccaactt tagctagatt aatggtttat tgttttctac atcttcaata
    20041 tataaaagcg tattatcaat ggcgtagtaa ctgcgtttgt tatgattaac atcagtaacc
    20101 caccggaaaa cgcccgcgtc tgccagtgtt gaacagtatt cccgaaatgt agattttccg
    20161 caaatatgaa gcaatgcggc ctcttttatt ttagcagggt tcttggtcgt actaactttt
    20221 aacaggttcc tggttcctct taataacaaa accgtgtcat cgtgagtaat aattctgatg
    20281 ttatccgtag ctagataata aatgtaatgt gcaatacggt gatgttttaa ttctgaataa
    20341 aaccaggaga agttttgctc ttttctcact tgctcaaaca tcttttgaaa aacaacgacc
    20401 tgatccatca ggataataac ctcttgttag ttgtgagatt gcgtagtgtg cacgatcggt
    20461 tttaccactt caatctggtc tgtcctttgg ctgtgatatg tacaaagtgt gaaaggggga
    20521 ttacctgaat tctcccggtg aacattttgt aatggaccag ctccggtaca aacgctgttg
    20581 tgggttcaga ttataacatt ctgtctaagg ggcgggataa aggtgaaatt agggggcatg
    20641 gaagatgact ttataacctt gctcacccca gtgttgtaaa agttcgtttt gccttctcgt
    20701 tggtgccatg cctgtccaga caatcaatgt ttgcgtcggg aacagttccg ggcgcggcga
    20761 ttcaatggga tcagcaagaa cagaaatgtg ccatcctgac aatgataaac gccatgcttc
    20821 cagccacaat cgtgctctct cttcaacatt ccacgccatc agaatggctt ctttaccggg
    20881 cttacggcgc atttcgaaaa gcgaggctgc tgcgtattca attaatgcgc cgtcaaacat
    20941 actgctcata atgcgggagg tgttgtgatc aagcacgaga cgctggcgaa caggaaggta
    21001 aacatgatta atcaattgat ctactgggta ctctcggccc agtgaaataa ttctcgcgcg
    21061 tagtttggca ggattagcca tacgaagaat tgacatcatc tcttcttgca ggcggctcca
    21121 gtcatcttcc gtatcctggc tggtggtttc cagtaatgct ttaactttgc ctacagggac
    21181 gccattactt atccaacgct tgatctcttc gatgcgttgt atgtcttctt ca
//
LOCUS       DAMZFT010000083        20901 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.083, whole genome shotgun sequence.
ACCESSION   DAMZFT010000083 DAMZFT010000000
VERSION     DAMZFT010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20901)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20901)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20901
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.083"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(<1..460)
                     /locus_tag="PN977_003205"
     CDS             complement(<1..460)
                     /locus_tag="PN977_003205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6043215.1"
                     /translation="MAISTPMLVTFCVYIFGMILIGFIAWRSTKNFDDYILGGRSLGP
                     FVTALSAGASDMSGWLLMGLPGAVFLSGISESWIAIGLTLGAWINWKLVAGRLRVHTE
                     YNNNALTLPDYFTGRFEDKSRILRIISALVILLFFTIYCASGIVAGARLFE"
     gene            complement(619..828)
                     /locus_tag="PN977_003206"
     CDS             complement(619..828)
                     /locus_tag="PN977_003206"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6043216.1"
                     /translation="MMLQLCATMLNVTCVASLKMNEMLIKEIDMTGIKKITQTFSLRQ
                     LTFLKGATAKNVRECNLMKNSVAEH"
     gene            883..4845
                     /gene="putA"
                     /locus_tag="PN977_003207"
     CDS             883..4845
                     /gene="putA"
                     /locus_tag="PN977_003207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trifunctional transcriptional regulator/proline
                     dehydrogenase/L-glutamate gamma-semialdehyde
                     dehydrogenase"
                     /protein_id="HDI6043217.1"
                     /translation="MGTTTMGVKLDDATRERIKSAATRIDRTPHWLIKQAIFSYLEQL
                     ENSDTLPELPALLSGAANESDEAPTPAEEPHQPFLDFAEQILPQSVSRAAITAAYRRP
                     ETEAVSMLLEQARLPQPVAEQAHKLAYQLADKLRNQKNASGRAGMVQGLLQEFSLSSQ
                     EGVALMCLAEALLRIPDKATRDALIRDKISNGNWQSHIGRSPSLFVNAATWGLLFTGK
                     LVSTHNEASLSRSLNRIIGKSGEPLIRKGVDMAMRLMGEQFVTGETIAEALANARKLE
                     EKGFRYSYDMLGEAALTAADAQAYMVSYQQAIHAIGKASNGRGIYEGPGISIKLSALH
                     PRYSRAQYDRVMEELYPRLKSLTLLARQYDIGINIDAEEADRLEISLDLLEKLCFEPE
                     LAGWNGIGFVIQAYQKRCPLVIDYLIDLATRSRRRLMIRLVKGAYWDSEIKRAQMDGL
                     EGYPVYTRKVYTDVSYLACAKKLLAVPNLIYPQFATHNAHTLAAIYQLAGQNYYPGQY
                     EFQCLHGMGEPLYEQVTGKVADGKLNRPCRIYAPVGTHETLLAYLVRRLLENGANTSF
                     VNRIADTSLPLDELVADPVTAVEKLAQQEGQTGLPHPKIPLPRDLYGHGRDNSAGLDL
                     ANEHRLASLSSALLNSALQKWQSLPMLEQPVAAGEMSPVINPAEPKDIVGYVREATPR
                     EVEQALESAVNNAPIWFATPPAERAAILHRAAVLMESQMQQLIGILVREAGKTFSNAI
                     AEVREAVDFLHYYAGQVRDDFANETHRPLGPVVCISPWNFPLAIFTGQIAAALAAGNS
                     VLAKPAEQTPLIAAQGIAILLEAGVPPGVVQLLPGRGETVGAQLTGDDRVRGVMFTGS
                     TEVATLLQRNIASRLDAQGRPIPLIAETGGMNAMIVDSSALTEQVVIDVLASAFDSAG
                     QRCSALRVLCLQDEIADHTLKMLRGAMAECRMGNPGRLTTDIGPVIDSEAKANIERHI
                     QTMRSKGRQVFQAVRENSEDTREWQSGTFVAPTLIELDDFAELQKEVFGPVLHVVRYN
                     RNQLPALIEQINASGYGLTLGVHTRIDETIAQVTGSAHVGNLYVNRNMVGAVVGVQPF
                     GGEGLSGTGPKAGGPLYLYRLLANRPESALAVTLARQDAEYPVDAQLKAALTQPLNAL
                     REWAANRPELQALCTQYGELAQAGTQRLLPGPTGERNTWTLLPRERVLCIADDEQDAL
                     TQLAAVLAVGSQVLWPDDTLHHQLVKALPSAVSERIQLAKAENITAQPFDAVIFHGDS
                     DQLRALCEAVAARDGAIVSVQGFARGESNILLERLYIERSLSVNTAAAGGNASLMTIG
                     "
     gene            complement(4885..5523)
                     /gene="rutR"
                     /locus_tag="PN977_003208"
     CDS             complement(4885..5523)
                     /gene="rutR"
                     /locus_tag="PN977_003208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator RutR"
                     /protein_id="HDI6043218.1"
                     /translation="MTQGAVKITGKRSRAVSAKKKAILSAALDTFSQFGFHGTRLEQI
                     AELAGVSKTNLLYYFPSKEALYIAVLRQILDIWLAPLKAFREDFAPLAAIKEYIRLKL
                     EVSRDYPQASRLFCMEMLAGAPLLMDELTGDLKALIDEKSALIAGWVKSGKLAPIDPQ
                     HLIFMIWASTQHYADFAPQVEAVTGATLRDEVFFNQTVENVQRIIIEGIRPR"
     gene            5811..6902
                     /gene="rutA"
                     /locus_tag="PN977_003209"
     CDS             5811..6902
                     /gene="rutA"
                     /locus_tag="PN977_003209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309285.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein A"
                     /protein_id="HDI6043219.1"
                     /translation="MKIGVFVPIGNNGWLISTHAPQYMPTFELNKAIVQKAEHYHFDF
                     ALSMIKLRGFGGKTEFWDHNLESFTLMAGLAAVTSRIQIYATAATLTLPPAIVARMAA
                     TIDSISGGRFGVNLVTGWQKPEYEQMGIWPGDDYFSRRYDYLTEYVQVLRDLWGSGKS
                     DFKGDFFTMDDCRVSPQPSVPMKVICAGQSDAGMAFSARYADFNFCFGKGVNTPTAFA
                     PTAARMKQAAEQTGRDVGSYVLFMVIADETDDAARAKWEHYKAGADEEALSWLTEQSQ
                     KDTRSGTDTNVRQMADPTSAVNINMGTLVGSYASVARMLDEVATVPGAEGVLLTFDDF
                     LSGIENFGERIQPLMQCRAHLPALTQEVA"
     gene            6902..7594
                     /gene="rutB"
                     /locus_tag="PN977_003210"
     CDS             6902..7594
                     /gene="rutB"
                     /locus_tag="PN977_003210"
                     /EC_number="3.5.1.110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxyureidoacrylate/ureidoacrylate
                     amidohydrolase RutB"
                     /protein_id="HDI6043220.1"
                     /translation="MTTLTARPEAITFDPQQTALIVVDMQNAYATPGGYLDLAGFDVS
                     TTRPVIANIQTAVTAARAAGMLIIWFQNGWDAQYVEAGGPGSPNFHKSNALKTMRKQP
                     QLQGKLLAKGSWDYQLVDELVPQPGDIVLPKPRYSGFFNTPLDSILRSRGIRHLVFTG
                     IATNVCVESTLRDGFFLEYFGVVLEDATHQAGPEFAQKAALFNIETFFGWVSDVETFC
                     DALSPTSFARIA"
     gene            7606..7992
                     /gene="rutC"
                     /locus_tag="PN977_003211"
     CDS             7606..7992
                     /gene="rutC"
                     /locus_tag="PN977_003211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein C"
                     /protein_id="HDI6043221.1"
                     /translation="MPKSVIIPAGSSAPLAPFVPGTLADGVVYVSGTLAFDQHNNVLF
                     ADDPKAQTRHVLETIRKVIETAGGTMADVTFNSIFITDWKNYAAINEIYAEFFPGDKP
                     ARFCIQCGLVKPDALVEIATIAHIAK"
     gene            8000..8812
                     /gene="rutD"
                     /locus_tag="PN977_003212"
     CDS             8000..8812
                     /gene="rutD"
                     /locus_tag="PN977_003212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001602165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein D"
                     /protein_id="HDI6043222.1"
                     /translation="MKLSLSPPPYADAPVVVLISGLGGSGSYWLPQLAVLEQEYQVVC
                     YDQRGTGNNPDTLAEDYSIAQMAAELHQALVAAGIERYAVVGHALGALVGMQLALDYP
                     ASVTVLVSVNGWLRINAHTRRCFQVREQLLHSGGAQAWVEAQPLFLYPADWMAARAPR
                     LEAEDALALAHFQGKNNLLRRLNALKRADFSRHADRIRCPVQIICASDDLLVPTACSS
                     ELHAALPDSQKMVMRYGGHACNVTDPETFNALLLNGLASLLHHREAACKELL"
     gene            8809..9399
                     /locus_tag="PN977_003213"
     CDS             8809..9399
                     /locus_tag="PN977_003213"
                     /EC_number="1.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonic semialdehyde reductase"
                     /protein_id="HDI6043223.1"
                     /translation="MNEAVSPGALSTLFTDARTHNGWRETPVSDETLRELYALMKWGP
                     TSANCSPARFVFIRTAEGKERLRPALSSGNLQKTLTAPVTAIVAWDSEFYERLPQLFP
                     HGDARSWFTSSPQLAEETAFRNSSMQAAYLIVACRALGLDTGPMSGFDRQHVDDAFFA
                     GSTLKSNLLINIGYGDSSKLYARLPRLSFEEACGLL"
     gene            9410..9904
                     /gene="rutF"
                     /locus_tag="PN977_003214"
     CDS             9410..9904
                     /gene="rutF"
                     /locus_tag="PN977_003214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309280.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization flavin reductase protein
                     F"
                     /protein_id="HDI6043224.1"
                     /translation="MNIVDQQTFRDAMSCMGAAVNIITTDGPAGRAGFTASAVCSVTD
                     TPPTLLVCLNRGASVWPVFNENRTLCVNTLSAGQEPLSNLFGGKTPMEHRFAAARWQT
                     GVTGCPQLEEALVSFDCRISQVVSVGTHDILFCAIEAIHRHATPYGLVWFDRSYHALM
                     RPAC"
     gene            9925..11253
                     /gene="rutG"
                     /locus_tag="PN977_003215"
     CDS             9925..11253
                     /gene="rutG"
                     /locus_tag="PN977_003215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415526.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization transport protein G"
                     /protein_id="HDI6043225.1"
                     /translation="MAMFGFPHWQLKSTSTESGVVAPDERLPFAQTAIMGVQHAVAMF
                     GATVLMPILMGLDPNLSILMSGIGTLLFFFITGGRVPSYLGSSAAFVGVVIAATGFNG
                     QGINPNISIALGGIIACGLVYTVIGLVVMKIGTRWIERLMPPVVTGAVVMAIGLNLAP
                     IAVKSVSASAFDSWMAVMTVLCIGLVAVFTRGMIQRLLILVGLIVACLLYGVMTNVLG
                     LGKAVDFTLVSHAAWFGLPHFSTPGFNSQAMMLIAPVAVILVAENLGHLKAVAGMTGR
                     NMDPYMGRAFVGDGLATMLSGSVGGSGVTTYAENIGVMAVTKVYSTLVFVAAAVIAML
                     LGFSPKFGALIHTIPAAVIGGASIVVFGLIAVAGARIWVQNRIDLSQNGNLIMVAVTL
                     VLGAGDFALTLGGFTLGGIGTATFGAILLNALLSRRLVDVPPPEVIHQEP"
     gene            complement(11336..11509)
                     /locus_tag="PN977_003216"
     CDS             complement(11336..11509)
                     /locus_tag="PN977_003216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="HDI6043226.1"
                     /translation="MANHRGGSGNFAEDRERASEAGKKGGQHSGGNFKNDPQRASEAG
                     KKGGKSSHGKSDN"
     gene            11882..12478
                     /gene="wrbA"
                     /locus_tag="PN977_003217"
     CDS             11882..12478
                     /gene="wrbA"
                     /locus_tag="PN977_003217"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HDI6043227.1"
                     /translation="MAKVLVLYYSMYGHIETMARAVAEGASKVDGAEVVVKRVPETMP
                     PQLFEKAGGKTQTAPVATPQELADYDAIIFGTPTRFGNMSGQMRTFLDQTGGLWASGA
                     LYGKLASVFSSTGTGGGQEQTITSTWTTLAHHGMVIVPIGYAAQELFDVSQVRGGTPY
                     GATTIAGGDGSRQPSQEELSIARYQGEYVAGLAVKLNG"
     gene            12499..12726
                     /locus_tag="PN977_003218"
     CDS             12499..12726
                     /locus_tag="PN977_003218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002898701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccJ family protein"
                     /protein_id="HDI6043228.1"
                     /translation="MPTQEAKAHHVGEWASLRNTSPEIAEAIFEVAGYDEKMAEKIWE
                     EGSDEVLVKAFAKTDKDSLFWGEQTIERKNV"
     gene            complement(12764..14005)
                     /gene="agp"
                     /locus_tag="PN977_003219"
     CDS             complement(12764..14005)
                     /gene="agp"
                     /locus_tag="PN977_003219"
                     /EC_number="3.1.3.10"
                     /EC_number="3.1.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glucose-1-phosphatase/inositol
                     phosphatase"
                     /protein_id="HDI6043229.1"
                     /translation="MNKTLIAATVAGIVLLASNAQAQTVPEGYQLQQVLMMSRHNLRA
                     PLANNGSVLEQSTPNKWPEWDVPGGQLTTKGGVLEVYMGHYMREWLAEQGMVKSGECP
                     PPDTVYAYANSLQRTVATAQFFITGAFPGCDIPVHHQEKMGTMDPTFNPVITDDSAAF
                     SEQAVAAMEKELSKLQLTDSYQLLEKIVNYKDSPACKEKQQCSLVDGKNTFSAKYQQE
                     PGVSGPLKVGNSLVDAFTLQYYEGFPMDQVAWGEIKSDQQWRGLSKLKNGYQDSLFTS
                     PEVARNVAKPLVSYIDKALVTDRTSAPKITVLVGHDSNIASLLTALDFKPYQLHDQNE
                     RTPIGGKIVFQRWHDSKTNRDLMKIEYVYQSAEQLRNADALTLQAPAQRVTLELSGCP
                     IDANGFCPMDKFDSVLNEAVK"
     gene            complement(14298..15554)
                     /locus_tag="PN977_003220"
     CDS             complement(14298..15554)
                     /locus_tag="PN977_003220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccE family protein"
                     /protein_id="HDI6043230.1"
                     /translation="MGSNIHGISCTANNYLKQAWHNIKKEHEKNQTYSITLFENTLVC
                     FMRLYKEIRRQKAEDYIPCLECESLEKEFEEMQNDNDLSLFMRTLRTNDTQIYSGVSG
                     GITYTIQYVRDIDIVRVSLPGRASESITDFKGYYWYGFMEYIENINACDDVFSEYCLD
                     DENISVQPKQINTPGISDLDTGIDLSGISFIQSEINKTYELKYAPVDGDGYCLLRAIL
                     VLKQHDYSWALGSHKTQKEVYEEFIKIVDKPTIEALVDAAFCDLREDVKTLFGVDLQS
                     DNKIQGVGSHMSWSFLFFKKQFIDSCLNNEKCILHLPEFVFKDNKKLLALDTDASDRI
                     KAVKNFLAVLSDSICSSFIVTSNVASISLGNESFSTDEDLECGYLMNTGNHYDVYLPP
                     ELFAQAYKLNNKERNAQLDYLTRYAI"
     gene            15814..16734
                     /gene="cbpA"
                     /locus_tag="PN977_003221"
     CDS             15814..16734
                     /gene="cbpA"
                     /locus_tag="PN977_003221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curved DNA-binding protein"
                     /protein_id="HDI6043231.1"
                     /translation="MELKDYYAIMGVKPTDDLKTIKTAYRRLARKYHPDVSKEPDAEA
                     RFKEVAEAWEVLSDEQRRAEYDQMWQHRNDPQFSRQFQHGDGQSFNAEDFDDIFSSIF
                     GQHARQSRQRPATRGHDIEIEVAVFLEETLTEHKRTISYNLPVYNAFGMIEQEIPKTL
                     KVKIPAGVGNGQRIRLKGQGTPGENGGPNGDLWLVIHIAPHPLFDIVGQDLEIVVPVS
                     PWEAALGAKVTVPTLKESILLTIPPGSQAGQRLRVKGKGLVSKKQTGDLYAVLKIVMP
                     PKPDENTAALWQQLADAQSSFEPRKDWGKA"
     gene            16734..17039
                     /gene="cbpM"
                     /locus_tag="PN977_003222"
     CDS             16734..17039
                     /gene="cbpM"
                     /locus_tag="PN977_003222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone modulator CbpM"
                     /protein_id="HDI6043232.1"
                     /translation="MANVTVTFTITEFCLHTGISEEELNEIVGLGVVEPREIQETTWV
                     FDDHAAIVVQRAVRLRHELALDWPGIAVALTLMDDIAHLKQENRLLRQRLSRFVAHP"
     gene            complement(17191..17790)
                     /gene="torD"
                     /locus_tag="PN977_003223"
     CDS             complement(17191..17790)
                     /gene="torD"
                     /locus_tag="PN977_003223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone TorD"
                     /protein_id="HDI6043233.1"
                     /translation="MTTLTAQQIACVYAWLAQLFSRELDDEQLTQIASAQMAEWFSLL
                     KSEPPLTAAVNELENRIAALTVRDDARLELAADFCGLFLMTDKQAALPYASAYKQDEQ
                     EIKRLLVEAGMVTSGNFNEPADHLAIYLELLSHLHFSLGEGSVPARRIDSLRQKTLTA
                     LWEWLPEFAARCRQYDSFGFYAALSQLLLVLVECDHQNR"
     gene            complement(17787..20333)
                     /gene="torA"
                     /locus_tag="PN977_003224"
     CDS             complement(17787..20333)
                     /gene="torA"
                     /locus_tag="PN977_003224"
                     /EC_number="1.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine-N-oxide reductase TorA"
                     /protein_id="HDI6043234.1"
                     /translation="MNNNDLFQASRRRFLAQLGGLTVAGMLGPSLLTPRRATAAQAAT
                     EAVISKEGILTGSHWGAIRATVKDGRFVAAKPFELDKYPSKMIAGLPDHVHNAARIRY
                     PMVRVDWLRKRHLSDTSQRGDNRFVRVSWDEALDMFYEELERVQKTHGPSALLTASGW
                     QSTGMFHNASGMLAKAIALHGNSVGTGGDYSTGAAQVILPRVVGSMEVYEQQTSWPLV
                     LQNSKTIVLWGSDLLKNQQANWWCPDHDVYEYYAQLKAKVAAGEIEVISIDPVVTSTH
                     EYLGREHVKHIAVNPQTDVPLQLALAYTLYSENLYDKNFLANYCVGFEQFLPYLLGEK
                     DGQPKDAAWAEKLTGIDAETIRGLARQMAANRTQIIAGWCVQRMQHGEQWAWMIVVLA
                     AMLGQIGLPGGGFGFGWHYNGAGTPGRKGVILSGFSGSTSISPVHDNSDYKGYSSTIP
                     IARFIDAILEPGKVINWNGKSVKLPPLKMCIFAGTNPFHRHQQINRIIEGWRKLETVI
                     AIDNQWTSTCRFADIVLPATTQFERNDLDQYGNHSNRGIIAMKQVVPPQFEARNDFDI
                     FRELCRRFNREEAFTEGLDEMGWLKRIWQEGVQQGKGRGVHLPAFDDFWNNKEYVEFD
                     HPQMFVRHQAFREDPDLEPLGTPSGLIEIYSKTIADMNYDDCQGHPMWFEKIERSHGG
                     PGSQKYPLHLQSVHPDFRLHSQLCESETLRQQYTVAGKEPVFINPQDASARGIRNGDV
                     VRVFNARGQVLAGAVVSDRYAPGVARIHEGAWYDPDKGGEPGALCKYGNPNVLTIDIG
                     TSQLAQATSAHTTLVEIEKYNGTVEQVTAFNGPVEMVAQCEYVPASQVKS"
     gene            complement(20333..>20901)
                     /locus_tag="PN977_003225"
     CDS             complement(20333..>20901)
                     /locus_tag="PN977_003225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309178.1"
                     /note="with TorA forms the inducible trimethylamine
                     N-oxide reductase which transfers electrons from
                     menaquinones first to TorC then to TorA; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="trimethylamine N-oxide reductase cytochrome
                     c-type subunit"
                     /protein_id="HDI6043235.1"
                     /translation="GDTLYSIDIKPIYAAKGDKEASGSLLPASAVKVIKRDGDWLQIE
                     ITGWTESAGRQRVLTQFPGKRIFVASIRGDVQQQVKTLEKTTVADTNTEWSKLQATAW
                     MKKGDMVNDIKPIWAYADSLYNGTCNQCHGAPEISHFDANGWIGTLNGMIGFTSLDKR
                     EERTLLKYLQMNASDTAGKAHGDKKEEK"
BASE COUNT         4885 a         5537 c         5391 g         5088 t
ORIGIN      
        1 tttcaaacag acgcgcgcct gccacaatgc ccgaagcgca ataaatggtg aagaacagca
       61 aaataaccag cgcggagata atgcgcaaaa tgcggctttt atcttcaaag cgcccggtga
      121 aataatccgg caatgttaag gcgttattgt tgtattcggt atgcacacgc aaccgcccgg
      181 ccaccagctt ccagttgatc cacgcgccta atgtcaggcc aatggcgatc cagctttcgg
      241 aaatcccgga aagaaaaaca gcgcctggca accccattaa cagccagccg ctcatatccg
      301 acgcccccgc cgataatgcc gtcacgaatg gcccaagact acgaccgccc agaatatagt
      361 cgtcgaagtt tttcgttgat cgccaggcga taaacccaat caatatcatg ccaaagatat
      421 agacacaaaa tgtcaccaac atcggtgtgc taatagccat ctaaagtctc caaaaaatta
      481 ttatcggcaa tgtcgaaact tgccgttata tctgccaccg gaacggggta acagagttta
      541 tgttttacca gggcgaccgt atcctgccgg aagcgctggt tattcacaat cgatttaaca
      601 caccatttac attaaatttt agtgctcagc gacactattt ttcatcaggt tgcactctct
      661 cacatttttt gcggttgcac ctttcaaaaa tgttaactgc cgcagagaaa aagtctgagt
      721 tattttttta atccctgtca tatcgatttc ttttattaac atttcattca tttttaagct
      781 tgctacgcag gtcacattta acatggttgc acaaagttgc aacatcatgg atatttcacg
      841 ataacgttaa gttgcacctt tcagaacaac aggagtaatg gcatgggaac caccaccatg
      901 ggggttaagc tggacgacgc gacgcgtgag cgtattaagt ctgccgcgac acgtatcgat
      961 cgcacgccac actggttaat taagcaggcg attttttctt atcttgaaca actggaaaac
     1021 agcgatactc tgccggagct acctgcgctg ctttctggcg cggccaatga gagcgatgaa
     1081 gcaccgactc cggcagagga accacaccag ccattcctcg actttgccga gcaaattttg
     1141 ccccagtcgg tttcccgcgc cgcgatcacc gcggcctatc gccgcccgga aaccgaagcg
     1201 gtttcgatgc tgctggaaca agcccgcctg ccgcaaccag ttgctgaaca ggcgcataaa
     1261 ctggcgtatc aactggccga taaactgcgt aatcaaaaaa atgccagtgg tcgcgcaggt
     1321 atggtccagg ggttattgca ggagttttcg ctgtcatcgc aggaaggcgt ggcgctgatg
     1381 tgtctggcag aagcgttgtt gcgtattccc gacaaagcca cccgggacgc gttaattcgc
     1441 gacaaaatca gcaacggtaa ctggcagtca cacattggtc gtagcccgtc tttgtttgtt
     1501 aatgccgcca cctgggggct gctgtttacc ggcaaactgg tttccaccca taacgaagcc
     1561 agtctctccc gctcgctgaa ccgcattatc ggtaagagcg gtgaaccgct gatccgcaaa
     1621 ggtgtggata tggcgatgcg cctgatggga gagcagttcg tcactggcga aaccatcgcg
     1681 gaagcgttag ccaatgcccg caagctggaa gagaaaggtt tccgttactc ttacgatatg
     1741 ctgggcgaag ccgcgctgac tgccgcagat gcacaggcgt atatggtttc ctatcaacag
     1801 gcgattcacg ccattggtaa agcgtccaat ggtcgtggca tctatgaagg tccaggcatt
     1861 tcaatcaagc tgtcggcgct gcatccgcgt tacagccgcg cccagtatga ccgggtaatg
     1921 gaagagcttt acccacgtct gaaatcactc accctgctgg cgcgtcagta cgatattggt
     1981 attaacatcg atgccgaaga ggccgatcgt ctggagatct ccctcgatct gctggaaaaa
     2041 ctctgtttcg agccggaact ggcaggctgg aacggcatcg gttttgttat tcaggcttat
     2101 caaaaacgct gcccgttggt gatcgattac ctgattgatc tcgccacccg cagccgtcgc
     2161 cgtctgatga ttcgcctggt gaaaggcgcg tactgggata gtgaaatcaa gcgtgcacag
     2221 atggacggcc ttgaaggtta tccggtttat acccgcaagg tgtataccga cgtttcttat
     2281 cttgcctgtg cgaaaaaact gctggcggtg ccgaatttaa tctacccgca gttcgcgacg
     2341 cacaacgccc atacgctggc agcgatttat caactggcgg gtcaaaacta ctacccgggt
     2401 cagtacgagt tccagtgcct gcatggtatg ggcgaaccac tgtatgagca ggtcaccggg
     2461 aaagttgccg acggcaaact taaccgtccg tgtcgtattt atgctccggt cggtacacat
     2521 gaaacgctgc tggcgtatct ggtgcgccgc ctgctggaaa acggtgctaa cacctcgttt
     2581 gttaaccgta ttgccgacac ctctttgcca ctggatgaac tggtcgccga tccagtcact
     2641 gctgtagaaa aactggcaca acaggaaggg caaactggat taccgcatcc gaaaattccc
     2701 ctgccgcgcg atctttacgg tcacgggcgc gacaactcgg cagggctgga tctcgctaac
     2761 gaacaccgtc tggcctcgct ctcttcagcc ctgctcaata gtgcactgca aaaatggcag
     2821 tccttgccaa tgctggaaca accggtagcg gcaggtgaga tgtcgcccgt tattaaccct
     2881 gcggaaccga aagatattgt gggctatgtg cgtgaagcca cgccgcgtga agtagaacag
     2941 gcgctggaaa gtgcggttaa taacgcgcca atctggtttg ccacgcctcc ggctgaacgc
     3001 gcggcaattt tgcatcgcgc tgccgtgcta atggaaagcc agatgcagca actgattggt
     3061 attctggtgc gtgaagccgg aaaaaccttc agtaacgcca ttgccgaagt gcgcgaagcg
     3121 gtcgattttc tccactacta tgccggacag gtgcgggatg atttcgctaa cgaaacccac
     3181 cgtccattag ggcctgtggt atgtatcagt ccgtggaact tcccgctggc gattttcacc
     3241 ggacagatcg ctgccgcact ggcggcaggt aacagcgtgc tggcaaaacc ggcagaacaa
     3301 acgccgctga ttgccgcgca agggatcgcc attttgctgg aagcgggtgt accgccaggc
     3361 gtggtgcaat tgctgcccgg tcggggtgaa accgtgggcg cgcaactgac gggtgatgat
     3421 cgcgtgcgcg gggtgatgtt taccggttca accgaagtcg ctacgttact gcagcgcaat
     3481 atcgccagcc gcctggacgc tcagggtcgc cctattccgc tcatcgctga aaccggcggc
     3541 atgaacgcga tgattgtcga ttcttcagca ctgaccgaac aagtcgtaat agatgtgctg
     3601 gcctcggcgt tcgacagtgc aggtcagcgt tgttcggcac tgcgcgtgct gtgcctgcaa
     3661 gatgagattg ccgaccacac gctgaaaatg ctgcgcggcg caatggccga atgccggatg
     3721 ggtaatccgg gtcgcctgac caccgatatc ggtccggtga ttgatagcga agcgaaagcc
     3781 aatatcgagc gtcatattca gaccatgcgt agtaaaggcc gtcaggtgtt ccaggcggtg
     3841 cgggaaaaca gcgaagatac ccgtgaatgg caaagcggca cctttgtcgc tccgacgctg
     3901 atcgaactgg atgactttgc cgaattacaa aaagaggtct ttggtccggt gctgcatgtg
     3961 gtgcgttaca accgtaacca gctgccggcg ctgatcgagc agattaacgc ttccggttat
     4021 ggtctgacgc ttggcgtcca tacgcgcatt gatgaaacca tcgcccaggt cactggctcg
     4081 gcgcatgtcg gtaatctgta cgttaaccgt aatatggtgg gcgcagtggt tggggtgcaa
     4141 ccattcggcg gcgaagggtt gtccggcacc gggccgaaag caggcggtcc gctctatctc
     4201 taccgtctgc tggcgaatcg cccagaaagt gcgctggcag tgacgctcgc gcgtcaggac
     4261 gcagagtatc cggtcgatgc gcagttgaaa gccgcattga ctcagccgct aaatgcactg
     4321 cgggaatggg cggcaaaccg tccagaattg caggcgttat gtacgcaata tggcgagctg
     4381 gcgcaggcag gaacacaacg attgctgcca gggccgacgg gtgaacgcaa cacctggacg
     4441 ctgctgccgc gtgagcgcgt attgtgtatc gcggatgatg aacaggatgc gctgactcag
     4501 ctcgccgccg tgctggcggt gggcagccag gtactgtggc cggatgacac gctgcatcat
     4561 cagttagtga aggcattgcc atcggcagtc agcgaacgta ttcaactggc gaaagcggaa
     4621 aatataaccg ctcaaccctt tgatgcggtg atcttccacg gtgattcgga tcagcttcgc
     4681 gcattgtgtg aagccgttgc cgcgcgggat ggtgcaattg tttcggtgca gggttttgcc
     4741 cgtggcgaaa gcaatatcct tctggaacgg ctgtatatcg agcgttcgct gagtgtgaat
     4801 accgctgccg ctggcggtaa cgccagttta atgacgatcg gttaaacagg tagccggagg
     4861 atgttacaac ctcctccggc atctttaacg tggtcgaatc ccctcaataa taatccgctg
     4921 cacgttttca accgtttgat tgaaaaatac ctcatcgcgc aacgtcgcgc ctgtcaccgc
     4981 ctccacctga ggggcgaaat cggcgtaatg ttgagtggaa gcccaaatca taaaaatcaa
     5041 atgctgcgga tcaatcggcg cgagtttgcc gctttttacc caaccggcaa tcagcgccga
     5101 tttctcatca attaatgcct tcaaatcgcc cgtcagttca tccattaaca acggcgcgcc
     5161 tgccagcatc tccatacaaa acagacgtga agcctgcgga taatcgcgtg agacttccag
     5221 cttcagacgg atgtactctt tgatcgccgc cagcggggcg aaatcttcac gaaacgcttt
     5281 taacggtgcc agccagatat cgagaatctg ccgcagtacg gctatataca gcgcctcttt
     5341 tgacgggaag taatacagca gattggtttt tgaaacgcct gccaactctg cgatctgctc
     5401 cagccttgtg ccgtgaaaac cgaattgtga aaaagtgtcc agtgctgcgc taagaatcgc
     5461 ttttttcttc gcgcttactg cgcgcgaacg tttacccgtt attttcactg cgccttgcgt
     5521 catgcgctct cccctcttgt agattggcaa caggatagca aaacccccgc ccggcctcga
     5581 ccttctgcac tctcatcgcg cacggatgca tgttttatgt gcaactgttt tgaccgttta
     5641 gtccactttt ttccagatat ttcagacaac caaacattaa cacattaata acattcgctt
     5701 tttaaaaact ggcatccgct ttgcaaacaa gccattacgc ggctgaaaag gatatgcagg
     5761 atgcagcgcc ccgtctgact ttcactttac gagacgaaga gaggttgatg atgaaaattg
     5821 gcgtattcgt acctattggc aacaacggct ggctcatttc gacccacgcg ccgcagtaca
     5881 tgccgacctt tgaactgaat aaagccattg tgcaaaaagc ggagcactac catttcgact
     5941 tcgccctgtc gatgatcaaa ctgcgtggct ttggcggcaa aactgagttc tgggatcaca
     6001 accttgagtc gttcaccttg atggcgggtc tggcagccgt gacctcacgc attcagattt
     6061 acgcgacagc tgccacctta acgttacctc cggcaatcgt cgcccgtatg gccgcaacca
     6121 tcgactccat ctctggcggg cgttttggcg tcaacctcgt gactggctgg caaaagcccg
     6181 agtatgagca gatggggatc tggcctggcg atgactattt ctcccgtcgt tacgactatc
     6241 tcaccgaata tgttcaggtg ctgcgcgacc tgtggggctc ggggaaaagc gattttaaag
     6301 gcgatttttt cactatggat gattgtcgcg tcagtccgca accgagtgtc cccatgaaag
     6361 tgatctgcgc cgggcaaagc gacgctggca tggcgttctc cgcccggtat gccgatttta
     6421 acttctgttt cggcaaaggc gtaaatacac ccacggcttt cgccccgacc gctgcgcgga
     6481 tgaaacaggc cgcagagcaa accggacgcg acgttggctc ttatgtgttg tttatggtga
     6541 ttgccgacga aaccgacgat gccgctcgtg caaaatggga acactacaaa gcgggcgcgg
     6601 atgaagaggc cttaagctgg ctaaccgaac aaagtcagaa agatacccgc tccggtacag
     6661 acaccaacgt ccgtcagatg gccgatccca cttcggcggt aaacatcaat atggggacgt
     6721 tagtcggttc ttacgccagt gtcgcgcgca tgttagatga agtcgcaacc gtgcctggtg
     6781 ccgaaggcgt gctgttgacc ttcgatgatt ttctgtcggg aatcgaaaac ttcggcgagc
     6841 gcattcaacc actgatgcag tgccgcgccc atctccctgc gctgactcag gaggtggcat
     6901 gatgacgacc ttaaccgctc gaccggaagc cattaccttc gatccgcagc aaactgcgct
     6961 gatcgtggtg gatatgcaaa acgcttatgc cacgccaggc ggctacttag atctcgccgg
     7021 gtttgatgtc tcaaccactc gcccggtcat tgccaacatt caaaccgccg tgaccgcagc
     7081 gcgagcggca gggatgctga tcatctggtt tcaaaatggc tgggatgcac agtatgtcga
     7141 ggctggcggc cccggctcac cgaattttca taaatcgaac gccctgaaaa ccatgcgtaa
     7201 gcagccgcag ctgcagggga aattgctggc gaaaggctcc tgggattatc aactggtgga
     7261 tgaactggtg ccgcagcctg gcgatattgt gctgccgaag ccgcgctaca gcggtttctt
     7321 caatacgccg ctggacagca ttttgcgcag ccgtggaata cgccatctgg ttttcaccgg
     7381 tatcgctacc aacgtctgcg tcgaatcgac gctacgcgat ggcttttttc tggagtattt
     7441 cggcgtggtg ttggaagacg caacacacca ggcggggccg gaatttgcac agaaagctgc
     7501 gttgttcaat atcgaaacct tttttggctg ggtcagcgac gtcgaaacgt tctgcgacgc
     7561 gctttctccc acgtcctttg ctcgtatcgc ttaaggagtt taacgatgcc aaaatccgta
     7621 attattcccg ctggcagcag cgcaccgctg gcccccttcg ttcccggcac gctggctgat
     7681 ggcgtggtgt atgtctccgg tacgctggct tttgatcaac ataataacgt gctgtttgcc
     7741 gatgacccaa aggcgcaaac ccgccacgtt ctggaaacca tccgcaaggt gatcgagacg
     7801 gcgggtggca caatggcgga tgtgaccttc aacagcatct ttattaccga ctggaaaaat
     7861 tacgccgcga ttaacgaaat ctacgccgag ttttttccgg gtgataaacc ggcgcgattc
     7921 tgcattcagt gcggactggt aaaacctgac gcactggtgg aaatcgccac aattgcgcat
     7981 atcgccaagt gaggccgcga tgaaactttc actctcacct cccccttatg ctgatgcgcc
     8041 cgtagtggtg ttgatttcgg gtcttggcgg tagcggcagt tactggttac cgcaactggc
     8101 ggtgctggag caggagtatc aggtagtctg ttacgaccag cgcggcaccg gcaataatcc
     8161 cgacacgctg gcagaagatt acagtatcgc ccagatggca gcggaactgc atcaggcgct
     8221 ggtagccgca gggattgagc gttacgcggt ggtcggccat gcgctcggtg cgctggtggg
     8281 aatgcagctg gcgctggatt atcccgcgtc ggtaactgtg ctggtcagcg ttaacggctg
     8341 gctacgaata aacgcccata cgcgccgctg ttttcaggtt cgcgagcagt tactacatag
     8401 cggcggcgcg caggcatggg tggaagcgca gccattgttc ctctatcccg ccgactggat
     8461 ggcggcccgc gcacctcgcc tggaagctga agacgcgctg gcactggcgc attttcaggg
     8521 caaaaataat ttactgcgtc gacttaacgc cctcaaacgc gctgacttta gtcgccatgc
     8581 ggatcgcatc cgctgcccgg tgcaaatcat ctgcgccagt gatgatcttc tggtgccaac
     8641 agcatgttcc agtgaacttc atgccgccct gcccgatagc cagaaaatgg tgatgcgcta
     8701 tggcggacac gcctgcaacg tgaccgatcc cgaaacgttt aatgctctgt tactcaacgg
     8761 gcttgccagc ctgttacatc accgtgaagc cgcctgtaag gaattgctat gaacgaagcc
     8821 gttagccctg gtgcgcttag caccctgttc accgatgccc gcactcacaa cggctggcgg
     8881 gagacacccg tcagcgatga gacgttacgg gagctttatg ccctgatgaa atgggggccg
     8941 acatcagcta actgttctcc ggcacggttc gtgtttatcc gcactgcaga aggaaaagaa
     9001 cgtctgcgcc cggcgctttc cagcggcaat ctgcaaaaaa ccctgaccgc gcccgtcacc
     9061 gctatcgtcg cctgggacag tgaattttat gaacggttac cacagctgtt tcctcacggc
     9121 gatgcccgca gttggtttac ctccagccca caacttgccg aagaaacagc gtttcgcaac
     9181 agttccatgc aggcggccta tctgatcgtc gcctgccgtg cgctggggct ggataccggc
     9241 ccgatgtcgg gttttgaccg tcaacacgta gacgacgctt tttttgcagg cagcacgctg
     9301 aagagcaatc tgctgattaa tatcggctat ggcgatagca gcaagcttta tgcgcgcctg
     9361 ccacgtctgt cctttgagga agcctgcggg ctgttgtaag gagcaaacta tgaacattgt
     9421 cgatcaacaa acttttcgcg atgcgatgtc ctgcatgggc gcggcggtca atatcatcac
     9481 cacggacggt ccagccgggc gcgccgggtt caccgccagc gccgtctgca gcgtgaccga
     9541 tacgccgccc acattactgg tgtgcctgaa tcgtggggcg tccgtctggc cggtattcaa
     9601 tgaaaatcga acgctgtgtg taaatacact tagcgccggg caggagccgc tttcaaacct
     9661 ttttggcggc aaaacgccca tggaacaccg ctttgccgcc gcccgctggc agacgggtgt
     9721 gaccggatgc ccgcaactgg aagaagcact ggtttcgttt gactgccgta tcagccaggt
     9781 ggtgagcgtc ggcacccacg acattctgtt ttgcgccatc gaagcgattc atcgtcacgc
     9841 caccccctac gggctggtgt ggtttgatcg cagttatcac gcgctgatgc gccctgcttg
     9901 ttaacctcct taaggagaca gctgatggca atgttcggtt ttcctcactg gcagttaaaa
     9961 tcgacctcta cagagagcgg cgtggtcgcg ccggatgaac gattgccgtt tgctcagacg
    10021 gcaataatgg gcgttcaaca cgcggtggcg atgtttggcg cgacggtgtt aatgcctatt
    10081 ctgatggggc tggatcccaa tctttccatt ttgatgtcgg ggatcggcac gttactgttt
    10141 ttctttatta ccggcgggcg cgtccccagt tatctcggct ccagcgcggc ttttgtcggc
    10201 gtggtgattg ccgcgacggg ctttaacggt cagggcatta acccgaatat cagcattgcg
    10261 ctgggcggga ttatcgcctg tgggctggtt tataccgtca ttggcctggt ggtgatgaaa
    10321 atcggtacgc gctggattga acggctaatg ccgccggtgg tgacgggtgc ggtggtgatg
    10381 gcgattggcc tgaacttagc gcctatcgca gtgaaaagtg tatcggcttc ggcctttgat
    10441 agctggatgg cggtaatgac ggtgctgtgt attgggctgg tagcggtatt tacccgtggc
    10501 atgatccagc ggttattgat tctcgtagga ttaattgtcg cctgtctgct gtatggcgtg
    10561 atgaccaacg ttttagggct gggaaaagcc gtcgatttta ctctcgtcag ccatgctgcc
    10621 tggttcggtc tgccgcattt ttccactcct ggttttaata gccaggcgat gatgttgatt
    10681 gcgcccgtgg cggtgattct ggtggcggaa aacttaggtc atctcaaggc ggtcgccggg
    10741 atgaccggac gcaatatgga tccgtatatg ggacgggcat tcgtcggtga tggtctggca
    10801 acgatgcttt ccggctctgt cggcggtagc ggcgtcacca cctatgctga aaatatcggt
    10861 gtgatggccg tgaccaaagt ctactcgacg ctggtgtttg ttgctgctgc cgtcatcgcc
    10921 atgctgttgg gcttttcgcc gaaatttggc gcgctgatcc ataccattcc ggccgcggtg
    10981 attggtggtg catcaattgt tgtgttcggg ctgattgccg tcgcaggcgc aaggatctgg
    11041 gtacaaaacc gtatcgattt aagccagaac ggtaatttga ttatggtcgc agtaacctta
    11101 gtgctgggcg cgggtgattt tgctctcacg ctgggcggtt ttacgttggg agggatcggt
    11161 acagcaacct ttggcgcgat tttactcaat gcgttattaa gcagaaggtt ggttgacgtt
    11221 ccaccgccag aagttattca tcaggagcca taaacagaaa accgccgggg ttaccggcgg
    11281 tgatggcttt ttgacgagct taagaaaaaa atgcattcgt cattgattag cccggctagt
    11341 tgtcgctttt gccgtgactg ctcttgccac cttttttacc tgcttcagat gcgcgctgcg
    11401 ggtcattctt gaaattaccc ccgctgtgct gtccaccttt tttacctgct tctgatgctc
    11461 tttcgcggtc ttctgcaaaa ttgccggaac cgcctcgatg gtttgccatt actgacctcc
    11521 gtctgggtat agacatcata ttgcatagaa ttaacgcggc gtatggcggc tgccatattt
    11581 tcgctacgtg ggactaagct tagtgaactg acgtgggcag acctgcgttt ggtaatatcc
    11641 tgcaacagct tcttagcgag aatagatatc atctccaata attatccata agccgctctt
    11701 atgattaaaa aaacgcgatg agataaaaat gtgtcatttt gcgacaaaat tacgtgcttg
    11761 tgaaagttgt tataaatcaa ataagtggtt gtgaaatttg cactctgaaa aggacgtctt
    11821 atctttaaat aagtggtagc gaatcgctac ggaatagaga taacacgagg agtggttaga
    11881 aatggctaaa gttctggtgc tttattattc catgtacgga catattgaaa cgatggcacg
    11941 cgcagtcgct gagggtgcaa gcaaagtgga tggcgcagaa gttgtcgtta agcgtgtacc
    12001 ggaaaccatg ccgccgcaat tatttgaaaa agcaggtggt aaaacgcaaa ctgcaccggt
    12061 tgcaaccccg caagaactgg ccgattacga cgccattatt tttggtacac ctacccgctt
    12121 tggcaacatg tccggtcaaa tgcgtacctt cctcgaccag acgggcggcc tgtgggcttc
    12181 cggcgcacta tacggaaaac tggcgagcgt ctttagttcc accggtactg gcggcggtca
    12241 ggaacaaacc atcacatcca cctggacgac ccttgcgcat cacggcatgg tgattgtccc
    12301 cattggctac gcagcgcagg aattatttga cgtttcacag gttcgcggcg gtacgccgta
    12361 cggcgcaacc accatcgcag gcggtgacgg ttcacgccag cccagccagg aagaactgtc
    12421 tattgctcgt tatcaagggg aatatgtcgc aggtctggca gttaaactta acggctaatc
    12481 ttcaacagga ggatacgcat gccaactcaa gaagcgaaag ctcatcacgt cggtgaatgg
    12541 gcatctctgc gcaatacgtc gccggaaata gccgaggcca ttttcgaagt cgccgggtac
    12601 gacgaaaaaa tggcggaaaa gatttgggaa gaaggtagcg atgaagtgtt agtcaaagcc
    12661 tttgctaaga ccgacaaaga ttcgcttttt tggggcgaac agaccatcga acgtaaaaac
    12721 gtttaaccag cgactccccc gtagcgaacg ggggagtttt ctgttatttc accgcttcat
    12781 tcaacacgct atcaaactta tccattgggc agaaaccgtt ggcatctatc gggcaaccgc
    12841 ttaattccag cgtcacgcgc tgcgcaggtg cctgaagggt taacgcgtca gcattacgta
    12901 actgttctgc actctggtac acatactcga ttttcatcag gtcgcgattg gttttgctgt
    12961 catgccaacg ctggaaaacg attttgccgc caatcggcgt gcgttcgttc tggtcatgca
    13021 actgatacgg tttgaaatcc agcgccgtta acagagaggc aatgttggag tcatgcccaa
    13081 ctaacaccgt gattttcgga gcgctggtgc gatcggtgac cagggctttg tcgatataac
    13141 tgaccagcgg tttcgcaaca ttgcgcgcca cttccggtga ggtaaacagg ctgtcctggt
    13201 aaccgttttt cagcttcgac aaccccctcc actgctggtc agatttgatt tctccccagg
    13261 ccacctgatc catcggaaaa ccttcgtagt attgcaaagt aaacgcatct accagcgagt
    13321 tgccgacttt cagcggcccg gaaacgcccg gttcttgttg atacttcgcg ctaaaggtat
    13381 ttttgccatc caccagcgaa cattgttgtt tctctttaca cgcaggggaa tctttatagt
    13441 taacgatttt ttccagtagc tggtagctgt cggtaagctg gagtttgctg agctctttct
    13501 ccattgccgc caccgcctgt tcactgaatg cggcggaatc atcggtgatc accggattaa
    13561 aggttgggtc catagtgccc attttttcct ggtgatgcac aggaatatcg caccccggga
    13621 atgcgccggt aataaagaac tgtgcagtcg caacggtacg ttgcagacta ttggcatagg
    13681 cataaacggt gtccggcggc gggcattccc ccgattttac catcccctgc tctgccagcc
    13741 attcacgcat gtaatggccc atatacactt cgagcacgcc acctttggtg gtgagttgcc
    13801 caccggggac gtcccattca ggccatttat tcggcgtcga ctgctccagc acactgccat
    13861 tgttcgccag cggcgcacgt aagttatggc ggctcatcat gagcacttgc tgtagctgat
    13921 agccttccgg tacggtttgt gcctgagcat ttgaagcgag taaaactatc cctgccacag
    13981 ttgcggcgat tagcgttttg ttcattcctg gcacctcttt tgttatcaat cactaaagag
    14041 tgtgacagaa atatgacttt tttccgggaa caggttcgca aaatggcttg ttgaaaacct
    14101 gtttttctat atacatcttc aaaaacaggc aaggtttgac gcaaactatg cctgattagc
    14161 ccctgcctcg cgtacgccgc tgataattgc acctgtggct aaacagaata tctgcggcgt
    14221 taccaataca gaaacaatat ttaccaataa aaaagtgtga gactaataac aatggtttat
    14281 agcatacgcc tttgtcatta aattgcataa cgagttaaat agtcgagttg cgcattcctt
    14341 tccttattgt ttaacttgta agcctgagca aaaagttcag gaggaaggta aacgtcataa
    14401 tgattgccag tattcattaa ataaccacac tcaaggtctt catctgttga aaatgattcg
    14461 ttccccaagg agattgatgc cacattactg gtaacaataa acgatgagca aatgctatct
    14521 gaaagaacag caagaaaatt cttcaccgct ttaatcctat ccgaagcgtc ggtatctaaa
    14581 gcaagcaact tcttgttatc tttaaataca aactcgggta aatgcaggat acatttttcg
    14641 ttattcaagc aactatcaat gaattgtttt ttaaaaaaca gaaagctcca tgacatatga
    14701 ctacccacac cctgaatttt gttgtcagat tgcagatcaa cgccaaataa tgtcttaaca
    14761 tcttccctga gatcacaaaa tgcagcatca acaagagcct cgattgttgg tttatcaaca
    14821 attttaataa actcttcgta tacttccttt tgcgtcttat gactgcccag cgcccatgaa
    14881 taatcatgtt gttttaaaac cagtatggct cttaacagac aatatccatc gccatctact
    14941 ggagcgtatt ttaattcata agttttatta atttcactct gaataaaaga tataccagac
    15001 aaatcaatac cagtatctaa atcagatatt cccggcgtat ttatctgttt tggctggaca
    15061 cttatatttt catcatctaa acaatactca gaaaaaacat catcacacgc attaatgttt
    15121 tcaatatatt ccataaagcc ataccaataa taacctttaa aatctgtgat agactctgaa
    15181 gctctgcccg gcaaggacac tctaacaatg tcaatatctc gaacatattg tatagtatat
    15241 gtaatacctc ctgaaacccc tgaataaatt tgtgtatcat tagtacgcaa tgttctcata
    15301 aataatgata gatcattatc attctgcatt tcctcaaatt ctttttctag tgattcgcat
    15361 tccaggcatg gaatgtagtc ttcagctttt tgacgtctga tttctttata caaccgcata
    15421 aaacacacca gtgtgttttc aaaaagcgtg attgaatatg tttgattttt ttcgtgctct
    15481 ttttttatat tgtgccaggc ttgtttcaaa taattatttg cagtgcaact aattccatgt
    15541 atattactac ccatatatag cgcctataaa atttaataaa taatgacgca ctagtaaaac
    15601 ttaacgtgcc tggttcaact atcaaaaatc gctcaccctt tttcacctgt ttaaaatatg
    15661 ttcagcaccc caccttgagg cgacctcctc tccgcgatga tttcaataac atattctgtg
    15721 ttggcatatg aaattttgag gattacccta cacttatagg agttacctta caggggttcc
    15781 ttcaatttgt gttgatttac gcgagataac gctatggaat taaaggatta ttacgccatc
    15841 atgggcgtga aaccgacgga cgatctcaag acaatcaaga ccgcctatcg tcgactggcc
    15901 cgcaaatacc atcctgatgt cagcaaagaa ccggatgccg aagcccgctt caaagaggtc
    15961 gctgaagcct gggaagtgtt gagtgatgaa caacgtcgcg ctgagtatga tcagatgtgg
    16021 caacatcgca acgatccgca atttagccgt cagttccagc atggcgacgg tcagagtttt
    16081 aacgccgaag attttgacga tatcttctcg tcaattttcg gtcagcatgc ccgccagagc
    16141 cgtcaacgcc ccgccacacg cggccacgat attgaaatcg aagtggcggt attcctcgaa
    16201 gaaacgctta ctgagcataa gcgtaccatc agctataacc tgccggttta taacgccttt
    16261 ggcatgatcg aacaggaaat tccgaaaacg ctgaaagtga agatcccggc aggggtcggc
    16321 aatggtcaac gtatccgcct gaaaggccag gggacgccgg gcgaaaacgg cggtccaaat
    16381 ggcgatttgt ggctggtgat tcatattgcg ccacatccgc tgtttgatat tgtcggccag
    16441 gatctggaaa ttgtggtgcc ggttagcccg tgggaagcgg cgctgggtgc taaagtcacc
    16501 gttccaacac tgaaagaaag cattttgctg actatcccgc ctggcagcca ggccgggcaa
    16561 cgattgcgcg ttaaaggcaa aggtctggtg agcaaaaaac agaccggcga tctgtatgcg
    16621 gtactgaaaa tcgtgatgcc gccgaaaccg gatgaaaaca ctgccgcgct gtggcagcaa
    16681 ctggcagacg cccagtcgtc ttttgaacca cgtaaagatt gggggaaagc ataatggcta
    16741 atgttacggt gacttttact attaccgaat tttgcctgca taccggcatc tctgaagagg
    16801 agttgaatga aattgtcggt ttgggggtgg ttgaaccgcg tgagatccag gaaacaacct
    16861 gggtatttga cgaccatgcc gccattgtgg tgcaacgtgc ggtacgcctg cgtcatgaac
    16921 tggctctgga ctggccgggg atcgcggtgg cgctgacgtt aatggatgat attgcgcacc
    16981 tgaagcagga aaaccgcctg ctgcgccagc ggctttcccg gtttgtagct catccatgat
    17041 gtgtgtttgc cggatgtgat gacgcatctt aaccggtcca caaagagtac ttttcctgtg
    17101 agtcggttaa ggcgttcacg ccgcatccgg cggcggtgcc ctaatgcctg atgcgacgct
    17161 aaacgcgtct tttcaggcgc acaaacgacg ttatctgttt tggtggtcgc actccactaa
    17221 caccagcaat aactggctta gtgccgcgta aaaaccaaag ctatcatact gacgacaacg
    17281 cgcagcaaac tctggtaacc attcccacag tgccgtcagt gttttttgcc gcaaactgtc
    17341 gattcttcgc gcaggaacgc tcccctctcc cagcgaaaaa tgcagatgac tcaacaactc
    17401 gagatagatc gccagatgat ctgccggttc gttgaaattg ccgctggtta ccatccctgc
    17461 ctcaactaac aagcgtttaa tctcttgctc gtcctgtttg taggccgatg catacggcag
    17521 cgcagcttgt ttgtcggtca tcagaaacag gccgcaaaag tcagcggcca gttccagacg
    17581 ggcatcgtca cgtacggtca gggctgcaat acggttttcc agctcattca ccgccgcagt
    17641 gagcggcggt tcacttttca gcaacgaaaa ccattcagcc atctgcgcac tggcgatttg
    17701 cgtcagttgt tcatcgtcca gttcacggga gaacaactgc gccagccagg cgtaaacaca
    17761 ggcaatctgt tgtgctgtca gcgtggtcat gatttcacct gcgacgcggg aacatattca
    17821 cactgcgcca ccatctccac ggggccgtta aacgccgtta cctgctccac tgttccgttg
    17881 tacttctcaa tttccaccag cgtagtgtgc gcactggtcg cctgcgccaa ctgcgaagta
    17941 ccgatgtcaa tggtcaacac gttgggatta ccgtatttgc acagcgcacc aggctcgccg
    18001 cctttatctg gatcgtacca tgccccttca tgaattcgtg ccacgccggg tgcatagcgg
    18061 tcagaaacca ctgccccagc caacacctga ccgcgagcgt taaagacgcg taccacatca
    18121 ccgttacgaa taccgcgcgc gctggcatcc tgcgggttaa tgaacactgg ctctttaccc
    18181 gctaccgtat attgctgacg cagagtttcc gactcacata actgcgagtg aagtcggaaa
    18241 tccggatgca cagattgcag atgcaacgga tacttctgcg agccgggccc gccgtgggag
    18301 cgttcgattt tctcaaacca catcggatgc ccctgacaat cgtcgtagtt catatcggcg
    18361 atggttttcg agtagatctc aatcaggcca ctcggcgtgc ccagcggttc gagatccgga
    18421 tcttcgcgga atgcctggtg gcgaacaaac atctgcggat ggtcaaactc gacatactct
    18481 ttgttattcc agaagtcatc aaacgctggc agatgaacgc cgcgtccttt gccctgttgt
    18541 acaccttcct gccagatgcg tttcagccag cccatttcgt ccagcccttc ggtaaaggct
    18601 tcttcgcgat taaagcgacg gcacagctcg cggaaaatat caaagtcgtt gcgcgcctca
    18661 aactgcggcg gcacgacctg tttcatggcg ataatgccac ggttagagtg gttaccgtac
    18721 tggtcgagat cgttacgctc aaactgcgtg gtcgcaggca gtacgatatc ggcaaagcgg
    18781 caggttgagg tccactggtt atctatggcg ataaccgttt ccagcttgcg ccagccttca
    18841 ataatgcggt tgatctgctg atggcgatgg aacgggttag ttccggcaaa aatacacatt
    18901 ttcagcggcg gcagttttac cgatttaccg ttccagttga tcactttccc cggttcgagg
    18961 atcgcatcga taaaacgggc aatcggaatg gtgctgctgt aacctttgta atcactgttg
    19021 tcgtgaacag gcgaaatcga cgtagagccg gagaaaccac tcagaataac gcctttacgc
    19081 cccggcgtgc ctgcgccgtt atagtgccag ccaaaaccaa agccaccacc tggcaggcca
    19141 atttgcccca gcatcgccgc cagaacgaca atcatccacg cccactgttc accgtgctgc
    19201 atacgctgta cgcaccagcc agcaataatc tgcgttctgt tcgccgccat ctgccgcgcc
    19261 agcccacgaa tggtttcggc atcaatgccg gtcagttttt cagcccatgc ggcatctttc
    19321 ggctgaccgt ctttctcacc cagcagatac ggcaggaact gctcaaaccc cacacagtag
    19381 ttagcgagga agtttttgtc gtacaggttt tcactgtaca gcgtatacgc cagcgccagt
    19441 tgcagtggca cgtcagtttg cgggttaaca gcaatgtgct tcacatgctc gcgccccaga
    19501 tactcatggg tggatgtgac aaccggatcg atgctgatga cctcaatttc accggcggcg
    19561 actttcgctt tcaactgcgc gtaatattca taaacatcgt gatccgggca ccaccagttc
    19621 gcttgctggt ttttcagcaa atcagagccc cacagcacaa tggttttgct gttctgcaat
    19681 accagcggcc aggaggtttg ctgttcatac acttccattg agccaaccac gcgcggcagg
    19741 atcacctgcg cagcaccggt agagtaatct ccgcccgtac caacgctatt accatgcaag
    19801 gcaatagctt tcgccagcat ccccgaagcg ttatggaaca tccccgtcga ttgccaacca
    19861 ctggcggtca gcaaggcact cggcccgtga gttttctgta cgcgttccag ttcttcatag
    19921 aacatgtcga gggcttcatc ccagctcacg cgcacaaaac ggttatcacc gcgctgggaa
    19981 gtgtcgctca gatggcgctt acgtagccag tccacgcgta ccatcgggta acgaatacgc
    20041 gccgcgttgt gtacgtgatc cggcaatccg gcaatcattt tcgacggata tttatccagt
    20101 tcgaacggtt ttgccgccac aaagcgacca tccttcaccg tcgcgcggat agccccccag
    20161 tgcgacccgg taagaatgcc ctctttcgag atgacagcct cagtcgccgc ttgcgccgca
    20221 gtcgcacggc gcggcgttaa caatgacggc cccagcatcc cggcgacggt taagccgccg
    20281 agttgtgcca gaaaacgccg acgtgatgcc tgaaagagat cgttattgtt cattattttt
    20341 cttccttctt atcgccgtga gccttacctg cggtatcaga cgcattcatt tgcagatatt
    20401 tcaacaaggt gcgttcttca cgtttatcga ggctggtaaa gccaatcatg ccgttgagcg
    20461 tgccgatcca accgttagcg tcaaagtgag agatttccgg tgcgccgtgg cactggttac
    20521 aggtgccgtt gtacaacgaa tccgcataag cccagatcgg tttgatatcg tttaccatgt
    20581 cgcctttctt catccacgca gtggcttgca acttgctcca ctcggtattg gtgtcggcaa
    20641 cggtggtttt ctccagcgtt ttcacctgct gctgcacatc accacgaatc gaggcaacaa
    20701 agatgcgttt acctgggaat tgggtgagta cacgctgacg tcctgcactt tccgtccagc
    20761 cagtgatttc aatttgcagc cagtcgccgt cacgtttaat gactttcact gccgaagcag
    20821 gcagcagaga accagaggct tctttatcgc ctttcgccgc ataaatcggc ttaatatcaa
    20881 tggagtacag cgtgtcacca c
//
LOCUS       DAMZFT010000084        20612 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.084, whole genome shotgun sequence.
ACCESSION   DAMZFT010000084 DAMZFT010000000
VERSION     DAMZFT010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20612)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20612)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20612
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.084"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..1309
                     /locus_tag="PN977_003226"
     CDS             <1..1309
                     /locus_tag="PN977_003226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001368704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HDI6043236.1"
                     /translation="QSSQALQNLQRQLGLIAGGEAGKRAATAVGLRCSADTLLRRVIN
                     TPETKQSGSPHVGIDEWAWHRGHRYGTLIVNLDTHRPLVLLPGRDQRTLATWFRKYPE
                     IQIVSRDRSGVYATAAREGAPQARQVADRWHLLKNIGDAPERMMYRHIPLIRLVASEL
                     SPKKSPDPEPSVPAASLRRPERLKQQTRKKRHQRWTEVMALHNKGCSFREISRITGLS
                     RVTVSRWVRSGTFPEMSTRPPKRGLLDPWREWLKEQRESGNYNASRIWREMVARGFTG
                     SETIVRDAVAKWRKGWNPPVTTAVRLPSVSRVSRWLIPWRITRDEENYASRFISLMCE
                     KEPELKIAQQLALEFYRILKTQNKSQLSSWFTRVHESGSAEFRRVAAGMEADAAAICE
                     AISSHWSNGVVEGHVNRLKMLKRQMYGRAGFELLRQRVMSPLA"
     gene            complement(1454..1676)
                     /locus_tag="PN977_003227"
                     /pseudo
     CDS             complement(1454..1676)
                     /locus_tag="PN977_003227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001610815.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
     gene            1757..1846
                     /locus_tag="PN977_003228"
                     /pseudo
     CDS             1757..1846
                     /locus_tag="PN977_003228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502604.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
     gene            complement(2040..2741)
                     /locus_tag="PN977_003229"
     CDS             complement(2040..2741)
                     /locus_tag="PN977_003229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000466879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6043237.1"
                     /translation="MFGHLLRYFSFCKKDLLVFHPESDYSDNLHKAFSFTKRLGFNEE
                     WNGVTKNKEYDYQDVFSHVCLQSGVTDFSASAGQCLKWSHYFQSYFENILECRVWVTV
                     GQLWKQERFIYNPSVADFQRWSEKGIQPEDFRHHSGFNFHAWLTTENGVIVDVSFMST
                     LSRRLPEHLSEVSGSVIIGTPETVLPEYKYVPMIVGQRIVEKIEKRSFIGFLAHDDID
                     LYTVPAILVPVWKEC"
     gene            complement(2796..2972)
                     /locus_tag="PN977_003230"
     CDS             complement(2796..2972)
                     /locus_tag="PN977_003230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001373358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
                     /protein_id="HDI6043238.1"
                     /translation="MFFGSDYGSERTCCLNGIDPQAYRRHILSVLLKWLMAVKRDFTY
                     ICIKQDFTTSQHSL"
     gene            complement(3087..3494)
                     /locus_tag="PN977_003231"
     CDS             complement(3087..3494)
                     /locus_tag="PN977_003231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001300094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus-type Gp2 protein"
                     /protein_id="HDI6043239.1"
                     /translation="MADFTGELHRLISEYLQCKRRKDQSVEPTPLYYLWSREAVANDT
                     TPYLMVLLLNNDVWCKSREVVVLRNLIEQAWGKVLGEDNSITHLVVYPEVLSLVFDAA
                     NIYNAEPAVLEQTARAYIESHGTIERHLGCSDF"
     gene            complement(4125..4550)
                     /locus_tag="PN977_003232"
     CDS             complement(4125..4550)
                     /locus_tag="PN977_003232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6043240.1"
                     /translation="MNGIKTGLGITPGEHIISADSALSRNIRQCFCLSCHGRLILQTD
                     AQGAWFEHDLHVLSEQQKAACVVLNPEKSHPYIEDMEMFLSPLPVVLEWHCVMCEQFF
                     HGKKFCEACGTGIYCRAVCTKSVYSYQPDLFQDCGGAST"
     gene            complement(4547..4930)
                     /locus_tag="PN977_003233"
     CDS             complement(4547..4930)
                     /locus_tag="PN977_003233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000270955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6043241.1"
                     /translation="MYAKSFIAFDGNGRLTGARTAQTAPYDRYTCHLCGSALRYHPQY
                     DTERPWFEHTDDGLTEHGQQCPYVRPERREIQLIKRLQQFVPDALPVVRKASRHCRQC
                     HHDYYGERYCTHSHTGHFSEEGVAE"
     gene            complement(5302..5901)
                     /locus_tag="PN977_003234"
     CDS             complement(5302..5901)
                     /locus_tag="PN977_003234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus-type Gp2 protein"
                     /protein_id="HDI6043242.1"
                     /translation="MKTYNTNPGYEMDPQLLTHFNQHLDSLFGVYSKLLPFRMDFAYR
                     KNTLSYRCACRYAMCAEILRLINEVGEKLVGYAWVMEYTERKGLHIHFVGYLNGQSHR
                     SSYLVSRLMGDIWRRVTGGDGYYHWCRFNKNYPVNINHVIHYSDHKAVNALRYAISYL
                     AKREQKECGIILKCSGLPEKSNRGRPRLGSPLPETYSLV"
     gene            complement(6133..6282)
                     /locus_tag="PN977_003235"
     CDS             complement(6133..6282)
                     /locus_tag="PN977_003235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004245074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin activation protein"
                     /protein_id="HDI6043243.1"
                     /translation="MEKVISHARYKLTPEEMEAFNSAVDHRLAELTMNKLYDRVPASV
                     WKYVT"
     gene            7318..8109
                     /locus_tag="PN977_003236"
     CDS             7318..8109
                     /locus_tag="PN977_003236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6043244.1"
                     /translation="MTTRITFNMTSDETLRIVDEYCHTHKLSRSKVINTLLNATAPVL
                     NDINCYYQLAGELQSRLLNGVYQRDLPHKRNVVSAEKYCLEIWENKLFTRSVLEFDSS
                     NGVLYALKHKRHYRRDKMIGRVESRYIKDICEYQMQLSGEKTKYACFIYIERTIYNHD
                     NPPDETPVKSAVGNAVILLAKDVIYNEYFFDLRKSFFVSVKDLMASGTKGIPETQKYP
                     DVYCWIPLFSINYGVVITPVYKIDPLKPVTVKKPDKITVVCNYRE"
     gene            8493..8699
                     /locus_tag="PN977_003237"
     CDS             8493..8699
                     /locus_tag="PN977_003237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001564245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family transcriptional regulator"
                     /protein_id="HDI6043245.1"
                     /translation="MPPPVSLMDDQMVDMAFITQLTGLTDKWFYKLIKDGAFPAPIKL
                     GRSSRWLKSEVEAWLQARIAQSRP"
     gene            8787..9395
                     /locus_tag="PN977_003238"
     CDS             8787..9395
                     /locus_tag="PN977_003238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000782722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6043246.1"
                     /translation="MKMKPQYQTRYELLHESYQKWLTGFTRHAVSWGVCHPNIHYFHN
                     LTPGWVSFNGEKPEIAIVPRSLHRLIYGSDKRATPPLDDDLVVNLCTSEHLLVHHPML
                     EGILLSECERLRRRSLANKLISLFRQFGGTELRLKLVWLCWLDLMTGNSLDDWTENLK
                     RKSEKELGEWIIDRQKQNAALADLMDQYVLLAYRTTVDDNRN"
     gene            11104..11320
                     /locus_tag="PN977_003239"
                     /pseudo
     CDS             11104..11320
                     /locus_tag="PN977_003239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001392653.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(11774..12967)
                     /locus_tag="PN977_003240"
     CDS             complement(11774..12967)
                     /locus_tag="PN977_003240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001596162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDI6043247.1"
                     /translation="MSTVSLKQLYGKTHAYWSGVFAMTLCVFVLIASEFMPVSLLTLI
                     AHDLHVTEGLAGQGIAVSGALAVLTSLTISRIVGDLNRKYLLLGLTVLMAISGIIITI
                     APDYPVYMLGRALIGVVIGGFWSMSAATAIRLVPQHQVSRALAIFNGGNALATVVAAP
                     LGSYLGTTIGWRGAFLCLVPLAIAAFIWQWVSLPPMESDKTQKPQRSVLRLFSVAIVP
                     TGLLACGLFFMGQFALFTYVRLFLESVTRVGPSDLSLILLAIGVAGFVGTMIVSTFLN
                     TIFYKTLIMIPLLMAAITGALLLVGHHIWAVAVLLSLWGLLATAAPTGWWTWIARALP
                     EDAETGGGLMVAVIQLSIALGSTAGGLVFDSLGWRSAFGLSGLLLLGAVAMTCATSRQ
                     NRKAQ"
     gene            complement(13145..14281)
                     /locus_tag="PN977_003241"
                     /pseudo
     CDS             complement(13145..14281)
                     /locus_tag="PN977_003241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226527.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
     gene            14452..15336
                     /locus_tag="PN977_003242"
     CDS             14452..15336
                     /locus_tag="PN977_003242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDI6043248.1"
                     /translation="MAKRENYNDLYLFMQVVREGSFTAAAQRLGLAQSGISRAVRELE
                     ERLGVQLLVRTTRKLSLTHAGELLYRNVESGFDALDMGLATLAHYRHTPSGTVRINAS
                     QHAIDKVLLPKLAVFKQRYPDIRLELISESRFVDIITERFDAGIRLGSEVDSGMVAVR
                     ISPDMEMAVVGTPGHFQRYGFPQTPTDLVGHPCIAYQFGDGSLYAWELNQDGKKITHQ
                     PQGQWAFADSYMEAKAARLGLGLAYVPEELVSDDIEQGALIRVLQCYSQRLEGSYIYY
                     PHRNVSPALRAVIDTLRI"
     gene            15566..16438
                     /locus_tag="PN977_003243"
     CDS             15566..16438
                     /locus_tag="PN977_003243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase family protein"
                     /protein_id="HDI6043249.1"
                     /translation="MNPSDAIEAIEKPLSSLPYSLSRHILEHLRKLTSHEPVIGIMGK
                     SGAGKSSLCNALFQGEVTPVSDVHAGTREVQRFRLSGHGHSMIITDLPGVGESRDRDA
                     EYEALYRDILPELDLVLWLIKADDRALSVDEYFWRHILQCGHQQVLFVVTQADKTEPC
                     HEWDMAGIQPSPAQVQNIREKTEAVFRLFRPVHPVVAVSARTGWELDTLVSALMTALP
                     DHAASPLMTRLQDELRTESVRGQAREQFTGAVDRIFDTVESVCVASVARTVLRAVRDT
                     VVSVARAVWNWIFF"
     gene            16810..19656
                     /gene="ag43"
                     /locus_tag="PN977_003244"
     CDS             16810..19656
                     /gene="ag43"
                     /locus_tag="PN977_003244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter adhesin Ag43"
                     /protein_id="HDI6043250.1"
                     /translation="MKRHLNTSYRLVWNHITGTLVVASELARSRGKRAGVAVALSLAA
                     VTSLPVLAADTVVQAGETVSGGALENHDNQIVFGTANGMTISTGLEYGPDNEANTGGQ
                     WIQNGGIANNTTVTGGGLQRVNAGGSVSDTVIRAGGGQSLQGQAVNTTLNGGEQWVHE
                     GGIATGTVINEKGWQAVKSGAMATDTVVNTGAEGGPDAENGDTGQTVYGDAVRTTINK
                     NGRQIVAAEGTANTTVVYAGGDQTVHGHALDTTLNGGYQYVHNGGTASDTVVNSDGWQ
                     IIKEGGLADFTTVNQKGKLQVNAGGTATNVTLKQGGALVTSTAATVTGSNRLGNFTVE
                     NGKADGVVLESGGRLDVLEGHSAQKTRVDDGGTLAVSAGGKATGVTMTSGGALIADSG
                     ATVEGTNASGRFSIDGISGQASGLLLENGGSFTVNAGGQAGNTTVGHRGTLTLAAGGS
                     LSGRTQLSKGASMVLNGDVVSTGDIVNAGEIHFDTQMTQDAVLSRAVAKGASPVTFHK
                     LTTSNLTGQGGTINMRVRLDGSNASDQLVINGGQATGKTWLAFTNVGNSNLGVATSGQ
                     GIRVVDAQNGATTEEGAFALSRPLQAGAFNYTLNRDSDEDWYLRSENAYRAEVPLYAS
                     MLTQAMDYDRILAGSRSHQTGVNGENNSIRLSIQGGHLGHDNNGGIARGATPESSGSY
                     GLVRLEGDLLRTEVAGMSLTTGVYGAAGHSSVDVKDDDGSRAGTVRDDAGSLGGYLNL
                     VHTSSGLWADIVAQGTRHSMKASSDNNDFRARGWGWLGSLETGLPFSITDNLMLEPQL
                     QYTWQGLSLDDGQDNAGYVKFGHGSAQHVRAGFRLGSHNDMNFGKGTSSRDTLRDSAK
                     HSVRELQVNWWVQPSVIRTFSSRGDMSMGTAAAGSNMTFSPSQNGTSLDLQAGLEARV
                     RENITLGVQAGYAHSVSGSSAEGYNGQATLNVTF"
     gene            19984..>20612
                     /locus_tag="PN977_003245"
     CDS             19984..>20612
                     /locus_tag="PN977_003245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF945 domain-containing protein"
                     /protein_id="HDI6043251.1"
                     /translation="MRLASRFGHVNQIRRDRPLTHEELMHYVPSIFGEDRHTSRSERY
                     AYIPTITVLESLQREGFQPFFACQTRVRDQSRRGYTKHMLRLRRDGERNGQHVPEIIL
                     LNSHDGTSSYQMLPGYFRFVCQNGCVCGLSLGEVRVPHRGDVVEKVIEGAYEVVGVFD
                     RIEEKRDAMQSLVLPPPARQALAQAALTYRYGDEHQPVTTADILTPRRR"
BASE COUNT         5447 a         5152 c         5158 g         4855 t
ORIGIN      
        1 acagtcttca caggcgttac aaaatttaca acgtcaactg ggattaatcg ccggaggtga
       61 ggccgggaaa cgggcagcaa cggcagtggg tctccgttgc agtgctgata ctctccttcg
      121 cagggttatc aataccccgg agacgaaaca gtcaggctcg cctcatgtcg gtattgatga
      181 gtgggcgtgg catcggggcc accgttacgg tacgttaatc gtcaatcttg atactcaccg
      241 tcccctcgtc ctgcttcccg gccgtgatca gcgtacgctg gcgacctggt tcagaaaata
      301 tccggaaata cagattgtct cacgtgatcg cagtggagtc tatgcgacag cagcacgtga
      361 aggtgcacct caggccagac aggtggccga tcgatggcat ctgctaaaaa atattggcga
      421 tgcgcctgaa cgaatgatgt atagacatat acctctgata cgtcttgttg ccagtgagtt
      481 gtcaccaaag aaatcacctg atccagaacc atctgtgcct gcagcatcgc tccgtcgtcc
      541 ggaacgccta aaacaacaaa cccgcaaaaa acggcatcag cgttggacag aggttatggc
      601 cctgcataac aagggatgta gtttcaggga aatatcccgt attacaggcc tgtcgcgtgt
      661 gacagtcagt cgctgggtgc gttcaggaac attccctgaa atgtcaaccc gaccgccaaa
      721 gcgagggctt ctggacccat ggagggagtg gttaaaagag caacgagaaa gtggtaatta
      781 taacgccagc cggatatggc gggaaatggt ggcccgggga tttacaggca gtgaaaccat
      841 cgtcagggat gcggttgcca aatggcgtaa aggctggaac ccaccggtta ctactgccgt
      901 cagacttcct tcagtgtccc gggtaagccg ctggttgata ccctggagaa taaccaggga
      961 tgaagaaaat tatgcttccc gatttattag tctgatgtgt gaaaaagaac ctgagctgaa
     1021 aatagcgcag caactggcac tcgagttcta ccgtattctg aaaacccaaa ataaatcaca
     1081 gctaagcagt tggttcactc gagtccacga aagcggttca gcagaatttc ggcgcgtggc
     1141 ggcggggatg gaagctgatg ctgcagctat atgtgaggca atcagtagtc actggagtaa
     1201 tggtgttgtc gaaggtcatg taaatcgcct gaagatgctg aaacgccaga tgtatggtcg
     1261 agcaggattt gaactactca ggcagagggt aatgagtcca ctggcatgaa aatctccacc
     1321 caggttgcgg aagagccact ttcatccgtc gttgacacat gcatcacaac agctcctcat
     1381 aggcactcag tggtcgatgt tccacctgac tgatctgctg ccagagcggg gcgtgaggtt
     1441 ccggtactat ctgctagcca gacgacactg agcagaggcg atgtgaggcc accagtttct
     1501 cattactgta gatccgcaac tcatcgttca gcgatattcg tatcgagact ggctgaccac
     1561 acagcatttc gggaacgctg tactgattac ctccaacttc gttatagcgg gtcccaggac
     1621 acatggcgga tgtcgaagta actggtatcg aagtctgtat ccggtaacgg ctgcagaagc
     1681 acacatcagg aaacgcagtc tcctgattca gtgctaccac atgaaaattt agttaattgt
     1741 taccgtacta aaatttatgt cagaagatgt tctattatcg aaagtcaatc agcgatacaa
     1801 agacgatcat ggtgcgcttg tcacagtgac cagtgttgag gaaacccatg cggggtggct
     1861 atcctcatta ccgtcttata tcactggcgc tgacaatatc cttccatgaa catatacttc
     1921 tgataaatga ctccggttta gtggcaacca agtgcagaca ctgaaagact gctctgttga
     1981 ccgctcaggt aacaagctga gttccggaaa gcggtaagtg catagtgtta ttccgtgctt
     2041 taacattctt tccataccgg aaccaagatt gcaggtactg tatataaatc aatatcgtcg
     2101 tgcgccagaa aaccaatgaa agagcgtttt tctattttct caacaatcct ctgaccgaca
     2161 atcatgggaa catatttata ctcaggaaga accgtctctg gcgtgccaat aatcacactt
     2221 ccactgactt cactcaggtg ttcaggtaat cgccgcgata acgtactcat aaaggaaaca
     2281 tccacaatca caccattttc cgtcgtcaac cacgcatgaa agttaaaccc cgagtgatgt
     2341 ctaaaatctt caggctgaat ccctttttca gaccagcgct gaaaatccgc aactgacgga
     2401 ttgtaaataa atcgttcctg tttccagagt tgtccaacgg taacccatac ccgacattca
     2461 aggatattct caaaatatga ctgaaaataa tgactccact ttaagcattg tcctgctgaa
     2521 gcggaaaagt ctgtcacacc agactgcaga cagacatgac tgaatacatc ctgataatca
     2581 tattctttgt tttttgtgac cccattccat tcttcattaa atcctagcct ttttgtaaaa
     2641 gagaaggctt tatgtaaatt atctgagtaa tcagattcag gatgaaatac taataaatct
     2701 tttttacaga aactaaagta tcgtaacaga tggccaaaca ttaaatgtct ccccaataca
     2761 taaccctata tgtcagatac tctgaacctg aagaattata aagaatgttg actagtcgta
     2821 aaatcctgct ttatgcaaat atacgtaaag tctctcttta ctgccattag ccatttcagt
     2881 agtacgctca gaatatggcg ccgatacgct tgcggatcga tgccattcag acagcaggtc
     2941 cgttcactac cgtaatcgct accgaagaac acattgattg cccctcaata atcctgctgt
     3001 taacatcaaa attgttggtc gggctcgatt tatttaattg tgtaaacagg gcctctcagc
     3061 gaaaggccct tatgcatcac ctgacgttaa aagtcactac acccaagatg acgttcaatg
     3121 gtaccatgtg attcaatgta agcccgggcc gtctgttcca gtacagcagg ctcagcgttg
     3181 tatatgttag ctgcatcaaa tactaacgac agcacttcag gatacacaac cagatgtgta
     3241 atggagttat cttcacccaa tacttttccc cacgcctgct caatcagatt tctgagaacc
     3301 accacctcac gactcttaca ccagacatcg ttattaagta gcagcaccat aagataagga
     3361 gtggtatcgt tagccacagc ttccctactc cagagataat ataatggggt gggctcaaca
     3421 gattgatctt tacgtcgctt acactgcaaa tattcagaaa tgagtctatg cagttcacca
     3481 gtaaaatcag ccatcagaga gggaatggcc ttattaatac cctgccctgg tattaattta
     3541 aattgtaata atttaatttc aggatgtgtg gctgcagccc gatacagagt tgcaaggaca
     3601 cacttttgcc agagggcgtt acaggaaagc ttaacatttg attctgtata cataataaat
     3661 caccttacag ttacaacagg tcaaaaaccg ctgtagccag agttacgctg gcctgatgct
     3721 ttagtaccgg gcatcgtctg ataatccaga cgctccaata agcgctgata ctgctcaggg
     3781 aaatcaggat catgaatatc ctggatgtca cgtccattag cagggaaata aacaatgcag
     3841 ccccctggat taactatgca gaaatcgtcc tgaggcactg ataaatacgg agaggactct
     3901 cgcgtatggt tggttgacac cacagtgcgg attcggcgaa tccgcgatca cggtgcgatt
     3961 tcgcaccaca gtacacaatc aataccccgg gttttattca ggtaagcagg actgcggata
     4021 tccggtgtcg cgcctttgtg tcacgaacgg ggtgggtgcg aaacaccaga taaaatgcag
     4081 gttccgatac ttctgaacgc cctgagcagg tcgggcattt cggattaagt actcgcacct
     4141 ccgcagtcct gaaacaagtc tggctggtag ctgtaaacag acttcgtaca taccgctctg
     4201 caataaatcc ccgtaccaca ggcctcacag aatttcttcc catgaaaaaa ctgctcacac
     4261 atcacacaat gccactccag cacgaccggt aatggcgata aaaacatctc catatcctca
     4321 atgtaagggt gggacttttc cggattcagc accacgcagg ctgccttctg ttgctcgctc
     4381 aggacatgta aatcgtgctc aaaccacgcc ccctgagcat ctgtctgcaa aatcaaccgg
     4441 ccatgacagg aaaggcagaa acactgccgg atatttctgc tgagcgcaga atcagcactg
     4501 ataatgtgtt cacccggcgt gatccccagc cccgttttta taccgttcat tctgccaccc
     4561 cctcctcact gaagtgcccg gtatggctgt gcgtgcagta ccgctcccca taataatcgt
     4621 gatgacattg tctgcagtgc cggctggctt tacgcaccac gggtaaggca tccggtacga
     4681 attgctgcag acgcttaatc agttgtatct ccctgcgttc cggtctgaca taagggcact
     4741 gctgaccgtg ctccgtcagc ccgtcgtcag tgtgttcaaa ccagggacgc tcagtgtcgt
     4801 attgcgggtg gtatctgagc gcactgccgc aaaggtggca agtgtagcga tcgtaaggtg
     4861 cagtctgtgc ggtacgggca cccgtcagac gcccgttgcc atcaaatgca ataaaggatt
     4921 ttgcgtacat cgtcatattc ctcagcagcc agacgacacg caggcaacag ctgtcccggt
     4981 acgggcagtg tggtcagggt gtgaatggcg gttcagttaa aatctggact ggagtttggg
     5041 taaagcaggc tggttgctgt tacagcactg tggttgtcac acaatggccc tccggcgtaa
     5101 aaaactgtca gcaggcaggg atggacatcg gaggatgtgc tgtatcggtt gccggaagga
     5161 atggtaaaga atgtgccatg gttaaatctc cgtatggttc atacggaata gccatgtcgt
     5221 aaaaaatgcg cagagtccct gaagtggcct ccggaaccga ctgtaacaat tagcgccagg
     5281 ccgtagttgt cgttcagggt cttacaccag agaataagtc tccggcaggg ggctgcccag
     5341 tcgcggtcga ccccgattac ttttttcagg aagcccgctg catttcagga tgatgccaca
     5401 ttctttctgt tctcgtttgg ccaggtagct gatggcatag cgtaaggcat tcaccgcttt
     5461 gtgatctgaa tagtgaatga cgtggttaat gtttaccgga taatttttat taaaccggca
     5521 ccagtggtag taaccatccc ccccggtaac ccgcctccag atatctccca tcaatctgga
     5581 taccagatac gaactcctgt ggctctgacc gttcagatag cccacaaaat gaatgtgaag
     5641 ccctttacgt tccgtatatt ccattaccca ggcatacccg accagtttct ctcccacttc
     5701 gttaatcagt cgcagtatct ccgcgcacat ggcgtacctg caggcacatc tgtagctgag
     5761 ggtgtttttt cggtaagcaa aatccattct gaacggtaac agtttcgaat ataccccgaa
     5821 taatgaatcc agatgctggt taaagtgtgt caggagctgg ggatccattt cgtaaccggg
     5881 attggtgttg taagtcttca tgtttcttcc tcgtgttctg taacacagga agaagcgctg
     5941 gcgtaatttt tgaagaaaac gttatcacac gaagacgtgc aggttcacaa ctccccgggc
     6001 gccacagaga aagtgggacc acacccgggt atcagaacca tacagagaac ggcatacgtg
     6061 acagtcaatc acagcgcaga taatatctga agtgatgtca ttgtgacgac tgtacttcat
     6121 cagaaacatt tatcaggtaa catatttcca gactgaagcg ggaacacgat cgtaaagttt
     6181 gttcatggtc agttctgcca gccggtgatc gaccgcagag ttgaaggctt ccatctcctc
     6241 tggagtaagc ttataacgcg cgtgggaaat cactttttcc agtgaacaac agtggaacta
     6301 ctctggttta aaattccatt accctctcgt tatcttgtac tgtgattaac gtaatatcgt
     6361 cataatggcc tgacgttatc ccgaaagcct ttccacttcc cagtttctcc ggtaaaagat
     6421 gtttttaata ctgaaggcat aacgccataa ccagatatta caattaacgt agctttccgc
     6481 aacgtactac atgagtaaca gaggtacagg gttaaacaac actccggaag actttttaat
     6541 gacatgaggc atactctttt acgtattact accaaaactc atcaggtgag ctgcaggaac
     6601 agatcattaa cagtaattcc ttaatcaccg ttatccacag tatcaacact ggcgaagtca
     6661 tactcttagc tataaaggtg atgacgggtt cagaatagtt attacaccat acaacaaaat
     6721 acagaaaaca acaggaaaaa taacagaaaa aaaatagcag acattactta acaaagcacg
     6781 ttacagtcag tgtgtgaaca ttacagattt atattcaggt gttgcgggac tacattactg
     6841 aagggagagt taagaacaga gttacaagta ctactacaga gaatatcact gcacagatac
     6901 ttctatgaga cagacttcta caggacttgg ttacaaggtg tatttacaag gtagaattac
     6961 agggttcatt tacacggtag aattacataa acatacttct ataaataata ctaattaacc
     7021 tttattattt tttaatggta catgaactgt atgtagctgt aacgataaac aaatcaccca
     7081 aaacgaaaaa tactggttat catggttcag agaactcata cgagtggcag cttccccctg
     7141 agcaatgctt cattttcggt ggttctgtgt gatagattta tgctgactac agtggcagta
     7201 atactgcttt ccaccacacg aggataaaac cagtcaaaaa atcaattata tgaattaaca
     7261 gtatttttta tgaaatgtta tgcatatcaa ccagtatgat taacagaaaa cttcatcatg
     7321 acaacacgta taactttcaa tatgaccagt gatgagacac tgcgaattgt ggatgagtac
     7381 tgtcataccc acaaactttc gcgctctaag gtaatcaaca cattactcaa cgccacagca
     7441 cctgtcctta atgacattaa ctgttactat caacttgcag gcgaactgca gtcacgctta
     7501 ttaaatggag tgtatcaacg ggatcttccc cacaaacgca atgtcgtcag tgcagagaag
     7561 tattgtctgg agatctggga aaacaaactt ttcaccagaa gtgtcctgga gtttgacagt
     7621 tcaaatggtg ttctgtacgc gctgaaacat aaaagacact atcgccggga taagatgatt
     7681 ggaagggttg aaagtcggta tatcaaagac atttgcgaat accagatgca actatccggg
     7741 gagaagacga aatatgcctg ttttatttat atagaaagaa caatatacaa tcacgataat
     7801 ccacctgatg aaacacctgt aaaatcggct gttggtaatg cagtcatcct gctggcaaag
     7861 gatgtgatat ataacgagta cttttttgat ctgcggaagt catttttcgt gagcgttaaa
     7921 gatctgatgg cctctggcac aaaaggtatt ccggagacac agaaatatcc tgacgtttat
     7981 tgctggatcc cgcttttctc cataaattac ggcgtggtca ttaccccggt ctataaaatt
     8041 gatcccctga aacccgtaac ggtaaaaaaa ccggataaga tcactgttgt ttgtaattac
     8101 agagaataat ctcagtcttc tccgctcacc acaacagtgt ttacctcgct ttatcttccg
     8161 ttcagtcaat acaaaccacc gctctggcag gtacagggca tggtttctct ccggtactcc
     8221 cctgcgcagt aataatcatc actgaaaata ctgattccgt cacgacaaaa taccatccca
     8281 tattccggca acacaaaatc atcttccgtt tataccggct tcactgacaa aacagacaac
     8341 ctgtattgcc tgtacacaac cgtttctctc ctggcaggaa tattccccta ctgatttttt
     8401 acgtttctcc ccggttgtta tgccggtgaa ggtggtgcgt tgttttcatc atcaacgccg
     8461 gttgagatta acaacatccg gaggagattt ttatgccccc cccagtttcg ctgatggatg
     8521 accagatggt cgatatggcg tttatcactc agctgaccgg cctgaccgat aagtggtttt
     8581 acaagctcat caaggatggg gcctttccgg ctcccatcaa acttggccgc agctcccgct
     8641 ggctgaaaag tgaagtggaa gcctggctgc aggcgcgtat tgcacagtcc cgtccgtaat
     8701 ttttaaccac tgccccttca cccacagcag acacaatctc cggcctgccg gcggcgttct
     8761 gctgcctgtt atcccccgtg aggaatatga aaatgaaacc acagtatcag acccgttacg
     8821 agctgctcca cgaaagctac cagaaatggc tgaccggctt cacccggcac gccgtatcct
     8881 ggggcgtgtg tcatccgaat atccactact tccataatct gacgcccggg tgggtgtcat
     8941 tcaatggtga aaaaccggag attgccatcg tcccccggag tctgcaccgg ctgatttatg
     9001 gctctgacaa acgggccacg ccgcccctgg atgatgatct ggtggtgaac ttatgcacca
     9061 gcgaacatct gctggttcat catccgatgc tggaaggcat tctgctgtct gaatgtgaac
     9121 gtctgaggcg gcgttctctg gcaaataaac tgatcagcct cttccgtcag tttggtggca
     9181 ctgagttgcg cctcaaactg gtctggcttt gctggcttga tttaatgacc ggaaacagcc
     9241 ttgacgactg gacggagaac ctgaaacgta aatcagaaaa agagctgggg gaatggatca
     9301 tcgaccgtca gaagcagaac gcagcactgg cggatctgat ggatcagtac gtgctcctgg
     9361 cataccgcac aacggttgac gataaccgta actgatgccg gttgcttccg gaaggtgcgg
     9421 caaaatacgg gaatgctgct cccggccccg catgctcacc tgcagagcgg gggcgtcgtc
     9481 atactgagcc atgacggatg tcttaagggc atcctgtcac tgctgagctg gcatgctgcg
     9541 gtacatgcct ctcctggtga caataagtac atggcgacag cgctccctga accgcggaag
     9601 agatgtacac ggccggacgc cgtcagtgta tacgggtaga cgttccatgc tgcatgacta
     9661 catccggccc ggatatcaat gagtccgtca ggaacgcttc acttcgaaac ccggcctgga
     9721 taccagcgcc ggcatgccgg gaagaagttg atatggtggt tgcagggcaa cagcaatcct
     9781 gaatcaactg ctgcatcact caaccacgct gaatatcaaa ggagagcgtg accagttaaa
     9841 agagaaacgc aaatctgtag tgctgcacaa aaatacaaca tcagtcagtg aggatgaaat
     9901 ggtgaaaagc agacagcatc aatattgaaa atatcctggc tggcatgaaa atggcccggc
     9961 ccgtggcgtt gacatattct ctcctgagca tcctgaatga ctgcagttac tgtggaaaag
    10021 taagcagtct ccacccacgg caccacttaa cagacattca gtaactcccc ccggactttc
    10081 tatgatgcat tatcggtgca ctgaatacag gagagtgcgc ttaaaaccgc agaccgcatt
    10141 gcgggtgtta ctgcatgact gcttggcggt attgtgagat gtcggcagga accgatgtct
    10201 gcgcaggggg aacttccgtg atcgggataa gtgcagaaga aatcagatca cgacgtaaac
    10261 ctgcagtgcc tgacgctgca agtggcatta accatgggac gttgccgatc ccggattgcg
    10321 gaaacaggag cactattttc tgccgcgggg agtacctctg gcagtaaatc cggctcgctc
    10381 atctgaccgt ctctctcccg gaaaagacca gaaatgagat cgtcacctca acagcaggga
    10441 agatgtcttg acagactggt tatattgcat acgatttatg aagaaaaaga cagcaaatat
    10501 ggaggctata gcggagaaca ccctacgaaa ttcctgtgaa aaacgattga aaaaacgatc
    10561 agattcgatt cgtttttata tgcttttggg cacgtggcga tgacgaactg ttgcatgtgc
    10621 agaggagaat gcacccggaa gcaccagaat catccagaaa ggcagaacga ttacgcatca
    10681 aaccaggaga cagcaatatc aggataacat actgatttaa aatgaattta cagcaatacg
    10741 tgaaaacatt ttttacttaa aggagacaga tgcgctgaag acagtgcagg tatttttata
    10801 taaaaatgag aaggttacgg gatgtctatc aggcacacta tgaccggaga atatcagtca
    10861 ggcttaaaaa gacaataaat acagtgagtt aaatacacag agaaaacgaa aacgtagtat
    10921 cagagggtac atcttacaac agagactgaa tttccggttt ttcataaccg gcacattccg
    10981 gaaatagcag gctaaccgga ggttttctga caccagtccc tggcagttta atctgtacat
    11041 catgtacgga tgttttgttc gcatttttaa aatcggcaaa aatgcgagaa gagcagctat
    11101 attgtggatg aactgaaaag tgccagggag caacaaaaaa ggcgtgatga acgtgctgat
    11161 ggttccgtcc cgctgaaact gtccggaaaa atccacaaac agcctgaaag tattgcccga
    11221 agtcgtggag cccccccccc caaaaaaact gctgaatgaa atgattgagc agacgtacca
    11281 ggattttgtg gcaaatgagc agcacaagac atggagttaa agactgcagg gccccggtta
    11341 ccaattctgc agcatacaca acgaatgtat ccggctctta cctgtgaggg gcacgaaagt
    11401 tatacattga gcatgagtgg ataatctcgg gtgagagtct cccgttttta cactattcac
    11461 taaaggtgag gcatatcatt cagtctctct gaattcttca tttcagtaac ttcagctacc
    11521 ttcgtttcat ctcccccacg aaccggggcc gcaacttgtt gtatcaatac caaattgttg
    11581 gtcattactg tcctgtatca ctgggctggc aagtgaactg aaatccgcac cttaatattc
    11641 tgactggctg cgtacattca tggcatgaag cggccggagc ggctgagctg aaggtccgtt
    11701 gtgagcgagg agtgatcatt agcctctcag ccggatgcct taaagagcgt acgccagaaa
    11761 taaaaggatt gcgctactgc gccttcctgt tttgacggga tgtggcacat gtcatcgcta
    11821 ccgctcccag aagtaacaaa ccacttaaac caaaagcact tctccatccc agactgtcga
    11881 ataccagacc tccagccgtt gaaccaagtg caatagataa ctgaatcact gcaaccatga
    11941 ggcctccccc cgtctctgca tcttcgggta acgcgcgtgc tatccatgtc caccaccccg
    12001 ttggcgcagc tgtcgcaagt agcccccaca ggctgagtaa aacggcaaca gcccagatat
    12061 ggtgtccgac cagcagtaac gcaccggtaa tcgctgccat aagcaacggg atcattatta
    12121 aggtcttata aaatatggtg ttcaggaatg ttgagacgat cattgtgccg acaaagcctg
    12181 ctacacctat tgcgagcaaa atcagagaaa gatcagaagg tcccactctg gtgacgctct
    12241 ccagaaagag gcgcacgtag gtaaataaag caaactgtcc cataaagaag agtccgcagg
    12301 ccagcagtcc cgttggtacg atggcaacgc tgaacaggcg aagcacagat ctctgtggct
    12361 tttgtgtttt atcactttcc atcggaggca gactgaccca ttgccagatg aacgcagcta
    12421 tcgcaagggg gaccagacat aaaaaggccc cacgccagcc gatggttgtc cccagatagc
    12481 taccaagtgg cgctgccaca acggtggcca gtgcgtttcc gccgttaaaa attgccagtg
    12541 cgcgggaaac ctggtgctgc ggcacgagcc gaatggcggt tgcagccgac attgaccaga
    12601 accctccaat gacaacacca atcagagccc tccccagcat ataaaccgga tagtcaggtg
    12661 ctatagtgat aattattccc gatatggcca ttaagaccgt cagtcccagc aaaagatatt
    12721 ttcgattcag gtctccgaca atacgggaga tagtcagact ggtaagcacc gccagcgcac
    12781 cagagacagc aattccttgc cctgccagcc cttcggtaac gtgcaaatca tgggcaatga
    12841 gcgtaagcag gctgactggc ataaactcag aagcgatcag cacaaacacg caaagcgtca
    12901 tggcgaatac gccgctccag tatgcatgcg tttttccgta taattgtttg agacttacag
    12961 tggacattat tagcccccct taatatcgaa ccagccagat tcaagcgcaa tcgggtcaga
    13021 agttattcag ggaataacga cataatagtt gggtgagaat atagggcgat gcggcatatt
    13081 ctgcatcaca ccaggcagtt aaaagccacc cgtttacgac aggtggcctg gtataccttt
    13141 cgggttatct taagtttgcc ttgaaaaatt gctcaaattt ggcaaatggg atcttacccg
    13201 caacgttgtc gtacagatca acatggtttg cctcggggac aatcactaac tctttctctt
    13261 tgctgccgat tgccttaaag gcatcttcag caaaatagcg tgaatgggct ttttcaccgg
    13321 tcacgatgag tgtagggata gtaacttcat tagcgtaact cagcagcggc atattcataa
    13381 atgacagagg catggttgca ttccaggcac tggttgagtt gaccgaacgc gcatggaaac
    13441 cacgcggcat gcggtaataa tcaaagaact ctttcaatac cggattagaa ttcgctggca
    13501 aagtttccgg taaaatacga tcggaagcgg tgacgttgcc cttctcatca acgtaaatat
    13561 catgcccgcc tggcgcgaaa gcgccatttt cagcatcctt ccagcgctgc atgttcaaat
    13621 actgcaatac ggcacgacga tcggcagtgg tatagcggtc tttgccatcg cccacgccat
    13681 gccccattgc acggctcatg tcatacatca cgctgctcgc caccgccttg acacgtgtgt
    13741 ccatcgcggc gtcgtttaag gccatgccac cccatccgca gatgccgagc aacccgatac
    13801 ggttgcgatc cacctctttt tgcaggccta agaaatcaac cgccgcgctg aaatcctcgg
    13861 tgttgatatc cggagatgcg acgtttcgcg ggtagccacc gctttcaccc gtgtatgaag
    13921 ggtcaaaagc cagagtgaca aacccccgtt cagccagcgt ctgcgcatac aggccgctgg
    13981 attgctctta caccgccccg aaggggccac taacagcaat tgctgccagc ttgcgatcgc
    14041 cacgatcctt cggtatatag agatccccga ctaaggtgat cccgtaccgg ttcggaaagg
    14101 tcaccttacg gtgatcaact ttcgtgctct cagcaaaagt tttatcccat ttctcgacct
    14161 gcgaaacggg cgcgttcgga ttggttgtat cagcgtaact catatttgtg actccactta
    14221 atgatacgca taacagcatg gtgggtatcg cggctgtcaa tttttgggtg aaagcagtca
    14281 tcaaaatccc cctcataaaa tatgccagat actggtttca tacggataac actgctgtgc
    14341 tttgttgcaa cgcgcacatt atagttagca ttaatagtgc taattagagg gtaaaaaatg
    14401 ctaagattaa taacatattg ttctgaattg tgatgcagca ggggaaggat gatggcgaaa
    14461 cgggaaaact ataacgatct ctatctgttt atgcaggtcg tgcgagaagg gagttttacc
    14521 gccgccgcgc agaggcttgg cctggcgcaa tcaggaatta gccgtgcagt gcgcgagctg
    14581 gaagagcgac tgggcgtgca gcttctggta cgcaccacgc gcaagttatc gctcacccat
    14641 gccggcgagt tactctatcg aaacgtcgaa tctggatttg acgccttaga tatggggctt
    14701 gccacactgg cccattatcg ccatacacca tcgggtacgg tccgaattaa tgcgagccag
    14761 cacgccattg ataaagtgct gctgccgaag ctggcggtgt ttaaacaacg atacccggat
    14821 attcggctgg agctcatcag cgaaagccgg tttgtcgata ttattactga gcgcttcgat
    14881 gccggtatac gtctgggctc ggaagtggat agcggtatgg ttgcggtgcg catttcgccc
    14941 gatatggaga tggctgttgt cggcacgccc gggcactttc agcgctacgg atttccacaa
    15001 acccccaccg atttagtagg tcatccctgc atcgcttacc agttcggcga cggcagtctg
    15061 tatgcctggg aactcaatca ggacggtaaa aagatcaccc atcagcctca gggacaatgg
    15121 gcctttgctg acagttatat ggaagccaaa gccgcaaggc taggtctggg gctggcttat
    15181 gttccggagg aattagtgtc tgatgatata gaacaggggg cgctaatcag agttttgcaa
    15241 tgctacagcc aacgcctgga gggttcatat atctattatc cgcaccgcaa tgtgtcaccc
    15301 gccctacgag cggttatcga tacattgaga atttaagggg tgaagatgag atggttcgca
    15361 ggcttaggta atagttggaa aacaatgtcc gctcctggca cagagcggac tgtcagatta
    15421 gattcagacc tgtactcatt agctgataca aatcagccac atatacctgc attccaaata
    15481 atttcaggca gatattatct ccgtgaatcc ccccgccatc tttccggtgt gcggggtttt
    15541 gtcttttttc accgggaata catgtatgaa tccgtctgat gccattgagg caattgaaaa
    15601 accgctctcc tccctgcctt actcactttc ccgtcacatc ctggaacatc tgcgcaaact
    15661 caccagtcac gaacccgtga ttggcattat gggtaaaagc ggggccggta aatcctcact
    15721 ctgtaatgcg ctgtttcagg gggaggtcac accggtcagt gatgttcacg ccggcacccg
    15781 agaagtgcag cgcttccgtc tgagtggcca cggtcacagc atgattatca ctgacctgcc
    15841 cggggtgggc gagagccggg acagggatgc agagtatgaa gccctgtacc gtgacattct
    15901 gcctgaactg gacctggtac tgtggctgat taaagccgat gaccgtgccc tgtctgtgga
    15961 tgagtatttc tggcgacaca tcctgcagtg cggacatcag caggtgctgt ttgtggtgac
    16021 gcaggccgat aaaacggagc cctgccatga atgggatatg gccggtattc agccttcccc
    16081 cgcacaggta cagaacattc gcgaaaaaac ggaggcggta ttccggctgt tccggcccgt
    16141 acatccggtt gtggccgtat cggcccgcac cggctgggaa ctggatacac tggtcagtgc
    16201 gctcatgaca gcacttcccg accatgccgc cagccccctg atgacccgcc tgcaggacga
    16261 gctgcgcacg gaatctgtcc gggggcaggc ccgtgaacag tttaccggtg cggtggaccg
    16321 gatatttgac acggtggaga gcgtctgtgt tgcctctgtt gcccgtacgg ttctgcgtgc
    16381 cgtccgtgac acggtggtct ctgttgcccg cgcggtatgg aactggattt tcttctgaac
    16441 actctgccag gggaaatgcc tgattacatt gcgttaacca actgaaaaca tgacacaaat
    16501 acctcacccc ctcctgtttc tcttgcgtga ccgccctgct atcaatagaa taaaacgatc
    16561 gataaaacag gtatcgatcg ttactatcga tcgtttatag tggttaatgg tctgcgaaaa
    16621 ggcagctgaa tctcttcatt atgcagaacg gaattgcaca caacggactg atacttctca
    16681 ggcggatgag agaggggaag catttatgtc cgggaaaaac cactttatct gaccatgctg
    16741 ttttgtacct gccggtatcc acatttgtgg gtaccggctt ttttattcac cctctgtaag
    16801 gaaaagctga tgaaacgaca tctgaacacc agctacaggc tggtatggaa tcacattacg
    16861 ggcaccctgg tggtggcctc cgaactggcc cgctcacggg gaaaacgcgc cggtgtggcg
    16921 gttgcgctgt ctcttgctgc tgtcacatca ctcccggtac tggctgctga cacggttgta
    16981 caggcgggag aaaccgtgag cggcggagca ctggaaaatc atgacaacca gattgtcttc
    17041 ggtacagcca acggaatgac catcagtacc ggtctggagt atgggccaga taacgaggcc
    17101 aataccggcg ggcaatggat acaaaatggc ggtatcgcca acaacactac tgtcaccggt
    17161 ggtggtcttc agagagtgaa tgccggagga agcgtttcag acacggttat cagggccgga
    17221 ggcggacaga gccttcaggg gcaggcagtg aacaccactc tgaacggcgg tgagcagtgg
    17281 gtacatgaag gcgggattgc aacgggtacc gtcattaatg agaagggctg gcaggccgtc
    17341 aaatccggcg caatggcaac cgacacggtt gtgaatactg gcgcggaagg aggaccggat
    17401 gcggaaaatg gtgataccgg gcagaccgtc tacggagatg ccgtacgcac caccatcaat
    17461 aaaaatggtc gtcagattgt ggctgctgaa ggaacggcaa ataccactgt ggtttatgcc
    17521 ggcggcgacc agactgtaca tggtcacgca ctggatacca cgctgaatgg ggggtaccag
    17581 tatgtgcaca acggaggtac agcatctgac actgttgtta acagtgacgg ctggcagatt
    17641 atcaaggaag gtggtctggc ggatttcacc accgttaacc agaaaggtaa actgcaggtg
    17701 aacgccggtg gtacagccac gaatgtcacc ctgaagcagg gcggcgcact ggtcaccagt
    17761 acggcggcaa ccgtcaccgg cagcaaccgt ctgggcaatt tcacggtgga aaacggtaag
    17821 gctgacggcg ttgttctgga gtccggcggt cgcctggatg tactggaggg ccattcagcg
    17881 cagaaaacac gggtggatga cggcggtacc ctggcagtgt ctgccggcgg taaggcaacc
    17941 ggtgtcacca tgacatccgg tggtgccctg attgcagaca gtggtgccac tgttgagggg
    18001 accaatgcca gcggtaggtt cagtattgat ggcatatctg gtcaggccag cggcctgctg
    18061 ctggaaaatg gcggcagctt tacggttaat gccgggggac aggcaggcaa caccactgtc
    18121 ggacatcgcg gaacactgac gctggctgcc gggggaagtc tgagtggcag aacacagctc
    18181 agtaaaggcg ccagcatggt actgaatggc gatgtggtca gtaccggcga tattgttaac
    18241 gcaggggaga ttcactttga tactcagatg acacaggatg ccgtactgag ccgtgctgtt
    18301 gcaaaaggcg cctccccggt aacgttccat aaactgacca ccagtaacct cactggtcag
    18361 ggtggcacca tcaatatgcg tgttcgcctt gatggcagca atgcctctga ccagctggtg
    18421 attaatggtg gtcaggcaac cggcaaaacc tggcttgcgt ttacaaatgt cggaaacagt
    18481 aacctcgggg tggcaacctc cggacagggt atccgggttg tggatgcaca gaatggtgcc
    18541 accacagaag aaggtgcgtt tgccctgagt cgcccgcttc aggccggcgc ctttaactac
    18601 accctgaacc gtgacagcga tgaagactgg tacctgcgca gtgaaaatgc ttatcgtgca
    18661 gaagtccccc tgtatgcctc catgctgaca caggcaatgg actatgaccg gattctggca
    18721 ggctcccgca gccatcagac cggtgtaaac ggtgaaaata acagcatccg tctcagcatt
    18781 cagggcggtc atctcggtca cgataacaac ggcggtattg cccgtggggc cacgccggaa
    18841 agcagcggca gctatggcct cgtccgtctg gagggtgacc tgttgagaac agaggttgcc
    18901 ggtatgtcac tgacgacagg agtgtatggt gctgcaggcc attcttccgt tgatgttaag
    18961 gatgatgacg gctcccgtgc cggcacggtc cgggatgatg ccggcagtct gggcggatac
    19021 ctgaatctgg tacacacgtc ctccggcctg tgggctgaca ttgtggcaca gggaacccgc
    19081 cacagcatga aagcgtcatc ggacaataac gacttccgcg cccggggctg gggctggctg
    19141 ggctcactgg aaaccggtct gcccttcagt atcactgaca atctgatgct ggagccacaa
    19201 ctgcagtaca cctggcaggg actctccctg gatgacggcc aggataacgc cggttatgtg
    19261 aagttcgggc atggcagtgc acaacatgtg cgtgccggct tccgtctggg cagccacaac
    19321 gatatgaact ttggtaaagg cacctcatcc cgtgacaccc tgcgcgacag tgccaaacac
    19381 agtgtgcgtg aactgcaggt gaactggtgg gtacagcctt ctgttatccg caccttcagc
    19441 tcccggggag acatgagcat gggtacagcc gcagccggca gtaacatgac gttctcaccg
    19501 tcccagaatg gcacgtcact ggacctgcag gccggactgg aagcccgtgt ccgggaaaat
    19561 atcaccctgg gcgttcaggc cggttatgcc cacagcgtca gcggcagcag cgctgaaggt
    19621 tataacggtc aggccacact gaacgtgact ttctgataat caggtcactc cccttcaggc
    19681 cccggtcatt atgaccgggg ccttttactt tcagggagta acagatatgc cgggttgtac
    19741 atcacgttta ttgcccgaag gcccttttgc tgccaaatgg catctgaatc gatcacagtc
    19801 tggccggtta ttaccggatc ccgatggggt tattgggtga tctccttcca taaaaatacg
    19861 cagctcgcca gtgaagggct tctcagacaa taaacgtcat tcatttcatt tgtcaggccg
    19921 cgtcttctcc gggagacgca gctttttcat ttataccgct aactcattca taaggagcaa
    19981 atcatgcgat tagcaagtcg ttttggtcat gtaaaccaga tacgccgtga ccgtccgctg
    20041 acacatgaag aactgatgca ctatgtaccc agtatttttg gggaagaccg gcatacctcc
    20101 cgcagtgaac ggtatgcgta cattcccacc atcaccgtac tggaaagcct gcagcgggaa
    20161 ggttttcagc cattcttcgc ctgccagacc cgcgtgcgcg accagagccg ccggggatac
    20221 accaaacaca tgctgcgtct gcgccgtgac ggggagagaa acggacaaca tgtccctgaa
    20281 atcatcctac tgaactccca tgacggtacc tccagctacc agatgctgcc gggttacttc
    20341 aggttcgtct gccagaacgg ctgcgtctgt ggtctgtctc tgggggaagt gcgtgttcca
    20401 caccggggag acgtggtgga gaaagttatt gaaggggctt acgaggtggt gggcgttttt
    20461 gaccggattg aggaaaagcg tgatgccatg cagtctctgg ttctgccgcc accggcacgt
    20521 caggcgctgg cacaggcggc actgacttac cgttatggtg atgaacatca gcccgtcacc
    20581 actgccgaca ttctgacacc acgacgccgg ga
//
LOCUS       DAMZFT010000085        20195 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.085, whole genome shotgun sequence.
ACCESSION   DAMZFT010000085 DAMZFT010000000
VERSION     DAMZFT010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20195)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20195)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20195
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.085"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..341
                     /locus_tag="PN977_003246"
     CDS             <1..341
                     /locus_tag="PN977_003246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDI6043252.1"
                     /translation="AKSGLNRSVLDQGWYEMRRQLEYKQLWRGGQVLAVPPAYTSQRC
                     TCCGHTAKENRLSQSQFRCQVCGYTANADVNGARNILAAGHAVLACGGMVQSGRPLKQ
                     EPTEMIQATA"
     gene            complement(386..514)
                     /locus_tag="PN977_003247"
                     /pseudo
     CDS             complement(386..514)
                     /locus_tag="PN977_003247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414946.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
     gene            665..1114
                     /gene="nrdR"
                     /locus_tag="PN977_003248"
     CDS             665..1114
                     /gene="nrdR"
                     /locus_tag="PN977_003248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HDI6043253.1"
                     /translation="MHCPFCFAVDTKVIDSRLVGEGSSVRRRRQCLVCNERFTTFEVA
                     ELVMPRVVKSNDVREPFNEEKLRSGMLRALEKRPVSSDDVEMAINHIKSQLRATGERE
                     VPSKMIGNLVMEQLKKLDKVAYIRFASVYRSFEDIKEFGEEIARLED"
     gene            1118..2221
                     /gene="ribD"
                     /locus_tag="PN977_003249"
     CDS             1118..2221
                     /gene="ribD"
                     /locus_tag="PN977_003249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HDI6043254.1"
                     /translation="MQDEYYMARALKLAQRGRFTTHPNPNVGCVIVKDGETVGEGYHQ
                     RAGEPHAEVHALRMAGEKAKGATAYVTLEPCSHHGRTPPCCDALIAAGVARVVAAMQD
                     PNPQVAGRGLYRLQQAGIDVSHGLMMSEAEQLNKGFLKRMRTGFPYIQLKLGASLDGR
                     TAMASGESQWITSPQARRDVQRLRAQSHAILTSSATVLADDPALTVRWSELDEQTQAL
                     YPQQNLRQPIRIVIDSQNRVTPEHRIVQQPGETWFARTQDDSREWPETVRTLLIPEHK
                     GHLDLVVLMMQLGKQQINSIWVEAGPTLAGALLQAGLVDELIVYIAPKLLGSDARGLC
                     SLPGLEKLADAPQFKFKEIRHVGPDVCLHLVGA"
     gene            2310..2780
                     /gene="ribE"
                     /locus_tag="PN977_003250"
     CDS             2310..2780
                     /gene="ribE"
                     /locus_tag="PN977_003250"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HDI6043255.1"
                     /translation="MNIIEANVATPDARVAITIARFNNFINDSLLEGAIDALKRIGQV
                     KDENITVVWVPGAYELPLAAGALAKTGKYDAVIALGTVIRGGTAHFEYVAGGASNGLA
                     HVAQDSEIPVAFGVLTTESIEQAIERAGTKAGNKGAEAALTALEMINVLKAIKA"
     gene            2800..3219
                     /gene="nusB"
                     /locus_tag="PN977_003251"
     CDS             2800..3219
                     /gene="nusB"
                     /locus_tag="PN977_003251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HDI6043256.1"
                     /translation="MKPAARRRARECAVQALYSWQLSQNDIADVEYQFLAEQDVKDVD
                     VLYFRELLAGVATNTAYLDGLMKPYLSRLLEELGQVEKAVLRIALYELSKRSDVPYKV
                     AINEAIELAKSFGAEDSHKFVNGVLDKAAPVIRPNKK"
     gene            3297..4274
                     /gene="thiL"
                     /locus_tag="PN977_003252"
     CDS             3297..4274
                     /gene="thiL"
                     /locus_tag="PN977_003252"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HDI6043257.1"
                     /translation="MACGEFSLIARYFDRVRSSRLDVELGIGDDCALLNIPEKQTLAI
                     STDTLVAGNHFLPDIDPADLAYKALAVNLSDLAAMGADPAWLTLALTLPDVDEAWLES
                     FSDSLFDLLNYYDMQLIGGDTTRGPLSMTLGIHGFVPMGRALTRAGAKPGDWIYVTGT
                     PGDSAAGLAILQNRLQVADAKDADYLIKRHLRPSPRILQGQALRDLANSAIDLSDGLI
                     SDLGHIVKASDCGARIDLALLPFSDALSRHVEPEQALRWALSGGEDYELCFTVPELNR
                     GALDVALGHLGVPFTCIGQMTADIEGLCFIRDGEPVTLDWKGYDHFATP"
     gene            4252..4770
                     /gene="pgpA"
                     /locus_tag="PN977_003253"
     CDS             4252..4770
                     /gene="pgpA"
                     /locus_tag="PN977_003253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HDI6043258.1"
                     /translation="MTILPRHKDVAKSRLKMSNPWHLLAVGFGSGLSPIVPGTMGSLA
                     AIPFWYLMTFLPWQLYSLVVMLGICIGVYLCHQTAKDMGVHDHGSIVWDEFIGMWITL
                     MALPTNDWQWVAAGFVIFRILDMWKPWPIRWFDRNVHGGMGIMIDDIVAGVISAGILY
                     FIGHHWPLGILS"
     gene            complement(4824..5798)
                     /gene="yajO"
                     /locus_tag="PN977_003254"
     CDS             complement(4824..5798)
                     /gene="yajO"
                     /locus_tag="PN977_003254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxyxylulose-5-phosphate synthase YajO"
                     /protein_id="HDI6043259.1"
                     /translation="MQYNPLGKTDLRVSRLCLGCMTFGEPDRGNHAWTLPEESSRPII
                     KRALEGGINFFDTANSYSDGSSEEIVGRALRDFARREDVVVATKVFHRVGDLPEGLSR
                     AQILRSIDDSLRRLGMDYVDILQIHRWDYNTPIEETLEALNDVVKAGKARYIGASSMH
                     ASQFAQALELQKQHGWAQFVSMQDHYNLIYREEEREMLPLCYQEGVAVIPWSPLARGR
                     LTRPWGETTARLVSDEVGKNLYQESDENDAQIAERLTGVSEELGATRAQVALAWLLSK
                     PGIAAPIIGTSREEQLDELLNAVDITLKPEQIAELETPYKPHPVVGFK"
     gene            complement(5853..7715)
                     /gene="dxs"
                     /locus_tag="PN977_003255"
     CDS             complement(5853..7715)
                     /gene="dxs"
                     /locus_tag="PN977_003255"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HDI6043260.1"
                     /translation="MSFDIAKYPTLALVDSTQELRLLPKESLPKLCDELRRYLLDSVS
                     RSSGHFASGLGTVELTVALHYVYNTPFDQLIWDVGHQAYPHKILTGRRDKIGTIRQKG
                     GLHPFPWRGESEYDVLSVGHSSTSISAGIGIAVAAEKEGKNRRTVCVIGDGAITAGMA
                     FEAMNHAGDIRPDMLVVLNDNEMSISENVGALNNHLAQLLSGKLYSSLREGGKKVFSG
                     VPPIKELLKRTEEHIKGMVVPGTLFEELGFNYIGPVDGHDVLGLITTLKNMRDLKGPQ
                     FLHIMTKKGRGYEPAEKDPITFHAVPKFDPSSGCLPKSSGGLPSYSKIFGDWLCETAA
                     KDNKLMAITPAMREGSGMVEFSRKFPDRYFDVAIAEQHAVTFAAGLAIGGYKPIVAIY
                     STFLQRAYDQVLHDVAIQKLPVLFAIDRAGIVGADGQTHQGAFDLSYLRCIPEMVIMT
                     PSDENECRQMLYTGYHYNDGPSAVRYPRGNAVGVELTPLEKLPIGKGIVKRRGEKLAI
                     LNFGTLMPEAAKVAESLNATLVDMRFVKPLDEALILEIAASHEALVTVEENAIMGGAG
                     SGVNEVLMAHRKPVPVLNIGLPDFFIPQGTQEEMRAELGLDAAGMEAKIKAWLA"
     gene            complement(7740..8639)
                     /gene="ispA"
                     /locus_tag="PN977_003256"
     CDS             complement(7740..8639)
                     /gene="ispA"
                     /locus_tag="PN977_003256"
                     /EC_number="2.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl diphosphate synthase"
                     /protein_id="HDI6043261.1"
                     /translation="MDFPQQLEACVKQANQALSRFIAPLPFQNTPVVETMQYGALLGG
                     KRLRPFLVYATGHMFGVSTNTLDAPAAAVECIHAYSLIHDDLPAMDDDDLRRGLPTCH
                     VKFGEANAILAGDALQTLAFSILSDADMPEVSDRDRISMISELASASGIAGMCGGQAL
                     DLDAEGKHVPLDALERIHRHKTGALIRAAVRLGALSAGDKGRRALPVLDKYAESIGLA
                     FQVQDDILDVVGDTATLGKRQGADQQLGKSTYPALLGLEQARKKARDLIDDARQSLKQ
                     LAEQSLDTSALEALADYIIQRNK"
     gene            complement(8639..8881)
                     /gene="xseB"
                     /locus_tag="PN977_003257"
     CDS             complement(8639..8881)
                     /gene="xseB"
                     /locus_tag="PN977_003257"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HDI6043262.1"
                     /translation="MPKKNEAPASFEKALSELEQIVTRLESGDLPLEEALNEFERGVQ
                     LARQGQAKLQQAEQRVQILLSDNEDASLTPFTPDNE"
     gene            9087..10535
                     /gene="thiI"
                     /locus_tag="PN977_003258"
     CDS             9087..10535
                     /gene="thiI"
                     /locus_tag="PN977_003258"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HDI6043263.1"
                     /translation="MKFIIKLFPEITIKSQSVRLRFIKILTGNIRNVLKHYDETLAVV
                     RHWDNIEVRAKDENQRLAIRDALTRIPGIHHILEVEDVPFTDMHDIFEKALVQYRDQL
                     EGKTFCVRVKRRGKHDFSSIDVERYVGGGLNQHIESARVKLTNPDVTVHLEVEDDRLL
                     LIKGRYEGIGGFPIGTQEDVLSLISGGFDSGVSSYMLMRRGCRVHYCFFNLGGAAHEI
                     GVRQVAHYLWNRFGSSHRVRFVAINFEPVVGEILEKIDDGQMGVILKRMMVRAASKVA
                     ERYGVQALVTGEALGQVSSQTLTNLRLIDNVSDTLILRPLISYDKEHIINLARQIGTE
                     DFARTMPEYCGVISKSPTVKAVKSKIEAEEEKFDFSILDKVVEEANNVDIREIAQQTE
                     QEVVEVETVNGFGPNDVILDIRSIDEQEDKPLKVEGIDVVSLPFYKLSTKFGDLDQSK
                     TWLLWCERGVMSRLQALYLREQGFNNVKVYRP"
     gene            complement(10589..11179)
                     /gene="yajL"
                     /locus_tag="PN977_003259"
     CDS             complement(10589..11179)
                     /gene="yajL"
                     /locus_tag="PN977_003259"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein deglycase YajL"
                     /protein_id="HDI6043264.1"
                     /translation="MSASALVCLAPGSEETEAVTTIDLLVRGGIKVTTASVASDGNLA
                     ITCSRGVKLLADAPLVEVADGEYDVIVLPGGIKGAECFRDSTLLVETVKQFHRSGRIV
                     AAICAAPATVLVPHDIFPIGNMTGFPTLKDKIPAEQWQDKRVVWDARVKLLTSQGPGT
                     AIDFGLKIIDLLVGREKAHEVASQLVMAAGIYNYYE"
     gene            complement(11142..12053)
                     /gene="panE"
                     /locus_tag="PN977_003260"
     CDS             complement(11142..12053)
                     /gene="panE"
                     /locus_tag="PN977_003260"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HDI6043265.1"
                     /translation="MKITVLGCGALGQLWLTALCKQGHEVQGWLRVPQPYCSVNLVET
                     DGSIFNESLTANDPDFLATSDLLLVTLKAWQVSDAVKSLASTLPVTTPILLIHNGMGT
                     IEELQNIQQPLLMGTTTHAARRDGNVIIHVANGITHIGPARQQDGDYSYLADILQTVL
                     PDVAWHNNIRAELWRKLAVNCVINPLTATWNCPNGELRHHPQEIMQICEEVAAVIERE
                     GHHTSAEDLRDYVMQVIDATAENISSMLQDIRALRHTEIDYINGFLLRRARAHGIAVP
                     ENTRLFEMVKRKESEYERIGTGLPRPW"
     gene            12221..12712
                     /gene="yajQ"
                     /locus_tag="PN977_003261"
     CDS             12221..12712
                     /gene="yajQ"
                     /locus_tag="PN977_003261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414960.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide binding protein YajQ"
                     /protein_id="HDI6043266.1"
                     /translation="MPSFDIVSEVDLQEARNAVDNASREVESRFDFRNVEASFELNDA
                     SKTIKVLSESDFQVNQLLDILRAKLLKRGIEGSSLDVPENIVHSGKTWFVEAKLKQGI
                     ESATQKKIVKMIKDSKLKVQAQIQGDEIRVTGKSRDDLQAVMAMVRGGDLGQPFQFKN
                     FRD"
     gene            complement(12840..14204)
                     /locus_tag="PN977_003262"
     CDS             complement(12840..14204)
                     /locus_tag="PN977_003262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDI6043267.1"
                     /translation="MNDYKMTPGERRATWGLGTVFSLRMLGMFMVLPVLTTYGMALQG
                     ASEALIGIAIGIYGLTQAVFQIPFGLLSDRIGRKPLIVGGLAVFAAGSVIAALSDSIW
                     GIILGRALQGSGAIAAAVMALLSDLTREQNRTKAMAFIGVSFGITFAIAMVLGPIITH
                     KLGLHALFWMIAILATTGIALTIWVVPNSSTHVLNRESGMVKGSFSKVLAEPRLLKLN
                     FGIMCLHMLLMSTFVALPGQLADAGFPAAEHWKVYLATMLIAFGSVVPFIIYAEVKRK
                     MKQVFVFCVGLIVVAEIVLWNAQTQFWQLVVGVQLFFVAFNLMEALLPSLISKESPAG
                     YKGTAMGVYSTSQFLGVAIGGSLGGWIDGMFDGQGVFLAGAMLAAVWLAVASTMKEPP
                     YVSSLRIEIPADIAANEALKVRLLETEGVKEVLIAEEEHSAYVKIDSKVTNRFEVEQA
                     IRQA"
     gene            14611..15546
                     /locus_tag="PN977_003263"
     CDS             14611..15546
                     /locus_tag="PN977_003263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001427098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HDI6043268.1"
                     /translation="MLTKRILFSLMLIVSPCVVASEKAHELYDSIYGGQPAPDVINRL
                     HKMAELGDVDAQSLLGWEYYQPRFDTKPDVQEAIKWFELAAKHGDREAPLALGSIYYD
                     GELVRVDYAKAYALFNQAAQYGVNLAWSRLGMMYANGQYVEVDCKKAKEYLDKGVHIY
                     GGPEDFLATCRKDMIDRKTVDDTLPVITVTRSGMRDNFLDKGFSCMDSLFATTNKLGE
                     VANLRVTFSIRSPSGKEINQTVGFAPFGLNRLNISFTDYIFDSFTSNSSLILYKPEFE
                     RKSCATVRTTIVAATATINGKDVELLKAGAIEQKW"
     gene            complement(15602..16144)
                     /locus_tag="PN977_003264"
     CDS             complement(15602..16144)
                     /locus_tag="PN977_003264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000227782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDI6043269.1"
                     /translation="MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRAS
                     LKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEKQRVSGVLQGSQPSEIGVVRLVM
                     LGVARKYQKRGFGQDLLCDFFEHVKIIHRALPIKGVYLDADPAAINFYTRLGFVQLSA
                     RPNVFGAVPMFLAIQHILAA"
     gene            complement(16128..16439)
                     /locus_tag="PN977_003265"
     CDS             complement(16128..16439)
                     /locus_tag="PN977_003265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706319.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HDI6043270.1"
                     /translation="MPGKTVALPDIDKTLKNARVELKTSPDAKNKLREAAQAVGVDLS
                     AFILSAAMERAESVLDNQRRRELSNQSWELMNQLIAEPAQPTLALKALMKRKNSDGRQ
                     A"
     gene            complement(16624..17514)
                     /gene="cyoE"
                     /locus_tag="PN977_003266"
     CDS             complement(16624..17514)
                     /gene="cyoE"
                     /locus_tag="PN977_003266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX farnesyltransferase"
                     /protein_id="HDI6043271.1"
                     /translation="MMFKQYLQVTKPGIIFGNLISVIGGFLLASKGSIDYPLFIYTLV
                     GVSLVVASGCVFNNYIDRDIDRKMERTKNRVLVKGLISPAVSLVYATLLGIAGFMLLW
                     FGANPLACWLGVMGFVVYVGVYSLYMKRHSVYGTLIGSLSGAAPPVIGYCAVTGEFDS
                     GAAILLAIFSLWQMPHSYAIAIFRFKDYQAANIPVLPVVKGISVAKNHITLYIIAFAV
                     ATLMLSLGGYAGYKYLVVAAAVSVWWLGMALRGYKVADDRIWARKLFGFSIIAITALS
                     VMMSVDFMVPDSHTLLAAVW"
     gene            complement(17526..17855)
                     /locus_tag="PN977_003267"
     CDS             complement(17526..17855)
                     /locus_tag="PN977_003267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit IV"
                     /protein_id="HDI6043272.1"
                     /translation="MSHSTDHSGASHGSVKTYMTGFILSIILTVIPFWMVMTGAASPA
                     VILGTILAMAVVQVLVHLVCFLHMNTKSDEGWNMTAFVFTVLIIAILVVGSIWIMWNL
                     NYNMMMH"
     gene            complement(17855..18469)
                     /locus_tag="PN977_003268"
     CDS             complement(17855..18469)
                     /locus_tag="PN977_003268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit III"
                     /protein_id="HDI6043273.1"
                     /translation="MATDTLTHATAHAHEHGHHDAGGTKIFGFWIYLMSDCILFSILF
                     ATYAVLVNGTAGGPTGKDIFELPFVLVETFLLLFSSITYGMAAIAMYKNNKSQVISWL
                     ALTWLFGAGFIGMEIYEFHHLIVNGMGPDRSGFLSAFFALVGTHGLHVTSGLIWMAVL
                     MVQIARRGLTSTNRTRIMCLSLFWHFLDVVWICVFTVVYLMGAM"
     gene            complement(18459..>20195)
                     /gene="cyoB"
                     /locus_tag="PN977_003269"
     CDS             complement(18459..>20195)
                     /gene="cyoB"
                     /locus_tag="PN977_003269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002444673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit I"
                     /protein_id="HDI6043274.1"
                     /translation="ASAGEAGFLPPHHYDQIFTAHGVIMIFFVAMPFVIGLMNLVVPL
                     QIGARDVAFPFLNNLSFWFTVVGVILVNVSLGVGEFAQTGWLAYPPLSGIEYSPGVGV
                     DYWIWSLQLSGIGTTLTGINFFVTILKMRAPGMTMFKMPVFTWASLCANVLIIASFPI
                     LTVTVALLTLDRYLGTHFFTNDMGGNMMMYINLIWAWGHPEVYILILPVFGVFSEIAA
                     TFSRKRLFGYTSLVWATVCITVLSFIVWLHHFFTMGAGANVNAFFGITTMIIAIPTGV
                     KIFNWLFTMYQGRIVFHSAMLWTIGFIVTFSVGGMTGVLLAVPGADFVLHNSLFLIAH
                     FHNVIIGGVVFGCFAGMTYWWPKAFGFKLNETWGKRAFWFWIIGFFVAFMPLYALGFM
                     GMTRRLSQQIDPQFHTMLMIAASGAVLIALGILCLVIQMYVSIRDRDQNRDLTGDPWG
                     GRTLEWATSSPPPFYNFAVVPHVHERDAFWEMKEKGEAYKKPDHYEEIHMPKNSGAGI
                     VIAAFSTIFGFAMIWHIWWLAIVGFAGMIITWIVKSFDEDVDYYVPVAEIEKLENQHF
                     DEITKAGLKNGN"
BASE COUNT         4958 a         5155 c         5312 g         4770 t
ORIGIN      
        1 gggcaaaatc aggtttaaac cgttcggtac tggatcaggg ctggtatgaa atgcgccgcc
       61 agcttgagta caagcagctc tggcgtggcg gtcaggtgct tgctgttccg ccagcgtaca
      121 caagccagcg ttgcacgtgc tgtggtcata cagcgaaaga aaatcgcctg tcacaaagtc
      181 aattcagatg ccaggtatgt ggatatacag cgaacgccga tgtaaatggc gctcgtaaca
      241 ttttagcggc ggggcacgcc gttcttgcct gtggagggat ggtgcagtca ggccgcccgt
      301 tgaagcagga acccaccgaa atgattcagg cgacagcctg aacgtagcag ggatccacgt
      361 ccttcagggc gtggaggatg tcaatgagtt gcgtcatctc ctggttcagt gtgctgacgc
      421 tgtgtttcat ctgccgcact tcgctttgct gcacacaggc gctaaggctg aacaggcttc
      481 ccagcaggag cagtcgaaaa acgttagtat tcataggtcg tccctttctc gtcgcaatta
      541 gcctaatggt agagggtaaa agcagtgaaa gcatcgatcc tttatctcaa aagcgttgcg
      601 cctttgttgt atcgtcagtt cagggtaaaa tagatttctg ttaaccacct ggtcaggacg
      661 ccgtatgcat tgcccattct gtttcgccgt ggacactaag gtaattgact ctcgtctcgt
      721 gggcgagggt tcatccgtac gccgccgtcg gcagtgtctg gtgtgtaatg aacgtttcac
      781 cacctttgaa gtggcggagc tggttatgcc gcgtgttgta aaaagcaacg acgtgcgtga
      841 accgtttaat gaagagaaat tacgtagcgg aatgctgcgg gcgctggaaa aacgtccggt
      901 gagttccgat gacgtcgaaa tggcaatcaa tcatataaaa tcgcagctgc gcgccaccgg
      961 tgagcgcgaa gtgccgagca agatgattgg caatctggtg atggagcaat tgaaaaagct
     1021 cgataaagtc gcctatatcc gttttgcctc tgtctaccgc agtttcgaag atatcaaaga
     1081 atttggcgaa gagatcgcgc gcctggagga ctaagccgtg caggacgagt attacatggc
     1141 gcgggcgcta aagctggcgc aacgaggacg ttttaccacg catcccaacc cgaatgtcgg
     1201 gtgcgtcatt gtcaaagatg gcgaaactgt cggtgaaggt taccatcaac gagcgggtga
     1261 accgcatgcc gaggtacacg cgttgcgcat ggccggcgaa aaagccaaag gcgcaacggc
     1321 gtatgtcaca ctcgaaccct gtagccatca tggtcgtacg ccaccgtgtt gtgatgcatt
     1381 aattgctgcg ggcgtggcgc gcgtggttgc tgcaatgcaa gaccccaatc cgcaggtcgc
     1441 tgggcgtgga ctttaccgtc tacaacaggc tggtattgac gtcagccacg ggttgatgat
     1501 gagtgaagcc gagcaattga ataaaggctt tctcaagcgg atgcgcaccg gctttcctta
     1561 tattcagtta aaacttggcg catcgcttga tggccgcacg gcgatggcga gcggcgaaag
     1621 ccagtggatc acttcgcctc aggcgcggcg cgacgtacaa cgactgcgcg cacaaagtca
     1681 tgccatttta accagcagcg ccacggtgct ggcggatgat ccagccttaa cagtgcgttg
     1741 gtctgaactg gatgaacaaa ctcaggcgct ctatccgcaa caaaatctcc gtcagccgat
     1801 acgtattgtg attgatagcc aaaatcgcgt gacgccggaa catcgcattg tgcagcagcc
     1861 cggcgaaact tggttcgcgc gtactcagga tgattctcgt gagtggccgg aaacggtgcg
     1921 taccttgctg attccagagc ataaaggtca tctggatctg gttgtactga tgatgcaact
     1981 gggtaaacag caaattaaca gcatctgggt ggaagcgggg ccaacgctcg ctggcgcatt
     2041 gctacaggcg gggttagtcg atgagctgat tgtctatatc gcacctaaac tattaggcag
     2101 cgacgcccgt ggattatgct cgctgccagg gcttgagaaa ttagccgacg ccccccaatt
     2161 taaattcaaa gagatacgtc atgtaggccc ggatgtttgc ctgcatttag tgggtgcatg
     2221 atctcccggc tcgaaaggga agcagagcac gaaatattat gctaaaatcc gcccccctgc
     2281 ggggccatac tcgaacccga aggaagaaaa tgaacattat tgaagctaac gttgctaccc
     2341 cggacgctcg cgtcgccatc accatcgcgc gtttcaacaa ctttatcaat gacagcctgc
     2401 tggaaggtgc aattgacgca ctgaaacgta tcggtcaggt aaaagatgaa aacattaccg
     2461 ttgtttgggt gcctggtgcc tatgagctgc cgctggcggc gggtgcactg gctaaaaccg
     2521 gtaaatacga cgcggtgatt gcgctgggta cggttattcg tggtggcact gcccactttg
     2581 aatatgtcgc tggtggtgca agcaacggcc tggcgcatgt tgcccaggac agcgaaattc
     2641 cggttgcttt tggtgttctg accactgaaa gcattgaaca agcgatcgaa cgtgctggca
     2701 ccaaagctgg caacaaaggt gcagaagctg cactgactgc gcttgaaatg attaatgtat
     2761 tgaaagccat caaggcctga aattagtaag gggaaatccg tgaaacctgc tgctcgtcgc
     2821 cgcgctcgtg agtgtgccgt ccaggcgctc tactcctggc agttgtccca gaacgacatc
     2881 gctgatgttg aataccagtt cctggctgaa caggatgtaa aagacgttga cgtcctgtac
     2941 ttccgtgagc tgctggccgg ggtggcgact aacaccgcat acctcgacgg actgatgaag
     3001 ccatacctgt cccgcctgct ggaagaactg ggccaggtag aaaaagcagt actgcgcatt
     3061 gcgctgtacg agctgtctaa acgtagcgat gtgccataca aagtggccat taacgaagcg
     3121 atcgaactgg cgaaatcgtt cggcgcagaa gacagccata agttcgtcaa cggcgtactc
     3181 gataaagcag cacctgtgat tcgccctaac aaaaagtgat atccaggccg gtagattcac
     3241 ggaagaccgt tccgcgatcg ccggcctttt cttttttacc tgctgaggca taacgtatgg
     3301 catgtggcga gttctccctg attgcccgtt attttgaccg tgtaagaagt tctcgtcttg
     3361 atgtcgaact gggcatcggc gacgattgcg cacttctcaa tatccccgag aagcagaccc
     3421 tggcgatcag cactgatacg ctggtggcgg gcaaccactt cctccctgat atcgatcctg
     3481 ctgatctggc gtataaagca ctggcggtga acctaagcga tctggcagcg atgggggccg
     3541 atccagcctg gctgacgctg gcattaacct taccggacgt cgacgaagcg tggcttgagt
     3601 ccttcagcga cagtttgttt gatcttctca attattacga tatgcaactc attggcggcg
     3661 ataccacgcg tgggccatta tcaatgacgc tgggtatcca cggctttgtt ccgatgggac
     3721 gagccttaac gcgcgctggg gcgaaaccgg gtgactggat ctatgtgacc ggtacaccgg
     3781 gcgatagcgc cgccgggctg gcgattttgc aaaaccgttt gcaggttgcc gatgctaaag
     3841 atgcggacta cttgatcaaa cgtcatctcc gtccatcgcc gcgtatttta caggggcagg
     3901 cactgcgcga tctggcaaat tcagctatcg atctctctga cggtctgatt tccgatctcg
     3961 ggcatatcgt gaaagccagc gactgcggcg cacgtattga cctggcattg ctgccgtttt
     4021 ctgatgcgct ttctcgccat gttgaacctg aacaggcgtt gcgctgggcg ctctctggcg
     4081 gtgaagatta cgagttgtgt ttcactgtgc cggaactgaa ccgtggcgcg ctggatgtgg
     4141 ctctcggaca cctgggcgta ccgtttacct gtatcgggca aatgaccgcc gatatcgaag
     4201 ggctttgttt tattcgtgac ggcgaacctg tcacgttaga ctggaaagga tatgaccatt
     4261 ttgccacgcc ataaagatgt cgcgaaaagt cgcctgaaga tgagtaatcc gtggcatctg
     4321 cttgctgtcg gattcggaag tggattaagc ccaatcgttc ctggcacgat gggatcgctg
     4381 gcagcgattc cgttctggta tctgatgact tttttgccct ggcagctcta ttcgctggtg
     4441 gtgatgctgg ggatctgtat cggcgtgtat ctttgtcatc aaacggcgaa agacatgggt
     4501 gtgcacgatc acggcagcat tgtctgggac gaatttatcg gtatgtggat cacgctcatg
     4561 gcgctgccga ccaatgactg gcagtgggtt gccgccgggt ttgtgatttt ccgtattctg
     4621 gatatgtgga agccgtggcc gatccgttgg tttgatcgca atgtacatgg cggcatgggg
     4681 atcatgattg acgatattgt cgccggggtg atttccgcag gcatcctgta ctttattgga
     4741 catcactggc cgctgggtat tctgtcgtag ttgtgcaccg atgcctgatg tgacgcttgt
     4801 cacgtctcat caggcctgca tacctattta aatcctacta ccggatgcgg tttatacggc
     4861 gtttccagtt cagcaatctg ttccggcttt aaagtgatat ccaccgcgtt cagcagctca
     4921 tcaagctgtt cttcccgcga agttccgata atcggtgcgg caatgcccgg tttactcaac
     4981 aaccaggcca gcgcaacttg tgctcgtgtt gcaccaagtt cttcactgac gcccgttaag
     5041 cgttctgcaa tttgtgcgtc attttcatcg ctttcttggt agagattttt ccccacttca
     5101 tccgacacca gccgtgcggt ggtttctccc caaggacgcg tcagacggcc ccgcgccagc
     5161 gggctccatg gaatcaccgc cacgccttcc tgataacaca gcggcagcat ctcgcgctct
     5221 tcttcacgat aaatcagatt gtagtgatcc tgcatactga caaactgcgc ccagccgtgc
     5281 tgtttttgta gttccagtgc ttgagcaaac tgcgaagcgt gcatggatga cgcgccgata
     5341 taacgcgctt tcccggcttt taccacgtcg ttgagggctt ccagcgtctc ttcgatcggc
     5401 gtgttgtaat cccagcgatg aatttgcagg atatcgacat aatccatgcc gagacggcgc
     5461 aggctgtcgt cgatagagcg caaaatttgt gcacgggata atccttccgg taaatcacca
     5521 acgcgatgga acactttggt cgcaacgacc acgtcttcac gacgggcgaa atcccgcagt
     5581 gcgcgaccga cgatctcttc gctgctgcca tcggaatagc tattggcggt atcaaagaaa
     5641 tttatgccgc cttccagcgc gcgtttaatg atgggacggc tgctttcttc cggcagtgtc
     5701 catgcgtgat taccgcgatc tggctcgcca aaggtcatac agccgaggca aagtcgggaa
     5761 acgcgaaggt cggtttttcc taaggggttg tattgcatgc tgccactcct gccatgaata
     5821 atttcttaag catagcagga gtggagtagg gattatgcca gccaggcctt gattttggct
     5881 tccataccgg cggcatcgag gccgagttcg gcgcgcattt cttcctgtgt tccttgtgga
     5941 ataaagaagt caggcaggcc aatgttcagc acgggtactg gtttacgatg ggccatcagc
     6001 acttcgttca ctccgctgcc tgcgccgccc ataatggcgt tttcttctac ggtgaccagc
     6061 gcttcatggc tggcggctat ttccagaatt aacgcttcat caagcggttt cacaaaacgc
     6121 atatcgacca gcgtagcgtt cagcgattca gcgactttcg ccgcttctgg catcagcgta
     6181 ccaaagttaa ggatcgccag tttctcgcca cgacgcttca caatgccttt gccaattggc
     6241 agtttttcca gcggcgtcag ttccacgcca accgcgttgc cacgcgggta gcgtaccgct
     6301 gacgggccat cgttatagtg atagccggta tagagcatct ggcgacattc gttttcatcg
     6361 ctcggggtca taatgaccat ttccggtatg cagcgcaggt aagagagatc aaaagcgccc
     6421 tggtgggttt gaccgtcagc accaacaatg cccgcgcggt caatggcgaa cagaaccggg
     6481 agtttttgta tcgccacgtc atgcagcacc tgatcatagg cgcgttgcag gaaagtggag
     6541 tagatcgcga caatgggttt gtagccacca atcgccagac ccgcagcaaa ggtcactgcg
     6601 tgttgctcgg cgattgccac gtcgaagtag cgatccggga atttacgtga aaactcgacc
     6661 atgccagaac cttcgcgcat cgccggagta atcgccatca gcttgttgtc tttcgctgcc
     6721 gtttcgcaca accagtcgcc aaagattttt gaatagctcg gcaaaccgcc gctacttttc
     6781 ggcaaacaac cgctggaggg atcaaattta ggcacggcgt ggaaagtgat tgggtctttt
     6841 tctgccggtt cataaccacg accttttttg gtcatgatat gcaggaactg cgggcctttc
     6901 aggtcgcgca tgttcttcag cgtggtgata agccccagca catcgtgacc gtccaccggg
     6961 ccgatgtagt taaagcccag ctcttcaaac aacgtgccag gcactaccat gcctttaata
     7021 tgttcttcgg tacgtttgag cagctcttta atgggcggaa cgccagagaa aacttttttc
     7081 ccgccttcgc gcagtgaaga gtaaagctta ccggaaagca gttgcgccag atggttgttg
     7141 agcgcgccaa cattttcgga aatcgacatt tcattgtcgt tgagaaccac cagcatatca
     7201 ggacggatat cgcccgcgtg attcatcgct tcaaacgcca tgccagcggt aatcgcgcca
     7261 tcgccaatga cacagacggt acggcgattt ttgccttctt tctcggcagc aaccgcaata
     7321 ccaattccgg cactgatgga ggttgatgaa tgcccgacgc ttaatacgtc atattcgctt
     7381 tcgccgcgcc acgggaacgg gtgcaggccg cctttctgac ggatggtgcc gattttgtcg
     7441 cggcgtccgg tcaaaatttt atgcggataa gcctgatgcc ccacatccca gattaactgg
     7501 tcaaacgggg tattgtagac atagtgcagc gccacggtca gttcgaccgt gcccagcccg
     7561 gaggcgaagt gcccgctgga acggctcacg ctgtcgagta aatagcggcg cagttcgtcg
     7621 cagagtttcg gtaaactctc tttcggcaac agtcgtaact cctgggtgga gtcgaccagt
     7681 gccagggtcg ggtatttggc aatatcaaaa ctcatcaggg gcctattaat acttattgtt
     7741 tatttattac gctggatgat gtagtccgct agcgcttcca gtgccgaggt atcgagtgac
     7801 tgttcagcca gttgtttcag cgactgacgg gcatcgtcga tcagatcccg ggctttcttc
     7861 cgggcttgct caagacccag aagtgcaggg taggtacttt taccaagttg ctggtcagca
     7921 ccctggcgtt ttcccaacgt tgcagtatct cctaccacat ccaggatgtc atcctgaacc
     7981 tggaaggcaa ggccgatgct ctctgcgtac ttgtcgagta ctggcagagc acgacgtcct
     8041 ttatctccgg cgcttaatgc accaaggcga acggcggcgc gaatcaatgc gccggtttta
     8101 tgacgatgaa tacgctcaag cgcgtccaga ggtacgtgtt tgccttcagc gtctaaatct
     8161 aatgcctgac caccgcacat tccggcaata ccgctggcgc tcgccagctc agaaatcatc
     8221 gaaattctgt cgcgatctga cacttccggc atatcggcat cgcttagaat cgagaacgcc
     8281 agcgtttgta aagcgtcacc agcgagaatc gcgtttgctt cgccaaactt cacatggcag
     8341 gtcggcaaac cgcgacgcag atcgtcatca tccatcgccg gtaaatcatc atgaattaat
     8401 gagtaagcgt ggatacactc tacggcggca gcgggtgcgt ccagtgtgtt tgtgctaacg
     8461 ccgaacatat gaccagtggc ataaaccagg aaaggtcgca ggcgcttacc acctaataat
     8521 gcgccatact gcatggtttc gaccacggga gtgttctgaa agggcagtgg ggcgataaaa
     8581 cggctcagcg cctggttggc ctgcttaacg caggcttcga gttgctgcgg aaagtccatt
     8641 actcattgtc cggtgtaaaa ggggttagag aggcgtcttc attgtcagac agcagaattt
     8701 gcacgcgctg ttcggcttgt tgtaatttgg cctgcccctg acgtgccagt tgcacgccgc
     8761 gttcgaactc gttcagcgcc tcttccagcg gcaggtcgcc actttccaga cgggttacaa
     8821 tctgttccag ctcgctcagc gccttttcaa agctggcggg cgcctcattt ttcttcggca
     8881 taatgaatgt ctgactctca atatttttcg ccccgtcatg gtaacggact cagggcaaat
     8941 agcaaataac gcgcaatggt aaggtgatgt gcgcagcaaa gcgatgttag tggtatactt
     9001 ccgcgcctgg atgcagccgc aggtgtgggc tgctgtattt ttccctatac aagtcgctta
     9061 aggcttgcca acgaaccatt gccgccatga agtttatcat taaattgttc ccggaaatca
     9121 ccatcaaaag ccaatctgtg cgcttgcgct ttataaaaat ccttaccggg aacattcgta
     9181 acgttttaaa gcactatgat gagacgctcg ctgtcgtccg ccactgggat aacatcgaag
     9241 ttcgcgcaaa agatgaaaac cagcgtctgg ccattcgcga cgccttgacc cgtattccgg
     9301 gtatccacca tattctcgaa gtcgaagacg tgccgtttac cgacatgcac gatattttcg
     9361 agaaagcgtt ggttcagtat cgcgatcagt tggaaggcaa aaccttctgc gtgcgcgtga
     9421 agcgccgtgg caaacatgat tttagctcga ttgatgtgga gcgttacgtc ggcggcggtt
     9481 taaatcagca tattgaatcc gcgcgcgtga agttgaccaa tccggatgtg actgtccatc
     9541 tggaagtgga agacgatcgt cttctgctga ttaaaggccg ctacgaaggt attggcggtt
     9601 tcccgattgg tacccaggaa gatgtgctgt cgctcatttc tggtggtttc gactccggcg
     9661 tttccagtta tatgttgatg cgtcgcggat gtcgtgtgca ttactgcttc tttaaccttg
     9721 gcggcgcggc gcatgaaatt ggcgtgcgtc aggtagcgca ttatctgtgg aaccgctttg
     9781 gcagctccca ccgcgtgcgt tttgtcgcta ttaatttcga accggtcgtc ggggaaattc
     9841 tcgagaaaat cgacgacggt cagatgggcg ttatcctcaa acgtatgatg gtgcgtgccg
     9901 cgtccaaagt ggcagaacgt tacggcgttc aggcgctggt taccggtgaa gcgctcggcc
     9961 aggtgtccag ccagacgctg accaacctgc gcctgattga taacgtctcc gatacgttga
    10021 tcctgcgtcc gctgatctct tacgacaaag agcacatcat caacctggct cgccagattg
    10081 gcaccgaaga ctttgcccgc acgatgccgg aatattgcgg cgtgatttcc aaaagcccga
    10141 cggtgaaagc ggttaaatcg aagattgaag cggaagaaga gaagtttgac ttcagcattc
    10201 tcgataaagt ggttgaggaa gcgaataacg ttgatatccg cgaaatcgcc cagcagaccg
    10261 agcaggaagt ggtggaagtg gaaaccgtca atggcttcgg cccgaacgac gtgatcctcg
    10321 atatccgttc tatcgatgaa caggaagata agccactgaa agtcgaaggg attgatgtgg
    10381 tttctctgcc gttctataaa ctgagcacca aatttggcga tctcgaccag agcaaaacct
    10441 ggctgttgtg gtgtgagcgc ggggtgatga gccgcctgca ggcgctctat ctgcgcgagc
    10501 agggctttaa caatgtgaag gtgtatcgcc cgtaatatgt ggttgtaacg tcgcatctgg
    10561 tcagatgcga cgtttaccgc atccgacact actcgtaata attataaatc cctgccgcca
    10621 tcaccagttg tgatgctact tcatgggctt tttcacggcc aaccagcagg tcgataattt
    10681 tcagaccaaa gtcgatagct gtacccggcc cctggctggt cagcaatttt actcgtgcat
    10741 cccagacgac gcgcttgtcc tgccattgtt cggcgggaat tttgtctttc agcgtcggga
    10801 agccggtcat attgccaatc gggaagatat cgtgcgggac cagcacggtg gctggcgcgg
    10861 cgcaaatagc cgcgacaata cgccctgaac ggtggaactg tttaacggtt tcgacaagca
    10921 gggtgctatc gcgaaaacac tctgcgccct tgatgccacc aggcagcaca atcacgtcat
    10981 attcgccatc agccacttcg accagcggcg catccgccag cagcttaacg ccgcgcgagc
    11041 aggtaatcgc caggttacca tcgctggcga cgctggcagt ggtgactttg ataccgccgc
    11101 gaaccagcag atcgatagtg gtgacggctt cagtctcttc actaccaggg gcgaggcaaa
    11161 ccagtgccga tgcgctcata ttcactctcc tttcttttta ccatttcaaa caggcgggtg
    11221 ttttccggta cggcaatccc atgcgcgcgg gcgcggcgta agagaaaacc attgatatag
    11281 tcgatttcgg tgtggcgcag cgcgcggata tcctgcaaca tcgacgagat attttccgct
    11341 gtggcatcaa tcacctgcat cacgtaatca cgcaaatctt ctgctgaagt atgatgccct
    11401 tcgcgttcga tcactgccgc gacttcttcg catatctgca taatttcttg cggatgatga
    11461 cgtaattcac cattcgggca attccaggtg gcggtcagtg gattaatcac acagttgact
    11521 gccagcttgc gccacagctc ggcgcgaata ttgttatgcc acgcgacgtc aggcaatacg
    11581 gtttgcaaaa tatccgccag ataactgtaa tcgccgtcct gttgccgtgc cggaccaata
    11641 tgcgtgatac cgtttgccac atgaataatg acattgccgt cgcggcgggc ggcatgggtg
    11701 gtggtgccca tcagtaatgg ctgctgaatg ttttgcaact cttcgatggt gcccatgccg
    11761 ttgtgaatta acagtattgg cgtagttaca ggcagtgtgg acgcgaggct tttgacggca
    11821 tcggaaacct gccatgcttt cagcgtcacc aggagcagat cgctggtggc aagaaaatcg
    11881 ggatcgttag cggtcagcga ttcgttaaat atcgaaccat cagtctcaac cagattcaca
    11941 ctacaataag gttgcggtac gcgcagccag ccctgaactt catgaccctg tttgcaaagt
    12001 gctgtaagcc ataattgccc taaggcaccg catcccaata cggtaatttt cattgttcct
    12061 cctcacccgc aaccactccg ggtgttcaat aaggctatct cttaattgtg catgctgttg
    12121 cgactatgca caattaaggg atacgtcctg gtgcaggact gttggttatt taactttgca
    12181 ggtattatgc ttcgcatcaa aaatgaaggg agaggaaaag atgccatctt tcgatattgt
    12241 ctctgaagtt gatcttcagg aagcacgtaa cgcggtcgat aacgcgagcc gcgaagtgga
    12301 gtcccgtttt gacttccgta atgttgaagc ctcatttgag ctgaacgacg ccagcaaaac
    12361 catcaaagtg ttgagcgagt ccgatttcca ggtcaatcag ttgctggata ttctgcgtgc
    12421 caagctgctg aagcgcggca ttgaaggcag ttcactggat gtaccggaaa atatcgttca
    12481 tagcggtaaa acctggtttg tggaagcgaa actgaaacag ggcattgaga gcgcgaccca
    12541 gaagaaaatc gtcaagatga ttaaagacag caaactgaaa gtgcaggcgc aaattcaggg
    12601 cgatgagatc cgtgtaacgg gtaaatctcg tgatgatttg caggctgtta tggcgatggt
    12661 acgtgggggc gatctcggtc agccgttcca gttcaaaaac ttccgcgatt aatcgcgaca
    12721 acctgcgctt tgttcatgtc agatgcggca tgaacgcctg atttggccta caaaggttta
    12781 taactcagga actttgtagg cctggtaagc gtagcgcagc aggcaatttc gcctcgcttt
    12841 tatgcctggc gaattgcctg ttcaacctca aagcgattcg tcactttgct gtcgattttc
    12901 acatacgctg aatgttcttc ttctgcaatc aacacttctt tgacgccttc agtttccagc
    12961 aaacgtactt ttaacgcttc gtttgcggca atgtccgccg ggatttcaat gcgcaagctg
    13021 ctgacatacg gcggttcttt catggtactg gcgactgcca gccacactgc ggccagcatt
    13081 gcgccagcga gaaatacgcc ctgaccgtca aacatgccgt caatccagcc gcccagcgaa
    13141 ccgccaatcg ccacgccaag aaactggctg gtggagtaaa cacccatcgc cgtacctttg
    13201 taacctgcag gcgactcttt actgataagt gaaggcagaa gggcttccat caaattaaac
    13261 gctacaaaga aaagctgcac gccgaccacc agttgccaga actgcgtttg tgcgttccac
    13321 aacacaattt ccgcaaccac gatcaatccg acgcagaaga caaagacttg cttcattttg
    13381 cgcttaactt cagcgtagat aatgaaaggc acgaccgagc caaaggcgat tagcatcgtc
    13441 gccagataga ccttccagtg ttcagccgcc gggaaccccg catcagccag ctgtccgggc
    13501 agggcaacaa acgtagacat cagaagcatg tgtagacaca taatgccaaa gttgagtttc
    13561 agcaaccgcg gttccgccag cactttactg aaactgcctt tcaccattcc ggactcacga
    13621 ttaagtacgt gagtgctact gttgggcaca acccaaatgg tcaacgcaat gccggttgtt
    13681 gccagaatag cgatcatcca gaacagcgcg tgcagcccaa gtttgtgagt gatgatcggg
    13741 ccaagcacca tcgcaatggc aaaggtaatg ccaaagctca cgccgataaa cgccatcgct
    13801 ttggtgcggt tttgttcgcg cgtaagatcg gaaagcagcg ccataacggc agcggcaatc
    13861 gcaccggagc cttgtagcgc ccggcccaga ataattcccc agatggagtc agagagcgca
    13921 gcgataacgc taccggcggc aaacaccgcc agcccaccga caattaatgg tttgcgacca
    13981 atgcggtctg aaagcaggcc aaacggaatc tgaaaaacgg cctgagtcag accataaata
    14041 ccaatggcaa taccgattaa tgcttcgctg gcaccttgca gagccatgcc gtacgtggtc
    14101 agaaccggca gaaccatgaa catgcccagc atgcgcaacg agaaaacggt gcctaaaccc
    14161 caggtcgcgc gccgctcacc tggcgtcatt ttataatcgt tcattaccac ctcttaaaaa
    14221 aatgcccaca tagtgtagtg cggagcgcat gttgggtaaa cagagtgtta atgagaaaat
    14281 attacagagg agaatagttt gtgtgtcagg tatgtttatt ttgcattcat aaaatgaatg
    14341 ctctatatgt tgaaaaaatt attcttaaag ctaaatagtt ttacatcaaa agtaataata
    14401 ttatttcttg attgctatgt gcatttttta tcttgcgaac atgctatttt tatctcacta
    14461 aataaacgat ttggtgacat cgatgataat gtttcaggtt aaatgtgtgc ttattacttg
    14521 ggcctttatg gtactgtatt tattcattaa atggatgttt ggtggttaat tattatggaa
    14581 atatatgaca gagagaacat aaaggtatac atgttaacta aaagaatatt attttcttta
    14641 atgctaatag tttcaccatg cgtggtggca agcgagaaag cgcatgaatt gtatgacagc
    14701 atatacggtg gtcaacctgc gcccgatgtt ataaatagac tgcataaaat ggctgaattg
    14761 ggagatgtcg acgcgcagag cctgttaggc tgggaatatt atcagcctcg ctttgatacc
    14821 aaacctgatg ttcaggaagc gattaaatgg tttgagttag ccgctaaaca cggtgataga
    14881 gaagctccgt tagcgctggg gagtatctac tacgacggtg agctggtgcg ggtggattat
    14941 gccaaagcgt atgcactatt taatcaggcc gcgcaatacg gtgtgaattt agcatggtcc
    15001 aggttgggca tgatgtacgc caatggtcag tatgttgagg tagattgtaa gaaagcgaag
    15061 gaatatcttg ataaaggtgt ccacatttat ggtggtccgg aagactttct ggctacatgt
    15121 cgaaaagaca tgattgacag aaaaacggtt gacgatacgt tacccgtgat cacggttacc
    15181 cgttctggga tgcgggataa ttttttagat aaaggttttt cctgcatgga tagcctcttc
    15241 gccaccacca ataaattagg cgaagtggcc aatcttcgcg tgacattcag cattcgtagc
    15301 ccatccggta aggagattaa ccaaacggtt ggcttcgcgc ctttcggttt aaaccggctg
    15361 aatattagct ttactgatta catatttgat tcatttacca gtaattcgtc tctaattctt
    15421 tataaaccgg aatttgagcg aaaatcttgt gcaactgtaa ggaccacaat cgttgccgca
    15481 acggcaacca ttaacggcaa ggatgtggag ttgctgaaag cgggggcaat tgaacaaaag
    15541 tggtaacgct gctcagtccc cggctgaggc aaaactagcc ctggccggag gcgataataa
    15601 tctaagccgc gagaatatgc tgaatcgcta aaaacatcgg tacagcaccg aaaacattgg
    15661 gcctcgctga aagctgaaca aagccgaggc gagtataaaa gttaatggcg gcagggtcag
    15721 catcaagata aaccccttta attggtaatg cccggtgaat tatttttaca tgttcaaaaa
    15781 aatcacatag taggtcctga ccaaagcccc gcttttgata tttccgcgct acccctaaca
    15841 tgactaatct gacaacacca atctctgaag gttgtgaacc ctgaaggacg ccagaaacgc
    15901 gttgtttttc cagcgaatat gccgtaaaag tacagatgcc aatcagttca ccactttgtc
    15961 tgtcaataag agctttagca gcacagtcgc tattacgcac acttttcttt agcgatgcgc
    16021 gtacaaattt atcgataaca gggttaccgc aatcaaactg tttttgccca ggataagtaa
    16081 tatcagactg ataattacag gagaggacta tgggcagagt aatctcttca tgcttgtcga
    16141 ccatcgctgt ttttcctttt cattaacgcc ttgagggcga gcgtcggttg agcaggttca
    16201 gcgatgagtt ggttcatcag ttcccagctt tgattcgaaa gctcacggcg acgttggtta
    16261 tcaagcacac tttcggcgcg ttccatagct gcgctcaaaa taaaagcgct taaatctacc
    16321 ccaacggctt gcgcagcttc acgaagtttg tttttggcgt cggggctggt tttgagttca
    16381 acccgcgcat ttttcagcgt tttatcgata tcgggtagag cgactgtttt tccaggcatt
    16441 atgacctctt tgattaactg aatgaaatcg tagggagtcg atctcatgca taatacggct
    16501 atcaaccgtg tgattaattt atgtgcatat gctcactctg tcaagagata cggttattag
    16561 ccgtatctct gatctcagac aataaaaaag gtgccgtagc acctttttta tagagaggtt
    16621 gtattaccac acagcagcca gcagcgtatg cgagtccggc accataaaat caacggacat
    16681 catcaccgag agggcagtga tggcgatgat agagaagccg aacagcttgc gcgcccagat
    16741 tcgatcatca gcaactttat aaccgcgcag ggccatacct aaccaccaga cgctcaccgc
    16801 cgcggcgacc accagatatt tatacccagc gtaaccgcca agagagagca tcagcgtggc
    16861 aacggcaaag gcgatgatat acagcgtgat gtgattcttc gccaccgaaa tgcctttcac
    16921 caccggcagt accggaatgt ttgcggcctg ataatcctta aagcggaaaa tggcgatggc
    16981 ataagaatga ggcatctgcc acaggctgaa gatagccagc aggattgctg cgccgctatc
    17041 gaactcgccg gttaccgcac agtagccgat cactggcggt gctgcgccgg agagcgaacc
    17101 aatcaatgtg ccgtagaccg agtggcgttt catgtacagg ctgtagacgc cgacataaac
    17161 cacaaagccc atcaccccca gccagcaggc cagcggattc gcgccaaacc acagcagcat
    17221 aaagccagca atacccagca aggtggcgta caccagcgag acagcaggag agatcaggcc
    17281 tttaaccagc acccgattct tcgtcctttc catctttctg tcgatatccc tgtcgatgta
    17341 gttgttaaac acacaacccg acgccacaac cagtgacacc ccaaccagcg tgtagataaa
    17401 caggggataa tcaatgctgc cctttgaggc cagcaggaat cccccaatca ccgagatcag
    17461 gttgccaaag atgatgcctg gtttcgttac ttgcaggtat tgcttaaaca tcataaccgc
    17521 cgctcttagt gcatcatcat gttgtagttg aggttccaca taatccagat ggagcctaca
    17581 accaggatag cgatgattag cacggtgaag acaaacgccg tcatgttcca gccttcatct
    17641 gatttggtat tcatgtgcag gaagcacacc agatgcacca gaacctgtac cactgccatt
    17701 gccaggattg ttcccagaat tacggccgga gaggcagctc ctgtcatcac catccagaac
    17761 ggaatcaccg tcagaatgat cgacaggata aagcctgtca tgtaggtttt tacgctgcca
    17821 tgggacgcgc cgctgtgatc ggtagaatga ctcattacat cgcccccatc agataaacaa
    17881 cagtgaacac acagatccaa accacatcca ggaagtgcca gaacaggctc aggcacatga
    17941 tgcgggtacg gttagtgctg gtcaggccgc gacgggcgat ttgcaccatc agcaccgcca
    18001 tccagataag accggaagtg acgtgcagac cgtgcgtgcc gaccagcgca aagaacgctg
    18061 acaggaagcc gctgcgatcc ggacccatgc cgttaacaat caggtgatgg aattcataga
    18121 tttccatccc gataaatccg gcaccaaaca accaggtcaa cgccagccag gagataacct
    18181 ggcttttgtt gtttttgtac atggcgatag ccgccatgcc gtaggtgatg gagctgaaca
    18241 acagcaagaa agtttcaacc agaacgaacg gcagttcgaa aatgtcctta cctgtcgggc
    18301 cgcctgcggt gccgttaacc agaacggcat aggtagcaaa caagatagag aacagaatgc
    18361 agtcgctcat caggtagatc caaaatccga agattttggt tccgcctgca tcgtggtgcc
    18421 cgtgttcgtg cgcgtgggca gtcgcgtgcg tcaaagtatc agttgccatt tttcagccct
    18481 gccttagtaa tctcatcgaa atgctggttt tccagttttt cgatttctgc caccggcacg
    18541 tagtaatcca cgtcctcgtc gaagcttttc acgatccagg tgatgatcat gcctgcgaag
    18601 ccaacaatcg ccagccacca gatatgccag atcatggcga aaccgaagat ggtggagaaa
    18661 gccgcaatga cgataccggc accgctgttt ttcggcatat gaatttcttc atagtggtca
    18721 ggctttttgt acgcttcgcc tttctctttc atttcccaga atgcatcacg ttcgtgaacg
    18781 tgcggcacaa cggcaaagtt atagaacgga ggcggggaag aggttgccca ctccagcgta
    18841 cggccacccc acgggtcgcc agtcaggtca cggttctggt cgcggtcacg aatagaaacg
    18901 tacatctgaa taacgaggca gagaataccc agcgcaatca gtaccgcacc gctggctgca
    18961 atcatcagca tggtgtggaa ctgcgggtca atctgctggc tcaaacgacg ggtcatgccc
    19021 atgaagccca gcgcatacag tggcataaag gcaacgaaga agccgatgat ccagaaccag
    19081 aacgcgcgtt taccccaggt ttcgttcagt ttgaaaccga acgctttagg ccaccagtag
    19141 gtcatccctg cgaagcagcc gaagaccacg ccgccgatga tcacgttatg gaagtgcgca
    19201 atcaggaaca ggctgttatg cagaacgaag tccgcgcccg gtactgccag cagcacgcca
    19261 gtcatcccgc ccaccgagaa ggtgacgata aaaccgatgg tccacagcat cgcagaatgg
    19321 aacacgatgc ggccctgata catggtgaac agccagttga agatcttcac cccggtcggg
    19381 atggcgataa tcatggtggt gataccaaag aaggcgttta cgttcgcgcc cgcacccatc
    19441 gtaaagaagt ggtgcagcca gacgatgaac gacagcacgg tgatacagac ggttgcccat
    19501 accagcgagg tataaccaaa cagacgttta cgcgagaagg ttgccgcaat ttcggagaac
    19561 acaccgaaaa caggcaggat caggatgtaa acttccgggt ggccccaggc ccaaatcagg
    19621 ttgatgtaca tcatcatgtt gccacccata tcgttggtaa agaaatgggt gcccagatag
    19681 cgatccaggg tcaacaacgc aacggtaacc gtcagaattg ggaaggaagc aataatcagt
    19741 acgttcgcgc acagtgatgc ccaggtaaat actggcatct tgaacatggt catgcccggt
    19801 gcgcgcatct tcagaatggt aacgaagaag ttgataccgg taagcgtcgt acctataccg
    19861 gatagctgga gactccatat ccagtaatcg acaccgactc ccggactgta ctctattccc
    19921 gatagcggtg gataggccag ccagccggtc tgcgcaaatt cgcccacgcc gagagaaacg
    19981 ttgaccagaa tcacaccaac aacggtaaac cagaagctta agttgttgag gaacgggaac
    20041 gcaacgtcac gcgcgccgat ctgcagcgga accaccaggt tcatcagacc gataacgaaa
    20101 ggcatcgcca cgaagaagat cataatcacg ccgtgcgcgg tgaagatctg atcgtagtgg
    20161 tgaggaggca ggaagcccgc ttcgcccgcc gaggc
//
LOCUS       DAMZFT010000086        20122 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.086, whole genome shotgun sequence.
ACCESSION   DAMZFT010000086 DAMZFT010000000
VERSION     DAMZFT010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20122)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20122)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20122
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.086"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..476
                     /locus_tag="PN977_003270"
     CDS             <1..476
                     /locus_tag="PN977_003270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HDI6043275.1"
                     /translation="EAADRLRDEPLIFAIVGQGGGKARLEKMAQQRGLRNMQFFPLQS
                     YDALPALLKMGDCHLVVQKRGAADAVLPSKLTNILAVGGNAVITAEAHTELGQLCETF
                     PGIAVCVEPESVEALVAGIRQALLLPKHNTVAREYAERTLDKENVLRQFINDIRG"
     gene            479..1915
                     /gene="cpsB"
                     /locus_tag="PN977_003271"
     CDS             479..1915
                     /gene="cpsB"
                     /locus_tag="PN977_003271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanyltransferase"
                     /protein_id="HDI6043276.1"
                     /translation="MAQSKLYPVVMAGGSGSRLWPLSRVLYPKQFLCLKGDLTMLQTT
                     ICRLNGVECESPVVICNEQHRFIVAEQLRQLNKLTENIILEPAGRNTAPAIALAALAA
                     KRHSPENDPLMLVLAADHVIADEDAFRAAVRNAMPYAEAGKLVTFGIVPDLPETGYGY
                     IRRGEVSAGEQDAVAFEVAQFVEKPNLETAQAYVASGEYYWNSGMFLFRAGRYLEELK
                     KYRPDILDACEKAMNAVDPDLDFIRVDEEAFLACPEESVDYAVMERTADAVVVPMDAG
                     WSDVGSWSSLWEISAHTAEGNVCHGDVINHKTENSYVYAESGLVTTVGVKDLVVVQTK
                     DAVLIADRNAVQDVKKVVEQIKADGRHEHRVHREVYRPWGKYDSIDAGDRYQVKRITV
                     KPGEGLSVQMHHHRAEHWVVVAGTAKVTIDGDIKLLGENESIYIPLGATHCLENPGKI
                     PLDLIEVRSGSYLEEDDVVRFADRYGRV"
     gene            2017..3387
                     /gene="cpsG"
                     /locus_tag="PN977_003272"
                     /gene_synonym="manB"
     CDS             2017..3387
                     /gene="cpsG"
                     /locus_tag="PN977_003272"
                     /gene_synonym="manB"
                     /EC_number="5.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis phosphomannomutase
                     CpsG"
                     /protein_id="HDI6043277.1"
                     /translation="MKKLTCFKAYDIRGKLGEELNEDIAWRIGRAYGEFLKPKTIVLG
                     GDVRLTSETLKLALAKGLQDAGVDVLDIGMSGTEEIYFATFHLGVDGGIEVTASHNPM
                     DYNGMKLVREGARPISGDTGLRDVQRLAEANDFPPVDETKRGRYQQINLRDAYVDHLF
                     GYINVKNLTPLKLVINSGNGAAGPVVDAIEARFKALGAPVELIKVHNTPDGNFPNGIP
                     NPLLPECRDDTRNAVIKHGADMGIAFDGDFDRCFLFDEKGQFIEGYYIVGLLAEAFLE
                     KNPGAKIIHDPRLSWNTVDVVTAAGGTPVMSKTGHAFIKERMRKEDAIYGGEMSAHHY
                     FRDFAYCDSGMIPWLLVAELVCLKGKTLGELVRDRMAAFPASGEINSKLAQPVEAINR
                     VEQHFSREALAVDRTDGISMTFADWRFNLRSSNTEPVVRLNVESRGDVPLMEARTRTL
                     LTLLNE"
     gene            3442..4836
                     /gene="wcaJ"
                     /locus_tag="PN977_003273"
     CDS             3442..4836
                     /gene="wcaJ"
                     /locus_tag="PN977_003273"
                     /EC_number="2.7.8.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate glucose
                     phosphotransferase"
                     /protein_id="HDI6043278.1"
                     /translation="MTNLKKRERAKTNASLISMVQRFSDITIMFAGLWLVCEVSGLSF
                     LYMHLLVALITLVVFQMLGGITDFYRSWRGVRAATEFALLLQNWTLSVIFSAGLVAFN
                     NDFDTQLKIWLAWYGLTSIGLVVCRSCIRIGAGWLRNHGYNKRMVAVAGDLAAGQMLM
                     ESFRNQPWLGFEVVGVYHDPKPGGVSNDWAGNLQQLVEDAKAGKIHNVYIAMQMCDGA
                     RVKKLVHQLADTTCSVLLIPDVFTFNILHSRLEEMNGVPVVPLYDTPLSGVNRLIKRA
                     EDIVLATLILLLISPVLCCIALAVKLSSPGPVIFRQTRYGMDGKPIKVWKFRSMKVME
                     NDKVVTQATQNDPRVTKVGNFLRRTSLDELPQFINVLTGGMSIVGPRPHAVAHNEQYR
                     QLIEGYMLRHKVKPGITGWAQINGWRGETDTLEKMEKRVEFDLEYIREWSVWFDIKIV
                     FLTVFKGFVNKAAY"
     gene            4838..6316
                     /gene="wzxC"
                     /locus_tag="PN977_003274"
     CDS             4838..6316
                     /gene="wzxC"
                     /locus_tag="PN977_003274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid undecaprenyl disphosphate flippase
                     WzxC"
                     /protein_id="HDI6043279.1"
                     /translation="MSLREKTISGAKWSAIATVIIIGLGLVQMTVLARIIDNHQFGLL
                     TVSLVIIALADTLSDFGIANSIIQRKEISHLELTTLYWLNVGLGLVVCVAVFLLSDLI
                     GDVLNNPDLAPLIKTLSLAFVVIPHGQQFRALMQKELEFNKIGMIETSAVLAGFTFTV
                     VSAHFWPLAMTAILGYLVNSAVRTLLFGYFGRKIYRPGLHFSLASVAPNLRFGAWLTA
                     DSIINYLNTNLSTLVLARILGAGVAGGYNLAYNVAVVPPMKLNPIITRVLFPAFAKIQ
                     DDTEKLRVNFYKLLSVVGIINFPALLGLMVVSNNFVPLVFGEKWNSIIPVLQLLCVVG
                     LLRSVGNPIGSLLMAKARVDISFKFNVFKTFLFIPAIVIGGQMAGAIGVTLGFLLVQI
                     INTILSYFVMIKPVLGSSYRQYILSLWLPFYLSLPTLGVSYALGIVLKGQLALGMLLA
                     VQIAAGVLAFVVMIVLSRHSLVVEVKRQFCRSEKMKMLLRAG"
     gene            6388..7668
                     /gene="wcaK"
                     /locus_tag="PN977_003275"
     CDS             6388..7668
                     /gene="wcaK"
                     /locus_tag="PN977_003275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis pyruvyl transferase
                     WcaK"
                     /protein_id="HDI6043280.1"
                     /translation="MKLLILGNHTCGNRGDSAILRGLLDAINILNPHAEVDVMSRYPV
                     SSSWLLNRPVMGDPLFLQMKQHNSAAGVVGRVKKVLRRRYQHQVLLSRVTDTGKLRNI
                     AIAQGFTDFVRLLSGYDAIIQVGGSFFVDLYGVPQFEHALCTFMAKKPLFMIGHSVGP
                     FQDEQFNQLANYVFGHCDALILRESVSLDLMKRSNITTAKVEHGVDTAWLVDHHTEDF
                     TASYAVQHWLDVAAQQKTVAITLRELAPFDKRLGTTQQAYEKAFAGVVNRILDEGYQV
                     IALSTCTGIDSYNKDDRMVALNLRQHISDPARYHVVMDELNDLEMGKILGACELTVGT
                     RLHSAIISMNFATPAIAINYEHKSAGIMQQLGLPEMAIDIRHLLDGSLQAMVADTLGQ
                     LPALNARLNEAVSRERHTGMQMVQSVLERIGEVK"
     gene            7665..8885
                     /gene="wcaL"
                     /locus_tag="PN977_003276"
     CDS             7665..8885
                     /gene="wcaL"
                     /locus_tag="PN977_003276"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaL"
                     /protein_id="HDI6043281.1"
                     /translation="MKVGFFLLKFPLSSETFVLNQITAFIDMGFEVEIVALQKGDTQN
                     THAAWTKYNLAARTRWLQDEPTGKVAKLRHRASQTLRGIHRKNTWQALNLKRYGAESR
                     NLILSAICGQVATPFRADVFIAHFGPAGVTAAKLRELGVIRGKIATIFHGIDISSRDV
                     LNHYTPEYQQLFRRGDLMLPISDLWAGRLQKMGCPREKIAVSRMGVDMTRFSPRPVKA
                     PATPLEIISVARLTEKKGLHVAIEACRQLKEQGVAFRYRILGIGPWERRLRTLIEQYQ
                     LEDVVEMPGFKPSHEVKAMLDDADVFLLPSITGADGDMEGIPVALMEAMAVGIPVVST
                     LHSGIPELVEADKSGWLVPENDARALAQRLAAFSQLNTDELAPVVKRAREKVEHDFNQ
                     QVINRELASLLQAL"
     gene            8896..10290
                     /gene="wcaM"
                     /locus_tag="PN977_003277"
     CDS             8896..10290
                     /gene="wcaM"
                     /locus_tag="PN977_003277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis protein WcaM"
                     /protein_id="HDI6043282.1"
                     /translation="MPFKKLSRRTFLTASSALAFLHTPFARALPARQSVNINDYNPHD
                     WIASFKQAFGEGQTVVVPAGLVCDNINTGIFIPAGKTLHILGSLRGNGRGRFVLQDSS
                     QVTGEEGGSMHNITLDVRGSDCTIKGLTMSGFGPVTQIYIGGKNKRVMRNLSIDNLTV
                     SHANYAILRQGFHNQIIGANITNCKFSDLQGDAIEWNVAINDRDILISDHVIERINCT
                     NGKINWGIGIGLAGSTYDNNYPEDQAVKNFVVANITGSDCRQLIHVENGKHFVIRNIK
                     ARNITPDFSKKAGIDNATVAIYGCDNFVIDNIEMINSAGMLIGYGVIKGKYLSIPQNF
                     RVNNIQMDNTHLAYKLRGIQISAGNAVSFVALTNIEMKRASLELHNKPQHLFMRNIKV
                     MQESSVGPALSMNFDMRKDVRGVFMAKKETLLSLANVHAVNEKGQSSVDIDRVNHHIV
                     NVEKINFRLPERRE"
     gene            10448..11443
                     /locus_tag="PN977_003278"
     CDS             10448..11443
                     /locus_tag="PN977_003278"
                     /EC_number="5.1.3.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-alpha-D-glucosaminyl-diphospho-ditrans,
                     octacis-undecaprenol 4-epimerase"
                     /protein_id="HDI6043283.1"
                     /translation="MNDNVLLIGASGFVGTRLLETAIADFNIKNLDKQQSHFYPEITQ
                     IGDVRDQQALDQALAGFDTVVLLAAEHRDDVSPTSLYYDVNVQGTRNVLAAMEKNGVK
                     NIIFTSSVAVYGLNKHNPDENHPHDPFNHYGKSKWQAEEVLREWYNKAPTERSLTIIR
                     PTVIFGERNRGNVYNLLKQIAGGKFMMVGAGTNYKSMAYVGNIVEFIKYKLKNVAAGY
                     EVYNYVDKPDLNMNQLVAEVEQSLNKKIPSMHLPYPLGMLGGYCFDILSKITGKKYAV
                     SSVRVKKFCATTQFDATKVHSSGFVAPYTLSQGLDRTLQYEFVHAKKDDITFVSE"
     gene            11686..12579
                     /gene="galF"
                     /locus_tag="PN977_003279"
     CDS             11686..12579
                     /gene="galF"
                     /locus_tag="PN977_003279"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalF"
                     /protein_id="HDI6043284.1"
                     /translation="MTNLKAVIPVAGLGMHMLPATKAIPKEMLPIVDKPMIQYIVDEI
                     VAAGIKEILLVTHASKNAVENHFDTSYELESLLEQRVKRQLLAEVQSICPPGVTIMNV
                     RQGEPLGLGHSILCARPAIGDNPFVVVLPDVVIDDASADPLRYNLAAMIARFNETGRS
                     QVLAKRMPGDLSEYSVIQTKEPLDREGKVSRIVEFIEKPDQPQTLDSDIMAVGRYVLS
                     ADIWPELERTQPGAWGRIQLTDAIAELAKKQSVDAMLMTGDSYDCGKKMGYMQAFVKY
                     GLRNLKEGAKFRKGIEKLLSE"
     gene            12796..13710
                     /locus_tag="PN977_003280"
     CDS             12796..13710
                     /locus_tag="PN977_003280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001183563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HDI6043285.1"
                     /translation="MQQCLSVTVRNINEFSALVAVKPGAVAWIDGMKNKCPRIAVIMS
                     VYINDKFNEFKEAFDSIINQTYSNFVFFIYADGKINPDIESFLQNYNSQHVIFIRSEK
                     NKGLAYALNKLISVALVNNEFDFIARMDADDISLEDRFQRQIDFFMANPELDVVGGLC
                     QEFGGESAREKCKIYFTDEAIKNNAFKKCPFVHPTVMFKSSVFNNGMRYPENQPLTED
                     MCLWLELISANKKFANIDSVLLKYRINEQTLKRRRGLNKAVTELSTRLYYAKKLKMLS
                     VRNLLYILIHFGVRVLPSNIFKLVHKLN"
     gene            13712..15478
                     /locus_tag="PN977_003281"
     CDS             13712..15478
                     /locus_tag="PN977_003281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001373134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6043286.1"
                     /translation="MNKTVYTYDVWDTILKRHCLPSYTLEVSLYWLLLYLGKPEKHKD
                     VYKKVKALESDFFNRKEEYFIEELIVGILIENNLMNGFCNNSVTAAWEQIYYFVERNC
                     TYKNKEVIELIGSDYGDKLFISDFYTDSSFIKKLLHHHQVSVLDGVTSAEYGVSKHMG
                     SLYEKIELLKQRKWIHTGDNELADIKMAKRAGAQVRLIKSKKKKINKLKIKNDYERLA
                     FIILSFSLFILKTSRKKKCNKIYFFTREGVFFKKNFDLLLNQIPNIFPSITTEILPIS
                     RVASLALKFNESDEFLGFNDALTQYGYGVSTFLSFFHLDDCYSELTTKYHDVNDLLSH
                     RNDPLVKQLIKSIEDKKKRTENFLTSIEFDSEPSIIVDIGWRGSIQDNLMSRKNNYIQ
                     HGCYLGLFDFYPGQSGQNKSSVIFDNNRFRQKWTMKGVAFMETVFNALDGSVVDYVNN
                     KPKRKENYAEIENSKHLIVMQDAIIEEFMNLSIMLKEGKLNINDIERLAIESYKKIVT
                     NPSKEIADFYVGSIQNESFGLNDFIDKEFNISAVDVFRSFFSKSKRNEIRIKLHRNGW
                     RESILKSSKVSLSAKLCSLLIR"
     gene            15482..16354
                     /locus_tag="PN977_003282"
     CDS             15482..16354
                     /locus_tag="PN977_003282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001027535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HDI6043287.1"
                     /translation="MNISVIICCYNSENRIVNTLFHLSQCVSLSKGLIDEIILVDNNS
                     SDETVKTAEDFWRNTGEEQIFKIVFESIPGLVHARRRGLDESKNEIVVFCDDDNWLVP
                     SYFTKLEKYFENKQVGAVGGRGIPIANTVIPEWFFENLDAYACSAQTSNMLYGASLAI
                     RSSILKKFYTDALSVKLKGRTKDSLDSGEDNLLCNFVKKQGYTLIPDNTPFCHFMESK
                     RLDVDYLYKLAESFGKATASMKNNSFTTDYVYLTAKMLLAISRIFSKKNKKIYLKREI
                     SFLRGYLRFRKESK"
     gene            16354..17532
                     /locus_tag="PN977_003283"
     CDS             16354..17532
                     /locus_tag="PN977_003283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001483632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide repeat unit polymerase"
                     /protein_id="HDI6043288.1"
                     /translation="MRGGMLVIAWYSIFLLISATGMTGLNKLSTEAILCYIAFLFCFF
                     FGMMLGGVSLPNGMKMLQQEQKIKKRHASAIVLIYCFFIFYSLIALLNIYYFNKVDLS
                     SYRQAFFDGSRDNVKYFYGVFGFYFYYLLSLLVFALVPYTIITNSKKTISLCFFALLL
                     YDIIFLSRTGVYYFILSYIVSSILLKKGIKKIIIVGMVALLFSLIMSYTRDMKSDIIG
                     TLFSSIINYHIAPFVLFDENIISNHLIKYNGAGLASLGIYNIIFYPIDSSIMSSINDF
                     RAQLNEFYDLGVNRYLPYNAYYTSLGGVYIDSGLIGSMMVSFMTGFIIVGIEKSAFKS
                     TKFLVSSVFFTTLCMESIFSPITLNIFAFTIIVYLVVLMVLKYENSDDNTIFKKYRAK
                     "
     gene            17483..18508
                     /locus_tag="PN977_003284"
     CDS             17483..18508
                     /locus_tag="PN977_003284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001328290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HDI6043289.1"
                     /translation="MKIAMIIPSLKNTAPNNIALSICEGARKHGVNIDIYYLDEIVEL
                     DIDESELNINRLNDRTILNSYDIVHSHGLRPDVINCKTSKNVINVSTLHSFILDDLKN
                     KYGIKGYLIAFAWLKLLKRFDSCIAISEATKRFYVKHGLKCHHIYNGIDFPDIELYQD
                     NSRLNNGKINIVSIAHLEKIKGLEQLLYLAAEREEFHVHIIGEGTYRGKLTNIIDKFD
                     LSQRVTLHGYISNASSMLGKFDVYVQPSKSEGFGIAVIEALVNKIPTVCSDIEVFREL
                     FGSGEVEFFKLDDINSLYDAISSALTKTDMSRDASATAISQKFSSEVMSLNYLSWYKK
                     LYEERNL"
     gene            18489..19667
                     /locus_tag="PN977_003285"
     CDS             18489..19667
                     /locus_tag="PN977_003285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HDI6043290.1"
                     /translation="MKKEIYKYGTIILCYGINFILPILFFKEISLILSPIVLSSFFFV
                     LALLMYCQFIVEYGLQISLLRKLNENKSDLSRLLSTILGLKVILFVLCAVFIYCILLY
                     NNEVIFFILVFILLGNVFSCQFLYQVVDQLHFFYVLNSLVKLIFIPLIFINDNYIYLM
                     ICYSCFNILPNLAALAYFLYKNKVKVVKVPACEIYNLSKECFSYFISNISITLYTNFY
                     QILLGIISPAYLAAYALSDKIIRGIVSGNYVFIQIVQVQYLNDESKFINGTWKTIVSL
                     LLLGGMEFLFIFFGADFFSRYLFPTISLLPIFLKLMSLLIVVILLSNFFAMVYLPCSG
                     NTLFLSRVLIFVSIVSVVVAPILIWLYAGYGAVISAIFSELLVLILCYGLYLKLKHKL
                     "
BASE COUNT         5553 a         4139 c         4846 g         5584 t
ORIGIN      
        1 ttgaagctgc cgatcgcctg cgcgatgaac cgcttatttt tgccattgtc gggcagggcg
       61 gcggcaaagc gcggcttgaa aaaatggcgc agcagcgtgg actgcgcaac atgcaatttt
      121 tcccgctgca atcgtatgac gctttacccg cactgctgaa gatgggggat tgccatctgg
      181 tggtgcaaaa acgcggcgcg gcagatgccg tattgccgtc gaaactgacc aatattctgg
      241 cggtaggcgg taacgcggtg attactgctg aagcccacac agaactggga cagctttgcg
      301 aaacctttcc gggcattgcg gtttgcgtag aaccggaatc ggtcgaggcg ctggtggcgg
      361 ggattcgtca ggcgctcctg ctgcccaaac acaacacggt ggcacgtgaa tatgccgaac
      421 gcacgctcga taaagagaac gtgttacgtc aatttataaa tgatattcgg ggataattat
      481 ggcgcagtcg aaactctatc cagttgtgat ggcaggtggc tccggtagcc gcttatggcc
      541 gctttcccgc gtactttacc ccaagcagtt tttatgcctg aaaggcgatc tcaccatgct
      601 gcaaaccacc atctgccgcc tgaacggcgt ggagtgcgaa agcccggtgg tgatttgcaa
      661 tgagcagcac cgctttattg tcgcggaaca gctgcgtcaa ctgaacaaac tcaccgagaa
      721 cattattctc gaaccggcag ggcgtaacac tgcaccggcc attgcgctgg cggcgctggc
      781 ggcaaaacgt catagcccgg agaacgaccc attaatgctg gtgctggcgg cggatcatgt
      841 gattgccgat gaagacgcgt tccgtgccgc cgtgcgtaat gccatgccgt atgccgaagc
      901 gggcaagctg gtgaccttcg gcattgtgcc ggatctacct gaaaccggat atggctatat
      961 tcgtcgcggt gaagtgtcgg cgggtgagca ggatgcggtg gcctttgaag tggcgcagtt
     1021 tgtcgaaaaa ccgaatctgg aaaccgctca ggcctatgtg gcaagtggcg aatattactg
     1081 gaacagcggt atgttcctgt tccgtgccgg acgctatctc gaagaactga aaaagtatcg
     1141 cccggatatt ctcgatgcct gtgaaaaagc gatgaacgcc gtcgatccgg atctcgattt
     1201 tattcgtgtg gatgaagagg cgtttctcgc ctgtccggaa gagtcggtgg attacgcggt
     1261 catggaacgc acggcagatg ccgttgtggt gccgatggat gcgggctgga gcgatgtcgg
     1321 ttcctggtct tcattatggg agatcagcgc ccacaccgcc gagggcaacg tttgccatgg
     1381 cgatgtgatt aatcacaaaa ctgaaaacag ctatgtgtac gccgaatctg gcctggtcac
     1441 caccgtcggg gtgaaagatt tggtggtagt gcagaccaaa gatgcagtgc tgattgccga
     1501 ccgtaatgcg gtgcaggatg tgaagaaagt ggtcgagcag atcaaagctg atggtcgcca
     1561 tgagcatcgg gtgcatcgcg aagtgtatcg tccgtggggc aaatatgact ctatcgacgc
     1621 gggcgaccgc taccaggtga aacgcatcac cgtgaaaccg ggcgaaggct tgtcggtaca
     1681 gatgcatcat caccgcgcgg agcactgggt ggtggtcgcg ggaacggcaa aagtcaccat
     1741 tgacggtgat atcaaactgc ttggtgaaaa cgagtccatt tatattccgc tgggggcgac
     1801 gcactgcctg gaaaacccgg ggaaaattcc gctcgattta attgaagtgc gctccggctc
     1861 ttatctcgaa gaggatgatg tggtgcgctt cgcggatcgc tacggacggg tgtagcagtt
     1921 gaataatcaa ccggaataaa tctccggcaa ataacgacaa aaacaaaatt ggccgtttcg
     1981 gtcagggcca actattgcct gaaaaagggt aacgacatga aaaaattaac ctgctttaaa
     2041 gcctatgata ttcgcgggaa attaggcgaa gaactgaatg aagatattgc ctggcgcatt
     2101 ggtcgcgctt atggcgaatt tctcaaaccg aaaaccattg tgttaggcgg tgatgtccgc
     2161 ctcaccagcg aaaccttaaa actggcgctg gcgaaaggtt tacaggatgc gggcgtcgat
     2221 gtgctggata tcggtatgtc cggcaccgaa gagatctatt tcgccacgtt ccatctcggc
     2281 gtggatggcg gcatcgaagt taccgccagc cataatccga tggattacaa cggcatgaaa
     2341 ctggtgcgcg aaggggctcg cccgatcagc ggcgataccg gactgcgcga cgtccagcgt
     2401 ctggcagaag ccaacgactt tcctcccgtc gatgaaacca aacgcggtcg ctatcagcaa
     2461 atcaatctgc gtgacgctta cgttgatcac ctgttcggtt atatcaacgt caaaaacctt
     2521 acgccgctca agctggtgat taactccggg aacggcgcgg cgggtccggt ggtggacgcc
     2581 atcgaagccc gctttaaagc cctcggcgca ccggtggagt taatcaaagt gcataacacg
     2641 ccggacggca atttccccaa cggtattcct aacccgttgc tgccggaatg tcgcgacgac
     2701 acccgcaatg cggtcatcaa acacggcgcg gatatgggca ttgcctttga tggcgatttt
     2761 gaccgctgtt tcctgtttga cgaaaaaggg cagtttatcg agggttacta cattgtcggt
     2821 ttgctggcag aagcgttcct cgaaaaaaat cccggcgcga agatcatcca cgatccacgt
     2881 ctctcctgga acaccgttga tgtggtgact gccgcaggcg gtacgccggt aatgtcgaaa
     2941 accggacacg cctttattaa agaacggatg cgcaaggaag acgccatcta cggtggcgaa
     3001 atgagcgccc accactattt ccgtgatttc gcttactgcg acagcggcat gatcccgtgg
     3061 ctgctggtcg ccgaactggt gtgcctgaaa ggaaaaacgc tgggcgaact ggtgcgcgac
     3121 cggatggcag cgtttccggc aagcggtgag atcaacagca aactggcgca acccgttgag
     3181 gcgattaacc gcgtggaaca gcattttagc cgtgaggcgc tggcggtgga tcgcaccgat
     3241 ggcatcagca tgacctttgc cgactggcgc tttaacctgc gctcctccaa caccgaaccg
     3301 gtggtgcggt tgaatgtgga atcgcgcggt gatgtgccgc tgatggaagc gcgaacgcga
     3361 actctgctga cgttgctgaa cgagtaatgt cggatcttcc cttaccccac tgcgggtaag
     3421 gggctaataa caggaacaac gatgacaaat ctaaaaaagc gcgagcgagc gaaaaccaat
     3481 gcatcgttaa tctctatggt gcaacgcttt tcagatatca ccatcatgtt tgccggacta
     3541 tggctggttt gcgaagtcag cggactgtca ttcctctaca tgcacctgtt ggtggcactg
     3601 attacgctgg tggtgttcca gatgctgggc ggcatcaccg atttttatcg ctcatggcgc
     3661 ggtgttcggg cagcgacaga atttgccctg ttgctacaaa actggacctt aagcgtgatt
     3721 ttcagcgctg gactggtggc gttcaacaat gatttcgaca cgcaactgaa aatctggctg
     3781 gcgtggtatg ggctgaccag tatcggactg gtggtttgcc gttcatgtat tcgcattggg
     3841 gcgggctggc tgcgtaatca tggctataac aagcgcatgg tcgcggtggc gggggattta
     3901 gccgccggac aaatgctgat ggagagcttc cgtaatcagc cgtggttagg gtttgaagtg
     3961 gtgggcgtat accacgaccc aaaaccgggc ggcgtttcta acgactgggc gggcaatctg
     4021 caacaactgg tcgaggatgc gaaagcgggc aagattcata acgtctatat cgcgatgcag
     4081 atgtgcgacg gcgcgcgagt gaaaaaactg gtccatcaac tggcggacac cacctgttcg
     4141 gtgctgctga tccccgatgt ctttaccttc aacattctcc attcacgcct cgaagagatg
     4201 aatggcgtac cggtggtgcc gctgtatgac acgccacttt ccggggttaa ccgcctgata
     4261 aaacgtgcgg aagacattgt gctggcgacg cttatcctgc tgctgatctc tccggtgctg
     4321 tgctgtattg cgctggcggt gaaactcagt tcgccagggc cggttatttt ccgccagact
     4381 cgctacggca tggatggcaa accgatcaaa gtgtggaagt tccgttcgat gaaagtgatg
     4441 gagaacgaca aagtggtgac tcaggcgacg cagaacgatc cgcgcgtcac caaagtgggg
     4501 aactttctgc gccgcacctc gctggatgaa ttgccgcagt ttatcaatgt gctgaccggc
     4561 gggatgtcga ttgtcgggcc acgtccacac gcggtggcgc ataacgaaca gtatcgacag
     4621 ctcattgaag gctacatgct gcgccataag gtgaaaccgg gcattaccgg ctgggcgcag
     4681 attaacggct ggcgcggcga aaccgacacg ctggagaaaa tggaaaaacg cgtcgagttc
     4741 gaccttgagt acatccgcga atggagcgtc tggttcgata tcaaaatcgt tttcctgacg
     4801 gtattcaaag gtttcgttaa caaagcggca tattgatatg agcttacgtg aaaaaaccat
     4861 cagcggcgcg aagtggtcgg cgattgccac ggtaatcatt atcggcctcg ggctggtgca
     4921 gatgaccgtg ctggcgcgga ttatcgacaa ccaccagttc ggcctgctta ccgtgtcgct
     4981 ggtgattatc gcgctggcag atacgctttc tgacttcggt atcgctaact cgattattca
     5041 gcgaaaagaa atcagtcacc tggaacttac cacgttgtac tggctgaacg tcgggctggg
     5101 gcttgtggtg tgtgtggcgg tgtttttgtt gagtgatctc attggtgacg tgctgaacaa
     5161 cccggacctg gcaccgttga ttaaaacatt gtcgctggcg tttgtggtga tcccccacgg
     5221 gcagcagttc cgcgcgttga tgcaaaaaga gctggagttc aacaaaatcg gcatgatcga
     5281 aaccagcgcg gtgctggcgg gctttacttt tacggtggtt agcgcccatt tctggccact
     5341 ggcgatgacc gcgatcctcg gttatctggt caatagtgcg gtaagaacgc tgctgtttgg
     5401 ctactttggt cgcaaaattt atcgccccgg tctgcatttc tcgctggcgt cggtggcacc
     5461 gaacttacgc tttggtgcct ggctgacggc ggacagcatc atcaactatc tcaataccaa
     5521 cctttcaacg ctggtgctgg cgcgtattct cggcgcgggc gtggcagggg gatacaacct
     5581 ggcgtacaac gtggccgttg tgccgccgat gaagctgaac ccaatcatca cccgcgtgtt
     5641 gtttccggca tttgccaaaa ttcaggacga caccgaaaag ctgcgcgtta acttctacaa
     5701 gctgctgtcg gtagtgggga ttatcaactt tccggcgctg ctggggctga tggtggtgtc
     5761 gaataacttt gtaccgctgg tctttggtga gaagtggaac agcattattc cggtgctgca
     5821 attgctgtgt gtggtggggc tgctgcgctc agtggggaac ccgattggtt cgctgctgat
     5881 ggcgaaagcg cgggtcgata tcagctttaa attcaacgta ttcaaaacct ttctgtttat
     5941 tccggcgatt gttattggtg ggcagatggc gggcgcaatc ggcgtcacgc ttggcttcct
     6001 gctggtgcag attatcaaca ccattctgag ctatttcgtg atgattaaac cggtgctcgg
     6061 ttccagttat cgccagtaca tcctgagttt gtggctgccg ttttacctct cgctgccgac
     6121 gctgggggtc agttatgcgc tgggcattgt gctgaaaggg caactggcgc tggggatgct
     6181 gctggcggtg caaatagccg cgggcgtgct ggcgtttgtg gtgatgattg tgctgtcgcg
     6241 ccattcgttg gtggtggaag tgaagcgtca gttttgtcgc agcgaaaaaa tgaaaatgct
     6301 tttacgggcg gggtgaatgg ttgttttagg ctccggcaat aaaaacaaca acataacctc
     6361 acctggcacc aaatttatga ggaaaaaatg aaattactta ttctgggcaa ccacacttgc
     6421 ggcaatcgtg gcgacagcgc catcttgcgc ggcttacttg atgccatcaa catcctcaat
     6481 ccacatgccg aagtggacgt gatgagccgc tatccggtca gttcttcctg gctgctcaac
     6541 cgcccggtaa tgggcgatcc gctgttcctg caaatgaaac aacacaacag cgcggcgggc
     6601 gttgtcgggc gcgttaaaaa agtcctccgt cgccgctatc agcaccaggt attgctctca
     6661 cgcgtcaccg acactggcaa gctgcgtaat atcgccatcg cccagggatt caccgacttc
     6721 gtgcgcctgc tgtcaggtta cgacgccatt attcaggtcg gcggatcgtt ttttgtcgat
     6781 ctctatggcg ttccgcagtt tgaacatgcg ctttgcactt tcatggcgaa aaagccgctg
     6841 tttatgattg gtcacagcgt cggcccgttc caggatgagc aatttaacca actggcgaac
     6901 tacgtttttg gtcactgtga cgcgctgatc ctgcgcgaat cggtcagcct tgatttgatg
     6961 aaacgcagca atatcaccac cgcaaaagtg gaacatggcg tcgataccgc gtggctggtc
     7021 gatcaccaca cagaagactt caccgccagc tatgccgttc aacattggct ggacgttgcc
     7081 gcacaacaga aaacggtggc aattaccctg cgcgaactgg caccgttcga caaacgtctc
     7141 ggcaccactc aacaagcgta tgaaaaagcc tttgccgggg tggtcaatcg cattctcgac
     7201 gaaggctatc aggttattgc gctctccacc tgtacgggca tcgacagcta taacaaagac
     7261 gaccgcatgg tggcgctcaa cctgcgccag cacatcagcg atcctgcccg ttaccacgtg
     7321 gtgatggatg aactcaacga tctggaaatg ggcaaaattc tcggtgcctg tgaactcacc
     7381 gtcggtacgc gcctgcactc agccattatc tcaatgaact ttgctactcc ggcgattgcc
     7441 atcaactatg aacataaatc cgccgggatt atgcaacaac tggggctacc agagatggca
     7501 attgatatcc gtcatttact ggacggcagc ctgcaagcga tggttgcgga taccttaggc
     7561 cagcttccgg cgctgaacgc acgacttaac gaagctgtca gtcgtgagcg tcatacagga
     7621 atgcagatgg tgcaatctgt gcttgaacgc atcggggagg tgaaatgaag gtcggcttct
     7681 ttttactgaa atttccgctg tcgtcggaaa ccttcgtcct caatcaaatt accgcgttta
     7741 ttgatatggg ctttgaggtg gagattgtcg cgctgcaaaa aggcgacacc cagaacaccc
     7801 acgcggcatg gacgaaatac aaccttgctg ccagaacccg ctggttacag gacgaaccta
     7861 cgggcaaagt ggcgaaactg cgccaccgcg ccagccagac gttacgcggc attcatcgta
     7921 aaaatacctg gcaggcgctc aacctcaaac gctatggcgc cgagtcgcgg aacctgattt
     7981 tgtctgccat ttgtggtcag gtcgcaacac cgtttcgcgc cgatgtgttc atcgctcatt
     8041 ttggccctgc gggggtaacc gcagcaaaac tacgcgaact gggtgtcatt cgcggcaaaa
     8101 ttgccactat cttccacggt attgatatct ccagtcggga cgtgctcaac cactacactc
     8161 ccgaatatca acaactgttt cgccgtggcg acctgatgtt accgataagc gatttgtggg
     8221 ccggaaggct gcaaaaaatg ggctgtccga gggaaaaaat cgccgtatcg cgtatgggcg
     8281 tggacatgac gcgctttagc ccgcgtccgg tgaaagcgcc cgcaacgccg ctggaaatca
     8341 tctccgtcgc acgcttaacc gagaaaaaag gcctgcatgt ggcgatcgaa gcctgccggc
     8401 agttgaaaga gcagggcgtg gcatttcgct atcgcatcct cggcattggc ccgtgggaac
     8461 gacgcctgcg cacgctcatc gaacaatatc aactggaaga tgtggtagag atgccgggct
     8521 ttaaaccgag ccatgaagtg aaagcgatgc tcgacgacgc ggatgtcttc ctgttgccat
     8581 cgataacggg tgcggatggc gatatggaag gtattccggt ggcgctaatg gaagcgatgg
     8641 cggtcggcat tccggttgtt tcaactctgc atagcggaat accagaactg gtggaggccg
     8701 ataaatccgg ctggctggtg cctgagaacg atgctcgcgc actggcgcaa cgactggcgg
     8761 cgtttagcca actgaacacc gacgaactgg ctccggtcgt caaacgtgcg cgcgaaaaag
     8821 tcgaacacga ttttaaccag caggtgatta atcgagaact cgccagcttg ctgcaggctt
     8881 tatagaggag aacgtatgcc atttaaaaaa ctctcccgac gcaccttcct gacggcaagc
     8941 tccgcgcttg ccttcctcca tacccctttc gcccgtgcac ttcccgcccg acaaagcgtt
     9001 aacattaacg actacaaccc acacgactgg atcgcctcat ttaaacaagc cttcggcgaa
     9061 gggcaaacgg tcgtcgtgcc tgccggattg gtttgcgaca atatcaacac cggcatcttc
     9121 attcctgcag gtaaaacgtt acacatcctt ggaagcctgc gcggcaacgg cagagggcga
     9181 tttgtcttac aggacagcag ccaggtgaca ggggaggagg gtggcagtat gcataacatc
     9241 accctggatg tgcgtggctc tgactgcacc atcaaagggc tgacgatgag cggctttggt
     9301 ccggtaacgc agatttatat cggcggcaaa aacaaacgag taatgcgtaa cctctccatc
     9361 gataacctga ccgttagcca cgctaattac gccatcttac gccagggatt tcataaccag
     9421 attatcggtg ccaacatcac caactgtaaa tttagtgatt tacagggcga cgccattgaa
     9481 tggaacgtgg cgattaacga ccgtgatatt ttgatatctg accatgtcat cgagcgcatc
     9541 aactgtacca acggcaaaat caactggggt atcggcatag gccttgcggg aagtacctac
     9601 gataataact acccggaaga ccaggcagtg aaaaactttg tcgtggcgaa tatcacggga
     9661 tcggattgtc ggcagttgat ccatgttgaa aatggtaaac attttgttat tcgtaatatc
     9721 aaagcccgca atatcacgcc ggatttcagc aagaaagcag gcattgataa cgcgacagtt
     9781 gctatttacg gttgtgacaa tttcgtgatt gataatattg aaatgattaa tagcgctggg
     9841 atgttaatcg gctatggggt aattaaaggc aaatatctct caataccgca aaatttccga
     9901 gtgaataata ttcaaatgga taacacccat cttgcttata aattgcgcgg tatccaaatc
     9961 tctgccggga atgctgtctc ctttgtggcg ctgactaaca ttgagatgaa gcgtgcgtcg
    10021 ctggagttac acaacaaacc gcaacatctt tttatgcgaa atatcaaggt gatgcaggaa
    10081 tcctcagttg gacccgcatt gagcatgaac ttcgacatgc gcaaagacgt tcgcggcgtc
    10141 tttatggcga aaaaagaaac actgctgtca cttgcaaatg ttcatgcggt gaatgaaaaa
    10201 gggcaaagct ccgtcgatat cgacagagtt aatcaccaca ttgttaatgt ggaaaagatt
    10261 aactttagat tgccagaacg gagggagtag atttgcgacc attcataaac ttactggata
    10321 taatgttcta aaagattata atcctcacac taactgttaa agctggggct gggcagcatt
    10381 gactactcag ggaaggtact catctttatc ttgtgtttct tacgtaacaa ccagactaaa
    10441 gtgatttatg aacgataacg ttttgctcat aggagcttcc ggattcgtag gaacccgact
    10501 acttgaaacg gcaattgctg actttaatat caagaacctg gacaaacagc agagccactt
    10561 ttatccagaa atcacacaga ttggcgatgt tcgtgatcaa caggcactcg accaggcgtt
    10621 agccggtttt gacactgttg tactactggc agcggaacac cgcgatgacg tcagccctac
    10681 ttctctctat tatgatgtca acgttcaggg tacccgcaat gtgctggcgg ccatggaaaa
    10741 aaatggcgtt aaaaatatca tctttaccag ttccgttgct gtttatggtt tgaacaaaca
    10801 caaccctgac gaaaaccatc cacacgatcc tttcaaccac tacggcaaaa gtaagtggca
    10861 ggcagaggaa gtgctgcgtg aatggtataa caaagcacca acagaacgtt cattaactat
    10921 catccgtcct accgttatct tcggtgaacg caaccgcggt aacgtctata acttgctgaa
    10981 acagatcgct ggcggcaagt ttatgatggt gggcgcaggg actaactata agtccatggc
    11041 ttatgttgga aacattgttg agtttatcaa gtacaaactg aagaatgttg ccgcaggtta
    11101 tgaggtttat aactacgttg ataagccaga cctgaacatg aaccagttgg ttgctgaagt
    11161 tgaacaaagc ctgaacaaaa agatcccttc tatgcacttg ccttacccac taggaatgct
    11221 gggtggatat tgctttgata tcctgagcaa aattacgggc aaaaaatacg ctgtcagctc
    11281 agtgcgcgtg aaaaaattct gcgcaacaac acagtttgac gcaacgaaag tgcattcttc
    11341 aggttttgtg gcaccgtata cgctgtcgca aggtctggat cgaacactgc agtatgaatt
    11401 cgttcatgcc aaaaaagacg acataacgtt tgtttctgag taatgcagac gagtcagaga
    11461 aataaggacc aggttggact acgattattc ggaaagaggt actgattaaa ttatcagtat
    11521 gcggaattcc gagtaatccg agtaacccag atgctgatgg tgtgtagtgt tattctaagg
    11581 tttaagcaaa gttatgcgga actaagcttt ggtatatagc cataacgttt ggctaaccta
    11641 tggcaataat ttacgcacta tcagccactt agaggtagga aaagaatgac gaatttaaaa
    11701 gcagttatac cggtagcagg tcttgggatg catatgttgc ctgccactaa ggcgattccc
    11761 aaagagatgc taccgatcgt cgacaagcca atgattcagt acattgttga cgagattgtg
    11821 gctgcaggga tcaaagaaat cctcctggta actcacgcgt ccaagaacgc agtcgaaaac
    11881 cacttcgaca cctcttatga attagaatct ctccttgaac agcgcgtgaa gcgtcaactg
    11941 ctggcggaag tacagtctat ctgtccgccg ggcgtgacca ttatgaacgt gcgtcagggc
    12001 gaacctttag gtttaggcca ctccattttg tgtgcacgac ccgccattgg tgacaaccca
    12061 tttgtcgtgg tattgccgga cgttgtgatc gacgacgcca gtgccgaccc gctgcgctac
    12121 aaccttgctg ccatgattgc acgtttcaac gaaacgggcc gcagccaggt gctggcaaaa
    12181 cgtatgccgg gtgacctctc tgaatactcc gtcattcaga ctaaagagcc gctggatcgt
    12241 gaaggtaaag tcagccgtat tgttgaattc atagaaaagc cggatcagcc gcagacgctg
    12301 gactcagaca tcatggccgt tggtcgatat gtgctttctg ccgatatttg gccggaactt
    12361 gaacgcactc agcctggtgc atgggggcgt attcagctga ctgatgccat tgccgaactg
    12421 gcgaaaaaac agtccgttga tgccatgctg atgactggtg acagctatga ctgcggtaaa
    12481 aaaatgggtt atatgcaggc gtttgtgaag tatggactgc gcaacctcaa agaaggggcg
    12541 aagttccgca aaggcattga gaaactgtta agcgaataat gaaaatctga ccgaatgtga
    12601 cggttggcaa ggaaactata acagcagtga agattcgtgg cgaaagtaat ttgttgcgaa
    12661 tattcctgcc gttattttgt ataaacaatc agaataacaa atagttagca ataggctttt
    12721 cgtcaaagtt ttcctggatt ttccttgttt ccagaacgga ttggtaagac aattagcgtt
    12781 tgagtttcga gggagatgca gcaatgtctc tcagtaacag tacgcaatat caatgagttc
    12841 agtgcactgg tagctgttaa gccaggggcg gtagcgtgga ttgatggaat gaaaaataag
    12901 tgcccacgaa ttgcagtaat aatgtcggtt tatattaatg ataaatttaa tgagtttaaa
    12961 gaggcttttg acagtattat taatcaaaca tactctaact ttgttttctt tatttatgcc
    13021 gatggcaaga taaatccaga tattgaaagt tttttgcaaa attataattc gcaacatgtg
    13081 attttcatta gaagtgaaaa aaataaggga ttagcttatg ctttgaataa attaatatca
    13141 gtggcgctag ttaataatga atttgatttt attgccagaa tggatgctga tgatataagt
    13201 ttagaagata gatttcaacg acaaatagat ttttttatgg ctaaccctga gttagatgtt
    13261 gttggtgggt tgtgtcaaga gtttggtggt gagtcagcaa gagaaaaatg caagatctat
    13321 tttacagatg aagccattaa aaacaatgcc tttaaaaaat gtcctttcgt tcatccaacc
    13381 gtgatgttta aatcgtctgt atttaataac gggatgcgat accctgaaaa tcagccactg
    13441 acagaagata tgtgcttatg gctcgagtta atatccgcga ataaaaagtt tgcaaatatt
    13501 gactccgttc ttctaaagta cagaattaat gagcagacgc taaaaagaag aagaggtttg
    13561 aataaggctg taaccgaatt atccacaaga ttgtattacg ccaagaaact taaaatgctt
    13621 agcgtacgga atttgttata tatattaatc cattttggtg tcagagtcct tccttcaaat
    13681 atatttaaac ttgtacataa gttaaattaa aatgaataaa actgtttata catatgatgt
    13741 atgggatact attttaaaac gtcattgttt accatcatat acgttagagg tttcgttgta
    13801 ctggctactt ttgtatcttg gaaaacctga gaaacataag gatgtgtata aaaaagtaaa
    13861 agcactggag agtgattttt ttaatcgaaa agaagaatat tttattgaag agcttattgt
    13921 tgggatatta attgaaaata atttaatgaa tggattttgc aataactctg tgactgctgc
    13981 ttgggaacaa atatattatt tcgtagagag aaattgtaca tataaaaata aagaggttat
    14041 agagttaatt gggagtgact atggggacaa attatttatt tctgattttt atactgactc
    14101 cagtttcata aagaaattgc tacaccacca ccaagtatcg gttttagatg gtgtgacgtc
    14161 agctgagtat ggcgtcagta agcatatggg ttcattgtat gaaaaaatag aactattgaa
    14221 gcaaaggaaa tggatccata ctggagataa tgaattagct gatataaaaa tggcaaagcg
    14281 tgcaggtgcc caagttagac ttataaaatc caaaaagaag aaaataaata agttaaaaat
    14341 aaaaaatgac tatgagcggt tagcatttat tattttgtcc tttagcctgt ttatattaaa
    14401 aacttccaga aagaaaaagt gcaataagat ctactttttt acaagggagg gggttttttt
    14461 caaaaaaaac tttgatcttc tactaaacca aatacctaac atattcccat ctataactac
    14521 ggaaatattg ccaatcagtc gagtagccag cttagcattg aagtttaatg agtctgatga
    14581 atttttagga tttaatgatg cattaaccca gtatggctat ggagtgtcta cgtttcttag
    14641 tttctttcat cttgatgatt gttactccga gctcacaaca aagtatcatg atgtaaatga
    14701 tttattatct cacagaaatg atcctttggt aaaacaacta ataaaaagta tagaggataa
    14761 aaaaaaacga acagaaaatt ttcttacatc cattgagttc gattctgagc caagtatcat
    14821 cgtggatatt ggttggcggg gtagcatcca agataattta atgtcgcgga aaaataatta
    14881 tattcaacat ggatgttatc tcggattgtt tgatttttac ccaggccagt caggtcagaa
    14941 taaatcatct gtaatttttg ataacaacag atttagacaa aaatggacca tgaaaggtgt
    15001 agcttttatg gagacggttt tcaatgcttt ggacggaagt gttgtcgatt atgtaaacaa
    15061 taaaccgaaa agaaaagaaa attacgctga gatagaaaat tcaaaacatt taatagttat
    15121 gcaagatgct atcattgagg aatttatgaa tctatctatt atgttaaaag agggaaagtt
    15181 aaatatcaat gatattgaaa gattagctat tgaaagttac aaaaaaatag taactaaccc
    15241 atctaaagaa atagctgatt tttatgttgg cagtatccaa aatgaaagct tcggtttgaa
    15301 tgattttatc gacaaagaat ttaacattag cgcagtagat gttttccgaa gttttttttc
    15361 taaaagtaaa agaaatgaaa taagaataaa attgcataga aatggatggc gtgagtcgat
    15421 acttaaatcc tctaaagtat cgctgagcgc aaaattatgt tcacttttaa ttagataaaa
    15481 tatgaatatt tcagtaataa tttgttgcta taactcagaa aatagaatcg taaacaccct
    15541 ttttcatctt tcacaatgtg tatctctatc caaggggtta attgacgaga ttattcttgt
    15601 tgacaataac tcatctgatg aaactgttaa aacagctgag gatttttggc gtaatacggg
    15661 ggaggagcaa atctttaaaa ttgtttttga atcgattccg ggccttgttc atgctagacg
    15721 gcgagggctg gacgagtcca aaaatgagat tgttgttttt tgtgatgatg ataattggct
    15781 ggtaccatct tattttacaa aactagagaa atattttgaa aacaaacaag taggcgctgt
    15841 cgggggacgc ggtattccta ttgctaatac cgttattcct gagtggtttt ttgagaatct
    15901 ggatgcatat gcatgttctg cacaaacttc taacatgttg tatggtgcta gcttggctat
    15961 tcggtctagt atcttaaaaa aattttatac ggatgcttta tcagtaaagc taaagggtag
    16021 aacaaaagac agtctggatt ctggggaaga taacttattg tgtaattttg tcaaaaaaca
    16081 aggatacact ctgatacctg acaatacacc attttgccat ttcatggaaa gcaagcgatt
    16141 ggatgtcgat tatctatata aactggctga atcatttgga aaagcaacgg catcaatgaa
    16201 aaacaattcc ttcaccaccg attatgtata tttgacagca aaaatgttat tagctatatc
    16261 taggatcttc tctaaaaaaa ataaaaagat atatttaaaa cgtgagattt cctttctcag
    16321 aggttatttg aggtttagaa aggaatcgaa gtaatgagag ggggaatgct tgtaattgcg
    16381 tggtattcta tttttctgtt gatttcagct acgggcatga ccggtctaaa taagctgagt
    16441 actgaagcta ttctgtgcta tattgcattt ttattctgtt ttttctttgg catgatgtta
    16501 ggtggtgttt cgctcccaaa cggaatgaaa atgttgcaac aggagcaaaa gataaagaaa
    16561 aggcatgctt ctgccatagt tctaatttat tgctttttta ttttttattc tttaattgct
    16621 cttttaaata tatattattt caataaggtt gatctatcat cttatagaca agcatttttt
    16681 gatggttcaa gagataatgt taaatacttc tatggcgtgt ttggctttta cttctattat
    16741 ttacttagcc tgttagtatt cgccttagtt ccttatacta ttataacgaa cagcaaaaag
    16801 actatttcat tatgtttctt tgccttgctg ctatacgata ttattttcct ttcaagaact
    16861 ggtgtttatt actttatatt atcttatata gtatcgagta ttcttctcaa gaaaggaatc
    16921 aagaaaatta taattgtagg gatggttgcc ttattattct cattgataat gagttacaca
    16981 agggatatga agagtgacat cattggtaca ttatttagct caattataaa ttatcatata
    17041 gctccgtttg tcttgtttga tgaaaatata atatcaaatc atttgattaa atataatggg
    17101 gctggcttgg catcgttggg gatatataat atcatttttt atcccattga ttcgtctatc
    17161 atgtcatcaa taaacgattt tagagcacag ttgaatgaat tctatgattt gggagtaaat
    17221 cgttatttgc catataatgc ctattatact tctttgggtg gggtatatat agatagcggc
    17281 ctgattggta gcatgatggt tagttttatg actggtttta ttattgtcgg aatcgagaag
    17341 agtgctttta aatcaactaa gttcttagtc tcttctgtgt tttttactac attatgtatg
    17401 gaatctatat tttccccaat aacactaaat atttttgcct ttacaataat tgtctactta
    17461 gttgttttga tggtccttaa atatgaaaat agcgatgata ataccatctt taaaaaatac
    17521 cgcgccaaat aatatagccc tttcaatatg tgaaggggca aggaagcatg gtgtcaatat
    17581 agatatttat tatcttgatg aaattgtaga gttggatata gacgagtcag aactgaatat
    17641 aaataggcta aatgatagaa ccattttaaa cagttatgat attgttcact ctcatggctt
    17701 aagacccgat gttattaatt gtaagaccag caaaaatgtc attaatgtaa gtacgcttca
    17761 tagttttatt ttagatgatc ttaagaataa atatggaata aaaggatatc tgatcgcttt
    17821 tgcttggctt aaattattaa agcgatttga ttcatgtatt gcaattagtg aggcgactaa
    17881 acggttttat gttaaacatg gtcttaaatg ccaccatata tataatggaa tcgactttcc
    17941 tgacatcgaa ttatatcaag ataactcaag gctaaataac ggcaaaataa atatagtatc
    18001 gatcgcgcat ctggaaaaaa taaaaggctt agagcaactc ctatatctag ccgcggagcg
    18061 agaagagttc catgttcata taattggtga aggaacttac cgtgggaaat taacaaatat
    18121 tattgataag tttgatttat ctcagcgtgt aactttgcat ggatatatat caaatgcgag
    18181 ttctatgttg gggaaatttg atgtttatgt gcaaccatct aaaagtgagg ggtttggcat
    18241 tgcagtcatt gaagctcttg taaataaaat cccaacagtt tgctcagaca ttgaagtgtt
    18301 cagagagtta tttggctcag gagaagttga atttttcaag ttagacgata ttaattcact
    18361 ttacgatgca atttcaagtg cattaaccaa gacagatatg tctcgagatg caagtgccac
    18421 agcgataagc caaaaatttt cttcagaagt gatgtcattg aactatttaa gttggtataa
    18481 aaaattatat gaagaaagaa atttataaat atggcacaat aatattatgc tatggaatta
    18541 actttatact acctattctt ttctttaaag aaataagttt aatattatct ccaatagtat
    18601 tatcatcatt tttctttgtt ttggcattgc taatgtattg tcaattcatt gttgaatacg
    18661 ggttgcaaat atcattatta agaaagttaa atgaaaataa gagtgattta agtcggcttt
    18721 tatctactat tcttggacta aaagtaatac tattcgtcct atgcgcagta ttcatttatt
    18781 gtattttgct atataataat gaagttatat tttttatact tgttttcatt ctgctaggaa
    18841 acgtattctc atgccagttc ctctatcaag tagttgatca gctccatttt ttttatgtat
    18901 taaattcgct tgttaagcta attttcatac ccctcatttt tatcaatgat aattatatct
    18961 atctgatgat atgttatagt tgtttcaata tcctacctaa cttagctgca cttgcttatt
    19021 ttttatataa gaataaagtt aaggttgtaa aagttcctgc ttgtgaaatt tataatttat
    19081 ctaaggaatg ttttagctat tttatctcga atatttcgat aacattatac actaattttt
    19141 atcagatctt attaggcatt atatcgcccg cttatctggc cgcgtatgca ttaagtgaca
    19201 aaattattag ggggatcgtg tcagggaatt atgtgtttat acagatagta caagtacaat
    19261 acttaaatga tgaatcaaag ttcatcaatg gtacatggaa aacgattgtt tcattattat
    19321 tactgggggg gatggagttt ttatttattt tctttggagc ggattttttc tctcgttatt
    19381 tattccctac aatatcttta ctgccaattt ttctaaaact aatgtcattg ctaattgttg
    19441 ttatcttgtt gagtaacttt tttgcaatgg tttatcttcc gtgttcaggc aatactttat
    19501 tcctctcacg tgttctgatt tttgtttcta ttgtgtccgt agtagttgcc ccgatactaa
    19561 tatggctcta tgcaggttat ggtgcagtca tttccgctat attttcagag ttattagttc
    19621 tgattttatg ctatggttta tatttgaaat taaaacacaa actctaacta ttaacgtaga
    19681 ataattaaaa tgaatttatt gattgttttt tgcttttata tttcggttct gcgtagtaat
    19741 aaaataagtg tatttaaatt aatcactatc acaactgtct tgattgtata tgtattctgg
    19801 ctaatagccg acagttttac tggcgttggg ataaatgatt cagtttattt ccatatgtta
    19861 atgccgcatg agggggcatc ggttgaaaat ttaacatatt atggatttct gattgtaagc
    19921 cttgttattt caattttctt gattgcttat gtttcttgga aaaagaagca taatagagga
    19981 ttaagtaaaa attactcttt ttacgtactc ttcgctattg tattagttac tccatttatg
    20041 cgtaatttcc tggggtcaat taacaattta atttccgaac agttttctac tgaagatata
    20101 agtaaagagt atgtaatcaa ta
//
LOCUS       DAMZFT010000087        20085 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.087, whole genome shotgun sequence.
ACCESSION   DAMZFT010000087 DAMZFT010000000
VERSION     DAMZFT010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20085)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20085)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20085
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.087"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(<1..1402)
                     /locus_tag="PN977_003286"
     CDS             complement(<1..1402)
                     /locus_tag="PN977_003286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HDI6043291.1"
                     /translation="MFSRFFVRRPVFAWVIAILIMLAGILAIRTLPVAQYPDVAPPTI
                     KISATYTGASAETLENSVTQVIEQQLTGLDNLLYFSSTSSSDGSVSINVTFEQGTDPD
                     TAQVQVQNKIQQAESRLPSEVQQTGVTVEKSQSNFLLIAAVYDTTDKASSSDIADWLV
                     SNVQDPLARVEGVGSLQVFGAEYAMRIWLDPAKLASYSLMPSDVQSAIEAQNVQVTAG
                     KIGALPSPNTQQLTATVRAQSRLQTVDQFKNIIVKSQSDGAVVRIKDVARVEMGSEDY
                     TAIGKLNGHPSAGVAVMLSPGANALNTATLVKDKIAEFQRNMPQGYDIAYPKDSTEFI
                     KISVEDVIQTLFEAIVLVVCVMYLFLQNLRATLIPALAVPVVLLGTFGVLALFGYSIN
                     TLTLFAMVLAIGLLVDDAIVVVENVERIMRDEGLPAREATEKSMGEISGALVAIALVL
                     SAVFLPMAFFGGSTGVI"
     gene            complement(1402..2523)
                     /locus_tag="PN977_003287"
     CDS             complement(1402..2523)
                     /locus_tag="PN977_003287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HDI6043292.1"
                     /translation="MKYIATSVVAMLLLSGCDNTQSNNSSPSETEVGVVTLKSQPVSV
                     VSELTGRTSAALSAEVRPQVGGIIQKRLFKEGDLVKAGQPLYQIDAASYQAAWNEARA
                     ALQQAQALVKADCQKAQRYARLVKENGVSQQDADDAQSTCAQDKASVEAKKAALETAR
                     INLDWTTVTAPISGRIGISSVTPGALVTASQDTALTTIRGLDTMYVDLTRSSVDLLRL
                     RKQSLATNSDTMSVSLILEDGTTYSEKGRLELTEVAVDESTGSVTLRAIFPNPQQQLL
                     PGMFVRARVDEGVMEDAILAPQQGVTRDAKGNATALVVNKDNKVEQRALETGETYGDK
                     WLVLNGLHNGDRLIVEGSAKVTSGQTVKAVEVQANGGNA"
     gene            complement(2672..3238)
                     /locus_tag="PN977_003288"
     CDS             complement(2672..3238)
                     /locus_tag="PN977_003288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDI6043293.1"
                     /translation="MTSKLEIRHKQRQDEIINAARRCFRRCGFHAASMSQIASEAQLS
                     VGQIYRYFANKDAIIEEMVRRIIDFRIAQMDIDARTDHFPEVLALRKSLNEDDDALML
                     EVAAEATRNPRVMAMLEEADARMFANGCAHMKRMHPHLSDEHIRCCVEVFATMMEGTV
                     YRRLTPQKSDPQHLQEIYQDIVSMLINK"
     gene            3716..4504
                     /gene="fabI"
                     /locus_tag="PN977_003289"
     CDS             3716..4504
                     /gene="fabI"
                     /locus_tag="PN977_003289"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HDI6043294.1"
                     /translation="MGFLSGKRILVTGVASKLSIAYGIAQAMHREGAELAFTYQNDKL
                     KGRVEEFAAQLGSDIVLQCDVAEDASIDTMFAELGKVWPKFDGFVHSIGFAPGDQLDG
                     DYVNAVTREGFKIAHDISSYSFVAMAKACRSMLNPGSALLTLSYLGAERAIPNYNVMG
                     LAKASLEANVRYMANAMGPEGVRVNAISAGPIRTLAASGIKDFRKMLAHCEAVTPIRR
                     TVTIEDVGNSAAFLCSDLSAGISGEVVHVDGGFSIAAMNELELK"
     gene            4649..5776
                     /locus_tag="PN977_003290"
     CDS             4649..5776
                     /locus_tag="PN977_003290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707196.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CMD domain-containing protein"
                     /protein_id="HDI6043295.1"
                     /translation="MEQRHITGKSHWYHETQSSTAEYDVLPLVPEAAKVSDPFLLDVI
                     LDEETLAPFLSWLVPARVLVVELFPDQLTVTRSQTFTAYERLSTALTVAQVCGVQRLC
                     NYYSARLTPLPGPDSSRESNHRLAQITQYARQLASSPSIIDNRSRQHLNDVGLTACDC
                     VIINQIIGFIGFQARTIATFQAYLGHPVRWLPGLEIQNYADASLFADESLRWRSSYEV
                     EKLPEEHTKSSTAELCQLANTLSLHPISLSLLEKLLNSTRVNTQPDNQLAALLCARIN
                     GSPACFAACMNSSNEYKKISLLLRKGENEINRWADRHSVERATVQAIQWLTRAPDRFS
                     AAQFSPLLEHEKSSTQIINLLVWSGLCGWINRLKIALGETY"
     gene            5844..7778
                     /gene="rnb"
                     /locus_tag="PN977_003291"
     CDS             5844..7778
                     /gene="rnb"
                     /locus_tag="PN977_003291"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exoribonuclease II"
                     /protein_id="HDI6043296.1"
                     /translation="MFQDNPLLAQLKQQLHSQTPRAEGVVKATEKGFGFLEVDAQKSY
                     FIPPPQMKKVMHGDRIIAVIHSEKERESAEPEELVEPFLTRFVGKVQGKNDRLAIVPD
                     HPLLKDAIPCRAARGLNHEFKEGDWAVAEMRRHPLKGDRSFYAELTQYITFGDDHFVP
                     WWVTLARHNLEKEAPDGVATEMLDEGLVREDLTALDFVTIDSASTEDMDDALFAKALP
                     DDKLQLIVAIADPTAWIAEGSKLDKAAKIRAFTNYLPGFNIPMLPRELSDDLCSLRAN
                     EVRPVLACRMTLSADGTIEDNIEFFAATIESKAKLVYDQVSDWLENTGDWQPESEAIA
                     EQVRLLAQICQRRGEWRHNHALVFKDRPDYRFILGEKGEVLDIVAEPRRIANRIVEEA
                     MIAANICAARVLRDKLGFGIYNVHMGFDPANADALAALLKTHGLHVDAEEVLTLDGFC
                     KLRRELDAQPTGFLDSRIRRFQSFAEISTEPGPHFGLGLEAYATWTSPIRKYGDMINH
                     RLLKAVIKGETATRPQDEITVQMAERRRLNRMAERDVGDWLYARFLKDKAGTDTRFAA
                     EIVDISRGGMRVRLVDNGAIAFIPAPFLHAVRDELVCSQENGTVQIKGETAYKVTDVI
                     DVTIAEVRMETRSIIARPVA"
     gene            complement(7855..7959)
                     /locus_tag="PN977_003292"
     CDS             complement(7855..7959)
                     /locus_tag="PN977_003292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6043297.1"
                     /translation="MTYMKLTAAKRIVFFIYLFVIQFWKNASFQTKVS"
     gene            8013..9998
                     /gene="pdeR"
                     /locus_tag="PN977_003293"
     CDS             8013..9998
                     /gene="pdeR"
                     /locus_tag="PN977_003293"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
                     /protein_id="HDI6043298.1"
                     /translation="MKTVRESTTLYNFLGSHNPYWRLTESSDVLRFSTTETTEPERTL
                     QLSAEQAARIREMTVITSSLMMSLTVDESDLSVHLVGRKINKREWAGNASAWHDTPAV
                     ARDLSHGLSFAEQVVSEAHSAIVILDSRGNIQRFNRLCEDYTGLKEHDVIGQSVFKLF
                     MSRREAAASRRNNRVFFRSGNAYEVELWIPTRKGQRLFLFRNKFVHSGSGKNEIFLIC
                     SGTDITEERRAQERLRILANTDSITGLPNRNAMQELIDHAINQADNNKVGVVYLDLDN
                     FKKVNDAYGHLFGDQLLRDVSLAILSCLEHDQVLARPGGDEFLVLASNTSQSALEAMA
                     SRILTRLRLPFRIGLIEVYTSCSVGISLSPEHGSDSAAIIRHADTAMYTAKEGGRGQF
                     CVFTPEMNQRVFEYLWLDTNLRKALENDQLVIHYQPKITWRGEVRSLEALVRWQSPER
                     GLIPPLDFISYAEESGLIVPLGRWVILDVVRQVAKWRDKGINLRVAVNISARQLADQT
                     IFTALKQVLQELNFEYCPIDVELTESCLIENDELALSVIQQFSQLGAQVHLDDFGTGY
                     SSLSQLARFPIDAIKLDQVFVRDIHKQPVSQSLVRAIVAVAQALNLQVIAEGVESKKE
                     DAFLTKNGINERQGFLFAKPMPAVAFERWYKRYLKRA"
     gene            10146..10319
                     /locus_tag="PN977_003294"
     CDS             10146..10319
                     /locus_tag="PN977_003294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6043299.1"
                     /translation="MSEFDAQRVAERIDIVLDILVAGDYHSAIHNLEILKAELLRQVA
                     ESTPDIPKAPWEI"
     gene            10409..11158
                     /locus_tag="PN977_003295"
     CDS             10409..11158
                     /locus_tag="PN977_003295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HDI6043300.1"
                     /translation="MNSRQQTILQMVIDQGQVSVTDLAKATGVSEVTIRQDLNTLEKL
                     SYLRRAHGFAVSLDSDDVETRMMSNYTLKRELAEFAASLVQPGETIFIENGSSNALLA
                     RTLGEQKKNVTIITVSSYIAHLLKDAPCEVILLGGVYQKKSESMVGPLTRQCIQQVHF
                     SKAFIGIDGWQPETGFTGRDMMRTDVVNAVLEKECEAIVLTDSSKFGAVHSYSIGPVE
                     RFNRVITDSKIRASDLMHLEHSKLTVHVVDI"
     gene            11427..11645
                     /gene="osmB"
                     /locus_tag="PN977_003296"
     CDS             11427..11645
                     /gene="osmB"
                     /locus_tag="PN977_003296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmB"
                     /protein_id="HDI6043301.1"
                     /translation="MFVTSKKMTAAVLAITLAMSLSACSNWSKRDRNTAIGAGAGALG
                     GAVLTDGSTLGTLGGAAVGGVIGHQVGK"
     gene            complement(11771..12097)
                     /gene="yciH"
                     /locus_tag="PN977_003297"
     CDS             complement(11771..12097)
                     /gene="yciH"
                     /locus_tag="PN977_003297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response translation initiation inhibitor
                     YciH"
                     /protein_id="HDI6043302.1"
                     /translation="MSDSNSRLVYSTETGRIDEPKAAPVRPKGDGVVRIQRQTSGRKG
                     KGVCLITGVDLDDAELTKLAAELKKKCGCGGAVKDGIIEIQGDKRDLLKSLLEAKGMK
                     VKLAGG"
     gene            complement(12097..12834)
                     /gene="pyrF"
                     /locus_tag="PN977_003298"
     CDS             complement(12097..12834)
                     /gene="pyrF"
                     /locus_tag="PN977_003298"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HDI6043303.1"
                     /translation="MTLTASSSSRAVTNSPVVVALDYHNRDAALAFVDKIDPRDCRLK
                     VGKEMFTLFGPQFVRELQQRGFDIFLDLKFHDIPNTAAHAVAAAADLGVWMVNVHASG
                     GARMMTAAREALVPFGKDAPLLIAVTVLTSMEASDLADLGVTLSPADYAERLAALTQK
                     CGLDGVVCSAQEAVRFKQVFGQEFKLVTPGIRPQGSEAGDQRRIMTPEQALAAGVDYM
                     VIGRPVTQSVDPAQTLKAINASLQRSA"
     gene            complement(13026..14195)
                     /gene="lapB"
                     /locus_tag="PN977_003299"
     CDS             complement(13026..14195)
                     /gene="lapB"
                     /locus_tag="PN977_003299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapB"
                     /protein_id="HDI6043304.1"
                     /translation="MLELLFLLLPVAAAYGWYMGRRSAQQNKQDEANRLSRDYVAGVN
                     FLLSNQQDKAVDLFLDMLKEDTGTVEAHLTLGNLFRSRGEVDRAIRIHQTLMESASLT
                     YEQRLLAIQQLGRDYMAAGLYDRAEDMFNQLTDETDFRIGALQQLLQIYQATSEWQKA
                     IDVAERLVKLGKDKQRVEIAHFYCELALQHMASDDLDRAMTLLKKGAAADKNSARVSI
                     MMGRVFMAKGEYAKAVESLQRVISQDRELVSETLEMLQTCYQQLGKTAEWAEFLQRAV
                     EENTGADAELMLADIIEARDGSEAAQVYITRQLQRHPTMRVFHKLMDYHLNEAEEGRA
                     KESLMVLRDMVGEKVRSKPRYRCQKCGFTAYTLYWHCPSCRAWSTIKPIRGLDGL"
     gene            complement(14202..14510)
                     /gene="lapA"
                     /locus_tag="PN977_003300"
     CDS             complement(14202..14510)
                     /gene="lapA"
                     /locus_tag="PN977_003300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapA"
                     /protein_id="HDI6043305.1"
                     /translation="MKYLLIFLLVLAIFVISVTLGAQNDQQVTFNYLLAQGEYRISTL
                     LAVLFAAGFAIGWLICGLFWLRVRVSLARAERKIKRLENQLSPATDVAVAPHSSAAKE
                     "
     gene            complement(14659..15423)
                     /gene="pgpB"
                     /locus_tag="PN977_003301"
     CDS             complement(14659..15423)
                     /gene="pgpB"
                     /locus_tag="PN977_003301"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase B"
                     /protein_id="HDI6043306.1"
                     /translation="MRSIARRTAVGTALLLVMPVAVWISGWRWQPGEQSWLLKAAFWV
                     TETVTQPWGVITHLILFGWFLWCLRFRIKAAFVLFAILAAAILVGQGVKSWIKDKVQE
                     PRPFVIWLEKTHHIPVDEFYTLKRAERGNLVKEQLAEEKNIPQYLRSHWQKETGFAFP
                     SGHTMFAASWALLAVGLLWPRRRTLTIAILLVWATGVMGSRLLLGMHWPRDLVVATLI
                     SWALVAVATWLAQRICGPLTPPAEENREIAQREQES"
     gene            15593..16183
                     /gene="ribA"
                     /locus_tag="PN977_003302"
     CDS             15593..16183
                     /gene="ribA"
                     /locus_tag="PN977_003302"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HDI6043307.1"
                     /translation="MQLKRVAEAKLPTPWGDFLMVGFEELATGHDHVALVYGDISGHT
                     PVLARVHSECLTGDALFSLRCDCGFQLEAALTQIAEEGRGILLYHRQEGRNIGLLNKI
                     RAYALQDQGYDTVEANHQLGFAADERDFTLCADMFKLLGVNEVRLLTNNPKKVEILTE
                     AGINIVERVPLIVGRNPNNEHYLDTKAEKMGHLLNK"
     gene            complement(16247..18922)
                     /gene="acnA"
                     /locus_tag="PN977_003303"
     CDS             complement(16247..18922)
                     /gene="acnA"
                     /locus_tag="PN977_003303"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HDI6043308.1"
                     /translation="MSSTLREASKDTLQAKDKTYHYYSLPLAAKSLGDITRLPNSLKV
                     LLENLLRWQDGKSVTEEDIHALAGWLKNAHADREIAYRPARVLMQDFTGVPAVVDLAA
                     MREAVKRLGGDTAKVNPLSPVDLVIDHSVTVDRFGDDEAFEENVRLEMERNHERYVFL
                     KWGKQAFSRFSVVPPGTGICHQVNLEYLGKAVWSELQDGEWIAYPDTLVGTDSHTTMI
                     NGLGVLGWGVGGIEAEAAMLGQPVSMLIPDVVGFKLTGKLREGITATDLVLTVTQMLR
                     KHGVVGKFVEFYGDGLDSLPLADRATIANMSPEYGATCGFFPIDAVTLDYMRLSGRSE
                     DQVELVEKYAKAQGMWRNPGDEPIFTSTLELDMNDVEASLAGPKRPQDRVALPDVPKA
                     FAASNELEVNATHKDRQPVDYVMNGHQYQLPDGAVVIAAITSCTNTSNPSVLMAAGLL
                     AKKAVTLGLKRQPWVKASLAPGSKVVSDYLAKAKLTPYLDELGFNLVGYGCTTCIGNS
                     GPLPDPIETAIKKGDLTVGAVLSGNRNFEGRIHPLVKTNWLASPPLVVAYALAGNMNI
                     NLASEPIGHDRKGDPVYLKDIWPSAQEIARAVEQVSTEMFRKEYAEVFEGTAEWKEIN
                     VARSDTYGWQEDSTYIRLSPFFDEMQATPAPVEDIHGARILAMLGDSVTTDHISPAGS
                     IKPDSPAGRYLQGRGVERKDFNSYGSRRGNHEVMMRGTFANIRIRNEMVPGVEGGMTR
                     HLPDSEVISIYDAAMRYKQEQTPLAVIAGKEYGSGSSRDWAAKGPRLLGIRVVIAESF
                     ERIHRSNLIGMGILPLEFPQGVTRKTLGLTGEEKIDIGDLQNLQPGATVPVTLTRAHG
                     SQEVVPCRCRIDTATELTYYQNDGILHYVIRNMLK"
     gene            complement(19072..19152)
                     /locus_tag="PN977_003304"
     CDS             complement(19072..19152)
                     /locus_tag="PN977_003304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014832221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6043309.1"
                     /translation="MKYWSWMGAFSLSMLFWAELLWIITH"
     gene            complement(19242..19409)
                     /locus_tag="PN977_003305"
     CDS             complement(19242..19409)
                     /locus_tag="PN977_003305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_304581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6043310.1"
                     /translation="MVGQEQLESSPLCQHSDNEPEAKRECSVVIPDDWQLTSQQQAFI
                     ELFAEDDQPKQ"
     gene            complement(19412..19576)
                     /locus_tag="PN977_003306"
     CDS             complement(19412..19576)
                     /locus_tag="PN977_003306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmiA family putative membrane protein"
                     /protein_id="HDI6043311.1"
                     /translation="MLISDGDYMRLAMPSGNQEPRRNPELKRKAWLAVFLGSALSWVV
                     VALLIWKVWG"
     gene            complement(19871..>20085)
                     /gene="cysB"
                     /locus_tag="PN977_003307"
     CDS             complement(19871..>20085)
                     /gene="cysB"
                     /locus_tag="PN977_003307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012017585.1"
                     /note="LysR-type transcriptional regulator; contains
                     helix-turn-helix (HTH) motif; in Escherichia coli this
                     protein regulates cysteine biosynthesis by controlling
                     expression of the cys regulon; autoregulates expression;
                     crystal structure of Klebsiella aerogenes showed tetramer
                     formation; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HTH-type transcriptional regulator CysB"
                     /protein_id="HDI6043312.1"
                     /translation="DLVRVDAHDIFSHSTTKIGFRRSTFLRSYMYDFIQRFAPHLTRD
                     VVDAAVALRSNEEIEAMFKDIKLPEK"
BASE COUNT         5075 a         5185 c         4763 g         5062 t
ORIGIN      
        1 aaattacgcc cgtagatccc ccaaagaagg ccatcggcag gaatacggct gacaacacca
       61 gcgcaatggc aaccagtgcg ccagaaatct cgcccattga tttttccgtg gcttcacgcg
      121 cgggcaaccc ttcgtcacgc ataatgcgtt cgacgttctc taccaccaca atggcatcgt
      181 ccaccagcaa gccgatcgcc agcaccattg caaacaacgt cagggtatta atcgaatagc
      241 caaacaacgc aagaacgccg aatgtgccca gcaaaacgac ggggacagct aacgccggaa
      301 tcagtgtggc acgcaggttt tgcaggaata aatacatcac gcaaaccacc aatacgatgg
      361 cttcaaacag cgtctgaatt acgtcctcta cggagatttt aataaattca gtgctgtctt
      421 tcgggtatgc gatgtcgtat ccctgcggca tgttccgctg gaattcggca atcttatcct
      481 tgaccagcgt cgccgtattc agcgcattcg caccgggcga aagcattaca gcaaccccgg
      541 cagacgggtg accgttaagt ttgccgatag cggtataatc ttcactgccc atctcaacgc
      601 gagccacatc ttttatacga acaactgcgc cgtctgactg gcttttcacg ataatatttt
      661 tgaactgatc caccgtctgc aagcgagact gcgcacgtac cgttgcggtc agttgctgag
      721 tattcggtga aggcaatgcc cctattttcc cggcagtaac ctgcacgttt tgcgcttcaa
      781 tagcactttg cacgtctgaa ggcattagcg agtaagacgc cagtttggcc gggtcaagcc
      841 agatgcgcat agcgtattcc gcgccaaaga cttgcagact ccccacacct tcaacacgcg
      901 ccagcgggtc ctgaacgtta ctgaccaacc agtcggcgat atccgaactg gaggctttgt
      961 cagtggtgtc atacacggca gcaatcagca aaaagttgct ttgtgatttc tccaccgtaa
     1021 cacccgtttg ctgcacttcg ctgggtaggc gcgactccgc ctgctgaatt ttattctgca
     1081 cctgaacctg tgcagtatct ggatcggtac cttgttcaaa ggtcacatta atactgaccg
     1141 aaccatcaga gctactggtt gagctgaaat agagtaaatt atcaagccca gtgagttgtt
     1201 gctcgataac ctgagtcacg ctgttctcca gcgtttcggc agaagcacca gtataagtgg
     1261 ctgaaatttt aatggtcggc ggtgcaacgt caggatattg cgcgacgggt aatgtgcgaa
     1321 tagccagaat ccccgccaac ataatcaaaa tggcgataac ccaggcaaag accgggcggc
     1381 gaacaaaaaa gcgcgaaaac atcaggcgtt gcctccatta gcctgaactt caacagcctt
     1441 gacggtctgc cctgaagtga ctttggcaga accttcaaca atcagtcggt cgccgttgtg
     1501 caggccgttc agcaccagcc atttatcacc atacgtttct cccgtttcga gcgctcgctg
     1561 ctctacttta ttgtctttat tcaccaccag cgcagttgca ttgcctttag catcgcgcgt
     1621 gacgccctgt tgaggcgcga gaatagcgtc ttccatcacg ccttcatcga cacgagcgcg
     1681 aacaaacatt cccggtaata actgctgttg tggattgggg aaaattgccc gtaatgtcac
     1741 cgaaccggta gactcatcca ccgcgacttc ggtgagttcc agacgccctt tttcgctgta
     1801 ggttgtgcca tcttccagaa taagtgagac gctcatggtg tcactgttgg tcgccagtga
     1861 ctgtttacgc agacgtaata aatcgacact ggagcgagtg aggtcgacat acattgtatc
     1921 cagaccacga atcgtcgtta acgctgtatc ttgcgacgcg gtcaccagtg cgccaggggt
     1981 taccgacgaa atgccaatgc gccctgaaat cggtgcggtt accgtggtcc agtcaagatt
     2041 aatgcgcgca gtttccagtg cggctttttt cgcctctaca ctggctttat cttgtgcaca
     2101 ggtagactga gcatcatcag catcctgttg tgaaacaccg ttctctttca ccagtcgagc
     2161 ataacgctgc gctttctggc aatcggcttt taccagtgcc tgtgcttgtt gtaatgctgc
     2221 ccgggcttca ttccatgcag cctgataact ggccgcatca atctggtaga gcggctgtcc
     2281 agccttgacc agatcacctt ccttaaataa gcgtttctgg ataattcccc caacctgcgg
     2341 acgtacttcg gcactgagcg cagcactggt gcgtccggtt aattcactga ctaccgaaac
     2401 cggttgagat ttgagtgtaa caacgccgac ttctgtttct gacggggatg aattgttact
     2461 ttgcgtatta tcgcaacccg ataagagcag cattgccact acagatgttg ctatatactt
     2521 cattattttc tccagggtga gaaataccct cctcattgaa attaatgagg atatgtcagg
     2581 gatattcagt tataacgaac ccgcatcgcg aaatgaattt cgcaatgaaa taagggggat
     2641 taatatcccc cgctattggt taattctgtt cttatttatt tatcaacata ctaacaatat
     2701 cctgatatat ttcctgcaga tgttgtggat cgcttttttg cggtgttaaa cgacgataaa
     2761 ccgtaccttc catcatggta gcaaacactt caacgcagca acgaatgtgc tcgtcgctaa
     2821 gatggggatg cattcttttc atatgagcac aaccgttagc gaacattctg gcgtcagctt
     2881 cttcaagcat tgccatcacc cgcgggttgc gtgtggcttc tgcagccact tccagcatca
     2941 gcgcgtcgtc gtcctcattt aaggatttac gcaaggcaag aacttccgga aagtgatcgg
     3001 tacgcgcgtc aatgtccatt tgggcaatac gaaagtcgat gatacggcga accatctctt
     3061 caataatggc gtctttgttg gcgaagtaac gatatatctg acccacgcta agctgagctt
     3121 cgctggcaat ttgcgacata ctggcggcat gaaatccgca gcgacgaaaa caccgacggg
     3181 cggcgttaat gatttcatcc tgacgctgtt tgtggcgtat ctccagcttg ctggtcattt
     3241 ctgtctcctg gcctatcact tggtgagaat gatcattctc atttttgcta attatacgtt
     3301 tctccaaagt aatggaaagt atttttatct tattcatgat gcatattctg ataatgaaga
     3361 gattgtggag attctgtgaa gatagttgac aatattaagt tggggtcaat tcatcagaaa
     3421 gacagcgact tgttgtcgcc agttctgttt tcatcatcaa acttatcgga gctaatacga
     3481 atagtttcaa agtcagattc tataagatat ttccccaacc tcccccttgc aggcgtcagg
     3541 agtagataac gcaaaatgct ctgtaccgca gcttctctcc gctacagaaa gcgcaactat
     3601 agccacccac agccaggttg attataataa ccgtttatct gttcgtactg tttactaaaa
     3661 cgacgaatcg cctgattttc aggcacaaca agcatcaaca ataaggatta aagctatggg
     3721 ttttctttcc ggtaagcgca ttctggtaac cggtgttgcc agcaaactgt ccatcgccta
     3781 cggtatcgct caggcgatgc accgcgaagg agctgaactg gcattcacct accagaacga
     3841 caaactgaaa ggccgcgtag aagaatttgc cgctcaattg ggttctgaca tcgttctgca
     3901 gtgcgatgtt gccgaagatg ccagcatcga caccatgttc gctgaactgg ggaaagtttg
     3961 gccgaaattt gacggtttcg tacactctat tggttttgca cctggcgatc agctggatgg
     4021 tgactatgtt aacgccgtta cccgtgaagg cttcaaaatt gcccacgaca tcagctccta
     4081 cagcttcgtt gcaatggcaa aagcttgccg ctccatgctg aatccgggtt ctgccctgct
     4141 gaccctttcc taccttggcg ctgagcgcgc tatcccgaac tacaacgtta tgggtctggc
     4201 aaaagcgtct ctggaagcga acgtgcgcta tatggcgaac gcgatgggtc cggaaggtgt
     4261 gcgtgttaac gccatctctg cgggtccgat ccgtactctg gcggcttccg gtatcaaaga
     4321 cttccgcaaa atgctggctc attgcgaagc cgttaccccg attcgccgta ccgttactat
     4381 tgaagatgtg ggtaactccg cggcattcct gtgctccgat ctctctgccg gtatctccgg
     4441 tgaagtggtc cacgttgacg gcggtttcag catcgctgca atgaacgaac tcgaactgaa
     4501 ataatcgttc tgttggtaaa tattgggcgg cgttctgccg cccgttatct ctgttatacc
     4561 tttctgatat ttgttatcgc cgatccgtct ttctcccctt ctcgccttgc gtcaggataa
     4621 cgatttcctt tacgaccaag gagcgcctat ggaacaacgc cacatcactg gcaaaagcca
     4681 ctggtatcat gaaacgcaat ccagtactgc ggagtatgac gttctgcctc tggtcccgga
     4741 agccgcaaag gtcagcgatc cctttctgct cgacgtgatc cttgatgaag aaacgctggc
     4801 acccttcctt tcatggctgg tccctgcgcg cgttcttgta gtagagttgt tccctgacca
     4861 gcttaccgtg acccgttcac agaccttcac cgcttatgaa cgcttgtcga cggcccttac
     4921 ggttgctcag gtttgcggcg tccaacggtt atgtaactac tattcggcgc gacttacgcc
     4981 gctccccggg cctgattcct ccagggaaag taatcatcgg ttggcacaaa tcacgcaata
     5041 tgcccgccaa ctggctagct cgccttctat tatcgacaac cgatcgcgcc agcatctgaa
     5101 tgacgttggt cttactgcct gtgactgtgt gatcattaac caaatcattg gttttattgg
     5161 ctttcaggcg cggacaattg cgacatttca ggcttatctc gggcatccag tacgctggtt
     5221 acccgggctg gagatacaaa actacgccga cgcatcactg tttgctgatg aatcattacg
     5281 ctggcgaagc agctatgagg tggaaaaact ccctgaagag cacacaaaaa gttcaactgc
     5341 agaactttgc caactggcca acacactctc tctccaccct atttcacttt cccttctcga
     5401 aaagttgtta aacagcacac gggtcaatac acagccggat aatcagcttg cggcgttgtt
     5461 atgcgcgcgg ataaatggca gtcctgcttg ttttgccgcc tgtatgaatt catcaaatga
     5521 atataaaaaa atcagcctcc ttctgcgcaa gggcgaaaat gaaattaacc gatgggctga
     5581 ccgtcattct gttgagcgcg ctaccgttca ggcgatacaa tggctgaccc gagcacccga
     5641 tcgctttagc gccgcccagt tcagcccatt actcgaacac gaaaaatcat caacgcagat
     5701 tattaatctg ctggtatgga gcgggctgtg tggctggata aatcgtttaa aaatcgcgtt
     5761 gggtgagaca tattaacctt gccgcgtcag acagattcgc gtaaaactgt cagccgctct
     5821 aatggccacc aaaatagaca attatgtttc aggacaaccc gctgctagcg cagcttaaac
     5881 agcaactgca ttcccagacg ccacgcgctg aaggggtggt aaaagccaca gaaaaaggct
     5941 ttggcttcct ggaagtcgac gcgcaaaaaa gttatttcat tccgccgccg cagatgaaaa
     6001 aagtcatgca tggcgaccga attatcgcgg tgatccacag tgaaaaagaa cgtgaatccg
     6061 cagagccaga agaactggtt gaaccgttcc tgactcgttt cgtgggtaaa gttcagggca
     6121 aaaatgaccg tctggccatc gttcctgatc atccactctt aaaagacgcc attccttgcc
     6181 gcgcagcccg tggcctgaac cacgagttta aagaaggcga ctgggcggtt gccgaaatgc
     6241 gccgtcatcc gctgaaaggc gatcgttctt tctatgctga actgacacaa tacatcactt
     6301 ttggtgacga tcactttgta ccgtggtggg ttacccttgc gcgccataat ctggaaaaag
     6361 aagcaccaga cggcgtcgct accgaaatgc tcgatgaagg tctggttcgt gaagatctga
     6421 ctgcgctgga ttttgtcacc atcgacagtg ccagcacaga agatatggat gacgcccttt
     6481 tcgctaaggc gttgccggat gacaaacttc agctgattgt ggcgattgcc gatccaactg
     6541 cgtggattgc tgaaggcagc aagctggaca aagccgcgaa aattcgcgca ttcaccaact
     6601 atctgcctgg cttcaacatc cctatgctgc ctcgcgagct ttctgacgat ctctgctcac
     6661 tgcgcgccaa tgaagtccgc ccggtactgg catgccgcat gacgctctcc gctgatggca
     6721 ccattgaaga taatatcgaa ttctttgccg ccaccatcga atccaaagcg aagctggtgt
     6781 atgaccaggt ttctgactgg ctggaaaata ccggtgactg gcagcctgaa agcgaagcaa
     6841 ttgccgaaca agtccgtttg ctagcgcaaa tttgccaacg ccgcggcgag tggcgtcata
     6901 accacgcact ggtgtttaaa gatcgcccgg attaccgctt tattctcggt gaaaaaggag
     6961 aagtgctgga tatcgtcgcc gagcctcgtc gcattgccaa ccgtatcgtc gaagaagcga
     7021 tgattgccgc taacatttgt gcggcccgcg tactgcgcga taagctcggt tttggcatct
     7081 ataacgtgca tatgggcttt gatccggcga atgccgacgc gctggcagcg ttgctgaaaa
     7141 cccacggtct gcatgtcgat gccgaagaag tgctcacgct ggacggtttc tgcaaactgc
     7201 gtcgtgaact ggatgcgcaa ccaactggtt tcctcgacag ccgcattcgt cgcttccagt
     7261 catttgctga aattagcact gaacccggtc ctcactttgg cctcggtctg gaagcatacg
     7321 ccacctggac ttcgccgatc cgtaaatatg gcgacatgat caaccaccgt ctgctgaaag
     7381 cggttatcaa aggcgaaact gcgacgcgtc cacaggatga aatcactgtc caaatggccg
     7441 agcgtcgccg tctcaaccgg atggcagaac gtgatgttgg cgactggtta tacgcacgct
     7501 tcctgaaaga caaagccggg accgacaccc gtttcgcggc ggaaattgtc gatatcagcc
     7561 gtggcggcat gcgtgtgcgt ttggttgata acggcgctat cgcctttatt ccggcacctt
     7621 tcttacacgc tgtgcgcgat gaactggttt gcagccagga aaacggcact gtacaaatta
     7681 aaggtgaaac ggcttacaaa gtgactgacg ttattgacgt caccattgcc gaagtccgca
     7741 tggaaacccg cagcattatt gcgcgcccgg tcgcgtaatc ttctttcacg gcccattcct
     7801 catgaatggg ccgtttattt ccccgctcca ccttcgtcat atcccggcag atttttaact
     7861 gactttcgtt tgaaaactgg cgtttttcca aaactggatt acaaataaat atataaaaaa
     7921 aacaatgcgt ttagccgccg ttaatttcat gtaagtcatg ttgttccttt tatttaaccg
     7981 cggactccgc agttaaccgg aggatatgca tcatgaaaac cgttagggag tccacaacgt
     8041 tgtacaactt tctcggatcg cacaatccat actggcggtt aacggaaagc agcgatgttt
     8101 tgcgcttttc taccaccgaa accacagaac ctgaacgtac gttgcagtta tctgccgaac
     8161 aggctgctcg catcagggaa atgacggtca tcacctccag cctgatgatg agtctgaccg
     8221 tcgatgaaag cgatctttct gtgcatctgg taggacgaaa aatcaataaa cgggaatggg
     8281 ctggcaacgc gtctgcatgg catgacacac cagcagttgc tcgtgattta tcacacgggc
     8341 tttcctttgc tgagcaggta gtttctgaag cacattccgc aatagtgatt ctcgacagcc
     8401 gggggaatat ccaacgcttc aatcggttat gtgaagatta cactgggttg aaagaacacg
     8461 acgtcattgg ccaaagcgtg tttaaactgt ttatgagccg tcgtgaagct gcggcatcca
     8521 ggcgcaataa ccgtgtattt tttcgaagcg gcaatgcata tgaagtcgaa ctgtggatac
     8581 caacacgtaa aggccagcgg ctgtttctgt ttcgcaataa atttgtccac agcggcagtg
     8641 gcaaaaacga gattttttta atctgttccg gcaccgacat taccgaagag cgccgcgctc
     8701 aggagcgact gcgtattctg gcaaataccg acagtatcac cggactgccg aatcgtaatg
     8761 caatgcagga gttaatcgat cacgctatta atcaggcaga taacaataaa gttggggttg
     8821 tgtatcttga tttggataat tttaaaaagg tcaacgacgc ctatgggcat ttatttggtg
     8881 accagttatt acgcgacgtg tcattggcca ttttaagctg tctcgaacat gatcaggtgt
     8941 tggcgcgtcc aggtggcgat gagtttctgg tactggcatc caatacctca caaagcgcgc
     9001 tggaagcaat ggcatcacga attttgaccc gcttacgact ccccttccgc attggtttaa
     9061 ttgaagttta taccagctgt tcagtaggta tttcactctc tcccgaacat ggttcggaca
     9121 gcgcggctat tattcgtcac gccgacacag caatgtacac agcgaaggaa ggcggacgag
     9181 gacaattttg cgtttttacc ccagaaatga atcagagggt atttgaatat ctctggctgg
     9241 ataccaactt gcgtaaagca ctggaaaacg atcagttggt tattcactat caaccgaaaa
     9301 tcacctggcg tggcgaagtg cgcagtctgg aagcactagt acgttggcag tcacctgaac
     9361 gtgggttgat tccaccgttg gacttcattt cctacgccga agagtcaggg ctaattgtgc
     9421 ctttaggccg ttgggtgatt ctcgatgtcg tacgccaggt ggcaaagtgg cgggataaag
     9481 gtattaacct gcgagtggcg gtaaacattt ctgcacgtca gctcgccgat caaaccattt
     9541 tcaccgccct gaaacaggtt ctccaggaac tcaattttga atactgccct attgatgttg
     9601 aactgacgga gagctgcctg attgagaatg atgaactggc actgtctgtt attcaacaat
     9661 ttagccaact aggtgcgcaa gtgcatctgg acgattttgg taccggctac tcttcacttt
     9721 cgcaactggc gcgctttccg atcgatgcca tcaaacttga ccaggttttt gttcgggata
     9781 ttcacaaaca gcctgtctcg cagtcactgg tccgggcgat cgtcgctgtg gcacaggcat
     9841 tgaatcttca ggtgatcgct gaaggagtgg agagtaaaaa ggaagatgct tttttaacca
     9901 agaacgggat caatgagcgg caaggatttt tgtttgccaa accgatgccc gccgtcgcct
     9961 tcgaacgctg gtataaacgc tatctgaagc gcgcataagt agatgaatat atatacttag
    10021 cgatatattc cgtcattata tatcgtatta ctggatgata attagccgtt gctgttaaac
    10081 acttctcttt tagcggcaat ttttgcatta ttcattttcc cccttttgct ataaggatgt
    10141 ccttgatgtc tgaatttgat gcccagcgcg ttgccgaacg tattgatatt gtgttagata
    10201 tccttgtggc aggcgattac cactctgcga tccataatct cgagatactt aaagcagaac
    10261 tcttgcgcca ggttgctgaa tctacgccag acatcccgaa ggcaccgtgg gagatttaac
    10321 aatcgtgcct tcaggttttc ctatttgagg atgcccggcg cgtcagtatt tttttattta
    10381 gtattataac gttataagaa ttacagcgat gaattcccga caacaaacta ttctacagat
    10441 ggtcattgac cagggtcagg ttagcgtaac cgatctggca aaagccactg gagtttctga
    10501 agttaccatt cgccaggatc tcaacaccct cgaaaaactg agttacctcc gccgtgcaca
    10561 tggctttgca gtatcgcttg atagtgatga cgtcgaaact cgtatgatga gcaactatac
    10621 gctgaagcgt gaactcgctg agtttgccgc gtcactggtt caaccgggcg aaaccatctt
    10681 tatcgaaaat ggcagcagca atgccctgct cgcccggact ctgggcgagc agaagaaaaa
    10741 tgtcacgatc atcacggtca gcagctacat cgcgcatttg ctgaaagacg cgccttgtga
    10801 agttattttg ctcggtggcg tgtaccagaa aaaaagcgaa agtatggttg gtcctttgac
    10861 acgccagtgc atccaacagg tgcatttcag caaagcattt attggtattg atggctggca
    10921 acctgaaact gggtttaccg gtcgcgatat gatgcgtacc gatgtggtca atgccgtgct
    10981 ggaaaaagag tgcgaggcga tagtcctgac tgacagctcg aaatttggtg ctgtacattc
    11041 atactccatc ggccccgttg agcgattcaa tcgcgtgatt accgattcga aaatacgcgc
    11101 cagcgatctg atgcatcttg agcacagtaa actcaccgtt cacgtcgttg acatttaacc
    11161 atttcccttc tctacggatg atttgctgtt ttgcaaatca tccgctctaa gatgattcct
    11221 ggttgataat taagactatt tacctgttat taacactctc aagatataaa attattatca
    11281 gcgatataac aggaagtcat tatcacctgc gtgatataac cctgcgcgcg agcggatttc
    11341 acggaataat ttcaccaggc ttattcttag ctattatagt tatagagagc ttacttccgt
    11401 gaatcataaa ttcaggagag agtattatgt ttgtaacgag caaaaaaatg accgcggctg
    11461 ttctggcaat taccctggca atgtctctga gtgcctgttc taactggtct aaacgagacc
    11521 gtaacaccgc aattggcgcg ggcgcaggtg cattaggcgg tgcagtactg accgatggca
    11581 gtacgctggg tacattaggt ggtgcagccg tcggtggtgt tattggtcac caggtcggta
    11641 aataatcgta aataaatgtc gattatcgac tcgcaattat aatacacttg attctgttca
    11701 gtacgaaata actgaataat acacacagta aaacattcga aagccacgga tatatccgtg
    11761 gctttttatg ttagccgcct gcgagtttta ccttcatccc tttcgcttca agcagtgatt
    11821 ttaataaatc acgcttatcg ccctggattt caataattcc atctttaact gctccgccgc
    11881 agccgcattt tttcttcagt tccgctgcca gtttcgtcag ttcggcatca tcgagatcga
    11941 caccggtaat caggcaaacg cccttacctt tacgtccact ggtctgacgc tgaatacgca
    12001 ccacaccgtc gcctttaggg cgtacagggg ccgcttttgg ctcatcaata cgtccggttt
    12061 ccgttgagta gaccagacgg ctgttggaat cactcatcat gcactccgct gtaaagaggc
    12121 gttgatcgct ttcagcgtct gcgctggatc taccgattgc gttaccgggc gaccaatcac
    12181 catataatca acaccagccg ccaacgcctg ttctggcgtc ataatgcggc gctggtcacc
    12241 tgcttcactc ccctgcggac gaatgcccgg tgtaaccagt ttgaactcct gaccgaatac
    12301 ctgtttaaag cgcacagctt cctgagcaga acacaccaca ccatcaaggc cacatttttg
    12361 cgtcagtgcc gccagacgtt ctgcataatc tgcaggtgac agtgtcacgc caagatcggc
    12421 caggtcgctg gcttccatgc tggtcaatac tgtcacagca atcaaaagcg gtgcatcttt
    12481 gccaaacgga accagtgcct cacgcgctgc ggtcatcata cgcgccccac cggaggcgtg
    12541 aacattcacc atccacacgc ctaagtcagc tgcagcagcg acagcgtgcg ctgcagtgtt
    12601 gggaatatcg tgaaatttca ggtcgaggaa gatatcaaaa ccacgctgtt gaagttcgcg
    12661 tacaaattga ggtccaaaca gcgtaaacat ctctttgccg actttcagac gacaatcgcg
    12721 tgggtcgatc ttgtcgacaa aggccagcgc ggcatcacga ttatgataat caagggcaac
    12781 aaccacagga gaattcgtaa cagcgcggga agaagatgaa gcagttaacg tcatgaccag
    12841 accttcttga tgatgggcac cggaagtggc gcgaaacagg taaacggcga gcattctacc
    12901 cgtggaaata aaaaattaac agttgcgatt tctcccctgg caggcttttc tgcgcccgag
    12961 ctaccggagc acaatggaac atcaacaatt agtatgttgt aactaaagtc ggattttttt
    13021 aaaaattaca ggccatcaag accgcgaatc ggtttaatgg ttgaccaggc ccgacaagac
    13081 ggacaatgcc agtagagagt gtatgcggta aacccacatt tctggcagcg ataacgaggc
    13141 ttactccgca ccttctcgcc aaccatgtca cgcagcacca tcagactctc tttggcacgc
    13201 ccttcttccg cttcatttaa gtggtaatcc attagcttat ggaacacacg catggtcgga
    13261 tgacgctgaa gctgacgcgt aatatagacc tgtgcggcct cactaccgtc gcgcgcttcg
    13321 atgatgtccg caagcatcag ttcagcatcg gcaccggtgt tctcttccac cgcgcgctgc
    13381 aggaactctg cccattcggc agttttaccc aactgctggt agcaggtttg cagcatttcc
    13441 agcgtttcgc tgaccagttc tctgtcctgg gatatgacac gttgcagact ttcgacggct
    13501 ttggcgtatt ctcctttcgc cataaacacg cgtcccatca tgatggatac gcgggcgctg
    13561 tttttatctg ccgccgcacc tttttttagc aaagtcatgg cacgatcgag atcgtcgctg
    13621 gccatatgct gcagagctaa ctcacagtag aaatgggcaa tttcgacgcg ctgtttatct
    13681 ttacccagct tcaccaggcg ttcggcaaca tcaattgctt tctgccactc gctggtagcc
    13741 tggtagattt gtagcaactg ttgcagcgcg ccaatgcgga agtcagtttc atcggtcagc
    13801 tgattgaaca tgtcttccgc gcggtcgtat aacccggcag ccatgtaatc acgccccagt
    13861 tgttgaatcg ccaaaagccg ctgttcatag gtcagcgagg cgctttccat tagggtctga
    13921 tggatgcgaa tagcgcgatc aacttcgcca cgcgaacgga acaggtttcc gagcgtaagg
    13981 tgggcttcaa cggtacctgt atcctcttta agcatatcga gaaacagatc taccgcttta
    14041 tcctgttgat tactaagcag gaagttaacc cccgctacgt aatcacgcga caagcggttg
    14101 gcttcatctt gcttgttttg ttgcgcactt ctgcggccca tataccagcc ataggcagcg
    14161 gctacaggca aaagcagaaa caacaactcc agcatagaaa gttattcctt cgccgctgac
    14221 gagtgcggcg ctacagccac gtcagtcgcg ggtgaaagct ggttttccag tcgctttatt
    14281 ttacgttcag cgcgcgccag ggaaacacga actcgcagcc agaacaggcc acaaatcaac
    14341 caaccgatag caaaccccgc agcaaacaat accgccagca atgtggaaat acggtactcc
    14401 ccttgcgcta acagataatt aaacgtcacc tgttgatcgt tctgcgcacc taacgtaacc
    14461 gaaatcacga agatcgctaa caccagtaag aaaatgagta aatatttcac attacttccc
    14521 gttatgtggt tagagcaaat aaattatggt caacttaccg caatagccct ttaaagtacc
    14581 attttcgcgc cgtctgcgaa atggcatcca cgcgcattgt tgatgattta agggctaaat
    14641 tcggaaaatc aaccagcgtt aactttcttg ttctcgttgc gctatttcgc gattttcttc
    14701 cgcaggtggt gttaatggcc cacaaattcg ttgcgcaagc cacgttgcca ccgccaccag
    14761 cgcccacgaa atcaacgtag ctactaccag atcgcgtggc caatgcatcc cgagcagcag
    14821 gcggcttccc atgactccag ttgcccagac caacaagata gcaatggtta acgttcgccg
    14881 acgcggccac agcaaaccaa cggccagcag tgcccaactg gcagcaaaca tcgtgtgacc
    14941 ggaaggaaag gcaaaccccg tctctttctg ccagtgtgaa cgcaaatatt gtgggatatt
    15001 tttctcttca gccaactgtt ctttcactag atttccgcgt tctgctcgct ttaaagtgta
    15061 gaactcatca accggaatat gatgtgtttt ttccagccag ataacaaaag gtcgtggttc
    15121 ctggactttg tctttgatcc aggatttaac gccttgtccc acaaggattg ccgccgccag
    15181 aatggcaaat aatacaaagg cagccttaat gcgaaaacgc agacaccaga gaaaccagcc
    15241 gaataaaatc aaatgtgtaa tgacgcccca gggctgggtg acagtttcag taacccaaaa
    15301 agccgctttt agtaaccaac tttgttctcc aggttgccaa cgccagccag aaatccatac
    15361 ggctactggc ataacaagca atagtgcagt tcccactgcg gtacgtctgg caatcgaacg
    15421 catggcctct cctttttgat aagtcccaca atcataactg aaaatgccag ttccaggaaa
    15481 aattgacaga tttgtgccat tccgtgaacg atcgacgcgt cgtgattagg tgaacccctt
    15541 ctcgttatgg caaaataagc caatacagaa ccagcattat ctggagaatt tcatgcagct
    15601 taaacgtgta gcagaagcca aactgccaac cccatggggc gatttcctga tggtgggatt
    15661 tgaagaactg gcaaccggac acgatcatgt cgcgctagtc tatggcgata tttccgggca
    15721 taccccggta cttgcgcgcg tccattccga atgtctgacc ggtgacgccc tgttcagctt
    15781 gcgctgcgat tgtggcttcc agctcgaagc ggcactgacg caaattgccg aagaaggccg
    15841 tggtattttg ctgtatcacc gtcaggaagg tcgtaacatt ggtctgctga ataaaatccg
    15901 cgcttatgca ctgcaggatc aaggttacga tactgtagag gctaaccacc agttaggctt
    15961 cgccgctgat gagcgcgact tcactctttg cgctgatatg ttcaaactcc ttggcgtcaa
    16021 tgaagtccgc ttgttaacca acaacccgaa aaaagtcgaa attctgaccg aagcagggat
    16081 taatattgtt gaacgcgtac cgttgattgt aggtcgtaac cccaataacg aacattatct
    16141 cgataccaaa gccgagaaaa tgggtcattt gctgaacaaa taaccctctt gcattgtgta
    16201 attcatttgc ttgccggaag caaaataacc ggcaagcaaa tactcgttac ttcaacatat
    16261 tacgaatgac ataatgcaaa atgccgtcgt tctggtagta ggtcaactcc gtcgcggtgt
    16321 cgatacgaca acggcagggt acgacttcct ggctaccatg cgcgcgcgta agcgtcaccg
    16381 gaaccgtcgc gccgggctgt agattttgca gatcgccaat atcaatcttc tcttccccgg
    16441 ttagccctaa cgttttacgc gttacgcctt gcggaaattc cagcggcagg atgcccatgc
    16501 caattaaatt cgaacggtga attcgttcaa acgattcggc aatcaccaca cgaatgccaa
    16561 gtagacgcgg accttttgcc gcccagtcac ggctggagcc tgatccatac tctttcccgg
    16621 caatcaccgc cagcggcgtt tgctcctgct tatagcgcat cgcggcatca taaatagaga
    16681 tgacctcgct gtcaggtaaa tgccgcgtca tccccccttc aacgccaggc accatttcat
    16741 tacggatgcg aatattggcg aaggtgccgc gcatcatcac ttcatggtta ccacgccgcg
    16801 aaccgtagga gttaaagtct ttgcgctcaa caccccgacc ttgtagatat cgccccgccg
    16861 ggctatcggg cttaatgctg cccgccggag agatatggtc agtggtgact gaatccccca
    16921 gcattgcgag gatccgcgca ccgtgaatat cttccacagg tgctggtgtt gcctgcattt
    16981 catcaaagaa aggcgataag cgaatatagg ttgaatcctc ctgccaaccg taggtatcgg
    17041 atcgtgcgac gttaatttcc ttccactctg ctgtgccttc aaaaacttct gcgtactctt
    17101 tacggaacat ttctgtggag acttgttcta ccgcacgggc aatttcttgt gccgagggcc
    17161 agatatcttt cagatatacc gggtcgcctt tacgatcgtg accaataggc tcagaagcga
    17221 ggttaatatt catatttccc gctagcgcat aggcaaccac cagcggcggc gaggccagcc
    17281 agttagtttt aaccagcgga tggatacggc cttcaaagtt acggttgccg gacagcaccg
    17341 caccgacggt taaatcgcct tttttgattg ccgtttcgat aggatcgggc aatggaccag
    17401 agttaccaat acaggtggta caaccgtatc ccacaaggtt aaaccccagt tcatcgagat
    17461 acggtgtcag tttcgctttt gccaaataat cagaaacgac tttcgagcct ggtgccagcg
    17521 acgctttgac ccacggttgc cgcttgaggc ccagagttac agcttttttc gccagcaaac
    17581 ctgcggccat cagcacactt gggttagagg tgttggtgca cgacgttatc gcagcaatga
    17641 ccaccgcgcc atcaggtaac tgatactgat gtccgttcat aacataatcg accggctggc
    17701 gatctttatg cgtggcattt acttccagtt cgttactggc ggcaaatgct tttggtacat
    17761 cgggcagtgc aacgcgatcc tgtgggcgtt ttggccctgc caggctcgct tcaacgtcat
    17821 tcatatccag ttctaacgta ctggtaaaaa tcggttcatc gcccgggtta cgccacatgc
    17881 cctgcgcttt ggcatatttt tcgaccaact cgacctgatc ttcgctgcgc ccgcttaaac
    17941 gcatgtaatc gagggtgaca gcatcgattg ggaagaagcc acaggtggca ccatattctg
    18001 gcgacatatt ggcaatggtg gcgcgatccg ccagcggtag tgaatccaga ccatcaccat
    18061 aaaattcgac gaatttcccc accacgccat gtttgcgcag catttgggta acggtgagaa
    18121 ccaggtctgt ggcggtaata ccttcacgta attttccggt aagtttaaag cctactacgt
    18181 ccgggataag catggaaacc ggctggccta acattgcggc ttcggcttcg atcccaccaa
    18241 cgccccaccc cagcacgcca aggccgttga tcatggtggt gtgcgagtca gtaccaacga
    18301 gtgtatccgg ataagcaatc cattcaccgt cctgcaattc gctccacact gctttgccga
    18361 gatattcgag gttaacctga tggcaaatgc ctgtgcctgg cggcacgacg ctaaaacgac
    18421 tgaacgcctg ctttccccat ttcaggaaca cataacgttc gtggttgcgc tccatttcca
    18481 ggcgcacgtt ttcttcaaat gcctcatcat caccaaaacg atcgacggtc accgagtggt
    18541 caatgaccag gtcgaccggt gagagcgggt taacctttgc agtatcgccg ccgaggcgtt
    18601 taaccgcttc gcgcattgcc gccagatcaa caacggcagg tacgccggta aagtcctgca
    18661 tcagcactct tgccgggcgg taggcaattt cacggtcagc atgggcattt ttcagccatc
    18721 ccgccagcgc gtggatatcc tcttcggtaa ccgatttacc atcctgccaa cgcagcaggt
    18781 tttcgagcaa aactttgagt gaattgggta gacgggtgat atcgcccagt gatttggcag
    18841 caagcggcaa gctgtagtag tggtaagttt tatctttggc ctgcaacgtg tccttactgg
    18901 cttctcgtag ggttgacgac atagctcctc cttaatgaca gggttgcgaa gccctgattc
    18961 tcttcagggt gaatattaaa gataacacaa acatcgcgta acgatttgat aacaacccaa
    19021 attgataaaa gaggagaaac cttgagacaa agcaaacagc ggggtcaggg atcagtgagt
    19081 aatgatccag aggagttcgg cccagaagag catcgacaga gaaaatgcgc ccatccagga
    19141 ccaatatttc atatttgtgt tgagataact caacctgtta ttgctgcggt gtattaaaag
    19201 ccctgtgatt cattaaaaat gggtaataag aatgatcata attattgttt cggctgatca
    19261 tcttcagcaa acagttctat aaaggcttgt tgctgcgatg ttagttgcca gtcgtctgga
    19321 ataacgacgg aacattcccg ttttgcttct ggttcattgt cactatgctg acataatggt
    19381 gacgactcca gttgctcctg accgaccata gttatcccca cactttccaa ataagcagtg
    19441 cgacaaccac ccaggaaagt gcagaaccaa gaaaaaccgc cagccaggct ttacgtttta
    19501 attcaggatt gcggcggggt tcctgatttc cagaaggcat tgctaacctc atataatcgc
    19561 catcacttat caacaagacg ttgaacgatt ggtgtaatta atcatacgat gagacaaatc
    19621 ctaaagaaac tttagtcaaa aatccagcga atttacgaat cgtctccagt gaataattca
    19681 atcttttgac ctgaaataga tatttgaaaa ctggagtttg cgaggcgggt aattagacac
    19741 tactttacag gtaatttacc ctataaatat gagggataaa aatattgtgc acggtataca
    19801 aataagtaac aaaagagaat atattccggc accttcgcta cataaaaggt gccgaaaata
    19861 acgcaagaaa ttatttttcc ggcagtttta tatctttaaa catggcctca atttcttcat
    19921 tagagcgcaa tgcgacagcc gcatcaacga catcacgcgt taaatgcggt gcaaaacgct
    19981 gaatgaaatc atacatataa ctgcgcaaaa aagtactacg gcgaaaacca attttggttg
    20041 tactgtggct gaagatatcg tgagcatcaa cgcgcaccag gtcgg
//
LOCUS       DAMZFT010000088        19667 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.088, whole genome shotgun sequence.
ACCESSION   DAMZFT010000088 DAMZFT010000000
VERSION     DAMZFT010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19667)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19667)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19667
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.088"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..2810
                     /gene="cusA"
                     /locus_tag="PN977_003308"
     CDS             <1..2810
                     /gene="cusA"
                     /locus_tag="PN977_003308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter permease
                     subunit CusA"
                     /protein_id="HDI6043313.1"
                     /translation="YLNQVQGKLPAGVSAELGPDATGVGWIYEYALVDRSGKHDLADL
                     RSLQDWFLKYELKTIPDVAEVASVGGVVKEYQVVIDPQRLAQYGISLAEVKSALDASN
                     QEAGGSSIELAEAEYMVRASGYLQTLDDFNHIVLKASENGVPVYLRDVAKVQVGPEMR
                     RGIAELNGEGEVAGGVVILRSGKNAREVIAAVKDKLETLKSSLPEGVEIVTTYDRSQL
                     IDRAIDNLSGKLLEEFIVVAVVCALFLWHVRSALVAIISLPLGLCIAFIVMHFQGLNA
                     NIMSLGGIAIAVGAMVDAAIVMIENAHKRLEEWQHQHPDATLDNKTRWQVITDASVEV
                     GPALFISLLIITLSFIPIFTLEGQEGRLFGPLAFTKTYAMAGAALLAIVVIPILMGYW
                     IRGKIPPESSNPLNRFLIRVYHPLLLKVLHWPKTTLLVAALSVLTVLWPLNKVGGEFL
                     PQINEGDLLYMPSTLPGISAAEAASMLQKTDKLIMSVPEVARVFGKTGKAETATDSAP
                     LEMVETTIQLKPQDQWRPGMTMDKIIEELDNTVRLPGLANLWVPPIRNRIDMLSTGIK
                     SPIGIKVSGTVLADIDTMAEQIEEVARTVPGVASALAERLEGGRYINVEINREKAARY
                     GMTVADVQLFVTSAVGGAMVGETVEGIARYPINLRYPQSWRDSPQALRQLPILTPMKQ
                     QITLADVADVKVSTGPSMLKTENARPTSWIYIDARDRDMVSVVHDLQKAIAEKVQLKP
                     GTSVAFSGQFELLERANHKLKLMVPMTLMIIFVLLYLAFRRVGEALLIISSVPFALVG
                     GIWLLWWMGFHLSVATGTGFIALAGVAAEFGVVMLMYLRHAIEAEPSLNNPQTFSEQK
                     LDEALYHGAVLRVRPKAMTVAVIIAGLLPILWGTGAGSEVMSRIAAPMIGGMITAPLL
                     SLFIIPAAYKLMWLHRHRVRK"
     gene            2912..4288
                     /gene="pheP"
                     /locus_tag="PN977_003309"
     CDS             2912..4288
                     /gene="pheP"
                     /locus_tag="PN977_003309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine transporter"
                     /protein_id="HDI6043314.1"
                     /translation="MKNASTVSEDTASNQEPTLHRGLHNRHIQLIALGGAIGTGLFLG
                     IGPAIQMAGPAVLLGYGVAGIIAFLIMRQLGEMVVEEPVSGSFAHFAYKYWGPFAGFL
                     SGWNYWVMFVLVGMAELTAAGIYMQYWFPDVPTWIWAAAFFIIINAVNLVNVRLYGET
                     EFWFALIKVLAIIGMIGFGLWLLFSGHGGEKASIDNLWRYGGFFATGWNGLILSLAVI
                     MFSFGGLELIGITAAEARAPEKSIPKAVNQVVYRILLFYIGSLVVLLALYPWVEVKSN
                     SSPFVMIFHNLDSNVVASALNFVILVASLSVYNSGVYSNSRMLFGLSVQGNAPKFLTR
                     VSRRGVPINSLMLSGAITSLVVLINYLLPQKAFGLLMALVVATLLLNWIMICLAHLRF
                     RAAMRRQGRETQFKALLYPFGNYLCIAFLGMILLLMCTMDDMRLSAILLPVWIVFLFV
                     AFKTLRRK"
     gene            complement(4356..5603)
                     /gene="ybdG"
                     /locus_tag="PN977_003310"
     CDS             complement(4356..5603)
                     /gene="ybdG"
                     /locus_tag="PN977_003310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel YbdG"
                     /protein_id="HDI6043315.1"
                     /translation="MQDLISQVEDLAGIEIDHTTSMVMIFGIIFLTAVVVHIFLHWVV
                     LRTFEKRAIASSRLWLQIITQNKLFHRLAFTLQGIIVNIQAVFWLQKGTEAADILTTC
                     AQLWIMMYALLSVFSLLDVILNLAQKFPAASQLPLKGIFQGIKLIGAILVGILMISLL
                     IGQSPAILISGLGAMAAVLMLVFKDPILGLVAGIQLSANDMLKLGDWLEMPKYGADGA
                     VIDIGLTTVKVRNWDNTITTIPTWSLVSDSFKNWSGMSASGGRRIKRSISIDVTSIRF
                     LDEDEMQRLNKAHLLKPYLTSRHKEINEWNRQQGSTESVLNLRRMTNIGTFRAYLNEY
                     LRNHPRIRKDMTLMVRQLAPGDNGLPLEIYAFTNTVVWLEYESIQADIFDHIFAIVEE
                     FGLRLHQSPTGNDIRSLAGAFKQ"
     gene            complement(5711..6364)
                     /gene="nfsB"
                     /locus_tag="PN977_003311"
     CDS             complement(5711..6364)
                     /gene="nfsB"
                     /locus_tag="PN977_003311"
                     /EC_number="1.5.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-insensitive NAD(P)H nitroreductase"
                     /protein_id="HDI6043316.1"
                     /translation="MDIISVALKRHSTKAFDASKKLTPEQAEQIKTLLQYSPSSTNSQ
                     PWHFIVASTEEGKARVAKSAAGNYVFNERKMLDASHVVVFCAKTAMDDAWLKLVVDQE
                     DADGRFATPEAKAANDKGRKFFADMHRKDLHDDAEWMAKQVYLNVGNFLLGVAALGLD
                     AVPIEGFDAAILDAEFGLKEKGYTSLVVVPVGHHSVEDFNATLPKSRLPQNITLTEV"
     gene            complement(6443..6826)
                     /locus_tag="PN977_003312"
     CDS             complement(6443..6826)
                     /locus_tag="PN977_003312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000360941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HDI6043317.1"
                     /translation="MDKQSLHETAKRLALELPFVELCWPFGPEFDVFKIGGKIFMLSS
                     ELRGVPFINLKSDPQKSLLNQQIYPSIKPGYHMNKKHWISVYPGEEISEALLRDLIND
                     SWNLVVDGLAKRDQKRVRPDSTVNQ"
     gene            complement(6891..7139)
                     /locus_tag="PN977_003313"
     CDS             complement(6891..7139)
                     /locus_tag="PN977_003313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1158 domain-containing protein"
                     /protein_id="HDI6043318.1"
                     /translation="MKHPLETLTTAAGILLMAFLSCLLLPAPALGLTLAQKLVTTFHL
                     MDLSQLYTLLFCLWFLVLGAIEYFVLRFIWRRWFSLAD"
     gene            complement(7205..8323)
                     /locus_tag="PN977_003314"
     CDS             complement(7205..8323)
                     /locus_tag="PN977_003314"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HDI6043319.1"
                     /translation="MPLPDFHVSEPFTLGIELEMQVVNPPGYDLSQDSSMLIDAVKNK
                     ITAGEVKHDITESMLELATDVCRDINQAAGQFSAMQKVVLQAAADHHLEICGGGTHPF
                     QKWQRQEVCDNERYQRTLENFGYLIQQATVFGQHVHVGCASGDDAIYLLHGLSRFVPH
                     FIALSAASPYMQGTDTRFASSRPNIFSAFPDNGPMPWVSNWQQFEALFRCLSYTTMID
                     SIKDLHWDIRPSPHFGTVEVRVMDTPLTLSHAVNMAGLIQATAHWLLTERPFKHQEKD
                     YLLYKFNRFQACRYGLAGVITDPHTGDRRPLTEDTLRLLEKIAPSANKIGASSAIEAL
                     HRQVVSGLNEAQLMRDFVADGGSLIGLVKKHCEIWAGD"
     gene            8765..8917
                     /gene="hokE"
                     /locus_tag="PN977_003315"
     CDS             8765..8917
                     /gene="hokE"
                     /locus_tag="PN977_003315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokE"
                     /protein_id="HDI6043320.1"
                     /translation="MLTKYALVAVIVLCLTVLGFTLLVGDSLCEFTVKERNIEFKAVL
                     AYEPKK"
     gene            complement(9039..9659)
                     /gene="entD"
                     /locus_tag="PN977_003316"
     CDS             complement(9039..9659)
                     /gene="entD"
                     /locus_tag="PN977_003316"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin synthase subunit EntD"
                     /protein_id="HDI6043321.1"
                     /translation="MKTTHTALPFAGNTLHFVKFDPASFCEQDLLWLPHYAQLQHAGR
                     KRKTEHLAGRIAAVYALREYGYKCVPAIGELRQPVWPAGVYGSISHCGTTALAVVSRQ
                     PIGIDIEEIFSAQTAAELTDNIITPAEHKRLADCGLAFSLALTLAFSAKESAFKACER
                     QTDAGFLDYQIINWNKQQVIIHRENKMFAVHWQIKEKIVITLCQHN"
     gene            complement(9834..12074)
                     /gene="fepA"
                     /locus_tag="PN977_003317"
     CDS             complement(9834..12074)
                     /gene="fepA"
                     /locus_tag="PN977_003317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore enterobactin receptor FepA"
                     /protein_id="HDI6043322.1"
                     /translation="MNKKIHSLALLVNLGIYGVAQAQEPTDTPVSHDDTIVVTAAEQN
                     LQAPGVSTITADEIRKNPVARDVSEIIRTMPGVNLTGNSTSGQRGNNRQIDIRGMGPE
                     NTLILIDGKPVSSRNSVRQGWRGERDTRGDTSWVPPEMIERIEVLRGPAAARYGNGAA
                     GGVVNIITKKGSGEWHGSWDAYFNAPEHKEEGATKRTNFSLTGPLGDEFSFRLYGNLD
                     KTQADAWDINQGHQSARAGTYATTLPAGREGVINKDINGVVRWDFAPLQSLELEAGYS
                     RQGNLYAGDTQNTNSDAYTRSKYGDETNRLYRQNYSLTWNGGWDNGVTTSNWVQYEHT
                     RNSRIPEGLAGGTEGKFNEKAAQDFVDIDLDDVMLHSEVNLPIDFLVNQTLTLGTEWN
                     QQRMKDLSSNTQALTGTNTGGAIDGVSATDRSPYSKAEIFSLFAENNMELTDSTIVTP
                     GLRFDHHSIVGNNWSPALNISQGLGDDFTLKMGIARAYKAPSLYQTNPNYILYSKGQG
                     CYASAGGCYLQGNDDLKAETSINKEIGLEFKRDGWLAGVTWFRNDYRNKIEAGYVAVG
                     QNAAGTDLYQWDNVPKAVVEGLEGSLNVPVSETVMWTNNITYMLKSENKTTGDRLSII
                     PEYTLNSTLSWQAREDLSMQTTFTWYGKQQPKKYNYKGQPAVGPETKEISPYSIVGLS
                     ATWDVTKNVSLTGGVDNLFDKRLWRAGNAQTTGDLAGANYIAGAGAYTYNEPGRTWYM
                     SVNTHF"
     gene            complement(12079..12279)
                     /locus_tag="PN977_003318"
                     /pseudo
     CDS             complement(12079..12279)
                     /locus_tag="PN977_003318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001174912.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            12317..13519
                     /gene="fes"
                     /locus_tag="PN977_003319"
     CDS             12317..13519
                     /gene="fes"
                     /locus_tag="PN977_003319"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterochelin esterase"
                     /protein_id="HDI6043323.1"
                     /translation="MTALKVGSESWWQSKHGPEWQRLNDEMFEVTFWWRDPQGSEGHS
                     TIKRVWVYITGMTDHHQNSQPRSMQRIAGTDVWQWTTQLNANWRGSYCFIPTERDDIF
                     SAPSPDRLELREGWRKLLPQAIADPLNPQSWKGGRGHAVSALEMPQAPLQPGWDCPQA
                     PETPAKEIIWKSERLKNSRRVWIFTTGDATAEERPLAVLLDGEFWAQSMPVWPALTSL
                     THRRQLPPAVYVLIDAIDTTHRAHELPCNADFWLAVQQELLPQVKAIAPFSDRADRTV
                     VAGQSFGGLSALYAGLHWPERFGCVLSQSGSYWWPHRGGHQEGMLLEQLNTGEVSAEG
                     LRIVLEAGVREPMIMQANQALYAQLHPLKESIFWRQVDGGHDALCWRGGLMQGLIDLW
                     QPLFHDRS"
     gene            13522..13740
                     /gene="ybdZ"
                     /locus_tag="PN977_003320"
     CDS             13522..13740
                     /gene="ybdZ"
                     /locus_tag="PN977_003320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin biosynthesis protein YbdZ"
                     /protein_id="HDI6043324.1"
                     /translation="MAFSNPFDDPQGAFYILRNAQGQFSLWPQQCALPAGWDVVCQPQ
                     SQASCQQWLEAHWRTLTPANFTQLQEAQ"
     gene            13737..17618
                     /gene="entF"
                     /locus_tag="PN977_003321"
     CDS             13737..17618
                     /gene="entF"
                     /locus_tag="PN977_003321"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin non-ribosomal peptide synthetase
                     EntF"
                     /protein_id="HDI6043325.1"
                     /translation="MSQHLPLVAAQPGIWMAEKLSDLPSAWSVAHYVELTGEVDAPLL
                     ARAVVAGLAQADTLRMRFTEDNGEVWQWVDDAQTFELPEIIDLRTNIDPHGTARALMQ
                     ADLQQDLRVDSGKPLVFHQLIQVADNRWYWYQRYHHLLVDGFSFPAITRQIANIYCAW
                     LRGEPTPASPFTPFADVVEEYQQYRESEAWQRDAAFWAEQRRQLPPPASLSPAPLPWR
                     SASADILRLKLEFTDGEFRQLATQLSGVQRTDLALALAALWLGRLCNRMDYAAGFIFM
                     RRLGSAALTATGPVLNVLPLGIHIAAQETLPQLATRLAAQLKKMRRHQRYDAEQIVRD
                     SGRAAGEEPLFGPVLNIKVFDYQLDIPGVQAQTHTLATGPVNDLELALFPDEHGDLSI
                     EIIANKQRYAEPTLIQHAERLKMLIAQFAADPSLLCGDVDIMLPGEYAQLAQINATQV
                     EIPETTLSALVAEQAAKTPDAPALVDARYQFSYREMREQVVALANLLRERGVKPGDSV
                     AVALPRSVFLTLALHAIVEAGAAWLPLDTGYPDDRLKMMLEDARPSLLITTDDQLPRF
                     ADVPDLTRLCYNAPLTPQGSAPLQLSQPHHTAYIIFTSGSTGRPKGVMVGQTAIVNRL
                     LWMQNHYPLTGEDVVAQKTPCSFDVSVWEFFWPFIAGAKLVMAEPEAHRDPLAMQHFF
                     ADYGVTTTHFVPSMLAAFVASLTPQTARQSCATLKQVFCSGEALPADLCREWQQLTGA
                     PLHNLYGPTEAAVDVSWYPAFGEELAEVRGSSVPIGYPVWNTGLRILDAMMHPVPPGV
                     AGDLYLTGIQLAQGYLGRPDLTASRFIADPFAPGERMYRTGDVARWLDNGAVEYLGRS
                     DDQLKIRGQRIELGEIDRVMQALPDVEQAVTHACVINQAAATGGDARQLVGYLVSQSG
                     LPLDTSALQAQLRETLPPHMVPVVLLQLPQLPLSANGKLDRKALPLPELKAQAPGRAP
                     KAGSETIIAAAFSSLLGCDVQDADADFFALGGHSLLAMKLAAQLSRQFARQVTPGQVM
                     VASTVAKLATIIDGEEDSSRRMGFETILPLREGNGPTLFCFHPASGFAWQFSVLSRYL
                     DPQWSIIGIQSPRPNGPMQTAANLDEVCEAHLATLLEHQPHGPYYLLGYSLGGTLAQG
                     IAARLRARGEQVAFLGLLDTWPPETQNWQEKEANGLDPEVLAEINREREAFLAAQQGS
                     TSTELFTTIEGNYADAVRLLTTAHSVPFEGKATLFVAERTLQEGMNPERAWSPWITEL
                     DIYRQDCAHVDIISPGAFEKIGPIIRATLNR"
     gene            17833..18966
                     /gene="fepE"
                     /locus_tag="PN977_003322"
     CDS             17833..18966
                     /gene="fepE"
                     /locus_tag="PN977_003322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen length regulator"
                     /protein_id="HDI6043326.1"
                     /translation="MSSLNIKQGSDAHFPEYPLASPSNNEIDLLNLIEVLWRAKKTVM
                     AVIFAFACAGLLISFILPQKWTSAAVVTPPEPVQWQELEKTFTKLRVLDLDIKIDRTE
                     AFNLFIKKFQSVSLLEEYLRSSPYVMDQLKEAKIDELDLHRAIVALSEKMKAVDDNAS
                     KKKDEPSLYTSWTLSFTAPTSEEAQTVLSGYIDYISALVVKESIENVRNKLEIKTQFE
                     KEKLAQDRIKTKNQLDANIQRLNYSLDIANAAGIKKPVYSNGQAVKDDPDFSISLGAD
                     GIERKLEIEKAVTDVAELNGELRNRQYLVEQLTKANINDVNFTPFKYQLSPSLPVKKD
                     GPGKAIIVILSAMIGGMVACGGVLLRHAMASRKQDAVMEEHLV"
     gene            complement(18963..>19667)
                     /gene="fepC"
                     /locus_tag="PN977_003323"
     CDS             complement(18963..>19667)
                     /gene="fepC"
                     /locus_tag="PN977_003323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001601847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter ATP-binding
                     protein"
                     /protein_id="HDI6043327.1"
                     /translation="IIGPNGCGKSTLLRTLSRLMTPAHGHVWLDGEHIQHYASKEVAR
                     RIGLLAQNATTPGDITVQELVARGRYPHQPLFTRWRKEDEEAVTKAMQATGITHLADQ
                     SVDTLSGGQRQRAWIAMVLAQETAIMLLDEPTTWLDISHQIDLLELLSELNREKGYTL
                     AAVLHDLNQACRYASHLIALREGKIVAQGAPKEIVTAELIERIYGLRCMIIDDPVAGT
                     PLVVPLGRTAPSTAKI"
BASE COUNT         4542 a         4957 c         5372 g         4796 t
ORIGIN      
        1 agtacctcaa tcaggtacag gggaagctgc ctgcgggagt cagcgccgag ctggggccag
       61 acgccacggg cgttggctgg atctatgaat atgcactggt agatcgcagc ggtaagcacg
      121 atctggccga tttacgctca ttgcaggact ggtttctcaa atatgagcta aaaaccatcc
      181 ctgacgttgc ggaagtggcg tcggtgggcg gtgtggtgaa agagtatcag gtggttatcg
      241 atcctcagcg cctggcgcag tatggcatca gtctcgccga agtaaaaagc gcgctggatg
      301 cttcaaacca ggaagcgggc ggttcgtcga tcgaactggc ggaagcggaa tatatggtgc
      361 gcgccagcgg atatctgcaa acgctcgacg actttaatca catcgtttta aaagccagtg
      421 aaaatggcgt gcccgtttat ctgcgtgatg ttgcgaaggt ccaggttggc ccggagatgc
      481 gccggggcat tgccgaactg aacggcgaag gcgaagtggc gggcggggtg gtgatcctgc
      541 gttccggcaa aaacgcccgt gaagtgatcg ctgccgtgaa ggacaaactg gaaacgctga
      601 aaagcagtct gccggaaggc gtggagatag ttacaacata cgatcgcagc cagctcattg
      661 accgcgccat tgacaacctc agtggcaagt tgctggaaga gtttattgtg gtggcggtag
      721 tctgtgcgct gtttctctgg catgtgcgct cggcgctggt ggcgattatt tcgttgccgc
      781 tggggttgtg tattgctttt attgtcatgc actttcaggg actgaacgcc aatattatgt
      841 cgctgggcgg tattgcgatt gccgtcgggg cgatggtcga tgccgctatc gtcatgatcg
      901 agaatgcgca taaacggctg gaagagtggc agcaccagca tcctgacgcc acgctggata
      961 ataaaacgcg ctggcaggtg attaccgatg catctgttga agtggggccg gcgctgttta
     1021 tcagtctgct gattatcacg ctgtcgttta tcccgatctt caccctggaa gggcaggaag
     1081 gtcgtctgtt tggcccgctg gcgttcacca aaacctatgc gatggcgggc gcggcgctac
     1141 tggcgatcgt agtgatcccg atcctgatgg gctactggat ccgtggcaaa attccgccgg
     1201 aaagcagtaa cccgctcaat cgctttttga ttcgtgttta tcatccgctg ttgctgaaag
     1261 tactgcactg gccgaaaacc acgctgttgg tggcagcgct ttcggtgctg acggtactct
     1321 ggccgctcaa taaagttggc ggggaatttt taccgcagat caatgaaggc gacttgttgt
     1381 atatgccatc gacgctgccg gggatttccg cagcagaggc ggcgagcatg ctgcaaaaaa
     1441 ccgacaagct gattatgagc gtaccggaag tggcgcgggt atttggcaaa accgggaaag
     1501 cggaaaccgc caccgattca gctccactgg agatggtaga aacgactatc cagcttaagc
     1561 cacaagatca gtggcggcca ggcatgacga tggacaaaat cattgaggaa ctggataaca
     1621 ccgtgcgtct gccggggctg gcgaatctgt gggtgccgcc aattcgtaac cgtatcgata
     1681 tgctctcgac tggcattaaa agccctatcg gcattaaagt ttccggcact gtgctggcgg
     1741 atatcgacac gatggcggag caaattgaag aagtggcgcg aacggtgcca ggcgtagctt
     1801 ctgctcttgc cgagcggctg gaaggtgggc gctatatcaa cgttgagatt aaccgtgaaa
     1861 aagccgcgcg ttacggtatg acggtggcgg atgtgcaatt gtttgtgaca tctgcggtgg
     1921 gcggggcgat ggttggcgaa acggtggaag ggattgctcg ttatccaatt aatctgcgtt
     1981 atccgcaaag ctggcgcgat agcccgcagg cactgcgcca gttgccgatc ctcacgccga
     2041 tgaagcagca aatcaccctg gcagacgtgg ccgacgttaa agtctctacc ggaccgtcga
     2101 tgctgaaaac cgagaatgcg cgcccgacga gctggattta tatcgatgcc cgcgatcgtg
     2161 acatggtgtc ggtggttcac gatctgcaaa aagcgatagc tgaaaaagtg cagttaaaac
     2221 cgggcaccag cgtggcattc tccgggcagt tcgagctact ggagcgcgcc aaccataagc
     2281 tgaaactgat ggtgccgatg acgctgatga tcatcttcgt gctgttgtat ctggcgttcc
     2341 gtcgggtggg cgaagcgttg ctgattatca gcagcgtacc gtttgcgctg gtgggcggca
     2401 tctggttgct gtggtggatg ggctttcatc tttccgtggc gacgggcacc ggttttattg
     2461 cccttgccgg ggtcgccgcc gaatttggcg tggtgatgct gatgtattta cgtcacgcca
     2521 tagaggccga gccgtcgttg aataatccgc aaacattcag cgagcagaag ctggatgagg
     2581 cgttgtatca cggcgcggtc ctgcgcgtgc gcccgaaagc gatgacggtg gcggtgatta
     2641 tcgccggtct gctgccgatt ctgtggggaa cgggagcagg ttcagaggtg atgagccgga
     2701 ttgccgcgcc gatgattggc ggcatgatca ccgcgccttt gctatcgctg tttattatcc
     2761 cggcggcgta taagctgatg tggctgcacc gacatcgggt acggaaataa aagcaggata
     2821 ccccgtttaa ccgtgtggat tgtgtcttgc gacgatgggc actaaatgtt aaaaggtgcc
     2881 cctcaacaaa aaagacacac aggggaaagg cgtgaaaaac gcgtcaaccg tatcggaaga
     2941 tactgcgtcg aatcaagagc cgacgcttca tcgcggatta cataaccgtc atattcaact
     3001 gattgcgctg ggtggcgcaa ttggtactgg tctgtttctt ggcattggcc cggcgattca
     3061 gatggcgggt ccggctgtat tgctgggcta cggcgtcgcc gggatcatcg ctttcctgat
     3121 tatgcgccag ctcggcgaaa tggtggttga ggagccggta tccggttcat ttgcccactt
     3181 tgcctataaa tactggggac cgtttgcggg cttcctctct ggctggaact actgggtaat
     3241 gttcgtgctg gtgggaatgg cagagctgac cgctgcgggc atctatatgc agtactggtt
     3301 cccggatgtt ccaacgtgga tttgggctgc cgccttcttt attatcatca acgccgttaa
     3361 cctggtgaac gtgcgcttat atggcgaaac cgagttctgg tttgcgctga ttaaagtgct
     3421 ggcgatcatc ggtatgatcg gctttggcct gtggctgctg ttttctggtc acggcggcga
     3481 gaaagccagt atcgataacc tctggcgcta cggtggtttt tttgccaccg gctggaatgg
     3541 gctgattttg tcgctggcgg tgattatgtt ctccttcggc gggctggagc tgattgggat
     3601 tactgccgct gaagcgcgcg ctccggaaaa aagcatcccg aaagcagtga atcaggtggt
     3661 gtatcgcatc ctgctgtttt acatcggttc actggtggtt ttactggcac tctatccgtg
     3721 ggtggaagtg aaatctaaca gtagcccgtt tgtgatgatt ttccataatc tcgacagcaa
     3781 cgtggtagct tctgcgctga acttcgtcat tctggtagca tcattatcgg tgtataacag
     3841 cggggtttac tctaacagcc gcatgctgtt tggcctttct gtgcagggta atgcgccgaa
     3901 gtttttgact cgcgtcagcc gtcgcggtgt gccgattaac tcgctgatgc tttccggagc
     3961 gatcacttcg ctggtggtgt taatcaacta tctgctgccg caaaaagcgt ttggtctgct
     4021 gatggcgctg gtggtagcaa cgctgctgtt gaactggatt atgatctgtc tggcacatct
     4081 gcgttttcgt gcagcgatgc gacgtcaggg gcgtgaaaca cagtttaagg cgctgctcta
     4141 tccgttcggc aactatctct gcatcgcctt cctcggcatg attttgctgc tgatgtgcac
     4201 gatggatgat atgcgcttgt cagcgattct gctgccggtg tggattgtat tcctgtttgt
     4261 ggcatttaaa acgctgcgtc ggaaataagg cattcacgcc acatccgaca aaacgatgtc
     4321 cgctctcatc catgcgatga gagcggtttt tttaattact gcttaaatgc acccgccaga
     4381 gagcgaatat cattgccggt tggcgactga tgaagtcgca gaccaaactc ttcgacaatc
     4441 gcaaatatgt ggtcgaatat atcagcctga atgctttcat attccagcca caccacggtg
     4501 ttggtaaacg catagatctc gagcggtaaa ccgttatcgc caggagccag ttggcgtacc
     4561 attaaggtca tatctttacg aatccgcgga tggttacgta gatattcgtt cagataggca
     4621 cgaaaggttc caatattggt cattcggcgc aggtttaata ccgactccgt agaaccttgc
     4681 tggcgattcc actcattaat ttccttgtgg cggctggtta aataaggttt taacaaatgc
     4741 gctttattca gacgttgcat ttcgtcttca tcaagaaaac gaatactggt gacatcaata
     4801 ctgatactgc gcttaatacg tcgcccgcca gatgctgaca tcccgctcca gtttttaaag
     4861 gagtcagaga ccagagacca ggtgggaata gtggtaatgg tattgtccca gttacgcact
     4921 ttgacggtgg ttaacccaat atcgatcacc gcgccatccg cgccgtattt cggcatctcc
     4981 agccagtcgc ccagtttcag catatcgttc gcggaaagct gaatacctgc caccagacca
     5041 agaatcggat ctttaaatac caacatcagc acggcagcca ttgcaccaag accgctgatc
     5101 agaatcgctg gcgactgacc aatcagcagt gagatcatca aaatgccgac cagaatcgcg
     5161 ccgatcagtt taatcccctg aaatatccct ttcagcggta actgagatgc cgccgggaat
     5221 ttctgtgcca gattcaaaat aacatccagc agagagaaaa ctgaaagcag cgcatacatc
     5281 atgatccaca actgcgcgca ggtagtcaga atatctgccg cttcggtgcc tttttgcagc
     5341 cagaataccg cctgaatatt gacgataatc ccctgcaggg taaaagctaa acggtggaag
     5401 agtttattct gggtaatgat ttgcaaccat agccgtgaac tggcgatggc acgtttttcg
     5461 aaggtccgca gtaccaccca atgcaaaaaa atatgcacca cgacggcggt cagaaaaata
     5521 ataccgaaaa tcatcaccat tgaggtggtg tgatcgatct caatacccgc taaatcttca
     5581 acctgggata ttaaatcctg cataacgtct cctttataca acagcagact atgatgacgg
     5641 ctgaaacagg gttatgcaaa tcaggagaat ctgaggggaa atagccgggc agatgcccgg
     5701 caagagagaa ttacacttcg gttaaggtga tgttttgcgg cagacgagat ttcggcagcg
     5761 tagcgttaaa atcttcaacg ctgtgatgac ccaccggaac aactaccaga ctggtgtagc
     5821 ctttctcttt cagaccaaat tctgcatcga ggatggcggc gtcaaaacct tcgatgggta
     5881 ccgcgtccag acccagcgcc gccacgccga gcaggaagtt gccgacgttg agataaacct
     5941 gttttgccat ccactctgca tcatcatgca gatctttacg gtgcatatcg gcgaagaact
     6001 tgcgaccttt atcgttcgcg gctttcgctt ccggcgtggc aaagcggcca tcggcatctt
     6061 cctggtcaac aaccagcttc agccaggcat cgtccatcgc ggtttttgca cagaacacca
     6121 cgacgtgcga ggcatcaagc attttacgct cgttgaacac gtaattaccg gcagcggatt
     6181 tggcaacacg cgctttacct tcttccgtgc tggcaacaat aaaatgccac ggctgggagt
     6241 tggtgctgga tgggctgtat tgcagtagag tttttatctg ctcggcctgt tccggggtaa
     6301 gttttttgct ggcatcaaat gccttagtgg aatgacgctt taaggcgaca gaaatgatat
     6361 ccataaagac tccatgtgaa agtaattttg cgtgccagca gattacaagg atcgagatga
     6421 aatggtaagc gagaaaaatg cgctattgat ttacggttga gtcaggacgc actctttttt
     6481 gatcgcgttt agccagacca tcaaccacca gattccacga atcgttgatc agatcgcgaa
     6541 gtaacgcttc ggagatttcc tcgccgggat acaccgaaat ccagtgcttt ttattcatgt
     6601 gataccctgg cttgatgctt gggtatattt gctgatttag cagggatttt tgtggatcgg
     6661 acttcagatt gataaagggg acgccgcgca gctccgacga cagcataaaa atcttgccgc
     6721 caattttaaa aacatcgaac tccgggccaa aaggccagca aagctcgaca aagggtaact
     6781 caagagccag gcgtttcgcc gtttcgtgca gtgattgctt atccataaac gttcctttag
     6841 gcgaaggaga ataagcaaag tatgccgcga agttcggcga taatcgacgt ttaatccgcc
     6901 agcgagaacc agcgtcgcca gataaagcgc agaacaaaat actcaatagc gcccagcact
     6961 aaaaaccaca gacaaaacaa taaagtgtaa agctggctaa gatccatcag atggaacgtg
     7021 gtcaccagtt tttgtgccag cgtcagcccc aatgcggggg cgggaagcag caggcaagag
     7081 aggaaagcca tcagcaaaat gcctgctgcg gtggtcaagg tttctaaagg gtgtttcata
     7141 acattgttaa atgtaagtta aaacaccatt gtcagggata ttcttctgta aggcaattcc
     7201 cggcttagtc accggcccag atctcacaat gctttttcac cagcccaatc agcgagccgc
     7261 catcggcgac gaaatcgcgc atcagctgcg cttcattcag accgctgacg acctggcgat
     7321 gcagggcttc aattgcgctc gatgcgccaa ttttatttgc agaaggggcg attttttcca
     7381 gcaatcgcaa ggtgtcttcc gttagcggtc gacgatcgcc agtgtgcgga tcggtaatga
     7441 cacctgccag cccatagcgg caggcctgga aacggttgaa tttatacagc aggtaatctt
     7501 tctcctgatg tttaaacggg cgttccgtca gtaaccagtg ggcggtagcc tgaatcaatc
     7561 ctgccatatt taccgcatgg ctaagggtta acggggtatc cattacccga acctccaccg
     7621 tgccaaaatg aggactgggg cgaatatccc agtgcagatc tttaatgctg tcgatcatcg
     7681 tggtgtaact caggcagcga aacagggctt caaattgttg ccagttactg acccacggca
     7741 tcgggccatt atcgggaaag gcggaaaaaa tattcggtcg tgaggaggca aaacgcgtat
     7801 ccgttccttg catatatggc gacgcggcgg aaagggcgat aaagtgcggc acaaaccgtg
     7861 acaggccgtg cagcaaataa atggcatcat cgccactggc gcaaccaaca tggacatgct
     7921 gaccaaaaac ggtcgcctgt tgaatgagat aaccaaagtt ttccagcgtt cgttgatagc
     7981 gttcgttatc gcatacctcc tgacgctgcc atttctgaaa cgggtgcgtg ccaccgccgc
     8041 aaatttccag atgatgatct gcggctgcct gcaatacgac tttctgcatc gctgaaaatt
     8101 gcccggcagc ctggttgata tcacggcaaa catccgtcgc cagctccagc atactttcgg
     8161 tgatatcgtg ctttacctct ccggccgtga tcttattttt aactgcgtca atcagcattg
     8221 aagagtcctg gcttaagtca tagcccggcg gattaaccac ctgcatttcc agttcaatac
     8281 cgagggtaaa aggttcagaa acatgaaaat cgggtaatgg cataggtttc tcttatgttg
     8341 gcgttttcta ttcagtatag aagtgggagc gactgggcga gatgcggaag ttctggaata
     8401 tttctttttt tggtgatggt gactgaagca aattggctac attttgcaat gtgaccagtt
     8461 gcgtcatagt tggctggcgg cgaagaattt tgacgattga ggcgtgcaga taaaaacggg
     8521 ttcagcttca gttgatcaaa tggggagtga gaagtccgaa acaggattta ctgtataaat
     8581 aaacagctat tttgttgagg aagggtaaga taacggcggg tgcctgaagc tttccggttt
     8641 caggttaact ttgaggtctg gaaagatgaa gccccaggag atatttctat caaccctggg
     8701 gctgccactc caaacccgaa caatttggat ggtagtccct tcttcgcatg gaggcaatat
     8761 aaacatgctg acgaaatatg cccttgtggc agtcatagtg ctgtgtttga cggtgctggg
     8821 atttacgctt ctggtcggag actcgctgtg tgagtttacg gtgaaggaac gtaatattga
     8881 gtttaaggct gttctcgctt acgaaccgaa gaagtagccg ttgtgcgggg agcaatcccc
     8941 gcatatccgg ttgtcaggtc aggatggtaa ggcacctgct ttacactttc gcccgtggtc
     9001 agtgatggct gcgggcgaat cgtaccagat attgtcaatt aattgtgttg gcacagcgtt
     9061 atgactatct tttcttttat ctgccagtgc acagcaaaca tcttattctc acgatgaatg
     9121 atgacctgct gtttattcca gttaattatc tgatagtcca gaaatcctgc atcagtttgt
     9181 ctctcacatg ccttaaatgc gctctctttg gcggaaaatg ccagtgtcag cgccagagaa
     9241 aaggctaaac cgcagtctgc gagtcgcttg tgttccgctg gcgtaataat gttgtctgtc
     9301 aattctgccg ctgtttgcgc agaaaaaatc tcttcaatat caatgccaat cggttgacga
     9361 gataccacgg ctaatgccgt agtcccacag tgactaatgc tgccgtatac ccccgcaggc
     9421 cacacaggtt ggcgtagctc gccgattgcg ggcacacatt tatagccata ttcccgcagc
     9481 gcataaactg cagcgatccg tccggctaaa tgctctgttt tacgtttacg tccagcgtgt
     9541 tgcagttgcg cgtagtgtgg cagccagagt aaatcctgct cacaaaaact cgccggatcg
     9601 aacttaacaa aatgcagcgt atttccggca aaggggaggg cggtatgcgt agttttcatg
     9661 tcgaccatat tagagactga tgacaaacgc aaaactgcct gatgcgctac gcttatcagg
     9721 cctacactat cctgcaatat attgaattgg caagattttt gtaggccgga taaggcgttc
     9781 acgccgcatc cggcatgaac gacgcgcact ttgtcaacaa tctgacgtta gcatcagaag
     9841 tgagtgttta cgctcatata ccacgtacgt cccggctcgt tataggtata cgcaccggca
     9901 ccggcgatat agttggcccc tgccagatcg cccgtggtct gggcattacc cgcacgccac
     9961 aaacgtttgt cgaacaggtt gtccacgccg ccggtcagac tgacattctt cgtcacatcc
    10021 caggtcgcgc tcaggccaac aatgctgtac gggctgattt ctttggtttc cggtccaacc
    10081 gctggctgac ctttatagtt gtacttcttc ggctgctgct tgccgtacca ggtgaaggtt
    10141 gtttgcatcg acaaatcttc ccgtgcctgc cagctcagcg ttgagttcaa cgtatactcc
    10201 gggatgatcg acaaacggtc gcccgtggtt ttgttttcac tcttcagcat ataagtgatg
    10261 ttattggtcc acatcaccgt ttcgctaacc ggtacgttta acgatccttc cagaccttca
    10321 accaccgctt tcggcacgtt atcccactga tagagatcgg tgccagccgc attttgccct
    10381 acagccacat agcccgcttc aatcttattg cgataatcgt tacggaacca ggtcacgccc
    10441 gccagccagc cgtcgcgttt gaactccaga ccaatctctt tgttgatact ggtttccgct
    10501 ttcaggtcat cgttcccctg cagatagcag ccgcccgcgc tggcgtaaca gccctgacct
    10561 ttactgtaga gaatgtagtt cgggttagtc tggtacaggc tcggcgcttt ataggcgcgg
    10621 gcaatgccca ttttcagcgt gaagtcatca cctaaacctt gcgatatgtt tagagccgga
    10681 ctccagttat tgccaacaat gctgtgatga tcgaaacgca gccccggcgt tacgatggtg
    10741 ctgtcagtca gctccatatt gttttcggca aatagcgaga aaatttctgc ttttgaatac
    10801 gggctacggt cggtggcact cacaccatca atagcgccgc cggtattcgt cccggtgagc
    10861 gcctgggtgt tggaacttaa atccttcatc cgctgctgat tccactctgt acccagcgtc
    10921 agcgtttggt ttacgaggaa atcaatcggc aggttaactt cgctgtgcag catcacgtca
    10981 tcaagatcga tatctacaaa atcctgtgcc gctttttcgt taaatttccc ttcagtaccg
    11041 cccgccagac cttccggaat acgcgagtta cgggtgtgtt cgtactgcac ccagttgctg
    11101 gtggtcacgc cgttatccca gccaccgttc caggtcagcg agtagttctg gcgatacagg
    11161 cggttggttt catcgccata tttcgagcga gtgtaagcgt cagagttggt gttctgcgta
    11221 tcacccgcat acaggttacc ctggcggctg tagcccgctt ccagttcgag tgactgcaga
    11281 ggcgcgaagt cccagcgcac cacgccatta atatctttgt tgatcacccc ttcgcgcccg
    11341 gctggtaacg tcgtggcata cgttccggca cgcgcggact gatggccctg gttgatatcc
    11401 cacgcgtcag cctgggtttt gtcgaggttg ccatacaagc ggaagctgaa ttcgtcgccc
    11461 agcggaccgg tcaggctgaa gttggtgcgt ttggtggcac cttcctcttt atgttctggc
    11521 gcattgaaat aagcatccca ggagccgtgc cactcaccgc tgcctttttt ggtaatgatg
    11581 ttaaccacgc cgcccgccgc gccgttgcca taacgcgcag ctgccggacc acgcagaact
    11641 tcaatacgtt caatcatttc aggcggcacc caggaggtat caccacgggt atcgcgctcg
    11701 ccacgccagc cctgacgcac tgagttacgg ctgcttactg gcttgccgtc aatcaaaatc
    11761 agcgtgtttt ccggacccat gccgcgaata tcaatctggc ggttattacc acgctgacca
    11821 ctggtggaat taccggtcag gttaacgcca ggcatggtac ggataatctc cgatacatcg
    11881 cgggcaaccg ggtttttgcg gatttcatct gcggtgatgg tcgaaacgcc gggcgcctgc
    11941 aagttctgct cggcggcggt aacaacaata gtatcgtcat gtgaaacagg agtatcggtc
    12001 ggctcttgtg cctgtgctac cccataaatc cccagattga ccaacaaggc cagggaatga
    12061 atcttcttgt tcattgtttt attcctgcat ttttgccacg aattgcaact gtcgggcatg
    12121 gtcgtcatca acacgacgca tcccgctacc gcgaaaacct ttgatcctga aagacacgca
    12181 gtgcagctga ttaattaatg tccgcgcttc ccacggcgcg ccattacgct attgcaaatg
    12241 caaatagtta tcaataatat tatcaatata tttctgcaat caataaaaaa ttgcacagta
    12301 aacatggggt tatggtgtga cggcgttaaa agtaggaagt gagagctggt ggcagtcgaa
    12361 acatggcccg gaatggcaac gtctgaatga cgaaatgttt gaggtcactt tctggtggcg
    12421 tgatccacaa ggttctgaag gccactcgac gataaagcgc gtatgggtct atatcactgg
    12481 catgaccgat caccatcaga acagccagcc ccggtcgatg cagcgaattg caggaaccga
    12541 tgtttggcag tggacgacac aactcaatgc caactggcgc ggcagctact gctttattcc
    12601 taccgaacgc gatgacattt tttctgcacc atcccccgat cgcctcgaat tacgcgaagg
    12661 ctggcgaaaa ctattacccc aggcgatagc cgatccgctg aatccacaaa gctggaaagg
    12721 cgggcgaggg cacgctgttt ctgcactcga aatgccgcaa gcgcctctgc aaccgggatg
    12781 ggattgtccg caagcgccag aaacgcctgc caaagaaatt atctggaaaa gtgaacggtt
    12841 gaaaaattca cgccgtgtat ggatttttac caccggcgat gcaacagcag aagaacgccc
    12901 gctggcagtt ttgcttgatg gcgaattttg ggcgcagagt atgcccgtct ggcctgcgct
    12961 gacttcgctg acccatcgtc ggcaacttcc gcccgccgtg tatgtgttga tcgacgctat
    13021 cgacaccacg caccgcgccc acgaactgcc gtgtaatgcg gatttctggc tggcagtaca
    13081 gcaagagtta ttaccccagg tgaaagctat tgcccctttt agcgatcgcg ccgatcgcac
    13141 cgtggtcgcc ggacagagtt ttggtgggct ttccgcgctg tatgccggac tgcactggcc
    13201 tgaacgcttt ggctgtgtat taagccagtc aggctcgtac tggtggccgc atcggggcgg
    13261 gcaccaagag ggcatgttac ttgaacagct aaatactggt gaagttagcg ccgaaggtct
    13321 gcgcattgtg ctggaagcgg gtgttcgcga gccgatgatc atgcaggcca atcaggcgct
    13381 gtatgcgcaa ttacaccccc taaaagaatc cattttctgg cgtcaggttg acggcggaca
    13441 tgatgcgctt tgttggcgcg gtggcttgat gcaggggcta atcgacctct ggcaaccact
    13501 tttccatgac aggagttgaa tatggcattc agtaatccct tcgatgatcc acagggagcg
    13561 ttttacattt tgcgcaatgc gcaggggcaa ttcagtctgt ggccgcaaca atgcgcctta
    13621 ccggcaggct gggacgttgt gtgtcagccg cagtcacagg cgtcctgcca gcagtggctg
    13681 gaagcccact ggcgtactct gacaccggcg aatttcaccc agttgcagga ggcacaatga
    13741 gccagcattt acctttggtc gccgcacagc ccggcatctg gatggcagaa aaactgtcgg
    13801 atttaccctc cgcctggagc gtggcgcatt acgttgagtt aaccggagag gttgatgcgc
    13861 cactactggc ccgcgcggtg gttgccggac tagcgcaagc agatacgttg cggatgcgtt
    13921 ttacggaaga taacggcgaa gtctggcagt gggtcgatga tgcgcagacg ttcgaactgc
    13981 cagaaattat cgacctgcga accaacattg atccgcacgg tactgcgcgg gcattaatgc
    14041 aggcggattt gcaacaagat ctgcgcgtcg atagtggtaa accactggtc tttcatcagc
    14101 tgatacaggt ggcggataac cgctggtact ggtatcagcg ttatcaccat ttgctggtcg
    14161 atggcttcag tttcccggcc attacacgcc agatcgccaa tatttactgc gcatggctgc
    14221 gtggcgaacc aacgcctgct tcgccgttta cgcctttcgc tgatgtagtg gaagagtatc
    14281 agcagtaccg cgaaagtgaa gcctggcagc gtgatgcggc attctgggcg gaacagcgtc
    14341 gtcaactgcc accgcccgcg tcactttctc cggcaccttt accgtggcgc agcgcttcgg
    14401 cagatatttt gcgcctgaaa ctggaattta ccgacgggga attccgccag ctggctacgc
    14461 aactttcagg tgtgcagcgt accgatttag cccttgcgtt ggcagctttg tggctggggc
    14521 gattgtgcaa ccgcatggac tacgctgccg gatttatctt tatgcgtcga ctgggctcgg
    14581 cggcgctgac ggctaccgga cccgtgctca acgttttgcc gttgggtatt cacattgcgg
    14641 cgcaagaaac gctgccgcaa ctggcaaccc gactggcggc tcaactgaaa aaaatgcgcc
    14701 gccatcaacg ctacgacgcc gaacaaattg tccgtgacag tgggcgagcg gcaggagagg
    14761 aaccgctgtt cggtccggta ctcaatatca aggtatttga ttaccaactg gatattcctg
    14821 gtgttcaggc gcaaacccat accctggcaa ccggtccggt taatgacctt gagctggccc
    14881 tgttcccgga tgaacacggt gatttgagta ttgagatcat cgccaataaa cagcgttacg
    14941 ctgagccaac gttaatccag catgctgaac gcctgaaaat gctgattgcg cagttcgccg
    15001 cggatccgtc gctgttgtgc ggcgatgtcg atattatgct gccaggcgag tatgcgcagc
    15061 tggcgcagat caacgccact caggttgaga ttccagaaac cacgctgagc gcgctggtgg
    15121 cagaacaagc ggcaaaaaca ccggatgctc cggcgctggt tgatgcgcgt taccagttca
    15181 gctatcggga aatgcgcgaa caggtggtgg cgctggcgaa tctgctgcgt gagcgcggcg
    15241 ttaaaccagg cgacagcgtg gcggtggcat taccgcgctc ggtctttttg accctggcgc
    15301 tacatgcgat tgttgaagct ggtgcggcct ggctaccgtt ggataccggc tatccggacg
    15361 atcgcctgaa aatgatgctg gaagatgcgc gtccgtcgct gttaatcacc accgacgatc
    15421 aactgccgcg ctttgccgat gttccagatt taacccgcct ttgctataac gccccgctta
    15481 caccacaggg cagtgcgccg ctgcagcttt cacaaccgca ccacacggct tatatcatct
    15541 tcacctccgg ttccaccggc agaccaaaag gggtaatggt cgggcagacg gctatcgtta
    15601 atcgcctgct ttggatgcaa aaccattacc cgcttacagg cgaagatgtc gttgcccaaa
    15661 aaacgccatg cagttttgat gtctcggtgt gggagttttt ctggccgttt attgccgggg
    15721 cgaaacttgt gatggctgaa ccggaagcgc accgcgaccc gctcgctatg cagcattttt
    15781 ttgctgatta tggcgtaacg accacgcact ttgtgccgtc gatgctggcg gcatttgttg
    15841 catcgctgac gccgcaaacc gctcgccaga gttgcgcgac gttgaaacag gttttctgta
    15901 gtggtgaagc cttaccggct gatttatgcc gcgaatggca acagttaacc ggcgcgccgt
    15961 tgcataatct atatggcccg acggaagcgg cggtagatgt cagttggtat ccggcttttg
    16021 gcgaggaact ggcagaggtg cgcggcagca gtgtgccgat tggttatccg gtgtggaata
    16081 cgggcctgcg cattctcgat gcgatgatgc atccggtgcc gccgggtgtg gcgggagatc
    16141 tctatctcac cggtattcaa ctggcgcagg ggtatcttgg acgacccgat ctgaccgcca
    16201 gccgctttat tgccgatcct tttgccccag gtgaacggat gtaccgtacc ggcgatgtgg
    16261 cccgctggct ggataacggc gcagtggagt acctcgggcg cagtgacgat cagctaaaaa
    16321 ttcgcgggca gcgtatcgaa ctgggcgaaa ttgatcgcgt gatgcaggcg ctgccggatg
    16381 tcgaacaagc cgttacccac gcctgtgtga ttaaccaggc ggcagccact ggtggtgatg
    16441 cgcgtcagtt ggtgggctat ctggtgtcgc aatcaggtct gccgttggat accagcgcat
    16501 tacaggcgca gcttcgtgaa acattgccac cacatatggt accggtggtt ctgctgcaac
    16561 ttccacagtt accacttagc gccaacggca agctggatcg caaagcctta ccgttgcctg
    16621 aactgaaggc acaagcgcct gggcgtgcgc cgaaagcggg cagtgaaacg attatcgccg
    16681 cggcattctc gtcgttgctg ggttgtgacg tgcaggatgc cgatgctgat ttctttgcgc
    16741 ttggcggtca ttcgctactg gcaatgaaac tggctgcgca gttaagtcgg cagtttgccc
    16801 gccaggtgac gccggggcaa gtgatggtcg cgtcaactgt cgccaaactg gcaacgatta
    16861 ttgatggtga agaagacagc tcccggcgca tgggattcga aaccattctg ccgttgcgtg
    16921 aaggtaatgg cccgacgctg ttttgtttcc atccggcctc cggttttgcc tggcagttca
    16981 gcgtgctctc gcgttatctc gatccacaat ggtcgattat cggcattcag tcgccgcgcc
    17041 ccaatggccc catgcagacc gcggcaaatc tggatgaagt ctgcgaagcg catctggcaa
    17101 cgttacttga gcatcaaccg cacggccctt attacctgct gggttattcg ctgggcggta
    17161 cgctggcgca gggtattgcg gcgcggctgc gtgcccgtgg cgaacaggtg gcatttcttg
    17221 gcttgctgga tacctggccg ccagaaacgc aaaactggca ggaaaaagag gctaatggcc
    17281 tggacccgga agtgctggcg gagattaacc gcgagcgcga ggccttcctg gcggcacagc
    17341 agggaagtac ttcaacggag ttgtttacca ccattgaagg caactacgct gatgctgtgc
    17401 gcctgctgac gactgctcat agcgtaccgt ttgaaggtaa agcgacgctg tttgttgctg
    17461 aacgcacgct tcaggaaggt atgaatcccg aacgcgcctg gtcgccgtgg attacggagc
    17521 tggatatcta tcgtcaggat tgtgcgcatg tggatattat ctctccaggg gcgtttgaaa
    17581 aaattgggcc gattattcgc gctacgctaa acaggtaaat taatattatc tataaaccca
    17641 taattacagg aaataattat gggtttatca tttatttaat ttataagttt ggtggatatt
    17701 tctcttaaat agagtgaatg ttgcattcag tggaaggaat ttacaaactt cagcttcgga
    17761 taattctcac caactggtaa agtgagcgcc gtggagattt actcttattg gacccatttc
    17821 aggttatacc ccatgtcatc actgaatatt aaacagggaa gtgacgctca ttttcccgag
    17881 tatcctctgg cgtcgcccag taataatgaa attgatttac ttaatctaat cgaagtttta
    17941 tggcgggcaa aaaaaacggt catggcggtc atttttgcgt ttgcctgcgc aggattgctg
    18001 atctctttca tcctgccgca aaaatggacc agcgcggcgg ttgtcacgcc tccagaacct
    18061 gttcagtggc aagaactgga gaaaacattc actaagcttc gtgtgctgga tctggatatc
    18121 aaaattgatc gtacagaagc atttaacctg tttatcaaga agtttcagtc ggttagcttg
    18181 ctggaagagt acctgcgttc atcaccttat gtgatggacc aattaaaaga ggcgaaaatc
    18241 gacgaactgg atttgcatcg agctatcgtc gcattgagcg aaaaaatgaa agcggttgat
    18301 gacaatgcca gtaagaaaaa agatgaaccg tcactgtata cctcctggac gctaagtttt
    18361 accgcgccaa ccagtgaaga ggcgcagacc gttttgagcg ggtatatcga ttatatctct
    18421 gcgttggtgg tgaaagagtc gatagaaaac gtccgtaata aactggagat caaaacccag
    18481 tttgaaaaag aaaaactggc tcaggatcgc attaaaacga aaaatcaact tgatgcaaac
    18541 attcagcgcc tcaattattc actcgacatt gccaacgcgg caggaattaa aaagcccgtt
    18601 tacagtaatg gtcaggccgt taaagatgac cccgatttct ctatttctct cggcgcagac
    18661 ggtattgaac gcaaactgga aattgaaaaa gcggttactg acgttgcgga attgaacggt
    18721 gaattacgta atcgtcagta tcttgttgag cagctaacaa aagcaaatat caacgatgtg
    18781 aattttacgc cgtttaaata tcagttaagc ccgtcattgc cagtgaaaaa agacggtccg
    18841 ggtaaggcga ttattgtgat cctttctgcg atgatcggcg ggatggtggc ttgtggtggc
    18901 gtgctgttgc gccatgcgat ggcatccaga aaacaggatg ccgtgatgga agagcactta
    18961 gtttaaatct tggcagttga aggtgccgtt cgtccgagcg gcaccacgag cggcgttccg
    19021 gcaaccggat cgtcaataat catgcagcgc agaccataaa tgcgctcaat cagttcagca
    19081 gtgacaatct ccttcggcgc tccctgagca acaattttcc cttcccgcaa tgcaatcaaa
    19141 tggctggcgt aacgacaggc ctgattaaga tcgtgcagca ccgccgccag ggtatagcct
    19201 ttctcgcggt tcagttcgct taacagttcc aggagatcaa tctgatgact gatatccagc
    19261 caggtcgtgg gttcgtcgag cagcataatt gccgtttcct gggccagcac cattgctatc
    19321 cacgctcgct ggcgttgtcc gccagaaagg gtatccacgc tttgatctgc cagatgagtg
    19381 attcccgtgg cctgcatcgc ttttgtgact gcctcttcat cttctttccg ccagcgggta
    19441 aacagcggtt gatgcggata acgcccacgc gccaccagct cctgcacggt gatatcgccc
    19501 ggcgtggtag cattttgcgc caacagacca atccgccggg caacttcttt actggcgtaa
    19561 tgttgaatgt gctcgccatc cagccagaca tgcccgtgag caggcgtcat caggcggctt
    19621 aaagtacgca gtaaagtgga tttaccgcag ccatttggcc cgataat
//
LOCUS       DAMZFT010000089        19486 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.089, whole genome shotgun sequence.
ACCESSION   DAMZFT010000089 DAMZFT010000000
VERSION     DAMZFT010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19486)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19486)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19486
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.089"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..834
                     /locus_tag="PN977_003324"
     CDS             <1..834
                     /locus_tag="PN977_003324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDI6043328.1"
                     /translation="GNQQLGYIFAATLFSLFGLLFMWICYSGVKERYVETQPTNPAQK
                     PGLLQSFRAIAGNRPLFILCIANLCTLGAFNVKLAIQVYYTQYVLNDPILLSYMGFFS
                     MGCIFIGVFLMPGAVRRFGKKKVYIGGLLIWVLGDLLNYFFGGGSVSFVAFSCLAFFG
                     SAFVNSLNWALVSDTVEYGEWRTGVRSEGTVYTGFTFFRKVSQALAGFFPGWMLTQIG
                     YVPNVAQADHTIEGLRQLIFIYPSALAVVTIVAMGCFYSLNEKMYVRIVEEIEARKRT
                     A"
     gene            877..2280
                     /locus_tag="PN977_003325"
     CDS             877..2280
                     /locus_tag="PN977_003325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDI6043329.1"
                     /translation="MSDHNPLTLRLNLREKIAYGMGDVGSNLMLCIGTLYLLKFYTDE
                     LGMPAYYGGIIFLVAKFFTAFTDMLTGFLLDSRKNIGPKGKFRPFILYAAVPAALIAT
                     LQFIATTFSLPVKTTIATALFMMFGLSYSLMNCSYGAMIPAITKNPNERAQLAAYRQG
                     GATIGLLICTVAFIPLQSLFSDSTVGYACAALMFSIGGFIFMMLCYRGVKEHYVDTTP
                     TGHKASILKSFCAIFHNPPLLVLCIANLCTLAAFNIKLAIQVYYTQYVLNDINLLSWM
                     GFFSMGCILVGVLLVPLTVKCFGKKQVYLAGMVLWAVGDILNYFWGSNSFTFVMFSCV
                     AFFGTAFVNSLNWALVPDTVDYGEWKTGIRAEGSVYTGYTFFRKISAALAGFLPGIML
                     TQIGYVPNIAQSDATLQGLRQLIFIWPCALAMIAALTMGFFYTLNEKRFALIIEEINQ
                     RKNKEIETEEKTASVTL"
     gene            2347..3039
                     /gene="ompL"
                     /locus_tag="PN977_003326"
     CDS             2347..3039
                     /gene="ompL"
                     /locus_tag="PN977_003326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpL"
                     /protein_id="HDI6043330.1"
                     /translation="MKKINAIILLSSLTSASVFAGAYVENREAYNLASDQGEVMLRVG
                     YNFDMGAGIMLTNTYTFQREDELKHGYNEIEGWYPLFKPTDKLTIQPGGLINDKSIGS
                     GGAVYLDVNYKFTPWFNLTVRNRFNHNNYSSTDLNGDLDNNDSYEIGNYWNFIITDKF
                     SYTFEPHYFYNINDFNSSNGTKHHWEITNTFRYRINEHWLPYIELRWLDRNVEPYHRE
                     QNQIRIGTKYFF"
     gene            complement(3131..4396)
                     /locus_tag="PN977_003327"
     CDS             complement(3131..4396)
                     /locus_tag="PN977_003327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDI6043331.1"
                     /translation="MLTKKKWALFSLLTLCGGTIYKLPSLKDAFYIPMQEYFHLTNGQ
                     IGNAMSVNSFVTTVGFFLSIYFADKLPRRYTMSFSLIATGLLGVYLTTMPGYWGILFV
                     WALFGVTCDMMNWPVLLKSVSRLGNSEQQGRLFGFFETGRGIVDTVVAFSALAVFTWF
                     GSGLLGFKAGIWFYSLIVIAVGIIIFFVLNDKEEAPSVEVKKEDGASKNTSMTSVLKD
                     KTIWLIAFNVFFVYAVYCGLTFFIPFLKNIYLLPVALVGAYGIINQYCLKMIGGPIGG
                     MISDKILKSPSKYLCYTFIISTAALVLLIMLPHESMPVYLGMACTLGFGAIVFTQRAV
                     FFAPIGEAKIAENKTGAAMALGSFIGYAPAMFCFSLYGYILDLNPGIIGYKIVFGIMA
                     CFAFCGAVVSVMLVKRISQRKKEMLAAEA"
     gene            complement(4498..5478)
                     /locus_tag="PN977_003328"
     CDS             complement(4498..5478)
                     /locus_tag="PN977_003328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HDI6043332.1"
                     /translation="MVTINNARKILQRVDTLPLYLHAYAFHLNMRLERVLPADLLDIA
                     SENNLRGVKIHVLDGERFSLGNMDDKELSAFGDKARRLNLDIHIETSASDKKSIDEAV
                     AIALKTGASSVRFYPRYEGNLRDVLSIIANDIAYVRETYQDSGLTFTIEQHEDLKSHE
                     LVSLVKESEMESLSLLFDFANMINANEHPIDALKTMAPHITQVHIKDALIVKEEGGLG
                     HKACISGQGDMPFKALLTHLICLGDDEPQVTAYGLEEEVDYYAPAFRFEDEDDNPWIP
                     YRQMSETPLPENHLLDARLRKEKEDAINQINHVRNVLQQIKQEANHLLNH"
     gene            complement(5486..6196)
                     /locus_tag="PN977_003329"
     CDS             complement(5486..6196)
                     /locus_tag="PN977_003329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTRA domain-containing protein"
                     /protein_id="HDI6043333.1"
                     /translation="MAENQSTVENAKEKLDRWLKDGITTPGGKLPSERELGELLGIKR
                     MTLRQALLNLEAESKIFRKDRKGWFVTQPRFNYSPELSASFQRAAIEQGREPSWGFTE
                     KNRTSDIPETLAPLIAVTPSTELYRITGWGALEGHKVFYHETYINPEVAPGFIEQLEN
                     HSFSAVWEKCYQKETVVKKLIFKPVRMPGDISKYLGGSAGMPAILIEKHRADQQGNIV
                     QIDIEYWRFEAVDLIINL"
     gene            complement(6413..8236)
                     /gene="typA"
                     /locus_tag="PN977_003330"
     CDS             complement(6413..8236)
                     /gene="typA"
                     /locus_tag="PN977_003330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-dependent GTPase TypA"
                     /protein_id="HDI6043334.1"
                     /translation="MIEKLRNIAIIAHVDHGKTTLVDKLLQQSGTFDSRAETQERVMD
                     SNDLEKERGITILAKNTAIKWNDYRINIVDTPGHADFGGEVERVMSMVDSVLLVVDAF
                     DGPMPQTRFVTKKAFAYGLKPIVVINKVDRPGARPDWVVDQVFDLFVNLDATDEQLDF
                     PIVYASALNGIAGLDHEDMAEDMTPLYQAIVDHVPAPDVDLDGPFQMQISQLDYNSYV
                     GVIGIGRIKRGKVKPNQQVTIIDSEGKTRNAKVGKVLGHLGLERIETDLAEAGDIVAI
                     TGLGELNISDTVCDTQNVEALPALSVDEPTVSMFFCVNTSPFCGKEGKFVTSRQILDR
                     LNKELVHNVALRVEETEDADAFRVSGRGELHLSVLIENMRREGFELAVSRPKVIFREI
                     DGRKQEPYENVTLDVEEQHQGSVMQALGERKGDLKNMNPDGKGRVRLDYVIPSRGLIG
                     FRSEFMTMTSGTGLLYSTFSHYDDVRPGEVGQRQNGVLISNGQGKAVAFALFGLQDRG
                     KLFLGHGAEVYEGQIIGIHSRSNDLTVNCLTGKKLTNMRASGTDEAVVLVPPIRMTLE
                     QALEFIDDDELVEVTPTSIRIRKRHLTENDRRRANRAPKDD"
     gene            8609..10018
                     /gene="glnA"
                     /locus_tag="PN977_003331"
     CDS             8609..10018
                     /gene="glnA"
                     /locus_tag="PN977_003331"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--ammonia ligase"
                     /protein_id="HDI6043335.1"
                     /translation="MSAEHVLTMLNEHEVKFVDLRFTDTKGKEQHVTIPAHQVNAEFF
                     EEGKMFDGSSIGGWKGINESDMVLMPDASTAVIDPFFADSTLIIRCDILEPGTLQGYD
                     RDPRSIAKRAEDYLRSTGIADTVLFGPEPEFFLFDDIRFGSSISGSHVAIDDIEGAWN
                     SSTQYEGGNKGHRPAVKGGYFPVPPVDSAQDIRSEMCLVMEQMGLVVEAHHHEVATAG
                     QNEVATRFNTMTKKADEIQIYKYVVHNVAHRFGKTATFMPKPMFGDNGSGMHCHMSLS
                     KNGVNLFAGDKYAGLSEQALYYIGGVIKHAKAINALANPTTNSYKRLVPGYEAPVMLA
                     YSARNRSASIRIPVVSSPKARRIEVRFPDPAANPYLCFAALLMAGLDGIKNKIHPGEA
                     MDKNLYDLPPEEAKEIPQVAGSLEEALNELDLDREFLKAGGVFTDEAIDAYIALRREE
                     DDRVRMTPHPVEFELYYSV"
     gene            10192..11241
                     /gene="glnL"
                     /locus_tag="PN977_003332"
     CDS             10192..11241
                     /gene="glnL"
                     /locus_tag="PN977_003332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(II)"
                     /protein_id="HDI6043336.1"
                     /translation="MATGTQPDAGQILNSLINSILLIDDNLAIHYANPAAQQLLAQSS
                     RKLFGTPLPELLSYFSLNIELMQESLEAGQGFTDNEVTLVIDGRSHILSVTAQRMPDG
                     MILLEMAPMDNQRRLSQEQLQHAQQVAARDLVRGLAHEIKNPLGGLRGAAQLLSKALP
                     DPSLLEYTKVIIEQADRLRNLVDRLLGPQLPGTRITESIHKVAERVVTLVSMELPSNV
                     RLIRDYDPSLPELAHDPDQIEQVLLNIVRNALQALGPEGGEIILRTRTAFQLTLHGER
                     YRLAARIDVEDNGPGIPPHLQDTLFYPMVSGREGGTGLGLSIARNLIDQHSGKIEFTS
                     WPGHTEFSVYLPIRK"
     gene            11253..12662
                     /gene="glnG"
                     /locus_tag="PN977_003333"
     CDS             11253..12662
                     /gene="glnG"
                     /locus_tag="PN977_003333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(I)"
                     /protein_id="HDI6043337.1"
                     /translation="MQRGIVWVVDDDSSIRWVLERALAGAGLTCTTFENGAEVLEALA
                     SKTPDVLLSDIRMPGMDGLALLKQIKQRHPMLPVIIMTAHSDLDAAVSAYQQGAFDYL
                     PKPFDIDEAVALVERAISHYQEQQQPRNVQLNGPTTDIIGEAPAMQDVFRIIGRLSRS
                     SISVLINGESGTGKELVAHALHRHSPRAKAPFIALNMAAIPKDLIESELFGHEKGAFT
                     GANTIRQGRFEQADGGTLFLDEIGDMPLDVQTRLLRVLADGQFYRVGGYAPVKVDVRI
                     IAATHQNLEQRVQEGKFREDLFHRLNVIRVHLPPLRERREDIPRLARHFLQVAARELG
                     VEAKLLHPETEAALTRLAWPGNVRQLENTCRWLTVMAAGQEVLIQDLPGELFESTVAE
                     STSQMQPDSWATLLAQWADRALRSGHQNLLSEAQPELERTLLTTALRHTQGHKQEAAR
                     LLGWGRNTLTRKLKELGME"
     gene            12774..12884
                     /locus_tag="PN977_003334"
     CDS             12774..12884
                     /locus_tag="PN977_003334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YshB family small membrane protein"
                     /protein_id="HDI6043338.1"
                     /translation="MLESIINLVSSGAVDSHTPQTAVAAVLCAAMIGLFS"
     gene            complement(13074..14447)
                     /gene="hemN"
                     /locus_tag="PN977_003335"
     CDS             complement(13074..14447)
                     /gene="hemN"
                     /locus_tag="PN977_003335"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HDI6043339.1"
                     /translation="MSVQQIDWDLALIQKYNYSGPRYTSYPTALEFSEDFGEQAFLQA
                     VARYPERPLSLYVHIPFCHKLCYFCGCNKIVTRQQHKADQYLDALEQEIVHRAPLFAG
                     RKVSQLHWGGGTPTYLNKAQISRLMKLLRENFQFNADAEISIEVDPREIELDVLDHLR
                     AEDFNRLSMGVQDFNKEVQRLVNREQDEEFIFALLNHAREIGFTSTNIDLIYGLPKQT
                     PESFAFTLKRVAELNPDRLSVFNYAHLPTIFAAQRKIKDADLPSPQQKLDILQETIAF
                     LTQSGYQFIGMDHFARPDDELAVAQREGVLHRNFQGYTTQGDTDLLGMGVSAISMIGD
                     CYAQNQKELKHYYQQVDEQGNALWRGIALTRDDCIRRDVIKSLICNFRLDYAPIEQQW
                     DLHFADYFAEDLKLLAPLAKDGLVDVDEKGIQVTAKGRLLIRNICMCFDTYLRQKARM
                     QQFSRVI"
     gene            complement(14636..15145)
                     /gene="yihI"
                     /locus_tag="PN977_003336"
     CDS             complement(14636..15145)
                     /gene="yihI"
                     /locus_tag="PN977_003336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Der GTPase-activating protein YihI"
                     /protein_id="HDI6043340.1"
                     /translation="MKPSSSNSRSKGHAKARRKTREELDQEARDRKRQKKRRGHAPGS
                     RAAGGNTSSGSKGQNAPKDPRIGSKTPIPLGVTEKVTKQHKPKSEKPMLSPQAELELL
                     ETDERLDALLERLEAGETLSAEEQSWVDAKLDRIDELMQKLGLSYDDDEEEEEDEKQE
                     DMMRLLRGN"
     gene            15727..16359
                     /locus_tag="PN977_003337"
     CDS             15727..16359
                     /locus_tag="PN977_003337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HDI6043341.1"
                     /translation="MTNLNYQQTHFVMSAPDIRHLPSDTGIEVAFAGRSNAGKSSALN
                     TLTNQKSLARTSKTPGRTQLINLFEVADGKRLVDLPGYGYAEVPEEMKRKWQRALGEY
                     LEKRQSLQGLVVLMDIRHPLKDLDQQMIEWAVDSNIAVLVLLTKADKLASGARKAQLN
                     MVREAVLAFNGDVQVETFSSLKKQGVDKLRQKLDTWFSEMQPVEETQDGE"
     gene            complement(16741..>19486)
                     /gene="polA"
                     /locus_tag="PN977_003338"
     CDS             complement(16741..>19486)
                     /gene="polA"
                     /locus_tag="PN977_003338"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HDI6043342.1"
                     /translation="SSYLYRAYHAFPPLTNSAGEPTGAMYGVLNMLRSLIMQYKPTHA
                     AVVFDAKGKTFRDELFEHYKSHRPPMPDDLRAQIEPLHAMVKAMGLPLLAVSGVEADD
                     VIGTLAREAEKAGRPVLISTGDKDMAQLVTPNITLINTMTNTILGPEEVVNKYGVPPE
                     LIIDFLALMGDSSDNIPGVPGVGEKTAQALLQGLGGLDTLYAEPEKIAGLSFRGAKTM
                     AAKLEQNKEVAYLSYQLATIKTDVELELTCEQLEVQPPAAEELLGLFKKYEFKRWTAD
                     VEAGKWLQAKGAKPAARPQETSVADEAPEVTATVISYDNYVTILDEETLKTWIAKLEK
                     APVFAFDTETDSLDNISANLVGLSFAIEPGVAAYIPVAHDYLDAPDQISRERALELLK
                     PLLENEKALKVGQNLKYDRGILANYGIELRGIAFDTMLESYILNSVAGRHDMDSLAER
                     WLKHKTITFEEIAGKGKNQLTFNQIALEEAGRYAAEDADVTLQLHLKMWPDLQKHKGP
                     LNVFENIEMPLVPVLSRIERNGVKIDPKVLHNHSEELTLRLAELEKKAHEIAGEEFNL
                     SSTKQLQTILFEKQGIKPLKKTPGGAPSTSEEVLEELALDYPLPKVILEYRGLAKLKS
                     TYTDKLPLMINPKTGRVHTSYHQAVTATGRLSSTDPNLQNIPVRNEEGRRIRQAFIAP
                     EDYVIVSADYSQIELRIMAHLSRDKGLLTAFAEGKDIHRATAAEVFGLPLETVTSEQR
                     RSAKAINFGLIYGMSAFGLARQLNIPRKEAQKYMDLYFERYPGVLEYMERTRAQAKEQ
                     GYVETLDGRRLYLPDIKSSNGARRAAAERAAINAPMQGTAADIIKRAMIAVDAWLQAE
                     QPRVRMIMQVHDELVFEVHKDDVDAVAKQIHQLMENCTRLDVPLLVEVGSGENWDQAH
                     "
BASE COUNT         4677 a         4959 c         4776 g         5074 t
ORIGIN      
        1 ggtaatcagc aacttggcta tatcttcgcc gccacgctgt tttcactgtt cggcctgctg
       61 tttatgtgga tctgctactc gggcgtgaaa gagcgttatg tcgaaaccca acctaccaat
      121 ccggcgcaaa agcctggctt gttgcagtct ttccgcgcga ttgctggtaa ccgcccactg
      181 ttcattctgt gtattgccaa cctctgcact ttaggggcgt ttaacgtcaa gctcgccatt
      241 caggtctatt acacccagta cgtgctcaac gatcccatcc tgttgtcata tatgggattt
      301 ttcagcatgg gctgtatttt catcggcgtg ttcctgatgc ccggcgcagt cagacgtttt
      361 ggtaagaaga aggtctatat cggcggcctg ctgatttggg tgcttggcga tctgctcaac
      421 tatttctttg gcggcggttc ggtcagcttc gtggcgttct cctgcctggc attcttcggc
      481 tcagcgtttg ttaacagcct gaactgggcg ctggtttccg acaccgtcga gtacggcgag
      541 tggcgcaccg gtgtgcgttc ggaaggtacg gtctacaccg gtttcacctt ctttcgcaaa
      601 gtatctcagg cgctggcagg tttcttccca ggctggatgc tgacgcaaat cggctatgtg
      661 ccaaacgtcg cccaggctga ccacactatc gaagggttgc gccaactgat cttcatctac
      721 ccaagcgcac tggcagtagt caccatcgtg gcaatgggtt gcttctacag tctgaacgag
      781 aagatgtacg tccgcattgt tgaagagata gaagcccgta aacgcacggc gtaattataa
      841 taaacaacgc cctgcggggc gttataagga gtgattatgt ctgaccataa tccactgaca
      901 ttaagactga atctgcggga aaaaatcgcc tatggtatgg gcgacgtcgg ttcgaattta
      961 atgctctgca tcggtactct gtatctcctc aaattttata ccgatgaatt agggatgcct
     1021 gcttactatg gcgggattat ctttctggtg gcgaagtttt ttaccgcatt taccgatatg
     1081 ctcaccggat ttttactcga ctcgcggaaa aatattgggc ctaaaggcaa atttcgtccc
     1141 tttattttat atgcagcggt tccggcggcg ttaattgcaa cgctccagtt tatcgccacc
     1201 acctttagtt tgccggttaa aacgacgatt gccaccgcac tgtttatgat gtttggactt
     1261 tcatacagtc tgatgaactg ctcgtatggt gcgatgatcc cggccattac caaaaacccc
     1321 aatgagcgcg cgcagctcgc ggcttaccgc cagggtggtg caaccatagg gctattgatt
     1381 tgtaccgtgg cgtttattcc gttgcagtcg cttttttctg actcaaccgt cggttatgcc
     1441 tgtgcggcac tgatgttctc cattggcggc tttattttta tgatgctgtg ctacagaggc
     1501 gtcaaagagc attatgtgga caccacgcca accggacata aggccagtat tctcaaatca
     1561 ttttgcgcga tatttcataa tccacctttg ctggtattgt gcattgctaa cctgtgtact
     1621 ctggcggcat ttaatatcaa actggcgatt caggtctatt acacccagta tgtgctgaat
     1681 gatattaatt tattgtcgtg gatgggattt ttcagtatgg gatgcatcct cgtcggcgta
     1741 ttactggtgc cattaactgt aaaatgtttt ggtaaaaaac aggtttattt agctggcatg
     1801 gtgctatggg cggttggtga tatcctgaat tatttctggg gaagtaactc tttcactttc
     1861 gtcatgttct cttgtgtcgc cttttttggc acggcctttg taaacagcct gaactgggca
     1921 ctggtgccag ataccgtaga ttacggtgaa tggaaaaccg gtatacgtgc cgaaggttct
     1981 gtttataccg gttatacctt ctttcgtaaa atttcagccg cacttgctgg cttcttgcca
     2041 ggcattatgc tgacgcaaat cggttatgtt cccaacatcg cgcaaagcga tgcgacatta
     2101 cagggtttgc gtcagctcat ttttatctgg ccttgtgcgc tggcaatgat tgctgcattg
     2161 acgatggggt tcttttacac gctcaatgag aaacggtttg cactgattat tgaggaaatt
     2221 aaccaacgca aaaataaaga aattgagaca gaagaaaaaa cggcttccgt aacgttataa
     2281 atgtcaatag ccgctatttc catcctggtg ggtggcggcc tccctacgct taaaaatgga
     2341 cttattatga aaaagattaa tgcaataatt ctattatcct ctctgacctc tgcctcggta
     2401 tttgctggcg catacgtcga aaacagggag gcatataatc tcgcctccga tcagggcgag
     2461 gttatgctac gtgtgggtta taacttcgat atgggcgcgg gtattatgtt aaccaatacc
     2521 tacacctttc agcgagaaga tgaactcaaa catggatata acgaaatcga aggttggtat
     2581 ccgttattca aaccaaccga taaattaacc atccagcccg gtggcttaat taatgataag
     2641 agtatcggtt cgggtggtgc tgtttatctg gatgttaact acaaatttac tccatggttt
     2701 aacctgacgg tacgtaatcg ttttaaccat aataactaca gctcaaccga tttgaacggt
     2761 gatctggata ataatgacag ctacgagatt ggcaactact ggaactttat tattaccgat
     2821 aagttctcat atacctttga accgcattat ttttataaca ttaatgattt caatagcagc
     2881 aatggtacga agcatcactg ggaaatcact aacaccttcc gttatcgcat taatgagcac
     2941 tggttgccct atattgaatt gcgctggtta gatcgcaacg tagagccgta ccaccgcgag
     3001 caaaaccaga ttcgtatcgg gacgaagtat ttcttctgat gtaaaactgc cggatgcggc
     3061 gaaaacgcct tatccggcct acggttgata cggtttccgt tggccggatt aagccacatc
     3121 cggccattaa ttaagcttca gccgccagca tctctttctt acgttggcta atacgcttaa
     3181 ccagcattac ggaaaccacc gcaccacaga atgcgaagca ggccataatg ccaaacacga
     3241 ttttgtagcc aataatcccc ggatttaaat ccagaatgta gccatacaga ctgaagcaga
     3301 acatcgccgg ggcgtaacca ataaagctac ccaacgccat cgccgcgcct gttttatttt
     3361 cagcaatttt tgcttcgccg ataggtgcaa aaaatacggc tcgctgtgta aagactatcg
     3421 cgccaaagcc cagcgtacat gccatcccta aatagaccgg catactttcg tgcggcagca
     3481 taatcaacag tacgagcgca gcggtactga tgataaaggt gtagcataga tatttactcg
     3541 gcgatttcag gatcttatct gaaatcatgc caccaatcgg tccaccaatc attttcagac
     3601 agtattggtt aatgatgccg taagccccca ccagcgcaac aggcaataga tagatgtttt
     3661 tcaggaatgg aatgaagaat gtcaggccac agtaaaccgc gtaaacgaag aagacgttga
     3721 aagcgataag ccagatagtt ttgtctttca gcaccgaggt catactggtg tttttcgatg
     3781 ctccgtcttc ttttttcacc tcaacggacg gtgcctcttc tttgtcattc agaacaaaga
     3841 aaatgataat gcctacggca atcacaataa gggaatagaa ccagatgcct gctttaaaac
     3901 ccaataagcc actgccaaac caggtaaata ctgccaacgc agaaaatgcc accacggtat
     3961 cgacaatgcc acgccctgtt tcgaagaagc caaacaaccg accttgttgt tcgctattgc
     4021 ccaatcgact taccgacttg agcaagaccg gccagttcat catgtcgcaa gtaacgccaa
     4081 atagcgccca gacaaagagg atgccccaat accccggcat tgtcgtcaaa taaacaccca
     4141 gtaatcctgt cgcaatgagt gagaatgaca tggtgtatct gcgcggtagt ttatcggcaa
     4201 aataaataga cagaaaaaag cccactgtcg tgacaaatga gtttaccgac atagcattac
     4261 caatttgacc attggtcaaa tggaaatatt cctgcatcgg gatataaaac gcatctttca
     4321 gcgacggtaa tttataaatt gtaccgccac acagtgttaa tagactaaat aacgcccatt
     4381 tctttttcgt gagcataaca tcccctaagc gatttttaaa atcactgttt taaaggcaat
     4441 aagataccga ccattcatat ggaatggttt tattaacagc gtatgagtta aaacttatta
     4501 gtggttcaga agatggtttg cctcttgttt gatttgttgt agtacgttac gcacatgatt
     4561 tatctgatta attgcatctt ctttttcttt acgtaaccgc gcatccagta aatgattttc
     4621 tggcaatggt gtttcactca tctggcgata agggatccac ggattatcat cttcgtcttc
     4681 aaagcggaac gccggcgcat agtaatcaac ctcttcttcc aggccatatg ccgtcacctg
     4741 cggctcatca tcacccaggc agataaggtg cgttaataac gctttgaacg gcatatcacc
     4801 ctgacctgaa atacaggctt tatgacccag gccaccctct tctttaacga tcaaggcatc
     4861 tttgatatgg acctgggtaa tatgcggtgc catcgttttt aaagcgtcga tgggatgctc
     4921 atttgcattg atcatgttcg caaaatcaaa cagtaaggaa agagattcca tctcactttc
     4981 tttgaccagc gacaccagct catggctttt taaatcttca tgctgttcga tcgtaaaagt
     5041 caggccgctg tcctgatacg tttctcgtac ataggcaatg tcgttagcga taatcgataa
     5101 tacatcgcgc aaattacctt cataacgcgg ataaaaacgt acggaggatg ccccagtttt
     5161 caacgcgatg gcgacggctt cgtcaataga ttttttatct gaggcgctgg tttcaatatg
     5221 aatatcaagg ttcagacggc gggctttatc cccaaaggca gagagttctt tatcgtccat
     5281 attaccaaga gaaaaacgct ctccatccag aacatggatt ttgacgccac gtagattatt
     5341 ttcacttgcg atatcaagta aatcagcagg caacactctt tccagtcgca tatttaaatg
     5401 aaaggcataa gcatgtaaat aaagaggaag agtgtcgaca cgttgtagaa tctttcttgc
     5461 gttatttatt gtcaccataa aacacctaca gattaatgat gaggtctacg gcctcaaatc
     5521 gccaatattc aatatctatc tggacaatat tgccctgctg gtcggcgcga tgcttttcaa
     5581 ttaagatcgc tggcataccc gcagaaccgc caagatactt gctgatatcg cccggcattc
     5641 tgacgggttt gaaaatcaat ttttttacta ccgtctcttt ttggtagcac ttttcccaga
     5701 ctgcagaaaa tgagtggttt tcaagttgtt caataaaacc cggggcaact tcaggattaa
     5761 tatatgtttc gtgatagaaa actttatgtc cttccagcgc cccccagccg gtgatgcgat
     5821 agagttcagt tgatggtgtc actgcaatca gtggcgcgag cgtctcggga atatcgctgg
     5881 tacggttttt ctcggtaaac ccccaggaag gctctcgtcc ttgctcaatt gcggcccgct
     5941 gaaagctcgc tgacagctcc ggactgtaat taaatcgcgg ctgggtcacg aaccacccct
     6001 tacgatcctt acggaagatt ttggattctg cctcgaggtt caacaacgcc tggcgcagcg
     6061 tcatacgttt aatgcccagc agttctccca gctctctttc tgaagggagt tttccacccg
     6121 gcgtggtgat gccatctttc aaccaccgat ccagtttctc ttttgcattt tctacggtgg
     6181 attgattctc cgccattgtt taatcctttt ggtttaaacc agatgaagca tcttacgctt
     6241 acgccaaaaa agaaagcaat taacggtgta tgactttgca gataattgtg acaaagctct
     6301 ctattgacgt aatcacagac ttataaggga gatactcgca ttggtgtgcg ctcacgacag
     6361 gtaaaaaaaa acctgccagc gatggcaggt tttttataac taaaaaacga aattaatcgt
     6421 ctttcggtgc gcggttggcg cgacgacgat cgttttccgt caggtgacgt ttacgaatac
     6481 ggatagaggt cggagtcact tctaccagtt cgtcatcatc gatgaactcc agagcttgtt
     6541 ccagagtcat gcggataggc ggaaccagaa caacggcttc gtcagtaccg gaagcacgca
     6601 tgttggtcag tttcttaccg gtcaggcagt ttacagtcag gtcgttagag cggctatgaa
     6661 taccgataat ctgaccttcg taaacttctg caccgtgacc gaggaacagc ttaccgcgat
     6721 cttgcagacc gaacagcgcg aacgcgaccg ctttaccctg accgttagag atcagtacgc
     6781 cgttctgacg ctgacccact tcacccggac gtacgtcgtc gtagtggctg aaggtggagt
     6841 acagcagacc agtaccggaa gtcatggtca tgaactcaga acggaagcca atcagaccac
     6901 ggcttgggat cacgtagtcg agacgtacgc ggcctttacc gtctggattc atgtttttca
     6961 ggtcgccttt acgttcgccc agcgcctgca ttacagaacc ctgatgctgt tcttcaacgt
     7021 ccagagtcac gttttcatac ggctcttgtt tacgaccgtc gatttcacgg aagataactt
     7081 tcggacggga taccgccagt tcgaaacctt cacgacgcat gttttcgatc agaacagaca
     7141 ggtgcagttc gccacgacca gaaacgcgga acgcatcggc gtcttcggtt tcttctacgc
     7201 gcagcgcaac gttgtgtacc agttctttgt tcagacgatc caggatctga cgagacgtta
     7261 cgaacttacc ttctttaccg cagaacggcg aggtgttaac gcagaagaac atagaaacgg
     7321 tcggctcatc aacggagagt gccggcagcg cttcaacgtt ttgcgtgtcg caaacggtgt
     7381 cagaaatgtt cagttcgcca aggcccgtga tcgcaacgat atcgccagct tccgccagat
     7441 cggtttcgat acgttccaga ccgaggtggc ccagcacttt accgactttc gcgttgcggg
     7501 ttttgccttc gctatcgatg atagtgacct gctggttcgg cttcacttta ccgcgcttga
     7561 tgcggccaat gccgataacg ccaacatagc tgttgtaatc gagctgagaa atctgcatct
     7621 ggaacggacc gtcaaggtca acgtccggcg caggaacgtg gtcaacaatc gcctggtaca
     7681 gcggggtcat gtcttccgcc atatcttcgt ggtccagacc cgcgataccg ttcagcgcag
     7741 aagcgtaaac gatcgggaag tccagctgct cgtcggtcgc gtcgaggtta acgaacagat
     7801 cgaatacctg atccacaacc caatcaggac gcgcgccagg gcggtcaact ttgttgataa
     7861 caacaatcgg cttcaggccg taagcaaacg cttttttggt tacgaagcgc gtttgcggca
     7921 tcgggccgtc aaatgcgtca accaccagca gcactgaatc taccatggac attacacgtt
     7981 caacttcacc accgaagtcg gcgtgccccg gggtatcaac gatgttgata cggtaatcat
     8041 tccatttgat agcggtgttt ttcgcgagga tggtaatccc acgctctttc tccaaatcgt
     8101 tggagtccat cacgcgctct tgggtttcgg cacgagagtc gaacgtaccg gattgttgga
     8161 gcagcttgtc taccagggtg gttttaccat ggtctacgtg cgcgatgatg gcgatattac
     8221 gcaatttttc gatcacaact ttgcctcagg cattagaaat agcgcgttat tgtacacgga
     8281 ttaatcgcac tacaaaacag gatcacaaac atcctccgca aacaagtatt gcagagtccc
     8341 tttgtgatcg ctttcacgga gcataaaaag ggttatccaa aggtcattgc accaatatgg
     8401 tgcttaatgt ttccattgaa gcactatatt ggtgcaacat tcacatcatg gtgcagccct
     8461 tttgcacgat gttaagcatg ataacgcctt ttaggggcaa tttaaaagtt ggcacagttt
     8521 tcgctttatc ttttttacgg cgacacggcc aaaataattg cagatttcgt taccacgacg
     8581 accatgacca atccaggaga gttaaagtat gtccgctgaa cacgtactga cgatgctgaa
     8641 cgagcacgaa gtgaagtttg ttgatttgcg cttcaccgat accaaaggta aagaacagca
     8701 cgtcactatc cctgctcatc aggtgaatgc cgaattcttc gaagaaggca aaatgtttga
     8761 cggctcctcg attggcggct ggaaaggcat taatgaatcc gatatggtgc tgatgccaga
     8821 cgcatccacc gcagtgattg acccgttctt cgccgattct accctgatta tccgttgcga
     8881 catccttgaa cctggcaccc tgcaaggcta tgaccgtgac ccgcgctcta ttgcgaagcg
     8941 cgccgaagat tacctgcgtt ctactggcat tgccgacacc gtactgttcg ggccagaacc
     9001 tgaattcttc ctgttcgatg acatccgttt cggatcgtct atctccggtt cccacgttgc
     9061 tatcgacgat atcgaaggtg catggaactc ctccacccaa tacgaaggtg gtaacaaagg
     9121 tcaccgtccg gcagtgaaag gcggttactt cccggttccg ccggtagact ctgctcagga
     9181 tattcgttct gaaatgtgtc tggtgatgga acagatgggt ctggtggttg aagcccatca
     9241 ccacgaagta gcgactgctg gtcagaacga agtggctacc cgcttcaata ccatgaccaa
     9301 aaaagctgac gaaattcaga tctacaaata tgttgtgcac aacgtagcgc accgcttcgg
     9361 taaaactgcg acctttatgc caaaaccgat gttcggtgat aacggttccg gtatgcactg
     9421 ccacatgtcg ctgtctaaaa acggcgtcaa cctgttcgca ggcgacaaat acgcaggtct
     9481 gtctgagcag gcgctgtact atattggcgg cgtaatcaaa cacgcgaaag cgattaatgc
     9541 cctggcaaac ccgaccacca actcttacaa gcgtctggtc ccgggctacg aagcaccggt
     9601 aatgctggct tactctgcgc gtaaccgttc tgcgtctatc cgtattccgg tggtttcttc
     9661 tccgaaagca cgtcgtatcg aagtacgttt cccggacccg gcggctaacc cgtacctgtg
     9721 ttttgctgcc ctgctgatgg ccggtcttga cggtatcaag aacaagatcc atccgggcga
     9781 agccatggac aaaaacctgt atgacctgcc gccggaagaa gcgaaagaga tcccacaggt
     9841 tgctggttct ctggaagaag cactgaacga actggatctg gaccgcgagt tcctgaaagc
     9901 cggtggcgtg ttcactgatg aagcgatcga tgcgtacatc gctctgcgtc gcgaagaaga
     9961 tgaccgtgtg cgtatgactc cgcatccggt agagtttgag ctgtactaca gcgtctaata
    10021 gttgaagttg tactacccgg cgcaacaacg ctgggattta gttgccgtgg aaactttcag
    10081 cccatctctg catgggcttt tttctccgtc aattctctga tgcttcgcgc tttttatccg
    10141 taaaaagcta taatgcacta aaatggtgca acctgttcag gagactgctt tatggcaaca
    10201 ggcacgcagc ccgatgctgg gcagatcctc aactcgctga ttaacagtat tttgttaatt
    10261 gatgacaatc tggcgatcca ttacgccaac cccgccgcgc aacaactgct cgcccaaagc
    10321 tcccgcaaat tgtttggtac gccgttaccg gaactgttga gctacttctc attaaatatc
    10381 gagctgatgc aagaaagtct ggaggcgggg caaggcttta ccgataacga agtgacgctg
    10441 gtcatcgacg ggcgctcgca tatcctttct gtgacggctc agcgtatgcc ggacggcatg
    10501 atcctgctgg agatggcacc gatggataac cagcgccgct taagtcagga acagctacag
    10561 cacgcccagc aggttgctgc ccgtgattta gtgcgcggcc tggcacatga gattaaaaat
    10621 ccgcttggcg gtttacgtgg cgcggcgcag ctgctcagca aagcattacc tgacccgtca
    10681 ctactcgaat ataccaaagt gattatcgaa caggcggacc ggctgcgaaa tctggtcgac
    10741 cgtctgttgg ggccgcagct gcccggtacg cgtattaccg aaagtattca caaagtggct
    10801 gaacgcgtgg tgacgctggt gtcgatggaa ctgccgagca acgtgcggtt gattcgggat
    10861 tacgacccca gcctgccgga actggcgcac gacccggatc aaattgaaca ggtcttgctg
    10921 aatattgtgc gcaatgcgct acaggcgctg gggccggagg gtggtgaaat cattctgcgt
    10981 acccgcaccg cgtttcaact gaccttacac ggcgagcgtt atcggctggc ggcgcgaatt
    11041 gatgtggaag ataacgggcc aggtattccg cctcatttgc aggatacgct gttttacccg
    11101 atggtcagcg gccgcgaagg tggcaccggg cttggcttat ccatcgcccg taatttgatt
    11161 gatcagcatt caggcaaaat tgaatttacc agttggccag gtcataccga gttctcggtt
    11221 tacctgccta tcaggaaata aaggtgacgt ttatgcaacg agggatagtc tgggtagtcg
    11281 atgacgatag ttccatccgt tgggtgcttg aacgtgcgct cgctggagcg ggtttaacct
    11341 gtacgacatt tgagaacggc gcggaagtac tggaggcgct ggcgagcaaa acgccggatg
    11401 tgctgctttc agatatccgt atgccgggaa tggacgggct ggcgctgctc aagcagatta
    11461 aacagcgcca tccgatgctt ccggtcatca ttatgaccgc acattccgat ctggatgctg
    11521 ccgtcagcgc ctatcaacaa ggggcgtttg attatctgcc caaaccgttt gatatcgacg
    11581 aagccgtcgc gctggttgag cgcgccatca gtcattacca ggaacagcag cagccgcgta
    11641 atgttcagct taacggccca acgaccgata tcatcggcga agcgccagcc atgcaggacg
    11701 tgttccgtat tatcggtcgg ctttcgcgtt cttctattag cgtgctgatt aacggcgaat
    11761 ccggcaccgg taaagaactg gtcgctcatg ccctgcatcg ccacagtccg cgagccaaag
    11821 cgccatttat cgcgctgaat atggctgcta tcccgaagga tttgatcgaa tcagaactgt
    11881 ttggtcacga gaaaggcgca tttaccggcg cgaataccat tcgtcagggg cgttttgaac
    11941 aggctgatgg cggtacatta ttcctcgatg aaattggcga tatgccgctg gatgtgcaga
    12001 cgcgtttgct gcgcgtgctg gcagacggtc agttttatcg cgttggcggc tatgcgccgg
    12061 tgaaagtgga tgtgcggatt atcgctgcca ctcaccagaa tcttgaacag cgggtgcagg
    12121 aaggtaagtt ccgtgaggat ctgttccacc gcctgaacgt tatccgcgtt catctgccgc
    12181 cattgcgtga gcgtcgggaa gatattcccc gtctggcacg ccatttttta caggttgccg
    12241 cgcgcgaact gggcgtagaa gcgaagttgc tgcatccgga aaccgaagcc gcgctgacgc
    12301 gcctggcgtg gccaggcaac gtgcgccagc tggaaaacac ctgtcgctgg ctaacggtga
    12361 tggccgccgg gcaggaagtg ttgattcagg atttgcccgg tgaactgttt gaatcaacgg
    12421 ttgcggagag tacttcgcaa atgcaaccgg acagttgggc gacactttta gcgcagtggg
    12481 cagacagagc gctgcgttcc ggtcatcaaa acctgctttc cgaagcgcag cctgagctgg
    12541 agcggacgtt actgacgacc gcgttgcgac atacgcaggg gcataaacag gaagcggcgc
    12601 ggctactcgg ctggggccgc aacaccctga cgcgtaagtt aaaagagcta gggatggagt
    12661 gattcacggc ttgtgagtaa agattgatta ttgagcgcaa attgctggta ttttgcgctt
    12721 tactgttccg ataagttcag tatgatcttg cccggaaaac ggggagagtc attatgctgg
    12781 aatcaataat taatctggta tcgagtggcg cagttgacag ccacacacca caaactgccg
    12841 ttgctgccgt gctgtgtgcc gcgatgattg ggctgtttag ttgagtttca gattgctgat
    12901 gaagtgctct ttgttcatgc cggatgcggc gtgaacgcct tatccggcct gcaaaagcaa
    12961 tattttcaac aggttttgta ggcctgataa gcgtagcgca tcaggcaatc ttgttctcag
    13021 cacattgatg gcgctttcgt tttctacttt gtaaacgaag cgccattcac tatttaaatc
    13081 acccgtgaga actgctgcat ccgcgctttc tggcgcagat aggtatcaaa gcacatgcaa
    13141 atgttgcgga tcagcaagcg acctttcgcc gtcacctgaa tccccttctc atccacatcc
    13201 accagcccat cttttgctaa cggggcgagc agcttgagat cttccgcaaa gtaatcagcg
    13261 aagtgcaaat cccactgttg ctcaatggga gcgtaatcca gacggaagtt gcagatgagc
    13321 gacttaatca catcgcggcg aatacagtca tcacgcgtta gcgcaatacc acgccacagc
    13381 gcgttgcctt gttcatccac ttgctgatag tagtgcttca actctttctg gttctgcgcg
    13441 tagcagtcgc caatcatact gatggcggaa acgcccatcc ccagcagatc ggtatcgccc
    13501 tgagtggtgt agccctggaa gttacgatgc agcacgcctt cacgctgggc caccgccagc
    13561 tcgtcatccg ggcgggcaaa gtgatccatc ccgataaact gatagcccga ttgcgtcagg
    13621 aaggcaatgg tttcctgcag gatatcgagt ttttgctgcg gactcggcag gtcagcatct
    13681 ttgattttgc gctgagcagc aaaaatggtc ggcaaatgcg cgtagttaaa gacgctcaga
    13741 cgatcggggt tcagctccgc cacacgtttc agggtaaagg caaaactttc cggcgtctgt
    13801 ttcggcagac cgtaaatcag gtcgatgttg gtggaggtaa agccaatctc acgcgcatgg
    13861 ttaagcagtg caaagatgaa ctcttcatcc tgctcgcggt taaccagacg ctgtacttct
    13921 ttgttgaagt cctgcacgcc catgctcagg cgattaaagt cctcggcgcg taaatgatcg
    13981 agtacatcca gttcgatttc gcgcggatcg acttcgatcg aaatctccgc atcggcattg
    14041 aactggaagt tttcgcgcag cagcttcatc agacggctga tttgcgcttt gttcagatac
    14101 gtcggcgtac caccgcccca gtgcagctgg ctcaccttgc gcccggcaaa aagcggtgca
    14161 cgatggacga tttcttgctc cagcgcgtcc agatactgat cggccttgtg ctgctggcga
    14221 gtaactatct tattgcaacc gcagaagtaa caaagcttat ggcagaacgg aatatgtacg
    14281 tagagagata atggacgctc aggatagcgc gccacggctt gtaaaaacgc ctgttcgccg
    14341 aagtcttctg aaaactccag cgcggtcggg tacgaggtgt atcgtggccc ggaatagtta
    14401 tatttctgga tcagggccag atcccagtcg atttgctgta cagacatgct cactccttcc
    14461 gatggcgtct ctggcggcta cggcgtaccg gcggatgccc gtttcgggtc atcagccggt
    14521 tgcgcagcca cttttgtcgc cgcgacaacc gctgtagttt aacgaataac cacaccagat
    14581 aacatataac cggaagggtt atcagcagaa cgggaaggcc cacggcggca atccgttagt
    14641 tgccccgcag cagacgcatc atgtcttctt gcttctcgtc ttcttcctct tcttcgtcat
    14701 cgtcataaga gaggccgagt ttctgcatca actcatcaat acgatccagt ttagcatcca
    14761 cccaggattg ctcttcggcg ctcagggttt cgcctgcttc cagacgttcc agcagcgcat
    14821 ccagacgctc atccgtttcc agtaattcca actccgcctg cggtgaaagc ataggtttct
    14881 cactcttcgg tttgtgctgt ttggtgactt tttcagtcac gcccaatgga ataggggttt
    14941 tactgccaat acgtggatct tttggtgcgt tctggccttt gctgcctgac gaggtgttac
    15001 cgcccgctgc gcggctgccc ggcgcatgac cacgacgttt tttctgacgc ttacggtcac
    15061 gagcttcctg atccagctcc tcgcgtgttt ttcgacgcgc ttttgcatga cctttgctgc
    15121 gtgagtttga agatgatggt ttcataatgg ttcgtctttt agccgtttta ttcagtatag
    15181 atttgcggcg gaatctaaca gaaagcaagc aaagaaaaaa ggcgacagat tactctgtcg
    15241 ccttttttcc tgactcataa cccttaacgg gtcttacaat ccttgcaggc cgccaacaca
    15301 ccctgttttt ccattagccg gaacaatccg tgttgattcc atttccgttt aattacgtct
    15361 ccggacgttt gtcttcctga caatcctctg tcttcgcctc ctggcgctcc tgaccctcat
    15421 cctgagtcat tgttcctgtt agcgtcctcg ctctatgggt gctactttac gcctttgctt
    15481 taaacaaaca atcaaccgcc atcagtattt gcacattttc agagttttat ataaaagcaa
    15541 ttaactgatt tatatagctt atttgacatg cgtcgcagaa ttttctctgc tatttcgcat
    15601 aaagactatc ggcaatgtct cacaaagcgt atggctttta cttacaacgc tcacgccgaa
    15661 aaatccgcct tttattatgg ttcaggtata atcgccgcaa agctttgact gacggagacg
    15721 accgctttga ctaatttgaa ttatcaacag acgcattttg tgatgagtgc gcctgatatt
    15781 cgccacctac cttccgatac cggaattgaa gtggcttttg caggccgttc caacgcaggt
    15841 aaatccagcg cgctgaacac gctgactaac cagaaaagcc tggcgcggac ctcaaaaacc
    15901 ccagggcgca cccagcttat caacctgttt gaagtggctg acggcaagcg tctggttgac
    15961 ttgcctgggt acggttatgc ggaagtcccg gaagagatga agcgcaaatg gcagcgcgcg
    16021 ctcggcgagt acctcgaaaa acgtcagagc ctgcaaggtc tggtggtgct aatggatatt
    16081 cgccatccgc tgaaagattt ggatcagcag atgattgagt gggcggtaga cagcaatatc
    16141 gccgtgctgg tgctgctgac caaagcggac aaactggcaa gcggcgcacg taaagcgcaa
    16201 ttgaatatgg tgcgtgaagc ggtgctggcg tttaacggtg atgtgcaggt tgaaaccttc
    16261 tcttcactga agaaacaagg cgtggacaag ctgcggcaga aactggatac ctggtttagc
    16321 gagatgcagc ctgtagaaga aacgcaggac ggcgaataat tttcttgcct taatgcttgt
    16381 gccggatgtg gcgtatccgg cccgtaaatt caatagatta cacttctacc gttggcctga
    16441 taaacatggc gcatcaggca ttacggatct tttctttcgc ccaataaaaa acgccccagt
    16501 cattactgac tggggcggct aaaatattca gccaaatccg attacgtgaa gtaaaaggtc
    16561 tgaaagatag aacatcttac ctctgtaccc tacgcgacta actttactct tttttgttca
    16621 gttcagaaag cactttttgt aatttttttt cagttgttgc ataggaattt ctaatagcca
    16681 tcacaaaacg tgacagctta tgttgcttac ttacgaaaaa agggcatgtt caggcgaatc
    16741 ttagtgcgcc tgatcccagt tttcgccact ccccacttcc accagcaacg gcacatccag
    16801 acgggtacag ttttccatca gttgatgaat ctgcttcgcg acggcatcga catcatcttt
    16861 atgaacttca aataccagtt catcgtgtac ctgcatgatc atacgtacac gcggttgctc
    16921 agcctgtagc cacgcatcaa cggcaatcat cgcccgtttg ataatgtcgg cggcggttcc
    16981 ctgcattggc gcgttaatgg ctgcacgttc agccgctgca cgacgagcac cattgctgga
    17041 tttgatatcc ggcagataca gacggcgtcc gtccagcgtt tcaacgtagc cctgctcttt
    17101 cgcctgagca cgggtgcgtt ccatatactc cagcacgcca gggtagcgtt cgaagtaaag
    17161 gtccatgtac ttctgcgctt ctttacgtgg aatgttcaat tgccgcgcca ggccgaaagc
    17221 actcatgcca taaatcagac caaagttgat cgctttcgcg ctacggcgtt gctcgctggt
    17281 gacggtttcc agtggcaaac caaacacttc tgccgccgtt gcacggtgga tatcttttcc
    17341 ttccgcgaat gcggtcagca agcctttgtc acgcgagaga tgcgccataa tgcgcagttc
    17401 aatctgcgag tagtccgccg agacaatcac ataatcctct ggcgcaataa acgcctggcg
    17461 gatacgacga ccttcttcat tacgcaccgg aatgttttgc aggttaggat cggttgacga
    17521 taaacgtccc gttgcggtta ctgcctggtg ataagaggta tgtacacgcc cggttttcgg
    17581 gttgatcatc aacggcagct tgtcggtgta ggtcgatttc agcttcgcca gaccacgata
    17641 ctccagaatc acttttggca acggatagtc cagcgccagt tcttccagta cctcttccga
    17701 cgttgacggc gcgccacccg gcgttttctt cagcggttta atgccctgtt tttcaaacag
    17761 aatggtttgt aactgcttgg tggaagaaag gttaaattct tcacctgcaa tttcatgcgc
    17821 tttcttttcc agctcagcca gacgcaaggt gagctcttca gagtgattgt gcagcacttt
    17881 cggatcgatc ttcacaccgt tacgttcaat gcgtgaaagc accggcacca gcggcatttc
    17941 gatattctcg aagacgttca acggcccttt gtgtttttgc agatccggcc acattttcag
    18001 atgcaactgc aaggtgacat ctgcatcttc ggcggcgtaa cgtccggctt cttcgagggc
    18061 aatctggtta aaggtcagtt gatttttgcc tttacccgca atctcttcaa aagtgatggt
    18121 tttgtgcttc aaccaacgtt ccgcgaggct gtccatatcg tgacgcccgg caacgctatt
    18181 gagaatgtag gactccagca tggtatcaaa cgcaatccca cgcagctcaa ttccgtaatt
    18241 cgccagaata ccgcggtcgt atttcaggtt ttgcccgacc ttcagcgcct tttcattttc
    18301 cagcagcggt tttagcaact cgagtgcacg ctcgcgagag atttgatcgg gcgcatcaag
    18361 ataatcatga gcaaccggaa tatatgccgc tacgcctggc tcgatagcaa aagaaagccc
    18421 gaccaggtta gcagagatgt tatcaaggct gtcggtttcg gtatcaaatg caaataccgg
    18481 cgctttttcc agcttcgcaa tccacgtttt cagtgtttct tcatcaagga tggtgacgta
    18541 gttgtcataa gaaatcaccg ttgccgtcac ttctggcgct tcgtctgcaa cactggtttc
    18601 ctgcggcctg gctgcgggtt tcgccccttt agcctgtaac catttgcccg cttcgacatc
    18661 agcagtccag cgtttgaact catacttttt gaacagcccc aacaactctt ccgccgccgg
    18721 tggctgcact tccagttgtt cacaggtcag ctccagttca acgtcggttt taatcgtcgc
    18781 cagctggtat gagagataag caacttcttt gttttgctcg agcttcgctg ccattgtttt
    18841 cgcgccacgg aagctcaacc cagcaatttt ttctggctcg gcatacagcg tatccagtcc
    18901 gccaagacct tgcagcaatg cctgcgcggt tttttcaccg acgcccggta cgccaggaat
    18961 gttatcagag gagtcaccca tcagcgccag gaaatcgatg atcagttctg gcggcacgcc
    19021 gtacttattc accacctctt ccgggccgag gatggtattc gtcatggtat tgataagcgt
    19081 aatatttggc gtcaccagct gcgccatatc tttatcgcca gtgctgataa gcaccggacg
    19141 cccggctttt tcggcttcgc gcgccagagt accgataacg tcgtccgctt ctacgccaga
    19201 aaccgccagc agcggcagtc ccatcgcttt aaccatcgcg tgcagaggtt cgatttgtgc
    19261 tcgcagatcg tccggcattg gcgggcgatg tgatttgtaa tgttcaaaca gttcatcacg
    19321 aaaggttttt cccttggcgt caaagaccac cgctgcatgc gtcggtttat attgcatgat
    19381 cagactgcgc agcatgttga ggacaccata catcgcacca gtcggctcgc ctgcgctgtt
    19441 agtcagcggg ggaaacgcgt gatatgcgcg ataaagataa gatgaa
//
LOCUS       DAMZFT010000090        19355 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.090, whole genome shotgun sequence.
ACCESSION   DAMZFT010000090 DAMZFT010000000
VERSION     DAMZFT010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19355
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.090"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(<1..1851)
                     /locus_tag="PN977_003339"
     CDS             complement(<1..1851)
                     /locus_tag="PN977_003339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001705208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HDI6043343.1"
                     /translation="MSGQFSVGVVIGGMIGSTFRSAMSGTRRALDSLSDTSRRLQERQ
                     NALTRATERYGQLGSSRMQHLNSELLRVSRTMEQIERQQRRLSAASATSDALKANRMA
                     LYGQGIEAYGMAQTVYHTVSPAVQQSMSFQDKMIDMSITAKYDNKTRDALAGQIKGWA
                     QKYNQYQDELQEAVGSLISDNIDNLSDIGFLMPDIARAATATRTSSQDWAKVAAVWQN
                     SLKGAARDFSAVQNIMAYAGDQGSFEIPDQVKWMQSLAPMMAGIASGKEAVAEIGASL
                     QIAKIGAGSTDEAANNFKNFLTKIFARDTQKQFADLGIDLQGSVASYKAAGISPIEGM
                     LSVIERYLNAKSPEALAGFKSAMKIKNDTARDEALQALAKNFGLGDMFADMQVMAFIR
                     PMLANMDRYREIRAGALRAADNDLLASAYDQRLKSPLEATKALMVSSRDLAITLGDQL
                     APSFISLTQELIPLIQGAKHWVATHPQFVSGAFKLISALLAIKIATVGLKLGLNLLIS
                     PFVNVWKTAVLLRTNWHRLTTALGEGGKLRWLVTGFSRLTSGGLKLSKVLAGSLVRGF
                     MSAARAVLWIGRALMMNPIGLVITAVAAAAYLIYRNWGTVSGWFKQRWADI"
     gene            complement(1927..2085)
                     /locus_tag="PN977_003340"
     CDS             complement(1927..2085)
                     /locus_tag="PN977_003340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001202894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GpE family phage tail protein"
                     /protein_id="HDI6043344.1"
                     /translation="MRATKALTQAQGLLARWFRFQPGEIDALDTDDLEMWLEQAEEQI
                     KSEYGDKS"
     gene            complement(2009..2344)
                     /locus_tag="PN977_003341"
     CDS             complement(2009..2344)
                     /locus_tag="PN977_003341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000084214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein"
                     /protein_id="HDI6043345.1"
                     /translation="MSQTPSDTFKLSYPFTTAAGTRIELVELKRLTVKDLKQVRKISK
                     NPADWDEPLIARSTGLLPEDLDNMDLADYLQLQKRFQLITGMGESDKGADAGAGAAGE
                     MVPVSAGGD"
     gene            complement(2442..2723)
                     /locus_tag="PN977_003342"
     CDS             complement(2442..2723)
                     /locus_tag="PN977_003342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000110114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6043346.1"
                     /translation="MSVELTDKGGRCAALGMSNGTWFTLLDIPGVETLFNTRKTNDPI
                     DCTRSKARRLADLIEAWEPPDHWFSGTGKSEGKTLLIAFLRNCKGFRTC"
     gene            complement(2726..3250)
                     /locus_tag="PN977_003343"
     CDS             complement(2726..3250)
                     /locus_tag="PN977_003343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major tail tube protein"
                     /protein_id="HDI6043347.1"
                     /translation="MMSKIEINRITNANIYLDGTNLLGRAEEVKLPDVSMIMQEHKAL
                     GMVGKVELPAGFDKLEGEIKWNSFYRDAMLSAANPYRSLALQCRSSVQRYSSQGLIDE
                     IPLVTFLTIMFKKNPLGTFKQHENAEFSSSFTCTYIRQVLDGEELLQLDYLANIFRVG
                     GVDQLTDYRINIGG"
     gene            complement(3247..4674)
                     /locus_tag="PN977_003344"
     CDS             complement(3247..4674)
                     /locus_tag="PN977_003344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001702162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath subtilisin-like
                     domain-containing protein"
                     /protein_id="HDI6043348.1"
                     /translation="MAANYLHGPETIEVENGARPVKTVKSAVIGLIGTAPMGDVNTLV
                     QCLSEKDAAAFGSQFTGFTIPQALDAIYDHGAGTVLVINVLDPAKHKTAIQGEVVTFD
                     KSTGQARLAHPVVANVVVKNSEGSTTHTANTDYRVDAQAGVLTNLGKAIEAGGSVKVS
                     YEYADPSKVTAADIIGGVNSAGNRTGMKLLNDSFNLYGYFAKILIAPVFCTQKSVAVE
                     LIAMTEKLGAVTYIDAPVGTTFAQALAGRGPEGTINFNTSSDRVRLCYPHVKVYDPVT
                     NTERLEPLSQRAAGLRARVDLDKGYWWSSSNQEILGITGVERQLSAMIDDPQSEVNLL
                     NEQGITTVFSSYGSGLRLWGNRTAAWPTVTHMRNFENVRRTGDVINESIRYFSQQYID
                     MPITQALIDALTESVNAYGRKMTGDGAVLGFRCWFDPARNPETELAAGHLLLSYKYTP
                     PPPLERLTFETEITSEYLLTLKGGN"
     gene            complement(4664..4915)
                     /locus_tag="PN977_003345"
     CDS             complement(4664..4915)
                     /locus_tag="PN977_003345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001513985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6043349.1"
                     /translation="MKAYRYSGPASGVTLSDGTEILLWPGKTFSLPEEHDYVKVLVAL
                     KHLTPVPEETKLAGTPAVQSPKRRSSSDSEVKTEDTHGS"
     gene            complement(4915..5379)
                     /locus_tag="PN977_003346"
     CDS             complement(4915..5379)
                     /locus_tag="PN977_003346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016191683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gp37 family protein"
                     /protein_id="HDI6043350.1"
                     /translation="MNVLPVLDAVLARLRENLPQLQVEYFPEKPAEYRLNHPVGALLL
                     SYAGSRFDRPDDTGAVIQPQTIQLCVTVVFRQLNGKKGAINVLDAVRRILGGYTPPGC
                     RRRIWLTREVFIGEVRGLWQYALDFATESVFIEDSDLPSGPLLTEVNYEESE"
     gene            complement(5379..5825)
                     /locus_tag="PN977_003347"
     CDS             complement(5379..5825)
                     /locus_tag="PN977_003347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000271671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1320 domain-containing protein"
                     /protein_id="HDI6043351.1"
                     /translation="MYCTLEDLLAQVPERTLIELTSEDMDFDSPATVNTRVVDSCIRY
                     ADELIDAHLRGRYILPLAEIPTVLRDIAITLVRYRLYARRPEGDLPDTVKDDHKEALR
                     QLRELRDNRLTLGLPSTQKDVPEPGEFRVRSRPATFGGRDGLLEKY"
     gene            complement(5827..6162)
                     /locus_tag="PN977_003348"
     CDS             complement(5827..6162)
                     /locus_tag="PN977_003348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016191685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2190 family protein"
                     /protein_id="HDI6043352.1"
                     /translation="MTTQQVTLTMTVTASTALTQQRFVGADNGACKQGAVAVGVAQVD
                     ARAGDLTPVSVTGILAVETGAAVSRGALVQSDAKSRAVPQADSGKSCGIALDEAKAEG
                     DIIRILRGV"
     gene            complement(6173..7126)
                     /locus_tag="PN977_003349"
     CDS             complement(6173..7126)
                     /locus_tag="PN977_003349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020803522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6043353.1"
                     /translation="MSDYLKNKRVVDPVMTSIARGYRNAAFIGEHIFPVVYTDREAVT
                     VPVFGRSAFVEVDTVRAVGADSNVLIREKSSTLDLVLHEHDLAAPVDYREQTESMFNE
                     EAKAIRRVTNGIHLRRELIAARLAQDKKVYPASSVKTMTSVERWEGGHGDPVEVIEGG
                     IEAVRSKIGVRPNLMVMGASVMPLLRYHPAITTALGLNERKRITPQILRELFGLDKVI
                     VGEPVSMASMTDAGNRNKYPVDIWGDNLMLFYVTPPREGAESADENEPSFGYTLRRKG
                     MPVADKYDGVGGKVKYCRYTDIYKVAVVGGDAGYLVTNIVK"
     gene            complement(7136..8251)
                     /locus_tag="PN977_003350"
     CDS             complement(7136..8251)
                     /locus_tag="PN977_003350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001273074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HDI6043354.1"
                     /translation="MSAIHIFKAGTHTDMHGKKLPFTPDDLAACVKAYDPSVHEAPLV
                     IGHPRTEDPAWGWVKALSLSGVDLMAEPAQLDPQFAEMVTDGRFKKVSASFYLPDSPS
                     NPKPGVLYLRHVGFLGAQPPSVKGLKQVSFSEQEEGVVEFADWQAITNASLWGKLRDF
                     LIARFSLDEAEKVLPEWQLNSLREEAYRDTPSQDAAGAQFSETGQVPSSASNEESSMT
                     KEEIEALQAENRRLKQQAADRDARDAQVRQEQLHKDNVAFAEKLVAEGRLAPRASSVV
                     VALLDAVAGGDKPVEFAEGESRTPLATAFRSLLSDGEPVMNFAEQATKERVGDTVKVD
                     VTEFAEADPDRLALHQKAVELSKKEGISYEAAVTRCL"
     gene            complement(8466..8924)
                     /locus_tag="PN977_003351"
     CDS             complement(8466..8924)
                     /locus_tag="PN977_003351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000135514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage virion morphogenesis protein"
                     /protein_id="HDI6043355.1"
                     /translation="MASDNLVSVTINDKSLRRSLRALDLAATDMEPAMRKIAGTLLAE
                     TQFNFLDEGRPGWMPSLAAEERNGQTLQDTGRLMGSVSTEHDDRQAVVGTNVVYGAIH
                     QFGGKTGRNESVELPARPFLPVTEDGELQPEVVVPILDTIVRHLEAAARR"
     gene            complement(8927..9748)
                     /locus_tag="PN977_003352"
     CDS             complement(8927..9748)
                     /locus_tag="PN977_003352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016191689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
                     /protein_id="HDI6043356.1"
                     /translation="MSVTTAELAYCMTLPPKRAVSYLKSKGYQITWDQEEMWQDAHAR
                     AFTVAKVTRLDILEDIRGTLQQAVDEGKTDRWFRQELEPALKRRGWWGPRDTTDPVTG
                     EPVTIQQGSPWRLDTIFRTNMSVLYSAGRWAEQMENVDDRPYWMYTGINDSHTRRSHL
                     ALHGLVLRWDDPFWQAFYPPNGWRCRCSVIALSAADVRARGLKVISSGSAMGQELKLV
                     SEKTGEMWNVATFNTGTTKVTTDVGWSYAPGAAYRPDLARYQGTLQPLAQQELRG"
     gene            complement(9729..11225)
                     /locus_tag="PN977_003353"
     CDS             complement(9729..11225)
                     /locus_tag="PN977_003353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016240624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF935 domain-containing protein"
                     /protein_id="HDI6043357.1"
                     /translation="MAKGIWVSPDEFVAFAEPKKSLTAQIASRSRAIDFYALGMYLPN
                     PDPILKAQGRDIRIYRELRTDPLVGGCIRRRKAAVKSLERGLERGHASARVFRFIRDM
                     LDDLDLSRIIGEMSDAVLYGYQPCEIMWGRSVRAWAVTDIVGKPPEWFQFDTDNCLRF
                     RARDAGVEGELLSPSKFVVPAQDASYDNPYGFPDLSMCFWPVAFKKGGMKFWLRFAEK
                     FGSPWVIGKHPRGANDAEIEKLLDSMEQMVEDAVAAIPDDSSIELKAADGKADSSEVF
                     RELITLSRSEISIALLGQNQTTEANSNKASAQAGLEVTADIRDADADIIQAAVNQVIR
                     TVVTLNFGDVPCPVWAMWEQEAIDDTRATRDEKLTRAGLRLTPQYFKREYQLQDGDID
                     ETPPSECEKNMLPLSFAEAIDADIQAQQQLDDALDILMNGGVLNGTLEPVLAPLFRRV
                     ENGVNPTELLGELAELYPQMNTDDLQERLARILFVANIWGHLHERDNG"
     gene            complement(11225..12748)
                     /locus_tag="PN977_003354"
     CDS             complement(11225..12748)
                     /locus_tag="PN977_003354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6043358.1"
                     /translation="MTELPPHIPDTASYETPPVLLPYQQRWVADTSPLKVMEKSRRTG
                     ITWAEASDNVLTAASSAPAGGMNVYYIAYNQDMTVEYIQACAMWARAFNYAASEIEEG
                     FWEEDDDDKHIRTYTIKFPDSGFRIVALSSRPSNLRGRQGIIVIDEAAFHEQLDELLK
                     AALAMLIWGGKVRVISTHDGDDNPFSTLIGDIRAGRQGGSVHRITFREAVSEGLFQRV
                     CLRTGKEWSEASELAWMASVYKFYGAGASEELDCIPANGGGAWLSRALIESRMSADTP
                     VLRLTCKEGYELLSDEVRFHETQDWLDEHLKPLLEALPTDARSFLGRDFGRSGDLSVD
                     YPLLQEKNLVRRVPFVLELRNVPFRQQEQITWYLMDGLPGLLGAAFDARGNGAYLAEY
                     AMQRYGSSRVQQVMPTEGWYREHMPPVKAALEDGNLLDLPKDEDTLDDLRAVQVVNGV
                     PRVPEQRSKAKADGGKRHGDSAIALALAYFASREINKGPVKASSRRRRQAARMLEGF"
     gene            complement(12745..13287)
                     /locus_tag="PN977_003355"
     CDS             complement(12745..13287)
                     /locus_tag="PN977_003355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001696566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3486 family protein"
                     /protein_id="HDI6043359.1"
                     /translation="MGRRSSIDSLPKEVRRWLERALTENNFTGYAELESLLKEKGYSI
                     TRSSLQRFGYKMEQQLARVRAATEAARLLAREAGDDPDDRSAGLITLVQTEMTDILMR
                     LQESRENDDPFARAKLLATASKNIATLTRASVNLKRYQAEVRERVERAAAAAEKIARK
                     GGLSAEAVQALRREILGVAS"
     gene            complement(13290..13601)
                     /locus_tag="PN977_003356"
     CDS             complement(13290..13601)
                     /locus_tag="PN977_003356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000227551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6043360.1"
                     /translation="MVDITRVRRESLRWSLLVALNKTRPYTASETLLLEVSRAIYPDT
                     TQLELRRELDYLADRKMVELERRPSGDWFADLSRLGVDIVEYTVECGPGIARPEKYWS
                     E"
     gene            complement(13601..13927)
                     /locus_tag="PN977_003357"
     CDS             complement(13601..13927)
                     /locus_tag="PN977_003357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000175095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6043361.1"
                     /translation="MTLQVEFWTVVGFLITFMSFVGGMAKWLFSKAEERQAARFASLE
                     QSLQQSASSWGELEKELMRFKADLPLNYVRREDYIRGQTVIEAKLDALYNKLEVVQQY
                     RNTGGQ"
     gene            complement(13924..14577)
                     /locus_tag="PN977_003358"
     CDS             complement(13924..14577)
                     /locus_tag="PN977_003358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000264665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6043362.1"
                     /translation="MWTNLLKNLPWRSLLLAVLINAFLIGLYYLGYKSGHENATRDGD
                     KAISELQSAFDTYKTEQAMLENAALRAWAKRYQEQVAAGQQAEAGYLEQIAQLESQNK
                     QLQGQINDVTQRWIDEKGKSHPIECVFTRGFVRQYNAALGYDNASVDTGHSDSVAATG
                     TRSGTATGQPETTDTRLRDSGVSQRDVLANIIDNAGQCRRWRNQINALLDEREGLQK"
     gene            complement(14567..15301)
                     /locus_tag="PN977_003359"
     CDS             complement(14567..15301)
                     /locus_tag="PN977_003359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001104442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HDI6043363.1"
                     /translation="MNWPQSTWIVCMSLKLAFEAFLVFIRTERLSVRVGTFLVHMAWV
                     FFVAMLLWCGGFFSQARAAQPPQAALQYRDDVIRNARLEWGLSAPVADFAAQLHQESG
                     WRTDAVSPVGAQGLAQFMPATADWISQLMPALSSREPFNPAWAIRALVSYDRWLWQRI
                     SAASDCERMAMTLSGYNGGLGWVQRDRRLASQKGLDSTRWFGHVATVNAGRNAASWRE
                     NRHYPQRILHELAPRYLTWGGGSCVD"
     gene            complement(15304..15654)
                     /locus_tag="PN977_003360"
     CDS             complement(15304..15654)
                     /locus_tag="PN977_003360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011147630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative holin"
                     /protein_id="HDI6043364.1"
                     /translation="MKPFTSLIVDVQKTRLRGWLVAAVLLLLIIALVSPQQLPVVVYK
                     LSLISLAAVLGYWLDRSLFPKARPGQYLKHDERLMAEGRWPVQTGLHLVFSAALIRRA
                     LIVAAVCLAVAMGL"
     gene            15786..16334
                     /locus_tag="PN977_003361"
     CDS             15786..16334
                     /locus_tag="PN977_003361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000194950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6043365.1"
                     /translation="MTVTPKPTITVRVSGPVGSGKSYVLTRIKEMVKKEFGKSVIVDA
                     AVVDDERICRTNELLQEQLMKVLRKLTGQTTKCFSAPEYGIQEHIPVNMPPVNQPPRF
                     FDEYILTPENKAEHEEIKAALFREIRKIAEMQLEGGLDRVMARGEWESLFERVSPELL
                     PLALAIGIMQTADKPHLRSLNT"
     gene            16712..16927
                     /locus_tag="PN977_003362"
     CDS             16712..16927
                     /locus_tag="PN977_003362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001069608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6043366.1"
                     /translation="MNPRLTLTEHQRRAEAVNNVLEDIIRLHRGELSVCRATVHFQEI
                     QKQFDTSVFAEGITYALDQIRSENRPG"
     gene            17120..17884
                     /locus_tag="PN977_003363"
     CDS             17120..17884
                     /locus_tag="PN977_003363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000664222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA adenine methylase"
                     /protein_id="HDI6043367.1"
                     /translation="MKMQTLPIVPWIGGKRRLAKHILPLFPAHECYVEPFCGAAALYF
                     LKTPGKLEVINDINGELVNLYQVVKHHLEEFVRQFKWALVSRQIYKWLQITPTETLTD
                     IQRAARFYYLQKQAFGGKVAEHSFGTSTTSPPRFNLLRIEEELSAAHLRLSRTVIEHM
                     DWQQCIERYDRPHTLFYCDPPYLGTEGYGVDFPEGNYSRLAELARCIRGKMIISVNDI
                     PQMREVFTGLNIQTVNINYSLAGKSTPRRELVICNF"
     gene            complement(18003..18341)
                     /locus_tag="PN977_003364"
     CDS             complement(18003..18341)
                     /locus_tag="PN977_003364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000579778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDI6043368.1"
                     /translation="MIGLRIKEERERLSLTQQGLADAIGVAKRTFIDWEKDRTSPTAV
                     QLSALSEIGVDVLYVVTGVRSQPVVAPYMSQEKKELMDAFDEMSPEQRRAILEVGKVF
                     IQPKPSKLAG"
     gene            18442..18630
                     /locus_tag="PN977_003365"
     CDS             18442..18630
                     /locus_tag="PN977_003365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001510316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HDI6043369.1"
                     /translation="MTAEQVKALFRQRGITFTRWAEENGYNRNEVYRVLNGQSKARYG
                     KSHEIAVKLGLKSTAQAA"
     gene            18683..18991
                     /locus_tag="PN977_003366"
     CDS             18683..18991
                     /locus_tag="PN977_003366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015365656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDI6043370.1"
                     /translation="MSKTNVSSSGSRILRVLKALRGHALNGISNGELAAALHESPANI
                     NRALNTLIEEGLALKLENGRFAPGIQLLQIAMAHSNEMARAQDRINEINQRVISGSRL
                     "
     gene            19002..>19355
                     /locus_tag="PN977_003367"
     CDS             19002..>19355
                     /locus_tag="PN977_003367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001405162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3102 domain-containing protein"
                     /protein_id="HDI6043371.1"
                     /translation="MGRTKSPVNTELNAEVPLSDDLNVSLNAMTQHRMEIMQQFGDGL
                     PYERDRIVHEARFYMAQSAESMLEAGKRLIILKENEPHGDFVGIVEEQLGLHVRAAQR
                     MMKASLKYLSPKLEPK"
BASE COUNT         4354 a         5571 c         5102 g         4328 t
ORIGIN      
        1 aatgtcagcc cagcgctgtt taaaccagcc actgactgtc ccccagttgc ggtagataag
       61 gtaagctgct gccgcgacag cggtgataac gagaccgatg ggattcatca tcagcgctcg
      121 cccaatccag agaacggcac gtgcagcgct cataaaaccg cgaacgaggc tgcccgccag
      181 aactttgctg agtttcagtc ctccgctggt cagtcggcta aatccagtga caagccagcg
      241 taatttgcca ccttctccta gtgcggtagt cagtcgatgc cagttggtcc ggagtaaaac
      301 agcagttttc cagacgttca caaaggggga aataaggaga ttcagcccca gtttgagacc
      361 aacagtcgct atcttaatcg caaggagcgc actgatgagc ttaaaagccc cactgacaaa
      421 ttgcgggtga gtcgccaccc agtgttttgc cccctgaatg agtgggatca gctcctgagt
      481 cagagaaata aaagaggggg cgagctgatc gcccagcgta atagccagat cgcgactgct
      541 gaccataagc gctttagtgg cttcaagggg agatttcagc cgctgatcgt aagcactggc
      601 aagcaaatcg ttatccgcag ccctgagagc accggcacgg atctcgcgat atctgtccat
      661 gttggccagc atcgggcgga taaatgccat gacctgcata tccgcgaaca tatcgcccag
      721 accaaagttt ttcgccagag cctgaagtgc ctcatctctt gccgtatcat tctttatttt
      781 catggcggat ttgaagccgg ccagcgcttc ggggcttttg gcattgaggt aacgttctat
      841 aacactcaac atcccttcaa tcggagagat ccccgcagcc ttataactcg caacagaacc
      901 ctgcaaatca atacccagat cagcaaactg tttctgagta tcgcgggcaa aaattttggt
      961 aagaaagttt ttaaaattat tggctgcttc gtcggtggag cccgcaccga tttttgctat
     1021 ctggagactg gccccgattt cagcaacagc ctcttttcca ctggcaatac ccgccatcat
     1081 tggggccagg gactgcatcc acttgacctg atccgggatt tcaaatgacc cctggtcacc
     1141 ggcataagcc ataatattct ggacggcact aaaatctctg gccgcacctt tcagggagtt
     1201 ttgccagact gcggccactt ttgcccagtc ctgagatgac gtgcgtgttg ccgttgccgc
     1261 gcgggcaata tccggcatca gaaaaccgat atctgacaag ttatcaatat tgtcgctgat
     1321 gagcgaaccc accgcctcct gcagttcatc ctgatactga ttgtattttt gcgcccagcc
     1381 ttttatctgt ccggcaagtg catcccgcgt tttattgtca tattttgcgg tgatcgacat
     1441 atcaatcatt ttgtcctgaa aagacatgga ctgctgaacg gcaggggaaa ccgtatgata
     1501 aacagtctgt gccatgccat acgcttcaat cccctgacca tacagcgcca tgcggttagc
     1561 tttcagcgca tcactggtgg ctgatgccgc tgacagacgg cgctgctggc gctcaatttg
     1621 ctccatggtg cggcttaccc gcagcagctc gctgttgaga tgctgcatcc gggaagagcc
     1681 cagttgacca taacgttctg ttgcacgggt taaagcgttc tgacgttcct gcaggcggcg
     1741 tgaggtatcg ctcagggaat caagcgcacg gcgggtaccg ctcattgcag aacggaatgt
     1801 gctgccaatc atcccgccga taacaacgcc gactgaaaac tgtcccgaca tagtggttaa
     1861 cctccgggga aggtgaaaag acgtgagggg ataacgcaga acagccgcta ctggcggctg
     1921 tctgtactat gatttgtcgc cgtactcgct ttttatttgc tcttcagcct gctccagcca
     1981 catctccaga tcgtcagtat cgagggcatc aatctccccc ggctgaaacc ggaaccatct
     2041 cgccagcagc ccctgcgcct gcgtcagcgc ctttgtcgct ctcacccatc cccgtgatga
     2101 gctgaaatcg tttctgtaac tgcaggtaat cagccagatc catattgtcg agatcttccg
     2161 ggagaagacc agtactgcgg gcaatcagcg gttcgtccca gtctgccggg tttttgctga
     2221 ttttgcgcac ctgcttcagg tcttttaccg tcaggcgttt cagttcaacc agctcaattc
     2281 tggtgcctgc agcagtggtg aagggataag acaatttaaa agtatcggat ggggtctgtg
     2341 acatgattgt gctcctgtgt aagttcaggg cagtatgtcg ggagaagcgc gtgacggata
     2401 ttaaaggaga ttaagaagaa ggggccggag cccctgtgat gtcagcaagt gcgaaacccc
     2461 ttgcagttac gcaggaaagc gatgagaagc gtctttccct cagatttgcc ggtgccggag
     2521 aaccagtggt cgggaggctc ccatgcttca atcaaatccg ccagtctgcg ggcctttgaa
     2581 cgtgtgcagt caatcgggtc attggtttta cgggtattaa aaagggtttc cacccccgga
     2641 atatcaagga gggtaaacca cgtaccattt gacatgccca gtgccgcaca tcgtcctcct
     2701 ttatccgtca gttcaacact caccgtcagc ccccgatatt gatacggtag tcagtcagtt
     2761 gatcaacacc gccgacccgg aagatgttgg ccagatagtc cagttgcagc agctcttcac
     2821 catccagtac ctgtctgata tacgtgcatg tgaagctact ggagaactcg gcgttctcgt
     2881 gctgtttgaa cgtccccagc gggttcttct tgaacataat cgtcaggaag gtgaccagcg
     2941 ggatttcgtc aatcagcccc tgcgagctgt agcgctggac gctggaacga cactgcagtg
     3001 ccagcgacct gtacgggttc gcggcagaca gcatcgcatc gcggtaaaag ctgttccatt
     3061 tgatttcgcc ttccagtttg tcaaaaccag ccgggagttc caccttaccc accatcccca
     3121 gcgccttgtg ttcctgcata atcatggaga catcggggag tttaacttcc tcagcccgtc
     3181 ccagcaggtt agtaccatcc agatagatgt tggcattcgt gatgcggttt atctcaatct
     3241 ttgacatcag ttgccccctt tcagggttaa caggtattcc gaggtgatct cagtctcaaa
     3301 cgtcagtcgc tccagcggcg gtggtggcgt atatttgtag ctcagcaaca ggtgcccggc
     3361 ggccagctcc gtctccggat tgcgggccgg atcaaaccag caacggaagc ccagtaccgc
     3421 accatcacca gtcattttgc gaccgtaggc gttgaccgac tccgtcagtg catcaatcag
     3481 cgcctgagta atcggcatgt cgatgtactg ctggctgaaa taacgaatgg actcgttgat
     3541 cacatcaccg gtgcggcgaa cgttctcaaa gttacgcata tgggtgaccg ttggccatgc
     3601 tgccgtccgg ttaccccaca gacgaaggcc gctgccgtaa ctgctgaata ccgtggtgat
     3661 cccctgttcg ttaagcaggt tcacctcact ctgcgggtca tcaatcatcg cggacagctg
     3721 gcgctccacg ccggtgatcc ccagaatctc ctgattggag gatgaccacc agtagccctt
     3781 gtccagatcg actctggcac gcagacctgc tgcacgctgg ctgagcggct ccagacgctc
     3841 cgtgtttgtc accgggtcat ataccttcac atgcggatag cacagacgga cgcggtcgga
     3901 gctggtattg aagttaatgg tgccttccgg gccacgacct gccagagcct gtgcaaaggt
     3961 ggtaccgaca ggcgcatcaa tgtaggttac cgcgcccagc ttctctgtca tggcgataag
     4021 ctcaactgct acactcttct gggtgcagaa caccggcgca atcagaattt tggcaaaata
     4081 gccgtacagg ttgaagctgt cgttaagcag cttcatgccg gttcggtttc cggcgctgtt
     4141 caccccgccg atgatgtccg ccgcagtcac cttcgacggg tccgcgtact catagctcac
     4201 cttcacgctg ccaccggcct caatagcctt gcccaggttc gtgagcacac ccgcctgcgc
     4261 atcaacacgg tagtccgtgt tcgccgtgtg ggtggtgctg ccttcactgt ttttcaccac
     4321 cacattagcg acaaccggat gcgcaagtct ggcctgccct gtcgatttgt caaaggtaac
     4381 cacttcaccc tggatcgccg ttttatgttt cgccgggtcg aggacgttaa tgaccagaac
     4441 ggtgcctgca ccatggtcat aaatcgcatc cagcgcctgc ggaatggtaa agccggtgaa
     4501 ctggctgcca aatgccgctg cgtctttctc agacaggcac tgtaccagcg tattgacgtc
     4561 ccccatcggg gcggtaccaa tcaggccaat aacggcagat ttcaccgttt taaccgggcg
     4621 ggcaccgttt tccacctcaa tggtttcggg accatgcaga tagttagctg ccatgggtgt
     4681 cctccgtttt cacttcgctg tcactgctgc ttctgcgctt tggtgactgc acagccggtg
     4741 tgccggcgag tttagtctct tcaggtaccg gcgtcagatg tttcagcgcc accagtacct
     4801 tcacgtagtc atgctcctcc ggcagggaaa acgtcttccc cggccagagc aggatttcgg
     4861 ttccgtccga cagcgtgacg ccgctggccg ggccggaata gcggtatgct ttcatcactc
     4921 gctttcctca tagttcactt cggttaacag cgggccggac ggtaaatcgc tgtcttcgat
     4981 aaagacgctt tcagtcgcga agtcgagggc gtattgccac agccccctga cttcaccgat
     5041 aaacacctcg cgggtcagcc agatacgacg gcggcagccg ggcggggtgt agccaccgag
     5101 aatgcggcgg acagcatcca ggacattaat cgcccctttt ttaccgttga gctggcggaa
     5161 gaccaccgtg acgcagagct ggatagtctg aggctggatc accgcaccgg tatcatccgg
     5221 cctgtcaaag cgcgaaccgg catagctcag cagcaacgcg ccaaccggat ggttcaggcg
     5281 atattcagcc ggtttctccg ggaagtactc cacctgcagt tgcggcaggt tctcgcgtaa
     5341 ccgggccagt accgcatcaa ggacgggcag aacgttcatc agtatttctc cagtaaaccg
     5401 tcacgaccgc cgaaagtggc cgggcgactg cgtacacgaa actctcctgg ctcaggcaca
     5461 tctttctgag tggacggcag ccccagcgtg agcctgttat cacgtaactc cctgagttgc
     5521 cgcagcgctt ctttgtggtc atccttcacc gtatccggga ggtcaccttc cgggcggcgg
     5581 gcgtagagcc ggtaacggac cagcgtgatg gcaatgtccc gcagaacggt cggtatctcc
     5641 gccagcggca ggatatagcg tccgcgcaga tgggcatcaa tcagctcgtc ggcatagcgg
     5701 atacagctgt ccaccacacg ggtattcact gttgcaggcg agtcgaagtc catatcttca
     5761 ctggtgagct cgatcagcgt tcgttccggc acctgcgcaa gcaaatcctc cagggtgcag
     5821 tacatctcac accccgcgca ggatacggat gatgtcgcct tcggcctttg cctcatccag
     5881 tgcaatgcca caggatttac cactgtcagc ctgaggaaca gcccttgatt tggcatcaga
     5941 ctgtaccagt gcccctctgg aaactgccgc tcccgtttca actgcaagga tcccggtcac
     6001 actcaccggg gtcagatcac ctgcacgggc atccacctgg gcgacaccga cggcaacggc
     6061 accctgctta catgcgccgt tatctgctcc cacaaacctc tgctgagtaa gtgcagtact
     6121 ggctgtgact gtcatggtca gagtgacctg ctgtgtagtc atagacttct ccttacttca
     6181 ctatatttgt aacgagataa ccggcatccc cgcccacgac cgccacctta tagatgtcgg
     6241 tataacggca gtacttcacc tttccgccca ctccatcgta tttatctgcc accggcatcc
     6301 ccttacgtcg cagggtatac ccgaacgatg gttcattttc gtcggcactt tcggcacctt
     6361 ctcgcggtgg agtgacatag aagagcatga gattatctcc ccagatgtcc accggatatt
     6421 tgttccggtt gcctgcatca gtcatggatg ccatggaaac aggctcaccc acaatgacct
     6481 tgtccagacc aaaaagctcc ctgaggattt gcggggtgat ccgttttctc tcattaagtc
     6541 ccagagcagt ggtgatggct ggatgatatc tcagcagggg catcacactg gctcccatga
     6601 ccatcagatt gggtctgacc ccgatcttac ttcgcacagc ttcaatgcct ccctcaatga
     6661 cctcaacagg atcaccatgg cctccttccc agcgttcaac agacgtcatc gtctttaccg
     6721 aagatgccgg gtacactttt ttgtcctgcg caagacgggc agcaataagc tcacggcgaa
     6781 gatgaatgcc atttgtcacc cgccggattg ccttggcctc ttcgttgaac attgactccg
     6841 tctgttcacg gtagtccaca ggtgcagcaa gatcgtgctc atgcaacacc agatccagcg
     6901 tactgctctt ttccctgatc agaacattac tgtctgcccc cacagcacgg acggtatcaa
     6961 cttccacaaa cgctgatctc ccgaaaacgg ggaccgttac cgcttctctg tccgtataca
     7021 caaccggaaa tatatgttct ccgatgaagg ctgcattcct gtagccacgc gcgatgctgg
     7081 tcataacagg atcaacaacg cgtttatttt ttaagtaatc agacatgctt tctccttaca
     7141 ggcagcgtgt gacagcagcc tcatagctga tgccttcttt tttcgacagt tccacagcct
     7201 tctggtgcag tgccagacgg tcagggtcgg cttctgcaaa ctccgtcaca tccactttca
     7261 ccgtgtcgcc gacacgctcc ttcgtggcct gttcggcgaa gttcatcacc ggctccccgt
     7321 cggagagcag cgaacggaag gcggtggcca gcggtgtgcg gctttccccc tcagcaaact
     7381 ccaccggctt gtcgccaccg gcgacggcat ccagcagggc aaccaccacg gaggaggcgc
     7441 ggggagccag acggccctct gcgaccagtt tttctgcaaa ggccacattg tccttatgca
     7501 gttgctcctg tctgacctgt gcatcacgcg catcgcgatc agcagcctgc tgcttcaggc
     7561 ggcggttctc cgcctgaagg gcttcaatct cttcttttgt catcgatgat tcctcgttac
     7621 ttgcggaaga cggcacctgg cctgtctcac tgaattgcgc acctgctgca tcctgcgacg
     7681 gtgtgtcgcg gtacgcctct tcgcgcagac tgttgagctg ccattccggc agaacttttt
     7741 ctgcttcgtc cagactgaag cgggcgatca gaaaatcgcg cagctttccc cacagtgagg
     7801 cattcgtgat ggcctgccag tcggcgaact ccaccacacc ttcttcctgc tcactgaagg
     7861 acacctgttt cagtcctttg acggaaggtg gctgtgcccc aagaaagccc acatggcgca
     7921 ggtagagcac gccgggcttc ggattggacg gtgaatccgg gaggtagaaa gaggcagaca
     7981 cttttttgaa tcgtccgtcg gtgaccatct cagcaaactg cgggtccagc tgggcaggct
     8041 ctgccatcag atcgacgccg ctgagcgaca gggctttcac ccagccccac gccgggtctt
     8101 ccgttctggg atgaccaatc acgagtggtg cttcatggac ggacgggtca taggctttca
     8161 cgcaggcggc aagatcgtct ggcgtgaacg gcagtttttt gccgtgcata tcggtatgag
     8221 taccggcttt aaaaatgtga atggctgaca ttttgctgtc ccgcgttatg ttgtcggaga
     8281 cagtttgtga gaaatgcggg cccggcgatt ttaatctgct ttagaaaaca tcaggggaga
     8341 aggacaggga aagcaatgtg gtgaaccgga ggcagttata aaacagaggc tgtaaagcct
     8401 ttataaaggt aatacagccc ctcattcgct ggcaatgata aatcacccgc ctgaagagag
     8461 aaaactcagc gacgggccgc tgcttcaaga tggcggacaa tggtatcgag gatggggact
     8521 accacttcag gctgtagctc tccatcctcc gtcaccggca ggaacgggcg ggccggaagt
     8581 tcaacagact cattacgccc cgttttaccc ccgaactggt gaatggcacc gtaaacaacg
     8641 ttggtcccca caacagcctg ccggtcgtca tgctcggttg atactgaccc catcagacgc
     8701 ccggtatcct gcagtgtctg tccgttacgt tcttccgctg ccagcgaggg catccacccc
     8761 ggacgcccct catcaagaaa gttaaactgt gtttccgcca gcagggttcc ggcgattttg
     8821 cgcatcgcag gctccatgtc tgtggcagcc agatccagcg cacggaggct ccggcgcagg
     8881 gatttatcat taatggtgac actgaccagg ttatcggaag ccattgttat cctctcagtt
     8941 cctgttgtgc cagtggctga agcgtaccct gatagcgggc caggtcggga cggtatgctg
     9001 cccccggtgc ataagaccag ccgacgtcgg tggtcacctt cgtggtgccg gtattaaagg
     9061 tggccacgtt ccacatttcg ccggttttct ctgagaccag tttcagttcc tggcccatgg
     9121 cagagccgga gctgataacc ttcaggccac gggcacgtac atccgccgca ctcagggcaa
     9181 tcacactaca gcggcagcgc cagccgttcg gcgggtaaaa tgcctgccag aacgggtcat
     9241 cccagcgcag caccagacca tgcagcgcca gatggctcct gcgggtatgg ctgtcgttga
     9301 tgccggtata catccagtac ggcctgtcgt cgacgttttc catctgctcc gcccaacgac
     9361 cggcgctgta gagtacggac atattggtgc gaaagatggt atcgagccgc cacggactgc
     9421 cctgctgaat ggtgaccggc tcacccgtta ccgggtcagt cgtgtcacgc ggtccccacc
     9481 atcccctgcg cttcagcgcc ggctccagct cctgccggaa ccagcgatcg gtttttcctt
     9541 catcaacagc ctgctgcagt gtcccgcgaa tatcttccag aatatccagg cgggtcactt
     9601 tagcgacggt aaaggcgcgg gcatgggcgt cctgccacat ttcttcctgg tcccaggtaa
     9661 tctgataccc tttggacttc aggtagctga ctgcccgctt cgggggaagc gtcatgcagt
     9721 acgccagttc agccgttgtc acgctcatgc agatgccccc agatatttgc cacaaagaga
     9781 atacgggcca gccgctcctg cagatcgtcc gtgttcatct gagggtagag ctccgccagt
     9841 tcgcccagca gctcagtcgg gttaaccccg ttttcgaccc gtctgaacag aggtgccagg
     9901 acgggttcca gcgtgccatt taacacacct ccgttcatca gaatgtccag cgcgtcgtca
     9961 agctgctgct gagcctgaat atcggcatca atcgcctcgg caaatgacag cggcagcatg
    10021 ttcttctcgc attctgacgg tggtgtctcg tcaatatcgc cgtcctgcag ctggtactca
    10081 cgcttgaagt attgcggggt cagacgcaga cccgcccggg tgagtttttc atcgcgggtg
    10141 gcacgggtgt catcaatggc ctcctgttcc cacatggccc agaccggaca cggcacatcg
    10201 ccgaagttca gggtgaccac cgttcttatg acctgattca ctgccgcctg aatgatgtcc
    10261 gcatccgcat cgcggatatc agccgtcacc tccagcccgg cctgtgcaga ggccttgtta
    10321 ctgttcgctt ccgtggtctg attctgaccg agtaatgcaa tggagatctc actgcgtgac
    10381 agtgtgatca gctcgcggaa tacctcgctg ctgtccgcct tgccatccgc tgctttgagt
    10441 tcgatgctgc tgtcatcagg gatggcggcc accgcgtcct ccaccatctg ctccatggag
    10501 tccagcagtt tttcaatctc tgcatcattt gcacccctcg ggtgcttacc gatgacccag
    10561 ggggagccaa acttttcggc aaagcggagc cagaatttca tcccgccttt cttgaaggcg
    10621 accggccaga agcacatgga caggtccggg aaaccgtaag gattgtcata cgaggcatcc
    10681 tgtgccggca ccacgaattt tgacggtgac agcagctcac cctccacacc cgcatcacga
    10741 gcccggaagc gcaggcagtt gtccgtatca aactgaaacc actcaggcgg tttgccgaca
    10801 atatccgtca ccgcccacgc cctgaccgaa cggccccaca tgatttcaca gggctgatac
    10861 ccgtagagca cggcatcgct catctcaccg atgatgcggg acagatccag atcgtcgagc
    10921 atgtcgcgga tgaaacggaa gacccgggca gaagcgtgac cgcgctccag tccacgttcc
    10981 agcgatttga ccgctgcttt acgtctgcgg atacagcccc cgaccagcgg gtcggtgcgc
    11041 agttcgcggt agatacggat atcccgtccc tgagccttga gaatgggatc agggttgggc
    11101 aggtacattc ccagagcgta aaagtcgatg gcgcggctgc gggaggcaat ctgcgcggtc
    11161 agtgactttt tgggttcggc aaacgccacg aactcatcgg gtgaaaccca gatacctttt
    11221 gccatcagaa tccctccagc atacgggccg cctgacgacg acgtcgtgag cttgccttca
    11281 ccggcccttt gttaatttca cggctggcga aatacgccag cgccagtgcg atggctgaat
    11341 ccccgtggcg tttaccacca tcagcctttg cttttgagcg ttgttccggc acgcggggga
    11401 caccgttcac cacctgaacg gcccgcaggt catccagtgt gtcttcatct tttggtaagt
    11461 ccagcaggtt accgtcttcc agtgcagctt tgaccggagg catatgctcc cggtaccagc
    11521 cttcggttgg catcacctgc tgaacccggc tggagccgta gcgctgcatg gcgtattcag
    11581 ccagataggc accattacca cgggcatcaa atgctgcacc cagcagaccg ggcaggccat
    11641 ccatcagata ccaggtgatt tgctcctgct gtctgaacgg cacgttacgc agctccagta
    11701 cgaatggcac gcgtcgtacc aggttcttct cctgcagcag gggatagtcc accgacaaat
    11761 caccgctgcg accaaagtcg cgccccagga aagagcgggc atcagtgggg agtgcctcca
    11821 gcaagggttt cagatgctca tcaagccagt cctgtgtctc gtggaagcga acctcatcag
    11881 acagcagttc ataaccttcc ttgcaggtca gacgcaatac tggcgtatca gcggacattc
    11941 gggactctat cagggcacgg gacagccagg caccgccacc gttggccgga atacagtcaa
    12001 gctcttcgga tgcgccggca ccgtagaatt tgtacaccga cgccatccag gccagctcgg
    12061 atgcctccga ccattccttc ccggtgcgca gacaaacgcg ctggaacagc ccctcagata
    12121 cggcttcccg gaaagtgatg cgatgtacgc tgcctccctg acgtccggca cggatatctc
    12181 cgataagcgt actgaatgga ttgtcgtcac cgtcatgggt ggagataacg cgtaccttgc
    12241 ccccccagat aagcatcgcc agcgccgctt tcagcagttc gtccagttgc tcatggaacg
    12301 ccgcttcgtc gataacaata ataccctgac ggccacgcag gttagacggg cggctggaga
    12361 gcgcgacaat gcgaaagccg gagtcaggaa atttgatggt gtaagtcctg atgtgtttat
    12421 cgtcgtcgtc ctcctcccag aatccttctt caatttcact ggccgcatag ttgaatgccc
    12481 gcgcccacat cgcacacgcc tgaatgtatt cgacggtcat atcctggtta taggcgatgt
    12541 aatacacatt catcccgcct gcaggcgcag aagaggctgc ggtcagtacg ttatcggatg
    12601 cctcagccca tgtaataccg gtacgacggc tcttttccat caccttaagc ggagaggtat
    12661 ctgccaccca gcgctgctgg taaggcaaca gaaccggcgg tgtctcataa ctcgcggtgt
    12721 caggaatgtg cggcggcagc tccgtcatga cgccaccccc agaatttccc ggcgcagtgc
    12781 ctgtacggct tccgcagaga gcccgccctt acgggcaatt ttctcggcgg cagcggcagc
    12841 acgttcaacc ctctctctga cttctgcctg atagcgcttg aggttgaccg aggcgcgggt
    12901 cagtgtggcg atattttttg aagccgttgc cagcagtttt gcccgggcga agggatcgtc
    12961 gttttcccgc gactcctgca ggcgcatcag gatatccgtc atttcggtct ggacgagggt
    13021 tatcagtccg gctgaacggt catcaggatc gtctccggcc tcccgggcaa gcagacgcgc
    13081 tgcctcggtt gccgcccgca cacgggccag ttgctgctcc attttgtagc caaaccgctg
    13141 cagggaagag cgggtgatgc tgtacccctt ttccttcaac agactttcaa gctctgcata
    13201 gccggtgaaa ttattctccg tcagtgcccg ctcaagccag cgacggacct ccttcggcag
    13261 agaatcaatg ctgctgcgac gtcccataat cactcactcc agtatttttc cgggcgggca
    13321 ataccggggc cgcattccac ggtgtattcc acaatatcca caccaaggcg actcagatcg
    13381 gcaaaccagt caccggacgg tctcctctcc agttccacca ttttgcgatc tgccaggtaa
    13441 tcaagctcac ggcgcagttc cagttgcgtg gtgtccgggt agatggcgcg ggacacttcc
    13501 agcagcagcg tctcgctggc ggtgtaaggg cgggttttgt taagggcaac cagcagactc
    13561 cagcgcaggg attcacggcg tacccgggta atatcgacca ttattgacct ccggtattgc
    13621 ggtactgctg taccacttcc agtttgttgt aaagcgcgtc cagtttggcc tcgatgactg
    13681 tctggccacg gatataatcc tcgcgacgga cataattcag cggtaaatcc gctttaaatc
    13741 gcatcaattc tttttccagc tcgccccaac tggaggcgga ctgctgcagt gactgctcaa
    13801 gagaggcaaa acgtgcagcc tgacgttcct ccgctttact gaacagccat ttggccatac
    13861 cgccaacaaa gctcatgaag gtgatgagaa agcccaccac cgtccagaat tcaacctgca
    13921 gtgtcatttc tgtaatcctt cccgttcatc cagcaacgcg tttatctggt tccgccagcg
    13981 acgacattgt cctgcgttgt cgatgatgtt ggcgagaacg tcacgctggg agacacccga
    14041 atcgcgtaac cgggtgtcag tggtttcagg ttgcccggtc gctgtgccag agcgggtgcc
    14101 agtggcggca actgagtctg aatgaccggt gtcgacggat gcgttgtcat acccgagtgc
    14161 ggcgttgtac tggcgcacga aaccgcgagt aaacacgcac tcaatgggat ggctcttacc
    14221 tttttcatca atccagcgct gtgtgacatc gttaatttgc ccctgtagtt gtttgttctg
    14281 actctccagt tgagcaatct gctcaagata accggcctca gcctgctgcc cagcggccac
    14341 ctgctcctga taccgttttg cccatgctcg caatgcagcg ttctcaagca ttgcctgctc
    14401 cgttttgtat gtgtcgaatg ctgactgcaa ctcactgatc gccttatcgc cgtcgcgcgt
    14461 ggcgttttca tgcccgcttt tgtagcccag gtaataaagt ccaatcagaa aagcatttat
    14521 gagcactgcc agcagcagac tgcgccacgg cagatttttt agcaggttag tccacacagc
    14581 tgccgccccc ccatgtcaga taacgggggg ccagttcgtg caggatgcgc tgcggataat
    14641 ggcggttctc ccgccagctg gcagcattgc gtccggcatt caccgtggcg acatgtccga
    14701 accagcgggt gctgtccaga cctttctgtg atgcgagccg cctgtcccgt tgtacccaac
    14761 ccagaccacc gttatagccc gacagtgtca tggccatacg ctcgcagtca ctggcggcgc
    14821 tgatgcgctg ccacagccag cggtcatagc tgaccagcgc ccggatggcc catgccggat
    14881 taaacggctc acgactgctc aacgccggca tcaactggct tatccagtca gcggtggcgg
    14941 gcataaactg cgccagcccc tgagcgccca ccggcgagac cgcatcagtc cgccagccgc
    15001 tttcctgatg cagttgcgcg gcgaaatcgg ccaccggcgc agacagcccc cattcaagcc
    15061 gggcattacg gatcacatca tcgcgatact gcagcgcagc ctgcggaggc tgcgctgcgc
    15121 gggcctgact gaagaagccg ccacaccaga gcaacattgc gacaaagaat acccatgcca
    15181 tatgaaccag aaaggtcccg acacggactg acaggcgttc ggttctgatg aagaccagaa
    15241 aggcttcgaa agccagtttc agggacatgc atacaatcca ggtgctctgt ggccagttca
    15301 tgattaaagc cccatcgcaa cagccagaca gacagctgca acaatcagtg cacggcggat
    15361 taacgcagca gaaaacacca ggtgaaggcc agtctgtacg ggccagcgcc cctcagccat
    15421 gaggcgctcg tcatgcttca ggtactgccc cggacgggcc ttcgggaaga gtgagcggtc
    15481 cagccagtag ccgagcacgg cggcgaggga gatgagagag agcttgtaca ccaccaccgg
    15541 caactgctgg ggtgagacca gcgcgatgat gaggagcagc agcacggccg caacaagcca
    15601 tccacgcagg cgggtttttt gtacgtcaac gataagggaa gtgaatggtt tcatgaaaat
    15661 gtctccttgt tgtgtggaga cagcatggca aaagcgtggc gcggaggatt ttaaagcgcg
    15721 ttaagagaga ggcgtgttcg gggtatgcag aatacacgct ggatcactca caaaggagat
    15781 acagaatgac agtcacaccg aagccgacca tcaccgtcag agtgtccggg cctgtaggca
    15841 gcggtaaaag ttacgttctg acgcgtataa aagagatggt gaaaaaggaa tttggaaaaa
    15901 gcgtcattgt cgatgccgca gttgtggacg acgagcgtat ctgtcggacg aatgagctgc
    15961 ttcaggagca actcatgaag gtgttacgca aactcaccgg tcagacaaca aaatgttttt
    16021 cagccccgga gtacgggata caggaacaca tcccggtgaa catgcccccc gttaaccagc
    16081 ctccccggtt cttcgacgaa tacattctga caccggaaaa taaagctgag catgaagaga
    16141 tcaaagcggc gttgttccgg gaaatcagga agattgctga aatgcagctg gagggcgggc
    16201 tggacagagt gatggcgaga ggagaatggg aatcgctgtt tgaaagagtt tccccggaat
    16261 tgcttcccct cgcactggct atcgggatta tgcagacagc ggataaacct cacttacgtt
    16321 cattaaacac gtaacgcata atccgggata cgcattcagc gatgactgca ttttcaccgg
    16381 tttttactgc atccagaagt gactctttaa atggtacggg ccggggctgc agaaaggtcg
    16441 gcatggcatt gagcagacag agcgtttttt ccgtgaggac gacatcagcc gctcccacag
    16501 ccagcaactg agagtaacga atatagctgt tatcccgaag ccatatcagc gtatcaataa
    16561 aataccggca ctttttctgt cgtgatcaac ctggtccggg atatcgaaaa aaccagaata
    16621 aatcaaaacc tgaggagccg gatgcgtgtt cagtaatcgg cagaatattt tgccggtgag
    16681 tacataaaaa tcatcattta aggagtgcat catgaatccc cgattaacgt taacagaaca
    16741 tcagcgccgt gccgaagcag tgaacaatgt tctggaagat attatccggt tacatcgcgg
    16801 agaactgagt gtctgtcgtg ccactgttca ttttcaggaa atacaaaagc agtttgatac
    16861 ttctgttttt gcagaaggga tcacttatgc cctggaccag ataaggtcag aaaatcgccc
    16921 cggataatcc ctaaaccaca ttaacagaaa tcacttcatc cctgccgcat catgaaagaa
    16981 cgaccagccc ggtgcttcgg acccaccggg ctggccatcg tcccacagga atgcgctgtg
    17041 agccgaccgg ggttcagtca gtctcgcgag accagactag cctgccatat ttttaataat
    17101 tgtaaaaggc ttacagatta tgaaaatgca gacattaccc atcgttccat ggattggtgg
    17161 caaacgtcgc ctggcaaaac acatcctgcc gctgtttccg gcgcatgaat gttacgtgga
    17221 gccgttctgc ggcgcagcag cgctctattt tcttaagaca cctggtaagc tcgaagtcat
    17281 taacgatatc aatggtgaac tggtaaatct gtatcaggtg gtaaaacatc atctggaaga
    17341 atttgtccgc cagttcaaat gggcgctggt cagccggcag atctacaaat ggctgcagat
    17401 caccccgaca gaaacactga cggatatcca gcgtgcagca cggttctact acctccagaa
    17461 gcaggcgttt ggcggcaagg tggcagagca cagcttcggc acctccacca catccccgcc
    17521 acgcttcaac ctgctgcgta tcgaggaaga actgtcagcg gcacacctgc ggctttccag
    17581 aacggtgata gaacacatgg actggcaaca gtgcattgag cgctatgacc gtccgcacac
    17641 gctgttctac tgtgacccgc cgtatctggg tacagaaggc tatggtgtgg attttcctga
    17701 agggaactac tcgcggctgg cagagctggc ccggtgcatc agaggaaaga tgataatttc
    17761 ggtgaacgac atcccgcaga tgcgcgaggt gttcactgga ctgaacatac agacagtgaa
    17821 catcaactac agtctggcag gcaaatcaac tccacgccgt gagctggtga tctgcaattt
    17881 ctaaaagcca taaccggaaa gtacagtgct ttccggtaag cccgccgatt tctgccatgc
    17941 tactgacggc gttttctgaa agcatccaga tcaaccacct gtcctttctg tccaggtcag
    18001 aatcagcctg cgagtttgct tggtttgggt tgaatgaaga ctttgccgac ctcaagaatt
    18061 gccctccgtt gttcggggct catctcgtcg aatgcatcca ttaactcttt cttttcttgt
    18121 gacatataag gtgcaacaac aggctgactc cgcaccccag taactacata caaaacatct
    18181 acaccgattt ctgatagtgc agataactgc actgcagtag gagaagtgcg atctttttcc
    18241 caatcaataa aagttctctt tgccacaccg atagcatcag ccaacccttg ctgtgtaaga
    18301 gatagacgct ctctttcttc tttaatacgt aacccgatca tgtgagtttt tctgcactct
    18361 ttaagttgac tggtgagata atctgcacca taatctatca cacataaggc aaacatcatt
    18421 gcatcaacaa aggagacaac gatgactgca gaacaagtca aagctctctt ccgccagcgc
    18481 gggatcactt tcacccgctg ggcagaagaa aacggctaca accgcaatga ggtataccgc
    18541 gttctcaatg ggcagagcaa agcacgttac ggtaaatccc atgaaatcgc cgtgaaactg
    18601 gggctgaaat caacagctca ggcagcctga atattttgat tctttgtaaa aggttatcac
    18661 atatcgcaaa agaggtatgc agatgagtaa gacgaatgtt tcaagctccg gcagtcgcat
    18721 cctccgggta ctcaaagcgc tgcgcggtca tgccctgaac ggtatttcca atggtgaact
    18781 ggcggcagca ctgcacgagt caccggcaaa catcaaccgg gcactcaaca ccctcattga
    18841 agaaggactg gccctgaaac tggaaaacgg gcgtttcgca ccgggcatcc agttgctgca
    18901 gatcgccatg gcccacagta acgagatggc acgtgcacag gatcgcatta acgaaatcaa
    18961 ccaacgtgtc atttcaggta gtcgtttgta aggagtaatc aatgggacgc accaaatcac
    19021 cggttaacac tgaactgaac gccgaggtac cgctgtcgga tgatctcaac gtcagcctga
    19081 atgccatgac acagcatcgc atggagatca tgcagcagtt tggtgatgga ctgccttatg
    19141 aacgtgatcg catcgttcac gaagcacgtt tttatatggc gcagagtgct gaatctatgc
    19201 tggaagcggg taaacggctg atcatcttaa aagaaaatga gccacacggg gactttgttg
    19261 gaattgtaga agaacaactt ggtttgcacg tgcgcgcagc acaacgaatg atgaaagcat
    19321 ctttgaaata cctttcacca aaactagagc caaaa
//
LOCUS       DAMZFT010000091        18576 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.091, whole genome shotgun sequence.
ACCESSION   DAMZFT010000091 DAMZFT010000000
VERSION     DAMZFT010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18576)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18576)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18576
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.091"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            208..315
                     /locus_tag="PN977_003368"
     CDS             208..315
                     /locus_tag="PN977_003368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HDI6043372.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASLIVNSLNKRK"
     gene            complement(402..2081)
                     /gene="bcsG"
                     /locus_tag="PN977_003369"
     CDS             complement(402..2081)
                     /gene="bcsG"
                     /locus_tag="PN977_003369"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsG"
                     /protein_id="HDI6043373.1"
                     /translation="MTQFTQNTAMPSSLWQYWRGLSGWNFYFLVKFGLLWAGYLNFHP
                     LLNLVFAAFLLMPIPRYSLHRLRHWIALPIGFALFWHDTWLPGPESIMSQGSQVAGFS
                     TDYLIDLVTRFINWQMIGAIFVLLVAWLFLSQWIRITVFVVAILLWLNVLTLAGPSFS
                     LWPAGQPTTTVTTTGGNAAATVAATGGAPVVGDMPAQTAPPTTANLNAWLNNFYNAEA
                     KRKSTFPSSLPADAQPFELLVINICSLSWSDIEAAGLMSHPLWSHFDIEFKNFNSATS
                     HSGPAAIRLLRASCGQTSHTNLYQPANNDCYLFDNLSKLGFTQHLMMGHNGQFGGFLK
                     EVRENGGMQTELMDQTNLPVILLGFDGSPVYDDTAVLNRWLDVTEKDKNSRSATFYNT
                     LPLHDGNHYPGVSKTADYKARAQKFFDELDAFFTELEKSGRKVMVVVVPEHGGALKGD
                     RMQVSGLRDIPSPSITDVPVGVKFFGMKAPHQGAPIVIDQPSSFLAISDLVVRVLDGK
                     IFTEDNVDWKKLTSGLPQTAPVSENSNAVVIQYQDKPYVRLNGGDWVPYPQ"
     gene            complement(2078..2269)
                     /gene="bcsF"
                     /locus_tag="PN977_003370"
     CDS             complement(2078..2269)
                     /gene="bcsF"
                     /locus_tag="PN977_003370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsF"
                     /protein_id="HDI6043374.1"
                     /translation="MMTISDIIEIIVVCALIFFPLGYLARHSLRRIRDTLRLFFAKPR
                     YVKPAGTLRRTEKARATKK"
     gene            complement(2266..3837)
                     /gene="bcsE"
                     /locus_tag="PN977_003371"
     CDS             complement(2266..3837)
                     /gene="bcsE"
                     /locus_tag="PN977_003371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsE"
                     /protein_id="HDI6043375.1"
                     /translation="MRDIVDPVFSIGISSLWDELRHMPAGGVWWFNVDRHEDAISLAN
                     QTIASQAETAHVAVISMDSDPAKIFQLDDSQGPGKITLFSMLNHEKGLYYLARDLQCS
                     IDPHNYLFILVCANNAWQNIPAERLRSWLDKMNKWSRLNHCSLLVINSGNNNDKQFSL
                     LLEEYRSLFGLASLRFQGDQHLLDIAFWCNEKGVSARQQLSVQQQNGCWTLVQHQEAE
                     IQPRSDEKRILSNVSVLEGAPPLSEHWQLFNNNEVLFNEARTAQAATVVFSLQQNAQI
                     EPLARSIHTLRRQRGSAMKILVRENTASLRATDERLLLACGANMVIPWNAPLSRCLTM
                     IESVQGQKFSRYVPEDITTLLSMTQPLKLRGFQKWDVFCNAVNNMMNNPLLPAHGKGV
                     LVALRPVPGIRVEQALTLCRPNRTGDIMTIGGNRLVLFLSFCRINDLDTALNHIFPLP
                     TGDIFSNRMVWFEDDQISAELVQMRLLAPEQWGMPLPLTQSSKPVINAEHDGRHWRRI
                     PEPMRLLDDAVERSS"
     gene            4109..4297
                     /locus_tag="PN977_003372"
     CDS             4109..4297
                     /locus_tag="PN977_003372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhjR family protein"
                     /protein_id="HDI6043376.1"
                     /translation="MNNNEPDTLPDPAIGYIFQNDILALKQAFSLPDIDYADISQREQ
                     LAAALKRWPLLAEFAQQK"
     gene            4309..5061
                     /gene="bcsQ"
                     /locus_tag="PN977_003373"
     CDS             4309..5061
                     /gene="bcsQ"
                     /locus_tag="PN977_003373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsQ"
                     /protein_id="HDI6043377.1"
                     /translation="MAVLGLQGVRGGVGTTTITAALAWSLQMLGENVLVVDACPDNLL
                     RLSFNVDFTHRQGWARAMLDGQDWRDAGLRYTSQLDLLPFGQLSIEEQENPQHWQTRL
                     SDICSGLQQLKASGRYQWILIDLPRDASQITHQLLSLCDHSLAIVNVDANCHIRLHQQ
                     ALPDGAHILINDFRIGSQVQDDIYQLWLQSQRRLLPILIHRDEAMAQCLAAKQPVGEY
                     RSDALAAEEILTLANWCLLNYSGLKTPVGSAS"
     gene            5058..7676
                     /gene="bcsA"
                     /locus_tag="PN977_003374"
     CDS             5058..7676
                     /gene="bcsA"
                     /locus_tag="PN977_003374"
                     /EC_number="2.4.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417990.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-forming cellulose synthase catalytic
                     subunit"
                     /protein_id="HDI6043378.1"
                     /translation="MSILTRWLLIPPVNARLIGRYRDYRRHGASAFSATLGCFWLILA
                     WIFIPLEHPRWQRIRAEHKNLYPHINASRPRPLDPVRYLIQTCWLLIGTSRKETPKPR
                     RRTFSGLQNIRGRYHQWMNELPERVSHKTQHLDEKKELGHLSAGARRLILGIIVTFSL
                     ILALICVTQPFNPLAQFIFLMLLWGVALIVRRMPGRFSALMLIVLSLTVSCRYIWWRY
                     TSTLNWDDPVSLVCGLILLFAETYAWIVLVLGYFQVVWPLNRQPVPLPKDMSLWPSVD
                     IFVPTYNEDLNVVKNTIYASLGIDWPKDKLNIWILDDGGREEFRQFAQNVGVKYIART
                     THEHAKAGNINNALKYAKGEFVSIFDCDHVPTRSFLQMTMGWFLKEKQLAMMQTPHHF
                     FSPDPFERNLGRFRKTPNEGTLFYGLVQDGNDMWDATFFCGSCAVIRRKPLDEIGGIA
                     VETVTEDAHTSLRLHRRGYTSAYMRIPQAAGLATESLSAHIGQRIRWARGMVQIFRLD
                     NPLTGKGLKFAQRLCYVNAMFHFLSGIPRLIFLTAPLAFLLLHAYIIYAPALMIALFV
                     LPHMIHASLTNSKIQGKYRHSFWSEIYETALAWYIAPPTLVALINPHKGKFNVTAKGG
                     LVEEEYVDWVISRPYIFLVLLNLVGVAVGIWRYFYGPPTEMLTVVVSMVWVFYNLIVL
                     GGAVAVSVESKQVRRSHRVEMTMPAAIAREDGHLFSCTVQDFSDGGLGIKINGQAQIL
                     EGQKVNLLLKRGQQEYVFPTQVARVMGNEVGLKLMPLTTQQHIDFVQCTFARADTWAL
                     WQDSYPEDKPLESLLDILKLGFRGYRHLAEFAPSSVKGIFRVLTSLVSWVVSFIPRRP
                     ERSETAQPSDQALAQQ"
     gene            7687..10026
                     /gene="bcsB"
                     /locus_tag="PN977_003375"
     CDS             7687..10026
                     /gene="bcsB"
                     /locus_tag="PN977_003375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis cyclic di-GMP-binding
                     regulatory protein BcsB"
                     /protein_id="HDI6043379.1"
                     /translation="MKRKLFWICAVAMGMSAFPSFMTQATPATQPLINAEPAVAAQTE
                     QNPQVGQVMPGVQGADAPIVAQNGPSRDVKLTFAQIAPPPGSMVLRGINPNGSIEFGM
                     RSDEVVTKAMLNLEYTPSPSLLPVQSQLKVYLNDELMGVLPVTKEQLGKKTLAQMPIN
                     PLFITDFNRVRLEFVGHYQDVCENPASTTLWLDVGRSSGLDLTYQTLNVKNDLSHFPV
                     PFFDPRDNRTNTLPMVFAGAPDVGLQQASAIVASWFGSRSGWRGQNFPVLYNQLPDRN
                     AIVFATNDKRPDFLRDHPAVKAPVIEMINHPQNPYVKLLVVFGRDDKDLLQAAKGIAQ
                     GNILFRGESVVVNEVKPLLPRKPYDAPNWVRTDRPVTFGELKTYEEQLQSSGLEPAAI
                     NVSLNLPPDLYLMRSTGIDMDINYRYTMPPVKDSSRMDISLNNQFLQSFNLSSKQEAN
                     RLLLRIPVLQGLLDGKTDVSIPALKLGATNQLRFDFEYMNPMPGGSVDNCITFQPVQN
                     HVVIGDDSTIDFSKYYHFIPMPDLRAFANAGFPFSRMADLSQTITVMPKAPNEAQMET
                     LLNTVGFIGAQTGFPAINLTVTDDGSTIQGKDADIMIVGGIPDKLKDDKQIDLLVQAT
                     ESWVKTPMRQTPFPGIVPDESDRAAETQSTLTSSGAMAAVIGFQSPYNDQRSVIALLA
                     DSPRGYEMLNDAVNDSGKRATMFGSVAVIRESGINSLRVGDVYYVGHLPWFERLWYAL
                     ANHPILLAVLAAISVVLLAWVLWRLLRIISRRRLNPDNE"
     gene            10033..11139
                     /gene="bcsZ"
                     /locus_tag="PN977_003376"
     CDS             10033..11139
                     /gene="bcsZ"
                     /locus_tag="PN977_003376"
                     /EC_number="3.2.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase"
                     /protein_id="HDI6043380.1"
                     /translation="MNVLRSGLVTMLLLAAFSVQAACNWPAWEQFKKDYISQEGRVID
                     PSDARKITTSEGQSYGMFFALAANDRAAFDNILDWTQNNLAQGSLKERLPAWLWGKKE
                     NSKWEVLDSNSASDGDVWMAWSLLEAGRLWKEQRYTDIGSALLKRIAREEVVTVPGLG
                     SMLLPGKVGFAEDNSWRFNPSYLPPTLAQYFTRFGAPWTTLRETNQRLLLETAPKGFS
                     PDWVRYEKDKGWQLKAEKTLISSYDAIRVYMWVGMMPDSDPQKARMLNRFKPMATFTE
                     KNGYPPEKVDVATGKAQGKGPVGFSAAMLPFLQNRDAQAVQRQRVADNFPGSDAYYNY
                     VLTLFGQGWDQHRFRFSTKGELLPDWGQECANSH"
     gene            11121..14594
                     /gene="bcsC"
                     /locus_tag="PN977_003377"
     CDS             11121..14594
                     /gene="bcsC"
                     /locus_tag="PN977_003377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026226.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsC"
                     /protein_id="HDI6043381.1"
                     /translation="MRKFTLNIFTLSLGLAVMPMVEAAPTAQQQLLEQVRLGEATHRE
                     DLVQQSLYRLELIDPNNPDVVAARFRSLLRQGDIDGAQKQLDRLSQLAPSSNAYKSSR
                     TTMLLSTPDGRQALQQARLQATTGHAEEAVASYNKLFNGAPPEGDIAVEYWSTVAKIP
                     ARRGEAINQLKRINADTPGNTGLQNNLALLLFSSDRRDEGFAVLEQMAKSNAGREGAS
                     KIWYGQIKDMPVSDASVSALKKYLSIFSDGDSVAAAQSQLAEQQKQLADPAFRARAQG
                     LAAVDSGMAGKAIPELQQAVRANPKDSEALGALGQAYSQKGDRANAVANLEKALALDP
                     HSSNNDKWNSLLKVNRYWLAIQQGDAALKANNPDRAERLFQQARNVDNTDSYAVLGLG
                     DVAMARKDYPAAERYYQQTLRMDSGNTNAVRGLANIYRQQSPEKAEAFIASLSASQRR
                     SIDDIERSLQNDRLAQQAEALENQGKWGQAAALQRQRLALDPGSVWITYRLSQDLWQA
                     GQRSQADTLMRNLAQQKPNDPEQVYAYGLYLSGHDQDRAALAHINSLPRAQWNSNIQE
                     LVNRLQSDQVLETANRLRENGKEAEAEAMLRQQPPSTRIDLTLADWAQQRRDYTAARA
                     AYQNVLTREPTNADAILGLTEVDIAAGDKAAARSQLAKLPATDNASLNTQRRVALAQA
                     QLGDTAAAQQTFNKLIPQAKSQPPSMESAMVLRDGAKFEAQAGDPTQALETYKDAMVA
                     SGVTTTRPQDNDTFTQLTRNDEKDDWLKRGVRSDAADLYRQQDLNVTLEHDYWGSSGT
                     GGYSDLKAHTTMLQVDAPYSDGRMFFRSDFVNMNVGSFSTNADGKWDDNWGTCTLQDC
                     SGNRSQSDSGASVAVGWRNDVWSWDIGTTPMGFNVVDVVGGISYSDDIGPLGYTVNAH
                     RRPISSSLLAFGGQKDSPSNTGKKWGGVRADGVGLSLSYDKGEANGVWASLSGDQLTG
                     KNVEDNWRVRWMTGYYYKVINQNNRRVTIGLNNMIWHYDKDLSGYSLGQGGYYSPQEY
                     LSFAIPVMWRERTENWSWELGASGSWSHSRTKTMPRYPLMNLIPTDWQEEAARQSNDG
                     GSSQGFGYTARALLERRVTSNWFVGTAIDIQQAKDYAPSHFLLYVRYSAAGWQGDMDL
                     PPQPLIPYADW"
     gene            14676..16664
                     /gene="hmsP"
                     /locus_tag="PN977_003378"
     CDS             14676..16664
                     /gene="hmsP"
                     /locus_tag="PN977_003378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator HmsP"
                     /protein_id="HDI6043382.1"
                     /translation="MRVSRSLTIKQMAMVAAVVLVFVFIFCTVLLFHLVQQNRYNTAT
                     QLESIARSVREPLSSAILKGDIPEAEAILASIKPAGVVSRADVVLPNQFQALRKSFIP
                     ERPVPVMVTRLFELPVQISLGVYSLERPANPQPIAYLVLQADSFRMYKFVMSTLSTLV
                     TIYLLLSLILTVAISWCINRLILHPLRNIARELNAIPAQELVGHQLALPRLHQDDEIG
                     MLVRSYNLNQQLLQRHYEEQNENAMRFPVSDLPNKALLMEMLEQVVARKQTTALMIIT
                     CETLRDTAGVLKEAQREILLLTLVEKLKSVLSPRMILAQISGYDFAVIANGVQEPWHA
                     ITLGQQVLTIMSERLPIERIQLRPHCSIGVAMFYGDLTAEQLYSRAISAAFTARHKGK
                     NQIQFFDPQQMEAAQQRLTEESDILNALENHQFAIWLQPQVEMTSGKLVSAEVLLRIQ
                     QPDGSWDLPDGLIDRIECCGLMVTVGHWVLEESCRLLAAWQERGIMLPLSVNLSALQL
                     MHPNMVADMLELLTRYRIQPGTLILEVTESRRIDDPHAAVAVLRPLRNAGVRVALDDF
                     GMGYAGLRQLQHMKSLPIDVLKIDKMFVEGLPEDSSMIAAIIMLAQSLNLQMIAEGVE
                     TEAQRDWLAKAGVGIAQGFLFARPLPIEIFEESYLEEK"
     gene            16847..18133
                     /gene="dctA"
                     /locus_tag="PN977_003379"
     CDS             16847..18133
                     /gene="dctA"
                     /locus_tag="PN977_003379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate transporter DctC"
                     /protein_id="HDI6043383.1"
                     /translation="MKTSLFKSLYFQVLTAIAIGILLGHFYPEIGEQMKPLGDGFVKL
                     IKMIIAPVIFCTVVTGIAGMESMKAVGRTGAVALLYFEIVSTIALIIGLIIVNVVQPG
                     AGMNVDPATLDAKAVAVYADQAKDQGIVAFIMDVIPASVIGAFASGNILQVLLFAVLF
                     GFALHRLGSKGQLIFNVIESFSQVIFGIINMIMRLAPIGAFGAMAFTIGKYGVGTLVQ
                     LGQLIICFYITCILFVVLVLGSIAKATGFSIFKFIRYIREELLIVLGTSSSESALPRM
                     LDKMEKLGCRKSVVGLVIPTGYSFNLDGTSIYLTMAAVFIAQATNSQMDIVHQITLLI
                     VLLLSSKGAAGVTGSGFIVLAATLSAVGHLPVAGLALILGIDRFMSEARALTNLVGNG
                     VATIVVAKWVKELDHKKLDDVLNNRAPDGKTHELSS"
     gene            18353..>18576
                     /locus_tag="PN977_003380"
     CDS             18353..>18576
                     /locus_tag="PN977_003380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HDI6043384.1"
                     /translation="MQGTKIRLLAGGLLMMATAGYVQADALQPDPAWQQGTLSNGLQW
                     QVLTTPQRPSDRVEIRLLVNTGSLAESTQQ"
BASE COUNT         4320 a         4765 c         5163 g         4328 t
ORIGIN      
        1 aacagtcgta gaatcagtgg caagccggaa gggttccggt gaggcgcaat gttgcggggg
       61 gcttaatccc tggtggcatt ggttgctgga gagagaaaac ccccgcacgt tgcaggtatg
      121 cacctgacaa catcacgggg gctaatcttg aaccttaaca actcaagaat agccgcgaaa
      181 cgttgtcatt acaacagagg cggctatatg acgctcgcag aactgggcat ggccttctgg
      241 catgatttag cggctccggt cattgctggc attcttgcca gtctgatcgt gaactcgctg
      301 aataagcgga agtaacgtgt catgcgggcg tcaggctgcc gtaatggcaa gttgcgcccg
      361 gaccaggccg caggggtgaa actctgcggc ctttttcgtt cttattgcgg gtaaggcacc
      421 cagtcgccgc cgttcaggcg aacgtacggt ttatcctggt attgaataac tactgcattt
      481 gagttctcgg agaccggtgc tgtttgtggc aacccactgg tgagtttttt ccagtcaaca
      541 ttgtcttcgg tgaaaatctt gccatcaaga acccgaacca ccagatcgga gatagccagg
      601 aagctgctcg gttggtcgat gacaatcggt gccccctgat gtggtgcctt catgccgaag
      661 aatttcaccc caacggggac gtctgtgata gacgggctag ggatatcacg taggccagat
      721 acctgcattc tgtcgccctt cagcgcgccg ccgtgttctg gcaccacgac caccatcact
      781 ttacgacccg atttctccag ttcagtaaag aaggcgtcca gttcatcaaa gaatttctgc
      841 gcccgcgctt tgtaatccgc tgttttgctg acgcccggat aatggttgcc gtcatgcagt
      901 ggaagcgtgt tgtagaacgt ggcactacgg ctgtttttat ctttttcggt aacgtccagc
      961 cagcggttaa gcacggcggt atcgtcataa accggcgaac catcaaagcc cagcaaaata
     1021 accggcagat ttgtttgatc catcaattcg gtctgcatgc caccattttc gcgaacttct
     1081 ttcaaaaaac cgccgaactg gccgttatgt cccatcatca ggtgctgggt aaagcccagt
     1141 ttcgacaggt tatcaaacag gtagcagtca ttatttgccg gttgatacag attagtgtgc
     1201 gaagtctgcc cgcagctggc gcgcagtaaa cggatcgccg ccgggccact gtgggaggtg
     1261 gcggagttaa agttcttgaa ctcaatatcg aaatgcgacc acagcggatg cgacatcaac
     1321 ccggcggctt caatatccga ccaggaaagc gaacagatat taatcaccag tagttcaaat
     1381 ggctgagcat cagcgggcag cgaagacggg aaggtcgatt tacgtttcgc ctccgcgtta
     1441 tagaaattat tcagccaggc gttaaggttc gccgttgttg gcggtgcagt ttgtgcgggc
     1501 atatcaccca ctaccggtgc gccacccgtc gccgcaacgg ttgccgctgc gttaccaccc
     1561 gtcgttgtta cagtggtcgt cggttgaccg gcaggccaca aggagaaact tggtcccgcc
     1621 agggtaagta cgttcagcca tagcagaatg gcaaccacaa aaacggtaat gcgaatccat
     1681 tgtgacagga ataaccaggc cactaataaa acaaaaatgg ccccaatcat ctgccagtta
     1741 ataaagcgtg tgacaaggtc gattaaataa tcggtactga accccgccac ctgcgaaccc
     1801 tggctcatta tgctttccgg gccaggcaac caggtgtcat gccagaacaa agcaaagccg
     1861 atcggcaggg caatccagtg gcgtaagcga tgcaggctgt agcgcggaat gggcatcagc
     1921 agaaacgcgg caaacaccag attgaggagc ggatggaagt taagatatcc tgcccacaac
     1981 aggccgaact taaccagaaa atagaagttc cagccggaaa ggccgcgcca gtattgccag
     2041 agggaagaag gcatggcggt attttgcgta aattgagtca ttttttggtt gccctggctt
     2101 tttccgtgcg gcgtaacgtc ccggccggtt taacataacg aggtttagca aagaacaaac
     2161 gtaaggtgtc gcgaatgcgt cgcaaagagt gccgcgccag atagcccagc gggaaaaata
     2221 tcagtgcgca aacgacaata atttcaatga tatcgctgat ggtcatcatg atgagcgctc
     2281 cacagcatca tctaacagtc gcattggttc tggtattcgt cgccagtggc gaccatcgtg
     2341 ctcggcattg atgaccggtt tagaactttg cgttaaaggc agcggcatgc cccattgttc
     2401 tggggcaagc aggcgcatct gcaccagctc ggcactgatt tgatcatctt caaaccagac
     2461 catacggttt gagaaaatgt cgccagtcgg caatgggaaa atatgattca acgcggtatc
     2521 cagatcgtta atccgacaga atgagagaaa cagcaccagc cgattaccgc caatggtcat
     2581 gatatcgcca gtgcgattag ggcgacatag cgtcagggct tgctcaacgc ggatacccgg
     2641 taccggacgt agggcaacca gaacgccttt accgtgggca ggtaatagag ggttattcat
     2701 catgttgttg acggcattac agaacacatc ccacttctgg aaaccacgca gtttgagcgg
     2761 ctgggtcatt gacagcaagg tagtgatatc ttccggcaca tagcgactaa acttctgccc
     2821 ttgcacgctt tcgatcatcg tcagacagcg ggagagtggg gcattccatg ggataaccat
     2881 atttgcaccg caggccaata acaaacgttc atcggtggcg cgcaggctag cggtattttc
     2941 ccgtacgagg attttcatcg cactaccgcg ctggcgacgc agagtatgaa tgctgcgggc
     3001 cagtggctcg atttgcgcat tttgttgtaa agaaaagacc accgtcgccg cctgagcggt
     3061 acgggcttca ttaaacagga cttcgttatt gttgaacagt tgccagtgtt ccgatagcgg
     3121 cggcgcacct tcaagtacag aaacattact cagaatgcgt ttttcgtcgc tgcgtggttg
     3181 gatttccgcc tcttggtgtt gaactaatgt ccagcaacca ttttgttgct gaacgctaag
     3241 ctgctgacgg gcgctgaccc ctttttcgtt gcaccagaaa gcaatatcca gcaaatgttg
     3301 gtcgccctga aaacgcaaac tggcaagacc aaaaagtgaa cggtattcct caagtaacaa
     3361 tgaaaattgt ttatcgttat tatttccgga attaattacc aaaagcgaac aatggtttag
     3421 ccggctccat ttattcattt tatccaacca tgagcgaagc cgctcggcag gaatgttttg
     3481 ccatgcgtta tttgcgcaaa caagaataaa aaggtaatta tggggatcaa tagaacactg
     3541 caaatcacgg gccaaatagt atagaccttt ttcatgattt agcattgaaa ataatgttat
     3601 ttttcccggc ccttgagaat catctaattg aaagattttc gctggatcgc tgtccatgct
     3661 aatgaccgcg acgtgtgcgg tttcagcctg ggatgcaatt gtttgattcg ccagactgat
     3721 agcatcttca tggcgatcga cgttaaacca ccagacgccg cctgctggca tatgtcgcag
     3781 ctcatcccat aatgatgaga taccgataga gaatacaggg tccacaatgt ccctcatgtc
     3841 aacattttgt ctgattatca gtttactagc gaaagctaag aaataaacct accattgaaa
     3901 ttaaaactta tcagatttag catgtgaatc aatttagcag gtcacaagac atgtgatttt
     3961 agtcttttcg tttttaactt tttctgttgc gtaacggaaa gtcaaaaagt gagcacattc
     4021 ccgtctcgcc gtaaacaata aaccggcgaa atgctcacgg ttagaattgt ctcatgaacg
     4081 gtacggttat ttcataggga tcaagcaaat gaataacaat gaaccagata ctctgcctga
     4141 tcccgcgata ggctatatct tccagaatga tattttggcg ttaaagcagg cattttcact
     4201 gcctgatatt gattatgccg atatttccca acgcgaacag ttggccgcgg cattaaaacg
     4261 ctggccgttg ctggcagagt ttgcgcaaca aaagtagggg attggtgaat ggccgtactg
     4321 ggattgcagg gggtgcgggg aggcgtgggg acaacaacca tcaccgccgc attagcctgg
     4381 tcattacaaa tgttgggaga aaatgtcctg gtggtcgatg cctgcccgga caacttgttg
     4441 cgcctgtcat ttaacgttga ttttacccac cgtcagggct gggccagagc tatgctggat
     4501 ggccaggact ggcgtgacgc cgggttgcgc tatacctcgc agctcgattt gctgcctttt
     4561 ggtcagttat ccattgaaga acaagaaaat ccacagcact ggcaaacccg gctgagcgat
     4621 atttgctccg gcttacagca actaaaagcc agcgggcgtt accagtggat tttaattgac
     4681 ttaccgcgtg atgcctcaca gataacccac cagctgctga gtttgtgcga tcactcgctg
     4741 gcaatcgtca atgtggatgc caactgccat atccgactgc atcagcaagc gctgccggat
     4801 ggcgcgcata ttttgattaa tgacttccgt atcggcagtc aggttcagga cgatatttac
     4861 cagctttggt tgcaaagcca gcgccgatta ctgccaatac tcattcatcg tgatgaagcg
     4921 atggctcaat gcctggcggc taagcaacca gtcggtgaat accgcagtga tgcgctggcg
     4981 gctgaagaga tactgacgct ggctaactgg tgcctgttga actattccgg gctgaaaacg
     5041 ccagtcggga gtgcatcatg agtatcctga cccggtggtt gcttatcccg ccggtcaacg
     5101 cgcggcttat cgggcgttat cgcgattatc gtcgtcacgg tgcgtcggct ttcagcgcga
     5161 cgctcggctg tttttggttg atcctggcct ggatttttat tccgctggag cacccgcgct
     5221 ggcagcgtat tcgcgcagaa cataaaaacc tgtatccgca tatcaacgcc tcgcgtccgc
     5281 gtccgctgga cccggtccgt tatctcattc aaacatgctg gttactgatc ggtacatcgc
     5341 gcaaagaaac gccgaaaccg cgcaggcgga cattttcagg tctgcagaat attcgtggac
     5401 gttaccatca atggatgaac gagctgcctg agcgcgttag ccataaaaca cagcatctcg
     5461 atgagaaaaa agaactcggc catttgagcg ccggggcgcg gcggttgatc ctcggtatca
     5521 tcgtcacctt ctcgctgatt ctggcgttaa tctgcgttac ccagccgttt aacccgctgg
     5581 cgcagtttat cttcctgatg ctgctgtggg gtgtagcgct gatcgtacgg cggatgccgg
     5641 ggcgcttctc ggcgctaatg ttgattgtgc tgtcgctgac cgtttcttgc cgttatatct
     5701 ggtggcgcta cacctctacg ctgaactggg acgatccggt cagcctggtg tgcgggctta
     5761 ttctgctgtt cgccgaaacg tacgcgtgga ttgtgctggt gctcggttac ttccaggtgg
     5821 tatggccgct gaatcgtcag ccggtgccat tgccgaaaga tatgtcgctg tggccgtcgg
     5881 tggatatctt tgtcccgact tacaacgaag atctcaacgt ggtgaaaaat acgatttacg
     5941 cctcgctggg tatcgactgg ccgaaagaca agctgaacat ctggatcctc gatgacggcg
     6001 gcagggaaga gtttcgccag tttgcgcaaa acgtgggggt gaaatatatc gcccgtacca
     6061 ctcatgaaca tgcgaaagcg ggcaacatca acaatgcgct gaaatatgcc aaaggcgagt
     6121 tcgtgtcgat tttcgactgc gaccacgtac caacgcgatc gttcctgcaa atgaccatgg
     6181 gctggttcct gaaagaaaaa caactggcga tgatgcagac gccgcaccac ttcttctcac
     6241 cggacccgtt tgaacgcaac ctgggacgtt tccgtaaaac accgaacgaa ggcacgctgt
     6301 tctatggtct ggtgcaggac ggcaacgata tgtgggacgc caccttcttc tgcggttcat
     6361 gtgcggtgat tcgccgtaaa ccgctggatg aaattggcgg cattgctgtc gaaacggtaa
     6421 ctgaagatgc gcatacttct ctgcgtttgc accgtcgtgg ctatacctcc gcgtatatgc
     6481 gtattccgca ggcggcgggg ctggcgaccg aaagtctgtc ggcgcatatc ggtcagcgta
     6541 ttcgctgggc gcgcgggatg gtacaaatct tccgtctcga taacccgctc accggtaaag
     6601 ggctgaagtt tgctcagcgg ctatgttacg tcaacgccat gttccacttc ttgtcgggca
     6661 ttccacggct gatcttcctg actgcgccgc tggcgttcct gctgcttcat gcctacatca
     6721 tctatgcgcc agcgttgatg attgccctgt tcgtgctgcc gcatatgatc catgccagcc
     6781 tgaccaactc caagatccag ggcaaatatc gccactcttt ctggagtgaa atctacgaaa
     6841 cggcgctggc gtggtatatc gcaccaccga cgctggtggc gctgattaac ccgcacaaag
     6901 gcaaatttaa cgtcaccgcc aaaggtggac tggtggaaga agagtacgtc gactgggtga
     6961 tctcgcggcc ctacatcttc cttgttctgc tcaacctggt gggcgttgcg gtaggcatct
     7021 ggcgctactt ctatggcccg ccaaccgaga tgctcaccgt ggtcgtcagt atggtgtggg
     7081 tgttctacaa cctgattgtt cttggcggcg cagttgcggt atcggtagaa agcaaacagg
     7141 tacgccgatc gcaccgcgtg gagatgacga tgcccgcggc aattgcccgc gaagatggtc
     7201 acctcttttc gtgtaccgtt caggatttct ccgacggtgg tttggggatc aagatcaacg
     7261 gtcaggcgca gattctggaa gggcagaaag tgaatctgtt gcttaaacgt ggtcagcagg
     7321 aatacgtctt cccgacccag gtggcgcgcg tgatgggtaa tgaagttggg ctgaaattaa
     7381 tgccgctcac cacccagcaa catatcgatt ttgtgcagtg tacgtttgcc cgtgcggata
     7441 catgggcgct ctggcaggac agctatccgg aagataagcc gctggaaagt ctgctggata
     7501 ttctgaagct cggcttccgt ggctaccgcc atctggcgga gtttgcgcct tcttcggtga
     7561 agggcatatt ccgtgtgctg acttctctgg tttcctgggt tgtatcgttt attccgcgcc
     7621 gcccggagcg gagcgaaacg gcacaaccat cggatcaggc tttggctcaa caatgatgat
     7681 aacgcgatga aaagaaaact attctggatt tgtgcagtgg ctatggggat gagtgcgttc
     7741 ccctctttca tgacgcaggc gacgccagca acgcaaccac tgatcaatgc tgagccagct
     7801 gtagccgccc agacggaaca aaatccgcag gtggggcaag tgatgccggg cgtgcagggc
     7861 gctgatgccc ccatcgtggc gcagaacggt ccttcgcgtg atgtgaagct gacctttgcg
     7921 caaattgcgc cgccgccggg cagcatggtg ctacgtggca ttaacccgaa cggcagcatt
     7981 gagtttggta tgcgcagtga tgaagtggtg acgaaggcga tgctcaacct cgaatacacg
     8041 ccatcgccat cgttactgcc tgtccagtcg cagttaaagg tttatctcaa tgatgaactg
     8101 atgggcgtgc tgccagtgac caaagaacag ttgggtaaaa aaacgctggc gcaaatgccc
     8161 attaacccac tgtttattac cgacttcaac cgtgtgcggc tggagtttgt cggccattat
     8221 caggacgtgt gcgaaaaccc ggccagcacc acgctttggc tggatgttgg gcggagcagt
     8281 ggactggatt tgacctatca gaccctgaat gtgaagaatg acctgtcaca cttcccggtg
     8341 ccattctttg acccgcgtga taaccgcacc aacaccttgc cgatggtctt tgcgggtgcg
     8401 ccggatgttg ggctgcaaca agcatctgcc attgtcgcct cgtggtttgg ttcgcgttct
     8461 ggctggcgtg ggcagaactt cccggtgctc tataaccaac tgccggatcg caatgccatt
     8521 gtttttgcca ctaatgacaa acggccggac ttcctgcgcg atcatccggc ggtaaaagcc
     8581 ccggtgattg aaatgatcaa ccatccgcag aatccttacg tcaaactgct ggtggtcttt
     8641 ggtcgtgacg acaaagacct gttgcaggca gcgaaaggta tcgctcaggg taacattctg
     8701 ttccgtggtg aaagcgtggt agtgaatgaa gtgaaaccgc tgctaccgcg taagccgtac
     8761 gatgcgccga actgggtgcg taccgatcgt ccggtcacct ttggtgaact gaaaacctat
     8821 gaagaacagt tacaatccag cggtcttgag ccagcagcga ttaacgtttc gctaaatctg
     8881 ccgccggatc tctacctgat gcgcagtacc ggcattgata tggatatcaa ctatcgctac
     8941 accatgccgc cggtgaaaga cagttcgcgg atggatatca gcctgaataa ccagttcctg
     9001 caatccttca acctgagcag taaacaggag gcgaaccgcc tgctgctgcg gattccggta
     9061 ttacaaggtt tgctggatgg caaaacggat gtctctattc cggcgctgaa actgggcgcg
     9121 accaaccagt tgcggttcga ctttgagtat atgaacccga tgccgggcgg ttcggtggat
     9181 aactgtatta ccttccagcc ggtgcagaat catgtggtga ttggtgacga ctccaccatc
     9241 gacttctcga agtattacca cttcatcccg atgccggatc tacgcgcctt tgctaacgcg
     9301 ggcttcccgt tcagccggat ggcggatctg tcacaaacca tcaccgtgat gccgaaagcg
     9361 cctaacgaag cacagatgga aaccttgctg aataccgttg gctttatcgg tgcgcagacg
     9421 ggcttcccgg cgattaacct gacggtgacc gatgatggca gcaccattca gggcaaagat
     9481 gccgacatca tgatcgtcgg tggtatcccg gacaaactga aagacgacaa gcagatcgac
     9541 ctgttggtgc aggcgaccga aagctgggtg aaaacgccga tgcgccagac cccgttcccc
     9601 ggcattgtac cggacgagag cgatcgcgcg gcagaaactc agtcaacgct gacctcttcc
     9661 ggtgcgatgg cagcggtgat tggcttccag tcgccgtata acgaccagcg tagcgtgatt
     9721 gcgctgctgg cagatagccc acgcggttat gaaatgctta acgatgcggt gaacgatagc
     9781 ggcaaacgcg ccaccatgtt cggttcggtc gcggtgatcc gcgagtccgg tatcaatagc
     9841 ctgcgtgttg gcgacgtcta ttacgttggc catctgccgt ggttcgaacg cttatggtat
     9901 gccctggcaa accatccgat tctgctggcg gtgctggcgg caatcagcgt cgtgctgctg
     9961 gcatgggtac tgtggcgttt gctgcgtatt atcagccgtc gtcgtcttaa cccggataac
    10021 gagtaattga agatgaatgt gttgcgtagt ggactcgtga cgatgctgct gctggctgcc
    10081 tttagtgttc aggcagcctg caactggcct gcctgggagc aattcaaaaa ggattacatc
    10141 agtcaggaag ggcgcgtcat tgacccaagt gacgcgcgta aaatcaccac ttccgaaggg
    10201 caaagttacg gcatgttctt tgccctggcg gctaacgacc gtgcagcttt cgataatatt
    10261 ctcgactgga cgcagaacaa tctcgctcag ggttctttaa aagaacgttt gcccgcatgg
    10321 ctgtggggca agaaagagaa cagtaagtgg gaagtgctgg acagcaattc ggcctccgat
    10381 ggtgatgtct ggatggcctg gtcgttgctg gaggcggggc gtttgtggaa agagcagcgt
    10441 tataccgaca tcggcagcgc gttgttaaaa cgtattgcgc gggaggaagt ggtgacggtg
    10501 cctgggctgg gttccatgtt gttgccgggc aaagtgggat ttgcagagga taacagctgg
    10561 cgttttaacc ccagctacct gccgccgacg ctggcgcagt atttcacccg ctttggcgcg
    10621 ccgtggacta cgctgcgcga aaccaatcaa cgtttattgc tggaaaccgc cccgaaaggc
    10681 ttttcgccag actgggtgcg ctatgagaaa gacaaaggct ggcagctaaa agccgaaaaa
    10741 acattgatca gcagctacga cgcgatccgc gtttacatgt gggtaggcat gatgcctgac
    10801 agcgatccgc aaaaagcgcg gatgctcaac cggtttaaac cgatggcgac attcaccgag
    10861 aaaaacggtt atccgccgga aaaagtggat gtggctacgg ggaaagcaca gggtaaagga
    10921 ccggtcggtt tttctgccgc catgctgccc tttttacaaa accgcgatgc gcaggccgtt
    10981 cagcgccagc gcgtggccga taactttccc ggcagcgatg cctattacaa ctatgtgctg
    11041 accctgtttg gacaaggctg ggatcaacac cgtttccgct tctcgacaaa aggtgagtta
    11101 ttacctgact ggggccagga atgcgcaaat tcacactaaa catattcacg ctttccctcg
    11161 gtctggccgt catgccgatg gtcgaggcag caccaaccgc tcagcaacag ttgctggagc
    11221 aagttcggtt aggcgaagcg acccatcgtg aagacctggt gcaacagtcg ttgtatcgtc
    11281 tggaacttat tgatccgaat aacccggacg tcgttgccgc ccgtttccgt tctctgttac
    11341 gtcagggcga tattgatggc gcgcaaaaac agctcgatcg gctgtcgcag ttagcgccga
    11401 gttcaaatgc gtataaatcg tcgcggacca caatgttgct ttccacgccg gatggtcgtc
    11461 aggcactgca acaggcaaga ttgcaggcga ctactggtca tgcagaagaa gctgtggcga
    11521 gttacaacaa actgttcaac ggtgcgccgc cggaaggtga cattgccgtc gagtactgga
    11581 gtacggtggc gaaaattccg gctcgccgtg gcgaagcgat taatcagcta aaacgcatta
    11641 atgcggatac gccgggcaat acgggcctgc aaaacaatct ggcgctattg ctgtttagta
    11701 gcgatcgccg tgacgaaggt tttgccgtcc tggaacagat ggcgaaatcg aacgccgggc
    11761 gcgaaggggc ctctaaaatc tggtacgggc agattaaaga catgcccgtc agcgatgcca
    11821 gtgtgtcggc actgaaaaaa tatctctcga tctttagcga tggcgatagc gtggcggctg
    11881 cacaatcgca actggcagaa cagcaaaaac agctggccga ccctgctttc cgcgctcgtg
    11941 cgcaaggttt agcggcggtg gactctggta tggcgggtaa agccattccc gaactacaac
    12001 aggcggtgcg ggcaaacccg aaagacagtg aggctctggg ggcgctgggc caggcgtatt
    12061 ctcagaaagg cgatcgcgcc aatgcggtgg cgaatctgga aaaagccctc gcactggacc
    12121 cgcacagcag caacaacgac aaatggaaca gtctgctgaa agtaaaccgc tactggctgg
    12181 caatccagca gggcgatgct gcgctgaaag ccaataatcc tgaccgggca gaacgcctgt
    12241 tccagcaggc gcgtaatgtc gataacaccg acagctatgc agtgcttggg ctgggcgatg
    12301 tggcgatggc gcgcaaagat taccccgccg ctgaacgcta ttatcagcag accctgcgta
    12361 tggacagcgg caacactaac gccgtgcgcg ggctggcaaa tatttaccgc cagcaatcgc
    12421 cagaaaaagc tgaagcgttt atcgcctcgc tctctgccag tcagcggcgt agcattgatg
    12481 atatcgaacg cagcctacaa aacgaccgtc tggcacagca ggcagaggca ctggaaaacc
    12541 agggcaaatg ggggcaggcg gcagcacttc agcggcaacg actggcgctg gatcccggca
    12601 gcgtatggat tacttaccga ctttcgcagg atctctggca ggccggacaa cgcagccagg
    12661 ctgatacatt aatgcgcaat ctggcacagc agaagccgaa cgacccggag caggtttacg
    12721 cttacgggct gtatctctct ggtcatgacc aggacagagc ggcgctggcg catatcaaca
    12781 gcctgccgcg cgcgcagtgg aacagcaata ttcaggaact ggttaatcgc ctgcaaagcg
    12841 atcaggtgct ggaaaccgct aatcgcctgc gagaaaacgg caaagaagct gaagcggaag
    12901 cgatgctgcg ccagcaacca ccttccacgc gcatagacct cacgctggct gactgggcgc
    12961 aacaacgacg tgattacacc gccgctcgcg ctgcatatca gaatgtcctg acgcgggagc
    13021 caactaacgc cgatgccatt cttggtctga cggaagtgga tattgctgcc ggtgacaaag
    13081 cggcggcacg tagccagctg gcgaaactgc ccgccactga taacgcctcg ctgaacactc
    13141 agcggcgcgt ggcgctggca caggcgcagc ttggcgatac cgcagcggcg cagcagacgt
    13201 ttaataagtt gatcccgcag gcaaaatctc agccaccgtc gatggaaagc gcgatggtgc
    13261 tgcgcgacgg tgcgaagttt gaagcgcagg cgggcgatcc aacgcaggcg ctggaaacct
    13321 acaaagacgc gatggtcgca tccggtgtga ccacgacgcg tccgcaggat aacgacacct
    13381 ttacccagct gacccgtaac gacgagaaag atgactggct gaaacgcggc gtgcgcagcg
    13441 atgcagcgga cctctatcgc cagcaggatc ttaacgtcac cctcgagcac gattactggg
    13501 gttcgagcgg caccggtggt tactccgatc tgaaagcgca caccactatg ttgcaggtgg
    13561 atgcgccgta ttctgacggg cggatgttct ttcgcagtga tttcgtcaat atgaacgtcg
    13621 gcagtttctc cactaatgcc gatggtaaat gggatgacaa ctggggcacc tgtacattac
    13681 aggattgtag cggcaaccgc agccagtcgg attccggtgc cagcgtggcg gtcggctggc
    13741 gaaatgacgt ctggagctgg gatatcggta ccacgccgat gggcttcaac gtggtggatg
    13801 tggttggcgg catcagttac agcgatgata tcgggccgct gggttacacc gttaacgccc
    13861 atcgtcggcc catctccagt tctttgctgg cctttggtgg gcaaaaagac tctccgagca
    13921 ataccgggaa aaaatggggc ggcgtgcgtg ccgacggcgt ggggctaagt ctgagttacg
    13981 ataaaggtga agcaaacggc gtctgggcat cgcttagcgg tgatcagtta actggtaaaa
    14041 atgtcgaaga taactggcgc gtgcgctgga tgacgggcta ttactataag gtcatcaacc
    14101 agaataatcg ccgtgtgacc attggtctta acaatatgat ctggcattac gacaaagacc
    14161 tgagtggcta ctcacttggt cagggcggtt actacagccc gcaggaatac ctgtcgtttg
    14221 ccataccggt gatgtggcgg gagcggacgg aaaactggtc gtgggagctg ggggcgtctg
    14281 gctcgtggtc gcattcacgc accaaaacca tgccgcgtta tccgctgatg aatctgatcc
    14341 cgaccgactg gcaggaagaa gctgcgcggc aatccaacga tggcggcagc agtcagggct
    14401 ttggctacac ggcgcgggca ttacttgaac gacgtgttac ctccaactgg tttgtcggca
    14461 cggcaattga tatccagcag gcaaaagatt acgcacccag ccatttcctg ctctacgtac
    14521 gttattccgc cgccggatgg cagggtgaca tggatttacc gccgcagccg ctgatacctt
    14581 acgccgactg gtaagttttc agatagcgcc tctcttaatg ccgctgcgat cgggtatact
    14641 cgggcggcaa tctgggattt ccggggggag acaatttgcg cgtaagtcgc tcgttaacaa
    14701 tcaagcagat ggcaatggtg gcagccgttg tcctggtgtt cgtttttatt ttttgcaccg
    14761 ttttgctgtt ccatctggtc cagcagaatc gctataacac ggctacgcaa ctggaaagca
    14821 ttgctcgctc tgtccgcgaa cccttatctt ctgccatttt gaaaggcgat attcccgaag
    14881 cggaagctat tcttgccagc attaaaccgg caggcgtggt cagccgtgcc gatgtggtgc
    14941 tgcctaacca gttccaggcg ctgcgtaaaa gttttattcc agagcgtccg gtgccggtaa
    15001 tggttactcg cctgtttgag ctaccggtgc aaatctcgct gggcgtttac tcgctggaac
    15061 gtccggcaaa cccgcagcca attgcctatc tggtgctaca ggcggattcc ttccgcatgt
    15121 ataagttcgt gatgagcacc ctctcaacgt tagtgaccat ttacttactt ttgtcgttaa
    15181 tattgacggt ggcgattagc tggtgcatta accgcctgat tttgcatcca ttacgcaata
    15241 ttgctcgcga acttaacgcc atcccagccc aggagcttgt tggtcaccaa ctggcattac
    15301 cgcgtctgca tcaggacgat gaaatcggta tgttggtgcg cagttacaac ctcaaccagc
    15361 aattgctgca gcgccattat gaagaacaga acgaaaatgc gatgcgcttc ccggtgtcgg
    15421 atttgccgaa caaagccttg ctgatggaga tgctggagca ggtggtggcg cgtaaacaaa
    15481 ccaccgcgct gatgatcatc acctgtgaaa ccctgcgtga tactgcgggt gtgctgaaag
    15541 aggcgcaacg agaaattctg ctgctgacgc tggtggaaaa actcaaatcg gtactgtcgc
    15601 cacgtatgat cctcgcgcag attagcggtt atgactttgc tgtcattgcc aacggtgtac
    15661 aggaaccgtg gcacgcaatc actttgggtc agcaagtgct cactatcatg agcgagcgcc
    15721 tgccgattga acgtattcaa ctccgtccgc actgtagcat tggcgtggcg atgttctacg
    15781 gcgatctcac cgccgaacag ctttatagcc gcgctatttc tgcggcattt accgctcgcc
    15841 ataaaggcaa gaatcagatt cagttctttg atccgcagca gatggaagca gcccaacagc
    15901 ggttgacgga agagagcgat atccttaatg cactggaaaa tcatcagttt gcgatttggt
    15961 tacagccaca ggtcgagatg accagcggta aactggtcag tgcggaagtg ttactgcgta
    16021 tccagcaacc ggatggcagt tgggatctgc cggatggctt aatcgatcgc attgaatgct
    16081 gtgggctgat ggttaccgtt ggtcactggg tgctggaaga gtcctgtcga ctgcttgctg
    16141 cctggcaaga gcgcggcatt atgctgccct tgtcggtaaa cctctcagcg ctgcaactga
    16201 tgcacccgaa tatggtggcg gatatgctgg aactgttaac ccgctatcgc attcagccgg
    16261 gaacactgat tctggaagtg acagaaagcc gacgtattga cgaccctcat gctgcggtgg
    16321 cagtcctccg tccgctgcgc aatgccggcg ttcgggtggc gctggatgat ttcggcatgg
    16381 gctatgcggg gctgcgtcag ctgcagcata tgaaatcgtt gccaatcgac gtactgaaaa
    16441 tcgacaaaat gtttgttgaa ggcttgccgg aagatagcag catgattgct gcaattatca
    16501 tgctggcgca gagcctgaac ttacaaatga ttgccgaagg cgtggagact gaagcacaac
    16561 gcgactggct ggcaaaagcg ggcgttggta ttgcccaggg cttccttttt gctcgcccac
    16621 tccctattga aatcttcgaa gagagttacc tggaagaaaa gtagctaccc caaactgatt
    16681 acaaaacttt aaaaagtgct ggtttgtgcg agccagctca aactttttaa cctttttgtt
    16741 tcaattatga tccaggtaca tttctgtgat gttgtctggg tgttatttta aggccgcagg
    16801 taccccataa ccttacaaga cctgtggttt tactaaagga caccctatga aaacctctct
    16861 gtttaaaagc ctttactttc aggtcctgac agcgatagcc attggtattc tccttggcca
    16921 tttctatcct gaaataggcg agcaaatgaa accgcttggc gacgggttcg ttaagctcat
    16981 taagatgatc atcgctcctg tcatcttttg taccgtcgta acgggcattg cgggcatgga
    17041 aagcatgaag gcggtcggtc gtaccggcgc agtcgcactg ctttactttg aaattgtcag
    17101 taccatcgcg ctgattattg gtcttatcat cgttaacgtc gtgcagcctg gtgccggaat
    17161 gaacgtcgat ccggcaacgc ttgatgcgaa agcggtagcg gtttacgccg atcaggcgaa
    17221 agaccagggc attgtcgcct tcattatgga tgtcatcccg gcgagcgtca ttggcgcatt
    17281 tgccagcggt aacattctgc aggtgctgct gtttgccgta ctgtttggtt ttgcgctcca
    17341 ccgtctgggc agcaaaggcc aactgatttt taacgtgatc gaaagtttct cgcaggtcat
    17401 cttcggcatc atcaatatga tcatgcgtct ggcacctatt ggtgcgttcg gggcaatggc
    17461 gtttaccatc ggtaaatacg gcgtcggcac actggtgcag ctggggcagc tgattatctg
    17521 tttctacatt acctgtatcc tgtttgtggt gctggtattg ggttcaatcg ctaaagcgac
    17581 tggtttcagt atcttcaaat ttatccgcta catccgtgaa gaactgctga ttgtactggg
    17641 gacttcatct tccgagtcgg cgctgccgcg tatgctcgac aagatggaga aactcggctg
    17701 ccgcaaatcg gtggtggggc tggtcatccc gacaggctac tcgtttaacc ttgatggcac
    17761 atcgatatac ctgacaatgg cggcagtgtt tatcgcccag gccactaaca gtcagatgga
    17821 tatcgtccac caaatcacgc tgttaatcgt gttgctgctt tcttctaaag gggcggcagg
    17881 ggtaacgggt agtggcttta tcgtgctggc ggcgacgctc tctgcggtgg gccatttgcc
    17941 ggtagcgggt ctggcgctga tcctcggtat cgaccgcttt atgtcagaag ctcgtgcgct
    18001 gactaacctg gtcggtaacg gcgtagcgac cattgtcgtt gctaagtggg tgaaagaact
    18061 ggaccacaaa aaactggacg atgtgctgaa taatcgtgcg ccggatggca aaacgcacga
    18121 attatcctct taatctcacc acttatgccc gcactccctt ccccgagtgc gggtgtttgc
    18181 gcataattga cccctgtgcc tgaacttacc acctgggtga gttcaggttt atttcacttc
    18241 ttccgtgaca ttttcatgtt cttgcggtct aacacgaagt gtttttaacg tcatttagac
    18301 tgaccgacaa cgggcagttt tttattacca ggatagttga tcaggggttc acatgcaggg
    18361 cacaaaaatt cgacttttag cgggcggttt gctgatgatg gccactgctg gctatgtgca
    18421 ggcagatgcg ctccagcctg atccagcatg gcaacagggg acgctttcca acggtttaca
    18481 gtggcaagtg ctgactacac cccagcgtcc cagcgatcgt gttgaaattc gcctgctggt
    18541 taataccggt tcgctcgccg aaagtacaca acagag
//
LOCUS       DAMZFT010000092        18533 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.092, whole genome shotgun sequence.
ACCESSION   DAMZFT010000092 DAMZFT010000000
VERSION     DAMZFT010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18533)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18533)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18533
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.092"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(<1..255)
                     /locus_tag="PN977_003381"
     CDS             complement(<1..255)
                     /locus_tag="PN977_003381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001093935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cons domain protein"
                     /protein_id="HDI6043385.1"
                     /translation="MNTLFNQPLKVVNAGLHSFADNIQHAGGSAIALNWQPPAQGDID
                     AGLDLASLLRHPLVENANQIAMTRYLEAQPVLVDVMLAKEA"
     gene            complement(252..1811)
                     /gene="fdrA"
                     /locus_tag="PN977_003382"
     CDS             complement(252..1811)
                     /gene="fdrA"
                     /locus_tag="PN977_003382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000865643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase FdrA"
                     /protein_id="HDI6043386.1"
                     /translation="MKVKYKVFSNLYQDSVSLMQISAQISKLPGIQQASVVMGTPNNL
                     EQLSDAGLGNEINASPNDLVIAVMGEEDICNEALVLAQQRLTSKPDDETDSGIKTPEK
                     VSLEMALEAEPEANLALISVPGDYAAAEAIKALNLGMNVMMFSDNVSIVQEKSIKTLA
                     RERQRIVMGPDCGTAIVNGIPLGFANVVKRGAIGVIGASGTGLQEVTCRIDQLGAGIS
                     QALGTGGHDLSEEIGGISMLFALDALAQDDETRVIVLISKPPSPIVARTILERAEACG
                     KPVVVNFLGANPHDLARPNITAATTLASAANIAVALLNDQPLPTIETEISCDDLTMLQ
                     NACQRLPAHRQAIRGVFAGGTFCYEAQLICQQKGFSAASNTPVAGNRALANIWQSEDH
                     TLIDMGDDDFTRGKPHPMIDPTLRNQRLLNELNDSHTAVVLFDLVLGYGASTTPASEL
                     LDQLSHIDMNNAPLLIAHVCGTEADPQIRSQQISALQNAGVIIASSNAQAALWASTVA
                     QTQLQKKGLNA"
     gene            complement(1906..2766)
                     /locus_tag="PN977_003383"
     CDS             complement(1906..2766)
                     /locus_tag="PN977_003383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001374637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
                     /protein_id="HDI6043387.1"
                     /translation="MVQVTLCASSLGYLFPQNETQDLRLHSAFKHAVNLYSDAGTFIT
                     LLCAQTYLNLPDAARVWLPECWDWRREIAHSDPIQLTPGLLRTPRFCVALENATLWQS
                     PFVGGMLTLEAFPLVFQHYPTMASQRLLFCLEHNVQSTLHLPDSLTHQGLAIMEHPDA
                     LERQVPQLIGFGKGLTPDGDDYLLGYLAALWLWQLPAPLADHQYRLQQVIDQHAHNTT
                     DISRHYLERALQGHFSEPICQLLAQLVGSASAMTIASCAEQVMQFGATSGVDCLAGML
                     HGFRTLNTIN"
     gene            complement(2772..3440)
                     /locus_tag="PN977_003384"
     CDS             complement(2772..3440)
                     /locus_tag="PN977_003384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HDI6043388.1"
                     /translation="MTQSIFQAQPFELPFDPRTTALVMIDMQRDFVEAGGFGEALGND
                     VSLVRTAIAPCTEVLAAARQKGIMVIHTREGHRADLSDCPPAKLTRGGKTFIGEPGPM
                     GRILVRGEAGHDIIPELYPVAGEPVIDKPGKGAFYQTDLHLILQNHGIKTLIVCGVTT
                     EVCVTTTVREANDRGYECIIPEDCVGSYFPEFQKYALEMIKAQGAIFGWVTDSKAIIA
                     GLEG"
     gene            complement(3697..4506)
                     /locus_tag="PN977_003385"
     CDS             complement(3697..4506)
                     /locus_tag="PN977_003385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HDI6043389.1"
                     /translation="MATTQQSGFAPAASPLASTIVQTPDDAIVAGFTSIPSQGDNMPA
                     YHARPRQSDGPLPVVIVVQEIFGVHEHIRDICRRLALEGYLAIAPELYFREGDPNDFA
                     DIPTLLSGLVAKVPDSQVLADLDHVASWASRNGGDVHRLMITGFCWGGRITWLYAAHN
                     PQLKAAVAWYGKLTGDKTLNSPKQPVDIATDLNAPVLGLYGGQDNSIPQESVETMRQA
                     LRAANAKAEIIVYPDAGHAFNADYRPSYHAESAKDGWQRMLEWFTQYGVKK"
     gene            4768..5532
                     /gene="udp"
                     /locus_tag="PN977_003386"
     CDS             4768..5532
                     /gene="udp"
                     /locus_tag="PN977_003386"
                     /EC_number="2.4.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001593007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine phosphorylase"
                     /protein_id="HDI6043390.1"
                     /translation="MSKSDVFHLGLTKNDLQGATLAIVPGDPDRVEKIAALMDKPVKL
                     ASHREFTTWRAELDGKPVIVCSTGIGGPSTSIAVEELAQLGIRTFLRIGTTGAIQPHI
                     NVGDVLVTTASVRLDGASLHFAPLEFPAVADFECTTALVEAAKSIGATTHVGVTASSD
                     TFYPGQERYDTYSGRVVRHFKGSMEEWQAMGVMNYEMESATLLTMCASQGLRAGMVAG
                     VIVNRTQQEIPNAETMKQTESHAVKIVVEAARRLLK"
     gene            complement(5566..7056)
                     /locus_tag="PN977_003387"
     CDS             complement(5566..7056)
                     /locus_tag="PN977_003387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001606539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HDI6043391.1"
                     /translation="MFESELLTQGFSTLLNNPQALLFATFGVMLGIVIGALPGLTATM
                     GVAILLPFTYGMEPVSGLLMICGVFFGGVYGGSITAILLKIPGTPAAAATAIDGYELT
                     KQGKAGLALSAATFSSFSGGTLSIIVLMFLSPVLASWALKFSASESFALATFGLSIIA
                     SISGESLIKGLIAGVGGLLIATIGLDPMGGFPRFTGGFVELMNVPFIPVMIGLFAASE
                     AFRSMEQNQQIRQGAKVAIGSLLLPWQTLRRIALTILRSSGLGVFIGMIPGAGADIAA
                     FVAYNETRRFSKTPENFGKGEIKAVASCEAGANGCTGGALLPMLTLGIPGDAVTAIML
                     GALTLQGMQPGPLMFTDHGDMVYTLFVGMIFCYFMLLVLGLLSLKVIGNVVKIPGNIL
                     TPMILALCVVGTYALNNSLFDVGIMLIAGVVGYFMQKGGYPASPVVLALIMGPMAESN
                     FRRALSLSGGSLDFLYTRPITLALLTLAAFTLLTPIIRKIMRLRRQ"
     gene            complement(7069..7575)
                     /locus_tag="PN977_003388"
     CDS             complement(7069..7575)
                     /locus_tag="PN977_003388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001596577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HDI6043392.1"
                     /translation="MNTKQSVAQLAVPHRKRLSSTMVVALLLCVVAGAVMINAADFPA
                     TAIETDPGASAFPTFYACALIVLAVLLVIRDLLQAKPASCANAQEKPAFRKTATGIAA
                     TAFYIVTMSYCGYLITTPVFLIVIMTLMGYRRWVLTPGIALLLTAILWLLFVEALQVP
                     LPVGTFFE"
     gene            complement(7592..8566)
                     /locus_tag="PN977_003389"
     CDS             complement(7592..8566)
                     /locus_tag="PN977_003389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HDI6043393.1"
                     /translation="MIKHLTLPKKMAAMVLLAGAALSIAPVQAASYPTKQIELVVPYA
                     AGGGTDLVARAFADAAKNHLPVSIGVINKPGGGGAIGLSEIAAARPGGYKIGLGTVEL
                     TTLPSLGMVRFKTSDFKPIARLNADPAAITVRSDAPWNSYEEFMTYAKANPGKVRIGN
                     SGTGAIWHLAAAALEDKTGAKFSHVPYDGAAPAITGLLGGHIEAVSVSPGEVINHVKG
                     GKLKTLVVMADERMKTMPDVPTLKEKGVDLSIGTWRGLIVSQKTPQDVVDVLAKAAKE
                     TAEEPAFQDALQKLNLNYAWLDAASFQTQISEQEKYFDELLTRLGLKK"
     gene            complement(8591..9220)
                     /locus_tag="PN977_003390"
     CDS             complement(8591..9220)
                     /locus_tag="PN977_003390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001116470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-6-phosphogalactonate aldolase"
                     /protein_id="HDI6043394.1"
                     /translation="MPFKTLLQKTGLVAILRGVKPDEIVAIGEKLYAAGFRLIEIPMN
                     SPEALQSISLLRDALPKDCLVGAGTVLSVEQVVAVKEAGGQIIVMPHCDTAVIRRARA
                     LGMYCAPGVATPTEAFAAIEHGANAIKLFPAEQITPEVTKAWRAVIPQSVPMLPVGGI
                     TPETMARYLSHGANGFGLGSALYHPGMTPEQVYENAVLFMNAWNNLNSN"
     gene            complement(9210..10214)
                     /locus_tag="PN977_003391"
     CDS             complement(9210..10214)
                     /locus_tag="PN977_003391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001117527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygalactonokinase"
                     /protein_id="HDI6043395.1"
                     /translation="MPGAPSFIALDWGTSSLRAWRFGESPNPQEKREFPWGIMKLPSQ
                     AATREDAFHDTFLRVCGDWLAQTPCPVLACGMLGSAQGWQPAAYLPCPVTLEGLAKQL
                     TPVIHQQQTMLHIIPGVIKEGEMPEVMRGEETQIFGAISMEPALQNAIHQGMPVLIGL
                     PGTHAKWAVVENNTITDFRTFMTGELFDVLSRHSILGATMHPGDEPHWDAFTHGLTAA
                     QEHHQTGLLSTLFSTRSRLLTSNLTSSSQGDYLSGLLIGHELCGLASSLLRDLPATTP
                     IALIGSANLNSRYSQAFSHVFPDRQIHAIPNATEQGLWRIAHAAGLLSTNARECTHAI
                     "
     gene            complement(10238..11005)
                     /locus_tag="PN977_003392"
     CDS             complement(10238..11005)
                     /locus_tag="PN977_003392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000852399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HDI6043396.1"
                     /translation="MAETSSTTAGAQTLLRGLAVLNAVYNGCHDLKSIGEFTGTTRST
                     THRLVTVLVEQRYLRHVPTQGYQLGAKLIEFGARALESTSLYEIAQPVLQRLARYTLD
                     TVHLGIVEGDEVLYLEKINSQRGLEMRSRPGHRMPLAITGIGKALILNRTEEEWRTLF
                     KTCGDETKLGAFIQNMRRYAASGFAFDLEENEPTIRCVAAPVYNARDEIVAAISVAST
                     TTYMSLARLEELAPYVKSCAEEISAELGWGKHVRKDK"
     gene            11222..12649
                     /gene="rmuC"
                     /locus_tag="PN977_003393"
     CDS             11222..12649
                     /gene="rmuC"
                     /locus_tag="PN977_003393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HDI6043397.1"
                     /translation="MDFSIMVYAVIALVGVAIGWLFASYQHAQQKAEQLAEREEMVAE
                     LSAAKQQITQSEHWRAECELLNNEVRSLQSINTSLEADLREVTTRMEAAQQHADDKIR
                     QMINSEQRLSEQFENLANRIFEHSNRRVDEQNRQSLNSLLSPLREQLDGFRRQVQDSF
                     GKEAQERHTLTHEIRNLQQLNAQMAQEAINLTRALKGDNKTQGNWGEVVLTRVLEASG
                     LREGYEYETQVSIENDARSRMQPDVIVRLPQGKDVVIDAKMTLVAYERYFNAEDDYTR
                     ESALQEHIASVRNHIRLLGRKDYQQLPGLRTLDYVLMFIPVEPAFLLALDRQPELITE
                     ALKNNIMLVSPTTLLVALRTIANLWRYEHQSRNAQQIADRASKLYDKMRLFVDDMSAI
                     GQSLDKAQDNYRQAMKKLSSGRGNVLAQAEAFRGLGVEIKREINPDLAEQAVSQDEEY
                     RLRSVPEQPNDEAYQRDDEYNQQSR"
     gene            12744..13499
                     /gene="ubiE"
                     /locus_tag="PN977_003394"
     CDS             12744..13499
                     /gene="ubiE"
                     /locus_tag="PN977_003394"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HDI6043398.1"
                     /translation="MVDKSQETTHFGFQTVAKEQKADMVAHVFHSVASKYDVMNDLMS
                     FGIHRLWKRFTIDCSGVRRGQTVLDLAGGTGDLTAKFSRLVGETGKVVLADINESMLK
                     MGREKLRNIGVIGNVEYVQANAEALPFPDNTFDCITISFGLRNVTDKDKALRSMYRVL
                     KPGGRLLVLEFSKPIIEPLSKAYDAYSFHVLPRIGSLVANDADSYRYLAESIRMHPDQ
                     DTLKAMMQDAGFESVDYYNLTAGVVALHRGYKF"
     gene            13513..14118
                     /gene="ubiJ"
                     /locus_tag="PN977_003395"
     CDS             13513..14118
                     /gene="ubiJ"
                     /locus_tag="PN977_003395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis protein UbiJ"
                     /protein_id="HDI6043399.1"
                     /translation="MPFKPLVTAGIESLLNTFLYRSPALKTARSRLLGKVLRVEVKGF
                     STSLILVFSERQVDVLGEWAGDADCTVIAYASVLPKLRDRQQLAALIRSGELEVQGDI
                     QVVQNFVALADLAEFDPAELLAPYTGDIAAEGISKALRGGAKFLHHGIKRQQRYVAEA
                     ITEEWRMAPGPLEVAWFAEETAAVERAVDALTKRLEKLEAK"
     gene            14115..15755
                     /gene="ubiB"
                     /locus_tag="PN977_003396"
     CDS             14115..15755
                     /gene="ubiB"
                     /locus_tag="PN977_003396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis regulatory protein
                     kinase UbiB"
                     /protein_id="HDI6043400.1"
                     /translation="MTPGEVRRLYFIIRTFLSYGLDELIPKMRITLPLRLWRYSLFWM
                     PNRHKDKPLGERLRLALQELGPVWIKFGQMLSTRRDLFPPHIADQLALLQDKVAPFDG
                     KLAKQQIEAAMGGLPVEAWFDDFEIKPLASASIAQVHTARLKSNGKEVVIKVIRPDIL
                     PVIKADLKLIYRLARWVPRLLPDGRRLRPTEVVREYEKTLIDELNLLRESANAIQLRR
                     NFEDSPMLYIPEVYPDYCSEGMMVMERIYGIPVSDVATLEKNGTNMKLLAERGVQVFF
                     TQVFRDSFFHADMHPGNIFVSYEHPENPKYIGIDCGIVGSLNKEDKRYLAENFIAFFN
                     RDYRKVAELHVDSGWVPPDTNVEEFEFAIRTVCEPIFEKPLAEISFGHVLLNLFNTAR
                     RFNMEVQPQLVLLQKTLLYVEGVGRQLYPQLDLWKTAKPFLESWIKDQVGIPALVRAF
                     KEKAPFWVEKMPELPELVYDSLRQGKYLQHSVDKIARELQSNHVRQGQSRYFLGIGAT
                     LVLSGTFLLVSRPEWGLMPVWLMAGGLIAWFVGWRKTR"
     gene            15834..16103
                     /gene="tatA"
                     /locus_tag="PN977_003397"
     CDS             15834..16103
                     /gene="tatA"
                     /locus_tag="PN977_003397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatA"
                     /protein_id="HDI6043401.1"
                     /translation="MGGISIWQLLIIAVIVVLLFGTKKLGSIGSDLGASIKGFKKAMS
                     DDEPKQDKTSQDADFTAKTIADKQADTNQEQAKTEDAKRHDKEQV"
     gene            16107..16622
                     /gene="tatB"
                     /locus_tag="PN977_003398"
     CDS             16107..16622
                     /gene="tatB"
                     /locus_tag="PN977_003398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatB"
                     /protein_id="HDI6043402.1"
                     /translation="MFDIGFSELLLVFIIGLVVLGPQRLPVAVKTVAGWIRALRSLAT
                     TVQNELTQELKLQEFQDSLKKVEKASLTNLTPELKASMDELRQAAESMKRSYVANDPE
                     KASDEAHTIHNPVVKDNEAAHEGVTPAAAQTQASSPEQKPETTPEPVVKPAADAEPKT
                     AAPSPSSSDKP"
     gene            16625..17401
                     /gene="tatC"
                     /locus_tag="PN977_003399"
     CDS             16625..17401
                     /gene="tatC"
                     /locus_tag="PN977_003399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatC"
                     /protein_id="HDI6043403.1"
                     /translation="MSVEDTQPLITHLIELRKRLLNCIIAVIVIFLCLVYFANDIYHL
                     VSAPLIKQLPQGSTMIATDVASPFFTPIKLTFMVSLILSAPVILYQVWAFIAPALYKH
                     ERRLVVPLLVSSSLLFYIGMAFAYFVVFPLAFGFLANTAPEGVQVSTDIASYLSFVMA
                     LFMAFGVSFEVPVAIVLLCWMGITSPEDLRKKRPYVLVGAFVVGMLLTPPDVFSQTLL
                     AIPMYCLFEIGVFFSRFYVGKGRNREEENDAEAESEKTEE"
     gene            17443..18225
                     /gene="tatD"
                     /locus_tag="PN977_003400"
     CDS             17443..18225
                     /gene="tatD"
                     /locus_tag="PN977_003400"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' ssDNA/RNA exonuclease TatD"
                     /protein_id="HDI6043404.1"
                     /translation="MFDIGVNLTSSQFAKDRDDVVARAFDAGVNGLLITGTNLRESQH
                     AQKLARQYSSCWSTAGVHPHDSSQWQAATEEAIIELAAQPEVVAIGECGLDFNRNFST
                     PEEQERAFVAQLRIAAELNMPVFMHCRDAHERFMTLLEPWLDKLPGAVLHCFTGTREE
                     MQACVARGIYIGITGWVCDERRGLELRKLLPLIPAEKLLIETDAPYLLPRDLTPKPSS
                     RRNEPAHLPHILQRIAHWRGEDAAWLAATTDANVKTLFGIAF"
     gene            complement(18222..>18533)
                     /gene="rfaH"
                     /locus_tag="PN977_003401"
     CDS             complement(18222..>18533)
                     /gene="rfaH"
                     /locus_tag="PN977_003401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription/translation regulatory transformer
                     protein RfaH"
                     /protein_id="HDI6043405.1"
                     /translation="DPEVIHTTTINATRGVSHFVRFGASPAIVPSAVIHQLSVYKPKD
                     IVDPATPYPGDKVIITEGAFEGFQAIFTEPDGEARSMLLLNLINKEIKHSVKNTEFRK
                     L"
BASE COUNT         4467 a         4666 c         4917 g         4483 t
ORIGIN      
        1 ggcctcttta gcgagcatga catccaccag cacgggctgt gcttcgagat aacgtgtcat
       61 ggcgatttgg ttggcatttt ctaccagggg atgacgtagc aacgatgcca gatccaatcc
      121 agcgtcaata tcgccttgtg ctggtggctg ccagttgagt gcaattgcgc ttcccccggc
      181 atgttgaata ttgtcagcaa aactgtgtag tcccgcgttc acaaccttca aaggctgatt
      241 aaataaggta ttcatgcgtt tagccccttt ttctgcaact gagtctgtgc aacggtgctg
      301 gcccataacg ccgcctgtgc attgctgctg gcgatgatga cccctgcatt ttgtagtgcg
      361 cttatctgct gactgcgtat ttgcggatca gcttccgttc cgcaaacgtg cgcaatcagc
      421 aacggtgcgt tattcatgtc gatgtgtgaa agttggtcca gtaattcgct ggcaggcgtc
      481 gtggaagcgc cataaccaag taccaggtcg aataacacga cggcagtgtg tgagtcgttg
      541 agttcgttga gcagacgttg attgcgcaga gtcgggtcga tcattggatg cggtttgcca
      601 cgggtgaaat catcgtcgcc catatcgatc aacgtgtgat cttcgctttg ccagatattt
      661 gccagagcac gattacccgc aactggcgta ttggaggctg cgctgaatcc cttttgttgg
      721 cagataagtt gcgcttcgta gcagaaggtt cctccggcga aaacaccgcg tatcgcctgg
      781 cgatgggctg gcaggcgctg gcaggcgttt tgtaacattg tgagatcgtc gcaggaaatt
      841 tcggtctcga tagtcggaag tggttgatca ttcagtaacg ccacggcgat atttgcggcg
      901 ctggcaagtg tggtggccgc ggtgatattg ggacgtgcca gatcatgcgg attcgcaccg
      961 aggaaattca ccaccacggg ttttccacaa gcctcggcac gctccagaat agtccgggca
     1021 acgatcggtg aaggcggttt tgaaataagc acaatcacgc gagtttcatc gtcttgcgcc
     1081 agcgcatcta aagcgaagag catcgatatt ccaccaatct cttcactgag atcatgtccg
     1141 cctgttccca acgcctgaga aatacccgct ccgagctggt cgattcggca agtcacttcc
     1201 tgtagcccgg tgcctgacgc gccaataaca ccaattgctc cgcgtttcac cacattagcg
     1261 aagcctaacg gaatgccgtt aacaatggcg gttccacagt caggtcccat tacgatgcgc
     1321 tgacgttctc gggcaagcgt tttgatggat ttttcctgca cgatactgac gttatcgctg
     1381 aacatcatca cgttcattcc gaggtttaac gctttaattg cttcggcggc ggcgtagtct
     1441 cccggtacag aaatgagtgc caggttggct tcgggctcgg cttccagtgc catttccagg
     1501 ctgacctttt ccggtgtttt aatgccacta tccgtttcat catcgggttt gctggtcaga
     1561 cgttgctggg ccagtaccag tgcttcattg caaatatctt cttcacccat gactgcaatc
     1621 actaaatcat tgggactggc gttgatttcg ttacccagac ccgcatcgct caattgctcg
     1681 agattattgg gtgttcccat gacgaccgag gcctgttgaa taccgggcaa cttgctgatt
     1741 tgcgcggaaa tctgcatcag agaaactgag tcctgataaa gattactgaa aactttatat
     1801 ttaactttca tgctgttacg cctgtttcat tccgcccgat agcaaaattc gggcaaagca
     1861 tccccgtgta gccccataaa cgggctacgg gagaaaaggt tgaaatcaat ttatggtgtt
     1921 cagggttcga aagccatgca gcattcctgc aaggcaatct actcctgacg ttgcgccaaa
     1981 ttgcatgacc tgttctgcac aggatgcgat tgtcattgcc gatgcactcc caaccagttg
     2041 tgcgagtaac tggcaaatcg gttctgaaaa atgtccctga agcgcacgct ccagataatg
     2101 acgactgata tccgtggtgt tgtgcgcatg ttgatcaatt acctgctgca accggtattg
     2161 atgatcggca agtggtgcag ggagttgcca taaccagagt gctgccagat agcccaacaa
     2221 atagtcatct ccatcgggcg ttaagccttt accaaagccg atcagttgtg gcacctggcg
     2281 ttccagcgca tctggatgct ccataatcgc taatccctgg tgcgtgagac tatccggcag
     2341 gtgcaatgtg ctttgaacgt tatgttccag acaaaataaa agccgttgcg atgccatcgt
     2401 tgggtagtgc tggaaaacca aagggaatgc ctcaagtgtc agcattcccc ccacaaatgg
     2461 cgactgccag agcgttgcgt tttcgagcgc cacacaaaat cgaggtgtcc gcagtaagcc
     2521 tggggttaac tgaatgggat ctgaatgggc aatctctctt cgccagtccc aacattcagg
     2581 tagccagact ctggctgcat cagggagatt cagatacgtt tgcgcacaca acagagtgat
     2641 gaatgttccc gcgtctgaat acagatttac cgcatgttta aaagcgctat gtagacgtaa
     2701 atcttgcgtt tcattctgcg ggaaaagata tcccaacgac gatgcgcaaa gcgtaacctg
     2761 taccatcggt gttagccttc caggcctgca ataatggctt tggaatcagt aacccaaccg
     2821 aaaatggcac cttgtgcttt gatcatctcg agggcgtatt tctggaattc cgggaaatag
     2881 gagccgacac aatcttccgg gataatgcat tcatagccac gatcatttgc ttcgcgcacg
     2941 gtggtggtta cgcaaacttc ggttgtaaca ccacaaacta tcagcgtctt gataccgtgg
     3001 ttttgcaaaa tcagatgtaa gtcggtttga taaaacgcgc ctttgccagg cttatcaatc
     3061 accggttcgc ctgccaccgg gtacagttca ggaatgatgt catgacctgc ttcacctctg
     3121 accagaatgc gtcccatcgg tcctggttca ccaataaagg tttttccgcc gcgcgtcagt
     3181 ttggctggcg ggcagtcgct taagtctgca cgatggcctt cacgagtatg aataaccata
     3241 attccttttt gacgtgcggc tgccagcacc tctgtacatg gtgcgatagc cgtgcgtacc
     3301 agtgaaacat cattgcccag tgcctcaccg aagccccctg cttcgacaaa gtcacgctgc
     3361 atgtcgatca tgaccagtgc agttgtgcgc ggatcaaaag gtaattcaaa gggttgagcc
     3421 tgaaaaatgg attgcgtcat ggtaaatctc ctgtctgatg tttaaacgat ccagttgttc
     3481 tgagaacgag tattcaggag aaaagcggca caaaacagga aaaaagtttg ccgcacagca
     3541 acgaaatttt tgcattcagc aggtggtgtg atgacgaatg tgaagggagt caaagagtgg
     3601 catacagcaa caaatttttt gatgctgtat ggttttgaga ttaaaaaaat cgttgaaatt
     3661 gtgtgagctt aatcatcaga tttagcgact atggcgttac tttttaacac catactgtgt
     3721 aaaccattcc aacattcgct gccagccgtc tttcgcagac tcggcatgat agctcggacg
     3781 ataatcggcg ttgaacgcat gccctgcatc cgggtacacg ataatctctg ctttcgcgtt
     3841 tgctgcccgc agcgcctggc gcatcgtttc cacactctct tgcggaatgc tgttatcctg
     3901 accaccatat aagccgagaa ccggcgcgtt aagatcggtt gcgatatcaa caggttgttt
     3961 cggtgaattc agcgtcttat cgccggttaa tttgccgtac catgccaccg ctgctttcag
     4021 ttgtggatta tgcgcggcat acagccaggt gatacgtcca ccccagcaga atccggtgat
     4081 cattaaacga tgaacatcgc cgccgttgcg ggaagcccaa ctggcgacat gatcgagatc
     4141 ggccagcacc tgcgagtcag gcacttttgc taccagaccg ctaagcagcg tagggatatc
     4201 ggcaaaatca ttcggatcgc cttcgcggaa gtaaagttca ggtgcgatag ccagataccc
     4261 ctccagcgcc agacggcgac aaatatcgcg gatatgttca tgcacgccaa aaatttcctg
     4321 cactacaatg accactggca gtgggccatc gctttgcctt ggtctggcat ggtaagcagg
     4381 catgttatcc ccttgtgaag ggatagaggt gaagcccgcc acaatcgcgt cgtccggagt
     4441 ctgaacgatg gtcgaagcga gaggcgatgc agcaggtgca aatccagatt gttgtgttgt
     4501 tgccatggta ttctccgtac ccttataaaa atgttgcgca atgttaacta tagtcagcat
     4561 gcaacaaatc acattgcctg aatcggctca tcttttatgc agtcctgcag aatgaagagt
     4621 gatttatgtg atttgcatca cttttggcgg gtaaatttat gcaacgcatt ttcgtcatgg
     4681 tgatgagtat cacgaaaaaa tgttaaaccc ttcggtaaag tgtctttttg cttcttctga
     4741 ctaaaccgat tcacagagga gttgtatatg tccaagtctg atgtttttca tctcggcctc
     4801 actaaaaacg atttacaagg ggctacgctt gccatcgtcc ctggcgaccc ggatcgtgtg
     4861 gaaaagatcg ccgcgctgat ggataagccg gttaagctgg catctcatcg cgaattcacc
     4921 acctggcgtg cagagctgga tggtaaacct gttatcgtct gctctaccgg tatcggcggc
     4981 ccgtctacct ctattgctgt tgaagagctg gcacagctgg gcattcgcac cttcctgcgt
     5041 atcggcacaa caggcgctat tcagccgcat attaatgttg gcgatgtcct ggttaccacg
     5101 gcgtctgttc gtctggatgg tgcgagcctg cacttcgcac cgctggaatt cccggctgtc
     5161 gctgatttcg aatgtacgac tgcgctggtt gaagcggcga agtcaattgg tgccaccact
     5221 cacgttggtg tgacggcttc ttctgatacc ttctacccgg gtcaggaacg ttacgatact
     5281 tactctggtc gcgtagttcg tcactttaaa ggttctatgg aagagtggca ggcgatgggc
     5341 gtaatgaact atgaaatgga atctgcaacc ctgctgacca tgtgtgcaag tcagggcctg
     5401 cgtgccggta tggtagcggg ggttatcgtt aaccgcaccc agcaagagat cccgaatgct
     5461 gagacgatga aacaaaccga aagccatgcg gtgaaaatcg tggtggaagc ggcgcgtcgt
     5521 ctgctgaaat aaaaaacgct ccccaacttc ggggagcgat atcagttatt gacgccgtaa
     5581 acgcattatt ttgcggatta ttggcgtcag tagcgtaaag gctgccagag tcagcaatgc
     5641 cagagttatc ggtcgggtat acagaaagtc gagtgaccca ccagaaagcg acagcgcacg
     5701 gcgaaaatta ctttccgcca ttggccccat aatcaatgcc agcactacgg gtgatgccgg
     5761 atatcctcct ttctgcatga aatagcccac cacgcctgca atcagcataa tgccaacatc
     5821 aaacaggcta ttgttcaacg cataagtccc gaccacacaa agtgcgagga tcatcggtgt
     5881 gagaatattg ccgggaattt tcaccacatt accgatgact ttcaaagaga gcagtccaag
     5941 aactagtagc atgaagtaac agaagatcat gccgacaaac agtgtataaa ccatatcgcc
     6001 gtggtcggta aacatcagcg ggcctggttg cattccctgt aacgttaacg cgcccagcat
     6061 gatggcggtt accgcatcgc cagggatccc cagcgtcagc ataggtaaca atgcgccacc
     6121 ggtgcagcca ttcgcgcctg cctcacagga ggccacagct ttaatttcac ccttaccaaa
     6181 gttttctggt gtcttactga aacgacgagt ttcgttatag gcaacaaaag ctgcaatatc
     6241 cgcccccgca ccggggatca tgccgataaa aacccctaat cctgatgagc gcaaaatggt
     6301 taatgcaatg cggcgtagtg tttgccaggg cagtaacaga ctgccgatag ccaccttcgc
     6361 cccctgacga atttgctggt tttgctccat agagcggaat gcttctgaag cagcaaataa
     6421 accaatcatc accgggataa atggcacatt catcagctcc acaaatccac ctgtaaaccg
     6481 tggaaaaccg cccattggat caaggcctat cgttgcgatc agcaatccgc caaccccggc
     6541 aatcagccct ttaatcagtg actcaccgga aatactggca ataatgctca gtccgaaggt
     6601 tgccagggcg aaggactccg aggcactaaa tttcagcgcc caactggcaa gtaccggaga
     6661 gagaaacatc agcacgataa tgctgagcgt tccgccacta aaggaagaga acgtggcggc
     6721 actcaatgcc agccctgctt ttccctgttt cgtcagctca taaccatcaa tagcggtggc
     6781 tgcggcggca ggcgttccgg gaattttgag caaaattgcg gtgatagaac caccgtagac
     6841 gccaccaaaa aagacgccgc aaatcatcaa caagccagaa acaggctcca tgccgtaggt
     6901 gaaaggcagc aaaatcgcca cacccatggt cgctgtcaga ccaggcaaag cgccaatcac
     6961 aatacccagc atcaccccaa aagtggcaaa cagcagcgcc tgaggattat tgagtaatgt
     7021 cgaaaaaccc tgagttaaaa gttcactttc aaacattgct ggcccctgtt attcgaaaaa
     7081 tgtgccgaca ggcaatggca cctgtaacgc ttcgacaaac agcaaccaga ggattgccgt
     7141 taacagcagc gcaatacccg gtgtgagtac ccatcgcctg tagcccatca acgtcataat
     7201 gacgatgagg aaaacaggag tagtaatgag ataaccgcag tagctcatcg tcacaatata
     7261 aaacgcggtt gccgcaattc ctgttgccgt tttcctgaat gccggttttt cctgtgcgtt
     7321 ggcgcaagag gctggttttg cctgcaaaag atcgcgtatc accagcaaga cagcgagcac
     7381 aatcagggca caggcataga aggtagggaa ggcacttgca ccgggatccg tttcaatggc
     7441 agttgctgga aaatcagcgg cattaatcat caccgcgcca gcaaccacac aaagtaacag
     7501 cgccaccacc atcgttgatg aaaggcgctt gcggtgcggt acggcgagtt gagcaacaga
     7561 ctgttttgtg ttcatcaacc gtacctcagg tttatttttt caggcccagg cgagtcagca
     7621 actcgtcaaa gtacttttcc tgttcgctga tttgggtctg gaagctggca gcgtcaagcc
     7681 atgcatagtt gagattcaac ttttgcagtg catcctggaa tgcaggctct tcagccgtct
     7741 cttttgctgc ctttgccaga acatccacca catcctgcgg cgttttttgc gacacaatca
     7801 ggccgcgcca ggtgccgatg gagagatcaa cgcctttctc ttttaacgtc gggacgtcag
     7861 gcatggtttt cattcgctca tccgccatca ctaccagtgt cttcagcttg ccgcctttca
     7921 catggttgat aacttctcct gggcttacgg aaaccgcttc aatatgcccg cctaacaggc
     7981 ctgtaatggc aggggctgcg ccgtcatacg ggacatgaga aaacttcgca cccgttttgt
     8041 cttccagtgc agctgccgcc agatgccaga tagctccggt gcctgagtta ccaatgcgta
     8101 cttttccggg attcgctttg gcgtaagtca taaattcttc atagctattc cacggcgcat
     8161 cagaacggac ggtgatagca gccggatccg cgtttagacg ggcaatgggt ttaaaatcgc
     8221 tggttttaaa acgcaccatt ccgaggctgg gaagggtggt cagttcaacc gtgcctaaac
     8281 caattttgta accgccaggg cgggcagcgg cgatttcact caggccgata gcaccgcctc
     8341 cgccaggttt attgataacc ccgatgctga cgggtaaatg gtttttggcg gcatcagcaa
     8401 aggcacgggc aaccagatcc gtaccgcctc cggcagcgta gggaacgact aactcgatct
     8461 gtttggttgg gtaggaggct gcctgtacag gcgcaatgct gagggcagcg cctgcaagaa
     8521 gtaccattgc agccattttt ttcggtaagg taagatgttt tatcattgct tttatatcct
     8581 tatttatcag ttagttgcta tttaaattgt tccatgcgtt cataaacagt actgcatttt
     8641 catagacttg ctctggcgtc atgccggggt gatatagcgc agaaccgagg ccaaagccgt
     8701 ttgcgccgtg gctcagataa cgcgccatgg tttccggtgt gataccgcca accggtaaca
     8761 tcggaacgga ttgtggaata accgcacgcc aggctttggt aacttctggc gtaatttgtt
     8821 cagccgggaa cagcttgatg gcattagcac catgttcaat ggcagcaaag gcttcggtgg
     8881 gcgtggcgac gccaggcgca caatacatac ccagcgcacg ggcgcgacgg atcaccgcag
     8941 tatcgcaatg cggcatcacg ataatctggc ccccagcctc tttaaccgca accacctgct
     9001 ctaccgaaag cacagttccg gctccgacca ggcaatcctt aggcagggcg tcgcgcagta
     9061 aactgatact ttgcaacgcc tccggcgagt tcataggaat ctcaatcagg cggaatccgg
     9121 cggcgtacag tttttcaccg atggcgacaa tctcatccgg tttcacgccg cgtaaaatgg
     9181 cgactaaccc ggttttttgc aaaagcgtct taaatggcat gggtacattc cctggcgttg
     9241 gtggacagta acccggcagc gtgggcgatt cgccataatc cctgttcagt ggcattagga
     9301 atggcgtgta tctgtctgtc gggaaaaacg tggctgaatg cttgtgaata gcggctgttc
     9361 agatttgcgc taccgattaa ggcgatcggt gttgtggcgg gtaaatcacg tagcaaactg
     9421 gatgccagac cgcataattc atggcctatc agtaatccgg aaaggtaatc cccctgcgag
     9481 gacgacgtaa gattactggt cagcaggcgc gaacgggtcg aaaacagcgt cgataataat
     9541 ccggtctgat gatgctcttg tgctgctgtc agcccgtggg tgaaggcatc ccagtgcggt
     9601 tcatctccag gatgcatggt ggcaccgaga atcgaatggc gggataaaac atcaaataac
     9661 tcgcccgtca taaaggttcg gaaatcggta atggtgttgt tttcaactac tgcccatttc
     9721 gcatgtgtgc cgggtaagcc tatcagcact ggcataccct gatggatcgc gttttgcagg
     9781 gccggttcca tcgagatagc accgaagatt tgtgtctctt cgccacgcat cacttcgggc
     9841 atttcacctt ctttaatcac gccaggaatg atgtgcagca ttgtctgctg ctggtggata
     9901 actggcgtca actgcttcgc cagaccctcg agagtgaccg ggcaaggtaa ataagctgct
     9961 ggttgccacc cttgcgcact gccgagcatg ccgcaagcca gtaccgggca tggggtttgt
    10021 gccagccagt cgccgcagac acgtaaaaac gtatcgtgga aagcgtcctc acgtgttgct
    10081 gcctgggagg gtaatttcat gatcccccag gggaattcac gtttttcttg tggatttggt
    10141 gattcgccaa aacgccatgc ccgaagcgat gacgttcccc agtccagggc aataaatgag
    10201 ggtgctcccg gcattagtcg tttcctttaa tcatggttta tttatctttg cggacgtgct
    10261 taccccagcc cagttcagcg gatatctctt ctgcacagga tttgacgtaa ggtgccagtt
    10321 cttccagacg cgccagcgac atatacgtgg tggtactggc gacagaaata gccgccacaa
    10381 tttcatcccg ggcgttgtaa accggcgctg caacgcaacg gatagtcggc tcgttctctt
    10441 ccagatcaaa agcaaaacca ctggcggcat aacgacgcat gttctgaata aatgcgccca
    10501 gttttgtttc atcaccacag gtcttaaaca gcgtgcgcca ctcttcttct gttcggttga
    10561 gtatcaatgc tttgccgatg ccggtgattg ccagtggcat gcgatggcct gggcgtgagc
    10621 gcatttccag cccgcgctga ctgttgatct tctccagata cagcacttcg tcgccttcaa
    10681 cgatccccag atgcacggtg tccagggtgt aacgggcaag gcgttgcaaa acgggctggg
    10741 caatttcgta cagcgaggtg ctttccagtg ctctggcacc gaattcgatc agtttggcac
    10801 ccagttggta gccctgcgtt gggacatggc gcaggtagcg ttgttccacc agaacggtga
    10861 ctaaacgatg ggttgtgctc cgggtcgtgc cggtgaactc gccgatactt tttaggtcat
    10921 ggcagccgtt ataaacagca ttaagaaccg ccagtccacg taataacgtt tgtgcgcctg
    10981 cggtggttga agaggtttct gccatacgtt tctcctgatg atattgctga agtgttccac
    11041 ataatgacgt ggggaaaagg tgatatatcc cacaatataa actccatctc atattgtggg
    11101 ataaaataat gaaaattaac gatcgttttg cgtagcgcct cgcaggaaac ttctttcaaa
    11161 ctggacgttt gtacagcaca attctatttt gtgcgggtaa gttgttgcgt caggaggcgt
    11221 tgtggatttc tcaatcatgg tttacgcagt tattgcattg gtgggtgtgg caattggctg
    11281 gctgtttgcc agttatcaac atgcgcagca aaaagccgag caattagctg aacgtgaaga
    11341 gatggtcgcg gagttaagcg cggcaaaaca acaaattact caaagcgagc actggcgcgc
    11401 tgagtgcgag ttactcaata acgaagtgcg cagcctgcaa agtattaaca cctctctgga
    11461 ggccgatctg cgtgaagtaa ccacgcggat ggaagctgca cagcaacatg ctgacgataa
    11521 aattcgccag atgattaaca gcgagcagcg cctcagtgag cagtttgaaa acctcgccaa
    11581 ccgtattttt gagcacagca atcgccgggt tgatgagcaa aaccgtcaga gcctgaacag
    11641 cctgttgtcg ccgctacgtg aacaactgga cggtttccgc cgtcaggtcc aggacagctt
    11701 tggtaaagaa gcccaagaac gtcataccct gactcacgaa atccgcaacc tccagcaact
    11761 caacgcccag atggcccagg aagcgatcaa cctgacgcgt gcgctgaaag gcgacaataa
    11821 aacccagggc aactggggcg aggtagtatt gacgcgggtg ctggaggctt ccggtctgcg
    11881 tgaagggtat gaatatgaaa cccaggtcag catcgaaaat gacgcccgct cgcggatgca
    11941 gccggatgtc atcgtgcggt tgccgcaggg aaaagatgtg gtgatcgacg ccaaaatgac
    12001 gctggtcgcc tatgaacgct atttcaacgc cgaagatgac tacacccgcg aaagcgcgct
    12061 acaggaacat atcgcgtcgg tgcgtaacca tatccgtttg ctgggacgca aagattatca
    12121 acagctgccg gggctgcgaa ccctggatta cgttctgatg tttattcccg ttgaacccgc
    12181 ttttttactg gcgcttgacc gccagccgga gctgatcacc gaagcgttga aaaacaatat
    12241 catgctggtt agcccgacta cgctgctggt ggcgctgcgc actatcgcca acctgtggcg
    12301 ttatgagcat caaagccgca acgcccagca aatcgccgat cgtgccagca agctgtacga
    12361 caagatgcgt ttgttcgtcg atgacatgtc agcgattggt caaagtctcg acaaagcgca
    12421 ggataactac cgccaggcaa tgaaaaaact ctcttcaggt cgcggaaatg tgctggcgca
    12481 ggcagaagcg tttcgcggtt taggggtaga aattaaacgc gagattaatc cggatttggc
    12541 tgaacaagcg gtgagccagg atgaagagta tcgactgcgg tcggttccgg agcagccgaa
    12601 tgatgaagct tatcaacgcg atgatgaata taatcagcag tcgcgctagc ccattggggg
    12661 tagttaagcc gggtagaaat ctagggcatc gacgcccaat ctgttacact tctggaacaa
    12721 ttttttgatg agcaggcatt gagatggtgg ataagtcaca agaaacgacg cactttggtt
    12781 ttcagaccgt cgcgaaggaa caaaaagcgg atatggtcgc tcacgttttc cattccgtgg
    12841 catcaaaata cgatgtcatg aatgatttga tgtcattcgg tattcatcgt ttgtggaagc
    12901 gattcacgat tgattgcagc ggcgtacgcc gtgggcagac cgtgctggat ctggctggtg
    12961 gcaccggcga cctgacagcg aaattctccc gcctggtcgg agaaactggc aaagtggtcc
    13021 ttgctgatat caatgaatcc atgctcaaaa tgggacgcga gaagctgcgt aatatcggtg
    13081 tgattggcaa cgttgagtat gttcaagcga acgctgaggc gctgccgttc ccggataaca
    13141 cctttgattg catcaccatt tcgtttggtc tgcgtaacgt caccgacaaa gataaagcac
    13201 tgcgttcaat gtatcgcgtg ctgaaacccg gcggccgcct gctggtgctt gagttctcga
    13261 agccaattat cgagccgctg agcaaagcct atgatgcata ctccttccat gtgctgccgc
    13321 gtattggctc actggtcgcg aacgacgccg acagctaccg ttatctggca gaatccatcc
    13381 gtatgcatcc cgatcaggat accctgaaag ccatgatgca ggatgccgga ttcgaaagtg
    13441 tcgactacta caatctgacg gcaggggttg tggcgttgca tcgtggttat aagttctgac
    13501 aggagaccgg aaatgccttt taaaccttta gtgacggcag gaattgaaag tctgctcaac
    13561 accttcctgt atcgctcacc cgcgctgaaa acggcccgct cgcgtctgtt gggcaaagtg
    13621 ctgcgcgtgg aggtaaaagg cttttcgacg tcattgattc tggtgttcag cgaacgccag
    13681 gttgatgtac tgggcgaatg ggcaggcgat gctgactgca ccgttatcgc ctacgccagt
    13741 gtgttgccga aacttcgcga tcgccagcag cttgccgcac tgattcgcag tggtgagctg
    13801 gaagtgcagg gcgatattca ggtggtgcaa aacttcgttg cgctggcaga tctggcagag
    13861 ttcgaccctg cggaactgct ggccccttat accggtgata tcgccgctga aggtatcagc
    13921 aaagccctgc gcggaggcgc aaagttcctg catcacggca ttaagcgcca gcaacgttat
    13981 gtggcggaag ccattactga agagtggcgt atggcacccg gtccgcttga agtggcctgg
    14041 tttgcggaag agacggctgc cgtcgagcgt gctgttgatg ccctgaccaa acggctggaa
    14101 aaactggagg ctaaatgacg ccaggtgaag tacggcgcct atatttcatc attcgcactt
    14161 ttttaagcta cggacttgat gaactgatcc ccaaaatgcg tatcaccctg ccgctacggc
    14221 tatggcgata ctcattattc tggatgccaa atcggcataa agacaaacct ttaggtgagc
    14281 gactacgact ggccctgcaa gaactgggac cggtatggat caagttcggg cagatgttat
    14341 caacccgccg cgatcttttt ccgccgcata ttgccgatca gctggcgtta ttgcaggaca
    14401 aagtcgctcc gtttgatggc aagctggcga agcagcagat tgaagctgca atgggcggct
    14461 tgccggtaga agcgtggttt gacgattttg aaatcaagcc gctggcttct gcttctatcg
    14521 cccaggttca taccgcgcga ttgaaatcga atggtaaaga ggtggtgatt aaagtcatcc
    14581 gcccggatat tttgccggtt attaaagcgg atctgaaact tatctaccgg ctggctcgct
    14641 gggtgccgcg tttgctgccg gatggtcgcc gtctgcgccc aaccgaagtg gtgcgcgagt
    14701 acgaaaagac cttgattgat gaactgaatt tgctgcggga atctgccaac gccattcagc
    14761 ttcgacgcaa ttttgaagac agcccgatgc tctacatccc ggaagtttac cctgactatt
    14821 gtagtgaagg gatgatggta atggagcgta tttacggcat tccggtgtct gatgttgcga
    14881 cgctggagaa aaacggcaca aacatgaaat tgctggcgga acgcggcgtg caggtgttct
    14941 tcactcaggt ctttcgcgac agctttttcc atgccgatat gcaccctggc aacatcttcg
    15001 taagctatga acacccggaa aacccgaaat atatcggcat tgattgcggg attgttggct
    15061 cgctaaacaa agaagataaa cgctatctgg cagaaaactt tatcgccttc tttaatcgcg
    15121 actatcgcaa agtggcagag ctacacgtcg attctggctg ggtgccacca gataccaacg
    15181 ttgaagagtt cgaatttgcc attcgtacgg tctgtgaacc tatctttgag aaaccgctgg
    15241 ccgaaatttc gtttggacat gtactgttaa atctgtttaa tacggcgcgt cgcttcaata
    15301 tggaagtgca gccgcaactg gtgttactcc agaaaaccct gctctacgtc gaaggggtag
    15361 gacgccagct ttatccgcaa ctcgatttat ggaaaacggc gaagcctttc ctggagtcgt
    15421 ggattaaaga tcaggtcggt attcctgcgc tggtgagagc atttaaagaa aaagcgccgt
    15481 tctgggtcga aaaaatgcca gaactgcctg aactggttta cgacagtttg cgccagggca
    15541 agtatttaca gcacagtgtt gataagattg cccgcgagct tcagtcaaat catgtacgtc
    15601 agggacaatc gcgttatttt ctcggaattg gcgctacgtt agtattaagt ggcacattct
    15661 tgttggtcag ccgacctgaa tgggggctga tgcccgtctg gttaatggca ggtggtctga
    15721 tcgcctggtt tgtcggttgg cgcaaaacac gctgattttt tcatcgctca aggcgggctg
    15781 tgtaacgtat aatgcggcct tgtttaatca tcatctacca cagaggaaca tgtatgggtg
    15841 gtatcagtat ttggcagtta ttgattattg ccgtcatcgt tgtactgctt tttggcacca
    15901 aaaagctcgg ctccatcggt tccgatcttg gtgcgtcgat caaaggcttt aaaaaagcaa
    15961 tgagcgatga tgaaccaaag caggataaaa ccagtcagga tgctgatttt actgcgaaaa
    16021 ctatcgccga taagcaggcg gatacgaatc aggaacaggc taaaacagaa gacgcgaagc
    16081 gccacgataa agagcaggtg taatccgtgt ttgatatcgg ttttagcgaa ctgctattgg
    16141 tattcatcat cggcctcgtc gttctggggc cgcaacgact gcctgtggcg gtaaaaacgg
    16201 tcgcgggctg gattcgcgcg ttgcgttcac tggcgacaac ggtgcagaac gaactgaccc
    16261 aggagttaaa actccaggag tttcaggaca gtctgaaaaa ggttgaaaag gcgagcctca
    16321 ctaacctgac acccgaactg aaagcgtcga tggatgaatt acgccaggcc gcggagtcga
    16381 tgaagcgttc ctacgttgca aacgatcctg aaaaggcgag cgatgaagcg cacaccatcc
    16441 ataacccggt ggtgaaagac aatgaagctg cgcatgaagg cgtaacgcct gccgctgcac
    16501 aaacgcaggc cagttcgccg gaacagaagc cagaaaccac gccagagccg gtggtaaaac
    16561 ctgctgcgga cgctgaaccg aaaaccgctg caccttcccc ttcgtcgagt gataaaccgt
    16621 aaacatgtct gtagaagata ctcaaccgct tatcacgcat ctgattgagc tgcgtaagcg
    16681 tctgctgaac tgcattatcg cggtgatcgt gatattcctg tgtctggtct atttcgccaa
    16741 tgacatctat cacctggtat ccgcgccatt gatcaagcag ttgccgcaag gttcaacgat
    16801 gatcgccacc gacgtggcct cgccgttctt tacgccgatc aagctgacct ttatggtgtc
    16861 gctgattctg tcagcgccgg tgattctcta tcaggtgtgg gcgtttatcg ccccagcgct
    16921 gtataagcat gaacgtcgcc tggtggtgcc gctgctggtt tccagctctc tgctttttta
    16981 tatcggcatg gcgttcgcct actttgtggt ctttccgctg gcatttggct tccttgccaa
    17041 taccgcgccg gaaggggtac aggtatccac cgacatcgcc agctatttaa gcttcgttat
    17101 ggcgttgttt atggcgtttg gtgtctcctt tgaagtgcct gtggcaattg tgctgctgtg
    17161 ctggatgggg attacctcgc cagaagactt acgcaaaaaa cgcccgtatg tgctggttgg
    17221 tgcattcgtt gtcgggatgt tgctgacgcc gccggatgtc ttctcgcaaa cgctgctggc
    17281 gatcccgatg tactgcctgt ttgaaatcgg tgtcttcttc tctcgctttt acgttggtaa
    17341 gggtcgaaac cgggaagagg aaaacgacgc tgaagcagaa agcgaaaaaa ctgaagaata
    17401 aattcaaccg cccgtcaggg cggttgtcat atggagtaca ggatgtttga tatcggcgtt
    17461 aatttgacca gttcgcaatt tgcgaaagac cgtgatgatg ttgtagcgcg cgcttttgac
    17521 gcgggagtta atgggctact catcaccggc actaacctgc gtgaaagcca gcatgcgcaa
    17581 aagctggcgc gtcagtattc gtcctgttgg tcaacggcgg gcgtacatcc tcacgacagc
    17641 agccagtggc aagctgcgac tgaagaagcg attattgagc tggccgcgca gccagaagtg
    17701 gtggcgattg gtgaatgtgg tctcgacttt aaccgcaact tttcgacgcc ggaagagcag
    17761 gaacgcgctt ttgttgccca gctacgcatt gccgcagaat taaacatgcc ggtatttatg
    17821 cactgtcgcg atgcccacga gcggtttatg acattgctgg agccgtggct ggataaactg
    17881 cctggtgcgg ttcttcattg ctttaccggc acacgcgaag agatgcaggc gtgcgtggcg
    17941 cgtggaattt atatcggcat taccggttgg gtttgcgatg aacgacgcgg gctggagctg
    18001 cggaaattgt tgccgttgat tccggcggag aaattgctga tcgaaactga tgcgccgtat
    18061 ctgctcccgc gcgatctcac gccgaaacca tcatcccggc gcaacgagcc agcccatctg
    18121 ccccatattt tgcaacgtat tgcgcactgg cgtggagaag atgccgcatg gctggctgcc
    18181 accacggatg ccaatgttaa aacactgttt gggattgcgt tttagagttt gcggaactcg
    18241 gtgttcttca cactgtgctt aatctcttta ttgataagat taagtaacag catggagcga
    18301 gcctcgccgt cgggttcggt gaaaatggcc tgaaaacctt cgaacgcgcc ttcggtaata
    18361 atcaccttat cacccggata aggggttgcc ggatcgacaa tgtctttcgg tttatatacc
    18421 gatagctgat gaatcaccgc cgatgggact atcgctggcg acgcgccaaa gcgcacgaag
    18481 tggctgacac cgcgggtcgc gttgatagtc gtggtatgaa tcacttccgg atc
//
LOCUS       DAMZFT010000093        18476 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.093, whole genome shotgun sequence.
ACCESSION   DAMZFT010000093 DAMZFT010000000
VERSION     DAMZFT010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18476)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18476)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18476
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.093"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..771
                     /locus_tag="PN977_003402"
     CDS             <1..771
                     /locus_tag="PN977_003402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HDI6043406.1"
                     /translation="HTKHLMNPEVLAAARRIVHQVVEEIMARLAKEVRQAFSGVRDRR
                     RRSFISLVRNFDFKSTLRANLQHWHPQHGKLYIESPRFNSRIKRQSEQWQLVLLVDQS
                     GSMVDSVIHSAVMAACLWQLPGIRTHLVAFDTSVVDLTADVADPVELLMKVQLGGGTN
                     IASAVEYGRQLIEQPAKSVIILVSDFYEGGSSSLLTHQVKKCVQSGVKVLGLAALDST
                     ATPCYDRDMAQALVNVGAQIAAMTPGELAAWLAENLQS"
     gene            768..2768
                     /locus_tag="PN977_003403"
     CDS             768..2768
                     /locus_tag="PN977_003403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HDI6043407.1"
                     /translation="MNSLRPELLELTPQALTALSNAGFVKRSLKELENGNVPEISHKN
                     GALIATFSDGVRTQLANGQALKEAQCSCGASGMCRHRVMLVLSYQRLCATTQPTEKEE
                     EWDPAIWLEELATLPDATRKRAQALVAKGITIELFCTPGEIPSARLPMSDVRFYSRSS
                     IRFARCDCIEGTLCEHVVLAVQAFVQAKAQQAEFNHLIWQMRSEHVTSSDDPFASEEG
                     NACRQYVQQLSQALWLSGISQPLIHYEAAFSRAQQAAERCNWRWVSESLRQLRASVDA
                     FHARASHYHAGECLRQLAALNSRLNCAQEMARRDSVGEVPPVPWRTVVGAGIAGEAKL
                     DHLRLVSLGMRCWQDIEQYGLRIWFTDPDTGSILHLSRSWPRSEQENSPAATRRLFSF
                     LAGALAGGQIVSQAAKRSADGELLLATRNRLSSVVPLSPDAWQMLSAPLRQPGIVALR
                     EYLRQRPPACIRPLNQVDNLFILPVAECISLGWDSSRQTLDAQVISGEGEDNVLTLSL
                     PASACSPFAVERMAALLQQTDDPVSLVSGFVSFVEGQLTLEPRVMMTKTRAWALDAET
                     APVAPLPSASVLPVPSTAHQLLMRCQALLIQLLHNGWRYQEQSAIGQAELLANDLTAV
                     GFYRLAHVLGQFRNTESEARVEAMNNGVLLCEQLFPMLQQQG"
     gene            2893..3354
                     /locus_tag="PN977_003404"
     CDS             2893..3354
                     /locus_tag="PN977_003404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehR family lipoprotein"
                     /protein_id="HDI6043408.1"
                     /translation="MKAFNKLFSLVVASVLVFSLAGCGDKEESKKFSANLNGTEIAIT
                     YVYKGDKVLKQSSETKIQFASIGATTKEDAARTLEPLSAKYKNIAGVEEKLTYTDTYA
                     QENVTIDMEKVDFKALQGISGINVSAEDAKKGITMAQMELVMKAAGFKEVK"
     gene            complement(3395..3865)
                     /locus_tag="PN977_003405"
     CDS             complement(3395..3865)
                     /locus_tag="PN977_003405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HDI6043409.1"
                     /translation="MLSNDILRSVRYILKANNNDLVRILALGNVEATAEQIAVWLRKE
                     DEEGFQSCPDIVLSSFLNGLIYEKRGKDESAPALEPERRINNNIVLKKLRIAFSLKTD
                     DILAILTEQQFRVSMPEITAMMRAPDHKNFRECGDQFLRYFLRGLAARQHVKKS"
     gene            complement(3912..4631)
                     /gene="yehT"
                     /locus_tag="PN977_003406"
     CDS             complement(3912..4631)
                     /gene="yehT"
                     /locus_tag="PN977_003406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310963.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BtsR"
                     /protein_id="HDI6043410.1"
                     /translation="MIKVLIVDDEPLARENLRVFLQEQSDIEIVGECSNAVEGIGAVH
                     KLRPDVLFLDIQMPRISGLEMVGMLDPEHRPYIVFLTAFDEYAIKAFEEHAFDYLLKP
                     IDEVRLEKTLARLRQERSKQDVSLLPENQQALKFIPCTGHSRIYLLQMKDVAFVSSRM
                     SGVYVTSHEGKEGFTELTLRTLESRTPLLRCHRQYLVNLAHLQEIRLEDNGQAELILR
                     NGLTVPVSRRYLKSLKEAIGL"
     gene            complement(4628..6313)
                     /gene="btsS"
                     /locus_tag="PN977_003407"
     CDS             complement(4628..6313)
                     /gene="btsS"
                     /locus_tag="PN977_003407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulatory system sensor histidine
                     kinase BtsS"
                     /protein_id="HDI6043411.1"
                     /translation="MYDFNLVLLLLQQMCVFLVIAWLMSKTPLFIPLMQVTVRLPHKF
                     LCYIVFSIFCIMGTWFGLHIDDSIANTRAIGAVMGGLLGGPVVGGLVGLTGGLHRYSM
                     GGMTALSCMISTIVEGLLGGLVHSILIRRGRTDKVFNPITAGAVTFVAEMVQMLIILA
                     IARPYEDAVRLVSNIAAPMMVTNTVGAALFMRILLDKRAMFEKYTSAFSATALKVAAS
                     TEGILRQGFNEENSMKVAQVLYQELDIGAVAITDREKLLAFTGIGDDHHLPGKPISST
                     YTLKAIETGEVVYADGNEVPYRCSLHPQCKLGSTLVIPLRGENQRVMGTIKLYEAKNR
                     LFSSINRTLGEGIAQLLSAQILAGQYERQKAMLTQSEIKLLHAQVNPHFLFNALNTIK
                     AVIRRDSEQASQLVQYLSTFFRKNLKRPSEFVTLADEIEHVNAYLQIEKARFQSRLQV
                     NIAIPQELSQQQLPAFTLQPIVENAIKHGTSQLLDTGRVAISARREGQHLMLEIEDNA
                     GLYQPVTNASGLGMNLVDKRLRERFGDDYGISVACEPDSYTRITLRLPWRDEA"
     gene            6535..7266
                     /gene="mlrA"
                     /locus_tag="PN977_003408"
     CDS             6535..7266
                     /gene="mlrA"
                     /locus_tag="PN977_003408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MlrA"
                     /protein_id="HDI6043412.1"
                     /translation="MALYTIGEVALLCDINPVTLRAWQRRYGLLKPQRTDGGHRLFND
                     ADIDRIREIKRWIDNGVQVSKVKMLLSNENVDVQNGWRDQQETLLTYLQSGNLHSLRT
                     WIKERGQDYPAQTLTTHLFIPLRRRLQCQQPTLQALLAILDGVLINYIAICLASARKK
                     QGKDALVVGWNIHDTTRLWLEGWIASQQGWRIDVLAHSLNQLRPELFEGRTLLVWCGE
                     NRTSAQQQQLTSWQEQGHDIFPLGI"
     gene            7326..7433
                     /locus_tag="PN977_003409"
     CDS             7326..7433
                     /locus_tag="PN977_003409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YohO"
                     /protein_id="HDI6043413.1"
                     /translation="MRIAKIGVIALFLFMALGGIGGVMLAGYTFILRAG"
     gene            complement(7414..8145)
                     /gene="yehW"
                     /locus_tag="PN977_003410"
     CDS             complement(7414..8145)
                     /gene="yehW"
                     /locus_tag="PN977_003410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehW"
                     /protein_id="HDI6043414.1"
                     /translation="MKMLRDPLFWLIALFVALIFWLPYSQPLFAALFPQLPRPVYQQE
                     SFAALALAHFWLVGISSLFAVIIGTGAGIIVTRPWGAEFRPLVETIAAVGQTFPPVAV
                     LAIAVPVIGFGLQPAIIALILYGVLPVLQATLAGLGAIDASVTEVAKGMGMSRGQRLR
                     KVELPLAAPVILAGVRTSVIINIGTATIASTVGASTLGMPIIIGLSGFNTAYVIQGAL
                     LVALAAIIADRLFERLVQAFSQHAK"
     gene            complement(8150..9066)
                     /gene="yehX"
                     /locus_tag="PN977_003411"
                     /pseudo
     CDS             complement(8150..9066)
                     /gene="yehX"
                     /locus_tag="PN977_003411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708030.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter ATP binding
                     protein YehX"
     gene            complement(9059..10216)
                     /gene="yehY"
                     /locus_tag="PN977_003412"
     CDS             complement(9059..10216)
                     /gene="yehY"
                     /locus_tag="PN977_003412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehY"
                     /protein_id="HDI6043415.1"
                     /translation="MTYLRINPVLALLLLLTAIAAALPFISYAPNRLVSGEGRHLWQL
                     WPQTIWMLVGVCCAWLTACFIPGRKGSIFALILAQFVFVLLVWGAGKAATQLAQNGSA
                     LARTSLGSGFWLAAALALLACSDAIRRISTHPLWRWLLHMQIAIIPLWLLYSGALNDL
                     SLMKEYANRQDVFDDALAQHLTLLFGAVLPALVIGVPLGIWCYFSTARQGAIFSLLNV
                     IQTVPSVALFGLLIAPLAALVTAFPWLGKLGIAGTGMTPALIALVLYALLPLVRGVVV
                     GLNQIPRDVLESARAMGMSGAQRFLHVQLPLALPVFLRSLRVVMVQTVGMAVIAALIG
                     AGGFGALVFQGLLSSAIDLVLLGVIPVIVLAVLTDALFDLLIALLKVKRND"
     gene            complement(10223..11140)
                     /gene="osmF"
                     /locus_tag="PN977_003413"
     CDS             complement(10223..11140)
                     /gene="osmF"
                     /locus_tag="PN977_003413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein OsmF"
                     /protein_id="HDI6043416.1"
                     /translation="MPLSKVWAGSLVLLAAVSLPLHAASPVKVGSKIDTEGALLGNII
                     LQVLESHGVPTVNKVQLGTTPVVRGAITSGELDIYPEYTGNGAFFFKDENDTAWKNAG
                     QGYEKVKKLDAEQNKLIWLTPAPANNTWTIAVRQDVAEKNKLTSLADLSRYLQEGGTF
                     KLAASAEFIERADALPAFEKAYGFKLGQDQLLSLAGGDTAVTIKAAAQQTSGVNAAMA
                     YGTDGPVAALGLQTLSDPQGVQPIYAPAPVVRESVLKEYPQMAQWLQPVFASLDEKTL
                     QQLNASIAVEGLDAKKVAADYLKQKGWTK"
     gene            complement(11392..13689)
                     /gene="bglX"
                     /locus_tag="PN977_003414"
     CDS             complement(11392..13689)
                     /gene="bglX"
                     /locus_tag="PN977_003414"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucosidase BglX"
                     /protein_id="HDI6043417.1"
                     /translation="MKWLCSVGIAVSLALQPALADDLFGNHPLTPEARDAFVTELLKK
                     MTVDEKIGQLRLISVGPDNPKEAIREMIKDGQVGAIFNTVTRQDIRAMQDQVMELSRL
                     KIPLFFAYDVLHGQRTVFPISLGLASSFNLDAVKTVGRVSAYEAADDGLNMTWAPMVD
                     VSRDPRWGRASEGFGEDTYLTSIMGKTMVEAMQGKSPADRYSVMTSVKHFAAYGAVEG
                     GKEYNTVDMSPQRLFNDYMPPYKAGLDAGSGAVMVALNSLNGTPATSDSWLLKDVLRD
                     QWGFKGITVSDHGAIKELIKHGTAADPEDAVRVALKSGINMSMSDEYYSKYLPGLIKS
                     GKVTMEELDDAARHVLNVKYDMGLFNDPYSHLGPKESDPVDTNAENRLHRKEAREVAR
                     ESLVLLKNRLETLPLKKSATIAVVGPLADSKRDVMGSWSAAGVADQSVTVLTGIKNAV
                     GENGKVLYAKGANVTSDKGIIDFLNQYEEAVKVDPRSPQEMIDEAVQTAKQSDVVVAV
                     VGEAQGMAHEASSRTDITIPQSQRDLIAALKATGKPLVLVLMNGRPLALVKEDQQADA
                     ILETWFAGTEGGNAIADVLFGDYNPSGKLPMSFPRSVGQIPVYYSHLNTGRPYNADKP
                     NKYTSRYFDEANGALYPFGYGLSYTTFTVSDVKLSAPTMKRDGKVTASVQVTNTGKRE
                     GATVVQMYLQDVTASMSRPVKQLKGFEKITLKPGETQTVSFPIDIEALKFWNQQMKYD
                     AEPGKFNVFIGTDSARVKKGEFELL"
     gene            13885..15600
                     /gene="dld"
                     /locus_tag="PN977_003415"
     CDS             13885..15600
                     /gene="dld"
                     /locus_tag="PN977_003415"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HDI6043418.1"
                     /translation="MSSMTTTDNKAFLNELARLVGHSHLLTDPAKTARYRKGFRSGQG
                     DALAVVFPGSLLELWRVLKACVTADKIILMQAANTGLTEGSTPNGNDYDRDIVIISTL
                     RLDKLHVLGKGEQVLAYPGTTLYSLEKALKPLGREPHSVIGSSCIGASVIGGICNNSG
                     GSLVQRGPAYTEMSLFARINEDGKLTLVNHLGIDLGETPEQILSKLDDDRIKDDDVRH
                     DGRHAHDYDYVHRVRDIEANTPARYNADPDRLFESSGCAGKLAVFAVRLDTFEAEKNQ
                     QVFYIGTNQPEVLTEIRRHILANFENLPVAGEYMHRDIYDIAEKYGKDTFLMIDKLGT
                     DKMPFFFNLKGRTDAMLEKVKFFRPHFTDRAMQKFGHLFPSHLPPRMKNWRDKYEHHL
                     LLKMAGDGVGEAKSWLVDYFKQAEGDFFVCTPEEGSKAFLHRFAAAGAAIRYQAVHSD
                     EVEDILALDIALRRNDTEWYEHLPPEIDNQLVHKLYYGHFMCYVFHQDYIVKKGVDVH
                     ALKEQMLELLQQRGAQYPAEHNVGHLYKAPETLQKFYRENDPTNSMNPGIGKTSKRKN
                     WQEVE"
     gene            complement(15639..16571)
                     /gene="pbpG"
                     /locus_tag="PN977_003416"
     CDS             complement(15639..16571)
                     /gene="pbpG"
                     /locus_tag="PN977_003416"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416638.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine endopeptidase"
                     /protein_id="HDI6043419.1"
                     /translation="MPKFRVSLFSLALMLAVPFAPQAVAKTAAATTASQPEIASGSAM
                     IVDLNTNKVIYSNHPDLVRPIASISKLMTAMVVLDARLPLDEKLKVDISQTPEMKGVY
                     SRVRLNSEISRKDMLLLALMSSENRAAASLAHHYPGGYKAFIKAMNAKAKSLGMNNTR
                     FVEPTGLSVHNVSTARDLTKLLIASKQYPLIGQLSTTREDMATFSNPTYTLPFRNTNH
                     LVYRDNWNIQLTKTGFTNAAGHCLVMRTVINNKPVALVVMDAFGKYTHFADASRLRTW
                     IETGKVMPVPAAALSYKKQKAAQMAAAGQTAQND"
     gene            complement(16745..17332)
                     /locus_tag="PN977_003417"
     CDS             complement(16745..17332)
                     /locus_tag="PN977_003417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014170891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YIP1 family protein"
                     /protein_id="HDI6043420.1"
                     /translation="MSHVWGLFSHPDREMQVINRENETISHHYTHHVLLMAAIPVICA
                     FIGTTQIGWNFGDGTILKLSWFTGLALAVLFYGVMLAGVAVMGRVIWWMARNYPQRPS
                     LAHCMVFAGYVATPLFLSGLVALYPLVWLCALVGTVALFYTGYLLYLGIPSFLNINKE
                     EGLSFSSSTLAIGVLVLEVLLALTVILWGYGYRLF"
     gene            17502..18080
                     /locus_tag="PN977_003418"
     CDS             17502..18080
                     /locus_tag="PN977_003418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HDI6043421.1"
                     /translation="MDLNTLISQYGYAALVIGSLAEGETVTLLGGVAAHQGLLKFPLV
                     VLSVALGGMIGDQVLYLCGRRFGGKLLRRFSKHQDKIERAQKLIQRHPYLFVIGTRFM
                     YGFRVIGPTLIGASQLPPKIFLPLNILGAFAWALIFTTIGYAGGQVIAPWLHNLDQHL
                     KHWVWLILVVVLVVGVRWWLKRRGKKKPDHQA"
     gene            complement(18200..>18476)
                     /locus_tag="PN977_003419"
     CDS             complement(18200..>18476)
                     /locus_tag="PN977_003419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDI6043422.1"
                     /translation="LTKTMALELVRHKILVNAVAPGAIATPMNGMDGGDVKPDAEPSI
                     PLRRFGTTHEIASLVVWLCSEGANYTTGQSLIVDGGFMLANPQFNPE"
BASE COUNT         4482 a         5056 c         4679 g         4259 t
ORIGIN      
        1 cacaccaaac atctgatgaa tcccgaagtg ctggctgccg cccgccggat agtgcaccag
       61 gttgttgaag aaattatggc tcgactggca aaggaagttc gtcaggcttt ttctggagta
      121 cgcgatcgcc gtcgccgctc atttatttca ctggtgcgaa actttgattt caaaagtact
      181 ctgcgcgcca acctgcaaca ctggcacccg caacacggca agttgtatat cgaatccccc
      241 cgctttaaca gccgcattaa acgccaaagc gaacaatggc aactggtctt actggttgat
      301 caaagcggat cgatggttga ctcggtgatc cactctgcgg tgatggcggc ctgtttatgg
      361 cagttacccg gcattcgtac ccatctggtg gcgtttgaca ccagcgtcgt tgatctcacg
      421 gcagacgttg ctgatccggt agagttatta atgaaagtac aactgggcgg cgggaccaat
      481 atcgccagtg ccgtggagta tggtcggcaa cttattgaac aaccagcaaa aagcgtcatt
      541 atcctcgtga gcgatttcta tgaagggggt tcatcatcat tgctgacgca tcaggtgaaa
      601 aagtgtgtcc agagcggcgt caaagtgctg ggactggcgg cgctcgatag caccgcaaca
      661 ccttgctatg accgcgatat ggcccaggcg ctggttaatg tcggcgcaca aatagctgcc
      721 atgacaccgg gcgaactggc ggcctggctt gcggagaatc ttcagtcatg aattcactac
      781 gtccggaatt attagaactg acgccgcagg ccctgacggc gttaagcaat gccggttttg
      841 ttaaacgcag tcttaaggaa ctggaaaatg gcaacgtccc ggaaatcagc cataagaacg
      901 gcgctttaat cgccaccttc agtgacggtg tccgtaccca gctggcgaac ggtcaggcac
      961 tgaaagaggc tcaatgcagc tgcggggcca gcggcatgtg ccgccatcgc gtgatgctgg
     1021 ttttaagtta tcagcgactt tgcgccacca cccagcctac ggaaaaagaa gaagagtggg
     1081 atccggcaat ctggctagaa gaactggcta cccttcccga tgccacccgc aaacgcgcgc
     1141 aggcgctggt cgctaaaggc atcaccattg agttgttctg tacgccgggc gaaattccct
     1201 ctgcccgctt accgatgagc gatgtacgtt tttattcccg cagcagcatt cgtttcgcac
     1261 gctgtgattg tattgaagga acactttgcg aacatgttgt gctggcagta caggcctttg
     1321 tccaggccaa agcgcagcaa gcagaattta accatttaat ctggcagatg cgcagcgaac
     1381 acgtcacatc atctgacgat ccgtttgcca gcgaagaggg caacgcgtgt cgtcaatatg
     1441 ttcagcaact aagccaggca ttatggctga gcggcatcag ccagccgctt attcattacg
     1501 aggccgcttt cagccgcgcg cagcaagcgg cggaacgctg caactggcga tgggtgagtg
     1561 aatcgctacg gcaattgcgc gccagcgttg atgccttcca cgcccgcgcc agccactacc
     1621 atgccggaga atgcttacgt cagcttgcgg cattaaacag tcgattaaat tgcgcacaag
     1681 agatggcgcg gcgcgacagt gttggtgaag ttcctcctgt gccgtggcgc acggtcgttg
     1741 gcgctggcat tgccggagaa gcaaagcttg atcatctgcg gctggtgtct ttaggtatgc
     1801 gttgctggca ggatattgag cagtatggtt tacgcatctg gttcaccgat cccgacaccg
     1861 gcagtatttt gcatctttcg cgtagttggc cgagaagcga acaggaaaat tcaccggcag
     1921 cgacgcgtcg gctgtttagt tttctggctg gcgcactggc aggtgggcaa attgtttcac
     1981 aagcagcaaa acgcagtgcc gatggcgaac tgctattagc tacccgcaac cgcttgagta
     2041 gtgttgtgcc gctgtcgcca gacgcctggc aaatgttgag cgcgccattg cgccagccgg
     2101 gcattgtggc tttgcgtgaa tatttacgcc agcgtccccc cgcctgcata cgaccactca
     2161 accaggtcga taacttattt attctgccag tcgctgagtg tatttcactc ggttgggata
     2221 gcagccgcca gacactggat gcgcaggtaa tcagcggcga aggtgaagat aacgtgctga
     2281 cattatccct accagcctct gcctgttcgc cctttgctgt tgaacgcatg gcggcgcttt
     2341 tgcaacaaac agacgacccc gtgagtctgg tttctggctt tgtcagcttt gtagaggggc
     2401 aattgacact ggaaccacgg gtgatgatga caaaaacccg cgcctgggcg ctggacgcag
     2461 aaactgcgcc tgtagcaccg ctaccttctg ccagcgtttt gcctgtgccg tctaccgctc
     2521 atcagttgct gatgcgctgc caggcgttac ttattcaact gctccataac ggctggcgct
     2581 atcaggaaca gagtgctatt ggtcaggcag agttgctggc gaatgacctc accgcggtgg
     2641 gtttttatcg gctggcacat gtgttgggac aatttcgtaa tacagaaagc gaggcacggg
     2701 tagaagcaat gaataacggt gttttgcttt gcgaacaatt attccccatg cttcagcaac
     2761 aaggatgaaa tagtgctttt tattaagagt tctactcctg ttccggactg ctcacgccac
     2821 agtattaggc atatcctata tagcccctgg tgagagtcac cagttccttg attaaataaa
     2881 atggagtttt acatgaaggc tttcaataag ctgttttccc ttgttgttgc atctgttctg
     2941 gttttctctc ttgctggctg cggtgacaaa gaagaatcaa agaaattcag cgccaatctg
     3001 aacggcactg aaattgccat tacctatgtc tacaaaggtg acaaggtgct taagcaatct
     3061 tctgaaacca aaattcaatt tgcctccatt ggtgcaacca ccaaagaaga tgccgccagg
     3121 acacttgagc cgttaagcgc caaatacaaa aacatcgcgg gtgttgaaga aaaattaacc
     3181 tataccgata cctacgcgca ggaaaacgtg actattgata tggaaaaagt ggattttaaa
     3241 gccctgcagg gtatttcagg aatcaacgtt tctgctgaag atgccaaaaa aggtatcact
     3301 atggcgcaaa tggaactggt gatgaaagcc gctggtttta aagaagtgaa ataatctgtc
     3361 ggcggccatg tttcgcatgg ccgccatacc cgtcttagct tttcttcaca tgctggcgcg
     3421 ctgccagtcc acgcagaaaa taacgtaaaa attgatcgcc gcattcgcgg aagtttttat
     3481 gatccggtgc acgcatcatc gccgtaattt ccggcatcga aacgcggaac tgctgttcgg
     3541 tgaggattgc cagaatgtca tcggttttaa gcgaaaacgc gatgcgtaat tttttcagca
     3601 cgatgttgtt attaatgcga cgttccggct ccagtgccgg agcagactca tccttgccgc
     3661 gtttttcata aatcaggcca ttgaggaatg acgacaaaac aatgtccgga caactctgaa
     3721 aaccctcttc gtcttcttta cgtagccaga cggcgatctg ttccgcggtg gcttcgacat
     3781 tacccagcgc cagaatacgc accaggtcat tattattggc tttcaaaatg tagcgcacgc
     3841 tgcgcagaat atcgttactt agcatgaggc cttcaggtgt tgatgaggca aaaagccatt
     3901 ttagcagtct tttacaggcc aatcgcctct tttaagcttt tcagataacg gcggctgacc
     3961 ggcacggtta agccattacg caaaatcaac tcggcctggc cgttatcttc cagacgaatc
     4021 tcctgtaaat gcgcgaggtt aaccagatac tgacgatggc agcgcagtaa tggtgtacga
     4081 ctttccaggg tacgtaatgt cagttcggta aagccctctt tcccttcgtg actggtaacg
     4141 tagacaccgc tcatccgact gctgacaaat gccacatctt tcatttgcag caaataaatc
     4201 cgactatgcc ccgtacaagg gataaatttc agcgcctgtt gattttccgg taacagcgaa
     4261 acatcctgct tgctgcgctc ctgacgcaat cgcgccagcg ttttctccag tcgcacttca
     4321 tcaattggct tcagcagata atcaaaggca tgttcttcaa aggctttaat ggcgtattcg
     4381 tcaaacgcgg tgagaaaaac aatatacggg cgatgttccg ggtcgagcat ccccaccatt
     4441 tccagaccac tgatgcgcgg catctggata tcgagaaaca gcacatccgg gcgcagttta
     4501 tgcaccgcgc cgatcccttc tacggcgttt gaacactctc caacgatttc aatatcgctc
     4561 tgctcctgca aaaatacgcg caggttctcc cgtgctaacg gttcatcatc gacaattaag
     4621 actttaatca tgcctcgtcc ctccatggta gtcgtaacgt tattcgggtg taactatcag
     4681 gctcacaggc gacgcttatc ccatagtcat cgccaaaccg ttcacgtaaa cgcttatcca
     4741 ccagattcat ccccagccca ctggcattgg ttaccggttg atacaaacct gcattgtctt
     4801 cgatctccag catcaaatgt tgcccctcac gtcgggcgct gattgccact cgccctgtat
     4861 ccagcagttg tgatgtccca tgtttaatgg cgttttccac tatcggttgc agggtaaacg
     4921 cgggcaattg ctgctgggat aattcttgcg gaatagcaat gttgacctgc aaccgcgact
     4981 ggaagcgcgc cttttcaatt tgcagataag cgttcacatg ttcaatttcg tcggcgagag
     5041 taacaaactc cgaaggccgc tttaagtttt tgcggaaaaa agtggaaaga tactgcacca
     5101 gctggctggc ctgttcgctg tcgcggcgga tcaccgcttt aatggtgtta agcgcattaa
     5161 acaaaaaatg gggattcacc tgggcgtgaa gcagtttgat ctctgactgg gtgagcatcg
     5221 ctttttgccg ctcatattgc ccggcgagga tctgcgccga aagcagttgc gcaatcccct
     5281 cgcccagcgt gcggttgatt gaactgaata aacggttttt ggcttcatac aatttgatgg
     5341 tacccatcac tcgctgattt tcaccacgca acggaatgac cagcgtcgac cccagtttgc
     5401 attgcggatg caaagagcaa cggtaaggca cttcgttgcc atcagcgtag accacttcac
     5461 cggtttcaat cgcttttaag gtataagttg aagaaatcgg tttgccgggt aaatggtggt
     5521 cgtcaccaat tccggtaaag gccagcaatt tctctcgatc ggtaatcgcg actgcaccaa
     5581 tatccagctc ctgatacagc acctgtgcca ctttcatgct gttctcttcg ttaaacccct
     5641 ggcgcaaaat gccttccgtc gaggccgcca ctttcagcgc agtggcagaa aaagccgaag
     5701 tgtatttttc aaacatcgcg cgtttatcga gcaatatacg cataaacagt gccgcgccga
     5761 cggtattggt gaccatcatt ggcgcagcaa tattactcac cagacgcacc gcatcttcat
     5821 aaggtcgggc gatcgccagg atgatcaaca tttgcaccat ttcagcgacg aacgtgacgg
     5881 caccggcggt aatggggtta aagactttat cagtgcgccc gcgacggatc aggatgctgt
     5941 gtaccaggcc gccgagcaat ccttcaacga tggtcgagat catgcaactt agcgcggtca
     6001 tgccccccat cgaatatcga tgtaacccgc cggtcagacc aaccagccca ccgacgaccg
     6061 gaccgccgag taagccgccc attaccgcgc ctatcgcacg ggtattggca atagaatcgt
     6121 caatgtgcaa cccaaaccag gtgcccatga tgcagaagat ggaaaagacg atgtagcaga
     6181 gaaatttatg cggcagacga accgtgacct gcattagcgg tatgaataat ggcgttttac
     6241 tcattaacca cgcaatgact aaaaaaacgc acatctgctg aagcagcagc aacaccagat
     6301 taaaatcgta catacccgca aaccacactt ccctttaaaa cgcgtaacat acattgcttg
     6361 cgtttaactt tctttgaact cttgcagaaa aatgagaatt cgtgagtacg atcactcaaa
     6421 atcgcctggc aaaaataaaa tcaccctata gatgcacaaa aaacgggcaa aactacctgg
     6481 ttcgcaaaac tgcgtctaaa gttaaaccgg gacctcgcga gcaagggtga gacgatggcg
     6541 ctttacacaa ttggtgaagt ggcgttgctt tgtgatatta accctgtcac gttacgcgcg
     6601 tggcagaggc gttacggatt gctgaaaccg caacggacag acggcggtca tcggctgttc
     6661 aacgatgccg atattgaccg gatccgcgag atcaaacgct ggatcgacaa cggcgtgcag
     6721 gtcagcaaag taaaaatgct gctcagtaat gaaaatgttg atgtgcagaa cggctggcgc
     6781 gatcagcaag aaacattact gacttacctg caaagcggca atctgcatag cctgcgaacg
     6841 tggatcaaag agcgcggtca ggattacccc gcccagacac tcaccacaca tctgtttatt
     6901 cctctgcgcc gacgacttca gtgtcaacaa ccgactctcc aggcgctgct agcgatcctc
     6961 gacggcgtac tgatcaacta catcgccatc tgtctggctt cggcacgtaa aaaacagggt
     7021 aaagatgcgc tggtggttgg ctggaatatt cacgatacca cccgtctgtg gctggagggc
     7081 tggattgcca gtcaacaagg atggcgcatt gatgtcctcg cccactcgct caatcaacta
     7141 cgccctgaac tgttcgaagg ccgtacattg ctggtgtggt gcggtgaaaa tcgaacctcc
     7201 gcccaacagc agcaactcac cagttggcag gaacaaggcc atgatatttt cccactcggc
     7261 atttaatgat tcgttaacaa atgcgcttta ctgtacaatc ctttcgttaa cataaggagt
     7321 gcattatgcg catagctaaa attggggtca tcgccctgtt cctgtttatg gcgttaggcg
     7381 gaattggtgg cgtcatgctc gcaggttata cctttatttt gcgtgctggc tgaacgcctg
     7441 caccagcctt tcaaacaggc ggtctgcgat gatcgccgcc agtgccacca gtaacgcccc
     7501 ctggatcaca tacgcggtat taaatccgct aagcccgatg atgatcggca tacccagcgt
     7561 gctggcccct accgttgagg cgatcgtcgc cgtaccaatg ttgataatca ccgaagttcg
     7621 cacgcccgcc agaatcaccg gagccgccag cggtagctcg accttacgca gtcgctgacc
     7681 acgactcatt cccatacctt tcgcaacttc tgtcacgctg gcatcaatcg ctcccagccc
     7741 ggcaagtgtc gcctgcagga cgggcagcac gccgtaaagg atcaaggcga taatcgcggg
     7801 ttgcagacca aagccgatca ccggaacggc aatcgccagc accgcgacgg gcggaaaagt
     7861 ctgcccaacg gcggcaatag tttccaccag tgggcgaaat tccgcgcccc acgggcgagt
     7921 aacaataatt cctgcaccag tgccaatgat caccgcaaac aaactcgaaa ttcccaccag
     7981 ccagaaatga gccagtgcca gagctgcaaa actttcttgc tgataaacgg ggcgtggcag
     8041 ttgtgggaac aaggcagcaa acagcggctg gctgtaaggc agccagaaaa tcagtgccac
     8101 aaacaaagca atgagccaga acagcggatc gcgcaacatc ttcatacgct tacgcatcca
     8161 ccagcagatc ctgaaaatgc agcgtgccgc aaggctcgcc ctgtgtgttc accaccggca
     8221 gcacttcaca tccccgcgcg acaacgcatc gcgtagcgtc atctcttctg ccagcgcctc
     8281 accttctgcc cgttcttcgc gacgcacgta atccgccaca ctacgtaacg aaagcaggcg
     8341 cacacccagt tcactacgtc caaaaaactg gcgaacaaaa tcattcgccg gacgagtcag
     8401 catcgtcagc ggattgccct gctgcaccac ttcaccgtga tccatcaata ccagatgttc
     8461 tgccagccgt agcgcctcat caatatcatg agtgaccagc acaatagtgc gccccagcaa
     8521 acggtgaatg cgcgtcatct cttgttgcaa cgcgccgcgc gttaccgggt ccagtgcgcc
     8581 aaaaggttca tccatcagta agacttgcgg atcggcagcc agtgcgcgcg ccactcccac
     8641 acgttgctgc tgaccaccgg aaagctgatg cggataacgc tcacgtaaat ttgactccag
     8701 ccccagtagc gccattaatt cgtcgatacg atcgtcaatc cgcgcccgcg accatttttg
     8761 taattgcggc acggtagcga tgttttgcgc cacgctccag tgggggaaca ggccaataga
     8821 ttgaatggca tagcccatcc ggcggcgcaa ctccagtact ggcagcgagc gaatttcttc
     8881 tccggcaaag cggatcacgc cgctgtcatg ctccaccagg cggttaatca ttttcagggt
     8941 ggtggatttg ccggagccag atgtgccaat cagcaccgaa aaactccctt cctgaaaatt
     9001 gagattgaga tcgttaacgg ctttttgtgc gccgaacagt ttgctgacat ggctaaattc
     9061 aatcattacg tttcaccttc agcagtgcga taagcaaatc gaacagcgcg tcggtaagca
     9121 ccgccagaac aattaccggg atcaccccca gcaacactaa atcaatggcg ctgcttagca
     9181 gcccctggaa aaccagcgca ccaaaaccgc ctgcgccgat taacgccgca atcaccgcca
     9241 tgcctacagt ttgcaccatc accacccgca ggctgcgcaa aaataccggt aacgccagcg
     9301 gtaactgaac atgcaggaat cgctgcgccc cgctcatccc catcgctctg gcgctctcca
     9361 gcacatcgcg cgggatctgg ttcaagccga ctaccacgcc gcgcaccagc ggcagcaagg
     9421 catagagcac cagcgcaatc agtgcgggtg tcattccggt tcctgctatg cccagcttac
     9481 ccagccacgg aaaggccgta accagcgcgg caagcggcgc aatcaacagg ccaaagagcg
     9541 ccaccgaagg cacggtctga atgacattga gtagcgaaaa aatcgccccc tgccgcgcgg
     9601 tggaaaagta gcaccagatg cccaacggca caccaatcac taacgcaggc agcaccgcac
     9661 caaacagcaa cgtcagatgt tgcgccagcg cgtcatcaaa cacatcctga cggttggcgt
     9721 attctttcat tagcgagaga tcgttaagcg cgccggaata cagcaaccac agcggaatga
     9781 tggcaatctg catatgcaac aaccagcgcc acagcggatg cgtggagatt cgtcggatgg
     9841 catcgctaca ggcaagcaat gccagcgccg cagccagcca gaaaccactg ccgaggctgg
     9901 tacgcgccag cgcactgcca ttttgcgcca gttgtgtcgc cgcctttcca gctccccaca
     9961 ccagcaatac gaagacgaat tgcgccagaa tgagtgcaaa aatgctgcct tttctaccgg
    10021 gaataaaaca ggctgtcagc caggcgcaac atacgcccac cagcatccag atcgtttgcg
    10081 gccacaactg ccagagatga cgcccctcac cagagactaa acgattaggc gcgtaactga
    10141 taaacggcag cgccgctgcg attgccgtca gcaacagcag cagcgccaga acaggattaa
    10201 tacgtaaata agtcacggga aattacttcg tccacccttt ttgtttcagg tagtcggcag
    10261 ccactttttt ggcatccagt ccttcaacgg caatgctggc attcagttgc tgtaatgttt
    10321 tttcatcgag gctggcgaag actggctgta gccactgtgc catttgcgga tactctttca
    10381 acaccgactc tcgcaccact ggtgcaggcg cgtagatagg ctgaacgcct tgcggatcgc
    10441 ttaaggtttg cagccccagc gccgcgaccg gaccgtcagt gccgtaagcc attgcggcat
    10501 taacgccgga tgtttgctgg gcagcggcct tgatcgtcac cgccgtgtcg ccaccagcca
    10561 gtgacagcaa ctgatcctga ccgagcttaa agccataggc tttttcaaag gcgggtaacg
    10621 catcggcgcg ttcgataaac tctgctgagg cagccagttt gaaggtgccg ccctcttgca
    10681 gataacgact caggtcagca agcgaagtga gtttgttttt ctctgccaca tcctgacgca
    10741 cggcgatggt ccaggtgtta tttgcaggcg caggcgtcag ccagattaac ttgttttgct
    10801 ctgcatcgag ctttttgact ttctcgtagc cttgcccggc atttttccac gccgtatcgt
    10861 tttcatcttt aaagaaaaac gcgccattgc cagtatattc cggatagata tccagctcac
    10921 cagaagtgat cgccccgcgc actacgggag tcgttcccaa ttgcacttta tttaccgtag
    10981 gaacgccgtg actttccagc acctgcaaaa tgatattgcc gagcagcgcg ccttcggtat
    11041 cgatttttga accgacttta acgggggaag ccgcgtgcag cggcaggctc acggctgcca
    11101 acaaaaccag tgaacctgcc cagacctttg agagtggcat gatgctttcc tcattctttt
    11161 actgttgttt tcagcgaatt aagagaaaag catagttgat aatgacaggg ttagcctgag
    11221 cggacggatt cagttgcaga atcagataaa tacttaagag gcatattcgg tccggcgttt
    11281 cctgccggat gcggcgcgag cgccttatcc ggcctacaaa gggcgaaaat tcagcaagtt
    11341 aagatatgaa catcaggcaa agggcgcaaa cgtcgcgccc ttgctaaagc attacagcaa
    11401 ctcaaactcg cctttcttaa cgcgtgcgga atcagtgccg ataaagacat tgaacttgcc
    11461 aggctcggcg tcatatttca tctgttgatt ccagaacttc agcgcctcga tatcaatcgg
    11521 gaagctgacg gtctgggttt cgcccggttt cagggtgatt ttctcaaagc ctttcagctg
    11581 ttttaccggg cgactcatgg aagccgtcac atcctgcagg tacatctgaa ctaccgtcgc
    11641 cccttcgcgc ttaccggtgt tcgtcacctg cacgctggcg gtcactttgc cgtcacgctt
    11701 catggtcggc gcagaaagtt tcacatcaga gacggtgaaa gtggtatagc tcagaccata
    11761 gccgaacgga taaagcgccc cgttagcttc atcaaaataa cgcgaagtgt atttgttcgg
    11821 cttgtcggca ttatacgggc gaccggtatt cagatgactg tagtacaccg ggatctgccc
    11881 gacagaacgc gggaaggaca tcggcagctt gccggacggg ttgtaatcgc caaacaatac
    11941 atcggcaatt gcattaccgc cttcagtccc cgcaaaccag gtttccagaa tcgcatccgc
    12001 ctgctgatct tctttcacca gcgccagcgg acgcccgttc atcagcacca gcaccagcgg
    12061 tttaccggtg gctttcagcg ccgcaatcaa gtcacgttgg ctttgcggaa tagtgatatc
    12121 ggtacggctg gaggcctcgt gcgccatccc ctgagcttca ccgaccacag ccaccaccac
    12181 atcagattgc ttcgcggttt gcaccgcttc atcaatcatc tcttgcggcg agcgcgggtc
    12241 gactttgacc gcctcttcat actgattcag gaaatcgata atgcctttgt cactggtgac
    12301 gttcgcccct ttggcgtaca gcactttacc gttttcaccg acggcgtttt taatccctgt
    12361 tagcacagta acagattgat cggcgacacc tgctgccgac cagcttccca tcacgtcacg
    12421 cttgctgtct gccagcgggc caaccaccgc aatggtggct gattttttca gcggtaacgt
    12481 ttcgagacgg tttttcagca acaccaggct ttcgcgtgcc acttcacgcg cttctttgcg
    12541 gtgcaggcgg ttttcggcat tggtatccac cgggtcagac tctttcggac cgagatggct
    12601 gtacgggtcg ttaaacaacc ccatatcata tttaacgttc agtacatgac gggcagcgtc
    12661 atccagctct tccatcgtca ctttgccgga tttgatcaac ccaggcagat acttcgagta
    12721 atactcgtcg ctcatactca tgttgatgcc ggatttcagc gccacgcgca ccgcatcttc
    12781 cgggtctgcc gccgtgccat gtttaatcag ctctttgatt gcgccgtgat cggaaacggt
    12841 gatgccttta aagccccact ggtcgcgcag aacatctttc agcagccagg agtcggaggt
    12901 ggctggcgta ccgttcagcg agttcagcgc caccatcacc gcgccgctgc ctgcgtccag
    12961 ccccgctttg tacggcggca tataatcatt aaacaggcgc tgcggactca tatcgacggt
    13021 gttgtactct ttaccgcctt ctaccgcgcc gtatgcggca aagtgtttga cgctggtcat
    13081 caccgagtag cgatctgccg ggcttttacc ctgcatcgct tccaccatgg ttttgcccat
    13141 tattgaggtg agatacgtat cttcgccaaa accttcggaa gcacgtcccc agcgcggatc
    13201 gcgcgagaca tcgaccatcg gtgcccaggt catattcagg ccatcatctg ccgcttcata
    13261 agcagagaca cgcccgactg ttttcaccgc atcgaggtta aaagacgagg ccagaccgag
    13321 gctaatcggg aacaccgtgc gctgaccgtg cagcacgtcg taagcaaaga aaagaggaat
    13381 tttcaggcgg cttaattcca tcacctgatc ctgcatggcg cggatatcct gacgggttac
    13441 ggtgttgaaa atcgccccaa cctgcccgtc tttgatcatc tcgcggatcg cctctttcgg
    13501 attatccggg ccgacgctga ttaaacgcag ctgaccaatt ttctcatcaa ctgtcatttt
    13561 cttaagcagt tcggtgacga acgcatcccg cgcttcgggc gttaatggat ggttgccgaa
    13621 taaatcatcc gccagtgctg gctgcagggc cagactcacc gcgattccta ctgaacatag
    13681 ccatttcata tggatttttc ttcctctgtt gcccgacgag gcagcgaaaa tggtgcaaaa
    13741 accgtagttt gccataagca tgatggagag agaaaaagaa tgctcagttt attgtctgaa
    13801 ttttcaaaat attcactcgc tgaattgtca tacaaggcgc tattctagtt tgtgatattt
    13861 tttcgccacc acaaggagtg gaaaatgtct tccatgacaa caactgataa taaagccttt
    13921 ttgaatgaac ttgcccgtct ggtcggtcat tcacacctgc tcaccgatcc cgcaaaaacg
    13981 gcccgctatc gcaagggctt ccgttctggt cagggcgacg cgctggctgt cgttttccct
    14041 ggctcactac tggaattgtg gcgggtgctg aaagcctgcg tcaccgccga caaaattatt
    14101 ctgatgcagg ccgccaatac aggtctgacc gaaggctcga cgccaaacgg taacgactat
    14161 gatcgcgata tcgtgatcat cagcaccctg cgtctcgata agttgcacgt tctcggcaag
    14221 ggcgaacaag tgctggccta tccgggcacc acgctttatt cgctggaaaa agcactcaaa
    14281 ccgctgggac gcgaaccgca ctcggtgatt ggatcatcgt gtataggcgc atcggtcatc
    14341 ggcggtattt gtaacaactc cggcggctcg ctggtgcagc gtggcccggc gtataccgaa
    14401 atgtcgttat tcgcgcgtat aaatgaagac ggcaaactga cgctggtgaa ccatctcggg
    14461 attgatctgg gcgaaacgcc ggagcagatc cttagcaagc tggatgacga tcgcatcaaa
    14521 gatgacgatg tgcgtcacga tggtcgtcac gcccacgatt atgactatgt ccaccgcgtt
    14581 cgtgatattg aagccaacac gcccgcacgt tataacgccg atccggatcg gttatttgaa
    14641 tcttctggtt gcgcaggtaa gctggccgtc tttgcggtac gtcttgatac cttcgaagcg
    14701 gaaaaaaatc agcaggtgtt ttatatcggc accaaccagc cggaagtgct gaccgaaatc
    14761 cgccgtcata ttctggctaa tttcgaaaat ctgccggttg ccggggaata tatgcaccgg
    14821 gatatctacg atattgcgga aaaatacggc aaagacacct tcctgatgat tgataagtta
    14881 ggcaccgaca agatgccatt cttctttaat ctcaaggggc gcaccgatgc gatgctggag
    14941 aaagtgaaat tcttccgtcc ccattttacc gaccgtgcga tgcaaaaatt cggtcacctg
    15001 ttccccagcc atttaccgcc gcgcatgaaa aactggcgcg ataaatacga acatcatctg
    15061 ctgttaaaaa tggcgggcga tggcgtcggt gaagccaaat cgtggctggt ggattatttc
    15121 aaacaggccg aaggcgattt ctttgtctgt acgccggagg aaggtagcaa agcgttttta
    15181 caccgtttcg ccgctgcggg cgcagcaatt cgttatcagg cggtgcattc cgatgaagtc
    15241 gaagacattc tggcactgga tatcgctctg cggcgtaacg acaccgagtg gtatgagcat
    15301 ttaccgccgg agatcgacaa ccaactggtg cacaagctct attacggcca ttttatgtgc
    15361 tatgtcttcc atcaggatta catcgttaaa aaaggcgtgg atgtgcatgc gttgaaagaa
    15421 cagatgctgg aactgctgca gcagcgtggc gcgcaatacc ctgccgagca taacgtcggt
    15481 catttgtata aagcaccgga gacgttgcag aagttctatc gcgagaacga tccgaccaac
    15541 agtatgaatc cggggatcgg taaaaccagt aagcggaaaa actggcagga agtggagtaa
    15601 aaattacgga tggcagagta tcgccatccg tacttcactt aatcgttctg cgccgtctgc
    15661 cccgccgccg ccatctgggc ggctttttgt tttttatagc tcaacgctgc tgccggaaca
    15721 ggcatcactt taccggtctc aatccaggta cgcaggcggc tggcatcggc aaaatgggta
    15781 tatttgccaa acgcgtccat tactaccagc gccaccggtt tattattgat aaccgtacgc
    15841 atcaccagac aatggcccgc cgcattggta aagccggttt tggttaactg aatattccag
    15901 ttatcgcgat acaccagatg gttagtattg cggaacggca gcgtatacgt cgggttagag
    15961 aaggttgcca tatcttcccg ggtggtactt aactgcccga tcaacggata ttgtttgctg
    16021 gcaatgagca gtttggttaa gtcacgggca gttgaaacgt tatgcaccga caatccggta
    16081 ggttcaacaa agcgcgtgtt gttcattccg agcgatttcg ctttcgcatt cattgcctta
    16141 ataaaggctt tgtaaccacc gggataatgg tgcgcaaggc ttgccgccgc gcggttttct
    16201 gaagacatca gcgccagcaa cagcatatct ttacggctga tttcgctgtt cagtcgtacg
    16261 cgcgaataga cccctttcat ctccggcgtc tggctgatat ccacttttag tttttcatcc
    16321 agcggcagtc gtgcatccag cacaaccatc gcggtcatta atttgctgat agacgcaatc
    16381 ggacgcacca gatccgggtg gttcgaatag atcactttgt tggtattcag atcaacaatc
    16441 atcgcgctac cggaggcaat ttccggttgt gacgcggtgg tagcggctgc cgttttcgca
    16501 acggcctgcg gtgcaaaagg cacagccagc atcagggcca ggctaaataa agaaactcga
    16561 aatttcggca tgatgagcat tcagatagtg gttcacgcgc acgggttgcg caccgccgga
    16621 gtaaggattt actgaggcta gcgacgccat cataacgagc aaaaagtgcg atcgtcaaag
    16681 aagaatcgtg aggaaatgct gcattgctga catttacgcc agcaatgcag cgcaaaagaa
    16741 ctttctagaa caaacgataa ccgtagcccc atagtataac ggtaagggcg agcagcactt
    16801 ccagcaccag cacgcctatc gccagtgtcg aactggagaa gctaagtcct tcctctttgt
    16861 tgatattcag gaagctcgga ataccgagat acagcaggta gccggtgtaa aacagcgcca
    16921 ccgtgccgac cagcgcacac aaccagacca gcgggtaaag cgccaccaga ccgcttaaaa
    16981 acagcggagt tgcaacgtag cccgcgaaga ccatgcagtg cgcaagtgac ggacgctgcg
    17041 gataattacg cgccatccac cagatgaccc gccccatcac cgcaacccca gccagcataa
    17101 cgccgtaaaa caagacagcc agcgccagtc cggtaaacca ggataacttc aggatagtgc
    17161 catcgccaaa attccagcca atctgtgtag tgccaatgaa ggcgcaaatc accggaatcg
    17221 ccgccatcag caaaacgtgg tgggtgtaat ggtgagaaat cgtttcgttt tcgcgattaa
    17281 tcacctgcat ttcacgatcg ggatgggaaa acagtcccca gacatggctc ataccgcccc
    17341 cttgttgtga gttcatgaac ctgacagttc aagtataagt cagcttgtga ttattttttg
    17401 ttcgctcatg taatttgcac cgtctgatgt atgtcctttt tcagcgtgaa tgattcacag
    17461 ggtgtatgct tacaggcaag caaagggaga cagactgtcc tatggatctc aatacactca
    17521 tctcacaata tggttatgcc gcgctggtga tcggtagcct ggcggaaggt gaaaccgtga
    17581 ctttgctggg aggcgttgcg gcacatcagg ggctattaaa gttcccgctg gtggtacttt
    17641 ctgtggcgct tggcggcatg attggcgacc aggtgcttta tctgtgcggg cggcgctttg
    17701 gcggcaagct gttacgccgt ttctcgaaac atcaggataa aattgagcgg gcgcagaaac
    17761 ttatccaacg ccatccgtat ctgtttgtca ttggtacgcg ttttatgtat ggctttcggg
    17821 tgattggccc gacgctgatt ggtgccagcc agctgccgcc gaaaatcttt ctgccgctga
    17881 atattctcgg cgcatttgcc tgggcgctga tttttaccac tatcggttac gctggtgggc
    17941 aggtgattgc gccgtggttg cacaatctcg accagcattt gaagcactgg gtctggttga
    18001 ttctggtcgt ggttctggtg gtaggcgtgc gctggtggct gaaacggcgc gggaagaaaa
    18061 agccggatca tcaggcgtaa aaccattgcc ctggataagg tgttcgcgcc gcatccgaca
    18121 tccggtgcac catgcctgat gcgacgctgg cgtgtcttat ctggcctaac ccaatgaaat
    18181 aaccacgcac ctcactgcgc tattctgggt tgaactgcgg attcgccaac ataaagccgc
    18241 catccactat caacgactgc ccggtggtgt aatttgcgcc ctccgaacaa agccatacca
    18301 ccaggctggc aatctcatgc gttgtgccaa aacgtcgcaa gggaatcgaa ggctccgcgt
    18361 caggcttcac gtcgccgcca tccatgccgt tcattggcgt ggcgatcgcc ccaggtgcga
    18421 ctgcgttcac caaaatctta tgcctgacca gctccagcgc catcgttttg gttaac
//
LOCUS       DAMZFT010000094        18342 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.094, whole genome shotgun sequence.
ACCESSION   DAMZFT010000094 DAMZFT010000000
VERSION     DAMZFT010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18342)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18342)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18342
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.094"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..582
                     /locus_tag="PN977_003420"
     CDS             <1..582
                     /locus_tag="PN977_003420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HDI6043423.1"
                     /translation="GHKFGLAPLGCGWVIWRDEEALPQELVFNVDYLGGQIGTFAINF
                     SRPAGQVIAQYYEFLRLGREGYTKVQNASYQVAAYLADEIAKLGPYEFICTGRPDEGI
                     PAVCFKLKDGEDPGYTLYDLSERLRLRGWQVPAFTLGGEATDIVVMRIMCRRGFEMDF
                     AELLLEDYKASLKYLSDHPKLQGIAQQNSFKHT"
     gene            738..2273
                     /gene="gadC"
                     /locus_tag="PN977_003421"
     CDS             738..2273
                     /gene="gadC"
                     /locus_tag="PN977_003421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance gamma-aminobutyrate antiporter
                     GadC"
                     /protein_id="HDI6043424.1"
                     /translation="MATSVQTGKAKQLTLLGFFAITASMVMAVYEYPTFATSGFSLVF
                     FLLLGGILWFIPVGLCAAEMATVDGWEEGGVFAWVSNTLGPRWGFAAISFGYLQIAIG
                     FIPMLYFVLGALSYILKWPALNEDPITKTIAALIILWALALTQFGGTKYTARIAKVGF
                     FAGILLPAFILIALAAIYLHSGAPVAIEMDAKTFFPDFSKVGTLVVFVAFILSYMGVE
                     ASATHVNEMSNPGRDYPLAMLLLMVAAICLSSVGGLSIAMVIPGNEINLSAGVMQTFT
                     VLMSHVAPEIEWTVRVISALLLLGVLAEIASWIVGPSRGMYVTAQKNLLPAAFAKMNK
                     NGVPVTLVISQLVITSIALIILTNTGGGNNMSFLIALALTVVIYLCAYFMLFIGYIVL
                     VLKHPDLKRTFNIPGGKGVKLVVAIVGLLTSIMAFIVSFLPPDNIQGDSTDMYVELLV
                     VSFLVVLALPFILYAVHDRKGKGNTGVTLEPINSQNAPKGHFFLHPRARSPHYIVMND
                     KKH"
     gene            2404..3723
                     /locus_tag="PN977_003422"
     CDS             2404..3723
                     /locus_tag="PN977_003422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 10 protein"
                     /protein_id="HDI6043425.1"
                     /translation="MDICSRNEKLAIRRPAILVALALLLCSCKSTPPESMVTPPAGSK
                     PPATTQQSSQPMRGIWLATVSRLDWPPVSSVNISNPTSRARVQQQAMIDKLDHLQRLG
                     INTVFFQVKPDGTALWPSKILPWSDLMTGKIGENPGYDPLQFMLDEAHKRGMKVHVWF
                     NPYRVSVNTKPGTIRELNSTLSQQPASVYVQHRDWIRTSGDRFVLDPGIPEVQDWITS
                     IVAEVVSRYPVDGVQFDDYFYTESPGSRLNDNETYRKYGGAFASKADWRRNNTQQLIA
                     KVSHTIKSIKPEVEFGVSPAGVWRNRSHDPLGSDTRGAAAYDESYADTRRWVEQGLLD
                     YIAPQIYWPFSRSAARYDVLAKWWADVVKPTRTRLYIGIAFYKVGEPSKIEPDWIING
                     GVPELKKQLDLNDAVPEISGTILFREDYLNKPQTQQAVSYLQSRWGS"
     gene            4020..4061
                     /locus_tag="PN977_003423"
     CDS             4020..4061
                     /locus_tag="PN977_003423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6043426.1"
                     /translation="MVQLVDLARCVSF"
     gene            4099..5481
                     /gene="dosC"
                     /locus_tag="PN977_003424"
     CDS             4099..5481
                     /gene="dosC"
                     /locus_tag="PN977_003424"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DosC"
                     /protein_id="HDI6043427.1"
                     /translation="MEMYFKRMKDEWTGLVEQADPLIRAKAAEIAVAHAHYLSIEFYR
                     IVRIDPHAEEFLSNEQVERQLKSAMERWIINVLSAQVDDVERLIQIQHTVAEVHARIG
                     IPVEIVEMGFRVLKKILYPVIFSSDYSAAEKLQVYHFSINSIDIAMEVMTRAFTFSDS
                     SASKEDENYRIFSLLENAEEEKERQIASILSWEIDIIYKVLLDSDLGSSLPLSQADFG
                     LWFNHKGRHYFSGIAEVGHISRLIQDFDGIFNQTMRNTRNLNNRSLRVKFLLQIRNTV
                     SQIITLLRELFEEVSRHEVGMDVLTKLLNRRFLPTIFKREIAHANRTGTPLSVLIIDV
                     DKFKEINDTWGHNTGDEILRKVSQAFYDNVRSSDYVFRYGGDEFIIVLTEASENETLR
                     TAERIRSRVEKTKLKAANGEDIALSLSIGAAMFNGHPDYERLIQIADEALYIAKRRGR
                     NRVELWKASL"
     gene            5506..7905
                     /gene="dosP"
                     /locus_tag="PN977_003425"
                     /gene_synonym="pdeO"
     CDS             5506..7905
                     /gene="dosP"
                     /locus_tag="PN977_003425"
                     /gene_synonym="pdeO"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-sensing cyclic-di-GMP phosphodiesterase"
                     /protein_id="HDI6043428.1"
                     /translation="MKLTDADNAADGIFFPALEQNMMGAVLINENDEVMFFNPAAEKL
                     WGYKREEVIGNNIDMLIPRDLRPAHPEYIRHNREGGKARVEGMSRELQLEKKDGSKIW
                     TRFALSKVSAEGKVYYLALVRDASVEMAQKEQTRQLIIAVDHLDRPVIVLDPERHIVQ
                     CNRAFTEMFGYCISEASGMQPDSLLNIPEFPADNRIRLQQLLWKTARDQDEFLLLTRT
                     GEKIWIKASISPVYDVLAHLQNLVMTFSDITEERQIRQLEGNILAAMCSSPPFHEMGE
                     IICRNIESVLNESHVSLYALRNGMPIHWASSSHGTEVQNAQSWSATIRQRDGAPAGIL
                     QIKTSSGAETSAFIERVADISQHMAALALEQEKSRQHIEQLIQFDPMTGLPNRNNLHN
                     YLDDLVDKAVSPVVYLIGVDHIQDVIDSLGYAWADQALLEVVNRFREKLKPDQYLCRI
                     EGTQFVLVSLENDVSNITQIADELRNVVSKPIMIDDKPFPLTLSIGISYDVGKNRDYL
                     LSTAHNAMDFIRKNGGNGWQFFSPAMNEMVKERLVLGAALKEAISNNQLKLVYQPQIF
                     AETGELYGIEALARWHDPLHGHVPPSRFIPLAEEIGEIENIGRWVIAEACRQLAEWRS
                     QNIHIPALSVNLSALHFRSNQLPNQVSDAMQAWGIDGHQLTVEITESMMMEHDTEIFK
                     RIQILRDMGVGLSVDDFGTGFSGLSRLVSLPVTEIKIDKSFVDRCLTEKRILALLEAI
                     TSIGQSLNLTVVAEGVETKEQFEMLRKIHCRVIQGYFFSRPLPAEEIPGWMSSVLPLK
                     I"
     gene            8163..8744
                     /gene="ddpX"
                     /locus_tag="PN977_003426"
     CDS             8163..8744
                     /gene="ddpX"
                     /locus_tag="PN977_003426"
                     /EC_number="3.4.13.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine dipeptidase"
                     /protein_id="HDI6043429.1"
                     /translation="MSDTTELVDLAVTFPDLEIELKYACADNITGKAIYQQARCLLHK
                     DAITALAKSISIAQLSGLQLVIYDAYRPQQAQAMLWQACPDPQYVVDVAVGSNHSRGT
                     AIDLTLRDEHGNILDMGAEFDEMHERSHAYHPSVPPAAQRNRLLLNAIMTGGGFVGIP
                     SEWWHFELPQAASYPLLADQFTCFISPGTQHAS"
     gene            8758..10308
                     /locus_tag="PN977_003427"
     CDS             8758..10308
                     /locus_tag="PN977_003427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDI6043430.1"
                     /translation="MKRSILFRPTLLAIVLATTMPVAHAAVPKDMLVIGKAADPQTLD
                     PAVTIDNNDWTVTYPSYQRLVQYKTDGDKGSTDVEGDLASSWKASDDQKEWTFTLKND
                     AKFADGTPVTAEAVKLSFERLLKIGQGPAEAFPKDLKIDAPDEHTVRFTLSQPFAPFL
                     YTLANDGASIINPAVLKEHAADDARGFLAQNTAGSGPFMLKSWQKGQQLVLVPNPHYP
                     GNKPNFKRVSVKIIGESASRRLQLSRGDIDIADALPVDQLNALKQENKVNVAEYPSLR
                     VTYLYLNNSKAPLNQADLRRAISWSTDYQGMVNGILSGNGKQMRGPIPEGMWGYDATA
                     MQYNHDETKAKAEWDKVTSKPTSLTFLYSDNDPNWEPIALATQSSLNKLGINVKLEKL
                     ANATMRDRVGKGDYDIAIGNWSPDFADPYMFMNYWFESDKKGLPGNRSFYENSEVDKL
                     LRNALATTDQTQRTRDYQQAQKIVIDDAAYVYLFQKNYQLAMNKEVKGFVFNPMLEQV
                     FNINTMSK"
     gene            10310..11332
                     /locus_tag="PN977_003428"
     CDS             10310..11332
                     /locus_tag="PN977_003428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDI6043431.1"
                     /translation="MTFWSILRQRCWGLVLVVAGVCVITFIISHLIPGDPARLLAGDR
                     ASDAIVENIRQQLGLDQPLYVQFYRYVSDLFQGDLGTSIRTGRPVLEELRIFFPATLE
                     LAFCALLLALLIGIPLGILSAVWRNRWLDHLVRIMAITGISTPAFWLGLGVIVLFYGH
                     LQILPGGGRLDDWLDPPTHVTGFYLLDALLEGNGEVFFNALQHLILPALTLAFVHLGI
                     VARQIRSAMLEQLSEDYIRTARASGLPGWYIVLCYALPNALIPSITVLGLALGDLLYG
                     AVLTETVFAWPGMGAWVVTSIQALDFPAVMGFAVVVSFAYVLVNLVVDLLYLWIDPRI
                     GRGGGE"
     gene            11329..12225
                     /locus_tag="PN977_003429"
     CDS             11329..12225
                     /locus_tag="PN977_003429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D,D-dipeptide ABC transporter permease"
                     /protein_id="HDI6043432.1"
                     /translation="MMLSEETSAVRPPKQTRFNGAKLVWMLKGSPLTVTGAVIIVLML
                     LMMIFSPWLATHDPNAIDLTARLLPPSAAHWFGTDEVGRDLFSRVLVGSQQSILAGLV
                     VVAIAGMMGSLLGCLSGVLGGRADAIIMRIMDIMLSIPSLVLTMALAAALGPSLFNAM
                     LAIAIVRIPFYVRLARGQTLVVRQYTYVQAAKTFGASRWHLINWHILRNSLPPLIVQA
                     SLDIGSAILMAATLGFIGLGAQQPSAEWGAMVANGRNYVLDQWWYCAFPGAAILLTAV
                     GFNLFGDGIRDLLDPKAGGKQS"
     gene            12222..13208
                     /locus_tag="PN977_003430"
     CDS             12222..13208
                     /locus_tag="PN977_003430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDI6043433.1"
                     /translation="MTQPVLDIQQLHLSFPGFNGDVHALNNVSLKINRGEIVGLVGES
                     GSGKSVTAMLIMRLLPTGSYCVHRGHISLLGEDVLNAREKQLRQWRGARVAMIFQEPM
                     TALNPTRRIGLQMMDVIRHHQPISRREARAKAIALLEEMQIPDAVEVMSRYPFELSGG
                     MRQRVMIALAFSCEPQLIIADEPTTALDVTVQLQVLRLLKHKARASGTAVLFISHDMA
                     VVSQLCDSVYVMYAGSVIESGVTADVIHHPRHPYTIGLLQCAPEHGIPRQPLPAIPGT
                     VPNLTHLPDGCAFRDRCYAAGTQCENVPALTACGDNNHRCACWYPQQEVISV"
     gene            13201..14127
                     /locus_tag="PN977_003431"
     CDS             13201..14127
                     /locus_tag="PN977_003431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDI6043434.1"
                     /translation="MSDTLLTLRDVHINFPARKNWLGKTTEHVHAINGIDLQIRRGET
                     LGIVGESGCGKSTLAQLLMGMLQPSHGQYIRSGSQRIMQMVFQDPLSSLNPRLPVWRI
                     ITEPLWIAKRSSEQQRRALAEELAVQVGIRPEYLDRLPHAFSGGQRQRIAIARALSSQ
                     PDVIVLDEPTSALDISVQAQILNLLVTLQENRELTYVLISHNVSVIRHMSDRVAVMYL
                     GQIVELGDAQQVLTAPAHPYTRLLLDSLPAIDKPLEEEWALRKTDLPGNRTLPQGCFF
                     CERCPLATHGCEVRQSLTIREDGREIRCWRAL"
     gene            complement(14183..14614)
                     /gene="osmC"
                     /locus_tag="PN977_003432"
     CDS             complement(14183..14614)
                     /gene="osmC"
                     /locus_tag="PN977_003432"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin OsmC"
                     /protein_id="HDI6043435.1"
                     /translation="MTIHKKGQAHWEGDIKRGKGTVSTESGVLNQQPYGFNTRFEGEK
                     GTNPEELIGAAHAACFSMALSLMLGEAGFTPTSIDTTADVSLDKVDAGFAITKIALKS
                     EVAVPGIDASTFDGIIQKAKAGCPVSQVLKAEITLDYQLKS"
     gene            14959..15174
                     /gene="bdm"
                     /locus_tag="PN977_003433"
     CDS             14959..15174
                     /gene="bdm"
                     /locus_tag="PN977_003433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310112.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm-dependent modulation protein"
                     /protein_id="HDI6043436.1"
                     /translation="MFTYYQAENSTAEPALVNAIEQGLRAEHGVVTEDDILMELTKWV
                     EASDNDILSDIYQQTINYVVSGQHPTL"
     gene            15276..15413
                     /gene="sra"
                     /locus_tag="PN977_003434"
     CDS             15276..15413
                     /gene="sra"
                     /locus_tag="PN977_003434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary-phase-induced ribosome-associated
                     protein"
                     /protein_id="HDI6043437.1"
                     /translation="MKSNRQARHILGLDHKISNQRKIVTEGDKSSVVNNPTGRKRPAE
                     K"
     gene            15570..17267
                     /gene="maeA"
                     /locus_tag="PN977_003435"
     CDS             15570..17267
                     /gene="maeA"
                     /locus_tag="PN977_003435"
                     /EC_number="1.1.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HDI6043438.1"
                     /translation="MEPKTKKQRSLYIPYAGPVLLEFPLLNKGSAFSMEERRNFNLLG
                     LLPEVVETIEEQAERAWIQYQGFKTEIDKHIYLRNIQDTNETLFYRLVNNHLDEMMPV
                     IYTPTVGAACERFSEIYRRSRGVFISYQNRHNMDDILQNVPNHNIKVIVVTDGERILG
                     LGDQGIGGMGIPIGKLSLYTACGGISPAYTLPVVLDVGTNNQQLLNDPLYMGWRNPRI
                     TDDEYYEFVDEFIQAVKQRWPDVLLQFEDFAQKNAMPLLNRYRNEICSFNDDIQGTAA
                     VTVGTLIAASRAAGGQLSEKKIVFLGAGSAGCGIAEMIIAQTQREGLSEETARQKVFM
                     VDRFGLLTDKMPNLLPFQTKLVQKRENLSDWDTDSDVLSLLDVVRNVKPDILIGVSGQ
                     TGLFTEEIIREMHKHCPRPIVMPLSNPTSRVEATPQDIIAWTEGNALVATGSPFNPVV
                     WKDKIYPIAQCNNAFIFPGIGLGVIASGASRITDEMLMSASETLAQYSPLVLNGEGLV
                     LPELKDIQKVSRAIAFAVGKMAQQQGVAVKTSAEALQQAIDDNFWQAEYRDYRRTSI"
     gene            17401..>18342
                     /gene="adhP"
                     /locus_tag="PN977_003436"
     CDS             17401..>18342
                     /gene="adhP"
                     /locus_tag="PN977_003436"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
                     /protein_id="HDI6043439.1"
                     /translation="MKAAVVTKDHHVDVTDKTLRSLKHGEALLKMECCGVCHTDLHVK
                     NGDFGDKTGVILGHEGIGVVAEVGPGVTSLKPGDRASVAWFYEGCGHCEYCNSGNETL
                     CRSVKNAGYSVDGGMAEECIVVADYAVKVPDGLDSAAASSITCAGVTTYKAVKLSKIR
                     PGQWIAIYGLGGLGNLALQYAKNVFNAKVIAIDVNDEQLKLATEMGADLAINSRTEDA
                     AKIVQEKTGGAHAAVVTAVAKAAFNSAVDAVRAGGRVVAVGLPPESMSLDIPRLVLDG
                     IEVVGSLVGTRQDLTEAFQFAAEGKVVPKVALRPLADI"
BASE COUNT         4554 a         4529 c         4707 g         4552 t
ORIGIN      
        1 ggccataaat tcggtctggc tccgctgggc tgcggctggg ttatctggcg tgatgaagaa
       61 gcgctgccgc aggaactggt gttcaacgtt gactatctgg gtggtcagat tgggactttc
      121 gccatcaact tctcccgccc ggcaggtcag gtgattgcac agtactatga attcctgcgc
      181 cttggccgtg aaggctatac caaagtacag aacgcttcct accaggttgc tgcctatctg
      241 gcggatgaaa tcgccaaact ggggccgtat gagttcatct gtaccggtcg cccggacgaa
      301 ggcatcccgg cggtttgctt caaactgaaa gatggtgaag acccgggata caccctctac
      361 gacctctctg aacgtctgcg cctgcgcggc tggcaggttc cggccttcac tctcggcggt
      421 gaagccactg acatcgtggt gatgcgcatt atgtgtcgtc gcggcttcga aatggacttt
      481 gctgaactgt tgctggaaga ctacaaagcc tccctgaaat atctcagcga tcacccgaaa
      541 ctgcagggta ttgcccagca gaacagcttt aaacatacct gataacgttt aacggtaacg
      601 gtgtcccgaa acacacccgt ttcgggacaa tttccaaagt ctgttcactg gcattagcaa
      661 cggaaaatat tgttctgaat acgcttcaga acaaaacagg tgcggttccg acaggaatac
      721 cgttttaggg ggataatatg gctacatcag tacagacagg taaagctaag cagctcacat
      781 tactcggatt ctttgccata acggcatcga tggtaatggc tgtttatgaa taccctacct
      841 tcgcaacatc gggcttttca ttagtcttct tcctgctatt aggcgggatt ttatggttta
      901 ttcccgtggg actttgtgct gcggaaatgg ctaccgtcga cggctgggaa gaaggtggcg
      961 tcttcgcctg ggtatcaaat actttggggc cgagatgggg atttgcagcg atctcatttg
     1021 gttatctgca aatcgccatt ggttttattc cgatgctcta tttcgtgtta ggggcactct
     1081 cctacatcct gaaatggcca gcgctgaatg aagaccccat taccaaaact attgcagcac
     1141 tcatcattct ttgggcgctg gcattaacgc agtttggtgg cacgaaatac acggcgcgca
     1201 ttgctaaagt tggcttcttc gccggtatcc tgttacctgc atttattttg atcgcattag
     1261 cggctattta tctgcactcc ggtgcccccg ttgctatcga aatggatgcg aagaccttct
     1321 tccctgactt ctctaaagtg ggcaccctgg ttgtatttgt tgccttcatt ttgagttata
     1381 tgggcgttga agcttccgca acccacgtca atgaaatgag caaccccggg cgcgactatc
     1441 cactggctat gttactgctg atggtagcgg caatctgctt aagctctgtt ggcggtttgt
     1501 ctattgcgat ggtcattccg ggtaatgaaa tcaacctctc cgcaggggta atgcaaacct
     1561 ttaccgttct gatgtcacat gtggcaccgg aaattgagtg gacggttcgc gtgatctccg
     1621 cactgctgtt gctgggtgtt ctggcggaaa tcgcttcctg gattgttggt ccttctcgcg
     1681 ggatgtatgt cacagcgcag aaaaacctgc tgccagcagc attcgctaaa atgaacaaaa
     1741 atggcgtacc ggtaacgctg gtcatttcgc agctggtgat tacttctatc gcgttgatca
     1801 tcctcaccaa taccggtggc ggtaacaaca tgtccttcct gatcgcactg gcactgacgg
     1861 tggtgattta tctgtgtgct tatttcatgc tgtttattgg ctacattgtg ttggttctta
     1921 aacatcctga cttaaaacgc acatttaata tccctggtgg taaaggggtg aaactggtcg
     1981 tggctattgt cggtctgctg acttcaatta tggcgtttat tgtttccttc ctgccgccgg
     2041 ataacatcca gggtgactct accgatatgt atgttgaatt actggtcgtg agtttcctgg
     2101 tggtacttgc cctgcccttt attctctatg ctgttcatga tcgtaaaggc aaagggaata
     2161 ccggcgttac tctggagcca atcaacagtc agaacgcacc aaaaggtcac ttcttcctgc
     2221 acccgcgtgc acgttcacca cactatattg tgatgaatga caagaaacac taaccatgtc
     2281 tgaatggtta taagacaagg gagcgataat tcatcgctcc ctttttcgtg cttgctgtcg
     2341 tattgactta accgggataa gtacgagaat gagcgcacat ctgtttaccg gaaaccagca
     2401 catatggata tctgctcccg aaacgagaaa ttagcgatta gaagaccagc gatactggtt
     2461 gcactggcac ttttactgtg tagttgtaaa agcacgcctc cagagtccat ggtgacacca
     2521 ccagcgggtt caaagccacc agccacgacg caacaatcgt cacaaccgat gcgtggcatc
     2581 tggctggcca cggtgtctcg cctcgactgg ccaccggttt cctcggttaa cattagtaac
     2641 cccaccagcc gggcccgtgt acaacaacag gcgatgatcg acaaactgga tcatctgcaa
     2701 cgtctcggca taaacacggt ctttttccag gtcaagccag acggtaccgc cctgtggcca
     2761 tcgaaaattt tgccgtggtc cgatcttatg accggtaaga ttggtgaaaa tccgggttac
     2821 gatccgctgc aattcatgct cgacgaagcc cacaagcgtg ggatgaaagt acacgtctgg
     2881 tttaacccct atcgcgtatc ggttaatacg aagcccggta ctatcaggga actgaatagc
     2941 accctgtctc aacaaccggc gagcgtctat gtgcaacacc gcgactggat cagaacgtcc
     3001 ggcgatcgct ttgtcctcga cccgggcata cctgaggttc aggactggat cacatcaata
     3061 gttgcagaag tggtttcccg ctatccggta gatggcgtgc agtttgacga ctatttctat
     3121 actgagtcgc caggttcacg gctaaatgat aatgaaacgt accgcaaata cggcggcgca
     3181 tttgcgtcaa aagcagactg gcggcgcaac aatactcagc agttaattgc gaaggtatcg
     3241 cacaccatta aaagcattaa gccggaagtc gaatttggcg ttagcccggc aggcgtgtgg
     3301 cgtaaccgat cacacgatcc gctcggttcc gatacccgag gcgcggcagc ctatgacgaa
     3361 tcctacgctg atacccgtcg atgggtggaa caagggttgc tggattacat tgctccccaa
     3421 atttactggc cattctcacg gagtgccgcg cgttatgacg tgttggcaaa atggtgggcg
     3481 gatgtcgtta aaccgaccag gacccgcctg tatatcggta tcgccttcta taaagtgggt
     3541 gaaccttcaa agatagagcc agactggata attaacggcg gcgtaccgga actgaaaaag
     3601 cagctcgatc ttaacgatgc cgtgccagaa attagcggca caatcttgtt ccgtgaggac
     3661 tatctgaata agccacagac tcaacaagcg gtcagctatc tgcaaagtcg ttggggcagt
     3721 taaactccca ttcttacttt gtaacaagcc ggtgctttcc ccccggcttg ttaccctctt
     3781 gaaatatccc actagtaatc atgcttactt aagtcaaatt aactacgcaa ttaaccacac
     3841 ttagttaaac agtcataaat cccctaataa aaatgtatga acatgaaaaa acatacgata
     3901 ttcgctgtca ccgaaaaata attacgatgg gctatactct tctgaagaac agtagcgtag
     3961 attgaaactg tcctaagtcg cttatttgtt ttcagtatat cttcatattt caggagagta
     4021 tggtgcagtt agtcgatctc gcacgttgcg tttcgttttg attccgtctg ctttcccagc
     4081 cttataaggg tgtgaattat ggagatgtat tttaaaagaa tgaaagatga gtggaccggg
     4141 cttgtcgaac aagcagatcc gctcattcgt gctaaagccg cggaaattgc cgttgcgcat
     4201 gcccattatc tgagtattga gttttaccga attgtccgta tcgacccgca tgccgaagaa
     4261 ttcttgagta atgaacaagt tgagcggcag ttgaaaagtg cgatggaacg ctggattatt
     4321 aacgtgcttt ctgcccaggt tgacgatgtc gaaaggctaa tacaaatcca gcataccgtc
     4381 gcggaagtgc atgcccgcat aggaattccg gtagaaattg tcgagatggg gtttcgggtg
     4441 ctgaaaaaaa tcctctatcc ggtcatcttc tcttcggatt attccgccgc ggaaaaactt
     4501 caggtctacc atttctcgat taacagtatt gatatcgcta tggaagtgat gacccgcgcg
     4561 tttaccttta gtgacagtag tgcctcaaag gaagatgaaa actatcgtat cttctcgtta
     4621 ctggaaaatg ccgaagaaga aaaagaacgg caaatagcct caatactttc atgggaaata
     4681 gatattatct ataaagtcct gctggattct gatttaggca gtagtttgcc tttaagccag
     4741 gctgattttg gcctgtggtt taaccataaa ggtcgacatt attttagcgg catagccgaa
     4801 gtgggccata tctcccgtct gattcaggat ttcgacggta ttttcaatca aaccatgcgt
     4861 aacaccagga atttgaataa cagaagttta cgggtgaaat ttttattaca gataagaaat
     4921 accgtatcgc aaattattac cttgttgcgt gaattgtttg aagaagtatc gcgccacgaa
     4981 gtcggtatgg atgtactgac gaaattactt aaccgccgtt tcctgccgac gatcttcaaa
     5041 cgcgaaattg cccatgccaa ccggaccggt acaccgctgt cagtgctgat tattgacgtt
     5101 gataaattca aagagattaa cgatacgtgg ggccataaca ctggcgatga aattctgcgt
     5161 aaagtctctc aggcctttta tgacaacgtc cgcagtagtg attatgtttt ccgctacggg
     5221 ggcgatgaat ttatcattgt tttgactgaa gcttcggaaa acgaaacgtt acgtaccgca
     5281 gaacgtattc gcagtcgggt ggagaaaacc aaactgaaag ccgcaaacgg cgaggatatt
     5341 gccctctcac tttccatcgg tgccgccatg tttaatggtc atcctgacta tgagcgcctc
     5401 attcaaatag ccgatgaagc tctgtatatc gccaaaagac gaggtagaaa ccgtgttgaa
     5461 ctctggaaag ccagtcttta gatgcgtcag gatgcagagg taattatgaa gctaaccgat
     5521 gcggataatg ccgccgatgg catttttttc cccgcccttg agcaaaatat gatgggtgcg
     5581 gtgttaatta acgaaaatga tgaagtgatg tttttcaacc ccgccgcaga gaagctctgg
     5641 gggtacaaac gtgaagaagt cattggcaat aacattgata tgctgattcc gcgggatttg
     5701 cgtcctgcgc atcctgaata cattcgtcat aaccgtgaag gcggtaaagc gcgcgtcgag
     5761 gggatgagtc gggagctgca gctggagaaa aaagacggca gtaaaatctg gacccgtttt
     5821 gcgctatcga aagtgagcgc cgaggggaaa gtttattacc tggcgctggt acgggatgcc
     5881 agcgtagaaa tggcgcaaaa agaacagacc cgacaattga ttattgccgt tgaccatctc
     5941 gaccgaccgg tgattgtcct cgatccggaa cgccatattg tgcagtgcaa tcgcgcattt
     6001 accgaaatgt ttggttactg cattagcgaa gccagcggta tgcagcccga ttcactcctt
     6061 aacattcctg aattccctgc cgataaccgc attcgtttac aacagttgct atggaaaacc
     6121 gcccgcgatc aggatgaatt tctgctgttg acgcgcaccg gtgaaaaaat ctggattaaa
     6181 gcctctatca gcccggttta tgacgtgctc gcgcatctgc agaacctggt aatgaccttc
     6241 tcggatatca ccgaagaacg gcaaatccgc cagcttgaag gcaatattct cgccgccatg
     6301 tgcagcagcc cgccatttca tgaaatgggg gaaatcattt gtcgtaacat cgaatctgta
     6361 ctcaacgaat cgcatgtttc gctgtacgcg ctgcgcaacg ggatgccgat acactgggcg
     6421 tcatcttcgc acggtacaga agttcaaaat gcgcaaagct ggtcagcgac cattcgtcag
     6481 cgtgatggcg cgccggcggg gatcctgcaa attaaaacct cgtcaggagc agaaaccagc
     6541 gcctttatcg aacgcgtggc agatatcagc cagcatatgg ccgcgctggc gctggaacag
     6601 gaaaaaagcc gtcagcatat tgaacaactc atccaatttg atccgatgac cggtctgcca
     6661 aatcgcaata acctgcacaa ttacctcgat gacctggtcg acaaagccgt ctctccagtg
     6721 gtgtatctca tcggtgttga ccatattcag gatgtgattg atagccttgg ctatgcgtgg
     6781 gccgatcagg cattgctgga agtggtcaat cgctttcgtg aaaaactcaa accggatcaa
     6841 tatctctgtc gtatcgaagg tacgcagttt gtcctcgtga gcctcgaaaa cgacgtcagt
     6901 aacattacgc agatcgccga tgagctacgg aatgtggtca gcaagccgat aatgattgac
     6961 gataaaccat tcccgctcac cttgagcatt ggcatcagct acgacgtggg taaaaaccgc
     7021 gattacctgc tctccactgc tcacaatgca atggatttta ttcgcaaaaa tggcggtaac
     7081 ggctggcagt tcttcagccc ggcgatgaac gaaatggtaa aagagcgttt ggttttaggc
     7141 gcggcgctga aagaagcaat tagcaataac caactgaaac tggtttacca gccacaaatc
     7201 ttcgcagaaa cgggtgaact gtacggcatc gaagcccttg ctcgctggca cgatcccctg
     7261 catggtcatg tgcccccttc acggtttatt cctctcgcag aagagatcgg tgaaatcgaa
     7321 aatattggac gctgggtcat cgcggaagct tgccgtcagt tagcagaatg gcgtagccag
     7381 aatattcata tcccggcgtt atccgtgaac ttgtcggcgc tgcactttcg cagtaatcag
     7441 ctgcctaatc aggtgtctga tgcaatgcaa gcctggggta ttgacggcca ccagctgacg
     7501 gtagaaatca cggaaagcat gatgatggaa cacgataccg aaatctttaa gcgcattcag
     7561 atcctgcgcg atatgggcgt gggtttatcg gtagatgatt ttggcacggg cttttccgga
     7621 ttatcccgct tagtcagtct tccagtaacg gaaatcaaaa ttgacaaaag ttttgtcgat
     7681 cgttgtctga ccgaaaaacg catccttgcc ttacttgaag ccattaccag cattgggcaa
     7741 agccttaatt taaccgtcgt ggcggaaggc gtcgaaacca aagagcaatt tgagatgcta
     7801 cgcaagatcc actgtcgcgt tattcaggga tatttctttt cccgccccct acccgccgaa
     7861 gaaattccag gctggatgag cagcgtgtta ccgctgaaaa tctgacattt tcctctcgcc
     7921 cgcactcgcg ggttttcttt taacgtgaca ctgtcactta aatactgtga tttcagctac
     7981 gattccggga gattccttcc ttaacttccc ctcttgcacc aaaactgcac tacgctgcac
     8041 aaatgttgat caaaagttaa aaaatataac tttaagccag cattctggcg cttatcccgg
     8101 catggcatga gatctgcata agcggaaagc gcagcaattt ttgtcttata caggaaccgt
     8161 ttatgtcgga taccaccgaa ctggttgatt tagccgtgac cttccctgat ctggagatcg
     8221 aactgaaata cgcctgcgct gataacatca caggtaaagc tatttatcag caagcgcgtt
     8281 gtctgttaca caaggatgcg attaccgcgc tggcgaaaag tatcagtatc gcccagctgt
     8341 caggcttaca actggtgatt tacgatgcgt atcgcccaca acaagcacag gcgatgttgt
     8401 ggcaagcctg cccagacccg caatatgttg ttgatgtggc ggtcggttct aatcacagcc
     8461 gtggcacggc gatcgacctg acgcttcgtg atgagcacgg gaacatactg gatatgggtg
     8521 cagagttcga tgaaatgcac gaacgttccc atgcttatca cccttccgtc ccgccagccg
     8581 ctcagcgcaa tcggctgttg ctgaatgcga ttatgactgg cggtggcttt gtcggtatcc
     8641 cgagcgaatg gtggcacttc gaattacccc aggcagcgag ttacccacta ctcgctgatc
     8701 aattcacctg ttttatatcc cctggcacac agcacgccag ttaatcagga aactgtcatg
     8761 aagagatcga ttttgtttcg tcctacattg ctcgcgatcg tccttgccac aacaatgccg
     8821 gttgcgcacg ccgccgtacc gaaagatatg ctggtgatcg gtaaagctgc cgacccacaa
     8881 accctcgacc cagcggtgac aattgataat aacgactgga cagtgaccta cccgtcttat
     8941 cagcgactgg ttcagtacaa aacggacggt gataaaggct caaccgacgt tgaaggcgat
     9001 ctggcaagta gctggaaagc gtctgacgat caaaaagagt ggacgttcac cctgaaaaac
     9061 gacgctaaat ttgccgatgg cacacctgtc actgccgaag cagtaaaact ctcttttgag
     9121 cggttactaa aaatcggcca ggggccagca gaagcatttc ccaaagattt aaagattgat
     9181 gctcccgacg aacatacagt gaggtttacc cttagccagc cattcgcacc gttcctctac
     9241 acgctggcga atgacggtgc atccattatc aatccggcgg tcttaaagga gcatgcggcg
     9301 gatgatgccc gtggtttcct cgcgcaaaat accgctggct ccggaccgtt tatgctgaaa
     9361 agctggcaaa aaggtcagca attagttctg gtgccaaatc cgcattaccc cggcaataaa
     9421 ccgaatttca aacgggtatc ggtaaaaatt atcggtgaaa gtgcttcccg tcgcctgcag
     9481 ctctcccgtg gcgacattga cattgccgat gcgctgccgg tggatcaact caacgcactg
     9541 aagcaggaaa acaaagtcaa tgtggcagag tatccgtcac tgcgcgtcac ctatctgtat
     9601 ctgaataaca gcaaagcgcc acttaatcag gcggatctgc gtcgggccat ttcctggtct
     9661 accgattatc agggtatggt taacggcatt ctgagtggta acggaaaaca aatgcgcggc
     9721 ccgattccgg aaggcatgtg gggatacgat gcgacggcaa tgcaatacaa ccatgacgaa
     9781 acgaaagcca aagccgaatg ggataaagtg acgagcaaac ccaccagcct gacgtttctc
     9841 tattctgata atgatccgaa ctgggagcct attgctctgg cgacacaatc cagtctcaac
     9901 aagctgggca tcaatgtgaa gctggaaaag ctggcgaacg ccaccatgcg cgacagagtg
     9961 ggtaaaggtg attacgacat cgcgattggc aactggagtc cggattttgc cgacccgtat
    10021 atgtttatga attactggtt tgagtccgac aaaaaaggtc tgccgggtaa ccgctcgttc
    10081 tatgaaaaca gtgaggtcga taagttactg cgcaatgcgc tagcgaccac cgaccagacg
    10141 cagcgtaccc gggactacca gcaggcacag aaaatcgtca ttgatgacgc tgcttatgtg
    10201 tatctgttcc agaaaaacta ccaactggcg atgaacaaag aggtgaaagg ctttgtgttc
    10261 aatcccatgc tggaacaggt cttcaatatc aataccatga gtaaataacg tgaccttctg
    10321 gagcatttta cgccaacgct gctgggggct ggtgctcgtg gtggcgggcg tctgcgtaat
    10381 tacgtttatt atctcgcatc ttatccccgg cgatccggcg cggttgctgg ctggcgaccg
    10441 cgccagcgat gccatcgtgg aaaatattcg ccagcaactg gggctggacc agccactgta
    10501 cgtacagttt taccgctacg tcagcgatct gtttcagggt gatctgggaa catccattcg
    10561 taccgggcgt ccggtgctgg aagagttgcg tatatttttc ccggcgacgc tggaactggc
    10621 tttttgtgcc ctgctgctgg cacttctgat tggcatcccg ctgggcatac tctctgcagt
    10681 ctggcgaaat cgctggctgg atcatctggt gcgaataatg gccattaccg gaatctccac
    10741 acctgcgttc tggcttgggc tgggcgtcat tgtgctgttt tatggacatc tgcaaattct
    10801 tcccggcggc ggaaggcttg atgactggct ggatccacca acgcacgtta ccggctttta
    10861 tctgctcgat gcgctgcttg aaggcaacgg tgaggtcttc ttcaatgcgt tgcaacatct
    10921 tatcttaccg gcattaacgc tggcgttcgt tcacctgggg attgtcgccc gccagatccg
    10981 ctcagcgatg ctggaacaat tgagtgaaga ctacattcgt accgcccggg ccagcggctt
    11041 gcccggctgg tatatcgttt tatgttatgc gctacccaat gcgttgatcc cctcgattac
    11101 cgtattggga ctggcactgg gcgatttgtt gtatggcgca gtgctcaccg aaaccgtttt
    11161 tgcctggccc ggaatgggtg catgggtagt aacatcaata caggcgctcg acttcccggc
    11221 agtgatgggc tttgccgtcg tggtttcgtt tgcttatgtg ctggtgaacc tggtggtgga
    11281 tttgctctat ttgtggattg atccgcgtat cggacgtgga ggtggtgaat gatgctaagc
    11341 gaggaaacgt cagccgtgcg cccaccaaaa caaacgcgat ttaacggtgc aaaactggta
    11401 tggatgctga aaggcagccc gctcaccgtg accggcgcag tcatcattgt attaatgcta
    11461 ttgatgatga ttttttcacc gtggctggcg acgcatgatc ccaacgccat tgatttaacc
    11521 gcccgccttt tgccgccttc tgcggcgcac tggtttggca ccgatgaagt gggacgcgat
    11581 ctgttcagcc gcgtactggt cggcagtcag caatcaattc tcgccggatt agtggtggtc
    11641 gccattgcag gtatgatggg gtcgctactc gggtgtctat ccggtgtgct tggcggacgc
    11701 gcagacgcga ttatcatgcg catcatggac attatgctgt cgattccttc gctggtactg
    11761 acaatggcac tggcagccgc tctcgggccg agtttgttta acgccatgct ggcgattgct
    11821 attgtacgaa ttccctttta tgtgcgcctg gcgcgggggc aaacattagt tgttcgccag
    11881 tatacctatg ttcaggcggc gaaaaccttt ggtgcgtcgc gttggcatct gatcaactgg
    11941 catattttac gtaactccct gccgccgctg attgtgcagg catcgctgga tatcggtagc
    12001 gcgattttaa tggccgccac gttgggattt attggccttg gtgctcaaca accgagtgct
    12061 gaatgggggg cgatggtggc gaatggtcgc aactatgtgc tcgatcaatg gtggtattgc
    12121 gcatttccgg gggcagcgat tttgcttacc gccgtcgggt ttaatctctt tggcgatggt
    12181 attcgcgatc tgcttgaccc gaaagcagga ggaaagcagt catgactcaa cccgttctgg
    12241 acattcaaca actgcatttg agtttccccg gttttaacgg cgacgttcac gcgctcaaca
    12301 atgtgtcatt gaaaatcaac cgcggtgaaa ttgtcggtct ggtgggagaa tccggctcgg
    12361 gtaaatcagt cactgcaatg ttgattatgc gtctgctacc gacgggcagt tattgcgtac
    12421 atcggggaca tatttcactg ctgggagaag atgttcttaa cgctcgggaa aagcagcttc
    12481 gtcagtggcg cggcgcacga gtggcgatga tctttcagga accgatgacc gccctcaatc
    12541 cgacacgtcg aataggtctt cagatgatgg acgtgatccg ccatcatcaa ccaataagtc
    12601 gtcgggaagc cagagctaaa gcgattgccc tgctggaaga gatgcaaatc ccggatgccg
    12661 tggaagttat gtcgcgctat ccgtttgagc tttcaggtgg tatgcgccag cgggtaatga
    12721 ttgcgctggc attctcctgc gagccgcaat tgattattgc cgacgaaccg actacggcgc
    12781 tggacgtcac ggtacagttg caggtactgc gtctgcttaa acataaagcc cgcgccagtg
    12841 gaactgcggt actgttcatc agccatgata tggccgtggt gtcgcaactg tgcgatagcg
    12901 tttacgtgat gtatgccgga agcgtgattg agagcggcgt gacggcagac gttatccatc
    12961 atccccggca tccgtatacc attggtttgc tgcaatgcgc accggaacat ggaataccgc
    13021 gccagccatt acccgctatt ccggggacgg taccaaacct cacccatttg cctgacggct
    13081 gcgcttttcg cgatcgttgc tatgcggcag gtacacagtg tgaaaacgtc ccggcgctga
    13141 cagcgtgtgg tgacaacaac caccgctgcg cctgttggta tccgcagcag gaggtcatta
    13201 gtgtctgata cgttattaac gttacgcgac gtccatatca atttccctgc ccgaaaaaac
    13261 tggcttggta aaactacgga acatgttcat gccatcaatg gtattgattt acagatccgt
    13321 cgtggtgaaa ccttagggat cgtcggtgag tcaggctgcg gcaaaagcac cctcgcacag
    13381 cttttaatgg gtatgctgca accgagccac gggcagtaca tccgttcagg atcacaacgc
    13441 attatgcaga tggtgtttca ggacccgctc tcttcgctta atccgcgctt accggtgtgg
    13501 cgcatcatca cggaaccgct ctggattgct aagcgtagta gtgaacaaca gcggcgagcg
    13561 ttggcagagg agctggctgt gcaggtgggt attcgtccgg agtatctcga tcgcttgcct
    13621 catgcgttct caggcgggca gcggcaacgt atcgccattg ccagagcact ctcttcgcag
    13681 cctgacgtga ttgtgcttga tgaaccaacc tcggcgctgg atatctccgt gcaggcgcag
    13741 atcctcaatt tactggtaac gctacaggaa aatcgcgagc tgacttatgt gctgatttca
    13801 cacaatgtct ccgtgatacg tcatatgagc gatcgggtgg cggtgatgta tctcggacag
    13861 attgtggaat taggagacgc gcaacaggtg ctgacagctc cggcacatcc atacacccga
    13921 ttattgctgg attccctccc cgccattgat aaaccgctgg aggaagaatg ggcattacgt
    13981 aaaacggatc taccaggaaa ccgcacgttg ccacaaggct gttttttctg cgaacgttgc
    14041 cctctggcaa cccacgggtg tgaagtccgg caatcattaa caataaggga ggacggacgt
    14101 gagatccggt gctggcgggc gctgtagaga cgctaacaac gcatcaggca atcacgcgcc
    14161 tgacgcgtca tacggcaacg ctttatgatt tcaactggta atcaagcgta atttcagctt
    14221 tcagtacctg agaaaccggg catcctgctt ttgctttctg gataatgccg tcaaaagtag
    14281 aggcatcaat acccggcacc gcaacttcgc ttttcagtgc gattttagta atcgcaaaac
    14341 cggcatccac tttatccagc gacacatcgg cggtggtatc aatcgatgtt ggcgtgaatc
    14401 ccgcttcccc cagcattaat gaaagcgcca ttgagaaaca tgcggcatgc gctgcgccaa
    14461 tcagttcttc agggttggtt cctttttcgc cttcaaaacg cgtgttaaat ccatacggct
    14521 gttggttcag cacgccgctc tcggtggata cagttccctt cccgcgtttg atatcgcctt
    14581 cccagtgtgc ctgacctttc ttatggattg tcattgttgc tctcctgtgg gcttaaaaag
    14641 gaaattatag actacgtgag aaaccgcacg aggataagca ggttattccg aataaaatcc
    14701 ggcgaaaata ttacttctgc acttaaatta aattcccaca atagattacc gggaatatat
    14761 atatcaagag atattgcatc aatatcattc aactcaccac aataataata cctatcaata
    14821 tcatggggtt aaaatataaa aattatcaac caggactaat cttaacaacg aagcggcaaa
    14881 tatttgccat gctgaatgtg ctttataaat ctgcgatccg tagcagacac cataaataca
    14941 cagacacgga gaatcactat gtttacttat tatcaggcag aaaattcaac agcagaaccc
    15001 gctctggtta atgccattga gcaaggcctt cgggcagagc acggtgttgt cactgaagat
    15061 gacattctca tggagctgac caaatgggtg gaagcttctg ataacgacat cctcagtgat
    15121 atctaccagc aaacgatcaa ttatgtggtc agtggccagc accctacact ttaaggtgct
    15181 atgcttgatc ggcaacctaa tttaggggtt tagcacgtgt ttcttcgcta cggcgatgtt
    15241 gtccttaaaa ctagcaacag gattgaggag ttaaaatgaa atcgaaccgt caggcacgtc
    15301 atattcttgg actggaccat aaaatatcta accagcgcaa aatagttact gaaggtgaca
    15361 aatccagcgt ggtaaataac ccaaccggca gaaaacgccc cgctgaaaag taattcataa
    15421 ccatcagtcc tcaatgacga ttaaacacca ttgcctgcgc aatggtgttt ttgtttttat
    15481 ctgctttata cttggggccg acgccctggc ggtaaagcaa agacgataaa agcgtgccag
    15541 ggatggatat tcaaaaaaga gtgagtgaca tggaaccaaa aacaaaaaaa cagcgttcgc
    15601 tttatatccc ttacgctggc cctgtattgc tggaatttcc gttgttgaat aaaggcagcg
    15661 ccttcagcat ggaagaacgc cgtaacttca acctgctggg gttactgccg gaagtggtcg
    15721 aaaccatcga agaacaagcg gaacgagcat ggatccagta tcagggattc aaaaccgaaa
    15781 tcgacaaaca catctacctg cgtaacatcc aggacactaa cgaaaccctc ttctaccgtc
    15841 tggtaaacaa tcatcttgat gagatgatgc cagtcattta caccccaacc gtcggcgcag
    15901 cctgtgagcg tttttctgag atctaccgcc gttcacgcgg tgtgtttatc tcttaccaga
    15961 accgccacaa tatggacgat attctgcaaa acgtaccaaa ccataatatc aaagtgattg
    16021 tggtgactga cggcgaacgt attctggggc ttggtgacca gggcatcgga gggatgggca
    16081 ttccgatcgg taaactgtca ctctataccg cctgtggcgg catcagcccg gcgtataccc
    16141 ttccggtggt gctggatgtc gggacgaaca accaacagct gcttaacgat ccgctgtata
    16201 tgggctggcg taatccgcgt atcactgacg acgaatacta tgaattcgtt gatgaattta
    16261 tccaggctgt gaaacaacgc tggccggacg tactgttgca gtttgaagac ttcgcacaaa
    16321 aaaatgcgat gccgttactt aaccgctatc gcaatgaaat ttgttctttt aacgatgaca
    16381 ttcagggcac cgcggcggta acggtcggca cactgatcgc agccagccgc gcggcgggtg
    16441 gtcaactgag cgagaaaaag attgtcttcc tcggcgcagg ctctgcgggg tgcgggattg
    16501 ccgaaatgat cattgcccag actcagcgtg aaggattaag cgaggaaacg gcgcggcaga
    16561 aagtctttat ggtcgatcgc tttggcctgc tgaccgacaa gatgccgaac ctgctgcctt
    16621 tccagaccaa actggtgcag aaacgcgaaa acctcagtga ctgggatacc gacagcgatg
    16681 tgctgtcact gctggatgtg gtgcgcaatg taaaaccaga tattctgatt ggcgtctcag
    16741 gacagaccgg gctgtttacg gaagagatca tccgtgagat gcataaacac tgtccgcgtc
    16801 cgatcgtgat gccgctgtct aacccgactt cacgcgtcga agcaacaccg caggacatta
    16861 tcgcctggac tgaaggtaac gcgctggtcg ccactggcag tccatttaat ccagtggtat
    16921 ggaaagataa aatctaccct atcgcccagt gtaataacgc ctttattttc ccgggcatcg
    16981 ggctgggtgt tattgcttcc ggcgcgtcac gtatcaccga tgaaatgctg atgtcggcaa
    17041 gtgaaacgct tgctcagtat tcgccgctgg tcctgaacgg cgaaggtctg gtactaccgg
    17101 aactgaaaga tatacagaaa gtctcccgcg caattgcgtt tgcggttggc aaaatggcgc
    17161 agcagcaagg cgtggcggtg aaaacctccg ccgaagctct gcaacaggcc attgatgata
    17221 atttctggca agccgaatac cgcgactacc gccgtacctc catctaagcc tgcgcccggt
    17281 agtgaaggct accgggcaat ttccctctcc ctttttcaga tctcatccat actgggtagt
    17341 ggcgaataaa tctcatttgc ctcacctgct atgcagaaca tcatccgaaa aggaggaact
    17401 atgaaggctg cagttgttac gaaggatcat catgttgacg ttacggataa aacactgcgc
    17461 tcactgaaac atggcgaagc cctgctgaaa atggagtgtt gtggtgtatg tcataccgat
    17521 cttcatgtta agaatggcga ttttggtgac aaaaccggcg tgattctggg acacgaaggt
    17581 attggtgtgg tggcagaagt tggcccaggt gtcacttcat taaaaccagg cgatcgtgcc
    17641 agcgtggcgt ggttctatga aggatgcggt cattgcgaat actgtaacag tggtaacgaa
    17701 acgctctgcc gttcagtaaa aaatgccgga tacagcgttg atggcgggat ggctgaagaa
    17761 tgcatcgtgg tcgccgatta cgcggttaaa gtgccggatg gcctggactc ggcggcggcc
    17821 agcagcatta cctgtgcggg ggtcaccacc tacaaagccg ttaagctgtc aaaaattcgt
    17881 ccagggcagt ggattgctat ctacggtctt ggcggtctgg gtaacctcgc cctgcaatac
    17941 gcgaagaatg tctttaacgc caaagtgatc gccattgatg tcaatgatga gcagttaaaa
    18001 ctggcaaccg aaatgggcgc tgatttagcg attaactcac gcaccgaaga cgccgccaaa
    18061 attgtgcagg aaaaaaccgg tggcgctcac gctgcggtgg tgacagcggt agctaaagct
    18121 gcgtttaact cggcagttga tgctgtccgt gcaggcgggc gtgttgtggc agtcggtctg
    18181 ccgccggagt ctatgagcct ggatatccca cgccttgtgc tggatggtat tgaagtggtc
    18241 ggttcgctgg tcggcacgcg ccaggattta actgaagcct tccagtttgc cgccgaaggc
    18301 aaagtggtac cgaaggtcgc cctgcgtccg ttagcggaca tc
//
LOCUS       DAMZFT010000095        18317 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.095, whole genome shotgun sequence.
ACCESSION   DAMZFT010000095 DAMZFT010000000
VERSION     DAMZFT010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18317)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18317)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18317
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.095"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(<1..271)
                     /locus_tag="PN977_003437"
     CDS             complement(<1..271)
                     /locus_tag="PN977_003437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HDI6043440.1"
                     /translation="MIEWIIRRSVANRFLVLMGALFLSIWGTWTIINTPVDALPDLSD
                     VQVIIKTSYPGQAPQIVENQVTYPLTTTMLSVPGAKTVRGFSQFGD"
     gene            complement(283..1506)
                     /gene="cusB"
                     /locus_tag="PN977_003438"
     CDS             complement(283..1506)
                     /gene="cusB"
                     /locus_tag="PN977_003438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     adaptor subunit CusB"
                     /protein_id="HDI6043441.1"
                     /translation="MKKIALIIGSMIAGGIISAAGFTWFAKAEPPAEKTPTAERKILF
                     WYDPMYPNTRFDKPGKSPFMDMDLVPKYADEESSASGVRIDPTQTQNLGVKTATVTRG
                     PLTFAQSFPANVSYNEYQYAIVQARAAGFIDKVYPLTVGDKVQKGTPLLDLTIPDWVE
                     AQSEYLLLRETGGTATQTEGILERLRLAGMPEADIRRLIATQKIQTRFTLKAPIDGVI
                     TAFDLRAGMNIAKDNVVAKIQGMDPVWVTAAIPESIAWLVKDASQFTLTVPARPDKTL
                     TIRKWTLLPGVDAATRTLQLRLEVDNADEALKPGMNAWLQLNTASEPMLLIPSQALID
                     TGNEQRVITVDADGRFVPKRVAVFQASQGVTALRSGLAEGEKVVSSGLFLIDSEANIS
                     GALERMRSESATHAH"
     gene            complement(1522..1854)
                     /gene="cusF"
                     /locus_tag="PN977_003439"
     CDS             complement(1522..1854)
                     /gene="cusF"
                     /locus_tag="PN977_003439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     metallochaperone CusF"
                     /protein_id="HDI6043442.1"
                     /translation="MKKALQVAMFSLFTVIGFNAQANEHHHESMSEAQPQVISATGVV
                     KGVDLESKKITIHHDPIAAVNWPEMTMRFTITPQTKMSEIKTGDKVAFNFVQQGNLSL
                     LQDIKVSQ"
     gene            complement(1878..3260)
                     /gene="cusC"
                     /locus_tag="PN977_003440"
     CDS             complement(1878..3260)
                     /gene="cusC"
                     /locus_tag="PN977_003440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter outer
                     membrane channel CusC"
                     /protein_id="HDI6043443.1"
                     /translation="MSPCKLLPFCVALALTGCSLAPDYQRPAMPVPQQFSLSQNGLVN
                     AADNYQNAGWRTFFVDNQVKTLISEALVNNRDLRMAALKVQEARAQYRLTDADRYPQL
                     NGEGSGSWSGNLKGNTATTREFSTGLNASFDLDFFGRLKNMSEAERQNYLATEEAQRA
                     VHILLVSNVAQSYFNQQLAYAQLQIAEETLRNYQQSYAFVEKQLLTGSSNVLALEQAR
                     GVIESTRSDIAKRQGELAQANNALQLLLGSYGKLPQAQTVNSDSLQSVKLPAGLPSQI
                     LLQRPDIMEAEHALMAANANIGAARAAFFPSISLTSGISTASSDLSSLFNASSGMWNF
                     IPKIEIPIFNAGRNQANLDIAEIRQQQSVVNYEQKIQNAFKEVADALALRQSLNDQIS
                     AQQRYLASLKITLQRARALYQHGAVSYLEVLDAERSLFATRQTLLDLNYARQVNEISL
                     YTALGGGWQQ"
     gene            3417..4100
                     /gene="cusR"
                     /locus_tag="PN977_003441"
     CDS             3417..4100
                     /gene="cusR"
                     /locus_tag="PN977_003441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper response regulator transcription factor
                     CusR"
                     /protein_id="HDI6043444.1"
                     /translation="MKLLIVEDEKKTGEYLTKGLTEAGFVVDLADNGLNGYHLAMTGD
                     YDLIILDIMLPDVNGWDIVRMLRSANKGMPILLLTALGTIEHRVKGLELGADDYLVKP
                     FAFAELLARVRTLLRRGAAVIIESQFQVADLMVDLVSRKVTRSGTRITLTSKEFTLLE
                     FFLRHQGEVLPRSLIASQVWDMNFDSDTNAIDVAVKRLRGKIDNDFEPKLIQTVRGVG
                     YMLEVPDGQ"
     gene            4090..5532
                     /gene="cusS"
                     /locus_tag="PN977_003442"
     CDS             4090..5532
                     /gene="cusS"
                     /locus_tag="PN977_003442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) sensor histidine kinase CusS"
                     /protein_id="HDI6043445.1"
                     /translation="MVSKPFQRPFSLATRLTFFISLATIAAFFAFAWIMIHSVKVHFA
                     EQDINDLKEISATLERVLNHPDETQARRLMTLEDIVSGYSNVLISLVDSHGKTVYHSP
                     GAPDIREFARDAIPDKDARGGEVYLLSGPTMMMPGHGHGHMEHSNWRMINLPVGPLVD
                     GKPIYTLYIALSIDFHLHYINDLMNKLIMTASVISILIVFIVLLAVHKGHAPIRSVSR
                     QIQNITSKDLDVRLDPQTVPIELEQLVLSFNHMIERIEDVFTRQSNFSADIAHEIRTP
                     ITNLITQTEIALSQSRSQKELEDVLYSNLEELTRMAKMVSDMLFLAQADNNQLIPEKK
                     MLNLADEVGKVFDFFEALAEDRGVELRFVGDECQVAGDPLMLRRALSNLLSNALRYTP
                     TGETIVVRCQTVDHLVQVTVENPGTPIAPEHLPRLFDRFYRIDPSRQRKGEGSGIGLA
                     IVKSIVVAHKGTVAVTSDARGTRFVIILPA"
     gene            5682..7919
                     /gene="nrfB"
                     /locus_tag="PN977_003443"
     CDS             5682..7919
                     /gene="nrfB"
                     /locus_tag="PN977_003443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage adsorption protein NrfB"
                     /protein_id="HDI6043446.1"
                     /translation="MDWLLDVFATWLYGLKVIAITLAVIMFISGLDDFFIDVVYWVRR
                     IKRKLSVYRRYPRMSYRELYKPDEKPLAIMVPAWNETGVIGNMAELAATTLDYENYHI
                     FVGTYPNDPDTQRDVDEVCARFPNVHKVVCARPGPTSKADCLNNVLDAITQFERSANF
                     AFAGFILHDAEDVISPMELRLFNYLVERKDLIQIPVYPFEREWTHFTSMTYIDEFSEL
                     HGKDVPVREALAGQVPSAGVGTCFSRRAVTALLADGDGIAFDVQSLTEDYDIGFRLKE
                     KGMTEIFVRFPVVDEAKEREQRKFLQHARTSNMICVREYFPDTFSTAVRQKSRWIIGI
                     VFQGFKTHKWTSSLTLNYFLWRDRKGAISNFVSFLAMLVMIQLLLLLAYESLWPDAWH
                     FLSIFSGSAWLMTLLWLNFGLMVNRIVQRVIFVTGYYGLTQGLLSVLRLFWGNLINFM
                     ANWRALKQVLQHGDPRRVAWDKTTHDFPSVTGDTRSLRPLGQILLENQVITEEQLDTA
                     LRNRVEGLRLGGSMLMQGLISAEQLAQALAEQNGVAWESIDAWQIPSSLIAEMPASVA
                     LHYAVLPLRLDNDELIVGSEDGIDPVSLAALTRKVGRKVRYVIVLRGQIVTGLRHWYA
                     RRRGHDPRAMLYNAVQHQWLTEQQAGEIWRQYVPHQFLFAEILTTLGHINRSAINVLL
                     LRHERSSLPLGKFLVTEGVISQETLDRVLTIQRELQVSMQSLLLKAGLNTEKVAQLES
                     ENEGE"
     gene            7906..10878
                     /gene="nfrA"
                     /locus_tag="PN977_003444"
     CDS             7906..10878
                     /gene="nfrA"
                     /locus_tag="PN977_003444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage adsorption protein NfrA"
                     /protein_id="HDI6043447.1"
                     /translation="MKENNLNRVIGWSGLLLTSLLSTSALADNIGTSAEELGLSDYRH
                     FVIYPRLDKALKAQKNNDEATAIREFEYIHQQVPDNIPLTLYLAEAYRHFGHDDRARL
                     LLEDQLKRHPGDARLERSLAAIPVEVKSVTTVEELLAQQKACDAAPTLRCRSEVGQNA
                     LRLAQLPVARAQLNDATFAASPEGKTLRTDLLQRAIYLKQWSQADTLYNEARQQNTLS
                     AAERRQWFDVLLAGQLDDRILALQSQGIFTDPQSYITYATALAYRDEKARLQRYLIEN
                     KPLFTTDAQEKSWLYLLSKYSANPVPALANYTVQFADNRQYVVGATLPVLLKEGQYDA
                     AQKLLATLPANEMLEERYTVSVATHNKAEALRLARLLYQQEPANLTRLDQLTWQLMQN
                     EQSREAADLLLQRYPFQGDARVSQTLMARLASLLESHPYLATPAKVAILSKPLPLAEQ
                     RQWQSQLPGIADNCPAIVRLLGDMSPSYDAAAWNRLAKCYRDTLPGVALYAWLQAEQR
                     QPNAWQHRAVAYQAYQVEDYATALAAWQKISLHDMSNEDLLAAANTAQAAGNGATRDR
                     WLQQAEQRGLGNNALYWWLHAQRYIPGQPELALNDLTRSINIAPSANAYVARATIYRQ
                     RHNVPAAVSDLRAALELEPNNSNTQAALGYALWDSGDIAQSREMLEQAHKGLPDDPAL
                     IRQLAYVNQRLDDMPATQHYARLVIDDIDNQALITPLTPEQNQQRFNFRRLHEEVGRR
                     WTFSFDSSIGLRSGAMSTANNNVGGAAPGKSYRSYGQLEAEYRIGRNMLLEGDLLSVY
                     SRVFADTGENGVMMPVKNPMSGTGLRWKPLRDQIFFLAVEQQLPLNGQNGASDTMLRA
                     SASFFNGGKYSDEWHPNGSGWFAQNLYLDAAQYIRQDIQAWTADYRVSWHQKVANGQT
                     IEPYAHVQDNGYRDKGTQGAQLGGVGVRWNIWTGETHYDAWPHKVSLGVEYQHTFKAI
                     NQRNGERNNAFLTIGVHW"
     gene            10879..11769
                     /locus_tag="PN977_003445"
     CDS             10879..11769
                     /locus_tag="PN977_003445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4434 family protein"
                     /protein_id="HDI6043448.1"
                     /translation="MRKFIFVLLTLLLVSPFSFAMKGIIWQPQNRDSQVTDTQWQGLM
                     SQLRLQGFDTLVLQWTRYGDAFTQPEQRALLFKRAAAAQQAGLKLIVGLNADPEFFMH
                     QKQSSAALESYLNRLLAADLQQARLWSAVPGVTPDGWYISAEIDDLNWRSEAARQPLL
                     TWLNNAQRLISDVSAKPVYISSFFAGNMSPDGYRQLLEHVKATGVNVWVQDGSGVDKL
                     TAEQRERYLQASADCQSSAPASGIVYELFVAGKGKTFTAKPKPDAEIASLLAKRSSCG
                     KDTLYFSLRYLPVAHGILEY"
     gene            11952..12713
                     /gene="envY"
                     /locus_tag="PN977_003446"
     CDS             11952..12713
                     /gene="envY"
                     /locus_tag="PN977_003446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator EnvY"
                     /protein_id="HDI6043449.1"
                     /translation="MQLSSSEPCVVILTEKEVEVSVNNHATFTLPKNYLAAFACNNNV
                     IELSTLNHVLITHINRNIINDYLLFLNKNLTCIKPWSRLATPVIACHSRTPEVFRLAA
                     NHSKQQPSKPCEAELTRALLFTVLSNFLEQSRFIALLMYILRSSVRDSVCRIIQSDIQ
                     HYWNLRIVASSLCLSPSLLKKKLKNENTSYSQIVTECRMRYAVQMLLMDNKNITQVAQ
                     LCGYSSTSYFISVFKAFYGLTPLNYLAKQRQKVMW"
     gene            complement(12759..12835)
                     /locus_tag="PN977_003447"
     tRNA            complement(12759..12835)
                     /locus_tag="PN977_003447"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12799..12801),aa:Arg,seq:tct)
     gene            13077..13709
                     /gene="fimZ"
                     /locus_tag="PN977_003448"
     CDS             13077..13709
                     /gene="fimZ"
                     /locus_tag="PN977_003448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria biosynthesis transcriptional regulator
                     FimZ"
                     /protein_id="HDI6043450.1"
                     /translation="MKPTSVIIMDTHPIIRMSIEVLLQKNNELQIVLKTDDYRITIDY
                     LRTRPVDLIIMDIDLPGTDGFTFLKRIKQIQSTVKVLFLSSKSECFYAGRAIQAGANG
                     FVSKCNDQNDIFHAVQMILSGYTFFPSETLNYIKSNKCSTNSSTVTVLSNREVTILRY
                     LVSGLSNKEIADKLLLSNKTVSAHKSNIYGKLGLHSIVELIDYAKLYELI"
     gene            complement(13712..14227)
                     /gene="sfmF"
                     /locus_tag="PN977_003449"
     CDS             complement(13712..14227)
                     /gene="sfmF"
                     /locus_tag="PN977_003449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria assembly protein"
                     /protein_id="HDI6043451.1"
                     /translation="MRRVLFSCFCGLLWSSSGWAVDPLGTININLHGNVVDFSCTVNT
                     ADKDKTVDLGRWPTTQLLSAGDTTALVPFSLRLEGCPPGSVAILFTGTPASDANLLAL
                     DDPAMAQTVAIELRNSDRSRLALGEASSTEEVDANGNVTLNFFANYRALASGVQPGVA
                     KADAIFMINYN"
     gene            complement(14238..15245)
                     /gene="fimH"
                     /locus_tag="PN977_003450"
     CDS             complement(14238..15245)
                     /gene="fimH"
                     /locus_tag="PN977_003450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrin D-mannose specific adhesin FimH"
                     /protein_id="HDI6043452.1"
                     /translation="MKIICRLLLAMACLCLANISWATVCANSTGVAEDEHYDLSNVFN
                     STNNQPGQIVVLPEKSGWVGVSAICPPGTLVNYTYRSYVTNFIVQETIDNYKYMQLND
                     YLLGAMSLVDSVMDIQFPPQNYIRMGTDPNVSQNLPFGVMDSRLIFRLKVIRPFINMV
                     EIPRQVMFTVYVTSTPNDPLVTPVYTISFGGRVEVPQNCELNAGQIVEFDFGDIGASL
                     FSAAGPGNRPAGVMPQTKSIAIKCTNVAAQAYLTMRLEASAVSGQAMVSDNQDLGFIV
                     ADQNDTPITPNDLNSVIPFRLDAAAAANVTLRAWPISITGQKPTEGPFSALGYLRVDY
                     Q"
     gene            complement(15258..17867)
                     /locus_tag="PN977_003451"
     CDS             complement(15258..17867)
                     /locus_tag="PN977_003451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis usher protein"
                     /protein_id="HDI6043453.1"
                     /translation="MKIPTTTDIPQRYTWCLAGICYSSLAILPSFLSYAESYFNPAFL
                     LENGTSVADLSRFERGNHQPAGVYRVDLWRNDEFIGSQDIVFESTTVNTGDKSGGLMP
                     CFNQALLERIGLNSSAFPELAQQQNNKCINLLKAVPDATINFDFAAMRLNITIPQIAL
                     LSSAHGYIPPEEWDEGIPALLLNYNFTGNRGNGNDSYFFSELSGINIGPWRLRNNGSW
                     NYFRGNGYHSEQWNNIGTWVQRAIIPLKSELVMGDGNTGSDIFDGVGFRGVRLYSSDN
                     MYPDSQQGFAPTVRGIARTAAQLTIRQNGFIIYQSYVSPGAFEITDLHPTSSNGDLDV
                     TIDERDGNQQNYTIPYSTVPILQREGRFKFDLTAGDFRSGNSQQSSPFFFQGTALGGL
                     PQEFTAYGGTQLSANYTAFLLGLGRNLGNWGAVSLDVTHARSQLADDSRHEGDSIRFL
                     YAKSMNTFGTNFQLMGYRYSTQGFYTLDDVAYRRMEGYEYDYDYDGEHRDEPIIVNYH
                     NLRFSRKDRLQLNISQSLNDFGSLYISGTHQKYWNTSDSDTWYQVGYTSSWVGISYSL
                     SFSWNESVGIPDNERIVGLNVSVPFNVLTKRRYTRENALDRAYASFNANRNSNGQNNW
                     LAGVGGTLLEGHNLSYHVSQGDTSNNGYTGSATANWQAAYGTLGVGYNYDRDQHDVNW
                     QLSGGVVGHENGITLSQPLGDTNVLIKAPGAGGVRIENQTGILTDWRGYAVMPYATVY
                     RYNRIALDTNTMGNSIDVEKNISSVVPTQGALVRANFDTRIGVRALITVTQGGKPVPF
                     GSPVRENSTGITSMVGDDGQVYLSGAPLSGELLVQWGDGANSRCIAHYVLPKQSLQQA
                     VTVISAVCTHPGS"
     gene            complement(17898..>18317)
                     /locus_tag="PN977_003452"
     CDS             complement(17898..>18317)
                     /locus_tag="PN977_003452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HDI6043454.1"
                     /translation="IIYTGPPLAADRESLFWMNVKTIPSVDKNALNGRNVLQLAILSR
                     MKLFLRPIQLQELPAEAPDTLKFSRSGNYINVHNPSPFYVTLVNLQVGSQKLGNAMAA
                     PRVNSQIPLPSGVQGKLKFQTVNDYGSVTPVREVNLN"
BASE COUNT         4599 a         4685 c         4516 g         4517 t
ORIGIN      
        1 agtcgccaaa ctgcgagaaa ccgcgcactg tcttcgcgcc aggtaccgac aacatggtgg
       61 tggttagcgg ataagtcacc tgattttcaa cgatttgcgg tgcctgaccg ggatagctgg
      121 ttttaataat cacctgtaca tcagagagat ccggcagcgc atccactggc gtgttaatga
      181 tggtccaggt gccccagatg ctcagaaaca acgcgcccat cagcaccaga aaacggttcg
      241 ccaccgagcg acgaataatc cattcaatca ttggttattc cctcaatgcg catgggtagc
      301 actttcagag cgcatccgct ccagtgcgcc agaaatattg gcttcagaat caatcaggaa
      361 caggccgctg gaaaccacct tttcaccttc cgccagacca gagcgtaacg cggtgacgcc
      421 ttgcgatgcc tggaaaacgg caacgcgttt cggtacaaag cgcccgtcag catcaacggt
      481 aatcacccgc tgttcgttgc cggtatcaat cagcgcctgt gacggaatga gcagcatcgg
      541 ttcgctggcg gtattgagtt gcaaccaggc gttcatgccc ggttttagcg cctcgtcggc
      601 gttgtcgact tccagacgca gttgtagcgt gcgggtcgcg gcatccacgc cgggtagcag
      661 ggtccatttg cgaatggtga gtgttttatc cggtcgcgcc ggaacggtga gggtaaactg
      721 cgaggcatct ttcaccagcc aggcgatgga ctccgggatc gcagcagtga cccacaccgg
      781 gtccataccc tgaattttcg cgaccacgtt atctttggcg atattcattc ccgcgcgcag
      841 atcaaacgcg gtgatcacgc catcaatggg cgctttgagc gtaaagcgag tctggatttt
      901 ttgcgtggcg atcaggcggc gaatatccgc ctccggcatt cccgccagtc tcagccgctc
      961 aagaatgcct tcagtctggg tcgccgtacc gccggtttcg cgcagcagta aatactcact
     1021 ctgcgcttcc acccagtcgg gaatggtcag gtcgagaagc ggtgtgccct tttgtacttt
     1081 atcgcccacg gtaagcggat acaccttgtc gataaacccg gcagcgcgag cctgcacaat
     1141 ggcatactga tactcgttgt aactgacatt cgccgggaaa ctctgggcaa aagtcagcgg
     1201 cccgcgcgtg acggtcgccg ttttcacccc cagattctgc gtctgggtcg ggtcaatgcg
     1261 tacaccagac gcagaactct cttcatcggc atatttcggc accagatcca tatccataaa
     1321 cggcgattta cctggtttat cgaaccgcgt attgggatac atcgggtcgt accagaataa
     1381 gattttacgt tctgcggtcg gcgttttttc tgcgggcggt tcagcctttg caaaccaggt
     1441 aaaacctgcc gcagaaataa taccgcccgc gatcatgctg ccgataataa gcgcgatttt
     1501 tttcatctca ttaaacctgg gttactggct gactttaata tcctgtaata aagaaaggtt
     1561 gccctgctgg acaaaattaa acgccacttt gtcgccggtt ttaatttcgc tcattttcgt
     1621 ctgcggggtg atggtaaagc gcatggtcat ctccggccag ttcacggcag caatcggatc
     1681 gtgatggatg gtgatttttt tgctttccag atcaacacct tttaccacgc cagtggcgct
     1741 aataacctgt ggctgtgctt cgctcatgga ttcatgatga tgttcgttag cctgggcatt
     1801 aaagccaata acggtaaaca gactaaacat tgcgacttgc agtgcttttt tcatttattc
     1861 tctcctggag ttaaaaatta ttgctgccaa ccgccgccta aggcggtata taaagaaatt
     1921 tcgttaacct gacgggcata attcaggtca agtaaagttt gtcgggttgc aaataaagaa
     1981 cgctcggcat ccagcacttc cagataactt actgcgccgt gctgatataa tgcccgcgcc
     2041 cgttgcaaag taattttcag cgatgccaga taacgctgct gggcgctgat ttgatcgttc
     2101 aggctttgac gtaatgccag cgcatctgcc acttctttaa aggcattctg gattttctgt
     2161 tcataattca ccaccgactg ctgctggcga atttcggcga tatccagatt ggcctggttg
     2221 cgtccggcat taaaaatggg gatctcaatt ttgggtataa aattccacat cccgctgctg
     2281 gcgttaaata atgacgacag atcgctactg gcggtcgata ttccgctggt caggctgata
     2341 gacgggaaaa atgccgcgcg tgcagcgcca atattggcat tagccgccat taacgcgtgt
     2401 tcagcttcca taatatcagg gcgctgcaat aagatttgcg acggcaagcc cgccggtaat
     2461 ttaacgcttt gcaggctgtc gctgtttact gtctgcgctt gcggcagctt gccgtagctt
     2521 cccaataaca gttgcaatgc attattcgcc tgcgccagtt ccccctgacg tttagcgatg
     2581 tcgctgcggg tactttctat caccccgcga gcctgttcta gcgccagaac attgctgcta
     2641 ccggtcaaca gttgtttttc gacaaatgca tatgactgct gataattacg cagcgtttct
     2701 tcggctattt gcaattgcgc atacgccagt tgctgattga aatagctttg cgcgacatta
     2761 gaaaccagca gaatatgcac cgcgcgctga gcttcctcag tagctaaata attttgacgc
     2821 tcggcttcgc tcatgttctt taagcgcccg aaaaaatcga ggtcaaagct ggcgttaagg
     2881 ccggtcgaaa actcccgtgt cgtggcggta ttccctttaa gattaccgct ccagctgccg
     2941 ctgccctcgc cattgagctg tgggtagcgg tcggcatcgg tcagacgata ttgcgcccgt
     3001 gcctcctgca ctttcagcgc cgccatgcgc aaatcccggt tattcaccaa cgcctcgcta
     3061 atcagcgtct tcacctgatt atcaacaaaa aaggtgcgcc agcccgcgtt ctggtagtta
     3121 tctgctgcgt taaccaggcc gttctggctg agtgagaact gctgcggcac gggcattgcc
     3181 ggacgctgat aatccggtgc cagtgaacaa ccggttagcg caagggccac acaaaatggc
     3241 agaagtttac aaggagacat aggctcataa tttctggtga ttttatgccg ccaactttac
     3301 tcgccaggct ctgattttcc ggtgacagga aaatgacaaa attgtcattt tgccaataag
     3361 cgattgccat ctgatcccgc tactctagaa ttgcccgggc aacatgcgga ggaaatatga
     3421 aactgttgat tgtcgaagat gaaaagaaaa ccggagaata cttgaccaaa gggttaaccg
     3481 aagccggttt tgtggtcgat ttggccgaca acgggctgaa tggctaccat ctggcgatga
     3541 ccggtgatta tgatctgata atcctcgata ttatgctgcc ggacgtgaac ggctgggata
     3601 tcgtgcgcat gttgcgttcc gccaataagg ggatgccgat tctgttgctt accgcgcttg
     3661 gcaccattga acatcgcgtc aaggggctgg agttgggggc ggatgactat ctggttaagc
     3721 cgttcgcttt tgctgaactg ctggcgcggg tgcgcactct actgcggcgc ggggcggcgg
     3781 tgattatcga aagtcagttt caggttgccg atctgatggt cgatctcgtc agccgcaaag
     3841 tcacccgtag cggcacgcgc atcacgttga ccagtaaaga atttactttg ctggagtttt
     3901 tcctccgcca tcagggtgaa gtgctgcccc gctcgcttat cgcctcgcag gtgtgggaca
     3961 tgaatttcga cagcgacact aacgccattg atgtggcggt gaagcggcta cgcggcaaaa
     4021 tcgacaacga ctttgagccg aagctgattc agaccgtgcg cggcgtgggc tatatgcttg
     4081 aggtgccgga tggtcagtaa gccatttcag cgcccgtttt cgctggcaac ccgtctgacc
     4141 ttttttatca gcctggccac catcgcggcg tttttcgcct ttgcatggat catgatccac
     4201 tcggtaaaag tgcattttgc cgagcaggat attaatgatt taaaagagat tagcgccacc
     4261 cttgaacggg tacttaatca ccctgacgaa acgcaagccc gacgcttaat gacgctggaa
     4321 gatatcgtca gtggttattc caacgtgttg atttctctgg tagatagcca cggtaaaacg
     4381 gtgtatcact cccccggtgc gccggatatc cgcgagtttg cacgtgatgc catacccgat
     4441 aaagacgctc ggggcggcga ggtgtatctc ctttccggcc cgacaatgat gatgccaggc
     4501 cacggtcacg gacatatgga acacagcaac tggcggatga ttaacttgcc ggttggcccg
     4561 ctggtggacg gcaaaccgat ttatacgctc tacatcgcgc tgtctatcga ttttcatctt
     4621 cattacatta atgatttgat gaataaactt attatgaccg catcggtaat cagcattctg
     4681 atcgtcttta tcgtgctgtt ggcggtacat aaaggtcacg cgccgatccg cagcgtcagc
     4741 cgtcaaatcc agaacattac ctcgaaagat ctcgacgttc gcctcgatcc acagaccgtg
     4801 cccattgaac tcgaacaact ggtgctgtcg ttcaaccata tgatcgagcg tattgaggat
     4861 gtctttaccc gccagtccaa tttctcagcg gatatcgccc acgaaattcg tacaccaatt
     4921 acgaatctca taacgcaaac ggaaatcgcc ctcagccagt cgcgcagcca gaaggagctg
     4981 gaagatgtgc tctactctaa tctcgaagag ctgacgcgaa tggcgaaaat ggtcagcgat
     5041 atgttgtttc tcgcccaggc cgataacaac cagttaatcc ccgaaaagaa aatgctcaac
     5101 ctggcggatg aagtcggcaa agtgttcgat tttttcgagg cgttagcgga agatcgcggc
     5161 gtggagttgc ggtttgttgg tgacgagtgt caggttgcgg gcgatccgct gatgttgcgt
     5221 cgggcgttaa gcaacctact ttctaatgcc ctgcgttata cgccaaccgg agagacaatt
     5281 gtcgtgcgct gccagacggt cgatcacctg gtgcaagtta ccgtcgaaaa ccctggtacg
     5341 cccattgcgc ctgagcactt accgcgattg tttgaccgtt tctaccgcat tgatccttcc
     5401 cgccagcgaa aaggcgaagg tagcggtatt ggcctggcga tagtgaaatc gattgttgtc
     5461 gcgcataaag gcacggttgc ggtaacgtca gatgcgcggg gaacaaggtt tgttatcata
     5521 ttacccgctt aatccttcag caaacggcaa cttttataac cagtgtaaaa ataacgtgcc
     5581 gcaataatcc tacctattaa tgataaaaag ctgtcacaat tcataaaaaa ctttaatata
     5641 cgccacccta aacataacca gcgttaatgt aaggtttttg tgtggactgg cttcttgatg
     5701 tttttgctac ctggctctac ggcttaaaag taatcgcgat aacgttagcg gtcatcatgt
     5761 tcatcagcgg gctggacgat ttttttattg atgtcgtcta ctgggtacgc cgcattaaac
     5821 gcaagttgag tgtttatcgc cgctacccgc gaatgagtta ccgcgaactg tataaaccag
     5881 atgaaaaacc gttagcgatt atggttccgg cgtggaatga aacgggcgtc atcggcaata
     5941 tggccgagct ggcggcgacc acgctcgact acgaaaacta tcatatcttt gttggcacct
     6001 accccaacga ccccgatact cagcgtgatg ttgacgaagt gtgcgctcgc ttcccgaacg
     6061 tgcataaggt agtctgcgcg cgtcctggcc ccaccagcaa agccgactgt ctgaacaacg
     6121 tgctggacgc catcacccaa tttgaacgta gcgccaattt cgcttttgct ggttttattc
     6181 tgcatgacgc cgaagatgtg atttcaccga tggaattgcg tctgttcaac tatctggtcg
     6241 agcgtaaaga tctgattcag atcccagtgt atccgttcga acgcgaatgg acgcacttca
     6301 ccagcatgac ttacattgat gagttttcag aactgcatgg caaagatgtt ccggtgcgtg
     6361 aagccctcgc cggacaggtg cccagcgcag gcgtcggcac ctgtttcagc cgccgcgccg
     6421 tgaccgctct gttagctgac ggtgacggta ttgctttcga cgtgcagagt ctgactgaag
     6481 attacgatat tggcttccgc ctgaaagaaa aaggtatgac ggaaattttt gtccgttttc
     6541 cggtggtgga cgaagccaaa gaacgcgagc agcgtaaatt tttacagcac gcacggacgt
     6601 caaacatgat ctgcgtgcgc gaatatttcc ccgatacctt ttcgactgcg gttcgacaaa
     6661 aatctcgctg gatcatcggc attgttttcc agggctttaa aacccacaaa tggacctcca
     6721 gcctgacgct gaactacttt ctctggcgcg accgcaaagg ggcaatcagt aactttgtca
     6781 gcttcctcgc gatgctggtg atgatccagc ttttgctgtt actggcgtat gaaagtttgt
     6841 ggcccgatgc ctggcatttc ctttctattt tcagcggcag cgcatggtta atgaccctgc
     6901 tgtggctaaa ctttggtttg atggttaacc gcatcgtgca gcgggtgatt tttgtcactg
     6961 gctactacgg tctgacgcag gggctgctat ctgtcctgcg tcttttctgg ggtaacctga
     7021 ttaacttcat ggccaactgg cgcgcgttaa aacaggtact tcaacacggc gatccgcgtc
     7081 gagtggcgtg ggataaaaca acgcatgact tccccagcgt gactggcgat acccgctcgt
     7141 tgcgcccgtt aggtcaaatc ctgctggaaa atcaggtcat cactgaagaa caactcgata
     7201 cagcactgcg taatcgcgtc gaaggtctac gcctgggcgg ttcaatgctg atgcaggggc
     7261 tgattagcgc cgagcaactg gcacaggcgc tggcagagca aaacggcgtg gcgtgggaat
     7321 ccatcgatgc ctggcagatc ccttcctcgc tgattgccga aatgccggcc tccgtggcgc
     7381 tgcattatgc ggtactgccg ctgcgtctgg acaatgatga gttaattgtc ggcagtgaag
     7441 atggtattga cccggtttcg ctggcggccc tgacgcgtaa agtcggacgc aaagtgcgtt
     7501 acgtcattgt tctgcgggga caaattgtca ccggattacg ccactggtat gcacgccgac
     7561 gcggtcacga tccgcgggca atgttgtaca atgcagttca gcatcagtgg ctcacggaac
     7621 agcaggccgg tgaaatctgg cggcaatatg tgccgcatca gttcctgttc gccgaaatac
     7681 tgaccacgct cggtcatatt aatcgttcag caattaacgt gttgttattg cgccatgaac
     7741 gcagttctct gccgctcggc aagtttttgg tcaccgaagg cgttatcagc caggaaacgt
     7801 tggatcgcgt cctgacaatt caacgcgaat tacaagtttc gatgcaatca ctattactca
     7861 aagcaggttt aaacacagaa aaggttgcgc aactggagtc cgaaaatgaa ggagaataac
     7921 cttaatcgcg tcatcggatg gtctggttta ctgctgacgt ctttattgag taccagcgca
     7981 ctcgcagaca atatcggcac cagcgcagaa gagctggggc tgagcgatta tcgccatttt
     8041 gttatttatc cccgtctcga caaggcgctg aaggcacaga aaaataacga cgaagcaacc
     8101 gccatccgcg aatttgaata tatacaccaa caggtgccgg ataatattcc actaacttta
     8161 taccttgcgg aagcctatcg ccattttggt catgatgacc gggcgcggct gttgcttgag
     8221 gatcaactga aacgtcaccc aggagatgcc cgacttgagc gcagtctggc ggctattcca
     8281 gttgaagtga aaagcgttac gactgttgaa gaactgcttg cccagcaaaa agcgtgcgac
     8341 gctgcgccga ccctgcgttg tcgcagtgaa gttgggcaga atgccctgcg gctggcacaa
     8401 ttacctgtcg ccagagcgca actgaacgat gcgacgtttg ctgcatcgcc ggaaggaaaa
     8461 acgctgcgaa ccgatctgct gcaacgcgca atctacctga aacaatggtc ccaggcagat
     8521 acgctataca atgaagcacg ccagcagaac acattaagcg cggcagaacg ccgccagtgg
     8581 tttgatgtgc ttcttgccgg gcagctggac gatcggatcc tggcgctgca atcacagggg
     8641 atcttcaccg atccgcagtc atatattact tacgcgaccg cgctggctta tcgtgacgaa
     8701 aaagcacgcc tccagcgtta tctcattgaa aataagccgc tgtttaccac ggacgcacaa
     8761 gagaaaagtt ggctctatct gttatctaaa tacagcgcca accccgttcc ggcgttggcg
     8821 aattatacgg tacagtttgc cgataaccgc cagtatgttg ttggcgcgac gctaccggtg
     8881 ctgttaaaag aaggtcagta cgacgcagcg caaaaactgc tcgccaccct ccccgccaat
     8941 gaaatgcttg aggagcgtta taccgttagc gtagcgaccc ataacaaggc tgaagctctg
     9001 cgtctggcac gattgctgta tcagcaagaa ccggcaaatc ttacccgcct ggatcaactg
     9061 acctggcaac tgatgcagaa cgagcagtca cgcgaagctg ccgatttatt gctgcaacgc
     9121 tatcctttcc agggcgatgc gcgtgtcagc cagacgttaa tggcgcgact ggcgtctctg
     9181 ctggaaagtc atccttacct ggcaacgccg gcgaaggtgg cgattttatc gaaaccctta
     9241 ccgctggcgg agcaacgtca gtggcaaagt cagttgccgg gtattgcaga taattgcccg
     9301 gctatagttc gcttgctggg cgatatgtcg ccttcctacg atgccgccgc ctggaaccgt
     9361 ctggcaaagt gttatcggga cacgctaccc ggtgtggcgt tgtatgcatg gcttcaggcc
     9421 gaacaacgac aaccgaacgc ctggcaacat cgtgcggtag cctatcaggc gtatcaggtt
     9481 gaggactacg ccaccgcact ggcggcctgg cagaaaatca gtcttcacga catgagcaat
     9541 gaggatctgc ttgctgctgc caataccgcc caggcggcag gaaatggcgc aactcgcgat
     9601 cgctggcttc agcaggcaga acaacgtggg ctgggaaaca atgccctcta ctggtggctg
     9661 catgcgcaac gttacattcc tggtcagccg gaactcgcac tgaacgatct cacgcgctca
     9721 atcaatattg cgccttctgc caacgcttac gttgcgcggg cgacaattta tcgccaacgt
     9781 cataatgtcc cggcggcggt gagtgatttg cgcgccgcgc tggaactgga accgaataat
     9841 agcaacaccc aggcagcgct cggttacgcc ttgtgggata gcggtgatat cgcacagtcg
     9901 cgggaaatgc tcgaacaggc gcataaaggg ctaccggacg atccggcact tatccgacaa
     9961 ctggcctacg tgaaccagcg tctggatgac atgcctgcga cgcagcacta cgcccggctg
    10021 gtgattgatg acattgataa tcaggcgctg ataaccccac tgaccccaga gcaaaatcag
    10081 caacgcttca atttccgccg tctgcatgag gaggtcggtc gccgctggac gttcagtttc
    10141 gattcttcta tcggcttgcg ttccggcgca atgagtaccg ctaacaataa tgtcggcggc
    10201 gcagcgccag ggaaaagcta tcgtagctac gggcaactgg aagccgagta tcgcatcgga
    10261 cgcaatatgc tgctggaagg cgacctgctc tcggtttaca gccgtgtatt tgccgatacc
    10321 ggagaaaacg gggtgatgat gccggtgaaa aatccgatgt ccggcaccgg tctgcgctgg
    10381 aagccgctgc gcgatcagat ctttttcctc gccgtcgaac agcagttgcc gctgaacggc
    10441 caaaatggtg catccgatac catgctgcgc gccagcgcct cattctttaa tggcggcaaa
    10501 tacagcgacg aatggcaccc gaacggttca ggctggtttg cccaaaacct gtacctcgat
    10561 gcagcgcaat atatccgcca ggatattcag gcgtggacgg cagattatcg cgtcagctgg
    10621 catcagaagg tagctaacgg acagactatt gagccttacg ctcacgttca ggacaacggc
    10681 tatcgtgata aaggcactca gggcgcgcag cttggcggtg tcggggtccg ctggaatatc
    10741 tggaccggcg agacgcacta cgacgcctgg ccgcacaaag tcagtctcgg cgtcgagtat
    10801 caacatacct ttaaggcgat taatcaacgt aacggagagc gcaacaatgc gtttctcacc
    10861 attggagtgc actggtaaat gcgtaagttc attttcgtat tgctgacact gcttttggtc
    10921 agcccttttt cctttgcgat gaaaggtatt atctggcaac cacaaaaccg tgatagtcag
    10981 gttaccgata cccagtggca ggggctgatg agtcagttac gtttgcaagg cttcgatacc
    11041 ctggttttgc aatggactcg ttacggcgat gcattcaccc agccagaaca gcgcgcgtta
    11101 ttgtttaagc gtgccgcagc tgcgcaacag gctggcctga agctgattgt cgggctgaat
    11161 gccgatccgg aattttttat gcaccagaaa cagtcgtctg cagcgctgga aagctatctt
    11221 aaccgcctgc tggctgccga tctccagcaa gccagattat ggagcgccgt acctggcgta
    11281 acgccggatg gctggtacat cagcgcggaa attgacgacc tgaactggcg cagcgaagcc
    11341 gcccgtcagc ctttgctaac atggttaaac aacgcgcagc ggttgattag cgatgtttca
    11401 gcaaaaccgg tttatatcag tagttttttc gccggaaaca tgtcgcccga tggctatcgc
    11461 caactgctgg aacacgttaa agcaaccggc gttaatgtct gggtacagga tggcagcggc
    11521 gtggataaac tgaccgctga acagcgtgaa cgctatctac aggccagcgc cgattgccaa
    11581 agctccgccc cagcaagcgg cattgtttat gaactgtttg tcgccggcaa aggcaaaacc
    11641 tttacagcga aaccgaaacc ggacgcagaa attgcctcgc tgttagcgaa acgttcctct
    11701 tgcggtaaag acacgctcta tttctctctg cgctatttgc ccgtcgcgca cggcattctc
    11761 gagtattaaa tctcctccag gtaagtcggg tacgacctgg cttacctctt tcgctctcgt
    11821 cagaattcca tcaagattaa ttgctaaaaa gcgtgctaaa gaaagaagat cgttatcggc
    11881 gaacgggccg atcactcatg caggaagcat agatcctttg agcggagttt ctgatatctg
    11941 aggagtgcga aatgcaattg agcagcagtg aaccttgcgt ggtgatcctg accgaaaaag
    12001 aggtagaggt aagcgtcaat aaccatgcta cgtttaccct tccgaaaaac tacctggccg
    12061 ccttcgcgtg caacaataac gtcattgaac tctcaacgtt aaatcacgta ttaatcaccc
    12121 acatcaaccg taacatcatc aacgattatc tgttgttttt aaataagaac ttaacctgta
    12181 taaagccctg gtcgcggctg gcaactccgg ttatcgcttg tcatagccgt acaccggaag
    12241 tgttccggct agccgccaac cacagcaagc agcaacccag caaaccctgc gaagcggagt
    12301 tgacgcgcgc attgcttttt accgtattgt ctaactttct tgagcaatcg cggtttattg
    12361 ccctactgat gtatatctta cgcagcagcg tccgcgacag cgtctgccgc attattcaaa
    12421 gcgatattca gcattactgg aatctgcgaa ttgtcgccag ttcgctatgt ttaagcccca
    12481 gcctgctcaa aaagaaatta aaaaacgaaa ataccagcta tagccagatt gtcacagagt
    12541 gtcgtatgcg ttacgccgta cagatgttat tgatggataa caaaaatatc actcaggtag
    12601 cgcaattatg tggctatagc agcacgtcgt actttatctc tgtttttaag gcgttttacg
    12661 gcctgacacc gttgaattat ctcgccaaac agcgacaaaa agtgatgtgg tgaagggcaa
    12721 agcggaaacg gataagacgg ggataaatga agaagaaatg gcgcgccctg caggattcga
    12781 acctgcggcc cacgacttag aaggtcgttg ctctatccaa ctgagctaag ggcgcgttga
    12841 taccgcaatg cggtgtaatc gcgtgaatta tacggtcaac ccttgctgag tcaatggctt
    12901 ttgatctggt tgctgaacaa gtgaacgacc gcgtctgatt ttctgattta tttcgttata
    12961 gcggcaaaca aacgcacacc gctgcgcgtc tgaatcaaga aaacccgtat tttcatgtat
    13021 caagtacaat ttcccgccct aacggaaaat tgtccgatcc aatgagactg gtaactatga
    13081 aaccaacgtc ggtgatcatt atggatactc atcctatcat cagaatgtct attgaagttc
    13141 tgttgcaaaa aaacaatgaa ttgcaaattg tcctgaaaac ggatgattat cgcataacca
    13201 tcgattatct ccgaacccgt cctgttgatt taatcattat ggatatagac ttgcccggaa
    13261 cagacggttt taccttcctg aaaaggatca aacaaatcca gagtacagtg aaagtattat
    13321 ttttatcatc gaaatcagaa tgcttttatg ctggcagagc gatacaagct ggtgctaacg
    13381 gttttgtcag taaatgcaat gatcagaatg atatttttca tgccgttcag atgatcctct
    13441 ccggatacac tttttttccc agcgaaacgc tcaactatat aaaaagcaat aaatgtagta
    13501 cgaatagttc aacggtcact gtgctatcta atcgtgaagt aaccatatta cgttatctgg
    13561 ttagcggatt atctaataaa gaaattgccg ataagttatt acttagcaat aaaacagtta
    13621 gtgcacataa atctaatatt tatggcaagc taggtttgca ttcaattgta gagcttatcg
    13681 actacgccaa attatatgaa ttaatataat attaattata attgatcata aatatcgcat
    13741 ccgctttcgc cacaccaggc tgaacaccgc tggctaacgc tcgataattg gcaaaaaagt
    13801 ttagtgtgac attgccattt gcatcaactt cctcagtcga gctcgcctcc cccagtgcga
    13861 gccgggagcg atcgctatta cgtaattcga tggcgacggt ttgtgccatt gcgggatcat
    13921 ccagagccag caggttggca tcagatgccg gcgttcccgt aaataaaatc gcaactgaac
    13981 ccggaggaca tccctccagc cgcaggctaa aagggacgag tgccgtggta tcgccagcgc
    14041 tcagtaattg tgtcgtaggc catctgccta aatctaccgt cttatcctta tccgctgtgt
    14101 ttacggtaca ggagaaatca acaacgttac cgtgcaaatt gatattaatc gttcctaaag
    14161 ggtcaactgc ccatccactg gaactccaca gtagcccgca gaaacagcta aagagtactc
    14221 ttctcattct ccgtacctca ttgataatcg acgcgtaaat accccagcgc gctaaacggc
    14281 ccttcggtcg gtttttgacc ggtaatactg ataggccagg cgcgaagtgt gacattggct
    14341 gccgcagctg catccagacg gaaaggaata acgctattga gatcgttagg cgtgatcggc
    14401 gtatcgttct gatcggccac aataaaacct aaatcctgat tgtccgacac catcgcctga
    14461 ccagaaacgg cactggcttc cagacgcatt gttaaataag cctgcgcagc aacattcgta
    14521 catttgatcg caatgctctt ggtttgcggc atgacaccag ctggtcgatt ccccggccct
    14581 gccgcactaa ataacgatgc gccgatatca ccaaaatcaa attcaacaat ttgcccggca
    14641 tttaattcgc agttttgcgg tacttcaacc cggccaccaa aactaatggt ataaacaggt
    14701 gtaaccaacg gatcgttagg cgttgatgtc acatacacgg taaacatcac ctgtctgggg
    14761 atctccacca tgttaataaa gggacgaata acctttaaac gaaatatcaa acgagaatcc
    14821 atcaccccga acggaaggtt ttgcgaaacg ttaggatctg ttcccatacg aatataattt
    14881 tgcggaggga attgaatatc catcacgcta tcaaccagac tcatcgcacc taatagataa
    14941 tcatttaatt gcatatattt ataattatcg atagtttcct gaacaataaa gttggtgaca
    15001 taactacggt atgtataatt caccagcgtg ccgggtggac aaattgctga tacacctacc
    15061 cagccggatt tttccggtaa aacaacaatc tgccctggct ggttattggt gctattaaag
    15121 acatttgaga gatcatagtg ttcatcttct gctacgccag tactatttgc acaaacagta
    15181 gcccaggata tgtttgccag acacaaacat gccattgcca ataataatct gcagattatc
    15241 ttcattgata atttccttta tgagccagga tgtgtgcaaa ctgccgaaat aacagtgacg
    15301 gcttgctgta agctttgctt cggcaataca tagtgcgcta tgcagcgtga gttcgcgccg
    15361 tctccccact gaaccagtaa ttcaccagac aatggcgcac cgcttaaata aacttgcccg
    15421 tcatcaccca ccatactggt tattccggta ctgttttccc gtaccggtga tccaaacggc
    15481 accggttttc cgccctgggt aacggtaatg agcgcccgca cgcctatgcg ggtatcaaaa
    15541 ttggcacgaa ccaacgcgcc ttgcgtcggc acaacgctgc taatattttt ttcaacatcg
    15601 atggagttcc ccatcgtatt ggtatcaagc gcgatacggt tataccgata aaccgtggca
    15661 tacggcatca ccgcatagcc gcgccagtcg gttaaaattc cagtttgatt ttcaatgcgt
    15721 acacctcctg cgcctggcgc tttaatcaaa acattggtat cccctaaagg ctggctcagc
    15781 gttataccat tttcatgccc gaccacaccg ccagacagct gccagttaac gtcatgttga
    15841 tcgcggtcgt agttataccc gacccccagc gtaccgtaag cggcctgcca gtttgccgtg
    15901 gcgctgcccg tatacccatt attcgaggta tcgccctggc ttacgtgata actcaggttg
    15961 tggccttcca gtaaggtccc accaacgcct gccagccagt tattttgccc gttgctgtta
    16021 cggttggcgt taaaggaggc ataagcgcgg tcgagcgcat tttcccgggt gtagcgacgt
    16081 ttggtcagaa cattgaaagg cactgaaaca ttaagtccga caatacgttc gttatcgggg
    16141 atccctacag attcattcca cgaaaatgag agtgaataac tgatgccaac ccagctgctg
    16201 gtatacccca cctgatacca ggtatctgaa tccgaagtat tccagtattt ttgatgggta
    16261 ccagaaatat aaagcgagcc aaagtcatta agtgattgtg aaatatttaa ctgcaaacgg
    16321 tctttacgac taaagcgtaa attgtggtaa ttcacgatta tcggttcatc gcgatgctca
    16381 ccgtcataat cgtaatcata ttcgtacccc tccattcgac gatacgcaac atcatcaagg
    16441 gtataaaaac cttgtgtcga atagcggtaa cccattaact gaaaattggt gccgaaagtg
    16501 ttcattgatt tcgcatagag gaagcgaatg gaatccccct catgacgact gtcgtcggct
    16561 aactgactgc gcgcatgcgt tacatccagc gacactgcgc cccagttccc gaggttgcgc
    16621 cccagcccta acaaaaaggc ggtgtaattt gcagataatt gcgtcccgcc gtaggcagta
    16681 aattcctgtg gtaaaccgcc gagtgccgtg ccctgaaaaa agaaaggcga cgattgctga
    16741 ctattaccgc tacgaaaatc gcccgccgtc aggtcaaatt tgaaacgccc ttcgcgttgt
    16801 aaaattggca ctgttgaata cggaatagtg taattctgct gattaccatc gcgctcgtcg
    16861 atggtgacgt ccagatcgcc atttgaagat gtcgggtgca aatctgtaat ttcaaaagca
    16921 ccgggggaaa catagctttg atagataata aaaccatttt gccgaatcgt tagctgggcc
    16981 gccgtacggg caatcccacg taccgttgga gcaaacccct gctggctatc aggatacata
    17041 ttatcggaag aataaagccg tacacctcga aatccaacgc catcgaaaat atcactccct
    17101 gtattaccgt ctcccattac cagttcactt ttcagcggaa taatggcgcg ctgtacccag
    17161 gtgccaatat tattccactg ttctgaatga tatccatttc cgcgaaaata gttccaggaa
    17221 ccattgttgc gtaaacgcca cgggccaata ttaatcccgc tgagttcact aaaaaaatag
    17281 ctatcgttac cattacctct gttaccggtg aaattataat tcaggagtaa agcaggaata
    17341 ccttcatccc actcttcagg cggaatgtaa ccgtgagcgc tactcaacaa cgctatctga
    17401 ggaatagtga tgttcaggcg catcgctgca aaatcaaagt taattgtggc atcaggtaca
    17461 gctttcagta aattgatgca tttattattt tgctgctggg ctaactcggg gaatgcactg
    17521 ctattaaggc caattcgttc aaggagtgcc tggttaaaac aaggcattaa cccacctgat
    17581 ttatcacctg tatttactgt cgtcgattca aatacgatat cctgtgaacc aatgaactca
    17641 tcattacgcc agagatccac ccgatacacg cccgcaggtt gatgattacc tctctcaaag
    17701 cgcgataaat cagcaacgga tgtgccattc tctaataaaa atgccgggtt gaaataactt
    17761 tccgcatagc ttaaaaagga gggtaaaatg gcaagagatg aataacaaat tccggccaga
    17821 caccaggtat acctctgcgg aatatccgta gtagtgggta ttttcataat tagctctgat
    17881 attgtcagat gattcggtta gtttaagttc acttctctga ccggggttac tgaaccataa
    17941 tcattaacgg tctgaaattt cagctttccc tgcactcctg agggtaaggg aatttgtgaa
    18001 ttaactctgg gtgcagccat agcattcccc agtttttggc tgcccacttg caagttaacc
    18061 agggtgacat aaaaaggtga tggattatga acattgatat agttaccgga tcgcgaaaac
    18121 ttgagtgtgt ccggcgcttc tgcgggtaat tcttgtaatt gaattggacg gagaaataat
    18181 ttcatgcgcg ataaaatcgc cagttgcaaa acattcctgc cgttcaatgc atttttatct
    18241 accgaaggga tcgtcttaac attcatccag aacagagact cacgatctgc cgccagcggt
    18301 ggaccggtgt aaataat
//
LOCUS       DAMZFT010000096        18060 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.096, whole genome shotgun sequence.
ACCESSION   DAMZFT010000096 DAMZFT010000000
VERSION     DAMZFT010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18060)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18060)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18060
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.096"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(237..1259)
                     /locus_tag="PN977_003453"
     CDS             complement(237..1259)
                     /locus_tag="PN977_003453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrD"
                     /protein_id="HDI6043455.1"
                     /translation="MDKRVIFAVAGSGKTTLLIRRLREDRRTLILTFTVNNEAHLRAQ
                     IIRRFGYIPYGIRVMTWFEFLHGFCFRPFLQEQLSSRGLSFNQPPSRIPRTNIRHYQD
                     PAGRLYHRRLAHLLTARGLLPDIRTRLARYYDELFVDEVQDFAGHDFNFLLELCRAEI
                     SVLCCGDFYQHTFDTSRDGNVNATLHEDITRYEARFRAAGIMVDCETLSRTWRCSATV
                     CEFITGQLNIRISAHGTHTTQIEIVTDEARSAALHADNTMIKLFYREHHRYGCHSMNW
                     GGSKGLDHFQDVCIVMGANHWMRLIQQKLAALPPSSRNRLYVACSRARGNIYFIPESH
                     LRRFRN"
     gene            complement(1244..2809)
                     /locus_tag="PN977_003454"
     CDS             complement(1244..2809)
                     /locus_tag="PN977_003454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDI6043456.1"
                     /translation="MPLITRLMLQNFKKFPELDLRFSNDRNILVGDNESGKSTILLAL
                     DLVLSDSRHRVEALGVESLLSQSAVRHFQEGERRADQLPVLTADVFLSNGGEPDLNGR
                     QNLAGIDADGLRMRIAPMMEEYGQDIHHVLQQDPDNFPYEYYSVQFSTFSGGHFAGFR
                     RYLRHLFLDSARIDNEHAAQEYTRTVYSVNVPVADRYRLENSYRQQKMHFCARHLSAI
                     NDTLKTYQFGVRSGAKSGLEANLDITEDGISIRHRGKGRQCFIKTEFALQRHQQQGGE
                     IHALLLEEPENHLSHVSMKRLVNQLATERQTQVFIATHSSHISSRLDLRKAILLGATR
                     PVLMNELSAETAAFFMKAPDNNVLEFALARRVLLVEGDAEFILIEAFYHRLYGRAPED
                     DGVHIIAIGGTSFRRYLELARLLENRVAALRDNDGNYQQNCDERYADVLCSRSRVFAD
                     HDNSRSTFEICLYQDNADLCDALFRGTRRTLTVQDYMLANKAEAAFQLLQLHAEKLTV
                     PDYIQEALAWIRE"
     gene            complement(2884..3300)
                     /locus_tag="PN977_003455"
     CDS             complement(2884..3300)
                     /locus_tag="PN977_003455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HDI6043457.1"
                     /translation="MNKIYMLDTNICSFIMREQPEALLKHLEQSVLRGHRIVVSAITY
                     SEMRFGATGPKASPRHVQLVDAFCERLDAVLPWDRAAVDATTEIKVALRLAGTPIGPN
                     DTAIAGHAIAACAILVTNNVREFERVPGLVLEDWVR"
     gene            complement(3297..3527)
                     /locus_tag="PN977_003456"
     CDS             complement(3297..3527)
                     /locus_tag="PN977_003456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HDI6043458.1"
                     /translation="MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVR
                     PTWGSFLELEKADPDFMTEREDVVSDEGRVNL"
     gene            3908..7702
                     /locus_tag="PN977_003457"
     CDS             3908..7702
                     /locus_tag="PN977_003457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001505097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pcar"
                     /protein_id="HDI6043459.1"
                     /translation="MPVLSANKAQEEQINTSYEPLTPSVITDEKAQSYIEALNFACSR
                     PDIRNIAVTGPYGAGKSSVLLTWERAEDNDFRVMTVSLADFEMQRAYPGDSLSVEGKP
                     DYDDNEKKAGKAEEKTIEYSILQQLLYKEKKSVLPYSRLERISDVSACQIAMMTASLL
                     FILASTATGLLFLFPDYIREKLSLSPELSQFLLELPVLARFGSAGIFLFTALFFALKK
                     LHRTGVFDRRVSIDKVDILKGAITTRPAAPSLLNVYIDEIVYFFEQTQYNVVIFEDLD
                     RHNDGAIFIKLREINQLINNCLPTDNPVRFIYAVRDNLFITPESRTKFFDFVIPVIPV
                     MDSENASEHFLSKFTLDELRQEGFKDCLARLALFIPDMRVMHNIANEFRLYRNIVNNG
                     GDLKRLISLITYKNLYAEDYHRIDQKKGMLYSIVSEYTSGKLREDYCNNLKNKIETCL
                     TELSKLHNEEVVTEHELRSEILRPYISEKTAPRLHIMTGTGGQYDFDDVIQNENTFLS
                     LLSNQIISIKPSGYNITITTIDKKTAQVMKEEYQERIAIIQKKLNNDVSRLEENIKKN
                     RCEIQNASSYDLAFFINKMGRSGFERYIACCSTPEQHDGESITDNAANIDFIYFLLSH
                     GYLSTDYMAYRSVFMPGSLSTEDNNFIRAVTSGRLPDETAKMPLSNIANTVAKLHGLG
                     ILMHDNAWHPQILWYLMRNDTNSLKTIMRMQAEVGAERRMVRLANEIFPLWEPAAQRE
                     YIRLMVDGDGHLSTMIHQIGRLNDTVAEQNLLPVLLSLPILSWEAVSQITREELQRLI
                     DLQFNLVTSLPENCAQFFCENLRNSGCRLTNIPLARSDSGQETLHLVVQKKLWTYSTL
                     NLQNICFSLSHESENNSDTFRKKPVALIKSLRIPNLEKYVYENISSFIRDVFIHSEEN
                     DLIPDFLNSTFVDWDDAKYMTESMSFVLEDVSVILNKENTETTEISYDQNLYSLLAHH
                     NHITPCWNNVISLLSEDASIAGDTFCEWLNINYSLLPNDSLPLTDVQFSQLLIKAVTS
                     PHISKEALIAITMAFRITLINVPENLPLNNAAVLIKQKWLAPTSTVFEQLYQALYEEG
                     DKLTSLLYALICARPVLLSDNYELVLFSDDQFDLGITRLILNGDKIADEVCISILNWL
                     WEKDEALLSEAPLLSQQALIRFSTKITDDRQKQALLMQCLKNDGGSHKFIRQVLMTFG
                     HQDYAAFLTERNYRSIPRSDAMWQLAVQLGNSGFIRPPKLTHADTRIRIEPFFNAENE
                     YD"
     gene            complement(7747..8163)
                     /locus_tag="PN977_003458"
     CDS             complement(7747..8163)
                     /locus_tag="PN977_003458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001505096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HDI6043460.1"
                     /translation="MNKIYMLDTNICSFIMREQPEAVLKNLEQAVLRGHRIVVSAITY
                     SEMRFGATGPKASPRHVQLVDAFCARLDAILPWDRAAVDATTEVKVALRLAGTPIGPN
                     DTAIAGHAIATGAILVTNNVREFERVPGLVLEDWAG"
     gene            complement(8160..8390)
                     /locus_tag="PN977_003459"
     CDS             complement(8160..8390)
                     /locus_tag="PN977_003459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HDI6043461.1"
                     /translation="MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDRIILRPLR
                     PSWSSFLELEKADPGFMTEREDVVCDEGRFNL"
     gene            8655..9155
                     /locus_tag="PN977_003460"
     CDS             8655..9155
                     /locus_tag="PN977_003460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000528931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6043462.1"
                     /translation="MGNYSDFETDFVQRTLALIDQYNEMIEVLGKPFREQYNYTLTLN
                     CLLGLIVLPKERALSFLLADRLTRQLKAEMGLHESQLPGPEMNLRELIHKMRNSVAHF
                     CVQVESASDAHLVDWIVFRESQKDGEVYASFSAPELLPFLKYYATLLLDNMARRRAPD
                     VNILDL"
     gene            9168..9941
                     /locus_tag="PN977_003461"
     CDS             9168..9941
                     /locus_tag="PN977_003461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000905949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6043463.1"
                     /translation="MLILDCSSRTQALHTLSAGFGCSPEKLKKVLLSLDLESIYELNP
                     RQLVDAPQYLREYVCAELGEPGPFTRALWFHGTRTFAGNTFPAGLLALNQSESLAMKM
                     LLDLAPNEMVRTHLKEWDVPGGVPDEMFQLRTGDKIHWGPFGHLVRELHFNASENGLH
                     DYLWLPELVEDVCKAYQKKYGHDLKPHYLSVLHPCIVWFEADIVYEKGVLETALSYAY
                     TSVRDLPPDGNATFGIDCDGKSVSRSAIARIEFLQPGQM"
     gene            10108..11241
                     /locus_tag="PN977_003462"
     CDS             10108..11241
                     /locus_tag="PN977_003462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000545985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3800 domain-containing protein"
                     /protein_id="HDI6043464.1"
                     /translation="MHFYVDETGQTGRNLFDKTQPVLSYGVLSSDANLDKVAEADLAV
                     IRKTLGVQRLHAAELGLHRLSDLVDTLLVLQKKHRIRFDIWQVVKRDHAIISFFDQVF
                     DQGMNPAVPWSAYWTPLRYPLLLNLASLFDDELASNAWTARLEAHDERASELFCTVSD
                     ELISRTAASALDHRSKQLITDALNWASANFEQLGYNCKTNKERLRIMPNMIGFQSVLH
                     GICSRLGAPERKASIIVDQQSQFNTTQRELNEFYYQIRDMPWELGPGLPVMNMKNMPA
                     EPLVFQSGTKSAGLELVDIYLWTFKRFMEDKALTKPLSRLVYTNLKTARTNSVSIQSV
                     ASRFKELLGKLPVPSAEIMRLAQELRDFDEARRMPYVVSGSPD"
     gene            complement(11275..11787)
                     /locus_tag="PN977_003463"
     CDS             complement(11275..11787)
                     /locus_tag="PN977_003463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6043465.1"
                     /translation="MTIFKENIVDTSLIGPILTALLAAAGFYCKFWFTRYQASRDQAQ
                     KVSRAIEDVLSKMAALFGQKKPARILREEFSAVIAPLHQEMRILDDMTSRLPLKWLQR
                     EQRHVLYHARWLQRYLDSRRGGSDGDFFLLLHDVAMGTDYQVADILASQHDCKQRRGS
                     QLPVNLKTCP"
     gene            12354..12572
                     /gene="ccdA"
                     /locus_tag="PN977_003464"
     CDS             12354..12572
                     /gene="ccdA"
                     /locus_tag="PN977_003464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin CcdA"
                     /protein_id="HDI6043466.1"
                     /translation="MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERW
                     KAENQEGMAEVARFIEMNGSFADENRDW"
     gene            12574..12879
                     /gene="ccdB"
                     /locus_tag="PN977_003465"
     CDS             12574..12879
                     /gene="ccdB"
                     /locus_tag="PN977_003465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin CcdB"
                     /protein_id="HDI6043467.1"
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSD
                     KVSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     gene            12880..13686
                     /locus_tag="PN977_003466"
     CDS             12880..13686
                     /locus_tag="PN977_003466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage integrase family protein"
                     /protein_id="HDI6043468.1"
                     /translation="MSGSVIHSQSAVMVPAVYSAGQPASLPVAIDYPAALALRQMSMV
                     HDELPKYLLAPEVSALLHYVPDLHRKMLLATLWNTGARINEALALTRGDFSLAPPYPF
                     VQLATLKQRTEKAARTAGRMPAGQQTHRLVPLSDSWYVSQLQTMVATLKIPMERRNRR
                     TGRTEKARIWEVTDRTVRTWIGEAVAAAAADGVTFSVPVTPHTFRHSYAMHMLYAGIP
                     LKVLQSLMGHKSISSTEVYTKVFALDVAARHRVQFAMPESDAVAMLKQLS"
     gene            14460..15215
                     /gene="repE"
                     /locus_tag="PN977_003467"
     CDS             14460..15215
                     /gene="repE"
                     /locus_tag="PN977_003467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000852146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein RepE"
                     /protein_id="HDI6043469.1"
                     /translation="MAETAVINHKKRKNSPRIVQSNDLTEAAYSLSRDQKRMLYLFVD
                     QIRKSDGTLQEHDGICEIHVAKYAEIFGLTSAEASKDIRQALKSFAGKEVVFYRPEED
                     AGDEKGYESFPWFIKRAHSPSRGLYSVHINPYLIPFFIGLQNRFTQFRLSETKEITNP
                     YAMRLYESLCQYRKPDGSGIVSLKIDWIIERYQLPQSYQRMPDFRRRFLQVCVNEINS
                     RTPMRLSYIEKKKGRQTTHIVFSFRDITSMTTG"
     gene            15803..16969
                     /gene="sopA"
                     /locus_tag="PN977_003468"
     CDS             15803..16969
                     /gene="sopA"
                     /locus_tag="PN977_003468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid-partitioning protein SopA"
                     /protein_id="HDI6043470.1"
                     /translation="MKLMETLNQCINAGHEMTKAIAIAQFNDDSPEARKITRRWRIGE
                     AADLVGVSSQAIRDAEKAGRLPHPDMEIRGRVEQRVGYTIEQINHMRDVFGTRLRRAE
                     DVFPPVIGVAAHKGGVYKTSVSVHLAQDLALKGLRVLLVEGNDPQGTASMYHGWVPDL
                     HIHAEDTLLPFYLGEKDDVTYAIKPTCWPGLDIIPSCLALHRIETELMGKFDEGKLPT
                     DPHLMLRLAIETVAHDYDVIVIDSAPNLGIGTINVVCAADVLIVPTPAELFDYTSALQ
                     FFDMLRDLLKNVDLKGFEPDVRILLTKYSNSNGSQSPWMEEQIRDAWGSMVLKNVVRE
                     TDEVGKGQIRMRTVFEQAIDQRSSTGAWRNALSIWEPVCNEIFDRLIKPRWEIR"
     gene            16969..17940
                     /locus_tag="PN977_003469"
     CDS             16969..17940
                     /locus_tag="PN977_003469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family plasmid partition
                     protein"
                     /protein_id="HDI6043471.1"
                     /translation="MKRAPVIPKHTLNTQPVGDTSLSTPAAPMVDSLIARVGVMARGN
                     AITLPVCGRDVKFTLEVLRGDSVEKTSRVWSGNERDQELLTEDSLDDLIPSFLLTGQQ
                     TPAFGRRVSGVIEIADGSRRRKAAALTESDYRVLVGELDDEQMAALSRLGNDYRPTSA
                     YERGQRYASRLQNEFAGNISALADAENISRKIITRCINTAKLPKSVVALFSHPGELSA
                     RSGDALQKAFTDKEELLKQQASNLHEQKKAGVIFEAEEVITLLTSVLKTSSASRTSLS
                     SRHQFAPGATVLYKGDKMVLNLDRSRVPTECIEKIEAILKELEKPAP"
BASE COUNT         4589 a         4266 c         4441 g         4764 t
ORIGIN      
        1 gcttcctggg gggttaatgg ctgatgacga taaaatcata tatcgaatga tttatttctc
       61 tgcctgtaaa tatcaggcca ggatttatta tgcgattaat gataaatatc atatcatctg
      121 cgggtttccc tccacgcagg tccggtttcc ggcgaaaccg gagcctcatt tttcggcctg
      181 aagttttgtc gttgactact ttttaccggc ttgtgcccag ttgagaagcc gctgacttag
      241 tttctgaatc tccgaagatg gctttccgga ataaaataga tgttgccccg tgcccgtgag
      301 caggccacgt atagcctgtt acgggatgat ggtggaagtg ctgccagctt ctgttggatg
      361 aggcgcatcc agtggttggc ccccatcacg atgcagacgt cctgaaagtg gtcgagtcct
      421 ttactgcccc cccagttcat tgagtggcat ccgtaacggt gatgttcgcg atagaagagc
      481 ttgatcatcg tgttgtccgc gtgcagggcc gctgaacgcg cttcatccgt gacgatttca
      541 atctgggtgg tatgtgttcc atgagccgaa atgcgaatgt tcagttgtcc ggtaatgaac
      601 tcacacactg tagctgaaca gcgccatgtc cggctgagcg tctcacaatc caccatgata
      661 cccgcagccc tgaagcgagc ctcgtatcgg gttatgtctt catgcagcgt agcattgacg
      721 ttaccgtcgc ggcttgtgtc aaaggtatgc tgataaaaat ccccgcagca cagcactgag
      781 atttccgcgc ggcatagctc cagcagaaaa ttaaagtcat ggccggcaaa gtcctggact
      841 tcatcaacga acaactcatc gtagtaacgg gcaagtcggg tacggatatc tggcagcaac
      901 ccccgggctg tcagcaggtg tgcgagccgc cggtgataca gccgtccggc gggatcctga
      961 taatgccgga tattcgtgcg cggtattctg gaagggggct ggttgaagct cagaccacgc
     1021 gatgacagtt gctcctgcag gaaagggcga aaacagaaac cgtgcagaaa ctcaaaccag
     1081 gtcatcacac ggatcccgta cggtatgtag ccaaagcgtc tgataatctg cgcccgcaga
     1141 tgtgcttcat tatttacggt gaaggtcagg atcagcgtcc gccggtcctc tcttaaccga
     1201 cgtatcagca gcgtggtttt acccgagccg gcaaccgcaa atatcactct cttatccatg
     1261 ccagtgcctc ctggatataa tcaggaacgg tcagcttttc ggcgtgcaac tgtaacagct
     1321 ggaatgctgc ttctgcctta ttggccagca tgtaatcctg aaccgtgagt gtccggcggg
     1381 ttccgcggaa aagcgcatca cacaggtcgg cattatcctg atacaggcag atttcaaatg
     1441 tggagcggct gttgtcgtgg tcagcaaata cccgggagcg ggaacaaagc acgtcagcat
     1501 agcgttcatc gcaattttgc tgatagttgc cgtcattgtc gcgaagggcc gcgacacgat
     1561 tttccagaag acgggccagt tcaagataac gacggaaact cgttccgccg atagcgatga
     1621 tgtgaacccc gtcatcctcc ggtgccctgc catacaggcg gtgataaaag gcctcgatca
     1681 gaataaactc agcatccccc tccacgagca gcacgcgtct ggccagcgca aattccagca
     1741 cgttattatc cggtgctttc atgaagaagg cggcagtctc agcggaaagt tcattcatca
     1801 gtaccggccg ggtagctccg aggagaatag ccttacgtag gtcgaggcgg gaggagatat
     1861 ggctactgtg tgtggcaata aacacctgcg tctgccgttc cgtggcaagc tgattaacca
     1921 gcctcttcat actgacatgg ctgagatgat tctccggctc ctcaagcaac agcgcgtgga
     1981 tctctccccc ctgctgctga tgccgctgca gcgcaaactc cgttttgatg aaacactgtc
     2041 ttccttttcc gcggtgacga atggaaatac cgtcctcagt aatgtccagg ttcgcctcaa
     2101 gacctgattt agcgccggaa cgcacgccaa actgatacgt tttcagcgtg tcgtttatgg
     2161 cggacagatg ccgggcgcag aagtgcattt tctgttgccg gtaactgttt tcaagccggt
     2221 accgatcggc caccggcaca ttgacgctgt aaactgtgcg cgtatactcc tgtgcggcat
     2281 gctcgttatc aatgcgtgca ctgtctaaga acagatggcg cagatagcgt ctgaagcctg
     2341 cgaaatgacc accggagaat gtgctgaact gcacggaata gtattcatag ggaaaattat
     2401 caggatcctg ctgcaaaaca tggtgtatat cctgaccgta ttcctccatc atgggagcaa
     2461 tgcgcattct gagaccatcc gcatcgatac ccgccagatt ttgcctgccg ttcaggtcag
     2521 gctctccccc attactcagg aaaacgtcag cggtcagcac gggtaactga tcggcacggc
     2581 gctcaccttc ctgaaaatgc ctgactgctg actgtgacag gagcgattcc acccccagcg
     2641 cctcaacccg atgcctgctg tcactcagca caaggtcaag agccagcaga atagtgcttt
     2701 tcccggactc attgtcccca accagaatgt tacgatcgtt gctgaagcgc aggtccagtt
     2761 ccggaaactt tttgaagttt tgcagcataa gccgcgtgat cagtggcatt tttttattcc
     2821 tttatatacc gctgcctgaa agagcggccg gccgcgcctg accgcctgtt ttgttttaca
     2881 gcgttatctt acccagtctt ccagcacaag acccggtacc cgctcaaact ctctcacatt
     2941 attcgtcacc agaatggcac aggcagcgat ggcgtgtccg gctatcgccg tgtcgttcgg
     3001 accgatcggc gtgccggcga gacgcagtgc caccttaatc tccgtggtcg catccaccgc
     3061 ggcgcggtcc cacggcagga cggcatcgag cctctcacag aacgcgtcaa ccagttgcac
     3121 gtggcgtggc gaggccttcg gaccggtggc accgaagcgc atctcggagt aggtgatggc
     3181 cgagaccacg atacggtgac cgcgcagcac cgactgctcc aggtgcttaa gcaatgcttc
     3241 aggttgctcg cgcatgatga acgagcagat gttcgtgtcg agcatataga tcttgttcac
     3301 aggttaactc gtccttcgtc gctgacaacg tcctcgcgct ccgtcataaa gtccggatcg
     3361 gccttttcta gctccaggaa tgagccccag gtcggccgga cggggcgcag aatgatgctg
     3421 tccccttccc ggacgatctc cagctcgctc accccctcaa aatccagatc gcgtggaagg
     3481 cggatagcac ggttgttgcc atttttaaaa atggatacgg ttctcatgat tctctcccgg
     3541 gtaagtgtgg atatgtgatg catatccgga atgatgttca ttgcaccggg tgtgtgcata
     3601 tgtttagtat aggcagatgt taggcatatg tatagattca gcatatgcag atggtgagga
     3661 tatgcgtata ccatgaatat gcagatgtca ggcatatgga tatgttcagc ctatgttatt
     3721 ttctctcttt ccccgtgcag atccggtttc tggtgaagtc ggagcccacc atctccgagt
     3781 atttgataag ccaattcttg tatccctttg cctttattat ctttttggtt ttcagaagtt
     3841 ataatgcctg ttaaatcaac aatgttggaa gatcaacatg agaagaccat cccgaactga
     3901 agcactgatg ccagtactga gtgctaacaa agctcaggaa gaacagatta acacctccta
     3961 tgaaccactg actccctcag tcattacaga tgaaaaagca caaagctata tcgaagcttt
     4021 gaattttgcc tgttctcgcc cggatatcag aaatattgca gtaacagggc cgtacggggc
     4081 cggtaaaagt tcggtattac tgacatggga aagggcggag gataatgact ttcgggtgat
     4141 gaccgtatca ctggctgact ttgaaatgca gcgggcatac cccggagatt cgctgtcagt
     4201 tgagggtaag cccgactatg atgataacga aaaaaaagcg ggaaaagccg aagagaaaac
     4261 aatcgagtac agcatcctgc aacaactgct ctataaagag aaaaaaagtg tgttacccta
     4321 ttcccgtctg gagcgcattt ctgacgtaag tgcgtgtcag atagccatga tgacggccag
     4381 tctgttattc atactggctt cgacggcaac tggtctgctt tttcttttcc cggattacat
     4441 ccgggaaaag ctctccctgt cgccagaact gagtcagttt ctgctggagt tgcctgtact
     4501 cgctaggttt ggtagcgccg ggatatttct ttttactgct ttattctttg cactgaagaa
     4561 attgcaccgt acaggggtat ttgatcggcg tgtaagtatt gataaagttg acatactgaa
     4621 aggtgccatt acaacacgcc ctgcggcccc ttctcttctc aatgtgtaca ttgatgaaat
     4681 cgtctacttt ttcgaacaga cacaatacaa tgtagtcata tttgaggatc ttgaccgtca
     4741 taatgacgga gccattttta tcaaactccg ggaaataaat cagcttatca ataactgcct
     4801 ccccacagac aatcctgtcc gatttatcta cgctgtcagg gataatcttt tcataacacc
     4861 agaatcacgg acaaaatttt tcgatttcgt cataccagta atcccagtaa tggacagtga
     4921 gaatgcttct gagcattttc taagtaaatt tacacttgat gaactcaggc aggaaggatt
     4981 caaagactgc cttgccagac tggctctttt tattcctgat atgcgggtta tgcataacat
     5041 tgcgaatgaa tttcgtcttt accggaatat tgtgaataat ggaggagatt taaagcgtct
     5101 tatttcactg atcacctata aaaaccttta tgctgaagac tatcacagga tagatcagaa
     5161 aaaaggaatg ctttatagca ttgtatctga atatacatca ggtaaattaa gagaggatta
     5221 ttgcaataat ctaaaaaaca aaattgaaac gtgtctaact gaactatcta aactacataa
     5281 tgaagaagtg gtcactgaac atgagttaag aagtgaaatt ttgcggccat atatcagtga
     5341 aaaaacagca ccccgattac atattatgac aggtacagga gggcaatatg attttgatga
     5401 tgtaatccaa aatgaaaata catttctttc tttgctgagc aaccaaataa tcagcattaa
     5461 acctagtgga tataacatta ctataacaac aatcgataaa aaaacagctc aggttatgaa
     5521 ggaggaatat caggaaagaa tagcgattat tcaaaagaaa ctaaataatg atgtttctcg
     5581 attggaggaa aatattaaaa aaaacaggtg cgagattcag aatgcatcct cgtatgatct
     5641 tgcttttttc atcaataaaa tggggagatc aggtttcgaa agatatattg catgttgttc
     5701 aacgcctgaa caacatgacg gagaatccat tactgataat gccgcaaata ttgattttat
     5761 ttatttcctt ctatctcatg gttatctttc cactgactat atggcctacc gctcagtctt
     5821 tatgccaggt agtctgagca cggaagacaa taactttatc agggctgtga cgtcaggaag
     5881 attacctgat gaaacggcaa aaatgcccct cagcaatatt gctaacacag tagcaaaatt
     5941 gcatggactg ggcatactga tgcatgacaa tgcatggcat cctcagattc tctggtacct
     6001 catgcgcaat gatacgaact ctctgaagac tatcatgagg atgcaggcag aggtcggagc
     6061 cgaacggcgg atggtaagac tggccaatga gatcttcccc ctatgggaac ctgctgcgca
     6121 gcgggaatat atcagattaa tggtcgacgg ggatggtcat ttatccacta tgatccacca
     6181 gattgggcgc ctgaatgata ccgtagctga acaaaatctt ctcccggtat tactgtcctt
     6241 accgatactt tcttgggaag ccgtctctca gataactcgg gaagaactgc agagactcat
     6301 cgatttgcaa tttaatctcg taacgtcctt accggagaat tgtgcgcagt ttttctgtga
     6361 aaacctgcgg aattcaggtt gtcgcctgac taacattcct ttggcccgga gtgactctgg
     6421 gcaggaaaca ttgcatttgg tggtacagaa aaaattgtgg acctattcca cgttaaacct
     6481 gcaaaacatt tgtttctcac tttctcatga gtcagagaat aacagtgata ccttccgtaa
     6541 gaagcctgtt gctttaataa aatcgcttcg tattccgaac cttgaaaaat atgtttatga
     6601 aaacatatca tcattcattc gtgatgtttt tatacactct gaagaaaatg atctcattcc
     6661 tgattttctt aattcgacat tcgttgactg ggatgatgca aagtacatga cagaaagtat
     6721 gagcttcgta ctggaagacg tatcagttat cctgaacaaa gagaataccg aaacaacaga
     6781 aatttcttat gatcaaaacc tgtattcact tctggctcat cataatcata ttaccccttg
     6841 ctggaataat gttatttctc tactgagtga agatgcgtca attgctggtg atactttttg
     6901 tgagtggctt aatattaatt attctttatt gccaaatgac tcgttgcctt tgactgatgt
     6961 tcagttctcg caattactta ttaaggctgt tacatcacct catatcagta aagaagcgtt
     7021 aatagcgata accatggcat ttaggataac tcttattaat gtgccagaaa acttaccact
     7081 gaataatgct gcggtactaa tcaagcaaaa atggctggca ccaaccagta ctgtgtttga
     7141 acaactttat caggcactgt acgaagaagg tgacaaactg acatcactac tttatgcact
     7201 tatttgtgcc cgtcctgtat tattgagtga taattacgag cttgttttat tttctgatga
     7261 ccagtttgac ctggggatta ctcgtttaat tctgaatggc gataagattg cagatgaagt
     7321 ctgtatcagt atcctgaact ggctctggga aaaagatgaa gccctgctct ctgaggcacc
     7381 acttctcagc cagcaagccc tgataaggtt ctccaccaaa ataaccgatg accggcagaa
     7441 gcaggcactg ctgatgcaat gtcttaaaaa tgacggtggg tcacacaagt tcatcagaca
     7501 ggtgctcatg acgttcgggc atcaggatta tgccgctttc ctcacagaga ggaattatcg
     7561 tagcattccc cgtagtgatg ccatgtggca actggctgtc cagctgggaa atagtggatt
     7621 tatcaggccg ccgaaactta ctcacgctga tacacgtatt cgtatagagc ccttttttaa
     7681 cgctgaaaat gaatacgact gacatcgcaa aatagtggta agtccgtcgg gggcggcctt
     7741 actcctttat cctgcccagt cttccagtac cagtccaggc acccgctcaa actctctcac
     7801 attattcgtc accagtattg ccccggtggc gatggcgtgc ccggcgatcg ccgtgtcgtt
     7861 tggaccgatc ggcgtgccgg cgaggcgcag cgccacctta acctccgtgg ttgcatccac
     7921 cgcagcgcga tcccagggca ggatggcatc gagacgggcg cagaacgcgt caaccagctg
     7981 cacgtggcgt ggcgatgcct ttggaccggt ggcgccgaag cgcatctcgg agtaggtgat
     8041 ggccgagacc acgatacggt gaccgcgcag caccgcctgc tccagattct taagcactgc
     8101 ttcaggctgc tcgcgcatga tgaacgagca gatgttcgtg tcgagcatat agatcttgtt
     8161 cacaggttaa atcgtccttc gtcgcagaca acgtcctcgc gttccgtcat aaagcccgga
     8221 tcggcctttt ctagctccag gaatgagctc cagctcgggc ggaggggacg cagaatgatt
     8281 ctgtcccctt cccgtacaat ctccagctca ctcaccccct caaaatccag atcgcggggc
     8341 agacggatcg cgcggttatt gccatttttg aaaatagata cggttctcat gtcttcctcc
     8401 tggcaaggcg gcttttatat atgtttagta tatgcagatg tgatacatat gtatatgtta
     8461 taaatatgca gatgtgtgag atatgcgtat gcctgtcata tgcagatgcc aaacatatgg
     8521 atatgcatgg tatctgcatg tttctctctt ttttcaccgg gatccgaaaa atatcatagt
     8581 aggaaatgcc gtacctgagc cgtcaggctg gccggatgat atagtggatt ctcgaaccgg
     8641 aacgggaggg ttaaatggga aattattcag attttgagac cgattttgtt cagcgaacac
     8701 tggcgcttat cgatcaatat aacgaaatga ttgaggtgct gggcaaacca ttcagggagc
     8761 agtacaacta cacgttgacc ctgaattgcc tcctgggtct tatcgtgtta ccgaaagaga
     8821 gagcgctctc tttcctgctt gccgaccgtt taacgcggca acttaaagct gaaatgggat
     8881 tacatgagtc acagctgccc ggaccggaaa tgaacctgcg ggagttgatc cataagatgc
     8941 ggaattcagt tgctcatttc tgcgtccagg tagagtcagc cagcgatgca caccttgtag
     9001 actggattgt tttcagggaa tctcagaaag atggagaagt ctacgccagc ttcagcgctc
     9061 cggagctttt accctttctg aaatattatg cgaccctgct gctcgacaat atggcccgcc
     9121 gtcgagcccc cgatgttaac atattggatt tgtaagggat ttacaccatg ttgattctcg
     9181 attgctcctc acgaactcag gcgttgcata cattatctgc cgggtttggc tgttcccctg
     9241 aaaagctgaa aaaagtgctg ctttcgctcg atcttgaaag tatctacgag cttaatcctc
     9301 gccagttggt tgatgcacct cagtatctga gagaatacgt ctgtgcggaa ctgggcgaac
     9361 cgggaccatt cacccgggct ctgtggtttc atggcacccg gactttcgcc ggtaatactt
     9421 ttccggcggg gttactggcg ctaaatcagt cagaatccct cgccatgaaa atgctgcttg
     9481 acctggcccc gaatgagatg gtccgtacac acctgaagga atgggatgtt ccggggggcg
     9541 ttccggatga gatgtttcag ctcagaaccg gcgataaaat ccactggggg ccttttggcc
     9601 atttagtccg ggaattacat tttaacgcca gtgaaaatgg actgcatgat tacctctggc
     9661 tgccggagct ggtagaggat gtctgtaagg cctaccagaa gaaatacggc cacgatctta
     9721 aaccgcatta tctgagcgtg cttcatccct gtattgtctg gttcgaggcg gatattgttt
     9781 atgaaaaagg agttcttgaa acggctctgt catatgccta tacatcagtc cgtgacctcc
     9841 cgccggacgg aaacgcaact ttcggcatag actgtgacgg gaaatctgtg tctaggtcgg
     9901 ccattgccag aatcgagttt ctgcagcccg ggcagatgta atccctaaaa aacgggtctg
     9961 tgatccggcc cgtttgttca ttgccgtgaa cccagtattg aggtcattaa agccttaagt
    10021 ggatcacttg cagctggctg cagaaaggtt ttacattgac ccggattatg ccgtttccct
    10081 tcctcctgcc tggtaaagga tctctgaatg cacttttatg tggatgaaac aggccagact
    10141 ggccgaaacc tttttgacaa aacccagcca gtcctctcct acggagtgct gtcgtctgat
    10201 gctaatctgg ataaggttgc tgaagcggat ctagccgtaa tacgcaaaac gcttggcgtg
    10261 cagcgcctgc acgctgcgga actgggcttg caccgccttt ctgaccttgt agatacgctg
    10321 ctggttctgc agaaaaagca ccggatacgg tttgacatct ggcaggtagt aaagcgcgat
    10381 catgccatta tttcgttctt cgaccaggta tttgatcagg gaatgaatcc agccgtaccc
    10441 tggtctgcat actggacccc tctgcgctac cccctgctgc tgaatctggc cagcctgttt
    10501 gatgatgaac ttgcttcaaa cgcatggacg gcaaggctgg aggcgcatga tgagcgtgcg
    10561 tcggaacttt tttgtaccgt cagtgatgaa ctgatcagca ggacggcagc cagcgcgctg
    10621 gaccaccggt cgaagcagct gatcaccgat gccctgaact gggcgtcagc caattttgaa
    10681 cagttgggct ataactgtaa aaccaacaag gaaaggctgc ggattatgcc caatatgatt
    10741 ggctttcagt ccgtattgca cgggatctgt tcccgactgg gggcaccaga aagaaaggcc
    10801 agtatcattg ttgaccagca gtcacagttc aacaccaccc agcgtgaact taacgagttt
    10861 tactaccaga tacgggatat gccctgggaa ctggggcccg gattacctgt tatgaacatg
    10921 aaaaatatgc cggctgaacc acttgtattc cagtccggta ccaagagtgc aggtctggag
    10981 ttggtagata tttatctctg gacattcaag cgattcatgg aagacaaggc gctgacaaag
    11041 ccactttccc gccttgttta taccaatctg aaaacagcca ggaccaacag cgtgtcaatc
    11101 cagtcggttg ccagtcgatt taaggaactc ctgggtaaac tcccggtacc aagcgcagag
    11161 atcatgaggc tggcgcagga gctgcgggac tttgatgaag ccagacgcat gccatatgtg
    11221 gtgtcgggat cacccgactg atgccggata ttatctgctt tcaactgccc cctcttaggg
    11281 gcaggttttc aggttaaccg gcagctgtga tccacgacgc tgcttacagt catgctgcga
    11341 tgccagaatg tcagcaacct gataatcggt ccccatcgcc acatcgtgca atagcaggaa
    11401 aaagtccccg tcactgccgc ccctgcgcga atccagatac cgctgcagcc atcgggcatg
    11461 ataaagaacg tggcgctgtt cgcgctgcag ccatttcagc ggcagacggg acgtcatatc
    11521 gtcaaggata cgcatttcct gatgaagcgg cgcaataaca gcggaaaatt cctctctcag
    11581 tatccgggca ggtttcttct gtccgaacag cgcggccatt ttgctcagga catcctcgat
    11641 cgcccggctg actttctggg cctgatcacg ggaggcctga taacgcgtaa accaaaactt
    11701 acagtagaac cccgcagcgg caagcagcgc cgtaaggatc ggtccaatca gacttgtgtc
    11761 cacaatattc tccttaaaga tggtcatgag gccacctgaa gcagccgatg acgcaaatat
    11821 actccgtttt agcaattagt gctatgcgag aatgcaaaaa gtgctattcg ttgttttttg
    11881 tgttgacatt ttaatttatg tgtgagtttt tgttttcttt cagttttctc cactgagatc
    11941 cgaaaacccc aagttacgga tcttcctctc cctccgcaca gcgttacatc ccgtcagcac
    12001 agcatgtagt gcctcataca gttgcccatg gcactatatg ttgtgttgta tctctggact
    12061 gtgatgcgcc gcgcaggggc ggaaaacagc gatatgatga ttttctcagc gttgtacact
    12121 tccggaaagt cgtttattca aataaagtcg gatccatacg aaacgggaat gcggtaatta
    12181 cgctttgttt ttataagtca gattttaatt tttattggtt aacataacga aaggtaaaat
    12241 acataaggct tactaaaagc cagatagcag tatgcgtatt tgcgcgctga tttttgcggc
    12301 ataagaatat atactgatat gtatacccga agtatgtcaa aaagaggtgt gctatgaagc
    12361 agcgtattac agtgacagtt gacagcgaca gctatcagtt gctcaaggca tatgatgtca
    12421 atatctccgg tctggtaagc acaaccatgc agaatgaagc ccgtcgtctg cgtgccgaac
    12481 gctggaaagc ggaaaatcag gaagggatgg ctgaggtcgc ccggtttatt gaaatgaacg
    12541 gctcttttgc tgacgagaac agggactggt gaaatgcagt ttaaggttta cacctataaa
    12601 agagagagcc gttatcgtct gtttgtggat gtacagagtg atattattga cacgcccggg
    12661 cgacggatgg tgatccccct ggccagtgca cgtctgctgt cagataaagt ctcccgtgaa
    12721 ctttacccgg tggtgcatat cggggatgaa agctggcgca tgatgaccac cgatatggcc
    12781 agtgtgccgg tctccgttat cggggaagaa gtggctgatc tcagccaccg cgaaaatgac
    12841 atcaaaaacg ccattaacct gatgttctgg ggaatataaa tgtcaggctc cgttatacac
    12901 agccagtctg cagtcatggt accggcagtg tattctgccg ggcagcctgc atcgctgcct
    12961 gttgccattg attatccggc agctctggca cttcgccaga tgtcgatggt ccatgatgaa
    13021 ctgccaaaat atctgctggc tccggaagtg agtgccctgc tccattacgt cccggatctg
    13081 caccgcaaga tgctgctggc cacactgtgg aacaccggag cacgcattaa tgaagcactg
    13141 gcgctgacgc ggggggattt ttcgcttgcg cctccgtatc cgtttgtgca gcttgcgacc
    13201 ctgaaacaac ggaccgaaaa agctgccagg acggcgggga gaatgcccgc cggtcagcag
    13261 actcaccggc tggttccgct ctctgactcc tggtacgtca gccagctgca gacgatggtg
    13321 gcaacactga aaatacccat ggagcggcgt aaccgtcgca caggaaggac agagaaagcg
    13381 cggatctggg aagtgacgga cagaacggtc aggacctgga ttggggaggc ggttgccgcc
    13441 gctgctgctg acggtgtgac gttctctgtt ccggtcacac cacatacgtt ccgccattcc
    13501 tatgcgatgc acatgctgta tgccggtata ccgctgaaag ttctgcaaag cctgatggga
    13561 cataagtcca tcagttcaac ggaagtctac acgaaggttt ttgcgctgga tgtggctgcc
    13621 cggcaccggg tgcagtttgc gatgccggag tctgatgcgg ttgcgatgct gaaacaatta
    13681 tcctgagaat aaatgccttg gcctttatat ggaaatgtgg aactgagtgg atatgctgtt
    13741 tttgtctgtt aaacagagaa gctggctgtt atccactgag aagcgaacga aacagtcggg
    13801 aaaatctccc attatcgtag agatccgcat tattaatctc aggagcctgt gtagcgttta
    13861 taggaagtag tgttctgtca tgatgcctgc aagcggtaac gaaaacgatt tgaatatgcc
    13921 ttcaggaaca atagaaatct tcgtgcggtg ttacgttgaa gtggagcgga ttatgtcagc
    13981 aatggacaga acaacctaat gaacacagca ccatgatgtg gtctgtcctt ttacagccag
    14041 tagtgctcgc cgcagtcgag cgacagggcg aagccctcga gtgagcgagg aagcaccagg
    14101 gaacagcact tatatattct gcttacacac gatgcctgaa aaaacttccc ttggggttat
    14161 ccacttatcc acggggatat ttttataatt atttttttta tagtttttag atcttctttt
    14221 ttagagcgcc ttgtaggcct ttatccatgc tggttctaga gaaggtgttg tgacaaattg
    14281 ccctttcagt gtgacaaatt accctcaaat gacagtcctg tctgtgacaa attgccctta
    14341 accctgtgac agattgccct cagaagaagc tgttttttca caaagttatc cctgcttatt
    14401 gactcttttt tatttagtgt gacaatctaa aaacttgtca cacttcacat ggatctgtca
    14461 tggcggaaac agcggttatc aatcacaaga aacgtaaaaa tagcccgcga atcgtccagt
    14521 caaacgacct cactgaggcg gcatatagtc tctcccggga tcaaaaacgt atgctgtatc
    14581 tgttcgttga ccagatcaga aaatctgatg gcaccctaca ggaacatgac ggtatctgcg
    14641 agatccatgt tgctaaatat gctgaaatat tcggattgac ctctgcggaa gccagtaagg
    14701 atatacggca ggcattgaag agtttcgcgg ggaaggaagt ggttttttat cgccctgaag
    14761 aggatgccgg cgatgaaaaa ggctatgaat cttttccctg gtttatcaaa cgtgcgcaca
    14821 gtccatccag agggctttac agtgtacata tcaacccata tctcattccc ttctttatcg
    14881 ggttacagaa ccggtttacg cagtttcggc ttagtgaaac aaaagaaatc accaatccgt
    14941 atgccatgcg tttatacgaa tccctgtgtc agtatcgtaa gccggatggc tcaggcatcg
    15001 tctctctgaa aatcgactgg atcatagagc gttaccagct gcctcaaagt taccagcgta
    15061 tgcctgactt ccgccgccgc ttcctgcagg tctgtgttaa tgagatcaac agcagaactc
    15121 caatgcgcct ctcatacatt gagaaaaaga aaggccgcca gacgactcat atcgtatttt
    15181 ccttccgcga tatcacttcc atgacgacag gatagtctga gggttatctg tcacagattt
    15241 gagggtggtt cgtcacattt gttctgacct actgagggta atttgtcaca gttttgctgt
    15301 ttccttcagc ctgcatggat tttctcatac tttttgaact gtaattttta aggaagccaa
    15361 atttgagggc agtttgtcac agttgatttc cttctctttc ccttcgtcat gtgacctgat
    15421 atcgggggtt agttcgtcat cattgatgag ggttgattat cacagtttat tactctgaat
    15481 tggctatccg cgtgtgtacc tctacctgga gtttttccca cggtggatat ttcttcttgc
    15541 gctgagcgta agagctatct gacagaacag ttcttctttg cttcctcgcc agttcgctcg
    15601 ctatgctcgg ttacacggct gcggcgagcg ctagtgataa taagtgactg aggtatgtgc
    15661 tcttcttatc tccttttgta gtgttgctct tattttaaac aactttgcgg ttttttgatg
    15721 actttgcgat tttgttgttg ctttgcagta aattgcaaga tttaataaaa aaacgcaaag
    15781 caatgattaa aggatgttca gaatgaaact catggaaaca cttaaccagt gcataaacgc
    15841 tggtcatgaa atgacgaagg ctatcgccat tgcacagttt aatgatgaca gcccggaagc
    15901 gaggaaaata acccggcgct ggagaatagg tgaagcagcg gatttagttg gggtttcttc
    15961 tcaggctatc agagatgccg agaaagcagg gcgactaccg cacccggata tggaaattcg
    16021 aggacgggtt gagcaacgtg ttggttatac aattgaacaa attaatcata tgcgtgatgt
    16081 gtttggtacg cgattgcgac gtgctgaaga cgtatttcca ccggtgatcg gggttgctgc
    16141 ccataaaggt ggcgtttaca aaacctcagt ttctgttcat cttgctcagg atctggctct
    16201 gaaggggcta cgtgttttgc tcgtggaagg taacgacccc cagggaacag cctcaatgta
    16261 tcacggatgg gtaccagatc ttcatattca tgcagaagac actctcctgc ctttctatct
    16321 tggggaaaag gacgatgtca cttatgcaat aaagcccact tgctggcctg ggcttgacat
    16381 tattccttcc tgtctggctc tgcaccgtat tgaaactgag ttaatgggca aatttgatga
    16441 aggtaaactg cccaccgatc cacacctgat gctccgactg gccattgaaa ctgttgctca
    16501 tgactatgat gtcatagtta ttgacagcgc gcctaacctg ggtatcggca cgattaatgt
    16561 cgtatgtgct gctgatgtgc tgattgttcc cacgcctgct gagttgtttg actacacctc
    16621 cgcactgcag tttttcgata tgcttcgtga tctgctcaag aacgttgatc ttaaagggtt
    16681 cgagcctgat gtacgtattt tgcttaccaa atacagcaat agtaatggct ctcagtcccc
    16741 gtggatggag gagcaaattc gggatgcctg gggaagcatg gttctaaaaa atgttgtacg
    16801 tgaaacggat gaagttggta aaggtcagat ccggatgaga actgtttttg aacaggccat
    16861 tgatcaacgc tcttcaactg gtgcctggag aaatgctctt tctatttggg aacctgtctg
    16921 caatgaaatt ttcgatcgtc tgattaaacc acgctgggag attagataat gaagcgtgcg
    16981 cctgttattc caaaacatac gctcaatact caaccggttg gagatacttc gttatcgaca
    17041 ccagctgccc cgatggtgga ttcgttaatt gcgcgcgtag gagtaatggc tcgcggtaat
    17101 gccattactt tgcctgtatg tggtcgggat gtgaagttta ctcttgaagt gctccggggt
    17161 gatagtgttg agaagacctc tcgggtatgg tcaggtaatg aacgtgacca ggagctgctt
    17221 actgaggact cactggatga tctcatccct tcttttctac tgactggtca acagacaccg
    17281 gcgttcggtc gaagagtatc tggtgtcata gaaattgccg atgggagtcg ccgtcgtaaa
    17341 gctgctgcac ttaccgaaag tgattatcgt gttctggttg gcgagctgga tgatgagcag
    17401 atggctgcat tatccagatt gggtaacgat tatcgcccaa caagtgctta tgaacgtggt
    17461 cagcgttatg caagccgatt gcagaatgaa tttgctggaa atatttctgc gctggctgat
    17521 gcggaaaata tttcacgtaa gattattacc cgctgtatca acacagccaa attgcctaaa
    17581 tcagttgttg ctcttttttc tcaccccggt gaactatctg cccggtcagg tgatgcactt
    17641 caaaaagcct ttacagataa agaggaatta cttaagcagc aggcatctaa ccttcatgag
    17701 cagaaaaaag ctggggtgat atttgaagct gaagaagtta tcactctttt aacttctgtg
    17761 cttaaaacgt catctgcatc aagaactagt ttaagctcac gacatcagtt tgctcctgga
    17821 gcgacagtat tgtataaggg cgataaaatg gtgcttaacc tggacaggtc tcgtgttcca
    17881 actgagtgta tagagaaaat tgaggccatt cttaaggaac ttgaaaagcc agcaccctga
    17941 tgcgaccacg ttttagtcta cgtttatctg tctttactta atgtcctttg ttacaggcca
    18001 gaaagcataa ctggcctgaa tattctctct gggcccactg ttccacttgt atcgtcggtc
//
LOCUS       DAMZFT010000097        17887 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.097, whole genome shotgun sequence.
ACCESSION   DAMZFT010000097 DAMZFT010000000
VERSION     DAMZFT010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17887)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17887)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17887
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.097"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(<1..173)
                     /locus_tag="PN977_003470"
     CDS             complement(<1..173)
                     /locus_tag="PN977_003470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HDI6043472.1"
                     /translation="MKTQRGYTLIETLVAMLILVMLSASGLYGWQYWQQSQRLWQTAS
                     QARDYLLYLREDA"
     gene            complement(358..1152)
                     /gene="thyA"
                     /locus_tag="PN977_003471"
     CDS             complement(358..1152)
                     /gene="thyA"
                     /locus_tag="PN977_003471"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HDI6043473.1"
                     /translation="MKQYLELMQKVLDEGTQKNDRTGTGTLSIFGHQMRFNLQDGFPL
                     VTTKRCHLRSIIHELLWFLQGDTNIAYLHENNVTIWDEWADENGDLGPVYGKQWRAWP
                     TPDGRHIDQITTVLNQLKNDPDSRRIIVSAWNVGELDKMALAPCHAFFQFYVADGKLS
                     CQLYQRSCDVFLGLPFNIASYALLVHMMAQQCDLEVGDFVWTGGDTHLYSNHMDQTHL
                     QLSREPRPLPKLIIKRKPESIFDYRFEDFEIEGYDPHPGIKAPVAI"
     gene            complement(1159..2034)
                     /gene="lgt"
                     /locus_tag="PN977_003472"
     CDS             complement(1159..2034)
                     /gene="lgt"
                     /locus_tag="PN977_003472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HDI6043474.1"
                     /translation="MTSSYLHFPEFDPVIFSIGPVALHWYGLMYLVGFIFAMWLATRR
                     ANRPGSGWTKNEVENLLYAGFLGVFLGGRIGYVLFYNFPQFMADPLYLFRVWDGGMSF
                     HGGLIGVIVVMIIFARRTKRSFFQVSDFIAPLIPFGLGAGRLGNFINGELWGRVDPNF
                     PFAMLFPGSRTEDILLLQTNPQWQSIFDTYGVLPRHPSQLYELLLEGVVLFIILNLYI
                     RKPRPMGAVSGLFLIGYGAFRIIVEFFRQPDAQFTGAWVQYISMGQILSIPMIVAGVI
                     MMVWAYRRSPQQHVS"
     gene            complement(2185..4431)
                     /gene="ptsP"
                     /locus_tag="PN977_003473"
     CDS             complement(2185..4431)
                     /gene="ptsP"
                     /locus_tag="PN977_003473"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HDI6043475.1"
                     /translation="MLTRLREIVEKVASAPRLNEALNILVTDICLAMDTEVCSVYLAD
                     HDRRCYYLMATRGLKKPRGRTVTLAFDEGIVGLVGRLAEPINLADAQKHPSFKYVPSV
                     KEERFRAFLGVPIIQRRQLLGVLVVQQRELRQYDESEESFLVTLATQMAAILSQSQLT
                     ALFGQYRQTRIRALPAAPGVAIAEGWQDATLPLMEQVYQASTLDPALERERLTGALEE
                     AANEFRRYSKRFAAGAQKETAAIFDLYSHLLSDTRLRRELFAEVDKGSVAEWAVKTVI
                     EKFAEQFAALSDNYLKERAGDLRALGQRLLFHLDDANQGPNAWPERFILVADELSATT
                     LAELPQDRLVGVVVRDGAANSHAAIMVRALGIPTVMGADIQPSVLHRRTLIVDGYRGE
                     LLVDPEPVLLQEYQRLISEEIELSRLAEDDVNLPAQLKSGERIKVMLNAGLSPEHEEK
                     LGSRIDGIGLYRTEIPFMLQSGFPSEEEQVAQYQGMLQMFNDKPVTLRTLDVGADKQL
                     PYMPISEENPCLGWRGIRITLDQPEIFLIQVRAMLRANAATGNLNILLPMVTSLDEVD
                     EARRLIERAGREVEEMIGYEIPKPRIGIMLEVPSMVFMLPHLAKRVDFISVGTNDLTQ
                     YILAVDRNNTRVANIYDSLHPAMLRALAMIAREAEIHGIDLRLCGEMAGDPMCVAILI
                     GLGYRHLSMNGRSVARVKYLLRRIDFAEAENLAQRSLEAQLATEVRHQVAAFMERRGM
                     GGLIRGGL"
     gene            complement(4444..4974)
                     /gene="rppH"
                     /locus_tag="PN977_003474"
     CDS             complement(4444..4974)
                     /gene="rppH"
                     /locus_tag="PN977_003474"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001655775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HDI6043476.1"
                     /translation="MIDDDGYRPNVGIVICNRQGQVMWARRFGQHSWQFPQGGINPGE
                     SAEQAMYRELFEEVGLSRKDVRILASTRNWLRYKLPKRLVRWDTKPVCIGQKQKWFLL
                     QLVSGDAEINMQTSSTPEFDGWRWVSYWYPVRQVVSFKRDVYRRVMKEFASVVMSLQE
                     NTPKPQNASAYRRKRG"
     gene            5659..6348
                     /gene="mutH"
                     /locus_tag="PN977_003475"
     CDS             5659..6348
                     /gene="mutH"
                     /locus_tag="PN977_003475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutH"
                     /protein_id="HDI6043477.1"
                     /translation="MSQPRPLLSPPETEEQLLAQAQQLSGYTLGELAALAGLVTPENL
                     KRDKGWIGVLLEIWLGASAGSKPEQDFAALGVELKTIPVDSLGRPLETTFVCVAPLTG
                     NSGVTWETSHVRHKLKRVLWIPVEGERSIPLAQRRVGSPLLWSPNEEEDRQLREDWEE
                     LMDMIVLGQVERITARHGEYLQIRPKAANAKALTEAIGVRGERILTLPRGFYLKKNFT
                     SALLARHFLIQ"
     gene            6417..7130
                     /locus_tag="PN977_003476"
     CDS             6417..7130
                     /locus_tag="PN977_003476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HDI6043478.1"
                     /translation="MLFAWITDPNAWLALGTLTLLEIVLGIDNIIFLSLVVAKLPTAQ
                     RAHARRLGLAGAMVMRLALLASIAWVTRLTNPLFTIFSQEISARDLILLLGGLFLIWK
                     ASKEIHESIEGEEEGLKTRVSSFLGAIVQIMLLDIIFSLDSVITAVGLSDHLFIMMAA
                     VVIAVGVMMFAARSIGDFVERHPSVKMLALSFLILVGFTLILESFDIHVPKGYIYFAM
                     FFSIAVESLNLIRNKKNPL"
     gene            7268..7486
                     /gene="ygdR"
                     /locus_tag="PN977_003477"
     CDS             7268..7486
                     /gene="ygdR"
                     /locus_tag="PN977_003477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YgdR"
                     /protein_id="HDI6043479.1"
                     /translation="MKKWAVIISAVGLAFAVSGCSSDYVMATKDGRMILTDGKPEIDD
                     DTGLVSYHDQQGNAMQINRDDVSQIIER"
     gene            7594..8634
                     /locus_tag="PN977_003478"
     CDS             7594..8634
                     /locus_tag="PN977_003478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(H)-dependent aldo-keto reductase"
                     /protein_id="HDI6043480.1"
                     /translation="MQYHRIPHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGI
                     NLIDVAEMYPVPPRPETQGLTETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPD
                     QALDRKNIREALHDSLKRLQTDYLDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDT
                     LDALAEYQRAGKIRYIGVSNETAFGVMRYLHLADKHDLPRIVTIQNPYSLLNRSFEVG
                     LAEVSQYEGVELLAYSCLGFGTLTGKYLNGAKPAGARNTLFSRFTRYSGEQTQKAVAA
                     YVDIARRHGLDPAQMALAFVRRQPFVASTLLGATTMEQLKTNVESLHLELSEDVLAEI
                     EAVHQVYTYPAP"
     gene            8822..10447
                     /locus_tag="PN977_003479"
     CDS             8822..10447
                     /locus_tag="PN977_003479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002432455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4139 domain-containing protein"
                     /protein_id="HDI6043481.1"
                     /translation="MLLTGCLSSAWGAQLTQALTLKDVTVYLHGASLQGEETLNLPAG
                     ESEIIFTNIANSVTDDSIAISIDNGVTVLSSRIALMETQPNALPSDTQQQLKAVKVEQ
                     AKLTAEKNNIAAQIKVLEANSTLTNKSAAEVGKYLQMVKEKNGELLRADNELTQKLAD
                     NQLGIEKLEQRESMNNGDSEASDDPQVIIAKVYVPKAVTAKAVVHYITPDATWTPIYD
                     IHATGINQPLRLVYKANIRQYTGLPWKNVNLVLSTSEPDTHLTLPHLEPLYVDVTEYG
                     GVSGSLSATPVMKQRSEYSSDQDASELQKSLEAEMQKSRERPAGRKYAAQAAPASARP
                     ESFTAQNVRYALSLPWQIDSDNTARTVVIKEESVSGEYRFFAIPKISNDAYLQVQIKE
                     WEQLNLIPGESQIFFGNNRTGVGYLQPPEDGKSLDIQLNRDPKLIIQRKAVLKKENAL
                     SIFNDSAVRNFSYTLNVKNSYGDAVDITLLDQIPVSANSDITVEKMQYGQAQFDAKTG
                     ELSWTLKLSGKENVSLPFSYSVKYPKGMHPSGM"
     gene            complement(10486..11679)
                     /gene="lplT"
                     /locus_tag="PN977_003480"
     CDS             complement(10486..11679)
                     /gene="lplT"
                     /locus_tag="PN977_003480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid transporter LplT"
                     /protein_id="HDI6043482.1"
                     /translation="MSESVHTNTSLWSKGMKAVIVAQFLSAFGDNALLFATLALLKAQ
                     FYPEWSQPILQMVFVGAYILFAPFVGQVADSFAKGRVMMFANGLKLLGAASICFGINP
                     FLGYTLVGVGAAAYSPAKYGILGELTTGSKLVKANGLMEASTIAAILLGSVAGGVLAD
                     WHVLVALAACALAYGGAVVANIYIPKLAAARPGQSWNLINMTRSFLNACTSLWRNGET
                     RFSLVGTSLFWGAGVTLRFLLVLWVPVALGITDNATPTYLNAMVAIGIVVGAGAAAKL
                     VTLETVSRCMPAGILIGVVVLIFSLQHELLPAYALLMLIGVLGGFFVVPLNALLQERG
                     KKSVGAGNAIAVQNLGENSAMLLMLGIYSLAVMVGIPVVPIGIGFGALFALAITALWI
                     WQRRH"
     gene            complement(11672..13831)
                     /gene="aas"
                     /locus_tag="PN977_003481"
     CDS             complement(11672..13831)
                     /gene="aas"
                     /locus_tag="PN977_003481"
                     /EC_number="2.3.1.40"
                     /EC_number="6.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acyl-ACP--phospholipid
                     O-acyltransferase/long-chain-fatty-acid--ACP ligase"
                     /protein_id="HDI6043483.1"
                     /translation="MLFSFFRNVCRVLYRVRVTGDNQALKGERVLITPNHVSFIDGIL
                     LALFLPVRPVFAVYTSISQQWYMRWLKSFIDFVPLDPTQPMAIKHLVRLVEQGRPVVI
                     FPEGRITTTGSLMKIYDGTGFVAAKSGATVIPVRIEGAELTHFSRLKGLVKRRLFPQI
                     TLHILPPTQVEMPDAPRARDRRKIAGEMLHQIMMEARMAVRPRETLYESLLSAMYRFG
                     AGKKCVEDVNFTPDSYRKLLTKTLFVGRILEKYSVEGERIGLMLPNAGISAAVIFGAI
                     ARRRIPAMMNYTAGVKGLTSAITAAEIKTIFTSRQFLDKGKLWHLPEQLTQVRWVYLE
                     DLKADVTTADKVWIFAHLLMPRLAQVKQQPEEEALILFTSGSEGHPKGVVHSHKSILA
                     NVEQIKTIADFTTNDRFMSALPLFHSFGLTVGLFTPLLTGAEVFLYPSPLHYRIVPEL
                     VYDRSCTVLFGTSTFLGHYARFANPYDFYRLRYVVAGAEKLQESTKQLWQDKFGLRIL
                     EGYGVTECAPVVSINVPMAAKPGTVGRILPGMDARLLSVPGIEEGGRLQLKGPNIMNG
                     YLRVEKPGVLEVPTAENVRGEMERGWYDTGDIVRFDEQGFVQIQGRAKRFAKIAGEMV
                     SLEMVEQLALGVSPDKVHATAIKSDASKGEALVLFTTDNELTRDKLQQYAREHGVPEL
                     AVPRDIRYLKQMPLLGSGKPDFVTLKSWVDEAEQHDE"
     gene            14417..15448
                     /gene="galR"
                     /locus_tag="PN977_003482"
     CDS             14417..15448
                     /gene="galR"
                     /locus_tag="PN977_003482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalR"
                     /protein_id="HDI6043484.1"
                     /translation="MATIKDVARLAGVSVATVSRVINNSPKASEASRLAVHSAMESLS
                     YHPNANARALAQQTTETVGLVVGDVSDPFFGAMVKAVEQVAYHTGNFLLIGNGYHNEQ
                     KERQAIEQLIRHRCAALVVHAKMIPDADLASLMKQMPGMVLINRILPGFENRCIALDD
                     RYGAWLATRHLIQQGHTRIGYLCSNHSISDAEDRLQGYYDALAESGIPTNDRLVTFGE
                     PDESGGEQAMTELLGRGRNFTAVACYNDSMAAGAMGVLNDNGIDVPGEISLIGFDDVL
                     VSRYVRPRLTTVRYPIVTMATQAAELALALADNRPLPEITNVFSPTLVRRHSVSTPSL
                     EASHHATSD"
     gene            complement(15455..16717)
                     /gene="lysA"
                     /locus_tag="PN977_003483"
     CDS             complement(15455..16717)
                     /gene="lysA"
                     /locus_tag="PN977_003483"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HDI6043485.1"
                     /translation="MPHSLFSTDTDLTAENLLRLPAEFGCPVWVYDAQIIRRQIAALK
                     QFDVVRFAQKACSNIHILRLMREQGVKVDSVSLGEIERALAAGYNPQTHPDDIVFTAD
                     VIDQATLERVSELQIPVNAGSVDMLDQLGQVSPGHRVWLRVNPGFGHGHSQKTNTGGE
                     NSKHGIWYTDLPAALDVIQRHHLQLVGIHMHIGSGVDYAHLEQVCGAMVRQVLEFGQD
                     LQAISAGGGLSIPYQQGEEAVDTEHYYGLWNAAREQIARHLGHPVKLEIEPGRFLVAQ
                     AGVLITQVRSVKQMGSRHFVLVDAGFNDLMRPAMYGSYHHISALTADGRSLEYAPTVE
                     TVVAGPLCESGDVFTQQEGGNVETRVLPEVKAGDYLVLHDTGAYGASMSSNYNSRPLL
                     PEVLFDNGQARLIRRRQTIEELLALELL"
     gene            16839..17774
                     /gene="lysR"
                     /locus_tag="PN977_003484"
     CDS             16839..17774
                     /gene="lysR"
                     /locus_tag="PN977_003484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator LysR"
                     /protein_id="HDI6043486.1"
                     /translation="MAAVNLRHIEIFHAVMTAGSLTEAAHLLHTSQPTVSRELARFEK
                     VIGLKLFERIRGRLHPTVQGLRLFEEVQRSWYGLDRIVSAAESLREFRQGELSIACLP
                     VFSQSFLPQLLQPFLARYPDVSLNIVPQESPLLEEWLSAQRHDLGLTETLHTPAGTER
                     TELLSLDEVCVLPPGHPLAVKKVLTPDDFHGENYISLSRTDSYRQFLDQLFTENQVKR
                     RMIVETHSAASVCAMVRAGVGVSVVNPLTALDYAASGLVVRRFSIAVPFTVSLIRPLH
                     RPSSALVQAFSEHLQAGLPKLVTSLDAILSSATTA"
     gene            complement(17761..>17887)
                     /locus_tag="PN977_003485"
     CDS             complement(17761..>17887)
                     /locus_tag="PN977_003485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aspartate/glutamate racemase"
                     /protein_id="HDI6043487.1"
                     /translation="AQGVIFGCTEIGLLVPEERSVLPVFDTAAIHAEDAVAFMLS"
BASE COUNT         4511 a         4717 c         4466 g         4193 t
ORIGIN      
        1 ttggcatctt cacgtaaata gagcaaatag tcccgcgcct ggctggcggt ttgccaaagc
       61 cgttgcgact gctgccaata ttgccagcca tagagcccac ttgcgcttag catgaccaga
      121 atcagcatcg cgaccagcgt ttcaatcagc gtataaccac gttgtgtttt catgccggca
      181 gtatggagcg aggagaaaaa aagacgaggg ccagtttcta tttcttcggc gcatcttccg
      241 gactatttac gccgttgcag gacattgcaa aatttcggga aggcgtctcg aagaatttaa
      301 cggagggtaa aaaaaaccga cgcacactgg cgtcggctct ggcaggatgt ttcgtaatta
      361 gatagccacc ggcgctttaa tgcccggatg tggatcgtag ccttcaatct caaagtcttc
      421 gaaacggtag tcgaagatgg attcgggttt acgtttgata atcaacttcg gcagcggacg
      481 cggttcgcgg cttaattgca gatgagtttg atccatatgg ttgctgtaca ggtgcgtgtc
      541 gccaccggtc cagacaaaat cacccacttc cagatcgcac tgctgcgcca tcatatgcac
      601 cagtaacgcg tagctggcaa tgttgaacgg caggccgagg aagacgtcac aggagcgctg
      661 ataaagctgg caagagagtt tgccgtctgc cacatagaac tggaagaatg catggcacgg
      721 tgccagcgcc attttatcca gttcgcctac gttccacgct gaaacaataa tgcggcgcga
      781 atccgggtcg tttttcagct ggttcagtac cgtagtgatc tggtcaatat gacgaccatc
      841 tggcgttggc caggcgcgcc actgtttacc atacactggc ccgaggtcgc cgttttcatc
      901 ggcccattcg tcccagatgg tgacattgtt ttcgtgtaga taagcaatgt tagtgtcgcc
      961 ctgcaggaac cacagcagtt cgtggatgat ggaacgcagg tggcaacgtt tggttgtcac
     1021 cagcgggaat ccatcctgca ggttaaaacg catctgatga ccaaaaatgg aaagcgttcc
     1081 ggttccggta cggtcgtttt tctgtgtgcc ttcgtcgagc actttttgca tcagttctaa
     1141 atactgtttc atggttcctc aggaaacgtg ttgctgtggg ctgcgacgat atgcccagac
     1201 catcatgatc acacccgcga caatcatcgg gatggaaaga atttgcccca tgctaatgta
     1261 ctgcacccag gcaccggtaa actgcgcgtc gggctggcgg aaaaactcaa caatgatgcg
     1321 aaacgcgccg taaccaatca ggaacaaacc tgagacagct cccattgggc gcggtttacg
     1381 aatatacagg ttgaggataa taaacagcac cacaccttcc agcagcagct cgtaaagctg
     1441 tgatgggtgg cgcggcagca caccgtaagt gtcgaaaatg gattgccact gcgggttggt
     1501 ttgcagcagc aaaatatctt ctgtacggga gccagggaac agcatggcaa acgggaagtt
     1561 tgggtcaacg cggccccaca attcaccgtt aataaagttg cccagacgcc cggcaccaag
     1621 accaaacgga atgagtgggg cgataaaatc agaaacctgg aagaaggaac gtttagtacg
     1681 gcgggcgaag ataatcatca ccacgataac gccaatcagg ccgccgtgga aggacatgcc
     1741 gccgtcccag acacggaaca gatacagcgg atcggccata aactgcggga aattgtagaa
     1801 cagaacataa ccaatacgtc caccgaggaa gacgccgagg aagcccgcat agagtaagtt
     1861 ttcaacttca tttttggtcc agccgctgcc cggacgattc gcccgacgtg ttgccagcca
     1921 cattgcaaaa atgaaaccca ccagatacat caggccgtac cagtgaagcg ctacgggtcc
     1981 tattgagaaa atgaccggat caaactccgg aaaatgcaga tagctactgg tcatctgtca
     2041 ccacaagttc ttgttatttc gctgaaagag aacagcgatt gaaatgcgcg ccgcaggttt
     2101 caggcgctcc aaaggtgcga ataatagcac aaggggacca ggctggttgc cggataccgt
     2161 taaaagatat gtatatgatc cgcgctataa ccctccgcga atcagcccgc ccatgccgcg
     2221 acgctccata aaggctgcaa cctgatggcg aacttcggtc gccagttgcg cttccagact
     2281 acgctgcgca agattttctg cttcagcaaa atcaatgcgc cgcagcaggt attttacgcg
     2341 cgctacagaa cgtccgttca tagacagatg gcgataccca agcccaatga ggattgccac
     2401 gcacatggga tcgcccgcca tttcaccgca caaacggaga tcgattccat gtatttccgc
     2461 ttcccgggcg atcatcgcca gagctcgtaa cattgcagga tgaagactgt cataaatgtt
     2521 cgccacccgg gtattgttgc gatcaacagc caggatgtat tgcgtcagat cgttggtgcc
     2581 aacagagatg aaatcgaccc gctttgccag atgcggcagc ataaatacca ttgacggcac
     2641 ttccagcatg atgccgatac gtggtttggg aatttcgtaa ccgatcatct cctcgacttc
     2701 acgtccggca cgttcaatca ggcggcgtgc ttcgtcaact tcatcgaggc ttgtgaccat
     2761 cggcaacaga atattcaggt tgcccgtagc ggcattagca cgcagcatcg cccgcacctg
     2821 gatcaagaag atctccggct gatcgagcgt aatgcgaatc ccacgccaac ccaggcatgg
     2881 attctcttcg ctgatcggca tgtaaggcag ctgcttatct gctccgacat ccagcgtacg
     2941 caaggtgacg ggtttatcat tgaacatttg cagcatcccc tgatactgcg ccacctgttc
     3001 ttcttccgac ggaaaaccac tttgcagcat gaatgggatt tcagtgcgat aaagtcctat
     3061 gccatcaata cggctgccca gtttttcttc atgttccggg cttaaaccgg cattgagcat
     3121 gactttaatg cgttcgccgc tttttaactg ggcgggtaaa ttgacgtcat cttccgccag
     3181 acggctaagc tcgatctctt cactaattag ccgctgatat tcttgcagca gtaccggctc
     3241 cggatcgacc agcaattcac cgcgataacc atcgacgatc agcgtccgac gatgcagtac
     3301 cgaaggctga atatccgcgc ccatcacggt aggtatcccc agcgcacgta ccatgatcgc
     3361 agcatgggag ttggcagcgc catcgcgcac gacaacaccg actaagcgat cctggggcag
     3421 ctcagcaagc gttgtcgctg acagttcatc tgccaccaga atgaaacgtt ccggccaggc
     3481 gttcggccct tgattagcgt catcaagatg aaacagcaat cgctggccca gcgcacgtaa
     3541 atcgccagcc cgctctttga gatagttatc gcttagcgcg gcaaactgtt cggcaaattt
     3601 ttcaatgacc gtttttaccg cccactctgc caccgagcct ttatcaacct cggcaaacaa
     3661 ttcgcgacgc agccgggtgt ccgaaagcag gtgcgagtaa agatcgaaaa tagccgccgt
     3721 ttctttttgt gcaccggcgg caaagcgttt gctgtagcgg cgaaactcgt ttgccgcttc
     3781 ttccagcgcc ccggtcagtc gttcgcgttc cagagctgga tccagcgttg atgcctgata
     3841 cacctgttcc attaaaggta acgtggcatc ctgccagcct tcggcaatcg ccacaccagg
     3901 tgctgccggt aatgcgcgga ttcgcgtctg gcgatattgt ccaaacaagg cagtcaactg
     3961 cgactgagaa agaatagctg ccatctgggt ggcaagcgtc accaggaagg attcttcact
     4021 ttcgtcatac tggcgcaact ctcgttgctg taccaccagt acaccaagca actggcgacg
     4081 ttgaataatt ggtacgccta aaaatgcgcg gaaacgttct tcttttacgg aggggacgta
     4141 tttgaagctg gggtgctttt gcgcatctgc aaggtttatc ggttccgcca gcctgccaac
     4201 caggccgacg atcccttcat caaacgcgag cgttacagtg cgaccgcgtg gttttttcag
     4261 cccccgggtc gccatcaggt agtaacaacg tcgatcatga tcggcaaggt aaaccgaaca
     4321 gacctcggta tccatcgcaa gacagatgtc ggtaaccaga atatttaacg cctcattcag
     4381 gcgtggtgcg ctggctacct tttcgactat ttcgcgcagg cgagtgagca taattggcgt
     4441 gacttaacct cttttacgtc gataagcaga tgcgttttgt ggttttggcg tattttcctg
     4501 cagtgacatc accacactcg cgaactcttt cattactcta cggtagacat cacgtttaaa
     4561 tgacaccacc tgtctgaccg gataccagta acttacccat cgccagccgt caaactctgg
     4621 tgtactgctg gtttgcatat tgatttctgc atcgccgctc accagctgca agagaaacca
     4681 tttttgtttt tggccgatac aaaccggctt cgtgtcccaa cgcaccaaac gtttcggtaa
     4741 tttgtagcgc aaccagttac gcgttgaagc aaggattcga acgtctttac ggcttaatcc
     4801 tacttcttca aacaattcac ggtacatcgc ctgctctgcg gattctccgg ggttgattcc
     4861 gccttgcgga aattgccagg agtgctgacc aaatcgccgg gcccacatta cctgcccctg
     4921 gcgattacaa atcacgatac ctacgtttgg gcggtagcca tcgtcatcaa tcaccggact
     4981 acctcaaaat aaagctttat atacgaatga ttgtttcata ctccaggaag acggtaaacc
     5041 actctctgca gggcattaca cactaataac aattgaataa ctcacagtta tgtgcagagt
     5101 tataaacaga ggaaggggtg gatagccgtt tttattcacc ttttctgtgg atagagttgt
     5161 gaagaactac ggaattacta cgggaaaacc cggagcattc tgaataagcc cgatatggac
     5221 attttaaaat attcattatc agtcaatgca ttacatgtaa ttttagtaat aaaaaagagt
     5281 aattcgtgac ccaggtcaca cctctcattt acgggttggc gaaagatcaa ccaatgccgt
     5341 atttatccac agaatgtgcc actaagttaa gcactgaacc actaaaaact ggagtttcgt
     5401 cgcacgtcaa ggctgtaaat ggaaacagta gtggaggttt ttcacagtta tcccagcttt
     5461 ctgtggataa catggtgtaa gatcctgttt attttcagtg accagatttg gaaaacccat
     5521 tgcagtgttg cgcaactcga ttaccggcaa cctaaaaagc aatataaatc agagaattga
     5581 acaacgcctg tggaaaaagt tacactgcga atattcggca cataattgct gtttgttttt
     5641 taaccaaggt atcgtgacat gtcccaacct cgcccactgc tctctcctcc cgaaactgaa
     5701 gaacaattgt tagcgcaagc acaacaactt tctggttata cattgggaga actggcggca
     5761 cttgctgggc tggtgacgcc ggagaattta aaacgcgata agggctggat tggcgtgtta
     5821 ctggagatct ggctaggtgc cagcgcaggg agtaaacctg agcaagattt tgctgctctg
     5881 ggcgtggaac ttaaaactat ccctgtggat agtcttggtc gtccgctgga aacaacattc
     5941 gtttgtgttg ccccgttaac gggtaatagc ggggtgacct gggaaaccag ccacgtgcgc
     6001 cacaagctca aacgcgtact gtggataccg gttgaaggcg agcgcagcat cccgctggcg
     6061 cagcgtcgcg taggatcacc gttactgtgg agcccgaatg aagaggaaga ccggcaactg
     6121 cgcgaagact gggaagaatt aatggatatg attgttctcg gtcaggttga gcggatcacc
     6181 gctcgtcacg gggaatattt acagatacga ccgaaagcag cgaatgcgaa agcgcttacc
     6241 gaagccattg gtgtccgggg cgaacggatt ctgacgctgc cgcgcggctt ttatttgaag
     6301 aagaatttca ccagtgcgct actggcccgt cattttctga tccagtagcc atcgctttga
     6361 cctgccgctt tccgggcata taattaccgc ttcatttttt tggcagggct ttttagatgt
     6421 tatttgcatg gataaccgat cctaacgcct ggcttgcgct cggtacgctg acgctgctgg
     6481 agatcgttct tgggatcgac aatattattt tcctttctct ggtggtggca aagctcccca
     6541 cagcacaacg tgctcatgcg cgccgtctgg ggttggcggg agccatggtt atgcgtctgg
     6601 cgctgctggc atccatcgcc tgggttacgc gcctgacaaa tccgcttttt acaatattca
     6661 gccaggaaat ttccgcccgt gatttgattc tgcttctggg tggcttgttc cttatctgga
     6721 aagccagcaa ggaaatccac gaatccattg aaggtgaaga agaagggctg aaaacacgcg
     6781 tttcatcatt cctcggcgct atcgtgcaga ttatgctgct ggatattatc ttcagcctcg
     6841 actcggtgat taccgctgtg ggtctgtcag atcacctgtt tattatgatg gccgccgtgg
     6901 tgattgccgt aggcgtgatg atgttcgccg cacgctcgat tggtgatttt gtcgaacgcc
     6961 atccttcggt aaaaatgctg gcgctctctt tcctgattct ggtgggcttt accttgattc
     7021 tggaaagttt cgacatccac gtaccgaaag gttacatcta ctttgcgatg ttcttctcta
     7081 ttgcggttga aagcctcaac ctgattcgca acaaaaagaa tccgctctga tactccgtac
     7141 gctctcctgc gggagggcgt actcactcct gttaccctca cagtttaaga ttttcctgct
     7201 ttcaagatat ataacgtcgg tttataaaca gactattatc ataggtgggc caaatgagga
     7261 taaccggatg aaaaaatggg cagtaataat ttctgcagtc ggactggcgt ttgctgtttc
     7321 cgggtgttcc agtgattacg tcatggcgac caaagatggc cgtatgattt tgaccgatgg
     7381 aaaacctgaa attgatgatg ataccgggct ggtgagttat cacgatcagc aaggtaacgc
     7441 gatgcaaatt aaccgtgatg atgtttcgca aattattgaa cgttaacaaa taaggtcagc
     7501 atccggctgg ccttaagatt tttctctttc acttttccct tccctctgcc atttttatat
     7561 tccttatgtt gtgattataa aaaggaaacg gctatgcaat atcaccgtat accccacagt
     7621 tcgctggaag tcagcacgct ggggcttggc acgatgacgt ttggtgaaca gaacagcgaa
     7681 gccgacgccc acgcacaact cgactatgcc gtcgctcagg gcattaacct tatcgacgtt
     7741 gccgaaatgt acccagtacc tccgcgcccc gaaactcaag ggctaaccga aacctacgtc
     7801 ggcaactggc tggcgaaaca tggcagccgc gaaaagttaa ttatcgcctc caaagtgagc
     7861 ggaccgtcgc gcaataatga caaaggcatc cgcccggatc aggcgctgga tcggaagaat
     7921 atccgcgaag cgctgcatga cagcctcaag cgtctgcaga ctgattacct cgatctctat
     7981 caggtgcact ggccgcagcg cccaaccaac tgcttcggca aactcggtta tagctggaca
     8041 gattctgcgc ctgcggtttc gctgctggat acactggacg cactggcaga gtaccaacgc
     8101 gcgggaaaaa ttcgttatat cggcgtgtcg aacgaaactg catttggcgt aatgcgctac
     8161 ctgcatctgg cggacaaaca cgatctgccg cgtattgtca ccattcaaaa tccctacagt
     8221 ctgttaaacc gcagttttga agtaggtctg gcagaagtca gtcagtatga aggggtcgaa
     8281 ctgctggcct attcgtgcct gggtttcggc acgctgaccg ggaaatatct caatggtgca
     8341 aaacccgctg gcgcacgtaa tacgctcttt agtcggttca cccgctatag cggtgagcaa
     8401 acgcaaaaag ccgtcgcggc gtatgttgat atcgccagac gtcatggcct ggatcctgct
     8461 cagatggcgc tcgcttttgt acgccgtcaa ccgtttgttg ccagcactct gctgggcgca
     8521 accacgatgg agcagctgaa aactaacgtc gaaagtttgc atctggagtt aagcgaagac
     8581 gtattagctg aaattgaagc ggtgcatcag gtttatacgt atccggcacc gtgatccttt
     8641 cgcctggtgt tttgcgccag gcgtctcttt tctccacttt tcagaatgta ttcactactt
     8701 ttcgccgcat ctttcctgac aaagcgcaat agactctttt ggtcaacgcc agcgcgcgtt
     8761 tcaatgtctg tgcccattgt taaggataac catgttgatt ttcaaaccta ttcccctcgc
     8821 catgttgctt accgggtgcc tgtcctccgc ctggggggcg cagttaactc aagcgctaac
     8881 actcaaagac gtcactgttt atctgcatgg tgccagccta caaggtgaag agacactcaa
     8941 cttaccggca ggtgaaagcg aaattatttt cactaatatc gccaattcgg taaccgacga
     9001 cagcatcgcc atttctatcg ataacggagt gacggtgctt tcctcacgta tcgcgctaat
     9061 ggaaacacag ccgaatgcgc tgccgtcaga tactcagcaa cagttaaaag cagtaaaggt
     9121 ggaacaggca aaactgaccg ccgagaaaaa caatatcgcg gctcaaatta aggtattaga
     9181 ggcaaatagc acattaacca ataaaagtgc cgccgaagtt ggcaagtatc tgcaaatggt
     9241 gaaagagaaa aatggtgagt tattacgggc tgacaatgag ttgacgcaga aacttgctga
     9301 taaccagctt ggaattgaaa agttggagca acgcgagagt atgaataacg gtgattcaga
     9361 ggcatccgat gatccgcagg ttatcatcgc taaggtatat gtcccaaaag ccgtcactgc
     9421 gaaagcggtg gtgcattata taactcctga tgctacatgg acgccgatct atgatattca
     9481 cgccacgggc ataaaccaac cgctgcgtct ggtttacaag gctaatattc gccagtatac
     9541 ggggttgcca tggaaaaacg ttaatctcgt gctctcgacc agtgaacctg atactcatct
     9601 cacgctgcca cacctggaac ctctgtatgt ggatgtgact gagtatggtg gtgtaagcgg
     9661 ctcgctttca gcaacacccg tgatgaaaca gcgttcggaa tattcgtctg accaggatgc
     9721 atcagaattg cagaaatccc tggaggcaga gatgcaaaaa tcaagggagc gaccagccgg
     9781 gcgcaagtat gccgcacagg cagcaccagc ttcggcgcga ccagaaagtt tcaccgcgca
     9841 aaatgttcgc tatgcgcttt cgctgccgtg gcaaatcgac agcgacaaca ccgcgcgcac
     9901 ggtggtcatt aaggaggaat cggtcagcgg tgaatatcgc ttctttgcta ttccgaagat
     9961 cagtaatgac gcttatctcc aggtgcaaat taaagagtgg gaacagttaa atttaatccc
    10021 cggcgaaagt cagatcttct ttggtaacaa tcgtaccggc gtgggctatt tgcagccgcc
    10081 ggaagatggc aagtcgctgg atattcagct taaccgcgat cccaagctga ttattcaacg
    10141 taaagccgta ctcaaaaaag agaatgcgtt atctatcttt aatgacagcg cggtgcgcaa
    10201 cttcagttac acccttaatg taaagaacag ctacggcgac gcggtggata tcactctgct
    10261 cgaccagatc ccagtcagtg ctaatagtga tattacggtg gaaaaaatgc agtatggtca
    10321 ggcgcagttt gatgcgaaaa cgggtgaact gtcctggacg ctgaaactgt caggtaaaga
    10381 gaacgtgagt ctgccattct catacagcgt gaagtatccg aaaggtatgc acccttcggg
    10441 aatgtgacgt tcaccagatg gcggaatatg ccatctggtg aaagcttaat gacggcgctg
    10501 ccagatccac agcgccgtta ttgccagcgc aaacagcgca ccgaagccaa tgccaatggg
    10561 cacgaccggg atgcctacca ttaccgccag cgagtaaatg cccagcatca acaacatggc
    10621 gctgttttcg ccaaggtttt gtactgcaat cgcattcccc gccccgacgc tttttttacc
    10681 ccgctcctgt agcaacgcat tgagcggtac tacaaaaaag ccccccagca cgccaatcag
    10741 catcaacaag gcataggctg gcaacagctc gtgttgcagg gagaaaatca gtaccaccac
    10801 gccaatcaaa atcccggctg gcatacagcg tgacacggtt tccagcgtaa ccagcttcgc
    10861 tgccgcacct gcgccaacca cgataccaat cgctaccatc gcgttgagat aggtcggcgt
    10921 agcgttatcg gtaatgccca gcgccaccgg tacccacagc accaacagga aacgcagcgt
    10981 gacacctgct ccccagaata aactggtgcc caccagcgaa aaacgcgttt caccattgcg
    11041 ccatagcgag gtgcaggcat tcaggaaact gcgggtcatg ttgatgagat tccaggactg
    11101 ccccggacgc gccgccgcca gtttgggaat gtagatattg gcaacgaccg caccaccgta
    11161 ggccagtgcg catgcggcca gggcgaccag gacatgccag tcagccagca caccaccggc
    11221 tacggaaccg agcaaaatcg ccgctatggt agacgcttcc attaaaccgt tagcttttac
    11281 taacttacta cccgtggtta attcgccgag aataccgtat ttcgccggtg aataggctgc
    11341 agcaccaaca cccaccagcg tatagccgag aaacggattg ataccaaagc agatactggc
    11401 tgcgcccagc agcttcaggc cgttggcaaa catcatcacc cggcctttgg cgaagctatc
    11461 cgccacctgc ccgacaaacg gcgcaaaaag aatgtaagca cctacaaaca ccatttgcag
    11521 gatgggctgg ctccactccg gatagaactg cgcttttagt aacgccagag tggcaaacag
    11581 tagggcatta tcgccaaacg cagagagaaa ctgcgccacg ataaccgctt tcatcccctt
    11641 cgaccacaac gaagtgttag tgtgcactga ctcactcatc gtgttgttcc gcttcgtcta
    11701 cccagctttt caacgtgaca aagtcaggtt tgccgctgcc aagtaatggc atctgtttca
    11761 gatagcgaat atcgcgcggt acagcaagct ccggcacgcc gtgctcgcgg gcatactgtt
    11821 gtaacttatc gcgcgtcagt tcgttatctg tggtgaaaag caccagcgcc tcgcctttgc
    11881 tggcatcgct cttaatcgca gtggcatgga ctttatctgg cgaaacacca agtgccagtt
    11941 gttccaccat ttccagcgat accatttcgc ctgcaatttt ggcaaagcgt tttgcgcggc
    12001 cctgaatctg cacaaagccc tgctcgtcaa aacgcacgat atcgccagtg tcataccagc
    12061 cgcgctccat ttcgccgcga acattctcgg cggtgggcac ttccagtaca ccaggcttct
    12121 ccacccgcag atagccgttc attatgttcg gccctttcag ttgcaggcgt ccgccctctt
    12181 cgataccagg aaccgacaac aggcgcgcat ccattcctgg tagaatacgc cctaccgtac
    12241 cgggtttcgc cgccatcggt acgttgatgg aaacgacagg cgcgcactcg gtcacaccgt
    12301 agccttcaag gatgcgcagg ccaaatttat cctgccaaag ctgtttggta ctttcttgta
    12361 gtttttctgc gcctgccacc acatagcgta gacgatagaa gtcatacggg ttggcgaaac
    12421 gcgcgtagtg accgaggaaa gtcgaggtac cgaacaacac ggtgcaactg cggtcataca
    12481 ccaactccgg cacaatacgg taatgcagcg ggcttggata aaggaacact tctgcacctg
    12541 tgagcagtgg cgtaaacagg cctaccgtaa gcccaaagga gtgaaacagc ggtaacgccg
    12601 acataaagcg atcgttggtg gtgaagtcgg caatcgtttt aatctgctcg acgttcgcca
    12661 gaatgctttt atggctatgg acgacgcctt tcggatggcc ttcagaaccg gaggtaaaaa
    12721 ggatcagcgc ctcttcttcc ggctgctgtt taacctgtgc cagacgcggc atcagcaaat
    12781 gagcgaagat ccacactttg tcggcagtgg tgacatctgc ttttaaatct tccagataaa
    12841 cccagcgtac ctgagtaagc tgctccggca gatgccagag tttaccttta tcgagaaact
    12901 ggcgggaagt gaagatggtt ttgatttcag ccgccgtaat agcactggtc agccctttta
    12961 ccccggcggt gtagttcatc attgcgggaa tgcggcgacg ggcgatggcc ccaaaaatca
    13021 ctgccgcact gatgcctgca ttgggcagca ttaagccgat gcgttcgcct tcaacactgt
    13081 atttttcaag gatgcgtcca acaaacagcg ttttcgtaag caatttgcga taggagtctg
    13141 gggtaaagtt gacgtcttcg acacatttct tcccggctcc gaagcggtac attgcactca
    13201 gtaaagattc gtacagcgtt tcacgcgggc gcaccgccat acgcgcttcc atcattattt
    13261 gatgcagcat ttcgccagcg attttgcgac ggtcacgggc acgcggcgca tccggcatct
    13321 ccacctgcgt tggtggcaaa atatgcagcg taatttgcgg gaacaagcga cgtttaacca
    13381 gacctttcag gcggctgaag tgcgtaagtt ccgccccttc aatacgcaca ggaataaccg
    13441 ttgcaccaga cttcgccgcg acaaaacccg tgccatcgta gattttcatc agcgagcctg
    13501 tggtggtgat gcgtccttca gggaaaatca ccactggtcg gccctgttcc accagacgta
    13561 ccagatgttt aatcgccata ggctgcgtcg ggtcgagagg aacaaagtcg ataaatgatt
    13621 tcagccaacg catataccac tgttggctta ttgaggtgta aacggcaaat accggacgca
    13681 cgggtaaaaa caatgccagc agtatgccat caataaaaga gacgtgatta ggcgtgatta
    13741 gaacgcgctc gcccttcagt gcctggttgt cacccgtaac gcgaacgcga tacaaaacac
    13801 ggcacacatt tcgaaaaaag ctaaaaagca ttccaactcc ctttgctctg attcagtaaa
    13861 agcgaatgga gggagattac acgagataaa gaacgcgagc gacagtaaat taggtgcgaa
    13921 aaaaaacctg cgcatccgcg caggttggtg caagagacag ggtacgaaga gcgtaccgaa
    13981 taatctcacc aatcaatacc tctgggatct tgattgtggt ctgcacaacg actcttcgcc
    14041 agcgagaaaa cgcaaaggaa tgaagggaaa tgcaacgagg tgtgtaaatt gtcggttact
    14101 gttacagatt gatgaccggc aaaaaaaaac ctgcgcatct gcgcaggctg gtgtaattca
    14161 tgtgctcaac ccgaagttga cttcacctat caatacctct gggatcacca ctttagcaac
    14221 ctgaagccaa acgccaccag cggtcaatcg caacagcgtt tcgcaaagtg taaccaaagg
    14281 tttgcaatct ctttttttgt cttacttatt tgcgtttggc tcacattccc acgatgaaaa
    14341 cacgcccccc cttgaaccaa cgggcgtttt ccgtaacact gaaagaatgt aagcgtttac
    14401 ccactaaggt attttcatgg cgaccataaa ggatgtagcc cgactggcag gcgtttcagt
    14461 cgccaccgtt tcccgcgtca ttaataattc acccaaagcc agcgaagctt cccggcttgc
    14521 tgtgcatagt gcaatggagt ctcttagcta tcacccgaac gccaacgccc gtgcgctggc
    14581 gcagcagacc actgaaacgg tcggtctggt cgttggtgat gtttccgatc cgtttttcgg
    14641 tgcaatggtg aaagcggtcg aacaggtggc ttatcacact ggtaattttt tattgattgg
    14701 caacggttac cacaacgaac aaaaagagcg tcaggccatt gagcaactga tccgccatcg
    14761 ctgtgctgcg ctggttgtcc atgccaaaat gatcccggat gccgatttag cctcattaat
    14821 gaaacaaatg cccggtatgg tgctgatcaa ccgtatcctg cctggctttg aaaaccgttg
    14881 tattgctctg gacgatcgtt acggtgcctg gctggcaacg cgtcatttaa ttcagcaagg
    14941 tcatacccgc attggttatc tgtgctctaa ccactctatt tctgacgccg aagatcgtct
    15001 gcaagggtat tacgatgccc ttgctgaaag tggtattccg accaatgatc ggctggtgac
    15061 atttggcgaa ccagacgaaa gcggcggtga acaggcaatg accgagcttt tgggacgagg
    15121 aagaaatttc actgcggtag cctgttataa cgattcaatg gcggcgggcg cgatgggcgt
    15181 tctcaatgat aatggtattg atgtaccggg tgagatttcg ttaattggct ttgatgatgt
    15241 gctggtgtca cgctatgtgc gtccgcgtct gaccaccgtg cgttacccaa tcgtgacgat
    15301 ggcgacgcag gctgccgaac tggctttggc gctggcggat aatcgccctc tcccggaaat
    15361 cactaatgtc tttagtccga cgctggtacg tcgccattca gtgtcaactc cgtcgctgga
    15421 ggcaagtcat catgcaacca gcgactaacc gcagttaaag caattccagc gccagtaatt
    15481 cttcaatggt ctggcgacgg cgaatcaacc gcgcctgacc attatcaaac agaacttctg
    15541 gtaacagcgg acgactgttg tagttggatg acattgatgc gccatatgcc cccgtatcat
    15601 gcagtaccag ataatcaccc gccttcactt ccggtaagac gcgggtttca acatttcccc
    15661 cttcttgctg ggtaaagaca tcgcccgatt cacataacgg cccggcgacg acggtttcca
    15721 ccgttggcgc gtattccaga gaacgaccat cagctgtcag ggcactgata tggtggtaac
    15781 taccgtacat tgccgggcgc atcagatcgt tgaacccggc atcaaccagc acaaagtggc
    15841 ggctacccat ttgtttgacg ctccgcactt gagtaattaa tacgccagcc tgcgctacca
    15901 gaaagcgacc cggttcaatt tccagtttca caggatggcc caaatggcgg gcgatttgct
    15961 cacgcgcggc attccacaga ccataataat gttcggtatc aaccgcctct tcaccctgtt
    16021 gataaggaat agaaagccca ccgcccgcag aaatagcctg taaatcctga ccgaattcga
    16081 ggacctgacg caccatagca ccacatacct gttccagatg ggcataatca acgccagaac
    16141 caatgtgcat gtgaatgccg accagctgta gatgatgacg ttgtatcacg tccagtgcgg
    16201 cgggcagatc ggtgtaccag ataccgtgct tgctgttttc gccaccggta ttggtttttt
    16261 ggctatgccc gtgaccaaac cccggattaa cacgcagcca tacccgatgc cctggcgaaa
    16321 cctgacccag ttggtcgagc atatcaacag aacccgcatt caccggaatt tgcaattcac
    16381 tgacgcgttc aagcgtcgcc tgatcgataa catctgccgt aaaaacaata tcatcggggt
    16441 gcgtttgcgg attgtaaccc gccgccagtg cacgctctat ttcgcctaac gagacggaat
    16501 ccactttcac gccctgctca cgcattaagc gcaaaatatg aatattggaa caggctttct
    16561 gtgcaaagcg caccacatca aactgtttca gcgctgcaat ctgccgacga ataatttgcg
    16621 catcgtagac ccacaccggg cagccaaatt ctgcgggcaa acgcagcaga ttttcggcgg
    16681 tgagatcggt atcagtgctg aacagtgaat gtggcataac aaactccaga taagtgcttt
    16741 tttatgatta cgccacatca taaaaagaat aaaaaatatc gatttatgtc gagtctatgc
    16801 aaaaatgata tggattaccg gattgcgaga gagcgctaat ggccgccgtt aacttacgtc
    16861 atattgaaat ttttcatgcg gtaatgaccg ccggaagcct gactgaggcg gcacacctgc
    16921 tacacacctc acagccaacc gtcagccgcg aactggcgcg ctttgagaag gttatcgggc
    16981 tgaaattgtt tgagcgcata cgtggacgat tacatcctac cgtgcaagga ctgcgtctgt
    17041 ttgaagaagt gcaacgatcc tggtacggac tggatcgcat tgtcagtgcc gcagaaagtc
    17101 tgcgcgagtt tcgccaggga gaactgtcta ttgcctgcct gccggtcttt tcgcaatctt
    17161 ttttaccgca gctcctgcaa ccctttctgg cacgttatcc cgatgtcagc ttaaacatcg
    17221 tgccccagga atcaccgcta cttgaagagt ggctctcggc ccagcgtcat gatttaggac
    17281 tcactgagac actccatacg cctgcgggaa cagaacgtac cgaattactc tctttagatg
    17341 aagtgtgtgt gttacctccg ggccatccgc tggcggtaaa aaaggtatta acgccggatg
    17401 attttcacgg tgaaaactac atcagccttt cccgtactga cagctatcgc cagttcctgg
    17461 atcaattgtt tactgagaat caggttaaac gacgcatgat cgtagaaacc cacagcgccg
    17521 cgtcagtctg cgcaatggta cgggcggggg taggcgtttc ggtagttaac ccactcaccg
    17581 cactggatta tgcggcaagc ggtttagtgg tgcggcggtt cagcattgcg gttccgttca
    17641 ctgtcagcct gatccgcccc ctgcaccgcc cgtcatcagc gctggtgcag gcgtttagtg
    17701 agcatttaca agcgggatta ccgaaactgg tcacttctct tgacgctatt ttgtcgtcag
    17761 ctacgacagc ataaaagcga cagcatcctc ggcatggatc gccgcggtat caaacacagg
    17821 cagaacactg cgctcttctg gcaccagtaa accaatttct gtgcagccaa aaatgacgcc
    17881 ctgtgcg
//
LOCUS       DAMZFT010000098        17882 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.098, whole genome shotgun sequence.
ACCESSION   DAMZFT010000098 DAMZFT010000000
VERSION     DAMZFT010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17882)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17882)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17882
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.098"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(<1..155)
                     /locus_tag="PN977_003486"
     CDS             complement(<1..155)
                     /locus_tag="PN977_003486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HDI6043488.1"
                     /translation="MLEMLMQWYRRRFSDPEAIALLVILVAGFGIIFFFSGLLAPLLV
                     AIVLAYLL"
     gene            368..1831
                     /gene="bepA"
                     /locus_tag="PN977_003487"
     CDS             368..1831
                     /gene="bepA"
                     /locus_tag="PN977_003487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-barrel assembly-enhancing protease"
                     /protein_id="HDI6043489.1"
                     /translation="MFRQLKKNLVATLIAAMTIGQVAPAFADSADTLPDMGTSAGSTL
                     SIGQEMQMGDYYVRQLRGSAPLINDPLLTQYINSLGMRLVSHANSVKTPFHFFLINND
                     EINAFAFFGGNVVLHSALFRYSDNESQLASVMAHEISHVTQRHLARAMEDQQRSAPLT
                     WVGALGSILLAMASPQAGMAALTGTLAGTRQGMISFTQQNEQEADRIGIQVLQRSGFD
                     PQAMPTFLEKLLDQARYSSRPPEILLTHPLPESRLADARNRANQMRPMVVQSSEDFYL
                     AKVRTLGMYNSGRNQLTSDLLDEWAKGNVRQQRAAQYGRALQAMEANKYDEARKTLQP
                     LLAAEPGNAWYLDLATDIDLGQNKANEAINRLKNARDLRTNPVLQLNLANAYLQGGQP
                     QEAANILNRYTFNNKDDSNGWDLLAQAEAALNNRDQELAARAEGYALAGRLDQAISLL
                     SSASSQVKLGSLQQARYDARIDQLRQLQERFKPYTKM"
     gene            1852..2211
                     /gene="arsC"
                     /locus_tag="PN977_003488"
     CDS             1852..2211
                     /gene="arsC"
                     /locus_tag="PN977_003488"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311384.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HDI6043490.1"
                     /translation="MTKQVKIYHNPRCSKSRETLNLLKENGVEPEVVLYLQTPADAAT
                     LRDLLKMLGMNSARELMRQKEDLYKELNLADSSLSEGALIQAMVDNPKLMERPIVVAN
                     GKARIGRPPEQVLEIVG"
     gene            complement(2317..3063)
                     /gene="hda"
                     /locus_tag="PN977_003489"
     CDS             complement(2317..3063)
                     /gene="hda"
                     /locus_tag="PN977_003489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA inactivator Hda"
                     /protein_id="HDI6043491.1"
                     /translation="MVNFSRFCEILVEVSLNTPAQLSLPLYLPDDETFASFWPGDNSS
                     LLAALQNVLRQEHSGYIYLWAREGAGRSHLLHAACAELSQRGDAVGYVPLDKRTWFVP
                     EVLDGMEHLSLVCIDNIECIAGDELWEMAIFDLYNRILESGKTRLLITGDRPPRQLNL
                     GLPDLASRLDWGQIYKLQPLSDEDKLQALQLRARLRGFELPEDVGRFLLKRLDREMRT
                     LFMTLDQLDRASITAQRKLTIPFVKEILKL"
     gene            complement(3113..4402)
                     /gene="uraA"
                     /locus_tag="PN977_003490"
     CDS             complement(3113..4402)
                     /gene="uraA"
                     /locus_tag="PN977_003490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HDI6043492.1"
                     /translation="MTRRAIGVSERPPLLQTIPLSLQHLFAMFGATVLVPVLFHINPA
                     TVLLFNGIGTLLYLFICKGKIPAYLGSSFAFISPVLLLLPLGYEVALGGFIMCGVLFC
                     LVSFIVKKAGTGWLDVLFPPAAMGAIVAVIGLELAGVAAGMAGLLPAEGQTPDSKTII
                     ISITTLAVTVLGSVLFRGFLAIIPILIGVLVGYALSFAMGIVDTTPIINAHWFALPTL
                     YTPRFEWFAILTILPAALVVIAEHVGHLVVTANIVKKDLLRDPGLHRSMFANGLSTVI
                     SGFFGSTPNTTYGENIGVMAITRVYSTWVIGGAAIFAILLSCVGKLAAAIQMIPLPVM
                     GGVSLLLYGVIGASGIRVLIESKVDYNKAQNLILTSVILIIGVSGAKVNIGAAELKGM
                     ALATIVGIGLSLIFKLISMLRPEEVVLDAEDADITDK"
     gene            complement(4488..5114)
                     /gene="upp"
                     /locus_tag="PN977_003491"
     CDS             complement(4488..5114)
                     /gene="upp"
                     /locus_tag="PN977_003491"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HDI6043493.1"
                     /translation="MKIVEVKHPLVKHKLGLMREQDISTKRFRELASEVGSLLTYEAT
                     ADLETEKVTIEGWNGPVEIDQIKGKKITVVPILRAGLGMMDGVLENVPSARISVVGMY
                     RNEETLEPVPYFQKLVSNIDERMALIVDPMLATGGSVIATIDLLKKAGCSSIKVLVLV
                     AAPEGIAALEKAHPDVELYTASIDQGLNEHGYIIPGLGDAGDKIFGTK"
     gene            5439..6476
                     /gene="purM"
                     /locus_tag="PN977_003492"
     CDS             5439..6476
                     /gene="purM"
                     /locus_tag="PN977_003492"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HDI6043494.1"
                     /translation="MTDKTSLSYKDAGVDIDAGNALVGRIKGVVKKTRRPEVMGGLGG
                     FGALCALPQKYREPVLVSGTDGVGTKLRLAMDLKRHDTIGIDLVAMCVNDLVVQGAEP
                     LFFLDYYATGKLDVDTASAVISGIAEGCLQSGCSLVGGETAEMPGMYHGEDYDVAGFC
                     VGVVEKSEIIDGSKVSDGDVLIALGSSGPHSNGYSLVRKILEVSGCDPQTTELDGKPL
                     ADHLLAPTRIYVKSVLELIEKVDVHAIAHLTGGGFWENIPRVLPDNTQAVIDESSWQW
                     PEVFNWLQTAGNVERHEMYRTFNCGVGMIIALPAPEVDKALALLNANGENAWKIGIIK
                     ASDSEQRVVIE"
     gene            6476..7114
                     /gene="purN"
                     /locus_tag="PN977_003493"
     CDS             6476..7114
                     /gene="purN"
                     /locus_tag="PN977_003493"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HDI6043495.1"
                     /translation="MNIVVLISGNGSNLQAIIDACKTNKIKGTVRAVFSNKADAFGLE
                     RARQAGIATHTLITSAFDSREAYDRELIHEIDMYAPDVVVLAGFMRILSPAFVSHYAG
                     RLLNIHPSLLPKYPGLHTHRQALENGDEEHGTSVHFVTDELDGGPVILQAKVPVFAGD
                     TEDDITARVQTQEHAIYPLVISWFADGRLKMHENAAWLDGQRLPPQGYAADE"
     gene            7286..9352
                     /gene="ppk1"
                     /locus_tag="PN977_003494"
     CDS             7286..9352
                     /gene="ppk1"
                     /locus_tag="PN977_003494"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HDI6043496.1"
                     /translation="MGQEKLYIEKELSWLSFNERVLQEAADKSNPLIERMRFLGIYSN
                     NLDEFYKVRFAELKRRIIISEEQGSNSHSRHLLGKIQSRVLKADQEFDGLYNELLLEM
                     ARNQIFLINERQLSVNQQNWLRHYFKQYLRQHITPILINPDTDLVQFLKDDYTYLAVE
                     IIRGDTIRYALLEIPSDKVPRFVNLPPEAPRRRKPMILLDNILRYCLDDIFKGFFDYD
                     ALNAYSMKMTRDAEYDLVHEMEASLMELMSSSLKQRLTAEPVRFVYQRDMPNALVEVL
                     REKLTISRYDSIVPGGRYHNFKDFINFPNVGKANLVNKPLPRLRHIWFDKAQFRNGFD
                     AIRERDVLLYYPYHTFEHVLELLRQASFDPSVLAIKINIYRVAKDSRIIDSMIHAAHN
                     GKKVTVVVELQARFDEEANIHWAKRLTEAGVHVIFSAPGLKIHAKLFLISRKENGEVV
                     RYAHIGTGNFNEKTARLYTDYSLLTADARITNEVRRVFNFIENPYRPVTFDYLMVSPQ
                     NSRRLLYEMVDREIANAQQGLPSGITLKLNNLVDKGLVDRLYAASSSGVPVNLLVRGM
                     CSLIPNLEGISDNIRAISIVDRYLEHDRVYIFENGGDKKVYLSSADWMTRNIDYRIEV
                     ATPLLDPRLKQRVLDIIDILFSDTVKARYIDKELSNRYVPRGNRRKVRAQLAIYDYIK
                     SLEQPE"
     gene            9357..10898
                     /gene="ppx"
                     /locus_tag="PN977_003495"
     CDS             9357..10898
                     /gene="ppx"
                     /locus_tag="PN977_003495"
                     /EC_number="3.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HDI6043497.1"
                     /translation="MPIHDKSPRPQEFAAVDLGSNSFHMVIARVVDGAMQIIGRLKQR
                     VHLADGLGPDNMLSEEAMTRGLNCLSLFAERLQGFSPASVCIVGTHTLRQALNATDFL
                     KRAEKVIPYPIEIISGNEEARLIFMGVEHTQPEKGRKLVIDIGGGSTELVIGENFEPI
                     LVESRRMGCVSFAQLYFPGGVINKENFQRARMAAAQKLETLTWQFRIQGWNVAMGASG
                     TIKAAHEVLMEMGEKDGIITPERLEKLVKEVLRHRNFASLSLPGLSEERKTVFVPGLA
                     ILCGVFDALAIRELRLSDGALREGVLYEMEGRFRHQDVRSRTASSLANQYHIDSEQAR
                     RVLDTTMQMYEQWREQQPKLAHPQLEALLRWAAMLHEVGLNINHSGLHRHSAYILQNS
                     DLPGFNQEQQLMMATLVRYHRKAIKLDDLPRFTLFKKKQFLPLIQLLRLGVLLNNQRQ
                     ATTTPPTLTLITDDSHWTLRFPHDWFSQNALVLLDLEKEQEYWEGVAGWRLKIEEEST
                     PEIAA"
     gene            complement(10937..13180)
                     /gene="pdeF"
                     /locus_tag="PN977_003496"
     CDS             complement(10937..13180)
                     /gene="pdeF"
                     /locus_tag="PN977_003496"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeF"
                     /protein_id="HDI6043498.1"
                     /translation="MKLNATYIKIRDKWWGLPLFLPSLILPIFAHINTFAHISSGEVF
                     LFYLPLALMISMMMFFSWAALPGIALGIFVRKYAELGFYETLSLTANFIIIIILCWGG
                     YRVFTPRRNNVSHGDTRLISQRIFWQIVFPATLFLILFQFAAFVGLLASRENLVGVMP
                     FNLGTLINYQALLVGNLIGVPLCYFIIRVVRNPFYLRSYYSQLKQQVDVKVTKKEFAL
                     WLLALGALLLLLCMPLNEKSTIFSTNYTLSLLLPLMMWGAMRYGYKLISLLWAVVLMI
                     SIHSYQNYIPIYPGYTTQLTITSSSYLVFSFIVNYMAVLATRQRAVVRRIQRLAYVDP
                     VVHLPNVRALNRALRDAPWSALCYLRIPGMEMLVKNYGIMLRIQYKQKLSHWLSPLLE
                     PGEDVYQLSGNDLALRLNTESHQERITELDSHLKQFRFFWDGMPMQPQIGVSYCYVRS
                     PVNHIYLLLGELNTVAELSIVTNAPENMQRRGAMYLQRELKDKVAMMNRLQQALEHNH
                     FFLMAQPITGMRGDVYHEILLRMKGENDELISPDSFLPVAHEFGLSSSIDMWVIEHTL
                     QFMAENRAKMPAHRFAINLSPTSVCQARFPVEVSQLLAKYQIEAWQLIFEVTESNALT
                     NVKQAQITLQHLQELGCQIAIDDFGTGYASYARLKNVNADLLKIDGSFIRNIVSNSLD
                     YQIVASICHLARMKKMRVVAEYVENEEIREAVFSLGIDYMQGYLIGKPQPLIDTLNEI
                     EPIRESA"
     gene            complement(13362..13514)
                     /locus_tag="PN977_003497"
     CDS             complement(13362..13514)
                     /locus_tag="PN977_003497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6043499.1"
                     /translation="MSHAVDSTYIKAQQDKMVNSSLCKKASVDTYFNVMEVNLIIYND
                     DVIMMN"
     gene            13532..13723
                     /locus_tag="PN977_003498"
     CDS             13532..13723
                     /locus_tag="PN977_003498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2633 family protein"
                     /protein_id="HDI6043500.1"
                     /translation="MSQATSMRKRHRFNSRMTRIVLLISFIFFFGRFIYSSVGAWQHH
                     QSKKEAQQSTLSVESPVQR"
     gene            14034..14552
                     /locus_tag="PN977_003499"
     CDS             14034..14552
                     /locus_tag="PN977_003499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HDI6043501.1"
                     /translation="MMKFKKCLLPVAMLASFTLAGCQSNADDHAADVYQTDQLNTKQE
                     TKTVNIISILPAKVAVDNSQNKRNAQAFGALIGAVAGGVIGHNVGSGSNSGTTAGAVG
                     GGAVGAAAGSMVNDKTLVEGVSLTYKEGTKVYTSTQVGKECQFTTGLAVVITTTYNET
                     RIQPNTKCPEKS"
     gene            14568..15107
                     /locus_tag="PN977_003500"
     CDS             14568..15107
                     /locus_tag="PN977_003500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5384 family protein"
                     /protein_id="HDI6043502.1"
                     /translation="MKKVFLCAILASLSYPAIASSLQDQLSAVAEAEQQGKNEEQRQH
                     DEWVAERNREIQQEKQRRANAQAAANKRAATAAANKKARQDKLDAEATADKKRDQSYE
                     DELRSLEIQKQKLALAKEEARVKRENEFIDQELKHKAAQTDVVQSEADANRNMTEGGR
                     DLMKSVGKAEENKSDSWFN"
     gene            complement(15327..15809)
                     /locus_tag="PN977_003501"
     CDS             complement(15327..15809)
                     /locus_tag="PN977_003501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001375980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2514 domain-containing protein"
                     /protein_id="HDI6043503.1"
                     /translation="MIWAFVKAYRKQLIIMAVLAVLVISGVVAWSVHGSRQYDAGYAQ
                     AKEDRKTEDERIRQHYEQEKAINEREAQQRIDQARNDALDAAARAGRLQQQLVAIREQ
                     LRQYNAIVGAGTSAADTGVLLADVLSKSLERNRQLAEYADRAAEAGRVCEKQYDTLTR
                     "
     gene            complement(15806..16435)
                     /locus_tag="PN977_003502"
     CDS             complement(15806..16435)
                     /locus_tag="PN977_003502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000403801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 19 protein"
                     /protein_id="HDI6043504.1"
                     /translation="MEIKQFQRAAGISEALAARWFSHITSAMKEFGISKPEDQAMFIA
                     QVGHESGGFTRLQENFNYSVSGLANFVRAGRLTQGQANALGRRAGEPPLPLERQRAIA
                     NLVYSKRMGNNAPGDGWNYRGRGLIQVTGLNNYRDCGNGLKVDLLESPELLAQDEYAA
                     RSAAWFFASKGCMKYTGDIARVTLIINGGRNGIDDRRARYINASKVLAV"
     gene            complement(16425..16733)
                     /locus_tag="PN977_003503"
     CDS             complement(16425..16733)
                     /locus_tag="PN977_003503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000256098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HDI6043505.1"
                     /translation="MVTHEFFLLITNAVICTGIAIRVVTFRRNGSQHRRWGGWLAYFL
                     IVAAASIPVRVAYAIWLRTPMAVDLSEVIINAVMLAAVIKTRGNVVQIFKVSRSKHGD
                     "
     gene            complement(16720..17124)
                     /locus_tag="PN977_003504"
     CDS             complement(16720..17124)
                     /locus_tag="PN977_003504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001275998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6043506.1"
                     /translation="MSAQLTSETLNQWLSMSSLAAVIAGVPPEVALGALAGAVIFVTS
                     AVEYPIRRRVLLSMLSFLCGLLFYKPAASILIGIASLIPTITQDSFEKGIVFSAGAFV
                     SAIVAVRIGIWLYHRSDNPRELIPGRKDDGNA"
     gene            complement(17327..17611)
                     /locus_tag="PN977_003505"
     CDS             complement(17327..17611)
                     /locus_tag="PN977_003505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000355605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6043507.1"
                     /translation="MDVTPFLHSLCAFVAQVLVGIFTGNWAYGAIAGCTFFIAREHTQ
                     AEYRWIEKFGKGKRINMPWWGGFDPRVWDVGSLLDFAVPVVFCFCVYFSI"
BASE COUNT         4807 a         4482 c         4315 g         4278 t
ORIGIN      
        1 agcaaatagg ccagcacaat agccaccagc aacggagcaa gcaggccact aaagaaaaag
       61 ataatgccaa atccggcaac taaaataacc agcaaggcaa tcgcttccgg gtcgctaaaa
      121 cggcggcgat accattgcat caacatttcg agcataaaga ccttccctga acctcaagag
      181 cgggattgcg atccgcaatt gtatcgaaat gtcacaaaaa agacttcgct ttttatgacg
      241 gattcaggaa actgaaaagt catttgagtg ggctaatctt cgccgttaca ctcaaaggcg
      301 gcgcggtggg aacgatattt cacagtatcg gtcaaatgac tatcttcaga aatacaggat
      361 agaggttatg ttcaggcagt tgaaaaaaaa cctggttgca accctcattg ctgctatgac
      421 cattggtcag gtagccccgg cgtttgccga cagcgcagac accttgccgg atatgggaac
      481 ctccgcagga agcacgcttt ccattggcca ggaaatgcag atgggcgact attatgtccg
      541 ccagctacgc ggcagcgcgc cgttaattaa tgacccgctg ttaacgcaat atattaattc
      601 gctggggatg cgtctggttt cgcatgccaa ttcggttaag acaccgtttc atttctttct
      661 gatcaacaac gacgaaatta acgcctttgc tttctttggc ggcaacgtgg tgctgcactc
      721 tgccctgttc cgttattccg ataacgaaag tcaactggct tcagttatgg cgcacgaaat
      781 ctcccacgtc acccaacgtc acctggcgcg agcgatggaa gatcagcagc gcagcgcgcc
      841 gctgacctgg gtcggcgcgt taggttctat tttactggcg atggccagtc cgcaggcggg
      901 gatggcggcg ctgaccggta cactggcggg aacgcgccag gggatgatca gtttcaccca
      961 gcaaaatgaa caggaagcgg accgcattgg tattcaggtg ctgcaacgct cgggattcga
     1021 tccgcaggcg atgccgacct tcctcgaaaa attactcgat caggcgcgtt actcctcgcg
     1081 cccaccagaa attctgctca ctcacccact accggaaagc cgtctggctg atgcccgtaa
     1141 ccgtgccaat cagatgcgcc cgatggtggt gcaatcgtca gaagatttct atctggcaaa
     1201 agtgcgcaca ctggggatgt ataattccgg acgtaaccag ctcaccagtg atttgctgga
     1261 tgaatgggcg aaaggaaacg ttcgtcagca acgagcggcg caatatggtc gtgctctaca
     1321 ggcgatggaa gccaataaat acgatgaggc gcgtaaaacg ctgcaaccgt tactggcggc
     1381 agaacctggt aacgcatggt atctcgatct ggctaccgat atcgatcttg ggcaaaacaa
     1441 agccaatgag gcaatcaatc gcctgaaaaa tgcccgtgat ttgcgcacca atccggtgtt
     1501 gcagctcaac ctggcgaacg cttatctgca aggcggtcaa ccacaagaag cggccaatat
     1561 tctgaatcgc tacactttta ataataaaga tgacagcaac ggctgggatt tactggcaca
     1621 ggcggaagcc gcgctaaata accgcgatca ggaactggct gcgcgagcag aaggttatgc
     1681 gctcgccggg cgactcgatc aggccatttc cttgttgagt agcgccagtt cgcaggtgaa
     1741 attaggcagc ctgcaacaag cgcgttacga tgcgcgcatc gaccagttgc gccagctgca
     1801 ggaacgcttt aagccttata ccaagatgta attcgcatta aaggaagaac aatgaccaaa
     1861 caggtaaaaa tctaccataa cccacgctgt tcaaagagcc gggaaacgct gaacctgctg
     1921 aaagaaaacg gcgtggagcc agaagtggtg ctctaccttc agacacccgc cgatgcggca
     1981 acgctgcgtg atttgctgaa aatgctggga atgaatagcg ctcgtgaatt gatgcgccag
     2041 aaagaggatc tttataaaga actgaacctg gctgacagtt cgcttagcga aggggcgctg
     2101 attcaggcaa tggtggataa tccaaagcta atggagcgcc cgattgtggt cgcgaatggc
     2161 aaagcgcgga ttggtcgtcc accggagcag gtactggaaa tcgtgggtta agaagtgctc
     2221 catcgctagt tgaagcacat tgccggatgc gacgcttgcc gcgtcttatc cggccgacga
     2281 atactatggg tgcatttccg acaataaacg tcttgtctac aacttcagaa tttctttcac
     2341 aaacggaatg gtcagcttac gttgcgcggt aatcgacgca cgatccaact gatccagcgt
     2401 cataaataac gtgcgcattt ctctgtcgag ccgcttcagc aagaaacgcc ccacatcttc
     2461 cggcagttca aaaccacgca aacgagcgcg taactgtagc gcctgcaact tatcttcatc
     2521 agaaagtggc tgcaatttgt agatctgccc ccagtcgaga cgcgacgcga gatccggtaa
     2581 tcccagattc aactgccgcg gtggacgatc gccggtgatc aacaaccgtg ttttacccga
     2641 ttccagaatt cgattgtaga gatcgaaaat cgccatctcc cacaactcat cgcctgcaat
     2701 acactcaatg ttgtcgatac agaccagcga caaatgctcc ataccgtcga gcacttccgg
     2761 aacaaaccag gtgcgtttat ccagcgggac ataacccacc gcatcgccac gctgtgacaa
     2821 ttccgcgcaa gccgcgtgca gcagatggct gcgccccgcg ccttcgcgtg cccagagata
     2881 gatgtaaccg ctatgttcct gacgcagcac gttttgcagc gcggccagta aagaggagtt
     2941 atcccccggc cagaaacttg caaaggtttc gtcgtcagga agataaagtg gcaaagagag
     3001 ctgtgccggt gtgttcagag atacctcaac caggatttca caaaatcgcg agaagtttac
     3061 cacagaatcc catgatgttt gaaccgggca gcaacactgc ccggtcggta cattatttgt
     3121 ctgttatatc cgcgtcttct gcgtccagca ccacttcttc agggcgcagc atactaatca
     3181 gtttgaagat caaacttaag ccgataccga cgatggtcgc cagcgccata cctttcagct
     3241 ccgccgcgcc aatgtttacc ttcgcgccac tgacgccgat gatcaaaata acggaagtca
     3301 ggatcaggtt ctgtgctttg ttgtaatcca ctttcgattc gatcaacaca cgaataccgg
     3361 aagcaccgat gacaccgtaa agcagcagcg aaacgccgcc catcaccggc aatggaatca
     3421 tctggatagc agcagccagt ttaccgacgc aggaaagcag gatagcgaaa atcgccgcac
     3481 cgccgataac ccaggtactg taaacacggg tgatcgccat cacgccaatg ttttctccgt
     3541 aagtggtgtt tggcgtggag ccaaagaagc cggagatcac ggtcgacaag ccgttagcaa
     3601 acatcgaacg gtgcagacct ggatcgcgca gcagatcttt tttgacgata ttagccgtta
     3661 ccaccaggtg ccctacgtgt tcggcaataa ccactaacgc cgctggtaga atagtcagaa
     3721 tggcaaacca ctcgaagcgc ggcgtataga gggttggcag cgcaaaccag tgagcattaa
     3781 taatcggcgt ggtatcgaca attcccattg cgaaagagag cgcgtagccc accagcacgc
     3841 caattaaaat cgggataatt gccaggaaac cacgaaacag cacggaacct aaaaccgtga
     3901 ccgccagggt ggtaatggag atgatgatgg ttttggagtc tggcgtttgc ccttcagccg
     3961 ggagtaaacc tgccataccg gcagctacgc ccgccaactc cagaccgatg acggcaacga
     4021 ttgcgcccat tgccgcaggt ggaaacagca cgtccagcca gccggtcccc gctttcttca
     4081 cgataaaaga aaccaggcag aacagcacgc cgcacataat aaagccgccc agcgcgactt
     4141 cataccctaa cggcaacagt aacaataccg gtgaaataaa ggcaaagctg gaaccaagat
     4201 aagccgggat tttcccttta cagatgaaga gatacagcag cgttcctata ccgttaaata
     4261 acagtacagt cgccgggtta atatgaaata agacgggcac caggacggtt gcaccaaaca
     4321 tggcgaacaa atgttgcaaa ctaagcggga ttgtctgtaa aagtggcggt ctttcactca
     4381 ccccgatagc acggcgcgtc atagtattat cctctgtatt atgtgttata ggcgctttac
     4441 tcaaaaaaaa gccgactctt aaagtcggct ttaattattt ttattcttta tttcgtacca
     4501 aagattttgt caccggcatc gccgaggccc ggaataatgt atccgtgctc gttcagtccc
     4561 tggtcaatcg atgcagtata cagttcgacg tccgggtgcg ctttttccag cgcagcgata
     4621 ccttctggcg cagctaccag caccagaact ttgatgctgc tgcagcccgc ttttttcagc
     4681 aggtcgatgg tcgcgataac ggaaccaccg gttgccagca ttgggtcaac gatcagcgcc
     4741 atacgctcat cgatgttaga aaccagtttc tggaagtacg gtaccggctc cagcgtttct
     4801 tcattacggt acataccgac gacgctgatg cgcgcgctcg gaacgttttc cagcacaccg
     4861 tccatcatcc ccagaccggc acgcagaatt ggcacaacgg taattttctt acctttgatc
     4921 tggtcgattt ctaccgggcc gttccagcct tcgatagtga ctttttccgt ttcgaggtcg
     4981 gcggtcgctt cgtaagtcag caggctaccc acttcggaag cgagttcgcg aaagcgcttg
     5041 gtgctgatat cttgctcacg catcagtccc agcttgtgtt tgacgagtgg gtgtttgact
     5101 tccacgatct tcatactctt tctcctttga ggggcagcca caaaaaaaat cgacggatta
     5161 tacctccttt cttcaaggcg gcaatattat tttcgttgac tttagtcaaa atgataacgg
     5221 tttgagataa agttatttta tattcaaatg gttatggaag aagattattc catccgaaaa
     5281 ctaaccttta ccctggtaca agtattcttt tgccgcgcgc ctggggaaaa gacgtacaaa
     5341 aaggttgtgt aaagcagtct cgcaaacgtt tgctttccct gttagaattg cgccgaattt
     5401 tatttttcta ccgcaagtaa cgcgtgggga cccaagcagt gaccgataaa acctctctta
     5461 gctacaaaga tgccggtgtt gatattgacg cgggtaatgc tctggttgga agaatcaaag
     5521 gcgtagtgaa gaaaacgcgt cgtccggaag tgatgggcgg tctgggcggc ttcggtgcgc
     5581 tgtgtgcatt gccgcaaaaa tatcgtgaac ctgtgctggt ttccggcact gacggcgtag
     5641 gtaccaagct gcgtctggcg atggacttaa aacgtcacga caccattggt attgatctgg
     5701 tcgccatgtg cgttaatgac ctggtggtgc aaggtgcgga accgctgttt ttcctcgact
     5761 attacgcaac cggaaaactg gatgttgata ccgcttcagc ggtgatcagc ggcatcgcgg
     5821 aaggttgtct gcaatcgggc tgttcactgg tgggtggcga aacggcagaa atgccgggga
     5881 tgtatcacgg tgaagattac gatgtcgctg gtttctgcgt tggcgtggta gaaaaatcag
     5941 aaatcattga cggatctaaa gtcagcgacg gcgatgtgct gattgcactc ggctccagcg
     6001 gtccgcactc gaacggctat tcgctggtgc gcaaaattct tgaagtcagc ggttgtgatc
     6061 cgcaaaccac cgaacttgat ggtaagccat tagccgatca tctgctggca ccgacccgca
     6121 tttacgtgaa gtcagtgctg gagttgattg aaaaggtcga tgtgcatgcc attgcgcacc
     6181 tgaccggcgg cggcttctgg gaaaacattc cgcgcgtatt gccagataat acccaggcag
     6241 tgattgatga atcttcctgg cagtggccgg aagtgttcaa ctggctgcaa acggcaggta
     6301 acgttgagcg ccatgaaatg tatcgcacct tcaactgcgg cgtcggtatg attattgccc
     6361 ttcctgctcc ggaagtggat aaagccctcg ccctgctcaa tgccaacggt gaaaacgcgt
     6421 ggaaaatcgg tatcatcaaa gcctctgatt ccgaacaacg cgtggttatc gaataatgaa
     6481 tattgtggtg cttatttccg gcaacggaag taatttgcag gcaattattg acgcctgcaa
     6541 aaccaacaaa attaaaggca ccgtacgggc agttttcagc aataaggccg acgcgttcgg
     6601 ccttgaacgc gcccgccagg cgggtattgc aacacatacg ctcatcacca gcgcgtttga
     6661 cagtcgtgaa gcctatgacc gggagttgat tcatgaaatc gacatgtacg cacccgatgt
     6721 ggtcgtgctg gctggtttta tgcgcattct cagcccggcg tttgtctccc attatgccgg
     6781 gcgtttgctg aacattcacc cttctctgct gccgaaatat cccggattac acacccatcg
     6841 tcaggcgctg gaaaatggcg atgaggagca cggtacatcg gtgcatttcg tcaccgatga
     6901 actggacggt ggcccggtta ttttacaggc gaaagtcccg gtatttgctg gtgatacgga
     6961 agatgacatc accgcccgcg tgcaaaccca ggaacacgct atttatccac tggtgattag
     7021 ctggtttgcc gatggtcgtc tgaaaatgca cgaaaacgcc gcgtggctgg atggtcaacg
     7081 tctgccgccg cagggctacg ctgccgacga gtaatgcccc cgtagttaaa gcgccagctc
     7141 tgccgctggc gtttttcaat tcacctgtaa atcgcaagct ccagcagttt ttttccccct
     7201 tttctggcat agttggacat ctgccaatat tgctcgccat aatatccagg cagtgtcccg
     7261 tgaataaaac ggagtaaaag tggtaatggg tcaggaaaag ctatacatcg aaaaagagct
     7321 cagttggtta tcgttcaatg aacgcgtgct tcaggaagcg gcggacaaat ctaacccgct
     7381 gattgaaagg atgcgtttcc tggggatcta ttccaataac cttgatgagt tctataaagt
     7441 ccgcttcgct gaactgaagc gacgcatcat tattagcgaa gaacaaggct ccaactctca
     7501 ttcccgccat ttactgggca aaattcagtc ccgggtgctg aaagccgatc aggaattcga
     7561 cggcctctac aacgagttgt tgctggagat ggcgcgcaac cagatcttcc tgattaatga
     7621 acgccagctc tccgtcaatc aacaaaactg gctgcgtcat tattttaagc agtatctgcg
     7681 tcagcacatt acgccgattt taatcaatcc tgacactgat ttagtgcagt tcctgaaaga
     7741 tgattacacc tatctggcgg tggaaattat ccgtggcgat accatccgtt acgcgctgct
     7801 ggagatccca tcagataaag tgccgcgctt tgtgaattta ccgccagaag cgccgcgtcg
     7861 acgcaagccg atgattcttc tggataacat tctgcgttac tgccttgatg atattttcaa
     7921 aggcttcttt gattatgacg cgctgaatgc ctattcaatg aagatgaccc gcgatgccga
     7981 atacgattta gtgcatgaga tggaagccag cctgatggag ttgatgtctt ccagtctcaa
     8041 gcagcgttta actgctgagc cggtgcgttt tgtttatcag cgcgatatgc ccaatgcgct
     8101 ggttgaagtg ttacgcgaaa aactgactat ttcccgctac gactccatcg tccctggtgg
     8161 tcgttatcat aattttaaag actttattaa tttccccaat gtcggcaaag ccaatctggt
     8221 gaacaaacca ctgccgcgtt tacgccacat ttggtttgat aaagcccagt tccgcaatgg
     8281 ttttgatgct attcgcgaac gcgatgtgtt gctctattat ccttatcata cctttgagca
     8341 tgtactggaa ctgctgcgtc aggcttcgtt cgacccgagc gtactggcga ttaaaattaa
     8401 catttaccgc gtggcgaaag attcacgcat catcgactcg atgatccacg ccgcacacaa
     8461 cggtaagaaa gtcaccgtgg tggttgagtt acaggcgcgt ttcgacgaag aagccaacat
     8521 tcactgggcg aagcgcctga ctgaagcagg cgtgcacgtt atcttctctg cgccggggct
     8581 gaaaattcac gccaaactgt tcctgatttc acgtaaagaa aacggtgaag tggtgcgtta
     8641 cgcacacatc gggaccggga actttaacga aaaaaccgcg cgtctttata ctgactattc
     8701 gttgctgacc gccgatgcgc gcatcactaa cgaagtacgg cgggtgttca actttattga
     8761 aaacccatac cgtccggtga catttgatta tttaatggtg tcgccgcaaa actctcgccg
     8821 tctgttgtat gaaatggtgg accgcgagat cgccaacgcg cagcaagggc tgcccagtgg
     8881 tatcaccctg aagctaaata accttgtcga taaaggcctg gttgatcgtc tgtatgcggc
     8941 ctccagctcc ggcgtaccgg ttaatctgct ggtgcgcgga atgtgttctc tgatccccaa
     9001 tctggaaggc attagcgaca acattcgtgc catcagtatt gttgaccgtt accttgaaca
     9061 tgaccgggtt tatatttttg aaaatggcgg cgataaaaag gtctaccttt cttccgccga
     9121 ctggatgacg cgcaatattg attatcgtat tgaagtggcg acgccgctgc tcgatccgcg
     9181 cctgaagcag cgggtgctgg acatcatcga catattattc agcgatacag tcaaagcacg
     9241 ttatatcgat aaagaactca gtaatcgcta cgttccccgc ggcaatcgcc gcaaagtacg
     9301 ggcgcagttg gcgatttacg actacatcaa atcactcgaa caacctgaat aaccctatgc
     9361 caatacacga taaatcccct cgtccgcagg agtttgctgc ggtcgatctt ggttcaaaca
     9421 gttttcacat ggtcatagcc cgtgtggtag atggtgccat gcagattatt ggccgcctga
     9481 aacagcgggt gcatctggcg gacggcctgg ggccagataa tatgttgagt gaagaagcaa
     9541 tgacgcgcgg tttaaactgt ctgtcgctgt ttgccgaacg gctacaaggg ttttctcctg
     9601 ccagcgtctg tatagttggc acccatacac tgcgtcaggc gctgaacgcc actgactttc
     9661 tgaaacgcgc ggaaaaggtc attccctacc cgattgaaat tatttccggt aatgaagaag
     9721 cccgtctgat ttttatgggc gtggaacata cccaaccgga gaaaggtcgc aaactggtta
     9781 ttgatattgg cggcggatct acggaactgg tgattggtga aaatttcgaa cctattctcg
     9841 ttgaaagccg ccggatgggt tgtgtcagct ttgcccagct ttatttccct ggcggggtca
     9901 tcaataaaga gaatttccag cgcgctcgta tggcggcagc acaaaaactg gaaacgttaa
     9961 cctggcaatt ccgtattcag ggctggaacg tggcaatggg cgcttccggt accataaaag
    10021 ccgcccatga agtgttaatg gaaatgggcg agaaagacgg gataattacc ccggaacgtc
    10081 tggaaaaact ggtaaaagaa gttttacgtc accgtaattt cgcatcgctg agtttaccgg
    10141 gtctttccga agagcggaaa acagtcttcg ttcctggact ggcgatttta tgcggtgtgt
    10201 ttgatgcttt agccatccgt gaactgcgcc tttctgacgg ggcgttacgc gaaggcgtac
    10261 tgtatgaaat ggaaggacgt ttccgtcatc aggatgtgcg tagtcgcacc gccagcagcc
    10321 tcgccaacca gtatcacatc gacagcgaac aggcccgacg ggtgctggat accactatgc
    10381 aaatgtacga acagtggcgg gaacagcaac cgaagctggc gcatccgcaa ctggaggcgc
    10441 tactgcgatg ggccgccatg ctgcatgagg tcgggttgaa tatcaaccac agcggtttgc
    10501 atcgccactc cgcttatatt ctgcaaaaca gcgacttgcc gggttttaat caggaacagc
    10561 agttgatgat ggcgacgctg gtgcgctatc accgtaaagc gattaagctc gacgatctgc
    10621 cgcgctttac cttgtttaag aagaaacagt ttctgccact gattcagcta ttgcgccttg
    10681 gcgtattact caataatcaa cgtcaggcaa ccaccacacc gccaacattg acgctgatta
    10741 ccgatgacag tcactggaca ctgcgtttcc cgcatgactg gtttagtcag aatgcgctgg
    10801 tactgcttga tctggaaaag gagcaagaat actgggaagg cgtggctggc tggcggttga
    10861 aaattgaaga agaaagtacc ccagaaatcg ccgcttaatg ctttgcgggc cgacgagaaa
    10921 tgtcggcccg cattattcag gcactttcgc gaatgggttc gatttcattc agcgtatcaa
    10981 ttaacggttg cggcttacca ataagataac cctgcatata atcgatcccc aaagagaaca
    11041 ccgcctcgcg gatctcttcg ttttcaacgt actctgccac tacccgcatt ttcttcattc
    11101 gcgccaggtg gcaaatcgac gccactatct gataatccag actatttgac acaatattgc
    11161 ggataaaact gccgtcaatt ttaagcaaat cggcattcac atttttaagc cgcgcatagc
    11221 tggcatagcc ggtgccgaaa tcatcaatcg caatctggca gcctaattcc tgaagatgct
    11281 gcaaggtgat ttgcgcctgc ttaacattgg tcagagcatt actttcggtg acttcaaaaa
    11341 taagttgcca cgcttcaatt tgatatttag ccagcagctg actgacttca acgggaaaac
    11401 gcgcctgaca taccgaggtt ggcgacagat taatcgcaaa acggtgagcg ggcatcttcg
    11461 ctctgttttc agccataaat tgcagcgtat gctcaatgac ccacatgtcg atactcgacg
    11521 ataaaccaaa ttcgtgcgcg accggcaaga agctgtcggg gctgatcagt tcatcattct
    11581 cacctttcat gcgcagaaga atttcatggt agacatcacc acgcataccg gtaatcggct
    11641 gggccatcag gaaaaaatgg ttgtgttcca gcgcctgctg tagccgattc atcatcgcga
    11701 ctttatcttt caattcgcgt tgcaaataca ttgccccgcg acgctgcata ttttccgggg
    11761 cgttggtcac gatggaaagc tcggcgaccg tatttagctc tcccagcagc aggtagatat
    11821 gattcactgg cgagcgcaca tagcagtaac tgacgccaat ctgcggttgc atcggcattc
    11881 catcccaaaa gaaacgaaat tgcttgagat ggctatccag ttcggtaatg cgctcctggt
    11941 gcgattctgt attcagtcgc agcgcgagat cgttacccga aagctgataa acatcttcac
    12001 ccggttccag caagggtgac agccagtgag aaagtttttg cttgtattga atccgtagca
    12061 tgatgccata gttcttaacc agcatttcca tgccagggat gcgtaaataa caaagcgcag
    12121 accagggggc atcacgcaac gcgcgattca gggcgcgaac atttggcaga tgaaccaccg
    12181 ggtccacata cgcaagccgc tgaatgcgtc tgactaccgc tcgctgacgg gttgccagta
    12241 cagccatata attgacaata aaagagaata ccagataact ggaggaggtt atggtcagtt
    12301 gcgtggtata gccaggataa atgggaatgt aattttgata gctgtggatg ctgatcatca
    12361 acacgaccgc ccagagcagc gaaatcagct tataaccata gcgcatcgct ccccacatca
    12421 tcaggggcag caataatgac aaggtatagt tggtgctaaa aattgtgctt ttttcattta
    12481 acggcatgca taacagcagc agtaaagcac ctaatgccag tagccagagt gcgaactctt
    12541 ttttggtgac tttgacatca acctgctgtt ttaattgcga ataataactg cgtaaataaa
    12601 acggatttcg cactacacga atgatgaagt agcacagcgg gacaccaatc agattgccca
    12661 ccagtaaggc ctgataattg attaaggtcc cgaggttaaa aggcatgacg ccgaccagat
    12721 tttctctgct cgccagtaat cctacaaacg cagcaaactg gaaaagtatc agaaacagcg
    12781 ttgcaggaaa cacaatctgc cagaatatac gctgggaaat taaacgggta tcaccatgtg
    12841 aaacgttgtt acgccgggga gtaaagaccc tgtaaccgcc ccaacagaga atgataatga
    12901 taataaaatt agccgttaat gagagcgttt cgtaaaaacc cagctctgca tatttgcgga
    12961 caaaaatccc taaggcgatc cctggcaatg ccgcccagct gaaaaacatc atcatgctga
    13021 tcatcagagc cagaggcaga taaaagagaa aaacctcacc ggaagaaata tgcgcgaaag
    13081 tattaatgtg ggcgaaaatg ggcaagatta aagaaggcag gaacagcgga agcccccacc
    13141 atttatcacg tatttttata taagttgcat tcagtttcat agatgctcag cagaatcccc
    13201 cacatcctga aggaggtgta ttcagacagg catcccacct gacttcgaat gatgattatt
    13261 catcactata gagagcattg attctaagtg tcatatgaaa gttccaattg atatatatca
    13321 aacaaaataa ccctgattaa tgaattatta cgtttatcat gttaattcat cattattaca
    13381 tcatcattgt aaataattaa attaacttcc ataacattaa aatatgtatc cactgacgct
    13441 tttttacata acgaagaatt gaccattttg tcctgttgtg ccttaatgta agtactgtcc
    13501 acagcgtggg acatacttca aggaaccttt tgtgagtcag gcaaccagta tgcgaaaacg
    13561 acaccgattt aacagtcgca tgacccgtat cgtactgctc atcagcttta tcttcttctt
    13621 tggccgtttt atctactcgt ccgtcggtgc atggcagcac catcagagca aaaaagaagc
    13681 tcagcaatcc acactctccg tcgaatcacc ggtacaacgt tagcggttac cttctccact
    13741 ttcacagaac ataacggcac ctcgctgtcg gatgcttttg ctctttggga ttatcaaagc
    13801 ggcagatatt ctttcatctt aaattttacg tctttgtcct gactgatgtt tatcctgttt
    13861 ggctgcgaaa taaatataaa attaatacat atattttaat gatgtatttt tataaattat
    13921 ttcccacatg aattttaata aatttaatct attcctttat acgcaataca tttactttcc
    13981 tcttttgatg atcttaaatg tcttattttt cgtaatgtgt ataacaagga atagtgatga
    14041 aatttaaaaa atgtcttctg cctgtggcaa tgttagcgtc attcactctg gcaggatgcc
    14101 agtcaaatgc tgatgatcat gccgccgatg tttatcaaac cgatcaactg aataccaaac
    14161 aagaaactaa aaccgttaat attatttcca ttcttcccgc aaaagttgcc gtagacaact
    14221 cccaaaataa acgaaacgca caagccttcg gcgcacttat tggtgcagtc gctggcggtg
    14281 taatcggcca caacgtgggg tctggcagca attccggaac gacggcaggt gcagttggcg
    14341 gcggagctgt tggcgcggca gcgggttcta tggtgaatga taaaacctta gtggaaggtg
    14401 tttctctaac gtataaggaa ggcaccaaag tgtatacctc cacccaggtg ggtaaagagt
    14461 gccagtttac gacaggttta gccgttgtta ttaccacgac gtataacgaa acgcgtattc
    14521 agccaaatac caaatgtcct gaaaagagct aataatcagg aggagtcatg aagaaagttt
    14581 ttctttgcgc catcttagcc tccttaagct atccggctat cgcctcatca ttgcaggatc
    14641 aactctctgc tgtcgcagaa gcggaacagc aaggtaaaaa tgaagagcaa aggcagcatg
    14701 acgaatgggt cgcggagcgc aacagggaaa tccagcaaga gaagcaacgt cgcgcaaacg
    14761 cccaggccgc ggctaataaa agagcggcaa cggcagcagc gaataagaaa gctcgtcagg
    14821 ataaactgga cgccgaagcc actgcggaca aaaaacgcga tcaaagttat gaagatgagc
    14881 tacgcagctt agagattcag aaacaaaaac tggcgctggc gaaagaagaa gcccgcgtta
    14941 agcgagaaaa cgaatttatc gatcaggaac tgaagcacaa agctgcgcaa accgatgtgg
    15001 tgcaatctga agctgacgca aacagaaata tgactgaagg cggtcgcgat ctgatgaaaa
    15061 gcgtgggcaa agcagaagag aacaaatcgg acagctggtt taactaagcg atgtcagtaa
    15121 cttcaagcct atgattcgtg agtataaaaa accctctgta gtaacagagg gttttgttca
    15181 ttcatagtgc agggatcaaa atcattccca ctcaattatt tacgataacc ataaccaatt
    15241 gagtgataac atttttccaa atctcaattt ttatcgtacc gttttatata ccgtcaccgg
    15301 aaatcagtac catgaaaaat gccatgctat ctggtcaggg tgtcgtactg tttttcacag
    15361 actcttccgg cttcggctgc ccggtcagca tactctgcca gttgtctgtt tctctcgaga
    15421 gatttactga gcacgtcggc aagcaaaact ccggtgtctg cggctgacgt cccagcgccg
    15481 acaatggcgt tatactgcct gagctgctca cggatggcaa cgagctgttg ctgcaaccgg
    15541 ccagcgcgag cggcagcatc aagagcatca ttgcgcgcct ggtcgatcct ctgctgcgct
    15601 tcacgttcat tgatcgcttt ctcctgttcg tagtgctgac gaattctctc atcttcggtt
    15661 ttgcggtctt ctttcgcctg cgcatacccg gcgtcgtact gacgactgcc gtgtacactc
    15721 caggcaacaa ctcctgatat gaccagaaca gcaagcaccg ccatgataat caactgtttc
    15781 cggtatgctt ttacgaatgc ccagatcata ccgccagcac cttactggca ttgatgtatc
    15841 gcgcgcgccg gtcgtcgatg ccgttccggc caccattgat aatcagagtt acacgtgcaa
    15901 tatcgccggt atacttcatg catcctttgc tggcaaagaa ccacgccgcg ctacgagccg
    15961 cgtattcgtc ctgcgccagc agttcaggac tctccagcag gtcaacctta agaccgtttc
    16021 cacagtcacg atagttattc aaaccggtaa cctggataag cccgcgccca cggtagttcc
    16081 agccatcacc aggggcattg ttccccatgc gtttgctgta caccagattg gcaatcgctc
    16141 tctggcgctc aagtggcaat ggtggttcgc ctgcacgtcg ccccaatgcg ttggcctgtc
    16201 cctgagtgag acgcccagcc cgaacaaagt tagccagtcc gctgacgctg tagttgaaat
    16261 tctcctgcaa ccgggtgaag cctccagact catgcccgac ctgagcaata aacatagcct
    16321 gatcttctgg tttgctgata ccaaactctt tcatcgcaga agttatatgc gagaaccagc
    16381 gtgcggccag tgcctcgctg ataccagcag ctcgctggaa ttgtttaatc tccatgttta
    16441 gacctcgata ctttgaaaat ttgaacgacg ttaccgcgcg ttttaataac cgcagctagc
    16501 atgacagcgt tgataatgac ctcagataaa tccacagcca ttggcgtacg taaccagatt
    16561 gcataggcga cacgaacagg aatactggcc gcagcaacaa tcaggaaata agcaagccat
    16621 cctccccacc ttcggtgttg agagccgtta cgccggaatg tgacaacgcg aattgctatg
    16681 ccagtgcaaa taactgcatt ggtgataagc aaaaaaaact catgcgttac catcgtcttt
    16741 tctccccgga attaactcgc gtggattatc ggaacggtga tagagccata taccaatacg
    16801 cacagcgaca attgctgaca cgaatgcgcc tgcagagaaa acaatccctt tttcaaaaga
    16861 gtcctgcgtg atggtaggga tcaggctggc tatgccgata agaattgatg ctgctggttt
    16921 gtaaaagaga aggccgcaaa gaaagctgag catcgacagg agcacccggc gacggatggg
    16981 gtactctact gcagaggtaa caaaaattac cgctccagcc aaagcaccca aagcaacctc
    17041 aggaggaact cctgctatca ccgccgccag agaactcatg ctaagccact gatttaaagt
    17101 ttcactggtt agttgagctg acatgttttc caccgtttat atgcataaca acctcctgaa
    17161 cagtaaagca ttacgcataa taaaccattt atggtttttt gttactttaa catcgtcaat
    17221 tccctgtttt tcatgccgag aataaagctc accttttaca tgactctgaa gaaacaaaca
    17281 gtgaagagca ggttgaatac cgcaccattt aatggcgcgg tagcagtcaa atagagaaat
    17341 aaacacaaaa acaaaaaaca acaggaacag caaaatccaa cagacttccc acatcccaca
    17401 cgcgtggatc aaaacctccc caccacggca tgttgatacg tttccctttc ccaaactttt
    17461 ctatccagcg atattctgcc tgggtgtgtt cacgcgcaat gaagaacgta caaccggcta
    17521 tcgcaccgta agcccagttt ccggtaaaaa taccaaccag tacctgagcc acaaaagcgc
    17581 aaagcgagtg aagaaatgga gtgacatcaa tgctcttaat catgaaactt ccccattggt
    17641 tgaaaatatg gctcctccaa cgccaagtgt atttcgtgtt gtgctcaacc tcagagggct
    17701 ggagtcagaa ttagaatatc ctgtagcaga aataaaagat cctccactaa tagtcaactg
    17761 cggaacatcg gctatatctg aataagttct gtcagcaaca gtattattta tagccacata
    17821 tgatccaaaa aaacttctta ttgtttggtt tttaacttta cttccttcaa tggttatcct
    17881 ac
//
LOCUS       DAMZFT010000099        17827 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.099, whole genome shotgun sequence.
ACCESSION   DAMZFT010000099 DAMZFT010000000
VERSION     DAMZFT010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17827)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17827)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17827
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.099"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..821
                     /locus_tag="PN977_003506"
     CDS             <1..821
                     /locus_tag="PN977_003506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012311799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HDI6043508.1"
                     /translation="NDSYTRKKNYAWMTSNTSIDNEGHTTQNLGLTETLLDDGNLSYS
                     VQQGYNSEGKTANGSASMDYKGAFADARVGYNYSDNGSQQQLNYALSGSLVAHSQGIT
                     LGQSLGETNVLIAAPGAENTRVANSTGLKTDWRGYTVVPYATSYRENRIALDAASLKR
                     NVDLENAVVNVVPTKGALVLAEFNAHAGARVLMKTSKQGMPLRFGAMATLDGAQTISG
                     IIDDDGSLYMSGLPAKGTITVRWGDAPDQICHISYELTEQQINAAITRMDSVCE"
     gene            812..1882
                     /locus_tag="PN977_003507"
     CDS             812..1882
                     /locus_tag="PN977_003507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HDI6043509.1"
                     /translation="MRIISGDSSVLRLRLLLTAVLMLWGFHAAAYTGQCHTTQGNPYI
                     GVNFGVKTLEEEENTAGKVKDEFYQWSESNDYYVSCDCDKDNVRSGLWAFAADSPLVY
                     LGDNWYKINDYLAAKVLLGVKGNTPSAVPFENLGTGSDTRWHICDPGGQRLGGKGASG
                     NSGSFSLKILQPFVGSVVIPPMALARLYECYNIPASDSCTTTGTPVLVYYLSGTINSL
                     GSCSVNSGETIEVDLGDVFAANFRVVGHKPLGSRTAELSIPVRCNTGNAELVNVNLSL
                     TATSDPDYPQAIKTSRPGVGVVVTDSQNNIISPAGGTLPLSIPDDADSFARMNVYPVS
                     TTGVPPETGSFEATATVRINFD"
     gene            1894..2436
                     /locus_tag="PN977_003508"
     CDS             1894..2436
                     /locus_tag="PN977_003508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HDI6043510.1"
                     /translation="MKKNTIFQVVILSFTLLYMHVGIAGPEQVSMHIYGNVVDQGCDV
                     ATKSALQNIHIGDFNISDFQTANTVSTAADLNIDITGCAAGITGADVLFSGEADTLVP
                     TLLKLTDTGGSGGMATGIAVQILDAQSQQEIPLNQVLPLTPLKAGDNTLKYQLRYKST
                     KAGATGGNATAVLYFDLVYQ"
     gene            2444..2959
                     /locus_tag="PN977_003509"
     CDS             2444..2959
                     /locus_tag="PN977_003509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415463.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HDI6043511.1"
                     /translation="MLKQIIWILFLLGLTGGCELFAHDGTVNISGSFRRNTCVLAQDS
                     KQINVQLGDVSLTRFSHGNYGPEKSFIINLQDCGTDVSTVDVTFSGTPDGVQSEMLSI
                     ESGTDAASGLAIAILDDAKILIPLNQASKDYSLHSGKVPLTFYAQLRPVNSDVQSGKV
                     NASATFVLHYD"
     gene            2925..3662
                     /locus_tag="PN977_003510"
     CDS             2925..3662
                     /locus_tag="PN977_003510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
                     /protein_id="HDI6043512.1"
                     /translation="MPARHLYFIMTNTWNRLALLIFAVLSLLVASELQAGVVVGGTRF
                     IFPADRDSISILLTNTSQESWLINSKINRPTRWAGGEASTVPAPLLAAPPLILLKPGT
                     TGTLRLLRTESDILPVDRETLFELSIASVPSGKVENQSVKVAMRSVFKLFWRPEGLPG
                     DPLEAYQQLRWTRNSQGVQLTNPTPYYINLIQVSVNGKALSNAGVVPPKSQRQTSWCQ
                     AITPCHVSWRTINDYGGLSAKKEQNLP"
     gene            3773..4783
                     /gene="pyrD"
                     /locus_tag="PN977_003511"
     CDS             3773..4783
                     /gene="pyrD"
                     /locus_tag="PN977_003511"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HDI6043513.1"
                     /translation="MYYPFVRKALFQLDPERAHEFTFQQLRRITGTPFEALVRQKVPA
                     KPVNCMGLTFKNPLGLAAGLDKDGECIDALGAMGFGSIEIGTVTPRPQPGNDKPRLFR
                     LVDAEGLINRMGFNNLGVDNLVENVKKAHYDGVLGINIGKNKDTPVEQGKDDYLICME
                     KIYAYAGYIAINISSPNTPGLRTLQYGEALDDLLTAIKNKQNDLQAMHHKYVPIAVKI
                     APDLSEEELIQVADSLVRHNIDGVIATNTTLDRSLVQGMKNCDQTGGLSGRPLQLKST
                     EIIRRLSQELNGRLPIIGVGGIDSVIAAREKIAAGASLVQIYSGFIFKGPPLIKEIVT
                     HI"
     gene            4957..5499
                     /gene="zapC"
                     /locus_tag="PN977_003512"
     CDS             4957..5499
                     /gene="zapC"
                     /locus_tag="PN977_003512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415466.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapC"
                     /protein_id="HDI6043514.1"
                     /translation="MRIKPDDNWRWYYDEEHDRMMLDLANGMLFRSRFARKMLTPDAF
                     SPAGFCVDDAALYFSFEEKCRDFNLSKEQKAELVLNALVAIRYLKPQMPKSWHFVSHG
                     EMWVPMPGDAACVWLSDTHEQVNLLVVESGENAALCLLAQPCVVIAGRAMQLGDAIKI
                     MNDRLKPQVNVDSFSLEQAV"
     gene            complement(5496..6605)
                     /gene="ycbX"
                     /locus_tag="PN977_003513"
     CDS             complement(5496..6605)
                     /gene="ycbX"
                     /locus_tag="PN977_003513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-N-hydroxylaminopurine resistance protein YcbX"
                     /protein_id="HDI6043515.1"
                     /translation="MATLTRLFIHPVKSMRGIGLTHTLADVSGLAFDRIFMITEPDGT
                     FITARQFPLMVRFTPSPVHDGLHLTAPDGSSAYVRFADFATQDAPTEVWGTHFTARIA
                     PDAINKWLSGFFSREVQLRWVGPQMTRRVKRHNTVPLSFADGYPYLLANEASLRDLQQ
                     RCPASVKMEQFRPNLVVSGASAWEEDSWKVIRIGDVVFDVVKPCSRCIFTTVSPEKGQ
                     KHPAGEPLKTLQSFRTAQDNGDVDFGQNLIARNSGVIRVGDEVEILATAPAKIYGAGA
                     ADDTANITQQPDANVDIDWQGQAFRGNNQQVLLEQLENQGIRIPYSCRAGICGSCRVQ
                     LLEGEVTPLKKSAMGDDGTILCCSCVPKTALKLTR"
     gene            6703..8811
                     /gene="rlmKL"
                     /locus_tag="PN977_003514"
     CDS             6703..8811
                     /gene="rlmKL"
                     /locus_tag="PN977_003514"
                     /EC_number="2.1.1.173"
                     /EC_number="2.1.1.264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 23S rRNA
                     (guanine(2069)-N(7))-methyltransferase RlmK/23S rRNA
                     (guanine(2445)-N(2))-methyltransferase RlmL"
                     /protein_id="HDI6043516.1"
                     /translation="MNSLFASTARGLEELLKTELENLGAVECQVVQGGVHFKGDTRLV
                     YQSLMWSRLASRIMLPLGECKVYSDLDLYLGVQAINWTEMFNPGATFAVHFSGLNDTI
                     RNSQYGAMKVKDAIVDAFTRKNLPRPNVDRDAPDIRVNVWLHKETASIALDLSGDGLH
                     LRGYRDRAGIAPIKETLAAAIVMRSGWQPGTPLLDPMCGSGTLLIEAAMLATDRAPGL
                     HRGRWGFSGWAQHDEAIWQEVKAEAQTRARKGLAEYSSHFYGSDSDARVIQRARTNAR
                     LAGIGELITFEVKDVAQLANPLPKGPYGTVLSNPPYGERLDSEPALIALHSLLGRIMK
                     NQFGGWNLSLFSASPDLLSCLQLRADKQYKAKNGPLDCVQKNYHVAESTPDSKPAMAA
                     EDYANRLRKNLKKFEKWARQEGIECYRLYDADLPEYNVAVDRYADWVVVQEYAPPKTI
                     DAHKARQRLFDIIAATISVLGIAPNKLVLKTRERQKGKNQYQKLGEKGEFLEVTEYNA
                     HLWVNLTDYLDTGLFLDHRIARRMLGQMSKGKDFLNLFSYTGSATVHAGLGGARSTTT
                     VDMSRTYLEWAERNLRLNGLTGRAHRLIQADCLAWLREANEQFDLIFIDPPTFSNSKR
                     MEDAFDVQRDHLVLMKDLKRLLRAGGTIMFSNNKRGFRMDLDGLAKLGLKAQEITQKT
                     LSQDFARNRQIHNCWLITAA"
     gene            8823..10730
                     /locus_tag="PN977_003515"
     CDS             8823..10730
                     /locus_tag="PN977_003515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDI6043517.1"
                     /translation="MSLISMHGAWLSFSDAPLLDNAELHIEDNERVCLVGRNGAGKST
                     LMKILNREQGLDDGRIIYEQDLIVARLQQDPPRNVEGSVYDFVAEGIEEQAEYLKRYH
                     DLSRLVMNDPSEKNLNELAKVQEQLDHHNLWQLENRINEVLAQLGLDPNVALSSLSGG
                     WLRKAALGRALVSNPRVLLLDEPTNHLDIETIDWLEGFLKTFNGTIIFISHDRSFIRN
                     MATRIVDLDRGKLVTYPGNYDQYLLEKEEALRVEELQNAEFDRKLAQEEVWIRQGIKA
                     RRTRNEGRVRALKAMRRERSQRREVMGTAKMQVEEASRSGKIVFEMEDVCYQVDGKQL
                     VKDFSAQVLRGDKIALIGPNGCGKTTLLKLMLGQLQADSGRIHVGTKLEVAYFDQHRA
                     ELDPDKTVMDNLAEGKQEVMVNGKPRHVLGYLQDFLFHPKRAMTPVRALSGGERNRLL
                     LARLFLKPSNLLILDEPTNDLDVETLELLEELIDSYQGTVLLVSHDRQFVDNTVTECW
                     IFEGGGKIGRYVGGYHDARGQQEQYVALKQPAVKKTEEAAAAKAETVKRSSSKLSYKL
                     QRELEQLPQLLEDLEAKLEALQTQVADASFFSQPHEQTQKVLADMAAAEQELEQAFER
                     WEYLEALKNGG"
     gene            10860..12113
                     /gene="pqiA"
                     /locus_tag="PN977_003516"
     CDS             10860..12113
                     /gene="pqiA"
                     /locus_tag="PN977_003516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiA"
                     /protein_id="HDI6043518.1"
                     /translation="MCEHHHAAKHILCSQCDMLVALPRLEHGQKAACPRCGTTLTVAW
                     DAPRQRPTAYALAALFMLLLSNLFPFVNMNVAGVTSEITLLEIPGVLFSEDYASLGTF
                     FLLFVQLVPAFCLITILLLVNRAELPVRLKEQLARVLFQLKTWGMAEIFLAGVLVSFV
                     KLMAYGSIGVGSSFLPWCLFCVLQLRAFQCVDRRWLWDDIAPMPELRQPLKPGVTGIR
                     QGLRSCSCCTAILPADEPVCPRCGTKGYVRRRNSLQWTLALLVTSIMLYLPANILPIM
                     VTDLLGSKMPSTILAGVILLWSEGSYPVAAVIFLASIMVPTLKMIAIAWLCWDAKGHG
                     KRDSERMHLIYEVVEFVGRWSMIDVFVIAVLSALVRMGGLMSIYPAMGALMFALVVIM
                     TMFSAMTFDPRLSWDRQPESEHEES"
     gene            12118..13758
                     /gene="pqiB"
                     /locus_tag="PN977_003517"
     CDS             12118..13758
                     /gene="pqiB"
                     /locus_tag="PN977_003517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intermembrane transport protein PqiB"
                     /protein_id="HDI6043519.1"
                     /translation="MESNNGEAKIQKVKNWSPVWIFPIVTALIGAWVLFYHYSHQGPE
                     VTLITANAEGIEGGKTTIKSRSVDVGVVESATLADDLTHVEIKARLNSGMEKLLHKDT
                     VFWVVKPQIGREGISGLGTLLSGVYIELQPGAKGSKMDKYDLLDSPPLAPPDAKGIRV
                     ILDSKKAGQLSPGDPVLFRGYRVGSVETSTFDTQKRNISYQLFINAPYDRLVTSNVRF
                     WKDSGIAVDLTSAGMRVEMGSLTTLLSGGVSFDVPEGLDLGQPVAPKTAFVLYDDQKS
                     IQDSLYTDHIDYLMFFKDSVRGLQPGAPVEFRGIRLGTVSKVPFFAPNMRQTFNDDYR
                     IPVLIRIEPERLKMQLGENADVVEHLGELLKRGLRGSLKTGNLVTGALYVDLDFYPNT
                     PAITGIREFNGYQIIPTVSGGLAQIQQRLMEALDKINKLPLNPMIEQATSTLSESQRT
                     MKNLQTTLDSMNKILASQSMQQLPTDMQSTLRELNRSMQGFQPGSAAYNKMVADMQRL
                     DQVLRELQPVLKTLNEKSNALVFEAKDKKDPEPKRAKQ"
     gene            13755..14318
                     /gene="pqiC"
                     /locus_tag="PN977_003518"
     CDS             13755..14318
                     /gene="pqiC"
                     /locus_tag="PN977_003518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309063.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiC"
                     /protein_id="HDI6043520.1"
                     /translation="MKKWLVTIAALWLAGCSSGEINKNYYQLPVVQGGTQSTASQGNR
                     LLWVEQVAVPDYLAGNGVVYQTSDVKYVIANNNLWASPLDQQLRNTLVANLSTQLPGW
                     VVASQPLGSAQDTLNVTVTEFNGRYDGKVIVSGEWLLNHQGQLIKRPFRLEGVQTQDG
                     YDEMVKVLASVWSQEAASIAQEIKRLP"
     gene            14574..14741
                     /gene="rmf"
                     /locus_tag="PN977_003519"
     CDS             14574..14741
                     /gene="rmf"
                     /locus_tag="PN977_003519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome modulation factor"
                     /protein_id="HDI6043521.1"
                     /translation="MKRQKRDRLERAHQRGYQAGIAGRSKEMCPYQTLNQRSQWLGGW
                     REAMADRVVMA"
     gene            complement(14811..15329)
                     /gene="fabA"
                     /locus_tag="PN977_003520"
     CDS             complement(14811..15329)
                     /gene="fabA"
                     /locus_tag="PN977_003520"
                     /EC_number="4.2.1.59"
                     /EC_number="5.3.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014915875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-hydroxydecanoyl-ACP
                     dehydratase/trans-2-decenoyl-ACP isomerase"
                     /protein_id="HDI6043522.1"
                     /translation="MVDKRESYTKEDLLASGRGELFGAKGPQLPAPNMLMMDRVVKMT
                     ETGGNFDKGYVEAELDINPDLWFFGCHFIGDPVMPGCLGLDAMWQLVGFYLGWLGGEG
                     KGRALGVGEVKFTGQVLPTAKKVTYRIHFKRIVNRRLIMGLADGEVLVDGRLIYTASD
                     LKVGLFQDTSAF"
     gene            complement(15398..17158)
                     /locus_tag="PN977_003521"
     CDS             complement(15398..17158)
                     /locus_tag="PN977_003521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lon protease family protein"
                     /protein_id="HDI6043523.1"
                     /translation="MTITKLAWRDLVPDTDSYQEIFAQPHLIDENDPLFSDTQPRLQF
                     ALEQLLHTRASSSFMLAKAPEESEYLNLIADAARALQSDAGQLVGCHYEVSGHTIRLR
                     NAVSADDNFATLTQVVAADWVEAEQLFGCLRQFNGDITLQPGLVHQANGGILIISLRT
                     LLAQPLLWMRLKNIVNRERFDWVAFDESRPLPVSVPSMPLKLKVILVGERESLADFQE
                     MEPELSEQAIYSEFEDTLQIVDAESVSQWCRWVTLTARHNHLPAPGADAWPVLIREAA
                     RYTGEQETLPLSPQWILRQCKEVASLCDGDTFSGEQLNLMLQQREWREGFLAERMQDE
                     ILQEQILIETEGERIGQINALSVIEFPGHPRAFGEPSRISCVVHIGDGEFTDIERKAE
                     LGGNIHAKGMMIMQAFLMSELQLEQQIPFSASLTFEQSYSEVDGDSASMAELCALISA
                     LADVPVNQSIAITGSVDQFGRAQPVGGLNEKIEGFFAICQQRELTGKQGVIIPTANVR
                     HLSLHSELVKAVEEGKFTIWAVDDVTDALPLLLNLVWDGEGQTTLMQTIQERIAQASQ
                     QEGRHRFPWPLRWLNWFIPN"
     gene            17344..17796
                     /gene="matP"
                     /locus_tag="PN977_003522"
     CDS             17344..17796
                     /gene="matP"
                     /locus_tag="PN977_003522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ter protein MatP"
                     /protein_id="HDI6043524.1"
                     /translation="MKYQQLENLESGWKWKYLVKKHREGELITRYIEASAAQEAVDVL
                     LSLENEPVLVNNWIDKHMNPELVNRMKQTIRARRKRHFNAEHQHTRKKSIDLEFIVWQ
                     RLAGLAQRRGKTLSETIVQLIEDAENKEKYANKMSSLKQDLQALLGKE"
BASE COUNT         4534 a         4201 c         4731 g         4361 t
ORIGIN      
        1 gtaatgatag ctataccagg aaaaaaaatt acgcctggat gacctctaat accagtatcg
       61 ataacgaagg gcacactaca caaaacctgg gtttaacgga gacactactc gatgacggta
      121 atctgagcta cagcgtgcaa cagggatata acagcgaggg gaaaacggct aatggtagcg
      181 ccagcatgga ctacaaaggg gcgtttgcag atgctcgagt gggctacaac tacagcgata
      241 acggcagtca acaacaactg aactacgctc tttcaggcag tttagttgcc cattcgcagg
      301 gtattacgtt gggtcaatca ttgggtgaaa ctaatgtcct gattgccgcg ccaggcgccg
      361 aaaatactcg tgtggcgaac agcaccgggc tgaaaactga ctggcgtgga tataccgttg
      421 tgccttatgc cacttcttat cgggaaaatc gaattgcact tgatgcggcg tcgttaaaac
      481 gtaacgtgga tcttgaaaat gccgtagtaa acgtggttcc caccaaaggg gcattggttc
      541 tggcggagtt caatgcccat gcgggggcta gggtattaat gaaaacatca aagcagggta
      601 tgccgctgag atttggtgca atggcaacac tggatggcgc acaaacaatt agcggtatca
      661 ttgatgatga tggttcgctc tatatgtctg gtttgccggc gaagggaacg ataactgtac
      721 gctggggcga cgctcccgat caaatttgtc atatcagtta cgagcttacc gaacaacaaa
      781 ttaacgctgc gattacgcgg atggattcag tatgcgaata atttcagggg atagtagtgt
      841 gttaagactc cggcttcttt tgactgctgt tttaatgctg tggggatttc atgccgccgc
      901 ttataccggg cagtgtcata ccactcaggg gaatccgtat attggcgtca attttggcgt
      961 taaaaccctg gaggaggaag agaatacggc tggaaaagtc aaagatgaat tttatcagtg
     1021 gagtgaatcg aatgattatt atgtttcctg cgattgcgat aaagacaatg tcagaagtgg
     1081 tctatgggca ttcgctgcgg attctccgtt agtttatttg ggcgacaact ggtacaaaat
     1141 taacgattat cttgccgcaa aggtgctatt aggagtgaaa ggtaacacac cttcggccgt
     1201 accttttgaa aatctcggta caggaagtga tacccgatgg catatttgcg atcccggtgg
     1261 tcaacgtttg ggcggcaagg gggcaagcgg caatagcgga agtttttcac tgaaaatatt
     1321 gcagccgttt gtcggttcgg tcgtcattcc tcctatggcg ctggcgcgac tatatgagtg
     1381 ttacaacata cccgcaagtg attcctgcac gactacaggt acaccggttt tagtgtatta
     1441 cctgtctggt acgattaatt cacttggctc atgttctgtc aattctggag agacaataga
     1501 ggttgatttg ggggatgtat ttgctgccaa tttccgtgtt gtagggcata aaccgcttgg
     1561 atccagaacg gcagaactct caattccagt caggtgtaat accggaaacg cagagctggt
     1621 taatgttaat ttgagtctga cggcaaccag tgatcccgat tatcctcagg caatcaagac
     1681 atcacgacca ggtgtcggtg ttgtggtcac cgacagccaa aacaacatta tttcgcctgc
     1741 cggtggcaca ctgccgctat ccatccctga cgacgcggac agcttcgctc gaatgaatgt
     1801 ttatccggtc agcacgacag gtgtaccacc ggaaaccggg agctttgaag ccacagcaac
     1861 ggtgagaata aactttgact gatatgtgaa cgtatgaaaa agaacacaat atttcaagtc
     1921 gttatcttat cattcacttt gctttatatg catgtcggga tagctggacc tgaacaagtc
     1981 agtatgcata tttatggtaa tgtggtcgat cagggctgtg atgttgccac caaaagtgca
     2041 ttacaaaata ttcatattgg tgattttaat atcagtgatt ttcagaccgc gaataccgta
     2101 agcactgctg ctgatttgaa tatcgatatc accggttgtg ccgctggtat tactggcgcg
     2161 gacgtccttt ttagcggcga ggctgacacc cttgtgccga cactgctcaa attaactgac
     2221 acaggcggaa gcggtggtat ggcaacgggg attgctgtgc aaattcttga tgcgcaaagt
     2281 cagcaagaaa tcccgctcaa tcaggtccta cctcttacgc ccttaaaagc cggggataac
     2341 acactcaaat atcaacttcg ttataagtcc acaaaggcgg gagcaacggg cggtaatgcg
     2401 acggcggttc tctattttga tctggtttac cagtgaagga gtgatgttga aacagataat
     2461 ctggattctg ttcttactgg gattaacggg gggctgtgag ctatttgccc atgatggcac
     2521 ggtcaacatt agcggatcgt ttcgccgtaa tacatgcgtg ctggcacagg atagcaagca
     2581 aatcaatgtg cagttaggcg acgtctcgct gacacgtttt tctcatggga attatggccc
     2641 ggagaaatcg ttcatcatta atttgcagga ttgcggaaca gatgtcagca cggttgacgt
     2701 caccttttca ggaacccccg acggagtaca gagcgaaatg ctgtccattg aaagtggtac
     2761 ggatgcagcc agtgggctgg caattgcaat tctggatgat gcgaagatat tgattccgct
     2821 taaccaggcg agtaaagact acagcctaca tagcggtaag gtgccattaa ctttttacgc
     2881 gcaattgcga cctgtcaata gcgatgtgca gtcgggtaaa gtgaatgcca gcgcgacatt
     2941 tgtacttcat tatgactaat acgtggaaca gattggcgct cttgattttc gccgttttat
     3001 cgctgctggt ggcgagtgaa ttgcaggcag gagtggtggt cggcggaacg cgatttatct
     3061 ttccggcaga cagagattcg atatctattt tattgactaa taccagtcag gaatcctggc
     3121 ttattaacag taaaatcaat cgcccaacgc gttgggctgg gggtgaagcg tcgacagtac
     3181 cagcaccatt actggccgct ccgccactta ttctcctgaa gcccggtact acaggcacat
     3241 tgcgcttgct gagaacggaa agcgacatct tgcccgtgga tcgcgaaacg ctatttgagt
     3301 taagcattgc cagcgtgcca tccggcaaag ttgaaaatca gagcgtaaaa gtggcgatgc
     3361 gctcggtatt taaactgttc tggcgacccg aaggtttgcc gggcgacccg ctggaagctt
     3421 atcaacaatt acgctggaca cggaattcgc agggtgtaca actcactaac ccaacgcctt
     3481 actacattaa cctgattcag gtgagcgtaa atggtaaagc gttaagtaat gcgggagttg
     3541 tgccacctaa aagccagcgt cagacaagct ggtgtcaggc tataacaccg tgtcatgtct
     3601 cctggcggac gataaatgat tacggcgggt tgagtgcaaa gaaggagcaa aatctgccct
     3661 gaaacaggct cggaaaacgt ttgcgctttt tttgccacag gtcaattccc ttttggtccg
     3721 aactcgcaca taatacgccc ccggtttgca caccgggaat ccaggagagt tcatgtacta
     3781 ccccttcgtt cgtaaagccc ttttccagct cgatccagag cgcgctcatg agtttacttt
     3841 tcagcaatta cgccgtatta ccggaacgcc gtttgaagca ctggtgcggc agaaagtgcc
     3901 tgcgaaacct gttaactgca tgggcctgac gtttaaaaat ccgcttggtc tggcagccgg
     3961 tcttgataaa gacggggagt gcattgacgc gttaggcgcg atgggttttg gatctatcga
     4021 gatcggtacc gtcacgccac gtccacagcc aggtaatgac aagccgcgtc tctttcgtct
     4081 ggtagatgcc gaaggtttga tcaaccgtat gggctttaat aatcttggcg ttgataacct
     4141 cgtagagaac gtaaaaaaag cccattatga tggcgtcctg ggtattaaca tcggcaaaaa
     4201 taaagatacg ccggtggagc agggtaaaga tgactatctg atttgtatgg aaaaaatcta
     4261 tgcttatgcg ggatatatcg ccatcaatat ttcatcgccg aataccccag gactacgcac
     4321 actgcaatat ggcgaagcgc tggatgatct cttaaccgcg attaaaaata agcaaaatga
     4381 tttgcaagcg atgcaccata aatatgtgcc gatcgcagtg aagatcgcgc cggatctttc
     4441 tgaagaagaa ttgatccagg ttgccgatag tttagttcgc cataatattg atggcgttat
     4501 tgcaaccaat accacgctcg atcgttctct ggttcaggga atgaaaaatt gtgatcaaac
     4561 cggtggctta agtggtcgtc cgcttcagtt aaaaagcaca gaaattattc gccgcttgtc
     4621 acaggaatta aacggtcgct taccgatcat cggtgttggc ggcattgact cggttatcgc
     4681 tgcgcgtgaa aagattgctg cgggggcctc actggtgcaa atttattctg gttttatttt
     4741 taaaggtccg ccgctgatta aagaaatcgt tacccatatc taattatttc ttcgaattcc
     4801 tctttataag ataatcaggg ctttatttta agccctggtt gttttatatt catcactgtt
     4861 gcttatttag acattttgta cttttattgt tgaggttatt aagcgaagcg acaatggatt
     4921 gtgttgctgc ggttttatag ggtaggggag aggcaaatgc gaattaaacc agacgataac
     4981 tggcgttggt attacgatga agagcacgat cgaatgatgc tcgatttagc caatggtatg
     5041 ctatttcgct cacgttttgc gcgcaaaatg ttgactccag atgccttttc tcccgcaggc
     5101 ttttgcgttg acgacgccgc gctctatttc tcatttgaag aaaaatgccg cgattttaat
     5161 ttatctaaag aacaaaaagc cgaactggtg cttaatgcgc tggtagcaat tcgttatctc
     5221 aaaccgcaaa tgccgaaaag ctggcatttt gtttcccatg gtgaaatgtg ggtgcccatg
     5281 ccgggcgatg cggcctgcgt ttggttgagc gacactcacg agcaagtcaa tttgctggtc
     5341 gttgaatctg gcgaaaatgc cgcactatgc ctgctggcac aaccctgcgt tgttatagcg
     5401 ggtcgcgcga tgcagctggg cgacgccatc aaaatcatga acgacaggct gaaaccgcag
     5461 gttaatgttg acagcttcag cctcgaacag gcagtttaac gcgtcagctt aagtgcagtc
     5521 ttcggtacac aactacagca aagaatagtg ccatcatcac ccattgctga ttttttcagc
     5581 ggcgtgactt cgccttctaa aagctgaaca cggcaacttc cacaaatgcc cgcgcggcaa
     5641 gaataaggga tacgaattcc ctgattttct aattgctcca gcagcacctg ttggttattt
     5701 ccacgaaatg cctgtccctg ccagtcaata tctacgtttg cgtccggttg ttgcgtgatg
     5761 ttggcagtat catcggcggc acctgcgccg taaattttag ctggagccgt tgccagaatt
     5821 tccacctcat cgccaacgcg aatcacgccg ctattacggg caattaaatt ctgaccaaaa
     5881 tcgacatcgc cgttatcctg ggcagtacgg aaagattgca gcgtttttaa tggttcgcct
     5941 gccggatgtt tttgcccttt ttctgggctg acggtggtga aaatacagcg gctacaaggc
     6001 ttaaccacat caaacacgac atcaccaatg cgaatcactt tccagctatc ttcttcccag
     6061 gccgatgcac cggaaaccac cagattgggg cggaattgct ccatttttac actggccgga
     6121 caacgttgtt ggagatcacg taacgaggcc tcgttagcaa gaaggtaagg ataaccatca
     6181 gcaaatgaca ggggtacagt gttgtggcgt ttcacgcgcc gggtcatttg tggccccacc
     6241 cagcgtaatt gcacttcgcg ggagaaaaat ccacttagcc atttgttgat cgcgtctggc
     6301 gcaattcgcg cggtaaaatg tgtgccccaa acttcggttg gtgcgtcttg tgtggcgaaa
     6361 tcagcaaaac gaacatatgc actactgcca tctggtgcgg tgagatgcaa gccatcatgc
     6421 acgggtgaag gagtaaaccg taccattagc ggaaactggc gagcggtaat aaacgtaccg
     6481 tcaggttccg tgatcataaa gatgcgatcg aaggccagac cactgacatc tgccagggta
     6541 tgtgtaagac caatgccgcg catcgattta acaggatgaa taaaaagccg ggttaatgtc
     6601 gccacggtgc ggtcctcaaa tgaaaataag ccctcaactt tatgacatga cgcgcttatt
     6661 agctataatg cgcaacaatt ttcttagagt aaaagtgacg atatgaattc tctgtttgcc
     6721 agtacggccc gtgggctgga agagctgtta aaaactgaac tggaaaacct gggggccgtt
     6781 gaatgccagg tggttcaggg tggggtccat ttcaagggcg acacacggct tgtttaccag
     6841 agcctgatgt ggagccgcct ggcctcgcgt attatgttgc cgctgggcga gtgtaaggtt
     6901 tacagcgatt tagacctcta tctcggtgtt caggcgatca actggacaga gatgtttaat
     6961 cctggcgcga ccttcgctgt ccacttcagt ggtttgaatg acaccatacg caacagtcag
     7021 tacggtgcga tgaaagtgaa agacgcgatc gtcgatgctt tcacgcggaa aaatctgccg
     7081 cgtccaaatg ttgatcgcga tgcgccggat atccgcgtta acgtctggct gcataaagaa
     7141 actgccagta tcgcccttga tctcagtggt gatggtttac atctgcgtgg ctatcgcgat
     7201 cgtgctggta ttgcgccgat caaagaaacc ctggcagccg cgattgttat gcgatccggc
     7261 tggcagccag gaacaccgct gctcgatccg atgtgtggtt ccggtacgtt gctgattgaa
     7321 gcggcgatgc tggcgaccga tcgcgcacca ggcttgcacc gtggacgttg gggctttagc
     7381 ggctgggcac agcatgatga agccatctgg caggaagtga aagcagaagc gcaaactcgc
     7441 gcccgtaaag gcctggcgga gtacagctcc catttctacg gttcggacag cgacgcacgg
     7501 gtgattcaac gtgcgcgcac taacgcccgt cttgcgggga ttggtgaact gatcaccttt
     7561 gaggtgaaag atgtcgcgca actggccaat ccgctgccga aagggccgta cggtacagtg
     7621 ttgagcaacc cgccatacgg tgaacgtctg gacagcgaac cggcgctgat tgcgctgcat
     7681 agtctgctgg gccgaatcat gaaaaaccag ttcggtggct ggaatctctc tttgtttagt
     7741 gcctcgccgg atctgctcag ctgcctgcag ctgcgtgcag acaaacagta caaggcgaaa
     7801 aacggcccgc tggactgcgt acagaaaaac taccatgttg ccgaaagcac accagacagc
     7861 aaaccggcga tggcagcgga ggactacgcc aaccgtctgc gtaagaacct caaaaaattc
     7921 gagaagtggg ctcgtcagga aggaattgaa tgttaccgcc tgtatgacgc cgatctgccg
     7981 gaatataacg ttgccgttga ccgttatgcc gactgggtgg tggtgcagga gtatgcgccg
     8041 ccaaaaacta ttgatgcgca caaagcgcgt cagcgtctgt tcgatattat cgctgcaacc
     8101 atttcggtac tggggattgc gccaaacaaa ctggtactga aaacccgtga acgccagaag
     8161 ggcaaaaatc agtaccagaa actgggcgag aagggcgagt ttctcgaagt caccgaatat
     8221 aacgctcact tgtgggtgaa cctgacggat tacctcgata ccggtctgtt cctcgatcac
     8281 cgtatcgccc gtcgtatgct cggtcagatg agcaaaggca aagatttcct caacctgttc
     8341 tcttataccg gcagcgccac cgtgcacgcg ggattaggcg gtgcacgcag caccaccacc
     8401 gtggatatgt cgcgtactta tctggagtgg gcagaacgca acctgcgtct gaatggctta
     8461 accgggcgtg cgcatcgcct gattcaggcc gattgcctgg cgtggttgcg tgaggcaaat
     8521 gaacagttcg atctgatctt tatcgatccg ccaacctttt ctaactcaaa acgaatggaa
     8581 gatgcgtttg atgttcagcg cgatcatctg gtgctgatga aagatttgaa acgtctgctg
     8641 cgtgcaggtg ggacgatcat gttctcgaac aacaaacgcg gcttccgtat ggatctcgac
     8701 ggcctggcga aactgggact gaaagcacaa gaaattacgc aaaaaacgct ctcccaggat
     8761 ttcgcccgta accgccagat ccacaactgc tggctgatta ccgcagcctg aaaggaatag
     8821 taatgtcatt aatcagtatg catggcgcat ggctgtcgtt cagcgacgcg ccgcttctcg
     8881 ataacgcaga actgcatatc gaagataacg aacgtgtttg tctggtaggt cgtaacggcg
     8941 caggcaaatc gacgttaatg aagatcctca accgtgaaca ggggctggat gacggtcgca
     9001 ttatttatga gcaagatttg attgtagctc gtctgcaaca ggatccgccg cgtaacgttg
     9061 agggtagcgt ttatgatttc gttgccgaag gcattgaaga acaagcggaa tatctgaaac
     9121 gctatcacga tctttcgcgc ctggtgatga acgacccgag cgagaaaaat ctcaacgaac
     9181 tggcgaaggt tcaggaacag ctggatcacc acaacctgtg gcagctggaa aaccgcatca
     9241 acgaagtgct ggcgcaactg ggattagatc ctaatgttgc gctgtcgtcg ctttccggcg
     9301 gctggttgcg taaagcggca ttaggacgcg cgctggtgag caatccgcgt gtattgctgc
     9361 tcgacgaacc gactaaccac ctggatattg aaaccatcga ctggctggaa gggttcctca
     9421 aaaccttcaa tgggacaatt attttcattt cccacgaccg ttcgtttatc cgcaatatgg
     9481 cgacgcgcat tgttgatctc gatcgcggca agctggtgac ctatccaggg aattacgacc
     9541 agtacctgct ggaaaaagaa gaagccctgc gcgtggaaga attacaaaat gccgagttcg
     9601 atcgcaaact ggcgcaggaa gaggtgtgga tccgccaggg gatcaaagcg cgccgtaccc
     9661 gtaatgaagg ccgcgtacgc gccctgaaag caatgcgccg tgaacgtagc cagcgccgtg
     9721 aggtcatggg gacggcaaag atgcaggtgg aagaagccag ccgctccgga aagatcgttt
     9781 tcgaaatgga agacgtttgc taccaggttg acggtaagca actggtgaaa gatttttctg
     9841 cccaggttct acgtggcgac aaaattgccc tgattggccc gaatgggtgc ggcaaaacca
     9901 cgctgctgaa actgatgctc ggtcagcttc aggcggacag cgggcgtatt cacgttggca
     9961 ccaaactgga agtggcttat ttcgatcagc accgtgcgga actggatccc gataaaacgg
    10021 tgatggataa ccttgccgaa ggtaagcaag aggtgatggt taacggcaag ccacgccacg
    10081 tattgggcta tttgcaggac ttcctgttcc atccgaaacg ggcgatgacg ccggtacgtg
    10141 cgctttctgg cggtgagcgg aaccgcttgc tgctggcgcg tttgttcctc aaaccaagca
    10201 acttattgat tcttgacgaa ccgaccaacg atcttgatgt cgaaacgctg gaactgctgg
    10261 aagaactgat cgacagctac cagggcacgg tattgctggt aagccacgat cgtcagtttg
    10321 tcgataacac cgttacagaa tgctggatct tcgaaggcgg cggtaaaatt ggtcgttatg
    10381 tcggcggtta tcatgatgcc cgtggtcagc aagagcagta tgtggcgctc aaacagcctg
    10441 cggtgaaaaa aaccgaagaa gccgccgcgg caaaagcaga aactgtaaaa cgcagcagta
    10501 gcaaactaag ctataaattg cagcgcgaac tggagcagct accgcaattg cttgaagatc
    10561 tggaggcgaa gctggaagcc ctacagacgc aggtggcgga tgcttccttc ttcagtcagc
    10621 cccatgagca gacacaaaaa gtgctcgctg atatggctgc tgcagagcag gagctggagc
    10681 aagcctttga acgctgggag tatcttgaag cgttaaaaaa tggtggctga tcgcaaaata
    10741 gtcgatttaa acagagacag ctgacaacgg taaaattgcc tgatgaactg ggtttatcag
    10801 gcatgaaaac caacactttg ttcgtcatct gtaaaatagc gcatcattaa ggagtaccaa
    10861 tgtgcgaaca tcatcatgcc gcgaagcaca tcctgtgctc gcagtgtgac atgctggtgg
    10921 cgttaccgcg ccttgagcat ggtcagaaag cggcatgtcc ccggtgtggc acaacgttaa
    10981 ccgtggcgtg ggatgcccct cggcagcgtc ccaccgccta tgcgttggct gcactgttca
    11041 tgctgttgct gtccaacttg tttccttttg tgaatatgaa cgttgcggga gtcaccagtg
    11101 aaattacatt actggaaatt cccggcgtgc ttttttctga ggactacgcc agcctcggca
    11161 cctttttcct gttgtttgtg caactggttc ccgcgttttg tctgataacc attctgttac
    11221 tggtgaatcg cgcggaatta ccggtccgtt taaaagagca actggcacgg gtgctttttc
    11281 aactcaaaac ctggggaatg gcggaaattt tcctcgcggg agtgctggtc agtttcgtta
    11341 aactgatggc ctacggcagc attggtgtcg gcagtagctt tctcccctgg tgtttatttt
    11401 gtgtcctgca actgcgcgcc tttcagtgcg ttgatcgtcg ctggttatgg gacgatatcg
    11461 ccccgatgcc agaactgcgc cagccgctaa aacctggcgt cacggggata cgtcaggggc
    11521 tgcgttcttg ctcctgttgt acggcaatcc ttcctgctga tgaacccgta tgcccgcgct
    11581 gtggtactaa agggtacgtt cggcgtagaa acagcctgca gtggacactc gcgctgcttg
    11641 taacgtccat catgctgtac cttccggcta atattttgcc catcatggtg acggatttat
    11701 taggctcgaa gatgccatcg acgattctcg ctggggtcat cctgttatgg agcgaaggat
    11761 cttatcccgt cgctgcggtt atctttctgg ccagtattat ggtgccaacg ttaaagatga
    11821 tcgccatagc gtggttgtgt tgggatgcca aagggcatgg caagcgcgac agtgaaagaa
    11881 tgcatttgat ttatgaagtt gttgagtttg taggccgctg gtcgatgatt gacgttttcg
    11941 ttatcgcggt gctctcggcg ctggtgcgta tgggaggttt aatgagtatt tatccggcaa
    12001 tgggtgcatt aatgtttgct ttagtcgtca taatgacaat gttttctgct atgacgtttg
    12061 acccgcgttt gtcgtgggat cgtcaacctg aatcagagca tgaggagtcc tgacagtatg
    12121 gaatctaata atggggaagc caaaatccag aaagtgaaga actggtctcc cgtgtggata
    12181 tttcctatcg tcacggcgct cattggggcc tgggttcttt tttatcatta cagccatcag
    12241 gggccggaag tgaccctgat caccgcgaat gcggaaggaa tagaaggtgg taaaaccacc
    12301 attaaaagcc gtagcgttga cgtcggcgtg gttgaaagcg ccacactggc tgatgatttg
    12361 acgcacgttg aaatcaaagc gcggctgaat tccggtatgg aaaaattgct gcataaagat
    12421 accgtctttt gggtggtgaa accgcagatt ggtcgagaag ggattagcgg cctgggaacg
    12481 ctgctgtctg gggtttatat cgaactgcag ccaggcgcaa aaggcagcaa aatggataaa
    12541 tacgatttgc tggactcgcc accgttggcc ccgcctgatg cgaaaggtat ccgtgtgatt
    12601 ctcgatagca aaaaagccgg gcagctctcg ccaggagatc ccgtgctgtt ccgtggctat
    12661 cgggtaggtt cggttgaaac cagcaccttc gatacgcaaa aacgcaatat cagctatcaa
    12721 ctgttcatca atgcacctta tgaccgactg gtgaccagca atgttcgctt ctggaaagat
    12781 agtggcattg cggttgattt gacgtcagcg ggaatgcgtg tggagatggg ctcattgaca
    12841 acgctgctta gtggcggtgt cagctttgat gtgccggaag gtctggattt agggcagcca
    12901 gtggcaccga aaacagcttt cgttttgtat gatgatcaga agagcattca ggattcgttg
    12961 tacaccgatc acattgatta tctgatgttc tttaaagatt cggtacgcgg tctgcaaccg
    13021 ggagctccgg tagaattccg gggtattcgc ctgggtaccg taagcaaagt gccattcttt
    13081 gcgccgaata tgcgtcagac atttaacgat gattaccgta ttccggtact gattcgtatc
    13141 gagccagagc ggctgaaaat gcagcttgga gaaaatgcgg atgttgttga gcaccttggc
    13201 gaattgttga aacgtggttt acgcggatcg ctgaaaaccg gaaacctggt cactggcgca
    13261 ctgtatgttg atctcgattt ctatccaaat acgcctgcaa taaccggtat tcgtgaattt
    13321 aatggttatc agattatccc gaccgttagc ggcggtctgg cgcaaatcca gcaacgactg
    13381 atggaagcgt tggataagat caacaaactg ccattgaatc cgatgattga acaggcaacc
    13441 agtacgcttt ctgaaagtca gcgcacaatg aaaaacctgc aaacgacgct ggatagcatg
    13501 aacaagatcc tcgccagcca gtcgatgcag cagttaccga cggatatgca gtcaacgttg
    13561 cgtgaattga atcgcagtat gcagggcttc cagcctggct ccgcagccta caacaagatg
    13621 gtggcggata tgcagcgact tgatcaggtg ttgcgagaac tgcaaccggt gctgaaaacg
    13681 ctcaacgaga agagtaacgc gctggtattt gaagcgaagg acaaaaaaga tccagagccg
    13741 aagagggcga aacaatgaaa aagtggctag tgacgattgc agcactgtgg ctggccggat
    13801 gcagctccgg cgaaattaat aaaaactatt accagttacc tgtggtgcag ggcggtacac
    13861 aaagtaccgc cagccagggt aatcgcctgt tatgggtaga gcaggtcgct gttcctgact
    13921 atctggcggg aaatggtgtg gtttatcaaa ccagtgatgt gaagtatgtg attgccaaca
    13981 acaacttgtg ggccagcccg ttggatcaac agttgcgcaa cactctggtt gccaacctga
    14041 gcacgcaact gcccggctgg gtggttgcct cccagcctct gggaagcgcc caggatacgc
    14101 tcaatgttac cgtaacggag tttaacgggc gatatgatgg caaggtcatt gtcagtggtg
    14161 aatggctgtt gaaccaccag ggacaactga tcaaacgtcc gttccgtctg gaaggggtgc
    14221 aaactcagga tggttacgat gagatggtta aagtactggc cagtgtctgg agtcaggaag
    14281 ccgcttctat tgcgcaagag ataaagcgtc taccttaatt ataaagattt gtaaatataa
    14341 ccgcctccgg tatgttgcct gaggcggttt ttttgtctct aacgtgcgga aaaagttgtt
    14401 cctcttcaca ttttttgtac aactgacatg cccgtgtagc tcacaaatat gacagtggcg
    14461 tgaattttgc gcattgacgg cagttatgat tcgcggtatt gcttaactgt gattgcacat
    14521 ttagtaatca ctgttttctt ttccaccaga aaccagtatg agggaaacga ggcatgaaga
    14581 gacaaaaacg agatcgcctg gaacgggcac atcaacgtgg ttatcaggcc ggcatcgccg
    14641 gacgctcaaa agaaatgtgt ccctatcaga cgctgaatca aaggtcacaa tggctgggag
    14701 gctggcgaga agccatggcg gacagggtag taatggcctg attctgtctc tttaaaaaga
    14761 aacctccgca ttgcggaggt ttcgcctttt tatactctgt ctgattatta tcagaaggca
    14821 gacgtatcct ggaacagacc gactttcagg tcgctggcgg tatagatcag acgaccatca
    14881 accagcactt cgccatctgc cagacccata atcagacgac gattaacaat gcgtttaaag
    14941 tgaatacggt aggttacttt tttcgctgtc ggcagtacct gaccagtgaa tttcacttcg
    15001 ccaacgccca gcgcgcggcc tttaccttcg ccgcccagcc agccgaggta gaaccctacc
    15061 agctgccaca ttgcgtccag gcccaggcat cccggcataa ccggatcgcc aatgaagtgg
    15121 catccgaaga accacagatc cggattgata tccagctctg cttcaacata ccctttgtcg
    15181 aagttaccac ccgtttcggt cattttgacc acacggtcca tcatcagcat gttcggtgct
    15241 ggcaattgcg ggcctttagc gccaaacagt tcaccgcgac cagaggcaag aaggtcttct
    15301 tttgtatagg attcgcgttt atctaccatg ttctctgtaa gccttatttt attgaagcac
    15361 gcaggatagc taacacgtgt acgctgaaca agtccgatca gttcggaata aaccagttca
    15421 gccaacgtaa tggccatgga aaacggtgac gtccttcctg ttgcgatgct tgtgcgatac
    15481 gttcctggat ggtttgcatc agcgtcgttt ggccttcgcc atcccacacc agatttaata
    15541 ataacggcag tgcgtcagtc acatcgtcta ctgcccagat ggtgaatttg ccttcttcta
    15601 ccgctttcac cagttcactg tgaagactta aatggcgaac gttagcagtg gggataatga
    15661 caccttgttt ccccgttaac tcacgttgct ggcaaatagc aaagaagcct tcgattttct
    15721 catttaaacc accgactggc tgggcgcgac cgaactgatc gactgaacct gtgatagcga
    15781 tactctgatt caccggcaca tcggcgaggg cgctgatcag ggcgcagagt tcagccatcg
    15841 aggcactatc gccatccact tcactgtatg actgctcaaa tgtcagcgat gctgagaagg
    15901 ggatctgttg ctcaagctgt agttccgaca tcaggaacgc ttgcatgatc atcatccctt
    15961 tcgcatggat attgccgcca agctccgctt tgcgttcgat gtcggtgaat tcaccatcgc
    16021 caatatgcac aacgcagcta atgcgagaag gttcgccaaa agcgcgtgga tgacccggaa
    16081 attcaatgac cgaaagggcg ttaatttgcc cgatgcgttc gccttcggtt tcaatcagga
    16141 tttgctcctg aaggatctca tcctgcatgc gttcagcgag gaaaccttca cgccattcac
    16201 gctgctgcag cattaagttt agctgctcgc cggagaaggt gtcgccatcg cacagggagg
    16261 cgacctcttt acactggcgg aggatccact gcgggctaag cggaagtgtt tcttgttcac
    16321 cggtgtagcg ggctgcttcg cggataagta ctggccaggc atccgctccc ggtgcaggta
    16381 agtgattatg tcttgcggtc aatgttaccc agcgacacca ctggcttact gactccgcat
    16441 cgacaatctg cagagtatct tcaaattcgc tataaatagc ctgctctgaa agctctggtt
    16501 ccatctcctg gaaatcagcc aatgattcac gttcgcctac cagaatgact ttcagcttca
    16561 atggcatcga aggcacagag acggggagag ggcgcgactc atcaaacgca acccagtcaa
    16621 aacgctcgcg gttaacgata tttttcagcc gcatccacag cagaggttgc gccagcagtg
    16681 tacgcaaaga gatgatgaga ataccgccat ttgcctgatg caccagacca ggctgcaggg
    16741 taatgtcgcc attaaactgg cgcaggcagc caaagagttg ttccgcttct acccagtcgg
    16801 cagcgacaac ttgcgttaaa gtcgcaaaat tatcatctgc actcactgcg ttacgtaagc
    16861 ggatggtgtg cccggaaacc tcatagtgac agcccaccag ttggcctgca tcgctttgta
    16921 gcgcacgcgc ggcatcggca ataagattca gatactcaga ctcttccggg gccttcgcca
    16981 gcataaaaga ggaggatgct cgcgtatgca gcaactgctc cagcgcaaat tgcagtcgcg
    17041 gttgagtatc actgaataaa ggatcgtttt cgtcaatcaa atgtggctga gcaaatattt
    17101 cctgatagct atcggtatca ggaaccaggt cacgccatgc aagtttcgta atggtcaaag
    17161 ttgatgtttt ttagtctgtt gtcaaagccg cgattatacc gtaaccggca ctgcagcaca
    17221 cgtagaaagc accgacaata ctcctggcat gggcgttaaa gctcacagga tggagattct
    17281 tttcttcact ggcccaaaaa gctgatattc tgtaaagagt tacacggtaa cattgagatc
    17341 gctatgaaat atcaacaact tgaaaatctt gaaagcggtt ggaaatggaa gtatctggtg
    17401 aagaagcatc gcgaagggga gttaatcacc cgttacatag aagccagtgc cgcccaggaa
    17461 gccgtcgatg tgttgctctc gctggaaaat gaacccgtgc tggtaaataa ctggattgat
    17521 aagcatatga atccggaact ggtcaatcgc atgaagcaga ccattcgggc cagacggaag
    17581 cgccatttta atgcggaaca ccagcatacg cgcaaaaaat cgatcgatct ggaatttatt
    17641 gtctggcaac gtctggctgg tcttgcgcag cgtcgcggta aaacgctttc tgaaacgatt
    17701 gttcagctga ttgaagatgc ggaaaacaaa gagaaatacg cgaataaaat gtcttctctg
    17761 aagcaggatc tgcaggcatt gctgggtaag gaataactgt caaaagtcac ttcaatttac
    17821 taaaggc
//
LOCUS       DAMZFT010000100        17766 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.100, whole genome shotgun sequence.
ACCESSION   DAMZFT010000100 DAMZFT010000000
VERSION     DAMZFT010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17766)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17766)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17766
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.100"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(<1..520)
                     /locus_tag="PN977_003523"
     CDS             complement(<1..520)
                     /locus_tag="PN977_003523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HDI6043525.1"
                     /translation="MHHATPLITTIVGGLVLAFILGMLANKLRISPLVGYLLAGVLAG
                     PFTPGFVADTKLAPELAELGVILLMFGVGLHFSLKDLMAVKAIAIPGAIAQIAVATLL
                     GMALSAVLGWSLMTGIVFGLCLSTASTVVLLRALEERQLIDSQRGQIAIGWLIVEDLV
                     MVLTLVLLPAVAG"
     gene            complement(758..1978)
                     /gene="fsr"
                     /locus_tag="PN977_003524"
     CDS             complement(758..1978)
                     /gene="fsr"
                     /locus_tag="PN977_003524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fosmidomycin MFS transporter"
                     /protein_id="HDI6043526.1"
                     /translation="MAMSEQTQPVAGAAASTTKARTSFGILGAISLSHLLNDMIQSLI
                     LAIYPLLQSEFSLTFMQIGMITLTFQLASSLLQPVVGYWTDKYPMPWSLPIGMCFTLS
                     GLVLLALAGSFGAVLLAAALVGTGSSVFHPESSRVARMASGGRHGLAQSIFQVGGNFG
                     SSLGPLLAAVIIAPYGKGNVAWFVLAALLAIVVLAQISRWYSAQHRMNKGKPKATIIN
                     PLPRNKVVLAVSILLILIFSKYFYMASISSYYTFYLMQKFGLSIQNAQLHLFAFLFAV
                     AAGTVIGGPVGDKIGRKYVIWGSILGVAPFTLILPYASLHWTGVLTVIIGFILASAFS
                     AILVYAQELLPGRIGMVSGLFFGFAFGMGGLGAAVLGLIADHTSIELVYKICAFLPLL
                     GMLTIFLPDNRHKD"
     gene            2196..3848
                     /gene="ushA"
                     /locus_tag="PN977_003525"
     CDS             2196..3848
                     /gene="ushA"
                     /locus_tag="PN977_003525"
                     /EC_number="3.1.3.5"
                     /EC_number="3.6.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HDI6043527.1"
                     /translation="MKLLQRGVALALLTTFTLASEAALAYEQDKTYKITVLHTNDHHG
                     HFWRNEYGEYGLAAQKTLVDGIRKEVAAEGGSVLLLSGGDINTGVPESDLQDAEPDFR
                     GMNLVGYDAMAIGNHEFDNPLTVLRQQEKWAKFPLLSANIYQKSTGERLFKPWALFKR
                     QDLKIAVIGLTTDDTAKLGNPENFTDIEFRKPADEAKLVIQELQQTEKPDIIIAATHM
                     GHYDNGEHGSNAPGDVEMARALPAGSLAMIVGGHSQDPVCMAAENKKQVDYVPGTPCK
                     PDQQNGIWIVQAHEWGKYVGRADFEFRNGEMKMVNYQLIPVNLKKKVTWEDGKSERVL
                     YTPEIAENQQMISLLSPFQNKGKAQLEVKIGETNGRLEGDRDKVRFVQTNMGRLILAA
                     QMDRTGADFAVMSGGGIRDSIEAGDISYKNVLKVQPFGNVVVYADMTGKEVIDYLTAV
                     AQMKPDSGAYPQFANVSFVAKGGKLDDLKIKGEPVDPAKTYRMATLNFNATGGDGYPR
                     LDNKPGYVNTGFIDAEVLKAYIQKSSPLDVSVYEPKGEVSWQ"
     gene            complement(3885..4364)
                     /gene="ybaK"
                     /locus_tag="PN977_003526"
     CDS             complement(3885..4364)
                     /gene="ybaK"
                     /locus_tag="PN977_003526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK"
                     /protein_id="HDI6043528.1"
                     /translation="MTPAVKLLEKNKISFQIHTYEHDPAETNFGDEVVKKLGLNPDQV
                     YKTLLVAVNGDMKHLAVAVTPVAGQLDLKKVAKALGAKKVEMADPMVAQRSTGYLVGG
                     ISPLGQKKRLPTIIDAPAQEFATIYVSGGKRGLDIELAAGDLAKILDAKFADIARRD"
     gene            complement(4568..5362)
                     /locus_tag="PN977_003527"
     CDS             complement(4568..5362)
                     /locus_tag="PN977_003527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HDI6043529.1"
                     /translation="MDLLYRVKTLWAALRGNHYTWPAIDISLPGNRHFHLIGSIHMGS
                     HDMAPLPTRLLKKLKNADALIVEADVSTSDTPFANLPACEALEERISEEQLQNLQHIS
                     QEMGISTSLFSTQPLWQIAMVLQATQAQKLGLRAEYGIDYQLLQAAKQQHKPVIELEG
                     AENQIAMLLQLPDKGLALLDDTLTHWHTNARLLQQMMSWWLNAPPQNNEITLPNTFSQ
                     SLYDVLMHQRNLAWRDKLRAMPPGRYVVAVGALHLYGEGNLPQMLR"
     gene            complement(5666..8170)
                     /gene="copA"
                     /locus_tag="PN977_003528"
     CDS             complement(5666..8170)
                     /gene="copA"
                     /locus_tag="PN977_003528"
                     /EC_number="7.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-exporting P-type ATPase CopA"
                     /protein_id="HDI6043530.1"
                     /translation="MSQTIDLTLDGLSCGHCVKRVKESLEQRPDVEQADVSVTEAHVT
                     GTASAEQLIETIKQAGYDASVSHPKAKPLAESSIPSEALTAVSEALPAATADDDDSQQ
                     LLLSGMSCASCVTRVQNALQSVPGVTQARVNLAERTALVMGSASPQDLVQAVEKAGYG
                     AEAIEDDAKRRERQQETAVATMKRFRWQAIVALAVGIPVMVWGMIGDNMMVTADNRSL
                     WLVIGLITLAVMFFAGGHFYRSAWKSLLNGAATMDTLVALGTGVAWLYSMSVNLWPQW
                     FPMEARHLYYEASAMIIGLINLGHMLEARARQRSSKALEKLLDLTPPTARLVTDEGEK
                     NVPLADVQPGMLLRLTTGDRVPVDGEITQGEAWLDEAMLTGEPIPQQKGEGDSVHAGT
                     VVQDGSVLFRASAVGSHTTLSRIIRMVRQAQSSKPEIGQLADKISAVFVPVVVVIALV
                     SAAIWYFFGPAPQIVYTLVIATTVLIIACPCALGLATPMSIISGVGRAAEFGVLVRDA
                     DALQRASTLDTVVFDKTGTLTEGKPQVVAVKTFADVDEAQALRLAAALEQGSSHPLAR
                     AILDKAGDMQLPQVNGFRTLRGLGVSGEAEGHALLLGNQALLNEQQVDTKAIEAEITA
                     QASQGATPVLLAVDGKAVALLAVRDPLRSDSVAALQRLHKAGYRLVMLTGDNPTTANA
                     IAKEAGIDEVIAGVLPDGKAEAIKRLQSEGRQVAMVGDGINDAPALAQADVGIAMGGG
                     SDVAIETAAITLMRHSLMGVADALAISRATLRNMKQNLLGAFIYNSIGIPVAAGILWP
                     FTGTLLNPVVAGAAMALSSITVVSNANRLLRFKPKE"
     gene            8433..9365
                     /gene="glsA"
                     /locus_tag="PN977_003529"
     CDS             8433..9365
                     /gene="glsA"
                     /locus_tag="PN977_003529"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HDI6043531.1"
                     /translation="MLDANKLQQAVDQAYTQFHSLNGGQNADYIPFLANVPGQLAAVA
                     IVTSDGNVYSAGDCDYRFALESISKVCTLALALEDVGPQAVQDKIGADPTGLPFNSVI
                     ALELHGGKPLSPLVNAGAIATTSLINAENAEQRWQRILHIQQQLAGEQVALSDEVNQS
                     EQTTNFHNRAIAWLLYSAGYLYCDAMEACDVYTRQCSTLINTVELATLGATLAAGGVN
                     PLTHKRVLQADNVPYILAEMMMEGLYGRSGDWAYRVGLPGKSGVGGGILAVVPGVMGI
                     AAFSPPLDEEGNSVRGQKMVASVAKQLGYNVFKG"
     gene            9368..10660
                     /locus_tag="PN977_003530"
     CDS             9368..10660
                     /locus_tag="PN977_003530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDI6043532.1"
                     /translation="MMNTEGNNGNKPLGLWNVVSIGIGAMVGAGIFALLGQAALLMEA
                     STWVAFAFGGIVAMFSGYAYARLGASYPSNGGIIDFFRRGLGNGVFSLALSLLYLLTL
                     AVSIAMVARAFGAYAVQFLHEGSQEEHLILLYALGIIAVMTLFNSLSNHAVGRLEVIL
                     VGIKMMILLLLIIAGVWSLQPAHISVSAPPSSGAFFSCIGITFLAYAGFGMMANAADK
                     VKDPQIIMPRAFLVAIGVTTLLYISLALVLLSDVSALELEKYADTAVAQAASPLLGHV
                     GYVIVVIGALLATASAINANLFAVFNIMDNMGSERELPKLMNKSLWRQSTWGNIIVVV
                     LIMLMTAALNLGSLASVASATFLICYLAVFVVAIRLRHDIHASLPILIVGTSVMLLVI
                     VGFIYSLWSQGSRALIWIIGAILLSLIVAMVMKRNKTV"
     gene            10785..11192
                     /gene="cueR"
                     /locus_tag="PN977_003531"
     CDS             10785..11192
                     /gene="cueR"
                     /locus_tag="PN977_003531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(I)-responsive transcriptional regulator"
                     /protein_id="HDI6043533.1"
                     /translation="MNISDVAKITGLTSKAIRFYEEKGLVTPPMRSENGYRTYTQQHL
                     NELTLLRQARQVGFNLEESGELVNLFNDPQRHSADVKRRTLEKVAEIERHIEELQSMR
                     NQLLALANACPGDDSADCPIIENLSGCCHHRAG"
     gene            complement(11196..11399)
                     /locus_tag="PN977_003532"
     CDS             complement(11196..11399)
                     /locus_tag="PN977_003532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000664216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6043534.1"
                     /translation="MKEQRKIPLTHIMIIGAFIFAFLQVVLLASLVHAVNVNNEIQEG
                     LFQSGRIMVESLQHILSVQTGIN"
     gene            complement(11396..11818)
                     /locus_tag="PN977_003533"
     CDS             complement(11396..11818)
                     /locus_tag="PN977_003533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6043535.1"
                     /translation="MMNSSIKSFSLLAVILLAGCSSPTSRIADCQAQGVSHDTCYLAE
                     QQRQTAILSASEAQAFKNAEAAQHAQAAKKAIYKGFGMTFRMSSKNFAYLNDSLCAID
                     EDNKDATVYQSGLYNVIVYHHTGKVALMKEGQFVGYLK"
     gene            complement(11872..12888)
                     /locus_tag="PN977_003534"
     CDS             complement(11872..12888)
                     /locus_tag="PN977_003534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YadA family autotransporter adhesin"
                     /protein_id="HDI6043536.1"
                     /translation="MKTVNVALLALIISAISSPVVLAGDTIEAAATELSAINSGMSQS
                     EIEQKITRFLERTDNSPAAYTYLTEHHYIPSETPDTTQTPTVQTDPDAGQKTVAATGD
                     VQTTARYQSMINARQSAVTDAQQTQITEQQAQIVATQKTLAATGDTQNTAHYQEMINA
                     RLAAQNEANQRTTTEQGQKMNALTTDVAAQQQKERAQYDKQMQSLAQKSVQAHEQIES
                     LRQDSAQTQQQLTNTQKRVADNSQQINTLNNHFDSLKNEVEDNRKEANAGTASAIAIA
                     SQPQVKTGDVMMVSAGAGTFNGESAVSVGTSFNAGTHTVLKAGISADTQSDFGAGVGV
                     GYSF"
     gene            13254..13616
                     /locus_tag="PN977_003535"
     CDS             13254..13616
                     /locus_tag="PN977_003535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6043537.1"
                     /translation="MNEFEKIFNEMNLDRALLPILFRSNRSTVWKYLSGDSTAPASAM
                     SLIMLLQLIQKRNPDLLAEWLTLSDFTIPPEVYLDQPDYWKGWVYTQHKVNKNVLEYL
                     KKHYPDEDQKSMGKGREE"
     gene            complement(13719..14177)
                     /locus_tag="PN977_003536"
     CDS             complement(13719..14177)
                     /locus_tag="PN977_003536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HDI6043538.1"
                     /translation="MMELMVVHPHIFWLSLGGLLLAAEMLGGNGYLLWSGVAAVITGL
                     VVWLVPLGWEWQGVMFAILTLLAAWLWWKWLSRRVREQKHSDSHLNQRGQQLIGRRFV
                     LESPLVNGRGHMRVGDSSWPVSASEDLGAGTHVEVIAIEGITLIIRAVIA"
     gene            complement(14174..15091)
                     /locus_tag="PN977_003537"
     CDS             complement(14174..15091)
                     /locus_tag="PN977_003537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH/Band 7/PHB domain protein"
                     /protein_id="HDI6043539.1"
                     /translation="MLIFIPILIFVALVIVGAGVKIVPQGYQWTVERFGRYTKTLQPG
                     LSLVVPFMDRIGRKINMMEQVLDIPSQEVISKDNANVTIDAVCFIQVIDAPRAAYEVS
                     NLELAIINLTMTNIRTVLGSMELDEMLSQRDSINSRLLRIVDEATNPWGIKVTRIEIR
                     DVRPPAELISSMNAQMKAERTKRAYILEAEGIRQAEILKAEGEKQSQILKAEGERQSA
                     FLQAEARERSAEAEARATKMVSEAIASGDIQAVNYFVAQKYTEALQQIGSSSNSKVVM
                     MPLEASSLMGSIAGIAELVKDSANKRTQP"
     gene            15237..15914
                     /gene="fetA"
                     /locus_tag="PN977_003538"
     CDS             15237..15914
                     /gene="fetA"
                     /locus_tag="PN977_003538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter ATP-binding protein FetA"
                     /protein_id="HDI6043540.1"
                     /translation="MQENSPLLQLQNVGYLASDAKILNNINFSLRAGEFKLITGPSGC
                     GKSTLLKIVASLISPTSGTLLFEGEDVSTLKPEIYRQQVSYCAQTPTLFGDTVYDNLI
                     FPWQIRNQQPEPAIFLDFLERFALPDTILTKNIAELSGGEKQRISLIRNLQFMPKVLL
                     LDEITSALDESNKHNVNEMIHRYVHEQNIAVLWVTHDKDEINHADKVITLQPHAGEMQ
                     EARYELA"
     gene            15901..16680
                     /gene="fetB"
                     /locus_tag="PN977_003539"
     CDS             15901..16680
                     /gene="fetB"
                     /locus_tag="PN977_003539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron export ABC transporter permease subunit
                     FetB"
                     /protein_id="HDI6043541.1"
                     /translation="MNSHNITNESLALALMLVVVAILISHKEKLALEKDILWSVGRAI
                     IQLIIVGYVLKYIFSVDDASLTLLMVLFICFNAAWNAQKRSKYIAKAFISSFIAITVG
                     AGITLAVLILSGSIEFIPMQVIPIAGMIAGNAMVAVGLCYNNLGQRVISEQQQIQEKL
                     SLGATPKQASAILIRDSIRAALIPTVDSAKTVGLVSLPGMMSGLIFAGIDPVKAIKYQ
                     IMVTFMLLSTASLSTIIACYLTYRKFYNSRHQLVVTQLKKK"
     gene            complement(16860..17714)
                     /gene="cnoX"
                     /locus_tag="PN977_003540"
     CDS             complement(16860..17714)
                     /gene="cnoX"
                     /locus_tag="PN977_003540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperedoxin"
                     /protein_id="HDI6043542.1"
                     /translation="MSVENIVNINESNLQQVLEQSMTTPVLFYFWSERSQHCLQLTPI
                     LESLAAQYNGQFILAKLDCDAEQMIAAQFGLRAIPTVYLFQNGQPVDGFQGPQPEEAI
                     RALLDKVLPREEELKAQQAMQLMQEGNYTDALPLLKDAWQLSNQNGEIGLLLAETLIA
                     LNRSEDAEAVLKTIPLQDQDTRYQGLVAQIELLKQAADTPEIQQLQQQVAENPEDAAL
                     ATQLALQLHQVGRNEEALELLFGHLRKDLTAADGQTRKTFQEILAALGTGDALASKYR
                     RQLYALLY"
BASE COUNT         4322 a         4547 c         4583 g         4314 t
ORIGIN      
        1 ttcctgccac tgcgggcagc aacaccagcg tcagaaccat taccaggtct tccacaatca
       61 accaaccgat ggcgatttgc ccacgctgac tgtctattaa ttgccgttct tcaagtgcgc
      121 gcagtaacac cacggtactg gcggtggaaa ggcataagcc gaacacgata ccggtcatta
      181 acgaccagcc cagcacggcg gagagcgcca tacccagcag cgtcgccacg gctatctggg
      241 cgatcgcacc gggaatggcg atggccttta ccgccatcaa atccttcagc gaaaagtgca
      301 gacccacgcc aaacatcaac agaatgacgc ccagttcagc cagttccggc gcaagcttgg
      361 tatcggcaac aaagcccgga gtgaatggtc ctgccagcac acccgctaac agatatccca
      421 ccagaggaga aatacgtagt ttattggcca gcatgccgag gataaaggcg agcacaaggc
      481 cgccaacaat ggtggtgata agcggggtgg cgtgatgcat tccgtctcct tttcctggtg
      541 gttattgtcc atttttggcc gggaaaacca aaattacagg taatagttta tgacaatttc
      601 attgatgatg tttatgaata attgttgaat tttgcagaaa aatggaatta gctgcaaaaa
      661 aagcacggat cggaaaactg aaggcggttt aacagaggaa aggcttatgg cgactgtaaa
      721 acgatgcagc caaagtttgc ctttggctgc aatgaaatca gtctttatgc cggttatcag
      781 gcaggaatat ggtcaacatc cccaatagtg gcaggaaagc acagatttta tagactaact
      841 cgatgctggt gtgatcggcg ataagcccca gaactgccgc tcccagacct cccatgccaa
      901 aagcaaaacc gaaaaagagt ccagaaacca taccgatacg tcccggaagc agctcctgag
      961 cgtagaccag aatggcagag aatgccgaag cgaggataaa tccaataatc accgttaaaa
     1021 cccccgtcca gtgcaggctg gcgtagggta aaatcagcgt aaacggcgca acgccgagga
     1081 tagagcccca aatcacatat ttccgcccaa ttttatcccc tacaggcccg ccgatcaccg
     1141 tacctgccgc aacggcaaac aggaaggcaa acagatgaag ctgagcattc tggatagata
     1201 atccgaattt ttgcatcaga taaaaggtgt aatagctgct gatgctcgcc atatagaaat
     1261 atttcgagaa aatgaggatt aacagaatgc tgaccgccag taccactttg ttacgcggca
     1321 gtggattgat aatcgtcgct ttgggttttc ctttattcat tcggtgctgt gccgagtacc
     1381 aacggctgat ttgcgccaac accacgatcg ccagcagtgc cgcaagcaca aaccaggcaa
     1441 cgttgccttt accataaggc gcgataatca ccgccgccag caaggggccc agggaactgc
     1501 caaagttgcc gccgacctga aagatagatt gcgccaggcc atgccgcccg ccggaagcca
     1561 tacgggccac gcgagaagat tccggatgaa agaccgatga accggtaccg accagcgccg
     1621 ccgccagcag aactgcgcca aaactgcccg ccagcgcaag cagcaccaga ccacttaagg
     1681 taaagcacat gccaattggc aacgaccacg gcatcggata tttatcggtc cagtaaccga
     1741 ccaccggttg cagtagcgaa gaggcgagct ggaaggtgag ggttatcatg ccaatctgca
     1801 taaatgtcag agaaaactct gactgaagca gcggataaat cgccagaatc agcgattgga
     1861 tcatgtcgtt cagcagatgt gagaggctga tagcacctaa aataccaaac gatgttcggg
     1921 ccttggtcgt tgacgcagcc gcgcccgcca caggctgggt ttgttcactc attgccatag
     1981 gaaagtcact ttttcagggt tgcgatgtaa agaatgatct tatttgtgat tattacccga
     2041 ctaacatacc tgtatgcgtc gcctgaagga agtctcaacg ctgaatacag aatttctaat
     2101 ctggatgcag atttatcttc accggacgca gacttgtcta tgatgtcgcg tcatactatt
     2161 tttcaacacg ttgaaatcag gtcagggaga gaagtatgaa attattgcag cggggcgtgg
     2221 cgttagcact gttaaccaca tttacactgg cgagtgaagc tgctctggcg tatgagcagg
     2281 ataaaaccta caaaattaca gttctgcata ccaatgatca tcatgggcat ttttggcgca
     2341 atgaatatgg cgaatatggt ctggcggcgc aaaaaacgct ggtagatggt atccgcaaag
     2401 aggttgcggc tgaaggcggt agcgtgctgc tactttccgg tggtgacatt aacactggcg
     2461 tgcccgagtc tgacttacag gatgccgaac ctgattttcg cggcatgaat ctggtgggct
     2521 acgatgcgat ggcaatcggt aatcatgaat ttgataaccc gctcaccgta ttacgccagc
     2581 aggaaaagtg ggcaaagttc ccgttacttt ctgccaatat ctaccagaaa agtaccggcg
     2641 agcgcctgtt taaaccatgg gcgctgttta agcgtcagga tctgaaaatt gccgttattg
     2701 gcctgacgac ggatgataca gcgaagttag gcaacccaga aaacttcacc gatattgagt
     2761 tccgtaagcc cgccgatgaa gcgaagctgg tgattcagga gctgcaacag acagaaaagc
     2821 cagacattat tatcgcggcg acccatatgg ggcattacga caatggtgag cacggctcta
     2881 acgcaccggg cgatgtggag atggcgcgcg cgctgcctgc cggatcgctg gcgatgatcg
     2941 ttggtggtca ctcgcaagat ccggtctgca tggcggcaga aaacaaaaaa caggtcgatt
     3001 acgtgccagg tacgccatgc aaaccggatc aacaaaacgg catctggatt gtgcaggcgc
     3061 atgagtgggg caaatacgtg ggacgggctg attttgagtt tcgtaatggc gaaatgaaaa
     3121 tggttaacta ccagctgatt ccggtgaacc tgaagaagaa agtgacctgg gaagacggga
     3181 aaagcgagcg cgtgctttac actcctgaaa tcgctgaaaa ccagcaaatg atctcgctgt
     3241 tatcgccgtt ccagaacaaa ggcaaagcgc agctggaagt gaaaataggc gaaaccaatg
     3301 gtcgtctgga aggcgatcgt gacaaagtgc gttttgtaca gaccaatatg gggcggttaa
     3361 ttctggcagc ccaaatggat cgcactggtg ctgactttgc ggtgatgagt ggaggcggaa
     3421 ttcgtgattc tatcgaagca ggcgatatca gctataaaaa cgtgctgaaa gtgcagccat
     3481 tcggcaatgt ggtggtgtat gccgacatga ccgggaaaga ggtgattgat tacctgaccg
     3541 ccgtcgcgca gatgaagcca gattcaggtg cctacccgca atttgctaac gttagctttg
     3601 tggcgaaagg cggcaaactg gacgacctta aaatcaaagg cgaaccggtc gatccggcga
     3661 aaacttatcg tatggcgaca ttaaacttca atgccaccgg cggtgatggc tatccgcgcc
     3721 ttgataacaa accgggctat gtgaataccg gctttattga tgccgaagtg cttaaagcgt
     3781 atatccagaa aagctcgccg ctggatgtga gtgtttatga accgaaaggt gaggtgagct
     3841 ggcagtaatc cgaaagtgcc ggatatttgc atccggcaca atgcttaatc gcggcgggcg
     3901 atatcagcaa atttggcatc gaggatcttt gccagatcgc ctgccgccag ttcgatatcc
     3961 agcccgcgct tgccgccgga aacataaata gtggcaaatt cttgtgcggg ggcgtcgata
     4021 atcgttggca gacgtttttt ctgccccagt gggctaatcc ccccaaccag atatcccgtc
     4081 gaacgctgcg caaccatcgg atcggccatc tcaactttct tggcacccag cgcttttgct
     4141 acttttttaa gatccagttg acctgcgacc ggcgtaacgg ccacggcaag gtgtttcata
     4201 tcaccgttca ctgcgaccag cagtgttttg tagacctgat ccggattcaa acctaatttt
     4261 ttgacgactt catcgccaaa attggtttca gccggatcgt gctcgtaggt atggatttga
     4321 aacgaaatct tgtttttttc gagtaattta actgcgggtg tcatggtaat tttcctgcct
     4381 taaacaaaaa aacgaagcaa gcatacgcct gatggcggtc caaaaaaaag agtcatcttg
     4441 cctaagagta ttggcaggat ggtgagattg agcgacaatc gagttacacc gtcgcttaaa
     4501 gtgacggcat aataataaaa aaatgaaatt cctctttgac gggccaatag cgatattggc
     4561 cattttttta gcgcaacatt tgcggcaaat tcccttctcc atacaggtgt agtgcaccga
     4621 ccgcgaccac atatcgcccc ggcggcatgg cgcgtaattt atcccgccag gcgagatttc
     4681 gctgatgcat cagcacatcg tacagcgact gactgaacgt attgggcagc gttatttcat
     4741 tattttgcgg cggtgcattc agccaccagc tcatcatttg ttgcagcaac cgtgcattgg
     4801 tgtgccagtg ggtcagcgta tcgtccagca gtgccagtcc tttgtcaggg agctggagca
     4861 acatggcaat ctggttttct gccccttcca gttcaatcac gggtttatgt tgttgcttcg
     4921 ccgcctgcaa tagctggtaa tcgataccat attctgcccg gagccccagt ttttgtgcct
     4981 gcgtcgcctg aagaaccatc gcgatttgcc acagcggttg ggtagaaaag agtgaggtag
     5041 aaatgcccat ctcctggcta atgtgctgca ggttttggag ttgctcctcg ctaatgcgct
     5101 cttccagcgc ctcgcaggca ggcaaattag caaaaggcgt atcgctggtg gaaacatccg
     5161 cctcgacgat cagcgcatcg gcgtttttga gctttttgag caaacgggtg ggcaggggag
     5221 ccatatcgtg gctacccata tgaatactgc cgatcagatg aaaatggcga ttgccgggaa
     5281 gagagatatc gatagcaggc caggtgtaat gattaccgcg cagcgcggcc caaagcgttt
     5341 ttacccggta caacagatcc atacgacctc cctttgtgaa atatcatgct agcgcgcggt
     5401 gagggatggc gcaaatggag ttgggacgga ttaaatattt ggcacgccgg ggaaaacgtg
     5461 caaaaaggtc gcgtaatgcc aaaacatcgc cgcatccggc atccggcatc cgagcaccat
     5521 tgcctgatgc gacgcttacg cgtcttatca ggcctacaac cctgtgcctg aatcgtaggc
     5581 cggataaggc gtttatgccg catccggcag gcatgcgtcg atgccagatg cgccacccta
     5641 aagcagcgca tccgcaatga catacttatt ccttcggttt aaaccgcagc aaccggttgg
     5701 cgttactcac tacggtaatc gacgagagcg ccattgccgc tccggcaacc accgggttaa
     5761 gcagcgtacc ggtgaacggc cacaaaatac cggcggcgac cggaatacca atgctgttgt
     5821 agataaacgc accgagcagg ttctgcttca tattgcgcag cgttgcgcgg gaaatcgcga
     5881 gcgcatccgc aacacccatc aggctatggc gcatcagggt aatcgccgcg gtttcaatgg
     5941 caacatcact gccgccaccc atcgcaatac cgacatccgc ctgagccagc gctggcgcgt
     6001 cgttaatgcc gtcgcccacc attgccacct gacgtccttc actttgcagg cgtttaatcg
     6061 cttcggcttt accgtccggc agcaccccgg cgatcacctc atcaatcccg gcttctttgg
     6121 cgatcgcatt ggcggtggtt gggttatccc cggtcaacat caccagacga tatcccgctt
     6181 tatgcaggcg ttgcagcgcc gccacgctat cactacgcaa tggatcgcgt acagccagca
     6241 gggctaccgc tttcccgtca accgccagca gcacaggcgt tgccccttgc gatgcctgag
     6301 cagtaatctc cgcttcgata gctttggtat caacctgttg ctcatttaac agcgcctgat
     6361 tgcccagcaa taacgcatga ccttcagctt caccgctcac gcccagcccg cgcaatgtgc
     6421 ggaaaccgtt gacctgcggc agctgcatat cgcctgcttt atcgaggatc gctcgtgcca
     6481 gcggatggct ggaaccttgc tccagtgccg ccgccagacg caatgcctgc gcttcatcaa
     6541 catcagcaaa cgttttcact gcgacaacct gcggtttacc ttcggtcagc gtcccggttt
     6601 tatcgaacac tacggtatcg agcgtactgg cgcgttgcag cgcgtcggcg tcccgtacca
     6661 gcacgccaaa ctcagccgcc cgcccgacgc ccgaaataat cgacatcggc gtcgccagtc
     6721 ccagcgcaca cggacaggca ataatcagca ccgtggtggc aatcaccagg gtatagacaa
     6781 tctgcggtgc cggaccaaag aaataccaga ttgccgcact gacaagcgca ataaccacta
     6841 ctaccggcac aaatacggct gagattttat ccgccagttg gccgatttca ggcttgctgc
     6901 tctgggcctg gcgcaccatg cgaatgatcc gtgacagcgt agtatggctg ccgaccgcac
     6961 tggcacgaaa cagcacactg ccgtcctgta ccactgtccc ggcatggacg ctatcgcctt
     7021 cgcctttttg ctgcgggata ggttcgcccg tcagcatcgc ttcatccagc cacgcttcgc
     7081 cttgagtaat ctcaccatct accggcacac ggtcgccggt cgtcaggcgc agtaacatac
     7141 ctggctgaac atctgccagc ggcacgtttt tttcaccttc gtcggtgacc aggcgcgccg
     7201 tcggtggggt taaatcgagt aatttctcca gcgccttaga agaacgctga cgtgcgcgcg
     7261 cttccagcat atggccgaga ttgatcagac cgataatcat cgcactggct tcatagtaaa
     7321 gatgtcgcgc ttccatcggg aaccactgcg gccacaggtt gacgctcatc gaatagagcc
     7381 acgccacgcc agtacccagc gccaccagcg tatccatcgt cgctgcaccg ttcagcaggc
     7441 ttttccatgc gctgcggtaa aaatgcccgc cggcgaaaaa cattaccgcc agggttatca
     7501 ggccgataac caaccacagg ctgcggttgt cggcggtgac catcatgtta tcgccgatca
     7561 tcccccagac catcaccggg atacccaccg ccagtgcgac aatcgcctgc cagcggaagc
     7621 gcttcatcgt agcgacggcg gtttcttgct ggcgctcacg gcgtttcgcg tcatcttcaa
     7681 tcgcttccgc accgtagccc gctttttcca ccgcctgcac taaatcttgt ggggaggcac
     7741 tgcccatcac cagcgcagta cgctccgcca ggtttacccg tgcctgagtg acgcccggta
     7801 cgctttgcag cgcattttgt acgcgggtga cacagctggc gcagctcatg ccgctcagca
     7861 gcaactgctg gctgtcatcg tcatcggcgg tcgctgccgg aagcgcctca gaaaccgctg
     7921 tcagtgcttc cgacgggatt gatgactccg ccagcggttt agcctttggg tggcttacag
     7981 atgcgtcata acccgcttgt ttgatggttt cgatcagctg ttctgcactg gcagtcccgg
     8041 taacgtgcgc ttcagtgaca gacacatccg cctgctcaac atccgggcgc tgttcgagac
     8101 tttctttcac gcgtttaacg cagtgaccgc aggacaggcc gtccagggtc aggtcgatag
     8161 tttgtgacat aaaacactcc ttttaagaca gttttgactg gctgtgataa aggttaaacc
     8221 ttccagcaag gggaaggtca agaaattaat aaaccaggcg ggtaaaagtc cgtaaagatt
     8281 aaaaaatcgg ctcgatttgc atcaggatta gacatttatc tctttgtttt cctgtagtta
     8341 agttgcgggt gctaagttaa atcaggatgc ctgaaaatcg gcaccggggt gaggaattac
     8401 ctcccgcatc tataaaaagg agttaacaaa agatgttaga tgcaaacaaa ttacagcagg
     8461 cagtggatca ggcttacacc caatttcact cacttaacgg cggacaaaat gccgattaca
     8521 ttccctttct ggcgaatgta cctggtcaac tggcggcagt ggctatcgtg accagcgatg
     8581 gcaacgtcta tagtgcgggt gactgtgatt accgctttgc gctggaatcc atctcgaaag
     8641 tctgtacgtt agcccttgcg ttagaagatg tcggcccgca ggcggtacag gacaaaattg
     8701 gcgctgaccc gaccggattg ccctttaatt cagttatcgc cttagagttg catggcggca
     8761 aaccgctgtc gccactggta aatgctggcg ctattgccac caccagcctg attaacgctg
     8821 aaaatgccga acaacgctgg cagcgaattt tacatatcca acaacaactg gctggtgagc
     8881 aggtagcgct ctctgatgaa gtcaaccagt cggaacaaac aaccaacttc cataaccggg
     8941 ccattgcctg gctgctgtac tccgccggat atctctattg tgatgcaatg gaagcctgtg
     9001 acgtgtacac ccgtcagtgc tccacgctca tcaatactgt tgaactggca acgcttggcg
     9061 cgacgctggc ggcgggggga gtgaatccgc tgacgcataa acgcgttctt caggccgaca
     9121 acgtgccgta cattctggcc gaaatgatga tggaagggct gtatggtcgc tccggtgact
     9181 gggcgtatcg cgttggttta ccgggcaaaa gcggtgtagg tggcggtatt ctggcggtcg
     9241 tccctggcgt gatgggaatt gccgcgttct caccaccgct ggacgaagaa ggcaacagtg
     9301 ttcgcggtca aaaaatggtg gcatcggtcg ctaagcaact cggctataac gtgtttaagg
     9361 gctgatcatg atgaacacgg aaggtaataa cggtaacaaa cccctcggtc tatggaacgt
     9421 cgtttccatc ggcattgggg caatggtggg ggcggggatc ttcgcgctgc tggggcaggc
     9481 tgcgttgcta atggaagcct cgacctgggt cgcctttgct tttggcggta ttgtggcgat
     9541 gttttccggt tatgcctatg cgcgcctggg ggcgagctat cccagcaatg gcggtattat
     9601 cgacttcttt cgtcgcggat taggcaacgg cgtcttttcg ctggcactct cgttattgta
     9661 cctgttgacg ctggcggtga gcatcgccat ggtcgcccgt gcttttggcg cttatgccgt
     9721 gcagtttttg catgaaggca gtcaggagga gcaccttata ttgctctacg cgttggggat
     9781 cattgcggtg atgacgcttt tcaactcctt aagcaaccat gcggtagggc ggctggaagt
     9841 gatcctcgtc ggcattaaaa tgatgatcct gttattgctg atcattgcag gtgtctggtc
     9901 gctgcaaccg gcacatattt ccgtctctgc gccccccagc tccggtgcgt tcttctcctg
     9961 tattgggata accttccttg cctatgcggg ctttggcatg atggcgaacg cggcggataa
    10021 agtgaaagat ccgcagatca ttatgccacg ggcgtttctg gtggcgattg gcgttaccac
    10081 gttgctttat atctcgctgg cgctggtttt gcttagcgat gtatcggcat tagagttaga
    10141 aaaatatgcc gataccgccg tagcgcaggc tgcttctccg ctgctcgggc atgtgggtta
    10201 tgtgatcgtc gtcatcggcg ctttactggc gacggcttca gccatcaacg cgaacctgtt
    10261 cgccgtgttt aacatcatgg acaacatggg cagcgaacgc gaactgccga agctaatgaa
    10321 taaatccctg tggcggcaga gtacctgggg caacatcatt gtcgtggtgc tgattatgct
    10381 gatgacggcg gcgctgaatt taggctcact cgccagcgtt gccagcgcca cctttttgat
    10441 ctgctatctg gcggtgtttg tggtggcgat ccgcctgcgt catgatattc acgcctcgtt
    10501 gccgattctt atcgttggta cgtcggtgat gttgttggtg atcgttggct ttatctacag
    10561 cctgtggtcc cagggcagcc gtgcgttgat atggattatt ggcgcaatct tactcagcct
    10621 tattgtggca atggtcatga agcgcaataa aaccgtataa cacctctctg tgcgcagtac
    10681 ttcctgtatt attgtggtgg cggtcgatat tcgcactggc aaaaaaacgt gcttgaatat
    10741 ttgttgaaac cctttaacaa agcacaggag gcgttgcgcg aacgatgaac attagcgatg
    10801 tagcaaaaat taccggcctg accagcaaag caatccgctt ctatgaagag aaggggctgg
    10861 tgacgccgcc gatgcgcagc gaaaatggtt atcgaaccta cacgcagcag catctcaacg
    10921 aactgacctt actgcggcag gcacgccagg tgggctttaa cctggaagag agcggcgagc
    10981 tggtgaatct gtttaacgat ccgcagcgtc acagtgccga cgtcaaacgt cgtacgttgg
    11041 agaaggtagc ggagatcgaa cggcatatcg aagaactgca atccatgcgc aaccagctac
    11101 tggcgctggc gaatgcctgc cccggtgatg acagcgccga ctgcccgatt atcgaaaatc
    11161 tctccggctg ctgtcatcat cgggcggggt gaaaatcagt taatccccgt ttgcaccgaa
    11221 agaatatgct gcaaactttc taccataatg cgccccgact gaaataagcc ttcctggatt
    11281 tcgttgttaa cattcacagc gtgaaccagg gaggctaata acaccacttg caagaaggca
    11341 aaaataaacg caccgataat cataatatgc gtcaggggga ttttcctttg ctccttcatt
    11401 ttaaataacc cacaaactga ccttccttca ttaaggcgac ttttcccgtg tggtgataaa
    11461 caatgacgtt ataaagacct gactgataaa cagtggcatc tttattgtct tcatcaattg
    11521 cacataatga atcattgaga taagcaaagt tcttactgct cattctaaag gtcatgccaa
    11581 atcctttata aatggctttc tttgccgcct gggcatgttg tgcggcttct gcatttttaa
    11641 atgcctgcgc ctcggatgca cttaaaatag ccgtctgacg ctgctgttct gcgagataac
    11701 aggtgtcatg gctgacgccc tgcgcctggc aatctgcgat gcgggaagtg ggtgaactac
    11761 agccagccag taatataacc gccagcaggg aaaacgattt aatggagcta ttcatcattg
    11821 ttaaccttaa ataatgtgcg gaaaattgcg aataagaaat tgctgttaaa attagaacga
    11881 atatcccacg ccgacacccg caccgaaatc agattgtgta tccgcagaaa tacctgcttt
    11941 aagtaccgta tgcgttccgg cattaaaaga tgttcctaca gacaccgcag attcaccgtt
    12001 gaacgttccc gctcccgctg acaccatcat cacgtcaccg gttttcacct gtggttgtga
    12061 ggcgatagca atggcagatg cagttcccgc attggcttct ttacgattgt cctcaacttc
    12121 gtttttcaga gaatcgaaat ggttattgag cgtgttaatt tgttggctgt tatctgcgac
    12181 ccgtttttgc gtgttggtta actgttgctg cgtttgtgcg gaatcttgtc tcagactttc
    12241 aatttgctca tgtgcctgga cagacttctg cgccagactt tgcatttgtt tatcgtattg
    12301 agccctttct ttttgctgtt gtgctgccac atcggttgtc agtgcattca ttttctgccc
    12361 ttgttccgtg gtagtgcgct gattagcctc attttgagcc gccagtctgg cattaatcat
    12421 ctcctgataa tgcgcggtat tttgcgtatc tccagtcgcg gcgagcgttt tttgtgtggc
    12481 tacgatctgc gcctgttgct ctgtaatttg cgtttgctgg gcatcagtta ccgcagactg
    12541 tcgggcgttg atcatgctct gataacgggc ggttgtctgt acatcacctg tagcggcaac
    12601 ggttttttgt cctgcgtcag gatctgtctg gacagtggga gtctgagtgg tatcaggtgt
    12661 ttcagaaggg atgtagtgat gttcagtcaa ataggtatac gcagcggggc tgttgtctgt
    12721 gcgttctaaa aagcgggtaa tcttctgctc aatctccgat tgcgacatgc cagagttaat
    12781 ggctgaaagc tctgttgccg ccgcttcaat ggtatcacca gctaaaacaa cagggctgga
    12841 tattgctgaa attatgagtg ccagtaaagc tacgtttaca gttttcattc attttctctt
    12901 gttcaaaata agtcgcatta atgtttctat aagaaacaaa taatattgtt ttaagaaata
    12961 aactgcgttg caatttgatc gaaatctacg ccgccatttg tgtgatgtaa agcgcatttt
    13021 tgtcttgtgt aagcgtaaac accaccaata aggaacaaaa atagtttctt atataaataa
    13081 aaaaggaaat tatttcgtgc tctttcaatg taaaaaagag taaagacttg ttttgataag
    13141 cgaaataaat tctgtttctg gactaaacgt gaagcggttc ctgatgtgtg ctaaaatgcg
    13201 tttctttgat aaatctcaat ttttaagagg tttacgccag gcaggaaatt taaatgaatg
    13261 aatttgagaa gatttttaat gaaatgaatc tggaccgggc attgctaccg atcctttttc
    13321 gttcgaaccg gtcgacagtg tggaagtatt taagcggcga tagtacggct cctgcctctg
    13381 ccatgtcgct gatcatgctg ttgcagttga tccagaaacg aaaccccgat ctgttagccg
    13441 aatggttaac gctttctgat ttcaccattc cgccagaagt ttacctggac caacctgatt
    13501 actggaaagg gtgggtgtat acccaacaca aggtcaataa aaacgtcctg gaatatctaa
    13561 aaaaacacta tccggatgaa gaccaaaaaa gtatgggtaa aggccgggaa gagtaatttt
    13621 gcctctactc ggctatatgc cggatgcggc gcaagcgcct tatctggcct acgattcggg
    13681 catattttgt aggcatgata agacgcgtca gcgtcgcatc aggcgatgac cgcacggatg
    13741 atcagcgtta tcccttctat cgcaatgact tcaacatgcg tacctgcacc gagatcctcg
    13801 ctggcgctga caggccatga actgtcgccg acgcgcatat gaccgcgccc gttgaccagc
    13861 ggagattcca gcacaaaacg tcggccaatc agctgctgcc cgcgctggtt taaatgactg
    13921 tcgctgtgct tttgttcgcg cacccgccgc gacaaccatt tccaccacag ccaggcggcg
    13981 agcagcgtca ggatggcaaa catcacccct tgccactccc aacccagcgg caccagccag
    14041 accaccaggc cggtaatcac cgctgccacg ccgctccaca acaaataacc atttccgccc
    14101 agcatctcgg ctgccagcag caacccgccg agactgagcc agaaaatatg tggatgaacg
    14161 accattaact ccatcatggc tgagtccgtt tgttggcgct gtctttcacc agctcggcaa
    14221 tcccggcaat cgaccccatc aggctgctgg cctctaatgg catcatcact actttgctgt
    14281 tactggagga accgatctgc tgtaacgctt cggtgtattt ctgtgcgacg aagtagttca
    14341 ccgcctgaat atcgccggag gcaatggctt cagacaccat tttggtggcg cgggcttccg
    14401 cttcggcaga acgttcacgc gcttcggcct gtaagaatgc cgactgacgt tcaccttccg
    14461 ctttcaggat ttgcgactgt ttttcacctt cggctttgag gatttccgcc tgacggatcc
    14521 cttccgcttc aaggatgtaa gcgcgtttgg tacgttccgc tttcatctgc gcgttcattg
    14581 aagagataag ctctgccggt gggcgcacgt cgcgaatttc aatacgggtg actttaatcc
    14641 cccacgggtt ggtggcctca tcgacaatac gcagcaggcg tgagttgatg ctgtcgcgct
    14701 gagagagcat ttcatcaagt tccattgaac ccagcacggt acggatgtta gtcatggtca
    14761 ggttgatgat cgccagctcc agattgctga cttcataagc ggcgcgcggg gcatcaatca
    14821 cctgaataaa gcacacggcg tcgatggtaa cgttggcgtt atctttcgag ataacttcct
    14881 gggaagggat gtcgagcact tgctccatca tattgatctt gcgaccaatg cgatccataa
    14941 agggcaccac taggcttaga cctggctgta acgttttggt atagcgacca aaacgctcta
    15001 ccgtccattg atagccctgc ggcacgattt tgacgcccgc gccgacaatg accagcgcga
    15061 caaaaatgag aatagggata aagataagca tcggaaaaac ctcctgttgt accgtccata
    15121 atcagcaaaa ttgctgcttg attaaacaaa ttatacctga ttactgaaag agagttcccc
    15181 cttattcctg cgaaggataa actgttttta gtaaaaatca gaaaaaggga acagtgatgc
    15241 aggaaaatag tcctttgctt cagctacaaa acgtaggata tctggcgagt gatgcgaaga
    15301 ttcttaataa catcaatttt tcgctgcgtg ctggcgaatt taagttaatt accggtcctt
    15361 ctggttgtgg caaaagtacg ctgctaaaaa tagttgcttc attgatcagc ccaaccagcg
    15421 gaacgttact gtttgaaggt gaggatgtca gcacactaaa gccagaaatc taccgccagc
    15481 aagtgtctta ctgcgcccag acgccgacgc tgtttggcga tacggtatac gataatctga
    15541 tctttccctg gcagatccgt aaccagcagc ctgaaccagc catttttctc gattttctcg
    15601 aacgcttcgc tttgccggac accattttga cgaaaaatat cgccgagcta tctggtggtg
    15661 aaaaacaacg catctcattg attcgtaatc ttcaatttat gccgaaggtt ttattgctgg
    15721 atgaaatcac cagtgcgctg gatgaaagta ataaacataa cgttaatgag atgatccatc
    15781 gttatgtgca cgagcaaaat attgccgtgc tgtgggtgac acacgataaa gacgaaatta
    15841 atcatgcgga taaggtgatt acactgcaac cgcatgccgg agaaatgcag gaagcacgct
    15901 atgaactcgc ataatattac taacgaatca ttggcactgg cattaatgct ggtggtggtg
    15961 gcaatcttaa ttagccataa agaaaaactg gcgctggaga aagatattct ctggagcgtc
    16021 gggcgcgcga taattcagct gattattgtc ggctatgtgc tgaagtatat tttcagcgtg
    16081 gatgatgcca gcctgacatt attgatggtg ttatttatct gctttaatgc ggcgtggaat
    16141 gcgcaaaaac gcagtaaata tattgctaaa gcttttatct catcgtttat tgccattacg
    16201 gtcggggcgg gaattaccct ggcggtgctg attctctcag ggtcgattga atttatcccg
    16261 atgcaggtga tccctatcgc cgggatgata gccggtaacg ccatggtagc ggtggggttg
    16321 tgttacaaca atttagggca acgcgtgatt agcgaacagc aacagattca ggagaaactg
    16381 agtcttggcg cgacgccgaa acaggcttca gcgatattga ttcgcgacag tattcgcgcg
    16441 gctttaattc cgacggtcga ttcagcaaaa acggttggct tagtgagttt accaggaatg
    16501 atgtccgggc tgatatttgc cgggattgat ccggtgaagg cgattaaata tcagattatg
    16561 gtgaccttta tgctgctctc aactgccagc ctgtcgacca ttattgcctg ctatttaacc
    16621 tatcgtaaat tttataattc gcgccaccag ttggtggtga cgcaattgaa gaagaaataa
    16681 tgcgatgcct gatgcgtctt atcagaccta catggcacct gcaatgtatt gaattagcac
    16741 ggtttggtag gccggataag gcgttcacgc cgcatccggc ggctatgcgc ggatgcctga
    16801 tgcgacgctg gcgcgtctta tcaggcctac aaaccagcac atcgcatttc aacggaaact
    16861 caatacaaca acgcatacag ctggcggcga tacttcgacg ccagtgcatc acctgtaccc
    16921 agcgcagcga ggatctcctg gaacgtttta cgcgtctgac cgtctgcggc ggtgagatct
    16981 ttacgcagat gcccaaacag taactccagc gcctcttcat tgcgcccaac ctggtgcagt
    17041 tgcagtgcca gttgcgtcgc cagtgcggca tcttctggat tctccgccac ctgctgttgc
    17101 agctgttgaa tttccggcgt atctgccgcc tgcttcagca gttcgatttg cgccaccagc
    17161 ccctggtagc gggtgtcctg atcctgtaaa ggaatggttt tcagcacggc ttcagcatct
    17221 tcagaacggt tcagcgcaat cagcgtttct gccagcagca ggccgatctc cccgttctga
    17281 ttcgacaact gccaggcgtc tttcagcaat ggcaaggcgt cggtgtaatt gccttcctgc
    17341 atcagctgca tcgcctgctg cgctttcagc tcttcttcgc gcggcagcac tttatccagc
    17401 agggcgcgga tcgcctcttc cggttgcggc ccctggaagc cgtctaccgg ttgcccgttc
    17461 tggaacagat acacggtcgg aatcgcgcgc agaccaaact gggcggcaat catctgctcc
    17521 gcgtcgcagt ccagcttcgc cagaataaat tgcccgttgt actgcgccgc gaggctttcc
    17581 agaattgggg ttaactgcaa acagtgctgg ctacgttcag accaaaaata gaacagcacc
    17641 ggagtggtca tcgactgttc aagaacctgt tgcaggttag attcgttaat gttgacaata
    17701 ttttctacgg acatggagtc gctctctgtt gtcgatattt tctttgacat gggggcttaa
    17761 gcgcgc
//
LOCUS       DAMZFT010000101        17598 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.101, whole genome shotgun sequence.
ACCESSION   DAMZFT010000101 DAMZFT010000000
VERSION     DAMZFT010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17598)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17598)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17598
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.101"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..74
                     /locus_tag="PN977_003541"
     CDS             <1..74
                     /locus_tag="PN977_003541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HDI6043543.1"
                     /translation="SFSKSVELHDKVIGHYLNIKHYQ"
     gene            89..325
                     /locus_tag="PN977_003542"
                     /pseudo
     CDS             89..325
                     /locus_tag="PN977_003542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308602.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="uracil/xanthine transporter"
     gene            347..1492
                     /gene="glxK"
                     /locus_tag="PN977_003543"
     CDS             347..1492
                     /gene="glxK"
                     /locus_tag="PN977_003543"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate 3-kinase"
                     /protein_id="HDI6043544.1"
                     /translation="MKIVIAPDSFKESLSAEKCCQAIKAGFSTIFPDAHYICLPIADG
                     GEGTVEAMVAATGGNIVKLEVCGPMGEKVNAFYGLTGDGKTAVIEMAAASGLMLVAPE
                     KRNPLLASSFGTGELIRHALDNGIRHIILGIGGSATVDGGMGMAQALGVRFLDADGQP
                     LAANGGNLARVASIEMNECDPRLANCHIEVACDVDNPLVGARGAAAVFGPQKGATPEM
                     VEELEQGLQNYARVLQQLTEINVCQMAGGGAAGGMGIAAAVFLNADIKPGIEIVLRAV
                     NLEQAVQGAALVITGEGRIDSQTAGGKAPLGVASVAKQFNVPVIGIAGVLGDGVEVVH
                     QYGIDAVFSILPRLAPLAEVLASGETNLFNSARNIACAIKIGQGIKN"
     gene            complement(1622..2407)
                     /gene="allE"
                     /locus_tag="PN977_003544"
     CDS             complement(1622..2407)
                     /gene="allE"
                     /locus_tag="PN977_003544"
                     /EC_number="3.5.3.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(S)-ureidoglycine aminohydrolase"
                     /protein_id="HDI6043545.1"
                     /translation="MGYLNNVTGYRDDLLANRAIVKHGNFALLTPDGLVKNIIPGFEN
                     CDATILSTPKLGASFVDYLVTLHQNGGNQQGFGGEGIETFLYVISGNITAKAEGKTFA
                     LSEGGYLYCPPGSLMTFVNVQAEDSQIFLYKRRYIPVEGHAPWLVSGNASELERIHYE
                     GMDDVILLDFLPKELGFDMNMHILSFAPGASHGYIETHVQEHGAYILSGQGVYNLDNN
                     WIPVKKGDYIFMGAYSLQAGYGVGRGEAFSYIYSKDCNRDVEI"
     gene            complement(2418..3653)
                     /gene="allC"
                     /locus_tag="PN977_003545"
     CDS             complement(2418..3653)
                     /gene="allC"
                     /locus_tag="PN977_003545"
                     /EC_number="3.5.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="allantoate deiminase"
                     /protein_id="HDI6043546.1"
                     /translation="MITHFRQAIEETLPWLSSFGSDPTGGMTRLLYSPEWLETQQQFK
                     KRMAASGLETRFDEVGNLYGRLSGTEYPQEVVLSGSHIDTVVNGGNLDGQFGALAAWL
                     AIDWLKTQYGAPLRTVEVVAMAEEEGSRFPYVFWGSKNIFGLANPDDVRNICDAKGNS
                     FVDAMKACGFTLPNAPLTPRQDIKAFVELHIEQGCVLESNGQSIGVVNAIVGQRRYTV
                     TLNGESNHAGTTPMGYRRDTVYAFSRICHQSIEKAKKMGDPLVLTFGKVEPRPNTVNV
                     VPGKTTFTIDCRHTDAAVLRNFTQQLENDMRAICDEMDIGIDIDLWMDEEPVPMNKEL
                     VATLTELCESEKLNYRVMHSGAGHDAQIFAPRVPTCMIFIPSINGISHNPAERTNITD
                     LAEGVKTLALMLYQLAWQK"
     gene            complement(3675..4724)
                     /gene="allD"
                     /locus_tag="PN977_003546"
     CDS             complement(3675..4724)
                     /gene="allD"
                     /locus_tag="PN977_003546"
                     /EC_number="1.1.1.154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ureidoglycolate dehydrogenase"
                     /protein_id="HDI6043547.1"
                     /translation="MKISRETLHQLIENKLCQAGLKREHAATVAEVLVYADARGIHSH
                     GAVRVEYYAERISKGGTNREPEFRLEETGPCSAILHADNAAGQVAAKMGMEHAIKTAQ
                     QNGVAVVGISRMGHSGAISYFVQQAARAGLIGISMCQSDPMVVPFGGAEIYYGTNPLA
                     FAAPGEGDEILTFDMATTVQAWGKVLDARSRNMSIPDTWAVDKNGAPTTDPFAVHALL
                     PAAGPKGYGLMMMIDVLSGVLLGLPFGRQVSSMYDDLHAGRNLGQLHIVINPNFFSSS
                     ELFRQHLSQTMRELNAITPAPGFNQVYYPGQDQDIKQRKAAVEGIEIVDDIYQYLISD
                     ALYNTSYETKNPFAQ"
     gene            5041..6708
                     /locus_tag="PN977_003547"
     CDS             5041..6708
                     /locus_tag="PN977_003547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase FdrA"
                     /protein_id="HDI6043548.1"
                     /translation="MIHAFIKKGCFQDSVSLMIISRKLSESENVDDVSVMMGTPANKA
                     LLDTTGFWHDDFNHATPNDICVAIRSEAADAGIAQAVMQQLEEALKQLAQGSGSSQAL
                     TQVRRWDSACQKLPDASLALISVAGEYAAELANQALDRNLNVMMFSDNVTLEDEIQLK
                     TRAREKGLLVMGPDCGTSMIAATPLAFANVMPEGNIGVIGASGTGIQELCSQIALAGE
                     GITHAIGLGGRDLSREVGGISALTALEMLSADEKSEVLAFVSKPPAEAVRLKIVNAMK
                     ATGKPTVALFLGYTPAVARDENVWFASSLDEAARLACLLSRVTARRNAIAPVSSGFIC
                     GLYTGGTLAAEAAGLLAGHLGVEADDTHQHGMMLDADGHQILDLGDDFYTVGRPHPMI
                     DPTLRNLLIADLGAKPQVRVLLLDVVIGFGATADPAASLVSAWQKACAARSDNQPLYA
                     IATVTGTERDPQCRAQQIATLEDAGIAVVSSLPEATLLAAALIHPLSPATQQHTPSLL
                     ENVAVINIGLRSFALELQSASKPVVHYQWSPVAGGNKKLARLLERLQ"
     gene            6718..7977
                     /locus_tag="PN977_003548"
     CDS             6718..7977
                     /locus_tag="PN977_003548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009029994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HDI6043549.1"
                     /translation="MFTSVAQANAAVIEQIRRARPHWLDVQPASSLISELNKGKTLLH
                     AGPPMRWQEMTGPMKGACVGACLFEGWAKDEAQALAMLEQGEVNFIPCHHVNAVGPMG
                     GITSASMPMLVVENVTDGNRAYCNLNEGIGKVMRFGAYGEDVLTRHRWMRDVLMPVLS
                     AALGRMERGIDLTAMMAQGITMGDEFHQRNIASSALLMRALAPQIARLNHDKQHIAEV
                     MDFLSVTDQFFLNLAMAYCKAAMDAGAMIRAGSIVTAMTRNGNMFGIRVSGLGERWFT
                     APVNTPQGLFFTGFSQEQANPDMGDSAITETFGIGGAAMIAAPGVTRFVGAGGMEAAR
                     AVSEEMAEIFLERNMQLQIPGWDFQGACLGLDIRRVVETGITPLINTGIAHKEAGIGQ
                     IGAGTVRAPLACFEQALEALAESMGIG"
     gene            7988..8803
                     /locus_tag="PN977_003549"
     CDS             7988..8803
                     /locus_tag="PN977_003549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
                     /protein_id="HDI6043550.1"
                     /translation="MTIIHPLLASRSAPNYCQSWRLAGIWRRAINLMTESGELLTLHR
                     QGSGFGPGGWVLRRAQFDALCGGLCGNERPQVVAQGIRLGRFTVKQPQRYCLLRITPP
                     AHPQPLAAAWMQRAEETGLFGPLAMAASDPLPAELRQFRHCFQAALNGVKTDWRHWLG
                     KGPGLTPSHDDTLSGMLLAAWYYGALDARSGRPFFACSDNLQLVTTAVSVSYLRYAAQ
                     GYFASPLLHFVHALSCPKRTAAAIDSLLALGHTSGADTLLGFWLGQQLLQGKP"
     gene            8800..9693
                     /locus_tag="PN977_003550"
     CDS             8800..9693
                     /locus_tag="PN977_003550"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HDI6043551.1"
                     /translation="MKTLVVALGGNALLQRGEALTAENQYRNIASAVPALARLARSYR
                     LAIVHGNGPQVGLLALQNLAWKEVEPYPLDVLVAESQGMIGYMLVQSLSAQPQMPPVT
                     TVLTRIEVSPDDPAFLQPEKFIGPVYQPEEQEALEAAYGWQMKRDGKYLRRVVASPQP
                     RNILDSEAIELLLKEGHVVICSGGGGVPVTDDGVGSEAVIDKDLAAALLAEQINADGL
                     VILTDADAVYENWGTPQQRAIRHATPDELAPLAKADGSMGPKVTAVSGYVRSRGKPAW
                     IGALSRIEETLAGEAGTCISL"
     gene            complement(9926..10993)
                     /gene="purK"
                     /locus_tag="PN977_003551"
     CDS             complement(9926..10993)
                     /gene="purK"
                     /locus_tag="PN977_003551"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HDI6043552.1"
                     /translation="MKQVCVLGNGQLGRMLRQAGEPLGIAVWPVGLDAEPAAVPFQQS
                     VITAEIERWPETALTRELARHPAFVNRDVFPIIADRLTQKQLFDKLHLPTAPWQLLAD
                     RSEWPAVFDRLGELAIVKRRTGGYDGRGQWRLRADETEQLPTECYGECIVEQGINFSG
                     EVSLVGARGFDGSTVFYPLTHNLHQDGILRTSVAFPQANAQQQAQAEEMLSAIMQELG
                     YVGVMAMECFVTPQGLLINELAPRVHNSGHWTQNGASISQFELHLRAITDLPLPQPVV
                     NSPSVMINLIGSDVNYDWLKLPLVHLHWYDKEVRPGRKVGHLNLTDSDTSRLTATLEA
                     LIPLLPPEYASGVIWAQSKFS"
     gene            complement(10990..11499)
                     /gene="purE"
                     /locus_tag="PN977_003552"
     CDS             complement(10990..11499)
                     /gene="purE"
                     /locus_tag="PN977_003552"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HDI6043553.1"
                     /translation="MSSRNNPARVAIVMGSKSDWATMQFAAEIFEILNVPHHVEVVSA
                     HRTPDKLFSFAESAEENGYQVIIAGAGGAAHLPGMIAAKTLVPVLGVPVQSAALSGVD
                     SLYSIVQMPRGIPVGTLAIGKAGAANAALLAAQILATHDKELHQRLNDWRKAQTDEVL
                     ENPDPRGAA"
     gene            complement(11616..12338)
                     /gene="lpxH"
                     /locus_tag="PN977_003553"
     CDS             complement(11616..12338)
                     /gene="lpxH"
                     /locus_tag="PN977_003553"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HDI6043554.1"
                     /translation="MATLFIADLHLCVEEPAITAGFLRFLAGEARKADALYILGDLFE
                     AWIGDDDPNPLHRQMAAAIKAVSDSGVPCYFIHGNRDFLLGKRFARESGMTLLPEEKV
                     LELYGRRVLIMHGDTLCTDDAGYQAFRAKVHKPWLQTLFLALPLFVRKRIAARMRANS
                     KEANSSKSLAIMDVNQNAVVSAMEKHQVQWLIHGHTHRPAVHELIANQQPAFRVVLGA
                     WHTEGSMVKVTADDVELIHFPF"
     gene            complement(12341..12835)
                     /gene="ppiB"
                     /locus_tag="PN977_003554"
     CDS             complement(12341..12835)
                     /gene="ppiB"
                     /locus_tag="PN977_003554"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017465360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase B"
                     /protein_id="HDI6043555.1"
                     /translation="MVTFHTNHGDIVIKTFDDKAPETVKNFLDYCREGFYNNTIFHRV
                     INGFMIQGGGFEPGMKQKTTKDPIKNEANNGLKNTRGTLAMARTQAPHSATAQFFINV
                     VDNDFLNFSGESLQGWGYCVFAEVVEGMDVVDKIKGVATGRSGMHQDVPKEDVIIESV
                     TVSE"
     gene            13009..14394
                     /gene="cysS"
                     /locus_tag="PN977_003555"
     CDS             13009..14394
                     /gene="cysS"
                     /locus_tag="PN977_003555"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HDI6043556.1"
                     /translation="MLKIFNTLTRQKEEFKPIHAGEVGMYVCGITVYDLCHIGHGRTF
                     VAFDVVARYLRFLGYKLKYVRNITDIDDKIIKRANENGESFVALVDRMIAEMHKDFDA
                     LNILRPDMEPRATHHIAEIIELTEQLIAKGHAYVADNGDVMFDVPTDPTYGVLSRQDL
                     DQLQAGARVDVVDDKRNPMDFVLWKMSKEGEPSWPSPWGAGRPGWHIECSAMNCKQLG
                     NHFDIHGGGSDLMFPHHENEIAQSTCAHDGQYVNYWMHSGMVMVDREKMSKSLGNFFT
                     VRDVLKYYDAETVRYFLMSGHYRSQLNYSEENLKQARAALERLYTALRGTDKTVAPAG
                     GEAFEARFIEAMNDDFNTPEAYSVLFDMAREVNRLKAEDMAAANAMASHLRKLSAVLG
                     LLEQEPEAFLQSGAQADDSEVAEIEALIQQRLDARKAKDWAAADAARDRLNEMGIVLE
                     DGPQGTTWRRK"
     gene            complement(14430..14951)
                     /locus_tag="PN977_003556"
     CDS             complement(14430..14951)
                     /locus_tag="PN977_003556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HDI6043557.1"
                     /translation="MPTVITHAAVPLCIGLGLGSKAIPPRLLFAGIVLAMLPDADVLS
                     FKFGVAYGNIFGHRGFTHSLVFAFVVPLLCVLIGRRWFRAGLIRCWLFLTVSLLSHSL
                     LDSVTTGGKGVGWLWPWSDELFFAPWQVIKVAPFALSRYTTPYGHQVIISELMWVWLP
                     GMLLMGMLWWRRR"
     gene            complement(15059..15271)
                     /gene="ybcJ"
                     /locus_tag="PN977_003557"
     CDS             complement(15059..15271)
                     /gene="ybcJ"
                     /locus_tag="PN977_003557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein YbcJ"
                     /protein_id="HDI6043558.1"
                     /translation="MATFSLGKHPHVELCDLLKLEGWSESGAQAKIAIAEGQVKVDGA
                     VETRKRCKIVAGQTVSFAGHSVQVVA"
     gene            complement(15273..16139)
                     /gene="folD"
                     /locus_tag="PN977_003558"
     CDS             complement(15273..16139)
                     /gene="folD"
                     /locus_tag="PN977_003558"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HDI6043559.1"
                     /translation="MAAKIIDGKTIAQQVRSEVAQKVQARIAAGLRAPGLAVVLVGSN
                     PASQIYVASKRKACEEVGFVSRSYDLPETTSEAELLELIDTLNADNTIDGILVQLPLP
                     AGIDNVKVLERIHPDKDVDGFHPYNVGRLCQRAPRLRPCTPRGIVTLLERYNIDTFGL
                     NAVVIGASNIVGRPMSMELLLAGCTTTVTHRFTKNLRHHVENADLLIVAVGKPGFIPG
                     DWIKEGAIVIDVGINRLENGKVVGDVVFEDAAKRASYITPVPGGVGPMTVATLIENTL
                     QACVEYHDPQGE"
     gene            16611..17153
                     /gene="fimA"
                     /locus_tag="PN977_003559"
     CDS             16611..17153
                     /gene="fimA"
                     /locus_tag="PN977_003559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308619.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HDI6043560.1"
                     /translation="MKLRFISSALAAALFAATGSYAAVVDGGTIHFEGELVNAACSVN
                     TDSADQVVTLGQYRTDIFNAVGNTSALIPFTIQLNDCDPVVAANAAVAFSGQADAIND
                     NLLAIASSTNTTTATGVGIEILDNTSAILKPDGNSFSTNQNLIPGTNVLHFSARYKGT
                     GTSASAGQANADATFIMRYE"
     gene            17372..>17598
                     /locus_tag="PN977_003560"
     CDS             17372..>17598
                     /locus_tag="PN977_003560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HDI6043561.1"
                     /translation="MMTKIKLLMLIIFYLIISTSAHAAGGIALGATRIIYPADAKQTA
                     VWIRNSHTNERFLVNSWIENSSGVKEKSFIIT"
BASE COUNT         4156 a         4475 c         4786 g         4181 t
ORIGIN      
        1 tgtcgttctc aaaatcggtg gagctgcatg acaaggtcat cgggcattat ctgaacataa
       61 aacactatca gtaagttgga gtcattacca tctgccttta ctcttttccg cgctggtgtt
      121 tagccagcaa ataacgttta ccgctcgcaa tatttatcga ctcgcattgc cgttatttgt
      181 cggcatattt ttaatggcat taccgcctgt gtatctgcaa gaccttccat taacgcttcg
      241 tcctctgctc agtaacggct tactggtcgg aattttactg gctgttctta tggataatct
      301 tattccgtgg gaacgcatcg aataatttgt tgaaaaagga ttgataatga agattgtcat
      361 tgcgccagac tcttttaaag agagcttaag tgcagaaaaa tgttgtcagg caattaaagc
      421 cgggttttcg accatatttc ccgatgcgca ctatatctgt ttgccgatag cggatggcgg
      481 cgaagggacg gtggaggcga tggtcgccgc gacgggcggt aacatcgtga agcttgaagt
      541 ctgcgggccg atgggcgaaa aagtgaatgc tttttatgga cttaccggcg acgggaaaac
      601 ggcagtgatt gagatggcgg cagcaagtgg cctgatgctg gtcgcgcctg aaaagcgtaa
      661 tccgttgctg gcctccagtt ttggtacggg ggagttaatt cgccatgcgc tggataacgg
      721 cattcgccat attattctcg gcattggcgg cagtgcgacg gtcgacggcg gtatgggcat
      781 ggcgcaggcg ctcggtgtgc gtttccttga tgccgacggt cagccgctgg cggctaacgg
      841 cggcaattta gcgcgggtgg caagtattga gatgaatgaa tgcgatccgc gtctggcgaa
      901 ctgtcatatt gaagtagcat gtgacgttga taacccgctg gtaggggcac gcggcgcggc
      961 ggcggtgttt ggtccgcaaa aaggggcaac gccggaaatg gtcgaagaac tggaacaggg
     1021 gctgcaaaat tacgcccgtg ttttacaaca gctaactgaa atcaacgtct gccagatggc
     1081 gggtggcggc gcggcgggcg gtatgggcat tgcggcggcg gtattcctca atgcggatat
     1141 taaaccgggt attgagattg tactgcgggc ggtcaacctg gagcaggcgg tgcagggtgc
     1201 agcgctggtg atcaccgggg aagggcgcat cgattcgcaa acggcaggtg gtaaagcgcc
     1261 gctgggcgtg gcgtcggtgg cgaagcagtt taatgtcccg gtgattggga ttgctggcgt
     1321 attgggtgat ggcgtggaag tggtgcacca gtacggcatt gacgcggtgt tcagcatttt
     1381 gccacgtctg gcacctttag ccgaagtgct cgccagcggt gaaaccaatc tcttcaacag
     1441 cgcacgaaat attgcctgcg ccattaaaat aggtcaggga attaaaaact gactattacc
     1501 tttaaagcgg atgcgattta tatcgcatcc gttttttacc tgaatttacc gcaaaaaaag
     1561 ccacggaata aaccgtggca aaatccaaca tagctaaaaa taatcaggcg agtggtatga
     1621 cttaaatctc tacgtcgcgg ttgcaatctt tcgagtaaat atagctgaac gcttcaccac
     1681 gccctacgcc ataaccagcc tgtaaagaat aagcgcccat aaagatgtaa tcgccttttt
     1741 tcaccgggat ccagttattg tcgaggttat aaaccccctg accggaaaga atataggcac
     1801 cgtgttcctg aacgtgggtt tcgatataac cgtggctggc acctggtgca aaagagagga
     1861 tatgcatgtt catatcaaaa cctaactctt tgggcagaaa atccagcaga ataacatcgt
     1921 ccatgccttc ataatgaatg cgttccagtt cgctggcatt gccagaaacc agccacggcg
     1981 catgaccttc taccggaata tagcgacgct tatataaaaa gatttggctg tcttcggcct
     2041 ggacgttaac aaacgtcatt aaggagcctg gcgggcaata aagatagcca ccttcgctta
     2101 aggcaaatgt tttaccttcg gctttggcag tgatatttcc agagatcaca tacaggaacg
     2161 tttcaatgcc ttcgccaccg aagccctgtt ggttgccgcc gttttgatgc agtgtgacca
     2221 gataatcaac aaaagaggca cccagttttg gcgtggagag gattgtcgcg tcacaatttt
     2281 caaagcccgg aataatattt tttaccaggc cgtccggggt taacagcgcg aaattaccgt
     2341 gtttcacaat cgcacggtta gccagtaaat catcgcggta accggtgacg ttatttaaat
     2401 atcccattta tgactcctta tttctgccag gcaagttgat aaagcatgag tgccaacgtt
     2461 ttgacccctt cggcaaggtc ggtaatattg gtgcgttccg ccgggttatg gctgatcccg
     2521 ttgatgctgg gaatgaaaat catgcaggtc ggcacgcgag gcgcgaaaat ttgtgcgtcg
     2581 tgcccggcac cactgtgcat tacccggtaa ttcagttttt cgctttcaca caattctgtc
     2641 agggtggcga ccagctcctt attcatcggc accggttctt cgtccatcca taaatcgata
     2701 tcaataccaa tgtccatttc atcgcaaatc gcccgcatgt cgttttctaa ctgttgggtg
     2761 aaattgcgca gcacggcggc gtcggtatga cgacaatcaa tggtgaacgt ggttttcccc
     2821 ggcaccacat ttaccgtatt cgggcgcggt tccaccttgc caaaggtcag caccagcggg
     2881 tcgcccatct ttttcgcttt ttcgatcgac tgatggcaaa tgcgactgaa agcgtaaacc
     2941 gtatcacgac gataacccat cggcgtggtg cctgcatggt ttgattcgcc attcagcgtc
     3001 accgtatagc gacgttgccc gacaattgca ttcaccacgc caattgattg cccattactt
     3061 tccagcacac agccctgttc gatatgcagt tcgacaaagg ctttaatatc ctgacgcgga
     3121 gttagtgggg cgttcggcag agtaaatccg caagccttca tcgcatcgac aaaactattt
     3181 cctttggcat cacagatatt ccgcacgtcg tcaggattcg ccagcccaaa gatattttta
     3241 ctgccccaga agacatacgg gaagcggctg ccttcttctt ctgccatcgc caccacttcg
     3301 accgtacgta acggcgcgcc gtattgcgtt ttcaaccagt caattgccag ccacgccgcc
     3361 agtgcgccga attgtccgtc gaggttaccg ccgttaacca cggtatcgat atgcgaaccg
     3421 ctcagaacca cttcctgtgg atattcggtg ccactcaggc gaccgtataa attccccact
     3481 tcatcgaaac gtgtttccag cccgcttgct gccattcttt ttttaaattg ctgctgggtt
     3541 tccaaccatt ccggcgaata aagtaaacgg gtcattccac ccgttgggtc agagccaaaa
     3601 gaggaaagcc agggcagcgt ttcttctata gcttgacgga aatgtgtaat cataagaaag
     3661 tcctgtctca ataattattg cgcaaaggga tttttcgttt cgtatgacgt gttataaagc
     3721 gcgtcggaaa tcaaatactg gtaaatatca tcaacaattt cgatgccttc gacggcggct
     3781 ttgcgttgtt taatatcctg atcctgtccg ggataataaa cctgattaaa accgggcgcg
     3841 ggggtaatgg catttaattc gcgcatggtc tggctaagat gttgacggaa taattcgctg
     3901 gaggagaaaa agttcgggtt aataacgata tgtaattgcc ccaaattacg cccggcgtgt
     3961 aaatcgtcat acatcgaact aacctgtcgc ccaaacggta agccgagtaa gacgcctgag
     4021 aggacgtcaa tcatcatcat caggccatac cctttcggcc cagcggcggg gagcagagca
     4081 tgtaccgcga acggatcggt tgttggtgca ccgtttttat cgaccgccca ggtatccggg
     4141 atagacatat tacgcgaacg ggcgtcgagc acttttcccc atgcctgtac ggtagtcgcc
     4201 atatcaaagg taaggatctc gtcgccttct cccggcgcgg caaaggccag tgggttagta
     4261 ccgtagtaaa tttccgcgcc gccaaacggc accaccattg gatcggactg gcacatcgaa
     4321 atgccaatta acccggcgcg agctgcctgc tgtacaaaat aagagattgc gccgctgtga
     4381 cccatccggc tgataccgac caccgcaacg ccattttgct gggcggtttt gatggcatgt
     4441 tccataccca ttttcgccgc gacctgtccg gcggcattgt cggcatgtaa aattgccgag
     4501 cacggtccgg tttcttcaag acgaaattcc ggttcacggt tggtgccgcc ttttgaaatg
     4561 cgttcggcgt agtattccac gcgcaccgcg ccatgagagt ggatccctct ggcatcggcg
     4621 taaaccaata cttcagccac ggttgcagcg tgctcacgtt ttaacccagc ctggcagagt
     4681 ttattctcaa ttagctggtg gagtgtttcc cgactgattt tcatctgtct tcctttttaa
     4741 cgacgatgtg aagcatgact gcaattaaca tacagggaaa atatctggat tatgtgatcc
     4801 agacaggcaa aaaaatatag ttagaattta tttgataatc cgctcgcttt taacctggtt
     4861 tttaaaacaa tcacgcttat taaaaaataa cgcgtaatag cccggcggtt atttgaattc
     4921 ttttgttaat aatccctgtg tgatattcat caccttattt actcgttgtc atcgataccg
     4981 taatcgccac attaacactg ctcgtgcaat tgccatgggt gcaattttta aggagttgtt
     5041 atgatccacg cctttattaa aaaagggtgt tttcaggatt cggtcagttt aatgattatt
     5101 tcacgaaaac tcagcgaatc agaaaatgtt gatgatgttt ccgtaatgat gggtacgccc
     5161 gccaataaag cgttattaga taccacaggt ttctggcatg acgattttaa tcacgccacg
     5221 ccgaacgata tttgcgtggc aattcgtagc gaagcggcag atgcggggat cgcgcaggcg
     5281 gttatgcagc agcttgaaga ggcgctaaaa caactggcgc agggttcagg cagcagccag
     5341 gcgttgacgc aggtgcgtcg ttgggacagt gcctgtcaga aattacccga tgccagtctg
     5401 gcgctgattt cagtggctgg cgagtatgcg gcggagctgg cgaaccaggc gctggatcgc
     5461 aacctcaacg tgatgatgtt ctccgataac gtcacgctgg aagatgaaat ccaacttaaa
     5521 acccgcgcgc gggaaaaagg cttgctggtg atggggccgg actgcggtac gtcgatgatt
     5581 gccgccacgc cgctggcttt tgctaacgtg atgccggaag gcaacattgg cgtcattggc
     5641 gcttccggta ccgggattca ggagctgtgc tcacagattg cgctggcagg ggagggaatt
     5701 actcatgcga ttggccttgg cgggcgcgac ctcagccgtg aagtgggcgg catcagtgcg
     5761 ctaacggcgc tggaaatgct tagtgcagac gagaaaagcg aagtgctggc atttgtttca
     5821 aaaccacctg ccgaagctgt gcgtctgaaa attgttaatg ccatgaaagc aactggcaaa
     5881 ccgacggtgg cgttgttttt aggttatacc ccggcggtgg cccgcgacga gaatgtctgg
     5941 tttgcctcct cgctggatga ggccgcacgc ctggcttgtc tgctttcacg cgtcactgcg
     6001 cgacgtaacg cgatagcgcc tgtcagcagc ggatttattt gcggtttgta taccggcggt
     6061 acgctggctg ctgaagcggc gggattactt gccggacacc ttggcgtgga agccgacgat
     6121 acccatcaac atggcatgat gctggacgcc gacggacatc agatcctcga cctgggcgat
     6181 gatttttaca ccgtcgggcg tccccatccg atgatcgacc cgaccttacg caacctgtta
     6241 attgccgatc tcggcgctaa accgcaagtg cgcgtgttgc tgcttgatgt cgtgattggc
     6301 ttcggtgcga ccgccgatcc tgccgcctcg ctggtgagcg cctggcaaaa agcctgtgcc
     6361 gcgcgttcag acaatcaacc actgtatgcc attgccacgg tgacaggcac tgaacgtgac
     6421 ccgcaatgcc gcgcgcagca aatcgccacg ttggaagatg cggggatcgc ggtagtgagt
     6481 tcgctaccgg aagccacctt gctggcggcg gcgttaattc atccgctctc gcctgccaca
     6541 cagcaacaca caccgtcatt actggaaaac gtcgccgtga ttaacatcgg attacgcagc
     6601 tttgcgctgg agctacaaag cgccagcaaa ccggttgtgc attaccaatg gtcgccagtc
     6661 gccggcggca ataaaaaact ggctcgttta ttagaacgtt tgcaataagg ggttcccatg
     6721 tttacatcag tggcgcaagc caatgctgcg gttatcgaac aaattcgtcg cgctcgtcca
     6781 cactggctgg atgtgcaacc ggcttcttca cttatcagcg aactaaacaa gggcaaaaca
     6841 ctgcttcacg ccgggccgcc aatgcgctgg caggagatga ccggacccat gaaaggggcg
     6901 tgcgtgggcg catgtctgtt cgaaggctgg gcgaaagatg aagcgcaggc gctggcgatg
     6961 ctggagcagg gggaagtgaa cttcattcct tgtcaccatg tgaatgccgt cgggccaatg
     7021 ggtggcatta cttctgccag tatgccgatg ctggtggtcg agaacgtgac cgacggcaac
     7081 cgggcctact gcaacctcaa cgaaggtatc ggcaaagtga tgcgttttgg cgcttacggc
     7141 gaagatgtcc tgactcgcca tcgctggatg cgcgatgtgt taatgccagt attaagcgcg
     7201 gcgctggggc gcatggagcg cggtatcgat ctcaccgcga tgatggcaca gggcattacc
     7261 atgggcgatg agttccatca gcgcaacatt gcatcctcag cactgttgat gcgcgcgctg
     7321 gccccacaaa ttgctcgcct caatcacgat aaacagcaca tcgcggaagt gatggatttc
     7381 ctcagcgtga ccgatcagtt cttcctcaac ctcgcgatgg cctattgcaa ggcggcgatg
     7441 gatgcaggcg cgatgatccg cgcgggcagc atcgttacgg ctatgacccg caacggcaat
     7501 atgttcggga ttcgggtaag tgggctgggc gagcgctggt ttactgcgcc tgtaaacact
     7561 ccgcaaggtc tgtttttcac cggcttctcg caggagcagg cgaacccgga tatgggcgat
     7621 agcgcgatta ccgaaacctt tggtatcggt ggtgcggcaa tgatcgccgc ccctggcgta
     7681 acacgctttg tcggtgcggg tggtatggaa gcagccagag cagtatctga agagatggca
     7741 gaaatcttcc ttgaacgcaa tatgcagttg cagatccctg gctgggattt ccagggcgcg
     7801 tgtctggggc tggatattcg tcgcgtggta gaaaccggca ttacaccact catcaatacg
     7861 ggtatcgccc ataaagaggc ggggatcggg cagattggtg caggcaccgt gcgggcaccg
     7921 ctggcgtgct ttgaacaggc gctggaagct ctggctgaaa gcatgggtat tggttgagga
     7981 acgcgcaatg acgatcatcc atcctctgct tgccagccgt agtgcaccga attattgcca
     8041 gtcctggcgg ttagcgggca tctggcggcg ggcgattaac ctgatgacgg aaagcggcga
     8101 actgttaacg ctgcatcgtc aggggagtgg tttcggcccc ggaggatggg tgcttcgccg
     8161 cgcgcaattc gatgcgttat gcggtggatt atgcggcaat gaacgaccac aggttgtggc
     8221 tcaaggcatt cgcctcggac gtttcacggt taaacagcca cagcgttatt gtttgctgcg
     8281 tattacgccg cctgcgcatc ctcaaccgct tgcagctgca tggatgcaac gcgcggagga
     8341 aaccgggctt ttcgggccac tggcgatggc ggcaagcgat ccgctgcctg ctgagttacg
     8401 ccagtttcgt cactgttttc aggccgcgct caatggcgtt aagaccgact ggcggcactg
     8461 gctgggtaaa ggcccaggat taacgccaag tcatgatgac acgctgagcg gaatgctgct
     8521 ggcggcctgg tattatggcg ctttagatgc gcgctccggt cgcccgtttt ttgcctgttc
     8581 cgacaatctt caactcgtta ccacagcggt gagcgtcagt tatttacgtt atgccgcgca
     8641 aggatatttc gcctcgccac tcctgcactt tgttcatgct ctgagttgcc cgaaacgtac
     8701 cgcagcggcg atcgattcgc tgctggcgct ggggcatacg tcaggggccg atacgctgct
     8761 ggggttctgg cttggccaac aattattaca aggaaaacca tgaaaacact ggttgtggct
     8821 cttgggggca acgccttact ccagcgcggt gaggcgctga cggcagaaaa tcaatatcgc
     8881 aatattgcca gtgctgtacc cgcgctggcg cgcctggccc gttcttatcg gctggcgatt
     8941 gttcacggca acgggccaca ggtggggctg ttggcgttac agaatctggc gtggaaagag
     9001 gtagaaccgt atccgctgga tgtgctggtg gcggaaagcc aggggatgat tggctatatg
     9061 ctggtgcaga gcttgagcgc acagccgcag atgccgcctg tcacgacggt gctgacgcgc
     9121 attgaggttt cgcctgatga tccggcgttt ttgcagccag agaagtttat cggtccggtt
     9181 tatcagccag aagaacaaga ggcactggaa gctgcttacg gctggcagat gaaacgtgat
     9241 ggtaaatatt tgcgccgggt ggtggcgtct ccgcaaccgc gtaatattct cgacagcgaa
     9301 gccatcgagt tgttgctcaa agaggggcat gtggtgattt gcagtggcgg cggcggtgtg
     9361 cctgtgacgg atgacggagt agggagtgaa gcagtgattg ataaagatct cgccgctgcg
     9421 ttgctcgccg agcagattaa tgccgatgga ctggtgatcc tcaccgatgc tgatgcggta
     9481 tatgaaaact ggggaacgcc gcagcaacgt gccattcgcc atgccacacc ggatgagtta
     9541 gcgccacttg ccaaagccga tggttcgatg gggccgaagg taacggcagt gagtggctat
     9601 gtcagaagcc gtggtaaacc cgcgtggatt ggggcgttat cgcgaattga agagacgctg
     9661 gcgggcgaag cggggacctg tatttcgctg tagtgatggg caccaggcaa ttggcacatc
     9721 tagcctgatg cgatgctggc gcgtcttatc aggtctacag gcggtgtcgg aacggtaggc
     9781 cggataaggc gttcacgccg catccggcaa tcggcacacc atgcctgatg cgatgctggc
     9841 gcatcttatc aggcctacag tcggtgttgg aacggtaggc cggataaggc gttcacgccg
     9901 catgcggcaa gaaaatgagc atcaattaac tgaacttgct ctgcgcccaa atcacgccgc
     9961 tagcatattc tggcggcagc agcgggatta aggcttccag cgtcgcggtc agacgcgatg
    10021 tgtcgctgtc ggtcaaattt aaatgcccca ctttacgccc cggacggact tctttgtcgt
    10081 accagtgcag atgcaccagc ggcagtttca gccagtcata attcacatcg ctaccaatca
    10141 ggttgatcat caccgacgga ctattcacca ccggttgtgg taacggcaga tcggtaatcg
    10201 cccgcagatg cagctcaaac tggctgatgc tggcaccgtt ttgcgtccag tgcccactgt
    10261 tatgcacacg cggagccagt tcgttgatca gcagaccttg tggggtgaca aaacactcca
    10321 tcgccatcac gcccacatag cccagctcct gcataatcgc cgacagcatc tcttcggcct
    10381 gcgcctgctg ctgcgcgttg gcctgcggga aagcgacgct ggtgcgcaaa ataccgtcct
    10441 gatgcaggtt atgcgtcagc ggataaaaca cggtactacc atcaaagccg cgcgcgccaa
    10501 ccagcgacac ttcaccagag aagtttatgc cctgctcgac aatacattcg ccgtagcatt
    10561 cagtcggtaa ctgttcggtt tcatcggcgc gtaaacgcca ttgaccgcgt ccgtcatagc
    10621 caccagtgcg acgcttaaca atcgccagct cacctaaacg atcaaacacc gcaggccact
    10681 cgctgcgatc ggcaagtaac tgccacggtg cggtcggcag gtggagctta tcgaaaagct
    10741 gcttctgagt cagacggtca gcaataatcg ggaacacatc gcggttcaca aaggccggat
    10801 ggcgcgccag ctcgcgggtt aatgcggttt ccggccagcg ttcgatctca gcggtaatca
    10861 cactttgttg aaaaggcacc gccgccggtt cagcgtccag cccgactggc cagacagcaa
    10921 tgcctaacgg ttcgcctgcc tgacgcagca tacggcctaa ctgcccgtta ccgaggacgc
    10981 aaacctgttt catgccgcac ctcgcgggtc cgggttttcc agcacttcgt cagtctgggc
    11041 tttgcgccag tcattcagac gctggtgcag ttctttatca tgagtcgcaa gaatttgtgc
    11101 tgccagtaac gccgcgtttg ccgcgccagc tttaccaatc gccagcgtac ccaccggaat
    11161 gccgcgcggc atttgtacga tggagtagag actatcgaca ccgctcagtg cggcgctctg
    11221 tactggcacg cccagcaccg gcaccagcgt tttggcggca atcatacctg gcaggtgcgc
    11281 cgcgccgcct gcgcccgcaa taatcacctg ataaccgttt tcttcggcgc tttcggcgaa
    11341 gctgaacagt ttgtcggggg tgcggtgagc agaaaccact tcaacgtggt gcgggacatt
    11401 caggatttcg aagatttcgg cggcgaactg catggtagcc cagtcgcttt tggaccccat
    11461 cacgatggcg acacgcgccg gattattgcg ggaagacatg cgtcttaaaa ctcctgtggt
    11521 gcacaactct cggctttaga gggcacagag aatagcacgg aaagagagca aggaaaacgg
    11581 ttgcgtggct gtgaaatcag caaagttgcg ggtatttaaa acggaaaatg aatcagctca
    11641 acgtcatccg ccgtgacttt caccattgaa ccttccgtat gccaggcacc cagcacgacg
    11701 cggaaagcag gttgctgatt ggcgataagt tcatgtactg ccgggcgatg ggtatgcccg
    11761 tgaatcagcc attgcacctg atgtttttcc atcgcactga ccaccgcgtt ttggttaacg
    11821 tccatgatcg ccagcgattt actgctgttg gcttctttgc tgttcgctcg cattcgcgcg
    11881 gcaatgcgtt tgcgcacaaa caacggcagg gcgaggaata gcgtttgcag ccacggcttg
    11941 tggactttgg cgcgaaaagc ctgataaccg gcgtcatcgg tgcacagcgt gtcgccatgc
    12001 ataatcaaca cccgacgacc ataaagttcg agcacctttt cttccggcaa taacgtcatg
    12061 ccgctttcac gggcaaagcg tttgccgagc agaaaatcac ggttgccatg aatgaaataa
    12121 cagggaacgc cggaatcgga caccgctttg atcgctgccg ccatctggcg atggagtggg
    12181 ttgggatcgt cgtcgccaat ccacgcttca aacagatcgc caagaatata cagcgcgtcg
    12241 gccttgcggg cttcccccgc taaaaaacgc agaaaaccgg cggtgatcgc cggttcttcc
    12301 acgcagagat gaagatctgc aataaagagt gtcgccacga ttactcgcta acggtcacgc
    12361 tttcaatgat aacgtcttct tttggcacgt cctggtgcat accgctacga ccggttgcaa
    12421 cacctttgat tttgtctacc acgtccatgc cttcaaccac ttcagcaaac acgcagtaac
    12481 cccaaccttg caggctttcg ccagagaagt tcaggaagtc gttatcaact acgttgatga
    12541 agaactgtgc agttgcagag tgcggtgcct gagtacgggc cattgccagc gtaccacggg
    12601 tatttttcag accgttattg gcttcgtttt tgatcgggtc tttggtggtt ttttgcttca
    12661 tgccaggttc aaaaccgccg ccctgaatca taaagccgtt gattacacgg tggaaaatgg
    12721 tgttgttgta aaaaccttcg cggcagtagt ccaggaagtt tttaactgtt tcaggtgctt
    12781 tatcgtcaaa agttttgatg acaatatcgc cgtgattggt gtggaaagta accatttttg
    12841 catcctgttc cgtttgattg gtgcttcaac ccggttcggg tcatatatag gggggtgtta
    12901 tagcataacc gcacgatcgg atcatcacgc aatgtatgct gattcgcgcg ggaaatatgg
    12961 gtattatacg caactcaatt acccacacat gtctaaacgg aatcttcgat gctaaaaatc
    13021 ttcaatactc tgacacgcca aaaagaggaa tttaagccta ttcacgccgg ggaagtcggc
    13081 atgtacgtgt gtggaatcac cgtttacgat ctctgtcata tcggtcacgg gcgtaccttt
    13141 gttgcctttg acgtggtcgc gcgctacctg cgtttcctcg gctataagct gaagtatgtg
    13201 cgcaacatta ctgatatcga cgacaaaatc atcaaacgcg ccaatgaaaa tggcgaaagc
    13261 tttgtggcgc tggtggatcg catgatcgcc gaaatgcaca aagattttga tgctttgaac
    13321 attttgcgcc cggatatgga gccgcgcgcg acgcaccata tcgcagaaat tattgaactc
    13381 actgaacaac tgatcgccaa aggtcacgct tatgtggcgg acaacggcga cgtgatgttc
    13441 gacgtcccga ccgatccaac ttatggcgtg ctgtcgcgtc aggatctcga ccagctgcag
    13501 gcaggcgcgc gcgttgacgt ggtcgacgac aaacgcaacc cgatggactt cgttctgtgg
    13561 aagatgtcga aagagggcga accgagctgg ccgtctccgt ggggcgcggg ccgtcctggc
    13621 tggcacattg agtgttcggc aatgaactgc aagcagctgg gtaaccactt tgatatccac
    13681 ggcggcggtt ctgacctgat gttcccgcac cacgagaacg aaattgccca gtccacctgt
    13741 gcgcacgatg gccagtatgt gaattactgg atgcactccg gtatggtgat ggttgaccgc
    13801 gagaagatgt ccaaatcgct gggtaacttc ttcaccgtgc gtgacgtgct gaaatactac
    13861 gatgcggaaa ctgtgcgtta cttcctgatg tcgggtcact atcgcagtca gttgaactac
    13921 agtgaagaga acctgaaaca ggcgcgtgcg gcgctggaac gtctctacac tgcgctgcgc
    13981 ggcacagaca aaaccgttgc gcctgccggt ggcgaagcgt ttgaagcgcg ctttatcgag
    14041 gcgatgaacg acgatttcaa caccccggaa gcctattccg tactgtttga tatggcgcgt
    14101 gaagtaaacc gtctgaaagc agaagatatg gcagcggcga atgcaatggc gtctcacctg
    14161 cgtaaacttt ctgccgtatt gggcctgctg gagcaagaac cggaagcgtt cctgcaaagc
    14221 ggtgcgcagg cagacgacag cgaagtggct gagattgaag cgttaattca acagcgtctg
    14281 gatgcccgta aagcgaaaga ctgggcggcg gcagatgcgg cgcgtgaccg tcttaacgag
    14341 atggggatcg tgctggaaga tggcccgcaa gggaccacct ggcgtcgtaa gtaattgcgc
    14401 tattgccgga tgcgagtttt cgcatccggt tatcgtctgc gccaccacaa cattcccatc
    14461 agtagcatcc ccggcaacca cacccacatc aattcagaaa taatcacctg atgcccgtac
    14521 ggcgtggtgt aacgagacaa tgcaaacggc gcgactttaa tcacctgcca gggagcgaaa
    14581 aagagttcat ctgaccacgg ccacagccag ccaacccctt taccgccagt ggttaccgaa
    14641 tccagcaagc tgtgcgatag caacgagacg gttaaaaaca gccagcagcg aatcagccca
    14701 gccctgaacc atcgacgtcc aatcagcaca cataacagcg ggacaacaaa cgcaaacacc
    14761 agcgaatggg taaacccgcg atgaccaaaa atgttgccgt aagcaacgcc aaatttaaac
    14821 gataatacgt cggcatcagg caacatcgcc aggacgattc cggcaaataa cagacgcggt
    14881 gggatggctt tcgaacccaa ccctaaacca atgcataggg gaacggcggc gtgcgtaata
    14941 acggttggca tgatgatcgc ttcggcaaaa cgtcgatgct atcagcatgg atgaacgggg
    15001 cgtagagggc aaaagtctga aaagagaacc ggcctgttga tacaggccgg gaaagggatc
    15061 aggcaacaac ctgtacgctg tgacctgcaa aactcactgt ctgcccggcg acgattttgc
    15121 agcgtttgcg cgtttcaacc gcaccgtcga ctttcacctg gccttcggca atcgcgattt
    15181 tcgcctgtgc gccgctttcg ctccagcctt ccagtttcag caagtcgcac agctcaacgt
    15241 gcggatgttt acctaaagaa aatgtcgcca tgttactcac cctgtggatc atgatattca
    15301 acgcacgcct gtagcgtgtt ttcaatcagc gtggcaaccg tcatcgggcc aacgccgccg
    15361 ggaacaggcg taatgtatga ggcgcgttta gccgcgtctt caaacaccac gtcgcccaca
    15421 actttgccat tttccagacg gttgatgccg acatcaatca caattgcgcc ttctttgatc
    15481 cagtcaccag gaataaagcc cggcttgccg acggcaacga tcagtagatc ggcattttct
    15541 acgtggtgac gcagattttt agtgaaacgg tgagtcacgg tggtggtgca acctgccagt
    15601 agcagttcca tgctcatcgg gcggccaacg atattcgatg cgccaatcac cacggcgttg
    15661 aggccgaagg tatcaatgtt gtaacgctca agcagcgtga caataccgcg cggggtgcag
    15721 ggacgcagac gcggcgcgcg ctggcacagg cgaccgacgt tataaggatg gaaaccgtct
    15781 acgtctttgt ccgggtgtat acgttccagc actttgacgt tatcaatacc cgccggtaac
    15841 ggcagttgaa ccagaatgcc atcgatggtg ttgtcggcat tcagcgtatc gataagctcc
    15901 agcagctccg cttcgctggt ggtttctggg aggtcgtaag agcgggagac gaacccgact
    15961 tcttcacaag ccttgcgttt gcttgcgaca taaatttgcg atgcagggtt actacccacc
    16021 agcacaacgg ccagtcctgg tgcccgcagt ccggctgcaa tacgcgcctg aactttttga
    16081 gcaacttcag agcgcacctg ctgcgcaatc gttttaccgt caataatctt tgctgccatc
    16141 agagagagga ttccatctgt tacgtaggtc gaaggggatg cgcctatttt gtcagaagcg
    16201 gggcgcgctg tcaggtttcg tttcagattt atcgcgtgaa gcgacctctt gcgaaggtga
    16261 ggcgcaccgt cgctgagatt gaaagcttca tttttcgtcc atgatgacgt tgtaaatctg
    16321 gaactgattt atttccttgt ctaaggatta agataattta agaaatacct gacaatataa
    16381 aaagaatttt cagcctggta atttaccgct tcaggtctat atttgtgttg aatatatttt
    16441 gcgcggaaat attcatctaa cggggctctc tattttttag aatagagtgc atattttcaa
    16501 ttaagacatt cttagaggat aaaaaggaat tttactacta tcagtgtctt aaataaagta
    16561 atcggttata tacggatgtg gagtcgataa atgagattga aggaatatat atgaaattaa
    16621 gatttatttc gtctgcgctg gctgccgcac tattcgccgc tacgggtagt tatgctgccg
    16681 ttgtagatgg cggtacaatt cactttgaag gcgaactggt gaatgctgcc tgttcagtga
    16741 atactgactc ggcagaccag gttgtcacac tcggtcaata tcgtaccgat attttcaatg
    16801 ctgttggtaa tacctctgca ttaattccat tcactataca gttgaacgac tgcgatcctg
    16861 tcgttgccgc taatgctgcc gttgcatttt ctggtcaggc ggatgcaatc aatgataatt
    16921 tattggccat tgcatccagt actaatacaa caacagcaac gggtgtcggt attgaaatac
    16981 ttgataatac atccgcaatt ctcaaacctg atgggaatag cttctcaacc aaccagaact
    17041 tgatccccgg gaccaacgtt cttcattttt ctgcacgtta taaaggcacc ggtacaagtg
    17101 catcagcagg gcaagcaaat gctgacgcga cttttattat gagatacgaa taatcaaaac
    17161 cacgttgttt tgaattatat atcacgtctt ataacaaagt aatgtaccgg ttgtctgaag
    17221 cggtatggtg gcaatgtaaa tcgaaatcat gttcactttg tatcatgccg ctttattaaa
    17281 tgaaaaggga atgatgtgtg taagaaacca aagcaatcat ttctttatac tccttatttt
    17341 tgccgtcagg aatacacaag gcgtattaac tatgatgact aaaataaagt tattgatgct
    17401 cattatattt tatttaatca tttcgaccag cgcccatgct gccggaggga tcgcattagg
    17461 tgccacgcgt attatttatc ccgctgatgc taaacagact gcggtatgga tcagaaatag
    17521 ccataccaat gagcgctttc tggtcaattc gtggattgaa aacagcagcg gtgtaaaaga
    17581 aaagtcattc atcattac
//
LOCUS       DAMZFT010000102        17475 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.102, whole genome shotgun sequence.
ACCESSION   DAMZFT010000102 DAMZFT010000000
VERSION     DAMZFT010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17475)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17475)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17475
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.102"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..300
                     /gene="ydcO"
                     /locus_tag="PN977_003561"
     CDS             <1..300
                     /gene="ydcO"
                     /locus_tag="PN977_003561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BenE family transporter YdcO"
                     /protein_id="HDI6043562.1"
                     /translation="AVAGIFYLLAGLFGSAITGMMTTLPVSWIQMLAGLALLSTIGGS
                     LYQALHNERERDAAVVAFLVTASGLTLVGIGSAFWGLIAGGVCYVVLNLIADRNR"
     gene            complement(335..1003)
                     /locus_tag="PN977_003562"
     CDS             complement(335..1003)
                     /locus_tag="PN977_003562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3313 domain-containing protein"
                     /protein_id="HDI6043563.1"
                     /translation="MRTTSFAKVAALCGLLALSGCASKITQPDKYSGFLNNYSDLKET
                     TSATGKPVLRWVDPSFDQSKYDSIVWNPITYYPVPKPSTQVGQKVLDKILNYTNTEMK
                     EAIAQRKPLVTTAGPRSLIFRGAITGVDTSKEGLQFYEVVPVALVVAGTQMATGHRTM
                     DTRLYFEGELIDAATNKPVIKVVRQGEGKDLNNESTPMAFENIKQVIDDMATDATMFD
                     VNKK"
     gene            complement(1306..1899)
                     /gene="tehB"
                     /locus_tag="PN977_003563"
     CDS             complement(1306..1899)
                     /gene="tehB"
                     /locus_tag="PN977_003563"
                     /EC_number="2.1.1.265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance methyltransferase TehB"
                     /protein_id="HDI6043564.1"
                     /translation="MIIRDENYFTDKYELTRTHSEVLEAVKVVKPGKTLDLGCGNGRN
                     SLYLAANGYDVDAWDKNAMSIANVERIKSIENLDNLHTRVVDLNNLTFDGQYDFILST
                     VVLMFLEAKTIPGLIANMQRCTKPGGYNLIVAAMDTADYPCTVGFPFAFKEGELRRYY
                     EGWERVKYNEDVGELHRTDANGNRIKLRFATMLARKK"
     gene            complement(1896..2888)
                     /gene="tehA"
                     /locus_tag="PN977_003564"
     CDS             complement(1896..2888)
                     /gene="tehA"
                     /locus_tag="PN977_003564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate transporter/tellurite-resistance
                     protein TehA"
                     /protein_id="HDI6043565.1"
                     /translation="MQSDKVLNLPAGYFGIVLGTIGMGFAWRYASQVWQVSHWLGDGL
                     VILAMIIWGLLTGAFITRLIRFPHSVLAEVRHPVLSSFVSLFPATTMLVAIGFVPWFR
                     PLAVCLFSFGVVVQLAYAAWQTAGLWRGSHPEEATTPGLYLPTVANNFISAMACGALG
                     YTDAGLVFLGAGVFSWLSLEPVILQRLRSSGELPTALRTSLGIQLAPALVACSAWLSV
                     NGGEGDTLAKMLFGYGLLQLLFMLRLMPWYLSQPFNASFWSFSFGVSALATTGLHLGS
                     GSDNGFFHTLAVPLFIFTNFIIAILLIRTFALLMQGKLLVRTERAVLMKAEDKE"
     gene            3012..3992
                     /locus_tag="PN977_003565"
     CDS             3012..3992
                     /locus_tag="PN977_003565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6043566.1"
                     /translation="MRKYRLSEEQRAFSYQEDGTKKNVLLRQIIAISDFNDVIAGTAG
                     GWIDRETVLAQEGNCWIYDQNAIAFGGTVISGNTRITGTSVLWGEVYATDNVWIDNSE
                     ISQGAYISDSVTISDSLVCGQCRIFGHALIDQHSMIVAAQGLTPDHQLLLQIYDRARV
                     SASRIVHQAQIYGDAVVRYAFIEHRAEVFDFASIEGNEENNVWLCDCAKVYGHAQVKA
                     GIEEDAIPTIHYSSQVAEYAIVEGNCVLKHHVLIGGNAVVRGGPILLDEHVVIQGESR
                     ITGAVIIENHVELTDHAVVEAFDGDTVHVRGPQVINGEERITRTPLAGLL"
     gene            complement(3984..4523)
                     /gene="rimL"
                     /locus_tag="PN977_003566"
     CDS             complement(3984..4523)
                     /gene="rimL"
                     /locus_tag="PN977_003566"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12-serine
                     acetyltransferase"
                     /protein_id="HDI6043567.1"
                     /translation="MTETIKVSESLELHAVAESHVTPLYQLICKNRTWLQQSLNWPQF
                     VQSEEDTRKTVQGNVMLHQRGYAKMFMIFKEDELIGVISFNRIEPLNKTAEIGYWLDE
                     SHQGQGIISQALQALIHHYAQSGELRRFVIKCRVDNPQSNQVALRNGFILEGCLKQAE
                     FLNDAYDDVNLYARIIDSQ"
     gene            complement(4586..4810)
                     /locus_tag="PN977_003567"
     CDS             complement(4586..4810)
                     /locus_tag="PN977_003567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415943.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcH family protein"
                     /protein_id="HDI6043568.1"
                     /translation="MFPEYRDLISRLKNENPRFMSLFDKHNKLDHEIARKEGSDGRGY
                     NAEVVRMKKQKLQLKDEMLKILQQESVKEV"
     gene            complement(4950..6605)
                     /gene="opgD"
                     /locus_tag="PN977_003568"
                     /gene_synonym="mdoD"
     CDS             complement(4950..6605)
                     /gene="opgD"
                     /locus_tag="PN977_003568"
                     /gene_synonym="mdoD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucan biosynthesis protein OpgD"
                     /protein_id="HDI6043569.1"
                     /translation="MDRRRFIKGSMAMAAVCGTSGIASLFSQAAFAADSDIADGQTQR
                     FDFSILQSMAHDLAQTAWRGAPRPLPDTLATMTPQAYNSIQYDAEKSLWHNVENRQLD
                     AQFFHMGMGFRRRVRMFSVDPATHLAREIHFRPELFKYNDAGVDTKQLEGQSDLGFAG
                     FRVFKAPELARRDVVSFLGASYFRAVDDTYQYGLSARGLAIDTYTDSKEEFPDFTAFW
                     FDTVKPGATTFTVYALLDSASITGAYKFTIHCEKSQVIMDVENHLYARKDIKQLGIAP
                     MTSMFSCGTNERRMCDTIHPQIHDSDRLSMWRGNGEWICRPLNNPQKLQFNAYTDNNP
                     KGFGLLQLDRDFSHYQDIMGWYNKRPSLWVEPRNKWGKGTIGLMEIPTTGETLDNIVC
                     FWQPEKAVKAGDEFAFQYRLYWSAQPPVHCPLARVMATRTGMGGFPEGWAPGEHYPEK
                     WARRFAVDFVGGDLKAAAPKGIEPVITLSSGEAKQIEILYIEPIDGYRIQFDWYPTSD
                     STDPVDMRMYLRCQGDAISETWLYQYFPPAPDKRQYVDDRVMS"
     gene            complement(6830..8173)
                     /locus_tag="PN977_003569"
     CDS             complement(6830..8173)
                     /locus_tag="PN977_003569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDI6043570.1"
                     /translation="MANSITADEIREQFSQAMSAMYQQEVPQYGTLLELVADVNLAVL
                     ENNPQLHEKMVNADELARLNVERHGAIRVGTAQELATLRRMFAIMGMYPVSYYDLSQA
                     GVPVHSTAFRPIDDASLARNPFRVFTSLLRLELIENEFLRQKAAEILRQRDIFTPRCR
                     QLLEEYEQRGGFNETQAQEFVQEALETFRWHQSATVDEETYRALHNEHRLIADVVCFP
                     GCHINHLTPRTLDIDRVQSMMPECGIEPKILIEGPPRREVSILLRQTSFKALEETVLF
                     AGQKQGTHTARFGEIEQRGVALTPKGRQLYDDLLRNAGTGQDNLTHQMHLQETFRTFP
                     DSEFLMRQQGLAWFRYRLTPSGEAHRQAIHPGDDPQPLIERGWVVAQPITYEDFLPVS
                     AAGIFQSNLGNETQARSHGNASREAFEQALGCPVLDEFQLYQEAEERSKRRCGLL"
     gene            8390..9313
                     /locus_tag="PN977_003570"
     CDS             8390..9313
                     /locus_tag="PN977_003570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDI6043571.1"
                     /translation="MEKNSLFSQRIRLRHLHTFVAVAQQGTLGRAAETLNLSQPALSK
                     TLNELEQLTGARLFERGRQGAQLTLPGEQFLTHAVRVLDAINTAGQSLHRKEGLNNDI
                     VRVGALPTAALGILPSVIGQFHQQQKETTLQVATMSNPMILAGLKTGEIDIGIGRMSD
                     PELMTGLNYELLFLESLKLVVRPNHPLLQENVTLSRVLEWPVVVSPEGTAPRQHSDAL
                     VQSQGCKIPSGCIETLSASLSRQLTVEYDYVWFVPSGAVKDDLRHATLVALPVPGHGA
                     GEPIGILTRVDATFSSGCQLMINAIRKSMPF"
     gene            complement(9352..10992)
                     /gene="trg"
                     /locus_tag="PN977_003571"
     CDS             complement(9352..10992)
                     /gene="trg"
                     /locus_tag="PN977_003571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein Trg"
                     /protein_id="HDI6043572.1"
                     /translation="MNTTPSQRLGFLHHIRLVPLFACILGGILVLFALSSALAGYFLW
                     QADRDQRDVTAEIEIRTGLANSSDFLRSARINMIQAGAASRIAEMEAMKRNIAQAESE
                     IKQSQQGYRAYQNRPVKTPADEALDTELNQRFQAYITGMQPMLKYAKNGMFEAIINHE
                     SEQIRPLDNAYTDILNKAVKIRSTRANQLAELAHQRTRLGGMFMIGAFVLALVMTLIT
                     FMVLRRIVIRPLQNAAQRIEKIASGDLTMNDEPAGRNEIGRLSRHLQQMQHSLGMTVG
                     TVRQGAEEIYRGTSEISAGNADLSSRTEEQAAAIEQTAASMEQLTATVKQNADNAHHA
                     SKLAQEASIKASDGGQMVSGVVKTMGAISTSSKKISEITAVINSIAFQTNILALNAAV
                     EAARAGEQGRGFAVVASEVRTLASRSAQAAKEIEGLISESVRLIDLGSDEVATAGKTM
                     STIVDAVASVTHIMQEIAAASDEQSRGITQVSQAISEMDKVTQQNASLVEEASAAAVS
                     LEEQAARLTEAVDVFRLNKHSVSAEPRGAGEPVSFATV"
     gene            11391..11540
                     /gene="hokB"
                     /locus_tag="PN977_003572"
     CDS             11391..11540
                     /gene="hokB"
                     /locus_tag="PN977_003572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokB"
                     /protein_id="HDI6043573.1"
                     /translation="MKHNPLVVCLLIICITILTFTLLTRQTLYELRFRDGDKEVAALM
                     ACTSR"
     gene            complement(11612..11785)
                     /locus_tag="PN977_003573"
     CDS             complement(11612..11785)
                     /locus_tag="PN977_003573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6043574.1"
                     /translation="MKKLALILVIGALVSFYADAGRKPCSGSKGGISHCTAGGKFVCN
                     DGSISASKKTCTN"
     gene            complement(12030..12560)
                     /gene="cybB"
                     /locus_tag="PN977_003574"
     CDS             complement(12030..12560)
                     /gene="cybB"
                     /locus_tag="PN977_003574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b561"
                     /protein_id="HDI6043575.1"
                     /translation="MESKYSRLQIGIHWLVFLLVIAAYCAMEFRGFFPRGDRPLINMI
                     HVSCGISILVLMVVRLLLRLKYPTPPIVPKPKPMMTGLAHLGHLVIYLLFIALPVIGL
                     VMMYNRGNPWSAFGMTMPYAAEANFERVDSLKWWHETLANLGYFVIGLHAAAALAHHY
                     FWKDNTLLRMMPRKRS"
     gene            12749..13750
                     /gene="gap"
                     /locus_tag="PN977_003575"
     CDS             12749..13750
                     /gene="gap"
                     /locus_tag="PN977_003575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HDI6043576.1"
                     /translation="MSKVGINGFGRIGRLVLRRLLEVKSNIDIVAINDLTSPKILAYL
                     LKHDSNYGPFPWSVDFTEDSLIVDGKSIAVYAEKEAKNIPWKAKGAEIIVECTGFYTS
                     AEKSQAHLDAGAKKVLISAPAGEMKTIVYNVNDDTLDGNDTIVSVASCTTNCLAPMAK
                     ALHDSFGIEVGTMTTIHAYTGTQSLVDGPRGKDLRASRAAAENIIPHTTGAAKAIGLV
                     IPELSGKLKGHAQRVPVKTGSVTELVSILGKKVTAEEVNNALKQATTNNESFGYTDEE
                     IVSSDIIGSHFGSVFDATQTEITAVGDLQLVKTVAWYDNEYGFVTQLIRTLEKFAKL"
     gene            complement(13791..15230)
                     /gene="aldA"
                     /locus_tag="PN977_003576"
     CDS             complement(13791..15230)
                     /gene="aldA"
                     /locus_tag="PN977_003576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HDI6043577.1"
                     /translation="MSVPVQHPMYIDGQFVTWRGDAWIDVVNPATEAVISRIPDGQAE
                     DVRKAIDAAERAQPEWEALPAIERASWLRKISAGIRKRASEISALIVEEGGKIQQLAE
                     VEVAFTADYIDYMAEWARRYEGEIIQSDRPGENILLFKRALGVTTGILPWNFPFFLIA
                     RKMAPALLTGNTIVIKPSEFTPNNAIAFAKIVDEIGLPRGVFNLVLGRGETVGQELAG
                     NPKVAMVSMTGSVSAGEKIMATAAKNITKVCLELGGKAPAIVMDDADLELAVKAIVDS
                     RVINSGQVCNCAERVYVQKGIYDQFVNRLGEALQAVQFGNPAERNDIAMGPLINAAAL
                     ERVEQKVARAVEEGARVALGGKAVEGKGYYYPPTLLLDVRQEMSIMHEETFGPVLPVV
                     AFDTLEEAISMANDSDYGLTSSIYTQNLNVAMKAINGLKFGETYINRENFEAMQGFHA
                     GWRKSGIGGADGKHGLHEYLQTQVVYLQS"
     gene            complement(15427..16227)
                     /locus_tag="PN977_003577"
     CDS             complement(15427..16227)
                     /locus_tag="PN977_003577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HDI6043578.1"
                     /translation="MNITPFPTLSPATIDAINVIGQWLAQDDFSGEVPYQADCVILAG
                     NAVMPTIDAACKIARDQQIPLLISGGIGHSTTFLYSAIAQHPHYNTIRTTGRAEATIL
                     ADIAHQFWHIPHEKIWIEDQSTNCGENARFSIALLNQAVERVHTAIVVQDPTMQRRTM
                     ATFRRMTGDNPDAPRWLSYPGFVPQLGNNADSVIFINQLQGLWPVERYLSLLTGELPR
                     LHDDSDGYGPRGRDFIVHVDFPAEVIQAWQTLKHDAVLIEAMESRSLR"
     gene            complement(16500..>17475)
                     /locus_tag="PN977_003578"
     CDS             complement(16500..>17475)
                     /locus_tag="PN977_003578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415931.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3418 domain-containing protein"
                     /protein_id="HDI6043579.1"
                     /translation="DDKNKKLKEGRSLQDLKDALKGKVQETLSAVADDGIEQSGLHIW
                     SFGQLPESYEQKRGNYKVKAWPALVDERDSVAIKLFDNPLEQKQAMWNGLRRLLLLNI
                     PSPIKYLHEKLPNKAKLGLYFNPYGKVLELIDDCISCGVDQLIDANGGPVWTEEGFAA
                     LHEKVRAELNDTVVDIAKQVEQILTAVFNINKRLKGRVDMTMALGLSDIKAQMGGLVY
                     RGFVTGNGFKRLGDTLRYLQAIEKRLEKLAVDPHRDRAQMLKVENVQQAWQQWINKLP
                     PARREDEDVKEIRWMIEELRVSYFAQQLGTPYPISDKRILQAMEQISG"
BASE COUNT         4474 a         4444 c         4056 g         4501 t
ORIGIN      
        1 gccgttgcag gcattttcta tttgctcgca ggtctgtttg gtagtgccat taccgggatg
       61 atgactaccc tgcccgtaag ttggatccag atgctggcag gtctggcgct gttgagtacc
      121 attggcggca gtttgtatca ggcgctgcat aatgagcgtg agcgcgacgc ggcggtggtg
      181 gcatttctgg taacggcaag tggattgacg ctggtcggga ttggttctgc gttttgggga
      241 ttaattgccg gaggcgtttg ttacgtggtg ttgaatttaa tcgctgacag aaaccgataa
      301 taaaaagcgc catctaacga tggcgcgtct ggaactactt tttgttaacg tcaaacatgg
      361 tggcatcggt cgccatgtca tcaataactt gtttaatatt ttcgaaagcc attggcgtac
      421 tttcgttatt caggtcttta ccttcgccct gacgaacgac tttgataacc ggtttattag
      481 tcgctgcatc aatcagctca ccttcaaaat acaagcgagt atccatggta cggtggcctg
      541 tagccatttg cgtccccgca accactaatg caacaggaac cacttcatag aattgcagcc
      601 cttctttgct ggtatctaca ccggtaatgg ccccacggaa aatcagacta cgcggcccag
      661 cggtggtaac cagtggttta cgctgcgcta tcgcttcttt catttcggtg ttggtatagt
      721 tcaaaatttt atccagaact ttctgcccta cctgggtaga aggttttggt accggataat
      781 aagtgattgg gttccagacg atgctgtcat atttgctttg atcaaaactc gggtctaccc
      841 aacgtaaaac aggtttacct gttgctgagg ttgtttcttt taaatcagag taattgttta
      901 aaaaaccaga atatttatct ggctgggtga ttttagaggc acaaccagac agagccaaca
      961 agccgcataa agctgcaact ttcgcaaatg atgtggtacg catgaatagt ttcctgagat
     1021 gattatatta ctttttaagt tatagcaaaa agcgccgctt cgtgttgtgg caaaaaaagt
     1081 gttggtgaaa ataaattttt cagccccttt acagacgaca tagtcaatgc cgaaaaaggg
     1141 gctaagtcaa tcatcagtag ttaaatatca gtgcgttaat ctgatgaaaa acttcgacta
     1201 ctgtgattaa tttcacatcc ttttgattaa aaatatgctt accgaaaaga agaacagtaa
     1261 accatctcag cgtggggaat tttcacgcgc tttgttctta ccggatcatt ttttacgtgc
     1321 cagcatcgtg gcgaaacgca gtttaatacg attaccgttg gcgtcggtgc ggtgcagctc
     1381 gccgacgtcc tcattgtatt tcaccctctc ccagccttcg taatatcgac gtaattctcc
     1441 ctctttgaag gcaaacggga agccgacggt acatggataa tcagcggtat ccatcgccgc
     1501 cacaatcagg ttgtaaccgc caggtttagt gcaacgttgc atattggcaa tcagccctgg
     1561 gatggtttta gcctcaagga acatcagcac cacagtcgaa agaataaaat cgtactgtcc
     1621 atcaaatgtg aggttattca gatcaacgac ccgggtgtgt aaattatcca gattttcaat
     1681 ggacttaatg cgctcgacgt tggcgatact catggcattt ttatcccatg cgtcaacatc
     1741 ataaccattg gctgccaggt aaagactgtt acgaccattg ccacagccca gatccagcgt
     1801 tttacccggt ttaaccactt tcaccgcttc cagcacttca gagtgtgtgc gggttaattc
     1861 atatttatca gtaaaatagt tttcgtcacg aatgatcatt ctttgtcctc tgctttcatt
     1921 aaaacggcgc gctcggttct gactaacaat tttccctgca tcagaagcgc aaaagtacgg
     1981 atgagcagta ttgcaataat aaaattggta aagataaaca gcggcaccgc cagcgtatgg
     2041 aaaaatccat tatcgctgcc actccccaga tgcaaaccgg tggttgccag tgcagatacg
     2101 ccgaacgaga aactccagaa tgaagcatta aatggctggg agagatacca tggcatcaga
     2161 cgtagcataa acagcagttg cagcagtcca taaccgaaaa gcattttcgc cagcgtgtca
     2221 ccctcgccgc cgttgacgct cagccaggca ctacaggcca ccagcgcagg agcgagctga
     2281 atgccgagtg atgtcctcag tgccgtgggt aattctcccg aactgcgcag acgctgcaag
     2341 atcaccggtt ccaggcttag ccatgagaaa acgcctgcgc ctaaaaacac cagaccggcg
     2401 tcggtgtagc ccaacgcacc acaggccatt gcgctgataa agttgttggc aactgtcggc
     2461 agatacagtc caggcgtggt agcttcttca gggtgagatc cgcgccataa tcccgcagtt
     2521 tgccaggcgg cataagccaa ctgaaccacg acaccaaaac tgaacaggca caccgccagt
     2581 gggcgaaacc acggaacaaa accaatcgcc accagcatcg tcgttgccgg aaacaaactc
     2641 acaaaactgc tcagcactgg atggcgcact tctgccagca cgctatgcgg aaagcgtatg
     2701 agtcgggtaa taaatgcgcc agtcaataat ccccagatga tcatcgccag aatcaccagc
     2761 ccatccccca accagtggct gacctgccaa acctggctgg catagcgcca ggcaaatccc
     2821 atccctatcg tccccaacac aataccaaag tagcctgccg gcaaattgag cactttatcg
     2881 ctctgcatct tcttactcat tttgtttaat ttataaagta tatttgaaat gcattttatg
     2941 gaattttctc gagcatagcc agagccgcag aatttgctac ggttatagta agtcacctgt
     3001 gggaaaacgc tatgcgcaaa tatcgtctaa gtgaagaaca gcgagccttc agttatcagg
     3061 aagatggcac taaaaaaaat gtgttattac ggcagattat cgccattagc gattttaacg
     3121 atgtgatagc aggaaccgct ggcggctgga tcgatcgcga aacggtgtta gcacaggaag
     3181 gaaattgctg gatttacgac cagaatgcta ttgcgttcgg cggaacggtg atttccggga
     3241 atacgcgtat caccggcacc agcgtgctgt ggggagaggt ttacgcaacg gataatgtct
     3301 ggatcgataa tagtgaaatc agccagggtg catatataag tgacagtgtc accattagtg
     3361 actcattagt ttgcggtcaa tgtagaatat ttggtcacgc cttaattgat caacattcta
     3421 tgatcgtcgc cgcacaaggc ttaacgcccg accatcaact cctgctacaa atttatgatc
     3481 gcgcgagggt tagcgcctcg cggattgtcc accaggcaca aatttatggc gatgccgtcg
     3541 tccggtatgc ttttattgaa catcgcgccg aagtttttga ttttgccagt attgagggta
     3601 atgaagagaa caacgtctgg ctatgcgatt gtgctaaagt ctacggtcac gcgcaggtga
     3661 aagctggcat agaagaagat gccattccga cgattcatta cagttcgcag gtggcggaat
     3721 atgccattgt ggaaggtaat tgtgtgttaa aacatcatgt cttgattggc ggaaatgccg
     3781 tggtacgtgg cggaccgatt ctgctcgacg agcacgttgt catacaaggt gagagccgta
     3841 ttacaggtgc ggtgataatt gaaaaccatg tggagttaac cgaccacgcc gtcgttgaag
     3901 cctttgatgg cgacacggtt catgtccgtg gtccacaagt cattaacggc gaggagcgca
     3961 ttacgcgaac gccgctggca gggttattgt gaatcgataa tacgcgcgta taagttcaca
     4021 tcatcatagg catcattcag aaactcagcc tgtttcaggc aaccttcaag gataaaacca
     4081 ttgcgcaaag cgacctggtt gctttgcgga ttgtccaccc gacatttgat cacgaagcgt
     4141 ctaagttcac cagactgagc gtaatgatga atcaatgcct gcagcgcctg agaaatgatc
     4201 ccctgcccct gatgagattc gtccagccag tagccgattt cagcggtttt attcagtggt
     4261 tcaatacgat taaacgagat aacgccgata agttcatctt ctttgaaaat catgaacatt
     4321 ttggcatatc cgcgttgatg caacattaca ttgccctgca ccgtttttcg cgtgtcctct
     4381 tcactttgaa caaactgcgg ccagtttagc gactgctgta gccaggttct atttttacag
     4441 attaactgat aaagaggtgt gacatgactt tctgcaacag catgtaattc aagtgattca
     4501 cttactttta tcgtttcggt catcttatct ccagtcactc catgcagttg ttaaaaaagc
     4561 cgcctagctg gcggcttagg aaagtttaca cctctttgac gctctcctgc tgcaggattt
     4621 tgagcatctc atcttttaac tgtagctttt gttttttcat gcggaccact tccgcattgt
     4681 accctcgacc gtcggaacct tcctttctgg caatttcatg atcaagttta ttgtgtttat
     4741 cgaacaagga cataaagcga ggattttcgt ttttcagacg ggatattaaa tctcgatatt
     4801 ctggaaacat acgcactccc tgttagtggc tgacaaggtg tatctgaaat acacaccatc
     4861 agattactca tttaacttag ggaaaaatgc gagcgggatc gtgtttaacc agacggcacc
     4921 tcccgaagaa ggtgccgaag aaaaaacgat taactcatca cgcggtcgtc aacatactga
     4981 cgtttatcgg gcgctggcgg gaaatactga tacagccatg tttcactgat agcgtcgccc
     5041 tgacaacgca gatacatccg catatcgacc ggatcagtgg agtccgaagt cggataccag
     5101 tcaaactgaa tacgataacc atcaatgggt tcaatataga gaatttcgat ttgcttcgct
     5161 tccccactgg aaagcgtaat caccggctca atgccttttg gcgcggcagc tttcagatca
     5221 ccaccaacga aatcgacggc aaaacgacgc gcccattttt cgggatagtg ttcacctgga
     5281 gcccaacctt ccgggaaacc acccatgccg gtacgcgtcg ccataacgcg cgctaatggg
     5341 caatgaacag gcggttgcgc actccagtac agacgatact ggaatgcaaa ctcatcaccc
     5401 gcttttacag ctttttctgg ctgccagaag cagacaatgt tatccagcgt ttcgcccgtt
     5461 gttgggattt ccatcaggcc gatggtgccc ttaccccact tgttacgcgg ttccacccac
     5521 agacttgggc gtttgttata ccagcccata atgtcctgat aatgggagaa atcacgatcc
     5581 agttgcaata aaccaaaccc tttcgggttg ttgtcggtgt aagcattgaa ctgcaatttt
     5641 tgcggattgt tcagcggacg acaaatccac tcgccgttgc cccgccacat ggacaaacga
     5701 tcagagtcat ggatttgcgg atgaatggtg tcgcacatcc gacgttcatt agtaccgcag
     5761 ctgaacatac tggtcatcgg cgcaatgccc agctgtttaa tgtctttgcg cgcatacagg
     5821 tgattttcca catccataat cacctgactt ttctcgcaat ggatagtgaa cttataggca
     5881 ccagtaatgc tggcgctatc gagcaacgca taaacggtaa aggtggttgc ccccggtttt
     5941 accgtatcaa accagaaggc ggtaaagtcg gggaactctt ctttactgtc ggtgtaagtg
     6001 tcgatcgcca ggccgcgagc cgatagaccg tattgatatg tgtcgtcaac ggcgcggaaa
     6061 taactcgcac cgaggaatga tacgacatca cggcgcgcca gttcgggggc tttaaacacg
     6121 cgaaaaccgg caaaaccgag atcgctttgc ccttctaatt gtttggtatc aacacccgca
     6181 tcgttgtatt tgaacaactc cgggcgaaag tgaatttcac gcgccagatg tgttgcggga
     6241 tctacagaaa acatacgaac gcggcgacgg aatcccattc ccatatggaa gaactgagcg
     6301 tccagttgac ggttctcaac gttatgccag agcgattttt cggcgtcgta ttgaatactg
     6361 ttataagcct gcggcgtcat tgtcgccaga gtgtcaggta acggacgcgg cgcaccacgc
     6421 cacgctgttt gcgctaagtc gtgcgccatt gactgtagaa tggagaagtc aaaacgctgg
     6481 gtttgcccgt cggcaatatc agaatctgcc gcgaatgccg cctgagaaaa aagagaagca
     6541 atgccgctgg taccgcacac ggcggccata gccattgaac ctttaataaa tcgtctacga
     6601 tccatacctg aaagtgagtc cttctggtgt tgtgaatggt cccgcgctga acgttttcag
     6661 cggcaagaaa gtgcttattt atgaccgctc actctagaca atatttatta ataatccaat
     6721 tgttgaactg agagaatatg cataaatcgg caattttttt atgtcgatgg gatgagcctg
     6781 attcgtttgt aaaatcaggc aaatttttgc aaaggcggat ggtcagaatt taaagcaaac
     6841 cacagcgacg tttactgcgt tcttccgctt cctgataaag ctggaactca tccaaaaccg
     6901 ggcagcccaa cgcctgctca aatgcttcgc gactggcatt accgtgactg cgtgcctgcg
     6961 tttcattacc cagatttgac tggaagatcc ccgccgcgct aacaggcaag aaatcttcat
     7021 aggtaatggg ttgcgccact acccaaccac gttcaattaa gggctgtgga tcgtctccgg
     7081 gatgaatcgc ctgacgatgc gcctcacctg aaggcgtcag acggtaccgg aaccatgcca
     7141 acccttgctg acgcattaaa aactcactgt caggaaaagt gcggaaggtt tcctgtaaat
     7201 gcatttggtg agtgagatta tcctgcccgg ttccagcgtt ccgcagtaga tcatcataca
     7261 gttgtcgtcc tttcggcgtt aatgccacgc cacgctgctc tatttcacca aagcgcgcgg
     7321 tatgcgtgcc ctgtttctgc cctgcaaaca acaccgtctc ttccagtgct ttaaagctgg
     7381 tctggcgtag taaaatagat acctcgcggc gcggcggccc ttcgatcaga attttgggtt
     7441 caatcccgca ttcaggcatc atcgactgca cccggtcaat atccagcgta cgtggcgtca
     7501 ggtggttgat atggcatcca ggaaaacaga ccacatcagc aatcaaccga tgttcgttgt
     7561 gcaaggcgcg ataggtttct tcatctaccg ttgctgactg gtgccaacga aacgtttcca
     7621 gggcttcctg cacaaactcc tgtgcctgtg tttcgttaaa accgccccgc tgctcatact
     7681 cctctaacag ttgtcgacaa cgtggggtga agatatcgcg ctgacgcaga atctccgccg
     7741 ctttctggcg caaaaattcg ttctcgataa gctcaaggcg gagtaatgag gtaaaaacgc
     7801 ggaagggatt acgcgccaga gaagcatcat caatgggccg aaatgctgtg gaatgtaccg
     7861 gcactcccgc ctgcgagaga tcgtaatagc tcaccgggta catccccata atggcaaaca
     7921 tccgccgaag agtagcaagc tcttgtgcag tcccaacacg aatcgcccca tgacgttcaa
     7981 cattcagtcg cgccagctcg tctgcattta ccattttttc gtgcagttga ggattgtttt
     8041 ccagcacagc cagattcaca tcagctacca gttccagcag cgtgccgtac tgcggaactt
     8101 cttgctggta catggctgac attgcctgcg aaaactgttc ccgaatctca tccgccgtga
     8161 tgctgttcgc catagtgtct gcctccagtg gtcagtaatc tggagtgtag gtaaccgcat
     8221 tcactccggc gggaagaatt tacaaactgt gatctcgccg cgaaaacatc aatattatcc
     8281 attttgctgt aacataattg ctttaattgt taataatatt ttgcaatcaa gttatcataa
     8341 tcaaacaact tcacttgtca gcgacaccgc ttcgttttta acatcgctta tggaaaaaaa
     8401 tagtctgttt agtcagcgca tccgtttgcg ccaccttcat acattcgtag ctgtcgcaca
     8461 acaaggaact ttggggcgcg cggctgaaac ccttaatttg agtcaacctg cgctctctaa
     8521 gacattgaat gaactggagc agctgacggg cgctcgcttg tttgagcgtg gtcgtcaggg
     8581 ggcgcaactt accttacccg gcgaacaatt tttaacgcat gcagtcagag ttcttgacgc
     8641 catcaacact gctggacagt cgcttcatcg taaagaaggt cttaataatg atatcgtcag
     8701 ggttggtgca ctacctactg cggcactggg gatattacct tcggttatag gtcagtttca
     8761 tcagcaacaa aaagagacga ccttgcaagt tgcgacaatg agtaacccta tgattctggc
     8821 gggtttaaaa accggggaaa tcgatatcgg cattggtcgg atgtcagatc ctgaactgat
     8881 gaccgggctt aattacgaac tgctgtttct tgaatcgctg aagctggttg tccgccccaa
     8941 tcatccgcta cttcaggaga acgtaacgct aagccgggtg ctggaatggc cggtcgttgt
     9001 atcaccagaa ggcactgcgc cacgccagca ttcagatgca ttagtgcaga gccagggatg
     9061 taaaattcct tcgggttgta tcgaaacgct gtctgcttcg ctatctcgtc aacttacggt
     9121 tgaatacgac tacgtgtggt ttgtcccttc tggcgctgta aaagacgacc tgcgtcatgc
     9181 cacgctggtg gccctgcctg ttccgggaca tggtgcaggc gaaccgattg gaatactgac
     9241 ccgcgtagat gcgacgttct cttctggttg ccagttgatg attaacgcta ttcgaaaatc
     9301 aatgccgttc tgaaaggtga agggatctgt cgatcccttc cttgaacatt ttcacaccgt
     9361 agcgaaacta actggttcac ccgctccgcg aggttctgcc gacacagaat gtttgttcag
     9421 acggaatacg tccaccgcct cagttaatcg tgccgcctgt tcttcaaggg acaccgccgc
     9481 cgctgaggcc tcttctacca gagaagcatt ctgttgcgtc accttatcca tttcagaaat
     9541 cgcctggcta acctgcgtta tgcctctact ttgttcatcc gaggcggcgg cgatttcctg
     9601 catgatatgt gtgacactcg cgacggcatc aacaatagtg ctcatggttt tccctgccgt
     9661 tgccacctca tccgacccca ggtcaattaa cctgactgat tcactgatca agccttcaat
     9721 ctctttcgcc gcctgagcgc tgcggcttgc gagtgtccgt acttcgctgg cgacaacggc
     9781 aaatccacgt ccttgctcac ccgctcgcgc ggcttcaacg gcagcattca gtgccagaat
     9841 attcgtctgg aaagcaatac tgttgatgac ggcggtgatc tcagaaattt tcttcgaact
     9901 cgtggagata gcgcccatcg tttttactac accggaaacc atctgcccgc catcgctggc
     9961 tttaatagaa gcctcctgcg ccagtttgct ggcatgatgc gcgttatccg cattctgttt
    10021 caccgtcgca gtgagttgct ccatgctggc ggcagtttgt tcgatagccg ccgcttgttc
    10081 ttcggtgcga gatgacaggt ccgcattgcc agctgaaatt tcgctggtgc cacgataaat
    10141 ctcttccgcg ccctgtcgaa cggtccctac agtcatcccc agtgaatgct gcatttgctg
    10201 taaatgacga cttaagcgac cgatttcatt acgacccgcc ggttcatcat tcatcgtcag
    10261 atcgccactg gcgatttttt caatccgttg tgcggcattt tgcagtggac gaatgacgat
    10321 ccgacgtagc accataaatg ttatcagcgt catcaccagg gcaagcacaa acgcgccaat
    10381 catgaacatc ccacccaggc gggtgcgctg atgggccagt tccgccagtt ggttggctct
    10441 ggtgctacgt atcttaacgg ctttgttcaa aatatcggta taagcattat ccagcggtcg
    10501 gatctgctca ctttcatgat tgataatcgc ttcaaacatg ccatttttgg catatttcaa
    10561 catcggttgc atacccgtga tataagcctg gaagcgttga tttaattcag tgtcgagggc
    10621 ttcatcagca ggtgttttca ccggtcgatt ctgataagca cgataacctt gctgcgactg
    10681 tttaatctcc gattcggctt gcgcaatatt tcgcttcatt gcttccattt ccgcaatacg
    10741 actcgctgcc ccggcctgaa tcatattgat ccgggctgaa cgcaaaaaat ctgaactgtt
    10801 cgctaacccg gtccgaatct caatctccgc agtaacatca cgctgatcgc gatcggcctg
    10861 ccagaggaaa tagccagcca gagcagaact taatgcgaat agaactaaga taccgccaag
    10921 aatgcaggca aataacggaa ccagcctgat gtgatgcaaa aaacctaatc gctgtgaggg
    10981 agttgtattc attaccgttg tctctcttcc aggtttactc ctgatagaaa gtcatcggca
    11041 cttgacggta atttcttacg accaaaaacc tctttgcgtc acatttttac aacaattatg
    11101 gcagagaaga ataatgccat tggaatgaag agagtaacga atgattaaac tggctaaacc
    11161 gaatgacgaa aaattctgga aaacagcacg caatttcgtc aggtgattat tttcgtcctt
    11221 aaggaaacca cataggatgc tcacgggtgc ctgagacttt ctgttttcag gctaaccggg
    11281 aatcagaaag acgaaggccc cgaccaatat ttctatcaac cgaggccaac gaatgaacct
    11341 agcaattcag atcatagcct cttacccgcc atccggcaag gagaaaggct atgaagcaca
    11401 accctctggt ggtgtgtctg ctcattatct gcattacgat tctgacattc acactcctga
    11461 cccgacaaac gctctacgaa ctgcggttcc gggacggtga taaggaggtt gctgcgctca
    11521 tggcctgcac gtccaggtaa gggcaagcgc ggggattttc cccgcgcatc tgactagcag
    11581 attcattttc tcatggcacc ccttttacac ttcagttagt gcatgttttt ttcgatgcac
    11641 taatagaacc atcattacag acaaatttgc cgcctgccgt acagtgtgag atcccccctt
    11701 tcgaaccaga acagggtttg cgcccggcat cggcataaaa ggaaacaagc gctcctataa
    11761 ccaagataag tgccagtttt ttcatataac ctccatggtt tatcgaatct tatgttcgat
    11821 ttgaacgtta tacgctggtt tattttgata ccgttgatga gatcaaagat cgggtgaatt
    11881 gattaaagat aagccagatg gacgtatgga ccaggattat ggttttattt gtcatacaaa
    11941 taaatataat aggcgcttcc gatgtagacc cgtattcttc gcctgtacca cgggtcggtt
    12001 ttagtacagg cgttttcttt aaatatcctt caggaacgtt tacgcggcat catgcgtaga
    12061 agtgtgttgt ccttccagaa atagtggtgt gccagtgcag ccgcagcgtg caacccgatg
    12121 acaaaatatc ccagattcgc cagcgtctca tgccaccact ttaagctatc aacccgttcg
    12181 aaattggcct ctgctgcata aggcattgtc ataccaaacg cagaccacgg gttgccccgg
    12241 ttatacatca tcaccaaacc aatcactggc agcgcaataa acaggagata gatcaccaaa
    12301 tgtcccaaat gcgccagtcc cgtcatcatc ggttttggtt taggtacaat cggcggagtt
    12361 gggtatttaa gccttaacaa aagacgaaca accatcagca cgagaattga gatgccacag
    12421 gaaacatgaa tcatgttgat aagcggccta tcgccacgtg ggaaaaaacc acgaaactcc
    12481 attgcgcaat acgctgcgat aaccagcaaa aagaccagcc agtgaatgcc gatttgtaac
    12541 cttgaatatt tactttccat aacatttcct gctttaacat aattttccgt taacataacg
    12601 agcttttctt aaaatttcat taaatattgt tcacccgttt tcagtacgtt acgtttcacg
    12661 gcaagaatgt agattgccca tcccgccatc ctggtctaag cctggaaagg atcaattttc
    12721 atccgaacgt tcctgacagg agaagaccat gagtaaagtt ggtattaacg gttttggtcg
    12781 tatcggtcga ctggtgttgc gtcgattact tgaagtcaaa agcaacatag acattgttgc
    12841 tattaatgat ctcacttccc caaaaattct cgcctacctg ttgaaacatg attcaaacta
    12901 cggaccgttc ccctggagcg ttgattttac ggaagattca cttatcgttg atggaaaaag
    12961 tatcgcggtt tacgccgaaa aagaggctaa aaatattcca tggaaagcga aaggcgcaga
    13021 aatcattgtc gaatgtactg gcttttatac ctccgccgag aaatcgcagg cgcaccttga
    13081 tgctggtgcg aagaaggtgt tgatttccgc ccctgccggt gaaatgaaaa ccatcgttta
    13141 taacgtcaat gacgacacac tggatggcaa cgacaccatt gtttccgtgg cgtcatgcac
    13201 cactaactgt cttgcaccga tggccaaagc cttgcacgac agtttcggaa tagaagtcgg
    13261 cacgatgacg accattcatg cctataccgg cactcagtca ctggtggatg gtccgcgagg
    13321 taaagatcta cgcgcttcac gtgcagcggc agaaaatatc attccccaca ctacaggtgc
    13381 ggcaaaagcc attggtctgg tgatcccgga actgagcggc aaactgaaag gtcatgcgca
    13441 acgcgtgccg gtgaaaacag gttcggtcac tgagctggtg tccattctcg gaaaaaaagt
    13501 gactgccgaa gaggtgaata acgcacttaa acaagcaacc accaataacg agtcatttgg
    13561 ttataccgat gaagaaatag tctcttccga tatcattggc agccatttcg gttcggtgtt
    13621 tgatgccacg caaacggaaa ttaccgccgt gggcgattta caactggtga aaacggtcgc
    13681 ctggtacgat aacgaatatg gcttcgtcac acagctcatt cgcaccctcg aaaaattcgc
    13741 taaactctga cgcacacagg cggaggaaaa acctccgcct cttccactca ttaagactgt
    13801 aaataaacca cctgggtctg cagatattca tgcaggccat gtttaccatc tgcgccgcca
    13861 ataccagatt tacgccatcc ggcgtggaag ccttgcatag cttcgaagtt ttcacggttg
    13921 atgtaagttt caccaaactt cagcccgtta atggctttca tcgcgacgtt cagattttgg
    13981 gtatagattg atgaggtcag gccgtaatca ctatcattcg ccattgagat agcctcttcc
    14041 agcgtatcaa atgcgaccac cggcagaacc gggccaaagg tttcctcatg cataatcgac
    14101 atttcctggc gaacatccag cagcaatgtc ggcggataat aatatccttt cccctctacc
    14161 gctttgccac ccaacgccac tctcgcccct tcttctactg cgcgcgccac tttttgctcg
    14221 accctttcca gcgccgcggc gttaatcaac ggccccatcg caatgtcgtt gcgttcagcg
    14281 gggttaccaa attgaaccgc ctgcaacgct tcacccagcc gattgacaaa ctgatcataa
    14341 atgcctttct gtacataaac acgttctgca cagttacaca cttgcccact attaataacg
    14401 cgtgaatcaa cgatggcttt gactgccagt tcaagatcgg catcgtccat tacgatagcc
    14461 ggtgctttac cccccaattc caggcacact ttggtgatgt ttttcgccgc agtcgccata
    14521 atcttttcac ctgcagagac gctgcctgtc atactgacca ttgcgacctt tgggttaccc
    14581 gccagttctt gcccaacggt ttcaccacgc cccagaacaa ggttaaacac gccgcgcgga
    14641 aggcctattt catcgacaat tttggcgaat gcaatcgcat tgtttggcgt aaattcactg
    14701 ggtttaatga cgatggtatt accggtcaaa agagcggggg ccattttgcg ggcaatgagg
    14761 aagaacggga agttccacgg cagaatgccg gtagtcacgc caagcgcacg tttaaacaga
    14821 agaatatttt ctcctggacg atcgctttga ataatctcgc cttcataacg ccgtgcccac
    14881 tccgccatgt aatcgatata gtccgcagta aaagccactt cgacttcagc cagctgctgg
    14941 atcttgcccc cttcttcaac aatcagcgca ctgatttcgc tggcgcgttt gcggatcccg
    15001 gcggagattt tgcgcaacca actggcgcgt tcaatagcag gcaacgcttc ccattctggt
    15061 tgtgcacgtt ctgctgcatc gattgcctta cggacatcct cggcctgacc atcgggaatg
    15121 cgggaaatga cagcctctgt tgcagggttt accacatcaa tccatgcgtc tccacgccag
    15181 gtaacaaact gtccatcaat atacatagga tgttgaacgg gtactgacat gagcgactcc
    15241 tgtgatttat atgtttgttt ttagtaaata ctttgttaat taacatcaat catcagagag
    15301 acggcatcat gaaaggacaa ttctgtgaag ggcttcataa aaacaagtgt aaaaggaata
    15361 agaggcagaa ataaaaggca ggagttatgc ccgtttgcaa acaagtggtt cacaaacggg
    15421 caatttttaa cgtaacgagc gactttccat cgcctcgatg agcaccgcat cgtgtttcag
    15481 cgtttgccat gcctggatga cttctgccgg aaaatcaacg tgaacgataa aatctcgccc
    15541 gcggggaccg tagccatcgc tatcatcgtg taaacgcggc agctcgccag tgagtagtga
    15601 tagataacgc tcaaccggcc ataatccttg taactgatta ataaagatta cactgtctgc
    15661 gttatttcct aactgaggaa cgaatccggg ataacttaac cagcgtggtg catcgggatt
    15721 gtccccggtc atacggcgga acgtcgccat cgtgcgccgc tgcatggtgg ggtcctgaac
    15781 aacgatagct gtgtgaactc gttctacggc ctgattcaat agcgcgatgc taaagcgtgc
    15841 gttttcaccg cagtttgttg actggtcttc aatccagatt ttttcatgcg gaatgtgcca
    15901 gaactgatga gcgatatccg ccaggatggt agcttcagct ctgccagtgg tgcggatagt
    15961 gttgtagtgc ggatgctgtg cgatggcgct atacaaaaaa gttgtcgagt gaccgatacc
    16021 accactaatc agtaaaggaa tttgctgatc gcgggcaatc ttacatgccg catcgatagt
    16081 cggcataacc gcattgcctg caaggatcac gcaatcggcc tgatacggca cctcaccgga
    16141 gaaatcatcc tgcgccagcc actgaccgat aacatttatg gcatctatgg ttgccggcga
    16201 aagcgtcgga aacggggtaa tgttcatggt tcctccttat cgttccctta agaatactgc
    16261 gtgagataat agtgagaagc ggagcactct gaaaggagga agtcgtattt ccttaactac
    16321 cactccgcaa gcctgacata tcttttaagg aaatgctgta gataaacatt attcatcttg
    16381 cagcagagaa ttgtattttt ctgaactcct gaattttttc ccataccgtt tttagaatta
    16441 ataaaaaacc cggcgctaaa tgcttaccgg gttttagatt tatcaggcaa atagcagtgt
    16501 taaccgctaa tctgctccat cgcctgcaaa atacgcttat ctgaaatcgg ataaggcgta
    16561 ccaagttgtt gagcgaagta actaacgcgc aactcttcta tcatccaacg gatctctttc
    16621 acgtcttcat cctcacgacg tgcgggcggc agtttgttga tccattgctg ccacgcctgc
    16681 tggacgtttt cgactttcag catctgagca cggtcacgat gcggatcaac cgccagtttt
    16741 tccagtcgtt tttcaatcgc ctgcaaataa cgcagcgtgt cgcccaaccg tttgaagccg
    16801 ttaccagtga caaaaccgcg atataccaac ccgcccatct gcgctttaat gtcagaaagc
    16861 cccagcgcca tagtcatatc cacccgccct ttcagacgtt tattgatatt gaacaccgca
    16921 gtgaggattt gctcgacctg cttcgcaata tccaccaccg tgtcgttcag ttcagcacgg
    16981 actttttcat gcagcgcagc aaagccttct tccgtccaga ccgggccacc attggcgtcg
    17041 atcagttgat ccaccccgca ggagatacag tcgtcgatca gctccagcac tttgccatac
    17101 gggttaaagt acagccccag cttggctttg ttcggtaact tttcatgcaa atatttgatc
    17161 ggcgatggaa tattcagcag cagtagacgg cgaagaccgt tccacattgc ctgcttttgt
    17221 tccagcggat tatcaaacaa tttgattgcc acactgtcgc gttcatccac cagcgctggc
    17281 cacgccttca ctttatagtt gccacgcttc tgttcgtagc tttccggcag ctgaccaaaa
    17341 ctccagatat gtaagccgct ctgctcgata ccgtcatccg ccaccgcaga cagcgtttcc
    17401 tgcactttgc ctttcagcgc atctttcaga tcctgtaatg agcgcccttc ttttagcttc
    17461 ttgtttttgt catcc
//
LOCUS       DAMZFT010000103        17454 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.103, whole genome shotgun sequence.
ACCESSION   DAMZFT010000103 DAMZFT010000000
VERSION     DAMZFT010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17454)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17454)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17454
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.103"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..586
                     /locus_tag="PN977_003579"
     CDS             <1..586
                     /locus_tag="PN977_003579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012421685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDI6043580.1"
                     /translation="PDSALASIISAILVIVGQQSIATGIAIALPVAAAGQVLTVFART
                     ITVVFQHAADKAAEEARFRTLDILHVSALGVQALRVAIPALIVSLFVSADMVSNMLSA
                     IPEFVTRGLQIAGGFIVVVGYAMVLRMMGVKYLMPFFFLGFLAGGYLDLSLLAFGGVG
                     VIMALLYIQLNPQWRKAEPHPQTTTITALDQLDD"
     gene            597..1421
                     /locus_tag="PN977_003580"
     CDS             597..1421
                     /locus_tag="PN977_003580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HDI6043581.1"
                     /translation="MEQRKITRSDLVSMFLRSNLQQASFNFERIHGLGFCYDMIPAIK
                     RLYPLKEDQVAALRRHLVFFNTTPAVCGPVIGVTAAMEEARANGAEIDDGTINGIKVG
                     LMGPLAGVGDPLVWGTLRPITAALGASLALSGNILGPLLFFFIFNAVRLAMKWYGLQL
                     GFRKGVNIVSDMGGNLLQKLTEGASILGLFVMGVLVTKWTSINVPLVVSQTPAADGAT
                     VTMTVQNILDQLCPGLLALGLTLLMVRLLNKKINPVWLIFALFGLGIIGNALGFLS"
     gene            1473..2702
                     /gene="sorE"
                     /locus_tag="PN977_003581"
     CDS             1473..2702
                     /gene="sorE"
                     /locus_tag="PN977_003581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-sorbose 1-phosphate reductase"
                     /protein_id="HDI6043582.1"
                     /translation="MKTTALRLYGKRDLRLETFDLPEMQEDEILATVVTDSLCLSSWK
                     EANLGENHKKVPDDVATNPIIIGHEFCGDILAVGKKWQHKFQPGQRYVIQANLQLPDR
                     PDCPGYSFPWVGGEATHVVIPNEVMEQDCLLAYDGETYFEGSLVEPLSCVIGAFNANY
                     HLQEGSYNHTMGIRPQGRTLILGGTGPMGLLAIDYALHGPVNPSLLVITDTDNDKLSY
                     ARKHYPSEPQTLIHYLNATDAAFDTLMALSGGHGFDDIFVFVPNEGLVTLASSLLATD
                     GCLNFFAGPQDKHFSAPINFYDVHYAFTHYVGTSGGNTDDMRAAVKLIEEKKVQAAKV
                     VTHILGLNAAGETTLELPAVGGGKKLVYTGKYLPLTSLTQIQDEELAAILARHQGVWS
                     GEAEQYLLAHAEAISHD"
     gene            2695..3507
                     /locus_tag="PN977_003582"
     CDS             2695..3507
                     /locus_tag="PN977_003582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000610343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate 5-dehydrogenase"
                     /protein_id="HDI6043583.1"
                     /translation="MINRDTQLCMSLAGRPGNFGTRFHNYLYEKLGLNFIYKAFTTQD
                     IAAAVKGIRALGIRGCAVSMPFKESCMPFLDAIDPSAKVIDSVNTIVNDDGRLTGFNT
                     DYIAVKSLITHHQLNTTTRVMIRGSGGMGKAVIAAFRDAGFTDVIIAARNRESGPALA
                     KQYGFQWQPQPEGIACDILVNVTPVGMSGGKESHELAFSEAMVSTASVVFDVVALPPE
                     TPLIRLAQRLDKQTISGAEVIALQAVEQFAMYTGVRPDAQLIAEAAQFAREG"
     gene            3630..4319
                     /gene="pepE"
                     /locus_tag="PN977_003583"
     CDS             3630..4319
                     /gene="pepE"
                     /locus_tag="PN977_003583"
                     /EC_number="3.4.13.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepE"
                     /protein_id="HDI6043584.1"
                     /translation="MELLLLSNSTLPGKAWLEHALPLIAEQLQGRRSAVFIPFAGVTQ
                     TWDDYTAKTAAVLAPLGVSVTGIHSVVDPVAAIENAEIVIVGGGNTFQLLKQCRERGL
                     LAPITDVVKRGALYIGWSAGANLACPTIRTTNDMPIVDPQGFDALNLFPLQINPHFTN
                     ALPEGHKGETREQRIRELLVVAPELTIIGLPEGNWITVSKGHATLGGPNTTYVFKAGE
                     EAVPLEAGHRF"
     gene            complement(4409..6040)
                     /locus_tag="PN977_003584"
     CDS             complement(4409..6040)
                     /locus_tag="PN977_003584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HDI6043585.1"
                     /translation="MLTLLHLLSAVALLVWGTHIVRTGVMRVFGARLRTVLSRSVEKK
                     PLAFCAGIGVTALVQSSNATTMLVTSFVAQDLVALAPALVIVLGADVGTALMARILTF
                     DLSWLSPLLIFIGVIFFLGRKQSRAGQLGRVGIGLGLILLALELIVQAVTPITQANGV
                     QVIFASLTGDILLDALIGAMFAIISYSSLAAVLLTATLTAAGIISFPVALCLVIGANL
                     GSGLLAMLNNSAANAAARRVALGSLLFKLVGSLIILPFVHLLAETMGKLPLPKAELVI
                     YFHVFYNLVRCLVMLPFVDPMARFCKTIIRDEPELDTQLRPKHLDVSALDTPTLALAN
                     AARETLRIGDAMEQMMEGLNKVMHGEPRQEKELRKLADDINVLYTAIKLYLARMPKEE
                     LAEEESRRWAEIIEMSLNLEQASDIVERMGSEIADKSLAARRAFSLDGLKELDALYEQ
                     LLSNLKLAMSVFFSGDVTSARRLRRSKHRFRILNRRYSHAHVDRLHQQNVQSIETSSL
                     HLGLLGDMQRLNSLFCSVAYSVLEQPDEDEGRDEY"
     gene            complement(6260..9943)
                     /gene="metH"
                     /locus_tag="PN977_003585"
     CDS             complement(6260..9943)
                     /gene="metH"
                     /locus_tag="PN977_003585"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HDI6043586.1"
                     /translation="MSSKVEQLRAQLNERILVLDGGMGTMIQSYRLNEADFRGERFAD
                     WPCDLKGNNDLLVLSKPEVIAAIHNAYFEAGADIIETNTFNSTTIAMADYQMESLSAE
                     INFAAAKLARACADEWTARTPEKPRYVAGVLGPTNRTASISPDVNDPAFRNITFDGLV
                     AAYRESTKALVEGGADLILIETVFDTLNAKAAVFAVKTEFEALGVELPIMISGTITDA
                     SGRTLSGQTTEAFYNSLRHAEALTFGLNCALGPDELRQYVQELSRIAECYVTAHPNAG
                     LPNAFGEYDLDADTMAKQIREWAQAGFLNIVGGCCGTTPQHIAAMSRAVEGLAPRKLP
                     EIPVACRLSGLEPLNIGEDSLFVNVGERTNVTGSAKFKRLIKEEKYSEALDVARQQVE
                     NGAQIIDINMDEGMLDAEAAMVRFLNLIAGEPDIARVPIMIDSSKWDVIEKGLKCIQG
                     KGIVNSISMKEGVDAFIHHAKLLRRYGAAVVVMAFDEQGQADTRARKIEICRRAYKIL
                     TEEVGFPAEDIIFDPNIFAVATGIEEHNNYAQDFIGACEDIKRELPHALISGGVSNVS
                     FSFRGNDPVREAIHAVFLYYAIRNGMDMGIVNAGQLAIYDDLPAELRDAVEDVILNRR
                     DDGTERLLELAEKYRGSKTDDTANTQQAEWRSWEVNKRLEYSLVKGITEFIEQDTEEA
                     RQQATRPIEVIEGPLMDGMNVVGDLFGEGKMFLPQVVKSARVMKQAVAYLEPFIEACK
                     EQGKTNGKMVIATVKGDVHDIGKNIVGVVLQCNNYEIVDLGVMVPAEKILRTAKEVNA
                     DLIGLSGLITPSLDEMVNVAKEMERQGFTIPLLIGGATTSKAHTAVKIEQNYSGPTVY
                     VQNASRTVGVVAALLSDTQRDDFVARTRKEYETVRIQHGRKKPRTPPVTLEAARDNDF
                     AFDWQAYTPPVAHRLGVQEVEASIETLRNYIDWTPFFMTWSLAGKYPRILEDEVVGVE
                     AQRLFKDANDMLDKLSAEKMLNPRGVVGLFPANRVGDDIEIYRDETRTHVINVSHHLR
                     QQTEKTGFANYCLADFVAPKLSGKADYIGAFAVTGGLEEDALADAFEAQHDDYNKIMV
                     KALADRLAEAFAEYLHERVRKVYWGYAPNENLSNEELIRENYQGIRPAPGYPACPEHT
                     EKATIWELLEVEKHTGMKLTESFAMWPGASVSGWYFSHPDSKYYAVAQIQRDQVEDYA
                     RRKGMSVSDVERWLAPNLGYDAD"
     gene            10143..10967
                     /gene="iclR"
                     /locus_tag="PN977_003586"
     CDS             10143..10967
                     /gene="iclR"
                     /locus_tag="PN977_003586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate bypass operon transcriptional
                     repressor IclR"
                     /protein_id="HDI6043587.1"
                     /translation="MVAPIPAKRGRKPAVATAPATGQVQSLTRGLKLLEWIAESNGSV
                     ALTELAQQAGLPNSTTHRLLTTMQQQGFVRQVGELGHWAIGAHAFMVGSSFLQSRNLL
                     AIVHPILRNLMEESGETVNMAVLDQSDHEAIIIDQVQCTHLMRMSAPIGGKLPMHASG
                     AGKAFLAQLSEEQVTKLLHRKGLHAYTHATLVSPVHLKEDLAQTRKRGYSFDDEEHAL
                     GLRCLAACIFDEHREPFAAISISGPISRITDDRVTEFGAMVIKAAKEVTLAYGGMR"
     gene            complement(10984..12708)
                     /gene="aceK"
                     /locus_tag="PN977_003587"
     CDS             complement(10984..12708)
                     /gene="aceK"
                     /locus_tag="PN977_003587"
                     /EC_number="2.7.11.5"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001137229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional isocitrate dehydrogenase
                     kinase/phosphatase"
                     /protein_id="HDI6043588.1"
                     /translation="MPRGLELLIAQTILQGFDAQYGRFLEVTSGAQQRFEQADWHAVQ
                     QAMKNRIHLYDHHVGLVVEQLRCITNGQSTDAAFLLRVKEHYTRLLPDYPRFEIAESF
                     FNSVYCRLFDHRSLTPERLFIFSSQPERRFRTIPRPLAKDFHSDHGWESLLMRVISDL
                     PLRLRWQNKSRDIHYIVRHLTETLGTDNLAESHLQVANELFYRNKAAWLVGKLITPSG
                     TLPFLLPIHQTDDGELFIDTCLTTTAEASIVFGFARSYFMVYAPLPAALVEWLREILP
                     GKTTAELYMAIGCQKHAKTESYREYLVYLQGCNEQFIEAPGIRGMVMLVFTLPGFDRV
                     FKVIKDRFAPQKEMSAAHVCACYQLVKEHDRVGRMADTQEFENFVLEKRHISPALMEL
                     LLQEAAEKITDLGEQIVIRHLYIERRMVPLNIWLEQVEGQQLRDAIEEYGNAIRQLAA
                     ANIFPGDMLFKNFGVTRHGRVVFYDYDEICYMTEVNFRDIPPPRYPEDELASEPWYSV
                     SPGDVFPEEFRHWLCADPRIGPLFEEMHADLFRADYWRALQNRIREGHVEDVYAYRRR
                     QRFSVRFV"
     gene            complement(12892..14196)
                     /gene="aceA"
                     /locus_tag="PN977_003588"
     CDS             complement(12892..14196)
                     /gene="aceA"
                     /locus_tag="PN977_003588"
                     /EC_number="4.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase"
                     /protein_id="HDI6043589.1"
                     /translation="MKTRTQQIEELQKEWTQPRWEGITRPYSAEDVVKLRGSVNPECT
                     LAQLGAAKMWRLLHGESKKGYINSLGALTGGQALQQAKAGIEAVYLSGWQVAADANLA
                     ASMYPDQSLYPANSVPAVVERINNTFRRADQIQWSAGIEPGDPRYVDYFLPIVADAEA
                     GFGGVLNAFELMKAMIEAGAAAVHFEDQLASVKKCGHMGGKVLVPTQEAIQKLVAARL
                     AADVTGVPTLLVARTDADAADLITSDCDPYDSEFITGERTSEGFFRTHAGIEQAISRG
                     LAYAPYADLVWCETSTPDLELARRFAQAIHAKYPGKLLAYNCSPSFNWQKNLDDKTIA
                     SFQQQLSDMGYKFQFITLAGIHSMWFNMFDLANAYAQGEGMKHYVEKVQQPEFAAAKD
                     GYTFVSHQQEVGTGYFDKVTTIIQGGTSSVTALTGSTEESQF"
     gene            complement(14226..15827)
                     /gene="aceB"
                     /locus_tag="PN977_003589"
     CDS             complement(14226..15827)
                     /gene="aceB"
                     /locus_tag="PN977_003589"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase A"
                     /protein_id="HDI6043590.1"
                     /translation="MTEQATTTDELAFIRPYGEQEKQILTAEAVEFLTELVTHFTPQR
                     NKLLAARIQQQQDIDNGTLPDFISETASIRDADWKIRGIPADLQDRRVEITGPVERKM
                     VINALNANVKVFMADFEDSLAPDWNKVIDGQINLRDAVNGTISYTNEAGKIYQLKPNP
                     AVLICRVRGLHLPEKHVTWRGEAIPGSLFDFALYFFHNYQALLAKGSGPYFYLPKTQS
                     WQEAAWWSEVFSYAEDRFNLPRGTIKATLLIETLPAVFQMDEILHALRDHIVGLNCGR
                     WDYIFSYIKTLKNYPDRVLPDRQAVTMDKPFLNAYSRLLIKTCHKRGAFAMGGMAAFI
                     PSKDEERNNQVLNKVKADKSLEANNGHDGTWIAHPGLADTAMAVFNDILGSRKNQLEV
                     MREQDAPITADQLLAPCDGERTEEGMRANIRVAVQYIEAWISGNGCVPIYGLMEDAAT
                     AEISRTSIWQWIHHQKTLSNGKPVTKALFRQMLGEEMKVIASELGEERFSQGRFDDAA
                     RLMEQITTSDELIDFLTLPGYRLLA"
     gene            complement(16096..17025)
                     /gene="metA"
                     /locus_tag="PN977_003590"
     CDS             complement(16096..17025)
                     /gene="metA"
                     /locus_tag="PN977_003590"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HDI6043591.1"
                     /translation="MPIRVPDELPAVNFLREENVFVMTTSRASGQEIRPLKVLILNLM
                     PKKIETENQFLRLLSNSPLQVDIQLLRIDSRESRNTPAEHLNNFYCNFEDIQEQNFDG
                     LIVTGAPLGLVEFNDVAYWPQIKQVLEWSKDHVTSTLFVCWAVQAALNILYGIPKQTR
                     TDKLSGVYEHHILHPHALLTRGFDDSFLAPHSRYADFPAALIRDYTDLEILAETEEGD
                     AYLFASKDKRIAFVTGHPEYDAQTLAQEYFRDVEAGLDPEVPYNYFPHNDPQNKPRAS
                     WRSHGNLLFTNWLNYYVYQITPYDLRHMNPTLD"
     gene            17182..>17454
                     /locus_tag="PN977_003591"
     CDS             17182..>17454
                     /locus_tag="PN977_003591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HDI6043592.1"
                     /translation="MVISIRCSRHEEGEELVAIWCRSVDATHDFLSAEYRAELEELVR
                     SFLPEAPLWVAVNERDRPVGFMLLSGQHMDALFIDPDVRGCGVGRML"
BASE COUNT         3972 a         4733 c         4638 g         4111 t
ORIGIN      
        1 tccggattct gcactcgcca gcataatctc cgccattctg gttatcgttg gtcagcaaag
       61 catcgccacc ggaatcgcca tcgcgttgcc tgtggctgcg gcaggccagg tgctgacggt
      121 gtttgcccgt accatcacag tagtgttcca gcacgcggcg gataaagcag cagaagaggc
      181 gcggtttcgc actctcgata ttctgcatgt ctccgcgctt ggcgtgcagg cgctgcgcgt
      241 tgctattccg gcactgattg tctcgctgtt cgtcagcgcc gatatggtga gcaatatgct
      301 gagcgccatt cccgaatttg tgacccgtgg actgcagatt gctggcggtt ttatcgtggt
      361 ggtcggttac gccatggtgc ttcgcatgat gggcgtgaaa tatttgatgc ctttcttttt
      421 cctcggtttc ctcgcaggtg gctacctcga tctcagtctg ctggcgttcg gtggcgtcgg
      481 cgtgatcatg gccctgctct acatccagtt aaatccacag tggcgtaaag ctgaaccaca
      541 tccccagacc accactatca ccgcccttga ccaacttgat gattaacgga gccatcatgg
      601 aacagagaaa aattacacgc agcgatctgg tgagcatgtt tctgcgctcc aacctgcaac
      661 aggcatcctt taactttgaa cgtattcacg ggctgggttt ttgctacgac atgatccccg
      721 ccatcaaacg actttaccca ttaaaagagg atcaggttgc ggcgctcagg cgacacctag
      781 tgttcttcaa taccacgcca gccgtatgtg gcccggtcat cggcgtcact gccgccatgg
      841 aagaggcgcg agccaacggc gcggaaattg atgacggtac cattaacggc atcaaagtcg
      901 gcctgatggg accgttggca ggtgttggcg atccactggt ctggggaacg ctgcgcccga
      961 ttaccgccgc gctcggcgca tctctggcac tttcgggcaa cattctcggc cccctgctgt
     1021 tcttctttat tttcaatgca gtacgtctgg caatgaaatg gtatgggctg caactgggct
     1081 ttcgtaaagg ggtgaatatc gtcagcgata tgggcggtaa tttgctgcaa aaactgaccg
     1141 aaggcgcgtc gattctcggg ctgtttgtaa tgggcgtact ggtgaccaaa tggacatcga
     1201 tcaacgtgcc gttagtggtt tcacaaacgc ctgccgccga tggtgccacc gtcactatga
     1261 ccgtacagaa cattctcgac cagctttgcc ctggattgct ggcgctcggt ttgacgctgc
     1321 tgatggtacg tctgctcaac aaaaaaatta acccggtatg gctgattttc gccctgtttg
     1381 gtttagggat tatcggtaat gcgctgggct tcctgtcctg attcttcgcc ccggcacgac
     1441 tgccggggtc atcgctcaac atgaggtggt ttatgaaaac aacagctctg cgtctttatg
     1501 gcaaacgtga cctgcgtctg gaaacctttg accttcctga aatgcaggag gatgaaatcc
     1561 tcgcgacggt ggtcactgac agcctgtgcc tctcttcctg gaaagaggcc aatctggggg
     1621 aaaaccataa aaaagtaccc gacgatgtgg cgaccaatcc catcatcatc ggccacgaat
     1681 tttgcggcga tattctggcc gtgggtaaaa agtggcagca caaattccag ccgggtcagc
     1741 gttatgtgat tcaggccaac ctgcaactcc ccgaccgccc ggactgcccc ggctactcct
     1801 tcccgtgggt aggcggcgaa gcgacgcatg tggttattcc caacgaggtc atggaacaag
     1861 attgcctgct ggcatacgac ggcgaaacct attttgaagg ctcgctggtt gaaccgcttt
     1921 cctgcgtgat tggcgcgttc aacgccaact atcatcttca ggaaggtagt tataaccaca
     1981 cgatggggat tcgcccgcaa gggcgcacgc tgatcctcgg cggcaccgga ccaatgggac
     2041 tgttggcgat tgattatgcg ctgcatggac ccgttaaccc gtcgctgctc gttattaccg
     2101 ataccgacaa cgacaaattg agttatgcgc gcaagcacta tccatcagaa ccgcaaacac
     2161 tgattcatta tctcaatgcc accgatgcag catttgatac gttaatggcg ctgagtggcg
     2221 gtcacggctt cgatgatatt ttcgtctttg tgcctaatga aggactggtg actctcgcct
     2281 cttccttgct ggcgacagat ggttgcctga atttcttcgc cggaccgcag gataaacatt
     2341 tcagcgcgcc aattaatttc tacgatgtgc attatgcatt tacccactac gtgggcacgt
     2401 caggcggcaa taccgacgac atgcgcgcgg cggtcaaact gatcgaagag aaaaaagtgc
     2461 aggccgcaaa agtggtaaca catattcttg ggctgaatgc cgcgggcgaa accacgcttg
     2521 aattgcctgc cgtcggcggc gggaaaaaac tggtgtatac cgggaaatat ctgccgctga
     2581 catcactcac gcagattcag gatgaagaac tggcggcgat tctggcgcgt catcagggag
     2641 tctggtccgg cgaggcggag cagtacctgc tcgcccatgc agaggcgatt tcccatgatt
     2701 aatcgcgata ctcaactctg catgtcgctg gccggccgcc ccggtaactt cggcacgcgt
     2761 tttcacaatt atctttatga aaagctgggg ctgaatttca tctataaagc gttcaccacc
     2821 caggatattg cagcggcggt gaaagggatt cgcgcgttgg gtattcgcgg ttgcgctgtg
     2881 tcgatgccgt ttaaagaaag ctgcatgcct tttctcgacg cgatcgatcc gtcggcgaag
     2941 gtcattgatt ccgtgaatac catcgtgaat gacgacggca ggctgaccgg gtttaacacc
     3001 gactatatcg cggtgaaaag cctgattacc caccaccagc tcaataccac cacgcgagtg
     3061 atgattcgcg gcagtggcgg catgggcaaa gcggtgattg ccgcctttcg cgacgcgggg
     3121 tttacggatg ttatcattgc cgcccgcaac cgcgagagcg gcccggcgct ggcgaagcag
     3181 tatgggtttc agtggcaacc gcagccggaa ggcatcgcct gcgacattct ggtcaacgtg
     3241 acgcccgttg gcatgagcgg cggtaaagag agccatgagc tggccttcag cgaagcgatg
     3301 gtctcaacgg caagcgtcgt ttttgatgtc gtggccttgc cgccggaaac gccgctcatt
     3361 cgcctggcgc aacggcttga taagcagacg attagcggtg cggaggtgat tgctttacag
     3421 gccgtcgagc agttcgcaat gtataccggc gtacggcctg atgcgcaact gattgctgag
     3481 gcggcacaat ttgcacgaga aggataagaa acatcagcgc cttgtttgcc acgccagaag
     3541 caatgcgcaa catcgtgagc catgtcagta agcggttaaa atcacagtgg tagcatggct
     3601 cccgtgattt cttactaacc ggagaagtaa tggaactgct tttattgagt aactcgacgc
     3661 tgccgggtaa agcctggctg gaacatgcgc tgccgctgat tgctgaacag ttgcagggtc
     3721 gccgttcagc ggtatttatt cctttcgctg gcgtaacgca gacctgggat gattacacag
     3781 cgaaaacggc tgcggttctc gctccgctgg gtgtttctgt caccggtatt catagcgttg
     3841 tcgatcccgt tgccgcgatt gaaaatgctg agatcgtgat tgtcggcggc gggaatactt
     3901 tccagttgct gaaacagtgc cgcgagcgcg ggctgctggc accaattact gacgtggtta
     3961 aacgtggcgc gctgtatatt ggctggagcg caggcgctaa ccttgcttgc ccaaccattc
     4021 gtaccaccaa cgatatgccg attgtcgatc cgcaaggttt cgatgcgctg aatctgttcc
     4081 cgttgcaaat caacccgcac ttcaccaacg cgctgccgga aggccataaa ggtgaaacgc
     4141 gtgagcagcg tattcgcgaa ctgctggtcg tcgcgccaga actcacaatt attggtctac
     4201 cggaaggtaa ctggatcacg gtgagtaaag gtcacgctac gctgggtggc ccgaacacca
     4261 cttatgtgtt taaggctggt gaagaagcgg ttccgctgga agcgggtcac cgtttttaaa
     4321 tgaattgccg gatgcatgat ccggcatctt gttcatcgtg cctgatgcga cgctttacgc
     4381 gtcttatcag gcctacaggg tcacggtatt aatactcgtc ccgtccttcg tcttcatccg
     4441 gctgttccag cacactgtaa gccaccgaac aaaacagcga gttcagacgc tgcatatctc
     4501 ccagtaagcc taaatgtagc gaactggttt caatgctttg cacgttttgc tgatgcaggc
     4561 gatcgacgtg agcatgggaa taacggcgat taagaatgcg aaagcgatgc ttgctgcggc
     4621 gcaaacgacg ggcgctggta acatcgccag agaagaaaac cgacattgcc agttttaaat
     4681 tactgagcaa ttgctcatag agcgcatcca gttccttcaa gccatcaagc gaaaatgccc
     4741 gccgtgctgc cagcgattta tcggcaattt cgctccccat gcgctcgacg atatcggagg
     4801 cctgttcaag gttgagcgac atttcgatga tctccgccca gcggcgcgac tcttcctccg
     4861 ccagctcttc ttttggcatc cgcgccagat acagcttaat ggcggtatag agaacgttga
     4921 tatcatctgc cagcttacgc agctctttct cctgccgtgg ctcgccgtgc atcactttat
     4981 tcagcccttc catcatctgt tccatcgcgt caccaatgcg cagggtttcg cgcgcggcgt
     5041 tcgccagagc aagcgtgggc gtatccagcg cgctgacatc cagatgtttg ggtcgtagct
     5101 gggtatccag ttccggttca tcgcgaataa tcgttttgca aaaccgtgcc atcgggtcaa
     5161 caaatggcag catgaccagg cagcgtacaa ggttgtagaa gacgtggaaa tagatcacca
     5221 gttccgcttt tggcagtggc aacttcccca ttgtctctgc cagcaaatgg acaaacggca
     5281 ggataatcag gctacccacc agcttaaaca gcaggctacc cagcgcgaca cggcgggctg
     5341 cggcattggc ggcactgttg ttgagcatcg ccagcagacc ggaaccgagg ttagcaccaa
     5401 tcaccagaca gagcgccacg gggaaggaga taatgcctgc ggcggtcaga gtcgcggtca
     5461 gcagtacagc agcaaggctg gagtagctga taatggcgaa catcgcgcca atcagcgcat
     5521 ccagcagaat atcgccggtc agcgaggcaa agatcacctg aacgccgttt gcctgggtga
     5581 tcggcgttac ggcctgcaca atcaactcca gcgccagcaa aatcagccca agaccaatac
     5641 cgacgcggcc cagttgcccg gcgcgtgact gtttgcgtcc gaggaaaaaa atcacgccga
     5701 taaaaataag taacggtgac agccaggata agtcgaaggt gagaatacgc gccattagcg
     5761 ccgtcccgac atctgcaccc agcacaatca ccagagccgg tgcgagggct accagatcct
     5821 gagcgacaaa cgaggtcacc agcatggtgg tggcattact gctctgcacc agtgcggtaa
     5881 cgcctatccc cgcgcaaaag gcgagcggct tcttttcgac gcttcggcta agaacagtac
     5941 gcagacgcgc gccgaagacg cgcattacgc ctgttcgaac aatatgagtc ccccagacca
     6001 gcagggcgac ggcagaaagc aggtgaagca gcgttaacac gagttcaggt tctccttatc
     6061 gttatatgtt cctgaagcaa ttgaatgacc aaaggaccgg aaacacgctg actggcatct
     6121 ttactgtgcc ttttgacctt cgtttttccg gctaagatta ttccagcctg tttccagtat
     6181 aagtgtttca tattaataaa gagacagggc actcattgag cgccctgttt gttgtaagga
     6241 aaagtgacag atttgtgaat cagtccgcgt cataccccag attcggtgcc agccagcgct
     6301 caacatctga gacgctcata cctttacggc gggcatagtc ttcaacctga tcgcgctgaa
     6361 tttgcgccac cgcgtagtac ttgctgtccg ggtggctgaa ataccaaccc gaaaccgatg
     6421 caccgggcca catggcgaaa gattctgtga gtttcatgcc agtgtgtttt tccacttcca
     6481 gcagctccca gatggtggct ttttccgtat gttccgggca ggccggatag cccggtgccg
     6541 gacggatgcc ctggtagttt tcgcggatca gttcttcgtt gctgaggttc tcgttcggcg
     6601 catagcccca gtagacttta cgcacacgct catggagata ctccgcaaag gcttccgcca
     6661 gacggtctgc aagggctttc accatgattt tgttgtaatc atcgtgctgc gcttcaaagg
     6721 catcagccag tgcgtcctct tccagcccgc cagtcacggc aaatgcgccg atgtaatctg
     6781 ctttaccaga aagcttcggc gcaacgaagt cagcgagaca gtagttagcg aagccggttt
     6841 tttcggtctg ttggcgcaga tggtggctga cgttgatcac atgggtacgc gtttcgtcac
     6901 ggtagatttc aatgtcatcg cccacacggt ttgccgggaa caggcccacc acgccacgcg
     6961 ggttcagcat tttttcggcg cttaatttat ccagcatgtc gttggcgtct ttaaacagcc
     7021 gctgcgcctc aacacctacc acttcatctt ccagaatgcg cggatacttc ccggccagcg
     7081 accaggtcat aaagaacggc gtccagtcga tgtaattacg cagcgtttcg atgctggctt
     7141 cgacttcctg cacaccgaga cggtgcgcca ccggcggcgt gtaagcctgc cagtcaaaag
     7201 cgaagtcgtt atcacgcgcc gcttccagcg tgaccggtgg tgtgcgtggt ttctttcgtc
     7261 cgtgctgaat acgcacggtt tcgtactcct tgcgggtacg agcaacgaaa tcatcgcgct
     7321 gagtgtcgga aagcaacgcc gccaccacac caacggtgcg tgaagcgttc tgcacataca
     7381 ccgtcgggcc gctgtagttc tgctcaattt tcaccgccgt gtgcgctttt gaggtggtcg
     7441 cgccgccaat caatagcgga atggtgaagc cctgacgctc catctctttc gccacgttaa
     7501 ccatctcgtc cagcgacggc gtgatcagcc ccgaaaggcc aatcaaatca gcattcactt
     7561 ctttagcggt acggagaatt ttttccgcag gcaccatcac gccgagatcg acaatttcgt
     7621 agttgttaca ttgcagcacc acgccaacga tatttttacc gatgtcgtgg acatcgccct
     7681 tcactgtggc gatcaccatc ttgccgttgg ttttaccctg ctctttgcag gcttcaatga
     7741 acggctcgag atacgccacc gcctgtttca tgacgcgcgc cgatttcacc acctgcggca
     7801 ggaacatttt gccctcgcca aacaggtcgc cgaccacgtt catgccgtcc atcaacgggc
     7861 cttcaatcac ttcaatcgga cgcgtagcct gctggcgggc ttcttcggta tcctgctcga
     7921 taaactcggt aatgcctttg accagcgagt attccagacg tttattcact tcccacgaac
     7981 gccactctgc ctgctgggtg ttagcggtgt cgtcggtttt gctgccgcga tatttctcgg
     8041 caagctcgag taaacgctcg gtgccatcgt cgcgacggtt gaggatcaca tcctccaccg
     8101 cgtcgcgcag ttcagcgggt aggtcgtcgt aaatcgccag ctgcccggcg ttgacgatcc
     8161 ccatatccat gccattgcga atagcgtagt agaggaacac cgcgtgaatg gcttcgcgca
     8221 ccggatcgtt gccacggaat gagaaagaaa cgttagaaac gccgccggaa atcagtgcgt
     8281 gcggcagttc acgtttgatg tcttcacacg cgccgataaa gtcctgcgcg taattgttgt
     8341 gctcttcaat gccagttgcg accgcgaaga tgttcgggtc gaagatgata tcttcagccg
     8401 ggaagccaac ctcttcggtg aggattttgt acgcccgacg gcaaatctcg attttccgtg
     8461 cgcgggtatc ggcctggccc tgttcgtcaa aagccattac cacgactgcc gcaccgtaac
     8521 gacgcaacaa tttcgcgtga tggataaaag cgtcgacgcc ttctttcatc gaaatagagt
     8581 taacaatgcc tttgccctgg atacacttca gacctttttc aatgacatcc cattttgagg
     8641 agtcgatcat aatcggcacg cgagcgatat ccggttcacc ggcaatcaga ttgagaaaac
     8701 gcaccatcgc cgcttcggcg tcgagcatcc cttcatccat gttgatatcg ataatctgcg
     8761 caccgttttc cacctgctga cgcgccacat ccagcgcctc gctgtatttc tcttctttga
     8821 tcaggcgctt gaacttagcg gaaccggtga cgttggtgcg ttcccccacg ttcacaaaca
     8881 ggctatcttc gccaatgttc agcggctcca ggccggacaa acggcaggct acggggattt
     8941 ccggcagttt gcgcggcgct aatccttcta ctgcacgact catcgctgca atatgttgtg
     9001 gcgtggtgcc acagcagccg ccgacgatat tgagaaagcc cgcttgcgcc cattcacgta
     9061 tctgttttgc catcgtgtcg gcgtcgagat cgtactcacc aaaggcgttg ggtagcccgg
     9121 cgtttgggtg cgcggtgacg tagcattccg caatccgtga cagctcctgc acgtactggc
     9181 gcagttcgtc tggccccagc gcacagttaa ggccgaaagt cagcgcttcg gcgtggcgaa
     9241 gagagttata aaacgcttcg gtggtctgcc cggagagcgt gcgcccggag gcgtcggtga
     9301 tggtgccgga gatcataatc ggcagctcaa cgcccagcgc ttcaaactcc gttttcaccg
     9361 caaagaccgc cgctttggcg ttaagggtgt cgaaaacggt ttcaatcagg atcagatccg
     9421 cgccaccttc caccagcgct ttggtggact ctcgataagc cgccaccagc ccgtcaaaag
     9481 tgatattacg aaatgccgga tcgttgacgt ccggagaaat agacgccgtg cggttggtcg
     9541 ggccgagaac accggcaacg tagcgcggtt tctctggcgt gcgcgcggtc cactcgtcag
     9601 cacaagctcg cgccagtttc gccgccgcaa agttgatttc cgccgacagg gattccatct
     9661 ggtaatccgc catcgcaatg gtcgtggagt tgaaggtgtt ggtttcgatg atatccgcgc
     9721 ccgcttcaaa gtaggcgttg tggatagcgg cgatcacttc cggtttactg agtaccagca
     9781 ggtcgttgtt gcctttgagg tcgcatggcc agtcggcaaa gcgttcacca cgaaaatcgg
     9841 cttcgttcag tcgatagctc tggatcatgg tgcccatacc gccgtccagc accagaatac
     9901 gttcatttaa ctgcgcacgc agttgttcca ctttgctgct cacacttgct cccgacacgc
     9961 tcaacccaga tttaataaaa attcaacaaa ccatactggc ataaacgcat ctgtggtaaa
    10021 agcgaccacc acgcaacatg agatttgttc aacattaact catcggatca gttcagcaac
    10081 tattgcatta gctaacaata aaaatgaaaa tgatttccac gatgcagaaa aaggagactg
    10141 tcatggtcgc acccattccc gcgaaacgcg gcagaaaacc cgccgttgcc accgcaccag
    10201 cgactggaca ggttcagtct ttaacgcgtg gcctgaaatt actggagtgg attgccgaat
    10261 ccaatggcag tgtggcactc acggagctgg cgcaacaagc cgggttaccc aattccacga
    10321 cccaccgcct gctaaccacg atgcaacagc agggtttcgt gcgtcaggtt ggcgaactgg
    10381 gacactgggc aatcggcgca catgccttta tggtcggcag cagctttctc cagagccgta
    10441 atttgttagc aattgttcac cctatcctgc gcaatctaat ggaagagtct ggcgaaacgg
    10501 tcaatatggc ggtgcttgat caaagcgatc atgaagcgat tattatcgac caggtacagt
    10561 gtacccacct gatgcgaatg tccgcgccta tcggcggtaa attacctatg cacgcttccg
    10621 gtgcgggtaa agccttttta gcccaactga gcgaagaaca ggtgacgaag ctgctgcacc
    10681 gcaaagggtt acatgcctat acccacgcaa cgctagtgtc tcctgtgcat ttaaaagaag
    10741 atctcgccca aacgcgcaaa cggggttatt catttgacga tgaggaacat gcgctggggc
    10801 tacgttgcct tgcagcgtgt attttcgatg agcaccgtga accgtttgcc gcaatttcta
    10861 tttccggacc gatttcacgt attaccgatg accgcgtgac cgagtttggc gcgatggtga
    10921 ttaaagcggc gaaggaagtg acgctggcgt acggtggaat gcgctgactt tttcgcctct
    10981 gatttatacg aatcgaacgc taaaccgctg cctgcgccga tacgcataaa catcttccac
    11041 atgcccttca cgaatgcggt tttgtagtgc gcgccagtaa tcagcgcgga acaggtcggc
    11101 gtgcatctct tcaaacagcg gaccaatacg cgggtcggca catagccagt ggcgaaactc
    11161 ttccgggaaa acatcgcccg gcgagacgct gtaccacggt tcgctggcaa gttcgtcctc
    11221 cgggtagcgc ggcggtggga tgtcgcggaa gttcacttcc gtcatgtagc aaatttcgtc
    11281 gtaatcataa aacaccacac gcccgtgacg ggtgacacca aagtttttaa acagcatgtc
    11341 gccagggaaa atgttagcgg cggcaagctg gcgaatagcg ttaccgtact cttcaatggc
    11401 gtcgcgcagc tgctgacctt ccacttgttc cagccagata ttgagcggca ccatccgccg
    11461 ctcgatataa agatggcgaa tcacaatttg ttcgccgaga tcggtgattt tttccgctgc
    11521 ttcctggagc agtaattcca ttaatgccgg ggaaatatgc cgcttctcca gcacaaagtt
    11581 ttcaaactcc tgggtgtccg ccattcggcc cacacgatcg tgctctttca ccagttggta
    11641 gcaggcacaa acgtgagcgg cagacatctc tttctgcggc gcgaacctgt ctttgatgac
    11701 tttgaagacc cgatcaaaac ccggcaacgt aaacaccagc atcaccatgc cacgaatacc
    11761 cggcgcttca atgaactgct cattacagcc ctgtagataa acgagatatt cgcggtagct
    11821 ttcggtttta gcgtgcttct ggcagccgat agccatatac aattcagcgg tggttttacc
    11881 tggcagaatt tcccgtagcc actcgaccag cgctgcgggc agcggtgcat aaaccataaa
    11941 ataagaacgc gcaaagccaa aaacaatgct cgcttcggcg gtcgtcgtca ggcaagtatc
    12001 aataaataac tcgccgtcgt ccgtctggtg gatcggcagc aaaaatggca atgtgccgga
    12061 aggcgtgatc agtttgccca ccagccaggc ggctttattg cggtaaaaca gttcgttcgc
    12121 cacctgtaaa tgactttccg cgaggttgtc tgtccccagc gtttccgtca gatggcgaac
    12181 gatgtaatga atgtcacggc ttttattctg ccagcgcaga cgcaacggca ggtcgctgat
    12241 aacacgcatc agcagagatt cccagccgtg atcggagtga aagtctttcg ccagcgggcg
    12301 cggaatggta cgaaaacggc gctctggctg ggagctaaag ataaaaagcc gctcgggagt
    12361 aagcgagcgg tggtcaaata accgacagta cacggagtta aaaaagctct ccgcaatctc
    12421 gaagcgcggg taatccggca acagccgcgt gtaatgctct ttgacgcgca gtaaaaatgc
    12481 cgcgtccgtg ctttggccgt tggtaatgca gcgcagttgc tccaccacca gacccacgtg
    12541 atgatcgtaa agatggatac ggtttttcat cgcctgctgg acagcatgcc agtcggcctg
    12601 ttcgaaacgc tgctgcgccc cggaagtcac ttcgaggaat cgaccatact gagcgtcgaa
    12661 gccttgcaga atggtttgtg caatcagtaa ttccaggcca cgcggcattt agcgccctca
    12721 tcaggagcag agaattgccg gatgcggcgt aaacgcctta tccagcctac gttcggcaac
    12781 ggttgtaggc atgataagac gcgcaagcgt cgcatcaggc atcgtgcacc gattgccgga
    12841 tgcggcgtaa atgccttatc cggcctacag tcagcaacgt ttgtaggttg cttagaactg
    12901 cgattcttca gtggagccag tcagtgcggt aaccgacgac gtgccgccct gaataatggt
    12961 cgtcacttta tcgaagtaac ctgtacccac ttcctgctgg tgagatacga aggtatagcc
    13021 atctttcgcg gcggcaaatt ccggctgctg cactttctca acatagtgct tcataccctc
    13081 gccctgggca taggcgtttg ccaggtcaaa catgttgaac cacatgctgt ggatacctgc
    13141 cagggtgatg aactggaact tgtagcccat atccgacagc tgctgctgga agctggcaat
    13201 agttttgtcg tcgaggtttt tctgccagtt gaacgaaggc gagcagttat aagccagcag
    13261 tttgcccgga tatttcgcgt ggatggcttg tgcaaagcga cgcgccagtt ccagatccgg
    13321 cgtggaggtt tcacaccata ccaggtcagc atatggcgca tacgccaggc cacggctgat
    13381 cgcttgttca atacctgcat gagtacggaa gaagccttca ctggtacgct cgccggtaat
    13441 aaattcgctg tcatacgggt cgcaatcgga ggtaatcaga tccgccgcat cagcatcggt
    13501 acgggcaacc agaagggttg gaacgcccgt cacgtcagct gccagacgcg ccgcgaccag
    13561 tttctgaata gcttcctgag ttggcactaa aactttgccg cccatgtgac cgcatttctt
    13621 cactgatgcc agctgatctt cgaagtgaac tgccgctgca ccggcttcaa tcatcgcttt
    13681 catcagttca aaggcattca ggacaccgcc aaaaccggct tccgcatcag caacgatcgg
    13741 caggaagtaa tcgacatagc gcggatcgcc cggctcaatg cccgcggacc attggatctg
    13801 atccgcacgg cggaaggtgt tgttgatccg ctccaccaca gctggcaccg agtttgccgg
    13861 atagagcgac tgatccggat acatgctggc cgccaggtta gcgtccgccg ctacctgcca
    13921 tcccgacaga tagaccgctt caatacccgc tttcgcctgt tgcagcgcct gaccgccagt
    13981 cagtgcaccg aggctgttga tgtagccttt tttcgactcg ccgtgcagca gacgccacat
    14041 tttcgccgcg cccagttgcg ccagcgtgca ttcaggattg actgaaccgc gtaatttcac
    14101 cacatcttcc gcgctgtatg gccgagtaat gccttcccaa cgcggttgag tccactcttt
    14161 ctgtaattct tcaatttgtt gtgtacgggt tttcatgtgc agatgctcca tagttatgtg
    14221 gtggtttacg ctaacaggcg gtagcctggc agggtcagga aatcaattaa ctcatcggaa
    14281 gtggtgatct gttccatcaa acgtgcggca tcgtcaaaac gcccctggga gaaacgttct
    14341 tcgcccagtt cgctggcaat gactttcatc tcttcgccca gcatctggcg gaacaaggct
    14401 ttggtcaccg gtttgccatt gctcaacgtt ttttgatgat ggatccactg ccagattgag
    14461 gtacgggaaa tttcagccgt cgccgcatct tccatcaggc cataaatcgg cacgcagccg
    14521 ttgccggaga tccatgcttc gatgtactgc acagccacgc gaatgttggc gcgcatacct
    14581 tcttcggtgc gttcaccatc acaaggtgcc agcagctgat cggcagtaat cggcgcgtct
    14641 tgttcgcgca tcacttcaag ctgattttta cgggagccga gaatgtcgtt gaataccgcc
    14701 attgccgtat ccgcaagacc tgggtgagcg atccatgtgc catcgtgacc gttattggct
    14761 tccagcgatt tatccgcttt tactttgttg agcacctggt tattgcgctc ttcatctttg
    14821 ctcggaataa acgccgccat gccgcccatc gcaaatgcgc cgcgtttatg gcaggtttta
    14881 atcaacaggc gtgagtaagc attcaggaag ggtttatcca tcgtcactgc ctgtctatct
    14941 ggcaggacgc gatcgggata gtttttcaac gttttgatat aactgaagat gtaatcccaa
    15001 cgaccgcagt tcagaccaac aatatggtca cgcagcgcgt gaaggatttc gtccatctgg
    15061 aacacggcgg gcagcgtttc aatcagcaac gtcgccttga tggtgccgcg cggcagatta
    15121 aagcgatctt ctgcatagct gaagacttcg ctccaccagg ccgcttcctg ccaggactgg
    15181 gttttcggca gatagaaata gggaccgctg ccttttgcta acagagcctg gtagttgtgg
    15241 aagaaataga gcgcaaaatc aaacaggctg ccaggaattg cttcgccgcg ccaggtgaca
    15301 tgtttttccg gcaagtgcag accgcgaacc cgacaaatca acaccgctgg attgggcttg
    15361 agctgataaa ttttgcctgc ttcattggta tagctgatgg tgccgttaac cgcatcgcgc
    15421 aggttaattt gcccgtcgat cactttgttc cagtccgggg ccagtgaatc ttcgaaatcg
    15481 gccataaaga ctttcacatt ggcgttgagc gcgttgatca ccatcttgcg ctcaaccggg
    15541 ccagttatct cgactcgacg atcttgtaag tccgcgggaa taccacgaat tttccagtca
    15601 gcatcacgaa tggaagctgt ttccgaaata aaatcaggca acgttccgtt atcgatatcc
    15661 tgctgctgct gaatgcgcgc tgccagaagt ttattgcgtt gtggcgtaaa atgcgtcacc
    15721 agctcagtca gaaattctac cgcttcggca gtaagaattt gcttctcctg ctcgccatac
    15781 ggccttatga aagccagttc atcggttgtt gttgcctgtt cagtcatcgt gcagctcctc
    15841 gtcatggatc cgaaaacttc cccactgtga acgaaggatc gttgtgcgct tttcgttcag
    15901 cacaactaag actactcatt taaaatgcaa aatcaaaaac aatttccatt ttaattaaaa
    15961 atacttgata acttattgat aacaatatga ataaatttta ttcacctact gaggtgcgtt
    16021 tcggaaataa aaaaggcacc cgaaggtgcc tgaggtaagg tgctgaatcg cttaacgatc
    16081 gactatcaca gaagattaat ccagcgttgg attcatgtgc cgtagatcgt atggcgtgat
    16141 ctggtagacg taatagttga gccagttggt aaacagcaaa ttaccgtgac tacgccagct
    16201 cgctcgcggt ttgttttgcg gatcattgtg cgggaaatag ttatacggta cttccgggtc
    16261 tagtccggct tccacatcgc ggaaatattc ctgcgccagc gtttgcgcat catattcggg
    16321 atggcccgtc acaaaggcaa tgcgcttatc tttgctggca aacagatatg catccccttc
    16381 ttccgtctct gccagaattt ccagatcggt gtaatcacga atcaacgctg ccggaaagtc
    16441 agcatagcgc gaatgcggtg ccaggaatga atcatcaaag ccacgcgtca gaagcgcatg
    16501 aggatggaga atatgatgct cgtaaacgcc agagagtttg tcggtgcgag tttgcttagg
    16561 aatgccgtag aggatattta gcgcggcctg taccgcccag cagacaaaca gcgtcgaggt
    16621 gacgtgatct ttcgaccact ccagcacctg tttgatctgc ggccagtaag cgacatcatt
    16681 aaactccacc aggcccagcg gcgcgccggt aactatcaga ccgtcaaagt tttgttcctg
    16741 aatatcttca aagttacagt agaagttatt cagatgctct gcgggcgtgt tgcgcgattc
    16801 acgggaatca atgcgcaaca gctgaatatc gacctgcaaa ggtgagtttg aaagcaggcg
    16861 cagaaactga ttttcagttt cgatcttctt cggcatcagg ttaaggataa gtacctttag
    16921 cggacgaatt tcctgaccag acgcacgaga agttgtcatc acaaagacgt tttcttcacg
    16981 caagaaattg acggcgggta gctcgtccgg cacacgaatc ggcataacct gattacctca
    17041 ctacatacgc ttatacgttt agacatccag atagctgaag ataaccagaa aatttgccaa
    17101 tgtcgagtct gcatgttgaa ggtgagaaag tttcaaggaa aaggctacgt tagaatataa
    17161 gaatgacgaa aaggagagaa aatggttatt agtattcgct gctcacggca tgaggaaggg
    17221 gaggaactcg ttgcgatttg gtgtcgttct gtcgatgcca ctcacgattt tttatcagca
    17281 gagtatcggg ccgagctgga ggagctagtt cgttccttcc tgccggaagc gccgttgtgg
    17341 gtcgcggtta atgagcggga tcggccggtt gggtttatgt tgttaagtgg gcagcatatg
    17401 gatgcgctgt ttatcgatcc tgatgtgcgc ggctgcggcg tgggtcggat gctg
//
LOCUS       DAMZFT010000104        17430 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.104, whole genome shotgun sequence.
ACCESSION   DAMZFT010000104 DAMZFT010000000
VERSION     DAMZFT010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17430)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17430)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17430
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.104"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(<1..543)
                     /locus_tag="PN977_003592"
     CDS             complement(<1..543)
                     /locus_tag="PN977_003592"
                     /EC_number="1.1.1.79"
                     /EC_number="1.1.1.81"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312465.2"
                     /note="catalyzes the formation of glycolate from
                     glyoxylate and glycerate from hydroxypyruvate; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glyoxylate/hydroxypyruvate
                     reductase B"
                     /protein_id="HDI6043593.1"
                     /translation="MKPSVILYKALPDDLLQRLQEHFTVHQVANLSPQTVEQNAAIFA
                     EAEGLLGSNENVDAALLEKMPKLRATSTISVGYDNFDVDALTARKILLMHTPTVLTET
                     VADTLMALVLSTARRVVEVAERVKAGEWTASIGPDWYGTDVHHKTLGIVGMGRIGMAL
                     AQRAQFGFNMPILYNARRHHK"
     gene            complement(647..1306)
                     /locus_tag="PN977_003593"
     CDS             complement(647..1306)
                     /locus_tag="PN977_003593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family lipoprotein"
                     /protein_id="HDI6043594.1"
                     /translation="MKKRVYLIAAVVSGALAVSGCTTNPYTGEREAGKSAIGAGLGSL
                     VGAGIGALSSSKKDRGKGALIGAAAGAALGGGVGYYMDVQEAKLRDKMRGTGVSVTRS
                     GDNIILNMPNNVTFDSSSATLKPAGANTLTGVAMVLKEYPKTAVNVIGYTDSTGGHDL
                     NMRLSQQRADSVASALITQGVDASRIRTQGLGPANPIASNSTAEGKAQNRRVEITLSP
                     L"
     gene            1459..3792
                     /gene="bisC"
                     /locus_tag="PN977_003594"
     CDS             1459..3792
                     /gene="bisC"
                     /locus_tag="PN977_003594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418007.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin sulfoxide reductase"
                     /protein_id="HDI6043595.1"
                     /translation="MANSSSRYSVLTAAHWGPMLVETDGETVFSSRGALATGMENSLQ
                     SAVRDQVHSNTRVRFPMVRKGFLASPENPQGIRGQDEFVRVSWDEALELIHHQHKRIR
                     EAYGPASIFAGSYGWRSNGVLHKASTLLQRYMALAGGYTGHLGDYSTGAAQAIMPYVV
                     GGSEVYQQQTSWPLVLEHSDVVVLWSANPLNTLKIAWNASDEQGLSYFSALRDSGKKL
                     ICIDPMRSETVDFFGDKMEWVAPHMGTDVALMLGIAYTLVENGWHDEAFLARCTTGYA
                     VFASYLLGESDGIAKNAEWAAEICGVGAAKIRELAALFHQNTTMLMAGWGMQRQQFGE
                     QKHWMIVTLAAMLGQIGTPGGGFGLSYHFANGGNPTRRAAVLSSMQGSLPGGTDAVDK
                     IPVARIVEALENPGGAYQHNGMDRHFPDIRFIWWAGGANFTHHQDTNRLIRAWQKPEL
                     VVISECFWTAAAKHADIVLPATTSFERNDLTMTGDYSNQHLVPMKQVVPPRYEARNDF
                     DVFAELSERWEKGGYARFTEGKSELQWLETFYNVARQRGASQQVELPPFAEFWEANQL
                     IEMPENPDSERFIRFADFRRDPQAHPLKTASGKIEIFSQRIADYAYPDCPGHPMWLEP
                     DEWQGNAEPEQLQVLSAHPAHRLHSQLNYSSLRELYAVANREPVTIHPDDAQARGIQD
                     GDIVRLWNARGQILAGAVISEGIKPGVICIHEGAWPDLDLTADGICKNGAVNVLTKDL
                     RSSRLGNGCAGNTALAWLEKYNGPELTLTAFEPPASS"
     gene            complement(3761..4201)
                     /locus_tag="PN977_003595"
     CDS             complement(3761..4201)
                     /locus_tag="PN977_003595"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HDI6043596.1"
                     /translation="MIREAQRSELPAILELWLESTTWGHPFIKANYWRDCIPLVRDAY
                     LANAQNWVWEEDGKLLGFVSIMEGRFLAAMFVAPKAVRRGIGKALMQYVQQRHPHLML
                     EVYQKNQPAIDFYHAQGFHIVDCAWQDETQLPTWIMSWPVVQTL"
     gene            complement(4198..4761)
                     /gene="tag"
                     /locus_tag="PN977_003596"
     CDS             complement(4198..4761)
                     /gene="tag"
                     /locus_tag="PN977_003596"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HDI6043597.1"
                     /translation="MERCGWVSQDPLYIAYHDNEWGVPETDSKKLFEMICLEGQQAGL
                     SWITVLKKRENYRASFHQFDPVKVAAMQEEDVERLVQDAGIIRHRGKIQAIIGNARAY
                     LQMEQNGEPFADFVWSFVNHQPQVTQATTLSEIPTSTPASDALSKALKKRGFKFVGTT
                     ICYSFMQACGLVNDHVVGCCCYPGNKP"
     gene            4919..5617
                     /locus_tag="PN977_003597"
     CDS             4919..5617
                     /locus_tag="PN977_003597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312460.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HDI6043598.1"
                     /translation="MIIKKSGGRWQLSLLVSVVISAFFLNTAYAWQQEYIVDTQPGHS
                     TERYTWDSDHQPDYNDILSQRIQSSQRALGLEVNLAEETPVDVTSSMSMGWNFPLYQQ
                     VTTGPVAALHYDGTTTSMYNEFGDSTTTLTDPLWHASVSTLGWRVDSRLGDLRPWAQI
                     SYNQQFGENIWKAQSGLSRMTATNQNGNWLDVTVGADMLLNQNIAAYAALSQAENTTN
                     NSDYLYTMGVSARF"
     gene            5846..7054
                     /locus_tag="PN977_003598"
     CDS             5846..7054
                     /locus_tag="PN977_003598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDI6043599.1"
                     /translation="MTPSNYQRTRWLTLIGTIITQFALGSVYTWSLFNGALSAKLGAP
                     VSQVAFSFGLLSLGLAISSSVAGKLQERFGVKRVTMASGILLGLGFFLTAHSNNLMML
                     WLSAGVLVGLADGAGYLLTLSNCVKWFPERKGLISAFAIGSYGLGSLGFKFIDTHLLE
                     TVGLEKTFVIWGAIVLVMIVFGATLMKDAPKQEVKTSNGVVEKDYTLAESMRKPQYWM
                     LAVMFLTACMSGLYVIGVAKDIAQSLAHLDAISAANAVTVISIANLSGRLVLGILSDK
                     IARIRVITIGQVISLVGMAALLFAPLNAVTFFAAIACVAFNFGGTITVFPSLVSEFFG
                     LNNLAKNYGVIYLGFGIGSICGSIIASLFGGFYVTFYVIFALLILSLALSTTIRQPEQ
                     KVLREAHGSL"
     gene            7378..9069
                     /gene="eptB"
                     /locus_tag="PN977_003599"
     CDS             7378..9069
                     /gene="eptB"
                     /locus_tag="PN977_003599"
                     /EC_number="2.7.8.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418002.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid A phosphoethanolamine
                     7''-transferase"
                     /protein_id="HDI6043600.1"
                     /translation="MRYIKSITQQKLSFLLAIYIGLFMNGAVFYRRFGSYAHDFTVWK
                     GISAVVELAATVLVTFFLLRLLSLFGRRSWRILASLVVLFSAGASYYMTFLNVVIGYG
                     IIASVMTTDIDLSKEVVGLNFILWLIAVSALPLILIWNNRCRYTLLRQLRTPGQRIRS
                     LAVVVLAGIMVWAPIRLLDIQQKKVERATGVDLPSYGGVVANSYLPSNWLSALGLYAW
                     ARVDESSDNNSLLNPAKKFTYQAPQNVDDTYVVFIIGETTRWDHMGIFGYERNTTPKL
                     AQEKNLAAFRGYSCDTATKLSLRCMFVRQGGAEDNPQRTLKEQNIFAVLKQLGFTSDL
                     YAMQSEMWFYSNTMADNIAYREQIGAEPRNRGKPVDDMLLVDEMQQSLGRNPDGKHLI
                     ILHTKGSHFNYTQRYPRSFAQWKPECIGVDSGCTKAQMINSYDNSVTYVDHFISSVID
                     QVRDKKAIVFYAADHGESINEREHLHGTPRELAPPEQFRVPMMVWMSDKYLENPVNAQ
                     AFAQLKKEADMKVPRRHVELYDTIMGCLGYTSPDGGINENNNWCHIPQAKEAAAN"
     gene            9161..9237
                     /locus_tag="PN977_003600"
     tRNA            9161..9237
                     /locus_tag="PN977_003600"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9195..9197,aa:Pro,seq:cgg)
     gene            9981..11588
                     /gene="dppA"
                     /locus_tag="PN977_003601"
     CDS             9981..11588
                     /gene="dppA"
                     /locus_tag="PN977_003601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter substrate-binding
                     protein DppA"
                     /protein_id="HDI6043601.1"
                     /translation="MRISLKKSGMLKLGLSLVAMTVAASVQAKTLVYCSEGSPEGFNP
                     QLFTSGTTYDASSVPLYNRLVEFKIGTTEVIPGLAEKWEVSEDGKTYTFHLRKGVKWH
                     DNKEFKPTRELNADDVVFSFDRQKNAQNPYHKVSGGSYEYFEGMGLPELISEVKKVDD
                     NTVQFVLTRPEAPFLADLAMDFASILSKEYADAMMKAGTPEKLDLNPIGTGPFQLQQY
                     QKDSRIRYKAFDGYWGTKPKIDTLVFSITPDASVRYAKLQKNECQVMPYPNPADIARM
                     KQDKSINLMEMPGLNVGYLSYNVQKKPLDDVKVRQALTYAVNKDAIIKAVYQGAGVSA
                     KNLIPPTMWGYNDDVQDYTYDPEKAKALLKEAGLEKGFSIDLWAMPVQRPYNPNARRM
                     AEMIQADWAKVGVQAKIVTYEWGEYLKRAKDGEHQTVMMGWTGDNGDPDNFFATLFSC
                     AASEQGSNYSKWCYKPFEDLIQPARATDDHNKRVELYKQAQVVMHDQAPALIIAHSTV
                     FEPVRKEVKGYVVDPLGKHHFENVSIE"
     gene            11739..12758
                     /gene="dppB"
                     /locus_tag="PN977_003602"
     CDS             11739..12758
                     /gene="dppB"
                     /locus_tag="PN977_003602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppB"
                     /protein_id="HDI6043602.1"
                     /translation="MLQFILRRLGLVIPTFIGITLLTFAFVHMIPGDPVMIMAGERGI
                     SPERHAQLLAELGLDKPMWQQYLHYIWGVMHGDLGISMKSRIPVWEEFVPRFQATLEL
                     GVCAMIFATAVGIPVGVLAAVKRGSIFDHTAVGLALTGYSMPIFWWGMMLIMLVSVHW
                     NLTPVSGRVSDMVFLDDSNPLTGFMLIDTAIWGEDGNFIDAVAHMILPAIVLGTIPLA
                     VIVRMTRSSMLEVLGEDYIRTARAKGLTRMRVIIVHALRNAMLPVVTVIGLQVGTLLA
                     GAILTETIFSWPGLGRWLIDALQRRDYPVVQGGVLLVATMIILVNLLVDLLYGVVNPR
                     IRHKK"
     gene            12768..13670
                     /gene="dppC"
                     /locus_tag="PN977_003603"
     CDS             12768..13670
                     /gene="dppC"
                     /locus_tag="PN977_003603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010426221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppC"
                     /protein_id="HDI6043603.1"
                     /translation="MSQVTENKVISAPVPMTPLQEFWHYFKRNKGAVVGLVYVVIVLF
                     IAIFANWIAPYNPAEQFRDALLAPPAWQEGGSMAHLLGTDDVGRDVLSRLMYGARLSL
                     LVGCLVVVLSLIMGVILGLIAGYFGGLVDNIIMRVVDIMLALPSLLLALVLVAIFGPS
                     IGNAALALTFVALPHYVRLTRAAVLVEVNRDYVTASRVAGAGAMRQMFINIFPNCLAP
                     LIVQASLGFSNAILDMAALGFLGMGAQPPTPEWGTMLSDVLQFAQSAWWVVTFPGLAI
                     LLTVLAFNLMGDGLRDALDPKLKQ"
     gene            13681..14664
                     /gene="dppD"
                     /locus_tag="PN977_003604"
     CDS             13681..14664
                     /gene="dppD"
                     /locus_tag="PN977_003604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HDI6043604.1"
                     /translation="MALLNVDKLSVHFGDESAPFRAVDRISYSVKQGEVVGIVGESGS
                     GKSVSSLAIMGLIDYPGRVMAEKLEFNGQDLQRISEKERRNLVGAEVAMIFQDPMTSL
                     NPCYTVGFQIMEAIKVHQGGNKSTRRQRAIDLLNQVGIPDPASRLDVYPHQLSGGMSQ
                     RVMIAMAIACRPKLLIADEPTTALDVTIQAQIIELLLELQQKENMALVLITHDLALVA
                     EAAHKIIVMYAGQVVETGDAHAIFHAPRHPYTQALLRALPEFAQDKERLASLPGVVPG
                     KYDRPNGCLLNPRCPYATDKCRAEEPALNMLADGRQSKCHYPLDDAGRPTL"
     gene            14661..15665
                     /gene="dppF"
                     /locus_tag="PN977_003605"
     CDS             14661..15665
                     /gene="dppF"
                     /locus_tag="PN977_003605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP binding subunit
                     DppF"
                     /protein_id="HDI6043605.1"
                     /translation="MSTQEATSQQPLLQAIDLKKHYPVKKGMFAPERLVKALDGVSFN
                     LERGKTLAVVGESGCGKSTLGRLLTMIETPTGGELYYQGQDLLKHDPQAQKLRRQKIQ
                     IVFQNPYGSLNPRKKVGQILEEPLLINTSLSKDQRREKALSMMAKVGLKTEHYDRYPH
                     MFSGGQRQRIAIARGLMLDPDVVIADEPVSALDVSVRAQVLNLMMDLQQELGLSYVFI
                     SHDLSVVEHIADEVMVMYLGRCVEKGTKDQIFNNPRHPYTQALLSATPRLNPDDRRER
                     IKLTGELPSPLNPPPGCAFNARCRRRFGPCTQLQPQLKDYGGQLVACFAVDQDENPQR
                     "
     gene            complement(15695..16966)
                     /locus_tag="PN977_003606"
     CDS             complement(15695..16966)
                     /locus_tag="PN977_003606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease"
                     /protein_id="HDI6043606.1"
                     /translation="MQHNTLPKHDQKLPFTRYDFGWVLLCIGMAIGAGTVLMPVQIGL
                     KGIWVFITAAIIAYPATWVVQDIYLKTLSESDSCNDYTDIISHYLGKNWGIFLGVIYF
                     LMIIHGIFIYSLSVVFDSASYLKTFGLTDADLSQSLLYKVAIFAVLVAIASGGERLLF
                     KISGPMVVVKVGIIVVFGFAMIPHWNFANITAFPQASVFFRDVLLTIPFCFFSAVFIQ
                     VLNPMNIAYRKREADKVLATRLALRTHRISYVTLIAVILFFAFSFTFSISHEEAVSAF
                     EQNISALALAAQVIPGHIIHITSTVLNIFAVLTAFFGIYLGFHEAIKGIILNLLSRII
                     DTKKINSRVLTLAICAFIVITLTIWVSFRVSVLVFFQLGSPLYGIVSCLIPFFLIYKV
                     AQLEKLRGFKAWLILLYGILLCLSPLLKLIE"
BASE COUNT         4094 a         4342 c         4673 g         4321 t
ORIGIN      
        1 tttatggtgg cgacgtgcgt tatagaggat gggcatgttg aagccaaatt gcgcacgttg
       61 tgccagcgcc atgccgatcc gtcccatccc gacaatgccc agtgttttat ggtgaacgtc
      121 agtgccgtac cagtccgggc ctatgctcgc ggtccattcg cctgctttta cccgttctgc
      181 tacctccaca acccgacgag cggtagacaa caccagcgcc atcagcgtat cggcgacggt
      241 ttctgttaat acggttggtg tgtgcatcag cagaattttt cgggcggtaa gcgcatcgac
      301 atcaaaattg tcatagccga cggagatcgt tgatgtggca cgcagtttcg gcattttttc
      361 cagcaatgcg gcatcaacat tctcatttga acccagcaag ccttcagctt cggcaaaaat
      421 tgctgcattt tgttcgacgg tttgtgggct gaggtttgcc acctggtgaa cggtgaaatg
      481 ttcttgcagg cgttgcagta aatcatcagg taaggctttg tagaggataa cggacggctt
      541 catgcttctc tccattcact gataaggcga tcaccttagc ctgaaatatg atgaaggaaa
      601 actgaagaat gccaggtgat ttctgtatca cctgacatga aagggattac agtgggctta
      661 aggtaatttc tacacggcgg ttttgcgcct taccttctgc ggtgctgttg ctggcgattg
      721 ggttagccgg gccaaggcct tgagtacgga tgcggctggc gtccacgccc tgggtgatca
      781 acgcgctggc aacggaatcc gcacgctgct gggagagacg catgttcagg tcgtgccccc
      841 ccgtgctgtc ggtataacca atcacgttaa ccgccgtttt cggatactct ttcagtacca
      901 ttgccacgcc ggtcagggtg ttagcgcccg ccggtttcag ggttgcgctg ctgctgtcga
      961 aggtcacatt gttcggcata ttgaggataa tgttatcccc gctgcgggtt acgctaacac
     1021 cagtgccgcg cattttgtca cgcagcttcg cttcctgcac atccatgtaa taaccaacgc
     1081 cgccgcccag agctgcgcct gctgctgcgc caatgagcgc gcctttaccg cgatctttct
     1141 tcgaagaaga gagcgcacca atacccgcgc caacgagaga gcccagacct gcgccgatag
     1201 cagatttacc tgcttcgcgt tcgccggtgt aagggttagt tgtacagcca gataccgcca
     1261 gagcgccact cactacggcg gcaataagat aaacacgttt cttcattgtt aatccttaat
     1321 aaccttttta ttctttgcca cgggttccgt ggcgggagat tatgccgcgt gaacatgaag
     1381 attattcctg ggaatactcg gaaatttgta agtaatattt aactgctcaa tacatctaac
     1441 ctttcaggag tcttcggttt ggccaactca tcctcacgat attccgttct gactgccgcc
     1501 cactgggggc ctatgctggt tgaaaccgac ggcgaaaccg tgtttagctc gcgtggcgcg
     1561 ttagccacag gaatggaaaa ctccttgcag agcgcggttc gcgaccaggt tcacagcaat
     1621 acgcgggtgc gatttccaat ggtgcgcaaa ggctttcttg cgtcaccgga aaatccgcaa
     1681 ggcattcgtg ggcaggatga atttgttcgc gtgagttggg atgaggcgct ggagcttatt
     1741 caccatcaac ataaacgcat tcgtgaggct tatggtccgg catcgatttt tgcgggttcc
     1801 tacggctggc gttcaaacgg cgtgctgcat aaggcctcga cattattaca acgctatatg
     1861 gcgctggcag gcggttatac tgggcatctg ggggattatt cgaccggcgc ggcacaggcg
     1921 atcatgccgt atgtcgtggg gggcagcgaa gtttatcaac agcaaaccag ttggccgctg
     1981 gtgctggaac atagcgatgt cgtggtgctg tggagtgcta acccactcaa tacgctgaaa
     2041 attgcgtgga atgcatccga tgagcagggg ctttcttact tttccgcact gcgtgacagc
     2101 gggaaaaagc tgatctgcat tgatccaatg cgatcggaaa ccgtcgattt ctttggcgat
     2161 aaaatggaat gggtggcacc gcacatgggc accgatgttg cgctgatgct ggggatcgcc
     2221 tatacgctgg tggaaaatgg ttggcacgac gaagcgtttc tggcgcgttg caccacaggt
     2281 tatgccgtct tcgcctctta tttgctgggc gagagtgacg gaatagcgaa aaacgccgaa
     2341 tgggcggcag agatttgtgg tgttggcgca gcgaaaatcc gcgagctggc ggctcttttc
     2401 caccaaaata ccaccatgct gatggctggc tggggaatgc aacgtcaaca gtttggcgag
     2461 caaaagcact ggatgatcgt cacgctggcg gcaatgttgg ggcaaatcgg cacacccggc
     2521 ggcggttttg gtctttctta ccattttgcc aatggtggta accccacgcg ccgcgctgcg
     2581 gtgctctctt ccatgcaggg tagcttgcct ggcggcaccg atgcggtgga taaaatccct
     2641 gttgcccgca ttgttgaagc actggaaaac cccggtggcg catatcaaca taacggtatg
     2701 gaccgacatt tcccggatat tcgttttatc tggtgggcgg gcggtgccaa ctttactcat
     2761 catcaggata ccaatcgcct gatccgtgcc tggcaaaaac cggagctggt ggtgatctct
     2821 gaatgcttct ggacggctgc ggcgaaacac gcggatatcg ttctgcctgc gaccacttcg
     2881 tttgagcgta atgatctcac catgaccggc gattacagta atcagcatct ggtgccgatg
     2941 aagcaagtgg tgcctccacg ttatgaagcg cgtaatgatt ttgatgtctt tgccgagttg
     3001 agtgaacgtt gggagaaggg cggctatgcg cggtttacgg aaggaaaaag tgagctgcaa
     3061 tggctggaaa cgttttataa cgttgcccgg cagcgcgggg caagccagca ggttgaattg
     3121 ccgccatttg ctgagttctg ggaagccaac cagttaattg agatgccgga aaacccggac
     3181 agcgagcggt ttattcgctt cgccgatttt cgccgcgatc cgcaggcgca tccattaaaa
     3241 accgccagcg gtaagattga aatcttctcg cagcgtattg ccgattacgc ttatccggat
     3301 tgccctgggc atccaatgtg gctggagccg gacgaatggc agggcaatgc cgaaccggaa
     3361 cagttgcagg tactttctgc tcatccggca catcgtctgc acagccagct gaattacagt
     3421 tctctgcgcg aattgtacgc ggtggcaaat cgtgagcctg tcaccattca tcctgacgat
     3481 gcccaggcgc gcggcataca agatggcgat attgttcggt tgtggaacgc acgcgggcaa
     3541 attcttgccg gagcggtcat tagcgaggga attaaacctg gcgtgatttg cattcatgaa
     3601 ggggcatggc cggatctgga tttaaccgct gacggtattt gtaaaaacgg cgcggtgaac
     3661 gttctgacca aagatctccg cagctcgcgg ctggggaatg gctgtgcggg taatacggcg
     3721 ctggcatggc tggaaaaata caacggtccg gaactgacac ttacagcgtt tgaaccaccg
     3781 gccagctcat aatccaggtg ggtagttggg tttcatcttg ccatgcgcag tcgacaatgt
     3841 gaaaaccctg cgcatggtaa aaatctatcg ccggttgatt tttttgataa acctccagca
     3901 tcagatgggg atggcgctgc tgcacatact gcattagcgc cttaccaata ccgcgtctga
     3961 cggccttcgg tgcgacaaac attgcagcca gaaaccggcc ttccataatg ctgacaaaac
     4021 cgagaagctt accgtcttct tcccagaccc agttttgcgc gttggcaaga taggcatccc
     4081 gcaccagcgg aatgcaatca cgccagtaat tcgcttttat aaagggatgc ccccaggttg
     4141 tactttccag ccacagttcg aggatcgcgg ggagttctga acgttgcgct tcccgaatca
     4201 tggtttattt cccggatagc aacagcagcc aaccacatga tcattcacca gcccacatgc
     4261 ctgcataaag gagtaacaga ttgtggtacc aacaaactta aaaccacgct ttttcagggc
     4321 tttagataga gcgtcggagg cgggcgtaga tgtagggatt tcgctcaacg ttgtggcttg
     4381 tgtcacctgt ggttgatgat tgacaaacga ccagacaaag tcggcaaacg gttcgccgtt
     4441 ctgttccatt tgcaggtacg cccgcgcatt accaataatt gcctgaattt tcccgcgatg
     4501 gcggataatc ccggcgtcct gtaccagtct ttcgacatcc tcttcctgca ttgctgccac
     4561 cttcaccgga tcgaactgat gaaagctggc gcgatagttt tcgcgttttt tgaggacggt
     4621 gatccacgat aatccagcct gctgcccttc caggcagatc atttcgaaca gttttttact
     4681 gtcagtttca ggcacgcccc attcattatc atggtaggca atataaagcg gatcctgact
     4741 cacccagccg caacgttcca tactttccct cgctatgtac ccgatcgtaa aaatttcagc
     4801 cgcttttctt gacgcatcgg tgaaaaagtc aatatttaat acagggctac tatgcccgat
     4861 atccttcata caatgacaat aatattgccg aattcggctc ttccctggag tcgtgttaat
     4921 gatcataaaa aaaagcggtg gtcgctggca gctaagcctg ctggtgagcg tggtaatcag
     4981 tgcctttttt ctcaacacag cttacgcctg gcaacaagaa tatatcgttg atacgcaacc
     5041 cggacattcc acagagcgtt acacctggga tagcgatcat caacctgatt acaacgatat
     5101 tttgtcgcaa cgtattcaaa gtagccaaag ggcgctggga ctggaagtca atctggcgga
     5161 agaaacccct gtggatgtca ccagtagtat gagtatgggg tggaattttc ctttatatca
     5221 acaggttaca accggtccgg tcgcggcatt acattacgat ggcacaacca cctcgatgta
     5281 taacgagttt ggcgacagta ctaccacgct gaccgatccg ttatggcatg ccagcgtgag
     5341 tactttaggc tggcgtgttg actcccggct tggcgatctc cgaccctggg cgcaaatcag
     5401 ctataaccag caatttggcg agaatatctg gaaggcgcaa tcaggcctga gccggatgac
     5461 ggcgacaaac cagaacggca actggctgga tgtcaccgta ggcgctgata tgttgctcaa
     5521 tcaaaatatt gctgcctatg cggcgctatc tcaggcagaa aataccacta acaatagcga
     5581 ctatctgtat acgatggggg ttagtgccag attttaacgt tatagataca attgaaaccc
     5641 ataaataaca actaatgtgg cgatagtggg gactaactta ctaaataata aatttggcga
     5701 ataattgtcg agtcattcat tcctgaacta atgcatttca ttccgttctg atggcatttc
     5761 atgccgtttt tccccaggca taaagcgcac ttcgttatgg ttgtcagcag agatttttcc
     5821 tttttattac tgcaggaata ctgccatgac accttcaaat tatcagcgta ctcgctggct
     5881 gacactcatc ggtactatca ttacccagtt tgctctgggg tcggtttata cctggagtct
     5941 gtttaacggt gcgctttctg ccaagctggg ggctcctgta agccaggttg ctttctcttt
     6001 cggcttgtta agtctggggc tggcaatttc atcttctgtt gcgggcaaat tgcaggaacg
     6061 ttttggtgtt aaacgcgtca ctatggcttc cggcattttg ctgggattag gtttcttcct
     6121 gacagcacat tccaacaacc tgatgatgct gtggttaagc gccggtgtgt tggttggtct
     6181 ggcggatggt gcgggttacc tgttgacgct ctctaactgc gtgaagtggt ttccggagcg
     6241 taagggactt atctctgctt ttgctatcgg ttcttatggt ctgggcagcc tgggttttaa
     6301 atttatcgac acgcacctgc tcgaaacggt gggtctggaa aaaacctttg ttatttgggg
     6361 cgcgattgta ctggtgatga ttgtctttgg cgcaacgtta atgaaagatg cgccgaagca
     6421 ggaagtgaaa accagcaatg gtgtggtgga gaaggactac accctggcag agtcgatgcg
     6481 taaaccgcag tactggatgt tagcggttat gttcctgact gcgtgcatga gtggtctgta
     6541 tgtgattggt gtagcgaaag atatcgctca aagtctggcg catcttgatg caatttccgc
     6601 agccaatgct gtgacggtta tttccatcgc caacctttct ggtcgtctgg tgctgggcat
     6661 tctgtctgac aaaatcgccc gtatccgtgt tatcaccatt ggtcaggtga tatcgctggt
     6721 gggtatggcg gccctgctgt ttgcaccatt gaatgcagtg acgttctttg cagcgattgc
     6781 ctgcgtggcg tttaactttg gcggcactat cacggtgttc ccgtcactgg tcagtgagtt
     6841 cttcggcctc aataacctgg cgaaaaacta cggtgtgatt tatctcggtt tcggtatcgg
     6901 cagcatttgt gggtcgatta tcgcctcact gtttggcggc ttctatgtga ctttctacgt
     6961 catttttgcc ctgctgattc tgtctctggc gctttcaacg accattcgcc agccagagca
     7021 gaaagtattg cgtgaagcgc atggctccct ttaatcatct gccgcgagct actgggcttt
     7081 aaccttagtg tgaatcaaac gcatcaacgc aatgtataaa caatgacgcg taaaatttgg
     7141 gccagttaac tggccctttt tactgctttt tgtaacgcta aagtctcttt tcaaacttgc
     7201 atttttgtaa atttgtgctt catgcacact ctttccccac actttttccc tttgctgtgg
     7261 tctacttatt cgcgcgtgta gattttactt atctgactac ctccgcactt ttcccctgcc
     7321 gggcctgaaa agccgctaag cagggtgtta tcacctgttt gtccagggtt tgtttgcatg
     7381 agatacatca aatcgattac acagcagaag ctgagctttt tgcttgcaat ctatattggc
     7441 ctttttatga atggcgcggt tttttaccgc cgcttcggca gctatgcgca cgattttacc
     7501 gtctggaaag gtatttctgc tgttgttgaa ctggccgcca ccgtactggt gaccttcttt
     7561 ttactgcgtc ttctttcgct gtttggccgc cgcagctggc gtattctggc atcgctggtg
     7621 gtgctctttt ccgcaggtgc cagctattac atgaccttcc ttaatgtagt cattggttat
     7681 ggcatcatcg catccgtcat gaccaccgat atcgacctgt caaaagaagt tgttggtctg
     7741 aactttattc tctggttaat cgccgtaagc gcgttgcctc ttatccttat ctggaataac
     7801 cgctgtcgtt acaccttgct ccgacaactg cgaaccccag ggcagcgtat tcgcagcctg
     7861 gcggtcgtcg tactggcagg tattatggtt tgggcaccga ttcgtttgct ggatatccag
     7921 cagaagaaag tggagagggc gacaggtgtt gatttgccga gttatggcgg cgtcgtcgcg
     7981 aactcttatc tgccatcaaa ctggctttcg gcattaggtc tttatgcctg ggcgcgggtc
     8041 gatgaatctt ccgataacaa ttctctcctt aaccctgcga agaagtttac gtaccaggct
     8101 ccacaaaacg ttgatgatac atatgtcgtt ttcattattg gtgaaaccac gcgctgggat
     8161 catatgggca ttttcggcta tgaacgcaat accacaccga aactggctca ggagaaaaat
     8221 ctggcggcgt tccgtggtta ctcctgtgat actgcaacca aactctcact gcgttgcatg
     8281 tttgtacgtc agggtggagc agaagataat ccgcagcgca cattaaaaga acagaatatt
     8341 ttcgcggtgc tgaagcagtt aggattcact tctgatctct acgctatgca aagcgaaatg
     8401 tggttctaca gcaatacgat ggcggacaac attgcttatc gtgagcagat tggtgcggag
     8461 ccacgtaacc gtggcaaacc ggtagatgat atgttgctgg tagacgaaat gcagcagtcg
     8521 ctggggcgca acccggatgg taagcatctg atcattctgc ataccaaagg ctcacacttt
     8581 aactacaccc agcgttaccc gcgcagcttt gcgcagtgga agccggaatg tattggtgtt
     8641 gatagcggct gtaccaaagc gcagatgatc aactcatacg acaactcggt gacctatgtg
     8701 gatcacttta tttccagcgt gattgatcag gttcgcgata agaaagcgat tgtgttctat
     8761 gcagctgacc acggtgagtc aattaatgaa cgcgagcacc tgcacggcac gccgcgtgaa
     8821 ctggcaccgc cggaacagtt ccgtgtgccg atgatggtct ggatgtcgga taaatatctg
     8881 gaaaatccgg tcaatgcgca ggcatttgcg cagctgaaaa aagaagccga catgaaagtg
     8941 ccacgccgtc atgtagagct gtacgacacc atcatggggt gtcttggcta tacttcaccg
     9001 gatggtggaa ttaacgaaaa caacaactgg tgccacatcc cgcaggcaaa agaggcagcg
     9061 gctaactaag aagcctgctg actttctcgc cgatcaaaag gcattttgct attaagggat
     9121 tgacgagggc gtatctgcgc agtaagatgc gccccgcatt cggtgattgg cgcagcctgg
     9181 tagcgcactt cgttcgggac gaaggggtcg gaggttcgaa tcctctatca ccgaccaaat
     9241 tcgaaaagcc tgctcaacga gcaggctttt ttgcatctgt agctcatgag gaagagaacc
     9301 cccgggggcg gggaggttcg attcgacgta ggcctgataa gacgcgccag cgtcgcatca
     9361 ggcaaatgta ccggcccatt gtcggatgcg gcgcgaccgc cttatccgat ctacacacca
     9421 cacatttccc tgccaaattt gcgccccata tctcactatc cattaacgga tttgtgacag
     9481 aaaccattgt ctttttttat atttgcttaa atcttttttt gcgttactta tggctggcgc
     9541 tggcattttc cgctgtgcgt tttagcgttc atggtattaa tcgctcagat atccaccttt
     9601 acctcagatt tttttcatta aatgcataaa acttaatcac caaatgttgc taaatattaa
     9661 gctgattgtt ctggttgtgt tggcgtagca caaatttctc tgctgcaaat agctgtttga
     9721 agttttttta atctttgttt gtggattctc accgttggtg taaatcccgg ttggctgtat
     9781 tgacgttttc acattctgtt gacagattgt aggtcacgag gggcatttta tggaggatcc
     9841 gcactgttac actgatgtta attagtacgg catccccacc tcataacgtt gacccgaccg
     9901 ggcaaaaaac aaaaaaggtc aggcagcgac aacccactgc aaagggttaa aacaacaaac
     9961 atcacaattg gagcagaata atgcgtattt ccttgaaaaa gtcagggatg ctgaagcttg
    10021 gtctcagcct ggtggctatg accgtcgcag caagtgttca ggctaaaact ctggtttatt
    10081 gctcagaagg atctccggaa gggtttaacc cgcagctgtt tacctccggt accacctatg
    10141 acgcctcttc cgtaccgctt tataaccgcc tggttgaatt taaaatcggc accaccgaag
    10201 tgatcccggg cctcgctgaa aagtgggaag tcagcgaaga cggtaaaacc tataccttcc
    10261 atctgcgtaa aggtgtgaag tggcacgaca ataaagaatt caaaccgacg cgcgaactga
    10321 acgccgatga cgtggtgttc tcgttcgatc gtcagaaaaa cgcgcaaaac ccgtaccata
    10381 aagtttctgg cggcagctac gaatactttg aaggcatggg cttgccggag ctgatcagcg
    10441 aagtgaaaaa ggtggacgat aacaccgttc agtttgtgct gactcgcccg gaagcgccgt
    10501 tcctcgctga cctggcaatg gacttcgcct ctattctgtc aaaagaatat gctgacgcga
    10561 tgatgaaagc cggtacaccg gaaaaactgg atctcaaccc aatcggtacc ggcccgttcc
    10621 agttacagca gtaccagaaa gattcccgta ttcgctataa agcgtttgat ggctactggg
    10681 gcaccaaacc gaagatcgat acgctggtct tctctattac tcctgacgct tccgtgcgtt
    10741 acgcgaaatt gcagaagaac gaatgccagg tgatgccgta cccgaacccg gcagatatcg
    10801 cccgcatgaa gcaggataaa tccatcaacc tgatggaaat gccggggctg aatgtcggtt
    10861 atctctcgta taacgtgcag aaaaaaccac tggatgacgt gaaagttcgc caggctctga
    10921 cctacgcggt gaacaaagac gccatcatca aagcagttta tcagggcgcg ggcgtatcag
    10981 cgaaaaacct gatcccgcca accatgtggg gctataacga cgacgttcag gactacactt
    11041 acgatcctga aaaagcgaaa gccttgctga aagaagcggg tctggaaaaa ggtttctcca
    11101 tcgacttgtg ggcaatgccg gtacaacgtc cgtataaccc gaacgctcgc cgcatggcgg
    11161 agatgattca ggcagactgg gcgaaagtcg gcgtgcaggc caaaatcgtc acctacgaat
    11221 ggggtgagta cctcaagcgt gcgaaagatg gcgagcatca gacggtaatg atgggctgga
    11281 ctggcgataa cggggatccg gataacttct tcgccaccct gttcagctgc gccgcctctg
    11341 aacaaggctc caactactca aaatggtgct acaaaccgtt tgaagatctg attcaaccgg
    11401 cgcgtgctac cgacgaccac aataaacgtg ttgaactgta caaacaagca caggtcgtga
    11461 tgcacgatca ggctccggca ctgatcatcg ctcactccac cgtgtttgaa ccggtacgca
    11521 aagaagttaa aggctatgtg gttgatccat taggcaaaca tcacttcgaa aatgtctcta
    11581 tcgaataatt aaaagccata cccggtggcg ctgtgcctct gcgtgcagcg ccatccggca
    11641 gcaatacttc attccctgtc ccacaagggc gggaattgat ttgtgagcaa tacagacacg
    11701 cagttccagg ctgcgggtca ttatagagaa tccgggttat gttgcagttt attctccgac
    11761 gtttgggact cgtcatcccc acgtttatcg gtattaccct tctcacattt gcctttgtcc
    11821 acatgatccc gggcgatccg gtgatgatca tggcgggcga gcgtgggatc tccccagagc
    11881 gtcacgcgca attgctggct gaactcggct tagataaacc gatgtggcag cagtatctcc
    11941 attacatttg gggcgtaatg cacggtgatt taggcatttc catgaaaagc cgaattccgg
    12001 tatgggaaga gttcgtgccg cgctttcagg ctacgctgga acttggcgtc tgcgcgatga
    12061 tttttgcgac ggcagtcggt attccggttg gtgtgctggc tgcggttaaa cgtggttcca
    12121 ttttcgatca cacagcggtt ggcctggcgc tgaccggcta ctcgatgcca attttctggt
    12181 ggggcatgat gctgatcatg ctggtttcgg tgcactggaa cctgacgcct gtctccggtc
    12241 gtgtgagcga catggtgttc ctcgatgatt ccaatccgtt aaccggtttt atgctgatcg
    12301 acaccgccat ctggggtgaa gacggtaact ttatcgatgc cgtcgcccat atgatcctac
    12361 ccgccattgt gctgggtacc atcccgctgg cggtcattgt gcgtatgacg cgctcctcga
    12421 tgctggaagt gctgggcgaa gattatatcc gcaccgcgcg tgccaaaggt cttacccgga
    12481 tgcgggtgat tatcgtccat gcgctgcgta acgcgatgct gccggtggtg accgttatcg
    12541 gcctgcaggt gggaacattg ctggcggggg cgattctgac tgaaaccatc ttctcgtggc
    12601 ccggtctggg gcgttggttg attgacgcac tgcaacgccg cgattatccg gtagtgcagg
    12661 gcggcgtatt gctggtggcg acgatgatta tcctcgtcaa cctgctggtc gatttgctgt
    12721 acggcgtggt gaacccgcgt attcgtcata agaagtaagg ggacatcatg tcacaggtta
    12781 ctgaaaataa agtgattagc gcaccggtgc cgatgacccc gttacaggag ttctggcact
    12841 attttaaacg caacaaaggc gcggttgtcg ggctggttta cgtcgtcatc gtgctgttta
    12901 tcgcgatctt tgccaactgg attgcaccct ataacccggc ggaacagttc cgcgatgcac
    12961 tgctcgcccc gccagcctgg caggaaggcg gcagcatggc gcacttgctg ggcactgatg
    13021 acgtggggcg tgatgtgctg tcgcggctga tgtacggcgc gcgcttgtcg ctgctggttg
    13081 gctgtctggt agtggtgtta tcgctgatta tgggcgttat tctcggcctg atcgctggtt
    13141 actttggcgg tctggtcgat aacatcatca tgcgcgtggt cgatatcatg ctggcgctgc
    13201 caagtctact gctggcactg gtgctggtgg cgattttcgg cccgtcgatt ggtaacgccg
    13261 cgctggcgct aaccttcgtt gcgctgccgc attatgtgcg cttaacccgc gccgccgtgc
    13321 tggtggaagt taaccgcgat tacgtcaccg cgtctcgcgt ggcgggtgcc ggggcaatgc
    13381 gccagatgtt tattaacatc ttcccgaact gccttgcgcc gctgattgtt caggcgtcgc
    13441 tcggtttctc taacgctatt ctcgatatgg ccgcccttgg cttcctcggc atgggggcgc
    13501 agccgccaac gccggagtgg ggcaccatgc tctctgacgt gttgcagttc gcgcaaagcg
    13561 cctggtgggt cgtgaccttc ccgggtctgg cgatcctgct gacggtgctg gcatttaacc
    13621 tgatgggtga cggtctgcgt gacgcgctcg atcccaaact gaagcagtaa gaggttcgag
    13681 atggcgttat taaatgtaga taaattatcg gtgcatttcg gcgacgaaag cgcaccgttc
    13741 cgcgccgtag accgcatcag ctacagcgta aaacagggcg aagtagtcgg gattgtgggt
    13801 gagtccggct ctggtaaatc ggtcagttcg ctggcgatta tggggctgat tgattatccg
    13861 ggccgcgtaa tggcggaaaa gctggagttt aacggtcagg atttacagcg tatctcagaa
    13921 aaagagcgcc gcaacctggt gggtgccgaa gtggcgatga tcttccagga cccgatgacc
    13981 agccttaacc cgtgctacac cgtgggtttc cagattatgg aagcgattaa ggtgcatcag
    14041 ggcggcaaca aaagtacccg ccgtcagcgg gcgattgacc tgctgaatca ggtcggtatt
    14101 cccgatccgg catcgcgtct ggatgtttac ccgcatcagc tttccggcgg catgagccag
    14161 cgcgtgatga tcgccatggc gattgcctgt cggccaaaac tgctgattgc cgatgaacca
    14221 accactgcgc tggacgtgac cattcaggcg caaatcattg aactactgct ggagctacag
    14281 cagaaagaga acatggcgct ggtgttaatt acccatgacc tggcgctggt ggcggaagcg
    14341 gcacataaaa tcatcgtgat gtatgcaggt caggtggtgg aaacgggcga tgcgcacgcc
    14401 atcttccatg cgccgcgtca cccgtatact caggcattgc tgcgtgcgct gccggaattt
    14461 gctcaggata aagaacgtct ggcgtcgttg ccaggtgttg ttcccggcaa gtatgaccgt
    14521 ccgaacggct gcctgcttaa cccgcgctgt ccctacgcca ctgacaaatg ccgcgctgaa
    14581 gaaccggcgc tgaatatgct cgctgacggg cgtcagtcca aatgccacta cccacttgat
    14641 gatgccggga ggccgacact atgagtacgc aagaggccac ctcgcaacaa ccgctgttgc
    14701 aggctatcga cctgaaaaaa cattatccgg tgaagaaagg tatgttcgcg ccggaacgtc
    14761 tggtgaaggc gctggacggc gtttcgttta accttgaacg tggcaaaacg ctggcagtag
    14821 taggtgaatc tggctgcggt aaatcgaccc tcggtcggtt actgacgatg atcgaaacgc
    14881 ccaccggtgg tgagctgtat taccaggggc aggatctgct caagcacgat ccgcaggcgc
    14941 agaagttgcg tcggcagaaa atccagatcg tctttcagaa tccctatggt tcgctaaatc
    15001 cgcgtaaaaa agttgggcaa attcttgaag agccgctgct tattaatacc agcttaagca
    15061 aagatcagcg tcgggaaaaa gccctgtcga tgatggcgaa agtcggcctg aaaaccgagc
    15121 attacgaccg ctatccgcat atgttctccg gcggtcagcg tcagcgtatc gccattgccc
    15181 gtggtctgat gctcgacccg gatgtggtga ttgccgatga gccggtttcc gcgctggacg
    15241 tctcagtgcg tgcgcaggtg ctgaatctga tgatggattt gcagcaggag ttggggctgt
    15301 cttatgtctt tatctcccac gacctgtcag tggttgagca cattgccgat gaagtgatgg
    15361 tgatgtacct gggccgctgc gtggagaagg gaacgaagga ccaaatcttc aataacccgc
    15421 gtcatccgta cactcaggcg ctactctccg cgacgccgcg cctgaacccg gacgatcgcc
    15481 gcgagcgcat caagctcacc ggtgaactgc caagcccgct caatccaccg ccgggttgcg
    15541 ccttcaacgc ccgctgtcgt cggcgcttcg gcccctgcac ccagttgcag ccgcagctaa
    15601 aagactacgg cggtcaactg gtagcttgtt ttgctgttga tcaggatgaa aatccgcagc
    15661 gttaactcac aaaaccgggg ccgtgcgctc cggtttactc aatcagcttc aacagtggcg
    15721 acaagcatag caaaatgccg taaagcagaa tcagccaggc tttaaatccg cgaagttttt
    15781 ccagttgtgc gactttatag atcaggaaaa acggaatgag gcacgacaca atgccatata
    15841 acgggcttcc caactgaaag aacaccagca ccgatacacg aaacgaaacc caaatcgtca
    15901 acgtaatgac gataaaagcg cagatcgcca gagtcagcac gcgtgaatta attttcttgg
    15961 tatcaataat tcggcttaac agattgagaa taatgccttt aatggcctcg tggaaaccga
    16021 gataaatgcc aaagaatgcg gtcagtacgg caaagatatt cagcaccgta gaggtgatat
    16081 gaatgatatg cccagggatc acctgcgcgg ccagtgccag tgccgagata ttttgttcaa
    16141 aggcagaaac ggcttcttcg tggctaattg agaaggtaaa cgaaaaggca aaaaacagga
    16201 tcaccgcgat gagcgtgaca taactaattc ggtgggtacg cagcgcgagc cgggttgcca
    16261 gtactttatc cgcttcccgt ttacgatagg caatattcat tgggttaagt acctgaataa
    16321 ataccgcaga aaagaagcaa aagggaatgg taagcaggac atcgcggaaa aagacggagg
    16381 cttgcgggaa ggcggttata ttggcgaaat tccagtgcgg gatcatcgca aaaccgaaca
    16441 cgacgataat ccctactttg accaccacca ttggcccgga aatcttaaac agtaatcgtt
    16501 caccaccaga cgcaattgcc accagtacgg cgaaaatggc gactttataa agtagagatt
    16561 gtgaaagatc ggcatcggtt aaaccgaagg ttttcaggta cgaggcgctg tcgaaaacca
    16621 cggagagaga gtagataaaa atcccgtgga taatcatcaa aaagtagata accccgagga
    16681 aaattcccca gttcttcccc aggtaatgac tgataatatc ggtgtagtca ttacaggaat
    16741 cgctttcaga aagggttttt aaataaatgt cctgcactac ccaggtggca ggataagcaa
    16801 tgatcgctgc ggtaataaat acccaaattc ccttcaagcc aatttgtact ggcatcagca
    16861 cggttccggc accaatcgcc atgcctatgc ataataaaac ccagccgaag tcgtagcgtg
    16921 taaacggcaa tttctgatcg tgtttcggta gtgtgttgtg ctgcataaag tcctgtccag
    16981 aaagaagtcg gcctcgcaaa aatgaataaa cccagtttct cttgcgcaac aggtggagaa
    17041 gtcgaatacg cagtatgtaa aaaatgaggc aacagattca ctggggaggt cgcgaggagg
    17101 cgcaattcta caaaattgcg catttcacta ccagtctgga gtcagcccgg atatgaactg
    17161 caactattgc aaataaagaa attaagctgg aatgcatcac gcaatgagga agattgccgg
    17221 gtgctgacgg taaaaagcaa tcgtagaatc agcggcaagc cggaaaggtt ccggtgaggc
    17281 gcaatgttgc gggggcttaa tccctggtgg cattggttgc tggagagaga aaacccccgc
    17341 atattgcagg tatgcacctg acaacatcac gggggctaat cttgaaccta gaccactcaa
    17401 gaatagccgc gaaacgttgt cattacaaca
//
LOCUS       DAMZFT010000105        17203 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.105, whole genome shotgun sequence.
ACCESSION   DAMZFT010000105 DAMZFT010000000
VERSION     DAMZFT010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17203)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17203)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17203
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.105"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(<1..2871)
                     /gene="hrpA"
                     /locus_tag="PN977_003607"
     CDS             complement(<1..2871)
                     /gene="hrpA"
                     /locus_tag="PN977_003607"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415931.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HDI6043607.1"
                     /translation="MTEQQKLTFTALQQRLDSLMLRDRLRFSRRLHGVKKVKNPDAQQ
                     AIFQEMAKEIDQAAGKVLLREAVRPEITYPDNLPVSQKKQDILEAIRDHQVVIVAGET
                     GSGKTTQLPKICMELGRGIKGLIGHTQPRRLAARTVANRIAEELKTEPGGCIGYKVRF
                     SDHVSDNTMVKLMTDGILLAEIQQDRLLMQYDTIIIDEAHERSLNIDFLLGYLKELLP
                     RRPDLKIIITSATIDPERFSRHFNNAPIIEVSGRTYPVEVRYRPIVEEADDTERDQLQ
                     AIFDAVDELSQESPGDILIFMSGEREIRDTADALNKLNLRHTEILPLYARLSNSEQNR
                     VFQSHSGRRIVLATNVAETSLTVPGIKYVIDPGTARISRYSYRTKVQRLPIEPISQAS
                     ANQRKGRCGRVSEGICIRLYSEDDFLSRPEFTDPEILRTNLASVILQMTALGLGDIAA
                     FPFVEAPDKRNIQDGVRLLEELGAITTDEQASAYKLTPLGRQLSQLPVDPRLARMVLE
                     AQKHGCVREAMIITSALSIQDPRERPMDKQQASDEKHRRFHDKESDFLAFVNLWNYLG
                     EQQKALSSNAFRRLCRTDYLNYLRVREWQDIYTQLRQVVKELGIPVNSEPAEYREIHI
                     ALLTGLLSHIGMKDADKQEYTGARNARFSIFPGSGLFKKPPKWVMVAELVETSRLWGR
                     IAARIDPEWVEPVAQHLIKRTYSEPHWERAQGAVMATEKVTVYGLPIVAARKVNYSQI
                     DPALCRELFIRHALVEGDWQTRHAFFRENLKLRAEVEELEHKSRRRDILVDDETLFEF
                     YDQRIRHDVISARHFDSWWKKVSRETPDLLNFEKSMLIKEGAEKISKLDYPNFWHQGN
                     LKLRLSYQFEPGADADGVTVHIPLPLLNQVEESGFEWQIPGLRRELVIALIKSLPKPV
                     RRNFVPAPNYAEAFLGRVKPLELPLLDSLERELRRMTGVTVDR"
     gene            3072..3677
                     /gene="azoR"
                     /locus_tag="PN977_003608"
     CDS             3072..3677
                     /gene="azoR"
                     /locus_tag="PN977_003608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HDI6043608.1"
                     /translation="MSKVLVLKSSILAGYSQSNQLSDYFVEQWREKHSADEITVRDLA
                     ANPIPVLDGELVGALRPSDAPLTPRQQEALALSDELIAELKSHDVIVIAAPMYNFNIS
                     TQLKNYFDLVARAGVTFRYTENGPEGLVTGKKAIVITSRGGIHKDGPTDLVTPYLSTF
                     LGFIGITDVKFVFAEGIAYGPEMAAKAQSDAKAAIDSIVAA"
     gene            complement(3731..5047)
                     /locus_tag="PN977_003609"
     CDS             complement(3731..5047)
                     /locus_tag="PN977_003609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2/dual specificity phosphatase
                     family protein"
                     /protein_id="HDI6043609.1"
                     /translation="MITERRNVLLQGAGWLLLLAPFFFFTYGFLNQFTATQDLNNHDI
                     PSQVFGWETAIPFLPWTILPYWSLDLLYGFSLFVCSSTFEQRRLVHRLILATVMACCG
                     FFLYPLKFSFIRPEVSGVTGWLFSQLELFDLPYNQSPSLHIILCWLLWRHFRQHLAVR
                     WRKVCGGWFLLIAISTLTTWQHHFIDVITGLAVGMLIDWMVPVDRRWNYQKPDQRRIK
                     IALPYVVGACSCIVLMELMIMLQLWWSVWLCWPVLSLLIIGRGYGGLGAITTGKDSQG
                     KLPPAVYWLTLPWRIGMWLSMRWFCLRLEPVSKITAGVYLGAFPRHIPAQNAVLDVTF
                     EFPRGRATKDRLYFCVPMLDLVVPEEGELRQAVAMLETLREEQGGVLVHCALGLSRSA
                     LVVAAWLLCYGHCKTVDEAISYIRARRSRIVLKEEHKAMLKLWENR"
     gene            complement(5037..6794)
                     /locus_tag="PN977_003610"
     CDS             complement(5037..6794)
                     /locus_tag="PN977_003610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional alpha/beta hydrolase/class I
                     SAM-dependent methyltransferase"
                     /protein_id="HDI6043610.1"
                     /translation="MENSRIPGEHFFTTSDNTALFYRHWPTLQPGAKKVIVLFHRGHE
                     HSGRLQHIVDELAMPDTAFYAWDARGHGQTSGPRGYSPSLARSVQDVDEFVRFAASDS
                     QVGLEEVVVIAQSVGAVMVATWVHDYAPAIRGLVLASPAFKVKLYVPLARPALALWHR
                     LRGLFFINSYVKGRYLTHDRQRVASFNNDPLITRAIAVNILLDLYKTSERIVSDAAAI
                     TLPTQLLISGDDYVVHRQPQIDFYQRLRSPLKELHLLPGFYHDTLGEENRAQAFEKMQ
                     SFISRLYANKSQKFDYQHEDRTGPSADRWRLLSGGPVPLSPVDLAYRFMRKAMKLFET
                     HSAGLHLGMSTGFDSGSSLDYVYQNQPQGSNAFGRLVDKIYLNSVGWRGIRQRKTHLQ
                     MLIKQAVADLHAKGLAIRVVDIAAGHGRYVLDALEYEPAVCDILLHDYSELNVAQGQE
                     MIAQRGMSGRVRYEQGDAFNPAELSTLTPRPTLAIVSGLYELFPENEQVKNSLAGLAN
                     AIDPGGILIYTGQPWHPQLELIAGVLTSHKDGKPWVMRVRSQGEMDSLVHDAGFDKCT
                     QRIDEWGIFTVSMAVRRDN"
     gene            complement(6810..7706)
                     /locus_tag="PN977_003611"
     CDS             complement(6810..7706)
                     /locus_tag="PN977_003611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HDI6043611.1"
                     /translation="MLEKSLATLFALLILATLINRFLIWRLPEGKGGEVTLRIRTWWG
                     IVICFSMVISGPRWMTLTFFALISFLALKEYCTLISVHFPRWLYWGIPLNYLLIGFNC
                     FELFLLFIPLAGFLILATWRVLVGDPSGFLHTVSAIFCGWIMTVFALSHAAWLLMLPT
                     TNIQGGALLVLFLLALTESNDIAQYLWGKSCGRRKVVPKVSPGKTLEGLMGGVITTMI
                     ASMIIGPLLTPLNAWQALLAGLLIGVSGFCGDVVMSAIKRDIGVKDSGKLLPGHGGLL
                     DRIDSLIFTAPVFFYFIRYCCY"
     gene            complement(7706..8311)
                     /locus_tag="PN977_003612"
     CDS             complement(7706..8311)
                     /locus_tag="PN977_003612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HDI6043612.1"
                     /translation="MTLYQIKPLFQSLLRPTMFWLNKHHVTANHITLAALALSLLTGL
                     LLMLVAQPILFLLLPIVLFIRMALNALDGMMARECNQQTRLGAILNETGDVISDIALY
                     LPFLFLPESNASLVILMLFCTILTEFCGLLAQTINGIRSYAGPFGKSDRALIFGLWGL
                     AVAIYPQWMQWNNLLWSIASILLLWTAINRCRSVLLMSAER"
     gene            complement(8481..10787)
                     /locus_tag="PN977_003613"
     CDS             complement(8481..10787)
                     /locus_tag="PN977_003613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310036.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2773 domain-containing bactofilin"
                     /protein_id="HDI6043613.1"
                     /translation="MFKPRICSWIGLLPLFMLSLPVQADLRCVANAVDIEPFFSAATA
                     EDKQQVEQAINSSVNLVPFGLSASDWKVHRGDLVVDGNIESNQKLIVLGNLTVKGNIS
                     TFSLSNPWVILGDVTATNIVTDSPLLITGSINASGLVFIDSYYDNPSTIKGSINARGI
                     FINDIIAPVVASSTNSEFMVRASDKNDTENVKKALMIINPDAYYWDLINDEDALKEIF
                     KRSNIRMAGNVCNLMKKEALFRPKPSPELVQELQMLDEGNVAAFEGRDIATFDLAIMR
                     TLPRLKGISANLRKQLINSNDGQTIESMARYMPDNEILELTDQQLGYQPVVLGLLDRE
                     PLSVEIMTRMSHLPDGVGPLNLALRENLPLDIVMTLAKRDWDMIIQELYKDAWLLPES
                     IIDGYIRSNDPTIRQVGAGGQLTYNQAMQLANDSSNDVVTSLASKLAEMKHHGQLLRM
                     TPQESDKIAVYLYQKFENDDDLIGALFLALPDNLQFNFVKRMEKKSPAYFCCRDMQVI
                     HSDAALQRLLTRFNDPEGWSNLAKNQYLSTAMKQKIWHRALSHRKNNPKADSAAYETS
                     ADMILSELISYGEVDDQMLLNAASLIRSEGWDFLESALVSWDNLPAVVLKELQQNMPR
                     NDIWAKFFLRQENSSRAQVNEALRVYYALDPDALAQLDKLAKQPDRIWWSTLAKSNLT
                     FFKFGALNNRHTPSAALAAEIDPEWWIVAMNNPRFPVDVLKARLKRDPLLALELVKPE
                     LDLVRQLALNGKTRAIREQAMRKLDELH"
     gene            complement(10851..11711)
                     /gene="pdxI"
                     /locus_tag="PN977_003614"
     CDS             complement(10851..11711)
                     /gene="pdxI"
                     /locus_tag="PN977_003614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 4-dehydrogenase"
                     /protein_id="HDI6043614.1"
                     /translation="MSSNTFTLGTKSVNRLGYGAMQLAGPGVFGPPRDRHVAITVLRE
                     ALALGVNHIDTSDFYGPHVTNQIIREALYPYSDDLTIVTKIGARRGEDASWLPAFSPA
                     ELQKAVHDNLRNLGLDVLDVVNLRVMMGDGHGPAEGSIEASLTVLAEMQQQGLVKHIG
                     LSNVTPTQVAEARKIAEIVCVQNEYNIAHRADDAMIDALAHDGIAYVPFFPLGGFTPL
                     QSSTLSDVAASLGATPMQVALAWLLQRSPNILLIPGTSSVAHLRENMAAEKLHLSEEV
                     LSTLDGISRE"
     gene            complement(11919..>17203)
                     /locus_tag="PN977_003615"
     CDS             complement(11919..>17203)
                     /locus_tag="PN977_003615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001170212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="autotransporter adhesin BigA"
                     /protein_id="HDI6043615.1"
                     /translation="GGNVTPPKEPKIFNNNVTFDEDKGTLKIRNATFTYSKNTDGTYT
                     LTAGDGRTTVVQGWDVDTAANTVEITGVNTQGGMTWRYGKDGIIYITKTVGATVDDPA
                     SSNVFNLSDAVLTDQGGNAALNGATVIEINGSRIVLNNDGDISATGKDSVVVAMTGND
                     ITVNNNGHMVVDGGTAGVVNGDRAILNNRGDAVITNGGAGVIVTGDNAVINNTGQSDI
                     DGDNSVSVKVAGNATRIKMEGGLNVSGGAHGIDATGDNNEVSNKGNISVVDAHSTGVL
                     LNGDRASFVNMGDINVSGGAADDHAIGVQINGDNSTFINVGDLNADDRATGVKITGDA
                     SDIALAGAMHVGDFASGLEVTGKNNDLSLSTNMMDVTGKQSTGVTITGDENTIDITGD
                     MVVDQNSVGTKIVGDRVSLQQKGDITVNGAGHGVEVSGSKATINNQGKLTVKDQDSIG
                     IAITGDDAQFTTVGEIDVSLNGTGVAISGDREQVNLSGDINIIQERDDSGTFQGGTGI
                     SIMSNDSSMLLAGNINVTSSIGDQPSTSHLSLTGVTIGGENNTVDLQGDVNITVDSGY
                     PGDENSLYGVVVSGSNNIISLDGGINISGDSGGHVVKGVQVTGNNTVNISGHSVMNTR
                     QVLGAFSLISVADGGNVVFDENAITEIQSSVRNTTAPLFVDSSLIVAKGNQSVIQNKG
                     IINTTDAQGLMMANSGAKVVNAGVINIRPDAESHSFFAGMLARGDDSQAKNVSGGTIN
                     LTSITQPSYGGGFSEYPVKWYFNTGYALLASNYGSVINEAGATINLHGAGTYGVSASK
                     GTATNAGEINVDGFVPTVDENGNISDETYWQTNSVYLMGGGMLAGSTDTGNGDAKAVN
                     TGTINVKNEGFGMLAMSGGTVVNQGTINLTADEGVTKQQDNQLFALGAIQRGLAINDQ
                     NGVININTDIGQAFYKDSTGTILNYGKINLFGNPMDESDSHMGVAPDDKDVLSELSGS
                     GESISKTTTADGFIAVNDLANYGDETLNGDVTANGWIFNQPDASLTINGELSVNQGLE
                     NSGHLTTDLLTLNGAVSFFNEGEFSGSITGNSYQQNVVNTGEMTVTEDGHSLVNGSFL
                     FFNEAGATLTNSGNAVTGGENAIIHVTRTGDSVSQVNRGTITAINGYSAIKTENTGSY
                     SNGKWIWNTETGVINGINPAAPLVDLGRGYNFSNAGVINVQGDNAVGISGGITSYTVK
                     LVNSGTINVGTEQGQLDGTNGEGLIGIKGNGKDTTINNTQTGVINVYADNSWAFGGQT
                     KAIINNGEINLLCDTGCDIYAPGTTGTKNDHNGTADITVPEASTTPSQGNVPTPPADS
                     NAPQLLSNYTIGTNSDGSSGTLSANNLVIGDNVSVNAGFSAGTADTTVVVNDVFKGEN
                     ISGVDNIVSSTVVWTAKGSTDASGNVDVTMSKNAYTDVATDGSVSDVAKALDAGYTNN
                     ELYTSLNVGTTAELNSALKQISGSQATTVFREARVLSNRFSMLADAAPKMGNGLAFNV
                     VAKGDPRAELGNNTEYDMLALRKTVDLSESQTMSLEYGIARLDGDGAQKAGDNGVTGG
                     YSQFFGLKHQMSFDNGMNWNNALRYDIHQLDSSRSVAYGDVSKTADTNVKQQYLEFRS
                     EGAKTFEPREGLKITPYAGVKLRHTLEGGYQERNAGDFNLSMNSGSETAVDSIVGLKL
                     DYAGKDGWSANATLEGGPNLSYAKSQRTASLAGAGSQHFNVDDGQKGGSINSLASVGV
                     KYSSKESSLNLDAYHWKEDGVSDKGVMLNFKKTF"
BASE COUNT         4357 a         4632 c         3879 g         4335 t
ORIGIN      
        1 gcggtcaacg gtaacaccgg tcatccgccg taactcgcgc tcaagactgt cgagcaacgg
       61 taactccagc ggtttgacgc ggcctaaaaa cgcttcggca tagtttggcg caggtacaaa
      121 attacggcgt accggtttcg gcaacgattt aatcagagca atcaccagtt cgcggcgcag
      181 gccggggatt tgccattcaa acccgctctc ttctacctga ttaagcagcg gcagcgggat
      241 atgcacagtc acaccgtcag catccgcgcc gggctcaaac tgatagctca aacgcagctt
      301 gagattgccc tgatgccaga agttcggata atccagcttg ctgatttttt ccgccccttc
      361 tttgatcaac atgctctttt caaagttgag caaatcaggc gtttcgcggc tgactttttt
      421 ccaccagcta tcgaagtgac gagcggagat tacatcgtgg cggatgcgct ggtcgtagaa
      481 ctcaaacaac gtttcgtcat caaccagaat atcgcggcga cgtgatttgt gttccagctc
      541 ttccacttcg gcccgcagtt tcaggttttc acggaagaat gcgtgacgcg tctgccagtc
      601 accttccacc agagcgtggc gaataaagag ttcacgacat aacgccggat cgatctggct
      661 gtagttgacc ttgcgcgcag caacaatcgg caaaccataa acagtgactt tttccgttgc
      721 catcaccgcg ccctgcgccc gttcccagtg cggttcgctg taggtgcgtt taatcaaatg
      781 ctgagcaacg ggttctaccc attccgggtc gatacgcgca gcaatgcgcc cccacaggcg
      841 gctggtttct accagttccg ccaccattac ccatttcggc ggctttttaa ataagccgga
      901 accggggaaa atggagaaac gcgcgttacg tgcgccggta tattcttgtt tatcggcatc
      961 tttcatgccg atatgggaaa gcaaaccggt cagtaaggcg atgtgaattt cgcgatactc
     1021 cgccggttcg ctgttaaccg gaatgccaag ttctttcact acctgacgca actgggtgta
     1081 gatatcctgc cattcgcgca cgcgcagata gttgagataa tcggtacgac acaggcgacg
     1141 gaaggcgttg gaagaaagcg ccttttgctg ctcgccaaga taattccaca gatttacaaa
     1201 cgcgaggaag tcggattctt tgtcgtggaa gcgacgatgt ttttcgtccg atgcctgctg
     1261 tttatccatc ggacgctcgc gcggatcctg aatggagagc gcggacgtga taatcatcgc
     1321 ctcacgcacg cagccatgtt tctgcgcttc cagcaccata cgcgccagac gaggatcgac
     1381 aggcaattgc gaaagctggc gaccgagcgg cgtcagttta taggcgctgg cctgttcatc
     1441 cgtggtgatc gcgcccagtt cttcgagcag acgcacgcca tcctggatat tgcgtttatc
     1501 cggtgcttca acaaacggga acgcagcgat atcgcccagc cccagcgcag tcatctgcaa
     1561 aataaccgag gccaggttgg tacgcagaat ctccggatcg gtaaattccg ggcgcgaaag
     1621 gaaatcgtct tcggaataga gacgaataca gatcccttcg gacacacgac cacagcggcc
     1681 tttacgctga ttagctgaag cctgcgaaat gggctcaatc ggcaaacgct gcactttggt
     1741 gcgatagctg tagcggctga tacgcgctgt accggggtcg ataacgtatt taatacccgg
     1801 tacggtcagc gaggtttccg cgacgttggt cgccagcaca atgcgccgtc cactgtgcga
     1861 ctggaacacg cggttctgtt cgctgttcga aagccgcgca taaagcggca agatttcggt
     1921 atgccgcaga tttagcttgt tcagcgcatc ggcggtatcg cgaatttccc gctcaccgct
     1981 cataaagatc agaatgtcgc cagggctttc ctggctcagt tcgtctacgg cgtcaaaaat
     2041 cgcctgcaac tgatcgcgct cggtgtcatc agcttcttca acgatcgggc gatagcgcac
     2101 ttccaccgga taggtccgac cggagacttc gataatcggc gcattattaa agtggcgcga
     2161 aaagcgttcc gggtcgatag tcgcggaagt gataatgatt tttaagtcag gacgccgcgg
     2221 cagcaactct ttcaaatagc cgagcagaaa atcgatattc aggctgcgtt cgtgcgcttc
     2281 gtcaataatg atagtgtcgt actgcatcag caggcggtct tgctggatct ccgccagcag
     2341 gataccgtcg gtcatcagct tgaccatcgt gttatcactt acgtgatcgc tgaaacgcac
     2401 tttatacccg atgcaaccgc ccggctccgt tttcagctct tccgcaatac ggttcgccac
     2461 cgttcgtgcc gccagacgac gcggctgggt atggccgatc agccctttaa tcccgcggcc
     2521 cagttccata cagattttcg gtagctgagt cgttttaccg gaacccgttt ccccggcgac
     2581 gatcaccacc tggtgatcac ggattgcttc gagaatgtcc tgctttttct gactaaccgg
     2641 caaattgtct ggataagtga tttccggtcg tactgcttca cgcagcagga ctttccctgc
     2701 cgcctggtca atctctttcg ccatctcctg gaaaatggcc tgttgtgcat caggattttt
     2761 aaccttcttc acgccgtgca gacggcggga aaaacgcagt ctgtcacgca gcatcagcga
     2821 atccaaccgt tgctgcaagg ccgtaaaggt caatttttgt tgttctgtca taacgttagt
     2881 gggcagtagg ctgcctgatt aattttcttt taaatgatag atgtagagta ccacatcgcg
     2941 gctttctatg cgttgttcaa taaattcgaa cataggcttc gatttattgc gctatctctg
     3001 tggcaggatt gtgaataaag tgtcaacaag caacggggca tcgcccattc aaacatctat
     3061 aaggaaacac catgagcaag gtattagttc ttaaatccag catcctggca gggtactctc
     3121 agtctaatca gttgtccgat tattttgttg aacaatggcg cgaaaagcac tccgctgatg
     3181 aaatcaccgt gcgtgacctg gctgcaaatc cgattccggt actggatggc gaactggttg
     3241 gtgcactgcg tccgagcgat gcgccgctga ctccgcgtca gcaggaagct ctggcgcttt
     3301 ccgatgagct gattgccgag ctgaaatccc acgacgttat cgttattgcg gcaccgatgt
     3361 ataacttcaa catctcgact cagttgaaaa actattttga cctggttgca cgtgcaggcg
     3421 ttactttccg ctataccgag aacggtccgg aaggtctggt aacgggtaaa aaagccatcg
     3481 tgattaccag ccgcggtggg atccataaag atggcccaac ggatctggta acgccgtatc
     3541 tgtccacatt cctgggcttt atcggcatta ccgatgtgaa atttgtcttc gccgaaggga
     3601 tcgcgtacgg cccggaaatg gcagcgaaag cacagtctga cgcgaaagca gccatcgaca
     3661 gtattgttgc tgcataaccg gacactattt tgatgtggac tgtagatatt cagtccacat
     3721 ctcaatccgc ttacctgttt tcccataatt tcagcatcgc tttgtgctct tccttaagca
     3781 caatccgcga gcgtctggct cgaatataac taatcgcttc atcaacggtt ttacaatgtc
     3841 cgtaacataa cagccatgcc gccaccacca gcgcactgcg cgataatccc aacgcgcaat
     3901 ggaccagaac gccgccttgc tcttcgcgta atgtttccag catcgccacg gcctgtcgga
     3961 gctccccctc ttccggaact accagatcca acatcggtac acaaaaatag agtcgatctt
     4021 ttgtcgctcg tccgcgaggg aattcaaagg tgacgtccag aaccgcattc tgtgccggaa
     4081 tatgtcgtgg aaacgcccct aaataaacac cagcagtaat tttgctcacc ggctccaggc
     4141 gaaggcaaaa ccaacgcata gacagccaca tcccgatgcg ccatggcaat gtcagccagt
     4201 aaacggcggg cgggagtttc ccttgactat ctttacctgt tgttatcgcg ccaagcccac
     4261 cgtacccacg gccaataatg agtagcgata atactggcca acataaccag actgaccacc
     4321 ataactgaag cattatcatt agctccatca acacaatgca cgagcacgcg cctacgacat
     4381 atggcagtgc tattttgatt cgacgttgat caggtttctg ataattccaa cgacggtcga
     4441 cgggcaccat ccagtcaatt aacataccta ccgccagccc tgtgatgaca tcaataaaat
     4501 gatgctgcca ggtcgttagc gtcgaaatgg cgatgagtaa aaaccatccg ccacagactt
     4561 tacgccacct cacagccaga tgctgacgaa agtgacgcca aagtagccag cagagaataa
     4621 tatgcagcga aggagactgg ttataaggca gatcaaacaa ttcaagttgc gaaaataacc
     4681 atcctgtcac cccactcact tcaggacgga taaaactaaa cttcagcgga tagagaaaaa
     4741 aaccgcagca ggccattacc gttgccagaa taagccggtg gaccagacgg cgctgctcaa
     4801 atgtcgagct acaaacgaac agcgaaaatc catataaaag atccagactc cagtaaggca
     4861 aaatagtcca gggaagaaaa gggatcgccg tttcccaacc gaatacctga ctggggatat
     4921 catggttgtt aagatcctga gtcgcggtga actgattaag aaatccatat gtgaagaaga
     4981 aaaacggggc cagcaacaat aaccagccag ctccttgtag caatacgttc cggcgttcag
     5041 ttatcacgac gcaccgccat cgaaaccgta aaaatgcccc actcatcaat ccgttgtgtg
     5101 catttatcaa atccggcatc atgcacgagc gaatccatct ccccttgaga acgcacgcgc
     5161 attacccacg gtttaccatc tttatgactg gttaacaccc cggcaatcag ctccagttgt
     5221 gggtgccacg gctgcccggt gtagatgaga atgccacccg gatcgatggc attggcaaga
     5281 cctgcgagtg agttttttac ctgctcattt tcgggaaaaa gctcatacag gccagagaca
     5341 atcgccagcg taggccgcgg agttaacgtg ctgagttctg ccggattaaa cgcatcgccc
     5401 tgttcataac gcacccgccc agacattccc cgttgggcaa tcatctcttg cccctgtgca
     5461 acatttaact cactgtaatc atgtaacaaa atatcgcata cggcaggttc atactccagc
     5521 gcatccagta catagcgccc atgccctgcg gcaatgtcaa ccacgcggat ggctaaacct
     5581 ttggcgtgga gatcggcaac ggcttgttta atcagcattt gtaaatgggt tttgcgctgg
     5641 cgaataccgc gccagccaac actgttcagg tagattttgt cgactaaacg cccgaatgcg
     5701 ttactacctt gcggttgatt ttgatagaca taatccagcg aactgcctga atcaaagccg
     5761 gtgctcattc cgagatgcag gcccgcagag tgcgtctcga acaatttcat cgctttacgc
     5821 ataaagcgat acgccaaatc aaccggcgat aatggcacgg gtccacctga aaggagccgc
     5881 cagcgatccg ctgatggtcc agtgcggtct tcatgctgat aatcaaattt ttgcgatttg
     5941 ttagcatata aacgactaat aaagctttgc attttttcaa atgcctgcgc cctgttctct
     6001 tcacccaacg tgtcgtgata aaagcctggc agcagatgca gctctttcag agggctacgt
     6061 aatctctgat aaaaatcaat ctgcggttga cgatgcacca catagtcatc gcctgatatc
     6121 agaagttgcg tggggagcgt aatcgccgcc gcatcgctaa caatacgttc agacgtttta
     6181 taaagatcga gcaagatatt aacggcaatc gcccgtgtga tcagcggatc attattgaaa
     6241 ctcgccaccc gttgccgatc gtgggtcaaa tagcgtcctt tcacatagga attaataaaa
     6301 aacagaccac gcagacgatg ccataacgcc agcgcaggac gtgccagcgg cacatacaat
     6361 ttaaccttaa aggccggaga agccagcacc agcccgcgaa tagcaggcgc ataatcatga
     6421 acccatgtag caaccatcac tgcgccgacg ctttgcgcga tcacaaccac ctcttccagt
     6481 ccgacctggc tgtcgctggc agcaaaacgg acaaattcat cgacatcctg cactgaacgc
     6541 gcaagagacg gactataacc acgcggcccc gaagtttgtc catgccctcg ggcatcccat
     6601 gcataaaaag cagtatctgg catcgccagt tcatcaacga tatgttgtag acgaccagaa
     6661 tgttcatgac cgcgatgaaa taagacgatg acctttttcg cccctggctg taaagtgggc
     6721 cagtgccgat aaaacaacgc tgtattatca ctggtggtaa aaaaatgttc cccagggatg
     6781 cgtgaatttt ccattttctc tattttcctt cagtaacagc agtagcgaat aaaataaaaa
     6841 aataccggag cggtgaaaat taatgagtca atcctgtcaa ggagtccgcc atgtcctggt
     6901 aatagttttc cactatcttt aacaccaata tctcgtttga tggctgacat cactacatcg
     6961 ccgcaaaaac cacttacccc aatcagtaac cccgccaata atgcctgcca ggcgtttaag
     7021 ggtgtcaata acggtccaat gatcatcgac gctatcatgg tggtgattac gccgcccatc
     7081 agaccttcca gtgtttttcc cgggctgact ttggggacca cttttcttct gccgcaggat
     7141 tttccccata aatactgtgc aatatcgttt gactcggtta acgccaaaag aaataacacc
     7201 agtaacgccc cgccctggat atttgtggtt ggcaacatta ataaccaggc ggcatgactc
     7261 aaggcgaaaa cggtcattat ccagccacaa aaaatggcac tcacggtatg cagaaaacca
     7321 gaggggtcac ccactaaaac tcgccaggtg gctaatatca gaaacccagc caaaggtata
     7381 aataacagaa acagctcaaa gcaattaaaa ccgataagca aatagttaag aggaataccc
     7441 caatataacc aacgcggaaa atgtacggat ataagcgtac aatattcttt caatgccaga
     7501 aaacttatca gcgcaaaaaa cgtcaacgtc atccagcgtg gacctgaaat caccattgaa
     7561 aaacaaatga cgatgcccca ccaggtacga atacgtaatg taacctcacc cccttttccc
     7621 tccggtaacc gccatatcag aaaacggttt atcagcgttg ctaaaattaa caacgcgaaa
     7681 agtgttgcca gagatttttc cagcattatc tttcagcact catcaggagc acacttcgac
     7741 aacgattaat cgcagtccag agaagcaaaa ttgaagcaat gctccataaa agattattcc
     7801 attgcatcca ctgtggataa atggcaacag ccagccccca caacccaaat attaacgcac
     7861 gatcgctttt gccgaatgga ccggcataac tgcgaatacc gttaatcgtc tgcgcgagta
     7921 aaccgcaaaa ctcagttaat atggtgcaaa ataacatgag tattacgagt gatgcgttac
     7981 tttccggtaa aaataaaaac ggtaaataaa gcgcaatatc ggaaataaca tcgccagttt
     8041 cattcaaaat cgccccaaga cgtgtttgct gattgcactc acgcgccatc atgccatcca
     8101 gcgcattgag cgccatacgg ataaaaagca cgataggcaa tagcaaaaag aggatgggtt
     8161 gtgccaccaa catcaacagc aatccggtaa gaagagaaag cgccagtgca gcaagagtga
     8221 tgtgattcgc tgtaacatgg tgcttattaa gccaaaacat cgtcggcctt aacagcgact
     8281 gaaagagcgg ttttatctgg tagagcgtca ttatcaatcc ttgataattc ggtttgcgct
     8341 atggtgcatc aattcccctg acggagatat cgcaacacgc cgcttcatta aaaattcaga
     8401 atatatcaag gtattgggga ctcaccgtca ggaatgatta tccccaatta atcaaatagt
     8461 taaaaatata acgctgttat tcaatgcaac tcatcaagtt ttctcatcgc ttgttcccgt
     8521 attgcgcgtg tcttaccgtt aagcgccagc tggcggacta aatccagttc aggtttaaca
     8581 agctctaacg ccagcaaagg atcacgtttc agcctcgcct ttaatacatc aactggaaaa
     8641 cggggattat tcatcgccac aatccaccac tcgggatcaa tttctgccgc cagtgccgca
     8701 gacggtgtat ggcgattgtt aagtgcgccg aacttgaaaa atgtaagatt gcttttcgcc
     8761 agcgtactcc accatatacg atccggttgt tttgccagtt tatccagttg tgctaaagca
     8821 tcggggtcaa gtgcataata aacacgtaac gcttcgttaa cctgcgcacg agagctgttt
     8881 tcctgcctca gaaaaaactt cgcccaaata tcattgcgtg gcatattttg ctgtaattcc
     8941 ttgaggacaa cagcgggtaa attatcccaa ctaactaatg cactctctaa aaagtcccag
     9001 ccttccgaac gtatcagaga ggcggcattt aatagcatct ggtcatcaac ttcaccgtag
     9061 ctgataagct ccgacaaaat catatcagcg ctggtttcat atgctgcaga atctgctttc
     9121 ggattatttt ttcgatgcga taaagcgcgg tgccaaattt tttgtttcat cgccgtactc
     9181 agatactgat ttttcgccag attactccac ccctcaggat cgttgaaacg tgtcaataac
     9241 cgttgtaaag cagcgtcaga gtgaattacc tgcatatccc gacagcaaaa gtaggcaggg
     9301 gattttttct ccatcctttt aacaaaatta aactgtaagt tatctggcaa cgctaaaaat
     9361 aacgcaccta ttagatcatc gtcattctcg aatttttggt ataaatatac tgctattttg
     9421 tcactctctt gtggcgtcat ccgcaacaat tgaccatggt gtttcatctc tgccagcttt
     9481 gacgctaagc ttgtgacaac atcgtttgat gaatcgttcg ccaactgcat tgcctgattg
     9541 taggtgagtt gtcctccagc accgacctga cgaattgtgg gatcattact gcggatatag
     9601 ccatcaataa tggattcggg caataaccac gcatctttat aaagctcctg aatgatcata
     9661 tcccaatctc ttttcgccag agtcatgacg atatccagag ggagattttc acgtagcgca
     9721 agattcaacg gaccgacacc atcaggtaga tgactcattc gcgtcattat ttcgacagag
     9781 agcggttcac ggtcgagcaa ccccagaaca acaggctggt aacccaattg ctgatcggtc
     9841 aactccagaa tctcattgtc aggcatatat ctcgccatac tttcaatcgt ttgtccatca
     9901 ttgctattaa taagttgttt gcgtaggtta gctgaaattc ctttaagcct tgggagagtt
     9961 cgcattatgg caagatcaaa tgtcgcaata tctcgtcctt caaatgcagc aacgttgcct
    10021 tcatccagca tttgcaattc ctgcactaac tcaggagaag gcttagggcg aaacagcgct
    10081 tcttttttca tcagattaca gacattccct gccatgcgaa tattgcttcg cttaaaaatt
    10141 tctttcagag catcttcatc atttattaga tcccaataat atgcatcagg atttattatc
    10201 atcagcgctt ttttgacatt ttcagtgtca tttttgtcac tcgcacggac catgaattca
    10261 ctatttgtcg aagacgcaac aacaggtgca attatgtcat tgataaatat cccacgcgca
    10321 ttaatactcc ccttaatcgt agacggatta tcgtaatatg agtcgataaa taccagtcca
    10381 cttgcattta tcgaaccggt aattaacagt ggtgaatccg taactatatt cgttgccgtc
    10441 acatcaccga gaataaccca tggattagaa agagagaaag tggaaatatt acctttgact
    10501 gtcaaattgc caagaacaat caatttttga ttactctcta tattaccgtc taccactaaa
    10561 tcgccacgat gcactttcca gtccgatgca gataaaccga aggggacaag attcacgcta
    10621 ctgttgatag cttgttcaac ttgttgttta tcttcagcgg tagccgcaga aaaaaatggt
    10681 tcaatatcaa ctgcatttgc aacacatcga aggtccgcct gtactggaag actcagcata
    10741 aacaggggaa gtaaacctat ccagctgcaa atcctgggct taaacatatg cactccatgc
    10801 cattaaaaaa gaaacaaaag ggaaatgcat ctttcccttt tgtatattcg ttattctcgc
    10861 gaaataccat ccaacgtaga caacacttcc tcagaaagat gcaatttttc agcagccata
    10921 ttctcccgta aatgcgcaac agaagacgtc cctgggatca gcaaaatatt cggtgaacgt
    10981 tgtaacagcc acgccagcgc cacctgcatg ggtgttgcac ccaggctcgc agcaacatcc
    11041 gacagcgtag aggattgcag cggtgtaaag cccccgagcg ggaagaacgg cacgtaggca
    11101 atgccatcgt gggccaaagc atcaatcatt gcatcatcag cacggtgcgc gatgttgtat
    11161 tcgttttgca cacagacaat ttcggcaatc ttgcgcgcct ctgcaacctg cgtcggtgtg
    11221 acgttgctca ggccaatatg ttttaccagg ccttgttgtt gcatctctgc cagcacggtc
    11281 aggctggcct caatcgatcc ttccgctggg ccatgaccat cccccatcat aacgcgcagg
    11341 ttaaccacat ccagtacgtc caggccgaga ttacgtagat tatcgtgcac cgctttttgc
    11401 aactctgccg gagaaaaggc gggcaaccag gaagcgtcct ctccacgccg cgcaccaatt
    11461 ttagtgacaa ttgtcaggtc gtcagagtaa ggataaagcg cttcgcggat aatctgattg
    11521 gtgacgtgcg gaccataaaa gtcgctggta tcaatgtgat tgacgcccaa tgccagcgcc
    11581 tcgcgcagca cagttatagc gacgtggcga tctcttgggg ggccaaaaac cccaggacct
    11641 gccagttgca tcgcgccata accaagacgg ttaacggatt ttgtaccgag agtaaatgta
    11701 ttgctgctca tcgcaccctg ccctgtaata aaacggatta agaaaaatca gtatagaaac
    11761 gaaaagagat aaataatgcg aaggcgtaaa caagattact ggatgagttg ctctattgtc
    11821 ctggaagaca ccaaaacaaa acgcgtcaag cgccgcatcc ggcaataaaa aaccgcaacc
    11881 tgtatcagcg ttgcggttta tgcatcacat actaaaaatt agaacgtttt cttgaaattg
    11941 agcatcacgc ctttatcgct gacgccgtct tctttccagt gataggcatc cagattcagc
    12001 gagctctctt tgctgctgta cttcacgccg acgcttgcca ggctgttgat actgccgccc
    12061 ttctgtccat catcgacatt aaagtgctgg ctgcctgcgc ctgccaggct tgccgtgcgc
    12121 tggctcttcg cgtagctcag gttcggccca ccttccagag tggcgttcgc actccagccg
    12181 tctttgcctg cgtagtccag tttcagcccg acgatgctgt ccaccgccgt ttcgctgcca
    12241 ctgttcatgc tcaggttgaa gtctccggca ttgcgttcct gatagccgcc ctccagcgtg
    12301 tgacgcagtt tcacaccggc atacggggtg attttcagcc cttcgcgcgg ttcaaaggtt
    12361 ttcgcccctt cgctacggaa ctccaggtac tgctgtttca cgttggtatc cgccgttttg
    12421 ctcacgtcgc cgtaagccac cgagcggctg ctgtccagtt gatgaatatc gtaacgcagc
    12481 gcgttattcc agttcatgcc attgtcgaag gacatctgat gtttcagtcc aaagaactgg
    12541 ctgtagccgc cggttactcc gttgtcgcct gctttttgcg caccgtcacc atccagacgc
    12601 gcgataccgt attccaggct catggtctgg ctttcgctca ggtcaactgt tttacgcagc
    12661 gccagcatgt cgtactcggt attgttaccc agttcggcac gcggatcacc tttcgccacc
    12721 acgttgaacg ccagaccgtt gcccatcttc ggcgctgcat ccgccagcat gctgaagcgg
    12781 ttgcttaaca cgcgcgcctc acggaatacg gtggtcgcct ggctaccact gatttgtttc
    12841 agcgcgctgt tcagttcggc ggtggtgccc acgttcagac tggtgtacag ctcgttgttg
    12901 gtataacccg catcaagtgc cttcgccacg tcactcactg agccatcggt cgctacatcg
    12961 gtgtaggcgt ttttgctcat ggtcacgtca acgttgccgc tggcgtcggt actgcctttg
    13021 gcagtccaga cgaccgtaga ggaaacaatg ttatctacac cgctgatgtt ttcgcctttg
    13081 aatacatcgt taacgacaac ggtggtgtcc gctgttccgg cactaaaccc ggcgttaaca
    13141 ctcacgttgt cgccaataac cagattattc gcgctgagcg tacccgaact cccgtcgctg
    13201 ttggtaccga tggtatagtt gctcagcagt tgcggtgcgt ttgaatcggc aggcggcgtt
    13261 ggaacattac cttgtgacgg cgtggttgat gcctctggta cggtgatatc cgcagtaccg
    13321 ttgtgatcgt ttttcgtacc cgtcgttcca ggagcataaa tatcgcatcc ggtgtcacac
    13381 agcaaattaa tttcaccgtt attaatgatg gctttcgttt gcccaccaaa cgcccaggag
    13441 ttatcggcat agacattaat cacacccgtt tgcgtgttat taattgtcgt gtccttacca
    13501 ttgcccttaa taccaattaa gccttcgccg ttggttccat ccagttgccc ctgttcagta
    13561 cccacattaa tggtgccact gttcaccaac ttgacagtat agctggttat gccgccactg
    13621 atgcccaccg cattatcgcc ctgaacatta ataacgccag cgttagaaaa attataacct
    13681 cgtcctaaat caaccaacgg agccgcagga ttaataccat ttatcactcc cgtttccgta
    13741 ttccaaatcc atttcccgtt actgtacgag ccagtatttt cagttttgat tgcgctatag
    13801 ccatttatgg ccgtgatggt gccacggtta acctgcgaaa cagaatcacc ggttctggtt
    13861 acatgaataa tcgcgttttc cccgcccgtt accgcattac cgctatttgt cagggtcgcg
    13921 ccagcttcat tgaagaacag gaaacttccg ttgacaagtg agtggccatc ttcggtcacc
    13981 gtcatttcac cggtattcac cacattctgt tgatagctgt ttccggtaat tgaaccacta
    14041 aattcgcctt cattaaagaa ggaaaccgcg ccattcaacg tcagcagatc ggttgtcagg
    14101 tgaccactgt tttccaaccc ctggtttacg ctcagttcgc cgttgattgt caggctggca
    14161 tcaggttggt tgaatatcca gccgtttgct gtaacgtcgc cattaagcgt ttcgtcgcca
    14221 tagtttgcca ggtcatttac ggcgataaaa ccgtcggcag ttgtggtttt gctgatgctc
    14281 tcgccgctac cagacagctc actgagaaca tctttgtcat ccggcgcaac gcccatatgg
    14341 gaatcacttt catccattgg attaccgaaa agattaattt tgccgtagtt gagaatggtg
    14401 ccggtgctat ctttataaaa cgcctggcca atatcagtgt taatattaat aaccccattc
    14461 tggtcattaa tcgccagacc acgttgaatt gcacccagcg cgaacagttg gttatcttgc
    14521 tgtttcgtca cgccctcatc ggcagtgaga ttaattgtcc cctggtttac aacggtcccg
    14581 ccgctcattg ccagcatgcc gaagccttca ttcttaacgt tgatggtacc ggtattcacc
    14641 gcttttgcat caccgttgcc ggtatccgtt gaaccggcca acatcccgcc gcccatcaga
    14701 tatacggaat tggtctgcca gtaagtttca tcactaatat taccgttttc atctactgtt
    14761 ggtacaaagc cgtcgacatt tatctcgccc gcattggtcg ccgttccttt tgacgcggaa
    14821 acaccatagg ttcctgcccc atgcaaatta atggtcgctc ccgcttcatt aattaccgaa
    14881 ccgtaattac tggccagcaa tgcatagccc gtgttgaaat accatttcac cgggtattca
    14941 ctaaatcctc cgccgtaaga aggctgggta atggatgtca gattgattgt ccctccggag
    15001 acattttttg cctgcgagtc atcgcctctt gccagcattc cagcaaagaa actgtgtgat
    15061 tcagcatctg gcctgatatt aatcaccccg gcattgacaa ccttcgcccc cgaattcgcc
    15121 atcattaatc cctgggcatc ggttgtgtta ataatgcctt tgttctgaat aacagactga
    15181 tttcccttag caacaattaa cgatgaatca acaaatagag gggcagtagt atttctcact
    15241 gaactttgga tctcggttat cgcattttca tcaaatacga cattcccgcc atcagccacc
    15301 gaaatcaaag agaaggctcc caatacctgt cgggtattca ttacggaatg accactaata
    15361 ttgacggtat tattcccggt tacctgcacg cctttgacaa catgtccgcc actatcgccg
    15421 gaaatattga taccaccatc gaggctgatg atgttatttg accccgaaac caccacgccg
    15481 taaagactgt tttcatcccc cgggtaaccc gaatcgacag taatattgac gtcgccttgc
    15541 agatcgacag tattgttttc ccccccgatg gtaacgccgg ttaacgacag gtgtgaggtg
    15601 gacggttggt cccctatgct ggacgtcaca ttaatgtttc ccgccaacag catgctgctg
    15661 tcgttgctca ttatgctgat gcccgtcccg ccctggaaag taccgctgtc gtcacgctcc
    15721 tgaatgatat ttatatcacc gctcaaattc acttgttcac gatcgccgct gatcgcaaca
    15781 cccgtaccat tcagcgagac atcaatctca cccacggtgg taaattgcgc gtcatccccg
    15841 gtgatagcga taccgattga gtcttgatct tttacggtca gtttgccctg attattgatt
    15901 gtcgccttac taccgctgac ttcaacgccg tgccccgcac cgttaacggt aatatcacct
    15961 ttctgttgca acgaaacgcg atcaccaaca atcttcgtcc cgacagagtt ttgatcaacg
    16021 accatatcac cggtgatatc aatggtattt tcatcacctg taatggtcac cccggtggac
    16081 tgtttcccgg tgacatccat catattggtg gacaacgaca ggtcattgtt cttacctgtc
    16141 acctccagcc cggaggcaaa atccccgaca tgcatcgccc cggccagggc aatgtcgctg
    16201 gcgtcaccgg taattttcac cccggtcgcc ctgtcgtcgg catttaaatc tccaacgttg
    16261 ataaaggtac tgttatcgcc gttaatctgc acaccaatag cgtgatcgtc cgctgcacct
    16321 ccgctaacgt taatatcacc catattaacg aatgacgcgc gatcgccgtt cagcagcacg
    16381 cccgtggaat gcgcatccac caccgaaatg ttgcccttgt tgctgacttc gttattgtca
    16441 ccggtggcat caatgccatg cgcgccaccg ctgacgttga gcccgccttc cattttgatt
    16501 ctggttgcgt ttcctgcgac ttttaccgat accgagttgt cgccatcaat atcgctttgc
    16561 cccgtgttgt tgatgaccgc gttgtcgccc gtcacaatga cacccgcgcc cccgttagtg
    16621 atcaccgcat caccacggtt attcaggatc gcacgatcgc cgttaaccac gcccgcggtg
    16681 ccgccatcga caaccatatg gccgttattg ttaacggtaa tgtcgtttcc ggtcatcgcc
    16741 acaaccacag aatcttttcc cgtagcagaa atgtcaccgt cgttattaag gacgatcctg
    16801 ctgccgttaa tttcaatcac cgttgcgcca ttcagcgcgg cattaccgcc ctggtcagtg
    16861 agcaccgcat cgctgaggtt aaatacgttg ctgctcgcgg ggtcatccac cgttgcgccg
    16921 acggttttag tgatatagat aatgccgtct ttaccgtaac gccacgtcat accgccctgg
    16981 gtattcacgc cagtaatttc tacagtattg gcagccgtgt cgacatccca gccttgtaca
    17041 acagtggtcc ggccatctcc agccgtcagg gtataagttc catcggtgtt tttgctgtag
    17101 gtaaaggtgg cgttacgaat tttcagcgtg cctttatctt cgtcgaacgt gacattgtta
    17161 ttgaagattt taggctcttt gggcggggtg acgttgccgc cat
//
LOCUS       DAMZFT010000106        17103 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.106, whole genome shotgun sequence.
ACCESSION   DAMZFT010000106 DAMZFT010000000
VERSION     DAMZFT010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17103)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17103)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17103
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.106"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(<1..968)
                     /gene="trpCF"
                     /locus_tag="PN977_003616"
     CDS             complement(<1..968)
                     /gene="trpCF"
                     /locus_tag="PN977_003616"
                     /EC_number="4.1.1.48"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional indole-3-glycerol-phosphate
                     synthase TrpC/phosphoribosylanthranilate isomerase TrpF"
                     /protein_id="HDI6043616.1"
                     /translation="MMQTVLAKIVADKAIWVETRKEQQPLASFQNEVQPSTRHFYDAL
                     QGARTAFILECKKASPSKGVIRDDFDPARIATVYRNYASAISVLTDEKYFQGSFDFLP
                     IVSQIAPQPILCKDFIIDPYQIYLARYYQADACLLMLSVLDDEQYRQLAAVAHSLEMG
                     VLTEVSNEEELERAIALGAKVVGINNRDLRDLSIDLNRTRELAPKLGHNVTVISESGI
                     NTYAQVRELSHFANGFLIGSALMAHDDLNAAVRRVLLGENKVCGLTRGQDAKAAYDAG
                     AIYGGLIFVATSPRCVNVEQAQEVMAAAPLQYVGVFRNHDIADVVD"
     gene            complement(969..2564)
                     /gene="trpD"
                     /locus_tag="PN977_003617"
     CDS             complement(969..2564)
                     /gene="trpD"
                     /locus_tag="PN977_003617"
                     /EC_number="2.4.2.18"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008784940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional anthranilate synthase glutamate
                     amidotransferase component TrpG/anthranilate
                     phosphoribosyltransferase TrpD"
                     /protein_id="HDI6043617.1"
                     /translation="MADILLLDNIDSFTYNLADQLRSNGHNVVIYRNHIPAQTLIERL
                     ATMSNPVLMLSPGPGVPSEAGCMPELLTRLRGKLPIIGICLGHQAIVEAYGGYVGQAG
                     EILHGKASSIEHDGQAMFAGLTNPLPVARYHSLVGSNIPAGLTINAHFNGMVMAVRHD
                     ADRVCGFQFHPESILTTQGARLLEQTLAWAQQKLEPTNTLQPILEKLYQAQTLSQQES
                     HQLFSAVVRGELKPEQLAAALVSMKIRGEHPNEIAGAATALLENAAPFPRPDYLFADI
                     VGTGGDGSNSINISTASAFVAAACGLKVAKHGNRSVSSKSGSSDLLAAFGINLDMNAD
                     KSRQALDELGVCFLFAPKYHTGFRHAMPVRQQLKTRTLFNVLGPLINPAHPPLALIGV
                     YSPELVLPIAETLRVLGYQRAAVVHSGGMDEVSLHAPTIVAELHDGEIKSYQLTAEDF
                     GLTPYHQEQLAGGTPEENRDILTRLLQGKGDAAHEAAVAANVAMLMRLHGHEDLQANA
                     QTVLEVLRSGSAYDRVTALAARG"
     gene            complement(2564..4126)
                     /gene="trpE"
                     /locus_tag="PN977_003618"
     CDS             complement(2564..4126)
                     /gene="trpE"
                     /locus_tag="PN977_003618"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HDI6043618.1"
                     /translation="MQTQKPTLELLTCKGAYRDNPTALFHQLCGDRPATLLLESADID
                     SKDDLKSLLLVDSALRITALGDTVTIQALSGNGEALLALLDNALPAGVENEQSPNCRV
                     LRFPPVSPLLDEDARLCSLSVFDAFRLLQNLLNVPKEEREAMFFGGLFSYDLVAGFED
                     LPQLAAENNCPDFCFYLAETLMVIDHQKKSTRIQASLFAPNEKEKQRLTARLNELRQQ
                     LTETAPPLPVVSVPHMRCECNQSDEEFGGVVRSLQKAIRAGEIFQVVPSRRFSLPCPS
                     PLAAYYVLKKSNPSPYMFFMQDNDFTLFGASPESSLKYDATSRQIEIYPIAGTRPRGR
                     RADGSLDRDLDSRIELEMRTDHKELSEHLMLVDLARNDLARICTPGSRYVADLTKVDR
                     YSYVMHLVSRVVGELRHDLDALHAYRACMNMGTLSGAPKVRAMQLIAEAEGRRRGSYG
                     GAVGYFTAHGDLDTCIVIRSALVENGIATVQAGAGVVLDSVPQSEADETRNKARAVLR
                     AIATAHHAQETF"
     misc_feature    complement(4148..4241)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00513"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Trp leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00513"
     gene            complement(4218..4262)
                     /gene="trpL"
                     /locus_tag="PN977_003619"
     CDS             complement(4218..4262)
                     /gene="trpL"
                     /locus_tag="PN977_003619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon leader peptide"
                     /protein_id="HDI6043619.1"
                     /translation="MKAIFVLKGWWRTS"
     gene            4400..5281
                     /gene="yciV"
                     /locus_tag="PN977_003620"
     CDS             4400..5281
                     /gene="yciV"
                     /locus_tag="PN977_003620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exoribonuclease"
                     /protein_id="HDI6043620.1"
                     /translation="MSDTNYAVIYDLHSHTTASDGCLTPEALVRRAVEMRVGTLAITD
                     HDTTAAIAPAREEISRSGLALNLIPGVEISTVWENHEIHIVGLNIDITHPLMCEFLAQ
                     QTERRNQRAQLIAERLEKAQIPGALEGAQRLAQGGAVTRGHFARFLVECGKASSMADV
                     FKKYLARGKTGYVPPQWCTIEQAIDVIHHSGGKAVLAHPGRYNLSAKWLKRLVAHFAE
                     HHGDAMEVAQCQQSPNERTQLATLARQHHLWASQGSDFHQPCPWIELGRKLWLPAGVE
                     GVWQLWEQPQNTTEREL"
     gene            5278..5898
                     /locus_tag="PN977_003621"
     CDS             5278..5898
                     /locus_tag="PN977_003621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HDI6043621.1"
                     /translation="MSQFFYIHPDNPQQRLINQAVEIVRKGGVIVYPTDSGYALGCKI
                     EDKNAMERICRIRQLPDGHNFTLMCRDLSELSTYSFVDNVAFRLMKNNTPGNYTFILK
                     GTKEVPRRLLQEKRKTIGMRVPSNPIAQALLEALGEPMLSTSLMLPGSEFTESDPEEI
                     KDRLEKQVDLIIHGGYLGQKPTTVIDLTDDTPVVVREGVGDVKPFL"
     gene            5926..7821
                     /locus_tag="PN977_003622"
     CDS             5926..7821
                     /locus_tag="PN977_003622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
                     /protein_id="HDI6043622.1"
                     /translation="MAGKFRCILLLIVGLFFSSLSYAKNTEIPSYEEGISLFDVEATL
                     QPDGVLDIKENIHFQARNQQIKHGFYRDLPRLWMQPDGDAALLNYHIVGVTRDGIPEP
                     WHLDWHIGLMSIVVGDKQRFLPQGDYHYQIHYQVKNAFLREGDSDLLIWNVTGNHWPF
                     EIYKTLFSLKLPDIAGNPFSEIDLFTGEEGDTYRNGRILEDGRIESRDPFYREDFTVL
                     YRWPHALLGNAPAPQTTNIFSHLLLPSTSSLLIWFPCLFLACGWLYLWKRRPQFTPVD
                     VIETDVIPPDYTPGMLRLDAKLVYDDKGFCADIVNLIVKGKIHLEDQYDKNQQILICV
                     NEGATRNNAVLLPAEQLLLEALFRKGDKVVLTGRRNRVLRRAFLRMQKFYLPRKKSSF
                     YRPDTFLQWGGMAILAVILYGNLSPVGWAGMSLVGDMFIMICWLLPFLFCSLELLFAR
                     DDDKPCVNRVIITLFLPLICSGVAFYSLYINVGDVFFYWYMPAGYFSAVFLTGYLTGM
                     GYIFLPKFTQTGQQRYAHGEAIVNYLARKEAATHSGRRRKGETRKLDYALLGWAVSAN
                     LGKEWAARITPSLTAAVHAPEIARSGVLFSLQMHLSLGANTSLLGRSYSGGGAGGGAG
                     GGGGGGW"
     gene            8032..8907
                     /gene="rluB"
                     /locus_tag="PN977_003623"
     CDS             8032..8907
                     /gene="rluB"
                     /locus_tag="PN977_003623"
                     /EC_number="5.4.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2605) synthase RluB"
                     /protein_id="HDI6043623.1"
                     /translation="MSEKLQKVLARAGHGSRREIESIIEAGRVSVDGKIAKLGDRVEV
                     TPGLKIRIDGHLISVRESAEQICRVLAYYKPEGELCTRNDPEGRPTVFDRLPKLRGAR
                     WIAVGRLDVNTCGLLLFTTDGELANRLMHPSREVEREYAVRVFGQVDDAKLRDLSRGV
                     QLEDGPAAFKTIKFSGGEGINQWYNVTLTEGRNREVRRLWEAVGVQVSRLIRVRYGDI
                     PLPKGLPRGGWTELDLAQTNYLRELVELPPETSSKVAVEKDRRRMKANQIRRAVKRHS
                     QVSGGRRSGGRNNNG"
     gene            9077..10018
                     /locus_tag="PN977_003624"
     CDS             9077..10018
                     /locus_tag="PN977_003624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012602329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6043624.1"
                     /translation="MIRYSGIFLSLLMLSGCATEALKNEQAETVITAGNKAVAANASF
                     FKQYYASRENFLVNFYATNPDCALRPDYTIDIGGDGNGGKLCQRPGPNTKKVQLVPFE
                     KEKLLASMEVINSMSAYVELLTEDMATTESQQVALLKQALDHANNAQTLLGISHSKDL
                     AQSAAAVKDLVNYFNELYIIKVNSGKIGKTVDENWVRLIGNMTVLRNDIYTKSNKIEN
                     YTFANVHDNMFYYNTASKNAKNTEFNTLKNRKEFLSSVINVNETRLQASAPAMPAIEA
                     LNAFIDKGDYLNNLYVNKTRTAAEQKRLNEILRGTGH"
     gene            10090..10398
                     /locus_tag="PN977_003625"
     CDS             10090..10398
                     /locus_tag="PN977_003625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001590091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6043625.1"
                     /translation="MNDVQNRRILALLDMLYNTLGNKTTCNVCHKYFIHILTVMNNLT
                     INYGTETSPELAGINYDDANALITELILFIEDVDAENHETYQRHFNEIIRILNFAIAA
                     "
     gene            complement(10451..11041)
                     /gene="cobO"
                     /locus_tag="PN977_003626"
     CDS             complement(10451..11041)
                     /gene="cobO"
                     /locus_tag="PN977_003626"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HDI6043626.1"
                     /translation="MSDERYQQRQQRVKEKVDARVAQAQDERGIIIVFTGNGKGKTTA
                     AFGTATRAVGHGKKVGVVQFIKGTWPNGERNLLEPHGVEFQVMATGFTWDTQNRESDT
                     AACREVWQHAKRMLADPSLDMVLLDELTYMVAYDYLPLEEVVQALNERPHQQTVIITG
                     RGCHRDILELADTVSELRPIKHAFDAGVKAQIGIDY"
     gene            complement(11038..11796)
                     /locus_tag="PN977_003627"
     CDS             complement(11038..11796)
                     /locus_tag="PN977_003627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciK family oxidoreductase"
                     /protein_id="HDI6043627.1"
                     /translation="MHYQPKQDLLNDRIILVTGASNGIGREAAMTYARYGATVILLGR
                     NEEKLRQVASHINEETGRQPQWFILDLLTCTSEDCQQLAQRIAVNYPRLDGVLHNAGL
                     LGDVCPMSEQNPQVWQDVIQVNVNATFMLTQALLPLLLKSDAGSLVFTSSSVGRQGRA
                     NWGAYAASKFATEGMMQVLADEYQQRLRVNCINPGGTRTAMRASAFPTEDPQKLKTPA
                     DIMPLYLWLMGDDSRRKTGMTFDAQPGRKPGISQ"
     gene            12016..13065
                     /gene="sohB"
                     /locus_tag="PN977_003628"
     CDS             12016..13065
                     /gene="sohB"
                     /locus_tag="PN977_003628"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease SohB"
                     /protein_id="HDI6043628.1"
                     /translation="MELLSEYGLFLAKIVTVVLAIAAIAAIIVNVAQRNKRQRGELRV
                     NNLSEQYKEMKEELAAALMDSHQQKQWHKAQKKKHKQEAKAAKAKAKLGEVATDSKPR
                     VWVLDFKGSMDAHEVNSLREEITAVLAAFKPQDQVVLRLESPGGMVHGYGLAASQLQR
                     LRDKNIPLTVTVDKVAASGGYMMACVADKIVSAPFAIVGSIGVVAQMPNFNRFLKSKD
                     IDIELHTAGQYKRTLTLLGENTEEGREKFREELNETHQLFKDFVKRMRPSLDIEQVAT
                     GEHWYGQQAVEKGLVDEINTSDEVILSLMEGREVVNVRYMQRKRLIDRFTGSAAESAD
                     RLLLRWWQRGQKPLM"
     gene            complement(13101..13352)
                     /locus_tag="PN977_003629"
     CDS             complement(13101..13352)
                     /locus_tag="PN977_003629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciN family protein"
                     /protein_id="HDI6043629.1"
                     /translation="MNKETQPIDRETLLKEANKIIREHEDTLAGIEATGVTQRNGVLV
                     FTGDYFLDEQGLPTAKSTAVFNMFKHLAHVLSEKYHLVD"
     gene            13732..16329
                     /gene="topA"
                     /locus_tag="PN977_003630"
     CDS             13732..16329
                     /gene="topA"
                     /locus_tag="PN977_003630"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000513601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HDI6043630.1"
                     /translation="MGKALVIVESPAKAKTINKYLGSDYVVKSSVGHIRDLPTSGSAA
                     KKSADSTSTKTAKKPKKDERGALVNRMGVDPWHNWEAHYEVLPGKEKVVSELKQLAEK
                     ADHIYLATDLDREGEAIAWHLREVIGGDDARYSRVVFNEITKNAIRQAFNKPGELNID
                     RVNAQQARRFMDRVVGYMVSPLLWKKIARGLSAGRVQSVAVRLVVEREREIKAFVPEE
                     FWEVDASTTTPSGEALALQVTHQNDKPFRPVNREQTQAAVSLLEKARYSVLEREDKPT
                     TSKPGAPFITSTLQQAASTRLGFGVKKTMMMAQRLYEAGYITYMRTDSTNLSQDAVNM
                     VRGYISDNFGKKYLPESPNQYASKENSQEAHEAIRPSDVNVMAESLKDMEADAQKLYQ
                     LIWRQFVACQMTPAKYDSTTLTVGAGDFRLKARGRILRFDGWTKVMPALRKGDEDRIL
                     PAVDKGDALTLVELTPAQHFTKPPARFSEASLVKELEKRGIGRPSTYASIISTIQDRG
                     YVRVENRRFYAEKMGEIVTDRLEENFRELMNYDFTAQMENSLDQVANHEAEWKTVLDH
                     FFSDFTQQLDKAEKDPEEGGMRPNQMVLTSIDCPTCGRKMGIRTASTGVFLGCSGYAL
                     PPKERCKTTINLVPENEVLNVLEGEDAETNALRAKRRCPKCGTAMDSYLIDPKRKLHV
                     CGNNPTCDGYEIEEGEFRIKGYDGPIVECEKCGSEMHLKMGRFGKYMACTNEECKNTR
                     KILRNGEVAPPKEDPVPLPELPCEKSDAYFVLRDGAAGVFLAANTFPKSRETRAPLVE
                     ELYRFRDRLPEKLRYLADAPQQDPEGNKTMVRFSRKTKQQYVSSEKDGKATGWSAFYV
                     DGKWVEGKK"
     gene            16538..>17103
                     /locus_tag="PN977_003631"
     CDS             16538..>17103
                     /locus_tag="PN977_003631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012017585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDI6043631.1"
                     /translation="MKLQQLRYIVEVVNHNLNVSSTAEGLYTSQPGISKQVRMLEDEL
                     GIQIFSRSGKHLTQVTPAGQEIIRIAREVLSKVDAIKSVAGEHTWPDKGSLYIATTHT
                     QARYALPNVIKGFIERYPRVSLHMHQGSPTQIADAVSKGNADFAIATEALHLYEDLVM
                     LPCYHWNRAIVVTPDHPLAGKKAITIEEL"
BASE COUNT         4367 a         4066 c         4443 g         4227 t
ORIGIN      
        1 ttgtccacca catcggcaat atcgtgattg cggaacacgc caacatactg caacggtgcc
       61 gcagccatca cttcctgcgc ctgttcaacg ttgacgcaac gcggtgatgt cgcaacaaaa
      121 atcaacccac cgtaaatcgc gcccgcgtca taagctgctt tagcatcttg cccacgcgtc
      181 aggccacaca ctttattctc acccagcaac acccggcgca cggcggcgtt caaatcgtca
      241 tgggccatca acgccgaacc aatcagaaaa ccgttagcga agtggcttaa ctcgcgcacc
      301 tgagcgtaag tattgatgcc ggattcgctg attaccgtca cgttgtgccc cagtttcggt
      361 gcaagctcgc gggtacggtt gagatcaatc gacaaatcgc gcagatcgcg gttgttgatg
      421 ccaacgacct ttgcccccaa tgcaatggcg cgctccagtt cctcttcatt actgacttcg
      481 gtcagcacac ccatctccag actgtgggcg acggcggcaa gctggcgata ttgttcgtca
      541 tccagtactg aaagcattaa taagcaggca tcagcctggt aatagcgcgc cagatagatc
      601 tggtaagggt cgataataaa gtctttacat aaaatcggct gtggggcgat ttggctgaca
      661 atggggagga aatcaaagct cccctgaaaa tatttctcgt cagtcagcac cgaaattgcc
      721 gaagcgtaat ttctgtagac ggttgcaatg cgtgccggat cgaaatcatc acggatcacg
      781 ccttttgacg gcgacgcttt tttacactcc agaataaacg ccgtgcgtgc gccctgaagt
      841 gcatcataaa aatgtcgcgt gctcggctga acctcattct gaaaactggc cagcggttgc
      901 tgctctttgc gggtttctac ccaaatcgcc ttgtctgcga cgattttcgc taaaacggtt
      961 tgcatcattt accctcgtgc cgccagtgcg gtgactctgt cgtaagcgga accactgcgc
     1021 agtacctcaa gaacggtttg cgcattggct tgcagatctt catggccatg caggcgcatt
     1081 aacatggcga cattcgccgc gacggctgct tcatgggcgg cgtcgccttt accttgtaac
     1141 aagcgtgtta aaatgtcacg gttttcttcc ggtgttccgc ctgccaattg ctcctggtgg
     1201 tagggtgtca ggccaaagtc ttctgcggtg agctgatagc ttttaatttc gccgtcatgc
     1261 agttcggcaa cgattgtcgg cgcgtgtaat gaaacttcat ccatcccgcc gctgtgcacc
     1321 accgccgcgc gttgataccc cagcacgcgc aaggtttcgg caatcggcag caccagctcc
     1381 gggctgtaga caccaattaa cgccagcgga ggatgcgccg ggttaattaa tggccccaga
     1441 acattgaaca gggtgcgggt tttcagttgc tggcgaaccg gcattgcatg gcggaatccg
     1501 gtgtgatatt tcggcgcaaa gaggaaacag acgcctaact catccagcgc ctggcgcgat
     1561 ttatcggcgt tcatatcaag attaataccg aacgccgcca gcagatccga cgagccggat
     1621 ttactggaga cgctacggtt gccgtgtttc gccactttca gcccgcaggc cgcggcgaca
     1681 aacgcactgg cggtagaaat attgatactg ttgctgccgt caccgccagt accgacgata
     1741 tcggcaaaca gataatccgg gcgcgggaac ggcgcggcgt tttccagtag cgcggttgct
     1801 gccccggcga tttcattcgg gtgctcgccg cgaattttca tgctcaccag cgccgccgcc
     1861 agttgttccg gtttcagttc gccacgcacc accgctgaaa atagctggtg gctttcctgc
     1921 tgactaaggg tctgcgcctg atacagtttt tccagaatcg gttgcagcgt gttggttggc
     1981 tctagtttct gctgcgccca ggccagcgtt tgttccagca ggcgagcgcc ctgggtagta
     2041 agaatggatt ccggatggaa ctggaatcca caaacgcgat ccgcatcatg gcgcaccgcc
     2101 atcaccatgc cattaaaatg ggcgttgatg gttaaacctg ccggaatatt actgccaacc
     2161 agcgagtgat aacgcgccac tggcagcggg tttgttaatc cggcaaacat cgcctgaccg
     2221 tcatgttcaa tgctcgacgc tttaccgtga agaatttcgc ccgcctgacc gacatagccc
     2281 ccgtaagctt cgacaatcgc ctgatgaccg aggcaaatgc caataattgg cagcttgccg
     2341 cgcaggcggg tgaggagttc cggcatacaa ccggcttcgc tcggcacacc ggggccagga
     2401 gagagcatca gcaccggatt gctcatcgtc gccaggcgtt caattaaggt ctgcgccgga
     2461 atatggttgc ggtaaatcac cacgttatga ccattgctgc gcaactgatc tgccaggttg
     2521 taagtaaaag aatcgatatt atcgagcagc agaatgtcag ccatcagaag gtctcctgtg
     2581 catgatgcgc ggtggcaata gcgcgcagta cagcgcgggc tttattacgg gtttcgtcgg
     2641 cttccgactg cggaacagaa tcaaggacta cgccagcacc ggcttgcacg gtggcgatac
     2701 cgttttccac cagcgccgag cggatcacaa tgcaggtgtc gagatcgcca tgcgcggtaa
     2761 aataacctac cgcgccgccg tagctgccgc gtcgacgacc ttctgcttcg gcaattaact
     2821 gcatagcgcg tactttcggc gcaccgctta acgtccccat attcatacag gcgcggtaag
     2881 cgtgcagggc gtcgagatcg tggcgcagct caccaacaac gcgggagacc aggtgcatca
     2941 cgtaagagta acggtcaact ttggtgagat cggcaacgta gcggctgccg ggtgtgcaaa
     3001 tgcgagccag gtcattacgg gcgagatcca ccagcatcag atgttcagaa agctctttat
     3061 ggtcggtacg catttcgagt tcgatgcggc tgtcgaggtc tctgtccagt gaaccatcgg
     3121 cacgacgacc gcgtgggcgt gttccggcaa tcgggtagat ctcaatctgg cggctggtgg
     3181 cgtcatactt gagcgaactt tccggcgacg cgccaaacag ggtgaaatca ttatcctgca
     3241 taaaaaacat gtacgggctg ggattactct ttttcagcac gtaataagcc gccagcggtg
     3301 acgggcaggg tagagagaaa cggcgagatg gcaccacctg gaaaatttct ccggcgcgaa
     3361 tcgctttttg caacgaacgc accacgccac cgaactcttc atcgctctga ttacattcac
     3421 aacgcatatg cggcacggaa accaccggca gcggtggcgc ggtttcggtc agttgctggc
     3481 gaagttcgtt caggcgagca gtgagacgtt gtttttcttt ttcattggga gcaaacaggc
     3541 tggcctgaat acgggtgctt tttttctgat ggtcaatcac catcagcgtt tcagcgagat
     3601 aaaaacagaa atcagggcag ttattttccg ctgccagttg cggtaaatct tcaaatcctg
     3661 cgaccaggtc gtaagagaac aggccaccaa agaacatcgc ttcccgttct tccttcggta
     3721 cattcaacag attctgcaat aagcggaaag cgtcaaaaac cgaaagggag cataagcggg
     3781 cgtcttcatc cagcaatgga ctgacaggag ggaagcgcag cacgcggcag tttggtgatt
     3841 gttcattttc cacacccgca ggcagggcgt tatccagtaa tgccaacagg gcttcgccat
     3901 tgccggaaag tgcctgaata gtgacagtgt cacctaatgc tgtaatgcgc agcgcactgt
     3961 ccactagcaa tagacttttt aaatcatctt tgctgtcgat atctgcggat tccagcagca
     4021 gcgttgccgg acgatcccca cacaactggt gaaaaagcgc agtcgggttg tcgcgataag
     4081 cgcctttgca agttagcagt tcgagagtcg gtttttgtgt ttgcattgtt attctctaat
     4141 tttgttcaaa aaaaagcccg ctaattaggc gggctgggta tctgattgct ttacgcatgg
     4201 tgaatacact gcccgtttca ggaagtgcgc caccaacctt tcagtacgaa aattgctttc
     4261 attgtcgata ccctttttac gtgtacttgc gtactagtta actagttcga tgattaattg
     4321 tcaacagctc atttcagaat atttgccaga accgttatga tgtcggcgca aaaaacatta
     4381 tccagaacgg gagtgcgcct tgagcgacac gaattacgca gtgatttacg acctgcacag
     4441 ccataccaca gcttccgatg gctgcctgac gccagaagca ttggtgcgcc gtgcagtcga
     4501 gatgcgcgtc ggcaccctgg cgatcaccga ccatgacacc acagctgcaa ttgcaccagc
     4561 aagagaggaa atttcacgtt cgggactggc gctgaatctt attcccggcg tggaaatttc
     4621 cacggtctgg gaaaatcatg aaattcatat tgtcgggctg aatattgata ttactcatcc
     4681 gctaatgtgt gagttcctcg cgcagcagac agaacggcgc aatcagcggg cgcagctgat
     4741 tgccgaaaga cttgaaaaag cgcaaatacc tggggcactg gaaggcgcgc aacgactggc
     4801 gcagggcggt gcggtgacgc gcggtcattt tgctcgtttc cttgtggagt gcggcaaagc
     4861 cagttcaatg gcggatgtct ttaaaaaata tctggcgcgc gggaaaaccg gatacgttcc
     4921 gccacagtgg tgtacaatag aacaagctat tgatgtcatt catcattctg gcggtaaggc
     4981 ggtgctagct catcctgggc ggtacaatct tagtgctaaa tggctgaaaa gattggttgc
     5041 gcattttgct gaacaccacg gcgacgcgat ggaagtcgcg cagtgccagc aatcgcccaa
     5101 tgaacgcacc cagctggcga cacttgcgcg tcagcatcat ttatgggcat cacaaggatc
     5161 tgattttcat cagccatgcc cgtggatcga actgggtcgt aaactctggc tgcccgcagg
     5221 cgttgaaggc gtctggcagc tatgggaaca gccgcagaac accacagaga gggaattatg
     5281 agccagtttt tttatattca tcctgataac ccgcagcaac gtctgatcaa ccaggcggtg
     5341 gagatcgtgc gtaaaggcgg ggtgattgtt tatccaactg attccggcta tgcgctcggc
     5401 tgtaaaattg aagataaaaa cgccatggag cgtatttgtc gtattcgcca gctgccggac
     5461 ggtcacaact ttaccctgat gtgtcgcgat ctttctgaac tctcgaccta ttcatttgtc
     5521 gataacgttg cgtttcgttt aatgaaaaac aacacgccgg gcaactatac cttcatcctg
     5581 aaggggacga aggaagtgcc acgccgtctg ttgcaggaaa aacgcaaaac catcggtatg
     5641 cgtgtgccgt ctaacccgat tgcgcaggcg ctgcttgaag cattgggcga accgatgctt
     5701 tccacttcgc taatgctgcc aggcagtgaa tttaccgaat ccgatccgga agaaattaaa
     5761 gaccgtctgg aaaaacaggt ggatttgatt attcacggtg gctatctcgg gcagaaaccg
     5821 acaacggtta ttgatcttac cgatgatacg ccggtcgtgg tgcgtgaagg cgtaggtgat
     5881 gtgaagcctt tcttataaca aattgcgttc cacggatgga agactatggc agggaagttt
     5941 cgctgcattt tgctgttgat agttgggctt tttttctctt cgttgagtta tgcgaaaaac
     6001 acggagatcc cttcttatga agaagggatc tctctctttg atgttgaagc cactctgcaa
     6061 ccggatgggg tgctcgacat caaagaaaat attcattttc aggcgcgaaa tcagcagatt
     6121 aagcacggat tttatcgtga tttaccacga ctctggatgc agcctgatgg ggacgctgca
     6181 ctgctgaact atcatattgt tggcgtcacc cgtgatggta ttcctgaacc ctggcatctt
     6241 gactggcata tcgggttaat gagtattgtc gtgggcgata aacaacgttt cttgcctcaa
     6301 ggcgactatc attatcaaat tcattatcag gttaaaaatg ctttcctgcg tgaaggggat
     6361 tctgatctgc taatctggaa cgtgaccggt aaccactggc cgtttgaaat ttataagacc
     6421 ctattttcac tcaagttgcc agatattgcg ggtaatccat ttagcgaaat cgatctcttt
     6481 actggagaag agggcgacac atatcgaaat ggccgcatcc ttgaggacgg aagaattgaa
     6541 tcccgcgatc cgttttatcg tgaagatttc acggtcctct accgctggcc tcacgcttta
     6601 cttggtaatg ccccggcacc acaaacgacg aatattttca gccatcttct tttaccctct
     6661 acgtcatcgt tgttaatttg gtttccgtgt ctcttcctgg cgtgtggatg gttatatctc
     6721 tggaagcgca ggccgcaatt tacgccggta gatgtgattg aaaccgatgt cattccgcca
     6781 gattacacac ccggcatgtt acgtctcgat gcgaagctgg tttacgacga taaaggtttt
     6841 tgtgccgata tcgtaaatct gattgttaaa ggaaaaattc atctggaaga tcagtatgac
     6901 aagaaccagc aaatcctgat ttgtgttaat gaaggcgcga ccagaaataa tgcggtatta
     6961 ctgcccgcag agcagttatt actggaagcg ttatttcgta aaggcgataa ggtcgttctt
     7021 acggggagac gcaacagagt cttacgcagg gcatttttac ggatgcagaa attttatctg
     7081 ccgcgtaaaa agtcttcgtt ttatcgacca gatacgtttt tgcaatgggg cggaatggcg
     7141 atattggcgg tcattctcta cggtaacctg agtcccgtag gttgggcagg aatgagtctg
     7201 gttggcgata tgtttattat gatctgctgg cttcttcctt ttttattttg ttcccttgag
     7261 cttttgtttg cccgcgatga tgacaagcct tgcgttaatc gtgtaatcat cactttgttt
     7321 ttaccgctga tttgttcagg cgtggccttt tattctctct atatcaatgt cggagatgta
     7381 ttcttttact ggtatatgcc agcgggttat tttagcgctg ttttcctgac cggttatctc
     7441 actggcatgg ggtatatttt tctgccaaag tttacccaaa ctgggcagca acgttatgcc
     7501 cacggtgaag ctatcgttaa ctatcttgcg cgtaaagagg cagcaacaca cagtgggcgg
     7561 cggcggaaag gggaaacacg gaaactggat tacgcgttgc taggttgggc agtctcggca
     7621 aaccttggaa aagaatgggc agcacgtatc accccatcac tcacagcggc tgttcacgcc
     7681 ccggaaattg cccgtagtgg cgttttgttt tcattacaga tgcacctgag cctgggggcc
     7741 aataccagtt tgttggggcg aagttattcc ggtggtggtg ctggcggcgg ggcgggtggc
     7801 ggaggcggtg gtggctggta attaagctga tgttaatcga aacaagcttt aaccataaag
     7861 atagtgtagt atgttgcgcc tcaaagccag gccgataaac gtcgagtcgt ttacttaagg
     7921 cctgaagagt tcaaacagac tttactatca gtgagtcaga tgtaaattct gactcactgt
     7981 tgcacaaaag catatcagac gcctgggaag gcgacaccaa aaggaagctc tatgagcgaa
     8041 aagctacaga aagtgctggc gcgtgccggc cacggctctc gccgtgaaat cgaatctatt
     8101 attgaagccg gtcgtgtgag tgttgatggc aaaattgcta aactcggcga tcgcgttgaa
     8161 gttacccctg gattgaaaat ccgtatcgat ggtcacctga tttcggtacg tgagtccgct
     8221 gaacaaattt gtcgcgttct ggcctattac aaaccggaag gtgagttgtg tacccgtaac
     8281 gatccggaag gacgtccaac ggtgtttgac cgtctgccaa aactgcgtgg cgcacgctgg
     8341 attgccgtgg ggcgcctgga cgttaatacc tgtggtctgc tgctgttcac caccgatggt
     8401 gaactggcaa accgtttaat gcacccaagc cgtgaagttg aacgtgaata tgccgtgcgt
     8461 gtatttggtc aggttgacga cgcgaaactg cgtgatttga gtcgtggcgt gcagttggaa
     8521 gatggtccgg cagcctttaa aaccatcaag ttcagcggcg gcgaagggat caaccagtgg
     8581 tacaacgtta ctctgaccga aggccgtaac cgtgaagttc gtcgtctttg ggaagcggtt
     8641 ggtgtgcagg tgagccgtct gatccgcgtg cgttacggtg atatcccact gccgaaaggt
     8701 ctgccacgcg gtggctggac ggagctggac ctcgcccaga ctaactatct gcgtgagctg
     8761 gtggaactac cgccagaaac cagctctaaa gttgctgtag aaaaagaccg tcgtcgtatg
     8821 aaggcgaatc aaattcgtcg tgcggtgaaa cgtcacagcc aggtcagcgg cggtcgtcgt
     8881 tctggtggac gtaataataa cggttaattt aagttaagta taaaataaag gccctgcggg
     8941 gccttttctt catttgaagt atgataaata gtcgcagtat tttaatttaa tttcatactt
     9001 gcttatgaat attaccagga aatagccaca ttactattta attatctgag ataaactcaa
     9061 cgtcaagagg tgggttatga ttagatatag tggaatattt ctatcgctgt taatgctttc
     9121 aggttgtgca accgaagcat tgaagaatga acaagctgaa actgttatta ctgcgggtaa
     9181 caaggcggtt gcggcgaatg caagtttctt taaacaatac tatgcttctc gtgaaaactt
     9241 ccttgttaat ttctatgcaa ctaatccgga ttgtgcactg cgacctgatt ataccataga
     9301 tataggcggt gatggtaatg gcggaaaatt atgccagcgc cctgggccaa atacaaaaaa
     9361 agtacaatta gtccctttcg agaaagaaaa attactggct tcaatggaag tcattaatag
     9421 catgagcgct tatgtagaac ttctgactga ggacatggca acgacagaaa gtcagcaagt
     9481 ggcattactt aaacaggctc tggatcacgc aaacaacgca caaacactac ttggtatttc
     9541 acatagcaaa gatttagcac aatctgcagc agcggttaaa gatttggtaa attatttcaa
     9601 tgagttatat ataataaaag tcaattctgg aaaaataggc aaaacggtag atgaaaactg
     9661 ggtaagactt atagggaaca tgacagtttt gcgtaatgat atttatacaa aaagcaataa
     9721 gattgaaaat tatacctttg cgaatgtgca tgataatatg ttttattata atacggcatc
     9781 taaaaacgct aagaatacag agtttaacac tctgaaaaac aggaaggagt ttctaagttc
     9841 agtaatcaat gtgaatgaaa ccaggctaca ggcatcggca cctgccatgc ccgcaattga
     9901 ggctttaaat gcctttatag ataagggtga ttatctgaat aatttatatg ttaataaaac
     9961 acgcacggca gctgaacaaa aaaggttaaa tgagatattg cgcggcacag gtcattagtg
    10021 ggttaaaggc gtctgagggg ctggcaaaat tacttattgc cagcggaata atgttatagg
    10081 aggcttttta tgaacgatgt gcaaaatcgt agaatactgg cattacttga tatgctgtat
    10141 aataccctgg gcaataaaac cacatgtaat gtttgtcata aatattttat ccatatcctc
    10201 acagttatga acaacctgac gattaactat ggtactgaaa cttcgccaga gttagcaggg
    10261 ataaattatg acgatgctaa tgcgctaatt actgaactga tattatttat tgaagatgtt
    10321 gacgctgaga atcatgaaac atatcagaga catttcaatg agattatacg cattctgaat
    10381 tttgctattg ccgcttgaat ataattatga ttaaagcacc tcaagaggca atcttttgag
    10441 gtgctttccc ttaataatca atccctattt gcgccttaac accggcatca aacgcatgtt
    10501 tgatggggcg cagctcactc accgtatccg ctagttcaag aatatcccga tgacagcctc
    10561 gacccgtgat aatcaccgtc tgttgatgtg gacgttcatt taacgcctgc accacttctt
    10621 ccagtggcaa ataatcatac gccaccatat aagtcagttc atcgagcaaa accatatcca
    10681 gtgaaggatc tgccagcatt cgtttggcat gttgccagac ttcacggcag gcggcggtat
    10741 cagattcgcg attttgcgta tcccaggtaa agcccgttgc catcacctga aattcaacgc
    10801 cgtgtggttc cagcagattt cgttcgccat taggccaggt gcctttaata aactgcacga
    10861 cgcccacttt ttttccgtga ccaactgcgc gtgttgccgt accaaatgct gcggtggttt
    10921 tgccttttcc attgccggta aagacgatga taataccgcg ttcatcctgg gcctgggcca
    10981 cacgggcatc tactttttct ttcactcgct gctgacgctg ttggtagcgt tcatcactca
    11041 ttgggaaatt cctggtttac ggcccggttg ggcgtcaaag gtcatgccgg ttttacggcg
    11101 gctgtcatcg cccatcagcc aaaggtagag cggcatgata tcagcgggtg ttttaagttt
    11161 ctgtggatct tcggtcggga aggcgctggc acgcattgcg gtgcgcgtac cgcctgggtt
    11221 aatgcagttg acgcgcaggc gctgctgata ttcatcggcc agtacctgca tcatcccttc
    11281 ggtggcaaat ttcgacgctg cataagcgcc ccagtttgca cgcccctgac gtccaacgct
    11341 tgatgaagta aagaccagtg aaccggcgtc cgatttgagt aataaaggaa gcagtgcctg
    11401 ggtgagcata aaggtggcat taacgttgac ctgtatgacg tcctgccaga cctgtggatt
    11461 ttgttcgctc attggacaaa catcgccgag caatccggca ttatgcaaaa caccatccag
    11521 acgcggataa ttaacggcaa tgcgctgtgc cagttgttgg caatcttcgg acgtgcaggt
    11581 cagcaaatcg agaataaacc actgtggctg acgcccagtt tcttcgttta tgtggctggc
    11641 tacctgacgt aatttttctt cattacggcc caacagaatc actgtcgcac catagcgtgc
    11701 atacgtcatc gcggcttcac gaccaatacc attgctggct cccgtcacca ggataatgcg
    11761 atcattgagt aaatcttgtt ttggctggta atgcatggct actcctcaac gacgttgtct
    11821 gtcgcgctgc cagtgtactt taagactttg gggctttatg cctgaaacag aagggcattt
    11881 caatcagcct ggggcgataa caccgcaaaa tggatacttt gtcatacttt cgctgcaata
    11941 acatctctgc gagacggctt aacatgcctg ttgtaaactg tgagccaaag cgttgtttaa
    12001 ccaaggtggg gactcgtgga attgttgtct gaatatggtt tgtttttggc gaaaatcgtt
    12061 accgttgtgc tagcgattgc ggcgattgcc gccattatag tcaatgttgc tcaacgtaat
    12121 aaacgccagc gtggcgagtt acgggtcaac aatctcagcg aacagtataa ggagatgaaa
    12181 gaagaactgg ccgcggcgct gatggactca catcagcaaa aacagtggca caaagcgcag
    12241 aagaaaaagc acaagcaaga agcgaaagca gcaaaagcga aagccaaact gggcgaggtg
    12301 gcaactgaca gtaaaccccg cgtctgggtg ctggatttta aaggcagcat ggacgcccat
    12361 gaagtgaact cgctacgtga agagataacg gcggtactcg cagcattcaa accgcaggat
    12421 caggttgtac tccgtctgga aagccctggt ggcatggtgc atggttacgg gttggcggct
    12481 tcgcagctgc agcgtctgcg tgataaaaac attcctttaa ctgttacggt agacaaagtc
    12541 gctgccagcg gcggttacat gatggcctgt gtggcggaca aaattgtttc cgcaccgttt
    12601 gctattgtgg gttccattgg cgtggtggca caaatgccca actttaaccg cttcctgaaa
    12661 agcaaagata ttgatatcga actgcacacc gccgggcagt ataagcgcac gctgaccttg
    12721 ctgggtgaaa acaccgaaga agggcgggag aaattccgcg aagagctgaa cgaaacgcat
    12781 cagttattta aagattttgt gaagcgtatg cgtccgtctc tggatattga acaggtggca
    12841 acgggtgaac actggtacgg acaacaggcg gtagagaaag gcctggttga tgaaatcaac
    12901 accagtgatg aagttattct tagcctgatg gaaggccgtg aagtggtcaa tgtacgctat
    12961 atgcaacgta aacgactcat tgaccgattc accggcagcg cggcagagag cgccgatcga
    13021 ttgttgttac gctggtggca gcgagggcag aagccattga tgtaaaagac aaacgcgagg
    13081 gtaagacctc gcgttttgct ttaatcaacc aggtgatatt tttctgaaag cacatgggcc
    13141 aggtgtttga acatattaaa caccgcggtg cttttggctg ttggcaatcc ttgttcatct
    13201 aaaaagtagt cgccggtaaa taccagcacg ccattacgct gcgtgacgcc ggtggcttca
    13261 atccctgcga gcgtatcctc atgctcacga atgattttgt tggcctcttt caacagcgtt
    13321 tcgcggtcga tgggttgtgt ctctttgttc attatttact ccttaaacaa ggacattagt
    13381 ctacgccagg catggcttgc agacaaatat accacgctgg tggcaagagc gccttactgg
    13441 caactttgga ttttgcatgc taataaagtt gcgtatcgga ttttatcagg tacagtgtga
    13501 cgctttcgtc aatctggcaa tagatttgct tgacattcga ccaaaattcc gtcgtgctat
    13561 agcgcctgta ggccaagacc tgttaactca gtcacctgaa ttttcgtgaa cagagtcacg
    13621 acaaggggtt gatatccgca gagagcgagt ccatatcggt aactcgttgc cagtggaagg
    13681 tttatcaacg tgcgacgcat tcctggaaga atcaaattag gtaaggtgaa tatgggtaaa
    13741 gctcttgtca tcgttgagtc cccggcaaaa gccaaaacga tcaacaagta tctgggtagt
    13801 gactacgtgg tgaaatccag cgtcggtcac atccgcgatt tgccgaccag tggctcagct
    13861 gccaaaaaga gtgccgactc tacctccacc aagacggcta aaaagcctaa aaaggatgaa
    13921 cgtggcgctc tcgtcaaccg tatgggggtt gacccgtggc acaattggga ggcgcactat
    13981 gaagtgttgc ctggtaaaga gaaggtcgtc tctgaactga aacaactggc tgaaaaagcc
    14041 gaccacatct atctcgcaac cgaccttgac cgcgaagggg aagccattgc atggcacctg
    14101 cgggaagtga ttggtggtga tgatgcgcgc tatagccgag tggtgtttaa cgaaattact
    14161 aaaaacgcga tccgccaggc atttaacaaa ccaggtgagc tgaatattga tcgtgttaat
    14221 gcccagcagg cgcgtcgctt tatggaccgc gtggtggggt atatggtttc gccgctgctg
    14281 tggaaaaaga tcgctcgtgg tctgtctgcc ggtcgtgtgc agtcggtggc ggttcgcctg
    14341 gtggtcgagc gtgagcgtga aattaaagcg ttcgtgccgg aagagttctg ggaagtcgat
    14401 gccagcacga ccacgccatc tggtgaagcg ttggcgttac aggtcactca tcagaacgac
    14461 aaaccgttcc gcccggtcaa cagagaacaa actcaggctg cggtaagtct gctggaaaaa
    14521 gcgcgctaca gcgtgctgga acgtgaagac aaaccgacaa ccagtaaacc tggcgctcct
    14581 tttattacct ctacgctgca acaagctgcc agcacccgtc ttggatttgg cgtgaaaaaa
    14641 accatgatga tggcgcagcg tttgtatgaa gcaggctata tcacttacat gcgtaccgac
    14701 tccactaacc tgagtcagga cgcggtaaat atggttcgcg gttatatcag cgataatttt
    14761 ggtaagaaat atctgccgga aagtccgaat cagtacgcca gcaaagaaaa ctcacaggaa
    14821 gcgcacgaag cgattcgtcc ttctgacgtc aatgtgatgg cggaatcgct gaaggatatg
    14881 gaagcagatg cgcagaaact gtaccagtta atctggcgtc agttcgttgc ctgccagatg
    14941 accccagcga aatatgactc cacgacgctg accgttggtg cgggcgattt ccgcctgaaa
    15001 gcacgcggtc gtattttgcg ctttgatggc tggacaaaag tgatgcctgc actgcgtaaa
    15061 ggcgatgaag atcgtatctt accggcagtc gataaaggcg atgctctgac gctcgttgaa
    15121 ctgacaccag cccagcactt taccaagccg ccagcccgtt ttagtgaagc atcgctggtt
    15181 aaagagctgg aaaaacgcgg tatcggtcgt ccgtctacct atgcatcgat catttcgacc
    15241 attcaggatc gtggctatgt gcgagtagaa aatcgtcgtt tctatgcgga aaaaatgggc
    15301 gaaatcgtca ccgatcgcct tgaagaaaat ttccgtgagt taatgaacta cgacttcacc
    15361 gcgcagatgg aaaacagcct cgaccaggtg gcaaatcacg aagcagagtg gaaaactgta
    15421 ctggatcact tcttctcgga tttcactcag cagttagata aagctgaaaa agatccggaa
    15481 gagggtggta tgcgcccgaa ccagatggtt ctgaccagca ttgactgccc gacttgtggt
    15541 cgcaaaatgg ggattcgcac agcgagcacc ggggtattcc ttggctgttc tggttatgcg
    15601 ctgccgccga aagagcgttg caaaaccacc attaacctgg tgccggaaaa cgaagtgctg
    15661 aacgtgctgg aaggcgaaga cgctgaaacc aacgcgctgc gcgcaaaacg tcgttgcccg
    15721 aaatgcggca cggcgatgga cagctatctc atcgatccga aacgtaagtt gcatgtctgt
    15781 ggtaataacc caacctgcga cggttacgag atcgaagagg gcgaattccg cattaaaggt
    15841 tatgacggcc cgatcgttga gtgtgaaaaa tgtggctctg aaatgcacct gaaaatgggg
    15901 cgtttcggta aatacatggc ctgcaccaac gaagagtgta aaaacacacg taagatttta
    15961 cgtaacggcg aagtggcacc accgaaagaa gatccggtgc cattacctga gttgccgtgc
    16021 gaaaaatcag atgcttattt cgtgctgcgt gacggtgctg ccggtgtgtt cctggctgcc
    16081 aacactttcc cgaaatcgcg tgaaacacgt gcgccactgg tggaagagct ttatcgcttc
    16141 cgcgaccgtc tgccggaaaa actgcgttat ctggccgatg cgccacagca ggatccggaa
    16201 ggtaataaga ccatggttcg ctttagccgt aaaaccaaac agcaatatgt ctcttcggaa
    16261 aaagacggaa aggcgactgg ctggtcagca ttttatgttg atggcaaatg ggttgaagga
    16321 aaaaaataac ctttaattct gtcaggtttt tataaacaaa gggtcgcgaa agcggccctt
    16381 ttttattgca tattattttt cttcacacct atacactaag gctataaatg atatagtggt
    16441 tatagttagc ccctttttta ttattaaatc gtattagtca cccgcctggc gtgacgaaaa
    16501 aacgatgttc tgatggcgtc taagtggatg gtttaacatg aaattacaac aacttcgcta
    16561 tattgttgag gtggtcaatc ataacctgaa tgtctcatca acggcggaag gactttacac
    16621 atcacaaccc gggatcagta aacaagtcag aatgctggaa gacgagctag gcattcaaat
    16681 tttttcccga agcggcaagc acctgacgca ggtaacgcca gcaggacaag aaataattcg
    16741 tatcgctcgc gaagtcctgt cgaaagtcga tgccataaaa tcggtcgccg gagagcacac
    16801 ctggccggat aaaggctcgc tgtatatcgc caccacgcat acccaggcac gctacgcttt
    16861 accaaacgtt atcaaaggct ttattgagcg ttatcctcgc gtttctttgc atatgcacca
    16921 gggctcgccg acacaaattg ctgatgccgt ctctaaaggc aatgccgatt tcgcgattgc
    16981 cacagaagcg ctgcatctgt atgaagattt agtgatgtta ccgtgctacc actggaatcg
    17041 ggctattgta gtcactccgg atcacccgct ggcaggcaaa aaagccatta ccattgaaga
    17101 act
//
LOCUS       DAMZFT010000107        16908 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.107, whole genome shotgun sequence.
ACCESSION   DAMZFT010000107 DAMZFT010000000
VERSION     DAMZFT010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16908)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16908)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16908
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.107"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(56..481)
                     /gene="nudI"
                     /locus_tag="PN977_003632"
     CDS             complement(56..481)
                     /gene="nudI"
                     /locus_tag="PN977_003632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphatase NudI"
                     /protein_id="HDI6043632.1"
                     /translation="MRQRTIVCPLIQNDGAYLLCKMADDRGVFPGQWALSGGGVEPGE
                     RIEEALRREIREELGEQLLLTEITPWTFSDDIRTKTYADGRKEEIYMIYLIFDCVSAN
                     REVKINEEFQDYAWVKPEDLVHYDLNVATRKTLRLKGLL"
     gene            759..1301
                     /locus_tag="PN977_003633"
     CDS             759..1301
                     /locus_tag="PN977_003633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6043633.1"
                     /translation="MKKIALAGLAGMLLVSASVNAMSISGQAGKEYTNIGVGFGTEST
                     GLALSGNWTHNDDDGDVAGVGLGLNLPLGPLMATVGGKGVYTNPNYGDEGYAAAVGGG
                     LQWKIGNSFRLFGEYYYSPDSLSSGIQSYEEANAGARYTIMRPVSIEAGYRYLNLSGK
                     DGNRDNAVADGPYVGVNASF"
     gene            1401..2603
                     /locus_tag="PN977_003634"
     CDS             1401..2603
                     /locus_tag="PN977_003634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide deamidase-related
                     protein YfaY"
                     /protein_id="HDI6043634.1"
                     /translation="MLKVEMLSTGDEVLHGQIVDTNAAWLADFFFHQGLPLSRRNTVG
                     DNLDDLVTILRERSQHADVLIVNGGLGPTSDDLSALAAATAKGEGLVLHEAWLKEMER
                     YFHERGRVMAPSNRKQAELPASAEFINNPVGTACGFAVQLNRCLMFFTPGVPSEFKVM
                     VEHEILPRLRERFSLPQPPVCLRLTTFGRSESDLAQSLDSLQLPPGVTMGYRSSMPII
                     ELKLTGPASEEQAMEKLWLDVKRVAGQSVIFEGTEGLPAQISRELQSRQFSLTLSEQF
                     TGGLLALQLSRAGAPLLACEVVPSQEETLAQTAHWITERRANHFAGLALAVSGFENEH
                     LNFALATPDGTFALRVRFSTTRYSQAIRQEVCAMMALNMLRRWLNDQDIASEHGWIEV
                     VESMTLSV"
     gene            2823..3605
                     /locus_tag="PN977_003635"
     CDS             2823..3605
                     /locus_tag="PN977_003635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HDI6043635.1"
                     /translation="MLESSKVPALTRAIDILNLIARIGPCSAATIIDTLGIPKSTAYL
                     LLNELRRQRFLSLDHQENFCLWTRLVELSGHALSKMDLRELARPRLTQLMDTTGLLCH
                     LGIIDNGSAYYILKVESSATISVRSHEGKSLSLYRSGIGKCLLAWQPAAVQQSIIEGL
                     IWEQATPTTITHPQQLHEELARIRRQGWSYDNGEDYADVRCVAAPVFNANNELTAAIS
                     VVGTRLQINEEYRDYLAGKAIACARDISRLLGWKSPFDLQAS"
     gene            3620..4825
                     /gene="rhmD"
                     /locus_tag="PN977_003636"
     CDS             3620..4825
                     /gene="rhmD"
                     /locus_tag="PN977_003636"
                     /EC_number="4.2.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311162.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnonate dehydratase"
                     /protein_id="HDI6043636.1"
                     /translation="MTLPKIKQVRAWFTGGATAEKGAGGGDYHDQGANHWIDDHIATP
                     MSKYRDYEQSRQSFGINVLGTLIVEVEAENGQTGFAVSTAGEMGCFIVEKHLNRFIEG
                     KCVSDIKLIHDQMLNATLYYSGSGGLVMNTISCVDLALWDLFGKVVGLPVYKLLGGAV
                     RDEIQFYATGARPDLAKEMGFIGGKMPTHWGPHDGDAGIRKDAAMVADMREKCGEDFW
                     LMLDCWMSQDVNYATKLAHACAPYNLKWIEECLPPQQYEGYRELKRNAPAGMMVTSGE
                     HHGTLQSFRTLSETGIDIMQPDVGWCGGLTTLVEIAAIAKSRGQLVVPHGSSVYSHHA
                     VITFTNTPFSEFLMTSPDCSTMRPQFDPILLNEPVPVNGRIHKSVLDKPGFGVELNRD
                     CNLKRPYSH"
     gene            4882..6171
                     /locus_tag="PN977_003637"
     CDS             4882..6171
                     /locus_tag="PN977_003637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416749.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDI6043637.1"
                     /translation="MSTALLDAVVKKNRARLIPFMLALYVLAFLDRSNIGFAKQTYQI
                     DTGLSNEAYALGAGIFFVVYAFLGVPANLLMRKLGARTWIGTTTLLWGFLSAAMAWAD
                     TEAKFLIVRTLLGAAEAGFFPGMIYLTSQWFPQRNRASIMGLFYMGAPLALTLGSPLS
                     GALLEMHGFMGHPGWFWMFVIEGLLAVGAGVFTFFWLDDTPEQARFLSKEEKTLLINQ
                     LASEEQQKVTSRLSDALRNGRVWQLAIIYLTIQVAVYGLIFFLPTQVAALLGTKVGFT
                     ASVVTAIPWVAALFGTWLIPRYSDKTGERRNVAALTLLAAGIGIGLSGLLSPVLAIVA
                     LCVAAIGFIAVQPVFWTMPTQLLSGTALAAGIGFVNLFGAVGGFIAPILRVKAETLFS
                     SDAAGLLTLAAVAVIGSLIIFTLRVNRTVAQTDVAHH"
     gene            6189..6992
                     /gene="yfaU"
                     /locus_tag="PN977_003638"
     CDS             6189..6992
                     /gene="yfaU"
                     /locus_tag="PN977_003638"
                     /EC_number="4.1.2.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxy-L-rhamnonate aldolase"
                     /protein_id="HDI6043638.1"
                     /translation="MNALLSNPFKERLRKGEVQIGLWLSSTTSYMAEIAATSGYDWLL
                     IDGEHAPNTIQDLYHQLQAVAPYASQPVIRPVEASKSLIKQVLDIGAQTLLIPMVDTA
                     DQARQVVSATRYPPYGERGVGASVARAARWGRIENYMAHVNDSLCLLVQVESKTALDN
                     LDEILGVEGIDGVFIGPADLSASLGYPDNAGHPEVQRIIETSIRRIRAAGKAAGFLAV
                     APDMAQQCLAWGANFVAVGVDTMLYSDALDQRLAMFKSGKNGPRVKGSY"
     gene            complement(7033..7230)
                     /locus_tag="PN977_003639"
                     /pseudo
     CDS             complement(7033..7230)
                     /locus_tag="PN977_003639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708134.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(7231..8190)
                     /locus_tag="PN977_003640"
     CDS             complement(7231..8190)
                     /locus_tag="PN977_003640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISNCY family transposase"
                     /protein_id="HDI6043639.1"
                     /translation="MTESTTSSPHDAVFKTFMFSPDTARDFLEIHLPEPLRKLCNLQT
                     LRLEPTSFIEKSLRAYYSDVLWSVETSEGDGYIYCVIEHQSSAEKNMAFRLMRYATAA
                     MQRHLDKGYDRVPLVVPLLFYHGETSPYPYSLNWLDEFDDPQLARQLYTEAFPLVDIT
                     IVPDDEIMQHRRIALLELIQKHIRDRDLIGMVDRITTLLVRGFTNDSQLQTLFNYLLQ
                     CGDTSRFTRFIEEIAERSPLQKERLMTIAERLRQEGHQIGWQKGKIEGWQEGKLEGLQ
                     KGKVEGMHEQAIKIALRMLEQGFEREIVLATTQLTDADIPNCY"
     gene            complement(8383..9573)
                     /gene="glpC"
                     /locus_tag="PN977_003641"
     CDS             complement(8383..9573)
                     /gene="glpC"
                     /locus_tag="PN977_003641"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit C"
                     /protein_id="HDI6043640.1"
                     /translation="MNDTSFENCIKCTVCTTACPVSRVNPGYPGPKQAGPDGERLRLK
                     DGALYDEALKYCINCKRCEVACPSDVKIGDIIQRARAKYDTTRPSLRNFVLSHTDLMG
                     SVSTPFAPIVNTATSLKPVRQLLDAALKIDHRRTLPKYSFGTFRRWYRSVAAQQAQYK
                     DQVAFFHGCFVNYNHPQLGKDLIKVLNAMGTGVQLLSKEKCCGVPLIANGFTDKARKQ
                     AITNVESIREAVGVKGIPVIATSSTCTFALRDEYPEVLNVDNKGLRDHIELATRWLWR
                     KLDEGKTLPLKTLPLKVVYHTPCHMEKMGWTLYTLELLRKIPGLELTVLDSQCCGIAG
                     TYGFKKENYPTSQAIGAPLFRQIEESGADLVVTDCETCKWQIEMSTSLRCEHPITLLA
                     QALA"
     gene            complement(9570..10829)
                     /gene="glpB"
                     /locus_tag="PN977_003642"
     CDS             complement(9570..10829)
                     /gene="glpB"
                     /locus_tag="PN977_003642"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase subunit GlpB"
                     /protein_id="HDI6043641.1"
                     /translation="MRFDTVIMGGGLAGLLCGLQLQKHGLRCAIVTRGQSALHFSSGS
                     LDLLSHLPDGQPVTDIHSGLESLRQQAPAHPYTLLGPQRVLDLACQAQALIAESGAQL
                     QGSVELAHQRVTPLGTLRSTWLSAPEVPVWPLPAKKICVVGISGLMDFQAHLAAASLR
                     ELDLAVETAEIELPELDVLRNNATEFRAVNIARFLDNEENWPLLLDALIPVANTCEMI
                     LMPACFGLADDKLWHWLNEKLPCSLMLLPTLPPSVLGIRLQNQLQRQFVRQGGVWMPG
                     DEVKKVTCKNGVVNEIWTRNHADIPLRPRFAVLASGSFFSGGLVAERNGIREPILGLD
                     VLQTATRGEWYKGDFFAPQPWQQFGVTTDEALRPSQAGQTIENLFAIGSVLGGFDPIA
                     QGCGGGVCAVSALHAAQQIAQRAGGQQ"
     gene            complement(10819..12447)
                     /gene="glpA"
                     /locus_tag="PN977_003643"
     CDS             complement(10819..12447)
                     /gene="glpA"
                     /locus_tag="PN977_003643"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit A"
                     /protein_id="HDI6043642.1"
                     /translation="MKTRDSQSSDVIIIGGGATGAGIARDCALRGLRVILVERHDIAT
                     GATGRNHGLLHSGARYAVTDAESARECISENQILKRIARHCVEPTNGLFITLPEDDLS
                     FQATFIRACEEAGISAEAIDPQQARIIEPAVNPALIGAVKVPDGTVDPFRLTAANMLD
                     AKEHGAVILTAHEVTGLIREGATVCGVRVRNHLTGEIQALHAPVVVNAAGIWGQHIAE
                     YADLRIRMFPAKGSLLIMDHRINQHVINRCRKPSDADILVPGDTISLIGTTSLRIDYS
                     EIDDNRVTAEEVDILLREGEKLAPVMAKTRILRAYSGVRPLVASDDDPSGRNVSRGIV
                     LLDHAERDGLDGFITITGGKLMTYRLMAEWATDAVCRKLGNTRPCTTADTPLPGSQEP
                     AEVTLRKVISLPAPLRGSAVYRHGDRTPAWLSEGRLHRSLVCECEAVTAGEVQYAVEN
                     LNVNSLLDLRRRTRVGMGTCQGELCACRAAGLLQRFNVTTSAQSIEQLSTFLNERWKG
                     VQPIAWGDALRESEFTRWVYQGLCGLEKEQKDAL"
     gene            12719..14077
                     /gene="glpT"
                     /locus_tag="PN977_003644"
     CDS             12719..14077
                     /gene="glpT"
                     /locus_tag="PN977_003644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate transporter"
                     /protein_id="HDI6043643.1"
                     /translation="MLSIFKPAPHKARLPAAEIDPTYRRLRWQIFLGIFFGYAAYYLV
                     RKNFALAMPYLVEQGFSRGDLGFALSGISIAYGFSKFIMGSVSDRSNPRVFLPAGLIL
                     AAAVMLFMGFVPWATSSIAVMFVLLFLCGWFQGMGWPPCGRTMVHWWSQKERGGIVSV
                     WNCAHNVGGGIPPLLFLLGMAWFNDWHAALYMPAFCAILVALFAFAMMRDTPQSCGLP
                     PIEEYKNDYPDDYNEKAEQELTAKQIFMQYVLPNKLLWYIAIANVFVYLLRYGILDWS
                     PTYLKEVKHFALDKSSWAYFFYEYAGIPGTLLCGWMSDKVFRGNRGATGVFFMTLVTI
                     ATIVYWMNPAGNPTVDMICMIVIGFLIYGPVMLIGLHALELAPKKAAGTAAGFTGLFG
                     YLGGSVAASAIVGYTVDFFGWDGGFMVMIGGSILAVILLIVVMIGEKRRHQELLQKRN
                     GG"
     gene            14082..15158
                     /gene="glpQ"
                     /locus_tag="PN977_003645"
     CDS             14082..15158
                     /gene="glpQ"
                     /locus_tag="PN977_003645"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HDI6043644.1"
                     /translation="MKLTLKNLSMAIMMSGMIMGSSAMAADSNEKIVIAHRGASGYLP
                     EHTLPAKAMAYAQGADYLEQDLVMTKDDHLVVLHDHYLDRVTDVADRFPDRARKDGRY
                     YAIDFTLDEIKSLKFTEGFDIENGKKVQTYPGRFPMGKSDFRVHTFEEEIEFVQGLNH
                     STGKNIGIYPEIKAPWFHHQEGKDIAAKTLEVLKKYGYTGKDDKVYLQCFDADELKRI
                     KNELEPKMGMDLNLVQLIAYTDWNETQQKQPDGSWVNYNYDWMFKPGAMKQVAEYADG
                     IGPDYHMLIEETSQPGNIKLTGMVQDAQQNKLVVHPYTVRSDKLPEYTTDVNQLYDAL
                     YNKAGVNGLFTDFPDKAVKFLNKE"
     gene            complement(15178..16077)
                     /locus_tag="PN977_003646"
     CDS             complement(15178..16077)
                     /locus_tag="PN977_003646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000176735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDI6043645.1"
                     /translation="MTLTQINSLLAVLDYGGFTEASKRLFMTQSAVSQAIAALEQELG
                     VNILLRDRRKAIQLTSAGHRIMHHLRAINREVNAVKEIAEQEKQNPQRTLRLGCFPSV
                     CACILPAVIRYFEIHHPNIKIIPFEENSTAIIDSLQNESIDAGFVHFPVSGMYSVPIY
                     QDKFTVVLPPNHALAKNSTITVEELMGEPLIISKGRYELSIMALFKEKNITPQIKYEF
                     NHPDTAISFIRQGLGIALLPELTLKTIADELCSVPLEPTFYRQISLLAKEKPVEGSPL
                     FLLQMCTEQLVESGKFDMRQDGA"
     gene            16229..>16908
                     /locus_tag="PN977_003647"
     CDS             16229..>16908
                     /locus_tag="PN977_003647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDI6043646.1"
                     /translation="MKEKLWTKDFWAITIISFIIFFVFYVLLTLLPIYISDRLHASPD
                     KAGLLVTLFLIAAIVIRPFAGQWVGKYSNKTILGLSSLAFLVVTALYPFCHSIESLLF
                     IRVLHGITFGVITTVKGTISARLIPASRRGEGISFFSLAMGLAMVVGPWIGLNMARWE
                     AFNMAFWLCTGVAAVGIILSLIMTVPPVISHADGSTPKMGFAAMFDRAALPFAMVTFF
                     MTFSYAGVS"
BASE COUNT         3845 a         4160 c         4543 g         4360 t
ORIGIN      
        1 aattcgttaa ccactaaatt ttcaatctga cagccagtaa tggctgtcat cattgttaca
       61 gaagaccttt caaacgtaac gtttttcggg tggcgacatt caaatcatag tgcactagat
      121 cttcaggttt tacccacgcg tagtcctgaa actcttcgtt tattttcact tctcggttgg
      181 cagaaacgca gtcaaaaatc aggtaaatca tataaatctc ttccttgcga ccatctgcat
      241 acgtcttggt gcgaatatca tcgctgaagg tccacggcgt gatttctgtc aaaagcagct
      301 gttctcccag ttcttcgcga atttcgcggc gtagtgcctc ttcaattcgt tcgccaggct
      361 ctacgccgcc acccgaaagc gcccattgac cggggaaaac gccgcgatcg tcggccattt
      421 tacacagcaa ataagcacca tcattttgaa tcaaagggca tacaatagtc cgttgtcgca
      481 caggggtatt ccttataaat aaaaagaaac catgagaata cggtaactca gaaatatgtg
      541 aaagtgacag taatctcttt tgcgatgata actgtactta ttattatttt tgtgtgtatc
      601 gataaagtgt gtttattttt gcggcgttta attatttaag aagtatttag ttacaataag
      661 tgactagatc ccttttttcg ctcattgaat cctctgcgcc aggcagtaca atcccgcagc
      721 tttgggaatt ttgattaatt gcttgtggga aaggggatat gaaaaaaatt gcgctagcag
      781 gtctggccgg aatgctgttg gtttctgcat cggtcaatgc aatgagcatc agcggtcagg
      841 cgggtaaaga atacaccaat attggtgtcg gttttgggac tgaatcgacg ggcctggcgt
      901 taagcggtaa ctggacacat aacgacgacg acggtgacgt cgcgggcgtg gggctggggt
      961 tgaatctgcc tctcgggccg ttaatggcga ccgttggcgg aaaaggcgtt tacaccaacc
     1021 cgaattacgg cgatgaaggt tatgccgcag cggtaggagg tggtttgcag tggaaaattg
     1081 gcaacagttt ccgtctgttt ggcgagtatt attactcccc ggattcgctc tccagcggta
     1141 ttcaaagtta tgaggaagcg aatgcgggcg cgcgttacac cattatgcgt ccagtcagta
     1201 ttgaggcggg ctatcgctac ctgaaccttt ctggtaaaga cggtaaccgc gacaacgccg
     1261 tggctgacgg cccgtatgtt ggggttaacg ccagtttctg atcccttccg gcgcggcatt
     1321 ctgccgcgcc attcagtttc ttactacgcc ttatctccct ttgcgttata gtgattcgat
     1381 aacaaaagca ggagataacc atgttaaaag tggaaatgtt atccaccggg gatgaagtgt
     1441 tacacgggca aatcgttgac actaacgctg cctggctggc cgattttttc tttcatcagg
     1501 ggttgccatt atctcgccgc aatacggtgg gggataacct tgatgactta gtcaccattc
     1561 ttcgcgaacg gagtcagcac gccgatgtgc tgatcgttaa cggcgggctg gggccgacca
     1621 gtgatgattt aagcgcactc gccgctgcga cagcaaaagg tgaaggcctg gtgttgcatg
     1681 aagcctggct caaagagatg gaacgctatt tccacgaacg tggacgagta atggcaccga
     1741 gcaaccgtaa acaagcggag ctacctgcca gtgctgaatt tatcaataac ccggtaggca
     1801 ccgcctgtgg ttttgccgtg cagctcaatc gttgcctgat gttctttacc cccggcgtac
     1861 cgtcagaatt taaggtgatg gtcgagcacg aaatcctgcc gcgcctgcgc gagcgttttt
     1921 ctttaccgca gccgccggtt tgtctgcgtt taactacctt tggtcgttcg gaaagcgatc
     1981 tggcgcaaag cctggactcc ctgcaactgc cgccgggcgt aacaatgggc tatcgctcct
     2041 caatgccgat tatcgaactg aaactcaccg gaccggcaag cgaggaacag gcgatggaaa
     2101 aactgtggct ggacgttaaa cgtgttgccg gacagagcgt gattttcgaa ggcaccgaag
     2161 gactgcccgc gcagatcagt cgcgaattgc agagccgcca gttcagcctg acgttgagcg
     2221 agcaattcac cggtggttta ttggctttgc aactttctcg cgcaggtgct ccattgctgg
     2281 cgtgtgaagt ggttccttca caggaagaaa ccctggcgca aactgcgcac tggattacag
     2341 aacggcgggc caaccatttt gccgggctgg cactggctgt ttcgggtttc gagaacgagc
     2401 atctcaactt tgcgctagcc acgccagacg gcactttcgc tctgcgtgtg cgtttcagca
     2461 ctacgcgcta cagccaggct atccgtcagg aagtgtgcgc aatgatggca ctgaatatgc
     2521 tgcgccgttg gttaaacgac caggacatcg ccagtgagca tggctggatt gaggttgttg
     2581 agtccatgac cttatctgtc tgattccgcc actctgtgcc ggtttactgc accggcacat
     2641 tccccctccg gcaaaatttg tccaacctgt tgtactcaat aagaaaaaat ccgtgcgcta
     2701 attcacaaat ttgtgcgtaa tttctccagt ccgctagacg aggagcggga tttgatgcgc
     2761 ttcacaattt tactccccct gagcaacgag actgattcca gtatctaaga ctggagtcca
     2821 gtatgttgga atcaagcaaa gttcccgccc tgacacgggc tattgatatt ctcaatttga
     2881 ttgcccgcat tggcccctgt agtgctgcga cgatcattga cacattgggg atccctaaaa
     2941 gcacggccta tttgctgctt aatgaactca ggcgtcagcg ttttcttagc cttgatcacc
     3001 aggaaaattt ttgtttgtgg accaggctgg tggagctttc cggtcatgcg ttaagcaaga
     3061 tggatctccg ggaactggct cgaccgcgtt tgacgcaact gatggatacc accgggctgt
     3121 tatgtcacct ggggattatt gataacggca gcgcttatta cattttgaaa gtggagtcat
     3181 cagccactat cagcgtgcgt tcccatgaag gaaaaagcct ttcgctttat cgctccggaa
     3241 taggtaaatg cctgcttgcc tggcaacctg cagctgtaca gcaaagtatt atcgaagggc
     3301 tgatctggga gcaggcgaca cccacaacga ttactcatcc acagcaactt cacgaagaac
     3361 tggcgcgtat tcgccgtcag ggctggagct atgacaacgg cgaagattac gccgatgtcc
     3421 gttgtgtcgc tgcgccggtt tttaacgcca ataacgagct taccgctgcg atctcggtgg
     3481 tcggtacccg cttacaaatc aatgaagaat accgtgatta tctggcgggc aaggccattg
     3541 cctgcgccag ggatatttca cgtctgttgg gatggaaaag tccctttgac ttacaggcct
     3601 cataacaatg gagaacagca tgaccctacc aaaaatcaaa caggttcgcg cctggtttac
     3661 tggcggtgcg acagcagaaa aaggcgctgg cggtggtgat tatcacgacc agggggcgaa
     3721 tcactggatt gacgatcata ttgccacccc gatgagtaaa taccgcgatt acgagcagtc
     3781 acgccagtca tttggcatta acgttcttgg cacgttgatt gttgaagtcg aagcagaaaa
     3841 cggccagacc ggattcgccg tttcgacagc cggtgaaatg ggctgtttta ttgtcgaaaa
     3901 acaccttaac cgtttcattg agggtaaatg tgtcagtgat atcaaactga tccacgatca
     3961 aatgctcaat gccaccctgt attactccgg ctctggtggc ctggtgatga atacgatttc
     4021 ctgtgtcgat ctggctctgt gggatctgtt cggcaaagtg gtcgggcttc cggtttataa
     4081 acttttaggc ggcgcagttc gtgatgagat tcagttctac gccacaggtg cgcgtccgga
     4141 tctggcaaaa gagatgggct ttatcggtgg caaaatgccg acgcactggg ggccacatga
     4201 tggcgatgcg gggatccgca aagatgccgc tatggtcgcg gatatgcgtg aaaaatgcgg
     4261 tgaggatttc tggttaatgc tcgactgctg gatgagtcag gacgtgaact atgccaccaa
     4321 actggcccac gcttgcgcgc cctataacct gaaatggatc gaagagtgcc tgccgccaca
     4381 gcagtatgaa ggttatcgcg aactgaaacg caacgcacca gcggggatga tggtcaccag
     4441 cggtgagcac cacggcacat tgcaatcttt ccgtacgctt tcagaaaccg gtatcgacat
     4501 tatgcagccg gatgttggct ggtgcggtgg cttaaccacg ctggtggaaa ttgccgcaat
     4561 cgccaaatcc agggggcaac tggtggtgcc gcacggttcg tctgtttact cccaccatgc
     4621 ggtgatcacc ttcaccaata cgccattcag tgaattcctg atgaccagcc cggattgttc
     4681 aacgatgcgt ccgcaatttg atccgattct gcttaatgag ccggttccgg tgaatggtcg
     4741 tattcataaa tcagtgcttg ataaacccgg tttcggcgtt gaactcaatc gtgactgcaa
     4801 tttgaaacgc ccctacagcc actaatcact ccgggcgttg ctgtgacaac ggtaacgccc
     4861 acatattcta aggagctttt tatgagcacc gctttgcttg acgccgtggt gaagaaaaac
     4921 cgcgcgcgtt taattccgtt tatgttggcg ctgtatgtgc tggcgtttct cgaccgttcg
     4981 aatattggtt ttgccaaaca gacctaccag attgataccg ggctgagtaa tgaagcttat
     5041 gcgctgggag caggcatttt ctttgtggta tacgcgtttc tgggggttcc ggcgaatctt
     5101 ttgatgcgca aactgggggc aagaacctgg attggtacga caacactgct gtggggattt
     5161 ctttcggctg ccatggcatg ggccgatact gaagcgaaat ttctgattgt tcgcactctg
     5221 cttggtgctg cggaggctgg gtttttccct ggtatgattt atctcacctc gcaatggttt
     5281 ccgcagcgta atcgcgccag cattatgggg ctgttctata tgggcgcacc gctggcgtta
     5341 acactgggat caccgctttc tggcgcgctg ctggagatgc atggatttat ggggcatccc
     5401 ggctggttct ggatgtttgt tattgaagga ttgttggcag tcggcgctgg ggtattcaca
     5461 ttcttttggc ttgatgacac accggagcag gcacgttttc tgagtaaaga agaaaaaacg
     5521 ttgcttatca accaactggc aagtgaagaa caacagaaag tgacttcccg actgagcgat
     5581 gcgctgcgta atgggcgagt ctggcaactg gcgattatct acctgaccat tcaggtagcg
     5641 gtttacggat taattttctt cctgccgacc caggttgctg cattgctggg gacaaaagtg
     5701 ggctttacgg cgtcggtggt caccgccatt ccgtgggttg cggccttgtt tggaacctgg
     5761 cttattccgc gctattccga taaaaccggc gaacggcgta atgtcgcagc gctgacatta
     5821 ctggcggcgg gcattggtat tggtctgtcc gggctgcttt ctccagtact ggcgatcgta
     5881 gcgctgtgtg ttgcagccat cgggtttatt gccgtgcagc cggtgttctg gacgatgccg
     5941 acacaactgc tttccggtac ggcgctggct gcggggattg gttttgtaaa cctgtttggt
     6001 gcagtgggcg ggtttattgc cccgatcctg cgcgtgaaag cagaaacgtt attttccagc
     6061 gatgcggcgg gattactgac gctggcagcg gtggcggtca tcggttcgct gattattttc
     6121 actctgcgtg taaatcgcac tgttgcgcag accgacgtgg cacatcatta aataggttaa
     6181 ggaacacgat gaacgcatta ttaagcaatc cctttaaaga acgtttacgc aagggcgaag
     6241 tgcaaattgg tctgtggtta agctcgacta cttcttatat ggcagaaatt gccgccacct
     6301 ccggttatga ctggttgctg attgacgggg agcacgcgcc aaacaccatt caggatcttt
     6361 atcaccagct acaggcggta gcgccctatg ccagccaacc cgtgatccgt ccggtggaag
     6421 ccagtaagtc gctgattaaa caagtcctgg atattggcgc gcaaactcta ctgatcccga
     6481 tggtcgatac tgccgatcag gcacgtcagg tggtgtctgc cacgcgctat cctccctacg
     6541 gtgagcgtgg tgttggggcc agtgtggcac gggctgctcg ctggggacgc attgagaatt
     6601 acatggcgca cgtcaacgat tcgctttgtc tgttggtgca ggtggaaagt aagacggcac
     6661 tggataacct ggacgaaatc ctcggcgtcg aaggaattga tggcgtgttt attggacctg
     6721 cggatctttc tgcctcgttg ggctacccgg ataacgccgg gcacccggaa gtgcagcgaa
     6781 ttattgaaac cagcattcgg cggatccgtg ctgcgggtaa agctgctggt tttctggctg
     6841 tggctcctga tatggcgcag caatgcctgg cgtggggagc gaactttgtc gctgttggcg
     6901 ttgacacgat gctctacagc gatgccctgg atcaacgact ggcgatgttt aaatcaggca
     6961 aaaatgggcc acgcgtaaaa ggtagttatt gatatcaaag gcccatgggg atcggctgtg
     7021 ggcctgtgtt aattagtggt tattcgctgc cagatcggct tcgcttagct gagtggccgc
     7081 gagcacctgg tcacgatcaa tgccctgttc cagcatgcgc aaggcaattt taatggcttg
     7141 ttcatgcatg ccttctaatt taccctcctg ccagccaatt tggtgtcctt cctgccgtag
     7201 ccgttcagca atagtcatta atctctcctt ttaataacag ttcggaatat cagcatcagt
     7261 gagttgggtt gtcgccagca caatctcacg ttcaaagccc tgttccagca tgcgcaaggc
     7321 aattttaatg gcttgttcat gcataccttc tactttgcct ttctgcaaac cctccaactt
     7381 cccctcctgc caaccttcaa tcttaccctt ctgccagcca atttgatgcc cttcctgccg
     7441 cagccgttca gcaatagtca ttaatctctc cttttgtagt ggtgaacgtt cggcaatctc
     7501 ctcaataaaa cgggtgaaac gggaggtatc gccgcattgc agcagataat taaacagcgt
     7561 ttgtagctgg ctgtcattag tgaagcctct aaccaaaagc gtggtgatcc tgtcgaccat
     7621 accgattaaa tcgcggtcgc gaatatgctt ttgaatcagt tccagcagag ctatacgccg
     7681 atgttgcatg atctcatcgt caggtacgat ggtgatatcc accaacggaa aagcttcggt
     7741 gtacaactgc cgggcaagtt gcggatcgtc aaactcatcc agccagttga gtgagtacgg
     7801 gtagggcgag gtttcgccat gataaaacag caatggtacc accagcggga ctctgtcata
     7861 gcctttgtcc aggtgacgct gcatggcggc agtggcatag cgcattagcc gaaaagccat
     7921 attcttttct gcagagcttt gatgttcaat cacgcagtag atataaccgt caccctcgct
     7981 ggtttccaca gaccacaaaa catccgagta gtaagcgcgt aaactttttt caataaagct
     8041 ggtgggttcc aggcgtaagg tttgcaggtt gcaaagcttg cgcagcggct ctggtagatg
     8101 tatttccaga aaatctcgcg ccgtatcggg cgagaacata aaggttttaa ataccgcatc
     8161 atgcggggag gaggttgttg attcggtcat ggttgtccgt cacctgatat ttatgatgac
     8221 gcgacattac ggttatcccg ggcctttgac atctgccagt ttcagccttt gcgagacgtt
     8281 ttcagaggaa ttttcactgc gttgcaacgt atacattggg cacggaatcg tgtgcgcaaa
     8341 aatgcccggt aaccacatgc ttaccgggca tcagaaggga gtttaagcca gtgcctgggc
     8401 cagcagcgta atcggatgtt cgcagcgaag acttgtggac atctcaatct gccatttaca
     8461 ggtttcgcag tcggtgacca ccagatctgc gccgctctct tctatctggc ggaacagtgg
     8521 tgcgccgatg gcttgtgaag tggggtagtt ctcttttttg aaaccgtaag ttcccgcaat
     8581 accgcagcac tgggaatcca gcaccgttaa ctcaagcccc gggattttac gcaacagctc
     8641 cagggtgtag agcgtccagc ccattttttc catatggcac ggagtgtgat aaaccacttt
     8701 cagcggcagc gttttcagcg gtaacgtttt gccttcgtcc agcttgcgcc acagccagcg
     8761 ggtcgccagt tcgatatgat cgcgcaagcc tttgttgtct acattcagca cttctgggta
     8821 ttcgtcgcgc agggcaaatg tacaagttga ggaggtggca atcaccggaa tgccttttac
     8881 tcccacagct tcgcggatcg actctacatt cgtaattgcc tgtttacgtg ctttatcggt
     8941 aaagccgttg gcgatcagcg gtacgccgca gcatttttct ttgctgagca gttgtacacc
     9001 ggtacccatt gcgttgagca ctttaattaa atctttacct aactgcggat ggttgtagtt
     9061 aacgaagcag ccgtgaaaga aagcgacctg gtctttatac tgcgcttgct gagccgccac
     9121 gctgcgatac cagcgacgga acgtaccgaa ggagtatttc ggtagcgtgc ggcgatgatc
     9181 gatttttaac gccgcatcaa gcagctgccg cactggtttc agcgaggtag cggtattgac
     9241 gattggtgcg aatggcgtgg aaacgctacc catcaggtcg gtatgactca acacaaaatt
     9301 gcgcagcgac gggcgtgtgg tgtcatattt cgcccgcgcg cgctggatga tatcgccaat
     9361 cttcacatcg gacgggcagg cgacttcgca acgtttgcag ttgatgcaat atttcagtgc
     9421 ctcgtcatac agtgcgccat ctttcaaacg cagacgctcg ccatccggcc cggcttgttt
     9481 tggccccgga tatcccggat tcacccggct caccgggcag gcggtggtgc agacggtgca
     9541 cttaatgcag ttttcgaagc tggtgtcgtt cattgttgac ctcctgcgcg ttgggcaatc
     9601 tgttgagcgg catgtaaagc actgacggca caaacaccgc cgccgcatcc ctgggcgatg
     9661 ggatcaaatc cgcccagcac cgaaccgatg gcaaacaggt tctcaatggt ttgccctgcc
     9721 tgcgacgggc gtagtgcctc atcagtggtt acaccgaact gctgccacgg ttgcggcgca
     9781 aaaaaatcgc ccttatacca ttcaccccgc gtggcggttt gtagcacatc aaggccgaga
     9841 atcggctctc gaatgccgtt acgttccgcc accagtccgc cgctaaagaa actgccgctg
     9901 gcgagcacgg caaagcgtgg acgcagcggg atatcggcgt gattgcgcgt ccagatttca
     9961 tttactacgc catttttaca ggtcactttt ttcacttcat cgcctggcat ccacacgcca
    10021 ccctggcgca caaactggcg ctgtaactgg ttttgcagac gaatgcccag cacggaaggc
    10081 ggcagcgttg gcaaaagcat cagtgaacaa ggtagttttt cattcaacca atgccacagt
    10141 ttgtcatcgg ccaggccgaa gcaggcgggc atcaggatca tttcgcaggt attggcgaca
    10201 ggaataagcg catcaagtaa cagcggccag ttctcttcgt tatcaaggaa acgggcgata
    10261 ttcaccgcgc gaaactcggt ggcgttgttg cgcagcacat ccagttccgg cagttcaatt
    10321 tctgcggttt caacggcaag gtcgagttcg cgcaacgaag ccgccgcaag atgcgcctga
    10381 aaatccatca gaccgctaat tcctactaca catattttct tcgcgggcag cggccagacg
    10441 gggacttctg gcgcacttag ccaggtagaa cgcagggtac cgagcggggt aacacgctgg
    10501 tgggccagtt ctacgctgcc ctgtaattgc gcaccgcttt cagcgattaa tgcctgcgcc
    10561 tggcaagcga gatcgagtac gcgttgtggc ccgagaagag tgtaaggatg ggctggagcc
    10621 tgctgacgca aagattccag tccactgtga atgtctgtca ccggttgacc atccggcaga
    10681 tggctcagca aatccagcga accggatgag aaatgcagcg cgctttgacc acgagtgaca
    10741 atggcacagc gcaggccgtg tttttgcagt tgcaggccac agagtaatcc ggcgaggccg
    10801 ccgcccataa tgacagtatc aaagcgcatc tttctgctcc ttctccagac cacacagtcc
    10861 ctgataaacc cagcgggtaa attcgctttc gcgcagtgcg tctccccatg ctatgggttg
    10921 cacgcctttc cagcgttcgt taaggaaggt ggaaagttgc tcgatagatt gcgcggacgt
    10981 cgtgacgtta aaacgttgca gcagtccggc agcccggcag gcgcagagtt cgccctggca
    11041 ggtacccatc cctacacggg tacgacggcg taaatccagc aggctgttaa cgtttaaatt
    11101 ttctactgcg tactgcactt cacccgcagt taccgcttcg cactcacata ccaggctgcg
    11161 gtgcaggcgg ccttcgctca gccaggctgg tgtgcgatcg ccgtgccggt aaactgcgga
    11221 accgcgcagc ggagcgggca gggagatcac tttgcgcagc gtgacttctg ccggttcctg
    11281 tgagccaggc aacggcgtgt cggcagtggt acaggggcgc gtgttgccca gcttgcgaca
    11341 caccgcgtca gtagcccatt ccgccatcag gcggtaggtc atcagtttgc caccggtgat
    11401 ggtgataaac ccgtccagac catcgcgttc agcatggtcg agcagcacga tgccacggct
    11461 gacgttacgt ccgctcggat cgtcatcgct ggcaaccagc gggcgtacgc cagagtaggc
    11521 tcgcaaaatg cgcgttttcg ccatcacggg ggccagtttt tccccttcac gcagcagaat
    11581 atcaacttct tctgccgtca ctcgattatc gtcaatctcg ctgtaatcaa tacgtaaaga
    11641 ggtggtgcca attagcgaaa tggtatcgcc aggcaccaga atatcggcgt cggaaggttt
    11701 gcggcagcgg ttgatcacat gctgattaat gcggtgatcc atgatcagca gcgatccttt
    11761 cgccgggaac atgcgaatgc gcagatcggc atattcggca atgtgttgcc cccagatccc
    11821 ggcggcatta actacgacag gcgcatgaag tgcctgaatt tcgccggtga gatggttacg
    11881 tacacgaaca ccgcacaccg tcgcgccttc acgaatcagc cccgtgactt catgagcagt
    11941 aaggataacg gcaccgtgtt ctttggcatc aagcatattt gctgcggtca gacgaaatgg
    12001 atcaacggtg ccatccggaa ctttcaccgc gccaatcagt gccgggttaa cggcaggttc
    12061 gataatgcgc gcttgctgcg ggtctatagc ttctgcgctg atccctgctt cttcgcaggc
    12121 gcggataaaa gtggcctgga aggagaggtc atcttccggc agggtgataa acaggccgtt
    12181 ggtcggttca acgcagtgac gcgcaatgcg tttcaggatc tggttttcac taatacattc
    12241 gcgggccgat tccgcatcgg ttaccgcata gcgcgcaccg ctgtgtagca ggccgtggtt
    12301 acgcccggtg gcaccggttg cgatgtcgtg gcgctctacc aaaatcacgc gcagcccgcg
    12361 cagggcacag tcgcgggcaa tcccggctcc cgttgcgccg ccgccaatga taatcacgtc
    12421 acttgattgc gagtcgcgag ttttcattgt tatccctctg aagttcgttt ttaccattta
    12481 gccatagtaa aaacatgaat tgtttgattt cgcgcatatt cgctcataat tcgaaagtga
    12541 aacgtgattt catgcgtcat tctgaacatt taaaccatcg tatttaataa tgtgtgcggt
    12601 aattcacatt taatttatga atgttttctt aacatcgcgg caactcaaga aacggcaggt
    12661 tctcccactg aatcagtctg ttaatcataa ataagaccac gggccacgga ggctttcaat
    12721 gttgagtatt tttaaaccag cgccacacaa agcgcgctta cctgctgcgg agatcgatcc
    12781 gacttatcgt cgattgcgct ggcaaatttt cctggggata ttctttggct atgctgctta
    12841 ctatttggtt cgtaagaact ttgcgctcgc tatgccttat ctggttgagc agggattctc
    12901 acgcggtgat ttaggttttg ccctttcggg gatctcgatt gcttatggat tttcgaaatt
    12961 catcatgggt tcggtatcgg atcgctcgaa tccgcgcgtt ttcctgcccg caggtttgat
    13021 tctggcggcg gcagtgatgt tgtttatggg ctttgtgccg tgggcgacgt cgagcattgc
    13081 ggtgatgttt gtactgttgt tcctctgcgg ttggttccag gggatgggat ggccgccgtg
    13141 cggtcgtact atggtgcact ggtggtcgca gaaagaacgt ggcggcattg tgtcagtgtg
    13201 gaactgtgcg cacaacgtcg gtggtggtat tccgccgctg ctgttcctgc tggggatggc
    13261 ctggttcaat gactggcacg cggcgctcta tatgcccgct ttctgcgcca ttctggtggc
    13321 actgttcgcc tttgcgatga tgcgcgatac cccgcaatcc tgtggcctgc cgccgatcga
    13381 agagtacaaa aatgattatc cggacgacta taacgaaaaa gcggaacagg agctgacggc
    13441 gaagcaaatc ttcatgcagt acgtgctgcc gaacaaactg ctgtggtaca tcgctatcgc
    13501 caacgtgttc gtttatctgc tgcgttacgg catcctcgac tggtcaccga cttatctgaa
    13561 agaggtgaaa cacttcgcgc tggataaatc ctcctgggcc tacttcttct atgagtatgc
    13621 gggcattccg ggcaccctgc tgtgcggctg gatgtcggat aaagtcttcc gtggcaaccg
    13681 tggggccacc ggtgtcttct ttatgacact ggtgaccatc gcgactatcg tttactggat
    13741 gaacccggca ggtaacccaa ccgtcgatat gatttgtatg attgttatcg gcttcctgat
    13801 ctatggccct gtgatgctga tcggtctaca tgcgctggaa ctggcaccga aaaaagcggc
    13861 aggtacggca gcgggtttta ccgggctgtt tggttacctc ggcggttcgg tggcggcgag
    13921 cgcgattgtt ggctacaccg tggacttctt cggctgggat ggcggcttta tggtaatgat
    13981 tggcggcagc attctggcgg ttatcttatt gattgttgtg atgattggcg aaaaacgtcg
    14041 ccatcaggaa ttactgcaaa aacgcaacgg aggctaatgg catgaaattg acgctgaaaa
    14101 accttagcat ggcgatcatg atgagtggaa tgatcatggg aagcagtgca atggcggcgg
    14161 acagcaacga aaaaatagtc atcgcccatc gcggtgccag tggatatttg ccggagcata
    14221 cgctgccagc aaaagcgatg gcgtatgcgc agggagcgga ttatctggaa caggatttgg
    14281 tgatgaccaa agacgaccat ctggttgttc tgcatgacca ttatctcgat cgtgttactg
    14341 atgttgccga tcgtttcccg gatcgggcgc gcaaagacgg tcgttactac gcgatagatt
    14401 tcacgctcga tgaaattaag tcactgaaat ttaccgaagg tttcgatatt gaaaacggta
    14461 agaaagtaca gacttatccg gggcgtttcc caatgggtaa gtccgacttc cgggtgcaca
    14521 cctttgaaga agagattgaa tttgttcagg ggttaaatca ctctaccggg aaaaatattg
    14581 gtatctaccc ggagattaaa gcgccgtggt tccatcatca ggaagggaag gatattgcgg
    14641 caaaaacgct ggaagtgctg aagaaatatg gttacaccgg taaggacgac aaagtttatt
    14701 tgcaatgttt tgatgccgat gaactgaagc gtattaagaa tgagctggaa cctaaaatgg
    14761 gcatggacct caatctggta cagctgattg cctataccga ctggaacgaa actcagcaga
    14821 aacagccgga cggaagctgg gttaattaca actacgactg gatgtttaag ccgggggcca
    14881 tgaaacaggt ggcggaatat gctgatggta ttggtccgga ttaccatatg ttgattgagg
    14941 agacatcgca gccaggtaat atcaaactca ctggcatggt gcaagatgct cagcagaata
    15001 aactggtagt gcatccttac accgtgcggt cagataagct gcctgaatac actactgatg
    15061 tgaatcagtt atatgatgct ctgtataaca aagcgggtgt aaatgggctg tttactgatt
    15121 tccctgataa ggcagtaaaa ttccttaata aagagtaatg ctactgcagg atggcgttca
    15181 tgcgccatcc tgtctcatat caaattttcc actctccact aattgttctg tgcacatttg
    15241 tagtaaaaac agtggactgc cttctaccgg cttttcttta gccagcaacg aaatctgtcg
    15301 atagaaggtt ggttcgagag gaacggaaca caattcatct gcaatggttt tcagcgttaa
    15361 ctctggtaat aaggcgatgc ctaatccctg tcgaataaaa ctgatcgctg tatccgggtg
    15421 attaaattca tattttatct gtggggtaat attcttttct ttaaatagtg ccataatgct
    15481 taattcatag cgtcctttac taataattaa tggttcaccc attaactctt ccactgtaat
    15541 agtgctgttt tttgccagag catggttggg cggtaacact acggtgaatt tatcctgata
    15601 aatgggcacg gagtacattc cgctcaccgg aaaatgaaca aaaccggcat caatggattc
    15661 attctgaagg gaatcaataa tcgctgtgct attttcctca aaaggaataa tttttatatt
    15721 cggatgatga atttcaaagt agcgaatcac agccggtaag atacaggcac agacgctggg
    15781 gaaacaacca agtctcagcg ttctttgagg gttctgcttt tcctgctcgg ctatctcttt
    15841 tactgcgttc acttcccgat taatggcgcg cagatgatgc ataatgcggt gacctgcaga
    15901 ggtcagttgt atagctttac ggcgatcgcg taacagtata ttcacgccaa gttcttgttc
    15961 gagggcagca attgcctgac ttaccgccga ctgcgtcatg aacaatcgct tgctggcttc
    16021 agtaaagcca ccgtaatcca gcacggcgag taaagaattt atttgggtaa gagtcattag
    16081 agtttgctaa tcaataatat taaaacgatt aatttctcta atagcatgcc gccatttata
    16141 cttctgttct gcaaagtaca tcacaatttg acgtaaccgc agtaacggga gtgttgtctg
    16201 cgtgcgtcat cctgacaggc ggtagggtat gaaagagaag ttatggacga aggatttttg
    16261 ggcaataacc atcatcagtt ttattatttt cttcgtcttt tatgttttac taacattgtt
    16321 gccaatttat atctcagacc gcttgcatgc ctctcctgat aaagcaggtt tgttggtgac
    16381 tttattttta attgccgcca ttgttattcg cccctttgcc gggcaatggg tgggtaaata
    16441 ttcgaataaa actattctgg ggctctcttc tctggccttc ttggtggtca ctgcgctgta
    16501 tcctttttgc cactcaattg aatcactgct ttttattcgg gtgcttcatg gtattacctt
    16561 cggggttatc actacggtaa aaggaacgat ttccgcgcgg ctgatcccgg cctcccgacg
    16621 tggggagggc atcagttttt tctctctggc aatggggctg gcaatggtgg tcgggccgtg
    16681 gattggcctg aatatggcgc gctgggaggc ctttaatatg gctttctggt tatgcaccgg
    16741 cgtggcagcg gtggggatta ttctgtcgct gattatgaca gtgccgccgg ttatcagcca
    16801 tgctgatggt tcaacgccga aaatgggctt cgccgccatg ttcgatcgcg cggcgttgcc
    16861 atttgcgatg gtcacctttt ttatgacctt ttcgtatgcc ggggtttc
//
LOCUS       DAMZFT010000108        16781 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.108, whole genome shotgun sequence.
ACCESSION   DAMZFT010000108 DAMZFT010000000
VERSION     DAMZFT010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16781)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16781)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16781
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.108"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..348
                     /gene="fliH"
                     /locus_tag="PN977_003648"
     CDS             <1..348
                     /gene="fliH"
                     /locus_tag="PN977_003648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707825.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliH"
                     /protein_id="HDI6043647.1"
                     /translation="SVIASRLMQMALEAARQVIGQTPTVDNSALIKQIQQLLQQEPLF
                     SGKPQLRVHPDDLQRVDDMLGATLSLHGWRLRGDPTLHPGGCKVSADEGDLDASVATR
                     WQELCRLAAPGVV"
     gene            348..1721
                     /gene="fliI"
                     /locus_tag="PN977_003649"
     CDS             348..1721
                     /gene="fliI"
                     /locus_tag="PN977_003649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellum-specific ATP synthase FliI"
                     /protein_id="HDI6043648.1"
                     /translation="MTTRLTRWLTTLDNFEAKMAQLPAVRRYGRLTRATGLVLEATGL
                     QLPLGATCVIERQNGSETHEVESEVVGFNGQRLFLMPLEEVEGVLPGARVYAKNISAE
                     GLQSGKQLPLGPALLGRVLDGSGKPLDGLPSPDTTETGALITPPFNPLQRTPIEHVLD
                     TGVRPINALLTVGRGQRMGLFAGSGVGKSVLLGMMARYTRADVIVVGLIGERGREVKD
                     FIENILGAEGRARSVVIAAPADVSPLLRMQGAAYATRIAEDFRDRGQHVLLIMDSLTR
                     YAMAQREIALAIGEPPATKGYPPSVFAKLPALVERAGNGISGGGSITAFYTVLTEGDD
                     QQDPIADSARAILDGHIVLSRRLAEAGHYPAIDIEASISRAMTALISEQHYARVRTFK
                     QLLSSFQRNRDLVSFGAYAKGSDPMLDKAIALWPQLEGYLQQGIFERADWEASLQGLE
                     RIFPTVS"
     gene            1740..2183
                     /gene="fliJ"
                     /locus_tag="PN977_003650"
     CDS             1740..2183
                     /gene="fliJ"
                     /locus_tag="PN977_003650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis chaperone FliJ"
                     /protein_id="HDI6043649.1"
                     /translation="MAEHGALATLKDLAEKEVEDAARLLGEMRRGCQQAEEQLKMLID
                     YQNEYRNNLNSDMSAGMTSNRWINYQQFIQTLEKAITQHRQQLNQWTQKVDIALNSWR
                     EKKQRLQAWQTLQERQSTAALLAENRLDQKKMDEFAQRAAMRKPE"
     gene            2180..3307
                     /gene="fliK"
                     /locus_tag="PN977_003651"
     CDS             2180..3307
                     /gene="fliK"
                     /locus_tag="PN977_003651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook length control protein FliK"
                     /protein_id="HDI6043650.1"
                     /translation="MIRLAPLITADVDTTTLPGGKASDAAQDFLALLSEALAGETTTD
                     KAAPQLLVATDKPTTKGEPLVSEILADAQQADLLIPVDETPPVINDEQSTSTPLTTAQ
                     TMTLAAVAGNNTAKDEKADDLNEDVTASLSALFAMLPGFDNTPKVTDAPSTVLPTEKP
                     TLFTKLTSEQLTTAQPDDAPGTPAQPLTPLVAEAQSKAEVISTPSPVTAAASPLITPH
                     QTQPLPTVAAPVLSAPLGSHEWQQSLSQHISLFTRQGQQSAELRLHPQDLGEVQISLK
                     VDDNQAQIQMVSPHQHVRAALEAALPVLRTQLAESGIQLGQSNISGESFSGQQQAASQ
                     QQQSQRTVNHEPLAGEDDDTLPVPVSLQGRVTGNSGVDIFA"
     gene            3412..3876
                     /gene="fliL"
                     /locus_tag="PN977_003652"
     CDS             3412..3876
                     /gene="fliL"
                     /locus_tag="PN977_003652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated protein FliL"
                     /protein_id="HDI6043651.1"
                     /translation="MTDYAISKKSKRSLWIPILVFITLAACASAGYSYWHSHQVAADD
                     KAQQRVVPSPVFYALDTFTVNLGDADRVLYIGITLRLKDEATRSRLSEYLPEVRSRLL
                     LLFSRQDAAVLATEEGKKNLIAEIKTTLSTPLVAGQPKQDVTDVLYTAFILR"
     gene            3881..4885
                     /gene="fliM"
                     /locus_tag="PN977_003653"
     CDS             3881..4885
                     /gene="fliM"
                     /locus_tag="PN977_003653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HDI6043652.1"
                     /translation="MGDSILSQAEIDALLNGDSEVKDEPTASVSGESDIRPYDPNTQR
                     RVVRERLQALEIINERFARHFRMGLFNLLRRSPDITVGAIRIQPYHEFARNLPVPTNL
                     NLIHLKPLRGTGLVVFSPSLVFIAVDNLFGGDGRFPTKVEGREFTHTEQRVINRMLKL
                     ALEGYSDAWKAINPLEVEYVRSEMQVKFTNITTSPNDIVVNTPFHVEIGNLTGEFNIC
                     LPFSMIEPLRELLVNPPLENSRNEDQNWRDNLVRQVQHSQLELVANFADISLRLSQIL
                     KLKPGDVLPIEKPDRIIAHVDGVPVLTSQYGTLNGQYALRIEHLINPILNSLNEEQPK
                     "
     gene            4882..5295
                     /gene="fliN"
                     /locus_tag="PN977_003654"
     CDS             4882..5295
                     /gene="fliN"
                     /locus_tag="PN977_003654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
                     /protein_id="HDI6043653.1"
                     /translation="MSDMNNPADDNNGAMDDLWAEALSEQKSTNSKSAADAVFQQFGG
                     GDVSGTLQDIDLIMDIPVKLTVELGRTRMTIKELLRLTQGSVVALDGLAGEPLDILIN
                     GYLIAQGEVVVVADKYGVRITDIITPSERMRRLSR"
     gene            5298..5663
                     /gene="fliO"
                     /locus_tag="PN977_003655"
     CDS             5298..5663
                     /gene="fliO"
                     /locus_tag="PN977_003655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FliO"
                     /protein_id="HDI6043654.1"
                     /translation="MNNHATVQSSAPVSAAPLLQVSGALIAIIALILAAAWLVKRLGF
                     APKRTGVNGLKISASASLGARERVVVVDVDDARLVLGVTAGQINLLHKLPPSAPTEEI
                     PQTDFQSVMKNLLKRSGRS"
     gene            5663..6400
                     /gene="fliP"
                     /locus_tag="PN977_003656"
     CDS             5663..6400
                     /gene="fliP"
                     /locus_tag="PN977_003656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310714.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HDI6043655.1"
                     /translation="MRRLLSVAPVLLWLITPLAFAQLPGITSQPLPGGGQSWSLPVQT
                     LVFITSLTFIPAILLMMTSFTRIIIVFGLLRNALGTPSAPPNQVLLGLALFLTFFIMS
                     PVIDKIYVDAYQPFSEEKISMQEALEKGAQPLREFMLRQTREADLGLFARLANTGPLQ
                     GPEAVPMRILLPAYVTSELKTAFQIGFTIFIPFLIIDLVIASVLMALGMMMVPPATIA
                     LPFKLMLFVLVDGWQLLVGSLAQSFYS"
     gene            6410..6679
                     /gene="fliQ"
                     /locus_tag="PN977_003657"
     CDS             6410..6679
                     /gene="fliQ"
                     /locus_tag="PN977_003657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013365797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HDI6043656.1"
                     /translation="MTPESVMMMGTEAMKVALALAAPLLLVALVTGLIISILQAATQI
                     NEMTLSFIPKIIAVFIAIIIAGPWMLNLLLDYVRTLFTNLPYIIG"
     gene            6688..7473
                     /gene="fliR"
                     /locus_tag="PN977_003658"
     CDS             6688..7473
                     /gene="fliR"
                     /locus_tag="PN977_003658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FliR"
                     /protein_id="HDI6043657.1"
                     /translation="MMQVTSDQWLSWLSLYFWPLLRVLALISTAPILSERSVPKRVKL
                     GLAMMITFAIAPSLPANDVPVFSFFALWLAVQQILIGIALGFTMQFAFAAVRTAGEII
                     GLQMGLSFATFVDPASHLNMPVLARIMDMLALLLFLTFNGHLWLISLLVDTFHTLPIG
                     GEPLNSNAFLALTKAGSLIFLNGLMLALPLITLLLTLNLALGLLNRMAPQLSIFVIGF
                     PLTLTVGISLMAALMPLIAPFCEHLFSEMFNLLADIISELPLI"
     gene            7763..8386
                     /gene="rcsA"
                     /locus_tag="PN977_003659"
     CDS             7763..8386
                     /gene="rcsA"
                     /locus_tag="PN977_003659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator RcsA"
                     /protein_id="HDI6043658.1"
                     /translation="MSTIIMDLCSYTRLGLTGYLLSRGVKKREINDIETVDDLAIACD
                     SQRPSVVFINEDCFIHDASNSQHIKHIINQHPNTLFIVFMAIANVHFDEYLLVRKNLL
                     ISSKSIKPESLDDILGDILKKETTITSFLNMPTLSLSRTESSMLRMWMAGQGTIQISD
                     QMNIKAKTVSSHKGNIKRKIKTHNKQVIYHVVRLTDNVTNGIFVNMR"
     gene            complement(8430..8618)
                     /gene="dsrB"
                     /locus_tag="PN977_003660"
     CDS             complement(8430..8618)
                     /gene="dsrB"
                     /locus_tag="PN977_003660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein DsrB"
                     /protein_id="HDI6043659.1"
                     /translation="MKVNDRVTVKTDGGPRRPGVVLAVEEFSEGTMYLVSLEDYPLGI
                     WFFNEAGHQDGIFVEKAE"
     gene            8781..9008
                     /gene="yodD"
                     /locus_tag="PN977_003661"
     CDS             8781..9008
                     /gene="yodD"
                     /locus_tag="PN977_003661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416463.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid resistance protein YodD"
                     /protein_id="HDI6043660.1"
                     /translation="MKTAKEYSDTAKREVSVDVDALLAAINEISESEVHRSQNDSEHV
                     SVDGREYHTWRELADAFELDIHDFSVSEVNR"
     gene            9305..10120
                     /locus_tag="PN977_003662"
     CDS             9305..10120
                     /locus_tag="PN977_003662"
                     /EC_number="3.1.3.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-3-phosphoglycerate phosphatase-related
                     protein"
                     /protein_id="HDI6043661.1"
                     /translation="MFSIQQPLLVFSDLDGTLLDSHSYDWQPAAPWLSRLREGNVPVI
                     LCSSKTSAEMLYLQKTLGLQGLPLIAENGAVIQLAEQWQDIDGFPRIISGISHGEISQ
                     VLNTLREKEHFKFTTFDDVDDATIAEWTGLSRSQAALTQLHEASVTLIWRDSDERMAQ
                     FTARLNELGLQFMQGARFWHVLDASAGKDQAANWIIATYQQLSGKRPTTLGLGDGPND
                     APLLEVMDYAVIVKGLNREGVHLHDEDPTRVWRTQREGPEGWREGLDHFFSAR"
     gene            complement(10117..11811)
                     /gene="yedQ"
                     /locus_tag="PN977_003663"
     CDS             complement(10117..11811)
                     /gene="yedQ"
                     /locus_tag="PN977_003663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis regulator YedQ"
                     /protein_id="HDI6043662.1"
                     /translation="MQHETKMENQSWLKKLARRLGPGHVVNLCFIVVLLFSTLLTWRE
                     VVVLEDAYISSQRNHLENVANALDKHLQYNVDKLIFLRNGMREALVALLDFTSLRNAV
                     TEFEQHRDEHAWQIELNRRRTLPVNGVSDALVSEGNLLSRENESLDNEITAALEVGYL
                     LRLAHNSSSMVEQAMYVSRAGFYVSTQPTLFTRNVPTRYYGYVTQPWFIGHSQRENRH
                     RAVRWFTSQPEHASNTEPQVTVSVPVDSNNYWYGVLGMSIPVRTMQQFLRNAIDKNLD
                     GEYQLYDSKLRFLTSSNPDHPTGNIFDPRELALLAQAMEHDTRGGIRMDSRYVSWERL
                     DHFDGVLVRVHTLSEGVRGDFGSISIALTLLWALFTTMLLISWYVIRRMVSNMYVLQS
                     SLQWQAWHDTLTRLYNRGALFEKARPLAKLCQTHQHPFSVIQVDLDHFKAINDRFGHQ
                     AGDRVLSHAAGLISSSLRAQDVAGRVGGEEFCVILPGASLTEAAEVAERIRLKLNEKE
                     MLIAKSTTIRISASLGVSSSEETGDYDFEQLQSLADRRLYLAKQAGRNRVCASDNA"
     gene            complement(11982..12164)
                     /locus_tag="PN977_003664"
     CDS             complement(11982..12164)
                     /locus_tag="PN977_003664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2158 domain-containing protein"
                     /protein_id="HDI6043663.1"
                     /translation="MSFMVSEEVTVKEGGPRMIVTGYSSGMVECRWYDGYGVKREAFH
                     ETELVPGEGSRSAEEV"
     gene            complement(12243..13160)
                     /locus_tag="PN977_003665"
     CDS             complement(12243..13160)
                     /locus_tag="PN977_003665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 domain-containing protein"
                     /protein_id="HDI6043664.1"
                     /translation="MLLAGSSLLTLLDDIATLLDDISVMGKLAAKKTAGVLGDDLSLN
                     AQQVSGVRANRELPVVWGVAKGSLINKVILVPLALIISAFIPWAITPLLMIGGAFLCF
                     EGVEKVLHMLEARKHKEDPAQSQQRLEKLAAQDPLKFEKDKIKGAIRTDFILSAEIVA
                     ITLGIVAEAPLLNQVLVLSGIALVVTVGVYGLVGVIVKIDDLGYWLAEKSSALMQALG
                     KGLLIIAPWLMKALSIVGTLAMFLVGGGIVVHGIAPLHHAIEHFAGQQSAVVAMILPT
                     VLNLILGFIIGGIVVLGVKAVAKIRGQAH"
     gene            13333..14253
                     /gene="yedA"
                     /locus_tag="PN977_003666"
     CDS             13333..14253
                     /gene="yedA"
                     /locus_tag="PN977_003666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="drug/metabolite exporter YedA"
                     /protein_id="HDI6043665.1"
                     /translation="MRFRQLLPLFGALFALYIIWGSTYFVIRIGVESWPPLMMAGVRF
                     LAAGILLMAFLLLRGHKLPPLRPLLNAALIGLLLLAVGNGMVTVAEHQNVPSGIAAVV
                     VATVPLFTLCFSRLFGIKTRKLEWVGIAIGLAGIIMLNSGGNLSGNPWGAILILIGSI
                     SWAFGSVYGSRITLPVGMMAGAIEMLAAGVVLMIASMIAGEKLTALPSLSGFLAVGYL
                     ALFGSIIAINAYMYLIRNVSPALATSYAYVNPVVAVLLGTGLGGETLSKIEWLALGVI
                     VFAVVLVTLGKYLFPAKPVVAPVIQDASSE"
     gene            complement(14242..14712)
                     /gene="vsr"
                     /locus_tag="PN977_003667"
     CDS             complement(14242..14712)
                     /gene="vsr"
                     /locus_tag="PN977_003667"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VSPR family DNA mismatch endonuclease"
                     /protein_id="HDI6043666.1"
                     /translation="MADVHDKATRSKNMRAIATRDTAIEKRLASLLTGQGLAFRVQDA
                     SLPGRPDFVVDEYRCVIFTHGCFWHHHHCYLFKVPATRTEFWLEKIGKNVERDRRDIS
                     RLQELGWRVLIVWECALRGREKLTDEALSERLEEWICGEGASAQIDTQGIHLLA"
     gene            complement(14693..16111)
                     /gene="dcm"
                     /locus_tag="PN977_003668"
     CDS             complement(14693..16111)
                     /gene="dcm"
                     /locus_tag="PN977_003668"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-cytosine methyltransferase"
                     /protein_id="HDI6043667.1"
                     /translation="MQENISVTDSYSTGNAAQAMLEKLLQIYDVKTLVAQLNGVGENH
                     WSAAILKRALANDSAWHRLSEKEFAHLQTLLPKPPAHHPHYAFRFIDLFAGIGGIRRG
                     FESIGGQCVFTSEWNKHAVRTYKANHYCDPATHHFNEDIRDITLSHKEGVSDEAAAEH
                     IRQHIPEHDVLLAGFPCQPFSLAGVSKKNSLGRAHGFACDTQGTLFFDVVRIIDARRP
                     AMFVLENVKNLKSHDQGKTFRIIMQTLDELGYDVADAEDNGPDDPKIIDGKHFLPQHR
                     ERIVLVGFRRDLNLKADFTLRDISECFPAQRVTLAQLLDPMVEAKYILTPVLWKYLYR
                     YAKKHQARGNGFGYGMVYPNNPQSVTRTLSARYYKDGAEILIDRGWDMATGEKDFDDP
                     QNQQHRPRRLTPRECARLMGFEAPGEAKFRIPVSDTQAYRQFGNSVVVPVFAAVAKLL
                     EPKIKQAVALRQQEAQHGRRSR"
     gene            complement(16178..>16781)
                     /locus_tag="PN977_003669"
     CDS             complement(16178..>16781)
                     /locus_tag="PN977_003669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HDI6043668.1"
                     /translation="RVWATAQKLAADDDVDMLVILTACYFHDIVSLAKNHPQRQRSSI
                     LAAEETRRLLREEFEQFPAEKIEAVCHAIAAHSFSAQIAPLTTEAKIVQDADRLEALG
                     AIGLARVFAVSGALGVALFDGEDPFAQHRPLDDKRYALDHFQTKLLKLPQTMQTARGK
                     QLAQHNAHFLVEFMAKLSAELAGENEGVDHKVIDAFSPAG"
BASE COUNT         3996 a         4482 c         4267 g         4036 t
ORIGIN      
        1 agtgtgattg cttcgcgcct gatgcagatg gcgctggagg cggcacgtca ggtgatcggt
       61 cagacgccaa cagtggataa ctcggcactg atcaaacaga tccaacagtt gttgcagcaa
      121 gaaccgttat tcagcggtaa accacagctg cgcgtgcacc cggatgatct gcaacgtgtg
      181 gatgatatgc tcggcgcaac cttaagtttg catggctggc gcttgcgggg cgatcccaca
      241 ctccatcctg gcggctgtaa agtctccgcc gatgaaggcg atctcgacgc cagtgtcgcc
      301 actcgctggc aagaactctg ccgtctggca gcaccaggag tggtgtaatg accacgcgcc
      361 tgactcgctg gctaaccacg ctggataact ttgaagccaa aatggcgcag ttgcctgcgg
      421 tacgtcgcta tgggcgatta actcgcgcta ccgggctggt gctggaagcc accggattac
      481 aattgccgct cggcgcaacc tgtgtcattg agcgccagaa cggcagcgaa acgcacgaag
      541 tagaaagcga agtcgttggc tttaacggtc aacggctgtt tttaatgccg ctggaggaag
      601 tcgaaggtgt cctgcccggc gcgcgtgttt atgccaaaaa catttcggca gaagggctgc
      661 aaagcggcaa gcagttaccg ctcggtccgg cgttattagg tcgtgttctg gacggcagcg
      721 gtaaaccgct cgatggcctc ccctcccccg atacgacgga aaccggtgcg ctgattaccc
      781 cgccatttaa cccgttgcaa cgtacaccga ttgaacatgt gctggacacc ggcgtgcgcc
      841 caatcaatgc cctgcttacc gttgggcgcg ggcagcgtat ggggctgttt gccggatctg
      901 gcgttggtaa aagtgtgctg ctggggatga tggcccgtta cacccgcgcc gatgtcattg
      961 tcgtgggttt gattggtgaa cgtgggcgtg aagtaaaaga ttttattgag aatatcctcg
     1021 gtgctgaagg gcgtgcacgc tcagtagtga ttgccgctcc ggcggatgtt tctcctctcc
     1081 tgcgaatgca gggtgccgcc tatgccacgc gcattgccga agattttcgc gatcgtggtc
     1141 agcatgtgtt gctgattatg gactccctca cccgctacgc gatggcccag cgtgaaattg
     1201 cgctggcgat tggcgaacca cccgccacta aaggttatcc cccgtcggtg tttgccaaat
     1261 taccggcact ggtcgagcgt gccggaaatg gcattagcgg cggcggctcg attaccgcgt
     1321 tttataccgt gctcaccgag ggcgatgacc agcaggaccc cattgccgac tccgcgcggg
     1381 caatcctcga cggtcacatt gtgctgtctc gccgactggc ggaagccggg cactatccgg
     1441 ctatcgatat tgaagcgtcg atcagccgcg caatgacggc gttgatcagt gagcaacatt
     1501 acgcgcgagt gcgcaccttc aaacagctgt tgtcgagttt tcagcgtaac cgcgatctgg
     1561 ttagcttcgg cgcgtatgcc aaaggcagtg atccgatgct cgataaagcc atcgccctgt
     1621 ggccgcaact ggagggctat ttacaacaag gcatttttga acgtgcggac tgggaagcgt
     1681 ctctccaggg gctggagcgt attttcccga cagtgtcata acccaggaga taacggcaga
     1741 tggcagaaca tggtgcgctg gcgaccctga aagatctggc agaaaaagag gtagaggatg
     1801 ccgcgcgcct gctgggtgaa atgcgtcgcg ggtgtcagca ggcggaagaa cagctcaaaa
     1861 tgctgatcga ttatcagaat gaatatcgca ataacctcaa cagcgatatg agtgccggga
     1921 tgaccagcaa ccgctggatc aactatcagc agtttatcca gacgctggaa aaagccatta
     1981 ctcagcatcg ccagcaactt aatcagtgga cgcagaaagt tgacattgcc ctgaacagct
     2041 ggcgagaaaa gaaacaacgt ttgcaggcct ggcagacact gcaggaacgg caatccacgg
     2101 cggcactgct tgcagaaaac cgcctcgatc agaaaaagat ggatgagttc gcccagcgcg
     2161 ccgccatgag gaaacctgaa tgattcgctt agcgccctta attaccgccg acgttgacac
     2221 caccacattg cctggcggca aagccagcga tgctgcacaa gattttctcg cgttgttgag
     2281 cgaagcatta gcaggcgaga caactaccga caaagcggcc ccccagttgc tggtggcaac
     2341 agataagccc acgacaaaag gcgagccgct ggtcagcgag attcttgccg atgcgcaaca
     2401 agcggattta ctgatccctg tggatgaaac accgcctgtc atcaacgacg aacaatccac
     2461 atcaacacca ttaaccaccg ctcaaacgat gacgttggct gcggtggctg gcaacaatac
     2521 ggcaaaagac gaaaaagcgg atgatctgaa tgaagacgtc accgcaagcc tgagcgccct
     2581 ttttgcgatg ttgccgggtt ttgacaatac gcccaaagtg actgatgcgc cgtcaaccgt
     2641 gttaccgaca gagaaaccaa cgctcttcac aaaactgact tctgagcaac tcacaacagc
     2701 acagcctgat gacgcccccg gcacaccagc tcagccatta acaccgctgg tagcagaagc
     2761 ccagagtaaa gcggaagtca tcagcacacc ttcaccggtg accgctgccg ccagcccgct
     2821 aatcactcca caccagacac agccactgcc caccgtcgcc gcacctgttt tgagtgcacc
     2881 gctgggttct cacgaatggc aacaatcatt aagccagcat atttcgctgt tcacccgcca
     2941 ggggcaacaa agtgcagagt tgcgactgca cccacaggat ttaggtgaag tgcaaatctc
     3001 cctcaaagtg gatgataacc aggctcaaat ccagatggtt tcaccgcatc agcatgtacg
     3061 cgccgccctg gaagcagcgc tgccggtact gcgtacgcag ctggccgaaa gtggcattca
     3121 gttagggcaa agcaacatca gtggcgaaag ctttagtggt cagcagcagg ccgcttccca
     3181 acaacagcaa agccaacgca cagtaaacca tgaacctctg gcgggggaag acgacgatac
     3241 gcttccggtt cccgtctctt tacaagggcg cgtaacaggc aacagcggcg ttgatatttt
     3301 cgcctaacgt cagaggtagc accgtaatcc gcgtcttttc cccgctttgt tgcgctcaag
     3361 acgcaggata attagccgat aagcagtagc gacacaggaa gaccgcaaca catgactgat
     3421 tacgcgataa gcaagaaaag caagcgatcg ctttggatcc cgattctggt attcattacc
     3481 ctcgcggcct gtgccagcgc aggttacagc tactggcatt cgcatcaggt tgccgctgac
     3541 gacaaagcgc agcaacgcgt cgtgccctca cctgtcttct acgcgctgga taccttcacg
     3601 gtcaatttgg gcgatgcgga tcgcgtgctt tatatcggca taaccctgcg cctgaaagat
     3661 gaagctaccc gctcgcggct gagtgagtat ttgccggaag tccgtagccg tttgctgtta
     3721 ctgttttcgc gtcaggatgc tgccgtactg gcgacagaag aaggcaagaa aaacctgatt
     3781 gccgagatta aaaccacact ttccaccccg cttgttgccg ggcaaccgaa acaggatgtc
     3841 accgacgtgc tgtataccgc ttttattctg cgataacgac atgggcgata gtattctttc
     3901 tcaagctgaa attgatgcgc tgttgaatgg cgacagcgaa gtcaaagacg aaccgacagc
     3961 cagtgttagc ggcgaaagtg acattcgtcc gtacgatccg aatacccaac gacgggttgt
     4021 gcgcgaacgt ttgcaggcgc tggaaatcat taatgagcgc tttgcccgcc attttcgtat
     4081 ggggctgttc aacttgctgc gccgtagccc ggacattacc gtgggggcca tccgcattca
     4141 gccgtaccat gaatttgccc gcaacctgcc ggtgccgacc aacctgaacc ttatccatct
     4201 gaaaccgctg cgcggcacag ggctggtggt gttctcgccg agtctggtgt ttatcgccgt
     4261 ggataacctg tttggcggcg atggacgctt cccgaccaaa gtggaaggtc gcgagtttac
     4321 ccataccgaa cagcgcgtca tcaaccgcat gttgaaactg gcgctagaag gctatagcga
     4381 cgcatggaag gcgattaatc cgctggaagt tgagtacgtg cgttcggaaa tgcaggtgaa
     4441 atttaccaat atcaccacct cgccgaacga cattgtggtt aacacgccgt tccatgtgga
     4501 gattggcaac ctgaccggcg aatttaatat ctgcctgcca ttcagcatga tcgagccgct
     4561 acgggaattg ttggttaacc cgccgctgga aaactcgcgt aatgaagatc agaactggcg
     4621 cgataacctg gtgcgccagg tgcagcattc acagctggag ctggtcgcca actttgccga
     4681 tatctcgcta cgcctgtcgc agattttaaa actgaaaccc ggcgacgtcc tgccgataga
     4741 aaaacccgat cgcatcatcg cccatgttga cggcgtcccg gtgctgacca gtcagtacgg
     4801 caccctcaac ggtcagtatg cgttacggat agaacatttg attaacccga ttttaaattc
     4861 tctgaacgag gaacagccca aatgagtgac atgaataatc cggccgatga caacaacggc
     4921 gcaatggacg atctgtgggc tgaagcgttg agcgaacaaa aatcaaccaa cagcaaaagc
     4981 gctgccgacg cggtgttcca gcaatttggc ggtggtgatg tcagcggaac gttgcaggat
     5041 atcgacctga ttatggatat tccggtcaag ctgaccgtcg agctgggccg tacgcggatg
     5101 accatcaaag agctgttgcg tctgacgcaa gggtccgtcg tggcgctgga cggtctggcg
     5161 ggcgaaccac tggatattct gatcaacggt tatttaatcg cccagggcga agttgtcgta
     5221 gtcgctgata aatatggcgt gcggatcacc gatattatta ccccgtctga gcgaatgcgt
     5281 cgcctgagcc gttagtgatg aataaccacg ctactgtgca atcttccgcg ccggtttctg
     5341 ctgcgccact gctgcaggtg agcggcgcac tcatcgccat tattgccctg atcctcgctg
     5401 ctgcctggct ggtaaaacgg ttgggatttg cccctaaacg cactggcgtt aacggtctga
     5461 aaattagcgc cagtgcttcg ctgggcgcgc gtgaacgggt tgtggtggtc gatgtggatg
     5521 atgcacggct ggtgctcggc gttaccgcag ggcaaatcaa tctgctgcat aaacttcccc
     5581 cttctgcacc aacggaagag ataccgcaga ccgattttca gtcggtcatg aaaaatttgc
     5641 ttaagcgtag cgggagatcc tgatgcgtcg tttattgtct gtcgcacctg tccttctctg
     5701 gctgattacg cccctcgcct tcgcgcaact accgggtatc accagccagc cgctgcctgg
     5761 cggtggacaa agctggtcgc tcccggtgca aacgctggtg ttcatcacct cgttgacgtt
     5821 tattccggca attttactga tgatgaccag tttcacccgc atcatcattg tttttggttt
     5881 attgcgtaac gcgctgggaa caccctccgc gccacctaac caggtattgc tggggctggc
     5941 actgtttttg acctttttta ttatgtcacc ggtgatcgac aaaatttatg tcgatgcgta
     6001 ccagccattc agcgaagaga aaatatcaat gcaggaggcg ctggaaaaag gggcgcagcc
     6061 gctgcgtgag tttatgctgc gtcagacccg tgaggcggat ttagggttgt ttgccagact
     6121 ggcgaatacc ggcccgttgc agggacctga agccgtgccg atgcgcattt tgctcccggc
     6181 ctacgtgacc agcgagttga aaaccgcatt tcagataggc ttcacgattt tcatcccttt
     6241 tttgattatc gacctggtga tagccagcgt gttgatggca ttagggatga tgatggttcc
     6301 ccccgccacc attgctctgc cctttaaact gatgctgttt gtactggtgg atggctggca
     6361 attgctggtc ggttcgctgg cgcagagctt ttacagctag agaggcaaaa tgacacctga
     6421 atcggtcatg atgatgggga ctgaagcgat gaaagtcgcg ctggcactgg ctgccccgct
     6481 gttgctggtg gcgctggtca cgggccttat catcagtatt ttgcaggccg ccacccagat
     6541 caacgaaatg acgctgtcgt ttattccaaa aatcatcgcc gtatttattg ccattattat
     6601 tgctggaccg tggatgctca atctgttgct ggattacgtc cgcaccttgt tcactaacct
     6661 gccgtatatc atcgggtagc ccgtacaatg atgcaggtga caagcgatca atggctttcc
     6721 tggctaagtc tgtacttctg gccgttactg cgcgtgctgg cgctcatttc taccgcgcca
     6781 attctgagcg aacgcagcgt gccgaaacgg gtaaaactgg gtctggcaat gatgatcacg
     6841 ttcgctattg ccccatcatt acctgccaac gatgttcctg ttttttcgtt ctttgctctg
     6901 tggctggccg tgcagcagat cctgatcggc attgcgcttg gttttaccat gcaatttgcc
     6961 tttgccgctg tgcgaaccgc tggcgaaatt atcggtctgc aaatggggct gtcatttgcg
     7021 acgtttgtcg atccggccag ccatctcaat atgcccgttc tagcccgtat catggatatg
     7081 ctggcgttat tgctgttcct gacatttaac ggtcatttat ggttgatttc actgctggtc
     7141 gatacctttc acaccctgcc aattggtggc gaaccgttga acagcaatgc gtttctggca
     7201 ctcaccaaag cagggagttt gattttcctt aacgggctaa tgctggcgtt accgctcatt
     7261 actctgctgc tgacactgaa tctggcatta ggtttactta atcgtatggc cccgcaatta
     7321 tccatttttg ttattggatt tccattaact ctgactgtcg gtatctcttt aatggcggca
     7381 ttaatgccgt taattgcacc tttttgcgaa catttattca gtgagatgtt taatttgctg
     7441 gctgatatta ttagtgaatt gccattaata taattccgta acgtttatca tgttatccta
     7501 aggattatcc gaaaaataat acctacgaac atcttctagg atactcctgc ggcgaaatat
     7561 ttgttataag ctcactcaca tatcgcaaca tttactttac tttaagacaa ttccaggcaa
     7621 attatacaac actttacggg atagtaagtc cgcctgaaaa atcgcgagag tggcgcatta
     7681 ggtgacccat gttgttccgt ttagtcatga tgaaatattc aggtaagggg aattatcgtt
     7741 acgcattgag tgagggtatg ccatgtcaac gattattatg gatttatgta gttacacccg
     7801 actaggttta accgggtatc tgttgagtag aggggttaaa aaaagagaaa tcaacgacat
     7861 tgaaaccgtt gatgaccttg ccatagcttg tgattcacag cgcccttcag tggtgtttat
     7921 taatgaggac tgtttcatcc atgatgcttc taacagtcag cacatcaagc acatcattaa
     7981 tcaacatccc aatacgttat ttatcgtttt tatggcaatt gccaatgttc attttgatga
     8041 atatctattg gtcagaaaaa atttattgat aagttctaaa tcgattaaac cggaatctct
     8101 cgacgatatc cttggcgata ttctgaaaaa agagacaacg ataacctcgt ttttaaatat
     8161 gccgacgtta tcattgagcc gaaccgaatc gagtatgttg cgaatgtgga tggcaggtca
     8221 gggaaccatt caaatctctg accaaatgaa tatcaaagcc aagaccgttt catcgcataa
     8281 aggtaatatt aaacgtaaga tcaaaacgca taataaacag gttatctacc atgtcgtccg
     8341 actgacggat aatgtgacta atggtatttt tgtcaacatg cgctaacaca ttctgactgg
     8401 tggtttccca ccagtcaggc tgaattagat tactctgctt tctccacaaa gataccgtcc
     8461 tgatgccctg cttcattaaa gaaccagatg ccgagcgggt agtcttccag cgaaaccagg
     8521 tacattgtgc cttcactaaa ctcctcaact gccagtacca cgccaggacg acgcggaccg
     8581 ccatccgttt tgactgttac ccgatcattc accttcatcg ttttcctcct gtggctttgt
     8641 gccagtgtag aacaatttcg tttttctggc agcgccaggc gcgcgcgagt gctgattttc
     8701 tcgacggtct atacttaaga gatgccaggc ggacttaacg actggcggca acaacagagt
     8761 aacggttgcg aggaaagatg atgaaaaccg caaaagagta cagcgatacc gcaaaacgtg
     8821 aggtcagcgt cgatgtcgat gccctgctgg cggcgatcaa tgaaattagc gaaagcgaag
     8881 ttcatcgcag ccagaacgat tctgaacacg ttagcgtcga tggacgtgaa tatcatacat
     8941 ggcgtgaatt ggcggatgcc ttcgaactgg atattcatga cttcagcgtc tctgaagtga
     9001 atcgttgaat gcacaataaa aaaatcccga ccctgagggg tcgggatgaa acttgcttaa
     9061 gcaagaagca cttaaaaaat tcgttacacc aggaaatctg atgtgttcat caccttatcc
     9121 gcaatttttt tcgctgacaa gaaaatattc gcgatatgaa tgattaacgc tacttatgaa
     9181 tagaaatgtg acgtgcgtca ccccttggtg gctctggcgt caacgggatc gctcccgaaa
     9241 aaatcatctt tgattatttg tgctgttttg agaatcatcc tggtaagggg agaactgcgc
     9301 gaccatgttt tcaattcaac aaccactact ggtttttagc gatcttgatg gcaccctgct
     9361 ggacagtcat agttatgact ggcaaccggc tgccccctgg ctcagccgtt tacgcgaagg
     9421 gaatgttccc gtcattctct gtagcagtaa aacttcagcg gaaatgctgt acttgcaaaa
     9481 aacgttgggg ctacaagggt taccgctgat tgccgaaaat ggcgcagtga tccagcttgc
     9541 tgaacaatgg caggatatag acggttttcc acgcatcatc tcaggtatta gccatggcga
     9601 aatcagccag gttttaaata cgctacgcga gaaagaacat tttaaattca cgactttcga
     9661 tgatgtcgac gatgcaacca tcgccgaatg gacgggatta agccgtagcc aggcggcgct
     9721 gacgcagctg catgaggcgt cggtaacgct aatctggcgc gacagtgacg agcgtatggc
     9781 acaatttacc gctcgtctga acgaactggg cttacagttt atgcagggtg cgcgcttctg
     9841 gcacgtcctg gatgcctctg ccggaaaaga tcaggccgcc aactggatta tcgcgaccta
     9901 tcaacaatta tcaggcaaac gcccaaccac acttggcctg ggcgatgggc caaacgatgc
     9961 gcccttactg gaggtaatgg attacgcggt gattgtgaaa gggctaaacc gtgaaggggt
    10021 gcatctgcat gatgaggatc cgacccgcgt ctggcgaacg cagcgtgaag gaccggaagg
    10081 ctggcgtgaa gggctggacc attttttctc cgctcgttaa gcgttatcgc tcgcgcatac
    10141 ccgattacgc ccggcctgtt tagcgagata aagccgacgg tcagccagtg actggagttg
    10201 ttcaaaatca taatcaccgg tttcctcgct gctactaacc cccaacgagg cactgatgcg
    10261 tatcgtcgta ctcttagcaa tcagcatctc tttttcattt aacttcaggc gaatacgttc
    10321 tgcgacttcc gcagcctccg tcagactcgc gcctggcaga atcacacaaa actcctcacc
    10381 accgacccgc ccggcaacgt cctgcgcacg caaggaactg ctaattaatc cggcagcatg
    10441 agaaagaaca cggtcgcccg cctgatgacc aaagcggtca ttaatcgctt taaaatggtc
    10501 aagatcgacc tgaatgacag aaaaaggatg ttggtgcgtc tgacacaatt tcgcgagcgg
    10561 acgggctttt tcaaacagtg cgccacgatt atataaacgg gttaaggtgt cgtgccacgc
    10621 ctgccactgc aacgagcttt gcagaacata catgttgcta accatccggc gaatcacata
    10681 ccaggagatg agtaacatgg tggtaaagag cgcccacagc agggttaatg caatgctgat
    10741 actaccgaaa tcgccgcgca cgccttcgct tagcgtatgg acgcgcacca gcacaccgtc
    10801 gaaatggtcc agacgttccc agctaacata gcgactgtcc atacgaatgc cgccccgcgt
    10861 gtcatgttcc atcgcctgcg ccagcaaggc cagttcacga ggatcaaaaa tattccctgt
    10921 tggatggtca ggattggaag aggtcaaaaa tctcagctta ctgtcataga gctgatactc
    10981 accatcgagg tttttatcga tggcgtttct taaaaattgc tgcatagtac gcacaggaat
    11041 actcatcccc agcacgccat accagtagtt attactgtct accggaacac tgacggtaac
    11101 ctgcggttca gtattgctgg cgtgttccgg ttgcgaagta aaccagcgta ccgcgcggtg
    11161 acgattttct cgttgcgaat ggccgataaa ccagggttgg gtgacatagc cgtaataacg
    11221 cgttggaaca ttgcgcgtaa acaaggtcgg ctgcgtcgaa acgtaaaatc cggcacgcga
    11281 gacatacatc gcctgttcaa ccatcgacga ggagttgtgc gccagtcgca gcaagtaacc
    11341 aacttccagt gcagcggtaa tttcattgtc gaggctttca ttttcgcgag acaggagatt
    11401 cccctcgctg actaatgcat ccgacacacc attaactggc agagtgcgcc gtcggttgag
    11461 ttcaatttgc caggcgtgct cgtcgcgatg ctgttcgaac tcggttacag cattacgcag
    11521 tgaagtgaaa tccagtagcg ctacgagagc ttcgcgcatg ccattacgca aaaagatcag
    11581 tttgtcgacg ttatactgca aatgcttatc gagcgcgttg gctacgtttt ccagatgatt
    11641 acgctggctg gagatatagg catcttccag caccactact tcacgccagg tgagcaaggt
    11701 ggaaaaaagc aataccacga taaagcagag attaacgaca tgaccaggcc ccaggcggcg
    11761 tgcgagtttt ttcaaccagc tctggttttc catttttgtc tcgtgctgca ccctgacgac
    11821 tcccaacctg cttttttatg attctggcat aacctggcga tagcgtaccg caaaacagcg
    11881 ataattttat aatggtggca tgataagacg cgactatcat tcattgcagg cattggcaca
    11941 ccaggccgga tgtgatccgg cctggtgtta atgataccct ttcaaacttc ttccgcagaa
    12001 cgactcccct cccccggaac aagctcggtt tcatgaaaag cttcccgctt gaccccgtaa
    12061 ccgtcatacc aacgacactc aaccataccg ctggagtatc cggtgacaat catccgcggg
    12121 ccgccctctt taaccgtaac ttcctcacta accataaagc tcatactcgc ctcctttttt
    12181 cgagtgaaac ttgttcacct tagttgaaga tggcgaattt tgcataatag cctgtgcgag
    12241 tattaatgtg cctgaccgcg tattttcgct acggctttca ctcccagcac cacgatgccg
    12301 ccgatgataa atccaagaat cagatttaaa acagtcggta atatcatcgc caccactgca
    12361 ctttgctgcc cggcgaaatg ttcaatggcg tgatgcagcg gcgcaattcc atgtaccaca
    12421 atcccaccgc cgacgaggaa catcgccagc gtgccgacaa tcgataacgc cttcatcaac
    12481 cagggcgcaa tgatcaataa ccctttacct aatgcctgca tcagcgcgct ggatttttcc
    12541 gccagccaat accccaggtc atcaatctta acgataaccc ctaccagacc gtagacgccc
    12601 acggtcacta ccagcgcgat gcctgaaagc accagcacct gattaagcaa cggcgcttcg
    12661 gccacaatcc ccagcgtgat ggcgacgatt tcggcagaca atataaaatc ggtacgaatc
    12721 gcccctttta ttttgtcctt ttcaaacttc agcggatcct gcgccgccag cttctccaga
    12781 cgctgctggc tctgcgccgg atcttcttta tgtttacgcg cctccagcat atgcagcact
    12841 ttctctactc cttcaaagca gagaaacgcg ccaccaatca tcaacagagg cgtaatcgcc
    12901 cacgggataa acgcactgat gatcagcgcc agcggcacca gaatcacttt attaatcagc
    12961 gatcctttcg ccacgcccca gaccacggga agttcccggt tggcccgcac gcctgaaact
    13021 tgttgcgcat tgagcgataa gtcatcccct aatacaccgg cggttttctt cgccgccagt
    13081 ttgcccatca cggagatatc gtccagtagt gtggcgatat catcgagcaa cgtcagtaaa
    13141 ctactcccgg ccaaaagcat tccctctcat ttttattgtt gaagggtgta agtatgaagc
    13201 aaaaacggca gctctgatac ttgcttcaca ggtcaacatt tttttaacaa ttaagtgcaa
    13261 ttatatctct cgccagcaga atgattttca cgtttactat gaaccacctt tttatttcca
    13321 ccgtgaggga ttatgcgttt ccgccagttg ttaccgcttt ttggcgcgct gtttgcgttg
    13381 tatatcattt ggggctcaac ctattttgtc attcggattg gcgtggaaag ctggcctccg
    13441 ttaatgatgg caggcgttcg attcctggca gccggtattt tattgatggc atttttgcta
    13501 ctgcgcggac acaagctccc cccgctacgt ccgctgctca atgccgcgct gattggcctg
    13561 ttattgctgg ctgtcggtaa tggcatggtg acggttgccg agcatcaaaa tgttccttcc
    13621 ggcatcgccg ccgttgtggt tgcaaccgtg cccctcttta ccctgtgctt cagccgcctg
    13681 tttggcatta aaacgcgcaa actggaatgg gtgggtatcg ccattgggct tgccggaatc
    13741 atcatgctca atagcggtgg aaatttaagc ggcaatccgt ggggcgcgat tctgatttta
    13801 atcggctcga ttagctgggc gtttggctcg gtttatggct cgcgcattac cttacctgta
    13861 gggatgatgg cgggtgctat tgagatgctg gcggcaggcg tggtgttaat gatcgcgtcg
    13921 atgattgcag gtgaaaaact gacggcgctc ccttcccttt caggcttcct tgcggtcggc
    13981 tatctggcgc tgtttggttc gattatcgcc atcaacgctt atatgtattt aatccgtaat
    14041 gtcagtccgg ctctcgccac cagctacgcc tacgttaacc cggtggtcgc ggtcttgctg
    14101 ggtacgggac tgggcggaga gacgctgtcg aagattgaat ggctggcgct cggcgtaatt
    14161 gtcttcgcgg tggtgctggt cacgttggga aaatatctct ttccggcaaa acccgtagtt
    14221 gcgccagtta ttcaggacgc atcaagcgag taaatgaatc ccctgcgtgt cgatctgcgc
    14281 gctggcacct tcgccgcaga tccactcttc cagacgctcg ctaagcgctt catccgtcag
    14341 cttctcgcgc ccacgtaacg cgcactccca gacaatcaat acgcgccagc cgagttcctg
    14401 caagcgactg atatcgcggc gatcgcgctc aacattttta cctatcttct ccagccagaa
    14461 ctcggttcga gtcgcaggca ctttaaacag atagcagtga tgatgatgcc agaagcagcc
    14521 gtgggtaaat atcacgcagc gatattcatc aacgacaaaa tccgggcgtc cgggcagact
    14581 ggcgtcctga acgcgaaacg ccaggccctg cccggttaac agactggcga ggcgcttctc
    14641 tatcgccgta tcacgcgtgg caatcgcgcg catatttttg ctgcgagtgg ccttatcgtg
    14701 aacgtcggcc atgttgtgcc tcttgctgac gcaacgccac cgcctgtttg atttttggct
    14761 caagcagttt tgccaccgcg gcaaagaccg gcacgaccac cgagttaccg aactggcgat
    14821 aggcctgagt gtccgaaacc ggaatgcgga atttcgcttc tcccggcgct tcaaaaccca
    14881 ttaagcgcgc gcattcacga ggcgttaacc gacgtggacg atgttgctga ttctgcggat
    14941 cgtcaaagtc tttctcaccc gtggccatat cccagccacg atcgattaaa atttccgcgc
    15001 catctttgta ataacgcgca gacagcgtac gggtgacgct ttgcggattg ttcggataaa
    15061 ccattccata accgaagccg ttaccgcgcg cctgatgttt tttcgcatat cgatagaggt
    15121 acttccacag caccggcgtc aggatatatt tcgcctcgac catcgggtcc agcagctgcg
    15181 ccagcgtcac tcgctgcgca gggaaacatt cgctgatatc acgcagggta aaatcggctt
    15241 tcagattcag atcgcgacga aaacccacca gcacgatgcg ttcacggtgc tgcggcagaa
    15301 aatgtttgcc gtcgatgatt ttcggatcgt ctggcccgtt atcttctgca tcagccacgt
    15361 catagcccag ttcgtccagc gtctgcatga tgatgcggaa cgttttaccc tggtcgtgac
    15421 ttttcaggtt tttgacgttt tcgagcacaa acatcgccgg acgacgcgcg tcgataatgc
    15481 gtaccacatc aaaaaacagc gtgccctggg tatcgcaggc aaaaccgtgc gcccgcccga
    15541 gcgagttctt tttcgatacg ccagccagcg aaaatggctg acaagggaaa ccggccagta
    15601 aaacatcgtg ttcaggaatg tgttgacgaa tatgttccgc cgccgcctca tcactcacgc
    15661 cttctttatg gctgagggtg atatcgcgga tatcttcatt aaaatgatgc gtcgccggat
    15721 cgcaataatg gttggcttta taagtgcgta ccgcatgttt gttccattcg ctggtaaaca
    15781 cgcattgtcc gccaatcgat tcaaaaccgc gacggatgcc gccaattccg gcaaatagat
    15841 cgataaagcg aaacgcataa tgcggatgat gtgccggtgg tttgggtaac aacgtttgca
    15901 gatgggcgaa ctctttctca cttaaacggt gccatgccga gtcattcgcc agcgcacgtt
    15961 ttaaaattgc cgcgctccag tgattctcac ccacaccatt aagctgcgcc accagcgttt
    16021 taacatcata aatttgcagc agtttctcca gcattgcctg tgcggcattc ccggtgctgt
    16081 atgaatcggt tactgatata ttttcctgca tagatttaac cggccatctg agatggccga
    16141 caggttaaca taattacagc catttagggc cacgcgctta tcccgcgggt gaaaacgcat
    16201 ctatcacctt gtgatcgaca ccttcattct cccccgccag ttcggcactg agcttcgcca
    16261 taaactcgac taaaaaatgc gcgttgtgct gcgccagctg cttgcctctt gcggtttgca
    16321 tggtttgcgg tagcttcagc aacttagtct ggaaatgatc cagcgcgtag cgtttatcat
    16381 caagcgggcg atgctgtgcg aacggatctt caccatcaaa cagcgccacg cccaatgccc
    16441 ctgaaacggc aaacacgcgc gccaggccaa tcgcccccag ggcttccagc cgatccgcat
    16501 cctggacgat tttagcttcc gttgttaagg gggcgatttg cgcgctgaaa ctgtgagcgg
    16561 caatggcatg acaaacggcc tcgattttct ccgccggaaa ttgctcgaac tcttcacgga
    16621 gcaggcgacg cgtctcttct gccgccagga ttgaagaacg ctgcctttgc ggatgatttt
    16681 tcgccaggct cacaatatcg tgaaaataac aggcggttaa aatcaccagc atatcgacat
    16741 cgtcatctgc cgccagcttc tgtgccgtag cccagacgcg g
//
LOCUS       DAMZFT010000109        16662 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.109, whole genome shotgun sequence.
ACCESSION   DAMZFT010000109 DAMZFT010000000
VERSION     DAMZFT010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16662)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16662)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16662
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.109"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(<1..581)
                     /locus_tag="PN977_003670"
     CDS             complement(<1..581)
                     /locus_tag="PN977_003670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HDI6043669.1"
                     /translation="MDVSRRQFFKICAGGMAGTTVAALGFAPKQALAQARNYKLLRAK
                     EIRNTCTYCSVGCGLLMYSLGDGAKNAREAIYHIEGDPDHPVSRGALCPKGAGLLDYV
                     NSENRLRYPEYRAPGSDKWQRISWDEAFSRIAKLMKADRDANFIEKNEQGVTVNRWLS
                     TGMLCASGASNETGMLTQKFARSLGMLAVDNQAR"
     gene            813..1694
                     /gene="yddG"
                     /locus_tag="PN977_003671"
     CDS             813..1694
                     /gene="yddG"
                     /locus_tag="PN977_003671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415990.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid efflux DMT transporter YddG"
                     /protein_id="HDI6043670.1"
                     /translation="MTRQKATLIGLIAIVLWSTMVGLIRGVSEGLGPVGGAAAIYSLS
                     GLLLIFTVGFPRIRQIPKGYLLAGCLLFVSYEICLALSLGYAATRHQAIEVGMVNYLW
                     PSLTILFAILFNGQKTNWLIVPGLLLALVGVCWVLGGDNGLHYDEIINNITTSPLSYF
                     LAFIGAFIWAAYCTVTNKYARGFNGITVFVLLTGASLWVYYFLTPQPEMVFSTPVMIK
                     LISAAFTLGFAYAAWNVGILHGNVTIMAVGSYFTPVLSSALAAVLLSAPLSFSFWQGA
                     LMVCGGSLLCWLATRRG"
     gene            1952..3052
                     /gene="ompC"
                     /locus_tag="PN977_003672"
     CDS             1952..3052
                     /gene="ompC"
                     /locus_tag="PN977_003672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HDI6043671.1"
                     /translation="MKLKIVAVVVTGLLAANVAHAAEVYNKDGNKLDLYGKVTALRYF
                     TDDKRDDGDKTYARLGFKGETQINDQMIGFGHWEYDFKGYNDEANGSRGNKTRLAYAG
                     LKISEFGSLDYGRNYGVGYDIGSWTDMLPEFGGDTWSQKDVFMTYRTTGVATYRNYDF
                     FGLIEGLNFAAQYQGKNERTDNGHLYGADYTRANGDGFGISSTYVYDGFGIGAVYTKS
                     DRTNAQERAAANPLNASGKNAELWATGIKYDANNIYFAANYAETLNMTTYGDGYISNK
                     AQSFEVVAQYQFDFGLRPSLAYLKSKGRDLGRYGDQDMIEYIDVGATYFFNKNMSTYV
                     DYKINLIDESDFTRAVDIRTDNIVATGITYQF"
     gene            3813..4481
                     /locus_tag="PN977_003673"
     CDS             3813..4481
                     /locus_tag="PN977_003673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019843110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine Rich Repeat family protein"
                     /protein_id="HDI6043672.1"
                     /translation="MINDLILHNHPIVEKITLNDNHIAHLNAKTTTKLEYLNLSNNNL
                     LPTDDIDQLISSKHLWHVLVNGINNDPLAQMQYWTAVRNIIDDTNEVTIDLSYNLAIT
                     NIDTSDEHLVEVSENSEGNHIKENDSMSIRYRSKYYSREYALIEEETIFSDAELKAIL
                     PMRRMYGVGDYKSNSSSLPSHSGLKDPTGTPVCYYIHNEDKPSLGFGPTSNNWLSQSF
                     TTEL"
     gene            4544..5800
                     /locus_tag="PN977_003674"
     CDS             4544..5800
                     /locus_tag="PN977_003674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000671707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HDI6043673.1"
                     /translation="MKFPLIFNKINPQSIQQHPEKNELNWMLELNQWKAERILTGEIH
                     RPECRNEAAKRINCAFLSKQNDIDLSGLNLTTQPPGLQNFTSINLDNNQLTHFDTTTY
                     DRLVKLSLNSNALESINFPQGRNVSITHISMNNNSLRNIDIDRLSSITYFSAAHNQLE
                     FVQLECCERLQYLNLSHNQLTDIVAENKDELLLLDLSHNKLTSLHNALFPNLNTLLIN
                     NNLLSEIKIFFSNFCNVQTLNAANNQLEKINLHFLTYLSSIKSLRLDNNKITRIDTKN
                     TSDIGTLFPIIKQSKNLNFLNVSGKNNCPTMQLMLFNLFSPALKLNTGLAILSPGAFE
                     VHSDGVDVDNELFHYTIKEAYTPYNIHTYKTEEVVNQMNIKIKNMTLDEINNTYCNND
                     YYNEAIREEPIDFLGRSFSSSSWPFQ"
     gene            6110..7498
                     /gene="narU"
                     /locus_tag="PN977_003675"
     CDS             6110..7498
                     /gene="narU"
                     /locus_tag="PN977_003675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite transporter NarU"
                     /protein_id="HDI6043674.1"
                     /translation="MALQNEKNSRYLLRDWKPENPAFWENKGKHIARRNLWISVSCLL
                     LAFCVWMLFSAVTVNLNKIGFNFTTDQLFLLTALPSVSGALLRVPYSFMVPIFGGRRW
                     TVFSTAILIIPCVWLGIAVQNPNTPFGIFIVIALLCGFAGANFASSMGNISFFFPKAK
                     QGSALGINGGLGNLGVSVMQLVAPLVIFVPVFAFLGVNGVPQVDGSVMSLANAAWIWV
                     PLLAIATIAAWSGMNDIASSRASIADQLPVLQRLHLWLLSLLYLATFGSFIGFSAGFA
                     MLAKTQFPDVNILRLAFFGPFIGAIARSLGGAISDKFGGVRVTLINFIFMAIFSALLF
                     LTLPGTGSGNFIAFYAVFMGLFLTAGLGSGSTFQMIAVIFRQITIYRVKMKGGSDEQA
                     QREAVTETAAALGFISAIGAVGGFFIPQAFGMSLNMTGSPVGAMKVFLIFYIVCVLLT
                     WLVYGRRKFSQK"
     gene            7593..11333
                     /gene="narZ"
                     /locus_tag="PN977_003676"
     CDS             7593..11333
                     /gene="narZ"
                     /locus_tag="PN977_003676"
                     /EC_number="1.7.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase Z subunit alpha"
                     /protein_id="HDI6043675.1"
                     /translation="MSKLLDRFRYFKQKGDTFADGHGQVMHSNRDWEDSYRQRWQFDK
                     IVRSTHGVNCTGSCSWKIYVKNGLVTWEIQQTDYPRTRPDLPNHEPRGCPRGASYSWY
                     LYSANRLKYPLIRKRLIELWREALKQHSDPVLAWASIMNDPQKCLSYKQVRGRGGFIR
                     SNWQELNQLIAAANVWTIKTYGPDRVAGFSPIPAMSMVSYAAGTRYLSLLGGTCLSFY
                     DWYCDLPPASPMTWGEQTDVPESADWYNSSYIIAWGSNVPQTRTPDAHFFTEVRYKGT
                     KTIAITPDYSEVAKLCDQWLAPKQGTDSALAMAMGHVILKEFHLDNPSDYFINYCRRY
                     SDMPMLVMLEPRDDGSYVPGRMVRASDLVDGLGESNNPQWKTVAVNTAGELVVPNGSI
                     GFRWGEKGKWNLESIAAGTETELSLTLLGQHDAVAGVAFPYFGGIENPHFRRVKHNPV
                     LVRQLPVKNLTLADGNTCPVVSVYDLVLANYGLDRGLEDENSAKDYAEIKPYTPAWGE
                     QITGVPRQYIETIAREFADTAHKTHGRSMIILGAGVNHWYHMDMNYRGMINMLIFCGC
                     VGQSGGGWAHYVGQEKLRPQTGWLPLAFALDWNRPPRQMNSTSFFYNHSSQWRYEKVS
                     AQELLSPLADASKYSGHLIDFNVRAERMGWLPSAPQLGRNPLGIKAEADKAGLSPTEF
                     TAQALKSGDLRMACEQPDSGSNHPRNLFVWRSNLLGSSGKGHEYMQKYLLGTESGIQG
                     EELGASDGIKPEEVEWQTAAIEGKLDLLVTLDFRMSSTCLFSDIVLPTATWYEKDDMN
                     TSDMHPFIHPLSAAVDPAWESRSDWEIYKGIAKAFSQLCVGHLGKETDVVLQPLLHDS
                     PAELSQPCEVLDWRKGECDLIPGKTAPNIVAVERDYPATYERFTSLGPLMDKLGNGGK
                     GISWNTQDEIDFLGKLNYTKREGPAQGRPLIDTAIDASEVILALAPETNGHVAVKAWQ
                     ALGEITGREHTHLALHKEDEKIRFRDIQAQPRKIISSPTWSGLESDHVSYNAGYTNVH
                     ELIPWRTLSGRQQLYQDHPWMRAFGESLVAYRPPIDTRSVSEMRQIPPNGFPEKALNF
                     LTPHQKWGIHSTYSENLLMLTLSRGGPIVWISETDARELTIVDNDWVEVFNANGALTA
                     RAVVSQRVPPGMTMMYHAQERIMNIPGSEVTGMRGGIHNSVTRVCPKPTHMIGGYAQL
                     AWGFNYYGTVGSNRDEFIMIRKMKNVNWLDDEGRDQVQEAKK"
     gene            11330..12874
                     /gene="narH"
                     /locus_tag="PN977_003677"
     CDS             11330..12874
                     /gene="narH"
                     /locus_tag="PN977_003677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HDI6043676.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTGREGMEYAWFNNV
                     ETKPGIGYPKNWEDQEEWQGGWVRDVNGKIRPRLGSKMGVITKIFANPVVPQIDDYYE
                     PFTFDYEHLHSAPEGKHIPTARPRSLIDGKRMDKVIWGPNWEELLGGEFEKRARDRNF
                     EAMQKEMYGQFENTFMMYLPRLCEHCLNPSCVATCPSGAIYKREEDGIVLIDQDKCRG
                     WRLCISGCPYKKIYFNWKSGKSEKCIFCYPRIESGQPTVCSETCVGRIRYLGVLLYDA
                     DRIEEAASTEREVDLYERQCEVFLDPHDPSVIEEALKQGIPQNVIDAAQRSPVYKMAM
                     DWKLALPLHPEYRTLPMVWYVPPLSPIQSYADAGGLPKSEGVLPAIESLRIPVQYLAN
                     MLSAGDTGPVLRALKRMMAMRHYMRSQTVEGVTDTRAIDEVGLSVAQVEEMYRYLAIA
                     NYEDRFVIPTSHREMAGDAFAERNGCGFTFGDGCHGSDSKFNLFNSSRIDAINITEVR
                     DKAEGE"
     gene            12874..13569
                     /gene="narW"
                     /locus_tag="PN977_003678"
     CDS             12874..13569
                     /gene="narW"
                     /locus_tag="PN977_003678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HDI6043677.1"
                     /translation="MQILKVIGLLMEYPDELLWECKEDALALIRRDAPMLTDFTRNLL
                     NAPLLDKQAEWCEVFDRGRTTSLLLFEHVHAESRDRGQAMVDLLAEYEKVGLQLDCRE
                     LPDYLPLYLEYLSVLPDDQAKEGLLNVAPILALLGGRLKQREAPWYALFDTLLQLAGS
                     TLSSDSVTKQVNSEERDDTRQALDAVWEEEQVKFIEDNATACDSSPLNQYQRRFSQDV
                     APQYVDISAGGGK"
     gene            13566..14246
                     /gene="narI"
                     /locus_tag="PN977_003679"
     CDS             13566..14246
                     /gene="narI"
                     /locus_tag="PN977_003679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HDI6043678.1"
                     /translation="MIQYLNVFFYDIYPYICATVFFLGSWLRYDYGQYTWRASSSQML
                     DKRGMVIWSNLFHIGILGIFFGHLFGMLTPHWMYAWFLPVAVKQQMAMILGGICGVLT
                     LIGGAGLLVRRLTNPRIRATSSGADILILAILLIQCILGLTTIPFSAQHPDGSEMMKL
                     VGWAQSIVTFQGGASTHLDGVAFIFRVHLVLGMTIFLLFPFTRLVHVWSAPFEYFTRR
                     YQIVRSRR"
     gene            14326..15219
                     /locus_tag="PN977_003680"
     CDS             14326..15219
                     /locus_tag="PN977_003680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family isomerase"
                     /protein_id="HDI6043679.1"
                     /translation="MKPQVYHVDAFTSQPFRGNSAGVVFPADNLSEAQMQLIARELGH
                     SETAFLLHSDDSDVRIRYFTPTVEVPICGHATVAAHYVRAKVLGLGNCTVWQTSLAGK
                     HRVTIEKQNDDYRISLEQGTPGFEPPLEGETRAAIINALHLTEDDILQGLPIQVATTG
                     HSKVMIPLKPEVDIDALSPDLAALTTISKQIGCNGFFPFQIRPGKNETDGRMFSPAIG
                     IVEDPVTGNANGPMGAWLVHHNVLPHDGNVLRVKGHQGRALGRDGVIEVTVTIRDNQP
                     EKVTISGTAVILFHAEWAIKL"
     gene            complement(15315..16160)
                     /gene="nhoA"
                     /locus_tag="PN977_003681"
     CDS             complement(15315..16160)
                     /gene="nhoA"
                     /locus_tag="PN977_003681"
                     /EC_number="2.3.1.118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-hydroxyarylamine O-acetyltransferase"
                     /protein_id="HDI6043680.1"
                     /translation="MTPILNHYFARINWSGAAAANIDTLRALHLKHNCTIPFENLDVL
                     LPREIQLDDQSLEEKLVTARRGGYCFEQNGVFERVLRELGFNVRSLLGRVVLSNPPAL
                     PPRTHRLLLVELEGEKWIADVGFGGQTLTAPIRLVPELVQTTPHGEYRLLQEGDDWVL
                     QFNHHQHWQSMYRFDLCEQQQSDYVMGNFWSAHWPQSHFRHHLLMCRHLPDGGKLTLT
                     NFHFTHYENGHAVEQRNLPDVASLYAVMQEQFGLGVDDAKHGFTVDELALVMAAFDTH
                     PEAGK"
     gene            16335..>16662
                     /locus_tag="PN977_003682"
     CDS             16335..>16662
                     /locus_tag="PN977_003682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415979.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HDI6043681.1"
                     /translation="MSRFIPVELHHASRLLNHGPTVMITSFDEQSQRRNIMAAAWSMP
                     VEFEPPRVAIVVDKSTWTRELIERNGKFGIVIPGVAATNWTWAVGSVSGRDEDKFNCY
                     GIPVVKG"
BASE COUNT         4337 a         4092 c         3966 g         4267 t
ORIGIN      
        1 cgcgcctggt tgtctaccgc cagcatcccg agggagcggg caaatttttg agtcagcatc
       61 ccggtttcgt tgctggcacc ggaggcacac agcataccgg tagaaagcca acggtttacc
      121 gttacgccct gctcgttctt ttcaataaag ttagcgtcac ggtcagcttt catcagcttc
      181 gcaatacggg agaatgcttc atcccagcta atacgctgcc atttatcaga acctggcgca
      241 cgatattccg ggtagcgcag acggttttcg ctgttgacgt aatccagcaa accggcccct
      301 ttcgggcaca gcgcaccacg gcttaccgga tgatccgggt caccttcaat gtgataaatc
      361 gcttctctgg cgtttttcgc gccatcaccc aggctataca tcaatagccc gcaacctacg
      421 gaacagtatg tgcaggtgtt acggatctct ttagcgcgta ataatttgta gtttcgcgcc
      481 tgagccagtg cttgctttgg ggcaaagccc aatgccgcta ctgttgttcc agccataccg
      541 cccgcgcaga ttttaaaaaa ttgtctgcga ctgacgtcca ttgctttcct cttttttcag
      601 gggtattact gcgcgaggaa actaacagta aaattctgaa tctttttgcg cgaaatcaaa
      661 aagaacacgt gtttgccaca aaataatccg cctgcgcaaa ttgacctacc tcaatagcgg
      721 tagaaaaacg caccactgcc tgacaggcca gttaaaaaaa tgctataaaa ttcagcttaa
      781 tttttaacgg ctagagagac aaaacagcga gcatgacacg acaaaaagca acgctcatag
      841 ggctaatagc gatcgtcctg tggagcacga tggtaggatt gattcgcggt gtcagtgagg
      901 ggctcggccc ggtcggcggc gcagctgcta tctattcatt aagcgggctg ctgttaatct
      961 tcacggttgg atttccgcgt attcggcaga tcccgaaagg ctatttactc gccgggtgtc
     1021 tgttattcgt cagctatgaa atctgtctgg cgctttcctt aggatatgcg gcgacccgtc
     1081 atcaggcgat tgaagtgggt atggtgaact atctgtggcc cagcctgaca attctctttg
     1141 ccattctgtt taatggtcag aaaaccaact ggctgattgt accaggatta ttattagccc
     1201 tggttggcgt ctgctgggtg ttaggtggtg acaatgggtt acattatgat gaaatcatca
     1261 ataatatcac caccagccca ttgagttatt tcctggcgtt tattggtgcg tttatctggg
     1321 cagcctattg cacagtaacg aataaatacg cacgcggatt taacggaatt accgtttttg
     1381 tcctgctaac gggagcaagt ctgtgggttt actattttct cacgccacaa ccagaaatgg
     1441 tatttagcac gccagtcatg attaaactca tctctgcggc atttacctta ggatttgctt
     1501 atgctgcatg gaacgtcggt atattacatg gcaacgtcac cattatggcg gtaggttcgt
     1561 attttacgcc cgtcctttcc tcagcgcttg cagccgtgct gctcagcgcc ccgctgtcgt
     1621 tctcgttctg gcaaggcgcg ctaatggtct gcggcggttc cctgctctgc tggctggcga
     1681 cacgtcgtgg ctaaaataat tgtcgggtct acggcccggc agttaatcag ataaattccg
     1741 acaatataaa aaataaattt caaataccag tagttcacct tatatttcat ttcgacttcg
     1801 atcacatatc gataaataat attatttcat ctatttttct cattgtgttt aatcaccatt
     1861 ccattttaaa aacagtatgt catttataaa tgacgtcgtt taaaattcgt ttagaaaata
     1921 cgtttaacga taattaaaag gattctgtta aatgaaatta aaaatagttg cggtggttgt
     1981 aactggtttg ttagctgcga acgtagcaca cgctgccgaa gtctataaca aggatggtaa
     2041 taaactcgac ctttatggca aggttaccgc tctacgttat tttactgatg ataagcgtga
     2101 cgatggtgat aaaacttatg cccgtctcgg ctttaaagga gaaacgcaaa tcaatgatca
     2161 aatgattggt tttggtcact gggaatatga ttttaaaggc tataacgatg aagccaacgg
     2221 ctcgcgcggt aacaagaccc gtctggccta tgcaggttta aaaattagtg aatttggctc
     2281 tctggactat ggtcgtaact acggtgtcgg ctatgacatt ggttcatgga ccgatatgtt
     2341 gccagaattt ggtggcgata cctggagtca gaaagatgtc ttcatgacat accgtactac
     2401 cggtgtggca acctatcgca actacgattt cttcggctta attgaaggtc tgaactttgc
     2461 cgcgcaatat caaggcaaaa atgaacgcac tgacaatggt catctttatg gtgctgacta
     2521 cacgcgtgcc aatggtgacg gtttcggtat ctcctcaact tatgtttatg atggctttgg
     2581 tatcggtgcg gtgtatacca aatccgatcg gacaaatgcg caggaaagag ccgctgctaa
     2641 tcctctcaat gcctccggta agaatgcaga actgtgggct acaggtataa aatatgatgc
     2701 caacaacatc tactttgcag ctaattacgc tgaaacatta aacatgacca cctatggcga
     2761 tggttatatc tctaacaaag cacaaagttt tgaagtagtg gcacaatatc aattcgactt
     2821 cggcttgcgc ccatcactcg cttacctgaa atcgaaaggc agagatctgg gccgctacgg
     2881 cgaccaggac atgattgagt atatcgacgt tggtgcgacg tatttcttca acaaaaatat
     2941 gtcgacctat gttgattata aaatcaacct gattgatgaa agcgacttta cccgtgccgt
     3001 agatattcgc accgataaca tcgtcgcaac gggaattacc tatcagttct aacatcctct
     3061 cctgaccagc ccttctctgg gctggttttt gatttaagtg ctgctgtatg actccttaag
     3121 taaaaatgag caatttttga tatatctggt cattcacctc aattaatatt attccatacc
     3181 ctccggggac atcttactat aatatttatt gatgagtgat tatgaccaat attaatacag
     3241 cttgtgtaaa aaataatgcc agttatcaat taaataacgc attacctaac aaagaaacca
     3301 tctccagcaa tttttgtgaa cgactggcac aatggggtaa taagtcgctt aataatggtg
     3361 aagaaagagc aattgccgtg gagcgaatta aagaagctta caattcgaac atggcatctc
     3421 ttgatttgtc atatcttgat ttgagtgagt taccccctat cccctcgaca gttaacacgt
     3481 taaatctgga aaacaattgt ctcacttgtc ttgactttac tgacaatgcc agcctcgtca
     3541 atatcaacct cagctttaat aaaattaaaa cgataacctt tccaaatcaa tcaaaactgg
     3601 aaaatattta tattgatcac aataatctgg aaaatttgga tttaaaaaat cagctttcat
     3661 tggttaacct ggaagcgcaa aataacaacc tgacaaaaat taatatttct gatagttata
     3721 aactgaaatt tcttaatctt gattataaca aactagcatc gctggatctc tcccggcaag
     3781 aatctctgat tgagttaagt gcccatcaca acatgatcaa tgaccttata ttacacaatc
     3841 accccatagt ggagaaaatc actttaaacg acaaccatat tgcacattta aacgcgaaaa
     3901 ccactacaaa actggaatat ttaaacttaa gcaataacaa tttattgcca acagatgaca
     3961 ttgatcaatt aatatcatca aaacatcttt ggcatgtatt agttaacggc atcaacaatg
     4021 atccacttgc ccaaatgcag tactggactg cagtaagaaa tataattgat gacactaatg
     4081 aagtgaccat tgatttatct tataacctgg caatcacaaa tatcgatacc agcgatgaac
     4141 atcttgtaga agtaagcgag aattccgaag gaaatcatat aaaagaaaat gactcaatgt
     4201 ctattcgtta tagatcaaaa tattattcca gagagtacgc cttaatagaa gaagaaacaa
     4261 tattttctga cgcagaacta aaagctattc tgcctatgcg tcgcatgtac ggggttggtg
     4321 actataagtc aaattcctct tctctaccct cacactcggg gctaaaggac ccaacgggca
     4381 cacccgtctg ttattatatt cataatgagg ataaaccttc cttaggtttt ggtccaacat
     4441 ccaataattg gttaagccaa tcctttacaa cagagttata aaacacatcc tgatttatca
     4501 agggtaataa atcattaaaa taaagcatta ctacggagca gttatgaaat tccctttaat
     4561 atttaataaa ataaatccac aatccataca gcaacatcca gaaaaaaatg aacttaactg
     4621 gatgctcgaa ttaaatcaat ggaaagcaga acgcatactt acaggtgaaa tccatcgtcc
     4681 ggaatgccga aacgaagccg ctaaaaggat aaattgtgct tttttgtcga aacagaatga
     4741 tattgattta tcaggactta atttaactac ccaaccacca gggctgcaaa acttcacctc
     4801 tatcaatctt gataataacc aactcacaca ttttgataca accacctacg atagactcgt
     4861 aaagcttagt ctgaatagta atgctcttga gtcaataaat tttcctcaag gtagaaatgt
     4921 aagcattaca catatatcta tgaataataa ttctctcaga aatattgata tagatcggct
     4981 ttcatcaatt acttatttta gtgcggcaca taatcaacta gagtttgtgc aattagaatg
     5041 ttgcgaaagg ctgcagtacc tgaatctcag ccacaatcaa ttaactgata ttgtggcaga
     5101 aaataaagat gaacttttac tactggatct atcccataat aaactaacaa gtttacataa
     5161 tgccttattc cccaacttga atacgttact tatcaacaac aacttgcttt ctgaaattaa
     5221 aatattcttt agcaacttct gcaatgttca gacattaaac gctgcgaaca atcagttgga
     5281 aaaaataaac cttcatttcc tgacttatct ttcatctatc aaaagtttaa ggctggacaa
     5341 taataaaata acccgcattg acactaagaa tacatccgat attggaactt tattccccat
     5401 aataaaacag agcaaaaact taaatttttt aaatgtttct gggaagaaca attgccctac
     5461 tatgcagctc atgttattta atttattttc cccagcactt aagcttaata ctggcctggc
     5521 aattctttcg cctggtgcat ttgaagttca ctctgacgga gtagatgtgg ataacgaatt
     5581 gtttcactat actattaaag aagcatatac cccatataat atacacactt ataaaacaga
     5641 agaagttgta aatcagatga atataaaaat taaaaatatg acattagatg aaataaacaa
     5701 tacttactgt aataacgatt attacaatga ggcgataaga gaggaaccga tagactttct
     5761 gggcagatcg ttttcctcca gctcatggcc ttttcagtga ataaatcatc actaattcta
     5821 taagtgtctg gtcttgttga gaatatagtt aatatgcaac cctttagatt cacgatctga
     5881 agggttgaaa catgacatac gttcaaagat taacccccgt ttaatggtta catttagtaa
     5941 cacatatctg tctgtctaat ccacattctg cccccgcagt tacgctttct tatctattct
     6001 tctaatcagc acttcacctg attcatcctt aattctaata actggctatt gcgccttacc
     6061 tcctgttttg tctttttttc attaccaatg tgtgcatgtg aggaacaata tggcactgca
     6121 aaatgagaaa aatagtcgtt atcttttacg cgactggaaa ccagaaaatc cggccttctg
     6181 ggaaaataaa ggaaaacata ttgcacgaag aaacctctgg atatcagtca gttgtctact
     6241 tcttgccttc tgtgtctgga tgctatttag cgcagttact gttaatctca ataaaatcgg
     6301 ttttaatttc actaccgatc aactcttttt attaaccgca ttaccctccg tttctggcgc
     6361 attattgcgt gtcccctact cctttatggt gcctatattc ggtggacgcc gatggacggt
     6421 ttttagtact gcaatcctga ttattccttg cgtctggctc ggaattgccg tgcagaatcc
     6481 gaatactcct tttgggatat ttatcgttat cgctttgcta tgcggttttg caggtgcgaa
     6541 ctttgcttcg agcatgggca atatcagttt cttcttccca aaagccaaac aagggagcgc
     6601 acttgggatt aatggcggat taggaaactt aggtgtaagt gtgatgcagc tggttgcacc
     6661 gctggtcatt tttgtacccg tatttgcctt tctcggcgtc aatggcgtac cgcaggtcga
     6721 cggttcggta atgtcgctgg cgaatgccgc atggatttgg gtgccattac tggcgattgc
     6781 cacgatcgcc gcctggtcag ggatgaatga tatcgccagt tcacgcgcgt caattgccga
     6841 ccagctgcca gtgttacaac gcctgcatct ctggctgctg agcctgcttt accttgccac
     6901 cttcggttcg tttatcggtt tttctgcggg ttttgccatg ctggcgaaaa ctcagttccc
     6961 ggatgtgaat attctgcgcc tggcgttctt tggcccattt atcggtgcca tcgcgcgatc
     7021 gcttggtggt gctatttccg ataaattcgg cggcgtgcgg gtgacgttga tcaacttcat
     7081 ttttatggcg attttcagcg ccctgctgtt ccttacctta ccgggcacag gctccggtaa
     7141 tttcatcgca ttttacgccg tatttatggg gctgtttctg accgcgggtc tgggaagtgg
     7201 ttctactttc cagatgatcg ccgtcatctt tcgccagata accatttatc gggtgaagat
     7261 gaaaggcggt agtgatgagc aagctcaaag agaagccgtc accgaaacgg cagccgctct
     7321 gggctttatc tcagccattg gcgcagtggg cggctttttt attccgcagg cgtttggcat
     7381 gtcgctcaat atgaccggct ctccggtggg cgcgatgaaa gtgtttttaa tcttctacat
     7441 cgtttgtgtg ctgctgacct ggctggttta tggtcggcgg aagttcagcc aaaaataaga
     7501 gatcgatgcc tgacacgtct tatcaggcta caaccgtgtc gcacccgaca aagcatcaga
     7561 tcccaattca ttcctggagc aggagttatg tcatgagtaa acttttggat cgctttcgct
     7621 acttcaaaca aaagggcgat acctttgccg atggtcacgg acaggtgatg catagcaacc
     7681 gcgactggga ggacagctat cgccagcgtt ggcagttcga caaaatcgtg cgttccaccc
     7741 acggtgttaa ctgtacaggc tcctgtagct ggaaaatcta cgttaaaaat ggtctggtga
     7801 cctgggaaat ccaacagacc gactacccgc gcactcgccc tgacctgcca aatcacgaac
     7861 ctcgcggctg cccgcgtggc gcaagttact cctggtatct ctacagcgct aaccgcctga
     7921 aatacccgct cattcgtaaa cgactgattg aactgtggcg cgaagccctc aagcaacaca
     7981 gcgatccggt actggcgtgg gcatcaatta tgaacgatcc gcaaaagtgc ctgagctaca
     8041 aacaagtacg tggacgcggc ggctttatcc gctccaactg gcaggagcta aaccagctga
     8101 ttgccgctgc taacgtctgg accatcaaaa cttacggccc ggatcgcgtt gccggtttct
     8161 cgccgatccc ggcgatgtcg atggtttctt acgccgccgg aacacgttat ctgtcgctgt
     8221 taggtggcac ctgtttaagc ttctacgact ggtattgcga cctgccgccc gcctcgccga
     8281 tgacctgggg cgagcaaacc gacgtaccgg aatctgccga ctggtataac tccagttaca
     8341 tcatcgcctg ggggtctaac gtaccgcaga cacgtacgcc ggacgcccat ttctttaccg
     8401 aagtacgcta caaaggcact aaaaccatcg ccattacccc tgactactct gaagtggcca
     8461 aattgtgcga ccagtggctg gcaccgaaac aaggcactga tagtgccctg gcaatggcaa
     8521 tgggccatgt cattttaaaa gagtttcatc tcgataatcc cagcgactac tttatcaact
     8581 actgccgccg ctacagcgac atgccgatgc tggtaatgct ggaacctcgc gacgacggca
     8641 gctacgttcc cgggcgcatg gtccgcgcat ctgacctggt ggatggactg ggcgaaagca
     8701 ataatccgca gtggaaaacc gtggcagtta ataccgcagg tgaactggtg gtgccgaacg
     8761 gttcgattgg ttttcgctgg ggagaaaaag gcaaatggaa tctggaatcc attgccgccg
     8821 gtacggaaac cgaattgtcg ttaactctgc tcggtcaaca tgatgccgtt gcaggcgtgg
     8881 ccttccccta ctttggcggc attgaaaatc cgcattttcg ccgcgtaaaa cacaatccgg
     8941 tcctggtgcg ccaattgccc gttaaaaacc tgacattagc cgatggcaac acctgtccgg
     9001 tcgtcagcgt ttatgatttg gtactggcga attatggcct cgatcgcggg ctggaagatg
     9061 aaaacagcgc gaaagattac gctgaaatca aaccgtacac cccagcctgg ggtgagcaaa
     9121 ttaccggcgt gccgcgccag tatattgaaa ccatcgcccg tgaatttgcc gataccgccc
     9181 ataaaacgca tgggcgttcg atgattatcc tcggtgctgg cgtcaaccat tggtatcaca
     9241 tggacatgaa ctaccgtggg atgatcaaca tgctgatctt ctgcggttgt gtcgggcaaa
     9301 gcggtggcgg ctgggcacac tatgtcggtc aggaaaaact gcgcccgcaa accggctggc
     9361 tgcctctggc ctttgcgctc gactggaacc gaccaccgcg ccagatgaac agcacctcgt
     9421 ttttctacaa tcattccagc caatggcgct atgaaaaagt ctctgctcag gagttacttt
     9481 caccgcttgc cgatgccagt aagtacagcg gtcatctgat tgatttcaac gttcgcgccg
     9541 aacgtatggg ctggctacct tctgcgccgc agttggggcg taatccgctc gggattaaag
     9601 ctgaagccga caaggccgga ttatctccca cagaattcac cgcccaggcg ttgaaatcgg
     9661 gcgatttacg tatggcctgc gaacaacccg atagcggcag caatcatccg cgtaatttgt
     9721 ttgtctggcg ttctaacctg cttggttcct ccggcaaagg ccacgagtat atgcagaagt
     9781 atctgctggg gaccgaaagc gggattcagg gcgaggaact cggtgccagc gacgggatca
     9841 aaccggaaga agtcgagtgg caaactgcgg cgattgaagg caagctcgac ctgctggtga
     9901 cgctcgactt ccgtatgtcc agtacctgcc tgttctccga tatcgttctg cccaccgcca
     9961 cctggtacga aaaagacgat atgaacacct cggatatgca tccgtttatt catccgcttt
    10021 ctgcggcggt cgatccggcg tgggaatcac gcagcgactg ggaaatctac aaaggtatcg
    10081 ccaaagcatt ttcgcaactg tgcgtgggtc atcttggcaa agaaaccgac gtggtattac
    10141 aaccactgct acatgactct ccggcagaac tctcccagcc gtgtgaagtg ctcgactggc
    10201 gcaaaggcga atgcgatctg atcccgggta aaaccgcgcc caatattgtg gcggtggaac
    10261 gcgactaccc tgctacctat gaacgcttta cctcgctcgg gccattgatg gacaaacttg
    10321 gcaacggcgg taaagggatt tcgtggaata cccaggatga aatcgatttc ctcggcaaac
    10381 tcaactacac caagcgtgaa ggcccggcac aggggcgtcc gctgattgac accgccattg
    10441 acgcatcaga agtgattctg gcgctggcac cggaaaccaa cggtcatgtt gcagtcaaag
    10501 cgtggcaggc gctgggcgag atcaccggac gcgaacatac ccatctggcg ctgcacaaag
    10561 aggacgagaa gattcgcttt cgcgatattc aggcgcagcc acgtaaaatt atctccagcc
    10621 ccacatggtc cggtctggaa agcgatcacg tttcctataa cgcgggatac accaacgttc
    10681 atgagttaat tccgtggcgc acgctgtcgg gacgccagca gctctatcag gatcatccgt
    10741 ggatgcgtgc ttttggtgaa agcctggtgg cttatcgccc gcctatcgac acccgtagcg
    10801 tcagtgaaat gcgccagata ccgccaaacg gcttcccgga aaaagcactt aacttcctga
    10861 cgccgcacca gaaatggggc attcactcaa cctacagtga aaacctgcta atgctgacgc
    10921 tctctcgcgg tggaccaatt gtctggatca gcgaaaccga tgcccgtgaa ctgaccattg
    10981 tcgataacga ctgggtggaa gtgtttaacg ccaacggcgc gctgactgcc cgcgcggtgg
    11041 tcagccaacg tgtaccgccg ggtatgacca tgatgtatca cgcccaggaa cgcattatga
    11101 atattcctgg ttcggaagta actggcatgc gcggcggcat tcataactcg gtcacccgcg
    11161 tttgtccgaa accaacgcat atgattggcg gttacgcgca gctggcctgg ggctttaact
    11221 actacggcac cgtcggctcg aaccgcgacg aattcatcat gatccgcaag atgaagaacg
    11281 ttaactggct ggatgatgaa ggtcgcgatc aggttcagga ggcgaaaaaa tgaaaatacg
    11341 ttcgcaagtc ggcatggtgc ttaacctcga taaatgtatc ggctgccata cctgttcggt
    11401 gacctgtaaa aacgtctgga ccggacgcga aggcatggag tacgcatggt ttaacaacgt
    11461 cgaaaccaaa ccgggcattg gttatccgaa aaactgggaa gatcaggaag agtggcaagg
    11521 cggctgggtg cgtgatgtga atggcaagat ccgcccgcgt ctgggcagca agatgggcgt
    11581 gattaccaaa atcttcgcca acccggtggt gccgcagatt gatgattact acgaaccttt
    11641 caccttcgac tacgaacatt tgcatagcgc gccagaaggt aaacatattc ctactgcgcg
    11701 cccgcgttca ctgattgacg gcaaacggat ggacaaagtg atctgggggc caaactggga
    11761 agaattgttg ggcggcgaat ttgaaaaacg cgcccgcgac cgcaacttcg aggccatgca
    11821 aaaggagatg tacgggcagt ttgaaaacac gttcatgatg tacctgccgc gcctgtgcga
    11881 acactgcctc aatcccagct gcgtggcgac ctgcccaagc ggtgctatct acaaacgtga
    11941 agaagacggc attgtgctga ttgatcagga taaatgccgt ggctggcgtt tgtgcattag
    12001 cggttgtccg tacaaaaaaa tctacttcaa ctggaaaagc ggcaagtcag aaaaatgcat
    12061 cttctgttac ccacgaattg agtcaggcca acccaccgtg tgctcagaaa cctgcgtggg
    12121 tcgcatccga tatctgggcg tgctgcttta cgacgccgac cgcatcgagg aagcggcgag
    12181 caccgagcgc gaagttgacc tctatgaacg ccagtgcgaa gtgttcctcg atccgcacga
    12241 tccctcggtg atcgaggaag ccctgaaaca aggtattcca caaaacgtga ttgacgctgc
    12301 ccagcgttcg ccagtctaca aaatggcaat ggactggaaa ctggcgctac cgctgcaccc
    12361 tgaataccgc accctgccga tggtctggta cgttccgccg ctgtcaccga ttcagtccta
    12421 cgctgatgcg ggaggtttgc cgaaaagcga aggcgtgctg cccgccatcg aaagcctgcg
    12481 tattccggtg caatatctcg ccaatatgtt gagtgccggt gataccggtc cggtactgcg
    12541 ggcgctgaaa cggatgatgg cgatgcgcca ctatatgcgt tcacaaaccg tggaaggcgt
    12601 tactgatact cgtgccatcg acgaagtagg tctgagcgtc gcccaggtcg aagagatgta
    12661 tcgttacctc gccattgcca actatgaaga ccgttttgtc atcccgacaa gccatcggga
    12721 aatggcgggc gatgccttcg cagaacgcaa cggctgcggt tttacctttg gcgacggttg
    12781 ccacggctcg gacagcaaat tcaacctgtt caacagtagc cgtatcgatg ccatcaacat
    12841 caccgaagtg cgcgacaaag cggagggcga ataatgcaga tcctcaaagt gatcggcctg
    12901 ttgatggagt atccggacga gctgttgtgg gaatgtaagg aggacgcgct ggcgttgatc
    12961 cgccgcgacg caccgatgct tacggatttt acccgcaacc tgcttaacgc gccgctgctg
    13021 gataaacagg ccgaatggtg cgaagtgttt gaccgtggac gtaccacatc gctgctgctg
    13081 ttcgaacatg ttcatgccga gtcccgcgat cgcggccagg caatggtgga cctgctggcg
    13141 gagtatgaaa aggtcggcct gcagttggat tgtcgagaac tgcccgatta tctaccgctg
    13201 tatctggagt acttaagcgt actgccggac gatcaggcga aagaaggctt gctcaacgtt
    13261 gcgccgatcc tcgccctgct tggcggacgc ttaaaacaac gcgaggcacc gtggtacgcg
    13321 ttatttgaca ccctgttgca actggcagga agtacacttt caagtgacag tgtcacaaaa
    13381 caggtgaaca gtgaggagcg cgatgacacc cgccaggcac tggatgcggt gtgggaagag
    13441 gaacaggtga agtttattga agataacgcc acggcgtgtg acagctcgcc gcttaatcaa
    13501 tatcagcgac gctttagcca ggatgtcgcg ccgcaatatg tcgacatcag tgcgggaggt
    13561 gggaaatgat tcagtatctg aacgtctttt tttacgatat ctacccgtac atttgtgcga
    13621 cggtgttttt ccttggcagc tggctgcgct atgactacgg gcagtacacc tggcgcgcct
    13681 cctcaagtca aatgctcgat aaacgcggga tggtgatatg gtcgaatctg ttccatattg
    13741 gcattttggg gattttcttc gggcacctgt tcggcatgtt aacaccgcac tggatgtacg
    13801 cgtggtttct gccggtggcg gtaaaacagc agatggcgat gatcctcggc ggaatatgcg
    13861 gggtattgac gctgattggc ggtgcggggc tactggtgcg tcgtttaaca aatccgcgta
    13921 tcagggcgac gtcttctggt gcggatatcc tgatcctcgc cattttgtta attcagtgca
    13981 ttcttgggtt aaccaccatt cctttttcag cacaacatcc ggacggcagt gaaatgatga
    14041 agctggtggg ctgggcgcaa agcattgtca ctttccaggg cggtgcatct acgcatcttg
    14101 atggtgtcgc ctttatattc cgcgttcatc tggtgcttgg aatgaccatc tttctgctct
    14161 tcccgttcac ccgactggtg catgtctgga gcgcgccgtt tgagtacttt actcgtcggt
    14221 atcagattgt gcgttcgcgg cgttaattct ctcgcgggcc ggatagctct tcgctctccg
    14281 gccacttgca ggctatgata gttctccagc cacaggagaa cattaatgaa accgcaggtt
    14341 taccacgtcg atgcttttac ctctcaaccg tttcgcggca attctgccgg tgttgttttc
    14401 cccgccgata atcttagcga agcgcaaatg cagcttatcg cccgcgagtt aggtcattcg
    14461 gaaactgctt ttctgctgca cagcgacgac agcgatgtgc gcatccgcta ctttacgcca
    14521 acggttgaag ttccgatttg tggtcacgcg acagtagctg cgcactatgt acgtgcaaag
    14581 gtgttaggtt taggaaattg caccgtctgg caaacgtcgc tggcaggaaa acatcgtgtg
    14641 actatcgaaa agcaaaatga tgattatcgt atttcgctgg agcaaggtac gccgggcttt
    14701 gaaccaccgc tggaaggtga aacacgtgca gcgattatca acgcgcttca tctcactgaa
    14761 gacgatattt tgcaaggctt gccaatccag gtggctacca caggacactc aaaagtgatg
    14821 attccactga agccggaagt ggatatcgac gccctttcgc ctgaccttgc tgcgctgacc
    14881 acaatcagca aacagattgg ctgcaatggt ttcttcccgt tccagatccg cccaggcaaa
    14941 aacgaaaccg atggtcgcat gttctcgcct gcgattggta ttgtggaaga tccggtgact
    15001 ggcaatgcca atggcccgat gggtgcatgg ttagtacatc acaacgtatt gccacacgat
    15061 ggcaacgtgt tgcgtgttaa aggccatcag gggcgcgcat tagggcgtga cggcgtgatt
    15121 gaagtgacgg tgacaattcg tgataaccaa ccggagaaag tcaccatttc tggcacggcg
    15181 gtgattttgt ttcatgccga atgggcaatt aaactctgaa accgttgcct gatgcgcttc
    15241 gcttatcagg cctacaaccg gactaatgta tgccggatta ggcatttacg ccacatccgg
    15301 caacctgaca taaattattt tcccgcctcc gggtgcgtat caaacgccgc catcaccagc
    15361 gctaactcat ccacagtaaa gccatgtttc gcatcatcta cgcccagacc aaactgttcc
    15421 tgcatcacgg catataacga cgccacatct ggtagatttc gctgctcaac tgcgtgccca
    15481 ttttcataat gggtaaaatg aaaattggtc agtgtcagct tgccgccgtc cggtaaatgg
    15541 cggcacatca gcaaatgatg gcgaaaatgc gactgcggcc aatgcgccga ccagaaattg
    15601 cccatcacat aatcgctttg ttgctgctcg cagagatcaa aacggtacat cgactgccag
    15661 tgctgatgat gattaaactg caacacccaa tcatcaccct cctgcaacaa ccgatactct
    15721 ccgtgtggcg tggtctgcac gagctcggga actaaccgaa tcggcgcggt tagcgtctgt
    15781 ccaccgaaac cgacatcagc aatccatttt tccccttcca gttccaccaa caacaaacga
    15841 tgggtgcgcg gcggtaatgc tggcggattt gataacacta cgcgccctaa caagctgcga
    15901 acgttaaacc ccagctcgcg taacacacgc tcaaacacgc cattctgctc aaaacagtaa
    15961 ccgccacgac gggctgtcac cagtttctct tccagcgatt gatcatcaag ctgtatttcc
    16021 cttggcagca aaacgtcgag gttttcaaat ggaatggtgc aattgtgttt caggtgcaat
    16081 gcacgcagcg tatcaatatt ggccgcagca gctcccgacc agttaatacg ggcaaaataa
    16141 tgattcagaa tgggcgtcat caggcgtttc ccttagtggt tttctcaact tataacctgg
    16201 aatagacgct gcaccggcta ttttgtgcga tcgcggacat gcatcacgtt cgcaaatcgc
    16261 caatcccgtt aatttcgtta cttatagcgt tacgagctac gcttttaaaa cacccttctt
    16321 agcgaggcga taccgtgagc cgattcatcc ccgtcgaatt acaccatgcc agccgtctgt
    16381 taaatcatgg cccaactgtc atgatcacca gtttcgatga gcaatcccag cggcgtaaca
    16441 ttatggctgc agcctggtca atgccggtgg agtttgaacc gccacgcgtc gcgattgtgg
    16501 tagataaatc gacatggacc agggagttga ttgaacgtaa cggtaaattt ggcatcgtta
    16561 tcccgggcgt tgcagcaact aactggacgt gggcggtggg aagtgtgtcg gggcgtgatg
    16621 aagataagtt taattgctat ggtattccgg ttgtgaaagg cc
//
LOCUS       DAMZFT010000110        16632 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.110, whole genome shotgun sequence.
ACCESSION   DAMZFT010000110 DAMZFT010000000
VERSION     DAMZFT010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16632)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16632)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16632
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.110"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            214..453
                     /locus_tag="PN977_003683"
     CDS             214..453
                     /locus_tag="PN977_003683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000389730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence RhuM family protein"
                     /protein_id="HDI6043682.1"
                     /translation="MCLFIRSPTPRNTVNPDIFYWLQEYLIKSFVMDDERLKNPPVGS
                     SVLERIRDIRASMRRVYLQVICPPADSQPTVSPLR"
     gene            835..2019
                     /locus_tag="PN977_003684"
     CDS             835..2019
                     /locus_tag="PN977_003684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter"
                     /protein_id="HDI6043683.1"
                     /translation="MQKTATTPSKILDLTAAAFLLVAFLTGIAGALQTPTLSIFLADE
                     LKARPIMVGFFFTGSAIMGILVSQFLARHSDKQGDRKLLILLCCLFGVLACTLFAWNR
                     NYFILLSTGVLLSSFASTANPQMFALAREHADRTGRETVMFSTFLRAQISLAWVIGPP
                     LAYELAMGFSFKVMYLTAAIAFVVCGLIVWLFLPSIQRNIPVVTQPVEILPSIHRKRD
                     TRLLFVVCSMMWAANNLYMINMPLFIIDELHLTDKLAGEMIGIAAGLEIPMMLIAGYY
                     MKRIGKRLLMLIAIVSGMCFYASVLMATTPAIELELQILNAIFLGILCGIGMLYFQDL
                     MPEKIGSATTLYANTSRVGWIIAGSVDGIMVEIWSYHALFWLAIGMLGIAMICLLFIK
                     DI"
     gene            2130..3053
                     /locus_tag="PN977_003685"
     CDS             2130..3053
                     /locus_tag="PN977_003685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HDI6043684.1"
                     /translation="MGSTRKGMLNVLIAAVLWGSSGVCAQYIMEQSQMSSQFLTMTRL
                     IFAGLILLTLSFVHGDKIFSIINNHKDAISLLIFSVVGALTVQLTFLLTIEKSNAATA
                     TVLQFLSPTIIVAWFSLVRKSRPGILVFCAILTSLIGTFLLVTHGNPTSLSISPAALF
                     WGIASAFAAAFYTTYPSTLIARYGTLPVVGWSMLIGGLILLPFYARQGTNFVVNGSLI
                     LAFFYLVVIGTSLTFSLYLKGAQLIGGPKASILSCAEPLSSALLSLLLLGITFTLPDW
                     LGTLLILSSVILISMDSRRRARKINRPARHE"
     gene            complement(3057..3875)
                     /gene="nlpA"
                     /locus_tag="PN977_003686"
     CDS             complement(3057..3875)
                     /gene="nlpA"
                     /locus_tag="PN977_003686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpA"
                     /protein_id="HDI6043685.1"
                     /translation="MKLTTHHLRAGAALLLAGILLAGCDQSSSDAKHIKVGVINGAEQ
                     DVAEVAKKVAKEKYGLDVELVGFSGSLLPNDATNHGELDANVFQHRPFLEQDNQAHGY
                     KLVAVGNTFVFPMAGYSKKIKTVAQIKEGATVAIPNDPTNLGRALLLLQKEKLITLKE
                     GKGLLPTALDITDNPRHLQIMELEGAQLPRVLDDPKVDVAIISTTYIQQTGLSPVHDS
                     VFIEDKNSPYVNILVAREDNKNAENVKEFLQSYQSPEVAKAAETIFNGGAVPGW"
     gene            4084..4377
                     /locus_tag="PN977_003687"
     CDS             4084..4377
                     /locus_tag="PN977_003687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6043686.1"
                     /translation="MKPTMLLMITVFLTFPAISQAESPFSSLQSAKEKTTVLQDLRKI
                     CTPQASLSDEAWEKLMLSDESNKQHIREAIVAMERNNQNNYWEALGKVECPDM"
     gene            4679..5176
                     /locus_tag="PN977_003688"
     CDS             4679..5176
                     /locus_tag="PN977_003688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001604188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SycD/LcrH family type III secretion system
                     chaperone"
                     /protein_id="HDI6043687.1"
                     /translation="MESANLDLEENKEIASKFERALGMGATLAELHGITPDTLEGVYA
                     YAYNFYEKGRLDEAELFFKFLCIYDFQNYNYLKGYAAVCQLKKDYQKAFDMYHICLML
                     SPDNDFSLVYYMGQCQMGLKNIKMATELFNTVVTYSQNEKIKEMATTYLELLTANSEE
                     EVQTE"
     gene            5186..5368
                     /locus_tag="PN977_003689"
     CDS             5186..5368
                     /locus_tag="PN977_003689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6043688.1"
                     /translation="MNINGKNASFTSEELARKYHEEGTVDLKNNHGDKINEKNNNKHI
                     TYFPVDAILDGNKHLP"
     gene            5424..7205
                     /locus_tag="PN977_003690"
     CDS             5424..7205
                     /locus_tag="PN977_003690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001600713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YopB/SseC family type III secretion system
                     translocon subunit"
                     /protein_id="HDI6043689.1"
                     /translation="MSAPITGQTITFEQISETLRTQYSDAEKRLQDSSKTQVDPMRLN
                     KNPKSLDNDIRARLENKPMLAPPTIQVSDSDNATTAKTNDARLTMILGNLTGIADQDI
                     TTRLHNNLDSTLLRHEMAHNKFRELSDAYSSSLDDAQKADDIMHQANNNYNAVDKKVQ
                     SLEKKVNTLNQELSQLQPGDPQYNKVLTQKNAAEKTLTLSLQKKSLAEKSLNTAIMDA
                     DAAIGQSMEIFDEIQQQEQINNFTTNICLTQENQKNRNATATFILLITSVMEVIGDTN
                     CDSIKNQSEVMKEINHVRENKLNETARKYTTTTKVLKIVNECVTVVTFAVSAVLIVVG
                     LLAAVPSGGSSIAGALALIGGIAGAVVLGVDITCQIALGTTATGWILGKVVEGLSAAI
                     KTVDPTLLAITALLDVIGVDQDTIELVKSIYASAAASIVMATVMIGAAVICSVAIGAV
                     VSALSKTAAEEVTKEITSTIKSTIESIINSVSKNIIKVLDSVCSVLQTSAVVLKLIAK
                     ISNGLEKIGLLICAIATSTMNCFVAGNSADMAILQQDMSNLSKTREQMLSVLQRVDKT
                     VEQEVSQMVRVLQHRTEALKFASHSIV"
     gene            7218..8369
                     /locus_tag="PN977_003691"
     CDS             7218..8369
                     /locus_tag="PN977_003691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000257045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6043690.1"
                     /translation="MATYEIKNATSIHNINNLDKTIFDRDYGKENNIPNDNKIVQYYN
                     LKDIKHDISSQFIILKNTDREVCPELRVKLLDNLNYSRNTLDNLRGDLDQATISFSQS
                     SSSAEQSSIKISKYNILDMLDNLTMRQQAVMEEFLRVFTSMYKASAMSEAIMSNVFEQ
                     LVQYQKTETVHATMASSAGVLAGSTVGAAGAAASVVANFRSHPEKMQTLTPDQKHTIS
                     GTDDKTQRGLKREVQDQQAYLKQIDEEIKTTTKDTTLDEEQKEETLKYYDKEKETTEE
                     KIRDKNERISIKHNRKYNKYFTIGKADVLSQLVNQSTQASLRILESTTHAEKDIVEQA
                     KNVMHSVMSSYLDQINRDVQGRDQALNLLQQIIQTIIDTDSAIVQGIRS"
     gene            8413..9762
                     /locus_tag="PN977_003692"
     CDS             8413..9762
                     /locus_tag="PN977_003692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000608445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IpaD/SipD/SspD family type III secretion system
                     needle tip protein"
                     /protein_id="HDI6043691.1"
                     /translation="MINKDNNLNIDILRQQNANIPLQEASSQSEEPHQLENSKSEGNL
                     HNSASKDNNIGAMLKKDVEKLSTLVSERSPRNEDWKCQHDIQLNFVKNMLRNFPTENL
                     SEKGSALLHESYSLIKNVLQLTENVNTIDGKLKDPAKILTLHNSVRKTLANVAHNTTG
                     SSAMASTKGTLNHAIASLESSAPEMRSSATSSTLTASSSNEFKSDKYICSDIADLMSV
                     LGSDYLEIYANSVEIMSAYWQDFSEHIQSNMGKWTHSNKKGDAIVFDVNAFQKALMHF
                     YYIDKYPNGDFHYHYNPDYVLYPPAPADKIGVPLEEAEKWYAALGLPVIPPDPKHRTP
                     SPIVEVEPQGSGLYVIIPNPQIIDSMSQSSDSMVHRDDKGKEKNISKEFTGYEISTAE
                     YQAWLAGYNGQAENMKTDVQVITTKYSTANSTYDTIIKLLSSTITALFDSAKDYLRF"
     gene            9965..11662
                     /gene="eilA"
                     /locus_tag="PN977_003693"
     CDS             9965..11662
                     /gene="eilA"
                     /locus_tag="PN977_003693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001176839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HilA family transcriptional regulator EilA"
                     /protein_id="HDI6043692.1"
                     /translation="MQLQHGKNNTQQYIFGDFVLKNNGILLFKNKEYHIPPKELGVII
                     LLLNADGEIVSKEEIIDKVWSASVASDESLTRCIYALRKLLHENKQCKYIETVYGRGY
                     RFTVPIVIVTDNEPVKSTTTLAVFPFRTEGSINVLKLHYELVQGLSKYAFCGLDILPA
                     SVTNEASDFSTIHQFINKTGPEYYIMGQVVHYGKNWRLFIELIHARTHKLIDHQSIDF
                     NPDNPLSVLLSQVINILIEKIPNINFKSINTQQMPSLDSAVMYMNGRMEMYRYTPESL
                     KRALVIFRDCVSTQPQNTLPYCCLAECHISLALLGISEQKQAITTAVTSIEKALEINP
                     SNSQALGLLGLISGLKDEHSVSNVLFKQAHLLKPNSPDVYYYQSLLYFLNGDLARAFN
                     LIEKSIALEPNKMGISILKLIILYYTSPLDNAISFALNLNSQNTCNNPIITSIQAMFM
                     ALKGHNDKAKSLLLKLEPEHGLDYTCVNSLYTKFLIYGASIKNDIMKLLANINTNKIN
                     GVILPLIYTVYGKKEYEKRWQQLIKENDLWSNVLLHDPRLLSVKNEFNTIGVMRTSA"
     gene            complement(11714..>16632)
                     /locus_tag="PN977_003694"
     CDS             complement(11714..>16632)
                     /locus_tag="PN977_003694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HDI6043693.1"
                     /translation="ADNGATVAPVVTTQPDGTVEISVTSQTAGSSAVTVSINSSSQSR
                     DVTFIADVRTAQIADLVVIKDDSVADGAMANMLRARVSDVFGNALAGQTVSVMADNGA
                     AVASTMTTKPDGTVEISVTSQTAGISVVTASINNSSQSQNVTFVADVRTAKIADLVVS
                     QDNAVADGSTANTLRARVTDAFGNTLAGQTVSVMAGNGATVAPTVITEPDGTAEISVT
                     SQTAGVSAVTASINNSSQSRDVTFIADIRTAQIASLEVTQDNAVADGAMANTLQVRVT
                     DANGNTLAGQAVSVMAGNGATVAPAVTTQPDGTVEIPVTSQTAGASAVTASINNSSLS
                     RDVTFIADVRTAQIAELVVIKDGSAADGATANTLQARVTDAFGNALAGQTVSVLADNS
                     ATVAPAVITEPDGTVDISVTSQTAGISTVTATINNHSLSQSVMFIADVRTAQIADLVV
                     IKDGSEADGATANTLRARVTDAFGNALAGQTVSVLADNGATVAPTVITGQDGTVEISV
                     TSQTAGISTVTATINSSSQSQNVTFIADVRTAQIAELVVIKDGSAADGVMANMLRARV
                     TDAFGNALAGQTVSVLAGNGATTAPTVTTQPDGTVEISVTSQTAGISAVTASINNSSQ
                     SRNVTFIADVRTAQIADLVVIKDGSEADGATANTLRARVTDAFGNALAGQTVSVTAGN
                     GATVAPTVTTQPDGTAEISVTSQTAGVSAVTASINNSSQSRDVTFIADISTAQIADLV
                     VIKDGSEADGATANTLRARVTDAFGNALAGQTVSVMAGNGATVAPAVTTQPDGTVEIS
                     VTSQTAGISAVTASINSSSQSRDVTFIADVRTAKIAELEVIRDNAVADGSTANTLQVK
                     VTDANGNTLAGQTVSVLAGNSATVASTVTTKPDGTVEISVTSQTAGTSTVSASINNSS
                     QSQNVTFVPGDASQLTSTVETNKSNYTVGETITITVTLRDAFDNLVTGAASQLAADGV
                     LTVAGTDPSETGSWVESGGVYTTTRMATIASTNQHANLQLQTWSDGVTSDRYDIQSGS
                     PAQATSTIATDKNAYTAGDTITVAVTLKDAHGNLVEGGESLLSGDNVTVEGAVRSGGW
                     SETAGVYTATWSAQMAGDSHHATLKLSEWGSSKQSESYSIHSGAPVQANSAIRTDKSA
                     YIAGEPLTVTITLRDEFGNPALGLTSEVIESYIDSFAVGGATPDSMRWVEQNNGEYTI
                     VWTAWIAEENLVASLKLKTWAEEIKSSLYGIQPGAAAKTQSTIVADKTIYIAGDSITV
                     TVVLKDAQGNFITDGVVQLNEENVQVRNADPIQGNNWVYNGNGQYQRQYMAHFAEANL
                     NAQLKMAGWSDANYSNNYTIKPGEVSPLGSQLRIREVLVVEGADLPVSVLLVDDFGNP
                     VDNGLDLLDDTVYLQNVEKKEGEKWRYVGDGIYERTYMAYQEGENLTSFMEIKGWRIY
                     GQPSYTILPFVEVELLSVNGVKFRATDGFPETGFDGAKFTLLLTHNMKNTDYNWTAGI
                     YGINVDSNGEVTLSVLIRSEVTITGKPKNGKGNDVVFKFKIKKWFTSLGATSSNTWDI
                     INTSCSYGQMPSSLELAQRPSGGVVPRKVGTLWGEYGNLKTYGNAFSGTDYWTSTQLM
                     GVHEKFNPETGISELGTGKSSGLCVEYY"
BASE COUNT         4636 a         3921 c         3486 g         4589 t
ORIGIN      
        1 tctgaaataa aaggagaaaa cagaaaggtg aacagtgtga acagttcttt cgaaaaaaaa
       61 tttttttcct gagttaacat gcgtgaagat acgtgtaaaa attaatcgat agatattata
      121 tgtataagga tgtgtataaa tgaattataa tagaaaaata taaacgattt gaatcaaatg
      181 gttatatggc aattttgact cctgtgatcg ccaatttgcc tcttcatacg ttctccaacc
      241 cctcgtaata ctgtcaatcc agatattttc tactggcttc aggaatacct gatcaaaagt
      301 tttgtgatgg acgatgagcg actgaagaat ccgcctgtcg gttcttctgt cctcgaacgc
      361 atccgcgata ttcgcgccag catgcgccgt gtttatttgc aggtgatttg cccccctgct
      421 gactctcagc caacggtatc gccattaagg tgaagatgtt gttttacacg cttctgcaca
      481 ccctttttta tacgactatt ctatttcttt aaccgctgac gaaaaaactg acctataaac
      541 ttcaccaata atcagactgc catcaagatt aatttattca tgtaatatat caaatcaact
      601 tgttttattt ttactatcaa aaatagctca ttgccttact ataattaata tataaattcg
      661 gtgattaact cttaacatgc ttttgctaat aatctcaatt gctggcccta taatattgcg
      721 ctagcattgc tttctggttg tatcagcgat actcaaaaat tcttaataca atactcattc
      781 gactggtact tatttgtaac tcagttatat tttgtcgccc ggtgattcag aagaatgcaa
      841 aaaacggcta ctactccatc aaaaatactt gatctcactg ccgcggcatt tttacttgtc
      901 gcctttctga cgggtattgc gggcgctctc cagactccca ccctaagtat attcctcgca
      961 gatgaactga aagcccgtcc tataatggta ggttttttct tcaccggtag cgctattatg
     1021 gggattctgg tcagtcaatt tctggcaagg cactccgata aacaaggcga ccgtaaatta
     1081 ctgatcctgc tatgttgctt atttggagtg ttggcctgca cgctttttgc gtggaatcgc
     1141 aactacttca ttctcctctc tacgggcgta cttctgagta gttttgcttc cactgcaaac
     1201 ccgcaaatgt tcgccctcgc ccgtgaacac gccgacagaa caggccgtga gacggtcatg
     1261 ttcagtacat ttttacgtgc tcagatctcg cttgcctggg ttatcgggcc accgctcgct
     1321 tatgaactgg caatgggatt tagttttaaa gtgatgtatc tcaccgctgc catcgcattt
     1381 gttgtttgcg gactgatagt ctggttgttt ttgccatcaa tacaaagaaa tattcctgtc
     1441 gttacccaac ccgtagaaat tttaccctcc atccatagaa agcgggatac acggctactt
     1501 tttgtagtct gttcaatgat gtgggcggcg aataatctct acatgataaa tatgccgcta
     1561 tttattattg atgaactgca tctaaccgat aaactggctg gagaaatgat tggtatcgct
     1621 gccggtctgg aaattccgat gatgttaatc gcaggctatt acatgaaacg tattggcaag
     1681 cggctattaa tgctcattgc tatcgtgagt ggtatgtgtt tttacgccag cgtactcatg
     1741 gcgacgactc cggcaattga gctggaattg caaattctaa atgccatttt ccttggtatt
     1801 ctctgtggta tcggcatgct ttattttcag gatctgatgc ctgaaaaaat aggctctgcg
     1861 acaacgttat atgcaaatac ctcacgcgtc ggctggatta tcgccggctc tgttgacgga
     1921 attatggttg aaatctggag ctaccacgcg ttgttctggc tggcgatagg gatgttgggt
     1981 attgcgatga tttgcctgct gtttattaaa gatatttagc tcaccagtct gggctaagaa
     2041 aaatcggcgt ggcgcaaaca gagccatcct cgccattttt gctatcatgc ctgcatacat
     2101 aaacgacaaa acagtatgca gagggaaaaa tgggttccac cagaaagggg atgctgaacg
     2161 ttctgattgc cgccgtgttg tggggaagtt caggggtctg cgcgcaatac atcatggagc
     2221 aaagccagat gtcgtcgcag tttttgacta tgacgcgttt gatattcgcc ggtttgattc
     2281 tgctgacgct gtcatttgtt catggcgata aaatcttttc tattattaac aatcacaaag
     2341 atgccattag tctgctgatt ttttccgtgg ttggcgcgct aaccgtacag ctcacttttt
     2401 tgctaaccat cgaaaaatcg aacgcagcca cggcaacggt gctgcaattc ctctcaccga
     2461 cgattatcgt cgcctggttc tcactggtac gtaaatcgcg cccgggcatt ctggtttttt
     2521 gcgctatttt gacatcgctg atcgggactt ttttattggt gacacacggt aatccgacgt
     2581 cattatcgat ctctcctgcc gcgttattct ggggcattgc ctcggcattt gctgctgcat
     2641 tctataccac ctatccctca acgctgattg cccgctatgg cacgttaccg gtcgtcggct
     2701 ggagtatgct cattggtggt ctgatcctgc tgccatttta tgccagacaa gggacaaatt
     2761 ttgtggttaa tggcagtttg attctggcgt ttttttattt ggtggtcatt ggtacgtccc
     2821 tgacatttag tctgtacctg aaaggagcac aattaattgg cggtccaaaa gccagcattt
     2881 tgagctgtgc agaaccatta agtagcgcgc tgctctcttt gctgttgctg gggatcacat
     2941 tcacattacc ggactggctg ggaacgcttc tgattctgtc atcagtgatt ttgatttcaa
     3001 tggattcccg tcgccgcgcc agaaaaataa atcgtccggc gcggcatgag tgagaattac
     3061 cagccaggca ccgcgccacc gttaaagatg gtttctgccg ctttagcgac ttcgggcgat
     3121 tgataagatt gcagaaattc cttcacgttt tctgcattct tattatcttc ccgtgccacc
     3181 agaatattca catacggcga attcttatct tcaataaata cgctgtcgtg caccggagaa
     3241 agcccggtct gctgaatgta agtggtgctg ataatcgcca catcaacttt gggatcatcc
     3301 agtacgcgcg ggagctgcgc cccctcgagt tccataatct gcaaatgacg aggattatcg
     3361 gttatatcca gtgcggtagg taataaccct tttccctctt tcagagtaat tagcttctct
     3421 ttttgcaaca gtaaaagcgc gcgaccaagg ttggtcgggt cgttggggat cgccactgtc
     3481 gcgccttctt ttatttgcgc cacggttttg atttttttgg aataacccgc catagggaag
     3541 acaaacgtat ttcccaccgc taccagttta taaccatgcg cctgattatc ttgttcaaga
     3601 aagggccgat gctggaagac gttcgcatca agctcgccat gatttgtcgc atcgttcggt
     3661 agcaacgaac cgctaaatcc taccagttca acatcgaggc catatttctc ttttgccacc
     3721 tttttagcga cttccgcgac atcttgttct gcgccattta taacgccaac tttaatgtgt
     3781 tttgcatcgc tgctactctg gtcgcaacct gccagcagaa ttcctgccag caataatgcg
     3841 gcccccgccc gtagatgatg tgttgtcagt ttcatttttt atcctttttt taatcattaa
     3901 ctgactataa tggcaagatc actacgattt aaaaaacgaa aagctatcga atagaagaaa
     3961 aaggtataaa aaactggcac ttcttactga ccacgcaaac acctttcgta cagcatgatg
     4021 agagcggtaa aagcaaacta cacttatgct tcattcgatg cacatttgca gaaggttgcc
     4081 gctatgaagc caacgatgct actcatgatt accgtattcc tgacttttcc agccatttct
     4141 caggccgagt cgccattcag ctcgctacag tcggcaaaag agaaaaccac ggtattgcaa
     4201 gatttacgta aaatttgcac gccacaggcg tcattatcag acgaagcgtg ggaaaaatta
     4261 atgttgtctg atgagagtaa caaacaacat atccgcgaag ctattgtggc gatggagcgt
     4321 aataatcaga ataactactg ggaagcgctg ggtaaggtgg aatgcccgga tatgtgaatt
     4381 tcgcacacct ccgcgctcag gccagcagcc gctgacttaa gctcgcacat cagtaaatca
     4441 cctgcttttt caaattccaa tcaaaattga acccgttcat caggattgtg gataaatttc
     4501 cctctacatt attttcattt catcacatag aaaatcttat ctcattgaaa caatacttgt
     4561 ttttaatgca gaaaacgaag cccaacggat gggcataaaa atgagcgaat tttgatatca
     4621 ccaccctcta aaaaatggtg aagtctgaac aaagaatgtt cgaaaggaga gagtagagat
     4681 ggaaagcgca aatctggatt tagaagaaaa taaagaaatt gccagtaagt tcgaacgtgc
     4741 attaggtatg ggagcaacgc ttgcagagct gcatggcata accccggata cccttgaagg
     4801 cgtttatgct tacgcttata acttttatga aaaaggaaga cttgacgaag ctgaactttt
     4861 ttttaaattt ctctgtattt atgatttcca aaattataac tacttaaaag gatatgccgc
     4921 cgtctgtcaa ttgaagaaag attatcaaaa ggcattcgac atgtatcata tctgtttaat
     4981 gctatctccc gataacgatt tctctttggt ttattatatg ggtcaatgcc agatgggctt
     5041 aaaaaacatt aaaatggcca cggagttatt taatacagtc gtaacctata gtcaaaatga
     5101 aaaaattaaa gagatggcta caacttatct tgaacttttg acagccaatt ccgaagagga
     5161 agttcaaaca gagtgagtcg gcaaaatgaa cataaacgga aaaaacgctt cttttacgtc
     5221 tgaagagtta gccaggaaat atcatgagga aggcacagtt gatttaaaaa ataaccatgg
     5281 cgataaaata aatgagaaga ataacaataa acacattact tactttcccg ttgatgccat
     5341 tttagatggg aataaacatc tgccatgagt taaataaaaa agaattaatt acacccgatg
     5401 atagcttaaa aaggaggtga attatgtcgg caccgattac gggacaaacg atcacgtttg
     5461 aacaaatcag tgaaacattg cgtacacagt atagcgatgc ggaaaaaaga ctgcaggaca
     5521 gcagcaaaac gcaagttgat cccatgcggc ttaataaaaa cccgaaatcc cttgataacg
     5581 atattcgcgc tcgtcttgaa aacaaaccga tgcttgctcc gcctacaata caggtttcag
     5641 attcagacaa cgccactaca gcaaagacga atgatgcccg cctgacaatg attttgggta
     5701 atttaacagg cattgccgat caggatatta caacacgttt gcataataac cttgacagca
     5761 ccctgcttcg acacgaaatg gcacataata agtttcgtga attaagtgac gcttactcct
     5821 cgtcattaga tgatgcgcaa aaagccgacg atatcatgca tcaggccaat aataattaca
     5881 atgcggtcga taaaaaggtg caatcgctgg agaaaaaagt caacacattg aatcaagagc
     5941 tgtcgcaact gcaacctggc gatccgcaat ataataaagt actgacgcaa aaaaatgcgg
     6001 ctgaaaaaac actcacgctg tcattacaaa aaaaatcgtt agctgagaaa tcgttaaata
     6061 cagccattat ggatgctgat gctgcgatcg gccaaagtat ggaaattttt gacgaaattc
     6121 aacagcaaga acagattaat aacttcacca ccaatatttg cctgacacag gaaaaccaga
     6181 aaaatagaaa cgcgacagcc acatttatcc ttttgattac ttcagtaatg gaagttattg
     6241 gtgataccaa ttgcgactct attaaaaatc aatctgaggt aatgaaagag attaaccatg
     6301 tcagggaaaa taaactcaat gaaacagcac gtaaatatac taccacgact aaagttttaa
     6361 aaatcgttaa tgagtgtgta actgtagtta cctttgccgt aagcgcagtc ttaattgttg
     6421 ttggtcttct tgctgcagtt ccaagtggtg gttcaagtat tgcgggtgca ttggcactta
     6481 ttggaggaat agcgggcgca gtagtattag gtgtagatat cacttgtcaa atcgctctgg
     6541 gcaccacggc taccggctgg attttaggga aagttgtaga aggtctttcg gctgcgatta
     6601 aaacagttga tcccactctg ctcgcaatca ccgcgcttct ggacgttatt ggtgtcgatc
     6661 aagatacgat tgaactggtt aaaagtattt acgcaagtgc agccgcttct attgtcatgg
     6721 cgacagtaat gattggtgca gctgtaatat gttctgtagc aataggggcc gttgtttctg
     6781 cattatcaaa aacagccgcc gaagaagtca ccaaagaaat aacaagcact ataaaatcaa
     6841 ccattgaatc cattattaat tccgtttcaa aaaatattat aaaggtatta gacagcgtat
     6901 gcagcgtgct acaaacatca gccgtagtgt tgaagttgat tgccaaaata agtaatggcc
     6961 tggagaaaat aggcttactc atctgtgcaa tagcaacatc gacgatgaat tgttttgttg
     7021 ctggaaactc tgccgacatg gcaattttac aacaggacat gagtaatcta tcaaaaacgc
     7081 gtgaacaaat gctttcagta ttgcaaaggg tggataaaac cgtcgaacaa gaggtaagcc
     7141 agatggtaag agtattacaa caccgaactg aagccttaaa atttgcttcc cattctatcg
     7201 tataagaggg ttttaacatg gccacctatg aaattaaaaa tgcgaccagc atacataata
     7261 ttaacaatct cgataaaaca atctttgata gagattatgg taaagaaaac aacataccaa
     7321 atgataataa aattgtgcaa tactataacc ttaaagatat taagcatgac ataagctctc
     7381 agtttataat attaaaaaat acagacagag aagtctgtcc ggaactaaga gtaaaacttt
     7441 tggataatct taattactca aggaatacgc tagataattt gaggggtgac cttgaccaag
     7501 ctacaatttc attcagtcaa tcaagcagtt ctgcagaaca gagttctatt aaaatatcta
     7561 aatataatat ccttgatatg ctagataatt tgaccatgcg acaacaggct gttatggagg
     7621 aatttttaag ggtattcaca tcaatgtata aagcctctgc tatgtctgag gctattatga
     7681 gcaacgtttt tgaacaactt gtccagtatc agaaaacaga aaccgtacac gccacaatgg
     7741 caagttcggc aggtgttctt gccgggtcaa cagttggcgc agctggagct gcagcatctg
     7801 ttgtagcaaa tttcagaagc catcccgaaa agatgcaaac ccttacacct gatcagaagc
     7861 acactatcag tggaactgat gataaaacac agcgaggatt aaaaagggaa gttcaagatc
     7921 aacaagctta tctcaagcaa atagatgaag aaataaaaac aacaacgaaa gatacaacac
     7981 ttgatgaaga acaaaaagaa gagaccctga aatattacga taaagaaaaa gaaactacgg
     8041 aagaaaaaat tagagataaa aacgagcgta ttagtataaa acataacagg aaatataata
     8101 aatattttac tataggtaaa gcagatgtac tttcacagct tgttaatcaa tcaactcaag
     8161 catcacttag aattctagaa agtactactc atgcagaaaa agacattgtt gaacaggcta
     8221 aaaatgtcat gcattcagtt atgagtagct atctggatca aattaatcgt gatgttcaag
     8281 gaagagatca ggcactcaat ttattacagc aaataataca aaccattatt gatactgata
     8341 gtgctattgt tcaaggaatt cgctcataaa ttattgcaat aatagtttct tatatttaag
     8401 gaggaatgac acatgattaa taaagataat aatttaaata tagatattct acgtcagcag
     8461 aatgcaaaca ttcctcttca agaggcatcc tcgcaaagtg aggagcctca tcaactggaa
     8521 aacagcaaaa gcgagggtaa tttgcataac agcgccagta aggataataa tattggcgct
     8581 atgctcaaga aagatgttga aaaactatca actttagtca gtgaacgtag cccgcgaaat
     8641 gaagactgga aatgccagca tgacatacag cttaacttcg ttaaaaacat gctcagaaat
     8701 ttcccaactg agaacctgtc agagaaagga tctgcgctct tacacgaaag ttattcgctg
     8761 ataaaaaacg ttctgcagct tacagaaaat gttaatacta ttgacggaaa attaaaggat
     8821 cccgctaaaa tattgacctt gcataactct gtgagaaaaa ccctggctaa cgttgctcac
     8881 aacacaaccg ggtcaagtgc aatggcatcg accaaaggta ccttaaatca cgctatcgct
     8941 tctttagaga gttctgcgcc tgaaatgaga agttcagcaa ccagcagtac tttaacggcc
     9001 agtagttcaa acgaatttaa aagtgataaa tatatatgca gtgatattgc tgatttgatg
     9061 agtgtgctcg gtagcgacta cctcgaaatc tatgcaaata gcgttgagat aatgagcgca
     9121 tactggcagg acttcagtga acatatccaa agcaatatgg gaaaatggac tcactctaat
     9181 aaaaaaggcg atgcgatagt ttttgacgtc aatgcattcc aaaaagcctt gatgcatttt
     9241 tattatatcg ataaatatcc taatggtgat ttccactatc attacaatcc agactatgtt
     9301 ttataccctc cggcacctgc tgacaaaatt ggtgtaccac tagaagaagc agaaaaatgg
     9361 tacgccgctt taggacttcc ggtaattcca cccgatccca aacaccgcac accttctccg
     9421 attgtcgaag ttgaaccgca aggtagcggt ctgtatgtta ttattcccaa ccctcagatt
     9481 attgacagta tgagtcagtc ttcagactca atggtgcata gggacgataa agggaaggaa
     9541 aaaaatatat ctaaagaatt caccggttat gaaataagca cagctgaata ccaggcatgg
     9601 ctggcgggtt ataacggcca ggcagaaaac atgaaaaccg acgtgcaggt tatcacgact
     9661 aaatacagta cagccaactc cacctacgat acaattatca aattattatc ttcaaccatt
     9721 actgcgttgt ttgattcagc gaaagattat ttacgtttct gattaagtaa cacccacaat
     9781 tatcgcctac tatctggtcc cgaaaaatgg gaccagcgtt tcatattatt aagcatctgc
     9841 tttcattcat ttcttcatca caatcttaca aaatgagcgt tttttgatag agcaattctg
     9901 ccaatacttt attttatatt aatcaaatta atactatgaa tagagaaaac ggaaggagcg
     9961 ttctatgcaa ctgcaacatg gaaaaaataa tacacaacaa tatatatttg gcgactttgt
    10021 attaaaaaac aatggaatat tattattcaa aaacaaagaa tatcatattc ccccgaaaga
    10081 acttggcgtt atcattctat tacttaatgc tgacggtgaa attgttagca aagaagagat
    10141 tatcgataag gtctggagcg caagtgttgc cagtgatgaa tctctaacac gatgtattta
    10201 tgcattgcgc aaacttttgc atgagaataa acagtgtaag tatatcgaaa cggtctatgg
    10261 tcgtggatac cgttttactg tacctattgt gattgtgacc gataatgaac cggtaaagtc
    10321 gaccaccact cttgccgttt ttccctttcg tactgaaggt tcgatcaatg ttctaaaact
    10381 ccattacgaa cttgtccagg gcctttcaaa atatgctttt tgtggtttag atattttacc
    10441 cgcttcggta acaaatgagg ccagtgattt ctccaccatc catcagttta tcaataaaac
    10501 aggtccagaa tattatatta tgggtcaggt cgttcactat ggtaaaaact ggcgtttatt
    10561 tattgagtta atccatgcca gaacgcataa attgattgat catcaaagta ttgatttcaa
    10621 ccccgataat cctctttccg tattattatc acaagtaatt aatattctta ttgaaaaaat
    10681 acccaacatt aattttaaat ctattaatac acaacaaatg ccatctctgg attctgctgt
    10741 tatgtatatg aatggcagga tggaaatgta ccgttataca ccagaaagtt tgaaacgagc
    10801 attagtaata tttagagatt gtgtgagtac gcaaccgcaa aataccttgc cctactgttg
    10861 cctggcggaa tgccatatat cattagcact ccttggaatc agtgaacaaa aacaagcaat
    10921 aactacggca gtaacctcca ttgaaaaggc tctggaaatt aacccttcaa attctcaggc
    10981 attgggttta ctgggtttaa tcagtgggct aaaagatgaa cattcagtgt ctaatgtgct
    11041 atttaagcaa gcacatttac tcaaacctaa ttcgccagac gtttattatt atcagtcgct
    11101 gctatatttt ttgaacggcg accttgcaag agcatttaat ttaatagaaa aaagcattgc
    11161 tcttgaaccc aataaaatgg gcataagcat tcttaaatta attatacttt attacacatc
    11221 ccccctcgat aacgcgatat cttttgcatt aaatcttaac agtcaaaaca cctgtaataa
    11281 tccaattatt acctctattc aggctatgtt catggcactg aaaggacata atgacaaagc
    11341 caaaagttta ttgcttaaac ttgagcctga acatggtctt gattacacct gcgttaactc
    11401 gctttatacg aagtttctga tctatggcgc atctataaaa aatgacatta tgaagttact
    11461 ggcaaatatt aacactaaca aaatcaatgg tgtgattttg ccgttaattt acactgttta
    11521 tggtaagaaa gaatatgaaa agcgctggca acaattaatt aaagagaatg atttatggtc
    11581 aaatgttttg cttcatgacc cccgtttgct cagtgtaaaa aatgaattta atactattgg
    11641 tgtgatgcgt acttctgcgt aataacgaat ataaattgcc ctgctgatgc agggcaattt
    11701 agtgattact tcgttaataa tactctacgc acaagccaga ggactttcct gttccaagct
    11761 ctgatatacc agtttcagga ttaaatttct cgtgtactcc catgagctga gtgcttgtcc
    11821 aataatccgt accgctaaaa gcgttcccat aagtttttaa attaccatat tcgccccaca
    11881 acgtacctac ttttcgcggt actaccccac cgcttggccg ctgcgctaat tctaaagatg
    11941 acggcatttg accataacta catgaagtat ttatgatatc ccaggtatta ctactggtag
    12001 cacctaaact agtaaaccat tttttaatct tgaatttaaa tacaacatca ttccctttac
    12061 cattcttggg tttcccggta atagtaactt cacttcttat cagcacagat aatgttactt
    12121 ctccattact gtcaacatta attccataaa tcccagccgt ccaattataa tccgtatttt
    12181 tcatattatg ggttagtaac agcgtgaatt ttgcaccatc aaaccctgtt tctggaaaac
    12241 catctgtcgc cctgaatttt acaccattaa cactcagcaa ctcgacttcc acaaaaggaa
    12301 ggatggtata ggatggttgt ccgtatatac gccaaccttt aatctccata aatgaagtta
    12361 aattttctcc ttcttggtag gccatatatg tacgttcata tatgccatca cccacatatc
    12421 tccatttttc cccttctttt ttttctacat tttgtaagta cactgtatca tccagcaaat
    12481 ccagaccgtt atccaccgga tttccaaaat catcaactaa taaaacactg acaggtaaat
    12541 ccgccccttc cactactaaa acttctcgta tacgaagctg tgaaccaagc ggactcactt
    12601 cacctggttt gatagtgtaa ttgtttgaat aattggcatc gctccaacca gccatcttca
    12661 gttgcgcatt caaattcgct tccgcaaaat gcgccatata ctgcctttga tattgcccat
    12721 tgccgttgta aacccaatta ttgccctgga taggatccgc attcctgact tgtacgtttt
    12781 cctcattaag ctgaacgaca ccatcagtaa taaagttacc ctgggcgtct ttaagaacaa
    12841 cggtcaccgt tatgctatcc ccagcgatat atatcgtttt gtccgcgaca atcgttgatt
    12901 gagtttttgc cgctgcacct ggttgtatcc cgtatagcga ggacttaatc tcctctgccc
    12961 atgtttttaa tttcagactg gcaaccagat tttcctcggc aatccatgct gtccagacaa
    13021 tggtatattc accattattc tgctcgaccc accgcataga atcgggagtt gcgccgccta
    13081 ccgcgaaact atcaatataa gactcgatga cttctgatgt cagccctaaa gcagggttac
    13141 caaactcatc ccgcaacgtt attgtgacgg ttaatggctc cccggcgata tatgccgatt
    13201 tatctgtcct gatagcagaa tttgcctgca ccggagcacc gctgtggatg gaataactct
    13261 ctgattgttt actgctgccc cattcagaca acttcagcgt cgcgtggtga gagtctcccg
    13321 ccatttgcgc cgaccatgta gcagtataaa caccggcggt ttcagaccat cctcctgaac
    13381 gtacggctcc ttctactgtt acgttatcac cagacaataa ggactcgccc ccttcaacca
    13441 ggttaccgtg cgcgtctttc agcgtcacag cgacggttat agtgtcacca gcggtgtagg
    13501 cgtttttatc cgtagcgata gttgatgtgg cttgcgccgg agaaccggac tgtatgtcat
    13561 agcggtctga cgttacgcca tcactccacg tctgcaattg caggttggcg tgctgattgg
    13621 tgctggcaat cgtcgccatt cgggttgtgg tatacacgcc acctgattca acccagcttc
    13681 ccgtttctga cgggtcagtg ccagccaccg tcagcacacc atcagcggct aactgtgaag
    13741 ctgcaccggt taccagatta tcgaacgcat cccggagcgt taccgtgatg gtgattgtct
    13801 ctccaaccgt atagttcgac ttatttgttt caacggttga agtgagctgc gatgcatcac
    13861 caggtacaaa ggtgacgttc tggctctggc tgctgttgtt gatgctggcg gagactgtac
    13921 tggtgcccgc cgtctggctg gtgacactga tctccaccgt gccgtccggc tttgtggtca
    13981 ccgtcgaagc aaccgttgcg ctgttgcccg ccaacacgct gaccgtctgt ccggcaagcg
    14041 tattaccgtt agcatcggtg accttcacct gcagcgtatt cgccgtcgaa ccgtctgcca
    14101 ccgcgttatc ccggatgacc tccagttcag cgatcttcgc tgtcctgaca tcggcgataa
    14161 atgtcacgtc ccggctctga ctgctgctgt tgatgctggc agtgactgca ctgattcccg
    14221 ccgtctggct ggtgacactg atctccaccg tgccatccgg ctgagtggtg accgccggag
    14281 cgaccgttgc gccgttacct gccatcacgc tgaccgtctg cccggcaagc gcattaccga
    14341 atgcatcggt taccctcgcc cgcagcgtgt tcgccgtcgc accgtctgcc tctgaaccgt
    14401 ccttaatgac caccagatcc gcgatctgcg ccgtgctgat atcggcgata aacgtcacat
    14461 cccggctctg gctgctgttg ttgatgctgg cggtgactgc actgactccc gccgtctggc
    14521 tggtgacact gatctccgcc gtgccgtccg gttgcgtggt gaccgtcgga gcgaccgttg
    14581 cgccattgcc tgccgtcacg ctgaccgtct gcccggcaag cgcattaccg aatgcatcgg
    14641 tcaccctcgc ccgtagcgtg ttcgccgtcg caccgtccgc ctctgaaccg tccttaatga
    14701 ccaccagatc cgcgatctgc gccgtcctga catcggcgat aaacgtcacg tttcggctct
    14761 ggctgctgtt gttgatgctg gcggtgaccg cactgattcc ggcggtctgg ctggtgacag
    14821 agatctccac cgtcccgtcc ggctgcgtgg tgaccgtcgg agcagtcgtg gcaccgttgc
    14881 ctgccaacac gctgaccgtc tgtccggcaa gcgcattacc gaacgcatcg gtgaccctcg
    14941 cccgcaacat attcgccatt acaccgtctg ccgctgagcc gtccttaatg accaccagct
    15001 ccgcgatctg cgccgtcctg acatcggcga taaatgtcac gttctgactc tgactactgc
    15061 tgttgatggt tgcagtgact gtactgatcc ccgccgtctg gctggtgaca ctgatttcca
    15121 ccgtgccgtc ctgccctgtg atgaccgtcg gagcgaccgt tgcgccgtta tccgccaaca
    15181 cgctgaccgt ctgcccggca agtgcattgc cgaacgcatc ggtcaccctc gcccgcagcg
    15241 tgttcgccgt cgcaccgtcc gcctctgaac catccttaat gaccaccaga tcagcgatct
    15301 gcgccgtcct gacatcggcg ataaacatca cgctctggct caggctatgg ttgttgatgg
    15361 ttgctgtgac tgtactgatc cctgccgtct ggctggtgac tgagatatcc accgtaccgt
    15421 ccggctcagt gatgaccgcc ggagcaaccg ttgcgctgtt gtctgccaac acgctgaccg
    15481 tctgtccggc aagcgcattc ccgaacgcgt cggtgaccct cgcctgcagc gtattcgccg
    15541 tcgcaccgtc tgccgctgag ccgtccttaa tgaccaccag ctccgcgatc tgcgccgtcc
    15601 tgacatccgc aataaacgtc acatcccggc tcaggctgct gttgttgata ctggcggtga
    15661 ccgcactggc tcccgccgtc tgactggtga cagggatctc caccgtgcca tccggctgag
    15721 tggtgaccgc cggagcgacc gttgcgccgt tacctgccat cacgctgacc gcctgcccgg
    15781 caagcgtatt accgttagca tcggtgaccc tcacctgcag cgtattcgcc attgcgccgt
    15841 cagccacggc gttatcctgc gtcacctcca gactcgcgat ctgcgccgtc ctgatatcgg
    15901 cgataaacgt cacatcccgg ctctggctgc tgttgttgat gctggcggtg actgcactga
    15961 ctcccgccgt ctggctggtg acactgatct ccgccgtgcc gtccggctct gtaatcaccg
    16021 ttggggcaac cgttgcgccg ttgcctgcca tcacgctgac cgtctgcccg gcaagcgtat
    16081 taccgaacgc atcggtcacc ctcgcccgca gcgtgttcgc cgtcgaaccg tctgccaccg
    16141 cgttatcctg gctaacgacc agatccgcta tcttcgccgt cctgacatcc gcgacaaacg
    16201 tcacgttctg actctggctg ctgttgttga tgctggcggt gaccacactg attcctgccg
    16261 tctggctggt gacactgatc tccaccgtgc cgtccggctt tgtggtcatc gtcgaagcga
    16321 ccgctgcacc gttgtccgcc atcacgctga ccgtctgccc ggcaagcgca ttaccgaaca
    16381 catcggagac cctcgcccgc agcatattcg ccattgcacc gtctgccact gagtcgtcct
    16441 taatgaccac caggtccgcg atctgcgccg ttctgacatc cgcgataaat gtcacgtccc
    16501 ggctctgact gctgctgttg atactgacgg tgaccgcact gctgcccgcc gtctggctgg
    16561 tgacactgat ttccaccgtg ccgtccggct gcgtggtgac caccggagcg accgttgcgc
    16621 cgttgtccgc ca
//
LOCUS       DAMZFT010000111        16502 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.111, whole genome shotgun sequence.
ACCESSION   DAMZFT010000111 DAMZFT010000000
VERSION     DAMZFT010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16502)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16502)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16502
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.111"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(<1..845)
                     /locus_tag="PN977_003695"
     CDS             complement(<1..845)
                     /locus_tag="PN977_003695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HDI6043694.1"
                     /translation="MKLPVREFDAVVIGAGGAGMRAALQISQSGQTCALLSKVFPTRS
                     HTVSAQGGITVALGNTHEDNWEWHMYDTVKGSDYIGDQDAIEYMCKTGPEAILELEHM
                     GLPFSRLDDGRIYQRPFGGQSKNFGGEQAARTAAAADRTGHALLHTLYQQNLKNHTTI
                     FSEWYALDLVKNQDGAVVGCTALCIETGEVVYFKARATVLATGGAGRIYQSTTNAHIN
                     TGDGVGMAIRAGVPVQDMEMWQFHPTGIAGAGVLVTEGCRGEGGYLLNKHGERFMERY
                     APNAK"
     gene            complement(845..1192)
                     /gene="sdhD"
                     /locus_tag="PN977_003696"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
     CDS             complement(845..1192)
                     /gene="sdhD"
                     /locus_tag="PN977_003696"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase membrane anchor subunit"
                     /protein_id="HDI6043695.1"
                     /translation="MVSNASALGRNGVHDFILVRATAIVLTLYIIYMVGFFATSGELT
                     YEVWIGFFASAFTKVFTLLALFSILIHAWIGMWQVLTDYVKPLALRLMLQLVIVVALV
                     VYVIYGFVVVWGV"
     gene            complement(1186..1590)
                     /gene="sdhC"
                     /locus_tag="PN977_003697"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
     CDS             complement(1186..1590)
                     /gene="sdhC"
                     /locus_tag="PN977_003697"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000263334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HDI6043696.1"
                     /translation="MWALFMIRNVKKQRPVNLDLQTIRFPVTAIASILHRVSGVITFV
                     AVGILLWLLGTSLSSPEGFEQASAIMGSFFVKFIMWGILTALAYHVVVGIRHMMMDFG
                     YLEETFEAGKRSAKISFVITVVLSLLAGVLVW"
     gene            1971..2078
                     /locus_tag="PN977_003698"
     CDS             1971..2078
                     /locus_tag="PN977_003698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6043697.1"
                     /translation="MRKSYEVGISPKINLCNSVEVLTNSFGTVISGRQV"
     gene            2284..3567
                     /gene="gltA"
                     /locus_tag="PN977_003699"
     CDS             2284..3567
                     /gene="gltA"
                     /locus_tag="PN977_003699"
                     /EC_number="2.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (Si)-synthase"
                     /protein_id="HDI6043698.1"
                     /translation="MADTKAKLTLNGDTAVELDVLKGTLGQDVIDIRTLGSKGVFTFD
                     PGFTSTASCESKITFIDGDEGILLHRGFPIDQLATDSNYLEVCYILLNGEKPTQEQYD
                     EFKTTVTRHTMIHEQITRLFHAFRRDSHPMAVMCGITGALAAFYHDSLDVNNPRHREI
                     AAFRLLSKMPTMAAMCYKYSIGQPFVYPRNDLSYAGNFLNMMFSTPCEPYEVNPILER
                     AMDRILILHADHEQNASTSTVRTAGSSGANPFACIAAGIASLWGPAHGGANEAALKML
                     EEISSVKHIPEFVRRAKDKNDSFRLMGFGHRVYKNYDPRATVMRETCHEVLKELGTKD
                     DLLEVAMELENIALNDPYFIEKKLYPNVDFYSGIILKAMGIPSSMFTVIFAMARTVGW
                     IAHWSEMHSDGMKIARPRQLYTGYEKRDFKSDIKR"
     gene            3714..4088
                     /locus_tag="PN977_003700"
     CDS             3714..4088
                     /locus_tag="PN977_003700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001519567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gybgO"
                     /protein_id="HDI6043699.1"
                     /translation="MIDFGKFNLLDIRRHTMSKTFSINTTKSQNDQCTDGFKVSSSFY
                     TEETLVEEDKALLIGNSLKLRLLDENASPYTFNKYAEYADFTSDMLIYEKTYTAELSS
                     TPGTLIAAGPFDTVVLFKINYN"
     gene            4240..5286
                     /locus_tag="PN977_003701"
     CDS             4240..5286
                     /locus_tag="PN977_003701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415243.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HDI6043700.1"
                     /translation="MPVLQWGMLCVLSLLLSIGFLGLHLPAALLLGPMLAGIIFSMRG
                     ITLQLPRSAFLAAQAILGCMIAQNLTGSILTTLAVNWPIVLAILLVTLLSSAIVGWLL
                     VRYSSLPGNTGAWGSSPGGAAAMVAMAQDYGADIRLVAFMQYLRVLFVAGAAVLVTRM
                     MLGDNAEAVNQQIVWFPPVSINLLLTILLAVVAGTAGCLLRLPSGTMLIPMLAGAVLQ
                     SGQLITIELPEWLLAMAYMAIGWRIGLGFDKQILLRALRPLPQILLSIFALLAICAGM
                     AWGLTRFMHIDFMTAYLATSPGGLDTVAVIAAGSNADMALIMAMQTLRLFSILLTGPA
                     IARFISTYAPKRSA"
     gene            complement(5283..6074)
                     /gene="nei"
                     /locus_tag="PN977_003702"
     CDS             complement(5283..6074)
                     /gene="nei"
                     /locus_tag="PN977_003702"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease VIII"
                     /protein_id="HDI6043701.1"
                     /translation="MPEGPEIRRAADNLEAAIKGKPLTDVWFAFPQLKSYQSRLIGQH
                     VTHVETRGKALLTHFSNDLTLYSHNQLYGVWRVVETGEEPQTTRVLRVKLQTADKTIL
                     LYSASDIEMLTPEQLTTHPFLQRVGPDVLDPKLTPEVAKERLLSPRFRNRQFAGLLLD
                     QAFLAGLGNYLRVEILWQVGLTGNHKAKDLNAAQLDALAHALLDIPRLSYATRGQVDE
                     NKYHGALFRFKVFHRDGEPCERCGGIIEKTTLSSRPFYWCPGCQH"
     gene            complement(6110..6844)
                     /gene="pxpA"
                     /locus_tag="PN977_003703"
     CDS             complement(6110..6844)
                     /gene="pxpA"
                     /locus_tag="PN977_003703"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HDI6043702.1"
                     /translation="MKIDLNADLGEGYASDAELLTLVSSANIACGFHAGDAQTMQACV
                     REAIKNGVAIGAHPSFPDRENFGRSAMQLPPETAFAQTLYQIGALAAIARAQGGVMRH
                     VKPHGMLYNQAAKEAQLADAIARAVYACDPALILVGLAGSELIRAGKQYGLTTREEVF
                     ADRGYQADGSLVPRSQPGALIEDEEQALAQTLEMVQHGRVKSITGEWATVTAQTVCLH
                     GDGEHALAFARRLRATFAEKGIVVAA"
     gene            complement(6834..7766)
                     /gene="pxpC"
                     /locus_tag="PN977_003704"
     CDS             complement(6834..7766)
                     /gene="pxpC"
                     /locus_tag="PN977_003704"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpC"
                     /protein_id="HDI6043703.1"
                     /translation="MLKIIRAGMYTTVQDGGRHGFRQSGISHCGALDMPALRIANLLV
                     GNDANAPALEITLGQLTVEFETDGWFALTGAGCEARLDDNAVWTGWRLPMKAGQRLTL
                     KRPQHGMRSYLAVAGGIDVPAVMGSCSTDLKVGIGGLEGRLLKDGDRLPIGKAKRDFM
                     EAQGVKQLLWGNRIRALPGPEYHEFDRTSQDAFWRSPWQLSSQSNRMGYRLQGQILKR
                     TTDRELLSHGLLPGVVQVPHNGQPIVLMNDAQTTGGYPRIACIIEADMYHLAQIPLGQ
                     PIHFVQCSLEEALKARQDQQRYFEQLAWRLHNEN"
     gene            complement(7760..8416)
                     /gene="pxpB"
                     /locus_tag="PN977_003705"
     CDS             complement(7760..8416)
                     /gene="pxpB"
                     /locus_tag="PN977_003705"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HDI6043704.1"
                     /translation="MQRARCYLIGETAVVLELEPPVTLASQKRIWRLAQRLVDMPNVV
                     EAIPGMNNITVILRNPESLALDAIERLQRWWEESEALEPESRFIEIPVVYGGAGGPDL
                     AVVAAHCGLSEKQVVELHSSVDYVVWFLGFQPGFPYLGSLPEQLHTPRRAEPRLLVPA
                     GSVGIGGPQTGVYPLATPGGWQLIGHTSLSLFDPARDEPILLRPGDSVRFVPQKEGVC
                     "
     gene            complement(8439..9182)
                     /gene="ybgI"
                     /locus_tag="PN977_003706"
     CDS             complement(8439..9182)
                     /gene="ybgI"
                     /locus_tag="PN977_003706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000798886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radiation resistance protein YbgI"
                     /protein_id="HDI6043705.1"
                     /translation="MKNTELEQLINEKLNSAAISDYAPNGLQVEGKETVQKIVTGVTA
                     SQALLDEAVRLGADAVIVHHGYFWKGESPVIRGMKRNRLKTLLANDINLYGWHLPLDA
                     HPELGNNAQLAALLGITVMGEIEPLVPWGELTMPVPGLELASWIEARLGRKPLWCGDT
                     GPEVVQRVAWCTGGGQSFIDSAARFGVDAFITGEVSEQTIHSAREQGLHFYAAGHHAT
                     ERGGIRALSEWLNENTDLDVTFIDIPNPA"
     gene            9453..10934
                     /gene="dtpD"
                     /locus_tag="PN977_003707"
     CDS             9453..10934
                     /gene="dtpD"
                     /locus_tag="PN977_003707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide permease DtpD"
                     /protein_id="HDI6043706.1"
                     /translation="MNKHASQPRAIYYVVALQIWEYFSFYGMRALLILYLTNQLKYND
                     THAYELFSAYCSLVYVTPILGGFLADKVLGNRMAVMLGALLMAIGHVVLGASEIHPSF
                     LYLSLAIIVCGYGLFKSNVSCLLGELYEPTDPRRDGGFSLMYAAGNVGSIIAPIACGF
                     AQEEYSWAMGFGLAAVGMIAGLVIFLCGNRHFSHTRGVNKKVLRATNFLLPNWGWLLV
                     LLVATPALITVLFWKEWSVYALIVATIIGLGVLAKIYRKAENQKQRKELGLIVTLTFF
                     SMLFWAFAQQGGSSISLYIDRFVNRDMFGYTVPTAMFQSINAFAVMLCGVFLAWVVKE
                     SVAGNRTVRIWGKFALGLGLMSAGFCILTLSARWSAMYGHSSLPLMVLGLAVMGFAEL
                     FIDPVAMSQITRIEIPGVTGVLTGIYMLLSGAIANYLAGVIADQTSQASFDASGAINY
                     SINAYIEVFDQITWGALACVGVVLMIWLYQALKFRNRALALES"
     gene            complement(10976..12394)
                     /gene="phrB"
                     /locus_tag="PN977_003708"
     CDS             complement(10976..12394)
                     /gene="phrB"
                     /locus_tag="PN977_003708"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HDI6043707.1"
                     /translation="MTTHLVWFRQDLRQHDNLALAAACRNSSVRVLALYIATPRQWAA
                     HNMSPRQAEFINAQLNGLQIALAEKGIPLLFHEVDDFAASVEIVKQVCAENRVTHLFY
                     NYQYEVNERARDVQAERALRNVVCEGFDDGVILPPGAVMTGNHEMYKVFTPFKNAWLK
                     RLREGMPECVAAPKVRSSGSIDPAPSITLNYPRQPFDTAHFPVEEKAAIAQLRQFCQN
                     GAGEYEQQRDFPAVEGTSRLSASLATGGLSPRQCLHRLLAEQPQALDGGAGSIWLNEL
                     IWREFYRHLITYYPSLCKHRPFIVWTDRVQWQSNSAHLKAWQEGKTGYPIVDAAMRQL
                     NSTGWMHNRLRMITASFLVKDLLIDWREGERYFMSQLIDGDLAANNGGWQWAASTGTD
                     AAPYFRIFNPTTQGEKFDREGEFIRQWLPELRDVPGKVVHEPWKWAQKAGVTLDYPQP
                     IVDHKEARLRTLAAYEEARKGA"
     gene            complement(12391..12900)
                     /locus_tag="PN977_003709"
     CDS             complement(12391..12900)
                     /locus_tag="PN977_003709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgA family protein"
                     /protein_id="HDI6043708.1"
                     /translation="MNQQLFDDSTLIRIFALHELHNLKDRGLTRGALLDYHSRYKLVF
                     LAHSQPEYRKLGPFVADIHQWQNLDDYYNQYRQRVIVLLSHPANRRDHTNVLMHVQGY
                     FRPHIDSTERQQLAALIDSYRRGEQPLLAPLMRIKHYMALYPDAWLSVQRYFELWPCV
                     INLRHSGVL"
     gene            complement(13001..13207)
                     /locus_tag="PN977_003710"
     CDS             complement(13001..13207)
                     /locus_tag="PN977_003710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbfA family protein"
                     /protein_id="HDI6043709.1"
                     /translation="MELYREYPAWLIFLRRTYAVAAGVLALPFMLFWKDRARFYSYLH
                     RVWSKTSEKPVWMDQAEKATGDFY"
     gene            13520..13609
                     /gene="kdpF"
                     /locus_tag="PN977_003711"
     CDS             13520..13609
                     /gene="kdpF"
                     /locus_tag="PN977_003711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit F"
                     /protein_id="HDI6043710.1"
                     /translation="MSAGVIAGVLLVFLLLGYLVYALINAEAF"
     gene            13609..15282
                     /gene="kdpA"
                     /locus_tag="PN977_003712"
     CDS             13609..15282
                     /gene="kdpA"
                     /locus_tag="PN977_003712"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HDI6043711.1"
                     /translation="MAAQGFLLIATFLLVLMVLARPLGSGLARLINDIPLPGTTGVER
                     VLFSALGVSDREMNWKQYLSAILGLNMLGLAVLFFMLLGQHYLPLNPQQLPGLSWDLA
                     LNTAVSFVTNTNWQSYSGETTLSYFSQMAGLTVQNFLSAASGIAVIFAFIRAFTRQSM
                     STLGNAWVDLLRITLWVLVPVALLIALFFIQQGALQNFLPYQAVNTVEGAKQLLPMGP
                     VASQEAIKMLGTNGGGFFNANSSHPFENPTTLTNFVQMLAIFLIPTALCFAFGEVAGD
                     RRQGRMLLWAMSVIFVICVGVVMWAEVQGNPHLLALGADSSINMEGKESRFGVLVSSL
                     FAVVTTAASCGAVIAMHDSFTALGGMVPMWLMQIGEVVFGGVGSGLYGMMLFVLLAVF
                     IAGLMIGRTPEYLGKKIDVREMKLTALAILVTPTLVLMGAALAMMTDAGRSAMLNPGP
                     HGFSEVLYAVSSAANNNGSAFAGLSANSPFWNCLLAFCMFVGRFGVIIPVMAIAGSLV
                     SKKSQPASSGTLPTHGPLFVGLLIGTVLLVGALTFIPALALGPVAEYLS"
     gene            15305..>16502
                     /gene="kdpB"
                     /locus_tag="PN977_003713"
     CDS             15305..>16502
                     /gene="kdpB"
                     /locus_tag="PN977_003713"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
                     /protein_id="HDI6043712.1"
                     /translation="MSRKQLALFEPTLVVQALKEAVKKLNPQAQWRNPVMFIVWIGSL
                     LTTFISIAMASGAMPGNALFSAAISAWLWVTVLFANFAEALAEGRSKAQANSLKGVKK
                     TAFARKLREPKYGAAADKVPADQLRKGDIVLVEAGDIIPCDGEVIEGGASVDESAITG
                     ESAPVIRESGGDFASVTGGTRILSDWLVIECSVNPGETFLDRMIAMVEGAQRRKTPNE
                     IALTILLIALTIVFLLATATLWPFSAWGGNAVSVTVLVALLVCLIPTTIGGLLSAIGV
                     AGMSRMLGANVIATSGRAVEAAGDVDVLLLDKTGTITLGNRQASEFIPAQGVDEKTLA
                     DAAQLASLADETPEGRSIVILAKQRFNLRERDVQSLHATFVPFTAQSRMSGINIDNRM
                     IRKGSVD"
BASE COUNT         3781 a         4424 c         4218 g         4079 t
ORIGIN      
        1 tctttggcgt tcggcgcata acgctccata aagcgttcgc catgtttgtt cagcagataa
       61 ccgccttcac cacggcaacc ttcggtgacc agtacgcccg caccggcaat accggtcggg
      121 tggaactgcc acatttccat atcctgtacc ggtacgcctg cacggatagc catgccgaca
      181 ccgtcgccag tgttaatgtg ggcgttggtg gtggactgat aaatacgccc tgccccgcca
      241 gtcgccagca cggtagcgcg agctttgaag taaaccactt caccagtttc gatgcacagt
      301 gcggtacaac cgaccaccgc gccatcctgg tttttcacca gatccagcgc ataccactcg
      361 gagaaaatgg tggtgtggtt tttcaggttt tgctgataaa gggtgtgcaa cagtgcgtga
      421 ccggtacggt cagcagccgc cgcagtacgt gccgcctgct cgccgccgaa gtttttcgac
      481 tgaccgccaa acggacgttg atagatacga ccatcatcaa gacgcgaaaa cggcaggccc
      541 atgtgttcga gttccagaat cgcttccggc ccggttttac acatatattc aatcgcgtcc
      601 tggtcaccga tatagtccga cccttttacg gtatcgtaca tatgccattc ccagttatct
      661 tcatgggtat tacccagcgc aacggtaatg ccgccttgcg cagaaacggt atgggaacgg
      721 gtcgggaaga ctttagagag cagcgcacag gtctgaccgc tctgggaaat ttgcagcgcc
      781 gcgcgcatac ctgcgccacc ggcaccaatc acaactgcat caaattctct gactggcaat
      841 ttcatcacac accccacacc acaacgaatc cataaatcac gtaaaccacc agtgcaacga
      901 caatcaccag ttgcagcatc aggcgcaagg ccagcggttt aacgtagtcg gtcaacacct
      961 gccacatgcc gatccaggca tgaatcaaga tagaaaacag cgccagcagg gtgaacactt
     1021 tggtgaacgc agaggcgaag aaaccaatcc agacttcata tgtcagctcg ccactggtag
     1081 cgaaaaaacc gaccatataa atgatgtaga gcgtcaggac gatagcggta gcgcgaacga
     1141 ggatgaaatc atgtacgcca ttgcgtccta atgcggaggc gttgcttacc atacgaggac
     1201 tcctgcgaga agtgaaagca cgacagtaat aacaaaggag attttggcgg agcgtttacc
     1261 cgcttcgaat gtttcttcca gatagccaaa atccatcatc atgtggcgaa tacctacgac
     1321 gacgtgatac gccagagcgg taaggatgcc ccacatgata aatttgacga agaagctgcc
     1381 cataatcgca gaagcttgct cgaaaccttc aggggaagag aggctggtac ccagaagcca
     1441 cagcaggatg cccactgcaa caaaggtgat cacaccggaa acgcgatgga gaatggacgc
     1501 tatcgccgtg acggggaacc ggatggtctg taggtccaga ttaacaggtc tttgtttttt
     1561 cacatttctt atcatgaata acgcccacat gctgttctta ttattccctg gggactacgg
     1621 gcacagaggt taactttctg ttaccaggag acgtcgggat ttccttcctc cggtctgctt
     1681 gcgggtcaga cagcgtcctt tctataactg cgcgtcatgc aaaacactgc ttccagatgc
     1741 gaaaacgaca cgttacaacg ctgggtggct cgggattgca gggtgttccg gagacctggc
     1801 ggcagtatag gccgttcaca aaatcattac aattaaccta catatagttt gtcgggtttt
     1861 atcctgaaca gtgatccagg tcacgataac aacatttatt taatttttaa tcatctaatt
     1921 tgacaatcat tcaacaaagt tgttacaaac attaccagga aaagcatata atgcgtaaaa
     1981 gttatgaagt cggtatttca cctaagatta acttatgtaa cagtgtggaa gtattgacca
     2041 attcattcgg gacagttatt agtggtagac aagtttaata attcggattg ctaagtactt
     2101 gattcgccat ttattcgtca tcaatggatc ctttacctgc aagcgcccag agctctgtac
     2161 ccaggttttc ccctctttca cagagcggcg agccaaataa caaacgggta aagccaggtt
     2221 gatgtgcgaa ggcaaattta agttccggca gtcttacgca ataaggcgct aaggagacct
     2281 taaatggctg atacaaaagc aaaactcacc ctcaacgggg acacagctgt tgaactggat
     2341 gtgctgaaag gcacgctggg tcaagatgtt attgatatcc gtactctcgg ttcaaaaggt
     2401 gtgttcacct ttgacccagg cttcacttca accgcatcct gcgaatctaa aattactttt
     2461 attgatggtg atgaaggtat tttgctgcac cgcggtttcc cgatcgatca gctggcgacc
     2521 gattctaact acctggaagt ttgttacatc ctgctgaatg gtgaaaaacc aactcaggaa
     2581 cagtatgacg aatttaaaac tacggtgacc cgtcatacca tgatccacga gcagattacc
     2641 cgtctgttcc atgctttccg tcgcgactcg catccaatgg cagtcatgtg tggtattacc
     2701 ggcgcgctgg cggcgttcta tcacgactcg ctggatgtta acaatcctcg tcatcgtgaa
     2761 attgccgcgt tccgcctgct gtcgaaaatg ccgaccatgg ccgcgatgtg ttacaagtat
     2821 tccattggtc agccattcgt ttacccgcgc aacgatctct cctatgccgg taacttcctg
     2881 aatatgatgt tctccacgcc gtgcgaaccg tatgaagtta atccgattct ggaacgtgct
     2941 atggaccgta ttctgatcct gcacgctgac catgaacaga acgcctctac ctccaccgtg
     3001 cgtaccgctg gctcttcggg ggcgaacccg tttgcctgta tcgcagcagg tattgcttca
     3061 ctgtggggac ctgcgcacgg tggtgctaac gaagcggcgc tgaaaatgct ggaagaaatc
     3121 agctccgtta aacacattcc ggaatttgtt cgtcgtgcga aagacaaaaa tgactctttc
     3181 cgcctgatgg gcttcgggca ccgcgtgtac aaaaattacg acccgcgcgc caccgtaatg
     3241 cgtgaaacct gccatgaagt gctgaaagag ctgggtacca aagatgacct gctggaagtg
     3301 gctatggagc tggaaaacat cgcgctgaac gacccgtact ttatcgagaa gaaactctac
     3361 ccgaacgtcg atttctactc tggtatcatc ctgaaagcga tgggtattcc gtcttctatg
     3421 ttcaccgtga tcttcgcgat ggcgcgtacc gttggctgga tcgcccactg gagcgaaatg
     3481 cacagtgacg gtatgaagat tgcccgtccg cgtcagctgt atacaggata tgaaaaacgc
     3541 gactttaaaa gcgatatcaa gcgttaatgg ttgattgcta agccgttcac tttccggacc
     3601 cgcctttaat agagcgggtt ctttttatat ggctgaacat aaaaaattgt aaaaattaaa
     3661 ccagatgcaa atacccatta ataaggtgaa aaaatatttc accggaaaca caggtgattg
     3721 attttggtaa atttaatctg ctggatattc gccgccacac aatgtcgaaa acattcagta
     3781 ttaacacgac aaaatcacaa aatgaccaat gcaccgacgg atttaaagtc agttcctcct
     3841 tttataccga agaaacgttg gttgaagaag ataaagcatt gttgattggt aatagtctga
     3901 agctacgttt gctggatgaa aacgcctcgc cttatacatt caataaatat gctgaatacg
     3961 ccgatttcac cagtgatatg ctgatctacg aaaaaaccta tacggctgaa ttatcgtcca
     4021 cacctggcac tctcatcgcg gcgggtccct tcgatacagt ggtacttttt aagattaact
     4081 ataactgatt taacacgacg cccttttcag ccggaaaggg caacgtattc agattattgc
     4141 tgattttccc cttttccttg attaccgtta ttttttccag acccagcttt ctttatgctg
     4201 tatcgataca ccaaagagaa aataatgagg aagcgaagga tgccagtttt gcagtgggga
     4261 atgttatgtg tgctctcact tctactttct attggcttcc tcggattaca tctcccggcg
     4321 gcgctattac ttgggccgat gctcgccggg atcatcttca gtatgcgcgg aattacttta
     4381 caactccccc gctccgcttt tctcgccgcg caggccattc ttggctgcat gattgcgcaa
     4441 aacctgaccg gttcgatcct gaccacgcta gcggtgaact ggccgattgt gttagctatt
     4501 ttactggtga cgctgctttc cagcgccatc gtgggttggt tattggtgcg ctatagctca
     4561 ttgcctggaa ataccggtgc ctggggttct tctcctggcg gcgcggcggc aatggtcgct
     4621 atggcgcaag attacggcgc agatattcgc ctggtggcgt ttatgcaata tctgcgcgtg
     4681 ctgttcgtcg cgggggccgc tgttctggtc acccgcatga tgctgggcga taacgctgaa
     4741 gcggttaacc agcagatcgt ctggttcccg ccagtgagca ttaatctcct gcttacgatt
     4801 ttgctcgcgg tcgttgccgg tacggcggga tgcttgctac gtcttccttc cggcacgatg
     4861 ctcatcccga tgctggcggg cgcagtgctc cagtctggtc agctcatcac catcgaacta
     4921 ccagaatggc tgctggcgat ggcgtatatg gcaattggct ggcggattgg tcttggtttc
     4981 gataagcaaa tcttactgcg ggcattgcgc ccgctgccac aaatcttgct gtcgattttt
     5041 gctctgctgg ctatttgtgc aggtatggcg tggggactga cccgatttat gcatattgat
     5101 tttatgactg cctacctcgc caccagtccc ggcgggcttg ataccgtggc ggtcatcgcc
     5161 gcagggagca atgccgatat ggcgctcatc atggcgatgc aaaccctgcg cctgttcagt
     5221 attttgctaa cggggcctgc catcgcacgg tttatttcaa cctatgcgcc gaagcgttcg
     5281 gcctagtgct ggcagccagg gcaccagtaa aacgggcgcg atgacagcgt ggttttctca
     5341 atgatgccgc cacagcgttc gcaaggttcg ccatcacgat gaaaaacctt aaagcgaaac
     5401 agcgccccat gatatttgtt ttcatccacc tgcccccgcg tagcgtaaga aagtcgcgga
     5461 atatccagta acgcgtgtgc gagtgcgtcc agttgcgccg cattgagatc tttcgcttta
     5521 tgatttccgg tcaacccaac ctgccagagg atctccaccc gcaaataatt gccaagtcct
     5581 gccagaaacg cctgatcgag cagtaatcca gcaaactgac ggttacgaaa gcgcggcgac
     5641 aataaccgtt ctttcgccac ctccggcgtc agtttcggat ccagcacatc gggaccaacg
     5701 cgttgtaaaa acggatgcgt ggtcagttgt tccggggtca acatctcaat atccgaggcg
     5761 ctataaagca gaatggtttt gtcagccgtt tgcagtttta cccgcaatac tcgcgtggtc
     5821 tgcggctctt cgccggtttc aaccacgcgc cagacgccgt aaagctgatt atggctgtag
     5881 agcgttaagt cgttggaaaa atgagttaac aacgccttac cacgcgtttc cacatgggta
     5941 acgtgttgac cgataagtcg tgattgataa gattttaact gcgggaaggc aaaccagaca
     6001 tcagttagtg gtttgccttt gatcgccgcc tccaggttat ccgctgcacg gcggatctcc
     6061 gggccttcag gcatggtgat atcctttttt cattctttat taagcggggt tatgctgcga
     6121 caacaatccc cttttcagca aatgtagctc gtaatcggcg ggcgaacgcc agtgcgtgct
     6181 cgccatcgcc atgcaggcag accgtttgcg ccgtgaccgt tgcccattcg ccggtgatac
     6241 ttttgactct gccgtgttgc accatctcca gcgtttgcgc aagcgcctgt tcttcgtctt
     6301 caatcagcgc gcctggctgg cttcgcggca ccagcgagcc gtcagcctgg taaccgcgat
     6361 cggcaaacac ttcctcgcgc gttgtcaggc catattgctt gcctgcacga atcagctcgc
     6421 ttcccgccag cccgacgaga atcaatgccg gatcgcaagc gtataccgct ctggcgatgg
     6481 cgtctgccag ttgtgcctct ttcgccgcct ggttgtataa catgccgtgc ggtttgacat
     6541 gacgcattac gccgccttgc gcacgggcaa tcgctgccag cgcgccaatt tgatacagcg
     6601 tctgggcgaa agcggtttct ggcggcagct gcatggcgct gcgaccaaaa ttttccctgt
     6661 cgggaaaact cgggtgagcg ccaatcgcaa caccattttt tattgcttca cgcacgcaag
     6721 cctgcatggt ttgcgcatcg cctgcatgaa atccacaggc aatattggca gaggaaacca
     6781 gcgttaatag ctctgcgtcg ctggcatagc cttcgcccag atcggcgttc aggtcaattt
     6841 tcattgtgca gccgccacgc taattgttcg aaataacgtt gctgatcttg ccgtgctttc
     6901 agtgcttctt ccagtgaaca ctggacaaaa tgaatcggct gaccgagcgg aatttgcgcc
     6961 agatggtaca tatcagcctc gatgatacag gcaatacgcg gataaccacc ggtggtctgt
     7021 gcgtcgttca tcaacacaat gggctgcccg ttatgcggca cctgcaccac gcccggtaac
     7081 aaaccgtgag ataacagttc gcgatcggtg gtgcgtttta aaatttgccc ctgtaagcga
     7141 tagcccatgc ggttactttg cgagctaagc tgccagggcg aacgccagaa tgcatcctgc
     7201 gaggtgcgat cgaactcatg atattccggc cccggcaagg cgcgaatgcg gttgccccac
     7261 agcagctgtt taacgccctg cgcttccata aaatcacgct tcgctttgcc aatcgggaga
     7321 cggtcaccat ccttcagtaa acggccttcc agcccaccaa tccccacttt gagatcggta
     7381 ctgcatgacc ccattaccgc cggaacatca ataccacccg cgaccgccag ataactgcgc
     7441 atcccgtgct gcgggcgttt aagcgttaaa cgctggcctg ctttcatcgg caatcgccag
     7501 ccggtccaga cggcgttatc atccagccgc gcttcgcaac cggcccccgt cagagcaaac
     7561 cacccatcag tttcgaactc aaccgttaac tgaccgagcg tgatctccag cgcgggggca
     7621 ttggcgtcat tacccaccag caggttagca atgcgcagcg caggcatatc cagtgcgccg
     7681 cagtggctga tacccgactg gcgaaaaccg tgacgaccgc catcctgcac agtggtatac
     7741 atgcccgcac gaataatctt cagcatactc cctccttctg cggtacaaag cgcacgctgt
     7801 ctcccggacg taataagatg ggttcgtcac gcgccggatc aaacaggctg agtgaggtat
     7861 gaccaatcaa ctgccagcca cccggcgttg ccagcggata aacaccagtc tgcggcccgc
     7921 cgatcccgac agaacctgca ggaacgagca agcgcggttc agcgcgccgt ggcgtgtgta
     7981 gttgttccgg caaactcccg agatacggga agcccggttg aaaacctaaa aaccagacca
     8041 cgtagtccac ggaggagtgc aattcaacaa cctgtttctc actcaaccca caatgcgccg
     8101 cgaccactgc taaatccggt ccgcctgcac caccgtaaac caccggaatt tcaataaagc
     8161 gagactccgg ctccagcgcc tcactctcct cccaccagcg ttgcaaacgc tctatggcat
     8221 ccagcgccag cgactcagga ttacgcaaaa tcaccgtgat attgttcatg ccgggaatag
     8281 cttcaaccac attcggcata tccaccagac gctgtgccaa tcgccagatc cgtttctggc
     8341 tagccagcgt caccggcggt tccagctcca gcactaccgc cgtttcacct atcagataac
     8401 aacgcgctcg ttgcactttc gatccctctg attattcgtt atgcaggatt aggaatatca
     8461 ataaaggtca catcaagatc ggtattttca ttcagccact cgctcaacgc gcgaatacca
     8521 ccacgttcag tggcatggtg acccgcagca taaaaatgca atccctgctc gcgggctgaa
     8581 tgaatggtct gttcagaaac ttcgccagta ataaaagcat ccacgccaaa acgcgcggcg
     8641 ctatcgataa aactttgccc gccgcccgtg caccaggcaa cgcgctggac cacctcaggt
     8701 ccggtatcgc cacaccataa cggcttacgt cccagacgcg cttcaatcca ggaggccagt
     8761 tccagtcccg gcacaggcat ggtcagttcg ccccacggca ccaacggctc aatttcgccc
     8821 atgaccgtga tccccagtaa cgccgccagt tgtgcgttat tacccagctc cggatgcgcg
     8881 tccaggggca gatgccagcc atacaggttg atatcattcg ccagcaacgt ttttaaacgg
     8941 ttacgcttca ttccgcgaat gaccggagat tcacctttcc agaagtagcc atgatgcaca
     9001 atgaccgcat ccgcgcccag acgcactgcc tcatcgagca aagcctggct ggcagtgaca
     9061 ccggtaacaa ttttttgcac cgtctctttg ccttccacct gcaaaccatt cggcgcgtaa
     9121 tcactaatcg ccgcgctgtt cagtttttcg ttaatcagtt gttccagttc ggtgtttttc
     9181 atcttcgcgc cttccgtatt ctgatagcta caccgttaac atagcgttac ctcgcctgtt
     9241 cgtcgatagc caatcttttg acatattaag aaacaaaccc gacgccaaaa gacgcaaatt
     9301 gtttatatca tcacgcttca gctaatgtat atttatgcaa tcagacagca tattgcataa
     9361 ataatcagat ctgtatcaaa gttttaactt taaggaaaca caagcacgcc gcgcaactat
     9421 tacgtgacgt tgcagtcaat tgtggaatta ttatgaataa acacgcatca cagccgcgcg
     9481 ctatttacta tgtcgttgcg ctgcaaatct gggaatattt tagcttttac ggcatgcgtg
     9541 ccctgctgat tctctatctc accaatcaac taaaatacaa cgatactcac gcctacgagt
     9601 tatttagcgc ctactgttcg ctggtgtatg tcacgccaat cctcggtggc tttttggcgg
     9661 ataaagttct cggcaatcgc atggcggtga tgctgggggc gttgttgatg gcgatcggtc
     9721 atgtggtgct gggtgccagt gagatccatc cgtcattcct ctatctgtca ctggcgatta
     9781 tcgtctgcgg ctatggtctg tttaaatcca acgtcagttg cctgctcggt gagctgtatg
     9841 agccaaccga tccacgtcgt gatggcggtt tctcgctgat gtatgcggcg ggtaacgtgg
     9901 ggtctattat cgcacctatc gcctgtggtt tcgcccagga agaatacagt tgggcgatgg
     9961 gctttggcct ggcggcggtg ggtatgatcg cgggtctggt cattttctta tgtggcaatc
    10021 gtcatttctc tcatacccgc ggcgttaaca aaaaggtact gcgtgcgaca aacttcctcc
    10081 tgcccaactg gggatggctg ctggttctgc tggtggcaac gcctgcgctg attaccgtgt
    10141 tgttctggaa agagtggtcg gtatacgcct tgattgtcgc cacgatcatc ggccttggcg
    10201 ttctggcaaa aatttatcgc aaagcagaaa accaaaaaca gcgtaaggag ctggggctga
    10261 ttgttaccct caccttcttc agcatgttgt tctgggcctt cgcacaacag ggcggcagct
    10321 cgattagcct ttatatcgac cgcttcgtta accgcgatat gtttggttat accgttccga
    10381 ctgctatgtt ccagtcgatt aatgccttcg cagttatgct gtgcggtgtg ttcctggcgt
    10441 gggtggttaa agagagcgtc gcaggtaatc gtaccgtgcg catctgggga aaatttgctc
    10501 ttggtctcgg cctgatgagc gccggattct gcattctgac cttaagcgcc cgctggtccg
    10561 caatgtatgg tcattcttct ctgccactga tggtgttagg cctggcggtg atgggctttg
    10621 cggaactgtt tatcgacccg gttgctatgt cgcagattac gcgcattgaa atccctggcg
    10681 tgactggcgt attaactggc atttatatgc tgctttccgg cgcgattgcg aactatctgg
    10741 ctggcgttat tgccgatcag acatcgcaag cttcgtttga tgcttccggg gcgatcaact
    10801 attccatcaa tgcatatatt gaagtgtttg atcaaattac ctggggcgca ctggcgtgtg
    10861 taggagtggt gttgatgatt tggctgtatc aggcgctgaa attcagaaac cgcgcgctgg
    10921 cgctggagtc ttaataggcc agcaccatac ctgataaaac gctcgctgcg ttttatcagg
    10981 ctcctttcct cgcttcttcg tacgctgcca gcgttcgcag ccttgcctct ttgtgatcaa
    11041 ctatcggttg cggataatcc agcgtcacac ctgctttctg cgcccacttc cacggctcat
    11101 gcaccacttt ccctggcaca tcgcgcagtt ccggtagcca ctggcggata aactcgcctt
    11161 cgcggtcaaa tttctcgccc tgggttgtcg ggttaaaaat acgaaaatac ggcgctgcat
    11221 cggttccggt tgaagcggcc cactgccagc caccgttatt ggctgccaaa tcaccatcaa
    11281 tcagctgcga catgaaatat cgctcacctt cgcgccagtc gatcaataaa tctttcacca
    11341 aaaaactggc tgtaatcatc cgtagccggt tatgcatcca gccagtgctg ttaagctgac
    11401 gcatggcagc atcaacaatc gggtatcccg ttttgccttc ctgccaggcc tttaaatgcg
    11461 cggaattgct ctgccactgc acgcggtcgg tccagacaat aaacggacga tgtttacaca
    11521 gcgaggggta atacgttatc agatgacggt aaaactcgcg ccagatcagc tcattaagcc
    11581 agatactacc ggccccaccg tccagcgcct gcggctgttc cgccaacagg cgatgcaaac
    11641 actggcgagg cgataacccg cccgttgcca ggctggcgga caaacggctg gtgccttcta
    11701 ccgcagggaa atctcgctgt tgctcatact ctccggcacc gttctggcaa aactggcgta
    11761 attgcgcaat cgccgctttc tcttccaccg ggaaatgcgc agtatcgaaa ggctgacgag
    11821 gataattcag cgtaatggat ggcgcgggat ctatcgatcc gctactacgt acttttggcg
    11881 cagcgacgca ctccggcatc ccttctcgca gccgtttaag ccaggcgttt ttaaatggcg
    11941 taaagacttt gtacatctca tgattgccag tcatcaccgc ccccggcggc aggatcacgc
    12001 cgtcgtcaaa tccttcacac accacgttac gcagcgccct ctcagcctgc acatcccgcg
    12061 cccgctcatt cacctcatac tgatagttat aaaacagatg ggtaacacga ttttccgcgc
    12121 acacctgttt aactatttcg acgctggcgg caaagtcatc cacttcatga aacagcaaag
    12181 gaatgccttt ttccgccagc gcgatttgta gcccgttcag ttgtgcattg ataaattcag
    12241 cctgacgcgg cgacatatta tgcgccgccc actggcgtgg ggtagcgata tacaacgcca
    12301 gcacgcgcac agacgaattg cggcaggcgg cagccagcgc gagattatcg tgctgacgta
    12361 aatcctggcg aaaccagacc agatgggtag tcataaaact cctgaatggc gcaagttaat
    12421 cacacacggc caaagttcga aataacgctg cactgaaagc caggcgtcag gataaagcgc
    12481 catatagtgt ttgatacgca tcagcggcgc aagaagtggt tgctcgccac ggcgataact
    12541 gtcgataagc gcagccagct gctggcgttc tgtggaatca atatgagggc gaaaataacc
    12601 ctgaacgtgc atcaaaacgt tagtatgatc acgccggttg gcagggtgag aaagcaaaac
    12661 aattacgcgt tggcggtact ggttgtaata gtcatccaga ttttgccact ggtgaatatc
    12721 agccacaaac gggccaagtt tgcggtactc gggctgggaa tgggccagaa agacgagttt
    12781 atagcggctg tgataatcga gaagcgcccc gcgcgttagt cctcgatctt tgagattgtg
    12841 taactcatgc aaagcaaaaa tgcggataag ggtgctgtca tcaaaaagtt gttgattcat
    12901 tactgctcct gaacggttta caggagtaag tgtagacgcc gaaaaaagaa aaccgccagc
    12961 gtttccgctg gcggttttct ttgaacgtaa tatttagcaa tcagtaaaaa tcacccgttg
    13021 ccttttcggc ctgatccatc cacaccggtt tctcgctggt tttcgaccag acgcggtgca
    13081 ggtagctgta aaagcgggcg cggtctttcc agaaaagcat gaaaggcagc gccagaacgc
    13141 ccgctgcaac cgcataagta cggcgtaaaa agataagcca tgcaggatat tctctgtaga
    13201 gttccatact tttctccccc ttttgtatct acccggtgaa tggcaccgga aaaatgaatt
    13261 tgtttatctg atgaaaatag taccgccttt tgtgtaattt tactactcat ccgaccactt
    13321 atttttgttt attgatggtt tatttacatt cactgtgtaa ttaagttaca caaaagttaa
    13381 attaatacta accattagtt aaatcatggc ttttgccatt tttatacttt ttttacaccc
    13441 cgcccgcaga tttttgcgaa atctttgcag ccagaattct acccttccga tatcactttt
    13501 aggccactgg aggtgcactg tgagtgcagg cgtgatagcc ggcgtgttgc tggtgttttt
    13561 attactgggt tatctggttt atgccctgat caatgcggag gcgttctgat ggctgcgcaa
    13621 gggttcttac tgattgccac gtttttactg gtgttaatgg tgctggcacg tcctttaggc
    13681 agcgggctgg cgcggctgat taatgatatt cctcttcctg gcacgacggg cgttgaacgc
    13741 gtactcttta gcgcgcttgg cgtctctgac cgcgagatga actggaagca gtatctctct
    13801 gccattctcg gcctgaacat gctggggctg gcggtgctgt tttttatgtt gctcggtcag
    13861 cactatctgc cgcttaatcc acagcagttg ccagggctgt catgggatct ggcgctgaat
    13921 accgccgtca gctttgtcac caataccaac tggcaatctt atagcggtga aaccacgttg
    13981 agctatttca gccagatggc gggcttaacg gtgcaaaact ttctttctgc cgccagcggg
    14041 attgcggtga tttttgcctt catccgtgca tttacccgcc agagcatgag cacactcggg
    14101 aatgcctggg tcgatctgct acgcatcacg ttatgggtgc tagtccctgt ggcgttgttg
    14161 atcgcactgt tttttattca acaaggtgcg ctgcaaaact ttctgcccta tcaggcggtg
    14221 aataccgttg aaggagcgaa acagctgtta ccaatgggtc cggtggcttc tcaggaagcg
    14281 atcaagatgc tcggcactaa cggtggtggc ttctttaatg ccaactcgtc gcatccattt
    14341 gaaaacccaa ccacactgac caacttcgtg cagatgctgg cgatcttctt gatcccaaca
    14401 gcgctgtgct ttgcctttgg tgaagtggcg ggcgatcgcc gccaggggcg catgttgctg
    14461 tgggcgatgt cagtgatttt tgtcatctgc gtaggcgtag tgatgtgggc agaagttcag
    14521 ggtaatcctc atctgctggc gctcggcgcg gacagcagca tcaatatgga aggtaaagag
    14581 agccgtttcg gcgtgctggt cagtagcctg tttgcggtcg tgactacggc ggcttcctgc
    14641 ggcgcggtga ttgcgatgca tgattcgttt accgctcttg gtggcatggt gccgatgtgg
    14701 ttgatgcaaa ttggtgaagt ggtattcggc ggcgtcggtt ccggccttta cggcatgatg
    14761 ctgtttgtcc tgctggcggt gtttattgcc gggctgatga ttggtcgtac accggaatat
    14821 ctgggtaaaa aaatcgacgt acgcgagatg aaactgactg cactggcgat tctggtcacc
    14881 ccgacgctgg tgctgatggg cgcggctctg gcaatgatga ccgacgccgg acgtagcgcc
    14941 atgctcaacc ctggcccgca tggttttagc gaagtgctgt acgccgtgtc gtccgccgct
    15001 aacaacaacg gcagcgcctt tgccggatta agcgccaact ctccgttctg gaactgttta
    15061 ctggcgttct gcatgtttgt cggtcgcttc ggggtgatta tcccggtgat ggcgattgcc
    15121 ggttcgctgg tgagtaaaaa gagccagccc gccagctccg gtacactgcc tacgcacggc
    15181 ccgctgtttg ttggcctgtt aatcggcacc gtgttgctgg ttggcgcact gacctttatc
    15241 cctgccctgg cgcttggtcc ggtggcggaa tatctctcct gatgatattg agtgagcact
    15301 gaatatgagt cgtaaacaac tggcgctatt cgaaccaaca cttgtcgttc aggcgctgaa
    15361 agaagcggtg aaaaaattaa acccgcaggc gcaatggcgc aatccggtga tgtttatcgt
    15421 ctggatcggc agtctgctga ccacctttat tagcatcgca atggcaagcg gcgcgatgcc
    15481 tggcaatgcg ctgtttagcg cggcaattag cgcatggctg tgggtcaccg tactgttcgc
    15541 taatttcgcc gaggcgctgg ctgaaggccg cagtaaagcg caggccaaca gtctgaaagg
    15601 tgtaaaaaaa accgcctttg cccgcaagct gcgtgagccg aaatatggcg ctgcggcgga
    15661 caaagtacct gccgatcaac tgcgtaaagg cgatatcgta ctggtagaag ctggcgatat
    15721 tatcccctgc gatggtgaag ttattgaagg gggggcatcg gtcgatgaaa gcgccatcac
    15781 cggggaatcg gcaccggtga tccgtgaatc cggcggcgat tttgcctccg tcactggcgg
    15841 tacgcgtatt ctttctgact ggctggtgat tgagtgcagc gttaaccccg gcgagacatt
    15901 tctggatcgg atgatcgcga tggtggaagg cgcgcagcga cgcaaaacgc caaacgagat
    15961 tgccctgacc attctcctga ttgccctgac tatcgtcttt ttactggcaa ccgccacgct
    16021 gtggccgttt tccgcgtggg gcggtaatgc agtcagcgta acggtactgg tggcgctgct
    16081 ggtctgtctg atcccaacga ccattggcgg tctgttgtca gcgatcggcg tcgccgggat
    16141 gagtcggatg ctgggcgcga atgtgattgc caccagcgga cgtgcagttg aagcggcagg
    16201 tgacgttgac gttctgctac tggataaaac cggcaccatc acacttggta accgtcaggc
    16261 gtcggagttt atccctgcgc agggcgtgga tgaaaaaacg ctggctgacg ccgcacaact
    16321 ggcttcgctg gctgatgaaa cgccggaagg ccgcagtatt gtgatcctcg ccaagcagcg
    16381 ttttaacctg cgtgagcgcg atgtgcagtc gcttcacgcc acctttgtac cgtttactgc
    16441 gcaaagccgg atgagcggga tcaacatcga caaccgcatg atccgtaaag gttctgtcga
    16501 tg
//
LOCUS       DAMZFT010000112        16155 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.112, whole genome shotgun sequence.
ACCESSION   DAMZFT010000112 DAMZFT010000000
VERSION     DAMZFT010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16155)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16155)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16155
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.112"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(214..1506)
                     /locus_tag="PN977_003714"
     CDS             complement(214..1506)
                     /locus_tag="PN977_003714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007373071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDI6043713.1"
                     /translation="MAHRYLTRQDHRTLSLAALGGALEFYDFIIFVFFAAVIAELFFP
                     PDIPEWLRQLQAFGIFAAGYLARPLGGIILAHFGDILGRKKMFTLSILLMAVPTLVIG
                     ILPTYSSVGTLAPVLLLLMRLLQGAAIGGEVPGAWVFVAEHVPERRTGIACGTLTAGL
                     TAGILLGSVVATLVNSVLSQDIVYDYGWRFPFLLGGVFGLAAMYLRRWLEETPVFLEM
                     KARKKLAQELPVKAVVLRHKKAVVISMLLTWLISAGIIVVILMAPVWLQKQYDFSPTV
                     TLQANSIATIMLSLGCLLAGMAIDSFGAGKTFISGSILLGCSGWLFYHTVGEYPEMLF
                     WLYGLAGFSVGIVGAVPYVLVRIFPAEVRFTGISFSYNVAYAVVGGLTPVVVTMLTEI
                     SPMAPAWYVMLLSLVGSGLGIWFCRDPRLFICHADKDK"
     gene            complement(1682..3325)
                     /gene="lldP"
                     /locus_tag="PN977_003715"
     CDS             complement(1682..3325)
                     /gene="lldP"
                     /locus_tag="PN977_003715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HDI6043714.1"
                     /translation="MDIWSQDYDPVGNIWLSSLTASLPILFFFFALIKLKLKGHVAAT
                     WTVIIALIVALLFYEMPLTAALASVLYGFFYGLWPIAWIIIAAVFVYKISVKTGQFDI
                     IRASVLSVTPDQRIQMLIVGFAFGAFLEGAAGFGAPVAITAALLVVLGFRPLYAAGLC
                     LIVNTAPVAFGAMGIPILVAGQVTGLDSFEIGQMVGRQLPFLTIIVLFWIMAIMDGLR
                     GIKETWPAVLVAGGSFAIAQFLSSNFLGPELPDIISSLVSLVCLTLFLKRWQPVHIFR
                     FDNTPYQNTDSSPLSRSQVIRAWSPFLFLTATVTLWSLPPFKTLFAPGGTLHDLVITV
                     PVPYLDKLIARMPPVVSEATAYAAVFKFDWFSATGTAILFAALLSVIWLKMKPSDAVS
                     TFGSTLKELALPVYSIGMVLAFAFISNYSGLSSTLALALAQTGSAFTFFSPFLGWLGV
                     FLTGSDTSSNALFASLQATAAHQIGVSDILMVAANTTGGVTGKMISPQSMAIACAATG
                     LVGKESELFRFTVKHSLIFTCIVGVITSLQAWVFTWMIP"
     gene            4207..4497
                     /locus_tag="PN977_003716"
     CDS             4207..4497
                     /locus_tag="PN977_003716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001523382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDI6043715.1"
                     /translation="MGIYHSRVAGIYDTPGSMSDAHFEILAALSPVNSKKVILALRDY
                     FVLGLTRREACENHDVAQGYFSVSLRKLLETNRLVAELAELLSVPSECRPYW"
     gene            complement(4574..5740)
                     /gene="lldD"
                     /locus_tag="PN977_003717"
     CDS             complement(4574..5740)
                     /gene="lldD"
                     /locus_tag="PN977_003717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent L-lactate dehydrogenase LldD"
                     /protein_id="HDI6043716.1"
                     /translation="MIISSPADYRDAAKHRLPRFLFDYIDGGAVTEKTMHENMTSLSS
                     VSLRQQVLCGAGAPELNTTILDSEWAMPVALGPVGATGMYARRGEVQAARAASAMNIP
                     YTLSTVSVCPIEEVAGHADGQLWSQLYVLRDRGYMQNALERAWTAGMKTLVFTVDMPV
                     PGSRYRDNHSGMSGPHATLRQYLQAVLHPRWALDVGIAGRPLSFGNIETYTGHKMSMD
                     DYMGFISKNFDPSIAWNDLEWIRDGWKGKLIIKGILVPEDAKNAVRLGADGIIVSNHG
                     GRQLDGAIPTARALPAIADAVGDDITVLADSGIRSGVDVVRMLALGAKGVLLGRAYIY
                     ALAAAGKKGVEHLLRLYAEDMKVTMTLIGASSPADISSRNLEYLEKEFSRHSSS"
     gene            complement(6300..7544)
                     /locus_tag="PN977_003718"
     CDS             complement(6300..7544)
                     /locus_tag="PN977_003718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDI6043717.1"
                     /translation="MSENATFSPEQSCRKHTAAPAKSLSFVEGVSMIVGTNIGAGVLS
                     VAYASGKAGFLPLLFWLVLVGSLTTITMLYVAESTLRTRKHLQLSGLSHRYVGRCGAL
                     IMFLSVCVNSVGALIAYMSGSGKLLHSLLGISPALGSMLFFIPAAGVLYLGLKAIGRG
                     EKFISIGMVVMLTILIVATMLKDTTQMRYLLDGNWLYMVPVFNVVAFCFSAQYIVPEM
                     ARGFSDKPEKLPKAIMVGMGITFILLALVPMSVIALNGLDNISDVATISWGRALGEWA
                     FFSANLFALCAMLTSYWGLGGSFLTNIVDQFSPGDDSQPLRRFLLLLIVVIPPFILAY
                     SGMVSFVNALYFAGVFSGVVLSVMPILILKGARQHGDLTPGWTCPALIIHPLLQSFIV
                     LLYLCSAAYAIASALGHLPAGW"
     gene            complement(7679..8944)
                     /locus_tag="PN977_003719"
     CDS             complement(7679..8944)
                     /locus_tag="PN977_003719"
                     /EC_number="2.6.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="HDI6043718.1"
                     /translation="MKNSELNHRRQQATPRGVGVMCDYFVGKAENATLWDVEGNEVID
                     FAAGIAVLNTGHRHPKIVAAVEQQLQSFTHTAYQIVPYESYVSLAERINAVAPVDGPA
                     KTAFFTTGAEAVENAVKIARAYTGRPGIITFGGGFHGRTFMTMALTGKVAPYKTGFGP
                     FPGSVYHAVYPNDAHGISVDDALRSLDRIFKADIPADQVAAIVLEPVQGEGGFNVAPA
                     AFMQALRELCDTHGILLVADEVQTGFARTGRLFAMQHYAVKADLITMAKSLAGGFPLS
                     GVVGRAEVMDAPAPGGLGGTYAGNPLAVAAAHAVLDIIEEEQLCQRADQLGEHLKEVL
                     SQARAGCPAIADVRGLGSMVAVEFNNPQSGEPSAEYARQVQHKARENGLLLLNCGIYG
                     NVIRFLYPLTIPDAQFSKALNILTRILRT"
     gene            9063..10556
                     /locus_tag="PN977_003720"
     CDS             9063..10556
                     /locus_tag="PN977_003720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016807578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HDI6043719.1"
                     /translation="MRAFIGDLLLFRMGQEQDKLLHKRLYNALRYAILDGSLPPHSRL
                     PASRELAVELGISRNTVLTTYEQLQAEGYVVSRRGSGTFVAQTAPDTFLMAFSNTEKS
                     EDARLSSAVSHRGDYLLRHVSASPCQWGAFIPGVPDVSAFPHPLFSRIQARISRRPEP
                     ERLSYSCTGGTPELQHALVDYLRIARGVRCQADQILITEGIHQAIDLVARMLADAGDM
                     AWVEEPSYWGIRHVLAMNAVQITPVAVDASGLVPPERLMPPPRLIFVTPSHQYPLGVV
                     MSLERRQRLLALARQHGSWIVEDDYDSEFRFSGQPVPALQGLIDDAPVVYIGTFSKTV
                     YPGLRLGYLVLPRPLKEALKRAHAELYRGGHTLIQMALAEFINGGHYSAHIRRMRLLY
                     SRRRAFLGELIERHCGTQALSDFSDNAGLHLILNLPDDVDDVAVAASANAQNILVRPL
                     SLYYLTAQRKRGLLMGFASLPEAQMEEAFATLLTCLRHHCPQVLVKV"
     gene            complement(10698..11633)
                     /locus_tag="PN977_003721"
     CDS             complement(10698..11633)
                     /locus_tag="PN977_003721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001349471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDI6043720.1"
                     /translation="MNITLRQIKYFVSTAESGSLSTAAKSLNISQSAITTAIQGLERD
                     IGYKLLIRSKSGVDLTDEGRLFLYNAYNIIRTYDEAINTVHVQKPLHGTVSIAATYTI
                     MGYFLPVHLKQFSRIYPHIKLELFENQRSIIETGLLSGKYDIGIVINDNLTCPDIVSE
                     TLLRSERRIWLPSGHPLLEKEILSLNDIVSEPFIILDVDEAWETAKRYWNTASTMPDV
                     ILHTHSIEAVRSMVANDMGITILSDMVYRPWSLEGKRIETRALSKSEVFPMSIGLAWR
                     NNVEFTPAMQAFINYFRKIFMCSPGYQNRNVDHFI"
     gene            11776..12537
                     /gene="pxpA"
                     /locus_tag="PN977_003722"
     CDS             11776..12537
                     /gene="pxpA"
                     /locus_tag="PN977_003722"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000946308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HDI6043721.1"
                     /translation="MLRSIDISSDMGESFGAWTLGDIRDIDIMPYISSANIATGFHAG
                     DPGIMHRTIISARDSGVSVGAHPGYRDLAGFGRRHIHCTAEEITNDMIYQLGALREFA
                     TLHNVPVNHVKPHGALYMHLAHDRDAAKHFVSTLHSYAPELRIYCMYGSAIWKEATQI
                     GHPVICEFYADRDYDQSGSIVFIRRTEPLNPDDVAKKVLRACLEGKVRTVEDQDIDIH
                     FSSVCVHSDTPGALSLLIAINKALREADISVNSQN"
     gene            12555..12800
                     /locus_tag="PN977_003723"
     CDS             12555..12800
                     /locus_tag="PN977_003723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012885088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxyl carrier domain-containing
                     protein"
                     /protein_id="HDI6043722.1"
                     /translation="MAVTEILSPLPGVFYRKPAPDSAPFLENGDTVQESTVIGLIEIM
                     KQFNEIHAETTGTNIQFKVENGEEVEPGQVLATIEII"
     gene            12811..14172
                     /locus_tag="PN977_003724"
     CDS             12811..14172
                     /locus_tag="PN977_003724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001354627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HDI6043723.1"
                     /translation="MSIQKLLIANRGEIALRIIRSAKKLNIKTVLACSESDINSLPAK
                     SADEVIVIGKSRADDSYLDGKKIIDAALQSGAQAIHPGYGFLSENASFARAVLDAGLI
                     FVGPSPQIIEQMGDKAIARDCAIKAGVPVVPGSNGEVISPEEALSVASDITGYPLLIK
                     ASAGGGGKGIRIVHSPEQLVHEYTVARAEAKAAFGHDGVYLERYIPEARHVEVQILGD
                     GKNIVHLFERECSLQRKRQKVTEEAPSPSVSASLREALCQSALNLARMLNYSSAGTIE
                     YLVDDRSGEFFFIEMNTRIQVEHPVTEMVTGIDIIDWMLRIAGGDSLSFGQDEITLQG
                     AAIEMRINAENPYNGFMPSPGTISVLQIPEGQGIRVDTHMFEGYKMPLWYDSLLAKLI
                     IHTDSREKTISLARETLGNMQIEGVHTTIPLHEALLSAEFFRNAQFHNKTLEDWLNTG
                     AFK"
     gene            14182..15066
                     /locus_tag="PN977_003725"
     CDS             14182..15066
                     /locus_tag="PN977_003725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000129813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyltransferase domain-containing protein"
                     /protein_id="HDI6043724.1"
                     /translation="MTDINLRYTFGADEHLFIEVSEEMSLKAFFHSLSLTRKIESLNI
                     NGVLDICLANASCQIRFNPDIIHPDDLLQQIRNVNVLSGNGEMTLQTRIIEIPVLYND
                     PWTHETLMRFRNNHQNPALTDLEYAAEINGYPDVQTFIEAHHSSPWFVSMVGFVAGLP
                     FMYQMVDRKKQIQVPKYLVPRTDTPKHSVGHGGCFGAIYPVRGAGGYQLFGITPAPIY
                     DTEQKLSYFKDSMVFFRAGDIIKFRAINTEQYNQCISDVESGNFELLIRPVNFDLNAF
                     EENPEDYTKNLLGELYGN"
     gene            15056..16042
                     /locus_tag="PN977_003726"
     CDS             15056..16042
                     /locus_tag="PN977_003726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000511730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-dependent carboxyltransferase"
                     /protein_id="HDI6043725.1"
                     /translation="MGIKVLTPGLSTSVQDGGREGFYHLGIPPSGALDRTGLVTANLL
                     VGNHPFDAALECTLLGPELLFEQDTLFAVSGAPFTVTLDNQSVPVNTSLLARKGQILK
                     IAYTTVGVRCYLAIAGGINVPVIAGSRSTYLLGSLGGYKGRRLQAGDELPVQEAICNK
                     GRINYTLPDELRPVFTKENELRVVPGMYIHRLKQESVESFFDDVWKISKDSDRTGFRF
                     QGGRSLNFVDRHPPFGAGSDPSNIVDACYPVGSIQVPSGSTPIVLHRDAVSGGGYMTV
                     GTVVSTDLDILAQLAPGSSVTFSQVSMDKALEIRKEFNERLNLLINSLSYGS"
BASE COUNT         4424 a         3703 c         3970 g         4058 t
ORIGIN      
        1 acgagcccgg aaacgtgtca ggacgatttc accggaacat gacggcggac cactacctgg
       61 tgatgtcgcg tggcgctatc atccggcagg gcaaggggga ggatatggag actgatgggg
      121 ggcgcggtct ggtgagcatc tgaccggatg gcgacaggtt aacctgtcgc catttttaaa
      181 aactgaatga taccgttata taacggtgaa acattatttg tctttgtctg cgtgacagat
      241 aaaaagacgg ggatccctgc agaaccatat tcccagtcct gatcccacaa gtgataacag
      301 cataacatac catgctggag ccatcgggga gatttccgtt aacatcgtga ccacaacagg
      361 agtcagtccc cccaccactg cataagcgac attgtaagag aatgaaatgc cggtaaacct
      421 gacctctgcg gggaaaatac gcacgagaac ataagggacg gccccgacaa tgcccacact
      481 gaatcccgcc agcccataca gccagaacaa catttcagga tactcaccga cagtgtgata
      541 gaacagccat cctgaacatc caagcagaat actgcctgat ataaaggttt ttccggcacc
      601 aaaagaatca atggccatac cggccagcag gcatcccaga ctcagcataa tagttgcaat
      661 gctgttagcc tgtaaggtga ctgtcggcga aaagtcatac tgtttctgta accagaccgg
      721 tgccatcagt atcaccacaa taatccctgc ggatatcagc catgtcaaca gcatggaaat
      781 cacaacagct tttttatgtc ttaaaacgac agcttttacc ggtaattcct gagccagttt
      841 ttttctggct ttcatttcca gaaataccgg agtttcctcc agccagcggc gcaggtacat
      901 tgctgccagg ccaaaaacac caccaagaag gaaggggaat cgccagccgt aatcgtaaac
      961 aatatcctga gacaatactg aattgacaag tgtggcaacc actgaaccaa gcaggatccc
     1021 ggcggttaaa cctgctgtca gtgttccgca tgcaataccg gttcggcgct ccggtacatg
     1081 ctcagcaaca aaaacccagg caccgggcac ttcaccgcca atcgctgcgc cctgaagcag
     1141 acgcatcaga agaagcagca caggagccag ggtacccaca gagctgtagg ttggcaaaat
     1201 accgataaca agtgtcggta ctgccataag caaaatactc agggtgaaca ttttctttcg
     1261 cccaagtata tcgccaaaat gggccagaat tattccgccg agaggtcgtg ccaggtaccc
     1321 ggcagcaaat ataccaaatg cctggagctg acggagccat tccggtatat ccggcgggaa
     1381 aaagagctca gcaatgacgg cggcgaaaaa aacaaaaatg atgaagtcat agaattcaag
     1441 tgcgcctccg agcgcagcca gagataaggt cctgtgatcc tgtcgtgtca ggtagcggtg
     1501 tgccatataa acatacctgt cagttgaata ttgaatgtga aaccctgtcg tcagtatatc
     1561 cgtgacttaa gaaaaccaga atatccggca gtcacgtcag ccagcactac tgcagagtgt
     1621 tataagccag aggctgtaac acacatcatc atgccggaaa agatccggca caacaaatat
     1681 ttcatgggat catccaggtg aatacccatg cctggagaga ggtgatgact cccacaatac
     1741 aggtgaagat caggctgtgt ttaacagtaa aacgaaagag ttctgattct ttgccaacaa
     1801 gtccggttgc tgcgcaggcg atggccatgg actgtggaga aatcattttg ccggttactc
     1861 cccctgtggt gttggcggca accatcagaa tatcggagac cccgatctga tgagctgccg
     1921 ttgcctggag tgatgcaaat aatgcattgg atgaggtatc tgatcccgtc agaaatacgc
     1981 ccagccagcc cagaaacggt gagaagaacg taaaggcgct gcctgtctgt gccagagcca
     2041 gggcaagtgt ggatgaaagc cccgaataat tagagataaa ggcgaaagcc agcaccatgc
     2101 cgatggaata gacgggcagg gcaagttctt tcagtgtact gccaaaggtg ctgacagcat
     2161 ctgacggttt catcttcagc cagatcacag agagcaatgc tgcaaacagg atggcggtac
     2221 cggtggcaga gaaccagtca aacttaaaca ccgcggcata agcagtggct tcgctgacta
     2281 ccggaggcat acgtgcaatg agtttatcaa gatacggcac aggaacagta atcaccaggt
     2341 catgcagtgt accgcccggt gcgaacagtg ttttaaaggg aggaagactc cagagtgtca
     2401 ccgtggcagt cagaaacaga aatggcgacc atgcccgtat cacctgtgaa cggctcagtg
     2461 gtgatgagtc cgtattctgg tacggggtgt tgtcaaagcg aaaaatatgt acaggctgcc
     2521 agcgtttcag gaaaagcgtc agacaaacca gcgacaccag tgatgatatg atgtccggga
     2581 gctccgggcc cagaaagttg gaactgagaa actgggctat ggcgaaggaa cctccggcaa
     2641 ccagaacggc gggccaggtt tccttaatgc cacgcaggcc atccataatt gccatgatcc
     2701 agaacaacac aataatggtc aggaagggta actgacgacc aaccatctga ccgatttcaa
     2761 agctgtccag cccggtaacc tggcctgcga cgagaatggg gatccccatc gcaccaaagg
     2821 ccacgggagc tgtattgacg atcagacaca acccggccgc gtaaagtggt ctgaacccaa
     2881 gcaccacgag cagtgctgct gttatggcca caggtgctcc aaaaccggcg gccccttcga
     2941 gaaaggcacc aaaggcaaac cccacaatca gcatctgaat acgctgatca ggcgtaacgg
     3001 acagcactga agcgcgaatg atatcgaact gtccggtttt tacagaaatt ttataaacaa
     3061 aaactgcggc aataattatc caggcaatgg gccacaggcc atagaagaaa ccatagagca
     3121 cggaagcgag tgcggctgtg agaggcatct cataaaaaag aagggccacg atgagcgcga
     3181 taatgacagt ccatgtggca gcaacatggc ctttaagctt cagcttaatc agggcaaaga
     3241 aaaaaaacag aatgggaagc gatgctgtca ggctggacag ccagatattt ccgacgggat
     3301 catagtcctg tgaccagata tccatgaaca gacctcctgg tgaagtatgg tgtaatacct
     3361 gtacagcaga gagcgagtgc attgctgaca tgtccgtcag agtgtctgca tattgtttca
     3421 gtccggcaac gggaggaagg tcgtaatttc aggatttgtg aattatccgg agtaagggtg
     3481 taaatctggt agtaccacaa tctcatcgtc tgttttgttc cggatctttt taatgaaagg
     3541 tgaagaagga tcttatacga cggtatatac gtattgatta tatctttatt tatagaaatt
     3601 ataaaaaaat gaaaaaaagc cgtacaccac agacgtacgg cacacaaata cccaaattaa
     3661 ggaagttttc gttacaaggg cagttgcccg tgaatgttgt agcgtaataa tgccggattg
     3721 tgaagtgtta atttatcctg tatccggctg tctgcgcctg tttttctttg tgtggtctct
     3781 gtcatgtgcg gaatatcgca tttacgcctg aatgggtaag ccatgaaaat acattctgcc
     3841 tgaaccggag tgttgcagaa caatatttat caggctaagc aatttacttt atttaattaa
     3901 ataaatgatt ttcaggtggt gttttttgta aagataccaa aatttaaaaa aggtgttaaa
     3961 aatgcagtac ctggtcgttt ttgacggttt ttgtgacttt atgttctgtt tttgatggtt
     4021 gttctggttt taaatatcgt ttctatgcgt tattacaggc tctttctgtt ttcctgcggt
     4081 tgacccgacc ggttttttca gtattctgcg aataatccga aatataatat tatataaata
     4141 tacttatatc tcagcctgtt ctcagtctcc cggggaataa gggcaggcgg taatcagggg
     4201 gtatccgtgg ggatatatca cagtcgtgtt gccgggatat atgacacgcc cggaagcatg
     4261 tcagatgcac attttgaaat acttgcagcg ctttctcccg ttaacagtaa aaaggttatc
     4321 ctggcgctga gggattattt tgttctgggg ctgacgcgca gggaagcctg tgaaaatcac
     4381 gatgtggcac aaggctattt ttcggtgagt ctgcggaaat tactggaaac caaccgcctt
     4441 gtggcggagc tggctgagtt gttgtcggtc ccttctgaat gcagaccata ctggtaaacc
     4501 agtaaggctg tagtgcccga actgcgctct gttgtcccgg gagcatggag gatatgtgcc
     4561 tggtttactt tggtcaggat gatgaatgtc gggagaattc tttttccaga tattcaagat
     4621 ttctgctgct gatatcagcg ggagaagaag cgccgatcag cgtcatggtg actttcatat
     4681 cttcagcata aagtctgagc aggtgctcaa cgcctttttt tcccgcagcg gcaagtgcgt
     4741 aaatatacgc tcttcccagc agcacacctt ttgcccccag agcaagcatc cggaccacat
     4801 ccacaccgga acgaatacct gaatccgcca gtaccgtgat gtcatcaccc actgcatctg
     4861 ctatcgccgg taatgccctg gccgtgggga tggcaccatc aagctgacgt ccgccatgat
     4921 tggagacaat gatgccatcc gcgccaagcc gtacagcatt ttttgcatct tccggaacaa
     4981 ggatcccttt tattatcagt ttacctttcc agccatcacg gatccattcg agatcgttcc
     5041 atgcaataga gggatcaaag tttttgctga taaagcccat atagtcatcc atcgacattt
     5101 tatgcccggt gtacgtttcg atgttaccaa atgacagagg acgaccggca attcccacat
     5161 ccagcgccca gcggggatga agaacggcct gaagatactg acgcagtgtg gcgtggggac
     5221 ctgacatccc tgaatggtta tccctgtacc gggagcccgg cacaggcatg tcgacggtaa
     5281 ataccagggt tttcatacct gcagtccagg cgcgttcaag tgcgttttgc atataaccac
     5341 gatcccgaag gacgtataac tgggaccaca gttgcccgtc agcgtgtccg gcgacctctt
     5401 cgatcgggca gacggagacg gttgagagag tataagggat gttcattgct gaggccgctc
     5461 tggctgcctg cacctctcca cgacgggcgt acatgccagt ggcaccgacg gggcccagag
     5521 caacaggcat ggcccattct gaatcaagga tggtggtgtt cagttcgggg gctccggcgc
     5581 cacaaagcac ctgctgccgg aggctgaccg aagataaaga ggtcatattt tcatgcatgg
     5641 ttttttcagt aacagccccg ccgtctatgt aatcaaaaag aaagcgtggc agtctgtgtt
     5701 ttgcggcgtc cctgtaatct gcaggtgagg aaataatcat ttaaatactc ctgatatttc
     5761 tggtgataac aacagacaga gtgtgttctg taatgctggt gtgaatgttg ttgtaattat
     5821 tattgttctg tttatgttat gtaaaatgaa atgccggaat gacttttatg cgtgcatgga
     5881 atggtgcaga gatgttttaa gtttgcttca gagtgcgcat tccgatgtat tcgatcagtg
     5941 attcgcacga agctcgatca tttattcgca ctccatcccc ttacccacaa gttgaaagta
     6001 agcgtaaacc gacagccgta tgtagcaatt aagccagtac tggtaacgac aggtgcccac
     6061 ctttttaacc agtggacacc tgttatggga caaaaaacat tcttataaaa tgcgttatgg
     6121 tgacgtacag ataaaccgtc atgatgtcaa atgagtgggt cagcacatgc gcccgttgct
     6181 tacaaaaaga gctttaaacc ccggagggga gcaggctaca tatgccctgc caaatctgtt
     6241 cgtcactatg ctaactcaat tttaatatac aggaggtcga taatactttt cctttatgtt
     6301 taccagcctg ccgggagatg gcccagcgca gaagcaattg catacgcagc gctacagaga
     6361 tataacaaca caataaagct ctggagtagc ggatgaataa tgagtgcagg acacgtccag
     6421 ccaggtgtca ggtcaccgtg ctggcgcgcc cctttcagta tcagaatggg cataacagac
     6481 aggaccacgc cactgaagac ccccgcaaaa taaagcgcat ttacaaacga taccatgccg
     6541 ctataagcaa gaataaacgg tggtatgaca acgatcagca gtaacagaaa gcgtcttaga
     6601 ggctggctgt catcgcccgg actgaactgg tcaacgatat tggtcagaaa actgccacca
     6661 agtccccagt aagaagtcag catcgcgcaa agagcaaaca gattcgcaga gaaaaaggcc
     6721 cactcaccca gcgcacgtcc ccaggaaatg gtcgccacat cagaaatatt gtccagcccg
     6781 ttgagtgcta tgacagacat aggcaccaga gccagcagaa tgaaggtaat tcccatcccg
     6841 accataattg ccttcggcaa tttttctggt ttatcagaaa aaccgcgcgc catttccgga
     6901 acaatatatt gtgccgagaa acaaaatgcc acaacgttaa agaccggcac catatacagc
     6961 cagttgccat ccagcaggta acgcatttgt gttgtgtctt tcagcattgt ggcgacgata
     7021 aggatagtga gcatcaccac cataccgata cttataaatt tttccccgcg acctatcgct
     7081 ttcaggccga gataaagcac tcctgcggcg ggaatgaaaa ataacatgct acccagagct
     7141 ggagaaatgc ccagcagtga gtgcagcagc ttaccgcttc cgctcatata tgcaatcaga
     7201 gcgccaacgc tgttcacaca gacggaaagg aacattatca gcgcgccaca gcgtccgaca
     7261 taacgatgtg acaatccgct taactgcaaa tgtttacggg tacgtaaagt cgattcagcc
     7321 acatacagca tggttatggt ggtcaggctc ccgaccagca cgagccagaa taacagcggc
     7381 agaaaaccgg ctttaccgga ggcgtaggcc acagacagta cacctgcgcc aatgttcgtg
     7441 ccgacaatca tcgacacacc ttcaacgaaa ctcagggatt tcgccggcgc agcggtatgt
     7501 ttgcggcatg actgttcagg ggaaaaggtg gcattttcag acataacaga ttccgtatta
     7561 caccggcgac tattgccgcc ggaaaaaggt gatatcagga ataaatcact cacgcctggc
     7621 acaaacgaca cgtgggaaag aaaggcttcc ctgccgttgt ggcaattttc cgtgcatatc
     7681 aggtacgcag aattcgggtc agaatattca gcgctttgct gaactgagca tccgggattg
     7741 ttaaagggta gagaaaacgg ataacgttac cgtagatacc acagttcaac agcagcaggc
     7801 cgttttcccg ggctttgtgc tgtacctgtc gtgcatattc cgccgacggc tcaccgcttt
     7861 gcggattgtt aaactccact gccaccattg aacccagtcc acgcacatcc gcaattgccg
     7921 gacatcctgc ccgggcctga ctgagcacct ctttcagatg ttcgccaagt tgatcggcac
     7981 gctggcagag ctgttcttct tcaatgatat ccagcaccgc atgcgcagca gctactgcca
     8041 gcggattacc ggcataagta cctcccagtc ctcccggagc cggggcatcc atgacttcgg
     8101 cacgccccac aacccccgat aacgggaagc cgccagccag acttttcgcc attgttatca
     8161 gatcggcttt cacagcgtag tgctgcatcg caaatagcct gccagtacgg gcaaaaccgg
     8221 tctgtacttc gtcggcaacc agcaggatcc cgtgtgtatc gcacaactca cgcaatgcct
     8281 gcataaaagc ggccggagcg acattgaacc cgccttctcc ctgaacgggc tccagtacaa
     8341 tcgcggcgac ctgatcagca ggaatatctg ctttaaagat acgatcaaga ctcctgagag
     8401 catcatcaac ggaaatgcca tgggcatcat taggatagac ggcgtggtaa actgagcccg
     8461 ggaacgggcc aaaccctgtt ttgtagggag cgactttgcc cgtgagtgcc atggtcatga
     8521 atgtacgacc atggaaccca ccgccaaaag taataatccc cggacgaccg gtgtatgcgc
     8581 gcgcaatttt caccgcattt tcaactgctt cggcaccggt ggtaaaaaat gccgttttgg
     8641 caggtccatc gacaggggcg acagcattaa tacgttctgc aagcgaaacg taactctcat
     8701 aagggacaat ctgataagcg gtatgggtaa acgactgcaa ctgctgctct accgccgcca
     8761 caatttttgg atgccgatgg ccggtgttca gtacggcaat accggcggca aagtcgataa
     8821 cttcattgcc ttccacatcc cacagcgtgg cgttctccgc tttcccgaca aagtagtcac
     8881 acattacccc cacaccacgg ggagtcgcct gctggcgacg atgattcagt tcagaatttt
     8941 tcattcccga ctctcctgtt aaatatgtca cttaactgca acaaatgaga cgctgtgttg
     9001 tttatttagg caggcgatgg cactataatc gagtaccaat tttgattatc tgagggatcc
     9061 agttgagggc attcataggc gatctgctgc tgttcagaat gggtcaggag caggataaac
     9121 ttctgcacaa acggctctat aatgcgcttc gctatgctat tctcgatggc tctttaccgc
     9181 cgcacagccg tctccctgcc tccagggaac ttgcagttga acttggaatc tcaagaaaca
     9241 cagtattaac aacttatgaa caattacagg cggaaggata tgtggtatca cgtcgtggaa
     9301 gcggaacatt cgtcgcacag acggctcctg ataccttctt aatggcgttc agtaacacgg
     9361 aaaaaagtga agatgccagg ctgagttctg ctgtttctca ccgcggggat tatctgctcc
     9421 ggcatgtcag cgccagtccg tgccagtggg gggcatttat tcccggcgtc ccggatgtca
     9481 gtgcctttcc ccaccccctg ttcagcagga ttcaggcgcg tatcagtcgc cgtcctgagc
     9541 cggagcggct gagctatagc tgtaccggag ggacaccgga attacagcat gcactggttg
     9601 attatttacg gattgcgcgt ggcgtacgct gtcaggctga tcagatactt atcaccgaag
     9661 gtatccatca ggcgatcgat cttgttgcac gcatgctggc agatgccggt gatatggcat
     9721 gggtggaaga gccgtcttac tggggaatac gccatgtact ggcaatgaat gctgtgcaga
     9781 tcacccctgt tgccgttgat gccagtggtc tggtacctcc cgaacgcctg atgccacctc
     9841 ccaggctgat tttcgtcacg ccatcccatc aataccctct gggcgtggtg atgagtcttg
     9901 agcgacggca acgcttgctg gcccttgcca gacagcatgg aagctggatt gtggaagatg
     9961 attatgacag tgaatttcgc ttttccggtc aaccagttcc ggcactacag gggcttattg
    10021 acgatgcacc agtggtgtat attggtactt tcagcaagac agtgtatccc ggactgaggc
    10081 tgggttatct ggtattaccg cgcccgctga aagaggcttt aaaacgtgcc catgctgagc
    10141 tttatcgtgg cgggcataca ctgatccaga tggcactggc tgagtttatc aacggagggc
    10201 attattctgc tcatattcgc cgtatgcggt tactgtacag tcgccgtcgc gcctttcttg
    10261 gtgaactgat tgaacgtcac tgtggaacac aggcactgtc agatttcagc gataatgccg
    10321 ggctgcatct gatattaaat ttaccggacg atgttgatga tgttgcggtt gcggccagcg
    10381 ccaatgcaca aaatatactg gtgcgtccgc tgtcgcttta ctacctgaca gcacagcgta
    10441 aaagaggact gttgatggga tttgcgagtc tgccggaagc gcagatggaa gaggctttcg
    10501 ccacgcttct gacctgtttg cgccatcact gccctcaggt gctggtaaag gtctgaggtg
    10561 gctggtaaca gagggaacaa cactctggtc aggagtattc acatttcact ttacatcgtt
    10621 tctctgagca gacagtagtt aatcatcttc aatgagttca gctatagtgg taataataaa
    10681 gtgatgtctt taacacatca gataaagtgg tcaacattcc ggttttgata accgggagaa
    10741 cacataaaaa tctttctgaa ataattgatg aaagcctgca ttgcaggcgt aaactcaaca
    10801 ttattacgcc atgccaggcc aatactcatt gggaaaactt ctgatttaga taatgcgcgt
    10861 gtttctatac gtttcccttc aagcgaccat gggcgataaa ccatgtcaga aagaatggtt
    10921 atacccatat cattcgcaac catacttcgt acagcttcaa tagaatgcgt atgtaaaatt
    10981 acatctggca tggtactggc cgtattccag taacgttttg ctgtttccca tgcttcatcc
    11041 acatcaagga taataaaagg ctctgagaca atatcattca gggagagaat ttctttttcc
    11101 agcagagggt ggccggatgg taaccatata cggcgctccg accgtaacag tgtttctgat
    11161 acaatatcgg gacatgtcag attatcattg attacaatcc caatatcata ttttccgctg
    11221 agaagtccgg tttcaataat gcttctttga ttttcaaaaa gctccagctt tatatgtgga
    11281 taaattcttg aaaattgttt cagatgaaca ggtaaaaaat atcccattat tgtataagtc
    11341 gccgcaatgg aaactgttcc atgcaaaggc ttctgaacat gcactgtatt tattgcctca
    11401 tcatatgtcc ggataatatt gtaggcattg tataagaaca gtcttccctc atctgttaaa
    11461 tcaacgccag attttgagcg aatcagtaat ttataaccga tgtccctttc aagcccctga
    11521 attgctgttg ttatcgcaga ctgtgaaata ttgagtgatt tggctgcagt tgataacgat
    11581 ccggactccg ctgttgatac gaaatatttt atctgtctga gtgtgatgtt catcaaattt
    11641 tcctgataaa gatatttttg caacagcaat tataaataaa tttaatatat taccatagaa
    11701 aaataataat ttacaataaa gaaacaaatg tgctacatga catactgtta ccgtatttgt
    11761 cagacggagg ttttaatgct tcgcagtatt gatatcagct ctgatatggg ggaaagcttt
    11821 ggagcatgga ctctcggaga tatccgggat attgatatta tgccatacat ctcctcagcg
    11881 aatatagcga caggttttca tgcaggtgat ccggggatta tgcaccgtac aataatcagc
    11941 gcccgtgaca gtggtgtgtc tgtcggtgct catcccggtt atcgtgatct ggctggtttt
    12001 ggccggcgtc atattcactg caccgcagag gaaatcacca atgacatgat ttatcagctg
    12061 ggggccttgc gtgaatttgc cacattacac aatgtgcctg taaaccatgt taagcctcat
    12121 ggcgcgttgt atatgcatct tgctcatgat cgggatgctg caaagcattt tgtctcaacg
    12181 cttcatagtt atgcgcctga attgagaata tattgcatgt atggtagtgc catctggaaa
    12241 gaagcgacgc aaatcggaca tcctgttatt tgtgagtttt atgccgacag ggattatgat
    12301 caaagcggtt ccattgtatt tatacggaga actgaacctc ttaatcctga tgatgtcgca
    12361 aaaaaagtgc tgcgtgcctg tctggaggga aaagttcgta ctgtagaaga tcaggatatt
    12421 gacattcatt tctcttctgt ctgtgtccat agtgatacgc ccggtgcctt atctcttctg
    12481 attgcaataa ataaagcctt acgtgaggca gacataagtg taaattctca aaactgatat
    12541 acatggagga tgttatggct gttacagaaa ttctttcacc tttacctggt gtattttatc
    12601 ggaaaccagc acctgatagt gccccgtttc tggagaatgg tgacactgtt caggagagta
    12661 ccgtcattgg gttgattgaa ataatgaagc agtttaacga aattcatgct gaaacaaccg
    12721 ggactaatat ccagtttaag gtggagaatg gggaggaagt ggaacctggt caggtactgg
    12781 ctaccataga aataatataa ggaactgagc atgagtattc aaaaactgct tattgccaac
    12841 agaggtgaga ttgcattacg aattatacga agtgcaaaga agctgaatat aaaaacggtg
    12901 ctggcatgta gcgaatcaga catcaattct cttccggcaa agagcgccga tgaagttatt
    12961 gtcataggta aatcccgtgc cgatgacagt tatctggacg gaaagaagat aattgatgca
    13021 gcccttcagt ctggcgctca ggccattcat cccggatatg gttttctgtc ggagaatgcg
    13081 tcttttgccc gtgcggtgct cgatgcaggg cttatttttg ttgggccatc tcctcagatt
    13141 attgagcaaa tgggggataa agcaatcgcg agagattgcg cgataaaggc aggtgtcccg
    13201 gttgtaccag gctcaaatgg cgaggttata agccctgagg aggcattatc agtagcatcg
    13261 gatattaccg gatatccttt attgataaag gcctcggccg ggggaggcgg gaaggggata
    13321 cggattgtac attctccgga acagctggtg catgaatata cagtagcgcg tgctgaagca
    13381 aaagcagctt tcgggcacga tggtgtatat ctggaacgtt atataccgga agcccgtcat
    13441 gttgaagttc agatccttgg agacggaaaa aacatcgttc acctgtttga acgagagtgt
    13501 tctctgcaga gaaaaagaca aaaagtgaca gaagaagcgc catcgccttc tgtttcagcg
    13561 tcactacgtg aggccttatg tcagagtgca ctgaatctcg ccagaatgct gaattattca
    13621 agcgcaggaa ctattgagta cctggtcgat gaccggagcg gggaattctt ttttattgaa
    13681 atgaatacgc ggattcaggt tgaacatccg gttacagaaa tggtcacagg cattgatatt
    13741 atcgactgga tgctgagaat tgccgggggc gactctctgt cgtttggaca ggatgagatt
    13801 acgttacagg gagctgcaat tgaaatgagg attaatgcag aaaatcctta caatggtttt
    13861 atgcccagtc ccggaacaat atcggtattg cagatacctg aagggcaggg aataagagtc
    13921 gatactcaca tgtttgaagg gtataagatg cctctctggt atgattcttt actggcaaaa
    13981 cttattattc atactgacag cagagaaaaa acgataagtc ttgcccggga gacccttggt
    14041 aatatgcaaa tagaaggcgt tcatacaact atacctcttc atgaagcatt attatcagct
    14101 gagtttttca gaaatgctca gtttcataat aaaacgcttg aagactggct gaataccggt
    14161 gcatttaaat gaggaacctg tatgactgat ataaatttgc gctatacatt tggtgctgat
    14221 gagcatcttt tcatcgaagt cagtgaggaa atgtctctta aagcattttt tcacagcttg
    14281 tctcttacga gaaaaataga atcattaaat attaatggtg tgctcgatat ttgccttgca
    14341 aatgcatcat gtcagataag atttaatccc gacattatac accctgatga tttgttacaa
    14401 cagatacgaa atgttaatgt gctctccggg aatggagaaa tgacactaca gacacgtata
    14461 attgaaattc ctgtattgta taatgatcca tggactcatg aaaccctgat gcggttccgg
    14521 aataatcatc aaaacccggc attaaccgat cttgagtatg ctgctgaaat aaacggatat
    14581 ccggatgttc agacgtttat tgaggcccat cattcaagtc cctggtttgt ttctatggtt
    14641 ggcttcgttg ccgggctgcc ttttatgtat cagatggttg accggaaaaa gcaaatccag
    14701 gttccaaaat atcttgttcc cagaactgat acaccaaaac attctgttgg tcatggcggg
    14761 tgcttcgggg ctatatatcc agtccggggg gcaggtgggt atcaattgtt cggtataaca
    14821 ccagctccga tttatgacac agaacagaaa ttatcatatt tcaaagactc tatggtgttt
    14881 ttccgggcag gggacatcat taagttcagg gcgatcaata ctgaacaata taatcaatgt
    14941 ataagtgatg tggaatctgg taattttgaa ttacttatca ggcctgtaaa ttttgatttg
    15001 aacgcatttg aagaaaatcc tgaagattat actaaaaatt tactgggaga actgtatggg
    15061 aattaaagta ttgacgcccg gattatccac gtccgttcag gacggaggac gagaaggctt
    15121 ttatcatctc gggatcccgc catccggtgc attagatcgt acagggttag ttaccgccaa
    15181 tttgcttgtc ggtaatcacc cgtttgatgc agcgctggaa tgcacattac tggggcccga
    15241 actcttgttt gagcaggata ctctttttgc agtcagcggt gcccctttta cggtgaccct
    15301 ggataatcag agtgttccgg tgaatacatc cctgttagcc cggaaaggac agatattaaa
    15361 aatcgcatac accacggtgg gtgtacgctg ttaccttgcc atcgcgggag gtattaatgt
    15421 ccctgttatt gccggaagtc gttctactta cctccttggc agtcttggtg gttataaagg
    15481 acgtcggtta caggccggag atgaattacc cgtacaggaa gcaatatgta ataaagggcg
    15541 tataaattac actctgccag atgagttacg tccagttttt actaaagaaa atgaattacg
    15601 tgttgtaccc ggaatgtata tccatcgcct gaaacaggaa tcagttgaaa gtttttttga
    15661 tgatgtatgg aagatcagta aggattcaga cagaacaggt ttcaggtttc agggagggcg
    15721 ctcacttaat tttgttgatc ggcacccgcc atttggtgct ggttcagatc catcaaatat
    15781 cgttgatgcc tgttatcccg tgggatcaat tcaggttccc tctggaagca caccaatagt
    15841 tttgcacagg gatgctgtat caggaggggg atatatgaca gtggggacag tagtgagtac
    15901 tgatctcgat attctggctc aacttgcacc cggctccagt gtgacatttt cacaagtcag
    15961 tatggataaa gctctggaaa tccgtaaaga atttaatgaa agactcaacc ttctcattaa
    16021 ttcattatct tatggctcat aacaggtgcg tctgaaatga aattaccgat gaatcttacc
    16081 atagccctgt tagcagggct gtcatgcctg gcttcatggc cggggattaa tattcctgtg
    16141 tgggctatat ttatt
//
LOCUS       DAMZFT010000113        15881 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.113, whole genome shotgun sequence.
ACCESSION   DAMZFT010000113 DAMZFT010000000
VERSION     DAMZFT010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15881)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15881)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15881
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.113"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..2335
                     /gene="lon"
                     /locus_tag="PN977_003727"
     CDS             <1..2335
                     /gene="lon"
                     /locus_tag="PN977_003727"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001469947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HDI6043726.1"
                     /translation="RIEIPVLPLRDVVVYPHMVIPLFVGREKSIRCLEAAMDHDKKIM
                     LVAQKEASTDEPGVNDLFTVGTVASILQMLKLPDGTVKVLVEGLQRARISALSDNGEH
                     FSAKAEYLESPTIDEREQEVLVRTAISQFEGYIKLNKKIPPEVLTSLNSIDDPARLAD
                     TIAAHMPLKLADKQSVLEMSDVNERLEYLMAMMESEIDLLQVEKRIRNRVKKQMEKSQ
                     REYYLNEQMKAIQKELGEMDDAPDENEALKRKIDAAKMPKEAKEKAEAELQKLKMMSP
                     MSAEATVVRGYIDWMVQVPWNARSKVKKDLRQAQEILDTDHYGLERVKDRILEYLAVQ
                     SRVNKIKGPILCLVGPPGVGKTSLGQSIAKATGRKYVRMALGGVRDEAEIRGHRRTYI
                     GSMPGKLIQKMAKVGVKNPLFLLDEIDKMSSDMRGDPASALLEVLDPEQNVAFSDHYL
                     EVDYDLSDVMFVATSNSMNIPAPLLDRMEVIRLSGYTEDEKLNIAKRHLLPKQIERNA
                     LKKGELTVDDSAIIGIIRYYTREAGVRGLEREISKLCRKAVKQLLLDKSLKHIEINGD
                     NLHDYLGVQRFDYGRADNENRVGQVTGLAWTEVGGDLLTIETACVPGKGKLTYTGSLG
                     EVMQESIQAALTVVRARAEKLGINPDFYEKRDIHVHVPEGATPKDGPSAGIAMCTALV
                     SCLTGNPVRADVAMTGEITLRGQVLPIGGLKEKLLAAHRGGIKTVLIPFENKRDLEEI
                     PDNVIADLDIHPVKRIEEVLTLALQNEPSGMQVVTAK"
     gene            2544..2816
                     /gene="hupB"
                     /locus_tag="PN977_003728"
     CDS             2544..2816
                     /gene="hupB"
                     /locus_tag="PN977_003728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein HU-beta"
                     /protein_id="HDI6043727.1"
                     /translation="MNKSQLIDKIAAGADISKAAAGRALDAIIASVTESLKEGDDVAL
                     VGFGTFAVKERAARTGRNPQTGKEITIAAAKVPSFRAGKALKDAVN"
     gene            3008..4879
                     /gene="ppiD"
                     /locus_tag="PN977_003729"
     CDS             3008..4879
                     /gene="ppiD"
                     /locus_tag="PN977_003729"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HDI6043728.1"
                     /translation="MMDSLRTAANSLVLKIIFGIIIVSFILTGVSGYLIGGGNNYAAK
                     VNDQEISRGQFENAFNSERNRMQQQLGDQYSELAANEGYMKTLRQQVLNRLIDEALLD
                     QYARELKLGISDEQVKQAIFATPAFQVDGKFDNSRYNGILNQMGMTADQYAQALRNQL
                     TTQQLINGVAGTDFMLKGETDELAALVAQQRVVREATIDVNALAAKQPVTEQEIASYY
                     EQNKNNFMTPEQFRVSYIKLDAATMQQPVSDADIQSYYDQHQDQFTQPQRTRYSIIQT
                     KTEDEAKAVLDELNKGGDFAALAKEKSADIISARNGGDMGWLEDATIPDELKNAGLKE
                     KGQLSGVIKSSVGFLIVRLDDIQPAKVKSLDEVRDDVAAKVKHEKALDAYYALQQKVS
                     DAASNDTESLAGAEQAAGVKATQTGWFSKDNLPEELNFKPVADAIFNGGLVGENGAPG
                     INSDIITVDGDRAFVLRISEHKPEAVKPLADVQEQVKALVQHNKAEQQAKVDAEKLLV
                     DLKAGKGAEAMQAAGLKFGEPKTLSRSGRDPISQAAFALPLPAKDKPSYGMATDMQGN
                     VVLLALDEVKQGSMPEDQKKAMVQGITQNNAQIVFEALMSNLRKEAKIKIGDALEQQ"
     gene            5030..5401
                     /locus_tag="PN977_003730"
     CDS             5030..5401
                     /locus_tag="PN977_003730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HDI6043729.1"
                     /translation="MKHGIKALLITLSLACAGMSHSALAAAPAAKPTTVETKAEAPAA
                     QSKAAVPAKASDEEGTRVSINNASAEELARAMNGVGLKKAQAIVSYREEYGPFKTVED
                     LKQVPGMGNSLVERNLAVLTL"
     gene            5507..5905
                     /gene="fadM"
                     /locus_tag="PN977_003731"
     CDS             5507..5905
                     /gene="fadM"
                     /locus_tag="PN977_003731"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain acyl-CoA thioesterase FadM"
                     /protein_id="HDI6043730.1"
                     /translation="MQTQIKVRGYHLDVYQHVNNARYLEFLEEARWDGLENSDSFQWM
                     TAHNIAFVVVNININYRRPAVLSDLLTITSQLQQLNGKSGILSQVITLEPEGQVVADA
                     LITFVCIDLKTQKALALEGELREKLEQMVK"
     gene            complement(5957..6652)
                     /gene="queC"
                     /locus_tag="PN977_003732"
     CDS             complement(5957..6652)
                     /gene="queC"
                     /locus_tag="PN977_003732"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HDI6043731.1"
                     /translation="MKRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEID
                     VARELALKLGARAHKVLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNIL
                     FLTLAAIYAYQVKAEAVITGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPL
                     MWIDKAETWALADYYGKLDLVRNETLTCYNGIKGDGCGHCAACNLRANGLNHYLADKP
                     TVMAAMKQKTGLN"
     gene            complement(6717..8417)
                     /locus_tag="PN977_003733"
     CDS             complement(6717..8417)
                     /locus_tag="PN977_003733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SgrR family transcriptional regulator"
                     /protein_id="HDI6043732.1"
                     /translation="MRLLNRLNQYQRLWQPSAGKPQTVTVSELAERCFCSERHVRTLL
                     RQAQEAGWLEWQAQSGRGKRGQLRFLVTPESLRNAMMEQALETGKQQDVLELAQLAPG
                     ELRTLLQPFMGGQWQNDTPTLRIPYYRPLEPLQPGFLPGRAEQHLAGQIFSGLTRFDN
                     NTQRPIGDLAHHWETSTDGLRWDFYLRSTLHWHNGDAVKASHLHQRLLMLLQLPALDQ
                     LFISVKRIEVTHPQCLTFFLHRPDYWLAHRLASYCSHLAHPQFPLIGTGPFRLTQFTA
                     ELVRLESHDYYHLRHPLLKAVEYWITPPLFEKDLGTSCRHPVQITIGKPEELQRVSQV
                     SSGISLGFCYLTLRKSPRLSLWQARKVISIIHQSGLLQTLEVGENLITASHALLPGWT
                     IPQWQVPDEVKLPKTLTLVYHLPIELHTMAERLQATLAAEGCELTIIFHNAKNWDDTT
                     LLAHADLMMGDRLIGEAPEYTLEQWLRCDPLWPHVFDAPAYAHLQSTLDAVQVMPDEE
                     NRFNALKAVFSQLMADATLTPLFNYHYRISAPPGVNGVRLTPRGWFEFTEAWLPAPSQ
                     "
     gene            8517..9335
                     /gene="cof"
                     /locus_tag="PN977_003734"
     CDS             8517..9335
                     /gene="cof"
                     /locus_tag="PN977_003734"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HMP-PP phosphatase"
                     /protein_id="HDI6043733.1"
                     /translation="MARLAAFDMDGTLLMPDHHLGEKTLSTLARLRERDITLTFATGR
                     HALEMQHILGALSLDAYLITGNGTRVHSLEGELLHRDDLPADVAELVLYQQWDTRASM
                     HIFNDDGWFTGKEIPALLQAFVYSGFRYQIIDVKKMPLGSVTKICFCGDHDDLTRLQI
                     QLYEALGERAHLCFSATDCLEVLPVGCNKGAALTVLTQHLGLSLRDCMAFGDAMNDRE
                     MLGSVGSGFIMGNAMPQLRAELPHLPVIGHCRNQAVSHYLTHWLDYPHLPYSPE"
     gene            9488..9946
                     /gene="decR"
                     /locus_tag="PN977_003735"
     CDS             9488..9946
                     /gene="decR"
                     /locus_tag="PN977_003735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator DecR"
                     /protein_id="HDI6043734.1"
                     /translation="MLDKIDRKLLALLQQDCTLSLQALAEAVNLTTTPCWKRLKRLED
                     DGILIGKVALLDPEKIGLGLTAFVLIKTQHHSSEWYCRFVTVVTEMPEVLGFWRMAGE
                     YDYLMRVQVADMKRYDEFYKRLVNSVPGLSDVTSSFAMEQIKYTTSLPIE"
     gene            9976..11748
                     /locus_tag="PN977_003736"
     CDS             9976..11748
                     /locus_tag="PN977_003736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SmdA family multidrug ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HDI6043735.1"
                     /translation="MRLFAQLSWYFRREWRRYLGAVALLVIIAMLQLVPPKVVGIVVD
                     GVTEQHFTTGQILMWIATMVLIAVVVYLLRYVWRVLLFGASYQLAVELREDYYRQLSR
                     QHPEFYLRHRTGDLMARATNDVDRVVFAAGEGVLTLVDSLVMGCAVLIMMSTQISWQL
                     TLFALLPMPVMAIMIKRNGDALHERFKLAQAAFSSLNDRTQESLTSIRMIKAFGLEDR
                     QSALFAADAEDTGKKNMRVARIDARFDPTIYIAIGMANLLAIGGGSWMVVQGSLTLGQ
                     LTSFMMYLGLMIWPMLALAWMFNIVERGSAAYSRIRTMLAEAPVVIDGSDKVPEGRGE
                     LDVNIRQFTYPQTDHPALENVNFALKPGQMVGICGPTGSGKSTLLSLIQRHFDVSEGD
                     IRFHDIPLTKLQLDSWRSRLAVVSQTPFLFSDTVANNIALGCPNATQQEIEHVARLAS
                     VHDDILRLPQGYDTEVGERGVMLSGGQKQRISIARALLVNAEILILDDALSAVDGRTE
                     HQILHNLRQWGQGRTVIISAHRLSALTEASEIIVMQHGHIAQRGNHDVLAQQSGWYRD
                     MYRYQQLEAALDDAPENREEAVDA"
     gene            11741..13522
                     /locus_tag="PN977_003737"
     CDS             11741..13522
                     /locus_tag="PN977_003737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SmdB family multidrug efflux ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HDI6043736.1"
                     /translation="MRSFSQLWPTLKRLLAYGSPWRKPLGIAVLMMWVAAAAEVSGPL
                     LISYFIDNMVAKNNLPLKVVAGLAAAYVGLQLFAAGLHYAQSLLFNRAAVGVVQQLRT
                     DVMDAALRQPLSEFDTQPVGQVISRVTNDTEVIRDLYVTVVATVLRSAALVGAMLVAM
                     FSLDWRMALVAIMIFPVVLVVMVIYQRYSTPIVRRVRAYLADINDGFNEIINGMSVIQ
                     QFRQQARFGERMGEASRSHYMARMQTLRLDGFLLRPLLSLFSSLILCGLLMLFGFSVS
                     GTIEVGVLYAFISYLGRLNEPLIELTTQQAMLQQAVVAGERVFELMDGPRQQYGNDDR
                     PLQSGTIEVDNVSFAYRDDNMVLKNINLSVPSRNFVALVGHTGSGKSTLASLLMGYYP
                     LTEGEIRLDGRPLSSLSHSALRQGVAMVQQDPVVLADTFLANVTLGRDISEERVWQAL
                     ETVQLTELARSMNDGIYTPLGEQGNNLSVGQKQLLALARVLVETPQILILDEATASID
                     SGTEQAIQHALAAVREHTTLVVIAHRLSTIVDADTILVLHRGQAVEQGTHQQLLAAQG
                     RYWQMYQLQLAGEELAASVREEESLSA"
     gene            13703..14041
                     /gene="glnK"
                     /locus_tag="PN977_003738"
     CDS             13703..14041
                     /gene="glnK"
                     /locus_tag="PN977_003738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HDI6043737.1"
                     /translation="MKLVTVIIKPFKLEDVREALSSIGIQGLTVTEVKGFGRQKGHAE
                     LYRGAEYSVNFLPKVKIDVAIADDQLDEVIDIVSKAAYTGKIGDGKIFVAELQRVIRI
                     RTGEADEAAL"
     gene            14071..15357
                     /gene="amtB"
                     /locus_tag="PN977_003739"
     CDS             14071..15357
                     /gene="amtB"
                     /locus_tag="PN977_003739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter AmtB"
                     /protein_id="HDI6043738.1"
                     /translation="MKIATIKTGLASLAMLPGLVMAAPAVADKADNAFMMICTALVLF
                     MTIPGIALFYGGLIRGKNVLSMLTQVTVTFALVCILWVVYGYSLAFGEGNNFFGNINW
                     LMLKNIELTAVMGSIYQYIHVAFQGSFACITVGLIVGALAERIRFSAVLIFVVVWLTL
                     SYIPIAHMVWGGGLLASHGALDFAGGTVVHINAAIAGLVGAYLIGKRVGFGKEAFKPH
                     NLPMVFTGTAILYIGWFGFNAGSAGTANEIAALAFVNTVVATAAAILGWIFGEWALRG
                     KPSLLGACSGAIAGLVGVTPACGYIGVGGALIIGVVAGLAGLWGVTMLKRLLRVDDPC
                     DVFGVHGVCGIVGCIMTGIFAASSLGGVGFAEGVTMGHQLLVQLESIAITIVWSGVVA
                     FIGYKLADLTVGLRVPEEQEREGLDVNSHGENAYNA"
     gene            complement(15406..>15881)
                     /locus_tag="PN977_003740"
     CDS             complement(15406..>15881)
                     /locus_tag="PN977_003740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HDI6043739.1"
                     /translation="LPSETQIAQSLAHLLPPVLKDKFICDRPLEVRPVEFHNPLKGHV
                     AEPHRQVWIRANGSVPDDLRVHQYLLGYASDLNFLPVALQPHGIGFLEPGIQIATIDH
                     SMWFHRPFNLNEWLLYSVESTSASSARGFVRGEFYTQDGVLVASTVQEGVMRNHN"
BASE COUNT         3792 a         3838 c         4461 g         3790 t
ORIGIN      
        1 acgcattgaa atccccgtat tgccgctgcg cgatgtggtg gtttatccgc acatggtcat
       61 ccccttattt gtcgggcggg aaaaatctat ccgttgtctg gaagcggcga tggaccatga
      121 taaaaaaatt atgctggtcg cgcagaaaga agcttcaacg gatgagccgg gtgtaaacga
      181 tcttttcacc gtcgggaccg tggcctctat attgcagatg ctgaaactgc ctgacggcac
      241 cgtcaaagtg ctggtcgagg ggttacagcg cgcgcgtatt tctgcgctct ctgacaatgg
      301 cgaacacttt tctgcgaagg cggagtatct ggagtcgccg accattgatg agcgggaaca
      361 ggaagtgctg gtgcgtactg caatcagcca gttcgaaggc tacatcaagc tgaacaaaaa
      421 aattccacca gaagtgctga cgtcgctgaa tagcatcgac gatccggcgc gtctggcgga
      481 taccattgct gcacatatgc cgctgaaact ggctgacaaa cagtccgttc tggagatgtc
      541 cgacgttaac gaacgtctgg aatatctgat ggcaatgatg gaatcggaaa tcgatctgct
      601 gcaggttgag aaacgcattc gcaaccgtgt taaaaagcag atggagaaat cccagcgtga
      661 gtactatctg aacgagcaaa tgaaagctat tcagaaagaa ctcggtgaaa tggacgacgc
      721 gccggacgaa aacgaagccc tgaagcgcaa aatcgacgcg gcgaaaatgc cgaaagaggc
      781 aaaagagaaa gcggaagcag agttgcagaa gctgaaaatg atgtctccga tgtcggcaga
      841 agcgaccgta gtgcgtggtt atatcgactg gatggtacag gtaccgtgga atgcgcgtag
      901 caaggtcaaa aaagacctgc gtcaggcgca ggaaatcctt gataccgacc attatggtct
      961 ggagcgcgtg aaagatcgca tccttgagta tcttgcggtt caaagccgtg tcaacaaaat
     1021 caagggaccg atcctctgcc tggtagggcc accgggggta ggtaaaacgt ccctggggca
     1081 gtccatcgcc aaagccaccg ggcgtaaata tgtccgtatg gcgctgggcg gcgtgcgtga
     1141 tgaagcggaa atccgtggtc accgccgtac ttacatcggt tctatgccgg gtaaactgat
     1201 ccagaaaatg gcgaaagtgg gcgtgaaaaa cccgctgttc ctgctcgatg agatcgacaa
     1261 aatgtcttct gacatgcgag gcgatccggc ttccgcactg cttgaagtgc tggatccaga
     1321 gcagaacgta gcgttcagcg accactacct ggaagtggat tacgacctta gcgacgtgat
     1381 gtttgtcgcg acgtcgaact ccatgaacat tccggcaccg ctgctggatc gtatggaagt
     1441 gattcgcctc tccggttata ccgaagatga aaaactgaac atcgccaaac gtcacctgct
     1501 gccgaagcag attgaacgta atgcactgaa aaaaggtgag ctgaccgtcg acgatagcgc
     1561 cattatcggc attattcgtt actacacccg tgaggcgggc gtgcgtggtc tggagcgtga
     1621 aatctccaaa ctgtgtcgca aagcggttaa gcagttactg ctcgataagt cattaaaaca
     1681 tatcgaaatt aacggcgata acctgcatga ctatctcggt gttcagcgtt tcgactatgg
     1741 tcgcgcggat aacgaaaacc gtgtcggtca ggtaactggt ctggcgtgga cggaagtggg
     1801 cggtgacttg ctgaccattg aaaccgcgtg cgttccgggt aaaggcaaac tgacctatac
     1861 cggatcgctt ggcgaagtga tgcaggagtc cattcaggcg gcgttaacgg tggttcgtgc
     1921 gcgtgcggaa aaactgggga tcaaccctga tttttatgaa aaacgcgaca tccacgtcca
     1981 cgtaccggaa ggtgcgacgc cgaaagatgg tccgagtgcc ggtattgcta tgtgcaccgc
     2041 gctggtttct tgcctgaccg gtaacccggt tcgtgccgat gtggcaatga ccggtgagat
     2101 cactctgcgt ggtcaggtac tgcctatcgg tggtttgaaa gaaaaactac tggcagcgca
     2161 tcgcggcggg attaaaacag tgttaattcc gttcgaaaat aaacgcgatc tggaagagat
     2221 tcctgacaac gtaattgccg atctggatat tcatcctgtg aaacgcattg aggaagttct
     2281 gactctggcg ctgcaaaatg aaccgtctgg catgcaggtt gtgactgcaa aatagtgacc
     2341 tcgcgcaaaa tgcactaata aaaacagggc tggcaggcta attcggactt gccagccttt
     2401 ttttgtctcg ctaagttaga tggcggattg ggcttgccct tattaagggg tgttgtaagg
     2461 ggatggctgg cctgatataa ctgctgcgcg ttcgtacctt gaaggattca agtgcgatat
     2521 aaattataaa gaggaagaga agagtgaata aatctcaatt gatcgacaag attgctgcag
     2581 gggctgatat ctctaaagct gcggctggcc gtgcgttaga tgctattatt gcttccgtaa
     2641 ctgaatctct gaaagaaggg gatgatgtag cactggtagg ttttggtact tttgccgtta
     2701 aagagcgtgc tgcccgtact ggccgcaacc cgcagaccgg taaagagatc accatcgctg
     2761 ccgctaaagt accgagcttc cgtgcaggta aagcactgaa agacgcggta aactaagcgt
     2821 tgtccccagt ggggatgtga cgaagttcaa gggcgcatct actgatgtgc cttttttatt
     2881 tgtaatcggt gactttctgc gtcttgtggg ctgacaattg cccccgtttc ttgtcacaat
     2941 aggcctttgc gcgcatcgat acgttgcgtg aggtacacag tcatctacag cggagtgttg
     3001 ttacaccatg atggacagct tacgcacggc tgcaaacagt ctcgtgctca agattatttt
     3061 cggtatcatt atcgtgtcgt tcatattgac cggcgtgagt ggttacctga ttggcggagg
     3121 caataactac gccgcaaaag tgaatgacca ggaaatcagc cgtgggcagt tcgagaatgc
     3181 cttcaacagc gagcgtaatc gcatgcagca acagctgggc gatcaatact ctgagcttgc
     3241 agcgaacgaa ggctatatga aaaccctgcg tcaacaggtg ctgaatcgtc tgatcgacga
     3301 ggcgcttctg gatcagtacg ctcgtgagct gaaactgggt atcagtgatg agcaggttaa
     3361 acaggcgatt ttcgcgaccc cagccttcca ggttgacggc aaatttgata acagccgcta
     3421 taacggtatc ctcaaccaga tggggatgac cgccgatcag tacgcccagg cgctacgtaa
     3481 ccagctcact acccaacagc tgattaacgg cgttgccggt accgatttta tgctgaaagg
     3541 tgaaaccgac gagctggcgg cactggtagc tcaacaacgc gtggtacgtg aagcgactat
     3601 cgatgttaac gcgctggcgg cgaagcagcc tgtgaccgaa caggaaattg ccagctacta
     3661 cgaacaaaac aaaaacaatt tcatgacgcc ggaacaattc cgcgtgagtt acatcaagct
     3721 ggatgccgca acgatgcagc aaccggttag cgatgcggat atccagagct actacgatca
     3781 gcatcaggat caattcaccc agccgcagcg tacccgctac agcatcatcc agaccaaaac
     3841 tgaagatgaa gcgaaagcgg tacttgatga gctgaataaa ggcggtgatt ttgctgcgtt
     3901 agccaaagaa aaatctgccg atattatctc tgcccgtaac ggcggcgata tgggttggtt
     3961 agaagatgcc actatcccgg acgaactgaa aaatgctggt ctgaaagaaa aaggccaact
     4021 ctctggtgtc atcaaatctt cggtcggttt cctgattgta cgtctggacg acattcagcc
     4081 agcgaaagtg aagtcgttag acgaagtgcg tgacgatgtc gcggcgaaag tgaaacacga
     4141 aaaagccctc gatgcgtact acgcactgca gcagaaagtg agcgatgcag caagcaatga
     4201 taccgagtct ctggccggtg cagagcaagc tgccggcgtt aaagccactc agacgggttg
     4261 gttcagcaaa gataacctgc cggaagagtt gaacttcaag ccggttgctg acgctatctt
     4321 caacggcggt ctggtaggtg aaaacggtgc gccgggcatc aactctgaca tcatcaccgt
     4381 agacggcgac cgcgcattcg tgctgcgcat cagcgagcac aaaccggaag cggtgaaacc
     4441 gttggcagat gttcaggaac aagttaaggc actggttcag cacaacaaag ctgaacaaca
     4501 ggcgaaagtg gatgctgaga aactgctggt tgatttgaaa gccggcaaag gtgcggaagc
     4561 tatgcaggct gccggtctga aatttggcga gccgaaaacc ttaagccgtt ccggtcgtga
     4621 cccgattagc caggcggcgt ttgcactgcc actgccagcg aaagacaaac cgagctacgg
     4681 tatggcgacc gatatgcaag gtaatgtggt tctgctggcg ctggacgaag tgaaacaagg
     4741 ttcaatgccg gaagatcaga aaaaagcgat ggtgcagggt atcacccaga acaacgcaca
     4801 aatcgtcttt gaagcgctga tgagtaacct gcgtaaagag gcgaaaatca aaattggcga
     4861 tgcgctggaa cagcaataat cctgaagccg cctcgcaaaa aaatgctttg cagctgtaac
     4921 aagaaaaggt cgctttcgcg gccttttcca tttctgaaca ttgccatttg tttactgttt
     4981 tcactgccgt taaggtgatt ccactgttaa caaacaagga gaaaacagta tgaaacacgg
     5041 aattaaagca ctgctcatta ccctgtccct ggcctgtgcc ggaatgtctc atagtgctct
     5101 ggcggcagct cctgcggcga aaccgacaac ggtagaaacc aaagcggagg ctcctgcagc
     5161 acaaagtaaa gcagcagttc cggcgaaagc cagtgacgaa gaaggcaccc gggtcagcat
     5221 taataatgcc agcgcggaag agttagcccg cgcgatgaat ggcgttggcc tgaagaaagc
     5281 gcaggcgatt gtcagttatc gcgaagagta cggtccgttt aaaacggtgg aggatctcaa
     5341 gcaggtgccg gggatgggca attcgctggt ggaacgtaat ctggcggtat taaccctgta
     5401 attaatttgc atagtggcaa ttttttgcca gactgaagag gtcataccag ttatgacctc
     5461 tgtacttata acagttttta taaaaacaac gtaaggttat tgcgctatgc aaacacaaat
     5521 caaagttcgt ggatatcatc tcgacgttta ccagcacgta aacaacgccc gctaccttga
     5581 atttctcgaa gaagcccgct gggatgggtt ggaaaatagc gacagttttc agtggatgac
     5641 ggcccataac atcgccttcg tcgtggtcaa tatcaatatt aactatcgtc gcccggcggt
     5701 attaagcgac ctgttaacta ttaccagtca gttgcagcaa ttaaacggta aaagtggcat
     5761 cttaagccag gtcattacac tggagccgga agggcaggta gtagcggatg cgcttattac
     5821 gtttgtttgt attgatctta aaacgcagaa agcattagct ctggaagggg aattgcgcga
     5881 aaagctggag cagatggtta agtaaacgtt ttgtggtgcc ggatgctcaa gccgcatccg
     5941 gcgacacccg gaataattaa ttcaacccgg ttttctgctt catcgctgcc atcaccgtcg
     6001 gtttatcggc cagataatga ttcaaaccgt tggcgcgtaa attacatgcc gcacaatgac
     6061 cgcaaccgtc gcctttaatg ccgttatagc aggtcagcgt ttcgttacgg actaaatcca
     6121 gcttgccgta ataatctgcc agcgcccagg tttccgcttt atcaatccac atcagcggcg
     6181 tttcaaaacg aatatctttc gccatgccta aactgacggc atggtttagt gctttcacaa
     6241 actcatcgcg gcaatccggg tagccggaga aatccgtttc gcagacgccg gtaatcaccg
     6301 cttctgcttt tacctgatac gcatatattg ccgccagtgt caggaacaaa atattacgcc
     6361 cagggacaaa cgtattcggg atgccatcag cttcaggttc ataatcaggc actggaatgc
     6421 tgtcacgcgt caggctgctg accgccagct cgttgagcag cgttacatca agaaccttat
     6481 gcgcgcgtgc ccccagtttc agcgccagtt cgcgtgccac gtcgatttct gcgcgatgtc
     6541 gctgaccgta atcgaacgtc acgcaatgga cttcatcata ttgttgtaat gcctgcacca
     6601 gacaggtggt ggaatcctgg cctccactga acacaacgac agcacgtttc atagataatc
     6661 ctgcttgaac aataaaagcg ttatggtaac gcctgcgatt aacccggacc agctattcat
     6721 tgcgacggcg cgggaagcca ggcttcggta aattcaaacc agccgcgcgg tgtcagtcgc
     6781 acaccgttca cgccgggagg ggcactaatg cgatagtgat agttgaatag cggcgtcagc
     6841 gtcgcgtctg ccattaactg gctaaaaacc gctttcaggg cattaaatcg gttttcctca
     6901 tcaggcatta cttgcaccgc gtccagtgtc gattgcaaat gtgcatatgc tggagcgtcg
     6961 aaaacatgtg gccacagtgg atcgcaacgc agccattgct ccagggtata ttccggtgct
     7021 tcgccaatta atctgtcgcc catcatgagg tctgcgtgtg ccagtagggt cgtgtcgtcc
     7081 cagttttttg cgttatgaaa aataattgtg agttcacagc cttccgctgc cagtgtcgcc
     7141 tgtagacgtt ctgccatggt atgaagttct atcggtaggt gataaaccag cgtcaaggtt
     7201 ttcggtagtt tgacctcatc cggtacttgc cattgcggaa tagtccagcc tggcagtaat
     7261 gcatgactgg cggtgatcag gttttctccg acttctaacg tttgtaataa accggattga
     7321 tgaataatgg agatcacttt tcgcgcctgc cagagggaca gtcggggact tttgcgcaac
     7381 gttaaatagc aaaaacctaa actgatgcca ctactgacct ggctgacccg ttgcagctcc
     7441 tccggtttgc cgatggtgat ttgcacggga tgccgacaac tggttcccaa atctttttcg
     7501 aaaagcggcg gagttatcca gtactcaacc gctttaagca gcggatgacg taaatggtaa
     7561 taatcatggc tttccaggcg caccagttcc gctgtgaatt gtgttaagcg aaaaggaccc
     7621 gtgccgatca gggggaattg cggatgcgcc agatggctgc aatagctcgc cagccggtgc
     7681 gcaagccagt aatcggggcg atgtaaaaag aaggtcagac actgcggatg ggtgacttca
     7741 atacgcttca cgctaataaa taattgatcc agtgctggca gttgtaacag catcaataac
     7801 cgctggtgta agtgggaggc ttttactgca tcgccgttat gccagtgtaa ggttgagcga
     7861 agataaaaat cccagcgtaa cccgtcagta gaggtttccc agtgatgcgc taaatcgcca
     7921 atcgggcgct gggtattatt atcgaagcgg gtcaggccgg aaaatatctg cccggccaga
     7981 tgctgctcgg cacggccggg caaaaagccc ggttgtagcg gttcgagcgg gcgatagtag
     8041 ggaatacgca acgtgggcgt atcgttttgc cattgtccgc ccataaacgg ctgtaacaga
     8101 gtgcgcagct cacctggggc cagttgcgcc agctccagca catcttgctg ctttccggtt
     8161 tccagcgcct gttccatcat cgcattgcgt agcgattctg gcgtgaccag aaagcgtaat
     8221 tgtccgcgct ttccgcgtcc tgactgcgcc tgccactcca gccatcccgc ctcctgtgcc
     8281 tgacgcaaca gcgtacgaac atggcgttcg ctgcaaaaac agcgttcggc cagttcgctg
     8341 acggtgacgg tttgcggctt tccggcggaa ggttgccaca gacgttgata ctggttaaga
     8401 cggttgagca gtcgcatata aacccggaac aatattattt aactattcac tattacttcc
     8461 gtatatatca ggtgatactc aatcacaatt aaccgtgtca cagagtggag aaagaaatgg
     8521 ctcgtctggc agcatttgat atggatggca ctttattgat gcctgaccat catttaggtg
     8581 agaaaaccct ctctactttg gcgcggctgc gtgaacgcga cattaccctc acttttgcca
     8641 cggggcgtca tgcgctggag atgcagcata ttctcggggc gctatcgctg gatgcatatt
     8701 tgattactgg caacggaacg cgcgtgcatt ctctggaagg tgaactttta catcgtgatg
     8761 atttacctgc ggatgtcgcg gagctggtgc tgtatcagca atgggatacc cgagccagca
     8821 tgcatatctt caatgacgac ggttggttta ccgggaaaga gatccctgcg ttgttgcagg
     8881 catttgtcta tagcggtttt cgttatcaga taatcgatgt caaaaaaatg ccactcggca
     8941 gcgtcaccaa gatctgcttc tgtggcgatc acgacgatct tacacgcttg cagatccagc
     9001 tatacgaagc attaggcgag cgtgcacatt tatgtttttc cgccacggat tgcctcgaag
     9061 tgctgccggt gggctgcaat aaaggcgctg cattgacggt gctgacccaa catttaggtt
     9121 tatcgttacg cgattgcatg gcctttggtg atgcgatgaa cgatcgcgaa atgttaggca
     9181 gtgtcggtag cggatttatt atgggcaatg cgatgccgca actgcgcgcg gagctcccgc
     9241 atttaccggt gattggacat tgccgaaatc aggctgtctc tcactatttg acgcactggc
     9301 tggactatcc acatctacct tattcccccg aataacgaga tcccttccag caccgggcaa
     9361 ttgcccggtt ttttttgcgt tgaatttgtc attttgtgcc gtggtgctta aaccgcacag
     9421 aataaattgt cgcgatttca cctttaaaat agaattaaaa gagaaaaaaa ttctctgtgg
     9481 aagggctatg ttagataaaa ttgaccgtaa gctgctggcc ttgctacagc aggattgcac
     9541 cctctctttg caggcactgg ctgaagccgt taatctgaca accaccccct gctggaagcg
     9601 tctgaaacgg ctggaggacg acggtatcct tatcggcaaa gtcgccctgc tggatccgga
     9661 aaaaataggc ctcggcctga ccgcctttgt gctgataaaa acgcaacatc acagcagcga
     9721 atggtattgc cgctttgtca cggtggttac cgaaatgcca gaagtgctgg ggttctggcg
     9781 catggccggt gaatacgatt atctgatgcg cgtccaggtt gccgacatga aacgctacga
     9841 cgagttttat aagcgtctgg taaacagcgt gccggggctg tcggacgtca cttccagctt
     9901 cgcgatggaa cagattaaat acaccacttc tttacccatc gaataaatat ccagaatcag
     9961 gtcaggacac aacgcgtgcg attatttgct caattaagct ggtatttccg tcgggaatgg
    10021 cgtcgctatc tcggggctgt cgccttgctt gtcattatcg cgatgctgca actggttccg
    10081 ccaaaagtgg ttggtattgt tgtcgatggc gtgacagaac aacactttac taccgggcag
    10141 atcctgatgt ggatcgccac catggtgctg attgccgttg tggtttatct cctgcgttac
    10201 gtctggcgcg tattgctgtt tggtgcgtct tatcaactgg ctgttgaact gcgtgaagat
    10261 tattaccgcc agctaagccg gcagcatcct gagttttacc tgcgtcatcg cacgggtgac
    10321 ctcatggctc gtgcgaccaa tgacgtcgat cgcgtcgtgt ttgccgccgg agaaggggtg
    10381 ctgacgctgg tggattcact ggtaatgggc tgcgctgtgt tgattatgat gtctacgcaa
    10441 attagctggc agttgacctt atttgccctg ttgccgatgc cagtgatggc gatcatgatc
    10501 aagcgcaacg gcgatgcctt gcatgaacgc tttaagctgg cacaggcggc gttttccagt
    10561 cttaatgacc gcacccagga aagcctcacc agtatccgca tgatcaaagc ttttggtctg
    10621 gaagatcgtc agtcggcgtt atttgccgcg gatgccgaag ataccggcaa aaaaaacatg
    10681 cgggtggcgc gtattgatgc tcgtttcgac ccgaccatct atatcgcgat tggtatggcg
    10741 aacttgctgg cgattggcgg cggtagttgg atggtggtac agggcagttt aacgctgggc
    10801 cagctcacca gttttatgat gtatttaggt ctgatgattt ggccaatgct ggcgctggca
    10861 tggatgttta acattgtgga acgtggcagc gcggcgtata gccgaattcg taccatgctg
    10921 gccgaagcgc cagtcgttat tgatggtagt gataaggtgc cagaaggtcg tggtgaactg
    10981 gatgtgaata ttcgccagtt tacgtatccg cagactgacc atcctgcgct ggaaaacgtc
    11041 aatttcgccc tgaaacccgg tcagatggta ggtatttgcg ggccgactgg ttccggcaaa
    11101 agtaccctgt tgtcgctcat tcagcgtcat ttcgacgtca gcgaggggga tatccgcttt
    11161 catgatattc ctctgacgaa gttacaactc gatagctggc gtagccgcct ggcggtagtt
    11221 agccagacac cgttcctttt ttccgacacc gtagcgaaca acatcgcgtt gggttgcccg
    11281 aatgccaccc agcaagagat tgagcatgtc gcgcggttag ccagcgtgca tgacgatatt
    11341 ttgcgtttac cgcaaggtta cgatacagaa gtgggcgagc gcggcgtgat gctttccggt
    11401 gggcaaaaac agcgtatctc cattgctcgt gcgttattag tcaacgcgga aatcctcatc
    11461 cttgatgatg cgctttcggc agtagacgga cgcacagagc accagatcct gcataacctg
    11521 cgccagtggg ggcagggaag gacggtaatc atcagtgccc atcgcctttc ggcactgacg
    11581 gaagccagtg aaattattgt gatgcagcac ggacatatcg cccagcgtgg caatcatgat
    11641 gtgctggcac aacaaagcgg ctggtatcgc gatatgtatc gctatcaaca actggaggcg
    11701 gcgctcgacg acgctccgga aaatcgcgag gaggccgtcg atgcgtagtt ttagccaact
    11761 gtggccgact ctcaagcgtc tgttagcgta cggttcgccg tggcgtaaac cgttggggat
    11821 tgcggtcctg atgatgtggg ttgcggctgc ggcagaagtc agtgggccgc tgcttatcag
    11881 ctattttatc gacaatatgg tagcgaaaaa taacctgccg ttgaaagtgg ttgcagggct
    11941 ggctgcggcg tatgttgggc tgcaactgtt tgccgccggg ctacattacg cgcagtcgct
    12001 gctgtttaat cgggcggcag taggcgtagt gcaacagttg cgtaccgacg tgatggatgc
    12061 tgcgttacgc cagccattaa gcgagtttga tacccaaccc gtcgggcagg tgatttcccg
    12121 cgtcaccaat gacactgaag tgatccgcga tctctacgtt accgtggtgg caactgtcct
    12181 gcgtagcgcc gcactggtgg gcgcgatgct ggtggcgatg ttcagcctcg actggcgaat
    12241 ggcgctggtg gcgataatga ttttcccggt ggtgctggtg gtaatggtga tataccagcg
    12301 ttacagcacg ccgattgtcc gtcgtgtgcg cgcctatctg gcggatatca acgacggctt
    12361 taacgaaatc atcaatggca tgagcgttat ccagcagttc cgtcagcagg cgcgatttgg
    12421 cgaacgtatg ggtgaggcca gtcgttcaca ctatatggcg aggatgcaaa ccctgcgcct
    12481 cgacggtttt ctgctgcgtc cgctgctgag tctgttttca tcgctcattc tttgtggatt
    12541 gttgatgctg tttggcttct ctgtcagcgg caccattgaa gtgggcgtac tgtatgcgtt
    12601 tatcagctat cttgggcggc ttaacgaacc cttaatcgaa ctgaccacgc aacaggcgat
    12661 gctgcaacag gctgttgttg ctggtgagcg cgtgtttgag ctgatggacg gaccgcgcca
    12721 gcaatatggc aatgatgatc gcccgttgca gagcggcacc attgaagtcg ataacgtgtc
    12781 atttgcttat cgcgatgaca atatggtgct aaagaacatt aatctctctg tgccttcgcg
    12841 caattttgtg gcgctggtcg ggcataccgg cagtggtaaa agtaccctcg ccagtttatt
    12901 gatgggctat tacccgctaa cggaaggtga gattcgcctt gatggtcgtc cattaagttc
    12961 gttaagtcac agcgcgctgc gccagggcgt ggcaatggtg cagcaagatc cggtggtgct
    13021 ggcggatacc ttcctcgcca acgtgacgct gggacgtgat atctccgaag aacgcgtctg
    13081 gcaggcgctg gaaaccgtgc aactgacgga gctggcgcgt agcatgaacg acggtattta
    13141 cacgccgctt ggcgagcagg ggaataatct ctcagtcggg caaaagcaac tgctggcact
    13201 ggcacgcgtg ttggtggaga cgccgcaaat cctgatcctt gatgaggcaa cagccagcat
    13261 tgactccggt actgaacagg caattcaaca tgctctggcg gcggtgcgtg agcataccac
    13321 gctggtggtg attgctcacc gcttatcaac tattgttgat gccgacacta ttctggtgct
    13381 tcatcgtggg caagccgtgg agcagggcac tcaccagcaa ctgttggcgg cccagggacg
    13441 ctactggcag atgtatcaac tgcaacttgc gggcgaagag ctggcagcca gcgtgcgtga
    13501 agaggaatca ttgagcgcct gaatagcgca atatttcatc gttggtgcaa taatgtaacg
    13561 cactgtgcac tgtcatagtg cgttttcatt ttcaatcttc ttaacttcct gctctctttc
    13621 tcgtttttca tttctggcac accgcttgca ataccttctt cgtgtagcag aaccattacc
    13681 gaattctgac cggaggggat ctatgaagct ggtgaccgtg ataatcaaac cattcaagct
    13741 ggaagacgtt cgtgaagcgt tatcttccat tggtattcag ggcctgaccg tcaccgaagt
    13801 gaaaggtttc gggcgtcaga aagggcatgc cgagctgtac cggggggcgg aatacagcgt
    13861 caacttcctg ccaaaagtaa aaattgatgt ggcgattgct gatgaccaac tcgatgaagt
    13921 gattgatatc gtcagtaagg cggcttacac cggaaaaatt ggcgacggca aaatcttcgt
    13981 cgctgaattg caacgcgtca ttcgtattcg taccggcgaa gccgacgaag cggcgctgta
    14041 atctctggca cacagcaaca ggaacgaaaa atgaagatag cgacgataaa aactgggctt
    14101 gcttcactgg cgatgctgcc gggactggta atggctgcac ctgcggtggc cgataaagcc
    14161 gacaatgcgt ttatgatgat ttgtactgcg ctggtgctgt ttatgactat tccggggatt
    14221 gccctgtttt acggtgggtt gattcgcggc aaaaacgtgc tgtcgatgct gacgcaggtg
    14281 acggtgacgt ttgcactggt ctgtattctc tgggtggttt acggttactc gctggcgttt
    14341 ggtgagggaa acaacttctt cggcaacatt aactggttga tgctgaaaaa catcgaactg
    14401 acggcggtga tgggcagcat ttatcagtat atccacgtag cgtttcaggg atcgtttgcc
    14461 tgcattaccg taggcttgat agttggagca ctggcggaac gaatccgctt ctcagctgtg
    14521 ttgattttcg tggtggtatg gctgacgctc tcctacattc cgattgcgca tatggtctgg
    14581 ggcggcggtt tgctggcttc tcacggtgca ctggatttcg cgggtggcac ggtggtgcac
    14641 attaacgccg caatcgccgg tctggtgggc gcgtatctga taggcaaacg cgtgggcttc
    14701 ggcaaagagg cgtttaaacc gcacaacctg ccgatggtct tcaccgggac tgccattctc
    14761 tatatcggtt ggtttggctt taacgccggg tcagcgggca cggcgaatga aatcgcagca
    14821 ctggcatttg tgaatactgt ggtcgcaacg gcggcagcaa ttcttggctg gatcttcggt
    14881 gaatgggcgc tgcgcggtaa gccttcactg ctgggagcgt gttctggcgc gattgccggt
    14941 ctggtcggcg tgacgccagc ctgtggctac attggggttg gcggcgcgtt gattatcggc
    15001 gtggtagctg gtctggcggg cttgtggggc gttaccatgc tcaaacgttt gctgcgggtg
    15061 gatgatccct gcgatgtttt cggtgtgcac ggcgtttgtg gcattgtcgg ctgtatcatg
    15121 accgggattt ttgccgccag ctcgctgggc ggcgtgggct tcgctgaagg tgtgacgatg
    15181 ggccatcagt tgctggtaca gctggaaagc atcgccatta cgatcgtctg gtccggtgtg
    15241 gtggcgttta ttggctacaa actggcggat ctgacggttg gtctgcgtgt accggaagaa
    15301 caggagcgag aagggctgga cgtcaacagc cacggcgaga atgcctataa cgcgtaacaa
    15361 gcactgcaaa aaacagccgg acggttttca cctccggcta tttttttaat tgtgattacg
    15421 cattacccct tcctgaacgg tcgaggcaac cagcacgccg tcctgagtat aaaactcacc
    15481 gcgcacaaag ccgcgtgcgc tggacgccga ggtgctctcc acgctataca gcagccattc
    15541 attcaaatta aacgggcgat ggaaccacat ggaatggtcg atggtcgcta tctgaattcc
    15601 tggttcgaga aaaccgatgc cgtgcggctg tagagctacc ggcaggaagt taagatcaga
    15661 agcgtaaccg agcagatact gatgaacgcg cagatcatcc ggcacgctac catttgcgcg
    15721 gatccacacc tgacgatgcg gttctgcgac gtgccctttc agtgggttat gaaactccac
    15781 cggacggact tcaagcggac gatcgcagat gaatttatct ttcagcactg gcggcagcag
    15841 gtgcgccagc gattgggcga tttgcgtttc cgaaggcagg c
//
LOCUS       DAMZFT010000114        15876 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.114, whole genome shotgun sequence.
ACCESSION   DAMZFT010000114 DAMZFT010000000
VERSION     DAMZFT010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15876)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15876)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15876
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.114"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(<1..431)
                     /locus_tag="PN977_003741"
     CDS             complement(<1..431)
                     /locus_tag="PN977_003741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001170212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6043740.1"
                     /translation="MQKKTLLSACIALALSGSGWAADIDDTDSATRQRKETRIPCPTA
                     HSSEKLSPQQLKSLPSECSTTNDNNLYSLIAVGATSLITTLAVLELNHDDGNHAHSSD
                     NPPVPPDDDNGGNTPDDGGNTPDDGGNTPDDGGNTPDDGGNT"
     gene            complement(889..1212)
                     /locus_tag="PN977_003742"
     CDS             complement(889..1212)
                     /locus_tag="PN977_003742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbL family protein"
                     /protein_id="HDI6043741.1"
                     /translation="MKRTLLLCAFLVGLVSSNVMALTLDEARTQGRVGETFYGYLVAL
                     KTDAETEKLVTDINAERKASYQQLAKQNNVSVDDIARLAGQKLVSRAKSGEYVQGING
                     KWLQK"
     gene            complement(1220..1405)
                     /locus_tag="PN977_003743"
     CDS             complement(1220..1405)
                     /locus_tag="PN977_003743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnbE family lipoprotein"
                     /protein_id="HDI6043742.1"
                     /translation="MKILLAALTSSFMLVGCTPRIEVAAPKEPITINMNVKIEHEIII
                     KADKDVEELLETRSDLF"
     gene            complement(1402..4041)
                     /locus_tag="PN977_003744"
     CDS             complement(1402..4041)
                     /locus_tag="PN977_003744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbH family protein"
                     /protein_id="HDI6043743.1"
                     /translation="MLGKYKAVLALLLLIILVPLTLLMTLGLWVPTLAGIWLPLGTRI
                     ALDESPRITRKGLIIPDLRYLVGDCQLAHITNASLSHPSRWLLNVGTVELDSACLAKL
                     PQTEQSPAAPKTLAQWQAMLPNTWINIDKLIFSPWQEWQGKLSLALTSDIQQLRYQGE
                     KVKFQGQLKGQQLTVSELDVVAFENQPPVKLVGEFTMPLVPDGLPVSGHATATLNLPQ
                     EPSLVDAELDWQENSGQLIVLARDNGDPLLDLPWQITRQQLTVSDGRWSWPYAGFPLS
                     GRLGVKVDNWQAGLENALVSGRLSVLTQGQAGKGNAVLNFGPGKLSMDNSQLPMQLTG
                     EAKQADLILYARLPAQLSGSLSDPTLTFEPGALLRSKGRVIDSLDIDEIRWPLAGVKV
                     TQRGIDGRLQAILQAHENELGDFVLHMDGLANDFLPDAGRWQWRYWGKGSFTPMNATW
                     DVAGKGEWHDSTITLTDLSTGFDQLQYGTMTVEKPRLILDKPVVWVRDAQHPSFSGAL
                     SLDAGQTLFTGGSVLPPSTLKFSVDGRDPTYFLFKGDLHAGEIGPVRVNGRWDGIRLR
                     GNAWWPKQSLTVFQPLVPPDWKMNLRDGELYAQVAFSAAPEQGFRAGGHGVLKGGSAW
                     MPDNQVNGVDFVLPFRFADGAWHLGTRGPVTLRIAEVINLVTAKNITADLQGRYPWSE
                     DEPLLLTDVSVDVLGGNVLMKQLRMPQHDPALLRLNNLSSSELVSAVNPKQFAMSGAF
                     SGALPLWLNNEKWIVKDGWLANSGPMTLRLDKDTADAVVKDNMTAGSAINWLRYMEIS
                     RSSTKINLDNLGLLTMQANITGTSRVDGKSGTVNLNYHHEENIFTLWRSLRFGDNLQA
                     WLEQNARLPGNDCPQGKECEEKQ"
     gene            4249..5238
                     /gene="ldhA"
                     /locus_tag="PN977_003745"
     CDS             4249..5238
                     /gene="ldhA"
                     /locus_tag="PN977_003745"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HDI6043744.1"
                     /translation="MKLAVYSTKQYDKKYLQQVNESFGFELEFFDFLLTEKTAKTANG
                     CEAVCIFVNDDGSRPVLEELKKHGVKYIALRCAGFNNVDLDAAKELGLKVVRVPAYDP
                     EAVAEHAIGMMMTLNRRIHRAYQRTRDANFSLEGLTGFTMYGKTAGVIGTGKIGVAML
                     RILKGFGMRLLAFDPYPSAAALELGVEYVDLPTLFSESDVISLHCPLTPENYHLLNEA
                     AFDQMKNGVMIVNTSRGALIDSQAAIEALKNQKIGSLGMDVYENERDLFFEDKSNDVI
                     QDDVFRRLSACHNVLFTGHQAFLTAEALTSISQTTLQNLSNLEKGETCPNELV"
     gene            5349..5771
                     /gene="hslJ"
                     /locus_tag="PN977_003746"
     CDS             5349..5771
                     /gene="hslJ"
                     /locus_tag="PN977_003746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HslJ"
                     /protein_id="HDI6043745.1"
                     /translation="MKKVAAFVALSLLMAGCVSNDKIAVTPEQLQHHRFVLESVNGKP
                     VTSDKNPPEISFGEKMMISGSMCNRFSGEGKLSNGELTAKGLAMTRMMCANPQLNELD
                     NTISEMLKEGAQVDLTANQLTLATAKQTLTYKLADLMN"
     gene            complement(5768..6034)
                     /locus_tag="PN977_003747"
     CDS             complement(5768..6034)
                     /locus_tag="PN977_003747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015964583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing protein"
                     /protein_id="HDI6043746.1"
                     /translation="MRAAFWVGCAALLLSACSSEPVQQATAAHVAPGLKASMSSSGEA
                     NCAMIGGSLSVARQLDGTAIGMCALPNGKRCSEQSLAAGSCGSY"
     gene            6308..9832
                     /gene="nifJ"
                     /locus_tag="PN977_003748"
     CDS             6308..9832
                     /gene="nifJ"
                     /locus_tag="PN977_003748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HDI6043747.1"
                     /translation="MITIDGNGAVASVAFRTSEVIAIYPITPSSTMAEQADAWAGNGL
                     KNVWGDTPRVVEMQSEAGAIATVHGALQTGALSTSFTSSQGLLLMIPTLYKLAGELTP
                     FVLHVAARTVATHALSIFGDHSDVMAVRQTGCAMLCAANVQEAQDFALISHIATLKSR
                     VPFIHFFDGFRTSHEINKIVPLADDTILDLMPQAEIDAHRARALNPEHPVIRGTSANP
                     DTYFQSREATNPWYNAVYDHVEQAMNDFAAATGRQYQPFEYYGHPQAERVIILMGSAI
                     GTCEEVVDELLTRGEKVGVLKVRLYRPFSAKHLLQALPGSVRSVAVLDRTKEPGAQAE
                     PLYLDVMTALAEAFNNGERETLPRVIGGRYGLSSKEFGPDCVLAVFAELNAAKPKARF
                     TVGIYDDVTNLSLPLPENTLPNSAKLEALFYGLGSDGSVSATKNNIKIIGNSTPWYAQ
                     GYFVYDSKKAGGLTVSHLRVSEQPIRSAYLISQADFVGCHQLQFIDKYQMVERLKPGG
                     IFLLNTPYSADEVWSRLPQEVQAVLNQKKARFYVINAAKIARECGLAARINTVMQMAF
                     FHLTQILPGDSALAELQGAIAKSYSSKGQDLVERNWQALALARESVEEVPLQPVNPHS
                     ANRPPVVSDAAPDFVKTVTAAMLAGLGDALPVSALPPDGTWPMGTTRWEKRNIAEEIP
                     IWKEELCTQCNHCVAACPHSAIRAKVVPPEAMENAPASLHSLDVKSRDMRGQKYVLQV
                     APEDCTGCNLCVEVCPAKDRQNPEIKAINMMSRLEHVEEEKINYDFFLNLPEIDRSKL
                     ERIDIRTSQLITPLFEYSGACSGCGETPYIKLLTQLYGDRMLIANATGCSSIYGGNLP
                     STPYTTDANGRGPAWANSLFEDNAEFGLGFRLTVDQHRVRVLRLLDQFADKIPAELLT
                     ALKSDATPEIRREQVAALRQQLNDVAEAHELLRDADALVEKSIWLIGGDGWAYDIGFG
                     GLDHVLSLTENVNILVLDTQCYSNTGGQASKATPLGAVTKFGEHGKRKARKDLGVSMM
                     MYGHVYVAQISLGAQLNQTVKAIQEAEAYPGPSLIIAYSPCEEHGYDLALSHDQMRQL
                     TATGFWPLYRFDPRRADEGKLPLALDSRPPSEALEETLLHEQRFRRLNSQQPEVAEQL
                     WKDAAADLQKRYDFLAQMAGKAEKSNTD"
     gene            10198..11316
                     /gene="ompC"
                     /locus_tag="PN977_003749"
     CDS             10198..11316
                     /gene="ompC"
                     /locus_tag="PN977_003749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HDI6043748.1"
                     /translation="MKSKVLALLIPALLAAGAAHAAEVYNKDGNKLDLYGKVDGLHYF
                     SDNTNSDGDQTYVRFGFKGETQITDQLVGYGQWEYNVQANGSEGDKGDSWTRLAFAGL
                     KFGDLGSFDYGRNYGVMYDIEGWTDMLPEFGGDSYTKADNYMTGRANGVATYRNTDFF
                     GLVDGLNFALQYQGANENQVTHEQEGTANGGDRNIKNANGDGFGISTTYDLGMGMSFG
                     AAYTSSDRTNQQAEFSTAKGDKADAWTAGAKYDANNIYLATMYSETRNMTPYGDSTNT
                     IANKTQNFEVTAQYQFDFGLRPAVSFLMSKGKDLVGTDGDKDLVKYADVGATYYFNKN
                     MSTYVDYKINLLDEDDSFYAANGISTDDIVALGLVYQF"
     gene            11457..11891
                     /gene="uspF"
                     /locus_tag="PN977_003750"
     CDS             11457..11891
                     /gene="uspF"
                     /locus_tag="PN977_003750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310024.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspF"
                     /protein_id="HDI6043749.1"
                     /translation="MNRTILVPIDISDSELTQRVISHVEAEAKIDDAEVHFLTVIPSL
                     PYYASLGLAYSAELPAMDDLKAEAKSQLEEIIKKFKLPTDRVHVHVEEGSPKDRILEL
                     AKKIPAHMIIIASHRPDITTYLLGSNAAAVVRHAECSVLVVR"
     gene            12067..13002
                     /gene="ttcA"
                     /locus_tag="PN977_003751"
     CDS             12067..13002
                     /gene="ttcA"
                     /locus_tag="PN977_003751"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HDI6043750.1"
                     /translation="MSQNQEISKKEQYNLNKLQKRLRRNVGEAIADFNMIEEGDRIMV
                     CLSGGKDSYTMLEILRNLQQSAPINFSLVAVNLDQKQPGFPEHVLPEYLETLGVEYKI
                     VEENTYGIVKEKIPEGKTTCSLCSRLRRGILYRTATELGATKIALGHHRDDILQTLFL
                     NMFYGGKMKGMPPKLMSDDGKHIVIRPLAYCREKDIQRFADAKAFPIIPCNLCGSQPN
                     LQRQVIADMLRDWDKRYPGRIETMFSAMQNVVPSHLCDTNLFDFKGITHGSEVVNGGD
                     LAFDREEIPLQPAGWQPEEDENQLDELRLNVVEVK"
     gene            complement(13131..14504)
                     /gene="dbpA"
                     /locus_tag="PN977_003752"
     CDS             complement(13131..14504)
                     /gene="dbpA"
                     /locus_tag="PN977_003752"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DbpA"
                     /protein_id="HDI6043751.1"
                     /translation="MTAFSTLNVLPPAQLTNLNELGYLTMTPVQAAALPAILAGKDVR
                     VQAKTGSGKTAAFGLGLLQQIDASLFQTQALVLCPTRELADQVAGELRRLARFLPNTK
                     ILTLCGGQPFGMQRDSLQHAPHIIVATPGRLLDHLQKGTVSLDALNTLVMDEADRMLD
                     MGFSDAIDDVIRFAPASRQTLLFSATWPEAIAAISGRVQRDPLAIEIDSTDALPPIEQ
                     QFYETFSKGKIPLLQRLLSLHQPSSCVVFCNTKKDCQAVCDALNEVGQSALSLHGDLE
                     QRDRDQTLVRFANGSARVLVATDVAARGLDIKSLELVVNFELAWDPEVHVHRIGRTAR
                     AGNSGLAISFCAPEEAQRANIISDMLQIKLNWQTPPANSSIVPLEAEMATLCIDGGKK
                     AKMRPGDVLGALTGDIGLDGADIGKIAVHPAHVYVAVRQAVAHKAWKQLQGGKIKGKT
                     CRVRLLK"
     gene            complement(14982..>15876)
                     /gene="zntB"
                     /locus_tag="PN977_003753"
     CDS             complement(14982..>15876)
                     /gene="zntB"
                     /locus_tag="PN977_003753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="zinc transporter ZntB"
                     /protein_id="HDI6043752.1"
                     /translation="NTDVIDEAHPCWLHLNYVHHDSAQWLATTPLLPNNVRDALAGES
                     TRPRVSRLGEGTLITLRCINGSTDERPDQLVAMRVYMDGRLIVSTRQRKVLALDDVVS
                     DLEEGTGPTDCGGWLVDVCDALTDHSSEFIEQLHDKIIDLEDNLLDQQIPPRGFLALL
                     RKQLIVMRRYMAPQRDVYARLASERLPWMSDDQRRRMQDIADRLGRGLDEIDACIART
                     GVMADEIAQVMQENLARRTYTMSLMAMVFLPSTFLTGLFGVNLGGIPGGGWQFGFSIF
                     CILLVVLIGGVALWLHRSKWL"
BASE COUNT         4066 a         4019 c         3844 g         3947 t
ORIGIN      
        1 gtgttgccac catcatccgg tgtgttgccg ccatcgtcag gagtattccc accatcgtca
       61 ggggtattgc cgccatcgtc cggtgtgttg ccgccattat cgtcatcagg cggtactgga
      121 ggattgtcag aggaatgagc gtggttaccg tcatcgtggt ttagttcaag gactgcaaga
      181 gtggtgatta gtgaagtagc gccaacggca atcaaggaat agaggttgtt gtcattggtt
      241 gtagaacatt cagacgggag cgattttagt tgttgcggac ttagtttttc agaggagtga
      301 gcggtcggac agggtatcct tgtctcctta cgctgacgag ttgcactatc tgtatcatca
      361 atgtcagcag cccagcccga tccgcttaaa gccagcgcga tacaggcgga taataacgtt
      421 ttcttttgca tgagaaatcc ctctcaatca gagacagttt aaacagtaaa atgggtgtaa
      481 cagaaacgtc ataaacgaag agtggtcatg attatatatt atttaagaag ctttatttag
      541 caattcatga caattcaatt catcatcaaa taactttaat atataaccca gatgaatgtt
      601 ttattttatt aatcaggtcg ctcgttacat tacattaaat tatttcttct aacaaatttt
      661 acacgaacag taaaatggat ggcaggttgc tttcaccacc caattccgta aattcacaca
      721 tattcgaaat atcaattctt attattttac ttgaaccgat aataaacgca gaaattctat
      781 tttgtagctc attgttgcct cctgtgtgaa ttgttcgaat ctaccaggct gcgatgaaag
      841 aagtcgtgta gtagagaact atacccgcaa caagcggggc agacgttctt atttctgtaa
      901 ccattttccg ttaatgccct gcacatattc tcctgattta gcgcgagata cgagcttttg
      961 ccccgccagt ctcgcgatat catctaccga cacattattt tgctttgcca gttgttggta
     1021 actcgcttta cgttcggcat taatgtcggt tactaatttc tctgtttcag catccgtttt
     1081 cagcgcaacc agataaccat aaaatgtttc acctacccgc ccctgagttc tggcttcatc
     1141 cagagtcaat gccattacat tgctgcttac caagccaaca agaaatgcac aaagaagtaa
     1201 tgttcttttc atcatcacct caaaaaagat cgctacgggt ctcaagcagc tcttcgacat
     1261 ctttgtctgc cttgatgatg atctcatgct caattttaac gttcatattg atagtgatcg
     1321 gttccttagg ggcagcgact tcaatgcgag gcgtacagcc aaccagcata aaagatgacg
     1381 tcaacgcagc cagtaaaatt ttcattgttt ttcctcacac tcttttcctt gcggacagtc
     1441 atttcccggc agacgtgcgt tctgctccaa ccatgcctgg agattatcgc caaagcgtaa
     1501 actgcgccac agcgtaaaaa tattctcttc atgatggtaa ttaagattta ccgtaccgct
     1561 tttaccatca acgcgactgg tacctgtaat gttggcctgc atggttaata aaccgagatt
     1621 atctaaatta atttttgtcg atgaacggct aatttccata tagcgcaacc agttaattgc
     1681 tgaacccgca gtcatattgt ctttcaccac cgcatctgcg gtgtctttat ccagccgcaa
     1741 ggtcattggc ccgctatttg ccagccaacc atctttcact atccattttt cgttgtttag
     1801 ccataacggc aatgcaccac taaatgcccc ggacatggcg aattgtttcg gattgacggc
     1861 gctaaccagt tcgctggagg aaagattatt cagccgcaac agcgccgggt catgttgcgg
     1921 catacgtaat tgtttcatca gcacgttacc gcctaacaca tcgacgctaa catcggtcaa
     1981 caacaatggt tcgtcttcac tccacggata acgcccttgc aaatcagccg taatattttt
     2041 cgctgtcacc agattaatca cttcggcaat tcgcaacgta acggggccgc gagtccccag
     2101 atgccaggct ccatcggcaa aacggaaagg caggacaaaa tcgacaccgt taacctgatt
     2161 atctggcatc caggcactac cgcctttcaa cacgccgtgc cctcccgcgc ggaatccttg
     2221 ttcaggcgca gcagaaaatg cgacctgggc atatagttcg ccatcgcgta agttcatctt
     2281 ccagtcgggt ggcaccagcg gctggaatac ggtcagtgat tgtttaggcc accaggcgtt
     2341 accgcgcaga cgaataccgt cccagcgacc atttacccga accgggccaa tctcaccagc
     2401 atgtaaatcg cctttaaaga ggaaatacgt cggatcgcgt ccatcgacgc taaattttaa
     2461 ggtcgatggc ggtaacacac tgccgccagt gaacagcgtt tgcccggcgt ccagtgacag
     2521 cgcgccgcta aaggagggat gctgtgcgtc acgtacccag acgacgggct tgtcgagaat
     2581 tagtcgcggc ttttctaccg tcatcgtacc gtattgtaac tggtcgaaac cggtggagag
     2641 atcggtcagc gtaatcgtgc tgtcatgcca ctcacccttt cctgcgacat cccaggtggc
     2701 attcatcggt gtaaaactgc cttttcccca gtagcgccac tgccagcggc cagcgtcagg
     2761 gagaaaatca ttcgccagtc catccatatg cagcacgaaa tcgcccagtt cattttcatg
     2821 cgcctgcaaa atggcctgca aacgtccgtc aataccacgt tgggtgactt ttacacccgc
     2881 taaaggccag cggatttcat cgatgtccag cgaatcaatg actcttccct ttgaacgaag
     2941 taacgcgcct ggctcaaagg tcaacgttgg gtcagacaga cttccactta gctgcgcagg
     3001 taaacgggca tataaaatga ggtccgcctg tttcgcttca ccggtcagtt gcataggcag
     3061 ctggctgtta tccatgctta attttcctgg gccaaaatta agcaccgcgt tgcccttacc
     3121 cgcttgcccc tgggtcagca cactcagtcg tccgctgacc agagcgttct cgagccctgc
     3181 ctgccagttg tcgactttga cacccagtcg gccactcaaa ggaaaacctg catacggcca
     3241 gctccagcga ccatcgctta cggtcaattg ttgacgagta atttgccacg gcaaatcgag
     3301 caacggatcg ccgttatccc gtgccagcac gatcaattgc ccgctatttt cctgccagtc
     3361 cagctctgca tccaccagtg acggttcctg cggcaagttt aacgtcgcag tagcatgccc
     3421 acttaccgga agtccatccg gcacgagcgg catagtaaat tcccccacca gtttaaccgg
     3481 cggctgattt tcaaacgcga cgacatccag ttcgctgact gtaagttgtt gccctttcag
     3541 ctggccttga aatttaactt tttcgccctg ataacgcagt tgctggatat cggaggttaa
     3601 tgcgagagag agttttccct gccattcctg ccagggagaa aaaatcagtt tatcgatatt
     3661 gatccaggtg ttaggcaaca tggcctgcca ctgcgcgagg gtttttggag cggctggcga
     3721 ttgctccgtc tgcggcaatt tcgccagaca agcagaatca agttctaccg tgccgacgtt
     3781 caataaccag cggctgggat gtgaaaggct ggcgttggtg atatgcgcaa gctgacaatc
     3841 tcccaccaga taacggagat cgggaatgat taaaccttta cgcgtaatgc gtgggctttc
     3901 atctaatgca atacgtgtcc cgagcggtag ccagatgccc gccagcgtag gaacccacag
     3961 cccgagcgtc atcagcagcg tcaacggcac aagaataatc agtaataaca gcgcgagaac
     4021 ggctttatat ttacccagca tgggtagtta atatcctgat ttagcgaaaa attaagcatt
     4081 caatacgggt attgtggcat gtttaaccgt tcagttgaag gttgcgccta cactaagcat
     4141 agttgttgat gaatttttca atatcgccat agctttcaat tatatttgaa attttgtaaa
     4201 atatttttag tagcttaaat gtgattcaac atcactggag aaagtcttat gaaactcgcc
     4261 gtttatagca caaaacagta cgacaagaag tacctgcaac aggtgaacga gtcctttggc
     4321 tttgagctgg aattttttga ctttctgctg acggaaaaaa ccgctaaaac tgccaatggc
     4381 tgcgaagcgg tatgtatttt cgtaaacgat gacggcagcc gcccggtgct ggaagagctg
     4441 aaaaagcacg gcgttaaata tatcgccctg cgctgtgccg gtttcaataa cgtcgacctt
     4501 gacgcggcaa aagaactggg gctgaaagta gtccgtgttc cagcctatga tccagaagcc
     4561 gttgctgaac atgccatcgg tatgatgatg acgctgaacc gccgtattca ccgcgcgtat
     4621 cagcgtaccc gtgacgccaa cttctctctg gaaggtctga ccggctttac tatgtatggc
     4681 aaaacggcag gcgttatcgg tactggtaaa atcggtgtgg cgatgctgcg cattctgaaa
     4741 ggttttggta tgcgtctgct ggcgttcgat ccgtatccaa gtgcggcggc gctggaactc
     4801 ggtgtggagt atgtcgatct gccaaccctg ttctctgaat cagacgttat ctctctgcac
     4861 tgcccgctga caccggaaaa ctatcatctg ttgaacgaag ccgccttcga tcagatgaaa
     4921 aatggcgtga tgatcgtcaa taccagtcgc ggtgcattga ttgattctca ggcagcaatt
     4981 gaagcgctga aaaatcagaa aattggttcg ttgggtatgg acgtgtatga gaacgaacgc
     5041 gatctgttct ttgaagacaa atccaacgac gtgatccagg atgacgtatt ccgtcgtctg
     5101 tctgcctgcc acaacgtgct gtttaccggg caccaggcat tcctgacagc agaagcactg
     5161 accagtattt ctcagactac gctgcaaaac ttaagcaatc tggaaaaagg cgaaacctgc
     5221 ccgaacgaac tggtttaatc ttgccgctcc cctgcattcc aggggagctt attcagataa
     5281 tccccaaaga cctttcatcc tctattctta aaatagtcct gagtcagaaa ctgtaattga
     5341 gaaccacaat gaagaaagta gccgcgtttg ttgcgctaag cctgctgatg gcgggatgtg
     5401 taagtaatga caaaattgct gtaaccccag aacagctaca gcatcatcgc tttgtgctgg
     5461 aaagcgtaaa cggtaagccc gtgaccagcg ataaaaatcc gccagaaatc agctttggtg
     5521 aaaagatgat gatttccggc agcatgtgta accgctttag cggtgaaggc aaactgtcta
     5581 atggtgaact gacagccaaa ggactggcaa tgacccgtat gatgtgcgct aacccgcagc
     5641 ttaatgaact cgataacacc attagcgaaa tgctgaaaga aggtgcacaa gtggatctga
     5701 ccgcgaacca gttaacgctg gcgaccgcga aacagacatt aacttataag ctggcggatt
     5761 taatgaatta atagctgcca cagctcccgg cggcaagtga ctgttcgcta cagcgtttgc
     5821 cgttgggtaa tgcacacatc ccaatcgccg taccatccag ttgacgggca acagaaagcg
     5881 aaccgccgat cattgcacaa tttgcttctc cactactgga catcgacgct tttaaacctg
     5941 gcgctacgtg cgccgcagtc gcctgctgaa caggttcact actacacgcc gacaataata
     6001 aagcggcaca ccctacccaa aacgctgctc gcatcttttc ttcctctgat cttcaagcca
     6061 aacgacaccg ccataaataa taggcagcac agagggcggc gtcgagagct gtcctgcgcg
     6121 ttgccccgca tttttacttt tttatggcta tttttttgcc ctctgtttga tcaaaacatt
     6181 cattacgctg atgtggggga cacaaaagcg aaaatgcaga agaaagccat ttgctaaaat
     6241 tgaaagataa ctactgggcg cgcagcaatt tcgtgcgccc ctcatttgcg caatgtaagg
     6301 gtgtcatatg attactattg acggtaatgg cgcggttgct tcggtggcgt ttcgcaccag
     6361 tgaagttatc gccatctacc ctattacccc cagttccacg atggcagaac aggctgatgc
     6421 ctgggccgga aacggcttga agaacgtttg gggagacaca ccacgcgtgg ttgaaatgca
     6481 gtcggaagcg ggtgctatcg ctaccgtgca tggcgctttg cagacgggtg ccctttcaac
     6541 atcgtttacg tcatcgcagg gtttgctgct gatgatcccg acgctgtaca aactggcagg
     6601 cgaactcaca ccgtttgtcc tgcatgtagc ggcacgtacc gttgccacac atgcactctc
     6661 tatttttggc gatcattccg acgttatggc ggtgcgccag acgggttgcg cgatgttgtg
     6721 tgcggctaac gtccaggaag cgcaagactt tgctctcatt tcgcacatcg caacgctgaa
     6781 gagccgcgtg ccatttattc atttctttga tggtttccgc acgtcccacg aaatcaataa
     6841 aattgtcccg ctggccgatg acactattct tgatctcatg ccgcaggccg aaattgatgc
     6901 tcatcgcgcc cgggcgctca acccggaaca tccggtgatc cgcggtacgt ccgccaatcc
     6961 tgacacttat ttccagtctc gcgaagccac caacccctgg tacaacgcgg tctatgacca
     7021 tgttgaacag gcgatgaatg atttcgctgc cgcgacaggt cgtcagtatc agccatttga
     7081 gtattatggg catccgcaag cggaacgggt gattatcctg atgggctcag ccattggcac
     7141 ctgtgaagaa gtggttgatg aattgctaac ccgtggcgaa aaagtcggcg tgctgaaagt
     7201 tcgcctgtac cgtcccttct ccgccaaaca cttgctacaa gctctgccgg gatccgtacg
     7261 cagcgtggcg gtactggaca gaaccaaaga acccggtgcc caggcagaac cgctctatct
     7321 ggatgtgatg accgcactgg cagaagcctt taataatggc gagcgcgaaa ccctgccccg
     7381 tgtcattggt gggcgctatg gtctttcatc caaagaattt ggcccggact gtgtactggc
     7441 ggtatttgcc gagctcaacg cggctaaacc gaaagcgcgc tttacggttg gtatttacga
     7501 tgatgtgacc aatctgtcac tgccgttgcc ggaaaacacc ctgccaaact cggcgaaact
     7561 tgaagccttg ttttatggcc ttggtagtga tggcagcgtt tccgcgacca aaaacaatat
     7621 caagattatc ggtaattcca cgccgtggta cgcacagggc tattttgttt acgactccaa
     7681 aaaggcgggc ggcctgacgg tttctcacct tcgcgtgagc gagcagccga ttcgttccgc
     7741 ttatctgatt tcccaggctg attttgttgg ctgccaccag ttgcagttta tcgacaaata
     7801 tcagatggtt gagcgtttaa aacctggcgg cattttcctg ctcaacacgc cgtacagcgc
     7861 agatgaagtg tggtcgcgct tgccgcagga agttcaggcc gtgttaaacc agaaaaaagc
     7921 gcgcttctat gtgattaacg cggcgaaaat cgcccgcgaa tgtggcctgg cggcccgaat
     7981 taataccgtc atgcagatgg cttttttcca tctgacgcaa attctgcctg gcgatagcgc
     8041 cctcgcagaa ttgcagggtg cgattgccaa aagttacagt agcaaaggtc aggatctggt
     8101 ggagcgcaac tggcaggctc tggcgctggc ccgtgaatcc gtagaagaag ttccgctgca
     8161 accagtaaat ccgcacagcg ccaatcgacc gccagtggtt tccgatgccg cccctgattt
     8221 cgtgaaaacc gtaaccgcgg cgatgctcgc cgggcttggt gatgccctcc ccgtttcggc
     8281 gctgccgcca gacggcacct ggccgatggg caccacgcgc tgggaaaaac gcaatatcgc
     8341 cgaagagatc cccatctgga aagaggaact ctgtacccaa tgtaaccact gcgtcgctgc
     8401 ttgcccacac tcagctattc gcgcaaaagt ggtaccgcct gaagcgatgg aaaacgcccc
     8461 tgccagcctg cattcgctgg atgtgaaatc gcgtgatatg cgcgggcaga aatatgtctt
     8521 gcaggtggct ccggaagatt gcaccggttg taacctgtgc gtcgaagttt gcccggcgaa
     8581 agaccgtcag aatccagaga ttaaagccat caatatgatg tcgcggctgg aacatgtcga
     8641 agaagagaaa atcaattacg atttcttcct caacctgcca gaaatcgacc gtagcaaact
     8701 ggaacgtatt gatattcgta catcacagct gattacaccg ctgtttgaat attccggtgc
     8761 ttgctccggt tgtggcgaga cgccgtatat taaattgctg actcagctct atggcgatcg
     8821 gatgttgatc gctaacgcta cgggttgttc ttcaatttat ggtggtaacc tgccctccac
     8881 accatatacc actgatgcca acggtcgtgg accggcatgg gcgaactcgc tgtttgaaga
     8941 taacgccgaa ttcggccttg gtttccgcct tacggtcgat caacaccgtg tccgcgtgct
     9001 gcgtctgctg gatcaatttg ccgataaaat cccggcggaa ttactgactg cgttgaaatc
     9061 agacgctacg ccagagattc gtcgtgaaca ggttgcagct ttacgccagc aactcaacga
     9121 tgttgccgaa gcacatgaac tgctgcgtga cgcagatgct ctggtggaaa aatcaatctg
     9181 gctgattggt ggcgatggct gggcttacga tattggcttt ggcggtctgg atcatgtatt
     9241 gagcctgacg gaaaacgtca acattctggt gctggatacg caatgctatt ccaacactgg
     9301 tggtcaggcg tcgaaagcga caccgctggg tgcagtaact aagttcggcg agcacggcaa
     9361 acgtaaagcg cgtaaagatc ttggcgtcag tatgatgatg tacggtcatg tttatgtggc
     9421 gcagatttct ctcggcgcgc agctgaacca gacggtgaaa gcgattcagg aagcggaagc
     9481 gtatccggga ccatcgctga tcattgctta tagcccgtgt gaagagcatg gttacgatct
     9541 ggctctcagc cacgaccaga tgcgccaact cactgctacc ggcttctggc cgctatatcg
     9601 ctttgatcca cgtcgagccg atgaaggcaa actgccgctg gcactggatt cacgcccgcc
     9661 gtcagaagca ctggaagaaa cgttacttca tgagcaacgt ttccgtcgtc tgaattcgca
     9721 gcagccagaa gtggcagaac agttatggaa agatgctgca gctgatttgc aaaaacgcta
     9781 tgacttcctg gcacaaatgg ccggaaaagc ggaaaaaagc aacaccgatt aaatgctctg
     9841 gataaggatt atccaattct aaaaaaaagc ccggacgact gttcgggctt gtctttttat
     9901 atgttggaaa atcagtggca atgcaatggc ccaacagaaa ataaaatctg tgacaataaa
     9961 ggcatataac ccgcgaagaa taacgtatag caaattaaaa gtataatttt tattttgtat
    10021 gtattatctt cgatacattc accagactta ttatcatttc agaggaatta tttgattaag
    10081 gttttactta aggcgtaaca aatgatattt atcggctaac tgaacttctc ctttaggatg
    10141 ttttcactcc ccgaaatggg gataaaaggc aaataaaata actaaaggat ttattcaatg
    10201 aaaagcaaag ttctggcact tttaattcct gccctgctcg ccgcaggtgc tgcacatgca
    10261 gccgaagttt ataataaaga cggcaacaaa ttagatctgt atggcaaagt tgatggcctg
    10321 cattattttt ctgataatac caatagtgat ggtgatcaaa cttacgtgcg ttttggtttt
    10381 aagggtgaaa cgcaaatcac ggatcaactg gttgggtatg gccagtggga atataacgtg
    10441 caggctaatg gctctgaagg tgataaaggt gattcctgga ctcgtcttgc ttttgccggt
    10501 ctgaaatttg gtgacctggg ttcattcgac tatggtcgta attatggcgt aatgtacgac
    10561 atcgaaggct ggaccgatat gctaccggaa ttcggtggtg actcttatac caaagccgat
    10621 aactatatga ccggtcgagc caatggcgta gcaacctatc gtaatactga tttcttcggt
    10681 ctggttgatg gtctgaattt tgcgttacag taccagggcg cgaatgaaaa ccaggtcacc
    10741 catgagcaag aaggtaccgc aaacggtggt gatcgaaaca ttaagaacgc caatggcgac
    10801 ggttttggca tctccactac ctacgactta ggtatgggca tgagttttgg tgccgcctat
    10861 accagctccg atcgcactaa ccagcaggct gaattctcta ccgcaaaagg cgataaggct
    10921 gacgcctgga ctgccggggc aaaatacgat gctaacaata tctacctggc gaccatgtat
    10981 tctgaaacgc gtaacatgac accttacggt gattcaacaa atacgattgc caacaaaacc
    11041 cagaactttg aagttactgc gcagtatcag ttcgattttg ggctgcgtcc ggcagtctct
    11101 ttcctgatgt ctaaaggcaa ggatttggtc ggtaccgacg gtgataaaga tctggttaaa
    11161 tacgccgatg ttggcgcgac ttactatttc aacaaaaata tgtccaccta cgttgactat
    11221 aaaatcaacc tgttggatga agatgacagc ttctacgctg ccaacggcat ctctaccgat
    11281 gatattgtcg ctttaggtct ggtttatcag ttctaaatcc tcctgcccgc tgttatggcg
    11341 ggctttttct gcttattctt cctcctttga tctaaattaa aaatgtgaac tccgtcatta
    11401 cacaaaaagt gtcatctggc gttacacttt atgcggatac taaaacagga ggttttatga
    11461 acagaacgat tcttgtccct atcgatattt ccgattcaga attaactcaa cgcgtgatta
    11521 gccacgttga ggcagaggca aagattgatg atgcagaggt tcatttcctg acggtaatac
    11581 cttcactgcc ctactatgcc tctctgggtt tagcgtattc cgcagaatta ccggcaatgg
    11641 atgacctgaa agcggaagcc aaatcgcaac tggaagagat cattaaaaaa tttaaactac
    11701 caaccgacag agtgcatgtc catgttgagg aaggctcgcc caaagaccgc attctggaat
    11761 tggcgaagaa gatccccgct catatgatca tcattgcttc ccatcgaccg gatatcacca
    11821 cttatctgct cggttctaac gccgcagctg tagtgcgtca cgcagagtgc tccgtgctgg
    11881 ttgtgcgctg acactaacgc ccgcacattg ctgcgggctt tttgattcat ttcgcaaatg
    11941 tgctgacatt ttcccctcta atccgtacca tacacgccac agtttttata tcagatttct
    12001 tatgctgggc gttccggcat gacgcatact cttctgatgc catataacga attgagtcgc
    12061 ttttaaatgt cgcaaaatca agaaattagt aagaaagaac aatacaatct gaacaaatta
    12121 caaaaacgtc tgcgtcgtaa cgtgggcgaa gccattgctg acttcaatat gattgaagaa
    12181 ggcgatcgca tcatggtttg cctctccggg ggtaaagaca gctataccat gctggaaatt
    12241 ctgcgcaatt tgcagcaaag cgcgccaatc aatttttcgc tggtggctgt taacctcgat
    12301 caaaaacaac cgggctttcc ggaacacgtt ctgcccgagt atcttgaaac gctgggcgtt
    12361 gagtacaaga ttgtggaaga gaacacttac ggtatcgtga aagagaagat tccggagggc
    12421 aaaaccactt gctcactgtg ttctcgtctt cgtcgcggta tcctttatcg caccgcaacg
    12481 gaactggggg cgacgaagat cgcgttgggt caccatcgtg acgatatcct gcaaacgctg
    12541 ttcttaaata tgttctacgg cggcaagatg aaaggtatgc ctccgaaact gatgagcgat
    12601 gatggcaaac atatcgtgat tcgcccgctg gcctactgcc gcgagaaaga tattcagcgc
    12661 tttgccgatg caaaagcgtt cccgattatt ccgtgcaacc tgtgcggttc acagcctaac
    12721 ctgcaacgtc aggtgattgc tgacatgttg cgtgactggg ataaacgtta tccggggcgt
    12781 atcgagacga tgttcagcgc gatgcagaat gtggtgccat cgcatctgtg cgataccaac
    12841 ctgttcgatt tcaaaggcat tacccacggt tctgaagtgg ttaacggggg tgatctggcg
    12901 tttgatcgcg aagagatccc tctacaaccg gcgggttggc agcctgaaga agatgaaaat
    12961 cagttggatg agttacgact gaatgtggtt gaagtgaaat aacccggata gcgcccgatg
    13021 cgcaagctta tcgggctact cttatggagg ccggagaaga cgcggccagc gtcgcatccg
    13081 gcaatcccga agaagatatt gactcttgca ccctgcgatt caacatttca ttattttaat
    13141 aaccgcaccc ggcacgtttt tcctttaatc ttcccgccct gtaactgttt ccatgcttta
    13201 tgagcaacag cctgacgcac cgcgacatag acatgcgccg gatgcacggc gattttgcca
    13261 atatctgcgc catcaagccc gatatctcct gtcagcgcac ctaatacatc acccgggcgc
    13321 attttggctt ttttcccgcc atcgatacat aacgttgcca tttcggcttc cagcggcaca
    13381 atggaactat tagctggcgg cgtttgccag ttaagtttta tctgcaacat gtcagaaatg
    13441 atattggccc gctgtgcttc ttccggggca cagaaactga tcgccagacc gctatttcct
    13501 gcacgcgctg tacgaccgat gcgatgtaca tgaacttcag ggtcccacgc cagttcaaag
    13561 ttcactacca gctcaagcga tttaatatcc agaccacgcg cagcaacatc agtcgcgacc
    13621 agtacacggg cgctaccgtt agcaaaacgt accagagtct gatcgcgatc gcgttgctcc
    13681 agatcgccgt gtaacgacaa tgcactttgc cctacttcat tcagcgcgtc acagacagcc
    13741 tggcaatctt ttttggtatt gcaaaacacc acgcaagagg atggctgatg caaacttaat
    13801 aaccgttgca acaacggaat tttgcctttg ctgaatgtct cataaaattg ttgttcaatg
    13861 ggaggcagag catctgttga gtcaatttca atcgctaaag gatcgcgttg cactcgtccg
    13921 ctgattgcgg cgatggcttc cggccaggtt gccgaaaaca gaagcgtctg tcgagatgct
    13981 ggcgcaaaac ggatgacatc atcaatggcg tcgctaaatc ccatatccag catgcggtcg
    14041 gcctcatcca tcaccaaagt attcagcgca tccagtgata ccgtgccttt ttgcaagtga
    14101 tccagcaaac gacccggcgt tgccacgata atatgcggcg catgttgcaa cgaatcacgc
    14161 tgcataccga acggttgacc accgcacaac gtcaaaattt tggtatttgg cagaaaacgc
    14221 gccagccgac gcaattcacc tgccacctga tccgccagtt cacgcgtagg acacagcact
    14281 aaagcctggg tttgaaatag cgacgcatca atttgctgta acaagccgag gccaaaagcc
    14341 gccgttttgc cactgccggt tttcgcctgc acgcgaacat cttttccggc aaggatcgcc
    14401 ggaagcgcgg cggcctgcac cggcgtcatg gttaaataac ccaactcatt aaggttcgtg
    14461 agttgggcgg gaggcaaaac attcagggta gaaaaagcgg tcacaatcta ttctcgtggt
    14521 catcgacgca aagtcagcag gcacgtatcc tcgcagatct acgctcacga tgcgacaatt
    14581 taatcggttc ttcatcgggg ggtgggtcag gcatgggttg cgggcgaggg atcggatcgg
    14641 gcacaggaac aggatcgcca ggaatcggtt cagggacagg aatttgcaaa taaatgagtg
    14701 tcgtcatatt tccctccggt cattgggtgg actcttaaag ggtagacgct gatatataac
    14761 aggcaaaaaa aagccgactc atcaaagtcg gcgtcgtacg aatcaattgt gctatgcagt
    14821 aattcaaaaa aggaagtaag acaatatgga gcgcaacgcc catcgcttga cgttgcattc
    14881 acctgcaagg gagatattgc cctgaatggg tagagagttt attgacttcg ctcaaacttt
    14941 gcggcgtttt tgtatacaga cagccgggaa aattgctttt gttacaacca tttactacga
    15001 tgcaaccata aagcaacacc accaataaga acaactaaca gaatacaaaa aattgaaaat
    15061 ccgaattgcc acccgccgcc agggatccca ccaaggttga cgccaaataa cccggtcaga
    15121 aaggtactgg gtaaaaagac cattgccatc aacgacattg tataggtacg acgcgctaaa
    15181 ttttcctgca tcacctgagc gatttcatcc gccatcacgc cagtccgtgc tatacaggcg
    15241 tcgatttcgt caaggccgcg cccaaggcga tcggcaatat cctgcatccg acggcgttgg
    15301 tcatcgctca tccacggcaa acgttcactg gcaagacgag cataaacatc acgttgcggt
    15361 gccatatagc gacgcatcac aattaattgt ttgcgcagca gagccaggaa tccacgcggt
    15421 ggaatttgct gatcaaggag attatcttca aggtcgataa ttttatcgtg cagctgctcg
    15481 ataaattcgc tggaatgatc ggtcaacgca tcgcacacat ccaccagcca tcccccgcaa
    15541 tcggtcggac ccgtgccctc ttccagatcg cttaccacat cgtccagcgc cagcactttg
    15601 cgttgtcggg tcgaaacaat taaccgcccg tccatatata cacgcatggc gaccagttga
    15661 tcggggcgtt catcggtgct gccgtttata cagcgcaatg taatcagcgt gccttcaccg
    15721 agacggctga ctcggggccg tgtgctctcg cccgccagcg catcacgtac gttattggga
    15781 agcagcggtg ttgtcgccag ccattgggcg ctatcatggt gtacataatt aaggtggagc
    15841 cagcagggat gcgcttcatc aatcacatct gtattt
//
LOCUS       DAMZFT010000115        15735 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.115, whole genome shotgun sequence.
ACCESSION   DAMZFT010000115 DAMZFT010000000
VERSION     DAMZFT010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15735)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15735)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15735
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.115"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            12..890
                     /locus_tag="PN977_003754"
     CDS             12..890
                     /locus_tag="PN977_003754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417628.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HDI6043753.1"
                     /translation="MKYSLGPVLWYWPKETLEEFYQQAAASSADVIYLGEAVCSKRRA
                     TKVGDWLEMAKSLAGSGKQIVLSTLALVQASSELGELKRYVENGEFLIEASDLGVVNM
                     CAERKLPFVAGHALNCYNAVTLKILLKQGMMRWCMPVELSRDWLVNLLNQCDELGIRN
                     QFEVEVLSYGHLPLAYSARCFTARSEDRPKDECETCCIKYPNGRNVLSQENQQVFVLN
                     GIQTMSGYVYNLGNELTSMQGLVDVVRLSPQGTDTFAMLDAFRANENGAAPLPLTANS
                     DCNGYWRRLAGLELQA"
     gene            971..1978
                     /locus_tag="PN977_003755"
     CDS             971..1978
                     /locus_tag="PN977_003755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HDI6043754.1"
                     /translation="MTDKTIAFSLLDLAPIPEGSSAREAFSHSLDLARLAEKHGYHRY
                     WLAEHHNMTGIASAATSVLIGYLAANTTTLHLGSGGVMLPNHSPLVIAEQFGTLNTLY
                     PGRIDLGLGRAPGSDQRTMMALRRHMSGDIDNFPRDVAELVDWFDARDPNPHVRPVPG
                     YGEKIPVWLLGSSLYSAQLAAQLGLPFAFASHFAPDMLFQALHLYRSNFKPSARLEKP
                     YAMVCINIIAADSNRDAEFLFTSMQQAFVKLRRGETGQLPPPIQNMDQFWSPSEQYGV
                     QQALSMSLVGDKAKVRHGLQSILRETDADEIMVNGQIFDHQARLHSFELVMDVKEELL
                     G"
     gene            complement(2097..3341)
                     /gene="mtr"
                     /locus_tag="PN977_003756"
     CDS             complement(2097..3341)
                     /gene="mtr"
                     /locus_tag="PN977_003756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan permease"
                     /protein_id="HDI6043755.1"
                     /translation="MATLTTTQTSPSLLGGVVIIGGTIIGAGMFSLPVVMSGAWFFWS
                     MAALIFTWFCMLHSGLMILEANLNYRIGSSFDTITKDLLGKGWNVVNGISIAFVLYIL
                     TYAYISASGSILHHTFAEMSLNVPARAAGFGFALLVAFVVWLSTKAVSRMTAIVLGAK
                     VITFFLTFGSLLGHVQPTTLFNVAESNASYAPYLLMTLPFCLASFGYHGNVPSLMKYY
                     GKDPKTIVKCLVYGTLMALALYTIWLLATMGNIPRPEFIGIAEKGGNIDVLVQALSGV
                     LNSRSLDLLLVVFSNFAVASSFLGVTLGLFDYLADLFGFDDSAMGRLKTALLTFAPPV
                     VGGLLFPNGFLYAIGYAGLAATIWAAIVPALLARASRKRFGSPKFRVWGGKPMIMLIL
                     VFGVGNALVHILSSFNLLPVYQ"
     gene            complement(3495..5384)
                     /gene="deaD"
                     /locus_tag="PN977_003757"
     CDS             complement(3495..5384)
                     /gene="deaD"
                     /locus_tag="PN977_003757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417631.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DeaD"
                     /protein_id="HDI6043756.1"
                     /translation="MAEFETTFADLGLKAPILEALNDLGYEKPSPIQAECIPHLLNGR
                     DVLGMAQTGSGKTAAFSLPLLQNLDPELKAPQILVLAPTRELAVQVAEAMTDFSKHMR
                     GVNVVALYGGQRYDVQLRALRQGPQIVVGTPGRLLDHLKRGTLDLSKLSGLVLDEADE
                     MLRMGFIEDVETIMAQIPEGHQTALFSATMPEAIRRITRRFMKEPQEVRIQSSVTTRP
                     DISQSYWTVWGMRKNEALVRFLEAEDFDAAIIFVRTKNATLEVAEALERNGYNSAALN
                     GDMNQALREQTLERLKDGRLDILIATDVAARGLDVERISLVVNYDIPMDSESYVHRIG
                     RTGRAGRAGRALLFVENRERRLLRNIERTMKLTIPEVELPNAELLGKRRLEKFAAKVQ
                     QQLESSDLDQYRALLSKIQPTAEGEELDLETLAAALLKMAQGERTLIVPPDAPMRPKR
                     EFRDRDDRGPRDRNDRGPRGDREDRPRRERRDVGDMQLYRIEVGRDDGVEVRHIVGAI
                     ANEGDISSRYIGNIKLFASHSTIELPKGMPGEVLQHFTRTRILNKPMNMQLLGDAQPH
                     TGGERRGGGRGFGGERREGGRNFSGERREGGRGDGRRFSGERREGRAPRRDDSTGRRR
                     FGGDA"
     gene            complement(5377..5457)
                     /gene="yrbN"
                     /locus_tag="PN977_003758"
     CDS             complement(5377..5457)
                     /gene="yrbN"
                     /locus_tag="PN977_003758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YrbN"
                     /protein_id="HDI6043757.1"
                     /translation="MKIADQFHDELCRLAAINFEAHVLHG"
     gene            complement(5564..6448)
                     /gene="nlpI"
                     /locus_tag="PN977_003759"
     CDS             complement(5564..6448)
                     /gene="nlpI"
                     /locus_tag="PN977_003759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020802045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpI"
                     /protein_id="HDI6043758.1"
                     /translation="MKPFLRWCFVATALTLAGCSNTSWRKSEVLAVPLQPTLQQEVIL
                     ARMEQILASRALTDDERAQLLYERGVLYDSLGLRALARNDFSQALAIRPDMPEVFNYL
                     GIYLTQAGNFDAAYEAFDSVLELDPTYNYAHLNRGIALYYGGRDKLAQDDLLAFYQDD
                     PNDPFRSLWLYLAEQKLDEKQAKEVLKQHFEKSDKEQWGWNIVEFYLGNISEQTLMER
                     LKADATDNTSLAEHLSETNFYLGKYYLSLGDLDSATALFKLAVANNVHNFVEHRYALL
                     ELSLLGQDQDDLAESDQQ"
     gene            complement(6557..8692)
                     /gene="pnp"
                     /locus_tag="PN977_003760"
     CDS             complement(6557..8692)
                     /gene="pnp"
                     /locus_tag="PN977_003760"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HDI6043759.1"
                     /translation="MLNPIVRKFQYGQHTVTLETGMMARQATAAVMVSMDDTAVFVTV
                     VGQKKAKPGQDFFPLTVNYQERTYAAGRIPGSFFRREGRPSEGETLIARLIDRPIRPL
                     FPEGFVNEVQVIATVVSVNPQVNPDIVAMIGASAALSLSGIPFNGPIGAARVGYINDQ
                     YVLNPTQDELKESKLDLVVAGTEAAVLMVESEAELLSEDQMLGAVVFGHEQQQVVIQN
                     INELVKEAGKPRWDWQPEPVNEALNARVAALAEARLSDAYRITDKQERYAQVDVIKSE
                     TIATLLAEDETLDENELGEILHAIEKNVVRSRVLAGEPRIDGREKDMIRGLDVRTGVL
                     PRTHGSALFTRGETQALVTATLGTARDAQVLDELMGERTDTFLFHYNFPPYSVGETGM
                     VGSPKRREIGHGRLAKRGVLAVMPDMDKFPYTVRVVSEITESNGSSSMASVCGASLAL
                     MDAGVPIKAAVAGIAMGLVKEGDNYVVLSDILGDEDHLGDMDFKVAGSRDGISALQMD
                     IKIEGITKEIMQVALNQAKGARLHILGVMEQAINAPRGDISEFAPRIHTIKINPDKIK
                     DVIGKGGSVIRALTEETGTTIEIEDDGTVKIAATDGEKAKHAIRRIEEITAEIEVGRV
                     YNGKVTRIVDFGAFVAIGGGKEGLVHISQIADKRVEKVTDYLQMGQEVPVKVLEVDRQ
                     GRIRLSIKEATEQSQPAAAPEAPAAEQGE"
     gene            complement(8939..9208)
                     /gene="rpsO"
                     /locus_tag="PN977_003761"
     CDS             complement(8939..9208)
                     /gene="rpsO"
                     /locus_tag="PN977_003761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HDI6043760.1"
                     /translation="MSLSTEATAKIVSEFGRDANDTGSTEVQVALLTAQINHLQGHFA
                     EHKKDHHSRRGLLRMVSQRRKLLDYLKRKDVARYTQLIERLGLRR"
     gene            complement(9357..10301)
                     /gene="truB"
                     /locus_tag="PN977_003762"
     CDS             complement(9357..10301)
                     /gene="truB"
                     /locus_tag="PN977_003762"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HDI6043761.1"
                     /translation="MSRPRRRGRDINGVLLLDKPQGMSSNDALQKVKRIYNANRAGHT
                     GALDPLATGMLPICLGEATKFSQYLLDSDKRYRVIARLGQRTDTSDADGQIVEERPVT
                     FSAEQLAAALDTFRGDIEQIPSMYSALKYQGKKLYEYARQGIEVPREARPITVYELLF
                     IRHEGNELELEIHCSKGTYIRTIIDDLGEKLGCGAHVIYLRRLAVSKYPVERMVTLEH
                     LRELVEQAEQQDIPAAELLDPLLMPMDSPASDYPVVNLPLTSSVYFKNGNPVRTSGAP
                     LEGLVRVTEGENGKFIGMGEIDDEGRVAPRRLVVEYPA"
     gene            complement(10301..10702)
                     /gene="rbfA"
                     /locus_tag="PN977_003763"
     CDS             complement(10301..10702)
                     /gene="rbfA"
                     /locus_tag="PN977_003763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001712908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HDI6043762.1"
                     /translation="MAKEFGRPQRVAQEMQKEIALILQREIKDPRLGMMTTVSGVEMS
                     RDLAYAKVYVTFLNDKDEDAVKAGIKALQEASGFIRSLLGKAMRLRIVPELTFFYDNS
                     LVEGMRMSNLVTSVVKHDEERRVNPDDSKED"
     gene            complement(10866..13538)
                     /gene="infB"
                     /locus_tag="PN977_003764"
     CDS             complement(10866..13538)
                     /gene="infB"
                     /locus_tag="PN977_003764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HDI6043763.1"
                     /translation="MTDVTIKTLAAERQTSVERLVQQFADAGIRKSADDSVSAQEKQT
                     LIDHLNQKNSGPDKLTLQRKTRSTLNIPGTGGKSKSVQIEVRKKRTFVKRDPQEAERL
                     AAEEQAQREAEEQARREAEESAKREAQQKAEREAAEQAKREAAEQAKREAAEKDKVSN
                     QQDDMTKNAQAEKARREQEAAELKRKAEEEARRKLEEEARRVAEEARRMAEENKWTDN
                     AEPTEDSSDYHVTTSQHARQAEDESDREVEGGRGRGRNAKAARPKKGNKHAESKADRE
                     EARAAVRGGKGGKRKGSSLQQGFQKPAQAVNRDVVIGETITVGELANKMAVKGSQVIK
                     AMMKLGAMATINQVIDQETAQLVAEEMGHKVILRRENELEEAVMSDRDTGAAAEPRAP
                     VVTIMGHVDHGKTSLLDYIRSTKVASGEAGGITQHIGAYHVETENGMITFLDTPGHAA
                     FTSMRARGAQATDIVVLVVAADDGVMPQTIEAIQHAKAAQVPVVVAVNKIDKPEADPD
                     RVKNELSQYGILPEEWGGESQFVHVSAKAGTGIDELLDAILLQAEVLELKAVRKGMAS
                     GAVIESFLDKGRGPVATVLVREGTLHKGDIVLCGFEYGRVRAMRNELGREVLEAGPSI
                     PVEILGLSGVPAAGDEVTVVRDEKKAREVALYRQGKFREVKLARQQKSKLENMFANMT
                     EGEVHEVNIVLKADVQGSVEAISDSLLKLSTDEVKVKIIGSGVGGITETDATLAAASN
                     AILVGFNVRADASARKVIEAESLDLRYYSVIYNLIDEVKAAMSGMLSPELKQQIIGLA
                     EVRDVFKSPKFGAIAGCMVTEGVVKRHNPIRVLRDNVVIYEGELESLRRFKDDVNEVR
                     NGMECGIGVKNYNDVRTGDVIEVFEIIEIQRTIA"
     gene            complement(13563..15050)
                     /gene="nusA"
                     /locus_tag="PN977_003765"
     CDS             complement(13563..15050)
                     /gene="nusA"
                     /locus_tag="PN977_003765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005131833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HDI6043764.1"
                     /translation="MNKEILAVVEAVSNEKALPREKIFEALESALATATKKKYEQEID
                     VRVQIDRKSGDFDTFRRWLVVDEVTQPTKEITLEAARYEDESLNLGDYVEDQIESVTF
                     DRITTQTAKQVIVQKVREAERAMVVDQFREHEGEIITGVVKKVNRDNISLDLGNNAEA
                     VILREDMLPRENFRPGDRVRGVLYSVRPEARGAQLFVTRSKPEMLIELFRIEVPEIGE
                     EVIEIKAAARDPGSRAKIAVKTNDKRIDPVGACVGMRGARVQAVSTELGGERIDIVLW
                     DDNPAQFVINAMAPADVASIVVDEDKHTMDIAVEAGNLAQAIGRNGQNVRLASQLSGW
                     ELNVMTVDDLQAKHQAEAHAAIDTFTKYLDIDEDFATVLVEEGFSTLEELAYVPMKEL
                     LEIEGLDEPTVEALRERAKNALATIAQAQEESLGDNKPADDLLNLEGIDRDLAFKLAA
                     RGVCTLEDLAEQGIDDLADIEGLTDEKAGALIMAARNICWFGDEA"
     gene            complement(15078..15530)
                     /gene="rimP"
                     /locus_tag="PN977_003766"
     CDS             complement(15078..15530)
                     /gene="rimP"
                     /locus_tag="PN977_003766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HDI6043765.1"
                     /translation="MSTLEQKLTEMITAPVEALGFELVGIEFIRGRTSTLRIYIDSED
                     GINVDDCADVSHQVSAVLDVEDPITVAYNLEVSSPGLDRPLFTAEHYARFVGEEVTLV
                     LRMAVQNRRKWQGVIKAVDGEMITVTVEGKDEVFALSNIQKANLVPHF"
BASE COUNT         3635 a         4389 c         3922 g         3789 t
ORIGIN      
        1 tgagaaaagc aatgaaatat tccttagggc cagtgctgtg gtactggcca aaagagacgc
       61 tggaagaatt ttatcagcag gccgccgcca gcagcgccga cgtgatttat cttggtgaag
      121 cggtctgtag caaacgccgg gcaaccaaag ttggcgactg gctggagatg gcaaaatcgc
      181 tcgccggaag tggcaagcag attgtgctct ccacgctggc actggtgcag gcatcctccg
      241 aactgggcga actgaaacgc tatgttgaga acggtgagtt tctgattgaa gccagcgatc
      301 tcggcgtggt gaatatgtgc gctgaacgca aactgccgtt cgtcgccggg cacgcgctga
      361 actgctacaa cgcggtgacg ctgaaaatat tgctcaaaca gggcatgatg cgctggtgta
      421 tgccggtgga gctttcccgc gactggttgg tgaatctgct taatcagtgc gatgagctgg
      481 gcattcgcaa ccagtttgaa gtggaagtcc tgagctacgg tcatctgccg ctggcctact
      541 ccgcccgctg ctttaccgcg cgttcggaag accgcccgaa agatgagtgt gaaacctgct
      601 gcattaagta tccgaacggg cgcaacgtgc tatcgcagga aaaccaacaa gtgtttgtac
      661 tcaatggcat tcagaccatg agcggctacg tttacaacct cggtaacgag ctgacatcca
      721 tgcaggggct ggtcgatgtg gtccgcctgt caccgcaggg tactgacact ttcgcaatgc
      781 tcgacgcctt ccgcgccaat gaaaatggcg ctgcaccact gccgttgacg gcaaacagcg
      841 attgtaacgg ctactggcgg cggctggccg ggctggagct gcaagcgtaa gtatatagct
      901 cactttgtta acaactttaa ctactcttta atgcagtatt aaagattaat cggtaacaaa
      961 gtgagctgtt atgactgata aaaccattgc gttttcgcta ctcgatctgg cccccatccc
     1021 cgaaggctct tcagcgcgag aagcgttctc ccactctctc gatctcgccc gtctggctga
     1081 aaagcacggc tatcatcgct actggctggc agaacaccac aatatgaccg gtattgccag
     1141 tgctgcaacg tcggtgttaa ttggctatct ggcggcgaat accaccacgc tgcatctggg
     1201 gtctggcggc gtgatgttgc ctaaccactc accgttggtg atcgccgaac aattcggcac
     1261 gctcaatacg ctctatccgg ggcgaatcga tttggggctg ggccgcgcgc cgggtagcga
     1321 tcagcgaacc atgatggcgc tgcgtcgtca tatgagcggc gatattgata atttcccccg
     1381 cgatgtcgcg gaactggtgg actggtttga cgcccgcgat cccaatccgc atgtgcgccc
     1441 ggtaccaggc tatggcgaga aaatccccgt gtggttgtta ggctccagcc tttacagcgc
     1501 gcaactggcg gcgcagcttg gtctgccgtt tgcgtttgcc tcacacttcg cgccggatat
     1561 gttgttccag gcgctgcatc tttatcgcag caacttcaaa ccgtcggcac ggttggagaa
     1621 accgtatgcg atggtgtgca tcaatattat cgccgccgac agcaaccgcg atgccgaatt
     1681 cctgtttacc tcaatgcagc aagcctttgt gaagctgcgc cgcggcgaaa ccgggcaact
     1741 gccgccaccg attcaaaata tggatcagtt ctggtcgccg tccgaacagt atggtgtgca
     1801 gcaggcgctg agtatgtcgc tggtgggcga taaagcgaaa gtgcgtcatg gcttgcagtc
     1861 gatcctgcgc gaaaccgacg ccgatgaaat tatggttaac gggcagattt tcgaccacca
     1921 ggcgcgcctg cattcgtttg agctggtgat ggatgtgaag gaagaattat tggggtagtg
     1981 tgttttaacg taggaagcct tatccgagat ggcaacgctg tcctacacaa ttcctgataa
     2041 gcgaagcgca tcaggcattg tgtaggcagc agaaatgtcg gataaggcac cgctgattac
     2101 tgatacaccg gcagcaaatt aaagctcgat aagatatgga ccagtgcgtt gccaacgcca
     2161 aataccagaa tcagcataat catcggcttg ccgccccaga cgcggaattt cgggctacca
     2221 aagcgtttac gcgatgcgcg ggccaacagc gccggaacaa ttgccgccca gatggtagcc
     2281 gctaagccag cataaccaat ggcgtacagg aatccgttag gaaacagcag gccacccaca
     2341 acaggcgggg caaaggtcag caacgctgtt ttcaagcggc ccatagccga gtcatcgaaa
     2401 ccaaacagat ctgccagata gtcaaacaaa cccagcgtaa cgccgaggaa cgaactcgct
     2461 accgcaaagt ttgagaacac aaccagcagc agatccaggc tacggctgtt cagcacgccg
     2521 cttaacgcct gtaccagcac atcaatatta ccgcccttct cggcgatgcc gataaactcc
     2581 ggacgaggga tgttgcccat cgtcgccagc aaccagatgg tatacagcgc cagcgccatc
     2641 agcgtaccgt acaccaggca tttcacgatg gttttcggat ctttgccgta atacttcatc
     2701 aggctcggca cgttaccgtg ataaccaaaa gatgccagac agaatggcag tgtcatcagc
     2761 agatacggcg cataagacgc attgctttcg gcaacgttga acaaggtcgt tggctgcaca
     2821 tgtcccagca ggctaccgaa ggtgaggaaa aacgtaatga ctttcgcccc cagcacaatc
     2881 gccgtcatcc ggctgaccgc tttggtgctc aaccacacca caaacgccac cagtagtgca
     2941 aaaccaaatc cagctgcccg tgcagggacg ttcagcgaca tctccgcgaa ggtgtgatgc
     3001 agaatcgaac cgctggcaga aatataggcg taagtcagga tatagagcac aaaggcaatg
     3061 gaaatgccgt tgactacgtt ccagcctttg ccgagcaggt ctttggtgat ggtgtcaaag
     3121 ctcgaaccga ttcgataatt caggttagct tccagaatca tcaagccgga atgcagcata
     3181 cagaaccagg taaagatcag cgccgccatt gaccagaaga accacgcccc ggacatgacc
     3241 actggcagag aaaacatccc tgcgccaata atggtgccgc cgataatcac cacgccgcca
     3301 agcagcgacg gtgacgtttg ggtggtggtt agtgttgcca tgagggcttc tctccagtga
     3361 aaaatagtgc gactgcgttg ttatgcattg cactgtacca gtacatgagt acaaaagaca
     3421 gaaaaaaagc cccgattatt aaaatcgggg ctgtatatat tattttacag actgtattcg
     3481 ctgttcagcg atgattacgc atcgccaccg aaacgacgac gaccggtaga gtcatcacga
     3541 cgcggtgcgc ggccttcacg acgttcgccg ctaaaacgac gaccatcacc acggccacct
     3601 tcacggcgtt caccgctgaa gttacgaccg ccttcacgac gttcgccacc gaaaccacga
     3661 ccaccgccac gacgctcacc gccagtatgc ggctgtgcat cgcccagtaa ctgcatattc
     3721 atcggcttgt tgagaatgcg agtacgcgta aagtgttgca gcacttcacc cggcatacct
     3781 ttcggcagtt cgatggtgga gtgagaagca aacagcttga tgttaccgat gtaacggctg
     3841 ctgatgtcgc cttcgttagc aatcgcacca acgatatgac gaacttcaac accatcatcg
     3901 cggcccactt caatgcggta cagctgcata tcgccaacat cacgacgttc acgacgcgga
     3961 cgatcttcac ggtcaccacg cgggccacgg tcgttacgat cgcgcggacc acggtcatca
     4021 cggtcacgga attcacgttt cggacgcatc ggcgcatctg gcggaacaat cagagtacgt
     4081 tcaccctgtg ccattttcag cagtgcggcg gccagagttt cgagatccag ctcttcacct
     4141 tctgccgtcg gctgaatttt gctcagcagt gcacggtatt gatccagatc gctgctttcc
     4201 agctgctgct gtactttagc ggcgaatttt tccagacgac gtttgcccag cagttctgcg
     4261 ttcggcagtt caacttccgg aatggtcagc ttcatggtgc gttcaatgtt acgcagcaga
     4321 cgacgctcac ggttctcaac gaacagcagc gcacggccag cacggcccgc acgaccagta
     4381 cgaccgatac ggtgaacgta agactcagaa tccatcggga tatcgtagtt aactaccagg
     4441 ctgatacgct caacgtccag gccacgggcc gcaacgtcgg tcgcaatcag gatgtccaga
     4501 cgaccatctt tcagtcgttc cagtgtctgc tcacgcagcg cctggttcat gtcaccgttc
     4561 agcgcggcgc tgttgtagcc gttacgctca agggcttctg ccacttccag agtcgcgttt
     4621 ttggtacgaa cgaagataat cgccgcatca aaatcttccg cttccaggaa acgtaccagt
     4681 gcttcgtttt tacgcatacc ccagacagtc cagtagctct ggctgatgtc aggacgggta
     4741 gtcacgctgg actgaatgcg cacttcctgc ggctctttca taaagcggcg ggtaatgcga
     4801 cgaatcgctt ccggcatggt tgcagagaac agagcggtct gatgaccttc cgggatctgc
     4861 gccataatag tttcaacgtc ttcgatgaag cccatgcgca gcatttcgtc agcttcatcc
     4921 agaaccagac cgctcagttt agaaaggtcc agagtgccac gtttcaggtg atccagcaga
     4981 cggcccggag taccgacaac gatctgcggc ccctgacgca gggcacgtaa ttgcacgtca
     5041 taacgctggc cgccgtacag ggcaaccacg tttacgccgc gcatgtgttt agagaaatcc
     5101 gtcattgcct cagcaacctg taccgccagt tcgcgggtcg gtgccagcac cagaatctgt
     5161 ggtgctttca gctcaggatc aagattctgc agcagaggta aagagaatgc tgcagttttt
     5221 ccgctccccg tctgggccat acccagaacg tcgcggccat tcagcagatg tggaatacac
     5281 tctgcctgaa ttggagatgg tttttcgtaa cccagatcgt taagggcttc aaggatagga
     5341 gccttcaggc ccagatctgc aaaagtggtt tcgaattcag ccatgtagta cgtgtgcctc
     5401 aaaattaatg gcggccagtc tacataactc atcatgaaat tgatcagcaa ttttcattga
     5461 aaagtgtgaa ccggctcaaa gtaggtgtat taacgaacaa caacgccctc acccgttaag
     5521 gtgatggcaa tcaaaaaaga ttacgggctg atgtgtacgt cagctattgc tggtccgatt
     5581 ctgccaggtc atcttggtcc tggcccagga gcgataattc caacaatgcg tatcggtgct
     5641 caacaaagtt atgaacgttg ttggcaaccg ccagtttgaa cagtgccgtg gcgctgtcca
     5701 aatcccccag acttaggtag tacttaccta aatagaagtt ggtttcactg agatgctcag
     5761 cgagcgaggt gttatccgtt gcgtccgcct tgagcctttc cattaacgtt tgttcgctaa
     5821 tgttgcccag gtagaactcg acaatgttcc atccccactg ttccttatcc gatttttcga
     5881 agtgctgttt caacacttct ttagcctgct tctcatcgag cttctgctcg gcgagataaa
     5941 gccacagact acggaaagga tcattaggat cgtcttgata aaacgccagc agatcatctt
     6001 gcgctaactt gtcacgaccg ccgtaatata atgcgatccc gcgattcaag tgcgcgtagt
     6061 tgtaagttgg atcaagctca agtacagaat caaacgcttc ataggcagca tcaaaattgc
     6121 ctgcctgcgt taaatatatg cctaagtaat tgaatacttc aggcatatcc ggtcggattg
     6181 ccagcgcttg cgaaaaatcg ttacgcgcta atgccctcag accgagacta tcatacaaca
     6241 ctccgcgctc atataaaagc tgtgcgcgtt cgtcatcggt taaagcccga ctggcaagga
     6301 tttgttccat acgtgccaga atcacttcct gctgtaaagt cggttgcaat ggtaccgcga
     6361 ggacttcact tttacgccag gaagtattac tgcatcctgc aagcgtaagt gctgtcgcaa
     6421 cgaaacacca gcgcaaaaaa ggcttcattt cccactcccg aagaccacgg ttgaatgaac
     6481 gtcctgttcc cggttgctaa caaggcgtcc tgcccggtta aaagcccccc gccgcagcgg
     6541 agggcaaatg gcaaccttac tcgccctgtt cagcagccgg agcttccggt gctgcagcag
     6601 gttgagactg ctcagtcgct tctttaatgc tcagacggat acggccctgg cgatcaactt
     6661 ccagaacttt caccggtact tcctgaccca tctgcaggta atcggtcact ttctcaacgc
     6721 gtttgtcagc gatctgagag atgtgtacca gaccttcttt accgccgccg atggcaacaa
     6781 atgcgccaaa gtcaacgata cgggtcactt taccattgta gacgcggccc acttcgattt
     6841 ctgctgtgat ctcttcgata cgacgaatag catgtttcgc tttctcgccg tcggtcgctg
     6901 cgattttcac agtaccgtca tcttcgattt cgatggtggt gccagtttct tcggtcagag
     6961 cacggataac agaaccgcct ttaccgataa cgtctttgat cttgtccggg ttgatcttga
     7021 tggtatggat acgcggagcg aactcagaga tatcgccgcg cggcgcgttg atcgcctgtt
     7081 ccattacgcc caggatatgc agacgcgcac ctttagcttg gttcagtgca acctgcatga
     7141 tctctttggt gataccttca attttgatat ccatctgcaa tgcagagata ccgtcgcggg
     7201 aacccgcaac tttgaagtcc atatcgccca ggtgatcttc gtcgcccaaa atgtcagaca
     7261 gtacaacgta gttgtcgcct tctttcacca gacccattgc gatacccgca acggcagctt
     7321 tgattggcac acctgcgtcc atcagcgcca gagacgcgcc gcacacggaa gccatagaag
     7381 aagaaccgtt ggattcggtg atttcagaca ccacacgtac ggtgtacggg aatttgtcca
     7441 tatccggcat gactgccagc acgccgcgct tcgccagacg accgtgacca atttcacgac
     7501 gcttcggaga accgaccatg ccggtttcgc ctacggagta cggagggaag ttgtagtgga
     7561 acaggaaggt gtcagtacgt tcgcccatca gttcatcaag aacctgcgcg tcacgtgcag
     7621 tacccagcgt tgcggtaacc agcgcctgag tttcaccacg ggtgaacagc gcagaaccgt
     7681 gagtacgcgg cagcacgcca gtacgcacat ccagaccacg aatcatatct ttttcacgac
     7741 cgtcgatacg cggttcgcct gccagtacgc ggctacgaac aacgtttttc tcgatagcgt
     7801 gcagaatttc acccagttcg ttttcgtcca gggtttcatc ttcagcaagc agcgtcgcga
     7861 tggtttcaga tttgatgaca tcaacctgcg cataacgctc ttgtttgtcg gtgatgcggt
     7921 aagcatcgct cagacgagct tcagccagtg cagcaacgcg cgcgttcagc gcttcgttta
     7981 ccggctccgg ctgccagtcc caacgcggtt taccggcttc tttcaccagt tcattgatgt
     8041 tctgaatgac aacctgctgt tgttcatgac caaacactac tgcgcccaac atctggtctt
     8101 cgctcagcag ttcagcttca gattcaacca tcagtacagc ggcttcagta cccgcaacaa
     8161 ccagatccag tttgctctct ttcagctcgt cctgagtcgg gttcagtacg tactggtcat
     8221 tgatgtaacc tacgcgggca gcaccaatcg gaccattgaa cggaatacca gacagagaca
     8281 gtgctgcgga agcaccaatc atcgcgacga tatccgggtt aacttgcggg ttaacagaaa
     8341 ccacggtggc gataacctga acttcgttga cgaagccttc cgggaacagc gggcgaatcg
     8401 ggcggtcaat cagacgcgcg atcagggttt cgccttcgct tgggcggcct tcacgacgga
     8461 agaagctacc cgggatacga ccagcagcgt aggtacgctc ctgatagtta acggtcagtg
     8521 ggaagaagtc ctgacctggt ttggcttttt tctggccaac aacggtaacg aataccgcgg
     8581 tgtcatccat gctaaccata acagcggcag tagcctgacg agccatcatg ccggtttcca
     8641 gagtcacggt gtgttggccg tactggaatt tacgaacgat cggattaagc aatgtaatat
     8701 cctttctctt tcttagacag taccttacgg cactggtgtt aatacccgat cttctgcgca
     8761 tcctcgcgac taatgacaac cctaacccag cccaatgtgg gtaaagcctc tcattagccg
     8821 cgcgaacctc tgcaacggaa gatcattcat agcaacaata cattagtttc cagtgaattg
     8881 ctgccgtcag cttgaaaaaa ggggccactc aggccccctt ttctgaaact cgcaagaatt
     8941 agcgacgcag acccaggcgc tcgatgagct gggtgtaacg tgctacgtct ttacgtttca
     9001 ggtagtcgag cagtttacga cgctgagaaa ccatgcgcag cagaccacga cggctgtggt
     9061 gatctttttt gtgctctgca aagtggccct gcaggtggtt gatctgtgca gtcagcagtg
     9121 ctacctgaac ttcggtagaa ccggtgtcgt ttgcgtcacg accaaactca gaaacgattt
     9181 tagctgttgc ttcagtactt agagacattt taaaactcca aagtatatag aatgaaagga
     9241 cgccgatctc taattcagcg atcccagtgt acgttacgca aagtgttaaa caatttacgc
     9301 gacgttaagc ggcagtattc tactcgtagc gacctgttat cgcaagacgg ttaacattac
     9361 gccgggtatt caaccaccag gcgacgaggc gcgacgcggc cttcatcgtc aatttcgccc
     9421 ataccgataa acttgccgtt ctcaccttct gtaacgcgaa ccagtccttc cagcggcgca
     9481 ccggatgtac gaaccgggtt accatttttg aagtaaacag aagacgttaa cggaagattc
     9541 accaccgggt agtccgaagc tggactgtcc attggcatca gtaatggatc aagtaactcc
     9601 gcggctggaa tatcctgctg ttcggcttgt tcaaccagtt cacgcagatg ttccagggtc
     9661 accatccgtt caaccggata tttacttacc gccagacgac gcaggtaaat aacatgcgcg
     9721 ccacaaccga gtttttcacc caggtcatca atgatggtgc ggatataagt gccttttgag
     9781 cagtgaattt ccagctccag ctcattgcct tcgtggcgaa taaacagcaa ttcataaacg
     9841 gtaatcgggc gcgcttcacg cggaacttca atgccctgac gcgcatattc gtacagtttt
     9901 ttgccctggt atttcagtgc tgaatacatc gaagggatct gttcgatatc gccacggaaa
     9961 gtatccagtg ccgccgccag ttgctctgca ctaaaggtta ccggacgttc ttcaacgatt
    10021 tgtccgtcgg catcagacgt atcggtacgc tgtccaagac gcgcaatgac ccgatagcgt
    10081 ttgtcggagt ccagcagata ctgggaaaac ttcgtcgctt ccccgaggca aatcggcaac
    10141 atgccggtcg ccagcgggtc cagcgcaccg gtatgcccgg cacggttggc gttataaatc
    10201 cgtttcactt tttgcagcgc atcgttgctg gacatcccct gcggtttatc cagcaacaaa
    10261 acgccgttaa tgtcgcgacc gcgacgacga ggacgactca ttagtcctcc ttgctgtcgt
    10321 ccgggttaac acgacgttct tcgtcatgtt tgaccacgct ggtcaccagg tttgacatgc
    10381 gcatcccttc aaccagcgag ttgtcgtaga agaaggtcag ttccggcacg atacgcaggc
    10441 gcatcgcttt ccccagcagg ctgcggatga aaccagaagc ttcctgcaac gctttgatgc
    10501 ccgctttaac cgcgtcttca tctttgtcgt tgaggaacgt cacatatact ttggcatacg
    10561 ccaggtcacg agacatttcg acaccggaaa cggtggtcat catgcccaga cgaggatctt
    10621 taatttcacg ctgcaggatg agagcgatct ctttttgcat ttcctgcgct acgcgctgcg
    10681 ggcgaccaaa ttctttcgcc ataataaatt ctcctgacaa aaaaggggct gttagcccct
    10741 ttttaaaatt aatttcaggt ggaagggctg ttcacgttgg cctgataaga cgcgccagcg
    10801 tcgcatcagg caatccatgc cggatgcggc gtaaacgcct tatcccgcat ggaaccctaa
    10861 aaaccttaag cgatggtacg ttggatttcg atgatttcga atacttcgat cacatcgcca
    10921 gtgcggacgt cgttgtagtt cttaacgccg ataccacatt ccataccgtt acggacttcg
    10981 ttaacgtcat ctttgaagcg gcgcagggac tccagctcgc cttcgtagat aaccacgttg
    11041 tcgcgcagaa cgcggatcgg gttgtgacgt ttaaccacac cttcggtaac catacaacct
    11101 gcgatggcac caaatttcgg tgatttgaat acgtcacgaa cttccgccag accgataatc
    11161 tgttgtttca gttccggaga cagcataccg ctcattgccg ctttcacttc gtcgatcagg
    11221 ttatagatga cggagtagta acgcagatcc aggctttccg cttcaattac tttacgtgca
    11281 gaggcatcag cacgtacgtt gaagccaacc aggatggcgt tggacgccgc agccagggta
    11341 gcgtcggttt cggtgatacc acctacgcca gaaccgatga tcttcacttt aacttcgtca
    11401 gtagacagtt tcagcaagga gtcggagata gcttccacag aaccctgtac gtctgccttc
    11461 aggacgatat tcacttcgtg aacttcgcct tcggtcatgt tggcgaacat gttctcgagt
    11521 ttagatttct gctgacgcgc cagtttaact tcgcggaatt taccctgacg atagagtgca
    11581 acttcacgcg ctttcttctc gtcacgtaca acggtaactt catcacccgc agccggaacg
    11641 ccggacaggc cgaggatttc caccggaatg gacggacccg cttccagcac ttcccgaccc
    11701 agttcgttac gcatagcacg aacacgaccg tattcgaagc cacacagaac gatatcgccc
    11761 ttgtgcagag taccttcacg taccagaacg gtagcaaccg gaccacgacc tttatcgagg
    11821 aaggattcga taaccgcacc gctcgccatg cctttacgta ccgctttcag ttccagaact
    11881 tccgcctgca gcaggatagc gtccagcagc tcatcgatac cagtacccgc tttcgcagac
    11941 acatgtacga actggctttc accgccccac tcttccggca ggatgccgta ctgggagagt
    12001 tcgttcttaa cgcgatccgg atcggcttct ggtttatcga tcttgttcac tgcaacgacc
    12061 accggaacct gcgccgcttt cgcgtgctgg attgcttcga tggtctgcgg cattacgccg
    12121 tcgtcggcag caacaaccag gactacgatg tccgttgcct gcgcaccacg agcacgcatt
    12181 gaagtaaacg cggcgtgccc cggggtgtcc aggaaggtga tcatgccgtt ttcagtttca
    12241 acgtggtatg caccaatgtg ctgggtaatg ccgcccgctt cgccagaggc cactttcgtt
    12301 gaacgaatgt agtccagcag agaggtttta ccgtggtcaa cgtgacccat gatggtcaca
    12361 accggcgcgc gcggttcagc cgctgcaccc gtgtcacggt cgctcattac cgcttcttcc
    12421 agctcgtttt cacgacgcag gataactttg tggcccatct cttcagcaac cagctgtgcg
    12481 gtttcctgat cgataacctg gttgatggtt gccattgcgc ccagtttcat catcgctttg
    12541 atgacctgag agcctttaac cgccatcttg ttcgccagtt cgccaacggt gatggtttca
    12601 ccgatcacaa cgtcacggtt aacggcctga gcaggcttct ggaagccttg ctgcagcgaa
    12661 gaacctttac gttttccgcc tttaccgcca cgtactgctg cgcgtgcttc ttcacgatca
    12721 gcttttgatt cagcgtgttt gttgcctttc ttcggacgcg cagctttcgc gttacgacca
    12781 cggccacggc cgccttcgac ttcacgatcg ctttcgtctt ctgcctggcg agcatgttga
    12841 gaagtagtga cgtgataatc gctggaatct tcagtcggtt ccgcgttatc agtccatttg
    12901 ttttcttccg ccatacgacg tgcttcttca gcaacgcgac gtgcttcttc ttcgagttta
    12961 cgacgcgctt cttcttcggc tttacgcttg agctctgcag cttcctgctc acggcgggct
    13021 ttttcagcct gggcgttttt agtcatatcg tcttgttgat tgctcacttt gtctttttcc
    13081 gcagcttcac gtttcgcttg ttcagcagct tcacgcttag cttgttctgc ggcctcacgt
    13141 tcagcttttt gttgcgcctc acgtttggcc gattcttctg cctcacgacg ggcttgctct
    13201 tccgcttcac gctgcgcttg ctcttccgca gcaaggcgtt cagcctcttg cggatcgcgt
    13261 ttcacaaagg tgcgtttctt gcggacttcg atttgtaccg atttgctttt tccaccggta
    13321 ccaggaatgt taagggtgct gcgtgtttta cgttgcagcg tcaatttgtc cgggcctgaa
    13381 tttttctgat tcaggtggtc aatcaaagtc tgtttctctt gtgcagacac agagtcgtca
    13441 gcagacttcc ggatacctgc atcagcaaat tgctgtacca ggcgttccac ggaggtctgt
    13501 cgctctgcgg ccagcgtttt aatcgttaca tctgtcatgc tgttccttcc tgctacagtt
    13561 tattacgctt cgtcaccgaa ccagcaaata ttacgggcag ccataatcag tgctccggct
    13621 ttttcgtcgg tcaacccttc gatatcagcc agatcatcaa tgccctgttc ggcgagatct
    13681 tccagcgtac aaacgccacg ggcggccagt ttgaatgcca aatcacgatc tatcccttca
    13741 aggttcagca gatcgtcagc cggtttgtta tcaccgaggc tttcttcctg ggcctgtgca
    13801 atggtggcca gtgcattttt agcacgctcg cgcagtgctt caacggtcgg ctcatcaagg
    13861 ccttcgattt ccaacagctc tttcatcggc acataggcca gttcttccag cgtcgagaag
    13921 ccttcttcta ccagaacagt cgcgaagtct tcatcgatgt cgagatattt ggtgaaggtg
    13981 tcgatcgctg cgtgcgcttc cgcctgatgc ttagcctgca ggtcgtcaac ggtcataacg
    14041 ttgagttccc aaccgctcag ctgcgaagcc agacgcacgt tctgaccgtt acggccaatc
    14101 gcctgcgcca ggttaccggc ttcgacggcg atatccatgg tgtgtttatc ttcatccacc
    14161 acgatagaag caacgtctgc cggtgccatt gcgttaatca cgaactgcgc cgggttatca
    14221 tcccacagga cgatatcgat acgctcgccg cccagttcag tagaaaccgc ctgaacacgc
    14281 gcgccacgca tacctacgca agcacctacc ggatcgatac gtttatcgtt ggttttcacc
    14341 gcgattttcg cacgagaacc cggatcgcga gccgctgctt taatttcaat cacttcttcg
    14401 ccgatttccg gcacttcaat gcggaacagt tcgatcagca tttccggctt ggaacgggtg
    14461 acgaacagtt gcgcgccacg cgcttccggg cgaacggaat agagcacgcc acgaacgcgg
    14521 tcgccaggac ggaagttttc acgcggcagc atatcttcgc gcaggatcac ggcttcagcg
    14581 ttgttgccca gatccagaga gatgttgtcg cggtttactt ttttcaccac gccggtgatg
    14641 atttcacctt cgtgttcacg gaactgatca accaccatcg cacgttcggc ttcacgcact
    14701 ttctgcacga taacctgttt tgccgtctgg gtagtgatac ggtcaaaggt aacagactca
    14761 atctgatctt caacgtaatc gcccaggttc aggctttcat cttcataacg tgcggcttca
    14821 agggtgattt ccttggtcgg ctgggtgact tcatcaacaa ctaaccagcg acggaaggtg
    14881 tcaaaatcac cgcttttgcg atcgatctgt acgcggacgt cgatctcttg ttcatatttt
    14941 ttctttgttg ctgtcgccag cgcgctttcc aatgcttcga aaatcttctc gcgaggtagc
    15001 gccttttcat tggatacggc ttcaactaca gccaaaattt ctttgttcat cgcgggcttt
    15061 tcacctcatc cagactatta aaagtgggga accaggttcg ccttctggat attactcagc
    15121 gcgaacactt catctttacc ttcgacggta actgtgatca tttcaccgtc taccgctttg
    15181 ataacgccct gccatttacg acggttttgt accgccatgc ggagaaccag agtcacctct
    15241 tctccgacga aacgggcgta gtgttcagcc gtgaacagtg ggcgatcgag acccggtgag
    15301 gagacttcca ggttataagc aacggtgatg ggatcttcaa catccagcac agcacttacc
    15361 tggtggctca catcagcaca atcatcaaca ttgatgccat cttcactatc aatatagatg
    15421 cgcagtgtgg atgtgcgacc gcgaataaat tcgatgccaa ccagttcaaa acccagggcc
    15481 tcaactggcg cagtaatcat ctctgttaat ttttgctcta atgtggacaa gcccaccccc
    15541 aagacataaa aaaagggcct aaagcccagt tattctgtag tcagataaca aaaaaccccg
    15601 ataaatcggg gctttatata actgaaccct ataaccgcaa ctgcggtctg gagcactttc
    15661 cagaaggatt ttttcaaatc ccactacgaa ggccgaagtc ttcacagtat atttgaaaaa
    15721 ggactctaag ggaaa
//
LOCUS       DAMZFT010000116        15510 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.116, whole genome shotgun sequence.
ACCESSION   DAMZFT010000116 DAMZFT010000000
VERSION     DAMZFT010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15510)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15510)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15510
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.116"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(<1..790)
                     /locus_tag="PN977_003767"
     CDS             complement(<1..790)
                     /locus_tag="PN977_003767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE9 sensor domain-containing protein"
                     /protein_id="HDI6043766.1"
                     /translation="MLSLYEKIKIRLIILFLLAALSFIGLFFIINYQLVSERAVKRAD
                     SRFELIQKNVGYFFKDIERSALTLKDSLYLLKNTEEIQRAVILKMEMMPFLDSVGLVL
                     DDNKYYLFSRRTNDKIVVYHQEQVNGPLVDESGRVIFADFNPSKRPWSVASDDSNNSW
                     YPAYNCFDRPGKKCISFTLRINGKDHDLLAVDKIHVDLNWRYLNEYLDHISANDEVLF
                     LKQGHEIIAKNQLAREKLIIYNSEGNYNIIDSVDTEYIEKTSVVP"
     gene            complement(967..1878)
                     /gene="gsiD"
                     /locus_tag="PN977_003768"
     CDS             complement(967..1878)
                     /gene="gsiD"
                     /locus_tag="PN977_003768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiD"
                     /protein_id="HDI6043767.1"
                     /translation="MRLFNWRRQAVLNAMPLVKPDQVRTPWYEFWRRFRRQHMAMTAA
                     LFVILLIVVAIFARWIAPYDAENYFDYDNLNNGPSLQHWFGVDSLGRDIFSRVLVGAQ
                     ISLAAGVFAVFIGAAIGTLLGLLAGYYEGWWDRLIMRICDVLFAFPGILLAIAVVAVL
                     GSGIANVIIAVAIFSIPAFARLVRGNTLVLKQQTFIESARSIGASDMTILLRHILPGT
                     VSSIVVFFTMRIGTSIISAASLSFLGLGAQPPTPEWGAMLNEARADMVIAPHVAVFPA
                     LAIFLTVLAFNLLGDGLRDALDPKIKG"
     gene            complement(1881..2801)
                     /gene="gsiC"
                     /locus_tag="PN977_003769"
     CDS             complement(1881..2801)
                     /gene="gsiC"
                     /locus_tag="PN977_003769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiC"
                     /protein_id="HDI6043768.1"
                     /translation="MLNYVIKRLLGLIPTLFIVSVLVFLFVHMLPGDPARLIAGPEAD
                     AQVIELVRQQLGLDQPLYHQFWHYISNAVQGDFGLSMVSRRPVADEIASRFMPTLWLT
                     ITSMVWAVIFGMAAGIIAAVWRNRWPDRLSMTIAVSGISFPAFALGMLLIQVFSVELG
                     WLPTVGADSWQHYILPSLTLGAAVAAVMARFTLASFVDVLSEDYMRTARAKGVSETWV
                     VLKHGLRNAMIPVVTMMGLQFGFLLGGSIVVEKVFNWPGLGRLLVDSVEMRDYPVIQA
                     EILLFSLEFILINLVVDVLYAAINPAIRYK"
     gene            complement(2819..4357)
                     /gene="gsiB"
                     /locus_tag="PN977_003770"
     CDS             complement(2819..4357)
                     /gene="gsiB"
                     /locus_tag="PN977_003770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter substrate-binding
                     protein GsiB"
                     /protein_id="HDI6043769.1"
                     /translation="MARAVHRSGLVALGIATALMASCAFAAKDVVVAVGSNFTTLDPY
                     DANDTLSQAVAKSFYQGLFGLDKEMKLKNVLAESYTVSDDGLTYTVKLREGIKFQDGT
                     DFNAAAVKANLDRASDPANHLKRYNLYKNIAKTEAIDPTTVKITLKQPFSAFINILAH
                     PATAMISPAALEKYGKDIGFHPVGTGPYELDTWNQTDFVKVKKFAGYWQPGLPKLDSI
                     TWRPVADNNTRAAMLQTGEAQFAFPIPYEQAALLEKNKNIELMASPSIMQRYISMNVT
                     QKPFDNPKVREALNYAINRPALVKVAFAGYATPATGVVPPSIAYAQSYKPWPYDPVKA
                     RELLKEAGYPNGFSTTLWSSHNHSTAQKVLQFTQQQLAQVGIKAQVTAMDAGQRAAEV
                     EGKGQKESGVRMFYTGWSASTGEADWALSPLFASQNWPPTLFNTAFYSNKQVDDFLAQ
                     ALKTNDPAEKTRLYKAAQDIIWQESPWIPLVVEKLVSAHSKNLTGFWIMPDTGFSFED
                     ADLQ"
     gene            complement(4377..6248)
                     /gene="gsiA"
                     /locus_tag="PN977_003771"
     CDS             complement(4377..6248)
                     /gene="gsiA"
                     /locus_tag="PN977_003771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter ATP-binding protein
                     GsiA"
                     /protein_id="HDI6043770.1"
                     /translation="MPHSDELDAGDVLAVENLNIAFMQDQQKIAAVRNLSFSLQRGET
                     LAIVGESGSGKSVTALALMRLLEQAGGLVQCDKMLLRRRSREVIELSEQSAAQMRHVR
                     GADMAMIFQEPMTSLNPVFTVGEQIAESIRLHQNASREEAMVEAKRMLDQVRIPEAQT
                     ILSRYPHQLSGGMRQRVMIAMALSCCPAVLIADEPTTALDVTIQAQILQLIKVLQKEM
                     SMGVIFITHDMGVVAEIADRVLVMYQGEAVETGSVEQIFHAPQHPYTRALLAAVPQLG
                     AMKGLDYPRRFPLISLEHPAKQEPPIEQKTVVDGEPVLRVRNLVTRFPLRSGLLNRVT
                     REVHAVEKVSFDLWPGETLSLVGESGSGKSTTGRALLRLVESQGGEIIFNGQRIDTLS
                     SGKLQALRRDIQFIFQDPYASLDPRQTIGDSIIEPLRVHGLLPGKEAAARVAWLLERV
                     GLLPEHAWRYPHEFSGGQRQRICIARALALNPKVIIADEAVSALDVSIRGQIINLLLD
                     LQRDFGIAYLFISHDMAVVERISHRVAVMYLGQIVEIGPRRAVFENPQHPYTRKLLAA
                     VPVAEPSRQRPQRVLLSDDLPSNIHLRGEEVAAVSLQCVGPGHYVAQPQSEYAFMRR"
     gene            complement(6235..7200)
                     /gene="iaaA"
                     /locus_tag="PN977_003772"
     CDS             complement(6235..7200)
                     /gene="iaaA"
                     /locus_tag="PN977_003772"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HDI6043771.1"
                     /translation="MGKAVIAIHGGAGAISRAQMSLQQELRYIEALSAIVETGQKMLE
                     AGESALDVVTEAVRLLEECPLFNAGIGAVFTRDETHELDACVMDGNTLKAGAVAGVSH
                     LRNPVLAARLVMEQSPHVMMIGEGAENFAFAHGMERVSPEIFSTPLRYEQLLAAREEG
                     ATVLDHSGAPLDEKQKMGTVGAVALDLDGNLAAATSTGGMTNKLPGRVGDSPLVGAGC
                     YANNASVAVSCTGTGEVFIRALAAYDIAALMDYGGLSLAEACERVVMEKLPALGGSGG
                     LIAIDHEGNVALPFNTEGMYRAWGYAGDTPTTGIYREKGDTVATQ"
     gene            7404..8639
                     /gene="moeA"
                     /locus_tag="PN977_003773"
     CDS             7404..8639
                     /gene="moeA"
                     /locus_tag="PN977_003773"
                     /EC_number="2.10.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HDI6043772.1"
                     /translation="MEFTTGLMSLDTALNEMLSRVTPLTAQETLPLVQCFGRILASDV
                     VSPLDVPGFDNSAMDGYAVRLADIASGQPLPVAGKSFAGQPYHGEWPAGTCIRIMTGA
                     PVPEGCEGVVMQEQTEQTDNGVRFTAEVRSGQNIRRRGEDISAGAVVFPAGTRLTTAE
                     LPVIASLGIAEVPVIRKVRVALFSTGDELQLPGQPLGDGQIYDTNRLAVHLMLEQLGC
                     EVINLGIIRDDPHALRAAFIEADSQADVVISSGGVSVGEADYTKTILEELGEIAFWKL
                     AIKPGKPFAFGKLSNSWFCGLPGNPVSATLTFYQLVQPLLAKLSGNTASGLPARQRVR
                     TASPLKKSPGRLDFQRGVLQRNADGELEVTTTGHQGSHIFSSFSLGNCFIVLERDRGN
                     VEVGEWVEVEPFNALFGGL"
     gene            8639..9388
                     /gene="moeB"
                     /locus_tag="PN977_003774"
     CDS             8639..9388
                     /gene="moeB"
                     /locus_tag="PN977_003774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HDI6043773.1"
                     /translation="MAELSDQEMLRYNRQIILRGFDFDGQEALKDSRVLVVGLGGLGC
                     AASQYLASAGVGNLTLLDFDTVSLSNLQRQTLHSDATVGQPKVESARDALTRINPYIA
                     ITPVNALLDDAELAAMIAKHDLVLDCTDNVAVRNQLNAGCFAAKVPLVSGAAIRMEGQ
                     ITVFTYQDGEPCYRCLSRLFGENALTCVEAGVMAPLIGVIGSLQAMEAIKLLAGYGKP
                     ASGKIVMYDAITCQFREMKLMRNPGCEVCGQ"
     gene            complement(9464..10126)
                     /gene="fsa"
                     /locus_tag="PN977_003775"
     CDS             complement(9464..10126)
                     /gene="fsa"
                     /locus_tag="PN977_003775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415346.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HDI6043774.1"
                     /translation="MELYLDTSDVVAVKALSRIFPLAGVTTNPSIIAAGKKPLEVVLP
                     QLHEAMGGQGRLFAQVMATTAEGMVNDALKLRSIIADIVVKVPVTAEGLAAIKMLKAE
                     GIPTLGTAVYGAAQGLMSALAGAEYVAPYVNRIDAQGGSGIQTVTDLHQLLKMHAPQA
                     KVLAASFKTPRQALDCLLAGCESITLPLDVAQQMISYPAVDAAVAKFEQDWLGAFGRT
                     SI"
     gene            10257..11156
                     /locus_tag="PN977_003776"
     CDS             10257..11156
                     /locus_tag="PN977_003776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HDI6043775.1"
                     /translation="MIFNIQRYSTHDGPGIRTVVFLKGCSLGCRWCQNPESRARTQDL
                     LYDARLCLEGCELCAKAAPDVIERSLNGLLIHREKLTPEHLTALTDCCPTQALTVCGE
                     VKSVEEIMATVLRDKPFYDRSGGGLTLSGGEPFMQPEMATELLQASHEAGIHTAVETC
                     LHVPWKYISPSLPYIDLFLADLKHVADAPFKQWTDGNAARVLDNLKKLAAAGKKIIIR
                     VPLIQGFNADETAVKAITDFAADELHVGEIHFLPYHTLGINKYHLLNLPYDAPEKPLD
                     APELLDFAQQYACQKGLTATLRG"
     gene            11162..13594
                     /locus_tag="PN977_003777"
     CDS             11162..13594
                     /locus_tag="PN977_003777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HDI6043776.1"
                     /translation="MTTLKLDTLSDRIKAHKNALVHIVKPPVCTERAQHYTEMYQQHL
                     DKPIPVRRALALAHHLANRTIWIKHDELIIGNQASEVRAAPIFPEYTVSWIEKEIDDL
                     ADRPGAGFAVSEENKRVLHEVCPWWRGQTVQDRCYGMFTDEQKGLLATGIIKAEGNMT
                     SGDAHLAVNFPLLLEKGLDGLREKVAERRSRINLTVLEDLHGEQFLKAIDIVLVAVSE
                     HIERFAALAREMAATETRESRRDELLTIAENCDLIAHQPPQTFWQALQLCYFIQLILQ
                     IESNGHSVSFGRMDQYLYPYYRRDVELNQTLDREHAIEMLHSCWLKLLEVNKIRSGSH
                     SKASAGSPLYQNVTIGGQNLVDGQPMDAVNPLSYALLESCGRLRSTQPNLSVRYHAGM
                     SNDFLDACVQVIRCGFGMPAFNNDEIVIPEFIKLGIEPQDAYDYAAIGCIETAVGGKW
                     GYRCTGMSFINFARVMLAALEGGRDATSGKVFLPQEKALSAGSFNNFDEVMDAWDTQI
                     RYYTRKSIEIEYVVDTMLEENVHDILCSALVDDCIERAKSIKQGGAKYDWVSGLQVGI
                     ANLGNSLAAVKKLVFEQGAIGQQQLAAALADDFDGLTHEQLRQRLINGAPKYGNDDDT
                     VDTLLARAYQTYIDELKQYHNPRYGRGPVGGNYYAGTSSISANVPFGAQTMATPDGRK
                     AHTPLAEGASPASGTDHLGPTAVIGSVGKLPTAAILGGVLLNQKLNPATLENESDKQK
                     LMILLRTFFEVHKGWHIQYNIVSRETLLEAKKHPDQYRDLVVRVAGYSAFFTALSPDA
                     QDDIIARTEHML"
     gene            13740..14555
                     /gene="ybiV"
                     /locus_tag="PN977_003778"
     CDS             13740..14555
                     /gene="ybiV"
                     /locus_tag="PN977_003778"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase YbiV"
                     /protein_id="HDI6043777.1"
                     /translation="MSVKVIVTDMDGTFLNDAKTYNQPRFMAQYQELKKRGIEFVVAS
                     GNQYYQLISFFPELKDEISFVAENGALVYEHGKQLFHGELTRHESRIVIGELLKDKQL
                     NFVACGLQSAYVSENAPEAFVALMAKHYHRLKPVKDYQEIDDVLFKFSLNLPDEQIPL
                     VIDKLHIALDGIMKPVTSGFGFIDLIIPGLHKANGISRLLKRWDLSPQNVVAIGDSGN
                     DAEMLKMARYSFAMGNAAENIKQIARYATDDNNHEGALNVIQAVLDNTSPFNS"
     gene            14707..>15510
                     /locus_tag="PN977_003779"
     CDS             14707..>15510
                     /locus_tag="PN977_003779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1479 family protein"
                     /protein_id="HDI6043778.1"
                     /translation="MASTFTSDTLPADHKAAIRQMKHALRAQLGDVQQIFNQLSDDIA
                     TRVAEINALKAQGDAVWPVLSYADIKAGHVTAEQREQIKRRGCAVIKGHFPREQALGW
                     DQSMLDYLDRNRFDEVYKGPGDNFFGTLSASRPEIYPIYWSQAQMQARQSEEMANAQS
                     FLNRLWTFESDGKQWFNPDVSVIYPDRIRRRPPGTASKGLGAHTDSGALERWLLPAYQ
                     RVFANVFNGNLAQYDPWHAAHRTEVEEYTVDNTTKCSVFRTFQGWTALSD"
BASE COUNT         3790 a         4275 c         3885 g         3560 t
ORIGIN      
        1 ttggcaccac tgatgttttt tcgatatatt cagtatcgac agaatcaata atattataat
       61 taccttcgct attataaata atcagttttt cacgcgcgag ttgattcttg gcaatgatct
      121 catggccttg tttcaaaaat agaacttcat cattagcgct gatgtgatca agatactcgt
      181 tcagatatcg ccagtttaaa tcgacatgta ttttatccac cgctaacaaa tcgtgatctt
      241 tgccgttgat gcgtagcgta aaagagatac attttttacc cggacgatca aagcaattgt
      301 atgccggata ccagctgttg ttagagtcat ctgaagccac cgaccacggt cgtttcgatg
      361 ggttaaaatc ggcaaaaata acccgccctg actcgtcgac aagcggtcca tttacttgtt
      421 cctgatgata aacaacgatt ttatcattcg tcctccgcga aaagagataa tatttattat
      481 catcaagtac cagtcccacc gagtctaaaa atggcatcat ttccattttc agaattacgg
      541 cgcgttgaat ctcctctgta ttttttaata aatatagtga gtcctttaat gtcagggccg
      601 aacgttcaat atctttaaag aaatagccaa cgtttttctg aataagttca aagcggctat
      661 cggcacgttt taccgcgcgt tccgatacca gttgatagtt aatgatgaaa aaaagaccaa
      721 taaatgacag tgctgccaat aaaaataaaa ttatcagcct tatctttatc ttttcgtata
      781 aactcagcat gttaatacat ttaacaaaat aactgtctga acgctgtgcc gctgcaaaat
      841 agtcccttgc tgcggctttt taaccaaatt acccgattct acaaaatcag cagaaagtta
      901 caacacttgc ttagggcaat aatttcctgt gaggtgatta ccctttcaag caatattcaa
      961 acgtaactat cctttaattt tcggatccag cgcatcgcgt aaaccatcgc ccaacaaatt
     1021 gaacgccagt acggtcagaa aaatagccag ggccggaaaa acagcgacat gcggcgcgat
     1081 aaccatatcc gctcgtgcct cattgagcat tgctccccac tctggtgtcg gcggctgcgc
     1141 accgaggccg agaaatgaca ggctggcggc agagataatc gaggtaccaa tgcgcatggt
     1201 gaaaaacacc acgatagaag agacggtccc aggcaggata tgacgcaata aaatggtcat
     1261 atcgctggca ccaatactgc gtgctgactc gataaaggtt tgctgtttca acaccagcgt
     1321 gttgccgcgc accagccggg caaacgcggg gatggaaaaa atggcgactg cgataatcac
     1381 gttagcaatg ccgcttccca acaccgcaac aacagcgatc gccagtaaaa tacccgggaa
     1441 ggcaaacagc acatcgcaaa tacgcatgat cagccgatcc caccagcctt cataatatcc
     1501 agccagcaag cccagcaacg tcccgatcgc cgcaccgata aacacggcaa acacgcccgc
     1561 cgccagcgag atttgcgcac caaccaggac acggctgaaa atgtcgcgcc ccagtgaatc
     1621 gacgccaaac cagtgctgca aagaaggtcc gttattcaga ttgtcataat caaaataatt
     1681 ttcggcgtca tagggagcga tccagcgtgc aaaaatggcc accacaatca ataaaataac
     1741 gaataatgcg gcggtcatcg ccatatgctg acggcgaaat cgtcgccaga attcatacca
     1801 cggtgtacgc acctggtcag gtttgaccag tggcatggcg tttaacaccg cctgacgtcg
     1861 ccagttaaat agtcgcatcc ttacttgtac ctgatagccg ggttaatggc ggcgtaaagc
     1921 acatccacca ctaagttgat aagaataaat tccagcgaga aaagcagaat ttccgcctga
     1981 atcaccgggt aatcgcgcat ttctacggag tcaacgagta agcgtccaag tccgggccag
     2041 ttgaaaactt tctccaccac gatagaacca ccgagcaaaa agccaaactg taagcccatc
     2101 atggtcacta ccgggatcat cgcgttacgt agcccgtgtt tgaggacaac ccaggtttcg
     2161 ctcacccctt tcgccctcgc ggtacgcata taatcttcgc ttaaaacatc gacaaacgac
     2221 gcgagggtaa agcgcgccat cacggctgcc actgccgcgc cgagcgtcag ggagggtaaa
     2281 atgtagtgct gccaactgtc tgctcccacg gtaggcagcc agcccagttc aacggagaat
     2341 acctgaatta aaagcatccc cagagcaaat gccggaaacg agatccccga caccgcaatg
     2401 gtcatactca atcgatccgg ccaacggtta cgccagacgg cggcgataat tcccgccgcc
     2461 ataccaaata tcaccgccca gaccatactg gttatggtca gccacagcgt tggcataaag
     2521 cggctggcaa tctcatcagc aaccggacga cgcgacacca tcgacaggcc aaaatctccc
     2581 tgcacagcat tgctgatata gtgccagaac tggtgataca gcggctgatc caaccccagc
     2641 tgctgacgca ccagttctat aacctgcgca tcagcttcgg gcccggcaat taatcgcgcc
     2701 ggatcgccgg gcagcatatg gacaaataaa aacaccagca ccgagacgat aaacagcgtc
     2761 ggaatcaacc ccagcaagcg tttgataacg taattaagca ttccactccc tgcgttgctt
     2821 attgcaaatc tgcgtcttca aagctgaagc cggtgtctgg catgatccaa aaaccggtca
     2881 ggtttttact gtgtgccgac accagttttt ctaccaccag cgggatccac ggcgactctt
     2941 gccagatgat atcctgcgcc gccttatata aacgggtctt ttccgccgga tcattagttt
     3001 ttagtgcctg agccaggaag tcatccacct gtttattgct gtaaaacgcg gtattaaaca
     3061 gcgttggtgg ccagttttgc gaggcaaaca gcggcgatag tgcccagtca gcttcgccgg
     3121 ttgaagccga ccagccagtg tagaacatcc gcacgccgct ctctttttgc cctttacctt
     3181 cgacttctgc cgcccgctgt ccggcatcca tcgcagtcac ctgggcttta atcccgacct
     3241 gcgctaactg ctgctgggta aattgcagca ctttctgcgc ggtgctgtgg ttatgtgacg
     3301 accacagcgt ggtactgaaa ccgttgggat atcccgcctc tttcagtaat tcgcgcgctt
     3361 tcactggatc gtaaggccac ggtttataac tttgcgcata ggcgatactc ggcggtacca
     3421 caccagtagc tggcgttgca tagcccgcga aggccacttt caccagcgcc gggcggttaa
     3481 tggcgtaatt cagcgcctca cggaccttcg ggttatcgaa cggcttttgc gtcacgttca
     3541 tactgatata acgctgcata attgacggac tggccatcaa ctcgatattt ttgtttttct
     3601 ccagcaatgc ggcctgctcg taaggaatgg ggaaagcaaa ctgcgcttca ccggtttgta
     3661 gcattgccgc gcgggtgttg ttatccgcaa ccggacgcca ggttatgctg tccagtttgg
     3721 gcaatcctgg ctgccagtaa cccgcgaatt ttttcacctt cacaaaatcg gtctgattcc
     3781 aggtatccag ttcatacggc ccggttccca ccggatgaaa accaatatcc ttgccatatt
     3841 tttccagcgc tgccggcgaa atcatcgcgg tcgccggatg ggcaagaata ttaataaacg
     3901 ctgagaacgg ctgtttgagg gtaatcttta ccgttgttgg atcgatcgct tccgttttgg
     3961 caatattctt atacaggttg tagcgtttaa gatgattcgc cggatcgctg gcccggtcca
     4021 gattcgcttt taccgccgct gcgttgaagt cggttccatc ctggaattta attccttccc
     4081 gcagtttcac ggtgtaagta aggccgtcat cggaaacggt ataactctcc gccagcacgt
     4141 ttttcagttt catctcttta tccagaccga acagcccctg gtaaaacgat ttcgctacgg
     4201 cctgagataa cgtgtcattc gcgtcatatg gatcgagcgt ggtgaagttc gatcctaccg
     4261 ccaccaccac atctttggca gcgaatgcac aagatgccat caacgctgtc gcaatgccca
     4321 gcgccactaa cccactacgg tgtacagctc ttgccatttt attctccgcc tgaatgttat
     4381 ctacgcatga atgcgtattc tgattgtggt tgtgcgacgt aatgccccgg cccgacgcat
     4441 tgcaacgaga cggctgcaac ctcttcgcca cgcagatgaa tattgctggg aagatcgtcc
     4501 gacagcagta cacgctgcgg tcgttgtcgg gacggttcag cgaccggaac tgccgccagt
     4561 aatttacgcg tataaggatg ctgcgggttt tcgaagaccg cgcgccgtgg accaatttca
     4621 acaatttgcc cgagatacat caccgccaca cgatgactaa tccgctctac cacggccata
     4681 tcgtgggaga taaacagata cgcaatgccg aaatcacgct ggagatcgag caacaagttg
     4741 ataatctgcc cgcgaataga aacatccagc gccgagacgg cttcgtcggc aatgatcact
     4801 tttggattca atgccaacgc gcgagcaatg cagatgcgct ggcgctgacc gccggaaaac
     4861 tcatgcgggt aacgccaggc atgttcaggt aacaggccca cgcgctccag caaccacgca
     4921 acacgtgcag ccgcttcttt acctggcaat aaaccgtgta cgcgcagcgg ttcgataatc
     4981 gaatcaccga tggtctgacg tgggtccagc gaagcgtaag ggtcctgaaa aataaactga
     5041 atatcgcggc gcaatgcctg aagtttaccg gatgacaagg tatcgattcg ctgaccgtta
     5101 aagataattt cgccgccctg cgattcgacc aggcgcagca acgcccgccc ggtagtggat
     5161 ttaccgctgc cagactcgcc caccagcgat agcgtttcac caggccagag atcaaaactg
     5221 actttctcaa cggcatgcac ttcccgcgtt acgcgattca acaaaccgct gcgcaaaggg
     5281 aaacgggtga ccagattacg cacccgtaaa acaggttcgc catccaccac cgttttctgc
     5341 tcgatgggtg gctcctgttt cgctggatgt tcaagcgata tcaacgggaa acgtcgggga
     5401 taatctaacc ctttcatcgc accaagttgc ggaacagcag ctaacagcgc acgggtataa
     5461 ggatgttgcg gtgcatgaaa aatctgttcg acgctacccg tttccaccgc ctcgccctga
     5521 tacatcacca gaacccgatc ggcaatctct gccaccacgc ccatatcgtg agtgataaag
     5581 ataacgccca tcgacatctc tttttgcaat actttgatta attgcaggat ctgcgcctga
     5641 atagtgacat ccagcgcggt ggttggctca tcggcaatca gcaccgccgg gcagcatgac
     5701 agcgccatcg caatcatcac tcgctggcgc atcccgccag agagttgatg cggataacgt
     5761 gaaagaatgg tttgtgcctc cggaatgcgt acctgatcca gcatccgctt cgcctcgacc
     5821 atcgcttctt cacgactggc gttctgatgc agacgaattg attcggcaat ctgttcaccc
     5881 acagtaaata ccgggttcag cgatgtcatc ggttcctgaa atatcatcgc catatccgca
     5941 ccgcgcacat ggcgcatttg tgcagcgctc tgctcgctaa gttcaatcac ttcgcgactg
     6001 cgccgccgca acagcatttt atcgcactgt actaaaccgc ccgcctgttc caacagacgc
     6061 atcaatgcca gcgcagtcac tgacttaccg gagccggatt cgccaacaat tgccagcgtc
     6121 tcaccgcgtt gcaggctaaa agagagattg cggaccgcag ctattttctg ctggtcctgc
     6181 ataaaggcaa tattcagatt ttcaaccgcc agcacatcac cggcatcaag ttcatcactg
     6241 tgtggcaacg gtgtcccctt tttcacggta gataccggtg gttggcgtat cgcctgcgta
     6301 gccccaggcg cgatacattc cttcggtgtt aaacggtagc gcgacgttac cttcatggtc
     6361 gatagcgatt aagccaccgc taccgccaag cgcagggagt ttttccatta ctacccgctc
     6421 gcaggcttcc gcgagactta atccgccgta atccattaac gcggcgatgt catatgccgc
     6481 cagcgcgcgg atgaagactt cgcccgtgcc ggtacaagaa accgccacac tggcgttatt
     6541 ggcgtagcat ccggcaccca ctaagggact atcgccaact cgtccgggta atttattggt
     6601 cattccgccc gtggacgtgg ctgccgccag attgccgtct aaatccaacg ccacggcccc
     6661 cacggtgccc attttctgtt tttcatccag tggcgcaccg ctatggtcga ggactgttgc
     6721 cccttcctcg cgcgctgcca gtagttgttc ataacgcaaa ggcgtggaga aaatctccgg
     6781 tgagacgcgc tccatgccat gagcaaacgc aaaattttct gccccttcgc caatcatcat
     6841 cacatgcggg ctttgctcca tcaccagccg ggcggcaaga accggattac gcagatgact
     6901 aacgcccgcc accgcaccgg ctttcagggt gttaccatcc atcacacagg cgtccagttc
     6961 atgggtttca tcacgcgtaa agacagcgcc aattccggcg ttaaacagtg gacactcttc
     7021 cagcagacgc accgcttccg tcaccacatc cagcgcactt tcgcccgctt ccagcatttt
     7081 ctgcccggtt tcaacaatgg cagacagcgc ctcgatgtag cgtaattcct gttgcagact
     7141 catctgcgcg cggctaattg cacctgcgcc gccatgaatt gcaatgactg ctttgcccat
     7201 agtattcgtc cattaagggt ggctcgccac ttttgctata aatatcagaa tttttatgaa
     7261 ttactcattc aatttttgaa tatagaaaga tggatatggc atgtaaaggc aggagccagc
     7321 gctacgcccg aggtggcata cagtctgtgg cgtttttctg acataatagg caaattcgat
     7381 tttgcctccg caggagtgtt ttcatggaat ttaccaccgg attgatgtcg ctcgacaccg
     7441 cgcttaatga gatgctttct cgcgttaccc cactgaccgc ccaggaaacg ctgccactgg
     7501 ttcagtgttt tggtcgtatt ctggcgagcg atgtcgtttc gccactggat gtcccggggt
     7561 ttgataactc cgctatggac ggctacgcgg tgcgtttagc cgatattgcc tccgggcaac
     7621 cgctgcccgt tgccggtaaa tcctttgccg gtcagccgta ccatggtgaa tggcctgcgg
     7681 gtacctgcat tcgtattatg accggtgcgc cggtgccaga aggctgcgaa ggggtggtga
     7741 tgcaggagca gactgaacaa acggacaatg gcgtgcgttt tactgctgaa gtgcgtagcg
     7801 ggcaaaatat tcgccgtcgc ggtgaagata tctctgcagg tgcggttgtt ttcccggcgg
     7861 ggactcgcct gactaccgct gaactgccag tgattgcttc gctagggatt gccgaagttc
     7921 cggtgattcg taaagtgcgt gtagcgcttt tttctaccgg tgatgaactc cagttgcccg
     7981 gtcagccgct gggcgacggc caaatctacg ataccaaccg tctcgccgta cacctgatgt
     8041 tagaacagtt gggatgcgag gtaatcaact tagggattat ccgcgacgat ccccatgccc
     8101 tgcgcgccgc atttattgaa gccgacagcc aggcggatgt ggtgatcagt tccggcggtg
     8161 tttcagtggg tgaagcggat tacaccaaaa ccattcttga agagctgggg gagatcgcct
     8221 tctggaagct ggcgattaaa ccaggtaaac cgttcgcgtt cggtaaactc agcaatagct
     8281 ggttctgcgg cctgccgggc aacccggttt cagcaacgct gaccttctat caactggtac
     8341 agcctttgct ggcaaaacta agcggcaata ccgccagcgg cctgcccgcg cgccagcgtg
     8401 tgcgcacggc atcgcctctg aagaaatcgc caggacgcct tgatttccag cgcggcgtgc
     8461 tgcaacgcaa cgccgatggc gaactggaag tgacgaccac cggacatcag ggttcacata
     8521 tatttagctc ctttagcctc ggcaactgct ttatcgtgct ggaacgcgat cgcggcaatg
     8581 tggaagtggg cgaatgggtg gaagtagaac cgtttaacgc gttgttcgga ggcctgtaat
     8641 ggcggaactc agcgatcagg agatgctgcg ctacaaccgg caaatcattc tgcgcggctt
     8701 tgattttgac ggccaggagg cgctgaaaga ttctcgcgtg ctggtagtgg gcctgggcgg
     8761 cctcggctgt gcagcctcgc agtatctggc aagcgccggt gtcggcaacc tgacgctgct
     8821 cgacttcgac acggtttcgc tctccaatct gcaacgccag acgctgcaca gtgatgccac
     8881 ggtcgggcaa ccgaaggtgg aatccgcccg tgacgccctg acgcggatca acccatatat
     8941 cgcgattacg ccagtcaatg cgctgcttga tgacgctgaa cttgctgcga tgattgcaaa
     9001 acacgatctg gtgctcgact gtacggataa cgttgcagta cgtaatcaac tgaacgcagg
     9061 ctgttttgcc gcgaaggtgc cgctggtttc cggcgcggca attcgtatgg aagggcaaat
     9121 cacggtcttc acttatcagg acggcgaacc gtgctatcgc tgccttagcc gtttgtttgg
     9181 cgagaacgcg ttaacctgcg tagaagcagg cgtgatggca ccgttaatcg gcgtaattgg
     9241 ttcgttgcag gcaatggaag caatcaaact gctggcaggt tatggcaaac cagccagcgg
     9301 gaaaatcgtg atgtacgatg cgatcacctg tcagtttcgc gaaatgaaac tgatgcgcaa
     9361 tccggggtgt gaggtgtgcg ggcagtaact gccacctcca ggccagataa gacgcggcac
     9421 tccgccacgt catcagcaat ttatgaggat gcagaacgtc cggttaaatc gacgttctgc
     9481 caaacgcacc cagccagtcc tgttcaaact tcgccacagc ggcatcaacc gccggatagc
     9541 taatcatctg ttgtgccaca tccagtggca gagtaattga ttcacatcct gccagtaagc
     9601 agtccagcgc ctgacgcggg gttttgaaac tcgctgccag cactttcgcc tgcggcgcat
     9661 gcattttcaa taactggtgt aagtcggtca cagtctgaat accgctaccg ccctgagcat
     9721 caatacgatt aacgtaaggc gcaacatatt ctgcacctgc cagcgccgac atcaaccctt
     9781 gtgccgcgcc atataccgcg gttcccagcg tcggaatccc ttccgctttt aacatcttaa
     9841 tcgctgccag cccctcggcg gtcaccggaa ctttcaccac gatatccgca ataatagaac
     9901 gcagcttaag cgcgtcattc accatccctt cggcagtggt agccattacc tgggcaaaca
     9961 gacgcccctg accgcccatc gcttcatgaa gttgcggaag cacaacctcc agcggttttt
    10021 tacccgcggc gataatgctt gggttagtgg tcacacccgc tagcggaaaa atacgtgaca
    10081 gcgccttcac cgcaacaacg tctgaagtat ccagatacag ttccataacc atcctcaaaa
    10141 tgtacttttt gtctgaatgt agactatcac gggaaaactc tctgaacagt tgacacacat
    10201 caatctaact ttcattcgaa agtattttaa tgtttgaacg aaacagagag gcaactatga
    10261 ttttcaatat tcagcgctac tcgacccatg atggccccgg tatccgcacg gtcgtatttc
    10321 ttaaaggctg ttcgctgggc tgccgctggt gtcagaaccc ggaaagccga gcccgcacgc
    10381 aggatctgtt gtatgacgca cgactgtgtc tggaaggctg cgagctgtgc gctaaggccg
    10441 cgccggacgt gattgagcgc tcgctgaatg gtttgcttat tcatcgggaa aagttaaccc
    10501 cggagcatct gacagcgtta accgactgct gtccgacaca ggcgttaacc gtgtgtggtg
    10561 aagtgaaaag cgttgaggag atcatggcga ccgttttgcg cgataaaccg ttttacgatc
    10621 gcagcggcgg cggcttaaca ctttctggcg gtgaaccctt tatgcagccg gaaatggcga
    10681 cggagctact gcaagccagc cacgaggccg gcattcatac tgcggtagaa acctgtctgc
    10741 atgtgccgtg gaaatatatc tccccttctc tgccctatat cgatctgttt cttgccgatt
    10801 taaaacacgt cgccgacgcg ccgtttaaac agtggaccga cggtaacgcc gccagggtgc
    10861 tggataatct gaaaaaactc gctgcggcgg ggaaaaaaat catcatccgc gtaccgctga
    10921 ttcagggctt taatgccgac gaaaccgctg taaaagccat taccgatttt gccgccgacg
    10981 agctgcacgt tggcgaaatc catttcctgc cctaccacac gctgggcatc aacaaatatc
    11041 acttacttaa tctgccctat gacgccccgg aaaaaccgct tgatgcgcca gaactgctcg
    11101 actttgccca gcagtatgcc tgccagaaag ggttaaccgc gaccttacga ggataacaac
    11161 catgaccaca ctgaaactgg acacgctcag cgaccgcatt aaagcgcaca aaaatgcgct
    11221 ggtgcatatt gtgaaaccac cagtctgtac cgagcgcgcg cagcactata ccgagatgta
    11281 tcaacaacat ctcgataagc cgatcccggt acgtcgcgcg ctggcactgg cgcatcacct
    11341 ggcgaatcgc accatctgga tcaagcacga tgaattgatc attggcaacc aggcaagcga
    11401 agttcgcgcc gcgccgatct tcccggaata taccgtctcc tggatcgaaa aagagattga
    11461 tgatctggca gatcgtcccg gtgctggttt tgcggtgagc gaagagaaca aacgcgttct
    11521 gcatgaagtg tgcccgtggt ggcgcggtca gaccgtacag gatcgctgct acggcatgtt
    11581 taccgatgag caaaaaggtc tgctggcgac cggcatcatt aaagcggagg gcaatatgac
    11641 ctccggcgat gcgcacctgg cggtcaattt cccgctactg ctggaaaaag gtcttgatgg
    11701 tctgcgtgag aaagtggcgg aacgtcgctc gcgcatcaat ctgacggtac tggaagatct
    11761 gcacggcgag caattcctga aagcgattga tatcgtgctg gtggcagtca gtgaacacat
    11821 tgaacgtttc gctgccctgg cgcgtgaaat ggccgcgacc gaaacccgcg aaagccgtcg
    11881 cgatgaactg ctgacgatag cagaaaactg cgatcttatc gcccaccagc cgccgcagac
    11941 tttctggcag gcgctgcaac tgtgttactt catccagttg attttgcaga tcgaatctaa
    12001 cggtcactca gtatcgtttg gtcgtatgga ccagtatctc tacccgtact atcgccgcga
    12061 cgttgaactg aaccagacac tggatcgtga acacgccatc gagatgctgc atagctgctg
    12121 gttaaaattg ctggaagtga acaagatccg ctccggctca cactcaaaag cctctgcggg
    12181 aagtccgctg tatcagaacg tcaccattgg cgggcaaaat ctggttgatg gtcaaccaat
    12241 ggacgcggtg aatccactct cttacgcgct cctcgaatcc tgcggtcgcc tgcgttccac
    12301 tcagcctaac ctcagcgtgc gttaccatgc aggaatgagc aacgatttcc tcgacgcctg
    12361 cgtacaggtg atccgttgcg gcttcgggat gccggcgttc aacaacgacg aaatcgtgat
    12421 cccggaattt attaaactcg gtattgaacc gcaggacgct tacgactatg cagcgattgg
    12481 ttgtatcgaa accgccgtcg gtggcaaatg gggctatcgc tgtaccggca tgagctttat
    12541 caacttcgcc cgtgtgatgc tggcggcgct ggaaggcggt cgtgatgcca ccagcggcaa
    12601 agtgttcctg ccacaagaaa aagcgttgtc ggcaggtagc ttcaacaact tcgatgaagt
    12661 gatggacgcg tgggatacgc aaatccgtta ctacacgcgt aaatcaatcg aaatcgaata
    12721 tgtcgtcgac accatgctgg aagagaacgt acacgatatt ctctgctcgg cgctggtgga
    12781 tgactgcatt gagcgagcga aaagtatcaa acaaggcggc gcgaagtatg actgggtgtc
    12841 tggcttgcag gtcggtatcg ccaacctcgg caacagcctg gcggcagtga agaaactggt
    12901 atttgaacag ggcgcgattg gtcagcaaca gctggctgcc gcactggcgg atgacttcga
    12961 cggcctgact cacgagcagc tgcgtcagcg gctgattaac ggtgcgccta agtacggcaa
    13021 cgacgatgat actgtcgata cgctgctggc tcgcgcttat cagacctata tcgacgaact
    13081 gaaacagtac cataatccgc gctacggtcg tggtccggtt ggcggcaact attacgcggg
    13141 tacgtcatct atctccgcta acgtaccttt tggcgcgcag acgatggcaa cgccggacgg
    13201 gcgtaaagcc cacaccccgc tggcagaagg cgcaagcccg gcctccggta ctgaccatct
    13261 tggccctacc gcggtcattg gctcggtggg taaactgcct acggcagcga ttctcggcgg
    13321 cgtgttgctc aaccagaaac tgaatccggc aacgctggag aacgaatctg acaagcagaa
    13381 gctgatgatc ctgctgcgca ccttcttcga ggtgcataaa ggctggcata ttcagtacaa
    13441 catcgtttcc cgcgaaacgc tgctggaggc gaaaaaacat cccgatcagt atcgcgatct
    13501 ggtagtgcgc gtcgcgggct attccgcctt cttcaccgcg ctctctccag acgctcagga
    13561 cgatatcatc gcccgtactg aacatatgct gtaatctcct cagcccggcg gcggcgtcgc
    13621 cgggctaaat cattcacatc atcaatttca gcctcgcttt cattcgaaat ataatttgtg
    13681 ctctgcgtca cattgttatt acactgttac ggtttcattt tcgagccagg agcacaccta
    13741 tgagcgtaaa agttatcgtc acagacatgg acggtacttt tcttaacgac gccaaaacgt
    13801 acaaccaacc acgttttatg gcgcaatatc aggaactgaa aaagcgcggc attgagttcg
    13861 ttgtcgccag cggtaatcag tattaccagc ttatttcgtt ctttcctgag ctaaaggatg
    13921 agatctcttt tgtcgcagaa aacggcgcac tggtttacga acatggcaag cagttgttcc
    13981 acggcgaact gacccgacat gaatcgcgga ttgttattgg cgagttgcta aaagataagc
    14041 aactcaattt tgtcgcctgc ggtctgcaaa gtgcatatgt cagcgaaaat gcccccgaag
    14101 cattcgtcgc actgatggca aaacactacc atcgcctgaa acctgtaaaa gattatcagg
    14161 agattgacga cgtactgttc aagttttcgc tcaacctgcc ggatgaacaa atcccgttag
    14221 tgatcgacaa actgcacata gcgctggatg gcattatgaa acccgtcacc agtggttttg
    14281 gctttatcga cctgattatt cccggtctac ataaagcaaa cggtatttcg cggttactga
    14341 aacgctggga tctgtcaccg caaaatgtgg tagcgattgg cgacagcggt aacgatgcgg
    14401 agatgctgaa aatggcgcgt tattcctttg cgatgggcaa tgctgcggaa aacattaaac
    14461 aaatcgcccg ttatgctacc gatgataata atcatgaagg cgcgctgaac gtgattcagg
    14521 ctgtgctgga taacacatcc ccttttaaca gctgacctct ctaccggagc catgcgctcc
    14581 ggttttccct tctttttaca tcctgtacat cacatcaaat cattaaactt gcattaactt
    14641 atttataact tatattgtca catgcaaatc agtatagttt cgaatgaaag ttaatgcgag
    14701 gtggttatgg cttctacttt taccagcgac acattgcctg ccgatcacaa agcagctatc
    14761 cgtcagatga agcacgcgct gcgggcgcag cttggcgacg tccagcagat ctttaatcag
    14821 ctaagcgatg acattgccac gcgagtggct gaaatcaacg cactcaaagc acagggcgat
    14881 gcagtctggc cggtgctgtc ttatgccgat atcaaagcag gtcatgttac tgcagagcaa
    14941 cgcgaacaga ttaaacgtcg cggttgtgcg gtgataaaag gccatttccc ccgcgaacaa
    15001 gcgctaggct gggatcagtc gatgctggac tatctggacc gcaaccgctt tgatgaggtc
    15061 tacaaaggcc ccggcgataa tttcttcggg acgctcagcg cttcacgtcc cgagatttac
    15121 cccatctact ggtcgcaggc gcaaatgcag gcccgccaga gtgaagaaat ggcgaatgcg
    15181 cagtcgtttc tcaatcgtct gtggacattt gaaagtgatg gaaagcaatg gtttaacccg
    15241 gatgtgagcg tcatctaccc tgaccgtatc cgccgccgtc cgcccggaac ggcctccaaa
    15301 ggtctcggag cgcataccga ctccggggca ctggaacgct ggctgcttcc agcgtatcag
    15361 cgcgttttcg ccaacgtctt taatggcaat ctggcgcaat atgatccctg gcatgcggca
    15421 catcgtacag aagttgaaga gtacacggtg gacaacacca ccaaatgttc cgtgtttcgg
    15481 acattccagg gctggacagc gctctctgat
//
LOCUS       DAMZFT010000117        15284 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.117, whole genome shotgun sequence.
ACCESSION   DAMZFT010000117 DAMZFT010000000
VERSION     DAMZFT010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15284)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15284)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15284
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.117"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            220..1374
                     /locus_tag="PN977_003780"
     CDS             220..1374
                     /locus_tag="PN977_003780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AgaS family sugar isomerase"
                     /protein_id="HDI6043779.1"
                     /translation="MPENYTPAAAATGTWTEEEIRHQPRAWIRSLTNIDALRSALNNF
                     LEPLLRKENLRVILTGAGTSAFIGDIIAPWLASHTGKNFSAVPTTDLVTNPMDYLNPA
                     HPLLLISFGRSGNSPESVAAVELANQFVPECYHLPITCNEAGALYQNAINSDNAFALL
                     MPAETHDRGFAMTSSITTMMASCLAVFAPETINSQSFRDVADRCQAILTSLGDFSEGV
                     FGYAPWKRIVYLGSGGLQGAARESALKVLELTAGKLAAFYDSPTGFRHGPKSLVDNET
                     LVVVFVSSHPYTRQYDLDLLAELRRDNQALRVIAIAAESNDVITAGPHIILPPSRHFI
                     DVEQAFCFLMYAQTFALMQSLHMGNTPDTPSASGTVNRVVQGVIIHPWQA"
     gene            1387..2247
                     /gene="kbaY"
                     /locus_tag="PN977_003781"
     CDS             1387..2247
                     /gene="kbaY"
                     /locus_tag="PN977_003781"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit KbaY"
                     /protein_id="HDI6043780.1"
                     /translation="MSIISTKYLLQDAQANGYAVPAFNIHNAETIQAILEVCSEMRSP
                     VILAGTPGTFKHIALEEIYALCSAYSTTYNMPLALHLDHHESLDDIRRKVHAGVRSAM
                     IDGSHFPFDENVKLVKSVVDFCHSQDCSVEAELGRLGGVEDDMSVDAESAFLTDPQEA
                     KRFVELTGVDSLAVAIGTAHGLYSKTPKIDFQRLAEIREVVDVPLVLHGASDVPDEFV
                     RRTIELGVTKVNVATELKIAFAGAVKAWFAENPQGNDPRYYMRVGMDAMKEVVRNKIN
                     VCGSANRISA"
     gene            2414..2890
                     /gene="agaB"
                     /locus_tag="PN977_003782"
     CDS             2414..2890
                     /gene="agaB"
                     /locus_tag="PN977_003782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIB"
                     /protein_id="HDI6043781.1"
                     /translation="MSSPNILLTRIDNRLVHGQVGVTWTSTIGANLLVVVDDVVANDD
                     IQQKLMGITAETYGFGIRFFTIEKTINVIGKAAPHQKIFLICRTPQTVRKLVEGGIDL
                     KDVNVGNMHFSEGKKQISSKVYVDDQDLADLRFIKQRGVNVFIQDVPGDQKEQIPD"
     gene            2929..3732
                     /gene="agaC"
                     /locus_tag="PN977_003783"
     CDS             2929..3732
                     /gene="agaC"
                     /locus_tag="PN977_003783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIC"
                     /protein_id="HDI6043782.1"
                     /translation="MHEITLLQGLSLAALVFFLGIDFWLEALFLFRPIIVCTLTGAIL
                     GDIQTGLITGGLTELAFAGLTPAGGVQPPNPIMAGLMTTVIAWSTGVDAKTAIGLGLP
                     FSLLMQYVILFFYSAFSLFMTKADKCAKEADTAAFSRLNWTTMLIVASAYAVIAFLCT
                     YLAQGAMQALVKAMPAWLTHGFEVAGGILPAVGFGLLLRVMFKAQYIPYLIAGFLFVC
                     YIQVSNLLPVAVLGAGFAVYEFFNAKSRQQAQPQPVASKNEEEDYSNGI"
     gene            3722..4513
                     /gene="agaD"
                     /locus_tag="PN977_003784"
     CDS             3722..4513
                     /gene="agaD"
                     /locus_tag="PN977_003784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IID"
                     /protein_id="HDI6043783.1"
                     /translation="MGSEISKKDITRLGFRSSLLQASFNYERMQAGGFTWAMLPILKK
                     IYKDDKPGLSAAMKDNLEFINTHPNLVGFLMGLLISMEEKGENRDTIKGLKVALFGPI
                     AGIGDAIFWFTLLPIMAGICSSFASQGNLLGPILFFAVYLLIFFLRVGWTHVGYSVGV
                     KAIDKVRKNSQMIARSATILGITVIGGLIASYVHINVVTSFAIDSTHSVALQQDFFDK
                     VFPNILPMAYTLLMYYFLRVKKAHPVLLIGVTFVLSIVCSAFGIL"
     gene            4514..5281
                     /locus_tag="PN977_003785"
     CDS             4514..5281
                     /locus_tag="PN977_003785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019843039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactosamine-6-phosphate isomerase"
                     /protein_id="HDI6043784.1"
                     /translation="MERGTASGGASLLKEFHPVQTLQQVENYTALSERASEYLLAVIR
                     SKPDAVICLATGATPLLTYHYLVEKIHQQQVDVSQLTFVKLDEWVDLPLTMPGTCETF
                     LQQHIVQPLGLREDQLISFRSEEINETECERVTNLIARKGGLDLCVLGLGKNGHLGLN
                     EPGESLQPACHISQLDARTQQHEMLKTAGRPVTRGITLGLKDILNTREVLLLVTGEGK
                     QDATERFLTAKVSTAIPASFLWLHNHFTCVIDEMCRR"
     gene            complement(5331..6194)
                     /gene="rsmI"
                     /locus_tag="PN977_003786"
     CDS             complement(5331..6194)
                     /gene="rsmI"
                     /locus_tag="PN977_003786"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000809248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HDI6043785.1"
                     /translation="MKQHQSADNSQGQLYIVPTPIGNLADITQRALEVLQAVDLIAAE
                     DTRHTGLLLQHFGINARLFALHDHNEQQKAETLLAKLQEGQNIALVSDAGTPLINDPG
                     YHLVRTCREAGIRVVPLPGPCAAITALSAAGLPSDRFCYEGFLPAKSKGRRDALKAIE
                     AEPRTLIFYESTHRLLDSLEDIVAVLGESRYVVLARELTKTWETIHGAPVGELLAWVK
                     EDENRRKGEMVLIVEGHKAQEEDLPADALRTLALLQAELPLKKAAALAAEIHGVKKNA
                     LYKYALEQQGE"
     gene            6259..8295
                     /gene="lpoA"
                     /locus_tag="PN977_003787"
     CDS             6259..8295
                     /gene="lpoA"
                     /locus_tag="PN977_003787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoA"
                     /protein_id="HDI6043786.1"
                     /translation="MVPSTFSRLKAARCLPVVLAALIFAGCGTHTPDQSTAYMQGTAQ
                     ADSAFYLQQMQQSSDDTRINWQLLAIHALVKEGKTGQAVELFNQLPQELNDSQRREKT
                     LLAAEIKLAQKDFAGAQNLLAKITPADLEQNQQARYWQAKIDASQGRPSIDLLRALIA
                     QEPLLGAKEKQQNIDATWQALSSMTQEQANTLVINADENILQGWLDLQRIWFDNRNDP
                     DMMKAGIADWQKRYPNNPGAKMLPTQLVNVKAFKPASTNKIALLLPLNGQAAVFGRTI
                     QQGFEAAKNIGTQPVAAQVAAAPAADVAEQPQPQTVDGVASPAQASVSDLTGEQPAAQ
                     PVPVSAPATSTAAVSAPANPSAELKIYDTSSQPLSQILSQVQQDGASIVVGPLLKNNV
                     EELLKSNTPLNVLALNQPENIENRVNICYFALSPEDEARDAARHIRDQGKQAPLVLIP
                     RSSLGDRVANAFAQEWQKLGGGTVLQQKFGSTSELRAGVNGGSGIALTGSPITPRETT
                     DSGMTTNNPTLQTTPTDDQFTNNGGRVDAVYIVATPGEIAFIKPMIAMRNGSQSGATL
                     YASSRSAQGTAGPDFRLEMEGLQYSEIPMLAGGNLPLMQQALSAVNNDYSLARMYAMG
                     VDARSLANHFSQMRQVQGFEINGNTGSLTANPDCVINRKLSWLQYQQGQVVPAS"
     gene            8253..8648
                     /locus_tag="PN977_003788"
     CDS             8253..8648
                     /locus_tag="PN977_003788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HDI6043787.1"
                     /translation="MATVPTRSGSPRQLTTKQTGDAWEAQARRWLEGKGLRFIAANVN
                     ERGGEIDLIMREGRTTVFVEVRYRRSALYGGAAASVTRSKQHKLLQTARLWLARHNGS
                     FDTVDCRFDVVAFTGNEVEWIKDAFNDHS"
     gene            8668..9258
                     /gene="diaA"
                     /locus_tag="PN977_003789"
     CDS             8668..9258
                     /gene="diaA"
                     /locus_tag="PN977_003789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001483830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA initiator-associating protein DiaA"
                     /protein_id="HDI6043788.1"
                     /translation="MQERIKACFTESIQTQIAAAEALPDAISRAAMTLVQSLLNGNKI
                     LCCGNGTSAANAQHFAASMINRFETERPSLPAIALNTDNVVLTAIANDRLHDEVYAKQ
                     VRALGHAGDVLLAISTRGNSRDIVKAVEAAVTRDMTIVALTGYDGGELAGLLGPQDVE
                     IRIPSHRSARIQEMHMLTVNCLCDLIDNTLFPHQDV"
     gene            9268..9843
                     /gene="yraP"
                     /locus_tag="PN977_003790"
     CDS             9268..9843
                     /gene="yraP"
                     /locus_tag="PN977_003790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divisome-associated lipoprotein YraP"
                     /protein_id="HDI6043789.1"
                     /translation="MKALSPIAVLLSALLLQGCVAAAVVGTAAVGTKAATDPRSVGTQ
                     VDDGTLEVRVNSALSKDEQIKKETRINVTAYQGKVLLVGQSPNAELSARAKQIAMGVD
                     GANEVYNEIRQGQPIGLGEASNDTWITTKVRSQLLTSDLVKSSNVKVTTENGEVFLMG
                     LVTEREAKAAADIASRVSGVKRVTTAFTFIK"
     gene            complement(9956..10996)
                     /locus_tag="PN977_003791"
     CDS             complement(9956..10996)
                     /locus_tag="PN977_003791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001551701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HDI6043790.1"
                     /translation="MTGQSSSQAATPIQWWKPALFFLVVIAGLWYVKWQPYYGKAFTA
                     AETHSIGKSILAQADANPWQAALDYAMIYFLAVWKAAVLGVLLGSLIQVLIPRDWLLR
                     TLGQSRFRGTLLGTLFSLPGMMCTCCAAPVAAGMRRQQVSMGGALAFWMGNPLLNPAT
                     LVFMGFVLGWGFAAIRLVAGLVMVLLIATLVQKWVRETPQTQAPVEIDIPEAQGGFFS
                     RWGRALWTLFWSTIPVYILAVLVLGAARVWLFPHADGAVDNSLMWVVAMAVAGCLFVI
                     PTAAEIPIVQTMILAGMGTAPALALLMTLPAVSLPSLIMLRKAFPAKALWLTGAMVAV
                     SGVIVGGLALLA"
     gene            complement(11069..11704)
                     /locus_tag="PN977_003792"
     CDS             complement(11069..11704)
                     /locus_tag="PN977_003792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312060.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HDI6043791.1"
                     /translation="MSQVLITGATGLVGGHLLRMLINEPKVNAIAAPTRRPLGDMPGV
                     FNPHDPQLTDALAQVTDPIDIVFCCLGTTRREAGSKEAFIHADYTLVVDTALTGRRLG
                     AQHMLVVSAMGANAHSPFFYNRVKGEMEEALIAQNWPKLTIARPSMLLGDRSKQRMNE
                     TLFAPLFRLLPGNWKSIDARDVARVMLAEAMRPEHEGVTILSSSELRKRAE"
     gene            11832..12350
                     /gene="yhbO"
                     /locus_tag="PN977_003793"
     CDS             11832..12350
                     /gene="yhbO"
                     /locus_tag="PN977_003793"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein/nucleic acid deglycase"
                     /protein_id="HDI6043792.1"
                     /translation="MSKKIAVLISDEFEDSEFTSPADEFRKAGHEVITIEKQAGKTVK
                     GKKGEASVAIDKSIDEVTPAEFDALLLPGGHSPDYLRGDNRFVTFTRDFVNSGKPVFA
                     ICHGPQLLISADVIRGRKLTAVKPIIIDVKNAGAEFYDQEVVVDKDQLVTSRTPDDLP
                     AFNREALRLLGA"
     gene            complement(12330..12773)
                     /locus_tag="PN977_003794"
     CDS             complement(12330..12773)
                     /locus_tag="PN977_003794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhbP family protein"
                     /protein_id="HDI6043793.1"
                     /translation="METLTAISRWLAKQHVVTWCVQQEGELWCANAFYLFDAQKVAFY
                     ILTEEKTRHAQMSGPQAAVAGTVNGQPKTVALIRGVQFKGEIRRLEGEESERARQAYN
                     RRFPVARMLSAPVWEIRLDEIKFTDNTLGFGKKMIWLRNSGTEQA"
     gene            12824..13126
                     /gene="yhbQ"
                     /locus_tag="PN977_003795"
     CDS             12824..13126
                     /gene="yhbQ"
                     /locus_tag="PN977_003795"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage response exodeoxyribonuclease YhbQ"
                     /protein_id="HDI6043794.1"
                     /translation="MTPWFLYLIRTADNKLYTGITTDVERRYQQHQSGKGAKALRGKG
                     ELTLAFSAPVGDRSLALRAEYRVKQLTKRQKERLVAEGAGFAELLSSLQTPKIKSD"
     gene            complement(13113..13616)
                     /locus_tag="PN977_003796"
     CDS             complement(13113..13616)
                     /locus_tag="PN977_003796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HDI6043795.1"
                     /translation="MLIRVEIPIDAPGIDALLRRSFESDAEAKLVHDLREDGFLTLGL
                     VATDDEGQVIGYVAFSPVDVQGEDLQWVGMAPLAVDEKYRGQGLARQLVYEGLDSLNE
                     FGYAAVVTLGDPALYSRFGFELAAHHDLRCRWPGTESAFQVHRLADDALNGVTGLVEY
                     HEHFNRF"
     gene            complement(13610..14134)
                     /locus_tag="PN977_003797"
     CDS             complement(13610..14134)
                     /locus_tag="PN977_003797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 domain-containing protein"
                     /protein_id="HDI6043796.1"
                     /translation="MLDKLRSRIVHLGPSLLSVPVKLTPFALKRQVLEQVLSWQFRQA
                     LDDGELEFLEGRWLSIHVRDIDLQWFTSVVNGKLVVSQNAQADVSFSADASDLLMIAA
                     RKQDPDTLFFQRRLVIEGDTELGLYVKNLMDAIELEQMPKALRMMLLQLADFVEAGMK
                     TAPETKQTSVGEPC"
     gene            14343..>15284
                     /locus_tag="PN977_003798"
     CDS             14343..>15284
                     /locus_tag="PN977_003798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017457364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HDI6043797.1"
                     /translation="MELLCPAGNLPALKAAIENGADAVYIGLKDDTNARHFAGLNFTE
                     KKLQEAVSFVHQHRRKLHIAINTFAHPDGYARWQRAVDMAAQLGADALILADLAMLEY
                     AAERYPHIERHVSVQASATNEEAINFYHRHFDVARVVLPRVLSIHQVKQLARVTPVPL
                     EVFAFGSLCIMSEGRCYLSSYLTGESPNTVGACSPARFVRWQQTPQGLESRLNEVLID
                     RYQDGENAGYPTLCKGRYLVDGERYHALEEPTSLNTLELLPELMAANIASVKIEGRQR
                     SPAYVSQVAKVWRQAIDRCKADPQNFIPQSAWMETLGS"
BASE COUNT         3728 a         4119 c         3903 g         3534 t
ORIGIN      
        1 tcggtgaaca tagcgacgtt atcacgcaat cctttaatct tcccttgtct tttctttcaa
       61 tgatcacaaa tttcgtttta tttctttttt ctccattgaa ctttcagttt cttttctata
      121 gatttttatc aacgaaagac atcaccaagt gaaatgaaac gaaaggtaag cgaaagcgac
      181 aacgcccgac gtcaagttca tcagactaag gattgagtta tgccagaaaa ttacacccct
      241 gctgctgccg caaccggtac atggactgaa gaagagatcc gccatcagcc tcgcgcatgg
      301 atccgttcac tcaccaacat cgacgcgcta cgttccgcgc tcaataactt ccttgaaccg
      361 ttactgcgca aagagaatct gcgggtaatc ctgaccggag ccggaacctc ggcatttatc
      421 ggtgacatca tcgcgccgtg gctcgccagc cataccggta aaaacttcag cgccgtaccg
      481 accaccgatc tggtcactaa tccgatggac tacctgaatc cagctcatcc gctgctgttg
      541 atctccttcg gtcgatccgg caacagcccg gaaagcgtcg ccgccgtgga actggcaaat
      601 caatttgtac cagaatgcta tcacctgccg atcacctgca acgaagcggg cgctctttac
      661 caaaacgcga tcaacagcga caacgcgttt gccctgctga tgcccgcaga aacgcacgat
      721 cgcggcttcg cgatgaccag cagcattacc accatgatgg ccagctgcct cgcggttttc
      781 gcacctgaga cgatcaacag ccagagcttc cgcgatgtgg cggatcgttg ccaggcgatc
      841 ctgacctcac tgggcgattt cagcgaaggt gtgtttggtt acgcaccgtg gaaacggatc
      901 gtttatctcg gcagcggtgg cttacagggc gcagcacgcg agtcggcgct gaaagtgctg
      961 gaactgacgg cgggtaaact ggcggccttt tatgattccc cgaccggatt ccgtcatggc
     1021 ccgaaatcgc tggtcgataa cgaaacgctg gtggtggtgt ttgtctccag ccacccttac
     1081 acccgtcagt atgatcttga tctgctggca gaactccgcc gtgacaacca ggcattgcgc
     1141 gtaatcgcca tcgccgcgga aagcaacgac gttattaccg ccggtccaca tatcatcctg
     1201 ccgccgtccc gtcactttat cgacgttgag caggcatttt gcttcctgat gtacgcccag
     1261 acgtttgcac tgatgcagtc gctgcacatg ggcaatacgc cggatacccc atcagccagt
     1321 ggcaccgtta accgcgtggt gcaaggcgta atcattcatc cgtggcaggc ataagaggat
     1381 cgcattatga gcattatctc caccaaatat ctgttacagg acgcccaggc caatggctac
     1441 gcggtgcctg cctttaacat tcataacgcc gagacgatcc aggcgatcct cgaagtgtgc
     1501 agtgaaatgc gatcgccggt gatcctcgcc gggacgccgg ggacctttaa acatatcgcg
     1561 ctggaagaga tctacgccct gtgtagtgcc tattccacaa cctacaacat gccactggcg
     1621 ctgcatctcg accaccacga atcgctggat gatattcgcc gtaaagtcca cgcaggcgtg
     1681 cgcagtgcga tgatcgacgg cagccacttc ccgtttgacg agaacgtaaa gctggtgaaa
     1741 tcggttgtcg acttctgcca ctcacaggat tgcagcgtgg aagcagaact gggccgcctc
     1801 ggcggtgttg aagatgacat gagcgttgac gccgaaagtg cattcctgac cgatccacaa
     1861 gaagctaaac gctttgtcga actgactggc gtcgacagcc tggcggtagc gattggtacg
     1921 gcgcacggct tatacagcaa aacgccgaag attgatttcc agcggctggc ggaaattcgt
     1981 gaagtggtgg atgttcctct ggtgctgcat ggtgccagcg atgtcccgga tgaatttgtc
     2041 cgtcgcacta ttgaacttgg cgtcacaaaa gtgaacgttg ccacagaatt aaaaatagcc
     2101 ttcgctggcg cggttaaagc ctggtttgcg gaaaatccgc agggtaatga tcctcgttat
     2161 tatatgcgcg tcggaatgga tgcgatgaaa gaagttgtca gaaataaaat taatgtctgt
     2221 ggttcagcga atcgaatttc agcataatca ttagtttttt atgatttatc ccaatgtact
     2281 tcccgattaa tatcggggag tgccttaatg gaaaaggaga tgactaaacc ttaataaata
     2341 catcagtaca atatcgcaac aataatatat ttaaaaaaat tatattattc aactttatgg
     2401 tgaggattac acaatgagca gtccaaatat tctcttaacc cgtattgata accgtctggt
     2461 tcacggtcag gttggcgtga cctggacatc caccatcggt gcgaatctgt tggtagtcgt
     2521 ggatgatgtt gtcgctaacg atgatattca acagaaatta atgggtatta ccgcagaaac
     2581 ctacggcttt ggcattcgtt tctttactat cgaaaaaacc attaacgtca tcggcaaagc
     2641 tgcaccgcat cagaagatct tcctgatttg ccgtacgcca caaacggtac gtaaattggt
     2701 agaaggtggt attgacctga aagatgtcaa cgtcggcaat atgcatttct cggaagggaa
     2761 aaagcaaatc agcagtaaag tttatgtcga tgaccaggat ctcgcggact tacgttttat
     2821 taaacaacgt ggcgtgaatg ttttcattca ggacgtccct ggcgatcaaa aagaacaaat
     2881 ccctgactaa atctaaaatc gccttaatat tggtttgagg taataaaaat gcatgaaata
     2941 accctactcc agggattatc cctggcggcg ttagttttct ttctggggat tgatttttgg
     3001 ctggaagcct tatttttatt ccgcccgata atcgtttgta cccttacagg tgctattctc
     3061 ggtgatattc agactggctt aattaccggt ggcctgacag agttggcttt cgccggatta
     3121 acccctgcag gtggtgttca gccgcccaac cctattatgg cgggtctgat gaccactgtc
     3181 attgcatggt ctacgggcgt tgatgccaaa acggcaattg gtcttggcct gccgtttagt
     3241 ttgttaatgc agtacgtcat tctgttcttc tattccgctt tctcattatt tatgaccaaa
     3301 gccgataaat gcgcaaaaga ggcggatacg gcagcatttt cccgacttaa ctggacaacg
     3361 atgctcatcg tcgcttcagc gtatgcggtg attgctttcc tctgtactta cctggcacaa
     3421 ggggcgatgc aggcgctggt gaaagcgatg cccgcctggc tgacccacgg ctttgaagtg
     3481 gctggcggta ttctgcctgc cgttggtttt ggcttgctgc tgcgcgtgat gttcaaagcg
     3541 caatatatcc cttacctgat cgccggtttc ctgttcgttt gctacatcca ggtcagcaac
     3601 ctgttgccgg ttgccgtgct gggcgcaggc tttgcggtgt atgagttttt caatgcgaaa
     3661 tcccggcagc aagcgcaacc gcagcccgtt gccagtaaaa atgaagaaga ggactacagc
     3721 aatgggatct gaaatcagta aaaaagatat cacccgtctg ggctttcgtt cgtcactgct
     3781 gcaagcgagc tttaactacg aaaggatgca ggcgggcggt tttacctggg cgatgttgcc
     3841 gatcctgaaa aagatttata aggacgacaa accgggccta agcgcggcga tgaaagataa
     3901 cctcgaattt attaataccc acccgaatct ggtcggattc ctgatggggt tattaatttc
     3961 gatggaagaa aaaggagaaa accgcgacac cattaaaggc ctcaaagtgg cactgtttgg
     4021 cccaatcgcc gggattggcg atgcgatttt ctggtttacc ttattgccga ttatggcggg
     4081 aatttgctca tcatttgcca gccagggaaa cctgttgggg ccgattttgt ttttcgccgt
     4141 ttacctgctt atcttcttcc tgcgcgtcgg ctggacccac gtcggttatt cagtcggcgt
     4201 gaaggcgatc gataaagtgc gaaagaactc gcagatgatt gctcgttcgg caaccatcct
     4261 cgggatcacg gtaatcggcg ggctgatcgc ttcgtatgtg catattaacg tggtgacatc
     4321 gtttgccatc gacagtaccc acagcgtcgc actgcagcag gatttcttcg ataaagtctt
     4381 cccgaacatt ttaccgatgg cctacaccct gctgatgtat tacttcctgc gggtgaaaaa
     4441 agcgcatccg gtgctgttaa tcggcgtgac ttttgtgctc tctattgttt gttccgcatt
     4501 cggcattttg taaatggaac gaggcactgc gtctggcggt gcctctttac ttaaggaatt
     4561 tcatcctgtg caaacccttc aacaagttga aaactatacg gcgttaagtg aacgtgccag
     4621 cgaatattta ttggccgtga tccgtagtaa accggatgcc gtgatttgcc tggcgaccgg
     4681 agccacgcca ttactgacgt atcattatct ggtagaaaaa atccaccagc agcaggttga
     4741 tgtcagccag ctcaccttcg tgaagctcga cgaatgggtg gatctgccat taacaatgcc
     4801 tggcacctgc gaaactttcc tacaacagca tatcgtgcag ccgctggggc tgcgtgaaga
     4861 ccagctcatc agctttcgct ctgaagagat aaatgagaca gagtgcgaac gggtaacgaa
     4921 cctgattgcg cgcaaaggcg gtctggattt atgcgttctc ggattgggga aaaacggtca
     4981 tcttgggctg aacgaaccgg gagaaagcct gcaaccggcc tgccatatca gtcaacttga
     5041 tgccagaaca cagcaacatg agatgttaaa aaccgcgggc cgtcccgtga ctcgtgggat
     5101 caccttaggc ctgaaggata ttctcaatac ccgcgaagtt ttgctactgg tgactggcga
     5161 aggaaagcag gatgcgacag aacgttttct cacggctaaa gtctctaccg ctatcccggc
     5221 ttcgttttta tggctgcata accatttcac atgcgtgatt gatgaaatgt gtcgtcgtta
     5281 actcgcctgg taaaggcgtt gtagcgctat tcctgctgca acgcctgtaa tcactctccc
     5341 tgctgctcca gtgcatactt atacagcgca tttttcttca cgccgtgaat ttctgctgcc
     5401 agcgccgccg cttttttcag tggcagttct gcctgcaaca gcgccagcgt gcgcagggca
     5461 tcggcgggta agtcttcttc ctgtgcttta tggccttcga caatcagtac catttcgcct
     5521 ttgcgacggt tttcatcttc tttgacccac gccagcagct cgccaacggg cgcgccgtga
     5581 atggtttccc aggttttggt cagctcacgc gccagaacga cgtagcggga ttcgcctaat
     5641 accgcaacga tatcttccag gctatctaac aggcggtggg tagattcata aaaaatcagc
     5701 gtgcgcggct ccgcttcaat ggcttttagc gcatcacggc ggccttttga tttggcaggt
     5761 aaaaagcctt cataacaaaa acgatcggat ggtaaacccg cggcgcttaa cgcagtgata
     5821 gcagcacacg gcccgggtag cggcaccacg cgaatccccg cttcacggca ggtacgcacc
     5881 agatggtagc caggatcgtt aattagcggc gttccggcat cggaaaccag cgcaatgttt
     5941 tgcccctctt gcagcttcgc cagcagcgtt tcggcttttt gttgttcgtt atggtcgtgc
     6001 agcgcaaaca accgagcatt aatcccaaaa tgttgcagca ataaaccggt gtgacgcgta
     6061 tcctcggcgg caatcagatc aacggcctgt aatacctcca acgcacgctg ggtgatatcc
     6121 gccagattgc cgattggcgt cggtacaatg taaagctggc cctgagaatt atccgccgat
     6181 tggtgttgtt tcattgtgtc gtccgtattg ccgatttaat attgagcatt gcgtaaaaaa
     6241 aatatcactg gatacattat ggtaccctca acattttctc gtttgaaagc cgcgcgttgt
     6301 ctgcctgttg ttctggcagc cctgattttc gccggttgtg gcacccatac tcccgatcag
     6361 tccactgctt atatgcaggg cacggcgcag gctgattctg ccttttacct gcaacagatg
     6421 cagcaaagtt ctgatgatac caggatcaac tggcaattac tcgccattca tgcactggtg
     6481 aaagaaggta aaaccggaca ggcggtggag ttgtttaacc aactaccgca agaactgaac
     6541 gattctcagc gtcgcgagaa aacactgctg gcggcagaga ttaaactggc gcagaaagat
     6601 tttgctggcg cgcaaaactt gctggcgaaa atcacacctg ccgatttaga acaaaaccag
     6661 caagcgcgtt actggcaggc aaaaatcgat gccagccagg ggcgtccttc cattgattta
     6721 ctgcgcgcgt taattgctca ggaaccgctg ctcggcgcga aagaaaagca gcagaatatt
     6781 gacgccacct ggcaggcgct ctcctccatg actcaggaac aggcgaatac gctcgtgatc
     6841 aatgccgacg aaaatattct gcaaggctgg ttggatctgc agcgcatctg gtttgataac
     6901 cgtaacgatc ccgacatgat gaaagccggg atcgccgact ggcagaaacg ttatccgaac
     6961 aacccgggcg cgaaaatgct gccaacgcag ttggttaacg taaaagcgtt taaacccgca
     7021 tcgaccaaca aaatcgccct gctgttgcca ctgaatggcc aggcagcggt atttggtcgc
     7081 actattcagc aaggctttga agcggcgaaa aatatcggca ctcagccagt ggcggcccag
     7141 gtagctgccg cacctgccgc agacgtagca gaacaacctc agccgcaaac cgtggatggc
     7201 gttgccagcc cggcacaagc ctcggttagc gatctgaccg gtgagcagcc tgcggcccag
     7261 ccggtgcctg taagcgcccc ggcgacaagc accgcagcgg taagcgcacc cgcaaatcca
     7321 tccgcagagc taaaaatcta cgatacctca tcacaaccac ttagccagat cttaagccag
     7381 gttcagcagg atggtgcgag tattgtggtc ggtccgttgc tgaaaaataa cgttgaagag
     7441 ttgctgaaga gcaacacccc gctgaacgtg ctggcactga accagccgga gaatatcgaa
     7501 aaccgcgtca atatttgtta cttcgcgctt tcaccggaag acgaagcgcg cgatgcagcg
     7561 cgtcatattc gtgaccaggg taaacaagcg ccgctggtgc tgatcccacg cagttcattg
     7621 ggcgatcgcg tagccaatgc gtttgcgcaa gagtggcaga aactgggtgg cggcaccgtt
     7681 ctgcaacaaa aatttggttc caccagcgaa ttacgcgcgg gtgttaacgg cggttctggt
     7741 attgctttaa cgggtagccc gattactccc agagaaacaa ccgactccgg catgacgacc
     7801 aacaatccaa cgctgcaaac cacgccaacc gatgaccagt tcaccaataa tggcggtcgt
     7861 gtcgatgcgg tgtacattgt ggcaacgccg ggtgaaatcg cttttatcaa accaatgatc
     7921 gccatgcgta acggtagcca gagcggtgca acgctgtacg ccagctcccg cagtgcgcaa
     7981 gggaccgctg gcccggattt ccgcctggag atggaaggct tgcagtacag cgaaatcccg
     8041 atgctggcag gcggtaatct gccgttaatg cagcaggcgc tcagcgcggt gaacaacgat
     8101 tattcgctgg ctcgcatgta tgcgatgggc gtcgatgccc ggtcgttggc aaatcatttc
     8161 tcacaaatgc gccaggttca gggttttgaa atcaacggta ataccggaag tctgacggct
     8221 aacccggatt gcgtgattaa caggaagtta tcatggctac agtaccaaca aggtcaggta
     8281 gtccccgcca gttaaccacc aaacagaccg gcgatgcgtg ggaagcacaa gcgcgtcgct
     8341 ggctggaagg caaaggactg cggtttatcg ccgctaacgt gaacgagcgt ggcggcgaga
     8401 tcgatctgat aatgcgtgaa ggcaggacca ccgtttttgt cgaggtgcgt taccgccgct
     8461 ctgcgcttta tggcggcgcg gcagccagtg tgacccgcag caaacaacac aaattattac
     8521 agactgcccg cttgtggctc gcgcgtcata atgggagttt tgatactgtg gattgccggt
     8581 tcgatgtggt agccttcacc gggaatgagg ttgagtggat taaggatgcc tttaatgacc
     8641 actcataatt aaggtttaag gattagcgtg caagaaagaa ttaaagcttg cttcactgaa
     8701 agcattcaaa ctcaaattgc ggcggcagag gcgcttccgg atgccatctc gcgtgcagct
     8761 atgacgctgg ttcagtctct gctcaatggc aacaaaatcc tctgttgtgg taatggaact
     8821 tccgctgcca atgcacagca ttttgctgcc agcatgatca accgtttcga aacggagcgg
     8881 cccagcttac ctgccattgc actaaatact gataatgttg tcttaacggc gattgccaac
     8941 gatcgcttac atgatgaagt gtatgcaaaa caggtgcggg cgctgggtca tgcgggagat
     9001 gtattgttag ccatttccac ccgtggcaac agccgcgata ttgtaaaagc ggtagaagct
     9061 gccgttacgc gtgatatgac cattgtggca ttaaccggct atgacggcgg tgaacttgca
     9121 ggtttgttag ggccacagga tgtggagatc cgcattcctt cgcatcgtag tgctcgcatt
     9181 caggaaatgc atatgctgac ggtaaattgc ctgtgcgatc tgatcgataa cacgcttttc
     9241 cctcaccagg atgtttaagg agaatacatg aaggcattat cgccaatcgc agtccttctt
     9301 tccgcgctgc tgttgcaagg ttgtgttgcc gctgccgtag tgggtaccgc tgctgtaggt
     9361 accaaagccg caactgaccc acgcagtgtc ggcacccagg tggacgatgg taccctggaa
     9421 gtgcgcgtga acagcgcatt gtcgaaagac gaacagatta agaaagaaac ccgtattaat
     9481 gtcacggcgt atcagggcaa agtgctgttg gttgggcagt caccaaacgc tgaactttcg
     9541 gctcgcgcta aacaaattgc gatgggcgta gacggtgcca acgaagtgta taacgagatt
     9601 cgtcagggcc agccgattgg tctgggcgaa gcgtctaacg atacgtggat caccaccaaa
     9661 gtgcgttcgc agctgttaac cagcgacctg gtgaaatcgt ccaacgtgaa agtgaccacc
     9721 gaaaacggtg aagtgttcct gatggggctg gtgactgaac gtgaagcgaa agcggcggca
     9781 gatattgcca gccgggtgag cggcgtaaag cgtgtaacta cggcgtttac gtttattaaa
     9841 tagcagccct tgtaatgcct gatgcgacgc ttgcagcgtc ttatcaggcc tacaacgatg
     9901 caaaccgtag gtcggataag gcgtttacgc cgcatccgac atgttttccc tcaaatcaag
     9961 ccaacagcgc caacccgccg acaatcacac cagacactgc caccatcgcc cccgtcagcc
    10021 ataaggcttt cgccgggaac gctttacgca acataatcag tgacggcaaa ctcaccgccg
    10081 ggagcgtcat caacaatgcc agcgccggag cggttcccat gcctgccagt atcatcgttt
    10141 gtacaatcgg aatttctgct gccgtgggaa tgacaaacaa gcatcctgcc accgccatcg
    10201 ccaccaccca catcaggctg ttatcgacag caccatcggc atgggggaat agccagacgc
    10261 gagcggcacc caacaccagt actgcaagga tgtaaaccgg gattgtactc cagaaaagcg
    10321 tccatagcgc cctgccccag cggctaaaaa atcctccctg tgcttccggt atgtcaattt
    10381 cgaccggtgc ctgcgtttgc ggtgtttcac gcacccattt ttgcaccagc gtcgcaatca
    10441 gcaacaccat caccagcccc gccaccagac gaatcgccgc aaaaccccag ccgaggacga
    10501 accccataaa caccagcgtc gccgggttta acaacggatt ccccatccag aatgccagcg
    10561 caccgcccat cgacacctgt tggcgacgca ttcccgccgc gaccggagcc gcacagcagg
    10621 tacacatcat gcccggcagc gaaaacagcg ttcccagcag cgtgccgcga aagcgcgatt
    10681 gcccaagcgt acgcaataac cagtcacgcg ggatcagcac ctgaatcaac gacccgagca
    10741 gtacgcccag caccgccgct ttccataccg cgaggaaata gatcatcgcg taatccaacg
    10801 ccgcctgcca cggattagca tccgcctgcg caaggataga tttaccgata ctgtgggttt
    10861 cggcagcagt aaacgctttg ccgtagtaag gttgccattt cacataccag agaccagcaa
    10921 tgacaacgag aaagaaaagc gcgggtttcc accactgaat gggcgttgcc gcctgagatg
    10981 aagactgacc agtcatagca ttccccagga ataagatatg ataattgagc gcgtgaatat
    11041 tacgctcact atcatttctt gggaataatt attcagctct tttgcgtaat tctgaagagc
    11101 ttaaaatcgt cacgccttca tgctcaggcc gcatcgcctc tgccaacatg acccgcgcca
    11161 cgtctctcgc atcaatggat ttccagttac ctggtaacaa acggaacagc ggcgcaaaaa
    11221 gcgtttcgtt catccgctgt ttgctacgat cgcccagtaa catcgacggg cgagcaatgg
    11281 tcaatttcgg ccagttctgg gcgattaatg cttcttccat ctcccctttg acgcggttat
    11341 agaaaaacgg cgagtgggca ttggcaccca tcgcactgac taccaacata tgctgcgcac
    11401 cgaggcgccg cccggttaat gcggtatcca ccaccagcgt gtaatcggca tgaataaacg
    11461 cctctttgct ccccgcttct cgccgcgtgg tgccgagaca acaaaacaca atgtcgatgg
    11521 gatcggtgac ctgcgccagc gcgtcggtca gttgcggatc atggggatta aacacccctg
    11581 gcatatcgcc caacggacgt cgcgtcggcg cggctatggc gttaactttc ggttcgttaa
    11641 tcaacatccg cagcaggtga ccgcccacca gccccgttgc gcctgtaatc agtacctgac
    11701 tcatcttcgc tcctttacag aattgtctgc cttgcgctcc acggctcatg caccaggctt
    11761 aatagaccat gaggtaatta tccccgattg tgggaaattc gcctcatcca atgcaacatc
    11821 gcggaggaag catgagtaag aaaattgccg ttttaatcag tgatgaattt gaggattcag
    11881 aatttacttc acccgcagac gagttccgta aagccggaca cgaagtgatt accattgaaa
    11941 aacaagcggg taaaacggtg aaaggcaaaa aaggagaagc cagcgtggcc atcgataaat
    12001 ccatcgatga agtgacgccc gcagagttcg atgccctgct gctaccgggc ggccattcac
    12061 cggattatct gcgtggcgac aaccgttttg tcaccttcac ccgcgatttt gtgaatagcg
    12121 gcaaacctgt tttcgccatc tgtcacggtc cgcagctgct gatcagcgcc gatgtgattc
    12181 gggggcgcaa actgactgcc gttaaaccga tcattattga tgttaaaaac gcgggcgcgg
    12241 aattttacga tcaggaagtg gtggtcgata aagatcagct ggttaccagc cgtacaccgg
    12301 acgatctgcc agcgtttaac cgcgaggcat tacgcctgct cggtgcctga gttacgtagc
    12361 cagatcattt ttttaccaaa gcccagcgtg ttgtcggtga atttgatttc atcaagccgg
    12421 atttcccaca ccggtgccga cagcattctg gcaaccggaa agcgacgatt gtacgcctgg
    12481 cgcgcacgct cgctttcctc accttcaagc ctgcggatct cgcctttaaa ttgcacgccg
    12541 cgaattaacg ctaccgtttt cggctgaccg tttaccgttc cggcaactgc cgcctgcggc
    12601 ccactcatct gcgcgtggcg cgttttttct tccgtcaaaa tgtagaaggc gactttctgc
    12661 gcatcaaaaa gataaaaggc attagcgcac cacagttccc cttcctgctg cacacaccag
    12721 gtgacaacat gttgttttgc cagccaacgg ctgatggcgg tgagtgtttc cattgctgtt
    12781 ctcttttata ctgtgggcct gaccttaaca tgcgacgcag acgatgacac cctggtttct
    12841 ttacttgatc cgtaccgccg acaataagct ttataccggg atcaccacag atgtcgaacg
    12901 ccgctatcag cagcaccaaa gcggcaaagg ggcgaaagca ctgcgcggga aaggagaact
    12961 gacgctggcg ttttccgcgc cagtgggcga tcgttcgctg gcactacggg cggaatatcg
    13021 cgttaagcaa ctgaccaaac ggcaaaaaga gcgtctggta gcggaaggcg cagggtttgc
    13081 agaactgttg agcagcctgc aaaccccaaa gattaaaagc gattgaagtg ctcgtgatac
    13141 tcaaccagac cagttacacc gttcagcgcg tcatccgcta aacgatgtac ctggaaggca
    13201 ctttcagtgc ccggccagcg gcaacgcaga tcgtgatgtg ccgccagttc aaagccgaaa
    13261 cggctgtaca gcgccggatc gcccagcgtc accactgcgg catagccgaa ctcattaagc
    13321 gaatcgagtc cttcatagac cagttggcgt gccagcccct gtccacggta tttttcatct
    13381 accgccagcg gtgccatgcc gacccattgc aggtcttcgc cctgcacatc aaccggacta
    13441 aatgccacat agccaatgac ctgaccttcg tcatctgtcg ccaccagccc cagcgtcaga
    13501 aagccatctt cacgcagatc gtgaaccagc ttcgcttccg catcactttc gaatgaacga
    13561 cgcagcaggg catcaatacc cggcgcatca atgggaattt ctactcgaat tagcatggtt
    13621 cacctaccga tgtctgtttg gtttcaggcg cggttttcat tcccgcctca acaaaatccg
    13681 ccagttgcag cagcatcatg cgtaaggctt tcggcatttg ctccagttca atggcgtcca
    13741 tcaggttttt cacatataac cccagctccg tatcgccttc aatcaccagc cgacgttgga
    13801 agaagagcgt atccggatct tgtttacgcg ccgcaatcat cagcagatcg ctggcgtcgg
    13861 cactaaaact cacatcagct tgcgcgttct ggctaacgac cagtttgcca ttcaccaccg
    13921 aggtaaacca ttgcaggtca atatcacgca catgaatact taaccagcgg ccttcaagaa
    13981 actccagctc gccatcatcc aacgcctggc ggaattgcca gcttaagacc tgctcaagaa
    14041 cctggcgttt tagcgcaaat ggcgtcagtt ttaccggtac actcaacaga gatggcccca
    14101 aatgcacaat acgggaacgc agtttatcca acacgagttt tactccctgt ttcaacaatc
    14161 atcctatttt gccatatcag aaaaataaca tagcggtata aatcaacaat tccatatgaa
    14221 attgctgcta ccaccaatac aactttaact gccttaaatc aaaaattgtc gcagcaaggt
    14281 taactaaaat cccagttcgt taacattttt gcgctttgat agcgcaacct tcaggaaaaa
    14341 ttatggagct gctctgccct gccggaaatc tcccggcgct taaggcggcc atcgaaaacg
    14401 gcgcagatgc tgtttatatc gggctaaaag atgataccaa tgcccgtcac ttcgccggcc
    14461 ttaactttac cgagaaaaaa ttgcaggaag cggtgagttt tgtccatcaa catcgccgca
    14521 aacttcacat cgcgattaac acttttgcgc atccggacgg ttacgcccgc tggcagcgcg
    14581 ccgtggatat ggcggcgcag ctgggtgccg acgcgctgat cctcgccgac ctcgccatgc
    14641 tggagtacgc cgccgagcgt tacccgcata ttgagcgtca tgtgtcagtg caggcttcgg
    14701 cgaccaatga agaggcgatt aacttttatc atcgccattt tgacgttgcc cgcgtggtgc
    14761 tgccgcgcgt gttgtcgatt catcaggtga aacaactggc acgggtcaca cctgtaccac
    14821 tggaagtctt tgctttcggc agcctgtgca ttatgtcgga aggtcgttgc tatctgtcgt
    14881 cgtatctgac gggtgagtcg cccaacactg tgggtgcgtg ttctccggcc cgtttcgtgc
    14941 gctggcaaca aacgccgcag gggctggaat cccgcctgaa cgaagtgctg atcgaccgtt
    15001 atcaggacgg cgaaaacgca ggttatccga cgctgtgtaa agggcgttat ctggtggacg
    15061 gcgagcgcta tcacgcgctg gaagaaccca ccagtctcaa taccctggaa ctgctgccgg
    15121 agttaatggc agcgaatatt gcttcggtga aaattgaagg ccgccagcgt agcccggcgt
    15181 atgtcagcca ggtggcgaaa gtctggcgtc aggctatcga ccgttgtaag gccgatccgc
    15241 aaaacttcat accgcaaagc gcgtggatgg agacgctcgg gtcg
//
LOCUS       DAMZFT010000118        15267 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.118, whole genome shotgun sequence.
ACCESSION   DAMZFT010000118 DAMZFT010000000
VERSION     DAMZFT010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15267)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15267)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15267
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.118"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..858
                     /gene="anmK"
                     /locus_tag="PN977_003799"
     CDS             <1..858
                     /gene="anmK"
                     /locus_tag="PN977_003799"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016248675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HDI6043798.1"
                     /translation="NLQARDIVAIGCNGQTVWHEPTGVAPHTLQIGDNNQIVARTGIT
                     VVGDFRRRDIALGGQGAPLVPAFHHALLAHPTERRMVLNIGGIANLSLLIPGQPVGGY
                     DTGPGNMLMDAWIWRQAGKPYDKDAEWARAGKVILPLLQNMLSDPYFSQPAPKSTGRE
                     YFNYGWLERHLRHFPGVDPRDVQATLAELTAVTISEQVLLSGGCERLMVCGGGSRNPL
                     LMARLAALLPGTEVTTTDAVGISGDDMEALAFAWLAWRTLAGLPGNLPSVTGASQETV
                     LGAIFPANL"
     gene            956..1285
                     /gene="mliC"
                     /locus_tag="PN977_003800"
     CDS             956..1285
                     /gene="mliC"
                     /locus_tag="PN977_003800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416156.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-type lysozyme inhibitor"
                     /protein_id="HDI6043799.1"
                     /translation="MSMKKLLLICLPVLLTGCSVFNQLVERMQTDTLEYQCDEKPLTV
                     KLNNPRQEVSFVYDNQLLHLKQGISASGARYTDGIYVFWSKGEEATVYKRDRIVLNNC
                     QLQNPQR"
     gene            1344..2000
                     /gene="pdxH"
                     /locus_tag="PN977_003801"
     CDS             1344..2000
                     /gene="pdxH"
                     /locus_tag="PN977_003801"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HDI6043800.1"
                     /translation="MSDNDELQQIAHLRREYTKGGLRRRDLPADPLTLFERWLSQACD
                     AKLADPTAMVVATVDEHGQPYQRIVLLKHYDEKGMVFYTNLGSRKAHQIENNPRVSLL
                     FPWHTLERQVMVIGKAERLSTLEVMKYFHSRPRDSQIGAWVSKQSSRISARGILESKF
                     LELKQKFQQGEVPLPSFWGGFRVSLEQIEFWQGGEHRLHDRFLYQRENDAWKIDRLAP
                     "
     gene            2129..3403
                     /gene="tyrS"
                     /locus_tag="PN977_003802"
     CDS             2129..3403
                     /gene="tyrS"
                     /locus_tag="PN977_003802"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HDI6043801.1"
                     /translation="MASSNLIKQLQERGLVAQVTDEEALAERLAQGPIALYCGFDPTA
                     DSLHLGHLVPLLCLKRFQQAGHKPVALVGGATGLIGDPSFKAAERKLNTEETVQEWVD
                     KIRKQVAPFLDFDCGENSAIAANNYDWFGNMNVLTFLRDIGKHFSVNQMINKEAVKQR
                     LNREDQGISFTEFSYNLLQGYDFACLNKQYGVVLQIGGSDQWGNITSGIDLTRRLHQN
                     QVFGLTVPLITKADGTKFGKTEGGAVWLDPKKTSPYKFYQFWINTADADVYRFLKFFT
                     FMSIEEINALEEEDKNSGKAPRAQYVLAEQVTRLVHGEEGLQAAKRITECLFSGSLSA
                     LSEADFEQLAQDGVPMVEMEKGADLMQALVDSELQPSRGQARKTIASNAITINGEKQS
                     DPEYFFKEEDRLFGRFTLLRRGKKNYCLICWK"
     gene            3465..4325
                     /gene="pdxY"
                     /locus_tag="PN977_003803"
     CDS             3465..4325
                     /gene="pdxY"
                     /locus_tag="PN977_003803"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310372.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal kinase PdxY"
                     /protein_id="HDI6043802.1"
                     /translation="MKNILAIQSHVVYGHAGNSAAEFPMRRLGANVWPLNTVQFSNHT
                     QYGKWTGCVMPPSHLTEIVQGIAAIDKLHTCDAVLSGYLGSAEQGEHILGIVRQVKAA
                     NPQAKYFCDPVMGHPEKGCIVAPGVAEFHVRHGLPASDIIAPNLVELEILCEHPVNNV
                     EEAVLAARELIAQGPQIVLVKHLARAGYSRDRFEMLLVTADEAWHISRPLVDFGMRQP
                     VGVGDVTSGLLLVKLLQGATLQEALEHVTAAVYEIMVTTKAMQEYELQVVAAQDRIAK
                     PEHYFSATKL"
     gene            complement(4369..4974)
                     /gene="gstA"
                     /locus_tag="PN977_003804"
     CDS             complement(4369..4974)
                     /gene="gstA"
                     /locus_tag="PN977_003804"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase GstA"
                     /protein_id="HDI6043803.1"
                     /translation="MKLFYKPGACSLASHITLRESGKDFTLVSVDLMKKRLENGDDYF
                     AVNPKGQVPALQLDNGTLLTEGVAIMQYLADSVPDRQLLAPVNSISRYKTIEWLNYIA
                     TELHKGFTPLFRPDTPEEYKPTVRAQLEKKLQYVNDALKDEHWICGQRFTIADAYLFT
                     VLRWAYAVKLNLEGLEHIAAFMQRMAERPEVQDALSAEGLK"
     gene            complement(5079..6581)
                     /gene="dtpA"
                     /locus_tag="PN977_003805"
     CDS             complement(5079..6581)
                     /gene="dtpA"
                     /locus_tag="PN977_003805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpA"
                     /protein_id="HDI6043804.1"
                     /translation="MSTANQKPTESVSLNAFKQPKAFYLIFSIELWERFGYYGLQGIM
                     AVYLVKQLGMSEADSITLFSSFSALVYGLVAIGGWLGDKVLGTKRVIMLGAIVLAIGY
                     ALVAWSGHDAGIVYMGMAAIAVGNGLFKANPSSLLSTCYEKNDPRLDGAFTMYYMSVN
                     IGSFFSMIATPWLAAKYGWSVAFALSVVGLLITIVNFAFCQRWVKQYGSKPDFEPINY
                     RNLLLTIIGVVALIAIATWLLHNQEVARMALGVVAFGIVVIFGKEAFAMKGAARRKMI
                     VAFILMLEAIIFFVLYSQMPTSLNFFAIRNVEHSILGLAVEPEQYQALNPFWIIIGSP
                     ILAAIYNKMGDTLPMPTKFAIGMVMCSGAFLILPLGAKFASDAGIVSVSWLVASYGLQ
                     SIGELMISGLGLAMVAQLVPQRLMGFIMGSWFLTTAGANLIGGYVAGMMAVPDNVTDP
                     LMSLEVYGRVFLQIGVATAVIAVLMLLTAPKLHRMTQDDAADKAAKAAVA"
     gene            complement(7191..7826)
                     /gene="nth"
                     /locus_tag="PN977_003806"
     CDS             complement(7191..7826)
                     /gene="nth"
                     /locus_tag="PN977_003806"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HDI6043805.1"
                     /translation="MNKAKRLEILTRLRENNPHPTTELNFSSPFELLIAVLLSAQATD
                     VSVNKATAKLYPVANTPAAMLELGVEGVKTYIKTIGLYNSKAENIIKTCRILLEQHNG
                     EVPEDRAALEALPGVGRKTANVVLNTAFGWPTIAVDTHIFRVCNRTQFAPGKNVEQVE
                     EKLLKVVPAEFKVDCHHWLILHGRYTCIARKPRCGSCIIEDLCEYKEKVDI"
     gene            complement(7826..8521)
                     /gene="rsxE"
                     /locus_tag="PN977_003807"
     CDS             complement(7826..8521)
                     /gene="rsxE"
                     /locus_tag="PN977_003807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxE"
                     /protein_id="HDI6043806.1"
                     /translation="MSEIKDVIVQGLWKNNSALVQLLGLCPLLAVTSTATNALGLGLA
                     TTLVLTLTNLTISTLRHWTPAEIRIPIYVMIIASVVSAVQMLINAYAFGLYQSLGIFI
                     PLIVTNCIVVGRAEAFAAKKGPALSALDGFSIGMGATCAMCVLGSLREIIGNGTLFDG
                     ADALLGSWAKVLRVEIFHTDSPFLLAMLPPGAFIGLGLMLAGKYLIDERMKKRRTEAA
                     AERALPNGETGNV"
     gene            complement(8525..9145)
                     /gene="rsxG"
                     /locus_tag="PN977_003808"
     CDS             complement(8525..9145)
                     /gene="rsxG"
                     /locus_tag="PN977_003808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxG"
                     /protein_id="HDI6043807.1"
                     /translation="MLKTIRKHGITLALFAAGSTGLTAAINQMTKTTIAEQASLQQKA
                     LFDQVLPAERYNNALAQSCYLVTAPELGKGEHRVYIAKQDDKPVAAVLETTAPDGYSG
                     AIQLLVGADFNGTVLGTRVTEHHETPGLGDKIELRLSDWITHFAGKKISGADDAHWAV
                     KKDGGDFDQFTGATITPRAVVNAVKRAGLYAQTLPSQLSQLPACGE"
     gene            complement(9149..10207)
                     /gene="rsxD"
                     /locus_tag="PN977_003809"
     CDS             complement(9149..10207)
                     /gene="rsxD"
                     /locus_tag="PN977_003809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxD"
                     /protein_id="HDI6043808.1"
                     /translation="MVFRIASSPYTHNQRQTSRIMLLVLLAAVPGIAAQLWFFGWGTL
                     VQILLASVSALLAEALVLKLRKQSVAATLKDNSALLTGLLLAVSIPPLAPWWMVVLGT
                     VFAVIIAKQLYGGLGQNPFNPAMIGYVVLLISFPVQMTSWLPPHEIAVNIPGFIDAIQ
                     VIFSGHITSGGDMNTLRLGIDGISQATPLDTFKTSVRAGHSVEQIMQYPIYSGMLAGV
                     GWQWVNLAWLAGGVWLLWQKAIRWHIPLSFLVTLALCATLGWLFSPETLAAPQIHLLS
                     GATMLGAFFILTDPVTASTTNRGRLIFGALAGLLVWLIRSFGGYPDGVAFAVLLANIT
                     VPLIDYYTRPRVYGHRKG"
     gene            complement(10208..12238)
                     /gene="rsxC"
                     /locus_tag="PN977_003810"
     CDS             complement(10208..12238)
                     /gene="rsxC"
                     /locus_tag="PN977_003810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HDI6043809.1"
                     /translation="MLKLFSAFRKNKIWDFNGGIHPPEMKTQSNGTPLRQVPLAQRFV
                     IPLKQHIGAEGELCVSVGDKVLRGQPLTRGRGKMLPVHAPTSGTVTAIAPHSTAHPSA
                     LAELSVIIDADGEDCWIPRDGWDDYRSRSREELIERIHQFGVAGLGGAGFPTGVKLQG
                     GGDKIETLIINAAECEPYITADDRLMQDCAAQVVEGIRILAHILQPREILIGIEDNKP
                     QAISMLRAVLADSHDISLRVIPTKYPSGGAKQLTYILTGKQVPHGGRSSDIGVLMQNV
                     GTAYAVKRAVIDGEPITERVVTLTGEAIARPGNVWARLGTPVRHLLNDAGFCPSADQM
                     VIMGGPLMGFTLPWLDVPVVKITNCLLAPSANELGEPQEEQNCIRCSACADACPADLL
                     PQQLYWFSKGQQHDKATTHNIADCIECGACAWVCPSNIPLVQYFRQEKAEIAAIRQEE
                     KRAAEAKARFEARQARLEREKAARLERHKSAAVQPAAKDKNAIAAALARVKEKQAQAT
                     QPIVIKAGERPDNSAIIAAREARKAQARAKQAELQQTNDAATVADPRKTAVEAAIARA
                     KARKLEQQQANAEPEQQVDPRKAAVEAAIARAKARKLEQQQANAEPEQQVDPRKAAVE
                     AAIARAKARKLEQQQANAEPEEQVDPRKAAVAAAIARVQAKKAAQQKVVNED"
     gene            complement(12231..12809)
                     /gene="rsxB"
                     /locus_tag="PN977_003811"
     CDS             complement(12231..12809)
                     /gene="rsxB"
                     /locus_tag="PN977_003811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxB"
                     /protein_id="HDI6043810.1"
                     /translation="MNAIWIAVAAVSLLGLAFGAILGYASRRFAVEDDPVVEKIDEIL
                     PQSQCGQCGYPGCRPYAEAISCNGEKINRCAPGGEAVMLKIAELLNVEPQPLDGEAQE
                     LTPARMVAVIDENNCIGCTKCIQACPVDAIVGATRAMHTVMSDLCTGCNLCVDPCPTH
                     CISLQPVAETPDSWKWDLNTIPVRIIPVEHHA"
     gene            complement(12809..13390)
                     /gene="rsxA"
                     /locus_tag="PN977_003812"
     CDS             complement(12809..13390)
                     /gene="rsxA"
                     /locus_tag="PN977_003812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017456621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HDI6043811.1"
                     /translation="MTDYLLLFVGTVLVNNFVLVKFLGLCPFMGVSKKLETAMGMGLA
                     TTFVMTLASICAWLIDTWILIPLNLIYLRTLAFILVIAVVVQFTEMVVRKTSPVLYRL
                     LGIFLPLITTNCAVLGVALLNINLGHNFLQSALYGFSAAVGFSLVMVLFAAIRERLAV
                     ADVPAPFRGNAIALITAGLMSLAFMGFSGLVKL"
     gene            complement(13467..13907)
                     /locus_tag="PN977_003813"
     CDS             complement(13467..13907)
                     /locus_tag="PN977_003813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707526.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2569 domain-containing protein"
                     /protein_id="HDI6043812.1"
                     /translation="MTTTTPQRIGGWLLGPLAWLLVALLSTTLALLLYTAALSSPQTF
                     QTLGGQALTTQILWGVSFITAIAMWYYTLWLTIAFFKRRRCVPKHYIIWLLISVLLAV
                     KAFAFSPVEDGIAVRQLLFTLLATALIVPYFKRSSRVKATFVNP"
     gene            complement(13993..14208)
                     /gene="ydgT"
                     /locus_tag="PN977_003814"
     CDS             complement(13993..14208)
                     /gene="ydgT"
                     /locus_tag="PN977_003814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription modulator YdgT"
                     /protein_id="HDI6043813.1"
                     /translation="MTVQDYLLKFRKISSLESLEKLYDHLNYTLTDDQELINMYRAAD
                     HRRAELVSGGRLFDLGQVPKSVWHYVQ"
     gene            complement(14481..14606)
                     /gene="blr"
                     /locus_tag="PN977_003815"
     CDS             complement(14481..14606)
                     /gene="blr"
                     /locus_tag="PN977_003815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division septum protein Blr"
                     /protein_id="HDI6043814.1"
                     /translation="MNRLIELTGWIVLVVSVILLGVASHIDNYQPPEQSTTVQHK"
     gene            14849..>15267
                     /locus_tag="PN977_003816"
     CDS             14849..>15267
                     /locus_tag="PN977_003816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310359.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HDI6043815.1"
                     /translation="MSDNIRVGLIGYGYASKTFHAPLIAGTPGLELAVISSSDETKVK
                     ADWPTVAVVSEPKHLFNDPNIDLIVIPTPNDTHFPLAKAALEAGKHVVVDKPFTVTLS
                     QARELEALAKSLGRVLSVFHNRRWDSDFLTLKGLLVEG"
BASE COUNT         3882 a         3863 c         3905 g         3617 t
ORIGIN      
        1 aacctgcagg cgagagatat cgttgcgatc ggctgtaacg gtcaaaccgt ctggcatgaa
       61 ccgacgggcg tggcaccaca cactttacag attggcgata acaatcaaat tgtggcacgc
      121 accggaatta cggttgtcgg tgattttcgc cgtcgcgata ttgccttggg aggacaaggc
      181 gcaccgctgg tacctgcgtt ccatcatgcg ctgctggctc acccaaccga gcgacgaatg
      241 gtgctcaata ttggtggcat tgccaatctg tcactgctca ttcctgggca gccggtaggg
      301 ggctacgata ccggccctgg taacatgctg atggatgcct ggatctggcg tcaggcaggt
      361 aaaccttacg ataaagatgc cgagtgggca cgggcgggta aagtcattct cccactgctg
      421 caaaatatgc tcagcgaccc atatttctcg caacctgcac cgaaaagtac cggacgcgaa
      481 tactttaact acggctggct ggagcgccat ttgcgccatt ttccgggtgt tgatccccga
      541 gatgtacagg cgacactggc agaactcacc gccgtgacca tttctgaaca agttttgttg
      601 agcggtggct gcgaacgatt gatggtatgt ggtggaggaa gtcgtaatcc gctactcatg
      661 gcgcgtctgg cggcattact gccaggcaca gaagtcacca ccaccgatgc cgttggcatt
      721 agtggcgatg acatggaagc attggctttc gcctggcttg cctggcgaac gctggcggga
      781 ttaccaggaa atctgccttc cgtcactggc gcaagccagg agacggtact gggggctatt
      841 ttccccgcta acctgtgata tcagagttaa ctgaattttt cttaccgcgt ctgccgttac
      901 acttggaacg ttagggaggg cgtattgccc tccagaccag gaaagtcttc gggatatgtc
      961 tatgaaaaaa ctgttactga tctgtttgcc ggtccttctc accggctgta gcgtgttcaa
     1021 tcaactggtt gagcgcatgc aaaccgatac gctggaatac cagtgtgatg aaaaaccgtt
     1081 gacggtcaaa ctgaataatc cgcgccagga agtcagtttt gtttatgata atcaactatt
     1141 gcatctcaaa cagggcattt ctgcgtctgg tgcacgttac accgacggga tctatgtttt
     1201 ctggtcgaaa ggcgaggaag cgactgtcta taaacgcgac cgcatcgtct tgaataactg
     1261 tcagttacaa aatccacagc gttgagattt ttccaggggc ggcgcacaat agcgtcaccc
     1321 actgacaatc cgtaaagaaa accatgtctg ataacgacga attgcagcaa atcgcgcatc
     1381 tgcgccgtga atacaccaaa ggcgggttac gccgccgcga tcttcccgcc gatccattaa
     1441 ccctttttga acgctggctc tctcaggctt gtgacgccaa actggcggac cctaccgcga
     1501 tggtggtcgc taccgtggat gaacatggtc agccttatca gcgcatcgtt ttactcaaac
     1561 attacgacga aaaaggcatg gtgttttaca ccaacctcgg cagccgtaaa gcacatcaaa
     1621 tcgaaaataa tccgcgcgtt agcctgctgt tcccgtggca tacccttgag cgccaggtga
     1681 tggtgatcgg taaagcggaa cgactttcga ctctcgaagt gatgaaatat tttcatagtc
     1741 gcccacgtga tagccagatt ggtgcatggg tttcgaagca gtccagtcgc atttctgccc
     1801 gcggtatcct tgaaagtaaa ttcctggagc tgaagcagaa gtttcaacag ggcgaagtgc
     1861 cattgccgag tttttggggc ggttttcgcg tcagccttga acagattgag ttctggcagg
     1921 gcggtgagca tcgcctgcat gaccgctttt tgtaccagcg tgaaaatgat gcgtggaaga
     1981 ttgatcgtct tgccccctga aaagatgcaa aaatcttgct ttaatcgctg gtactcctga
     2041 ttctggcact ttattctatg tctctttcgc atctggcgaa aagtcgtgta ccggcaaagg
     2101 tgcagtcgtt atatacatgg agattttgat ggcaagcagt aacttgatta aacaattgca
     2161 agagcggggg ctggtagccc aggtgacgga cgaggaagcg ttagcagagc gactggcgca
     2221 aggcccgatc gcgctctatt gcggcttcga tcctaccgct gacagcttgc atttggggca
     2281 tcttgttcca ttgttatgcc tgaaacgctt ccagcaggcg ggccacaagc cggttgcgct
     2341 ggtaggcggc gcgacgggtc tgattggcga cccgagcttc aaagctgctg agcgtaagct
     2401 gaacaccgaa gaaactgttc aggaatgggt ggacaaaatc cgcaagcagg ttgccccgtt
     2461 cctcgatttc gactgtggag aaaactctgc tatcgcggcg aacaactatg actggttcgg
     2521 caatatgaat gtgctgacct tcctgcgcga tattggcaaa cacttctccg ttaaccagat
     2581 gatcaacaaa gaagcggtta agcagcgtct caaccgtgaa gatcagggga tttcgttcac
     2641 tgagttttcc tacaacctgt tgcagggtta tgacttcgcc tgtctgaaca aacagtacgg
     2701 tgtggtgctg caaattggtg gttctgacca gtggggtaac atcacttctg gtatcgacct
     2761 gacccgtcgt ctgcatcaga atcaggtgtt tggcttgacc gttccgctga tcaccaaagc
     2821 agacggcacc aaatttggta aaactgaagg cggcgcagtc tggttggatc cgaagaaaac
     2881 cagcccgtac aaattctacc agttctggat taacactgcg gatgccgacg tttaccgctt
     2941 cctgaagttc ttcaccttta tgagcattga agagatcaac gccctggaag aagaagataa
     3001 aaacagcggt aaagcaccgc gcgcccagta tgtactggcg gagcaggtga ctcgtctggt
     3061 tcacggtgaa gaaggtttac aggcggcaaa acgtattacc gaatgcctgt tcagcggttc
     3121 cttgagtgcg ctgagtgaag cggacttcga acagctggcg caggacggcg taccgatggt
     3181 tgagatggaa aaaggcgcag acctgatgca ggcgctggtc gattctgaac tgcaaccatc
     3241 tcgtggtcag gcgcgtaaaa ctatcgcctc caatgccatc accattaacg gtgaaaaaca
     3301 atccgatcct gaatacttct ttaaagaaga agatcgtctg tttggtcgtt ttaccttact
     3361 gcgtcgcggc aaaaagaatt actgtctgat ttgctggaaa taatgcatta agtggaaagg
     3421 gggagtgaga aatcactccc cctggttttt atacagggaa catgatgaaa aatattctcg
     3481 ctatccagtc tcacgttgtt tatggtcatg cgggtaacag tgcggcagag tttccgatgc
     3541 gccgcctggg cgcgaacgtc tggccgctga acaccgttca attttctaat cacacccaat
     3601 acggcaaatg gaccggctgc gtgatgccgc ccagccattt aaccgaaata gtgcaaggca
     3661 ttgccgccat tgataaatta cacacctgtg atgccgtatt aagtggctat ctgggatcgg
     3721 cggagcaggg tgaacatatc ctcggtatcg tccgtcaggt gaaagccgcg aatccgcagg
     3781 cgaaatattt ttgcgatccg gtaatgggtc atccggaaaa aggctgtatc gttgcaccgg
     3841 gtgtcgcaga gtttcatgtg cgtcacggtt tgcctgccag cgatatcatt gcgccgaatc
     3901 tggttgagct ggagatactc tgtgagcatc cggtaaataa cgtcgaagaa gcggttctgg
     3961 cagcgcgtga actcattgcg caagggccac aaattgtgtt ggttaaacac ctggcgcggg
     4021 ctggctacag ccgtgaccgt tttgaaatgc tgctggttac cgccgatgaa gcctggcata
     4081 tcagccgtcc gctggtggat tttggtatgc gccagccggt aggtgttggt gatgtgacga
     4141 gcggtttact gctggtgaaa ctgcttcagg gggcaacgct gcaggaggcc ctggaacatg
     4201 tgaccgctgc agtatacgaa atcatggtga ccaccaaagc aatgcaggaa tatgagttgc
     4261 aagtggttgc tgctcaggat cgtattgcca aaccagaaca ttatttcagc gcaacaaagc
     4321 tctgaaatat ctcatttcgg cccacaacga cctgtgggcc gatggctatt actttaagcc
     4381 ttccgctgac agcgcgtctt gtacttccgg acgttcagcc atacgttgca taaatgccgc
     4441 aatgtgctct aacccttcca gattcagttt caccgcgtat gcccagcgca gaaccgtaaa
     4501 cagataggca tcagcaattg taaatcgttg cccgcagatc cagtgctcat ccttcagagc
     4561 atcgttcaca tattgcagct tcttctccag ctgcgcgcga actgttggtt tgtactcttc
     4621 cggtgtatca gggcgaaaca gaggtgtgaa gcctttatgc agctcggtgg cgatgtaatt
     4681 cagccattcg atggttttat agcgggaaat actgtttacc ggtgccagca actggcggtc
     4741 ggggacgctg tcggcaagat actgcataat tgctacgcct tccgtcagca aagtaccatt
     4801 gtccagctgc aatgcaggca cctgtccctt agggttaacg gcaaagtaat cgtcaccgtt
     4861 ttcgagacgt tttttcatta aatccacact gacgagcgta aaatcctttc cgctctcacg
     4921 cagggtgata tgggaagcga gagagcaggc acccggtttg tagaacaatt tcatcggtaa
     4981 ctccttttgt ctgtggttta aagtatggta gtgcgttgct gaacaaaaaa aagccgctaa
     5041 cgaaaagtta gcggcttgta aaagagtttc cctgaatttt acgctacagc agctttcgcc
     5101 gctttgtctg cagcgtcatc ctgcgtcatg cggtgcagtt tcggcgcggt cagcagcatc
     5161 agtactgcaa taacggcagt agcgacacca atctgcaaga atacgcgacc atagacttcc
     5221 agtgacatca gcggatcggt aacgttatcc ggcacagcca tcatacccgc aacataacca
     5281 ccaatcaggt ttgcaccggc agtggtcagg aaccagctac ccataatgaa gcccatcaga
     5341 cgctgcggaa cgagttgtgc gaccattgcc agacccagac cagagatcat cagttccccg
     5401 atactctgca ggccatagct cgcgaccagc cagcttacag acacgatacc agcgtcagac
     5461 gcgaatttcg cacccagcgg cagaatcagg aacgcaccag aacacatcac catgccgatt
     5521 gcaaacttgg ttggcatcgg cagggtatcg cccatcttgt tatagatagc ggccagaatc
     5581 ggactaccga tgatgatcca gaacgggttc agtgcctgat actgctcagg ttctacggcc
     5641 agacccagaa tggagtgctc aacgttacga atcgcaaaga agttcagtga cgttggcatc
     5701 tggctgtaca gcacgaagaa gataatggct tcgagcatca ggatgaaggc aacgatcatt
     5761 ttacgacgcg cagcaccttt catcgcgaag gcttctttac cgaagataac cacaataccg
     5821 aaggcaacaa cgcccagcgc catacgcgca acttcctgat tgtgcagcag ccaggtggcg
     5881 atagcgatca gtgccacaac accaataatg gtcagcagca ggttacggta gttgataggc
     5941 tcgaagtctg gttttgaacc gtactgttta acccagcgtt ggcagaaggc gaagttaacg
     6001 atagtgatca gcaggcctac aacgctcaac gcaaacgcaa cactccagcc gtatttcgcg
     6061 gccagccacg gggtggcgat catagagaag aaagagccga tgttgacgga catgtagtac
     6121 atggtgaatg caccgtccag acgcgggtcg tttttctcat agcatgtaga aagcagagaa
     6181 gacgggttag ctttaaacag gccgttaccg accgcaatag ccgccatacc catataaacg
     6241 ataccggcgt cgtgaccaga ccaggcaacc agagcataac caatcgccag cacaatagcg
     6301 ccaagcataa ttacgcgttt agtacccagt accttgtcac ctaaccagcc gccgatagcg
     6361 accagaccat aaaccagggc actaaaggaa gagaaaaggg tgattgaatc cgcttcagac
     6421 atacccagtt gtttaaccag gtaaacagcc ataattcctt gtaggccgta ataaccaaaa
     6481 cgttcccata actcaatcga gaagatgaga tagaacgcct tcggttgttt gaaagcgttc
     6541 aaactgacgc tttcagttgg tttttggttt gcagtggaca cttttacctc tttttttaca
     6601 tcccatatta acgggggtgc tttctcgcgt tatgcctaaa aacatacgcg caattgttat
     6661 agtgggaggg gaaacggcag gtaatgttca ctatcttgca ctttcagaca agtcctttgt
     6721 aataatctgt tacatataac tgaggcgggg aattcaacca ttccgggaaa tgttatccag
     6781 cgttaaattt ttcaaaaaac gtaacggcag attttttcac taccctgaga gggggatgat
     6841 ggcttaacgg cgatatgttc tgctatttag cgtttttcct agtgagatag tccagtttct
     6901 gaaaaatagc cagtgtaatg ttttgtaggt caatatttgt tgcgtgtgta aatttaaatg
     6961 attttaacct agttcttgca atgtttttac attgccacag catcgtgatc ttgatcacga
     7021 atgaatagaa aatttcgcta ttaaaaaaac aattaacctg catttttggt tatccacaaa
     7081 cggattacct gatgataacg ctaagcaatg gcatcattga cgttaaaaag atgttttaaa
     7141 agaggataaa gaaaggttat gaatggggta atcggtgtta ccccttttct tcagatgtca
     7201 actttctctt tgtattcaca aagatcttca ataatacaag agccacagcg gggcttgcgg
     7261 gcaatgcagg tatatcgccc gtggaggatc aaccaatggt ggcagtcgac tttaaactct
     7321 gccggaacca ctttcagtag cttttcttct acctgttcga cgtttttccc cggcgcaaat
     7381 tgagtacgat tacaaacgcg gaaaatgtgc gtgtcgacag caatagtcgg ccagccgaat
     7441 gcagtgttta atacgacgtt ggctgtttta cgacctacac caggcagggc ttcaagcgca
     7501 gcacgatctt ccggaacctc gccattatgc tgttccagta agatacggca ggttttgatg
     7561 atattttctg ctttgctgtt ataaagccca atggttttga tataggtttt caccccttcg
     7621 acgcccagtt caagcatcgc tgcaggtgta ttagccaccg ggtagagttt cgccgtcgcc
     7681 ttattaacac tgacatcggt cgcctgagcg gaaagcagta cggcaatcag caattcaaaa
     7741 ggcgaactga aattaagctc ggtggtggga tgaggattgt tctcacgcag gcgagtgagg
     7801 atctccaggc gttttgcttt gttcatcaga cattccctgt ttcaccgttt ggcaatgcac
     7861 gttcggcagc tgcttcagta cggcgctttt tcattctttc atcaatcagg tatttccctg
     7921 ccagcatcag tcccaggcca ataaatgcac ctggtggcag catcgccagc aggaaagggg
     7981 agtcggtgtg gaaaatttcc acgcgtaata cttttgccca gctacctaac agcgcatctg
     8041 caccgtcaaa caacgtgcca ttgccgataa tttcgcgtag tgaacccagc acgcacatgg
     8101 cgcaggttgc gcccatacca attgaaaagc cgtccagtgc cgaaagcgcc ggaccttttt
     8161 tggcggcgaa ggcttcagcg cggcccacaa cgatacagtt agtgacaatc agcggaataa
     8221 aaatccctaa tgattgatac aggccaaagg cgtaggcgtt gatcagcatc tgtacagcgc
     8281 tgaccaccga ggcgatgatc atcacgtaaa tgggaatgcg gatctcggct ggcgtccagt
     8341 gacgcagcgt cgaaatggtc aagttggtca gcgtcagtac cagcgtagtc gcaagtccta
     8401 aacccagagc gttagtggca gtggacgtga ccgccaatag cggacagagg ccgagcagct
     8461 ggaccagcgc agagttgttt ttccacaacc cctgaacaat aacgtcttta atttcgctca
     8521 cgctttattc tccacaggca ggaagttgag aaagttgtga cggtaacgtc tgagcgtaca
     8581 atccggcgcg ttttaccgca ttaaccaccg cgcggggagt aatcgtcgcg ccggtgaact
     8641 ggtcgaaatc accaccatct ttcttcaccg cccagtgcgc atcatccgca ccactgattt
     8701 ttttacccgc aaaatgggtg atccagtcag aaaggcgcag ttcgatttta tcgccaagcc
     8761 ctggcgtttc gtggtgctct gtcacgcgcg tgccaagtac cgtgccgtta aaatcggctc
     8821 ccaccagcag ctgaatcgca ccggaatagc catctggcgc ggttgtttcc agaacggcgg
     8881 ctaccggttt gtcatcctgt ttggcgatgt aaacccgatg ctcaccttta cctaactctg
     8941 gcgcagtgac cagatagcaa ctctgtgcca gcgcattgtt atagcgttcg gctggcagca
     9001 cctgatcaaa taacgccttt tgttgcagac tggcctgttc agcaatcgtc gttttggtca
     9061 tctggttgat ggccgcagtt aaccctgttg aacccgctgc aaatagtgcc agtgtaatgc
     9121 cgtgttttcg gatagttttc agcatggttt accctttgcg atggccgtag acgcgcggac
     9181 gcgtgtagta atcgatcaga ggaaccgtga tgttcgccag caggacggca aaagccacgc
     9241 cgtcaggata gccgccgaaa ctgcggatca accagactaa taagcccgcc agcgcgccga
     9301 aaataagacg accacgattg gtcgtagaag cggtaaccgg gtcagtcaaa ataaagaatg
     9361 cgccgagcat ggtcgctcca gacagcagat gaatttgcgg tgctgccagt gtttctggtg
     9421 agaacaacca gcccaacgtt gcgcataacg ccagcgttac taagaagctg aggggaatat
     9481 gccagcgaat cgctttctgc cacagcaacc atacgccgcc agccagccag gcgagattta
     9541 cccattgcca accaacgccc gccagcatac cgctgtagat cggatattgc ataatctgtt
     9601 caaccgaatg accggcacgg acagaggttt taaatgtatc cagcggtgtc gcctgactaa
     9661 tgccatcaat acctaagcgt agcgtgttca tatcaccacc actggtgata tgtccgctga
     9721 aaataacctg tatggcgtcg ataaaaccag ggatgttgac cgcaatttca tgtggcggta
     9781 accagctggt catctgcacc gggaaggaga tcagtaagac cacataacca atcattgccg
     9841 gattaaacgg gttttgcccc agaccgccat acaattgttt agcgatgatc accgcaaaca
     9901 ccgtacccag cacgaccatc caccatggcg cgaggggggg aatacttacc gccagcaata
     9961 agcctgtcag caatgctgag ttatctttca acgttgcggc taccgactgc ttgcgtagtt
    10021 tgagtacgag agcttcggct aacagagcac tgaccgacgc caacaggatc tgaacgagag
    10081 taccccaacc aaaaaaccac agttgcgctg cgattcctgg cacggctgcg agcaacacca
    10141 acagcataat gcgcgatgtc tggcgctggt tatgggtata aggggagcta gctattctga
    10201 ataccattta gtcctcgttt acaacctttt gctgggcggc ttttttggcc tgaacgcggg
    10261 caatagccgc ggcaactgcc gctttgcgcg gatcaacctg ttcttctggc tccgcattag
    10321 cctgttgctg ttccagtttg cgcgctttgg cacgggcaat agcggcttcg acggcagctt
    10381 tgcgcggatc gacctgttgt tctggttccg cattagcctg ttgctgttcc agcttgcgtg
    10441 ctttggcacg ggcaatagcg gcttcgacgg cggctttgcg cggatcgacc tgttgttctg
    10501 gttccgcatt agcctgttgc tgttccagct tgcgcgcttt ggcgcgggcg atagccgctt
    10561 caactgcagt tttacgtgga tcagcaacgg ttgcagcgtc gttagtttgc tgcagttctg
    10621 cctgtttcgc tctggcttgc gctttacggg cttcccgtgc tgcaataatt gcactgttat
    10681 ccgggcgttc gcccgcttta atcacaatag gctgtgtagc ctgggcctgt ttctctttca
    10741 cccgcgccag agcggcagca atcgcatttt tatctttggc tgcaggttga acggctgcgc
    10801 tcttatgtcg ttcaaggcga gccgcttttt cgcgctccag acgagcctgg cgcgcttcga
    10861 aacgcgcttt ggcttctgcg gcgcgctttt cttcctgacg aatagccgca atttcagctt
    10921 tttcctggcg gaaatattgc accaggggaa tattgctcgg gcaaacccac gcgcaagctc
    10981 cacattcaat gcagtcggca atgttatgcg tggtggcttt atcgtgttgc tgacctttgc
    11041 tgaaccagta tagctgttgc ggcaaaagat ccgcagggca ggcgtcagca caggcactac
    11101 aacggatgca attttgttct tcctgtggtt cgccaagctc attggcagag ggagccaaca
    11161 gacagttggt aatctttacg accgggacat ccagccatgg caaggtaaag cccattaacg
    11221 ggccgcccat aatcaccatt tgatcggcag aggggcagaa tccggcgtca ttcaataaat
    11281 gacgcacggg cgtccccagc cgtgcccaga cgttgcccgg gcgagcgatt gcttcgccag
    11341 tcagggttac gacacgctcg gtaatcggct cgccatcaat aacggcacgt ttcactgcat
    11401 aagcagtgcc gacgttttgc attaatacgc cgatatcgga tgaacgcccg ccatgtggaa
    11461 cctgcttccc ggtcagaatg taggttaatt gtttagcacc gccagaagga tatttggttg
    11521 gaatcacccg cagagaaata tcgtgagagt ccgccagcac cgcgcgcagc atggaaatcg
    11581 cctgcggttt gttatcttca atgccgataa gaatttcgcg tggctgcaga atatgcgcaa
    11641 gaatgcgaat accctctacg acctgagccg cgcaatcctg catcaaacgg tcatcggcgg
    11701 taatgtacgg ctcgcactca gcagcgttga taatcaacgt ttcaatctta tctccgccac
    11761 cctgcaattt aacgcctgtc gggaatcccg caccacccag cccggcaaca ccaaactgat
    11821 gaatgcgctc aattaactct tcgcgactgc gagagcgata atcgtcccag ccgtcgcgcg
    11881 ggatccagca gtcttcgcca tcggcatcaa taatcacgct taattcagct aaagctgaag
    11941 gatgagccgt agagtggggc gcaatagccg taacggtacc cgaggtgggc gcgtgaaccg
    12001 gcagcatttt gccgcgtcca cgggtaagcg gctggccgcg cagtacttta tcgccgacgc
    12061 taacgcacaa ctcaccttca gcgccaatat gttgtttcag tggtatgaca aaacgctgcg
    12121 ccagtggtac ctggcgcagg ggcgtaccgt tggactgggt tttcatctcc ggtggatgga
    12181 tgccgccgtt gaaatcccag attttatttt ttctgaatgc agagaataac ttaagcatgg
    12241 tgttccacgg gaatgatacg cacgggaatg gtgttcagat cccatttcca ggagtcaggt
    12301 gtttctgcaa ccggttgcaa cgagatgcag tgcgtcgggc acggatcaac acataaattg
    12361 cagcccgtac agagatcact cattaccgta tgcatggcgc gggtagcgcc aacgatggcg
    12421 tctaccggac acgcctgaat acatttagtg cagccaatac agttattttc atcaataacc
    12481 gccaccatcc gcgcaggcgt tagctcttgc gcttcgccat ccagcggctg tggctcgaca
    12541 ttaagcaact cagcaatttt tagcatcaca gcttcgccac ctggggcgca gcggttaatt
    12601 ttttcaccgt tacagctgat ggcttccgcg taggggcgac agccgggata accgcactga
    12661 ccacactggc tctgcggtaa gatttcgtca attttctcaa cgaccggatc gtcttccacc
    12721 gcaaaacggc gggaggcata acccagaatg gcgccaaacg ccaggcccag caggctcacg
    12781 gcggcaacgg caatccagat agcattcatt acaacttcac caaaccacta aagcccataa
    12841 aggccagaga cataagacct gcggtaatta acgcaatggc attaccgcga aaaggtgccg
    12901 ggacatcagc cacagcgagg cgttcgcgga tggcggcgaa gagcaccatc accagcgaga
    12961 aaccgacagc ggcggaaaaa ccgtacagcg ccgactgcaa gaaattgtgc ccgagattga
    13021 tattcagcaa cgccacgccg agcactgcac aattggtggt gataagcggc aaaaaaatcc
    13081 ccagcagacg gtaaagcacc gggctggttt tgcgcaccac catctcggtg aactgcacaa
    13141 ccacagcaat caccagaata aacgccaggg tgcgcaggta aatcagatta agcgggatca
    13201 aaatccacgt atcgataagc caggcgcaaa tagacgccag cgtcattaca aacgttgttg
    13261 ccagccccat gcccatcgcg gtttccagct ttttggaaac ccccataaac ggacagagac
    13321 cgagaaactt gaccagtaca aagttattga ccagtacagt tccgacaaac agtaacaggt
    13381 agtcagtcat ttttcggcct gaaataaaaa agccgcctat tatccgttaa tccagagcag
    13441 gcgacaacag gttaactgta aggttattac ggattcacaa acgtcgcttt cacccgcgac
    13501 gagcgtttga aataggggac gatcaatgcg gttgccaaca aagtaaacag caactggcga
    13561 acggctatgc catcttctac gggtgaaaaa gcgaacgctt ttactgccag caatacggaa
    13621 ataagcagcc agataatata gtgtttagga acacaacgcc gacgtttaaa gaaagcgatg
    13681 gtcaaccaca gtgtgtaata ccacatggcg atagcggtaa taaaagaaac gccccataag
    13741 atttgtgtcg tcagtgcttg tccgccgagc gtttgaaatg tttgcggaga agataacgca
    13801 gcggtataca gcaacaacgc cagcgtcgta cttaacagag cgactaaaag ccaggccaat
    13861 gggccaagca accagcctcc aatacgttgt ggcgttgtgg tggtcatagg ttctccagaa
    13921 tagcgacgca aacaggcaaa tctaatttta ggggcgttat tataaggctt ttataaccga
    13981 tcgctacttt ctttactgga catagtgcca gacggacttc ggtacctggc cgaggtcaaa
    14041 caaacgcccg ccagaaacca gctctgcgcg gcggtgatcg gcagcacgat acatattgat
    14101 cagttcctga tcatccgtca gagtgtaatt aagatggtcg tagagttttt ccagactttc
    14161 gagtgaactg attttgcgaa attttaataa gtagtcctga acagtcataa aaggtccata
    14221 aaataattaa taccaaagga gtaggtatta ttagtgatga taaaatttca ccaaaagtga
    14281 acgcgtgtgt tattacaata ataagaatgc gctgatggca aatattcaga gatgaaaaac
    14341 tgctctgaaa agagtaacgc gctttactat ccctgacaat caccaacaac atcgaacaag
    14401 ataataaatt cctggtttaa tatccgacaa gtgaaaacat gcacccggac gggcagcatg
    14461 tcgctctaca agcgcagagc ttacttgtgt tgtaccgtag tactctgttc aggtggctga
    14521 tagttgtcaa tgtgactcgc cacgccaaga agaatgactg aaacgacaag aacgatccaa
    14581 cctgttaatt caataagacg attcattaca gcccacatct ctgttgattg atccattaac
    14641 ttcagggggt aaatgttact tagcaataat agctccagca agatttttac tgaggttttt
    14701 gcgatattta gcttttgtcg ttggaaaatt cgctattttt ttgacttaag ttaaacaaca
    14761 tcccttattg ctggcaggtt attaaactgt tgagcgaggg taaaggatag tgtcaaatag
    14821 ccatcatact tcaatgagag gcaatgacat gagcgacaac atccgtgttg ggttgattgg
    14881 gtatggttat gcgagcaaaa ccttccatgc gcccctgatt gcgggcacgc ccgggctgga
    14941 actggcggta atctccagca gcgatgaaac aaaagtaaaa gccgactggc caacggttgc
    15001 ggttgtctct gagccgaagc atctgtttaa cgatcccaac atagacctga ttgtcattcc
    15061 tacacccaac gatacccatt tcccgttagc caaagcggcg cttgaggcgg gtaaacatgt
    15121 ggtcgttgat aaacccttta ccgtgacact gtcacaagcg cgagagctgg aagcgctggc
    15181 aaaaagcctg gggcgtgtgc tgtctgtatt ccataaccgt cgctgggata gcgatttcct
    15241 gacgctaaaa ggtttgctcg tggaagg
//
LOCUS       DAMZFT010000119        14875 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.119, whole genome shotgun sequence.
ACCESSION   DAMZFT010000119 DAMZFT010000000
VERSION     DAMZFT010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14875)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14875)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14875
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.119"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(16..1926)
                     /locus_tag="PN977_003817"
     CDS             complement(16..1926)
                     /locus_tag="PN977_003817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HDI6043816.1"
                     /translation="MTDDFAPDGQLAKAIPGFKPREPQRQMAVAVTQAIEKGQPLVVE
                     AGTGTGKTYAYLAPALRAKKKVIISTGSKALQDQLYSRDLPTVSKALKYTGNLALLKG
                     RSNYLCLERLEQQALAGGDLPVQILSDVILLRSWSNQTVDGDISTCVSVAEDSQAWPL
                     VTSTNDNCLGSDCPMYKDCFVVKARKKAMDADVVVVNHHLFLADMVVKESGFGELIPE
                     ADVMIFDEAHQLPDIASQYFGQSLSSRQLLDLAKDITIAYRTELKDTQQLQKCADRLA
                     QSAQDFRLQLGEPGYRGNLRELLANPQIQRAFLLLDDTLELCYDVAKLSLGRSALLDA
                     AFERATLYRTRLKRLKEINQPGYSYWYECTSRHFTLALTPLSVADKFKELMAQKPGSW
                     IFTSATLSVNDDLHHFTSRLGIEQAESLLLPSPFDYSRQALLCVPRNLPQTNQPGSAR
                     QLAAMLRPIIEANNGRCFMLCTSHAMMRDLAEQFRATMTLPVLLQGETSKGQLLQQFV
                     SAGNALLVATSSFWEGVDVRGDTLSLVIIDKLPFTSPDDPLLKARMEDCRLRGGDPFD
                     EVQLPDAVITLKQGVGRLIRDADDRGVLVICDNRLVMRPYGATFLASLPPAPRTRDIA
                     RAVRFLAIPSSR"
     gene            2058..2402
                     /locus_tag="PN977_003818"
     CDS             2058..2402
                     /locus_tag="PN977_003818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HDI6043817.1"
                     /translation="MTIVRIDAEARWSDVVIHNNTLYYTGVPENLDADAFEQTANTLA
                     QIDAVLEKQGSNKSSILDATIFLADKNDFAAMNKAWDAWVVAGHAPVRCTVQAGLMNP
                     KYKVEIKIVAAV"
     gene            2764..3123
                     /locus_tag="PN977_003819"
     CDS             2764..3123
                     /locus_tag="PN977_003819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015912513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1889 family protein"
                     /protein_id="HDI6043818.1"
                     /translation="MQIKVIYSLIDNMVNFKDKNMPAVIDKALDFIGAMDVSAPTPSS
                     MNESTAKGIFKYLKELGVPASAADITARADQEGWNPGFTEKMVGWAKKMESGERIVIK
                     NPEYFSTYMQEELKALV"
     gene            complement(3243..3422)
                     /locus_tag="PN977_003820"
     CDS             complement(3243..3422)
                     /locus_tag="PN977_003820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaH family protein"
                     /protein_id="HDI6043819.1"
                     /translation="MFAGLPSLTHEQQQKAVERIQELMAQGMSSGQAIALMAEELRAN
                     HSGERIVARFEDEDE"
     gene            3496..4857
                     /gene="pabB"
                     /locus_tag="PN977_003821"
     CDS             3496..4857
                     /gene="pabB"
                     /locus_tag="PN977_003821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 1"
                     /protein_id="HDI6043820.1"
                     /translation="MKTLSPAVITLPWRQDAAEFYFSRLSHLPWAMLLHSGYADHPYS
                     RFDIVVAEPICTLTTFGKETVISESDKRTTTTDDPLQVLQQVLDRADIHPTHNEDLPF
                     QGGALGLFGYDLGRRFESLPEIAQQDIVLPDMAVGIYDWALVVDHQRQTVSLLSHNDV
                     NARRAWLESQQFSPQEDFTLTSDWQSNMTRELYGEKFRQVQEYLHSGDCYQVNLAQRF
                     HATYSGDEWQAFLQLNQANRAPFSAFLRLEQGTILSLSPERFILCDNSEIQTRPIKGT
                     LPRLPDPQEDSKQAEKLANSAKDRAENLMIVDLMRNDIGRVAVAGSVKVPELFVVEPF
                     PAVHHLVSTITAQLPEQLHASDLLRAAFPGGSITGAPKVRAMEIIDELEPQRRNAWCG
                     SIGYLSFCGNMDTSITIRTLTAINGQIYCSAGGGIVADSQEEAEYQETFDKVNKILRQ
                     LEK"
     gene            4861..5439
                     /locus_tag="PN977_003822"
     CDS             4861..5439
                     /locus_tag="PN977_003822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HDI6043821.1"
                     /translation="MEYRSLTLDDFLSRFQLLRPQINRETLNHRQAAVLIPIVRRPQP
                     GLLLTQRSIHLRKHAGQVAFPGGAVDDTDASVIAAALREAEEEVAIPPSTVEVIGVLP
                     PVDSVTGYQVTPVVGIIPPDLPYRASEDEVSAVFEMPLAQALHLGRYHPLDIYRRGDS
                     HRVWLSWYEQYFVWGMTAGIIRELALQIGVKP"
     gene            5623..6987
                     /gene="sdaA"
                     /locus_tag="PN977_003823"
     CDS             5623..6987
                     /gene="sdaA"
                     /locus_tag="PN977_003823"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HDI6043822.1"
                     /translation="MISLFDMFKVGIGPSSSHTVGPMKAGKQFVDDLVEKGLLDSVTR
                     VAVDVYGSLSLTGKGHHTDIAIIMGLAGNEPATVDIDSIPGFIRDVEERERLLLAQGR
                     HEVDFPRDNGMRFHNGNLPLHENGMQIHAYNGDEVVYSKTYYSIGGGFIVDEEHFGQD
                     AANEVSVPYPFKSATELLAYCNETGYSLSGLAMQNELALHSKKEIDEYFAHVWQTMQA
                     CIDRGMNTEGVLPGPLRVPRRASALRRMLVSSDKLSNDPMNVIDWVNMFALAVNEENA
                     AGGRVVTAPTNGACGIVPAVLAYYDHFIESVSPDIYTRYFMAAGAIGALYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGGSPEQVCVAAEIGMEHNLGLTCDPVAGQVQVPC
                     IERNAIASVKAINAARMALRRTSAPRVSLDKVIETMYETGKDMNAKYRETSRGGLAIK
                     VQCD"
     gene            7118..8716
                     /locus_tag="PN977_003824"
     CDS             7118..8716
                     /locus_tag="PN977_003824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416329.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HDI6043823.1"
                     /translation="MQKAQRIIKTYRRNRMIVCTICALVTLASTLSVRFISQRNLNQQ
                     RVVQFANHAVEELDKVLLPLQAGSEVLLPLIGLPCSVAHLPLRKQAAKLQTVRSIGLV
                     QDGTLYCSSIFGYRNVPVVDILAELPAPQPLLRLTIDRALIKGSPVLIQWTPAAGSSK
                     AGVMEMINIDLLAAMLLEPQLPQISSASLTVDDRHLLYGNGLVDSLPQPENNENYQVS
                     SQRFPFTINVNGPGATALAWHYLPTQLPLAVLLSLLVGYIAWLATAYRMSFSREINLG
                     LAQHEFELFCQPLLNARSQQCIGVEILLRWNNPRQGWISPDVFIPIAEEHHLIVPLTR
                     YVMAETIRQRHVFPMSSQFHVGINVAPSHFRRGVLIKDLNQYWFSAHPIQQLILEITE
                     RDALLDVDYRIARELHRKNVKLAIDDFGTGNSSFSWLETLRPDVLKIDKSFTAAIGSD
                     AVNSTVTDIIIALGQRLNIELVAEGVETQEQAKYLRRHGVHILQGYLYAQPMPLRDFP
                     KWLAGSQPPPARHNGHITPVMPLR"
     gene            complement(8720..10276)
                     /gene="yoaE"
                     /locus_tag="PN977_003825"
     CDS             complement(8720..10276)
                     /gene="yoaE"
                     /locus_tag="PN977_003825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family cation transport protein YoaE"
                     /protein_id="HDI6043824.1"
                     /translation="MEFLMDPSIWAGLLTLVVLEIVLGIDNLVFIAILADKLPPKQRD
                     KARLLGLSLALIMRLGLLSLISWMVTLTKPLFTVMDFSFSGRDLIMLFGGIFLLFKAT
                     TELHERLENRDHDSGHGKGYASFWVVVTQIVILDAVFSLDAVITAVGMVNHLPVMMAA
                     VVIAMAVMLLASKPLTRFVNQHPTVVVLCLSFLLMIGLSLVAEGFGFHIPKGYLYAAI
                     GFSIIIEVFNQIARRNFIRHQSTLPLRARTADAILRLMGGKRQANVQHDADNPMPMPI
                     PEGAFAEEERYMINGVLTLASRSLRGIMTPRGEISWVDANLGVDEIREQLLSSPHSLF
                     PVCRGELDEIIGIVRAKELLVALEEGVDVAAIASASPAIIVPETLDPINLLGVLRRAR
                     GSFVIVTNEFGVVQGLVTPLDVLEAIAGEFPDADETPEIITDGDGWLVKGGTDLHALQ
                     QALDVEHLADDDDIATVAGLVISANGHIPRVGDVIDVGPLHITIIEANDYRVDLVRIV
                     KEQPAHDEDE"
     gene            10739..11710
                     /gene="manX"
                     /locus_tag="PN977_003826"
     CDS             10739..11710
                     /gene="manX"
                     /locus_tag="PN977_003826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HDI6043825.1"
                     /translation="MTIAIVIGTHGWAAEQLLKTAEMLLGEQENVGWIDFVPGENAET
                     LIEKYNAQLAKLDTTKGVLFLVDTWGGSPFNAASRIVVDKEHYEVIAGVNIPMLVETL
                     MARDDDPSFDELVALAVETGREGVKALKAKQVEKAAPAPAAAAPKAAPTPAKPMGPND
                     YMVIGLARIDDRLIHGQVATRWTKETNVSRIIVVSDEVAADTVRKTLLTQVAPPGVTA
                     HVVDVAKMIRVYNNPKYAGERVMLLFTNPTDVERLVEGGVKITSVNVGGMAFRQGKTQ
                     VNNAVSVDEKDIEAFKKLNARGIELEVRKVSTDPKLKMMDLISKIDK"
     gene            11773..12573
                     /gene="manY"
                     /locus_tag="PN977_003827"
     CDS             11773..12573
                     /gene="manY"
                     /locus_tag="PN977_003827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIC"
                     /protein_id="HDI6043826.1"
                     /translation="MEITTLQIVLVFIVACIAGMGSILDEFQFHRPLIACTLVGIVLG
                     DMKTGIIIGGTLEMIALGWMNIGAAVAPDAALASIISTILVIAGHQSIGAGIALAIPL
                     AAAGQVLTIIVRTITVAFQHAADKAADNGNLTAISWIHVSSLFLQAMRVAIPAVIVAL
                     SVGTSEVQNMLNAIPEVVTNGLNIAGGMIVVVGYAMVINMMRAGYLMPFFYLGFVTAA
                     FTNFNLVALGVIGTVMAVLYIQLSPKYNRVAGAPAQAAGNNDLDNELD"
     gene            12586..13437
                     /gene="manZ"
                     /locus_tag="PN977_003828"
     CDS             12586..13437
                     /gene="manZ"
                     /locus_tag="PN977_003828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020804907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IID"
                     /protein_id="HDI6043827.1"
                     /translation="MVDTTQTTTEKKLTQSDIRGVFLRSNLFQGSWNFERMQALGFCF
                     SMVPAIRRLYPENNEARKQAIRRHLEFFNTQPFVAAPILGVTLALEEQRANGAEIDDG
                     AINGIKVGLMGPLAGVGDPIFWGTVRPVFAALGAGIAMSGSLLGPLLFFILFNLVRLA
                     TRYYGVAYGYSKGIDIVKDMGGGFLQKLTEGASILGLFVMGALVNKWTHVNIPLVVSR
                     ITDQTGKEHVTTVQTILDQLMPGLVPLLLTFACMWLLRKKVNPLWIIVGFFVIGIAGY
                     ACGLLGL"
     gene            13492..13950
                     /locus_tag="PN977_003829"
     CDS             13492..13950
                     /locus_tag="PN977_003829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF986 domain-containing protein"
                     /protein_id="HDI6043828.1"
                     /translation="MTITDLVLILFIAALLAFAIYDQFIMPRRNGPTLLAIPLLRRGR
                     IDSVIFVGLIVILIYNNVTNHGALITTWLLSALALMGFYIFWIRVPKIIFKQKGFFFA
                     NVWIEYSRIKAMNLSEDGVLVMQLEQRRLLIRVRNIDDLEKIYKLLVSTQ"
     gene            14379..>14875
                     /gene="mntP"
                     /locus_tag="PN977_003830"
     CDS             14379..>14875
                     /gene="mntP"
                     /locus_tag="PN977_003830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP"
                     /protein_id="HDI6043829.1"
                     /translation="MNITATVLLAFGMSMDAFAASIGKGATLHKPKFSEALRTGLIFG
                     AVETLTPLIGWGMGMLASRFVLEWNHWIAFVLLIFLGGRMIIEGFRGADDEDEEPRRR
                     HGFWLLVTTAIATSLDAMAVGVGLAFLQVNIIATALAIGCATLIMSTLGMMVGRFIGS
                     IIGKKA"
BASE COUNT         3601 a         3745 c         3783 g         3746 t
ORIGIN      
        1 atactccgta acaaattacc tggaggatgg tatcgcaagg aagcgaaccg cacgggcaat
       61 gtcacgggtg cgcggcgcgg gcggcagact ggcgagaaac gtcgcgccgt aagggcgcat
      121 caccagccga ttgtcacaaa tcaccaaaac cccgcgatcg tcggcgtcgc gaatcagtcg
      181 ccctactccc tgcttgagag taatcaccgc atccggtagt tgtacttcat cgaacgggtc
      241 accaccacgc aaacggcaat cttccatgcg cgcttttaat agtggatcat ccggtgaggt
      301 aaacggcaac ttgtcgataa tcaccaatga caatgtatcg ccacgcacat ccaccccttc
      361 ccagaagctg ctggttgcca caagaagcgc gttaccggcg ctgacaaatt gctgcaacag
      421 ttgccctttg ctggtttccc cctgcaacaa aacgggaagc gtcatggtag cgcggaactg
      481 ctcagccaga tcgcgcatca tggcgtgcga ggtacaaagc ataaaacaac gaccgttgtt
      541 agcttcgatg atcggtcgca acattgccgc cagttgccgt gcggagcccg gttgattggt
      601 ttgcggcagg ttgcgcggca cacagagtaa cgcctggcgg ctgtaatcaa acgggctggg
      661 taacagcaac gactccgcct gttcgatgcc aagccgcgag gtgaaatgat gcagatcgtc
      721 gttcaccgac agcgttgctg aggtaaagat ccagctaccg ggtttttgcg ccattaactc
      781 tttgaattta tccgccacgc tgagcggcgt gagtgccaga gtaaaatggc gcgaagtgca
      841 ttcgtaccag tagctgtagc ccggctgatt gatctctttt agccgtttca gccgcgtgcg
      901 atacaacgtg gcgcgctcaa atgccgcatc cagcaaagcg gaacgcccca gcgacagttt
      961 cgccacgtca taacaaagtt ccagggtgtc atcgagcagt aaaaacgccc gttgaatttg
     1021 cggattagct aacagttcgc gcaggttgcc acgataacca ggctcaccga gttgcagacg
     1081 aaaatcctgc gcgctctggg caaggcggtc ggcgcacttt tgtaactgct gggtgtcttt
     1141 taattcggtg cggtaggcga tggtgatgtc ttttgccagg tcgagcagtt gtcgactgga
     1201 gagtgactga ccaaaatact ggctggcaat gtcgggcagt tggtgggctt cgtcgaagat
     1261 catgacgtca gcttccggga tcagttcgcc aaatccactc tctttcacca ccatatccgc
     1321 cagaaagaga tgatggttta ccaccaccac atcggcgtcc atcgcttttt tgcgtgcttt
     1381 gaccacaaag caatctttat acatcgggca gtcgctgcca aggcagttat cgttggtgct
     1441 ggtgaccagc ggccacgcct gtgaatcttc cgccacgctg acgcaggtgc tgatatcacc
     1501 atcgactgtt tgattagacc aggagcgcag caggatcaca tcgcttaaga tttgtaccgg
     1561 cagatcgccc cccgccagcg cctgctgttc gagacgttcg aggcagaggt agtttgagcg
     1621 ccctttcagc aacgccaggt tgcccgtgta tttcaatgcc tttgagaccg ttggcaaatc
     1681 gcggctgtag agctgatcct gcaacgcttt tgaaccggtc gagataatga ctttcttttt
     1741 cgcccgcagc gcaggggcca ggtaagcgta ggttttgccc gtaccggttc cggcttccac
     1801 caccagcggc tggccttttt ctatcgcctg ggtgacggct accgccatct gtcgctgtgg
     1861 ttctcgcggc ttaaagcctg gtatcgcttt cgccagctga ccgtctggtg caaaatcgtc
     1921 cgtcacacta ccccctgttg atttgaacag ggattatgtc aggatgaggg cgctttcgcc
     1981 agttgaagtg gtgacggcga cctcacattg tggcagtctt tgcagcacga aaatggaagg
     2041 ataatgagga caaaatgatg actatcgttc gtatcgatgc tgaagcccgc tggtctgatg
     2101 tggtgatcca caacaacacg ctatactaca ctggtgtacc ggaaaacctc gacgccgatg
     2161 cttttgagca aaccgccaac acgctggcgc agattgacgc cgtgctggaa aaacagggca
     2221 gcaataaatc gagcattctg gatgccacca ttttcctggc tgataaaaac gacttcgcgg
     2281 cgatgaataa agcgtgggat gcctgggttg tcgctggtca tgcgccggtg cgctgcacgg
     2341 tacaagcggg tttgatgaac ccgaagtata aagttgaaat taagattgtg gctgcagtgt
     2401 aagctttatc gaagcataat aagtcagacg ataatttatc gataatactg gtcggttttg
     2461 cataaatcga ccagagatta agttattact gttgatgctc ctgctaattc attatttatt
     2521 tttataattt caatttcatc tataaaactg gctatataaa taaacataac catgccgttg
     2581 tgttcgccct ctgattagca actctggttt tctttcttag ctgaacaatc tggttatagc
     2641 gtgagaactg accgttaatt ttctccctct tactggataa cacaccatga gctgctacct
     2701 ttaaaacacc tgaatgtcaa caggttaact cgtgccatat cgttataatc cctttgatat
     2761 tagatgcaaa ttaaggtcat atatagcctt atcgataaca tggttaattt taaggataag
     2821 aatatgcctg ctgtaataga taaagccctg gatttcattg gtgccatgga tgtatcagca
     2881 ccaacaccaa gttcgatgaa tgaaagcacg gcgaagggaa tctttaaata tttgaaagaa
     2941 ctgggagtac ccgcgagtgc cgctgatatt acggcgcgag ctgaccagga aggctggaac
     3001 ccggggttca cggaaaaaat ggttggatgg gcaaaaaaaa tggagtctgg tgaacgcatt
     3061 gtgattaaaa atcctgaata cttttcaaca tatatgcagg aagaactaaa agcactggtc
     3121 taagttaaat ttacatcagc ataaatgggt caggacgctt ttaatcacat atgaaaagcg
     3181 tcctttttct cacaatcaaa taatcatcgg cttctataac gaaagcgata cgggttagtg
     3241 tgttactcgt cttcatcctc aaaacgcgcc acgattcgct cgccggaatg gttggcacgc
     3301 agctcttctg ccatcaacgc gattgcctgg ccgctgctca tcccttgagc catcagttcc
     3361 tggatccgct cgacagcttt ttgctgctgt tcatgggtga gtgaaggtaa acctgcaaac
     3421 attgttaact cctgctaaat tgttggcgct aattatttca tgctacccgg cacatagcca
     3481 gtagagtcag gactgatgaa gacgttatct cccgctgtga ttactttacc ctggcgtcag
     3541 gacgccgctg aattttattt ctcacgctta agccacctgc cgtgggcgat gcttttacac
     3601 tccggctatg ccgatcatcc gtatagccgc tttgatattg tggtcgccga gccaatttgc
     3661 actttaacta ctttcggtaa agaaaccgtt attagtgaaa gcgataaacg tacaacgacc
     3721 actgatgacc cgctacaggt gctccagcag gtgctggatc gcgcagacat tcacccaacg
     3781 cataacgaag atttgccatt tcagggcggc gcgctggggt tgtttggcta cgatctgggc
     3841 cgccgttttg agtcactgcc agaaattgcg cagcaagata tcgttctgcc ggatatggca
     3901 gtgggtatct acgactgggc gctggttgtt gaccaccagc gtcaaacagt ttctttgctg
     3961 agtcataatg atgtcaatgc tcgtcgggcc tggctggaaa gccagcaatt ctcgccgcag
     4021 gaagatttca cgctcacttc cgactggcaa tccaatatga cccgcgaact gtacggcgaa
     4081 aaatttcgcc aggtacagga atatctgcac agcggtgatt gctatcaggt gaatctcgcc
     4141 cagcgttttc atgcgaccta ttctggcgat gaatggcagg cattccttca gcttaatcag
     4201 gccaaccgcg cgccgtttag cgctttttta cgccttgaac aaggcacgat tttaagcctt
     4261 tcgccagagc ggtttatcct ttgtgataac agtgaaatcc agacccgccc gattaaaggc
     4321 acgctaccac gcctgcccga tcctcaggaa gatagcaaac aagcagagaa actggcgaac
     4381 tcagcgaaag atcgtgccga aaatctgatg attgtcgatt taatgcgtaa tgatatcggt
     4441 cgtgttgccg tagcaggttc ggtaaaagta ccagagctgt tcgtggtgga acccttccct
     4501 gccgtgcatc atctggtcag caccataacg gcgcaactac cagaacagtt acacgccagc
     4561 gatctgctgc gcgcggcttt tcctggtggc tcaataactg gggctccgaa agtacgggct
     4621 atggaaatta tcgacgaact ggaaccgcag cgacgcaatg cctggtgcgg cagcattggc
     4681 tatttgagct tttgcggcaa catggacacc agcattacta tccgcacgct gactgccatt
     4741 aacggacaaa tttactgctc tgcggggggt ggaattgtcg ccgatagcca ggaagaagcg
     4801 gaatatcagg aaacttttga taaagttaat aagatattac gccaactgga gaagtaagac
     4861 gtggaatacc gtagcctgac gcttgatgat tttttatcgc gctttcaact tttgcgcccg
     4921 caaattaacc gggaaaccct aaatcatcgt caggctgccg tgttaatccc catcgtccgt
     4981 cgaccgcaac cggggttgtt gctgactcag cgttcgattc atctgcgcaa acacgctgga
     5041 caagtggcat tccctggagg cgcagtcgat gacacggacg catccgttat cgccgctgcg
     5101 ctgcgcgaag ctgaagaaga ggtcgctata ccgccttcca ccgttgaagt tatcggcgtg
     5161 ctgccgcccg tcgatagcgt cactggctac caggtaaccc cagtggtcgg cattatcccg
     5221 cccgatctgc cgtatcgcgc cagtgaagat gaagtctcgg cggtgtttga aatgccgctc
     5281 gcccaggcat tacatctggg tcgttatcat cctttagata tctaccgccg tggcgattca
     5341 catcgggtat ggctgtcctg gtacgaacag tattttgtat ggggaatgac cgcaggcata
     5401 attcgtgaac tggcgctgca aattggtgtg aaaccctgac tatacttatc tttacatcta
     5461 caaaacacta cttgagacaa tcatcgcaat attagttaaa tcgcggtttt tgattagttt
     5521 aattcatgtg aatagttaag ccagtcgccg cgttccctct tacactatgc gctgttatta
     5581 gttcgttact ggaagtccag tcaccttgtc aggagtatta tcgtgattag tctattcgac
     5641 atgtttaagg tggggattgg tccctcatct tcccataccg tagggcctat gaaggcgggt
     5701 aaacagttcg tcgatgacct ggtcgaaaaa ggcttactgg atagcgttac tcgcgttgcc
     5761 gtggacgttt atggttcact gtcgctgacg ggtaaaggcc accacaccga tatcgccatt
     5821 attatgggtc ttgcaggtaa cgaacctgcc accgtggata tcgacagtat tcccggtttt
     5881 attcgcgacg tagaagagcg cgaacgtctg ctgctggcac agggacggca tgaagtggat
     5941 ttcccgcgcg acaacgggat gcgttttcat aacggtaacc tgccgctgca tgaaaacggt
     6001 atgcaaatcc acgcctataa cggcgatgaa gtcgtctaca gcaaaactta ttattccatc
     6061 ggcggcggtt ttatcgtcga tgaagaacac tttggtcagg atgccgccaa cgaagtgagc
     6121 gtgccgtatc cgttcaaatc tgccaccgaa ctgctcgcgt actgcaatga aaccggctat
     6181 tcgctgtctg gtctcgctat gcagaatgaa ctggcgctgc acagcaagaa agagatcgac
     6241 gagtatttcg cgcatgtctg gcaaaccatg caggcatgta tcgatcgcgg gatgaacacc
     6301 gaaggcgtac tgccaggccc gctgcgcgtg ccgcgtcgtg cgtctgccct gcgccggatg
     6361 ctggtttcca gcgataaact gtctaacgat ccgatgaatg tcattgactg ggtaaacatg
     6421 tttgcgctgg cagttaacga agagaacgcc gccggtggtc gcgtggtaac tgcgccaacc
     6481 aacggtgctt gtggcattgt tccggcagtg ctggcttact atgaccactt tattgagtcc
     6541 gtcagcccgg acatctatac ccgctacttt atggcagcgg gtgcgattgg tgcactgtat
     6601 aaaatgaacg cctctatttc cggtgcggaa gtcggttgcc agggcgaagt gggtgttgcc
     6661 tgttcaatgg ctgctgcggg ccttgccgag ctgttgggcg gtagcccgga acaggtttgt
     6721 gtggcggcgg aaattggcat ggagcacaac ctcggtctga cctgcgaccc ggttgcaggg
     6781 caggttcagg tgccgtgcat tgagcgtaat gctattgcct ctgtgaaggc gattaacgcc
     6841 gcacggatgg ctctgcgccg caccagcgca ccgcgcgtct cgctggataa ggtcatcgaa
     6901 acgatgtacg aaaccggtaa ggacatgaac gccaaatacc gcgaaacctc acgcggtggt
     6961 ctggcaatca aagtccagtg tgactaatac ttcttactcg cccatctgca acggatgggc
     7021 gaatttatcc ccgctttctc gtctgctgta atattcccca ctacacttcc actgttgcgt
     7081 caggcgtttg tcgccatacg cttacagggt ggcccgcatg caaaaagcac aacggatcat
     7141 taaaacctat cgccgtaatc gaatgattgt ttgtacgatt tgcgccctcg ttacgctcgc
     7201 ttcgaccctg agcgtgcgat ttatttcaca gcgtaactta aatcaacaac gggtagtaca
     7261 attcgccaat cacgctgtag aggaattaga taaagtactg cttcccctac aggcaggtag
     7321 cgaagtcttg cttccgctga ttggtctgcc ctgctctgtc gcccatttgc cattacgtaa
     7381 acaggcggca aaactccaaa ctgtgcgatc cattggcctg gtgcaagacg gcacacttta
     7441 ttgctccagc atttttggtt atcgcaatgt gcccgtcgtg gacattctgg ctgaacttcc
     7501 tgcaccgcaa ccacttttac gcctgacgat cgaccgtgcc ctgattaaag gcagtccggt
     7561 tttgattcag tggacgccag cagcgggcag tagcaaagct ggggtcatgg agatgattaa
     7621 catcgactta ctggcggcaa tgctgcttga gccacaactg ccgcaaatca gtagcgccag
     7681 cctgacggtg gacgatcggc atttgctcta tggtaatggg ctggtagatt cccttccgca
     7741 acctgaaaac aatgaaaact accaggtttc ttcgcaacgc tttcctttta ccattaacgt
     7801 taatggtccg ggggctacgg cgctggcatg gcactatctt ccaacacaat taccgctggc
     7861 ggtgctgcta agtttactgg tgggctacat cgcctggctg gcgaccgctt accggatgag
     7921 cttttcccgc gaaatcaatc tgggcctggc gcaacatgag ttcgaattgt tctgtcagcc
     7981 tttgcttaat gcgcgcagcc agcaatgtat tggtgtagag attttgcttc gctggaacaa
     8041 tccgcgtcag ggctggattt caccggatgt gtttattcct atcgcggaag aacatcattt
     8101 aattgtgcca ctgacccgct atgtgatggc agaaaccatt cgtcagcgcc atgttttccc
     8161 gatgagtagt cagtttcatg ttggcattaa cgtcgcaccc agccattttc gccgtggtgt
     8221 gctgataaaa gatctcaatc agtactggtt tagcgctcac ccgattcagc aactgatcct
     8281 cgaaatcacc gaacgcgatg ccttactgga tgttgattat cggattgccc gcgagctaca
     8341 tcgtaaaaac gtcaaactgg cgattgatga cttcggcacc ggcaacagtt cattttcctg
     8401 gcttgaaaca ttacgtcctg acgtgctgaa aattgataag tcatttaccg cagctatagg
     8461 ttctgacgcg gttaactcga cggtgaccga tatcatcatc gctctggggc aaagactgaa
     8521 tattgaactg gtggcggagg gtgtggaaac acaagaacag gcgaagtatt tgcgccgtca
     8581 tggggtgcat attttgcaag ggtatttgta cgcacagccg atgccgctac gtgattttcc
     8641 caaatggctg gcgggcagcc aaccgccgcc cgcccggcat aatggacata tcacgcccgt
     8701 tatgccgtta cgttaacact tactcatctt catcgtgcgc cggttgctct ttaacaatgc
     8761 gaaccagatc aacacgataa tcattggctt caatgatggt gatatgcagt ggccctacat
     8821 caatcacatc gcccacacgg ggaatgtgac catttgccga gatcacgagg cccgcgaccg
     8881 tcgcgatatc atcgtcatcg gcaaggtgct caacatcaag cgcctgctgc aaggcatgca
     8941 aatctgtacc gccttttacc agccagccgt caccgtcggt aatgatttcc ggcgtttcgt
     9001 cagcgtccgg gaattcaccc gcaatggctt ccagcacatc cagcggcgtg accagacctt
     9061 gtaccacacc aaactcgttg gtcacgataa caaagctccc gcgagcacga cgcagtacgc
     9121 ccagcaggtt gatcggatcg agggtttccg ggacgataat cgccggagac gccgaagcaa
     9181 tcgccgccac atcaacgccc tcttccagcg ccaccagcag ttctttagca cgtacgatac
     9241 cgatgatttc atccagttca ccgcgacata ccgggaacag actgtgcggt gaagagagca
     9301 gttgctcgcg gatttcatcg accccgagat tagcgtcaac ccagcttatt tcaccgcgcg
     9361 gcgtcatgat cccgcgcaga gaacgcgacg ccagcgtcag tacgccgtta atcatgtaac
     9421 gttcttcttc ggcaaatgca ccttccggga tcggcatcgg catcgggtta tcggcatcgt
     9481 gctggacatt ggcctgacgt ttcccgccca tcaaacgcag gatggcatcg gcagtacgcg
     9541 ctcgcagcgg caaagtcgac tggtgacgaa taaagttgcg acgcgcaatt tggttaaaca
     9601 cttcgatgat gatcgagaag ccaatcgcgg catacaggta acctttcgga atgtggaaac
     9661 cgaaaccttc tgccaccaga ctcagaccta tcattaacag gaagctcaga cagagcacca
     9721 ccaccgttgg atgctggtta acaaatcgcg tcagcggttt cgatgccagc agcataaccg
     9781 ccatcgcaat cactaccgcc gccatcatca ctggcagatg gttaaccatc cctactgccg
     9841 taattaccgc atccaacgag aagacggcgt caaggatgac gatctgtgtg acgaccaccc
     9901 agaaactggc gtagccttta ccgtggccgg aatcatgatc gcggttttcc agccgttcat
     9961 gcagttcggt tgttgctttg aacagcaaga atatcccccc gaacaacata atcaggtcgc
    10021 gtccggagaa ggagaaatcc atgacggtaa atagcggttt ggtcagcgtg accatccatg
    10081 aaatcagcga cagcagcccc agacgcataa tcagcgccag tgataatccc agcaaacgcg
    10141 ctttatcgcg ttgttttggc ggcagtttgt cagcaagaat ggcgatgaag accaggttat
    10201 cgatacccag cacaatttcg agaacaacaa gcgtgagtag ccccgcccaa atcgaggggt
    10261 ccattaagaa ttccatgaca agctcctgct taaggaatag ctattcgacg ccagaaataa
    10321 tgcaggcgta acgacaaaat gcaaacgaaa ggtgcggcat aaagtgccag aaaggcaggc
    10381 gttaaaaggc ctgatgctga aatgacgtcg gtgacgatcc atactgcggg ctactgccct
    10441 atactccatg gttgttaaac aggagttaaa catatcagag acacatctga tttggcaaag
    10501 atttaccttc ctttgcaaac gaatgtgaca aggatatttt acctttcgaa atttctgcta
    10561 atcgaaagtt aaattacgga tcttcatcac ataaaataat tttttcgata tctaaaataa
    10621 atcgcgaaac gcaggggttt ttggttgtag cccttatctg aatcgattcg attgtggacg
    10681 acgattcaaa aatacatctg gcacgttgag gtgttaacga taataaagga ggtagcaagt
    10741 gaccattgct attgttatag gcacacatgg ttgggctgca gagcagttgc ttaaaaccgc
    10801 agaaatgctg ttaggcgagc aggaaaacgt cggctggatc gatttcgttc caggtgaaaa
    10861 tgccgaaacg ctgattgaaa agtacaacgc tcagttggca aaactcgaca ccactaaagg
    10921 cgtgctgttt ctcgttgata catggggagg cagcccattc aatgctgcca gccgcattgt
    10981 cgtcgacaaa gagcattatg aagtcattgc aggcgttaac attccaatgc tcgtggaaac
    11041 gttaatggcc cgtgatgatg acccaagctt tgatgaactg gttgcgctgg cagtagaaac
    11101 aggccgtgaa ggcgtgaaag ctctgaaagc caaacaggtt gaaaaagccg cgccagcacc
    11161 cgctgccgca gcaccaaaag cggctccaac tccggcaaaa ccaatgggac caaacgacta
    11221 catggttatt ggccttgcgc gtatcgacga ccgtctgatt cacggtcagg tcgccacccg
    11281 ctggaccaaa gaaaccaatg tctcccgtat tattgttgtt agtgatgaag tggctgcgga
    11341 taccgttcgt aagacactgc tcacccaggt tgcacctccg ggcgtaacag cacacgtagt
    11401 tgatgttgcc aaaatgattc gcgtctacaa caacccgaaa tatgctggcg aacgcgtaat
    11461 gctgttattt accaacccaa cagatgtaga gcgtctcgtt gaaggcggcg tgaaaatcac
    11521 ctctgttaac gtcggtggta tggcattccg tcagggtaaa acccaggtga ataacgcggt
    11581 ttcggttgat gaaaaagata tcgaggcgtt caagaaactg aatgcgcgcg gtattgagct
    11641 ggaagtccgt aaggtttcca ccgatccgaa actgaaaatg atggatctga tcagcaaaat
    11701 cgataagtaa cgtattgtgt tgattatcac tcagttttca cacttaagtc ttacgtaaac
    11761 aggagaagta caatggagat taccactctt caaattgtgc tggtatttat cgtagcctgt
    11821 atcgcaggta tgggatcaat cctcgatgaa tttcagtttc accgtccgct aatcgcgtgt
    11881 accctggtgg gtatcgttct tggggatatg aaaaccggta ttattatcgg tggtacgctg
    11941 gaaatgatcg cgctgggctg gatgaacatc ggtgctgcag ttgcgcctga cgccgctctg
    12001 gcttccatca tttctactat tctggttatc gcaggtcatc agagcattgg tgcaggtatc
    12061 gcactggcaa tccctctggc cgctgcgggc caggtactga ccatcatcgt tcgtactatc
    12121 accgttgctt tccagcacgc tgcggataag gctgctgata acggcaacct gacagcgatt
    12181 tcctggatcc acgtttcttc tctgttcctg caagcaatgc gtgtggctat tccggccgtc
    12241 atcgttgcgc tgtctgttgg taccagcgaa gtccagaaca tgctgaatgc gattccggaa
    12301 gtggtgacca atggtctgaa tatcgcaggt ggcatgatcg tagtggttgg ttatgcgatg
    12361 gttatcaaca tgatgcgtgc tggctacctg atgccgttct tctacctcgg cttcgtaacc
    12421 gcagcattca ccaactttaa cctggttgct ctgggtgtga ttggtactgt tatggcagtg
    12481 ctctacatcc aacttagccc gaaatacaac cgcgtagccg gtgcgcctgc tcaggcagct
    12541 ggtaacaacg atctcgataa cgaactggac taacaggtga gcgaaatggt tgatacaact
    12601 caaactacca ccgagaaaaa actcactcaa agtgatattc gtggcgtctt cctgcgttct
    12661 aacctcttcc agggttcatg gaacttcgaa cgtatgcagg cactgggttt ctgcttctct
    12721 atggtaccgg caattcgtcg actctaccct gagaacaacg aagctcgtaa acaagctatt
    12781 cgccgtcacc tggagttctt caacacccag ccgttcgtgg ctgcgccgat tctcggcgta
    12841 accctggcgc tggaagaaca gcgtgctaat ggcgcagaga tcgatgacgg tgctatcaac
    12901 ggtatcaaag tcggtttgat ggggccgctg gctggtgtag gcgacccgat cttctgggga
    12961 accgtacgtc cggtatttgc agcgctgggt gccggtatcg caatgagcgg cagcctgtta
    13021 ggtccgctgc tgttcttcat cttgtttaac ctggtgcgcc tggcaacccg ttactacggc
    13081 gtagcgtatg gttactccaa aggtatcgat atcgttaaag atatgggtgg tggcttcctg
    13141 caaaaactga cggaaggggc gtctatcctc ggcctgtttg tcatgggggc attagttaac
    13201 aagtggacgc atgtcaacat cccgctggtt gtctctcgca ttactgacca gacgggcaaa
    13261 gaacacgtta ccactgtcca gactattctg gaccagttaa tgccaggcct ggtaccactg
    13321 ctgctgacct ttgcttgtat gtggctactg cgcaaaaaag ttaacccgct gtggatcatc
    13381 gttggcttct tcgttatcgg tatcgctggt tacgcttgcg gcctgctggg actgtaagac
    13441 tgttgtacac taccggggcc ttttggcccc gtttttttat ctggaggatt aatgacaatc
    13501 acggacctgg tactgattct tttcatcgcc gcactcctgg ccttcgcgat ctacgatcag
    13561 ttcatcatgc cccgccgtaa cggccccact ctgctggcaa ttcctttgct ccggcgtggt
    13621 cgcatcgata gcgttatatt cgtcggattg attgtcattc ttatctataa caacgtcacg
    13681 aatcatggcg cgctaataac aacatggtta ttaagtgcac tggctctgat gggtttttat
    13741 atattctgga tccgcgttcc gaagatcatc tttaaacaaa aaggtttttt cttcgccaat
    13801 gtctggattg aatatagccg aatcaaagcg atgaacttgt cggaagatgg cgtgttggtg
    13861 atgcaattag aacaacgtcg gctgttaatc cgcgttcgaa atatcgacga tctggaaaaa
    13921 atttataagc ttctcgtttc aactcaatag gttatgaatt tagccaaagc tatgtttagt
    13981 gtatttttaa taatcagaca tagcttaggc tatattacct cttcccttat ttgttattta
    14041 ttttaacgtt tcattgatat ataaatctaa atgaaaatcg ttatcaataa agtgatgaaa
    14101 taatatattc caacagttgt tttatattct caaaatatgt taaggttgcg ccctcatttg
    14161 gggagtagcc gatttccaga ttccggaaat gtacgtgtca acatactcgt tgcaaaacgt
    14221 ggcacgtacg gactgaacac tttcagtcag gcgagaccat atgcacatca atcgctatgc
    14281 ctgcatgaac gcgaaaacca tcgcggtttg ttgttcatgt atgcatgttt gctgggggcg
    14341 atgatgtgtt ttatggatac cccggtcagg acgttgtcat gaatatcact gctactgttc
    14401 ttcttgcgtt tggtatgtcg atggatgcat ttgctgcatc aatcggtaaa ggtgccaccc
    14461 tccataaacc gaaattttct gaagcgttgc gaaccggcct tatttttggt gccgtcgaaa
    14521 ccctgacgcc gctgatcggc tggggaatgg gcatgttagc cagccggttt gtccttgaat
    14581 ggaaccactg gattgcgttt gtgctgctga tattcctcgg cgggcgaatg attattgagg
    14641 gttttcgtgg cgcagatgat gaagatgaag aaccgcgccg tcgacacggt ttctggctac
    14701 tggtaaccac cgcgattgcc accagcctgg atgccatggc tgtgggtgtt ggtcttgctt
    14761 tcctgcaggt taacattatc gcgaccgcat tggccattgg ttgtgcaacc ttgattatgt
    14821 cgacattagg gatgatggtt ggtcgcttta tcggctcaat tattgggaaa aaagc
//
LOCUS       DAMZFT010000120        14796 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.120, whole genome shotgun sequence.
ACCESSION   DAMZFT010000120 DAMZFT010000000
VERSION     DAMZFT010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14796)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14796)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14796
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.120"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..617
                     /locus_tag="PN977_003831"
     CDS             <1..617
                     /locus_tag="PN977_003831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000783678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HDI6043830.1"
                     /translation="PKYRDAYSTHIPAVILAVNNNPMRFTDRSGGVSRRRVIIHFPEQ
                     IAPQERDPQLKDKITRELAVIVRHLMQKFSDPMLARSLLQSQQNSDEALNIKRDADPT
                     FDFIGYLETLPQTSGMYMGNASIIPRNYRKYLYHAYLAYMEANGYRNVLSLKMFGLGL
                     PVMLKEYGLNYEKRHTKQGIQTNLTLKEESYGDWLPKCDDPATT"
     gene            1385..2212
                     /locus_tag="PN977_003832"
                     /pseudo
     CDS             1385..2212
                     /locus_tag="PN977_003832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001362050.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase arm-type DNA-binding domain-containing
                     protein"
     gene            2287..2486
                     /locus_tag="PN977_003833"
                     /pseudo
     CDS             2287..2486
                     /locus_tag="PN977_003833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611456.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(2684..3577)
                     /locus_tag="PN977_003834"
     CDS             complement(2684..3577)
                     /locus_tag="PN977_003834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000291809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HDI6043831.1"
                     /translation="MYYSKFLEKHKSPLSHAQWLRLRYIERQLLWERKVTSRMIAEEF
                     GVSAQQARTDVRTYMEVAPGNAIEKGAGRKGYFPSTTFTPVLLGDNDLVWRTTVEFQP
                     PQAAHAQEIPLIHRAVHSRTLAIFLSAIEQKGQVKVTYASMENPEGISRTLSPTVIIT
                     VNGRQHVRAYDWGSSAFKDFVLARFLDLPELIVPAPAPPRKDTAWEVIIPVRFIANPT
                     LNKAQQEVIKRDYLLEPSEFNIRAPMIFYLCPENNLPKTDDEYAQATDASSHGPFIYP
                     VLAVHAQTGEPIHKYRHAGER"
     gene            3753..5219
                     /locus_tag="PN977_003835"
     CDS             3753..5219
                     /locus_tag="PN977_003835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6043832.1"
                     /translation="MSLGFLEQARNESMARRELSIQSHALLVVRTLDSIYSALQRNPF
                     DGVELDALAAIMYLFDAMLYYADRERQPGMTRKEAELCLVGHLNEAYPGQEEPDYAQV
                     ASKIFAAIKDPFRLRYYDFSQRTHSEEQIVRLVRYDQLSGSDDILWSLTDEGMLFYTL
                     RLDETPIERAAILAWRTLKTIRRGEIDKAVHQIQSTQRQLEQYLIGMRMKVRAAQSGD
                     LSASYAHDIRPVLNDSFDKTREVLEHIQNSMSAITEMLVEGSSKINPDKLNKLRRAQE
                     IFAEVCAFTQRYQDCLLDVNKDFERARLKLIIPRDNTLFRETLEKGALKPLLSHSPDT
                     VEKEIDDLTARLLAPRRQDDDRHILFDLEALLNLYLDRFEQALPHEIQDLTDQPVAIE
                     TNLRGIPESVIKKCSDWVSDRLKNWGHVSFRDVFDAYEGEELSEQEQQVCLLHIGSLA
                     ANSDPAIEVEIDGAIETTLWRANNIKLLTVAPLYPFEE"
     gene            5222..6118
                     /locus_tag="PN977_003836"
     CDS             5222..6118
                     /locus_tag="PN977_003836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000272178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6043833.1"
                     /translation="MYDNAHQQTKRYAEIATQAIMNSLGYKMGDELIQRALDLQGDAL
                     FTEIIRGVATGMKLRYLGIHETYGVLLAADGRGPFSPSLGWLNKRLLPETMAEQQGKE
                     RKEQRLFAGLTLIALLADLFPTPDTVRVEFGQSNAINAASVVARLNNVADQVIAQRKA
                     EGDDTVDGLLMAARFVQEERIPRIHDEKVGRVSSIRSQTEMATRWIQLLQEEGFLAED
                     SSLSGESDWYPTQKLKHYLRHTGIEETVRFVMSISPAVEERSPDLAESDLAALADTLE
                     FPSLNERPVTTGTTETQRLTEI"
     gene            6121..10557
                     /locus_tag="PN977_003837"
     CDS             6121..10557
                     /locus_tag="PN977_003837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020079752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6043834.1"
                     /translation="MWLIKRVYMDGAGHEEAFYKDLTLDYIGLSQAVSSPVVEGLRSP
                     VRNAKVVTSTYQQLVNGGGKSTYIALLLSIFEPSVSDFTQYLANHRQSEYHYRNYFYK
                     ELSVVLVEMVNESGQTLLLGHAHQRNGDDVDRTLFICDAPDSLMGAPFDEVPSYSRAE
                     RNSELRPYANSLRSFDSWLNLKAGSEGGMHWRKTTRQNEWRAFLLEQQINVDLIKTMV
                     TFNSEEGGLTRFIEYKTEHDFLGAFFACTMSKDAAERLRELVAHEVERQGELEGIRRN
                     ESFLKDVLANWLAFLDPAKQLESIQAQQQLLDDGFREAMRDLAAFVGDAQVEQTQLED
                     NMDSLQTHIGQAMSLREQLGKRRALLEYDLVQHRFNKQKAIVDALQDEADTNQRQIEI
                     THAAIDYKAYAKALNEYRQADEDLKVFQSETERPLQVIFERTAGEALGTLEWAIDRHR
                     QQEATLEEELGQMDRESHVLRQSYDKIQKTLGGLETERDGYKRDKFQGESQRNELCEA
                     GLLQKDETPVAAQYRLELTLRDAKQSEELANTILHQKEQALEAQLNVVREASIEKSEA
                     QQKLILAEQKHQSAVDSALVLAKNYQKLPTSTRHDLDIEDTSWHSELCGLQLDKVLNA
                     NAQTLNGMKERLLQARHELDELRVTGHELVAKQVNEALEAFYAAGIARDKLWTFPHYL
                     ASAYNDDAQKIAAVIDQNPGRYLSLVALDNVTFEQVHKISEILPWKKAPIAIYLLDEA
                     QDLRRETSELVQVVLSNPDKHGYSQAAYETRLQDLENTVASQEVSVKATESALKVLES
                     FRNDWSRHYEQYGKHWAGLLDQRELALLILENADAALKQAEHDEQTLRDEKRDVQVRL
                     NNAQQQKIQAEKAHDKVSLFVEKEWAKRETAIMKLEQLAAQIQRSKNTLYTQRERLER
                     LSELEKNKRSEKTLLIIQIGSWSNLCNEPFYSEITATEGMEGRSPEYAHEMAAQAHKK
                     LLEAANGDQVTVLKQQLKDAEQVQRYASDRLTAQPTYNTYPDEVKAAAINELTVINNS
                     IVRLKEQQKQFTQNLISSQVTLQKFSQSCEEASKALDEDFVWEEKPIEIEAIQTSLAE
                     IVNNLYAIEHQIKFLLEQKTLLLRKIENVKERLQNAKTAQAMIQPITPSSAAGLPGRI
                     LSITEYADDLAAQCQVYAGLNETLKQQNMRVSTVWGKFRKRIEEELAKDPDARASQEY
                     LRQLDSIATYHEVLGDLERVGTGISQVFEAVQDSLEQFQRSIDLTVTHLTAHLEGAIK
                     LLRRAMSVKIPEDCPVLPGRPIIKMTDRLNDVAADLRGFASSRLQQWIARGQIPRAPN
                     RDALTADLVQSVFGEGELEVRLLKTNASRPQWTPVTRLEGSGGQRLTSAFLLFVTVGK
                     VREYDTGISSAGFLLADNPLGKSNANDLMRIQTQMAKAYKIQLIYLTGISDENAQSMF
                     DNHLFLNKVQKLRRRDLVTVDKERHALWAASLAAKPQFDPVI"
     gene            10582..11625
                     /locus_tag="PN977_003838"
     CDS             10582..11625
                     /locus_tag="PN977_003838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020079751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6043835.1"
                     /translation="MIWLSRLESTANKHRLAAGKAARTVKANAVLDEVLRAHNQEALS
                     QEVPDLLSQAVTQLLERDWRAQPKHKASWQRYWRGMSLPSTFVYEGAIVPTKTTSEIA
                     WHPWLARRITGQLTQPIKARLQQLNTYLFRQTGGDKSLFPGLLGHRERSLLIFGDEKA
                     LDSLPPDGWKHVTLTLADMGAFRRAPPLPYESSRCHDLPAIIVENSDVYYRLCQLNRA
                     QTRWSLVIYGAGNKVSGQAECVSQLLEVEQVEQLLYFGDLDVAGLKIAHQLRCKLLND
                     YGISLHLDEWLYNKLILNGLMTAEGNANNERFDFELQCGWMPKFVLREMKTLLAIHQR
                     LPQEGVASLGLFI"
     gene            11752..12750
                     /locus_tag="PN977_003839"
     CDS             11752..12750
                     /locus_tag="PN977_003839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004019902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6043836.1"
                     /translation="MALYNIVNKELHALEKTTFTLEGLQERYDLQEAIKKNIDIIAPD
                     CLVISEEFSDWEDSRRRIDLLAIDKQANLVVIELKRDETGAHMELQALRYAAMISTMS
                     FAKACEYFQTYLKKQNCDADAKEKILEFVELDETELVDFGKDIRIVLASSDFSKELTT
                     TAIWLRDKGVDIRCVRLTPYRFNDDVLINAEQIIPVPELEEYQVKFREKRDEQLISSQ
                     KKEKDYTWYIYKDKELNKRKLALELLRDWIRQFNPASYNDLINGLSDDFKKRTVMLID
                     QIPEKQKSCYHINEDALITLPSGEIVAISNQWGIVNIELLIEFVRQNGFVVEKAEQ"
     gene            complement(12862..13734)
                     /locus_tag="PN977_003840"
     CDS             complement(12862..13734)
                     /locus_tag="PN977_003840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004186812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HDI6043837.1"
                     /translation="MASSKSLQQAIANIKIWHKGEQRAPHKPLLLLYVLAGYLNGHPR
                     LFDYGLEIYEPLHNLLERFGPQRSQYRPDMPFWRLQGDGFWQLHNAELCSTAGSSRQP
                     PVKELNEYHVAGGFDEQHYALVTGNKKLINTLAQQILEAHFTESIQEEIADELGFDLQ
                     QIRKQRDPLFRKNVLRAYNYQCAICGFNMRHDDTTVALEAAHIKWKQHGGPCEIPNGL
                     ALCAIHHKAFDKGSIGLDENMRVLVSDAVNGGGIVERLFWDFDGKTIALPQVRKNYPF
                     EGFVEWHRKEVFRG"
     gene            13871..14512
                     /locus_tag="PN977_003841"
     CDS             13871..14512
                     /locus_tag="PN977_003841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001586610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HDI6043838.1"
                     /translation="MNKLPDHLPRRYIIEAIHLWDAGETHQFQPARLYKIEYHGRRYP
                     SKAIAGIAATLMTGTQFTPADFTGGIKSKCVKLLIEQGFHIHQDKAAAAPIADVLFPD
                     ELPSQETYVEGAAIKVTVNRYERDKKARDKAVEHHGCQCNVCGVDLVKIYGDIAEGFI
                     HMHHLVPLSAIKEDYQLDPVNDLLPVCPNCHAMLHRRKPPFSPEQLKALMDAN"
     gene            complement(14520..14636)
                     /locus_tag="PN977_003842"
                     /pseudo
     CDS             complement(14520..14636)
                     /locus_tag="PN977_003842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001486325.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
     gene            complement(14631..14796)
                     /locus_tag="PN977_003843"
                     /pseudo
     CDS             complement(14631..14796)
                     /locus_tag="PN977_003843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418730.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminic acid mutarotase"
BASE COUNT         4085 a         3309 c         3686 g         3716 t
ORIGIN      
        1 acccgaaata ccgggatgca tactccacgc atatcccggc ggtgattctg gccgtgaaca
       61 ataacccgat gcgcttcacc gaccgcagcg gcggcgtgtc acgccggcgg gtgattattc
      121 acttcccgga acagatagcc ccgcaggagc gcgacccgca gcttaaggac aaaatcaccc
      181 gcgagctggc ggtcatcgtg cgtcacctga tgcagaaatt cagcgacccg atgctcgccc
      241 ggtcactgct tcagtcccag caaaactcag acgaggcact gaacatcaaa cgggatgccg
      301 acccgacgtt tgattttatc ggctatctgg aaaccctgcc gcagaccagc ggcatgtata
      361 tggggaacgc cagtatcatc ccgcgtaatt accgtaaata cctctatcac gcctatctgg
      421 cctacatgga ggcaaacggc taccggaatg tactcagtct gaaaatgttc gggctggggc
      481 tgccggtgat gctgaaggaa tacggactga attacgagaa gcgccatacc aaacagggga
      541 tacagaccaa cctgacgctg aaagaggaaa gctacggcga ctggctgcca aaatgtgacg
      601 accctgcaac aacctgacca tcatgactca tctgaccggc atctgccggt cttttttcat
      661 ccctgaatcc cccgaaggtg aacaatccag tgttcaccct tcaccgtata ttcacccgtt
      721 atcacactga aattaaaaga gaaaaacgaa aggcgaacag tgtgaacaat caaatcaaaa
      781 aaaacttttt ttcttcctgt gtgattttca gtgcggagga ttaatcaccg gtatgagtca
      841 caccggcaga atgccggagg tgaagaatcg aatgttcacc cttcacccat tattcaccac
      901 ctatcatact gaaataaaag gagaaaaaag aaaggtgaac agtgtgaaca gttctttcga
      961 aaaaaaattt ttatgtggat aaaagatgca ctggtttgga tccgggctac ggatccaaac
     1021 ttactgatta aaaattattt aactttccga tttattgatg ggctgaaagt agattggtca
     1081 gaaaaacagt gggggcacaa aagggggcat atttcattgt tgtgttttat tatttgtttt
     1141 ttatcattaa cttattttaa tgtttgagtc cggccttcgc accaaaagta tgtaaataga
     1201 cctcaactga ggtctttttt tatgcctgaa atccgcgcca cgcaaggctt ttccagcttt
     1261 tcttctcaac tgaagtcaac ctgactcaac ccacatcaac aggcttgtga gtatacttat
     1321 gagtatattt gccgattcga tattgcttgt atactcacga gataccaatg gaaggaagac
     1381 cattattgcc ctaacggata ctaaagttcg ttctgtgaag tcagaggaga aagagtattc
     1441 acttgttgac ggcgacggca tgtctttact tgtaaaacct ggcggttcaa agtattggtg
     1501 atttcgtttc cgttttggtg gtaaacaaca tttgatggcg ttcggcgttt acccggatgt
     1561 ttcgctggcg gatgcgcgga agaaaaggga agaggccaga aagctggtcg ctgctggtat
     1621 cgatcctcgt gagcataaac gtgctgtgaa agaagagcag gcgaaagaga ttattacttt
     1681 cgagaaggtt gccagagagt ggctcgtgac aaaccaaaaa tggtcggaag atcatgctaa
     1741 tcgtggaaaa aagagcctgg aggacaatat cttcccaaca attggtgctc gcaatattgc
     1801 tgaactgggc acccgcgatc tgttaattcc cattaaagcc gtggagaaat ctggacgttt
     1861 tgaagtggct tcccgccttc aacagcgcac cacggccatc atgcgttatg cagtgcaaag
     1921 cgggttaatt cattacaatc cggcgcagga gatggcgggg gcggttgctt ccagtaatcg
     1981 acagcatcgt ccggcgctgg agttaaagcg catccctgag ttacttgaga aaatagacag
     2041 ctataccggc aggccgctaa cccgctgggc gacagaactc actctgctta tctttattcg
     2101 atccagtgag ctgcgttttg ctcgctggtc agagagcgat tttgatacgt caatgtggac
     2161 catccctctt gatgggcatt gcagtaagct gagggttgga ctggtattgt aaatatcccg
     2221 cataatcgtg ccattaacat ttagagatcc tccggcataa tcaatctgcc aatgaaggag
     2281 atcgctatgc gtaaagcccc ttttactgag catcagatta tcgccgtgat taaatcgctt
     2341 gaatacgggc gaactgttaa agatgtctgc cgggaggcag gcttctctga gccacttact
     2401 acaactggaa gtccagatac ggtggtatgg aatcttctga tattaaaata atcaaagatc
     2461 ttgaggatga gaaccaacgt ctcaaataga tgtttgccga ccccattatt tggtcgggat
     2521 ttcaaaagat gcatggaact ataacggatc tattttcaga ggaatttaac tgaatcagaa
     2581 attaataaat cataattttt agatggctgg tgtttaatgt tgctgcacca tatctatgat
     2641 gcaggaacag tgaatggcta taaattatta gtaaacagaa tgtctatctc tcacctgcat
     2701 ggcggtactt atgaataggc tcccctgttt gggcgtgcac agctagaaca gggtagataa
     2761 aggggccgtg gctgctggcg tcagtagctt gtgcatattc atcatctgtt ttaggcagat
     2821 tgttttcagg gcataaataa aaaatcattg gtgcacgaat gttgaattca gagggttcaa
     2881 gtaaatagtc ccgcttaatt acctcttgtt gcgctttgtt tagtgttgga ttggctataa
     2941 aacgcacggg gattatgacc tcccacgcgg tatctttgcg tggcggggcg ggtgcaggaa
     3001 caatcagttc cggcaagtcc agaaatcgcg ccaaaacaaa gtccttaaac gccgaacttc
     3061 cccagtcata ggctcgcaca tgctgacgcc cgttaacggt gataattacc gtagggctta
     3121 gtgtacgaga tatgccttcc gggttttcca ttgacgcata agtcactttt acttgtcctt
     3181 tctgttcgat tgctgacaaa aaaattgcca gcgtgcggct atgcactgcg cgatggatga
     3241 gcgggatttc ctgcgcgtgg gccgcttgcg gcggttgaaa ttctaccgtc gtacgccaga
     3301 ccagatcgtt gtctcccagc aaaacaggcg taaacgtcgt actggggaaa tacccttttc
     3361 ggccggcccc cttctcaata gcattgcctg gagcgacctc catataggtt cttacatcag
     3421 tacgagcttg ttgcgccgag acgccaaact cttcagcaat catgcgcgaa gtgacctttc
     3481 gttcccacag tagctggcgc tcaatatagc gcaagcgcaa ccattgtgcg tggctgaggg
     3541 gggatttgtg tttctcaaga aactttgagt agtacatatc agttttattc ttatttgcag
     3601 gttaatcaat tgcggtttga tataccgact gagtatagaa ggcgaatgac atcaaaacaa
     3661 ataacattgc tataaataaa aattaagcaa agtttctttg cttaattggc gggcgagtac
     3721 tatagtggct tccgtctcag agggagaatg ttatgtcgct agggtttttg gagcaggctc
     3781 gtaatgaatc aatggcacgt cgtgagctgt ctattcagtc tcacgcctta ctggtcgtgc
     3841 ggacgctgga ttcaatatat agcgcccttc agcgaaatcc gttcgatggt gttgagcttg
     3901 atgcgctggc ggcaatcatg tatctctttg acgccatgct ttattatgct gaccgcgagc
     3961 gccagcctgg aatgacgcgt aaggaagcgg agttatgtct ggttgggcac ctcaatgaag
     4021 cctatccggg acaagaagaa cccgactacg cacaagtggc cagcaagatt tttgcggcga
     4081 tcaaagatcc tttccgcctc cgttattatg atttctcgca acgaacgcat tccgaagagc
     4141 agatagtccg tctggtacgc tatgaccagc tatccggctc agatgatatt ctctggtcgc
     4201 tgactgatga aggaatgttg ttctataccc tgcgcttgga tgaaacgccg atagaacgcg
     4261 ctgcgattct ggcctggcgc acgcttaaaa ccatccgtcg cggagagatc gacaaggcag
     4321 tacaccagat ccaatcaaca cagcggcaac tggagcaata tctgattgga atgcggatga
     4381 aagtgcgtgc cgcccagtct ggtgatttga gtgcatctta tgcacacgat atccgtcccg
     4441 tactcaatga ttcctttgat aaaacccgag aggttttgga acatatccag aactcaatgt
     4501 cggccattac cgaaatgctg gttgaaggaa gttcgaaaat aaatcccgac aaattaaata
     4561 agctacgtag ggcacaggag atttttgctg aggtctgcgc ttttacgcag cgctatcaag
     4621 actgcttact tgatgttaac aaagattttg agcgagctcg tctgaaactc attattccca
     4681 gagataatac cctctttcgg gaaacactgg aaaaaggtgc actcaagccc ttgctgtctc
     4741 attcccccga cactgtcgaa aaggaaatcg atgatctcac cgcacgcctg ttggctcccc
     4801 gaagacagga tgatgatcgg cacattctgt ttgaccttga ggcgctgctg aacctttatc
     4861 tcgatagatt tgaacaggcg cttccacatg agatacagga cctcaccgat cagcctgtag
     4921 ctattgaaac caacctgcga ggtatccctg aatctgtcat taaaaaatgc agcgattggg
     4981 ttagtgaccg attgaagaat tgggggcatg tgagctttag agatgttttt gacgcctatg
     5041 agggcgaaga attatccgaa caagagcaac aggtttgttt actccatatt ggcagcctgg
     5101 cagctaacag cgatcccgca atagaagtgg agatagacgg cgctatcgaa acaacgctct
     5161 ggcgggctaa caatatcaaa ctgttaacgg ttgctccctt ataccccttt gaggagtagt
     5221 tatgtacgac aacgcgcatc aacaaactaa acgctacgcc gagattgcca cgcaggcaat
     5281 tatgaattca ctgggatata aaatggggga tgagctgatt caacgtgctc ttgatctgca
     5341 aggtgatgcc ctgttcacag agattatccg tggcgtcgcg acaggtatga aattgcggta
     5401 tcttgggatt catgagacct acggggtatt actggctgca gatggccgag gtccgttttc
     5461 cccatctttg ggttggctga acaaacgctt gctacctgaa acaatggctg agcaacaggg
     5521 taaggaacgg aaagaacagc gcctgtttgc aggcttaact ctgattgcgt tgctggctga
     5581 tttgtttcca actccagaca ccgttcgtgt cgagttcggt cagagtaacg ccattaatgc
     5641 cgcctcggtg gtagccaggc tcaataatgt tgccgaccag gtgattgccc agcgcaaggc
     5701 cgagggtgac gataccgtag atggcctact tatggcggcc aggtttgtgc aggaagaacg
     5761 tattccccgt atacacgacg aaaaggtagg gcgtgtctca agtattcgct cgcaaacaga
     5821 aatggcaacg cgctggatac aactgctgca ggaggaaggc tttctggccg aagacagcag
     5881 tctgtcggga gagtccgact ggtatccgac ccaaaaactc aaacattatc tgcgccatac
     5941 tggcatcgaa gagacggtgc gctttgtcat gagcatcagt ccagccgtag aagaacggtc
     6001 gcccgattta gctgaaagcg atcttgcagc attagcagat accctggagt ttccttcgct
     6061 caatgaacgt ccagttacaa ctggaaccac agaaacgcaa cgcttgacgg agatttaagt
     6121 atgtggctga ttaaacgggt ttatatggat ggtgcaggcc atgaagaagc tttctacaag
     6181 gacctcacac tggactatat tggcctgtct caagcggtca gtagccctgt tgttgaaggc
     6241 ctgcgctcgc ccgtcaggaa tgcgaaggtg gtgaccagta cttatcagca gcttgtgaat
     6301 gggggtggta agtcgaccta catcgcgctg ctgttatcga tttttgagcc gtccgttagt
     6361 gacttcaccc aatatctggc aaatcatcgc cagagtgaat accactaccg caattacttc
     6421 tataaagaac tctctgttgt tttggttgag atggtcaatg aatcaggaca aacgttatta
     6481 ctaggacatg ctcatcagcg taatggagat gatgttgacc gcacactgtt tatttgtgat
     6541 gcgcccgact ccctgatggg cgcgccgttt gatgaagtcc cttcttattc ccgcgcggaa
     6601 cgcaattctg agctgcgccc ctatgccaat agcctgcgca gttttgatag ctggcttaat
     6661 ctcaaggccg gtagtgaagg cggtatgcat tggcgtaaaa ccaccagaca gaacgagtgg
     6721 cgcgcatttt tactggaaca acaaattaac gttgatctta tcaaaacaat ggtgaccttc
     6781 aactccgaag aaggtggcct gacccgtttt atcgaataca aaactgaaca tgatttcctt
     6841 ggcgcgttct tcgcctgcac catgtctaaa gatgcggctg agcggctgcg ggaactggta
     6901 gcgcatgaag ttgaacgtca gggagagtta gaggggattc gtcgtaacga gtccttcctg
     6961 aaggatgtgc tggctaactg gctggcgttt cttgatccgg ctaaacagct ggaaagcatc
     7021 caggcacagc agcaactgct ggatgatggc ttccgagaag caatgcgcga tctcgcagcc
     7081 tttgtcgggg atgctcaggt tgaacagaca cagcttgaag ataacatgga tagtttgcaa
     7141 acgcatattg gccaggcgat gtctctgcgt gagcagttag gtaagcgcag agcactgctt
     7201 gaatatgatc tcgtgcagca tcggttcaac aaacaaaaag cgatagtcga tgcgcttcaa
     7261 gacgaggccg ataccaatca gcgccagatt gagattactc acgctgctat agactacaaa
     7321 gcctatgcta aggccctgaa tgagtaccgc caggcagatg aagatctgaa ggtattccaa
     7381 agtgaaacgg aaaggccgct acaggtgatt ttcgagcgta ctgctggcga agccttgggc
     7441 acattagagt gggcaatcga cagacatcgc cagcaggagg ctacgcttga ggaagaatta
     7501 gggcagatgg acagggaaag ccatgtcctc agacagtcat acgataaaat acaaaagacg
     7561 ttgggtggtc tggaaacgga gcgtgacgga tataaaagag acaaatttca gggcgaatcg
     7621 caacggaatg aattatgtga agctgggttg ctccagaagg atgaaacacc agtagcagcc
     7681 caataccgac tggaattaac cttacgcgat gcaaagcaga gtgaagaact ggctaataca
     7741 attcttcatc agaaagagca ggcgctggaa gcacagctga atgttgtgcg ggaggcatca
     7801 attgagaaaa gtgaggccca gcaaaaactc attctggctg aacaaaaaca ccagtcggct
     7861 gtggatagtg ctttagtttt agcaaaaaac tatcaaaaac tgccaacgtc aacgcgacat
     7921 gatctggata ttgaagacac gagttggcac agcgagctgt gtggattgca gctcgacaaa
     7981 gtattgaatg cgaacgctca aacgctgaat ggaatgaaag agcgtttact gcaagcccgg
     8041 cacgaactgg acgagttgag ggtgacggga catgaactgg ttgctaagca ggtcaatgag
     8101 gcacttgaag cgttttatgc tgcgggcatt gcacgggata agctatggac atttccacac
     8161 tatttggcct ctgcttacaa cgatgatgca caaaagattg cagcggtgat cgatcagaac
     8221 cctggccgct acctgagtct ggtcgcactc gacaatgtaa cgtttgaaca ggtccataaa
     8281 attagtgaga tcctaccctg gaagaaggca cctattgcta tttacttgct ggatgaagca
     8341 caagatctcc gccgagaaac gtctgaatta gtacaggttg tcctctcaaa ccccgataag
     8401 cacggatata gccaggccgc ttacgaaacc cggctgcaag acctggagaa tactgtagcg
     8461 tcgcaggaag tcagtgtgaa ggctacggag agcgccctta aagtactgga atctttcagg
     8521 aatgactgga gtcggcatta cgaacagtat ggtaagcatt gggcgggact gcttgaccaa
     8581 cgggagctgg ctctattaat actggaaaat gctgatgcag cacttaagca agctgagcat
     8641 gatgagcaaa cactgcgtga cgagaagaga gatgtccagg tgcgtttaaa caacgcccaa
     8701 cagcagaaaa ttcaggcaga aaaagcacat gataaagtga gtctttttgt agaaaaagaa
     8761 tgggcgaagc gtgaaacggc gataatgaaa ctggagcagt tagctgcaca gattcagcga
     8821 agcaagaaca ctctttacac ccagagagag aggctggaac ggctgagcga gctcgagaag
     8881 aacaaacgct ccgagaaaac ccttttaatt atccagatag gtagctggag taatctgtgt
     8941 aacgaaccgt tctattcaga gattacggcg actgaaggta tggaaggacg aagccctgaa
     9001 tatgcccatg agatggctgc tcaggcgcac aaaaaattgc tcgaagcagc caacggtgat
     9061 caagtgaccg ttctcaagca acaactgaaa gatgctgaac aggtacaacg ttacgcctct
     9121 gatcgtttga ccgctcaacc cacctacaat acatatccgg atgaggtaaa agctgcggcc
     9181 atcaatgagc taacggtaat taataacagc atcgtacgcc tcaaagaaca gcaaaagcaa
     9241 ttcacgcaaa atctaattag ctctcaggta actctgcaaa aattttccca aagctgtgag
     9301 gaggctagta aagcacttga cgaggatttt gtctgggaag agaagcccat tgagatagag
     9361 gcaatccaaa cgtctctggc tgaaatcgtc aataaccttt atgcgattga acaccagatc
     9421 aagttcctac tggaacaaaa gacactgctg ttgagaaaga ttgaaaatgt gaaagagcgg
     9481 ctacagaacg ccaaaacagc tcaagcgatg atccagccca ttacgccgag ctctgccgca
     9541 ggtctacctg gtaggatcct gagcatcaca gaatatgctg acgatctggc tgcacaatgc
     9601 caagtgtatg ccggtttaaa tgaaacgcta aaacagcaaa atatgcgcgt ctctacggtc
     9661 tggggaaaat tccgcaaacg catcgaagaa gagttggcca aggatccaga cgcccgagcc
     9721 agtcaggaat acttacggca gttggactca atcgcaactt atcatgaagt gctgggcgat
     9781 ttggagaggg tcggtacagg gatctcgcag gtattcgaag ctgtgcagga cagcctcgag
     9841 caattccaac gttcgattga tctgactgtc acccatctga ctgctcatct ggaaggggcg
     9901 attaagcttc tcaggcgcgc gatgtcggtc aaaattcctg aggattgccc cgtactgcct
     9961 ggtcggccca ttattaaaat gaccgaccgg ttgaatgatg ttgccgctga tctcaggggt
    10021 tttgccagca gccgcttgca gcagtggata gccaggggac agataccccg agcgcccaat
    10081 cgggacgcct tgacagctga tttggttcag tcggtatttg gcgaagggga actggaggtg
    10141 cgtttgctga agacgaatgc cagccgtcca caatggactc ctgtcacccg acttgaaggt
    10201 tcgggtggac agcgccttac atctgccttt ttactgtttg tgacggttgg taaagtacga
    10261 gagtacgata cgggtatttc aagtgcagga ttcttgttag ccgataaccc gctgggtaaa
    10321 tcgaatgcca atgatctaat gcgcattcaa acccaaatgg ccaaagccta taaaatccag
    10381 ctcatctacc tgactggtat ttcggatgaa aacgcccaat caatgtttga taaccatctc
    10441 ttcctgaata aagttcaaaa actgaggcgg cgcgatttgg taaccgttga taaagaacgc
    10501 catgccctgt gggcggcttc tctggctgct aagccacagt tcgatccagt catttaaggt
    10561 tgatatgaga tgagtagcgt aatgatctgg ctatcccgac tggagtcgac agccaataaa
    10621 catcgtcttg ccgctggtaa ggccgcgcgt acagtgaaag ctaatgcggt gctggatgag
    10681 gtattacgcg cacacaacca ggaggccttg agccaggagg tgcctgattt gctgagtcag
    10741 gctgttacac aactgcttga gagggactgg cgggctcagc ctaagcacaa agcgagttgg
    10801 cagcgctact ggcggggtat gagcctgcca agtacttttg tttacgaagg tgccatcgtt
    10861 cctacgaaga caacatcaga aatagcctgg catccctggt tggcccgacg tattacagga
    10921 caacttaccc agccaatcaa ggcacgctta caacagttga atacatatct ttttcgccaa
    10981 accgggggcg acaagtcgct attcccgggc ttgcttggac atcgtgagcg gtctctgctt
    11041 atatttggtg acgaaaaagc tctggactcc ttgccacctg atggctggaa gcatgtcact
    11101 ctgactttgg ctgatatggg cgcgtttcga cgtgcgccgc ctttaccgta tgagtcatct
    11161 cgttgccatg atcttccggc tatcattgtt gaaaactccg atgtgtatta tcggctatgc
    11221 caacttaatc gagcacagac tcgctggtca ctggtcattt atggcgcagg taacaaagtt
    11281 tccggtcagg cggaatgcgt gtcacaactc cttgaggtcg aacaggtaga acaactactt
    11341 tattttggcg atctggatgt tgcgggctta aaaatcgctc atcagttgcg ttgtaagttg
    11401 cttaatgatt acggtatcag cttgcattta gatgaatggc tttataataa actgatctta
    11461 aatggcctga tgacagcgga agggaacgcc aacaacgaac ggtttgattt cgagttacag
    11521 tgtggctgga tgccaaagtt tgttttacga gaaatgaaga ctcttctggc gatccaccag
    11581 cgtctacccc aagaaggtgt ggcttcttta ggtttattta tatagtcata atgtgctttt
    11641 taggtgatat cattcccatt gaatgcatca gcttaatcga ttacagctca ggaaataaaa
    11701 tttttactgt gttaaagttc ctgtaaaatc agttaaaaaa ggaagataaa cgtggcgctg
    11761 tacaatatcg taaataaaga actacatgct ttagaaaaaa cgacctttac tctggaagga
    11821 ttacaggaac gttatgattt acaggaagca atcaaaaaaa atatagatat tattgctcct
    11881 gattgtctgg tgatttccga agagttttct gactgggaag atagccgtcg gcgaattgat
    11941 ttattggcaa tcgataaaca ggcaaattta gtggttatcg aattaaaacg tgatgaaacg
    12001 ggggcgcata tggagttaca ggcactacga tatgcggcga tgatttccac aatgagtttt
    12061 gcgaaagcat gtgaatattt ccagacgtat ttgaaaaaac aaaactgcga tgccgatgcg
    12121 aaagaaaaaa tacttgaatt tgttgaattg gatgaaactg agcttgttga tttcggaaaa
    12181 gatatcagaa ttgttttggc atcatcggat tttagcaagg aactgactac cactgcgatt
    12241 tggttaagag ataagggggt agacattcga tgtgttcgtc tgacgcctta tagatttaat
    12301 gatgatgttc taatcaatgc agagcaaatc ataccggttc ctgaattgga agaatatcaa
    12361 gttaaatttc gtgaaaaacg tgatgaacag ctaattagca gtcagaaaaa agagaaggac
    12421 tatacgtggt atatatataa agataaagaa cttaataaac gtaaattagc gcttgaatta
    12481 ttacgtgatt ggataagaca atttaatcct gcaagttata atgatttaat aaatggatta
    12541 agtgatgact ttaaaaaacg tacggttatg cttatcgatc agataccaga gaagcaaaaa
    12601 agttgctatc atattaatga agatgcgttg attacacttc cctctggtga aattgttgct
    12661 atttctaatc aatggggcat tgttaatatt gaactcttga ttgagtttgt acggcaaaat
    12721 ggttttgttg ttgaaaaagc tgaacaataa attttgatat tgctataaat gtctacattg
    12781 aaaaataggg agaacatttg tagcttttaa catctcaatg cacgagatgg caagattata
    12841 gggacgcgat agttatcaac ctcaccccct aaaaacctct tttcgatgcc actccacaaa
    12901 cccctcaaaa gggtaatttt tacgcacctg tggcaacgca atagttttcc catcaaaatc
    12961 ccagaataat ctctcaacga tccctccgcc gtttaccgca tccgagacca gtacccgcat
    13021 attttcatcc aggccaatcg atcctttatc aaaggcttta tggtgaattg cgcaaagcgc
    13081 caggccatta ggaatttcac aaggaccgcc gtgctgtttc cattttatat gcgccgcttc
    13141 cagcgcgacg gtggtgtcgt cgtggcgcat attaaagcca catatcgcgc actggtagtt
    13201 ataagcgcgt aatacatttt tgcgaaataa cgggtcacgc tgtttacgga tttgctggag
    13261 atcaaaaccc agctcgtcag caatttcttc ctgaatactc tcggtgaagt gcgcttcgag
    13321 tatctgttgc gccagagtat tgatgagttt tttattgccg gttaccaggg cgtagtgctg
    13381 ttcatcgaaa ccgcctgcaa cgtggtattc gttcagttcc ttcactggcg gttgtcggct
    13441 gctccctgct gttgagcaaa gctcggcatt gtgtaattgc cagaatccgt cgccttgtaa
    13501 tcgccagaac ggcatatcag gtcggtactg tgaacgctgt ggcccaaaac gttccagtaa
    13561 attgtgcaga ggttcgtaga tttccaatcc ataatcgaaa aggcgcggat gcccattcag
    13621 gtatcccgct aatacgtata gcagtaacaa tggtttatgc ggcgcgcgct gttcaccctt
    13681 gtgccagatt tttatattgg cgattgcctg ctgtagcgat ttactggaag ccattgatat
    13741 ccctggcagt gaaatagttg cgatcatgct cgcaaatttt ctgctgttca agcctgcaag
    13801 tgaagatata ttaccttgat ctcatttgtt tttttgctgc ctgttctgag aacgtataag
    13861 gacattctcc atgaataaac tccccgatca tcttccgcgg cggtatatca tcgaagctat
    13921 ccatttatgg gatgcgggag aaactcatca gttccagcct gcgcggttgt ataagattga
    13981 atatcatggc cgacgttacc cctcgaaggc gattgctggc atcgcagcga cattgatgac
    14041 ggggacgcaa tttacccctg cggatttcac cggcggaata aaatctaaat gcgtcaaact
    14101 actcatagag cagggatttc atattcatca ggataaagcc gctgcggctc ccatagccga
    14161 cgttctgttc ccggatgagt tgccgtcgca ggagacatac gttgaaggtg cggcgataaa
    14221 ggtgacagta aaccgctatg aacgcgataa aaaggcccga gataaagcgg tcgaacatca
    14281 tggatgccag tgcaacgtat gcggcgttga tctggtgaag atctatggcg atatcgctga
    14341 gggttttatc catatgcatc acctggtgcc gctttcagcc attaaagagg attaccagct
    14401 ggatccagtt aatgatttgc ttcctgtgtg ccctaattgc catgcgatgt tgcaccgacg
    14461 caaaccaccg ttttcgccgg agcagttaaa agcgttgatg gatgcaaatt aatgaatgct
    14521 cacccttcac gccaaaaccg caaaatagcg tcaacaaatt tgtctacaat catcccccga
    14581 ctgaaatgac tgcttcgtgg tgtcgtcgtc cagttctccg gcgttcggta tgttgaatct
    14641 tttccagctt cgttaagatc ttcaaaatag ccgttgaaga tattctggtt aacaccgcca
    14701 gtaacataag ccttgccgtt gtgtacaaat gtcacatgac ccgccatgcc catcggcgcg
    14761 tgcgacatca atttaaccca gctattggtt ttgggg
//
LOCUS       DAMZFT010000121        14745 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.121, whole genome shotgun sequence.
ACCESSION   DAMZFT010000121 DAMZFT010000000
VERSION     DAMZFT010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14745)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14745)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14745
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.121"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..837
                     /locus_tag="PN977_003844"
     CDS             <1..837
                     /locus_tag="PN977_003844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000417848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HDI6043839.1"
                     /translation="YGQFSILTFTPLMGMSDVVTKFLKNPSKSQKVVNMTIYDAEHYT
                     DEQKEQIIASYPEHEREARARGIPTMGSGRIFQIPEETIKCQPFECPDHFYVINAMDF
                     GWDHPQAHIQLWWDKDEDVIYLSRVWKAKQKKATEAWSAVKAWSKNTPTAWPHDGHQH
                     EKGGGAQLKEQYADAGFDMLPDHATWPDGGNAVEPGIAEIRDMMLDGRFKVFNTCEPF
                     FEEFRLYHRDENGKIVKLNDDILSAVRYGYMMRRYAIQMRDIKDPKEIDYSSYNIPCG
                     VG"
     gene            837..3038
                     /locus_tag="PN977_003845"
     CDS             837..3038
                     /locus_tag="PN977_003845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001734492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HDI6043840.1"
                     /translation="MADDRKMTDWHRKVLCNFDNAWSATQDMREQIIEAQRFVRVSGA
                     QWEGSTNAGYSFDEGRFEHYPRFELNKIARECDRIIGEYRQNRISVKFRPKDDKASEA
                     LAEKMNGKFRADYQETSGGEACDNAFDDAVTGGFGCFRMCADYEDEMDPSNEQRRISL
                     LPVYDPATCVFFDQDSKQYDRSDAMWAMEMFSMTPKAFEAEYPDSIAASLSRDDTGTQ
                     YDWSTPDAIYVGRYYEVRIEKVKLTAWRNPVSGETAIYDEEQIKDIVDELTDGAFELI
                     GERTVKKRRVYCGLLSGAEWLEEPKRIPGEHIPLIPVYGRRSFVDNQERIEGHAAKAM
                     DAQRLENLMVSMIADNATQAGGDGIPVVDVDMIPGPLATHWAERNKKRPAFLPMVSLK
                     NKNGDITAQAQVSSYTPPTQMPPALAGLLQYTGTAIQQITGASQLENMPSNVATDTVD
                     SIFNRMDTQSYIYMDNMAKSMRRAGVVWLSMAREVYGSDTPMRIVNEDGSDDVALMTG
                     EVVDRQTGQVIALNDLSQGNYEVTVDVGQSFATRRDATVKSLLSMLALIPPGTPKHDL
                     VSSMILDNMDGEGMDDLKEYNRNQLLLSGVIKPRTPEEQQMVEQAKQQQASQPDPAMV
                     AAQGQLLAGQAELQKAQNEQAAIQVKAFQAQTDAQVAAANVVKILASADSQQKSDIRE
                     ALKLLGQFQQQQGDNARADAELVLKSQAQGHAQRMDINSILQKSTQQQPQQ"
     gene            3129..4022
                     /locus_tag="PN977_003846"
     CDS             3129..4022
                     /locus_tag="PN977_003846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001734493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="scaffolding protein"
                     /protein_id="HDI6043841.1"
                     /translation="MDQMAENTPEVEIETDASEQIPDDVELAEEVETEDGSESSGNDA
                     EEATETDDDESEQEFYFGDEKLDSPTSEDSAEHGLVKHLRKTIKEKDRELKELMRQSQ
                     KSVEQQPVITQPPRMPKLDDEDIGFDEEIYQQRMAKWAEDNGKYQQQEMARKQKEQEL
                     QAAYQERLSKYQQRVKALKVPGYQEAEQAVLEEIPIETQNAILFESEKPEIVVLALGR
                     NAELRKQLAEATNPVAIGRLLERIESKARIMPKAKTTAATIPTVKGSNGAVINNLDKL
                     KAKALETGDWTPYFAAKKAKK"
     gene            4041..5300
                     /locus_tag="PN977_003847"
     CDS             4041..5300
                     /locus_tag="PN977_003847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000013262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coat protein"
                     /protein_id="HDI6043842.1"
                     /translation="MANQLAKDLEIMFENYVEGFEAACVVSRNAKKFRPGDTAMQRAG
                     DVLYRPQHYHMNIEEGLDLSGKTPTALVQRLVPSVFKEPKNILYTLDAREMRDPEHKT
                     EAGRAAGMRLAAQIDSDLISMVTQRATNVVAIPASENGSRGLALWNGAADIDATMTAI
                     GVPQGINRRSFWNPFNYKDLAGELGHRAYAQGATLTAYEKAQIPPVASFDSYKTDISG
                     RLPKGSAESLTVSGQPEHKVEAKDSNGMPVDNRQGTITVSAKGLQVGDAFTIAGVNSV
                     HQITKDTTGQPQVFRVLAVDTAGTTVTISPKILPVENADVASRPYANVDAKPAETAAI
                     TILNKNAAPANLFWADGSVELMYGKLAFPTGQGPQVMTATTEQGATLIMSYAFDHIKG
                     VTTARFTTLYGCSVLVPEYTGIVIAGQ"
     gene            5342..5530
                     /locus_tag="PN977_003848"
     CDS             5342..5530
                     /locus_tag="PN977_003848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001389518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6043843.1"
                     /translation="MARTMLYKPGNMITCGQFTVDYIIVDDEEVKSHLKKGWVKTPEE
                     TATKQKVAKAEEDGENEG"
     gene            5511..5972
                     /locus_tag="PN977_003849"
     CDS             5511..5972
                     /locus_tag="PN977_003849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001597923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head DNA stabilization protein"
                     /protein_id="HDI6043844.1"
                     /translation="MAKTKGDLVLKALRKAGLYSNATLTDADPQAIEDAINDLEDMMA
                     AWQAKGIELGYQFADTENGIMPLPDDDSGIPAWANDGVALKLAVQVCMDNVIQPSDVL
                     LTAADSAYQTICIALTKIPPLERRNDMPRGSGNKSAFTWNRFYIEKDDPST"
     gene            5982..7400
                     /locus_tag="PN977_003850"
     CDS             5982..7400
                     /locus_tag="PN977_003850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="packaged DNA stabilization protein gp10"
                     /protein_id="HDI6043845.1"
                     /translation="MPIQQLPLMKGVGKDFRNADYIDYLPVNMLATPKEILNSSGYLR
                     SFPGIAKRSDVNGVSRGVEYNMAQNAVYRVCGGKLYKGESEVGDVAGSGRVSMAHGRT
                     SQAVGVNGQLVEYRYDGTVKTVSNWPTDSGFTQYELGSVRDITRLRGRYAWSKDGTDS
                     WFITDLEDESHPDRYSAQYRAESQPDGIIGIGTWRDFIVCFGSSTIEYFSLTGATTVG
                     AALYVAQPSLMVQKGIAGTYCKTPFADSYAFISNPATGAPSVYIIGSGQVSPIASASI
                     EKILRSYTADELADGVMESLRFDAHELLIIHLPRHVLVYDASSSANGPQWCVLKTGLY
                     DDVYRAIDFIYEGNQITCGDKLESVTGKLQFDISSQYDKQQEHLLFTPLFKADNARVF
                     DLEVESSTGVAQYADRLFLSATTDGINYGREQMIEQNEPFVYDKRVLWKRVGRIRKNV
                     GFKLRVITKSPVTLSGCQIRIE"
     gene            7400..8101
                     /locus_tag="PN977_003851"
     CDS             7400..8101
                     /locus_tag="PN977_003851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000785531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDI6043846.1"
                     /translation="MADSNLNTPVVVQATRLDTSILPRNIFSQSYLLYVINQGADVGA
                     IAGKANQAGQGAYDAQVKNDEQDVELADHDSRITANTKAINLLEVRLTTAEGKIVVLR
                     SDVDYLLDEVIDIQAHLVTVDQRLDGVESDVSDIKSDYVSKTVTESQSLASPLDVKTS
                     YSVDGIQVIGARNTGWTAATGTPLLGSFNANQSYTVGTTYTQSEVAALATGLQQARQR
                     ILALETALRLHGLID"
     gene            8101..8556
                     /locus_tag="PN977_003852"
     CDS             8101..8556
                     /locus_tag="PN977_003852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2824 family protein"
                     /protein_id="HDI6043847.1"
                     /translation="MITFKPTRNIDLIEAVGNHPDIIAGSNNGDGYDYKPECRYFEVN
                     VHGQFGGIVYYQEIQPLTFDCHAMYLPEIRGFSKEIGLAFWRYILTNTTVQCVTSFAA
                     RKFRHGQIYCAMIGLKRVGTIKKYFKGVDDVTFYSATREELIDFLNHGR"
     gene            8559..9251
                     /locus_tag="PN977_003853"
     CDS             8559..9251
                     /locus_tag="PN977_003853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA transfer protein"
                     /protein_id="HDI6043848.1"
                     /translation="MLYAFKLGRKLCGEEPWCPEKGGKGGSSDKSAKYAAEAQKYAAD
                     LQNQQFNTIMNNLKPFTPLADKYVGSLENLSSLEGQGQALNQYYNSQQYKDLAGQARY
                     QSLAAAEATGGLGSTATGNQLATIAPTLGQQWLSGQMNNYQNLANIGLGALQGQANAG
                     QTYANNMSQISQQSAALAAANANRPSAMQSAIGGGASGAIAGAGLAKLIGSSTPWGAA
                     IGGGLGLLGSLF"
     gene            9262..10677
                     /locus_tag="PN977_003854"
     CDS             9262..10677
                     /locus_tag="PN977_003854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001512997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA transfer protein"
                     /protein_id="HDI6043849.1"
                     /translation="MATWQQGINSGGFLAGIGTQNENAPKASDINATLGLIRENNELA
                     RSGANNIGLTALRGLAGVADIYNQEQQQKAISAFNKVHADAWASGDPSGLFKFAQENP
                     AFVAQAQQAFSGLNEQQRNDMGDLAMRANVALSQGPEAYSKFITDNKDRLNRVGANAD
                     WMIQTGIQNPEQLSHMLTTMSLGALGPEKAFAVQDKMAGREIDRGKLAETIRSNQAGE
                     ALQARGQNLSYQSAMTGHNIAAQRLALDQQEFGFKMQQAQEKAQQLISEAPKLSVNME
                     KGIETAVNNATASSNSANSMSALAQQFRAEKPTTGLFGNAQNMFAKLTGSDTTLRDLR
                     IRQNALVNSQVLKFLPPGPATDKDVEIVRQGAPTDMDNPETVARWLDAMANLERRNAQ
                     FNEFKAEWMSANGNPGQSRNGGQILGLDVKKGESLGSAVKRYMSMNTDAAPAQDSTPS
                     GEPRNQVGSYTSKSGIQFTVE"
     gene            10677..12515
                     /locus_tag="PN977_003855"
     CDS             10677..12515
                     /locus_tag="PN977_003855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001593216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA transfer protein"
                     /protein_id="HDI6043850.1"
                     /translation="MKVTANGKTFTFPDGTSTEDIGAAIDDYFAGQVVQQQTVNQANN
                     APVREEPSLMQQAGDWLTGGQSAGQIAEQAGRGLVNIPFDVLQGGASLINAISQGLGG
                     PKVLDDVYRPVDKPTDPYAQAGETIGGYLVPGVGTAGSMAIGSLAEAANQKGDFAQNA
                     AKNAGVNLAAQGVLSAAAKGIGRGITAIKGDIAPEVAKKIATSESMGVTPMTSDVIPP
                     KNAFTRGLTQDAEGALLGTGSKRAEQYATRSKLVSNYFDRFGEYNPDDVVKSLTTTLR
                     GRKDAAGAVINDVTNKMGNAAVDTTNTMNALNTAIARQERLGTSANQSLLTSLRNLRE
                     ELANPATDLDVTFDLLRQHRTAFRSNVQGDAMVFPNQAKAATNMVENAMSKDLRNAVA
                     KNLGASDAAKYLKANSDYANVYNKVLNKNIANKLNKASSEASPELINTVVLSRKPSDV
                     KRIWSALDDKGKDAMRAAYVSKIAEKAGDSPAKFITEVNKLKSQSGGEIYNTIFSGKH
                     MKELDALHEVLQQTARSDTANVVTQTGQSQANRIRTIGATATLGVSMGIEAGFGAMMR
                     LYESKAARNMLLRLANTKAGTPAYERAINNAANAIRPLFATEATQQ"
     gene            complement(12540..12908)
                     /locus_tag="PN977_003856"
     CDS             complement(12540..12908)
                     /locus_tag="PN977_003856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001334102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6043851.1"
                     /translation="MFLFISFGATAECWVVGDMRGISYSERNNFHPEEDGFSGTFIIK
                     TNGEDASITYSGTDAGGMAYKALSKNSIIGIGANGETQRVIDSWVIHPTGTVLMSKTI
                     SGYGNMDSTKAFVGKVKRKC"
     gene            13047..13466
                     /locus_tag="PN977_003857"
     CDS             13047..13466
                     /locus_tag="PN977_003857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000821346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6043852.1"
                     /translation="MKRIIGVVAGVIMLSGCATIVGDETQLVQVNSNPSGASFKVKDE
                     SGVIVAQGKTPQGVTLAKSDGSYFGKKSYQITMEKDGYEPVTLPIKANANGWYIGGNL
                     VFGGLIGWLAVDPFNGGMYTLKPKEANASLIPSTKQD"
     gene            complement(13491..13733)
                     /locus_tag="PN977_003858"
                     /pseudo
     CDS             complement(13491..13733)
                     /locus_tag="PN977_003858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001749024.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Arc family DNA-binding protein"
     gene            13880..14356
                     /locus_tag="PN977_003859"
                     /pseudo
     CDS             13880..14356
                     /locus_tag="PN977_003859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001386201.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
BASE COUNT         4137 a         3393 c         3742 g         3473 t
ORIGIN      
        1 tacgggcaat tctcaattct gacgtttacc ccgctgatgg ggatgtctga cgttgttacc
       61 aagttcctga agaatcccag caagtcgcag aaagtggtca acatgaccat ctatgacgct
      121 gagcactaca ccgacgagca gaaagagcaa atcatcgcat cctatcccga gcatgagaga
      181 gaggcgcgtg ctcgcggtat tcctacgatg ggtagcggtc gaatcttcca gataccggaa
      241 gagacgatta agtgtcagcc gttcgagtgt cctgatcact tctacgtcat caatgcaatg
      301 gacttcggat gggatcaccc acaggcacat atccagcttt ggtgggataa agacgaggac
      361 gtgatttatc tttctcgcgt ctggaaggcc aaacagaaga aggcgacaga ggcatggagt
      421 gctgttaaag catggagcaa aaacacccct acggcttggc ctcatgacgg gcatcagcac
      481 gaaaagggag gcggcgctca gctcaaggaa caatacgccg acgctgggtt cgacatgttg
      541 ccagatcatg caacatggcc tgatggaggt aatgcggtcg aaccagggat agcagagata
      601 cgcgacatga tgctcgacgg tcgtttcaag gtatttaaca cctgcgagcc attctttgaa
      661 gagtttcgcc tgtatcaccg cgatgagaac gggaagatcg tcaagctaaa tgacgacatc
      721 ctttctgctg ttcgctatgg ctacatgatg aggcgttatg caatacagat gcgagacatc
      781 aaagatccta aagagattga ttactcaagc tacaacatac cttgcggagt tggatgatgg
      841 ctgatgatag aaagatgact gactggcatc gcaaggtgct gtgcaacttt gataatgcct
      901 ggtcagcaac gcaggatatg cgagagcaga ttattgaggc tcaacgtttc gtccgggtgt
      961 ccggcgcaca gtgggaaggc agcacaaacg ctggttactc atttgatgaa ggaaggtttg
     1021 agcattaccc gcgctttgaa ctgaataaga ttgcccgtga atgtgatcgc atcattggcg
     1081 agtatcgaca gaatcgcata agcgttaaat tcaggccgaa ggacgataag gcatcggaag
     1141 cgttagccga aaagatgaac ggcaaattcc gcgctgacta tcaggaaaca tccggtggcg
     1201 aagcgtgtga taacgcattt gatgatgccg taacgggcgg attcggttgt ttccgcatgt
     1261 gtgccgatta cgaagatgaa atggacccaa gtaacgagca gcgacgcatc agccttcttc
     1321 ctgtttacga cccggcgaca tgcgtcttct tcgatcagga cagcaagcaa tatgaccgct
     1381 ctgatgctat gtgggctatg gaaatgttct ccatgacgcc taaagcgttc gaggctgaat
     1441 accctgattc catcgcagca agcctttctc gtgatgacac tggcactcaa tatgactggt
     1501 caacccctga tgctatctat gttggtcgct actacgaagt tcgcatagag aaggtgaagc
     1561 tcacggcatg gcgcaaccct gtcagcggag aaacggcaat ctatgatgaa gagcaaatca
     1621 aagatattgt cgacgagctg accgatggcg cattcgaact gattggtgag cggacagtga
     1681 agaaacgcag agtttattgc ggtcttctgt ctggcgctga atggctggaa gaaccgaagc
     1741 gtattccggg cgaacatatt cctctcatcc cggtatatgg gcgtcgatca tttgttgata
     1801 atcaggagcg aatcgaaggc cacgcagcaa aagcgatgga tgcacagcgt cttgagaacc
     1861 tgatggtttc catgattgca gataacgcca ctcaggctgg cggcgatggc attcctgtag
     1921 ttgatgttga catgattcct ggtcctcttg ccactcattg ggcggagcgc aacaaaaagc
     1981 gcccggcgtt cctgccgatg gtcagtctga aaaacaaaaa cggagatatt actgcgcagg
     2041 ctcaggtcag cagttataca cctccgacac aaatgcctcc tgctcttgct gggctattgc
     2101 agtacaccgg aacggctatt cagcaaatta caggtgcgtc gcagcttgag aacatgccga
     2161 gcaacgtcgc taccgatacc gttgatagca tctttaaccg gatggacacg cagtcctata
     2221 tctacatgga caacatggct aaatccatgc gccgtgctgg cgtcgtgtgg ctttctatgg
     2281 ctcgtgaagt ctatggcagt gatacgccga tgcgtatcgt taatgaggac ggcagcgatg
     2341 acgtggcgct gatgactggt gaagtggttg accgtcagac agggcaggtt atcgcgctta
     2401 atgacctttc gcagggtaac tatgaagtga ctgtcgatgt tggccagtcg ttcgctactc
     2461 gccgtgatgc aacggttaag tcgttacttt ccatgctggc acttatccca ccaggaactc
     2521 cgaagcacga ccttgtatcg tcgatgattc tcgacaatat ggacggcgaa gggatggacg
     2581 acctgaaaga atacaaccgc aatcagttgc ttctgtctgg cgttatcaag ccgagaacgc
     2641 ctgaagaaca gcagatggtt gaacaggcga aacaacaaca ggccagtcag ccagatccgg
     2701 ctatggttgc agcgcaaggt cagcttcttg ctggtcaggc tgaattgcag aaagcgcaga
     2761 acgaacaggc agccattcag gttaaagcat tccaggcaca gactgatgct caggttgcag
     2821 cggcaaatgt tgtgaaaatc ctcgcatctg ccgatagcca gcagaaatct gatatccgcg
     2881 aggctctgaa actgctcgga cagttccagc aacagcaagg agacaatgcc cgtgctgatg
     2941 cagagcttgt cctgaaaagt caggcacagg gtcatgcgca gcgcatggac atcaacagca
     3001 tcctgcaaaa atcaactcag caacaaccac agcagtaatt aacccataac gtgcaatggc
     3061 tgtctttatg aggcctggca ccctattgcc ttccgatggg ctgaacatcg agtaaacagg
     3121 ggtaacaaat ggaccagatg gcagaaaaca caccagaagt tgaaatcgaa accgacgcgt
     3181 cagagcagat tcctgatgat gtcgaactgg ctgaagaagt cgaaacagaa gatggcagtg
     3241 agtcctccgg caatgatgca gaggaagcta ctgaaactga tgacgacgaa tcagagcagg
     3301 aattctactt tggtgatgaa aagctggatt cgccaaccag cgaagatagc gcagagcatg
     3361 gactggtaaa acacctgcgc aagacgatta aagagaaaga tcgcgagctg aaagagctga
     3421 tgcgtcagtc tcagaaatct gtcgagcagc agccggtaat cactcaacca ccgcgaatgc
     3481 caaaactgga tgatgaggac atcggtttcg atgaagaaat ctaccagcaa cgcatggcta
     3541 agtgggcaga ggataacggc aagtaccagc aacaggagat ggctcgcaag cagaaggagc
     3601 aggagcttca ggctgcctat caagagcgat tatccaaata tcagcaacgt gttaaggctc
     3661 tcaaagttcc tggctatcag gaagctgagc aggccgtact cgaggaaatc cccatcgaga
     3721 cacaaaacgc gatcctgttt gagtcagaga agccggaaat cgttgttctg gcgctcggtc
     3781 gcaacgctga actgcgcaag caactggcag aagctaccaa ccccgtagca attggtcgtc
     3841 tgctggaacg tatcgaatcg aaggccagaa tcatgccaaa agcaaaaacc acggcagcca
     3901 caatcccgac agttaagggg agcaacggcg cagtaatcaa taacctcgac aaactgaaag
     3961 ccaaggcgct ggaaactggt gactggacgc cgtatttcgc cgctaaaaag gcaaaaaaat
     4021 aacctatcgg agcattaagc atggctaacc aattagcaaa agaccttgaa atcatgttcg
     4081 aaaactacgt tgaaggcttt gaggctgcct gcgtagtttc ccgtaacgct aaaaaattcc
     4141 gtcccggtga tacagcaatg cagcgagcag gtgatgttct gtatcgtccg cagcattacc
     4201 acatgaacat tgaggaaggc ctagacctca gcggcaaaac gccaacagca ctggttcagc
     4261 gccttgttcc ttctgtgttc aaggagccga aaaacattct gtacactctg gatgcgcgtg
     4321 aaatgcgtga cccggaacat aaaactgaag ctggtcgcgc cgcaggtatg cgccttgctg
     4381 cacagattga ctctgacctg atttccatgg tcacgcagcg tgctactaac gtggtcgcaa
     4441 ttcctgcctc agaaaatggc tcacggggcc ttgccttgtg gaatggtgcg gcagatattg
     4501 atgccaccat gacggcgatt ggtgtacctc agggtatcaa ccgtcgctct ttctggaacc
     4561 ccttcaacta caaagacctt gctggcgagc ttgggcaccg tgcctacgct cagggcgcaa
     4621 ccctgacagc atacgaaaaa gcgcagatcc ctccggttgc ttcctttgat agctacaaga
     4681 ccgatatttc tggtcgatta ccgaaaggaa gcgctgaatc cttgacagta tcaggccaac
     4741 ctgaacacaa ggttgaagcg aaagattcaa atggtatgcc agttgataac cgacagggga
     4801 ctattacggt atctgctaaa ggcttgcagg ttggcgatgc gtttaccatc gcaggtgtga
     4861 attccgtaca ccagatcacc aaagatacca ccgggcagcc gcaggtattc cgcgttctgg
     4921 cagtagacac agcaggaacc accgtaacaa tctctccaaa gattctccct gttgaaaatg
     4981 ccgatgttgc gagccgtcca tatgcaaacg tcgatgccaa gccggcagaa actgcagcaa
     5041 tcaccattct caacaagaac gcagcacctg ctaacctgtt ctgggctgat ggttctgttg
     5101 agctgatgta cggcaaactg gcgttcccga ctggtcaggg tccacaggta atgacagcaa
     5161 ccaccgagca gggcgctacg ctgatcatgt cttacgcctt cgaccacatc aaaggcgtaa
     5221 ccactgctcg tttcaccact ctgtacggtt gctctgtact tgttcctgaa tatacgggca
     5281 tcgttattgc cgggcagtaa ttttggtggg gcttcggccc catttttatt gggagaagac
     5341 aatggcacga acaatgctct ataagccggg caacatgatc acctgtggtc agtttactgt
     5401 cgattacatc attgttgatg acgaagaagt taaatctcac ctgaaaaaag gctgggtaaa
     5461 aactcctgaa gaaaccgcaa cgaagcaaaa agtggctaag gcggaagaag atggcgaaaa
     5521 cgaagggtga tctcgttctt aaggctttac gaaaagccgg gctgtattcc aatgccacgt
     5581 tgacagatgc cgaccctcag gcaattgaag atgccattaa tgacctcgaa gacatgatgg
     5641 cagcatggca ggcgaaaggt atcgagcttg gatatcagtt tgcggataca gaaaatggca
     5701 tcatgccgtt acctgacgat gattcaggta tccctgcatg ggcaaatgat ggcgtcgctt
     5761 tgaaactcgc tgtgcaagtg tgcatggata acgtcattca gccgtcagac gttctcctta
     5821 ccgctgctga cagtgcatat cagacaatct gtatcgcttt aaccaaaata ccaccacttg
     5881 agcggcgaaa tgacatgcct cgcggtagtg gtaacaaaag cgcgtttacg tggaatcggt
     5941 tttacatcga gaaagatgat ccgagtacgt gaggtgaata aatgccgatt cagcaacttc
     6001 cgcttatgaa aggtgtcggc aaagactttc gaaacgccga ctatatcgac tatctgccag
     6061 tgaatatgtt ggctacaccc aaagaaatcc tgaacagcag cggatatctt cgctcattcc
     6121 cgggcattgc caaacgctct gatgtaaacg gcgtatcgcg cggcgtcgag tacaacatgg
     6181 cgcagaatgc tgtttatcgt gtgtgtggcg gcaagctcta caaaggcgaa agcgaagtcg
     6241 gtgacgtcgc cggaagtggt cgtgtatcaa tggcgcatgg tcggacatca caggcggtag
     6301 gcgttaatgg tcaactggtc gagtatcgct atgatggcac ggttaaaacc gtctcaaact
     6361 ggcctacaga cagcggattc acacagtacg agttaggctc agtccgcgac attacgcgct
     6421 tacgtggccg ttatgcgtgg tcaaaagacg gcactgattc atggtttatc actgaccttg
     6481 aagacgaatc acatcctgac cgctacagcg cacaatatcg cgcagaatcg cagccggacg
     6541 gcatcatcgg catcggaaca tggcgagact tcatcgtctg ctttggttca tcgacgattg
     6601 aatatttctc cctgactggc gcaaccaccg ttggtgccgc tttgtatgtc gcacagccat
     6661 cgctgatggt gcagaaaggc attgccggga cttactgcaa aacgccattc gctgattcgt
     6721 atgcgttcat cagcaatccg gcaactggtg cgccatctgt atacatcatc ggctccggtc
     6781 aggtatcacc aatcgccagc gcgagcattg agaaaatact acgctcctac actgctgatg
     6841 aactggctga tggtgtgatg gagtctctga gatttgatgc tcatgagttg ctgattatcc
     6901 accttccgcg tcacgtcctc gtgtacgacg catcttcaag cgccaatggt ccgcaatggt
     6961 gtgtactgaa aacaggcttg tatgacgatg tgtaccgcgc tatcgacttc atttacgaag
     7021 gaaatcagat aacgtgcggc gataagctgg aatcggtgac cgggaaattg cagttcgata
     7081 tcagcagtca gtacgacaag cagcaggaac acctgctgtt tactccgttg ttcaaagcgg
     7141 ataacgccag agttttcgac cttgaagttg aatcgtcaac tggagttgcg cagtatgctg
     7201 accgcctttt tctctctgca accactgacg gcatcaatta cgggcgtgag cagatgattg
     7261 agcagaatga accgttcgtt tacgacaaac gcgttttgtg gaagcgagtt gggcgcatca
     7321 ggaaaaatgt cggcttcaaa ttgcgtgtta tcactaagtc acctgtcact ctgtcaggct
     7381 gccagataag gatcgagtaa tggctgattc gaatctcaat acacctgttg tggttcaggc
     7441 tacacgacta gatacatcaa tccttccacg caatatattc agccagtctt acctgctgta
     7501 tgtcattaat cagggggctg atgtcggcgc aattgccggg aaggcaaatc aggctggtca
     7561 gggcgcttac gatgctcagg tgaaaaacga tgaacaggac gtcgaactgg cagatcacga
     7621 ttcaagaatc accgcaaaca caaaagcgat aaatctcctt gaggtcaggt taacaaccgc
     7681 cgaagggaag atagtcgtac tgcgtagcga tgttgattac ttgctggatg aggttatcga
     7741 tattcaggcg catctggtca ctgttgacca aagactggat ggcgtagaaa gcgatgtatc
     7801 tgacattaag agtgattacg tatcgaaaac cgtaaccgaa tcgcagtctc ttgcgtcacc
     7861 gctggatgta aaaacatcat attcagttga tggaattcag gttattggag caagaaatac
     7921 cggatggact gcagccacag gtacacctct tcttggctca ttcaacgcta accagtcata
     7981 cacagtcggc actacgtaca cacaatccga agtcgcagca ctcgctacag gtttgcagca
     8041 ggcgcggcag cgtattctgg cgcttgaaac ggcacttaga ttacatgggc tgattgactg
     8101 atgattacat tcaaaccaac gcgaaacatc gacctgatag aagccgtagg aaatcaccct
     8161 gacattatcg ccgggagcaa caacggtgat ggatacgact acaaacctga atgccgttac
     8221 tttgaggtta acgtgcacgg gcagttcggc ggaattgttt actatcagga gattcagccg
     8281 ctgacctttg attgccacgc catgtacctg ccagagattc gcggcttcag caaggaaatc
     8341 gggctggctt tctggcgata cattctgact aacaccaccg ttcagtgcgt cacatcgttc
     8401 gccgcacgca aattccgcca cgggcagatt tactgcgcaa tgattggcct taagcgtgtc
     8461 ggaaccatca agaaatactt taaaggcgtg gatgacgtga ctttttacag cgccacacgc
     8521 gaagaactaa tcgacttcct gaatcacggg agatagccat gttatatgca tttaagctgg
     8581 gcagaaaact gtgcggcgag gaaccttggt gccctgaaaa aggcggcaaa ggtggcagtt
     8641 ctgataaaag cgcaaagtat gccgcagaag cccagaagta tgccgcagac ctgcaaaatc
     8701 agcagttcaa caccatcatg aacaacctga agccgtttac tcctctggct gataagtatg
     8761 tcggcagcct cgagaactta tcgtctctgg aagggcaagg tcaggcactt aaccagtatt
     8821 acaactctca gcagtacaaa gatcttgctg gtcaggctcg ctatcagagt ctggcggcag
     8881 cggaagcaac aggtggattg ggttccactg caaccggtaa tcagttagca acaatcgcac
     8941 caacgcttgg tcagcaatgg ctatctggtc agatgaacaa ctaccagaat ctggcaaata
     9001 ttggtcttgg cgcactgcaa ggtcaggcaa acgccgggca aacatatgcc aacaatatga
     9061 gtcagatttc gcagcaaagt gcggctcttg cagcggcaaa tgccaacaga ccatcagcaa
     9121 tgcaatctgc tattggcgga ggtgcgtctg gtgctattgc tggggccgga cttgcgaaat
     9181 taattggttc atcaactccg tggggggctg cgatcggcgg cggtcttggt ctgcttggct
     9241 cgttgtttta aggggtaatc aatggctacg tggcaacagg gtattaattc tggtggtttt
     9301 ctggctggca tcggtacgca aaatgagaat gcgccaaagg caagcgacat taacgcaacg
     9361 cttggtctga tccgcgaaaa caatgaactg gctcgctcag gtgcaaataa cattggtctg
     9421 accgcgttac gtggtctggc tggagttgct gatatttaca atcaggaaca gcaacagaaa
     9481 gctattagtg cgttcaataa ggttcacgct gatgcatggg cttctggtga tccatctgga
     9541 ctatttaagt ttgcccagga aaatccagcg tttgttgcac aggcacaaca ggcgttttcc
     9601 ggtcttaatg agcagcaacg caacgatatg ggcgatttag ccatgagggc taacgtcgct
     9661 ctttcccagg gaccggaagc ctacagtaaa ttcattactg acaacaagga caggttaaat
     9721 cgcgtggggg cgaatgctga ctggatgatt cagacaggta tccagaatcc agagcagcta
     9781 tcacacatgc tgactactat gtctctcggt gcgcttggac cagaaaaggc gtttgctgtt
     9841 caggacaaga tggctggtcg tgaaattgac cgaggcaaac tggcagagac aatccgcagc
     9901 aatcaggctg gagaagcact tcaggcgaga gggcaaaacc tttcctatca gtcagcaatg
     9961 actgggcaca atatcgcagc acaacgcttg gctctggatc agcaagagtt cgggtttaag
    10021 atgcagcaag cacaggaaaa ggctcagcag ttgattagcg aagcacctaa gctgtcagta
    10081 aacatggaaa aaggcatcga gacggctgta aacaatgcta cagcatcatc aaactcagcc
    10141 aattctatga gtgcgcttgc tcaacagttc agagcagaaa aaccaacgac aggtttgttc
    10201 ggtaacgcac agaacatgtt cgcaaaactt accggaagcg atacaacatt gcgtgatttg
    10261 cgcatccgcc aaaatgccct tgttaacagt caggttctta aattcctacc tcccggcccc
    10321 gcaacggata aagacgttga gatcgttcga cagggtgctc caactgacat ggataaccct
    10381 gagacggtag caagatggct tgatgcaatg gcaaaccttg agcgacgaaa cgcgcagttt
    10441 aatgagttta aagccgagtg gatgagcgcg aatggcaacc ctggacaatc gcgtaatggc
    10501 ggtcagatat tggggttgga tgttaaaaaa ggtgaatcat tggggagtgc cgttaagcgg
    10561 tatatgtcaa tgaatactga cgcagcgcca gcacaagatt cgacaccttc aggagaacca
    10621 cggaatcagg ttggatcata tacctcaaaa tcaggcattc aatttacggt ggaatgatga
    10681 aagtaactgc aaacggtaag acatttacct tccctgatgg tacgagcacg gaagatattg
    10741 gagccgccat tgatgattat tttgctggtc aggttgttca gcaacaaaca gttaatcagg
    10801 ccaataatgc accagtacgt gaagaaccat cattgatgca acaagctggc gattggctca
    10861 ctggtggtca aagtgcaggg caaattgcag aacaggctgg tcgtggtctg gtaaacatac
    10921 catttgacgt attgcagggt ggcgcaagtc tgattaatgc aatcagtcag gggcttggtg
    10981 ggccaaaagt tttggatgat gtttatcgtc cagtagacaa accgacagac ccatacgcac
    11041 aagccggtga aacaattggt gggtatttag ttccaggagt tggaacggca ggaagcatgg
    11101 ctattggatc gctggcagag gccgcaaatc agaaaggcga tttcgcacaa aatgcagcta
    11161 aaaatgccgg agttaacctt gccgctcagg gtgttctttc cgcagcagca aagggaatag
    11221 ggcgtggaat aacggctata aaaggtgata ttgcgccaga agtggcgaag aaaattgcca
    11281 catcagaatc gatgggcgtg acaccaatga catctgatgt tatcccgccg aaaaatgctt
    11341 tcactcgcgg ccttactcag gatgccgagg gggctttgct cgggacgggc tcaaagcgag
    11401 cagagcaata tgcaacgcgg agtaagctgg taagcaatta ttttgaccgt tttggtgagt
    11461 acaaccctga tgatgtggtg aaatctctga ccaccacgtt aagggggcgg aaggatgccg
    11521 ctggcgctgt tatcaatgac gtcaccaata aaatgggtaa tgccgcagtt gataccacaa
    11581 ataccatgaa tgctctgaat acagcgatcg caagacagga acggcttggg acgtctgcca
    11641 atcaaagcct gcttacatcc ttgcgtaacc tacgtgaaga attagcaaac cctgcaactg
    11701 atttggatgt tacgtttgat ctcttgcgtc agcacagaac agcatttaga tctaatgttc
    11761 agggagatgc tatggtcttc cccaaccagg caaaagcagc taccaatatg gtagagaatg
    11821 caatgtcaaa agaccttcgt aacgcagttg ctaaaaacct cggtgcatca gacgcagcaa
    11881 aataccttaa agcaaattcc gattatgcaa acgtttataa taaggtgctt aataaaaaca
    11941 ttgccaacaa gctcaacaag gcaagcagtg aagccagtcc tgaacttata aataccgttg
    12001 tattaagcag aaaaccatct gacgtgaaac gaatctggag cgcactggat gataagggga
    12061 aagatgctat gcgtgcagct tacgtcagca aaatagcgga aaaggccggg gactctccag
    12121 ccaagttcat cactgaagtt aataagctga aatctcagtc aggcggtgaa atttacaaca
    12181 ctattttttc tggaaagcac atgaaagagc ttgatgctct tcatgaagtt ctacagcaaa
    12241 cagcaaggtc agacaccgca aatgtagtaa ctcagacggg gcaatcgcaa gccaacagga
    12301 taaggacgat tggcgcaact gcgacccttg gcgtatcaat ggggattgag gctggttttg
    12361 gtgcaatgat gcgcttgtat gagtccaaag cagcaaggaa tatgcttctt cgtttggcaa
    12421 acaccaaagc aggaacacca gcctatgaaa gagcgataaa taacgctgcg aatgccatca
    12481 gaccgctgtt tgctactgag gcaacacagc agtaacgtat gggaaattgg attcaatcgc
    12541 taacattttc tttttacttt tccaacaaaa gctttggttg aatccatatt tccataaccg
    12601 gaaatggttt ttgacattaa aactgttcca gtaggatgta ttacccatga gtcgataacg
    12661 cgttgagttt cgccattcgc gccgattcct atgatggagt ttttagacaa tgctttgtaa
    12721 gccatgccgc ccgcatctgt cccagaatat gtgatgctgg catcttcacc gtttgtctta
    12781 atgatgaatg ttccactaaa accatcttct tccggatgga aattatttcg ttctgaatag
    12841 cttattccgc gcatatctcc aacgacccag cactctgctg tagccccaaa agatatgaat
    12901 aagaacatag cagtaagaaa ttgcttcacg ccaacctcct tagttttgag caggatacca
    12961 tgaaaaaagt aaacatcttt tgcctacttc acatttgaat ggtttgtcat taggatgttt
    13021 ccggtttttt aaatatggaa attgatatga agaggattat tggcgtcgtt gctggcgtta
    13081 tcatgttatc tgggtgcgca actattgttg gtgacgaaac acagcttgtg caagtgaaca
    13141 gcaatccttc cggtgcgagc tttaaggtaa aagacgaatc aggcgtgatt gttgcgcaag
    13201 gtaagacccc gcaaggagta actcttgcca agtcagatgg tagttatttt ggcaaaaaga
    13261 gctaccagat cactatggag aaggatgggt acgaaccagt taccctgcca atcaaagcca
    13321 atgctaatgg ttggtatatt ggtggaaacc ttgtgtttgg tgggttaatt ggttggcttg
    13381 ctgtagatcc atttaatggt gggatgtata ccttgaagcc taaagaggca aatgcatccc
    13441 ttataccgtc cacaaagcaa gactaataaa tggaacccac catcaggtgg gtttttgtac
    13501 aaatccttca gcgtatcaaa caccatcttc ttaacaagct ctgactgctc atcagcgatg
    13561 cgttccgcat cgtctcgata gcctgaaatt ttggatggct ttgatacagc atcagtcact
    13621 atctgaacta attctgaatt aagagagcgg ccattggatt tggctcgctg ttttagtttt
    13681 tcctttaatt cgtaaggtag ccgcagatta aattgcgggt catctcttcc cattcttgat
    13741 gcctcgcttt tgtgagtgga tcggcatctt attatctgct ggttgcatcc tcaataagac
    13801 cacagtggtc tctttgtttg attaataatg catcactgtg gcaatgctgc ggcgattcct
    13861 tgtatctgga gcaaattaaa tgacagacat tacagccaat gttgtagtga gtatgccttc
    13921 gcaactcttc actatggcgc gttcttttaa agccgtagcc aatggcaaaa tttatatcgg
    13981 taaaattgat actgcccctg taaatcctga aaaccagatt caggtttatg tagagaacga
    14041 agatggatct cacgttcctg tttcgcaacc aatcatcatt aacgctgctg gatatccggt
    14101 atataacgga cagattgcca aattcgttac tgtacagggc cattctatgg ctgtttatga
    14161 tgcgtacggt gtgcagcagt tctattttcc gaatgtgctg aagtatgacc cggatcagtt
    14221 aagacaagag ttagcaacag agggtaatga aatacttgtt gacgactccc gtattgcagt
    14281 taaaagtgtt catgtaaatt caaaacttag aacacagcac aataaaaacg cagaaataat
    14341 cacgccaaat gattttatat caaattatga catgatatct gcagctatta actctggaga
    14401 gagtgccgca gtatttaatt ccaggtatgc attcacaata acagttgggg aatatggtaa
    14461 ttttaaatct attagtgatg ctctaaactc agctgttgca ctgcgtccac tatggaaaaa
    14521 tggcattgat ttttgcataa ttaaaattct ttccggtttt gtgctcaatg agcaaataga
    14581 aatttcagca ggatgtgact tatcatggat taaaataata tcagaagact cagtggtttt
    14641 ctctgatacc agtttgttta ctaaaactgt taggacgtat tacgaataca aatatttatt
    14701 ttacatttgc gatgcagcaa aaagtcctgt atttgctatt cagat
//
LOCUS       DAMZFT010000122        14743 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.122, whole genome shotgun sequence.
ACCESSION   DAMZFT010000122 DAMZFT010000000
VERSION     DAMZFT010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14743)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14743)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14743
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.122"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..191
                     /locus_tag="PN977_003860"
     CDS             <1..191
                     /locus_tag="PN977_003860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HDI6043853.1"
                     /translation="ELAHSLGLKTIAEGVETEEQVNWLRKRGVRYCQGWFFAKAMPPQ
                     VFMQWMEQLPARELTRGQ"
     gene            complement(194..517)
                     /gene="soxS"
                     /locus_tag="PN977_003861"
     CDS             complement(194..517)
                     /gene="soxS"
                     /locus_tag="PN977_003861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide response transcriptional regulator
                     SoxS"
                     /protein_id="HDI6043854.1"
                     /translation="MSHQKIIQDLIAWIDEHIDQPLNIDVVAKKSGYSKWYLQRMFRT
                     VTHQTLGDYIRQRRLLLAAVELRTTERPIFDIAMDLGYVSQQTFSRVFRRQFDRTPSD
                     YRHRL"
     gene            603..1067
                     /gene="soxR"
                     /locus_tag="PN977_003862"
     CDS             603..1067
                     /gene="soxR"
                     /locus_tag="PN977_003862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensitive transcriptional activator SoxR"
                     /protein_id="HDI6043855.1"
                     /translation="MEKKLPRIKALLTPGEVAKRSGVAVSALHFYESKGLISSIRNSG
                     NQRRYKRDVLRYVAIIKIAQRIGIPLATIREAFGVLPEGHTLSAKEWKQLSSQWREEL
                     DRRIHTLVALRDELDGCIGCGCLSRSDCPLRNPGDRLGEEGTGARLLEDEQN"
     gene            1612..2961
                     /gene="ghxP"
                     /locus_tag="PN977_003863"
                     /gene_synonym="yjcD"
     CDS             1612..2961
                     /gene="ghxP"
                     /locus_tag="PN977_003863"
                     /gene_synonym="yjcD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanine/hypoxanthine transporter GhxP"
                     /protein_id="HDI6043856.1"
                     /translation="MSTPSARTGGSLDAWFKISQRGSTVRQEVVAGLTTFLAMVYSVI
                     VVPGMLGKAGFPPAAVFVATCLVAGLGSIVMGLWANLPLAIGCAISLTAFTAFSLVLG
                     QHISVPVALGAVFLMGVLFTVISATGIRSWILRNLPHGVAHGTGIGIGLFLLLIAANG
                     VGLVIKNPLDGLPVALGDFTTFPVMMSLVGLAVIIGLEKLKVPGGILLTIIGISIVGL
                     IFDPNVHFSGVFAMPSLSDENGNSLIGSLDIMGALNPVVLPSVLALVMTAVFDATGTI
                     RAVAGQANLLDKDGQIIDGGKALTTDSMSSVFSGLVGAAPAAVYIESAAGTAAGGKTG
                     LTAITVGVLFLLILFLSPLSYLVPGYATAPALMYVGLLMLSNVAKIDFADFVDAMAGL
                     VTAVFIVLTCNIVTGIMIGFATLVIGRLVSGEWRKLNIGTVVIAVALVTFYAGGWAI"
     gene            3112..4761
                     /locus_tag="PN977_003864"
     CDS             3112..4761
                     /locus_tag="PN977_003864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter"
                     /protein_id="HDI6043857.1"
                     /translation="MEIFFTILIMTLVVSLSGVVTRVMPFQIPLPLMQIAIGALLAWP
                     TFGLHVEFDPELFLVLFIPPLLFADGWKTPTREFLEHGREIFGLALALVVVTVVGIGF
                     LIYWVVPGIPLIPAFALAAVLSPTDAVALSGIVGEGRIPKKIMGILQGEALMNDASGL
                     VSLKFAVAVAMGTMIFTVGGATVEFMKVAIGGILAGFVVSWLYGRSLRFLSRWGGDEP
                     ATQIVLLFLLPFASYLIAEHIGVSGILAAVAAGMTITRSGVMRRAPLAMRLRANSTWA
                     MLEFVFNGMVFLLLGLQLPGILETSLMAAEIDPNVEIWMLFTDIILIYAALMLVRFGW
                     LWTMKKFSNRFLKKKPMEFGSWTTREILIASFAGVRGAITLAGVLSIPLLLPDGNVFP
                     ARYELVFLAAGVILFSLFVGVVMLPILLQHIEVADHSQQLKEERIARAATAEVAIVAI
                     QKMEERLAADTEENIDNQLLTEVSSRVIGNLRRRADGRNDVESSILEENLERRFRLAA
                     LRSERAELYHLRATREISNETLQKLLHDLDLLEALLIEENQ"
     gene            complement(4843..6492)
                     /gene="actP"
                     /locus_tag="PN977_003865"
     CDS             complement(4843..6492)
                     /gene="actP"
                     /locus_tag="PN977_003865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation/acetate symporter ActP"
                     /protein_id="HDI6043858.1"
                     /translation="MKRVLTALAATLPFAANAADAISGAVERQPTNWQAIIMFLIFVV
                     FTLGITYWASKRVRSRNDYYTAGGNITGFQNGLAIAGDYMSAASFLGISALVFTSGYD
                     GLIYSLGFLVGWPIILFLIAERLRNLGRYTFADVASYRLKQGPIRILSACGSLVVVAL
                     YLIAQMVGAGKLIELLFGLNYHIAVVLVGVLMMMYVLFGGMLATTWVQIIKAVLLLFG
                     ASFMAFMVMKHVGFSFNNLFSEAMAVHPKGVDIMKPGGLVKDPISALSLGLGLMFGTA
                     GLPHILMRFFTVSDAREARKSVFYATGFMGYFYILTFIIGFGAIMLVGANPEYKDAAG
                     HLIGGNNMAAVHLANAVGGNLFLGFISAVAFATILAVVAGLTLAGASAVSHDLYANVF
                     KKGATEREELRVSKITVLILGVIAIILGVLFENQNIAFMVGLAFAIAASCNFPIILLS
                     MYWSKLTTRGAMLGGWLGLITAVVLMILGPTIWVQILGHEKAIFPYEYPALFSISVAF
                     LGIWFFSATDNSAEGARERELFRAQFIRSQTGFGVEQGRAH"
     gene            complement(6489..6803)
                     /locus_tag="PN977_003866"
     CDS             complement(6489..6803)
                     /locus_tag="PN977_003866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF485 domain-containing protein"
                     /protein_id="HDI6043859.1"
                     /translation="MNGTIYQRIEDNAHFRELVEKRQRFATILSIIMLAVYIGFILLI
                     AFAPGWLGTPLNPNTSVTRGIPIGVGVIVISFVLTGIYIWRANGEFDRLNNEVLHEVQ
                     AS"
     gene            complement(7016..8974)
                     /gene="acs"
                     /locus_tag="PN977_003867"
     CDS             complement(7016..8974)
                     /gene="acs"
                     /locus_tag="PN977_003867"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HDI6043860.1"
                     /translation="MSQIHKHTIPANIADRCLINPQQYEAMYQQSINAPDTFWGEQGK
                     ILDWIKPYQKVKNTSFAPGNVSIKWYEDGTLNLAANCLDRHLQENGDRTAIIWEGDDA
                     SQSKHISYKELHRDVCRFANTLLELGIKKGDVVAIYMPMVPEAAVAMLACARIGAVHS
                     VIFGGFSPEAVAGRIIDSNSRLVITSDEGVRAGRSIPLKKNVDDALKNPNVTSVEHVV
                     VLKRTGGKIDWQEGRDLWWHDLVEQASDQHQAEEMNAEDPLFILYTSGSTGKPKGVLH
                     TTGGYLVYAALTFKYVFDYHPGDIYWCTADVGWVTGHSYLLYGPLACGATTLMFEGVP
                     NWPTPARMAQVVDKHQVNILYTAPTAIRALMAEGDKAIEGTDRSSLRILGSVGEPINP
                     EAWEWYWKKIGNEKCPVVDTWWQTETGGFMITPLPGATELKAGSATRPFFGVQPALVD
                     NEGHPLEGAAEGSLVITDSWPGQARTLFGDHERFEQTYFSTFKNMYFSGDGARRDEDG
                     YYWITGRVDDVLNVSGHRLGTAEIESALVAHPKIAEAAVVGIPHNIKGQAIYAYVTLN
                     HGEEPSPELYAEVRNWVRKEIGPLATPDVLHWTDSLPKTRSGKIMRRILRKIAAGDTS
                     NLGDTSTLADPGVVEKLLEEKQAIAMPS"
     gene            9367..10803
                     /gene="nrfA"
                     /locus_tag="PN977_003868"
     CDS             9367..10803
                     /gene="nrfA"
                     /locus_tag="PN977_003868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-forming nitrite reductase cytochrome
                     c552 subunit"
                     /protein_id="HDI6043861.1"
                     /translation="MTRIKINARRIFSLLIPFFFFTSVHAEQTAAPAKPVTVEAKNET
                     FAPQHPDQYLSWKATSEQSERVDALAEDPRLVILWAGYPFSRDYNKPRGHAFAVTDVR
                     ETLRTGAPKNAEDGPLPMACWSCKSPDVARLIQKDGEDGYFHGKWARGGPEIVNNLGC
                     ADCHNTASPEFAKGKPELTLSRPYAARAMEAIGKPFEKAGRFDQQSMVCGQCHVEYYF
                     DGKNKAVKFPWDDGMKVENMEQYYDKIAFSDWTNSLSKTPMLKAQHPEYETWTAGIHG
                     KNNVTCIDCHMPKVQNAEGKLYTDHKIGNPFDNFAQTCANCHTQDKAALQKVVAERKQ
                     SINDLKIKVEDQLVHAHFEAKAALDAGATEAEMKPIQDDIRHAQWRWDLAIASHGIHM
                     HAPEEGLRMLGTAMDKAADARTKLARLLATKGITHEIQIPDISTKEKAQQAIGLNMEQ
                     IKAEKQDFIKTVIPQWEEQARKNGLLSQ"
     gene            10849..11415
                     /gene="nrfB"
                     /locus_tag="PN977_003869"
     CDS             10849..11415
                     /gene="nrfB"
                     /locus_tag="PN977_003869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418495.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase pentaheme
                     subunit"
                     /protein_id="HDI6043862.1"
                     /translation="MSVLRSLLTAGVLASGLLWSLNGITATPAAQASGDRYEVTQQRN
                     PDAACLDCHKPDTEGMHGKHASVINPNNKLPVTCTNCHGQPSPQHREGVKDVMRFNEP
                     MYKVGEQNSVCMSCHLPEQLQKAFWPHDVHVTKVACASCHSLHPQQDTMQTLSDKGRI
                     KICVDCHSDQRTNPNFNPASVPLLKEQP"
     gene            11412..12083
                     /gene="nrfC"
                     /locus_tag="PN977_003870"
     CDS             11412..12083
                     /gene="nrfC"
                     /locus_tag="PN977_003870"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase Fe-S protein"
                     /protein_id="HDI6043863.1"
                     /translation="MTWSRRQFLTGVGVLAAVSGTAGRVVAKTLNINGVRYGMVHDES
                     LCIGCTACMDACRKVNKVPEGVSRLTIIRSEPQGEFPDVKYRFFRKSCQHCDRAPCVD
                     VCPTGASFRDAASGIVDVNPDLCVGCQYCIAACPYRVRFIHPVTKTADKCDFCRKTNL
                     QAGKLPACVEACPTKALTFGNLDDPNSEISQLLHQKPTYRYKLALGTKPKLYRVPFKY
                     GEVSQ"
     gene            12080..13036
                     /gene="nrfD"
                     /locus_tag="PN977_003871"
     CDS             12080..13036
                     /gene="nrfD"
                     /locus_tag="PN977_003871"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase subunit NrfD"
                     /protein_id="HDI6043864.1"
                     /translation="MTQTSAFHFESLVWDWPIAIYLFLIGISAGLVTLAVLLRRFYPQ
                     AGGADSTLLRTTLIVGPGAVILGLLILVFHLTRPWTFWKLMFHYSFTSVMSMGVMLFQ
                     LYMVVLVLWLAKIFEHDLLALQQRWLPKLGIVQKVLSLLTPVHRGLETLMLVLAVLLG
                     AYTGFLLSALKSYPFLNNPILPVLFLFSGISSGAAVALIAMAIRQRSNPHSTEAHFVH
                     RMEIPVVWGEIFLLVAFFVGLALGDDGKVRALVAALGGGFWTWWFWLGVAGLGLIVPM
                     LLKPWVNRSSGIPAVLAACGASLVGVLMLRFFILYAGQLTVA"
     gene            13056..>14743
                     /locus_tag="PN977_003872"
     CDS             13056..>14743
                     /locus_tag="PN977_003872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014640253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HDI6043865.1"
                     /translation="MDVFLPEVGFLALLLSLGVNVLTPLTAFAGVRLRWPAMMRLTCI
                     GILAQFALLLLAFGVLTYCFLISDFSVIYVAQHSYSLLSWELKLAAVWGGHEGSLLLW
                     VLLLSAWSALFAWHYRQQTDPLFPLTLAVLSLMLAALLLFVVLWSDPFVRIFPPAIEG
                     RDLNPMLQHPGLIFHPPLLYLGYGGLMVAASVALASLLRGEFDGTCARICWRWALPGW
                     GALTAGIILGSWWAYCELGWGGWWFWDPVENASLLPWLSATALLHSLSLTRQRGIFRH
                     WSLLLAIVTLMLSLLGTLIVRSGILVSVHAFALDNVRAVPLFSLFALISLASLTLYGW
                     RARDGGPAVRFSGLSREMLILATLLLFCAVLLIVLVGTLYPMIYGLLGWGRLSVGAPY
                     FNRATLPFGLLMLVVIVLATFVSGKRVQLPALLAHAGVLLFAAGIVVSSVSRQEISLN
                     LQPGQQVTLAGYAFRFERLDLQARGNYTSEKAIVALFDHQQRIGELTPERRFYEARRQ
                     QMMEPSIRWNGIHDWYAVMGEKTGPDRYAFRLYVQSGVRWIWGGGLLMIAGALL"
BASE COUNT         3140 a         3823 c         4200 g         3580 t
ORIGIN      
        1 tcgagctggc gcacagtctg gggttaaaaa cgatcgctga aggcgtcgaa actgaggaac
       61 aggttaactg gctgcgcaaa cgcggcgtgc gctattgcca gggatggttc tttgcgaagg
      121 cgatgccgcc gcaggtgttt atgcaatgga tggagcaatt acccgcgcgg gagttaacgc
      181 gcgggcaata aaactacagg cggtggcgat aatcgcttgg agtgcgatca aactgccgac
      241 ggaaaacgcg ggagaaggtc tgctgcgaga cataacccag gtccattgcg atatcaaaaa
      301 tcggacgctc ggtggtgcgc aactcaacgg cggccagcaa caggcggcgt tggcgaatgt
      361 aatcaccaag tgtctgatgc gtcaccgtgc ggaacatccg ctgtaagtac cactttgaat
      421 agcctgattt ttttgcgacg acatcaatgt taagcggctg gtcaatatgc tcgtcaatcc
      481 atgcgataag atcctgaata attttctgat gggacataaa tctgcctctt ttcagtgttc
      541 agttcgttaa ttcatctgtt ggggagtata attcctcaag ttaacttgag gtaaagcgat
      601 ttatggaaaa gaaattaccc cgcattaaag cgctgctaac ccccggcgaa gtggcaaaac
      661 gcagcggtgt ggcggtatcg gcgctgcatt tctatgaaag caaagggttg attagcagta
      721 tccgtaatag tggcaatcag cggcgatata aacgtgatgt gttgcgatat gtcgcaatca
      781 tcaaaattgc tcagcgtatt ggcattccgc tggcgaccat tcgtgaagca tttggcgtgt
      841 tgcccgaagg gcatacatta agtgcgaaag agtggaaaca gctttcgtcc caatggcgag
      901 aagagttgga tcggcgcatt cataccttag tggcgctgcg tgacgaactg gacggatgta
      961 ttggttgtgg ctgcctttcg cgcagtgatt gcccattgcg taacccgggc gaccgcttag
     1021 gagaagaagg cacaggcgca cgcttgctgg aagatgaaca aaactaaagc gccacaaggg
     1081 cgctttagtt tgttttccgg tctttgtctt tctctctatc ccgctggtac acaggagggt
     1141 ttcccccgac gtcaacacac ctcattcgag cacgtggtgg aggttccggt tggtgttgat
     1201 gctttaattg tatgtcaccg gcgtttcttc gccagtgtaa aagtacactt tttagccgca
     1261 atatttttgt catctcaggc gattttttat cgcaatcctg aacggtatac ggttcgataa
     1321 cgctgcaatc ttgcgcatcg acgataacgt ttgcgcatca attgcctggt ttttcatcgt
     1381 caagacaata aaagagaaaa aagcagcaaa cttcggttga aaaagccgct atgatcgccg
     1441 gataatcgtt tgctttttta ccacccgttt tgtatgcgcg gagctaaacg tttgcttttt
     1501 tgcgacgcac caatttgtcg caaacctgga gcaggaagat aacgtttcgc tggcagggga
     1561 ttgtccgcca cgcatcttga cgaaaattaa actctcaggg gatgttttct tatgtctacg
     1621 ccatcagcgc gtaccggcgg ttcactcgac gcctggttta aaatttcaca acgtggaagc
     1681 actgtccgtc aggaagtggt tgccgggtta acaacgtttc tggcgatggt ctactcggtt
     1741 atcgtcgttc caggcatgtt gggtaaagcg ggcttcccgc ctgcggcagt tttcgttgcg
     1801 acctgtctgg ttgccggact cggttctatc gtgatgggtc tgtgggctaa tctgccgttg
     1861 gcgattggtt gcgccatctc cctgacggcg tttaccgcat tcagtctggt gctggggcaa
     1921 catattagcg tacctgtcgc gctgggtgcc gtgttcctga tgggtgtgct gtttacggtc
     1981 atttctgcca cgggtatccg tagctggatt ttgcgcaact tgccgcacgg tgtggcgcac
     2041 ggcacgggga tcggtatcgg tctgttcctg ctgctgattg ctgcgaatgg tgtcggtctg
     2101 gtgattaaaa acccgcttga tggtctgccc gttgcgctgg gtgatttcac gacctttccg
     2161 gtgatgatgt cactggtagg tctggcggtg atcatcggcc tggaaaaact gaaagtccct
     2221 ggtggcattc tgctgaccat tatcggtatc tcaattgtcg gtttgatctt cgatcctaac
     2281 gtccatttct ccggcgtttt cgctatgcct tcattgagcg atgaaaacgg caattcactg
     2341 attggcagcc tggatattat gggcgcgctg aatcctgtag tcctgccaag cgttctggcg
     2401 ctggtgatga cggcagtatt tgatgccacc ggaactatcc gcgccgtcgc cggtcaggcg
     2461 aacctgctgg ataaagacgg gcagatcatc gacggcggga aagcactgac cactgactcc
     2521 atgagcagcg ttttctctgg cctggtgggt gcagctccgg cagcggtata catcgagtct
     2581 gcggcgggta cggcggcggg cggtaaaacc gggttgacgg ctatcaccgt tggcgtgctg
     2641 ttcctgctga ttctgttcct ctctccgctc tcttacctcg ttccggggta cgcaacggct
     2701 ccggcgttga tgtacgttgg cctgctgatg ctgagcaacg tggcgaaaat cgactttgct
     2761 gattttgttg atgcgatggc gggtctggtt acggcggtat tcatcgtgct gacctgtaac
     2821 atcgtaacag gcatcatgat cggcttcgcg actctggtga ttggtcgtct ggtttccggc
     2881 gaatggcgca agttgaacat cggtacggtc gttattgccg tggcgctggt gaccttctat
     2941 gcgggtggct gggctatcta atcttacata ggattctgaa aacgggtggc aatggctgcc
     3001 cgtttttatt ttctccgcac attgtgtgag cttttgcgat attctgaaaa aatgagaatt
     3061 caggcataac gcctttctca gatcataaaa aacatcgcaa acagggaacg catggaaatc
     3121 ttcttcacca tactgataat gaccctcgtg gtctcgctgt ccggggtggt cactcgtgtc
     3181 atgccctttc agatcccgct tccgcttatg caaatcgcca tcggtgcgct actggcgtgg
     3241 ccgacgtttg gtttgcatgt ggagtttgat cctgaactct ttttagtctt gtttatcccg
     3301 ccgttgctgt tcgctgatgg ctggaaaacg ccgacccgtg aatttctcga acatgggcga
     3361 gagattttcg gcctcgcgct ggcgctggtg gtggtcaccg tggtcggcat tggcttcctt
     3421 atttactggg tggtgccggg cattccgctg atccccgcct ttgcgctggc ggcggtgctt
     3481 tctccgaccg atgctgtggc gctctccggg attgttggcg aagggcgcat cccgaaaaaa
     3541 atcatgggca ttttgcaggg cgaagcgttg atgaatgacg cctccggtct ggtgtcgttg
     3601 aagtttgccg tggcagtggc gatggggacg atgatcttca ccgtcggtgg tgcgacggtc
     3661 gaatttatga aagtggccat tggcggtatt ctcgccggtt ttgtggtgag ctggctgtac
     3721 ggtcgctcgc tgcgattcct cagccgctgg ggcggtgatg aacccgcgac gcagattgtt
     3781 ctactgttct tgctgccatt cgcttcttat ctgattgccg aacatattgg cgtttccggc
     3841 atcctcgctg cggttgccgc cgggatgacc atcacccgct ccggtgtgat gcgccgtgcg
     3901 ccgctggcaa tgcgcctgcg cgcaaacagt acctgggcga tgctggaatt tgtctttaac
     3961 ggcatggtat tcctgctgtt aggtctgcag ctgccgggta ttctggagac gtcgctgatg
     4021 gcggcagaaa tcgaccctaa cgtcgaaatc tggatgctgt ttaccgatat cattctgatt
     4081 tatgcggcgc tgatgctggt ccgtttcggc tggctgtgga cgatgaaaaa gttcagcaac
     4141 cgcttcctga agaagaagcc gatggagttt ggttcgtgga ctacacgaga aatcctgatc
     4201 gcgtctttcg ccggggtgcg tggggcgatc actctggccg gtgtgctctc tatcccgctg
     4261 ctcttgccgg atggtaacgt cttcccggcg cgctatgagc tggtgttcct ggcggctggt
     4321 gtcattctct tctcgctgtt tgtcggcgtg gtgatgttgc ctattctgct acaacacatt
     4381 gaagtcgcgg accattcgca acaattgaaa gaggaacgta ttgcgcgagc ggcaacggca
     4441 gaagtggcga ttgtggcgat ccagaaaatg gaggagcgtc tggcggcgga taccgaagag
     4501 aatatcgata accagctgct cacagaggtt agttctcgcg tcattggtaa cctgcgtcgt
     4561 cgcgccgatg gacgtaatga tgtagaaagt tcaatcctgg aagagaacct ggagcgtcgc
     4621 ttccgtctgg cggcattgcg ttctgaacgt gctgaactgt accacctgcg cgccacgcgg
     4681 gagatcagta acgaaacgct gcaaaaatta ttgcacgatc tcgatttgct tgaagcgttg
     4741 ctgattgaag agaaccaata acgcttggcg gtgtaatcgg taaaaaggtg cagtacatcg
     4801 caccttttta ccgttggtca ggtgaccaga ggctagtgga gattaatgcg cgcggccttg
     4861 ctcaacgcca aagccggtct gggaacggat aaactgcgca cggaacagtt cacgctcacg
     4921 cgcgccttcc gctgagttat cggttgccga gaagaaccag atgccgagga atgccacgct
     4981 gatagagaac agcgccgggt attcatacgg gaagatggct ttttcgtgac cgaggatctg
     5041 tacccaaatc gtcgggccga ggatcatcag caccaccgca gtaatcagtc ctaaccagcc
     5101 gcccagcatc gcgccacggg tagtcagttt cgaccagtac atggaaagca gaatgatcgg
     5161 gaagttacag ctcgctgcga tggcaaacgc cagccccacc ataaaggcga tgttctgatt
     5221 ctcaaacagc acgccgagga taatggcaat cacaccgagg atcagtacgg tgattttcga
     5281 tacccgcagc tcttcacgtt cggtcgcacc ttttttgaag acgttagcgt acaggtcgtg
     5341 agacaccgcc gatgcgcccg ccagcgtcag acccgcaacc accgcaagaa tggtggcgaa
     5401 agcaaccgct gaaataaagc cgaggaacag gttgccaccc actgcattcg ccaggtgaac
     5461 tgctgccatg ttgttaccac caatcagatg gcccgccgcg tctttatatt ccggattcgc
     5521 accaaccagc atgattgcgc cgaagccgat aataaaggtc aggatgtaga agtagcccat
     5581 aaatccggtg gcgtagaaca cgctcttacg tgcttcgcgg gcatcgctga ccgtaaagaa
     5641 gcgcatcaga atatgcggca agccagccgt accaaacatc agtcctagcc ccaaagagag
     5701 cgcggagata ggatctttca ccagcccgcc cggcttcatg atgtcgacac ctttcgggtg
     5761 taccgccatc gcttcgctga acagattgtt gaagctaaag ccgacgtgtt tcatcaccat
     5821 aaaggccata aagctggcac cgaacagcaa cagaacggct ttgataatct gtacccaggt
     5881 ggtcgccagc atgccgccga acaggacgta catcatcatc agcacgccga ccagcaccac
     5941 cgcaatgtga tagttaaggc caaacagcag ctcgatgagt ttacctgcac ctaccatctg
     6001 ggcgataagg taaagcgcca ccaccaccag agaaccacag gccgaaagaa tacgaatcgg
     6061 cccttgtttc agacggtaag aggccacatc ggcaaaggtg tagcgaccga ggttacgcag
     6121 acgttcggca atcaggaaca gaatgattgg ccagcccacc aggaagccca acgagtagat
     6181 cagcccgtca tagccggagg taaacaccag cgcggagatc cccaagaatg aggcggcgga
     6241 catatagtcc ccggcaatcg ccagcccgtt ctgaaaaccg gtgatattgc cgcctgcggt
     6301 gtagtagtca ttacgcgaac gtacgcgttt tgacgcccag taggtaatgc cgagcgtaaa
     6361 cacgacgaaa atcaggaaca taataatcgc ctgccagttt gttggctggc gctctacggc
     6421 cccgctaata gcatccgcgg cgttagctgc gaaagggagt gtggcggcaa gcgccgtcag
     6481 aactcttttc atgatgcttg tacctcatgc aggacttcat tattaagacg gtcgaattcg
     6541 ccgttcgccc gccagatgta gataccagtg agaacaaagg agatcacaat tactccaaca
     6601 ccaatcggaa taccgcgtgt gacgctggtg ttcggattca gcggggtgcc cagccagccg
     6661 ggcgcgaagg cgatcagtaa aataaagccg atataaactg ccagcataat aatcgacagg
     6721 atggtggcaa accgttgccg tttttcgact aactccctga aatgcgcatt gtcttctatc
     6781 cgctgataaa tagtgccatt catcacagat tctccagagg taatgtaggg attgttttaa
     6841 ttcccgcgcc cttatgggag cgggttaacg cccaggtaac cctcgccgaa tgtaggctgg
     6901 ataaggcgtt atatgccgca tccggcaatc aatgcctgat gcgacgctgt cgcgtcttat
     6961 caggcctaca aatcgtttcc gacttgttgc ccggatcgca tccggcaatt gtgggttacg
     7021 atggcatcgc gatagcctgc ttctcttcaa gcagcttctc gactacgcca ggatcggcaa
     7081 gcgtcgaggt atcgcccagg ttgctggtat cgcccgccgc aattttgcgc agaatacggc
     7141 gcataatttt gccggagcgg gttttaggca gggagtcggt ccagtgcagc acgtctggcg
     7201 tcgccagcgg gccaatctct ttacgcaccc agttgcggac ttctgcgtac agttctggtg
     7261 acggttcctc cccgtgatta agcgtgacgt aggcgtagat cgcctgacct ttaatattgt
     7321 gcggaatacc gacgacagcg gcttcggcaa tcttcggatg cgccaccagc gccgactcaa
     7381 tctccgctgt tcccagacgg tgacccgaga cgttcagcac gtcgtccacg cgcccggtga
     7441 tccagtaata gccatcttca tcacgacgcg cgccgtcgcc gctgaaatac atatttttga
     7501 aggtggagaa gtaggtctgt tcaaagcgtt cgtgatcgcc aaacaatgta cgcgcctgac
     7561 caggccagga gtcggtgatc accagactgc cttcggcggc cccctccagc ggatgacctt
     7621 cgttatcgac cagcgccggt tgtacgccga agaacggacg tgttgccgaa ccggctttca
     7681 gctcggtagc gccaggcagt ggggtgatca tgaaaccgcc agtttcggtc tgccaccagg
     7741 tatcgaccac tgggcatttc tcgttgccga tcttcttcca gtaccactcc cacgcttccg
     7801 ggttgattgg ctcgcccacg gaaccgagaa tgcgcagcga cgatcgatcg gtgccttcaa
     7861 tcgctttatc gccttccgcc atcagcgcgc gaatcgccgt gggcgcggta tagagaatat
     7921 tgacctgatg cttgtccacc acctgcgcca tacgggcagg cgtcggccag ttcggtacgc
     7981 cttcaaacat cagcgtggtc gcgccgcagg ccaacgggcc gtacagcaaa taactgtgtc
     8041 cggtaaccca acccacatcg gcggtgcacc agtagatatc gcccggatga taatcaaaga
     8101 catatttaaa ggtcagcgcc gcgtacacca gataaccgcc ggtagtgtgc agtacgcctt
     8161 tcggcttacc ggtagaaccg gaggtataga gaataaacag cggatcttcg gcgttcatct
     8221 cttccgcctg gtgctgatcg ctcgcttgct caaccaggtc gtgccaccac aggtcgcgcc
     8281 cttcctgcca gtcaattttt ccgccagtac gcttcagtac caccacatgc tctacgctgg
     8341 tgacgttcgg gtttttcagc gcgtcatcaa cgtttttctt cagcggaata ctacgcccgg
     8401 cgcgcacgcc ttcgtcggaa gtgatcacca accgtgagtt agaatcgata atgcgcccgg
     8461 caacggcttc cggcgagaag ccgccgaaaa ttaccgaatg caccgcgcca atgcgggcgc
     8521 aggccagcat cgcgaccgcg gcttccggca ccatcggcat ataaatcgcc accacatcac
     8581 cttttttaat gcccagctcg agcagggtat tggcgaagcg gcagacgtcg cggtgcagct
     8641 ctttatagct gatatgtttg ctctggctgg cgtcgtcgcc ttcccagatg atggcggtac
     8701 gatcgccgtt ttcttgcaga tggcggtcaa ggcagtttgc cgccagattc agcgtgccgt
     8761 cctcgtacca tttaatggac acattaccgg gggcaaagga ggtgtttttc accttctggt
     8821 aaggtttgat ccagtcgaga atttttccct gttcgcccca gaaggtatct ggtgcgttaa
     8881 tagattgttg atacatcgcc tcgtactgct gagggtttat caggcaacgg tctgcgatgt
     8941 tggcaggaat ggtgtgtttg tgaatttgac tcatgctttt gttctccttg taggatgtta
     9001 ataatatgtg gcataagcgt taaatgtagg ggtattggca gttttgttta gtatttgggc
     9061 gacagatcac gcaaaagtag aattgtgcaa ataaacggca gggtaatttt tgaaggtcag
     9121 caacaaaagt tgattaattc tttgaggaac atgcagttat gcatgctgtt gaaaaagagg
     9181 aagatactga ctaactctaa agtggtattt tacatgcact tacaattgat taaagacaac
     9241 attcacagtg tggttatttg ttacacatag gggcgagcaa tgtcatgaca gtgtaggtgc
     9301 ggttactgtc gtgaaaaaca ataaaaaccg ccattgcaac aatggcgcaa ttcggatgaa
     9361 gcccctatga caaggataaa aataaacgca cgccgtatct tcagcttatt gattcctttt
     9421 ttctttttca cttctgttca cgctgaacaa acggcagctc ccgcaaaacc tgtaactgtg
     9481 gaagcgaaga atgaaacctt tgccccgcaa catcccgatc aatatctctc ctggaaagcc
     9541 acctcggaac agtcagagcg tgttgacgcc ctggcggaag atccacggct ggtgatcctg
     9601 tgggcggggt atcccttctc gcgcgattac aacaagccgc gtggacatgc ctttgctgtg
     9661 accgatgtgc gtgaaaccct gcgtaccggt gcgccgaaaa acgctgaaga tggtccgcta
     9721 ccgatggcat gctggagttg taaaagcccg gatgtggcgc gtctgatcca gaaagacggc
     9781 gaagatggct acttccacgg aaaatgggcg cgcggcggcc cggaaatcgt caacaactta
     9841 ggttgtgccg attgccataa caccgcctca ccagagttcg ccaaaggcaa accggagtta
     9901 accctttccc gtccgtatgc ggctcgcgcg atggaagcca ttggtaaacc ttttgagaaa
     9961 gccggacgtt tcgaccagca atcgatggtt tgcggtcagt gccatgtgga gtattacttc
    10021 gacggcaaaa acaaagcggt taaattcccg tgggatgacg gcatgaaagt cgaaaatatg
    10081 gagcagtatt acgacaaaat tgccttctct gactggacta actccctgtc gaaaacgcca
    10141 atgctgaaag cgcagcaccc ggaatatgaa acctggacag cgggcattca cggtaaaaac
    10201 aacgtgacct gtatcgactg ccatatgcca aaagtgcaga acgccgaagg caaactctac
    10261 accgaccata aaattggtaa tccgtttgat aacttcgccc agacttgtgc gaactgccat
    10321 acccaggaca aagctgcctt gcaaaaagtg gtcgcggaac gtaagcagtc gattaacgac
    10381 ctgaaaatca aggttgaaga tcaactggtt cacgctcact tcgaagcgaa agcggcgctg
    10441 gatgcaggcg cgacggaagc cgaaatgaag cctattcagg acgatatccg tcatgcccag
    10501 tggcgttggg atctggcgat cgcttcccac ggcattcata tgcacgcacc ggaagaaggt
    10561 ctgaggatgc tcggtacggc gatggataaa gcggcggatg cgcgcactaa actggcgcga
    10621 ctgctggcga ccaaaggcat cacccatgaa atccagatcc cggatatctc gaccaaagag
    10681 aaagcccagc aggccattgg cctgaacatg gaacaaatca aggccgagaa gcaggacttt
    10741 atcaaaacgg tgatcccgca gtgggaagag caggcacgta aaaacggtct gttaagccaa
    10801 taacccccgt tccgcctcgc aaggggcgga aaacacaatg gagtgaatat gagcgtatta
    10861 cgttcgttat taactgccgg ggtgctggcg tcgggcctgt tgtggagcct gaacggaatt
    10921 accgctaccc ctgcggcgca ggcatctggt gatcgctatg aagttaccca gcagcgtaac
    10981 ccggatgccg cctgtctgga ctgtcataaa ccagataccg aaggtatgca tggcaaacat
    11041 gcttccgtta tcaacccgaa taacaaactg ccggtcacct gcaccaactg tcacggccag
    11101 ccatcaccgc aacaccgcga aggggtgaaa gatgtgatgc gctttaacga gccgatgtac
    11161 aaggttggag agcagaacag cgtctgtatg tcctgccatc tgccggaaca gttgcaaaaa
    11221 gcgttctggc cgcacgatgt ccacgtaacc aaagtggcgt gcgccagttg ccattccctg
    11281 catccacagc aagatacgat gcagacgtta agtgacaaag gacggattaa gatttgcgtc
    11341 gattgccaca gcgatcagcg caccaatccg aactttaacc cagcgtctgt tccgttgctt
    11401 aaggagcagc catgacctgg tcacgtcgcc agtttctcac cggcgtcggt gtgctggccg
    11461 ccgtcagcgg caccgcaggg cgcgtggtgg cgaaaacgtt gaatatcaat ggtgtgcgtt
    11521 acggcatggt gcatgacgag tcgttatgca tcggctgtac cgcctgtatg gatgcttgtc
    11581 ggaaagtgaa caaggtgccg gaaggcgtct cgcgcctgac gattattcgt agcgagccgc
    11641 agggcgaatt tcctgatgtg aaatatcgct tcttccgtaa gtcttgccag cactgcgatc
    11701 gtgcgccgtg cgttgacgtc tgcccgaccg gtgcgtcttt tcgcgatgcc gccagcggga
    11761 ttgtcgatgt aaatccggat ctctgcgtcg gttgtcagta ctgcatcgcc gcctgtccgt
    11821 accgcgtgcg ctttatccat ccggtcacga agacagcgga caaatgcgat ttctgccgta
    11881 agaccaattt gcaggccggt aagctgcccg cgtgcgttga ggcttgcccg acgaaggcgc
    11941 tgacgtttgg caatctggac gatcctaaca gtgagatttc gcaactgctg caccagaagc
    12001 ctacttaccg ctacaagctg gcgctgggaa ccaaaccgaa gctgtaccgc gtaccgttta
    12061 aatacgggga ggtgagccaa tgacgcagac ttccgcattt cattttgaat cactggtgtg
    12121 ggactggccg attgccatct acctgttttt gattggtatt tctgccggtc tggtgacgct
    12181 ggccgtgctg ttacgtcgct tctacccgca ggcgggcggt gcagacagta cgttgctgcg
    12241 caccacgctg attgtcggac ctggcgcggt gatcctcggt ctgttgatcc tcgtcttcca
    12301 cctgacaaga ccgtggacct tctggaagct gatgttccac tacagtttta cctcggtgat
    12361 gtcgatgggg gtgatgctgt ttcagcttta catggtggtg ctggtgctgt ggctggcgaa
    12421 aatctttgaa catgatttgc ttgccctgca acaacgctgg ttgccgaagc tggggatcgt
    12481 gcaaaaggtt ctgagcctgc tgacgcccgt tcatcgcgga ctggaaacat tgatgctggt
    12541 gctggcggtg ctgctggggg cttataccgg ctttctgcta tcggcgctga aatcgtatcc
    12601 gttcctcaat aacccgatcc tgccggtgct gttcctcttc tccggcatct cgtccggtgc
    12661 ggcggtggcg ctgatcgcca tggcgatacg ccaacgcagt aacccgcatt ccacggaagc
    12721 gcactttgta caccgtatgg agatccctgt ggtatggggc gaaatcttcc tgctggtggc
    12781 gttttttgtc ggtctggcgc tgggcgatga cggtaaagtg cgtgcgctgg tggcggcatt
    12841 gggcggcggt ttctggacgt ggtggttctg gcttggtgtc gccgggctgg ggttgattgt
    12901 gccaatgttg ctcaaaccgt gggtgaatcg tagttccggc attcctgccg tgctggcggc
    12961 gtgtggggcc agcctggtcg gcgtgttgat gctgcgcttt ttcattctct acgccgggca
    13021 gttaacggtg gcgtaagcca gaacagaggt ggtttctgga cgtattcctt cctgaagtcg
    13081 gttttctggc gttgttgtta agtctcgggg tcaacgtgtt gaccccgttg acggcctttg
    13141 cgggagtgcg gttgcgctgg cctgccatga tgcgactcac ttgcatcggc attctggcgc
    13201 agttcgcgct cctgctgctc gcctttggcg tactgacgta ttgttttctc atcagcgatt
    13261 tctcggtcat ttatgtcgcc caacatagct acagcctgct gtcgtgggaa ctcaaactgg
    13321 cggcggtgtg gggcggtcat gaaggttcgc tgctgctttg ggtgctgctg ctttccgcct
    13381 ggagcgcgct gtttgcctgg cattatcggc agcaaaccga tccgctattt ccgctgacgc
    13441 tagccgtttt atctctcatg ctcgccgcac tgctactgtt tgtagtgctg tggtccgatc
    13501 ccttcgtgcg gatatttccg ccagcaatcg aaggccgcga tctcaatccg atgctgcaac
    13561 atcccggtct tatctttcat ccaccgctgc tttatctcgg ctacggcggt ttgatggtgg
    13621 cggcgagcgt ggcgctggcg agtttactgc gcggcgagtt tgatggtacc tgcgcccgaa
    13681 tttgctggcg ctgggcacta ccaggctggg gggcattaac ggcggggatc atcctcggtt
    13741 cctggtgggc ctattgcgaa ctgggctggg gcggctggtg gttctgggat ccggtggaaa
    13801 acgcctcttt attaccctgg ctttctgcca ctgcgctgct gcacagtttg tccctgacac
    13861 gccagcgggg gattttccgc cactggtcgc tgttactggc gatagttact ctgatgctgt
    13921 cgctgctggg caccttaatt gtccgttctg gcattctggt ttcggttcat gcgttcgcgc
    13981 tggataacgt tcgcgccgtg ccgttgttca gcctgtttgc actgattagc cttgcgtctc
    14041 tgactctgta tggctggcga gcgcgggacg gtggcccggc ggtgcgtttt tcggggttat
    14101 cgcgggaaat gttaatcctc gctacgctgt tgctgttttg cgcagtgcta ctgatcgtgc
    14161 tggtgggaac gctttatccg atgatttacg gcctgctggg ctggggacgc ctctccgttg
    14221 gcgcgccgta ttttaaccgt gcgacgttac cgtttggtct gttgatgctg gtggtgattg
    14281 tgctggcgac gtttgtctct ggcaaacgcg tgcagcttcc ggcgctgctg gcgcatgcgg
    14341 gcgtgctgtt atttgccgca gggatcgtgg tctccagcgt cagccgtcag gagatcagcc
    14401 tgaatttaca gccgggtcag caggtgacgc tggcaggata cgccttccgt tttgagcgtc
    14461 tcgatctgca agccagaggc aattacacca gcgaaaaagc gatagtggca ctgtttgacc
    14521 atcagcaacg tattggtgaa ctgacgccgg agcggcgttt ttatgaagca cgccgtcagc
    14581 aaatgatgga accgtcaatt cgctggaacg gcatccatga ctggtatgcg gtcatggggg
    14641 agaaaactgg gccggatcgt tacgcttttc gtttgtatgt acaaagcggt gtgcgctgga
    14701 tctggggggg aggattgttg atgattgcgg gcgcattgtt aag
//
LOCUS       DAMZFT010000123        14716 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.123, whole genome shotgun sequence.
ACCESSION   DAMZFT010000123 DAMZFT010000000
VERSION     DAMZFT010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14716)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14716)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14716
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.123"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..329
                     /gene="grxD"
                     /locus_tag="PN977_003873"
     CDS             <1..329
                     /gene="grxD"
                     /locus_tag="PN977_003873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="monothiol glutaredoxin 4"
                     /protein_id="HDI6043866.1"
                     /translation="IQRQIAENPILLYMKGSPKLPSCGFSAQAVQALAACGERFAYVD
                     ILQNPDIRAELPKYANWPTFPQLWVDGELVGGCDIVIEMYQRGELQQLIKETAAKYKS
                     EEPDAE"
     gene            complement(380..4996)
                     /locus_tag="PN977_003874"
     CDS             complement(380..4996)
                     /locus_tag="PN977_003874"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001361457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HDI6043867.1"
                     /translation="MADNLNPSSPLPDVFSPATRDWFLRAFKQPTAVQSQTWHVAARG
                     EHALVIAPTGSGKTLAAFLYALDQLFREGGEDTREAHKRKTSRILYISPIKALGTDVQ
                     RNLQIPLQGIADERRRRGETEVNLRVGIRTGDTPAQERSKLTRNPPDILITTPESLYL
                     MLTSRARETLRGVETVIIDEVHAVAGSKRGAHLALSLERLDALLHTSAQRIGLSATVR
                     SASDVAAFLGGDRPVTVVNPPTMRHPQIRIVVPVANMDDVSSVASGTGEDSHAGREGS
                     IWPYIETGILDEVLRHRSTIVFTNSRGLAEKLTARLNELYAARLQRSPSIAVDAAHFE
                     STSGATSNRVQSSDIFIARSHHGSVSKEQRAITEQALKSGELRCVVATSSLELGIDMG
                     AVDLVIQVATPLSVASGLQRIGRAGHQVGGVSKGLFFPRTRRDLVDSAVIVECMFAGR
                     LENLTPPHNPLDVLAQQTVAAAAMDALQVDEWYSRVRRAAPWKDLPGRVFDATLDMLS
                     GRYPSGDFSAFRPKLVWNRETGILTARPGAQLLAVTSGGTIPDRGMYSVLLPEGEEQA
                     GSRRVGELDEEMVYESRVNDIITLGATSWRIQQITRDQVIVTPAPGRSARLPFWRGEG
                     NGRPAELGEMIGDFLHLLADGAFFSGTIPPWLAEENTIANIQGLIDEQRNATGIVPGS
                     RHLVLERCRDEIGDWRIILHSPYGRRVHEPWALAIAGRIHALWGADASVVASDDGIVA
                     RIPDTDGKLPDAAIFLFEPEKLLQIVREAVGSSALFAARFRECAARALLMPGRTPGHR
                     TPLWQQRLRASQLLEIAQGYPDFPVILETLRECLQDVYDLPALERLMRRLNGGEIQIS
                     DVTTTTPSPFAASLLFGYVAEFMYQSDAPLAERRASVLSLDSELLRNLLGQVEPGELL
                     DPQVIRQVEEELQRLAPGRRAKGEEGLFDLLRELGPMTVEDLAQRHTGSSEEIASYLE
                     NLLAVKRIFPTMISGQERLACMDDAARLRDALGVRLPESLPEIYLHRVSYPLRDLFLR
                     YLRAHALVTAEQLAHEFSLGIAIVEEQLQQLREQGLVMNLQQDIWVSDEVFRRLRLRS
                     LQAAREATRPVAATTYARLLLERQGVLPATDGSPALFASTSPGVYEGVDGVMRVIEQL
                     AGVGLPASLWESQILPARVRDYSPEMLDELLATGAVIWSGQKKLGEDDGLVALHLQEY
                     AAESFTPAEADQANRSALQQAIIAVLADGGAWFAQQISQRICDKIGESVDPSALQEAL
                     WALVWQGVITSDIWAPLRALTRSSSNARTSTRRSHRARRGRPVYTQPVSPLVSYNTPK
                     LAGRWSLLQVEPLNDTERMLALAENMLDRYGIISRQAVIAENIPGGFPSMQTLCRSME
                     DSGRIMRGRFVEGLGGAQFAERLTIDRLRDLATQTAQTRHYTPVALSANDPANVWGNL
                     LPWPAHPATLVPTRRAGALVVVSGGKLLLYLAQGGKKMLVWQEKEELLAPEVFHALTT
                     ALRREPRRRFTLTEVNDLPVRQTPMFTLLREAGFSSSPQGLDWE"
     gene            complement(5089..5736)
                     /gene="rnt"
                     /locus_tag="PN977_003875"
     CDS             complement(5089..5736)
                     /gene="rnt"
                     /locus_tag="PN977_003875"
                     /EC_number="3.1.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease T"
                     /protein_id="HDI6043868.1"
                     /translation="MSDNAQLTGLCDRFRGFYPVVIDVETAGFNAKTDALLEIAAITL
                     KMDEQGWLMPDTTLHFHVEPFVGANLQPEALAFNGIDPNDPDRGAVSEYEALHEIFKV
                     VRKGIKASGCNRAIMVAHNANFDHSFMMAAAERASLKRNPFHPFATFDTAALAGLALG
                     QTVLSKACQTAGMDFDSTQAHSALYDTERTAVLFCEIVNRWKRLGGWPLPAAEEV"
     gene            complement(5839..6246)
                     /gene="gloA"
                     /locus_tag="PN977_003876"
     CDS             complement(5839..6246)
                     /gene="gloA"
                     /locus_tag="PN977_003876"
                     /EC_number="4.4.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HDI6043869.1"
                     /translation="MRLLHTMLRVGDLQRSIDFYTKVLGMKLLRTSENPEYKYSLAFV
                     GYGPETEEAVIELTYNWGVDKYELGTAYGHIALSVDNAAEACEKIRQNGGNVTREAGP
                     VKGGTTVIAFVEDPDGYKIELIEEKDAGRGLGN"
     gene            complement(6327..7424)
                     /gene="nemA"
                     /locus_tag="PN977_003877"
     CDS             complement(6327..7424)
                     /gene="nemA"
                     /locus_tag="PN977_003877"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-ethylmaleimide reductase"
                     /protein_id="HDI6043870.1"
                     /translation="MSSEKLYSPLKVGAITAANRIFMAPLTRLRSIEPGDIPTPLMAE
                     YYRQRASAGLIISEATQISAQAKGYAGAPGIHSPEQIAAWKKITAGVHAENGHMAVQL
                     WHTGRISHASLQPGGQAPVAPSALSAGTRTSLRDENGQAIRVETSMPRALELEEIPGI
                     VNDFRQAISNAREAGFDLVELHSAHGYLLHQFLSPSSNHRTDQYGGSVENRARLVLEV
                     VDAGIEEWGADRIGIRVSPIGTFQNTDNGPNEEADALYLIEQLGKRGIAYLHMSEPDW
                     AGGEPYTDAFREKVRARFHGPIIGAGAYTVEKAETLIGKGLIDAVAFGRDWIANPDLV
                     ARLQRKAELNPQRAESFYGGGAEGYTDYPTL"
     gene            complement(7461..8060)
                     /gene="nemR"
                     /locus_tag="PN977_003878"
     CDS             complement(7461..8060)
                     /gene="nemR"
                     /locus_tag="PN977_003878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310384.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NemR"
                     /protein_id="HDI6043871.1"
                     /translation="MNKHTEHDTREHLLATGEQLCLQRGFTGMGLSELLKTAEVPKGS
                     FYHYFRSKEAFGVAMLERHYAAYHQRLTELLQSGEGNYRDRILAYYQQTLNQFCQHGT
                     ISGCLTVKLSAEVCDLSEDMRSAMDKGARGVIALLSQALENGRDSHCLTFCGEPLQQA
                     QVLYALWLGANLQAKISRNYEPLENALAHVKTIIATPAV"
     gene            8163..8402
                     /locus_tag="PN977_003879"
     CDS             8163..8402
                     /locus_tag="PN977_003879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1289 domain-containing protein"
                     /protein_id="HDI6043872.1"
                     /translation="MAEQLEFFPVQSPCRGICQSDERGFCRGCFRSRDERFNWNKMSD
                     GEKQEVLRLCRQRLMRKLRANKPASSDEPEQPSLF"
     gene            8451..9347
                     /locus_tag="PN977_003880"
     CDS             8451..9347
                     /locus_tag="PN977_003880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HDI6043873.1"
                     /translation="MVQRITIAPQGPEFSRFVMGYWRLMDWNMSARQLVSFIEEHLDL
                     GVTTVDHADIYGGYQCEAAFGEALKLAPHLRERMEIVSKCGIATTAREENVIGHYITD
                     RDHIIKSAEQSLINLATDHLDLLLIHRPDPLMDADEVADAFKHLHQSGKVRHFGVSNF
                     TPAQFALLQSRLPFTLATNQVEISPVHQPLLLDGTLDQLQQLRVRPMAWSCLGGGRLF
                     NDDYFQPLRDELAVVAEELNAGSIEQVVYAWVLRLPSQPLPIIGSGKIERVRAAVEAE
                     TLKMTRQQWFRIRKAALGYDVP"
     gene            9428..9949
                     /gene="sodC"
                     /locus_tag="PN977_003881"
     CDS             9428..9949
                     /gene="sodC"
                     /locus_tag="PN977_003881"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Cu-Zn] SodC2"
                     /protein_id="HDI6043874.1"
                     /translation="MKRFSLAILALVVATGAQAASEKVEMNLVTSQGVGQSIGSVTIT
                     ETDKGLEFSPDLKALPPGEHGFHIHAKGSCQPATKDGKASAAESAGGHLDPQNTGKHE
                     GPEGAGHLGDLPALVVNNDGKATDAVIAPRLKSLDEVKDKALMVHVGGDNMSDQPKPL
                     GGGGERYACGVIK"
     gene            complement(9950..11962)
                     /locus_tag="PN977_003882"
     CDS             complement(9950..11962)
                     /locus_tag="PN977_003882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HDI6043875.1"
                     /translation="MNASSWSLRNLPWFRATLAQWRYALRNTIAMCLALTVAYYLNLD
                     EPYWAMTSAAVVSFPTVGGVISKSLGRIAGSLLGAIAALLLAGHTLNEPWFFLLSMAA
                     WLGFCTWACAHFTNNVAYAFQLAGYTAAIIAFPMVNITEASQLWDIAQARVCEVIVGI
                     LCGGMMMMILPSNSDATALLTALKNMHARLLEHASLLWQPETTDAIRAAHEGVIGQIL
                     TMNLLRIQAFWSHYRFRQQNARLNALLHQQLRMTSVISSLRRMLLNWPSPPGATREIL
                     EQLLTALASSQTDVYTVARIIAPLRPTNVADYRHVAFWQRLRYFCRLYLQSSQELHRL
                     QSGVDDHTRLPRTSGLARHTDNAEAMWSGLRTFCTLMMIGTWSIASQWDAGANALTLA
                     AISCVLYSAVAAPFKSLSLLMRTLVLLSLFSFVVKFGLMVQISDLWQFLLFLFPLLAT
                     MQLLKLQMPKFAALWGQLIVFMGSFIAVTNPPVYDFADFLNDNLAKIVGVALAWLAFA
                     ILRPGSDARKSRRHIRALRRDFVDQLSRHPSLSESEFESLTYHHVSQLSNSQDAMARR
                     WLLRWGVVLLNCSHVVWQLRDWESRSDPLSRVRDNCISLLRGVMSERGVQQKSLAATL
                     EELQRICDSLARHHQPAARELAAIVWRLYCSLSQLEQAPPQGTLAS"
     gene            complement(11962..12819)
                     /locus_tag="PN977_003883"
     CDS             complement(11962..12819)
                     /locus_tag="PN977_003883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416161.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HDI6043876.1"
                     /translation="MSIKTIKYFSTIIVAVVAVLAGWWLWNYYMQSPWTRDGKIRAEQ
                     VSITPQVSGRIVELNIKDNQLVNAGDLLLTIDKTPFKIAELNAQAQLAKAQSDLAKAN
                     NEANRRRHLSQNFISAEELDTANLNVKAMQASVDAAQATLKQAQWQLAQTEIRAPVSG
                     WVTNLTTRIGDYADTGKPLFALVDSHSFYVIGYFEETKLRHIREGAPAQITLYSDNKT
                     LQGHVSSIGRAIYDQSVESDSSLIPDVKPNVPWVRLAQRVPVRFALDKVPGDVTLVSG
                     TTCSIAVGQ"
     gene            complement(12822..13058)
                     /locus_tag="PN977_003884"
     CDS             complement(12822..13058)
                     /locus_tag="PN977_003884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1656 domain-containing protein"
                     /protein_id="HDI6043877.1"
                     /translation="MKFMLNATGLPLQDLVFGASVYFPPFFKAFAFGFVIWLVVHRLL
                     RGWIYAGDIWHPLLMDLSLFAICVCLALAILIAW"
     gene            13259..13693
                     /gene="slyA"
                     /locus_tag="PN977_003885"
     CDS             13259..13693
                     /gene="slyA"
                     /locus_tag="PN977_003885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416159.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SlyA"
                     /protein_id="HDI6043878.1"
                     /translation="MESPLGSDLARLVRIWRALIDHRLKPLELTQTHWVTLHNIHQLP
                     PDQSQIQLAKAIGIEQPSLVRTLDQLEEKGLISRQTCASDRRAKRIKLTEKAEPLISE
                     MEAVINKTRAEILHGISAEELEQLIKLIAKLEHNIIELQAKG"
     gene            complement(13740..14207)
                     /gene="slyB"
                     /locus_tag="PN977_003886"
     CDS             complement(13740..14207)
                     /gene="slyB"
                     /locus_tag="PN977_003886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein SlyB"
                     /protein_id="HDI6043879.1"
                     /translation="MIKRVLVVSMVGLSLVGCVNNDTLSGDVYTASEAKQVQNVSYGT
                     IVNVRPVQIQGGDDSNVIGAIGGAVLGGFLGNTVGGGTGRSLATAAGAVAGGVAGQGV
                     QSAMNKTQGVELEIRKDDGNTIMVVQKQGNTRFSPGQRVVLASNGSQVTVSPR"
     gene            14481..>14716
                     /locus_tag="PN977_003887"
     CDS             14481..>14716
                     /locus_tag="PN977_003887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HDI6043880.1"
                     /translation="MKSGRFIGVMSGTSLDGVDVVLATIDEHRVAQLASLSWPIPVSL
                     KQAVLDICQGQQLTLSQFGQLDTQLGRLFADAVNA"
BASE COUNT         3587 a         3884 c         3896 g         3349 t
ORIGIN      
        1 aaatccaacg ccagattgct gaaaacccga tcctgctgta catgaaaggt tcaccgaaac
       61 tgccgagctg cggtttctct gcccaggcag tccaggcgct tgccgcatgt ggcgaacgtt
      121 ttgcctatgt tgatatcctg cagaatccgg acattcgtgc ggaactgccg aaatatgcta
      181 actggccgac cttcccgcaa ctgtgggttg acggcgagct ggtcggcggt tgtgatatcg
      241 tgatcgaaat gtatcagcgt ggcgaactgc agcagttgat caaagaaact gccgctaaat
      301 acaagtctga agagccggac gctgaataat ccgtcctgca atactgacgg atgcgaacgg
      361 gcatccgtca gttctttatc tattcccaat ccagcccttg tggcgaactt gaaaatcccg
      421 cctcacgcag cagcgtaaac atcggcgttt gtcggactgg tagatcattc acttctgtca
      481 gcgtaaagcg ccgccgtggt tcgcgacgca gtgcggtagt cagcgcgtgg aaaacctctg
      541 gggcgagtaa ttcctctttt tcctgccaga ccagcatttt tttgccacct tgcgccagat
      601 aaagtaacaa tttgccgcca gaaacgacca ccagcgcacc cgcccgacgc gttggaacca
      661 gcgttgccgg atgtgcaggc cagggcagaa gatttcccca cacattagcc ggatcgttgg
      721 cggagagcgc cactggtgta tagtggcgcg tttgcgcggt ttgtgtcgcc aggtcacgca
      781 atcggtcaat agtcagacgt tcagcgaatt gcgcgccacc cagaccttct acaaaacgac
      841 cccgcataat tcgcccggag tcttccatac ttcgacaaag cgtttgcatc gatggaaaac
      901 cgccagggat attttcggct atcaccgcct gacgactgat gatgccgtag cggtcgagca
      961 tattttccgc cagcgccagc atcctttcgg tatcgtttag tggctccacc tgcaataacg
     1021 accagcgtcc ggccagcttt ggtgtgttgt aagataccag cggcgagacg ggttgcgtat
     1081 agacaggacg cccacgacga gcccggtgac tgcggcgagt cgaggtgcgt gcgttggaac
     1141 tgctacgggt gagggcgcgt aacggtgccc aaatgtcgct ggtaatgacg ccttgccaga
     1201 ccagcgccca taacgcctct tgcagagccg agggatcaac cgattcgccg attttatcgc
     1261 atatccgttg gctgatttgt tgtgcaaacc aggctcctcc gtcagccaga acagcgatta
     1321 tcgcttgctg cagcgccgaa cgattcgcct gatctgcttc ggcgggagtg aacgattctg
     1381 cggcatattc ctgtagatgc agtgccacca ggccgtcatc ttcacccagc tttttttgtc
     1441 ccgaccagat aaccgctccg gttgccagta attcatcgag catttctggc gaatagtcgc
     1501 gtacgcgggc aggcaggatc tggctttccc agagtgaggc gggtaaaccg actcctgcaa
     1561 gctgttcgat cacccgcatc acgccatcca cgccctcata aacgcctggc gatgttgagg
     1621 caaagagtgc cgggctacca tcggtggcgg gtaatacgcc ctgacgttcc agcagcaatc
     1681 gcgcataggt cgtggccgca acgggacgtg tcgcttctct ggcggcttgc agcgagcgca
     1741 aacgcagacg acgaaatact tcatcgctca cccagatgtc ttgttgcaga ttcatcacca
     1801 gaccctgttc gcgcagttgc tgaagttgct cttcgacaat ggcaataccg agactaaact
     1861 catgagccag ttgttcagcc gtgaccagag catgagcccg gagatagcgc agaaagaggt
     1921 cgcgaagcgg gtaactgact cggtgcaaat aaatctctgg cagtgactct ggtagtcgta
     1981 cgccgagggc atcgcgcagc ctggcggcat catccataca ggcaagacgc tcctgcccgc
     2041 taatcatcgt cgggaagatt cgttttactg caagaagatt ttccagatac gacgcaatct
     2101 cttcactgct gcctgtatgc cgttgcgcca ggtcttcaac ggtcattggc cccagttcgc
     2161 gcagcaggtc gaacaatcct tcttcacctt tcgctcttct gccaggtgcc agtcgttgca
     2221 actcttcttc cacctggcga atgacctgcg ggtcgagtaa ttcccccggt tcgacctgtc
     2281 cgagtagatt gcgcagtaac tcgctgtcca gcgacagtac ggatgcccgg cgctctgcca
     2341 gcggggcgtc gctctggtac ataaattccg cgacatagcc gaacaataaa ctggcggcga
     2401 aaggcgaggg cgtagtggtc gtcacatcgg atatttgaat ttcgccaccg ttcaggcgac
     2461 gcatcaaacg ttccagcgcg ggaagatcat aaacatcttg cagacattcg cgtagggttt
     2521 cgagaatgac cggaaaatcc ggatatccct gagcgatttc cagcaactga ctggcgcgca
     2581 gtcgttgttg ccagagcggg gtgcgatggc ccggcgtgcg gcccggcatt aataatgccc
     2641 gcgcggcgca ttcgcgaaaa cgggcggcga aaagtgccga gctgcctacc gcctcgcgga
     2701 caatttgcag caacttttct ggttcaaaca aaaaaatcgc ggcatcaggc aatttaccat
     2761 cggtgtcagg aatgcgcgca acaatgccgt catcactggc aaccacagac gcgtcagcgc
     2821 cccatagtgc atgtattcgc ccggcaatcg ccagcgccca gggttcatgc acccgtcttc
     2881 catagggaga gtgcaaaata atacgccagt caccaatttc atcacggcac cgttcgagga
     2941 caagatgacg actacctgga acgatacccg tcgcgttgcg ctgctcgtca atcaacccct
     3001 gaatattggc gatcgtattt tcttctgcca accacggggg aatagtcccg gaaaagaacg
     3061 cgccatccgc cagtaaatgg agaaaatcgc cgatcatctc gcctaattct gccggacgtc
     3121 cgttaccttc accacgccag aagggaaggc gggcagaacg acctggagca ggagtcacaa
     3181 tcacctgatc gcgggtgatt tgctggatcc gccatgaggt tgcgccaaga gtgataatat
     3241 cgttcacccg tgactcatat accatctcct catccagttc acccacccgc cgcgaaccgg
     3301 cctgttcttc accttcgggt aataacacgc tatacatgcc acgatccgga atggtgccgc
     3361 cgctggtaac cgccaacaat tgagcgccag gtcgggcggt caatatcccg gtctccctgt
     3421 tccagaccag tttggggcga aaagcagaaa aatcgccaga gggatagcgc ccggaaagca
     3481 tatccagcgt ggcgtcaaaa acacgtcctg gcagatcttt ccacggtgcg gcacggcgta
     3541 cgcgggagta ccattcgtct acctgtaatg catccatcgc cgcggcggca acggtttgct
     3601 gcgcaaggac gtcgagtgga ttatgcggtg gtgtcaggtt ttccagcctg cctgcgaaca
     3661 tacactctac aatgactgcg gaatcgacta agtcacgccg ggttcggggg aaaaaaagcc
     3721 ctttagatac accgccaacc tgatgtccgg cacgtccaat gcgttgtaac ccactggcaa
     3781 cagaaagcgg cgttgccacc tgaatcacca gatccaccgc gcccatatca atccccagtt
     3841 cgagactgga ggttgcgacc acgcagcgta attcacccga tttcagcgcc tgttcggtga
     3901 ttgctcgttg ttctttagag acggagccgt ggtgtgagcg ggcaataaaa atgtcgctac
     3961 tttgtacacg gttagaggtt gcgccggagg tcgactcgaa atgggccgca tcaacggcga
     4021 tagacgggga acgctgtaag cgagcggcgt aaagctcatt taatcgtgcc gtcagttttt
     4081 ccgccagccc gcgtgaatta gtaaagacaa tggtcgagcg atggcgcaaa acttcatcaa
     4141 ggatacccgt ttcaatatat ggccagatgg agccttctcg gccggcatgg ctgtcttcgc
     4201 cggtgccgct ggcgaccgat gagacatcat ccatattggc gaccggtacg acaatccgta
     4261 tctgcggatg gcgcattgtg ggcgggttga ctaccgtaac cgggcgatcg ccaccaagaa
     4321 atgctgccac atcgctggct gagcgtacag tggcagaaag gccaattcgc tgtgctgagg
     4381 tgtggagcag cgcatcgagc cgctccagac ttaacgccag atgcgcacca cgtttactgc
     4441 ccgctaccgc gtggacttca tcaataatta ccgtttcgac gccgcgtagc gtttcgcgcg
     4501 cgcgggaggt cagcatcaga tagagtgatt cgggtgtggt gatcagaata tccggcggat
     4561 tacgggtgag tttgctgcgt tcctgtgcag gcgtatcgcc agtacggatc cccacgcgaa
     4621 gattgacttc cgtttcgccg cgccgccgcc gttcatcggc aataccctgt aacgggatct
     4681 gcaagttgcg ctgaacgtcg gtgcccagag ctttgatcgg tgaaatatag agaatgcgtg
     4741 aggttttacg cttatgcgcc tcgcgggtat cttcgccgcc ttcgcggaag agctgatcga
     4801 gggcgtagag aaatgctgcc agcgttttcc cggagccagt cggtgcaatc accagcgcat
     4861 gttcgcctcg cgccgccaca tgccaggttt gcgactggac agcggtcggc tgtttaaagg
     4921 cgcgaagaaa ccagtcgcgg gtcgccggtg aaaacacgtc cggcaggggc gatgaagggt
     4981 ttagattatc tgccattaac gctattcccc gtgaatatca tgcatgatgc gtggaatagc
     5041 ctcactatag atgcctgaat cattgcatgt catcaggcat cgactcgatt acacctcttc
     5101 ggcggcaggt agcggccagc ctcccagacg tttccagcgg ttgacgattt cacaaaacag
     5161 cacagcagtg cgttcggtgt cgtacagcgc ggagtgcgcc tgggtgctgt cgaagtccat
     5221 gccagcggtc tggcaagcct ttgacaatac ggtttgtccg agtgccagcc cggccagtgc
     5281 agcagtgtca aaagtggcga aagggtggaa cgggttacgt ttcagtgagg cgcgttctgc
     5341 ggcggccatc ataaagctgt gatcgaaatt ggcgttgtgc gccaccataa tggcgcggtt
     5401 acagccgctc gctttaatgc ctttacgtac aactttaaaa atttcgtgca gcgcctcgta
     5461 ttcgctgacc gcgccgcgat cgggatcgtt cgggtcaata ccgttgaagg cgagggcctc
     5521 tggctgcaaa tttgcgccga caaatggttc gacgtggaaa tgtaatgtgg tgtccggcat
     5581 cagccagcct tgttcatcca ttttcagggt gatggcggca atctcaagca gcgcatcggt
     5641 tttggcatta aatccggctg tttcaacatc gatcacaaca ggataaaaac cacgaaaacg
     5701 gtcgcacaga ccggtaagtt gagcgttatc ggacatctga ttctcttaat acggataaat
     5761 tgcagcgcgc attatgacaa atattacttg tcgatgcagt aaagatgcgg gcgtgatggg
     5821 ttcacgcccg gcaggagatt agttgcccag accgcgaccg gcgtctttct cttcgattaa
     5881 ctcaattttg taaccgtccg gatcttccac aaacgcgata accgtagtac cgccttttac
     5941 cggacccgct tcacgggtca cgttaccccc gttttgacgg attttttcgc acgcttcagc
     6001 ggcgttatca acgctaagcg cgatgtgacc ataagcagtg ccgagttcgt atttatccac
     6061 gccccagttg taggtcagtt caatcaccgc ttcttcggtt tccgggccgt agccaacaaa
     6121 cgccagtgag tatttgtatt ccgggttttc gctggtacgc agcagtttca tgcccagtac
     6181 tttggtataa aaatcgatgg agcgttgcag atcgccaacg cgcagcatgg tatgaagaag
     6241 acgcatcttt atatcctcaa aatggctaac agattcaaaa tgttgtttta gtatagcggc
     6301 ggctttgcgc cgctcgcaat gttggattac agcgtcgggt aatccgtgta gccttccgcg
     6361 ccgccaccgt aaaaactttc ggcacgctgt gggttaagct cagctttgcg ttgcaagcgg
     6421 gcgaccagat ccgggttcgc aatccagtca cgaccaaatg ccaccgcatc aattaaccct
     6481 ttgccgatca gcgtttcggc tttttctacc gtgtatgcac ctgcgccgat aatcggaccg
     6541 tggaaacggg cgcgtacttt ttcgcggaac gcatcagtat acggctcacc ccccgcccaa
     6601 tctggttctg acatatgcag ataagcaatg ccgcgtttac ccagttgttc aatcagatac
     6661 agtgcatcgg cttcttcatt cgggccgtta tctgtgttct ggaaagtacc gattggcgaa
     6721 acgcgaatgc caatgcgatc ggcaccccat tcttcaatcc ctgcatcgac cacttccagc
     6781 accagacgtg cgcgattttc cacgctgccg ccgtactgat cggtacgatg gtttgaagaa
     6841 ggagaaagga actgatgcag caaataacca tgagcagagt ggagctctac cagatcaaaa
     6901 ccggcttcac gcgcgttaga aatggcctga cggaaatcat tgacgatacc cggaatctct
     6961 tccagttcaa gcgcacgcgg catggatgtt tcaacacgga tcgcctgacc attttcatcg
     7021 cgcagagaag tacgtgttcc cgcgctaagt gcggaaggcg ctaccggtgc ctgaccgcca
     7081 ggttgcaggc tggcgtgaga aatgcgtccg gtgtgccaca gctgtacggc catatgacca
     7141 ttttcagcat gaacgccagc ggtaattttt ttccatgcgg caatttgttc cggactatgg
     7201 atgccaggtg caccagcata cccttttgcc tgggcagaaa tttgcgtggc ttcgctaata
     7261 atcaaaccgg cactggcacg ttggcgatag tattccgcca tcaacggggt agggatgtca
     7321 cccggttcaa tactgcgaag acgcgtcagc ggtgccataa aaatacggtt tgccgccgtg
     7381 atcgcgccca ctttcagtgg ggaatacagt ttttcagatg acataaaggc tcccggtcgt
     7441 ctggtgataa aaaatgcctg ctaaacggca ggcgtcgcaa taatggtttt tacatgtgcc
     7501 agcgcgtttt ccagtggctc gtaattgcgc gaaattttgg cctgcagatt cgcacccagc
     7561 cacagtgcgt aaagcacttg tgcctgttgc agcggttcgc cacaaaaggt taaacaatgg
     7621 ctatcacggc cattttccag cgcctgcgag agcagggcga tcacgccgcg agcgccttta
     7681 tccatcgcgc tacgcatatc ttctgacaga tcgcacactt cggcagagag ttttactgtc
     7741 aggcaaccac tgatggttcc atgttggcaa aactggttca gtgtttgctg gtaataagcc
     7801 agtatgcggt cgcggtagtt accttcgccg gattgcagca actcagtcag tcgctggtga
     7861 tatgcggcgt aatggcgctc aagcatggca acgccaaacg cttctttaga gcgaaagtag
     7921 tgatagaagg accctttcgg cacttcagca gtttttagga gttcgcttag ccccatcccg
     7981 gtgaatccac gttgcaggca aagttgctcg cccgtcgcca ggagatgttc gcgagtatca
     8041 tgttcggtgt gtttgttcat gcgttggagt gtagtagacc agtcggtcta gtgcaagcaa
     8101 agttgctact ttgggcatta acagcttcaa tagtggtaat aatcttatct gaggagggcg
     8161 aagtggcgga gcaattagag ttctttcccg tccagagccc gtgccgggga atttgccagt
     8221 ctgacgaacg cggattttgt cgcggctgct ttcgcagtcg ggatgagcgt tttaactgga
     8281 ataaaatgag cgatggcgaa aaacaagagg tactgcgcct ttgtcggcag cgattaatgc
     8341 gcaaattacg tgcaaataag ccggcgtcat cagatgaacc ggagcaacca tcactctttt
     8401 gagcctaatc ttgcgtatac tcctctcata tctgataaga ggaagcgatt atggttcagc
     8461 gtattactat tgcgccgcaa ggcccggagt tttcccgttt tgtgatgggc tactggcgat
     8521 tgatggactg gaatatgtcc gcccgccagc tggtcagttt tattgaagag catctggatc
     8581 tcggcgtgac caccgtggac catgctgata tttatggtgg ctatcagtgc gaagcggcgt
     8641 ttggcgaggc gctgaaactg gcacctcacc tgcgtgaacg gatggaaatc gtcagtaaat
     8701 gcggtatcgc gacgaccgcg cgtgaagaaa acgtcattgg tcattacatc actgaccgcg
     8761 atcacatcat taagagcgcc gaacagtcgc taattaatct cgcgaccgat catctggatc
     8821 tgctgttaat ccaccgacca gacccgttaa tggatgccga tgaagtggcg gacgcgttta
     8881 aacatctgca tcagagcggc aaagtgcgcc attttggcgt atccaacttc acgcccgcgc
     8941 aatttgccct gttgcaatca cgcctgccgt ttacccttgc cactaatcag gtggaaatat
     9001 ccccggtgca tcagccgtta ctgctggatg gcacgctcga ccaactgcaa caactgcgtg
     9061 ttcgtccgat ggcgtggtcc tgccttggtg gtggtcgtct gtttaatgat gattatttcc
     9121 agccgctgcg tgatgaactg gctgtggtgg cggaggagtt aaacgcgggc tcgattgaac
     9181 aggtggttta cgcctgggta ttacgtttac cgtcgcagcc gctgccaatt atcggctcag
     9241 gtaaaattga gcgcgtacgg gcagctgtcg aagctgaaac actgaaaatg acccgtcaac
     9301 aatggttccg tatccgtaaa gcggcattgg gttacgacgt accgtaagca cttttaggaa
     9361 tagccgccgt tcaaaaatgt gtcactggtt tacacttatt caggaatgca caatgaacgg
     9421 aggtcctatg aaacgtttta gtctggccat tctggcgctg gttgttgcga ccggcgcaca
     9481 agctgccagt gaaaaagtcg agatgaacct cgtcacgtcg caaggggttg ggcagtcaat
     9541 tggtagcgtc accattactg aaaccgataa aggtctggag ttttcgcccg acctgaaagc
     9601 attacccccc ggtgaacatg gcttccatat tcatgccaaa ggaagctgcc agccagccac
     9661 caaagatggc aaagccagcg ccgcggaatc cgcaggcggg catcttgatc cacaaaatac
     9721 cggtaaacat gaagggccag aaggtgccgg gcatttaggc gatctgcctg cactggtcgt
     9781 caataatgac ggcaaagcta ccgatgccgt catcgcgcct cgtctgaaat cactggatga
     9841 agtcaaagac aaagcactga tggtccacgt tggcggcgat aatatgtccg atcaacctaa
     9901 accgctgggc ggtggcggtg aacgctatgc ctgtggtgta attaagtaat taagaggcca
     9961 gcgtaccttg cggcggtgct tgctcaagtt gcgaaagcga gcagtacagc cgccagacaa
    10021 ttgccgccag ctcacgggcg gcaggttgat gatgacgggc aaggctgtcg caaattcgct
    10081 gtaattcttc aagtgtggcc gccagtgatt tttgctgaac gccacgctca ctcatcactc
    10141 cgcgcaacag tgaaatacag ttatcccgta ctcgcgataa cggatcggaa cgcgattccc
    10201 agtcgcgcaa ttgccagaca acatgagaac agttcagcag cactacaccc cagcgtaata
    10261 accaacggcg agccatcgca tcctggctgt tactcaactg actgacgtga tgataagtga
    10321 gcgattcaaa ttcgctttca ctcagtgatg gatggcggct tagttgatcg acaaaatccc
    10381 ggcgcagcgc gcgaatatga cggcgacttt tacgagcatc cgatcctgga cgcagaatgg
    10441 cgaacgctaa ccacgccagc gcaacgccaa cgatttttgc cagattatcg ttaagaaaat
    10501 cagcaaaatc atacaccggg ggattagtga cagcgataaa cgaacccata aaaacaatca
    10561 gttgccccca cagtgcggca aatttgggca tctgcaattt aagaagctgc atcgtcgcca
    10621 gcagtggaaa gagaaacagt aaaaattgcc acagatcgct aatctggacc atcaggccaa
    10681 atttgaccac aaagctgaat agcgaaagta acaccagcgt acgcatcagc agtgacaacg
    10741 acttaaacgg agctgcgacg gcggagtaga gtacgcagct aatcgctgcc agcgttaatg
    10801 cattggcacc ggcatcccat tgcgaagcaa tactccatgt gccaatcatc atcaacgtac
    10861 aaaatgtacg cagcccgctc cacatagctt cggcgttatc ggtatgacga gccaggccgg
    10921 atgtccgtgg gagtctggta tgatcatcta caccgctttg cagacgatgt aattcctgac
    10981 tactttgcag ataaagtcgg caaaaataac gtagtcgctg ccagaaggcg acgtgccgat
    11041 agtcggcgac gttggtcggg cgtagcgggg cgataatacg tgcgacggtg taaacatctg
    11101 tttgcgaact ggcgagcgcc gtcagcagct gttcgagaat ttctcgtgtg gcacctggcg
    11161 gtgagggcca gttgagcaac atacgtcgca ggctggagat gacactggtc atacgtaatt
    11221 gctggtggag cagcgcatta aggcgtgcgt tttgctggcg aaaacgatag tggctccaga
    11281 aagcctggat acggagcaaa ttcatggtca gtatctgccc aatcacccct tcatgtgctg
    11341 cacgaatggc atcggttgtt tcaggctgcc agagtaaact ggcatgttcc agcaatcggg
    11401 cgtgcatgtt tttcaatgcg gttaaaaggg ctgtagcatc ggaattgctc ggcaggatca
    11461 tcatcatcat gccgccgcac aaaataccga caatcacctc gcaaacgcgc gcctgagcga
    11521 tatcccacag ctggctggcc tcagtaatat taaccatcgg aaaggcgatg atggcagccg
    11581 tgtagcccgc cagttgaaat gcatacgcga cattattcgt gaagtgcgca caggcccagg
    11641 tacaaaagcc aagccacgcc gccatgctca aaagaaaaaa ccacggctca ttaagtgtat
    11701 gtccggcaag aagcagtgcc gcaatggctc cgagcaaact gccagcgatg cgtccgaggc
    11761 ttttgctgat gacaccgccc acggtgggaa agctaaccac ggcagccgag gtcatcgccc
    11821 aatagggttc atccagattt aaataatagg caaccgtcag cgccagacac atggcaatgg
    11881 tattgcgtaa cgcataacgc cattgcgcca gcgtggccct gaaccagggc aaattgcgca
    11941 aggaccatga cgatgcgttc attattgacc tacggcaatg ctacaggtgg tgcccgacac
    12001 cagcgtgaca tcgcctggga ctttatccag cgcaaagcgg acgggaacgc gttgggcgag
    12061 tcgaacccag gggacgttag gtttcacatc cggaataagg cttgagtcac tttcaacgct
    12121 ctgatcataa atcgcccgac cgatactgga aacgtgaccc tgtaacgttt tgttatcact
    12181 atagagggta atttgtgctg gtgcaccttc gcggatatgg cgcaacttgg tttcttcaaa
    12241 ataaccaatg acgtaaaacg agtggctgtc gacaagggca aacagcggtt tccctgtgtc
    12301 agcgtaatcg ccgatgcggg tagtgaggtt agtcacccat ccactcaccg gggcgcgaat
    12361 ttccgtttgc gccagttgcc attgtgcctg tttcagcgtc gcctgtgcgg catcaacact
    12421 ggcctgcatc gctttaacat tgaggttagc ggtatcgagt tcttcggcag aaataaaatt
    12481 ttgtgagaga tgacgacgac gattcgcttc gttattggct tttgcgaggt cagattgcgc
    12541 tttcgccagc tgagcctgtg cgttcagctc ggcgatctta aagggcgttt tatcgatcgt
    12601 gagcagaaga tcccccgcgt taaccagctg gttgtctttt atattcagct caacaatacg
    12661 tcccgacacc tgaggggtga tagaaacctg ttctgcgcgt attttcccgt cgcgtgtcca
    12721 cggagattgc atgtaataat tccacagcca ccatccggca agaaccgcaa ctaccgctac
    12781 aatgattgtt gagaaatact taattgtttt aattgacata gttaccacgc aatcagtatt
    12841 gccagggcaa ggcaaacgca aatcgcaaac agcgataaat ccattaacaa gggatgccag
    12901 atgtcaccag cgtagatcca gccacgaagc aggcggtgta cgacaagcca gatgacgaat
    12961 ccaaacgcga aggctttgaa aaacggagga aagtagacgg acgcaccgaa caccagatct
    13021 tgtaagggca atcctgttgc attgagcata aacttcacaa agtagattcc tttacgaccg
    13081 aatatattgc gttagattaa taaatattct ttaagtgcga aaaatttacg cgcaatttct
    13141 gaaaaatatg tctgacggta accaaatgca gcaatacatt tgttttagca atacaattgc
    13201 tgcacactat tctaaaagcc gcataatatc ttagcaagct aattataagg agatgaaatt
    13261 ggaatcgcca ctaggttctg atctggcacg gttggtgcgc atatggcgtg ctctgataga
    13321 ccatcgcctg aaaccgctgg agttaacaca aacccattgg gttacgttac acaatatcca
    13381 tcagttacct ccagaccagt cgcaaattca actggcaaaa gcgattggca tcgagcagcc
    13441 atcactggtc cgtactctgg accaactgga agaaaaaggg ttaatttcgc gtcaaacttg
    13501 tgccagcgat cgtcgggcta aacgtattaa actgacggaa aaggcagagc cactgatcag
    13561 cgaaatggaa gctgttatta acaaaacccg cgcggaaatt ttacatggca tctccgcaga
    13621 ggaactggaa caactgatta agctcatcgc aaaacttgag cataatatca ttgagttaca
    13681 ggccaaaggg tgaaatgaag ggggcgtgtg gtcagtaacc tgaccacacg caaacttatt
    13741 tagcgcgggg aaacggtcac ctgactgcca ttgctggcca gtacgacacg ttggcccgga
    13801 gagaaacgag tgttgccttg tttctgtacc accatgatgg tattaccatc gtctttacga
    13861 atttccagct cgacaccctg cgttttgttc attgcactct gtacgccctg accggctacg
    13921 ccacctgcaa cagcgcctgc tgcagtagcc agagaacgcc cggttccgcc gccaacagta
    13981 tttcccagga aaccaccaag aacagcaccg ccaattgcgc cgataacgtt ggaatcatca
    14041 ccgccctgaa tctgtaccgg acgtacgtta acgatggtgc catagctgac attctgtact
    14101 tgtttcgctt cagaagcggt ataaacatcc cctgacaggg tgtcgttatt aacacaaccg
    14161 acaagagaca gacctaccat tgaaacaacc aatacgcgtt taatcattga aaaatctcct
    14221 gttcaccatg aaacgccacg caagcatccc tcatggtcaa agtatatggc atatgtgaat
    14281 ccatggttac atgttctacc aaatatgagc aaatcataat gaaagtagga tcaaccaatt
    14341 ataaacaaaa cattcatgat gattattgat ttgaagataa tgttttacta ccaacgataa
    14401 aaatcggtca ttccgcatgg tattcacgaa aagtttatgt ttgagtggtg cgcaaacgtc
    14461 agattgaaaa aggatgaact atgaaatcgg gtcgctttat tggcgttatg tcaggcacca
    14521 gccttgatgg tgttgatgtt gtgttggcga caattgatga acaccgggtc gcacagctgg
    14581 caagtttgag ctggccgatc ccggtgtctc tgaaacaggc tgtactggat atttgccagg
    14641 gccagcagct tacactttcg cagtttggac agcttgatac tcaactcggg cgactttttg
    14701 ctgatgcggt caatgc
//
LOCUS       DAMZFT010000124        14614 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.124, whole genome shotgun sequence.
ACCESSION   DAMZFT010000124 DAMZFT010000000
VERSION     DAMZFT010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14614)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14614)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14614
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.124"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(<1..748)
                     /gene="pdxK"
                     /locus_tag="PN977_003888"
                     /gene_synonym="thiJ"
     CDS             complement(<1..748)
                     /gene="pdxK"
                     /locus_tag="PN977_003888"
                     /gene_synonym="thiJ"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine/pyridoxal/pyridoxamine kinase"
                     /protein_id="HDI6043881.1"
                     /translation="MSSLLLFNDKSRALQADIVAVQSQVVYGSVGNSIAVPAIKQNGL
                     NVFAVPTVLLSNTPHYDTFYGGAIPDEWFSGYLRALQERDALRQLRAVTTGYMGTASQ
                     IKILAEWLTALRKDHSDLLIMVDPVIGDIDSGIYVKPDLPEAYRQYLLPLAQGITPNI
                     FELEILTGKNCRDLDSAIAAAKSLLSDTLKWVVITSASGNEENQEMQVVVVSADSVNV
                     ISHSRVKTDLKGTGDLFCAQLISGLLKGEAL"
     gene            853..1221
                     /locus_tag="PN977_003889"
     CDS             853..1221
                     /locus_tag="PN977_003889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeK family protein"
                     /protein_id="HDI6043882.1"
                     /translation="MKKIICLVVTLLMTLPAYAKLTAHEEARINAMLEGLAQKKDLIF
                     VRNGDEHTCDEAVSHLRLKLGNTRNRIDTAEQFIDKVASSSSITGKPYIVKMPGKSDE
                     NAQPFLHALIAQTDKTVPAQ"
     gene            complement(1224..2135)
                     /gene="cysM"
                     /locus_tag="PN977_003890"
     CDS             complement(1224..2135)
                     /gene="cysM"
                     /locus_tag="PN977_003890"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase B"
                     /protein_id="HDI6043883.1"
                     /translation="MSTLEQTIGNTPLVKLQRMGPNNGSEVWLKLEGNNPAGSVKDRA
                     ALSMIVEAEKRGEIKPGDVLIEATSGNTGIALAMIAALKGYRMKLLMPDNMSQERRAA
                     MRAYGAELILVTKEQGMEGARDLALEMANRGEGKLLDQFNNPDNPFAHYTTTGPEIWQ
                     QTGGRITHFVSSMGTTGTITGVSRFMREQSKPVTIVGLQPEEGSSIPGIRRWPAEYLP
                     GIFNASLVDEVLDIHQRDAENTMRELAVREGIFCGVSSGGAVAGALRVAKANPGAVVV
                     AIICDRGDRYLSTGVFGEEHFSQGAGI"
     gene            complement(2269..3366)
                     /gene="cysA"
                     /locus_tag="PN977_003891"
     CDS             complement(2269..3366)
                     /gene="cysA"
                     /locus_tag="PN977_003891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter ATP-binding
                     protein CysA"
                     /protein_id="HDI6043884.1"
                     /translation="MSIEIANIKKSFGRTQVLNDISLDIPSGQMVALLGPSGSGKTTL
                     LRIIAGLEHQTSGHIRFHGTDVSRLHARDRKVGFVFQHYALFRHMTVFDNIAFGLTVL
                     PRRERPNAAAIKAKVTKLLEMVQLAHLADRYPAQLSGGQKQRVALARALAVEPQILLL
                     DEPFGALDAQVRKELRRWLRQLHEELKFTSVFVTHDQEEATEVADRVVVMSQGNIEQA
                     DAPDQVWREPATRFVLEFMGEVNRLQGTIRGGQFHVGAHRWPLGYTPAYQGPVDLFLR
                     PWEVDISRRTSLDSPLPVQVLEASPKGHYTQLVVQPLGWYNEALTVVMHGDDAPQRGE
                     RLFVGLQHARLYNGDERIETRDEELALAQSA"
     gene            complement(3356..4231)
                     /gene="cysW"
                     /locus_tag="PN977_003892"
     CDS             complement(3356..4231)
                     /gene="cysW"
                     /locus_tag="PN977_003892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysW"
                     /protein_id="HDI6043885.1"
                     /translation="MAEVTQLKRYDARPINWGKWFLIGIGMLVSAFILLVPMIYIFVQ
                     AFSKGLMPVLQNLADPDMLHAIWLTVMIALIAVPVNLVFGILLAWLVTRFNFPGRQLL
                     LTLLDIPFAVSPVVAGLVYLLFYGSNGPLGGWLDEHNLQIMFSWPGMVLVTIFVTCPF
                     VVRELVPVMLSQGSQEDETAILLGASGWQMFRRVTLPNIRWALLYGVVLTNARAIGEF
                     GAVSVVSGSIRGETLSLPLQIELLEQDYNTVGSFTAAALLTLMAIITLFLKSMLQWRL
                     ENQEKRAQQEEHHEH"
     gene            complement(4231..5064)
                     /gene="cysT"
                     /locus_tag="PN977_003893"
     CDS             complement(4231..5064)
                     /gene="cysT"
                     /locus_tag="PN977_003893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysT"
                     /protein_id="HDI6043886.1"
                     /translation="MFAVSSRRVLPGFTLSLGTSLLFVCLILLLPLSALVMQLSEMSW
                     AQYWEVITNPQVVAAYKVTLLSAFVASIFNGVFGLLMAWILTRYRFPGRTLLDALMDL
                     PFALPTAVAGLTLASLFSVNGFYGEWLAKFDIKVTYTWLGIAVAMAFTSIPFVVRTVQ
                     PVLEELGPEYEEAAETLGATRWQSFCKVVLPELSPALVAGVALSFTRSLGEFGAVIFI
                     AGNIAWKTEVTSLMIFVRLQEFDYPAASAIASVILAASLLLLFSINTLQSRFGRRVVG
                     H"
     gene            complement(5064..6080)
                     /gene="cysP"
                     /locus_tag="PN977_003894"
     CDS             complement(5064..6080)
                     /gene="cysP"
                     /locus_tag="PN977_003894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate/sulfate ABC transporter
                     substrate-binding protein CysP"
                     /protein_id="HDI6043887.1"
                     /translation="MAVNLLKKNSLALVASLLLAGHVQATELLNSSYDVSRELFAALN
                     PPFEQQWAKDNGGDKLTIKQSHAGSSKQALAILQGLKADVVTYNQVTDVQILHDKGKL
                     IPADWQSRLPNNSSPFYSTMGFLVRKGNPKNIHDWNDLVRSDVKLIFPNPKTSGNARY
                     TYLAAWGAADKADGGDKAKTEQFMTQFLKNVEVFDTGGRGATTTFAERGLGDVLISFE
                     SEVNNIRKQYEAQGFEVVIPKTNILAEFPVAWVDKNVQANGTEKAAKAYLNWLYSPQA
                     QTIITDYYYRVNNPEVMDKLKDKFPQTELFRVEDKFGSWPEVMKTHFTSGGELDKLLA
                     AGRN"
     gene            complement(6238..7029)
                     /gene="ucpA"
                     /locus_tag="PN977_003895"
     CDS             complement(6238..7029)
                     /gene="ucpA"
                     /locus_tag="PN977_003895"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase UcpA"
                     /protein_id="HDI6043888.1"
                     /translation="MGKLTGKTALITGALQGIGEGIARTFARHGANLILLDISPEIEK
                     LADELCGRGHRCTAVVADVRDPASVAAAIKRAKEKEGRIDILVNNAGVCRLGSFLDMS
                     DEDRDFHIDINIKGVWNVTKAVLPEMIARKDGRIVMMSSVTGDMVADPGETAYALTKA
                     AIVGLTKSLAVEYAQSGIRVNAICPGYVRTPMAESIARQSNPEDPESVLTEMAKAIPM
                     RRLADPLEVGELAAFLASDESSYLTGTQNVIDGGSTLPETVSVGI"
     gene            complement(7158..8015)
                     /gene="murR"
                     /locus_tag="PN977_003896"
     CDS             complement(7158..8015)
                     /gene="murR"
                     /locus_tag="PN977_003896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MurR"
                     /protein_id="HDI6043889.1"
                     /translation="MLYLTKIRNAESEFTGNEQKIADFLRANVSELKSVSSRKMAKQL
                     GISQSSIVKFAQKLGAQGFTELRMALIGEYSASREKTNATALHLHSSITSDDSLEVIA
                     RKLNREKEQALEQTCALFDYARLQKIIEVISKAPFIQITGLGGSALVGRDLSFKLMKI
                     GYRVACEADTHVQATVSQALKKGDVQIAISYSGSKKEIVLCAEAARKQGATVIAITSL
                     TDSPLRRLAHYTLDTVSGETEWRSSSMSTRTAQNSVTDLLFVGLVQLNDVESLKMIER
                     SSELTQRLK"
     gene            8179..9075
                     /gene="murQ"
                     /locus_tag="PN977_003897"
     CDS             8179..9075
                     /gene="murQ"
                     /locus_tag="PN977_003897"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HDI6043890.1"
                     /translation="MQLEKMITEGSNAASAEIDRVSTLEMCRIINDEDKTVPLAVERV
                     LPDIAAAIDVIHAQVSGGGRLIYLGAGTSGRLGILDASECPPTYGVKPGLVVGLIAGG
                     EYAIQHAVEGAEDSREGGVNDLKNIGLTAQDVVVGIAASGRTPYVIAGLEYAHQLGCR
                     TVGISCNPGSAVSTTAEFAITPVVGAEVVTGSSRMKAGTAQKLVLNMLSTGLMIKSGK
                     VFGNLMVDVVATNEKLHVRQVNIVKNATGCSAEQAETALIACKRNCKTAIVMVLKNLD
                     ADEAKKCLDQHGGFIRKALEKE"
     gene            9079..10503
                     /gene="murP"
                     /locus_tag="PN977_003898"
     CDS             9079..10503
                     /gene="murP"
                     /locus_tag="PN977_003898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylmuramic acid transporter subunit
                     IIBC"
                     /protein_id="HDI6043891.1"
                     /translation="MAKEISSELLNTILTRVGGPGNIASCGNCMTRLRLGVHDSSLVD
                     PNIKTLEGVKGVILTSDQVQVVFGPGKAHRAAKAMSELLGEAPIQDAAEIAAQNKRQL
                     KAKQTSGVQQFLAKFATIFTPLIPGFIAAGLLLGIATLIATVMHVPADAQGTLPDALN
                     FMKVFSKGLFTFLVILVGYNAAQAFGGTGVNGAIIAALFLLGYNPAATTGYYAGFHDF
                     FGLPIDPRGNIIGVLIAAWACARIEGMVRRFMPDDLDMLLTSLITLLITATLAYLIIM
                     PLGGWLFEGMSWLFMHLNSNPLGCAVLAGLFLIAVVFGVHQGFIPVYLALMDSQGFNS
                     LFPILSMAGAGQVGAALALYWRAQPHSALRSQVRGAIIPGLLGVGEPLIYGVTLPRMK
                     PFVTACLGGAAGGLFIGLIAWWGLPMGLNSAFGPSGLVALPLMTSAQGILPAMAVYAG
                     GILVAWVCGFIFTTLFGCRNVNLD"
     gene            10508..11809
                     /gene="pbp4b"
                     /locus_tag="PN977_003899"
     CDS             10508..11809
                     /gene="pbp4b"
                     /locus_tag="PN977_003899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin binding protein PBP4B"
                     /protein_id="HDI6043892.1"
                     /translation="MKRTMLYLSLLAVSCSVSAAKYPVLTESSPEKAGFNVERLNQMD
                     RWISQQVDAGYPGVNLLIIKDNQIVYRKAWGAAKKYDGSALMAQPVKATTGTLYDLAS
                     NTKMYATNFALQKLMSEGKLHPDDRIAKYIPGFADSPNDAIKGKNTLRISDLLHHSGG
                     FPADPQYPNKAVAGALYSQDKGQTLEMIKRTPLEYQPGSKHIYSDVDYMLLGFIVESV
                     TGQPLDHYVEESIYRPLGLTHTVFNPLLKGFKPQQIAATELNGNTRDGVIHFPNIRTS
                     TLWGQVHDEKAFYSMGGVSGHAGLFSNTGDIAVLMQTMLNGGGYGDVQLFNAETVKMF
                     TTSSKEDATFGLGWRVNGNATMTPTFGTLASPQTYGHTGWTGTVTVIDPVNHMAIVML
                     SNKPHSPVADPQKNPNMFESGQLPIATYGWVVDQVYAALKQ"
     gene            complement(11867..12766)
                     /gene="yfeX"
                     /locus_tag="PN977_003900"
     CDS             complement(11867..12766)
                     /gene="yfeX"
                     /locus_tag="PN977_003900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311329.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphyrinogen peroxidase"
                     /protein_id="HDI6043893.1"
                     /translation="MSQVQSGILPEHCRAAIWIEANVKGEVDALRAASKTFADKLATF
                     EAKFPDAHLGAVVAFGNNTWRALSGGVGAEELKDFPGYGKGLAPTTQFDVLIHILSLR
                     HDVNFSVAQAAMEAFGDCIEVKEEIHGFRWVEERDLSGFVDGTENPAGEETRREVAVI
                     KDGVDAGGSYVFVQRWEHNLKQLNRMSVHDQEMMIGRTKEANEEIDGDERPETSHLTR
                     VDLKEDGKGLKIVRQSLPYGTASGTHGLYFCAYCARLHNIEQQLLSMFGDTDGKRDAM
                     LRFTKPVTGGYYFAPSLDKLMAL"
     gene            complement(12862..13437)
                     /locus_tag="PN977_003901"
     CDS             complement(12862..13437)
                     /locus_tag="PN977_003901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpoE-regulated lipoprotein"
                     /protein_id="HDI6043894.1"
                     /translation="MKSLRLMLCAMPLMLTGCSTMSSVNWSAANPWNWFGSSTKVSEQ
                     GVGELTASTPLQEQAIADALDGDYRLRSGMKTANGNVVRFFEVMKGDNVAMVINGDQG
                     TISRIDVLDSDIPADTGVKIGTPFSDLYSKAFGNCQKADSDDNRAVECKAEGSQHISY
                     QFSGEWSGPEGLMPSDDTLKNWKVSKIIWRR"
     gene            complement(13498..13947)
                     /locus_tag="PN977_003902"
     CDS             complement(13498..13947)
                     /locus_tag="PN977_003902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2919 domain-containing protein"
                     /protein_id="HDI6043895.1"
                     /translation="MKSTEFHPVHYDAHGRLRLPLLFWLVLLLQARTWVLFVIAGASR
                     EQGTALLNLFYPDHDNFWLGLIPGIPAVLAFLLSGRRASFPRIWHVLYFLLLLAQVVL
                     LCWQPWLWLNGESVSGIGLALVVADIVALIWLLTNRRLRACFNEEKE"
     gene            complement(13934..14359)
                     /locus_tag="PN977_003903"
     CDS             complement(13934..14359)
                     /locus_tag="PN977_003903"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311332.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family acetyltransferase"
                     /protein_id="HDI6043896.1"
                     /translation="MEIRVFRQEDFEEVITLWERCDLLRPWNDPEMDIERKMNHDVSL
                     FLVAEVNGEVVGTVMGGYDGHRGSAYYLGVHPEFRGRGIANALLNRLEKKLIARGCPK
                     IQINVPEDNDMVLGMYERLGYEHADVLSLGKRLIEDEEY"
BASE COUNT         3464 a         4103 c         3675 g         3372 t
ORIGIN      
        1 ttaacgcctc ccctttcagc aaaccactga taagctgagc acaaaacagg tcgccagtcc
       61 ctttcaggtc agttttaacc cgtgaatggg aaatgacatt cacgctgtcg gcactgacca
      121 ccaccacctg catctcctga ttttcttcat taccggaggc gctggtaatc accacccatt
      181 ttaatgtgtc tgaaagcaga ctttttgcgg cagcaatggc actgtcgaga tcgcggcaat
      241 ttttaccggt caggatttcc agctcaaaga tattgggggt aattccctgc gccagcggca
      301 gtaaatattg tcgatacgct tcgggaaggt caggtttgac ataaattccg ctatcaatat
      361 cgccgatcac cggatcgacc ataatcaata agtcagaatg gtctttgcgt agcgcagtca
      421 gccactcggc aaggattttg atttgcgatg ccgttcccat atagcccgtg gttacagcac
      481 gaagttggcg cagcgcatca cgctcctgaa gcgcacgcaa ataaccgcta aaccattcat
      541 ccggaatcgc accaccgtag aaagtgtcat aatgcggcgt attgctcagc aataccgtcg
      601 gcacggcaaa gacattcagg ccgttctgtt tgatagcagg caccgcaatg ctgttgccta
      661 cgctgccgta cactacctgc gactgcacgg caacgatatc cgcctgcagt gccctgctct
      721 tatcgttaaa caacaacaaa ctactcattt aattttttct ccttgccgat gatcctcatc
      781 gtattccaac cgaaacttta cctgattctg gcagtcaaat cggctattac aaaacaagga
      841 taaggtaatt cagtgaagaa aatcatttgt ctggtcgtta cgctgctaat gacactcccc
      901 gcttatgcga agttaacggc ccatgaagaa gcccgtatca acgctatgct ggagggatta
      961 gcacagaaaa aggatttaat atttgtgcgt aacggtgatg aacatacctg cgatgaagcg
     1021 gtttctcatc tgcgactgaa gctgggcaat acccgtaatc gcattgatac agccgagcag
     1081 tttattgaca aggtggcctc gtcgtcatcg attaccggga agccgtatat cgtgaagatg
     1141 cccggtaaga gcgatgagaa cgcacagcct tttttacatg cgttaattgc gcagacggat
     1201 aaaacggtgc ctgcgcaata atcttaaatc cccgccccct ggctaaaatg ctcttcccca
     1261 aacaccccgg tagaaaggta acgatcgccg cgatcgcaga tgatcgccac aaccaccgcg
     1321 ccggggttag cttttgccac ccgcagtgct ccggcaaccg cgccgccgga gctgacgcca
     1381 cagaatattc cttcccgcac tgccagttcg cgcatggtgt tttccgcatc gcgctgatga
     1441 atatccagca cctcatccac cagcgaagcg ttgaaaatcc ccggcagata ttccgcaggc
     1501 cagcggcgaa tgcccggaat actgctgcct tcttccggct gcaggccaac aatggtcacc
     1561 ggtttggatt gttcgcgcat aaagcgtgaa acgccggtga tagtgccggt tgttcccatg
     1621 ctggagacaa aatgggtaat gcgcccgcca gtctgctgcc agatttccgg cccggtggtg
     1681 gtgtaatggg cgaagggatt atcaggatta ttgaactgat cgagcagctt tccttcacca
     1741 cgattggcca tctccagcgc cagatcgcgc gcaccttcca tgccctgctc tttggtgacc
     1801 agaatcagtt ccgcaccgta ggcacgcatt gccgcacggc gttcctggct catgttgtcg
     1861 ggcatcagca atttcatgcg atagccttta agcgcggcaa tcattgccag cgcaatgccg
     1921 gtgttaccac tggtggcttc gatcaacaca tcacccggtt taatctcgcc gcgcttttcc
     1981 gcctcgacga tcatcgaaag tgccgcacga tcttttaccg aacctgccgg gttattgcct
     2041 tccagtttca accagacttc actgccgtta ttcggcccca ttcgctgcaa cttcaccaga
     2101 ggcgtattgc caattgtttg ttctaatgta ctcacgatct ctgtccatac gtggtgttgc
     2161 ctgatgcgac gcttgcgcgt cttatcagac ctacaggtta caaaccttgc cattaaaaag
     2221 cccggatcgc gggaagcgcc tccgggcgtt taacattcac tcaacctatc aggcgctttg
     2281 tgcgagagca agttcctcat cgcgggtttc aatacgctcg tcgccgttat acagccgcgc
     2341 atgttgcaga ccaacgaata aacgctcgcc acgttgcggg gcgtcgtcgc cgtgcatcac
     2401 gaccgtcagc gcttcgttgt accaccccag cggctgcacg actaattggg tgtagtggcc
     2461 tttcgggctg gcttccagta cctgtaccgg cagcggcgaa tcgaggctgg tacggcggct
     2521 gatatccact tcccacggac gcaggaagag atccactggc ccctgatacg caggtgtata
     2581 gcccagcggc cagcgatgtg cgccaacatg gaactgcccg ccgcgaatgg ttccctgcag
     2641 gcggttaact tcgcccataa attcgagtac aaaacgggtc gctggttcgc gccagacctg
     2701 gtccggtgca tcagcctgct cgatattgcc ctggctcatc accactacac gatcggctac
     2761 ttcggtcgct tcttcctggt catgggtcac aaacacgctg gtgaatttca gctcttcatg
     2821 taactgacgc agccagcgac gcaactcttt acgcacctgc gcatccagcg cgccaaacgg
     2881 ttcatcaagc agcagaattt gcggttctac agccagcgcg cgcgccagcg ccacacgctg
     2941 tttctggccg ccggaaagct gcgcaggata gcggtctgcc agatgggcaa gctgcaccat
     3001 ctccagcaat tttgtcactt tcgctttgat cgccgccgca ttcgggcgct cgcgacgcgg
     3061 tagcaccgtc aggccaaaag cgatattgtc gaataccgtc atatggcgga acaacgcgta
     3121 atgctggaac acgaaaccga ctttacggtc gcgcgcatgc aggcggctaa cgtcggtgcc
     3181 gtggaagcga atatgcccgc tggtttgatg ctccagcccg gcgataatcc gcagcagcgt
     3241 ggttttcccg gaaccggacg gccccagcaa cgcgaccatc tgaccagaag gaatatccag
     3301 tgagatatcg ttcagcacct gggtgcgacc aaaagacttc ttaatattgg caatctcaat
     3361 gctcatgatg ttcctcctgc tgcgcgcgtt tttcctgatt ctccagacgc cactgcaaca
     3421 tactctttaa aaacagggtg ataatcgcca tcagcgtcaa caacgcggca gcggtaaagg
     3481 agccgacggt gttgtagtcc tgctccagca attcaatctg taacggcagc gaaagtgttt
     3541 cgccgcgaat cgagccggaa actaccgaca ccgcaccaaa ctcgccaatc gcgcgggcgt
     3601 tggtcagcac cacgccataa agcagcgccc agcggatgtt cggcagcgtg acgcgacgga
     3661 acatctgcca gccagatgcg cccagcaaaa tcgctgtttc gtcttcctgg ctgccctggc
     3721 ttaacattac cggcaccagc tcgcgcacca caaacggaca ggtcacgaag atggtgacca
     3781 gcaccattcc cggccaggag aacataattt gcagattatg ttcatccagc cagccaccga
     3841 gcgggccgtt agagccgtaa aacagcagat acaccagacc ggcaaccacc ggcgacacgg
     3901 caaacggaat gtccagcagc gtcagcagca actggcgtcc ggggaagtta aagcgcgtca
     3961 ccagccaggc cagcagaatg ccgaacacca ggttcaccgg cacggcaatc agcgcaatca
     4021 tcaccgtcag ccagatggcg tgcagcatgt ccggatcggc cagattctgt aaaaccggca
     4081 tcagcccttt gctgaacgcc tgcacgaaga tgtaaatcat cggcaccagc aggatgaacg
     4141 ccgaaaccag catcccgatg ccaatcagaa accatttgcc ccagttaatc gggcgcgcgt
     4201 cataacgctt caattgtgtc acttccgcca ttaatgacct accacacgcc gaccaaagcg
     4261 actttgcaga gtgttaattg agaacagcag caacagggat gccgcgagga tcaccgaagc
     4321 aatcgcgctc gccgccgggt aatcaaactc ctgtaagcga acaaaaatca tcagcgacgt
     4381 cacttccgtc ttccaggcaa tgttcccggc gatgaaaatc accgcgccaa attcaccaag
     4441 actacgggta aacgacagcg ccacgcccgc caccagcgcc ggagaaagtt ccggcagcac
     4501 cactttgcag aaactctgcc agcgcgttgc gccaagcgtt tccgccgctt cttcatattc
     4561 cgggcctaac tcttccagca ccggctgcac ggtacgcacc acaaacggaa tgctggtaaa
     4621 ggccatcgcc accgcgatcc ccagccaggt ataggtgact ttgatatcaa acttcgccag
     4681 ccattcaccg taaaaaccgt tcacggaaaa gagcgaggcc agcgttaaac cggcgactgc
     4741 cgttggcagc gcaaagggta aatccatcag cgcatcaagc agcgtgcggc ccggaaagcg
     4801 atagcgggtt aagatccacg ccatcagcag accgaaaacg ccgttaaaaa tcgacgcgac
     4861 aaacgccgac agcagcgtca ctttataggc tgcgaccacc tgcgggttgg tgatcacctc
     4921 ccagtactgc gcccagctca tctcagagag ctgcatcacc agcgcggaga gcggcagcag
     4981 caaaatcagg caaacaaaca gcagactggt gccgaggctt aaggtaaagc ccggcagcac
     5041 gcgtcttgat gaaactgcaa acatcagtta cgccccgccg ctaacagctt gtctaactcg
     5101 ccgccgctgg tgaagtgggt tttcatcact tccggccagg agccaaattt gtcttccacg
     5161 cggaacagtt cggtctgcgg gaatttgtct ttcagtttgt ccatcacctc cgggttattt
     5221 acgcggtagt aatagtcggt gatgatggtt tgcgcctgcg ggctgtagag ccagttcagg
     5281 taagcttttg ctgctttttc cgtaccgttg gcctgcacgt ttttatcgac ccacgccacc
     5341 gggaattccg ccagaatatt ggttttcgga atcaccactt caaagccctg cgcttcatac
     5401 tgtttgcgga tgttgttcac ttccgactcg aagctgatca gcacatcgcc caggccgcgc
     5461 tcggcaaaag tggtggtcgc gccacgaccg ccagtatcga acacttcaac gtttttcagg
     5521 aactgggtca taaactgttc ggttttggct ttgtcgccac cgtcagcttt atccgctgcg
     5581 ccccatgccg ccagataggt ataacgcgcg ttaccggagg tttttgggtt cgggaaaatc
     5641 agcttcacgt cggagcgcac cagatcgttc cagtcgtgga tattcttcgg gttgccctta
     5701 cgtaccagga aacccatggt ggagtagaac ggcgagctat tattcggcag gcgcgactgc
     5761 cagtcggccg ggatcagctt gcctttatcg tgcaggattt gtacgtcggt cacctggtta
     5821 taagtgacaa cgtcggcttt taagccctgt aaaatcgcca gcgcctgttt tgatgatccg
     5881 gcatgagatt gttttatcgt cagtttgtcg ccgccgttat cttttgccca ttgttgctca
     5941 aacggcggat tcagggcggc aaacagctcg cgggagacgt cataagaact gttcagcagt
     6001 tccgttgcct gtacatggcc cgccagcagc agagaagcga ccagcgcgag tgagttcttt
     6061 ttcagtaagt taacggccat tgcgcaccct tattaattaa atgactttct aatagccatc
     6121 atatttataa cggacgtgaa aggagtaacg gttttatata ccgtttggtg atttggaagt
     6181 tgaaaaggga ataagacgaa atcctcccca caaatgcagg gaggaaacag aggtgaatca
     6241 gatgccgacg ctaaccgtct ccggcagtgt gctgccgcca tcaatcacat tctgtgtacc
     6301 ggttaaatag ctggattcat ccgatgcgag gaaggccgcc agttcgccga cttccagcgg
     6361 atcggcgagg cgacgcatcg ggattgcttt cgccatttca gtcagcaccg attctggatc
     6421 ttccgggttc gactggcggg caatgctttc cgccattggc gtgcgaacgt atccggggca
     6481 gatggcgtta acgcgaatac cagactgcgc gtactccacc gccagcgatt tagtcaggcc
     6541 aacaatcgcc gctttcgtta aggcatacgc cgtttcaccc ggatcggcca ccatatcgcc
     6601 agtgactgaa gacatcatca caatgcgacc atctttgcgg gcaatcatct ccggcaacac
     6661 cgccttcgtg acgttccata cgccttttat attgatatca atatggaaat cgcgatcttc
     6721 atcgctcata tcgaggaaac ttcccagacg acaaacgcct gcgttattca ccaggatatc
     6781 aatgcgccct tctttttcct tcgcacgctt gatagctgcg gcgaccgacg ccgggtcacg
     6841 cacatcggcg acaaccgccg tacagcgatg accacgacca cacagttcgt ccgccagctt
     6901 ttcgatctca ggggagatat ccagcaagat taggtttgcg ccatgacgcg caaaagttct
     6961 ggcaattcct tcgccaattc cctgcaatgc gcccgtaatc agtgctgtct tgcccgtgag
     7021 tttacccatt ttaatgctct ccttgttaat acagagcgtt acactccatt ccctctgaaa
     7081 atacaggata aacaaaatat tgctgcggat cagctcacta attcaggaaa atctcaatcc
     7141 agtcgctgct ttatgcatca tttcaggcgt tgcgttagct cactgctgcg ctcaatcatt
     7201 ttcaatgact ccacatcatt gagttgcaca aggccaacaa acagtaaatc ggtaacagaa
     7261 ttttgcgccg tgcgggtgga catcgacgag ctgcgccatt cggtttcgcc agaaacggta
     7321 tcgagggtgt aatgcgccag ccgccgcagc ggggaatcgg ttaatgaggt aatggcaatc
     7381 accgtcgccc cctgctttcg cgctgcctcg gcgcacaaga caatctcttt tttactgcca
     7441 ctgtaagaga tagcgatttg tacatcgcct ttttttagcg cctgagaaac ggtggcttgt
     7501 acgtgggtgt cggcctcaca ggccacgcga taaccaattt tcatcagttt gaacgacaga
     7561 tcgcgcccca ccagcgccga cccgcccagg cccgttatct ggataaacgg tgctttactg
     7621 atgacctcaa tgattttttg cagccgcgcg tagtcaaaca acgcgcaggt ttgctccagc
     7681 gcctgctctt tttcgcgatt cagtttacgg gcaatcactt ccagcgaatc atcactggta
     7741 atcgaactgt gcaggtgcag cgccgtggca ttcgttttct cccggctggc actgtattcg
     7801 ccaattaacg ccatacgcaa ttcagtaaat ccttgcgcgc cgagcttctg ggcaaacttc
     7861 acaatactcg actggctgat gccgagctgt ttcgccatct tgcgcgaaga gacggatttc
     7921 agctcactca cgttggcccg cagaaagtca gcaatttttt gctcgttccc ggtaaattca
     7981 ctttccgcat tgcgtatttt cgtcaggtac aacatcttcc actctcctgg gctggcagca
     8041 aaaattccgc ttaagttctt atgacgctct tcacactctg cgagtgtaga ctcaatgatt
     8101 cctttaagtc tgtcaattcg ggatatatga ttccatacat ctgcaattat ttgatcgtaa
     8161 atagtaaggt caccaccgat gcaacttgaa aagatgatta ctgaaggctc gaatgccgcc
     8221 tcggctgaaa ttgaccgcgt atcaacgctg gaaatgtgcc ggattatcaa cgatgaagat
     8281 aaaaccgtgc cgcttgccgt tgagcgcgta ctgccggata tcgccgcggc aatcgatgtt
     8341 atccacgccc aggtaagcgg cggcggtcgt ctgatttatc tcggtgcggg aacatccggt
     8401 cgtctgggga ttcttgatgc cagcgaatgt ccgccgacct acggcgtgaa accgggtttg
     8461 gtggttggtt tgattgctgg aggcgaatat gccattcagc acgcggtgga aggcgctgaa
     8521 gatagccggg aaggtggtgt taacgatctg aaaaacatcg gcttaaccgc gcaggatgtg
     8581 gtggttggca ttgcggccag tggtcgcacg ccgtatgtaa ttgccggact ggaatacgca
     8641 caccagctcg gctgccgcac agtgggaatt tcctgtaatc cagggagcgc ggtttccacc
     8701 accgctgagt ttgctattac gccggtggtt ggtgccgaag tggttaccgg ttcttcgcga
     8761 atgaaagcag gcacagcgca gaaactggtg ctcaatatgc tctctaccgg gctgatgatt
     8821 aaatccggca aagtgttcgg caacctgatg gtcgatgtgg tcgccaccaa cgaaaaattg
     8881 catgtgcgcc aggtcaatat cgttaaaaac gccaccggat gtagcgccga acaagcggaa
     8941 acagcgttaa ttgcttgcaa gcgcaactgt aaaacggcca ttgtgatggt actgaaaaat
     9001 ctcgatgccg atgaagctaa aaaatgcctg gatcaacacg gcggttttat tcgcaaggct
     9061 ctggaaaagg aataacccat ggctaaagag atcagcagtg aacttctgaa caccattctt
     9121 acccgggtcg gcggaccggg aaatatcgcc agttgtggta actgtatgac gcgcctgcgt
     9181 ctgggtgtac atgacagttc actggttgat cccaatatca aaacgctgga aggcgtgaag
     9241 ggggtcattt tgaccagcga tcaggtacag gtggtttttg ggccaggcaa agcgcatcgt
     9301 gccgcaaaag ccatgagcga gttgctgggc gaagcaccga tacaggatgc cgcagagatc
     9361 gccgctcaga ataaacgtca gttaaaagcc aaacaaacct ccggcgtgca acaatttctc
     9421 gccaaattcg ccaccatctt cacgccgctg atccccggtt ttattgccgc tggtctgttg
     9481 ctgggaatag cgacgttaat tgccacggtg atgcacgttc cggcagatgc tcagggaaca
     9541 ctacccgatg cgctgaattt tatgaaggtg ttcagcaaag gcctgttcac cttcctggtg
     9601 attctggtgg gctataacgc ggcccaggca ttcggcggca cgggcgtcaa tggcgcaatt
     9661 atcgccgcgc tctttttgct cggttataac ccagctgcaa ccacgggtta ctacgccgga
     9721 tttcacgatt tctttggtct gcccatcgat ccgcgcggca atattatcgg cgtgttgatt
     9781 gccgcgtggg cctgcgcccg catcgaaggc atggtgcgcc gctttatgcc cgacgatctc
     9841 gacatgctgt tgacctcgtt aatcaccctg ctaatcaccg ctacgcttgc gtacctgatc
     9901 attatgccgc tgggcggctg gttattcgaa ggtatgtcgt ggctgtttat gcacctgaac
     9961 agtaatccgc tcggttgtgc ggttttagcc gggctattcc tgatcgccgt ggtatttggc
    10021 gtacatcagg gctttattcc tgtttacctc gcgttaatgg acagtcaggg attcaacagc
    10081 ttatttccca tcctttcaat ggcaggcgcg ggccaggtgg gcgcggcgct ggcactctac
    10141 tggcgggcgc aaccgcatag cgcattacgc agtcaggtcc gcggggcgat tattcctggt
    10201 ctgctgggcg ttggcgaacc gctgatttac ggcgtcaccc tgccccgcat gaaaccgttt
    10261 gttaccgctt gcttaggcgg cgcggcgggc ggtttgttta tcggactgat agcctggtgg
    10321 ggtctgccga tgggcttaaa cagcgccttt gggccgtctg gtctggtggc gctgccgctg
    10381 atgacctcag cacaaggtat ccttcctgca atggcggttt atgcaggcgg gattctggtg
    10441 gcatgggttt gcgggtttat tttcaccacg ctctttggct gccgtaacgt caatctggac
    10501 tgataatatg aaacggacaa tgctctatct ttctctgctg gctgtcagct gtagcgtcag
    10561 cgccgcaaaa taccctgttc tgacagaaag ttcgccagag aaggcagggt ttaacgtcga
    10621 acggcttaat cagatggatc gctggataag ccagcaagtt gatgccggtt atcccggcgt
    10681 aaacctgctg attataaaag ataatcagat tgtttatcgc aaagcctggg gcgcggcgaa
    10741 aaagtacgat ggcagcgcgt taatggcgca gcctgtaaaa gccaccaccg ggacgctgta
    10801 tgatctggcc tcaaacacca agatgtacgc cactaacttc gcgctgcaaa agctgatgag
    10861 cgaaggcaaa ctgcatcctg acgatcggat tgcgaaatat attccagggt ttgccgacag
    10921 cccgaacgat gcgatcaaag gcaaaaacac gctgcggatt tctgatctgc tgcatcacag
    10981 cggcggtttc ccggcagatc cgcaataccc gaataaagcg gtcgcgggcg cgttatattc
    11041 ccaggataaa ggccagacgc tggagatgat caagcgcacg ccgctggagt atcagcccgg
    11101 cagcaagcat atctacagcg atgtcgatta tatgctgctt ggatttatcg tcgagtcggt
    11161 caccggtcag ccgcttgacc actatgttga agagtcgatt tatcgcccgc tcggcctgac
    11221 gcatacggtg tttaatccgc tgttgaaagg ctttaaacca cagcaaattg ccgccacgga
    11281 actgaacggc aatacccgcg atggcgtgat tcacttcccg aatatccgca cctccactct
    11341 ctggggtcag gtgcacgatg aaaaagcctt ttattcgatg ggcggtgttt ccgggcacgc
    11401 aggtttgttt tccaataccg gcgatattgc ggtgttaatg caaacgatgc tgaacggcgg
    11461 tggctatggt gatgtgcagc tgttcaatgc agaaacggtg aagatgttca ccaccagctc
    11521 taaggaagat gccacttttg gccttggctg gcgcgtgaat ggtaatgcca ccatgacgcc
    11581 gacgtttggc acgctggcaa gcccgcaaac ctacggtcac acaggttgga ccgggacggt
    11641 gaccgttatc gatccggtga atcatatggc gattgtgatg ttaagcaaca agccacattc
    11701 gccagttgcc gatccacaaa aaaatcccaa tatgttcgaa agcggtcagc taccgattgc
    11761 aacttatggt tgggtagttg atcaggtgta tgcggcgtta aagcagtaat aaaaaggcca
    11821 gtcagttgtg ttactggctg gcctttaatc aatgaatcag aaacgcttac aacgccatca
    11881 acttgtccag cgacggcgca aagtaatagc cgccggttac cggtttggtg aaacgcaaca
    11941 tcgcatcacg cttaccatcg gtatcgccaa acatgctcag cagttgctgc tcaatgttat
    12001 gcagacgcgc gcagtaggcg cagaagtaca gaccgtgagt gccactggcg gtgccgtacg
    12061 gcaggctctg acgaacaatc ttcagccctt tgccatcttc tttcagatca acgcgggtca
    12121 ggtgagaggt ttccggacgt tcgtcgccgt caatctcttc gttggcctct ttggtgcgcc
    12181 cgatcatcat ctcttgatcg tgaacgctca tccgattgag ctgtttcagg ttgtgctccc
    12241 aacgctgcac aaacacatag ctgccgcccg catccacgcc gtctttgata acagccactt
    12301 cgcggcgcgt ctcttcaccc gccgggtttt ctgtaccgtc aacaaagccg ctcaggtcac
    12361 gctcttcaac ccaacggaag ccgtggatct cttctttcac ttcaatgcag tcaccaaagg
    12421 cttccatcgc cgcctgagcg acagagaagt tcacgtcgtg acgcagagaa agaatgtgga
    12481 tcaacacatc gaactgggtc gtcggtgcaa gacctttacc gtagcccgga aaatctttca
    12541 gctcttcggc cccaacgccg ccgctcagag cgcgccaggt attgttacca aaggcaacca
    12601 ccgcaccaag atgcgcgtcc gggaatttcg cttcaaaagt tgccagtttg tcggcaaatg
    12661 ttttactggc cgcacgcagg gcgtcaactt cccctttcac gttggcttcg atccaaatcg
    12721 ccgcgcggca atgttctggc aaaatgccac tctgaacctg agacattgtt cctcctgaaa
    12781 ataataatgc cacgcaatcc gtggcattgt tgtgcgctat tttacgtgtt ttttcgcggc
    12841 ggcggtttgt tcagacgcaa attaacgccg ccagataatt ttactgactt tccagttttt
    12901 cagggtatcg tccgaaggca ttaacccttc agggccactc cattccccgc tgaactggta
    12961 gctaatatgt tgactgcctt cggctttaca ttcgacggca cgattatcat cactgtcggc
    13021 cttttggcaa ttgccaaatg ctttgctgta aaggtcacta aacggtgtac cgattttaac
    13081 accggtgtca gcaggaatat cgctatccag cacatcaatg cggctgatcg tgccttgatc
    13141 accgttaatc accatcgcca cgttgtcgcc tttcatcact tcaaaaaagc gcaccacgtt
    13201 gccgttcgcg gttttcattc cgctgcgcag gcgataatcg ccatcaagcg catcagcaat
    13261 ggcttgttct tgcagtggtg tggacgccgt taattcaccc acgccctgct cgctcacttt
    13321 ggtggatgat ccaaaccagt tccacgggtt agcggccgac cagttaactg acgacatcgt
    13381 ggaacagccg gtcagcatca acggcatagc gcataacatt aaacgcagcg atttcatgtc
    13441 acttcctttg gttattcaat aacgttgctt ggagtgcaaa ttcaccaaaa agtgccgtta
    13501 ttctttttct tcattaaaac aagcgcgtaa acgtcgattg gtcagcagcc agattaacgc
    13561 cacgatatcc gccactacca gcgccagacc gataccgcta acggattcac cgttcagcca
    13621 cagccacggt tgccaacaaa gtaaaaccac ctgcgccaac agcaacagaa aatagagcac
    13681 atgccagata cggggaaatg aagcccgccg accactcagc aaaaatgcca gcaccgccgg
    13741 aataccggga atcaacccca gccagaaatt atcgtgatcg ggataaaaca gatttagcag
    13801 cgcagtaccc tgctcgcgcg acgcaccggc aatgacaaac agcacccagg ttcgcgcctg
    13861 aagcaatagc acaagccaga agagcaaggg taaacgcagg cgaccgtgcg catcataatg
    13921 gacaggatga aactcagtac tcttcatctt caatcaaacg cttacccaga ctcagcacgt
    13981 cggcgtgttc atatcccagg cgttcataca ttccgagcac catgtcgtta tcttccggca
    14041 cgttgatctg aattttcgga cagccgcgag caatcagctt tttctccaac cgattaagca
    14101 acgcattagc aatcccacgc ccacgaaact ctggatgcac gccaagataa taagcagacc
    14161 cgcgatgccc gtcataaccg cccatcaccg ttccgaccac ttcaccgttt acctcagcga
    14221 ccagaaacaa actgacgtca tggttcatct tacgctcgat gtccatttcc ggatcgttcc
    14281 acggacgcag caagtcgcaa cgctcccaaa gggtgatgac ctcttcgaaa tcttcctggc
    14341 gaaatacgcg tatctccatg gtattcgtta cctttttgcg ggttaaaagg ctgattatgg
    14401 cgtgaacggt cgaattagcc aatatctgac gaaaatcggt tgaaaaagtg gcataatggg
    14461 gagttgtcaa ctattgaaat gaaaagtaaa acaattctca acagcaaacc gtcgtaacgg
    14521 attacgcgat acgatataac atctggaact ttattattac aactcaggcc gtatgagcac
    14581 ttttaaacca ctaaaaacac tcacttcgcg ccgc
//
LOCUS       DAMZFT010000125        14113 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.125, whole genome shotgun sequence.
ACCESSION   DAMZFT010000125 DAMZFT010000000
VERSION     DAMZFT010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14113)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14113)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14113
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.125"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..799
                     /locus_tag="PN977_003904"
     CDS             <1..799
                     /locus_tag="PN977_003904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HDI6043897.1"
                     /translation="ESRLPGILELSRTFAHVDPVKEPIPVIPTCHYMMGGIPTKVTGQ
                     ALTVNEKGEDVVVPGLFAVGEIACVSVHGANRLGGNSLLDLVVFGRAAGLHLQESIAE
                     QGALRDASESDVEASLDRLNRWNNNRNGEDPVAIRKALQECMQHNFSVFREGDAMAKG
                     LEQLKVIRERLKNARLDDTSSEFNTQRVECLELDNLMETAYATAVSANFRTESRGAHS
                     RFDFPDRDDENWLCHSLYLPESESMTRRSVNMEPKLRPAFPPKIRTY"
     gene            815..1531
                     /gene="sdhB"
                     /locus_tag="PN977_003905"
     CDS             815..1531
                     /gene="sdhB"
                     /locus_tag="PN977_003905"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit
                     SdhB"
                     /protein_id="HDI6043898.1"
                     /translation="MRLEFSIYRYNPDVDDAPRMQDYTLEAEEGRDMMLLDALIQLKE
                     KDPSLSFRRSCREGVCGSDGLNMNGKNGLACITPISALNQPGKKIVIRPLPGLPVIRD
                     LVVDMGQFYAQYEKIKPYLLNNGQNPPAREHLQMPEQREKLDGLYECILCACCSTSCP
                     SFWWNPDKFIGPAGLLAAYRFLIDSRDTETDSRLDGLSDAFSVFRCHSIMNCVSVCPK
                     GLNPTRAIGHIKSMLLQRNA"
     gene            1732..4533
                     /gene="sucA"
                     /locus_tag="PN977_003906"
     CDS             1732..4533
                     /gene="sucA"
                     /locus_tag="PN977_003906"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HDI6043899.1"
                     /translation="MQNSALKAWLDSSYLSGANQSWIEQLYEDFLTDPDSVDANWRST
                     FQQLPGTGVKPDQFHSQTREYFRRLAKDASRYSSTISDPDTNVKQVKVLQLINAYRFR
                     GHQHANLDPLGLWQQDKVADLDPSFHDLTEADFQETFNVGSFASGKETMKLGELLEAL
                     KQTYCGPIGAEYMHITSTEEKRWIQQRIESGRATFNSEEKKRFLSELTAAEGLERYLG
                     AKFPGAKRFSLEGGDALIPMLKEMIRHAGNSGTREVVLGMAHRGRLNVLVNVLGKKPQ
                     DLFDEFAGKHKEHLGTGDVKYHMGFSSDFQTDGGLVHLALAFNPSHLEIVSPVVIGSV
                     RARLDRLDEPSSNKVLPITIHGDAAVTGQGVVQETLNMSKARGYEVGGTVRIVINNQV
                     GFTTSNPLDARSTPYCTDIGKMVQAPIFHVNADDPEAVAFVTRLALDFRNTFKRDVFI
                     DLVCYRRHGHNEADEPSATQPLMYQKIKKHPTPRKIYADKLEQEKVATLEDATEMVNL
                     YRDALDAGDCVVAEWRPMNMHSFTWSPYLNHEWDEEYPNKVEMKRLQELAKRISTVPE
                     AVEMQSRVAKIYGDRQAMAAGEKLFDWGGAENLAYATLVDEGIPVRLSGEDSGRGTFF
                     HRHAVIHNQSNGSTYTPLQHIHNGQGAFRVWDSVLSEEAVLAFEYGYATAEPRTLTIW
                     EAQFGDFANGAQVVIDQFISSGEQKWGRMCGLVMLLPHGYEGQGPEHSSARLERYLQL
                     CAEQNMQVCVPSTPAQVYHMLRRQALRGMRRPLVVMSPKSLLRHPLAVSSLEELANGT
                     FLPAIGEIDELDPKGVKRVVMCSGKVYYDLLEQRRKNNQHDVAIVRIEQLYPFPHKAM
                     QEVLQQFAHVKDFVWCQEEPLNQGAWYCSQHHFREVIPFGASLRYAGRPASASPAVGY
                     MSVHQKQQQDLVNDALNVE"
     gene            4548..5765
                     /gene="odhB"
                     /locus_tag="PN977_003907"
     CDS             4548..5765
                     /gene="odhB"
                     /locus_tag="PN977_003907"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HDI6043900.1"
                     /translation="MSSVDILVPDLPESVADATVATWHKKPGDAVVRDEVLVEIETDK
                     VVLEVPASADGILDAVLEDEGTTVTSRQILGRLREGNSAGKETSAKSEEKASTPAQRQ
                     QASLEEQNNDALSPAIRRLLAEHNLDASAIKGTGVGGRLTREDVEKHLAKAPAKESAP
                     AAAAPAAQPALAARSEKRVPMTRLRKRVAERLLEAKNSTAMLTTFNEVNMKPIMDLRK
                     QYGEAFEKRHGIRLGFMSFYVKAVVEALKRYPEVNASIDGDDVVYHNYFDVSMAVSTP
                     RGLVTPVLRDVDTLGMADIEKKIKELAVKGRDGKLTVEDLTGGNFTITNGGVFGSLMS
                     TPIINPPQSAILGMHAIKDRPMAVNGQVEILPMMYLALSYDHRLIDGRESVGFLVTIK
                     ELLEDPTRLLLDV"
     gene            5859..7025
                     /gene="sucC"
                     /locus_tag="PN977_003908"
     CDS             5859..7025
                     /gene="sucC"
                     /locus_tag="PN977_003908"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HDI6043901.1"
                     /translation="MNLHEYQAKQLFARYGLPAPVGYACTTPREAEEAASKIGAGPWV
                     VKCQVHAGGRGKAGGVKVVNSKEDIRAFAENWLGKRLVTYQTDANGQPVNQILVEAAT
                     DIAKELYLGAVVDRSSRRVVFMASTEGGVEIEKVAEETPHLIHKVALDPLTGPMPYQG
                     RELAFKLGLEGKLVQQFTKIFMGLATIFLERDLALIEINPLVITKQGDLICLDGKLGA
                     DGNALFRQPDLREMRDQSQEDPREAQAAQWELNYVALDGNIGCMVNGAGLAMGTMDIV
                     KLHGGEPANFLDVGGGATKERVTEAFKIILSDDKVKAVLVNIFGGIVRCDLIADGIIG
                     AVAEVGVNVPVVVRLEGNNAELGAKKLADSGLNIIAAKGLTDAAQQVVAAVEGK"
     gene            7025..7894
                     /gene="sucD"
                     /locus_tag="PN977_003909"
     CDS             7025..7894
                     /gene="sucD"
                     /locus_tag="PN977_003909"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HDI6043902.1"
                     /translation="MSILIDKNTKVICQGFTGSQGTFHSEQAIAYGTKMVGGVTPGKG
                     GTTHLGLPVFNTVREAVAATGATASVIYVPAPFCKDSILEAIDAGIKLIITITEGIPT
                     LDMLTVKVKLDEAGVRMIGPNCPGVITPGECKIGIQPGHIHKPGKVGIVSRSGTLTYE
                     AVKQTTDYGFGQSTCVGIGGDPIPGSNFIDILEMFEKDPQTEAIVMIGEIGGSAEEEA
                     AAYIKEHVTKPVVGYIAGVTAPKGKRMGHAGAIIAGGKGTADEKFAALEAAGVKTVRS
                     LADIGEALKTVLK"
     gene            8560..9465
                     /locus_tag="PN977_003910"
     CDS             8560..9465
                     /locus_tag="PN977_003910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDI6043903.1"
                     /translation="MRGKIPKTELLVTFEVVARHESYTRAAEELALTQSAVFRQVSAL
                     EEFLHTPLFSHSKKRIFLNDAGKYYLGIVKETLNKLERDTNTIMTWQPTVQVIELAVN
                     PTFSTHWLIPNLHEFTKLHPDIIVNIHSLANNGDFLNREYDAVIMRENFCAPWAEVEY
                     LFDEEILPVCSGSLLAMSDQKLSVAELLTELPLLHQSTRITGWEEWFALSGVSSPLVN
                     NGPRFDLLSMLIAAVRSNLGVALLPRFAIQHDLDSGDMVIPCDVPIRTGNRFIMTWQE
                     EKSDSPHLQQFREWLLAKSVVPQEM"
     gene            complement(9499..10101)
                     /gene="cobO"
                     /locus_tag="PN977_003911"
     CDS             complement(9499..10101)
                     /gene="cobO"
                     /locus_tag="PN977_003911"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308783.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HDI6043904.1"
                     /translation="MEARISTERHVQRQQKLKEQVDTRVAAAIEKKGILIVFTGNGKG
                     KSTAAFGTVTRAVGHGKTVGVAQYIKGQWDNGEYNLLQPLGVEFHIMGTGFTWETQNR
                     QADIDAAKEVWSESKRMLADKRYDLVVLDELTYMLAYHYLDTEEVIASLQNRPAQQSV
                     IVTGRGCHSQILKMADTVSEIRPVKHAFDNGIQAQPGIDW"
     gene            complement(10111..11763)
                     /locus_tag="PN977_003912"
     CDS             complement(10111..11763)
                     /locus_tag="PN977_003912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000042471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HDI6043905.1"
                     /translation="MSKPFIWQEPFLQNKDGTEYTLISDQHITVTELDGEEVIKIAPE
                     ALTLLARQAFYEASFFLRSAHLQQVASILNDPHASSNDKYVALQLLRNAEVSAKGVLP
                     NCQDTGTATIVASKGQQIWTGGNDAEALSKGIYTTFQENNLRFSQNAPLDMFTEVNTQ
                     TNLPAQIDISAVAGDEYHFLCINKGGGSANKAALYQETKSLLQPEKLTAFLIEKMKSL
                     GTAACPPYHIAFVVGGLSADQTLKVAKLASTKYYDNLPTSGNEQGQAFRDIELEKVLL
                     EASQQFGIGAQFGGKYFAHDIRVIRLPRHGGSCPIAMALSCSADRNIKAKINKHGIWL
                     EKLEHNPGQYIPASLREENHAQHVQLDLNRPLRDVMQDLARLPVGTRVSLSGPIVVAR
                     DIAHAKIKARLDSGEPMPDYLKHHIVYYAGPAKTPENMACGSLGPTTGGRMDGYVDTF
                     QAAGGSLVMLSKGNRSQQVTDACHKHGGFNLGSIGGAAALLAQEYVKSLRCLEYPELG
                     MEAVWMMEVENLPAFILVDDKGNNFFSQFEQQHRCASCPAGH"
     gene            complement(11871..13151)
                     /locus_tag="PN977_003913"
     CDS             complement(11871..13151)
                     /locus_tag="PN977_003913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308785.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HDI6043906.1"
                     /translation="MKKISLTTMILLALVLGMIIGVVLNNTASPETAKLYAQEISIFT
                     TIFLRLIKMIIAPLVVSTLVVGIAKMGDAKALGRIFSKTLFLFICASLLSIALGLITV
                     NFFMPGTGINFVAHGAETTGVVASEPFTLKVFISHAFPTSIVDAMAHNEILQIVVFSI
                     FLGCSLTAIGEKGSAIVHALDSLAHAMLKLTGYVMLFAPLTVFAAISALIAERGLAVM
                     VSAGIFMGEFYFTMLLLWVLLIGLAIVYVGPCIRRLTRALSEPALLAFTTSSSEAAFP
                     GTLEKLEQFGVSPKIASFVLPIGYSFNLVGSMAYCSFATVFIAQACNIHLSIGEQITM
                     LLILMLTSKGMAGVPRASMVVIAATLNQFNIPEAGLILLMGVDPFLDMGRSATNVMSN
                     AMGAAMVSRWEGEHFGEGCRGKALKPNESNVALP"
     gene            complement(13312..13629)
                     /locus_tag="PN977_003914"
     CDS             complement(13312..13629)
                     /locus_tag="PN977_003914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4387 domain-containing protein"
                     /protein_id="HDI6043907.1"
                     /translation="MKQSIVSLAQVIRSKNAGPYELVLDILFKTKENYERVKSSGQLT
                     PELIARLYHVEPDFIHRIVWFDPSNAVKIVMPRDIISGNVGDNDVYGAQQHAPLLNMS
                     FDL"
     gene            complement(13626..>14113)
                     /locus_tag="PN977_003915"
     CDS             complement(13626..>14113)
                     /locus_tag="PN977_003915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="3-methylaspartate ammonia-lyase"
                     /protein_id="HDI6043908.1"
                     /translation="LKLEGARRVGFRCLTIAGTRDPIMIAGIDKIIDEVKTSVSRNLS
                     LDDDSIRINFHLYGKNGVMGDHEPMQTAGHELGIVLDVVAPTQEIANSVCSLVRSTML
                     HYGYENRIATAGNLAFPFSPSDIQGGPVYEFSIYHLIEANDALRFDFHIEQVTPEGVQ
                     A"
BASE COUNT         3474 a         3546 c         3708 g         3385 t
ORIGIN      
        1 cgaatcccgt ctgccgggca tcctggagct ttcccgtacc ttcgctcacg ttgatccggt
       61 gaaagagccg attccggtta tcccaacctg tcactacatg atgggcggta ttccgaccaa
      121 agtgaccggt caggcgctga cggtgaacga gaaaggcgaa gatgtggttg ttccggggct
      181 gtttgccgtt ggtgaaatcg cttgtgtatc ggtacacggc gctaaccgtc tgggcggcaa
      241 ctcgctgctg gacctggtgg tctttggtcg cgcagcgggt ctgcatctgc aagagtctat
      301 cgccgagcag ggcgcactgc gcgatgccag cgagtctgat gtagaagcgt ctctggatcg
      361 cctgaaccgc tggaacaaca accgtaacgg tgaagatccg gtggcgatcc gtaaagcact
      421 gcaagaatgt atgcagcata acttctcggt cttccgtgaa ggtgatgcga tggcgaaagg
      481 tcttgagcag ttgaaagtta tccgcgagcg tctgaaaaat gcccgtctgg atgacacttc
      541 cagcgagttc aacacccagc gcgttgagtg cctggaactg gataacctga tggaaacggc
      601 gtatgcaacg gctgtttctg ccaacttccg taccgaaagc cgtggcgcgc atagccgctt
      661 cgacttcccg gatcgtgatg atgaaaactg gctgtgccac tccctgtatc tgcctgagtc
      721 ggaatccatg acgcgccgaa gcgtcaacat ggaaccgaaa ctgcgcccgg cattcccgcc
      781 gaagattcgt acttactaat gcggagacag gaaaatgaga ctcgagtttt caatttatcg
      841 ctataacccg gatgttgatg atgctccgcg tatgcaggat tacaccctgg aagcggaaga
      901 aggtcgcgac atgatgctgc tggatgcgct tatccagctg aaagagaaag atcccagcct
      961 gtcgttccgc cgctcctgcc gcgaaggtgt gtgcggttcc gacggtctga acatgaacgg
     1021 taagaatggt ctggcctgta ttaccccgat ttcggcactc aaccagccgg gcaagaagat
     1081 tgtgattcgc ccgctgccag gtttaccggt gatccgcgat ttggtggtag acatgggaca
     1141 attctatgcg caatatgaga aaattaagcc ttacctgttg aataatggac aaaatccgcc
     1201 agctcgcgag catttacaga tgccagagca gcgcgaaaaa ctcgacgggt tgtatgaatg
     1261 tattctctgc gcatgttgtt caacctcttg tccgtctttc tggtggaatc ccgataagtt
     1321 tatcggcccg gcaggcttgt tagcggcata tcgtttcctg atcgatagcc gtgataccga
     1381 gactgacagc cgcctcgacg gtttgagcga tgcattcagt gtattccgct gtcacagcat
     1441 catgaactgc gtcagtgtat gtccgaaggg gctgaacccg acgcgcgcca tcggccatat
     1501 caagtcgatg ttgttgcaac gtaatgcgta attaaatgtt gatgccgggg aaatcccccg
     1561 gctattcagg aaatctttaa aaactgcccc tgacactaag acagttttta aaggttcctt
     1621 cgcgagccac tacgtagaca agagctcgca agtgaacccc ggcacgcaca tcattgtgcg
     1681 tggtagtatc cacggcgaag taagcataaa aaagatgctt aagggatcac gatgcagaac
     1741 agcgctttga aagcctggtt ggactcttct tacctctctg gcgcaaacca gagctggata
     1801 gaacagctct atgaagactt cttaaccgat cctgactcgg ttgacgctaa ctggcgttcg
     1861 acgttccagc agttacctgg tacgggagtc aaaccggatc aattccactc tcaaacgcgt
     1921 gaatatttcc gccgcctggc gaaagacgct tcacgttact cttcaacgat ctccgaccct
     1981 gacaccaatg tgaagcaggt taaagtcctg cagctcatta acgcataccg cttccgtggt
     2041 caccagcatg cgaatctcga tccgctggga ctgtggcagc aagataaagt ggccgatctg
     2101 gatccgtctt tccacgatct gaccgaagca gacttccagg agaccttcaa cgtcggttca
     2161 tttgccagcg gcaaagaaac catgaaactc ggcgagctgc tggaagccct caagcaaacc
     2221 tactgcggcc cgattggtgc cgagtatatg cacatcacca gcaccgaaga aaaacgctgg
     2281 atccaacagc gtatcgagtc tggtcgcgcg actttcaata gcgaagagaa aaaacgcttc
     2341 ttaagcgaac tgaccgccgc tgaaggcctt gaacgttacc tcggcgcaaa attccctggc
     2401 gcaaaacgct tctcgctgga aggcggtgac gcgttaatcc cgatgcttaa agagatgatc
     2461 cgccacgctg gcaacagcgg cacccgcgaa gtggttctcg ggatggcgca ccgtggtcgt
     2521 ctgaacgtgc tggtgaacgt gctgggtaaa aaaccgcaag acttgttcga cgagtttgcc
     2581 ggtaaacata aagaacacct cggcacgggc gacgtgaaat accacatggg cttctcgtct
     2641 gacttccaga ccgatggcgg cctggtgcac ctggcgctgg cgtttaaccc gtctcacctt
     2701 gagattgtca gcccggtagt tatcggttct gttcgtgccc gtctggacag actcgatgag
     2761 ccgagcagca acaaagtgct gccaatcacc atccacggtg acgccgcagt gaccgggcag
     2821 ggcgtggttc aggaaaccct gaacatgtcg aaagcgcgtg gttatgaagt tggcggtacg
     2881 gtacgtatcg ttatcaacaa ccaggttggc ttcaccacct ctaatccgct ggatgctcgt
     2941 tctacaccgt actgtactga tatcggtaag atggttcagg caccgatttt ccacgtaaac
     3001 gcggatgatc cggaagccgt tgcttttgtg acccgtctgg cgctcgattt ccgtaacacc
     3061 tttaaacgtg atgtcttcat cgacctggtg tgctaccgcc gtcacggcca caacgaagcc
     3121 gacgagccga gcgcaaccca gccgctgatg tatcagaaaa tcaaaaaaca tccgacgccg
     3181 cgcaaaatct atgctgacaa gctggagcag gaaaaagtcg cgacgctgga agatgccacc
     3241 gagatggtta acctgtaccg cgatgcgctg gatgctggcg attgcgttgt agcagagtgg
     3301 cgtccgatga acatgcactc tttcacctgg tcgccgtacc tcaaccacga atgggacgaa
     3361 gagtacccga acaaagttga gatgaagcgc ctgcaggaac tggctaaacg catcagcacg
     3421 gtgccggaag cagttgaaat gcagtctcgc gttgccaaga tttatggcga tcgccaggcg
     3481 atggctgctg gtgagaaact gttcgactgg ggcggcgcgg aaaacctcgc ttacgccacg
     3541 ctggttgacg aaggcattcc ggttcgcctg tcgggtgaag actccggtcg cggtaccttc
     3601 ttccaccgcc acgcggtgat ccacaaccag tctaacggtt ccacttacac gccgctgcaa
     3661 cacatccata acggccaggg cgcgttccgt gtctgggact ccgtactgtc tgaagaagcc
     3721 gtactggcgt ttgaatacgg ttatgccacc gcagaaccac gcactctgac tatctgggaa
     3781 gcgcaattcg gtgacttcgc caacggtgct caggtggtta tcgaccagtt catctcctct
     3841 ggcgagcaga aatggggccg gatgtgtggt ctggtgatgt tgctgccgca cggttacgaa
     3901 gggcaggggc cggagcactc ctccgcgcgt ctggaacgtt atctgcaact ttgcgctgag
     3961 caaaacatgc aggtgtgcgt accgtctacc ccggcacagg tttaccacat gctgcgtcgt
     4021 caggcgctgc gcgggatgcg tcgtccgctg gtcgtgatgt cgccgaaatc cctgctgcgt
     4081 catccgctgg cggtttccag cctcgaagaa ctggcgaacg gcaccttcct gccagccatc
     4141 ggtgaaatcg acgagcttga tccgaagggc gtgaagcgcg tagtgatgtg ttctggtaag
     4201 gtttattacg acctgctgga acagcgtcgt aagaacaatc aacacgatgt cgccattgtg
     4261 cgtatcgagc aactctaccc gttcccgcat aaagcgatgc aggaagtgtt gcagcagttt
     4321 gctcacgtca aggattttgt ctggtgccag gaagagccgc tcaatcaggg cgcatggtac
     4381 tgcagccagc atcatttccg tgaagtgatt ccgtttgggg cttctctgcg ttatgcaggc
     4441 cgcccggcct ccgcctctcc ggcggtaggg tatatgtccg ttcaccagaa acagcaacaa
     4501 gatctggtta atgacgcgct gaacgtcgaa taaataaagg atacacaatg agtagcgtag
     4561 atattctggt ccctgacctg cctgaatccg tagccgatgc caccgtcgca acctggcata
     4621 aaaaacccgg cgacgcagtc gtacgtgatg aagtgctggt agaaatcgaa actgacaaag
     4681 tggtactgga agtaccggca tcagcagacg gcattctgga tgcggttctg gaagatgaag
     4741 gtacaacggt aacgtctcgt cagatccttg gtcgcctgcg tgaaggcaac agcgccggta
     4801 aagaaaccag cgccaaatct gaagagaaag cgtccactcc ggcgcaacgc cagcaggcgt
     4861 ctctggaaga gcaaaacaac gatgcgttaa gcccggcgat ccgtcgcctg ctggctgaac
     4921 acaatcttga tgccagcgcc attaaaggca ccggtgtagg tggtcgtctg acccgtgaag
     4981 atgtggaaaa acatctggcg aaagccccgg cgaaagagtc tgctccggca gcggctgctc
     5041 cggcggcgca accggccctg gctgcacgta gcgaaaaacg tgtgccgatg actcgcctgc
     5101 gtaagcgtgt ggcagagcgt ctgctggaag cgaaaaactc caccgccatg ctgaccacgt
     5161 tcaacgaagt caacatgaag ccgattatgg atctgcgtaa gcagtacggt gaagcgtttg
     5221 aaaaacgcca cggcatccgt ctgggcttta tgtccttcta cgtgaaagcg gtggttgaag
     5281 ccctgaaacg ttacccggaa gtgaatgctt ctatcgacgg cgatgacgtg gtttaccaca
     5341 actatttcga cgtcagcatg gcggtttcta cgccgcgcgg cctggtgacg ccggtactgc
     5401 gtgatgtcga taccctcggt atggcagaca ttgagaagaa aattaaagag ctggcagtta
     5461 aaggccgtga cggcaagctg acggttgaag atctgaccgg tggtaacttc accatcacca
     5521 acggtggtgt gttcggttcc ctgatgtcta cgccgatcat caacccgccg cagagcgcaa
     5581 ttctgggtat gcacgctatc aaagatcgtc cgatggcggt gaatggtcag gttgagatcc
     5641 tgccgatgat gtacctggcg ctgtcctacg atcaccgtct gatcgatggt cgcgaatccg
     5701 tgggcttcct ggtaacgatc aaagagttgc tggaagatcc gacgcgtctg ctgctggacg
     5761 tgtagtagtt taagtttcac ctgcactgta gaccgacgtg gatagggtgt cggtctacgg
     5821 tttaaaagat aacgattact gaaggatgga cagaacacat gaacttacat gaatatcagg
     5881 caaaacaact ttttgcccgc tatggcttac cagcaccggt gggttatgcc tgtactactc
     5941 cgcgcgaagc agaagaagcc gcttcaaaaa tcggtgccgg tccgtgggta gtgaaatgtc
     6001 aggttcacgc tggtggccgc ggtaaagcgg gcggtgtgaa agtggtaaac agcaaagaag
     6061 acatccgtgc ttttgcagaa aactggctgg gcaagcgtct ggtaacgtat caaacagatg
     6121 ccaatggcca accggttaac cagattctgg ttgaagcagc gaccgatatc gctaaagagc
     6181 tgtatctcgg tgccgttgta gaccgtagtt cacgtcgcgt ggtctttatg gcctccaccg
     6241 aaggcggcgt ggaaatcgaa aaagtggcgg aagaaactcc gcacctgatc cacaaagttg
     6301 cgcttgatcc gctgactggc ccgatgccgt atcagggacg cgagctggcg ttcaaactgg
     6361 gtctggaagg taaactggtt cagcagttca ccaaaatctt catgggcctg gcgaccattt
     6421 tcctggagcg cgacctggcg ctgatcgaaa tcaacccgct ggtcatcacc aaacagggcg
     6481 atctgatttg cctcgacggc aaactgggcg ctgatggcaa cgcactgttc cgccagcctg
     6541 atctgcgcga aatgcgtgac cagtcgcagg aagatccgcg tgaagcacag gctgcacagt
     6601 gggaactgaa ctacgttgcg ctggacggta acatcggttg tatggttaac ggcgcaggcc
     6661 tggcgatggg caccatggac atcgttaaac tgcacggcgg cgaaccagct aacttcctgg
     6721 acgttggcgg cggcgcaacc aaagagcgcg taaccgaagc gttcaaaatc atcctctctg
     6781 acgacaaagt gaaagccgtt ctggttaaca tcttcggcgg tatcgttcgt tgcgacctga
     6841 tcgctgacgg tatcatcggc gcggtagcgg aagtgggtgt taacgtaccg gtcgtggtac
     6901 gtctggaagg taacaacgcc gaactcggcg cgaagaaact ggctgacagc ggcctgaata
     6961 ttattgcagc aaaaggtctg acggatgcag ctcagcaggt tgttgccgca gtggagggga
     7021 aataatgtcc attttaattg ataaaaacac caaggttatc tgccagggct ttaccggtag
     7081 ccaggggact ttccactcag aacaggctat tgcatacggc actaaaatgg ttggcggcgt
     7141 aaccccaggt aaaggcggca ccacccacct cggcctgccg gtgttcaaca ccgtgcgtga
     7201 agctgttgct gctactggcg ctaccgcttc tgttatctac gtaccagcac cgttctgcaa
     7261 agactccatt ctggaagcta ttgatgcagg catcaaactg attatcacca tcactgaagg
     7321 catcccgacg ctggatatgc tgaccgtgaa ggtgaaactg gatgaagcgg gcgttcgcat
     7381 gatcggcccg aactgcccgg gcgttatcac tccgggtgaa tgcaaaatcg gtatccagcc
     7441 tggtcacatt cacaaaccgg gtaaagtggg tatcgtttcc cgttccggta cactgaccta
     7501 tgaagcggtt aaacagacca cggattacgg tttcggtcag tcgacctgtg ttggtatcgg
     7561 cggcgacccg atccctggct ctaactttat cgacatcctc gaaatgttcg aaaaagatcc
     7621 gcagaccgaa gcgatcgtga tgatcggtga gatcggcggt agcgctgaag aagaagcggc
     7681 tgcgtacatc aaagagcacg ttaccaagcc tgttgtgggt tacatcgctg gtgtgactgc
     7741 gccgaaaggc aaacgtatgg gccacgcggg tgccatcatt gccggtggga aagggactgc
     7801 ggatgagaaa tttgctgctc tggaagccgc aggcgtgaaa accgttcgca gcctggcgga
     7861 tatcggtgaa gcactgaaaa ctgttctgaa ataaatatct gtaataagaa atagccctcg
     7921 ccgcttccct ctacaggaat ggcgaagggc tgtcggtttc gacatggttg gccatcatat
     7981 gatggccttt tttatgcctt aaacgttaac cacggtcgga atctgactgc aggcttgact
     8041 gcatgaccac agccactgtg gattgccgcg accgaccgca agccacgaat aagaacgacg
     8101 gcgaatttcg aatcgttacc tgatgcactg gcttcccagc gtttgaccgt agcgttaagt
     8161 aaatcaacaa aagcgatgtt caccgcttat cgtcaagtcg tgccatttgt cagtcaggat
     8221 cgggtcttta ccccggatat tgaagcatcg caaaaattct tagagggata tgctataaat
     8281 caaaaataaa atctatgttg attgtttatc ataatatcca cagatatgat aaatgatatc
     8341 aatttaataa ttttcactta atgtaattcc tgataattcg aaaggtgctg tgcttttcgg
     8401 attattatta tctgattaag acaattgtca cgccaggctt accaggttat aaatatcgaa
     8461 catgattttt ataaaatatt gattgctgct ttggttgcca ttacttagca ataagtaatg
     8521 gttttttgat agtttcaact atttttaagg gaaggcatta tgcgaggaaa aatacctaaa
     8581 acggaactgc tggtgacttt tgaggtcgtc gcacgtcatg aaagttatac cagagcggca
     8641 gaggagttag cgttaaccca aagtgcagta ttcagacaag taagtgcgct ggaggaattt
     8701 ttacatactc cgttattttc acactctaaa aaacgcattt ttcttaatga tgcagggaag
     8761 tattacctgg ggatagtaaa agaaacatta aataagctgg aacgcgatac caataccatt
     8821 atgacctggc agcctacggt tcaggttatt gaacttgccg tgaatcctac gtttagtacc
     8881 cactggttaa tcccaaattt acatgaattc acaaagttac atcccgatat tattgttaat
     8941 attcattctt tagctaataa cggcgatttt cttaatcggg agtacgatgc tgtcattatg
     9001 cgcgaaaatt tttgcgcgcc ctgggcggaa gtagaatatc tctttgatga ggagattttg
     9061 ccggtatgca gcggcagttt gcttgcaatg tccgatcaaa aactgtcagt ggcagaactg
     9121 cttactgagt taccacttct gcatcaaagc acccgtatta ctggctggga agagtggttt
     9181 gctttatctg gcgttagcag tccgctggtc aataacggac cacgtttcga tttgctttca
     9241 atgctgattg ccgccgtgcg ctctaatctc ggcgtggcgc tgttaccacg gttcgctatt
     9301 caacatgatt tggatagtgg cgatatggtg attccctgtg atgtgcctat tcgtaccggt
     9361 aatcgtttta ttatgacgtg gcaggaagag aagtctgact caccgcactt gcagcaattt
     9421 cgtgaatggt tgctggcgaa atcggtcgta ccgcaggaga tgtaaaacct gcgcctggcg
     9481 ggatgccagg cgcagttatt accagtcgat acccggctgg gcctgaatgc cgttatcaaa
     9541 agcatgtttg acggggcgga tttcgctgac cgtatccgcc atttttaata tctgactgtg
     9601 gcaaccgcgt ccggtgacaa tgacactttg ttgtgccggg cggttttgca gagaagcgat
     9661 cacttcttcg gtatccagat aatggtaagc cagcatatag gtcagctcat caagcaccac
     9721 taagtcgtaa cgcttgtcag ccagcatccg tttgctttcc gaccagacct ctttcgccgc
     9781 gtcgatatcc gcttgccggt tctgtgtctc ccaggtaaaa ccggttccca taatatgaaa
     9841 ctcaaccccc agcggctgga gaaggttgta ttcgccgttg tcccattgcc ccttgatgta
     9901 ctgcgcaacc cccaccgttt tgccatgacc caccgcgcgc gtcacagtac caaacgcggc
     9961 agtggatttc cctttgccat tgccggtgaa aacaatcaaa atcccttttt tctctatcgc
    10021 cgccgcgacg cgcgtatcga cctgttcctt caatttctgc tggcgctgca catgccgttc
    10081 agttgaaatt cgcgcttcca ttcattttcc ttaatgtccc gcagggcaac tggcgcagcg
    10141 gtgttgctgc tcaaactgac taaagaaatt attgccttta tcatctacca ggatgaatgc
    10201 gggtaaattt tccacttcca tcatccacac cgcttccata cccagctccg ggtattccag
    10261 gcaacgcaga cttttgacat attcttgcgc cagtaacgca gctgcaccgc caatactccc
    10321 cagattgaag ccgccatgtt tgtgacaggc atcggtgacc tgctggctgc ggttaccttt
    10381 cgataacatc accagactgc cgccagccgc ctggaaggta tcgacataac cgtccattcg
    10441 accgcccgtg gttggaccca gtgaaccgca ggccatgttc tccggtgttt tcgccgggcc
    10501 agcgtaatag acaatatgat gcttcaggta atccggcatc ggttcgccgc tatccagccg
    10561 cgcttttatt ttggcgtggg cgatatcgcg cgccactacg atgggaccac ttaacgacac
    10621 gcgggtgcct accggtaatc tggcgagatc ctgcatcacg tcacgcaacg gacggtttag
    10681 atccagctgt acatgttgcg cgtgattttc ttcccgcagt gaggctggaa tatattgccc
    10741 cggattgtgt tcgagttttt ccagccagat accgtgcttg ttaatttttg ctttgatatt
    10801 gcgatccgca gaacaggaga gcgccatagc tatcgggcaa gagccaccgt gacgcggcag
    10861 acgaatgacg cgaatatcat gagcgaaata tttaccccca aattgcgcac caataccaaa
    10921 ttgctgactg gcctcgagca atactttttc cagctcaata tcacggaatg cttgtccttg
    10981 ctcgtttccg ctggtgggca aattgtcgta atatttcgtt gaggccagct tggcaacttt
    11041 taaggtttga tcggctgaaa gccctccgac gacaaaggcg atgtggtaag gcgggcaggc
    11101 agcggtgccc agcgatttca ttttttcgat taaaaatgcg gtcagttttt ctggttgcaa
    11161 tagggatttg gtttcctgat aaagcgcggc tttattggca gagccgcccc ctttattaat
    11221 acagagaaaa tggtactcat caccagcgac ggcgctgata tcaatctgcg ccgggagatt
    11281 ggtttgcgta ttgacttcgg taaacatatc cagtggcgca ttttgtgaaa agcgcagatt
    11341 attttcctga aaagtggtgt aaatgccttt gcttaaggct tcagcgtcgt tacctccggt
    11401 ccatatctgt tgccctttac tggcaacaat agttgcggtg ccggtatcct gacagttggg
    11461 taatacccct tttgctgaga cttcagcatt acgtaataac tgcaacgcaa cgtatttatc
    11521 attactactg gcgtgcggat cgttaagaat gctggcgacc tgttgcaaat gagcggaacg
    11581 taagaaaaaa gaggcttcat aaaatgcctg ccgggcaagc agcgttaatg cttcaggagc
    11641 gatttttatc acttcttcgc cgtctaattc cgttactgta atatgttgat cgctaattaa
    11701 tgtatattct gtaccgtctt tattttgtag aaacggttct tgccagataa atggtttgga
    11761 catatttcac ttttcacctt aaacgttagt tttacttaat tgccgtctaa cttcatgatc
    11821 cggcttataa aaatgcgtag tcattggctt ctgattgctt aagatccccc tcagggcaga
    11881 gcaacgttcg attcattggg ttttaatgct ttaccccgac agccctcgcc gaaatgttcg
    11941 ccttcccacc gactcaccat cgcagcgccc attgcgttgc tcatgacgtt tgtcgcggaa
    12001 cgccccatat caaggaacgg atcaacgccc atcagcaaga tcagccccgc ttccggaata
    12061 ttgaactggt tgagcgtggc ggcgataacc accattgagg cgcgtggtac gccagccatt
    12121 cctttcgagg tcaacatcag gatcaacagc atggtgattt gctcaccgat ggataaatgg
    12181 atattgcagg cctgggcgat gaaaactgtg gcgaaggagc agtaggccat tgatccaacg
    12241 agattaaatg agtagccaat gggtaagaca aagctggcaa ttttggggga aacgccaaat
    12301 tgctccagtt tttcaagcgt tcccggaaaa gccgcttcag aactggatgt ggtaaatgcc
    12361 agcagggcgg gttccgaaag ggcacgggtc aggcgtctga tgcaggggcc gacataaacg
    12421 atggccagac cgataagcag cacccaaagt aacaacatgg tgaaataaaa ttcacccata
    12481 aagatcccgg cgctcaccat aactgccagt cctcgttcag caatcaatgc tgaaatagcg
    12541 gcgaatacgg tcaggggagc gaagagcatg acgtagccag tgagctttaa catggcatgt
    12601 gccagcgaat ctaaggcgtg aacgatggcg ctgcctttct caccaatcgc cgtcaggcta
    12661 cagccgagga aaattgagaa caccacgatt tgcaaaattt cattgtgcgc catggcatcg
    12721 acaatgctgg tggggaaagc atgcgaaata aatactttta gcgtaaaggg ttctgacgcg
    12781 accactccgg tggtttcggc tccgtgtgca acaaaattaa ttcctgtgcc tggcatgaag
    12841 aaatttactg ttatcaagcc taaggcgatt gacagcaatg aggcgcaaat aaataaaaag
    12901 agtgttttag aaaaaatacg accaagggct ttggcatctc ccattttagc tatacctacc
    12961 accagggtag agaccactaa cggagcgata atcattttta tcagtcgtaa gaaaatcgtc
    13021 gtgaatatcg atatttcttg cgcatagagt tttgcggttt ccggtgaagc agtgttattg
    13081 agcactacgc cgataatcat tccaagtacc agcgccaaaa gaatcatcgt ggttaaactt
    13141 attttcttca ttttttcctc catcaggtgc acggcgataa atcagaatca ggagaagtag
    13201 aagcctgaat agagattcag gcatctacct gctattacaa ccttacaaat agctgaagtg
    13261 ttgaactgca ataagtgacg ggatgtcagt tggctaaaag tattcggatt tttacaaatc
    13321 gaaactcata tttaataaag gtgcatgttg ctgtgcacca tagacatcat tgtcacctac
    13381 attcccggag ataatatctc gtggcatgac gatttttact gcgttagaag gatcaaacca
    13441 gacaatacgg tgaataaagt caggttcaac atgatataag cgcgcaatca gttctggggt
    13501 taattgccct gatgatttaa ctcgctcata gttttctttc gttttaaata aaatatctaa
    13561 aaccagttcg taaggtccgg catttttcga acgaatgact tgcgccagtg agacaattga
    13621 ctgtttcatg cctgaactcc ttctggcgtc acctgttcaa tatggaaatc aaaacgcaga
    13681 gcgtcgttgg cttcaatcag gtgatatatg gaaaattcgt ataccggccc gccctggata
    13741 tcagaagggg agaacgggaa cgccagattg cctgcggtag cgatgcggtt ttcatagccg
    13801 tagtgcagca tggtagagcg caccagcgag caaacgctat tggcaatttc ctgggtcggt
    13861 gcaactacat caaggacaat ccccagctca tgcccggcag tttgcatcgg ttcatggtcg
    13921 cccatcaccc cgtttttgcc gtacaggtgg aaattgatgc gaatgctgtc atcgtcgagc
    13981 gacaggttac gtgaaacgct ggttttgact tcatcgatga ttttatcgat cccggcgatc
    14041 atgatcgggt cgcgcgttcc tgcgatggtc agacagcgga agcccacccg tcgcgcacct
    14101 tccagtttca ggg
//
LOCUS       DAMZFT010000126        13617 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.126, whole genome shotgun sequence.
ACCESSION   DAMZFT010000126 DAMZFT010000000
VERSION     DAMZFT010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13617)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13617)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13617
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.126"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..1384
                     /gene="sbcB"
                     /locus_tag="PN977_003916"
     CDS             <1..1384
                     /gene="sbcB"
                     /locus_tag="PN977_003916"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="exodeoxyribonuclease I"
                     /protein_id="HDI6043909.1"
                     /translation="YETFGTHPALDRPAQFAAIRTDNEFNVIGEPEVFYCKPADDYLP
                     QPGAVLITGITPQEARAKGENEAAFAARIHSLFTVPKTCILGYNNVRFDDEVTRNVFY
                     RNFYDPYAWSWQHDNSRWDLLDVMRACYALRPEGINWPENDDGLPSFRLEHLTKANGI
                     EHSNAHDAMADVYATIAMAKLVKTRQPRLFDYLFTHRNKHKLMALIDVPQMKPLVHVS
                     GMFGAWRGNTSWVAPLAWHPENRNAVIMVDLAGDISPLLELDSDTLRERLYTAKADLG
                     DNAAVPVKLVHINKCPVLAQANTLRPEDAERLGIARQHCLDNLKVLRENPQVRDKVVA
                     IFAEAEPFTPSDNVDAQLYNGFFSDADRAAMKIVLETEPRNLPALDITFVDKRIEKLL
                     FNYRARNFPGTLDDNEQQRWLEHRRQVFTPEFLQGYAEEIQMLAQLYADDKEKVALLK
                     ALWQYAEEIV"
     gene            complement(1427..1654)
                     /locus_tag="PN977_003917"
     CDS             complement(1427..1654)
                     /locus_tag="PN977_003917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HDI6043910.1"
                     /translation="MAIKKLDVVTQVCPFPLIEAKAALAEMASGDELVIEFDCTQATE
                     AIPQWAAEEGHAITDYQQIGDAAWSITVQKA"
     gene            complement(1668..2726)
                     /gene="yeeE"
                     /locus_tag="PN977_003918"
     CDS             complement(1668..2726)
                     /gene="yeeE"
                     /locus_tag="PN977_003918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transport protein YeeE"
                     /protein_id="HDI6043911.1"
                     /translation="MFSMILSGLICGALLGFVMQRGRFCLTGGFRDMYVAKNNRMFYA
                     LLIAISVQSVGVFALIQAGLLTYEAGAFPWLGTVIGGYLFGLGIVLAGGCATGTWYRA
                     GEGLIGSWIALFTYMVMSAVMRSPHASGLNQTLQHYTTEHNSIADTFNLSVWPLVAVL
                     LVITLWVVMKELKKPKLKVATLPPRRTGIAHILFEKRWHPFVTAVLIGLIALLAWPLS
                     EATGRMFGLGITSPTANILQFLVAGDVKYINWGVFLVLGSFVGSFIAAKASREFRVRA
                     ADAQTTLRSGLGGVLMGFGASIAGGCSIGNGLVMTAMMTWQGWIGLVFMILGVWTASW
                     LVYVRPQRKARLATAAAN"
     gene            complement(2905..4263)
                     /gene="plaP"
                     /locus_tag="PN977_003919"
     CDS             complement(2905..4263)
                     /gene="plaP"
                     /locus_tag="PN977_003919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416518.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PlaP"
                     /protein_id="HDI6043912.1"
                     /translation="MSHNVTPNTSRVELRKTLTLVPVVMMGLAYMQPMTLFDTFGIVS
                     GLTDGHVPTAYAFALIAILFTALSYGKLVRRYPSAGSAYTYAQKSISPTVGFMVGWSS
                     LLDYLFAPMINILLAKIYFEALVPSIPSWMFVVALVAFMTAFNLRSLKSVANFNTVIV
                     VLQVVLIAVILGMVVYGVFEGEGAGTLASTRPFWSGDAHVIPLITGATILCFSFTGFD
                     GISNLSEETKDAERVIPRAIFLTALIGGMIFIFATYFLQLYFPDISRFKDPDASQPEI
                     MLYVAGKAFQVGALIFSTITVLASGMAAHAGVARLMYVMGRDGVFPKSFFGYVHPKWR
                     TPAMNIILVGAIALLAINFDLVMATALINFGALVAFTFVNLSVISQFWIREKRNKTLK
                     DHFQYLFLPMCGALTVGALWVNLEESSMVLGLIWAAIGLIYLACVTKSFRNPVPQYED
                     VA"
     gene            complement(4253..4315)
                     /gene="yoeI"
                     /locus_tag="PN977_003920"
     CDS             complement(4253..4315)
                     /gene="yoeI"
                     /locus_tag="PN977_003920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YoeI"
                     /protein_id="HDI6043913.1"
                     /translation="MGQFFAYATVITVKENDHVA"
     gene            complement(4530..5459)
                     /locus_tag="PN977_003921"
     CDS             complement(4530..5459)
                     /locus_tag="PN977_003921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDI6043914.1"
                     /translation="MKPLLDVLMILDALEKEGSFAAASAKLYKTPSALSYTVHKLESD
                     LNIQLLDRSGHRAKFTRTGKMLLEKGREVLHTVRELEKQAIKLHEGWENELVIGVDDT
                     FPFSLLAPLIEAFYQHHSVTRLKFINGVLGGSWDALTQGRADIIVGAMHEPPSSSEFG
                     FSRLGDLEQVFAVAPHHPLAQEEEPLNRRIIKRYRAIVVGDTAQAGASTASQLLDEQE
                     AITVFDFKTKLELQISGLGCGYLPRYLAQRFLDSGALIEKKVVAQTLFEPVWIGWNEQ
                     TAGLASGWWRDEILANSAIAGVYAKSDDGKSAI"
     gene            complement(5505..6329)
                     /locus_tag="PN977_003922"
     CDS             complement(5505..6329)
                     /locus_tag="PN977_003922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDI6043915.1"
                     /translation="MKKVAIVGLGWLGMPLAMSLSARGWQVTGSKTTQDGVEAARMSG
                     IDSYLLRMEPELVCDSDDLDALMDADALVITLPARRSGPGDEFYLQAVQELVDSALAH
                     RIPRIIFTSSTSVYGDAQGTVKETTPRNPVTNSGRVLEELEDWLHNLPGTSVDILRLA
                     GLVGPGRHPGRFFAGKTAPDGEHGVNLVHLEDVIGAITLLLQAPKGGHIYNICAPAHP
                     ARNVFYPQMARLLGLEPPQFRNSLDSGKGKIIDGSRICNELGFEYQYPDPLVMPLE"
     gene            6756..6806
                     /gene="hisL"
                     /locus_tag="PN977_003923"
     CDS             6756..6806
                     /gene="hisL"
                     /locus_tag="PN977_003923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="his operon leader peptide"
                     /protein_id="HDI6043916.1"
                     /translation="MTRVQFKHHHHHHHPD"
     misc_feature    6782..6905
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00514"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="His leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00514"
     gene            6952..7851
                     /gene="hisG"
                     /locus_tag="PN977_003924"
     CDS             6952..7851
                     /gene="hisG"
                     /locus_tag="PN977_003924"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HDI6043917.1"
                     /translation="MTDNTRLRIAMQKSGRLSDDSRELLARCGIKINLHTQRLIAMAE
                     NMPIDILRVRDDDIPGLVMDGVVDLGIIGENVLEEELLNRRAQGEDPRYFTLRRLDFG
                     GCRLSLATPVDEAWDGPLSLNGKRIATSYPHLLKRYLDQKGISFKSCLLNGSVEVAPR
                     AGLADAICDLVSTGATLEANGLREVEVIYRSKACLIQRDGEMEESKQQLIDKLLTRIQ
                     GVIQARESKYIMMHAPTERLDEVIALLPGAERPTILPLAGDQQRVAMHMVSSETLFWE
                     TMEKLKALGASSILVLPIEKMME"
     gene            7857..9161
                     /gene="hisD"
                     /locus_tag="PN977_003925"
     CDS             7857..9161
                     /gene="hisD"
                     /locus_tag="PN977_003925"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HDI6043918.1"
                     /translation="MSFNTIIDWNSCTAEQQRQLLMRPAISASESITRTVNDILDNVK
                     ARGDDALREYSAKFDKTTVTALKVSAEEIAAASERLSDELKQAMAVAVKNIETFHTAQ
                     KLPPVDVETQPGVRCQQVTRPVASVGLYIPGGSAPLFSTVLMLATPARIAGCNKVVLC
                     SPPPIADEILYAAQLCGVQDVFNVGGAQAIAALAFGTESVPKVDKIFGPGNAFVTEAK
                     RQVSQRLDGAAIDMPAGPSEVLVIADSGATPDFVASDLLSQAEHGPDSQVILLTPDAD
                     MAHQVAEAVERQLAELPRAETARQALSASRLIVTNDLAQCVAISNQYGPEHLIIQTRN
                     ARELVDSITSAGSVFLGDWSPESAGDYASGTNHVLPTYGYTATCSSLGLADFQKRMTV
                     QELSKVGFSALASTIETLAAAERLTAHKNAVTLRVNALKEQA"
     gene            9158..10228
                     /gene="hisC"
                     /locus_tag="PN977_003926"
     CDS             9158..10228
                     /gene="hisC"
                     /locus_tag="PN977_003926"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HDI6043919.1"
                     /translation="MSTVTITDLARENVRNLTPYQSARRLGGNGDVWLNANEYPTAVE
                     FQLTQQTLNRYPECQPKAVIENYAQYAGVKAEQVLVSRGADEGIELLIRAFCEPGKDA
                     ILYCPPTYGMYSVSAETIGVECRTVPTLKNWQLDLQGISDKLDGVKVVYVCSPNNPTG
                     QLINPQDFRTLLELTRGKAIVVADEAYIEFCPQASLAGWLAEYPHLAILRTLSKAFAL
                     AGLRCGFTLANEEVINLLMKVIAPYPLSTPVADIAAQALSPQGIVAMRERVAQIIAER
                     EYLMAALKEIPCVEQVFDSETNYILARFKASSAVFKSLWDQGIILRDQNKQPSLSGCL
                     RITVGTREESQRVIDALRAEQV"
     gene            10228..11295
                     /gene="hisB"
                     /locus_tag="PN977_003927"
     CDS             10228..11295
                     /gene="hisB"
                     /locus_tag="PN977_003927"
                     /EC_number="3.1.3.15"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     histidinol-phosphatase/imidazoleglycerol-phosphate
                     dehydratase HisB"
                     /protein_id="HDI6043920.1"
                     /translation="MSQKYLFIDRDGTLISEPPSDFQVDRFDKLAFEPGVIPELLKLQ
                     KAGYKLVMITNQDGLGTQSFPQADFDGPHNLMMQIFTSQGVQFDEVLICPHLPADECD
                     CRKPKVKLVERYLAEQAMDRANSYVIGDRATDIQLAENMGINGLRYDRETLNWPMIGE
                     QLTKRDRYAHVVRNTKETQIDVQVWLDREGGSKINTGVGFFDHMLDQIATHGGFRMEI
                     NVKGDLYIDDHHTVEDTGLALGEALKIALGDKRGICRFGFVLPMDECLARCALDISGR
                     PHLEYKAEFTYQRVGDLSTEMIEHFFRSLSYTMGVTLHLKTKGKNDHHRVESLFKAFG
                     RTLRQAIRVEGDTLPSSKGVL"
     gene            11295..11885
                     /gene="hisH"
                     /locus_tag="PN977_003928"
     CDS             11295..11885
                     /gene="hisH"
                     /locus_tag="PN977_003928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HDI6043921.1"
                     /translation="MNVVILDTGCANLNSVKSAIARHGYEPKVSRDPDVVLLADKLFL
                     PGVGTAQAAMDQVRERELFDLIKACTQPVLGICLGMQLLGRRSEESNGIDLLGIIDED
                     VPKMTDFGLPLPHMGWNRVYPQAGNRLFQGIEDGAYFYFVHSYAMPVNPWTIAQCNYG
                     EPFTAAVQKDNFYGVQFHPERSGAAGAKLLKNFLEM"
     gene            11885..12622
                     /gene="hisA"
                     /locus_tag="PN977_003929"
     CDS             11885..12622
                     /gene="hisA"
                     /locus_tag="PN977_003929"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HDI6043922.1"
                     /translation="MIIPALDLIDGTVVRLHQGDYGKQRDYGNDPLPRLQDYAAQGAE
                     VLHLVDLTGAKDPAKRQIPLIKTLVAGVNVPVQVGGGVRSEEDVAALLEAGVARVVVG
                     STAVKSPERVKGWFERFGADALVLALDVRIDEQGNKQVAVSGWQENSGVSLEQLVETY
                     LPVGLKHVLCTDISRDGTLAGSNVSLYEEVCARYPQVAFQSSGGIGDINDVAALRGTG
                     VRGVIVGRALLEGKFTVKEAIACWQNA"
     gene            12604..13380
                     /gene="hisF"
                     /locus_tag="PN977_003930"
     CDS             12604..13380
                     /gene="hisF"
                     /locus_tag="PN977_003930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HDI6043923.1"
                     /translation="MLAKRIIPCLDVRDGQVVKGVQFRNHEIIGDIVPLAKRYAEEGA
                     DELVFYDITASSDGRVVDKSWVSRVAEVIDIPFCVAGGIKSLEDAAKILSFGADKISI
                     NSPALADPTLITRLADRFGVQCIVVGIDTWYDAETGKYHVNQYTGDESRTRVTQWETL
                     DWVQEVQKRGAGEIVLNMMNQDGVRNGYDLEQLKKVREVCHVPLIASGGAGTMEHFLE
                     AFRDADVDGALAASVFHKQIINIGELKAYLATQGVEIRIC"
     gene            13374..>13617
                     /locus_tag="PN977_003931"
     CDS             13374..>13617
                     /locus_tag="PN977_003931"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416530.1"
                     /note="catalyzes the formation of 1-(5-phosphoribosyl)-AMP
                     from 1-(5-phosphoribosyl)-ATP and the subsequent formation
                     of
                     1-(5-phosphoribosyl)-5-((5-
                     phosphoribosylamino)methylideneamino)imidazole-4-
                     carboxamide from 1-(5-phosphoribosyl)-AMP in histidine
                     biosynthesis; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase"
                     /protein_id="HDI6043924.1"
                     /translation="MLTEQQRRELDWEKTDGLMPVIVQHAVSGEVLMLGYMNPEALDK
                     TLESGKVTFFSRTKQRLWTKGETSGNFLNVVSIAPDC"
BASE COUNT         3445 a         3628 c         3517 g         3027 t
ORIGIN      
        1 ttacgaaacc tttggaacgc accccgcgtt agatcgccct gcacagttcg cagccattcg
       61 cactgataac gaattcaatg tcatcggcga acccgaagtc ttttactgca agcccgcgga
      121 tgactattta ccccagcctg gagcagtatt aattaccggt attaccccgc aggaagcacg
      181 ggcgaaagga gaaaacgaag ccgcgtttgc cgcccgtatt cactcgcttt ttaccgtacc
      241 gaagacctgt attctgggct acaacaatgt gcgtttcgac gatgaagtca cacgcaacgt
      301 tttttatcgt aatttctacg atccttacgc ctggagctgg cagcatgata actcgcgctg
      361 ggatttactg gatgttatgc gtgcctgtta tgccctgcgc ccggaaggaa taaactggcc
      421 tgaaaatgat gacggtctac cgagctttcg ccttgagcat ttaaccaaag cgaatggtat
      481 tgaacatagc aacgcccacg atgcgatggc tgatgtgtac gccactattg cgatggcgaa
      541 actggtaaaa acgcgtcagc cacgcctgtt tgattatctc tttacccatc gtaataaaca
      601 caaactgatg gcgttgattg atgttccgca gatgaaaccc ctggttcacg tttccggaat
      661 gtttggggca tggcgtggca ataccagctg ggtggcaccg ctggcgtggc atcctgaaaa
      721 tcgcaatgcc gtaattatgg tggatttggc aggagacatt tcaccattac tggagctgga
      781 tagcgacaca ttgcgcgagc gtttatatac cgcaaaagcc gatcttggcg ataacgccgc
      841 cgttccggtt aagctggtgc atatcaataa atgtccggta ctggcccagg cgaatacgct
      901 acgcccggaa gacgctgagc gtttagggat tgctcgtcaa cactgcctgg ataacctgaa
      961 agtgttgcgc gaaaacccgc aagtacgcga caaagttgtg gcaatttttg cagaagctga
     1021 accgtttaca ccatcagata atgtagatgc acagctttat aatggctttt ttagcgacgc
     1081 cgatcgtgca gcaatgaaaa tcgtgctgga gactgaaccg cgtaatttac cggctctgga
     1141 tatcaccttt gttgataagc gtatcgagaa actcctgttc aactaccgcg cccgcaactt
     1201 ccccggcacg ttagatgaca acgagcagca acgctggctt gagcaccgcc gccaggtctt
     1261 cacacccgag tttttacaag gctatgcaga agagattcag atgctggcgc agctgtatgc
     1321 cgacgataaa gaaaaagtgg cgctgttaaa agcgctttgg cagtacgcgg aagagattgt
     1381 ctaatgtgaa gcctccgccg ctggtacggc ggaggacaat tttgagtcag gctttttgaa
     1441 cggtgatgct ccacgctgca tcgccaatct gctgataatc ggtgatggca tgtccttctt
     1501 ctgccgccca ctgtgggatt gcttcagttg cctgggtgca atcaaactca attaccaact
     1561 catcgccgct ggccatttct gccagtgctg cttttgcttc tatgagtgga aacggacaga
     1621 cctgcgtcac cacatccagc tttttaatcg ccatctttac accttactta atttgccgca
     1681 gcagttgcca gtcgcgcctt acgctgcggt cgaacataca caagccagga cgcagtccag
     1741 actccgagaa tcataaatac aaggccaatc cagccctgcc aggtcatcat tgcagtcatg
     1801 accagtccat taccgataga gcaaccacct gcaatgctgg caccgaagcc catcagtaca
     1861 ccacctaacc cgctacgtaa tgttgtttgt gcatcagcag cgcgaacgcg gaactcacgg
     1921 ctcgctttgg cggcaataaa tgaccccacg aagctcccta acaccaggaa aacgccccag
     1981 ttaatgtatt tcacgtcacc cgcgaccaga aattgcagaa tattggccgt tggggaagtg
     2041 atgcctaacc caaacatacg tccggttgct tcactcaggg gccaggctaa aagcgcaatc
     2101 aaaccgatga gtacagctgt gacaaaggga tgccagcgtt tttcaaacag aatatgagcg
     2161 atcccggttc ggcgcggcgg taaggtcgcg actttgagtt ttggcttctt caactctttc
     2221 atcaccaccc agagcgttat caccagcaaa acggcaacca acggccacac agacaaatta
     2281 aaagtatcag caatagagtt atgttcagta gtgtagtgct gcaaggtttg atttaaacca
     2341 ctggcgtgtg gagaacgcat caccgcactc atcaccatat aagtgaaaag cgcgatccaa
     2401 ctgccgatca atccctcacc cgcgcgatac caggtcccgg tggcacatcc acccgccaga
     2461 acaattccca gcccgaaaag atacccacct ataacagtac caagccacgg gaacgccccg
     2521 gcttcgtaag tcagtaggcc cgcctgaatt aacgcaaaaa cccccacgct ttgtaccgaa
     2581 attgcaatca gcaaggcgta aaacatgcga ttatttttcg cgacatacat atcgcgaaaa
     2641 ccacctgtca ggcaaaaacg cccacgctgc atgacaaatc ccagcagagc accacaaatt
     2701 agcccgctta atatcattga aaacataaat taattaacca gatgaatgtt aataaggaaa
     2761 ttattcatga ctggtggaat gcagaccaat aaccaaattc tctaaattag aacaaatggt
     2821 tattaatgag gatttcgata aaaaagaata aaaaaaccgg agccaaatat tcattcgact
     2881 ccggttgatt attagaaaag atgattatgc tacgtcttcg tactgcggaa ccgggttacg
     2941 gaagctcttg gttacgcatg ccaggtaaat caggccgata gcagcccaga tcagacccag
     3001 aaccattgag ctttcttcca ggttaaccca cagcgcacca acggtcagcg caccacacat
     3061 cggcaggaac agatactgga agtgatcttt cagcgtcttg ttacgcttct cacggatcca
     3121 gaactgcgag atgaccgaca ggttaacgaa ggtgaacgcc accaacgcac caaagttaat
     3181 cagcgccgtt gccattacca ggtcaaagtt gattgccagc agggcaatcg cgccaaccag
     3241 gatgatattc atcgccggag tacgccattt cgggtgtaca taaccgaaga agcttttcgg
     3301 gaacacgccg tcacgaccca ttacgtacat cagacgcgca acgcctgcgt gcgccgccat
     3361 accggacgcc agtacggtaa tggtggagaa gatcagcgcg ccaacctgga acgctttacc
     3421 cgcaacgtac agcatgattt caggctgtga cgcatccgga tctttaaagc gagagatatc
     3481 cgggaagtac agctgcagga agtaagttgc aaagatgaag atcatgccgc caatcagcgc
     3541 ggtcaggaaa atcgcacgcg ggatcacgcg ctctgcatct ttggtttctt ccgacaggtt
     3601 gctgatgccg tcaaagccgg taaaggagaa gcacaggatt gtcgccccgg taatcagcgg
     3661 gataacatgc gcatcgccag accagaacgg acgagtgctc gccagcgtac ccgctccttc
     3721 accttcaaat acgccataaa caaccatgcc caggatcacc gcgatcagca ctacctgcaa
     3781 cacgacgatt acagtgttga agttcgctac ggatttcaga ctacgcaggt taaaggcggt
     3841 cataaaggcc accagcgcca ccacaaacat ccacgatgga atggaaggca ccagagcttc
     3901 aaaatagatt ttcgccagca gaatgttgat catcggcgcg aacagatagt cgagcagaga
     3961 agaccaaccc accataaagc cgacagtcgg gctaatggat ttctgggcgt aagtgtatgc
     4021 agagccagca gaaggatagc ggcggaccag cttcccgtag ctcagagccg taaacaggat
     4081 cgcaatcaat gcgaacgcat aggctgtcgg cacatgacca tccgtgaggc ctgaaacgat
     4141 accaaaagta tcaaacagcg tcatcggctg catataggca agacccatca ttacaaccgg
     4201 aactaacgta agcgttttac gcaattccac gcgagaggtg tttggagtaa cgttatgcga
     4261 catggtcatt ctcctttacg gtgataaccg tcgcgtaagc aaaaaattgc cccatttttt
     4321 tggattcctc agcgacaaca actgtcgatt tttagtaaat atctatccgg tacgaagccc
     4381 ggcctcttgg tatgaatgat tggtttgaag caaaaaataa ccgacgctga tgaaacgtcg
     4441 gtttttagtc attttttgac agcggcgcat tgtgcctaaa tgggggggaa atgacaagag
     4501 aatgagaggc ttgtcataat aatttttctt taaatggctg attttccgtc atcagatttt
     4561 gcataaacac cggcgatcgc actatttgct aaaatttcat cccgccacca gccgctggca
     4621 agccctgcgg tctgttcatt ccagccaatc cagacgggtt caaagagagt ttgggcgacc
     4681 actttcttct cgattaacgc gccactatcg agaaaacgtt gcgccagata acggggtaaa
     4741 tagccgcagc cgaggccgct aatttgcagc tccagcttgg ttttaaaatc gaaaacggta
     4801 atggcttctt gttcgtcgag aagttgtgag gccgtagaag cgcctgcctg cgcagtgtcc
     4861 cccaccacaa tcgcccgata tcgcttaatg atacgacggt ttaacggctc ttcttcctgg
     4921 gctaacggat gatgaggcgc gaccgcaaag acttgttcca gatcgcccaa ccgcgagaaa
     4981 ccaaattcac tggaagatgg tggctcatgc atcgcgccaa caataatatc cgctcgcccc
     5041 tgagtcaggg catcccagga accaccgagt acgccattga taaacttcag gcgcgtcacg
     5101 ctatgatgtt gataaaaagc ttcaatcagt ggcgcaagca gagaaaaagg aaacgtgtcg
     5161 tccacgccaa tcaccagctc gttttcccag ccttcatgca gtttgatcgc ctgcttttcc
     5221 agttcccgca cggtatgcag aacttcccgc cctttctcta ataacatttt tccggtgcgg
     5281 gtgaatttag cgcggtgacc gctacgatca agcaattgaa tattaaggtc gctttccagc
     5341 ttgtgaacgg tgtaactcag agcggacggc gtcttgtaga gtttggccga cgccgccgca
     5401 aaactgcctt ctttttctaa ggcatcgagg atcatcaaca catccagcag tggtttcata
     5461 cacgccccct tgcagtgcat gcggcgtcct gctggcgtga tcgtttactc cagcggcatt
     5521 accagcggat cgggatactg gtattcaaac cccagttcat tacaaatccg actgccatca
     5581 ataatcttac ctttgccgct gtccagacta tttctgaact gcggaggttc cagccccagt
     5641 aaacgggcca tctgcggata gaaaacatta cgcgcagggt gagctggcgc acatatatta
     5701 tagatgtgtc cgcctttagg tgcctgtaac aacagagtga tagcgccaat aacatcttct
     5761 aaatggacta aattaacacc atgttcacca tcaggcgcgg tttttccggc aaagaagcgt
     5821 ccgggatgac gtcccggtcc gacaaggccc gcaagacgca gaatatcgac cgaagtaccg
     5881 ggtaaattgt gcagccagtc ttcgagttct tctaatactc ttccactgtt ggttaccgga
     5941 ttacgcgggg tagtttcttt caccgtgcct tgcgcgtcgc catagacaga cgttgagctg
     6001 gtgaaaataa tgcggggaat acgatgagcc agcgcgctat ccactaactc ttgtaccgct
     6061 tgtaaataga actcatcgcc ggggccgcta cgacgtgccg gaagcgtaat gaccagcgca
     6121 tcggcatcca tcagggcatc cagatcgtca gaatcgcaaa ctaactcagg ctccatgcga
     6181 agcagatagc tatcaatgcc actcattcgg gccgcttcga caccatcttg tgtggtttta
     6241 ctcccggtga cttgccagcc tcgcgctgaa agtgacatcg ccagcggcat gcctaaccac
     6301 cctaacccga caattgcgac ctttttcatc cgttatctcc tgatacacct aagatctata
     6361 aggctacgct agcgtatcaa aactgacaat tcattctatg aatgaaatcg aaacggttag
     6421 caaatatacc attgcctgat gcgctccgct tatcagtcct acaaaaacat caatatattg
     6481 aatttacgca acatttgtag accagataag gcattcacgc cgcatccggc aggaacaaag
     6541 cccactttag ttacagaaca caatgttctt gagggtttta aagacatatt ttcacgattt
     6601 cttttctaac gtagtcactc caggccccct cctttccccg ctcattcatt aaacaaatcc
     6661 attgccataa aatatataaa aaagcccttg ctttctaacg tgaaagtggt ttaggttaaa
     6721 agacatcagt tgaataaaca ttcacagaga cttttatgac acgcgttcaa tttaaacacc
     6781 accatcatca ccatcatcct gactagtctt tcaggcgatg tgtgctggaa gacattcaga
     6841 tcttccagtg gtgcatgaac gcatgagaaa gcccccggaa gatcaccttc cgggggcttt
     6901 tttattgcgc ggttgataac ggttcagaca ggtttaaaga ggaataacaa aatgacagac
     6961 aacactcgtt tacgcatagc tatgcagaaa tccggccgtt taagtgatga ttcacgcgaa
     7021 ttgctggcgc gctgtggcat taaaatcaat cttcacaccc agcgcctgat cgcgatggca
     7081 gaaaacatgc cgattgatat tctgcgcgtg cgtgacgacg acattcccgg tctggtaatg
     7141 gatggcgtgg tagaccttgg gattatcggc gaaaacgtgc tggaagaaga gctgcttaac
     7201 cgccgcgccc agggtgaaga tccgcgctac tttaccctgc gtcgtctgga ttttggcggc
     7261 tgccgacttt cactggcaac gccggttgat gaagcctggg acggcccgct ctccttaaac
     7321 ggtaaacgta tcgccacctc ttatcctcac ctgctcaagc gttatctcga ccagaaaggc
     7381 atctctttta aatcctgctt actgaacggt tctgttgaag tcgccccgcg tgccggactg
     7441 gcggatgcga tttgcgatct ggtttccacc ggtgccacgc tggaagcaaa cggcctgcgc
     7501 gaagtcgaag ttatctaccg ctcgaaagct tgcctgatcc aacgcgatgg cgaaatggaa
     7561 gaatccaaac agcaactgat cgacaaactg ctgacccgta ttcagggtgt gatccaggcg
     7621 cgcgaatcaa aatacatcat gatgcacgca ccgaccgaac gtctggatga agtcatcgcc
     7681 ctgctgccag gtgccgaacg cccaactatt ctgccgctgg cgggtgatca acagcgcgta
     7741 gcgatgcaca tggtcagcag cgaaaccctg ttctgggaaa ccatggaaaa actgaaagcg
     7801 ctgggtgcca gttcaatcct ggtcctgccg attgagaaga tgatggagtg atcgccatga
     7861 gctttaacac aatcattgac tggaatagct gtactgcgga gcaacaacgc cagctgttaa
     7921 tgcgcccggc gatctccgct tctgaaagca ttacccgcac tgttaacgat attctcgata
     7981 acgtgaaagc acgcggtgat gacgccctgc gggaatacag cgcgaagttt gataaaacca
     8041 cggttaccgc actgaaggtg tctgctgagg aaattgccgc cgccagcgaa cgcctgagcg
     8101 acgagctaaa acaggcgatg gcggtggcag taaagaatat tgaaaccttc cacactgcgc
     8161 aaaaactgcc gccggtagat gttgaaactc agcctggtgt gcgttgccag caggtcacgc
     8221 gcccggtagc ttcggttggt ttgtatattc ctggcggttc cgccccgctc ttctcaacgg
     8281 tattaatgct ggcgactccg gcgcgtattg cgggttgcaa taaagtggtg ctgtgctcac
     8341 cgccgccgat tgccgatgaa attctttacg cggcgcagct atgcggtgtg caggacgtgt
     8401 ttaacgtcgg cggcgcacag gccattgccg cgctggcgtt tggtacggaa tctgtgccga
     8461 aagtggacaa aatcttcggg ccgggtaacg cctttgtcac cgaagcgaaa cgtcaggtga
     8521 gccagcgtct ggacggtgcg gcgatcgata tgcccgcagg cccgtcggaa gtgctggtga
     8581 ttgctgacag cggcgctacg ccggatttcg tggcttctga tttgctttct caggctgaac
     8641 acggcccgga ttcacaggtg attttactga cgcctgacgc tgatatggcg catcaagttg
     8701 ccgaagccgt cgaacgccag ttagcagaac tgccgcgtgc cgaaaccgca cgtcaggcac
     8761 tgagcgccag ccgcctgatc gtgaccaacg atttagcgca gtgcgtggca atctccaacc
     8821 agtacggccc ggagcacctg atcattcaga cccgcaacgc ccgcgaactg gtcgatagca
     8881 tcaccagcgc cggttcggta tttcttggtg actggtcacc ggaatcggca ggtgattacg
     8941 cctccggcac caaccacgtt ctgccgactt acggttacac cgccacctgt tccagcctcg
     9001 gactggcgga tttccagaag cggatgaccg tgcaggaact gtcgaaagta ggtttctccg
     9061 cgctggcttc gaccattgaa acactggccg ccgccgagcg cctgaccgcc cacaaaaatg
     9121 ccgttacttt gcgtgttaac gcccttaagg agcaagcatg agcaccgtga ctattaccga
     9181 tttagcgcgt gagaacgtcc gcaacctgac gccgtatcag tcggcgcgtc gtctgggcgg
     9241 taacggcgac gtctggctga acgccaacga ataccccaca gccgtggagt ttcagcttac
     9301 tcagcaaacg ctcaaccgct acccggaatg ccagccgaaa gcggtgattg aaaattacgc
     9361 gcagtatgca ggcgtgaagg cggagcaggt gctggtcagc cgtggcgcgg acgaaggtat
     9421 tgaactgctg attcgcgctt tttgcgaacc gggtaaagac gccatcctct actgcccgcc
     9481 aacgtacggc atgtacagcg tcagcgccga aaccattggc gtcgagtgcc gcacagtgcc
     9541 gacgctgaaa aactggcaac tggacttgca gggcatttcc gacaagctgg acggcgtaaa
     9601 agtggtttat gtttgcagcc ccaacaaccc gaccgggcaa ctgatcaatc cacaggattt
     9661 tcgcaccctg ctggagttaa cgcgcggtaa agcgattgtg gttgccgatg aggcctatat
     9721 cgagttttgc ccgcaggcat cgctggctgg ctggctggcg gaatatccgc acctggctat
     9781 tttgcgcaca ctgtcgaaag ccttcgctct ggcgggcctt cgttgcggat ttacgctggc
     9841 aaacgaagaa gtcatcaacc tgctgatgaa agtgatcgcc ccctacccgc tctcgacgcc
     9901 ggttgccgac attgcagccc aggcgttaag cccgcagggg atcgtcgcca tgcgcgaacg
     9961 agtggcgcaa attattgctg aacgcgaata cctgatggcc gcactgaaag agatcccctg
    10021 cgtggagcag gttttcgact ccgaaaccaa ctacattctg gcgcgcttta aagcctccag
    10081 cgcagtgttt aaatctttgt gggatcaggg cattatctta cgtgatcaga ataaacaacc
    10141 ctctttaagc ggctgcctgc gaattaccgt cggaacccgt gaagaaagcc agcgcgtcat
    10201 tgacgcctta cgtgcggagc aagtttgatg agtcagaagt atctttttat cgatcgcgat
    10261 ggaaccctga ttagcgaacc gccgagtgat tttcaggtgg accgttttga caaactcgcc
    10321 tttgaaccgg gcgtgatccc ggaactgctg aagctgcaaa aagcgggcta caaactggtg
    10381 atgatcacta atcaggatgg tctgggaaca caaagtttcc cgcaggcgga ttttgatgga
    10441 ccgcacaacc tgatgatgca gatcttcacc tcgcaaggcg tgcagtttga tgaagtgctg
    10501 atttgtccgc acctgcccgc agatgagtgc gactgccgta aaccgaaagt aaaactggtg
    10561 gagcgttatc tcgctgagca agcgatggat cgtgccaaca gttatgtgat tggcgatcgc
    10621 gcgaccgata ttcaactggc ggaaaacatg ggtattaatg gtttacgcta cgaccgcgaa
    10681 accctgaact ggccgatgat tggcgagcaa ctcactaaac gagaccgtta cgcccacgta
    10741 gtgcgcaaca ccaaagagac gcaaattgac gtccaggtgt ggctggatcg tgaaggtggc
    10801 agcaagatta acaccggcgt tggcttcttt gatcacatgc tggatcagat cgccacccac
    10861 ggcggtttcc gcatggaaat caacgtcaaa ggcgacctct atatcgacga tcaccacacc
    10921 gtcgaagata ccggcctggc gctgggcgaa gcgttaaaaa ttgccctcgg cgataaacgc
    10981 ggtatttgtc gctttggttt tgtgctgccg atggacgaat gccttgcccg ctgcgcgctg
    11041 gatatctctg gtcgcccgca cctggaatat aaagccgaat ttacctacca gcgcgtgggc
    11101 gatctcagca ctgaaatgat cgagcacttc ttccgttcgc tctcttacac tatgggcgtg
    11161 acgctacacc tgaaaaccaa aggtaaaaac gatcatcacc gtgtagagag cctgttcaaa
    11221 gcctttggtc gcaccctgcg ccaggccatc cgcgtggaag gcgacaccct gccctcgtca
    11281 aaaggagtgc tgtaatgaac gtggtgatcc ttgataccgg ctgcgccaat ctgaactcgg
    11341 tgaagtctgc cattgcgcgc cacggttatg aacccaaagt cagccgtgac ccggacgtcg
    11401 tgttgctggc cgataaactg tttttacccg gcgttggcac cgcgcaagcg gcgatggacc
    11461 aggtgcgtga gcgcgagctg tttgatctca tcaaagcctg tacccaaccg gtgctgggca
    11521 tctgcttagg gatgcaactg ctggggcggc gcagcgaaga gagcaacggc atcgacttgc
    11581 tgggcatcat cgacgaagac gtgccgaaaa tgaccgactt tggtctgccg ctgccgcata
    11641 tgggctggaa ccgcgtttac ccgcaggcag gcaaccgcct gtttcagggg attgaagacg
    11701 gcgcgtactt ttactttgtt cacagctacg caatgccagt caatccgtgg accatcgccc
    11761 agtgtaacta cggcgaaccg ttcaccgcgg cggtacaaaa agataacttc tatggcgtgc
    11821 agttccaccc ggagcgttct ggtgccgctg gcgctaagtt gctgaaaaac ttcctggaga
    11881 tgtgatgatt attccggcat tagatttaat cgacggcacc gtggtgcgtc tccatcaggg
    11941 cgattatggc aaacaacgcg attacggtaa cgacccgctg ccacgcttac aggattacgc
    12001 cgcacaaggt gctgaagtgc tgcacctggt ggatctgacc ggggcaaaag atccggctaa
    12061 acgtcaaatc ccgctgatta aaaccctggt cgcgggcgtt aacgttccgg tgcaggttgg
    12121 cggcggcgtg cgtagcgaag aagacgtagc ggcgttactg gaagcaggtg ttgcgcgtgt
    12181 ggtagttggc tccaccgcgg tgaaatcacc agaaagggtg aaaggctggt ttgaacgctt
    12241 cggtgcggat gccttagtgc tggcgctgga tgtccgtatt gacgagcaag gcaacaagca
    12301 agtggcggtc agtggctggc aagagaattc gggtgtgtcg ctggaacaac tggtggaaac
    12361 ctatctgccc gtcggcctga aacatgtgct gtgtaccgat atctcgcgcg acggcacgct
    12421 ggcaggctct aacgtctctt tatatgaaga agtgtgcgcc agatatccgc aagtagcatt
    12481 tcagtcctcc ggcggtattg gcgacattaa tgatgtcgct gccctgcgtg gcactggcgt
    12541 gcgcggcgta atagttggtc gggcattact ggaaggtaaa ttcaccgtga aggaggccat
    12601 cgcatgctgg caaaacgcat aattccgtgt ctcgacgttc gtgatggtca ggtggtgaaa
    12661 ggcgtacagt ttcgcaacca tgaaatcatt ggcgatatcg tgccgctagc aaaacgctac
    12721 gccgaagaag gtgcggacga gctggtgttc tacgatatca ccgcttccag cgatggacgg
    12781 gtggtagata aaagctgggt atctcgcgtg gcggaagtga tcgacattcc gttttgtgtg
    12841 gcgggtggga ttaagtctct ggaagatgcc gcgaaaattc tttcctttgg cgcggataaa
    12901 atttccatca actctcctgc gctggcggac ccgacgttaa ttactcgcct ggccgatcgc
    12961 tttggcgtgc agtgtattgt ggtcggtatt gatacctggt acgacgccga aaccggtaaa
    13021 tatcatgtga atcaatatac cggcgatgaa agccgcaccc gcgtcactca atgggaaacg
    13081 ctcgactggg tacaggaagt gcaaaaacgc ggtgccggag aaatcgtcct caatatgatg
    13141 aatcaggacg gcgtgcgtaa cggttacgac ctcgaacaac tgaaaaaagt gcgtgaagtt
    13201 tgccacgtcc cgctgattgc ctccggtggc gcgggcacca tggaacactt cctcgaagcc
    13261 ttccgcgatg ccgacgttga cggcgcgctg gcagcttccg tattccacaa acaaataatc
    13321 aatattggtg aattaaaagc gtacctggca acacagggcg tggagatcag gatatgttaa
    13381 cagaacaaca acgtcgcgaa ctggactggg aaaaaaccga cggacttatg ccggtgattg
    13441 tgcaacacgc ggtatccggc gaagtgctaa tgctgggcta tatgaacccg gaagccttag
    13501 acaaaaccct cgaaagcggc aaagtcacct tcttctcgcg cactaaacag cgactgtgga
    13561 ccaaaggcga aacgtcgggc aatttcctca acgtagtgag tattgccccg gactgcg
//
LOCUS       DAMZFT010000127        13441 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.127, whole genome shotgun sequence.
ACCESSION   DAMZFT010000127 DAMZFT010000000
VERSION     DAMZFT010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13441)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13441)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13441
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.127"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(74..958)
                     /gene="xapR"
                     /locus_tag="PN977_003932"
     CDS             complement(74..958)
                     /gene="xapR"
                     /locus_tag="PN977_003932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator XapR"
                     /protein_id="HDI6043925.1"
                     /translation="MERVYRTDLKLLRYFLAVAEELHFGRAAARLNMSQPPLSIHIKE
                     LENQLGTQLFIRHSRSVVLTHAGKILMEESRRLLVNANNVLARVEQIGRGEAGRIELG
                     VVGTAMWGRMRPVMRRFLRENPNVEVLFREKMPAMQMALLERRELDAGIWRMATEPPT
                     GFTSLRLHESAFLVAMPEEHHLSSFSTVPLEALRDEYFVTMPPVYTDWDFLQRVCQQV
                     GFSPVVIREVNEPQTVLAMVSMGIGITLIADSYAQMNWPGVIFRPLKERIPADLYIVY
                     ETQQVTPALVKLLAALAQ"
     gene            complement(1000..1158)
                     /locus_tag="PN977_003933"
     CDS             complement(1000..1158)
                     /locus_tag="PN977_003933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001392449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1427 family protein"
                     /protein_id="HDI6043926.1"
                     /translation="MKDILFSSGVGFGIGALFTIVRLPIPVPNVLPGILSIVFMYVGY
                     LVVKYFMP"
     gene            complement(1211..2467)
                     /gene="xapB"
                     /locus_tag="PN977_003934"
     CDS             complement(1211..2467)
                     /gene="xapB"
                     /locus_tag="PN977_003934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthosine/proton symporter XapB"
                     /protein_id="HDI6043927.1"
                     /translation="MSIALRLKVMSFLQYFIWGSWLVTLGSYMINTLHFTGANVGMVY
                     SSKGIAAIIMPGIMGIIADKWLRAERAYMLCHLVCAGVLFYAASVTDPDMMFWVMLVN
                     AMAFMPTIALSNSVSYSCLAQAGLDPVTAFPPIRVFGTVGFIVAMWAVSLLHLELSSL
                     QLYIASGASLLLSAYALTLPKIPVAEKKATTSLASKLGLDAFVLFKNPRMAIFFLFAM
                     MLGAVLQITNVFGNPFLHDFARNPEFADSFVVKYPSILLSVSQMAEVGFILTIPFFLK
                     RFGIKTVMLMSMVAWTLRFGFFAYGDPSPTGFILLLLSMIVYGCAFDFFNISGSVFVE
                     QEVDSSIRASAQGLFMTMVNGVGAWVGSILSGMAVDYFSVDGVKDWHTIWLMFAGYAL
                     FLAVIFFFGFKYNHDPEKITHRSVTH"
     gene            complement(2528..3361)
                     /gene="xapA"
                     /locus_tag="PN977_003935"
     CDS             complement(2528..3361)
                     /gene="xapA"
                     /locus_tag="PN977_003935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthosine phosphorylase"
                     /protein_id="HDI6043928.1"
                     /translation="MYQAQFSHNPLYCVDIIKTYKPDFTPRVAFILGSGLGALADQIE
                     NAVAISYEKLPGFPVSTVHGHAGELVLGYLQGVPVACMKGRGHFYEGRGMTIMTDAIR
                     TFKLLGCELLFCTNAAGSLRPEVGAGSLVALKDHINTMPGTPMVGLNDERFGERFFSL
                     ANTYDAEYRALLQKVAKEEGFPLTEGVFVSYPGPNFETAAEIRMMQIIGGDVVGMSVV
                     PEVISARHCELKVVAVSAITNMAEGLSDVKLSHAQTLAAAELSKQNFINLICGFLRKI
                     A"
     gene            3610..4374
                     /locus_tag="PN977_003936"
     CDS             3610..4374
                     /locus_tag="PN977_003936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6043929.1"
                     /translation="MKKHLFTLTLSSVLAIPAVSHAEFKGGFADIGIHYLDWTSRTTE
                     KSSTKSHKDDFGYLELEGGANFSWGEMYGFFDWENFYNNRHDKPGSEQRYTFKNTNRI
                     YLGDTGFNLYLHAYGTYGSANRVNFHDDMFLYGIGYNFTGSGWWFKPFFAKRYTDQTY
                     YTGVNGYVAGWVAGYSFMLGSEKFTLTNWNEYEFDRDATYAASNGGKEGLNGAVALWW
                     NATSHITTGIQYRYADDKLGEDFYQDAIIYSIKFNF"
     gene            complement(4412..5338)
                     /locus_tag="PN977_003937"
     CDS             complement(4412..5338)
                     /locus_tag="PN977_003937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDI6043930.1"
                     /translation="MNYSLKQLKVFVTVAQEKSFSRAGERIGLSQSAVSHSVKELENH
                     TGVRLLDRTTREVVLTDAGQQLALRLERLLDELNSTLRDTGRMGQQLSGKVRVAASQT
                     ISAHLIPQCIAESHRRYPDIQFVLHDRPQQWVMESIRQGDVDFGIVIDPGPVSDLQCE
                     AILSEPFFLLCHRDSALAAEDYVPWQALQGAKLVLQDYASGSRPLIDAALARNGIQAN
                     IVQEIGHPATLFPMVAAGIGISILPALALPLPEGSPLVVKRITPVVERQLMLVRRKNR
                     SLSTAAQALWDVVRGQGRALMAGREGDPLYQI"
     gene            5428..6426
                     /locus_tag="PN977_003938"
     CDS             5428..6426
                     /locus_tag="PN977_003938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter"
                     /protein_id="HDI6043931.1"
                     /translation="MKLFRILDPFTLTLITVVLLASFFPARGDSVPFFENLTTAAIAL
                     LFFMHGAKLSREAIIAGGGHWRLHLWVMCSTFVLFPILGVLFAWWKPVNVAPMLYSGF
                     LYLCILPATVQSAIAFTSMAGGNVAAAVCSASASSLLGIFLSPLLVGLVMNVHGAGGS
                     LEQVGKIMLQLLLPFVLGHLSRPWIGDWVSRNKKWIAKTDQTSILLVVYTAFSEAVVN
                     GIWHKVGWGSLLFIVVVSCVLLAIVIVVNVFMARRLGFNKADEITIVFCGSKKSLANG
                     IPMANILFPTSVIGMMVLPLMIFHQIQLMVCAVLARRYKRQTEQLQAQQESSADKA"
     gene            complement(6423..6641)
                     /locus_tag="PN977_003939"
     CDS             complement(6423..6641)
                     /locus_tag="PN977_003939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3820 family protein"
                     /protein_id="HDI6043932.1"
                     /translation="MEKEQLIEIANTIMPFGKYKGRRLIDLPEEYLLWFARKDEFPAG
                     KLGELMQITLLIKTEGLTQLVQPLKRTL"
     gene            complement(6643..8658)
                     /gene="ligA"
                     /locus_tag="PN977_003940"
     CDS             complement(6643..8658)
                     /gene="ligA"
                     /locus_tag="PN977_003940"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HDI6043933.1"
                     /translation="MESIEQQLTELRTTLRHHEYLYHVMDAPEIPDAEYDRLMRELRE
                     LETKHPELITPDSPTQRVGAAPLAAFSQIRHEVPMLSLDNVFDEESFLAFNKRVQDRL
                     KSNEKVTWCCELKLDGLAVSILYENGVLVSAATRGDGTTGEDITSNVRTIRAIPLKLH
                     GENIPARLEVRGEVFLPQAGFEKINEDARRTGGKVFANPRNAAAGSLRQLDPRITAKR
                     PLTFFCYGVGVLEGGELPDTHLGRLLQFKKWGLPVSDRVTLCESAEEVLAFYHKVEED
                     RPTLGFDIDGVVIKVNSLAQQEQLGFVARAPRWAVAFKFPAQEQMTFVRDVEFQVGRT
                     GAITPVARLEPVHVAGVLVSNATLHNADEIERLGLRIGDKVVIRRAGDVIPQVVNVVL
                     SERPEDTREVVFPTHCPVCGSDVERVEGEAVARCTGGLICGAQRKESLKHFVSRRAMD
                     VDGMGDKIIDQLVEKEYVHTPADLFKLTAGKLTGLERMGPKSAQNVVNALEKAKETTF
                     ARFLYALGIREVGEATAAGLAAYFGTLEALEAASIEELQKVPDVGIVVASHVHNFFAE
                     ESNRNVISELLAEGVHWPEPIVINAEEIDSPFAGKTVVLTGSLSQMSRDDAKARLVEL
                     GAKVAGSVSKKTDLVIAGEAAGSKLAKAQELGIEVIDETEMLRLLGS"
     gene            complement(8729..9727)
                     /gene="zipA"
                     /locus_tag="PN977_003941"
     CDS             complement(8729..9727)
                     /gene="zipA"
                     /locus_tag="PN977_003941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZipA"
                     /protein_id="HDI6043934.1"
                     /translation="MMQDLRLILIIVGAIAIIALLVHGFWTSRKERSSMFRDRPLKRM
                     KSKRDDDSYDEDVEDDEGVGEVRVHRVNHAPANAQEHEAARPSPQHQYQPPYASAQPR
                     QPVQQPPEAQVPPQHAPRPAQPVQQPVQQPAYQPQPEQPLQQPVSPQVASAPQPVHSA
                     PQPAQQAFQPAEPVAAPQPEPVAEPAPVMDKPKRKEAVIIMNVAAHHGSELNGELLLN
                     SIQQAGFIFGDMNIYHRHLSPDGSGPALFSLANMVKPGTFDPEMKDFTTPGVTIFMQV
                     PSYGDELQNFKLMLQSAQHIADEVGGVVLDDQRRMMTPQKLREYQDIIREVKDANA"
     gene            9957..10718
                     /gene="cysZ"
                     /locus_tag="PN977_003942"
     CDS             9957..10718
                     /gene="cysZ"
                     /locus_tag="PN977_003942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate transporter CysZ"
                     /protein_id="HDI6043935.1"
                     /translation="MVSSFTSAPRSGFYYFAQGWKLVSQPGIRRFVILPLLVNILLMG
                     GAFWWLFTQLDVWIPTLMSYVPDWLQWLSYLLWPLAVISVLLVFGYFFSTIANWIAAP
                     FNGLLAEQLEARLTGATPPDTGIFGIMKDVPRIMKREWQKFAWYLPRAIVLLILYFIP
                     GIGQTVAPVLWFLFSAWMLAIQYCDYPFDNHKVPFKEMRTALRTRKITNMQFGALTSL
                     FTMIPLLNLFIMPVAVCGATAMWVDCYRDKHAMWR"
     gene            10903..11874
                     /gene="cysK"
                     /locus_tag="PN977_003943"
     CDS             10903..11874
                     /gene="cysK"
                     /locus_tag="PN977_003943"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HDI6043936.1"
                     /translation="MSKIFEDNSLTIGHTPLVRLNRIGNGRILAKVESRNPSFSVKCR
                     IGANMIWDAEKRGVLKPGVELVEPTSGNTGIALAYVAAARGYKLTLTMPETMSIERRK
                     LLKALGANLVLTEGAKGMKGAIQKAEEIVASNPEKYLLLQQFSNPANPEIHEKTTGPE
                     IWEDTDGQVDVFIAGVGTGGTLTGVSRYIKGTKGKTDLISVAVEPTDSPVIAQALAGE
                     EIKPGPHKIQGIGAGFIPANLDLKLVDKVIGITNEEAISTARRLMEEEGILAGISSGA
                     AVAAALKLQEDESFTNKNIVVILPSSGERYLSTALFADLFTEKELQQ"
     gene            12258..12515
                     /gene="ptsH"
                     /locus_tag="PN977_003944"
     CDS             12258..12515
                     /gene="ptsH"
                     /locus_tag="PN977_003944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003038086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein Hpr"
                     /protein_id="HDI6043937.1"
                     /translation="MFQQEVTITAPNGLHTRPAAQFVKEAKGFTSEITVTSNGKSASA
                     KSLFKLQTLGLTQGTVVTISAEGEDEQKAVEHLVKLMAELE"
     gene            12560..>13441
                     /gene="ptsP"
                     /locus_tag="PN977_003945"
     CDS             12560..>13441
                     /gene="ptsP"
                     /locus_tag="PN977_003945"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HDI6043938.1"
                     /translation="MISGILASPGIAFGKALLLKEDEIVIDRKKISADQVDQEVERFL
                     SGRAKASAQLETIKTKAGETFGEEKEAIFEGHIMLLEDEELEQEIIALIKDKHMTADA
                     AAHEVIEGQASALEELDDEYLKERAADVRDIGKRLLRNILGLKIIDLSAIQDEVILVA
                     ADLTPSETAQLNLKKVLGFITDAGGRTSHTSIMARSLELPAIVGTGSVTSQVKNDDYL
                     ILDAVNNQVYVNPTNEVIDKMRAVQEQVASEKAELAKLKDLPAITLDGHQVEVCANIG
                     TVRDVEGAERNGAEGVGL"
BASE COUNT         3401 a         3519 c         3199 g         3322 t
ORIGIN      
        1 tgcaatttgc gaaattgcct gatgcgcttc gcttatcaga ccaactatgt ccggcagttc
       61 ccgtgcctct catctactgc gccaacgccg ccagcagttt aaccagcgcg ggcgtcacct
      121 gctgcgtttc ataaacaata tataagtctg ccgggatgcg ttctttgagc ggacggaata
      181 tgacaccggg ccagttcatc tgcgcgtagc tgtccgctat caatgtgata ccaataccca
      241 tactgaccat ggcgagtacc gtttgcggtt cattaacttc gcgaataaca accggtgaaa
      301 atcccacctg ctggcaaact cgctgcaaaa aatcccagtc agtgtacacg ggcggcattg
      361 taacgaaata ctcgtcacgt agcgcttcca gcgggacggt ggaaaatgat gagagatgat
      421 gctcttcagg catcgccacc agaaacgccg attcatgcaa ccgtaagctg gtaaaaccag
      481 tcggtggttc tgtcgccatt cgccagatcc cggcatcaag ttcgcggcgt tccagcaagg
      541 ccatttgcat cgcgggcatc ttttcgcgaa acagaacttc aacgttagga ttttctctaa
      601 ggaatcgccg cataaccggg cgcatccgtc cccacattgc cgttcccaca accccgagtt
      661 caatccgccc tgcttctccc cgacctattt gttcaacccg agccaataca ttatttgcat
      721 tcaccagcaa tcgacgcgat tcttccatca agattttgcc cgcgtgtgtc agtacgacgc
      781 tgcgcgaatg gcgaataaaa agctgcgtgc cgagttgatt ttccagctct ttaatatgaa
      841 tactgagcgg aggctgagac atatttaaac gcgctgctgc gcgaccaaaa tgcaactctt
      901 ccgctacggc aagaaaataa cggagcaact taagatctgt tctatatacg cgttccataa
      961 ttaagtgctc catataaaaa aatatatcgt attaaattat catggcataa agtatttcac
     1021 caccagatat ccgacataca taaacacgat ggataatata cccggcaata catttggaac
     1081 aggaataggt aatcgcacga ttgtaaacaa agcaccaatg ccaaaaccaa cgccagaaga
     1141 aaataaaata tctttcacta attaaccttt atcataaaag cagccccaag gaacagagct
     1201 gcgaatcctt ttaatgtgta actgaacgat gcgttatctt ttcagggtca tggttatatt
     1261 taaacccaaa gaaaaatatc actgcgagga aaagagcata ccctgcaaac atcagccaaa
     1321 tggtatgcca atcttttacg ccatccaccg agaagtaatc cactgccata ccgctcagaa
     1381 tcgagccaac ccatgcgccg acgccattta ccatcgtcat aaaaaggccc tgggcgctgg
     1441 cacgaatgct ggaatcaact tcctgttcga caaagaccga accagaaata ttgaagaaat
     1501 cgaatgcaca accgtaaaca atcatcgaca gcaacagcaa aataaacccg gttggtgatg
     1561 ggtcgccata ggcaaagaag ccaaagcgca gcgtccacgc caccatactc atcagcatga
     1621 cggttttaat accgaaacgc ttcaaaaaga acggtatcgt cagaatgaaa ccgacttcag
     1681 ccatctgtga aactgacagt aaaatggacg ggtatttcac cacgaagcta tcagcaaact
     1741 caggattacg ggcgaaatca tgcaggaacg ggttgccaaa aacgttggta atttgcagca
     1801 ccgcacccag catcatggcg aagaggaaaa agatagccat gcgtggattt ttaaacagca
     1861 cgaaggcatc caggcccagc ttgctggcaa gcgatgtagt cgcttttttc tccgcaaccg
     1921 gaatcttcgg caaagtcagc gcataagccg acagcagcaa tgacgcaccg gacgcgatat
     1981 acagttgcag actactcaat tccagatgca gcaggcttac tgcccacatg gcgacaatga
     2041 accccacagt accaaaaacg cgaatgggcg ggaaagcggt caccgggtca agccctgcct
     2101 gggcaagaca ggaataagag acgctgttcg ataacgcaat agtcggcata aacgccatcg
     2161 cattgactaa catcacccaa aacatcatat ccggatcagt tacggatgcc gcataaaaaa
     2221 gtacgcctgc acacaccagg tgacacagca tgtatgcacg ttctgcacgc agccatttgt
     2281 ctgcgatgat ccccattata ccaggcataa taatcgcggc gatccctttg gaactgtaaa
     2341 ccatgccaac attagcgccg gtgaaatgaa gggtattaat catgtaagaa ccgagggtaa
     2401 ccagccagct cccccagata aaatattgca aaaaggacat tacctttaag cgcaaagcga
     2461 tgctcataat cgtttccttg tcgcatcata tggcctcact cagtgaggcc gtttattgtt
     2521 gttttactca ggcaattttg cgcagaaagc cgcaaataag attaataaag ttttgctttg
     2581 agagttccgc tgctgccagc gtttgggcat gagaaagctt cacgtcactc agaccttccg
     2641 ccatgttggt aatcgcagag accgcaacga ctttaagttc gcaatggcga gcagaaataa
     2701 cctcaggcac cacagacata ccaacaacat ccccaccaat aatttgcatc atgcgaattt
     2761 ccgccgcagt ctcgaaattc ggacccggat acgagacgaa cacgccctcc gtcagaggga
     2821 acccctcttc tttcgccact ttttgtaaca gtgcgcggta ttccgcatcg taggtatttg
     2881 ccagcgagaa gaagcgctca ccaaaacgtt catcattaag acccaccatc ggcgttcccg
     2941 gcatggtgtt gatgtgatct ttcaatgcga ccagactgcc ggcccccact tcaggacgca
     3001 gtgagccagc cgcattggtg cagaacagca actcgcagcc cagcaactta aaggtacgga
     3061 ttgcatccgt catgatggtc atcccacgac cttcgtagaa atgtccgcga cctttcatgc
     3121 acgccactgg caccccctgg agataaccca gcaccagctc acccgcatga ccgtgtacgg
     3181 tacttaccgg gaacccaggc agcttttcgt aggaaattgc gaccgcgttc tcaatctgat
     3241 cggccagcgc gcccagcccg gaacctaaaa taaaggccac tcgtggcgtg aaatcaggtt
     3301 tataagtctt gataatatct acgcaataca gtgggttatg agaaaactga gcctgataca
     3361 tatatatcct ttttttgtag ggtggaatct aacgcctttt tataacgaga ttgccctaac
     3421 tcataccaat acactttttt catgtatccg atagttattc gatatcgata tatttatatt
     3481 ttttcactat taatagactg aaaaaactgt attggtactc tcctttccct tgcattaaca
     3541 atatgccgct tttaatcaac ctttttatta agcaatcaac taataacgat cagcataggg
     3601 aattatgcta tgaaaaaaca tctttttact ctgacactct cttctgtatt ggcgatacca
     3661 gcagtatccc atgcagaatt taaaggcggt tttgctgata taggtattca ttatcttgac
     3721 tggactagca gaaccactga gaaatcgtcg accaaatcac acaaagatga ttttggatat
     3781 ctggaactgg aaggcggcgc taactttagt tggggagaaa tgtacgggtt cttcgactgg
     3841 gaaaactttt ataataatcg tcatgataaa ccgggtagcg agcagcgtta cacatttaaa
     3901 aataccaacc gtatttacct gggtgacacc ggatttaatc tctacttgca tgcgtatggc
     3961 acctacggtt ctgcgaatcg tgtgaatttc catgacgata tgttccttta cgggatcggc
     4021 tacaatttca ccggcagcgg ttggtggttc aaaccgttct ttgcaaaacg ctatacagac
     4081 caaacctatt ataccggcgt taacggttat gtcgccggtt gggttgcagg ttacagcttt
     4141 atgctgggca gtgagaaatt caccctcacc aactggaacg agtacgagtt tgatcgtgat
     4201 gccacctacg cggcaagtaa tggcggcaaa gaaggtctga acggtgccgt cgcactttgg
     4261 tggaatgcaa cttcacacat caccacaggg attcagtatc gttatgcgga tgacaaactg
     4321 ggcgaagatt tttaccagga tgcaataatc tactccatca aatttaattt ctgattgatt
     4381 actgccgggg ctcccggcag tatgaatagc tctatatctg atacagcgga tccccttccc
     4441 gcccagccat tagcgcacgg ccctggcctc gtaccacgtc ccataacgct tgtgcggcgg
     4501 tggaaagtga acggttcttc ctgcgcacca gcatcaactg gcgttccacc accggcgtaa
     4561 tgcgctttac caccagcggg ctaccttcag gcaatggtag cgccagtgcg ggaagaatac
     4621 tgatgccgat gcctgcggct accatcggga acagcgtcgc cggatgtcca atctcctgca
     4681 caatattcgc ctgaatacca ttgcgcgcca gagctgcgtc aatcagcggt cggctgcctg
     4741 acgcgtaatc ctgcaacacc aatttcgctc cctgtaatgc ctgccagggg acataatctt
     4801 ccgcggccaa agcgctatcg cgatggcaaa gcaagaagaa aggctcggaa agaatcgctt
     4861 cacattgcag atcgctcact gggcctggat cgatgacgat gccaaaatcg acatctccct
     4921 ggcgaatact ttccatcacc cactgctgcg gacgatcgtg caggacaaac tgaatatctg
     4981 gatagcggcg atggctttca gcaatgcatt gcggaataag atgcgcggaa atggtctggc
     5041 tggcagcgac ccgaactttt ccgctcagtt gttgccccat acgcccggta tcgcgcaacg
     5101 tgctgttaag ttcatccagc agtcgctcaa gacgcaaagc caactgctgc cctgcatctg
     5161 taagcaccac ttcacgcgtg gttctgtcca gcaggcgaac accagtatga ttttccagct
     5221 ccttcacact gtgactcact gccgactggc tcaggccgat acgctctcct gcacgactaa
     5281 aacttttctc ctgcgctact gtgacgaaaa cctttaattg ttttaaagaa taattcatct
     5341 gttttcttca tgaatggatg caataaatca attttatttc tcaaactgga agaagcacaa
     5401 tagaaccatc gatcatctgg agtctttatg aaactttttc gtatcctcga tcctttcacc
     5461 ttaaccctga tcacggtggt gctgctggcc tctttctttc cggccagagg cgattccgtt
     5521 cccttctttg aaaatctgac taccgcagcc attgctctgc tgttctttat gcacggcgcg
     5581 aagttatcgc gagaagccat tattgcaggc ggtggtcatt ggcgactgca tttatgggtg
     5641 atgtgtagca ccttcgtgct gttcccaatt ttgggcgtgc tgtttgcctg gtggaaaccg
     5701 gtaaatgtcg ccccgatgct ctattccggt tttctctact tgtgcattct tcctgctacc
     5761 gtgcagtctg caatcgcctt cacgtcaatg gcgggcggta acgtcgcggc ggcggtttgt
     5821 tctgcgtcag catccagcct gctggggatt ttcctttcac cattgctggt tggtctggtg
     5881 atgaatgttc acggtgcagg gggcagcctt gagcaggtcg gtaaaattat gctgcaactg
     5941 ctgctgccgt ttgtgttggg gcatctttcc cggccgtgga ttggtgactg ggtgtcgcgc
     6001 aataaaaaat ggattgcgaa aactgaccag acgtccattc tgttggtggt ttatacggca
     6061 ttcagcgaag ccgtcgttaa cggtatctgg cacaaagttg gctggggatc attgctgttt
     6121 atcgtggtgg tcagttgcgt tcttctggct atcgtgattg tagttaacgt ctttatggca
     6181 cgccgactgg gcttcaataa ggcagatgaa attactatcg tcttttgtgg ttcgaaaaag
     6241 agtctggcaa atggcatccc gatggcaaac attctgttcc ccacatcggt gatcggtatg
     6301 atggtgctgc ctctgatgat tttccatcag atccaattga tggtctgtgc ggtgctggcg
     6361 cgtcgataca aacgccagac cgaacagtta caggcgcagc aggaaagcag cgccgataaa
     6421 gcttaaagtg tgcgtttcag gggctggacc agttgcgtca gcccctcggt tttaatcagc
     6481 agcgtgattt gcattagctc accgagcttc cctgccggga actcatcttt gcgggcaaac
     6541 cacagcagat actcctccgg caggtcgatt aagcgacgcc ctttgtactt gccaaacggc
     6601 attatcgtat tggctatttc aatcagctgc tctttttcca tctcagctac ccagcaaacg
     6661 cagcatttcc gtttcgtcga tgacttcaat gcccagttcc tgcgccttcg ccagtttaga
     6721 tcctgcagct tcacccgcta tcaccagatc ggttttcttc gacacgctgc ccgcgacttt
     6781 cgcccccagt tcgaccagtc gagctttagc gtcatcacgc gacatctggc ttaagctgcc
     6841 cgtaagcacc acggttttac cagcaaacgg actgtcaatc tcttccgcat tgataacgat
     6901 cggctcaggc cagtgaacac cttccgccaa cagctcgctg atgacattgc ggttgctttc
     6961 ttcggcaaag aagttgtgaa cgtgggaggc aacgacaata ccaacatcag gtaccttttg
     7021 cagctcttca atcgaagcgg cttccagcgc ttccagcgtg ccgaaatatg ccgccagacc
     7081 tgctgcggtg gcctcgccga cttcacggat gccaagtgca tagaggaagc gagcaaaggt
     7141 ggtttctttc gctttttcca gcgcgttaac cacgttttgt gccgattttg gccccatacg
     7201 ctccagtccg gtcagtttgc ctgcggtgag tttgaacaga tccgccggag tgtggacata
     7261 ttctttttca accagctgat cgatgatttt gtcgcccatt ccgtcaacat ccatcgcacg
     7321 gcgggaaaca aagtgtttca gcgactcttt acgctgcgca ccgcaaatca ggccaccggt
     7381 acagcgggca accgcttcac cttccacacg ctcaacgtca gaaccacata ccggacaatg
     7441 cgtcgggaat acaacctcac gggtatcttc cgggcgttca gaaagcacga cgttaaccac
     7501 ctgcgggatc acgtcgccag cgcggcgaat caccacttta tcgccaatgc gtaaaccaag
     7561 acgttcgatt tcatccgcat tgtgtaaggt tgcgttactc accagcacgc cagcaacatg
     7621 gacaggttcc agacgcgcaa caggcgtaat cgcgccagta cgcccaacct gaaactcgac
     7681 gtcacgcaca aaggtcatct gttcctgcgc cggaaattta aacgctaccg cccagcgcgg
     7741 ggcacgcgcg acaaagccaa gctgctcctg ctgtgccagt gagttgactt taatcaccac
     7801 gccgtcgata tcaaagccca gcgtcgggcg gtcttcttcc actttgtgat agaacgccag
     7861 cacttcttcc gccgattcac aaagcgttac ccgatcgctg accggcaacc cccacttttt
     7921 aaattgcagt aagcggccaa gatgagtatc cggcagctcg ccaccttcca gaacaccaac
     7981 gccatagcag aaaaaagtga gcggtcgctt cgctgtaata cgcggatcaa gctgacgcag
     8041 tgaaccagct gccgcattac gtgggttagc aaaaactttc ccgcccgtgc gtcgcgcatc
     8101 ttcgttaatc ttttcgaacc ccgcctgcgg caggaacact tcaccacgca cttccagacg
     8161 cgccgggata ttctctccgt gtagcttcag cggaatggcg cgaatagtac gcacattaga
     8221 cgtgatatct tccccggtgg tgccatcgcc acgcgtcgcg gcactgacta aaacaccatt
     8281 ttcatacaga atactgacgg caagaccatc cagcttcagc tcacagcacc aggtgacttt
     8341 ctcgttgctt ttcagacggt cctgcactcg tttgttgaag gcaagaaagc tttcttcatc
     8401 aaaaacgtta tccagcgaca gcattggcac ttcatggcgg atctggctga aagccgccag
     8461 cggcgcagca cctacacgtt gggtaggcga atcaggcgta atcagttctg gatgtttggt
     8521 ttccagctcg cgcagttcgc gcatcaacct gtcgtattcc gcgtcgggaa tttccggcgc
     8581 atccatcaca tgataaagat attcatgatg gcgaagcgtc gttcgcagtt ctgtcagttg
     8641 ttgttcgatt gattccatat cgcaccatca atgctaaaaa cccccgacaa gcgggggttc
     8701 gaaaaggagt taatttgcct taagtgtatc aggcgttggc gtctttgact tcgcggatga
     8761 tgtcctggta ctcgcgcaat ttctgcggag tcatcatacg gcgctgatcg tcaagcacta
     8821 caccgcccac ttcatcggca atatgctgcg cagattgcag catcagcttg aagttctgca
     8881 gctcgtcacc gtaagacggt acctgcataa agatagtgac acccggagta gtgaaatcct
     8941 tcatttcagg atcaaaggtt cccggtttca ccatattcgc caggctaaat aacgccgggc
     9001 cgctgccatc cgggctaaga tgacgatggt aaatattcat atcgccaaaa atgaagcccg
     9061 cttgttgaat gctgttaaga agcagttcac cgtttaactc gctaccgtga tgcgccgcga
     9121 cgttcatgat aatcaccgct tctttgcgct tcggtttatc cataactgga gccggttccg
     9181 ctacaggctc aggctgtggt gccgctacag gctctgcagg ctggaaagcc tgttgtgccg
     9241 gttgcggtgc tgaatgcact ggctgcggcg ctgatgcgac ctgtggcgaa actggctgct
     9301 gcaacggctg ttcaggctgc ggctgatagg caggctgctg cacgggttgc tgcaccggct
     9361 gcgctggacg cggagcatgt tgcggcggta cctgagcttc aggcggctgc tggaccggtt
     9421 gacgcggctg cgcagacgca taaggcggtt ggtactggtg ttgcggcgac ggacgagcag
     9481 cctcatgctc ctgggcgtta gccggggcat gattcacgcg gtgaactcga acctcaccaa
     9541 cgccctcatc atcttcgaca tcctcgtcat aagaatcgtc gtcacgtttt gacttcattc
     9601 gttttaatgg ccgatcgcgg aacatagaag atcgttcttt acggctggtc cagaaaccat
     9661 gtaccagtaa agcgattatg gcgatcgcgc caacaatgat taatatcaga cgcaaatcct
     9721 gcatcattat attctctgtt gttctaacac cttgccacca cggcaaacat ttactcacta
     9781 agagtatttg ccgattacct caagtgcaag tgcactatta actttcacag cacaaagata
     9841 gatgaaatcg tgctttttgc tgttttttcg aacatatcct aactgtccat tgcgcaatta
     9901 cccggtaaaa tacgcagaat tttcctggga ttggtcaaaa ggagctcatc ctgattatgg
     9961 tttcatcatt cacatctgcc ccacgcagcg gtttttacta ttttgcgcaa ggctggaagc
    10021 tcgtctcgca acctgggatt cggcgtttcg ttattttacc gctgctggtc aatattttgt
    10081 tgatgggggg cgcattctgg tggctattta cgcagctcga tgtctggatc ccgacgctca
    10141 tgagttatgt tccggactgg ttacaatggc tgagctatct gttgtggcct ctggcagtca
    10201 tctccgtgct gttagtgttt ggctatttct tctcaacgat tgctaactgg attgccgctc
    10261 cgttcaacgg tttattggct gaacaactgg aagcaagatt gactggcgct acaccgccag
    10321 ataccgggat tttcggtatc atgaaagatg tgccgcgaat catgaaacgc gaatggcaaa
    10381 aatttgcctg gtatctgccg cgcgcaattg tattgctaat tctttacttc atccctggta
    10441 ttgggcaaac cgtcgcgccg gtactatggt tcctgtttag cgcctggatg ttagccatcc
    10501 agtattgcga ttaccccttc gataaccaca aagtgccgtt taaagagatg cgcaccgccc
    10561 tgcgcacacg caaaatcacc aatatgcagt ttggtgcttt aaccagcctg tttacgatga
    10621 tcccgctgct taatctgttc attatgcccg ttgccgtttg tggcgcgacg gcgatgtggg
    10681 tcgattgcta tcgcgataaa cacgccatgt ggcggtaaca atctaccggt tattttgtaa
    10741 accgtttgta tgaaacaggg gtggcttatg ccgcccctta ttccatcttg catgtcatta
    10801 tttcccttct gcatatagat atgctaaatc cttacttccg catattctct gagcgggtat
    10861 gctacctgtt gtatcccaat ttcatacagt taaggacagg ccatgagtaa gatttttgaa
    10921 gataactcgc tgactatcgg tcacacaccg ctggttcgcc tgaatcgcat cggtaacgga
    10981 cgcattctgg cgaaggtgga atctcgtaac cccagcttca gcgttaagtg ccgtatcggt
    11041 gccaacatga tttgggatgc cgaaaagcgc ggcgtgctga aaccaggcgt tgaactggtt
    11101 gaaccaacca gcggtaatac cgggattgca ctggcctatg tcgctgccgc tcgcggttac
    11161 aaacttaccc tgaccatgcc agaaaccatg agtattgaac gccgcaagct gctgaaagcg
    11221 ttaggtgcaa acctggtgct gaccgaaggt gctaaaggca tgaaaggcgc aatccaaaaa
    11281 gcagaagaaa ttgtcgccag caatccagag aaatacctgc tgctgcaaca attcagcaat
    11341 ccggcaaacc ctgaaattca cgaaaaaacc accggtccgg agatctggga agataccgac
    11401 ggtcaggttg atgtatttat tgctggcgtt gggactggcg gtacgctgac tggcgtcagc
    11461 cgctacatta aaggcaccaa aggcaagacc gatcttatct ctgtcgccgt tgagccaacc
    11521 gattctccag ttatcgccca ggcgctggca ggtgaagaga ttaaacctgg cccgcataaa
    11581 attcagggta ttggcgctgg ttttatcccg gctaacctcg atctcaagct ggtcgacaaa
    11641 gtcattggca tcaccaatga agaagccatt tctaccgcac gtcgtctgat ggaagaagaa
    11701 ggtattcttg ctggtatctc ttctggagca gctgttgccg cggcgttgaa actacaagaa
    11761 gatgaaagct ttaccaacaa gaatattgtg gttattctac catcatcggg tgagcgttat
    11821 ttaagcaccg cattgtttgc cgatctcttc actgagaaag aactgcaaca gtaatgccag
    11881 cttgttaaaa atgcgtaaaa aagcaccttt tcaggtgctt ttttgtggcc tgcttcaaac
    11941 tttcgcccct cctggcattg attcagcctg tcggaactgg tatttaacca gactaattat
    12001 tttgatgcgc gaaattaatc gttacaggaa aagccaaagc tgaatcgatt ttatgatttg
    12061 gttcaattct tcctttagcg gcataatgtt taatgacgta cgaaacgtca gcggtcaaca
    12121 cccgccagca atggactgta ttgcgctctt cgtgcgtcgc gtctgttaaa aactggcgct
    12181 aacaatacag gctaaagtcg aaccgccagg ctagacttta gttccacaac actaaaccta
    12241 taagttgggg aaatacaatg ttccagcaag aagttaccat taccgctccg aacggtctgc
    12301 acacccgccc tgctgcccag tttgtaaaag aagctaaggg cttcacttct gaaattactg
    12361 tgacttccaa cggcaaaagc gccagcgcga aaagcctgtt taaactgcag actctgggcc
    12421 tgactcaagg taccgttgtg actatctccg cagaaggcga agacgagcag aaagcggttg
    12481 aacatctggt taaactgatg gcggaactcg agtaatttcc cgggttcttt taaaaatcag
    12541 tcacaagtaa ggtagggtta tgatttcagg cattttagca tccccgggta tcgctttcgg
    12601 taaagctctg cttctgaaag aagacgaaat tgtcattgac cggaaaaaaa tttctgccga
    12661 ccaggttgat caggaagttg aacgttttct gagcggtcgt gccaaggcat cagcccagct
    12721 ggaaacgatc aaaacgaaag ctggtgaaac gttcggtgaa gaaaaagaag ccatctttga
    12781 agggcatatt atgctgctcg aagatgagga gctggagcag gaaatcatag ccctgattaa
    12841 agataagcac atgacagctg acgcagctgc tcatgaagtt atcgaaggtc aggcttctgc
    12901 cctggaagag ctggatgatg aatacctgaa agaacgtgcg gctgacgtac gtgatatcgg
    12961 taagcgcctg ctgcgcaaca tcctgggcct gaagattatc gacctgagcg ccattcagga
    13021 tgaagtcatt ctggttgccg ctgacctgac gccgtccgaa accgcacagc tgaacctgaa
    13081 gaaggtgctg ggtttcatca ccgacgcggg tggccgtact tcccacacct ctatcatggc
    13141 gcgttctctg gaactgcctg ctatcgtggg taccggtagc gtcacctctc aggtgaaaaa
    13201 tgacgactat ctgattctgg atgccgtaaa taatcaggtt tacgtcaatc caaccaacga
    13261 agttattgat aaaatgcgcg ctgttcagga gcaagtggct tctgaaaaag cagagcttgc
    13321 taaactgaaa gatctgccag ctattacgct ggacggtcac caggtagaag tgtgcgctaa
    13381 cattggtacg gttcgtgacg ttgaaggtgc agagcgtaac ggcgctgaag gcgttggtct
    13441 g
//
LOCUS       DAMZFT010000128        13294 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.128, whole genome shotgun sequence.
ACCESSION   DAMZFT010000128 DAMZFT010000000
VERSION     DAMZFT010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13294)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13294)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13294
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.128"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..173
                     /locus_tag="PN977_003946"
     CDS             <1..173
                     /locus_tag="PN977_003946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HDI6043939.1"
                     /translation="TSMSDIVRDLSRLGWTDERIGTELGMDQDEVLRLKQISGLTELF
                     QEEDFSPAWTVR"
     gene            complement(667..1670)
                     /locus_tag="PN977_003947"
                     /pseudo
     CDS             complement(667..1670)
                     /locus_tag="PN977_003947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
     gene            1763..2784
                     /gene="istA"
                     /locus_tag="PN977_003948"
                     /pseudo
     CDS             1763..2784
                     /gene="istA"
                     /locus_tag="PN977_003948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627495.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
     gene            2781..3562
                     /gene="istB"
                     /locus_tag="PN977_003949"
                     /pseudo
     CDS             2781..3562
                     /gene="istB"
                     /locus_tag="PN977_003949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858162.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element IS100kyp family helper ATPase
                     IstB"
     gene            complement(3634..4169)
                     /locus_tag="PN977_003950"
                     /pseudo
     CDS             complement(3634..4169)
                     /locus_tag="PN977_003950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(4189..4509)
                     /gene="tnpB"
                     /locus_tag="PN977_003951"
     CDS             complement(4189..4509)
                     /gene="tnpB"
                     /locus_tag="PN977_003951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HDI6043940.1"
                     /translation="MVNYRLISLALTGFNGLSSKVQNVLKDDPFSGHIFVFRGRSGKM
                     VKILWADRDGLCLFAKRLERGRFIWPVTREGKVHLTPAQLSMLLEGIAWQHPKRTERP
                     GIRI"
     gene            4575..5743
                     /gene="istA"
                     /locus_tag="PN977_003952"
                     /pseudo
     CDS             4575..5743
                     /gene="istA"
                     /locus_tag="PN977_003952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000952428.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
     gene            5743..6270
                     /locus_tag="PN977_003953"
                     /pseudo
     CDS             5743..6270
                     /locus_tag="PN977_003953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000544830.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            6408..7484
                     /locus_tag="PN977_003954"
                     /pseudo
     CDS             6408..7484
                     /locus_tag="PN977_003954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907654.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            7501..7644
                     /locus_tag="PN977_003955"
                     /pseudo
     CDS             7501..7644
                     /locus_tag="PN977_003955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611483.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
     gene            8348..9607
                     /gene="amiC"
                     /locus_tag="PN977_003956"
     CDS             8348..9607
                     /gene="amiC"
                     /locus_tag="PN977_003956"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000961390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiC"
                     /protein_id="HDI6043941.1"
                     /translation="MLVSNYNYVISRRRLLQGAGAMWLLSVSQVSLAAVSQVVAVRVW
                     PASSYTRVTVESNRQLQYKQFALSNPERVVVDIEDVNLNSVLKGMAAQIRADDPFIKS
                     ARVGQFDPQTVRMVFELKQNVKPQLFALAPVAGFKERLVMDLYPANAQDMQDPLLALL
                     EDYNKGDLEKQVPPAQSGPQPGKAGRDRPIVIMLDPGHGGEDPGAVGKYKTREKDVVL
                     QIARRLRSLIEKEGNMKVYMTRNEDIFIPLQVRVAKAQKQRADLFVSIHADAFTSRQP
                     SGSSVFALSTKGATSTAAKYLAQTQNASDLIGGVSKSGDRYVDHTMFDMVQSLTIADS
                     LKLGKAVLNKLGKINKLHKNQVEQAGFAVLKAPDIPSILVETAFISNVEEERKLKTAR
                     FQQQVAESILEGIKEYFSDIDALARKA"
     gene            complement(9767..10454)
                     /locus_tag="PN977_003957"
                     /pseudo
     CDS             complement(9767..10454)
                     /locus_tag="PN977_003957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012539895.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            10530..11001
                     /locus_tag="PN977_003958"
                     /pseudo
     CDS             10530..11001
                     /locus_tag="PN977_003958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001028172.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(11059..11942)
                     /locus_tag="PN977_003959"
                     /pseudo
     CDS             complement(11059..11942)
                     /locus_tag="PN977_003959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001608690.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            12012..13000
                     /locus_tag="PN977_003960"
                     /pseudo
     CDS             12012..13000
                     /locus_tag="PN977_003960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001179707.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
BASE COUNT         3450 a         3179 c         3281 g         3384 t
ORIGIN      
        1 taacgtcaat gtctgacatt gtcagggacc tctcacgtct gggctggaca gatgagcgta
       61 ttggcacaga gttgggcatg gaccaggatg aagtgctgag gctgaaacag attagcgggc
      121 tgacagagct gtttcaggaa gaggatttca gccctgcatg gacggttcgt taagggctat
      181 gttctgacga gagtgtaatt taaattttgt aattgcctgt ttttgatatg ttccctacac
      241 cgtggagaca ggcaattgat aaacaagaat ccaatgctat tgctgccgaa ctggtaaatt
      301 gccttaaaac cgaatctgat cttaacctgt ttttatacat gcttacgaag tgtactgtta
      361 aagatgagct tagcgctgaa ctcacagagc atcttggaca tgagaacacc tgagaataaa
      421 ggcagcaatg caagtaaaga ctactcgatt aaaacgctgc tcataaatga tgccctaaca
      481 atttgtgaaa tagtatttcg tccgggtgtt cgctttgttt atgtcgttac ggggaagtca
      541 tcttcacagg gatttttagt cagttagcat taccgatatc cattcactaa cttaccggag
      601 ataattgatg tcaaaacccc aatggaccat tgaacagtaa gcggttcgcc agaaccgtat
      661 tgatatttac tgagagctca gatcaacttt ccagggcaac agatcgcgta cccggtttgt
      721 cggccagtcc tggatatgct caatgacgta acgcagccat ttttctggtt caacattgtt
      781 cagacggcat gtgccgatca gcgagtacaa caccgccgca tgttcgccac cgctgtcgga
      841 acccgcgaac aaccagtttt tccggcctac gtccactccc cgtaaggcgt tctctgcgat
      901 gttgttgtcg atttccaccc agccattact gcagtacacg ttcaggctat ctcactgttt
      961 cagcaggtag gcgaacgctt ttaccgtatc cgagtgacgc gacagtgttt tcatctgggt
     1021 ctgtatccag tcatacagtg actgcatcag tggcgcagct ctggcttttc ttgccgccag
     1081 acgctgttct gctgtacatc cccggacttc tgcctcgatg gcatacagtt caccgatacg
     1141 ctgcagggct tccgtggtga tgtcagttgg cgctcttgca tgcaaatcgt ggattttcct
     1201 ccgggcatgg gccatacacg cggcttccgt tattctgccg gattcgtata acgcccggta
     1261 accaccgtaa gtatcggcct gaagcacacc gctgtaaccg gccagatgat tctgtggatg
     1321 gatacctttc cggtcctgac tgtacgcgaa ccagaccgcc gggggcatct ccgacccggc
     1381 attacggtca tcacggacgt agacccacag cctgtccgtt cggtttttac cactgcctgg
     1441 ctcctggacc ggaacgggga tatcatcagc atggacttta ccgggcatca gcacatactg
     1501 gcgcaggacg tcatacagcg gctccagcag ttcagcaacg gtgtcagctc cacgtcctga
     1561 ctgcggtata tttctgactg acggtataac ggcagatggt ctgcatactt cccggtgaca
     1621 acatgggcca gaagccccgc tccggcataa ctgtgtgcaa tgggttttga atgagctgtc
     1681 acgaacggtg caatagtgat ccacacccaa cgcctgaaat cagatccagg gggtaatctg
     1741 ctctcctgat tcaggagagc ttatggtcac ttttgagaca gttatggaaa ttaaaatcct
     1801 gcacaagcag ggaatgagta gccgggcgat tgccagagaa ctggggatct cccgcaatac
     1861 ggttaaacgt tatttgcagg caaaatctga gccgccaaaa tatacgccgc gacctgctgt
     1921 tgcttcactc ctggatgaat accgggatta tattcgtcaa cgcatcgccg atgctcatcc
     1981 ttacaaaatc ccggcaacgg taatcgctcg cgagatcaga gaccagggat atcgtggcgg
     2041 aatgaccatt ctcaggatat tcattcgttc tctctcggtt cctcaggagc aggagcctgc
     2101 cgttcggttc gaaactgaac ccggacgaca gatgcaggtt gactggggca ctctgcgtaa
     2161 tggccgctca ccgcttcacg tgttcgttgc tgttctcgga tacagccgaa tgttgtacat
     2221 cgaattcact gacaatatgc gttatgacac gctggaaatc tgccatcgta atgcgttccg
     2281 cttctttggt ggtgtgccgc gcgaagtgtt gtatgacaat atgaaaactg tggttctgca
     2341 acgtgacgca tatcagaccg gtcagcaccg gttccatcct tcgctgtggc agttcggcaa
     2401 ggagatgggc ttctctcccc gactgtgtcg ccccttcagg gcacagacta aaggtaaggt
     2461 ggaacggatg gtgcagtaca cccgtaacag tttttacatc ccactaatga ctcgcctgcg
     2521 cccgatgggg atcactgtcg atgttgaaac agccaaccgc cacggtctgc gctggctgca
     2581 cgatgtcgct aaccaacgaa agcatgaaac aatccaggcc cgtccctgcg atcgctggct
     2641 cgaagagcag cagtccatgc tggcactgcc tccggagaaa aaagagtatg acgtgcatcc
     2701 tggtgaaaat ctggtgaact tcgacaaaca cccctgcatc atccactctc catctacgac
     2761 tcattctgca gaggagtggc gtgatgatgg aactgcaaca tcaacgactg atggcgctcg
     2821 ccgggcagtt gcaactggaa agccttataa gcgcagcgcc tgcgctgtca caacaggcag
     2881 tagaccagga atggagttat atggacttcc tggagcatct gcttcatgaa gaaaaactgg
     2941 cacgtcatca acgtaaacag gcgatgtata cccgaatggc agccttcccg gcggtgaaaa
     3001 cgttcgaaga gtatgacttc acattcgcca ccggagcacc gcagaagcaa ctccagtcgt
     3061 tacgctcact cagcttcata gaacgtaatg aaaatatcgt attactgggg ccatcaggtg
     3121 tggggaaaac ccatctggca atagcgatgg gctatgaagc cgtccgtgca ggtatcaaag
     3181 ttcgcttcac aacagcagca gatctgttac ttcagttatc tacggcacaa cgtcagggcc
     3241 gttataaaac gacgcttcag cgtggagtaa tggccccccg cctgctcatc attgatgaaa
     3301 caggctatct gccgttcaat caggaagaag caaaactgtt cttccaggtc attgctaaac
     3361 gttacgaaaa gagccaatga tcctgacatc caatctgccg ttcgggcagt gggatcaaac
     3421 gttcgccggt gatgcagcac tgacctcagc gatgctggac cgtatcttac accactcaca
     3481 tgtcgttaaa atcaaaggag aaagctatcg actcagacag aaacgaaaag ccggggttat
     3541 agcagaagct aatcctgagt aaaacggtgg atcaatattg ggccgttggt ggagatataa
     3601 gtggatcact tttcatccgt cgttgacatg agccggcgcc tgcacgatat ggtcacaccg
     3661 acaacaggcc agtttcggac ttgtgtttcg ataaccttaa aggcgcagct gataagctcc
     3721 agctgctctg acacatcaca tcccagagaa ctgagttcac caccacaggc aggacagcat
     3781 tcctcttctg gccggataac ccgggtttca cggggaagtg aggccggtaa ctgtttacag
     3841 gctgaagact gacgcagggc ggcggatggc agtaccgggt catattgctc acccagcgtt
     3901 tccgccattt cttcctgaag tgtgctgatt ctctcctgtg cttcctgtat ctgtcgttcg
     3961 gtttttgcgc gaagtttttc tgagctttta ccgaaatgca tacgttgcag tttcgcaacc
     4021 agcgccttca gccggttgat ttcagaagca taagccgcca ccctctgtga gagcaggcgg
     4081 ttgtattcag ccatctggcg gatggtgtcc tgttgcgtct gcaacagtgc ccgcaggcgg
     4141 ggattctcat gagcaagtga ggtgtccata tactcgcttt gcaacgggtt atatccggat
     4201 gccaggccgt tctgtccgtt tgggatgttg ccacgcgatc ccctccagta gcatggataa
     4261 ctgagctggc gtcaggtgca ctttcccttc ccgggtcacc ggccagatga agcggccccg
     4321 ttccaggcgt ttggcgaaca ggcataaccc atcacgatcg gcccacagta ttttcaccat
     4381 tttgccactg cggccccgga agacgaagat atgcccggag aacgggtcat ccttcaggac
     4441 gttctgaact tttgaggaca ggccgttaaa gcctgtcagc gccagtgata taagacggta
     4501 attcaccatt tggattgtcc gctccaccca acatgttgtt tccttaaggt tctcacacca
     4561 gaaaggacat caacatgctg agcagagagg acttttacat gataaagcaa atgcgccagc
     4621 agggcgcgtt gtcgatattg tgactcagat tggttgctct gaacgaacgg tcagacgcta
     4681 cctcaaatac cctgaaccgc cagccagaag gacccgccac aaaatggtta agctgaaacc
     4741 gtttatggat tacatcgaca tgcgcctggc agagaatgtc tggaatagcg cggttatctt
     4801 cgcggagatt aaggcaatgg gctatacggg cggacgttcc atgctgcgtt actacatcca
     4861 gcccaaacgt aaaatgcgtc cgtcaaaaag aacagttcgc ttcgaaactc agcctggata
     4921 ccagctccag cacgactggg gcgaagttga ggtggaagtt gccgggcaac ggtgcaaagt
     4981 taactttgcg gttaatgcgc tggggttctc ccgccgcttt catgtcttcg ctgccccaaa
     5041 acaggatgct gagaatacct acgaatcact ggttcgcgcc ttccgctact tcggtggttg
     5101 tgtgaaaacg gtgctggttg ataaccagaa ggctgcggtg ctgaagaata acaacgggaa
     5161 agtcgtgttc aactccggat ttctgttgct ggctgaccac tataacttcc tgccacgggc
     5221 atgccgtcca cgcagggcca gaacaaaagg taaggttgag cggatggtga aatacctcaa
     5281 ggagaacttc ttcgtccggt accgcaggtt cgacagcttc actcatgtca atcaacaact
     5341 ggagcaatgg atagccgatg tggctgacaa acgggagctt cgtcagttca aagaaacgcc
     5401 ggaacagcgc ttcgcgctgg agcaggaata tctgcagccg ttaccggata cggacttcga
     5461 taccagttac tttgacatcc gccatgtgtc ctgggacagc tatatcgagg ttggtggtaa
     5521 tcgttacagc gttcccgaag cgctgtgtgg tcagccggta tcgatacgaa tatcgctgga
     5581 tgacgagttg cggatctaca gtaatgagaa actggtggcc tcacatcgcc tctgttcggc
     5641 atcgtctggc tggcagacag tgccggagca tcacaccccg ctctggcagc aggtcagtca
     5701 ggtggaacat cgactactga gtgcctatga ggagctgttg tgatgcatga gctggaagtc
     5761 ctgctgagtc gcctgaaaat ggagcatctg agttatcacg ttgaaagcct gctggaacag
     5821 gcagctaaaa aagagctgaa ctaccgggag ttcctgtgca tggcgctaca gcaggaatgg
     5881 aacggcaggc atcagcgcgg tatggagtcc aggctgaagc aggctcgtct gccgtgggtc
     5941 aaaacgctgg agcagttcga ctttaccttc cagccgggca tctaccgtaa ggttgtccgg
     6001 gaactggctg gtctggcgtt cgtggagcgc agcgaaaacg tgatcctgct gggacctcct
     6061 ggtgtcggaa aaactcatct ggccatagct cttggcgtga aagcggtgga tgcgggacat
     6121 cgggtactgt ttatgccact ggacagactg atcgcgacac tgatgaaagc gaaacaggaa
     6181 aaccggctgg agcgtcagct acagcaactg agttatgccc gggtgttgat cctggatgaa
     6241 ataggctatc tgccgatgaa cagagaggat gtcggaaacg ccttcatgcg aggcgtctcc
     6301 tcaccgatgc gcgtgactca agaagggcct gacgtcttgt ctcgttactg tcctgtccgg
     6361 gttatctgtc tggagattca actctgtttc ctcacaggag ctctgttatg gcaggtaaag
     6421 ttacggaaac cgctgttgtg ggtggcgtgg atatacataa agatctgcac gttgccgctg
     6481 tcgtagatca gaacaataaa gttctggggc ccccgttttt ctccacaaca cggcaaggtt
     6541 accggcagat gctggcatgg atggtttcgt ttggggcatt aaagcgaatt ggtgttgagt
     6601 gtacaggcac ctatggatca ggtctgcttc gctatttaca gaatgccggg ttagaagttc
     6661 ttgaggtggc tgtgccagat cggatggagc gacgcaaacg gggtaaaagt gacacgattg
     6721 atgctgaatg tgccgctcac gccgcatgct ccggaataag aactgtcaca cccaaaacgc
     6781 gcaatggtat gattgagtct ctgcgggtat taaaaacttg ccgaaaaaca gcaatatcag
     6841 cccgcagagt cgctctccag attaaccatt ccaatattat ctctgccccg gatgaattac
     6901 gtgaacagct cagaaatatg acgcgcatgc agctcatcag gactctgaga tcctagcggc
     6961 ctgatgccag tgaatagctc aatgttacca acgtttatcg tatttcatta aagtcccttg
     7021 cccgacgcta tctcgagtta catgacgaaa tcgctgattt ggatgttatg attgcggcaa
     7081 ttgtggatga gctggcacct gcactgatta aacgtaatgc tattggatac gaaagcgctt
     7141 cacagttgct gatcactgcc ggagacaatc cccaacgatt aagatcagaa tcaggttttg
     7201 cggcactgtg tggtgtcagc cctgttcccg tatcttcagg aaaaacgaat cgttatcgac
     7261 ttaaccgggg tggagatcgt gctgcaaata gtgcacttca catcatttcc atcggacgtt
     7321 tgcgaactga cgataaaacg aaggaatatg tcgccagacg agtagcggaa gggcatacaa
     7381 aaatggaaac aatacgcagc ctgaagcgct atatctcaca cgaagtttat acattactgc
     7441 gtaatcaaaa caggcagatc aacagcaccc cgataactgc ttgactctta gaagggcgtc
     7501 cagggcagtc attatacgcg taggcagttc cggcagttat tgtggcgata ccagatcagg
     7561 cagagtatga gccggcgcgg aaactgctgg gataacagcc caatggaacg cttcttcagg
     7621 agtctgaaga acgaatggac gccccgctct tcaactttgg taataccaaa atcagacgac
     7681 ggtgttatag gtgcttgaac tgaatttact ttgaaaaaat gtaaaaacat ttaaatcata
     7741 gccagaagcc gcgattattc agtgcttctc attttatatt ttctgcaaat ttatatgtgg
     7801 tataaaataa cacccttgtc attcaggtat aaaatatcat catcgtacag taaaggatat
     7861 ataaatataa aattatacta ttcaaaatat ggtgcattct tattcatgat aggaatactt
     7921 agaacatcat tttttcattg tagaccatga tttcggattt gaaaagttcc ttccccagta
     7981 atctctagtt tttattctca attcaaccca actcttccct atacaaacta aaaaacattg
     8041 ttctattgat agatacttct cagtaatcag ataaaaacta taatattgct gtgcatgatg
     8101 tacatattta aaatgtcatg agtacatccc ttttatttga aaaacaaact aaaagaaatc
     8161 acttaaataa aatgttttta tatcgtggta tttaagaaaa cttatatcaa aaaataagat
     8221 attgaccttt atttaaaatt agattagatt gcaaagataa gaatacgtgt gattatttct
     8281 ttaaacacct gtaaaaattt attagagcat agttatatta ttgaacattg tattgcttag
     8341 gtaaggtatg ctggtatcta actataatta tgtaatcagc cgtcgtcgtt tactgcaagg
     8401 tgcgggcgcc atgtggctat tgagcgtaag tcaggtcagc ctggctgcgg tcagccaggt
     8461 cgtggcggta cgcgtctggc ctgcgtccag ctacacccgc gtgacggtag aatcaaatcg
     8521 tcagctgcaa tataagcagt tcgcgttaag taaccctgaa cgtgtggtgg tggatatcga
     8581 agatgtaaac ctgaactcgg tactcaaggg gatggcggca caaattcgtg cagacgaccc
     8641 gttcatcaag tcggcgcgcg tcgggcaatt tgacccgcaa accgtacgca tggtttttga
     8701 attaaagcaa aacgtaaaac cgcagctgtt tgcccttgcg ccggtcgccg ggtttaaaga
     8761 gcgtctggtg atggacctct atccggccaa tgcacaggat atgcaggacc cgctgctggc
     8821 gctgctggag gattacaaca aaggcgacct cgaaaagcag gtgccgccag cacaaagtgg
     8881 tccacaaccg ggtaaagcag ggcgtgatcg tccgattgtc attatgcttg accccggcca
     8941 cggtggcgaa gaccccggtg cggtggggaa atacaaaaca cgcgaaaaag acgtggtatt
     9001 gcaaatagct cgccgtctgc gctctctgat cgaaaaagag ggtaatatga aggtgtacat
     9061 gacgcgcaat gaagacatct tcattccgtt gcaagtgcgt gtagcaaaag cacagaaaca
     9121 acgtgctgac ctttttgttt ctatccatgc tgatgccttt accagtcgcc agccgagcgg
     9181 ttcttccgtg tttgcgcttt cgaccaaagg cgcgacaagt actgcggcaa aatatctggc
     9241 acaaacccag aacgcctcgg acttgattgg cggcgtgagc aaaagcggtg accgctatgt
     9301 cgaccacacc atgttcgata tggtgcagtc tctgaccatt gccgacagcc tgaagttggg
     9361 taaagcggtg ctgaataagc tcggtaaaat caacaagctg cataaaaatc aggttgaaca
     9421 ggccgggttt gccgtgctga aagcacctga tattccctcc attctggtcg aaacggcgtt
     9481 tatcagtaac gttgaggaag agcgcaaact gaaaacggca agattccaac aacaagtggc
     9541 agaatctatc ctagagggaa ttaaagagta tttttcggat atagacgcgt tagcgagaaa
     9601 agcataattt aaaaatgttt aatattctat gttctcggtc atattttatt aatttggatg
     9661 aatatatgct gttagtgctg tattctccgt ttggatacac ctttacctta gcgatatagt
     9721 tgtgttattc atatataaat atgccccgtt ttaattccat gcatttttat atcttggtca
     9781 aataatggtg tcgtcgatat tcttccagcg tcttattgtt cagtgattca tgtgtgcgtt
     9841 catagctata ttctgataac catttctccg taatttctag aacttcattc agcgttctga
     9901 acagataaaa atcgagtatt tctgtacggt gtgtcctgtt aaaacgccca ataaaagcgt
     9961 tctacgtcag cttacccggc tggataaact cccgttttac tgcgtgtata tcagcccatt
    10021 cagccagcgt tagagagata aattccgaac cattatctat gcgcagcatg accggataac
    10081 tgcgatttgc cgcgaacctg ctgcctcaga acctgaaaaa gttttgaaaa accgtatcga
    10141 ggatatcgct cgtccactgc ctgcaccacc acaataacgg ggtcgtacca cgtggtatcc
    10201 ggacggtaat gggaaaccgt tctgctctgg ttcagactcc ggcaggcctg atggacactg
    10261 agtccaaatg tcgttatctg atgagtaaca agctcacgct tacaggctgg ttttaaagct
    10321 tttttcgata atgtctttca atgccggttc tctaggctaa ggtcggcaag catctgtttt
    10381 ggacgtcggt tctcgtcctc aagatcctcg ttctttttaa tatcaggtga ctctatgcat
    10441 cgtatctggg cttccagttc atggagtgga gtaccccctc cttttgcggt taactgtacg
    10501 aaatgcactc actcagcagg ggaatcacaa tgaatatcgt attcctaggt attgatctgg
    10561 ataaaaatat ctttcagctc tgcgggttaa attaggtcgg taatccggtt tattcaaaac
    10621 gtattggccg aaaagaatta ctccaaatag tggcgaatat tccggccttc ctaattgatg
    10681 tagggcatcc acatgggaat tttactggca gagtgaattt aaaaaactgg ggcataacgt
    10741 aaaggtcctc aacccacagt atgtaactcc ctttgtctgt gggcaaaaaa tgacggtaat
    10801 gatgcacagg ccatcgcagt gcttctgatg taaccgacaa tgctgttcat gcctccaaaa
    10861 agctcggaac aacaggatat ctacgctttg caccgggcaa ggcagcatat tgttaatgtg
    10921 accgtttgtc aaatcagggg actgttgctt gattggggaa taactatcgg cgcggtagtt
    10981 tcaagagatc gtcgtgctat gtcaacgcca agattattgg ccgttttggg gatgcggacg
    11041 atttcctgga cttctttatt attccagcct acattgttgg cggtacattt cagggctgtc
    11101 ttcacccagc gataatttta tacgccgctc gttataccct ctgatgtaag catccacatg
    11161 ctgcatgaac ttttccggca tgatgcccga ccatttacgc ccataataca tttccgtttt
    11221 cagtctgccg aaaaagcctt cgcatgcggc atcatcctta cgggacattg agtgaataag
    11281 acctgcggca ttaactcttt ccagccagcc tggccagcga taatgcccac ctctgccact
    11341 gtgtatcatc ggtcgttcgc cagcatttaa cgttttgaca gcgttatcca gcatagcgtt
    11401 aaccagttct gcgtcggggc gtgtactgag agaccagctc acaacttttc catcgaagca
    11461 gtccaccacc tgtgacagcc agacttttcc tgcaggaagc tggaactccg ttatatctgt
    11521 cagccatttc tgattaggtt ggtcctcctt aaaatctctg gcgataaggt tatccggatc
    11581 cggtccgatt tctccgcaat atgagctgta gcggcgacga cggttacggc tgacgacaag
    11641 ctgttccttc accataagtc tgcttacaac cttttctgat agctgacccc ctcatgcctg
    11701 agcatcgcat gcaggcgacg atagccataa cactggtaat tactgttaac tatatctgtc
    11761 agcatcgtac gtatcgtggc gtatttatca ccggctttca gtgcagcccg gtgataaaaa
    11821 taactgctac gggcaagccc cagaacatgc agtaattctg tcaggggata tgtttgtctc
    11881 agggcatcag cgatatttgt tttttctctg ttgtttaggt gactgatact gatgcccggg
    11941 tctaatgaga tggtcacccc caccctgtga gcggtacgct gacagggtgg gttattttag
    12001 agggcaccat catgtaaaac gttgcgctta ttggtatcga tctcggtaaa cattctttcc
    12061 atattcattg tcaagataaa tctggcaatg cgcttttgcg taaaaagttt acgcgtacga
    12121 agctgatgca gtttctggca acatgtccat cctcagtagt tgttatggaa gcctgcgctg
    12181 gtacgcattt tatggcccgg cgtatcagcg acatcggtca tgaagaaaaa cttatctctc
    12241 cgtaattcgt gcgtccgttt gtcaaaggta ataagaatga ttttattgat gcagaagcca
    12301 tatgcaaagc ggcttcacga ccatcaatgc gttttgtcca gcccagaaca gaaacccagc
    12361 aggcaatgag ggcattacat cgggtcagag aatcgctcat cagagataag gtgaaaaaaa
    12421 acaaccagat ccacggtttc ttgctggaat ttggaatcag actgccgaca ggggatgccg
    12481 caataaaatg gttatatctg gcgttagctg aacacgaaat acctgaatat ctgagtcgct
    12541 tgctgatgaa attacatgct cactatcttt atcgtattga gcaaataaca gaactggaat
    12601 cagagttaaa ccagtcaatc aaagctgacg ctactgtgca acgaatcatg acaatccccg
    12661 gtgttgggcc gatgacggcc agcctgctat catcccagct tggtgatggt aaacaatttt
    12721 cgtgcagtag ggattttgca gcatccacag gacttgttcc ccgacaatac agtaccggtg
    12781 gtaaatcaat gctgctgggg atcagcaaac gagtcggtta cttgttctgt acgcccgctc
    12841 gttcatgatg cggctggagc atcaataggg cagagttgct gaatgggttc aaaagcaact
    12901 cgacaataag cattcaaata ttgtcgcctg cgctctggca aataaactgg cccgaatagc
    12961 ctgggcaatc actgctctgc acaaagaata tcaggcctga tatagatact gacaaaactc
    13021 aattagctta ttttaaaaaa taagatacaa gttacctgtc tggttttgcg aagactgatt
    13081 attgatgaca tgaacggcaa atagacctga ttaaaaacct gacagaaaaa atggctcact
    13141 gaagccgagg gtctttttag gattatcagg tgcggaactc atcgaggcgc gggtctcctt
    13201 gtccacagag acgccggata gatttaagca agcccaccat taatcaaaaa tcagtgtagc
    13261 aaaaacgggg gtgaccatag attttttatg attt
//
LOCUS       DAMZFT010000129        12997 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.129, whole genome shotgun sequence.
ACCESSION   DAMZFT010000129 DAMZFT010000000
VERSION     DAMZFT010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12997)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12997)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12997
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.129"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            95..1342
                     /gene="mdtA"
                     /locus_tag="PN977_003961"
     CDS             95..1342
                     /gene="mdtA"
                     /locus_tag="PN977_003961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter subunit MdtA"
                     /protein_id="HDI6043942.1"
                     /translation="MKGSYKSRWVIVIVVVIAAIAAFWFWQGRNDSRSAAPGATKQAQ
                     QSPAGGRRGMRSGPLAPVQAATAVEQAVPRYLTGLGTITAANTVTVRSRVDGQLMALH
                     FQEGQQVKAGDLLAEIDPSQFKVALAQALGQLAKDKATLANARRDLARYQQLVKTNLV
                     SRQELDAQQALVSETEGTIKADEASVASAQLQLDWSRITAPVDGRVGLKQVDVGNQIS
                     SGDTTGIVVITQTHPIDLVFTLPESDIATVVQAQKAGKPLVVEAWDRTNSKKLSEGTL
                     LSLDNQIDATTGTIKVKARFNNQDDALFPNQFVNARMLVDTEQNAVVIPTAALQMGNE
                     GHFVWVLNSENKVSKHLVTPGIQDSQRVVIRAGISAGDRVVTDGIDRLTEGAKVEVVE
                     AQSATTSEEKATSREYAKKGARS"
     gene            1342..4464
                     /gene="mdtB"
                     /locus_tag="PN977_003962"
     CDS             1342..4464
                     /gene="mdtB"
                     /locus_tag="PN977_003962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtB"
                     /protein_id="HDI6043943.1"
                     /translation="MQVLPPSSTGGPSRLFIMRPVATTLLMVAILLAGIIGYRALPVS
                     ALPEVDYPTIQVVTLYPGASPDVMTSAVTAPLERQFGQMSGLKQMSSQSSGGASVITL
                     QFQLTLPLDVAEQEVQAAINAATNLLPSDLPNPPVYSKVNPADPPIMTLAVTSTAMPM
                     TQVEDMVETRVAQKISQISGVGLVTLSGGQRPAVRVKLNAQAIAALGLTSETVRTAIT
                     GANVNSAKGSLDGPSRAVTLSANDQMQSAEEYRQLIIAYQNGAPIRLGDVATVEQGAE
                     NSWLGAWANKEQAIVMNVQRQPGANIISTADSIRQMLPQLTESLPKSVKVTVLSDRTT
                     NIRASVDDTQFELMMAIALVVMIIYLFLRNIPATIIPGVAVPLSLIGTFAVMVFLDFS
                     INNLTLMALTIATGFVVDDAIVVIENISRYIEKGEKPLAAALKGAGEIGFTIISLTFS
                     LIAVLIPLLFMGDIVGRLFREFAITLAVAILISAVVSLTLTPMMCARMLSQESLRKQN
                     RFSRASEKMFDRIIAAYGRGLAKVLNHPWLTLSVALSTLLLSVLLWVFIPKGFFPVQD
                     NGIIQGTLQAPQSSSFANMAQRQRQVADVILQDPAVQSLTSFVGVDGTNPSLNSARLQ
                     INLKPLDERDDRVQKVIARLQTAVDKVPGVDLFLQPTQDLTIDTQVSRTQYQFTLQAT
                     SLDALSTWVPQLMEKLQQLPQLSDVSSDWQDKGLVAYVNVDRDSASRLGISMADVDNA
                     LYNAFGQRLISTIYTQANQYRVVLEHNTENTPGLAALDTIRLTSSDGGVVPLSSIAKI
                     EQRFAPLSINHLDQFPVTTISFNVPDNYSLGDAVQAIMDTEKTLNLPVDITTQFQGST
                     LAFQSALGSTVWLIVAAVVAMYIVLGILYESFIHPITILSTLPTAGVGALLALMIAGS
                     ELDVIAIIGIILLIGIVKKNAIMMIDFALAAEREQGMSPREAIYQACLLRFRPILMTT
                     LAALLGALPLMLSTGVGAELRRPLGIGMVGGLIVSQVLTLFTTPVIYLLFDRLALWTK
                     SRFARHEEEA"
     gene            4465..7542
                     /gene="mdtC"
                     /locus_tag="PN977_003963"
     CDS             4465..7542
                     /gene="mdtC"
                     /locus_tag="PN977_003963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtC"
                     /protein_id="HDI6043944.1"
                     /translation="MKFFALFIYRPVATILLSVAITLCGILGFRMLPVAPLPQVDFPV
                     IMVSASLPGASPETMASSVATPLERSLGRIAGVSEMTSSSSLGSTRIILQFDFDRDIN
                     GAARDVQAAINAAQSLLPSGMPSRPTYRKANPSDAPIMILTLTSDTYSQGELYDFAST
                     QLAPTISQIDGVGDVDVGGSSLPAVRVGLNPQALFNQGVSLDDVRTAISNANVRKPQG
                     VLEDGTHRWQIQTNDELKTAAEYQPLIIHYNNGGAVRLGDVATVTDSVQDVRNAGMTN
                     AKPAILLMIRKLPEANIIQTVDSIRARLPELQSTIPAAIDLQIAQDRSPTIRASLEEV
                     EQTLIISVALVILVVFLFLRSGRATIIPAVAVPVSLIGTFAAMYLCGFSLNNLSLMAL
                     TIATGFVVDDAIVVLENIARHLEAGMKPLQAALQGTREVGFTVLSMSLSLVAVFLPLL
                     LMGGLPGRLLREFAVTLSVAIGISLLVSLTLTPMMCGWMLKASKPREQKRLRGFGRML
                     VALQQGYGKSLKWVLNHTRLVGVVLLGTIALNIWLYISIPKTFFPEQDTGVLMGGIQA
                     DQSISFQAMRGKLQDFMKIIRDDPAVDNVTGFTGGSRVNSGMMFITLKPRGERSETAQ
                     QIIDRLRVKLAKEPGANLFLMAVQDIRVGGRQSNASYQYTLLSDDLAALREWEPKIRK
                     KLATLPELADVNSDQQDNGAEMNLVYDRDTMARLGIDVQAANSLLNNAFGQRQISTIY
                     QPMNQYKVVMEVDPRYTQDISALEKMFVINNEGKAIPLSYFAKWQPANAPLSVNHQGL
                     SAASTISFNLPTGKSLSDASAAIDRAMTQLGVPSTVRGSFAGTAQVFQETMNSQVILI
                     IAAIATVYIVLGILYESYVHPLTILSTLPSAGVGALLALELFNAPFSLIALIGIMLLI
                     GIVKKNAIMMVDFALEAQRHGNLTPQEAIFQACLLRFRPIMMTTLAALFGALPLVLSG
                     GDGSELRQPLGITIVGGLVMSQLLTLYTTPVVYLFFDRLRLRFSRKPKQTVTE"
     gene            7543..8958
                     /locus_tag="PN977_003964"
     CDS             7543..8958
                     /locus_tag="PN977_003964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDI6043945.1"
                     /translation="MTDLPDSTRWQLWIVAFGFFMQSLDTTIVNTALPSMAQSLGESP
                     LHMHMVIVSYVLTVAVMLPASGWLADKVGVRNIFFTAIVLFTLGSLFCALSGTLNELL
                     LARALQGVGGAMMVPVGRLTVMKIVPREQYMAAMTFVTLPGQVGPLLGPALGGLLVEY
                     ASWHWIFLINIPVGIIGAIATLMLMPNYTMQTRRFDLSGFLLLAVGMAVLTLALDGSK
                     GTGLSPLAIAGLVAVGVVALVLYLLHARNNNRALFSLKLFRTRTFSLGLAGSFAGRIG
                     SGMLPFMTPVFLQIGLGFSPFHAGLMMIPMVLGSMGMKRIVVQVVNRFGYRRVLVATT
                     LGLSLVTLLFMTTALLGWYYVLPFVLFLQGMVNSTRFSSMNTLTLKDLPDNLASSGNS
                     LLSMIMQLSMSIGVTIAGLLLGLFGSQHVSVDSGTTQTVFMYTWLSMAFIIALPAFIF
                     ARVPNDTHQNVAISRRKRSAQ"
     gene            8955..10358
                     /gene="baeS"
                     /locus_tag="PN977_003965"
     CDS             8955..10358
                     /gene="baeS"
                     /locus_tag="PN977_003965"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     BaeS"
                     /protein_id="HDI6043946.1"
                     /translation="MKFWRPGITGKLFLAIFATCIVLLITMHWAVRISFERGFIDYIK
                     HGNEQRLQMLGDALGEQYAQHGNWRFLRNNDRFVFQILRSLEHDNNEDKPGPGMPPHG
                     WRTQFWVVDQNNKVLVGPRAPIPPDGTRRPIMVNGAEVGAVIASPVERLTRNTDINFD
                     RQQRQTSWLIVALSTLLAALATFPLARGLLAPVKRLVDGTHKLAAGDFTTRVTPTSED
                     ELGKLAQDFNQLASTLEKNQQMRRDFMADISHELRTPLAVLRGELEAIQDGVRKFTPE
                     TVASLQAEVGTLTKLVDDLHQLSMSDEGALAYQKSSVDLIPLLEVAGGAFRERFASRG
                     LKLQFSLPDSITVFGDRDRLMQLFNNLLENSLRYTDSGGSLKISAEQHDKTVRLTFAD
                     SAPGVSDDQLQKLFERFYRTEGSRNRASGGSGLGLAICLNIVEAHNGRIIAAHSPFGG
                     VSITVELPLERDLQREV"
     gene            10355..11077
                     /gene="baeR"
                     /locus_tag="PN977_003966"
     CDS             10355..11077
                     /gene="baeR"
                     /locus_tag="PN977_003966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BaeR"
                     /protein_id="HDI6043947.1"
                     /translation="MTELPIDENTPRILIVEDEPKLGQLLIDYLRAASYAPTLISHGD
                     QVLPYVRQTPPDLILLDLMLPGTDGLTLCREIRRFSDIPIVMVTAKIEEIDRLLGLEI
                     GADDYICKPYSPREVVARVKTILRRCKPQRELQQQDAESPLIIDEGRFQASWRGKMLD
                     LTPAEFRLLKTLSHEPGKVFSREQLLNHLYDDYRVVTDRTIDSHIKNLRRKLESLDAE
                     QSFIRAVYGVGYRWEADACRIV"
     gene            11257..11589
                     /locus_tag="PN977_003967"
     CDS             11257..11589
                     /locus_tag="PN977_003967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegP family protein"
                     /protein_id="HDI6043948.1"
                     /translation="MAGWFELSKSSDNQFRFVLKAGNGETILTSELYTSKASAEKGIA
                     SVRSNSPQEERYEKKTASNGKFYFNLKAANHQIIGSSQMYATAQSRETGIASVKANGT
                     SQTVKDNT"
     gene            11798..12094
                     /locus_tag="PN977_003968"
     CDS             11798..12094
                     /locus_tag="PN977_003968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001307279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system ParD family
                     antitoxin"
                     /protein_id="HDI6043949.1"
                     /translation="MKYTDVRFLLTGGRKMARTMTVDLGDELREFIESLIESGDYRTQ
                     SEVIRESLRLLREKQAESRLQALRDMLAEGLSSGEAQPWEKDAFLRKVKAGIRK"
     gene            12096..12392
                     /locus_tag="PN977_003969"
     CDS             12096..12392
                     /locus_tag="PN977_003969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001220181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HDI6043950.1"
                     /translation="MRTIKLMPKANEDLEGIWYYSYHHFGEPQADRYVEHLSDVLQIL
                     SNNNIGTPRPELGEGIFVLPFERHVIYFLQSPEEIIVIRILNQNQYASRHLHWS"
     gene            12495..>12997
                     /locus_tag="PN977_003970"
     CDS             12495..>12997
                     /locus_tag="PN977_003970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HDI6043951.1"
                     /translation="MFKPELLSPAGTLKNMRYAFAYGADAVYAGQPRYSLRVRNNEFN
                     HENLQLGINEAHALGKKFYVVVNIAPHNAKLKTFIRDLKPVVEMGPDALIMSDPGLIM
                     LVREHFPEMPIHLSVQANAVNWATVKFWQQMGLTRVILSRELSLEEIEEIRNQVPDME
                     IEIFVHGA"
BASE COUNT         2869 a         3515 c         3588 g         3025 t
ORIGIN      
        1 gatagtgttt cataattcct ccatttttct cccttattgg ctggctacac tagtatcatt
       61 ccgcgaaacg tttcaggaag agaaactctt aacgatgaaa ggcagttata aatcccgttg
      121 ggtaatcgta atcgtggtgg ttatcgccgc catcgccgca ttctggttct ggcaaggccg
      181 caatgactcc cggagtgcag ccccaggggc gacgaaacaa gcgcagcaat cgccagcggg
      241 tggtcgccgt ggtatgcgtt ccggcccatt agccccggtt caggcggcga ccgccgtaga
      301 acaggctgtt ccgcgttacc tcaccgggct tggcaccatt accgccgcta atactgttac
      361 ggtgcgcagc cgcgtggacg gtcaactgat ggcgttgcat ttccaggaag gccagcaggt
      421 caaagctggc gatttattgg cagaaattga ccccagccag ttcaaagttg cattagcaca
      481 agccctgggc caactggcaa aagataaagc cacgcttgcc aacgcccgcc gtgacctggc
      541 gcgttatcaa caactggtaa aaaccaatct cgtatctcgt caggaactgg atgcccaaca
      601 agcgctggtc agtgaaaccg aaggtaccat taaggctgat gaagcaagcg tcgccagcgc
      661 gcaattgcaa ctcgactgga gccgtatcac cgcaccagtt gatggtcgcg ttggtctcaa
      721 gcaggttgat gttggtaacc aaatctccag tggcgacacc accgggattg tggtgatcac
      781 ccaaacacat cctatcgatc tggtctttac tctgccggaa agcgatatcg ccaccgttgt
      841 acaggcacaa aaagccggaa aaccgctggt ggtagaagcc tgggatcgca ccaactcgaa
      901 gaagttaagt gaaggcacgc tgttaagcct tgataaccaa atcgatgcca ctaccggtac
      961 gattaaagtg aaagctcgct ttaataatca ggatgatgcg cttttcccta atcagtttgt
     1021 taacgcgcgc atgttagtcg acaccgaaca aaacgccgtg gtgatcccca ccgccgctct
     1081 gcaaatgggc aacgaaggcc attttgtctg ggtgctgaat agcgaaaaca aggtcagcaa
     1141 acatctggtg acaccgggca ttcaggacag tcagagagtg gtgatccgcg caggtatttc
     1201 tgcgggcgat cgcgtggtga cggatggcat tgatcgcctg accgaagggg cgaaagtgga
     1261 agtggtggaa gcccagagcg ccaccacttc ggaagagaaa gccaccagcc gcgaatacgc
     1321 gaaaaaagga gcacgctcct gatgcaggtg ttacccccga gcagcacagg cggcccgtcg
     1381 cgcctgttta ttatgcgtcc tgtcgccacc acgctgctga tggtggcgat cttactcgcc
     1441 gggattatcg gttatcgcgc cctgcccgtt tcggcgctgc cggaagtgga ctatccaacc
     1501 attcaggtgg tcaccctcta cccaggtgcg agcccggatg tcatgacctc tgccgttacc
     1561 gcgccgcttg aacgccagtt tgggcagatg tctggcctga aacagatgtc gtcgcaaagt
     1621 tccggcggtg cgtcagttat cactttgcag ttccagctaa cattaccgct cgatgtcgcc
     1681 gagcaggaag tgcaggccgc gattaacgct gcgaccaact tgttgccgag cgatctgcct
     1741 aacccgccgg tttacagcaa agtgaacccg gcagatccgc cgatcatgac gctcgctgtc
     1801 acctcaaccg ccatgccgat gacgcaagtg gaagatatgg tggaaacccg cgtcgcgcaa
     1861 aaaatctcgc agatttccgg cgtcggcctg gtgacgcttt ccggcggcca gcgtccggct
     1921 gttcgcgtca aacttaacgc ccaggcgatt gccgccctcg gcctgaccag cgaaaccgtg
     1981 cgcaccgcta ttaccggcgc taacgttaac tcggcaaaag gtagcctcga cggcccttcc
     2041 cgtgcggtca cactttccgc gaacgaccag atgcaatccg ccgaagagta tcgccagctg
     2101 atcatcgcct accagaacgg cgcgccgatt cgtctgggcg atgtcgcaac cgtagagcaa
     2161 ggagcagaaa acagctggct cggcgcgtgg gcgaacaaag aacaggccat tgtgatgaat
     2221 gttcagcgcc agcccggtgc taacattatc tccaccgccg acagcatccg gcagatgctg
     2281 ccacaactca ccgagagttt gccgaaatcg gtgaaggtga cagtactttc tgaccgtacc
     2341 accaatattc gcgcatccgt cgatgatact cagtttgaat taatgatggc tatcgcgctg
     2401 gtagtcatga ttatctacct gtttttgcgc aatattccgg cgaccattat tcctggcgtc
     2461 gcagtgccgc tgtcgttaat cggcaccttc gcagtcatgg tgtttctcga tttttcgatc
     2521 aataacctga cgctgatggc attaactatc gccaccggat tcgtggtcga tgacgccatc
     2581 gtggtgatcg agaacatttc gcgctatatc gaaaaaggcg aaaaaccgct ggcggcggcg
     2641 ctcaagggcg cgggtgaaat cggttttacc attatctcgc tgaccttctc gctgattgca
     2701 gtgctgatcc cgctactgtt tatgggcgat atcgtcgggc gactgttccg cgaatttgcc
     2761 attactctgg cagtcgcgat tttgatctca gccgtggtgt cgctgaccct gacgccgatg
     2821 atgtgcgcac ggatgctcag ccaggagtca ctgcgtaaac agaaccgctt ctcccgtgcc
     2881 tcggagaaaa tgttcgacag gataatcgcc gcctatggtc gtggactggc gaaagtgctg
     2941 aatcatccgt ggctgacctt aagcgtggcg ctcagcacgc tgctgcttag cgtgctgctg
     3001 tgggtgttca ttccgaaagg tttcttcccg gtgcaggaca acggcattat tcagggcact
     3061 ttgcaggcac cgcaatccag ctcctttgcc aatatggccc agcggcaacg ccaggtcgcg
     3121 gacgtgattt tgcaggatcc ggcagtgcaa agtttgacct catttgttgg cgttgatggc
     3181 actaacccgt cgctgaacag tgcacgttta cagatcaatc tcaaaccact ggatgaacga
     3241 gatgaccggg tgcaaaaagt catcgcccgt ctgcaaacgg cggtagataa agtgccgggc
     3301 gtcgatctct tcctgcaacc aacgcaggac ctgactattg atactcaggt cagccgcacc
     3361 cagtaccagt ttaccttgca ggccacgtca ctggatgcgc tcagtacctg ggtgccacag
     3421 ttgatggaaa aactccagca gctgccacag ctttctgatg tctccagcga ctggcaggac
     3481 aaagggctgg tggcgtatgt caatgttgat cgcgacagcg ccagccgtct ggggatcagc
     3541 atggcggacg tcgataacgc cctttacaac gcgtttggtc agcgactgat ttccactatt
     3601 tatactcagg ccaaccagta tcgcgtggtg ctggagcaca acaccgaaaa tactcctggg
     3661 cttgcggcgc tggatactat tcgcctgacc agcagcgacg gcggcgtggt gccgctaagc
     3721 tcaattgcca aaattgagca gcgttttgcg ccgctttcca tcaaccatct ggatcagttc
     3781 ccggtaacga ccatctcctt taacgtgccg gataactatt cgctgggcga tgcggtgcaa
     3841 gcgattatgg acaccgaaaa gacgctgaat ctgccggtgg atatcaccac gcagttccag
     3901 ggcagcaccc tcgccttcca gtcggcgctg ggcagcacgg tctggctgat tgtcgcggcg
     3961 gtggtggcga tgtatatcgt gctcggcatt ctgtacgaga gctttattca cccgatcacc
     4021 attctctcga cgctacccac ggcaggggtc ggcgcgctgc tggcgttaat gattgccggt
     4081 agcgaactgg atgtgattgc cattatcggc attattttgc tgatcggtat cgtgaagaag
     4141 aacgccatca tgatgatcga cttcgcgctg gctgctgagc gcgagcaagg catgtcgccg
     4201 cgcgaagcta tttaccaggc ttgtctgttg cgttttcgtc cgatcctgat gaccactctg
     4261 gcggctctgc ttggtgcgct gccgctgatg ttgagtaccg gggtcggcgc ggaactacgt
     4321 cgtccgttag gtatcggcat ggtcggcggt ctgatagtca gccaggtgct gacgctgttt
     4381 accacgccgg tgatttattt gctgttcgac cgcctggcat tgtggaccaa aagccgcttt
     4441 gcccgtcatg aagaggaggc gtaagtgaag ttttttgccc tcttcattta ccgcccggtg
     4501 gcgacgattt tactgtcggt cgccattacc ctgtgcggca tactgggctt ccgtatgctg
     4561 ccggtcgccc cgctgccgca ggtcgatttt ccggtgatta tggtcagcgc ctcgctgccc
     4621 ggtgcgtcac cagaaacaat ggcgtcttcc gttgccacgc cgctggagcg ctcacttggg
     4681 cgcattgccg gagtcagcga aatgacctcc agcagttcgc tcggcagcac gcgcattatt
     4741 ttgcagtttg attttgaccg ggatatcaac ggcgcagcgc gcgatgtgca agcggcgatc
     4801 aacgccgcgc aaagtctgct acccagcggg atgcccagcc gtccgaccta tcgcaaagcg
     4861 aacccatcgg atgcgccaat tatgatcctc acgctgacat ccgatactta ttcgcagggt
     4921 gaactgtacg atttcgcctc gacacagctg gctccgacga tttcgcaaat cgacggtgtc
     4981 ggtgatgtcg atgttggcgg cagctcgctg cccgccgtac gcgtcgggct gaatccgcag
     5041 gcgctgttta atcagggcgt ctcgctggac gacgtacgca ctgccatcag caatgccaac
     5101 gtgcgtaaac cgcagggcgt actggaagat ggcacccacc gctggcagat ccagaccaat
     5161 gacgaactga aaactgccgc tgaatatcag ccgctgatta ttcattacaa caacggcgga
     5221 gcggttcgtc tgggcgatgt ggcgacggtg accgactctg tacaagatgt acgtaacgcc
     5281 gggatgacca acgccaaacc ggccattttg ctgatgatcc gcaagctgcc ggaagccaat
     5341 attatccaga cggtagacag catccgcgcc cgtctgccgg aattacaatc caccatcccg
     5401 gcagcaattg atctgcaaat tgcccaggat cgctccccta ctatccgcgc gtcgctggaa
     5461 gaggttgagc agacgctgat tatctcggtg gcgctggtga ttctggtggt gtttttgttc
     5521 ctgcgttcgg gtcgcgccac cattattccc gccgttgcgg tgccggtttc gctgattggt
     5581 acgtttgcgg cgatgtacct gtgcggattc agtctcaata acctttcgtt aatggcactc
     5641 accatcgcta ctggtttcgt ggtggatgac gccatcgtgg tgctggaaaa cattgcccgt
     5701 cacctggaag cggggatgaa accactacag gccgcactgc aaggtactcg cgaagtcggt
     5761 tttacggtgc tgtcgatgag tctgtcactg gtggcggtgt tcctgccgct gctgttgatg
     5821 ggcggattgc cgggccgact gttacgcgag tttgccgtga cgctttctgt cgccattggt
     5881 atttcgttgc tggtttccct gacgctaacg ccgatgatgt gcggctggat gctgaaagcc
     5941 agcaagccgc gcgagcaaaa gcgactgcgt ggttttggtc gcatgttggt agccctgcaa
     6001 caaggctacg gcaagtcact gaaatgggtg ctcaatcata cccgtctggt gggcgtggtg
     6061 ctgcttggca ccattgcttt gaatatctgg ctgtatatct cgatcccgaa aaccttcttc
     6121 ccggagcagg acactggcgt actgatgggc gggatacagg cggatcagag tatttcgttt
     6181 caggcgatgc gcggcaagtt gcaggatttt atgaaaatta tccgtgacga tccggcggtg
     6241 gataatgtca ccggatttac cggcggttcg cgagtgaaca gcgggatgat gtttatcacc
     6301 ctcaagccac gcggcgaacg cagcgaaacg gcgcagcaaa ttatcgaccg tctgcgggta
     6361 aagctggcga aagagccggg ggcgaatctg ttcctgatgg cggtacagga tattcgcgtt
     6421 ggcggacgtc agtcgaacgc cagctaccag tacacgttgt tatccgacga cctggcggca
     6481 ctgcgcgaat gggagccgaa aatccgcaaa aaactggcga cgttgccgga actggcggac
     6541 gtgaactccg atcagcagga taacggcgcg gagatgaatc tggtttacga ccgcgacacc
     6601 atggcacggc tgggaatcga cgtacaggcc gccaacagcc tgttaaataa cgccttcggt
     6661 cagcggcaaa tctcgaccat ttaccagccg atgaaccagt ataaagtggt gatggaagtg
     6721 gatccgcgct atacccagga catcagtgcg ctggaaaaaa tgttcgttat caataacgaa
     6781 ggaaaagcga tcccgctgtc gtatttcgct aaatggcaac cggcgaacgc cccactgtcg
     6841 gtgaatcatc agggattatc ggcggcctcg accatttcgt tcaacctgcc gaccggtaaa
     6901 tcgctctcgg acgccagtgc ggcgatcgat cgcgcaatga cccagcttgg tgtgccttcg
     6961 acggtgcgcg gtagttttgc cggcacggcg caggtgttcc aggagacgat gaactcgcag
     7021 gtgatcctga ttatcgccgc catcgccacg gtgtatatcg tgctgggtat cctttacgag
     7081 agttacgtac atccgctgac gattctctcc accctgccct cggcgggcgt tggagcgctg
     7141 ttggcgctgg agctgttcaa tgccccgttc agcctaatcg ccctgatagg gatcatgcta
     7201 ttaatcggca tcgtgaagaa aaacgccatt atgatggtcg attttgcgct tgaagcccaa
     7261 cggcacggta acctcacgcc gcaggaagct attttccagg cctgtctgct gcgttttcgc
     7321 ccgattatga tgactaccct ggcggcgctg tttggtgcgc tgccgctggt attgtcgggc
     7381 ggcgacggct cggagctgcg gcaacccctg gggattacta ttgtcggcgg actggtaatg
     7441 agccagctcc tgacgctgta taccacgccg gtggtgtatc tctttttcga ccgtctgcgg
     7501 ctgcgttttt cgcgtaaacc taaacaaacg gtaaccgagt aaatgacaga tcttcccgac
     7561 agcacccgct ggcaattgtg gattgtggct ttcggcttct ttatgcagtc gctggacacc
     7621 accatcgtta acaccgcgct tccctcaatg gcgcaaagcc tcggggaaag tccgttgcat
     7681 atgcacatgg tcatcgtatc ttatgtgctg accgtggctg tgatgctgcc cgccagcggc
     7741 tggctggcgg acaaagtcgg cgtgcgcaat attttcttta ccgccatcgt gctgtttact
     7801 ctcggttcac tattttgcgc actttccggc acgctgaacg aactgttgct ggcacgcgcg
     7861 ttacagggcg ttggcggtgc gatgatggtg ccggtcggca ggttgacggt gatgaaaatc
     7921 gtaccgcgcg agcagtatat ggcggcgatg acctttgtca cactgcccgg tcaggtcggt
     7981 ccgctgcttg gcccggcgct cggcggtctg ctggtggagt acgcatcgtg gcactggatc
     8041 tttttgatca acattccggt ggggattatt ggggcgatcg ccacactgat gttaatgccg
     8101 aactacacca tgcagacgcg gcgcttcgat ctctccggct ttttattgct ggcggttggc
     8161 atggcggtat taaccctggc gctggacggc agtaaaggta caggtttatc gccgctggcg
     8221 attgctggcc tggtcgcagt tggcgtggta gcactggtgc tttatctgct gcacgccaga
     8281 aataacaacc gcgctctttt cagtctgaaa ctgttccgta ctcgtacctt ttcgctgggt
     8341 ctggcgggga gttttgccgg acgtatcggt agcggcatgt tgccctttat gacgccggtt
     8401 ttcctgcaaa ttggccttgg cttctcaccg tttcatgccg gactgatgat gatcccgatg
     8461 gtgctcggca gcatggggat gaagcgaatt gtggtacagg tagtgaatcg ctttggttat
     8521 cgtcgggtac tggtggcgac cacgctgggt ctgtcgctgg tcaccctgtt gtttatgacc
     8581 actgctctgc tgggctggta ctacgttttg ccgttcgtcc tgtttttaca agggatggtc
     8641 aactcgacgc gtttctcctc catgaacacc ctgacgctga aagatctccc ggacaatctg
     8701 gcgagcagcg gaaacagcct gctgtcgatg attatgcaat tgtcgatgag tatcggcgtc
     8761 actatcgccg ggctgttgct gggacttttt ggttcacagc atgtcagcgt cgacagcggc
     8821 accacacaaa ccgtctttat gtacacctgg cttagcatgg cgtttatcat cgcccttccg
     8881 gcgttcatct ttgccagagt gccgaacgat acgcatcaaa atgtagctat ttcgcggcga
     8941 aaaaggagcg cgcaatgaag ttctggcgac ccggtattac cggcaaactg tttctggcga
     9001 ttttcgccac ctgcattgtc ttattgatca cgatgcactg ggcggtacgt atcagttttg
     9061 agcgcggctt tatcgattac atcaagcatg gtaatgaaca acggctgcaa atgctcggcg
     9121 atgcgcttgg tgagcagtac gcccagcacg ggaactggcg cttcctgcgt aataacgatc
     9181 gctttgtatt tcagatccta cgttcgctgg agcatgataa caacgaagat aagcccggcc
     9241 ccggtatgcc gccacacggt tggcgcacgc aattctgggt ggttgatcaa aacaacaaag
     9301 tgctggttgg cccgcgagca ccgattccac ccgacggcac acggcggccc attatggtca
     9361 atggggcgga agtgggtgcg gtgatcgcct cccctgttga acgactgacc cgcaataccg
     9421 atatcaattt tgacagacaa cagaggcaaa ccagttggct gattgtcgct ttatctacct
     9481 tgttagcggc gctggcgaca ttcccactgg cgcgcggttt gctggctccg gtcaaacgac
     9541 tggtggacgg tacacacaaa ctggcagcgg gcgatttcac tactcgcgtg acgcccacca
     9601 gtgaagatga attgggcaaa ctggcgcaag acttcaacca gctcgccagc acgctggaga
     9661 aaaaccaaca gatgcgccgc gattttatgg ccgatatctc ccacgagctg cgcacgcctt
     9721 tagcggtact gcgcggcgaa ctggaagcta ttcaggatgg cgtgcgtaaa ttcacgccgg
     9781 agacggtggc ttctttacag gcagaggtcg gtacactgac caaactggtg gatgatcttc
     9841 atcaattgtc gatgtctgat gaaggcgctc tcgcctacca gaaatcgtcg gtggatctga
     9901 tcccgctact ggaagtcgcg ggtggcgcat ttcgtgagcg tttcgccagc cgcgggctga
     9961 aactgcaatt ttccctgcca gacagtatta ccgtatttgg cgatcgcgac cgtttaatgc
    10021 agttattcaa taacttactg gaaaacagcc tgcgctacac tgacagcggc ggtagcctga
    10081 aaatctctgc cgagcagcac gacaaaacgg tgcgcctgac ctttgccgac agcgcgccgg
    10141 gcgtcagtga cgatcagcta caaaaattgt ttgaacgttt ttatcgcacc gaaggctccc
    10201 gcaaccgagc cagcggcggt tccgggctgg ggctggcgat ttgcctgaac attgttgaag
    10261 cacataatgg tcgcattatt gctgcccatt cgccttttgg cggggtaagc attacagtag
    10321 agttaccgct ggaacgggat ttacagagag aagtatgacc gagttaccaa tcgacgaaaa
    10381 cacgccacgt attttgatcg tggaagatga accgaagctg gggcagttac tcattgatta
    10441 tctgcgtgct gcgagctatg cgccgacgct tatcagccac ggcgatcagg tactgccata
    10501 tgtgcgccag acaccaccgg atctgatcct gttggatctg atgctccctg gcaccgatgg
    10561 cctgacgttg tgtcgggaga ttcgtcgttt ttctgacatt ccgatcgtga tggtgacagc
    10621 aaaaatcgaa gagatcgacc gcctgctcgg gctggagatt ggcgcagatg actacatctg
    10681 caaaccgtac agcccacggg aagtggtagc gcgcgtcaaa accattttgc gccgttgcaa
    10741 accgcagcgt gagttgcagc aacaggatgc tgaaagcccg ttgattatcg acgaaggtcg
    10801 ttttcaggct tcatggcgcg gaaaaatgct tgacctgacg cctgcggaat ttcgtctgct
    10861 gaaaacgctc tctcatgaac caggaaaagt gttctcccgc gagcaattgc tcaatcatct
    10921 ttatgacgac taccgcgtag taaccgaccg caccatcgac agccacatta aaaacctgcg
    10981 ccgcaagctg gaatctctcg acgccgaaca gtcatttatc cgcgccgttt acggcgtcgg
    11041 ttaccgctgg gaagccgacg cctgccgcat cgtttaatat ttttacctgc cggagaaggc
    11101 attcacgccg catccggcaa cattatccca tgcattgtcg gctatctgac attatctctc
    11161 cgccctggca agcacctgaa aatcgctcat actttaatcg gcaaacagtc cttttagatg
    11221 ctgttttcga tacgcaattt caatcaagga gtcattatgg ctggttggtt tgaactcagt
    11281 aagagcagtg ataatcaatt ccggtttgtg ctaaaagcgg gcaatggtga gactatcctc
    11341 accagcgagc tgtatacctc aaaagcctct gcggaaaagg gcatcgcgtc ggtacgtagc
    11401 aacagcccgc aagaagaacg ctatgagaaa aaaacggcaa gcaacggcaa attctatttc
    11461 aatctgaaag ccgccaatca tcaaattatc ggctccagcc agatgtacgc caccgcgcaa
    11521 tctcgtgaaa ccggaattgc ctccgttaaa gccaatggca caagccagac ggtaaaagat
    11581 aatacgtaat gacgatgccg ggcgtgggtt gttggcgtcc ggctttatat caaaacaact
    11641 cttatttatt gataattaag attaacctcc gccattaaaa tagtgggcac tgacgagcac
    11701 tcgttgtgct ttctttgttt accatcccat acttatcttc ctgaacatgt tcatatgcca
    11761 aaatatcttc taatactttc gtgacgctat ccggtttatg aaatacactg atgtaagatt
    11821 tttacttaca ggcgggcgta aaatggccag aacgatgacg gttgatctcg gcgatgagtt
    11881 gcgtgaattt atcgagtcct taattgaatc cggcgactat cgcacccaaa gcgaagtgat
    11941 ccgtgagtcg ctgcgcttac ttcgagaaaa gcaagcagag tctcgcctgc aagcgttacg
    12001 tgacatgctc gctgaagggt tgagtagtgg tgaagctcag ccgtgggaaa aggacgcgtt
    12061 tttacggaag gtgaaagcag ggataaggaa ataatgtgcg tactataaaa ctcatgccaa
    12121 aagccaatga ggatttagaa ggcatctggt attacagcta tcaccatttc ggcgaaccac
    12181 aagccgacag atatgtggaa catctttcag acgttctcca gattctgagt aacaataata
    12241 taggtacgcc cagacctgaa ttaggcgaag gtatttttgt tcttcctttt gaacgccacg
    12301 taatttattt cctgcaatca ccagaggaaa ttatcgtcat tcgtatcctt aaccaaaacc
    12361 agtacgcctc cagacattta cactggagct aaacccgccg attgcgctac aatgcccgcc
    12421 cttaaagtgg gggcactccc ctaaccgctt catcaggtga agcggatctg acctgtcatc
    12481 agaacgagag aattatgttt aaaccggaac tcctttcccc ggcgggaacg ctgaaaaata
    12541 tgcgttacgc tttcgcttat ggcgcagatg ctgtttatgc gggccagccg cgttactccc
    12601 tgcgtgtgcg caacaacgaa ttcaaccacg aaaatctcca gctcggcatc aatgaagccc
    12661 acgcgctggg gaaaaagttt tatgtcgtgg tcaacattgc accgcacaac gccaagctga
    12721 aaacctttat ccgtgacctg aaaccggtgg tggagatggg gccggatgcg ctgattatgt
    12781 ccgatccagg gctgattatg ctggtgcgcg agcacttccc tgaaatgccg atccacctct
    12841 cggtgcaggc taacgccgtg aactgggcga cggtgaaatt ctggcagcaa atgggactga
    12901 cccgcgtgat cctctctcgc gagctgtcgc tggaagagat tgaagagatc cgtaatcagg
    12961 tgccggatat ggagatcgag atcttcgttc acggtgc
//
LOCUS       DAMZFT010000130        12955 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.130, whole genome shotgun sequence.
ACCESSION   DAMZFT010000130 DAMZFT010000000
VERSION     DAMZFT010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12955)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12955)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12955
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.130"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            39..722
                     /locus_tag="PN977_003971"
     CDS             39..722
                     /locus_tag="PN977_003971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methylase"
                     /protein_id="HDI6043952.1"
                     /translation="MSRFVLGNCIDVMARIPDNGIDFILTDPPYLVGFRDRQGRTIAG
                     DKTDEWLQPACNEMYRVLKKDALMVSFYGWNRVDRFMAAWKNAGFSVVGHLVFTKNYT
                     SKAAYVGYRHECAYVLAKGRPALPQKPLPDVLGWKYSGNRHHPTEKPVTSLQPLIESF
                     THPNAIVLDPFAGSGSTCVAALQSGRRYIGIELLEQYHRAGQQRLAAVQRAMQQGAAN
                     DDWFMPEAA"
     gene            723..944
                     /locus_tag="PN977_003972"
     CDS             723..944
                     /locus_tag="PN977_003972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6043953.1"
                     /translation="MNYAGHEKLRAEVAEVANAMCDLRTTMNEMERRYSFNADTLPER
                     LVRQTLFRANRLLMEAYTEILELDSCFKD"
     gene            956..1390
                     /locus_tag="PN977_003973"
     CDS             956..1390
                     /locus_tag="PN977_003973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 domain-containing protein"
                     /protein_id="HDI6043954.1"
                     /translation="MYGTCEILCRELAAKYPADTPLMLVVWSPEEIQALADGMDISLS
                     DHEIRTVLARLEDIPEDQRIESGISSGVAMEIISNVRENRQVTVPAELLASLIQTAEQ
                     ALWKREWAARDNGLAVPECVTRRQAVINQARTLLKNNTHENN"
     gene            1441..2211
                     /locus_tag="PN977_003974"
     CDS             1441..2211
                     /locus_tag="PN977_003974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6043955.1"
                     /translation="MNKTLNALVCRHARNLLLAQGWPEETDVDQRNPNYPGWISIYVR
                     LDAPRLATLLINRHGGVLPPHLASAIQRLTGTGAELVLSGSQWQSLPVLPADGTQVSF
                     PYAGEWLTEDEIRAVLDAVHDAVRSICYQVAEDARRIRAALTTTGQTLLTRQTRRFRL
                     VVKESDHPCWLDEDDENLPVVLDAIVNRGARFSSVEMYLVSDCIEHILSCGLACDVLR
                     IPDEPPRRWFDRGVLREVVREARTEIRSMADALAKIRK"
     gene            2752..3054
                     /locus_tag="PN977_003975"
     CDS             2752..3054
                     /locus_tag="PN977_003975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HDI6043956.1"
                     /translation="MMPEYEGGFWHFIRLPDGGGYMMPDGDRFHIVNGANWFDRTVSA
                     DACGIILTSLVINRQLWLYHDSGDAGLTQLYRMRDAQLWRHIEFHPECNAIYAALD"
     gene            3101..3523
                     /locus_tag="PN977_003976"
     CDS             3101..3523
                     /locus_tag="PN977_003976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 family protein"
                     /protein_id="HDI6043957.1"
                     /translation="MYCTVKEIIREVLDTDVPDSECVFAVVLTRGDVRHIAQDWNLSD
                     DELETVMQRLDDAFEHGADVSVVHDVVRELMEEKRVSRQVTVPAVMLEKVVALAGSEM
                     KRLYAVGSENGGDGDAFVREEREAMDVVLQALDGEHMS"
     gene            3520..3711
                     /locus_tag="PN977_003977"
     CDS             3520..3711
                     /locus_tag="PN977_003977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6043958.1"
                     /translation="MNISTETREILRNYKAVINARRREMGQKPLTTAQIVDEICDFVA
                     NQQAVFLGGHYILQGSRNR"
     gene            3674..4026
                     /locus_tag="PN977_003978"
                     /pseudo
     CDS             3674..4026
                     /locus_tag="PN977_003978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001367791.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            5111..7012
                     /gene="ltrA"
                     /locus_tag="PN977_003979"
     CDS             5111..7012
                     /gene="ltrA"
                     /locus_tag="PN977_003979"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000936286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HDI6043959.1"
                     /translation="MLPDELEKRLDGIEDASKKGYPVRNLYKIACEPGLWLQAYVNIR
                     ANAGALTQGVSTDTIDGFSIERAEKLAKTLRSREYVAKPVRRVQIPKKDGKTRPLGIP
                     GGDDKLAQEVARIILERVYEPVFSEHSHGFRPKRSCETALRSIRPVWNGVKWIVDVDI
                     KGFFDNIPHKLLLNTLAEKIKDKNFLKLIEQWLKAGYVDNWKYHRSYSGTPQGGIISP
                     LLANIYLDKLDRFVEQNLIPAYTSGTKRRANPDMNRLAHKIHKLRKKVDGMATDSESE
                     KEAIKKEIERLLEEKRSIPSQVMNDPNFRRMYYVRYADDFVIGVIGSKKDAEHISRQV
                     RNFITTSLGLEVNEAKTRIRHISEGVNFLGYEIRQADAKKLLKQKMQGRHALRRSTTG
                     IVQLFVPDNIAAKFCHQKKYGCYENVKAVHRSSLQNLSEAEIVLTFNAEMRGLANYYS
                     LALDMKYKLSKLYFIWQISLFKTLANKRRSSVNKVAKSLRQNNGDLAITVQAKNGSRK
                     IEVFKLKHVNRNRTTIVDTEPRTAHITTRTTEIMRRLGARICEYCAKTGRCEVHHVRK
                     LKDLKKGRKAGYKPSLWQLMMIARRRKTMILCASCHDKLHSGKLPDLRQEKGRILLQP
                     PVSSGDSAK"
     gene            7128..7652
                     /locus_tag="PN977_003980"
     CDS             7128..7652
                     /locus_tag="PN977_003980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HDI6043960.1"
                     /translation="MLKDHTYPTLPYRLGNDPEVRYIPNGGAVANLQVATSESWRDKQ
                     TGEMREQTEWHRVVLFGKLAEVAGEYLRKGAQVYIEGQLRTRSWEDNGITRYVTEILV
                     KTTGTMQMLGSAPQQNAQAQPKPQQNGQPQSADATKKGGAKTKGRGRKAAQPESQPQP
                     PEGEDYGFSDDIPF"
     gene            7710..7943
                     /locus_tag="PN977_003981"
     CDS             7710..7943
                     /locus_tag="PN977_003981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 domain-containing protein"
                     /protein_id="HDI6043961.1"
                     /translation="MSEYFRILQGLPDGPFTRKQAEAVAAQYRNVFIEDDHGEHFRLV
                     VRNNGTMVWRTWNFEDGAGYWMNHVIRDFGILK"
     gene            8007..9965
                     /locus_tag="PN977_003982"
     CDS             8007..9965
                     /locus_tag="PN977_003982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HDI6043962.1"
                     /translation="MSVTESKTKTERKSSRKPAKTQETVLSALLAQTEEVSVPLVSLI
                     KSPLNVRTVPYSAESVSELADSIKGVGLLQNLVVHALPGDRHGVAAGGRRLAALNMLA
                     ERGIIPADWPVRVKIIPQELATAASMTENGHRRDMHPAEQIAGFRAMAQEGKTPAQIG
                     DLLGYSPRHVQRMLKLADLAPVILDALAEDRITTEHCQALALENDTARQVQVFEAACQ
                     SGWGGKPEVQTIRRLVTESEVAVAGNSKFRFVGADAFSPDELRTDLFSDDEGGYVDCV
                     ALDAALLEKLQAVAEHLREAEGWEWCAGRMEPVGECREDAGTYRCLPEPEAVLTEAED
                     ERLNELMTRYDALENQCEESDLLEAEMKLIDCMAKVRAWTPEMRAGSGVVVSWRYGNV
                     CVQRGVQLRSEDDATDDADRTEQVQEKASVEEISLPLLTKMSSERTLAVQAALMQQPD
                     KSLALLAWTLCLNVFGSGAYSKPAQISLECKHYSLTSDAPSGKEGAAFMAMMAEKARL
                     AALLPEGWSRDMTTFLSLSQEVLLSLLSFCTACSIHGVQTRECGHTSRSPLDTLESAI
                     GFHMRDWWQPTKANFFGHLKKPQIITALNEAGLSGAARDAEKMKKGDAAEHAEFHMKD
                     NRWVPGWMCSPRPQTDATERADNLADAA"
     gene            10020..10454
                     /gene="psiB"
                     /locus_tag="PN977_003983"
     CDS             10020..10454
                     /gene="psiB"
                     /locus_tag="PN977_003983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000845953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugation system SOS inhibitor PsiB"
                     /protein_id="HDI6043963.1"
                     /translation="MNTELTLNALQSMNAQEYEEIRAAGSDMRRNLTHEVMRKVDAPA
                     NWMMNGEYGSEFGGFFPVQVRFTPAHERFHLALCSPGDVSQLWMLVLVNGGGQPFAVV
                     QVQHIFTPAAISHTLALAATLDAQGYSVNDIIHILMAEGGQA"
     gene            10451..11170
                     /locus_tag="PN977_003984"
     CDS             10451..11170
                     /locus_tag="PN977_003984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001584594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid SOS inhibition protein A"
                     /protein_id="HDI6043964.1"
                     /translation="MSARSRALIPLSAEQQAAMQAVAVTEQRRRQGRTLSAWPYATAF
                     FRCLNGSRRISLTDLRFFAPALTKEEFHGNRLLWLAAVDKLIESFGEVCVLPLPSDAG
                     HRLFPSVPFREGERRRQKTTLTEQKYSRQREREAERRELEYQTCFAQAQIDLAFHTPA
                     TVGSWLSRWSGVVEEHDLETIFWGWCGRFPSLSSFDRFFWQEEPLWRLIFEAGEAGRG
                     APVQVRALEQWMIPNKLENVI"
     gene            11167..11763
                     /locus_tag="PN977_003985"
     CDS             11167..11763
                     /locus_tag="PN977_003985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000978013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6043965.1"
                     /translation="MMKSDEKYQVPAWMRPLLPLLCNTGENDPEELLNDTETTASANV
                     VRYVLIVAVRSQVDLLQLLYRKGLLRTEIPGGFSPEEAQELLDNLVRSHISRALSGER
                     MAARDRNADLAWIRQQLVDAAWFVRATLEAHGMSVGNESPPAQPETMPDIQTRELVML
                     IKRLASSLKAVKPDSCVVREAQDWLRDRKLVSITDVLR"
     gene            12228..12728
                     /locus_tag="PN977_003986"
     CDS             12228..12728
                     /locus_tag="PN977_003986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000117633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HDI6043966.1"
                     /translation="MSVVAPAVYVGTWHKYNCGSIAGRWFDLTTFDDERDFFAACRAL
                     HQDETDPELMFQDYEGFPGNMASECHINWAWVEGFRRARDEGCEEAYRLWVDDTGETD
                     FDSFRDAWWGEADSEEAFAVEFVSDTGLLADVPETVALYFDYEAYARDLFLDSFTFID
                     GHVFRR"
BASE COUNT         3025 a         3343 c         3829 g         2758 t
ORIGIN      
        1 gccctcccgg tgccctctga gaaagaagga gtgttgttat gtcccgtttt gtcctcggta
       61 actgcattga tgttatggcg cgtattcctg ataacggcat tgatttcata ctgactgacc
      121 cgccatacct cgtcggtttt cgtgaccgac aggggcgtac catcgccggc gataaaaccg
      181 atgaatggct gcaaccggcc tgtaatgaaa tgtatcgcgt actcaaaaaa gatgcgctga
      241 tggtcagctt ctacggctgg aaccgcgtcg atcgctttat ggccgcctgg aaaaatgcgg
      301 gattcagtgt ggttgggcac ctggtcttca ccaaaaacta cacatcgaag gccgcatatg
      361 tgggttatcg ccacgaatgc gcctacgttc tggcaaaagg ccgtccggcg ctgcctcaga
      421 agccattacc cgacgtgctg ggctggaaat attcaggcaa tcgtcatcac ccgacggaaa
      481 agcctgttac cagcctgcaa ccactgattg agagcttcac acatccgaac gcgattgtgc
      541 tggacccgtt tgcaggcagc ggctcaacct gcgtggctgc ccttcagtcc ggacgccggt
      601 atatcggtat cgagctgctt gagcagtatc accgtgccgg acagcaacgt ctggctgccg
      661 tacagcgggc catgcagcag ggggccgcaa atgatgactg gtttatgccg gaggctgcgt
      721 aaatgaacta tgccggacat gaaaaacttc gcgccgaagt ggcggaggtg gccaatgcca
      781 tgtgcgacct gcgtacaacc atgaatgaga tggagcggcg gtacagcttt aatgctgaca
      841 ccctgccgga acgtctggtg cgtcagacgt tgtttcgcgc aaaccgcctc ctgatggagg
      901 catataccga aattctggaa ctggattcct gcttcaaaga ttgaggggag agagtatgta
      961 cggaacatgc gaaatactct gccgggagct ggcagcaaag tatccggcag acacgccact
     1021 gatgctggtt gtctggtccc ctgaagagat tcaggccctc gctgacggaa tggatatttc
     1081 cctgtccgat catgaaatca ggaccgtcct ggcgcgcctg gaggacatcc cggaagacca
     1141 gcggattgag tccggtattt cttccggcgt ggcgatggag attatcagca acgtgagaga
     1201 aaaccgccag gtgaccgtcc ctgctgaact gctggcgtcc ctgattcaga ccgctgaaca
     1261 ggcactgtgg aaacgtgaat gggccgcccg tgataacggc cttgccgtcc cggaatgtgt
     1321 tacccgccgt caggcagtga ttaatcaggc ccgcaccctg ctgaaaaaca acacacacga
     1381 aaacaactga tgttatcgcc gcctgccctg cgggcggcga tacagggaga acgcttaatc
     1441 atgaacaaaa cgctgaatgc actggtttgt cgtcacgccc gtaacctgct gctggcgcag
     1501 ggctggccgg aagagacgga tgttgaccag cgaaatccga actatccggg ctggatcagc
     1561 atttacgtgc ggctggatgc gccccggctg gcgacgttac ttatcaaccg tcacggcggc
     1621 gtactgccgc cgcacctggc ctccgccatt cagagactga ccggaaccgg ggcggaactg
     1681 gtactgtccg gcagtcagtg gcagtcgctg ccggtacttc cggcagacgg aacgcaggtg
     1741 tctttcccgt atgccggaga atggctgacc gaagacgaaa tcagggctgt tcttgatgcg
     1801 gtgcacgatg cggtacgaag catctgttac caggtggcag aagatgcgcg gcgtatccgt
     1861 gcggcgctga ccaccaccgg tcagacgttg ctgacccgcc agacgcgccg ctttcgcctg
     1921 gtcgtgaagg aaagcgatca cccctgctgg ctcgatgaag atgacgaaaa cctgcccgtg
     1981 gtgctcgatg ccatcgtgaa ccggggagca cgtttttcgt cggtggaaat gtacctggtc
     2041 agcgattgta ttgagcatat cctgtcctgt gggctggcct gcgatgtgct gcgtataccc
     2101 gatgaaccgc cccgccgctg gtttgaccgt ggtgttctgc gggaggtggt ccgggaagcc
     2161 cggaccgaaa tccgcagcat ggcggatgcc ctggcaaaaa tccggaaatg atgacactgg
     2221 cggggtaaat ccccgccttt ttcctgccgg tccccggaca gagcacaaag gaagtcgccg
     2281 ccggctccgc tttacccggc catgcggggc atggccttgt gggttttcag ttatgtggcc
     2341 accgtgtcgt gtgcgggctg tgccgtgcct ccatcttagc cgggctggca gggatgcaag
     2401 ggtacgcttc gccgctgcgg tcacccggtc cctccttccc gtctgccgtg attttccggt
     2461 cgttcacccg ctcaggtttc gcggtctcac cctccaactc ccgccagccg tgtgcgtcag
     2521 gctgcggctt cccttgcgcc ctgtgcatcc ccgccttatc gcccggcttt tatggaggca
     2581 cggcaccgcc cggtgtcgcg gaatatgtga acaacggagg aatacatcat gcaatatgcg
     2641 aaacctgtca ctctgaatgt tgaagagtgc gaccgtctgt tttttctgcc ttacctgttt
     2701 ggccaggatt ttctgtatgt cgaagcgtct gtgtacgcgc tggcgaaaaa aatgatgccg
     2761 gaatatgaag gcggattctg gcacttcatc cgcctgccgg acggtggcgg ctacatgatg
     2821 ccggatggcg accgtttcca catagtgaac ggtgcaaact ggtttgaccg taccgtgagt
     2881 gctgatgcct gcggcatcat tctgacttcc cttgtgatta accgccagtt gtggctgtac
     2941 cacgacagcg gtgatgcagg actgacccag ctttaccgga tgcgcgatgc gcagttgtgg
     3001 cgtcacatcg aatttcaccc tgaatgcaac gcgatttacg cggctctgga ctgattaacg
     3061 gacggggcgg caatgccgcc cctgtaaagg agacgagaaa atgtactgta ctgttaaaga
     3121 aattatccgc gaagtgctgg atacagacgt gccggacagt gaatgcgttt ttgccgtggt
     3181 gctgacccgt ggggatgtgc gtcacatagc ccaggactgg aacctgtcag acgatgagct
     3241 ggaaaccgtc atgcagcggc tggacgatgc ctttgagcat ggtgcggatg tcagcgttgt
     3301 tcacgacgtt gttcgtgaac tgatggaaga aaagcgcgtc agccgtcagg tgacagtccc
     3361 ggcggtgatg ctggaaaaag tggtggcgct ggcaggcagt gaaatgaagc gcctgtatgc
     3421 cgtcgggagt gagaacgggg gcgacggtga cgcattcgtc agggaagaac gcgaagcgat
     3481 ggacgttgtg ttacaggcgc tggacgggga gcacatgtca tgaatatcag cacagaaacc
     3541 cgcgaaattc tgcgcaatta caaagccgtg attaatgcgc ggcgtcgtga aatggggcag
     3601 aaaccgctca ccactgcgca gattgttgat gaaatctgcg attttgtggc gaatcagcag
     3661 gcggttttcc tcggtggtca ctatatcctt cagggcagca gaaacaggtg atacagggca
     3721 ggcggtggag gccgcctgtc agcccgcgcg aaaacccctg gcaggctggc gcctgccgga
     3781 agggatgcgg gccacgctgg cgcgtcgccc gttacccttc ctgttggtga caggcgttgt
     3841 ttcaggaagt acctgttaca gacctgtcgt taaagaacct tcttacacat gcgccccttc
     3901 ggctcccggc tgcctggcgg catccggtcg tcggcccgac tacgacaggc agctgctggc
     3961 gcacccgcca gtctccgccg aacccgctgg cgcggtttcg ggctgccgct acggtccgat
     4021 gcgtgaaaac ccggcttgca gcgaccgttc cggtcgcgcc gcccctgtcg cgccggaaac
     4081 ggcgctccct gcacacctga cggtgtgtaa ggccactgcg cctgatggcg cagtcaaccc
     4141 ttccgccgcc acccctgcgg cctgtttccc gctgtggact ttccgggggg cagacgtaac
     4201 cccctcccgt tccatctgtc cagggtgaga tgcaccggcc acaaaatttt tagccatgaa
     4261 cccggctaaa aattttgcgt ccgcccccgg acagctgtcc cgtgaggcgg taaacgggcc
     4321 cccatcggaa aggtccactg cggggagaaa cccctgtgaa gtggacggca ggaggagctt
     4381 tcgctcctct gaaggggcac atgaacgtcc cgtcgccggt atccggttta tctcacatct
     4441 ttcagtcagg aggttcttat gagtgcacgt ggtatcaaca aggtcatcct cgtcggggtg
     4501 cgccgttgaa gcgcggtgtt ttatggagtg ttgcctgaaa agcaacatgg cttagtggta
     4561 tagccattgc atactgtggt tagccttgct tggtaacagg ctcggagatc ctgaaaagga
     4621 gaggtgaaca tagcatccac agaaaagggt ggtgaatatg gaaatcgtta taagtatcac
     4681 cattcaatgc aagatgtata tggataaact gggtttgttg aagtcgagat ctatgtggga
     4741 aacaggaaac caataacgca gaacgattga agcgttattc ccgtactagc acaagaaatc
     4801 cgtccgacgt tgattgtgac cactctaacg ggccagctat tgatgaaata gttaaacgat
     4861 cgaaagacat cctaagtaca tcgcatctcc acaccgtgaa actacgggac tacctaaact
     4921 ggatttatac ccagcatggt agcggagtct ccatagtact caaggtcggc ctgtaatggg
     4981 ttccggcagt ccgcattata cggaagcagc aaaggtataa atcaaccgga aggaagatga
     5041 aagcctcaca gtgctgcggg gaagggagac agttgttaga tacatcaatc tctaagagga
     5101 gggttagcca atgctgcctg atgaacttga gaaaagatta gatggaattg aggatgcttc
     5161 aaaaaaaggg tatccagtac gtaatctata caaaattgcg tgtgagcccg gactgtggct
     5221 gcaagcctac gtgaatatac gggccaatgc aggtgcactg actcaaggtg tatcaacaga
     5281 taccattgat ggcttttcta tcgaaagagc agagaagctg gcaaaaacat tgcggagtag
     5341 agaatacgta gcgaaacctg tacgcagagt tcagatccca aaaaaggacg gaaaaaccag
     5401 accgctgggg attccaggtg gagacgataa actggcgcaa gaagtagcaa gaattattct
     5461 ggaaagggtt tatgaaccag tattctctga acactctcat ggattcagac caaaacgttc
     5521 gtgtgaaact gcgctcagat ctattcgtcc agtctggaac ggagttaaat ggatcgtgga
     5581 tgttgatatc aaaggtttct ttgacaatat cccccacaaa ctattgctga atacactggc
     5641 agagaaaatt aaggataaga atttcctgaa attaatcgaa cagtggctga aggcaggata
     5701 tgtggacaat tggaaatacc atcgctcata ttctggcacg ccacaaggtg ggataatttc
     5761 tcccttactg gcaaatatat acctcgacaa actggaccgt tttgtagaac agaatttaat
     5821 tcctgcatat acaagcggta caaaacgaag agcgaatcca gatatgaaca ggctggccca
     5881 taagattcat aaactacgaa agaaagtaga cggtatggca actgattctg aatcagaaaa
     5941 agaagccata aagaaagaaa ttgaacgact gcttgaagaa aaacgttcta taccatctca
     6001 ggtcatgaac gatccaaact tcaggcgtat gtactatgtt cgatatgctg atgacttcgt
     6061 aatcggtgtc atcggctcga agaaagatgc tgaacatata tcaagacagg tcagaaactt
     6121 tattactact tcactgggat tggaagttaa tgaagcaaag accaggatcc gccatatctc
     6181 agaaggagtt aattttttag gctatgaaat cagacaggca gatgcaaaga aattgctgaa
     6241 gcagaaaatg caagggcggc acgctctccg gagatccacc acaggaattg tgcagctctt
     6301 cgttcctgac aatatagccg ctaaattttg tcaccaaaag aaatatggct gttatgagaa
     6361 tgtgaaggct gtacatcgtt caagtctaca aaatctcagt gaagctgaaa ttgttctgac
     6421 tttcaacgcg gaaatgcgcg gattggcgaa ctactacagt ctggcgctgg acatgaaata
     6481 taaattatca aaattatatt tcatatggca aataagcctt tttaagaccc tggcgaataa
     6541 acgacgtagt tcagtgaata aagtcgccaa atctctacgg caaaataatg gagatctggc
     6601 tattaccgtt caggccaaaa atggatcccg aaagattgag gttttcaaac tcaaacatgt
     6661 caatcgcaac cgaacaacga ttgtcgatac ggaaccgaga accgcacata tcacaacccg
     6721 cacaacagag atcatgcgtc gattaggggc aaggatctgc gagtattgtg cgaagactgg
     6781 gcgctgcgaa gtacatcatg tcagaaagct aaaagacctg aagaaaggtc gtaaggccgg
     6841 atataaaccc agcttgtggc aactaatgat gatcgcacgc cgccggaaaa cgatgatttt
     6901 gtgcgcaagt tgccacgaca agttacatag cggaaaacta cccgatttga gacaggaaaa
     6961 gggaagaata ctcctgcaac cacctgtctc aagcggggat tctgctaaat gagtttatcc
     7021 ccaataagct agcaatggag agccgtatgc actgaaaggt gcacgtacgg ttcggagggg
     7081 gggtaacggt gatctaactc aagataagca ggacatattc gccatttatc ttgaaagacc
     7141 acacgtaccc taccctaccc taccgtctgg gcaatgatcc ggaagtccgt tacatcccca
     7201 acgggggcgc agtggcaaac ctgcaggtgg ccacgtcaga aagctggcgc gacaaacaga
     7261 cgggggagat gcgggagcag acggaatggc accgcgtggt gctgttcggc aagctcgcgg
     7321 aagtggcagg tgaatatctg cgcaagggcg cgcaggtcta catcgagggg caactccgta
     7381 cccgtagctg ggaagataac ggtatcaccc gttacgtcac tgaaattctt gttaagacca
     7441 cgggcaccat gcagatgctg gggagtgcac cacagcagaa cgctcaggcg caaccgaagc
     7501 ctcagcagaa tgggcagcca cagagtgctg acgcgacgaa aaaaggtggc gcgaaaacga
     7561 aaggccgtgg acgtaaggcc gcgcagcccg agtctcagcc gcaaccgccg gagggggagg
     7621 attacgggtt ttcagacgat atcccgttct gactggcctg actgtgataa ccgccccgcc
     7681 ctgcgcgggg catcaccgga gatatgagga tgagcgaata tttcagaata cttcagggac
     7741 tgcctgacgg tccttttacc cgcaaacagg ccgaagccgt tgccgcacag taccggaacg
     7801 tctttatcga ggatgaccac ggagaacatt ttcgcctggt tgtccgtaat aacggaacaa
     7861 tggtctggcg tacctggaat tttgaggacg gtgccgggta ctggatgaac catgtcatcc
     7921 gggatttcgg cattcttaag taagagaggt gccggacggg aacgcgtccg gcagacatac
     7981 aaccatcccg gtcaaaggag aaatatatgt cagttacaga gtctaagaca aaaacggagc
     8041 gtaaatccag ccgtaaacct gcaaaaacgc aggaaacagt cctgtctgcc ctgctggcgc
     8101 agacggagga agtgagcgtg ccgctggtct cactgattaa gtcaccgctg aatgtgcgca
     8161 cggtgccgta ttctgcggag tccgtcagtg aactggctga ttccattaag ggggtcggcc
     8221 tgctgcagaa tctggttgtt catgccctgc caggtgaccg ccacggtgtc gccgcaggtg
     8281 gtcgccgact ggcagcactc aacatgctgg cagagcgtgg aatcattccg gctgactggc
     8341 ctgtccgcgt gaaaattatt ccgcaggaac tggcgacggc ggcatcaatg actgaaaacg
     8401 gtcatcgtcg ggatatgcac cctgccgaac agattgccgg attccgtgca atggcgcagg
     8461 aaggcaaaac acctgcacaa atcggtgatt tgctgggcta ttcaccccgc cacgttcagc
     8521 gaatgctgaa actggcagac cttgcgcctg tcattctcga tgcgctggca gaggaccgca
     8581 tcaccacaga gcactgtcag gcgctggcgc tggagaacga caccgcgcgt caggtgcagg
     8641 tgtttgaagc cgcctgtcag tcaggatggg gcggtaaacc ggaagtgcag accattcgtc
     8701 gtctggtgac cgaaagtgaa gtggcggtgg cggggaacag taaattccgc ttcgtggggg
     8761 ctgatgcctt ctcgccagat gaactgcgca ccgatttgtt cagcgacgac gagggtggct
     8821 atgtggactg cgtggcgctc gatgccgccc tgctggaaaa actccaggct gttgctgaac
     8881 accttcggga agccgaaggc tgggaatggt gcgccggacg catggagcct gtcggtgagt
     8941 gccgtgagga tgccggaaca taccgctgtc tgccggagcc ggaagcggtg ctgacggagg
     9001 cggaagacga acgcctgaac gaactgatga cgcgttacga cgcgctggaa aaccagtgtg
     9061 aggaatccga cctgctggaa gcagaaatga agctgattga ctgcatggcg aaggtcaggg
     9121 cgtggacgcc ggagatgcgt gccggaagtg gcgtggtggt gtcctggcgt tatggaaacg
     9181 tatgtgtcca gcgtggtgtg cagttgcgca gtgaagatga cgcgactgat gacgctgacc
     9241 gcacggaaca ggtgcaggag aaagcgtcag tggaggaaat cagtctgccg ttgctgacga
     9301 aaatgtcttc cgaacgcacg ctggcagtcc aggcggcact gatgcagcag ccggacaaat
     9361 ccctggcact gctggcatgg acgctctgcc tgaatgtgtt tggcagcgga gcgtacagta
     9421 aaccagcaca aatcagcctg gaatgtaaac attattcgct gaccagcgat gcgccatcgg
     9481 ggaaggaagg tgccgcgttc atggcgatga tggcagaaaa agcccgtctt gcggccctgt
     9541 taccggaggg atggtcacgg gacatgacga cgttcctttc actcagtcag gaggtgctgt
     9601 tatccctgct cagtttctgc accgcatgca gcatccacgg cgttcagacc cgcgagtgtg
     9661 gtcacacgtc acgcagcccg ctggacacgc tggagagcgc catcggtttt cacatgcgcg
     9721 actggtggca gccgacaaaa gcaaacttct tcggacacct gaaaaagccg cagattatca
     9781 cagccctgaa tgaggcagga ctgtccggtg ccgcacggga cgcagagaag atgaagaaag
     9841 gcgatgccgc tgaacatgca gagttccata tgaaagacaa ccgctgggtg cctggctgga
     9901 tgtgttcacc acgtccacag acggatgcca ctgaacgcgc cgataacctg gctgatgccg
     9961 cctgatgaac aaccacaccg ccccgccgga gacggggcgg cagagggaga aatacgatga
    10021 tgaataccga actgacactg aatgccctgc agtccatgaa tgcccaggaa tatgaagaaa
    10081 tccgtgctgc gggaagcgat atgcgccgta atctcactca cgaggtgatg cgtaaagtgg
    10141 acgcaccggc taactggatg atgaacggag agtatggcag cgagttcggg ggctttttcc
    10201 ccgtccaggt tcgtttcacg ccagcccacg aacgtttcca cctggcatta tgttcgccgg
    10261 gagacgtctc tcagctctgg atgctggttc tggtgaatgg tggtggacag cctttcgccg
    10321 tcgttcaggt gcaacatatc ttcacgcctg ccgcgatcag tcacacgctg gcgcttgccg
    10381 cgacactgga tgcgcaggga tacagtgtta acgacatcat ccatatcctg atggcagaag
    10441 gaggtcaggc atgagcgcac gttcacgggc actgatcccc ctcagcgcag aacaacaggc
    10501 cgccatgcag gcggtggctg tcacagaaca acgtcgtcgt cagggacgca cactttcagc
    10561 atggccttat gccaccgctt tctttcgctg cctgaatggc agtcgccgga tttcgctgac
    10621 cgatctccgc ttttttgccc ctgcgctgac gaaggaggaa tttcatggca accgtctcct
    10681 gtggctggct gccgtggata aactgattga aagttttggg gaagtctgtg ttcttcccct
    10741 gccatccgat gcggggcacc gtctgttccc gtccgtgcct tttcgtgaag gtgagcgtcg
    10801 tcgtcagaaa accacgctga cagagcagaa atacagccgc cagcgggaac gtgaggcaga
    10861 acgacgggaa ctggaatacc agacatgttt tgctcaggcg cagattgacc ttgcgtttca
    10921 tactcccgcc acggtcggaa gctggttgtc ccgctggtct ggtgttgttg aggagcatga
    10981 tctggaaacg attttctggg ggtggtgcgg gcgttttcca tcactgtcat catttgaccg
    11041 gtttttctgg caggaggaac cactctggcg gctgattttt gaagccggtg aggccggtcg
    11101 tggtgcaccg gtacaggtac gtgcacttga gcagtggatg atcccgaaca agctggagaa
    11161 cgtaatatga tgaaatcaga cgaaaaatac caggttcccg cgtggatgcg acctctgttg
    11221 ccgttgctct gcaacaccgg ggagaacgat ccggaagaac tgctgaatga tacagaaacc
    11281 actgccagtg cgaatgttgt ccgttatgtg ctgatagttg ctgtgcggtc gcaggttgat
    11341 ctgctgcagc ttctgtacag gaaagggctg ttgcgcacag agataccagg tggcttttca
    11401 ccggaagaag cgcaggaact cctggataat ctggtgcgca gccatatcag cagggcgctg
    11461 tcgggtgagc gaatggcagc ccgtgacagg aatgccgatc tggcctggat tcgacagcaa
    11521 ctggtcgatg ccgcctggtt tgtccgtgcc acactggaag cgcatgggat gagcgtcgga
    11581 aatgagagtc cccctgctca gccggagaca atgccggaca tacagacacg ggaactggtg
    11641 atgttgatca agcgactggc atcatcgctg aaagcggtga aaccggacag ttgtgtggtg
    11701 cgtgaagcgc aggactggct gcgcgacaga aaacttgtga gcatcacgga tgttcttcgg
    11761 tgaaccagtg gaggtatgct tgaggagcag ggacatgccg gtcatgatcg tttcgttcca
    11821 ggggtggtct ggagatcatg cggcgtgtcc tctgcactcg ccggaataag gacgtcgccg
    11881 ccggctccgc ttttacccgg ccatgcgggg catggccttg tgggttttca gctctgtggc
    11941 ctcagcgtcg tgtgcgggct gtgccgtgcc tccatcgtag ccgggcttct gcgggcggga
    12001 tgcaaggtaa gatgaaccgc tgcggtcacc cggtccctcc ttcccgtctg ccgtgatttt
    12061 ccggtcgttc acccgctcag atttcgcggt ctcaccctcc aactcccgtc agccgtgcgc
    12121 gtcgggctgc ggctttcctt gcgcccttgc atccctgcag gcccggcaaa gatggaggca
    12181 cggcaccgcc cggtgtcgca gaacatgtga ctatggagga ttcgggaatg tctgttgttg
    12241 cacctgctgt atacgttgga acctggcaca aatataactg cggaagcatc gccggccgct
    12301 ggtttgacct gaccacgttt gatgatgaac gcgatttttt cgccgcctgc cgtgcccttc
    12361 accaggatga aaccgatccg gaactgatgt ttcaggatta tgagggtttc ccggggaata
    12421 tggcctctga atgtcatatc aactgggcct gggttgaagg cttccgccgg gcacgggatg
    12481 aaggctgcga agaggcttat cgtctctggg tggatgatac cggtgagacg gattttgaca
    12541 gcttccgcga tgcctggtgg ggcgaggctg acagtgagga ggcttttgcg gttgagttcg
    12601 tcagtgatac cggcctgctg gctgacgtgc cggagacggt ggcgctctat tttgactatg
    12661 aggcgtatgc gcgggattta ttcctggact ccttcacctt tattgacggt catgtgttcc
    12721 gtcggtgatt tactcccccg ctccggcggg ggtatggcct gccgggccgc cggaaaaccc
    12781 tggcaggctt acgcctgcct gaaatacagg agggccgcgc ctgcggcgct gcccttcctg
    12841 cccgtcatgg tgtgaggcat ttttctgttt tgtcgtttct gttgttctga tgcccggagg
    12901 cggggacacc cttcggctcc cggctgcctg gcggcatccg gtcgtcggcc cgact
//
LOCUS       DAMZFT010000131        12896 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.131, whole genome shotgun sequence.
ACCESSION   DAMZFT010000131 DAMZFT010000000
VERSION     DAMZFT010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12896)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12896)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12896
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.131"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..1132
                     /gene="puuA"
                     /locus_tag="PN977_003987"
     CDS             <1..1132
                     /gene="puuA"
                     /locus_tag="PN977_003987"
                     /EC_number="6.3.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415813.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glutamate-putrescine ligase"
                     /protein_id="HDI6043967.1"
                     /translation="DRTCVPVLGSLTPSAADPEFIGQMLLTMVDEDGAPFDVEPRNVL
                     NRLWQQLRQRGLFPVVAVELEFYLLDRQRDAEGYLQPPCAPGTDDRNTQSQVYSVDNL
                     NHFADVLNDIDELAQLQLIPADGAVAEASPGQFEINLYHTDNVLEACDDALALKRLVR
                     LMAEKHKMHATFMAKPYEEHAGSGMHIHISMQNNRGENVLSDAEGEDSPLLKKMLAGM
                     IDLMPSSMALLAPNVNSYRRFQPGMYVPTQASWGHNNRTVALRIPCGDRHNHRVEYRV
                     AGADANPYLVMAAIFAGILHGLDNELPLQEEVEGNGLEQEGLPFPIRQSDALGEFIEN
                     DHLRRYLGERFCHVYHACKNDELLQFERLITETEIEWMLKNA"
     gene            1281..1445
                     /locus_tag="PN977_003988"
     CDS             1281..1445
                     /locus_tag="PN977_003988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6043968.1"
                     /translation="MPMIKSPHGEGGCVCAPPATDWTPPPLLPLLNRFDFRSTRPQTL
                     LRRGGSNYGY"
     gene            1435..2820
                     /gene="puuP"
                     /locus_tag="PN977_003989"
     CDS             1435..2820
                     /gene="puuP"
                     /locus_tag="PN977_003989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415812.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PuuP"
                     /protein_id="HDI6043969.1"
                     /translation="MAINSPLTIAAQSGKTRLRKSLKLWQVVMMGLAYLTPMTVFDTF
                     GIVSGISDGHVPASYLLALAGVLFTAISYGKLVRQFPEAGSAYTYAQKSINPHVGFMV
                     GWSSLLDYLFLPMINVLLAKIYLSALFPEVPPWVWVVTFVAILTAANLKSVNLVANFN
                     TLFVLVQISIMVVFIFLVVQGLHKGEGVGTVWSLQPFISENAHLIPIITGATIVCFSF
                     LGFDAVTTLSEETPDAARVIPKAIFLTAVYGGVIFIAASFFMQLFFPDISRFKDPDAA
                     LPEIALYVGGKLFQSIFLCTTFVNTLASGLASHASVSRLLYVMGRDNVFPERVFGYVH
                     PKWRTPALNVIMVGIVALSALFFDLVTATALINFGALVAFTFVNLSVFNHFWRRKGMN
                     KSWKDHFHYLLMPLVGALTVGVLWVNLESTSLTLGLVWASLGGAYLWYLIRRYRKVPL
                     YDGDRTPVSET"
     gene            2954..3199
                     /locus_tag="PN977_003990"
     CDS             2954..3199
                     /locus_tag="PN977_003990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmjA family protein"
                     /protein_id="HDI6043970.1"
                     /translation="MNHDIPLKYFDIADEYATECAEPVADAERTPLALYFQLLLTRLM
                     NNEEISEEAQHEMAAEAGINPVRIDEIAEFLNQWGNE"
     gene            3512..5155
                     /gene="sapA"
                     /locus_tag="PN977_003991"
     CDS             3512..5155
                     /gene="sapA"
                     /locus_tag="PN977_003991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein SapA"
                     /protein_id="HDI6043971.1"
                     /translation="MRQVLSSLLVIAGLVSGQAIAAPESPPHADIRDSGFVYCVSGQV
                     NTFNPSKASSGLIVDTLAAQFYDRLLDVDPYTYRLMPELAESWEVLDNGATYRFHLRR
                     DVPFQKTDWFTPTRKMNADDVVFTFQRIFDRNNPWHNVNGSNFPYFDSLQFADNVKSV
                     RKLDNHTVEFRLAQPDASFLWHLATHYASVMSAEYAGKLEKEDRQEQLDRQPVGTGPY
                     QLSEYRAGQYIRLQRHDDFWRGKPLMPQVVVDLGSGGTGRLSKLLTGECDVLAWPAAS
                     QLSILRDDPRLRLTLRPGMNVAYLAFNTAKPPLNNPAVRHALALAINNQRLMQSIYYG
                     TAETAASILPRASWAYDNEAKITEYNPAKSREQLKALGLENLTLKLWVPTRSQAWNPS
                     PLKTAELIQADMAQVGVKVVIVPVEGRFQEARLMDMSHDLTLSGWATDSNDPDSFFRP
                     LLSCAAIHSQTNLAHWCDPKFDSVLRKALSSQQLAARIEAYDEAQSILAQELPILPLA
                     SSLRLQAYRYDIKGLVLSPFGNASFAGVYREKQDEVKKP"
     gene            5152..6117
                     /gene="sapB"
                     /locus_tag="PN977_003992"
     CDS             5152..6117
                     /gene="sapB"
                     /locus_tag="PN977_003992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease SapB"
                     /protein_id="HDI6043972.1"
                     /translation="MIIFTLRRILLLIVTLFLLTFVGFSLSYFTPHAPLQGASLWNAW
                     VFWFNGLIHWDFGVSSINGQPIAEQLKEVFPATMELCILAFGFALIVGIPMGMIAGIT
                     RHKWQDNLINAIALLGFSIPVFWLALLLTLFCSLTLGWLPVSGRFDLLYEVKPITGFA
                     LIDAWLSDSPWRDEMVMSAIRHMILPVITLSVAPTTEVIRLMRISTIEVYDQNYVKAA
                     ATRGLSRFTILRRHVLHNALPPVIPRLGLQFSTMLTLAMITEMVFSWPGLGRWLINAI
                     RQQDYAAISAGVMVCGSLVIIVNVISDILGAMANPLKHKEWYALR"
     gene            6104..6994
                     /gene="sapC"
                     /locus_tag="PN977_003993"
     CDS             6104..6994
                     /gene="sapC"
                     /locus_tag="PN977_003993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease SapC"
                     /protein_id="HDI6043973.1"
                     /translation="MPYDSVYSEKRPPGTLRTAWRKFYSDASAMVGLYGCAGLAVLCI
                     FGGWFAPYGIDQQFLGYQLLPPSWSRYGEVSFFLGTDDLGRDVLSRLLSGAAPTVGGA
                     FVVTLAATICGLVLGTFAGATHGLRSAVLNHILDTLLAIPSLLLAIIVVAFAGPSLSH
                     AMFAVWLALLPRMVRSIYSMVHDELEKEYVIAARLDGASTLNILWFAVMPNITAGLVT
                     EITRALSMAILDIAALGFLDLGAQLPSPEWGAMLGDALELIYVAPWTVMLPGAAIMIS
                     VLLVNLLGDGVRRAIIAGVE"
     gene            6994..7986
                     /gene="sapD"
                     /locus_tag="PN977_003994"
     CDS             6994..7986
                     /gene="sapD"
                     /locus_tag="PN977_003994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapD"
                     /protein_id="HDI6043974.1"
                     /translation="MPLLDIRNLTIEFKTGDEWVKAVDRVSMTLTEGEIRGLVGESGS
                     GKSLIAKAICGVNKDNWRVTADRMRFDDIDLLRLSARERRKLVGHNVSMIFQEPQSCL
                     DPSERVGRQLMQNIPAWTYKGRWWQRFGWRKRRAIELLHRVGIKDHKDAMRSFPYELT
                     EGECQKVMIAIALANQPRLLIADEPTNSMEPTTQAQIFRLLTRLNQNSNTTILLISHD
                     LQMLSQWADKINVLYCGQTVETAPSKELVTMPHHPYTQALIRAIPDFGSAMPHKSRLN
                     TLPGAIPLLEQLPIGCRLGPRCPYAQRECIVTPRLTGAKNHLYACHFPLNMEKE"
     gene            7988..8794
                     /gene="sapF"
                     /locus_tag="PN977_003995"
     CDS             7988..8794
                     /gene="sapF"
                     /locus_tag="PN977_003995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapF"
                     /protein_id="HDI6043975.1"
                     /translation="MIETLLEVRNLSKTFRYRTGWFRRQTVEAVKPLSFTLREGQTLA
                     IIGENGSGKSTLAKMLAGMIEPTSGELLIDDHPLHFGDYSFRSQRIRMIFQDPSTSLN
                     PRQRISQILDFPLRLNTDLEPEQRRKQIIETMRMVGLLPDHVSYYPHMLAPGQKQRLG
                     LARALILRPKVIIADEALASLDMSMRSQLINLMLELQEKQGISYIYVTQHIGMMKHIS
                     DQVLVMHQGEVVERGSTADVLASPLHELTKRLIAGHFGEALTADAWRKDR"
     gene            complement(8843..10006)
                     /locus_tag="PN977_003996"
     CDS             complement(8843..10006)
                     /locus_tag="PN977_003996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug effflux MFS transporter"
                     /protein_id="HDI6043976.1"
                     /translation="MARVSLSWALILGLLAGIGPMCTDLYLPALPEMSEQLAATTTIT
                     QLTLTASLIGLGVGQLLFGPLSDKIGRKRPLILSLLLFIVSSILCATTNNIYWLVVWR
                     FIQGIAGAGGSVLSRSIARDKYQGVTLTQFFALLMTVNGLAPVLSPVLGGYIVSTFDW
                     RTLFWVMAEISTVLLLGCLLFINETLPENKRGSSLLLTGRSVVQNRRFMRFCLIQSFM
                     LAGLFAYIGSSSFVLQKEFGFSPMQFSLVFGLNGIGLIIASWIFSRLARRINAMTLLR
                     GGLIAAILCALLTVLCAWTQLPIPALVALFFTIAFCSGIGTVGGAEAMSAVGTQESGT
                     ASALMGMSMFVFGGIAAPLSGIGGETLLKMSLAITVCYTLALLVALTRIDNQK"
     gene            complement(10015..11415)
                     /locus_tag="PN977_003997"
     CDS             complement(10015..11415)
                     /locus_tag="PN977_003997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HDI6043977.1"
                     /translation="MLRRSLIFLVLLSAGCVSLDPHYSTPESPIPATLPGAQGQGKAI
                     SHDWQQVIHDPRLQQVIHDPRLQQVVTIALNSNRDVQKAIADIDSARALYGQTNASLF
                     PTVNAALSSTRSRSLANGTGTTAEADGTVSSYTLDLFGRNQSLSRAARETWLASEFTA
                     QNTRLTLIAEISTAWLTLAADNSNLALAKETMASAENSLKIIQRQQQVGTAAATDVSE
                     AMSVYQQARASVASYQTQVMQDKNALNLLAGTTLAENLLPGTLESLPEQMISLVPAGV
                     SSDVLLRRPDIQEAEHNLKSANADIGAARANFFPTISLTASAGVGSDALSSLFSHGMQ
                     IWSFAPSVTLPLFTGGSNLAQLRYAEAQKRGLIATYEKTVQSAFKDVANALARRTTLE
                     EQLDAQRQYVKAEQQTVDVGLRRYQAGVGDYLTVLTAQRSLWSAQQELLALQLTDFTN
                     RITLWQSLGGGMSSLK"
     gene            complement(11419..>12896)
                     /locus_tag="PN977_003998"
     CDS             complement(11419..>12896)
                     /locus_tag="PN977_003998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HDI6043978.1"
                     /translation="GGGMALMMWKLPGSFLPTEDQGEIMVQYTLPAGATAARTAEVNR
                     QIVDWFLINEKANTDVIFTVDGFSFSGSGQNTGMAFVSLKNWSQRKGAENTAQAIALR
                     ATKELGTIRDATVFAMTPPAVDGLGQSNGFTFELLANGGTDRETLLQMRNQLIEKANQ
                     SPELHSVRANDLPQMPQLQVDIDSNKAVSLGLSLNDVTDTLSSAWGGTYVNDFIDCGR
                     VKKVYIQGDSEFRSAPSDLGKWFVRGSDNAMTPFSAFATTRWLYGPERLVRYNGSAAY
                     EIQGENATGFSSGDAMTKMEELANSLPAGTTWAWSGLSLQEKLASGQALSLYAVSILV
                     VFLCLAALYESWSVPFSVILVIPLGLLGAALAAWMRDLNNDVYFQVALLTTIGLSSKN
                     AILIVEFAEAAVAEGYSLSRAALRAAQTRLRPIIMTSLAFIAGVMPLAIATGAGANSR
                     IAIGTGIIGGTLTATLLAIFFVPLFFVLVKRLFAGKPRRQE"
BASE COUNT         2946 a         3445 c         3352 g         3153 t
ORIGIN      
        1 ggatcgtacc tgtgttcctg ttctcggttc cttaacccct tctgccgccg atccagagtt
       61 tatcggtcag atgctcctga ccatggtcga tgaagatggc gctccctttg acgttgagcc
      121 gcggaacgtt cttaaccgcc tgtggcagca gctgcgccag cgcggattgt tcccggtcgt
      181 agcggtagag ctggagttct atttactgga tcgccagcgc gacgctgaag ggtatctgca
      241 accgccctgc gcgcccggca ccgatgaccg caatacacaa agccaggttt actccgttga
      301 taacctcaac cacttcgccg acgtgctcaa tgatattgat gagctggcgc agttacagct
      361 gattccggca gatggcgcgg tcgctgaggc ctcgccgggt cagtttgaaa tcaatcttta
      421 ccataccgat aacgtgctgg aagcttgcga tgatgcgctg gcactaaaac gcctggtgcg
      481 tctgatggca gaaaagcata agatgcacgc cacttttatg gcgaagccgt atgaagagca
      541 cgcgggcagc gggatgcata tccatatcag tatgcaaaat aaccgtggcg agaacgtgct
      601 ttctgacgcg gaaggcgaag attcgccgct gctgaaaaag atgcttgccg ggatgattga
      661 cctgatgccg tcatcaatgg cgttgctggc accaaacgtg aactcgtatc gccgcttcca
      721 gccaggtatg tatgtaccga cgcaggcgtc gtggggccat aacaaccgca ccgtcgccct
      781 gcgtattccc tgcggcgatc gccataatca ccgcgtggaa tatcgcgtgg cgggtgccga
      841 tgccaacccg tatctggtga tggcggcgat ttttgccggt attttgcatg gccttgataa
      901 cgaactgcca ttacaggaag aagtcgaagg taacgggctg gaacaggaag gcttaccctt
      961 cccgattcgc cagagcgatg ccctgggtga gtttatcgag aatgatcacc tgcgccgcta
     1021 tttaggcgaa cgcttctgcc atgtctatca cgcctgtaaa aatgatgagc tgttgcagtt
     1081 tgagcgcctc atcacagaaa cagaaattga gtggatgttg aaaaacgcgt aatacccctt
     1141 gcggggttgt ttcatcggcc tgagcggagc caggcctgac atttgctggt aatccagaca
     1201 tttcccggat gtcgcgttat ggagcgcagg cggcaacggg cttccgttaa cgattaagac
     1261 tgtgacgagg aaatgagatg atgccgatga ttaaatcccc gcatggcgag ggaggctgcg
     1321 tatgcgcccc tcctgcgact gactggactc cgcccccttt gcttcccttg ctaaacaggt
     1381 ttgatttcag gtcgacacga ccgcaaacct tattacgcag gggaggcagc aactatggct
     1441 attaattcac cactgactat tgctgcgcaa tctggcaaaa cccgtctgcg aaaatcactg
     1501 aaattgtggc aggtggtgat gatgggtctg gcctatctca cgccgatgac cgtatttgat
     1561 acttttggta ttgtctccgg cattagcgac ggtcacgttc cggcgtccta tttgctggcg
     1621 ctggcgggcg tgctgtttac cgctatcagc tacggcaaac tggttcgcca gtttccggaa
     1681 gccggttcgg cctataccta cgcgcaaaag tcgattaacc cgcacgtcgg atttatggtc
     1741 ggctggtcat cgctgctgga ttatctcttt ttgccgatga tcaacgtctt gttggcgaaa
     1801 atctatctct ccgccctctt cccggaagtg ccgccgtggg tgtgggtagt aaccttcgtc
     1861 gccattttaa ccgccgcgaa tctgaagagc gtcaacctgg ttgctaactt caataccctg
     1921 tttgtgttgg tgcaaatctc catcatggtg gtgtttatct tcctggtggt tcagggactg
     1981 cataaaggag aaggcgttgg caccgtctgg tcacttcagc cgtttatcag cgagaacgcg
     2041 cacctgatcc cgattattac cggggcgacg attgtctgtt tctccttcct cggtttcgat
     2101 gcggtgacca cgctttcgga agagacgccg gacgccgcac gcgtgatccc gaaagccatc
     2161 ttcctgacgg cggtctacgg tggcgttatc tttatcgctg cgtcgttctt tatgcagctg
     2221 ttctttcctg atatcagccg ctttaaagac ccggacgccg cactgcctga aattgcgctg
     2281 tacgtcggcg gcaagctctt ccagtcgatt ttcctctgta ccacgtttgt gaacacgtta
     2341 gcgtctggcc tggcgtcgca cgccagcgtg tcgcgtctgc tgtatgtgat ggggcgtgac
     2401 aatgtgtttc cggagcgcgt gtttggctat gtgcacccga aatggcgcac gccagcgttg
     2461 aacgtcatta tggtcgggat tgttgcgctg tcggcgctgt tctttgattt agtcaccgcg
     2521 acagcattga ttaacttcgg tgctctggtg gcgtttacct tcgtgaatct gtcggtgttt
     2581 aatcatttct ggcggcgcaa aggaatgaat aaaagctgga aggatcactt ccactatttg
     2641 ctgatgccgc tggttggcgc gctgacggtg ggtgtgctgt gggttaacct cgagtcaacg
     2701 tcactgacac tcggtctggt atgggcttca ttaggcggcg catatttgtg gtatctgatc
     2761 cgccgctatc gcaaagtgcc gttgtatgat ggtgacagaa cgccggtgag cgaaacgtaa
     2821 ttgtcggatg cgcggcgcat tatcctgcct gagggtaatg cgccgcgcca ttaactataa
     2881 aaagatgtat gacccggatc acgagtgcta tcatccgccc ttatgcttag cgcattaacc
     2941 ccaggggcaa ataatgaacc acgatatccc gctaaaatat ttcgatattg ccgatgagta
     3001 cgcgaccgaa tgcgcagagc ctgtcgccga cgctgaacgc acaccgctgg ccctctactt
     3061 ccagttactg ctcacccgtt tgatgaacaa tgaagaaatc agcgaagaag cccaacatga
     3121 aatggctgct gaagcgggga ttaatcccgt acgcattgat gagatcgcag agtttttgaa
     3181 tcaatggggt aatgagtaat tatttcattg cagacgatcg gtcagtatcg ttgtccggat
     3241 aaagcgttca cgccgcatcc ggctaacaat gacacgcact gcagtgtgcc tcaatgggtt
     3301 tctcacgatc gtaccgctct gctcctcgct aagttgttct gtgagttata gttatagaga
     3361 aatcacgggt acgaatgttg aacagatggc gacgccacct gttcaacgac caaccacacg
     3421 gccctcatta cgacccctga acacaaacgg taatggcaaa tgtcattaaa tattagtaag
     3481 atagcctctt tacataaaat cccttaatat tatgcgccag gtattatcgt ctcttttggt
     3541 gattgctgga cttgtgagtg gtcaggcaat cgccgcgcct gaatcccccc cgcatgctga
     3601 tatccgcgac agcggttttg tctattgcgt cagcgggcaa gtcaacacct ttaacccatc
     3661 caaagcgagc agtgggttaa ttgtcgatac ccttgccgcc cagttttatg atcgactgct
     3721 ggatgtcgat ccctatacct atcgcctgat gccggaactt gccgaaagct gggaagtact
     3781 cgacaacggc gcgacctatc gcttccacct gcgtcgcgat gttccgtttc aaaaaaccga
     3841 ctggtttact cccactcgta aaatgaatgc cgacgatgtg gtgtttacct tccagcgaat
     3901 ttttgaccgc aacaacccgt ggcataacgt taatggcagc aacttcccct atttcgacag
     3961 cctgcaattt gccgataacg tgaaaagcgt ccgtaaactg gataatcata ccgttgagtt
     4021 ccgtctggct cagccggatg cttctttttt gtggcacctc gcgacccatt atgcttcggt
     4081 catgtcggca gaatatgcag ggaagttaga gaaagaagat cgccaggagc aactcgaccg
     4141 tcaaccggtc ggcaccggtc cgtatcagtt gtcggaatac cgcgccgggc aatatattcg
     4201 cttacaacgt catgatgact tctggcgtgg taagccgtta atgccgcagg tggtggtgga
     4261 tttaggctcc ggcggcaccg gacgtctgtc gaaactcctg accggggaat gcgacgttct
     4321 ggcctggcct gccgccagtc agctatccat tttgcgtgac gacccgcgct tgcgtttaac
     4381 gctgcgcccg gggatgaacg tcgcctatct ggcgtttaac accgctaaac cgccgctaaa
     4441 taatccggcg gtccgccatg cactggcact ggcgattaat aaccagcgcc tgatgcaatc
     4501 catctattat ggtacggctg aaacggcggc ctctatttta ccgcgcgcct cgtgggccta
     4561 tgacaacgag gctaaaatta ctgaatacaa tccggcaaaa tcgcgcgaac agttgaaggc
     4621 gttggggctg gaaaacttaa cgctgaaact gtgggtgccc acacgttcgc aggcgtggaa
     4681 ccccagtcca ctgaaaaccg ccgaactgat tcaggcggat atggcacagg ttggcgtaaa
     4741 agtggtgatt gtgccggtag aaggtcgttt tcaggaggcg cggttgatgg atatgagcca
     4801 tgatctgacg ttatccggtt gggcgacgga cagtaacgac ccggacagtt tcttccgtcc
     4861 gttactgagc tgcgcggcaa ttcattcaca gaccaacctc gcccactggt gcgatccgaa
     4921 attcgacagc gtattgcgta aggcgctctc ttcgcagcag ctggcggcgc gtattgaagc
     4981 ctatgacgaa gcgcaaagta ttctggcgca ggaattacct attctgccgc tggcgtcgtc
     5041 attgcgtttg caggcctatc ggtacgatat caaaggtctg gtacttagcc cgtttggtaa
     5101 cgcctccttt gctggggtgt atcgcgagaa acaggatgag gtgaaaaagc catgattatc
     5161 ttcaccttac gccgtatttt gttattgatt gtcaccttgt tcctgctgac cttcgttggc
     5221 ttcagcttga gctatttcac cccacatgcg ccgttacaag gcgcgtcact gtggaatgcc
     5281 tgggtgttct ggtttaacgg cctgatccac tgggattttg gtgtttccag cattaacggt
     5341 caaccgattg ccgaacagtt aaaagaggtc ttcccggcga cgatggagct gtgcatcctc
     5401 gcctttggtt ttgcgctgat tgttggcatc cccatgggaa tgattgccgg gattacgcgc
     5461 cataagtggc aggataactt gatcaacgcc atcgccttac tgggcttttc aatcccggtg
     5521 ttctggctgg cgcttctgct gacgctgttt tgttcactca cgctaggttg gttgcccgtt
     5581 tctgggcgtt tcgatttgct ctacgaagtg aaaccgatta ccggttttgc gttgattgat
     5641 gcctggcttt cggactcacc gtggcgggat gaaatggtca tgagcgcaat tcgccatatg
     5701 atattacccg tgatcaccct gtcggttgcg ccaacaactg aagtgatccg actaatgcgt
     5761 atcagcacta tcgaagtgta tgaccagaac tatgttaaag cagcggcgac ccgcggtttg
     5821 tcgcgcttta ctattttgcg tcgccacgtt ctgcataacg cgttacctcc ggttattcct
     5881 cgcctgggct tacagttttc taccatgctg acgctggcga tgatcaccga aatggtcttt
     5941 agctggccgg gtttagggcg ctggttaatt aacgctatcc gccagcagga ttacgcagcc
     6001 atttccgccg gagtgatggt gtgtggctca ctggtcatta ttgttaacgt gatttctgat
     6061 attttgggtg ccatggctaa ccctctgaaa cataaggaat ggtatgcctt acgatagcgt
     6121 atacagcgaa aagcgcccgc cgggcacgct gcgtaccgcc tggcgcaaat tttatagtga
     6181 cgcctctgcg atggtcggcc tgtacggctg cgcggggctg gctgtactgt gtatttttgg
     6241 cggctggttt gcgccttacg gcatcgatca gcaatttctc ggttatcaat tattgccgcc
     6301 gtcatggtcg cgctatggcg aagtttcttt cttccttggg actgacgacc tcggacgcga
     6361 tgttttaagc cgtttactga gcggagctgc gccaaccgta ggcggcgcat ttgtggtgac
     6421 gcttgccgcg acgatctgtg gcctggtact gggcaccttc gccggggcga cccacggcct
     6481 ccgatcagcg gtgcttaacc atattctgga taccttactg gcgatccctt ccctactgct
     6541 ggcaattatc gttgtagcgt ttgccggacc gagtttgtcg cacgccatgt ttgccgtctg
     6601 gctggcgctg ctgcctcgta tggtacgttc gatttacagc atggtgcatg acgaactgga
     6661 aaaagagtac gttatcgccg cccgtctgga tggcgcatca acgctgaata ttctctggtt
     6721 tgctgtgatg ccaaacatca ccgccgggct ggtcaccgag atcacccgcg cactgtcgat
     6781 ggcaattctc gatatcgccg cgctgggctt tctcgatctc ggcgcacaac tcccctcgcc
     6841 tgaatgggga gcgatgctcg gtgatgcgct ggaactgatt tatgtcgccc cgtggactgt
     6901 catgctgcca ggtgcggcaa ttatgattag cgtgctgttg gttaacctcc ttggcgacgg
     6961 tgtccgtcgt gcaattattg cgggggtgga ataatgccat tactcgatat tcgtaacctg
     7021 accattgaat ttaaaaccgg tgatgagtgg gttaaagccg tcgaccgcgt aagtatgacc
     7081 ttaaccgaag gtgaaatccg cggtcttgtt ggcgaatcag gttccggcaa aagtttgatt
     7141 gcgaaagcaa tttgtggggt gaataaagat aactggcgtg ttactgctga ccgtatgcgt
     7201 tttgatgata tcgatttgct gcgtctctcc gcacgcgaac ggcgcaagct ggttggtcac
     7261 aacgtgtcga tgattttcca ggaaccgcag tcgtgtcttg acccttcaga acgtgtgggt
     7321 cgccagttga tgcaaaacat cccagcctgg acctataaag gccgttggtg gcagcgtttt
     7381 ggctggcgca aacgccgtgc cattgaactg ctgcaccgcg tggggattaa agatcacaaa
     7441 gatgcgatgc gcagttttcc ctatgagttg accgaaggtg aatgtcagaa agtgatgata
     7501 gccattgcac tggcgaatca accgcgtctg ctgattgctg acgaaccgac aaactcaatg
     7561 gagccaacaa cccaggcgca aatctttcgc ctgctgacgc gtctcaacca aaacagtaat
     7621 accactattt tgcttatcag ccatgactta caaatgctta gccaatgggc ggataaaatt
     7681 aacgtgcttt actgcggtca aacggtggaa accgcgccaa gtaaggagtt ggtgacaatg
     7741 ccacatcatc cttataccca ggcgctgatc cgcgcgatac cagacttcgg cagcgcgatg
     7801 ccgcataaaa gtcgcctcaa tacgctgccc ggcgctatcc cgctgctgga acagttaccg
     7861 attgggtgtc gtctggggcc gcgttgcccg tatgcacaac gagaatgcat tgtgacgcca
     7921 cgtttgacgg gggcgaaaaa tcatctctat gcctgtcatt tcccgctgaa catggagaaa
     7981 gaatgagatg atcgaaacgc tgcttgaagt gcgtaattta agtaaaacgt tccgctaccg
     8041 gaccggttgg tttcgtcgtc agaccgtaga agcggtaaaa cccttgagct ttacgctacg
     8101 tgaaggccag acactggcga ttattggcga gaatggttcg ggtaaatcca cgctggcaaa
     8161 aatgctggcg ggaatgatag aacctaccag cggcgagtta ttgattgacg atcatccgct
     8221 gcattttggc gattattcct tccgcagtca gcgtattcgc atgatttttc aggatccttc
     8281 gacctcgttg aatccccgtc aacgtatctc gcaaattctg gattttccac tgcgtctgaa
     8341 caccgatctg gaaccggaac agcgtcgcaa acagattatt gaaacgatgc gtatggttgg
     8401 gctgctaccg gatcacgtca gttactatcc gcatatgttg gcacccgggc aaaaacagcg
     8461 tctgggtctg gcgcgcgcgc tgatattgcg cccaaaagtc attattgccg atgaagccct
     8521 cgcctcactg gatatgtcga tgcgttcgca gttgattaac ctgatgctgg agttacagga
     8581 aaaacagggc atttcgtata tttatgttac ccagcatatc ggaatgatga agcacattag
     8641 cgaccaggtg ctggtgatgc atcagggcga ggttgtcgag cgaggcagca ccgcagatgt
     8701 gctggcatcg ccgctgcatg aactcaccaa acggctgatc gccggtcatt ttggcgaggc
     8761 attgacggcg gatgcgtggc gtaaggaccg ttagtgtttt gcacctcatc ctgacagggc
     8821 ccccctcagg atgaggtgtt cattactttt gattgtcgat tctggtgaga gcaaccagca
     8881 atgccagcgt ataacacacc gtaattgcca gactcatttt taacagtgtt tctccgccaa
     8941 ttcccgacaa tggcgcggct ataccgccga agacaaacat gctcatcccc atcaacgcag
     9001 acgccgttcc agattcctgc gtccctactg cactcatagc ctctgccccg ccaacagtgc
     9061 cgatgccgct acaaaatgcg atggtgaaaa ataatgccac cagtgccgga atgggcaatt
     9121 gtgtccatgc gcataagacc gtgagcaatg cacacaaaat tgccgctatc aggccacctc
     9181 gcaacaatgt catcgcgtta atccgtcgcg ccaggcgcga gaagatccat gaagcaatga
     9241 taagtccgat gccgttaagg ccaaacacca ggctaaattg cattggacta aagccaaatt
     9301 ccttctgcaa cacgaacgac gaagagccga tatatgcaaa caaaccggcc agcataaaac
     9361 tttgaatcag gcaaaagcgc ataaaacggc ggttctgcac cacgcttcgt ccggttaata
     9421 gcaatgatga gcccctttta ttttctggca aggtctcatt aataaataac aggcagccca
     9481 gcaacagtac ggtgctaatt tcagccatta cccagaataa agtgcgccag tcaaaagtgc
     9541 taacaatgta cccgcccagc actggcgaca acaccggtgc caggccattc accgtcatta
     9601 acagcgcaaa aaactgggtc aacgttactc cctgatattt gtcacgagca atagaacgag
     9661 agagcaccga accacccgcc cccgcgatcc cttgaataaa acgccagacc accagccagt
     9721 aaatattgtt cgttgtcgcg cacaaaatgg aagaaacaat aaacaatagc aacgacaaga
     9781 ttaacggacg tttacgccct attttgtcgc tcagagggcc aaataacagt tgtccgacgc
     9841 caagaccaat cagtgatgcg gtcagggtta attgcgttat ggtcgtggtt gccgccagtt
     9901 gctcagacat ctccggcaaa gccggcaaat aaagatcggt acacatcggg ccgataccgg
     9961 ctaaaagacc aagaatcaat gcccatgaaa gagagactct agccatatta ttccttactt
    10021 gagtgatgac atgccgccgc ccagcgactg ccagagcgtg attcggtttg taaaatcagt
    10081 cagttgcagt gccagcagct cctgctgtgc gctccacaag ctgcgctgcg ccgtcaacac
    10141 ggtcaggtaa tcaccaacac ccgcctgata gcgacgcaag ccaacatcga ccgtttgttg
    10201 ttcagcttta acatactggc gctgtgcatc cagttgctct tcaagcgtgg tccgccgcgc
    10261 cagtgcgtta gcgacatctt taaatgcgct ctgaacggtt ttttcatagg tggcaatgag
    10321 tccacgcttt tgtgcttctg cgtaacgaag ctgcgccaga ttacttccgc cagtaaacag
    10381 cggtaatgtg accgatggcg caaatgacca gatctgcatt ccgtggctga acaacgacga
    10441 caacgcgtca ctgccaacac cggcactggc agtcagcgaa atcgtcggga aaaagttagc
    10501 ccgtgccgcg ccaatatcgg cattggcgct cttcaaatta tgttccgctt cctggatatc
    10561 cgggcgacgc agcagaacat cagatgatac tcccgcagga accagactta tcatttgttc
    10621 cggcaggctt tccagtgttc ccggtagcag attttccgca agcgttgtac ctgccagtaa
    10681 gttcagcgca tttttatcct gcatcacttg tgtctgatag ctggcaacgc tggcgcgcgc
    10741 ttgctgataa acactcatcg cctcactgac atctgtcgct gccgccgtgc caacctgttg
    10801 ctggcgctgg ataattttca atgagttttc tgcactggcc atcgtttctt ttgccagggc
    10861 caggttgctg ttatcagccg ccagtgtcag ccaggcggtg ctgatttcag caatcagcgt
    10921 taaacgagtg ttctgggctg taaattcact ggcaagccag gtttcccgcg ccgcccgaga
    10981 taaactctga ttacgaccaa acagatctaa cgtataactg gacaccgtgc cgtcagcctc
    11041 ggcagttgtt cccgtaccat tcgccagcga acgactgcga gtcgaactta aggccgcatt
    11101 caccgtcgga aataacgacg cattagtttg cccatagagc gcccgtgctg agtcaatatc
    11161 agcaatcgct ttttgcacat cgcggttact gttcagtgca atggttacta cctgctgcaa
    11221 tcggggatcg tgaatcacct gctgcaatcg gggatcgtga atcacctgct gccagtcatg
    11281 gctgattgcc ttcccctggc cctgtgctcc cggcaaggtc gccgggattg gggattcagg
    11341 cgtactgtag tgaggatcga gcgagacaca tcccgcactc aacaacacca gaaaaataag
    11401 agaacgacgc aacataactt actcctgacg gcgcggttta ccggcaaaca aacgtttcac
    11461 cagtacaaaa aacagaggaa cgaagaaaat ggccagtaat gtagcggtca gcgtgccgcc
    11521 aataatgccc gtaccaatgg cgatgcggct gttcgcccct gcgccagttg ctatcgccag
    11581 cggcattacc cccgcaataa acgccagcga ggtcatgatg attgggcgta aacgagtctg
    11641 cgccgcgcgt aatgccgcac gactcaagga atagccctct gcaaccgccg cttcagcaaa
    11701 ttccacaatc agaatggcgt ttttcgatga caagccgata gtagttaata gcgctacctg
    11761 gaagtaaacg tcgttgttta aatcacgcat ccaggccgcc agcgccgcgc caagaagccc
    11821 cagagggatc accagaatga ccgagaacgg gactgaccag ctttcgtaca gtgctgcaag
    11881 gcagaggaaa accaccagaa tagaaactgc atacagactt aatgcctgac cgctggccag
    11941 tttctcctgc aatgacaaac cactccaggc ccaggttgtt cccgcaggaa gactgtttgc
    12001 cagttcctcc atttttgtca ttgcatcgcc ggaactaaag ccagtcgcgt tttcgccctg
    12061 aatttcatag gctgccgagc cgttgtagcg caccagtctt tccggtccat acagccagcg
    12121 ggtggtcgca aaagcagaga atggtgtcat agcgttatcg ctaccgcgca caaaccattt
    12181 acctaagtct gacggcgcgg aacgaaattc gctgtcgccc tgaatgtaga cttttttcac
    12241 acgaccacag tcaataaagt cattcacata agtaccgccc cacgcactgg acagggtgtc
    12301 ggtgacatca ttcaaactca accctaatga caccgcttta ttgctatcaa tatctacctg
    12361 caattgcggc atttgtggca aatcattggc gcgtacagaa tgtaactccg gactttgatt
    12421 cgctttttct atcaactgat tacgcatttg cagcagtgtt tcacgatcgg ttccaccgtt
    12481 agctaacaat tcaaacgtaa aaccattgct ttgccccagc ccatcaacgg ctggcggcgt
    12541 catcgcaaat accgtcgcat cacgaattgt gcccagctct ttggttgccc gtagggcgat
    12601 agcctgggcg gtgttttctg cccctttacg ttgagaccag tttttcaacg aaacaaacgc
    12661 catcccggtg ttctgtccgc tgccgctaaa actgaaacca tcaacggtaa agatgacatc
    12721 ggtatttgct ttctcgttaa tcaggaacca gtcaacaatc tggcgattca cttctgctgt
    12781 acgggcagcg gtcgccccgg ccggcagcgt gtactgcacc atgatttcgc cctggtcttc
    12841 agtgggtaag aaactgccgg gcagtttcca catcatcagg gccattccac caccca
//
LOCUS       DAMZFT010000132        12590 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.132, whole genome shotgun sequence.
ACCESSION   DAMZFT010000132 DAMZFT010000000
VERSION     DAMZFT010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12590)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12590)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12590
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.132"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..1308
                     /locus_tag="PN977_003999"
     CDS             <1..1308
                     /locus_tag="PN977_003999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000691807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DotA/TraY family protein"
                     /protein_id="HDI6043979.1"
                     /translation="YLTRRDTGNGNLPDIESRIQKAANRYEETVQKALPTDNAETERR
                     EQLKSYLTTYGWVALGAWYQTFATANQRLAELADRAPTVSGVSSLGEAGDTEFLKEVK
                     GAYQAQLQNSAYTPALGTTTSASEYHASNTQNPQSALLEFIGPGLTRFSNKVATIHSG
                     TGTTSQQANPLLKMKDIGDYTMVTTETIWAGYTTVRVLSTAGSNSVFGRLVNVVSGLS
                     DAAKAFLEASAPPLYFLLFLLFCAGFSLSIYLPFIPFIFWMTGIGNWIVSVLIGCAAG
                     PLWAATHLGTSQDRGSRAAYGYIYLIDSMIRPLLMVFGFFFASVAIVAGGTILNALFA
                     AALMNVQINSVTGVFSMAGFLMIYARACTTLVAAIFALQAYLPDHVINFLGGRDGVNM
                     LGNMVSSVKDIIAGGNTNMRRTPGVREEKLKNINRDDKDGIKQ"
     gene            1397..2047
                     /gene="excA"
                     /locus_tag="PN977_004000"
     CDS             1397..2047
                     /gene="excA"
                     /locus_tag="PN977_004000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid IncI1-type surface exclusion protein
                     ExcA"
                     /protein_id="HDI6043980.1"
                     /translation="MNKTKSRYPSWWAYYHLARNAYFLLGIPCLLLYSAAYTSDLLDD
                     NLRHSISLFILAYGCYFLLFIPPLWLYLRTRAKKKKVQQVVQQIKESSPFAPTSDYEQ
                     LSFSKSCYFGIDIKNGTMLYVRIYPNNVMDVIGLDIHNFTRTVAEDGKLEIHTTYVSL
                     PMIPLEISGISSRTLANTMHTMAARGYEYKERFPQMIRYRVKEWEKVAGVPVAEVF"
     gene            2203..2484
                     /locus_tag="PN977_004001"
     CDS             2203..2484
                     /locus_tag="PN977_004001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HDI6043981.1"
                     /translation="MPSATQTARLEARISTELHAMLKRAAEIQGRTMTDFIISAVQDA
                     AQRAIEQTDIIRLSRTDQEIFAQALLSPTEPNDALKRAFKRRNNILYTK"
     gene            2490..2978
                     /locus_tag="PN977_004002"
     CDS             2490..2978
                     /locus_tag="PN977_004002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001443703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDI6043982.1"
                     /translation="MFQLKPFNSTYDRTSFLCGSDALDRYIKEHVTQDIRRRIAACFV
                     AIDNDHRIIGYYTLSAASILLTDLPEHLRKKLPRYPTIPAIRMGRLAVSLSSKGTGLG
                     GTLLADALYRAYTSEIAAYALIVDAKDDTAVSFYKHYGFMTLPESGRTLFMPLASLSA
                     IE"
     gene            complement(3065..3217)
                     /locus_tag="PN977_004003"
     CDS             complement(3065..3217)
                     /locus_tag="PN977_004003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001618166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HDI6043983.1"
                     /translation="MPQRTFLIMLIVVCVTILCFVWMVRDSLCGFRVEQGNTVLVATL
                     AYEVKR"
     gene            3546..3788
                     /locus_tag="PN977_004004"
     CDS             3546..3788
                     /locus_tag="PN977_004004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6043984.1"
                     /translation="MKDEIQSMCLTPDIVKQFNTLLNTHCQSSEEALGMLSALCFELM
                     MKCGVQSINSLMVNGMGISMQLQGIDIAERPGKNIH"
     gene            3884..4075
                     /locus_tag="PN977_004005"
     CDS             3884..4075
                     /locus_tag="PN977_004005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001322947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6043985.1"
                     /translation="MKKTKTHTGLLIIKDKTRRVSLYETPTAWCIRGQECYSKSTGRR
                     CGSHDSLSRLRLDSIKPVE"
     gene            4087..4407
                     /locus_tag="PN977_004006"
                     /pseudo
     CDS             4087..4407
                     /locus_tag="PN977_004006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001456887.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(4685..5308)
                     /locus_tag="PN977_004007"
     CDS             complement(4685..5308)
                     /locus_tag="PN977_004007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001579788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HDI6043986.1"
                     /translation="MQEHIDYQIEKYQFRARNESPRLMRQWEDVMRECRDTGAGARER
                     LRIALLNVDYVTSFELPFRLLLTRTPQMIDSVRREWQLSQKKVVFNERQRGCVYSLQN
                     DLSGVPDSFSYSLATRIRRVDSYGMTTRCFTDIAGQVKAPTARLKLALESGLIVTALD
                     GLFWLGIQRIAADVQRLRQSGMTIITRDVEVSDSLTGTTRVVSGYSL"
     gene            5468..5935
                     /locus_tag="PN977_004008"
     CDS             5468..5935
                     /locus_tag="PN977_004008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000598960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HDI6043987.1"
                     /translation="MIKYIFSLTIIFSASLCAAQIQGHVIRVLDGDTLEILQDKKPVR
                     VRLANIDAPEKKQPFGRWSATQLKDLVAGKPVTVQYAQTDRYGRILGRVYTADGTEAN
                     RWQVRNGAAWVYPDYNNDYTLPPLQRDARALKRGLWADKNPVPPWTWRKQNKK"
     gene            6086..6262
                     /locus_tag="PN977_004009"
     CDS             6086..6262
                     /locus_tag="PN977_004009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001054900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6043988.1"
                     /translation="MNLVDAFVTKVISGPYEEYGKWWVDVEYISWGVPGKSRLMFGSR
                     EQAQEVQEGFRFLT"
     gene            6719..8035
                     /gene="trbA"
                     /locus_tag="PN977_004010"
     CDS             6719..8035
                     /gene="trbA"
                     /locus_tag="PN977_004010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001513486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type conjugal transfer protein TrbA"
                     /protein_id="HDI6043989.1"
                     /translation="MSYQQTSAAEDPMAIWYIVGAICLLFAIIIWRFLPEIVFASCLI
                     LHTLWGMIDWGPFHNFAAPRYNLLAITANNAATITFSQWLDVMSRTVGILWLILLPMT
                     FGFLWMWFHHPAQPRFTRRPLNIHTLPHIFSALSPAIAPVLADGDNNRLFHGQKRPER
                     RVALTPEAFVEQNNLIRNMQLDVASTRQCFMAQLGQPLTSWKDMAPHEKALFAIFGLQ
                     FFLGDRKAAVALMNNLNLSCRLKSKRDQGRFSTPVYSLARNAFIRVIKTEGAQKWLRQ
                     HRYVRSGLVWLYAHDLRLTPPNWLWLKGVDRTLFYALHRANTTKGFIEGAGVVAVARA
                     ENEASRLGLPCPEPCVEEAIEGLRRDMLGLGLIWDEPQPDRDRKRQIRTRWSLTDDVI
                     PRRHDNDEDTDTGETTETTETTETTETTETTETTEKQHPADKEKAQ"
     gene            8032..9150
                     /locus_tag="PN977_004011"
     CDS             8032..9150
                     /locus_tag="PN977_004011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004031782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbC family protein"
                     /protein_id="HDI6043990.1"
                     /translation="MTIRYTATLNNKHLSLTALYPDGGQEVIAILPVTELGALINRNN
                     RLMHLSFSTPFREITETLLVDTVDTERPGHHIGRMLSAIDSCLSAAYAELPARKTSFM
                     RGLIYGAGLVLALLTAVTAGYILLKPDSDSPLRVPVTESVTETAPLHIDKEIVRSVAP
                     SETKNEIVHPASQEMQETSATPAITADKNTLVAEARRNLATVLKRNADRGMFTINLST
                     GHERTLYAFLDPACINCRLLEPALKQLAREFSVVIYPVSVIGGEASTNRTAPLLCEPD
                     SQKRATGWHQLYSADGGMAPTGDAPAAPDEKCLTAARAAIDVNNQAFRQFGFGGTPWV
                     LSDTGWHLPSGILKDATTVSLFLKATDTEDNHNEPASR"
     gene            9131..11431
                     /locus_tag="PN977_004012"
     CDS             9131..11431
                     /locus_tag="PN977_004012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000077650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="HDI6043991.1"
                     /translation="MSQHHVNPDLIHRTAWGNPLWNALHNLNIIGLCLAGSIITALIW
                     PLALPVCLLFTLVTSVIFTLQRWRCPLRMPMTLSLDDPSQDRKVRRSLFSFWPTLFQY
                     EADETSPARGIFYVGYRRINDIGRELWLSMDDLTRHIIFFSTTGGGKTETTFAWLLNP
                     LCWGRGFTFVDGKAQNDTTRTIWYLSRRFGREDDIEVINFMNGGKSRSEIIQSGEKSR
                     PQSNTWNPFAFSTEAFTAETMQSMLPQNVQGGEWQSRAIAMNKALVFGTKFWCVRERK
                     TMSLQMLREHMTLEGMAKLYCRGLDDQWPEEAIAPLRNYLQDVPGFDMSLVRTPSAWT
                     EEPRKQHAYLSGQFSETFTTFAETFGDVFAADAGDIDIRDSIHSDRILIVMIPALDTS
                     AHTTSALGRMFITQQSMILARDLGYRLEGTDAQTLEVKKYKGRFPYICILDEVGAYYT
                     DRIAVEATQVRSLEFSLIMTAQDQERIEGQTSATNTATLMQNAGTKFAGRIVSDDKTA
                     RTVKNAAGEEARARMGSLQRHDGVMGESWVDGNQITIQMESKIDVQDLIRLNAGEFFT
                     VFQGDVVPSASVYIPDSEKSCDSDPVVINRYIAIEAPRLERLRRLVPRTVQRRLPTPE
                     HVSSIIGVLTEKTSRKRRKKHTEPYRIIDTFQDQLKRTQTSLDLLPQYDTDIESRANE
                     LWKKAVHTINNTTREERRVCYITLNRPEEEHSGPEDIPSVKAILNTLLPLEILLPVAE
                     NNKSQTINNDHPQKSCGAQPTTDEKY"
     gene            complement(11652..>12590)
                     /locus_tag="PN977_004013"
     CDS             complement(11652..>12590)
                     /locus_tag="PN977_004013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000991381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase NikB"
                     /protein_id="HDI6043992.1"
                     /translation="GFRRDPELRRERREARAAAREDLRARYLAWKEHWRKPDLRYGER
                     LREIHAACRRRKAHIRVQFRDPQVRKLHYHIAEVQRMQALIRLKENVREERLALMAEG
                     KWYPLSYRQWVEQQAAQGDRAAVSQLRGWDYRDRRSRNKDKRRTTNADRCVVLCEPGG
                     TPLFNNVAKLEARLQKNGSVHFRDTRTGKNVCTDYGDRVVFYHHTDRNELAEKLDLIA
                     PVLFSRNGKLGFEPEGSYQQFNDVFAEMVAWHNAAGITGNGHFTITRPDVDLHRQRSE
                     QYYREYIRQQTRLSESHDDNYTLRQEKTWEPPSPGM"
BASE COUNT         3274 a         3417 c         2970 g         2929 t
ORIGIN      
        1 tacctgaccc gacgcgacac gggtaacggc aacctgccgg atattgaaag ccgtattcag
       61 aaggctgcca accgatatga agaaacggtg cagaaggcgt tgccaacaga taacgcagaa
      121 actgagcgca gggagcagct gaaatcctac ctgaccacct acggctgggt tgccctgggg
      181 gcctggtacc agacctttgc caccgccaac cagcgactgg cagaactggc cgaccgtgcc
      241 cccactgtga gtggcgtctc gtcactgggc gaagccgggg atacggagtt cctgaaggaa
      301 gtcaaggggg cttaccaggc acagctacag aactcagcgt atactcctgc actgggaaca
      361 accacctctg ccagtgaata ccacgcatct aatactcaaa atcctcaatc agccctgtta
      421 gagttcatag ggccagggtt aactcgattt tcaaataaag tagcaaccat acattcagga
      481 acaggaacaa cgagtcagca ggcaaatcct ctgctgaaga tgaaggacat tggtgattac
      541 accatggtca caaccgaaac catctgggcg ggttatacca ccgtcagggt gctttctact
      601 gccggcagca actccgtttt tggccgactg gtcaatgtcg tcagcggctt atctgatgca
      661 gcaaaggctt ttctggaagc ctccgcacca ccactctact tcctgctgtt tttgctgttc
      721 tgtgccggtt tttcgttatc tatttacctg ccgtttatcc cgtttatctt ctggatgacc
      781 ggcatcggca actggatagt cagcgtactg attggatgtg ctgctggccc cctgtgggcc
      841 gccacccatc tgggaacaag tcaggaccgg ggaagccggg ccgcttacgg ttacatctac
      901 ctgatagaca gcatgatcag accgctgctg atggtgttcg gattcttttt tgcatcggtc
      961 gccatcgtgg ccggcggcac aatcctgaat gcgctgtttg ctgctgcgct gatgaatgtc
     1021 cagataaatt ctgtgacggg cgtgttcagc atggcgggat ttttgatgat ttatgcccga
     1081 gcctgtacca cgctggttgc ggccattttt gcacttcagg cttatctgcc tgaccatgta
     1141 attaattttc tgggcgggcg tgacggtgtt aatatgctgg ggaatatggt ttcttcagtc
     1201 aaagatatta ttgccggagg taataccaat atgcgcagaa cacctggcgt tcgtgaagaa
     1261 aaactgaaaa atattaatag agatgataaa gacggaataa aacaataaaa tgaaaacata
     1321 gaaaaaacaa taaaacagca ttgccatatt cacctgaaga gtttaaaata tattcaggtg
     1381 aatatggagg tttattatga acaaaacaaa aagcagatat ccttcctggt gggcatatta
     1441 ccacttggcc cgcaacgctt atttcttatt aggaataccg tgtttacttt tatactcagc
     1501 cgcttataca tcagatttat tagatgataa tctaagacat tcaataagtc tttttattct
     1561 ggcatatgga tgttattttc ttcttttcat tcctccgctc tggttatatc tgagaaccag
     1621 agcgaagaaa aagaaagtcc agcaggttgt tcagcagata aaagaaagca gtccctttgc
     1681 gccgaccagc gattatgagc aactgtcatt ctcgaagagt tgttatttcg ggatcgacat
     1741 taaaaacggc accatgctgt atgtgcgtat ttatccaaac aatgtgatgg atgtgattgg
     1801 tctggatatt cacaacttta cccgcacggt tgctgaagac ggcaagctgg aaatccacac
     1861 cacctatgtg agtctgccga tgattcctct tgagatctcc ggcatctcat caaggacgct
     1921 ggccaacacc atgcacacca tggctgcacg tggatatgag tacaaggaac gtttcccaca
     1981 gatgatccgg tatcgggtta aagagtggga aaaagttgcc ggtgttcctg ttgcagaagt
     2041 attttgaggt agctatcaac tgttagacga taatccgtca gccggttttc tctgaaccgg
     2101 ctttttcttt tcaggcagaa tgcctgtctg ccgtccctgt atcagactgt aatttgcaca
     2161 atacggcaat ttgccgtaca gtgtattcag gaggtacaaa tcatgccatc tgcaacacaa
     2221 actgcccgac tggaagcaag aatcagcaca gaactgcatg ctatgctgaa acgcgctgca
     2281 gaaatccagg gacgcactat gaccgatttt atcatttctg ccgttcagga tgcggcacaa
     2341 cgcgctatag aacaaaccga tatcatccga ctgtcccgga cagatcagga aatttttgct
     2401 caggcattac tgtcaccaac agaacctaat gatgcgctca aacgggcctt taaacgccgt
     2461 aataatattc tatacacaaa ataaatataa tgtttcagct gaaaccattt aacagcacat
     2521 atgacagaac ctcattttta tgtggttctg atgctctgga ccgttatata aaagagcatg
     2581 tcacacagga tattcgacga cgcatcgcgg cctgctttgt tgctatagat aacgatcatc
     2641 gtatcatcgg atactatacg ttatcagcgg ccagtatatt attaactgac ttacctgagc
     2701 atttgagaaa aaagctacca cgatatccta ccatcccggc tattcgtatg ggacgcctgg
     2761 cagtaagtct gtcatcaaaa ggaacaggac ttggtgggac acttttagct gatgcgctat
     2821 atcgtgcata cacatctgaa attgcagcat atgcgctgat tgttgatgca aaagatgaca
     2881 cggcagtctc attttacaaa cattatggat ttatgacgct ccctgagtcc gggcgaactt
     2941 tattcatgcc actggcatca ttatctgcaa tagaataacc agaataaatc cgggtaaaaa
     3001 agggcgcatt gaggtcagcc ttcgcaacaa tccggcggca gatgtcctgc cgccgtgttg
     3061 cccgttaacg tttaacttcg taggctaacg ttgccacaag cactgtgttt ccctgctcga
     3121 cacggaatcc gcaaagcgaa tccctcacca tccagacaaa acacagtatc gtcacacaga
     3181 cgacgattaa cattattaaa aacgttcgtt gtggcatcgc agcccctcca ttgcctttcg
     3241 gccgtcatcg ggctactata atgttgtcta ggcattacag tgggccctgg gttgattgat
     3301 aaaaaaatcg cccggggctt tctgctacat gccgtccaca aaacgctttc ggcatacagc
     3361 ctcaagcgcc cgcagctatt ctaattgttt tcctgtccgc cacaatcctt ttcatgccgt
     3421 tatatttttt gtatccggaa caggaaaata cccgggaatt atcccatccg aacgcctcag
     3481 gttttccgtc tacgggcttc gtgttattac cccaaaatgc aaaatgcctg aaataaggag
     3541 tagtcatgaa agacgaaata caaagtatgt gcctcacccc ggatatcgtt aaacagttca
     3601 atactctttt gaatacacac tgccagtcct cagaagaggc gctcggcatg ctcagcgcac
     3661 tgtgttttga gctcatgatg aaatgtggtg ttcagtccat aaacagcctc atggtcaacg
     3721 gtatgggcat atccatgcaa ctccagggga ttgatattgc cgaaaggccc ggcaagaata
     3781 ttcactaacc tgaacggagc ctgaaaacgc tgatttttca ggccccagca ggctaaaact
     3841 gatgatgcaa tgacaacaaa atttctgcgt ttgtacccca ccgatgaaaa agacaaaaac
     3901 tcacaccggg cttctcatca ttaaagataa aacccgacgt gtcagcctct acgaaacgcc
     3961 aacggcatgg tgtatcaggg ggcaggagtg ctacagcaaa agcaccggac gccgctgtgg
     4021 cagccacgac agtctgagca gactgcgcct ggacagcatt aagcctgttg aatgagtggt
     4081 ggcaccatga aagataacaa cacaaccgcc accccagaag cctggaaaat gcgttttctg
     4141 gccaacggga catccggcac atggttttac actgacgaca ttgcagtcac tgacgcgtgg
     4201 cttcgcgcgc acccggatga tgtggaggtt atccctctct gccggataca gccccgccag
     4261 cattcccatg aacaacatat tgaagaactc cgcgcagccc tggcccggat gctccatatg
     4321 catcatatga tgctggaaac cacacattac cgggaaaata tcaccgatgc ccgttgtatc
     4381 cgtgaaatgc atgaagcgcc attaaaaagg agaatgttgg cgaattttct ctgcggggcg
     4441 tttgtgtttc tgacggaaat acgaaggagg aggctgagag ctcatgacgg atattactga
     4501 cagacataca cttcgccgta tgtcctggtc ggaactcatc acggcaggac aggaagcaga
     4561 atttcagcgt gattacgagc gtaccagaat agtgtggtca ttcgccttac atgttgcaac
     4621 aaccacgatt aacaaaaatc tggccatagc tcatattcga cgctgcgata ctttgttgca
     4681 taaatcaaag gctgtacccg gaaacaaccc gtgttgttcc ggtcagcgag tctgagacct
     4741 caacatcccg ggtgatgatt gtcattccgg actgacgcaa ccgctgcaca tctgcggcaa
     4801 tacgctgaat accaagccag aacagaccat ccagcgcggt gacaattaac ccgctttcca
     4861 gcgccagttt cagccgtgct gtcggggctt taacctgccc ggctatatca gtgaaacaac
     4921 gcgtcgtcat gccatagctg tccacccgcc gtattcgcgt cgcaagactg taactgaagc
     4981 tatccggcac gccggataag tcattctgca gactgtatac acatccccgc tgccgctcat
     5041 tgaatacgac ttttttctgg ctgagctgcc attcccgccg cacgctgtca atcatctgcg
     5101 gtgtccgggt taacagcaga cgaaacggca gttcaaaact ggtcacataa tcaacattaa
     5161 gtaaggcaat ccgcagccgc tccctggctc ctgctccggt atcacggcat tcacgcatga
     5221 catcttccca ctggcgcatc agtcgcggtg actcattcct ggccctgaac tgatatttct
     5281 caatctgata atcaatatgt tcctgcatac cgtcaccgga tatctgttgc cgtatcatca
     5341 cggatatcag cgtactggaa ttcatgccct gcgtgaagta tcacactgcc aaaaaaaaca
     5401 cacctgaacg ttattatttc gcaatattaa ttgcacctgt tttcataaat aaaaaggaga
     5461 cgatgaaatg ataaaatata tcttttcact caccattatt ttttccgctt ctctctgtgc
     5521 cgcacagatt caggggcacg tcatccgcgt tcttgatggt gatacgctgg aaatattaca
     5581 ggacaaaaag cctgtcaggg tccgtctggc taatattgat gcaccggaaa agaaacagcc
     5641 ctttggacgc tggtcagcca ctcagctgaa ggacctggtg gccggaaagc ccgtaaccgt
     5701 gcaatacgcg cagacagaca ggtatggccg tattctcggc cgggtctata ccgctgacgg
     5761 cacagaagca aatcgctggc aggtccgtaa cggcgctgcc tgggtgtacc cggactacaa
     5821 taatgactat actctcccgc cgctccagcg tgatgcccgc gccctgaagc ggggattatg
     5881 ggcagataaa aatccggtac caccatggac ctggagaaaa cagaataaga aataattatt
     5941 tgctgatatg aagaaaaaaa ccatttcaaa gaaaataaat gaaaaaaata cagtatggga
     6001 aaaaattgcc atttttaccg ccgcgttatt tttaatattt gctgtctgct ggtcgtaata
     6061 agcagaataa cagaaaggtg taaaaatgaa cctggttgat gcatttgtca ctaaagtgat
     6121 atccggaccg tatgaagaat acggaaaatg gtgggtggat gtagaatata tttcctgggg
     6181 agttccgggt aaaagccggc tcatgtttgg ctcacgggaa caggcacagg aagtgcagga
     6241 aggttttcga tttttaacct gagcatattt tgttaaaggg cagaaggagg tgaccctctg
     6301 cccttaagta cgtttccggg attatcagtc aaccagttcc cggagagcta tcactgcgtt
     6361 agccacagca acaaccgccc tgagaacgat gattaacagg ttaatcatcc gtttctccgt
     6421 caatggagga aatcactgct gcttactctt acattgcctg tcacaacagc tctctcattg
     6481 ttagctgaaa agagacttcc tccggtcaga gccaccaact ccaccccgga atcgcaaaaa
     6541 aaccggcctc accagtcggt ttttttgtac ctgtctccca ccaccttctg ccgttcgctc
     6601 ttctctgtaa aaatgtcctg ttgaattcat tcctgtccgg ttattaccgg cgctgattcg
     6661 cattcatgcc acccgactta atcccgaaat ttatccattc atctatggag gaatccccgt
     6721 gtcttaccaa cagacgtctg ccgccgaaga tccaatggct atatggtata tcgtcggtgc
     6781 catatgcctg ctctttgcga ttatcatctg gcgcttttta cctgaaatcg tcttcgcctc
     6841 ctgcctgatc ctgcacacac tgtggggcat gattgactgg gggccctttc acaactttgc
     6901 cgccccccgt tacaacctgc tggccatcac tgctaacaac gcggcaacca tcactttcag
     6961 ccagtggctg gatgttatgt cccggacagt ggggatcctc tggctgattc tgcttcccat
     7021 gacattcggc tttctgtgga tgtggtttca ccatccggcc cagccccgtt ttacccgcag
     7081 gccactgaat atccacacac tgccccatat tttttcagct ctgtcaccgg cgatcgcccc
     7141 cgttctggct gacggagaca acaacagact cttccacgga caaaaacgtc ccgagcgccg
     7201 tgtcgcacta acccccgaag cctttgtgga gcagaacaac cttatccgca acatgcagct
     7261 cgatgttgcg tccacacgcc agtgcttcat ggcgcaactg gggcaaccac tgaccagctg
     7321 gaaagacatg gccccacatg aaaaagcgct ctttgccatc tttggcctgc agttcttcct
     7381 cggtgaccgg aaagcagcgg tagccctgat gaacaacctc aatctctcct gcaggctcaa
     7441 aagtaaacgg gaccaggggc gtttcagcac gccggtgtat tcactggcca gaaacgcctt
     7501 tatccgggtc atcaaaacag aaggcgcaca aaagtggctc aggcagcacc gttacgttcg
     7561 cagcggtctg gtctggcttt acgcacatga tttgcgcctg acgcctccga actggctgtg
     7621 gctcaagggc gttgaccgga cgctcttcta tgcccttcac cgggcaaaca ccacaaaagg
     7681 cttcatcgaa ggggccggcg tggtggccgt tgcccgggca gaaaatgaag ccagccgtct
     7741 cggcctcccc tgcccggaac cctgtgtgga agaggccatt gaagggcttc gacgggacat
     7801 gctggggctg ggcctgatat gggacgaacc tcagccagat cgcgatcgta aacgtcagat
     7861 caggacccgc tggtcactca cggatgacgt gataccccgc cgccatgata acgacgaaga
     7921 cactgacact ggtgaaacca ccgaaaccac cgaaaccacc gaaaccaccg aaaccaccga
     7981 aaccaccgaa accaccgaaa aacagcatcc cgccgacaag gaaaaagcac aatgaccatc
     8041 cgctacactg caacactgaa caataaacac ctgtccctga ccgccctgta ccctgacggt
     8101 ggtcaggagg ttattgccat cctgccggtt acagaactgg gcgcactgat taaccgtaat
     8161 aacaggctga tgcacctgtc cttcagcacc ccttttcgtg aaataaccga aacactgctt
     8221 gttgataccg ttgatactga acgtcccgga catcacatcg gcagaatgct ctccgcgatt
     8281 gacagctgtc tgagcgcggc atatgcagaa cttcctgccc ggaaaaccag cttcatgaga
     8341 gggctgatat acggtgcagg attagtgctg gcacttctga cagcggtaac agccggatat
     8401 attttactga aaccggacag cgattcccct ctcagggtac ctgtcacaga atccgtaaca
     8461 gaaacagcac cattacacat cgacaaggaa attgtccggt cagtagcccc gtcagagacg
     8521 aaaaatgaaa tcgttcaccc ggcatcacag gaaatgcagg agacatccgc taccccggcg
     8581 ataacagcag acaaaaatac tctggtcgct gaagcccgcc gtaatctggc cactgtactt
     8641 aaacgcaacg cggaccgggg tatgttcacc attaatctct ccactggtca tgaacgcaca
     8701 ctctacgcct tccttgaccc cgcatgcatc aactgccgtc ttctggagcc cgcgcttaag
     8761 caactggccc gggaattcag cgtggtgatt tatccggtct ccgtaatcgg cggagaggcc
     8821 agcacaaacc ggactgcgcc actgctgtgt gaaccggact cacaaaaacg ggcgaccgga
     8881 tggcatcagc tgtattccgc agacggtggt atggcaccca ccggcgatgc accggctgca
     8941 ccggatgaaa agtgtctgac cgctgctcgt gctgccatcg atgtcaataa ccaggctttc
     9001 cgccagttcg gttttggcgg taccccatgg gtgttatccg ataccggctg gcacctgccg
     9061 tccggcatcc tgaaagacgc cactaccgtc agcctgttcc tgaaagccac agataccgag
     9121 gataatcaca atgagccagc atcacgttaa tcctgacctc atacaccgca cggcctgggg
     9181 aaacccgctg tggaatgcac tgcacaacct gaacatcatt ggtctgtgtc tggccggcag
     9241 tatcattacg gccctgatat ggccactggc ccttcctgtc tgtctgctgt tcaccctggt
     9301 cacctccgtg atattcacgc tgcaacgctg gcgatgcccc ctgcgtatgc ccatgacgct
     9361 cagccttgat gacccctccc aggacagaaa ggtcaggcga agcctcttca gcttctggcc
     9421 caccctgttt cagtatgaag cagatgaaac atcccccgca cgggggattt tttatgtcgg
     9481 ttaccgccgt atcaacgata tcggtcgtga gctctggctg agtatggatg acctcacccg
     9541 gcatatcata tttttttcca ccaccggtgg cggtaaaaca gaaaccacct ttgcctggct
     9601 gctcaaccct ctctgctggg gaagaggctt tacctttgtt gacggaaagg cacagaacga
     9661 taccacgagg acaatctggt atctctcccg acgttttggc cgggaggatg atatcgaggt
     9721 catcaacttc atgaacggcg gaaaatcccg cagtgaaatc attcagagcg gcgaaaaatc
     9781 ccgcccacag tcaaacacct ggaacccgtt cgcattcagc accgaagcct tcaccgccga
     9841 aaccatgcag tccatgctac cccagaacgt tcagggggga gaatggcagt cacgggcgat
     9901 agccatgaac aaagccctgg tctttggcac aaaattctgg tgcgtccggg aaagaaaaac
     9961 catgtccctg cagatgttac gggagcatat gacgctggaa ggtatggcaa aactctattg
    10021 ccgtgggctt gatgaccagt ggccggaaga agccatcgca ccactgagaa attacctgca
    10081 ggacgttccg ggattcgata tgtcactggt gagaacgccg tcagcctgga ctgaggagcc
    10141 ccgtaaacag catgcttatc tctccggtca gttctcggaa acattcacaa cctttgccga
    10201 aaccttcggg gacgtctttg cggcagatgc cggcgacatc gatattcgcg acagtatcca
    10261 cagcgaccga attctgattg tgatgatccc cgcactggac acatcagccc ataccacgtc
    10321 ggcactcggt cgtatgttca tcacccagca gagcatgatc ctcgcccgtg acctgggcta
    10381 ccgcctggaa ggcacagacg cacagaccct ggaagtcaaa aaatacaaag gccgcttccc
    10441 ctatatctgt atccttgacg aagtcggtgc ctactacaca gaccgcattg ctgtggaggc
    10501 cacccaggta cgttcactgg agttttccct gataatgacg gcgcaggatc aggagcgaat
    10561 tgaagggcag acctccgcca ccaacaccgc cacactgatg cagaacgccg gcaccaaatt
    10621 cgccggacgc attgtcagtg acgacaaaac ggcaagaacg gtgaaaaatg ccgccggtga
    10681 agaggcacgg gccagaatgg gcagcctgca gcgccacgat ggtgtgatgg gtgagtcctg
    10741 ggtggacggt aaccagatca ccatccagat ggaaagcaaa atcgacgtgc aggatttgat
    10801 cagactgaat gccggtgagt tctttaccgt tttccagggg gatgtggtgc cgtcagcctc
    10861 tgtgtatatt ccggacagcg agaaaagttg cgacagtgat ccggtggtca tcaaccgtta
    10921 tatcgccatt gaagcccccc gactggagcg tctccgccgt cttgtcccca ggacagtgca
    10981 gcgcaggctc cccacaccgg agcatgtcag cagcattatc ggtgtgctga cagaaaaaac
    11041 ctcccggaaa cgtcgtaaaa aacacacaga gccttaccga atcatcgata ccttccagga
    11101 ccagcttaaa agaacacaaa catcgctgga tctgcttccg caatatgata cagatatcga
    11161 atcccgggca aacgaactgt ggaaaaaggc tgtgcacact atcaataaca caaccaggga
    11221 agaacgccgg gtatgttata tcacactgaa tcgtccggaa gaggaacact ccggtcctga
    11281 agatatacct tcagtgaagg cgattctgaa taccctgctc cccctggaga tactgttacc
    11341 ggtcgctgaa aataataaat cgcagacaat aaataatgat catccccaga aatcatgtgg
    11401 agcacaacca acaactgacg aaaaatactg aaggaataca aatattttct ggtgaccata
    11461 tctgtcaatc agactgtaaa accctgttca ctaagtccgg ggttttacgg cgcactggat
    11521 aagggccgca gacacggccc ttaattcaga gttaaccggg tataaactca attcagtgac
    11581 tactcccgcc cttattgacg aaggttcctg ccgtttttgg gctgaatctt tgcaccatca
    11641 cttatccggg cttacattcc aggtgatggt ggctcccatg ttttctcctg ccgtaacgta
    11701 taattatcat catggctctc cgataaacgg gtctgctgtc tgatatattc ccggtaatac
    11761 tgctcactgc gttgacggtg caaatccacg tccggacgag taatggtgaa gtgtccgttt
    11821 ccggttattc ccgctgcatt gtgccaggcc accatttctg caaacacatc attaaactgc
    11881 tggtatgaac cttccggctc aaaaccaagc tttccgttgc ggctgaacag aactggcgca
    11941 attaaatcaa gcttctctgc cagttcattt cggtccgtgt gatggtagaa cacaacccga
    12001 tctccgtaat ccgtacagac attttttcct gtccgcgtat ccctgaaatg aacacttcca
    12061 tttttctgaa gacgagcttc aagttttgca acattattaa aaagtggcgt tcctccgggt
    12121 tcgcagagaa cgacacaacg atcagcatta gttgtgcgtc ttttatcctt attacgcgag
    12181 cgacgatcgc gataatccca gcctcggagc tgtgacactg cagctctgtc gccttgcgcc
    12241 gcctgttgtt ccacccactg ccggtaggac aacggatacc acttaccctc cgccatcagc
    12301 gccagtcgct cttccctgac attttccttc agtctgatca gcgcctgcat acgttgcact
    12361 tctgcaatat ggtaatgcag tttacgcact tgcgggtccc ggaactgcac gcggatatga
    12421 gccttgcgac gacggcaggc agcatgaatt tcccgcaacc gttcgccgta acgtaaatcg
    12481 ggtttacgcc agtgttcctt ccatgcaaga taacgcgcac gcaaatcttc ccgcgcagct
    12541 gcacgggctt cacggcgctc acgacgcagc tcaggatcgc gccggaagcc
//
LOCUS       DAMZFT010000133        12415 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.133, whole genome shotgun sequence.
ACCESSION   DAMZFT010000133 DAMZFT010000000
VERSION     DAMZFT010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12415)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12415)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12415
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.133"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(<1..756)
                     /locus_tag="PN977_004014"
     CDS             complement(<1..756)
                     /locus_tag="PN977_004014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDI6043993.1"
                     /translation="MTAINRILIVDDEDNVRRMLSTAFALQGFETHCANNGRTALHLF
                     ADIHPDVVLMDIRMPEMDGIKALKEMRSHETRTPVILMTAYAEVETAVEALRCGAFDY
                     VIKPFDLDELNLIVQRTLQLQSMKKEIRHLHQALSTSWQWGHILTNSPAMMDICKDTA
                     KIALSQASVLISGESGTGKELIARAIHYNSRRAKGPFIKVNCAALPESLLESELFGHE
                     KGAFTGAQTLRQGLFERANEGTLLLDEIGEMPLV"
     gene            complement(753..2579)
                     /gene="atoS"
                     /locus_tag="PN977_004015"
     CDS             complement(753..2579)
                     /gene="atoS"
                     /locus_tag="PN977_004015"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     AtoS"
                     /protein_id="HDI6043994.1"
                     /translation="MHYMKWIYPRRLRNQMILMAILMVIVPTLTIGYIVETEGRSAVL
                     SEKEKKLSAVVNLLNQALGDRYDLYIDLPREERIRALNAELAPITENITHAFPGIGAG
                     YYNKTLDAIITYAPSALYQNNVGVTIAADHPGREVMRTNTPLVYSGRQVRGDILNSMI
                     PIERNGEILGYIWANELTEDIRRQAWKMDVRIIIVLTAGLLISLLLIVLFSRRLSANI
                     DIITDGLSTLAQNIPTRLPQLPGEMGQISQSVNNLAQALRETRTLNDLIIENAADGVI
                     AIDRQGDVTTMNPAAEVITGYQRHELVGQPYSMLFDNTQFYSPVLDTLEHGTEHVALE
                     ISFPGRDRTIELSVTTSRIHNTHGEMIGALVIFSDLTARKETQRRMAQAERLATLGEL
                     MAGVAHEVRNPLTAIRGYVQILRQQTSDLIHQEYLSVVLKEIDSINKVIQQLLEFSRP
                     RHSQWQQVSLNALVEETLVLVQTAGVQARVDFISELDNELSPINADRELLKQVLLNIL
                     INAVQAISARGKIRIRTWQYSDSQQAISIEDNGCGIDLSLQKKIFDPFFTTKASGTGL
                     GLALSQRIINAHQGDIRVASLPGYGATFTLILPINPQGNQTV"
     gene            2746..5595
                     /gene="rcsC"
                     /locus_tag="PN977_004016"
     CDS             2746..5595
                     /gene="rcsC"
                     /locus_tag="PN977_004016"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RcsC"
                     /protein_id="HDI6043995.1"
                     /translation="MKYLASFRTTLKASRYMFRALALVLWLLIAFSSVFYIVNALHQR
                     ESEIRQEFNLSSDQAQRFIQRTSDVMKELKYIAENRLSAENGVLSPRGRETQTDVPAF
                     EPLFADSDCSAMSNTWRGSLESLAWFMRYWRDNFSAAYDLNRVFLIGSDNLCMANFGL
                     RDMPVERDTALKALHERINKYRNAPQDDSGSNLYWISEGPRPGVGYFYALTPVYLANR
                     LQALLGVEQTIRMENFFLPGTLPMGVTILDENGHTLISLTGPESKIKGDPRWMQERSW
                     FGYTEGFRELVLKKNLPPSSLSIVYSVPVDKVLERIRMLILNAILLNVLAGAALFTLA
                     RMYERRIFIPAESDALRLEEHEQFNRKIVASAPVGICILRTADGVNILSNELAHTYLN
                     MLTHEDRQRLTQIICGQQVNFVDVLTSNNTNLQISFVHSRYRNENVAICVLVDVSSRV
                     KMEESLQEMAQAAEQASQSKSMFLATVSHELRTPLYGIIGNLDLLQTKELPKGVDRLV
                     TAMNNSSSLLLKIISDILDFSKIESEQLKIEPREFSPREVMNHITANYLPLVVRKQLG
                     LYCFIEPDVPVALNGDPMRLQQVISNLLSNAIKFTDTGCIVLQVRADGDYLSIRVRDT
                     GVGIPAKEVVRLFDPFFQVGTGVQRNFQGTGLGLAICEKLISMMDGDISVDSEPGMGS
                     QFTVRIPLYGAQYPQKKGVEGLSGKRCWLAVRNASLCQFLETSLQRSGIVVTTYEGQE
                     PTPEDVLITDEVVSKKWQGRAVVTFCRRHIGIPLEKAPGEWVHSVAAPHELPALLARI
                     YLIEMESDDPANALPSTDKAVSDNDDMMILVVDDHPINRRLLADQLGSLGYQCKTAND
                     GVDALNVLSKNHIDIVLSDVNMPNMDGYRLTQRIRQLGLTLPVIGVTANALAEEKQRC
                     LESGMDSCLSKPVTLDVIKQTLTVYAERVRKSRES"
     gene            complement(5681..6331)
                     /gene="rcsB"
                     /locus_tag="PN977_004017"
     CDS             complement(5681..6331)
                     /gene="rcsB"
                     /locus_tag="PN977_004017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator RcsB"
                     /protein_id="HDI6043996.1"
                     /translation="MNNMNVIIADDHPIVLFGIRKSLEQIEWVNVVGEFEDSTALINN
                     LPKLDAHVLITDLSMPGDKYGDGITLIKYIKRHFPSLSIIVLTMNNNPAILSAVLDLD
                     IEGIVLKQGAPTDLPKALAALQKGKKFTPESVSRLLEKISAGGYGDKRLSPKESEVLR
                     LFAEGFLVTEIAKKLNRSIKTISSQKKSAMMKLGVENDIALLNYLSSVTLSPADKD"
     gene            complement(6348..9020)
                     /gene="rcsD"
                     /locus_tag="PN977_004018"
     CDS             complement(6348..9020)
                     /gene="rcsD"
                     /locus_tag="PN977_004018"
                     /EC_number="2.7.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase RcsD"
                     /protein_id="HDI6043997.1"
                     /translation="MRQKETTATTRFSLLPGSITRFFLLLIIVLLVTMGVMVQSAVNA
                     WLKDKSYQIVDITHAIQKRVDTWRYVTWQIYDNIAATTSPSSGEGLQETRLKQDVYYL
                     EKPRRKTEALIFGSHDNSTLEMTQRMSTYLDTLWGAENVPWSMYYLNGQDNSLVLIST
                     LPLKDLTSGFKESTVSDIVDSRRAEMLQQANALDERESFSNMRRLAWQNGHYFTLRTT
                     FNQPGHLATVVAFDLPINDLIPPGMPLDSFRLEPDATATGDNDNEKEGTDSVSIHFNS
                     TKIEISSALNSTDMRLVWQVPYGTLLLDTLQNILLPLLLNIGLLALALFGYTTFRHFS
                     SRSTESVPSTAVNNELRILRAINEEIVSLLPLGLLVHDQESNRTVISNKIADHLLPHL
                     NLQNITTMAEQHQGIIQATINNELYEIRMFRSQVAPRTQIFIIRDQDREVLVNKKLKQ
                     AQRLYEKNQQGRMTFMKNIGDALKEPAQSLAESAAKLNAPEGKQLTNQADVLVRLVDE
                     IQLANMLADDSWKSETVLFSVQDLIDEVVPSVLPAIKRKGLQLLINNHLKAHDMRRGD
                     RDALRRILLLLMQYAVTSTQLGKITLEVDQDESSEDRLTFRILDTGEGVSIHEMDNLH
                     FPFINQTQNDRYGKADPLAFWLSDQLARKLGGHLNIKTRDGLGTRYSVHIKMLAADPK
                     VEEEEERLLDDVCVMVDVTSAEIRNIVTRQLENWGATCITPDERLISQDYDIFLTDNP
                     SNLTASGLLLSDDESGVREIGPGQLCVNFNMSNAMQEAVLQLIEVQLAQEEVTESPLG
                     GDENAQLHASGYYALFVDTVPDDVKRLYTEAATSDFAALAQTAHRLKGVFAMLNLVPG
                     KQLCETLEHLIREKDVPGIEKYISDIDSYVKSLL"
     gene            9759..10853
                     /locus_tag="PN977_004019"
     CDS             9759..10853
                     /locus_tag="PN977_004019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HDI6043998.1"
                     /translation="MKVKVLSLLVPALLVAGAANAAEIYNKDGNKLDLYGKVDGLHYF
                     SDNDSKDGDKTYMRLGFKGETQVTDQLTGYGQWEYQIQGNEPESDNSSWTRVAFAGLK
                     FQDVGSFDYGRNYGVVYDVTSWTDVLPEFGGDTYDSDNFMQQRGNGFATYRNTDFFGL
                     VDGLDFAVQYQGKNGSAHGEGMTTNGRDDVFEQNGDGVGGSITYNYEGFGIGAAVSSS
                     KRTWDQNNTGLIGTGDRAETYTGGLKYDANNIYLAAQYTQTYNATRVGSLGWANKAQN
                     FEAVAQYQFDFGLRPSLAYLQSKGKNLGRGYDDEDILKYVDVGATYYFNKNMSTYVDY
                     KINLLDDNQFTRAAGINTDDIVALGLVYQF"
     gene            10965..12020
                     /gene="apbE"
                     /locus_tag="PN977_004020"
     CDS             10965..12020
                     /gene="apbE"
                     /locus_tag="PN977_004020"
                     /EC_number="2.7.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase ApbE"
                     /protein_id="HDI6043999.1"
                     /translation="MEISFTRVALLAAALFFVGCDQKPQPAKTHATEVTVLEGKTMGT
                     FWRASIPGIDAKRSAELKEKIQTQLDADDQLLSTYKKDSALMRFNDSQSLSPWPVSEA
                     MADIVTTSLRIGARTDGAMDITVGPLVNLWGFGPEQQPVQIPSQEQIDAMKAKTGLQH
                     LTVINQSHQQYLQKDLPDLYVDLSTVGEGYAADHLARLMEQEGISRYLVSVGGALNSR
                     GMNGEGQPWRVAIQKPTDKENAVQAVVDINGHGISTSGSYRNYYELDGKRLSHVIDPQ
                     TGRPIEHNLVSVTVIAPTALEADAWDTGLMVLGPDKAKEVVRREGLAVYMITKEGDSF
                     KTWMSPQFKSFLISEKN"
     gene            12094..>12415
                     /locus_tag="PN977_004021"
     CDS             12094..>12415
                     /locus_tag="PN977_004021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311129.1"
                     /note="regulates genes involved in the repair of alkylated
                     DNA; repairs DNA containing 6-O-methylguanine; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional transcriptional activator/DNA
                     repair enzyme protein Ada"
                     /protein_id="HDI6044000.1"
                     /translation="MKNATCLTDDQRWQSVLARDPNADGEFVFAVRTTGIFCRPSCRA
                     RHALRENVSFYANASEALAAGFRPCKRCQPDKANAQQHRLDKITHACRLLEQETPVTL
                     EALAD"
BASE COUNT         3053 a         2955 c         3162 g         3245 t
ORIGIN      
        1 taccagcggc atttcgccaa tttcgtcgag gagcagagta ccttcgttgg cacgttcaaa
       61 taatccctga cgtaaggttt gtgcaccagt aaatgcgcct ttttcatgac caaacagttc
      121 actttcgagc aacgattccg gcagtgccgc gcagttgact ttaatgaacg gcccctttgc
      181 cctccgcgaa ttgtagtgaa tcgctctggc aatcaactct ttcccggtgc cgctttcacc
      241 gctaatcaag acgctggcct gagaaagggc aattttggcg gtgtctttgc agatgtccat
      301 catcgccggg ctgttggtga gaatgtgccc ccattgccag ctggtgctca gtgcctggtg
      361 cagatgacgg atctcttttt tcattgactg gagttgtaaa gtgcgctgaa cgattaaatt
      421 caactcatcg agatcaaacg gtttgataac atagtcgaag gctccgcagc gtagtgcttc
      481 gacggcggtt tccacttcag cataggccgt catcagaata acgggtgtcc gggtctcatg
      541 gctgcgcatc tccttgagtg ccttgatgcc gtccatctct ggcatgcgga tatccatcaa
      601 caccacgtca gggtgaatat cggcaaacag gtgtaatgct gtgcgcccgt tgttcgcaca
      661 atgtgtttcg aatccttgta gtgcaaaagc ggtgctcagc atacggcgaa cattatcttc
      721 atcatccaca ataaggatgc gattaatagc agtcatacag tctgatttcc ctgcgggttg
      781 atcggtaaaa taagcgtgaa ggttgcgccg tagcccggca aactggcgac gcgaatatca
      841 ccctgatggg cattaatgat gcgttgactt aacgccagac caagcccggt tcctgaggct
      901 ttggtggtga aaaagggatc gaagatcttt ttttgcagcg agagatcaat gccacagccg
      961 ttgtcctcta tcgaaatggc ctgttgtgag tcgctgtatt gccaggttcg aatgcgaatt
     1021 ttccctcgtg cactgatagc ctggacggca ttgatcagga tattcagtag tacctgtttg
     1081 agcagttcac gatcggcgtt aatcgggctt aattcattat ccagttcgct tatgaagtcg
     1141 acccgcgctt gtacgccggc ggtttgtacc agtaccagag tttcttcaac caatgcattg
     1201 aggctgactt gttgccattg actgtggcgt ggacgtgaaa attcgagcaa ttgctgaata
     1261 actttgttaa ttgaatcaat ttctttgagt actacggaca gatattcctg atgtattagg
     1321 tcgctggttt gttggcgcaa gatctgtaca taaccacgaa tagccgttag cggattacgt
     1381 acttcatgcg ccacgccagc catcagctca cccagtgtgg cgaggcgttc tgcttgcgcc
     1441 atgcggcgct gggtttcttt gcgggcagtt aaatcagaga aaatcaccaa agcacctatc
     1501 atttcaccgt gcgtgttatg aatacgactg gtcgtgacac tgagttcaat ggtgcggtca
     1561 cggcctggaa aactgatctc cagcgccaca tgttcggtgc catgttccag cgtatccagt
     1621 actggactgt agaactgagt attgtcgaac aacatggagt aaggctgccc taccagttca
     1681 tgacgttgat agccagtgat gacttctgct gccgggttca tggtggttac atcaccctgg
     1741 cggtcaatgg caatgacgcc atcggcagcg ttttcaataa tcagatcgtt aagtgtccgc
     1801 gtttcacgca gtgcctgggc gaggttgtta acactctgac tgatttgccc catttcaccg
     1861 ggcaattgtg gtaatcgagt gggaatattt tgtgccagag tcgagaggcc atcggtgatg
     1921 atatctatat tggcgctcag gcgacgggag aaaaggacaa tcaacagcag gcttatcagc
     1981 aaaccggcgg tgagcacaat gataatcctc acatccattt tccaggcctg gcggcgaata
     2041 tcttcggtta attcattggc ccagatatag ccgaggattt caccattacg ctcaatggga
     2101 atcattgaat tcaaaatatc gccgcgcacc tgcctgcctg aataaaccaa aggggtattt
     2161 gtacgcatga cttcgcgacc agggtgatct gcggcaatgg taacgccgac attattctga
     2221 tatagcgctg aaggcgcgta ggtgattatc gcatccagcg ttttgttgta ataaccagcg
     2281 ccgatgccag ggaaggcgtg agtgatattt tcggtaatgg gggcaagttc tgcatttaat
     2341 gcgcggatac gctcctcacg tggtaagtcg atgtagagat catagcgatc gcctagtgcc
     2401 tgattaagca ggttgaccac ggcagaaagt tttttctctt tttcagataa gactgctgaa
     2461 cgtccttccg tttctacgat ataaccaata gtaagcgttg ggacaatgac catcaggatt
     2521 gccatcagga tcatttgatt gcgtaagcgg cgtggataaa tccacttcat ataatgcatg
     2581 ctgaaatcca ctagtcttgt ccggtatatg acgattatca gaggttaagg tgatgatttc
     2641 tcggcggtgt atcatattcc agagaagaga gaacattgcg gtaacacgct tttaccgcta
     2701 ccttaaccac actccatcgg tcacctgagg cggagcttcg cccctttgaa ataccttgct
     2761 tcttttcgta caaccctgaa agcctcgcgc tacatgttca gagcattggc gttagtgctc
     2821 tggctgttga ttgctttttc atccgttttt tacatcgtta atgcgttaca tcagcgagaa
     2881 tcggaaattc gtcaggaatt taatctgagt tccgatcagg ctcagcgctt tattcaacgc
     2941 acctctgatg tgatgaaaga gctgaagtac atcgccgaaa atcgcttatc ggcagaaaac
     3001 ggtgtgcttt ccccgcgtgg acgagaaacg cagacggatg tgcctgcgtt tgaaccgctg
     3061 tttgctgact ctgattgttc cgcaatgagt aacacctggc gaggttctct ggagtcattg
     3121 gcgtggttta tgcgctactg gcgcgataat ttttctgcgg cttacgatct caaccgggta
     3181 tttttaatcg gcagcgataa cctctgcatg gccaatttcg gtctgcgtga tatgccagtg
     3241 gaacgcgata ccgcgctaaa agctttgcat gaacgcatta ataaatatcg aaatgcacca
     3301 caagatgata gtggcagtaa cctctactgg atcagcgaag gtccgcgccc tggcgtcggg
     3361 tatttttacg cgttgacgcc agtttatctg gcgaaccggt tgcaggcgct tttgggtgtc
     3421 gagcagacca tccggatgga gaactttttc ttgcctggta cgttgccgat gggggttacc
     3481 attcttgatg aaaatggtca taccctgatt tcgcttaccg gaccagaaag caaaattaag
     3541 ggcgatcctc gctggatgca ggaacgctcc tggtttggct atacggaagg gttccgggag
     3601 ctggtgctga agaaaaatct gccaccctca tcgctaagca tcgtgtattc ggtgccggtt
     3661 gataaggtgc tggaacgcat tcgcatgttg atccttaacg caattttgct gaatgtgctt
     3721 gccggagctg cattgtttac tctcgcgcgg atgtacgagc gacgtatttt cattccggcg
     3781 gaaagcgacg ccctgcgact ggaagaacat gagcagttta atcgcaagat tgtcgcctcc
     3841 gcgccagtgg gtatctgcat tttgcgtacc gctgatggcg tcaatatttt aagtaacgaa
     3901 ctggcgcata cctatctcaa tatgcttacg catgaggatc gccaacggct gacacaaatt
     3961 atctgtgggc agcaggtcaa ttttgttgat gtcctgacca gcaacaatac caatctgcaa
     4021 atcagcttcg tccattcgcg ctatcgtaat gaaaacgtgg ccatttgtgt gctggtggat
     4081 gtttcttcgc gcgtgaagat ggaagagtcg ttgcaggaga tggcacaagc agcggaacag
     4141 gcgagtcagt caaaatcgat gttccttgcc accgtcagtc atgagctgcg aacgccgctg
     4201 tatggcatta tcggtaacct ggatctattg caaaccaaag agttgccgaa aggcgtcgat
     4261 cgtctggtga cggcaatgaa caactcttcc agcctgttgt tgaaaattat cagcgatatt
     4321 ctcgatttct cgaagattga atcagaacag ttgaagatcg aaccgcgtga gttttcaccg
     4381 cgtgaagtga tgaaccacat caccgccaac tatttaccgc tggtggtacg caagcagtta
     4441 ggcttgtact gctttattga accggatgtg ccagtggcct taaatggcga cccgatgcgt
     4501 ttacagcagg tcatctccaa cctgttgagt aacgccataa aattcaccga taccggctgt
     4561 atagttttgc aagttcgcgc tgatggcgat tatctctcta tccgtgttcg cgataccggc
     4621 gtggggattc cggcgaaaga agtcgtgcgc ttgtttgatc ccttcttcca ggtcggaacg
     4681 ggcgtacagc gaaacttcca ggggaccggt ctgggtctgg cgatttgtga aaaactgatc
     4741 agcatgatgg acggcgacat ctcggtagat tcagaaccgg gaatgggcag ccagtttacc
     4801 gtgcgtattc cgttgtacgg cgctcagtac ccgcagaaaa aaggcgtgga agggttgagt
     4861 ggtaaacgct gctggctggc ggtccgcaat gcgtcgctct gtcagttcct ggaaaccagt
     4921 ttgcagcgca gcggcatcgt cgttacaaca tacgaagggc aggaaccgac tcccgaagat
     4981 gtgttaatta ctgacgaggt agtgagtaaa aaatggcagg gtagagcggt agtgaccttc
     5041 tgtcgtcgcc atattggtat tccgctggag aaagcgccag gggagtgggt acacagtgtg
     5101 gcggccccgc atgagctacc ggcattgttg gcgcgtattt atttgatcga gatggagagc
     5161 gatgatcctg ctaacgctct gccgtcgacg gacaaagcgg tcagcgataa cgacgatatg
     5221 atgattctgg tcgtggatga tcatccgatt aaccggcgtc ttctggcaga tcagttggga
     5281 tcgttgggct atcaatgtaa aaccgcgaat gatggcgtcg atgcgcttaa tgtacttagc
     5341 aagaatcata ttgatatcgt gcttagcgac gtcaacatgc caaatatgga tggttaccgc
     5401 ttgacgcaac gcattcgtca gttgggactg acgttgccgg taatcggagt aactgctaat
     5461 gcgttggctg aagagaagca gcggtgtctg gagtccggta tggacagctg cctgtcgaag
     5521 ccggtaacgc tggatgtgat aaaacagacg ctgacggtat atgccgagag ggtcaggaaa
     5581 tcgcgggaat cgtaggacgg ataaggcgtt tacgccgcat ctggcattca gtgcaaatgc
     5641 cagataagac actaacgcgt cttatctggc ctacaggtga ttagtcttta tctgccggac
     5701 ttaacgtcac tgaagagaga taattcagca gggcgatatc gttctcgaca cccagcttca
     5761 tcatcgcaga tttcttctgg ctactgatgg ttttaatact gcggttcagc tttttagcga
     5821 tctcggtcac caggaagcct tccgcaaaca ggcgcagaac ttcactctct tttggcgaga
     5881 gacgcttgtc accgtaacca ccggcactga ttttttccaa caggcgagaa acgctttccg
     5941 gggtaaattt cttccctttc tgcagcgcgg cgagagcttt cggcagatcg gtcggtgcac
     6001 cttgtttcag cacgatccct tcgatatcca gatccaatac cgcactaaga atcgccgggt
     6061 tgttgttcat ggtcagaaca atgatcgaca ggcttgggaa atggcgcttg atgtacttga
     6121 ttaaggtaat gccatcgccg tacttatcgc caggcatgga gagatcggta atcaacacat
     6181 gcgcatccag tttcggcagg ttgttgatca gtgctgtaga gtcttcaaat tcgccgacaa
     6241 cattcaccca ctcaatttgc tcaagtgatt tgcgaatacc gaacaagact atcggatggt
     6301 catcggcaat aattacgttc atattgttca tgtaataggc taccttgcta cagcaagctc
     6361 ttgacataac tgtcaatgtc gctgatgtat ttttctattc ctggaacatc cttctcacga
     6421 atcagatgtt ccagcgtttc acataactgc ttgccgggta ccagatttag catggcaaat
     6481 acgcctttaa gacgatgagc cgtttgggct aacgcagcaa agtcactggt tgctgcttca
     6541 gtatacagcc tcttaacatc atccggtact gtgtctacaa agagcgcata atagccgctg
     6601 gcatggagtt gcgcattttc atctccgccc agaggcgatt ctgtcacctc ttcctgcgcc
     6661 agttgcactt caattaattg taagaccgct tcctgcatag cgttgctcat attgaagttg
     6721 acgcacaatt gaccaggccc aatttcccgt acgccagact catcatcgct taaaagcaag
     6781 ccagaggcag taagattaga cggattatcc gttaaaaaga tatcataatc ttgactaatt
     6841 aatctttcat cgggtgtgat acaggttgca ccccaatttt ctaactggcg agtgacaata
     6901 ttccgaattt ctgccgaagt aacatccacc attacgcaga catcatccag taatcgctct
     6961 tcttcctctt caactttcgg gtcagctgcg agcattttga tatgcacaga gtagcgtgta
     7021 ccaagcccat cccgcgtttt gatgtttaaa tgaccgccca gtttacgcgc cagttgatcg
     7081 ctcagccaga atgccagcgg gtccgccttg ccatagcgat cgttttgggt ctggttgata
     7141 aacgggaagt gcaaattatc catttcatga atacttacgc cttctcccgt gtccagaatg
     7201 cggaacgtca ggcggtcttc ggaggactca tcctgatcaa cctcaagggt gatttttccc
     7261 aattgcgttg aggtcacggc atattgcatc agtagcagca aaatacgtcg taaggcatcg
     7321 cgatcgccgc ggcgcatatc gtgtgctttc agatgattgt taatcagcag ttgcagacct
     7381 ttacgcttga tggcaggcaa cactgaaggc acaacttcat caattaaatc ctgcacggag
     7441 aacagcaccg tctcactttt ccagctatcg tccgcaagca tgttcgctaa ctgtatttca
     7501 tcgaccagcc gcaccagcac atccgcctga ttcgtcagtt gtttgccttc cggggcgttg
     7561 agtttagccg cgctctccgc cagggactgt gcgggttctt tcagcgcatc gccaatgttt
     7621 ttcataaagg tcatccgccc ctgctggttt ttctcataca gacgctgcgc ctgcttgagt
     7681 ttcttgttta ccagcacttc gcgatcctga tcgcgaataa tgaaaatttg tgtgcgcggc
     7741 gcaacctggc tgcggaacat gcggatctca tacagctcgt tattgatcgt cgcctgaata
     7801 atcccctgat gctgttccgc catggtggtg atgttttgca gattcaaatg cggcagcaaa
     7861 tgatcggcaa ttttgttact tatgactgtg cggttcgatt cctgatcgtg aaccagcagg
     7921 ccgagcggca gcagtgagac tatctcttca ttaattgccc gtaaaatgcg caattcgtta
     7981 ttgaccgccg tgctggggac gctttctgta ctgcggctgg agaaatggcg gaatgtggta
     8041 tagccaaata acgccagcgc cagcaaaccg atgttcagca gcagtggcag cagaatgttt
     8101 tgcaacgtat ccagcaataa ggtgccataa ggaacctgcc agaccagtcg catatcggta
     8161 gagttgagtg ccgaggagat ttcaatcttc gtactgttaa agtggatact gacgctatcc
     8221 gtcccttctt tctcattatc attgtctccc gttgccgtcg cgtctggctc aaggcggaaa
     8281 ctgtccagcg gcatgcccgg tgggatcaaa tcattaatcg gcagatcaaa agccacaacc
     8341 gttgccagat gtcccggctg gttaaaggtg gtacgtaagg taaagtaatg accgttctgc
     8401 caggccaggc ggcgcatgtt agaaaagctt tcgcgttcat cgagggcgtt ggcctgttgc
     8461 aacatctctg cacgacgtga atcaacaatg tcgctgacgg tcgattcttt aaatccggag
     8521 gtgagatctt tgaggggcag ggttgagatc agcaccagac tgttatcctg accattcagg
     8581 taatacatcg accacggtac attttctgcg ccccacaatg tgtccagata ggtggacatc
     8641 cgttgagtca tctcaagcgt tgagttgtcg tgagagccaa agattaacgc ttccgttttg
     8701 cggcgtggtt tctccagata gtagacatcc tgtttcaggc gcgtctcttg taaaccttcg
     8761 ccggaggagg gggaggtcgt cgcggcaatg ttgtcgtaga tctgccaggt cacgtaacgc
     8821 caggtatcga cgcgcttttg gatagcgtgg gtaatgtcga caatctggta acttttatct
     8881 ttcagccagg cgttaacagc gctttgtacc attactccca tcgtcaccag taacacaatg
     8941 atcaacagta aaaagaagcg ggtaatgctc cccggtagga gtgaaaagcg ggtcgtggcc
     9001 gttgtctctt tctgacgcat tcgtgtttat gacctgttaa aacttcgcga agtgataggg
     9061 cagtataaag ggtacagtgt gaaattccag actcttacct gattgatgat tctctattct
     9121 cccgtgatat ttatcatcct gcattggggg aaatagatgt acaagcgcca ttttgctgta
     9181 caaataacct acaaaaagcc caatgaaatt aattgcaaat aaacgaagat tgctggaaat
     9241 tatgctgatg ttaattattt gtgaaatagt taacaagcgt tatagttttt ctgtggtagc
     9301 acagaataat gaaaagtgtg taaagaaggg taaaaaaaac cggatgcgag gcatccggtt
     9361 gaaatagggg taaacagaca ttcagaaatg aatgacggta ataaataaag ttaatgatga
     9421 tagcgggaga tattctagtt gcgagtgaag gttttgtttt gacattcagt gctgtcaaat
     9481 acttaagaat aagttattga ttttaacctt gaattattat tgcttgatgt taggtgctta
     9541 tttcgccatt ccgcaataat cttaaaaagt tcccttgcat ttacattttg aaacatctat
     9601 agcgataaat gaaacatctt aaaagtttta gtatcatatt cgtgttggat tattctgcat
     9661 ttttggggag aatggacctg ccgactgatt aatgagggtt aatcagtatg cagtggcata
     9721 aaaaagcaaa taaaggcata taacagaggg ttaataacat gaaagttaaa gtactgtccc
     9781 tcctggtccc agctctgctg gtagcaggcg cagcaaacgc tgctgaaatt tacaacaaag
     9841 acggcaacaa attagatctg tacggtaaag ttgacggcct gcactatttc tccgataacg
     9901 actctaaaga tggcgacaag acctacatgc gtcttggctt caaaggtgaa actcaggtta
     9961 ctgatcaact gaccggttat ggtcagtggg aatatcagat tcaaggtaac gaacctgaaa
    10021 gtgacaatag ttcctggacc cgtgtggcat tcgctggtct gaaattccag gacgttggtt
    10081 cttttgacta tggtcgtaac tacggcgttg tttacgatgt tacttcctgg accgacgtac
    10141 tgccagaatt cggtggtgat acctacgatt ctgataactt catgcagcag cgtggtaacg
    10201 gtttcgcgac ctaccgtaac accgacttct tcggtctggt tgacggtctt gattttgctg
    10261 ttcagtatca gggtaaaaac ggtagtgctc atggcgaagg tatgaccact aatggccgtg
    10321 atgatgtatt tgagcagaac ggtgacggtg ttggtggttc tattacttac aattatgaag
    10381 gctttggtat cggtgctgca gtttccagct ccaaacgtac ctgggatcag aacaacactg
    10441 gcctgatcgg tactggcgac cgtgctgaaa cctacactgg tggtctgaaa tacgacgcta
    10501 acaacatcta cctggctgct cagtacaccc agacctacaa cgcaactcgc gtaggttccc
    10561 tgggttgggc gaacaaagca cagaacttcg aagctgttgc tcagtaccag ttcgacttcg
    10621 gtctgcgtcc gtccctggct tacctgcagt ctaaaggtaa aaacctgggt cgtggctacg
    10681 acgacgaaga tatcctgaaa tatgttgatg ttggtgcgac ctactacttc aacaaaaaca
    10741 tgtccaccta cgttgactac aaaatcaacc tgctggacga caaccagttc acccgcgctg
    10801 ctggcatcaa cactgatgac atcgtagctc tgggtctggt ttaccagttc taatctcgat
    10861 ttatatcgaa caaagggcct gcgggccctt ttttcattgt tttcagcgta caaacacagt
    10921 tttttggtgt actcttgcga ccgttcgcat gaggataatc acgtatggaa ataagcttta
    10981 cccgcgtggc actgctggct gccgcgctct tctttgttgg ttgcgatcaa aaaccacaac
    11041 ccgccaaaac ccacgctact gaagttaccg ttcttgaagg caaaaccatg ggtaccttct
    11101 ggcgtgccag catcccgggc attgatgcca aacgcagtgc cgaacttaaa gaaaagattc
    11161 agacccagct ggacgccgac gatcagctgc tttcgaccta taaaaaagat tccgcgctga
    11221 tgcgctttaa cgactcgcaa agtttgtcgc cgtggccggt aagtgaagcg atggccgata
    11281 tcgtcaccac ctcgctgcgc attggcgcaa ggaccgatgg tgcgatggat ataaccgtcg
    11341 ggccgctggt gaatctgtgg ggctttggcc cggaacaaca gccggttcaa attccgagtc
    11401 aggaacagat cgatgcgatg aaagccaaaa ccggcttaca gcacctgacg gtgattaatc
    11461 agtcccacca gcaatatctg caaaaagacc tgccggattt atatgtcgat ctctctaccg
    11521 tcggtgaagg ttatgcggcg gatcatctgg cacgcttgat ggagcaggaa gggatttccc
    11581 gctatctggt gtcggtgggc ggcgcgctga acagccgtgg tatgaacggt gaaggccagc
    11641 cgtggcgagt ggcgattcaa aaaccaaccg ataaagaaaa cgcggttcag gcggtggtgg
    11701 atatcaacgg ccacggtatc agcacctccg gcagctatcg caactattac gaactggacg
    11761 gcaaacgtct ttctcatgtt atcgatccgc aaaccgggcg tcccattgaa cataatctgg
    11821 tatccgtgac ggtgattgcc ccgacggcgc tggaagccga cgcctgggac acaggtttga
    11881 tggtgctcgg gccggataaa gccaaagaag ttgttcgccg ggaagggctg gcggtctata
    11941 tgatcaccaa agagggcgat agctttaaaa cctggatgtc gccgcagttt aaaagcttcc
    12001 ttatcagcga aaaaaattaa agcgcaagat tgttggtttt tgcgcgatgg tgaccgggca
    12061 gactaaaggc tatccttaac cagggagctg attatgaaaa acgccacatg cttaacagac
    12121 gatcaacgct ggcaatctgt cttagcccgc gacccgaatg ccgacggcga attcgttttc
    12181 gccgtgcgca ccacaggtat cttttgccgt ccgtcttgcc gcgccagaca tgccttgcgg
    12241 gaaaatgtct ccttctacgc aaatgccagc gaggcactcg ccgccggatt tcgcccctgc
    12301 aaacgttgtc agccagacaa agccaatgcc cagcaacatc gccttgataa aattacccac
    12361 gcctgccgac ttctggaaca ggaaacgcct gtaacgctgg aagccttagc cgacc
//
LOCUS       DAMZFT010000134        12406 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.134, whole genome shotgun sequence.
ACCESSION   DAMZFT010000134 DAMZFT010000000
VERSION     DAMZFT010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12406)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12406)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12406
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.134"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            158..2632
                     /locus_tag="PN977_004022"
     CDS             158..2632
                     /locus_tag="PN977_004022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013203973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toll/interleukin-1 receptor domain-containing
                     protein"
                     /protein_id="HDI6044001.1"
                     /translation="MIKAFLSHSSKDKEHYVRNVANWLGKENIIYDEFTFEEGERTLD
                     QIMEGLGESELFVLFISNSALESEWVKKEITESKKLLDEGKILKIFPIIIDNSINHED
                     QRIPDWLRKDYNLQPITRARTAASRIKNHLYKISWQKHPKLAKISSLFVGRNDKLEEF
                     EERINDYTKKKPTVIFASGLIGVGRRSFLSKALIKCNIQKNQITPYAIYLDRNESIED
                     FILKLNDLGITDLKESALSLSEKTIEEKQKIISNILNEAYKAKETIFFLDDGCIINYK
                     RELTPWFKMIIEEYNDINFPVLCIASRYRVNFANRPRDDRFYFIELPELNVKERRRLL
                     SQLLEIENIDDLDPSNFSSVCDLLTGLPEQVRFAIELIKDKNIIPFENKLPILSDFNN
                     DKASIQLQRYAHNETVLDFIRLLAQFEVITLDFIFSIVDKDKYLPVLEALIAESICEL
                     VGSDGEMIRLNDIVRDYIKRNRLKINDEHNKKLNLVVKDIVNNEDLTEANSSVYIFTI
                     KEMLKSDEYIDPKHLIPSHYLRCMKDLYNTKGSLEKVISLADTILQKEKNMDNGVVQD
                     IRYYLCLALAKKSDSRLLTEVQKISGDEHKFLLGFYYRKCGRLSDALFQFESIINAPY
                     VESRAKRELVQVYTQLEEYDKALSYAKKNYEENRSNQFHCQAYFNCLINSQHRVASDN
                     IVLKDIITTLINIDSEQSNEMAKIADAMYSAKIENDAVKSITIINDCIAIYPESPYPL
                     LAKCDIGLKFKSIQTLEEALSSLDKMKRRKVVSERTLTTYLAYLMALKGDADSANQII
                     SNDIKKYPQEARERILKRIKEYAAKN"
     gene            complement(2708..3961)
                     /locus_tag="PN977_004023"
     CDS             complement(2708..3961)
                     /locus_tag="PN977_004023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000772682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase arm-type DNA-binding domain-containing
                     protein"
                     /protein_id="HDI6044002.1"
                     /translation="MKLNARQVETAKPKEKTYKMADGGGLYLEVSAKGSKYWRMKYRR
                     PSDKKEDRLAFGVWPTVTLAQARAKRDEAKKLLVQGIDPKTEQKQAQAENSGAYTFET
                     IAREWHASNKRWSEDHRSRVLRYLELYIFPHIGSSDIRLLKTSHLLAPIKKVDASGKH
                     DVAQRLQQRVTAIMRYAVQNDYIDSNPASDMAGALSTTKVRHYPALPSSRLPEFLSRL
                     AAYRGRIMTRIAVELSLLTFVRSSELRFARWNEFDFDKALWRVPAKREEIKGVRYSYR
                     GMKMKEEHIVPLSRQAMNLLGQLKQISGDRKLLFPGDHDATKVMSENTVNNALRAMGY
                     DTKTEVCGHGFRTMARGALGESGLWSDDAIERQLSHSERNNVRAAYIHTSEHLDERRL
                     MMQWWADYIDHNKIMYTTPFDFAKK"
     gene            complement(4125..4209)
                     /locus_tag="PN977_004024"
     tRNA            complement(4125..4209)
                     /locus_tag="PN977_004024"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4173..4175),aa:Leu,seq:caa)
     gene            4405..5424
                     /gene="ahr"
                     /locus_tag="PN977_004025"
     CDS             4405..5424
                     /gene="ahr"
                     /locus_tag="PN977_004025"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent aldehyde reductase Ahr"
                     /protein_id="HDI6044003.1"
                     /translation="MSMIKSYAAKEAGGELELYEYDPGELKPQDVEVQVDYCGICHSD
                     LSMIDNEWGFSQYPLVAGHEVIGRVVALGSAAQDKGLQVGQRVGIGWTARSCGHCDAC
                     ISGNQINCEQGAVPTIMNRGGFAEKLRADWQWVIPLPENIDIESAGPLLCGGITVFKP
                     LLMHHITATSRVGVIGIGGLGHIAIKLLHAMGCEVTAFSSNPAKEQEVLAMGADKVVN
                     SRDPQALKTLAGQFDLIINTVNVSLDWQPYFEALTYGGNFHTVGAVLTPLSVPAFTLI
                     AGDRSVSGSATGTPYELRKLMRFAARSKVAPTTELFPMSKINDAIQHVRDGKARYRVV
                     LKADF"
     gene            complement(5428..5991)
                     /gene="idnK"
                     /locus_tag="PN977_004026"
     CDS             complement(5428..5991)
                     /gene="idnK"
                     /locus_tag="PN977_004026"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HDI6044004.1"
                     /translation="MAGESFILMGVSGSGKTLIGSKVATLLSAKFIDGDDLHPAKNID
                     KMSQGIPLSDEDRLPWLERLNDASYSLYKKNETGFIVCSSLKKQYRDILRKGSPHVHF
                     LWLDGDYETILARMQRRAGHFMPVASLKSQFEALERPQADEQDIVRIDINHDIANVTE
                     QCRQAVLAIRQDRICAKGGSASDQRCE"
     gene            6208..7239
                     /gene="idnD"
                     /locus_tag="PN977_004027"
     CDS             6208..7239
                     /gene="idnD"
                     /locus_tag="PN977_004027"
                     /EC_number="1.1.1.264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-idonate 5-dehydrogenase"
                     /protein_id="HDI6044005.1"
                     /translation="MQVKTQSCVVAGKKTVAVTEQTIDWNNNGTLVQITRGGICGSDL
                     HYYQEGKVGNFMIKAPMVLGHEVIGKVIHSDSSELHEGQTVAINPSKPCGHCKYCIEH
                     NENQCTDMRFFGSAMYFPHVDGGFTRYKMVETSQCVPYPAKADEKVMAFAEPLAVAIH
                     AAHQAGELQGKRVFISGVGPIGCLIVSAVKTLGAAEIVCADVSPRSLSLGKEMGAEVL
                     VNPQNDDMDHWKAEKGYFDVSFEVSGHPSSVNTCLEVTRARGVMVQVGMGGAMAEFPM
                     MTLIGKEISLKGSFRFTSEFNTAVSWLANGVINPLPLLSAEYPFTDLEEALRFAGDKT
                     QAAKVQLVF"
     gene            7263..8027
                     /gene="idnO"
                     /locus_tag="PN977_004028"
     CDS             7263..8027
                     /gene="idnO"
                     /locus_tag="PN977_004028"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HDI6044006.1"
                     /translation="MNDLFSLAGKNILITGSAQGIGYLLATGLGKYGAQIIINDITAE
                     RAELAVEKLHQEGIQAVAAPFNVTHKHEIDAAVEHIEKDIGPIDVLVNNAGIQRRHPF
                     TEFPEQEWNDVIAVNQTAVFLVSQAVTRHMVERKAGKVINICSMQSELGRDTITPYAA
                     SKGAVKMLTRGMCVELARHNIQVNGIAPGYFKTEMTKALVEDEAFTAWLCKRTPAARW
                     GDPQELIGAAVFLSSKASDFVNGHLLFVDGGMLVAV"
     gene            8092..9411
                     /gene="idnT"
                     /locus_tag="PN977_004029"
     CDS             8092..9411
                     /gene="idnT"
                     /locus_tag="PN977_004029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gnt-II system L-idonate transporter"
                     /protein_id="HDI6044007.1"
                     /translation="MPLIIIAAGVALLLILMIGFKVNGFIALVLVAAVVGFAEGMDAQ
                     AVLHSIQNGIGSTLGGLAMILGFGAMLGKLISDTGAAQRIATTLIATFGKKRVQWALV
                     ITGLVVGLAMFFEVGFVLLLPLVFTIVASSGLPLLYVGVPMVAALSVTHCFLPPHPGP
                     TAIATIFEANLGTTLLYGFIITIPTVIVAGPLFSKLLTRFEKAPPEGLFNPHLFSEEE
                     MPSFWNSIFAAVIPVILMAIAAVCEITLPKTNTVRLFFEFVGNPAVALFIAIVIAIFT
                     LGRRNGRTIEQIMDIIGDSIGAIAMIVFIIAGGGAFKQVLVDSGVGQYISHLMTGTTL
                     SPLLMCWTVAALLRIALGSATVAAITTAGVVLPIINVTHADPALMVLATGAGSVIASH
                     VNDPGFWLFKGYFNLTVGETLRTWTVMETLISIMGLLGVLAINAVLH"
     gene            9478..10476
                     /gene="idnR"
                     /locus_tag="PN977_004030"
                     /gene_synonym="gntH"
                     /gene_synonym="yjgS"
     CDS             9478..10476
                     /gene="idnR"
                     /locus_tag="PN977_004030"
                     /gene_synonym="gntH"
                     /gene_synonym="yjgS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator IdnR"
                     /protein_id="HDI6044008.1"
                     /translation="MRNHRISLQDIATLAGVTKMTVSRYIRSPKKVAKETGERIAKIM
                     EEINYIPNRAPGMLLNAQSYTLGILIPSFQNQLFADILAGIESVTSVHNYQTLIANYN
                     YDRDSEEESVINLLSYNIDGIILSEKYHTIRTVKFLRSATIPVVELMDVQGERLDMEV
                     GFDNRQAAFDMVCTMLDKRVRRKILYLGSKDDTRDEQRYQGYCDAMMLHNLSPLRMNP
                     RAISSIHLGMQLMRDALSANPDLDGVFCTNDDIAMGALLLCRERNLAVPEQISIAGFH
                     GLEIGRQMIPSLASVITPRFDIGRMAAQMLLSKIKNNDHNHNTVDLGYQIYHGNTL"
     gene            10554..12056
                     /locus_tag="PN977_004031"
     CDS             10554..12056
                     /locus_tag="PN977_004031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF853 domain-containing protein"
                     /protein_id="HDI6044009.1"
                     /translation="MSEPLLIARTPDTELFLLPGMANRHGLITGATGTGKTVTLQKLA
                     ESLSEIGVPVFMADVKGDLTGVEQAGTASEKLLARLKNIGVNDWQPHANPVVVWDIFG
                     EKGHPVRATVSDLGPLLLARLLNLNDVQSGVLNIIFRIADDQGLLLLDFKDLRAITQY
                     IGDNAKSFQNQYGNISSASVGAIQRGLLSLEQQGAAHFFGEPMLDIKDWMRTDANGKG
                     VINILSAEKLYQMPKLYAASLLWMLSELYEQLPEAGDLEKPKLVFFFDEAHLLFNDAP
                     QVLLDKIEQVIRLIRSKGVGVWFVSQNPSDIPDNVLGQLGNRVQHALRAFTPKDQKAV
                     KAAAQTMRANPAFDTEKAIQELGTGEALISFLDAKGSPSVVERAMVIAPCSRMGPVTE
                     DERNGLINHSPVYGKYEDEVDRESAYEMLQKGFQASTEQQNNPPVKGKEVAVDDGILG
                     GLKDILFGTTGPRGGKKDGVVQTMAKSAARQVTNQIVRGMLGSLLGGRKR"
     gene            complement(12175..>12406)
                     /locus_tag="PN977_004032"
     CDS             complement(12175..>12406)
                     /locus_tag="PN977_004032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313266.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HDI6044010.1"
                     /translation="VAVMMLMALSFIFGPLRSVPMGVRVVTGISFGFVFYVLDQIFGP
                     LTLVYGIPPIIGALLPSASFFLISLWLLMRKS"
BASE COUNT         3477 a         2716 c         2914 g         3299 t
ORIGIN      
        1 gcactgcgcg caatctctga gcacgtttag tgttagatgt cacagcagca tattaactca
       61 ttaattaatc gttgaggttt caaataaatt gttatagatc ttaatctgaa ttgtctattc
      121 attcttagct aacgaataat tcccaaggag tattaaaatg ataaaggcat ttttatcgca
      181 ctcaagtaaa gataaggaac attatgtgcg taatgtcgct aattggcttg gcaaggaaaa
      241 cataatatat gatgaattta cttttgagga aggagaaaga acactagacc aaattatgga
      301 gggtttagga gaatcagagt tatttgttct ttttatttcc aacagcgcac ttgagtccga
      361 atgggtcaaa aaggaaataa ctgagtcaaa aaaactcctc gatgaaggta aaattttgaa
      421 aattttcccc ataataattg acaacagtat aaatcatgaa gaccaaagaa tacctgattg
      481 gttaagaaaa gactataatc ttcaacccat cacacgcgca agaactgcag cctcaagaat
      541 taagaaccat ctatacaaaa tttcttggca aaaacaccca aaacttgcca aaataagctc
      601 attatttgta ggaagaaacg ataagctaga agaatttgaa gagagaatta acgattacac
      661 taaaaaaaaa ccaactgtta tcttcgcatc tggtttaata ggtgtaggaa gaaggtcatt
      721 cttatctaaa gcgttaataa aatgtaacat tcaaaaaaat caaatcacac cttatgctat
      781 atatttagac agaaatgagt caattgagga ttttatccta aaacttaacg atttaggtat
      841 taccgatctt aaagaaagtg ctctatcact ttcagaaaaa acaattgagg aaaagcaaaa
      901 aataatatcc aacatattaa atgaagctta caaagcaaaa gaaacaatat ttttcctaga
      961 tgatgggtgt ataataaact ataagcgaga attaacccct tggtttaaaa tgataattga
     1021 agagtataat gacatcaatt tccctgtatt atgtatcgcc tcaagataca gggtcaactt
     1081 tgctaacagg cctcgtgatg acagatttta tttcattgaa ttacccgagc ttaatgtaaa
     1141 agaaagaagg cgtctattat cacaactatt agaaattgaa aatattgatg atctagaccc
     1201 atccaacttt agcagcgtct gcgacttact tactggtttg ccagaacaag taaggtttgc
     1261 tattgaactg ataaaggata aaaacataat accatttgag aataaacttc ctatactgtc
     1321 agattttaat aatgataaag catcaataca gctacaaagg tatgcccaca atgaaacggt
     1381 tttagacttt ataagactcc tcgctcaatt tgaggtaata acattagact ttattttttc
     1441 aatcgttgat aaggacaaat atctacctgt ccttgaagct ttgattgcag aaagcatttg
     1501 cgaattagtg ggttctgatg gtgaaatgat cagattaaat gatatcgtaa gagattatat
     1561 aaaaagaaac agacttaaga ttaatgatga gcataataaa aaacttaatt tagttgttaa
     1621 agatatagta aacaatgaag atttaacaga agcaaactca tctgtatata tttttaccat
     1681 aaaagaaatg ctcaaatcag atgagtatat tgatcctaaa caccttattc cttcgcatta
     1741 tttaagatgt atgaaagatt tatataatac aaaaggttca ttagaaaaag taatatcatt
     1801 agcagacaca attttacaaa aagaaaagaa catggataat ggtgttgtac aagatataag
     1861 atactatcta tgtcttgctt tagcaaaaaa atccgattcc aggcttttaa ctgaagttca
     1921 aaaaatttcc ggtgatgaac ataaattttt attgggtttc tattacagaa aatgcggaag
     1981 actttctgat gcactttttc agtttgaaag tataatcaat gctccatatg ttgagtctcg
     2041 agctaaacga gaattggttc aggtttatac acaattagaa gaatatgata aagcgttaag
     2101 ttatgctaaa aagaactatg aagaaaatag aagcaatcaa ttccactgtc aagcctattt
     2161 taattgtctc ataaattctc agcatagggt agcgtcagat aatatcgtgt taaaagatat
     2221 tatcaccacc ttaatcaaca ttgattcaga gcaatcaaat gaaatggcaa aaatagctga
     2281 cgcaatgtat tcagctaaaa ttgaaaatga tgcagtgaag tcaatcacca taataaatga
     2341 ctgtatagca atatatccag aaagtcctta tccattactc gcaaagtgtg atattggatt
     2401 aaaattcaag tctatacaga cactagagga agccttatcc tcattagata aaatgaaaag
     2461 gcgaaaagtt gtgtctgaac gaacacttac aacctatctg gcttatttaa tggctttaaa
     2521 gggtgatgca gatagtgcaa atcagataat atccaatgat attaaaaaat atcctcagga
     2581 agcaagagaa agaattttga aaaggataaa agaatatgcc gctaaaaatt aattaaaaat
     2641 ataacgcgat ggagaaaatt aaattttcct tcatcgcaat catgaataaa aaaatatcaa
     2701 ataataatta tttttttgca aaatcaaacg gagttgtata cataatctta ttatgatcta
     2761 tataatctgc ccaccactgc atcattaaac gacgttcatc caaatgctca gaagtatgga
     2821 tataagctgc acgcacatta ttacgctctg agtgactcaa ttgacgctca atagcatcat
     2881 cactccacaa acccgactcc cctaatgccc ctcgagccat cgtcctaaac ccatgcccac
     2941 agacttcggt tttagtgtca tatcccatcg cacgcaatgc gttgtttact gtgttttcac
     3001 tcataacctt agttgcgtca tgatcgccag ggaaaagcag tttcctatca ccactaatct
     3061 gctttaactg gcctaacaaa ttcatcgcct gacgactaag cggaacaata tgctcctctt
     3121 tcatcttcat accacgatac gagtaacgca cccccttaat ttcctctcgt tttgcaggta
     3181 cgcgccaaag ggctttatcg aagtcgaact cattccaacg cgcgaaacgt aactcactgg
     3241 aacgcacaaa agtcagtaag gagagctcga ctgcgatccg tgtcattata cgaccacgat
     3301 atgcagcgag acgtgaaaga aactcaggta accggctgga aggtaacgca gggtaatgtc
     3361 gcactttggt tgtagataac gcaccagcca tatcactggc cggatttgag tcgatgtaat
     3421 cgttttgtac agcataacgc ataatggccg ttacacgctg ttgcaggcgc tgagcgacgt
     3481 cgtgttttcc actggcgtca acttttttaa ttggggctaa caggtggctg gttttgagta
     3541 gacgaatatc ggacgaacca atatgaggga agatataaag ctcaaggtat cgaagaacgc
     3601 gcgatcgatg gtcttcgctc catcgcttgt tgctggcatg ccattcacga gcaatggttt
     3661 cgaaagtata tgcccccgaa ttttcggcct gagcttgttt ctgttcagtt ttcggatcta
     3721 taccctgcac taagagcttt ttagcttcat cgcgctttgc tcttgcctga gcaagcgtca
     3781 cagtaggcca aacaccaaaa gcaaggcgat cctctttttt gtcagagggg cgtctgtatt
     3841 tcatacgcca gtatttggaa cccttggccg aaacctcaag atacaaaccg ccgccatcgg
     3901 ccattttgta ggttttttct tttggctttg cggtttcgac ctgtctggca ttgagcttca
     3961 tttgggggca catttctaat cgaagttaag atgcccccaa ttatgccccc aatgacatcc
     4021 ggatttcaac ggactatctc ggacaactca gaacgtaaaa attgcttcaa gcattgattt
     4081 taaagggata attggacttt ctcggatggt cttggaaaga ctaatggtgc cgaaggccgg
     4141 actcgaaccg gcacgtattt ctacggttga ttttgaatca actgcgtcta ccgatttcgc
     4201 cacttcggca ctgaagaggt atgcggaaaa cgttgtggat tatacctgtc gcacgccacc
     4261 atgcaagcgc cagcttgctc gcatctcgct aagtgctgaa attttcagca ttacactcag
     4321 cgatgtgtaa cctttgtcac actccaggca ccccgccctg ccatgctcta cacttcccag
     4381 acaacaccag agaaggacca aaaaatgtcg atgataaaaa gctatgccgc aaaagaagcg
     4441 ggcggcgaac tggaacttta tgagtacgat cccggtgaat tgaagccaca agatgttgaa
     4501 gtgcaggtgg attactgcgg gatctgccat tccgatctgt cgatgatcga caacgaatgg
     4561 ggattttcac agtatccgct ggttgccggg catgaggtga ttggtcgcgt ggtggcgctc
     4621 gggagtgccg cacaggataa aggtttgcag gtcggtcagc gtgtcgggat tggctggacg
     4681 gcacgcagct gcgggcattg cgatgcctgt attagcggta atcagatcaa ctgcgagcaa
     4741 ggtgcggtgc caacaattat gaatcgcggc ggttttgccg agaagttgcg tgcggactgg
     4801 caatgggtga ttccgctgcc agaaaatatc gatatcgagt ccgccgggcc gctgctgtgt
     4861 ggtggtatca cggtctttaa accactgttg atgcaccata tcactgctac cagccgcgtt
     4921 ggggtaattg gtattggcgg gctggggcat atcgctataa aacttctgca cgcaatggga
     4981 tgcgaggtga cagcctttag ttctaatccg gcgaaagagc aggaagtact ggcgatgggt
     5041 gccgataaag tggtgaatag ccgcgatccg caggcactga aaactctggc ggggcagttt
     5101 gatctcatta tcaatactgt gaacgtcagc ctcgactggc agccttattt tgaggcgctg
     5161 acgtatggcg gtaatttcca tacggtcggt gcggttctca cgccgctgtc tgttccggcc
     5221 tttacgttaa ttgcgggcga ccgcagcgtc tctggctctg ctaccggcac gccttatgag
     5281 ctgcgtaagc tgatgcgctt tgccgcccgc agcaaggttg cgccaactac cgaactgttc
     5341 ccgatgtcga aaattaacga cgccatccag catgtgcgcg acggtaaggc gcgttaccgc
     5401 gtggttctga aagccgattt ttgaaaatca ttcgcagcgc tgatctgagg cgctgccccc
     5461 tttcgcacat attctgtctt gtcgtatcgc cagcacggcc tgccgacatt gttcggtgac
     5521 attggcaata tcatggttga tatcaatgcg cacaatatcc tgttcatctg cttgtggacg
     5581 ctccagcgcc tcaaactgac tttttagtga cgctaccggc ataaaatgcc cagcccgccg
     5641 ctgcattcgc gcgagaatag tttcatagtc gccatctaac cagaggaaat gaacatgggg
     5701 gctacccttg cgtaaaatat cacgatactg tttttttaat gatgaacaga caataaatcc
     5761 tgtttcattc tttttataaa gactgtatga agcatcattt aagcgttcca gccagggaag
     5821 tcgatcttca tcagataatg gaataccctg cgacatttta tctatatttt tggctggatg
     5881 aagatcgtca ccatcaataa atttagcaga taataacgtg gcaaccttgc taccaattaa
     5941 tgttttacca ctccctgaaa cgcccatcaa aataaagctt tcacccgcca tttaaaatct
     6001 cctgagtcat cttacctgct gcgatactac gcctggtggc ataataattt cacgtttttt
     6061 tatttctgct gataagaatt acaaggcaca tcacgttatg cgtaacatag taatgtaaca
     6121 attttctgac gtgatcttca tcacaaataa tgacaattaa aaccgcttaa atgcttccga
     6181 ggtgtctacc tgaccagtga ggaatttatg caagtgaaaa cacagtcctg cgttgttgcg
     6241 ggcaagaaaa ctgttgccgt taccgagcag acgatagatt ggaataataa tggaacatta
     6301 gtacaaataa cccgaggtgg aatttgcggt tccgatttac attattatca ggaaggaaaa
     6361 gtaggtaatt tcatgataaa ggcaccgatg gtgttaggtc atgaagttat cggtaaagtt
     6421 attcatagcg actcatcaga attacatgaa gggcaaacgg tagccattaa tccgtctaaa
     6481 ccgtgcggtc actgcaaata ctgcattgaa cataacgaga atcagtgtac agatatgcgt
     6541 ttttttggca gtgccatgta tttccctcat gttgatggtg gttttacccg ttataaaatg
     6601 gtcgaaacgt cgcaatgtgt cccttatccg gccaaagctg acgaaaaggt tatggctttt
     6661 gccgaacctt tagccgtcgc gattcatgcc gcacatcagg ccggcgagtt acagggcaag
     6721 cgagtattta tttctggtgt tgggcccatt ggctgcctga ttgtcagtgc tgtgaaaaca
     6781 ctgggggccg cggaaattgt ctgtgccgat gtgagtcccc gttccctttc gctgggcaaa
     6841 gagatggggg cggaggtgct cgtaaaccca caaaacgacg acatggatca ctggaaagcg
     6901 gaaaaaggct atttcgatgt cagctttgaa gtgtccggtc atccttcatc agtgaatacc
     6961 tgcctggagg tcactcgtgc acgcggcgta atggtgcagg tagggatggg aggcgcgatg
     7021 gcagaattcc caatgatgac gttgattggt aaggagattt cactcaaagg ctctttccgt
     7081 tttaccagcg aatttaatac cgcagtgtca tggctggcga atggcgttat caatccactg
     7141 cctttactga gtgctgaata tcccttcact gacctggaag aggcgctacg tttcgccggt
     7201 gataaaaccc aggcagcaaa agtccagctt gttttctaag agataaataa aggaataata
     7261 caatgaacga tctattttca ctggcaggaa aaaatatctt gattaccggt tcagcacagg
     7321 gcattggcta tttactggca accggcctgg gtaaatatgg cgcacaaata attattaatg
     7381 atattactgc cgaacgcgca gaacttgctg tagaaaaact ccaccaggag ggtattcagg
     7441 ccgttgccgc accttttaat gttactcata aacatgaaat tgatgccgcc gttgaacata
     7501 tcgaaaagga catcggcccc attgatgtgc tggtgaataa cgccggtatc cagcgccgtc
     7561 atccttttac tgagttccct gaacaagagt ggaatgatgt gatcgcagta aaccagaccg
     7621 ccgtgttcct ggtatcgcaa gcggtaactc gtcacatggt tgaacgcaag gcaggtaaag
     7681 ttattaatat ttgctcgatg caaagcgaac tgggacgtga caccatcacc ccttatgccg
     7741 catcgaaagg ggcggtaaaa atgctcaccc gcggcatgtg cgtcgagctg gcgcgccaca
     7801 atattcaggt caacggtatt gcgccgggct atttcaaaac agaaatgact aaagcactgg
     7861 ttgaggacga agccttcacc gcctggttgt gcaaacggac ccccgccgca cgctggggag
     7921 atccgcagga actgattggt gctgcggtgt tcctttcttc aaaagcctct gatttcgtaa
     7981 acggccacct gttgtttgtt gatggcggca tgttagtggc tgtttaacac ctactaccct
     8041 acgtgcagcg cgaaagcgct gcttttctgg cactaataag agataaagac tatgccatta
     8101 atcattattg cggcaggcgt cgcgctgctt cttatcctga tgatcggctt taaagttaac
     8161 ggctttattg ccctcgttct ggtcgctgcc gtcgtcggat ttgccgaagg gatggatgca
     8221 caggccgtcc tgcactctat acaaaatggt atcggcagca cgctcggcgg gctggcaatg
     8281 atcctcggtt tcggggccat gttaggcaag ctgatttctg atacgggcgc ggcacaacgt
     8341 atcgccacta cgctgattgc tacttttggt aaaaaacgcg tgcaatgggc gctagtgatc
     8401 accggtctgg ttgtcggcct cgccatgttt tttgaagtgg gttttgtcct gctgctgccg
     8461 ttggtattta ccatcgtggc atcatctgga ttacccctgt tgtatgttgg cgtaccgatg
     8521 gtagcagcgc tctctgtaac ccactgtttt ctgccgccac atccagggcc tactgccatc
     8581 gcgactatct ttgaggctaa tctcggaacg actctactgt atggatttat cattaccatt
     8641 ccgacagtta ttgtcgcagg accgctgttt tctaaactgc taactcgctt tgagaaagca
     8701 ccaccggaag gcttatttaa tcctcatctg tttagcgaag aggagatgcc ctccttctgg
     8761 aacagtattt tcgctgcagt gatcccggtc atcctgatgg ctatcgccgc cgtttgtgaa
     8821 attacgttac cgaaaactaa caccgtgcgc ctcttctttg aatttgtcgg taaccctgcc
     8881 gttgcgctgt ttattgccat tgttattgcg attttcacac tgggccgacg taatggacgc
     8941 accatcgagc aaatcatgga tatcattggg gattctatag gcgctatcgc gatgattgtg
     9001 tttattatcg ctggcggcgg cgcgtttaag caggtattag tagatagcgg tgtcgggcag
     9061 tatatttcac acttaatgac cggaactaca ctttcgccgt tattgatgtg ctggactgtt
     9121 gcggcgctgt tgcgtatcgc tctgggctct gccaccgtcg cggccattac caccgcgggt
     9181 gtggtgttgc cgattatcaa cgttacccat gccgatcccg ctttaatggt actggcaact
     9241 ggtgcgggca gcgtgatcgc gtcacacgta aacgaccctg gcttctggct atttaaaggg
     9301 tattttaatc tgacggttgg tgaaacgttg cgtacctgga cggtgatgga aacccttatt
     9361 tctattatgg gtttgctggg cgtgttagcc attaacgccg tattgcactg aaagaataaa
     9421 atattgtgat gtagtttcgg gggaagattg aagattcccc cgacgttggg agaagttatg
     9481 cgcaatcaca gaatttcttt acaggatatc gctacgctgg ctggcgtaac aaaaatgacc
     9541 gtgagtcgtt atatccgctc gccgaaaaag gtggcaaagg aaacaggcga gcgcatcgcc
     9601 aaaattatgg aagagatcaa ttatatccct aatcgcgctc ccggcatgtt gttgaatgca
     9661 caaagttata ccctcggtat cttgattccg tcatttcaaa atcaactctt cgctgacatt
     9721 ctcgcgggaa tagaatccgt cacctctgtg cataattatc agacccttat tgctaattac
     9781 aattacgatc gcgattccga agaagagtcg gtgattaacc tgctctctta taacattgac
     9841 gggattattc tttcggaaaa atatcacacc atcaggacgg tgaaatttct gcgttcagcc
     9901 accattccgg tcgtggaatt gatggatgta cagggagaac ggctggatat ggaggtcggt
     9961 tttgataatc ggcaggctgc ttttgacatg gtgtgtacca tgctggataa gcgagtcagg
    10021 cgcaaaattc tgtatctcgg ttccaaagat gacacccgcg atgaacagcg ttatcagggg
    10081 tattgcgatg cgatgatgct gcataatctt tccccattac gcatgaatcc acgcgccatc
    10141 tcatccattc atttaggaat gcagttaatg cgtgatgccc ttagcgcaaa cccggattta
    10201 gacggtgtat tttgcaccaa tgacgatatt gcgatgggtg cgcttctgct gtgccgcgag
    10261 cgaaacctcg ccgtgccaga acaaatttcc attgcgggtt ttcatgggct ggagattggc
    10321 aggcaaatga tcccaagcct cgccagcgta attactcccc gttttgatat tggtcggatg
    10381 gccgcccaaa tgttgctgag caaaattaag aataacgatc acaaccacaa cactgttgac
    10441 ctcgggtatc agatttatca cggtaacacg ctttaggtta ttttctcttc attacacata
    10501 ttctctttgc cggacgttgc ctatactcca ggcaggaaac tggaggaaat ctcatgagcg
    10561 aacccctgtt aattgcccgc acgccggaca cagaactgtt tttactgccg ggaatggcta
    10621 accgtcacgg gctgattact ggcgcaacgg ggacgggtaa aactgtcacg ttgcaaaaac
    10681 tggcagagtc attgtcggaa atcggtgttc cggtgtttat ggccgatgtg aaaggcgatc
    10741 tgaccggcgt cgagcaggca ggaacggcgt cggaaaaact gctcgcaagg cttaaaaata
    10801 tcggcgtcaa tgactggcaa ccgcatgcca atccggtggt ggtgtgggat atctttggcg
    10861 agaaaggcca tccggtgcgg gcgacggttt cggatctggg gccgctgttg ctggcacgac
    10921 tgttgaatct caacgatgtg caatctggcg tgctgaatat cattttccgc attgctgacg
    10981 atcagggatt gttgctgctc gactttaaag atctgcgggc aattacccag tacatcggcg
    11041 ataacgccaa atccttccag aatcagtacg gaaatatcag tagcgcatcg gttggtgcca
    11101 tccagcgcgg attactgtcg ctggaacagc aaggcgcagc acacttcttt ggtgagccga
    11161 tgctggatat caaagactgg atgcgcaccg atgccaacgg taaaggcgtt atcaatatcc
    11221 tcagcgccga gaaactttat cagatgccga aactgtacgc cgccagcctg ctgtggatgc
    11281 tttcagaatt gtatgaacaa ttgcctgaag cgggcgatct ggagaaacca aaactggtgt
    11341 ttttcttcga cgaggcacat ctgctgttta acgatgcacc gcaggtactg ctggataaga
    11401 ttgagcaggt gataaggctt attcgctcaa aaggcgtggg cgtctggttc gtttcgcaaa
    11461 acccgtctga tattccggat aatgtgctcg ggcagctagg taatcgcgtt cagcacgctt
    11521 tgcgggcttt tacgccaaaa gatcagaaag cagtgaaggc agcggcgcaa accatgcggg
    11581 ccaatccggc atttgatacc gaaaaggcaa tccaggaact ggggaccggc gaggcgttaa
    11641 tctcgtttct cgatgcaaaa ggaagtcctt ctgtggtgga acgggcgatg gtgatcgcac
    11701 cttgttcgcg aatggggccg gtgacggaag atgagcgtaa tggcctgatt aatcactctc
    11761 cggtgtatgg caaatatgaa gatgaggtgg accgcgagtc cgcctatgag atgctgcaaa
    11821 aaggctttca ggccagtacc gagcagcaaa ataatccccc cgtgaaaggt aaagaggtgg
    11881 cggtggatga cggtattctt ggtggattga aggatatttt gtttggcact accggaccac
    11941 gcggcgggaa gaaagatggt gtggtgcaaa caatggcgaa aagcgccgct cgccaggtga
    12001 cgaatcagat tgtacgcggg atgttgggga gtttgctggg ggggagaaaa aggtaagtca
    12061 gacgtgatct gtgcccctca ccctaaccct ctccccaaag gggcgagggg accgttcgaa
    12121 ctcgtatttg actttgtcaa caataataaa gggagctttc gctcccttgt tagattacga
    12181 ttttctcatt aacagccaca ggctgattaa gaagaagctg gcgcttggca acagtgcgcc
    12241 gatgatcggc gggatgccat aaaccaacgt cagcgggccg aagatctggt ccagtacgta
    12301 gaagacaaaa ccgaagctga taccggtgac cacacgcacg cccatcggta cgctacgcag
    12361 tgggccaaag ataaacgaca gcgccatcag catcatcacc gccacg
//
LOCUS       DAMZFT010000135        12345 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.135, whole genome shotgun sequence.
ACCESSION   DAMZFT010000135 DAMZFT010000000
VERSION     DAMZFT010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12345)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12345)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12345
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.135"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            248..631
                     /gene="secE"
                     /locus_tag="PN977_004033"
     CDS             248..631
                     /gene="secE"
                     /locus_tag="PN977_004033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007665874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HDI6044011.1"
                     /translation="MSANTEAQGSGRGLEAMKWVVVVALLLVAIVGNYLYRDIMLPLR
                     ALAVVILIAAAGGVALLTTKGKATVAFAREARTEVRKVIWPTRQETLHTTLIVAAVTA
                     VMSLILWGLDGILVRLVSFITGLRF"
     gene            633..1178
                     /gene="nusG"
                     /locus_tag="PN977_004034"
     CDS             633..1178
                     /gene="nusG"
                     /locus_tag="PN977_004034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005280494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HDI6044012.1"
                     /translation="MSEAPKKRWYVVQAFSGFEGRVATSLREHIKLHNMEDLFGEVMV
                     PTEEVVEIRGGQRRKSERKFFPGYVLVQMVMNDASWHLVRSVPRVMGFIGGTSDRPAP
                     ISDKEVDAIMNRLQQVGDKPRPKTLFEPGEMVRVNDGPFADFNGVVEEVDYEKSRLKV
                     SVSIFGRATPVELDFSQVEKA"
     gene            1337..1765
                     /gene="rplK"
                     /locus_tag="PN977_004035"
     CDS             1337..1765
                     /gene="rplK"
                     /locus_tag="PN977_004035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006121495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HDI6044013.1"
                     /translation="MAKKVQAYVKLQVAAGMANPSPPVGPALGQQGVNIMEFCKAFNA
                     KTDSIEKGLPIPVVITVYADRSFTFVTKTPPAAVLLKKAAGIKSGSGKPNKDKVGKIS
                     RAQLQEIAQTKAADMTGADIEAMTRSIEGTARSMGLVVED"
     gene            1769..2473
                     /gene="rplA"
                     /locus_tag="PN977_004036"
     CDS             1769..2473
                     /gene="rplA"
                     /locus_tag="PN977_004036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HDI6044014.1"
                     /translation="MAKLTKRMRVIREKVDATKQYDINEAIALLKELATAKFVESVDV
                     AVNLGIDARKSDQNVRGATVLPHGTGRSVRVAVFTQGANAEAAKAAGAELVGMEDLAD
                     QIKKGEMNFDVVIASPDAMRVVGQLGQVLGPRGLMPNPKVGTVTPNVAEAVKNAKAGQ
                     VRYRNDKNGIIHTTIGKVDFDADKLKENLEALLVALKKAKPTQAKGVYIKKVSISTTM
                     GAGVAVDQAGLSASVN"
     gene            2765..3262
                     /gene="rplJ"
                     /locus_tag="PN977_004037"
     CDS             2765..3262
                     /gene="rplJ"
                     /locus_tag="PN977_004037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002438625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HDI6044015.1"
                     /translation="MALNLQDKQAIVAEVSEVAKGALSAVVADSRGVTVDKMTELRKA
                     GREAGVYMRVVRNTLLRRAVEGTPFECLKDAFVGPTLIAYSMEHPGAAARLFKEFAKA
                     NAKFEVKAAAFEGELIPASQIDRLATLPTYEEAIARLMATMKEASAGKLVRTLAAVRD
                     AKEAA"
     gene            3329..3694
                     /gene="rplL"
                     /locus_tag="PN977_004038"
     CDS             3329..3694
                     /gene="rplL"
                     /locus_tag="PN977_004038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HDI6044016.1"
                     /translation="MSITKDQIIEAVAAMSVMDVVELISAMEEKFGVSAAAAVAVAAG
                     PVEAAEEKTEFDVILKAAGANKVAVIKAVRGATGLGLKEAKDLVESAPAALKEGVSKD
                     DAEALKKALEEAGAEVEVK"
     gene            4014..8042
                     /gene="rpoB"
                     /locus_tag="PN977_004039"
     CDS             4014..8042
                     /gene="rpoB"
                     /locus_tag="PN977_004039"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004870488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HDI6044017.1"
                     /translation="MVYSYTEKKRIRKDFGKRPQVLDVPYLLSIQLDSFQKFIEQDPE
                     GQYGLEAAFRSVFPIQSYSGNSELQYVSYRLGEPVFDVQECQIRGVTYSAPLRVKLRL
                     VIYEREAPEGTVKDIKEQEVYMGEIPLMTDNGTFVINGTERVIVSQLHRSPGVFFDSD
                     KGKTHSSGKVLYNARIIPYRGSWLDFEFDPKDNLFVRIDRRRKLPATIILRALNYTTE
                     QILDLFFEKVIFEIRDNKLQMELVPERLRGETASFDIEANGKVYVEKGRRITARHIRQ
                     LEKDDVKLIEVPVEYIAGKVVAKDYIDESTGELICAANMELSLDLLAKLSQSGHKRIE
                     TLFTNDLDHGPYISETLRVDPTNDRLSALVEIYRMMRPGEPPTREAAESLFENLFFSE
                     DRYDLSAVGRMKFNRSLLREEIEGSGILSKDDIIDVMKKLIDIRNGKGEVDDIDHLGN
                     RRIRSVGEMAENQFRVGLVRVERAVKERLSLGDLDTLMPQDMINAKPISAAVKEFFGS
                     SQLSQFMDQNNPLSEITHKRRISALGPGGLTRERAGFEVRDVHPTHYGRVCPIETPEG
                     PNIGLINSLSVYAQTNEYGFLETPYRKVTDGVVTDEIHYLSAIEEGNYVIAQANSNLD
                     EEGHFVEDLVTCRSKGESSLFSRDQVDYMDVSTQQVVSVGASLIPFLEHDDANRALMG
                     ANMQRQAVPTLRADKPLVGTGMERAVAVDSGVTAVAKRGGVVQYVDASRIVIKVNEDE
                     MYPGEAGIDIYNLTKYTRSNQNTCINQMPCVSLGEPVERGDVLADGPSTDLGELALGQ
                     NMRVAFMPWNGYNFEDSILVSERVVQEDRFTTIHIQELACVSRDTKLGPEEITADIPN
                     VGEAALSKLDESGIVYIGAEVTGGDILVGKVTPKGETQLTPEEKLLRAIFGEKASDVK
                     DSSLRVPNGVSGTVIDVQVFTRDGVEKDKRALEIEEMQLKQAKKDLSEELQILEAGLF
                     SRIRAVLVAGGVEAEKLDKLPRDRWLELGLTDEEKQNQLEQLAEQYDELKHEFEKKLE
                     AKRRKITQGDDLAPGVLKIVKVYLAVKRRIQPGDKMAGRHGNKGVISKINPIEDMPYD
                     ENGTPVDIVLNPLGVPSRMNIGQILETHLGMAAKGIGDKINAMLKQQQEVAKLREFIQ
                     RAYDLGADVRQKVDLSTFSDEEVMRLAENLRKGMPIATPVFDGAKEAEIKELLKLGDL
                     PTSGQIRLYDGRTGEQFERPVTVGYMYMLKLNHLVDDKMHARSTGSYSLVTQQPLGGK
                     AQFGGQRFGEMEVWALEAYGAAYTLQEMLTVKSDDVNGRTKMYKNIVDGNHQMEPGMP
                     ESFNVLLKEIRSLGINIELEDE"
     gene            8119..12342
                     /gene="rpoC"
                     /locus_tag="PN977_004040"
     CDS             8119..12342
                     /gene="rpoC"
                     /locus_tag="PN977_004040"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HDI6044018.1"
                     /translation="MKDLLKFLKAQTKTEEFDAIKIALASPDMIRSWSFGEVKKPETI
                     NYRTFKPERDGLFCARIFGPVKDYECLCGKYKRLKHRGVICEKCGVEVTQTKVRRERM
                     GHIELASPTAHIWFLKSLPSRIGLLLDMPLRDIERVLYFESYVVIEGGMTNLERQQIL
                     TEEQYLDALEEFGDEFDAKMGAEAIQALLKSMDLEQECEQLREELNETNSETKRKKLT
                     KRIKLLEAFVQSGNKPEWMILTVLPVLPPDLRPLVPLDGGRFATSDLNDLYRRVINRN
                     NRLKRLLDLAAPDIIVRNEKRMLQEAVDALLDNGRRGRAITGSNKRPLKSLADMIKGK
                     QGRFRQNLLGKRVDYSGRSVITVGPYLRLHQCGLPKKMALELFKPFIYGKLELRGLAT
                     TIKAAKKMVEREEAVVWDILDEVIREHPVLLNRAPTLHRLGIQAFEPVLIEGKAIQLH
                     PLVCAAYNADFDGDQMAVHVPLTLEAQLEARALMMSTNNILSPANGEPIIVPSQDVVL
                     GLYYMTRDCVNAKGEGMVLTGPKEAERLYRSGLASLHARVKVRITEYEKDANGELVAK
                     TSLKDTTVGRAILWMIVPKGLPYTIVNQALGKKAISKMLNTCYRILGLKPTVIFADQI
                     MYTGFAYAARSGASVGIDDMVIPEKKHEIISEAEAEVAEIQEQFQSGLVTAGERYNKV
                     IDIWAAANDRVSKAMMDNLQTETVINRDGQEEKQVSFNSIYMMADSGARGSAAQIRQL
                     AGMRGLMAKPDGSIIETPITANFREGLNVLQYFISTHGARKGLADTALKTANSGYLTR
                     RLVDVAQDLVVTEDDCGTHEGIMMTPVIEGGDVKEPLRDRVLGRVTAEDVLKPGTADI
                     LVPRNTLLHEQWCDLLEENSVDAVKVRSVVSCDTDFGVCAHCYGRDLARGHLINKGEA
                     IGVIAAQSIGEPGTQLTMRTFHIGGAASRAAAESSIQVKNKGSIKLSNVKSVVNSSGK
                     LVITSRNTELKLIDEFGRTKESYKVPYGAVLAKGDGEQVAGGETVANWDPHTMPVITE
                     VSGFVRFTDMIDGQTITRQTDELTGLSSLVVLDSAERTAGGKDLRPALKIVDAQGNDV
                     LIPGTDMPAQYFLPGKAIVQLEDGVQISSGDTLARIPQESGGTKDITGGLPRVADLFE
                     ARRPKEPAILAEISGIVSFGKETKGKRRLVITPVDGSDPYEEMIPKWRQLNVFEGERV
                     ERGDVISDGPEAPHDILRLRGVHAVTRYIVNEVQDVYRLQGVKINDKHIEVIVRQMLR
                     KATIVNAGSSDFLEGEQVEYSRVKIANRELEANGKVGATYSRDLLGITKASLATESFI
                     SAASFQETTRVLTEAAVAGKRDELRGLKENVIVGRLIPAGTGYAYHQDRMRRRAAGEA
                     PAAPQVTAEDASASLAELLNAGLGGSDNE"
BASE COUNT         3055 a         3069 c         3375 g         2846 t
ORIGIN      
        1 gcaaaagttc tgagctaatt gccgataaca tttgacgcaa tgcgcactaa aagggcatca
       61 tttgatgccc tttttgcacg ctttcgtacc agaacctggc tcatcagtga ttttctttgt
      121 cataatcatt gctgagacag gctctgttga gggcgtataa tccgaaaagc taatacgcgt
      181 ttcgatttgg tttgcctcgc gatcgcgggg tgaaaatgtt tgtagaaaac ttctgacagg
      241 ttggtttatg agtgcgaata ccgaagctca aggaagcggg cgcggcctgg aagcgatgaa
      301 gtgggtcgtt gtggtggcat tgctcctggt ggcgattgtc ggcaactatc tttatcgcga
      361 cattatgctg ccgctgcgtg cgctggccgt agtaattctg attgctgcag cgggtggtgt
      421 cgcgctgtta acgacaaaag gtaaagctac cgttgctttt gcccgtgaag cgcgtaccga
      481 agtccgtaag gtcatttggc cgactcgcca ggaaacattg cacaccacgc tgattgtggc
      541 tgcggttacc gcagtaatgt cactgatcct gtggggactg gatggtattc tggttcgcct
      601 ggtatccttt atcactggcc tgaggttctg agatgtctga agctcctaaa aagcgctggt
      661 acgtcgttca ggcgttttcc ggttttgaag gccgcgtagc aacgtcgctg cgtgagcata
      721 tcaaattaca caacatggaa gatttgtttg gtgaagtcat ggtaccaacc gaagaagtgg
      781 ttgaaatccg tggcggtcag cgtcgcaaaa gcgaacgtaa attcttccct ggctacgtcc
      841 tcgttcagat ggtgatgaac gacgcgagct ggcacctggt gcgcagcgta ccgcgtgtga
      901 tgggcttcat cggcggtact tccgatcgtc ctgcaccaat cagcgataaa gaagtcgatg
      961 cgattatgaa ccgcctgcag caggttggtg ataagccgcg tccgaaaacg ctgtttgaac
     1021 cgggtgaaat ggtccgtgtt aatgatggtc cgttcgctga cttcaacggt gttgttgaag
     1081 aagtggatta cgagaaatct cgtctgaaag tgtctgtttc tatcttcggt cgtgcgaccc
     1141 cggtagagct ggacttcagc caggttgaaa aagcctaacc cagcgatcaa aaaagcggcg
     1201 atttaatcgt tgcacaaggc gcgagattgg aatacaattt cgcgcctttt gtttttatgg
     1261 gccttgcccg taaaacgatt ttttatatca cggggagcct ctcagaggcg ttattaccca
     1321 acttgaggaa tttataatgg ctaagaaagt acaagcctat gtcaagctgc aggttgcagc
     1381 tggtatggct aacccgagtc cgccagtagg tccggctctg ggtcagcagg gcgtaaacat
     1441 catggaattc tgcaaagcgt tcaacgcaaa aactgattcc atcgaaaaag gtctgccgat
     1501 tccggtagta atcaccgttt acgctgaccg ttctttcact ttcgttacca agactccgcc
     1561 agcagcagtt ctgctgaaaa aagcggctgg tatcaagtct ggttccggta agccgaacaa
     1621 agacaaagtg ggtaaaattt cccgcgctca gctgcaggaa atcgcgcaga ccaaagctgc
     1681 cgacatgact ggtgccgaca ttgaagcgat gactcgctcc atcgaaggta ctgcacgttc
     1741 catgggcctg gtagtggagg actaagaaat ggctaaactg accaagcgca tgcgtgttat
     1801 ccgcgagaaa gttgatgcaa ccaaacagta cgacatcaac gaagctatcg ctctgctgaa
     1861 agaactggcg actgctaaat tcgtagaaag cgtggacgta gctgttaacc tcggcatcga
     1921 cgctcgtaaa tctgaccaga acgtacgtgg tgcaactgta ctgccgcacg gtactggccg
     1981 ttccgttcgc gtagccgtat ttacccaggg tgcaaacgct gaagctgcta aagctgcagg
     2041 tgctgaactg gtaggtatgg aagatctggc tgaccagatc aagaaaggcg aaatgaactt
     2101 tgacgttgtt attgcttctc cggatgcaat gcgcgttgtt ggccagctgg gccaggttct
     2161 gggtccgcgc ggcctgatgc caaacccgaa agtgggtact gtaacgccga acgttgctga
     2221 agcggttaaa aacgctaaag ctggccaggt tcgttaccgt aacgacaaaa acggcatcat
     2281 ccacaccacc atcggtaaag tggactttga cgctgacaaa ctgaaagaaa acctggaagc
     2341 tctgctggtt gcgctgaaaa aagcaaaacc gactcaggcg aaaggcgtgt acatcaagaa
     2401 agttagcatc tccaccacca tgggtgcagg tgttgcagtt gaccaggctg gcctgagcgc
     2461 ttctgtaaac taatgccttt acgtgggcgg tgattttgtc tacaatctta cccccacgta
     2521 tgctaacgaa agttagcagc taaaaagatt tgttcgttgg agcctggcct atccaggcct
     2581 ccgtcgaaga ccgcaggagt ttcgcaagaa acttaatccc ctgcgtagac ggtgacagaa
     2641 cgctaagatt attcttttat attctggctt gtttctgctc accgtaatta agacgctctc
     2701 tccgtttgga ggagtgaagt gagttccaga gattttctct ggcaaacatc caggagcaaa
     2761 gctaatggct ttaaatcttc aagacaaaca agcgattgtt gctgaagtca gcgaagtagc
     2821 caaaggcgcg ctgtctgcag tagttgcgga ttcccgtggc gtaactgtag ataaaatgac
     2881 tgaactgcgt aaagcaggtc gcgaagctgg cgtatacatg cgtgttgttc gtaacaccct
     2941 gctgcgccgt gctgttgaag gtactccgtt cgagtgcctg aaagacgcgt ttgttggtcc
     3001 gaccctgatt gcatactcta tggaacaccc gggcgctgct gctcgtctgt tcaaagagtt
     3061 cgcgaaagcg aatgcaaaat ttgaggtcaa agccgctgcc tttgaaggtg agctgatccc
     3121 ggcgtctcag atcgaccgcc tggcaactct gccgacctac gaagaagcaa ttgcacgcct
     3181 gatggcaacc atgaaagaag cttcggctgg caaactggtt cgtactctgg ctgctgtacg
     3241 cgatgcgaaa gaagctgctt aatcgcagtt atctttttaa cgcatccgct tacgtataaa
     3301 cttattctga tattcaggaa caatttaaat gtctatcact aaagatcaaa tcattgaagc
     3361 agttgcagct atgtctgtaa tggacgttgt agaactgatc tctgcaatgg aagaaaaatt
     3421 cggtgtttcc gctgctgctg ctgtagctgt agctgctggc ccggttgaag ctgctgaaga
     3481 aaaaactgaa ttcgacgtaa ttctgaaagc tgctggcgct aacaaagttg ctgttatcaa
     3541 agcagtacgt ggcgcaactg gcctgggtct gaaagaagct aaagacctgg tagaatctgc
     3601 accggctgct ctgaaagaag gcgtgagcaa agacgacgca gaagcactga aaaaagctct
     3661 ggaagaagct ggcgctgaag ttgaagttaa ataagccaac ccttccggtt gcagcctgag
     3721 aaatcaggct gatggctggt gactttttag tcaccagcct ttttgcgctg taaggcgcca
     3781 gtagcgtttc acactgtttg actactgctg tgcctttcaa tgcttgtttc tatcgacgac
     3841 ttaatatact gcgacaggac gtccgttctg tgtaaatcgc aatgaaatgg tttaagcgtg
     3901 atagcaacag gcattgcgga aagtgttcca ttttccggtc aacaaaatag tgttgcacaa
     3961 actgtccgct caatggacag atgggtcgac ttgtcagcga gctgaggaac cctatggttt
     4021 actcctatac cgagaaaaaa cgtattcgta aggattttgg taaacgtcca caagttctgg
     4081 atgtacctta tctcctttct atccagcttg actcgtttca gaaatttatc gagcaagatc
     4141 ctgaagggca gtatggtctg gaagctgctt tccgttccgt attcccgatt cagagctaca
     4201 gcggtaattc cgagctgcaa tacgtcagct accgccttgg cgaaccggtg tttgacgtcc
     4261 aggaatgtca aatccgtggc gtgacctatt ccgcaccgct gcgcgttaaa ctgcgtctgg
     4321 tgatctatga gcgcgaagcg ccggaaggca ccgtaaaaga cattaaagaa caagaagtct
     4381 acatgggcga aattccgctc atgacagaca acggtacctt tgttatcaac ggtactgagc
     4441 gtgttatcgt ttcccagctg caccgtagtc cgggcgtctt ctttgactcc gacaaaggta
     4501 aaacccactc ttcgggtaaa gtgctgtata acgcgcgcat catcccttac cgtggttcct
     4561 ggctggactt cgaattcgat ccgaaggaca acctgttcgt acgtatcgac cgtcgccgta
     4621 aactgcctgc gaccatcatt ctgcgcgccc tgaactatac cacagagcag atcctcgacc
     4681 tgttctttga aaaagttatc tttgaaatcc gtgataacaa gctgcagatg gaactggtgc
     4741 cggaacgcct gcgtggtgaa accgcatctt ttgacatcga agctaacggt aaagtgtacg
     4801 tagaaaaagg ccgccgtatt actgcgcgcc atattcgcca gctggaaaaa gacgacgtca
     4861 aactgatcga agtcccggtt gagtacatcg caggtaaagt ggttgctaaa gactatattg
     4921 atgagtctac cggcgagctg atctgcgcag cgaacatgga gctgagcctg gatctgctgg
     4981 ctaagctgag ccagtctggt cacaagcgta tcgaaacgct gttcaccaac gacctggatc
     5041 acggcccata tatctctgaa accttacgtg tcgacccaac taacgaccgt ctgagcgcac
     5101 tggtagaaat ctaccgtatg atgcgccctg gcgagccgcc gactcgtgaa gctgctgaaa
     5161 gcctgttcga gaacctgttc ttctccgaag accgctatga cttgtctgcg gttggtcgta
     5221 tgaagttcaa ccgttctctg ctgcgcgaag aaatcgaagg ttccggtatc ctgagcaaag
     5281 acgacatcat tgatgttatg aaaaagctca tcgatatccg taacggtaaa ggcgaagtcg
     5341 atgatatcga ccacctcggc aaccgtcgta tccgttctgt tggcgaaatg gcggaaaacc
     5401 agttccgcgt tggcctggtg cgtgtagagc gtgcggtgaa agagcgtctg tctctgggcg
     5461 atctggatac cctgatgcct caggatatga tcaacgccaa gccgatttct gcagcagtga
     5521 aagagttctt cggttccagc cagctgtctc agtttatgga ccagaacaac ccgctgtctg
     5581 agattacgca caaacgtcgt atctccgcac tcggcccggg tggtctgacc cgtgaacgtg
     5641 caggcttcga agttcgagac gtacacccga ctcactacgg tcgcgtatgt ccaatcgaaa
     5701 cccctgaagg tccgaacatc ggtctgatca actctctgtc cgtgtacgca cagactaacg
     5761 aatacggctt ccttgagact ccgtatcgta aagtgaccga cggtgttgta actgacgaaa
     5821 ttcactacct gtctgctatc gaagaaggca actacgttat cgcccaggcg aactccaacc
     5881 tggatgaaga aggccacttc gtagaagacc tggtaacttg ccgtagcaaa ggcgaatcca
     5941 gcttgttcag ccgtgaccag gttgactaca tggacgtatc cacccagcag gtggtatccg
     6001 tcggtgcgtc cctgatcccg ttcctggaac acgatgacgc caaccgtgca ttgatgggtg
     6061 cgaacatgca acgtcaggcc gttccgactc tgcgcgctga taagccgctg gttggtacgg
     6121 gtatggaacg tgctgttgcc gttgactccg gtgtaactgc ggttgctaaa cgtggtggtg
     6181 tcgttcagta cgtggatgct tcccgtatcg ttatcaaagt taacgaagac gagatgtatc
     6241 cgggtgaagc aggtatcgac atctacaacc tgaccaaata cacccgttct aaccagaaca
     6301 cttgtatcaa ccagatgccg tgtgtgtctc tgggggaacc ggtagagcgt ggcgacgtgc
     6361 tggcagatgg tccgtccacc gacctcggtg aactggcgct tggtcagaac atgcgcgtag
     6421 cgttcatgcc gtggaatggt tacaacttcg aagactccat cctcgtatcc gagcgtgttg
     6481 ttcaggaaga ccgtttcacc accatccaca ttcaggaact ggcgtgtgtg tcccgtgaca
     6541 ccaagctggg gccggaagag atcaccgctg acatcccgaa cgtgggtgaa gctgcgctct
     6601 ccaaactgga tgaatccggt atcgtttaca ttggtgcgga agtgaccggt ggcgacattc
     6661 tggttggtaa ggttacgccg aaaggcgaaa ctcagctgac cccagaagaa aaactgctgc
     6721 gtgcgatctt cggtgagaaa gcgtctgacg ttaaagactc ttctctgcgc gtaccaaacg
     6781 gtgtatccgg tacggttatc gacgttcagg tctttactcg cgatggcgta gaaaaagaca
     6841 aacgtgcgct ggaaatcgaa gaaatgcagc tcaaacaggc gaagaaagac ctgtctgaag
     6901 aactgcagat cctcgaagcg ggtctgttca gccgtatccg tgctgtgctg gtagccggtg
     6961 gcgttgaagc tgagaagctc gacaaactgc cgcgcgatcg ctggctggag ctgggcctga
     7021 ccgacgaaga gaaacaaaat cagctggaac agctggctga gcagtatgac gaactgaaac
     7081 acgagttcga gaagaaactc gaagcgaaac gccgcaaaat cacccagggc gacgatctgg
     7141 caccgggcgt gctgaagatt gttaaagtat atctggcggt taaacgccgt atccagcctg
     7201 gtgacaagat ggcaggtcgt cacggtaaca agggtgtaat ttctaagatc aacccgatcg
     7261 aagatatgcc ttacgatgaa aacggtacgc cggtagacat cgtactgaac ccgctgggcg
     7321 taccgtctcg tatgaacatc ggtcagatcc tcgaaaccca cctgggtatg gctgcgaaag
     7381 gtatcggcga caagatcaac gccatgctga aacagcagca agaagtcgcg aaactgcgcg
     7441 aattcatcca gcgtgcgtac gatctgggcg ctgacgttcg tcagaaagtt gacctgagta
     7501 ccttcagcga tgaagaagtt atgcgtctgg ctgaaaacct gcgcaaaggt atgccaatcg
     7561 caacgccggt cttcgacggt gcgaaagaag cagaaattaa agagctgctg aaacttggcg
     7621 acctgccgac ttctggtcag atccgcctgt acgacggccg cactggtgaa cagttcgagc
     7681 gtccggtaac cgttggttac atgtacatgc tgaaactgaa ccacctggtc gacgacaaga
     7741 tgcacgcgcg ttccaccggt tcttacagcc tggttactca gcagccgctg ggtggtaagg
     7801 cacagttcgg tggtcagcgt ttcggggaga tggaagtgtg ggcgctggaa gcatacggcg
     7861 cagcatacac cctgcaggaa atgctcaccg ttaagtctga tgacgtgaac ggtcgtacta
     7921 agatgtataa aaacatcgtg gacggcaacc atcagatgga gccgggcatg ccagaatcct
     7981 tcaacgtatt gttgaaagag attcgttcgc tgggtatcaa catcgaactg gaagacgagt
     8041 aattctcgct caaacaggtc actgctgtcg gggtaaaacc cggcagcgga ttgtgctaac
     8101 tccgacggga gcaaatccgt gaaagattta ttaaagtttc tgaaagcgca gactaaaacc
     8161 gaagagtttg atgcgatcaa aattgctctg gcttcgccag acatgatccg ttcatggtct
     8221 ttcggtgaag ttaaaaagcc ggaaaccatc aactaccgta cgttcaaacc agaacgtgac
     8281 ggccttttct gcgcccgtat ctttgggccg gtaaaagatt acgagtgcct gtgcggtaag
     8341 tacaagcgcc tgaaacaccg tggcgtcatc tgtgagaagt gcggtgttga agtgacccag
     8401 actaaagtac gccgtgagcg tatgggccac atcgaactgg cttccccgac tgcgcacatc
     8461 tggttcctga aatcgctgcc gtcccgtatc ggcctgctgc tcgatatgcc gctgcgcgat
     8521 atcgaacgcg tactgtactt cgaatcctat gtggttatcg aaggcggtat gaccaacctg
     8581 gagcgtcagc agatcctgac tgaagagcag tatctggacg cgctggaaga gttcggtgac
     8641 gaattcgacg cgaagatggg tgcggaagca atccaggccc tgctgaagag catggatctg
     8701 gagcaagagt gcgaacagct gcgtgaagag ctgaacgaaa ccaactccga aaccaagcgt
     8761 aaaaagctga ccaagcgtat caaactgctg gaagcgttcg ttcagtctgg taacaaacca
     8821 gagtggatga tcctgaccgt tctgccggta ctgccgccag atctgcgtcc gctggttccg
     8881 ctggatggtg gtcgtttcgc gacttctgac ctgaacgatc tgtatcgtcg cgtcattaac
     8941 cgtaacaacc gtctgaaacg tctgctggat ctggctgcgc cggacatcat cgtacgtaac
     9001 gaaaaacgta tgctgcagga agcggtagac gccctgctgg ataacggtcg tcgcggtcgt
     9061 gcaatcaccg gttctaacaa gcgtcctctg aaatctttgg ccgacatgat caaaggtaaa
     9121 cagggtcgtt tccgtcagaa cctgctcggt aagcgtgttg actactccgg tcgttctgta
     9181 atcaccgtag gtccatacct gcgtctgcat cagtgcggtc tgccgaagaa aatggcactg
     9241 gagctgttca aaccgttcat ctacggcaag ctggaactgc gtggtcttgc taccaccatt
     9301 aaagctgcga agaaaatggt tgagcgcgaa gaagctgtcg tttgggatat cctggacgaa
     9361 gttatccgcg aacacccggt actgctgaac cgtgcaccga ctctgcaccg tctgggtatc
     9421 caggcatttg aaccggtact gatcgaaggt aaagctatcc agctgcaccc gctggtttgt
     9481 gcggcatata acgccgactt cgatggtgac cagatggctg ttcacgtacc gctgacgctg
     9541 gaagcccagc tggaagcgcg tgcgctgatg atgtctacca acaacatcct gtccccggca
     9601 aacggcgaac caatcatcgt tccgtctcag gacgttgtac tgggtctgta ctacatgacc
     9661 cgcgactgtg ttaacgccaa aggcgaaggc atggtgctga ctggcccgaa agaagcagaa
     9721 cgtctgtatc gctctggtct ggcttctctg catgcgcgcg ttaaagtgcg tatcaccgag
     9781 tatgaaaaag atgctaacgg tgaattagtg gcgaaaacca gcctgaaaga cacgactgtt
     9841 ggccgtgcca ttctgtggat gattgtaccg aaaggtctgc cttacaccat cgtcaaccag
     9901 gcgctgggta aaaaagcaat ctccaaaatg ctgaacacct gctaccgcat tctcggtctg
     9961 aaaccgaccg ttatttttgc ggaccagatc atgtataccg gctttgccta tgcagcgcgt
    10021 tctggtgcat ctgttggtat cgatgacatg gtcatcccgg agaagaaaca cgaaatcatc
    10081 tccgaggcag aagcagaagt tgctgaaatt caggagcagt tccagtctgg tctggtaact
    10141 gcgggcgaac gctacaacaa agttatcgat atctgggctg cggcgaacga tcgtgtatcc
    10201 aaagcgatga tggataacct gcaaactgaa accgttatta accgtgacgg tcaggaagag
    10261 aagcaggttt ccttcaacag catctacatg atggccgact ccggtgcgcg tggttctgcg
    10321 gcacagattc gtcagctggc gggtatgcgt ggtctgatgg cgaagccgga tggctccatc
    10381 atcgaaacgc caatcaccgc gaacttccgt gaaggtctga acgtactcca gtacttcatc
    10441 tccacccacg gtgctcgtaa aggtctggcg gataccgcac tgaaaacggc gaactccggt
    10501 tacctgactc gtcgtctggt tgacgtggcg caggacctgg tggttaccga agacgattgt
    10561 ggtacccatg aaggtatcat gatgactccg gttatcgagg gtggtgacgt taaagagccg
    10621 ctgcgcgatc gcgtattggg tcgtgtaact gctgaagacg ttctgaagcc gggtactgct
    10681 gatatcctcg ttccgcgcaa cacgctgctg cacgaacagt ggtgtgacct gctggaagag
    10741 aactctgtcg acgcggttaa agtacgttct gttgtatctt gtgacaccga ctttggtgta
    10801 tgtgcgcact gctacggtcg tgacctggcg cgtggccacc tcatcaacaa aggtgaagca
    10861 atcggtgtaa tcgcggcaca gtccatcggt gaaccgggta cacagctgac catgcgtacg
    10921 ttccacatcg gtggtgcggc atctcgtgcg gctgctgaat ccagcatcca ggtgaaaaac
    10981 aaaggtagca tcaagctcag caacgtgaag tcggttgtga actccagcgg taaactggtt
    11041 atcacttccc gtaacaccga actgaaactg atcgacgaat tcggtcgtac caaagaaagc
    11101 tacaaagtac cttacggtgc ggtactggcg aaaggcgatg gcgaacaggt tgctggcggc
    11161 gaaaccgttg caaactggga cccgcacacc atgccggtta tcaccgaagt aagcggtttt
    11221 gtacgcttta ctgacatgat cgacggccag accattactc gtcagaccga cgaattgacc
    11281 ggtctgtctt cgctggtggt tctggattcc gcagaacgta ccgcaggtgg taaagatctg
    11341 cgtccggcac tgaaaatcgt tgatgctcag ggtaacgacg ttctgatccc gggtaccgat
    11401 atgcctgcgc agtacttcct gccgggtaaa gcgattgttc agctggaaga tggcgtacag
    11461 atcagctctg gtgacaccct ggcgcgtatt ccgcaggaat ccggcggtac caaggacatc
    11521 accggtggtc tgccgcgcgt tgcggacctg ttcgaagcac gtcgtccgaa agagccggca
    11581 atcctggctg aaatcagcgg tatcgtttcc ttcggtaaag aaaccaaagg taaacgtcgt
    11641 ctggttatca ccccggtaga cggtagcgat ccgtacgaag agatgattcc gaaatggcgt
    11701 cagctcaacg tgttcgaagg tgaacgtgta gaacgtggtg acgtaatttc cgacggtccg
    11761 gaagcgccgc acgacattct gcgtctgcgt ggtgttcatg ctgttactcg ttacatcgtt
    11821 aacgaagtac aggacgtata ccgtctgcag ggcgttaaga ttaacgataa acacatcgaa
    11881 gttatcgttc gtcagatgct gcgtaaagct accatcgtta acgcgggcag ctccgacttc
    11941 ctggaaggtg aacaggttga atactctcgc gtcaagatcg caaaccgcga actggaagcg
    12001 aacggcaaag tgggggcaac ttactcccgc gatctgctgg gtatcaccaa agcgtctctg
    12061 gcaaccgagt ccttcatctc cgcggcatcg ttccaggaga ccactcgcgt gctgaccgaa
    12121 gcagccgttg cgggcaaacg cgacgaattg cgcggcctga aagagaacgt tatcgtgggt
    12181 cgtctgatcc cggcaggtac cggttacgcg taccaccagg atcgtatgcg tcgccgtgct
    12241 gcgggtgaag ctccggctgc accgcaggtg actgcagaag acgcatctgc cagcctggca
    12301 gaactgctga acgcaggtct gggcggttct gataacgagt aatcg
//
LOCUS       DAMZFT010000136        12261 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.136, whole genome shotgun sequence.
ACCESSION   DAMZFT010000136 DAMZFT010000000
VERSION     DAMZFT010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12261)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12261)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12261
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.136"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..484
                     /locus_tag="PN977_004041"
     CDS             <1..484
                     /locus_tag="PN977_004041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HemK family protein methyltransferase"
                     /protein_id="HDI6044019.1"
                     /translation="TGTGAIALALASERPDCEITAVDCMPDAVSLAQRNAQNLAIKNI
                     HILQSDWFSALAGQQFAMIVSNPPYIDEQDPHLQQGDVRFEPLTALVAADSGMADIVH
                     IIEQSRNALVSGGFLLLEHGWQQGEAVRQAFIHAGYHDVETCRDYGDNERVTLGRYYQ
                     "
     gene            481..873
                     /locus_tag="PN977_004042"
     CDS             481..873
                     /locus_tag="PN977_004042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SirB family protein"
                     /protein_id="HDI6044020.1"
                     /translation="MTSFSTLLSVHLISIALSVGLLTLRFWLRYQKHPQAFARWTRIV
                     PPVVDTLLLLSGIALMAKAHILPFSGQAQWLTEKLFGVIIYIVLGFIALDYRRMHSQQ
                     ARIIAFPLALVVLYIIIKLATTKVPLLG"
     gene            877..1686
                     /locus_tag="PN977_004043"
     CDS             877..1686
                     /locus_tag="PN977_004043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat-containing protein"
                     /protein_id="HDI6044021.1"
                     /translation="MRSLADFEFNKAPLCEGMILACEAIRRDFPSQDVYDELERLVSL
                     AKEEISQLLPLEEQLEKLIALFYGDWGFKASRGVYRLSDALWLDQVLKNRQGSAVSLG
                     AVLLWVANRLDLPLLPVIFPTQLILRIECPDGEIWLINPFNGESLSEHMLDVWLKGNI
                     SPSAELFYEDLDEADNIEVIRKLLDTLKASLMEENQMELALRTSEALLQFNPEDPYEI
                     RDRGLIYAQLDCEHVALNDLSYFVEQCPEDPISEMIRAQINNIAHKHIVLH"
     gene            1722..2576
                     /gene="kdsA"
                     /locus_tag="PN977_004044"
     CDS             1722..2576
                     /gene="kdsA"
                     /locus_tag="PN977_004044"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HDI6044022.1"
                     /translation="MKQKVVSIGDINVANDLPFVLFGGMNVLESRDLAMRICEHYVTV
                     TQKLGIPYVFKASFDKANRSSIHSYRGPGLEEGMKIFQELKQTFGVKIITDVHEPSQA
                     QPVADVVDVIQLPAFLARQTDLVEAMAKTGAVINVKKPQFVSPGQMGNIVDKFKEGGN
                     EKVILCDRGANFGYDNLVVDMLGFSIMKKVSGNSPVIFDVTHALQCRDPFGAASGGRR
                     AQVAELARAGMAVGLAGLFIEAHPDPEHAKCDGPSALPLAKLEPFLKQMKAIDDLVKG
                     FEELDTSK"
     gene            complement(2725..2832)
                     /locus_tag="PN977_004045"
     CDS             complement(2725..2832)
                     /locus_tag="PN977_004045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HDI6044023.1"
                     /translation="MTLTQFAMTFWHDLAAPILAGIITAAIVSWWRNRK"
     gene            complement(3260..3367)
                     /locus_tag="PN977_004046"
     CDS             complement(3260..3367)
                     /locus_tag="PN977_004046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HDI6044024.1"
                     /translation="MTLAQFAMIFWHDLAAPILAGIITAVIVSWWRNRK"
     gene            complement(3773..4873)
                     /gene="chaA"
                     /locus_tag="PN977_004047"
     CDS             complement(3773..4873)
                     /gene="chaA"
                     /locus_tag="PN977_004047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-potassium/proton antiporter ChaA"
                     /protein_id="HDI6044025.1"
                     /translation="MSNAQEAVKTRHKETSLIFPVLALVVLFLWGSSQTLPVVIGINL
                     LALIGILSSAFSVVRHADVLAHRLGEPYGSLILSLSVVILEVSLISALMATGDAAPTL
                     MRDTLYSIIMIVTGGLVGFSLLLGGRKFATQYMNLFGIKQYLIALFPLAIIVLVFPMA
                     LPAANFSTGQALLVALISAAMYGVFLLIQTKTHQSLFVYEHEDDSDDDDPHHGKPSAH
                     SSLWHAIWLIIHLIAVIAVTKMNASSLETLLDSMNAPVAFTGFLVALLILSPEGLGAL
                     KAVLNNQVQRAMNLFFGSVLATISLTVPVVTLIAFMTGNELQFALGAPEMVVMVASLV
                     LCHISFSTGRTNVLNGAAHLALFAAYLMTIFA"
     gene            5143..5373
                     /gene="chaB"
                     /locus_tag="PN977_004048"
     CDS             5143..5373
                     /gene="chaB"
                     /locus_tag="PN977_004048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative cation transport regulator ChaB"
                     /protein_id="HDI6044026.1"
                     /translation="MPYKTKSDLPESVKHVLPSHAQDIYKEAFNSAWDQYKDKEDRRD
                     DASREETAHKVAWAAVKHEYAKGDDDKWHKKS"
     gene            5531..6226
                     /gene="chaC"
                     /locus_tag="PN977_004049"
     CDS             5531..6226
                     /gene="chaC"
                     /locus_tag="PN977_004049"
                     /EC_number="4.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-specific
                     gamma-glutamylcyclotransferase"
                     /protein_id="HDI6044027.1"
                     /translation="MITRDFLMNADCKTAFGAIEESLLWSAEQRAASLAATLACRPDE
                     GPVWIFGYGSLMWNPALEFTESCTGTLVGWHRAFCLRLTAGRGTAHQPGRMLALKEGG
                     RTTGVAYRLPEETLEQELTLLWKREMITGCYLPTWCQLDLDDGRTVNAIVFIMDPRHP
                     EYESDTRAQVIAPLIAAASGPLGTNAQYLFSLEQELIKLGMQDDGLNDLLVSVKKLLA
                     ENYPDAVLRPGFA"
     gene            complement(6270..6623)
                     /gene="ychN"
                     /locus_tag="PN977_004050"
     CDS             complement(6270..6623)
                     /gene="ychN"
                     /locus_tag="PN977_004050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/F sulfur relay family protein YchN"
                     /protein_id="HDI6044028.1"
                     /translation="MQKIVIVANGAPYGSESLFNSLRLAIALREQESNLDLRLFLMSD
                     AVTAGLRGQKPGEGYNIQQMLEILTAQNVPVKLCKTCTDGRGISTLPLIDGVEIGTLV
                     ELAQWTLSADKVLTF"
     gene            6808..8202
                     /gene="ychO"
                     /locus_tag="PN977_004051"
     CDS             6808..8202
                     /gene="ychO"
                     /locus_tag="PN977_004051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter invasin YchO"
                     /protein_id="HDI6044029.1"
                     /translation="MSRFVPRIIPFYLLLLAAGGTANAQSTFEQKAANPFDNNNDGLP
                     DLGMAPENHDGEKHFAEIVKDFGETSMNDNGLDTGEQAKAFALGKVRDALSQQVNQHV
                     ESWLSPWGNASVDVKVDNEGHFTGSRGSWFVPLQDNDRYLTWSQLGLTQQDDGLVSNV
                     GVGQRWARGNWLVGYNTFYDNLLDENLQRAGFGAEAWGEYLRLSANFYQPFAAWHEQT
                     ATQEQRMARGYDLTARMRMPFYQHLNTSVSVEQYFGDRVDLFNSGTGYHNPVALSLGL
                     NYTPVPLVTVTARHKQGESGENQNNLGLNLNYRFGVPLKKQLSAGEVAESQSLRGSRY
                     DNPQRNNLPTLEYRQRKTLTVFLATPPWDLKPGETVPLKLQIRSRYGIRQLIWQGDTQ
                     ILSLTPGAQANSEEGWTLIMPDWQNGEGASNHWRLSVVVEDNQGQRVSSNEITLTLVE
                     PFDALSNDELRWEP"
     gene            complement(8203..8853)
                     /gene="narL"
                     /locus_tag="PN977_004052"
     CDS             complement(8203..8853)
                     /gene="narL"
                     /locus_tag="PN977_004052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator NarL"
                     /protein_id="HDI6044030.1"
                     /translation="MSNQEPATILLIDDHPMLRTGVKQLISMAPDITVVGEASNGEQG
                     IELAESLDPDLILLDLNMPGMNGLETLDKLREKSLSGRIVVFSVSNHEEDVVTALKRG
                     ADGYLLKDMEPEDLLKALHQAAAGEMVLSEALTPVLAASLRANRATTERDVNQLTPRE
                     RDILKLIAQGLPNKMIARRLDITESTVKVHVKHMLKKMKLKSRVEAAVWVHQERIF"
     gene            complement(8846..10642)
                     /gene="narX"
                     /locus_tag="PN977_004053"
     CDS             complement(8846..10642)
                     /gene="narX"
                     /locus_tag="PN977_004053"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarX"
                     /protein_id="HDI6044031.1"
                     /translation="MLKRCLSPLTLVNQVALIVLLSTAIGLAGMAVSGWLVQGVQGSA
                     HAINKAGSLRMQSYRLLAAVPLSEKDKPLIKEMEQTAFSAELTRAAERDGQLAQLQGL
                     QDYWRNELIPALMRAQNRETVSADVSQFVAGLDQLVSGFDRTTEMRIETVVLVHRVMA
                     VFMALLLVFTIIWLRARLLQPWRQLLAMASAVSHRDFTQRANISGRNEMAMLGTALNN
                     MSAELAESYAVLEQRVQEKTAGLEHKNQILSFLWQANRRLHSRAPLCERLSPVLNGLQ
                     NLTLLRDIELRVYDTDDEENHQEFTCQPDMTCDDKGCQLCPRGILPVGDRGTTLKWRL
                     ADSHTQYGILLATLPQGRHLSHDQQQLVDTLVEQLTATLALDRHQERQQQLIVMEERA
                     TIARELHDSIAQSLSCMKMQVSCLQMQGDALPESSRELLSQIRNELNASWAQLRELLT
                     TFRLQLTEPGLRPALEASCEEYSAKFGFPVKLDYQLPPRLVPSHQAIHLLQIAREALS
                     NALKHSQASEVVVTVAQNDNQVKLTVQDNGCGVPENAIRSNHYGMIIMRDRAQSLRGD
                     CRVRRRESGGTEVVVTFIPEKTFTDVQGDTHE"
     gene            10981..>12261
                     /gene="narK"
                     /locus_tag="PN977_004054"
     CDS             10981..>12261
                     /gene="narK"
                     /locus_tag="PN977_004054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate transporter NarK"
                     /protein_id="HDI6044032.1"
                     /translation="MSHSSAPERATGAVITDWRPEDPAFWQQRGQRIASRNLWISVPC
                     LLLAFCVWMLFSAVAVNLPKVGFNFTTDQLFMLTALPSVSGALLRVPYSFMVPIFGGR
                     RWTAFSTGILIIPCVWLGFAVQDTSTPYSVFIIISLLCGFAGANFASSMANISFFFPK
                     QKQGGALGLNGGLGNMGVSVMQLVAPLVVSLSIFAVFGSQGVKQPDGTELYLANASWV
                     WVPFLAIFTIAAWFGMNDLATSKASIKEQLPVLKRGHLWIMSLLYLATFGSFIGFSAG
                     FAMLSKTQFPDVQILQYAFFGPFIGALARSAGGALSDRLGGTRVTLVNFILMAIFSGL
                     LFLTLPTDGQGGSFMAFFAVFLALFLTAGLGSGSTFQMISVIFRKLTMDRVKAEGGSD
                     ERAMREAATDTAAALGFISAIGAIGGFFIPKAFGS"
BASE COUNT         2966 a         2996 c         3175 g         3124 t
ORIGIN      
        1 gacgggtacc ggggcgattg cgctggcgct ggctagcgag cgcccggact gcgaaattac
       61 cgctgtagat tgtatgcctg atgctgtctc tctggcgcaa cgtaatgccc agaatctggc
      121 gatcaaaaat atccacattc tgcaaagtga ctggtttagc gcgctagccg ggcagcagtt
      181 tgcgatgatt gtcagcaatc cgccgtatat tgacgagcag gacccacatc ttcaacaagg
      241 cgatgtccgc tttgagccgc tcactgcact ggttgcggct gacagtggaa tggccgacat
      301 cgtgcatatc atcgaacagt cgcgtaacgc tctggtatcc ggcggctttc tgcttctgga
      361 acatggctgg cagcagggcg aagcggtgcg acaggcattt atccacgcgg gatatcatga
      421 cgtcgaaacc tgtcgggact atggtgataa cgagcgcgta acgctcggcc gctattatca
      481 atgacaagtt tttctacact gcttagcgtt catctcatta gcattgcgct ttctgtaggg
      541 ctattaacct tacgtttctg gctacgttat cagaaacatc cacaggcttt tgctcgttgg
      601 acgcgcattg tgccgccggt tgtcgatacg ctgttattgt taagcggcat tgcgttgatg
      661 gctaaagcgc acatcctgcc attttccggg caggcacagt ggctgactga aaagctgttt
      721 ggagttatca tttatatcgt tttgggtttt attgcactcg attatcgtcg tatgcacagt
      781 cagcaggcgc gcattattgc cttcccgctg gcgttggtgg tgctgtacat catcattaaa
      841 ctcgccacca caaaagtacc gttactgggg taagtcatga gatcgttagc tgatttcgaa
      901 tttaataaag cgccattgtg cgaaggcatg atcctggctt gcgaagcaat ccgccgcgat
      961 tttccctcgc aagatgttta cgacgaactg gagcgtctcg ttagtctggc gaaggaagaa
     1021 atcagccagc ttctgccttt agaagaacag ctggaaaaac tgatcgcgct gttttacggc
     1081 gactggggat ttaaagcctc acgcggcgtt tatcgtcttt ccgatgcatt atggctggac
     1141 caggtgttaa agaatcgaca gggcagtgcg gtatcattag gtgccgtttt attatgggtc
     1201 gcgaatcgtc tcgatttgcc gctgctgccg gtgattttcc ctacgcagct gatattgcgc
     1261 attgaatgtc cggatggcga aatttggctg attaatcctt ttaacggtga atcgttaagt
     1321 gaacatatgc tggatgtgtg gttaaaggga aatatcagcc cgtcggcgga actgttttat
     1381 gaagaccttg atgaagctga taacattgag gtaatccgca aattgctgga tacactcaaa
     1441 gcctcgttga tggaagaaaa tcagatggag ctggcgctac gtaccagcga agctttatta
     1501 caatttaacc cggaagatcc ctatgaaatt cgcgatcgcg ggttgattta tgcgcaactg
     1561 gattgcgaac acgttgcgtt gaacgattta agttatttcg ttgaacagtg tccggaagac
     1621 ccgatcagcg aaatgatccg tgcgcaaata aataacatcg cgcataaaca tattgtgctg
     1681 cattaattaa tcgacatttt actcaagatt aaggcgatcc tatgaaacaa aaagtggtta
     1741 gcattggcga catcaacgta gcaaatgacc tgccgttcgt actgtttggc ggtatgaacg
     1801 tgctggaatc tcgcgatctg gcgatgcgca tttgcgagca ctatgtaacc gtgacccaga
     1861 aactgggtat cccttacgta ttcaaagcct cttttgacaa agccaaccgc tcctccatcc
     1921 actcttatcg tggaccgggc ctggaagaag gaatgaaaat cttccaggag ttgaagcaga
     1981 cttttggcgt gaaaattatc accgacgttc acgaaccaag ccaggctcag cccgttgctg
     2041 atgttgtaga tgtgattcag ttgccggcgt ttcttgctcg ccagacagac ctggttgaag
     2101 ccatggcgaa aaccggtgcg gtaattaacg tcaagaaacc gcagtttgtc agcccgggac
     2161 agatgggtaa tatcgttgat aaattcaaag aaggcggcaa cgaaaaagtg attctttgcg
     2221 atcgcggtgc taacttcggt tacgacaacc tggtcgttga tatgctgggc ttcagcatca
     2281 tgaagaaagt gtctggtaat tcgccggtga ttttcgacgt tacccacgca ctgcaatgtc
     2341 gcgatccatt tggcgcagct tccggcggtc gtcgtgctca ggtggctgag ctggcaagag
     2401 ccggtatggc ggtaggtctg gcggggctgt ttattgaagc gcatccggat ccagaacatg
     2461 cgaaatgtga tgggccatcc gcgctgccgc tggctaaact ggaaccgttc ctcaagcaaa
     2521 tgaaagcgat tgatgatctg gtgaaaggtt tcgaagaact ggataccagc aagtaatctt
     2581 ttttgcttga aaaataaagt cttagcgttc tgcgttaaga cttttttcat gggtgccgga
     2641 tacaaaaaag gccgcaggct attacctctg cggccggttt cgggcgcata ttgccatcac
     2701 ggcagcctga cgcccgtttt caccttactt ccggttacgc caccagctga caatcgctgc
     2761 ggtaataatt cccgccagga tcggtgctgc caggtcgtgc cagaaagtca tggcaaactg
     2821 cgtgagcgtc atatagccgc cttgttgtaa tgacaacatt ttgcggctat tcttgaattg
     2881 ttctggttca agattagccc ccgttctgtt gtcaggttgt acctctcaac gtgcgggggt
     2941 tttctctttc cagcaaccaa tgccaccagg gataaagccc ccgcaacatt gcgcctcacc
     3001 ggataactcc ggcttggtgt ggatactacg tctcaattca tcttcacttc atccctgaaa
     3061 tgtttgcaat gaagagtgca ttccggtttt tcaacagctg ttacagtcgt ttcatgagtg
     3121 ctctggatga tgcttccagc tcgggttgcc aatatttact tgcagaagag ataaagacaa
     3181 aaagggccgc aggctgttac ccctgcggcc ggtttcgggc gcatattgcc atcacggcag
     3241 cctgacgccc gttttcacct tacttccggt tacgccacca gctgacaatc actgcggtaa
     3301 taatccccgc caggatcggt gccgccaggt cgtgccagaa aatcatggca aactgcgcga
     3361 gcgtcatata gccgccttgt tgtaatgaca acattttgcg gctattcttg aattgttgat
     3421 ggttcaagat tagcccccgt tctgttgtca ggttttacct ctcaacgtgc gggggttttc
     3481 tctttccagc aaccaatgcc accagggata aagcccccgc aacattgcgc ctcaccggat
     3541 aacgccggct tggtgtggat attacgtctc aattcatctt cacttcatcc ctgaaatgtt
     3601 tgcaataaag agtacattcc ggcttttcaa cagctgttgc agtggtttcg tgcaggttgt
     3661 ggtacaggct cgcaattctg attaacgacc tgttcaggta gtatgaagaa atacaataag
     3721 caggaattta tattatctcc cccgatttct gtcatcagag gagatataac tgtcaggcaa
     3781 atatcgtcat caaataggcg gcaaacagtg ccagatgcgc tgcgccattg agcacgttag
     3841 ttcgtccggt ggagaaggag atatggcaca gtactaaaga ggccaccatc accaccattt
     3901 ctggcgcacc aagtgcaaac tgtaattcgt tacccgtcat aaaggcaatt aacgtgacga
     3961 caggtacggt aagcgaaatg gttgctaaca ctgaaccaaa gaacagattc atcgcgcgct
     4021 ggacctggtt attcaacact gcttttaacg cacctaaacc ttccggcgac agaatcaaca
     4081 gagccaccag gaagccagta aaggcgacag gcgcattcat gctgtcgagc aatgtctcca
     4141 gcgagctggc gttcattttg gtcaccgcaa taacggcaat cagatggata atcaaccaga
     4201 tagcatgcca caggctgcta tgggcagacg gtttaccgtg atgcgggtcg tcatcatcac
     4261 tgtcatcttc gtgctcgtag acaaacaaac tttgatgcgt tttggtctgg atcagcaaaa
     4321 atacgccata cattgccgca gaaattaatg ctaccagtaa cgcctgaccg gttgaaaaat
     4381 tcgccgcagg cagagccatt ggaaatacca gtacgattat cgccaggggg aacagcgcaa
     4441 ttaaatactg cttgatacca aacagattca tatattgggt ggcaaacttg cgtccgccca
     4501 acaataatga aaagccaacc agcccaccgg taacaatcat aatgattgaa tagagcgtat
     4561 cacgcattag cgttggcgcg gcgtcgccgg ttgccattaa agctgaaatc aaactgactt
     4621 caagaataac cactgaaagg ctaagaataa gcgaaccgta aggttctccc aggcgatggg
     4681 ctaatacgtc cgcatgacgg acaacactaa aggcgctact taaaatacca ataagagcaa
     4741 gaagattgat gccaatgacc actggtagtg tctggctgct tccccacagg aacagcacta
     4801 ccagcgccag aaccgggaaa ataagcgaag tctccttgtg gcgggttttt accgcctctt
     4861 gagcatttga cattatggtt atccctttgc agatgaattt atcgaaaatg taaaaaatag
     4921 gtaggaaaaa taacagaaag tgtctggata tcggtaacat tttacgaatt tttaccccgc
     4981 tgtcgatttt ttactcatcg ggccataaaa ataagcaatg cgtttagaca atgtttgttt
     5041 tagcactcct taagaagagt ctgacatgaa aattcttatg ttttggcaag agtagatatt
     5101 gttgaccaca cttaatgttc aactttgtaa aaggagtcaa cgatgccgta taaaacgaaa
     5161 agcgatctgc cggaaagcgt aaagcacgtt ctaccgtctc atgcccagga tatctataaa
     5221 gaagcgttca acagcgcatg ggatcaatat aaagataaag aagatcggcg tgatgacgcc
     5281 agtcgcgaag aaaccgcgca taaagtggcc tgggctgctg tgaagcatga atatgccaaa
     5341 ggggatgatg ataaatggca taaaaaatcg taaaaccggt agcttagtta aagctattcg
     5401 tgcggtgttg ccttgcaagt ggtccgtgga ttgcatattg tcccgttagt ggtttcaaaa
     5461 tgagcagtaa aaatgtccgg aagacaccaa aaagttgtcg cagggaagta tgcagtggcg
     5521 gaggtgtaag gtgataacgc gtgatttctt gatgaatgcc gattgtaaaa cggcatttgg
     5581 tgccattgaa gaatcactct tatggtcagc agagcaacgg gcggcttcgc tggcggcgac
     5641 gctggcttgt cgacctgatg agggaccggt gtggatcttc ggttatggat cgttgatgtg
     5701 gaatccggca ctggagttta ccgagtcgtg caccggtaca ctggttggat ggcatcgcgc
     5761 gttttgcctg cgcctgaccg ccgggcgcgg gactgcgcac cagccgggac ggatgcttgc
     5821 actgaaagag ggcggacgca ccacaggcgt cgcctatcga ctgccagaag agacgctgga
     5881 gcaggaacta accctgttgt ggaagcgcga gatgattacc ggctgttatc tgccaacctg
     5941 gtgtcagctt gatcttgatg atggacgcac agtaaacgcc attgtgttta ttatggaccc
     6001 gcgacatcca gaatatgaat ctgacactcg cgctcaggtc atcgcgccgt tgattgcggc
     6061 ggcgagcggt ccgctgggaa ccaacgcaca atacctgttt tcactggaac aggagcttat
     6121 caaactggga atgcaggacg atgggctgaa tgatttgctg gtatcggtaa aaaaactgct
     6181 ggcggagaat tatccggatg ctgtgttacg gccgggattc gcctgagtaa acttccctca
     6241 tagtggggcg tcagacgccc cctcaaacat taaaatgtga gcactttatc ggctgacagc
     6301 gtccattgcg ccagttcaac aagagtaccg atttccaccc catcaatcag aggaagtgtg
     6361 ctaatcccgc gtccgtcagt acaggttttg cacaatttca ccggtacatt ctgagcggta
     6421 aggatctcca gcatttgctg aatgttgtag ccttcccctg gtttttgccc gcgcaacccg
     6481 gctgtgaccg catcagacat caggaacaga cgcagatcca gattgctctc ctgctctcgt
     6541 aacgcaatgg ccagccgcaa gctgttaaac aaggattcgc tcccgtaagg tgctccattg
     6601 gcaacgatca cgattttttg cattatttac tcctgtattc agggaattag acactcatct
     6661 tctatcttac cgcttctgca gcgtctgacc aatcggtcac atttttaagg attttcctga
     6721 aagcgcgaga aaatacgaca aaaggtgcca gtaatcgtta ttctttaagg ctatggtttt
     6781 tcatttttac cggaagttac cgacgttttg agccgtttcg ttcctcgcat tattccgttt
     6841 tatttactct tgctggcggc aggcggtaca gctaacgcac aatctacctt cgagcaaaaa
     6901 gcggcaaatc cctttgataa taacaatgat ggtctgccgg atttaggcat ggcacctgaa
     6961 aatcatgatg gggaaaaaca ctttgcggaa attgtgaaag atttcggcga aaccagtatg
     7021 aatgataacg ggctggatac tggcgagcag gcaaaagctt tcgcattagg aaaagtccgc
     7081 gacgcgctta gtcaacaggt taatcagcac gtagagtcct ggctatcacc gtggggaaat
     7141 gccagtgttg acgtcaaagt ggataacgaa ggacatttca ccggcagtcg cggaagctgg
     7201 tttgtgccgt tacaagataa tgatcgttat ctcacctgga gccagcttgg tcttactcag
     7261 caggatgatg gcttggtgag caatgtgggc gtagggcaac gctgggcgcg cggcaactgg
     7321 ctggtgggtt ataacacttt ttatgacaac ttgctggacg aaaatcttca gcgagcgggc
     7381 tttggtgccg aagcgtgggg cgaatatttg cgactatcgg caaactttta tcagccgttt
     7441 gctgcatggc atgaacagac agccacgcag gaacagcgga tggcgcgcgg gtacgacctg
     7501 acagcccgga tgcgcatgcc gttctatcaa cacctcaata ccagtgtcag cgtagaacag
     7561 tattttggtg atcgtgtcga tttgtttaac tctggtacgg gttatcacaa tcccgtcgcg
     7621 ttgagtctgg gattaaatta cacccctgtg cccttagtca ctgtgacggc ccggcataaa
     7681 cagggtgaaa gtggcgagaa tcaaaataac ctcgggctga atcttaacta ccgctttggt
     7741 gtaccgctca aaaaacaact ttctgcgggc gaagttgccg aaagtcagtc gttacgtggt
     7801 agtcgctatg acaatccgca gcgaaataat cttccgactc ttgagtaccg acagcgaaaa
     7861 accttaacgg tgtttctggc gacaccgccg tgggatctaa aacctggcga aacagtgccg
     7921 ctgaaattac aaatccgcag tcgttacggt attcggcaac tgatttggca gggcgatacg
     7981 cagatattaa gtttgacgcc gggcgcacaa gccaacagtg aggagggctg gacgctgatc
     8041 atgcctgact ggcaaaacgg ggaaggcgca agcaatcact ggcgattgtc agtagtggtg
     8101 gaagataacc aggggcagcg tgtctcctcc aatgagatca cgctaacgct tgtcgaaccg
     8161 ttcgacgcat tgtcaaacga cgaactgcgc tgggaaccgt aatcagaaaa tgcgctcctg
     8221 atgcacccat accgcagctt ccacgcgaga cttgagcttc attttcttca gcatgtgctt
     8281 gacgtgcact tttactgtgc tttcggtgat atccaggcgg cgggcaatca tcttgttcgg
     8341 caaaccctgg gcaatcagct tgagaatatc gcgctcgcgt ggggttaact ggttaacatc
     8401 gcgttcagta gtggcacggt tagcgcgcaa gctggcggcc agaacaggcg ttaatgcttc
     8461 gcttaatacc atttcgccag cagctgcctg atgcaatgct ttcagcagat cttccggttc
     8521 catatctttt aacagatagc catccgcgcc gcgtttcagt gcggtgacca catcttcttc
     8581 atggttagag acgctgaata ccacaatgcg accggagagg gacttttcgc gcagtttatc
     8641 cagcgtttcc agaccgttca tgccgggcat attgagatct aacaggatca gatcgggatc
     8701 aagagattcc gccagttcaa taccctgttc gccattactc gcttcgccaa ccacggtgat
     8761 atctggtgcc atactgataa gctgttttac gccagttcgc agcatcgggt gatcgtcgat
     8821 cagcaggata gtagccggtt cctgattact catgggtatc tccttggacg tctgtgaaag
     8881 ttttttcggg aataaaggtg acaaccactt cggtgccacc tgattcacga cggcggacgc
     8941 ggcaatcgcc tcgtaaactt tgcgcacggt cgcgcattat tatcatgccg tagtgattgc
     9001 tgcggatggc attttcaggc acgccgcagc cgttatcctg gacggtcagt ttgacctgat
     9061 tatcgttttg cgccaccgtc accacgacct cactcgcttg cgaatgtttg agggcgttac
     9121 ttaatgcctc acgggcaatt tgcaacaagt ggattgcctg atgcgaaggc accagacgag
     9181 gcggcaattg ataatccagc ttcaccggga agccaaattt ggcgctgtac tcttcgcaac
     9241 tcgcctccag cgccggacgt aatccaggct cggtgagctg caaacggaat gtggtgagca
     9301 attcacgcaa ctgcgcccag gatgcattca gttcgttacg gatctgactt aacagttcgc
     9361 ggctgctttc tggcagcgca tcgccctgca tctgtaaaca actcacctgc atcttcatgc
     9421 aagagagaga ttgggcaata gaatcatgca gttcgcgcgc aatggtggca cgctcttcca
     9481 tcacgatcaa ctgttgctga cgttcctgat ggcgatccag cgccagcgtg gcggtgagtt
     9541 gttcaaccag ggtatccacc agttgttgtt gatcatggct aagatgacgc ccctgcggca
     9601 gggtcgccag caaaataccg tactgcgtat gagagtcagc cagccgccac ttcagggttg
     9661 tgccgcgatc gccaacgggt aatatgccgc gcgggcagag ctggcagcct ttatcatcac
     9721 aagtcatatc tggctggcag gtaaactcct gatgattctc ttcatcatca gtgtcataca
     9781 cccgcagttc gatatcacgt agcagggtta aattctgtaa gccgttgagt accggtgaca
     9841 ggcgttcaca cagcggggcg cgggaatgca aacggcggtt agcctgccat aaaaaagaga
     9901 ggatctgatt tttatgctcc agcccggcag ttttctcctg aacccgctgc tcaagtacgg
     9961 cataactttc ggccagttct gcagacatat tgttcaatgc agttccaagc atcgccattt
    10021 cgttgcgccc gctgatgttt gcgcgttggg taaaatcgcg atggctgacg gcactcgcca
    10081 ttgccagcag ttgccgccac ggttgtagca gtcgcgcccg caaccagata atagtgaaca
    10141 ccagtaaaag tgccataaat accgccatta cccgatggac cagtaccact gtctcaatgc
    10201 gcatttccgt ggtgcggtca aaaccagata ccagctgatc aagcccggca acaaactggc
    10261 tgacatccgc tgacaccgtt tcgcggtttt gtgcacgcat cagcgcaggg atcagttcat
    10321 tacgccagta atcttgtaaa ccctgtaatt gcgccagttg tccgtctcgt tccgctgctc
    10381 gagtcaactc ggcgctaaat gccgtttgtt ccatttcttt aattaagggc ttgtctttct
    10441 cgcttaatgg cactgccgcc aacagacggt aactttgcat gcgcagcgat cccgctttgt
    10501 tgatcgcatg ggcgctgccc tgaacgcctt gcaccagcca gccagaaact gccatccctg
    10561 ccagtccaat agcagtagaa agcaacacaa taagcgcaac ctgattaacc agggtgagcg
    10621 gagagagaca acgtttaagc atgtaaacct cttccttcgg gctttaaatg agcaataacc
    10681 ttaatgaatg tgacgataca ttctggaatg gcagtattct cggctattgg ctgaagtata
    10741 cccatacccg gaaagagtta ctccttattt gccgcgtggt tagtcgcttt acatcggtaa
    10801 gggtagggat tttacagcac cgtgaaaaat ctcataattt ttatgaaatc actgtactca
    10861 ctatgggtaa tgataaatat caatgataga taaagttatc ttatcgtttg atttacatca
    10921 aattgccttt agctacagac actaaggtgg cagacatcga aacgagtatc agaggtgtct
    10981 atgagtcact catccgcccc cgaaagggct actggagctg tcattacaga ttggcgaccg
    11041 gaagatcctg cgttctggca acaacgcggt caacgtattg ccagccgcaa cctgtggatt
    11101 tccgttccct gtctgctgct ggcgttttgc gtatggatgt tgttcagcgc tgttgcggtg
    11161 aacttaccga aagtcggttt taattttacg accgatcagc tatttatgtt gactgcgctg
    11221 ccttcggttt ctggcgcgtt attacgtgtt ccatactcct ttatggttcc tatcttcggt
    11281 ggtcgtcgct ggacggcgtt cagcaccggt attctgatta ttccttgcgt ctggctgggt
    11341 tttgccgtgc aggatacctc cacgccttat agcgtcttca tcatcatctc tctgctgtgc
    11401 ggctttgctg gcgcgaactt cgcatccagt atggcaaaca tcagcttctt ctttccgaaa
    11461 cagaagcagg gtggcgcgct gggtctgaat ggtggtctgg gcaacatggg cgtcagcgtc
    11521 atgcagttgg ttgctccgct ggtggtatca ctgtcgattt tcgcagtatt tggtagccag
    11581 ggtgtcaaac agccggatgg gactgagctg tatctggcga atgcgtcctg ggtatgggtg
    11641 ccgttccttg ccatcttcac cattgcggcg tggtttggca tgaacgatct tgctacctcg
    11701 aaagcctcca tcaaggagca gttgccggta ctcaaacggg gtcatctgtg gattatgagc
    11761 ctgctgtatc tggcaacctt cggctccttc atcggcttct ccgcgggctt tgcgatgctg
    11821 tcaaaaacgc agttcccgga tgttcagatt ctgcaatacg ctttcttcgg gccgtttatt
    11881 ggtgcgctgg cgcgttctgc aggtggtgca ttatctgacc gtctgggcgg aactcgtgtc
    11941 acgctggtga actttatcct gatggcgatt ttcagcggcc tgctgttcct gaccttaccg
    12001 actgacggac agggcggaag cttcatggcg ttcttcgcag tcttcctggc gctgttcctg
    12061 acagctgggc tgggtagtgg ttccactttc cagatgattt ccgtgatctt ccgtaaactg
    12121 acaatggatc gcgtgaaagc agaagggggt tctgacgaac gtgcgatgcg tgaagcggca
    12181 accgacacgg cggcggcgct gggtttcatc tctgcgattg gcgcgattgg tggcttcttt
    12241 atcccgaaag cgtttggtag c
//
LOCUS       DAMZFT010000137        12229 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.137, whole genome shotgun sequence.
ACCESSION   DAMZFT010000137 DAMZFT010000000
VERSION     DAMZFT010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12229)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12229)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12229
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.137"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..983
                     /gene="dgt"
                     /locus_tag="PN977_004055"
     CDS             <1..983
                     /gene="dgt"
                     /locus_tag="PN977_004055"
                     /EC_number="3.1.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="dGTPase"
                     /protein_id="HDI6044033.1"
                     /translation="LCHFEGNAQGIRLVHTLMRMNLTWAQVGGILKYTRPAWWRGETP
                     ETHHYLMKKPGYYLSEEAYIARLRKELNLALYSRFPLTWIMEAADDISYCVADLEDAV
                     EKRIFTVEQLYHHLHEAWGQHEKGSLFSLVVENAWEKSRSNSLSRSTEDQFFMYLRVN
                     TLNKLVPYAAQRFIDNLPAIFAGTFNHALLEDASECSDLLKLYKNVAVKHVFSHPDVE
                     QLELQGYRVISGLLEIYRPLLSLSLSDFTELVEKERVKRFPIESRLFHKLSTRHRLAY
                     VEAVSKLPSDSPEFPLWEYYYRCRLLQDYISGMTDLYAWDEYRRLMAVEQ"
     gene            1113..2537
                     /gene="degP"
                     /locus_tag="PN977_004056"
     CDS             1113..2537
                     /gene="degP"
                     /locus_tag="PN977_004056"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegP"
                     /protein_id="HDI6044034.1"
                     /translation="MKKTTLALSALALSLGLALSPLSATAAETSSATTAQQMPSLAPM
                     LEKVMPSVVSINVEGSTTVNTPRMPRNFQQFFGDDSPFCQEGSPFQSSPFCQGGQGGN
                     GGGQQQKFMALGSGVIIDADKGYVVTNNHVVDNATVIKVQLSDGRKFDAKMVGKDPRS
                     DIALIQIQNPKNLTAIKMADSDALRVGDYTVAIGNPFGLGETVTSGIVSALGRSGLNA
                     ENYENFIQTDAAINRGNSGGALVNLNGELIGINTAILAPDGGNIGIGFAIPSNMVKNL
                     TSQMVEYGQVKRGELGIMGTELNSDLAKAMKVDAQRGAFVSQVLPNSSAAKAGIKAGD
                     VITSLNGKPISSFAALRAQVGTMPVGSKLTLGLLRDGKQVNVNLELQQSSQNQVDSST
                     IFNGIEGAEMSNKGKDQGVVVNNVKTGTPAAQIGLKKGDVIIGANQQAVKNIAELRKV
                     LDSKPSVLALNIQRGDSTIYLLMQ"
     gene            2692..3849
                     /gene="cdaR"
                     /locus_tag="PN977_004057"
     CDS             2692..3849
                     /gene="cdaR"
                     /locus_tag="PN977_004057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CdaR"
                     /protein_id="HDI6044035.1"
                     /translation="MAGWHLDTKMAQDIVARTMRIIDTNINVMDARGRIIGSGDRERI
                     GELHEGALLVLSQGRVVDIDDAVARHLHGVRQGINLPLRLEGEIVGVIGLTGEPENLR
                     KYGELVCMTAEMMLEQSRLMHLLAQDSRLREELVMNLIQAEENTPALTEWAQRLGIDL
                     NQPRVVAIVEVDSGQLGVDSAMAELQQLQNALTTPERNNLVAIVSLTEMVVLKPALNS
                     FGRWDAEDHRKRVEQLITRMKEYGQLRFRVSLGNYFTGPGGIARSYRTAKTTMVVGKQ
                     RMPESRCYFYQDLMLPVLLDSLRGDWQANELARPLARLKAMDNNGLLRRTLAAWFRHN
                     VQPLATSKALFIHRNTLEYRLNRISELTGLDLGNFDDRLLLYVALQLDEER"
     gene            complement(3903..4289)
                     /locus_tag="PN977_004058"
     CDS             complement(3903..4289)
                     /locus_tag="PN977_004058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000272186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3461 family protein"
                     /protein_id="HDI6044036.1"
                     /translation="MYDNLKSLGITNPEEIDRYSLRQEANNDILKIYFQKDKGEFFAK
                     SVKFKYPRQRKTVVADGVGQGYKEVQEISPNLRYIIDELDQICQRDRSEVDLKRKILD
                     DLRHLESVVTNKISEIEADLEKLTRK"
     gene            complement(4604..5428)
                     /gene="dapD"
                     /locus_tag="PN977_004059"
     CDS             complement(4604..5428)
                     /gene="dapD"
                     /locus_tag="PN977_004059"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HDI6044037.1"
                     /translation="MQQLQNIIETAFERRAEITPANADTVTREAVNQVIALLDSGALR
                     VAEKIDGQWVTHQWLKKAVLLSFRINDNQVIEGAESRYFDKVPMKFANYDAARFQKEG
                     FRVVPPAAVRQGAFIARNTVLMPSYVNIGAYVDEGTMVDTWATVGSCAQIGKNVHLSG
                     GVGIGGVLEPLQANPTIIEDNCFIGARSEVVEGVIVEEGSVISMGVYIGQSTRIYDRE
                     TGEIHYGRVPAGSVVVSGNLPSKDGKYSLYCAVIVKKVDAKTRGKVGINELLRTID"
     gene            complement(5459..8131)
                     /gene="glnD"
                     /locus_tag="PN977_004060"
     CDS             complement(5459..8131)
                     /gene="glnD"
                     /locus_tag="PN977_004060"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     uridylyltransferase/uridylyl-removing protein GlnD"
                     /protein_id="HDI6044038.1"
                     /translation="MNTLPEQYANTALPTLPGQPQNPCGWPRDELTVCGIKAHIDTFQ
                     RWLGDAFDNGISAEELVEARTEFIDQLLQRLWIDAGFSQIADLALVAVGGYGRGELHP
                     LSDIDLLILSRKKLPDDQAQKVGELLTLLWDVKLEVGHSVRTLEECMLEGLSDLTVAT
                     NLIESRLLIGDVALFLELQKHIFSEGFWPSDKFYAAKVEEQNQRHQRYHGTSYNLEPD
                     IKSSPGGLRDIHTLQWVARRHFGATSLDEMVGFGFLTSAERAELNECLHILWRIRFAL
                     HLVVSRYDNRLLFDRQLSVAQRLNYSGEGNEPVERMMKDYFRVTRRVSELNQMLLQLF
                     DEAILALPADEKPRPIDDEFQLRGTLIDLRDETLFMRQPEAILRMFYTMVRNSAITGI
                     YSTTLRQLRHARRHLQQPLCNIPEARKLFLSILRHPGAVRRGLLPMHRHSVLGAYMPQ
                     WSHIVGQMQFDLFHAYTVDEHTIRVMLKLESFASEETRQRHPLCVDVWPRLPSTELIF
                     IAALFHDIAKGRGGDHSILGAQDVVHFAELHGLNSRETQLVAWLVRQHLLMSVTAQRR
                     DIQDPEVIKQFAEEVQTENRLRYLVCLTVADICATNETLWNSWKQSLLRELYFATEKQ
                     LRRGMQNTPDMRERVRHHQLQALALLRMDNIDEEALHQIWSRCRANYFVRHSPNQLAW
                     HARHLLQHDLSKPLVLLSPQATRGGTEIFIWSPDRPYLFAAVCAELDRRNLSVHDAQI
                     FTTRDGMAMDTFIVLEPDGSPLSADRHEVIRFGLEQVLTQSSWQPPQPRRQPAKLRHF
                     TVETEVTFLPTHTDRKSFLELIALDQPGLLARVGKIFADLGISLHGARITTIGERVED
                     LFIIATADRRALNNELQQEVHQRLTEALNPNDKG"
     gene            complement(8193..8987)
                     /gene="map"
                     /locus_tag="PN977_004061"
     CDS             complement(8193..8987)
                     /gene="map"
                     /locus_tag="PN977_004061"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HDI6044039.1"
                     /translation="MAISIKTPEDIEKMRVAGRLAAEVLEMIEPYVKPGVSTGELDRI
                     CNDYIVNEQHAVSACLGYHGYPKSVCISINEVVCHGIPDDAKLLKDGDIVNIDVTVIK
                     DGFHGDTSKMFIVGKPTIMGERLCRITQESLYLALRMVKPGINLREIGAAIQKFVEAE
                     GFSVVREYCGHGIGRGFHEEPQVLHYDSRETNVVLKPGMTFTIEPMVNAGKKEIRTMK
                     DGWTVKTKDRSLSAQYEHTIVVTDNGCEILTLRKDDTIPAIISHDE"
     gene            9355..10080
                     /gene="rpsB"
                     /locus_tag="PN977_004062"
     CDS             9355..10080
                     /gene="rpsB"
                     /locus_tag="PN977_004062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HDI6044040.1"
                     /translation="MATVSMRDMLKAGVHFGHQTRYWNPKMKPFIFGARNKVHIINLE
                     KTVPMFNEALAELNKIASRKGKILFVGTKRAASEAVKDAALSCDQFFVNHRWLGGMLT
                     NWKTVRQSIKRLKDLETQSQDGTFDKLTKKEALMRTRELEKLENSLGGIKDMGGLPDA
                     LFVIDADHEHIAIKEANNLGIPVFAIVDTNSDPDGVDFVIPGNDDAIRAVTLYLGAVA
                     ATVREGRSQDLASQAEESFVEAE"
     gene            10215..11066
                     /gene="tsf"
                     /locus_tag="PN977_004063"
     CDS             10215..11066
                     /gene="tsf"
                     /locus_tag="PN977_004063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HDI6044041.1"
                     /translation="MAEITASLVKELRERTGAGMMDCKKALTEANGDIELAIENMRKS
                     GAIKAAKKAGNVAADGVIKTKIDGNYGIILEVNCQTDFVAKDAGFQAFADKVLDAAVA
                     GKITDVEVLKAQFEEERVALVAKIGENINIRRVAALEGDVLGSYQHGARIGVLVAAKG
                     ADEELVKHIAMHVAASKPEFIKPEDVSAEVVEKEYQVQLDIAMQSGKPKEIAEKMVEG
                     RMKKFTGEVSLTGQPFVMEPSKTVGQLLKEHNAEVTGFIRFEVGEGIEKVETDFAAEV
                     AAMSKQS"
     gene            11213..11938
                     /gene="pyrH"
                     /locus_tag="PN977_004064"
     CDS             11213..11938
                     /gene="pyrH"
                     /locus_tag="PN977_004064"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000224576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HDI6044042.1"
                     /translation="MATNAKPVYKRILLKLSGEALQGTEGFGIDASILDRMAQEIKEL
                     VELGIQVGVVIGGGNLFRGAGLAKAGMNRVVGDHMGMLATVMNGLAMRDALHRAYVNA
                     RLMSAIPLNGVCDSYSWAEAISLLRNNRVVILSAGTGNPFFTTDSAACLRGIEIEADV
                     VLKATKVDGVFTADPAKDPTATMYEQLTYSEVLEKELKVMDLAAFTLARDHKLPIRVF
                     NMNKPGALRRVVMGEKEGTLITE"
     gene            12088..>12229
                     /gene="frr"
                     /locus_tag="PN977_004065"
     CDS             12088..>12229
                     /gene="frr"
                     /locus_tag="PN977_004065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622421.1"
                     /note="Rrf; Frr; ribosome-recycling factor; release factor
                     4; RF4; recycles ribosomes upon translation termination
                     along with release factor RF-3 and elongation factor EF-G;
                     A GTPase-dependent process results in release of 50S from
                     70S; inhibited by release factor RF-1; essential for
                     viability; structurally similar to tRNAs; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-recycling factor"
                     /protein_id="HDI6044043.1"
                     /translation="MISDIRKDAEVRMDKCVEAFKTQISKIRTGRASPSLLDGIVVEY
                     YGT"
BASE COUNT         3037 a         2964 c         3233 g         2995 t
ORIGIN      
        1 acttatgtca ttttgaaggt aacgcacagg gtattcgttt ggtgcataca ttgatgcgga
       61 tgaatctcac ctgggcacag gttggcggta ttttaaaata tacccgtccg gcgtggtggc
      121 gtggcgaaac gcctgagaca catcactatt taatgaaaaa gccgggttat tatctttctg
      181 aggaagccta tattgcccgg ttgcgtaaag aacttaattt ggcgctttac agtcgttttc
      241 cattaacgtg gattatggaa gctgccgacg acatctccta ttgtgtggca gatcttgaag
      301 atgcggtaga gaaaagaata tttaccgttg agcagcttta tcatcatttg cacgaagcgt
      361 ggggccagca tgagaaaggt tcgctctttt cgctggtggt tgaaaatgcc tgggaaaaat
      421 cacgctcaaa tagtttaagc cgcagtacgg aagatcagtt ttttatgtat ttaagggtaa
      481 acactctaaa taaactggta ccctacgcgg cacaacgatt tattgataat ctgcctgcga
      541 ttttcgccgg aacgtttaat catgcattat tggaagatgc cagcgaatgc agcgatcttc
      601 ttaagcttta taaaaatgtc gctgtaaaac atgtgtttag ccatccagat gtcgagcagc
      661 ttgaattgca gggctatcgg gtcattagcg gattattaga gatttatcgc cctttattaa
      721 gcctgtcgtt atcagacttt actgaactgg tagaaaaaga acgggtgaaa cgtttcccta
      781 ttgaatcgcg cttattccac aaactctcga cgcgccatcg gctggcctat gtcgaggctg
      841 tcagtaaatt accgtcagat tctcctgagt ttccgctatg ggaatattat taccgttgcc
      901 gcctgctgca ggattatatc agcggtatga ccgacctcta tgcctgggat gaataccgac
      961 gtctgatggc cgtagaacaa taaccaggct tttgtaaaga cgaacaataa atttttacct
     1021 tttgcagaaa ctttagttcg gaacttcgcg ctataaaacg aatctgaaga acacagcaat
     1081 tttgcgttat ctgttaatcg agactgaaat acatgaaaaa aaccacatta gcactgagtg
     1141 cactggctct gagtttaggt ttggcgttat ctccgctctc tgcaacggcg gctgagactt
     1201 cttcagcaac gacagcccag cagatgccaa gccttgcacc gatgctcgaa aaggtgatgc
     1261 cttcagtggt cagcattaac gtagaaggta gcacaaccgt taatacgccg cgtatgccgc
     1321 gtaatttcca gcagttcttc ggtgatgatt ctccgttctg ccaggaaggt tctccgttcc
     1381 agagttctcc gttctgccag ggtggccagg gcggtaatgg tggcggccag caacagaaat
     1441 tcatggcgct gggttccggc gtcatcattg atgccgataa aggctatgtc gtcaccaaca
     1501 accacgttgt tgataacgcg acggtgatta aagtccaact gagcgatggc cgtaagttcg
     1561 acgcgaagat ggttggcaaa gatccgcgct ctgatatcgc gctgatccag atccagaacc
     1621 cgaaaaacct gaccgcaatt aagatggcgg attctgatgc actgcgcgtg ggtgattaca
     1681 ccgtagcgat tggtaacccg tttggtctgg gcgagacggt aacttccggg attgtctctg
     1741 cgctggggcg tagcggcctg aatgccgaaa actacgaaaa cttcatccag accgatgcag
     1801 cgattaaccg tggtaactcc ggtggtgcgc tggttaacct gaacggtgaa ctgatcggta
     1861 tcaacaccgc gatcctcgca ccggacggcg gcaacatcgg tatcggtttt gctatcccga
     1921 gcaacatggt gaaaaacctg acctcgcaga tggtggaata cggccaggtg aaacgcggtg
     1981 agttgggtat tatgggcact gagctgaact ccgatctggc gaaagcgatg aaagttgacg
     2041 cccagcgcgg tgctttcgta agccaggttc tgccgaattc ttccgccgcg aaagcgggca
     2101 ttaaagcggg tgatgtgatc acctcactga acggtaagcc aatcagcagc tttgccgcac
     2161 tgcgtgctca ggtgggcact atgccggtag gtagcaaact gaccctgggc ttactgcgcg
     2221 acgggaagca ggttaacgtg aacctggaac ttcagcagag cagccagaat caggttgatt
     2281 ccagcaccat cttcaacggc attgaaggcg ctgagatgag taacaaaggc aaagatcagg
     2341 gcgtggtggt gaacaacgtg aaaacgggca ctccggctgc gcagatcggc ctgaagaaag
     2401 gtgatgtgat tattggcgcg aaccagcagg cagtgaaaaa catcgctgaa ctgcgtaaag
     2461 ttctcgacag caaaccgtct gtgctggcac tgaacattca gcgcggcgac agcaccatct
     2521 acctgttaat gcagtaatct ccctcaaccc cttcctgaaa acgggaaggg gttctcctta
     2581 caatctgtga acttcaccac aactccatac atcttcatca tcctttgggc atttgcacaa
     2641 tgccgtacgt tacgtacttc cttatgctaa gccgtgcata atggaggact tatggctggc
     2701 tggcatcttg ataccaaaat ggcgcaggat atcgtggcac gtaccatgcg catcatcgat
     2761 accaatatca acgtaatgga tgcccgcggg cgaattatcg gcagcggcga tcgtgagcgt
     2821 attggtgaat tgcacgaagg agcattgctg gtactttcac agggacgagt cgtcgatatc
     2881 gatgacgcgg tggcacgtca tctgcacggt gtgcggcagg ggattaatct accgttacgg
     2941 ctggaaggtg aaattgtcgg cgtaattggc ctgacaggtg aaccagagaa tctgcgtaaa
     3001 tatggcgaac tggtctgcat gacggctgaa atgatgctgg aacagtcgcg gttgatgcac
     3061 ctgttggcgc aggatagccg tttgcgggaa gaactggtga tgaacctgat tcaggctgag
     3121 gagaatactc ccgcacttac ggaatgggcg caacggctgg ggatcgatct caatcaaccg
     3181 cgagtggtag ctattgttga ggtcgacagc ggtcagcttg gcgtggacag cgcaatggcg
     3241 gagttacagc aactgcaaaa cgcgctgact acgcccgagc gtaataatct ggtggcaatt
     3301 gtttcgctga ccgaaatggt ggtgttgaaa ccggcgttga actcttttgg gcgctgggat
     3361 gcagaagatc atcgtaagcg agttgaacaa ctgattaccc gcatgaaaga gtacggccag
     3421 ctgcgttttc gcgtttcgct gggcaactat tttaccggtc ctggcggtat tgcccgatcc
     3481 tatcgtacgg cgaaaacgac gatggtggtg ggtaaacagc ggatgccaga aagtcgctgc
     3541 tatttttatc aggatctgat gttgcctgtg ttactcgaca gtttgcgtgg cgactggcag
     3601 gccaacgaac tggcgcgacc gctggcgcgg ctgaaagcga tggacaataa tggtttactg
     3661 cggcgaacgt tggcggcgtg gtttcgtcac aatgtgcaac cgctggcaac gtcaaaggcg
     3721 ctgtttattc atcgtaatac tctggagtat cggcttaatc gtatatcgga actgaccggg
     3781 cttgatttgg gcaattttga tgacaggttg ctgctgtatg tggcgttaca actggatgaa
     3841 gagcggtagg tcgatgacaa aattacagcc gggtgagact ttacccggcc cacagaatga
     3901 cattatttac gcgtcagttt ttccagatcg gcttcaatct cgctgatctt attggttact
     3961 actgactcca ggtgacgtaa gtcgtcgagg atcttacgct taagatcaac ttcgctgcgg
     4021 tcacgctggc agatttgatc aagctcatca atgatatacc gtagattcgg gctgatttcc
     4081 tggacttctt tataaccctg acccacacca tcagcgacga ccgttttacg ctgacgcgga
     4141 tatttaaact tcacgctctt ggcgaaaaac tcgcctttgt ctttctggaa atagattttc
     4201 agaatatcgt tgttggcttc ctgccggagg ctgtaacgat caatttcttc aggattggta
     4261 atacccagac ttttcagatt gtcgtacata gcgttacctc aaaatgagtc agtaaattgt
     4321 gcttatttta gcatttggcc tggcccgccc ggctcgactg tgatcgcaga aagccttttt
     4381 cttccagact tctatcatgg cgtaaaaatc aaaaaattac ctgctttatt gtggcgatat
     4441 tccatgggat ggattagcac atttatcttc gttttgcgaa ttacatgaca gtccgaaaca
     4501 agatgaaaaa gtatttaaaa aaggggggag gaagaatgag caggacgaat tattctgaac
     4561 cagaaagcaa tcgggcagcg tcgtgctgcc cgtgtacata cttttagtcg atggtacgca
     4621 gcagttcgtt aataccaact ttaccgcgag ttttcgcgtc aactttctta acgataactg
     4681 cacagtagag actgtatttg ccatctttcg acggcagatt acctgaaaca accacagacc
     4741 ctgccggaac gcgaccgtag tggatttcgc cggtttcacg gtcgtaaata cgggtgctct
     4801 gaccaatgta tacgcccatg gaaatgacgg aaccttcttc gacaatcacc ccttcaacca
     4861 cttcagagcg tgcgccgatg aagcagttat cttcaataat ggtcgggtta gcctgcagcg
     4921 gctccagcac gccgccgata cccacgccac cggaaaggtg gacgttttta ccaatctgcg
     4981 cacaggaacc gacggtcgcc caggtatcaa ccatggtgcc ttcatcaaca tatgcgccga
     5041 tgttgacgta agacggcatc agcacggtgt tacgggcaat gaacgcgccc tgacgcactg
     5101 ccgctggtgg cacgacgcgg aagccttctt tctggaaacg tgctgcatcg tagttagcga
     5161 atttcatcgg cactttgtcg aagtagcggc tttctgcccc ttcgatcacc tgattatcat
     5221 taatacggaa agagagcagc accgcttttt tcaaccactg atgcgtcacc cactgaccgt
     5281 caattttttc cgctacacgc agtgcgccgg aatccagcag ggcgatcacc tgatttaccg
     5341 cttcgcgggt aacggtgtct gcattggctg gcgtgatctc ggcacggcgt tcaaaagcgg
     5401 tttcaataat gttctgtaac tgctgcattg ttaaactctt ttcatatcag taaacacatc
     5461 accctttatc gtttggattg agggcctctg tcaaccgctg atgcacttcc tgctgcaact
     5521 cgttattaag cgcacgccgg tcagcggtgg caattatgaa taaatcttct actcgctcgc
     5581 caatggttgt aattcgggcg ccatgaagcg aaattcccag atcggcaaaa attttcccga
     5641 ctcgcgccag cagtccaggt tggtcgaggg cgatcagttc gaggaacgat ttgcggtcgg
     5701 tatgggtcgg caaaaacgtc acttcggttt caacagtaaa atggcgcaat ttagcgggtt
     5761 ggcgacgggg ctgcggtggc tgccagctac tttgtgtcag cacctgctcc agaccaaacc
     5821 gaataacctc atgacgatct gcggatagcg ggctaccatc gggttccagc actataaagg
     5881 tatccatcgc cataccgtcg cgagtggtga aaatttgtgc gtcgtgaaca cttaaattgc
     5941 ggcggtctaa ttcggcacag acggcggcaa acagataggg gcggtccggg ctccagataa
     6001 aaatctcggt gcctccacgc gtagcctgcg gactaagcaa taccagcggt ttgcttaaat
     6061 catgctgtaa taaatggcgc gcatgccagg ccagttgatt tgggctatgg cggacaaaat
     6121 agttagcacg acagcgtgac caaatttggt gcagcgcctc ttcgtcgatg ttatccatgc
     6181 gcagcagtgc cagcgcctgg agttggtgat ggcgaacccg ttcgcgcata tccggcgtgt
     6241 tttgcattcc gcgtcgtagc tgtttttcgg tggcaaaata gagttcgcgc aacagacttt
     6301 gcttccagct attccacagt gtttcgttgg tggcgcaaat gtcagccact gtcaggcata
     6361 ccagatagcg cagacgattt tccgtttgaa cttcttcggc aaactgcttg atgacttccg
     6421 ggtcctgaat atcgcggcgc tgagctgtca ccgacatcag caagtgctgg cgaaccagcc
     6481 aggcgaccag ctgtgtttcg cgcgagttaa gcccgtggag ttcggcaaaa tgcaccacgt
     6541 cttgtgcacc gagaatggag tggtcgccgc cacgcccttt ggcgatatcg tgaaacagcg
     6601 cggcgatgaa aatcagctca gttgacggca gacgcggcca gacgtccaca cacaacggat
     6661 ggcgctggcg cgtttcttca ctggcaaaac tctccagttt cagcatcacg cggatagtat
     6721 gttcatccac cgtgtaggca tggaacaggt caaactgcat ctgcccgacg atatgcgacc
     6781 attgcggcat atacgcgccg agcacgctat ggcgatgcat tggcaatagc ccgcgccgca
     6841 ccgctccggg gtgacgcaga atgctcaaaa acagttttcg cgcttccgga atattacaca
     6901 gaggttgttg caggtggcga cgggcgtggc gtaactggcg tagcgtggtg gagtaaatgc
     6961 cggtgatcgc actgttgcgc accatggtgt agaacatacg caagatggct tccggctggc
     7021 gcataaatag tgtttcatca cgcaggtcga tcagcgttcc gcgtagctga aactcatcgt
     7081 cgattggacg gggtttttcg tcggcgggaa gggcgaggat ggcttcatcg aacagttgca
     7141 gcagcatctg gttgagttca ctgacgcggc gtgtaacgcg gaagtaatcc ttcatcatcc
     7201 gctcgaccgg ttcgttacct tcaccactgt aattcaaacg ctgggcgacg ctaagctggc
     7261 gatcgaataa caggcgatta tcgtaacggc tgacgacaag atgcagcgca aagcgaatac
     7321 gccacaatat atgcagacat tcgtttaatt ctgcccgctc cgctgaggtt aagaagccaa
     7381 acccgaccat ttcatccagc gatgttgcgc caaaatgacg gcgggccacc cattgcagag
     7441 tgtggatatc gcgcaagccg ccggggctac ttttgatgtc tggttcaagg ttgtagctgg
     7501 tgccgtggta acgctgatgg cgctggttct gttcttcaac tttcgccgcg tagaacttgt
     7561 cggaaggcca gaatccttcg ctaaaaatat gtttttgcag ttcgaggaac agcgcaacat
     7621 cgccaattaa taagcgggat tcgattaaat tggtggcgac ggttaaatcc gataaccctt
     7681 ccagcatgca ctcttcaagc gtgcgcacgc tatgacccac ttccagcttt acatcccaga
     7741 gcagcgttaa cagctcgccc actttttgcg cctgatcgtc cgggagcttt ttacggctta
     7801 aaatcagtaa atcgatgtca gaaagtggat gcagctcgcc acgaccataa ccaccgacgg
     7861 caaccagtgc caggtcggca atctggctga atcccgcgtc aatccataac cgttgcagga
     7921 gctggtcgat aaactcggta cgcgcctcaa ccaactcttc tgcagagatt ccattgtcaa
     7981 aggcatcacc cagccaacgc tggaaagtat caatatgggc ttttatccca caaacggtta
     8041 attcatcacg gggccagccg catggatttt gcggttgacc gggcagggtg gggagagcgg
     8101 tgtttgcgta ctgttctgga agggtattca ttgtgcgcca cccataagat taaattattg
     8161 cattaaaaaa gccggcattc gccggcttca ttttattcgt cgtgcgagat tatcgccggg
     8221 atggtgtcat ccttacgtag cgtcagaatt tcgcagccgt tatcagtcac cacaatagta
     8281 tgctcgtatt gtgcagacaa gctgcgatct ttggttttta ccgtccagcc atctttcatg
     8341 gtgcggatct cttttttacc cgcgttgacc attggctcga tggtgaacgt catccccggt
     8401 ttcagcacga cgttggtttc acgggagtca tagtgcagca cctgcggttc ttcatggaag
     8461 ccgcgaccaa taccgtgtcc acaatattca cgaacgacag agaagccttc tgcttcgaca
     8521 aacttctgaa tcgccgcacc gatttcgcgc agattaatgc caggttttac catgcgtagc
     8581 gccaggtaca ggctttcttg cgtgatgcgg cacagacgtt cgcccatgat ggtcggctta
     8641 ccgacgataa acattttcga ggtatcgcca tggaaaccat ctttgattac ggtgacatca
     8701 atgttaacga tatcgccatc tttcagcagc ttcgcatcgt ccgggatacc gtggcacacc
     8761 acttcattaa tagagatgca aacggatttc ggatagccgt gatagccgag gcaggcagaa
     8821 accgcgtgtt gttcattaac aatgtaatca ttacagatgc gatccagctc gccggtgctg
     8881 acgcccggtt taacatacgg ttcgatcatc tccagcactt cggcagccag tcggccagcg
     8941 acgcgcattt tttcgatatc ttctggggtc ttgattgaga tagccattaa ttctgtccat
     9001 cagcgtcggt gataccgaca atatatatgt aagtgccgtc aatggtatca cacccgggca
     9061 aattgagaat cattctgaat ttcgccaaac gtgccactga aggttttcta taatagaaaa
     9121 ttcaacgtct gatgctgtac acagcgccaa caattattgg tgtccgcgac gtatttgtgg
     9181 tataaagcgc gccggacttc cgatccattt cgtatacaca gactggacgg aagcgacaat
     9241 ctcactttgt gtaacaacac acacgtatcg gcacatattc cggggtgccc tttggggtcg
     9301 gtaatatggg atacgtggag gcataacccc aacttttata tagaggtttt aatcatggca
     9361 actgtttcca tgcgcgacat gctcaaggct ggtgttcact tcggtcacca gacccgttac
     9421 tggaacccga aaatgaagcc gttcatcttc ggtgcgcgta acaaagttca catcatcaac
     9481 cttgagaaaa ctgtaccgat gttcaacgaa gctctggctg aactgaacaa gattgcttct
     9541 cgcaaaggta aaatcctttt cgttggtact aaacgtgctg caagcgaagc ggtgaaagac
     9601 gctgctctga gctgcgacca gttcttcgtg aaccatcgct ggctgggcgg tatgctgact
     9661 aactggaaaa ccgttcgtca gtccatcaaa cgtctgaaag acctggaaac tcagtctcag
     9721 gacggtactt tcgacaagct gaccaagaaa gaagcgctga tgcgcactcg tgagctggag
     9781 aaactggaaa acagcctggg cggtatcaaa gacatgggcg gtctgccgga cgctctgttt
     9841 gtaatcgatg ctgaccacga acacattgct atcaaagaag caaacaacct gggtattccg
     9901 gtatttgcta tcgttgatac caactctgat ccggacggtg ttgacttcgt tatcccgggt
     9961 aacgacgacg caatccgtgc tgtgaccctg tacctgggcg ctgttgctgc aaccgtacgt
    10021 gaaggccgtt ctcaggatct ggcttcccag gcggaagaaa gcttcgtaga agctgagtaa
    10081 taaggcacgc tcgtacgagc ccttatagac caggtagtac acgtttggtt agggggcctg
    10141 catatggccc cctttttcac ttttatatct gtgcggttta atgccgggca gatcacatct
    10201 ccgaggattt tagaatggct gaaattaccg catccctggt aaaagagctg cgtgagcgta
    10261 ctggcgcagg catgatggat tgcaaaaaag cactgactga agctaacggc gacatcgagc
    10321 tggcaatcga aaacatgcgt aagtccggtg ctattaaagc agcgaaaaaa gcaggcaacg
    10381 ttgctgctga cggcgtgatc aaaaccaaaa tcgacggcaa ctacggcatc attctggaag
    10441 ttaactgcca gactgacttc gttgcaaaag acgctggttt ccaggcgttc gcagacaaag
    10501 ttctggacgc agctgttgct ggcaaaatca ctgacgttga agttctgaaa gcacagttcg
    10561 aagaagaacg tgttgcgctg gtagcgaaaa ttggtgaaaa catcaacatt cgccgcgttg
    10621 ctgcgctgga aggcgacgtt ctgggttctt atcagcacgg tgcgcgtatc ggcgttctgg
    10681 ttgctgctaa aggcgctgac gaagagctgg ttaaacacat cgctatgcac gttgctgcaa
    10741 gcaagccaga attcatcaaa ccagaagatg tatccgctga agtggtagaa aaagaatacc
    10801 aggtacagct ggatatcgcg atgcagtctg gtaagccgaa agaaatcgca gagaaaatgg
    10861 ttgaaggccg catgaagaaa ttcaccggcg aagtttctct gaccggtcag ccgttcgtta
    10921 tggaaccaag caaaactgtt ggtcagctgc tgaaagagca taacgctgaa gtgactggct
    10981 tcatccgctt cgaagtgggt gaaggcatcg agaaagttga gactgacttt gcagcagaag
    11041 ttgctgcgat gtccaagcag tcttaattat cgaaaaggag ccgcctgagg gcggcttctt
    11101 tttgtgccca tcttgtaaat tcagctaacc cttgtggggc tgcgctgaaa agcgacgtac
    11161 aatgtcgcta gtattaattc atttcaatcg ttgacagtct caggaaagaa acatggctac
    11221 caatgcaaaa cccgtctata aacgcattct gcttaagttg agtggcgaag ctctgcaggg
    11281 cactgaaggc ttcggtattg atgcaagcat actggatcgc atggctcagg aaatcaaaga
    11341 actggttgaa ctgggtattc aggttggtgt ggtgattggt gggggtaacc tgttccgtgg
    11401 cgctggtctg gcgaaagcgg gtatgaaccg cgttgtgggc gaccacatgg ggatgctggc
    11461 gaccgtaatg aacggcctgg caatgcgtga tgcactgcac cgcgcctatg tgaacgctcg
    11521 tctgatgtcc gctattccct tgaatggcgt gtgcgacagc tacagctggg cagaagctat
    11581 cagcctgttg cgcaacaacc gtgtggtgat cctctccgcc ggtacaggta acccgttctt
    11641 taccaccgac tcagcagcgt gcctgcgtgg tatcgaaatt gaagccgatg tggtgctgaa
    11701 agcaaccaaa gttgacggcg tgtttaccgc tgatccggcg aaagatccaa ccgcaaccat
    11761 gtacgagcaa ctgacttaca gcgaagtgct cgaaaaagag ctgaaagtca tggacctggc
    11821 ggccttcacg ctggctcgtg accataaatt accgattcgt gttttcaaca tgaacaaacc
    11881 gggtgcgctg cgccgtgtgg taatgggtga aaaagaaggg actttaatca cggaataatt
    11941 cccgtgatgg ataaataagg gtaagattcc gcgtaagtat cgcgggggat cttacccgta
    12001 atatgtttaa tcagggctat acttagcaca cttccactgt gtgtgactgt ctggtctgac
    12061 tgagacaagt tttcaaggat tcgtaacgtg attagcgata tcagaaaaga tgctgaagta
    12121 cgcatggaca aatgcgtaga agcgttcaaa acccaaatca gcaaaatacg cacgggtcgt
    12181 gcttctccca gcctgctgga tggcattgtc gtggaatatt acggcacgc
//
LOCUS       DAMZFT010000138        12198 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.138, whole genome shotgun sequence.
ACCESSION   DAMZFT010000138 DAMZFT010000000
VERSION     DAMZFT010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12198)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12198)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12198
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.138"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..776
                     /locus_tag="PN977_004066"
     CDS             <1..776
                     /locus_tag="PN977_004066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="U32 family peptidase C-terminal
                     domain-containing protein"
                     /protein_id="HDI6044044.1"
                     /translation="CRWEYNVQEGKEDDVGNIVHKYEPIPVQNVEPTLGIGAPTDKVF
                     MIEEAQRPGEYMTAFEDEHGTYIMNSKDLRAIAHVERLTKMGVHSLKIEGRTKSFYYC
                     ARTAQVYRKAIDDAAAGKPFDTSLLETLEGLAHRGYTEGFLRRHTHDDYQNYEYGYSV
                     SDRQQFVGEFTGERKGDLAAVAVKNKFSVGDSLELMTPQGNINFTLEHMENAKGEAMP
                     VAPGDGYTVWIPVPQDLELNYALLMRNFSGETTRNPHGK"
     gene            complement(1106..1423)
                     /locus_tag="PN977_004067"
     CDS             complement(1106..1423)
                     /locus_tag="PN977_004067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310918.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6044045.1"
                     /translation="MKKIAAISLVSVFLMSGCAVHNDETSIGKFGLAYKSNIQRKLDN
                     QYYTEAEASLARGRISGAENIVKNDAVHFCVTQGKKMQIVKLKTEGAGLHGVARLTFK
                     CGE"
     gene            1836..2735
                     /gene="yegS"
                     /locus_tag="PN977_004068"
     CDS             1836..2735
                     /gene="yegS"
                     /locus_tag="PN977_004068"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid kinase YegS"
                     /protein_id="HDI6044046.1"
                     /translation="MAEFPASLLILNGKSTDNLPLREAIMLLREEGMTIHVRVTWEKG
                     DAARYVEEARKLGVATVIAGGGDGTINEVSTALIQCEGDDIPALGILPLGTANDFATS
                     VGIPEALDKALKLAIAGNAIAIDMAQVNKQTCFINMATGGFGTRITTETPEKLKAALG
                     GVSYIIHGLMRMDTLQPDRCEIRGENFHWQGDALVIGIGNGRQAGGGQQLCPNALIND
                     GLLQLRIFTGDEILPALVSTLKSDEDNPNIIEGASSWFDIQAPHEITFNLDGEPLSGQ
                     NFHIEILPAALRCRLPPDCPLLR"
     gene            complement(2817..3590)
                     /locus_tag="PN977_004069"
     CDS             complement(2817..3590)
                     /locus_tag="PN977_004069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020238881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HDI6044047.1"
                     /translation="MNSFERRNKIIQLVNEQGTVLVQDLAGVFAASEATIRADLRFLE
                     QKGVVTRFHGGAAKIMSGNSETETQEVGFKERFQLASAPKNRIAQAAVKMIHEGMTVI
                     LDSGSTTMLIAEGLMTAKNITVITNSLPAAFALSENKDITLVVCGGTVRHKTRSMHGS
                     IAERSLQDINADLMFVGADGIDAVNGITTFNEGYSISGAMVTAANKVIAVLDSSKFNR
                     RGFNQVLPIEKIDIIITDDAVSEVDKLALQKTRVKLITV"
     gene            complement(3696..4736)
                     /gene="gatD"
                     /locus_tag="PN977_004070"
     CDS             complement(3696..4736)
                     /gene="gatD"
                     /locus_tag="PN977_004070"
                     /EC_number="1.1.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HDI6044048.1"
                     /translation="MKSVVNDTDGIVRVAESVIPEIKHQDEVRVKIASSGLCGSDLPR
                     IFKNGAHYYPITLGHEFSGYIDAVGSGIDDLHPGDAVACVPLLPCFTCPECLKGFYSQ
                     CAKYDFIGSRRDGGFAEYIVVKRNNVFALPADMPIEDGAFIEPITVGLHAFHLAQGCE
                     NKNVIIIGAGTIGLLAIQCAVALGAKSVTAIDISSEKLALAKSFGAMQTFNSREMSAP
                     QIQGVLRELRFNQLILETAGVPQTVELAVEIAGPHAQLALVGTLHQDLHLTSATFGKI
                     LRKELTVIGSWMNYSSPWPGQEWETASRLLTERKLSLEPLIAHRGSFESFAQAVRDIA
                     RNAMPGKVLLIP"
     gene            complement(4784..6139)
                     /locus_tag="PN977_004071"
     CDS             complement(4784..6139)
                     /locus_tag="PN977_004071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HDI6044049.1"
                     /translation="MFSEVMRYILDLGPTVMLPIVIIIFSKILGMKAGDCFKAGLHIG
                     IGFVGIGLVIGLMLDSIGPAAKAMAENFDLNLHVVDVGWPGSSPMTWASQIALVAIPI
                     AILVNVAMLLTRMTRVVNVDIWNIWHMTFTGALLHLATGSWMIGMAGVVIHAAFVYKL
                     GDWFARDTRNFFELEGIAIPHGTSAYMGPIAVLVDAIIEKIPGVNRIKFSADDIQRKF
                     GPFGEPVTVGFVMGLIIGILAGYDVKGVLQLAVKTAAVMLLMPRVIKPIMDGLTPIAK
                     QARSRLQAKFGGQEFLIGLDPALLLGHTAVVSASLIFIPLTILIAVCVPGNQVLPFGD
                     LATIGFFVAMAVAVHRGNLFRTLISGVIIMSITLWIATQTIGLHTQLAANAGALKAGG
                     MVASMDQGGSPITWLLIQVFSPQNIPGFIIIGAIYLTGIFMTWRRARGFIKQEKAVLA
                     E"
     gene            complement(6143..6427)
                     /gene="gatB"
                     /locus_tag="PN977_004072"
     CDS             complement(6143..6427)
                     /gene="gatB"
                     /locus_tag="PN977_004072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIB"
                     /protein_id="HDI6044050.1"
                     /translation="MKRKIIVACGGAVATSTMAAEEIKELCQSHNIPVELIQCRVNEI
                     ETYMDGVHLICTTARVDRSFGDIPLVHGMPFVSGVGIEALQNKILTILQG"
     gene            complement(6458..6910)
                     /gene="gatA"
                     /locus_tag="PN977_004073"
     CDS             complement(6458..6910)
                     /gene="gatA"
                     /locus_tag="PN977_004073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIA"
                     /protein_id="HDI6044051.1"
                     /translation="MTNLFVRSGISFVDRSEVLAHIGNEMLAKGVVHDTWPQALIARE
                     AEFPTGIMLEQHAIAIPHCEAIHAKSSAIYLLRPTNKVHFQQADDDNDVAVSLVIALI
                     VENPQQQLKLLRCLFGKLQQPDIVETLITLPETQLKEYFTKYVLDSDE"
     gene            complement(6920..8182)
                     /gene="gatZ"
                     /locus_tag="PN977_004074"
     CDS             complement(6920..8182)
                     /gene="gatZ"
                     /locus_tag="PN977_004074"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatZ"
                     /protein_id="HDI6044052.1"
                     /translation="MKTLIARHKAGEHIGICSVCSAHPLVIEAALAFDRNSTRKVLIE
                     ATSNQVNQFGGYTGMTPADFREFVFAIANKVGFARERIILGGDHLGPNCWQQENADAA
                     MEKSVELVKAYVRAGFSKIHLDASMSCAGDPIPLAPETVAERAAVLCLAAESVATDCQ
                     REQLSYVIGTEVPVPGGEASAIQSVHITQVEDAANTLRTHQKAFIARGLAEALTRVIA
                     IVVQPGVEFDHSNIIHYQAQEAQALAQWIEKTKMVYEAHSTDYQTQAAYRELVRDHFA
                     ILKVGPALTFALREAVFALAQIEQELIAPENRSGCLAVIEEVMLDEPQYWKKYYRTGF
                     HDSLLDIRYSLSDRIRYYWPHSRIKNSVETMMVNLEGVDIPLGMISQYLPKQFERIQS
                     GELSAIPHQLIMDKIYDVLRAYRYGCAE"
     gene            complement(8211..9065)
                     /gene="gatY"
                     /locus_tag="PN977_004075"
     CDS             complement(8211..9065)
                     /gene="gatY"
                     /locus_tag="PN977_004075"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416599.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatY"
                     /protein_id="HDI6044053.1"
                     /translation="MYVVSTKQMLNNAQRGGYAVPAFNIHNLETMQVVVETAASMHAP
                     VIIAGTPGTFTHAGTENLMALVSAMAKQYHHPLAIHLDHHTKFDDIAQKVRSGVRSVM
                     IDASHLPFAQNISRVKEVVDFCHRFDVSVEAELGQLGGQEDDVQVNEADALYTNPVQA
                     REFAEATGIDSLAVAIGTAHGMYASAPALDFSRLENIRQWVNLPLVLHGASGLSTKDI
                     QQTIKLGICKINVATELKNAFSQALKNYLTEHPEATDPRDYLQSAKFAMRDVVSKVIA
                     DCGCEGRA"
     gene            complement(9294..10346)
                     /gene="fbaB"
                     /locus_tag="PN977_004076"
     CDS             complement(9294..10346)
                     /gene="fbaB"
                     /locus_tag="PN977_004076"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HDI6044054.1"
                     /translation="MTDIAQLLGKDADNLLQHRCMTIPSDQLYLPGHDYVDRVMIDNN
                     RPPAVLRNMQTLYNTGRLAGTGYLSILPVDQGVEHSAGASFAANPLYFDPKNIVELAI
                     EAGCNCVASTYGVLASVSRRYAHRIPFLVKLNHNETLSYPNTYDQTLYASVEQAFNMG
                     AVAVGATIYFGSEESRRQIEEISAAFERAHELGMVTVLWAYLRNSAFKKDGVDYHVSA
                     DLTGQANHLAATIGADIVKQKMAENNGGYKAINYGYTDDRVYSKLTSENPIDLVRYQL
                     ANCYMGRAGLINSGGAAGGETDLSDAVRTAVINKRAGGMGLILGRKAFKKSMADGVKL
                     INAVQDVYLDSKITIA"
     gene            10603..11880
                     /locus_tag="PN977_004077"
     CDS             10603..11880
                     /locus_tag="PN977_004077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDI6044055.1"
                     /translation="MKTTAKLSFMMFVEWFIWGAWFVPLWLWLSKSGFSAGEIGWSYA
                     CTAIAAILSPILVGSITDRFFSAQKVLAVLMFAGAVLMYFAAQQTTFAGFFPLLLAYS
                     LTYMPTIALTNSIAFANVPDVERDFPRIRVMGTIGWIASGLACGFLPQMLGYADISPT
                     NIPLLITAGSSALLGVFAFFLPDTPPKSTGKMDIKVMLGLDALILLRDKNFLVFFFCS
                     FLFAMPLAFYYIFANGYLTEVGMKNATGWMTLGQFSEIFFMLALPFFTKRFGIKKVLL
                     LGLVTAAIRYGFFIYGSADEYFTYALLFLGILLHGVSYDFYYVTAYIYVDKKAPVHMR
                     TAAQGLITLCCQGFGSLLGYRLGGVMMEKMFAYQEPVNGLTFNWAGMWTFGAVMIAII
                     AVLFMIFFRESDNEITAIKVDDRDIALTQGEVK"
     gene            11877..>12198
                     /locus_tag="PN977_004078"
     CDS             11877..>12198
                     /locus_tag="PN977_004078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HDI6044056.1"
                     /translation="MKTERILGALYGQALGDAMGMPSELWPRSRVKAHFGWIDRFLPG
                     PKENNAACYFNRAEFTDDTSMALCLADALLEREGKIDPDLIGRNILDWALRFDAFNKN
                     VLGPT"
BASE COUNT         3084 a         3131 c         2826 g         3157 t
ORIGIN      
        1 cctgccgctg ggagtacaac gtccaggaag ggaaagaaga tgacgttggc aacatcgtgc
       61 acaagtacga gccgattccg gtgcaaaatg ttgagccgac gctgggcatc ggcgcgccaa
      121 ccgacaaagt gtttatgatc gaagaagctc agcgtccggg cgagtatatg actgcgtttg
      181 aagatgagca cggcacttac atcatgaact cgaaagatct gcgcgctatc gcccacgttg
      241 aacgcctgac caaaatgggc gtgcattcgc tgaaaatcga aggtcgtaca aaatctttct
      301 actattgcgc acgcaccgcg caggtttacc gtaaagctat cgatgacgca gctgcgggaa
      361 aaccattcga taccagcctg ctggaaactc tggaaggtct ggcgcatcgt ggctataccg
      421 aaggtttcct gcgtcgtcat actcacgacg attatcagaa ctacgaatac ggttattcag
      481 tttctgaccg ccagcagttt gttggtgagt ttaccggtga gcgcaagggc gacctcgcgg
      541 cggtagcggt gaaaaataaa ttctccgttg gcgacagcct tgagctgatg acgccgcaag
      601 ggaacattaa ctttaccctt gagcatatgg aaaacgccaa aggcgaagca atgccggtcg
      661 caccgggcga tggttatact gtgtggatcc cggtgccgca ggatcttgag ctaaattacg
      721 cgctgctgat gcgtaatttc tccggggaaa ccacgcgtaa cccccacggt aagtgattaa
      781 tttcgattat ttttcccgga tggaaaattc ttagaaaccg atcacataca gctgcattta
      841 ttaaggttat catccgtttc gctgaaaaac ataacccata aaatgctagc tgtaccagga
      901 accacctcct tagcctgtgt aatctccctt acacgggctt attttttatg cttaagccct
      961 atctctggta accgtcttcc attgaccaca tcgatagaat ctcccttcat agcacgatgc
     1021 ctttcactta ttggcatcgt gctcgcacag gttccggtta cgcacagcca gaacgcgcat
     1081 gtttgacgct taccaaaaaa tattctcact ctccacattt gaatgtcaga cgagcgacgc
     1141 catgtaatcc tgcaccttct gtcttcaatt taactatctg catttttttg ccctgtgtaa
     1201 cacagaaatg aactgcatca ttttttacta tattttctgc accagatatt ctacccctgg
     1261 ctaaagaagc ttcggcttcg gtgtagtatt ggttatcgag tttacgctga atattacttt
     1321 tatatgcaag gccaaattta ccgatacttg tttcatcatt atgtacagca caaccagaca
     1381 taagaaaaac actaactaat gatatagcag ctatcttttt catcttacct tcccccatta
     1441 aataccaacg acacgctctg gtgtttaaat ataataatgg catgattatt ataattgaat
     1501 aggattataa taaatgttct gtacaacatt tcctacataa gtaggaatta cagacacgga
     1561 tgcccttccg ggaaacccca tgggggggta atatattttt aatagtatat tgaagatgcc
     1621 actgttcagt tgaatattag gtatatgttc ttttttgaaa tttatcggtg gctaccgtta
     1681 acattcactg tccccattgc aagctcctgg tggtaagcac tgaatcccca tacctgaact
     1741 gactttttat cctccgactt tcatcctgtt ctgactccac cttttctttt ctgctctaca
     1801 ctatctacag accaatcata aaggcacata cgatcatggc agaatttccc gccagcttac
     1861 tgattcttaa tggcaaaagt actgacaatc tacccctgcg cgaagcaatt atgctgttgc
     1921 gtgaggaagg aatgacgatc catgtgcggg tcacctggga gaaaggcgat gccgcacgat
     1981 atgtagagga ggcccggaag ttgggcgtcg caacggtgat tgccggtggt ggcgatggca
     2041 ccattaatga agtttctacg gcgttgattc agtgtgaggg ggatgacata cccgcgctgg
     2101 gaattttgcc attaggaacc gccaatgatt ttgccaccag tgtagggatt cctgaggcac
     2161 tggataaggc gctgaaactg gcaattgccg gtaacgccat tgcgatagat atggcgcagg
     2221 tcaacaaaca aacctgtttt attaatatgg cgacaggcgg atttgggacg cgtattacca
     2281 cagaaacgcc ggaaaaatta aaagccgcgc tgggtggcgt ctcttacatc attcatggct
     2341 taatgcgcat ggatactctg caaccggacc gttgtgaaat ccgcggtgaa aactttcact
     2401 ggcaaggtga cgccctggtc attggtattg gtaacgggcg tcaggctggt ggcggtcagc
     2461 aattgtgccc gaacgcgtta attaacgatg gcttgctgca actgcgcatt tttaccggcg
     2521 atgaaattct tccggctctc gtatcaacct taaaatctga cgaagataac ccgaatatta
     2581 tcgaaggcgc ttcgtcgtgg tttgatatac aagccccaca cgaaatcact tttaatcttg
     2641 atggcgaacc gttgagcgga caaaactttc atattgaaat acttccggcg gcgttgcgtt
     2701 gtcgattacc accggattgt ccattattgc gttaatcaaa ctattctctc ttgaaccagg
     2761 gagaatccgt aatgccggat gcggtgtaaa caccttatcc ggcatacaga acaatactac
     2821 accgtaatta attttacccg tgtcttctgc aatgccagtt tatccacctc agacacagca
     2881 tcatcggtaa taataatgtc aattttttcg attggcagta cctgattaaa accgcgacga
     2941 ttaaatttcg atgaatcgag aacggcaatc actttatttg cggctgtgac catcgccccg
     3001 ctaatcgaat agccttcatt aaaggtcgta ataccattaa ccgcatcaat accatcagca
     3061 ccgacaaaca ttaaatcggc attaatatcc tgcaatgaac gctcagcaat ggagccatgc
     3121 atcgagcgcg ttttatggcg gaccgtgcca ccgcagacga ccagagtaat gtctttattt
     3181 tcggaaaggg caaatgctgc cgggagactg ttggtaatca ccgtgatatt tttggcggtc
     3241 attaatcctt cggcgataag cattgtcgta cttccgctgt cgagaataac ggtcatccct
     3301 tcgtggatca ttttgactgc cgcctgcgct attctgtttt ttggcgcgct ggcgagctga
     3361 aatcgctctt taaacccgac ttcctgggtc tcggtttcac tattaccgga cattattttc
     3421 gccgcaccgc catgaaagcg cgtaaccacg cctttttgtt cgagaaagcg caaatcggca
     3481 cggattgtcg cttccgaggc agcaaatact cccgccagat cctgaacaag cacggttccc
     3541 tgttcattca ctaattggat gatcttattc cttcgctcga atgaattcat cgtcatttac
     3601 ccatattcaa ttgtggctag tgtaaacgaa gggggaccat tggtgaatga tctgttttgc
     3661 acaataccgg gccagcatca cgctggcccg cggtttcagg gaatgagcaa cactttgccc
     3721 ggcatagcat tacgagcgat gtcacgcacc gcctgggcga agctttcaaa gcttccacgg
     3781 tgagcgatta atggctccag gcttaactta cgttctgtca gcaaccggct cgccgtttcc
     3841 cactcctgcc ccggccaagg gctggagtag ttcatccaac tgccaatcac cgtcagctct
     3901 ttacgcaata ttttgccaaa cgttgccgat gttaaatgca gatcctgatg caacgtgccc
     3961 accagcgcca gttgggcatg aggaccggca atctctaccg ccagttcgac ggtttgcggg
     4021 acgccagccg tctcgaggat aagctgatta aagcgcagct cgcgtaaaac gccctgtatt
     4081 tgcggcgcgc tcatttcacg gctgttaaat gtttgcatcg caccgaaaga ttttgccagt
     4141 gccagttttt ctgagctaat gtcgatagcc gtcacactct ttgctcccag cgcgacagcg
     4201 cactgaattg ccagcaggcc aatggttccg gcaccaataa taataacgtt tttattctca
     4261 catccttgcg ctaaatggaa cgcatgcagg ccaacggtaa tcggttcaat aaaagcgcca
     4321 tcctcaatag gcatatccgc aggtagtgca aagacattgt ttcgcttaac gacaatatat
     4381 tcggcaaatc caccatcacg ccgtgagcca ataaaatcat attttgcgca ctgggaataa
     4441 aaccctttca gacactctgg acaagtaaaa cagggtaata acggcacaca ggcaaccgca
     4501 tcgccgggat gtaaatcatc aataccggat cccaccgcat caatatagcc gctaaattca
     4561 tggcctaacg ttattggata ataatgtgca ccatttttaa atatcctggg taaatcggaa
     4621 ccacataagc ccgagctggc aatttttacc cgcacctcat cctgatgttt aatttcagga
     4681 atgacgcttt ctgctacgcg cacgatacca tcagtatcat tcaccactga tttcataaaa
     4741 actcctgatt gttaaggggg ataacctccc cctcaggtaa aaattattct gcgagaacgg
     4801 ctttctcttg tttaataaaa ccacgcgctc tacgccaggt catgaaaata ccggtcagat
     4861 aaattgcgcc gataataatg aaaccgggaa tattttgcgg ggagaaaacc tgaatcagta
     4921 accaggtaat gggagaaccg ccctgatcca ttgaagccac catacccccg gcttttaacg
     4981 cgcccgcatt agccgccagt tgggtgtgca aaccaatagt ttgcgtcgcg atccacaggg
     5041 tgatgctcat aatgatgaca cccgagatta aggtgcggaa cagatttcca cgatgcaccg
     5101 cgaccgccat cgccacaaag aagccgatgg tggcaaggtc gccaaacggc agcacctgat
     5161 tacccggcac acaaacagca attaaaatgg tgagcgggat aaaaatcagg cttgccgata
     5221 ccaccgccgt atgccccagc agtaatgcgg gatccaggcc aatcaggaac tcctgaccgc
     5281 cgaacttcgc ctgtaaacga ctacgggcct gcttagcgat gggcgttaaa ccatccatga
     5341 tgggtttaat cacccgtggc atcagcagca taaccgccgc cgtttttacc gccagctgca
     5401 atacaccttt gacatcgtaa cccgcgagga tgccgataat cagccccatc acaaaaccca
     5461 cggtgacagg ctcgccaaac ggaccaaatt tgcgctgaat atcgtcggca ctaaatttaa
     5521 ttcggttaac gcctgggatt ttctcgatga tagcatccac cagcaccgca attggcccca
     5581 tatacgccga cgtaccgtgc gggatggcaa tgccttccag ctcaaagaaa tttcgggtat
     5641 cgcgggcaaa ccagtcgccg agcttataaa caaacgccgc gtgaattacc acacccgcca
     5701 tccctatcat ccatgaaccg gttgccagat gcagcaacgc gccggtgaag gtcatatgcc
     5761 agatattcca gatatcaaca tttaccaccc gcgtcatacg ggtcagtaac atcgccacgt
     5821 taaccagaat cgcaatcgga atcgccacca gcgcaatttg cgacgcccag gtcatgggtg
     5881 aagagcccgg ccagccgaca tcgaccacat gcagattcag gtcgaaattt tccgccatcg
     5941 ctttcgccgc cggaccaatg gaatccagca ttaagccaat cacaaggcca atgccaacaa
     6001 agccaatccc gatatgcaga cccgctttaa agcaatcgcc tgccttcatg cctaatattt
     6061 tagaaaaaat aatgatgaca atcggcagca tcaccgtagg gccgaggtcg agaatataac
     6121 gcatgacttc tgaaaacata ggtcacccct gtaagatagt cagaatttta ttttgtaatg
     6181 cttcgatacc gacaccagaa acaaaaggca tgccgtgaac taacggaata tcgccaaaac
     6241 tacgatccac cctggccgtg gtgcatatca aatgcacgcc atccatatag gtttctattt
     6301 cattaacccg acactggatt aattcaacag gaatattatg actctgacac aactctttaa
     6361 tttcttccgc cgccatcgtg gaggtcgcaa ccgcgcctcc gcaagcgaca ataatcttgc
     6421 gtttcataaa cagtcctttt tattgttaca gagggattta ttcgtctgaa tctaaaacat
     6481 actttgtgaa gtattccttt aactgagttt caggaagagt aattagtgtc tcgacgatat
     6541 cgggctgttg taacttgcca aataaacagc gtaaaagttt caattgctgc tgcggatttt
     6601 ccacaatcaa cgcaataacc aacgataccg ccacgtcgtt atcatcatcc gcttgctgaa
     6661 aatgaacttt atttgttggc cttaacagat aaatggctga cgacttagca tgaatcgcct
     6721 cacaatgcgg tattgcaatg gcgtgctgct caagcattat cccggtaggg aattctgctt
     6781 ctctggcaat taatgcctgt ggccaggtat catgaaccac acctttcgcg agcatctcat
     6841 taccgatatg ggctaaaact tcgctacgat cgacaaaaga aattccgcta cgaacaaaca
     6901 ggttagtcat ataccgtcct tattccgcac agccgtagcg ataggcgcgc aaaacatcat
     6961 aaattttatc cataatcaac tgatgcggta ttgctgataa ttccccggac tgaatgcgtt
     7021 caaattgttt gggaagatac tgactaatca tgcccagtgg gatgtccacg ccttccagat
     7081 tcaccatcat cgtttcgacg ctatttttaa tccggctatg cggccagtaa taacgaatac
     7141 gatccgacag gctgtaacga atatccagta atgaatcgtg aaaacccgtg cgataatatt
     7201 tcttccagta ctgcggttcg tcgagcatta cttcttcaat taccgccagg caaccgctgc
     7261 gattttcggg ggcgataagt tcctgctcaa tttgtgccag cgcaaatacc gcctcacgta
     7321 aagcaaaggt taatgcggga ccgactttca atattgcaaa gtgatcgcgg actaattccc
     7381 gataggccgc ctgagtctgg taatcggtgg aatgcgcttc ataaaccatt ttggtttttt
     7441 ctatccattg cgccagcgcc tgagcttctt gcgcctgata atgaataata ttgctatggt
     7501 caaattccac accaggctgc accacgatgg caatcacgcg tgttaacgcc tctgccagac
     7561 cacgggcaat aaaggccttt tgatgcgtgc gtaaagtatt ggcggcatct tcaacctggg
     7621 tgatgtgtac tgactgaatg gcgctggcct caccacccgg aaccggaact tcggtgccaa
     7681 tgacatagct cagttgctca cgctggcaat ctgtcgccac actttccgcc gccagacaaa
     7741 gcacagcagc tcgttcagca accgtttctg gggctaacgg tataggatcc cccgcgcagg
     7801 acattgacgc atcaagatga attttactga agccggcacg aacatatgcc tttaccagct
     7861 cgacggattt ttccatcgcc gcatccgcat tttcttgctg ccagcagttt ggccccagat
     7921 gatcgccgcc gagaataatg cgttcgcgtg caaacccaac tttattggca atcgcaaaaa
     7981 caaattcgcg aaagtctgcc ggtgtcattc cggtataacc gccaaattga ttgacctggt
     8041 ttgacgttgc ttcaatcagc actttgcgcg tgctgttgcg atcaaatgcc agcgccgctt
     8101 cgataaccaa cggatgggca gaacagactg aacatatgcc gatatgttca ccagctttat
     8161 gccgggcaat taacgttttc atattttttc cttgttaaat ggcaggtgcg ttatgccctg
     8221 ccctcgcagc cacaatcggc aatcactttg ctcaccacat cgcgcattgc gaatttagcc
     8281 gactgcaaat aatcccgggg atcggtcgct tcggggtgct cggtcaggta attttttaac
     8341 gcctgcgaga aggcattttt cagctccgtt gcaacgttga ttttgcatat ccccagtttg
     8401 atggtttgct gaatatcctt agtcgataat cctgacgcac catgcagcac cagcggtaag
     8461 ttcacccact ggcgaatgtt ctccagtcga gaaaaatcaa gcgccggtgc gctggcatac
     8521 ataccgtgag ccgtgccgat ggcgaccgcc agggaatcaa tcccggttgc ctcggcaaat
     8581 tcacgcgcct gaacggggtt ggtgtacaac gcatcggctt cattgacttg cacatcatct
     8641 tcctggccgc caagttgccc cagctccgct tcgacgctga catcaaagcg atggcaaaaa
     8701 tccaccacct ctttgacccg tgaaatattt tgcgcaaaag gcaaatgcga ggcgtcaatc
     8761 atgactgagc gcacgccaga acgaaccttc tgagcgatat cgtcaaattt cgtgtgatgg
     8821 tcgagatgaa tcgccagtgg atggtgatat tgcttcgcca tcgcgctgac cagcgccatc
     8881 agattttctg taccggcatg agtaaatgtg ccgggcgttc cggcgatgat gaccggcgca
     8941 tgcatgctgg cagcagtttc caccaccact tgcatcgttt cgagattgtg aatattgaat
     9001 gccggaaccg cataaccgcc gcgctgcgcg ttgttcagca tctgctttgt cgataccacg
     9061 tacattttca tatcctgtcg tttgttttcg atttcaaaat acaatgaaat gatttgtttt
     9121 caaatgtcga gataacgatc acaaaaacga caatgtgaaa attaatcaga attacaggca
     9181 aaaaaaacgg cctcccgtta gggaagccgt agcaaagtgc gcgtgttttt atgccggatg
     9241 caacgtaaac gccttatccg ccctacgggg tagtgctgtt cgtatgaggc gtatcaggca
     9301 atagtaattt tgctatcgag ataaacgtcc tgcacggcgt taatcagttt cacgccgtca
     9361 gccatcgatt tcttgaacgc cttacgtcca agaatcagcc ccattccgcc agcgcgtttg
     9421 ttgataaccg cagtacgcac tgcatcgctg aggtcagttt caccgcccgc agcaccgccg
     9481 gagtttatca acccagcccg gcccatatag cagttagcta actgataacg caccagatca
     9541 atcgggtttt cgctggtcaa tttgctgtag acgcgatcgt cggtataacc gtaattaatc
     9601 gctttatagc cgccgttatt ttccgccatt ttttgtttga cgatatctgc acctatggtt
     9661 gccgccagat ggttcgcctg accagtcagg tcggcggaaa catggtaatc aacgccatct
     9721 ttcttaaagg cggagttacg caaataggcc cacagcactg tcaccatgcc cagctcgtgc
     9781 gcacgttcaa aagccgcaga aatttcttca atctggcgac gtgactcttc cgagccaaaa
     9841 tagatagtcg ccccaaccgc caccgcgccc atattgaacg cctgctccac gctggcatac
     9901 agcgtttgat cgtaggtatt cgggtaactt agcgtctcgt tgtgattaag tttgacgagg
     9961 aatggaatgc gatgcgcgta acgccgcgat accgatgcca gcacgccgta agttgacgcc
    10021 acgcagttac agcccgcttc gatcgccagt tcaacaatgt ttttcgggtc aaagtagagc
    10081 gggttagcgg caaatgaagc tccggcagag tgctcaacgc cctggtcaac cggcagaata
    10141 gaaagatatc ctgtgccagc cagacgcccg gtgttgtaca acgtctgcat attacgtaac
    10201 accgctggcg ggcgattatt gtcaatcatc acgcggtcta cgtagtcatg tccggggaga
    10261 taaagctggt cagaaggaat agtcatacaa cggtgctgta aaaggttgtc ggcgtctttg
    10321 ccaagcaact gcgcaatatc tgtcattact atgctcccgt aaattccgat tggatatcgg
    10381 ctatggattg tcctggcccg ccttttgcgg gcaatcataa tcctggtcgt tacgggcgag
    10441 tttttccatc ttttggatgt tttttcagcg ttttctgttg gctcgattca tcagaaaaaa
    10501 tgttacagcg gtcaagtttt caccgcaaag gtatttaaaa ggtattatta agtggtattg
    10561 tcatcgcgta ccttacatta cctgtcatga aggaattaaa agatgaaaac aacagcaaag
    10621 ctgtcgttca tgatgtttgt tgaatggttt atctggggcg cgtggtttgt gccattgtgg
    10681 ttgtggttaa gtaaaagcgg ttttagtgcc ggagaaattg gctggtcgta tgcctgtacc
    10741 gccattgcgg cgatcctgtc gccgattctg gttggctcca tcactgaccg ctttttctcg
    10801 gcacagaaag tgctggcggt attgatgttc gctggtgcgg tgctgatgta tttcgctgcg
    10861 caacagacca cttttgccgg cttcttcccg ttactgctgg cctactcgct aacctatatg
    10921 ccgaccattg cgctgactaa cagtatcgct tttgccaacg tgccggatgt ggagcgtgat
    10981 ttcccgcgca ttcgtgtgat gggcactatc ggctggattg cctctggtct ggcatgtgga
    11041 ttcttgccgc aaatgctggg ttatgccgat atctcaccga cgaacatccc gctgctgatt
    11101 actgccggaa gttctgctct gcttggtgtg tttgcgtttt tcctgcccga cacgccacca
    11161 aaaagcaccg gcaaaatgga tattaaagtc atgctcggcc tggatgcgct gatcctgctg
    11221 cgcgataaga acttcctcgt ctttttcttc tgttcattcc tgtttgcgat gccactggcg
    11281 ttctattaca tctttgccaa cggttatctg accgaagttg gcatgaaaaa cgccactggc
    11341 tggatgacgc tcggccagtt ctctgaaatc ttctttatgc tggcattgcc gtttttcact
    11401 aaacgctttg gtatcaaaaa ggtattgttg cttggtctgg tcaccgctgc gatccgctat
    11461 ggcttcttta tttacggtag tgcggatgaa tatttcacct acgcgttact gttcctcggc
    11521 attttgctgc acggcgtaag ttacgatttt tactacgtta ccgcttacat ctatgtcgat
    11581 aaaaaagccc ccgtgcatat gcgtaccgct gcgcaggggc tgatcacgct ctgctgccag
    11641 ggcttcggca gtttgctcgg ctatcgtctt ggcggtgtga tgatggaaaa gatgttcgct
    11701 tatcaggaac cggtaaacgg actgactttc aactgggccg ggatgtggac tttcggcgcg
    11761 gtgatgattg ccattatcgc cgtgctgttc atgatttttt tccgcgaatc cgacaacgaa
    11821 attacggcta tcaaggtcga tgatcgcgat attgcgttga cacaagggga agttaaatga
    11881 aaacagaacg tattctcggt gctctttatg ggcaggcgtt aggggatgcg atggggatgc
    11941 cctccgagct ttggccgcgc agtcgcgtca aagcacactt tggctggatt gaccgttttc
    12001 tgcctgggcc aaaggagaat aacgcagcct gttattttaa ccgcgccgaa ttcaccgacg
    12061 atacctcgat ggcgctgtgt ctggcggatg cgttactgga acgtgaaggc aagatcgatc
    12121 cggatctgat tgggcgtaat attctcgact gggcgctgcg tttcgacgcc tttaacaaaa
    12181 acgtactagg tccgacct
//
LOCUS       DAMZFT010000139        12197 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.139, whole genome shotgun sequence.
ACCESSION   DAMZFT010000139 DAMZFT010000000
VERSION     DAMZFT010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12197)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12197)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12197
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.139"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(<1..458)
                     /locus_tag="PN977_004079"
     CDS             complement(<1..458)
                     /locus_tag="PN977_004079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HDI6044057.1"
                     /translation="MSFWKVAAAQYEPRKTSLTEQVAHHLEFVRAAARQQCQLLVFPS
                     LSLLGCDYSRRALPAPPDLSLLDPLCYAATTWRMTIIAGLPVEYNDRFIRGIAVFAPW
                     RKTPGIYHQSYGACLGRRSRTITVVDEQPEGMDMDPTCSLFTIGQCLGEP"
     gene            complement(560..790)
                     /gene="holE"
                     /locus_tag="PN977_004080"
     CDS             complement(560..790)
                     /gene="holE"
                     /locus_tag="PN977_004080"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit theta"
                     /protein_id="HDI6044058.1"
                     /translation="MLKNLAKLDQTEMDKVNVDLAAAGVAFKERYNMPVIAEAVEREQ
                     PEHLRSWFRERLIAHRLASVNLSRLPYEPKLK"
     gene            929..1303
                     /gene="yobA"
                     /locus_tag="PN977_004081"
     CDS             929..1303
                     /gene="yobA"
                     /locus_tag="PN977_004081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopC domain-containing protein YobA"
                     /protein_id="HDI6044059.1"
                     /translation="MASTARSLRYALAILTTSLVTPSVWAHAHLMHQYPAANAQLTAA
                     PQAITLNFSEGVETGFSGAKITGPKNENIKTLPAKRNEQDPKQLIVPLADSLKPGTYT
                     VNWHVVSVDGHKTKGHYTFSVK"
     gene            1307..2179
                     /gene="copD"
                     /locus_tag="PN977_004082"
     CDS             1307..2179
                     /gene="copD"
                     /locus_tag="PN977_004082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis membrane protein CopD"
                     /protein_id="HDI6044060.1"
                     /translation="MLAFTWIALRFIHFTSLMLVFGFAMYGAWLAPLTIRRLLTKRFL
                     RLQQHAAVWSLISATAMLAVQGGLMGTGWSDVFSPNIWQAVLQAQFGGVWLWQIVLAL
                     VTLIVALMQPRNMPRLLFMLTTAQFFLLAGVGHATLNEGVTAKIHQTNHAIHLICAAA
                     WFGGLLPVLWCMQLIKGRWRHQAIQALMRFSWCGHFAVIGVLASGVLNALLITGFPPT
                     LTTYWGQLLLLKAILVMIMVVIALANRYVLVPRMRQDEDRAAPWFVWMTKLEWAIGAV
                     VLVIISLLATLEPF"
     gene            2192..2533
                     /locus_tag="PN977_004083"
     CDS             2192..2533
                     /locus_tag="PN977_004083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebY family protein"
                     /protein_id="HDI6044061.1"
                     /translation="MMKKSILAFLLLTSSAAALAAPQVITVSRFEVGKDKWAFNREEV
                     MLTCRPGNALYVINPSTLVQYPLNDIAQKEVASGKTKAQPISVIQINDPNNPGEKMSL
                     APFIERAEKLC"
     gene            2929..3585
                     /gene="pphA"
                     /locus_tag="PN977_004084"
     CDS             2929..3585
                     /gene="pphA"
                     /locus_tag="PN977_004084"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase"
                     /protein_id="HDI6044062.1"
                     /translation="MKQPAPVYQRIAGHQWRHIWLSGDIHGCLEQLRRKLWHCRFDPW
                     RDLLISVGDVIDRGQQSLRCLQLLEQHWVRAVRGNHEQMAMDALASQQMSLWLMNGGN
                     WFIALADYQQKQAKTALEKCQYLPFILEVHSRTGKHVIAHADYPDDVYEWQKDVDLHQ
                     VLWSRSRLGERQKGQGITGADHFWFGHTPLRHRVDIGNLHYIDTGAVFGGELTLVQLQ
                     "
     gene            complement(3586..3777)
                     /locus_tag="PN977_004085"
     CDS             complement(3586..3777)
                     /locus_tag="PN977_004085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebW family protein"
                     /protein_id="HDI6044063.1"
                     /translation="MFALVLFVCYLDGGCEDIVVDVYNTEQQCLYSMSDQRIRHGGCF
                     PIEDFIDGFWRPAQEYGDF"
     gene            complement(3882..4118)
                     /locus_tag="PN977_004086"
     CDS             complement(3882..4118)
                     /locus_tag="PN977_004086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1480 family protein"
                     /protein_id="HDI6044064.1"
                     /translation="MKTSVRIGAFEIDDGELHGESPGDRTLTIPCKSDPDLCMQLDAW
                     DAETSIPALLNGEHSVLYRTRYDQQSDAWIMRLA"
     gene            complement(4236..5675)
                     /gene="rsmF"
                     /locus_tag="PN977_004087"
     CDS             complement(4236..5675)
                     /gene="rsmF"
                     /locus_tag="PN977_004087"
                     /EC_number="2.1.1.178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1407)-C(5))-methyltransferase
                     RsmF"
                     /protein_id="HDI6044065.1"
                     /translation="MAQHTVYFPDAFLTQMREAMPSTLSFDDFLAACQRPLRRSIRVN
                     TLKISVADFLQLTAPYGWTLTPIPWCEEGFWIERDDEDALPLGSTAEHLSGLFYIQEA
                     SSMLPVAALFADGNAPQRVMDVAAAPGSKTTQIAARMNNEGAILANEFSASRVKVLHA
                     NISRCGISNVALTHFDGRVFGAAVPEMFDAILLDAPCSGEGVVRKDPDALKNWSPESN
                     QEIAATQRELIDSAFHALRPGGTLVYSTCTLNREENEAVCLWLKETYPDAVEFLPLGD
                     LFPGANKALTEEGFLHVFPQIYDCEGFFVARLRKTQAIPVLPAPKYKVGNFPFSPVKD
                     REAGQIRQAAAGVGLNWDGNLRLWQRDKELWLFPVGIEALIGKVRFSRLGIKLAETHN
                     KGYRWQHEAVIALASPDNVNAFELTPQEAEEWYRGRDVYPQAAPVADDVLVTFQHQPI
                     GLAKRIGSRLKNSYPRELVRDGKLFTGNA"
     gene            complement(5755..8388)
                     /gene="yebT"
                     /locus_tag="PN977_004088"
     CDS             complement(5755..8388)
                     /gene="yebT"
                     /locus_tag="PN977_004088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-binding membrane homeostasis protein YebT"
                     /protein_id="HDI6044066.1"
                     /translation="MSQETPASTTEAQIKNKRRISPFWLLPFIALMIAGWLIWDSYQD
                     RGNTVTIDFMSADGIVPGRTPVRYQGVEVGTVQDISLSDDLRKIEVKVSIKSDMKDAL
                     REETQFWLVTPKASLAGVSGLDALVGGNYIGMMPGKGKEQDHFVALDTQPKYRLDNGD
                     LMIHLQAPDLGSLSSGSLVYFRKIPVGKVYDYAINPNKQGVVIDVLIERRFTDLVKKG
                     SRFWNVSGVDANVSISGAKVKLESLAALVNGAIAFDSPEESKPAEAEDTFGLYEDLAH
                     SQRGVIIKLELPSGAGLTADSTPLMYQGLEVGQLTKLDLNPGGKVTGEMTVDPSVVTL
                     LRENTRIELRNPKLSLSDANLSALLTGKTFELVPGDGEPRKEFVVVPGEKALLHEPDV
                     LTLTLTAPESYGIDAGQPLILHGVQVGQVIDRKLTSKGVTFTVAIEPQHRELVKGDSK
                     FVVNSRVDVKVGLDGVEFLGASASEWINGGIRILPGDKGEMKASYPLYANLEKALENS
                     LSDLPTTTLSLSAETLPDVQAGSVVLYRKFEVGEVITVRPRANAFDIDLHIKPEYRNL
                     LTSNSVFWAEGGAKVQLNGSGLTVQASPLSRALKGAISFDNLSGASASQRKGDKRILY
                     ASETAARAVGGQITLHAFDAGKLAVGMPIRYLGIDIGQIQTLYLITARNEVQAKAVLY
                     PEYVQTFARGGTRFSVVTPQISAAGVEHLDTILQPYINVEPGRGNPRRDFELQEATIT
                     DSRYLDGLSIIVEAPEAGSLGIGTPVLFRGLEVGTVTGMTLGTLSDRVMIAMRISKRY
                     QHLVRNNSVFWLASGYSLDFGLTGGVVKTGTFNQFIRGGIAFATPPGTPLAPKAQEGK
                     HFLLQESEPKEWREWGTALPK"
     gene            complement(8357..9640)
                     /gene="yebS"
                     /locus_tag="PN977_004089"
     CDS             complement(8357..9640)
                     /gene="yebS"
                     /locus_tag="PN977_004089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity lipid transport subunit YebS"
                     /protein_id="HDI6044067.1"
                     /translation="MALNTPQITPTKKITVRAIGEELPRGDYQRCPQCDMLFSLPEIN
                     SHQSAYCPRCQAKIRDGRDWSLTRLAAMAFAMLLLMPFAWGEPLLHIWLLGIRIDANV
                     MQGIWQMTKQGDAITGSMVFFCVIGAPLILVTSIAYLWFGNRLGMNLRPVLLMLERLK
                     EWVMLDIYLVGIGVASIKVQDYAHIQAGVGLFSFVALVILTTVTLSHLNVEELWERFY
                     PQCPATRRDEKLRVCLGCHFTGYPDQRGRCPRCHIPLRLRRRHSLQKCWAALLASIVL
                     LLPANLLPISIIYLNGGRQEDTILSGIMSLASSNIAVAGIVFIASILVPFTKVIVMFT
                     LLLSIHFKCQQGLRTRILLLRMVTWIGRWSMLDLFVISLTMSLINRDQILAFTMGPAA
                     FYFGAAVILTILAVEWLDSRLLWDAHESGNARFDD"
     gene            9770..10267
                     /gene="msrC"
                     /locus_tag="PN977_004090"
     CDS             9770..10267
                     /gene="msrC"
                     /locus_tag="PN977_004090"
                     /EC_number="1.8.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine (R)-S-oxide reductase"
                     /protein_id="HDI6044068.1"
                     /translation="MNKAEFYADFNRDFNALMAGETSFLATLANTSALLYERLTDVNW
                     AGFYLLDDDTLVLGPFQGKIACVRIPVGRGVCGTAVARNQVQRIEDVHAFDGHIACDA
                     ASNSEIVLPLVVKNQIIGVLDIDSTVFGRFTDEDEQGLRQLVAQLEKVLATTDYKKFF
                     ASVAG"
     gene            10364..11062
                     /gene="proQ"
                     /locus_tag="PN977_004091"
     CDS             10364..11062
                     /gene="proQ"
                     /locus_tag="PN977_004091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707288.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone ProQ"
                     /protein_id="HDI6044069.1"
                     /translation="MENQPKLNSSKEVIAFLAERFPHCFSAEGEARPLKIGIFQDLVD
                     RVAGEMNLSKTQLRSALRLYTSSWRYLYGVKPGATRVDLDGNPCGELDEQHVEHARKQ
                     LEEAKARVQAQRAEQQAKKREAAAAAGEKEDAPRRERKPRPTTPRRKEGAERKPRAQK
                     PVEKAPKTVKAPREEQHTPVSDISALTVGQALKVKAGQNAMDATVLEITKDGVRVQLN
                     SGMSLIVRAEHLVF"
     gene            11082..>12197
                     /locus_tag="PN977_004092"
     CDS             11082..>12197
                     /locus_tag="PN977_004092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HDI6044070.1"
                     /translation="MNMFFRLTALAGLLAIAGQTFAVEDITRADQIPVLKEETQHATV
                     SERVTSRFTRSHYRQFDLDQAFSAKIFDRYLNLLDYSHNVLLASDVEQFAKKKTELGD
                     ELRSGKLDVFYDLYNLAQKRRFERYQYALSVLEKPMDFTGNDTYNLDRSKAPWPKNEA
                     ELNALWDSKVKFDELSLKLAGKTDKEIRETLTRRYKFAIRRLAQTNSEDVFSLAMTAF
                     AREIDPHTNYLSPRNTEQFNTEMSLSLEGIGAVLQMDDDYTVINSMVAGGPAAKSKAI
                     SVGDKIVGVGQTGKPMVDVIGWRLDDVVALIKGPKGSKVRLEILPAGKGTKTRTVTLT
                     RERIRLEDRAVKMSVKTVGKEKVGVLDIPGFYVGLTDD"
BASE COUNT         3100 a         3076 c         3089 g         2932 t
ORIGIN      
        1 tcgggttctc ccagacattg ccctatcgta aacagtgaac aggtcgggtc catatccata
       61 ccttccggtt gttcatcgac caccgtgatg gttctggaac gacggcccag acatgcacca
      121 taactctgat ggtagatccc tggcgttttt cgccacggtg cgaacaccgc aattccacga
      181 ataaagcgat cgttatattc aacgggaagg ccggcaatga tagtcattcg ccaggtcgtt
      241 gcagcataac aaagcgggtc taatagcgag agatcggggg gggcaggaag ggcacgtcgc
      301 gaataatcac accccagtaa agaaagcgat ggaaaaacta acagctgaca ttgttgcctg
      361 gcggcggccc tcacaaactc cagatgatga gctacctgtt cggtgagtga ggttttacga
      421 ggctcatatt gcgctgctgc aactttccag aacgacataa tgacttcctt ttcaaaatga
      481 ctgcaaatta tgggtgcggt ttagaaaata ctcataattt agcaggcgtt atgtaagaaa
      541 gtgtaactat gtataagttt tatttaagtt tgggctcgta aggtaaacgt gacagattga
      601 ccgaagccaa acggtgggcg ataagccgct cgcgaaacca gctgcgcaaa tgttcaggct
      661 gttcacgttc aaccgcttca gcgatcaccg gcatattgta gcgttcttta aatgcaaccc
      721 ctgccgccgc caaatcgaca ttcactttat ccatttctgt ttgatccagt ttagccagat
      781 tcttcagcat tcttaatctc ctcatcattc gcgggcaaaa gttactaaga gtgagcctgg
      841 atggcaagcc ggaattgatg atcctcgact cagacattag acaaggttat gctctgtaaa
      901 aaagttatta caaaaaggaa tagataatat ggcttcaact gcacgctccc ttcgctacgc
      961 gctggcaatc ctgaccactt cgttggtgac accttccgtc tgggcacatg cgcatttaat
     1021 gcatcagtat cctgcagcaa atgcgcaact gacagctgcc ccgcaggcaa tcactttaaa
     1081 cttctcggaa ggcgttgaaa caggattcag tggtgcaaaa atcacggggc caaaaaacga
     1141 aaatattaaa acattaccgg cgaagcgaaa tgagcaggac ccaaagcaac tgattgttcc
     1201 actggccgat tcgctgaaac ccggaaccta caccgttaac tggcatgttg tttcggtgga
     1261 cgggcataaa acgaaaggac actacacctt tagcgtgaaa taaatcatgc tggcgtttac
     1321 ctggatcgcg ctgcgattta tccatttcac ctcgctgatg ttggtttttg gcttcgcaat
     1381 gtacggtgcc tggctggccc ccttaacgat tcgtcgcttg ctgacgaagc gttttctgcg
     1441 tttacaacag cacgctgccg tctggagttt gatcagtgcc actgcaatgc tcgccgttca
     1501 gggcgggttg atgggaacgg gatggtcaga tgtattttcg ccaaacattt ggcaggctgt
     1561 tttgcaagcg cagtttggtg gcgtctggtt atggcaaatt gttctcgccc tcgtaacgtt
     1621 gatcgtcgcc cttatgcaac cgcggaatat gccacgtttg ctgtttatgc tcactaccgc
     1681 gcaatttttt ctgctggcgg gagtcgggca tgcgacgctg aatgaagggg taacggcgaa
     1741 aatccatcag actaatcacg cgattcacct gatttgtgcg gcggcgtggt ttgggggatt
     1801 actgccggtg ctctggtgta tgcaactcat caaaggccgt tggcgacacc aggctattca
     1861 ggcgctgatg cgtttttcct ggtgcgggca ttttgcggtg atcggcgtac tggcaagcgg
     1921 cgtgcttaat gcattgttga ttaccggatt tccccccacg cttacgactt actggggcca
     1981 actcttattg ctcaaagcca tcctggtgat gattatggtg gtcattgcgc tggctaatcg
     2041 ttatgttctt gtaccgcgca tgaggcagga tgaagatcgt gcggcaccct ggtttgtgtg
     2101 gatgactaaa ctcgaatggg cgataggtgc ggtagtgctg gtgatcatca gcctgcttgc
     2161 gactctcgaa cctttttgat ggattacgaa aatgatgaaa aaaagtattc tggcgtttct
     2221 gttactcacc agttctgcag cggcgctggc tgcaccgcag gtgattaccg tcagccgttt
     2281 tgaagtgggt aaagacaaat gggcgtttaa tcgcgaagag gtgatgctga cttgccgacc
     2341 gggtaatgct ttgtatgtca tcaacccaag taccctcgtg cagtatcctt taaacgatat
     2401 cgcacaaaag gaagttgcca gtgggaagac taaagcccaa cccatttcgg tgattcagat
     2461 taatgatcct aacaatcccg gcgaaaaaat gagtctggca ccgtttatag aacgagctga
     2521 aaaactctgt taattaccta aaatagcctt ttgatttcca ataaaaaaac cgcctcagtt
     2581 ctttcaccag aacgggcggt ttttaacatt taagctgatg accaccgcgc tttttattga
     2641 ccattttgca cgcaaactgg aaaatctggc gtcgtcatct attcttaaag agcaaggcaa
     2701 ctaagcctgc attaatgcca acttttagcg cacggctctc tcccaagagc catttccctg
     2761 gaccgaatac aggaatcgtg ttcggtctct ttttatctgt taaaagccag aagcattccc
     2821 ttcgctgact ttatagtcaa ccataacaca cactctactg tctgagtcca gcgtttttta
     2881 acattcttgt taagattatg tgatctttag cgcgggagga aaatattgat gaaacagcct
     2941 gcgcccgttt atcagagaat tgcgggtcat caatggcgac atatctggct ttcaggcgat
     3001 atacacggtt gtcttgagca gttgcgccgc aaattatggc attgtcgttt tgatccgtgg
     3061 cgagatttac ttatctcagt gggagacgtt atcgatcgtg ggcagcaaag tttacgttgt
     3121 ctgcagttac tggaacaaca ttgggttcgt gcggtaagag gcaatcatga acagatggcg
     3181 atggatgcgc tggcatccca gcagatgtct ttgtggttga tgaatggcgg caactggttt
     3241 attgcgctgg cagattatca acagaaacaa gcgaaaacgg cgctggaaaa atgtcagtat
     3301 ttgcccttta ttcttgaagt acacagtcgc accggcaaac atgttattgc ccatgccgat
     3361 tatccggatg atgtttatga atggcaaaag gacgtcgatt tgcatcaggt cttgtggagc
     3421 cgctcgcggt taggtgaacg ccaaaaaggg cagggaatta caggtgctga tcatttctgg
     3481 tttggtcata caccgttgcg ccatcgcgtg gatattggca acctgcatta tattgataca
     3541 ggtgctgtct ttgggggcga actgactctt gtgcaattgc aataattaaa aatcaccgta
     3601 ttcctgtgca ggtcgccaga aaccatctat aaaatcctca atcggaaaac aaccgccatg
     3661 gcggatccgt tgatcgctca tagaataaag acactgctgt tccgtgttgt agacatccac
     3721 aacaatatct tcacaaccgc catccaggta gcaaacaaaa agtaccagcg cgaacatttc
     3781 atccccgaag tgtggtgccg taccgttaag tttaggtgag attttacaac gggggaataa
     3841 ccaggacaaa taacccgcca tgccgacggg ttctttttgg atcaggcaag acgcataatc
     3901 caggcatcag attgttgatc gtaacgggta cggtaaagga cagagtgttc gccatttagc
     3961 agggcaggga tactggtttc agcatcccag gcatccagtt gcatacataa atcggggtct
     4021 gatttacaag gaatggttaa cgttcgatca cccggcgatt cgccgtgtaa ttcgccgtcg
     4081 tcgatttcaa aagcgcctat gcgcacactg gttttcatct tcgtgctctc catttagctg
     4141 ccgattgtgg tacgaccagt ccgatcgcac catttttcag cctagttgag gcagccgaat
     4201 gtgccagtca aaaagtgcgc tttttttgtg cgctgtcagg cgttaccggt aaaaagtttc
     4261 ccgtctcgca ccagttcacg cgggtagctg tttttcagtc gcgaaccaat tcgtttggct
     4321 aaaccaatcg gctgatgctg gaaagtaacc aatacatcat ccgctactgg cgcggcttgc
     4381 gggtaaacat cgcgcccgcg ataccactct tccgcttcct gcggtgtcag ttcaaaagcg
     4441 ttcacattgt cgggggaggc aagggcaata accgcttcat gctgccagcg ataacctttg
     4501 ttatgcgttt cggcaagttt aatccccaac cgagaaaatc ggactttacc gatcagggct
     4561 tcaatgccta ccgggaacaa ccacagttct ttgtcgcgtt gccagagtcg caggtttcca
     4621 tcccagttta agccaacacc tgcagccgcc tgacgaattt gaccggcttc gcgatctttc
     4681 accgggctaa acgggaaatt gcccactttg tattttgggg cgggtaagac ggggatcgcc
     4741 tgagttttac gcagacgagc aacgaagaag ccttcgcagt cgtaaatttg tgggaaaaca
     4801 tgcaaaaagc cttcttcggt cagcgcctta tttgcaccag ggaagagatc gccaagcggt
     4861 aaaaactcta ctgcgtcggg gtaagtctct ttcagccaca ggcaaacggc ttcgttttct
     4921 tccctgttta aggtacaggt cgagtaaacc agcgtaccgc cagggcgtaa tgcatgaaag
     4981 gcgctgtcga tcagttcccg ttgggtcgct gcgatttcct gattgctttc tggtgaccag
     5041 ttttttagcg catcgggatc tttacgcacc acgccttcgc cggagcaggg agcgtccagc
     5101 aaaatggcat cgaacatttc tggcactgcc gcaccaaaca cgcggccatc aaaatgtgtg
     5161 agcgcaacat tactgatgcc acagcggctg atattggcat gtaacacttt tacccgactg
     5221 gcggaaaact cattggcaag gatcgcccct tcgttattca tccgcgcggc aatttgcgtc
     5281 gttttggagc cgggcgcggc agcgacatcc atcacccgct gtggtgcatt accgtcagca
     5341 aacaaggcgg caacgggcaa cattgaactg gcttcctgaa tataaaacag gccgcttaaa
     5401 tgctcggcgg tactacccaa tggcaatgca tcttcatcgt cgcgttcaat ccagaaacct
     5461 tcttcacacc acggaattgg cgtaagcgtc cagccataag gagcggttaa ttgcaggaaa
     5521 tcagcaacgg agattttcag cgtattaacg cgaatgctgc ggcgcaacgg gcgctgacag
     5581 gcggcaagaa aatcatcaaa agagagcgtc gaaggcatag cttcgcgcat ttgtgtcaga
     5641 aaggcgtccg ggaaataaac ggtgtgttgg gccacgagca tataccactg gcaaaaaaac
     5701 aggcgcgcag tttagcataa acgctccggc gcagacacgc cggagcagtg ggcattattt
     5761 gggaagcgca gttccccatt cacgccactc tttcggttca ctttcctgca acaggaagtg
     5821 cttgccttcc tgggctttcg gtgccagtgg cgtacccgga ggcgtggcga aggcgatgcc
     5881 gccacggata aattggttaa aggtgccggt tttcactact ccgcccgtca gaccaaagtc
     5941 cagactgtaa cctgatgcca accagaagac ggaattgttg cgcaccaggt gttgatagcg
     6001 tttactgatg cgcatcgcaa tcatcacgcg atcagacaat gtccccagcg tcattccggt
     6061 aaccgtaccg acttccagac cacggaacag cacaggcgta ccaataccta acgaaccggc
     6121 ttccggcgct tcaacaataa tgcttaagcc atccaggtaa cgcgaatcag taatggtggc
     6181 ttcttgcaac tcaaagtcgc ggcgaggatt accccgacct ggttcgacgt tgatatacgg
     6241 ctggaggatg gtatcaagat gctcaacgcc cgcggctgaa atttgcggtg tgaccaccga
     6301 gaagcgcgta ccgccgcggg cgaaggtctg gacatactcc ggatagagca ccgcttttgc
     6361 ctgcacttca ttgcgcgcgg taatcagata cagcgtctgg atttgcccga tatcaatacc
     6421 gagatagcga attggcatcc cgaccgccag ttttccggca tcgaaagcgt gaagcgtaat
     6481 ctgcccgcca accgcacggg ccgctgtttc ggaagcatac agaatacgtt tgtcgccttt
     6541 acgctgactg gcgctggcac cgctgaggtt atcgaagcta atggctccct ttaatgctct
     6601 ggagagcggg gatgcctgta cggtcaggcc actaccattc agctgaactt tcgccccgcc
     6661 ttctgcccag aacacgctat tgctggtcag aaggttgcga tactccggct taatatgcag
     6721 atcgatatca aacgcgttag ctcgcggacg cacggtaata acttcaccaa cttcaaattt
     6781 acggtacagc actaccgatc ctgcctgcac atccggcagc gtctctgcac tcaaacttag
     6841 ggttgtggtg ggtaaatcgc taaggctgtt ctccagcgct ttttccagat tggcatacag
     6901 tggatagctg gctttcatct cgcctttatc gcccggcaga atacgtatcc cgccgttaat
     6961 ccattctgag gcgctggctc cgagaaactc aacgccatcc agccccacct tcacgtcgac
     7021 acggctgttg acgacaaatt tgctatcgcc ttttaccagt tctcgatgct gaggctcgat
     7081 ggcgacggta aaggtgacgc ctttgctggt gagtttacga tcaataacct ggcctacctg
     7141 cacgccgtga agaatgagcg gctgaccagc atcaataccg taactttccg gtgctgtcag
     7201 ggtcagtgtc agaacatcag gttcatgcag cagtgctttt tcgcctggca caacaacgaa
     7261 ctctttgcgt ggctcgccat cgccgggcac caactcaaag gtttttccgg tcagcagggc
     7321 gctgagattg gcatcgctaa gggataattt cgggttgcgt aattcgatgc gggtattttc
     7381 acgaagcagg gtgacgacgc tgggatcaac ggtcatttcc ccagtgactt taccaccagg
     7441 atttaaatcc agtttagtca gctgtccgac ttccagcccc tgatacatta acggcgtcga
     7501 gtcggcggtt aatccggccc cactcggcag ttccagtttt attattacgc cacgctggct
     7561 gtgggcgaga tcttcataca gaccaaaggt atcttccgcc tcggcaggtt tcgactcttc
     7621 aggtgaatcg aaggcaatcg caccgttaac cagtgccgcc agactttcca gtttcacctt
     7681 cgcgccactg atactgacgt tggcatcaac gccggaaacg ttccagaaac ggctaccttt
     7741 tttcaccaga tcggtaaaac gccgctcgat caggacatca atcaccacgc cttgcttatt
     7801 gggattgatg gcatagtcgt agactttacc caccgggatc ttgcggaaat agaccaatga
     7861 accactgctc agtgaaccga gatcgggggc ttgcaggtgg atcatcagat cgccattgtc
     7921 cagccgatat ttcggttggg tgtcgagtgc gacaaagtga tcctgctctt tacctttgcc
     7981 cggcatcatg ccgatataat taccaccgac gagggcgtcc agcccggaga cacctgccaa
     8041 cgaggccttt ggcgtcacca accagaactg tgtctcttcg cgcagcgcat ctttcatatc
     8101 ggacttgatg ctgaccttga cttcaatctt acgaagatcg tcgctgaggc tgatatcctg
     8161 cacagttccg acttcaacgc cctgataacg aacaggcgta cggcctggaa caataccatc
     8221 cgccgacata aagtcgatgg tgacagtatt accccggtcc tgataactgt cccaaatcag
     8281 ccaaccagca atcattagcg cgatgaacgg cagcagccag aaaggtgaga tacggcgttt
     8341 atttttaatc tgcgcttcag tcgtcgaagc gggcgtttcc tgactcatgt gcatcccaaa
     8401 gtaagcggct gtccagccat tccacagcaa gaatagtcaa aattaccgct gcgccgaaat
     8461 aaaacgcagc cggtcccata gtaaaagcga ggatctgatc gcgattaatc agcgacatgg
     8521 ttaaagatat gacaaacagg tcgagcatcg accagcgacc aatccaggtc accatccgca
     8581 gtaacagaat gcgtgtgcgt aaaccttgct ggcatttaaa atgaatactc aacagtaaag
     8641 tgaacatgac gatcacttta gtaaacggta ccagaatact ggcgataaac acgattcccg
     8701 caaccgcaat gttgctactt gccagcgaca taattccgga aagaatcgta tcttcctgcc
     8761 gaccaccatt cagataaatg atagagatag gcaacaggtt ggcaggtaac aacaaaacga
     8821 ttgacgctaa cagcgccgcc cagcattttt gcagactatg acggcgacgc aggcgtagcg
     8881 ggatatggca acgcgggcag cgaccacgct gatcaggata gccggtaaaa tgacacccaa
     8941 gacagacacg gagtttctcg tcccttcgcg tagcggggca ctgcggataa aatcgctccc
     9001 acagttcttc gacattaaga tgtgacaacg tcaccgtcgt taaaatcacc aacgccacaa
     9061 aagagaacaa gccgacaccc gcctggatat gggcataatc ctgtaccttt atagaagcaa
     9121 cgccaatgcc gaccaggtag atatcgagca ttacccactc tttcagtcgc tcaagcatca
     9181 gcagtaccgg acgtaaattc attcccagtc ggttaccaaa ccataaataa gctatggagg
     9241 tcaccagaat aaggggggca ccaataacgc aaaaaaagac catcgacccc gttatcgcat
     9301 cgccctgttt ggtcatttgc cagatgcctt gcataacgtt ggcgtcaata cggatgccta
     9361 acagccagat atgcaacagc ggttcgcccc aggcaaacgg catcaacaac agcatagcga
     9421 acgccattgc cgccaggcgc gttagcgacc agtcgcgccc gtcacgaatt tttgcctgac
     9481 agcgcggaca ataggcactt tgatgagaat ttatctcggg caggctaaac agcatgtcac
     9541 attgcgggca acgttggtaa tcaccacgcg gcagttcctc gccgatagcc ctcactgtta
     9601 tcttctttgt cggcgtaatt tgtggtgtgt taagagccat gacgtgccaa atgtaataag
     9661 aattaacatt aatcttaacc catgatggga atgatcttga gcattaacgc atttaatgct
     9721 tattttaata ggctggcagt tccaggtaag acaaccaagt gattatataa tgaacaaagc
     9781 agaattttac gcggatttta atcgcgactt taacgcgctg atggcgggag aaaccagttt
     9841 tctggcaacg cttgcgaaca ccagtgcgtt gttatatgag cgtctcactg acgtaaactg
     9901 ggcaggtttt tatttgcttg acgacgatac actggtgctc ggaccatttc agggcaaaat
     9961 tgcctgtgtc cggatacccg ttgggcgcgg cgtatgcggc actgcggttg cccgcaatca
    10021 agtgcagcgt atcgaggatg ttcacgcgtt tgacgggcat attgcctgtg atgcggcgag
    10081 taattctgaa attgttctgc cactggtggt gaaaaatcag attattggtg ttctcgacat
    10141 cgacagtacc gtcttcggtc gctttacaga cgaggacgag caaggcttac gtcagcttgt
    10201 ggcacagctt gaaaaggtgc ttgcaacgac ggattacaaa aaattctttg cgagcgtcgc
    10261 aggataatca acggataacg tagcatttac tgatagcgtc attataatga cgcctgttca
    10321 tgcctgcgct tgttggctac gtccgttgta atcaggaaat ttcatggaaa atcaacctaa
    10381 gttgaatagc agtaaagaag taatcgcgtt tctggccgaa cgttttcccc actgtttcag
    10441 tgcggaaggt gaagcgcgtc cgctgaaaat cggtattttt caggatttgg tcgatcgtgt
    10501 tgccggggaa atgaacctga gcaaaacgca attgcgatcc gctttacgtc tctacacttc
    10561 gagctggcgt tacctttacg gtgttaaacc cggcgcaacg cgtgtcgatc ttgacggcaa
    10621 cccatgcggt gagctggacg agcaacatgt cgagcatgct cgcaagcagc ttgaagaagc
    10681 gaaagcgcgt gttcaggcac agcgtgctga acagcaagcg aaaaaacgcg aagctgccgc
    10741 agctgctggt gagaaagaag acgcaccgcg ccgcgaacgc aagccacgtc cgaccacgcc
    10801 acgccgcaaa gaaggcgctg aacgtaaacc tcgtgcgcaa aagccggtag agaaagcgcc
    10861 aaaaacagta aaagcacctc gcgaagaaca gcacaccccg gtttctgaca tttcagctct
    10921 gactgtcgga caagccctga aggtgaaagc gggtcaaaac gcgatggatg ccaccgtatt
    10981 agaaatcacc aaagacggcg tccgcgtcca gctgaattcg ggtatgtctt tgattgtgcg
    11041 cgcagaacac ctggtgttct gaaacggagg ccgggccagg catgaacatg ttttttaggc
    11101 ttaccgcgtt agctggcctg cttgcaatag caggccagac cttcgctgta gaagatatca
    11161 cgcgtgctga tcaaattccg gtattaaagg aagagacgca gcatgcgacg gtaagtgagc
    11221 gcgtaacgtc gcgcttcacc cgttctcatt atcgccagtt cgacctcgat caggcatttt
    11281 cggccaaaat ctttgaccgc tacctgaatc tgctcgatta cagccacaac gtgctgttgg
    11341 caagcgatgt tgaacagttc gcgaaaaaga aaaccgagtt aggcgatgaa ctgcgttcag
    11401 gcaaactcga cgttttctac gatctctaca atctggcgca aaagcgtcgt tttgaacgtt
    11461 accagtacgc tttgtcggta ctggaaaagc cgatggattt caccggcaac gacacttata
    11521 accttgaccg cagcaaagcg ccctggccga aaaacgaggc tgagttgaac gcgctgtggg
    11581 acagtaaagt caaattcgac gagttaagcc tgaagctggc aggaaaaacg gataaagaaa
    11641 ttcgtgaaac cctgactcgc cgctacaaat ttgccattcg tcgtctggcg caaaccaaca
    11701 gcgaagatgt tttctcgctg gcaatgacgg cgtttgcgcg tgaaatcgac ccgcatacca
    11761 actatctttc cccgcgaaat accgaacagt tcaacactga aatgagtttg tcgctggaag
    11821 gtattggcgc agtgctgcaa atggatgatg actacaccgt tatcaattcg atggtggcag
    11881 gtggcccggc agcgaagagt aaagctatca gcgttggtga caaaattgtc ggtgttggtc
    11941 aaacaggcaa gccgatggtt gacgtgattg gttggcgtct tgatgatgtg gttgccttaa
    12001 ttaaagggcc gaagggcagt aaagttcgtc tggaaatttt acctgctggt aaagggacca
    12061 agacccgtac tgtaacgttg acccgtgaac gtattcgtct cgaagaccgc gcggttaaaa
    12121 tgtcggtgaa gaccgtcggt aaagagaaag tcggcgtgct ggatattccg ggcttctatg
    12181 tgggtttgac agatgat
//
LOCUS       DAMZFT010000140        12113 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.140, whole genome shotgun sequence.
ACCESSION   DAMZFT010000140 DAMZFT010000000
VERSION     DAMZFT010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12113)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12113)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12113
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.140"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..404
                     /locus_tag="PN977_004093"
     CDS             <1..404
                     /locus_tag="PN977_004093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HDI6044071.1"
                     /translation="TVKITQQNRLNAKSSSGVYLLPGAKTPARLESQIGTLRMSLVNI
                     THDTDGTTLTLRIQGESNDPLPAFSGTVEYGQIQGTIDNFQEINLQNQLINAPASVLV
                     PSDVDIPLQLKGISVEQLDFVRIHDIQPVMQ"
     gene            703..1587
                     /gene="tsx"
                     /locus_tag="PN977_004094"
     CDS             703..1587
                     /gene="tsx"
                     /locus_tag="PN977_004094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-specific channel-forming protein Tsx"
                     /protein_id="HDI6044072.1"
                     /translation="MKKTLLAAGAVLALSSSFTVNAAENDKPQYLSDWWHQSVNVVGS
                     YHTRFGPQIRNDTYLEYEAFAKKDWFDFYGYADAPVFFGGNSDAKGIWNHGSPLFMEI
                     EPRFSIDKLTNTDLSFGPFKEWYFANNYIYDMGRNKDGRQSTWYMGLGTDIDTGLPMS
                     LSMNVYAKYQWQNYGAANENEWDGYRFKIKYFVPITDLWGGQLSYIGFTNFDWGSDLG
                     DDSGNAINGIKTRTNNSIASSHILALNYDHWHYSVVARYWHDGGQWNDDAELNFGNGN
                     FNVRSTGWGGYLVVGYNF"
     gene            complement(1625..1972)
                     /locus_tag="PN977_004095"
     CDS             complement(1625..1972)
                     /locus_tag="PN977_004095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH nuclease family protein"
                     /protein_id="HDI6044073.1"
                     /translation="MAIIPKNYARLESGYREKALKIYPWVCGRCSREFVYSNLRELTV
                     HHIDHDHTNNPEDGSNWELLCLYCHDHEHSKYTEADQYGTTVIAGEDAQKDVGEAKYN
                     PFADLKAMMNKKK"
     gene            complement(2060..2341)
                     /locus_tag="PN977_004096"
     CDS             complement(2060..2341)
                     /locus_tag="PN977_004096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HDI6044074.1"
                     /translation="MYKLSGKAVEDFRGIYDYTLGKFGEEQADRYTDSLGIFLDTLSQ
                     MPEIGQDYDAIPEVKKIAFRFHTVYYVIRVDDILIARILHQLMEPRRHW"
     gene            complement(2334..2576)
                     /locus_tag="PN977_004097"
     CDS             complement(2334..2576)
                     /locus_tag="PN977_004097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system ParD family
                     antitoxin"
                     /protein_id="HDI6044075.1"
                     /translation="MRKITSVSVGEQLDSFITRMVQSGRYGSASEVMRSALRLLEQQE
                     SRDEAVRNAVIEGLESGESSMTLRDIAAERKQKHRV"
     gene            complement(2727..3698)
                     /gene="secF"
                     /locus_tag="PN977_004098"
     CDS             complement(2727..3698)
                     /gene="secF"
                     /locus_tag="PN977_004098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HDI6044076.1"
                     /translation="MAQEYTVEQLNHGRKVYDFMRWDYWAFGISGLLLIAAIVIMGVR
                     GFNWGLDFTGGTVIEITLEKPAEIDVMRDALQKAGFEEPMLQNFGSSHDIMVRMPPAE
                     GETGGQVLGSQVLKVINESTNQNAAVKRIEFVGPSVGADLAQTGAMALMAALLSILVY
                     VGFRFEWRLAAGVVIALAHDVIITLGILSLFHIEIDLTIVASLMSVIGYSLNDSIVVS
                     DRIRENFRKIRRGTPYEIFNVSLTQTLHRTLITSGTTLMVILMLYLFGGPVLEGFSLT
                     MLIGVSIGTASSIYVASALALKLGMKREHMLQQKVEKEGADQPSILP"
     gene            complement(3709..5556)
                     /gene="secD"
                     /locus_tag="PN977_004099"
     CDS             complement(3709..5556)
                     /gene="secD"
                     /locus_tag="PN977_004099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HDI6044077.1"
                     /translation="MLNRYPLWKYVMLIVVIVIGLLYALPNLFGEDPAVQITGARGVA
                     ASEQTLIQVQKTLQEEKITAKSVALEEGAILARFDSTDTQLRAREALMGVMGDKYVVA
                     LNLAPATPRWLAAIHAEPMKLGLDLRGGVHFLMEVDMDTALGKLQEQNIDSLRSDLRE
                     KGIPYTTVRKENNYGLSITFRDAKARDEAIAYLSKRHPDLVISSQGSNQLRAVMSDAR
                     LSEAREYAVQQNINILRNRVNQLGVAEPVVQRQGADRIVVELPGIQDTARAKEILGAT
                     ATLEFRLVNTNVDQAAAASGRVPGDSEVKQTREGQPVVLYKRVILTGDHITDSTSSQD
                     EYNQPQVNISLDSAGGNIMSNFTKDNIGKPMATLFVEYKDSGKKDANGRAVLVKQEEV
                     INIANIQSRLGNSFRITGINNPNEARQLSLLLRAGALIAPIQIVEERTIGPTLGMQNI
                     EQGLEACLAGLLVSILFMIIFYKKFGLIATSALIANLILIVGIMSLLPGATLSMPGIA
                     GIVLTLAVAVDANVLINERIKEELSNGRTVQQAIDEGYRGAFSSIFDANITTLIKVII
                     LYAVGTGAIKGFAITTGIGVATSMFTAIVGTRAIVNLLYGGKRVKKLSI"
     gene            complement(5584..5916)
                     /gene="yajC"
                     /locus_tag="PN977_004100"
     CDS             complement(5584..5916)
                     /gene="yajC"
                     /locus_tag="PN977_004100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HDI6044078.1"
                     /translation="MSFFISDAVAATGAPAQGSPMSLILMLVVFGLIFYFMILRPQQK
                     RTKEHKKLMDSIAKGDEVLTNGGLVGRVTKVAENGYIAIALNDTTEVVIKRDFVAAVL
                     PKGTMKAL"
     gene            complement(5939..7066)
                     /gene="tgt"
                     /locus_tag="PN977_004101"
     CDS             complement(5939..7066)
                     /gene="tgt"
                     /locus_tag="PN977_004101"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HDI6044079.1"
                     /translation="MKFELDTTDGRARRGRLVFDRGVVETPCFMPVGTYGTVKGMTPE
                     EVEATGAQIILGNTFHLWLRPGQEIMKLHGDLHDFMQWKGPILTDSGGFQVFSLGDIR
                     KITEQGVHFRNPINGDPIFLDPEKSMEIQYDLGSDIVMIFDECTPYPADWDYAKRSME
                     MSLRWAKRSRERFDSLGNKNALFGIIQGSVYEDLRDISVKGLVDIGFDGYAVGGLAVG
                     EPKADMHRILEHVCPQIPADKPRYLMGVGKPEDLVEGVRRGIDMFDCVMPTRNARNGH
                     LFVTDGVVKIRNAKYKSDTGPLDPECDCYTCRNYSRAYLHHLDRCNEILGARLNTIHN
                     LRYYQRLMAGLRKAIEEGKLESFVTDFYQRQGREVPPLNVD"
     gene            complement(7121..8191)
                     /gene="queA"
                     /locus_tag="PN977_004102"
     CDS             complement(7121..8191)
                     /gene="queA"
                     /locus_tag="PN977_004102"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HDI6044080.1"
                     /translation="MRVTDFSFELPESLIAHYPMPERSSCRLLSLDGPTGALTHGTFT
                     DLLDKLNPGDLLVFNNTRVIPARLFGRKASGGKIEVLVERMLDDKRILAHIRASKAPK
                     PGAELLLGDDESINATMTARHGALFEVEFNDDRSVLDILNSIGHMPLPPYIDRPDEDA
                     DRELYQTVYSEKPGAVAAPTAGLHFDEPLLEKLRAKGVEMAFVTLHVGAGTFQPVRVD
                     TIEDHIMHSEYAEVPQDVVDAVLAAKARGNRVIAVGTTSVRSLESAAQAAKNDLIEPF
                     FDDTQIFIYPGFQYKVVDALVTNFHLPESTLIMLVSAFAGYQHTMNAYKAAVEEKYRF
                     FSYGDAMFITYNPQAINERVGE"
     gene            8284..8865
                     /gene="acpH"
                     /locus_tag="PN977_004103"
     CDS             8284..8865
                     /gene="acpH"
                     /locus_tag="PN977_004103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP phosphodiesterase"
                     /protein_id="HDI6044081.1"
                     /translation="MNFLAHLHLAHLAESSLSGNLLADFVRGNPEESFPPDVVAGIHM
                     HRRIDVLTDNLPEVREAREWFRSETRRVAPITLDVMWDHFLSRHWSQLSPDFPLQEFV
                     CYAREQVMTILPDSPPRFINLNNYLWSEQWLVRYRDMDFIQNVLNGMASRRPRLDALR
                     DSWYDLDAHYDALETRFWQFYPRMMAQASRKAL"
     gene            complement(8886..10331)
                     /locus_tag="PN977_004104"
     CDS             complement(8886..10331)
                     /locus_tag="PN977_004104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6044082.1"
                     /translation="MTISNSNLLKPFIPGTELPPFKEDVFSKTEEQFKKSPHGKLLFI
                     SGNENLVNISAEKIDEIKNSKDFFSNIVNIIILLIKHPDKILPILHGCPAESIELIKN
                     AKRTGQWSYEMTDKNGQNIEVSYIFTDTIDDKSDGKLCLKICNSCNQNDSSTHEYKID
                     HQQLKNHIRQLEEWALLEKFYDNNAYYSKRTKQLEKKIADAGFITGKVNADNFNVKEF
                     KDDIASMLCEQPPMRTHQISSSGYLSADKVPDLYKTNLKDAQRDLKTTSVDIDGVSLK
                     SKTREKIAEKIAKKTEEKIAKQNAKTIEEKNSEKNKKETNPEDNFVLKFSPEEINEML
                     LDYSDEEQKKLRPFFTSDHIYCLFDAFSQGLHNVARNGSPRYAGLLHQIASNSEILQD
                     FDQQLFWRTFSGGVFHRRISVNSEPEKTDVWRFSTQFDFPHINDNGELSRTVLMFEGV
                     AESNFKDIANHSSECFSYSCYAAPLDANAKK"
     gene            complement(10518..>12113)
                     /gene="malZ"
                     /locus_tag="PN977_004105"
     CDS             complement(10518..>12113)
                     /gene="malZ"
                     /locus_tag="PN977_004105"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308480.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin glucosidase"
                     /protein_id="HDI6044083.1"
                     /translation="QPRRRYSFKLLWHDRQRWFTPQGFSRMPPARLEQFAVDVPDIGP
                     QWAADQIFYQIFPDRFARSLPREAEQDHVYYHHAAGQEIILRDWDEPVTAQAGGSTFY
                     GGDLDGISEKLPYLKKLGVTALYLNPVFKAPSVHKYDTEDYRHVDPQFGGDGALLRLR
                     HHTQQLGMRLVLDGVFNHSGDSHAWFDRHNRGTGGACHNPESPWRDWYSFSDDGTALD
                     WLGYASLPKLDYQSESLVNEIYRGEDSIVRHWLKAPWNMDGWRLDVVHMLGEAGGARN
                     NLQHVAGITEAAKETQPEAYIVGEHFGDARQWLQADVEDAAMNYRGFTFPLWGFLANT
                     DISYDPQQIDAQTCMAWMDNYRAGLSHQQQLRMFNQLDSHDTARFKTLLGRDIARLPL
                     AVVWLFTWPGVPCIYYGDEVGLDGKNDPFCRKPFPWQVEKQDTALFALYQRMIALRKK
                     SQALRRGGCQVLYAEDNVVVFGRVLNQQRVLVAINRGEACEVVLPASPLLNVAQWQRK
                     EGHGQLTDGILALPAISATVWIN"
BASE COUNT         3042 a         3167 c         2815 g         3089 t
ORIGIN      
        1 aaaccgtcaa aatcactcag caaaacaggc tgaatgcaaa atccagcagt ggggtttacc
       61 ttctgcctgg agcgaaaaca ccggcaagac tggaaagcca gatcggtact ttacgtatgt
      121 cgctagtgaa tattacgcat gatacagatg gcaccaccct gacgctacgt attcagggcg
      181 agtcgaatga ccccttgccc gcattcagcg gaactgttga atatgggcaa attcagggaa
      241 caatagacaa ctttcaggaa atcaatttgc agaatcaatt gattaatgcc cctgccagcg
      301 tcctcgtccc cagcgatgtt gatattccgt tacagttaaa ggggatttct gtagaacagt
      361 tagactttgt acgtatccac gacattcaac ctgttatgca gtaaacgtat agcggggcga
      421 ttttgtgcgt cccgcaacat cttttcccgt cattttgtta ctccgcttac atcacctgga
      481 ttgatagtaa aagtttgcaa caagggtgaa agtcagtaca atccccgccc gaatgtgtgt
      541 aaacgtgaac gcaatcgatt acgtaaatga tagaactgtg aaacgaaaca tatttttgtg
      601 agcaatgatt tttataatag gctcctctgt atacgaaata tttagaaacg caatttgcgc
      661 ctttttcact cccgcaaggg attttcaaac agtggcatac atatgaaaaa aacattactg
      721 gcagccggtg cggtactggc gctctcttcg tcttttactg tcaacgcagc tgaaaacgac
      781 aaaccgcagt atctttccga ctggtggcac cagagcgtta acgttgtcgg aagctatcac
      841 acccgtttcg gaccgcagat ccgcaacgat acctaccttg agtacgaagc attcgctaaa
      901 aaagactggt tcgacttcta tggttatgcg gatgcgccgg tattcttcgg cggtaactcc
      961 gatgcaaaag gtatctggaa ccacggttct ccgctgttta tggaaatcga accgcgtttc
     1021 tccatcgaca aactgaccaa tactgacctt agcttcggtc cgttcaaaga gtggtacttc
     1081 gcgaacaact acatttacga catgggtcgt aataaagatg gtcgccagag cacctggtac
     1141 atgggtctgg gtaccgacat cgatactggt ctgccgatga gcctgtccat gaacgtctat
     1201 gcgaaatacc agtggcagaa ctatggcgca gcgaacgaaa acgagtggga cggttaccgt
     1261 ttcaaaatta aatactttgt gccgattacc gatctgtggg gcggtcagct gagctacatc
     1321 ggcttcacca acttcgactg gggttccgat ttaggggatg acagcggtaa cgcaatcaac
     1381 ggtattaaga cccgtactaa taactctatc gcttccagcc atattctggc tctgaactac
     1441 gatcattggc actactctgt cgtagctcgt tactggcacg acggtggtca gtggaacgac
     1501 gatgcagaac tgaacttcgg caacggcaac ttcaacgttc gctctaccgg ctggggtggt
     1561 tacctggtag taggttacaa cttctaattg tgagaatgcc ggatttcata tccggcattt
     1621 tttatcactt cttcttgttc atcatcgctt tcaggtcagc gaatgggttg tacttcgctt
     1681 caccgacatc tttctgcgca tcttcccctg cgataacggt cgtaccatac tgatccgctt
     1741 cggtatattt cgaatgctca tgatcgtggc aatagagaca aagcaattcc cagttactgc
     1801 catcttccgg gttattggta tggtcgtgat caatgtggtg aacggtaagt tcacgcaggt
     1861 tggaataaac aaactcacgg gaacagcgac cgcagaccca cggatagatt tttaatgctt
     1921 tttcgcgata gccgctttct aaccgcgcat agttttttgg gatgatagcc atgaaaagtt
     1981 acctggatga aatgtagggt tacgcgcagt ttcccaagat gcggggattt tcgcaatcct
     2041 gagtcaagaa attctcacat taccagtgac ggcgaggttc catcagctga tgaagaatgc
     2101 gcgcaatgag aatgtcatcg actcttataa cgtaataaac agtgtgaaac ctaaatgcga
     2161 tcttctttac ctctggaatc gcatcatagt cttgccctat ctcgggcatt tgagagagtg
     2221 tatccagaaa aatgccgaga gagtcggtat agcgatccgc ctgctcctca ccaaactttc
     2281 ccagcgtgta gtcatagatc cctctgaaat cttcaacagc ttttccgcta agcttataca
     2341 cgatgttttt gcttcctttc tgctgcgata tcacgaagcg tcatcgaact ttcaccgctc
     2401 tcaagacctt caataacagc atttctgaca gcttcatccc tggattcttg ttgttccaat
     2461 agacgaagcg ctgaacgcat tacttcactg gccgagccat aacgaccaga ctggaccatc
     2521 cgtgtaataa aactatctaa ttgttcgcca acgcttactg aggtaatttt gcgcataagc
     2581 gatctccatg tattaactat taatacaaag tatatgtcag aactctttct acaaccaaac
     2641 cgtattcagt tgcatgtgaa acaggcaaaa aaaaccgcag cgcaaatacg ccacggtttt
     2701 tttaacagaa cggatttaaa acacccttaa ggcagaattg acggctgatc cgccccttct
     2761 ttttccactt tctgctgcag catgtgttcg cgcttcatac ccagtttcag agccagcgcc
     2821 gatgctacgt agatggaaga tgcagtaccg atggaaacac cgataagcat ggtcagcgag
     2881 aagccttcca gtaccggacc accgaagagg tacagcatca ggataaccat caaggtagta
     2941 ccggatgtga tcaaggtacg gtgcagcgtc tgggtcaagg acacgttaaa gatttcgtaa
     3001 ggcgtaccgc gacggatctt gcggaagttt tcacgaatac ggtccgatac cacgatactg
     3061 tcgttaagcg agtaaccgat aactgacatc aacgatgcca caatggtcag gtcaatctcg
     3121 atatggaata acgacaaaat acccagcgta atgataacgt cgtgcgccag cgcaataaca
     3181 acccctgccg ccagtcgcca ctcaaagcgg aaacctacgt acacgaggat agacagcagc
     3241 gccgccatca acgccatcgc accggtttgc gcaaggtctg cccccacgct cggcccgacg
     3301 aactcaatac gcttcactgc cgcattctga ttggtggatt cgttaatcac cttcagaacc
     3361 tggctgccca gcacctgacc gccggtttcg ccttcagcag gcggcatacg gaccatgatg
     3421 tcgtggctgc taccaaagtt ttgcagcatc ggctcttcaa aaccggcttt ttgcaatgca
     3481 tcacgcatta cgtcaatttc agccggtttt tcgagcgtaa tttcaataac cgtaccaccg
     3541 gtgaaatcca gcccccagtt aaagccgcgc acgcccataa taacgatagc ggcgattaac
     3601 agcagaccag agatgccgaa agcccagtag tcccagcgca taaagtcata gactttacgg
     3661 ccgtggttta gttgttcaac agtatattcc tgtgccacat cgcactcctc agattgacag
     3721 cttcttgacg cgcttgccgc catatagcag gtttacgatg gcacgcgtac cgacaatcgc
     3781 ggtaaacatc gacgtcgcca caccgatacc ggtagtaatc gcgaaccctt taattgcccc
     3841 ggtacccact gcgtacagga tgatgacttt aatcagcgtg gtgatgttcg catcgaagat
     3901 ggaactgaat gcgccacgat aaccttcatc aattgcctgt tgaacagtac gtccgttgct
     3961 caactcttct ttaatacgtt cgttgatcag tacgttcgcg tcgaccgcta ccgcaagggt
     4021 taagacgata cccgcgatac ctggcatact cagcgttgcg cctggcaaca gcgacataat
     4081 accgacgatc aagatcaagt tggcaatcag agcactggtc gcaatcagac caaacttctt
     4141 atagaagatg atcatgaaca gaatagaaac cagcagaccg gcaaggcaag cctccagccc
     4201 ctgctcaatg ttctgcatac ccagggtcgg gccaattgta cgttcttcaa caatctgaat
     4261 cggcgcgatc aacgcaccgg cacgcagcag cagtgacagc tgacgggctt cgttcgggtt
     4321 gttgatgccg gtgatacgga agctgttacc cagacgagac tggatgttag cgatgttaat
     4381 cacctcttcc tgtttcacca gaaccgcacg accgttagca tctttcttgc cgctgtcttt
     4441 gtactccaca aacagggtcg ccatcggttt gccgatgttg tccttggtga agttagacat
     4501 aatgttgcca cctgcgctgt cgagcgagat gttaacctgc ggctggttgt attcgtcctg
     4561 gctggaagtg gagtcggtga tatggtcacc ggtcagaatt acgcgtttgt acagcacaac
     4621 cggctgacct tcgcgggtct gtttcacttc agagtcgccc ggtacgcggc cggatgccgc
     4681 agcggcctga tcaacgttgg tgtttaccag acggaattcc agcgttgccg tcgcacccag
     4741 aatctctttc gcacgcgcgg tgtcctgaat acctggcagt tcaacaacga tacggtcagc
     4801 accctgacgc tgaaccaccg gctccgccac gccaagttgg tttacacggt tacgcaggat
     4861 attaatgttc tgctgcaccg catattcacg cgcttcactc agacgagcat cgctcattac
     4921 cgcacgcagc tggttgctgc cctggctgct aatcaccagg tccggatggc gcttgctcag
     4981 atacgcaatg gcttcatcac gagctttagc atcgcggaaa gtgatgctca ggccgtagtt
     5041 gttttcttta cgaacggtgg tatacgggat gcccttttca cgcaggtcgc tgcgcaggct
     5101 atcgatattt tgttcctgga gtttgccaag cgcggtgtcc atatccactt ccatcaggaa
     5161 gtgaacgccg ccacgcaggt caaggccgag cttcatcggc tcagcgtgaa tagctgccag
     5221 ccagcgcggc gttgccgggg caaggttaag cgccacgacg tatttgtcac ccataacgcc
     5281 cattaatgct tcacgagcgc gcagctgggt gtcagtggag tcgaagcgcg caagaatagc
     5341 gccctcttcc agtgccacag acttagcagt tattttttct tcttgtaacg ttttctggac
     5401 ctggatcagc gtttgctcac tggcggcgac tccgcgcgca ccagtgatct gaacagccgg
     5461 atcctcacca aacaggttgg gaagcgcata cagcagaccg atgacaatca ccacgatcag
     5521 catgacgtac ttccacaaag gataacggtt taacacggca attcccttag ggaaaaattt
     5581 taattacagc gccttcatgg tgcctttcgg caggacggca gctacgaagt cacgtttaat
     5641 aactacttca gtggtgtcat tcagcgcgat agcaatgtag ccgttttccg ctactttggt
     5701 tacgcgacca accaggccac cgttcgtcag aacttcatca cctttggcaa tggagtccat
     5761 cagctttttg tgttctttgg tgcgcttctg ctgtggacgc aggatcatga aatagaaaat
     5821 cagaccgaat accaccagca tcaaaatcaa agacatcggg ctaccttgcg ccggtgcacc
     5881 cgttgccgct accgcatcag aaataaaaaa gctcattaaa tttccctcat tattaatatt
     5941 aatcaacgtt caaaggtggt acttctcgcc cctgacgctg gtaaaaatca gttacgaagc
     6001 tctctaattt accctcttca atagccttgc gtaaacccgc catcaaacgc tggtagtaac
     6061 gaaggttatg aatggtgttg agtcgcgcgc ctaatatttc gttgcaacgg tcgagatgat
     6121 gcaagtaagc gcgtgaataa ttgcgacagg tgtagcaatc acactcagga tcgagtgggc
     6181 cagtatcgct cttatacttc gcattgcgga ttttcaccac gccatcggtc acgaacaaat
     6241 gaccatttcg ggcgttgcgg gttggcatta cgcagtcaaa catatcgata ccgcgacgca
     6301 cgccttcaac caggtcttct ggtttaccaa cgcccatcag gtaacgcggt ttgtctgccg
     6361 gaatttgcgg gcatacatgc tccagaatgc ggtgcatatc tgctttcggc tcacccacag
     6421 ccagaccgcc gacagcgtag ccatcaaaac cgatatctac cagaccttta acagaaatat
     6481 cacgtaaatc ttcgtaaacg ctgccctgaa tgataccaaa cagtgcattt ttgtttccga
     6541 gactgtcaaa acgctcacgg ctacgcttcg cccaacgcag agacatctcc atggagcgtt
     6601 ttgcgtaatc ccagtcagca ggatacggcg tacactcatc aaagatcatg acgatatccg
     6661 aaccaagatc gtactgaatc tccatcgact tttccgggtc gaggaaaatc ggatcgccgt
     6721 tgatcgggtt acggaagtga acgccctgtt cggtgatttt acgaatatcg ccaaggctga
     6781 agacctggaa gccgccggag tcggtaagaa tcgggccttt ccactgcata aaatcgtgca
     6841 gatcgccatg cagtttcatg atttcctggc ccgggcgcag ccacaggtgg aaggtgttgc
     6901 cgaggataat ttgcgcgcca gtggcttcaa cttcttccgg cgtcatccct tttacggtgc
     6961 cgtaagtgcc aacaggcata aaacaaggcg tttccactac gccacgatca aagaccaggc
     7021 ggccacggcg tgcgcgaccg tcggtagtgt ccagttcaaa tttcattttt tctccactac
     7081 gtcagaaaaa cagtccaacg tttaaaccag cgccgcggaa ttactcccca acgcgctcat
     7141 taattgcctg cggattgtac gtgataaaca tcgcatcacc gtaactaaaa aagcgatatt
     7201 tctcttctac cgctgcttta taggcattca tggtgtgttg gtaaccggca aaggccgaaa
     7261 ccagcataat caacgtcgat tccggcaggt ggaagttagt caccagcgca tcgaccactt
     7321 tgtactggaa gccgggatag ataaagattt gggtatcgtc aaagaacggt tcgatgagat
     7381 cgtttttcgc cgcctgggcc gcgctttcca gcgaacgtac cgaggtggtg ccaaccgcaa
     7441 tcacccgatt tccacgcgct ttcgccgcca gtaccgcgtc aaccacatcc tgcggcactt
     7501 ctgcatattc cgagtgcatg atgtggtctt caatggtgtc gacgcgcacc ggctggaagg
     7561 tgcccgcacc aacgtgcagg gtgacaaacg ccatctccac gcctttggcg cgtaattttt
     7621 ccagcaaagg ttcgtcaaaa tgcagaccag cggtcggggc tgcaaccgcg cccggttttt
     7681 cgctataaac ggtttgataa agttcgcggt cagcgtcttc gtccggacgg tcgatatacg
     7741 gcggcagcgg catatggccg atgctgttga ggatatccag taccgaacga tcatcattaa
     7801 attcgacttc aaacagtgcg ccgtggcgcg cggtcattgt tgcgttaata ctttcgtcat
     7861 cgcccagcaa cagttctgcg ccaggttttg gcgctttcga ggcgcgaata tgcgcaagaa
     7921 tgcgtttgtc gtcgagcatc cgctcaacca gcacttcaat cttgccgccg ctggctttac
     7981 gcccaaacag acgcgccggg atcacgcggg tattattaaa aaccagaaga tcgccggggt
     8041 tgagcttatc gagtaaatcg gtgaaagtac cgtgcgtcag cgcgcccgtc ggcccgtcca
     8101 gcgacagtaa acgacagcta ctgcgttcag gcatgggata gtgggcaatc agggattcgg
     8161 gcaattcaaa ggagaaatcg gtaacgcgca tgacactgac tcagactaaa ataagaggcg
     8221 ggtagtctag tgccggggcg ctctccctgc aacctttacc cccctttgga tagaatataa
     8281 tcgatgaatt ttttagctca cctgcattta gcccatctcg cggaaagctc gctttccggc
     8341 aatttgctgg ctgatttcgt acgcggaaat cctgaagaaa gttttccgcc cgacgtcgtg
     8401 gctggcattc atatgcatcg gcgtatcgac gtactaaccg acaatctgcc ggaagtccgc
     8461 gaagcacggg aatggtttcg tagtgaaact cgccgcgttg cgcctattac gctggatgtc
     8521 atgtgggatc actttctttc ccgccactgg tcgcagctgt cgccggactt tccgctacag
     8581 gaatttgtct gttatgcccg cgagcaagtg atgacgattt tgccggactc accgccacgt
     8641 tttatcaatc tgaacaatta cttgtggtca gagcagtggc tggtgcgcta tcgcgatatg
     8701 gatttcatcc agaacgtgtt aaacggcatg gcaagccgtc gcccacgtct ggatgccctg
     8761 cgtgactcct ggtacgattt agacgcccat tacgatgctc tcgaaacccg cttctggcag
     8821 ttttatccgc ggatgatggc gcaggcgtca cgcaaggcgc tctgaaactt caagctccgg
     8881 cgtcactatt ttttagcatt cgcatccagc ggagcggcat aacaactgta agaaaaacat
     8941 tcgctggaat gattggcgat atctttaaag ttgctttcag caaccccctc gaacatgagt
     9001 accgttctgg acaactctcc gttgtcattt atatgtggaa aatcaaattg tgtactgaat
     9061 cgccatacat ctgtcttttc tggctcgctg ttcacagaga tacgtcgatg gaagactcct
     9121 cctgaaaacg tgcgccagaa caattgttga tcaaaatctt gtaatatctc agagttggat
     9181 gctatttgat gtagtaaacc tgcataccgt ggactgccat tacgagcaac attatgcaaa
     9241 ccctgactaa aagcatcaaa caaacaatag atgtgatcac tagtgaaaaa aggtcttaat
     9301 tttttttgtt cttcatcgct gtaatccagg agcatctcat tgatttcttc cggtgagaat
     9361 tttaagacaa aattatcctc aggatttgtt tctttcttgt ttttttcact attcttctcc
     9421 tcaatggtct tcgcattttg tttcgcaatt ttttcctcag tttttttcgc aattttttcc
     9481 gcaatttttt cccgagtttt tgattttaga ctaacaccat cgatgtcaac cgatgttgtt
     9541 ttcagatctc tctgcgcgtc tttcaaattt gtcttataga gatcaggcac tttatcggca
     9601 gataagtatc cggatgaaga aatctggtgt gtacgcatgg ggggctgttc acaaagcatc
     9661 gaggctatat cgtctttaaa ctcttttaca ttaaagttat cggcattaac cttccctgtt
     9721 ataaaccccg catcggcaat tttcttctct aattgttttg tacgtttaga ataataagcg
     9781 ttgttatcat aaaacttttc aagaagggcc cactcctcaa gttgccttat gtgatttttc
     9841 aactgttgat gatctatttt atattcatgt gtactagaat cattttggtt gcaagagtta
     9901 cagattttta agcataattt tccatcagat ttatcatcta tggtatcagt gaaaatatag
     9961 gacacttcaa tattctgccc gttcttatca gtcatctcat atgaccattg ccccgttcgt
    10021 ttcgcattct taatgagttc gatggactct gcaggacaac catgaagaat gggcagaatt
    10081 ttatcaggat gcttaatgag taaaattatt atatttacaa tgtttgaaaa gaaatctttg
    10141 ctatttttta tttcatcgat tttttccgct gaaatgttta ctaagttttc atttccggaa
    10201 atgaaaagca atttgccatg ggggcttttt ttaaattgtt cttctgtttt agaaaaaaca
    10261 tcctctttaa aaggtggcaa ctcagtacca ggaataaacg gtttcaataa attcgaattt
    10321 gaaattgtca tatctttgct ccactacagg gttatcctgg agcatgttgt acataacctc
    10381 attggcaaag aataccaaaa atcgctcata tcgaacggga caaatattta acaaactcag
    10441 ccctgacatt gagcagcttt tttgcgctgc gtagtgtcag ggctggtgac tacgacgtaa
    10501 taaataaaac agatgcttta gttgatccac accgtagccg aaatggcagg caaagccaga
    10561 atcccgtctg tcagttgccc atggccttct ttgcgttgcc attgcgcaac attaagcaac
    10621 ggtgacgcgg gtagcaccac ttcacaggct tctccacggt tgattgccac cagtacacgc
    10681 tgctgattca gcacgcggcc aaataccacc acgttatctt ccgcatacag cacctgacag
    10741 ccgccacgac gcagcgcctg acttttctta cgcagcgcaa tcattcgctg gtacagcgcg
    10801 aataacgccg tatcctgctt ttccacctgc caggggaacg gtttacggca aaacggatcg
    10861 tttttaccat ccagccccac ttcatcaccg taataaatgc acggtacacc aggccaggtg
    10921 aacaaccaga ccaccgccag cggcaggcgc gcaatatccc gaccgagcag cgttttaaat
    10981 cgcgcagtat cgtggctatc gagctgatta aacatacgca gttgctgttg atgagaaagt
    11041 ccggcgcggt aattatccat ccaggccata caggtttggg cgtcaatttg ctgcggatca
    11101 taagagatgt cggtattggc aagaaatccc cacaacggga atgtgaagcc gcgatagttc
    11161 atggcggcat cttccacatc ggcctgtaac cattgccgtg catcgccaaa atgctctcca
    11221 acgatatacg cttccggctg ggtttctttc gccgcttcgg tgatcccggc aacgtgctgc
    11281 aaattattgc gcgccccacc cgcttccccc agcatatgca ccacatccag ccgccagccg
    11341 tccatattcc acggcgcttt cagccagtgg cggacaatac tgtcttcccc gcgataaatt
    11401 tcattcacca gactttccga ctgataatcc agtttcggca agctggcata gcccagccag
    11461 tcgagcgccg tgccatcgtc actaaacgag taccagtcgc gccagggcga ttcggggttg
    11521 tgacaagcac cacccgtgcc acgattgtgc ctgtcaaacc aggcatggga atcgccactg
    11581 tggttaaaca cgccatccag caccagccgc attcccagct gctgcgtatg gtgtcgcaaa
    11641 cgcagcaacg ctccgtcgcc gccaaactgc ggatcgacgt ggcgataatc ctctgtatcg
    11701 tatttatgta cgctgggggc tttaaacacc gggttaagat acagcgccgt cacgccgagc
    11761 tttttcagat acggcagttt ttcgctgata ccgtccagat cgccgccata gaacgttgat
    11821 ccgcccgcct gcgccgtgac cggttcatcc cagtcacgca atatgatctc ttgtccggct
    11881 gcgtgatggt aataaacatg atcctgttca gcttcacgag gaagactacg ggcgaaacga
    11941 tcagggaaga tctgataaaa aatctgatcc gcagcccatt gtgggccgat atccgggaca
    12001 tcgacagcaa actgctccag tcgtgctggc ggcattcggc tgaagccctg cggtgtaaac
    12061 caacgctggc gatcgtgcca cagtaatttg aaactataac gccgccgagg ttg
//
LOCUS       DAMZFT010000141        12061 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.141, whole genome shotgun sequence.
ACCESSION   DAMZFT010000141 DAMZFT010000000
VERSION     DAMZFT010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12061)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12061)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12061
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.141"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(<1..135)
                     /locus_tag="PN977_004106"
     CDS             complement(<1..135)
                     /locus_tag="PN977_004106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001229404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3262 family protein"
                     /protein_id="HDI6044084.1"
                     /translation="MALTSAQITAFKVASGDVDLNVVHLTSVGMLVAVLFLWAAWGLS
                     D"
     gene            complement(135..476)
                     /locus_tag="PN977_004107"
     CDS             complement(135..476)
                     /locus_tag="PN977_004107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000492098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6044085.1"
                     /translation="MFSLPAFPRRAAALAGTTGMMLLAIVAVPVQAAEKDELATAMRQ
                     LDQVRAALDRARTASLQATPHDGRYFFDYPRAQADIQTMQAGIDRYLTPSRAQPRNGS
                     AVTGEYRRERP"
     gene            720..911
                     /locus_tag="PN977_004108"
     CDS             720..911
                     /locus_tag="PN977_004108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000394957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6044086.1"
                     /translation="MEFLLTALPYRALFSLTWFFVVLYIVREEPQCPVWLYDVIRGIN
                     LLIIVITIVVIPLLPVLLR"
     gene            complement(995..1756)
                     /locus_tag="PN977_004109"
     CDS             complement(995..1756)
                     /locus_tag="PN977_004109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001422158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03747 family integrating conjugative element
                     membrane protein"
                     /protein_id="HDI6044087.1"
                     /translation="MAEARRDAERSPQSRAPRQHGLLYNLVWGWPWALVGLILSSLLL
                     SLLIEYAGMTFFWSEEGAAHSRAVMNTELGWLSTEFTRSLLLSAPAETVPAWISAAYQ
                     WLFVDSGMQTWLHAQQAGTAAGRSVSEFSSELSQWLAYHLHDYLLATVYVTVVTIVRV
                     VILVLSVPLFVMVVLVAVVEGLGRRDLRRYGAAYESSFVYHHAKKSVRPAIYVPCIIY
                     LSWPGAVYPNLLIWPAAMLLGLAVTVTVASFKKYL"
     gene            complement(1737..3854)
                     /gene="traD"
                     /locus_tag="PN977_004110"
     CDS             complement(1737..3854)
                     /gene="traD"
                     /locus_tag="PN977_004110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001681762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system coupling
                     protein TraD"
                     /protein_id="HDI6044088.1"
                     /translation="MSNRYVIEALLRPAVEFNTAVVAATAACICVTAPWAVALAPTVS
                     YATAGGFALLAVVRFRQGMNIIRYRRNLRRLPRYVMSTRQIPLSRQRLFLGRGFRWTQ
                     KHTQRLQDTLRPEVAHYLQPGGLYRLARWLEMKTEHRLPALGKLLRHESSLNPVRPLP
                     PVGGNPALHGIEPDEQDVTLALGERVGHTIVYGTTRVGKTRLAELLVTQDIRRGEVTI
                     VFDPKGDADLLLRVWAEAHRAGRGDELYIFHLGWPEISARYNAVGRFGRVSEVASRVA
                     GQLSGEGNSAAFREFAWRFVNIISRALVALGERPDYLLIVRYVNNIADLYIRYAGKVI
                     RERMPELEQAIANNQNVISEEDVPRTMQNQPDAVRIWSIEMALSSEEGKKIYDPILDG
                     LRSAVRYDRTYFDKIVASLLPLLEKLTTGRIAELLAPDYLDMNDARPIFDWEQVIRKK
                     AVVYIGLDALSDSVVASAVGNSMFADLVSVAGHIYKHGMHAGLPGKGEGKNVVNLHCD
                     EFNELMGDEFIPLINKGGGAGMQVTAYTQTSSDIEARIGNAAKTAQVQGNFNNMIMLR
                     VRENRTAELLTTQLPQVEIYTKTLVSGHQDTADVSAGQDFTSSTQDRVGTVKVPLLEP
                     SDIVTLPKGQAFALLEGGQLWKIRMPLPAGDTDDVLMPENIARVAEAMRQNYHTGEGW
                     WNEPPSAQAAGTDEGGGYGGSAS"
     gene            complement(3854..4603)
                     /locus_tag="PN977_004111"
     CDS             complement(3854..4603)
                     /locus_tag="PN977_004111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006858941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6044089.1"
                     /translation="MPQWANDPLLAWDATAFAAMSDFITEHYWTGQGSINVFRIVGTD
                     HPQYAGMTWLELLERGNRMDINIPLLEKNPGYYTQAEQQHAGMSFVSTDGVDWYVSAD
                     GNHRSCLARFLFHLQGEERTQLHNVAQSIYHTDRAFWSACREIHNLTDALSRHGVYIR
                     LQTRRQCVAREDLACWKVDRFSTEAQLTVDDVRAGGHDMPAVYKTLLLNAADAWREVM
                     VLQRRLEALSASPGNDLPRTWWRRLLRKGDM"
     gene            complement(4636..5148)
                     /locus_tag="PN977_004112"
     CDS             complement(4636..5148)
                     /locus_tag="PN977_004112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000989255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6044090.1"
                     /translation="MMTVKRMFMFLFRITIVWPSVLTGCVMMLYFAKVAGGGVAGLEQ
                     RYGVESARWRDAPEGMVMTVQCPSRPFMASVSTGEWRALAASDDECSQVSRSFREAAE
                     GDAQSLRRVAVTLLLASVVLEAFSACILRRVTASVRVSPHCGYGCGRGAVAHWPESRV
                     HAAPEKGERV"
     gene            complement(5145..5735)
                     /locus_tag="PN977_004113"
     CDS             complement(5145..5735)
                     /locus_tag="PN977_004113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006858939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HDI6044091.1"
                     /translation="MQRQLTDLLQEGAWHGILLCVLVVLFLFFPRLWGRCRHRERASE
                     RRHRRYRAAAERVLTKLPEGGAGVKYLRKINPYVFEELLLLAFERRGMAVIRNTRYSG
                     DGGLDGQVLIDGERWLIQAKRYGRTIRPSHIDDFGDLLRREGCRGFFIHTGRTGPASR
                     ARLALWPDIELVSGQRLLKLLNGALLPDELTRGARR"
     gene            complement(5735..6262)
                     /locus_tag="PN977_004114"
     CDS             complement(5735..6262)
                     /locus_tag="PN977_004114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006858937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrating conjugative element protein"
                     /protein_id="HDI6044092.1"
                     /translation="MKRFKRYVMTTATGLLLCAAQAGAELRVIADLGGSDAGRFYEAI
                     NRQADSGTSSPVTALLPQGESAMLPVSTPEMSPGEVTPRPLQLPGLGALFLVGDDALS
                     RQWLQANAAALLERKAAGMVVSVTNESGLQALRALAPGVMMVPASGSALARRLQLDHW
                     PVLITDSGISSVVMP"
     gene            complement(6259..6915)
                     /locus_tag="PN977_004115"
     CDS             complement(6259..6915)
                     /locus_tag="PN977_004115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000090711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HDI6044093.1"
                     /translation="MARGSLLMSLLLMAVSIPAGAALHDIPEGYVRVAEQHGVPAEVL
                     YAVSLTETAMVPSAIGAVMKKRNEDYRVPAVARPWPWTINVAGRGYRYASRLEAWQAL
                     QVFLKRYPARRIDVGIAQVNLGWNGHRFASTWAAFDPYTSLNTAAVILRECRRRHPDS
                     WLQAAGCYHHPAGGAPAVRYIALVRRHIDRLHRDAVSTSGVTAMNPRYIRTEPRELTP
                     "
     gene            complement(6894..7613)
                     /locus_tag="PN977_004116"
     CDS             complement(6894..7613)
                     /locus_tag="PN977_004116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006785960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03759 family integrating conjugative element
                     protein"
                     /protein_id="HDI6044094.1"
                     /translation="MKLKSLSLALMLLSLQAMAAVQESTQQGTRTSSSSQVSTTTQEL
                     QQQAGQWGLSEEDYRRYRTLMEGPRGIQSPGLDPLTVLGIEARTDAERRVLAEKWVKA
                     EYERTEKELAFQREVNAAWKRLYPGVMPVSLGNSAGIARDTGGRLALFVRSKDCSTCD
                     ARLAAVLSSGRQVDIYLVDSQGNDEKLRQWAREHSIPADRVRSRHITLNHDGGRWLRF
                     GEGRMPVVLQQGADGWRVAAF"
     gene            complement(7618..8397)
                     /locus_tag="PN977_004117"
     CDS             complement(7618..8397)
                     /locus_tag="PN977_004117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001696689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6044095.1"
                     /translation="MSEKQQEISAAAGGSGGTTPSGRAKRRPSPGLFAGLVTVVMMAI
                     AVAGLARYTGEQDRRMTALAVQMKAYSDALALSGTELVPLRERLAETEKQLKSVAERI
                     SQLEKRPALTEEEHARTGEMAASLASLQTSLAQQGETLRTYEQRLEKVEKTSVTPVSP
                     SSAKGAGGTMKGTAAVAPQKKRMSAGVPVVSRTAPFVLTGTEQRGGRTYAAVAPRGFS
                     SLADVTLLGEGESTTGWVLERAGRGEATFRVNGRQVRVMVE"
     gene            complement(8394..8972)
                     /locus_tag="PN977_004118"
     CDS             complement(8394..8972)
                     /locus_tag="PN977_004118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006858932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilL N-terminal domain-containing protein"
                     /protein_id="HDI6044096.1"
                     /translation="MKISLLFSALSVCVLLAGCQSPQKLQQRAAAVPVPSVTVSNNVR
                     PANPDVYAGAATPEVVRYDRYLLVNTAPDTVQRDPLSQVIDIRIPASLKPTVADAMRY
                     ALKQSGYTLCATGPANGVLYRQPLPAVQYQTGPVRLRSALQMMAGPAWQLEVDDVQRV
                     VCHSLREGYQLPARQLAPVPAVVKVSGAEQKK"
     gene            complement(9163..9411)
                     /locus_tag="PN977_004119"
     CDS             complement(9163..9411)
                     /locus_tag="PN977_004119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006858920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDI6044097.1"
                     /translation="MDFAELSEAIFTHYPSHKGVIMTIAEQLEEKGLEKGRAEERQKA
                     LAETYASVRRMSDMGMSTEVIKQALQLSDEQIQEALNN"
     gene            complement(9545..10525)
                     /locus_tag="PN977_004120"
     CDS             complement(9545..10525)
                     /locus_tag="PN977_004120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001354420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HDI6044098.1"
                     /translation="MSEKVNKITMIHDGAFKKTLEDPAAVRDFLEQVLTHDQISRCNL
                     DTIEVEKNSFVTESLRQHACDVLISMKTNDGRDGYIYTLIEHQSSPDKFIPLRMMRYI
                     LAVMEQHVEKHKCAPVVIPVLFYHGAMRPYPYPLNWVDCLDDPEYGREIYGESKSFSL
                     VDVSTLDDDEIEQYRRMAALMYTMKSGTQGDLIELIGKSITLTDKYGSSIHLNTVLTY
                     LMELYQMDVAELFEAISTHYPSHKGVIMTIAEQLEEKGRVEGLEKGLEKGLEKGLEKG
                     RAEGRAEERQKALAETYASVRRMSDMGMSTEVIKQALHLSDEQIQEALNN"
     gene            complement(10572..11129)
                     /gene="ssb"
                     /locus_tag="PN977_004121"
     CDS             complement(10572..11129)
                     /gene="ssb"
                     /locus_tag="PN977_004121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004115562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HDI6044099.1"
                     /translation="MASRGVNKVILIGHLGQDPDIRYFPNGGGAVANLSLATSDTWRD
                     KQSGEMKEVTEWHRVVLYGKLAEVAGEYLHKGSQVYIEGQLKTRKWFDQQNGVDRYST
                     EVVVGVNGVMQMLGSSRQQGASGNGASALAQGGQGHPPKTTGQTGRATRTTSTGNGAP
                     PMDFDDDIPFMGLGYGMERRIIHAM"
     gene            complement(11142..11345)
                     /locus_tag="PN977_004122"
     CDS             complement(11142..11345)
                     /locus_tag="PN977_004122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000204786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6044100.1"
                     /translation="MTSTNGILAAAAGYTLPLEILKSGRGYYIGTQCSEGPVSRESEE
                     YFKKYEQAERALKNGTWTQRHAW"
     gene            complement(11445..11909)
                     /locus_tag="PN977_004123"
     CDS             complement(11445..11909)
                     /locus_tag="PN977_004123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016151303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3577 domain-containing protein"
                     /protein_id="HDI6044101.1"
                     /translation="MSATNSTSAKTEYFNLTMKGLGYLSNVRQVNHQNGSFLSCVINA
                     LSGPTDNPSYTRFDVTVAGKEATTLISRCQKAVDEDRKVLLGFTLSNLATDIFTLNKG
                     EHAGEQRVSLKARLIKVDWIKIGQEQVYKAEKAESQPPSHGTTKREYAEDSF"
BASE COUNT         2622 a         3827 c         2998 g         2614 t
ORIGIN      
        1 atccgacagt ccccaggcgg cccagagaaa gagaacggca accagcattc ccaccgatgt
       61 cagatgcacc acattcagat ccacatcacc ggatgccacc ttaaaggccg tgatctgtgc
      121 cgacgtcagc gccatcaggg acgctcccgc cggtattcac cggtcacggc actgccgttt
      181 cggggctggg cacgggacgg cgtgagataa cggtcaatac ccgcctgcat ggtctgaatg
      241 tcagcctgtg cgcgcgggta gtcgaagaaa taacgcccgt catgaggcgt ggcctgaagg
      301 gatgccgttc tggcacggtc aagcgccgcc cgtacctggt caagctggcg catggccgtt
      361 gccagctcat ccttttcggc ggcctgaacc ggcacagcca caatggcaag aagcatcatc
      421 ccggtagtac ctgccagggc agcggcacgc cggggaaatg cgggaagaga aaacatacgc
      481 acctccgtta agaatctgtg ggtgcgtaca gcatgaagaa ggcaggtgtg ccggtcagcg
      541 ataaactcta tttgtaaaac tgaaaatttt taagggaaaa cggacatggc tgaatgccgg
      601 aacatatccc cgcgccgggg cgcaatttta ttccatcttt aatgattttt tctgtttact
      661 gaaacaggtc atcactacag tttcccgcta atgacccccc gtaaagagga aacatcagca
      721 tggaatttct cctcaccgcc cttccgtacc gggccctgtt cagtcttacc tggttttttg
      781 ttgtgttata tatcgtcagg gaagagccac agtgccctgt ctggttatat gacgtcatca
      841 ggggcatcaa cctgctcatc atcgtcatca ccattgttgt cattcccttg ctgccggtgc
      901 tcttacgctg acgcgctgtg acagtcagta cgtggcttgt ctgccgacac cccgccacaa
      961 aacacgtttt gcaccggggt gtccggcgca tgaatcagag atattttttg aacgatgcca
     1021 cagtcacggt aaccgccagt cccagcagca tggccgccgg ccatatcagc aggttcggat
     1081 ataccgcgcc gggccaggaa aggtaaataa tgcaggggac ataaatcgcc ggtctgactg
     1141 atttttttgc atgatgatag acaaagctgg actcataagc cgcgccgtaa cgccgcaaat
     1201 cacggcgccc cagcccctcc acaacggcca ccagaaccac catcacaaac aacggaacgg
     1261 acaggacgag aataaccacc ctgactatcg tcaccaccgt cacatacacc gtcgccagca
     1321 ggtagtcatg aagatgataa gccagccact gactcagctc actgctgaat tcagacacgc
     1381 ttctgcctgc cgccgtcccc gcctgctgtg catgcagcca tgtctgcatg ccactgtcca
     1441 caaacagcca ctgatacgcg gcggaaatcc acgccggcac cgtttctgcc ggcgcggaaa
     1501 gcagcagact gcgggtgaac tccgttgaca gccagcccag ctccgtgttc atgacagcgc
     1561 ggctgtgcgc cgccccctcc tcactccaga aaaacgtcat accggcatac tcaatcaaca
     1621 gactcagcag cagggaggat aaaatcagcc ccaccagtgc ccacggccag ccccacacca
     1681 gattgtacag cagaccatgc tgacggggcg cccgggactg cgggctgcgt tccgcatcac
     1741 gacgcgcttc cgccatatcc gcctccttca tctgttccgg cggcctgcgc tgacggcggc
     1801 tcattccacc atccttcacc ggtgtgatag ttctgtctca tcgcctctgc cacccgggca
     1861 atgttctccg gcatcagcac gtcatccgta tcccctgccg gcagcggcat ccggattttc
     1921 cacaactgcc ccccttccag cagggcaaac gcctgtccct tgggcagggt gacaatatct
     1981 gacggctcca gcagaggtac cttcaccgtg ccgacacgat cctgggtgct ggacgtgaaa
     2041 tcctgaccgg cagacacgtc cgccgtatcc tggtgaccgg agaccagcgt cttggtgtag
     2101 atctccacct gaggcagttg tgtggtcagc agctctgccg tcctgttctc cctgacacgc
     2161 agcatgatca tgttgttgaa gtttccctga acctgcgccg ttttggctgc attaccgata
     2221 cgcgcttcaa tatctgacga tgtctgggtg tacgcggtga cctgcatacc ggcaccgccc
     2281 cccttgttaa tgagcggaat aaactcatcc cccatcagtt cgttaaactc atcgcagtgc
     2341 aggttcacca catttttgcc ttccccctta ccgggcagac cggcatgcat gccgtgcttg
     2401 tagatatgcc ccgccacgct caccaggtcc gcaaacatcg aattccccac agcactggct
     2461 accacactgt cgctgagtgc atccaggccg atatagacca ccgccttttt acggatcacc
     2521 tgttcccagt caaaaatcgg tcgcgcatcg ttcatgtcca gatagtccgg tgccagcagc
     2581 tctgcgatcc tgcccgtggt gagtttttcc agcagcggca gcagggacgc cacgattttg
     2641 tcaaaatagg tacggtcata gcggaccgca ctgcgaagcc cgtccaggat cggatcgtaa
     2701 atttttttac cctcctcact gctcagggcc atctcgatgg accagatacg taccgcatcc
     2761 ggctggttct gcatggtgcg gggaacatcc tcctcgctga tcacattctg gttattcgca
     2821 atcgcctgct ccagttccgg catgcgctcg cggatcacct ttccggcata gcggatatag
     2881 agatcggcta tgttgttcac ataccgcaca atcagcaggt agtccgggcg ctcaccgagt
     2941 gccaccaggg cgcgggaaat gatgttgacg aaccgccagg cgaactcgcg gaaagccgcc
     3001 gagttccctt caccggacaa ctgcccggcc acacgggaag ccacttccga aacacgcccg
     3061 aagcgtccca ccgcattata tcgggccgaa atttccggcc atcccagatg gaaaatatac
     3121 agttcatcac cccgcccggc acggtgtgct tccgcccaga cacgcagcag cagatcagca
     3181 tcacctttgg gatcaaacac aatggtcacc tctcccctgc ggatatcctg agtgaccagc
     3241 agttctgcca ggcgggtttt accgacacgg gtggtgccgt agacaatggt atgccccaca
     3301 cgctccccca gagccagcgt cacatcctgt tcatccggct caataccatg cagggcggga
     3361 tttcccccca ccggcggcag aggacgcaca ggattcagcg acgactcatg ccggagcagt
     3421 ttccccagcg cgggcagacg gtgttccgtt ttcatttcca gccagcgcgc cagccggtaa
     3481 agtccgcccg gctgcagata gtgtgccacc tccggtcgca gcgtatcctg gagacgctgg
     3541 gtatgcttct gggtccagcg aaatccccgc cccagaaaca gccgctgacg gctgagcggg
     3601 atctgccgtg tactcatcac atagcggggc agccggcgca gattacggcg ataccggatg
     3661 atgttcatcc cctgccggaa acgcaccacg gccagcaggg caaatccacc ggcagtggcg
     3721 taactgacgg tgggtgccag cgccaccgcc cagggggctg tcacacagat acatgctgct
     3781 gttgctgcca ccactgccgt attgaactcc acagccggtc tcagcaatgc ctcaatgaca
     3841 tagcggttgc tcattacatg tcccccttcc gtaaaagacg tcgccaccat gtgcgtggca
     3901 aatcgttccc cggtgatgca gacagcgcct ccagtctgcg ctgtaatacc atcacctcac
     3961 gccatgcgtc tgctgcattc agcagcaacg tcttatacac cgcaggcata tcatgtccgc
     4021 cggcacgcac gtcatccact gtcagctgcg cctctgtgct gaaacggtcc actttccagc
     4081 aggccaggtc ttcgcgggca acacactgcc gtcgggtctg cagacggata tacacaccat
     4141 gccgggacag cgcgtcagtc aggttgtgga tttcccggca ggcagaccag aacgcccggt
     4201 ctgtgtgata aatactctgt gccacgttgt gaagctgtgt gcgctcctcc ccctgcaggt
     4261 gaaacagaaa gcgtgccagg caactgcggt gattgccgtc tgcactgaca taccagtcaa
     4321 cgccatccgt gctgacaaaa ctcatcccgg catgctgttg ctctgcctgc gtgtagtatc
     4381 ccggattttt ttccagtaac gggatattga tgtccatacg gttaccgcgc tccagcagct
     4441 ccagccaggt cattccggca tactgtggat ggtcagttcc cacaatgcgg aacacattga
     4501 tgctgccctg tcccgtccag tagtgctctg taataaaatc cgacattgcc gcaaacgcgg
     4561 tggcatccca tgccagtaac gggtcattcg cccattgcgg catatccctc tgcacctgtt
     4621 cagtaaatga aacgttcaca cccgctctcc tttctccggt gcagcgtgaa cgcgactctc
     4681 tggccagtgc gccaccgcac cacgtccgca gccatatcca cagtgtggtg atacccggac
     4741 ggatgccgtt acccggcgca gaatgcaggc tgaaaacgct tccagaacca cggatgccag
     4801 cagcagtgtc actgccactc tgcgcagcga ctgcgcatcg ccctctgcag cttctctgaa
     4861 agagcgcgaa acctggctgc attcatcatc agatgcagcc agtgcacgcc actccccggt
     4921 gctcacagac gccataaagg gacgcgacgg gcactgcact gtcatcacca tcccttccgg
     4981 cgcatcacgc cagcgggcgg attccacccc gtaacgctgc tccagtcccg ccacaccacc
     5041 gccggcgacc ttcgcgaaat acagcatcat cacgcatcct gtcagcacgg atggccagac
     5101 aatcgttatt ctgaataaaa acataaacat ccgttttacc gtcatcatct ccgcgctcct
     5161 ctggtaagtt catccggcag caatgccccg ttcagcagtt tcagcagacg ctgcccgctg
     5221 accagctcaa tatccggcca cagcgccagc cgtgcccggc tggcaggtcc cgtacgaccg
     5281 gtgtgaataa aaaatccacg gcagccttca cggcgcagca ggtcaccaaa atcatcaata
     5341 tgcgacggac ggatggtccg tccgtaacgt ttggcctgaa tcagccaccg ctcgccgtca
     5401 atcagcacct gaccatccag gccaccatct ccgctgtaac gtgtgttacg gatgaccgcc
     5461 atccccctgc gttcaaacgc cagaagcagc agttcttcaa acacatacgg gttaattttt
     5521 cgcaggtatt tcacacccgc cccaccttcc ggcagctttg tcagcacacg ttcagccgca
     5581 gcgcggtaac ggcggtgacg acgctcactg gcgcgttcac gatgcctgca tcgcccccac
     5641 aggcgcggaa aaaacagaaa cagcacgacc agaacgcaca gcagaatgcc gtgccacgcg
     5701 ccttcctgta acaggtccgt cagttgtctc tgcatcacgg catcaccaca ctgctgatac
     5761 cactgtccgt tatcagcacc ggccagtggt caagctgcag acggcgggcc agtgctgaac
     5821 cggaagccgg taccatcatc acaccgggag ccagcgcccg cagcgcctgc agaccgctct
     5881 cattcgtcac gctcaccacc ataccggcag ccttcctttc aagcagcgcg gccgcattcg
     5941 cctgcagcca ctgacgggag agtgcatcat cccccacgag gaacagtgcc cccagccccg
     6001 gtaactgcag tggcctcggc gtcacctctc cgggagacat ttccggcgtg gaaacaggca
     6061 gcatcgcact ctccccctgc ggcagcagcg ccgtgacagg cgacgacgtt ccgctgtccg
     6121 cctggcggtt tattgcttca taaaacctgc cggcatcact gccaccaagg tcagcaatca
     6181 ccctgagctc cgcgccggcc tgagccgcgc acagcaacag acccgttgcg gtcgtcatga
     6241 catatcgttt aaatcgtttc atggggttaa ctcccggggt tctgtccgga tataacgggg
     6301 attcatggca gtcacacctg acgtactgac cgcatcacgg tggaggcggt caatatgccg
     6361 cctcaccagc gcgatgtagc gtactgcggg tgcgccgcct gccggatggt gataacagcc
     6421 ggcagcctgc agccagctgt caggatggcg acggcgacat tctctcagga tgaccgccgc
     6481 cgtgttcaga ctggtgtacg ggtcaaatgc cgcccatgtg gacgcaaaac ggtgcccgtt
     6541 ccagcccaga ttgacctggg ctatccccac atcaatccgt cgggccggat atcgtttcag
     6601 aaaaacctgg agggcctgcc aggcctccag tcgactggca tagcggtatc cccggcccgc
     6661 cacattgatg gtccagggcc agggacgtgc cactgctggt accctgtaat cctcattccg
     6721 ttttttcatt acagcaccaa tggcggaggg gaccatggcc gtctcggtca gcgagacggc
     6781 atagagtact tctgccggaa caccatgctg ttccgccaca cgcacgtatc cctccgggat
     6841 atcatgcagc gccgcccccg ccggaatgct gactgccatc agcagcagcg acatcagaag
     6901 gctgccacgc gccatccgtc agccccctgc tgaagtacca ccggcattct cccctcccca
     6961 aaccgcagcc agcgaccgcc gtcatggttc agcgtgatgt ggcgactgcg cacacggtct
     7021 gccgggatac tgtgctcccg ggcccactgc ctcagctttt catcatttcc ctgactgtcg
     7081 accaggtaaa tatccacctg acgaccggat gaaagcacgg cggccagccg cgcatcacag
     7141 gtactgcagt ctttcgacct gacaaacagg gcaagacgtc ccccggtgtc acgggcgatg
     7201 ccggcagaat tccccaggct gaccggcatc acccccggat acaaccgttt ccaggcggca
     7261 ttcacttcac gctggaaagc cagttccttc tcagtgcgtt catactcagc tttaacccat
     7321 ttttcggcca gcaccctgcg ctctgcatcc gtccgcgctt caatccccag taccgtcagc
     7381 gggtccagcc ccggagactg aataccgcgg ggaccttcca tcagggtacg gtaacgccgg
     7441 taatcttcct cactgagtcc ccactgcccg gcctgctgct gcagttcctg tgtggtggta
     7501 ctgacctgag aggatgagga ggtgcgcgtt ccctgctgcg tactctcctg tacggcggcc
     7561 atcgcctgca gggacagcag catgagcgcc agtgacagac ttttcagctt cattttctca
     7621 ctccaccatg acccggacct gacgaccatt cacccggaac gtggcctcgc cacgtcctgc
     7681 acgctcaagc acccaccctg tcgtgctctc cccttcaccc agtaacgtca catcagcaag
     7741 actgctgaat ccacgggggg ccactgccgc atacgtacgg cctccccgct gctctgtccc
     7801 ggtcagaaca aacggcgcgg tacgactgac caccggcacg ccggcagaca ttcgtttctt
     7861 ctgtggcgca accgccgctg tgcccttcat cgtcccgccc gcgcctttcg cagatgacgg
     7921 tgataccggg gtgactgaag tcttctccac cttctccagt cgctgctcat acgtgcgaag
     7981 cgtttctccc tgttgcgcca gtgaggtctg cagtgatgcc aggctggcgg ccatctctcc
     8041 cgtacgcgca tgctcttcct ccgtcagtgc aggacgcttt tccagctgag aaatacgttc
     8101 cgccacactc ttcagctgtt tttcggtctc agccagcctc tccctcagtg gtaccagttc
     8161 cgttccggac aaggcgagcg catcagaata cgccttcatc tgcaccgcca gcgcagtcat
     8221 tcgcctgtcc tgctctcccg tataacgagc cagaccggca accgcgatcg ccatcatcac
     8281 caccgtcacc agccctgcga acagaccggg agagggacgc cgtttcgccc ttcctgacgg
     8341 tgtggtgcca cctgaaccac ctgcagcggc tgatatctcc tgttgctttt cactcatttt
     8401 ttctgctccg caccggatac cttcaccacc gccggtaccg gcgccagctg gcgtgccggt
     8461 aactgataac cctcacgcag actgtgacag accacgcgct gcacatcatc cacctcaagc
     8521 tgccatgccg gccccgccat catctgcagc gcgctccgca gtctgaccgg cccggtctgg
     8581 tactgcacgg ccggtagcgg ctgacggtag agcacaccgt tggcaggccc cgtggcgcag
     8641 agtgtgtagc cggactgttt cagggcataa cgcatcgcat ccgccaccgt cggcttcagt
     8701 gatgccggaa tgcggatgtc aatcacctgg gaaagcgggt cacgctgtac cgtatccggt
     8761 gccgtgttaa ccagcagata gcggtcataa cgcaccacct ccggtgtcgc cgcaccggcg
     8821 tacacatccg gatttgccgg acggacattg ttgctgactg tcaccgacgg taccgggacg
     8881 gcagccgccc tctgctgcag tttttgcgga ctctggcacc ccgccagcaa cacacagaca
     8941 gacagcgcgg aaaaaagaag ggatattttc atcagggaat tacctctgca gtgaagtgat
     9001 aaagtcagag gtcaatttgc agaagaagtg aggggaggct caatgaacaa ctgatttccg
     9061 gttgacaaaa aaaacgcctg cacaatggca ggcgctgtgt tcacagggca tcacgcattc
     9121 tgacgcatca gtcgcgctct gccacatcat tacgatgtga ggttaattat taagcgcttc
     9181 ctgaatctgt tcatcagaaa gctgaagagc ttgcttaata acttctgttg acatccccat
     9241 atcactcatg cgacggacag atgcataggt ttctgcaagc gctttttgac gctcttctgc
     9301 acggcctttc tcaaggcctt tctcttccag ttgttccgct atcgtcataa tcaccccctt
     9361 gtggctggga tagtgtgtga atattgcttc agaaagttca gcaaaatcca tctggtaaag
     9421 ttccatcaca atttaccaga ctgattgata aaaaaacgcc tgcacaatgg caggcgctgt
     9481 gttcacaggg catcacacgt tctgacgcat cagtcgcgct ctgccacatc attacgatgc
     9541 gaggttaatt attaagcgct tcctgaatct gttcatcaga aagatgaagt gcctgcttaa
     9601 taacttccgt tgacatcccc atatcgctca tgcgacggac agatgcatag gtttctgcaa
     9661 gcgctttttg acgctcttca gcacggcctt cagcacggcc tttctcaagg cctttctcaa
     9721 ggcctttctc aaggcctttc tccagacctt caacccgacc tttctcttcc agttgttccg
     9781 ctatcgtcat aatcaccccc ttatggctgg gatagtgtgt ggatattgct tcaaaaagtt
     9841 cagcaacatc catctggtaa agttccatca gataagtaag caccgtattc aggtgaatgc
     9901 tactgccata cttatccgtc agtgtgatcg atttaccaat cagctcaatc aaatcacctt
     9961 gagttccgga cttcatggtg tacatcagcg ccgccatccg gcgatactgc tcaatttcat
    10021 catcatcaag cgtactgaca tccaccagac tgaatgactt tgactcgcca taaatctccc
    10081 gtccgtactc cgggtcatcc agacaatcca cccagttcag cggatacgga tatggccgca
    10141 ttgcaccatg ataaaacaac acggggatca ccacaggtgc acacttatgc ttttcaacat
    10201 gttgctccat tacagccagg atatatcgca tcatacgcag cgggatgaat ttatccggcg
    10261 aactctggtg ctcgattaag gtataaatgt atccgtccct gccgtcgttt gtcttcatgc
    10321 tgatcaaaac atcacaggca tgctgacgca aactttccgt cacgaacgaa ttcttttcaa
    10381 cttctattgt atcaagatta cagcgggata tctgatcgtg tgtcaagacc tgttcaagaa
    10441 agtcccttac tgcggcggga tcttcaagcg tcttcttgaa cgccccgtca tgaatcatag
    10501 tgattttgtt aactttttca ctcacggaac ctccggataa aaaagtgggg cgtaacgccc
    10561 cgtggttaat attacatggc atgaataatt cgtcgttcca tgccataccc gagccccata
    10621 aaggggatat catcgtcaaa gtccattggt ggagcaccat tacccgttga cgtcgtccgg
    10681 gtggcacgac cagtctgtcc ggtcgtcttc ggcggatgtc cctggccccc ttgtgccagt
    10741 gctgaagcac cattaccgga agcgccctgc tgacgcgatg aacccagcat ctgcatgaca
    10801 ccattcacgc ccacaacaac ctcagttgaa taacggtcca caccattctg ctggtcaaac
    10861 catttacggg ttttaagctg gccttctata tacacctgcg accccttatg caggtactcg
    10921 ccggcaactt cagccagttt tccatagaga acaacgcggt gccattcagt aacttccttc
    10981 atttcaccgc tctgtttatc ccgccaggtg tcgctggtcg ccagtgacag attcgccaca
    11041 gcgccaccgc cattggggaa ataccggata tccggatcct gccccagatg tcctatcagg
    11101 ataactttgt ttacgccgcg tgaagccata gcacctcctc attaccaggc atgacgttgt
    11161 gtccaggtgc catttttcag cgcccgctca gcctgctcat attttttgaa atactcttct
    11221 gattcacggg ataccggtcc ttcagaacac tgagtgccaa tataatatcc gcgcccactt
    11281 ttgagtattt ccagaggtaa tgtataacct gccgcagctg cgagaatacc gtttgtagaa
    11341 gtcataattt gtttccttaa agttcagtaa aaacaataag aaaacagacc tccctgatgg
    11401 tggtctgttt tctttatcag ggaggcagtg agaaaaatac gcgatcagaa ggagtcttct
    11461 gcgtattcac gttttgtggt gccatggctc ggtggctggg actccgcttt ttcagcctta
    11521 tagacctgct cctggcctat ttttatccag tccaccttga tcaggcgggc tttcaggctg
    11581 acgcgttgct caccggcatg ctcccccttg ttcagggtga aaatgtcggt ggcaaggttg
    11641 cttaacgtaa aaccaagcag taccttgcgg tcctcatcca ctgctttctg gcagcgggag
    11701 atcagggtgg tcgcctcttt accggcaacc gtgacatcaa aacgcgtata ggacggattg
    11761 tccgttggtc cactcagtgc gttaatcacg cagctgagga acgaaccgtt ctgatggtta
    11821 acctgacgta cattgctgag atacccaaga cctttcatgg tcaggttgaa gtattcagtt
    11881 tttgcagaag tagagttagt tgcagacatg atgatttctc catggagtaa aaaaagatat
    11941 gacggagaac atcaccagcc ctgacgggag tgatagttcc cgtcggggat gccggaatca
    12001 gctgaatttc cggctgtctg tgcagttaaa cgtacctcaa tcaccagtgg tgatatccgt
    12061 t
//
LOCUS       DAMZFT010000142        11984 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.142, whole genome shotgun sequence.
ACCESSION   DAMZFT010000142 DAMZFT010000000
VERSION     DAMZFT010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11984)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11984)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11984
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.142"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(<1..502)
                     /locus_tag="PN977_004124"
     CDS             complement(<1..502)
                     /locus_tag="PN977_004124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001407399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion protein E"
                     /protein_id="HDI6044102.1"
                     /translation="MLDPADLPPDDILDYVAIDTDKTGHLRVRVVEGKKHLRAVQEYL
                     TRLRARHQGRVGDFEFTTLDVIARLRQDTTTAGDESVINPVQQKMLGYIRHSASLGAS
                     DLHMTPGRDNTDFTYVEARVHGELEVLDILRKEEGLELLGATYSGMTDVIKGTQFDPG
                     VPQDARL"
     gene            complement(599..1195)
                     /locus_tag="PN977_004125"
     CDS             complement(599..1195)
                     /locus_tag="PN977_004125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000892312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6044103.1"
                     /translation="MLENKTTDDFIFVKQHIYRIFQWMHETPLINNGNTPVTDRLIAD
                     IRWMLEHHYYPSPGQVDRIISVLRETKFQLLREKTDRQELVEEIQQKLNTLKDIRHFL
                     KLNTNYKQDGDIISLTPSGEWKNTSIILSVVAVMLMIFASVCNLSGITPYFIYTAGII
                     FYALATPVLTLRSTQPEKNIPLLFAFGGVIIFTLLSLC"
     gene            complement(1291..1845)
                     /locus_tag="PN977_004126"
     CDS             complement(1291..1845)
                     /locus_tag="PN977_004126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001026779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6044104.1"
                     /translation="MNISELISWLSLIIRDLETAAAEYGVNHTDIVHEATQLQVQLCR
                     GKQVTPAQLRALSARLWGARMRLAAQYGQDAPLMNDLAFLSNCLKYDADRLNDRWLYR
                     EWISAAESFVLPLVFIIPLLIALCYMMKSGNSGGAELCAALAGAWCTGLTFLYLWAKD
                     PVGLFWSLYSFIPLYLLWCDISPA"
     gene            complement(1894..2346)
                     /gene="pilP"
                     /locus_tag="PN977_004127"
     CDS             complement(1894..2346)
                     /gene="pilP"
                     /locus_tag="PN977_004127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000543062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus biogenesis protein PilP"
                     /protein_id="HDI6044105.1"
                     /translation="MHAKTKALLVSALLLPATAMATTGNAEEQSPRQLQIERIQDETA
                     LYEAMLAREKIRHELRMLEQGIPAEPGRPQESTSAIPTAPSTATSPRLTEIFGVQPQL
                     RARIELSGGHITDVKTGQNIPGTPYLVEKITPDSVVLEQSGIRKTLRP"
     gene            complement(2336..3631)
                     /gene="pilO2"
                     /locus_tag="PN977_004128"
     CDS             complement(2336..3631)
                     /gene="pilO2"
                     /locus_tag="PN977_004128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001681793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 4b pilus protein PilO2"
                     /protein_id="HDI6044106.1"
                     /translation="MTDISPTERQVFPLPAGRNVFLAGLEWKTLPSQYRHARDFARAQ
                     KADLFLACQYLSNEDADTHTMVATVSRRILPGKPRQCFSLALLILPLLEHGGYAITEL
                     TLPGETPRYSFVSAVDGVLVSDLVGSGEEVREARDTFLSINTEPEQGWTRYEPVAFSA
                     GDQNQALPLSTLTGSGKHPAAARLNPVSRGAQFITVSLIIALLGAAWYGWQYYERWKT
                     EQAAQAAAARQVTEEQISPPWPGLPETDAFIQGCADIWTSLPLSASGWRYSLAECSTD
                     GGSGNLRASYTNTAGATVTDFIRRISDMTGTRPFIVMPEGKTGGIGLPVSFRLPEHPV
                     DVDALPESSVLQEQLTTLAQSMPLTLDWQEVSNSVTDENGNIIQPPWKEYDLQIQTLL
                     PAYQLVERLKAPSVRFISVTRQLENGRFHYQFTGKYYAR"
     gene            complement(3652..5271)
                     /locus_tag="PN977_004129"
     CDS             complement(3652..5271)
                     /locus_tag="PN977_004129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilN family type IVB pilus formation outer
                     membrane protein"
                     /protein_id="HDI6044107.1"
                     /translation="MNKKILAIMIAAALLQGCASYRQLESTRKEAVAEQQRAREMMSD
                     LRTRTPVVKESSRQWINPRPVMDKNTGKTVPGCPIVIHTRQSITLQQVVQRITESCHI
                     PVRITPDVLTYLNTSTTGSTQQIQGALPPPDASGMVPLAAIGASDTPSSRLPGNTAAL
                     SELNASGLTTLLNAVSSRLGISWRYDGSHIIFYYLETQTFPITYMDSQVTYNATVVSG
                     TMSSNGSSGGSASDAISGDASNTQTTTVDMKSALYQDVKNAVTSMLTPGIGRMFMSTG
                     FITVTDNPQVLETVRAFIEKRNEEMKRQVVLNVEILSIRKTRNEQAGIDWNAVFSDRT
                     LGLSLGSTFTSAASDAITGGVSIVDGKLTGSKAFLKALSSQGVVSVVTQHSAVTKNLT
                     PVPMQIANQQSYIESVTTDTTANVGSSTSLNAATITTGFNMTLLPFILPDSQTLQLLY
                     SMSLSDKPVIENYESGGSKAQLPNVDLKTINQTVDLKSGQTVIISGFQQSGRRSGKQG
                     VGTPGFFGLGGGINSENDDTILVVLITPNII"
     gene            complement(5302..5739)
                     /gene="pilM"
                     /locus_tag="PN977_004130"
     CDS             complement(5302..5739)
                     /gene="pilM"
                     /locus_tag="PN977_004130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001681791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus biogenesis protein PilM"
                     /protein_id="HDI6044108.1"
                     /translation="MGWFIVFIMVITTTGNFWFTSQLSRSEHRHQAAENTQQAATFIR
                     YMNAINDYLHQNPERRTAGGRLTSAQLGLPGTDTVSHIISQQRVFVWATETPGLMAAL
                     REQSHDSALLGRVENGRLLDTAGRALSVTLPSVIPDHVILWMN"
     gene            complement(5744..6814)
                     /locus_tag="PN977_004131"
     CDS             complement(5744..6814)
                     /locus_tag="PN977_004131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000730426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TcpQ domain-containing protein"
                     /protein_id="HDI6044109.1"
                     /translation="MQKKSLTLLLPSLLSGCTAFNATPVPDTTPPALVFVDGQIRESA
                     AIITHTQKRLLPPPVTRPVTPPRPTTTATPSTTVSSQSTALPPRPLLQATQLPALVMT
                     GKPGQSSVVYLREARQRSVSDWIRQLLPTGWQFRQQNAATPALNKRLPSWSVNDQWHR
                     ALNALLAGQQLYGHMDWNRKILTVTTSATPPVDVTAPQGSQKAADTPRNPFRGSTATP
                     AGPTPVTPAVSVSRAQTTTGTSGTPESRTTTPAPVATKPVVTPPQKVIPPRQWKMEKG
                     TTLKDGLARWTSSEKCPHGTWTLRWETPVNYSIDAPLVFTGSFRQALEQLFALYTPVE
                     KPLYARTSTAQCLLRVTDRPQE"
     gene            6936..7331
                     /locus_tag="PN977_004132"
     CDS             6936..7331
                     /locus_tag="PN977_004132"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6044110.1"
                     /translation="MIKKMWRYLRQIDKYELLQQVISVHEVELLRKMMTDSLLGCSVG
                     YVFSKILKISFLRFIQSEGGWDIITYFLWMLGMLIVMTVLFIWMCAAVCYELRMYKIK
                     TGRENDILFWVWILLFDVAVVFSLGNVIW"
     gene            complement(7836..8297)
                     /locus_tag="PN977_004133"
     CDS             complement(7836..8297)
                     /locus_tag="PN977_004133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001201714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilI type IV pilus biogenesis protein"
                     /protein_id="HDI6044111.1"
                     /translation="MIELAEATRQKGLAEAEAQRALNDAINVLSDEQTSLRFRLALLD
                     ALPAVIEKSTELFHKECPHGIFWFKRGLKMSAEKFRVVIYTNTCEKKVICIPATEDIN
                     IAIMPFQTADNALIIVCQNTRIIRRTERIPGSCSEWKEVALPLKARSRIKL"
     gene            complement(8355..9020)
                     /locus_tag="PN977_004134"
     CDS             complement(8355..9020)
                     /locus_tag="PN977_004134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000154135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="traC"
                     /protein_id="HDI6044112.1"
                     /translation="MTILSQENPVILLLENMLEALSTAPDNINNERRRRRYLLNWLDT
                     ARQMREFRGMAEEFTTLRKLLATNTDIPVANTLKSLLESGNNAALYDLYRFRSALSAC
                     RSLGWQIGTCAWNDQLLSETLSRAQTGKRHILQLNSTRDTFSTTGRMLRPVAFSLIHP
                     ASLESSEVEDIFRDEGFILAHGRECSLNGSGRNMLSRILHVGHSGLPKAEWGIDHSNH
                     LLH"
     gene            complement(9161..9802)
                     /locus_tag="PN977_004135"
     CDS             complement(9161..9802)
                     /locus_tag="PN977_004135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000335849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusG"
                     /protein_id="HDI6044113.1"
                     /translation="MCLSELQAGLNVGQPLFPTHALCIRRSQQHRSPPAGIDFRGAFR
                     ILNISELHQRNWYLAQYIPAGKNREHLFSWLSEQHVLPWTPLILKKVRRTDKVCGYRR
                     HIHAVFPGYFFLKADPERHNFTHLRRHSAFLNFVKMAGEIKTVREDIVQSLMKVYPDP
                     ALNPAAREELDAASTLWLTKARYQYLLRLDAQPLPESRIALLLHLVSDDGALT"
     gene            10503..11375
                     /gene="repA"
                     /locus_tag="PN977_004136"
     CDS             10503..11375
                     /gene="repA"
                     /locus_tag="PN977_004136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="incFII family plasmid replication initiator
                     RepA"
                     /protein_id="HDI6044114.1"
                     /translation="MAALNQTSFTESTYRQVKNPNPVFTPREGAGTLKFCEKLMEKAV
                     GFTSRFDFAAHVAYARSRGLRRRMPPVLRRRAIDALLQGLCFHYDPLANRVQCSITTL
                     AIECGLATESEAGKLSITRATRALKFLSELGLITYQTEYDPTIGCNIPTDITFTPALF
                     DSLDISEEAVASARRSRVEWENRLRKKQGMDALGMDELIARAWRFVRERFRSYQAELK
                     SHGMKRARARRDAGRTRQDIVTLVKRQLTREIAEGRFRGSLEAVKREIDRRVKERMIM
                     SRNNNYTRLATASP"
BASE COUNT         2766 a         2893 c         3358 g         2967 t
ORIGIN      
        1 aaagacgtgc atcctgaggc accccgggat caaactgtgt gcccttgata acatcggtca
       61 tgccggaata ggtggccccc agtaattcga ggccttcttc tttccggagg atatcgagca
      121 cttccagttc gccgtgaacc cgggcttcca cataggtgaa gtccgtgttg tctcgtcccg
      181 gggtcatatg aaggtctgag gcaccaagag aggcagaatg gcggatatag ccgagcattt
      241 tctgctgtac cgggttaatg acgctttcat caccggcggt tgtggtgtcc tgtctgaggc
      301 gggctatcac atccagggtg gtgaattcaa agtctcccac acgcccctga tgacgtgcgc
      361 gcaggcgggt cagatattcc tggacggcac gcagatgttt ttttccctcc acgaccctga
      421 cccgcagatg accggtttta tctgtgtcga tagccacata atccagaatg tcatccggtg
      481 gcaggtctgc aggatccaac ataaggcctc cttacgggca gcggcgcccg gaaaatatgt
      541 tttctgttat taagcgaggg aaataatcag ttcttctgcg gagagaatct ggacattttc
      601 agcacaggct taacagtgta aaaatgatta cgcctccaaa agcgaataac aggggaatgt
      661 ttttctccgg ctgtgttgat ctcagcgtta ataccggtgt ggcgagagcg taaaaaataa
      721 tacctgcggt ataaataaaa taaggtgtga tgccggacag attacataca ctggcgaata
      781 tcataagcat gacggcaact accgataaaa taatggaagt attcttccat tcaccagatg
      841 gtgtaagtga aatgatatcg ccatcctgtt tatagttcgt gttcagcttc aggaaatgac
      901 ggatatcttt cagcgtgttc agtttctgct ggatctcttc cacaagttcc tgtctgtcgg
      961 ttttttcacg gagcagctgg aattttgtct ccctcagtac agatataata cggtccacct
     1021 ggccggggga gggatagtaa tgatgttcga gcatccaccg gatatcggcg attaaccggt
     1081 cagttaccgg tgtattaccg ttgttaatga gaggtgtttc gtgcatccac tgaaatatgc
     1141 ggtagatatg ttgcttaaca aaaataaaat catcggtggt tttattctcc agcatgagat
     1201 atatcccgtc gagtgttaat aataaaacgt cccgggtgtg aggtgatttg tgatgtattg
     1261 tacggaatat catttctgca ctgtaatcgt ttacgcaggt gatatatcac accagagtaa
     1321 atacagcgga atgaagctgt agagtgacca gaaaagcccg acgggatctt ttgcccacag
     1381 gtataagaat gtcagtccgg tgcaccatgc gccggcaagt gccgcgcata attcagcgcc
     1441 tccggaattg ccgctcttca tcatataaca gagcgcgatc agcagtggga taatgaagac
     1501 gagcggcagg acaaaggatt cggctgcgga tatccattcc cggtaaagcc agcggtcgtt
     1561 taatctgtcg gcatcatatt tcagacagtt gctcaggaac gccagatcgt tcatgagggg
     1621 ggcgtcctgg ccatactgcg ccgcgaggcg cattctggca ccccacaacc gggcactcag
     1681 ggcacggagt tgtgccgggg tgacctgttt tccccgacaa agttgtacct gtaattgtgt
     1741 tgcctcgtgg acaatatctg tgtggttcac cccatattct gcagcggctg tttccaggtc
     1801 acggatgatc aggctgagcc aggatatcag ttcactgatg ttcattgtcg ttcatccttt
     1861 tgtacggaca gccgcctgta ctggcggccg gtttcatggt cgcagggttt ttctgatgcc
     1921 ggactgctcc agtaccacac tgtccggtgt gattttctcc acgagataag gggttccggg
     1981 gatgttctgg cccgtcttca catcggtgat gtgtccaccc gaaagttcta tccgggctct
     2041 cagctgaggc tgtacaccaa atatttctgt cagacggggg gatgtcgccg tcgacggggc
     2101 agtcgggatg gcggacgtgc tttcctgcgg acgaccgggc tctgcgggaa taccctgctc
     2161 cagcatgcgc agttcatgac ggattttctc tctcgccagc atggcttcat acagggctgt
     2221 ttcgtcctgt atccgctcaa tctgcagttg ccggggactc tgttcctctg cgtttccggt
     2281 tgtggccatt gccgttgccg gtaacagcag cgctgaaacc agaagggctt ttgttttagc
     2341 gtgcataata tttacctgta aattgatagt ggaaacgacc gttctccagt tgtcgtgtga
     2401 cagagataaa acggacagaa ggcgccttca gacgttcaac cagctggtat gccgggagta
     2461 gcgtctgtat ctgaaggtca tactctttcc acggaggctg gataatattt ccgttttcat
     2521 cggtgacgct gttactgacc tcctgccagt ccagcgtcag tggcattgac tgagcaagcg
     2581 tggtcagttg ttcttgcagg acagagcttt ccggcagagc atcaacgtcg acgggatgtt
     2641 caggcaggcg gaagctgact ggcaggccaa tgccgccggt ctttccttcc ggcatcacaa
     2701 taaaggggcg ggtacccgtc atatcactga tgcgccggat gaagtcagtc actgtcgcgc
     2761 cagctgtatt agtgtacgag gcgcgaagat tgccgctgcc gccgtcagtt gaacattcag
     2821 ccagcgaata acgccatccg gaggcagaca gtggcagtga cgtccatatg tcagcacatc
     2881 cctggataaa ggcgtcggtt tcggggagtc cgggccaggg gggactgatt tgttcctctg
     2941 tgacctgtcg ggcagctgct gcctgcgcgg cttgttcagt cttccaccgt tcataatact
     3001 gccagccgta ccatgcagcg ccgagaaggg cgatgatcag cgagacagtg atgaactgcg
     3061 ctccccggga taccggattc aggcgcgcgg cagccggatg tttaccagag ccggtcaggg
     3121 tggacagcgg cagggcctga ttctgatcgc ctgctgaaaa tgccaccggt tcatagcgtg
     3181 tccagccctg ctccggttcg gtgttgatgg aaagaaaggt atcccgggct tcacggacct
     3241 cttctccgct tcccaccagg tcactgacga gaacaccgtc gacagcggaa acaaagctgt
     3301 agcgcggtgt ttctccgggt aacgtcagtt ccgtgatggc atagccgcca tgttcaagca
     3361 gtggcaggat cagcagggcg agggagaagc actgacgggg ttttccgggc agtatgcggc
     3421 gactgacagt ggctaccatg gtgtgggtat cggcatcttc attactgaga tactgacagg
     3481 ccaggaaaag gtcggctttc tgtgcccggg caaagtcgcg ggcatgccgg tactgtgagg
     3541 gcagtgtttt ccattccagc ccggccagaa acacattgcg ccctgccggc agcggaaaca
     3601 cctgccgctc tgtcggtgaa atatccgtca cgatcgtcct ccttgtgaaa atcagatgat
     3661 attgggcgtg atcagcacca cgagaatggt gtcgtcgttt tcactgttga tcccaccgcc
     3721 aagaccgaag aatcccggcg tgcctacacc ctgttttccg ctgcgacgtc ctgactgctg
     3781 gaagccactg ataatgaccg tctgcccgga tttcagatcc acggtctgat tgatggtttt
     3841 cagatccaca ttcggcagct gggctttact tccaccgctc tcataattct caataaccgg
     3901 tttgtcggag agactcatgg aatagagcaa ctgcagtgtc tgtgagtccg ggagaatgaa
     3961 cggcagaagg gtcatgttaa aaccggtggt gatggtggcc gcattcaggg aggtggatga
     4021 gccgacattg gcagtggtat ccgtcgtcac actctcgatg taactctgct gattggcgat
     4081 ctgcattggc accggcgtca ggtttttggt gacggcagag tgctgggtca ccacactgac
     4141 aaccccctgg gaagacaatg ctttcaggaa ggcttttgaa ccggtgagtt taccgtccac
     4201 aatggaaaca ccacctgtga tggcatcact ggctgcactg gtgaatgtgc tgccgagact
     4261 cagtcccagc gttctgtcgc tgaagactgc gttccagtcg ataccggcct gctcattgcg
     4321 ggttttccgg atactcagaa tttccacatt cagcaccacc tgacgtttca tctcttcatt
     4381 gcgtttttca atgaatgccc ggaccgtttc cagaacctgt ggattatctg tgacggttat
     4441 aaagcccgtg gacatgaaca tgcgtccgat acccggggtc agcattgatg tgaccgcatt
     4501 tttcacatcc tgatacagcg cagacttcat atccacggtg gtggtctggg tattgctggc
     4561 atcaccggaa atggcatctg atgcactgcc tcctgatgag ccgtttgagg acatggtgcc
     4621 gctgaccaca gtggcgttat aggtcacctg actgtccata taggtgatgg ggaaggtctg
     4681 tgtctcgaga taatagaaga tgatatgcga gccgtcatag cgccaggata taccgagacg
     4741 ggaggagacc gcattcagca gggtcgtcag gccggaggca ttcagttcag acagtgctgc
     4801 ggtatttccc ggcagacggg atgatggcgt gtcagatgcg ccaatggcgg caaggggaac
     4861 catgccgctt gcatccggtg ggggtaacgc gccctggatt tgttgtgtgc tgccggttgt
     4921 ggatgtattc aggtatgtca gtacatcagg tgtgatccgt acaggaatat ggcagctttc
     4981 cgtgatccgc tgaaccacct gctgaagggt gatgctctga cgggtatgaa tgacaatggg
     5041 acaaccggga acggttttgc cggtgttttt atccatgacc ggacggggat tgatccattg
     5101 tcgagaggat tccttaacaa ccggcgtccg ggttcgcaga tcagacatca tttcccgtgc
     5161 ccgttgttgt tccgcaacgg cttcctttct tgtgctctca agctgtctgt aactggcaca
     5221 gccctgtaac agagcggcgg caatcataat ggcaagaatt tttttattca tgggagatcc
     5281 gtgagttatt cagattcgtg actaattcat ccagaggatg acgtgatcgg ggatcaccga
     5341 cggaagtgtg acggaaagcg ctctgccggc ggtgtccagc agccggccgt tttccaccct
     5401 gccgaggagg gcagagtcat gactctgttc cctgagggcg gccatcaggc ccggggtttc
     5461 tgtggcccag acaaaaacac gttgctggct gatgatatga ctgacggtat ccgtcccggg
     5521 aagcccgagc tgagcggatg tcagccgccc tccggctgtc cgtcgttccg gattctgatg
     5581 caggtaatca ttgatggcgt tcatataacg aatgaacgtt gctgcctgtt gtgtgttttc
     5641 tgcggcctga tgacggtgtt cgctgcggga gagctgactg gtgaaccaga aattgccggt
     5701 ggtcgtgatg accatgatga acacgatgaa ccagcccata aggttattcc tgtggacgat
     5761 ctgtcacccg cagcagacat tgtgctgtgc tggtccgggc atacagcggt ttttccaccg
     5821 gggtgtagag tgcaaatagc tgttccagcg cctggcggaa actgccggtg aaaaccagag
     5881 gagcatcgat gctgtagttg acaggtgttt cccagcgcag tgtccatgtg ccgtgcgggc
     5941 atttttcgga tgatgtccag cgggccagtc cgtctttcag ggtggtcccc ttttccattt
     6001 tccactgccg tggcggtatg accttctgcg gtggtgtcac taccggtttc gtggccaccg
     6061 gggctggcgt ggttgttctg ctctctggtg ttccggatgt cccggtggtg gtctgagccc
     6121 gggatacgct gacggcaggt gtcactggtg tcgggcctgc cggcgttgcg gtgctgcccc
     6181 tgaacggatt acgcggtgta tcagcagctt tctgtgaacc ctgcggtgcc gtgacgtcga
     6241 ccggcggtgt tgcagaggtc gtgacagtca gtattttcct gttccagtcc atatgtccgt
     6301 aaagctgctg gccggccagc agcgcattca gtgcccggtg ccactggtca ttaacggacc
     6361 aggaagggag gcgtttgttc agtgccggtg tggcggcatt ctgctggcgg aactgccagc
     6421 cggtcggcag caactgacgt atccagtcac tgacggagcg ctgacgtgcc tcacgtaaat
     6481 aaacgacgga tgactgaccg ggtttaccgg tcataaccag tgcgggcagc tgtgtggcct
     6541 gaaggagtgg gcggggaggc agtgcggtac tctggctgct gacagtggtt gagggtgtcg
     6601 ccgttgttgt cggacggggc ggtgtcacag ggcgggtcac cggtggcgga agcagacgtt
     6661 tttgcgtatg ggtgataatg gcagcacttt cgcgaatttg tccgtcgacg aacaccagag
     6721 ccggaggcgt ggtatccgga acaggcgtgg cattaaatgc cgtacagccg ctgagtaatg
     6781 acggcagaag gagtgtcagt gatttctttt gcatagaata taatccggta tattacgggt
     6841 tacggtataa gacaggtggc gggtaactgg ccttcagatg aatatcattt catcgggtta
     6901 ttgatgatga accttaaaaa tcacagaggt gatgcatgat aaagaagatg tggcgttatt
     6961 tacggcaaat tgataagtat gaattgctgc agcaggtgat ttcagtacat gaagtggaac
     7021 tgctcaggaa aatgatgaca gattcattat tagggtgctc agtaggatat gtgttttcga
     7081 aaatactgaa gatatccttt ctgcgtttta ttcagtctga ggggggatgg gatattatta
     7141 cttacttttt gtggatgctg ggaatgctta ttgtcatgac cgtgctgttt atctggatgt
     7201 gcgccgctgt atgctatgag cttcggatgt ataaaataaa aacaggaagg gagaacgata
     7261 tattattctg ggtgtggatt cttctttttg atgtcgcggt tgtattttcg ctgggtaatg
     7321 ttatctggtg attatgggtt cagtgacata tggaaaaaga taagtgcgaa cattgatact
     7381 gaagaataat atatccagga atagaaaaac atgaatttcg gagatggttt cttctgtctc
     7441 ctgtttttca ggggagtctg tgggtcaaat gttatcagcg tcattaaagg ataccctttc
     7501 ataagaataa aaatatagac gcataaatga gcgtaaagag gtaacctgtc ctgccagaac
     7561 agaatcatca tgatgacggc aagatgaaat gtcgttacat tgatataata aaaccagctg
     7621 tatttacgca gatactgttg gatattcacg ctgtcagcat gcggttctgg tgaacaattt
     7681 atgcaaaaac agaagaaaga aatgatgatg aacacggtca gagtgaccgg taatacagtc
     7741 acaagcagaa agagtatgga cataaaaata tctccgtcag gatagtgtga ttattccttt
     7801 atggttccat aacccggcgt agctccgtcg actggttata attttatccg ggacctggct
     7861 ttaaggggta atgccacctc tttccattca ctacagcttc cgggaatacg ttctgtgcgt
     7921 cgtattattc gtgtgttctg gcatacaata atcagggcat tgtctgccgt ctgaaaaggc
     7981 ataatcgcaa tgttaatatc ttctgttgcc gggatacaga tgactttctt ttcacatgta
     8041 ttggtgtata tgacaactct gaatttttct gcggacattt tcaatcctct tttaaaccag
     8101 aatatcccgt gagggcattc tttgtgaaaa agttctgtgg acttctctat gacggcaggt
     8161 aatgcatcca gcagggcaag acggaagcgg aggctggtct gctcatccga aagcacgtta
     8221 atggcatcgt tcagtgcgcg ctgtgcttct gcttctgcca gacctttctg acgggtggcc
     8281 tccgccagtt caataattgc tgctgcctgg agaagtcagc ggagagtggc tgccgttgtg
     8341 gctgtcccgg tatttcagtg taacaggtga ttgctgtgat ctatgcccca ttcggctttc
     8401 ggtaaacctg aatgccccac atgaagaatg cgtgacagca tgtttctgcc gctgccgttc
     8461 aggctgcatt cgcgtccgtg cgcgagtata aaaccttcat cccggaatat atcttccact
     8521 tctgaagatt cgagggatgc cggatgaatt aatgaaaatg ccaccggtct gagcattctg
     8581 cccgtggtgg aaaaggtgtc tctggtactg ttcagctgca ggatatgacg tttacctgtt
     8641 tgcgcacggg aaagtgtctc actcagcagc tggtcgttcc atgcgcacgt gccaatctgc
     8701 catcccagac ttcggcacgc ggaaagagcc gagcggaagc ggtacaggtc gtataatgct
     8761 gcgttattgc cactttccag cagtgatttc agcgtatttg ctaccggtat gtccgtatta
     8821 gtggccagca gttttcgcag agtcgtaaac tcttctgcca ttcctctgaa ctcgcgcatc
     8881 tgacgggcag tgtcaagcca gttcagcagg taacgccggc gacgccgttc gttgtttatg
     8941 ttgtccgggg cggtggagag tgcttcgagc atattctcca gtaacagaat gacagggttt
     9001 tcctgggata gaatggtcat aaaggcgtcc tgaaaaaatg cccgacagcc gctctccgta
     9061 caccgtaagg tgtcaccggt gccgggcatt gtggaaacag aagggaaaaa aatcccggcc
     9121 ataaaggccg ggaaaagtcg tcaaattaag acagcaattt tcatgtcaac gctccgtcat
     9181 cagataccag gtgcaggagc agggcgatcc tcgactccgg cagaggctgt gcatcaagcc
     9241 ggaggaggta ctggtaacgc gcttttgtca gccagagggt ggaggcagca tccagctcct
     9301 ccctggctgc cggattgagc gcaggatccg ggtacacttt cattaaagac tgaacgatat
     9361 cctccctgac tgttttgatt tcaccggcca ttttcacgaa gttcagaaag gcactgtgac
     9421 ggcgaaggtg agtgaagttg tggcgttcgg ggtctgcttt caggaaaaag taaccgggaa
     9481 acacggcgtg tatatgtcga cggtaaccac agactttatc cgtgcggcgg acttttttca
     9541 gtatcagggg tgtccaggga aggacgtgtt gttcggaaag ccaggaaaag agatgttcgc
     9601 ggtttttgcc ggcagggatg tactgagcca gataccagtt gcgttgatgt aattcactga
     9661 tattcaaaat ccggaacgct ccgcgaaaat cgatccccgc aggggggctt cgatgttgtt
     9721 gtgaacggcg aatacataaa gcatgtgtcg gaaacagcgg ctgacccacg ttcagcccgg
     9781 cctgcagttc tgacaggcac atgatttacg cactcttgta agccttgtgg acggtttcga
     9841 ggcgggcgaa aaccgacaac tcctgcaaac aggattaatg gctggaaaat atattacata
     9901 aggatgtatc tgtggcaaga gccaagataa gcagttgaat agatcgttat atttgataaa
     9961 tcagaatatt atgctgattt gtgtgcagtg tctggaggat tagatcgttt aaaaatttta
    10021 tgcgatctct tctactaaaa atgatgaaaa acacagaata aaagatcgtt tgtgaaattg
    10081 ttcatttttt gacacagttc aaaaaaacga cgctttttag ggggttaaag agggcaactg
    10141 taaccctgag aaatcgtgtc ggcttttcaa agcggtggaa aaggggtata ttgcggatcg
    10201 ttattcagtg gcttttggga tcctcgcggt ccggaaagcc agaaaacggc aggatgcgcc
    10261 ataaggcatt caggacgtat ggcagaaacg acggcagttt gccggtgccg gaaggctgaa
    10321 aaaagtttca gaaggccata aaggaaaacc cccactatct ttcgtcgaac tttggcggga
    10381 tcgtgaaaga tagtgggggc ctcacagaat acgggataag tatatatgaa accgtgtcag
    10441 agattcaacc ctttgcagtg cattaacgca cggcaatctg ttcccgccat aagcgacagc
    10501 ttgtggcggc cctgaaccag acctcattta ctgaaagcac ataccgtcag gtaaagaacc
    10561 cgaatccggt attcacaccc cgtgaaggtg ccgggacgct gaagttctgc gaaaaactga
    10621 tggaaaaggc cgtgggcttc acctcccgtt ttgattttgc cgcgcatgtg gcgtatgccc
    10681 gttcgcgcgg cctgcgccgg cgtatgccgc cggtgctgcg ccgccgggcg attgatgcgc
    10741 tgctgcaggg gctgtgtttc cactatgatc cgctggccaa ccgcgtgcag tgctccatca
    10801 ccacgctggc cattgagtgc ggactggcga cggagtctga ggccgggaaa ctctccatca
    10861 cccgtgccac ccgtgccctg aagttcctgt ctgagctggg gctgataacc taccagacgg
    10921 aatacgaccc gacaattggc tgtaacatcc cgaccgacat cacgttcacg ccggcactgt
    10981 ttgactcact cgatatctca gaagaggcag tggcttccgc acgccgcagc cgcgtggaat
    11041 gggaaaacag gctgcgcaaa aagcagggga tggatgcgct gggtatggat gaactgatag
    11101 cgagggcctg gcgttttgtg cgcgagcgtt tccgcagtta tcaggcagag cttaaatccc
    11161 acggtatgaa acgtgcccgt gcgcgccgcg atgcaggcag aacccgtcag gacattgtca
    11221 ccctggtgaa acggcagctg acacgtgaaa ttgcggaagg gcgtttccgg ggaagcctgg
    11281 aggccgtgaa gcgcgaaatc gaccgtcgcg tgaaggaacg catgataatg tcgcggaaca
    11341 acaactacac acggctggcc actgcctcac cctgagtttg acctcatctg aataatccgg
    11401 cctgcgccgg aggcttccgc acgtctgaag cccggtgacg cacaaaaaag cagcacagca
    11461 cacaaaaaac agcctcatta tctgccttcc ggctcgcctt gctccccctg tttttcacgc
    11521 aaaacaggta tcacagacgg ggaattctgt ttatccacat ttaactgcaa gggacttctc
    11581 cgtaaggtta caaccgttca tgctataaag cgccagccgc aggcgttaca gggtgcaatg
    11641 tttcttttaa accccagtac tgtatttcct ttaaactact taattacttc catttaaaaa
    11701 gaaaacctgt tcagtgcctg tcctgtggac agactgatat gcacctccca cgctaacgcg
    11761 cgggccccga acctcaccat tctgaaacca cagcaaaaaa acatcaggaa taaaaaacac
    11821 cacacaaaca cagcactgta ccccccccca taactgaaaa gcgccgccgc ccccgcccga
    11881 agggcgggaa caacatcgct tttaattatg aatgttgtaa ctacattatc atcgctgcca
    11941 gtcttctggc tggaagtcct cagtacacgc tcgtaagcgg ccct
//
LOCUS       DAMZFT010000143        11922 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.143, whole genome shotgun sequence.
ACCESSION   DAMZFT010000143 DAMZFT010000000
VERSION     DAMZFT010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11922)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11922)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11922
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.143"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(134..298)
                     /locus_tag="PN977_004137"
     CDS             complement(134..298)
                     /locus_tag="PN977_004137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015980168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2737 family protein"
                     /protein_id="HDI6044115.1"
                     /translation="MRGLAYNPGILPAEMIIRQRVKPMPSREELLKRNSFPSVYQNKY
                     LNAMWRSGKK"
     gene            complement(309..602)
                     /locus_tag="PN977_004138"
     CDS             complement(309..602)
                     /locus_tag="PN977_004138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001111277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2856 family protein"
                     /protein_id="HDI6044116.1"
                     /translation="MPAPQYGADDPRRCSGNSVSEVLDKFRKNYDLIMSLPQETKEEK
                     EFRHCIWLAEKEERERIYQTSIRPFRKATYTHFPEIDPRLRNYRSRYGAISND"
     gene            complement(616..1122)
                     /gene="ssb"
                     /locus_tag="PN977_004139"
     CDS             complement(616..1122)
                     /gene="ssb"
                     /locus_tag="PN977_004139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000168270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HDI6044117.1"
                     /translation="MSSRGINKVIILGRVGQDPEVRYSPSGTAFANLTIATSEQWRDK
                     NTGEQKELTEWHRVAVSGKLAEVVGQYVKKGDQIYFEGMLRTRKWKDQSGQDRYTTEV
                     HVGINGVMQMLGGIGDSKQQASSRQSQKPQQQSSPAQHNEPPMDFDDDIPFAPVTLPF
                     PRHAIHAI"
     gene            complement(1119..1586)
                     /locus_tag="PN977_004140"
     CDS             complement(1119..1586)
                     /locus_tag="PN977_004140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000018648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HDI6044118.1"
                     /translation="MSHLDGIIKRFESSYKVNETTGCWESTYSKNKGGYTKFVAFGVT
                     MLSHRVAFELYHSPIPSGKMVCHKCDNPCCVNPEHLFLGSAQENMDDKIAKGRHRGAK
                     KGHAHHGAKLTEWQVIEIRKRLSEKESQYKIAKDMGVSQSIISNIKTGKRWSK"
     gene            complement(1587..2294)
                     /locus_tag="PN977_004141"
     CDS             complement(1587..2294)
                     /locus_tag="PN977_004141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
                     /protein_id="HDI6044119.1"
                     /translation="MDLNKFDEPFSPEDIEWRIQQSGKTRDGKVWAMVLAYVTNRAIM
                     KRLDDVCGKAGWRNEYRDIPNNGGVECGISIKIDSEWVTKWDAAENTQVEAVKGGRSG
                     AMKRAAVQWGIGRYLYNLEEGFAQTSLDKKQGWHRAKLKDGTGFYWLPPSLPGWAIPA
                     SDNKPSPENTNQKSPSVDCEQILKDFSDYASTETDKKKLIERYQRDWQLMAGNEEAQA
                     KCVQVMNIRVNELKQAA"
     gene            complement(2549..2719)
                     /locus_tag="PN977_004142"
     CDS             complement(2549..2719)
                     /locus_tag="PN977_004142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001183768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6044120.1"
                     /translation="MQQFAIAGAASVRPFNPILSVQHSRKNILTGADFKQPRMKSLLE
                     KLWDILKQQGRP"
     gene            complement(2959..3147)
                     /locus_tag="PN977_004143"
     CDS             complement(2959..3147)
                     /locus_tag="PN977_004143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007829703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6044121.1"
                     /translation="MKRKFEIWLDSGANIHSCKKTVVTLDDLGLSENEWNEMDQDEKD
                     ENMRDVAFETLDWGWKEI"
     gene            complement(3207..3458)
                     /locus_tag="PN977_004144"
     CDS             complement(3207..3458)
                     /locus_tag="PN977_004144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6044122.1"
                     /translation="MEFHESAICDFRANANSVKPQPIAVLFKTMGAWAVLCFASDDTD
                     ARMAIGQEMEMDPTNDEFIIYGAPSNYLLDTCNIYNKAA"
     gene            complement(3467..3799)
                     /locus_tag="PN977_004145"
     CDS             complement(3467..3799)
                     /locus_tag="PN977_004145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001515059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HDI6044123.1"
                     /translation="MDAQTRRRERRAEKQAQWKAANPLLVGVSAKPVNRPILSLNRKP
                     KSRVESALNPIDLTVLAEYYEQIESNLQRIERKNQRTWYSKPRSEMGVTCSGRQKQRG
                     KSIPAYYD"
     gene            complement(4190..4594)
                     /locus_tag="PN977_004146"
     CDS             complement(4190..4594)
                     /locus_tag="PN977_004146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000618033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6044124.1"
                     /translation="MIRIAALLSILLTASANSECWIVTNLHGYGAMNGDRYGFTKDST
                     EDSVFHVTINGDKSSVYESVSGVYPEMKYTALSSNTMVGEYQSGGGITVETWSITTDK
                     KALYSKVMNIPGMQQLTSTKSFVGDVVGTCNQ"
     gene            complement(4591..5223)
                     /locus_tag="PN977_004147"
     CDS             complement(4591..5223)
                     /locus_tag="PN977_004147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000028393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family transcriptional regulator"
                     /protein_id="HDI6044125.1"
                     /translation="MSISSRVKSKRIQLGLNQAELAQKVGTTQQSIEQLENGKTKRPR
                     FLPELASALGVSVDWLLNGTSDSNVRFVGHVEPKGKYPLISMVRAGSWCEACEPYDIK
                     DIDEWYDSDVNLLGDGFWLKVEGDSMTSPVGQSIPEGHMVLVDTGREPVNGSLVVAKL
                     TDANEATFKKLVIDGGQKYLKGLNPSWPMTPINGNCKIIGVVVEARVKFV"
     gene            5327..5542
                     /locus_tag="PN977_004148"
     CDS             5327..5542
                     /locus_tag="PN977_004148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001194218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDI6044126.1"
                     /translation="MQTLSERLKKRRIALKMTQTELATKAGVKQQSIQLIEAGVTKRP
                     RFLFEIAMALNCDPVWLQYGTKRGKAA"
     gene            5662..5940
                     /locus_tag="PN977_004149"
     CDS             5662..5940
                     /locus_tag="PN977_004149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001177654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lambda phage CII family protein"
                     /protein_id="HDI6044127.1"
                     /translation="MQLTSTRKKANAITSNILNRIAVRGQRKVADALGINESQISRWK
                     DSFIPKMGMLLAVLEWGVEDEELAELAKKVARMLTKEKAPKNGEFFEA"
     gene            5975..6121
                     /locus_tag="PN977_004150"
     CDS             5975..6121
                     /locus_tag="PN977_004150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2740 family protein"
                     /protein_id="HDI6044128.1"
                     /translation="MTKQLSPYQDKIHKHILRDRFLSSFKQPGRFRAELEKVKLMQKE
                     KGHE"
     gene            6114..6974
                     /locus_tag="PN977_004151"
     CDS             6114..6974
                     /locus_tag="PN977_004151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016234696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HDI6044129.1"
                     /translation="MSNLATVTHLRPSQRPVERRVAEVEDGYTRLANALYEELIGADL
                     TKNQSKVAHAICRKTYGYGKKMDRISDSQLAQITRLPRQKVNKAKNELIAMKVILREG
                     QQIGPNKNIEEWQIEGCHYSGDNVTALVTKSVTKMVTALSPKQGHTKETITKEKRNNK
                     NTMSESVRTECEKSPDRHEETDKAFEEIFWCAGMRKAGKKNAASAFRTQFREWRKTTR
                     GTASEFATMLAEDIACRNGKQFGFDRLLPSSYLNGQRWNDEKPETIQPQSKPSSAITV
                     SKTGYVFFDR"
     gene            7082..8962
                     /locus_tag="PN977_004152"
     CDS             7082..8962
                     /locus_tag="PN977_004152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001322819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HDI6044130.1"
                     /translation="MTPSELSDLLWAQVDRVAPHLLPNGKKEGHEWVAGNVNGDKGNS
                     LKVNLSGKKKWADFAEGDGGDMLDLWMACRGINLHQAMQEAKAFLGIKDDDHHFDAKR
                     EKKFSRPDRKKIARYVTRTESHLEYLQSRGISPEIVKRYEVVSGKVWNGERELDALVI
                     PYKRDGELLQVKRISTERPDGKKVIMAEGDCEPCLFGWQALDAGVRAVVLCEGEIDCM
                     SYAQYGISALSVPFGGGKGAKQQWIEFEYHNLDRFEEIFISMDVDDVGREAAREIASR
                     LGEHRCRLVTLPYKDINECLMNGVTEDEIWQYIGTASYFDPEELYSAREFYQDTINAF
                     YGKQQYLFNPPWESLADKFQFREAELTLVNGVNGHGKTEVVGHMALEAMRQGVKTCIA
                     SLELKPGILLKRLTRQATCCKMPPVLEIDSAFKFYDERLWVFGLTGTAKADRLIEIFD
                     YARRRYGIQLFIIDSLMKCGIGDDDYNGQKAFVDSICDFKNKTNSHVILVTHSRKGDS
                     EEKPTGKMDVKGSGAITDLTDNLFIIWRNKARERALQRVQSGEKMSEKDEQLLASPAS
                     VLMLEKQRNGEGWEGGVPLFLDEQSHQFLQLESGSPYSYIANMPKSEYDEAWRQENVT
                     EY"
     gene            9241..9681
                     /locus_tag="PN977_004153"
     CDS             9241..9681
                     /locus_tag="PN977_004153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001513013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein NinB"
                     /protein_id="HDI6044131.1"
                     /translation="MKKLTFEIRSPAHQQNAIHAVQQILPDPTKPIVVTIQERNRSLD
                     QNRKLWACLGDVSRQVEWHGRWLDAESWKCVFTAALKQQDVVPNLAGNGFVVIGQSTS
                     RMRVSEFAELLELIQAFGTERGVKWSDEARLALEWKARWGDRAA"
     gene            9678..10550
                     /locus_tag="PN977_004154"
     CDS             9678..10550
                     /locus_tag="PN977_004154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000611491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase family
                     protein"
                     /protein_id="HDI6044132.1"
                     /translation="MINVVSFSGGRTSAYLLWLMEQKRQAGEDVHYVFMDTGCEHPMT
                     YRFIREVVKFWDIPLTVLQVDINPELGQSNGYTVWEPKDIQTKMPVLKPFIDMVKKYG
                     TPYVGGAFCTDRLKLVPFTKYCDDHFGRGNYTTWIGIRADEPKRLKPKPGIRYLAELS
                     DFEKEDILAWWKQQPFDLQIPEHLGNCIFCIKKSTQKIGLACKDEEGLQRVFNEVITG
                     SHVRDGHRETPKEIMYRGRMSLDGIAKMYSENDYQALYQDMVRAKRFDTGSCSESCEI
                     FGGQLYFDFGREAA"
     gene            10547..10720
                     /locus_tag="PN977_004155"
     CDS             10547..10720
                     /locus_tag="PN977_004155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000679699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein ninD"
                     /protein_id="HDI6044133.1"
                     /translation="MMRCYRCGECKEDNRFRPNQPDWNRWCLRRERTPTGVLPLPQEK
                     EDVWRDSDEVSPT"
     gene            10687..10863
                     /locus_tag="PN977_004156"
     CDS             10687..10863
                     /locus_tag="PN977_004156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NinE family protein"
                     /protein_id="HDI6044134.1"
                     /translation="MARQRRSITDIICENCKYLPTKRSRNKRKPIPKESDVKTFNYTA
                     HLWDIRWLRERARK"
     gene            10866..11225
                     /locus_tag="PN977_004157"
     CDS             10866..11225
                     /locus_tag="PN977_004157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000386647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2591 family protein"
                     /protein_id="HDI6044135.1"
                     /translation="MDYSQLSDFEINVAVFESIHNGSPDYKEGENGDMVFVSFEGDIV
                     NGDAVEVEVECGSFNPCVNPADAWPIIEKYRISIINLDEDEWGARGVAYCKSKRAIHE
                     NPLRAAMIVFLMMQRIQ"
     gene            11225..11401
                     /locus_tag="PN977_004158"
     CDS             11225..11401
                     /locus_tag="PN977_004158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001555369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein ninF"
                     /protein_id="HDI6044136.1"
                     /translation="MLSPSQSLQYQKESVERALTCANCGQKLHVLEVHVCEHCCAELM
                     SDPNSSMYEEEDDE"
     gene            11394..>11922
                     /locus_tag="PN977_004159"
     CDS             11394..>11922
                     /locus_tag="PN977_004159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HDI6044137.1"
                     /translation="MNELINGNAIKMTSIEIAELVGKRHDNVKRTIETLAKNGVIRLP
                     QIEDCGRINGLGLNQSFCVYVFEGEQGKRDSIVVVAQLSPEFTARLVDRWRELEEAAV
                     NIPKTLPEALRLAADLAEQKMQLENQLAIAAPKVEFADRVGEASGILIGNFAKVVGIG
                     PNKLFAWMRDHKILIA"
BASE COUNT         3241 a         2674 c         2849 g         3158 t
ORIGIN      
        1 agcgccgcgc acttggcctc cgcttcagca aatttacgca ccagataatc agcgtttgtt
       61 tcgttaacct ttaaatcact tgggatgcat ttacctttca gaaatccatc catctcaatt
      121 agtgacattt gtttcatttc ttcccactcc gccacattgc attcagatat ttgttttgat
      181 acactgatgg aaaagaattt ctcttaagca attcctctct cgatggcatt ggctttacgc
      241 gttggcgaat aatcatttct gccggaagaa tgccgggatt gtatgcaagt cctctcatgg
      301 taaattcctc agtcattact gatagcgcca tagcgtgagc ggtaattacg caggcgcggg
      361 tcaatttcag ggaagtgggt atatgtggct ttgcggaatg gtcggattga tgtctggtaa
      421 attcgctcgc gttcttcttt ctctgcaagc catatacaat ggcgaaattc cttttcctct
      481 ttcgtttcct gcggtagcga cattatcagg tcgtagtttt ttctgaattt atccagcacc
      541 tccgatacgg aattgccgga acagcggcgc gggtcatccg caccatactg aggcgctggc
      601 atgattttct cctgattaaa ttgcgtgaat agcgtgacga gggaagggga gagttactgg
      661 tgcaaagggt atatcgtcgt caaaatccat cggaggttcg ttgtgttgtg ctggtgatga
      721 ttgctgctgt ggcttctgtg attgcctgct ggatgcttgt tgtttgctgt cgccaatgcc
      781 gccaagcatt tgcatcacac cattaattcc gacatgaacc tcggttgtgt aacggtcttg
      841 ccctgactgg tctttccact ttctggttct cagcattccc tcgaaataaa tctgatcacc
      901 ttttttcaca tactgcccca cgacctcagc cagtttcccg gatacagcaa cacgatgcca
      961 ttcagtcaat tccttttgct cgccagtatt tttatctcgc cattgttctg acgtggctat
     1021 tgtcaggtta gcgaacgctg ttcctgatgg tgagtatcga acttccgggt cttgtcctac
     1081 ccgaccaaga ataatcacct tatttatccc gcgagaactc atttgctcca cctcttgcca
     1141 gtttttatgt tgcttataat tgattgagat acacccatgt cttttgctat cttgtactga
     1201 ctctcttttt cagagagtct tttcctaatt tctataacct gccactctgt taattttgca
     1261 ccatgatgag catgaccttt cttggctcca cgatgccttc cttttgctat cttgtcgtcc
     1321 atgttttctt gcgcgctacc taaaaagaga tgttcaggat taacgcagca tgggttatcg
     1381 catttgtggc aaaccatctt cccagatgga atgggggagt gataaagctc aaaagcaacc
     1441 cgatgagaaa gcattgttac gccaaaggct acaaattttg tgtatcctcc tttgtttttt
     1501 gaataggtag actcccagca acctgttgtt tcattaactt tgtagctgga ctcgaatctt
     1561 ttaataattc cgtccaagtg agacatttat gccgcctgtt ttagttcgtt aactctgatg
     1621 ttcattacct gaacgcattt agcctgcgcc tcctcgttgc cagccattaa ttgccagtca
     1681 cgctgataac gctcgatgag ttttttcttg tcagtttctg ttgacgcata atcgctgaag
     1741 tctttcagga tttgttcgca gtcaaccgat ggagatttct ggttggtatt ttctggtgat
     1801 ggtttgttat ctgatgctgg gattgcccat cccggcagcg atggagggag ccagtaaaat
     1861 cctgttccat ccttgagttt tgccctgtgc catccctgct ttttatcgag agatgtttgt
     1921 gcaaaacctt cctcaaggtt atacagatac cgaccgattc cccactgaac ggcagcgcgc
     1981 ttcattgcac cggaacgacc acctttgacg gcttctacct gcgtgttttc agcggcatcc
     2041 catttggtta cccattcgga atcaatcttg attgatatgc cgcattcaac tccgccgttg
     2101 ttgggaatat cgcggtattc attgcgccat cctgctttgc cgcaaacatc gtccaggcgt
     2161 ttcatgattg cccggttcgt gacataagcc agcaccatag cccacacttt gccatcgcgt
     2221 gttttaccgc tttgctgtat tcgccattcg atatcttcag ggctgaatgg ctcatcgaat
     2281 ttattcaaat ccataaatca cctcagaatg gacatggccc aaggaaataa cgctgattta
     2341 atacttcgac tcgggacaaa ttaaggcata cccgcattcc ttcgcggtcg ccattatggc
     2401 gataccagag agctttctgc gtgtacatgc gtctctgtaa cttgctctcc ttcactgtgg
     2461 ttgcaagtga catgaatatc tccttcgtta ccgattaatt ctttcatctg acgaatgaat
     2521 tcttcgtctg accagttatc cgtaaaactc atggacggcc ttgttgtttc aaaatatccc
     2581 aaagcttttc gagcaaactt ttcattcttg gttgtttaaa gtctgctccg gttaaaatat
     2641 tttttcgtga atgctgtacc gataaaatcg ggttgaaagg gcgaaccgat gccgcccctg
     2701 caatagcgaa ctgttgcata ggatgctcct tctgtttgat tgcataacga aaacgcctcg
     2761 agtgaagcgt tattggtatg catataaaaa agccctcaca tcggagggca aagaagattt
     2821 ccaataatca gaacaagtcg gctcctgttt agttacgagc gacattgctc cgtgtattca
     2881 ctcgttggaa tgaatacaca gtgctgtgtt tattctgttg tttatgccaa aaataaaggc
     2941 cgactatgcg gccatcactt aaatttcctt ccatccccaa tcaagcgttt caaatgctac
     3001 atcacgcatg ttttcgtctt tttcgtcctg atccatttca ttccactcat tctcggataa
     3061 gccaagatca tcgagtgtta caactgtctt tttgcaagag tgaatgtttg ctccggaatc
     3121 caaccaaatt tcaaatttac gtttcatata tcaccttgat tgtaataatc atgaaattat
     3181 ttacggcaaa aaaataaagg ccaccatcag gcagccttgt tgtaaatgtt gcaggtatca
     3241 agtaagtaat tagatggagc gccataaatt atgaattcat cgtttgtcgg gtccatctcc
     3301 atctcttggc ctattgccat tcttgcatca gtgtcgtcag aggcgaagca taaaacagcc
     3361 cacgctccca ttgttttaaa aagaactgca attggctgtg gttttactga atttgcgtta
     3421 gcgcgaaaat cacaaatcgc actttcatga aattccatat ctcacctcaa tcgtaataag
     3481 ccggaattga ttttccgcgt tgcttctggc ggcctgagca tgtcacaccc atttcactcc
     3541 gtggcttgct gtaccatgtg cgctgattct tgcgctcaat acgttgcagg ttgctttcaa
     3601 tctgttcgta gtattcagcc agcaccgtaa ggtctatcgg attcagtgcg ctttctactc
     3661 gtgatttcgg tttgcgattc agcgagagaa tagggcggtt aactggtttt gcgcttaccc
     3721 caaccaacag gggatttgct gctttccatt gagcctgttt ctctgcgcga cgttcgcggc
     3781 ggcgtgtttg tgcatccatc tggattctcc tgtcagttag ctttggtgat tggatggccg
     3841 gcgctgaacc ccggcttact ggttagagcg cccgcactac cagtgacgct gtcttgaggc
     3901 gcagattggt tactgcttgc catgagcgct gtttatacat tggtcgagca tcagcctgct
     3961 cattcatcca atcccaaagc cttctgcttt gaatgctgcc cttcttcagg gcttaatttt
     4021 taagagcctc accttcatgg tggtcagtgc gtcctgctga tggcttaaaa ttacaagaaa
     4081 gattgtatgt tgtaaacaag aaatattgta aaaaggggcg tgaaaaacaa actccattgt
     4141 ttttaaatga aaaatagttt gtttttggtt atcgagattg aggtggggat tactggttgc
     4201 aggttccgac tacatcacca acaaatgatt tggttgatgt aagttgttgc atacctggga
     4261 tattcattac tttggagtaa agagcttttt tgtctgtagt gattgaccaa gtttcaacgg
     4321 ttattcctcc tccagactgg tattctccta ccatagtgtt cgatgacaaa gcagtgtatt
     4381 tcatctctgg atagacgcca gagactgatt cataaactga tgatttatcg ccatttattg
     4441 ttacgtgaaa aacggaatct tccgtgctgt cttttgtaaa cccgtaacga tcgccattca
     4501 ttgccccgta cccgtgcagg tttgtgacaa tccagcattc agaattggcg ctggcagtta
     4561 agagtattga gagtagcgcc gcaatcctga tcatacgaat ttgaccctcg cctctacgac
     4621 aacaccgata atcttgcagt tcccgttgat gggagtcata ggccatgaag gattcaggcc
     4681 tttcaggtac ttctgaccgc catctataac cagtttcttg aatgttgctt cgttcgcgtc
     4741 agtcagtttg gctacaacaa ggcttccatt cactggctcg cgtccagtat ctactaacac
     4801 catatgacct tcagggatgc tttgccctac aggtgaagtc atggaatcac cttcaacctt
     4861 cagccagaat ccatcgccta ataagttaac gtcactgtca taccattcat caatgtcctt
     4921 gatatcgtag ggctcacaag cttcacacca cgaaccagct ctaaccatgc taatcaatgg
     4981 atatttccct ttgggctcaa catgcccaac aaatctaaca tttgaatcag aggtgccatt
     5041 gagcagccag tcaacactta cgccaagagc tgacgcaagt tctggtaaaa agcgtggtcg
     5101 cttagtttta ccgttttcga gttgctctat agactgctgg gtagtcccca ccttttgagc
     5161 aagttcagcc tggttaagtc caagctgaat tcttttgctt tttaccctgg aagaaatact
     5221 cataagccac ctctgttatt tacctccaat ctttacaaga aaaactgtat ttgacaaaca
     5281 agatacattg tatgaaaata caagaaagtt tgttgatgga ggcgatatgc aaactctttc
     5341 tgaacgcctc aagaagaggc gaattgcgtt aaaaatgacg caaaccgaac tggcaaccaa
     5401 agccggtgtt aaacagcaat caattcaact gattgaagct ggagtaacta agcgaccgcg
     5461 cttcttgttt gagattgcta tggcgcttaa ctgtgatccg gtttggttac agtacggaac
     5521 taaacgcggt aaagccgctt aagacattcc cgctcttaca catcccagcc ctgaaaaagg
     5581 gcatcaaatt aaaccacacc tatggtgtat gcatttattt gcatacattc aatcaattgt
     5641 tatctaagga aatacttaca tatgcaactt acaagtactc gcaagaaagc gaatgcaatt
     5701 acaagcaaca tcctaaatcg aattgctgta cgtggtcagc gaaaggtggc cgacgcgtta
     5761 gggattaatg aatcgcaaat ttcacgatgg aaagacagct tcatcccaaa aatgggaatg
     5821 cttctggctg ttcttgaatg gggtgttgaa gacgaggagt tggcggaact ggctaagaaa
     5881 gtagccagaa tgctgacaaa agaaaaagcc ccgaagaacg gcgaattctt cgaggcctga
     5941 tgtagaaaga ctggatcaat ccacaggagt aattatgaca aaacaactca gtccttacca
     6001 ggacaaaatt cacaaacaca tactacgtga tcgcttcctg tccagcttca agcagcctgg
     6061 tcgattccgg gctgagttgg aaaaagtgaa gctgatgcag aaggagaaag gtcatgagta
     6121 atcttgcaac cgtaacacat ttaaggcctt cacaacggcc tgtggagcgt cgtgtggcag
     6181 aagttgaaga tggttatacc cgtcttgcaa atgccctgta tgaagagctt atcggcgcag
     6241 atttaacgaa aaatcagagc aaggttgccc acgccatatg ccgtaaaaca tacggctacg
     6301 gtaaaaagat ggatcgcatc tctgatagtc agttagctca aattaccagg ctgccaagac
     6361 agaaggtaaa caaggccaag aatgagctta tcgcgatgaa ggttatcctt cgcgaaggcc
     6421 agcaaatcgg gcctaacaag aacatcgagg aatggcaaat cgaagggtgt cactactctg
     6481 gtgataatgt cactgcattg gtgacaaaaa gtgtcaccaa aatggtgaca gcgctgtcac
     6541 caaaacaggg acacacaaaa gaaactatta caaaagaaaa aagaaataat aaaaacacta
     6601 tgtccgaaag tgttcggacg gagtgtgaaa aatcacctga ccgtcacgaa gaaaccgaca
     6661 aggcattcga ggaaatattc tggtgtgcag gcatgcggaa agccgggaag aaaaacgcag
     6721 cttcggcatt cagaacacag ttcagggaat ggcgtaaaac taccaggggt acggcaagcg
     6781 agtttgccac gatgttggca gaagacatcg catgcaggaa tggtaagcag ttcggattcg
     6841 acaggttgtt accatcgagc tacctgaacg gtcaacgctg gaacgacgaa aagccagaaa
     6901 ccattcaacc acaatccaaa ccatcatccg caatcaccgt atcgaaaact ggctacgtgt
     6961 ttttcgacag gtgaaccatg aaatcaaaaa tcaaatcgct actggtcgct ggttataacc
     7021 acggctggtt aagtatttca tttgtcgatt tctggtttaa aaatctcaat ctgagggaat
     7081 catgacgcca agtgaactta gcgacctgct ttgggcgcag gttgacaggg tggctccgca
     7141 cctgttgcca aacggcaaga aagaggggca tgagtgggtt gccggtaacg tcaacggtga
     7201 caagggaaac agccttaagg tcaatcttag cggcaagaaa aaatgggctg atttcgctga
     7261 gggagacggc ggtgacatgc ttgatttgtg gatggcatgt cgtggaatta acctgcatca
     7321 ggctatgcag gaagcgaaag catttctcgg tatcaaggat gacgatcacc atttcgatgc
     7381 caaacgtgag aagaaattct ccagacctga tcgcaagaaa atagcccgct acgttaccag
     7441 aacagaatcc catcttgagt acctgcaatc gcgtggcata tcgccagaaa tcgtaaagcg
     7501 atacgaggtt gtcagcggca aggtgtggaa tggagagcgg gaactggatg ctctggtgat
     7561 tccgtacaaa cgcgatggtg agttgttgca ggtcaagcga atcagcactg agcgcccgga
     7621 cgggaagaaa gtcattatgg cagaaggtga ttgcgaacct tgtctgttcg gatggcaggc
     7681 tctggacgct ggcgtgaggg ctgttgtact ttgcgaaggc gaaattgact gtatgagcta
     7741 tgcgcaatac ggcatctcgg cgttatccgt gccgtttggt ggcgggaaag gcgctaagca
     7801 gcagtggatt gagtttgagt atcacaacct cgacaggttt gaggaaatat tcatctcgat
     7861 ggacgttgat gatgttggtc gtgaagccgc aagggaaatc gcaagccgac tcggtgaaca
     7921 tcgttgccgt cttgttactc tgccgtacaa agacatcaac gaatgcctga tgaacggtgt
     7981 taccgaggat gaaatctggc agtacattgg cacggcatcc tacttcgatc ctgaagaact
     8041 ctacagcgcg cgagagtttt accaggacac tatcaacgct ttctacggca agcagcagta
     8101 tctgtttaat ccaccgtggg aatctctggc agataaattc cagttccgtg aggccgagtt
     8161 gacgctggtc aatggtgtga acggtcacgg aaaaacggag gttgtcgggc atatggcact
     8221 tgaggcaatg cgtcagggtg tgaagacgtg catcgcgtca cttgagctga agcctggtat
     8281 tctccttaag cgccttaccc gtcaggcaac gtgctgcaag atgccgccag tgctggaaat
     8341 tgactctgca tttaaatttt atgacgaaag actctgggtg tttggcctga ccggaacggc
     8401 gaaagccgac aggctgatcg aaatattcga ctacgctcgc cgccgatacg ggatccagtt
     8461 attcatcatc gacagcctga tgaaatgtgg cataggcgac gatgactata acgggcagaa
     8521 ggcgtttgtt gactcgattt gcgacttcaa aaacaaaaca aactcccacg tcattctcgt
     8581 tactcactcg cgaaaaggag acagcgaaga aaaaccaacc gggaaaatgg acgtaaaagg
     8641 ctctggagcg ataacagacc tgacagacaa ccttttcatc atctggcgta acaaggctcg
     8701 cgagagagcg ttacagagag ttcagagtgg tgaaaagatg tcagagaagg acgaacagct
     8761 actggcatct ccggcatctg ttttgatgct tgaaaaacaa cgtaacggcg aaggttggga
     8821 aggtggtgtc ccgttgttcc ttgacgagca atcgcaccag ttcctgcaac ttgaatcagg
     8881 atcgccatat agctacatcg ccaatatgcc gaaatcggaa tatgacgagg cgtggcgaca
     8941 ggaaaacgtg acggagtatt aaatgaccat ctacatcact gggctaataa caggggctat
     9001 ttacacagta gccctttttt attggattaa gaactagggg gatcctaatg gacaccgtta
     9061 acggaatgtg ttcagacgca ccgcgtgcca aaaaatgtaa atgcggaaaa tcaccgacaa
     9121 tattcgacat ggagaacggg tgccaaatct actgcgctaa ccacgctgct gtggcggccg
     9181 cgaattatcg cagtgcggta acggagtgga ataacctgaa atctgttaga gagggaagtc
     9241 atgaaaaaac tgacctttga aattcgatct ccagcacatc agcaaaacgc tattcacgca
     9301 gtacagcaaa tccttccaga cccaaccaaa ccaatcgtag taaccattca ggaacgtaac
     9361 cgcagcttag accaaaatcg gaaactttgg gcttgccttg gtgacgtctc gcgtcaggtt
     9421 gaatggcatg gtcgctggct ggatgcagaa agctggaagt gcgtttttac agcagcatta
     9481 aagcagcagg acgttgttcc taaccttgcc gggaatggct ttgtggtaat aggccagtca
     9541 accagcagga tgcgtgtaag cgaatttgcg gagctattag agcttataca ggcatttggt
     9601 acagagcgtg gcgttaagtg gtcagacgaa gcgcgactgg ctctggagtg gaaagcaaga
     9661 tggggagaca gggctgcatg ataaatgtcg ttagtttctc cggtggcagg acgtcagcat
     9721 atttgctctg gctaatggag caaaagcgac aggcaggtga agacgtgcat tacgttttca
     9781 tggatacagg ttgtgaacac ccgatgacat atcggtttat ccgcgaagtt gtgaagttct
     9841 gggatatacc gctcactgta ttgcaggttg atatcaaccc agagcttgga cagtcaaatg
     9901 gttatacggt atgggaacca aaggatattc agacgaaaat gcctgttctg aagccattta
     9961 tcgatatggt aaagaaatat ggcactccat acgtcggcgg cgcgttctgc actgacagat
    10021 taaaactcgt tcccttcacc aaatactgtg atgaccattt cgggcgaggg aattacacca
    10081 cgtggattgg catcagagct gatgaaccga agcggctaaa gccaaagcct ggaatcagat
    10141 atcttgctga actgtcagac tttgagaagg aagatatcct cgcatggtgg aagcaacaac
    10201 cattcgattt gcaaataccg gaacatctcg gtaactgcat attctgcatt aaaaaatcaa
    10261 cgcaaaaaat cggacttgcc tgtaaagatg aggagggatt gcagcgtgtt tttaatgagg
    10321 tcatcacggg atcgcatgtg cgtgacggac atcgggaaac accaaaggag attatgtacc
    10381 gaggaagaat gtcgctggat ggtatcgcga aaatgtattc agaaaatgat tatcaagccc
    10441 tgtatcagga catggtacga gctaaaagat tcgataccgg ctcttgttct gagtcatgcg
    10501 aaatatttgg agggcagctt tatttcgact tcgggaggga agctgcatga tgcgatgtta
    10561 tcggtgcggt gaatgcaaag aagataaccg cttccgacca aatcaacctg actggaatcg
    10621 atggtgtctc cggcgtgaaa gaacaccaac aggggtgtta ccactaccgc aggaaaagga
    10681 ggacgtgtgg cgagacagcg acgaagtatc accgacataa tctgcgaaaa ctgcaaatac
    10741 cttccaacga aacgctccag aaataaacgc aagccaatcc caaaagaatc tgacgtaaaa
    10801 accttcaatt acacggctca cctgtgggat atccggtggc ttagagaacg cgcgaggaaa
    10861 tgacaatgga ttattcacag ttaagtgatt ttgaaattaa cgtggcggta ttcgaatcca
    10921 ttcataacgg atcaccggat tacaaagaag gtgagaatgg cgatatggtg tttgtctcat
    10981 ttgagggaga cattgtaaac ggagacgcag ttgaagtaga agttgagtgc ggatcattta
    11041 acccatgcgt aaacccagca gacgcatggc cgattattga aaaatacagg attagcatta
    11101 tcaatctcga tgaagacgag tggggtgcac gcggtgtggc ctactgtaaa tctaagcgag
    11161 ctatacatga aaatcccctc cgcgccgcca tgattgtctt tctcatgatg cagagaatcc
    11221 aataatgctt agcccatccc aatcccttca ataccagaaa gaaagcgtcg agcgagcttt
    11281 aacgtgcgct aactgcggtc agaagctgca tgtgctggaa gttcacgtgt gtgagcactg
    11341 ctgcgcagaa ctgatgagcg atccgaatag ctcaatgtac gaggaagaag acgatgaatg
    11401 agttaataaa tggcaatgcc ataaaaatga caagcattga aatcgctgag ttggttggta
    11461 agcgtcatga caatgtgaaa cgtaccatcg aaacgctggc taaaaatggt gttatccggc
    11521 ttcctcaaat tgaggattgt ggaagaatca atgggttagg cttaaatcaa agtttttgtg
    11581 tgtatgtatt cgaaggcgaa caaggaaagc gagacagtat tgtcgttgta gcccagttgt
    11641 cgccggaatt cacggctcgc cttgttgacc gttggcgaga gcttgaagaa gctgcggtta
    11701 atatcccaaa aacgctacca gaagcgttgc gccttgctgc tgaccttgct gagcagaaaa
    11761 tgcaactgga aaaccagctc gcaattgccg cacctaaagt tgagtttgcc gatcgcgttg
    11821 gcgaggccag cggaattttg attggaaact ttgcaaaggt tgttggaatt ggtccaaaca
    11881 aactgtttgc gtggatgcgc gatcacaaaa tccttattgc tt
//
LOCUS       DAMZFT010000144        11745 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.144, whole genome shotgun sequence.
ACCESSION   DAMZFT010000144 DAMZFT010000000
VERSION     DAMZFT010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11745)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11745)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11745
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.144"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(<1..535)
                     /locus_tag="PN977_004160"
     CDS             complement(<1..535)
                     /locus_tag="PN977_004160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDI6044138.1"
                     /translation="MKENKIPVSQEIKKHLLTGISWMIPLIVAAGICIALGQVLGGTD
                     VGEKTGTIPWMLNQIGGWGMGLIVPLISAAIAYSIADRPGFAPGLIVGFLCGQIHTGF
                     IGGMLGGFLVGYTILLLKRYIRLPQSMQGLMPIMVLPVLSTIIGGLLMMTLIGKPIAW
                     LQEALIHLLESMQGGSRF"
     gene            complement(560..874)
                     /locus_tag="PN977_004161"
     CDS             complement(560..874)
                     /locus_tag="PN977_004161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001028073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HDI6044139.1"
                     /translation="MNIVCVAACTAGIAHTYIAREKLIKGAKALGHTIKVETQGTIGT
                     ENELQAADISAADVVILAVDVKIKGEERFTNKRIVRVKTEIVIKSPVQFLEKVEKSLG
                     NK"
     gene            complement(892..1362)
                     /locus_tag="PN977_004162"
     CDS             complement(892..1362)
                     /locus_tag="PN977_004162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007370547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDI6044140.1"
                     /translation="MDITKILNTNRVILDMQATNKAEAIEELTNLLKKDGAIDCRETF
                     IKDVWQREAEGSTGFENHIAIPHGKSSAVINTTLAIGRTRQDIPWETLDGSSVRCIIL
                     FAVRLEDQNTTHIRLLSQVAGALADDDIIEQLLVETSPQKIIDLFSQYAQSDVC"
     gene            complement(1389..2942)
                     /locus_tag="PN977_004163"
     CDS             complement(1389..2942)
                     /locus_tag="PN977_004163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HDI6044141.1"
                     /translation="MQMITSRQNRLLRFLLPRREYTTIVTIAGYLNVSEKTIQRDLRL
                     LEQWLGQWRINVEKRAGAGVMLSAENIADLLHLDHLLGAECEEIDGVMNNARRVKIAS
                     QLLSETPNETSISKLSERYFISGASIVNDLRVIESWLAPLGLSLIRSPSGTHIEGSEG
                     QVRQAMALLINGIINHNEPQGVVYSRLDPGSYKALVHYFGEEEVLFVQSLLLDMENEL
                     SWSLGEPYYVNIFTHILIMMYRNTHGNALSREEDQTRQYDENIFNVASQMIHKIEQRI
                     AHTLPDDEVWFIYQYIISSGVAIDGQKDVSIISHMQASNEARLITWRLITVFSDIVDC
                     DFSEDSALYDGLMVHIKPLINRLNYRIHIRNPLLEDIKAELADVWRLTQYVVNQVFKT
                     WGENAVSEDEVGYLTVHFQAAMERQIARKRVLLVCSTGIGTSHLLKSRILRAFPEWTI
                     VDVISAANLSQVLPDNIELIISTINLPTVTMPVAYVTAFFNDADIKRVTEMVITEKLH
                     HATSRVVEI"
     gene            complement(3237..5555)
                     /gene="yicI"
                     /locus_tag="PN977_004164"
     CDS             complement(3237..5555)
                     /gene="yicI"
                     /locus_tag="PN977_004164"
                     /EC_number="3.2.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-xylosidase"
                     /protein_id="HDI6044142.1"
                     /translation="MKISDGNWLIQPGLNLIHPLQVFEVEQQGNEMVVYAAPRDVRER
                     TWQLDTPLFTLRFFSPQEGIVGVRIEHFQGALNNGPHYPLNILQDVKVTIENTERYAE
                     FKSGNLSARVSKGEFWSLDFLRNGERITGSQVKNNGYVQDTNNQRNYMFERLDLGVGE
                     TVYGLGERFTALVRNGQTVETWNRDGGTSTEQAYKNIPFYMTNRGYGVLVNHPQCVSF
                     EVGSEKVSKVQFSVESEYLEYFVIDGPTPKAVLDRYTRFTGRPALPPAWSFGLWLTTS
                     FTTNYDEATVNSFIDGMAERNLPLHVFHFDCFWMKAFQWCDFEWDPLTFPDPEGMIRR
                     LKAKGLKICVWINPYIGQKSPVFKELQEKGYLLKRPDGSLWQWDKWQPGLAIYDFTNP
                     DACKWYADKLKGLVAMGVDCFKTDFGERIPTDVQWFDGSDPQKMHNHYAYIYNELVWN
                     VLKDTVGEEEAVLFARSASVGAQKFPVHWGGDCYANYESMAESLRGGLSIGLSGFGFW
                     SHDIGGFENTAPAHVYKRWCAFGLLSSHSRLHGSKSYRVPWAYDDESCDVVRFFTQLK
                     CRMMPYLYREAARANARGTPMMRAMMMEFPDDPACDYLDRQYMLGDNVMVAPVFTESG
                     DVQFYLPEGRWTHLWHNDELDGSRWHKQQHGFLSLPVYVRDNTLLALGNNEQRPDYEW
                     HEGTAFHLFNLQDGHEAICEVPAADGSVLFTLKAARTGNTITVNGTGEAKNWTLCLRN
                     VVKVNGLQGGSQAESELGLVVTPQGNALTITL"
     gene            complement(5565..6947)
                     /locus_tag="PN977_004165"
     CDS             complement(5565..6947)
                     /locus_tag="PN977_004165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418114.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside-pentoside-hexuronide family
                     transporter"
                     /protein_id="HDI6044143.1"
                     /translation="MKSEVLSVKEKIGYGMGDAASHIIFDNVMLYMMFFYTDIFGIPA
                     GFVGTMFLVARALDAISDPCMGLLADRTRSRWGKFRPWVLFGALPFGIVCVLAYSTPD
                     LSMNGKMIYAAITYTLLTLLYTIVNIPYCALGGVITNDPTQRISLQSWRFVLATAGGM
                     LSTVLMMPLVNLIGGDNKPLGFQGGIAVLSVVAFMMLAFCFFTTKERVEAPPTTTSMR
                     EDLRDIWQNDQWRIVGLLTIFNILAVCVRGGAMMYYVTWILGTPEVFVAFLTTYCVGN
                     LIGSALAKPLTDWKCKVTIFWWTNALLAVISLAMFFVPMQASITMFVFIFVIGVLHQL
                     VTPIQWVMMSDTVDYGEWCNGKRLTGISFAGTLFVLKLGLAFGGALIGWMLAYGGYDA
                     AEKAQNSATISIIIALFTIVPAICYLLSAIIAKRYYSLTTHNLKTVMEQLAQGKRRCQ
                     QQFTSQEVQN"
     gene            7240..7334
                     /locus_tag="PN977_004166"
     tRNA            7240..7334
                     /locus_tag="PN977_004166"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7274..7276,aa:Sec,seq:tca)
     gene            7497..8660
                     /locus_tag="PN977_004167"
     CDS             7497..8660
                     /locus_tag="PN977_004167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001218976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDI6044144.1"
                     /translation="MALTDIQIKRAKPQDKPYTLNDGQGLSLLINPDGSKGWRFRFRF
                     AGKARLMSFGSYDLVSLAEAREKRDIARKQVANGIDPVEERKALRLAQKLSTENSFEA
                     ICREWHTNKADRWTVAYREEIIKTFEQDVFPFIGKRPISEIKPLELLEVLRRIEKRGA
                     LEKTRKVRQRCGEVYRYAIITGRAEYNPAPDLAIALAVPKQKHHPFLSAEELPHFIRD
                     LEAYTGSIITKNATKIVMLTGVRTQEMRFATWEEVDLEKGIWEIPAERMKMRRPHIVP
                     LSTQVVDLFKQLKPITGHYPYIFIGRNNRSKPISKESVSQVIELIGYKGRATGHGFRH
                     TMSTILHEQGFDSAWIEIQLAHVDKNRIRGTYNHAQYLEHRKKMMQWYSDKLY"
     gene            8673..11392
                     /locus_tag="PN977_004168"
                     /pseudo
     CDS             8673..11392
                     /locus_tag="PN977_004168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000365012.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SIR2 family protein"
     gene            complement(11594..>11745)
                     /locus_tag="PN977_004169"
     CDS             complement(11594..>11745)
                     /locus_tag="PN977_004169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Polarity suppression protein"
                     /protein_id="HDI6044145.1"
                     /translation="YSAQDSDILTTIGFRPDAASVDDNREKFTPAQNMIFSRKSAQLA
                     SRQSV"
BASE COUNT         3591 a         2663 c         2352 g         3139 t
ORIGIN      
        1 ggaagcgcga tccgccctgc atcgactcga gcaaatgaat cagtgcttct tgtaaccagg
       61 cgatgggttt accaatcagc gtcatcatta acagcccacc gataatggtg cttaataccg
      121 gcaacaccat gattggcatg agtccttgca tcgactgcgg caggcggata tagcgtttaa
      181 gtagcaaaat ggtgtagccc accaggaaac cacccaacat accgccaata aatccggtat
      241 ggatttgccc acagagaaag ccgacaatca ggcccggggc aaaaccggga cgatcagcaa
      301 tagaataggc aattgccgcg ctaatcagcg gcacaatcag ccccattccc cagccgccaa
      361 tctgattaag catccacggg attgttcctg ttttttcacc aacatctgta ccgccaagaa
      421 cttgccccaa cgcaatacat atcccggcgg ccacaataag gggtatcatc cacgagatgc
      481 cggttaataa atgtttttta atttcctgtg aaaccggaat tttattttct ttcatattaa
      541 gatccctcca gtataaggat catttgtttc ccagtgattt ttcgaccttt tcaagaaatt
      601 gaacgggcga tttaatgacg atctctgtct ttactcgcac aatacgttta ttggtgaagc
      661 gttcttctcc ttttattttc acatctacag caagaatcac gacgtcagca gcagagatat
      721 ctgccgcctg taattcattt tctgtaccaa tggttccctg ggtttcaact ttaatggtat
      781 ggcccagagc ttttgctcct ttgattaact tttcacgagc aatataggta tgagcaatac
      841 ccgccgtaca ggcggcaaca cagacaatat tcatgacata ctccctgtta tttagcagac
      901 atcggattgt gcatactgac taaagagatc aatgattttt tgcggactcg tctcaactaa
      961 caattgttct atgatgtcat catccgccag cgcgcccgcg acctgggaaa gtaaacggat
     1021 atgggtggta ttttgatctt caagacgtac cgcaaataaa ataatacagc gcacgctact
     1081 gccatccagc gtttcccagg gaatatcctg gcgggtacgg ccaattgcca gcgtagtatt
     1141 aattaccgca gaagatttac catgcgggat cgcaatatga ttttcaaaac cagtcgagcc
     1201 ttccgcttcg cgctgccaga catctttaat aaaggtttcc cgacaatcga tcgcaccatc
     1261 ttttttgaga agattagtta attcttcaat cgcctccgct ttattcgtcg cttgcatatc
     1321 taaaataacg cgattcgtat tcaggatttt tgttatgtcc attcgtcata cctcataaaa
     1381 ttctgacatt aaatttcaac gacccgagac gtcgcatgat gtaatttttc cgtaatcacc
     1441 atttcagtga cccgcttaat atcggcatca ttaaaaaaag cggtaacata agcgaccggc
     1501 atagtgactg taggcaaatt aattgtcgaa ataatcagtt cgatattgtc aggcaaaacc
     1561 tgtgataaat tcgctgctga aataacatca acaatcgtcc attcaggaaa tgctcgcaga
     1621 atacggcttt tcagtagatg cgaagttccg attccggttg aacagaccag taatacacgt
     1681 ttacgggcaa tttgccgctc catcgcagcc tgaaaatgaa cggtcaggta acccacttca
     1741 tcctcgctca ctgcattctc accccaggtt ttaaatacct gattcaccac atattgcgtc
     1801 aaccgccaga catccgctag ttctgcttta atatcttcca acaatggatt acggatatga
     1861 atacgataat ttagtcggtt aatcagcggt ttaatatgca ccattaagcc atcatataat
     1921 gcgctgtctt cactaaaatc gcagtccacg atgtcactga ataccgtaat taaacgccag
     1981 gtaatcagac gcgcttcatt gctggcctgc atatgtgaaa taatgctcac atctttttgt
     2041 ccatcaatcg ccacacctga tgaaatgata tattgataaa taaaccagac ttcatcatcg
     2101 ggcaatgtat gtgcaattcg ttgttctatc ttatgaatca tctgactggc aacattaaag
     2161 atattttcat catattgcct ggtttgatct tcttctcttg ataacgcatt cccgtgcgtg
     2221 ttgcgataca tcataataag gatgtgagta aaaatgttaa cgtaataagg ttctcccaaa
     2281 gaccaactta attcattttc catatccagt aacaatgact ggacaaataa cacttcttcc
     2341 tctccaaaat aatggactaa tgctttatag cttccgggat ccagacgtga atacacgaca
     2401 ccttgcggct cattatggtt aataatgccg ttaatcagta atgccattgc ctgtcggact
     2461 tgcccttcac taccttcaat atgcgtacca cttgggctgc ggatcaatga taaccccaac
     2521 ggcgcaagcc aggactcaat tactctcagg tcattaacaa tagaggctcc gctgataaag
     2581 tagcgttctg acaatttact gatcgacgtt tcattcggtg tttcacttaa taactgcgac
     2641 gctattttaa cgcgcctggc attattcatt acaccatcaa tctcttcaca ttctgctccc
     2701 aacaaatgat caagatgcag caaatcagca atattctccg cgcttaacat cacacccgcg
     2761 ccagcacgct tctcaacatt tattctccat tgccccagcc attgctcaag taaacgtaaa
     2821 tcacgttgaa tggttttttc cgaaacattt aaatagccgg caattgtaac aatagtcgta
     2881 tattcccttc gtggtagaag gaatcgtaat aatctgtttt gtcgcgaggt aatcatttgc
     2941 attttttacc cttatcacag attaagaagc cccatatgga agagcttcag ttgccatctc
     3001 tgagggtaaa tataaagcag aataatttca caacaatact aaaatatgtc cgtaatccgg
     3061 acagtgataa aacaaagcaa ggcggttaag ggatattttt cagcacagat cacattttcg
     3121 tgatgaataa tcgtgatcat gctcgcaaat acgatggaag aaatatttat taccagatgt
     3181 gagattattt accggatacg acacaaaacc attcgtatcc ggcattcttc aatagattac
     3241 aacgtaattg tcagcgcatt cccttgaggc gtcaccacca gccccagctc actttcagcc
     3301 tgcgaaccgc cttgcagacc atttactttc acaacattgc gcaagcacag cgtccagttc
     3361 ttcgcctcgc ccgtaccatt cacagtaatt gtgttgccag tacgcgccgc tttcagggtg
     3421 aaaagaacgg aaccatcggc agcgggcact tcacagatgg cttcatgccc gtcttgcaga
     3481 ttaaagaggt ggaatgccgt gccttcgtgc cactcgtaat cgggacgttg ctcgttgttg
     3541 cccagcgcca gtagggtgtt atcacgcaca taaacgggca gactcaggaa gccgtgctgc
     3601 tgtttatgcc agcgactacc atcgagttca tcgttgtgcc acaggtgtgt ccagcgacct
     3661 tccggcaagt agaactgcac atcgcccgat tcagtgaaca ccggagcaac catcacgttg
     3721 tcgcctaaca tgtattgacg gtcaaggtaa tcacaagccg gatcgtccgg gaactccatc
     3781 atcatggccc gcatcatcgg cgtaccccgc gcgttcgcac gagcagcctc acgatacaga
     3841 tacggcatca tgcggcattt cagttgcgtg aagaagcgca ccacatcaca ggactcatca
     3901 tcataggccc acggcacacg ataagatttg ctgccgtgta aacggctatg gctggagagc
     3961 aaaccaaacg cgcaccagcg tttgtaaacg tgcgccggag cggtattttc aaagccgccg
     4021 atatcgtggc tccagaagcc aaaacctgaa aggccaatag acaaaccacc gcgcaggctt
     4081 tccgccattg attcgtagtt agcgtaacag tcgccacccc agtgtaccgg aaatttctgc
     4141 gcaccaacgg aggccgagcg ggcaaacaag acggcttctt cctcaccaac ggtgtccttg
     4201 agcacgttcc acaccagttc gttgtagatg tacgcataat ggttatgcat tttctgcgga
     4261 tcggaaccgt caaaccattg aacatcggtt ggaatacgtt cgccaaagtc ggtcttaaag
     4321 caatcaacgc ccatcgcgac cagacctttc agtttgtcgg cgtaccattt gcaggcatcc
     4381 ggattggtaa agtcataaat cgccagacct ggctgccatt tatcccactg ccacagcgaa
     4441 ccgtccgggc gtttgagtaa ataacctttc tcttgtaact ctttaaagac aggggatttt
     4501 tgcccgatat aagggttaat ccagacgcag atttttagcc ctttcgcttt cagacggcgg
     4561 atcatccctt ccgggtccgg gaaagtcagc gggtcccact caaaatcgca ccactggaag
     4621 gctttcatcc agaaacagtc aaagtggaaa acatgcagcg gcagattgcg ttccgccata
     4681 ccatcgataa agctgtttac cgtcgcttcg tcgtagttgg tggtaaatga agtggttagc
     4741 cacagaccga aggaccacgc gggcggcagc gccggacgac cagtaaaacg ggtataacga
     4801 tcaagtaccg ctttcggcgt cgggccgtcg ataacaaagt attcgagata ttcactctca
     4861 acgctgaact gcactttgga gactttctcc gatcccactt caaaagagac gcattgagga
     4921 tgattgacca gtaccccata accacggtta gtcatataga acggaatatt tttatacgcc
     4981 tgttcagtac ttgtgccgcc gtcccggttc caggtctcta ccgtttggcc attgcgcacc
     5041 agggcagtaa agcgctctcc cagaccgtaa actgtttcgc caacgccaag atccagccgc
     5101 tcaaacatat aatttcgttg attattcgtg tcctgcacgt agccattatt tttcacctga
     5161 ctaccggtaa tacgttcgcc gttgcgcaga aaatccagtg accagaactc acctttgctg
     5221 acacgcgcac ttaagttgcc acttttaaac tcagcgtaac gttctgtgtt ttcgattgtg
     5281 accttcacgt cctgcaaaat attgagcggg taatgaggac cgttattcag cgccccctga
     5341 aaatgctcaa tccgtacacc gacaatacct tcctgtgggg agaaaaagcg cagcgtaaat
     5401 aaaggcgtat caagctgcca ggtacgttca cgcacatcac ggggggcagc atagaccacc
     5461 atttcattac cctgctgttc aacctcgaac acctgaagcg ggtgaatcaa attgaggcca
     5521 ggttgaatca accagtttcc gtcgctaatt ttcattgccg ttccttagtt ctgcacttct
     5581 tgagaggtga attgttgctg gcaacggcgt ttgccctggg ccagctgttc cataacggtt
     5641 ttcagattgt gcgtcgtgag tgagtagtag cgtttagcga taatcgcgct cagcaaataa
     5701 cagatcgccg gaacaatcgt gaatagcgcg ataatgatgc taatcgtggc gctgttctgc
     5761 gctttttctg ccgcatcata tccgccataa gccagcatcc agccgataag agcgccgccg
     5821 aaggccaacc ccagtttgag cacaaacagc gtgccagcaa aactgatccc ggtcaggcgt
     5881 ttaccattac accactcgcc gtagtcgacg gtatcggaca tcattaccca ctggataggt
     5941 gtcaccagtt gatgcaacac accaatcacg aagatgaaga caaacatggt gatgctggcc
     6001 tgcataggaa caaagaacat cgcgaggcta atcactgcca gcagggcgtt cgtccaccag
     6061 aagatagtga ctttacattt ccagtcggtc agcggtttcg ccagtgcgga accaatcagg
     6121 ttacccacgc aataagtggt aagaaaagcg acaaacactt ccggcgtacc caaaatccat
     6181 gtgacgtaat acatcatcgc cccgccgcgt acgcataccg ccagaatatt gaaaatggtt
     6241 agtaaaccga caatacgcca ctggtcgttt tgccagatat cacgtaaatc ttccctcata
     6301 gacgtcgttg taggtggtgc ttcaacgcgt tctttagtgg tgaagaaaca aaatgccagc
     6361 atcatgaatg ccaccacgga aaggaccgcg ataccgccct ggaaaccgag tggtttatta
     6421 tcaccaccaa ttaaattaac cagcggcatc atcagaacag tagagagcat gcctcccgca
     6481 gtcgccagca caaaacgcca ggattgcagc gagatacgct gagtcgggtc attggtgatt
     6541 acaccaccca atgcgcagta agggatatta acgatggtat aaagtaaggt aagtagggtg
     6601 taagtaattg ctgcataaat catttttccg ttcatactga gatctggcgt gctataggcc
     6661 agtacacaga cgatcccaaa tggcagtgcg ccaaacagta cccacggacg aaatttaccc
     6721 cagcgagagc gcgttcgatc ggccaacaac cccatgcaag gatcggaaat cgcatccagt
     6781 gcacgagcga ccaaaaacat ggttccgaca aatccggcag gaatgccaaa aatatcggta
     6841 taaaagaaca tcatatataa cattacgtta tcgaaaataa tgtggctggc ggcgtctccc
     6901 atgccataac caattttctc tttaacggac aacacttcac tcttcatctg cttttccttg
     6961 atgctatagg ggtgctgaga ccgataacgc attttgtaaa ccatccctgc gagagcgaat
     7021 attccgatat ctggttatta atttatgatt cttgttttat gtgatcgtgg tagcgtaaat
     7081 tacgctcata tatcattgta aagtatgggt tttatatgaa ctataatgct ttcgtgataa
     7141 tacgctgcat gtattaggcg gaaaaaactg atctggggga tgtagaaact caaggaagta
     7201 gctataatgc gccccgcctc catgtagcaa tcgaggcgcg gaagatcgtc gtctccggtg
     7261 aggcggctgg acttcaaatc cagttggggc cgccagcggt cccgggcagg ttcgactcct
     7321 gtgatcttcc gccaaacttc atccagccag ttccgagatg atccataaaa ccctttatat
     7381 tcaatatgat gaataatcat cttatccgta acagtccgag atcgtctccc taaatccgcg
     7441 aaaaatatgt atagtaatgt gtatagattt tttataagta gttttcagtt atacacatgg
     7501 ctctcaccga tatacagatc aaacgtgcaa aaccacaaga caagccatac acattgaacg
     7561 atggacaagg tctgtcattg cttatcaatc ccgatggctc gaaaggctgg cgtttccgtt
     7621 tccggtttgc agggaaagcg cggttaatgt catttggcag ctacgattta gtaagcctcg
     7681 cagaagcacg tgagaagcgt gatatcgccc gtaagcaggt tgctaatggc attgacccgg
     7741 tagaggaacg caaagcttta agactcgccc aaaagctatc aacagaaaat tctttcgaag
     7801 caatatgtcg agaatggcat accaacaaag ctgaccgctg gactgtggcc tatcgagaag
     7861 aaatcattaa gacattcgag caagatgtct tcccgttcat tggtaaacgt cctatcagtg
     7921 aaatcaaacc attagaactg cttgaagtat tacgacgaat cgaaaaacgt ggagcactag
     7981 agaaaacacg caaggtgcgt caaagatgcg gtgaggttta tcgctatgca atcataactg
     8041 gccgtgctga gtacaatcct gcacctgatt tagctatcgc tctggccgtt cccaagcaaa
     8101 aacaccatcc atttttatcc gctgaagagt tgcctcattt tattcgagat cttgaagcgt
     8161 ataccggtag catcatcacc aaaaatgcta cgaagatagt catgctgact ggtgtaagaa
     8221 cgcaggagat gcgctttgct acgtgggaag aagtagacct cgaaaaaggt atatgggaga
     8281 taccagcgga acgtatgaaa atgcgtagac ctcacattgt tcctttatct actcaggtag
     8341 ttgacctttt caaacagctc aaacctatta ccggccatta cccttacatc tttattggca
     8401 ggaacaaccg cagcaagcca atctcaaaag aaagtgtttc acaagtgatt gagttaattg
     8461 gctacaaagg ccgtgctaca ggtcacggtt ttcggcatac catgtcgaca atattgcacg
     8521 aacaagggtt tgatagcgca tggattgaaa tacaattggc acatgttgat aaaaacagaa
     8581 tccgagggac ttacaatcat gctcaatatc ttgaacatag aaaaaaaatg atgcaatggt
     8641 attcagataa attatattga gaggtatata caatggattt gctaaacaaa catatagcta
     8701 gtattttaag agcaaaagat gaggagtctt tagctatttt tgtaggggct ggtgtttcaa
     8761 aatcctctga aacaaaaact atcaaaatgc cttcatgggg ggacttaatt gacactctca
     8821 tttcagatct caatatcaaa gatgaatcag attatttaaa aatagcacag ctttattacc
     8881 tcacatttgg tgagcatctt tactacaaaa gaatcaaaga tttctttcct gaaaatgttc
     8941 cccattcaaa gattcacgat ctaatattta aactgaatcc tcactctgtt atcacaacga
     9001 attgggacac tttgcttgaa gcagctataa atgccaaatc ttacttttat aatataataa
     9061 gcagtgacaa agatttgatg aaatcttacc ttggcaaaaa attgataaaa atgcatggtg
     9121 attttaaaaa tcacaacatc gtctttaaag aagatgatta cttaaattat agcttcaatt
     9181 ttcccttaat tgaaaattat gttaaaagtg ttatttccac ccatactgtg ctatttttgg
     9241 ggtattcata taatgatata gatctaaagc aaataattaa atggactcag aatcattcat
     9301 ctgttagacc ccctatgtat ttagtagtat tcgaagatat tcctgctcaa agaaaatatc
     9361 ttgaaagtca tggtataacc actattattt taaccgatga aacaatcaaa cctttcaata
     9421 acaattcata ttcaaacaag ttatatactt tcctgcatag cctaaatagc ctagaactat
     9481 gtgcagactt agatgatatc gaaataatta atgctattta ttcaagagtg agatctcttc
     9541 aatcgttaaa tgcaatactt gctgaacaag ttaccagatg tttcactaat tgcggtttaa
     9601 tgtatattga tgacaatgga ccaaaagctt tattgcaatt ttatgacacc gaagtaacct
     9661 ctaatgataa taatatagag ctaagaggat tttataagaa gtttttgagt atactcagta
     9721 atgataaaaa agttaaagac tataagccgc atctccaaaa attatttttt atattaaaaa
     9781 aagcaagtat atatggtatt gttttaaacg ataaacatga tgaagtgcta ctgattactg
     9841 aagtgctacc aaatgattca ttaattaaaa tagataagga aattaatttc aactataacg
     9901 aaatagttac ctatacacgc ccccataagg ttgcaaatag tatagacaag actaagaaat
     9961 ataattgctt ccagttaaat aaatacgatg aggcttacag tattatagag gaggaattat
    10021 cagaagagat cagacaaaag gactatgcaa atattctcat atcgcttttc aatcaaaata
    10081 ttattttgaa tagattgaag tatgattttt caattaatag agaaagatac tcaactcttg
    10141 aggagaataa aattcatgaa ttatatgata acttaccgag aaatataaaa aaaccgtctc
    10201 cgtaatctat gatttagtaa cctttaatta tttattaaat cttcactaca ccgttagctc
    10261 attattaaca aagtatagcg atataagaaa aagaaataca aagttattag ttgacgggga
    10321 tcttcataaa acggaatttc ttttcgaaaa ccttataatt tttgttgtaa aaaatggatg
    10381 tttaattgat gtttataaag aattcaagga tgtgattcgc aaattcattg aaataaaaat
    10441 aatcaaggat tctgataaag acgagatttc tctaacaaga ttagaattat attcatgcat
    10501 taaatacatt gataataaaa ccctatctct tatactaagg aaagaagata aaaaactatt
    10561 atcgctgtct gttcaaccca aagaattaga ttggttaata aatactgtac tgcaaaacct
    10621 agcgaagtca tatagcaagt tcgctacgtt tctcaaccct atagaaggaa agttaatcaa
    10681 cgcattaaaa ctactatctt taatgaaaat aaccacagag caagacgcgg tagtattaaa
    10741 aacattaaac gacactttaa agtcctcata ccacaaccta gcattctatg atgctatttc
    10801 cgaatatgtc gttataaggt acaacaccca cagtgaaact ttatccactg acagcataaa
    10861 aactttaata tacacgattc tcgataagtt aataagtaga aaccttggta ggtatgaggt
    10921 gatagctata gtcaatagag gccttgctaa tattttttca gtagccaaaa aactgggcgt
    10981 taatatagaa gacgacagca aggtcgataa attacttcat gaaattagtt cttatccgaa
    11041 tacagataaa gcaagagctg ctgaaacaat actatatgat ctgtacagaa tatcaacaga
    11101 aaagaataga gataaaataa aatcatttat caaaaatatt tccacaactg atttcaatga
    11161 agaaagaaag attaaatttg aattattttt attagcatca gagatttcag acagctatga
    11221 caatcttcct gagaaagtat ccaagcttgt tgaaaactat aaaggattta gatttaactc
    11281 agaagcagag acaatcagag gtttattacg ctatatagtc aacacacgga aattaagtga
    11341 tttctcacaa gcacttttga agattgaaga gataataaat aattacaaat aatttcgttc
    11401 ctagcgcgca gtgctttccc cgcctcgccc gcccgctttg cggggcggtt ttaatgcagt
    11461 tgcactgaca cgctcagacc gcgccgggaa tggcgcggtc tgcagaaaat gaggcaggaa
    11521 aacgcatgca aagccatgca ccttatcgat gcatggcttt tttcagtaaa aacgggcgga
    11581 ttttcgggga attttacaca gactgacgtg atgccagttg cgcactttta cgcgaaaaaa
    11641 tcatgttctg cgcaggggtg aatttttcac ggttgtcatc caccgaagcc gcgtcaggcc
    11701 tgaatccgat ggtcgttaaa atgtcgctat cctgcgcgga ataat
//
LOCUS       DAMZFT010000145        11588 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.145, whole genome shotgun sequence.
ACCESSION   DAMZFT010000145 DAMZFT010000000
VERSION     DAMZFT010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11588)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11588)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11588
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.145"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..712
                     /gene="menB"
                     /locus_tag="PN977_004170"
     CDS             <1..712
                     /gene="menB"
                     /locus_tag="PN977_004170"
                     /EC_number="4.1.3.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001641973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA synthase"
                     /protein_id="HDI6044146.1"
                     /translation="PLTVKEMIQALADARYDDNIGVIILTGAGDKAFCSGGDQKVRGD
                     YGGYKDDSGVHHLNVLDFQRQIRTCPKPVVAMVAGYSIGGGHVLHMMCDLTIAADNAI
                     FGQTGPKVGSFDGGWGASYMARIVGQKKAREIWFLCRQYDAKQALDMGLVNTVVPLAD
                     LEKETVRWCREMLQNSPMALRCLKAALNADCDGQAGLQELAGNATMLFYMTEEGQEGR
                     NAFNQKRQPDFSKFKRNP"
     gene            712..1674
                     /gene="menC"
                     /locus_tag="PN977_004171"
     CDS             712..1674
                     /gene="menC"
                     /locus_tag="PN977_004171"
                     /EC_number="4.2.1.113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HDI6044147.1"
                     /translation="MRSAQVYRWQIPMDAGVVLRDRRLKTREGLYVCLREGEREGWGE
                     ISPLPGFSQETWEEAQSVLLAWVNNWLAGDCELPQMPSVAFGVSCALAELADTLPQAA
                     NYRAAPLCNGDPDDLILKLADMPGEKVAKVKVGLYEAVRDGMVVNLLLEAIPDLHLRL
                     DANRAWTPLKGQQFAKYVNPDYRHRIAFLEEPCKTRDDSRAFARETGIAIAWDESLRE
                     PDFAFVAEKGVRAVVIKPTLTGSLDKVREQVQAAHALGLTAVISSSIESSLGLTQLAR
                     IAAWLTPDTIPGLDTLDLMQAQQVRRWPGSPLPLVEVDALERLL"
     gene            1671..3026
                     /gene="menE"
                     /locus_tag="PN977_004172"
     CDS             1671..3026
                     /gene="menE"
                     /locus_tag="PN977_004172"
                     /EC_number="6.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000577608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate--CoA ligase"
                     /protein_id="HDI6044148.1"
                     /translation="MIFSDWPWRHWRQVRGEAIALRLNDEQLNWRELCARVDELASGF
                     VAQGVVEGSGVMLRAWNTPQTLLAWLALLQCGARVLPVNPQLPQPLLEELLPNLTLQF
                     ALVPDGENTFPALTSLHIQLVEGAHAAAWQPTRLCSMTLTSGSTGLPKAAVHTYQAHL
                     ASAQGVLSLIPFGEHDDWLLSLPLFHVSGQGIMWRWLYAGARMTVRDKQPLEQMLAGC
                     THASLVPTQLWRLLVNRSSVSLKAVLLGGAAIPVELTEQAREQGIRCFCGYGLTEFAS
                     TVCAKEADGLADVGSPLPGREVKIVNDEVWLRAASMAEGYWRNGQRVPLVNDEGWYAT
                     RDRGEMHNGKLTIVGRLDNLFFSGGEGIQPEEVERVIAAHPAVLQVFIVPVADKEFGH
                     RPVAVVEYDQQTVDLDEWVKDKLARFQQPVRWLTLPPELKNGGIKISRQALKEWVQRQ
                     D"
     gene            3136..3402
                     /gene="pmrD"
                     /locus_tag="PN977_004173"
     CDS             3136..3402
                     /gene="pmrD"
                     /locus_tag="PN977_004173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416762.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction protein PmrD"
                     /protein_id="HDI6044149.1"
                     /translation="MEWLVKKSCCNKQDNRHVLMLCDAGGAIKMIAEVKSDFAVKVGD
                     LLSPLQNALYCINREKLHTVKVLSASSYSPDEWERQCKVAGKTQ"
     gene            complement(3396..3782)
                     /gene="arnF"
                     /locus_tag="PN977_004174"
     CDS             complement(3396..3782)
                     /gene="arnF"
                     /locus_tag="PN977_004174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416761.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnF"
                     /protein_id="HDI6044150.1"
                     /translation="MGLMWGLFSVIIASAAQLSLGFAASHLPPMTHLWDFIAALLAFG
                     LDARILLLGLLGYLLSVFCWYKTLHKLALSKAYALLSMSYVLVWIASMVLPGWEGTFS
                     LKALLGVACIMSGLMLIFLPTTKQRY"
     gene            complement(3782..4117)
                     /gene="arnE"
                     /locus_tag="PN977_004175"
     CDS             complement(3782..4117)
                     /gene="arnE"
                     /locus_tag="PN977_004175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnE"
                     /protein_id="HDI6044151.1"
                     /translation="MIWLTLVFASLLSVAGQLCQKQATCFVAINKRRKHIVLWLGLAL
                     ACLGLAMVLWLLVLQNVPVGIAYPMLSLNFVWVTLAAVKLWHEPVSPRHWCGVAFIIG
                     GIVILGSTV"
     gene            complement(4114..5766)
                     /gene="arnT"
                     /locus_tag="PN977_004176"
     CDS             complement(4114..5766)
                     /gene="arnT"
                     /locus_tag="PN977_004176"
                     /EC_number="2.4.2.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A)
                     4-amino-4-deoxy-L-arabinosyltransferase"
                     /protein_id="HDI6044152.1"
                     /translation="MKSVRYLIGLFAFIACYYLLPISTRLLWQPDETRYAEISREMLA
                     SGDWIVPHLLGLRYFEKPIAGYWINSIGQWLFGANNFGVRAGVIFATLLTAALVTWFT
                     LRLWRDKRLALLAAVIYLSLFIVYAIGTYAVLDPFIAFWLVAGMCSFWLAMQAQTWKG
                     KSAGFLLLGITCGMGVMTKGFLALAVPVLSVLPWVATQKRWKDLFIYGWLAVISCVLT
                     VLPWGLAIAQREPDFWHYFFWVEHIQRFAMDDAQHRAPFWYYLPVIIAGSLPWLGLLP
                     GALYAGWKNRKHSATVYLLSWTIMPLLFFSVAKGKLPTYILSCFAPLAMLMAHYALLA
                     AKNNPLALRINGWINIAFGVTGIIATFVVSPWGPMNTPVWQTFESYKVFCAWSIFSLW
                     AFFGWYTLTNVEKTWSFAALCPLGLALLVGFSIPDRVMEGKHPQFFVEMTQESLQPSR
                     YILTDSVGVAAGLAWSLQRDDIIMYRQTGELKYGLNYPDAKGRFVSGDEFANWLNQHR
                     QEGIITLVLSVDRDEDINSLAIPPADAIDRQERLVLIQYRPK"
     gene            complement(5766..6656)
                     /locus_tag="PN977_004177"
     CDS             complement(5766..6656)
                     /locus_tag="PN977_004177"
                     /EC_number="3.5.1.n3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-deoxy-4-formamido-L-arabinose-
                     phosphoundecaprenol deformylase"
                     /protein_id="HDI6044153.1"
                     /translation="MTKVGLRIDVDTFRGTREGVPRLLEILSKHNIQASIFFSVGPDN
                     MGRHLWRLVKPQFLWKMLRSNAASLYGWDILLAGTAWPGKEIGHANADIIREAAKYHE
                     VGLHAWDHHAWQAHSGNWDRQTMVDDIARGLRTLEEIIGQPITCSAAAGWRADQQVIE
                     AKEGFHLRYNSDCRGVMPFRPLLDSGKPGTAQIPVTLPTWDEVIGRDVKAEDFNGWLL
                     NRILRDKGTPVYTIHAEVEGCAYQHNFVDLLKRAAQEGVTFCPLSELLSGMLPLGQVV
                     RGNIAGREGWLGCQQIAGSR"
     gene            complement(6653..8635)
                     /gene="arnA"
                     /locus_tag="PN977_004178"
     CDS             complement(6653..8635)
                     /gene="arnA"
                     /locus_tag="PN977_004178"
                     /EC_number="1.1.1.305"
                     /EC_number="2.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-4-amino-4-deoxy-L-arabinose
                     formyltransferase/UDP-glucuronic acid oxidase ArnA"
                     /protein_id="HDI6044154.1"
                     /translation="MKTVVFAYHDMGCLGIEALLAAGYEISAIFTHTDNPGEKAFYGS
                     VARLAAERGIPVYAPDDVNHPLWVERIAQLSPDVIFSFYYRHLIHDKILQLAPAGAFN
                     LHGSLLPKYRGRAPLNWVLVNGETETGVTLHRMVKRADAGAIVAQLRIAIAPDDIAIT
                     LHHKLCHAARQLLEQTLPAIKDGNILEIAQCENEATCFGRRTPEDSFLEWHKSAAVLH
                     NMVRAVADPWPGAFSYVGNQKFTVWSSRVHSHAPAAQPGSVISVAPLLIACGDGALEI
                     VTGQAGGGITMQGSQLAQTLGLVQGSRLNSQPACAARRRTRVLILGVNGFIGNHLTER
                     LLREDHYEVYGLDIGSDAISRFLNHPHFHFVEGDISIHSEWIEYHVKKCDVVLPLVAI
                     ATPIEYTRNPLRVFELDFEENLRIIRYCVKYRKRIIFPSTSEVYGMCSDKYFDEDHSN
                     LIVGPVNKPRWIYSVSKQLLDRVIWAYGEKEGLQFTLFRPFNWMGPRLDNLNAARIGS
                     SRAITQLILNLVEGSPIKLIDGGKQKRCFTDIRDGIEALYRIIENAGNRCDGEIINIG
                     NPENEASIEELGEMLLASFEKHPLRHYFPPFAGFRVVESSSYYGKGYQDVEHRKPSIR
                     NARRCLDWEPKIDMQETIDETLDFFLRTVDLTDKPS"
     gene            complement(8635..9603)
                     /gene="arnC"
                     /locus_tag="PN977_004179"
     CDS             complement(8635..9603)
                     /gene="arnC"
                     /locus_tag="PN977_004179"
                     /EC_number="2.4.2.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate
                     4-deoxy-4-formamido-L-arabinose transferase"
                     /protein_id="HDI6044155.1"
                     /translation="MFEIHPVKKVSVVIPVYNEQESLPELIRRTTKACESLGKEYEIL
                     LIDDGSSDNSAHMLVEASQAEGSHIVSILLNRNYGQHSAIMAGFSHVTGDLIITLDAD
                     LQNPPEEIPRLVAKADEGYDVVGTVRQNRQDSWFRKTASKMINRLIQRTTGKAMGDYG
                     CMLRAYRRHIVDAMLHCHERSTFIPILANIFARRAIEIPVHHAEREFGESKYSFMRLI
                     NLMYDLVTCLTTTPLRMLSLLGSIIAIGGFSIAVLLVILRLTFGPQWAAEGVFMLFAV
                     LFTFIGAQFIGMGLLGEYIGRIYTDVRARPRYFVQQVIRPSSKENE"
     gene            complement(9607..10746)
                     /gene="arnB"
                     /locus_tag="PN977_004180"
     CDS             complement(9607..10746)
                     /gene="arnB"
                     /locus_tag="PN977_004180"
                     /EC_number="2.6.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_024188371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-4-amino-4-deoxy-L-arabinose
                     aminotransferase"
                     /protein_id="HDI6044156.1"
                     /translation="MSEFLPFSRPAMGVEELAAVKEVLESGWITTGPKNQALEQAFCQ
                     LTGNQHAIAVSSATAGMHITLMALEIGKGDEVITPSLTWVSTLNMISLLGATPVMVDV
                     DRDTLMVTPEAIESAITPRTKAIIPVHYAGAPADIDAIRAIGERYGIAVIEDAAHAVG
                     TYYKGRHIGAKGTAIFSFHAIKNITCAEGGLIVTDNENLARQLRMLKFHGLGVDAYDR
                     QTWGRAPQAEVLTPGYKYNLTDINAAIALTQLAKLEHLNTRRREIAQQYQQALAALPF
                     QPLSLPAWPHVHAWHLFIIRVDEQRCGISRDALMEALKERGIGTGLHFRAAHTQKYYR
                     ERFPTLSLPNTEWNSERICSLPLFPDMTTADADRVITALQQLAGQ"
     gene            11054..>11588
                     /locus_tag="PN977_004181"
     CDS             11054..>11588
                     /locus_tag="PN977_004181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HDI6044157.1"
                     /translation="MLAFCRSSLKSKKYFIILLALAAIAGLGTHAAWSSNGLPRIDNK
                     TLARLAQQHPVVVLFRHAERCDRSTNQCLSDKTGITVKGTQDARELGNAFSADIPDFD
                     LYSSNTVRTIQSATWFSAGKKLTVDKRLLQCGNEIYSAIKDLQSKAPDKNIVIFTHNH
                     CLTYIAKDKRDATFKPDY"
BASE COUNT         2855 a         2951 c         3061 g         2721 t
ORIGIN      
        1 tcctctgacg gtaaaagaga tgatccaggc actggcagat gcgcgttatg acgacaacat
       61 cggcgtgatc attctgactg gcgcaggcga taaagcgttc tgctccggtg gtgaccagaa
      121 agtgcgtggt gattacggcg gctataaaga tgattccggc gtacatcacc tgaacgtgct
      181 ggacttccag cgtcagattc gtacctgtcc gaaaccagtt gtcgcgatgg tggctggcta
      241 ctccatcggc ggcggtcacg ttctgcacat gatgtgcgat ctgactatcg cggcagataa
      301 tgccatcttc ggtcagactg gcccgaaagt cggttccttc gacggcggct ggggcgcttc
      361 ctacatggct cgcatcgtcg gacagaaaaa agcgcgtgaa atctggttcc tgtgccgtca
      421 gtacgacgcg aaacaggcgc tggatatggg ccttgtaaac accgtggtac cgctggcgga
      481 tctggaaaaa gaaaccgtgc gctggtgccg tgaaatgctg caaaacagcc cgatggcgct
      541 gcgctgcctg aaagctgcac tgaacgccga ctgtgacggt caggcaggtc tgcaggagct
      601 ggcgggcaac gccaccatgc tgttctacat gacggaagaa ggtcaggaag gtcgcaacgc
      661 cttcaaccag aaacgtcagc ctgacttcag caaatttaaa cggaatccgt aatgcgtagc
      721 gcgcaggtat accgctggca gatccccatg gacgcggggg tggttctgcg cgacaggcgg
      781 ttaaaaaccc gcgaagggct gtacgtttgc ctgcgtgagg gcgagcgcga agggtggggg
      841 gagatctccc cactgccggg cttcagccag gaaacctggg aagaggcgca aagcgtgctg
      901 cttgcctggg taaataactg gctggcaggc gattgcgaat taccgcagat gccttccgtg
      961 gcctttggcg taagctgtgc attggcggag ctggcagata cgctcccgca ggcggccaat
     1021 taccgtgcgg caccactgtg taatggcgat cctgacgatc tgatcctcaa acttgccgat
     1081 atgcctggtg agaaagtggc gaaggtcaaa gtgggattgt acgaagccgt gcgcgacggc
     1141 atggtggtga atctgttgct ggaggcaatt ccggatctgc atttgcgtct tgacgcgaat
     1201 cgcgcctgga caccgctgaa aggccagcag tttgccaaat acgttaaccc ggattatcgc
     1261 caccgcattg cttttctcga agagccgtgc aaaacccgcg atgattcgcg tgcgtttgcc
     1321 cgtgaaaccg gcattgccat tgcctgggat gaaagcctgc gtgagccgga ttttgccttt
     1381 gtggcggaaa aaggtgtgcg cgcggtagtt atcaaaccca cgctcacggg cagcctggat
     1441 aaagtacgcg agcaggtgca ggcggcgcac gcgttggggc tgacggcggt gatcagttct
     1501 tctattgagt cgagcttagg cttaacgcaa ctggcgcgga ttgccgcctg gttaacgcca
     1561 gacaccattc cggggctgga cacgctggat ctgatgcagg cgcagcaggt tcgtcgctgg
     1621 ccgggtagcc cgctaccgtt agtggaagtt gatgcactgg agcggctgtt atgatcttct
     1681 ctgactggcc gtggcgtcac tggcggcaag tgcggggaga agccatcgcc ttacgtctta
     1741 atgacgagca acttaactgg cgcgagcttt gtgctcgcgt cgatgaatta gcctccggct
     1801 ttgtggcgca gggcgttgtt gagggcagcg gcgtgatgtt gcgggcgtgg aatacgccgc
     1861 aaacgctgct cgcctggctg gcgttactgc aatgcggggc gcgggtgttg cccgtgaacc
     1921 ctcagctgcc gcaaccgttg cttgaagaat tgctgcccaa tctgacgctg caatttgcgc
     1981 tggtgccgga tggggaaaac acgtttccgg cgttaacgtc gctgcacatt cagctggttg
     2041 aaggcgcaca tgccgctgcg tggcagccga cgcgtctgtg ctcaatgacg ttgacctccg
     2101 gctctaccgg tttgccgaaa gccgctgttc atacttatca ggcccatctt gccagtgcgc
     2161 aaggtgtgtt atcgctgata ccgtttggcg agcacgatga ctggttgctc tctttaccgc
     2221 tgtttcacgt ctccggtcag ggaattatgt ggcgctggtt atacgctggt gcgcggatga
     2281 cggtacgtga taaacagccc ctggagcaaa tgctggcagg ctgtactcac gcttcactgg
     2341 taccaacaca actctggcgt ttgctggtta accgtagttc cgtttccctg aaagcggttt
     2401 tacttggcgg cgcggctatc ccggtcgagt tgacggaaca ggcgcgcgag caggggattc
     2461 gttgcttttg cggctatggt ctgaccgagt ttgcctccac ggtgtgtgcg aaagaagccg
     2521 acggtctggc agacgttggt tcgccgctgc cgggtcggga agtgaaaatc gttaatgatg
     2581 aagtgtggct gcgggctgcc agtatggcag aaggttactg gcgtaacggg caacgggttc
     2641 cactggttaa tgacgaaggc tggtacgcca cgcgcgatcg cggtgagatg cataatggca
     2701 agctgaccat tgtcggacgt ttagacaatc tattcttcag tggcggtgag ggtattcagc
     2761 cggaagaagt cgagcgcgtt attgctgcac atcctgcggt tttgcaggtg tttatcgtcc
     2821 ccgttgccga caaggagttt ggtcatcggc cggtagcggt ggtggagtat gaccagcaaa
     2881 ccgttgatct tgacgaatgg gtgaaagaca agttggctcg ttttcaacaa ccggtgcgct
     2941 ggctgacttt gccgccggag ctgaaaaacg gcggtattaa aatttcacgt caggcgttaa
     3001 aagagtgggt gcaacgccag gattaagaaa tcattagttt cacttcgcaa ttttgccatt
     3061 ccattgccgt tgataatagg tttatctgtt aatctgtaaa ttgatgtgaa aactcttatc
     3121 aaacaggata atgcaatgga atggctggtc aaaaaatcgt gctgcaataa acaagataac
     3181 agacatgtac ttatgctctg cgatgctggc ggcgcgataa aaatgatcgc cgaagtgaag
     3241 agtgacttcg ccgtgaaagt gggggattta ctctcgcctt tgcagaatgc gctttattgt
     3301 attaatcgtg aaaagctgca tacggtgaaa gtcctctcgg caagcagtta tagccccgat
     3361 gagtgggaac ggcagtgtaa agtggcaggg aaaactcagt aacgttgttt tgtcgtgggc
     3421 aggaaaatca gcatcaaccc gctcataata caggctactc ccagtagtgc tttcagcgaa
     3481 aaagttcctt cccagccggg aagtaccatt gaagcaatcc ataccagcac ataactcata
     3541 ctgagcaagg cataggcttt gcttagtgca agtttatgta gtgttttata ccaacaaaaa
     3601 acggaaagca gataacccag cagcccgagt aacagtatcc gggcatccag gccaaaagct
     3661 aacagcgccg cgataaaatc ccataagtgc gtcatcggcg gcagatgact cgccgcaaaa
     3721 cccaggctta attgcgcagc ggaggcgata atgacgctga ataatcccca catcaggccc
     3781 attacacggt actcccgagg atcacaatgc caccaataat gaacgccacc ccacaccagt
     3841 gacgcggcga taccggttcg tgccacagtt ttactgcagc cagcgtcacc cagacaaaat
     3901 tcagacttaa catcgggtag gcaatgccta ccggtacgtt ctgcaagacc agcagccaga
     3961 gcaccatggc aagaccaaga caagccagcg ccagtcccag ccacagcacg atatgtttgc
     4021 gccgtttgtt tatcgccaca aagcaggttg cttgtttctg acacaactgc ccggcaacgc
     4081 taagcaagct ggcaaagact aatgttagcc agatcatttg ggacgatact gaatcagcac
     4141 cagacgctcc tgacgatcga tggcatctgc gggcggaatg gcgagactgt tgatatcttc
     4201 atcgcggtca accgaaagca cgagtgtaat aatcccctcc tgacgatgtt gattaagcca
     4261 gtttgcgaac tcatcaccgc tgacaaacct ccctttcgca tccggataat taaggccgta
     4321 tttcaactca cccgtctggc gatacatgat gatgtcatcg cgttgcaggc tccatgccag
     4381 acctgcggca acgcccacgc tgtcagtaag aatatagcgg cttggctgca gtgattcttg
     4441 tgtcatctcg acaaaaaatt gcggatgttt tccttccatg actctgtcag gaattgaaaa
     4501 tcctaccagc aacgccagcc ccagcgggca aagcgcggca aaagaccagg tcttttcgac
     4561 gtttgttaag gtgtaccagc cgaaaaatgc ccatagcgaa aaaatcgacc aggcacaaaa
     4621 gactttatag ctctcgaagg tttgccacac tggcgtgttc attggtcccc atggggagac
     4681 cacaaacgta gcaataatgc cagtgacacc aaaagcgatg ttaatccagc cattaatccg
     4741 cagcgccaga ggattatttt ttgctgccag caaagcgtaa tgcgccatca gcattgccag
     4801 aggtgcaaag caggaaagaa tataggtggg caatttgcct ttagcgacgg agaaaaacag
     4861 cagcggcatt atcgtccagc tcaacaaata gacggttgcg gaatgcttgc ggtttttcca
     4921 gcctgcgtac agtgctccgg ggagtaatcc cagccacggc aggctaccgg caatgatgac
     4981 cggcaagtag taccagaacg gagctctatg ctgagcatca tccattgcaa agcgttgaat
     5041 atgctcaacc cagaaaaaat agtgccagaa gtcaggctcc cgctgcgcta tcgccagtcc
     5101 ccaggggaga accgtcagta cgcaactgat aaccgccagc cagccgtaaa taaagagatc
     5161 tttccagcgt ttttgcgttg caacccatgg aagcacgctt aataccggca cggcaagggc
     5221 gagaaaacct ttggtcatca cccccatgcc gcaggtgatt cccagcagta aaaatcccgc
     5281 gcttttgcct ttccacgtct gtgcctgcat tgccagccag aagctgcaca ttcccgccac
     5341 cagccagaag gcaataaacg gatcgagcac ggcataagtg ccgatggcat agacaatgaa
     5401 caatgagagg taaatcactg cggcgagtaa tgccagacgt ttatcgcgcc ataagcgcag
     5461 agtaaaccag gtcacaagcg cggcagttaa cagggtcgca aagataacgc ctgcccgcac
     5521 accaaagtta ttcgcgccaa atagccattg cccaatgctg ttaatccagt atccagcaat
     5581 gggtttttcg aaataacgta gccctaacag atggggcaca atccagtcgc cggatgccag
     5641 catttctcga ctgatttccg cataacgcgt ttcatcgggt tgccatagca gacgcgtgct
     5701 gatcggtaac aggtaatagc aggcaataaa cgcgaagagg ccgataaggt aacgtaccga
     5761 tttcatcagc gactacccgc aatttgttgg caacccagcc agccttcacg tccggcaata
     5821 tttccgcgaa caacttgtcc gagcggcaac atccctgata acagttcgct taaagggcaa
     5881 aatgtcacgc cttcctgagc tgcgcgtttg aggagatcca caaaattatg ctgataagcg
     5941 cagccttcaa cttctgcatg aatggtataa accggcgtgc ctttatctcg caggatgcgg
     6001 ttgagtaacc aaccgttaaa atcttctgct ttcacatccc gaccaatcac ttcatcccag
     6061 gtgggtaagg tcaccgggat ttgcgcagtg ccgggttttc ctgagtcgag caagggacga
     6121 aacggcatca ccccacgaca atcgctgttg tagcgcaaat ggaacccttc ttttgcttcg
     6181 atcacctgtt ggtcggcacg ccagccagcg gcagcggagc aggttattgg ttggccgata
     6241 atctcttcca gggtacgaag gcctcgtgca atatcgtcga ccatcgtttg ccgatcccag
     6301 ttgccgctat gggcttgcca ggcgtggtga tcccaggcgt gcaggccgac ttcgtggtat
     6361 ttagccgctt cacgaatgat atcggcattg gcatgaccaa tctctttacc tggccaggcc
     6421 gtacctgcca gtaaaatatc ccagccataa agcgatgccg cgtttgagcg cagcatcttc
     6481 cacaaaaact gtggcttcac cagtcgccag agatggcggc ccatattgtc cgggccgacg
     6541 ctgaaaaaaa tgctggcctg aatattatgc ttactcaaga tttccagcag acgcggcacg
     6601 ccttcacggg tgccacgaaa ggtatcgaca tcaatgcgta agccgacttt ggtcatgatg
     6661 gtttatccgt aagatcaacg gtgcgcagga agaaatccag cgtttcgtcg atggtttcct
     6721 gcatatcaat tttcggttcc cagtccaggc agcggcgggc attgcggatg ctcggtttac
     6781 gatgctctac gtcctgatat cctttgccgt agtagctgct actttcgaca acacgaaagc
     6841 ccgcaaacgg tgggaagtaa tggcgcagcg gatgtttttc gaagctcgcc agcagcatct
     6901 cccccagttc ttcaatgctc gcttcgttct caggattgcc aatgttgata atttcgccat
     6961 cgcagcgatt tcccgcattt tcgataatgc ggtataacgc ctcgatacca tcccgaatat
     7021 cagtaaagca gcgtttttgt tttccgccat caatcagctt aatcggtgaa ccttctacca
     7081 gattgagaat gagttgcgta atagcgcggg agctgccaat tcgcgctgca ttaaggttat
     7141 ccagtcgtgg ccccatccag ttaaacgggc ggaagagggt gaactgtaaa ccttcttttt
     7201 cgccataggc ccagatcact cgatcaagta attgtttaga caccgaataa atccagcgtg
     7261 gtttattcac cgggccgacg attaaattag aatggtcctc atcgaagtat ttatcgctac
     7321 acatcccata aacttctgaa gtcgacggga agatgattcg cttacggtac ttcacgcagt
     7381 agcggataat gcgcagattc tcttcgaaat cgagttcaaa tacgcgcagc gggttgcggg
     7441 tatattcaat cggcgtggct atcgccacca aaggcaagac gacatcacat tttttgacgt
     7501 gatactcaat ccattcggaa tgaatactga tatcgccttc gacaaagtga aaatgcggat
     7561 gattcagaaa acggcttatc gcatcgctgc caatatccag accgtaaact tcataatgat
     7621 cttcgcgcag caggcgttct gtcagatggt tgccaataaa gccattcacc ccgaggatga
     7681 gtacccgggt acggcgtcgg gcggcacagg caggctggct attcaagcgt gaaccttgca
     7741 ccaggcccag cgtctgcgct aattgcgagc cctgcatagt aatgccgccg cccgcctgtc
     7801 cagtgacgat ttccagcgcg ccatcgccac aggcaatcag cagtggcgca acagaaatca
     7861 cgctccccgg ttgtgctgcg ggcgcatgag aatgtacgcg tgacgaccag acggtaaatt
     7921 tttgattacc aacatagctg aaggcacccg gccacggatc ggcgactgca cgcaccatgt
     7981 tatgcaatac tgctgccgat ttgtgccact cgaggaagct gtcttctggc gttctgcgac
     8041 caaaacaggt ggcttcgttt tcgcactggg cgatttccag aatattaccg tctttaatgg
     8101 cgggtaatgt ctgctccagt agctgtcgcg cggcatggca taacttatga tgcagcgtaa
     8161 tggcgatatc gtctggcgca atggcaatgc gcagttgggc tacaatggcc ccagcatcgg
     8221 cacgtttcac cattcggtgc aatgtcacgc cagtttccgt ttcaccgttc accagcaccc
     8281 agttcagcgg cgcgcgacca cgatattttg gtaacagtga accatgcaga ttaaatgcgc
     8341 ctgcaggagc gagctgcaaa atcttgtcgt gaataagatg gcgataataa aaagagaaaa
     8401 tcacatctgg tgatagttgg gcaatgcgtt ccacccacag cggatggtta acgtcatccg
     8461 gcgcataaac cggaatgcct ctttccgctg ccagacgagc caccgaacca taaaaggctt
     8521 tttcaccggg attatcagta tgggtaaaaa tggcgctaat ttcgtaaccg gcagccagca
     8581 gggcttcaat accgaggcat cccatatcgt ggtaggcaaa aacgacggtt ttcattattc
     8641 attttccttg ctggatggac ggataacttg ctgaacaaaa tagcgggggc gggcgcggac
     8701 atcggtgtag atcctgccga tatattcacc gagcaatccc atgccgataa actgagcgcc
     8761 aataaaagta aacagcacgg caaatagcat aaagacgcct tctgccgccc attggggtcc
     8821 gaaggtcagg cgtaaaatca ccaacagcac cgcaatgcta aaaccgccaa tcgcaataat
     8881 gctgccgagc agactcagca tacgtagcgg cgtagtggta aggcaggtca ccaggtcgta
     8941 catcaaatta atcaggcgca taaagctgta tttggattca ccaaactcac gctcggcatg
     9001 atgcactgga atttcaatgg cacggcgggc gaagatattc gccagaatcg ggataaaggt
     9061 gctgcgttca tggcagtgca acatcgcatc gacaatatga cggcgatagg cgcgcagcat
     9121 acaaccatag tcacccatcg ctttgccagt ggtgcgctga ataagccggt taatcatctt
     9181 cgaagcggtt ttacgaaacc agctgtcctg gcggttctgg cgtacagtcc ctaccacgtc
     9241 gtaaccttca tcggcttttg ccaccagacg ggggatttcc tctggcggat tctggagatc
     9301 ggcatcgagg gtaataatta agtcgccggt gacgtgactg aatcccgcca taatcgctga
     9361 atgttgcccg taattgcggt taagcaaaat agacacaata tggctgccct ccgcttgtga
     9421 ggcttcgacc agcatatgcg cggaattatc gctactgccg tcatcaatca gcaggatctc
     9481 atactctttc cccagtgatt cacaggcttt tgtggtgcgc ctgattaatt ccggtaagct
     9541 ttcctgctcg ttataaacgg gaataaccac cgagactttc ttaacagggt ggatttcaaa
     9601 catggcttat tgtcctgcga gttgctgaag ggctgtgatg acgcggtcgg catcggcggt
     9661 agtcatatcc gggaacagcg gcaacgaaca gatgcgttcg ctattccatt cggtattcgg
     9721 taacgacagc gtgggaaaac gctcgcgata atatttttgt gtgtgagcgg cgcggaaatg
     9781 taacccggta ccaatacctc tttcttttaa cgcttccatc aacgcatcgc gactgatacc
     9841 acaacgttgt tcatcgacac gaataataaa cagatgccag gcgtgaacgt gcggccaggc
     9901 gggaaggctt aatggctgaa aggggagagc tgccagtgct tgctgatatt gctgggcaat
     9961 ttcgcgccga cgggtgttga ggtgctctaa ttttgctaac tgtgtcaggg caatcgcggc
    10021 gttaatatcg gtcagattgt acttatagcc cggtgttaag acttcagctt gcggtgcacg
    10081 gccccaggtt tgtctgtcat aggcatcgac acccagaccg tgaaatttca gcatccgtag
    10141 ctggcgggca aggttttcat tatcagttac aatcaggcca ccttcagcac aggtaatatt
    10201 tttaatggca tgaaatgaaa aaatagcggt accttttgcg ccaatatgtc gccctttgta
    10261 atacgtaccg acggcatggg cagcatcttc gataactgcg atgccgtaac gttcgccaat
    10321 ggcgcgaatg gcgtcaatat ctgctggcgc accggcataa tgcacaggaa tgatggcttt
    10381 agtgcgtggc gtaatggctg actcgatcgc ttcaggcgtg accatcagcg tatcgcggtc
    10441 gacatccacc attaccggcg ttgcacccaa caaggagatc atattgaggg ttgaaaccca
    10501 ggtcagggaa ggcgtaatca cttcatcgcc cttgcctatt tccaacgcca ttagcgtgat
    10561 atgcattccg gcggtggctg aactgaccgc gatggcatgc tgatttcccg tcaactggca
    10621 aaaggcttgt tcaagcgcct gattcttcgg accggttgtg atccaaccgg attcgagaac
    10681 ctctttaact gcagcgagtt cctccacgcc cattgctggt cgcgaaaaag gcaaaaattc
    10741 tgacattgct tttccttccg ccattgaata cctgtccact tatatttgct ataaagagtg
    10801 ttgtgtatat tttgccattt ggagcaaaat tttaaggata gaatattaac ttaaccttaa
    10861 gaaactaata ttagacgtaa atattgaaat ttttatattt tttcttattt aggctttgca
    10921 tttggcaaaa ttttgaggca ttttgccggc atcgcaggat ttttaatatt acaccaactg
    10981 ctaattatcg ccagaaatgt cgctcaattt cacggtaatt gtctggttgc gcttgtctat
    11041 aggtggagtt tacgtgttag ctttttgccg ctcttcgttg aagtcaaaaa aatatttcat
    11101 cattttactg gcgctcgctg caattgccgg actgggtact catgccgcct ggagtagcaa
    11161 tggtttgcca cgtatcgaca ataaaacgct ggccagactg gcacagcagc acccggttgt
    11221 cgttttgttt cgccatgctg aacgttgcga tcgttcaacc aatcaatgct tgtcagataa
    11281 aacaggtatt acggtcaaag gtacccagga tgcccgtgaa ctgggcaacg cttttagtgc
    11341 tgatatccct gatttcgatc tttattccag taataccgtc cggaccattc agtcggcgac
    11401 ctggttttca gcgggtaaaa aattgacggt agataaacga cttcttcaat gcggtaatga
    11461 gatttatagt gcaattaagg acttacaaag caaagcacct gataaaaata tcgttatttt
    11521 tacccataat cattgcctga catatattgc taaagataag cgtgatgcga catttaaacc
    11581 tgattatc
//
LOCUS       DAMZFT010000146        11541 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.146, whole genome shotgun sequence.
ACCESSION   DAMZFT010000146 DAMZFT010000000
VERSION     DAMZFT010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11541)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11541)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11541
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.146"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..266
                     /locus_tag="PN977_004182"
     CDS             <1..266
                     /locus_tag="PN977_004182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
                     /protein_id="HDI6044158.1"
                     /translation="REGTLWCLVSSAAGANTCHGSSPLVFVPRWPEVEMSDLTPSLAF
                     FGLRNTAWAGHIRFKNSTGEWWLVVSPWGRLRLCQQGETEGCL"
     gene            257..820
                     /locus_tag="PN977_004183"
     CDS             257..820
                     /locus_tag="PN977_004183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
                     /protein_id="HDI6044159.1"
                     /translation="MPVKEQGFSLLEVLIAMAISSVLLLGAARFLPALQRESLTSTRK
                     LALEDEIWLRVFTVAKHLQRAGYCHGSCTGEGLEIVGQGDCVIVQWDANSNGIWDREP
                     VKESDQIGFRLKEHVLETLRGATSCEGKGWDKVTNPDAIIIDTFQVVRQDVSGFSPVL
                     TVNMRAASKSEPQTVVNASYSVTGFNL"
     gene            817..1224
                     /locus_tag="PN977_004184"
     CDS             817..1224
                     /locus_tag="PN977_004184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311708.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2509 family protein"
                     /protein_id="HDI6044160.1"
                     /translation="MNREKGGSSLALVLMLLILGSLLLQGMSQQDRSFASRVSMESQS
                     LRRQAIVQSTLEWGKMHSWQTQPAVQCLLYAATGARVCLRLLADNEALLIAGYEGVSL
                     WRTGEVIDGNIVFSPRGWSDFCPLKERALCQLP"
     gene            1209..1532
                     /locus_tag="PN977_004185"
     CDS             1209..1532
                     /locus_tag="PN977_004185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HDI6044161.1"
                     /translation="MSASLKNQQGFSLPEVMLAMVLMVMIVTALSGFQRTLMNSLASR
                     NQYQQLWRHGWQQTQLRAISPPANWQVNRMQTSQAGCVSINVTLVSPGGREGEMTRLH
                     CPNRQ"
     gene            1545..4913
                     /gene="recC"
                     /locus_tag="PN977_004186"
     CDS             1545..4913
                     /gene="recC"
                     /locus_tag="PN977_004186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HDI6044162.1"
                     /translation="MLRVYHSNRLDVLEALMEFIVERERLDDPFEPEMILVQSTGMAQ
                     WLQMTLSQKFGIAANIDFPLPASFIWDMFVRVLPEIPKESAFNKQSMSWKLMTLLPQL
                     LEREDFTLLRHYLTDDSDKRKLFQLSSKAADLFDQYLVYRLDWLAQWETGHLVEGLGE
                     AQAWQAPLWKALVEYTDELGQPRWHRANLYQRFIETLESATTCPPGLPSRVFICGISA
                     LPPVYLQALQALGKHIEIHLLFTNPCRYYWGDIKDPAYLAKLLTRQRRHSFEDRELPL
                     FRDSENAGQLFNSDGEQDVGNPLLASWGKLGRDYIYLLSDLEGSQELDAFVDVTPDNL
                     LHNIQSDILELENRAVAGVNIEEFSRSDNKRPLDPLDSSITFHVCHSPQREVEVLHDR
                     LLAMLEEDPTLTPRDIIVMVADIDSYSPFIQAVFGSAPADRYLPYAISDRRARQSHPV
                     LEAFISLLSLPDSRFVSEDVLALLDVPVLAARFDITEEGLRYLRQWVNESGIRWGIDD
                     DNVRELELPATGQHTWRFGLTRMLLGYAMESAQGEWQSVLPYDESSGLIAELVGHLAS
                     LLMQLNIWRRGLAQERPLEEWLPVCRDMLNAFFLPDAETEAAMTLIEQQWQAIIAEGL
                     GAQYGDAVPLSLLRDELAQRLDQERISQRFLAGPVNICTLMPMRSIPFKVVCLLGMND
                     GVYPRQLAPLGFDLMSQKPKRGDRSRRDDDRYLFLEALISAQQKLYISYIGRSIQDNS
                     ERFPSVLVQELIDYIGQSHYLPGDEALNCDESEARVKAHLTCLHTRMPFDPQNYQPGE
                     RQSYAREWLPAASQAGKAHSEFVQQLPFTLPETVPLETLQRFWAHPVRAFFQMRLQVN
                     FRTEDSEIPDTEPFILEGLSRYQINQQLLNALVEQDDAERLFRRFRAAGDLPYGAFGE
                     IFWETQCQEMQQLADRVIACRQPGQSMEIDLACNGVQITGWLPQVQSDGLLRWRPSLL
                     SVAQGMQLWLEHLVYCASGGNGESRLFLRKDGEWRFPPLAAEQALHYLSQLIEGYREG
                     MSAPLLVLPESGGAWLKTCYDAQNDAMLDDDSTLQKARTKFLQAYEGNMMVRGEGDDI
                     WYQRLWRQLTPETMEAIVEQSQRFLLPLFRFNQS"
     gene            5089..7977
                     /gene="ptrA"
                     /locus_tag="PN977_004187"
     CDS             5089..7977
                     /gene="ptrA"
                     /locus_tag="PN977_004187"
                     /EC_number="3.4.24.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin"
                     /protein_id="HDI6044163.1"
                     /translation="MPRSIWFKALLLFVALWAPLSQAETGWQPIQETIRKSDKDNRQY
                     QAIRLDNGMVVLLVSDPQAVKSLSALVVPVGSLEDPEAYQGLAHYLEHMSLMGSKKYP
                     QADSLAEYLKMHGGSHNASTAPYRTAFYLEVENDALPGAVDRLADAIAEPLLDKKYAE
                     RERNAVNAELTMARTRDGMRMAQVSAETINPAHPGSKFSGGNLETLSDKPGNPVQQAL
                     KDFHEKYYSANLMKAVIYSNKPLPELAKMAADTFGRVPNKESKKPEITVPVVTDAQKG
                     IIIHYVPALPRKVLRVEFRIDNNSAKFRSKTDELITYLIGNRSPGTLSDWLQKQGLVE
                     GISANSDPIVNGNSGVLAISASLTDKGLANRDQVVAAIFSYLNLLREKGIDKQYFDEL
                     ANVLDIDFRYPSITRDMDYVEWLADTMIRVPVEHTLDAVNIADRYDAKAVKERLAMMT
                     PQNARIWYISPKEPHNKTAYFVDAPYQVDKISAQTFADWQKKAADIALSLPELNPYIP
                     DDFSLIKSEKKYDHPELIVDESNLRVVYAPSRYFSSEPKADVSLILRNPKAMDSARNQ
                     VMFALNDYLAGLALDQLSNQASVGGISFSTNANNGLMVNANGYTQRLPQLFQALLEGY
                     FSYTATEDQLEQAKSWYNQMMDSAEKGKAFEQAIMPAQMLSQVPYFSRDERRKILPSI
                     TLKEVMAYRDALKSGARPEFMVIGNMTEAQATTLARDVQKQLGADGSEWCRNKDVVVD
                     KKQSVIFEKAGNSTDSALAAVFVPTGYDEYTSSAYSSLLGQIVQPWFYNQLRTEEQLG
                     YAVFAFPMSVGRQWGMGFLLQSNDKQPSFLWERYKAFFPTAEAKLRAMKPEEFAQIQQ
                     AVITQMLQAPQTLGEEASKLSKDFDRGNMRFDSRDKIVAQIKLLTPQKLADFFHQAVV
                     EPQGMAILSQISGSQNGKAEYVHPEGWKVWENVSALQQTMPLMSEKNE"
     gene            7970..11512
                     /gene="recB"
                     /locus_tag="PN977_004188"
     CDS             7970..11512
                     /gene="recB"
                     /locus_tag="PN977_004188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit beta"
                     /protein_id="HDI6044164.1"
                     /translation="MSDVAETLDPLRLPLQGERLIEASAGTGKTFTIAALYLRLLLGL
                     GGSAAFPRPLTVEELLVVTFTEAATAELRGRIRSNIHELRIACLRETTDNPLYERLLE
                     EIDDKAQAAQWLLLAERQMDEAAVFTIHGFCQRMLNLNAFESGMLFEQQLIEDESLLR
                     YQACADFWRRHCYPLPREIAQVVFETWKGPQALLRDINRYLQGEAPVIKAPPPDDETL
                     ASRHAQIVARIDTVKQQWRDAVGELDALIESSGIDRRKFNRSNQAKWIDKISAWAEEE
                     TNSYQLPESLEKFSQCFLEDRTKAGGETPRHPLFEAIDQLLAEPLSIRDLVITRALAE
                     IRETVAREKRRRGELGFDDMLSRLDSALRSESGEVLAAAIRTRFPVAMIDEFQDTDPQ
                     QYRIFRRIWHHQPETALLLIGDPKQAIYAFRGADIFTYMKARSEVHAHYTLDTNWRSA
                     PGMVNSVNKLFSQTDDAFMFREIPFIPVKSAGKNQALRFVFKGETQPAMKMWLMEGES
                     CGVGDYQSTMAQVCAAQIRDWLQAGQRGEALLMNGDDARPVRASDISVLVRSRQEAAQ
                     VRDALTLLEIPSVYLSNRDSVFETLEAQEMLWLLQAVMTPERENTLRSALATSMMGLN
                     ALDIETLNNDEHAWDAVVEEFDGYRQIWRKRGVMPMLRALMSARNIAENLLATAGGER
                     RLTDILHISELLQEAGTQLESEHALVRWLSQHILEPDSNASSQQMRLESDKHLVQIVT
                     IHKSKGLEYPLVWLPFITNFRVQDQAFYHDRHSFEAVLDLNAAPESVDLAEAERLAED
                     LRLLYVALTRSVWHCSLGVAPLVRRRGDKKGDTDVHQSALGRLLQKGEPQDAAGLRTC
                     IEALCDDDIAWQTAQTGDNQPWQVNDALTAELNARTLQRLPGDNWRVTSYSGLQQRGH
                     GIAQDLMPRLDVDAAGVVSVVEEPTLTPHQFPRGASPGTFLHSLFEDLDFTQPVDPNW
                     VQEKLELGGFEPQWEPVLTEWITAVLQAPLNETGVSLNQLSDRDKQVEMEFYLPISEP
                     LIASQLDALIRQFDPLSAGCPPLEFMQVRGMLKGFIDLVFRHEGRYYLLDYKSNWLGE
                     DSSAYTQQAMAAAMQAHRYDLQYQLYTLALHRYLRHRIADYDYERHFGGVIYLFLRGV
                     DKEHPQQGIYATRPNAGLIDLMDEMFAGMTLEEA"
BASE COUNT         2711 a         2858 c         3276 g         2696 t
ORIGIN      
        1 tcagggaggg gacgttatgg tgccttgtga gttccgctgc tggggccaat acctgtcatg
       61 gcagttcacc attggtcttt gtgccacgct ggcccgaagt cgaaatgagc gatctgacac
      121 cttcgcttgc tttctttggc ctgcgcaata ccgcatgggc cgggcatatt cgcttcaaaa
      181 actcaacggg cgagtggtgg ctggtggttt cgccgtgggg aagactccgg ctttgtcagc
      241 aaggagaaac agaaggatgc ctgtaaaaga gcaaggtttt tctctgctgg aagtgttgat
      301 tgctatggcg atcagtagcg tattgttgct gggggctgca cgctttctgc ctgcgttaca
      361 gcgtgaaagt ttaacgagca cccgtaagct ggcgctggaa gatgaaatct ggctgcgggt
      421 atttaccgtc gcgaagcatc tccagagggc gggttattgt catggcagct gtaccggcga
      481 agggctggaa attgtcggac agggtgactg tgtcattgtg cagtgggatg cgaacagtaa
      541 cggtatctgg gatcgcgaac cggtaaaaga gtccgaccag attggatttc gtctgaagga
      601 gcatgtgctg gaaacgctac gcggtgcgac atcctgtgaa ggtaagggct gggataaagt
      661 gactaatccg gatgccatca ttatcgacac ttttcaggtc gtacgtcagg atgtcagcgg
      721 cttctcgccg gtgttgacgg ttaatatgcg tgctgccagt aagtctgaac cgcaaaccgt
      781 ggtgaatgcc agctatagcg tgacaggatt caacctgtga accgcgaaaa ggggggctcg
      841 tcactggctc tggtcctgat gctgctgatt ttgggtagct tgctattaca aggaatgagt
      901 cagcaggatc gcagttttgc ctctcgcgtg agcatggaaa gtcagtcatt gcgccgccag
      961 gccatcgttc agtcgacgct ggagtgggga aaaatgcact cctggcagac acagcccgca
     1021 gttcagtgct tactgtacgc tgccaccggt gcccgggttt gtctgcgttt attggcagat
     1081 aatgaggctt tattgattgc cggttatgaa ggcgtttcgt tgtggcgaac aggcgaagtc
     1141 atcgatggaa acattgtttt ttcgccacgc ggctggagcg atttttgtcc gctgaaagag
     1201 agggcgttat gtcagcttcc ctgaagaatc aacaaggctt tagtctgccg gaggtaatgt
     1261 tggcgatggt gttgatggtg atgattgtca ctgcgttatc gggtttccag cgaacattaa
     1321 tgaacagtct tgccagcaga aaccagtacc aacagctctg gcggcatggc tggcagcaaa
     1381 cgcaactgcg tgcgatttcg ccacctgcca actggcaggt caaccgaatg cagacatcgc
     1441 aggcgggatg tgtcagcatc aacgttacgc tagtttcacc cgggggcaga gaaggcgaga
     1501 tgacccgcct gcattgcccg aatcgtcagt agtcaggagc cgctatgtta agggtctacc
     1561 attccaatcg tctggacgtg ctggaagcgt tgatggagtt tattgtcgaa cgcgaacggc
     1621 tggacgatcc tttcgaacca gagatgattc tggtgcaaag taccggtatg gcgcagtggc
     1681 tgcaaatgac cctgtcgcaa aagtttggta ttgcggcaaa cattgatttt ccgctgccag
     1741 cgagctttat ctgggatatg ttcgtccggg tgttaccgga aatccccaaa gagagcgcct
     1801 ttaacaaaca gagcatgagc tggaaactga tgactctgct gccgcaactg ttggagcgcg
     1861 aagactttac cctgttgcgg cattatctga ctgacgatag tgacaagcga aaactgttcc
     1921 agctttcttc aaaagcggcg gacctgtttg accagtatct ggtctatcgt ctggactggc
     1981 tggcacagtg ggaaacagga catctggtag aagggttggg agaagcacag gcctggcaag
     2041 cgccgttgtg gaaggcgttg gtggaatata ccgacgaact cgggcaaccg cgctggcacc
     2101 gcgccaatct ctatcagcgc tttatcgaaa cgctggagtc cgcgacgacc tgcccgccgg
     2161 ggctaccttc gcgcgtcttt atatgcggta tttccgcgtt accgcctgtt tatctccagg
     2221 cgctacaggc gctgggtaaa catattgaaa tccatctcct gtttaccaac ccctgccgtt
     2281 attactgggg cgacattaaa gatcctgctt atctggcgaa actgctgact cgccagcgcc
     2341 gacacagttt tgaagatcgc gaattaccgt tatttcgcga cagcgaaaat gccggacagc
     2401 tctttaacag cgatggtgaa caggatgtcg gtaacccgct gctggcctca tggggcaagc
     2461 ttgggcgcga ctacatttat cttctttctg acctggaggg cagccaggag ctggacgctt
     2521 ttgtcgatgt gacgccagac aacctgctgc ataacattca gtctgacatt ctggaactgg
     2581 aaaaccgcgc cgttgcgggt gtgaacatcg aagagttttc ccgtagcgat aacaaacgcc
     2641 cgcttgatcc actggatagc agtatcacct tccacgtttg ccatagcccg cagcgtgaag
     2701 ttgaagtttt acacgatcgc ttgctggcga tgctggagga agacccgaca cttactccgc
     2761 gcgacatcat cgtgatggtg gccgacatcg acagctacag tccgtttatt caggcagtgt
     2821 ttggtagcgc acctgcggat cgttacctgc cttacgccat ttccgaccgt cgtgcgcggc
     2881 agtcacatcc ggtactggaa gcgtttatca gcctgttatc actgcctgac agtcgctttg
     2941 tgtcagagga tgtgctggcg ctgctggatg tgccggtgct ggcggcgcgg tttgacatca
     3001 ccgaagaagg gctgcgttat ttacgtcagt gggtcaacga atctggcatt cgttggggga
     3061 tagatgacga caacgttcgc gagctggaac tccccgccac cggacaacac acctggcgat
     3121 ttggcctgac gcgtatgtta ctaggctacg cgatggagag cgcgcagggc gagtggcaat
     3181 cggttctacc ttatgatgaa tcgagcggct taattgcaga actggtgggg catctggctt
     3241 cactgctaat gcagctaaac atctggcgtc gcgggctggc gcaggagcgt ccgctggaag
     3301 agtggttgcc ggtttgtcgc gatatgctca acgccttctt cctgccggat gcggaaaccg
     3361 aagcggcgat gacgctgatc gaacaacaat ggcaggcgat tatcgccgaa ggtttaggtg
     3421 cgcagtatgg cgacgcggtg ccgctgtcac tattgcgtga tgaactggca cagcgcctgg
     3481 atcaggaacg tatcagccag cgttttctcg ccggaccggt taacatttgt actctgatgc
     3541 caatgcgttc catcccattc aaagtggttt gcctgctggg aatgaacgac ggcgtttatc
     3601 cacgccagct tgcgccattg ggctttgacc tgatgagcca gaaaccgaag cgtggcgacc
     3661 gtagccgtcg cgatgacgac cgctatctgt tcctggaagc gttaatttcc gcgcagcaaa
     3721 aactctatat cagctatatc gggcgttcta ttcaggataa cagtgaacgt ttcccgtcgg
     3781 tactggtgca ggaactgatc gactacatcg ggcaaagcca ttatctaccg ggcgatgaag
     3841 cgctcaactg tgatgaaagc gaggcaaggg taaaagcaca tcttacttgc ctccataccc
     3901 gaatgccgtt tgatccacaa aactaccagc caggcgaacg acaaagctat gctcgtgaat
     3961 ggctacccgc ggccagccag gctggtaaag cacattctga atttgttcag cagctgccgt
     4021 ttaccttacc ggaaaccgtg ccgctggaaa cgctacaacg attctgggca catccggtgc
     4081 gggcattttt ccagatgcgt ttgcaggtga acttccgtac cgaagacagc gaaatccccg
     4141 acaccgagcc atttattctg gaaggactta gccgttatca aatcaatcag cagttattga
     4201 atgcactggt tgagcaggat gatgccgaac gcttgttccg ccgcttccgg gcggcagggg
     4261 atttaccgta tggcgctttt ggtgaaattt tctgggaaac acagtgccag gagatgcagc
     4321 agcttgccga cagagtcatt gcctgtcgcc agccggggca gagtatggaa attgacctcg
     4381 cctgcaacgg tgtgcagata actggctggt tgccgcaggt gcagtcggat ggcctgttgc
     4441 gctggcgtcc ctctttatta agtgtggcgc agggaatgca actttggctg gaacaccttg
     4501 tctactgtgc cagcggtggt aatggtgaaa gtcgcctttt tctacgcaaa gacggcgagt
     4561 ggcgttttcc gccgcttgca gccgaacagg ctttacatta cctctcacag ctgattgaag
     4621 ggtatcgtga aggaatgtcc gcgccattgc tggtgttacc tgaaagtggc ggcgcgtggc
     4681 taaaaacctg ttatgacgcg caaaacgatg ccatgctgga tgacgattcc acgttgcaaa
     4741 aagcccgaac gaaattcctt caggcttacg aaggcaacat gatggtgcgt ggcgaaggtg
     4801 atgatatctg gtatcaacgg ctctggcggc aattaacacc agagacaatg gaggccatcg
     4861 ttgaacagtc gcaacgtttc ctgttaccgc tgtttcgctt taatcagtca tgagtgctgt
     4921 ataaaaattg cgcaatatat ctgcttgctt tatgatgcgc accagtcacg gactgatgga
     4981 tatatagaca taggctgact cgtgcagcac aagattaaat tctggcagat gatttgcgtt
     5041 aacctgttga atctggccag aaaataaagt tgattatgag gtccgtgaat gccccgcagc
     5101 atctggttca aagcattatt gttgtttgtt gccctttggg cacccttaag tcaggcagaa
     5161 acgggatggc agccgattca ggaaaccatc cgtaaaagtg ataaagataa ccgccagtat
     5221 caggctatac gtctggataa cggtatggtg gtcttgctgg tttctgatcc gcaggcggtt
     5281 aaatcgctct cggcgctggt ggtgcccgtt gggtcgctgg aagatcccga ggcgtatcag
     5341 gggctggcgc attaccttga acacatgagt ctgatggggt cgaaaaagta cccgcaggct
     5401 gacagtctgg ccgaatatct caaaatgcac ggcggcagtc acaatgccag cacggcaccg
     5461 tatcgcacgg ctttctatct ggaagttgag aacgacgcct tgcccggtgc ggtagaccgc
     5521 ctggcggacg ctattgcaga acccttgctc gacaagaaat acgccgaacg tgaacgtaat
     5581 gcagtgaatg ccgaattaac catggcgcgt acgcgtgacg ggatgcgcat ggcacaggtc
     5641 agcgcagaaa ccattaaccc ggcacacccc ggttcaaagt tttctggtgg taacctcgaa
     5701 accttaagcg acaaaccagg taatccggta cagcaggcgc tgaaagattt ccacgagaag
     5761 tactattccg ccaatctgat gaaggcggtt atttacagta ataaaccgtt gccggagttg
     5821 gcaaaaatgg cggcggacac ctttggtcgc gtgccgaaca aagagagcaa aaaaccggaa
     5881 atcaccgtgc cggtagtcac cgacgcgcaa aagggcatta tcattcatta cgtcccggcg
     5941 ttgccgcgta aagttctgcg cgttgagttt cgcatcgata acaattcagc gaagttccgt
     6001 agtaaaacgg atgaattgat tacctatctg attggtaatc gcagcccagg tacactttct
     6061 gactggctgc aaaagcaggg attagttgag ggtattagcg ccaattccga tcctatcgtc
     6121 aacggcaaca gcggcgtatt agcgatctct gcgtctttaa ccgataaagg tctggcgaat
     6181 cgcgatcagg ttgtggcggc catttttagc tacctcaatc tgttacgcga aaaagggatt
     6241 gataaacaat acttcgatga actggcgaat gtgctggata tcgacttccg ttatccgtca
     6301 atcacccgtg atatggatta cgtcgaatgg ctggcagata ccatgattcg cgttcctgtt
     6361 gagcatacgc tggatgcagt caatattgcc gatcggtacg acgctaaagc agtaaaagaa
     6421 cgcctggcga tgatgacgcc gcagaatgcg cgtatctggt atatcagccc gaaagagccg
     6481 cacaacaaaa cggcttactt tgtcgatgcg ccgtatcagg tcgataaaat tagcgcacaa
     6541 actttcgctg actggcagaa aaaagccgcc gacattgcgc tctctttgcc agagcttaac
     6601 ccctatattc ctgacgattt ctcgctgatt aagtcagaga agaaatatga ccatccagag
     6661 ctgattgttg atgagtcgaa tctgcgtgtg gtgtatgcgc caagccgtta tttttccagc
     6721 gaacccaaag ctgatgtcag cctgattttg cgtaatccaa aagccatgga tagcgcccgc
     6781 aatcaggtga tgtttgcgct caatgattat ctcgcagggc tggcgcttga tcagttaagc
     6841 aaccaggcgt cggttggtgg cataagtttt tccaccaatg ctaacaacgg ccttatggtt
     6901 aatgccaatg gttacaccca gcgcctgccg cagctgttcc aggcattgct cgaggggtac
     6961 tttagctata ccgctacgga agatcagctt gagcaggcga agtcctggta taaccagatg
     7021 atggattctg cagaaaaggg caaagcgttc gagcaggcga ttatgcccgc gcagatgctc
     7081 tcgcaagtac catacttctc gcgagatgaa cggcgcaaaa ttttgccctc cattactttg
     7141 aaagaggtga tggcctatcg cgacgcctta aaatcagggg ctcgaccaga gtttatggtt
     7201 atcggcaaca tgaccgaggc ccaggcaaca acgctggcac gcgatgtgca aaaacagttg
     7261 ggcgctgatg gttcggagtg gtgtcgtaac aaagatgtcg tggtcgataa aaaacaatcc
     7321 gtcatctttg aaaaagccgg taacagcacc gactccgcac tggcagcggt atttgtaccg
     7381 actggctacg atgaatacac cagttcagcg tatagttctc tgttggggca gatcgtacag
     7441 ccgtggttct acaatcagtt gcgtaccgaa gaacagttgg gctatgccgt gtttgcgttt
     7501 ccaatgagcg tggggcgtca gtggggcatg ggcttcctgt tgcaaagcaa tgataaacag
     7561 ccttcattct tgtgggagcg ttacaaggcg tttttcccga ccgcagaagc gaaactgcgg
     7621 gcgatgaagc cagaagagtt tgcgcaaatc cagcaggcgg taattaccca gatgctgcag
     7681 gcaccgcaaa cgctcggcga agaagcatcg aagttaagta aagatttcga tcgcggcaat
     7741 atgcgcttcg attcgcgtga taaaatcgtg gcccagataa aactgctgac gccgcaaaaa
     7801 cttgctgatt tcttccatca ggcggtggtc gagccgcaag gtatggctat tctgtcgcag
     7861 atttccggca gccagaacgg gaaagccgaa tatgtgcatc ctgaaggctg gaaagtgtgg
     7921 gagaacgtca gcgcgttgca gcaaacaatg cccctgatga gtgaaaagaa tgagtgatgt
     7981 cgccgagaca ctagatcctt tgcgcttgcc cttacagggc gagcgcctga ttgaagcctc
     8041 tgccggcaca ggcaaaacct ttacgattgc ggcgctctat ttgcgcctgt tacttggact
     8101 aggcggttct gccgcctttc cccgcccgct gaccgttgaa gaactgctgg tggtcacctt
     8161 taccgaggct gccacggcag aattgcgcgg tcgtatccgt agcaatatcc acgagttgcg
     8221 catcgcctgt ctgcgtgaaa ccaccgacaa tccactgtac gaacgcctgc tggaagagat
     8281 cgacgataaa gcgcaagccg cgcagtggtt gttgttagcc gagcggcaga tggatgaagc
     8341 ggcagtcttt actattcacg gcttttgcca gcgcatgctc aacctgaatg cctttgaatc
     8401 cggcatgctg tttgagcagc agctgattga agatgagtct ctgctacgct accaggcctg
     8461 cgccgatttc tggcgtcgcc actgctaccc gctgccgcgt gaaattgccc aggtcgtctt
     8521 tgaaacctgg aaagggccgc aggcgttact gcgcgatatt aatcgttatc tgcaaggcga
     8581 agcgccggtt atcaaagcgc cgccgcccga tgatgaaacg ctggcttccc gccacgcgca
     8641 aattgtggcg cgtattgata cggtaaaaca gcagtggcgc gacgcggtgg gtgaactgga
     8701 tgcgctgatc gaatcttctg gtattgatcg acgcaagttt aaccgtagca atcaggctaa
     8761 atggatcgac aagatcagcg cctgggcaga agaagagacc aacagctatc agttgccaga
     8821 gtcgctggaa aaattctctc agtgtttctt agaagatcgc acgaaagccg ggggggaaac
     8881 cccgcgacat ccactgtttg aggcgatcga tcaactgctt gcagaaccat tgtcgatccg
     8941 cgatctggtg atcactcgcg cattagctga gatccgcgaa acagtagcgc gtgaaaaacg
     9001 ccgccgtggc gaattgggtt ttgatgacat gttaagtcgg ctcgattccg cgctgcgtag
     9061 tgaaagcggc gaggtattgg cagcggcgat ccgtacgcga ttcccggtgg caatgatcga
     9121 tgaatttcag gataccgacc cccagcagta ccgaattttt cgccgtatct ggcaccatca
     9181 gccggaaacc gcattgttgc tgattggcga cccgaaacag gccatttatg cattccgggg
     9241 tgcggatatc ttcacttata tgaaggcgcg tagcgaagtt cacgcccact acaccttaga
     9301 caccaactgg cgttccgcac cgggaatggt gaacagcgtt aacaaacttt tcagccagac
     9361 tgatgacgcg ttcatgtttc gtgaaatacc gtttattcct gtgaaatctg ccgggaaaaa
     9421 tcaggcgtta cgttttgtgt ttaaaggtga aacgcagcct gcgatgaaaa tgtggctgat
     9481 ggaaggcgaa agctgcggcg ttggcgacta tcaaagtacc atggcgcagg tatgtgctgc
     9541 gcaaatccgc gactggttac aggctgggca gcgcggggaa gcgttgctga tgaacggcga
     9601 tgatgcacgt ccggtacgtg cttcggacat cagtgtgctg gtgcgcagcc gccaggaggc
     9661 cgctcaggtg cgcgatgcct taacgctgct ggaaatccct tccgtttacc tttcgaaccg
     9721 cgacagtgtt tttgaaactc tggaagcaca ggagatgctt tggctgttgc aggcggtgat
     9781 gacacccgaa cgtgagaaca ccctgcgcag tgcgctggca acgtcaatga tggggctgaa
     9841 tgcgctggat attgaaacgc tgaataatga cgaacatgcg tgggatgcgg tagtcgaaga
     9901 gttcgatggt tatcggcaaa tctggcgcaa acgtggcgtt atgccgatgc tgcgggcgct
     9961 gatgtcggcg cgtaacattg cagaaaactt gctggcaaca gcaggcggtg agcggcgtct
    10021 taccgatatc ttgcatatca gcgaattgct acaagaagcc ggcacgcagc tggaaagtga
    10081 acatgcgctg gtacgctggt tatcgcaaca tatcctcgag ccagacagta atgcctccag
    10141 ccaacaaatg cgtctcgaaa gtgataaaca tctcgtgcag attgtcacga tccacaaatc
    10201 gaaagggctg gaatatccat tggtctggct gccgtttatc accaatttcc gcgtccagga
    10261 tcaggcgttt tatcacgatc gccactcgtt tgaggctgtt ctggatctta atgctgcgcc
    10321 agaaagcgtc gacctcgcgg aggccgaacg tctggcggaa gatctgcgtt tgctttacgt
    10381 ggcgctgacg cgttcggttt ggcattgcag tctcggcgtt gcaccgctgg tgcgccgtcg
    10441 tggcgataaa aaaggtgata ccgacgtcca ccaaagtgcg ctcgggcgtt tgctgcaaaa
    10501 aggggaaccg caagatgcgg cagggcttcg cacctgtatt gaagcgttat gcgatgatga
    10561 tattgcctgg caaacggcac aaactggtga taaccagccc tggcaggtta atgatgcatt
    10621 aactgcagaa ctgaatgcga ggacgctaca acgattgccc ggcgataact ggcgtgtcac
    10681 cagctactcc ggtttgcagc agcgtggtca cggtatcgct caggatctga tgccacggtt
    10741 ggatgttgat gccgcaggcg tggtcagcgt cgttgaagaa ccgacgttaa caccgcatca
    10801 gttcccgcgc ggtgcgtcac cggggacatt cttgcacagt ttgtttgaag acctcgattt
    10861 tacccagccg gttgacccga actgggtaca ggaaaaactg gagctcggtg gctttgaacc
    10921 gcagtgggaa ccggtgttga ctgagtggat cacggctgtc ctccaggcac ctctcaatga
    10981 aacgggtgtt agcctgaatc agctttccga tcgcgataaa caggtggaga tggagtttta
    11041 cctgccgatt agtgaaccgc ttatcgccag ccagcttgat gcactaattc gccagtttga
    11101 cccgctatcc gctggctgcc cgccgctgga gttcatgcag gtacgcggca tgttaaaagg
    11161 ctttatcgac ctggtattcc gccacgaagg gcgttattac ctgctcgact ataaatccaa
    11221 ctggttgggt gaagacagtt cggcttacac ccaacaggct atggcagcgg caatgcaggc
    11281 acaccgctat gatctgcaat atcagcttta taccctggcg ctgcaccgtt atctgcgcca
    11341 tcgcattgct gattatgact atgagcgcca ctttggcggc gttatttatc tgttcctgcg
    11401 tggcgttgat aaagaacatc cgcaacaagg gatctacgcg acccgaccca acgccgggtt
    11461 gattgacctg atggatgaga tgtttgccgg tatgaccctg gaggaggcgt aatgaaattg
    11521 caaaagcaat tactggaagc t
//
LOCUS       DAMZFT010000147        11480 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.147, whole genome shotgun sequence.
ACCESSION   DAMZFT010000147 DAMZFT010000000
VERSION     DAMZFT010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11480)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11480)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11480
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.147"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..395
                     /gene="rnk"
                     /locus_tag="PN977_004189"
     CDS             <1..395
                     /gene="rnk"
                     /locus_tag="PN977_004189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="nucleoside diphosphate kinase regulator"
                     /protein_id="HDI6044165.1"
                     /translation="IINDLDAERIDILLEQPAYAGLPIADALNAELDRAQMCSPEEMP
                     HDVVTMNSRVKFRNLSDGEVRVRTLVYPAKMTDSNTQLSVMAPVGAALLGLRVGDSIH
                     WELPGGVATHLEVLELEYQPEAAGDYLL"
     gene            751..1179
                     /gene="uspG"
                     /locus_tag="PN977_004190"
     CDS             751..1179
                     /gene="uspG"
                     /locus_tag="PN977_004190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspG"
                     /protein_id="HDI6044166.1"
                     /translation="MYKTIIMPVDVFEMELSDKAVRHAEFLAQDDGVIHLLHVLPGSA
                     SLSLHRFAADVRRFEEHLQHEAEERLQTMVSHFTIDPSRIKQHVRFGSVRDEVNELAK
                     ELDADVVVIGSRNPSISTHLLGSNASSVIRHANLPVLVVR"
     gene            complement(1300..2865)
                     /gene="ahpF"
                     /locus_tag="PN977_004191"
     CDS             complement(1300..2865)
                     /gene="ahpF"
                     /locus_tag="PN977_004191"
                     /EC_number="1.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017455864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HDI6044167.1"
                     /translation="MLDTNMKTQLKAYLEKLTKPVELIATLDDSAKSAEIKELLAEIA
                     ELSDKVTFKEDNSLPVRKPSFLITNPGSNQGPRFAGSPLGHEFTSLVLALLWTGGHPS
                     KEAQSLLEQIRHIDGDFEFETYYSLSCHNCPDVVQALNLMSVLNPRIKHTAIDGGTFQ
                     NEITDRNVMGVPAVFVNGKEFGQGRMTLTEIVAKIDTGAEKRAAEELNKRDAYDVLIV
                     GSGPAGAAAAIYSARKGIRTGLMGERFGGQILDTVDIENYISVPKTEGQKLAGALKVH
                     VDEYDVDVIDSQSASKLIPAAVEGGLHQIETASGAVLKARSIIVATGAKWRNMNVPGE
                     DQYRTKGVTYCPHCDGPLFKGKRVAVIGGGNSGVEAAIDLAGIVEHVTLLEFAPEMKA
                     DQVLQDKLRSLKNVDIILNAQTTEVKGDGSKVVGLEYRDRVSGDIHNIELAGIFVQIG
                     LLPNTNWLEGAVERNRMGEIIIDAKCETNVKGVFAAGDCTTVPYKQIIIATGEGAKAS
                     LSAFDYLIRTKTA"
     gene            complement(2994..3557)
                     /gene="ahpC"
                     /locus_tag="PN977_004192"
     CDS             complement(2994..3557)
                     /gene="ahpC"
                     /locus_tag="PN977_004192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003858764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit C"
                     /protein_id="HDI6044168.1"
                     /translation="MSLINTKIKPFKNQAFKNGEFIEITEKDTEGRWSVFFFYPADFT
                     FVCPTELGDVADHYEELQKLGVDVYAVSTDTHFTHKAWHSSSETIAKIKYAMIGDPTG
                     ALTRNFDNMREDEGLADRATFVVDPQGIIQAIEVTAEGIGRDASDLLRKIKAAQYVAS
                     HPGEVCPAKWKEGEATLAPSLDLVGKI"
     gene            3929..4675
                     /gene="dsbG"
                     /locus_tag="PN977_004193"
     CDS             3929..4675
                     /gene="dsbG"
                     /locus_tag="PN977_004193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308670.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbG"
                     /protein_id="HDI6044169.1"
                     /translation="MLKKILLLALLPAIAFAEELPAPVKAIEKQGITIIKTFDAPGGM
                     KGYLGKYQDMGVTIYLTPDGKHAISGYMYNEKGENLSNTLIEKEIYAPAGREMWQRME
                     QSHWLLDGKKDAPVIVYVFADPFCPYCKQFWQQARPWVDSGKVQLRTLLVGVIKPESP
                     ATAAAILASKDPAKTWQEYEASGGKLKLNVPANVSTEQMKVLSDNEKLMDDLGANVTP
                     AIYYMSKENTLQQAVGLPDQKTLNIIMGNK"
     gene            4880..5782
                     /locus_tag="PN977_004194"
     CDS             4880..5782
                     /locus_tag="PN977_004194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDI6044170.1"
                     /translation="MANLYDLKKFDLNLLVIFECIYQHLSISKAAESLYITPSAVSQS
                     LQRLRAQFNDPLFIRSGKGIAPTTTGLNLHHHLEKNLRGLEQTINIVNKSELKKNFII
                     YGPQLISCTNNNMLIRCLRQDTSIDIECHDILLSAENAEELLVQRKTDLAITLQPVIS
                     RSVICMPLHTIRNSLICSNRHPRITDTSNYEQIIAEEFTLLISKSAGIDEIQMDIDER
                     FMNRKVSFRSSSLMTIINSISVTDLLGIVPTELYNLHRDFLKLKEIKLEQPLPAVKLY
                     ISYNKASLNNLVFSRFIDRLNDSF"
     gene            5929..7149
                     /locus_tag="PN977_004195"
     CDS             5929..7149
                     /locus_tag="PN977_004195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HDI6044171.1"
                     /translation="MSIYKIPLPLNILEAAKERITWTLNTLPRICVSFSGGKDSGLML
                     HLTAEIARQMGKKICVLFIDWEAQFSCTINYVQSLREFYADVIEEFYWVALPLTTQNS
                     LSQYQPEWQCWEPDVEWVRQPPQDAITDPDFFSFYQPGMTFEQFVREFAEWFSQKRPA
                     AMMIGIRADESYNRFVAIASLNKQRFADDKPWTTAAPGGHSWYIYPIYDWKVADIWTW
                     YANHQQLCNPLYNIMYQAGVPLRHMRICEPFGPEQRQGLWLYHVIEPDRWAAMCARVS
                     GVKSGGIYAGHDNHFYGHRKILKPEHLDWQEYALLLLNSMPEKTAEHYRNKIAIYLHW
                     YQKKGIEVPQTQQGDIGAKDIPSWRRICKVLLNNDYWCRALSFSPTKAKNYQRYNERI
                     KGKRQEWGILCNND"
     gene            7122..7751
                     /locus_tag="PN977_004196"
     CDS             7122..7751
                     /locus_tag="PN977_004196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB-like nuclease domain-containing protein"
                     /protein_id="HDI6044172.1"
                     /translation="MGDTMQQRLTQDLTQFLASLPEDDRIKAINEIRMAIHQVSPFRE
                     EPVDCVLWVKNSQLMPNDYNPNNVAPPEKKLLKKSIEIDGFTQPIVVTHTDKNALEIV
                     DGFHRHEIGKGSSSLKLRLKGYLPVTCLEGTRNQRIAATIRHNRARGRHQITAMSEIV
                     RELSQLGWDDNKIGKELGMDSDEVLRLKQINGLQELFADRQFSRAWTVK"
     gene            complement(7752..8912)
                     /gene="ybdL"
                     /locus_tag="PN977_004197"
     CDS             complement(7752..8912)
                     /gene="ybdL"
                     /locus_tag="PN977_004197"
                     /EC_number="2.6.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine-oxo-acid transaminase"
                     /protein_id="HDI6044173.1"
                     /translation="MTNNPLIPQSKLPQLGTTIFTQMSALAQQHQAINLSQGFPDFDG
                     PRYLQERLAYHVAQGANQYAPMTGVQALREAIAQKTERLYGYQPDADSDITVTAGATE
                     ALYAAITALVRNGDEVICFDPSYDSYAPAIALSGGIVKRIALQPPHFRVDWQEFAALL
                     SERTRLVILNTPHNPSATVWQQADFSALWQAIAGHEIFVISDEVYEHINFSQQGHASV
                     LAHPQLCERAVAVSSFGKTYHMTGWKVGYCVAPAPISAEIRKVHQYLTFSVNTPAQLA
                     LADMLRAEPEHYLALPDFYRQKRDILVNALNESRLEILPCEGTYFLLVDYSAVSTLDD
                     VEFCQWLTREHGVAAIPLSVFCADPFPHKLIRLCFAKKESTLLAAAERLRQL"
     gene            9021..10109
                     /gene="hcxA"
                     /locus_tag="PN977_004198"
     CDS             9021..10109
                     /gene="hcxA"
                     /locus_tag="PN977_004198"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcxA"
                     /protein_id="HDI6044174.1"
                     /translation="MPHNPIRVVVGPANYFSHPGSFNHLHDFFTDEQLSRAAWIYGER
                     AIAAAQTKLPPAFELPGAKHILFRGHCSESDVQQLAAESGDDRSVVIGVGGGALLDTA
                     KALARRLGLPFVAVPTIAATCAAWTPLSVWYNDAGQALHYEIFDDANFMVLVEPEIIL
                     NAPQEYLLAGIGDTLAKWYEAVVLAPQPETLPLTVRLGINNAQAIRDVLLNSSEQALA
                     DQQNHQLTQSFCDVVDAIIAGGGMVGGLGDRFTRVAAAHAVHNGLTVLPQTEKFLHGT
                     KVAYGILVQSALLGQDDVLAQLTGAYQRFHLPTTLAELEVDINNQAEIDKVIAHTLRP
                     VESIHYLPVTLTPDTLRAAFEKVESFKA"
     gene            complement(10119..10316)
                     /locus_tag="PN977_004199"
     CDS             complement(10119..10316)
                     /locus_tag="PN977_004199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HDI6044175.1"
                     /translation="MFDSLAKAGKYLGQAAKLMIGMPDYDNYVEHMRVNHPDQTPMTY
                     EEFFRERQDARYGGKGGARCC"
     gene            complement(10429..>11480)
                     /locus_tag="PN977_004200"
     CDS             complement(10429..>11480)
                     /locus_tag="PN977_004200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="carbon starvation protein A"
                     /protein_id="HDI6044176.1"
                     /translation="MLANEGQACFIGYGGMLMESFVAIMALVSACIIDPGVYFAMNSP
                     MAVLAPAGTADVVASAAQVVSSWGFAITPDTLNQIASEVGEQSIISRAGGAPTLAVGM
                     AYILHGALGGMMDVAFWYHFAILFEALFILTAVDAGTRAARFMLQDLLGVVSPGLKRT
                     DSLPANLLATALCVLAWGYFLHQGVVDPLGGINTLWPLFGIANQMLAGMALMLCAVVL
                     FKMKRQRYAWVALVPTAWLLICTLTAGWQKAFSLDAKVGFLAIANKFQAMIDSGNIPS
                     QYTESQLAQLVFNNRLDAGLTIFFMVVVVVLALFSIKTALAALKEPKPTAKETPYEPM
                     PENVEEIVAQAKGAH"
BASE COUNT         2945 a         2950 c         2719 g         2866 t
ORIGIN      
        1 tcatcattaa cgacctggat gccgaacgca tcgatattct gctggagcaa cccgcctatg
       61 ctggtttgcc aatcgccgac gcgttaaacg cagagttgga tcgcgcccaa atgtgttcgc
      121 cagaagagat gccacacgac gtggtgacaa tgaacagccg ggttaaattc cgcaatctta
      181 gcgatggcga agtgcgtgtg cgcacgctgg tgtatccggc aaaaatgacc gacagcaata
      241 ctcagctttc cgttatggct ccggtaggtg ccgcactgct ggggctgcgc gttggcgatt
      301 ccattcactg ggaactgccg ggcggcgttg caacccacct tgaagtgctg gaactcgaat
      361 accagccaga agctgctggc gattacctgc tttaatccca tctgcacaag cgataactcg
      421 ctttgcccag aggatgcact cgcatcctct ttccgctcaa atatcaacca gatttatcat
      481 tttgctctta agcgcagcat tctaatctga actggttatt catctgcgga acctcatatg
      541 agcgaagctc tcggtatcac tgcattttat atattcatag cagcaattat tgttgctgca
      601 gtgctatacc ttgaacaacg ctggtagcgc ctggttttat ttattttcca gctcaatagc
      661 aaattcagat caaatcagcc tcgcaaatca tagattcata accaataatg tgctgttatg
      721 tatacatgca aaaacaagga gagaatgctt atgtacaaaa caatcatcat gccagtggat
      781 gttttcgaaa tggaattgag cgacaaagcg gttcgccacg ctgaattcct cgcccaggat
      841 gacggagtta ttcatctact tcacgtacta ccggggtcag ccagcctgag cctgcaccgt
      901 tttgccgctg atgtacgtcg ttttgaagag catctgcaac atgaagcaga agaacgtctg
      961 caaacgatgg tcagccactt caccatcgat ccttcccgca ttaagcaaca tgtccgtttt
     1021 ggtagtgtgc gggatgaagt taacgagtta gcgaaagaac ttgatgcaga tgtagtagtg
     1081 attggttcgc gtaacccatc aatttcaact catctgttgg gttctaacgc ctcgagcgtg
     1141 atccgccacg ccaatttgcc ggtgctggtt gtgcgttaat tgccagaata agtatcccgc
     1201 cctgcccgga gccatccctg cagggctttt taatgcaata aaaaagccgc caggtttgac
     1261 cccggcggct aagcaattgc aggtgaatct tacttcttct tatgcagttt tggtgcgaat
     1321 caggtagtca aaagcactca gagaggcttt ggcaccttcg ccagtagcga tgatgatctg
     1381 cttgtacgga accgtcgtac agtcacccgc tgcgaacacg cctttgacgt tggtttcgca
     1441 tttcgcatca atgataatct cgcccatgcg gttacgttcg actgcgcctt ccagccagtt
     1501 ggtgttcggc agcagaccaa tctggacgaa aataccggcc agttcgatgt tgtgaatatc
     1561 gccgctgaca cgatcgcgat attccagacc tacgactttg ctaccgtcgc ctttcacttc
     1621 cgtggtttgc gcattcagaa taatgtcgac gtttttcagg ctgcgcagtt tgtcctgcag
     1681 aacctggtcg gctttcattt ctggcgcaaa ttccagcagc gttacgtgct caacgatacc
     1741 cgccaggtca attgccgctt ccacgccgga gttaccgccg ccgataaccg ctacgcgttt
     1801 gcctttaaac agcgggccgt cgcagtgcgg gcagtaggtc acgcctttgg tgcgatactg
     1861 atcttcgcca ggaacgttca tgttgcgcca ttttgcaccg gtcgccacga taatgctgcg
     1921 tgctttcagt accgcgccag aagctgtttc aatctgatgc aggccgcctt caaccgctgc
     1981 cgggatcagt ttgctcgcgc tctggctgtc gatcacatca acgtcgtatt catcaacatg
     2041 aactttcagc gcacctgcca gtttctggcc ttcggtcttc ggtacagaaa tgtagttttc
     2101 gatatcaacg gtatcgagga tctgaccacc aaaacgttcg cccatcagac cggtacggat
     2161 gcctttacgt gcggagtaaa ttgctgccgc tgcacccgcc gggccggaac caacgattaa
     2221 tacgtcataa gcatcacgct tgttcagctc ttctgccgca cgtttttccg cacccgtatc
     2281 aattttggca acgatttcag tcaacgtcat gcggccctga ccaaactctt tcccgtttac
     2341 gaacactgcc ggaacgccca tcacgttgcg atcggtgatt tcgttctgga aggtgccgcc
     2401 gtcaattgca gtgtgcttga tgcgcgggtt cagtacgctc atcaggttca gcgcctgcac
     2461 cacgtccggg cagttgtggc aagagagcga gtaataggtt tcgaattcaa aatcaccgtc
     2521 aatatggcga atctgctcca gcagagactg cgcttctttc gacggatgac caccggtcca
     2581 cagcaacgcc agtaccagcg aggtgaactc gtggcccagc ggggagcctg caaaacgtgg
     2641 tccctggttg gaacctgggt tggtgatcag gaaagacggc ttacgcactg gcaagctgtt
     2701 atcttcttta aaggtgactt tgtctgacag ttctgcgatt tcagccaaca gttccttgat
     2761 ttctgccgat ttagcgctgt catccagcgt ggcaattaac tcaacaggct tggtcaattt
     2821 ctcaaggtaa gccttgagtt gagttttcat atttgtgtcg agcatgttta tctcctgggc
     2881 tttaaacatc atcatgcaag cggcctctgc ggctacctgg atgcagcttg catcatggtg
     2941 cggggaagaa aatcgggtgc cgcagcaccc gattaattac gcgattgcgg aacttagatt
     3001 ttaccaacca ggtccagaga cggagccaga gttgcttcac cttctttcca tttagccggg
     3061 caaacttcac ctgggtgaga agctacgtac tgtgctgctt tgattttacg cagcaggtca
     3121 gacgcgtcac ggccaatgcc ttcagcggta acttcgattg cctggatgat accctgcggg
     3181 tcaacaacga aggtcgcacg gtcagccaga ccttcatctt cacgcatgtt gtcgaagtta
     3241 cgggtcaggg cgccagtcgg gtcgccgatc atcgcatatt tgattttagc gatggtttca
     3301 gagctgctgt gccatgcttt gtgggtgaag tgagtatcgg tagatactgc gtatacgtct
     3361 acgcccagtt tctgcagttc ttcgtaatgg tcagcaacgt cacccagttc ggtcgggcat
     3421 acgaaagtaa agtcagccgg gtagaagaag aagacgctcc agcggccttc ggtatctttt
     3481 tcggtgattt cgatgaattc gccgtttttg aatgcctggt ttttaaaagg tttaattttg
     3541 gtgttaatca aggacatcta tacttcctcc gtgttttcga tgagatgtaa ggtaaccaat
     3601 ttttgccgat tcggctaatg cgtttccatt atcaaatcga taagttttac cttacaacct
     3661 tcgtaagaca actttgtgaa ctttgcaccg ccagcggcga taaaaagtaa aatggccccc
     3721 tttccaggtg accgtgtaat ctgaactacc ctttggtatc ttcagcgcct gcctgcaggc
     3781 tgaggtgttg cccttacaaa tgcaacaacg acgtggatta caacaccctc ataaacaaag
     3841 ggcaatcacc tgatctaagc tcttacctat gacagtgata ggttatgcct tttacccgac
     3901 ttttgcactg actgaaaagg acaaattaat gttaaaaaag atacttttac tggctctgct
     3961 tcctgcaatc gccttcgcag aggaacttcc tgccccagta aaagcgattg aaaaacaggg
     4021 cattacaatc atcaaaacgt tcgatgctcc cggaggaatg aaaggttatc tcggcaagta
     4081 tcaggatatg ggcgtcacca tctacctgac tccagatggt aagcacgcta tctccggtta
     4141 catgtacaac gagaaaggtg aaaacctgag taacacactt atcgaaaaag aaatttacgc
     4201 acccgccgga cgcgaaatgt ggcaacggat ggaacaatcc cactggctcc tcgacggtaa
     4261 aaaagacgcg ccggtcattg tctacgtctt cgccgatccg ttctgcccat attgtaaaca
     4321 gttctggcag caggctcgcc cgtgggtaga ttctggcaaa gtgcaattaa gaacattgtt
     4381 ggttggcgtt atcaagccag aaagcccggc gacagcagcg gcaattcttg cctccaaaga
     4441 tcccgcaaaa acctggcaag aatatgaagc ctctggtggc aagcttaagc taaacgtgcc
     4501 tgcaaacgta agtacagagc aaatgaaagt gttaagcgat aacgagaaac tgatggacga
     4561 tctgggggca aatgtcacgc cagctatcta ttacatgagt aaggaaaata cactgcaaca
     4621 ggccgtgggg ttgcccgatc agaaaacgct taatatcatt atggggaata aataaaagat
     4681 aattcatgga tggttgaaat atattcctgc ttttcaacca tcctaaaaaa gcatgaattg
     4741 ctcgtaaccc acctgacagc tatgctattt tagaaaatct taatccaaag ttaagcagaa
     4801 aaacataaca catataaata caacaacatt attgatcaag tgaatgattt atggaagaga
     4861 caatacaacg gagtatgtta tggctaatct ttatgatcta aaaaaatttg acctcaattt
     4921 actggttata tttgaatgta tttatcaaca tctcagcatt agtaaagcgg cagaatcatt
     4981 atatataacg ccctctgctg ttagtcagtc gttacaacgt ttacgtgcgc aatttaatga
     5041 cccgttattc attcgctcag ggaaaggtat cgcgccgact acaacagggt taaacctgca
     5101 tcaccatctt gagaaaaatc tcagaggcct tgaacaaacg atcaatatcg ttaataagtc
     5161 tgaactaaag aaaaatttta ttatctacgg tcctcagctt atttcttgta ctaataacaa
     5221 catgctaatc cgctgtctta gacaagatac ttcaatcgat atagaatgcc atgacatact
     5281 gctttcagct gaaaatgccg aagaactgtt ggttcaacgc aaaactgacc tggcaattac
     5341 actacaacca gtaattagcc gctctgtcat ctgcatgccg ttacatacta ttcgcaattc
     5401 tcttatatgt agcaacagac atcctcgtat taccgatacc agtaactatg aacaaattat
     5461 cgccgaagaa ttcaccctgt tgatatctaa atcagcaggg attgacgaaa ttcaaatgga
     5521 catagatgaa cgttttatga accgtaaagt ttcatttcgc agttcttctt tgatgactat
     5581 aattaatagt atttctgtta ctgacttatt aggcattgtg cccaccgaac tgtacaattt
     5641 acatcgtgac tttttgaagt taaaagaaat aaaactggaa caacctcttc ctgcggttaa
     5701 actttatatt tcctataaca aggcttcgtt gaacaacctt gtcttctccc gattcataga
     5761 tcgtttaaat gatagcttct gattcattac cactgacaaa taatatatta cgcatcaatt
     5821 aattcattat gtgaatataa aataatgtgc tctatcttgc ttcgtttatc ctataattag
     5881 cggattcatc tcacgagtca ccccgtaacg ttcagagagc gtttttagat gtctatttat
     5941 aaaatcccgc ttccactgaa tattctggaa gctgcaaaag aacgtattac ctggacactt
     6001 aataccctac cccgtatctg tgtttctttt tcgggtggta aagattccgg attaatgctg
     6061 catctgaccg ccgaaattgc ccgacaaatg ggcaaaaaaa tctgcgtttt gtttatcgac
     6121 tgggaagcgc agttctcatg cactattaac tatgttcagt cattacgtga gttttacgcc
     6181 gatgtcatcg aagagtttta ctgggttgcg ctcccgctta cgacgcaaaa ttccctttct
     6241 caataccaac ccgaatggca gtgctgggaa cctgatgtcg aatgggtacg tcagcccccg
     6301 caagatgcga taaccgatcc tgactttttc tccttttacc agcctggcat gaccttcgaa
     6361 caatttgtgc gtgagtttgc cgaatggttt tcgcaaaaac gtccggcggc gatgatgatc
     6421 ggcatccgtg cggatgagtc ctacaaccgt tttgtcgcca tcgccagttt aaataaacaa
     6481 cgttttgccg atgataagcc ctggactact gccgcaccag gcggtcatag ctggtacatt
     6541 taccccatct acgactggaa agtcgctgat atctggacct ggtatgctaa ccatcaacag
     6601 ctctgtaacc cactgtataa tataatgtat caggcaggcg ttcctctgcg tcatatgcga
     6661 atttgcgaac cttttggccc ggagcaacgg caaggattat ggctctatca cgttatcgaa
     6721 ccggatcgct gggctgctat gtgcgcacga gtcagcgggg taaaaagtgg cggcatttac
     6781 gccggacatg ataatcattt ttatggtcat cggaaaatcc tcaagccaga acatttagac
     6841 tggcaagaat atgcattatt gctgctcaat agcatgccgg aaaaaacagc tgagcattac
     6901 cgcaataaaa tcgccattta tttgcactgg tatcagaaaa aaggcatcga agttccacaa
     6961 acccagcaag gggacattgg cgcgaaagat atcccctcct ggcggcgaat atgcaaagtt
     7021 ttactcaata acgattactg gtgccgggca ttatcattta gtcctacgaa agcgaagaac
     7081 tatcagcgtt ataacgaacg gataaaagga aaacgtcagg aatgggggat actatgcaac
     7141 aacgattaac gcaggattta acccaatttc tcgccagctt gccggaagat gatcgcatca
     7201 aagcgattaa tgaaatccgc atggcgatcc atcaggttag cccctttcgt gaagagccag
     7261 tggattgcgt tctgtgggtt aaaaacagcc aacttatgcc taatgattac aacccaaaca
     7321 acgtggcacc acccgaaaaa aaactgctga aaaaatccat tgaaatagat ggttttaccc
     7381 aacccattgt ggtcacgcat acggataaaa atgccctgga aatcgtggat ggttttcacc
     7441 gccatgaaat tggtaaaggc tcatcatcgt taaaattacg cttaaaaggt tatttgccgg
     7501 tgacctgtct ggaaggtacg cgtaatcaac gcatcgccgc aactattcgt cataatcgcg
     7561 ctcgtggacg tcaccaaatc actgccatgt cagaaatcgt ccgagaactc agccagttgg
     7621 gatgggacga taataaaatt ggcaaagagc tgggaatgga cagcgacgaa gtgttgcgcc
     7681 tgaagcaaat taacggcctg caagagttgt ttgccgaccg tcaattttcc cgcgcctgga
     7741 cggttaaata actaaagctg gcgcaggcgt tctgctgctg ccagcaacgt cgattccttc
     7801 ttggcaaaac agagacgaat cagtttatgt gggaagggat cggcgcaaaa caccgacagc
     7861 ggaatcgccg ctacgccgtg ctcccgcgtc agccactggc aaaactcaac atcatccagc
     7921 gtagatacgg cgctgtaatc caccagcaaa aagtatgtac cttcgcacgg taaaatctcc
     7981 agccgacttt catttaaggc attcaccaga atatcgcgct tctggcgata aaagtccggt
     8041 aacgcaagat aatgctcagg ttctgcacgt agcatatcgg caagcgccaa ctgtgccggg
     8101 gtattcaccg aaaaggtcag atactgatgc actttgcgga tctcggcgct gatgggcgca
     8161 ggtgcaacgc aataccccac tttccagccg gtcatatgat aggtcttgcc aaacgatgac
     8221 accgccaccg ctcgctcaca caattgcgga tgcgccagca cgctggcatg gccctgttgt
     8281 gaaaaattga tgtgctcgta gacttcatcg ctaatgacaa aaatctcgtg cccggcaatc
     8341 gcctgccaca gagcggagaa atcagcctgc tgccagacag ttgcactggg gttatgcggg
     8401 gtgttgagga tcaccagtcg ggtgcgctcg cttaacaatg cggcaaattc ctgccagtcc
     8461 acgcgaaaat gcggtggttg cagtgcaata cgtttcacta ttccccccga aagcgcgatg
     8521 gcgggcgcgt aactgtcata gctgggatca aagcaaatca cttcatcgcc attgcgaacc
     8581 aatgcagtaa tcgctgcgta tagcgcttcc gtcgcccctg ctgttacggt gatatcgctg
     8641 tcggcatctg gttgatagcc atacagccgt tccgttttct gagcaatcgc ctcgcgtaat
     8701 gcctgcacgc cggtcatggg tgcgtattgg tttgccccct gggcaacgtg gtacgccagc
     8761 cgttcctgca aatagcgcgg accatcaaaa tcaggaaagc cttgcgacag gttaatcgcc
     8821 tggtgttgct gcgccagtgc actcatctgg gtgaaaatag tggtgccaag ttgtggaagt
     8881 ttgctttgtg gaatcagagg gttatttgtc atttttattg tgcctgatga agttgcgtgt
     8941 tgaaggcact ataacacgat gctagtattt ggcaatcaag acgtttagat gtctaaataa
     9001 aacaataagg acaacacaac atgcctcaca atcctatccg cgtggtcgtc ggcccggcta
     9061 actacttttc acatcccgga agtttcaatc acctgcacga ttttttcact gatgaacaac
     9121 tttctcgcgc ggcgtggatc tacggcgaac gcgccattgc tgcggcgcaa acaaaacttc
     9181 cgccagcgtt tgaactgcca ggggcaaagc atattttgtt tcgtggtcat tgcagcgaaa
     9241 gcgatgtaca acaactggcg gctgagtccg gtgacgatcg cagcgtggtg attggcgtcg
     9301 gcggcggcgc actgctcgac accgcgaaag ccctcgcccg ccgtctcggt ctgccgtttg
     9361 ttgccgttcc gacgatcgcc gctacctgcg cagcctggac accgctctct gtctggtaca
     9421 acgatgccgg acaagcgctg cattatgaga ttttcgacga cgccaatttt atggtgctgg
     9481 tggaaccgga gattatcctc aacgcgccgc aagaatatct gctggcgggg atcggtgaca
     9541 cgctggcgaa atggtatgaa gcggtggtgc tggccccgca accagaaacg ttgccgttaa
     9601 ccgtgcggct ggggattaat aacgcgcagg ccattcgcga cgtcttgtta aacagtagcg
     9661 aacaggcgct tgccgatcag caaaatcacc agttaacgca atcattttgc gatgtggtgg
     9721 atgccattat tgctggtggt gggatggttg gtggtctggg cgatcgtttt acgcgtgtgg
     9781 cggcagctca tgccgtgcat aacggtctga ccgtgctgcc gcaaaccgag aagtttctcc
     9841 acggcaccaa agtcgcctac ggaattctgg tgcaaagcgc cttgctgggt caggatgatg
     9901 tgctggcgca attaactgga gcgtatcagc gttttcatct gccgactacg ctggcggagc
     9961 tggaagtgga tatcaataat caggcggaga tcgacaaagt gattgcccac accttacgtc
    10021 cggtggaatc cattcattac ctgccggtca cgctgacacc agatacgttg cgtgcagcgt
    10081 tcgaaaaagt ggaatcgttt aaagcctgac gtacatattt agcagcagcg cgcgccgcct
    10141 tttccaccgt agcgcgcgtc ctgccgctca cggaaaaact cttcgtaggt catcggcgtt
    10201 tgatcgggat ggttaacccg catatgttcg acatagttgt cgtaatcagg cataccaatc
    10261 atcagtttcg ccgcctgacc taaatatttt ccggcttttg ccagtgaatc aaacatctaa
    10321 acctccggta atacaaatgc cctcctgaat taagagggct taattgctat caacacgctc
    10381 gggatgagag taaggggaac gctcagcaca ttccccctca ctctgacttt agtgcgcgcc
    10441 tttcgcctgc gccacgatct cctcgacatt ttccggcatc ggctcatacg gcgtttcttt
    10501 cgccgttggc ttcggctctt tcaatgccgc cagtgccgtc ttaatcgaga acagtgccag
    10561 aaccaccacg accaccataa agaagatggt taacccggca tccagacggt tgttgaacac
    10621 cagttgcgcc agctgtgact cggtatactg cgacggaata ttgccgctgt cgatcattgc
    10681 ctggaacttg ttagcaatcg ccaggaagcc gacttttgca tccaggctaa acgctttctg
    10741 ccagcctgcg gtcagggtac aaatcagcag ccaggccgtt ggtaccagcg ccacccaggc
    10801 gtaacgttga cgtttcattt tgaacagcac cacagcacag agcatcagcg ccatccctgc
    10861 cagcatctgg ttggcaatac caaacagcgg ccacagagtg ttaataccgc ccagcggatc
    10921 gaccacgccc tgatggagga agtagcccca cgccagcacg cacagcgccg ttgccagcag
    10981 gttagcaggc agtgaatcgg tccgtttcag gcccggagac accacgccca gcagatcctg
    11041 caacataaag cgcgcagcac gcgtacctgc atccaccgcc gtcagaataa acagtgcttc
    11101 aaacagaatg gcgaagtgat accagaacgc cacatccatc atgccgccca gcgcaccgtg
    11161 cagaatgtag gccatcccca ccgccagggt cggcgcaccg cccgcacggg aaatgatcga
    11221 ctgttccccc acttcgctgg caatctgatt tagcgtatcc ggggtaatcg caaagcccca
    11281 gctactcacc acctgcgcgg cagaagcgac tacatccgcc gtccctgccg gagccagcac
    11341 tgccatcggg ctgttcatag cgaagtacac acctggatcg atgatacagg cggaaaccag
    11401 cgccataatc gccacgaagg attccatcaa catcccaccg tagccgataa agcacgcctg
    11461 cccttcgttc gccagcatct
//
LOCUS       DAMZFT010000148        11363 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.148, whole genome shotgun sequence.
ACCESSION   DAMZFT010000148 DAMZFT010000000
VERSION     DAMZFT010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11363)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11363)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11363
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.148"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(37..273)
                     /locus_tag="PN977_004201"
     CDS             complement(37..273)
                     /locus_tag="PN977_004201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313355.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1435 domain-containing protein"
                     /protein_id="HDI6044177.1"
                     /translation="MLQRTLGSGWGVLLPGLLIAGLMYADLSPDQWRIIILMGLVLTP
                     VMLYHKQLRHYVLLPSCLALIAGIMLMIMNLKQG"
     gene            414..1202
                     /gene="fhuF"
                     /locus_tag="PN977_004202"
     CDS             414..1202
                     /gene="fhuF"
                     /locus_tag="PN977_004202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore-iron reductase FhuF"
                     /protein_id="HDI6044178.1"
                     /translation="MAYRSAPLYEDVIWRTHLQPQDAGLAQAVRATIAEHREHLLEFI
                     RLDEPAPLNAMTLAQWSSPNALSSLLAVYSDHIYRNQPLMIRENKPLISLWAQWYIGL
                     MVPPLMLALLTQEKALDVSPEHFHVEFHETGRAACFWVDVCEDKNATPHSPQQRMETL
                     ISQALVPVVQALEATGEINGKLIWSNTGYLINWYLTEMKQLLGEATVESLRHALFFEK
                     MLTNGEDNPLWRTVVLRDGLLVRRTCCQRYRLPDVQQCGDCTLK"
     gene            complement(1241..1918)
                     /gene="bglJ"
                     /locus_tag="PN977_004203"
     CDS             complement(1241..1918)
                     /gene="bglJ"
                     /locus_tag="PN977_004203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418786.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator BglJ"
                     /protein_id="HDI6044179.1"
                     /translation="MEHSRIKKRNVALIEKCVMSSIGIESLFRKFAGNPYKLHTYTSQ
                     ESFQDAMSRISFAAVIFSFSAMRSARREGLSCLTELAIKFPRTRRLVIADDDIEARLI
                     GSLSPSPLDGVLSKASTLEIFHQELFLSLNGVRQATDRLNNQWYINQSRTLSPTEREI
                     LRFMSRGYSMTQIAEQLKRNIKTIRAHKFNVMSKLGVSSDAGLLEAADILLCMRHCEA
                     SNVLHPY"
     gene            complement(1876..2601)
                     /locus_tag="PN977_004204"
     CDS             complement(1876..2601)
                     /locus_tag="PN977_004204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDI6044180.1"
                     /translation="MLPGCCKNGIVISKIPVMQAGLKEVMRTHFPEYEIISSASAEDL
                     TLLQLRRSGLVIADLAGESEDPRSVCEHYYSLISQYREIHWVFMVSRSWYSQAVELLM
                     CPTATLLSDVEPIENLVKTVRSGNTHAERISAMLTSPAMTETHDFSYRSVILTLSERK
                     VLRLLGKGWGINQIASLLKKSNKTISAQKNSAMRRLAIHSNAEMYAWINSAQGARELN
                     LPSVYGDAAEWNTAELRREMSHS"
     gene            3219..3989
                     /locus_tag="PN977_004205"
     CDS             3219..3989
                     /locus_tag="PN977_004205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter ThrE family protein"
                     /protein_id="HDI6044181.1"
                     /translation="MQTEQQRAVTRLCIQCGLFLLQHGAESALVDELSSRLGRALGMD
                     SVESSISSNAIVLTTIKDGQCLTSTRKNHDRGINMHVVTEVQHIVILAEHHLLDYKGV
                     EKRFSQIQPLRYPRWLVALMVGLSCACFCKLNKGGWDGAVITFFASTAAMYNRQLLAQ
                     RHLHPQINFCLTAFAATTISGLLLQLPTFSNTPTIAMAASVLLLVPGFPLINAVADMF
                     KGHINTGLARWAIASLLTLATCVGVVMALTIWGLRGWV"
     gene            3980..4453
                     /locus_tag="PN977_004206"
     CDS             3980..4453
                     /locus_tag="PN977_004206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter"
                     /protein_id="HDI6044182.1"
                     /translation="MGVIEFLLALAQDMILAAIPAVGFAMVFNVPVRALRWCALLGSI
                     GHGSRMILMTSGLNIEWSTFMASMLVGTIGIQWSRWYLAHPKVFTVAAVIPMFPGISA
                     YTAMISAVKISQLGYSEPLMITLLTNFLTASSIVGALSIGLSIPGLWLYRKRPRV"
     gene            4560..5099
                     /gene="dnaT"
                     /locus_tag="PN977_004207"
     CDS             4560..5099
                     /gene="dnaT"
                     /locus_tag="PN977_004207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaT"
                     /protein_id="HDI6044183.1"
                     /translation="MSSRVLTPDVVGIDALVHDHQTVLAKAEGGVVAVFANNAPAFYA
                     VTPARLAELLALEEKLARPGSDVALDDQLYQEPQAAPVAVPMGKFAMYPDWQPDADFI
                     RLAALWGVALREPVTTEELASFIAYWQAEGKVFHHVQWQQKLARSLQIGRASNGGLPK
                     RDVNTVSEPDSQIPPGFRG"
     gene            5102..5839
                     /gene="dnaC"
                     /locus_tag="PN977_004208"
     CDS             5102..5839
                     /gene="dnaC"
                     /locus_tag="PN977_004208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication protein DnaC"
                     /protein_id="HDI6044184.1"
                     /translation="MKNVGDLMQRLQKMMPAHIKPAFKTGEELLAWQKEQGAIRSAAL
                     ERENRAMKMQRTFNRSGIRPLHQNCSFENYRVECEGQMNALSKARQYVEEFDGNIASF
                     IFSGKPGTGKNHLAAAICNELLLRGKSVLIITVADIMSAMKDTFRNSGTSEEQLLSDL
                     SNVDLLVIDEIGVQTESKYEKVIINQIVDRRSSSKRPTGMLTNSNMEEMTKLLGERVM
                     DRMRLGNSLWVIFNWDSYRSRVTGKEY"
     gene            5888..6382
                     /locus_tag="PN977_004209"
     CDS             5888..6382
                     /locus_tag="PN977_004209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2501 domain-containing protein"
                     /protein_id="HDI6044185.1"
                     /translation="MMKTVKHLLCCAIAASALISTGVHAASWKDALSSAASELGNQNS
                     TTQEGGWSLASLTSLLSSGNQALSADNMNNAAGILQYCAKQKLASVTDAENIKNQVLE
                     KLGLNSEEQKEDTNYLDGIQGLLKTKDGQQLNLDNIGTTPLAEKVKTKACDLVLKQGL
                     NFIS"
     gene            6636..8927
                     /gene="opgB"
                     /locus_tag="PN977_004210"
     CDS             6636..8927
                     /gene="opgB"
                     /locus_tag="PN977_004210"
                     /EC_number="2.7.8.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerol--membrane-oligosaccharide
                     glycerophosphotransferase"
                     /protein_id="HDI6044186.1"
                     /translation="MSELLSFALFLASVLIYAWKAGRNTWWFAATLTVLGLFVVLNIT
                     LFASDYFTGDGINDAVLYTLTNSLTGAGVSKYILPGIGIVLGLTAVFGALGWILRRRR
                     HHPHHFGYSLLALLLALGSVDASPAFRQITELVKSQSRDGDPDFAAYYKEPSKTIPDP
                     KLNLVYIYGESLERTYFDNEAFPDLTPELGALKNEGLDFSHTQQLPGTDYTIAGMVAS
                     QCGIPLFAPFEGNASASVSSFFPQNICLGDILKNSGYQNYFVQGANLRFAGKDVFLKS
                     HGFDHLFGSEELKSVVADPHYRNDWGFYDDTVLDEAWKKFEELSRSGQRFSLFTLTVD
                     THHPDGFISRTCNRKKYDFDGKPNQSFSAVSCSQENIATFINKIKASPWFKDTVIVVS
                     SDHLAMNNTAWKYLNKQDRNNLFFVIRGDKPQQETLAVKRNTMDNGATVLDILGGDNY
                     LGLGRSSLSGQSMSEIFLNIKEKTLAWKPDIIRLWKFPKEMKEFTIDQQKNMIAFSGS
                     HFRLPLLLRVSDKRVEPLPESEYSAPLRFQLADFAPRDNFVWVDRCYKMAQLWAPELA
                     LSTNWCVSQGQLGGQQIVQHVDKTTWKGKTAFKDTVIDMARYKGNVDTLKIVDNDIRY
                     KADSFIFNVAGAPEEVKQFSGISRPESWGRWSNAQLGDEVKIEYKHPLPKKFDLVITA
                     KAYGNNASRPIPVRVGNEEQTLVLGNEVTTTTLHFDNPTDADTLVIVPPEPVSTNEGN
                     ILGHSPRKLGIGMVEIKVVEREG"
     gene            complement(9065..10087)
                     /gene="lgoD"
                     /locus_tag="PN977_004211"
     CDS             complement(9065..10087)
                     /gene="lgoD"
                     /locus_tag="PN977_004211"
                     /EC_number="1.1.1.414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-galactonate oxidoreductase"
                     /protein_id="HDI6044187.1"
                     /translation="MSTMNVLICQQPKELVWKQREIPIPGDNEALIKIKSVGICGTDI
                     HAWGGNQPFFSYPRVLGHEICGEIVGLGKNIADLKNGQQVAVIPYVACQQCPACKSGR
                     TNCCEKISVIGVHQDGGFSEYLSVPVANILPADGIDPQAAALIEPFAISAHAVRRAAI
                     APGEQVLVVGAGPIGLGAAAIAKADGAQVVVADTSPARREHVATRLELPVLDPSAEDF
                     DAQLRAQFGGSLAQKVIDATGNQHAMNNTVNLIRHGGTVVFVGLFKGELQFSDPEFHK
                     KETTMMGSRNATPEDFAKVGRLMAEGKITADMMLTHRYPFATLAETYERDVINNRELI
                     KGVITF"
     gene            10226..11140
                     /locus_tag="PN977_004212"
     CDS             10226..11140
                     /locus_tag="PN977_004212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDI6044188.1"
                     /translation="MSRSQNLRHNVINQVIDDMARGHIPSPLPSQSALAEMYNISRTT
                     VRHILSHLRECGVLTQVGNDYVIARKPDHDDGFACTTASMSEQNKVFEQAFFTMINQR
                     QLRPGETFSELQLARAAGVSPVVVREYLLKFGRYNLIQSEKRGQWSMKQFDQSYAEQL
                     FELREMLETHSLQHFLNLPDHDPRWLQAKTMLERHRLLRDNIGNSFRMFSQLDRDFHS
                     LLLSAADNIFFDQSLEIISVIFHFHYQWDESDLKQRNIIAVDEHMTILSALICRSDLD
                     ATLALRNHLNSAKQSMIRSINENTRYAH"
BASE COUNT         2967 a         2882 c         2652 g         2862 t
ORIGIN      
        1 tatctttact tcctttcttg ttgcttccct gttttatcat ccctgcttca aattcattat
       61 catcagcatg atgccagcaa taagcgccag gcacgatggc agcaaaacgt aatgccgcaa
      121 ctgtttgtga tacagcatca ccggcgtcaa tactaatccc atcagaatga taatccgcca
      181 ttgatccggc gataaatccg catacatcag ccctgcaatc agcaatcccg gcagcaacac
      241 tccccagcca ctgcccagcg tacgttgcaa catggtttca tctctttcat tgataatgat
      301 aactaatatc atatgataat ttttatcatt tgcaagccag agaaatccct tgctatcggg
      361 taaacctatc gctaggatta gcaatcatta tcatttagat tactatcgcg attatggcct
      421 atcgttccgc accgctctat gaagatgtta tctggcgaac gcatctccag ccgcaggatg
      481 ctgggcttgc acaagccgta cgggcgacga tcgccgaaca tcgtgaacat ttgctggagt
      541 ttatccgcct ggatgaaccc gctccgctta acgccatgac gctggcgcaa tggtcatcac
      601 cgaatgcgct aagttctcta ctcgcggtct attccgatca tatctatcgc aaccaacctt
      661 tgatgatccg cgagaacaaa ccgctgatct cactatgggc acaatggtat atcggcctga
      721 tggtgccacc attaatgttg gcgctgctga cgcaggaaaa agcattagat gtgtcgccgg
      781 aacatttcca cgttgagttt cacgaaaccg gacgcgccgc ctgtttctgg gtcgatgtgt
      841 gtgaagataa aaacgcaaca ccacattcgc cgcagcagcg aatggaaacg ttaatcagcc
      901 aggcgctggt tccggttgtg caggcattag aagcgacggg agagatcaac ggcaaactta
      961 tctggagtaa taccggttat ttgatcaact ggtatctcac tgagatgaag cagctgcttg
     1021 gcgaggcgac ggttgaatcg ctgcgccatg ccctcttttt tgagaaaatg ctcacgaacg
     1081 gtgaagataa tccactctgg cgtaccgtgg tactgcgcga cggcctgctg gtgcgccgga
     1141 cctgctgcca gcgttatcgc ttaccggatg tgcagcaatg tggtgactgt acgctgaaat
     1201 agaaaaattt gccggggaag taaacccggc attaagcgta ttaatagggg tgcaacacgt
     1261 tacttgcttc gcaatgacgc atacataaca gaatgtctgc ggcctccaac aaccctgcat
     1321 cagaactgac gcccagtttc gacatcacat taaatttatg tgcacggatc gttttgatat
     1381 tgcgtttaag ctgctcggca atttgtgtca ttgagtagcc acgcgacata aagcgcaata
     1441 tttctctctc cgtcgggctt aacgtccggc tttggttaat gtaccactga ttgttcagtc
     1501 gatcggtcgc ctgacgtaca ccatttaatg acaaaaagag ttcctgatga aaaatctcca
     1561 gcgttgacgc tttacttaat acaccgtcca gcggtgatgg cgacaatgaa ccaatcagcc
     1621 gagcttcaat atcatcatcc gcaataacta aacgccgggt acgcggaaac ttaatcgcca
     1681 gttcagtcag gcaggataat ccctctctgc gcgcacttct catggcagaa aaagaaaaaa
     1741 tgaccgccgc aaacgagatc cgcgacatgg catcctgaaa tgactcctga ctggtatagg
     1801 tatggagctt ataagggtta cccgcaaact ttctgaataa actctcaata ccgatactgc
     1861 tcatgacgca tttttctatg agtgcgacat ttctcttctt aattcggctg tgttccattc
     1921 tgcggcatct ccataaacag aaggcaagtt aagttctctt gcaccctgcg cgctatttat
     1981 ccatgcatac atttcagcgt tgctgtgaat tgccagtcga cgcatcgcac tgtttttttg
     2041 ggcgctgata gttttattac ttttcttaag caatgaagct atctggttga tgccccatcc
     2101 tttacctaat agccgcagca ctttgcgctc tgaaagagtg agaatgacgg agcgatagct
     2161 aaaatcatga gtttcagtca ttgccggaga ggtcagcatg gcgctgatac gctctgcgtg
     2221 cgtattgccg gaacgtacgg tcttgaccag attctcaatg ggttcaacat cagacaataa
     2281 cgtcgccgta gggcacataa gcagttctac tgcctgggaa taccaggagc gtgacaccat
     2341 gaaaacccag tgaatttccc gatattgcga gattaaagaa taataatgtt cacaaacaga
     2401 acgtggatct tcactttcac cggctaaatc agcaatgact aatccggaac gacgtaattg
     2461 taataaggta aggtcctccg cagaggcgct ggatattatt tcatattcag ggaagtgagt
     2521 cctcatgacc tcttttaacc ctgcttgcat aacaggtatt ttactgataa caattccatt
     2581 tttgcagcat cctggcaaca tatttcctcc acatccactg tctaaaaatc atgatggttt
     2641 attcaggata ttcattcctt aaatgtttcc agcaaattaa tagattaagc gaataaatta
     2701 tccttagcat tcactttcat atcagcatac tcctgctgca caacaactcc actgcatgtg
     2761 tcagaaggtt agcatcaaat gataacctgg tgaaatagcc tggagacatt taaaatatct
     2821 acattaagtt gttagaaata cttaatgaaa aacagaattc tcattcactt aatttataat
     2881 taactgatat cccttcccgc aggatgcaaa atcacacaat caatatttat tcaataaaat
     2941 ctgatagttt taacttgtta aaattaaaaa acgcgcccag ttatctttta tcctgattcg
     3001 taagggctaa acaataaatt cattatagtg ataatattca tgcgataatt tgtttgcggc
     3061 gagagataat tcgcttttaa caccgagctt ctcccgataa ccaggattaa tcagcgcaat
     3121 tttgatctta acctgccgca aaacaaccgc gcttcatgat acaaatggat tctcattatc
     3181 tgaacaatac gcagcacgtc tatgacaaag ggcgagttat gcaaactgag caacagcgag
     3241 ccgtaacacg gctttgtatc cagtgtggat tatttctttt gcaacatggt gcggaaagcg
     3301 cgttggttga tgagctttcc tcacgactgg gtcgggcgtt gggaatggac agcgtcgaaa
     3361 gttctatctc ttcgaacgcc atagtgctga caactattaa agatgggcaa tgcctgacat
     3421 ctacacgtaa aaatcacgat cgcggcatta atatgcatgt ggtgactgaa gtccagcaca
     3481 ttgtgattct ggcggagcac catctgctgg attacaaagg cgtagagaaa cgatttagcc
     3541 aaattcagcc attacgttac ccaagatggc tggtagcctt aatggttggc ctttcctgcg
     3601 cctgtttctg taaactcaat aaaggtggct gggatggtgc cgtcatcacc ttctttgcca
     3661 gtacggccgc gatgtataac cgccagctac tggcacaacg tcatcttcat ccacagatca
     3721 acttttgcct caccgctttc gccgccacca ccatttccgg attgcttttg caacttccca
     3781 ctttcagcaa tacccccacc attgcgatgg ccgccagcgt tctgctgctg gtgccgggct
     3841 ttccgttgat aaatgccgtc gccgatatgt ttaaaggcca catcaatacc ggactggcac
     3901 gctgggcgat cgccagtctg ctgacactgg ctacctgtgt cggcgtagta atggcactga
     3961 cgatttgggg gctacgcgga tgggtgtgat cgaatttctg ttagcgttgg cgcaggatat
     4021 gatcctcgcc gccattccag cggtcggctt tgcaatggtg ttcaacgttc ccgtgcgggc
     4081 gttacgctgg tgtgcgctgc ttggctcgat aggtcatggt tcccgaatga tcttaatgac
     4141 cagcgggttg aatattgagt ggtcaacttt tatggcttct atgctggtcg gcaccattgg
     4201 tattcaatgg tcgcgctggt atctggcaca tccgaaagtg tttaccgtgg cggctgttat
     4261 ccctatgttc cctggcatat cagcttatac cgcaatgatt tcggcggtaa aaatcagcca
     4321 gctaggttac agcgaaccgt tgatgattac cctgttaacc aactttctta cagcttcatc
     4381 gattgttggt gcgttatcca tcggtctttc cattcctgga ttatggttgt accgcaagcg
     4441 ccctcgcgta taaaattgcc tcgcttaagc acacggatga gagacagcct cctctcctcc
     4501 gtgtgttact ataaaagtta tctcccttct cgttcatcgt tccatatttg agaaacagta
     4561 tgtcttccag agttttgacc ccggacgtcg ttggtattga cgccctggta cacgatcacc
     4621 aaaccgttct ggcaaaagct gaaggcggtg tggttgccgt atttgctaac aatgccccgg
     4681 cgttttatgc tgtcacgcct gcacgcctgg ctgaactgct ggcgctggaa gaaaagctgg
     4741 cgcgtccggg aagcgatgtc gctctggacg atcaactcta tcaggaaccg caagccgctc
     4801 ccgttgctgt acccatgggg aaattcgcca tgtatccgga ctggcaaccc gatgccgact
     4861 ttatccgcct ggcggcgcta tggggcgtgg cgctaagaga gccggtgacc accgaagaac
     4921 tggcctcatt cattgcctac tggcaggcgg aaggtaaagt ctttcaccat gtgcagtggc
     4981 aacaaaaact ggcgcgcagc ctgcaaatcg gccgtgccag caacggcgga ctgccgaaac
     5041 gagatgtgaa tacggtcagt gaacctgaca gccaaattcc accaggattc agagggtaac
     5101 gatgaaaaac gttggcgacc tgatgcaacg cctgcaaaaa atgatgcctg cccatatcaa
     5161 acctgcgttc aaaacgggtg aagaactact ggcctggcag aaagaacaag gagcgatccg
     5221 ctccgccgct ctcgaacgtg aaaatcgggc gatgaaaatg cagcgcacct ttaaccgctc
     5281 cggtattcgt ccactgcatc agaactgctc ctttgagaac tatcgcgttg agtgtgaagg
     5341 gcagatgaat gcgttaagca aagcgcgcca gtatgtcgaa gaatttgacg gcaacatcgc
     5401 cagcttcatc ttttctggca agcccggaac cggcaaaaac catctggcgg cggcaatctg
     5461 caacgaactg ctgctacgcg gtaaatccgt attgatcatc accgtggctg atattatgtc
     5521 ggcgatgaaa gataccttca ggaatagcgg taccagcgaa gaacaactgc ttagcgatct
     5581 gagcaacgtt gatctgctgg tgatcgatga gatcggcgtg cagaccgaat cgaaatacga
     5641 aaaagtgatc atcaaccaga tcgtcgatcg ccgctcttct tccaaacgcc caaccgggat
     5701 gctgaccaac agtaatatgg aagagatgac caagttgctg ggcgaacgcg tgatggaccg
     5761 tatgcgcctg ggtaacagtt tgtgggtgat cttcaactgg gatagctacc gtagccgggt
     5821 aacgggtaaa gagtattaat ccccgtttct ggcggtatac tgcgacacaa ttccaccttg
     5881 agtaatgatg atgaaaactg ttaaacatct tctgtgctgt gccattgccg ccagcgcatt
     5941 aatttccacc ggggtgcatg ctgcgtcctg gaaagatgcg ctctccagcg cagccagcga
     6001 acttggcaac caaaacagca cgacacagga aggcggttgg tcgctcgctt cattaactag
     6061 cttgcttagc agcgggaacc aggccttaag cgcagataac atgaacaatg ccgcaggcat
     6121 actgcaatac tgcgcgaagc agaagttggc ttcggtaacc gatgccgaaa acatcaagaa
     6181 tcaggtactg gaaaaactgg gcctgaacag tgaagagcaa aaagaagaca ccaactatct
     6241 ggatggcatt cagggtttgc tgaaaaccaa agatggtcag caactcaatc tggataatat
     6301 tggaacgact ccgctggcag aaaaggtgaa aaccaaagcc tgcgatctgg tgttaaagca
     6361 ggggctgaac ttcatttcct gaaccgcgaa acgccgcgtt ttagctgtta cccggctgac
     6421 gcggctctct cctgcccttt tccctgctcc tggacggttt tacccctgtt acacgggaag
     6481 cgaccgtaat caaaaaagtc tgtttataaa ctaattccta acaacatcgc atttttttga
     6541 cactagaatt gcagcaatat ggcgacgcca ttacattgtg cttttcaaaa agcttaaacg
     6601 ttggctaaac ggcccggttt agtgaggatc gcccgttgtc agaactactc tctttcgccc
     6661 tttttctcgc ctctgtgctg atttacgcat ggaaagcggg acgtaacacc tggtggtttg
     6721 cagccacgtt aacggtgctg gggctatttg tcgttttaaa tatcaccctg tttgccagcg
     6781 actattttac tggcgatggt attaacgacg cggttctcta taccttaacc aacagcctga
     6841 ccggtgctgg cgtcagcaaa tacattctgc cgggtatcgg cattgtgctg gggctgacag
     6901 cggtgttcgg tgcgctgggc tggatcctgc gccgtcgtcg tcatcatccg caccattttg
     6961 gttacagcct gctggcactc ttactggcgc tgggttcagt ggatgccagc ccggcatttc
     7021 gtcagataac ggaactggtg aaatcccagt cacgcgacgg cgacccggac tttgcggctt
     7081 attataaaga gccgtcgaaa actatccctg acccgaaact caacctggtt tatatctacg
     7141 gcgaaagtct cgagcgaacc tattttgata acgaagcttt cccggatctc acgcctgaac
     7201 tgggcgcgtt gaaaaatgaa ggcctggatt tcagccacac gcagcagctg ccaggaacgg
     7261 attacacgat tgcgggcatg gtggcttctc agtgcggcat accgctgttt gccccctttg
     7321 aaggcaacgc ctccgcctct gtctccagct tcttcccgca gaacatctgt ctgggcgata
     7381 tcctgaaaaa ctcgggttat cagaactatt tcgtgcaggg cgcgaatctg cgttttgccg
     7441 gtaaagatgt gttcctgaaa tcgcacggct tcgaccactt attcggctca gaagagctga
     7501 aaagcgtggt ggccgacccg cactatcgca acgactgggg attctacgac gataccgttc
     7561 tcgatgaagc gtggaaaaag tttgaagagc tttcccgctc aggtcagcga ttctcactgt
     7621 ttaccctgac agtcgatacc catcacccgg atggttttat ctcgcgtacc tgtaaccgca
     7681 aaaaatatga ttttgacggt aagccgaatc agtcattcag cgcggtaagt tgcagtcagg
     7741 agaatatcgc gacgtttatc aacaaaatca aagcgtcacc gtggtttaaa gatactgtta
     7801 tcgtcgtctc ttctgaccat ttagcgatga acaacacggc gtggaaatac cttaataaac
     7861 aggatcgcaa taacctgttt tttgtcatcc gtggcgacaa gccgcagcaa gagacgctgg
     7921 cggtaaagcg taacacgatg gataacggcg cgacggtgct ggacattctc ggtggcgata
     7981 actatctcgg acttggtcgt agcagtttat ccgggcagtc gatgtcggaa atcttcctca
     8041 atatcaaaga gaaaacattg gcgtggaagc cggatatcat ccgcctgtgg aaattcccta
     8101 aagagatgaa agagttcacc atcgaccagc agaaaaacat gattgccttc tcgggtagcc
     8161 atttccgttt gccgctgctg ttgcgggttt cagacaaacg cgtggaaccg ctgccggaaa
     8221 gtgaatactc agcaccgctg cgtttccagc tggccgattt cgctccacgc gacaatttcg
     8281 tctgggttga ccgttgctac aagatggcac aactttgggc tccggaactg gcactctcca
     8341 ccaactggtg tgtctcgcaa gggcagcttg gcggtcagca aattgtccag catgttgaca
     8401 aaacaacatg gaagggcaaa acggcattta aagatacggt catcgacatg gcgcgttaca
     8461 aaggcaatgt cgatacgctg aagattgttg ataacgatat tcgctacaaa gccgacagtt
     8521 tcatctttaa cgtcgccggt gcgccggaag aggtgaaaca gtttagcggg atttctcgtc
     8581 cggagtcgtg gggccgctgg tccaacgctc agctgggcga tgaagtaaaa atcgagtata
     8641 agcatccgct gccgaagaaa tttgacctgg tgattaccgc caaagcatac ggcaataacg
     8701 ccagccgtcc tattccggta cgcgtaggca atgaagaaca aacccttgta ctgggcaatg
     8761 aagtgaccac caccacgctg catttcgata acccaaccga tgccgacacg ctggtaattg
     8821 tgccgccgga acctgtctca accaacgaag ggaatatcct cggacactcg ccgcgtaagc
     8881 tcgggatcgg catggtggag attaaagtgg tagaacgtga agggtaattc ctgactgatt
     8941 tgtaggtcgc ataaggtgcg gcctacacgc cttctccggc ttacaacaca ctattaagaa
     9001 acaaaaaaaa cagcgcccaa cacttccccg gacgctgcat ggcaacttta ataatctcgc
     9061 cttatcagaa agtaattaca cccttaatca actcacgatt gttaatcaca tcacgttcgt
     9121 aggtttctgc cagcgtggcg aacgggtagc gatgggttaa catcatgtcg gcagtgattt
     9181 tcccttccgc catcaatcga ccgactttag cgaaatcttc cggcgtggcg ttgcggctgc
     9241 ccatcatcgt cgtttctttt ttatggaatt ccggatcgga gaactgcaac tcacctttaa
     9301 acagaccgac aaataccacc gtgccgccgt gacgaatcag atttacggtg ttattcatcg
     9361 catgttgatt acctgtcgcg tcgatcactt tctgcgccag cgaaccacca aactgcgccc
     9421 gcagctgcgc gtcaaaatct tcggctgacg ggtccagtac aggtaattcc agacgcgttg
     9481 ccacatgttc acggcgcgcc ggactggtat ccgccactac cacctgtgcg ccatcggctt
     9541 tagcgattgc cgccgcgccc agaccgattg gccccgcccc gaccaccagc acctgctcgc
     9601 cgggagcaat ggctgcacga cgcaccgcat gtgcgctaat agcgaaaggt tcaatcaatg
     9661 ctgccgcctg cgggtcaata ccgtctgcgg gcaaaatgtt cgccaccggc acgctcaaat
     9721 actcactaaa accaccatcc tgatgcacac caataactga aattttttca cagcaattgg
     9781 tacgaccgct tttacatgca gggcattgct gacaggcaac ataagggatc acagcaactt
     9841 gctgaccatt tttaagatca gcaatatttt tacccagccc aacaatctcc ccacatattt
     9901 catggcctaa aacacgtgga taactaaaaa atggttgatt tccaccccag gcatgaatat
     9961 cggtaccgca aatcccgaca gacttaattt ttattaatgc ttcattgtca cccggaatag
    10021 gtatctcgcg ttgtttccag actaattctt tcggctgctg gcaaattaaa acattcatcg
    10081 tagacatgat tcggctctcc attttttgtt gccttagata tcaatgaaaa tgaaaaatgg
    10141 cagcggaacg gaaaatctgt tttgtgaaaa cacacgcata aaaatgtttt aaatcgggtt
    10201 ttaatggact cacaaggagg aaaatatgag tcgctcacaa aatttacgcc acaatgtgat
    10261 taaccaggtc attgatgata tggcgcgggg tcatatccct tccccacttc catcgcaaag
    10321 tgcgctggcg gaaatgtaca acatcagccg caccacagtg cgtcatattc tcagccactt
    10381 acgcgaatgc ggcgtcttga cgcaggttgg aaacgattat gttattgcgc gtaaacctga
    10441 ccacgatgac ggtttcgcct gtactaccgc ttcaatgagt gaacaaaaca aagtgtttga
    10501 gcaggctttt ttcacgatga taaaccagcg tcagttacgc cctggggaaa ctttttctga
    10561 actgcaactg gcgcgggccg cgggtgtcag cccggtggtt gtaagagaat atcttttaaa
    10621 attcggccgt tataatctta ttcaaagcga aaagcgcggc cagtggagca tgaagcaatt
    10681 cgatcaatcc tacgccgagc agctgtttga gctacgcgaa atgctcgaaa cacattcact
    10741 tcagcacttt ctcaacctgc ctgatcacga tccccgctgg ctgcaggcga aaaccatgct
    10801 cgaacgtcat cggctactgc gtgacaacat cggcaatagc tttcgcatgt tctcgcaact
    10861 tgacagagat tttcactcct tactgctttc agctgccgac aatatctttt ttgatcaatc
    10921 gcttgagatc atctcagtca tctttcattt tcactatcaa tgggacgaaa gcgatcttaa
    10981 acagcgcaat atcattgccg tcgatgagca tatgaccatt ctcagtgctc tgatttgccg
    11041 cagtgacctg gatgccaccc ttgcgctccg taaccatttg aattcagcta aacaatcaat
    11101 gattcgatcc attaatgaga acacgcgtta tgctcattaa gtacggatta aaaacagcaa
    11161 actattagcg ggtgcacggt tttatcaaaa gataaatacc gggaaaatcc cgcttaccta
    11221 tgctcacaat cagtcgacta aaaatgtttt tgtgagcggt agtaaagtcc taaaacttta
    11281 acctgccgtt aatgtcaacc ttgcgtaatg cacaattacg taaacaatta aaattataaa
    11341 tacaggagtc cggcgtggaa aaa
//
LOCUS       DAMZFT010000149        11183 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.149, whole genome shotgun sequence.
ACCESSION   DAMZFT010000149 DAMZFT010000000
VERSION     DAMZFT010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11183)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11183)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11183
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.149"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..84
                     /locus_tag="PN977_004213"
     CDS             <1..84
                     /locus_tag="PN977_004213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SufE family protein"
                     /protein_id="HDI6044189.1"
                     /translation="TQHLTPSRSQGLEAMIRAIRAKAAALS"
     gene            233..1237
                     /gene="ldtE"
                     /locus_tag="PN977_004214"
     CDS             233..1237
                     /gene="ldtE"
                     /locus_tag="PN977_004214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtE"
                     /protein_id="HDI6044190.1"
                     /translation="MKRASLLTLTLIGAFSAIQAAWAVDYPLPPTGSRLVGQNQTYTV
                     QEGDKNLQAIARRFDTAAMLILEANNTIAPVPKPGTTITIPSQLLLPDAPRQGIIVNL
                     AELRLYYYPPGENIVQVYPIGIGLQGLETPVMETRVGQKIPNPTWTPTAGIRQRSLER
                     GIKLPPVVPAGPNNPLGRYALRLAHGNGEYLIHGTSAPDSVGLRVSSGCIRMNAPDIK
                     ALFSSVRTGTPVKVINEPVKYSVEPNGMRYVEVHRPLSAEEQQNVQTMPYTLPAGFTQ
                     FKDNKAVDQKLVDKALYRRAGYPVAVSSGATPAASNTPSVESAQNGEPEQGNMLRATQ
                     "
     gene            complement(1301..1537)
                     /gene="lpp"
                     /locus_tag="PN977_004215"
     CDS             complement(1301..1537)
                     /gene="lpp"
                     /locus_tag="PN977_004215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein lipoprotein Lpp"
                     /protein_id="HDI6044191.1"
                     /translation="MKATKLVLGAVILGSTLLAGCSSNAKIDQLSSDVQTLNAKVDQL
                     SNDVNAMRSDVQAAKDDAARANQRLDNMATKYRK"
     gene            complement(1848..3260)
                     /gene="pykF"
                     /locus_tag="PN977_004216"
     CDS             complement(1848..3260)
                     /gene="pykF"
                     /locus_tag="PN977_004216"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010346010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase PykF"
                     /protein_id="HDI6044192.1"
                     /translation="MKKTKIVCTIGPKTESEEMLAKMLDAGMNVMRLNFSHGDYAEHG
                     QRIQNLRNVMSKTGKTAAILLDTKGPEIRTMKLEGGNDVSLKAGQTFTFTTDKSVIGN
                     SEMVAVTYEGFTTDLSVGNTVLVDDGLIGMEVTAIEGNKVICKVLNNGDLGENKGVNL
                     PGVSIALPALAEKDKQDLIFGCEQGVDFVAASFIRKRSDVIEIREHLKAHGGENIHII
                     SKIENQEGLNNFDEILEASDGIMVARGDLGVEIPVEEVIFAQKMMIEKCIRARKVVIT
                     ATQMLDSMIKNPRPTRAEAGDVANAILDGTDAVMLSGESAKGKYPLEAVSIMATICER
                     TDRVMNSRLEFNNDNRKLRITEAVCRGAVETAEKLDAPLIVVATQGGKSARAVRKYFP
                     DATILALTTNEKTAHQLVLSKGVVPQLVKEITSTDDFYRLGKELALQSGLAHKGDVVV
                     MVSGALVPSGTTNTASVHVL"
     gene            3817..4026
                     /gene="fumD"
                     /locus_tag="PN977_004217"
     CDS             3817..4026
                     /gene="fumD"
                     /locus_tag="PN977_004217"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase FumD"
                     /protein_id="HDI6044193.1"
                     /translation="MGNRTKEDELYREMCRVVGKVVLEMRDLGQEPKHIVIAGVLRTA
                     LANKRIQRSELEKQAMETVINALVK"
     gene            4482..5108
                     /locus_tag="PN977_004218"
     CDS             4482..5108
                     /locus_tag="PN977_004218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein"
                     /protein_id="HDI6044194.1"
                     /translation="MNPVDRPLLDIGLTRLEFLRISGKGLAGLTIAPALLSLLGCKQE
                     DIDSGTVGLINTPKGVLVTQRARCTGCHRCEISCTNFNDGSVGTFFSRIKIHRNYFFG
                     DNGVGSGGGLYGDLNYTADTCRQCKEPQCMNVCPIGAITWQQKEGCITVDHKRCIGCS
                     ACTTACPWMMATVNTESKKSSKCVLCGECANACPTGALKIIEWKDITV"
     gene            5129..7231
                     /locus_tag="PN977_004219"
     CDS             5129..7231
                     /locus_tag="PN977_004219"
                     /EC_number="1.2.7.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase"
                     /protein_id="HDI6044195.1"
                     /translation="MANGWTGNILRVNLTTGNITLEDSSKFKSFVGGMGFGYKIMYDE
                     VPPGTKPFDEANKLVFATGPLTGSGAPCSSRVNITSLSTFTKGNLVVDAHMGGFFAAQ
                     MKFAGYDVIIIEGKAKSPVWLKIKDDKVSLEKADFLWGKGTRATTEEICRLTSPETCV
                     AAIGQAGENLVPLSGMLNSRNHSGGAGTGAIMGSKNLKAIAVEGTKGVNIADRQEMKR
                     LNDYMMTELIGANNNHVVPSTPQSWAEYSDPKSRWTARKGLFWGAAEGGPIETGEIPP
                     GNQNTVGFRTYKSVFDLGPAAEKYTVKMSGCHSCPIRCMTQMNIPRVKEFGVPGTGGN
                     TCVANFVHTTIFPNGPKDFEDKDDGRVIGNLVGLNLFDDYGLWCNYGQLHRDFTYCYS
                     KGVFKRVLPAEEYAEIRWDQLEAGDVNFIKDFYYRLAHRVGELSHLADGSYAIAERWN
                     LGEEYWGYAKNKLWSPFGYPVHHANEASAQVGSIVNCMFNRDCMTHTHINFIGSGLPL
                     KLQREVAKELFGSEDAYDETKNYTPINDAKIKYAKWSLLRVCLHNAVTLCNWVWPMTV
                     SPLKSRNYRGDLAFEAKFFKAITGEDMTQEKLDLAAERIFTLHRAYTVKLMQTKDMRN
                     EHDLICSWVFDKDPQIPVFTEGTDKMDRDDMHASLTMFYKEMGWDPQLGCPTRETLQR
                     LGLEDIAVDLAAHNLLPA"
     gene            7244..7882
                     /locus_tag="PN977_004220"
     CDS             7244..7882
                     /locus_tag="PN977_004220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707570.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdhW family putative oxidoreductase system
                     protein"
                     /protein_id="HDI6044196.1"
                     /translation="MNHQDELPLAEVSEIDEAKRQWLQGMRHPVDTVTEPEPAEILAE
                     FIRQHSAAGQLVARTVFLSPPYSVAEEELPVLLENIKQNGDYADIACMTGSQDDYYYS
                     TQAMSENYAAMSLQVVEQDICRAIAHAVRFECQTYPRPYKVAMLMQAPYYFQEAQIEA
                     AIAAMDVAPEYADIRQVESSTAVLYLFSERFMTYGKAYGLCEWFEVEQFQNP"
     gene            7946..8614
                     /locus_tag="PN977_004221"
     CDS             7946..8614
                     /locus_tag="PN977_004221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416186.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HDI6044197.1"
                     /translation="MSFTRRKFVLGMGTVIFFTGSASSLLANTRQEKKVRYAMIHDES
                     RCNGCNICARACRKTNHVPAQGSRLSIAHIPVTDNDNETQYHFFRQSCQHCEDAPCID
                     VCPTAASWRDEQGIVRVEKSQCIGCSYCIGACPYQVRYLNPVTKVADKCDFCAESRLA
                     KGFPPICVSACPEHALIFGREDSPEIQAWLQENKYYQYQLPGAGKPHLYRRFGQHLIK
                     KENV"
     gene            8611..9396
                     /locus_tag="PN977_004222"
     CDS             8611..9396
                     /locus_tag="PN977_004222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate reductase cytochrome B subunit"
                     /protein_id="HDI6044198.1"
                     /translation="MNPSQHAEQFQSQLANYVPQFTPQFWPVWLIIAGLLLVGMWLVL
                     GLHALLRARGVKKSATDHGEKVYLYSKAVRLWHWSNALLFVLLLASGLINHFALVGAT
                     AVKSLVAVHEVCGFLLLACWLGFVLINAVGGNGHHYRIRRQGWLERAAKQTRFYLFGI
                     MQGEEHPFPATTQSKFNPLQQVAYIGVMYGLLPLLLLTGLLCLYPQAVGDVFPGVRYW
                     LLQAHFALAFISLFFIFGHLYLCTTGRTPHETFKSMVDGYHRH"
     gene            9400..10212
                     /locus_tag="PN977_004223"
     CDS             9400..10212
                     /locus_tag="PN977_004223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6044199.1"
                     /translation="MIITRADLREWRIGAVMYRWFLRHFPRGGSYADIHHALIEEGYT
                     DWAESLVEYAWKKWLADENFAHQEVSSMQKLATDPGERAFCSQFARSDDHARIGCCED
                     NARIATAGYAAQIASMGYSVRIGSVGFNSHIGSSGERARVAVTGNSSRISSAGDSSRI
                     ANTGMRVRVCTLGERCHVASNGDLVQIASFGANARIANSGDNVHIIASGENSTVVSTG
                     VVDSIILGPGGSAALAYHDGERVRFAVAIEGENNIRAGVRYRLNEQHQFVEC"
     gene            complement(10224..11183)
                     /locus_tag="PN977_004224"
                     /pseudo
     CDS             complement(10224..11183)
                     /locus_tag="PN977_004224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310402.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-NAD(P)-binding protein"
BASE COUNT         2881 a         2501 c         2866 g         2935 t
ORIGIN      
        1 acccaacatc tcaccccctc tcgttcacaa ggtctggaag cgatgattcg cgcaattcgc
       61 gccaaagccg ctgcacttag ctaaactaaa gggacagctt tcatccggtt ggctcacatt
      121 gccggatggt caatgtttac actgacctgc acaggagtgt ttaggtttca ggtgaacata
      181 agcgccatcc gacgtttcag cgtgagtctc cggcaaatac aggaggttta caatgaaacg
      241 cgcgtctttg cttacactca cgcttatcgg cgcttttagc gccatccagg ctgcctgggc
      301 ggttgattat ccgctaccac caaccggaag ccgactggtt gggcaaaatc aaacgtatac
      361 ggtgcaagaa ggggataaaa accttcaggc catcgcccga cgttttgata ctgcggcaat
      421 gttgatcctt gaagccaata acactatcgc cccggtgcca aaacctggta cgacgataac
      481 tattccttcg caactgttat tacctgatgc gccgcgtcag gggattatcg ttaacctggc
      541 agagctgcgc ctttattatt atccgccggg agaaaatatc gtgcaggtct atccgatagg
      601 tattggtttg caggggctgg aaacgccagt gatggaaacg cgtgtggggc agaaaatccc
      661 taacccaacc tggacgccta cggcgggcat tcgtcagcgt tcgttggagc gtggcattaa
      721 attaccgcca gtcgttcctg ccgggccaaa taacccacta ggacgttacg cactgcgcct
      781 cgcgcatggt aatggcgaat acctcattca tggcaccagt gcgccggaca gcgtcggttt
      841 gcgcgtcagt tcgggctgta ttcgcatgaa tgcgccggat attaaagcct tattctccag
      901 cgtgcggacg ggaacgccgg tgaaagtgat caacgaaccg gtgaaatatt ccgtggaacc
      961 gaacgggatg cgttatgttg aagtacatcg accactatca gcagaagaac agcagaacgt
     1021 tcagacaatg ccatacacgc tgccagcagg ctttacgcaa tttaaagaca ataaggctgt
     1081 agatcagaag ttagtcgata aagcgttgta tcgtcgggca gggtatccgg ttgcggtgag
     1141 cagtggagca actcccgcag ccagcaatac gccttcagta gagtcagcgc agaatggtga
     1201 accagagcaa gggaatatgt tacgcgcgac gcagtagcgg taaatggcag gcaaaaaaaa
     1261 tggcgcacaa tgtgcgccat ttttcacttc acaggtacta ttacttgcgg tatttagtag
     1321 ccatgttgtc cagacgctgg ttagcacgag ctgcgtcatc tttagcagcc tgaacgtcgg
     1381 aacgcattgc gttcacgtcg ttgctcagct ggtcaacttt agcgttcaga gtctgaacgt
     1441 cagaagacag ctgatcgatt ttagcgttgc tggagcaacc tgccagcaga gtagaaccca
     1501 ggattaccgc gcccagtacc agtttagtag ctttcattat taataccctc tagattgagt
     1561 taatctccat gtagcgttac aagtattaca caaagttttt tatgttgaga atattttttt
     1621 gatgggaagg cacttatttt tgatcgttcg ctcaaagaag catcgaacgc tgggttttag
     1681 ataaaaaaat tgagagaaaa caggatgctt ccatcggatt catcttagat aaagtgagat
     1741 tatatagtga aatccgattt gattttttat tgcttctggt tatcgattaa ataaaaaaag
     1801 cgcccatcag ggcgcttcga tatacaaatt aattcacaaa agcaatatta caggacgtga
     1861 acagatgcgg tgttagtagt gccgctcggt accagtgcac cagaaaccat aactacaacg
     1921 tcacctttgt gtgccagacc gctctgcaga gccagttctt tacccagacg gtagaaatca
     1981 tcagtagaag tgatctcttt aaccagctgc ggcacaacgc ctttgctcag taccaactga
     2041 tgagccgttt tttcgttggt ggtcagtgcc aggatggtgg catccgggaa gtatttacgt
     2101 actgcgcgag cagatttacc gccctgggta gcaaccacga tcagcggagc atccagtttt
     2161 tcagcagttt caacggcacc acggcatacc gcttcggtaa tgcgcagttt acggttgtca
     2221 ttgttgaact cgagacggct gttcatcacg cggtcggtac gttcgcagat ggtcgccatg
     2281 atagaaaccg cttccagcgg gtatttacct tttgcggatt caccagacag catcactgcg
     2341 tcagtaccgt cgaggatggc gtttgcaacg tcaccggctt ctgcgcgagt cgggcgtggg
     2401 tttttgatca tggaatccag catctgggtc gcagtgataa cgactttacg tgcacggata
     2461 catttttcga tcatcatctt ctgggcgaag ataacttctt ctaccgggat ttctacaccc
     2521 aggtcgccac gcgcaaccat gatgccgtca gaggcttcga ggatttcgtc gaagttgttg
     2581 aggccttcct ggttttcgat tttggagatg atgtggatgt tttcgccgcc gtgcgctttc
     2641 aggtgctcac ggatttcgat aacgtcagaa cgcttacgaa taaaggaagc agcgacaaag
     2701 tctacgcctt gttcgcaacc aaagatcagg tcctgtttgt ctttttcagc cagtgctggc
     2761 agggcaatgg aaacgccagg caggttcaca cctttgtttt cacccaggtc gccgttgttc
     2821 agcactttac agataacttt gttaccttca atggcagtaa cttccatacc gatcagacca
     2881 tcgtcaacca gtacggtgtt gccaacagac aggtcagttg tgaaaccttc atacgttacc
     2941 gcaaccattt cgctgttgcc gataacagat ttatcagtag tgaaagtaaa ggtctgacca
     3001 gctttcagag aaacgtcgtt accgccttcc agtttcatgg tgcggatttc cggacctttg
     3061 gtatcaagca ggatagcggc ggttttacca gttttgctca tcacgttgcg cagattctga
     3121 atgcgctgac cgtgttctgc atagtcacca tgagagaagt tcagacgcat aacgttcatg
     3181 ccagcgtcca gcattttagc taacatctct tcagattcgg ttttcggtcc gatggtgcaa
     3241 acaattttgg tctttttcat gacagtctta gtctttaagt tgagaaggat gggagaaact
     3301 tgctttctgg gcgcactgcc ggaaagcgaa tccggtatta cgaaagtggt ggtgcctcgc
     3361 tctaaggata ggtgacatcg aaaaagcgtg cagaggaatg tgtgcttgtg tctcagccca
     3421 acgtgagtgc ctgtttttga gtcgttgagt tctacagtcc aaagtgctga aaccattcaa
     3481 gagtcaattg gcgcgcatta tacgctgaaa ttaatccaaa aggaaaacaa aaacagcgtt
     3541 tcaaaaaata tacaacttgt tagacaataa attgttatca aagatttaac gttccaggga
     3601 aaaggttacg caaaaacttc gctgtttgcg caacgtctgg tgccggggtt gtcactgtga
     3661 agataagtga aagttcatcc ggtgtataaa tacatcatcg ctcaattgct acagttaaaa
     3721 taatttatcg cgttatttgt ctgattttgt ttgatttttg tctaattggc tagattgata
     3781 tatgctgata gttggcatga taaataggta taaagaatgg gaaatcggac aaaagaggat
     3841 gaactgtacc gagaaatgtg cagagttgtc ggtaaagttg tgctggaaat gcgcgatctg
     3901 gggcaggaac caaagcatat tgttatcgca ggcgtattac gtacagcatt agccaataaa
     3961 cgcattcagc gcagtgagct ggaaaagcag gcgatggaaa ccgtcattaa cgcactggta
     4021 aaatgaccat tcaggatgag gcgataagct tcatcctgat attcttgctt cagttgcggt
     4081 cgagttaatc attctaaata acaactgctg aaattatacg aatttcgata tgtgttttaa
     4141 tttataacga taatattatt tttgttttct gcaatgaccg tcacaaataa ttgtcgctta
     4201 agttaaattt atttcaaatg ataataaata tcagttcact tataatttct tgctttgtga
     4261 ttttaattga taacgatcaa tgttaagggc tcagaataat cacattattt tttctcctaa
     4321 ccataaagga ttagtttatc ggcgttaaat cttttctcat acactatcag gttagggaaa
     4381 atatctggtg ttgaggacag agtcagcagc gtattagttg aatgcttaat atcaatctgc
     4441 tgaattgtca gcactgtgtt tgcgaagagg atgcgaaata aatgaacccg gttgatcgtc
     4501 cactattaga tattggctta acgcgattag aattcttgcg tatatcagga aaaggccttg
     4561 ctggtttaac aattgcccct gcgttactgt cacttttggg ttgcaaacag gaagatattg
     4621 atagcggcac agtagggttg ataaatacac ccaaaggagt gctggtgacc caacgtgctc
     4681 gatgcacggg ttgccaccgc tgtgaaatct cttgtaccaa cttcaacgat ggctcagtag
     4741 ggacattctt ctcccgtatc aaaatccatc gcaattattt ctttggcgat aacggggttg
     4801 gctctggtgg tggcctgtat ggcgatctca actataccgc ggacacctgc cgtcaatgca
     4861 aagaaccgca atgcatgaat gtctgcccga ttggtgcgat tacctggcaa cagaaagaag
     4921 gctgtattac cgtcgatcat aaacgttgta ttggctgtag cgcctgtacg acggcgtgcc
     4981 cgtggatgat ggccaccgta aataccgaaa gtaaaaaatc ctcgaaatgt gtgttatgcg
     5041 gtgaatgcgc aaacgcctgc ccaacagggg cgttaaaaat aatcgagtgg aaagatatta
     5101 ctgtgtgaat cttgcaaagg aaaaaatcat ggctaacggt tggacaggta atatattaag
     5161 agtcaatctc acgacaggaa atattaccct cgaagattcc agtaagttta aaagttttgt
     5221 cggtggtatg ggcttcggct acaaaattat gtatgacgaa gtaccgccag gcacgaaacc
     5281 tttcgatgaa gcgaataaat tagtctttgc taccggccca ttaactggat ctggtgcccc
     5341 ctgtagttct cgtgtaaata tcacctcact ttctactttt accaaaggaa atttagtcgt
     5401 cgatgcccat atgggtggct tttttgcagc gcaaatgaaa ttcgctggct acgacgtcat
     5461 tattatcgaa gggaaagcga aatcaccggt atggctgaag attaaagatg acaaagttag
     5521 cctggaaaaa gccgatttct tatggggaaa agggacgcgc gcaacgacgg aagaaatttg
     5581 tcgcttgacc agtccggaaa cctgtgtggc ggctattggt caggctgggg aaaaccttgt
     5641 tcctctctct ggcatgttga atagccgtaa ccacagcggt ggtgcgggaa ctggcgcaat
     5701 aatgggttcg aaaaacctga aagcgattgc ggttgaaggg acgaaagggg tcaacattgc
     5761 cgatcgtcag gagatgaagc gtctcaacga ttacatgatg actgaactta tcggtgcgaa
     5821 taacaaccat gtcgtgccaa gtacgccgca atcgtgggca gagtattcag atcccaagtc
     5881 acgttggaca gcacgtaaag ggctgttttg gggcgcggct gaaggtggtc cgattgaaac
     5941 gggggaaatt ccgccaggca atcagaatac ggtcggcttt cgtacctata aatccgtttt
     6001 tgacttaggt ccggcggcag agaaatacac agtaaaaatg agcggctgcc actcttgccc
     6061 gatccgttgt atgacccaaa tgaatattcc tcgggtgaaa gagtttggcg tgcctggcac
     6121 cggtggtaac acttgtgtag caaactttgt ccataccacc atcttcccaa acgggccgaa
     6181 agattttgaa gataaagacg atggtcgtgt gattggtaac ctggttggtc tgaatctatt
     6241 cgatgactac ggcctatggt gtaactatgg gcagctgcat cgcgacttta cttattgtta
     6301 cagcaaaggg gtgttcaagc gtgttctacc agctgaagag tatgcggaaa ttcgctggga
     6361 tcaactggaa gcgggtgacg ttaacttcat taaagatttt tactaccgtc tggcgcatcg
     6421 tgtgggtgag ctgagtcacc tggctgatgg ttcatatgcc atcgcagaac gctggaattt
     6481 gggtgaagag tactggggct acgcgaaaaa taaactctgg tcgccgtttg gctatccggt
     6541 tcaccatgcc aatgaagcgt cagcgcaggt cggttccatt gttaactgta tgttcaaccg
     6601 tgactgcatg acgcataccc atatcaactt tattggttcc ggcttgccat tgaaactgca
     6661 acgtgaagtg gcgaaagaac tttttggttc tgaagatgct tacgacgaaa ccaaaaacta
     6721 cacgccaatc aatgacgcaa aaattaaata tgctaagtgg tcgctgctga gggtctgttt
     6781 gcataacgcc gtcaccctgt gcaactgggt ctggccaatg accgtttcgc cgctgaaaag
     6841 ccgtaattat cggggcgatc tggcgtttga agccaaattc ttcaaagcga tcactggcga
     6901 agatatgact caggaaaaat tagatttagc tgcagagcgt atctttacgt tgcatcgcgc
     6961 ctacacggta aaactgatgc aaaccaaaga tatgcgtaat gaacacgatc ttatctgttc
     7021 ctgggtattc gacaaggatc cgcagatccc ggtctttact gaaggtactg acaaaatgga
     7081 tcgtgacgat atgcatgctt cgctcactat gttctacaaa gaaatgggct gggacccaca
     7141 gcttggttgt ccgacccgcg aaacattgca gcgtctgggg ctggaagata ttgccgtcga
     7201 tctggcagca cacaatctac tgcccgcgta aggaatggga gagatgaacc atcaggatga
     7261 attgccgtta gcggaagtca gtgaaattga tgaggcgaag cggcaatggt tgcaggggat
     7321 gcgtcacccc gttgatacgg taaccgaacc agaaccggct gaaatcctgg cagagttcat
     7381 tcgccagcat tctgcggctg ggcagctggt ggcgcgcacc gtgtttctct cgccacccta
     7441 ttcggttgca gaagaggaac tgccagtttt gctggaaaac attaagcaga acggagacta
     7501 cgcggatatc gcgtgtatga cgggaagcca ggatgattac tattactcaa cgcaggccat
     7561 gagtgaaaac tacgcggcga tgtctttgca agttgtggag caggacattt gtcgtgccat
     7621 tgcacacgcc gttcgttttg agtgccagac gtatcctcgt ccttacaaag tcgccatgct
     7681 gatgcaagcg ccatattact tccaggaagc gcagattgaa gccgcgatcg ccgcgatgga
     7741 tgttgcacct gaatatgccg atatacggca ggtggagtca tcgacggcgg tgttgtactt
     7801 gtttagcgag cgatttatga cctatggcaa ggcgtatggc ttatgcgaat ggttcgaggt
     7861 agagcagttt cagaacccct gaaatttcag ggagatgtca tggatagggt ggacagttgc
     7921 agccactgca ttaggggata atcagatgtc cttcactcga cgcaaatttg ttctggggat
     7981 gggaacagta atctttttta ctggttctgc gtcttctctg ttagcgaaca cgaggcaaga
     8041 aaagaaagtt cggtatgcca tgattcatga cgagtcacgg tgcaatggct gtaatatttg
     8101 cgcccgcgca tgccgtaaaa ccaatcacgt ccccgctcag ggaagccgtt tatcgatagc
     8161 acacattcca gtcaccgata acgacaacga aacgcagtat cacttttttc gccagtcatg
     8221 tcagcactgc gaagatgctc cgtgcatcga cgtttgtccg acagcggcat cgtggcgtga
     8281 tgagcagggg atcgtacggg tagaaaaatc gcagtgcatc ggctgcagtt attgcattgg
     8341 cgcttgtcct taccaggttc gctaccttaa ccctgtcact aaagtggcag ataaatgtga
     8401 tttctgcgct gagtcccgac tggcaaaagg ctttccgccc atttgcgtca gcgcctgccc
     8461 ggaacacgcc ttgatctttg gtcgcgaaga tagcccagaa attcaggcgt ggttgcagga
     8521 aaataaatac tatcaatatc agctacctgg tgccgggaaa ccgcacctgt atcgccggtt
     8581 cggtcaacat ttgattaaaa aggaaaatgt atgaacccgt cgcaacatgc tgaacagttt
     8641 cagagccagt tggcgaacta tgtgccacag ttcactcccc aattttggcc ggtgtggttg
     8701 atcattgccg gactattgct ggtagggatg tggttggtgc tggggctgca tgccttgctt
     8761 cgtgctcgtg gcgtgaagaa atcagccacc gaccatggtg agaaggttta tctttacagc
     8821 aaagcggtca gattatggca ctggtcgaat gcgttactct ttgtattgtt gctggcaagt
     8881 gggctgataa atcactttgc gctggtgggc gcaactgcgg ttaaaagtct ggttgcggtg
     8941 catgaagttt gcggattttt gttactggca tgctggcttg gctttgttct gatcaatgcc
     9001 gttgggggta atggtcacca ctatcgcatt cgtcgtcagg ggtggctgga gcgagcggca
     9061 aaacaaacgc gattctattt gtttggcatt atgcaggggg aagaacatcc tttcccggca
     9121 acaacccagt ctaaatttaa tcccttacag caggttgcct atattggtgt catgtatgga
     9181 ttgctgccgt tgttactatt gacggggctg ctgtgtctct atccgcaagc cgtgggagat
     9241 gtgtttcctg gcgtaagata ctggttattg caggcgcatt ttgctctggc atttataagt
     9301 ctctttttta tcttcggtca tctttatctt tgcaccacgg ggcgtacgcc acacgaaacc
     9361 tttaaaagca tggtcgatgg ctatcaccgg cactaacgaa tgatcattac acgagcagac
     9421 ctgcgagaat ggcgtatcgg agcggtgatg tatcgctggt tcctgcgcca tttcccacgc
     9481 ggcggtagtt atgccgatat tcatcatgca ctgattgagg aaggatatac ggactgggcg
     9541 gagagtctgg ttgagtatgc gtggaaaaaa tggctggcgg atgaaaactt cgcgcatcag
     9601 gaagtttctt ctatgcaaaa actggccaca gatcccggag agagagcatt ttgcagtcag
     9661 tttgcccgca gcgacgatca tgctcgcatc ggctgttgtg aggataatgc cagaatcgca
     9721 accgctgggt atgccgcgca aattgccagt atgggttata gcgtgcggat tggaagtgtc
     9781 ggctttaaca gtcacattgg cagctccggt gaacgcgcac gggttgctgt aaccggtaat
     9841 tcttcgcgga taagcagcgc tggagatagt agtcggattg cgaataccgg aatgcgagta
     9901 cgcgtttgta cattgggtga acggtgtcat gtcgccagta atggtgatct ggtgcaaatc
     9961 gccagctttg gcgcaaacgc cagaattgcc aacagcggcg acaatgtaca catcattgcc
    10021 agcggtgaaa attcaacggt agtgagcacg ggcgtggtcg actcaattat tctcgggcca
    10081 ggaggaagcg ccgcactggc atatcatgac ggtgaaagag ttcgcttcgc cgtcgccatt
    10141 gaaggtgaaa acaatattcg agccggagtc cgctatcgtc ttaatgaaca gcatcagttt
    10201 gttgagtgct gatagcttta cctttaatca aacgaaagac gtttacgtgc acgggaggca
    10261 ggttttacga cagcccgagc catcgcctca ccaatttctg cacatgccgt aagtccctgt
    10321 acgaatggct ggtcgtgcat caaccagggc aacgcgccga acgctacgcg cccacgaatt
    10381 tcttcgggtt gctgtaacgt ataatcttcg ccaacatcag ggatttcatc ccctgttttc
    10441 tgtaattgtt cgcgtagtcc agggaaagga atatctttca ctttaagcgg acgctgcccg
    10501 cgggcatcaa taaaaacgtc aaacgagtag ctattgtctt ccgttttcag gacggtgcgc
    10561 gactcattaa tttccatttc gtagtcttca ccgagggcga gaatatgaat gattcccgct
    10621 tcacgtaagg ccagcagccg acgaatagac tctgaaggga ttgccgcata attatctatg
    10681 aaaactcggg caaggccttt actgaaccgt ttagttcatt cagatgtgga acaatttcct
    10741 gtactgcttc atgcaggcgc agaatgacat aacgccaggg aacagtatgt ttttctcgtt
    10801 tattgcgttc gacttcctgg agattttttt ctgcccagtc aaatgggtcg cgttgtttgc
    10861 gctcggcaaa ccaggcttga gcaaaggaat caacattcag gctctctaag gctatgcgtt
    10921 ggctccagtc tggatcagca aacttgattt cctctactat caatctaaat acccgatcca
    10981 aaaggccctt ttcgcctttt tgaatctcag catttaatgc ctgatcagtg acgatgtgta
    11041 agggctcgta gggaataggg caatagaaat cggcttcggg taaaataccc gtgcgcgaca
    11101 ttaacgtaat atttagcttt tcacttgcgt tatcgcggtg aaaaatgacg tgttgtttat
    11161 catcttcaat gaacgaacca tgc
//
LOCUS       DAMZFT010000150        11128 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.150, whole genome shotgun sequence.
ACCESSION   DAMZFT010000150 DAMZFT010000000
VERSION     DAMZFT010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11128)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11128)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11128
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.150"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(1..536)
                     /locus_tag="PN977_004225"
                     /pseudo
     CDS             complement(1..536)
                     /locus_tag="PN977_004225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001608690.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(524..1754)
                     /locus_tag="PN977_004226"
                     /pseudo
     CDS             complement(524..1754)
                     /locus_tag="PN977_004226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611657.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            2100..3033
                     /locus_tag="PN977_004227"
                     /pseudo
     CDS             2100..3033
                     /locus_tag="PN977_004227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001395480.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            3032..3525
                     /locus_tag="PN977_004228"
                     /pseudo
     CDS             3032..3525
                     /locus_tag="PN977_004228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001028172.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            complement(3750..3866)
                     /locus_tag="PN977_004229"
                     /pseudo
     CDS             complement(3750..3866)
                     /locus_tag="PN977_004229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012539895.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            4787..6874
                     /gene="iha"
                     /locus_tag="PN977_004230"
     CDS             4787..6874
                     /gene="iha"
                     /locus_tag="PN977_004230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional siderophore receptor/adhesin Iha"
                     /protein_id="HDI6044200.1"
                     /translation="MRVTTLASVAIPCLGFSVSSMAAEDVMVVTASGYEKKLTSAAAS
                     VSVISQEDLRSSQYHDLGEALRSVEGVDVESGTGKTGGLEISIRGMPASYTLILIDGV
                     RQGGSSDVTPNGYAAMNTGFMPPLAAIERIEIIRGPMSTLYGSDAMGGVVNIITKKNA
                     DKWRSSVNAGLNLQESNKWGNSSQFNFWSSGPLVDDSLSLQVRGSTQQRQGSSVTSLS
                     DTSATRIPYPTESQNYNAGGRLDWKATDKDVLWIDMDIARQRYDNRDGQLGSLTGGYD
                     RTLRYERNKISAGYDHTFTFGAWKSSLNWNETENKGRQLVRSVLQRDKWGLAGQPREL
                     KESNLILSSLLFAPLGESHKVTVGGEYQNSSMKDGVVLASTGETFRQKSWSAFVEDEW
                     YITDAFALTAGSRYERYEVFGGHFSPRAYMVWDVTDAWTLKGGVTTGYKAPSMGQLHK
                     GISGVSGQGRTNLLGNPDLKPEESVSYEAGVYYDNHAGLNANITGFMTEFSNKIVSYS
                     IDDNTRSYTNSGKARTQGVEFAGTLPLLSEDVTLSLNYTWTQSEQRDGDNKGAPLSYT
                     PEHMVNAKLNWQITEEVTSWLGARYRGKTPRFTQNYSSLSAVQKKVYDEKGEYLKAWT
                     VLDAGMSWKVTDALMLNAAVNNVLNKDYSDVSLYRAGSGTLYAGDYFQTGASTTGYVI
                     PERNYWMSLNYQF"
     gene            7808..8086
                     /locus_tag="PN977_004231"
     CDS             7808..8086
                     /locus_tag="PN977_004231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000285581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1525 domain-containing protein"
                     /protein_id="HDI6044201.1"
                     /translation="MYRIFCSLFFLPLAASAGTTIYTDSQHLPVNPPDGVRVVLLDAP
                     EQLQSRFWGLLPADAGEAESVVRVRMKSPEWQTMQAELAGHYRDVAHA"
     gene            8090..8203
                     /locus_tag="PN977_004232"
     CDS             8090..8203
                     /locus_tag="PN977_004232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008103180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1525 domain-containing protein"
                     /protein_id="HDI6044202.1"
                     /translation="MGVKKYPAVVFDDSEVVYGTMDVVMAGKIRAEGGVQP"
     gene            8200..9174
                     /locus_tag="PN977_004233"
                     /pseudo
     CDS             8200..9174
                     /locus_tag="PN977_004233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000987808.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03756 family integrating conjugative element
                     protein"
     gene            9181..9922
                     /locus_tag="PN977_004234"
                     /pseudo
     CDS             9181..9922
                     /locus_tag="PN977_004234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001681776.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrating conjugative element protein"
     gene            9917..10309
                     /locus_tag="PN977_004235"
                     /pseudo
     CDS             9917..10309
                     /locus_tag="PN977_004235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611134.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
     gene            10541..10992
                     /locus_tag="PN977_004236"
                     /pseudo
     CDS             10541..10992
                     /locus_tag="PN977_004236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004944208.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SgcJ/EcaC family oxidoreductase"
BASE COUNT         2908 a         2431 c         2913 g         2876 t
ORIGIN      
        1 ttcagtcggg cgacttcctc ccgcaacgca tcgcgttctg cctctaggga aggttcgtta
       61 tgtttacgca tagtctggta agcactgttg ccgataattt catctttcca tttatacaat
      121 accgcacgac tgacaccaat acgcctagag atttcactgg ctgatatttg tcgtgtacag
      181 aatgccatga cagcctgacg tttgacttct ggttcaaagg gcgcaaccgg actggtctta
      241 ctggtaaata tacgtcgtct gccggggtaa cgttcattaa cccagcgagc gagcacatcg
      301 gtacagggat aacccagagt tctactggta aaagctaggc agcagccgtg gttaagataa
      361 tgttcaacgg caacttgttt ttgctcatct gtgtaacggg gcttatggcg aatggattct
      421 tttgctccgc cacctgattc ctatgaacgg atccaacgcc gcagatttct tttcgaaggg
      481 tagcccagtt cgcgtacgac gggagccagc ttcttaccat tgactagtct tggtataagt
      541 tgacactttt ctgtctgtaa aacgcctgat gaacgtcata gggcgttttg tatttcagag
      601 ccaggtgtgg ccgctcatgg ttataaattg ccacggactc ttggaccatt tcccgggcct
      661 gagccaggtc tgcaggacgc gagagtaaaa actcattttt cagggtacca tttattcact
      721 cagccaatgc tggtagcagt cataaccatc agtcattgaa caggttattt catttctttc
      781 atgaactgac tgatagagcg ctgaacagta ttgcagcccc tgccagaatg atgcaccagg
      841 ggactcgtcg tttttctcag cttcagcacc tgtctgaacg cttttaccac attttctgtc
      901 tgcaggtttt ccccaacatg gtagcccata atttttctgg agcaggcatc ggtgaccaga
      961 ctcagatagg ccgaactatt gcgcagtgga aggtaagtaa tatcggcgac ccggacctgt
     1021 tctggttcaa gagcagtaac ctgttcaggg cctggtttaa gcaggtttgg atgtcggtag
     1081 aagcgatgat ggctgttggt agttttgtga tatgaccgtt ttactggcac caggagtcgg
     1141 tattcatcgg gcaggttaaa caggcggtct cgcccgatat tcagcctttt atcggcctga
     1201 gtgttcagca gatagtgcag cttacgcgca ccgattcggg gctgatgaca tctgacacgg
     1261 gcaacaaaat caagaacctg agcatgatgt tcctgccgtt ttctgcgcct tgcattgtcc
     1321 tgataccatg cctgtctgct gtgtcccagg aacagactgc acgcgtcaca gttattttgg
     1381 ggaccggact ttgcgcgagg acttgccggg ccgcttttta cgatactcac cccgtaatca
     1441 tttttcagga cattgataac tgactcaaaa aacgtagctt tctggttcgc cagctcaagc
     1501 tacgtttcaa gttccctgat tctttgctcg ggtgtgagcg ggatagttgt ctgagtcaca
     1561 ggcagtttcc tcttcaccag ctcaggaagt ccaggatatt tacgtaacca gacaagaacg
     1621 gtggagcgcc cctgaatgcc ataccgctgc tgggcctgtt tataagtcat ctcgcctttt
     1681 tcaacctgct caacaacagc tattttaaag gatagaggat aatcacgttg ggtgcgtttg
     1741 agctaagtca tcattacgtt ctccggattt aagtaggaac gtgtcaacgc tattcaggac
     1801 gggtcacata tttgaaataa agctcaatgg cgcggatttt ttcttcttca gtaaacatgt
     1861 ttcatctcct gagagtccag gaaatcgtcc gcatccccat tactgtctta tatcattggc
     1921 gctgacagct attctgctga gccttgagga tgcataaacg ggttaagagc ttgtatgtgc
     1981 agaacgatag cggaactaaa tagctgcgcg gaatagtaga tcactttgag ggagctcagc
     2041 ctggattgtg cgatctgatc aatcgccaaa tcaaaacaaa tcaccaaccg gactgagcaa
     2101 tgccgatcat agcaccaatt tcccgtgacg aacgacgcct gatgcagaaa gccatccata
     2161 aaacacacga taaaaattat gcccgcagac tgactgccat gctgatgctg caccggggcg
     2221 accgtgtcag cgacgttgcc agaacgctct gctgcgcccg ttcctctgtt ggatgttgga
     2281 ttaactggtt cacgcagtcg ggtgttgagg gactgaaatc attacctgcc gggcgagccc
     2341 gtctctggtc gtttgagcat atctgcacac tgttacgtga gctggtaaaa cattctcccg
     2401 gcgactttgg ctaccagcgt tcacgctgga gtacagaact gctggcaata aaaatcaatg
     2461 agataaccga ttgccagtta aatgccggaa ccgttcgccg ctggttgccg tctgcgggga
     2521 ttgtgtggcg aagggctgcg ccaactctgc gtatccgtga cccgcataaa gatgaaaaga
     2581 tggcagcaat ccataaagca ctggacgaat gcagcacaga gcatccggtc ttttatgaag
     2641 atgaagtgga tatccatctt aatcccaaaa tcggtgcgga ctggcaactg cgcggacagc
     2701 aaaagttcgg cgctgttcat cagcctgctg aagcggctta aagcgacata ccttcgggtg
     2761 aaaaccatca cactgatcgt ggacaactac attatccaca aaagccggga aacacagcgc
     2821 tggttgaagg agaacccgaa gttcagggtc atttatcagc cggtttactt gccatgggtg
     2881 aatcatgttg aacggctatg gcaggcactt cacgacacaa taacgcgtaa tcatcagtgc
     2941 cgctcaatgt ggcaactgtt gaaaaaaatt cgccatttta tggaaaccat cagcccgttc
     3001 cccggaggca aacatgggct ggcaaaagtg tagtggtatt aggcgcagct atttagatga
     3061 tctctttaac gatctcggtc gtagtataaa cttttttgat aaataaatag agacctgtcc
     3121 aggcaatctg aatgctgtca gcgtattgca aaagctaaag gcattggtcc tgaaattgtc
     3181 acgagcgttg ttgctgttat tattggcaaa ggcactgaat ttaagaatga tcgtcactgt
     3241 gctgcatggc ttggtctgct tccatgcctg cactagagtt gctgcaggta agttctcatg
     3301 aatataacga aaaaaggcga caagcatctg caaacacttt ttattcatgg cgtccgtgct
     3361 gtcgttagag ttgctacgaa taataatgat gtatacctaa atcagtgggt taagcagtta
     3421 aaggagagtc gcgaatttaa taaaactact gtggagattg ctaacaaata cgcgagaata
     3481 atctggtcga tgctgataaa tgatacagaa tatcaggcag tgtgaagtta atctctttca
     3541 gcaaaagttg cagcgtactg agaaatggtg acgggttgca cctgcacaat cggaacctga
     3601 tttttgtact ggcctcagag gtcgtccagt tgtagaggcg attgtgtgtg gattccctat
     3661 ttgggcacag gttttcctga tgccggatag atgtaagcaa caatccaaaa ccagaatcag
     3721 tgcttgcaaa actgaggtag tcaatagatg tagtaagtgg cttcagaaat accggcttcc
     3781 cgacaaacat ctttaattgt tcgtccggtt tcaaccgatt taattacagc gatgatctaa
     3841 tactcagtaa tacgggcttt acgcatagcg attttcttcg ttatcagatt gattattccg
     3901 gatgatctct aaatatgaat ggagcgatta tgcgggatac ttacagatgg ctctcactta
     3961 atgagagtct tctttcaaac aacaaaaaca gtaaattggg tagaatgggg aacttaactg
     4021 catcgttaat taatatatgg atgcgctgtt gtaaaatata gatgtatcgg ggataataat
     4081 actttactct gtttccagcg agtatgtttg gtttgctgga caaagcgcct cataaagtca
     4141 gaggcgctca aagtagaaat atatttcaga gggaatgtat cataatgtac tagtggttct
     4201 ttgatggtat atcaaaaatg tttgtgtgaa ctgtataaca gccctccagt agtgatgcta
     4261 gccatttgaa agatttctgc tgttctgaag accatctgga attatttgat tcaaaatgaa
     4321 tgaatgccag tatttgtatc atctgttcct taggaagcga tgaagccaga tatgaaaatg
     4381 caaccgtgag agcattcatg tcgtctcgaa gcattgacat gtactcgagt aattcctgca
     4441 gagtaattgt tctattgtcc ataaaactcc tcattattaa ttatattcac ctgtctgatg
     4501 tacgaaatat ccgatagtgt atgattaaca tatccaagga caagaaactg ataaagattg
     4561 gataaatacc catagtgcaa aaatccgata tggatcgtca acgattgcta tccgttactt
     4621 tcccctagag aaaagaacgt cctcaactga aattcggggt atagtagaac cttatgtaat
     4681 attttgtaat tttttgcttt tttatgtaaa aaccaaaagc aaatcattct cattatctct
     4741 tgcgtttgtt tatcttctct ctgattaata taatcggaga gaagatatgc gagtaaccac
     4801 tctggcttca gtagccatcc cttgtctcgg gttttcagtc agcagtatgg ctgcggaaga
     4861 tgtgatggta gtcacggcgt ctggctatga aaaaaaatta accagtgcag ctgcgagtgt
     4921 ttcagtgatc agccaggagg atttgcgatc cagccagtat catgatctgg gggaggcact
     4981 gagatccgta gagggggtgg atgttgaaag cggtacgggt aaaacaggtg ggctggaaat
     5041 cagcatccgc ggaatgcctg ccagttatac gctgatactg atcgatggtg tccgtcaggg
     5101 cgggagcagt gacgtaaccc caaacggtta tgctgccatg aatacgggtt ttatgccacc
     5161 tctggccgcc atcgaacgta ttgagattat caggggcccc atgtccactc tgtacggctc
     5221 tgatgccatg gggggcgtgg tgaatatcat caccaaaaag aatgcagaca aatggcgctc
     5281 ttcggttaat gccgggctga acctgcagga aagtaacaaa tgggggaaca gcagccagtt
     5341 taatttctgg agcagcggtc cccttgtgga tgattcactc agccttcagg tacgtggcag
     5401 cacacagcag cgtcagggct catcggtcac atcactgagc gacacctcag ctacgcgtat
     5461 cccttatccc acggaatcac agaattataa tgcaggtggt cgtcttgact ggaaagcgac
     5521 ggacaaggat gtactctgga ttgatatgga tatcgcccgg cagcgttatg ataaccgaga
     5581 tggccagctg ggcagtctga cgggcggtta tgatcgcacc ctgcgctatg agcgtaataa
     5641 aatttcagca ggctatgatc atacattcac cttcggagcc tggaagtcat ctctgaactg
     5701 gaatgagacg gaaaataaag gccgtcagct tgtacgtagc gtactgcagc gtgataaatg
     5761 ggggcttgcc ggccagccga gggagcttaa ggagtcgaat cttatcctga gctcattact
     5821 gtttgcacct ctgggggaat cacacaaggt taccgtcggt ggtgagtatc agaactcctc
     5881 catgaaagac ggggttgttc tcgccagtac tggtgaaacc ttccgtcaga aaagctggtc
     5941 ggcatttgtt gaggatgagt ggtatatcac ggatgcattt gcactgactg cgggtagccg
     6001 ctatgaacgc tatgaggtgt tcggaggaca cttcagtccc cgggcatata tggtctggga
     6061 tgtgactgat gcgtggacgc tgaaaggtgg ggtgaccacg ggatataagg cgcccagcat
     6121 ggggcagtta cataagggga tcagcggtgt gtccggtcag gggcgtacga atcttctcgg
     6181 taacccggac ctgaagccgg aggagagtgt cagttatgaa gcgggcgtgt attacgataa
     6241 tcatgccggt ctgaacgcca atatcacggg ctttatgaca gaattctcca acaagatcgt
     6301 atcttattcc attgatgata atacccgcag ctacacaaac agcggaaagg ctaggactca
     6361 gggcgtggaa tttgccggca cactgcctct gttgtcagag gatgtgactc tgtctctgaa
     6421 ttatacctgg acgcagagtg aacaacggga tggggataat aaaggtgcac cactgagtta
     6481 cacccctgag catatggtga atgcgaaact gaactggcag attactgaag aggtgacatc
     6541 atggctgggt gcccgttacc gcgggaaaac gccacgtttc acccagaatt attcgtcact
     6601 ctcagctgta cagaagaaag tgtatgatga gaaaggtgaa tatctgaaag cctggacggt
     6661 actggatgcg ggcatgtcgt ggaaagtgac ggatgcactg atgctgaatg cagcggtgaa
     6721 taatgtgctt aacaaggatt acagtgacgt aagtctgtat cgtgccggca gtggtacgct
     6781 ttatgcgggt gattacttcc agacgggggc atcgacaacg ggttatgtga tacctgagag
     6841 aaattactgg atgtcgctga actatcagtt ctgataacaa caaacccgat ataacgcatt
     6901 ataatacgta aagatgctgc ctctgtcacc tgagggcagt ggcagcaaca cacgatgcag
     6961 gtgtttgatc accagtgacc cgccgtttac tcctttagta tggcatactt ctgttgtcac
     7021 ctgtgaccag gcgcgcgact ctgtaaacat ccggatgtat gcgttcttct gacagttaat
     7081 attaccggca ggtagttgac gatgtgtggg cttgtgaccg catccgggta ataaagaaat
     7141 aatccgccag cggcttttat tcttatgggc agtaattaaa tacatcgcca tgtgggtaaa
     7201 tataagtgac ggaatgagag taacaagtga tgctggaatt aagattttgc tttttgcagt
     7261 gatagccaga gccttttaca gaaatgtggt atgaggtgca ttttttacgc tcacattatt
     7321 gttttattat cggcgtcaac cagtgatcag gaggtgcaac atgcgtatat gtgtttttgg
     7381 ttacaaaaca tgtccttcag tacaccggat catgcatcat gcgacaaatc tccgggatga
     7441 aacaggggca gatgtcctct ttattgatgc tgagaaacat ggtatcagtc tgaaggtaat
     7501 gtctgagcgt gctggatgtg cagttgaaaa attcccctgt gtttttgttg atgacgtcta
     7561 tctcgaacca ctgccaccgg tgccgggaca acctcattat acttattgtt gaaaatcacc
     7621 acttggtatg aatcagttcc ccggctttgt gaaaggccgg gtataatcac agactgtctg
     7681 ttatgcttgg cagatacact tccccggatc ccaccgtaat tttttaatcc cgtttttaat
     7741 ttattgctga taaatgagcg ctttcttcgc acgctgtcag tcagataata tctgactgag
     7801 gtgtacgatg taccggattt tctgttccct gtttttcctg ccgctggcag cgtctgccgg
     7861 cacaacaatt tataccgatt ctcagcatct tcccgttaat cctcctgatg gtgtcagggt
     7921 ggttctgctg gatgcgcctg agcagcttca gagccgcttt tggggcctgt tgcctgctga
     7981 tgcgggtgag gctgaatcgg tggtcagggt acgtatgaag tctcccgaat ggcagacgat
     8041 gcaggcggaa ctggccggtc attaccgcga tgtggcacat gcctagtcgc tgggcgtgaa
     8101 gaaataccct gccgtggtgt ttgatgacag tgaggtggtt tacggcacca tggatgtggt
     8161 gatggccggg aaaatccgtg ctgagggagg agtgcagcca tgatgtccct gaaatgtcgt
     8221 ctgcgtaccg gcgtgctgtt actgagtgtg gcatcactgc ccctggcgtc tgtatcactg
     8281 aacaccgcca gcattatcgc cagtactgcg gcaccggact atatcagctg gcgtgtcagc
     8341 gggatttgtt actggcttta ctgcagcgcg tctggctgta cggtcagaat ttccgtgaag
     8401 gtgacacatt tcatcccgga agtggttatt tccacctata cggcaccggg tggtaatccc
     8461 tggaaagaga tgtcgctggt cagccggacc gcaggtggcc cggagaatgc catcactgac
     8521 ggcctgtccg ggctgagtgc cggcggggga aatccggcgg atatgaaaat acaggggaag
     8581 cgccgttcag caattcgttt caaatatgca gatgcgctgg gtcatccggc cacacagctt
     8641 atcggcggga tggtacccgg attcagctgt gattcaccgg tgacaccggc catggcctat
     8701 ttcctcagta ccctggacag catcgtctag cgaacgggat tacctgaatc cctgtatcca
     8761 gaggcacagg ttcccggcaa acgcgagatt ggcagctagg ccgataaaaa catgtggggg
     8821 agcgtttatc accggagtgg ttttgtgatg cagagcgatg atgaccgggc cgcggcggtg
     8881 ggggctcagc gggtggccga cattatcacg cggatggggg agtcgcatgt ctacagggag
     8941 gttaagggcg tgaaacgtga cggctactgg cccccggagg cggtggagga aaacaccggc
     9001 acccgcaatc acaaatggca gcgactgacg ccgtcagtgt cgcgttcctg tgccgttttc
     9061 ccggatggtg aacaccaggc ggcggagaac ggtaatgcag cctttgcact gtggcagcct
     9121 tacagctgct tcgaaaagcg cgggcagcga ttccttggca gtacgaattt ttaaggaacc
     9181 atgatgatga agatgaaaac aggtatggtg gcactgagtg tgatgctgta ctgcagtatt
     9241 ccggcgatgg ctgatcttct ttccctgccg aaactgaatg acagcacgtt cggttacggg
     9301 aaatccgtgg atggcgccgt atccgacaaa ctgttctata cgctgggtgg cggctcggtg
     9361 atctcacagt tggccacgcg cagcaacatg cagaagctgg gtatggacct gggctggagc
     9421 agcgatctga tgtgcggcaa ctttgacctg aagaccacgg tgggtaatca tcttaacgga
     9481 ttgaccgacg gctttaaaaa tctgatgggg gatgtgattt agggggcgac cggcgccgct
     9541 gccagtctgc cggcgatggt gatacagcgt gccaaccccg gcctgtacga catgatcacc
     9601 aacggtgtgc tgcaggcgaa tgtggctttc gacaaggcgt aactgaactg ccagaacatg
     9661 gcaaaacgga tgatggactt ttctgatcgc agcaaatgga cgcaggccag cgatgctgga
     9721 agagtacaag aaggtggtga acagcggtga cggtgatgcg gtgaaagggg acgaggccgg
     9781 acgtcaggcc accgggaagg aaggtaagaa atggattggc gggcagcagc gcggcggggc
     9841 aggtcagccc gccatccggc cggtgcatga tctgacagcg gctgggttta acatgatgaa
     9901 cagtctgccc gtgaccagta cgggaggtta cgataaagcc gacagtgcag gatgtgatgt
     9961 taggagcgat agaaaaacgc tttggcgata aaggaccgga acgagcaatc cagtggcgga
    10021 cggataacgg ttcggcttat acggctcatg aaacacggca attcgccaga gagttgaatc
    10081 tggagccctg cacgacggca gtcagtagcc ctcagagcaa tagaatggca gaaaggttcg
    10141 tgaaaacgtg aatgaagact acatcgcgtt catgccgaaa ctgaatgtaa taacggcatt
    10201 gcataacctt gcagaggcgt tcgagcatta caatgaaaac catccgcata gtgcgttagg
    10261 ttatctctca ccacgggaat atcgacgtca gcggataacg ttaacttaag atacaatagc
    10321 agtctgatat tgagggggca agaacatccg aaccacacca gcctgtgact tataccctgt
    10381 ctctacgacc cagagagtat tattcttaat aatctcataa tatatacaca ataggaagta
    10441 tctattaaca aaatagagaa catcgttttt actatcaaat agttgcactg tattaatatg
    10501 attaaacttt aaaaaagttt caggctgatc aggaggaatt atgaaaaaaa taaatatttt
    10561 aattctgtgt ctgttttctg gagcgtcgtg ggcaagtgac gtagataatg ttaagtgtat
    10621 tcgggtagat agtaaagaca ttgaaaggtt attcgatcgc tggaatgaat cattaaaaac
    10681 aggtgatgcc aagaaggttg ctgataacta tttaagtgat gcgatcttac tgcccacagt
    10741 atcaaaaaaa attaggatta cagatgcaga aagagttgac tattttgagc attttttgaa
    10801 aaaaaacctg ttggagaaat aaacttcaga actattcgca ttggatgcaa tatggctctt
    10861 gatacaggga attacacgtt tacttttgaa gataaaaaac aagtttcagc acgctatagt
    10921 ttcgcttatg agtgggatgg aaaggattgg aaaatatcat cccatcattc atctgtagtg
    10981 ccaacaacat aatatattga gcctgtttac cattctctgt taatgccttc tcttaaaagt
    11041 gaccgtccaa gcggtcactg attacccatt cttaattcag tgcaggatta aaatatatgt
    11101 gcaaaataat ttaattcaga cagctttt
//
LOCUS       DAMZFT010000151        11123 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.151, whole genome shotgun sequence.
ACCESSION   DAMZFT010000151 DAMZFT010000000
VERSION     DAMZFT010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11123)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11123)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11123
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.151"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..390
                     /locus_tag="PN977_004237"
     CDS             <1..390
                     /locus_tag="PN977_004237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1479 family protein"
                     /protein_id="HDI6044203.1"
                     /translation="LLDDVPEDELCGVAPGRVLPVSEQWHPLLIEALTSIPQLEAGDS
                     VWWHCDVIHSVAPVENQQGWGNVMYIPAAPMCEKNLAYAHKVKAALEKGASPGDFPRE
                     DYEANWEGRFTLEDLNIHGKRALGMDV"
     gene            complement(631..2223)
                     /locus_tag="PN977_004238"
     CDS             complement(631..2223)
                     /locus_tag="PN977_004238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATPase"
                     /protein_id="HDI6044204.1"
                     /translation="MLVSSNVTMQFGSKPLFENISVKFGGGNRYGLIGANGSGKSTFM
                     KILGGDLEPTLGNVSLDPNERIGKLRQDQFAFEEFTVLDTVIMGHKELWEVKQERDRI
                     YALPEMSEEDGYKVADLEVKYGEMDGYSAEARAGELLLGVGIPVEQHYGPMSEVAPGW
                     KLRVLLAQALFADPDILLLDEPTNNLDIDTIRWLEQVLNERDSTMIIISHDRHFLNMV
                     CTHMADLDYGELRVYPGNYDEYMTAATQARERLLADNAKKKAQIAELQSFVSRFSANA
                     SKSRQATSRARQIDKIKLEEVKASSRQNPFIRFEQDKKLFRNALEVEGLTKGFDNGPL
                     FKNLNLLLEVGEKLAVLGTNGVGKSTLLKTLVGDLQPDSGTVKWSENARIGYYAQDHE
                     YEFENDLTVFEWMSQWKQEGDDEQAVRSILGRLLFSQDDIKKPAKVLSGGEKGRMLFG
                     KLMMQKPNILIMDEPTNHLDMESIESLNMALELYQGTLIFVSHDREFVSSLATRILEI
                     TPERVIDFSGNYEDYLRSKGIE"
     gene            2442..3362
                     /gene="ldtB"
                     /locus_tag="PN977_004239"
     CDS             2442..3362
                     /gene="ldtB"
                     /locus_tag="PN977_004239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HDI6044205.1"
                     /translation="MNMKLKTLFAAAFAVVGFCSTASAVTYPLPTDGSRLVGQNQVIT
                     IPEGNTQPLEYFAAEYQMGLSNMMEANPGVDTFLPKGGTVLNIPQQLILPDTVHEGIV
                     INSAEMRLYYYPKGTNTVIVLPIGIGQLGKDTPINWTTKVERKKAGPTWTPTAKMHAE
                     YRAAGEPLPAVVPAGPDNPMGLYALYIGRLYAIHGTNANFGIGLRVSHGCVRLRNEDI
                     KFLFEKVPVGTRVQFIDEPVKATTEPDGSRYIEVHNPLSTTEAQFEGQEIVPITLTKS
                     VQTVTGQPDVDQVVLDEAIKNRSGMPVRLN"
     gene            complement(3421..4539)
                     /locus_tag="PN977_004240"
     CDS             complement(3421..4539)
                     /locus_tag="PN977_004240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion transporter"
                     /protein_id="HDI6044206.1"
                     /translation="MSLPFLRTLQGDRFFQLLILVGIGLSFFVPFAPKSWPAAIDWHT
                     IITLSGLMLLTKGVELSGYFDVLGRKMVRRFATERRLAMFMVLAAALLSTFLTNDVAL
                     FIVVPLTITLKRLCEIPVNRLIIFEALAVNAGSLLTPIGNPQNILIWGRSGLSFAGFI
                     AQMAPLAGAMMLTLLLLCWCCFPGKALQYHTGVQTPEWKPRLVWSCLGLYIVFLTALE
                     LKQELWGLVIVAAGFALLARRVVLSVDWTLLLVFMAMFIDVHLLTQLPALQGVLSNVS
                     HLSEPGLWLTAIGLSQVISNVPSTILLLNYVPPSLLLAWAVNVGGFGLLPGSLANLIA
                     LRMANDRRIWWRFHLYSIPMLLWAALVGYVLFVMLPAN"
     gene            complement(4536..5003)
                     /gene="mntR"
                     /locus_tag="PN977_004241"
     CDS             complement(4536..5003)
                     /gene="mntR"
                     /locus_tag="PN977_004241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-binding transcriptional regulator
                     MntR"
                     /protein_id="HDI6044207.1"
                     /translation="MSRRAGTPTAKKVTQLVNVEEHVEGFRQVREAHRRELIDDYVEL
                     ISDLIREVGEARQVDMAARLGVSQPTVAKMLKRLATMGLIEMIPWRGVFLTAEGEKLA
                     QESRERHQIVENFLLVLGVSPEIARRDAEGMEHHVSEETLDAFRLFTQKHGAK"
     gene            5189..5317
                     /gene="mntS"
                     /locus_tag="PN977_004242"
     CDS             5189..5317
                     /gene="mntS"
                     /locus_tag="PN977_004242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganase accumulation protein MntS"
                     /protein_id="HDI6044208.1"
                     /translation="MNEFKRCMRVFSHSPFKVRLMLLSMLCDMVNNKPQQDKPSDK"
     gene            5589..7172
                     /gene="opgE"
                     /locus_tag="PN977_004243"
     CDS             5589..7172
                     /gene="opgE"
                     /locus_tag="PN977_004243"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HDI6044209.1"
                     /translation="MNLTLKDSLVTRSRVFSPWTAFYFLQSLLINLGLGYPFSLLYTA
                     AFTAILLLLWRTLPRVQKVLVGVSSLVAACYFPFAQAYGAPNFNTLLALHSTNMEEST
                     EILTIFPWYSYLVGLFIFALGVIAIRRKKENEKARWNTFDSLCLVFSVATFFVAPVQN
                     LAWGGVFKLKDTGYPVFRFAKDVIVNNNEVIEEQERMAKLSGMKDTWTVTAVKPKYQT
                     YVVVIGESARRDALGAFGGHWDNTPFASSVNGLIFADYIAASGSTQKSLGLTLNRVVD
                     GKPQFQDNFVTLANRAGFQTWWFSNQGQIGEYDTAIASIAKRADEVYFLKEGNFEADK
                     NTKDEALLDMTAQVLAQEHSQPQLIVLHLMGSHPQACDRTQGKYETFVQSKETSCYLY
                     TMTQTDDLLRKLYDQLRNSGSSFSLVYFSDHGLAFKERGKDVQYLAHDDKYQQNFQVP
                     FMVISSDDKAHRVIKARRSANDFLGFFSQWTGIKAKEINIKYPFISEKKAGPIYITNF
                     QLQKVDYNHLGTDIFDPKP"
     gene            complement(7221..7736)
                     /gene="ompX"
                     /locus_tag="PN977_004244"
     CDS             complement(7221..7736)
                     /gene="ompX"
                     /locus_tag="PN977_004244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpX"
                     /protein_id="HDI6044210.1"
                     /translation="MKKIACLSALAAVLAFTAGTSVAATSTVTGGYAQSDAQGQMNKM
                     GGFNLKYRYEEDNSPLGVIGSFTYTEKSRTASSGDYNKNQYYGITAGPAYRINDWASI
                     YGVVGVGYGKFQTTEYPTYKHDTSDYGFSYGAGLQFNPMENVALDFSYEQSRIRSVDV
                     GTWIAGVGYRF"
     gene            complement(7789..7854)
                     /locus_tag="PN977_004245"
     CDS             complement(7789..7854)
                     /locus_tag="PN977_004245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6044211.1"
                     /translation="METFCYMKWPVRHHKSRRVSH"
     gene            8089..8976
                     /gene="rhtA"
                     /locus_tag="PN977_004246"
     CDS             8089..8976
                     /gene="rhtA"
                     /locus_tag="PN977_004246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/homoserine exporter RhtA"
                     /protein_id="HDI6044212.1"
                     /translation="MPGSLRKMPVWLPIVILLVAMASIQGGASLAKSLFPLVGAPGVT
                     ALRLALGTLILIAFFKPWRLRFAKEQRLLLLFYGVSLGGMNYLFYLSIQTVPLGIAVA
                     LEFTGPLAVALFSSRRPVDFVWVVLAVLGLWFLLPLGQDVSHVDLTGCALALGAGACW
                     AIYILSGQRAGAEHGPATVAIGSLIAALIFVPIGVLQAGEALWHWSVIPLGLAVAILS
                     TALPYSLEMIALTRLPTRTFGTLMSMEPALAAVSGMIFLGETLTPIQLLALGAIIAAS
                     MGSTLTVRKESKIKELDIN"
     gene            9276..9779
                     /gene="dps"
                     /locus_tag="PN977_004247"
                     /gene_synonym="pexB"
     CDS             9276..9779
                     /gene="dps"
                     /locus_tag="PN977_004247"
                     /gene_synonym="pexB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001730644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein Dps"
                     /protein_id="HDI6044213.1"
                     /translation="MSTAKLVKSKATNLLYTRNDVSDSEKKATVELLNRQVIQFIDLS
                     LITKQAHWNMRGANFIAVHEMLDGFRTALIDHLDTMAERAVQLGGVALGTTQVINSKT
                     PLKSYPLDIHNVQDHLKELADRYAIVANDVRKAIGEAKDDDTADILTAASRDLDKFLW
                     FIESNIE"
     gene            10183..10929
                     /gene="glnH"
                     /locus_tag="PN977_004248"
     CDS             10183..10929
                     /gene="glnH"
                     /locus_tag="PN977_004248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter substrate-binding
                     protein GlnH"
                     /protein_id="HDI6044214.1"
                     /translation="MKSVLKVSLAALTLAFAVSSHAADKKLVVATDTTFVPFEFKQGD
                     KYVGFDVDLWAAIAKELKLDYELKPMDFSGIIPALQTKNVDLALAGITITDERKKAID
                     FSDGYYKSGLLVMVKANNNDVKSVKDLDGKVVAVKSGTGSVDYAKANIKTKDLRQFPN
                     IDNAYMELGTNRADAVLHDTPNILYFIKTAGNGQFKAVGDSLEAQQYGIAFPKGSDEL
                     RDKVNGALKTLRENGTYNEIYKKWFGTEPK"
BASE COUNT         2818 a         2878 c         2642 g         2785 t
ORIGIN      
        1 ctgcttgatg atgtgccgga ggatgaactg tgcggcgtag cgcccggaag agtgttgccg
       61 gtatcagagc aatggcatcc actgttgatt gaggcattaa ccagcattcc acaactcgaa
      121 gccggagact ccgtctggtg gcactgcgac gtcatccatt ccgttgcccc cgttgaaaat
      181 caacagggct ggggcaacgt gatgtacatt cctgcggcac cgatgtgcga gaaaaatctt
      241 gcctacgcgc acaaggtgaa ggccgcactg gaaaaaggcg catcacctgg cgacttcccg
      301 cgggaagatt atgaagcaaa ctgggaaggc cgctttacgc tggaggatct gaacattcac
      361 ggtaagcgcg cactgggcat ggatgtttga gagttaatgg aagtgaattg atgtgggatt
      421 aagaaaatcg attcgacgtt actcagattg ctgacaaatg cgctttgtta atgcctgatg
      481 cggcgttaac gccttatccg gcctacaaag tcctgcagat tcaatatatt gcaggtactg
      541 cgtaggcctg ataagcatag cgcatcaggc aatcttgcgt ttgtcatcag ccgccgtcac
      601 cccgacagta tcgggctgac ggataataac ttactcgatc cctttactac gcagataatc
      661 ttcgtaatta ccgctaaagt cgatcacgcg ttccggggtg atttccagaa tacgggtcgc
      721 cagggagctt acgaactcac ggtcgtgtga aacaaagatc agcgtgccct gatacagttc
      781 cagcgccatg ttcagcgact caatagattc catatccagg tggttggtcg gttcgtccat
      841 gatcagaatg ttcggcttct gcatcattaa cttaccaaac agcatccgcc ctttctcccc
      901 accggaaagc actttcgctg gctttttgat atcgtcctgg ctgaacagca aacgaccgag
      961 aatgctgcgt accgcctgct cgtcatcgcc ttcctgcttc cactggctca tccattcgaa
     1021 cacggtcaga tcattttcga actcatattc atggtcctgg gcgtagtaac caatgcgcgc
     1081 gttctcagac cattttacgg tgccgctgtc cggttgcaga tcgcccacca gcgttttcag
     1141 cagcgttgat ttaccgacgc cgttggtacc cagtaccgcc agtttttcac cgacttccag
     1201 cagcaggttg agatttttaa acagtggacc gttatcaaac cctttggtca gaccttccac
     1261 ttccagcgcg ttacggaaca gtttcttatc ctgctcaaaa cggatgaacg ggttctgacg
     1321 gctggaagct ttcacctctt ccagtttgat tttatcgatc tggcgcgcac gcgaagttgc
     1381 ctggcgagat ttcgaggcgt tggcgctaaa gcggctaacg aaagattgca gctcggcaat
     1441 ctgcgctttc ttcttggcat tatcagccag cagacgttca cgcgcctggg tcgccgccgt
     1501 catgtactca tcgtagttac ctggataaac gcgcagctcg ccgtaatcca gatccgccat
     1561 atgggtacaa accatgttaa ggaagtgacg gtcgtgcgag atgatgatca tggtgctgtc
     1621 acgctcgttc agcacctgtt ccagccaacg aatggtgtcg atgtcgaggt tgttggtcgg
     1681 ttcgtcgagc aggagaatgt ccggatcagc aaacagcgcc tgcgccagca gcacacgcag
     1741 cttccagcca ggagcaactt cactcatcgg gccgtagtgc tgttccactg gaattcccac
     1801 gccaagcaac agttcaccgg cgcgagcttc cgcagagtaa ccgtccattt cgccgtattt
     1861 tacttccaga tcggccactt tataaccgtc ttcttcgctc atttccggca aagcatagat
     1921 acggtcgcgc tcctgcttca cttcccacaa ctctttatgc cccatgatca ccgtatccag
     1981 cacggtgaac tcttcaaagg caaactgatc ctgacgcaat ttaccaatgc gctcgttggg
     2041 atcgagggaa acgttaccca gcgtcggctc taagtcgccg ccgaggatct tcataaaggt
     2101 ggatttacca ctaccgttcg cgccaatcag gccgtaacgg ttgccgccgc caaacttgac
     2161 ggaaatgttt tcaaacaacg gcttactgcc gaactgcatg gtgacgttac tggaaactaa
     2221 cacaggcgta tcctgaaaag agatatgaca aaccgcgtat tatgccataa gtctgaaaga
     2281 agatcccgtc ccccgcttaa ggtctacact cttcccaaag gcaaaaaatg tgatttcgta
     2341 cacatctgat ttcactgtga gctggaatga acttataatg cgcttccaat actctaatat
     2401 tctcaaccca atggcctgcc aggcacaaaa tctcgcttaa catgaatatg aaattgaaaa
     2461 cattattcgc agcggccttc gctgttgtcg gcttttgcag taccgcctct gcggtaactt
     2521 atcctctgcc aaccgacggg agtcgcctgg ttggtcagaa tcaggtgatc accattcctg
     2581 aaggtaacac tcagccgctg gagtattttg ccgccgagta ccagatgggg ctttccaata
     2641 tgatggaagc gaacccgggt gtggatacct tcctgccgaa aggcggtact gtactgaaca
     2701 ttccgcagca gctgatcctg ccggataccg ttcatgaagg catcgtcatt aacagtgcag
     2761 agatgcgtct gtattactat ccgaaaggga ccaacaccgt tatcgtgctg ccgatcggca
     2821 ttggtcagtt aggcaaagat acgcctatca actggaccac caaagttgag cgtaagaaag
     2881 caggcccgac ctggacgccg accgccaaaa tgcacgcaga gtaccgcgct gcgggcgaac
     2941 cgcttccggc tgtcgttccg gcaggtccgg ataacccgat ggggctgtat gcactctaca
     3001 tcggtcgcct gtacgctatc catggcacca acgccaactt cggtatcggc ctgcgtgtaa
     3061 gtcatggttg tgtgcgtctg cgtaacgaag acataaaatt cctgttcgag aaagtaccgg
     3121 tcggtacccg cgtacagttt attgatgagc cggtaaaagc gaccaccgag ccagacggca
     3181 gccgttatat tgaagtccat aacccgctgt ctaccaccga agcccagttt gaaggtcagg
     3241 aaattgtgcc aattaccctg accaagagcg tgcagacagt aactggtcag cctgatgtgg
     3301 atcaggtcgt gctggatgaa gcgatcaaaa atcgttccgg gatgccggtt cgtctgaatt
     3361 aatcgtcaaa acgtaaaaca gaggcggact catcatccgc cttttttatt tggccagacc
     3421 ctagttggcc gggagcataa cgaataaaac gtatcccacc agcgccgccc acaacagcat
     3481 cggtatcgaa taaagatgaa aacgccacca gatacggcga tcgttcgcca tccgtaacgc
     3541 aatcaaattt gccagcgatc cgggtaataa accaaagccc cccacgttta ccgcccatgc
     3601 cagcagcaac gacggcggca catagttcag caacaatatg gtactcggca cattactgat
     3661 cacctgcgat aaaccgattg ccgttaacca taatccgggt tcggataaat ggctcacatt
     3721 gctcaacacg ccctgcaaag ctggcagttg ggtcagcaaa tggacgtcga taaacatcgc
     3781 cataaatacc agcagcagcg tccagtccac actcaacacc acgcgacgtg ccagcagcgc
     3841 aaagcccgcc gccacaatca ccagtcccca cagctcttgt tttaactcca gcgccgtcag
     3901 aaagacgata tacagcccca gacaactcca caccagccgc ggtttccact ccggtgtttg
     3961 cacccccgta tggtattgga gtgcctttcc agggaaacaa caccagcaca acagcaggag
     4021 cgtcagcatc attgcgccag ccagcggtgc catttgggca ataaatccgg caaacgaaag
     4081 accagaacgt ccccagataa gaatattttg cgggttgcca attggcgtca gtagcgaacc
     4141 cgcattgact gccagcgcct caaaaataat cagccgatta accgggatct cacacagtct
     4201 ttttagcgtg atagtcagcg gaacaactat aaacagcgcg acatcgttgg tcagaaaggt
     4261 agaaagcagc gccgccgcca gcaccataaa catcgccagc cgacgctccg tagcaaagcg
     4321 gcgcaccatt ttgcgcccca gcacatcaaa ataaccgctt aactccacac ctttggtcag
     4381 cagcatcagg ccgcttaagg tgatgatggt gtgccagtcg atagcagcag gccaggattt
     4441 cggtgcaaag ggcacgaaaa agcttaatcc gataccaaca agaattaata actgaaaaaa
     4501 acgatcccct tgcagcgtgc gtaaaaaagg caggctcatt tggcaccgtg tttctgggta
     4561 aacaaacgaa aagcgtcgag cgtctcttca ctgacatggt gctccatgcc ttccgcgtcg
     4621 cgacgggcga tttccggact gacgcccaac accaacaaga aattttcgac tatctgatgt
     4681 cgctcgcggc tttcctgcgc cagcttctct ccttctgccg ttaaaaacac gccacgccag
     4741 gggatcattt caatcagccc catggttgcc agtcgcttaa gcattttagc caccgtcggt
     4801 tgcgaaactc ccaaacgagc agccatgtct acctgacgag cttcccccac ttccctgatc
     4861 aagtcagaaa tcagctcaac gtaatcatca ataagctcgc gccgatgcgc ctctctgacc
     4921 tggcggaacc cttcaacgtg ctcttccacg ttcactaact gcgtcacttt ttttgctgtt
     4981 ggcgtacctg cgcgacgact cattgtgctt cctcattatg gtgacgcata cctagcgttc
     5041 tcatttcgtg cgcacattgt aaaccagagt tgcgaaggta caaaaaatta acgttttagc
     5101 aatagctata taatatagcc tgtgctatat ctgtatgtaa tgcaatcatc cctcaaggat
     5161 cgacgggatt agcaagtcag gaggtcttat gaatgagttc aagaggtgta tgcgcgtgtt
     5221 tagtcattct ccctttaaag tacggttaat gctgctctct atgttgtgcg atatggtcaa
     5281 caacaaaccg cagcaagata aaccttccga taaatagcgg cgtcgcggta cgccgcttca
     5341 ctcctgcttt tatgcaggca caacgcgttt tggtctgaaa aaccccactt tttgtcggat
     5401 ttgcaatccc cttcgcgaaa gatttgttcg tcagtagttg acctgaacgg cggctcgctc
     5461 tatcttcttg cagccctgcg tatattgcgg ctcgcggatg cggacccctt tcccctcttc
     5521 acgcactctt gcaggtattg acccttgacg ccagggtaag cacatggcgt ttgttacgat
     5581 agtggcatat gaatttaacc ctcaaagact cgcttgttac ccgtagccgg gtatttagcc
     5641 cgtggactgc gttctacttt ttacagtcgc tattaattaa cctcggctta ggttatccct
     5701 tcagtttgct ctacaccgct gcgtttacgg ctattttgct tttgctatgg cgaacattgc
     5761 ctcgcgtaca aaaagttctg gtcggtgtca gttcgctggt ggcggcttgt tatttccctt
     5821 ttgctcaggc ctacggcgcg cctaatttca atacattgct ggcattgcac tccaccaata
     5881 tggaagagtc gaccgaaatc ctgacgattt ttccgtggta cagctacctg gtcggcttat
     5941 ttatttttgc gctcggcgta atagcaatca ggcgaaaaaa agagaatgaa aaagcgcgct
     6001 ggaatacctt cgacagcctg tgtctggtat tcagtgtggc gacatttttt gttgctcccg
     6061 tgcaaaacct ggcctggggc ggcgtattta aactgaaaga tactggctat ccggtatttc
     6121 gttttgctaa ggatgtcatc gtcaataata acgaggtgat cgaagagcaa gaacggatgg
     6181 caaaactttc cggaatgaaa gatacctgga cggtcactgc cgttaagccg aagtatcaga
     6241 cctatgtggt ggtgatcggt gaaagcgcgc gtcgcgatgc cctcggtgcc tttggcggtc
     6301 actgggacaa taccccgttt gccagcagcg ttaacggttt gatatttgct gactacattg
     6361 ccgccagtgg ctccacgcag aaatcgcttg gcttaacgct caatcgcgtt gtcgatggca
     6421 aaccacagtt tcaggataac tttgtcaccc tggcaaatcg cgcgggcttc cagacctggt
     6481 ggttttccaa ccagggccaa atcggcgaat acgataccgc catcgccagc atcgccaaac
     6541 gtgcagatga agtgtacttc ctgaaagaag gtaattttga agcagataaa aacacgaaag
     6601 acgaagcgtt actggatatg accgctcaag tgctggcgca agagcactcg caaccgcagc
     6661 tgattgttct acatctgatg ggctcgcatc cgcaggcctg cgacaggaca caaggcaaat
     6721 acgaaacctt tgtgcaatca aaagaaacgt cgtgctatct ctataccatg acgcaaacgg
     6781 acgatttact gcgcaaactg tacgatcagt tacgcaacag cggcagcagc ttctcgctgg
     6841 tttacttttc tgaccacggt ctggccttta aagagcgcgg caaagacgtg caataccttg
     6901 cccatgatga taagtatcag caaaatttcc aggtgccttt tatggtcatt tccagcgacg
     6961 ataaagcgca tcgggtgatt aaagcccgcc gctcagccaa tgacttctta ggttttttct
     7021 cccagtggac gggaattaaa gcgaaggaaa ttaacatcaa atacccgttt atatctgaga
     7081 agaaagccgg gccgatatac atcaccaact tccagttaca gaaggtagat tacaaccatc
     7141 tcggaaccga tattttcgac ccgaaacctt aaaaacaaaa atccgccccg agaggcggat
     7201 tttttatatc accgaagtga ttagaagcgg taaccaacac cggcaatcca ggtgcctacg
     7261 tcaacgctac gaatacggct ctgctcgtaa gagaagtcca gagcaacgtt ttccatcggg
     7321 ttgaactgca gaccagcacc gtaggagaaa ccgtagtcgc tggtgtcgtg tttgtaggtc
     7381 gggtattcag tggtctggaa tttaccataa cccacaccca ctacaccgta gatgcttgcc
     7441 cagtcgttaa tgcggtaagc cggaccagca gtgatgccgt agtactggtt tttgttgtag
     7501 tcaccagagc ttgcagtacg gcttttctcg gtgtaagtga aagaaccgat cacacccagc
     7561 gggctgttgt cttcttcata gcggtatttc aggttgaaac cgcccatttt gttcatttgg
     7621 ccctgagcgt cgctctgtgc gtaaccgcca gttacagtag aagtcgcagc tacggaagta
     7681 cctgcggtga aagccagaac tgcggccagt gctgaaagac atgcaatttt tttcataacc
     7741 acctcaaatg tgattcaaat aagtcctaag ttttaaatat atcaaaaatt aatgggaaac
     7801 tcttcgcgat ttatgatgtc taacgggcca tttcatgtaa cagaacgttt ccatacaccg
     7861 ctatccatct aaatttaaat cactttttca gagaactgcg taagtattac gcatgttttc
     7921 cctgtcattc atccagatta ttcctaatca ccagactaat gattccatca atcctggcgc
     7981 attttagtca aaacggggga aaattttttc aacaaatgct caaccagcat tgggtatatc
     8041 cagtacactc cacgctttac ttaggtttag atatttgtgg gagaaaggat gcctggttca
     8101 ttacgtaaaa tgccggtctg gttaccaata gtcatattgc tcgttgccat ggcgtctatt
     8161 cagggtggag cctcgttagc taagtcactt tttcctctgg tgggcgcacc gggggtcact
     8221 gcgctgcgtc tggcattagg cacgctgatc ctcatcgcgt tctttaagcc atggcgactg
     8281 cgctttgcca aagagcagcg gttactgctg ttgttttacg gcgtttcgct gggggggatg
     8341 aattatcttt tttatctttc tattcagacg gtaccgctgg gtattgcggt ggcgctggag
     8401 ttcaccggac cactggcggt ggcgctgttc tcttctcgtc gcccggtaga tttcgtctgg
     8461 gttgttctgg cggttcttgg tctgtggttc ctgctaccgc tggggcaaga cgtttcccat
     8521 gtcgatttaa ccggctgtgc gctggctctg ggggccgggg cttgttgggc tatttacatt
     8581 ttaagtgggc aacgcgcagg agcggaacat ggccctgcga cggtggcaat tggttcgttg
     8641 attgcagcgt taattttcgt gccaattgga gtgcttcagg ctggtgaagc actctggcac
     8701 tggtcggtta ttccattggg tctggcggtc gctattctct cgaccgctct gccttattcg
     8761 ctggaaatga ttgccctcac ccgtttgcca acacgcacat ttggtacgct aatgagcatg
     8821 gaacctgcgc tggctgccgt ttccgggatg attttcctcg gagaaacact gacacccata
     8881 cagctgctgg cgctcggcgc tatcatcgcc gcttcaatgg gttctacgct gacagtacgc
     8941 aaagagagca aaataaaaga attagacata aattaaattt acatttctgc atggttatgc
     9001 ataaccatgc agattttctc gctacttttc ctctacaccg tctttatata tcgaattatg
     9061 caaaagcata tttattccga aaattcctgg cgagcaaata aataagaatt gttcttatta
     9121 atatatctaa ctcattgagt ttttattggt tttgtttttt cacgcttgtt accactatta
     9181 gtgtgatagg aacagccaga atagcggaac acatagctgg tgctatactt agtctcgtta
     9241 attactggga cataacatca agaggatatg aaattatgag taccgctaaa ttagttaaat
     9301 caaaagcgac caatctgctt tatacccgca acgatgtctc tgatagcgag aaaaaagcaa
     9361 cagtagagtt gctgaatcgc caggttatcc agtttattga tctttctttg attaccaaac
     9421 aagcgcactg gaacatgcgc ggcgctaact tcattgccgt acatgaaatg ctggatggct
     9481 tccgcactgc actgatcgat catctggata ccatggcaga acgtgcagtg cagctaggcg
     9541 gtgtagctct ggggaccact caagttatca acagcaaaac cccgctgaaa agttacccgc
     9601 tggacatcca caacgttcag gatcacctga aagaactggc tgaccgttac gcaatcgtcg
     9661 ctaatgacgt acgcaaagcg attggcgaag cgaaagatga cgacaccgca gatatcctga
     9721 ccgccgcgtc tcgcgacctg gataaattcc tgtggtttat cgagtctaac atcgaataaa
     9781 tccatcgctg atggtgcaga actttagtac ccgataaaag cggcttcctg acaggaggcc
     9841 gttttgtttt gcagcccacc tcaacgcact tatttagtgc atccatctgc tatctccagc
     9901 tgattaagta aattttttgt atccatatca tcacacaatc gttacataaa gattgttttt
     9961 tcatcaggtt ttacgctaaa taatcactgt gttgagtgca caattttagc gcaccagttt
    10021 ggtgccccag aatggtgcat cttcagggta ttgccctata aatcgtgcat cacgttttta
    10081 ccgcatctcg ataaatcaat gagttgcaaa actggcacga ttttttcata tatgtgaatg
    10141 tcacgcaggg gatcgtcccg tggatagaaa aaaggaaatg ctatgaagtc tgtattaaaa
    10201 gtttcactgg ctgcactgac cctggctttt gcggtttctt ctcatgccgc ggataaaaaa
    10261 ttagttgtcg cgacggatac caccttcgtt ccgtttgaat ttaaacaggg cgataaatac
    10321 gtgggctttg acgttgatct gtgggctgcc atcgctaaag agctgaagct ggattacgaa
    10381 ctgaagccga tggatttcag tgggatcatt ccggcactgc aaaccaaaaa cgtcgatctg
    10441 gcgctggcgg gcatcaccat taccgacgag cgtaaaaaag cgatcgattt ctctgacggc
    10501 tactacaaaa gcggcctgtt agtgatggtg aaagccaaca ataacgatgt gaaaagcgtg
    10561 aaagatctcg acgggaaagt ggttgctgtg aagagcggca ctggctccgt tgattacgcg
    10621 aaagcaaaca tcaaaactaa agatctgcgt cagttcccga acatcgataa cgcctatatg
    10681 gaactgggca ccaaccgcgc agacgccgtt ctgcacgata cgccaaacat tctgtacttc
    10741 atcaaaaccg ccggtaacgg tcagttcaaa gcggtaggtg actctctgga agcacagcaa
    10801 tacggtattg cgttcccgaa aggtagcgac gagctgcgtg acaaagtcaa cggcgcgttg
    10861 aaaaccctgc gcgagaacgg aacttacaac gaaatctaca aaaaatggtt cggtactgaa
    10921 ccgaaataat aacgctacgc ctgtaaggcg cactggcagt tccctctccc ctctggggag
    10981 gatctattga tatcaactga tttcaacaga gctaacccct ccccctttaa ccccctttga
    11041 aataagatat catcagaaat tccttttctc tgggggtcaa tcatattcca tgaacatcaa
    11101 gatgattttg ggggctattc tgg
//
LOCUS       DAMZFT010000152        11062 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.152, whole genome shotgun sequence.
ACCESSION   DAMZFT010000152 DAMZFT010000000
VERSION     DAMZFT010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11062)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11062)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11062
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.152"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..587
                     /locus_tag="PN977_004249"
     CDS             <1..587
                     /locus_tag="PN977_004249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDI6044215.1"
                     /translation="AGKSTLLSLITGDHPQGYSNDLTLFGRRRGSGETIWDIKKHIGY
                     VSSSLHLDYRVSTTVRNVILSGYFDSIGIYQAVSDRQQKLVQQWLDILGIDKRTADAP
                     FHSLSWGQQRLALIVRALVKHPTLLILDEPLQGLDPLNRQLIRRFVDVLISEGETQLL
                     FVSHHAEDAPACITHRLEFVPDGDFYRYALTKIN"
     gene            849..1865
                     /gene="galE"
                     /locus_tag="PN977_004250"
     CDS             849..1865
                     /gene="galE"
                     /locus_tag="PN977_004250"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308814.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HDI6044216.1"
                     /translation="MRVLVTGGSGYIGSHTCVQLLQNGHDVIILDNLCNSKRSVLPVI
                     ERLGGKHPTFVEGDIRNEALMTEILHDHAIDTVIHFAGLKAVGESVQKPLEYYDNNVN
                     GTLRLISAMRAANVKNFIFSSSATVYGDQPKIPYVESFPTGTPQSPYGKSKLMVEQIL
                     TDLQKAQPDWSIALLRYFNPVGAHPSGDMGEDPQGIPNNLMPYIAQVAVGRRDSLAIF
                     GNDYPTEDGTGVRDYIHVMDLADGHVVAMEKLANKPGVHIYNLGAGVGSSVLDVVNAF
                     SKACGKPVNYHFAPRREGDLPAYWADASKADRELNWRVTRTLDEMAQDTWHWQSRHPQ
                     GYPD"
     gene            1875..2921
                     /gene="galT"
                     /locus_tag="PN977_004251"
     CDS             1875..2921
                     /gene="galT"
                     /locus_tag="PN977_004251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HDI6044217.1"
                     /translation="MTQFNPVDHPHRRYNPLTGQWILVSPHRAKRPWQGAQETPAKQV
                     LPAHDPDCFLCAGNVRVTGDKNPDYTGTYVFTNDFAALMSDTPDAPESNDPLMRCQSA
                     RGTSRVICFSPDHSKTLPELSVAALTEIVKTWQEQTAELGKTYPWVQVFENKGAAMGC
                     SNPHPHGQIWANSFLPNEAEREDRLQKEYFAGQKSPMLVDYVQRELADGSRTVVETEH
                     WLAVVPYWAAWPFETLLLPKAHVLWITDLTDAQRSDLALALKKLTSRYDNLFQCSFPY
                     SMGWHGAPFNGEENQHWQLHAHFYPPLLRSATVRKFMVGYEMLAETQRDLTAEQAAER
                     LRAVSDIHFRESGV"
     gene            2925..4073
                     /gene="galK"
                     /locus_tag="PN977_004252"
     CDS             2925..4073
                     /gene="galK"
                     /locus_tag="PN977_004252"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001713229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HDI6044218.1"
                     /translation="MSLKEKTQSLFANAFGYPATHTIQAPGRVNLIGEHTDYNDGFVL
                     PCAIDYQTVISCAPRDDRKVRVMAADYENQLDEFSLDAPIVAHESYQWANYVRGVVKH
                     LQLRNNNFGGVDMVISGNVPQGAGLSSSASLEVAVGTVLQQLYHLPLDGAQIALNGQE
                     AENQFVGCNCGIMDQLISALGKKDHALLIDCRSLGTKAVSMPKGVAVVIINSNFKRTL
                     VGSEYNTRREQCETGARFFQQPALRDVTIEEFNAVAHELDPIVAKRVRHILTENARTV
                     EAASALEQGDLKRMGELMAESHASMRDDFEITVPQIDTLVEIVKAVIGDKGGVRMTGG
                     GFGGCIVALIPEELVPAVQQAVAEQYEAKTGIKETFYVCKPSQGAGQC"
     gene            4067..5107
                     /gene="galM"
                     /locus_tag="PN977_004253"
     CDS             4067..5107
                     /gene="galM"
                     /locus_tag="PN977_004253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-epimerase"
                     /protein_id="HDI6044219.1"
                     /translation="MLNETPALAPDGQPYRLLTLRNNAGMVVTLMDWGATLLSARIPL
                     SDGSVREALLGCASPECYQDQAAFLGASIGRYANRIANSRYTFDGETVTLSPSQGVNQ
                     LHGGPEGFDKRRWQIVNQNDRQVLFALSSDDGDQGFPGNLGATVQYRLTDDNRISITY
                     RATVDKPCPVNMTNHVYFNLDGEQSDVRNHKLQILADEYLPVDEGGIPHDGLKSVAGT
                     SFDFRSAKIIASEFLADDDQRKVKGYDHAFLLQAKGDGKKVAAHVWSADEKLQLKVYT
                     TAPALQFYSGNFLGGTPSRGTEPYADWQGLALESEFLPDSPNHPEWPQPDCFLRPGEE
                     YSSLTEYQFIAE"
     gene            5310..6062
                     /gene="gpmA"
                     /locus_tag="PN977_004254"
     CDS             5310..6062
                     /gene="gpmA"
                     /locus_tag="PN977_004254"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000301556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HDI6044220.1"
                     /translation="MAVTKLVLVRHGESQWNKENRFTGWYDVDLSEKGVSEAKAAGKL
                     LKEEGYSFDFAYTSVLKRAIHTLWNVLDELDQAWLPVEKSWKLNERHYGALQGLNKAE
                     TAEKYGDEQVKQWRRGFAVTPPELTKDDERYPGHDPRYAKLSEKELPLTESLALTIDR
                     VIPYWNETILPRMKSGERVIIAAHGNSLRALVKYLDNMSEEEILELNIPTGVPLVYEF
                     DENFKPLKRYYLGNADEIAAKAAAVANQGKAK"
     gene            complement(6210..7262)
                     /gene="aroG"
                     /locus_tag="PN977_004255"
     CDS             complement(6210..7262)
                     /gene="aroG"
                     /locus_tag="PN977_004255"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroG"
                     /protein_id="HDI6044221.1"
                     /translation="MNYQNDDLRIKEIKELLPPVALLEKFPATENAANTVAHARKAIH
                     KILKGNDDRLLVVIGPCSIHDPVAAKEYATRLLALREELKDELEIVMRVYFEKPRTTV
                     GWKGLINDPHMDNSFQINDGLRIARKLLLDINDSGLPAAGEFLDMITPQYLADLMSWG
                     AIGARTTESQVHRELASGLSCPVGFKNGTDGTIKVAIDAINAAGAPHCFLSVTKWGHS
                     AIVNTSGNGDCHIILRGGKEPNYSAKHVAEVKEGLNKAGLPAQVMIDFSHANSSKQFK
                     KQMDVCADVCQQIAGGEKAIIGVMVESHLVEGNQSLESGEPLAYGKSITDACIGWDDT
                     DALLRQLANAVKARRG"
     gene            7578..7958
                     /locus_tag="PN977_004256"
     CDS             7578..7958
                     /locus_tag="PN977_004256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgS-like family protein"
                     /protein_id="HDI6044222.1"
                     /translation="MKMTKLATLFLTATLSLASGAALAADSGAQTNNGQANAAADAGQ
                     VAPDARENVAPNNVDNNGVNTGSGGTMLHSDGSSMNNDGMTKDEEHKNTMCKDGRCPD
                     INKKVQTGDGINNDVDTKTDGTTQ"
     gene            8072..9013
                     /gene="zitB"
                     /locus_tag="PN977_004257"
                     /pseudo
     CDS             8072..9013
                     /gene="zitB"
                     /locus_tag="PN977_004257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308807.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family zinc transporter ZitB"
     gene            complement(9010..9729)
                     /gene="pnuC"
                     /locus_tag="PN977_004258"
     CDS             complement(9010..9729)
                     /gene="pnuC"
                     /locus_tag="PN977_004258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HDI6044223.1"
                     /translation="MDFFSVQNILVHIPIGAGGYDLSWIEAVGTIAGLLCIGLASLEK
                     ISNYFFGLINVTLFGIIFFQIQLYASLLLQMFFFAANIYGWHAWSRQTSQNEAELKIR
                     WLPLPKALSWLAVCVVSIGLMTVFINPVFAFLTRVAVMIMQALGLQVVMPELQPDAFP
                     FWDSCMMVLSIVAMILMTRKYVENWLLWVIINVISVVIFALQGVYAMSLEYIILTFIA
                     LNGSRMWINSARERGSRALSH"
     gene            complement(9767..10810)
                     /gene="nadA"
                     /locus_tag="PN977_004259"
     CDS             complement(9767..10810)
                     /gene="nadA"
                     /locus_tag="PN977_004259"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HDI6044224.1"
                     /translation="MSVMFDPDTAIYPFPPKPTPLSIDEKAYYREKIKRLLKERNAVM
                     VAHYYTDPEIQQLAEETGGCISDSLEMARFGAKHPASTLLVAGVRFMGETAKILSPEK
                     TILMPTLQAECSLDLGCPVEEFNAFCDAHPDRTVVVYANTSAAVKARADWVVTSSIAV
                     ELIDHLDSLGEKIIWAPDKHLGRYVQKQTGADILCWQGACIVHDEFKTQALTRLQEEY
                     PDAAILVHPESPQAIVDMADAVGSTSQLIAAAKTLPHQRLIVATDRGIFYKMQQAVPD
                     KELLEAPTAGEGATCRSCAHCPWMAMNGLQAIAEALEQEGSNHEVHVDERLRERALVP
                     LNRMLEFAATLRG"
BASE COUNT         2749 a         2875 c         2779 g         2659 t
ORIGIN      
        1 gcgcgggaaa atcgacgtta ttaagcctga ttactggcga tcatccgcaa ggttacagca
       61 acgatttgac gcttttcgga cgacgtcgcg gcagcggcga aaccatctgg gatatcaaaa
      121 agcatatcgg ttacgtcagc agtagtttgc atctggatta ccgggtcagc actactgtgc
      181 gtaatgtgat cctttctggc tattttgatt cgattggcat ttatcaggcc gtatcggatc
      241 gccagcaaaa actggtgcag cagtggctgg atattctcgg cattgataaa cgcacggctg
      301 acgctccttt ccatagtctt tcctggggac agcagcgtct ggcgctgatc gtccgtgcgc
      361 tagtgaaaca tccgacattg cttattctcg atgaaccact acaggggctt gatccgctca
      421 atcgccagct tatccgccgt tttgttgatg tgctgattag cgaaggtgaa acgcaattgt
      481 tgtttgtttc gcaccacgct gaagatgcgc ctgcctgtat tacccatcgt ctggagttcg
      541 tgccggacgg tgacttttat cgctatgcgc tgacaaaaat aaactgagtc ggtagtgctg
      601 accgtgccgg aggcggcgta aacgtgttat ccggccaacg gtttgacgca tgcaggcatg
      661 aaaccgcgtc tttttttcag ataaaaagtg caatcattca taaaccctct gttttataat
      721 cacttaatcg cgcataaaaa acggctaaat tcttgtgtaa acgattccac taatttattc
      781 catgtcacac ttttcgcatc tttgttatgc tatggttatt tcataccata agcctaatgg
      841 agcgaattat gagagttctg gttaccggtg gtagcggtta cattggaagt catacctgtg
      901 tgcaattact gcaaaacggt catgatgtca tcattcttga taacctctgt aacagtaagc
      961 gcagcgtact gcctgttatc gagcgtttag gcggcaaaca tccaacgttt gttgaaggcg
     1021 atatccgtaa cgaagcgttg atgaccgaga tcctgcacga tcacgctatc gacaccgtga
     1081 tccacttcgc cgggctgaaa gccgttggcg aatcggtaca aaaaccgctt gaatattacg
     1141 acaacaatgt caacggtact ctgcgcctga ttagcgccat gcgcgccgct aacgtcaaaa
     1201 actttatctt tagctcctcc gccaccgttt atggcgatca gcccaaaatt ccatatgttg
     1261 aaagcttccc gaccggcaca ccgcaaagcc cttacggcaa aagcaagctg atggtggaac
     1321 agatcctcac cgatctgcaa aaagcccagc cggactggag cattgccctg ctgcgctact
     1381 tcaacccagt tggcgcgcat ccgtcgggcg atatgggcga agatccgcaa ggcattccga
     1441 ataacctgat gccatacatc gcccaggttg ctgtaggccg tcgcgactcg ctggcgattt
     1501 ttggtaacga ttatccgacc gaagacggta ctggcgtgcg cgattacatc cacgtaatgg
     1561 atctggcgga cggtcacgtc gtagcgatgg aaaaactggc gaacaagcca ggcgtacaca
     1621 tctacaacct cggcgctggc gtgggcagca gcgtgctgga cgtggttaat gccttcagca
     1681 aagcctgcgg caaaccggtt aattatcatt ttgcaccgcg tcgcgagggc gaccttccgg
     1741 cctactgggc ggacgccagc aaagccgacc gtgaactgaa ctggcgcgta acgcgcacac
     1801 tcgatgaaat ggcgcaggac acctggcact ggcagtcacg ccatccacag ggatatcccg
     1861 attaaggaac gaccatgacg caatttaatc ccgttgatca tccacatcgc cgctacaacc
     1921 cgctcaccgg gcaatggatt ctggtttcac cgcaccgcgc taagcgcccc tggcaggggg
     1981 cgcaggaaac gccagccaaa caggtgttac ctgcgcacga tccagattgc ttcctctgcg
     2041 caggtaatgt gcgggtgaca ggcgataaaa accccgatta caccgggact tacgttttca
     2101 ctaatgactt tgcggccttg atgtctgaca cgccagatgc gccagaaagc aacgatccgc
     2161 taatgcgttg ccagagcgcg cgcggtacca gccgggtgat ctgcttttca ccggatcaca
     2221 gtaaaacgtt gccagaactg agcgttgcgg cattgacgga aatcgtcaaa acctggcagg
     2281 agcaaaccgc agagctggga aaaacgtacc cgtgggtaca ggtctttgaa aacaaaggcg
     2341 cggcgatggg ctgctctaac ccgcatccgc acggacagat ttgggcaaat agcttcctgc
     2401 ctaacgaagc tgagcgcgaa gaccgcctgc aaaaagaata tttcgccggg cagaaatcac
     2461 caatgctggt ggattatgtt cagcgcgagc tggcagacgg tagccgtacc gttgtcgaaa
     2521 ccgaacactg gttagccgtt gtaccttact gggctgcctg gccgttcgaa acgctactgc
     2581 tgcccaaagc ccacgttttg tggatcaccg atttgaccga cgcccagcgc agcgatttgg
     2641 cactggcgtt gaaaaagctg accagtcgtt atgacaacct cttccagtgc tccttcccct
     2701 actctatggg ctggcacggc gcgccattta atggcgaaga gaatcaacac tggcagctgc
     2761 acgcgcactt ttatccgcct ctgttgcgct ccgccaccgt acgtaaattt atggttggtt
     2821 atgaaatgct ggcagaaacc cagcgagacc tgaccgcaga acaggcagca gagcgtttgc
     2881 gcgcagtcag cgatatccat tttcgcgaat ccggagtgta agaaatgagt ctgaaagaaa
     2941 aaacacaatc tctgtttgcc aacgcatttg gctaccctgc cactcacacc attcaggcgc
     3001 ctggccgcgt gaatttgatt ggtgaacaca ccgactacaa cgacggtttc gttctgccct
     3061 gcgcgattga ttatcaaacc gtgatcagtt gtgcaccacg cgatgaccgt aaagttcgcg
     3121 tgatggcagc cgattatgaa aatcagctcg atgagttttc cctcgatgcg cccattgtcg
     3181 cacatgaaag ctatcaatgg gctaactacg ttcgtggcgt ggtgaaacat ctgcaactgc
     3241 gtaacaacaa cttcggcggt gtggacatgg tgatcagcgg caatgtgccg cagggtgccg
     3301 ggttaagttc ttccgcttca ctggaagtcg cggtcggaac cgtattgcag cagctttatc
     3361 atctgccgct ggacggcgca caaatcgcgc ttaatggtca ggaagcagaa aaccagtttg
     3421 taggctgtaa ctgtggaatc atggatcaac tgatttccgc gctcggcaag aaagatcatg
     3481 ccttgctgat cgattgccgc tcactgggga ccaaagctgt ttccatgccg aaaggcgtgg
     3541 ctgtcgtcat catcaacagt aacttcaaac gtaccctggt tggcagcgaa tacaacaccc
     3601 gtcgtgaaca gtgcgaaacc ggtgcgcgtt tcttccagca gccagcgctg cgcgatgtca
     3661 ccattgaaga gttcaacgct gttgcgcatg aactggaccc aatcgtggcg aaacgcgtgc
     3721 gtcatatcct gactgaaaac gcccgcaccg ttgaagctgc cagcgcgctg gagcaaggcg
     3781 acctgaaacg tatgggcgag ttgatggcgg agtctcatgc ctctatgcgc gatgatttcg
     3841 aaatcaccgt gccgcaaatt gacactctgg tagaaatcgt caaagctgtg attggcgaca
     3901 aaggtggcgt acgcatgacc ggcggcggat ttggcggctg catcgtcgcg ctgatcccgg
     3961 aagagctggt gcctgcagta cagcaagctg tcgctgaaca atatgaagca aaaacaggta
     4021 ttaaagagac tttttacgtt tgtaaaccat cacaaggagc aggacagtgc tgaacgaaac
     4081 tcccgcactg gcacccgatg gtcagccgta ccgactgtta actttgcgta acaacgcagg
     4141 gatggtagtc acgctgatgg actggggtgc gactttactt tccgcccgta ttccgctttc
     4201 cgatggcagc gtccgcgagg cgctgcttgg ctgcgccagc ccggaatgct atcaggatca
     4261 ggccgcattt ctgggggcct ctattggtcg ttatgccaac cgcatcgcca atagccgtta
     4321 tacctttgac ggtgaaaccg tgacgctttc gccaagtcag ggcgttaacc agctgcacgg
     4381 cggcccggaa gggttcgaca aacgtcgctg gcagattgtg aaccagaatg atcgtcaggt
     4441 gctatttgcc ctgagttcag atgatggcga tcagggcttc ccgggtaatc tcggcgcgac
     4501 ggtgcaatat cgtctgaccg acgataaccg tatctccatt acttatcgcg ccacagttga
     4561 taaaccttgc ccggtgaata tgactaatca cgtctatttc aacctcgacg gcgagcagtc
     4621 tgacgtgcgc aatcacaagt tgcagattct ggcggacgaa tatctgccgg ttgatgaagg
     4681 cggcattccg cacgacggtc tgaaatctgt cgccggaaca tcttttgatt tccgcagcgc
     4741 caaaatcatc gccagtgagt ttcttgccga cgacgatcag cgcaaagtga aaggttacga
     4801 tcacgcgttc ttgttacagg ccaaaggcga tggcaagaaa gtggcggcgc atgtctggtc
     4861 agcagatgaa aaattgcagc tgaaggtcta caccaccgct ccggctctgc aattctactc
     4921 cggcaacttc ctcggcggca caccgtcgcg gggaaccgaa ccttacgccg actggcaagg
     4981 gctggctctg gaaagcgaat ttctgccgga cagtccgaac caccctgaat ggccgcaacc
     5041 ggactgcttc ctgcgtcctg gcgaagagta ttccagcctg acggaatatc agtttattgc
     5101 tgagtaatgt taagccctcg tcatgagggc tttatcttca tattgttcaa atcaccagca
     5161 aacaccgaca tatttgcaac tcaatattca caacaacctt acactgcgac actattttcg
     5221 ctatggttat gcgtaagcat tgctgttgct tcgtcacggc aatataatga gaattattat
     5281 cattaaaaga tgatttgagg agtaagtata tggctgtaac taagctggtt ctggttcgtc
     5341 atggcgaaag tcagtggaac aaagaaaacc gtttcaccgg ttggtacgac gtggatctgt
     5401 ctgagaaagg cgtaagcgaa gcaaaagcag caggtaagct gctgaaagag gaaggttaca
     5461 gctttgactt tgcttacacc tctgtgctga aacgcgctat tcataccctg tggaatgtgc
     5521 tggacgaact ggatcaggca tggctgcccg ttgagaaatc ctggaaactg aacgaacgtc
     5581 actacggtgc attgcagggt ctgaacaaag cggaaaccgc tgaaaagtat ggcgacgagc
     5641 aggtgaaaca gtggcgtcgt ggttttgcgg tgactccgcc ggaactgact aaagatgatg
     5701 agcgttatcc gggtcacgat ccgcgttacg cgaaactgag cgagaaagag ctgccactga
     5761 cggaaagcct ggcgctgacc attgaccgcg tgatccctta ctggaatgaa accattctgc
     5821 cgcgtatgaa gagcggtgag cgcgtcatca tcgctgcaca cggtaactcg ttacgtgcgc
     5881 tggtgaaata tctcgataac atgagcgaag aagagattct tgagctgaat atcccgaccg
     5941 gcgtgccgct ggtttatgag tttgacgaga acttcaaacc gctgaaacgc tattatctgg
     6001 gtaatgctga tgagatcgcg gcgaaagcag cagccgttgc gaaccagggt aaagcgaagt
     6061 aaacgtcatt cgtttaaaaa tgagaaagcc gactgcaagt gagtcggctt ttttgtttgt
     6121 cgttgctaac aatggagcac attgcctgat gcgacgctgc gcgtcttatc agggctgcaa
     6181 acgtacagtc atagcgattc gactataaat tacccgcgac gcgcttttac tgcattcgcc
     6241 agttgacgta acagagcatc ggtatcatcc cagccaatgc aggcatcggt gatgctcttg
     6301 ccataagcca gcggttcccc gctctccagg ctctgattgc cttccaccag atggctttcc
     6361 accatcacgc caataatagc cttttcgcca ccggcaatct gctggcaaac gtcagcacaa
     6421 acatccatct gctttttgaa ttgttttgac gagttagcat ggctgaaatc gatcatcacc
     6481 tgtgctggca ggcctgcttt gttcagccct tctttcactt cagcaacgtg cttcgcgctg
     6541 tagttaggct ctttaccgcc gcgcagaatg atatggcaat cgccgttacc gctggtattc
     6601 acaatcgccg aatgccccca tttcgttacg gacaggaagc agtgcggcgc accggcggca
     6661 ttaatagcat cgatagccac tttaatcgta ccatcagtgc catttttgaa gcctaccggg
     6721 caagaaagac cagacgccag ttcgcggtgc acctgcgatt ccgtagtacg tgcgccaatt
     6781 gcgccccagc tcatcaggtc agcgagatat tgtggggtga tcatatccag gaattcaccc
     6841 gccgctggca gaccgctgtc gttaatatcg agcagcaatt tgcgggcaat acgcagaccg
     6901 tcgttgatct ggaagctgtt atccatatgc ggatcgttaa tcagcccttt ccagcccacc
     6961 gttgtacgcg gcttttcaaa atagacgcgc attacgattt ccagctcatc tttcagctct
     7021 tcacgcagcg ccagcaagcg agtggcatac tcttttgctg cgacaggatc atggattgag
     7081 catgggccaa tcaccaccaa caggcgatca tcattacctt tcaggatctt atggatcgct
     7141 tttcgggcat gggcgaccgt attcgcggca ttttcagtag cggggaattt ttccagcaat
     7201 gcgacaggag gaagtaactc tttgatttct ttgatgcgta aatcgtcgtt ctgataattc
     7261 atgtctgttc cagtgttgcc atacttatct tagtgaatgc aatacttcca atctatatct
     7321 tccgtcagaa tgtgtaaacg gggttttaca ctatgaacgg attaatcctg gaattagcaa
     7381 gaaaaacgcc agattgtcgc gaaaaacgag atctctccta caatttctaa ctgtaactcc
     7441 tttgtttgtt aattatttca agattctctg ctgggtatct ttgcctggct gaaatcttaa
     7501 accattgcct tatattcacc tgcaaatgca ctgttggaag aggttatccg acataacgac
     7561 cataacagga gcatcctatg aaaatgacaa aactggccac actatttttg actgccactc
     7621 taagccttgc cagcggtgcc gcactggctg ccgatagcgg agcacaaact aataacggcc
     7681 aggcaaacgc cgcagctgat gcgggccagg tagcccctga cgcccgtgaa aatgtcgcgc
     7741 caaataacgt cgacaataac ggggtaaata ccggttctgg tggcacaatg ctgcattcgg
     7801 atggttcttc aatgaacaat gacggaatga ccaaagatga agagcacaag aacaccatgt
     7861 gcaaagatgg ccgctgtcct gatatcaata aaaaagtcca aaccggtgat ggcatcaaca
     7921 atgatgtcga taccaaaacc gacggtacca cgcagtaacc ctgcgaaata gccaataatg
     7981 ggagagcgga gtctctccct tttaattttt cccttctaaa agcagtatga tgtcataaag
     8041 ttaagtgaga ataattaagg gatgactcgt catggcgcac tcacactcac acacttcctc
     8101 acacctgcca gaagataata atgctcgtcg cttgttgtat gctttcggcg tgactgccgg
     8161 gtttatgctc gtagaagtca ttggtggttt tctttccggt tctctggcat tgctggccga
     8221 tgcgggtcat atgttgaccg atactgccgc cctgcttttc gccctactcg cagtgcaatt
     8281 ttcccgtcgt cctcccacca ttcgccacac tttcggttgg ctaagattga ccacgctcgc
     8341 ggcgtttgtg aatgccatcg ccctggtggt gattactatt ttgattgtct gggaggcgat
     8401 agaacgcttc cgcactccgc gtccggtcga gggcggcatg atgatggcaa ttgccgtggc
     8461 tgggctgctg gcaaatatac tttctttctg gttacttcat cacggcagct aagagaaaaa
     8521 cctcaacgtg cgagcagcgg cactgcatgt gctcggggac ctgctgggtt cggttggggc
     8581 tattattgcc gcgttgatta ttatctggac cggctggaca cctgctgacc ccattctctc
     8641 aatactggtg tcgcttctgg ttctgcgtag cgcgtggcga ctgttgaaag atagcgtgaa
     8701 tgaattactt gaaggtgcac cggtatcgct ggatatcgcc gaactgaagc gtcgtatgtg
     8761 ccgggaaatc ccggaagtcc gcaatgtgca ccatgtacat gtatggatgg taggcgagaa
     8821 gccggtgatg acgctgcatg tgcaggtgat cccgccacac gatcacgatg ccttgttaga
     8881 tcagatccaa cactacctga tggatcacta tcagattgag cacgccacta ttcagatgga
     8941 atatcaacct tgtcatgggc cagactgcca cctgaatgaa ggggtgtctg gccattcaca
     9001 tcatcaccat taatgggaca gcgcgcgtga acctctttcg cgtgcgctgt tgatccacat
     9061 ccggctgccg ttgagggcaa taaaggtcag gatgatgtac tccagagaca tggcgtaaac
     9121 gccctgaagt gcaaaaataa cgacgctaat cacgttaata atcacccaca acagccagtt
     9181 ttccacatac ttacgcgtca tcagaatcat tgccacgata gataacacca tcatgcatga
     9241 atcccagaac gggaaagcgt ccggttgcag ttcaggcatc acaacctgta atcctaatgc
     9301 ttgcatgatc atgactgcca cgcgggtcaa aaatgcaaac accggattga taaataccgt
     9361 catcagacca atcgaaacaa cgcaaaccgc caaccagctt agcgccttcg gcaatggcaa
     9421 ccagcgaatt ttcaactccg cctcgttctg actggtttgt cgcgaccacg catgccagcc
     9481 gtaaatattc gcggcaaaga aaaacatctg taatagcagg ctggcataca gctgaatctg
     9541 aaagaaaata atgccaaaca aggtgacgtt gatcaggcca aaaaagtagt tgctgatctt
     9601 ctccagactg gcaaggccaa tacacagcaa cccggcgatt gtgcctaccg cttcgatcca
     9661 tgagagatca taaccgcctg cccctattgg tatatgtacc aggatattct gcacactaaa
     9721 aaaatccatc tttcccccaa agcgtaacgt tacgccttat tattcgttat ccacgtagtg
     9781 tagccgcaaa ttccagcata cgattgagcg gcaccagcgc cctctctcgc agcctttcat
     9841 caacatgaac ctcgtgattg cttccttcct gttctaatgc ctctgcgata gcctgaaggc
     9901 cattcatggc catccacgga caatgcgcgc agctgcggca ggttgcaccc tcacccgcgg
     9961 ttggtgcttc cagtaactct ttatctggca ccgcctgctg cattttgtag aaaatgcccc
    10021 gatcggttgc cacgataagt cgctgatgtg gcaatgtttt cgcagcagcg atcagttgac
    10081 tggtggaacc gaccgcatcc gccatatcga caatagcttg cggtgattct ggatgcacca
    10141 gtatggcagc atccgggtat tcttcttgca agcgggttaa cgcctgagtc ttaaattcat
    10201 catgcacaat gcaggcaccc tgccagcata aaatgtctgc gcccgtctgt ttttgcacgt
    10261 aacgccccag atgtttgtcg ggtgcccaga tgattttttc acccaaacta tccagatgat
    10321 caataagttc gacggcaatg cttgaagtta ccacccaatc tgcgcgcgct tttaccgcag
    10381 cagaagtgtt ggcgtagacg acgacagtac gatcgggatg ggcatcgcaa aatgcgttaa
    10441 attcttcaac agggcagccg agatccagtg aacattcagc ctgaagtgtc ggcatcagaa
    10501 ttgttttttc cggactgaga attttggcgg tttctcccat aaatctcacc ccagcgacta
    10561 acaacgtaga agcgggatgc tttgcaccga agcgcgccat ttccagagaa tcagaaatac
    10621 agccaccggt ttcttctgcc agttgttgaa tttcgggatc ggtatagtag tgggcaacca
    10681 tcaccgcatt acgttctttt agcagacgtt ttatcttctc gcggtaatac gctttttcat
    10741 caatgcttaa cggcgtcggc ttcgggggga aaggataaat cgccgtgtct ggatcaaaca
    10801 tcacgctcat cttaccatct cgttttacag gcttaacgtt aaaaccgaca tgagcgtaat
    10861 attcgctgtt atcggtgaaa tttgttttat atgctaaaca agatagtcga atggtgggga
    10921 aaagtcactc gaattacgcc taattccacc gacgtagagt tgcttgcaca gtaagctctg
    10981 acgatgtcat caggtttaca aagtgaaatt gaggatacct gaatagcaaa aaagcgcctt
    11041 tagggcgctt ttttacattg gt
//
LOCUS       DAMZFT010000153        10990 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.153, whole genome shotgun sequence.
ACCESSION   DAMZFT010000153 DAMZFT010000000
VERSION     DAMZFT010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10990)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10990)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10990
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.153"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(47..586)
                     /locus_tag="PN977_004260"
     CDS             complement(47..586)
                     /locus_tag="PN977_004260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2058 domain-containing protein"
                     /protein_id="HDI6044225.1"
                     /translation="MAKLTLQEQLLKAGLVTSKKAAKVERTAKKSRVQAREARAAVEE
                     NKKAQLERDKQLSEQQKQAALAKEYKAQVKQLIEMNRITIANGDIGFNFTDGNLIKKI
                     FVDKLTQTQLINGRLAIARLLVDNNSEGEYAIIPASVADKIAQRDASSIALHSALSAE
                     EQDEDDPYADFKVPDDLMW"
     gene            complement(688..1899)
                     /gene="mhpT"
                     /locus_tag="PN977_004261"
     CDS             complement(688..1899)
                     /gene="mhpT"
                     /locus_tag="PN977_004261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-(3-hydroxy-phenyl)propionate transporter MhpT"
                     /protein_id="HDI6044226.1"
                     /translation="MSTRTPSSSSSRLMLTIGLCFLVALMEGLDLQAAGIAAGGIAQA
                     FALDKMQMGWIFSAGILGLLPGALVGGMLADRYGRKRILIGSVALFGLFSLATAIAWD
                     FPSLVFARLMTGVGLGAALPNLIALTSEAAGPRFRGTAVSLMYCGVPIGAALAATLGF
                     AGANLAWQTVFWVGGVVPLILVPLLMRWLPESAVFAGEKQAAPPLRALFAPETATATL
                     LLWLCYFFTLLVVYMLINWLPLLLVEQGFQPSQAAGVMFALQMGAASGTLMLGALMDK
                     LRPVTMSLLIYSGMLASLLALGTVSSFNGMLLAGFVAGLFATGGQSVLYALAPLFYSS
                     QIRATGVGTAVAVGRLGAMSGPLLAGKMLALGTGTVGVMAASAPGILVAGLAVFILMS
                     RRSRMQPCADA"
     gene            complement(2075..3088)
                     /gene="dmpG"
                     /locus_tag="PN977_004262"
     CDS             complement(2075..3088)
                     /gene="dmpG"
                     /locus_tag="PN977_004262"
                     /EC_number="4.1.3.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-2-oxovalerate aldolase"
                     /protein_id="HDI6044227.1"
                     /translation="MNDKKLYISDVTLRDGMHAIRHQYSLENVRQVAKALDDARVDSI
                     EVAHGDGLQGSSFNYGFGAHSDLEWIEAAADVVKHAKIATLLLPGIGTIHDLKNAWQA
                     GARVVRVATHCTEADVSAQHIQYARELGMDTVGFLMMSHMTTPENLAKQAKLMEGYGA
                     TCIYVVDSGGAMNMSDIRDRFRALKAVLKPETQTGIHAHHNLSLGVANSIAAVEEGCD
                     RIDASLAGMGAGAGNAPLEVFIAAADKLGWQHGTDLYALMDAADDLVRPLQDRPVRVD
                     RETLALGYAGVYSSFLRHCETAAARYGLSAVDILVELGKRRMVGGQEDMIVDVALDLR
                     NNK"
     gene            complement(3085..4035)
                     /gene="mhpF"
                     /locus_tag="PN977_004263"
     CDS             complement(3085..4035)
                     /gene="mhpF"
                     /locus_tag="PN977_004263"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetaldehyde dehydrogenase"
                     /protein_id="HDI6044228.1"
                     /translation="MSKRKVAIIGSGNIGTDLMIKILRHGQHLEMAVMVGIDPQSDGL
                     ARARRMGVATTHEGVIGLMNMPEFADIDIVFDATSAGAHVKNDAALREAKPDIRLIDL
                     TPAAIGPYCVPVVNLEANVDQLNVNMVTCGGQATIPMVAAVSRVARVHYAEIIASIAS
                     KSAGPGTRANIDEFTETTSRAIEVVGGAAKGKAIIVLNPAEPPLMMRDTVYVLSDEAS
                     QDDIEASINEMAEAVQAYVPGYRLKQRVQFEVIPQDKPVNLPGVGQFSGLKTAVWLEV
                     EGAAHYLPAYAGNLDIMTSSALATAEKMAQSLARKAGEAA"
     gene            complement(4032..4841)
                     /gene="mhpD"
                     /locus_tag="PN977_004264"
     CDS             complement(4032..4841)
                     /gene="mhpD"
                     /locus_tag="PN977_004264"
                     /EC_number="4.2.1.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-4-pentenoate hydratase"
                     /protein_id="HDI6044229.1"
                     /translation="MTKHTLEQLAADLRRAAEQGEAIAPLRDLIGIDNAEAAYAIQHI
                     NVQHDVAQGRRVVGRKVGLTHPKVQQQLGVDQPDFGTLFADMCYGDNETIPFSRVLQP
                     RIEAEIALVLNRDLPATDITFDELYNAIEWVLPALEVVGSRIRDWSIQFVDTVADNAS
                     CGVYVIGGPAQRPAGLDLKNCAMKMTRNNEEVSSGRGSECLGHPLNAAVWLARKMASL
                     GEPLRAGDIILTGALGPMVAVNAGDRFEAHIEGIGSVAATFSSAAPKGSLS"
     gene            complement(4851..5717)
                     /gene="mhpC"
                     /locus_tag="PN977_004265"
     CDS             complement(4851..5717)
                     /gene="mhpC"
                     /locus_tag="PN977_004265"
                     /EC_number="3.7.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414883.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-6-oxonona-2,4-dienedioate hydrolase"
                     /protein_id="HDI6044230.1"
                     /translation="MSYQPQTEAATSRFLNVEEAGKTLRIHFNDCGQGDETVVLLHGS
                     GPGATGWANFSRNIDPLVEAGYRVILLDCPGWGKSDSIVNSGSRSDLNARILKSVVDQ
                     LDIAKIHLLGNSMGGHSSVAFTLNWPERVGKLVLMGGGTGGMSLFTPMPTEGIKRLNQ
                     LYRQPTIENLKLMMDIFVFDTSDLTDALFEARLNNMLSRRDHLENFVKSLEANPKQFP
                     DFGPRLAEIKAQTLIVWGRNDRFVPMDAGLRLLSGIAGSELHIFRDCGHWAQWEHADA
                     FNQLVLNFLARP"
     gene            complement(5735..6679)
                     /gene="mhpB"
                     /locus_tag="PN977_004266"
     CDS             complement(5735..6679)
                     /gene="mhpB"
                     /locus_tag="PN977_004266"
                     /EC_number="1.13.11.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-carboxyethylcatechol 2,3-dioxygenase"
                     /protein_id="HDI6044231.1"
                     /translation="MHAYLHCLSHSPLVGYVDPAQEVLDEVNGVIASARERIAAFSPE
                     LVVLFAPDHYNGFFYDVMPPFCLGVGATAIGDFGSAAGELPVPVELAEACAHAVMKSG
                     IDLAVSYCMQVDHGFAQPLEFLLGGLDKVPVLPVFINGVATPLPGFQRTRMLGEAIGR
                     FTSTLNKRVLFLGSGGLSHQPPVPELAKADAHMRDRLLGSGKDLPASERELRQQRVIS
                     AAEKFVEDQRTLHPLNPIWDNQFMTLLEQGRIQELDAVSNEELSAIAGKSTHEIKTWV
                     AAFAAISAFGNWRSEGRYYRPIPEWIAGFGSLSARTEN"
     gene            complement(6681..8345)
                     /locus_tag="PN977_004267"
     CDS             complement(6681..8345)
                     /locus_tag="PN977_004267"
                     /EC_number="1.14.13.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3-(3-hydroxy-phenyl)propionate/3-hydroxycinnamic acid
                     hydroxylase"
                     /protein_id="HDI6044232.1"
                     /translation="MAIQHPDIQPAVNHSVQVAIAGAGPVGLMMANYLGQMGIDVLVV
                     EKLDKLIDYPRAIGIDDEALRTMQSVGLVDNVLPHTTPWHAMRFLTPKGRCFADIQPM
                     TDEFGWPRRNAFIQPQVDAVMLEGLSRFPNVRCLFSRELEAFSQQDDEVTLHLKTEEG
                     QRETVKAQWLVACDGGASFVRRTLNVPFEGKTAPNQWIVVDIANDPLSTPHIYLCCDP
                     VRPYVSAALPHAVRRFEFMVMPGETEEQLREPQNMRKLLSKVLPNPDNVELIRQRVYT
                     HNARLAQRFRIDRVLLAGDAAHIMPVWQGQGYNSGMRDAFNLAWKLALVIQGKARDAL
                     LDTYQQERRDHAKAMIDLSVTAGNVLAPPKRWQGTLRDGVSWLLNYLPPVKRYFLEMR
                     FKPMPQYYGGALVREGEAKHSPVGKMFIQPKVTLENGDVTLLDNAIGANFAVIGWGCN
                     PLWGMSDEQIQQWRALGTRFIQVVPEVQIHTAQDNHDGVLRVGDTQGRLRSWFAQHNA
                     SLVVMRPDRFVAATAIPQTLGKTLNKLASVMTLTRPDADVSVEKVA"
     gene            8536..9369
                     /gene="mhpR"
                     /locus_tag="PN977_004268"
     CDS             8536..9369
                     /gene="mhpR"
                     /locus_tag="PN977_004268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator MhpR"
                     /protein_id="HDI6044233.1"
                     /translation="MQNNEQTEYKTVRGLTRGLMLLNMLNKLDGGASVGLLAELSGLH
                     RTTVRRLLETLQEEGYVRRSPSDDSFRLTIKVRQLSEGFRDEQWISALAAPLLGDLLR
                     EVVWPTDVSTLDVDAMVVRETTHRFSRLSFHRAMVGRRLPLLKTASGLTWLAFCPEQE
                     RKELIEMLASRPGDDYQLAREPLKLEAILARARKEGYGQNYRGWDQEEKIASIAVPLR
                     SEQRVIGCLNLVYMASAMTIEQAAEKHLPALQRVAKQIEEGVESQAILVAGRRSGVHL
                     R"
     gene            9446..10528
                     /gene="lacI"
                     /locus_tag="PN977_004269"
     CDS             9446..10528
                     /gene="lacI"
                     /locus_tag="PN977_004269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414879.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor LacI"
                     /protein_id="HDI6044234.1"
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAE
                     LNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERS
                     GVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACANVPALFLDVSDQTPINSII
                     FSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREG
                     DWSAMSGFQQTMQMLNEDIVPTAMLVANDQMALGAMRAITESGLRVGADVSVVGYDDT
                     EDSSCYIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLPPNT
                     QTASPRALADSLMQLARQVSRLESGQ"
     gene            10651..>10990
                     /gene="lacZ"
                     /locus_tag="PN977_004270"
     CDS             10651..>10990
                     /gene="lacZ"
                     /locus_tag="PN977_004270"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414878.1"
                     /note="forms a homotetramer; hydrolyzes lactose
                     disaccharide to galactose and glucose; converts lactose to
                     allolactose which is the natural inducer of the lac
                     operon; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HDI6044235.1"
                     /translation="MTMITDSLAVVLQRRDWENPGVTQLNRLAAHPHFASWRNSEEAR
                     TDRPSQQLRSLNGEWRFAWFPAPEAVPESWLDCDLPDADTVVVPSNWQMHGYDAPIYT
                     NVTYPITVNPP"
BASE COUNT         2536 a         3255 c         2837 g         2362 t
ORIGIN      
        1 actcgcaaag cgtcgcatca ggcatctgca cacgatttat ctatgtttac cacatcaaat
       61 catcaggcac tttgaagtca gcatacggat cgtcttcatc ctgctcttcg gcgctgagcg
      121 cgctgtgcaa cgcaatactg ctggcatcac gctgagcaat tttatcggca acgctggcgg
      181 gaataatggc gtattcaccc tcactattgt tatcgaccaa caagcgggca atcgccagac
      241 gaccattaat caactgcgtt tgcgtgagct tatcgacaaa aatcttctta atcagattgc
      301 cgtcagtgaa gttaaaacca atatcgccat tggcaatggt aattcggttc atttcaatga
      361 gctgcttcac ctgggcttta tattctttcg ccaacgccgc ttgtttttgt tgttcgctaa
      421 gctgtttatc acgctcaagc tgtgcctttt tattttcttc taccgccgcc cgagcttcac
      481 gcgcctgaac gcgcgatttt ttcgccgttc tctcgacctt cgccgctttt ttgctggtga
      541 ctaatcctgc tttgagcaac tgctcttgta aggtaagttt tgccatcttt gtttccaaac
      601 ccgctgaata atttttagcg agtatacctg taaacgccgt aggtgtattg gggggaagtc
      661 cctttcgccc ctgcttaata aggcgcgtca ggcatctgca cacggctgca ttcgtgatct
      721 ccggctcatc aaaataaaca ctgccaggcc ggcaacaaga atacccggcg cagaagcggc
      781 cattacgcca accgtgccag tgcctaatgc cagcattttc ccggccagta acggaccgct
      841 catagccccc agacgcccta ccgccacggc tgttcccaca cctgttgcgc ggatctgcga
      901 actgtaaaac aacggtgcca gggcatacaa aacgctttgc ccacctgtcg caaacaaccc
      961 cgcgacgaat cccgccagca acataccgtt aaatgacgac accgttccca gcgccagcag
     1021 cgaagctaac atgccgctat aaatcagtag cgacatggtt actggacgca gcttatccat
     1081 caatgcgccc aacattaacg tcccgcttgc cgcccccatt tgcagtgcaa acatcacccc
     1141 tgccgcctgc gatggctgga atccttgctc caccaaaagc agcggtagcc agttgatcaa
     1201 catgtagacc accagcagag tgaagaaata acacaaccac agcagcagcg tcgcggtagc
     1261 cgtttctggc gcaaataacg cacgcagtgg tggcgcggcc tgtttttcgc cagcgaacac
     1321 cgccgactcc ggcagccagc gcattaacag cggcaccaga atcaacggca ccacaccacc
     1381 tacccaaaac accgtttgcc atgctaagtt tgcccccgcg aaacccagtg tcgccgccag
     1441 cgccgcgcca atgggaacac cgcaatacat caggctcact gccgtcccac gaaaacgtgg
     1501 acccgcggct tcagacgtca gggcgatcag attcggcaac gccgccccca gcccgacacc
     1561 ggtcatcagc cgcgcaaaga ccagtgaggg gaaatcccag gcaatcgccg ttgccagtga
     1621 gaacaaacca aacagcgcaa ctgagccaat caaaatgcgc ttgcgaccat aacggtccgc
     1681 cagcatcccg ccaaccagcg cgccgggtag caaaccgagt attccggcgc tgaatatcca
     1741 gcccatttgc attttatcga gtgcaaaagc ctgggcgatg ccacccgccg caatgccagc
     1801 cgcctgaaga tccagccctt ccatcagagc gaccaaaaaa caaagcccga tggtcagcat
     1861 caggcgggat gaagatgatg aaggggtacg agtcgacata agatgaacct cgttgcgagt
     1921 gcaggtgagt cgcggctgat acattgccag ccgcgtaatc agaaccattt aatgactgcc
     1981 tacgggcgaa atataggccg gacaagacgt ttacgccgca tccggcagtc gtgctgggat
     2041 gggcatacaa aatgcacgtt ctcggcagtc atcattattt gttgttgcgc agatccagcg
     2101 ccacgtcgac gatcatatcc tcctggccgc caaccatccg gcgtttgccc aactcaacga
     2161 gaatatccac cgcgcttaag ccataacgcg ccgccgccgt ttcacagtga cgcaggaagc
     2221 tggagtaaac accggcgtat cccagcgcca gcgtttcgcg atcgactcgt accggacgat
     2281 cctgcaacgg acgcaccagg tcgtcagcgg catccattaa cgcatagaga tcggtcccat
     2341 gctgccaacc cagtttatcc gcggcggcaa taaacacttc cagcggcgcg ttacctgcgc
     2401 ccgcgcccat tcccgcgaga ctggcatcga ttcggtcgca gccctcttcc accgccgcga
     2461 tagagttcgc cacgccaaga ctcaggttat ggtgagcgtg tatgccggtt tgcgtttctg
     2521 gtttcagcac tgctttcagg gcgcggaaac ggtcacggat atcgctcatg ttcatcgcac
     2581 cgccagaatc caccacataa atacaggtcg caccgtagcc ttccatcagc tttgcctgct
     2641 ttgcgagatt ctccggcgtg gtcatatggc tcatcatcag aaagccaacg gtgtccattc
     2701 cgagctcgcg ggcatactga atatgctggg cggaaacatc agcttcggta cagtgcgtag
     2761 ccacacgaac cacccgcgcg ccagcctgcc aggcattttt cagatcgtga atagtgccga
     2821 ttccaggcag caacaacgtc gcgattttgg cgtgcttcac cacatccgcc gccgcttcaa
     2881 tccattcaag gtcgctatgt gcgccgaaac catagttaaa gctggaacct tgcaaaccgt
     2941 cgccgtgggc cacttcaatc gaatccacgc gggcatcgtc cagtgctttg gcaacctggc
     3001 gaacgttttc caatgaatac tgatgacgaa tggcgtgcat accatcacgc aacgtgacgt
     3061 ccgagatata aagtttttta tcgttcatgc cgcttctcct gccttgcgcg ccagtgactg
     3121 ggccattttt tccgctgtcg ccagcgcact ggaagtcata atgtcgaggt tgcccgcata
     3181 ggcaggcaga taatgtgctg cgccttcgac ttccagccag acagccgttt tcagcccgga
     3241 gaactgcccc acgcccggca aattaaccgg tttatcctgc gggataactt caaactgcac
     3301 gcgctgtttc aggcgatatc ccggtacgta agcctgcacc gcctcagcca tttcattgat
     3361 tgaggcttcg atatcatctt gtgaagcttc gtcgctcaat acatacaccg tgtcacgcat
     3421 catcaacggt ggctctgctg ggttaagcac gataattgcc ttcccttttg ccgcgccgcc
     3481 caccacttca atggctcggg aagtggtttc cgtaaattca tcgatattgg cacgcgtgcc
     3541 aggtccggca gatttgctgg cgatagaagc gataatttcg gcgtaatgaa cacgcgccac
     3601 gcgtgaaact gccgccacca ttggaatggt ggcctggccg ccgcaggtga ccatgttgac
     3661 gttcaattga tcgacattcg cctcgagatt aacgaccggc acgcagtaag ggccgatggc
     3721 agcaggcgtc aggtcaatta agcgaatatc cggtttcgct tcgcgcaaag cggcatcgtt
     3781 tttcacatgc gcgcccgcgc tggtcgcatc aaatacaatg tcgatatcag caaattcagg
     3841 catgttcatc agcccgatca ccccttcatg ggtggtggcg acgcccatac gtctggcacg
     3901 cgccagaccg tcggactgag gatcaatgcc gaccatcacc gccatctcca gatgctgacc
     3961 gtgacgcaaa attttaatca tcagatcggt gccaatgttg cctgaaccga taatggcgac
     4021 tttacgctta ctcatgacag acttcctttt ggggctgcgc ttgaaaatgt cgcagcaact
     4081 gaacctatgc cttcgatatg ggcttcaaaa cgatcgcccg cattcaccgc caccatcgga
     4141 cctaatgccc cggtaaggat gatatctccg gcgcgcagcg gttcacccag actggccatt
     4201 ttgcgtgcca gccagacggc ggcattaagc ggatgtccca ggcattcgct gccgcgcccg
     4261 ctggaaacct cttcgttatt acgcgtcatc ttcatggcgc agtttttcag gtctaacccc
     4321 gccgggcgtt gcgccggacc gccgatgaca tacaccccgc aggaagcgtt atctgccacg
     4381 gtatcgacaa actgaatcga ccagtcgcga atgcggctcc ccaccacctc cagcgccgga
     4441 agtacccatt caatggcgtt atacaattcg tcgaaggtga tatcggttgc gggtagatcg
     4501 cggttcaaca ccagtgcgat ctccgcttca atgcggggtt gcagaacacg ggaaaaagga
     4561 atggtttcat tatcgccata acacatgtcg gcaaataatg tcccgaaatc cggttgatca
     4621 acgcccagtt gttgctgcac tttcggatgc gtcagcccca ctttacgccc caccacgcga
     4681 cgcccctgcg cgacgtcatg ttgcacattg atgtgctgaa tggcgtaggc cgcttcagcg
     4741 ttatcgatac caatcagatc gcgcagcggc gcaatcgctt cgccctgctc tgcggcgcgg
     4801 cgtaaatccg ccgccagttg ctcaagagta tgcttcgtca tgaccgttcc ttaagggcgt
     4861 gcgaggaaat tcagcaccag ttgattgaaa gcgtcggcat gttcccactg cgcccagtga
     4921 ccacagtcgc ggaagatatg cagttcagaa ccggcaatgc cggacagcag acgcagaccc
     4981 gcatccatcg gcacaaagcg gtcgttgcgc ccccagacaa tcagggtttg cgctttgatt
     5041 tccgccagcc gtgggccaaa atccgggaac tgtttcggat tggcttccag gctcttaacg
     5101 aagttttcca ggtgatcgcg gcgcgacagc atattattca gacgcgcttc aaacagggcg
     5161 tcggtcaaat cgctggtatc aaaaacgaag atatccatca tcagcttcag gttttcgata
     5221 gtcggctgac gataaagctg attcagtcgc ttaatacctt cggttggcat cggcgtaaac
     5281 aaactcatgc cgcccgtacc gccgcccatc agcaccagtt tgccgacgcg ctccggccag
     5341 ttaagggtga acgccacaga actatggcca cccatcgagt tgcccagcag gtggattttg
     5401 gcgatatcca gttgatccac cacgcttttc aggattcgtg cattaagatc cgatcgcgaa
     5461 ccactattaa cgatcgaatc gctcttgccc caacccggac aatccagcag gatcacccga
     5521 tagcccgcct ctaccagcgg atcgatattg cggctgaagt tcgcccagcc agtagcaccc
     5581 gggccggaac catgcagcag gacaacagtt tcgtcgcctt gtccgcagtc attaaaatgg
     5641 atgcgcagcg ttttacccgc ttcttctaca ttcagaaaac ggctggtggc ggcttcggtt
     5701 tgtggctgat aactcatcat cttctcctgc atattcagtt ctctgttctg gcgcttaacg
     5761 agccaaatcc ggcaatccac tccgggattg ggcggtaata acgcccttcg ctacgccagt
     5821 tgccaaacgc agaaatagcg gcaaaagcgg cgacccaggt tttgatttca tgtgtcgact
     5881 ttccggcaat ggcggaaagc tcttcgttac tgacggcatc cagttcctgt atgcgtccct
     5941 gctccagcaa agtcatgaac tggttatccc aaatcgggtt gagcggatgc agcgttctct
     6001 gatcctcaac aaacttctca gcggcgctaa tcacccgttg ctgacgcaat tcgcgctcac
     6061 tggcgggtaa atctttcccg ctccccaaca gacggtcgcg catatgggca tcggctttcg
     6121 ccagttcggg caccggcggc tgatgggaaa gcccaccgga ccccaggaac agcacgcgtt
     6181 tattgagagt actggtgaaa cgtccaatgg cttcccccaa catgcgggta cgctggaaac
     6241 cgggcagcgg cgtggcgaca ccgttgatga acaccggcag tactggcacc ttatccagcc
     6301 caccgagcag gaactccagc ggctgggcga acccgtggtc cacctgcata cagtaagaaa
     6361 cggcaagatc gatcccgctc ttcatgacgg catgcgcaca ggcctccgcc agctccacag
     6421 gcacgggcag ctctcctgcc gcactgccga aatcaccaat tgccgtcgct ccaacgccta
     6481 aacagaacgg tggcatcacg tcatagaaaa agccgttgta gtgatctggc gcaaacagca
     6541 ccaccagttc aggggagaaa gccgcaatac gctcgcgggc gctggcaatc acgccattga
     6601 cctcatcgag tacctcttgc gccgggtcga catatcccac cagcggcgag tgggaaagac
     6661 agtgaagata agcgtgcata tcaggctacc ttttcgacag aaacgtcggc atcagggcgg
     6721 gtcagcgtca tcaccgacgc cagtttattc agggtcttgc ccagggtttg cggaatggcg
     6781 gtggcggcaa caaagcgatc cgggcgcatc accaccagcg aagcattgtg ttgcgcgaac
     6841 cagctacgca ggcgaccttg cgtatcgccc acgcgtagta cgccgtcgtg gttatcctgt
     6901 gcggtatgaa tttgcacttc cggcaccacc tgaatgaagc gtgtgcccaa cgcgcgccac
     6961 tgctggattt gctcgtcgct catcccccac agtggattgc atccccagcc aattaccgcg
     7021 aagttcgcgc cgatcgcgtt atcgagcagc gtcacgtcgc cgttttccag cgtgactttc
     7081 ggctgaataa acatcttgcc gaccggagag tgcttcgctt cgccctcacg caccagcgca
     7141 ccgccgtaat attgcggcat cggcttgaag cgcatttcga ggaagtagcg ttttactggc
     7201 ggcagataat tcaacagcca ggaaacgccg tcacgtaacg taccctgcca gcgtttcggc
     7261 ggagccagca cgttgcccgc cgtcacggac aggtcaatca tcgctttggc gtgatcgcga
     7321 cgttcttgtt gataggtatc gagcagcgca tcgcgggctt tcccctggat aaccaacgcc
     7381 agtttccatg cgaggttaaa ggcgtcgcgc ataccactgt tatagccctg cccctgccac
     7441 accggcatga tgtgcgcggc atcgcccgcc agcagtacgc gatcaatacg aaaacgttgc
     7501 gccagtcgcg cgttgtgggt gtagacacgc tggcgaatca attcaacatt gtccggatta
     7561 ggcagcactt tgcttaacag cttgcgcata ttttgcggct cacgcagctg ttcttcggtt
     7621 tctcccggca tcaccataaa ttcaaagcga cgtaccgcat gaggcagcgc ggcagaaaca
     7681 tacgggcgca ccggatcaca acacaaatag atatgcggcg tacttaacgg atcgttggcg
     7741 atatctacca caatccactg atttggcgca gttttacctt caaacggcac attcagggtg
     7801 cgacggacaa aacttgcccc accatcacag gccaccagcc actgggcttt gaccgtttcc
     7861 cgctgccctt cttccgtttt taggtgcaag gtcacttcgt catcttgctg gctgaaggcc
     7921 tccagctcgc gggaaaacag gcagcgcaca ttcggaaaac gcgacaatcc ttccagcatc
     7981 accgcatcga cctgaggctg aataaaggcg ttacggcgcg gccagccaaa ttcatcggtc
     8041 attggctgaa tatcagcaaa acagcgacct tttggggtga gaaaacgcat cgcgtgccac
     8101 ggcgtagtgt gcggcagaac attatcgacc aggccaaccg actgcatggt gcgcagcgcc
     8161 tcgtcatcaa taccaatcgc acgcgggtag tcgatcaact tatcgagttt ctccaccacc
     8221 agcacgtcaa tgcccatctg gccgagatag ttcgccatca tcagcccgac cggaccggca
     8281 ccagcgatcg ccacctgaac gctatggtta acagcaggct ggatgtcagg gtgttgtatt
     8341 gccatttcag tacctcacga ctcggacaaa atgccgttgc gcgcacagta cagcgcaact
     8401 tgttttgtta aaaacatgta aatgattttt tattgtgcgc tcagtatagg aagggtgttt
     8461 tcggctacaa tcaaaacatg cccgaatgtg caccaggtgc acaacgttgt tttaactata
     8521 taaatgtcaa ttaatatgca gaacaatgag cagacggaat acaaaaccgt gcgcggctta
     8581 acccgcggtc taatgttatt aaatatgtta aataaacttg acggcggtgc cagcgtcggg
     8641 ctgctggcgg aactcagcgg cctgcatcgc accactgtgc ggcgactgct ggagacgctg
     8701 caggaagagg gatatgtccg gcgtagtccc tccgatgata gtttccgttt gaccatcaaa
     8761 gtgcggcaat taagcgaagg atttcgtgac gaacagtgga tttctgcact ggctgcccca
     8821 ctgctgggcg atctgttgcg cgaagtggta tggccgacag atgtgtccac gctggatgtt
     8881 gatgcaatgg tggtacgcga aaccacccat cgtttcagcc gattatcctt tcaccgggca
     8941 atggtcgggc gacggttacc gcttctgaaa accgcctcgg gcctgacctg gctggccttt
     9001 tgcccggaac aagaacgcaa ggaattaatc gagatgttag cctcccgccc cggtgatgac
     9061 tatcaactgg cacgagaacc gttaaagctg gaagccattc tggcgcgcgc gcgcaaagag
     9121 gggtacggac agaactaccg cggctgggat caggaggaga agatcgcctc tatcgccgta
     9181 ccgctgcgca gtgaacaacg ggtgattggc tgtctgaatc tggtgtatat ggcgagcgca
     9241 atgaccatcg aacaggcagc ggaaaagcat cttccggcgc tgcaacgggt ggcaaaacag
     9301 atcgaagaag gggttgaatc gcaggctatt ctggtagcgg gaaggcgaag cggcgtgcat
     9361 ttacgctgac accatcgaat ggcgcaaaac ctttcgtggt atggcatgat agcgcccgga
     9421 agagagtcaa ttcagggtgg tgaatgtgaa accagtaacg ctatacgatg tcgcagagta
     9481 tgccggtgtc tcttatcaga ccgtttcccg cgtggtgaac caggccagcc acgtttctgc
     9541 gaaaacgcgg gaaaaagtgg aagcggcgat ggcggagctg aattacattc ccaaccgcgt
     9601 ggcacaacaa ctggcaggca aacagtcgtt gctgattggc gttgccacct ccagtctggc
     9661 cctgcacgcg ccgtcgcaaa ttgtcgccgc gattaaatct cgcgccgatc aactgggtgc
     9721 cagcgtggtg gtgtcgatgg tagaacgaag cggcgtcgaa gcctgtaaag cggcggtaca
     9781 caatctcctc gcgcaacgcg tcagtgggct gatcattaac tatccgctgg atgaccagga
     9841 tgccattgct gtggaagctg cctgcgctaa tgttccggct ttatttcttg atgtctctga
     9901 ccagacaccc atcaacagta ttattttctc ccatgaagac ggtacgcgac tgggcgtgga
     9961 gcatctggtc gcattgggtc accagcaaat cgcgctgtta gcgggtccat taagttctgt
    10021 ctcggcacgt ctgcgtctgg cgggctggca taaatatctc actcgcaatc aaattcagcc
    10081 gatagcggaa cgggaaggcg actggagtgc catgtccggt tttcaacaaa ccatgcaaat
    10141 gctgaatgag gacatcgttc ctactgcgat gctggttgcc aacgatcaga tggcgctggg
    10201 cgcaatgcgc gccattaccg agtccgggct gcgcgttggt gcggatgtct cggtagtggg
    10261 atacgacgat accgaagaca gctcgtgtta tatcccgccg ttaaccacca tcaaacagga
    10321 ttttcgcctg ctggggcaaa ccagcgtgga ccgcttgctg caactctctc agggccaggc
    10381 ggtgaagggc aatcagctgt tgcccgtctc actggtgaaa agaaaaacca cccttccgcc
    10441 caatacgcaa accgcctctc cccgcgcgtt ggcagattct ttaatgcagc tggcacgaca
    10501 agtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt cagctcactc
    10561 attaggcacc ccaggcttta cactctatgc gtccggctcg tatgttgtgt gaaattgtga
    10621 gcggataaca atttcacaca ggatacagct atgactatga ttacggattc actggccgtc
    10681 gtattacaac gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg ccttgcggca
    10741 catccccatt tcgccagctg gcgaaatagc gaagaggccc gcaccgatcg cccttcccaa
    10801 cagttgcgca gcctgaatgg cgaatggcgc tttgcctggt ttccggcacc agaagcggtg
    10861 ccggaaagct ggctggattg cgatcttcct gacgccgata ctgtcgtcgt cccctcaaac
    10921 tggcagatgc acggttacga tgcgcccatc tacaccaacg tgacctatcc cattacggtc
    10981 aatccgcctt
//
LOCUS       DAMZFT010000154        10947 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.154, whole genome shotgun sequence.
ACCESSION   DAMZFT010000154 DAMZFT010000000
VERSION     DAMZFT010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10947)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10947)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10947
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.154"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            644..1621
                     /locus_tag="PN977_004271"
     CDS             644..1621
                     /locus_tag="PN977_004271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HDI6044236.1"
                     /translation="MDKSSGELVTLTPNNNNTVQPVALMRLGVFVPTLKSLKNSKKNT
                     LSRTDATEELTRLSLARAEGFDKVEITGPRLDMDNDFKTWVGIIHSFARHNVIGDKVE
                     LPFVEFAKLCGIPSSQSSRRLRERISPSLKRIAGTVISFSRTDEKHTREYITHLVQSA
                     YYDTERDIVQLQADPRLFELYQFDRKVLLQLKAINALKRRESAQALYTFIESLPRDPA
                     PISLARLRARLNLKSPVFSQNQTVRRAMEQLREIGYLDYTEIQRGRTKFFCIHYRRPR
                     LKAPNDESKENPLQPSPAEKVSPEMAEKLALLEKLGITLDDLEKLFKSR"
     gene            complement(1906..2646)
                     /locus_tag="PN977_004272"
     CDS             complement(1906..2646)
                     /locus_tag="PN977_004272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDI6044237.1"
                     /translation="MNNVIPLQNSPERVSLLPIAPGVDFATALSLRRMATSTGATPAY
                     LLAPEVSALLFYMPDQRHHMLFATLWNTGMRIGEARMLTPESFDLNGVRPFVRILSEK
                     VRARRGRPPKDEVRLVPLTDISYVRQMESWMITTRPRRREPLWAVTDETMRNWLKQAV
                     RRAEADGVHFSIPVTPHTFRHSYIMHMLYHRQPRKVIQALAGHRDPRSMEVYTRVFAL
                     DMAATLAVPFTGDGRDAAEILRTLPPLR"
     gene            complement(2767..2949)
                     /locus_tag="PN977_004273"
     CDS             complement(2767..2949)
                     /locus_tag="PN977_004273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001312821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein kinase"
                     /protein_id="HDI6044238.1"
                     /translation="MHVAQLPSLCIFLLSLRSSLPWHYPISPKNGKPALSPRHDWLNT
                     GSLLQNIPLRIWFYPQ"
     gene            complement(3292..3599)
                     /locus_tag="PN977_004274"
                     /pseudo
     CDS             complement(3292..3599)
                     /locus_tag="PN977_004274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461876.3"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system ParD family
                     antitoxin"
     gene            4092..5261
                     /locus_tag="PN977_004275"
     CDS             4092..5261
                     /locus_tag="PN977_004275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001072358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6044239.1"
                     /translation="MNQKWKTLIISLLTPSSIISGIALIVLWGYFSRLGRLDIFFDVM
                     NIKSILVLVCCATILSLAMIVFIFFITSFFIPVVIPQDINNLPAYNKIQGNFLSVMML
                     SGMFPMAFIYVLYCAFDFDQNVKDNSGWLSIASMGVLIAVILAIVNKRYLEYDLSFKN
                     NRMKLLRRVQIYLVIPLSIALLVHLQLIPLEIVFSNIATSDKSVNFKVIAELAFMSYF
                     IFLLTMLPGVIYLKMNPQHKFSKRISYSFIASLMLLLIISTQITVLPVIFTHSVIKLS
                     GISDFKIHSYIIKTSEYPEEFFSNAAWDKKNIKPGDYYSVQAVSMFTTNQFILLCPKD
                     IIKFYRESWKFELLNVDFDINTRKKLQEEAAYCVPISAISVKRWDMPLQGSKPSS"
     gene            5294..5422
                     /locus_tag="PN977_004276"
                     /pseudo
     CDS             5294..5422
                     /locus_tag="PN977_004276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014543172.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS481 family transposase"
     gene            complement(5457..5750)
                     /locus_tag="PN977_004277"
     CDS             complement(5457..5750)
                     /locus_tag="PN977_004277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6044240.1"
                     /translation="MNYDEITKITAERISDYMTEAVNTDSIAVAEMFHNAAWGARTLW
                     FELVTKIDIDIHKKNRYASYDLRRKIEMQHEEFQKMTEREQVPLLKCISSDLI"
     gene            complement(5856..6131)
                     /locus_tag="PN977_004278"
     CDS             complement(5856..6131)
                     /locus_tag="PN977_004278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HDI6044241.1"
                     /translation="MELKWTSKALSDLSRLFDFLVLASKPAAARTVQSLTQAPVILLT
                     HPRMGEQLFQFEPREVRRIFAGEYEIRYEINGQTIYVLRLWHTRENR"
     gene            complement(6131..6415)
                     /locus_tag="PN977_004279"
     CDS             complement(6131..6415)
                     /locus_tag="PN977_004279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix protein, CopG family"
                     /protein_id="HDI6044242.1"
                     /translation="MQMKNNAAQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQA
                     LSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQAWSDSLSTDNPLPVPR"
     gene            7020..7772
                     /locus_tag="PN977_004280"
     CDS             7020..7772
                     /locus_tag="PN977_004280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000562172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HDI6044243.1"
                     /translation="MIATKTPAIDKAARIFHFIAGKGSATYSEIHQNVGLPQSTTSAL
                     LASLTTHAFLRQKNGRYYLGLIFYELGNKAIQDFDFRELAEESLVFLRDQTRLACHLG
                     VLDGSTGIYLAKKESQNAIIVRSWTGKRLSLHSSSLGKVLLAWLPEDHIDALLPTEHL
                     PAFTARTITTRKALKAELEVVRLQGWAFDNQEDVDDVTCIAAPVFNSDQAVVAAISIS
                     GVRFQMPEEKIPEYANAVMKAAGMMSEKLCGI"
     gene            complement(7818..8783)
                     /gene="kdgT"
                     /locus_tag="PN977_004281"
     CDS             complement(7818..8783)
                     /gene="kdgT"
                     /locus_tag="PN977_004281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001022265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxygluconate transporter"
                     /protein_id="HDI6044244.1"
                     /translation="MNIKKTVEKIPGGMMLAPLFLGAVLHTFWPGTGKYFGSFTNGMI
                     TGVVPILAVWLFCMGASIKISATGTVLKKSGTLVATKIATAWVCAFVFAQLLPEGGMV
                     KTGFFAGLSVLAIVAAMDMTNAGLYASLMQEYGTKEEAGASVLISLESGPLMTMIILG
                     SAGQATFEPEHLAGVLIPFLVGFLLGNLDPELRELFSRATKSLIPFFAFALGNTINLG
                     VIIDTGLLGILMALAVIVITGVPLIIMDIMLGKGRGTAGIAASSTAGAAVATPLLVAE
                     IAPDFAEAAPAATTLVASCVVITAIVVPVITALWAKHGASRVRAT"
     gene            complement(8816..9196)
                     /locus_tag="PN977_004282"
     CDS             complement(8816..9196)
                     /locus_tag="PN977_004282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HDI6044245.1"
                     /translation="MKKDIIYTTGAPAPGGALSQAVKAGETIYLAGQVGFDPHTMQVV
                     STDFDEQARQAFKNLLSVAEAAGGSDRDIVKLNAYLTDVNMFPRFNAIMSEYFSPPYP
                     ARATLGIAALPQGAVIEIEAIMVL"
     gene            complement(9221..10111)
                     /locus_tag="PN977_004283"
     CDS             complement(9221..10111)
                     /locus_tag="PN977_004283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001077068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HDI6044246.1"
                     /translation="MNQTMKFSGAVCPSVIPLDDSGQIDLTGLEKHMARLSDSGINGI
                     LLSGTIGEFATMSVRERAMLILEARKMSDLPLIAHISSTVEEDMLYLADAAYDSGYDA
                     VMVLPQYYYAQTSRQLLSYFRSLDRKLAGDWFIYNFPARTGCDVDAHLVRMLAESCPR
                     FIGIKDTVDCASHTRAIVNAVTPLRKDFAVFAGFDEYFVPNLMNGGAGVLSGLNNVVP
                     ELFAQIMRAYRAGDLNEVAGLHKEIGRLSGIYAIGDDFVSTIKTAISRKYGYMTPVSR
                     NHNGQLTADQADCLDKLFGL"
     gene            10512..10947
                     /locus_tag="PN977_004284"
                     /pseudo
     CDS             10512..10947
                     /locus_tag="PN977_004284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224837.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
BASE COUNT         2808 a         2616 c         2637 g         2886 t
ORIGIN      
        1 ctgtagtttg tatacacaat ccctggcatt gaaacacaca tatgaagtgt acttcggata
       61 tgaccgtacg ctcaggaagt acggcagatt ccgtcaaaaa ttacctgtaa agacagacat
      121 cagaaaaaac agtgctcgcc gcagaccggt gaccgggcga agcccgcgaa ccggcgagga
      181 agcgcaatgt atctgtagtt acagggagca ggtacgccct gaagcccttt cataaaacgg
      241 ttgtgtgcgc tgtccttctg gaatgattta tccacatatc cggaggcccg atccagtaaa
      301 cagatccatg aatgatcaac aaaggatcca ttaaagatcc ccatatcgct gcaaaccttg
      361 tcactcatgg gccgggacca cgatcacata agctgtggca tgttactgat aaactgtaac
      421 atgctaatga taagctgtat tcagtaatcc atatactgaa gtaagttaat gacataaact
      481 atggtcagta tgccagactc agttgttaaa tacaggctgc aggtttttct tcagtcagtt
      541 agcggagttc tgacacacga ttttctgttt attcttttac tgtccacagg ctggaggctt
      601 tctggaaaac gaaaattcag acatcaaaaa actgttcggc gaggtggata agtcctccgg
      661 tgagctggtg acactgacac caaacaataa caacaccgta caacctgtgg cgctgatgcg
      721 tctgggcgtt tttgtaccga cccttaaatc actgaagaac agtaaaaaaa atacactgtc
      781 acgtactgat gccacggaag agctgacacg tctttccctg gcccgtgctg agggattcga
      841 taaggttgag atcaccggcc cccgcctgga tatggataat gatttcaaga cctgggtggg
      901 gatcattcat tcctttgccc gccataacgt gattggtgac aaagttgaac tgccttttgt
      961 tgagtttgca aaactgtgtg gtataccttc aagccagtca tcccgcaggc tgcgtgagcg
     1021 catcagccct tccctgaagc gcattgccgg taccgtgatt tcgttttccc gtaccgatga
     1081 gaagcacacc cgggaatata tcacccatct ggtacagtca gcttactacg atactgagcg
     1141 ggatattgtg cagttacagg ccgatccccg cctgtttgaa ctgtaccagt ttgacagaaa
     1201 agtccttctc cagcttaagg cgattaatgc cctgaagcga cgggagtccg cccaggcact
     1261 ctacaccttt atagagagcc tgccccggga tccggcaccg atatcgctgg cgcggctgcg
     1321 tgcacgcctc aatctgaagt ctcctgtatt ttcccagaac cagacggtca gacgggcaat
     1381 ggagcagttg cgcgagattg gatatcttga ttacacggag atccagcggg ggcggacaaa
     1441 attcttctgt attcactacc ggcgtccccg gttaaaagcg ccgaatgacg agagtaagga
     1501 aaatccgttg caaccttcac ctgcggaaaa agtcagtccg gagatggcgg agaagcttgc
     1561 cctgcttgag aaactgggca tcacgctgga tgacctggaa aaactcttca aatcccgctg
     1621 aacataaact gtagtcagtg aagagcgctc ctttactgac tacagcttat attaaccggt
     1681 gcagtgagtg gtctgctcac tgcagtttat attctgtttc ctgcagtgct gtctgtagct
     1741 gagctgccat ctgcctgtcc cttacgtaag tgatcccgta acctgatgct gaggcattgc
     1801 tcccttcata aaacatgact tactcactac agcttatata catgctccag cttatgttat
     1861 gtctgttctg ctgaccacag cttatataag gaagcgcgta tgccgtcacc tcaggggagg
     1921 cagtgtacgc aggatctccg cagcatcccg cccgtcacct gtgaaaggca ctgccagcgt
     1981 ggcagccata tccagtgcaa acactctggt ataaacctcc atcgaacgtg gatccctgtg
     2041 accagccagt gcctggatga ctttccgggg ctggcggtga tagagcatgt gcatgatata
     2101 gctgtgccgg aaggtgtgtg gtgtgaccgg aatcgaaaag tgtaccccgt cagcttcggc
     2161 ccgtctgaca gcctgcttca gccagttgcg catggtttcg tcggtcacgg cccataatgg
     2221 ttcacgacga cggggccggg tggtgatcat ccagctttcc atctgcctga catagcttat
     2281 atctgtcagc ggcaccagtc gcacttcatc ttttggcggg cgtccgcgtc gcgcacgcac
     2341 tttttcggac aggatccgca caaacggtct tactccattc aggtcaaatg attccggcgt
     2401 cagcatccgg gcttcgccaa tacgcattcc ggtattccag agggtggcga acagcatatg
     2461 gtgacgctga tccggcatat agaagagaag ggcactcact tccggggcca gcaggtaggc
     2521 cggtgtggcc cccgtggaag tggccattct tctcagggag agcgctgttg caaaatccac
     2581 ccccggcgca atgggtaaca gggagacgcg ttctggtgaa ttctgcaggg gaatgacatt
     2641 gttcatgagt tatttttcac gtagcacacg cactgtttat ggatacaggt attctgacct
     2701 gaacgcccgg atccttccac gacaataaat cgcagcgcgc ggatcctttt gtttgcttat
     2761 cacgcattat tgcgggtaaa accaaatacg taaaggtatg ttttgcagca gggatcccgt
     2821 attaagccag tcgtggcgcg ggctgagggc tggtttgccg tttttgggag aaatggggta
     2881 atgccagggc aaagatgatc gcaaggaaag gagaaaaata cacagagagg ggagttgcgc
     2941 tacgtgaatt ctggctaggt acacccctgt aaccctgcca gttttcacgc aacgcaatgt
     3001 ggtggctgat tactgtcagt tgaaatgacg gctggcatcc tgatgctgac ttaatatacg
     3061 aataataaca atttcagtat cagtctgcag aaaataaacc atatgccgtt caacgggtag
     3121 tgcgaatgtg tattccccag ttcaggaggg ggagtaccga tattatgatt actcataacc
     3181 tgaaaaatgt cggatatctg attaatatat ttatcagcct actcttcacc gaaatggtgg
     3241 tacccgtaat gctagatatc ctccagatcc tgactggctt taggtgtgag tttaacggtt
     3301 ttcaccggaa gccctcgcgc cagctttcac ttttttcaga aacgcatttt tttcccacga
     3361 cacaggttca ccgctgctca agccttctgc cagtaaatcc cgcaggacac ctggagacgt
     3421 gactctgcct gtttctctcg aaggagacga agtgactcgc ggattacctc actctgtgta
     3481 cgataatcac ctgattctat gagagattct atgtactccc agagttcatc tccgagagca
     3541 actgtcattg ttctggccat atcaagcctc gtgattgtaa gtttaaattt tacatgcagt
     3601 ttattcgata ctcaaaggtg cttccagtac agaacatttg ggagaattgg ggtaatgcct
     3661 ggaacagaca gatacggaga aaaaccctgc agataacctt atacgacaac tgaaggtgtc
     3721 tgatgccata ttcttagcta cttctctgtc ttcaggcggc ttagtcacaa cataagaccg
     3781 atgcataata agcatatttt ttcatgttat ggtcttgttc atgcatgaac tattatctat
     3841 tcagtcactt ttaaagtttt atatcatact gcatatgata tatatcatct cctatattca
     3901 gactaaaaat attatgacga tggtcaaatg aatttagtga aaacgctacc taaaacaata
     3961 tgcttatgat ataacttatc aagaatatgc gacaatcttc ttgaaaggtt acggagaaga
     4021 gtcagataat gaagtgttat aactcattca gcttttttat gttctcttta tagtaaaacc
     4081 aggagattaa gatgaatcag aagtggaaga cgttaattat ttcattatta acaccatcaa
     4141 gtattatatc agggattgcg ttgatagttc tctggggata tttcagtcgc ctgggccgac
     4201 tagatatttt ctttgatgta atgaatatta aaagcatctt ggtattggtt tgttgtgcaa
     4261 ctatactatc attggctatg atagtattta tcttttttat aacctctttt tttatacccg
     4321 tagttattcc tcaggatata aataatcttc ctgcttacaa taagattcag ggtaattttt
     4381 tatctgtgat gatgttatct ggcatgttcc ccatggcctt tatatatgtg ttatactgtg
     4441 catttgattt tgatcagaat gttaaagata actctggctg gttatctatt gcaagtatgg
     4501 gtgtactgat agctgttatt ttagccatag taaataagag gtatcttgaa tatgacttat
     4561 cgtttaaaaa caatagaatg aaactgttga gacgtgttca gatctatctt gtcattccgt
     4621 tgtctattgc tttactggtg catcttcagt taattcctct ggagatagtt tttagtaata
     4681 ttgcaacttc agataagagt gttaatttca aagtgatagc tgaactggct ttcatgtcat
     4741 attttatctt tcttctgaca atgcttcctg gagtcattta tctgaaaatg aatccacagc
     4801 ataaattttc aaaaagaatt agctattcat ttatcgcatc cttgatgtta ttattaataa
     4861 tatcgactca aataaccgtg ttaccagtga tttttaccca ttccgttatt aagctatcag
     4921 gtataagtga cttcaaaata catagctaca ttattaaaac aagtgaatat cctgaagagt
     4981 ttttttccaa tgcagcatgg gataaaaaaa atataaaacc gggggattat tactctgtgc
     5041 aggctgtttc tatgtttacg acaaaccagt ttattctcct ttgtcctaag gatattataa
     5101 agttttatcg ggaaagctgg aagttcgagt tgcttaatgt agacttcgat ataaatacca
     5161 ggaagaaact ccaggaagaa gctgcatatt gcgtaccaat ttctgcaata tctgtaaaac
     5221 gatgggatat gcctcttcag ggctcgaaac cctcaagtta gaaggcaact agggcgactg
     5281 tattctgtga cgaaatgaat ggctggctca ctataataat gagcgaaccc atcagggaaa
     5341 aatgtgcaat gggcggacgc caatggaaac gttactcgat ggaaaacgca tctgggccga
     5401 gaaaaattta agccagatgt aatctgacag atacctgtat aaataaccga taactgtcag
     5461 atcaggtctg agctaataca tttcaataaa ggtacttgct cgcgctctgt cattttctga
     5521 aactcttcat gctgcatttc gattttcctt ctaagatcat aactcgcata cctgttcttt
     5581 ttatgtatat ctatatctat ttttgtgacg agttcaaacc acaatgttcg cgctccccat
     5641 gcagcgttat gaaacatttc cgcaacggct atagagtctg tattaactgc ttcagtcatg
     5701 tagtcgctaa tacgttcagc ggtaattttc gttatttcat cgtaattcat tttatctcct
     5761 acaagattat ttaatggaat gcaaaatatc tggtctgata ataatgttcc agaattaata
     5821 tctgcaggct atgttaagtt gtccccacac acaatctacc tgttttctcg tgtgtgccac
     5881 agacgcaata cataaatagt ctggccatta atttcgtaac ggatttcgta ctcgccagca
     5941 aaaatccgtc tgacctccct gggttcaaac tgaaacaact gttctcccat acgtggatga
     6001 gttaacagaa tgaccggtgc ctgtgtcagg gactgtaccg ttctggcggc agcaggttta
     6061 ctggccagca ccagaaaatc aaataaccgc gacaaatcag aaagcgcctt actggtccac
     6121 ttcagttcca tcagcgtggc accggtaacg gattgtcagt actgaggctg tccgaccagg
     6181 cctgtacagc ctgatggtcg ataacctgtc cggatgtcac atcgtccagg gcttccagcg
     6241 tcaggcgatt acgctcctcc tcctgggcaa gccatgcaga aagcgcctgt ttgataaccc
     6301 agccccggga acgttccagt ctggcggcca tctggtcgac tttatcagcc ataggtaatg
     6361 gcacatgcgc ggtaattact tttgttgctt gtgcggcatt gtttttcatt tgcatatccg
     6421 attagttgtt aatcatattt aatcatagtg attactttca gacggaacaa ggcaaataat
     6481 gcgcgtcagc gcatgtaagg cggcatacgg aggttacatg tagcacaggt gtaaaaccgc
     6541 cgaagcccgg cgtaagccgg tactgattga tagatttcac cttacccttt tccatcctgc
     6601 taagccacac ccgctttcag ctatgagaga gcctttgtgc gcagtcgggg cgtatctccg
     6661 cgttagcggg ccgtgaggga cgcttacgag cgtggatgag agacgtacag tggtttcttc
     6721 tagcttttat ttcctctcag gtcccgaatt gtcaacttct gaatcgtggg ggcccctttg
     6781 gggtgacgat tcagtggttg acaactgcac ataaaacacc ctgtgtgtgg ggtgatggtt
     6841 cacgtagtgg tccatcgccc tgccaggggt cagtcgccac tacaccatca cttgagaaag
     6901 agaagggact tgctgttatg tcagtgaaac tttggtaaat tatcgtatcc tgtggcagga
     6961 tgatggtttg ttacagcatt gcaattcatt aaaaaggggg cttacgaaga acagtggata
     7021 tgatcgcaac taaaacgcct gccattgata aagctgcccg aatatttcat tttattgccg
     7081 ggaaaggctc tgccacctac agtgaaattc accagaatgt cggattgcct caaagcacca
     7141 cctcggccct gcttgccagc ctgaccacgc atgcctttct gagacaaaaa aatggccggt
     7201 attatctggg gctaatcttt tacgagcttg gtaataaagc cattcaggat tttgattttc
     7261 gtgaactggc tgaagagtca ctcgtttttc tccgtgacca gacccgtctt gcctgccatc
     7321 tgggcgttct ggatggttcc accggcatct atctggcgaa aaaagaaagc cagaatgcga
     7381 ttatcgttcg cagctggacg ggaaaacgac tttccctgca cagttcctcc cttgggaaag
     7441 tgttgttggc ctggcttccg gaagaccaca ttgatgctct tttacccaca gaacaccttc
     7501 ctgcgtttac tgcccggaca attacaacac gtaaagcgtt gaaagctgag ctggaggtgg
     7561 tccgcctgca gggatgggcg tttgataatc aggaagatgt cgacgatgtc acctgtattg
     7621 ccgcgccggt atttaacagt gatcaggccg ttgttgccgc aataagtatt tccggggttc
     7681 gttttcagat gcctgaggaa aaaatacctg agtatgcgaa cgcggtgatg aaagccgctg
     7741 gcatgatgtc tgaaaagtta tgcggtatat gactggtccg ctcacaggtc atgataaccc
     7801 gtgagcggcg ttcggcctta cgttgctctg acgcgggagg ctccgtgttt tgcccacaac
     7861 gcagtgatca ccggcacgac aatggcggtg atcaccacac aggatgccac cagagtggtg
     7921 gctgccggtg cggcctcagc gaagtccggc gcgatctcgg ctaccagcaa cggagtggcc
     7981 acggcagcgc cagcagtact cgatgcggca attccagccg tgccacggcc cttacccagc
     8041 atgatatcca tgattatcag tggtacgcct gtgataacaa tgactgccag agccataaga
     8101 atacccagca gtccggtgtc gatgatgacg ccaagattaa tggtattccc caacgcaaat
     8161 gcaaagaatg ggatcagact tttggtggcc ctggaaaaca attcccgaag ttcgggatca
     8221 agattaccca gcaaaaaccc caccagaaag gggatcagga cgccggcgag gtgttcaggt
     8281 tcgaacgttg cctgcccggc ggaccccaga ataatcatcg tcatcagggg gccggactca
     8341 agcgagataa gtacagaagc accggcttct tcttttgtgc cgtactcctg catcagggaa
     8401 gcataaagcc cggcattggt catatccatc gccgcaacaa tcgccagaac agataaaccg
     8461 gcaaagaaac ctgtttttac cattccccct tccggtagga gctgggcaaa aacaaaggca
     8521 cagacccagg cagtggcgat ttttgtggct accagtgtgc cagacttttt gagaacggtc
     8581 ccggttgcac tgattttaat ggaggcgccc atacagaaca gccagacagc gaggatgggg
     8641 accacgccgg tgatcatgcc attagtaaat gaaccaaagt atttgccggt acctggccag
     8701 aaggtatgca gtaccgcccc cagaaacagg ggggccagca tcattcctcc cggaattttt
     8761 tcaactgttt ttttgatatt cattatcgtc cctttttaat gtcagatacg gccagtcaga
     8821 gcaccatgat ggcttcaatt tcaataacag ccccctgcgg cagggcggcg atacccaacg
     8881 tagccctggc ggggtacggt ggcgaaaagt attccgacat aatggcgtta aatcgcggga
     8941 acatgttcac gtcggtcaga taggcattca gcttcacaat atccctatca ctccctcctg
     9001 cggcctctgc caccgacagc aaatttttaa acgcctgacg ggcctgttca tcgaaatctg
     9061 ttgagaccac ttgcatggta tgcgggtcaa aaccgacctg accggcgaga taaatggttt
     9121 ccccggcttt caccgcctga gataatgccc caccgggggc aggagccccg gtggtataaa
     9181 tgatgtcttt tttcatgatg tgcctcctgt ccttttgcgg ttacagaccg aacaatttgt
     9241 ctaggcagtc tgcctggtcg gctgttaact ggccgttatg attccgtgag accggcgtca
     9301 tgtagccata ctttctggaa atggcggttt tgatcgtact gacaaaatca tcaccaatgg
     9361 catatatccc ggaaagtcgc ccaatttctt tgtgtaatcc ggcgacttca ttgaggtcgc
     9421 cggcccgata ggcccgcata atttgggcaa aaagctccgg caccacatta ttcagtccgg
     9481 aaagaactcc ggcgcctccg ttcatcagat tgggtacaaa gtactcatca aaaccggcga
     9541 atacggcaaa atcttttctc agcggtgtga ccgcgttgac gatggcccgg gtgtgagacg
     9601 cacagtccac agtatctttg atgccaataa accgcgggca gctttccgcc agcattctga
     9661 caaggtgagc atccacatca catccggttc tggccgggaa gttatagata aaccagtcac
     9721 cggcaagttt acggtcaaga ctgcggaaat agctaagtaa ctgacgggat gtctgggcgt
     9781 agtaatactg tggcagtacc attactgcgt catagccact gtcataggcg gcatcagcca
     9841 gataaagcat gtcctcttca acggtagatg aaatatgggc aatcaaaggg aggtcactca
     9901 ttttccgggc ttccaggatg agcattgccc gttcgcggac gctcattgtg gcaaattccc
     9961 cgatggtacc tgatagcaga atgccgttga tgccggaatc agaaaggcgt gccatatgtt
    10021 tttccagccc cgtgaggtct atctgaccgc tgtcatccag aggaatgacg gaagggcaga
    10081 ctgcccctga aaacttcatt gtctgattca tattatttgt tccttttgcg tggtatcagc
    10141 gtcatgcctg ctgatacccg ccccggcctt tacggaagaa ggatggatat cagtagccca
    10201 aacaccaata tagtgattta gtaatcacta tattggtgtt tgggctgact gggaaatcat
    10261 ctttataaga actgtgcgtg agttcaagaa ttcattcgtg tcgttccggt gaactcatat
    10321 tgacaggtca attggcaact gcgtcaggtt accaagggaa gcggttgagc gtctttatag
    10381 cgacgatcgg cacgcaaggt catcataaat gccagggcgg atgctctgcc ggatggcgtt
    10441 agcaggacat ttttttcagc caagcgcgct gcggcggctt cgccaataag ctgcgtgccg
    10501 actggcagtg ggtgatcggc atcggcggcc tcggccatat cgccattaag ctgctgcggg
    10561 cgatgggcgc cgatcgcgta gtcaatagcc gcgatcctga agccttaaaa gtgctggccg
    10621 ggcagtttga cctgagcatc aacaccgtgg cggtggatct tgactggcag ccgtacttcg
    10681 aagccctgtc ctagggcggc aacttccaca ccgtcgacgc ggtgatgaag ccgttcccgg
    10741 taccggcctt tacccttatc ggcgacgact gcagcatctc cggctcggcg atcggtaacc
    10801 catccgaact gcgcacgctg aggaagttcg ccggacgcag caaagtggct ccgaccaccg
    10861 agctgttccc tatgtcgcaa attaacgaag cgttgaaaca cgttcgcgaa ggcaaagccc
    10921 gctaccgcgc ggtgctgaaa gccgact
//
LOCUS       DAMZFT010000155        10870 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.155, whole genome shotgun sequence.
ACCESSION   DAMZFT010000155 DAMZFT010000000
VERSION     DAMZFT010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10870)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10870)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10870
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.155"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(44..136)
                     /locus_tag="PN977_004285"
     tRNA            complement(44..136)
                     /locus_tag="PN977_004285"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(100..102),aa:Ser,seq:gct)
     gene            complement(452..637)
                     /gene="csrA"
                     /locus_tag="PN977_004286"
     CDS             complement(452..637)
                     /gene="csrA"
                     /locus_tag="PN977_004286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HDI6044247.1"
                     /translation="MLILTRRVGETLMIGDEVTVTVLGVKGNQVRIGVNAPKEVSVHR
                     EEIYQRIQAEKSQQSSY"
     gene            complement(872..3502)
                     /gene="alaS"
                     /locus_tag="PN977_004287"
     CDS             complement(872..3502)
                     /gene="alaS"
                     /locus_tag="PN977_004287"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HDI6044248.1"
                     /translation="MSKSTAEIRQAFLDFFHSKGHQVVASSSLVPHNDPTLLFTNAGM
                     NQFKDVFLGLDKRNYSRATTSQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDY
                     FKHDAIQFAWELLTSEKWFALPKERLWVTVYESDDEAYEIWEKEVGIPRERIIRIGDN
                     KGAPYASDNFWQMGDTGPCGPCTEIFYDHGDHIWGGPPGSPEEDGDRYIEIWNIVFMQ
                     FNRQADGTMEPLPKPSVDTGMGLERIAAVLQHVNSNYDIDLFRTLIQAVAKVTGATDL
                     SNKSLRVIADHIRSCAFLIADGVMPSNENRGYVLRRIIRRAVRHGNMLGAKETFFYKL
                     VGPLIDVMGSAGEDLKRQQAQVEQVLKTEEEQFARTLERGLALLDEELAKLSGDTLDG
                     ETAFRLYDTYGFPVDLTADVCRERNIKVDEAGFEAAMEEQRRRAREASGFGADYNAMI
                     RVDSASEFKGYDHLELNGKVTALFVDGKAVDAINAGQEAVVVLDQTPFYAESGGQVGD
                     KGELKGANFSFAVEDTQKYGQAIGHIGKLATGSLKVGDAVQADVDEARRARIRLNHSA
                     THLMHAALRQVLGTHVSQKGSLVNDKVLRFDFSHNEAMKPEEIRAVEDLVNAQIRRNL
                     PIETNIMDLEAAKAKGAMALFGEKYDERVRVLSMGDFSTELCGGTHASRTGDIGLFRI
                     ISESGTAAGVRRIEAVTGEGAIATVHADSDRLSEVAHLLKGDSNNLADKVRSVLERTR
                     QLEKELQQLKEQAAAQESANLSSKAIDVNGVKLLVSELSGVEPKMLRTMVDDLKNQLG
                     STIIVLATVAEGKVSLIAGVSKDVTDRVKAGELIGMVAQQVGGKGGGRPDMAQAGGTD
                     AAALPAALASVKGWVSAKLQ"
     gene            complement(3631..4131)
                     /gene="recX"
                     /locus_tag="PN977_004288"
     CDS             complement(3631..4131)
                     /gene="recX"
                     /locus_tag="PN977_004288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HDI6044249.1"
                     /translation="MTESTSLRPAYARLLDRAVRILAVRDHSEQELRRKLAAPIMGKN
                     GPEEIDATAEDYERVIAWCHEHGYLDDSRFVARFIASRSRKGYGPARIRQELNQKGIS
                     REATEKAMRECDIDWCALARDQATRKYGEPLPTVFSEKVKIQRFLLYRGYLMEDIQDI
                     WRNFAD"
     gene            complement(4200..5261)
                     /gene="recA"
                     /locus_tag="PN977_004289"
     CDS             complement(4200..5261)
                     /gene="recA"
                     /locus_tag="PN977_004289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HDI6044250.1"
                     /translation="MAIDENKQKALAAALGQIEKQFGKGSIMRLGEDRSMDVETISTG
                     SLSLDIALGAGGLPMGRIVEIYGPESSGKTTLTLQVIAAAQREGKTCAFIDAEHALDP
                     IYARKLGVDIDNLLCSQPDTGEQALEICDALARSGAVDVIVVDSVAALTPKAEIEGEI
                     GDSHMGLAARMMSQAMRKLAGNLKQSNTLLIFINQIRMKIGVMFGNPETTTGGNALKF
                     YASVRLDIRRIGAVKEGENVVGSETRVKVVKNKIAAPFKQAEFQILYGEGINFYGELV
                     DLGVKEKLIEKAGAWYSYKGEKIGQGKANATAWLKDNPETAKEIEKKVRELLLSNPNS
                     TPDFSVDDSEGVAETNEDF"
     gene            complement(5341..5838)
                     /gene="pncC"
                     /locus_tag="PN977_004290"
     CDS             complement(5341..5838)
                     /gene="pncC"
                     /locus_tag="PN977_004290"
                     /EC_number="3.5.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide-nucleotide amidase"
                     /protein_id="HDI6044251.1"
                     /translation="MTDSELMQLSEQVGQALKARGATVTTAESCTGGWVAKVITDIAG
                     SSAWFERGFVTYSNEAKAQMIGVREETLAQHGAVSEPVVVEMAIGALKAARADYAVSI
                     SGIAGPDGGSEEKPVGTVWFAFATARGEGITRRECFSGDRDAVRRQATAYALQTLWQQ
                     FLQNT"
     gene            complement(5983..7068)
                     /gene="mltB"
                     /locus_tag="PN977_004291"
     CDS             complement(5983..7068)
                     /gene="mltB"
                     /locus_tag="PN977_004291"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase B"
                     /protein_id="HDI6044252.1"
                     /translation="MFKRRYVTLLPLFVLLAACSSKPKPTETETTTGTPSGGFLLEPQ
                     HNVMQMGGDFANNPNAQQFIDKMVNKHGFNRQQLQEILSQAKRLDSVLRLMDNQAPTT
                     SVKPPSGPNGAWLRYRKKFITPDNVQNGVVFWNQYEDALNRAWQVYGVPPEIIVGIIG
                     VETRWGRVMGKTRILDALATLSFNYPRRAEYFSGELETFLLMARDEQDDPLNLKGSFA
                     GAMGYGQFMPSSYKQYAVDFSGDGHINLWDPVDAIGSVANYFKAHGWVKGDQVAVMAN
                     GQAPGLPNGFKTKYSISQLAAAGLTPQQPLGNHQQASLLRLDVGTGYQYWYGLPNFYT
                     ITRYNHSTHYAMAVWQLGQAVALARVQ"
     gene            7325..7888
                     /gene="srlA"
                     /locus_tag="PN977_004292"
                     /gene_synonym="gutA"
     CDS             7325..7888
                     /gene="srlA"
                     /locus_tag="PN977_004292"
                     /gene_synonym="gutA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HDI6044253.1"
                     /translation="MIETITHGAEWFIGLFQKGGEVFTGMVTGILPLLISLLVIMNAL
                     INFIGQHRIERFAQRCAGNPVSRYLLLPCIGTFVFCNPMTLSLGRFMPEKYKPSYYAA
                     ASYSCHSMNGLFPHINPGELFVYLGIASGLTTLNLPLGPLAVSYLLVGLVTNFFRGWV
                     TDLTTAIFEKKMGIQLEQKVHLAGATS"
     gene            7885..8844
                     /gene="srlE"
                     /locus_tag="PN977_004293"
                     /gene_synonym="gutE"
     CDS             7885..8844
                     /gene="srlE"
                     /locus_tag="PN977_004293"
                     /gene_synonym="gutE"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HDI6044254.1"
                     /translation="MTRIRIEKGTGGWGGPLELEAKPGKKIVYITAGTRPAIVDKLAQ
                     LTGWQAIDGFKEGEPAEAEIGVAVIDCGGTLRCGIYPKRRIPTINIHSTGKSGPLAQY
                     IVEDIYVSGVKEENITVVGDATPQPSSVGRDYDTSKKITEQSDGLLAKVGMGMGSAVA
                     VLFQSGRDTIDTVLKTILPFMAFVSALIGIIMASGLGDWIAHGLAPLASHPLGLVMLA
                     LICSFPLLSPFLGPGAVIAQVIGVLIGVQIGLGNIPPHLALPALFAINAQAACDFIPV
                     GLSLAEARQDTVRVGVPSVLVSRFLTGAPTVLIAWFVSGFIYQ"
     gene            8855..9226
                     /gene="srlB"
                     /locus_tag="PN977_004294"
                     /gene_synonym="gutB"
     CDS             8855..9226
                     /gene="srlB"
                     /locus_tag="PN977_004294"
                     /gene_synonym="gutB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HDI6044255.1"
                     /translation="MTVIYQTTITRIGASATDALSDQMLITFREGAPADLEEYCFIHC
                     HGELKGALHPGLQFTLGRHRYPVTAVGSVAEDNLRELGHVTLRFDGLSEAEFPGTVHV
                     AGPVPDDIAPGSVLKFESVKE"
     gene            9230..10009
                     /gene="srlD"
                     /locus_tag="PN977_004295"
     CDS             9230..10009
                     /gene="srlD"
                     /locus_tag="PN977_004295"
                     /EC_number="1.1.1.140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sorbitol-6-phosphate dehydrogenase"
                     /protein_id="HDI6044256.1"
                     /translation="MNQVAVVIGGGQTLGAFLCHGLAAEGYRVAVVDIQSDKAANVAQ
                     EINAEYGEGMAYGFGADATSEQSVLALSRGVDEIFGRVDLLVYSAGIAKAAFISDFQL
                     GDFDRSLQVNLVGYFLCAREFSRLMIRDGIQGRIIQINSKSGKVGSKHNSGYSAAKFG
                     GVGLTQSLALDLAEYGITVHSLMLGNLLKSPMFQSLLPQYATKLGIKPEQVEQYYIDK
                     VPLKRGCDYQDVLNMLLFYASPKASYCTGQSINVTGGQVMF"
     gene            10115..10474
                     /gene="gutM"
                     /locus_tag="PN977_004296"
     CDS             10115..10474
                     /gene="gutM"
                     /locus_tag="PN977_004296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
                     /protein_id="HDI6044257.1"
                     /translation="MVSALITVAVIAWCAQLALGGWQISRFNRAFDTLCQQGRVGVGR
                     SSGRFKPRVVVAIALDDQQRIVDTLFMKGLTVFARPQKIPAITGMHAGDLQPDVIFPH
                     DPLSQNALSLALKLKRG"
     gene            10541..>10870
                     /gene="srlR"
                     /locus_tag="PN977_004297"
                     /gene_synonym="gutR"
     CDS             10541..>10870
                     /gene="srlR"
                     /locus_tag="PN977_004297"
                     /gene_synonym="gutR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucitol operon DNA-binding transcriptional
                     repressor SrlR"
                     /protein_id="HDI6044258.1"
                     /translation="MKPRQRQAAILEYLQKQGKCSVEELAQYFDTTGTTIRKDLVILE
                     HAGTVIRTYGGVVLNKEESDPPIDHKTLINTHKKELIAEAAVSFIHDGDSIILDAGST
                     VLQMVPLL"
BASE COUNT         2519 a         2952 c         2686 g         2713 t
ORIGIN      
        1 gttcgtagcc gagtactcta tccagctgag ctacggatgc aaatggcggt gaggcgggga
       61 ttcgaacccc ggatgcagct tttgaccgca tactccctta gcaggggagc gccttcagcc
      121 tctcggccac ctcaccacac gcctcttacg agtgcttcga agaacttgtt tatcgctcat
      181 cgtcgctgcg tggcgcacat attactttct gagacttata agtcaaacaa tttttcccac
      241 acttttatcg tttgcacact tcacgctcaa ttagtctgta aaaacgtcaa aaagagtgtt
      301 ttatcaacag aaaaacggag gtctgacaga tagcagtaat gcaaaaaaat ggagacttaa
      361 gttgaatgaa cgggagtaaa gcgaaaagac aatggagtga aagagaaatt ttgagggtgc
      421 gtctcaccga taaagatgag acgcggaaag attagtaact ggactgctgg gatttttcag
      481 cctggatacg ctggtagatc tcttcacggt gaacagaaac ttccttcggg gcatttacgc
      541 caatacgtac ctggttgccc tttaccccta aaactgtcac ggtgacctca tccccaatca
      601 tgagggtctc accaactcga cgagtcagaa tcagcattct ttgctccttg aaagattaaa
      661 agagtcgggt ctctctgtat cccggcatta tccatcatat aacgccaaaa agtaagcaat
      721 gacaaacaca ttacatctaa gcagtcatgg cattacattc tgttaaacct aagtttagcc
      781 gatatacaca acttcaacct gactttatcg ttgtcgatag cgttaatgcg aatgccgtga
      841 agcgagtcca cggcattgcc tgacgcttat attattgcaa tttcgcgctg acccagcctt
      901 tcacactggc taacgctgca ggtaaggccg cagcatccgt accaccggct tgcgccatgt
      961 caggacgtcc accacccttg ccgcccacct gctgagcgac cataccaatc agctcccctg
     1021 ctttcacacg atctgtgacg tccttagata cgcctgcaat cagagaaacc ttaccttcgg
     1081 ctaccgttgc cagcacgata attgtcgacc ccagctgatt ttttaaatcg tcaaccatgg
     1141 tacgcaacat tttcggctca acaccgctaa gctcgctaac caacagctta acaccattaa
     1201 catcaattgc cttactggaa agatttgcgc tctcctgtgc ggcagcttgt tctttaagct
     1261 gttgtaactc tttttccaac tgacgcgtac gttccagtac tgaacgcact ttatcagcca
     1321 gattattgct atcgcctttc agcagatgcg cgacttcgct taagcgatca ctgtctgcat
     1381 gaacggtggc gatagcgcct tctccggtta ccgcttcgat acgacgaacg cctgcagcag
     1441 tacccgattc agagatgatg cggaacagac caatatcacc agtgcggctg gcgtgagtac
     1501 cgccacacaa ctcggtggag aaatcaccca tgctcagcac gcgtacgcgc tcatcatact
     1561 tctcgccaaa cagcgccatc gcacctttcg ctttcgccgc ttcgagatcc atgatgttgg
     1621 tttcgatcgg caagttacgg cgaatctgtg cgttcaccag gtcttcgacc gcacgaatct
     1681 cttctggttt catcgcttcg ttgtgtgaga agtcgaagcg cagcactttg tcgttaacca
     1741 gtgaaccttt ctgcgataca tgagtaccca gaacctggcg cagcgcagcg tgcatcaggt
     1801 gcgttgcgga gtgattcaga cgaatacggg cgcgacgagc ctcatcaaca tcagcctgca
     1861 ccgcgtcgcc cactttcaga gaacccgtag caagtttacc gatgtgacca atcgcctggc
     1921 cgtatttctg agtatcttcc accgcaaagg agaagttagc gcctttcagt tcgcctttat
     1981 caccaacctg accgccggat tccgcataga atggcgtttg atccagcacg accacagctt
     2041 cctggcctgc attgatggca tcaaccgctt taccatcaac aaacagcgcg gtcactttgc
     2101 cgttcagttc cagatggtca tagcctttaa attcagatgc actgtcaaca cggatcattg
     2161 cgttgtaatc ggcaccaaag ccgctggctt cgcgcgcacg acgacgctgc tcttccattg
     2221 ctgcttcaaa tccagcttcg tcaactttga tgttgcgctc acgacaaaca tcagccgtca
     2281 ggtcaaccgg gaagccatag gtgtcgtaca gacggaaagc agtttcacca tccagcgtat
     2341 caccagaaag ttttgccagc tcttcatcca gcaacgccag accgcgctcc agagtacgag
     2401 caaactgctc ttcttcagtc ttcagcacct gctcaacctg cgcctgctgg cgtttcaggt
     2461 cttcacccgc agagcccata acgtcgatca gcggaccaac cagtttgtag aaaaaggttt
     2521 ctttcgcgcc gagcatatta ccgtgacgca ctgcgcgacg aatgatacga cgcagtacat
     2581 aaccacggtt ttcgttggac ggcattacgc catccgcgat caggaacgca caagaacgaa
     2641 tgtggtcagc gattacgcgc agcgatttat tgctcagatc ggttgcgcca gtgactttcg
     2701 ctaccgcctg gatcagcgtg cggaacaagt cgatgtcata gttagagtta acgtgttgca
     2761 gcaccgcagc aatacgctcc agacccatac cggtatctac agacggcttc ggcagcggtt
     2821 ccatcgtgcc atcggcctgg cggttgaact gcatgaagac gatgttccag atctcaatgt
     2881 agcggtcgcc gtcttcttcc ggacttcccg gagggccacc ccaaatgtgg tcgccgtgat
     2941 cgtagaagat ttcggtgcac gggccgcacg gaccagtgtc acccatctgc cagaagttgt
     3001 cagatgcgta tggcgcacct ttgttatcgc cgatgcgaat aatacgttcg cgcgggatcc
     3061 ctacttcttt ttcccagatt tcgtaggctt cgtcgtcgct ttcatagacg gtaacccaca
     3121 gacgctcttt cggcagggca aaccattttt cgctggtcag cagttcccat gcaaactgaa
     3181 tggcatcgtg tttgaaatag tcgccgaagc tgaagttgcc cagcatttcg aagaaggtat
     3241 ggtgacgcgc ggtgtaaccg acgttttcca ggtcgttgtg tttaccaccc gcacgcacgc
     3301 agcgttggga agtggtagcg cgggaataat tacgcttgtc gagcccaagg aacacatcct
     3361 tgaactggtt catcccggcg ttggtaaaca acaaagttgg gtcgttatgg ggtaccaggg
     3421 agctgctggc aactacctga tgtcccttac tatggaaaaa gtcgagaaac gcctgacgga
     3481 tctcagcggt gctcttgctc ataattatcc tgaaatcaag ctaacgaaat atcgccacca
     3541 gccgttgcgt gtcgttaacc gcccggctgg taactgaaaa gtgggaataa gataagtttt
     3601 cttgactggg aagtaaaata ccgtatgcgt tcagtcggca aaatttcgcc aaatatcctg
     3661 gatatcttcc atcagatagc cacgatagag cagaaaacgc tggatcttaa ctttttctga
     3721 aaagacagtt ggcaaaggtt cgccatattt tcgcgtcgcc tgatcgcgcg ccagtgcgca
     3781 ccagtcgatg tcacattcac gcatcgcttt ttctgtcgct tcgcgggaaa tacctttctg
     3841 attcagttcc tgacgaatac gcgcaggtcc ataacctttg cggctacggc tggcgataaa
     3901 gcgcgcaaca aatcggctgt catcaagata gccatgttca tggcaccagg caataacgcg
     3961 ctcgtaatct tctgccgtgg catcaatctc ttccgggcca tttttgccca taatcggtgc
     4021 cgcgagttta cgtcgcagtt cttgctcact gtgatcgcgc accgccagaa tgcgtaccgc
     4081 acgatccaac aggcgagcat atgccgggcg aagggatgtt gattctgtca tggcatatcc
     4141 ttacaactta aaaaagcaaa agggccgcag atgcgaccct tgtgtatcaa acaagacgat
     4201 taaaaatctt cgttagtttc tgctacgcct tcgctgtcat ctacagagaa atccggcgtt
     4261 gagttcgggt tgctcagcag caactcacgt actttcttct cgatctcttt cgcggtttcc
     4321 gggttatctt tcagccaggc agtcgcattc gctttaccct gaccgatctt ctcacctttg
     4381 tagctgtacc atgcgcctgc tttctcgatc agcttctctt tcacgcccag atcaaccagt
     4441 tcgccataga agttgatacc ttcgccgtag aggatctgga attcagcctg tttaaacggc
     4501 gcagcgattt tgttcttcac cactttcaca cgggtttcgc tacccaccac gttttcgccc
     4561 tctttcaccg cgccgatacg acggatgtcg agacgaacag aggcgtagaa tttcagcgcg
     4621 ttaccaccgg tagtggtttc cgggttaccg aacatcacac caattttcat acggatctgg
     4681 ttgatgaaga tcagcagcgt gttggactgc ttcaggttac ccgccagctt acgcatcgcc
     4741 tggctcatca tacgtgccgc aaggcccatg tgagagtcgc cgatttcgcc ttcgatttcc
     4801 gctttcggcg tcagtgccgc cacggagtca acgacgataa cgtctactgc gccagaacgt
     4861 gccagggcgt cacagatttc cagtgcctgc tcgccagtgt ccggctggga gcacagcagg
     4921 ttgtcgatat cgacgcccag tttacgtgcg tagattgggt ccagcgcgtg ttcagcatcg
     4981 ataaacgcac aagttttacc ttcgcgctgg gctgcggcga tcacctgcaa cgtcagcgtg
     5041 gttttaccgg aagattccgg tccgtagatt tcgacgatac ggcccatcgg cagaccacct
     5101 gccccaagcg cgatatccag tgaaagcgaa ccggtagaga tggtttccac atccatggaa
     5161 cggtcttcac ccaggcgcat gatggagcct ttaccaaatt gtttctcaat ctggcccagt
     5221 gctgccgcca acgctttctg tttgttttcg tcgatagcca tttttactcc tgtcatgccg
     5281 ggtaataccg gatagtcaat atgttctgtt gaagcaatta tactgtatgc tcatacagta
     5341 tcaagtgttt tgtagaaatt gttgccacaa ggtctgcaat gcatacgcag tagcctgacg
     5401 acgcaccgca tcacggtcac cgctgaagca ttcccggcgg gtaatacctt cgccgcgggc
     5461 ggtggcaaaa gcaaaccaga cggtgccgac aggcttctct tcactgccgc catccggccc
     5521 ggcgatacca ctaatagaca cggcataatc agcacgagcc gctttcagtg cgcctatcgc
     5581 catttccacc acgacgggtt cactcaccgc gccatgctgc gccagcgtct cttcacgtac
     5641 gccgatcatt tgcgctttgg cttcgttact gtaggtgaca aatccgcgtt caaaccaggc
     5701 ggagctaccg gcaatatcgg taatcacttt cgctacccaa ccaccggtac aagactcagc
     5761 agttgttacg gttgcgccac gggctttcag cgcctgccca acctgttcgc ttaactgcat
     5821 cagttcactg tcagtcatca caacctctgt cagtcaaaaa tttatgccgg acaagatagc
     5881 actttcgtca ggaagatggt gttggcgttg caattttacg aggaggattc agaaaaaagc
     5941 tgattagcca gagggaagct cacgcccccc tcttgcaaag agttactgca cgcgcgccag
     6001 cgccacggct tgtcctaact gccagaccgc cattgcgtaa tgggtgctgt ggttataacg
     6061 ggtaatggtg tagaagttcg gcagaccgta ccagtactgg taacctgttc caacatccag
     6121 acgcagcagg ctggcttgtt gatggttgcc cagcggctgc tgtggcgtta aacctgccgc
     6181 ggcaagctgc gaaatgctgt acttggtttt gaagccattt ggcaagcctg gagcctgacc
     6241 gtttgccatt accgcaacct gatcaccttt cacccagccg tgcgctttga aatagttcgc
     6301 cacactacca atggcatcaa ccggatccca caggttgata tgcccgtcgc cgctgaaatc
     6361 taccgcatat tgtttgtaag acgacggcat aaactgtccg tagcccatcg ccccggcaaa
     6421 ggaacctttc agattgagcg gatcgtcctg ctcgtcgcgc gccattaaca ggaaggtttc
     6481 cagctcgcca gagaaatatt ccgcgcggcg tgggtagtta aatgacagcg ttgccagcgc
     6541 atcgaggata cgggttttcc ccatcacgcg cccccaacgg gtttcaacgc cgataatccc
     6601 gacgataatt tccggcggta cgccatacac ctgccacgcg cgattcaacg catcttcata
     6661 ctgattccag aaaaccacac cattctgcac gttgtccggc gtaataaatt ttttgcgata
     6721 acgcagccat gcgccgttcg gacctgatgg cggtttcacc gatgtggttg gtgcctggtt
     6781 atccatcagc cgcagtaccg aatccagacg tttcgcctgg gagagaattt cctgcaactg
     6841 ctgacgattg aaaccgtgtt tgttcaccat tttgtcgatg aactgctggg cattcgggtt
     6901 attggcgaag tcaccgccca tctgcatcac attgtgctgc ggctcaagca ggaagccgcc
     6961 agacggcgtt ccggtggtcg tttcagtctc agtaggtttt ggcttgctgc tacaggcggc
     7021 aagcaacaca aaaaggggaa gcaatgttac ataacgacgc ttgaacatga ggggtccatt
     7081 taacagattc aaccaggggc aagtatggta aagcatcccg ccccgcacaa ggaagcggta
     7141 gtcactgccc gatacggact ttacataact caactcattc tcctcactac cttctttata
     7201 taaactttca aaataaaata tttatctttc attttgcgat caaaataaca cttttaaatc
     7261 tttcaatctg attagattag attgccgttt ggtaataaaa caataaatcc tgaaggagag
     7321 aacaatgata gaaaccatta ctcatggtgc agagtggttt atcgggctgt tccaaaaggg
     7381 cggagaggtg tttaccggga tggtgaccgg cattcttccg ctgttgatta gcctgttggt
     7441 tatcatgaac gcactgatta attttatcgg tcagcatcgt attgaacgtt ttgctcaacg
     7501 ttgcgccggt aaccctgttt cccgttacct gctgttaccg tgcattggca cgtttgtctt
     7561 ttgcaatccg atgaccttaa gcctgggtcg ctttatgccg gaaaagtaca aacccagcta
     7621 ttacgcggcg gcctcttata gctgccactc aatgaatggc ctcttccccc atatcaaccc
     7681 tggcgaactg tttgtttatc ttggcattgc cagcggtctg acaacactga atctgccact
     7741 tggcccactg gcggtgagtt atctgctggt tggtttggtc accaacttct tccgcggctg
     7801 ggtgaccgat ctgaccaccg ccatttttga gaaaaagatg ggcattcaac ttgaacaaaa
     7861 agttcatctg gcaggagcaa catcatgacg cgtattcgga tcgaaaaagg aacgggtggc
     7921 tggggcggcc cgcttgagct ggaagccaaa ccgggaaaaa aaatcgtcta tatcaccgcc
     7981 ggtacccggc ctgcgattgt tgacaaactg gcacagctta ctggctggca ggctattgac
     8041 ggatttaaag aaggtgaacc cgcggaggcg gaaattggtg tcgcggtaat cgactgtggc
     8101 ggcacattac gctgtggcat ctatccgaaa cggcgtattc ccaccattaa tatccactcg
     8161 acgggcaagt ccggcccgct ggcgcagtac attgtggaag atatttatgt ctctggcgta
     8221 aaagaagaaa acatcactgt agtgggtgat gcgacaccac aaccctcttc cgtgggccgt
     8281 gactatgaca ccagcaagaa aatcaccgaa caaagcgatg gtttactggc gaaggtggga
     8341 atgggtatgg gttctgccgt tgccgtgttg tttcaatctg gtcgtgacac catcgacact
     8401 gtattaaaaa ccattctgcc gtttatggcg ttcgtctcgg cgcttatcgg catcattatg
     8461 gcttctggcc ttggtgactg gattgcccac ggtcttgctc cgctggcgag ccatccactg
     8521 ggtctggtca tgttggcgct catctgctcc ttcccgctgc tttcaccttt cctcggccca
     8581 ggcgcagtta tcgcgcaggt tatcggcgta ttgattggtg tgcagattgg tctcggcaat
     8641 attccgccgc atctggcttt acctgcactg tttgccatca acgcgcaggc ggcctgcgac
     8701 ttcatcccgg tcggtttgtc gctggcggaa gctcgtcagg acacggttcg cgtcggtgtc
     8761 ccttctgttc tggtgagccg ctttttaacc ggcgcgccaa ctgtactgat cgcctggttt
     8821 gtctccggtt ttatctatca atagaggctg aaacatgacc gttatttatc agaccaccat
     8881 cacccgtatt ggcgcgagcg ccactgacgc cctcagcgac cagatgctca tcacctttcg
     8941 tgaaggcgcg cctgcggatc tcgaagagta ttgcttcatt cattgccacg gcgagttgaa
     9001 aggtgcactc catcccggtt tgcaatttac gctcgggcgg catcgctatc cggtgaccgc
     9061 cgttggcagc gtggcggaag acaaccttcg cgaactgggt catgtcaccc tgcgcttcga
     9121 tggtttaagc gaagcggaat ttccgggcac tgtccatgtg gcaggccctg tacccgacga
     9181 tatcgcgccg ggatcggttt tgaagtttga atctgttaag gagtaaaaaa tgaatcaggt
     9241 tgccgttgtc atcggtggtg ggcaaacatt aggcgcgttc ctgtgccacg gtctggctgc
     9301 cgaggggtat cgcgtcgcgg ttgtcgatat tcagagcgac aaagccgcaa atgtggcaca
     9361 agaaattaac gccgaatatg gtgaaggtat ggcgtacggt tttggtgctg acgccaccag
     9421 cgagcaaagc gttctggcgc tttctcgtgg ggtagatgaa atctttggtc gcgtggattt
     9481 actggtctac agcgccggaa tagcaaaagc agcctttatc agcgatttcc agcttggaga
     9541 ttttgaccgc tcgctacagg tgaatctggt gggctatttc ctgtgtgccc gtgaattttc
     9601 gcgtttgatg atccgcgacg ggattcaggg tcgcattatt cagatcaact cgaaatccgg
     9661 caaagtgggc agcaaacaca actctggcta cagcgcagcg aaatttggtg gcgtcgggct
     9721 gactcaatca ctggcgctgg atctggcgga gtacggcatt acggtgcatt cgctgatgct
     9781 cggtaacctg ctgaaatcgc ctatgttcca gtcactgttg ccacaatacg cgaccaagct
     9841 gggtatcaaa ccggagcaag tcgagcagta ttacatcgat aaagtaccgc tcaaacgcgg
     9901 ctgcgattat caggatgtgc tgaatatgct gctgttctac gccagtccta aggcgtcgta
     9961 ctgcactggt cagtcgatca atgtcaccgg cggtcaggtg atgttctgat caacagcgga
    10021 gatcttttaa ggatctccgc gcgactaata gaatgcctga tgcgctacgc ttatcaggcc
    10081 tacaggactt ccgccactac attacggaga agttatggta tccgcactca tcaccgtcgc
    10141 cgttatcgcc tggtgtgcgc aactggcttt aggcggctgg caaatttctc gttttaaccg
    10201 tgccttcgac acactatgcc agcaagggcg ggttggcgtg ggccgttcca gcgggcgctt
    10261 taaaccgcgg gtcgtggtcg ccatcgccct ggacgatcag cagcgcatcg tcgacacctt
    10321 gtttatgaaa ggactgaccg tcttcgcccg accgcaaaaa attcccgcaa ttaccggtat
    10381 gcatgcgggt gatttacagc ccgatgtgat ctttccccat gatccactat cacagaatgc
    10441 tctatcattg gcgcttaaac tgaaacgtgg ataatttcgt tgtgaatgtt acttgcttgc
    10501 gaagttatca ttttgaaacc taaatcaggt aatcacgccc atgaaacctc gtcagcgtca
    10561 ggccgccatt ctggagtatc tgcaaaagca gggtaaatgc tcggttgaag aattggcgca
    10621 atactttgac accacaggaa caaccattcg caaagatctg gtcattctgg aacatgccgg
    10681 aaccgtcatt cgtacttatg gcggagtggt gttgaataaa gaggaatccg atccgcctat
    10741 cgatcataaa acactcatca acacccacaa gaaagagctg attgcagaag ctgccgttag
    10801 ttttatccat gatggcgatt cgatcattct tgatgctggc agtaccgttt tgcagatggt
    10861 tcccctgctc
//
LOCUS       DAMZFT010000156        10869 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.156, whole genome shotgun sequence.
ACCESSION   DAMZFT010000156 DAMZFT010000000
VERSION     DAMZFT010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10869)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10869)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10869
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.156"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(<1..704)
                     /gene="hyi"
                     /locus_tag="PN977_004298"
     CDS             complement(<1..704)
                     /gene="hyi"
                     /locus_tag="PN977_004298"
                     /EC_number="5.3.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxypyruvate isomerase"
                     /protein_id="HDI6044259.1"
                     /translation="MLRFSANLSMLFGEYDFLARFEKAAQCGFRGVEFMFPYDYDIEE
                     LKQVLASNKLEHTLHNLPAGDWAAGERGIACIPGREEEFRDGVAAAIRYARALGNKKI
                     NCLVGKTPAGFSSEQIHATLVENLRYAANMLMKEDILLLIEPINHFDIPGFHLTGTRQ
                     ALKLIDDVGCCNLKIQYDIYHMQRMEGELTNTMTQWADKIGHLQIADNPHRGEPGTGE
                     INYEYLFTVIENSDYNG"
     gene            complement(717..2498)
                     /gene="gcl"
                     /locus_tag="PN977_004299"
     CDS             complement(717..2498)
                     /gene="gcl"
                     /locus_tag="PN977_004299"
                     /EC_number="4.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate carboligase"
                     /protein_id="HDI6044260.1"
                     /translation="MAKMRAVDAAMYVLEKEGIITAFGVPGAAINPFYSAMRKHGGIR
                     HILARHVEGASHMAEGYTRATAGNIGVCLGTSGPAGTDMITALYSASADSIPILCITG
                     QVPRARLHKEDFQAVDIEAIAKPVSKMAVTVREAALVPRVLQQAFHLMRSGRPGPVLV
                     DLPFDVQVAEIEFDPDMYEPLPVYKPAASRMQIEKAVEMLIQAERPVIVAGGGVINAD
                     AAALLQQFAELTSVPVIPTLMGWGCIPDDHELMAGMVGLQTAHRYGNATLLASDMVFG
                     IGNRFANRHTGSVEKYTEGRKIVHIDIEPTQIGRVLCPDLGIVSDAKAALTLLVEVAQ
                     EMQKAGRLPCRKEWVADCQQRKRTLLRKTHFDNVPVKPQRVYEEMNKAFGRDVCYVTT
                     IGLSQIAAAQMLHVFKDRHWINCGQAGPLGWTIPAALGVCAADPERNVVAISGDFDFQ
                     FLIEELAVGAQFNIPYIHVLVNNAYLGLIRQSQRAFDMDYCVQLAFENINSSEVNGYG
                     VDHVKVAEGLGCKAIRVFKPEDIAPAFEQAKVLIAQYRVPVVVEVILERVTNISMGSE
                     LDNVMEFEDIADNAADAPTETCFMHYE"
     gene            complement(2588..3403)
                     /gene="allR"
                     /locus_tag="PN977_004300"
     CDS             complement(2588..3403)
                     /gene="allR"
                     /locus_tag="PN977_004300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor AllR"
                     /protein_id="HDI6044261.1"
                     /translation="MTEVRRRGRPGQAEPVAQKGAQALERGIAILQYLEKSGGSSSVS
                     DISLNLDLPLSTTFRLLKVLQAADFVYQDSQLGWWHIGLGVFNVGAAYIHNRDVLSVA
                     GPFMRRLMLLSGETVNVAIRNGNEAVLIGQLECKSMVRMCAPLGSRLPLHASGAGKAL
                     LYPLAEEELMSIILQTGLQQFTPTTLVDMPTLLKDLEQARELGYTVDKEEHVVGLNCI
                     ASAIYDDVGSVVAAISISGPSSRLTEDRFVSQGELVRDTARDISTALGLKAHP"
     gene            complement(3481..3963)
                     /gene="allA"
                     /locus_tag="PN977_004301"
     CDS             complement(3481..3963)
                     /gene="allA"
                     /locus_tag="PN977_004301"
                     /EC_number="4.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ureidoglycolate lyase"
                     /protein_id="HDI6044262.1"
                     /translation="MKLQVLPLSQEAFSAYGDVIETQQRDFFHINNGLVERYHDLALV
                     EILEQDRTLISINRAQPANLPLTLHELERHPLGTQAFIPMKGEVFVVVVALGDDKPDL
                     STLRAFITNGEQGVNYHRNVWHHPLFAWQRVTDFLTIDRGGSDNCDVESIPEQELCFA
                     "
     gene            4193..5119
                     /gene="allS"
                     /locus_tag="PN977_004302"
     CDS             4193..5119
                     /gene="allS"
                     /locus_tag="PN977_004302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AllS"
                     /protein_id="HDI6044263.1"
                     /translation="MFDPETLRTFIAVAETGSFSKAAERLCKTTATISYRIKLLEENT
                     GVALFFRTTRSVTLTAAGEHLLSQARDWLSWLESMPSELQQVNDGVERQVNIVINNLL
                     YNPQAVAQLLAWLNERYPFTQFHISRQIYMGVWDSLLYEGFSLAIGVTGTEALANTFS
                     LDPLGSVQWRFVMAADHPLANVEEPLTEAQLRRFPAVNIEDSARTLTKRVAWRLPGQK
                     EIIVPDMETKIAAHLAGVGIGFLPKSLCQSMIDNQQLVSRVIPTMRPPSPLSLAWRKF
                     GSGKAIEDIVTLFTQRRPEISGFLEIFGNPRS"
     gene            5188..6282
                     /gene="mnmH"
                     /locus_tag="PN977_004303"
     CDS             5188..6282
                     /gene="mnmH"
                     /locus_tag="PN977_004303"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HDI6044264.1"
                     /translation="MQERHTEQDYRALLIADTPIIDVRAPIEFEQGAMPAAINLPLMN
                     NDERAAVGTCYKQQGSDAALALGHKLVAGEIRQQRMDAWRAACLQNPQGILCCARGGQ
                     RSHIVQSWLHAAGIDYPLVEGGYKALRQTAIQATIELAQKPIVLIGGCTGSGKTLLVQ
                     QQPNGVDLEGLARHRGSAFGRTLQPQLSQASFENLLAAEMLKTDARQNLRLWVLEDES
                     RMIGSNHLPECLRERMTQAAIAVVEDPFEIRLERLNEEYFLRMHHDFTHAYGDEQGWQ
                     EYCEYLHHGLSAIKRRLGLQRYNELAARLDAALTTQLTTGSTDGHLAWLVPLLEEYYD
                     PMYRYQLEKKAEKVVFRGEWAEVAEWVKSR"
     gene            complement(6413..8827)
                     /locus_tag="PN977_004304"
     CDS             complement(6413..8827)
                     /locus_tag="PN977_004304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDI6044265.1"
                     /translation="MIARWFWREWRSPSLLIVWLALSLAVACVLALGNISDRMEKGLS
                     QQSREFMAGDRALRSSREVPQAWLEEAQKRGLKVGKQLTFATMTFAGDTPQLANVKAV
                     DDIYPMYGDLQTNPPGLKPQAGSVLLAPRLMALLNLKTGDTIDVGDATLRIAGEVIQE
                     PDSGFNPFQMAPRLMMNLADVDKTGAVQPGSRVTWRYKFGGNENQLDGYEKWLLPQLK
                     PEQRWYGLEQDEGALGRSMERSQQFLLLSALLTLLLAVAAVAVAMNHYCRSRYDLVAI
                     LKTLGAGRAQLRKLIVGQWLMVLTLSAVTGGAIGLLFENVLMVLLKPVLPAALPPASL
                     WPWLWALGTMTVISLLVGLRPYRLLLATQPLRVLRNDVVANVWPLKFYLPIVSVVVVL
                     LLAGLMGGSMLLWAVLAGAVVLALLCGVLGWMLLNVLRRMTLKSLPLRLAVSRLLRQP
                     WSTLSQLSAFSLSFMLLALLLVLRGDLLDRWQQQLPPESPNYFLINIATEQVAPLKAF
                     LAEHQIVPESFYPVVRARLTAINGKPTEGNEDEALNRELNLTWQNTRPDHNPIVAGNW
                     PPKADEVSMEEGLAKRLNVALGDTVTFMGDTQEFRAKVTSLRKVDWESLRPNFYFIFP
                     EGALDGQPQSWLTSFRWENGNGMLTQLNRQFPTISLLDIGAILKQVGQVLEQVSRALE
                     VMVVLVTACGMLLLLAQVQVGMRQRHQELVVWRTLGAGKKLLRTTLWCEFAMLGFVSG
                     LVAAIGAETALAVLQAKVFDFPWEPDWRLWIVLPCSGALLLSLCGGWLGARLVKGKAL
                     FRQFAG"
     gene            complement(8824..9510)
                     /locus_tag="PN977_004305"
     CDS             complement(8824..9510)
                     /locus_tag="PN977_004305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001110574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDI6044266.1"
                     /translation="MPAENIVEVHHLKKSVGQGEHELSILTGVELVVKRGETIALVGE
                     SGSGKSTLLAILAGLDDGSSGEVSLVGQPLHNMDEEARAKLRAKHVGFVFQSFMLIPT
                     LNALENVELPALLRGESSAESRNGAKALLEQLGLGKRLDHLPAQLSGGEQQRVALARA
                     FNGRPDVLFADEPTGNLDRQTGDKIADLLFSLNREHGTTLIMVTHDLQLAARCDRCLR
                     LVNGQLQEET"
     gene            9481..10104
                     /gene="tesA"
                     /locus_tag="PN977_004306"
     CDS             9481..10104
                     /gene="tesA"
                     /locus_tag="PN977_004306"
                     /EC_number="3.1.1.5"
                     /EC_number="3.1.2.14"
                     /EC_number="3.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional acyl-CoA thioesterase I/protease
                     I/lysophospholipase L1"
                     /protein_id="HDI6044267.1"
                     /translation="MNFNNVFRWHLPFLFLVLLTFRAAAADTLLILGDSLSAGYRMSA
                     SAAWPALLNDKWQSKTSVVNASISGDTSQQGLARLPALLKQHQPRWVLVELGGNDGLR
                     GFQPQQTEQTLRQILQDVKAANAEPLLMQIRLPANYGRRYNEAFSAIYPKLAKEFDVP
                     LLPFFMEEVYLKPQWMQDDGIHPNRDAQPFIADWMAKQLQPLVNHDS"
     gene            10094..>10869
                     /gene="ybbO"
                     /locus_tag="PN977_004307"
     CDS             10094..>10869
                     /gene="ybbO"
                     /locus_tag="PN977_004307"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HDI6044268.1"
                     /translation="MTHKATEILTGKVMQKSVLITGCSSGIGLESALELKRQGFHVLA
                     GCRKADDVERMNSMGFTGVLIDLDSPESVDRAADEVIALTDNCLYGIFNNAGFGMYGP
                     LSTISRVQMEQQFSANFFGAHQLTMRLLPVMLPHGEGRIVMTSSVMGLISTPGRGAYA
                     ASKYALEAWSDALRMELRHSGIKVSLIEPGPIRTRFTDNVNQTQSDKPVENPGIAARF
                     TLGPEAVVDKVRHAFISEKPKMRYPVTLVTWAVMVLKRLLP"
BASE COUNT         2625 a         3069 c         2739 g         2436 t
ORIGIN      
        1 ccgttgtagt cagaattttc aattaccgta aagagatatt cgtaattaat ttctccggtt
       61 cccggttcgc cgcgatgcgg attatcagcg atttgcaggt gaccaatttt atcggcccat
      121 tgcgtcatag tgttggttaa ttcaccctcc atccgctgca tgtgataaat gtcatactga
      181 attttcaaat tgcagcaacc aacatcatca atcaatttca gcgcctgccg agttccggtg
      241 agatggaaac caggaatatc aaaatggttg ataggttcaa tcagtaataa aatatcttct
      301 ttcatcagca tattcgcggc ataacgcagg ttttctacaa gcgttgcgtg aatctgttca
      361 ctgctgaaac cagccggcgt tttaccgacc agacagttaa tttttttatt acccagcgca
      421 cgagcataac gaatcgctgc tgctacgcca tcccgaaact cttcttcacg gccaggaata
      481 caggcaatac cccgctcgcc cgccgcccag tcacctgccg gtaaattgtg cagcgtatgt
      541 tcgagtttat tactcgccag cacctgtttt aattcttcaa tgtcgtagtc ataagggaac
      601 ataaattcaa cgccgcgaaa accacactgc gcagctttct caaaacgggc gagaaaatca
      661 tattcaccaa ataacatcga taaattagca gagaaacgta acattattta tctcccttat
      721 tcatagtgca tgaaacaggt ttcagtcggt gcgtccgctg cgttatcggc gatatcttca
      781 aattccatga cgttatccag ttcactgccc atcgaaatat tggtcacacg ctcgagaata
      841 acttccacga ctacaggtac ccgatattgc gctattaaga ctttcgcctg ttcaaaggct
      901 ggtgcaatat cttccggttt gaaaacgcga attgctttac aacctaaacc ttccgctact
      961 tttacgtggt cgacgccata accgttcact tcgctggagt taatattttc gaaagcgagc
     1021 tgtacgcagt aatccatatc aaacgcgcgc tgcgactggc gaatcagccc cagataagcg
     1081 ttgttcacca gtacatggat gtacggaata ttgaactgtg cgccaacggc taattcttca
     1141 atcaggaact ggaagtcgaa gtcaccggaa atcgccacca cattgcgctc tggatcagcg
     1201 gcacacacgc ccagcgccgc cggaatggtc cagcccaacg ggcctgcctg cccacagttg
     1261 atccagtggc ggtctttaaa gacatgcagc atttgtgccg cagcgatttg tgacagacca
     1321 atggtggtga cataacaaac atcgcgacca aaggctttgt tcatctcttc atacacgcgc
     1381 tgcggtttca ccggcacatt gtcgaagtgg gttttgcgca gcaaagtgcg tttgcgctgc
     1441 tggcagtcgg cgacccattc tttacgacac ggcagacgac ccgctttttg catctcctgc
     1501 gccacttcaa ccagcagcgt cagcgctgct ttagcatcag agacaatgcc gagatccgga
     1561 cacagcacgc ggccaatttg cgtcggctca atatcgatat gaacgatttt gcgcccttcg
     1621 gtgtatttct ctaccgaacc ggtatgacgg ttagcaaaac ggttaccgat accaaacacc
     1681 atgtcggacg ccagcagcgt tgcgttaccg taacgatgcg cggtttgcag acccaccatc
     1741 ccggccatca gttcatgatc gtcgggaata cagccccagc ccatcagcgt tgggatcacc
     1801 ggaacgctgg tcagttcagc aaactgttgt aacagtgcag cagcgtcagc attgattacc
     1861 ccgcccccgg caacaatcac cggacgttcg gcctgaatta acatttctac agctttttcg
     1921 atctgcatac ggctggcagc aggtttgtag accggcagcg gttcgtacat gtcaggatca
     1981 aactcgattt ccgcaacctg aacgtcgaac ggtaaatcca ccagtaccgg acccgggcga
     2041 ccagaacgca tcaggtgaaa cgcctgttgc agcacacgag gcaccagcgc cgcttcacga
     2101 actgtaaccg ccattttgct gaccggttta gcaattgctt caatatcaac ggcctggaaa
     2161 tcttctttat gcagacgggc gcgcggaacc tggccggtaa tacacagaat aggaatggaa
     2221 tcagcagaag cagaatagag cgcggtgatc atgtccgtgc ccgcaggacc ggaagtcccc
     2281 agacatacgc cgatattccc tgccgttgcg cgggtataac cttccgccat gtgcgaagca
     2341 ccttccacat gacgcgccag aatgtgacga ataccgccgt gcttacgcat cgctgagtag
     2401 aacggattga ttgcagctcc cggaacgccg aaggcggtga tgataccttc tttctccagc
     2461 acatacattg ccgcgtcaac ggctctcatt tttgccattt tattcctacc tctattaatt
     2521 ggaaaaattt tccaactttc aaattttgtc aaaaaaaccc gctccgcaga gcgggatgcg
     2581 acagacacta tggatgtgct ttcagtccca acgccgtgct gatatcgcgg gcggtgtctc
     2641 tgaccagctc accctgactg acaaaacgat cttctgtcag tcttgatgaa ggcccggaga
     2701 tggagatagc ggcaacaaca ctacctacat catcgtaaat tgctgaagct atgcaattca
     2761 ggccaacaac atgctcttct ttatctacgg tatagcccag ttcacgcgct tgttccaggt
     2821 ccttcagcaa ggtgggcata tccacaagcg tcgttggcgt aaactgctgc aaaccggttt
     2881 gcagaatgat gctcatcagc tcctcttccg ccagcggata aagcagcgcc ttgcccgcac
     2941 cggaagcatg cagcggcaga cgactgccca gcggcgcaca catcctgacc atcgatttac
     3001 actctaactg accaattaat accgcttcat tgccgttacg gatcgcgaca ttgaccgttt
     3061 cgccggaaag taacatcagg cggcgcataa acggcccggc gacggagagg acatcgcggt
     3121 tatggatgta cgctgcaccg acgttaaaga cacctaatcc tatatgccac cagcctaact
     3181 gactgtcctg atagacaaaa tccgctgcct gtaaaacctt cagcaagcga aaggtcgtgg
     3241 agagcggcaa atccagattg agagaaatat cgctaaccga tgaacttccc ccactttttt
     3301 ccagatattg cagaatcgca attccccgct ctaacgcctg tgcgcccttc tgtgccacag
     3361 gctccgcctg tcctggcctg ccgcgccgtc taacttccgt catacctacg ttaactccga
     3421 cctacaagtg agcataaaca atgaacaatt cgtttatctt atgcaagtcg gttgcaggcg
     3481 tcacgcaaaa cagagttcct gttcaggaat actttcaaca tcacagttgt cactgccgcc
     3541 gcgatcgatg gtcaagaaat cggtgacgcg ctgccaggcg aaaagtggat gatgccagac
     3601 gttacgatgg taattcaccc cctgttcgcc gttggtaata aacgcccgca gagttgacag
     3661 gtctggtttg tcgtcaccta acgccacgac caccacaaac acttcccctt tcatcgggat
     3721 aaaggcctga gtacccagcg gatgacgttc gagttcgtga agggtcagcg gcagattcgc
     3781 cggttgcgcg cggttaatgc tgataagcgt acggtcttgc tcaagaatct caaccagcgc
     3841 caaatcgtgg taacgctcca ccagtccatt gttaatatgg aaaaaatccc gttgctgcgt
     3901 ttcgattacg tcgccataag cactaaaggc ttcctgactt aacggtaata cctgaagttt
     3961 catctttttg tccttgtaac ataattccgt tcaaccaccg ccgaccggtc agcttggttt
     4021 caataacagg caatctaaaa ccttaagaga acaacatcaa cacaaaagtt ggaatatttt
     4081 tccaaaacta gatttagtcc acaaattgaa cagggttatc atgtaaaaat actgataaat
     4141 cagaggataa gaaaatcacg caacttgcgg atttaaaagg ggatacctga agatgttcga
     4201 tccagaaacc ttgcggactt tcattgcggt tgctgaaaca ggtagttttt caaaagcggc
     4261 agaacgatta tgtaaaacca cggcgacgat cagttatcgc attaaacttc tggaagagaa
     4321 taccggagta gcgctgtttt tccgtacgac tcgtagcgta acgttgacag cggctggcga
     4381 gcatctactt tcccaggcca gagactggct gagctggctg gaaagtatgc caagcgagct
     4441 gcaacaggtg aatgatggcg tggaacgcca ggtgaatatt gtcatcaaca acctgctcta
     4501 taacccccag gccgttgccc agttgctggc gtggctgaat gagcgttacc cctttaccca
     4561 gtttcacatc tcccgacaaa tctatatggg cgtctgggac tcgctattgt acgaaggttt
     4621 ttcgctggct atcggcgtca cgggaactga ggcgctggca aataccttta gtcttgatcc
     4681 cttaggatcg gtgcaatggc gctttgtcat ggcggcggat catccgctgg cgaacgttga
     4741 agagccgcta acagaagcgc agttgcggcg ctttccggcg gtcaatattg aagacagcgc
     4801 ccgcacacta accaaacgcg tcgcctggcg attgccgggg caaaaagaga ttattgttcc
     4861 cgatatggaa acgaaaatcg ccgcccatct ggcgggcgtt ggcattggtt ttttgccaaa
     4921 atcactttgc cagtcaatga tcgataatca acaactggtc agccgggtaa tcccaacgat
     4981 gcgccctcct tcaccattga gtctggcatg gcgcaaattt ggcagcggca aagcgataga
     5041 agatattgtg accttgttta cccagcgcag gccggaaatc agcggatttt tagaaatttt
     5101 cggcaaccca cgcagttaaa aatcaagttt ccttgcgcgt tggcgcgtaa aatagcgcgc
     5161 aagctgattt gaggttcact ttttactatg caagagagac acacggaaca ggactatcgt
     5221 gccctgctga ttgctgatac gcccattatt gatgttcgcg cccccatcga gtttgagcaa
     5281 ggcgcaatgc ctgccgctat caacctgccg ttaatgaata acgatgaacg cgccgccgtt
     5341 ggcacctgct ataaacagca agggtcagac gcggcgctgg cgctgggtca taaactggtg
     5401 gcgggtgaaa ttcgtcagca gcgcatggac gcctggcggg cagcgtgcct gcaaaatccg
     5461 caaggtattc tctgctgcgc ccgaggcggt cagcgctcac atattgtcca aagctggttg
     5521 catgcagcgg ggattgatta tccgctggtg gaaggcggtt ataaggcact gcgccagacc
     5581 gcgattcagg cgactattga actggcacaa aaaccgatag tgctgattgg cggttgtacc
     5641 ggcagcggta aaacgctgtt agtgcagcaa cagccgaacg gtgttgatct ggaagggttg
     5701 gcgcgtcatc gcggttcggc gtttggtcgc accttacaac cacaacttag ccaggcgagt
     5761 tttgaaaacc tgctggctgc cgaaatgcta aaaaccgacg cccgtcagaa tttgcgcctg
     5821 tgggtgctgg aagacgaaag ccggatgatc ggttcgaatc acctgccgga atgcctgcgc
     5881 gagcgaatga ctcaggcggc gattgcggtg gtagaagatc cgtttgagat ccgtcttgag
     5941 cgcctgaacg aagagtattt cttgcgtatg catcatgatt ttacccacgc gtacggcgac
     6001 gaacagggct ggcaggagta ttgcgaatac ctgcatcacg gactttcggc tattaagcgt
     6061 cggctggggc tacagcgata taacgaactg gctgcaaggc tggatgcagc actgacaacg
     6121 caactcacca ccggcagcac cgacggtcat ctggcctggc tggtgccgtt acttgaagaa
     6181 tattacgacc cgatgtatcg ctatcagctt gagaaaaaag cggaaaaagt tgtctttcgc
     6241 ggtgagtggg cggaagtggc ggaatgggtg aagtcgcggt aataggcggt attctgtgca
     6301 cgctgttcat gccggatgcg gcgtgaacgc cttatccggc ctacggttaa gtgcagtttt
     6361 gtaggcctga taagacgcgc cagcgtcgca tcaggcaatc taacgcctgt catcatcccg
     6421 caaactgcct gaacagcgcc ttacccttaa ccagtcgcgc acccagccag ccgccgcaaa
     6481 gcgacagcag cagcgcaccg ctgcacggca gcacaatcca caatcgccag tctggctccc
     6541 acgggaaatc aaacactttc gcctgcaaca ctgccagcgc cgtttccgca ccaattgcgg
     6601 ccaccaggcc agaaacaaac ccaagcatgg cgaactcaca ccacaatgtc gtgcgcagca
     6661 gctttttccc cgcaccgagt gtgcgccaca ccaccagctc ctgatgacgc tgacgcattc
     6721 ccacctgcac ctgtgccagc aacagcaaca taccgcaggc ggtgaccagc accaccatca
     6781 cctccagcgc ccgacttacc tgctccagca cctgaccgac ctgttttaaa atcgcgccaa
     6841 tgtctaacag gctaatggtc gggaactggc ggttgagttg cgtcaacatg ccgttgccat
     6901 tctcccagcg gaaactggta agccagctct gcggttgccc gtctaatgcc ccttcaggga
     6961 aaataaaata gaaattaggc cgcagacttt cccagtccac tttgcgcagg ctggtcactt
     7021 tagcgcggaa ctcctgggta tcgcccataa aagtcacggt atcaccgagg gcaacgttta
     7081 agcgtttcgc cagcccctct tccatcgaca cttcatcggc ttttggcggc cagttaccgg
     7141 cgacaatcgg attatgatcg ggccgcgtat tttgccaggt aagattgagt tcgcgattga
     7201 gcgcctcatc ttcattgcct tctgtcggct tgccgttaat cgccgtcagt ctcgcccgca
     7261 ccaccggata aaacgattcc gggactatct gatgttccgc gaggaacgct tttagcggtg
     7321 ctacctgttc tgtggcgatg ttaattaaaa agtagttcgg gctttctgga ggtagctgct
     7381 gctgccagcg gtcgagcaga tcgccacgca acaccagcag cagtgccagc agcataaagg
     7441 agagcgaaaa tgccgaaagc tggcttaacg ttgaccacgg ctgacgtaac aggcggctaa
     7501 ccgccaggcg cagaggcagc gatttcagcg tcatgcggcg aagtacattc agcagcatcc
     7561 agcccagcac accgcacagc aaagccagta ctaccgcgcc cgccagcacc gcccaaagca
     7621 gcatgctgcc acccattaat ccggcgagca gcagcacaac cactacactg acaatcggca
     7681 gataaaactt cagcggccag acgttcgcta ccacatcatt acgtaatacg cgtaaaggct
     7741 gcgttgccag caacaagcgg tatggtcgta gccccaccag cagcgagata accgtcatgg
     7801 tgccgagcgc ccacagccac ggccagaggc tggctggcgg tagtgcggca ggcagaacgg
     7861 gcttgagcag caccatcaac acgttttcga acaacaggcc tatcgcccca ccggtaacgg
     7921 ctgaaagcgt cagcaccatc aaccactgac cgacgattag cttacgcagt tgcgctcgcc
     7981 ctgcccccag cgttttgagg atcgccacca gatcgtagcg actgcgacag taatgattca
     8041 tcgccaccgc caccgctgcc actgccagca gcaaggtcag aagcgcagaa agcagcagga
     8101 actgttgcga gcgttccatc gagcgcccca gcgcgccttc gtcctgttcc agaccgtacc
     8161 agcgttgttc gggtttaagc tggggtaaca accatttctc atagccgtcg agctggttct
     8221 cgttgccgcc gaatttatag cgccaggtga cccgacttcc cggctgcacg gctccggttt
     8281 tatcgacatc cgccagattc atcatcagac gcggagccat ctggaagggg ttaaaaccgg
     8341 aatccggttc ctgaatcact tctccggcaa tccgcaaggt ggcatcgccc acgtcaatgg
     8401 tgtcgcccgt tttcaggtta agcagtgcca tcaggcgtgg ggccagcaat acgctgcccg
     8461 cctgcggttt caggcctggg ggattggttt gcagatcgcc atacatcggg tagatatcat
     8521 ccaccgcttt gacgttcgcc agctgcggtg tgtcgcctgc aaatgtcatg gtggcgaaag
     8581 tcagctgctt gccgactttc aggccgcgct tttgcgcttc ctccagccac gcttgcggca
     8641 cttcgcgcga actgcgcaac gcccgatcgc ccgccataaa ctcccggctt tgctggctta
     8701 agcccttctc catgcgatcg ctgatattgc ccagcgccag cacgcaggcc accgccaggc
     8761 ttagcgccag ccagacaatt aatagcgacg gcgagcgcca ttcgcgccag aaccaacgtg
     8821 caatcatgtt tcctcctgca actgcccgtt caccagccgt aagcagcggt cgcagcgtgc
     8881 cgccagttgc aggtcgtggg tcaccataat caacgtggtg ccatgttcac ggttgaggga
     8941 aaacagcagg tcggcaattt tatcgcccgt ctggcggtca agattgccgg tgggttcgtc
     9001 ggcaaacagc acatcaggcc gaccattaaa agctcgcgcc agcgccactc gttgctgttc
     9061 gccgccggaa agctgtgccg gaagatgatc cagacgttta cccagtccta actgttcgag
     9121 caacgctttt gccccgttac gactttctgc gctactctca ccacgcagca gcgccggaag
     9181 ctcgacgttt tccagcgcgt taagggtagg aattaacata aatgactgaa aaacaaagcc
     9241 tacgtgcttc gcgcgcaact ttgcccgcgc ttcttcgtcc atattatgta gcggttgtcc
     9301 caccagactc acttcgccac tgctgccgtc atccagcccg gcgaggatcg ccagcaaggt
     9361 tgacttaccc gatcccgact cgcccaccag cgcgatggtc tcgccacgtt tgacaaccag
     9421 ctcaactccg gtgaggatgg agagttcatg ctccccctga ccgacggact tcttaagatg
     9481 atgaacttca acaatgtttt ccgctggcat ttgcccttcc tgttcctggt cctgttaacc
     9541 ttccgtgccg ctgcagcgga cacgttattg attctgggtg atagcctgag cgccgggtat
     9601 cgaatgtctg ccagcgcggc ctggcctgcc ttgttgaatg ataagtggca gagtaaaacg
     9661 tcggtagtca atgccagcat cagcggcgac acctcgcaac aagggctggc gcgccttccg
     9721 gctctgctga aacagcatca gccgcgctgg gtgctggtag aactgggcgg caatgacggt
     9781 ttgcgtggtt ttcagccaca acaaaccgag caaacgctgc gccagatttt gcaggatgtc
     9841 aaagccgcca acgctgaacc attgttaatg caaatacgtc tgcctgcaaa ctatggtcgc
     9901 cgttataatg aagcctttag cgccatttac cccaaactcg ccaaagagtt tgatgttccg
     9961 ctgctgccct tttttatgga agaggtctac ctcaaaccac aatggatgca ggatgacggt
    10021 attcatccca accgcgacgc ccagccgttt attgccgact ggatggcgaa gcagttgcag
    10081 cctttagtaa atcatgactc ataaagcaac ggagatcctg acaggtaaag ttatgcaaaa
    10141 atcggtctta attaccggat gttccagtgg aattggcctg gaaagcgcgc tcgaattaaa
    10201 acgccagggt tttcatgtgc tggcaggttg ccggaaagcg gatgatgttg agcgcatgaa
    10261 cagcatggga tttaccggcg tgttgatcga tctggattca ccagaaagtg ttgatcgcgc
    10321 agccgacgaa gtgatcgccc tgaccgataa ttgtctgtat gggatcttta acaatgccgg
    10381 attcggcatg tatggccccc tttccaccat cagccgtgtg cagatggaac agcagttttc
    10441 cgccaacttt ttcggcgcac accagctcac catgcgcctg ttacccgtga tgttaccgca
    10501 cggtgaagga cgtattgtga tgacctcatc ggtgatggga ttaatctcca cgccgggtcg
    10561 tggcgcttac gcggccagta aatatgcgct ggaggcgtgg tcagacgcac tgcgcatgga
    10621 gctacgtcac agcggaatta aagttagcct gatcgaaccc ggtcccattc gtactcgctt
    10681 caccgacaac gtcaaccaga cgcaaagtga taaacctgtc gaaaatcccg gcatcgccgc
    10741 ccgctttacg ttgggaccgg aagcggtggt cgacaaagtg cgccatgctt ttattagcga
    10801 gaagccgaag atgcgctatc cagtaacgct ggtgacctgg gcagtaatgg tgcttaagcg
    10861 cctgctgcc
//
LOCUS       DAMZFT010000157        10795 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.157, whole genome shotgun sequence.
ACCESSION   DAMZFT010000157 DAMZFT010000000
VERSION     DAMZFT010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10795)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10795)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10795
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.157"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..1209
                     /locus_tag="PN977_004308"
     CDS             <1..1209
                     /locus_tag="PN977_004308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-dependent hydrogenase large subunit"
                     /protein_id="HDI6044269.1"
                     /translation="PEANLMGFAHYLEALDFQREIVKIHAVFGGKNPHPNWIVGGMPC
                     AINIDESGAVGAVNMERLNLVQSIITRTADFINNVMIPDALAIGQFNKPWSEIGTGLS
                     DKCVLSYGAFPDIANDFGEKSLLMPGGAVINGDFNNVLPVDLVDPQQVQEFVDHAWYR
                     YPNDQVGRHPFDGITDPWYNPGDVKGSDTNIQQLNEQERYSWIKAPRWRGNAMEVGPL
                     ARTLIAYHKGDAATVESVDRMMSALNLPLSGIQSTLGRILCRAHEAQWAAGKLQYFFD
                     KLMTNLKNGNLATASTEKWEPATWPTECRGVGFTEAPRGALGHWAAIRDGKIDLYQCV
                     VPTTWNASPRDPKGQIGAYEAALMNTKMAIPEQPLEILRTLHSFDPCLACSTHVLGDD
                     GSELISVQVR"
     gene            1228..1935
                     /gene="hyaC"
                     /locus_tag="PN977_004309"
     CDS             1228..1935
                     /gene="hyaC"
                     /locus_tag="PN977_004309"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase b-type cytochrome subunit"
                     /protein_id="HDI6044270.1"
                     /translation="MQQKSDNVVSHYVFEAPVRIWHWLTVLCMAVLMVTGYFIGKPLP
                     SVSGEATYLFYMGYIRLIHFSAGMIFTVVLLMRIYWAFVGNRYSRELFIVPVWRKSWW
                     QGVWYEIRWYLFLAKRPSADIGHNPIAQAAMFGYFLMSVFMIITGFALYSEHSQYAIF
                     APFRYVVEFFYWTGGNSMDIHSWHRLGMWLIGAFVIGHVYMALREDIMSDDTVISTMV
                     NGYRSHKFGKISNKERS"
     gene            1932..2519
                     /locus_tag="PN977_004310"
     CDS             1932..2519
                     /locus_tag="PN977_004310"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015872616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 maturation protease"
                     /protein_id="HDI6044271.1"
                     /translation="MSEQRVVVMGLGNLLWADEGFGVRVAERLYAHYHWPEYVEIVDG
                     GIQGLNLLGYVESASHLLILDAIDYGLEPGTLRTYAGERIPAYLSAKKMSLHQNSFSE
                     VLALADIRGHLPAHIALVGLQPAMLDDYGGSLSELAREQLPAAEQAALAQLAAWGIVP
                     QPANESRCLNYDCLSMENYEGVRLRQYRMTLEEQE"
     gene            2516..2914
                     /gene="hyaE"
                     /locus_tag="PN977_004311"
     CDS             2516..2914
                     /gene="hyaE"
                     /locus_tag="PN977_004311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-1 operon protein HyaE"
                     /protein_id="HDI6044272.1"
                     /translation="MSNDTPFDALWQRMLARGWTPISESRLDDWLTQAPDGVVLLSSD
                     PKRTPEVSDNPVMIGELLREFPDYTWQVAIADLEQSEAIGDRFGVFRFPATLVFTGGN
                     YRGVLNGIHPWAELINLMRGLVEPQQERAS"
     gene            2911..3768
                     /locus_tag="PN977_004312"
     CDS             2911..3768
                     /locus_tag="PN977_004312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein"
                     /protein_id="HDI6044273.1"
                     /translation="MSETFFHLLGPGTQPNDDSFSMNPLPITCQVNDEPSMAALEQCA
                     HSPQVIALLNELQHQLSERQPPLGEVLAVDLLNLNADDRHFINTLLGEGEVSVRIQQA
                     DDSESEIQEAIFCGLWRVRRRRGDKLLEDKLEAGCAPLALWQAATQNVLPTDSLLPPP
                     IDGLMNGLPLAHELLAHVRNPDAQPHSINLTQLPISEADRLFLSRLCGPGNIQIRTIG
                     YGESYINATGLRHVWHLRCTDTLKGPLLESYEICPIPEVVLAAPEDLVDSAQRLSEVC
                     QWLAEGAPT"
     gene            3902..5446
                     /gene="appC"
                     /locus_tag="PN977_004313"
     CDS             3902..5446
                     /gene="appC"
                     /locus_tag="PN977_004313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit 1"
                     /protein_id="HDI6044274.1"
                     /translation="MWDVIDLSRWQFALTALYHFLFVPLTLGLIFLLAIMETIYVVTG
                     KTIYRDMTRFWGKLFGINFALGVATGLTMEFQFGTNWSFYSNYVGDIFGAPLAMEALM
                     AFFLESTFVGLFFFGWQRLNKYQHLLVTWLVAFGSNLSALWILNANGWMQYPTGAHFD
                     IDTLRMEMTSFSELVFNPVSQVKFVHTVMAGYVTGAMFIMAISAWYLLRGRERDVALR
                     SFAIGSVFGTLAIIGTLQLGDSSAYEVAQVQPVKLAAMEGEWQTEPAPAPFHVVAWPE
                     QDQERNAFAIKIPALLGILATHSLDKPVPGLKNLMAETYPRLQRGRMAWLLMQEISQG
                     NREPHVLQAFRELEGDLGYGMLLSRYAPDMNHVTATQYQAAMRGAIPQVAPVFWSFRI
                     MVGCGSLLLLVMLIALVQTLRGKIDQHRWVLKMALWSLPLPWIAIEAGWFMTEFGRQP
                     WAIQDILPTYSAHSALTTGQLAFSLIMIVGLYTLFLIAEVYLMQKYARLGPSAMQSEQ
                     PTQQQG"
     gene            5458..6594
                     /gene="appB"
                     /locus_tag="PN977_004314"
     CDS             5458..6594
                     /gene="appB"
                     /locus_tag="PN977_004314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HDI6044275.1"
                     /translation="MFDYETLRFIWWLLIGVILVVFMISDGFDMGIGCLLPLVARNDD
                     ERRIVINSVGAHWEGNQVWLILAGGALFAAWPRVYAAAFSGFYVAMILVLCSLFFRPL
                     AFDYRGKIADARWRKMWDAGLVIGSLVPPVVFGIAFGNLLLGVPFAFTPQLRVEYLGS
                     FWQLLTPFPLLCGLLSLGMVILQGGVWLQLKTVGVIHLRSQLATKRAALLVMLCFLLA
                     GYWLWVGIDGFVLLAQDANGPSNPLMKLVAVLPGAWMNNFVESPVLWIFPLLGFFCPL
                     LTVMAIYRGRPGWGFLMASLMQFGVIFTAGITLFPFVMPSSVSPISSLTLWDSTSSQL
                     TLSIMLVIVLIFLPIVLLYTLWSYYKMWGRITTETLRRNKNELY"
     gene            6607..6699
                     /gene="cbdX"
                     /locus_tag="PN977_004315"
     CDS             6607..6699
                     /gene="cbdX"
                     /locus_tag="PN977_004315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit CbdX"
                     /protein_id="HDI6044276.1"
                     /translation="MWYLLWFVGILLMCSLSTLVLVWLDPRLKS"
     gene            6779..8089
                     /gene="appA"
                     /locus_tag="PN977_004316"
     CDS             6779..8089
                     /gene="appA"
                     /locus_tag="PN977_004316"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001623747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acid phosphatase/4-phytase"
                     /protein_id="HDI6044277.1"
                     /translation="MKAILIPFLSLLIPLTPQSAFAKSEPELKLESVVIVSRHGVRAP
                     TKATQLMQDVTPDAWPTWPVKLGWLTPRGGELIAYLGHYQRQRLVADGLLAKKGCPQP
                     GQVAIIADVDERTRKTGEAFAAGLAPDCAITVHTQADTSSPDPLFNPLKTGVCQLDNA
                     NVTDAILSRAGGSIADFTGHRQTAFRELEQVLNFPQSNLCLNREKQDKSCSLTQALPS
                     ELKVSADNVSLTGAVSLASMLTEIFLLQQAQGMPEPGWGRITDSHQWNTLLSLHNAQF
                     YLLQRTPEVARSRATPLLDLIKTALTPHPPQKQAYGVTLPTSVLFIAGHDTNLANLGG
                     ALELNWTLPAQPDNTPPGGELVFERWRRLSDNSQWIQVSLVFQTLQQMRDKTPLSLNT
                     PPGEVKLTLAGCEERNAQGMCSLASFTQIVNEARIPACALHQDK"
     gene            complement(8077..8289)
                     /gene="cspH"
                     /locus_tag="PN977_004317"
     CDS             complement(8077..8289)
                     /gene="cspH"
                     /locus_tag="PN977_004317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspH"
                     /protein_id="HDI6044278.1"
                     /translation="MSRKMTGIVKTFDRKSGKGFIIPSDGRKEVQVHISAFTPRDAEV
                     LIPGLRVEFCRVNGLRGPTAANVYLS"
     gene            8575..8787
                     /gene="cspG"
                     /locus_tag="PN977_004318"
     CDS             8575..8787
                     /gene="cspG"
                     /locus_tag="PN977_004318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock protein CspG"
                     /protein_id="HDI6044279.1"
                     /translation="MSNKMTGLVKWFNADKGFGFITPDDGSKDVFVHFTAIQSNEFRT
                     LNENQKVEFSIEQGQRGPAAANVVTL"
     gene            8798..8986
                     /locus_tag="PN977_004319"
     CDS             8798..8986
                     /locus_tag="PN977_004319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HDI6044280.1"
                     /translation="MTQHLHFRCPCCHGSQYRTSAFDVTERNPLGAKCIFCKSTMITF
                     DNVALQIRTEHAPLDFTK"
     gene            8961..9191
                     /gene="ymcE"
                     /locus_tag="PN977_004320"
     CDS             8961..9191
                     /gene="ymcE"
                     /locus_tag="PN977_004320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmcE"
                     /protein_id="HDI6044281.1"
                     /translation="MRRWISQNNIRLPRGAFFISALFFFNAVCIVSDNLLIIESFGEM
                     AYNISYLTRVPGTNTLLACCCLSRPDEVNSEY"
     gene            9181..9354
                     /locus_tag="PN977_004321"
     CDS             9181..9354
                     /locus_tag="PN977_004321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module toxin, GnsA/GnsB family"
                     /protein_id="HDI6044282.1"
                     /translation="MNIEELKKQAETEIADFIAQKIAELNKNTGKEVSEIRFTAREKM
                     TGLESYDVKIKIM"
     gene            complement(9403..10476)
                     /locus_tag="PN977_004322"
     CDS             complement(9403..10476)
                     /locus_tag="PN977_004322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HDI6044283.1"
                     /translation="MAEKKRTRWQRRPGTTGGKLPWNDWRNATTWRKATQLLLLAMNI
                     YIAITFWYWVRYYETAGSTTFVARPGGIEGWLPIAGLMNLKYSLATGQLPSVHAAAML
                     LLVAFIVISLLLKKAFCSWLCPVGTLSELIGDLGNKLFGRQCVLPRCLDIPLRGVKYL
                     LLSFFLYIALLMPAQAIHYFMLSPYSVVMDVKMLDFFRHMGTATLISVTVLLIASLFI
                     RHAWCRYLCPYGALMGVVSLLSPFKIRRNAESCIDCGKCAKNCPSRIPVDKLIQVRTV
                     ECTGCMTCVESCPVASTLTFSLQKPAANKKAFALSGWLMTLLVLGIMFAVIGYAMYAG
                     VWQSPVPQELYRRIIPQAPMIGH"
     gene            complement(10559..>10795)
                     /locus_tag="PN977_004323"
     CDS             complement(10559..>10795)
                     /locus_tag="PN977_004323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001054736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HDI6044284.1"
                     /translation="KQHALPLLDDIDIARASQNSEKIKRAAHQLKSSCSSLGMRSASQ
                     LCAQLEQQPLSAPLPHEEITRSVAALEAWLIRKT"
BASE COUNT         2507 a         2690 c         2939 g         2659 t
ORIGIN      
        1 ccagaagcca acctgatggg ctttgcccac tatctcgaag ctctcgattt ccagcgtgaa
       61 attgtcaaaa tccacgcggt ctttggcggt aaaaacccac atccaaactg gattgtcggc
      121 gggatgcctt gtgccattaa cattgacgaa agcggcgcgg tcggggcggt caatatggaa
      181 cgcctgaacc tggtgcagtc aatcattacc cgtacggcgg atttcattaa caacgtgatg
      241 atccccgacg ccttagccat cggtcagttc aacaagccgt ggagcgaaat cggcactggc
      301 ctttcggata aatgcgttct cagttacggc gcgttcccgg atatcgccaa cgactttggc
      361 gagaaaagtc tgctgatgcc tggcggcgcg gtgattaacg gcgacttcaa caatgtgctg
      421 ccagtggatt tggttgatcc gcagcaggtg caggagtttg tcgatcacgc ctggtatcgt
      481 tatcccaacg atcaggtcgg gcgtcatccg ttcgatggta ttaccgaccc gtggtacaac
      541 cccggcgatg tcaaaggcag cgataccaac attcagcagc tgaatgaaca ggaacgctac
      601 tcgtggatca aagcgccacg ctggcgcggt aacgcgatgg aagtggggcc gctggcacgc
      661 acgttaatcg cttatcacaa aggcgatgcg gcgaccgttg agtcggtcga tcgcatgatg
      721 tcggcgttga acctaccgct ttccggcatc cagtcaacgt taggccgcat tttgtgtcgc
      781 gcgcacgaag cgcagtgggc cgcaggcaag ttgcaatatt tcttcgacaa gctgatgacc
      841 aatctcaaaa acggcaatct cgccaccgcc tccactgaga agtgggaacc agcaacctgg
      901 ccgacagagt gccgtggtgt cggctttacc gaagcgccgc gcggagcgtt aggccactgg
      961 gccgccattc gcgatggcaa gattgatctc taccagtgcg tggtgccgac cacctggaac
     1021 gccagcccgc gcgatcctaa agggcaaatt ggcgcttatg aagcggcgct gatgaacacc
     1081 aaaatggcga tccccgagca accgctggag atcctgcgta ctctgcacag ctttgatccg
     1141 tgcctcgcct gttcaacaca cgtgctgggc gacgacggta gcgagctgat ctccgtgcag
     1201 gtgcgttaac agcgaaggag aatcatcatg caacagaaaa gcgacaacgt tgtcagccac
     1261 tatgtctttg aagcgccagt gcgtatctgg cactggctga cggtgttatg catggcggtg
     1321 ttgatggtca ccggttactt catcggcaaa ccgctgcctt cggtcagcgg tgaggcgacg
     1381 tatctgtttt acatgggcta tatcaggttg attcacttca gcgccgggat gatttttacc
     1441 gtggtcttgc tgatgcggat ctactgggct tttgtcggca atcgctactc acgcgagctt
     1501 ttcatcgtgc cagtatggcg taaaagctgg tggcagggcg tgtggtatga aatccgctgg
     1561 tatctgtttc tggcaaaacg tccaagtgcc gatataggcc ataaccccat cgcccaggcg
     1621 gcgatgttcg gctatttcct gatgtcagtt tttatgatca tcaccggctt tgcgctgtac
     1681 agcgaacaca gccagtacgc tatttttgcg ccgttccgtt atgtggtgga atttttctac
     1741 tggacgggtg gcaactcaat ggacattcac agctggcatc ggctggggat gtggctgatt
     1801 ggcgcgtttg tgatcggtca tgtctacatg gcgctgcgtg aagacatcat gtccgacgac
     1861 acggtgatct ccactatggt caacggctac cgtagccaca aatttggcaa aataagtaac
     1921 aaggagcgtt catgagcgag caacgcgtgg tggtaatggg gctgggcaac ctgctgtggg
     1981 ccgatgaagg tttcggcgtg cgggtggcgg aacggttgta tgcccattac cactggcccg
     2041 agtatgtgga gattgtcgat ggcggtattc agggactgaa cttgctgggg tatgtcgaaa
     2101 gcgccagcca tctgttgatt ctcgatgcca ttgactacgg gctggaacct ggaacgctgc
     2161 gaacctatgc cggagaacgt attccggctt atctcagcgc gaagaaaatg agcctgcacc
     2221 agaacagttt ctccgaagtg ctggcgctgg cagatatccg cggacatctg ccagcacata
     2281 ttgccctcgt cggtctgcaa cccgcaatgc tcgacgacta cggcggcagc ctgagcgaac
     2341 tggcacggga gcaactgccc gcagcggaac aggcggcgct ggcgcagttg gctgcgtggg
     2401 gaattgtgcc gcaaccggct aatgaatcgc gctgtctcaa ttatgactgt ctgtcgatgg
     2461 aaaattacga aggcgttcgc ttgcgccagt acaggatgac gctggaggag caggaatgag
     2521 caacgacacg ccatttgatg cgttgtggca acgtatgctg gcgcgcggct ggacgccgat
     2581 cagtgaatcc cgtcttgacg actggcttac gcaagcgcca gacggcgtgg tgttattaag
     2641 tagtgacccg aaacgcacgc cagaagtcag cgataacccg gtcatgattg gcgaattact
     2701 gcgcgagttt cccgactata catggcaggt agcgattgct gatcttgagc agagcgaagc
     2761 catcggcgat cgctttggcg tctttcgctt tcccgccaca ttagtgttta ccggcggaaa
     2821 ctatcgcggc gtgctgaatg gtattcaccc gtgggcggaa ctgataaacc tgatgcgtgg
     2881 gcttgtcgaa ccacaacagg agcgtgcctc atgagcgaaa cttttttcca tctgctgggg
     2941 ccaggaacgc aaccgaacga tgacagtttc agcatgaatc cactgccgat cacctgtcag
     3001 gtgaatgatg aaccgagtat ggcggccctg gagcaatgtg ctcacagccc gcaggtgatt
     3061 gcgctgttaa acgagttaca acatcaacta agcgaacgcc agccgccgtt gggcgaggtg
     3121 ctggcagtcg atctgttaaa tctcaacgcc gacgatcgtc actttatcaa tacgcttctc
     3181 ggggaagggg aagtgtcagt gcgcatacag caggctgacg acagtgaaag tgaaatacag
     3241 gaggcgatct tctgcggatt atggcgggtg cgcagacgtc gcggcgacaa gttgctggag
     3301 gacaaactgg aggctggctg cgcgccgctg gcattgtggc aggcggcaac gcaaaacgtc
     3361 ttgccgacag attcgctgtt accgccgccc attgatggcc tgatgaatgg cctaccgttg
     3421 gcgcatgagt tactggcgca tgtacgtaac cccgacgcgc agccgcacag cattaatctg
     3481 acgcaattac ccatcagcga ggctgatcgg ctttttctct cacgtctctg tgggccggga
     3541 aatattcaga ttcgtaccat tggctatggc gagagctata tcaacgccac ggggttacgc
     3601 catgtctggc atttacgctg tacggacacc ttaaaaggcc cgttactgga aagttatgaa
     3661 atctgcccaa taccggaagt ggtgctggca gcgccagaag atttggtcga ctctgcgcag
     3721 cggcttagcg aggtatgtca gtggctggcg gaaggtgcac cgacataaaa agacggtaag
     3781 tatcgctttc agtcttatga atatcgcaat cggcgaatac ctctggtcgt agagtttcag
     3841 gataaagagg gagatctacc attatcgggt tatttttctc tcttcgccta caggagtgcg
     3901 catgtgggat gtcattgatt tatcgcgctg gcagtttgca ctgaccgcgc tgtatcactt
     3961 tttatttgta ccccttaccc tggggctgat ttttttgctg gctatcatgg aaaccattta
     4021 cgtggtcacc ggcaaaacaa tctaccgcga tatgacgcgc ttctggggta agctcttcgg
     4081 tatcaacttt gctcttggcg tggctaccgg cctgaccatg gagtttcagt ttggtactaa
     4141 ctggtcattc tattccaact atgtgggcga tatttttggc gcaccgctgg caatggaagc
     4201 attaatggcc ttcttcctcg aatccacctt tgtcgggctg ttcttcttcg gctggcagcg
     4261 gctgaataaa taccagcacc tgctggtgac gtggctggtg gcgttcggtt caaatctctc
     4321 tgcattgtgg atattgaatg ccaacggttg gatgcaatac ccgaccggtg cgcattttga
     4381 tatcgacacc ctgcgtatgg agatgaccag tttcagcgag ttggtcttta acccggtcag
     4441 ccaggtgaaa tttgtgcaca ccgtaatggc gggatacgtg accggggcca tgtttattat
     4501 ggcgatcagc gcctggtatt tactgcgcgg acgggagcgc gatgtcgcat tacgctcgtt
     4561 tgccatcggt tccgtcttcg gtactctggc gattatcggt acgctgcaac tcggagacag
     4621 ttctgcgtat gaagtcgcgc aagtacaacc ggtaaaactg gcggcgatgg aaggggagtg
     4681 gcaaacagaa cctgcacctg caccgttcca tgtggttgcc tggccggaac aggatcaaga
     4741 gcgtaacgcc tttgccatca aaattcccgc gctgctaggg atcctcgcca ctcactcatt
     4801 agataaaccc gtgccgggtc tgaagaattt gatggctgaa acctacccac gcttgcaacg
     4861 cggacgtatg gcctggctgt taatgcagga aatatcgcaa ggcaatcgtg agccgcatgt
     4921 gttgcaggca ttccgggagc tggaaggcga cctgggttac ggcatgttgc tctcccgcta
     4981 tgcgccggat atgaatcatg tcacagccac acagtaccag gcggcgatgc gtggcgcgat
     5041 acctcaggtt gcgccggtat tctggagttt ccgcatcatg gtaggctgtg gttccctgct
     5101 gttactggtg atgctgattg cgcttgtcca gacgctgcgt ggcaaaatcg accagcatcg
     5161 ctgggtgctg aaaatggcgc tctggagttt gccgcttccg tggattgcga ttgaagccgg
     5221 atggtttatg accgagtttg gtcgtcagcc gtgggcgata caggacatct taccgacata
     5281 ctccgcgcac tccgccttaa ccacaggaca actggctttc tcactgatca tgatcgtagg
     5341 gctttacacc ctgttcttaa tcgccgaagt ctacctgatg cagaaatatg cccgtctggg
     5401 gccgagcgcg atgcagagtg aacaaccgac gcagcaacag gggtaaagga gaaaatcatg
     5461 tttgattatg aaacattgcg cttcatctgg tggctgctga ttggcgtgat cctggtggtc
     5521 tttatgatct ccgacggatt tgacatgggg attggctgtt tgctgccgct ggtggcgcgt
     5581 aatgatgatg aacgccggat agtgataaac agcgttggcg cgcactggga aggcaatcag
     5641 gtctggttga tcctcgcagg tggggcatta tttgccgcct ggcccagagt gtatgcagcg
     5701 gcgttttccg gcttttatgt ggcgatgatt ctggtgctgt gctcgctgtt cttccgcccg
     5761 ctggcctttg attatcgcgg aaaaattgcc gatgcacgct ggcgtaaaat gtgggatgcc
     5821 ggtctggtca tcggcagtct ggtgccgccg gtagtcttcg gtatcgcctt cggcaacttg
     5881 ttgctcggcg tgccgtttgc ctttacaccg caattacgcg tggagtatct cggcagcttc
     5941 tggcaactgc tgacgccatt ccctttattg tgcggattgc tcagccttgg gatggtgatt
     6001 ttgcaaggtg gcgtctggtt acaactgaaa actgttggtg tgattcatct gcgttcacag
     6061 ctggcgacca aacgcgccgc actactggtg atgctgtgct ttttgctggc gggttactgg
     6121 ctgtgggtcg gtattgatgg ctttgtactg ctcgcccagg atgctaacgg tccttccaat
     6181 ccgttaatga aactggtggc agtgctacct ggtgcctgga tgaataattt cgtcgaatcg
     6241 cccgttttgt ggatcttccc gctgctgggg ttcttctgcc cattgctgac ggtgatggcg
     6301 atttatcgtg gtcgcccggg ttggggattt ttgatggcat cattgatgca atttggcgtg
     6361 attttcacgg caggcatcac gctgttcccc tttgtcatgc cgtcaagcgt gagtccaatc
     6421 tccagcctga cgttgtggga cagcacttcc agccagttga cgctgagtat tatgttggta
     6481 atcgtgctga tatttttgcc cattgtgttg ctctacactc tctggagcta ctacaaaatg
     6541 tgggggcgca tcacgacaga aactctccgc cgtaacaaaa acgagctgta ctaaggagca
     6601 ggaacaatgt ggtatttact ttggttcgtt ggcattttgt tgatgtgttc gctctccacc
     6661 cttgtgttgg tatggctgga cccgcgattg aaaagttaac gaacgtaagt ctgatgcggc
     6721 gcattagcgt cgtatcaggc aatcaataat gtcggatatg aaaagcggaa acatatcgat
     6781 gaaagcgatc ttgatcccat ttttatctct tctgattccg ttaaccccgc aatctgcatt
     6841 cgctaagagt gagccggagc tgaagctgga aagtgtggtg attgtcagtc gtcatggtgt
     6901 gcgtgcccca accaaggcca cgcaactgat gcaggatgtc accccagacg catggccaac
     6961 atggccggta aaactggggt ggctgacacc gcgcggtggt gagctaatcg cttatctcgg
     7021 acattaccaa cgccagcgtc tggtggctga cggattgctg gcgaaaaagg gctgcccgca
     7081 gcctggtcag gtcgcgatta ttgctgatgt cgacgagcgt acccgtaaaa caggcgaagc
     7141 cttcgccgcc gggctggcac ctgactgtgc aataaccgta catacccagg cagatacgtc
     7201 cagtcccgat ccgttattta atcctctaaa aactggcgtt tgccaactgg ataacgcgaa
     7261 tgtgactgac gcgatcctca gcagggcagg agggtcaatt gctgacttta ccgggcatcg
     7321 gcaaacggcg tttcgcgaac tggaacaggt acttaatttc ccacaatcaa acttgtgcct
     7381 taaccgtgag aaacaggaca aaagttgttc attaacgcag gcattaccat cggaactcaa
     7441 ggtgagcgcc gacaatgtct cattaaccgg tgcggtaagc ctcgcatcaa tgctgacgga
     7501 gatatttctc ctgcaacaag cacagggaat gccggagccg gggtggggaa ggatcaccga
     7561 ttcacaccag tggaacacct tgctaagttt gcataacgcg caattttatt tgctacaacg
     7621 cacgccagag gttgcccgca gccgcgccac cccgttatta gatttgatca agacagcgtt
     7681 gacgccccat ccaccgcaaa aacaggcgta tggtgtgaca ttacccactt cagtgctgtt
     7741 tatcgccgga catgatacta atctggcaaa tctcggcggc gcactggagc tcaactggac
     7801 gcttccagct cagccggata acacgccgcc agggggtgaa ctggtgtttg aacgctggcg
     7861 tcggctaagc gataacagcc agtggattca ggtttcgctg gtctttcaga ctttacagca
     7921 gatgcgtgat aaaacgccgc tatcattaaa tacgccgccc ggagaggtga aactgaccct
     7981 ggctggatgt gaagagcgaa atgcgcaggg catgtgttcg ttggccagtt ttacgcaaat
     8041 cgtgaatgaa gcacgcatac cggcgtgcgc tctccatcaa gacaaataaa catttgccgc
     8101 tgttggtcct cgcaggccat ttacgcggca aaattccacg cgtaatcctg gtataagcac
     8161 ttccgcgtcg cggggagtga atgcggaaat atggacctgg acttctttac gaccgtcgga
     8221 ggggataatg aatcctttgc cgcttttgcg atcaaaggtt ttgacaattc ctgtcatttt
     8281 acgggacaaa caaattcctt actgaaaata ctgcgctgca ctatacgggg ttaataaaat
     8341 aaagccagcg atatttaaga ccgccggacg gctaaaataa aaattgctta atctcaatta
     8401 tcatgcgtta atagctgcgt cggtttgaaa gacagacagc atacaaagta gtttactaaa
     8461 gcagttctca ttatcaggca ttatcccctt cttttgagtc tctctcctga acactaagta
     8521 gtttctgtat taaagccctg tttgccgaaa ggcccaaaat gaaggaagta aaatatgtct
     8581 aataaaatga ctggtttagt aaaatggttt aacgcagata aaggttttgg ctttatcact
     8641 cctgatgatg gcagcaaaga cgttttcgtc catttcaccg ccatccagag caatgaattc
     8701 cgcacgctga acgaaaatca gaaagttgaa ttttctattg agcaggggca acgtggtccc
     8761 gcggcagcga acgttgttac gctctaaggt tgccgttatt actcaacatc tccatttccg
     8821 ctgtccatgt tgtcatggtt cacagtaccg cacatcggca ttcgatgtga cggagcgaaa
     8881 tcctttaggg gcaaagtgta ttttttgtaa atcgacgatg atcacctttg ataacgtcgc
     8941 gctgcaaata cgcactgagc atgcgccgct ggatttcaca aaataatatc aggctccctc
     9001 gtggagcctt ttttatatct gccttatttt tcttcaacgc tgtatgtata gtaagcgata
     9061 acctgttgat tattgaatct ttcggggaga tggcttataa catttcttac ctgaccaggg
     9121 taccgggaac caacacctta ctggcgtgtt gctgtctttc aagaccagac gaggttaaca
     9181 gtgaatattg aagagttaaa aaaacaagcc gaaacggaaa tcgccgactt tatcgcgcaa
     9241 aaaatcgccg agctgaacaa gaatacaggg aaagaagtct ctgaaattcg cttcaccgca
     9301 cgggaaaaaa tgaccgggct tgaaagttat gatgtcaaaa tcaaaataat gtgattttgt
     9361 gaacatcacc ccgtgcgagg tgatgttccg cttgttgcta atttagtgac caatcattgg
     9421 cgcttgtgga attatgcgtc ggtacaattc ctgcggtacc gggctttgcc atactcccgc
     9481 atacattgcg taaccaatca ccgcaaacat aatccccaga accagtagtg tcattaacca
     9541 gccagacaac gcaaaagctt ttttatttgc cgcaggtttt tgcagtgaaa aggtcaatgt
     9601 tgaggctacc ggacatgact ctacgcaagt catacagccg gtacattcca ctgttcgtac
     9661 ctgaattaat ttatcgaccg ggatccgcga tgggcaattt tttgcgcatt tgccacagtc
     9721 gatacaactt tcggcattgc gacgaatctt aaacggtgat aatagcgaaa ccacgcccat
     9781 cagcgcgcca tatgggcaaa gataacgaca ccaggcatgg cgaataaaca ggctggcaat
     9841 cagcaaaacg gtcacgctga ttaatgtcgc ggtccccata tggcgaaaga aatcgagcat
     9901 tttaacgtcc atcaccacgc tgtagggcga caacataaaa tagtgaatcg cctgagcggg
     9961 catcaataac gcgatataga gaaaaaaact caacagcaaa tacttcacgc cacgcagagg
    10021 aatatccaga cagcggggaa ggacacattg ccgaccaaac agtttgttac cgagatcgcc
    10081 gattaattca gaaagcgtac caaccgggca taaccatgag caaaaggcct ttttgagtaa
    10141 tagactgatg acgataaaag cgaccaataa cagcatcgcg gcggcgtgga cggacggtaa
    10201 ctggcctgtt gcaaggctat atttcagatt catcagaccg gcaatcggta gccagccttc
    10261 gatgcctcct ggcctggcga caaatgtcgt actacctgcc gtttcgtaat agcgcaccca
    10321 ataccagaac gtgatggcaa tataaatatt cattgccaac agtaataatt gcgtcgcttt
    10381 acgccaggtc gtggcattgc gccagtcatt ccacggtaat ttgccgcccg tcgtgcctgg
    10441 ccgccgctgc cagcgggttc tttttttctc tgccatgatt ttgacagtcc gttaagttgc
    10501 ataccaaatg ccactattct agttgttctt aactggctga tattgattca aatcgcgttc
    10561 aggtctttct tattaaccat gcttccagag cggcaacact gcgtgtaatt tcttcgtgtg
    10621 gaaggggggc agataatggc tgctgctcca gttgtgcgca aagctggctg gcgctacgca
    10681 ttcccagact tgagcaactg ctttttagct gatgtgcggc acgctttatt ttttcgctgt
    10741 tctggctggc gcgggcaatg tcgatgtcat cgagaagcgg cagggcatgt tgttt
//
LOCUS       DAMZFT010000158        10727 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.158, whole genome shotgun sequence.
ACCESSION   DAMZFT010000158 DAMZFT010000000
VERSION     DAMZFT010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10727)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10727)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10727
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.158"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(149..1282)
                     /gene="thiH"
                     /locus_tag="PN977_004324"
     CDS             complement(149..1282)
                     /gene="thiH"
                     /locus_tag="PN977_004324"
                     /EC_number="4.1.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000847471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminoacetate synthase ThiH"
                     /protein_id="HDI6044285.1"
                     /translation="MKNFSDRWRQLDWDDIRLRINGKTAADVERALNASQLTRDDMMA
                     LLSPAAIGYLEPLAQRAQRLTRQRFGNTVSFYVPLYLSNLCANDCTYCGFSMSNRIKR
                     KTLDEADIARESAAIREMGFEHLLLVTGEHQAKVGMDYFRRHLPALREQFSSLQMEVQ
                     PLAETEYAELKQLGLDGVMVYQETYHEATYARHHLKGKKQDFFWRLETPDRLGHAGID
                     KIGLGALIGLSDNWRVDCYMVAEHLLWLQQHYWQSRYSVSFPRLRPCTGGIEPASIMD
                     ERQLVQTICAFRLLAPEIELSLSTRESPWFRDRVIPLAINNVSAFSKTQPGGYADNHP
                     ELEQFSPHDDRRPEAVAAALTAQGLQPVWKDWDSYLGRASQRP"
     gene            complement(1279..2049)
                     /gene="thiG"
                     /locus_tag="PN977_004325"
     CDS             complement(1279..2049)
                     /gene="thiG"
                     /locus_tag="PN977_004325"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HDI6044286.1"
                     /translation="MLHIANKTFDSHLFTGTGKFASSQLMVESIRASGSQLVTLAMKR
                     VNLRQHNDAILEPLIAAGVTLLPNTSGAKTAEEAIFAAHLAREALGTNWLKLEIHPDA
                     RWLLPDPIETLKAAETLVQQGFVVLPYCGADPVLCKRLEEVGCAAVMPLGAPIGSNQG
                     LETRAMLEIIIQQATVPVVVDAGIGVPSHAAQALEMGADAVLVNTAIAVADDPVNMAK
                     AFRLAVEAGLLARQSGPGSRSHFAHATSPLTGFLEASA"
     gene            complement(2051..2251)
                     /gene="thiS"
                     /locus_tag="PN977_004326"
     CDS             complement(2051..2251)
                     /gene="thiS"
                     /locus_tag="PN977_004326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HDI6044287.1"
                     /translation="MQILFNDQPMQCVAGLTVHELLEQLDQQQAGAALAINQQIVPRE
                     QWAQHIVQDGDQILLFQVIAGG"
     gene            complement(2235..2990)
                     /gene="thiF"
                     /locus_tag="PN977_004327"
     CDS             complement(2235..2990)
                     /gene="thiF"
                     /locus_tag="PN977_004327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418420.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole biosynthesis adenylyltransferase ThiF"
                     /protein_id="HDI6044288.1"
                     /translation="MNDRDFMRYSRQILLDDIALDGQQKLLDSQVLIIGLGGLGTPAA
                     LYLAGAGVGTLVLADDDDVHLSNLQRQILFTTEDIDLPKSQVSQQRLTQLNPDIQLMA
                     LQQRLTGETLKDAVAQADVVLDCTDNMATRQEINATCVALNTPLITASAVGFGGQLMV
                     LTPPWEQGCYRCLWPDNQEPERNCRTAGVVGPVVGVMGTLQALEAIKLLSGIETPAGE
                     LRLFDGKSSQWRSLTLRRASGCPVCGGCNADPV"
     gene            complement(2983..3618)
                     /gene="thiE"
                     /locus_tag="PN977_004328"
     CDS             complement(2983..3618)
                     /gene="thiE"
                     /locus_tag="PN977_004328"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HDI6044289.1"
                     /translation="MYQHEFPPVPFRLGLYPVVDSVQWIERLLDAGVRTLQLRIKDRR
                     DEEVEADVVAAIALGRRYNARLFINDYWRLAIKHQAYGVHLGQEDLQATDLSAIRAAG
                     LRLGVSTHDDMEIDVALAARPSYIALGHVFPTQTKQMPSAPQGLEQLARHVERLVDYP
                     TVAIGGISLPRAPEVIATGVGSIAVVSAITQAADWRLATAQLLEIAGVGDE"
     gene            complement(3618..5513)
                     /gene="thiC"
                     /locus_tag="PN977_004329"
     CDS             complement(3618..5513)
                     /gene="thiC"
                     /locus_tag="PN977_004329"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HDI6044290.1"
                     /translation="MSATKLTRREQRAQAQHFIDTLEGTAFPNSKRIYITGTQPGVRV
                     PMREIQLSPTLIGGSKEQPQYEENEAIPVYDASGPYGDPQIAINVQQGLAKLRQPWID
                     ARGDTEELTVRSSDYTKARLADDGLDELRFSGVLTPKRAKAGRRVTQLHYARRGIITP
                     EMEFIAIRENMGRERIRSEVLRHQHPGMSFGARLPENITAEFVRDEVAAGRAIIPANI
                     NHPESEPMIIGRNFLVKVNANIGNSAVTSSIEEEVEKLVWSTRWGADTVMDLSTGRYI
                     HETREWILRNSPVPIGTVPIYQALEKVNGIAEDLTWEAFRDTLLEQAEQGVDYFTIHA
                     GVLLRYVPMTAKRLTGIVSRGGSIMAKWCLSHHQENFLYQHFREICEICAAYDVSLSL
                     GDGLRPGSVQDANDEAQFAELHTLGELTKIAWEYDVQVMIEGPGHVPMQMIRRNMTEE
                     LEQCHEAPFYTLGPLTTDIAPGYDHFTSGIGAAMIGWFGCAMLCYVTPKEHLGLPNKE
                     DVKQGLITYKIAAHAADLAKGHPGAQIRDNAMSKARFEFRWEDQFNLALDPFTARAYH
                     DETLPQESGKVAHFCSMCGPKFCSMKISQEVRDYAAAQTIEVGMADMSENFRARGGEI
                     YLRKEEA"
     regulatory      complement(5594..5694)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(5746..6222)
                     /gene="rsd"
                     /locus_tag="PN977_004330"
     CDS             complement(5746..6222)
                     /gene="rsd"
                     /locus_tag="PN977_004330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma D regulator"
                     /protein_id="HDI6044291.1"
                     /translation="MLNQLDNLTERVRGSNKLVDRWLHVRKHLLVAYYNLVGIKPGKE
                     SYMRLNEKALDDFCQSLVDYLSAGHFSIYERILHKLEGNGQLARAAKIWPQLEANTQQ
                     IMDYYDSSLETAIDHDNYLEFQQVLSDIGEALEARFVLEDKLILLVLDAARVKHPA"
     gene            6317..7090
                     /gene="nudC"
                     /locus_tag="PN977_004331"
     CDS             6317..7090
                     /gene="nudC"
                     /locus_tag="PN977_004331"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HDI6044292.1"
                     /translation="MDRIIEKLDHGWWVVSHEQKLWLPKGELPYGEAANFDLVGQRAL
                     QIGEWQGEPVWLVQQQRRHDMGSVRQVIDLDVGLFQLAGRGVQLAEFYRSHKYCGYCG
                     HEMYPSKTEWAMLCSHCRERYYPQIAPCIIVAIRRDDSILLAQHTRHRNGVHTVLAGF
                     VEVGETLEQAVAREVMEESGIKVKNLRYVTSQPWPFPQSLMTAFMAEYDSGEIVIDPK
                     ELLEANWYRYDDLPLLPPPGTVARRLIEDTVAMCRAEYE"
     gene            7130..8194
                     /gene="hemE"
                     /locus_tag="PN977_004332"
     CDS             7130..8194
                     /gene="hemE"
                     /locus_tag="PN977_004332"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HDI6044293.1"
                     /translation="MTELKNDRYLRALLRQPVDVTPVWMMRQAGRYLPEYKATRAQAG
                     DFMSLCKNAELACEVTLQPLRRYPLDAAILFSDILTVPDAMGLGLYFEAGEGPRFTSP
                     VTCKADVDKLPIPDPEDELGYVMNAVRTIRRELKGEVPLIGFSGSPWTLATYMVEGGS
                     SKAFTVIKKMMYADPQALHALLDKLAKSVTLYLNAQIKAGAQAVMIFDTWGGVLTGRD
                     YQQFSLYYMHKIVDGLLRENDGRRVPVTLFTKGGGQWLEAMAETGCDALGLDWTTDIA
                     DARRRVGNKVALQGNMDPSMLYAPPARIEEEVATILAGFGHGEGHVFNLGHGIHQDVP
                     PEHAGVFVEAVHRLSEQYHR"
     gene            8204..8875
                     /gene="nfi"
                     /locus_tag="PN977_004333"
     CDS             8204..8875
                     /gene="nfi"
                     /locus_tag="PN977_004333"
                     /EC_number="3.1.21.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease V"
                     /protein_id="HDI6044294.1"
                     /translation="MDLASLRAQQIELASSVIREDRLDKDPPDLIAGADVGFEQGGEV
                     TRAAMVLLKYPSLELVEYKVARIATTMPYIPGFLSFREYPALLAAWEMLSQKPDLVFV
                     DGHGISHPRRLGVASHFGLLVDVPTIGVAKKRLCGKFEPLSSEPGALAPLMDKGEQLA
                     WVWRSKARCNPLFIATGHRVSVDSALAWVQRCMKGYRLPEPTRWADAVASERPAFVRY
                     TANQP"
     gene            8918..9508
                     /locus_tag="PN977_004334"
     CDS             8918..9508
                     /locus_tag="PN977_004334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjaG family protein"
                     /protein_id="HDI6044295.1"
                     /translation="MLQNPIHLRLERLESWQHVTFMACLCERMYPNYAMFCQQTGFGD
                     GQIYRRILDLIWETLTVKDAKVNFDSQLEKFEEAIPSADDFDLYGVYPAIDACVALSE
                     LVHSRLSGETLEHAVEVSKTSITTVAMLEMTQAGREMSDEELKENPAVEQEWDIQWEI
                     FRLLAECEERDIELIKGLRADLREAGESNIGIIFQQ"
     gene            9695..9967
                     /gene="hupA"
                     /locus_tag="PN977_004335"
     CDS             9695..9967
                     /gene="hupA"
                     /locus_tag="PN977_004335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017460010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein HU-alpha"
                     /protein_id="HDI6044296.1"
                     /translation="MNKTQLIDVIAEKAELSKTQAKAALESTLAAITESLKEGDAVQL
                     VGFGTFKVNHRAERTGRNPQTGKEIKIAAANVPAFVSGKALKDAVK"
     gene            9980..10675
                     /locus_tag="PN977_004336"
     CDS             9980..10675
                     /locus_tag="PN977_004336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1481 domain-containing protein"
                     /protein_id="HDI6044297.1"
                     /translation="MNSFNEGVVSPLLSFWRRSLMLAGALFLTACSHNSSLPPFTASG
                     FAEDQGAVRIWRKDSGDNVHLLAVFSPWRSGDTTTREYRWQGDNLTLININVYSKPPV
                     NIRARFDDRGDLSFMQRESDGEKQQLSNDQIDLYRYRADQIRQISDALRQGRVVLRQG
                     RWHAMEQTVTTCEGQTIKPDLDSQAIAHIERRQSRSSVDVSVAWLEAPEGSQLLLVAN
                     SDFCRWQPNEKTF"
BASE COUNT         2428 a         2887 c         2944 g         2468 t
ORIGIN      
        1 aggcagggcg aaacgaggaa gaagcttttc gccctggaca acattcctgc tgacggcact
       61 accataaaaa aacacaacgc tttcaacaac cttgcgtttt tcatcttccg tagcggctcg
      121 gaataaattt acgttcttac attctgtctc atggcctttg cgaggcgcgt cccagatagc
      181 tgtcccagtc tttccatacc ggctgcaaac cctgagcggt taacgcggca gcaaccgctt
      241 ccggcctgcg atcgtcgtgt ggtgagaact gttccagctc ggggtgattg tcggcatagc
      301 cacctggctg cgttttcgaa aaagcgctga cgttattaat tgccagcgga ataacgcgat
      361 cgcgaaacca cggtgattcc cgcgtggaga gtgacagttc aatctccggt gcaagcagcc
      421 ggaaggcgca gatggtttgc actaactggc gttcatccat aatcgacgca ggctcaatgc
      481 cgccagtaca tggacgcagg cgtgggaagg agacagagta gcggctttgc cagtaatgtt
      541 gttgcagcca tagcaaatgt tcggcaacca tatagcagtc aactcgccag ttgtcggaaa
      601 ggccgattag cgcgccgagg cctatcttat caatccccgc atgccctagc cgatccggcg
      661 tttccagccg ccagaagaag tcctgttttt tacctttcag atgatggcgg gcataagtcg
      721 cctcgtgata tgtctcctga taaaccatca cgccatccag acctagttgc tttaactcgg
      781 cgtattccgt ctccgccagc ggttgcactt ccatctgtag tgaagagaac tgttcacgca
      841 gggcagggag atgacgacga aagtaatcca tccccacttt cgcctgatgt tcaccagtga
      901 ctaaaagcag atgttcaaag cccatctccc gtatagcggc gctttccctg gcaatatccg
      961 cttcatccag cgttttgcgc ttgatgcggt tgctcataga gaatccgcag tacgtgcagt
     1021 cgttagcgca aagattggaa agataaagcg ggacgtagaa gctaacagtg ttgccaaatc
     1081 gttgacgggt cagacgctgc gcccgttggg ccagtggttc cagatagcca atggcggctg
     1141 gcgataacag cgccatcata tcgtcgcggg tgagttgcga ggcatttagc gcccgctcta
     1201 cgtcagcagc cgttttgccg ttgatacgca ggcggatgtc gtcccagtcc agttgtcgcc
     1261 agcgatcgct gaagtttttc atgccgatgc ctccagaaat ccggtcagcg ggctggtggc
     1321 atgagcaaaa tgactgcggc tgcccggtcc ggactgacgt gccagcaggc ctgcttctac
     1381 tgccagacga aatgccttcg ccatgttgac gggatcgtcc gcgacggcaa tcgccgtatt
     1441 cactaacacc gcgtcggccc ccatttccag cgcctgcgcg gcatggctgg gaacgccgat
     1501 gccagcatcg acaaccaccg gaaccgtggc ttgctggata ataatctcca gcatggcacg
     1561 ggtttccagt ccctgattcg agccaatcgg cgcgccgagc ggcatcaccg ctgcacagcc
     1621 gacttcttcc agacgtttac acaataccgg atcggccccg cagtaaggca gcacgacaaa
     1681 tccctgttgt accagcgttt cggcggcttt cagggtttcg atgggatcgg gcaacagcca
     1741 gcgggcgtca gggtgaatct ctaattttaa ccagtttgtg cctaacgctt cacgagccag
     1801 atgggcggcg aaaatggctt cttccgctgt tttcgccccg gatgtatttg gcagcagggt
     1861 cacacccgcc gcgataagcg gttcgaggat agcgtcgttg tgctggcgca agttgacgcg
     1921 tttcatcgcc agcgtcacca gttggctgcc ggaagcgcga atagactcca ccatcagttg
     1981 tgaagaggcg aattttccgg tgccagtaaa cagatgtgaa tcaaacgttt tgttcgcaat
     2041 atgtaacatc tcaaccccct gcaataactt gaaaaagcag gatctggtcg ccatcctgca
     2101 cgatatgttg cgcccattgt tcccgcggga cgatttgctg attaatcgcc agtgccgcac
     2161 ctgcttgttg ttgatcgagt tgctccagca gttcgtgaac agttagtccg gcgacacact
     2221 gcatcggttg atcgttaaac aggatctgca ttgcatcctc cacataccgg gcaaccgctg
     2281 gcgcggcgca acgtcagact gcgccactgg ctcgatttac cgtcgaacag tcggagttct
     2341 cccgcaggcg tctctatacc gcttaataac ttaatggctt ccagtgcctg taaagtgccc
     2401 ataaccccga ccaccgggcc aaccacgccc gccgtgcggc agttgcgttc tggctcctgg
     2461 ttatctggcc acaggcagcg gtaacacccc tgctcccagg gcggcgtcag taccatcaac
     2521 tgaccgccaa atccgaccgc gctggcggtg ataagcggcg tgttgagtgc cacgcaggtg
     2581 gcattaatct cctggcgagt cgccatattg tcagtacagt cgagcaccac atcggcttgt
     2641 gcaaccgcat cttttaacgt ctcacccgtt aatcgttgtt gtaatgccat cagttgaatg
     2701 tcgggattca actgtgtcag tcgctgttgg ctgacctgtg atttcgggag atcgatatct
     2761 tcagtggtaa agaggatttg tcgttgcaga ttgcttaaat gcacatcgtc gtcatctgcc
     2821 agtaccagcg tcccgacgcc agcccccgcc aggtatagcg cagcaggtgt acccagcccg
     2881 ccaagaccaa taatcagcac ctggctgtcg agcagttttt gctgcccgtc cagagcgata
     2941 tcgtcgagca ggatttggcg gctataacgc ataaagtcac ggtcattcat cgccaactcc
     3001 tgcaatttcc agcaactgtg ccgttgccaa acgccagtct gcggcttgag taatggcgct
     3061 gacgacggcg atactgccga cacccgttgc tatcacctca ggcgcgcgcg gcagactgat
     3121 accgccaatc gccacggtgg gataatccac caggcgctca acatgccgtg ccagctgttc
     3181 cagcccctgc ggtgcggaag gcatctgttt ggtttgtgtc ggaaacacat gtcccagcgc
     3241 gatataagaa ggacgtgccg ccagcgcgac gtcgatttcc atatcatcat gtgtcgaaac
     3301 gcccagtcgc agacctgccg cgcggatggc gctgagatcg gtggcttgca aatcttcctg
     3361 ccccaaatgg acgccgtatg cttgatgctt gatcgccagt cgccagtaat cattaataaa
     3421 caatcgcgcg ttatatcggc ggcccagcgc aattgccgcc acgacatcgg cttctacctc
     3481 ttcatcgcgc cgatctttga tgcgtagctg gagagtacgt acgcctgcat ccaacagacg
     3541 ttcgatccac tgtacgctgt caaccaccgg gtacagtcct aaacgaaagg gtacaggagg
     3601 aaactcatgc tgatacatca cgcttcctcc ttacgcaggt agatttctcc gcctctggcg
     3661 cggaagttct ccgacatatc cgccattccc acttcaatgg tttgcgcggc ggcgtaatca
     3721 cgcacttcct ggctgatttt catcgagcag aatttcggtc cacacatgga gcaaaaatgg
     3781 gcgactttac cggactcttg cggcagggtt tcatcgtgat aagcgcgggc agtaaacggg
     3841 tcgagggcca gattaaactg gtcttcccag cgaaattcga agcgggcttt cgacatggcg
     3901 ttatcgcgaa tttgcgcgcc cggatgccct ttcgccaggt cggcggcgtg agcggcaatc
     3961 ttataggtga taagcccttg tttaacatct tctttatttg gcaggcccag atgctctttc
     4021 ggcgttacgt aacagagcat cgcgcagcca aaccagccaa tcatcgccgc accaatcccc
     4081 gacgtgaagt ggtcataacc cggcgcgata tcggtagtta gcggccccag agtgtaaaac
     4141 ggcgcttcgt ggcattgctc taactcctcg gtcatattgc ggcggatcat ctgcatcggc
     4201 acgtggcctg ggccttcaat catcacctgt acgtcatatt cccaggcgat tttggtcagt
     4261 tcacccagcg tatgcagctc ggcaaattgc gcttcatcgt tggcgtcctg aacagaaccg
     4321 gggcgcagac cgtcgcccag cgacagcgaa acgtcataag cggcacagat ttcacaaatt
     4381 tcgcggaagt gttgatagag gaaattttcc tgatgatggg agaggcacca tttcgccata
     4441 atcgaaccgc cgcgagagac gataccagtc aggcgtttcg cggtcatcgg cacatagcgc
     4501 agcagtacgc ccgcatggat agtgaagtaa tccacacctt gctcggcttg ttccagcagc
     4561 gtgtcgcgga acgcttccca ggtaagatct tcggcgatcc cgttaacctt ctccagcgcc
     4621 tggtagatcg gcactgtacc gatcggcacc gggctgttac gcaaaatcca ctcgcgggtt
     4681 tcgtgaatat agcgaccggt ggagagatcc atcaccgtat ccgctcccca gcgcgtggac
     4741 cataccagct tttccacttc ttcttcgatg gaagaggtga ccgccgagtt gccgatattg
     4801 gcgttaactt ttaccaggaa attgcgacca ataatcatcg gctccgattc cggatgatta
     4861 atgttggccg ggataatcgc acgtccggca gcaacttcat cacggacaaa ttccgcagtg
     4921 atattttccg gcagacgtgc gccaaagctc attcccggat gctggtggcg caaaacttcg
     4981 ctacgtatgc gctcgcgacc catattctcg cgaatggcga tgaattccat ttcgggcgtg
     5041 atgatgcccc ggcgggcgta gtgcagttgg gtgacgcggc gtcctgcttt ggcgcgcttt
     5101 ggggttaata cgccgctaaa acgtagctcg tcgaggccat catctgccag tcgcgcttta
     5161 gtgtaatcgg aactgcgcac ggtaagttct tcggtatcgc cgcgcgcatc gatccacggc
     5221 tggcgtagtt ttgccagccc ttgctgcacg ttaatggcaa tctgcggatc gccatacggg
     5281 ccggaggcgt cgtagaccgg aatcgcttcg ttttcttcgt actgcggctg ttctttgcta
     5341 ccgccgatta gcgtcgggct aagctggatc tcacgcatcg gcacgcgcac gccgggttgt
     5401 gtgccggtga tgtaaatgcg ttttgagttt ggaaaggcgg tgccttccag ggtgtcgata
     5461 aaatgttggg cctgggcgcg ttgttcgcgg cgggtcagtt ttgttgcaga catagctcat
     5521 tccaaaaagt taaggacgtg gcttgtcaga cgacggatga agcaagagac gatcgccgca
     5581 aaggcgatgc gatagcagat taactcttgt tcccttcgca ggtattagcc tgatcaggtt
     5641 ccgcggatcc cgaataaacg gtctcagcca gttaaggcac tccgacaaga aattagcccc
     5701 acaaacgggg cgttgaatgt aaattacgcg ttaacagcgc agaactcaag caggatgttt
     5761 gacgcgggcg gcgtcaagca ccagcagaat cagcttatct tccagcacaa agcgcgcttc
     5821 cagcgcctcg ccgatgtcag ataaaacctg ttgaaactca aggtaattat catgatcgat
     5881 ggcggtttcc aggctggaat cgtagtaatc catgatctgt tgggtattgg cttcgagttg
     5941 cggccaaatc ttcgcggcgc gcgcgagttg cccgttgcct tccagcttat gaagaatgcg
     6001 ctcataaata ctgaaatgtc cggcagacaa gtaatcgacc aggctctgac aaaaatcatc
     6061 aagggctttt tcgtttagcc tcatgtacga ttctttgcca ggcttaatgc caaccagatt
     6121 gtagtaagcc acgagcagat gcttacgtac atgtagccag cgatcaacca gtttgttact
     6181 tcctctgacg cgctccgtca ggttatcgag ctggttaagc atgattgact ccgcaagttt
     6241 gtattcaaaa actgctcagt gagaaatgta aaaaccatgt taaacatgcc agtgatgcaa
     6301 aggtagtgca agagctatgg atcgtataat tgaaaaatta gatcacggct ggtgggtcgt
     6361 cagccatgaa caaaaattat ggttgccgaa gggagaattg ccatatggcg aagcggcaaa
     6421 tttcgatctt gtgggtcagc gcgcactaca aatcggcgaa tggcaggggg aacctgtttg
     6481 gttagtacaa cagcagcggc gtcacgatat ggggtcggta cgtcaggtca ttgatctcga
     6541 tgttgggctg tttcaactgg ccggacgtgg cgtgcaactg gcggagtttt accgatcgca
     6601 taaatactgt ggttactgcg ggcatgaaat gtatccaagc aaaaccgaat gggcgatgct
     6661 gtgcagccat tgccgtgagc gttactaccc gcaaatcgcc ccctgcatta ttgttgccat
     6721 ccgtcgcgat gattcgatcc tcctcgccca gcatacccgc catcgtaacg gtgtccatac
     6781 ggtacttgcc ggattcgtcg aagtgggcga aaccctcgag caggctgtcg cgcgggaagt
     6841 gatggaagag agcggaatta aagttaaaaa cttgcgttac gtgacttccc agccgtggcc
     6901 gtttcctcag tctttaatga ccgcgtttat ggcggaatat gacagcggcg agatcgtgat
     6961 cgacccgaaa gaattgcttg aggcgaactg gtatcgctat gacgatttgc cgttactccc
     7021 gccgcccggc accgtagcgc gccgtctgat agaagataca gtggcgatgt gtcgggcaga
     7081 gtatgagtga tgatacactg accgcctgac gcactaagga acagccaaaa tgaccgaact
     7141 taaaaacgat cgttatctgc gggcgctgct gcgccagccc gttgatgtca ctccagtatg
     7201 gatgatgcgc caggcgggtc gctatctacc ggaatataaa gccacgcgcg cccaggcggg
     7261 cgattttatg tcgctgtgca aaaacgccga gctggcgtgc gaagtgactt tgcaaccgct
     7321 gcgtcgctac ccgctggatg cggcgatcct cttttccgat atcctcaccg tgccggacgc
     7381 gatggggtta gggctctatt ttgaagccgg agaaggtccg cgttttacct cgccagtcac
     7441 ctgcaaagcc gacgtcgata aactgccaat tccggacccg gaagatgagc tgggttacgt
     7501 gatgaacgcg gtgcgtacca ttcgtcgcga actgaaaggc gaagtgccgc tgattggttt
     7561 ttctggcagc ccgtggacgc tggcgaccta catggtggaa ggcggcagca gcaaagcctt
     7621 caccgtgatc aaaaaaatga tgtatgccga tccgcaggcg ctgcacgcgc tactcgataa
     7681 actggcgaaa agcgtcactt tgtatctgaa tgcgcagatt aaagccggtg ctcaggcagt
     7741 gatgattttc gacacctggg gcggcgtgct taccgggcgc gattatcaac agttctcgct
     7801 ctattacatg cataaaattg ttgatggttt actgcgtgaa aacgacggtc gccgcgtacc
     7861 ggtcacgctg tttaccaaag gtggcggaca gtggctggaa gcgatggcag aaaccggttg
     7921 cgatgcgctg ggcctcgact ggacaacgga catcgccgat gcgcgccgtc gcgtgggcaa
     7981 taaagtcgcg ctgcagggta atatggatcc gtcgatgctg tacgcgccac ctgcccgcat
     8041 tgaagaagaa gttgcgacta tacttgccgg tttcggtcac ggcgaaggtc atgtctttaa
     8101 ccttggtcac ggcattcatc aggatgtgcc gccagaacat gctggcgtgt ttgtggaggc
     8161 agtgcatcgc ttgtctgaac agtatcaccg ctaaggagcg attatggatc tcgcgtcatt
     8221 acgcgctcaa caaattgaac tggcttcttc tgtgatccgc gaggatcgac tcgataaaga
     8281 tccgccggat ctgatcgccg gagccgatgt cggatttgag cagggcggag aagtgacgcg
     8341 agcggcgatg gtgctgctga aatacccctc gcttgagctg gtggagtata aagtggcccg
     8401 catcgccacc accatgcctt acattccagg ttttctctcc ttccgcgaat atcctgcgct
     8461 gctggcagcg tgggagatgc tgtcacaaaa gccggattta gtgtttgtcg atggtcacgg
     8521 gatctcgcat cctcgccgtc ttggcgttgc cagccacttt ggtttgctgg tggatgtgcc
     8581 gaccattggc gtggcgaaaa aacggctctg cggtaaattc gaaccgctct ccagcgaacc
     8641 gggcgcgctg gccccactga tggataaagg cgagcagctg gcctgggtct ggcgcagcaa
     8701 agcgcgctgt aacccgttgt ttatcgctac cggtcatcgg gtcagcgtgg acagcgcgct
     8761 ggcgtgggta caacgctgca tgaaaggcta tcgtctgccg gagccaacgc gctgggcgga
     8821 cgcggtggcc tcggaacgtc cggcgttcgt gcgctataca gcaaatcagc cctaattcag
     8881 gtaaactgcg gataatttcc gtatttgaga actcaacatg ttacaaaacc caattcatct
     8941 gcgtctggag cgcctggaaa gctggcagca cgtcactttc atggcttgct tatgcgaacg
     9001 catgtacccc aattacgcca tgttctgcca gcaaaccggt tttggtgatg ggcaaattta
     9061 ccgccgcatt ctcgatctca tctgggaaac gctgaccgtc aaagacgcaa aagtaaattt
     9121 cgacagccaa ctggagaaat ttgaagaagc gattccttca gccgacgatt tcgatctgta
     9181 cggcgtttat ccggcaatcg atgcatgcgt ggcgttaagt gaactggtcc attcgcgttt
     9241 aagtggcgaa acgctcgaac acgcggtgga agtgagtaag acctccatca cgaccgttgc
     9301 gatgctggaa atgactcagg ctggtcgcga aatgagcgat gaagagctca aagaaaaccc
     9361 agctgtagag caagaatggg acattcagtg ggaaatattc cgacttttag ctgagtgcga
     9421 agaacgcgat atcgagctga taaaaggcct tagggcagac ctgcgtgagg cgggtgagag
     9481 caatattggt ataatttttc agcaataaga ccagaaaacg tgatttaacg cctgatttgt
     9541 cgtacctgga gtcttccctt tcgccccccg tctggtctac atttgggggg cgaaaaaaag
     9601 tggctatcgg tgcgtgtatg caggagagtg ctattctggc atttccgtcg cactcgatgc
     9661 ttagcaagcg ataaacacat tgtaaggata acttatgaac aagactcaac tgattgatgt
     9721 aattgcagag aaagcagaac tgtccaaaac ccaggctaaa gctgctctgg agtccactct
     9781 ggctgcaatt actgagtctc tgaaagaagg cgatgctgta caactggttg gtttcggtac
     9841 cttcaaagtg aaccaccgcg ctgagcgtac tggccgcaac ccgcagaccg gtaaagaaat
     9901 caaaattgcc gcagctaacg taccggcatt tgtttctggc aaggcactga aagacgcagt
     9961 taagtaagat tgcgtggcag tgaacagttt taacgaaggg gtggtttcac cccttttgtc
    10021 tttctggcgt cgatcattga tgctggctgg cgctctgttt ctcactgcct gtagtcacaa
    10081 ctcttcactt cctcccttta ccgccagtgg atttgctgaa gaccagggcg cggtacgcat
    10141 ctggcgaaaa gacagcggcg ataatgtgca tctgcttgcc gtgtttagcc cgtggcgcag
    10201 tggcgatacc acaacgcgag agtatcgctg gcagggcgat aacctcacgc tcatcaatat
    10261 caatgtttac agcaaaccgc cggtgaacat tcgcgcgcgt tttgacgatc gcggcgatct
    10321 gagctttatg caacgtgaat ccgatgggga aaagcagcag ctttctaacg accaaatcga
    10381 tttataccgt tatcgtgctg atcagatccg ccagattagc gatgccttac gtcaggggag
    10441 agtcgtgctg cgccaggggc gctggcatgc gatggagcag actgtgacca cctgcgaagg
    10501 gcaaaccatt aaacctgatt tagattcgca ggcgatagcg catatcgagc gccgccagag
    10561 ccgctcttct gttgatgtca gcgtggcatg gctggaagcg cccgaaggtt cgcaattact
    10621 gttggtggca aactctgatt tctgtcgctg gcaacccaac gagaaaacgt tctgatttac
    10681 cagtggccca tacccatatg accgccacca tcgcaaccac cgtaacc
//
LOCUS       DAMZFT010000159        10680 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.159, whole genome shotgun sequence.
ACCESSION   DAMZFT010000159 DAMZFT010000000
VERSION     DAMZFT010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10680)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10680)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10680
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.159"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(<1..572)
                     /gene="ygeA"
                     /locus_tag="PN977_004337"
     CDS             complement(<1..572)
                     /gene="ygeA"
                     /locus_tag="PN977_004337"
                     /EC_number="5.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HDI6044298.1"
                     /translation="MKTIGLLGGMSWESTIPYYRLINEGIKQRLGGLHSAQVLLHSVD
                     FHEIEECQRRGEWDKTGDILAEAALGLQRAGAEGIVLCTNTMHKVADAIESRCSLPFL
                     HIADATGRAITGAGMTRVALLGTRYTMEQDFYRGRLTEQFSINCLIPEADERAKINQI
                     IFEELCLGQFTEASRAYYAQVIARLAEQGA"
     gene            complement(701..2119)
                     /gene="araE"
                     /locus_tag="PN977_004338"
     CDS             complement(701..2119)
                     /gene="araE"
                     /locus_tag="PN977_004338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-proton symporter AraE"
                     /protein_id="HDI6044299.1"
                     /translation="MVTINTESALTPRSLRDTRRMNMFVSVAAAVAGLLFGLDIGVIA
                     GALPFITDHFVLTSRLQEWVVSSMMLGAAIGALFNGWLSFRLGRKYSLMAGAILFVLG
                     SIGSAFATSVEMLIAARVVLGIAVGIASYTAPLYLSEMASENVRGKMISMYQLMVTLG
                     IVLAFLSDTAFSYSGNWRAMLGVLALPAVLLIILVVFLPNSPRWLAEKGRHIEAEEVL
                     RMLRDTSEKAREELNEIRESLKLKQGGWALFKINRNVRRAVFLGMLLQAMQQFTGMNI
                     IMYYAPRIFKMAGFTTTEQQMIATLVVGLTFMFATFIAVFTVDKAGRKPALKIGFSVM
                     ALGTLVLGYCLMQFDNGTASSGLSWLSVGMTMMCIAGYAMSAAPVVWILCSEIQPLKC
                     RDFGITCSTTTNWVSNMIIGATFLTLLDSIGAAGTFWLYTALNIAFVGITFWLIPETK
                     NVTLEHIERKLMAGEKLRNIGV"
     gene            complement(2434..3195)
                     /gene="kduD"
                     /locus_tag="PN977_004339"
     CDS             complement(2434..3195)
                     /gene="kduD"
                     /locus_tag="PN977_004339"
                     /EC_number="1.1.1.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-D-gluconate 5-dehydrogenase
                     KduD"
                     /protein_id="HDI6044300.1"
                     /translation="MILSAFSLEGKVAVVTGCDTGLGQGMALGLAQAGCDIVGINIVE
                     PTETIKQVTALGRRFLSLTADLRKIDGIPALLDRAVAEFGHIDILVNNAGLIRREDAL
                     EFSEKDWDDVMNLNIKSVFFMSQAAAKHFIAQGNGGKIINIASMLSFQGGIRVPSYTA
                     SKSGVMGVTRLMANEWAKHNINVNAIAPGYMATNNTQQLRADEQRSAEILDRIPAGRW
                     GLPSDLMGPVVFLASSASDYVNGYTIAVDGGWLAR"
     gene            complement(3225..4061)
                     /gene="kduI"
                     /locus_tag="PN977_004340"
     CDS             complement(3225..4061)
                     /gene="kduI"
                     /locus_tag="PN977_004340"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HDI6044301.1"
                     /translation="MDVRQSIHSAHAKTLDTQGLRNEFLVEKVFVADEYTMVYSHIDR
                     IIVGGIMPVTKTVSVGGEVGKQLGVSYFLERRELGVINIGGAGTITVDGQCYEIGHRE
                     ALYVGKGAKEVVFASIDTATPAKFYYNCAPAHTTYPTKKVTPDEVSPVTLGDNLTSNR
                     RTINKYFVPDVLETCQLSMGLTELAPGNLWNTMPCHTHERRMEVYFYFNMDDDACVFH
                     MMGQPQETRHIVMHNEQAVISPSWSIHSGVGTKAYTFIWGMVGENQVFDDMDHVAVKD
                     LR"
     gene            complement(4348..5529)
                     /locus_tag="PN977_004341"
     CDS             complement(4348..5529)
                     /locus_tag="PN977_004341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HDI6044302.1"
                     /translation="MKDVVIVGALRTPIGCFRGALAGHSAVELGSLVVKALIERTGVP
                     AYAVDEVILGQVLTAGAGQNPARQSAIKGGLPNSVSAITINDVCGSGLKALHLATQAI
                     QCGEADIVIAGGQENMSRAPHVLTDSRTGAQLGNSQLVDSLVHDGLWDAFNDYHIGVT
                     AENLAREYGISRQLQDAYALSSQQKARAAIDAGRFKDEIVPVMTQSNGQALVVDTDEQ
                     PRTDASAEGLARLNPSFDSLGSVTAGNASSINDGAAAVMMMSEAKARALNLPVLARIR
                     AFASVGVDPALMGIAPVYATRRCLERVGWQLADVDLIEANEAFAAQALSVGKMLEWDE
                     RRVNVNGGAIALGHPIGASGCRILVSLVHEMVKRNARKGLATLCIGGGQGVALTIERD
                     E"
     gene            5784..7013
                     /locus_tag="PN977_004342"
     CDS             5784..7013
                     /locus_tag="PN977_004342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease"
                     /protein_id="HDI6044303.1"
                     /translation="MSNIWSKEETLWSFALYGTAVGAGTLFLPIQLGSAGAVVLFITA
                     LVAWPLTYWPHKALCQFILSSKTSAGEGITGAVTHYYGKKIGNLITTLYFIAFFVVVL
                     IYAVAITNSLTEQLAKHMVIDLRIRMLVSLGVVLILNLIFLMGRHATIRVMGFLVFPL
                     IAYFLFLSIYLVGSWQPDLLTTQVEFNQNTLHQIWISIPVMVFAFSHTPIISTFAIDR
                     REKYGEHAMDKCKKIMKVAYLIICISVLFFVFSCLLSIPPSYIEAAKEEGVTILSALS
                     MLPNAPAWLSISGIIVAVVAMSKSFLGTYFGVIEGATEVVKTTLQQVGVKKSRAFNRA
                     LSIMLVSLITFIVCCINPNAISMIYAISGPLIAMILFIMPTLSTYLIPALNPWRSIGN
                     LITLIVGILCVSVMFFS"
     gene            7473..8105
                     /locus_tag="PN977_004343"
     CDS             7473..8105
                     /locus_tag="PN977_004343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDI6044304.1"
                     /translation="MGLCSRYKSLTCNSCSMHCQIMPEESPRLQYCANSCFCMWPEES
                     SYFNRGVVEGILTKNHNARLSGYIFVDFSVSFLRLFLEKDWIDYLASTDMGIVLISDR
                     NMQSLANYWRKHNSAISAVIYNDDGLDVANEKIRQLFIGRYLSFTRGNTLTQMEFTIM
                     GYMVSGYNPYQIAEVLDMDIRSIYAYKQRIEKRMGGKINELFIRSHSVQH"
     gene            8439..9248
                     /locus_tag="PN977_004344"
     CDS             8439..9248
                     /locus_tag="PN977_004344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDI6044305.1"
                     /translation="MYWIINDNIEFWPEHRKLISVHNADLNVVLTTPASRCLSLLLEA
                     FPDVVAQQDFFTRVWEEEGMRVPTNTLYQNISIIRRGFRAVGDTTHSLIATVPRRGFK
                     IHNDINIQNHAINSSTDAHTHNAPPAVKGNAGYKESIGSANNFNNKILKHIKSYLIML
                     SIFAIGVYSAHWLWNNNQPKPFFKDYKTVAEINGCHFNVTEDTIDGLKEFDKYKTRIL
                     DSGINCKKYPWLYFPLAKSSPGMIVMACNKNYNQHEVAGCLTLSYREVNRD"
     gene            9241..9723
                     /locus_tag="PN977_004345"
     CDS             9241..9723
                     /locus_tag="PN977_004345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6044306.1"
                     /translation="MIDYKKNLLFILVFISGFILFIVYSYTAEKMIYNETCTANWVIF
                     NDKGRANLTIDFMYNKKNKTGTVALSGTWQQGNRESKSIRRNIEYTWIENYDTAHLTS
                     KKVNKFEIMDQVDDDRLAELIPDFYVFPEKSVSYNILKQGKHAFILSIGNRAIMHCAR
                     "
     gene            complement(9756..9836)
                     /locus_tag="PN977_004346"
     CDS             complement(9756..9836)
                     /locus_tag="PN977_004346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6044307.1"
                     /translation="MKDVDQNFDALYWHILQAYLILLFYD"
     gene            complement(9872..10174)
                     /locus_tag="PN977_004347"
                     /pseudo
     CDS             complement(9872..10174)
                     /locus_tag="PN977_004347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417326.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            10266..10680
                     /locus_tag="PN977_004348"
                     /pseudo
     CDS             10266..10680
                     /locus_tag="PN977_004348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612175.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         3113 a         2506 c         2225 g         2836 t
ORIGIN      
        1 tgtgcgccct gttctgcaag gcgagcaatc acttgcgcat aataagcgcg tgacgcttcg
       61 gtaaattgcc ccagacacag ttcttcaaaa ataatctgat taattttcgc ccgttcatcc
      121 gcttcaggaa taagacagtt gatggaaaat tgttccgtca gccgcccgcg ataaaaatcc
      181 tgttccatgg tgtaacgcgt acccagcagc gccacacgag tcattcctgc cccggtaatt
      241 gcacgtccag tggcatccgc tatatgtaag aaaggcagag agcaacgaga ctcaatggca
      301 tccgccactt tgtgcatcgt attggtacat aacacaatgc cttctgcgcc cgcccgctgt
      361 aagccaagcg ccgcctcagc cagaatatcc ccggttttgt cccactcacc gcgacgctgg
      421 cactcttcta tttcatgaaa atcgacgcta tgtagcagca cttgcgcaga gtgaagccca
      481 ccaagccgct gtttaatgcc ttcatttatc agacgatagt aaggaatagt ggattcccag
      541 ctcattcctc ccagcaaacc aattgttttc attcctctct cctgatgtgt gttaccgcaa
      601 caaggctgaa taaggaacga gacaaacgcc tcaacggcca ggtgcctaat ctctattaac
      661 gaaaaaaggg ccggatgtac agcacatccg gcccgtgaaa tcagacgccg atatttctca
      721 acttttcgcc tgccatcagt ttgcgctcga tatgttccag cgtgacattt ttggtttccg
      781 gaatgagcca gaaagtaatg cccacaaacg caatgttcaa cgcagtgtag agccagaacg
      841 taccggcagc gccaatgcta tcaagcagtg tcaggaaggt cgcgccgata atcatattcg
      901 acacccagtt tgtcgtcgtc gaacaggtga tgccaaaatc gcggcatttc agcggctgaa
      961 tctcagagca caggatccac accactggcg cggcgctcat cgcataaccg gcaatacaca
     1021 tcatcgtcat gccaacagag agccaggaca agccactgga agccgtaccg ttatcaaact
     1081 gcatcaggca gtaacccagc accagagtgc ctaacgccat cacgctgaaa ccaattttca
     1141 gagccggttt acgccctgct ttatctaccg taaagaccgc aatgaaggtg gcgaacataa
     1201 aggtcagccc cacgaccaga gtcgcaatca tctgttgttc tgtggtcgta aagcccgcca
     1261 ttttgaagat acgcggcgcg tagtacatga tgatgttcat accggtaaac tgctgcatcg
     1321 cctgcaacaa cataccgaga aacacagcac gacggacgtt acggttgatc ttaaacagtg
     1381 cccaaccgcc ctgttttaac ttcaggcttt cacgaatttc gttgagttct tctcgcgctt
     1441 tttccgacgt atcgcgcagc atacgcaata cttcttccgc ctcaatatga cgcccctttt
     1501 ccgccagcca gcgcgggcta tttggcagga agaccaccag aataatcagc agaactgctg
     1561 gtaaagcaag aacccccaac attgcgcgcc agttaccgct ataactgaac gctgtatcgg
     1621 ataaaaacgc cagcacgatg ccgagagtga ccatcaactg gtacatgctg atcatcttac
     1681 cgcgaacgtt ttcacttgcc atttcagaaa gatacagagg agcggtgtaa gacgcgatcc
     1741 cgacagcaat gcccaacacc acacgagcgg cgattaacat ctctacgctg gtcgcaaaag
     1801 cggaccctat cgaaccgagt acaaacagga tggcccccgc catcaggctg tatttacgcc
     1861 ccaggcggaa cgacagccaa ccattaaaca gcgcaccaat tgctgcgccg agcatcatgc
     1921 tactgaccac ccattcctgc aaacgactgg tcagtacaaa gtgatcggta atgaacggca
     1981 acgctccggc gattacgccg atatcaagac caaataacaa tcctgcgacc gcagcagcta
     2041 ccgaaacaaa catattcata cgccgcgtat cccgcaaaga acgtggcgtt aaagcagatt
     2101 ccgtattgat agtaaccatt ttttcctgcc agcagagagt aagacatagt gaaaaaatac
     2161 gtgaacaatt cacacaggtg tcaggtcgga aatagcataa atatggatta aattgctgcg
     2221 acatgtcgtt atgtgatgga tattccaatt ttcaaattaa gttgaattat tgagattatt
     2281 attaaccaac taattttaaa gcagataaaa ttcataaagt tcattaattg ataattgata
     2341 tggattattt cataaccatg atatggatta tgatgatcta caggtataaa aaaccctgcc
     2401 atgcggcagg gtcataaaag taagaagaat ggattaacga gccagccaac cgccatccac
     2461 ggcaatggta taaccattca cataatctga agcactggag gcaagaaaca ctaccggccc
     2521 catcaggtca ctcggcagtc cccaacggcc agctggaatg cggtcgagaa tttccgcgct
     2581 acgttgttca tctgcccgta gttgttgagt attgttggtc gccatgtaac ccggagctat
     2641 cgcattaaca ttaatgttgt gtttagccca ttcattcgcc atcaatcgcg tcacgcccat
     2701 cacgccgctt tttgatgcgg tataagaagg cacacggatc ccgccctgga aggagagcat
     2761 tgacgcgata ttgataatct tgccgccatt gccttgcgcg ataaagtgtt tcgccgctgc
     2821 ctgagacatg aagaatacgc tcttgatatt caggttcatg acatcgtccc agtctttttc
     2881 gctgaactcg agagcgtctt cgcggcgaat caatccggcg ttattcacca ggatatcaat
     2941 atgaccaaac tccgctaccg cgcgatccag cagtgctgga atgccatcaa tctttcgcag
     3001 atcggcggtc aggcttaaaa aacgacgccc cagcgccgtg acctgcttga tggtttcagt
     3061 cggttcaacg atgttaatgc caacaatgtc acagcccgct tgcgccagcc ccaacgccat
     3121 cccctggccc agcccagtat cacaaccagt gacgaccgca actttacctt cgagagaaaa
     3181 tgcacttaaa atcataacaa taccttattc gtttatgccc acaactagcg caaatcttta
     3241 acggccacat ggtccatatc atcaaaaacc tggttttcac cgaccatgcc ccagataaag
     3301 gtataagctt tggttccgac gccggaatgg atcgaccagc tcggggagat caccgcctgc
     3361 tcgttatgca tcacaatatg acgcgtttct tgcggctgcc ccatcatgtg gaaaacgcag
     3421 gcgtcatcat ccatattgaa atagaaataa acttccatcc ggcgctcgtg ggtgtgacac
     3481 ggcatggtgt tccacaagtt acctggagcc agctccgtca gccccatact caattggcag
     3541 gtttccagca catccgggac aaaatatttg ttaatcgtgc gacggttact ggtgaggtta
     3601 tcgcctaacg tgactggaga tacttcgtct ggtgtgactt ttttggtggg atacgtcgta
     3661 tgcgcaggtg cgcaattgta ataaaacttc gccggagtag cggtatcaat actggcaaag
     3721 acaacttctt ttgcgccttt accaacatac agggcttcgc ggtgaccgat ttcatagcat
     3781 tgaccatcga cggtaatcgt accggcaccg ccaatattga taacacctaa ctcgcgacgt
     3841 tccaggaaat agcttacccc gagttgttta ccaacttccc cgccaacgga aaccgtttta
     3901 gttaccggca taatgccgcc aacaataata cggtcaatgt ggctgtaaac catggtgtac
     3961 tcatcggcga caaatacctt ttcaaccaaa aattcattgc gcaacccctg ggtatccagc
     4021 gtttttgcat gcgcactgtg gatgctctgt cttacgtcca catcaacctc cgataatcag
     4081 tgaagcgtga aaatagaaaa cgatagtgaa acgctgtttc gttttctcta taaggggatg
     4141 atatgcccac gggatagggt tttcaattaa aaataaaaca atgtttcatt tttaaggtaa
     4201 agactgaaaa aagtgcagtg tcgatcacga atatgtcatg ggaatggaaa aatcagggag
     4261 ccctgaaaat gtatgtcgac tcgccagcca ccattattca acagattaat gacggctgat
     4321 atttatcggt aaggaggaaa tgcagagcta ttcgtcacgt tcaatggtca atgccacgcc
     4381 ctggcccccg ccgatacaaa gcgtagccag tcctttgcgg gcattacgtt tcaccatttc
     4441 atgaaccaga gaaaccagga ttcggcaacc ggacgcgcct attgggtgac cgagcgcgat
     4501 cgcaccacca ttaacattga cccgacgctc atcccattca agcatcttgc caaccgaaag
     4561 cgcctgagca gcaaacgctt cattagcctc aataagatcg acatcagcca actgccagcc
     4621 gacacgttcc aggcaacggc gtgtcgcata caccggcgca attcccatta atgccggatc
     4681 tacaccaacg ctggcaaatg cgcgaatgcg ggcaagcacg ggtaaattca acgctcgcgc
     4741 tttggcttcg ctcatcatca ttaccgcagc tgcgccatcg tttatggatg atgcattacc
     4801 cgctgtcaca gaaccgagac tatcaaatga aggatttaaa cgggccaagc cttctgcact
     4861 ggcgtcagtg cgtggctgtt catcggtatc aacaaccaac gcctgaccgt tactttgggt
     4921 cattaccggg acgatttcat ctttaaatcg tccggcgtca atcgccgctc gcgctttttg
     4981 ttgcgagcta agtgcgtaag catcctgcaa ctgacggctg atgccatatt cgcgagccag
     5041 attttcggcg gtgacaccaa tatgataatc attgaaggca tcccacaagc cgtcatgcac
     5101 cagactatca accaactggc tattgccaag ctgtgcaccg gtgcggctat cagtcagaac
     5161 gtgtggtgcg cggctcatgt tttcctggcc accggcgatg acaatatccg cctcgccaca
     5221 ctggatcgcc tgagtagcca gatgcagtgc tttaagcccg gaaccgcaaa cgtcattaat
     5281 agtgattgca gaaacgctgt taggaagacc acctttaata gccgattgcc ttgccggatt
     5341 ctgccctgcc cctgcagtca acacctgacc aagaattact tcatccaccg catatgcagg
     5401 aacgccggta cgttctatta acgctttcac gaccaggctg ccaagttcca cagcggaatg
     5461 acccgctaac gcaccacgaa agcagccgat aggtgtccgt aacgccccga caatcacaac
     5521 gtctttcatc acaacctcgc accaattaac agtgcaatag taaatgattg ttaattacag
     5581 ttatcttaat tgtttaaaaa aagtgactat tatcacaaag gaaatacgcc tgagtagcag
     5641 tcagagacat aactggcacg taaggtttgc aaccactaac ccaccaatag agaggtagat
     5701 agggcgttaa tctcccatac ttaacctggt ttatggtaaa ttgccctcca ttttgtttaa
     5761 tttgtagatg atacgttcag ataatgtcta atatttggtc aaaagaagaa actctgtgga
     5821 gtttcgcgct ctacggcaca gccgttggtg caggcacgct cttccttcct attcagttag
     5881 gttcggcagg ggctgtggtc ctgtttatta ctgctctggt cgcctggcct ttaacatatt
     5941 ggccacataa agccttatgc cagttcatcc tctcatcaaa aacatcagca ggtgaaggga
     6001 taacgggcgc ggtaacacac tactatggca agaagattgg taatctgatt accacgctgt
     6061 acttcatcgc cttttttgtc gtcgtgttga tatatgcagt ggcaattacc aactcactta
     6121 cagaacagct ggcaaagcat atggttattg atcttcgcat ccgtatgttg gtgagtctgg
     6181 gggttgtatt aatcctgaat ctcatttttc tgatgggacg ccatgcaacc attcgggtaa
     6241 tgggattttt ggtattccca ttgattgcct atttcttatt tctttctatt tacctggtcg
     6301 gtagttggca acctgatcta ttaacaaccc aggtagagtt caatcagaat acccttcacc
     6361 agatatggat atcgattccc gtgatggttt tcgcttttag ccatacgccc attatttcta
     6421 cgtttgccat agacagacgt gaaaaatatg gcgaacatgc tatggataaa tgcaaaaaaa
     6481 ttatgaaagt cgcttatctc atcatctgca taagtgtatt gttctttgtc tttagctgcc
     6541 tgctttctat tccaccttcg tatattgaag cggctaaaga agaaggggtt accattttat
     6601 cggcgctttc tatgctgccg aacgccccag catggctgtc aatttccggg attattgtcg
     6661 cagtagttgc gatgtcgaaa tcattcctgg gtacgtactt tggcgttatt gaaggtgcca
     6721 cagaggtcgt caaaacaaca ttacagcagg ttggtgtaaa gaaaagtcgt gcatttaacc
     6781 gcgcactatc aattatgttg gtatcgctga ttaccttcat tgtttgttgc attaacccga
     6841 acgcgatttc gatgatttac gcgatcagcg gcccgctcat tgccatgata cttttcatca
     6901 tgcctacgct gtcaacgtat ctcatcccgg cgcttaaccc ctggcgttcc atcggaaatc
     6961 tgattacgct gatcgtgggt atcctgtgcg tatcggtaat gttctttagc taatatccgt
     7021 tgtggatggt tcaaaattgc ttcaattttg aaccattcca gttttcataa tgtgcatttg
     7081 ttattattcc gctctatcac ttctctcgtc atatcctagt attatcagtt agttaagcac
     7141 acaaagagat ctgaacatct taaataagac tatttaagat gcataactta gattcgcaag
     7201 atatcttctg gtcattcaaa aacaatttgc gatatagata ttctcatacc atgagatagt
     7261 tatctgaacc accctcaacc aggtaagatt aaatcagtga aacataatca tatttaataa
     7321 cgagaaatgc attttaaaat gtcttaatca gaattatagc aaatacagat taatcgcaat
     7381 ttttcccagc aaaattacaa aatcatggtg tattgacatc atcaataacc aatgaaatta
     7441 tgcaattata tacggatagg gaggttctta acatggggct ttgtagtcgt tataaaagtc
     7501 ttacatgcaa tagttgctca atgcattgcc aaataatgcc agaagagtca ccgcgtttgc
     7561 agtattgcgc aaactcgtgt ttttgtatgt ggcccgaaga aagttcatat tttaatcgtg
     7621 gtgtggtgga aggtatctta acaaaaaacc acaatgcgag attaagcgga tatatttttg
     7681 tcgatttttc agtaagtttt ttacgtctat tccttgaaaa agactggatt gactatcttg
     7741 caagtactga catggggatt gttttaatca gcgaccgcaa tatgcaatcg ttagctaact
     7801 actggcgaaa acacaactct gcaatatctg ctgtcatata taatgatgat ggtcttgatg
     7861 ttgccaatga aaagatcaga caactattta ttggtcgtta tttatcattt accagaggta
     7921 acacattaac tcagatggaa ttcaccataa tggggtatat ggtttctggt tataatccat
     7981 atcagattgc tgaagttctg gatatggata tccgtagcat ctacgcgtac aagcaacgaa
     8041 tcgaaaagag aatgggtggt aaaataaacg aattatttat tcgttcacat tcggtccaac
     8101 attgatactc aacaatctgc cagaatcata ctctgcttat acgggcatct attgaaatgc
     8161 ccgtatttta caaataagca gtaaccccaa gagtaaacaa catggtattt aaaaccatca
     8221 cacgaaaaat tcagaagcat tacgaaatat gaatttacaa acaatagtgg cataaatgtt
     8281 aatcatgtta atttgcgtaa agttttacgt tgcgacatta aagcctcatt tcaatcatta
     8341 tgataaatat aaaattaata tatatttatg ccgtaaatac caatatactt agcaaactat
     8401 gtgatttcca tttcgattga tttagtgtct attgacgtat gtactggatt attaacgata
     8461 atatcgagtt ctggcctgag caccgaaaat taatatcggt acataacgcc gatcttaacg
     8521 tcgttctgac aacgccagcc agtcgttgtt tatcacttct acttgaagct tttcctgatg
     8581 tggttgcaca acaagatttt ttcaccagag tctgggaaga agaaggtatg cgtgtgccaa
     8641 ctaacacgtt atatcagaac atatccatta tcagacgcgg atttcgcgct gttggtgata
     8701 ccacccactc gcttattgca accgtgccga gaagaggatt caagattcat aatgacatta
     8761 acatacaaaa tcatgcaata aactcttcaa cagacgcaca tacacacaat gccccacctg
     8821 ccgtaaaagg taatgcgggg tacaaagaga gcattggtag cgcaaacaat ttcaataaca
     8881 aaatcctcaa acatataaaa agttatctaa ttatgttgag catatttgct ataggcgtat
     8941 attctgcaca ttggctatgg aataataatc aaccgaaacc attcttcaag gattacaaga
     9001 ctgttgcgga aattaatggc tgccatttta atgtgacaga agatacaatt gatgggttga
     9061 aagagttcga taaatataag acacggatac tggattcggg gattaactgt aaaaaatatc
     9121 cgtggttata cttccctctt gcaaaatcct caccagggat gattgttatg gcatgcaata
     9181 aaaactataa ccaacatgaa gtggcaggct gtttaaccct gtcttaccgt gaggttaatc
     9241 gtgattgatt ataaaaaaaa cttattattt attttagttt ttatcagtgg gtttattctc
     9301 ttcattgttt actcttatac tgcagaaaaa atgatatata atgaaacatg cactgcaaat
     9361 tgggtaattt ttaatgacaa ggggcgagca aatcttacca tcgactttat gtataacaaa
     9421 aaaaataaaa caggaactgt tgcgttgagc ggaacctggc aacaaggtaa cagagaaagt
     9481 aaatcaatac ggcgaaatat tgaatacaca tggattgaaa actatgacac agcccattta
     9541 acatcaaaga aagttaataa atttgagatc atggatcaag tcgatgatga tagacttgca
     9601 gaacttattc ctgattttta tgtctttcca gaaaaaagtg taagctataa tatactaaag
     9661 caaggtaagc atgcttttat tttgagcatt ggtaacagag caataatgca ttgtgcaagg
     9721 taaacttaaa tagataacaa aaagatggaa cctcgttaat cataaaacaa taaaattaaa
     9781 tatgcttgta gtatatgcca gtataaagca tcaaagtttt gatcaacatc tttcatttta
     9841 gatatcacct tgcaatgtaa aatatcatgt atcaacaaca tctggtgcaa acccatgagt
     9901 cgaactcaac attcatctta gctctattat tattccttcg tacagtcgat gtaaacaata
     9961 cagaaagagg attattaaga accagtttac ttagtgaatc aatagaggaa aggactaacg
    10021 tttctttaag agaattgatt tcatcatctg ttaaactaaa ctcatcattg acagatcgtg
    10081 agatataact gtttttaact ttactcttta cgttgacttt actgacagaa ttaacattca
    10141 catatcttga atttagtacc catgatgttg tttgtagact ggccccctga atctccagac
    10201 aaccaatatc acttatttaa gtgatagtct taatactagt ttttagacta gtcattggag
    10261 agcagatgat tgatgtctta ggaccggaga aacgcagacg gcgtactaca caggaaaaga
    10321 tcgctattgt tcagcagagt tttgaaccgg gaatgacagt ctcccttgtt gcccggcaac
    10381 acggtgtggc agccagccag ttatttctct ggcgcaagca ataccaggag ggaagtctta
    10441 ctgctgtggc cgcaggagaa caggtcgttc ctgcctctga acttgctgcc gccatgaagc
    10501 agattaaaga actccagcgc ctgctcggca aaaaaacgat ggaaaatgaa ctccttaaag
    10561 aagccgttga atacgggcgt gcaaaaaagt ggatagcgca cgcgccctta ttgcccgggg
    10621 atggggagta agcttagtca gccgttgtct ccgggtgtcg cgtgcgcagt tgcacgtcat
//
LOCUS       DAMZFT010000160        10668 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.160, whole genome shotgun sequence.
ACCESSION   DAMZFT010000160 DAMZFT010000000
VERSION     DAMZFT010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10668)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10668)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10668
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.160"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            1..304
                     /locus_tag="PN977_004349"
                     /pseudo
     CDS             1..304
                     /locus_tag="PN977_004349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_025243830.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
     gene            350..1723
                     /locus_tag="PN977_004350"
                     /pseudo
     CDS             350..1723
                     /locus_tag="PN977_004350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000244358.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
     gene            complement(1880..2593)
                     /locus_tag="PN977_004351"
     CDS             complement(1880..2593)
                     /locus_tag="PN977_004351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004858832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HDI6044308.1"
                     /translation="MNTLSFLLFSEEESLFITLGKICVAFILGGIIGLERESKGKPVG
                     FKTCVIISVASCVLTIVSIQSAEYYAEISMNIRSDPMRLAAQIISGVGFLGAGVILHR
                     HDDAISGLTTAAIVWASAGVGITCGAGFYMHALVTTALFMIAIKLSYFVLFLQAKNQF
                     PGKVKVHVTLDERSGLQALIECINQQKNIIEAFTIRDVKKGRIEVNLKVVIRKKMTLP
                     ELYHSLSKLEHVCAIALEH"
     gene            complement(2657..3676)
                     /locus_tag="PN977_004352"
     CDS             complement(2657..3676)
                     /locus_tag="PN977_004352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000671481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HDI6044309.1"
                     /translation="MKFNPRKSENESMRIIIDCDPGNGIPGANIDDGLALALAIAAPQ
                     IALEMITTVAGNTPVDVGYAVARDLITQLDIPVAVYRGASRALLEDPQPWREKLDHGV
                     DQFGLRQLWSNVPAPALCQQVEPHAPEAIGELICRNPGEITLVATGPLTNVAIALQLY
                     PQIVHAVKNIVVMGGVFNVPGYLKDTNFGLDPEAAHAVLTSGAPVTLVPMDVTTQTQM
                     LHADLDRLAKTENGLSRYLAQTIRPWITYSMQTRNLPGCWIHDVLTIAWLLDPSLATT
                     AEDYLDVSLEGITRGMTCCYGRDTLRLNIGIPEPKGAQVTILQSIDNPRLISLIEHYI
                     QNYGA"
     gene            complement(3651..4682)
                     /locus_tag="PN977_004353"
     CDS             complement(3651..4682)
                     /locus_tag="PN977_004353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000194408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDI6044310.1"
                     /translation="MTQSHSQRVTRSDVAKEAGTSVAVVSYVINNGPRPVAEATRQRV
                     LQAIKKTGYRPNGIARALASGSTQTYGLVAPDISNPFIASMAHALQHEAFADGKVLLL
                     GDAGDSSCRERELINNMLHRQVDGLIYTSVDRHPYINLIQESGTPCVMLDRVDPGLNV
                     SVIQVDEQLAAMQVTQHLIDHGYRDIGIICGPREMLNTQDRIRGWQQALEASSLVVNP
                     SWIFSTNYTRAGGYEATKRMLEHQLPRALFATNEQQALGCLRALAEHGLRVPEDVALV
                     CFNATQESAYNVPSLTAVRQPVDKMARAAIDMLKNWDGEVRRVEFEFYLRTGESCGCQ
                     GHEVQPEKK"
     gene            complement(4787..6181)
                     /locus_tag="PN977_004354"
     CDS             complement(4787..6181)
                     /locus_tag="PN977_004354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004858838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MHS family MFS transporter"
                     /protein_id="HDI6044311.1"
                     /translation="MNTHSSTVSLTDNQQAAQERLETSEGRREFWRATVSCWLGTTME
                     YADFALYGLAAGIIFGDVFFPEATPVMALLSSFAAYSVGFIARPIGALLFGWIGDKHG
                     RKIVMVITIGLMGMSTMLIGLIPSYAQIGVWAPICLVILRFSQGLGAGAELSGGTVML
                     GEYAPVKRRGLVSSVIGLGSNSGTLLASLVWLIVLQMDKDDLLSWGWCIPFLCSILIA
                     AAALLIRRHIRETPVFERQKALLQAEREKVIREEKAQQQHDSRSFWKRTRAFWTMVGL
                     RIGENGPSYLAQGFIIGYVAKVLMVDKSVPTAAVLIASVLGFAIIPLAGWLSDRFGRR
                     IIYRWFCLLLILYAFPAFMLLDSREPWIVIPTIITGMGLASLGIFGVQAAWGVELFGV
                     TNRYTKMAFAKELGSILSGGTAPLIASALLSYYGHWWPIAIYFAFMAAIGLVTTFFAP
                     ETRGRDLNLPEDAI"
     gene            7130..7449
                     /locus_tag="PN977_004355"
                     /pseudo
     CDS             7130..7449
                     /locus_tag="PN977_004355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(7433..7567)
                     /locus_tag="PN977_004356"
     CDS             complement(7433..7567)
                     /locus_tag="PN977_004356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002464154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDI6044312.1"
                     /translation="MKIVQTSLSESVTQHLAERILCGELPAGSVLPGENELATVSRGG
                     "
     gene            complement(7787..9000)
                     /locus_tag="PN977_004357"
     CDS             complement(join(7787..8706,8706..9000))
                     /locus_tag="PN977_004357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612262.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEc31 family transposase"
                     /protein_id="HDI6044313.1"
                     /translation="MISSPQHKTGDLMNKKTKRTFTPEFRLECAQLIVDKGYSYRQAS
                     EAMNVGSTTLESWVRQLRRERQGIAPFATPITPDQQRIRELEKQVRRLEEHNTIFKKG
                     YRALDVRLAERFTIVARLSDSHSVVSLCSALEIHRSSYRYWRKRRDTVNPARVRLCSE
                     IRRAWNQSRGSAGARTLAEMLTQNGVPMSRYRAGRLMKYLNLSSCQPGKHQYKNARQE
                     HTCLPNLLERQFAVPEPDRVWCGDITYIWAGNRWCYLAVVMDLFARRVIGWSLSANAD
                     TALISSALRMAYEVRGQPRDVMFHSDQGSQYTGLKYQQLLWRYRIKQSVSRRGNCWDN
                     SPMERFFRSLKTEWVPTDGYTGKDVARQQISSYILNYYNSVRPHHYNGGLTPEESENR
                     YHFYCKTVASIT"
     gene            9511..9809
                     /gene="tnpB"
                     /locus_tag="PN977_004358"
                     /pseudo
     CDS             9511..9809
                     /gene="tnpB"
                     /locus_tag="PN977_004358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017901265.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
     gene            10196..>10668
                     /gene="dgoR"
                     /locus_tag="PN977_004359"
     CDS             10196..>10668
                     /gene="dgoR"
                     /locus_tag="PN977_004359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-galactonate utilization transcriptional
                     regulator DgoR"
                     /protein_id="HDI6044314.1"
                     /translation="MTLNKTDRIVITLGKQIVHGKYVPGSPLPAEAELCEEFATSRNI
                     IREVFRSLMAKRLIEMKRYRGAFVAPRNQWNYLDTDVLQWVLENDYDPRLISAMSEVR
                     NLVEPAIARWAAERATSSDLAQIESALNEMIANNQDREAFNEADIRYHEAVLQSV"
BASE COUNT         2784 a         2644 c         2628 g         2612 t
ORIGIN      
        1 tggacggcct gaaaggcttc ccggaagcga ttaatgccgt ctacccggag acgcagctcc
       61 tgttgtgcat agtacatatg gtacgcaaca gcctgcggtt agggcgtgga aagactataa
      121 agcggtaacc cgtgacctga aacaggttta ccacacagcc acggaagaag gcgcgttgca
      181 ggcgctggat gcgttcggga aggaatggga tgcacggtac ccgcagataa gtcgcagctg
      241 gcaggcaaac tggccgaatg ttgccacctt cttcgtgtac ccggaggcga gccgcaaggt
      301 tataataaat aaccgtcaat acggtgctcc gttaacgctt acgtctgtta tggtactttc
      361 tgattgttat tcatgggcta acgagcagtt tggacatgcc agtctcggtg atccgcgtcg
      421 tacacgacgc cttgtgtcac tggcctcttc tctcgctcag catgcaggtc tttctatcgt
      481 gaaatcatcc cagtccactg ctcaggttga aggtgcgtac cgcctgatgc gtaacccgtc
      541 agtgtctccg gaagccattg ccgaagccgg atttaccgcc actgcaaggg catgtgaggc
      601 acatccgctg ctcctcgcac tggaagacac caccaccatt agtttcagcc acagtacggc
      661 atttgatgac ctagggaata ccactactaa cccgaagacg cgcgggctgc tggctcactc
      721 tgtcctgatg tatgcccccg acagcgccct gccagtcggt cttatcgaac agcagcgctg
      781 gagtcgggcc acggacacct acggtgttaa gcaccagcga aaagaacgtc cctgtgaaga
      841 aaaagaaagt tatcgctggc agcaggcttc ggagcgtatg gctgaacggc tgggggagat
      901 acagaaacgg gtaatcactg tctgtggcct ggaagcggat atctggcatt acctgcatta
      961 taaagtcagc cacggacagc gcttcgtggt gcgtgctgca cagaatagcc ggcaggaaga
     1021 agcacccggc aagctcttcg aactgccgga agtcctgaca accgcatgaa gccacatgct
     1081 gaatgtgatg taaaaaggcg ggcgcgcagc ccgccaggcc cggatgttca ccagttacag
     1141 cgaagtcggc ataaaaaatc ccgacaacag cggccaggcg cttccgctca cgtatgtctg
     1201 ttgccgggag caggcagagg acggtgcctg ctggcatctg ctgacatcag aaaaagtggc
     1261 gagtgccgcc gatgcgcgac gtatcgtcag ccattacgag cgacgctggt tgatagagga
     1321 ataccacaag gcgtggaaaa gtggtggtac atgtgtggaa tcgctgcgta tgcagacccg
     1381 ggataacctt gagcgcatag tggttatcaa ggcgtttatc gcggtacggg tgctgggcct
     1441 gcggcaggga ggtatcagtg aagaaacaca aaatgacagc tgtgagaaaa tactgttgcc
     1501 gacagagtgg aaactgctgt gggttaagct ggaaggaaaa ccgcttccgt cacagacgcc
     1561 gacactgaaa tgggcctgtc tgaaactggc gaaactgagg cgatggcatg acagcaaacg
     1621 cacaggtcgc cccggttgtg tagtcatgtg ggatggctgg ttcagacttc aggatatggt
     1681 tgagggttac ctggtgatgg agtctcttga tcaggagctc tgatcaagag acagctagta
     1741 cagtaggggg taccattatt taattcctgc agtaccaatt aacacagagc attctgcctc
     1801 tgtgaacata tgatatccag acttgactaa ttgttattgt gaatgttttt attgctgttt
     1861 tagcctctaa tcgatgcttt cagtgttcca gcgcgatagc gcaaacatgt tcaagcttag
     1921 agagtgaatg gtatagttct ggtaatgtca ttttttttct aatcacgacc ttaaggttaa
     1981 cttctattct tcccttttta acatcgcgaa tagtgaacgc ttcaataata tttttttgtt
     2041 gattaataca ctctatcagg gcttgtagcc ctgatctttc gtcaagagta acatgaactt
     2101 ttactttacc cggaaattga ttcttagctt gcaaaaagag cacaaaatag ctgagtttta
     2161 ttgcaatcat gaacaaagca gtggtaacca aagcatgcat ataaaaccca gcaccacatg
     2221 taattcccac cccagccgaa gcccaaacga tagctgcagt tgttagtcca gatatagcat
     2281 cgtcatggcg atgaaggata acacctgcgc cgagaaaacc tacaccgctg ataatctgtg
     2341 ctgccagtcg catcggatcg ctacgtatat tcatcgagat ttcagcgtaa tattctgcag
     2401 actgaatcga tacaattgtc agaacacaac tggcgactga aataatgacg cacgttttaa
     2461 atccaacagg cttccctttc gattctcttt caaggccaat aatgccacca aggataaacg
     2521 cgacgcaaat tttgcccagt gtaataaaga gagattcttc ttcactgaat aataagaagg
     2581 ataacgtatt catatttcct ccggaaaaaa ctcactccaa actgtgtacg aaggtctcct
     2641 tttctgcccc atgaggctac gcgccgtagt tctggatata gtgctctatc agcgaaataa
     2701 gccgcgggtt atcgatgctc tgcagaattg tgacctgtgc accttttggt tcagggatcc
     2761 caatattgag gcgtaatgtg tcacgtccat agcaacaagt cattccgcgt gtaatgcctt
     2821 ccagagatac atccagataa tcttcagccg ttgttgcaag agagggatcc agtaaccagg
     2881 caatggttaa cacatcgtgg atccaacacc caggcagatt gcgggtttgc atagagtatg
     2941 taatccatgg tcgaatggtt tgtgccaaat aacggctaag cccgttttct gtttttgcta
     3001 gacgatccaa atcggcgtga agcatttggg tttgcgttgt cacatccatc gggaccagcg
     3061 tgactggcgc accgctggtg agcaccgcat gagccgcctc agggtccaga ccaaaatttg
     3121 tatctttcag gtagcctgga acattgaaca cgccacccat aacgacgata tttttgaccg
     3181 cgtgaacaat ctgcgggtaa agctgcaggg cgatggctac attagtgagg gggccggtag
     3241 caaccaacgt tatttcgcct ggattacgac agatgagttc acctattgct tcaggcgcat
     3301 ggggttctac ctgctggcac aatgctggag caggaacatt cgaccagagt tgccgtaaac
     3361 caaactgatc gacaccatga tccagttttt cacgccaggg ttgcggatct tccaagagcg
     3421 cacgcgaggc accacggtag accgcaacag gaatatccaa ttgtgtaatg agatctctag
     3481 cgaccgcata tcccacgtct accggcgtat ttccggcaac agtggtgatc atttccagcg
     3541 cgatttgcgg tgcggcaatg gccagcgcca gcgccagacc gtcgtcgata ttagcgccgg
     3601 gaatgccgtt ccccggatcg cagtcaataa tgatacgcat cgattcattt tcactttttc
     3661 tcgggttgaa cttcatgccc ttggcagcca cacgactctc ctgttcgtaa ataaaactca
     3721 aattcaacac ggcgaacttc tccatcccaa tttttcagca tgtcaattgc cgcacgggcc
     3781 atcttatcga caggttgtcg aacggcggtt aaagaaggga cgttataagc cgattcttgt
     3841 gttgcgttaa agcagaccag cgcaacatct tcaggaacgc gcagcccatg ttctgccaaa
     3901 gcgcgtaaac agccgagagc ctgttgttca tttgtcgcga acagggcgcg tggcagttgg
     3961 tgctcaagca tgcgttttgt cgcctcgtaa ccgccggcgc gggtatagtt ggtcgaaaaa
     4021 atccatgagg gattgaccac taacgatgag gcttccagcg cctgctgcca gccccggata
     4081 cgatcctgag tattcagcat ttcacgcggg ccgcagatga tgccgatgtc gcggtatccg
     4141 tgatcaataa gatgttgtgt tacctgcatc gccgccagtt gttcatcaac ctggataacg
     4201 ctgacgttaa gcccaggatc cacacgatcg agcatgacgc agggggtacc gctctcctga
     4261 atcaaattga tataaggatg gcgatcaaca ctggtgtaga tcagcccgtc aacctggcgg
     4321 tgtagcatat tattaatgag ttcacgttca cggcagctac tatcgcctgc gtcgcccagc
     4381 aggagtactt tgccatcagc aaaggcttcg tgttgcaggg catgagccat agaggcgata
     4441 aacgggttcg agatgtccgg agcgactagc ccataagttt gcgtactccc tgaagccagc
     4501 gcacgcgcga tgccattggg tcgataaccg gttttcttaa tggcctgtag tacacgttgt
     4561 cgggtcgctt cggcaacggg gcgcgggcca ttattaataa cgtaactaac gacagcgacg
     4621 gatgtccctg cttcttttgc cacgtcagaa cgtgttacgc gttgtgaatg tgattgagtc
     4681 acgaagcacc tacctgattg cgttcagagt gattttttcc agacagcata aggcgttatg
     4741 acccaaaaat ctttggtaaa gaacgcgcat ctcacgttct ctggccttaa attgcatcct
     4801 cgggtaagtt gagatcccgt ccgcgagtct ctggtgcaaa gaaagtggtc accagtccaa
     4861 tcgcggccat aaaggcgaaa tagatagcga ttggccacca gtgcccgtaa tacgagagta
     4921 gcgcagaggc gataagtggt gcagtcccgc cagacagaat ggaaccgagc tcttttgcaa
     4981 atgccatttt ggtataacga ttagtgacac cgaaaagctc aacgccccac gcagcctgaa
     5041 caccaaaaat acccagtgaa gccagcccca tcccggtaat gatcgtcggg ataacaatcc
     5101 acggctcacg agaatccagc aacataaatg ccggaaaggc atacaggatc agtaacaagc
     5161 agaaccaacg atagatgata cgtctaccga atctatcgga cagccaaccc gccagaggaa
     5221 taatggcaaa tcccagaacg gatgcaataa gtacagctgc cgtgggtacg gacttatcca
     5281 ccatcagtac tttcgcgaca tagccaatga taaatccctg agcgagataa gaaggaccat
     5341 tctctcctat gcgtaatccg accatggtcc agaaggcacg ggtccgtttc cagaagctac
     5401 gactgtcatg ttgttgctgt gctttttcct cacgaataac cttttctcgt tcagcctgca
     5461 gaagggcttt ttgacgttca aagactggtg tttcgcgtat atgacgacga attaatagag
     5521 ctgcagcagc aataagaatg ctgcaaagaa aaggaataca ccatccccag cttaataagt
     5581 catccttgtc catttgcagg acgatgagcc aaaccagcga agccagtaat gttccgctgt
     5641 tcgaacccag accaataaca gatgaaacca gtccacgtcg tttaacggga gcatattcac
     5701 caagcatcac agtaccgcct gaaagttccg ctcctgctcc cagcccctga gaaaatcgaa
     5761 ggataaccag acatatcggt gcccagacgc ctatctgggc gtaactgggg attaatccta
     5821 ttagcatggt cgacatgccc atcaatccaa tagtgataac catgacaatt ttccgaccat
     5881 gtttatcgcc tatccagccg aatagtaatg caccaatagg gcgggcaata aacccaacag
     5941 aataggcggc aaagctggaa agtaatgcca tgacgggtgt cgcctccgga aaaaagacat
     6001 cgccaaagat aatacctgcg gcaaggccgt acagggcaaa atcggcatat tccatcgtgg
     6061 tgcctaacca gcaggaaact gttgctcgcc aaaactcgcg gcgtccctcg gaggtttcca
     6121 gccgctcctg agctgcctgt tggttatctg tcagggaaac agtggatgaa tgcgtgttca
     6181 taagaactcc ttgatattat ccgtcagata tacatcacag gtagatatct ttcttaaatc
     6241 tactcatgta gatattgtgt tttattacat ataaataaat ctactcgcgt agataaacca
     6301 ttcaatggaa gtaaaatcgt gatggagatc actcataagg tgtaagcgca aagctaacgt
     6361 tgtctgttac ctcgctccta gcgagctaaa ttacgcttaa aatatcagca atacacgagc
     6421 gccttgctca tttgagcagg gtgttttctt ttgtcttaca tacagtcatg accatgaaat
     6481 tagtcaacta ctcgggcagc gcgtttatgg gccgtgagta acgaagttgg atgcaaatgt
     6541 cagatcgtgt gcgcctgtta gggcgcggct ggtaagagag ccttataggc gcatttgaaa
     6601 aacctccggc tatgccggag gatatttatt ttgctaccac agtgacagtc actgctggaa
     6661 aatatacctc tgttcaaaac agtcctgttg tggatccgaa aaatacacat cgctcccgtt
     6721 atctgtggct gtatgattac catcactgag taaaaaaaat ccccaggatg caggggacgg
     6781 aaggtaaact atgctgaatc ttgtctgttt gttttaatat ttatctatcc gtcttgccaa
     6841 cggacaaaga aaaaataaca cgacgcagaa caaaagaaaa tattattttc atatcttccg
     6901 gaagactatg gcctcttcca gataattacc ttatggttgg catcaatcaa tgattcactc
     6961 tcgttctgcc tcggaactct atatggatgt acaatcatat aatgttctaa tattcacgag
     7021 atgtgaataa acaaatccac ttaagaaacg ggggggtatt taattaataa ctaagtgaag
     7081 cgccccggaa atcttggaga ctaaattccc tgagaaagag gtaaacagga tggctaaaaa
     7141 tactcgtttt tcccccgaag tccgtcaacg gacagttcgt atggttctgg aaaatcaggg
     7201 cgaatatgac tcccaatggg cagcaatttg ttccatcgtc ccaaagattg gctgtacacc
     7261 ggagactctg cgtgtctggt gtacaaccag catgagtggg ataccggggg cggtgatggt
     7321 ggactcacca ccgctgaacg tcagcgtctg aaagacgctg gagcgtgaaa atcgtgaact
     7381 gcgccacagt aacgatatcc ttcgccaagc tcttattttg cgaaggcgga gttcaaccac
     7441 ccctggaaac tgttgccagt tcattttcgc caggcagcac gctacctgct ggcagttcgc
     7501 cgcacaggat gcgctcggca agatgctgag taacagattc ggagagacta gtctgcacaa
     7561 ttttcattgc atttcctagc taaaaaacgt cacagtgatg agccatataa taagctaaat
     7621 aacagcaaac ttactaattg tgaagcaggt gaactccacc ccctagtcag ttcttatcgt
     7681 tgctggatac cctcaaagca gtgtaatgct atccagaata ggcatgctta aggaagagct
     7741 caagggtaga accccgactt cggcatcgca gaggatactg tagtggtcaa gtaatactgg
     7801 ccacggtttt acagtaaaaa tgatatctgt tctctgactc ttccggcgtc agccctccgt
     7861 tataatggtg aggcctgacg ctattgtaat aattcagaat ataactgctg atttgctgcc
     7921 gggccacgtc tttgcctgtg tagccatcgg ttggcaccca ttctgttttc agactgcgga
     7981 agaagcgttc cattggactg ttatcccagc agtttccccg tcggctgaca ctttgcttta
     8041 tcctgtaacg ccagagaagt tgttgatatt tcagtcctgt atattgactt ccctggtcgc
     8101 tatggaacat gacgtcccgc ggctgaccac gcacctcata cgccatccgc agggcactgc
     8161 ttatcagggc agtatcggca ttcgctgaca ggctccagcc gataaccctg cgggcaaaaa
     8221 gatccatgac gaccgccaga tagcaccagc gatttcctgc ccagatatac gtaatatctc
     8281 cgcaccatac cctatctggc tcgggcacag cgaactggcg ctcaagcaga ttcggcaggc
     8341 aggtatgttc ctgacgagca tttttgtact gatgttttcc gggctgacaa ctgctcaggt
     8401 tcagatattt catcagacgc ccggcacggt aacggctcat cgggacgccg ttttgggtca
     8461 gcatttcagc cagcgtgcgc gcccccgcag agcccctact ttggttccac gcccggcgta
     8521 tttcgctgca caacctgact cgcgccggat taaccgtatc gcgtcgtttt cgccagtacc
     8581 ggtaactact gcggtgtatt tccagagcag aacagaggct gacaactgag tggctgtcac
     8641 tcagtctggc aactatcgtg aaccgttcag cgagtcggac atcaagagcg cggtagcctt
     8701 ttttaatatc gtattgtgtt cctccaggcg gcgaacctgc ttttccagtt cgcggatacg
     8761 ttgctggtct ggagtaatag gtgtggcaaa gggcgcaatc ccctgacgct ctcgcctgag
     8821 ctggcgcacc caactctcaa gcgtggtaga accgacattc atcgcttcac tggcttgtcg
     8881 atatgagtag cccttatcaa caattagctg tgcacattcc agcctgaatt caggggtgaa
     8941 agtacgtttg gttttcttgt tcattaagtc acctgttttg tgttgtggtg aggatatcac
     9001 ctttaatcag gtggccaaat ttactgtgcc actacaaaaa catctctgga ttttagctca
     9061 tatattattg aggcaataaa agagaagttt gaacgagacg acgcaatagt tcaataactt
     9121 gctggatacc taaaggatgt ctgatgtgtg ccaataaaat actttatggg gatccttagt
     9181 gtatctgatg aatttccatc agacatccta gatggaggaa acaggactat agacgctcta
     9241 ccttattctc ctgaacctgt gttgtttcgc ggcgtcttcc tgtaacgact acttctgacg
     9301 ctggcgttaa attattacct gttgagataa cgccgaatga acagaaaaat acgatgatgt
     9361 ctattgctcc atctttatcc atttcccccc ataacacagt cagtgtcagt tcctgtaagg
     9421 ttgagttccg tctcagtaac atgatgtgtt ggaaaatcct tcaccagagc tgctcaaggt
     9481 gttaatccgt gagttagcca ggaggagacg atgatctcac taccatccgg tacccgtatc
     9541 tgcctcattg ccggggtagc tgatatgcgt aaatccttca acggactggg aaaacagggt
     9601 caacatgtgc tgaattataa tcccttcttc ggccacctgt ttatcttccg aggccgacgg
     9661 ggtgacactg ttaaaatact ctgggctgat ggtctgtgtc ttttcaccaa gcgccaggag
     9721 gaaggcaatt tatctggctt gcggtacgtg acggcggggt atcctttacc cgcccgcagc
     9781 tggcaatgct ccttagtaag ctgggctagc gtcagccaaa aacatcacgc cttaacgcgc
     9841 tgacaatgtg gtaaaaatat catagccgga ttataaaaac ggtcatgaat cagaactacc
     9901 tcattcgcat cgctgacgct ggaaaagctg ctgtctgagt actaagtcgt tgaaagcata
     9961 ctcttctttc cctcctgttt agcgcctgat atcccttttc catttctgcc cgacgcatgt
    10021 cattttttta tgcattgttc tttttgtgat ctaaattgta gtacagtaat ataagcttgt
    10081 actacattac gcacacggca aacgcgaacg ttatcacgct ggtactacaa agttgccgcg
    10141 ttatgcatcg atcggggtaa agtagagaag aacatacaga gcacaaggac tctccatgac
    10201 tctcaataaa accgatcgca tcgtcattac gctgggtaaa cagatcgttc acggcaaata
    10261 cgtgccaggc tcgccgcttc cggctgaggc ggagctctgt gaggagtttg caacctcgcg
    10321 caacatcatc cgtgaggtgt tccgttcgct gatggcgaag cggctgattg aaatgaaacg
    10381 ttatcgcggc gcgtttgtgg caccgcgtaa ccagtggaat tacctcgaca ctgacgtact
    10441 gcaatgggtg ctggaaaacg actacgaccc acgacttatc agtgccatga gcgaagtgcg
    10501 aaatctggtg gaaccggcga ttgcccgttg ggcagcagag cgcgcgactt ccagcgatct
    10561 ggcgcagatt gaatcggcgc tgaacgagat gattgccaac aatcaggacc gcgaagcgtt
    10621 taacgaagcg gatattcgct accacgaggc ggtgctgcag tcggtaca
//
LOCUS       DAMZFT010000161        10574 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.161, whole genome shotgun sequence.
ACCESSION   DAMZFT010000161 DAMZFT010000000
VERSION     DAMZFT010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10574)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10574)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10574
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.161"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            162..989
                     /gene="mepH"
                     /locus_tag="PN977_004360"
     CDS             162..989
                     /gene="mepH"
                     /locus_tag="PN977_004360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-endopeptidase MepH"
                     /protein_id="HDI6044315.1"
                     /translation="MARINRISITLCALLFTTLPLTPMAHASKQARESSATTHITKKA
                     DKKKSTATTKKTQKASQKTAKKAASKSTIKSKTASSVKKSSITASKNAKTRSKHAVNK
                     TASANFTEKCTKRKGYKSHCVKVKNAASGTLADAHKAKVQKATKVAMNKLMQQIGKPY
                     RWGGSSPRTGFDCSGLVYYAYKDLVKIRIPRTANEMYHLRDAAPIERSELKNGDLVFF
                     RTQGRGTADHVGVYVGNGKFIQSPRTGQEIQITSLSEDYWQRHYVGARRVMTPKTLR"
     gene            1117..1698
                     /gene="sodB"
                     /locus_tag="PN977_004361"
     CDS             1117..1698
                     /gene="sodB"
                     /locus_tag="PN977_004361"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Fe]"
                     /protein_id="HDI6044316.1"
                     /translation="MSFELPALPYAKDALAPHISAETIEYHYGKHHQTYVTNLNNLIK
                     GTAFEGKSLEEIIRSSEGGVFNNAAQVWNHTFYWNCLAPNAGGEPTGKVAEAIAASFG
                     SFADFKAQFTDAAIKNFGSGWTWLVKNSDGKLAIVSTSNAGTPLTTDATPLLTVDVWE
                     HAYYIDYRNARPGYLEHFWALVNWEFVAKNLAA"
     gene            complement(1844..3013)
                     /locus_tag="PN977_004362"
     CDS             complement(1844..3013)
                     /locus_tag="PN977_004362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDI6044317.1"
                     /translation="MKINYPLLALAIGAFGIGTTEFSPMGLLPVIARGVDVSIPAAGM
                     LISAYAVGVMVGAPLMTLLLSHRARRSALIFLMAIFTLGNVLSAIAPDYMTLMLSRIL
                     TSLNHGAFFGLGSVVAASVVPKHKQASAVATMFMGLTLANIGGVPAATWLGETIGWRM
                     SFLATAGLGVISMVSLFFSLPKGGAGARPEVKKELAVLMRPQVLSALLTTVLGAGAMF
                     TLYTYISPVLQSITHATPVFVTAMLVLIGVGFSIGNYLGGKLADRSVNGTLKGFLLLL
                     MVIMLAIPFLARNEFGAAISMVVWGAATFAVVPPLQMRVMRVASEAPGLSSSVNIGAF
                     NLGNALGAAAGGAVISAGLGYSFVPVMGAIVAGLALLLVFMSARKQPETVCVANS"
     gene            complement(3179..3268)
                     /gene="ynhF"
                     /locus_tag="PN977_004363"
     CDS             complement(3179..3268)
                     /gene="ynhF"
                     /locus_tag="PN977_004363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein YnhF"
                     /protein_id="HDI6044318.1"
                     /translation="MSTDLKFSLVTTIIVLGLIVAVGLTAALH"
     gene            3567..4592
                     /gene="purR"
                     /locus_tag="PN977_004364"
     CDS             3567..4592
                     /gene="purR"
                     /locus_tag="PN977_004364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor PurR"
                     /protein_id="HDI6044319.1"
                     /translation="MATIKDVAKRANVSTTTVSHVINKTRFVAEETRNAVWAAIKELH
                     YSPSAVARSLKVNHTKSIGLLATSSEAAYFAEIIEAVEKNCFQKGYTLILGNAWNNLE
                     KQRAYLSMMAQKRVDGLLVMCSEYPEPLLAMLEEYRHIPMVVMDWGEAKADFTDAVID
                     NAFEGGYMAGRYLIERGHREIGVIPGPLERNTGAGRLAGFMKAMEEAMIKVPESWIVQ
                     GDFEPESGYRAMQQILSQPHRPTAVFCGGDIMAMGALCAADEMGLRVPQDVSLIGYDN
                     VRNARYFTPALTTIHQPKDSLGETAFNMLLDRIVNKREEPQSIEVHPRLIERRSVADG
                     PFRDYRR"
     gene            complement(4589..5521)
                     /locus_tag="PN977_004365"
     CDS             complement(4589..5521)
                     /locus_tag="PN977_004365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDI6044320.1"
                     /translation="MWSEYSLEVVDAVARNGSFSAAAQELHRVPSAVSYTVRQLEEWL
                     AVPLFERRHRDVELTAAGAWFLKEGRSVVKKMQITRQQCQQIANGWRGQLAIAVDNIV
                     RPERTRQMIVDFYRHFDDVELLVFQEVFNGVWDALSDGRVELAIGATRAIPVGGRYAF
                     RDMGMLSWSCVVASHHPLALMDGPFSDDTLRNWPSLVREDTSRTLPKRITWLLDNQKR
                     VVVPDWESSATCISAGLCIGMVPTHFAKPWLNEGKWVALELENPFPDSACCLTWQQND
                     MSPALTWLLEYLGDSETLNKEWLREPEETPATGD"
     gene            5634..6845
                     /locus_tag="PN977_004366"
     CDS             5634..6845
                     /locus_tag="PN977_004366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bcr/CflA family multidrug efflux MFS
                     transporter"
                     /protein_id="HDI6044321.1"
                     /translation="MQPGKRFLVWLAGLSVLGFLATDMYLPAFAAIQADLQTPASAVS
                     ASLSLFLAGFAAAQLLWGPLSDRYGRKPVLFIGLTIFALGSLGMLWVENAATLLVLRF
                     VQAVGVCAAAVIWQALVTDYYPSQKVNRIFATIMPLVGLSPALAPLLGSWLLVHFSWQ
                     AIFATLFAITVVLILPIFWLKPTTKARNNSQDGLTFTDLLRSKTYRGNVLIYAACSAS
                     FFAWLTGSPFILSEMGYSPAVIGLSYVPQTIAFLIGGYGCRAALQKWQGKQLLPWLLV
                     LFAVSVIATWAAGFISHVSLVEILIPFCVMAIANGAIYPIVVAQALRPFPHATGRAAA
                     LQNTLQLGLCFLASLVVSWLISISTPLLTTTSVMLSTVVLVALGYMMQRCEEVGCQNH
                     GNAEVAHSESH"
     gene            7136..8284
                     /gene="cfa"
                     /locus_tag="PN977_004367"
     CDS             7136..8284
                     /gene="cfa"
                     /locus_tag="PN977_004367"
                     /EC_number="2.1.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane fatty acyl phospholipid synthase"
                     /protein_id="HDI6044322.1"
                     /translation="MSSSCIEEVSVPDDNWYRIANELLSRAGIAINGSAPADIRVKNP
                     DFFKRVLQEGSLGLGESYMDGWWECDRLDMFFSKVLRAGLENQLPHHFKDTLRIAGAR
                     LFNLQSKKRAWIVGKEHYDLGNDLFSRMLDPFMQYSCAYWKDADNLESAQQAKLKMIC
                     EKLQLKPGMRVLDIGCGWGGLAHYMASNYDVSVVGVTISAEQQKMAQERCEGLDVTIL
                     LQDYRDLNDQFDRIVSVGMFEHVGPKNYDTYFAVVDRNLKPEGIFLLHTIGSKKTDLN
                     VDPWINKYIFPNGCLPSVRQIAQSSEPHFVMEDWHNFGADYDTTLMAWYERFLAAWPE
                     IADNYSERFKRMFTYYLNACAGAFRARDIQLWQVVFSRGVENGLRVAR"
     gene            complement(8324..8965)
                     /gene="ribE"
                     /locus_tag="PN977_004368"
     CDS             complement(8324..8965)
                     /gene="ribE"
                     /locus_tag="PN977_004368"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HDI6044323.1"
                     /translation="MFTGIVQGTAKLVSIDEKPNFRTHVVELPDHMLDGLETGASVAH
                     NGCCLTVTEINGNHVSFDLMKETLRITNLGDLKVGDWVNVERAAKFSDEIGGHLMSGH
                     IMTTAEVAKILTSENNRQIWFKVQDSQLMKYILYKGFIGIDGISLTVGEVTPTRFCVH
                     LIPETLERTTLGKKKLGARVNIEIDPQTQAVVDTVERVLAARENAMNQPGTEA"
     gene            9180..10553
                     /gene="mdtK"
                     /locus_tag="PN977_004369"
     CDS             9180..10553
                     /gene="mdtK"
                     /locus_tag="PN977_004369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MATE transporter MdtK"
                     /protein_id="HDI6044324.1"
                     /translation="MQKYISEARLLLALAIPVILAQIAQTAMGFVDTVMAGGYSATDM
                     AAVAIGTSIWLPAILFGHGLLLALTPVIAQLNGSGRRERIAHQVRQGFWLAGFVSVLI
                     MLVLWNAGYIIRSMENIDPALADKAVGYLRALLWGAPGYLFFQVARNQCEGLAKTKPG
                     MVMGFIGLLVNIPVNYIFIYGHFGMPELGGVGCGVATAAVYWVMFLAMVSYIKRARSM
                     RDIRNEKGTAKPDPAVMKRLIQLGLPIALALFFEVTLFAVVALLVSPLGIVDVAGHQI
                     ALNFSSLMFVLPMSLAAAVTIRVGYRLGQGSTLDAQTAARTGLMVGVCMATLTAIFTV
                     SLREQIALLYNDNPEVVTLAAHLMLLAAVYQISDSIQVIGSGILRGYKDTRSIFYITF
                     TAYWVLGLPSGYILALTDLVVEPMGPAGFWIGFIIGLTSAAIMMMLRMRFLQRLPSAI
                     ILQRAAR"
BASE COUNT         2593 a         2717 c         2572 g         2692 t
ORIGIN      
        1 ccatttttag ctttcactgc tgcatttttt tgcgctcgcc aacgaaacgt attttttaac
       61 aataaaagct attaactttc tcttcttcta tgcattagaa tcatcaagtt ttgtaaatca
      121 gacgcaggca tgatagacct gccttaacag agggacgctc agtggcgcgg ataaaccgta
      181 tttcgatcac gctctgtgct ttacttttta ccaccctgcc tttaacgcct atggcccatg
      241 cttcaaagca agccagggag agttctgcta ctactcatat caccaaaaaa gcagataaaa
      301 agaaaagcac ggcaaccacc aaaaagaccc agaaagccag ccagaaaaca gcgaaaaaag
      361 ccgccagtaa aagtacgatc aaaagcaaaa ccgcttcttc cgttaaaaaa tcttccatta
      421 ccgcttctaa aaacgccaaa actcgcagca aacacgccgt caataaaacg gcctcagcca
      481 actttaccga aaagtgtacc aaacgtaagg gctataaatc gcattgtgtg aaagtcaaaa
      541 atgccgcgtc aggaaccctt gccgacgcgc acaaagcgaa ggtgcaaaaa gccacgaaag
      601 tggcaatgaa taaactgatg cagcaaattg gtaagccata tcgttggggc ggcagctcac
      661 cgcgtaccgg ttttgattgc agcggcctgg tttattacgc ttataaagat ttggtgaaaa
      721 ttcgtattcc gcgtacggcg aatgaaatgt atcacctgcg agatgcagcg ccaatcgaac
      781 gtagtgaact gaaaaacggc gacctggtct ttttccgtac tcagggacgc ggtacagccg
      841 atcatgtcgg cgtatatgtc ggcaacggca aatttattca gtcaccgcgt acgggtcagg
      901 aaattcaaat cacttctctc agtgaagact actggcagcg ccactatgtt ggcgctcgcc
      961 gggtaatgac cccaaaaaca cttcgctaaa actttaccct gttgttacgg caacagggta
     1021 agttcatctt ttgtctcacc ttttaatttg ctaccctatc catacgcaca ataaggctat
     1081 tgtacgtatg caaattaata ataaaggaga gtagcaatgt cattcgaatt acctgcacta
     1141 ccatatgcta aagatgctct ggcaccgcac atttctgcgg aaaccatcga gtatcactac
     1201 ggcaagcatc atcagactta tgtgactaac ctgaacaacc tgattaaagg taccgcgttt
     1261 gaaggtaaat cactggaaga gattattcgc agctctgaag gtggcgtatt caacaacgca
     1321 gctcaggtct ggaaccatac tttctactgg aactgcctgg caccgaacgc gggtggcgaa
     1381 ccgactggaa aagtcgctga agctatcgcc gcatcttttg gcagctttgc cgatttcaaa
     1441 gctcagttta ctgatgcagc gatcaaaaac tttggttctg gctggacctg gctggtgaaa
     1501 aacagcgatg gcaagctggc tatcgtttca acctctaacg cgggtactcc actgaccacc
     1561 gatgcaactc cgctgctgac cgttgatgtc tgggaacacg cttattacat cgactatcgc
     1621 aatgcacgtc ctggctatct ggagcacttc tgggcgctgg tgaactggga attcgtagcg
     1681 aaaaatctcg ctgcataata actgatggca aatgcagcat tgcctgatgc gcttcgctta
     1741 ccatgccaac acggatgagt cgttaacacc gcatccggca agataaatcg cacgttgcca
     1801 gcaactgtaa cgcagaaggt tatccttctg cgtttttgtt taattagctg ttggcaacgc
     1861 aaactgtttc aggttgtttt ctggctgaca taaacaccag taataatgcc agtcccgcga
     1921 caatcgcccc catcaccggc acaaaactgt atcccagccc agcggaaatt accgcaccac
     1981 cagcagctgc tcccagcgca tttccaagat taaaggcacc aatattgact gatgaagaca
     2041 gacctggcgc ttcactggcg acacgcatca cgcgcatctg taacggcggt acgaccgcaa
     2101 aggttgcagc gccccacacc accatgctaa tagctgcgcc gaactcattg cgggccagga
     2161 acgggattgc cagcataatc accatcagca gcaacaaaaa gcctttcaac gtgccgttaa
     2221 ctgaacgatc tgccagtttg ccgccgagat agttaccgat agagaatccg acaccaatca
     2281 gcaccagcat tgccgtaacg aacaccggtg ttgcgtgggt aatactttgc agtaccggag
     2341 agatataggt gtagagggta aacattgcac cagctcccag taccgtcgtc agcaatgcag
     2401 acagcacctg cggacgcatt aataccgcca gctctttttt cacttcaggt cgcgcccctg
     2461 cgccaccttt aggtaatgag aagaacagac ttaccattga aatcactccc agccccgccg
     2521 ttgccagaaa tgacatccgc cagccgatgg tttcacccaa ccaggtcgcc gccggcacgc
     2581 caccgatatt tgccagggtt aaccccataa acatagtggc aactgcgctg gcctgtttat
     2641 gttttggcac cacgcttgcg gccacaactg aacccaaacc aaaaaatgct ccgtgattca
     2701 ggctggtcaa aatgcgtgaa agcatcagag tcatataatc cggcgcgatg gcggaaagta
     2761 cgttgccgag cgtgaaaatc gccatcagga aaatcaacgc actgcggcga gcacgatgag
     2821 aaagtagaag cgtcatcagc ggcgcgccaa ccattacgcc aactgcatag gcactgatta
     2881 acattccggc agcgggaatc gagacatcca caccgcgcgc aatgacgggc aataagccca
     2941 ttggcgagaa ctccgttgtc ccgataccaa acgcgccaat cgccagcgcc agcaacggat
     3001 agttaatttt catgccttat ctccacctct tcgcgtcatt acgcggtatt cattaaagtg
     3061 gcgaaagcat gacagcaatc acaaaaaaat gaaaataaca aaaagagaaa acacttttgc
     3121 cattttgcta acaaacagga aggagatgcg agggagaacg cgctccctcg agaggaaatc
     3181 agtgcagcgc ggcagtcaaa cccacggcca cgatcaaacc gaggacgata atcgttgtta
     3241 ccagtgaaaa tttaaggtcg gtgctcatca agttttctcc ttttttatta ccacacaaaa
     3301 agtgatatta cgcattttta cacactgtga tgaaaaaatc tcccgtcatt tataatgata
     3361 agtgttttta ccacttcccc ttttcgtcaa gatcggccaa aattccacgc ttacactatt
     3421 tgcgtactgg ccattgaccc cttcctgacg ctccgtgtcg tttttccggc gtaccgcaac
     3481 acttttgttg tgcgtaaggt gtgtaaaggc aaacgtttac cttgcgattt tgcaggagct
     3541 gaagttaggg tctggagtga aatggaatgg caacaataaa agatgtagcg aaacgagcaa
     3601 acgtttccac tacaactgtg tcacacgtga tcaacaaaac acgtttcgtc gctgaagaaa
     3661 cgcgcaacgc cgtgtgggca gcgattaaag aattacacta ctcccctagc gcggtggcgc
     3721 gtagcctgaa ggttaaccac accaagtcta tcggtttgct ggcgaccagc agcgaagcgg
     3781 cctattttgc cgagatcatt gaagcggttg aaaaaaattg cttccagaaa ggttacaccc
     3841 tgattctggg caatgcgtgg aacaatcttg agaaacagcg ggcttatctg tcgatgatgg
     3901 cgcaaaaacg cgtcgatggt ctgctggtga tgtgttctga gtacccagag ccgttgctgg
     3961 cgatgctgga agagtatcgc catatcccaa tggtggtgat ggactggggt gaagcaaaag
     4021 ctgacttcac cgatgcggtc attgataacg cgttcgaagg cggctacatg gccgggcgtt
     4081 atctgataga acgcggtcac cgcgaaatcg gcgttatccc tggcccgctg gaacgtaaca
     4141 ccggcgcagg ccgccttgcc ggttttatga aggcgatgga agaagcgatg atcaaagtgc
     4201 cggaaagctg gattgtacag ggtgactttg aacctgaatc cggttatcgc gccatgcagc
     4261 aaatactgtc gcaaccgcat cgccctactg ccgtcttctg tggtggcgat atcatggcaa
     4321 tgggcgcact ttgtgctgct gatgaaatgg gtctgcgcgt gccgcaggat gtttcgctga
     4381 tcggttatga taacgtgcgc aacgcccgct attttacgcc agcgctgacc acgatccatc
     4441 agccaaaaga ttcgctgggc gaaacggcgt tcaacatgct gttggatcgt atcgtcaata
     4501 aacgcgaaga accgcaatcc attgaagtgc atccgcggtt gattgaacgc cgctccgtgg
     4561 ctgacggccc gttccgcgac tatcgtcgtt aatcacccgt tgcgggagtc tcttccggct
     4621 cccgcagcca ctccttattc agcgtctcac tatcgccgag atactcaagc aaccaggtta
     4681 acgcaggcga catatcattt tgctgccatg tcagacaaca tgccgaatcc gggaaggggt
     4741 tttccagttc tagtgctacc cacttcccct cattaagcca cggtttggcg aaatgtgttg
     4801 gcaccatccc tatgcataat cctgccgaga tacaggttgc cgatgattcc cagtcaggca
     4861 cgacaactct tttttggtta tccagcaacc aggtaatacg tttaggtagc gttcgcgagg
     4921 tgtcttcgcg caccaatgac ggccagttgc gcaacgtatc atcgctgaac gggccatcca
     4981 tcaacgccag cgggtggtga ctggcaacaa cgcaactcca gcttagcatc cccatatccc
     5041 ggaaggcata acgaccacct accggaatcg cgcgtgttgc gccaatcgcc agttccacgc
     5101 gcccgtcgga aagcgcatcc cagacaccgt tgaacacttc ctgaaagaca agaagttcga
     5161 catcatcaaa atggcgataa aaatcaacga tcatctgccg tgtacgttct ggcctgacaa
     5221 tattatccac tgcgatagct aactgaccgc gccagccgtt cgctatctgc tgacattgct
     5281 gccgggtgat ctgcattttt ttgacaacag agcgcccttc tttgagaaac cacgccccgg
     5341 cagcggtcag ttccacatca cggtgacgtc gttcaaacag cggcaccgcc agccactctt
     5401 ccagctgacg cacggtatag ctgaccgcag aaggaacgcg atgcaactcc tgtgccgcag
     5461 cgctaaaact accattacgc gctaccgcat caacaacttc gagtgaatat tctgaccaca
     5521 tagtctgcct gcaaaatttt tgaaaccagt catcaaatat taccgtttca caacactaat
     5581 ttcactccct acactttgcg gcggtgttta attgagagat ttagagaata tacatgcaac
     5641 ctgggaaaag atttttagtc tggctggcag gtttgagcgt actcggtttt ctggcaaccg
     5701 atatgtatct gcctgctttc gccgccatac aggccgacct gcaaacgcct gcgtctgctg
     5761 tcagtgccag ccttagtctg ttccttgccg gatttgccgc agcccagctt ctgtgggggc
     5821 cgctctccga ccgttatggt cgtaaaccgg tattattcat cggcctgaca atttttgcgt
     5881 taggtagtct ggggatgctg tgggtagaaa acgccgctac gctgctggta ttgcgttttg
     5941 tacaggctgt gggtgtctgc gccgcggcgg ttatctggca agcgttagtg acggattatt
     6001 atccttcaca gaaagttaac cgtatttttg cgaccatcat gccgctggtg ggtctatctc
     6061 cggcactggc tcctctgtta ggaagctggc tgctggtcca tttttcctgg caggcgattt
     6121 tcgccaccct gtttgccatt accgtggtgc tgattctgcc tattttctgg ctcaaaccca
     6181 cgacgaaggc ccgtaacaat agtcaggatg gtctgacctt taccgacctg ctacgttcta
     6241 aaacctatcg cggcaacgtg ctgatatatg cggcctgttc agccagtttt tttgcatggc
     6301 tgaccggctc accgttcatc cttagtgaaa tgggttacag cccggcagtt attggtttaa
     6361 gttatgtccc gcaaactatc gcgtttctga ttggtggtta tggctgtcgc gccgcactgc
     6421 agaaatggca aggcaagcag ttattaccgt ggttgctggt actgtttgct gtcagcgtca
     6481 ttgcgacctg ggctgcgggc ttcattagcc atgtgtcgct agtcgaaatc ctgatcccat
     6541 tctgtgtgat ggcgattgcc aacggtgcga tctacccgat tgttgtcgct caggcgctgc
     6601 gtcccttccc acatgcaact ggtcgcgccg cagcgttgca gaacactctg caactgggtc
     6661 tgtgcttcct cgcaagtctg gtagtttcct ggcttatcag tatcagcacg ccattgctca
     6721 ccaccaccag cgtgatgtta tcaacagtag tgctggtcgc tctgggttac atgatgcaac
     6781 gttgtgaaga agttggctgc cagaatcatg gcaatgccga agtcgctcat agcgaatcac
     6841 actgatctat atcgatatac ttatacttag gctgctaaca aaattttgtt gtatgattga
     6901 aattagcggc ctatactaat ttcgagttgt taaagctacg ataaatatta tgtttttacg
     6961 gggacaggat cgttcccgac tcactatgga tggtcatttc ggcaagggtt cctcctttcc
     7021 ctctgttcta cgtcggatta tagactcgcg gttttttctg cgagatttct cacaaagccc
     7081 aaaaagcgtc tacgctgttt taaggttctg atcaccgacc agtgatggag aaactatgag
     7141 ttcatcgtgt atagaagaag tcagtgtacc ggatgacaac tggtaccgta tcgccaacga
     7201 attacttagc cgtgccggta tagccattaa cggttctgcc ccggcggata ttcgtgtcaa
     7261 aaaccccgat ttttttaaac gcgttctgca ggaaggatcg ttggggttag gcgaaagtta
     7321 tatggatggc tggtgggaat gtgaccgact ggatatgttt tttagcaaag tcttacgcgc
     7381 aggtctcgag aaccaactcc cccatcattt caaagacacg ctgcgtattg ccggcgctcg
     7441 tctcttcaat ctgcagagta aaaaacgtgc ctggatagtc ggcaaagagc attacgattt
     7501 gggtaatgac ttgttcagcc gcatgcttga tcccttcatg caatattcct gtgcttactg
     7561 gaaagatgcc gataatctgg aatctgccca gcaggcgaag ctcaaaatga tttgtgaaaa
     7621 attgcagtta aaaccaggga tgcgcgtact ggatattggc tgtggctggg gcggactggc
     7681 gcactacatg gcgtctaatt atgacgtaag cgtggtcggc gtaaccattt ctgccgaaca
     7741 gcaaaaaatg gctcaggaac gctgtgaagg cctggatgtc accattttgc tgcaagatta
     7801 tcgtgacctg aacgaccagt ttgaccgtat tgtttctgtg gggatgttcg aacacgtcgg
     7861 accgaaaaat tacgatacct attttgcggt ggtggatcgt aatttgaaac cggaaggtat
     7921 attcctgctc cataccatcg gttcgaaaaa aaccgatctg aatgttgatc cctggattaa
     7981 taaatatatt tttccgaacg gttgcctgcc ctctgtacgc cagattgctc agtccagcga
     8041 accccacttt gtgatggaag actggcataa cttcggtgct gattacgata ctacgttgat
     8101 ggcgtggtat gaacgtttcc tcgccgcatg gccagaaatt gcggataact acagtgaacg
     8161 ctttaaacgg atgtttacct attatctgaa tgcctgtgca ggtgctttcc gcgcacgtga
     8221 tattcagctc tggcaggtgg tgttctctcg cggagtcgaa aacggccttc gagtcgctcg
     8281 ctagaggcta ttctatcgcc cccgctccgg gggcgatttc agttcaggct tctgtacctg
     8341 gttgattcat ggcattttct cgtgccgcca gcacacgttc taccgtatca accactgcct
     8401 gagtttgtgg atcgatttca atgttgacgc gtgcgccaag ttttttcttc ccaagagtcg
     8461 tgcgttccag tgtttccgga attaaatgga cgcaaaaacg cgttggcgtg acttcgccga
     8521 cggtcaggct aataccgtcg atgccaataa atcctttgta caggatatat ttcatcaact
     8581 gactatcctg gactttaaac cagatctgac gattattttc cgaggttaat attttcgcta
     8641 cttcagcggt ggtcataata tgacctgaca ttaagtgtcc gccaatttca tcgctgaatt
     8701 tcgccgcacg ctcaacgttt acccaatccc ccacttttaa atcgccaaga ttagtaatgc
     8761 gtaacgtttc tttcatcagg tcaaaactga catggttgcc gttaatttcc gtcacggtca
     8821 ggcagcaacc gttatgcgcc acggaagcac cggtttccag accgtccagc atgtggtcgg
     8881 gtaactccac cacatgcgtg cgaaaatttg gtttctcgtc aatcgacacc agttttgcgg
     8941 tgccctgtac aatccccgta aacatactta caactcctga aatcagttaa gacattctgt
     9001 tcagcacaat agcaggtgga aaacgccctt accagtgaag gggtaagaat ggctattttt
     9061 tctctggaga attaataaat cctcgctaca atagactgaa tttcccctgc ttcttctttt
     9121 tgctgcccat tcaggcggct ttttagtctc tcatataact acaaataaaa ggtgttcacg
     9181 tgcagaagta tatcagtgaa gcgcgtctgt tattagcatt agcaatcccg gtgattctcg
     9241 cgcaaatcgc ccaaactgcg atgggttttg tcgataccgt gatggcgggc ggctatagtg
     9301 ccaccgacat ggcggcggtc gctatcggaa cttctatctg gcttccggcg atcctctttg
     9361 gtcacggact gctgctggca ttaacgccgg ttatcgcgca attaaatggc tccggtcgac
     9421 gtgagcgcat tgcgcatcag gtgcgacaag gtttctggct ggcaggtttt gtttccgttc
     9481 tcattatgct ggtgctgtgg aatgcaggct acattatccg ctccatggaa aacatcgatc
     9541 cggctctggc ggacaaagcc gtgggttatc tgcgtgcgtt gctatggggc gcgccgggct
     9601 atcttttctt ccaggttgcc cgtaaccagt gtgaaggtct ggcaaaaacc aagccgggta
     9661 tggtgatggg ctttatcggc ctgctggtga acattccggt gaactatatc tttatttatg
     9721 gtcatttcgg tatgcctgag ctcggtggcg ttggttgtgg cgtggctact gcggcggtgt
     9781 attgggtcat gttccttgcc atggtttctt acattaaacg cgcccgctcg atgcgcgata
     9841 ttcgtaacga aaaaggcacc gcgaagcccg atcctgcggt tatgaaacga ctgattcaac
     9901 tcggtttgcc aattgcgctg gcactgttct ttgaagtaac gctgtttgcc gtcgtggctc
     9961 tgttagtgtc tccgctcggt attgttgatg tcgcaggaca tcagattgcc ctgaacttta
    10021 gttcactgat gttcgtgctt cccatgtcgc tggcggcagc ggtaactatc cgcgtaggtt
    10081 atcgtctggg tcaaggctca acgctggatg cgcaaaccgc tgcgcggacc gggcttatgg
    10141 tgggtgtctg tatggcaacc ctgacggcca tattcacggt ttcactgcga gagcaaatcg
    10201 ccctgttgta caacgacaat cccgaggttg taacgctggc tgcgcatttg atgctgctgg
    10261 cggcggtata tcagatttct gactctatcc aggtgattgg cagtgggatt ttgcgtggtt
    10321 ataaagatac acgttccatt ttctatatca cctttacggc ttactgggtg ctgggcttgc
    10381 caagcggcta tattctggca ctgaccgatc tggtcgttga acctatgggg ccagcaggct
    10441 tctggatagg ctttattatt ggcctgactt cggcagccat tatgatgatg ctgcgtatgc
    10501 ggttcctgca acgtctgccg tcagccatca ttctgcaacg agcagctcgc tagtaaagac
    10561 aaggcgcaac cttc
//
LOCUS       DAMZFT010000162        10433 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.162, whole genome shotgun sequence.
ACCESSION   DAMZFT010000162 DAMZFT010000000
VERSION     DAMZFT010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10433)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10433)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10433
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.162"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(<1..132)
                     /locus_tag="PN977_004370"
     CDS             complement(<1..132)
                     /locus_tag="PN977_004370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000560980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HDI6044325.1"
                     /translation="MIALIQRVTRASVTVEGEVTGEIGAGLLVLLGVEKDDDEQKANR
                     "
     gene            complement(129..1001)
                     /locus_tag="PN977_004371"
     CDS             complement(129..1001)
                     /locus_tag="PN977_004371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor BrkB family protein"
                     /protein_id="HDI6044326.1"
                     /translation="MLKTIQDKARHRTRPLWAWLKLLWQRIDEDNMTTLAGNLAYVSL
                     LSLVPLVAVVFALFAAFPMFSDVSIQLRHFIFANFLPATGDVIQRYIEQFVANSNKMT
                     AVGACGLIVTALLLMYSIDSALNTIWRSKRARPKIYSFAVYWMILTLGPLLAGASLAI
                     SSYLLSLRWASDLNTVIDNVLRIFPLLLSWISFWLLYSIVPTIRVPNRDAIVGAFVAA
                     LLFEAGKKGFALYITMFPSYQLIYGVLAVIPILFVWVYWTWCIVLLGAEITVTLGEYR
                     KLKQAAEQEEDDEP"
     gene            complement(995..1594)
                     /gene="yihX"
                     /locus_tag="PN977_004372"
     CDS             complement(995..1594)
                     /gene="yihX"
                     /locus_tag="PN977_004372"
                     /EC_number="3.1.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphatase"
                     /protein_id="HDI6044327.1"
                     /translation="MLYIFDLGNVIVDIDFNRVLGAWSDLTRVPLATLKKSFHMGEAF
                     HQHERGEISDEAFAEALCHEMALPLSYEQFSHGWQAVFVALRPEVIAIMHKLREQGHR
                     VVVLSNTNRLHTTFWPEEYPEIRDAADHIYLSQDLGMRKPEARIYQHVLQAEGFSPSD
                     TVFFDDNADNIEGANQLGITSILVKDKTTIPDYFAKVLC"
     gene            complement(1693..2478)
                     /locus_tag="PN977_004373"
     CDS             complement(1693..2478)
                     /locus_tag="PN977_004373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418320.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HDI6044328.1"
                     /translation="MSLTELTGNPRHDQLLMLIAERGYMNIDELANLLDVSTQTVRRD
                     IRKLSEQGLITRHHGGAGRASSVVNTAFEQREVSQTEEKKAIAEAVADYIPDGSTIFI
                     TIGTTVEHVARALLNHNHLRIITNSLRVAHILYHNPRFEVMVPGGTLRSHNSGIIGPS
                     AASFVADFRADYLVTSVGAIESDGALMEFDVNEANVVKTMMAHARNILLVADHTKYHA
                     SAAVEIGNVAQVTALFTDELPPAALKSRLQDSQIEIILPQEDA"
     gene            complement(2512..3408)
                     /gene="yihV"
                     /locus_tag="PN977_004374"
     CDS             complement(2512..3408)
                     /gene="yihV"
                     /locus_tag="PN977_004374"
                     /EC_number="2.7.1.184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418319.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfofructose kinase"
                     /protein_id="HDI6044329.1"
                     /translation="MIRVACVGITVMDRIYYVEGLPTESGKYVARNYTEVGGGPAATA
                     AVAAARLGAQVDFIGRVGDDDTGNSLLAELESWGVNTRYTKRYNQAKSSQSAIMVDAK
                     GERIIINYPSPDLLPDAEWLEEIDFSQWDVVLADVRWHDGAKKAFTLARQAGVMTVLD
                     GDITPQDISELVALSDHAAFSEPGLARLTGVKEMASALKQAQTLTNGHVYVTQGSAGC
                     DWLENGGRQHQPAFKVDVVDTTGAGDVFHGALAVALATGGDLAESVRFASGVAALKCT
                     RPGGRAGIPDCDQTRSFLSLFV"
     gene            3576..4472
                     /gene="yihU"
                     /locus_tag="PN977_004375"
     CDS             3576..4472
                     /gene="yihU"
                     /locus_tag="PN977_004375"
                     /EC_number="1.1.1.373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfolactaldehyde 3-reductase"
                     /protein_id="HDI6044330.1"
                     /translation="MAAIAFIGLGQMGSPMASNLLQQGHQLRVFDVNAEAVRHLVDKG
                     ATPAANPAQAAKDAEFIITMLPNGDLVRSVLFGENGVCESLSTDALVIDMSTIHPLQT
                     DKLIADMQARGFSMMDVPVGRTSANAITGTLLLLAGGTAEQVERATPILMAMGSELIN
                     AGGPGMGIRVKLINNYMSIALNALSAEAAVLCEALNLPFDVAVKVMSGTAAGKGHFTT
                     SWPNKVLSGDLSPAFMIDLAHKDLGIALDVANQLHVPMPLGAASREVYSQARAAGRGR
                     QDWSAILEQVRVSAGMTAKVKM"
     gene            4496..5374
                     /gene="yihT"
                     /locus_tag="PN977_004376"
     CDS             4496..5374
                     /gene="yihT"
                     /locus_tag="PN977_004376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfofructosephosphate aldolase"
                     /protein_id="HDI6044331.1"
                     /translation="MNKYTINDITRASGGFAMLAVDQREAMRMMFAAAGAPAPVADSV
                     LTDFKVNAAKTLSPYASAILVDQQFCYRQVVEQNAIAKSCAMIVAADEFIPGNGIPVD
                     SVVIDRKINPQQIKQDGGKALKLLVLWRSDEDAQQRLDMVKEFNELCHSHGLVSIIEP
                     VVRPPRRGDKFDREQAIIDAAKELGDSGADLYKVEMPLYGKGPQQELLSASQRLNDHI
                     NMPWVILSSGVDEKLFPRAVRVAMTAGASGFLAGRAVWASVVGLPDNELMLRDVCAPK
                     LQQLGDIVDEMMAKRR"
     gene            5390..6631
                     /gene="yihS"
                     /locus_tag="PN977_004377"
     CDS             5390..6631
                     /gene="yihS"
                     /locus_tag="PN977_004377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfoquinovose isomerase"
                     /protein_id="HDI6044332.1"
                     /translation="MKWFNTLSHNRWLEQETDRIFDFGKNSVVPTGFGWLGNKGQIKE
                     EMGTHLWITARMLHVYSVAAAMGRPGAYSLVDHGIKAMNGALRDKKYGGWYACVNDEG
                     VVDASKQGYQHFFALLGAASAVTTGHPEARKLLDYTIEIIEKYFWSEEEQMCLESWDE
                     AFSKTEEYRGGNANMHAVEAFLIVYDVTHDKKWLDRAIRVASVIIHDVARNNHYRVNE
                     HFDTQWNPLPDYNKDNPAHRFRAFGGTPGHWIEWGRLMLHIHAALEARGEQPPAWLLE
                     DAKGLFNATVRDAWAPDGADGIVYTVDWEGKPVVRERVRWPIVEAMGTAYALYTVTGD
                     RQYETWYQTWWEYCIKYLMDYENGSWWQELDADNKVTTKVWDGKQDIYHLLHCLVIPR
                     IPLAPGLAPAVAAGLLDVNAK"
     gene            6750..7649
                     /locus_tag="PN977_004378"
     CDS             6750..7649
                     /locus_tag="PN977_004378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose-1-epimerase"
                     /protein_id="HDI6044333.1"
                     /translation="MQTTNMHCSGQTVSLTAGDYHATIVTVGAGLAELTFQGCHLVIP
                     HKPEEMPLAHLGKVLIPWPNRIANGCYRYQGQEYQLPINEHGSKAAIHGLLAWRDWQI
                     SEQTTTSVTLTAFLPPSYGYPFMLASQVVYSLNARTGLSVEIASQNIGTVAAPYGVGI
                     HPYLTCNLTPVDDYVFQLPAEQVYAVEHANPTTLHHVDELDLNFTQAKKIAATKIDHT
                     FKTANDLWEMTITHPQQVLSVSLCSDQPWVQVYSGEKLQRQGLAVEPMSCPPNAFNSG
                     IDLLLLEPGKTHRLFFNIYGQRK"
     gene            7847..9883
                     /gene="yihQ"
                     /locus_tag="PN977_004379"
     CDS             7847..9883
                     /gene="yihQ"
                     /locus_tag="PN977_004379"
                     /EC_number="3.2.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfoquinovosidase"
                     /protein_id="HDI6044334.1"
                     /translation="MDTPRPQLLDFQFHQNNDSFTLRFQDRLILIHSKDNPCLSIGSG
                     IADIDMFRGNFSIKDKLQEKIALTDAIVSQSPDGWLIHFSRGSDISATLRISADDQGR
                     LLLELQNDNHHHNRIWLRLAAQPEDHIYGCGEQFSYFDLRGKPFPLWTSEQGVGRNKQ
                     TYVTWQADCKENAGGDYYWTFFPQPTFVSTQKYYCHVDNSCYMNFDFSAPEYHELALW
                     EEKATLRFECADTYISLLEKLTALLGRQPELPDWIYDGVTLGIQGGTEVCQKKLDTMR
                     NAGVKVNGIWAQDWSGIRMTSFGKRVMWNWKWNSENYPQLDSRIKQWNKEGVQFLAYI
                     NPYVASDKDLCAEAASHGYLAKDASGGDYLVEFGEFYGGVVDLTNPEAYAWFKEVIKK
                     NMIELGCGGWMADFGEYLPTDTYLHNGVSAEIMHNAWPALWAKCNYEALEETGKLGEL
                     LFFMRAGSTGSQKYSTMMWAGDQNVDWSLDDGLASVVPAALSLAMTGHGLHHSDIGGY
                     TTLFEMKRSKELLLRWCDFSAFTPMMRTHEGNRPGDNWQFDSDAETIAHFARMTTVFT
                     TLKPYLKEAVALNAKSGLPVMRPLFLHYEDDAQTYTLKYQYLLGRDILVAPVHEEGRS
                     DWTLYLPEDNWVHAWTGEAFRGGEVTVNAPIGKPPVFYRAGSEWAALFATLKSI"
     gene            9929..>10433
                     /locus_tag="PN977_004380"
     CDS             9929..>10433
                     /locus_tag="PN977_004380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDI6044335.1"
                     /translation="MSHITTEDPATLRLPFKEKLSYGIGDLASNILLDIGTLYLLKFY
                     TDVLGLPGTYGGIIFLISKFFTAFTDMGTGIMLDSRRKIGPKGKFRPFILYASFPVTL
                     LAIANFIGTPFDVTGKTVMATILFMLYGLFFSMMNCSYGAMVPAITKNPNERASLAAW
                     RQGGATLG"
BASE COUNT         2577 a         2760 c         2662 g         2434 t
ORIGIN      
        1 acggtttgct ttctgttcgt cgtcatcctt ttcgacaccc aataacacca aaagtcccgc
       61 gccaatttcg cccgtcactt ctccctccac ggtgacgctg gcacgggtta cgcgttgaat
      121 taatgcaatc atggttcgtc gtcttcttct tgttcagctg cttgttttag tttgcggtat
      181 tccccgagag tgacagtaat ttccgcacca agcaagacga tacaccatgt ccagtagacc
      241 cagacaaaga gaatggggat caccgccagc acaccgtaaa tgagctgata tgaggggaac
      301 atggtgatat aaagcgcgaa acctttcttt cctgcttcga acaggagtgc ggcgacaaac
      361 gcgccgacaa tcgcgtcgcg attaggtacg cggatggtag gaacaatgct gtacagcaac
      421 cagaaggaga tccacgacaa cagcagcgga aaaatacgca gcacgttgtc gatgacagta
      481 ttgagatcgc tcgcccagcg cagggagagc aaataggaac tgatcgccag actggcccct
      541 gccagcagcg gccccagcgt taaaatcatc cagtacacgg caaacgagta aatttttggt
      601 cgcgctcgtt tactgcgcca gatggtattc aacgcgctat cgatggagta catcaacaat
      661 aacgccgtga cgatcagccc gcacgcccca acggcggtca tcttgttgga attggcaaca
      721 aattgttcga tataccgctg aataacatcg ccagtagcag gcagaaagtt ggcaaaaata
      781 aagtgacgca actgaatgct gacgtcggaa aacatgggaa aagcggcgaa aagcgcaaaa
      841 acaacggcaa ccagcggcac taatgagagc aacgacacat aggcaaggtt acctgccagg
      901 gttgtcatgt tgtcctcatc aatgcgttgc cagagtagtt ttagccaggc ccatagtgga
      961 cgggtacggt gcctggcttt gtcctgaatg gtttttagca taacaccttc gcgaaatagt
     1021 ccgggatggt ggttttatct ttcaccagaa tactggtaat gcctagctga ttcgctcctt
     1081 ctatattatc ggcgttatcg tcgaaaaaga ccgtatcgct gggtgaaaaa ccttctgcct
     1141 gcaaaacatg ctgataaatt cgtgcttcag gtttgcgcat ccccagatct tgcgacagat
     1201 agatatggtc agcagcatca cggatttccg ggtattcctc cggccagaag gtagtatgca
     1261 ggcggttagt attggaaagc accaccacgc gatgcccctg ctcacgcagt ttatgcatga
     1321 tggcgatcac ttccgggcgc agcgcgacaa acaccgcctg ccagccgtga gagaactgct
     1381 cgtagcttag cggtagagcc atctcatgac acagcgcctc tgcgaacgct tcgtcgctaa
     1441 tttccccacg ctcatgctga tgaaacgcct cgcccatgtg gaaactcttc ttaagcgtag
     1501 ccagtggaac acgcgttaaa tcgctccagg ctcccagcac acggttaaag tcgatatcga
     1561 caatcacatt acctaaatca aagatataga gcatttttgc ctcctttcgc gccacaagaa
     1621 attaactgta gcgggaaagg atgggtttgg ctatgtggtc aggtatgaag ccaggtcaac
     1681 ggtcacaaaa ttctacgcgt cttcctgggg aaggatgatt tcaatttggc tgtcttgtaa
     1741 gcgtgatttt agcgcagcgg gcggcagctc gtcggtaaag agcgcagtga cctgtgccac
     1801 attaccaatt tcaaccgccg ccgaagcatg atacttagtg tgatcggcga ccagcagaat
     1861 atttctcgcg tgcgccatca tcgttttcac cacgttagct tcgttaacat caaactccat
     1921 caacgcgcca tcgctctcaa ttgcgcctac acttgttacc agataatcgg cgcgaaaatc
     1981 ggccacaaag gacgccgctg aagggccaat gatcccactg ttatgggaac gcagcgttcc
     2041 gccaggcacc atcacttcaa agcgcgggtt gtggtaaaga atatgcgcca cacgcaggct
     2101 gttggtgatt atccgcaaat ggttatggtt aagtaacgcc cgggcaacat gctcaacggt
     2161 cgtaccaatg gtgataaata ttgtcgatcc atcaggaata tagtctgcca ccgcttcggc
     2221 aatcgctttt ttctcttcag tttgcgaaac ctcacgctgc tcgaacgccg tattaacgac
     2281 gctggaagcc cgacccgcgc caccgtgatg gcgcgtaatc agtccttgct cgcttaattt
     2341 acgaatatcc cggcggaccg tctgcgtgga gacatccagc agatttgcca gctcatcaat
     2401 attcatatac ccacgctcgg cgatcagcat gaggagttgg tcgtgccgcg ggttaccggt
     2461 tagttcggta aggctcatga aaattcctcg aaaaaccatc accctggcat tttatacaaa
     2521 aagtgacaaa aaagatcggg tttgatcaca gtcagggatc ccagctcgtc caccgggacg
     2581 tgtgcatttt aacgccgcta caccgctggc gaagcggact gactccgcta aatccccacc
     2641 tgtggccagc gccaccgcca aagcgccgtg aaaaacatca cccgctcctg tggtatctac
     2701 cacatcaact ttgaaggccg gttgatgttg acgcccgcca ttttccagcc agtcgcagcc
     2761 tgcgctgccc tgggtcacat agacatgacc atttgtgagc gtttgtgcct gttttagggc
     2821 actcgccatc tctttcacgc ccgttaagcg cgccagaccc ggttctgaaa aagctgcatg
     2881 atcgcttaac gccaccagtt cgctgatatc ctgcggcgta atatcaccgt ccagcacggt
     2941 cattacgccc gcctgccgcg ccagagtgaa ggcttttttc gcgccgtcgt gccagcgtac
     3001 atctgccagc accacgtccc actgagagaa atcaatttcc tccaaccact ctgcatctgg
     3061 cagtaggtct gggctgggat agttaatgat gattcgctca cctttggcat ccaccatgat
     3121 ggcggattgc gaagatttcg cctggttata ccgtttggtg taacgggtgt taacgcccca
     3181 ggattccagc tctgccagca ggctgttacc cgtgtcgtca tcaccgacgc gaccaataaa
     3241 atcgacctgc gcccccagcc ttgccgccgc aaccgctgcc gtcgccgctg gcccgccacc
     3301 aacttccgta taatttctcg ccacgtattt accgctctcc gtcggtaatc cttccacgta
     3361 ataaatacga tccatcacgg taatacctac acaagcaaca cgaatcatgg tcattcctta
     3421 agcattttgg gatgcgttca ttttaatttc accaattgtt taaaaagtga cggctgtcaa
     3481 ttttttgacg ataaattaca aatacgatca aaaacagaca agcaaagcat gtcataaatg
     3541 acaaaaagtg acatcactgt attcaggaga gggttatggc agcaatcgcg tttatcggtt
     3601 taggacaaat gggttcgcca atggcgagca atttattgca gcaagggcat caacttcgcg
     3661 tctttgatgt gaacgccgaa gctgtgcggc atctggtaga caaaggtgcg actcccgccg
     3721 ccaacccggc gcaggccgct aaagatgccg aatttatcat taccatgttg ccgaatggcg
     3781 atctggtgcg cagcgtgttg ttcggtgaaa acggcgtttg cgaaagctta tctaccgatg
     3841 cgctggtcat cgatatgtcg accatccatc cgctgcaaac cgataaattg attgccgata
     3901 tgcaggccag aggcttcagc atgatggatg ttccggtagg ccgtacttct gcaaatgcca
     3961 ttaccggtac tctgttactg ctggctggcg gcaccgctga acaagttgaa cgcgccacac
     4021 cgatcctgat ggcgatgggc agcgagttga tcaacgcggg cggtccgggc atggggatcc
     4081 gcgttaagct tatcaacaac tacatgagca tcgcgctcaa tgcgttgtcg gcagaagccg
     4141 ccgttttgtg cgaagccctc aatcttccct tcgatgttgc cgtcaaggta atgagcggta
     4201 ctgccgccgg taaaggccac ttcaccactt cctggccgaa caaagtcctc agcggggatc
     4261 tttctcccgc cttcatgatc gatcttgccc ataaggatct tggcatcgcc cttgatgtcg
     4321 ccaaccagtt gcatgtgccg atgccactgg gggccgcctc acgggaggtt tatagccagg
     4381 cgcgcgcagc gggtcgcggt cgccaggact ggtccgccat tctggagcag gtccgtgtca
     4441 gtgccgggat gactgccaaa gtaaaaatgt aacgactgga taaaggaata aatgaatgaa
     4501 taagtacacc atcaacgaca ttacgcgtgc atcgggcggt tttgccatgc tggcggtcga
     4561 tcagcgcgaa gccatgcgca tgatgtttgc cgcggctggc gcacccgctc cagtagccga
     4621 tagcgtttta actgatttca aagttaacgc cgcgaagact ctttcacctt atgcatcggc
     4681 gattctggta gatcaacaat tctgctaccg ccaggtggtt gagcaaaacg caattgccaa
     4741 aagctgcgcc atgatcgtcg ctgccgatga atttattcca ggcaacggta ttccggtgga
     4801 tagcgtagtc attgaccgca aaatcaaccc gcaacaaatc aaacaagacg gtggcaaagc
     4861 cttaaaactg ctggtgctgt ggcgtagtga tgaagatgcg cagcaacgcc tggatatggt
     4921 gaaagagttc aatgaactgt gccactcaca cggtctggtg agcatcattg agccagtcgt
     4981 tcgtccgccg cgtcgtggcg ataaattcga tcgcgaacaa gcaatcatcg atgcagccaa
     5041 agagctgggt gatagcggtg cagacctcta caaagttgaa atgccgcttt acggcaaagg
     5101 tccgcaacaa gagcttctca gtgcttcaca acgtttgaat gaccatatca atatgccgtg
     5161 ggtgatcctc tcttccggtg tcgacgaaaa actgttcccg cgtgccgtac gcgtggcaat
     5221 gacggcgggc gcatcgggat tccttgcagg ccgtgcagtc tgggcatcgg tcgtcggttt
     5281 accagacaac gagttgatgc tgcgtgacgt ttgcgcaccg aaattacaac aacttggcga
     5341 tatcgtcgac gaaatgatgg ctaaacgccg ttaagatggg gatacaaaaa tgaaatggtt
     5401 taacacctta agtcacaacc gttggctgga acaggaaacc gatcgcatct ttgattttgg
     5461 taaaaattcc gtggtgccga ctggttttgg ctggttaggc aataaagggc aaatcaaaga
     5521 agagatgggc acccatctgt ggatcaccgc ccgtatgtta cacgtttatt ccgttgctgc
     5581 ggcgatgggt cgaccaggcg cttactcgtt ggttgatcac ggtatcaaag ccatgaacgg
     5641 cgcactgcgc gataaaaaat atggcggctg gtatgcctgt gtgaatgacg agggcgtggt
     5701 ggatgcctcc aaacagggct atcaacattt ctttgccctg ctgggtgccg ccagtgccgt
     5761 cacaacgggt cacccggaag cgcgcaagct gctcgattac accattgaaa ttatcgagaa
     5821 atacttctgg agcgaagaag agcagatgtg cctggaatcc tgggacgaag ccttcagtaa
     5881 aaccgaagag taccgcggcg gcaacgccaa catgcatgcg gtagaagcct tcttgattgt
     5941 ttatgacgta acgcatgaca aaaaatggct ggatcgtgcg attcgcgtag cttccgtcat
     6001 tatccacgac gtcgccagaa ataatcatta tcgcgttaac gagcacttcg atacccagtg
     6061 gaatccgctg ccggattaca acaaagataa cccggcgcac cgcttccgcg cattcggagg
     6121 cacgccgggc cactggatcg agtggggtcg tttaatgctg cacatccatg ccgccctgga
     6181 agcccgcggc gaacagccgc cagcctggct gttagaagat gccaaaggtc tgttcaacgc
     6241 caccgtgcgc gatgcctggg cgcccgatgg tgcggacggg attgtttata ccgttgactg
     6301 ggaaggaaaa ccggtcgttc gcgaacgtgt gcgttggcct atcgtcgagg cgatgggtac
     6361 ggcttacgcg ctctacaccg tcaccggcga tcgccagtac gaaacctggt atcagacatg
     6421 gtgggagtac tgcattaagt acctgatgga ctatgaaaat ggttcctggt ggcaggagct
     6481 ggatgcggac aataaggtca ccaccaaagt ctgggacggc aaacaggata tttatcacct
     6541 gctgcattgc ctggtgatcc cgcgtatccc gttagcccct ggtctggctc cggcagttgc
     6601 ggcggggctg ctggacgtga atgccaaata actgactgaa aaccctaacc ctctccccaa
     6661 aggggagagg ggaccgcccg gcgcagtttt ttggtttgac atcagcctca tgggaggctg
     6721 tataccgttt tttaattaag gtacctcgta tgcaaaccac caatatgcat tgcagtggac
     6781 agaccgtttc actcaccgct ggggattatc acgcaactat cgtgacggtt ggcgcaggtc
     6841 tggctgaatt aacttttcag ggctgccatt tagttattcc gcataaaccc gaagagatgc
     6901 cgctggcaca cctgggcaaa gtccttattc cctggcctaa ccggattgcc aacggctgtt
     6961 atcgctatca ggggcaggaa tatcagttac ccattaatga acatggttca aaagccgcta
     7021 ttcacgggct gcttgcctgg cgtgactggc aaataagcga acaaaccacc accagcgtca
     7081 cattaacggc ctttttgccg cccagttacg gctatccgtt tatgctggcc tcgcaagttg
     7141 tttattcgct caacgcacgc acgggccttt ccgttgagat cgcaagccag aatatcggca
     7201 ctgtcgcagc accttatggt gtgggtattc atccttattt aacctgcaac ttaacgcctg
     7261 ttgatgacta tgtattccag cttccagcag aacaggttta tgccgtcgag cacgcgaatc
     7321 cgacaacgct tcaccacgtt gatgagctgg atttaaattt cacgcaggcg aaaaaaatag
     7381 ccgcgacaaa aatcgaccac acctttaaaa ccgctaatga tctgtgggaa atgaccatta
     7441 cccatccaca acaggttctt tcggtaagcc tttgttccga tcaaccctgg gtgcaggttt
     7501 atagcggaga aaaattacag cgccagggcc tggcagttga acctatgagt tgcccgccta
     7561 acgcctttaa ttcaggtatc gacttattat tactggaacc aggaaaaacg caccgcctgt
     7621 tttttaatat ttacggacag agaaaataac ttgtattgat tagcagtcaa aatcattgaa
     7681 gaacatttca ctcgtaaagg caacgttatg tatcttcaat ttattaagtt aaaaaaagct
     7741 cagcttctgt aaagtttata tatatcagtt ttatgagaat cattttacat aagcctgtaa
     7801 taacgggaag gaaacaatct cgtcctcaac tctatggaga ttatttatgg atacgccacg
     7861 cccacagcta ttagattttc aatttcatca gaataacgac agttttactc tacgttttca
     7921 agaccgcctt attttaatcc atagcaaaga taatccttgc ttatcgattg gctcaggtat
     7981 agcggatatc gatatgttcc gcggtaattt cagcattaaa gataaattgc aggagaaaat
     8041 tgcgcttacc gacgccatcg tcagccagtc accggatggt tggttaatcc atttcagccg
     8101 tggttctgac attagcgcca cgctgcgtat ctctgccgac gatcagggcc gtttattgct
     8161 ggaactacaa aacgacaacc atcaccacaa ccgcatctgg ctgcgtcttg ccgcacaacc
     8221 agaggaccat atctacggct gcggcgaaca attctcctac tttgatctgc gcggcaaacc
     8281 gttcccgcta tggaccagtg aacaaggcgt tggtcgcaac aaacaaacct atgtcacctg
     8341 gcaggccgac tgcaaagaga acgcgggcgg cgactattac tggactttct tcccgcagcc
     8401 tacgtttgtc agcacgcaga agtattactg ccatgttgat aacagttgct atatgaactt
     8461 cgactttagt gccccggaat accatgaact ggcgctgtgg gaagagaaag caacactgcg
     8521 ttttgaatgt gctgacacat acatctccct gttagaaaaa ttaaccgccc tgctgggacg
     8581 ccagccagaa ctgcccgact ggatttatga cggggtaacg ctcggcattc agggcgggac
     8641 tgaagtgtgc cagaagaaac tggacaccat gcgtaacgcg ggcgtgaagg tcaacggcat
     8701 ctgggcgcag gactggtccg ggatccgtat gacctcgttt ggtaaacgtg tgatgtggaa
     8761 ctggaagtgg aacagcgaaa actatccgca actggattcg cgtattaaac agtggaacaa
     8821 agaaggcgtg cagttcctcg cctacatcaa cccgtatgtt gccagcgata aggacctctg
     8881 cgccgaggcg gcttcacacg gctatctggc aaaagatgcc tctggcggcg actatctggt
     8941 ggagtttggc gagttctatg gcggcgttgt cgatctcacc aatccagaag cctacgcctg
     9001 gttcaaggaa gtgatcaaaa agaacatgat tgaacttggc tgcggcggct ggatggctga
     9061 cttcggcgag tatctgccca ccgacacgta cctgcataac ggcgtcagcg cggaaattat
     9121 gcataacgcc tggcctgcgc tgtgggcgaa atgtaactac gaagcccttg aagaaacagg
     9181 caagctcggc gagctcctct tctttatgcg cgccggttct accggtagcc agaaatactc
     9241 caccatgatg tgggcgggcg accagaacgt tgactggagt ctggacgatg gactggcgtc
     9301 agtcgtcccg gcggcgctgt ctctggcaat gaccggacat ggcctgcatc acagtgatat
     9361 tggcggttac accaccctgt ttgagatgaa gcgcagcaaa gagctgctgc tgcgctggtg
     9421 cgatttcagc gccttcacgc cgatgatgcg cacccacgaa ggtaaccgtc ctggcgacaa
     9481 ctggcagttt gatagcgacg cggaaactat cgcccatttt gcccgtatga ccaccgtctt
     9541 caccaccctg aaaccttacc tgaaagaggc cgtcgcgctg aatgcgaagt ccggcctgcc
     9601 ggttatgcgc ccgctgttcc tgcattacga agacgacgcg cagacctaca ccctgaaata
     9661 tcagtacctg ttaggccgcg acattctggt cgctccggtg catgaagaag gccgtagcga
     9721 ctggacgctc tatctgccgg aggataactg ggtccatgcc tggacgggtg aagcgttccg
     9781 gggcggggaa gttaccgtta atgcgcccat cggtaaaccg ccggtctttt atcgcgccgg
     9841 tagcgaatgg gcggcactgt tcgccacgtt aaaaagtatc taatcatgtc cgcccgtggg
     9901 gaaactcacg ggcaaaggga gaacaacgat gagtcacatc acaacggaag atccagcaac
     9961 tttacgcctg ccctttaaag agaaactctc ttacggtatt ggcgacctgg cctctaacat
    10021 cctgctggat attggtacgc tttatctttt gaagttttat accgacgttc tggggctgcc
    10081 tggcacctat ggtggcatta tctttttgat ttcaaaattc tttactgcgt ttaccgatat
    10141 gggaacaggc atcatgctgg attcccgacg caagatcggc ccaaaaggca agttccgtcc
    10201 ttttattctg tatgcgtcat tcccggtcac cctgctggcg atcgccaact ttatcggtac
    10261 gccgttcgat gtcaccggta aaacggtgat ggccaccatc ctgtttatgc tttacggact
    10321 gtttttcagc atgatgaact gctcgtatgg cgcgatggtc cccgctatta ctaaaaaccc
    10381 caacgaacgt gcatcgctgg cagcatggcg tcagggtggc gctacattag gcc
//
LOCUS       DAMZFT010000163        10384 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.163, whole genome shotgun sequence.
ACCESSION   DAMZFT010000163 DAMZFT010000000
VERSION     DAMZFT010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10384)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10384)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10384
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.163"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(<1..134)
                     /gene="rfaH"
                     /locus_tag="PN977_004381"
     CDS             complement(<1..134)
                     /gene="rfaH"
                     /locus_tag="PN977_004381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription/translation regulatory transformer
                     protein RfaH"
                     /protein_id="HDI6044336.1"
                     /translation="MQSWYLLYCKRGQLQRAQEHLERQAVNCLAPMISLEKIVRGKRT
                     A"
     gene            301..1794
                     /gene="ubiD"
                     /locus_tag="PN977_004382"
     CDS             301..1794
                     /gene="ubiD"
                     /locus_tag="PN977_004382"
                     /EC_number="4.1.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-polyprenylbenzoate decarboxylase"
                     /protein_id="HDI6044337.1"
                     /translation="MDAMKYNDLRDFLTLLEQQGELKRITLPVDPHLEITEIADRTLR
                     AGGPALLFENPKGYSMPVLCNLFGTPKRVAMGMGQEDVSALREVGKLLAFLKEPEPPK
                     GFRDLFDKLPQFKQVLNMPTKRLRGAPCQQKIVSGDDVDLNRIPIMTCWPEDAAPLIT
                     WGLTVTRGPHKERQNLGIYRQQLIGKNKLIMRWLSHRGGALDYQEWCVAHPGERFPVS
                     VALGADPATILGAVTPVPDTLSEYAFAGLLRGTKTEVVKCISNDLEVPASAEIVLEGY
                     IEQGETAPEGPYGDHTGYYNEVDSFPVFTVTHITQREDAIYHSTYTGRPPDEPAVLGV
                     ALNEVFVPILQKQFPEIVDFYLPPEGCSYRLAVVTIKKQYAGHAKRVMMGVWSFLRQF
                     MYTKFVIVCDDDVNARDWNDVIWAITTRMDPARDTVLVENTPIDYLDFASPVSGLGSK
                     MGLDATNKWPGETQREWGRPIKKDPDVVAHIDAIWDELAIFNNGKSA"
     gene            1840..2541
                     /gene="fre"
                     /locus_tag="PN977_004383"
     CDS             1840..2541
                     /gene="fre"
                     /locus_tag="PN977_004383"
                     /EC_number="1.5.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-flavin reductase"
                     /protein_id="HDI6044338.1"
                     /translation="MTTLSCKVTSVEAITDTVYRVRIVPDAAFSFRAGQYLMVVMDER
                     DKRPFSMASTPDEKGFIELHIGASEINLYAKAVMDRILKDHQIVVDIPHGEAWLRDDE
                     ERPMILIAGGTGFSYARSILLTALARNPNRDITIYWGGREEQHLYDLSELEALSLKHP
                     GLQVVPVVEQPEAGWRGRTGTVLTAVLQDHGTLAEHDIYIAGRFEMAKIARDLFCSER
                     NAREDRLFGDAFAFI"
     gene            complement(2732..3895)
                     /gene="fadA"
                     /locus_tag="PN977_004384"
     CDS             complement(2732..3895)
                     /gene="fadA"
                     /locus_tag="PN977_004384"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadA"
                     /protein_id="HDI6044339.1"
                     /translation="MEQVVIVDAIRTPMGRSKGGAFRHVRAEDLSAHLMRSLLARNPA
                     LEAAALDDIYWGCVQQTLEQGFNIARNAALLAEVPHSVPAVTVNRLCGSSMQALHDAA
                     RMIMTGDAQACLVGGVEHMGHVPMSHGVDFHPGLSRNVAKAAGMMGLTAEMLARMHGI
                     SREMQDAFAARSHARAWAATQSGAFKNEIIPTGGHDADGVLKQFNYDEVIRPETTVEA
                     LATLRPAFDPVSGTVTAGTSSALSDGAAAMLVMSESRARELGLKPRARVRSMAVVGCD
                     PSIMGYGPVPASKLALKKAGLSASDIGVFEMNEAFAAQILPCIKDLGLMEQIDEKINL
                     NGGAIALGHPLGCSGARISTTLLNLMERKDAQFGLATMCIGLGQGIATVFERI"
     gene            complement(3905..6094)
                     /gene="fadB"
                     /locus_tag="PN977_004385"
     CDS             complement(3905..6094)
                     /gene="fadB"
                     /locus_tag="PN977_004385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadB"
                     /protein_id="HDI6044340.1"
                     /translation="MLYKGDTLYLDWLEDGIAELVFDAPGSVNKLDTATVASLGEAIG
                     VLEQQSDLKGLLLRSNKAAFIVGADITEFLSLFLVPEEQLSQWLHFANSVFNRLEDLP
                     VPTIAAVNGYALGGGCECVLATDYRLATPDLRIGLPETKLGIMPGFGGSVRMPRMLGA
                     DSALEIIAAGKDVGADQALKIGLVDGVVKAEKLIEGAMAILRQAINGDLDWKAKRQPK
                     LEPLKLSEIEATMSFTIAKGMVAQTAGKHYPAPITAVKTIEAAARFGREEALNLENKS
                     FVPLAHTNEARALVGIFLNDQYVKGKAKKLTKDVETPKQAAVLGAGIMGGGIAYQSAW
                     KGVPVVMKDINDKSLTLGMTEAAKLLNKQLERGKIDGLKLAGVISTIHPTLDYAGFDR
                     VDVVVEAVVENPKVKKAVLAETEQKVRPDTVLASNTSTIPISELANALERPENFCGMH
                     FFNPVHRMPLVEIIRGEKSSDETIAKVVAWASKMGKTPIVVNDCPGFFVNRVLFPYFA
                     GFSQLLRDGADFRKIDKVMEKQFGWPMGPAYLLDVVGIDTAHHAQAVMAAGFPQRMQK
                     DYRDAIDALFDANRFGQKNGLGFWRYKEDSKGKPKKEEDAVVDDLLAEVSQPKRDFSE
                     EEIIARMMIPMVNEVVRCLEEGIIATPAEADMALVYGLGFPPFHGGAFRWLDTLGSAK
                     YLDMAQQYQHLGPLYEVPEGLRNKARHNEPYYPPVEPARPVGDLKTA"
     gene            6284..7615
                     /gene="pepQ"
                     /locus_tag="PN977_004386"
     CDS             6284..7615
                     /gene="pepQ"
                     /locus_tag="PN977_004386"
                     /EC_number="3.4.13.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro dipeptidase"
                     /protein_id="HDI6044341.1"
                     /translation="MESLASLYKNHIATLQERTRDALARFKLDALLIHSGELFNVFLD
                     DHPYPFKVNPQFKAWVPVTQVPNCWLLVDGVNKPKLWFYLPVDYWHNVEPLPNSFWTE
                     DVEVIALPKADGIGSLLPAARGNIGYIGPVPERALQLGIEASNINPKGVIDYLHYYRS
                     FKTEYELACMREAQKMAVNGHRAAEEAFRSGMSEFDINIAYLTATGHRDTDVPYSNIV
                     ALNEHAAVLHYTKLDHQAPEEMRSFLLDAGAEYNGYAADLTRTWSAKSDNDYAQLVKD
                     VNDEQLALIATMKAGVSYVDYHIQFHQRIAKLLRKHQIITDMSEEAMVENDLTGPFMP
                     HGIGHPLGLQVHDVAGFMQDDSGTHLAAPAKYPYLRCTRILQPGMVLTIEPGIYFIES
                     LLAPWREGQFSKHFNWQKIEALKPFGGIRIEDNVVIHENNVENMTRDLKLA"
     gene            7615..8229
                     /locus_tag="PN977_004387"
     CDS             7615..8229
                     /locus_tag="PN977_004387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMPACT family protein"
                     /protein_id="HDI6044342.1"
                     /translation="MESWIIPAAPVTVVEEIKKSRFITMLAHTDGVEAAKAFVESVRA
                     EHPDARHHCVAWVAGAPDDSQQLGFSDDGEPAGTAGKPMLAQLMGSGVGEITAVVVRY
                     YGGILLGTSGLVKAYGGGVNQALRQLTTQRKTPLTEYTLQCEYSQLTGIEALLGQCDG
                     KIINSDYQAFVLLRVALPAAKVAEFSAKLADFSRGSLQLLAIEE"
     gene            8268..9719
                     /gene="trkH"
                     /locus_tag="PN977_004388"
     CDS             8268..9719
                     /gene="trkH"
                     /locus_tag="PN977_004388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkH"
                     /protein_id="HDI6044343.1"
                     /translation="MHFRAITRIVGLLVILFSGTMIIPGLVALIYRDGAGRAFTQTFF
                     VALAIGSMLWWPNRKEKGELKSREGFLIVVLFWTVLGSVGALPFIFSESPNLTITDAF
                     FESFSGLTTTGATTLVGLDSLPHAILFYRQMLQWFGGMGIIVLAVAILPILGVGGMQL
                     YRAEMPGPLKDNKMRPRIAETAKTLWLIYVLLTVACALALWFAGMDAFDAIGHSFATI
                     AIGGFSTHDASIGYFDSPTINTIIAIFLLISGCNYGLHFSLLSGRSLKVYWRDPEFRM
                     FIGVQFSLVVICTLVLWFHNVYSSALMTINQAFFQVVSMATTAGFTTDSIARWPLFLP
                     VLLLCSAFIGGCAGSTGGGLKVIRILLLFKQGNRELKRLVHPNAVYSIKLGNRALPER
                     ILEAVWGFFSAYALVFIVSMLAIIATGVDDFSAFASVVATLNNLGPGLGVVADNFTSM
                     NPVAKWILIANMLFGRLEVFTLLVLFTPTFWRE"
     gene            9731..10276
                     /gene="hemG"
                     /locus_tag="PN977_004389"
     CDS             9731..10276
                     /gene="hemG"
                     /locus_tag="PN977_004389"
                     /EC_number="1.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone-dependent protoporphyrinogen IX
                     dehydrogenase"
                     /protein_id="HDI6044344.1"
                     /translation="MKTLILFSTRDGQTREIASYLASELKELGIQADVANVHRIEEPQ
                     WENYDRVVIGASIRYGHYHSAFQEFVKKHATRLNSMPSAFYSVNLVARKPEKRTPQTN
                     SYARKFLMSSQWRPDRCAVIAGALRYPRYRWYDRFMIKLIMKMSGGETDTRKEVVYTD
                     WEQVANFAREIAHLTDKPTLK"
BASE COUNT         2327 a         2722 c         2856 g         2479 t
ORIGIN      
        1 gcagtacgtt tgccacgcac gattttttcc agggagatca tcggtgccag gcaattcaca
       61 gcctgtcttt cgaggtgttc ctgggcacgt tgaagttgcc cgcgcttgca gtacagtaaa
      121 taccaggatt gcataatgac tcttatccgt ttaatcgggg cgcaaggata gcaaaagctt
      181 tacgctaagt taattatatt ccccggtttg cgttataccg tcagagttca cgctaattta
      241 acaaatttac agcatcgcaa agatgaacgc cgtataatgg gcgcagatta agaggctaca
      301 atggacgcca tgaaatataa cgatttacgc gacttcttga cgctgcttga acagcagggt
      361 gagctaaaac gtatcacgct cccggtggat ccgcatctgg aaatcactga aattgctgac
      421 cgcactttgc gtgccggtgg gcctgcgctg ttgttcgaaa accctaaagg ctactcaatg
      481 ccggtgctgt gcaacctgtt cggtacgcca aagcgcgtgg cgatgggcat ggggcaggaa
      541 gatgtttcgg cgctgcgtga agttggtaaa ttattggcgt ttctgaaaga gccggagccg
      601 ccaaaaggtt tccgcgacct gtttgataaa ctgccgcagt ttaagcaagt attgaacatg
      661 ccgacaaagc ggctgcgtgg tgcgccttgc caacaaaaaa tcgtctctgg cgatgacgtc
      721 gatctcaatc gcattcccat tatgacctgc tggccggaag atgccgcgcc gctgattacc
      781 tgggggctga ccgtgacgcg cggcccgcat aaagagcggc agaatctggg catttatcgc
      841 cagcagctga ttggtaaaaa caaactgatt atgcgctggc tgtcgcatcg cggcggcgcg
      901 ctggattatc aggagtggtg tgtggcgcat ccgggcgaac gtttcccggt ttctgtggcg
      961 ctgggtgccg atcccgccac gattctcggt gcagtcaccc ccgttccgga tacgctttca
     1021 gagtatgcgt ttgccggatt gctacgcggc accaaaaccg aagtagtaaa gtgtatttcc
     1081 aatgatctcg aagtgcccgc cagtgcggag attgtgctgg aagggtatat cgaacaaggc
     1141 gaaactgcgc cggaagggcc gtatggcgac cacaccggtt actataacga agtcgatagt
     1201 ttcccggtat ttaccgtgac gcatattacc cagcgtgaag atgcgattta tcattccacc
     1261 tataccgggc gtccgccgga tgaacctgcg gtgctgggtg tcgcactgaa cgaagtgttt
     1321 gtgccgattc tgcaaaaaca gttcccggaa attgtcgatt tttacctgcc gccggaaggc
     1381 tgctcttatc gcctggcggt agtgacgatc aaaaaacagt acgccggaca cgcgaagcgc
     1441 gtcatgatgg gcgtctggtc gttcttacgc cagtttatgt acactaaatt tgtgatcgtt
     1501 tgcgatgatg acgtcaacgc acgcgactgg aacgatgtga tttgggcgat taccacccgt
     1561 atggacccgg cgcgggacac ggtgttggtc gaaaatacgc ctattgatta tctggatttt
     1621 gcctcgcctg tgtccgggct gggttcaaaa atggggctgg atgccacgaa taaatggcca
     1681 ggggaaaccc agcgtgaatg gggacgtccc atcaaaaaag atccggatgt tgtcgcgcat
     1741 attgacgcca tctgggatga actggctatt tttaacaacg gtaaaagcgc ctgatgcgcg
     1801 tttgttttgc cctatttatc gatccgacag agaaagcgca tgacaacctt aagctgtaaa
     1861 gtgacctcgg tagaagctat cacggatacc gtataccgtg tccgaatcgt accagacgcg
     1921 gccttttctt ttcgtgctgg tcagtatttg atggtagtga tggatgagcg tgacaaacgt
     1981 ccgttctcaa tggcttcgac gccagatgaa aaagggttca tcgagctgca tattggcgct
     2041 tctgaaatca acctttacgc gaaagcagtc atggaccgca ttcttaaaga tcatcaaatc
     2101 gtggtcgata ttccgcacgg tgaggcatgg ctgcgcgatg atgaagaacg tccgatgatt
     2161 ttgattgcgg gtggcaccgg gttctcttat gcgcgctcga ttttgctgac ggcgctggcg
     2221 cgtaacccaa accgtgatat caccatttac tggggcgggc gtgaagagca gcatctttat
     2281 gatctctccg agcttgaggc gctttcgttg aagcatcctg gtctgcaagt ggtgccggtg
     2341 gttgaacaac cggaagcggg ctggcgtggg cgtactggca ccgtgttaac ggcggtattg
     2401 caggatcatg gtacgctggc agagcatgat atctatattg ccggacgttt tgagatggcg
     2461 aaaattgccc gtgacctgtt ttgcagcgag cgtaatgcgc gggaagatcg cctgtttggc
     2521 gatgcgtttg catttatctg agatataaaa aacccgcccc tgacaggcgg gaagaacggc
     2581 aactaaaact ttatacagtg gcatttagat ctatgtcgca tccggcaatc ggtgcacgat
     2641 gcctgatgcg acgctggcgc gtcttatcag gcctatattg gtgcaggatc ggtagaccgg
     2701 ataaggcgtt cacgccgcat ccggcaagtg gttaaatccg ctcaaacacc gtcgcaatac
     2761 cctgacccag accgatacac atcgtcgcca gaccaaactg ggcatctttg cgctccatca
     2821 gattcagcag cgtggtgctg atacgcgcac cggaacaacc cagcggatga cccagcgcga
     2881 tcgcgccacc gttgaggttg atcttctcgt caatctgctc cattagcccg agatctttaa
     2941 tacatggcag gatctgcgcg gcaaaggctt cgttcatctc gaatacgccg atatcgctgg
     3001 cagaaagccc cgcttttttc agcgccagct tcgaggccgg aaccgggccg taacccataa
     3061 tcgatgggtc acaaccaacg accgccatcg aacgcacgcg ggcgcgcggc ttaagaccta
     3121 attctcgggc gcggctttca ctcatcacca gcatggcagc tgcgccatcg gaaagcgcag
     3181 aagatgtacc tgccgtcacc gtaccgctga cgggatcaaa cgccgggcgc agcgtggcga
     3241 gggcttccac ggtggtttcc gggcgaatca cttcgtcgta attaaactgc ttcaggacgc
     3301 cgtcggcatc gtgaccaccg gtcgggataa tttcattttt aaatgcaccc gactgcgtgg
     3361 cggcccaggc gcgggcgtgt gaccgcgcgg caaaggcatc ctgcatttca cggctgatac
     3421 cgtgcatacg cgccagcatt tctgccgtta agcccatcat gcccgccgct ttggcgacat
     3481 tgcggctcag gccggggtga aaatcgacgc cgtgactcat cggcacatgg cccatatgct
     3541 ccacgccgcc aaccagacat gcctgcgcat cgccggtcat gatcattcgc gctgcgtcat
     3601 gtaatgcttg catggatgaa ccacacaggc gattgacggt caccgccggg acagagtgtg
     3661 gtacttctgc caacagcgcc gcgttacggg cgatattaaa accttgctcc agcgtctgct
     3721 gtacacagcc ccagtaaata tcgtcgaggg ccgccgcttc cagcgccggg ttacgcgcca
     3781 gcaggctacg cattaaatga gcggagagat cttccgcacg cacgtgacga aaagcaccgc
     3841 ccttcgaacg gcccatcggg gtgcgaattg catcgacaat gacaacctgt tccattgtga
     3901 ctccttaagc cgttttcagg tcgccaaccg gacgggctgg ctcaaccggg ggatagtacg
     3961 gttcgttatg acgcgcttta ttacgcagac cttccggcac ttcatacagc gggccgaggt
     4021 gctgatattg ttgtgccata tcgaggtatt ttgcgctacc gagggtgtcc agccagcgga
     4081 acgcgccgcc gtggaacgga gggaacccca gtccgtagac cagcgccata tccgcttctg
     4141 ctggagtggc gataatgcct tcttccagac agcgcaccac ttcgttgacc atcgggatca
     4201 tcatgcgggc gataatctct tcttcgctga aatcgcgctt cggctggctc acttctgcca
     4261 gcaggtcgtc aacgacggca tcttcttctt tcttcggctt gcctttactg tcttctttat
     4321 aacgccagaa accgaggccg ttcttctgac caaaacggtt ggcatcaaac agcgcatcga
     4381 tggcatcgcg gtaatctttc tgcatccgct gcgggaagcc tgctgccatc accgcctgag
     4441 cgtgatgcgc ggtatcaatc cccacaacgt ccagcagata tgccggcccc atcggccagc
     4501 caaactgttt ttccatcact ttgtcgatct tgcggaaatc cgcgccgtcg cgcagcaact
     4561 ggctgaaacc ggcgaaatag gggaacagta cacggttgac gaagaagccg gggcagtcgt
     4621 taaccacaat cggcgttttg cccatcttgc tcgcccaggc gacgactttc gcgatggttt
     4681 cgtcggagct tttctcgccg cgaataattt ctactaacgg cattcggtgg accgggttaa
     4741 agaagtgcat cccgcagaag ttttccggac gttccagcgc gttggccagt tcgctgatag
     4801 gaatggttga agtgttagac gccagtacgg tatccgggcg tactttttgt tcggtttctg
     4861 ccagtacggc ttttttcact ttcgggtttt caacaaccgc ttctaccaca acatccacgc
     4921 ggtcaaaccc ggcgtagtcg agcgttgggt gaattgtgga gatcacgcca gccagtttca
     4981 gaccatcgat cttgccgcgc tcaagctgct tgttcagcag tttcgcggct tcggtcatgc
     5041 cgagggttaa cgacttgtcg ttgatatctt tcatgacaac cggtacgcct ttccacgcag
     5101 actggtaagc gatgccgcca cccataatgc ctgcgcccag cacagcggcc tgtttcgggg
     5161 tttcaacgtc tttggtgagt ttcttcgctt tgccttttac atattgatcg ttaaggaaaa
     5221 tgccgaccag tgcgcgagct tcgttggtat gcgccagcgg gacaaaactt ttgttttcca
     5281 ggtttagggc ttcttcacga ccaaaacggg ccgcagcttc aatggttttt actgcggtga
     5341 tgggggccgg ataatgtttc cccgccgttt gtgcgaccat ccctttagcg atggtgaagc
     5401 tcatggtggc ttcaatctcg ctcagtttta gcggttccag cttcggctga cgtttcgctt
     5461 tccagtcgag gtcgccgtta atggcctggc gtaaaatcgc catcgcccct tcaatcagtt
     5521 tttctgcttt gactacacca tccaccagac cgattttcag cgcctgatcc gcgccgacat
     5581 ctttaccggc ggcaatgatt tccagcgcac tgtcagcgcc cagcatacgt ggcatacgta
     5641 cagaaccgcc aaagcccggc atgatgccca gtttggtttc cggcagaccg atgcgcagat
     5701 ccggcgtcgc cagacgataa tcggtcgcca gcacgcattc gcagccaccg cccagcgcat
     5761 agccgttgac ggcagcaatg gtcggcaccg gcagatcttc caggcgatta aacacgctat
     5821 tggcaaagtg cagccactga cttaactgtt cttcaggaac gaggaacagg gacaaaaatt
     5881 cggtgatatc agcaccgacg ataaaggctg ctttgttcga acgcagcagc agccctttta
     5941 gatctgattg ctgttccagc acgccgatgg cctcgccgag gctggcgacg gtggcagtgt
     6001 cgagtttatt aactgaacct ggggcatcaa ataccagttc ggcaatgcca tcttccagcc
     6061 agtcaaggta cagggtgtcg cctttgtaaa gcatgtcagt ctcctgaatc cgcaaggtga
     6121 tctggtcgta ccagatgcat cgaagtgtgt attttgtgtt aaaaatatgc aaataaaaga
     6181 ttaaagaaat gccgatctga tcacgctcgg cagaaatcac gctctggatg aacgatgtgc
     6241 taagatgcgg agacttaagg tcaaaaaaac agaagggtaa aaaatggaat cactggcctc
     6301 gctctataaa aatcatatag ctaccttaca ggaacggact cgcgatgcgc tggcgcgctt
     6361 caagctggat gcgttactta ttcactccgg cgaactgttc aacgtttttc tcgacgatca
     6421 tccctatccg tttaaagtga acccgcaatt caaagcgtgg gtgccggtaa ctcaggtgcc
     6481 aaactgctgg ctgctggtgg atggcgtgaa caagccgaaa ctgtggtttt atctgccggt
     6541 tgattactgg cacaacgtcg aaccgctgcc gaactccttc tggactgaag atgtggaagt
     6601 gatcgcgctg ccgaaagccg atggcattgg tagcctgctg cctgctgcgc gcggcaatat
     6661 cggttatatc ggtccggtgc cggaacgtgc gctgcaactg ggtattgagg ccagcaacat
     6721 caatccgaaa ggggtgatcg actacctgca ttactaccgc tccttcaaaa ccgagtacga
     6781 actggcctgt atgcgtgaag cgcagaaaat ggcggtcaac ggtcatcgcg cggcagaaga
     6841 agcgttccgt tctggcatga gcgagtttga tatcaatatt gcctatctga ccgcgaccgg
     6901 tcatcgtgat accgacgtac cttacagcaa cattgtggcg cttaacgaac acgctgcggt
     6961 gctgcattac accaaattgg accaccaggc accggaagag atgcgcagct tcctgctgga
     7021 tgccggggcc gaatataacg gctatgcggc tgacctgacc cgtacctggt cggcaaaaag
     7081 cgacaacgac tacgcacagc tggtgaaaga cgtaaatgat gaacaacttg cgctgatcgc
     7141 caccatgaaa gctggcgtca gctatgtgga ttaccacatc cagttccatc agcgcatcgc
     7201 caaattgctg cgtaaacatc aaatcatcac cgatatgagt gaagaagcga tggtcgaaaa
     7261 cgatctcacc ggaccgttta tgccgcacgg tatcggtcat ccgctgggcc tgcaggtgca
     7321 tgacgtagct ggctttatgc aagatgatag cggtacgcac ctcgcggcac cggcaaaata
     7381 tccgtacctg cgctgcaccc gtattctcca gccaggcatg gtgttaacca tcgaaccggg
     7441 tatctacttc atcgaatcgc tgctggcgcc gtggcgtgaa gggcagttca gcaaacactt
     7501 caactggcag aaaattgaag cactgaaacc gttcggcggc attcgtatcg aagacaacgt
     7561 ggtgatccac gaaaacaacg tggaaaacat gacccgggat ctgaaactgg cgtgatggaa
     7621 agctggataa ttcctgcggc accggtcacg gttgttgaag agatcaaaaa gagccgcttt
     7681 ataacgatgt tggcgcatac cgatggcgtt gaggcggcga aagcgtttgt tgaatcggtg
     7741 cgggcagaac accccgatgc ccgccaccat tgcgtggcgt gggtggcggg tgcgccggat
     7801 gattcacaac agttgggttt ctctgacgac ggggaaccgg cgggaacggc aggtaaaccg
     7861 atgctcgccc aactgatggg cagcggcgtc ggggaaatta ccgccgtggt ggtgcgctac
     7921 tacggcggca tattgcttgg caccagtggg ttagtgaaag cgtatggcgg cggcgtgaat
     7981 caggcgctgc gccagctaac gacccaacgc aagacgccat taaccgaata tactttgcaa
     8041 tgtgaatata gtcagttaac cggcattgaa gcgttgctgg ggcagtgtga cggcaaaatt
     8101 atcaacagtg attatcaggc attcgttctg ctgcgggtgg cgcttccggc ggcgaaagtg
     8161 gctgaatttt ccgcaaagct ggcggatttt agccgtggtt cattgcaatt gttagcgatt
     8221 gaagaataat ccccacttcg ttttgcagaa ctaaggaagc ggcagagatg cattttcgcg
     8281 ccattacccg aatcgttgga ctactggtca tcttattttc agggaccatg attatccctg
     8341 ggctggtagc actcatctac cgggatggag cgggccgcgc ttttacccag accttttttg
     8401 tcgccctcgc cattggctct atgctgtggt ggccgaaccg caaagagaag ggcgaactga
     8461 aatcccgtga ggggtttctg atagtggtgc tgttctggac cgtgctgggt agtgtcggtg
     8521 cgctcccttt tatcttctcg gaaagcccga acctcacgat taccgatgcg ttttttgaat
     8581 ctttctctgg cctgaccacc acgggagcca ctacgctggt ggggctggat tcgctccctc
     8641 atgccatcct cttttatcgc cagatgctgc aatggtttgg cgggatgggg atcatcgtgt
     8701 tggcggttgc gatactgcct atcctcggcg tgggtgggat gcagctctat cgcgcagaaa
     8761 tgcccggccc gctgaaagat aacaaaatgc gcccgcgaat tgcggaaacg gcgaaaaccc
     8821 tgtggttgat ttatgtcttg ctgaccgtcg cctgtgcgct ggcgttgtgg tttgctggaa
     8881 tggatgcctt tgatgccatc ggccatagct ttgcgactat cgctattggc ggcttctcga
     8941 cacatgatgc cagtatcggt tatttcgaca gcccgactat taacactatc attgctatct
     9001 tcctgctgat ctctggctgt aactacggtc tgcacttttc actgttaagt gggcgtagtc
     9061 tgaaggttta ttggcgcgat ccggaatttc gcatgtttat cggcgtacag ttttcactgg
     9121 tggttatttg tacactcgta ctgtggtttc ataatgtcta cagttcggcg ctgatgacaa
     9181 ttaaccaggc gtttttccag gtggtgtcga tggcgacaac cgccgggttt acaactgaca
     9241 gcattgcccg ctggccgctc tttttgccgg tactgctctt atgttcagct tttatcggcg
     9301 gttgtgccgg gtcaacgggc ggtggcctga aagtgatccg catcctgctg ctgtttaagc
     9361 aggggaaccg tgagctgaaa cgactggtgc atccgaacgc cgtctatagc attaagctgg
     9421 ggaatcgcgc actgccggaa cgtatcctcg aagccgtgtg gggatttttc tccgcctatg
     9481 cattggtgtt tattgtcagt atgctggcga ttatcgccac gggcgtggat gacttttctg
     9541 cctttgcctc ggttgttgcg acattgaata acctggggcc agggcttggt gtggttgctg
     9601 ataactttac cagtatgaac ccggtggcta aatggatcct gattgccaac atgctgtttg
     9661 gtcgtctcga ggtctttaca ttgctggtgc tctttacccc gactttctgg cgtgaatgat
     9721 ggagtaatac gtgaaaacat taattctttt ctcaacaagg gacggacaaa cgcgcgagat
     9781 tgcctcctac ctggcttcgg aactgaaaga actggggatc caggcggatg tcgccaatgt
     9841 gcaccgcatt gaagaaccac agtgggaaaa ctacgaccgt gtggtcattg gtgcttctat
     9901 tcgctatggt cactaccact ctgcgttcca ggaatttgtc aaaaagcatg cgacgcgact
     9961 gaattcgatg ccgagcgcct tttactccgt aaacctggtg gcgcgtaaac ctgagaagcg
    10021 tactccacag accaacagct acgcgcggaa gttcctgatg agttcacaat ggcgtcccga
    10081 tcgctgcgcg gtcattgccg gggcgctgcg ttacccacgt tatcgctggt acgaccgctt
    10141 tatgatcaaa ctgattatga agatgtcagg cggtgaaacg gatacgcgca aagaagttgt
    10201 ctataccgat tgggagcagg tggcgaattt cgcccgagaa atcgcccatt taaccgacaa
    10261 accgacgctg aaataagcat aaagaataaa aaatgcgcgg tcggaaaatt attttaaatt
    10321 tcctcttgtc aggccggaat aactccctat aatgcgccac cactgacacg gaacaacggc
    10381 aaac
//
LOCUS       DAMZFT010000164        10372 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.164, whole genome shotgun sequence.
ACCESSION   DAMZFT010000164 DAMZFT010000000
VERSION     DAMZFT010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10372)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10372)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10372
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.164"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(<1..819)
                     /locus_tag="PN977_004390"
     CDS             complement(<1..819)
                     /locus_tag="PN977_004390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyclic terpene utilization AtuA family protein"
                     /protein_id="HDI6044345.1"
                     /translation="MARSFKVLSPTAILGYGFPEESFRKAMAESPDLIAVDAGSSDPG
                     PHYLGAGKPFTDRAGVKRDLRYMITAGVQNNIPVVIGTAGGSGAAPHLEWCREIIHEI
                     AREEQLAFSMALIPADVDKAIVHQALDNGKITALDFVPPLTHDAIDESTYIVAQMGIE
                     PFQRALKEGAQVVLGGRAYDPACFAALPIMQGFDEGLALHCGKILECAAIAATPGSGS
                     DCAMGIIDDNGFTLKTFNPKRKFTETSAAAHTLYEKSDPYFLPGPGGVLNLKGCT"
     gene            complement(823..2064)
                     /locus_tag="PN977_004391"
     CDS             complement(823..2064)
                     /locus_tag="PN977_004391"
                     /EC_number="4.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylaspartate ammonia-lyase"
                     /protein_id="HDI6044346.1"
                     /translation="MKIKQALFTAGYSSFYFDDQQAIKNGAGHDGFFYTGEPVTQGFN
                     AVRQAGECVSVQLILENGAVAVGDCAAVQYSGAGGRDPLFLAEHFIPFLNDHIKPLLV
                     GRDVDAFLPNARFFDKLRIDGNLLHTAVRYGLSQALLDATALATGRLKTEVVCDEWQL
                     PRVAESIPLFGQSGDDRYIAVDKMILKGIDVLPHALINNVEEKLGFKGEKLREYVRWL
                     SDRILSKRTSARYHPTLHIDVYGTIGLIFDMDPLRCAQYIASLEKEAQGLPLYIEGPV
                     DAGNKPDQIRLLTAITKELTRLGSGVKIVADEWCNTYQDIVDFTDAASCHMVQIKTPD
                     LGSIHNIVDAVLYCNSHSMEAYQGGTCNETDVSARTCVHVALAARPMRMLVKPGMGFD
                     EGLDIVFNEMNRTIALLQAKD"
     gene            complement(2064..3509)
                     /locus_tag="PN977_004392"
     CDS             complement(2064..3509)
                     /locus_tag="PN977_004392"
                     /EC_number="5.4.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylaspartate mutase subunit E"
                     /protein_id="HDI6044347.1"
                     /translation="MELRNKKLTHDEFMTERQQVLKTWETGKDVENFEDGVKYQQTIP
                     EHKRFSLALLKADKEGKTLSQPRAGVALMDEHIELLKTLQEECDLLPSTIDAYTRLNR
                     YEEAAVGIKKSIEAGTSKLNGLPVVNHGVAACRRLTETLQKPLQIRHGTPDARLLAEI
                     SMASGFTSYEGGGISYNIPYAKRVTLEKSIRDWQYCDRLMGMYEEHGIRINREPFGPL
                     TGTLIPPFISHSIAIIEGLLALEQGVKSITVGYGQVGSLTQDVAAIQSLRELAHEYFQ
                     SYGYTDYELSTVFHQWMGGFPEDESKAFAIISWGAAVAGMSGATKVITKSPHEAWGIP
                     TAAANIQGLKASRQMLNMVNEQKFPPCPAVELEIELIKSEVRAVLNKVFELGNGDIAR
                     GTVLAFEAGVLDVPFAPAACNAGKILPVRDNTGAIRVLEAGAVPLPKDILDLHHDYVA
                     ERARFEGRQPTFQMVVDDINAVSHSKLIGRP"
     gene            complement(3528..4916)
                     /locus_tag="PN977_004393"
     CDS             complement(3528..4916)
                     /locus_tag="PN977_004393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate mutase L"
                     /protein_id="HDI6044348.1"
                     /translation="MQIVSVDIGSTWTKAALFAREGDALTLVNHVLTPTTTHHLAKGF
                     FSSLNLVLNVDNALPLFNNGEVALKYSSSAKGGLAVAAMGLVPSITLETAKVTAHSAG
                     AKIAQYYSYKLNRRDIQALEETQPDILLFTGGTDGGEESYGLNNARVLAESKLDCAII
                     YAGNRDIQDEVQEILGHKDLTIVDNVLPDLDHPNPLAARQAICDIFLKRIVKGKGLDV
                     VVDKTGEEPMPTPWTVFELVKAISNVDHSWKEFMLIDMGGATTDVYSACANTLSPDTV
                     LHGVPEPFVKRTVEGDLGMRVSAMVVGESAKELVNVNFAQQPAQLDAFYHYLRHLVAH
                     PDYLPANAEEKYFDTVLAGLCVGYATERHAGTKKEVCTCVGNVDLQMGRDLTTVRKVV
                     GTGGWLSRASQFDMHHWLKYRELNDDGKRILLPTEFEYYRDSRGLLPLLANVARVDPL
                     AAARTSIQCLTL"
     gene            complement(4916..5365)
                     /locus_tag="PN977_004394"
     CDS             complement(4916..5365)
                     /locus_tag="PN977_004394"
                     /EC_number="5.4.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylaspartate mutase subunit S"
                     /protein_id="HDI6044349.1"
                     /translation="MKKATLVIGVIGADCHAVGNKVLDRVFSNHGFRVINLGVMVSQD
                     EYIDAAIETGADAIVVSSIYGHGDIDCLGMRERCIERGLGDILLYVGGNLVVGKHDFA
                     DVETKFKEMGFDRVFAPSHDLEDVCQLMAHDINQRHDVDTRILEEAI"
     gene            complement(5912..6334)
                     /locus_tag="PN977_004395"
     CDS             complement(5912..6334)
                     /locus_tag="PN977_004395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6044350.1"
                     /translation="MIPSQISFNTLPVNATYASETTVDTQKFSDILYSAGCSLKDLAY
                     LSRCLASIHPTIAKNIYETENLSDQKLLHIDCRSTNEIKINIFFGQQREGLIEINSDT
                     VIFSILSSLIDSAISKFQHQIPVNGSVNRELLYEDYTD"
     gene            complement(6415..7359)
                     /locus_tag="PN977_004396"
     CDS             complement(6415..7359)
                     /locus_tag="PN977_004396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6044351.1"
                     /translation="MSPDNIGSAVNRFSGWPSNDPLKTDNESMTSPVAVQVIKMIAAI
                     LDPYRIVVGIDTLYTTQRETFTSESQQLIESALQTLHVINLNNLETNLDEDGHYLAFG
                     YNVDTPDRITVSAYNEKKPGCYGQCLLIQDDETDLFNLIKVKIFEARHNYVAQLMRDP
                     EFMFKFSYSAQQNRLEPHLLVPTYFPLKTNEPVTQEDVLLLYRFFKMDGDFNILTSDE
                     YMSIMTPLMKCERSIHDHNRYVTGKDTLLLDYPPSGNQIYFHIFPDESATLVLYFSNN
                     DIECFVFERDIPSQYRFFKMFTNLALVIDQLTDANQIL"
     gene            8165..9733
                     /gene="cydA"
                     /locus_tag="PN977_004397"
     CDS             8165..9733
                     /gene="cydA"
                     /locus_tag="PN977_004397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415261.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HDI6044352.1"
                     /translation="MLDIVELSRLQFALTAMYHFLFVPLTLGMAFLLAIMETVYVLSG
                     KQIYKDMTKFWGKLFGINFALGVATGLTMEFQFGTNWSYYSHYVGDIFGAPLAIEGLM
                     AFFLESTFVGLFFFGWDRLGKVQHMCVTWLVALGSNLSALWILVANGWMQNPIASDFN
                     FETMRMEMVSFSELVLNPVAQVKFVHTVASGYVTGAMFILGISAWYMLKGRDFAFAKR
                     SFAIAASFGMAAVLSVIVLGDESGYEMGDVQKTKLAAIEAEWETQPAPAAFTLFGIPD
                     QEEETNKFAIQIPYALGIIATRSVDTPVIGLKELMVQHEERIRNGMKAYSLLEQLRSG
                     STDQAVRDQFNSMKKDLGYGLLLKRYTPNVADATEAQIQQATKDSIPRVAPLYFAFRI
                     MVACGFLLLAIIALSFWSVIRNRIGEKKWLLRAALYGIPLPWIAVEAGWFVAEYGRQP
                     WAIGEVLPTAVANSSLTAGDLIFSMVLICGLYTLFLVAELFLMFKFARLGPSSLKTGR
                     YHFEQSSTTTQPAR"
     gene            9749..>10372
                     /gene="cydB"
                     /locus_tag="PN977_004398"
     CDS             9749..>10372
                     /gene="cydB"
                     /locus_tag="PN977_004398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HDI6044353.1"
                     /translation="MIDYEVLRFIWWLLVGVLLIGFAVTDGFDMGVGMLTRFLGRNDT
                     ERRIMINSIAPHWDGNQVWLITAGGALFAAWPMVYAAAFSGFYVAMILVLASLFFRPV
                     GFDYRSKIEETRWRNMWDWGIFIGSFVPPLVIGVAFGNLLQGVPFNVDEYLRLYYTGN
                     FFQLLNPFGLLAGVVSVGMIITQGATYLQMRTVGELHLRTRATAQVAA"
BASE COUNT         2494 a         2447 c         2578 g         2853 t
ORIGIN      
        1 tgtgcagcct ttcaggttca acacgccgcc aggtccaggc aggaagtagg gatcggattt
       61 ctcatacagt gtgtgtgcag ccgctgacgt ttcggtaaat ttacgcttcg gattaaatgt
      121 cttcagcgta aagccgttgt catcaatgat gcccatcgca cagtcagagc ctgagccggg
      181 cgttgcggcg attgccgcgc attcaaggat tttcccacaa tgcagcgcca gaccttcatc
      241 aaagccttgc atgatcggga gcgcagcaaa gcaggccggg tcgtaagcgc gcccgcccag
      301 caccacttgc gcgccttctt tcagcgcccg ctggaagggt tcgatgccca tttgcgcgac
      361 gatatacgta ctttcgtcaa tcgcgtcgtg ggttaacggc gggacaaaat ccagcgccgt
      421 gattttgccg ttatccagcg cctggtggac gatggctttg tcaacgtctg ccgggatcag
      481 cgccatcgag aacgcgagtt gctcttcccg ggcaatctca tgaattattt ctcggcacca
      541 ctccaggtgt ggagcggcac cagaaccgcc ggccgttccg atcaccaccg gtatattatt
      601 ttgcacgcct gcggtgatca tatagcgcaa atcacgcttg acccctgcgc gatcggtaaa
      661 cggtttgccc gccccgaggt agtgggggcc aggatcggaa gagcctgcat caacggcgat
      721 cagatcgggc gattctgcca tcgctttgcg gaagctctct tccgggaatc catacccgag
      781 gattgccgtt ggcgataaga ctttaaaaga acgagccatt tgttaatcct tagcctgcaa
      841 cagcgcgata gtacgattca tttcgttgaa gacgatatcg aggccttcgt caaagcccat
      901 ccctggtttt accagcatac gcatggggcg agcggcaagg gcgacgtgga cacaggtgcg
      961 ggcactgaca tcagtttcat tgcaggtgcc gccctggtac gcttccatgc tgtggctgtt
     1021 gcaataaaga actgcatcga cgatgttgtg aatactgccc agatccgggg ttttgatttg
     1081 caccatgtgg caactggcgg catcagtgaa atcaacaata tcctggtagg tgttacacca
     1141 ttcatcggcc acaattttca cgccggaacc gaggcgcgtc agctctttgg taatcgcggt
     1201 cagcaggcga atttgatcgg gcttgttacc ggcatcgacc ggcccttcaa tgtagagcgg
     1261 caggccttgc gcttcttttt ccaggctggc gatgtattgc gcacagcgaa gcggatccat
     1321 atcgaagatc agaccgatag tgccgtatac atcgatgtgc agggtagggt ggtagcgtgc
     1381 gctggtgcgc ttgctcagaa tacgatccga cagccagcgg acatattcgc gcagtttttc
     1441 acctttaaag cccagtttct cttcgacgtt attaatcagt gcatggggca gcacgtcgat
     1501 gcccttaagg atcatcttat cgacggcgat atatcgatcg tccccgctct gaccaaataa
     1561 tggaatggat tccgccacgc gtggcaactg ccattcatca cagaccactt cagttttcag
     1621 acggccggtt gccagcgcgg tagcatcaag cagcgcctgt gataatccgt agcgcacggc
     1681 ggtatgcagc aagttgccgt caatacgcaa tttgtcgaag aaacgggcat tcggcaggaa
     1741 agcatccaca tcgcggccta ccagtaatgg cttgatatgg tcgttgagga acggaataaa
     1801 gtgctcagcg aggaacagtg gatcgcgacc acctgccccg gaatactgta cggcagcaca
     1861 gtcacctacg gcgaccgcgc cgttttccag aatcaactgt accgatacgc actccccggc
     1921 ctgacgaacg gcgttaaacc cctgtgttac cggctccccg gtataaaaaa agccgtcatg
     1981 acccgctccg ttttttatcg cctgctggtc atcgaaatag aatgaggagt agccagcggt
     2041 gaacagagcc tgttttattt tcattatggt cttcctatta atttactgtg ggatacagca
     2101 ttgatgtcat ctacaaccat ctggaatgtc ggttggcgtc cttcaaagcg tgcgcgttcc
     2161 gcaacgtaat cgtggtggag gtcgagaata tcttttggca atggaacggc tccggcttcc
     2221 agtacgcgta tcgcgccggt gttgtcacgg accggaagaa ttttgcccgc gttgcaggcg
     2281 gctggcgcaa acggcacatc cagtacgccc gcttcaaatg ccagcaccgt accgcgcgca
     2341 atatcaccat tacccagttc gaacacttta ttcagtaccg cgcgaacctc gctcttaatc
     2401 agttcaattt ccagttcaac cgcagggcac ggcgggaatt tctgctcgtt gaccatgttg
     2461 agcatctggc gggaggcttt cagcccctga atgttggcag cggcagtcgg gataccccag
     2521 gcttcgtgcg gacttttggt gatgactttg gtggcaccag acatccccgc gacggctgcg
     2581 ccccaggaga taatagcgaa cgctttcgat tcgtcttccg gaaagccgcc catccactgg
     2641 tgaaacacgg tacttaattc atagtcggta tagccatagc tctgaaaata ttcgtgtgca
     2701 agttcacgca gagactggat cgccgcaaca tcctgcgtca ggctgccgac ctgtccataa
     2761 ccaacggtga tagatttcac accctgttcc agcgccagca agccttcgat tatcgcgata
     2821 gagtgcgaga taaacggcgg gatcagggta cccgttagtg ggccaaacgg ttcgcggttg
     2881 atgcggatcc cgtgttcttc atacatcccc attaaacggt cgcagtactg ccagtcgcgg
     2941 atcgattttt ccagcgttac gcgcttggca tacggaatat tgtaggaaat gccgccgcct
     3001 tcataactgg taaagccgct ggccatcgag atttcagcca gcagacgcgc atctggcgtg
     3061 ccgtggcgga tctgtagcgg tttttgcaaa gtttcggtga ggcgacggca agctgcgacg
     3121 ccgtggttaa cgaccggtaa accgttcagt ttagaggttc cggcttcgat ggatttttta
     3181 atccctacag ccgcttcttc gtaccgattc agacgggtat atgcgtcgat ggtgctcggc
     3241 agcagatcgc attcttcttg tagtgttttc agtagttcga tgtgttcgtc catcagcgcc
     3301 acgcctgcgc gcggttggct aagagtttta ccttccttgt ctgctttcag cagggcaaga
     3361 gagaagcgtt tatgctcagg aatggtttgt tggtatttaa cgccatcttc aaagttttca
     3421 acatccttgc cggtttccca ggttttcagt acctgttgcc gctcggtcat gaattcgtca
     3481 tgggttaatt tcttatttcg aagttccatt gattgcagcc ttaattgtta tagggttaag
     3541 cattgaatac tggtgcgcgc ggcggccagc ggatcgactc tggcgacgtt tgccagcagt
     3601 ggtaatagcc cccgggaatc gcggtaatat tcaaattcgg tcggtaagag aatgcgttta
     3661 ccgtcgtcgt ttaactcgcg gtatttcaac cagtgatgca tatcgaactg actggcacgc
     3721 gagagccatc cgccggttcc gacgacttta cgcacggttg tcaggtcacg tcccatctgt
     3781 aaatcgacat tgccgacaca ggtgcagacc tcttttttgg tgcccgcatg tctttcggtg
     3841 gcataaccca cacacagtcc ggccaggacg gtatcgaaat atttttcttc tgcattcgcg
     3901 ggtaagtagt cgggatgagc gactagatga cggaggtaat gataaaaagc gtccagctgt
     3961 gcgggttgct gagcaaaatt cacattgact aactctttcg cactttcacc aaccaccatt
     4021 gccgagacgc gcattcccag atcgccttcc accgtccgtt tcacgaaggg ttccggtacg
     4081 ccgtgtaaaa cggtgtcggg cgagagcgtg ttggcacagg cggaatagac atcggtggtt
     4141 gcgccaccca tgtcgatcag catgaactct ttccagctgt gatcgacatt actgattgcc
     4201 ttaaccagtt caaagactgt ccacggtgtc ggcattggtt cttcgcctgt tttatcaaca
     4261 acgacatcca gccctttccc tttaacgatg cgtttaagga atatgtcgca gatagcctgg
     4321 cgtgcagcta aagggttcgg atggtcaaga tcgggcagaa cgttgtcgac gatggtcagg
     4381 tctttatgcc ctaggatctc ctgcacctcg tcctgaatat cccggtttcc ggcataaata
     4441 atggcgcagt cgagtttgga ttctgccagc acacgggcgt tattcaggcc gtagctttct
     4501 tctccgccgt cggtgccgcc ggtgaacaag agaatatcgg gctgcgtttc ttccagtgcc
     4561 tggatatcac ggcggttcag cttgtaggag taatattgcg caattttagc ccccgcagag
     4621 tgagcggtta ctttcgcggt ttccagggtg atagaaggca ccaaccccat tgcggcaacc
     4681 gccagcccac ctttggcaga ggaggaatac ttcaatgcaa cctcaccgtt gttaaacagg
     4741 gggagggcat tatctacgtt taacaccagg ttcaggctgg agaaaaaccc cttcgccaga
     4801 tgatgggtgg tggttggggt cagtacgtgg ttaaccaacg ttaacgcgtc cccctcccgg
     4861 gcgaagaggg ctgctttggt ccacgtcgat ccaatatcga cagagacgat ttgcatcaga
     4921 ttgcctcttc aagaatacga gtatcaacgt catgacgttg gttgatatcg tgggccatca
     4981 gctgacaaac atcttctaag tcatgacttg gggcaaagac gcgatcaaat cccatctctt
     5041 tgaatttggt ttccacgtca gcgaagtcat gttttccgac caccaggtta ccgcccacat
     5101 agagcaggat gtcccccagt ccacgctcga tgcagcgttc gcgcatgccc agacagtcga
     5161 tatcgccatg cccatagata gaggagacga caatcgcgtc agcgccagtt tcaatggccg
     5221 catcaatata ttcgtcctgg ctgaccatta cgcccagatt aatgactctg aagccatggt
     5281 tgctaaaaac gcggtccaga actttattgc ctactgcatg gcagtccgcg ccaataacgc
     5341 caatcacaag tgttgctttc ttcatgggta atcctttgta agacagagag taaaataagt
     5401 tacacacgtc gtatttatgg ccgttcataa cggcacaggt ttaatatcac aggagtgtct
     5461 ttatctgacc gtaatgttgc aattgatcta ttttcaaaat gtctttcggg aaatgattgt
     5521 ttaaagtatg ttgttaaatt ctcatgttaa ttcagtgtgt tatgtcaact aatgtcatga
     5581 atgttaaatt tttaaaatta acttgatttg gtgtattgag gtatttgtga tctacttcta
     5641 atttgcattt tagtgatatg ggtttagcca tgagtaaatg catttcataa aatttaagtc
     5701 gaatttcact gcaattcatc tctgcttaat gtaaaaataa tgttgaaaat aacaaatggt
     5761 taatgattca tgcgaattat gaccttagtt agctcttttg atttttcgta aaaaagagag
     5821 gtacatgaag gtgctaccag tatgagtaac gttaatcaaa ggttttatta cctctggcaa
     5881 taacgcaggc cattttcgcg actgggtgga tttaatcagt ataatcctca tacaatagtt
     5941 cacgattaac tgaaccgtta acggggattt ggtgttgaaa tttagaaatg gcggagtcaa
     6001 ttaatgaaga caatatcgaa aaaatgacag tgtcagaatt aatttcaatt aatccttctc
     6061 gttgttgacc aaaaaagata tttatcttga tttcatttgt actacggcaa tcaatatgta
     6121 gtaatttttg atcgctgaga ttttctgttt cataaatgtt tttggcgata gtcggatgta
     6181 ttgatgccag acatctgctc agataagcca gatcctttag ggaacacccc gctgagtaga
     6241 ggatgtcaga gaatttttgt gtgtcaactg ttgtctctga agcgtatgtt gcattgacag
     6301 gaagggtgtt gaaagagatt tgagatggga tcattattta aatctttccg tgagtaataa
     6361 aaattaatcc ccaggcattg tttagcaata tttgccacag ggaatatata aaaattaaag
     6421 tatttgattg gcatcagtta actgatcaat aaccagtgcc agattggtaa acattttgaa
     6481 aaagcgatac tggctcggaa tatctctttc aaagacgaaa cattcaatgt cattgtttga
     6541 aaaataaagg actagtgttg ctgattcgtc aggaaaaata tggaaatata tctggttacc
     6601 agaaggagga tagtccagaa gaagggtatc tttgcccgtc acatacctgt tgtggtcgtg
     6661 gatgctacgc tcacacttca tcagaggtgt catgatactc atgtattcat ctgatgttaa
     6721 tatattaaaa tcaccatcca ttttaaagaa acgataaagt aacagtacat cctcttgcgt
     6781 aaccggttcg tttgttttta gagggaaata agtgggaacc agaagatgag gttctaatct
     6841 attttgttga gcgctataac taaatttgaa cataaattcc ggatctctca tcaattgagc
     6901 gacataatta tgcctggctt caaaaatttt aacttttatc aggttaaata agtctgtttc
     6961 gtcatcctgt attaaaagac attggccata gcagccaggc tttttttcat tataagcaga
     7021 aacggttatt ctgtcaggag tgtcaacgtt atatccaaaa gccagatagt gaccgtcctc
     7081 atcgagattt gtttccaggt tatttaaatt tatcacatga agtgtttgca atgctgattc
     7141 aatgagctgt tgactctctg aggtaaatgt ttcgcgctgc gttgtataaa gtgtatcgat
     7201 tccaacgact atccgatatg ggtcaaggat ggcggcaatc atctttatta cctgcacagc
     7261 cacgggtgat gtcattgatt cgttatctgt ttttagagga tcattagatg gccaacctga
     7321 gaagcgattg acagccgaac cgatattatc aggactcata gcagcaacct cattgtaatc
     7381 aagcgaggcg ctgagcgtac tcggctatag aaaaactaac ttgcaaaaat cgctcatttt
     7441 tcatttatca ggctaaagtg gtctctgttc actcagaaac aatcactccc gctaagtcat
     7501 acctgtttca tcgatacatt taagtgatta ttactaatga aacaataaca taacgtgctt
     7561 acggccggtg ctcataaagg caacaatcaa ttaactttga taaaaatatg ttatgtaaaa
     7621 tagcaacaaa ttggctaatc taatcaattg gtattataaa aagtggataa ggcggttctg
     7681 ttaaataaaa cactggtttt ttatctatac cttttcattg taataattgt gttgcataaa
     7741 tatttcacaa taaattaaca ataaaatcac caatgatcta tggtttatac ataattaaca
     7801 tatttggttg gttttgatat aaatcaattt ttaattcttg aaaaaaatct gcaaaacacg
     7861 ataaattgtc gtagatcaaa ttggtgagat cgtgacaatt tcgctataaa ttgatcactg
     7921 tcgaaaaatg caaatttgct caataaaaac ctgtttattg taaggatttt gcggcgtaat
     7981 ataattacgg gatcaatttg gtttattcat taacatattt gtaacttttt tatcattgtc
     8041 ttttcctctt ttgatgaaat ggcaacgata gaggcgagca aataattttg tattggggca
     8101 tgcgtgtgac cctttctaac ggggttcact ctcggagtct tcatgcgatg agcaaggagt
     8161 catgatgtta gatatagtcg aactgtcgcg cttacagttt gccttgaccg cgatgtacca
     8221 cttccttttt gtgccactga cgctcggtat ggcgttcctg ctggccatta tggaaacggt
     8281 ctacgtcctt tccggcaaac agatttataa agatatgacc aagttctggg gcaagttgtt
     8341 tggtatcaac ttcgctctgg gtgtggctac cggtctgacc atggagttcc agttcgggac
     8401 taactggtct tactattccc actatgtagg ggatatcttc ggtgcgccgc tggcaatcga
     8461 aggtctaatg gccttcttcc tcgaatccac ctttgtaggt ctgttcttct tcggttggga
     8521 tcgtttgggt aaagttcagc atatgtgtgt cacctggctg gtggcgctcg gttcaaacct
     8581 gtccgcactg tggattctgg ttgcgaacgg ctggatgcaa aacccaatcg cgtccgattt
     8641 caactttgaa actatgcgta tggagatggt gagcttctcc gagctggtgc ttaacccggt
     8701 tgctcaggtg aaattcgttc acactgtagc gtctggttat gtgactggcg cgatgttcat
     8761 cctcggtatc agcgcatggt atatgctgaa aggtcgtgac ttcgccttcg ctaaacgctc
     8821 ctttgctatc gctgccagct tcggtatggc tgccgttctg tctgttattg ttctgggtga
     8881 tgaatctggt tacgaaatgg gcgacgtgca gaaaaccaaa ctggctgcta ttgaagccga
     8941 gtgggaaacg caacctgcgc ctgctgcctt tactctgttc ggcattcctg atcaggaaga
     9001 ggagacgaac aaatttgcga tccagatccc ttacgcgctg ggcatcattg caacgcgttc
     9061 cgtggatact ccagttatcg gcctgaaaga actgatggtg cagcatgaag aacgcattcg
     9121 taacgggatg aaggcgtact ctctgctcga gcaactgcgt tctggttcta ccgaccaggc
     9181 ggttcgtgac cagttcaata gcatgaagaa agacctcggt tacggtctgc tgctgaaacg
     9241 ctatacgcca aacgtggctg atgcgactga agcgcagatt caacaggcaa ccaaagactc
     9301 cattccgcgt gtagcgccgc tgtacttcgc gttccgtatc atggtggcgt gtggcttcct
     9361 gctgctggca atcatcgcgc tctctttctg gagtgtcatc cgcaaccgca ttggcgagaa
     9421 aaaatggctg ctgcgcgccg cgctgtacgg tattccgctg ccgtggattg ctgtagaagc
     9481 gggctggttt gtggctgaat atggccgcca accgtgggct atcggtgaag tgctaccgac
     9541 agctgtggcg aactcgtcac tgaccgcagg cgatctcatc ttctcaatgg tgctgatttg
     9601 cggcctgtat accctgttcc tggtggcaga attgttctta atgttcaagt ttgcacgcct
     9661 cggcccaagc agcctgaaaa ccggtcgcta tcactttgag cagtcttcca cgactactca
     9721 gccggcacgc taagacagga gtcgtcaaat gatcgattat gaagtattgc gttttatctg
     9781 gtggctgctg gttggcgttc tgctgattgg ttttgcagtc actgacggtt tcgacatggg
     9841 ggtgggcatg ctcacccgtt tcctcggtcg taacgacacc gagcgtcgaa ttatgattaa
     9901 ctccattgca ccacactggg acggtaacca ggtttggctg atcaccgcgg gcggcgcact
     9961 ctttgctgcc tggccgatgg tctatgccgc tgcgttctcc ggcttctatg tggcgatgat
    10021 cctcgtgctg gcgtctttgt tcttccgtcc ggtcggtttt gactaccgct ccaagattga
    10081 agaaacccgc tggcgtaaca tgtgggactg gggcatcttc attggtagct tcgttccgcc
    10141 gctggtgatt ggtgtagcgt tcggtaacct gttgcagggc gtaccgttca acgttgatga
    10201 atatctgcgt ctgtactaca ccggtaactt tttccagctg cttaacccgt tcggcctgct
    10261 ggcaggcgtg gtgagcgtag ggatgatcat tactcagggc gcaacctatc tgcaaatgcg
    10321 taccgtgggc gaactgcacc tgcgtacccg tgcaacggct caggtggcgg ca
//
LOCUS       DAMZFT010000165        10367 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.165, whole genome shotgun sequence.
ACCESSION   DAMZFT010000165 DAMZFT010000000
VERSION     DAMZFT010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10367)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10367)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10367
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.165"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(<1..835)
                     /gene="modF"
                     /locus_tag="PN977_004399"
     CDS             complement(<1..835)
                     /gene="modF"
                     /locus_tag="PN977_004399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter ATP-binding protein
                     ModF"
                     /protein_id="HDI6044354.1"
                     /translation="MSSLQILQGTFRLGDTKTLQLPRLTLNAGDSWAFVGSNGSGKSA
                     LARALSGELPLLKGERQSQFSHITRLSFEQLQKLVSDEWQRNNTDMLSPGEDDTGRTT
                     AEIIQDEVKDTPRCMQLAQQFGITALLDRRFKYLSTGETRKTLLCQALMSEPDLLILD
                     EPFDGLDVASRQQLAELLASLHQSGITLVLVLNRFDEIPEFVQFAGVLADCTLAETGA
                     KEELLQQALVAQLAHSEQLEGVQMPEPDEPSARHALPANEPRIVLNNGVVSYNERPIL
                     NN"
     gene            complement(903..1691)
                     /gene="modE"
                     /locus_tag="PN977_004400"
     CDS             complement(903..1691)
                     /gene="modE"
                     /locus_tag="PN977_004400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum-dependent transcriptional regulator"
                     /protein_id="HDI6044355.1"
                     /translation="MQAEILLTLKLQEKLFADPRRISLLKHIALSGSISQGAKDAGIS
                     YKSAWDAINEMNQLSEHILVERATGGKGGGGAVLTRYGQRLIQLYDLLAQIQQKAFDV
                     LSDDDALPLNSLLAAISRFSLQTSARNQWFGTITARDHDDVQQHVDVLLADGKTRLKV
                     AITAQSGARLGLDEGKEVLILLKAPWVGITQDEAVAQNADNQLPGIISHIERGAEQCE
                     VLMALPDGQTLCATVPVNEATSLQQGQNVTAYFNADSVIIATLC"
     gene            1820..1969
                     /gene="acrZ"
                     /locus_tag="PN977_004401"
     CDS             1820..1969
                     /gene="acrZ"
                     /locus_tag="PN977_004401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux pump accessory protein AcrZ"
                     /protein_id="HDI6044356.1"
                     /translation="MLELLKSLVFAVIMVPVVMAIILGLIYGLGEVFNIFSGVGKKDQ
                     PGQNH"
     gene            2136..2909
                     /gene="modA"
                     /locus_tag="PN977_004402"
     CDS             2136..2909
                     /gene="modA"
                     /locus_tag="PN977_004402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HDI6044357.1"
                     /translation="MARKWLNLFTGAALSFAVAGNALADEGKITVFAAASLTNAMQDI
                     ATQYKKEKGVDVVSSFASSSTLARQIEAGAPADLFISADQKWMDYAVDKKAIDTASRQ
                     TLLGNSLVVVAPKASEQKDFTIDSKTNWTSLLNGGRLAVGDPEHVPAGIYAKEALQKL
                     GAWDTLSPKLAPAEDVRGALALVERNEAPLGIVYGSDAVASKGVKVVATFPEDSHKKV
                     EYPVAVVEGHNNATVKAFYDYLKGPQAAEIFKRYGFTTK"
     gene            2909..3598
                     /gene="modB"
                     /locus_tag="PN977_004403"
     CDS             2909..3598
                     /gene="modB"
                     /locus_tag="PN977_004403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HDI6044358.1"
                     /translation="MILTDPEWQAVLLSLKVSSLAVLFSLPFGIFFAWLLVRCTFPGK
                     ALLDSVLHLPLVLPPVVVGYLLLVSMGRRGFIGERLYDWFGLSFAFSWRGAVLAAAVM
                     SFPLMVRAIRLALEGVDIKLEQAARTLGAGRWRVFFTITLPLTLPGIIVGTVLAFARS
                     LGEFGATITFVSNIPGETRTIPSAMYTLIQTPGGESGAARLCIISIALAMISLLISEW
                     LARISRERAGR"
     gene            3601..4659
                     /gene="modC"
                     /locus_tag="PN977_004404"
     CDS             3601..4659
                     /gene="modC"
                     /locus_tag="PN977_004404"
                     /EC_number="7.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum ABC transporter ATP-binding protein
                     ModC"
                     /protein_id="HDI6044359.1"
                     /translation="MLELNFSQTLGNHCLTINETLPANGITAIFGVSGAGKTSLINAI
                     SGLTRPQKGRIVLNGRVLNDADKGICLTPEKRRVGYVFQDARLFPHYKVRGNLRYGMA
                     KSMVDQFDKLVALLGIEPLLDRLPGSLSGGEKQRVAIGRALLTAPELLLLDEPLASLD
                     IPRKRELLPYLQRLTREINIPMLYVSHSLDEILHLADRVMVLENGQVKAFGALEEVWG
                     SSVMNPWLPKEQQSSILKVTVLEHHPHYAMTALALGDQHLWVNKLDEPLQAALRIRIQ
                     ASDVSLVLQPPQQTSIRNVLRAKVVNSYDDNGQVEVELEVGGKTLWARISPWARDELA
                     IKPGLWLYAQIKSVSITA"
     gene            complement(4660..5478)
                     /gene="ybhA"
                     /locus_tag="PN977_004405"
     CDS             complement(4660..5478)
                     /gene="ybhA"
                     /locus_tag="PN977_004405"
                     /EC_number="3.1.3.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyridoxal
                     phosphate/fructose-1,6-bisphosphate phosphatase"
                     /protein_id="HDI6044360.1"
                     /translation="MTTRVIALDLDGTLLTPKKTLLPSSIEALARAREAGYRLIIVTG
                     RHHVAIHPFYQALALDTPAICCNGTYLYDYHAKTVLEADPMPVNKALQLIEMLNEHHI
                     HGLMYVDDAMVYEHPTGHVIRTSNWAQTLPPEQRPTFTQVASLAETAQQVNAVWKFAL
                     THDDLPQLQHFGKHVEHELGLECEWSWHDQVDIARGGNSKGKRLTKWVEAQGWSMENV
                     VAFGDNFNDISMLEAAGTGVAMGNADDAVKARANIVIGENTTDSIAQFIYSHLI"
     gene            5633..6628
                     /gene="pgl"
                     /locus_tag="PN977_004406"
     CDS             5633..6628
                     /gene="pgl"
                     /locus_tag="PN977_004406"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HDI6044361.1"
                     /translation="MKQTVYIASPESQQIHVWNLNHEGALTLTQVVDVPGQVQPMVVS
                     PDKRYLYVGVRPEFRVLAYRIAPDDGALTFAAESALPGSPTHISTDHQGQFVFVGSYN
                     AGNVSVTRLEDGLPVGVVDVVEGLDGCHSANISPDNRTLWVPALKQDRICLFTVSEDG
                     HLVAQDPAEVTTVEGAGPRHMVFHPNEQYAYCVNELNSSVDVWELKDPHGNIECVQTL
                     DMMPENFSDTRWAADIHITPDGRHLYACDRTASLITVFSVSEDGSVLSKEGFQPTETQ
                     PRGFNVDHSGKYLIAAGQKSHHISVYEIVGEQGLLHEKGRYAVGQGPMWVVVNAH"
     gene            complement(6669..7622)
                     /locus_tag="PN977_004407"
     CDS             complement(6669..7622)
                     /locus_tag="PN977_004407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308823.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDI6044362.1"
                     /translation="MKHELSSMKAFVILAESSSFNNAAKLLNITQPALTRRIKKMEED
                     LHIQLFERTTRKVTLTKAGKRLLPEARELIKKFDETIFNIRDMNAYHRGMVTLACIPT
                     AVFYFLPLAIGKFNELYPNIKVRILEQGTNNCMESVLCNESDFGINMNNVTNSSIDFT
                     PLVNEPFVLACRRDHPLAKKQLVEWQELVGYKMIGVRSSSGNRLLIEQQLADKPWKLD
                     WFYEVRHLSTSLGLVEAGLGISALPGLAMPHAPYSSIIGIPLVEPVIRRTLGIIRRKD
                     AVLSPAAERFFALLINLWTDDKDNLWTNIVERQRHALQEIG"
     gene            7806..8858
                     /locus_tag="PN977_004408"
     CDS             7806..8858
                     /locus_tag="PN977_004408"
                     /EC_number="5.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalomesaconate tautomerase"
                     /protein_id="HDI6044363.1"
                     /translation="MKKIPCVMMRGGTSRGAFLLAEHLPEDQTQRDKILMAIMGSGND
                     LEIDGIGGGNPLTSKVAIISRSSDPHADVDYLFAQVIVHEQRVDTTPNCGNMLSGVGA
                     FAIENGLIAATSPVTRVRIRNVNTGTFIEADVQTPNGVVEYEGSARIDGVPGTAAPVA
                     LTFLNAAGTKTGKVFPTDNQIDYFDDVPVTCIDMAMPIVIIPAEYLGKTGYELPAELD
                     ADKALLARIESIRLQAGKAMGLGDVSNMVIPKPVLISPAQKGGAINVRYFMPHSCHRA
                     LAITGAIAISSSCALEGTVTRQIVPSVGYGNINIEHPSGALDVHLSNEGQDATTLRAS
                     VIRTTRKIFSGEVYLP"
     gene            8934..10367
                     /locus_tag="PN977_004409"
     CDS             8934..10367
                     /locus_tag="PN977_004409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion permease"
                     /protein_id="HDI6044364.1"
                     /translation="MNKKSLWKLILILAIPCIIGFMPAPAGLSELAWVLFGIYLAAIV
                     GLVIKPFPEPVVLLIAVAASMVVVGNLSGGEFKTTAVLSGYSSGTTWLVFSAFTLSAA
                     FVTTGLGKRIAYLLIGKIGSTTLGLGYVTVFLDLVLAPATPSNTARAGGIVLPIINSV
                     AVALGSEPEKSPRRVGHYLMMSIYMVTKTTSYMFFTAMAGNILALKMINDILHLQISW
                     GGWALAAGLPGIIMLLVTPLVIYTMYPPEIKKVDNKTIAKAGLAELGPMKIREKMLLG
                     VFVLALLGWIFSKSLGVDESTVAIVVMATMLLLGIVTWEDVVKNKGGWNTLIWYGGII
                     GLSSLLSKVKFFEWLAEVFKNNLAFDGHGNVAFFVIIFLSIIVRYFFASGSAYIVAML
                     PVFAMLANVSGAPLMLTALALLFSNSYGGMVTHYGGAAGPVIFGVGYNDIKSWWLVGA
                     VLTILTFLVHITLGVWWWNMLIGWNML"
BASE COUNT         2433 a         2494 c         2722 g         2718 t
ORIGIN      
        1 ggttattaag aatggggcgt tcgttataag aaaccactcc attgttcagc acaatgcgcg
       61 gttcgttggc gggtaaggcg tgacgtgctg aaggttcatc cggctccggc atttgcacac
      121 cttcaagctg ttcgctatgt gccagttgcg cgacgagtgc ttgctggagc agttcctctt
      181 tagcgccagt ttccgctaac gtacaatccg ccagcacgcc agcaaactgg acaaattctg
      241 ggatctcatc gaagcgattg agcaccagta ccagagtaat accggactga tgtaacgagg
      301 cgagtagctc agccagctgc tgacgtgagg caacatccag gccatcgaac ggctcatcaa
      361 gaatcaacaa gtcaggctcc gacatcagcg cctgacacag cagggttttt cgcgtctcgc
      421 cagtggaaag gtatttaaag cgtcggtcga ggagggcggt aataccgaac tgctgcgcca
      481 gttgcatgca acgcggagta tcctttactt catcctgaat gatctcagcc gtggtacgcc
      541 cggtgtcatc ttcgccagga ctaagcatat cggtgttatt ccgctgccat tcgtcgctga
      601 cgagcttttg caattgctcg aaggagagac gagtgatgtg ggaaaactgg ctttgccgtt
      661 cacctttcaa aagcggaagt tcccccgaca gcgcgcgggc cagcgccgat ttcccgcttc
      721 cattcgaacc gacaaacgcc caactatcac ccgcgtttaa cgttagccga ggcaattgca
      781 gcgtttttgt gtcgccaaga cgaaacgtgc cttgcaaaat ttgcaacgat gacattttat
      841 atcccatttt gtgcagcgat tactgacagg gatacgtgtt tcataacaaa ttgtcaacac
      901 gcttagcaca gcgtggcgat aatcacgctg tcggcattaa agtaggccgt gacattctgt
      961 ccttgctgaa gagaagtcgc ttcatttacc ggcactgtgg cgcacagtgt ttgcccgtcg
     1021 ggtagcgcca ttaatacttc gcactgctct gcgccgcgct caatatgact aataataccc
     1081 ggtaattggt tgtcagcgtt ttgcgcgacc gcctcgtcct gagtaatccc tacccacggc
     1141 gcttttagca atatcaacac ttctttgcct tcatccagcc ccagacgtgc gccgctttgt
     1201 gcggtaattg cgactttcag gcgtgttttt ccatcagcca gtaagacatc aacatgctgt
     1261 tgaacgtcat catgatcgcg ggcggtgatg gtaccgaacc actggttacg ggcgctggtt
     1321 tgcagtgaaa aacgtgagat cgcggccagc aggctgttca gcggcagggc gtcatcgtca
     1381 cttaacacat caaaggcttt ttgctggatt tgcgccagta agtcatagag ctgaatcagt
     1441 cgctgaccat agcgggtcag tactgcgccg ccgccacctt taccgcctgt tgcgcgctcg
     1501 accagaatat gctcacttaa ctgattcatt tcgttaatgg catcccaggc gcttttatag
     1561 ctgataccgg catctttcgc tccctggcta atggaaccgg aaagcgcaat gtgttttagt
     1621 agcgaaatgc ggcgcgggtc ggcgaataat ttttcttgga gcttaagggt gagaaggatt
     1681 tcggcctgca taacaatatc ctggcaaaag tcttattgtg acggaaaacg aacgccacgc
     1741 aaagctgacc gcacaaaagg ggagtgcttt tctgtgctta gcggttagaa tagtctcatg
     1801 actatatctg gagttgacca tgttagagtt attaaaaagt ctggtattcg ccgtaatcat
     1861 ggtacctgtc gtgatggcca tcatcctggg tctgatttac ggtcttggtg aagtattcaa
     1921 catcttttct ggtgttggta aaaaagacca gcccggacaa aatcattgat tccctgaatg
     1981 cccgcttagt cgggcatttt ctttttctca acttcctgct tttcctgccg atattttttc
     2041 tcatctacct cacaaaggtt agcaataact gctgggaaaa ttccgaactt gccgttatat
     2101 tgacgcctac ataacgttag attaaggggt taccaatggc tcgtaaatgg ttgaacttgt
     2161 ttaccggggc ggcgctctct ttcgctgttg ctggcaatgc actggcagat gaagggaaaa
     2221 tcacggtgtt cgccgccgca tcactgacta acgcaatgca ggacattgct acgcagtata
     2281 aaaaagagaa aggcgtggat gtggtttctt ctttcgcttc gtcatctact ttggcccgtc
     2341 agattgaagc gggtgcgcct gcggatctgt ttatttctgc cgatcagaaa tggatggatt
     2401 atgcggttga taaaaaagcg atcgatacgg cttcgcgcca gacactgctc ggcaatagcc
     2461 tggtcgttgt agcaccgaaa gccagcgagc agaaagattt caccatcgac agcaaaacca
     2521 actggacttc gctgctgaat ggcggtcgcc tcgcggttgg tgatccggaa catgtccctg
     2581 ctggcattta tgcgaaagaa gcactgcaaa aactgggcgc atgggatacg ctctccccga
     2641 aactggctcc ggcggaagat gttcgtggcg cgctggcgtt ggtcgaacgc aatgaagcgc
     2701 cgctgggtat tgtttacggt tctgacgcag ttgccagcaa aggggtaaaa gtggttgcca
     2761 ccttcccgga agattcacat aaaaaagtgg aatatccggt tgctgttgtg gaagggcata
     2821 acaatgcgac agtgaaagca ttttatgatt atctgaaggg accgcaggca gcggaaatct
     2881 ttaaacgtta cggatttaca accaagtaat gatactgacc gatccagaat ggcaggcggt
     2941 tttactaagc ctgaaagttt cttccctggc tgtgctgttt agcctgccgt ttgggatctt
     3001 ttttgcctgg ttactggtgc gttgcacgtt tccgggcaaa gctctgctcg acagcgtact
     3061 gcatctaccg ctggtgttac cgcccgtggt cgtcggttac ctattattag tttcgatggg
     3121 acggcgcgga tttatcggtg aacgtctgta tgactggttt ggtctttctt tcgcgtttag
     3181 ctggcgtggt gcggttctcg ctgccgctgt catgtctttt ccgctgatgg tgcgggcgat
     3241 tcgtctggcg ctggaaggtg ttgatatcaa actggagcag gccgcaagaa cgttgggtgc
     3301 cgggcgctgg cgtgtcttct tcactattac cttaccgctg acgttgccgg gaattattgt
     3361 tggtacggta ctggcattcg ctcgttcgct cggtgaattt ggcgcaacca tcacctttgt
     3421 gtcgaacatt cctggtgaaa cgcgaaccat tccttctgct atgtataccc tgatccagac
     3481 cccaggtggg gaaagtggcg cggcgagact gtgtattatt tctattgcgc tggcgatgat
     3541 ctccctgttg atttcagaat ggctggccag aatcagccgt gaacgggcgg ggcgctaatc
     3601 atgctggaac tgaatttttc ccagacgttg ggcaaccatt gcctgacgat taatgaaacg
     3661 ctgcccgcca atggcatcac cgctattttt ggtgtctctg gtgccggaaa aacctcgctg
     3721 attaacgcca tcagtgggct gacgcgcccg caaaaagggc ggattgtcct caatggtcgg
     3781 gtactaaatg acgccgataa aggtatctgc ctgacgccag aaaagcgtcg cgtaggctat
     3841 gtgtttcagg atgcgcggct gttcccgcat tacaaagtgc gtggcaatct gcgctacggc
     3901 atggcgaaaa gcatggtcga tcagttcgat aagctggtgg cgcttttagg cattgaaccg
     3961 ttgcttgatc gtttaccagg cagcctgtcc ggaggcgaaa aacagcgcgt ggcgattggt
     4021 cgggctttgc tgacagcacc ggaattgctg ttgctggatg aaccgctggc gtcactggat
     4081 attccgcgta aacgcgaact gttgccttat ctgcaacgtc tgacgcggga aatcaacatt
     4141 ccgatgttgt atgtcagcca ttcgctggat gagatcctcc atctggcaga cagagtgatg
     4201 gtactggaaa acggtcaggt gaaagccttt ggcgcgctgg aagaagtgtg gggcagtagc
     4261 gtgatgaatc cgtggctgcc gaaagagcaa caaagtagca ttctgaaagt gacggtgctt
     4321 gagcatcatc cgcattacgc gatgaccgcg ctggcgctgg gcgatcagca tttgtgggtc
     4381 aataagctgg acgaaccgct gcaagctgcg ctacgtatcc gcattcaggc ttccgatgtt
     4441 tctctggttt tacaaccgcc gcagcaaacc agcattcgta acgtattgcg ggcaaaagtt
     4501 gttaatagtt atgacgacaa cggccaggtg gaagtggagc tggaagtcgg cggtaaaacg
     4561 ctgtgggcgc gtatcagccc gtgggccagg gatgaactgg cgatcaaacc aggcctgtgg
     4621 ctgtacgcgc aaattaaaag tgtgtcgata accgcctgat taaatcaggt ggctataaat
     4681 gaactgggca atgctgtcgg tggtgttctc accaatcaca atgttggcgc gcgcttttac
     4741 tgcgtcatcg gcgttgccca tcgccacgcc tgtaccagcg gcttccagca tactaatatc
     4801 attgaagtta tcgccgaacg ccacgacgtt ttccatcgac cagccttgcg cctcaaccca
     4861 tttcgtcaaa cgtttacctt tgctgttgcc gccgcgtgca atatcaacct gatcgtgcca
     4921 ggaccattca cactccagtc ccagttcatg ttcgacatgc ttaccaaaat gctgcaattg
     4981 cggcaggtcg tcgtgcgtca gggcgaactt ccatacggcg ttaacttgtt gcgccgtttc
     5041 agccagagaa gcgacttgtg tgaaagtcgg acgctgttcc ggcggcaggg tttgcgccca
     5101 gttagacgtg cgaatgacat gcccggtcgg gtgctcatag accattgcat catcgacata
     5161 catcagaccg tgaatgtggt gttcattcag catctcaatg agttgcaggg ctttattaac
     5221 gggcattggg tccgcttcca gcacggtttt tgcatgataa tcatacaaat aggtgccatt
     5281 acagcaaata gcaggtgtat ccagcgccag cgcctgataa aaaggatgaa tagcgacgtg
     5341 atggcgacct gtgacgatga ttaatcgata gcctgcttcg cgagcacggg ccagggcttc
     5401 tatcgatgaa ggaagcaggg tctttttcgg ggtcaataag gtgccgtcta agtcgagagc
     5461 aatcacgcgt gtggtcatgg cgtattccag attaaggtta agaattttcg tctgcgcgaa
     5521 tggtacaccg ataccactat cggacaaaat tctgcgtttt aattcagcat tcaccgccaa
     5581 aagcgactaa ttttagctgt tacagtcagt tgctaaatgc aaaggagcat tcatgaagca
     5641 aacagtttat atcgccagcc ctgagagcca gcaaattcac gtctggaacc tgaatcatga
     5701 aggcgcactg acgctgacac aggttgtcga tgtgccgggg caggtgcagc cgatggtggt
     5761 cagcccggac aaacgctatc tctatgttgg tgttcgccct gagtttcgcg tcctggcgta
     5821 tcgtatcgcc ccggacgatg gcgcgctgac ctttgccgca gagtctgcgc tgccgggtag
     5881 tccgacgcat atttccaccg atcaccaggg gcagtttgtc tttgtcggtt cttacaatgc
     5941 gggtaacgtg agcgtaacgc gtctggaaga tggcctgcca gtgggcgtcg tcgatgtggt
     6001 cgaggggctg gacggttgcc attccgccaa tatctcaccg gacaaccgta cgctgtgggt
     6061 tccggcatta aagcaggatc gcatttgcct gtttactgtc agcgaggatg gtcatctggt
     6121 ggcgcaggac cctgcggaag tgactaccgt tgaaggggcc ggcccgcgtc atatggtatt
     6181 ccatccaaac gaacaatatg cgtattgcgt caatgagtta aacagctcag tggatgtctg
     6241 ggaactgaaa gatccgcacg gcaatatcga atgtgtccag acgctggata tgatgccgga
     6301 aaacttctcc gacacccgtt gggcggctga tattcatatc accccggatg gtcgtcattt
     6361 atacgcctgc gaccgtaccg ccagcctgat taccgttttc agcgtttcgg aagatggcag
     6421 cgtgttgagt aaagaaggct tccagccaac ggaaacccag ccgcgcggct tcaatgttga
     6481 tcacagcggc aagtatttga ttgccgccgg gcaaaaatct caccacatct cggtatacga
     6541 aattgttggc gagcaggggc tactgcatga aaaaggccgc tatgcggtcg ggcagggacc
     6601 aatgtgggtg gtggttaacg cgcactaacc gctgatttac ccggcgcagt ctctcctgcg
     6661 ccggtgtatt aacctatctc ctgtaacgca tgtctctggc gttcgacgat attggtccac
     6721 aaattgtctt tatcgtcagt ccacaaatta atcagcaagg caaaaaagcg ttcagctgcc
     6781 ggagaaagca cggcatcttt gcggcgaata atccccaatg tccgacgtat aacgggttcc
     6841 accagtggga taccaataat cgaagaatag ggcgcatggg gcattgccag gcctggaagc
     6901 gccgaaatcc ccagtcctgc ctccaccagt cctaatgacg tcgaaagatg acgcacttcg
     6961 taaaaccagt ccagcttcca gggcttgtcg gctagctgtt gttctatcaa cagtcggttg
     7021 ccgctggagg agcgtacgcc aatcattttg taacccacta actcctgcca ttctacaagc
     7081 tgctttttgg ccaatggatg gtcacgccga caggccagca cgaacggctc gttgaccagt
     7141 ggggtaaaat cgatggatga atttgtgacg ttgttcatgt ttatgccaaa gtctgattcg
     7201 ttacacagca ccgattccat gcaattattc gtgccttgtt ccagaatccg cactttaata
     7261 ttgggataca gctcattaaa tttaccgatt gccagcggta aaaaataaaa tactgcggtc
     7321 ggaatacacg ctaatgtcac cataccacga tgataagcat tcatatcacg aatattaaaa
     7381 atcgtttcat cgaatttctt tattaattcc cgcgcctccg ggagcaatct ttttcctgct
     7441 tttgttaacg taaccttgcg agttgtacgc tcaaatagtt ggatatgtaa atcttcctcc
     7501 atctttttta ttcttcgcgt taatgcaggt tgcgtaatat tgagtaattt agcggcattg
     7561 ttaaatgaac tggactctgc cagtatgaca aatgccttca tacttgataa ttcatgcttc
     7621 attattactc cggaaaatgg aagcgtcgat tttgggtggc tgaccgttaa aaattttaac
     7681 tgcatttaac caacttaaat taatgaaaaa atgttattaa tcgttgagct aaagtcatta
     7741 gagatgcttt gcccttaatg taaccatatc gcaataagtt atgtttttaa attgagggca
     7801 ttattatgaa aaaaataccc tgcgtgatga tgcgaggtgg aacctcgagg ggcgcgttcc
     7861 tgttagcgga acatctaccc gaagatcaaa cgcagcgcga taaaatattg atggcaatta
     7921 tgggttccgg taacgatctg gaaattgacg gtattggcgg cggtaatccg ctgaccagta
     7981 aagtcgccat tattagccgc tccagcgatc cgcatgcaga tgtcgattat ctgtttgctc
     8041 aagttatcgt ccatgagcaa cgtgtcgata ccacgcctaa ctgcggcaat atgctgtctg
     8101 gcgttggggc attcgccatt gaaaatggtt tgattgcagc gacttcgcca gttacccgcg
     8161 tacgtatccg caacgtcaat acgggtacgt tcatcgaagc tgatgtgcaa acgccaaatg
     8221 gtgttgtcga gtacgagggt agcgccagaa ttgacggcgt accgggtact gccgcaccgg
     8281 ttgcgctcac tttcctgaat gccgccggaa cgaaaaccgg aaaagtcttc ccgacagata
     8341 atcagattga ttattttgac gatgtcccgg tgacctgtat cgatatggcg atgccaattg
     8401 tcattattcc tgctgaatat ctgggaaaaa caggctatga attaccggca gagctggatg
     8461 ccgacaaagc attattagcc cgcattgaat ctatccgtct acaagcgggt aaagcaatgg
     8521 gcttaggcga tgtcagtaat atggttatcc ctaaacctgt acttatttct ccagcgcaga
     8581 aaggcggggc aattaatgtg cgttatttta tgccgcattc ttgccatcgc gctttggcga
     8641 taaccggtgc tattgctatt tccagtagtt gtgcattgga aggcaccgtc acccgacaaa
     8701 tcgtcccttc tgtaggatac ggcaatatca atattgaaca ccccagtggt gcgctcgacg
     8761 ttcatttaag taatgaaggt caggatgcca cgacgttacg cgcatctgtt attcggacga
     8821 ccagaaaaat attttccggt gaagtttatc ttccctgaaa aattttgttg tcgtgataat
     8881 gacaatcaat aaaggaattc tgtatgagtc atacagaaag aacaggatat taaatgaata
     8941 agaaatcgtt atggaagcta attctgatat tagcaatccc atgtattatt ggctttatgc
     9001 cagctccagc aggattaagc gaactggcgt gggtgctttt tggtatttac ctggcggcca
     9061 ttgtggggct ggttatcaag cctttcccgg aacctgtcgt actgttaatt gccgtcgccg
     9121 catcgatggt agtggtgggt aacttatcag gtggggaatt taaaaccacc gctgtattaa
     9181 gcggttactc ttccggtacc acctggctgg tgttttctgc gtttacttta agcgccgcgt
     9241 ttgtaaccac aggcttaggt aaacgtattg cctatctgct gattggtaaa attggtagca
     9301 ctaccctggg tctgggttac gttacggtat ttctcgatct ggtactggct ccggcaacac
     9361 cgtctaacac cgcgcgtgcg ggcggcatcg tgttaccgat catcaacagc gtggcagtgg
     9421 ctttgggatc agaaccggaa aaaagtccgc gtcgtgttgg acattacctg atgatgtcca
     9481 tttacatggt caccaaaacc accagctata tgttctttac cgcaatggcg gggaacattc
     9541 tggcgctgaa aatgatcaac gacattctgc acctgcaaat tagctggggt ggatgggcgc
     9601 tagccgccgg attgcctggc atcattatgc tgctggtcac cccgctggtg atttacacca
     9661 tgtatccgcc agaaattaag aaggtggata acaaaaccat cgccaaagcg ggccttgccg
     9721 aactgggacc gatgaaaatc cgcgaaaaaa tgctgctcgg tgtcttcgtg ctggcgctgc
     9781 tgggctggat tttcagtaag tcactggggg ttgatgaatc caccgtggca atcgttgtta
     9841 tggcgactat gctgctgctg ggtatcgtta cctgggaaga cgtggttaaa aataaaggcg
     9901 gctggaatac cttaatctgg tacggcggta ttatcggctt aagctcctta ttatcgaaag
     9961 ttaaattctt cgaatggtta gctgaagtct ttaaaaataa cctggcattt gatggtcacg
    10021 gtaacgttgc tttcttcgtt attattttcc tcagcatcat cgtgcgttat ttcttcgctt
    10081 ccggtagtgc ctatatcgtt gccatgttac cggtatttgc catgctggcg aacgtctccg
    10141 gcgcgccgtt aatgttaacc gcgctggcac tgttgttctc taactcctat ggcggcatgg
    10201 ttactcacta tggcggcgcg gcaggtccgg tcatctttgg cgtgggttac aacgatatta
    10261 aatcctggtg gttggtcggt gcggtactga cgatattaac cttcctggtg catatcaccc
    10321 tcggcgtgtg gtggtggaat atgctgatcg gctggaacat gctgtaa
//
LOCUS       DAMZFT010000166        10333 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.166, whole genome shotgun sequence.
ACCESSION   DAMZFT010000166 DAMZFT010000000
VERSION     DAMZFT010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10333)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10333)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10333
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.166"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(<1..155)
                     /locus_tag="PN977_004410"
     CDS             complement(<1..155)
                     /locus_tag="PN977_004410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDI6044365.1"
                     /translation="MGDSVTPEVEVLSNMIRQYFSQERSEEETIRALNHLRRVLHEVS
                     PFAQEPV"
     gene            complement(140..1372)
                     /locus_tag="PN977_004411"
     CDS             complement(140..1372)
                     /locus_tag="PN977_004411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001083460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HDI6044366.1"
                     /translation="MNNDFSAKKILTGEDVLCAAVHRIEWLFETFSSVCLSFSGGKDS
                     TVLLHLTADVARRKKRRFSVLFIDWEAQYQCTIAHILKMREMYRDVTETFYWVALPLT
                     TVNGVSQFQPEWICWEPGVEWVRQPPDDAITDMSYFPFYRYAMTFEEFVPAFSSWFAG
                     NRCGVAILTGVRADESLNRFVGLVSQRKLRYADDKPWTTASPEGFYYTLYPLYDWKAR
                     DIWIYNARTRAIYNPLYDLMYRAGVPLRNMRVCEPFGPEQRKGLWLYHVLEPETWARM
                     CERVSGAASGALYANESGAYFALRKRITKPPHHTWRSYAMFLLDVMPERTAEHYRNKI
                     AVYLRWYQTRGFPDDIPDEQENDLGSRDIPSWRRICKTLIKNDFWCRTLSFSPNKPRH
                     YERYLQRMKERRKEWGIL"
     gene            complement(1362..1496)
                     /locus_tag="PN977_004412"
     CDS             complement(1362..1496)
                     /locus_tag="PN977_004412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000065644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HDI6044367.1"
                     /translation="MSNISLYCLPYSGGSAAMYYKWRNVLSDNITLKPLEPVGKGNEQ
                     "
     gene            complement(1543..1713)
                     /locus_tag="PN977_004413"
                     /pseudo
     CDS             complement(1543..1713)
                     /locus_tag="PN977_004413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002430641.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
     gene            complement(1758..2147)
                     /locus_tag="PN977_004414"
     CDS             complement(1758..2147)
                     /locus_tag="PN977_004414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000647520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6044368.1"
                     /translation="MKARIKELEPYRALFIEKPVLAEHDDVIKQSEESETYSCLLRAP
                     AGLFLSFCLLWHYPIFPMLNTDTPEDILSLLGTLWPRGVTVRLPDMPAVQTTGLPGYL
                     FDRVRGQRCEIVRYVTSELFVGARLCK"
     gene            2806..4182
                     /gene="argH"
                     /locus_tag="PN977_004415"
     CDS             2806..4182
                     /gene="argH"
                     /locus_tag="PN977_004415"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001695885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HDI6044369.1"
                     /translation="MSTKLWGGRFDMPTNKLVEQYNATITLEQRLCPFDIQGSIVHAT
                     MLGRQGIITQDEANTIIRGLRQVSKEIEDGQFIFDTVDEDIHMAIERRMTEIIGPVGG
                     KLHTGRSRNDQTTVDSKMHMRAIIREIQEDITNLQKIIINKAENNINVIMPGYTHLQT
                     GQPILLSHWIMAYYWMLRRDWNRFEDLYQRMGECPLGAAALAGTTFPIDRNFTARELG
                     FDKPTENSIDSVSDRDHMVEFTAAAAMCFMHLTRLSEELILFSSQDFKFIELSDDFCT
                     GSSIMPQKKNPDVAEKMRGKGGRMYGNLMAMLTIMKGIPLAYNTDMSEDKEQVYDSMD
                     TLQASLRIMAPMIEKMVILAENTRAAAARGFSNATDMADYLVRKGIPFREAHHIVGSA
                     VNYCIKHKKMLEELTMEEFSTFDNKIEKDIYESISLEACIKARMSYGGTGPDAVKKQI
                     EIAKSLLK"
     gene            4247..5563
                     /locus_tag="PN977_004416"
     CDS             4247..5563
                     /locus_tag="PN977_004416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016153937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter"
                     /protein_id="HDI6044370.1"
                     /translation="MDALFFIQLFIVLACIGIGGKYGGMGLGAGGGLGVAILVLGFGL
                     KPSSPSITAMLIIICVISAVSILQAVGGLDYLVRVAERILRKKPQAITFVAPILTSVF
                     TLFCGTTYVAFSLYPVIAEVAAEAKVRPERALSATVIAASVAVAASPMSAATAGMLAI
                     LHEYAGITLGQILSIALPSFFMAAIVTSFSVYKRGKELEDDPEFQRRVAAGEYEFMHT
                     EQKKEYVAAPGARKGVAIFAIGVVLVLILGSFTELLPSWDGKRLSTPMVIQMIMLTAA
                     LLIMIVGKVPSNKFNSGSVFRAGLMGVVAILGVSWMTATFFDAYQAELINVFGNLVND
                     APLLFGFIVFLFSLVIMSPAATVAAIMPLGVTLGIPAPYLIAIFACTCGDFIIPGANQ
                     IGCVAFDRTGTTRIGRFVINHSYIRPGFVMVISQVVFAYLIAQVIL"
     gene            complement(5679..6995)
                     /locus_tag="PN977_004417"
     CDS             complement(5679..6995)
                     /locus_tag="PN977_004417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016153936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter"
                     /protein_id="HDI6044371.1"
                     /translation="MMLFVIQFAIVLTCIGIGGRFGGIGLGAAGGLGLAILTFGFGVP
                     PDSPPITVISIILAVITCIAILQAAGGLDLFVTIAEKILQKRPGAITFLGPAVAYLFT
                     AICGTGYVAFSIYPVIAEIAADARVRPERAMSMSVIAANFGLIASPVSAVVTGTIAVF
                     SGLHVSALDILLITVPGTILGCLVGCLFVYKRGHDLETDPEFQRRVAEGEFESVKTGE
                     RTISIISKTAKKALMIFISGIILVVVLGSVPELRPVWNTSAGVERMSIPTALQIIMLT
                     TACIIMMVCRISPSKLDSGSVFKAGLVGVVAIFGLSWMMSSFFEAWQDLFNNTFNDFH
                     NPVIFGVIVFVLSAVIYSPAATAVALFPAGVLMGYSTETLIALLPVTCGSFIIPGGAQ
                     IACVAFDRTGTTRVGKYVVNHSYMLPGLITVLASTIFCFLFSTILV"
     gene            7150..7806
                     /locus_tag="PN977_004418"
     CDS             7150..7806
                     /locus_tag="PN977_004418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016153935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HDI6044372.1"
                     /translation="MDKRKELFESIKDYLLDEANRKADGRIETETELATRFNTSRYKV
                     RQALSTLVQMGMLERAPRRGSLLKNVGQSSINDQLLMQFDLSGFDISEFTEARILVEC
                     AIIPLATRRIFPAMLSKLENELRLIEENADNPQIADQHDRDFHLLLLQASGNRVLQVF
                     SDVLIAYFEKTRESLPDNDCQYFLDTASKQREILSYIKKGDAQMASELMRVHLLEKKI
                     "
     gene            8785..9510
                     /gene="dcuR"
                     /locus_tag="PN977_004419"
     CDS             8785..9510
                     /gene="dcuR"
                     /locus_tag="PN977_004419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001067029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator DcuR"
                     /protein_id="HDI6044373.1"
                     /translation="MNNVLIVDDDILVADVIRRIVEQTPAFKCCGIALSLGQAKEIIS
                     VNKKLADLILLDLYINKDNGLDLLPFLHSIHCKSDVIVISAADDSATIKDSLHYGVSD
                     YLIKPFHISRLVDSLTRWKHKKTLLESQKIFNQSELDLLTHKIIPNTEKIRHLPKGLS
                     TDTLRIICRWIDEHQHKKFTLNELASGIKISRISCRKYIIWLVSCHILAFDFSCGVTG
                     RPAYRYWIRHQYYPYIKKYSEKP"
     gene            complement(10104..>10333)
                     /gene="ansB"
                     /locus_tag="PN977_004420"
     CDS             complement(10104..>10333)
                     /gene="ansB"
                     /locus_tag="PN977_004420"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708728.1"
                     /note="catalyzes the formation of aspartate from
                     asparagine, periplasmic; regulated by cyclic AMP receptor
                     protein (CRP) and also induced by anaerobiosis; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="L-asparaginase 2"
                     /protein_id="HDI6044374.1"
                     /translation="SVFDTLATAAKNGTAVVRSSRVPTGATTQDAEVDDAKYGFVASG
                     TLNPQKARVLLQLALTQTKDPQQIQQIFNQY"
BASE COUNT         3140 a         2156 c         2144 g         2893 t
ORIGIN      
        1 tccacaggct cctgtgcaaa agggctgacc tcatgcaaca ccctgcgcag atgattaagg
       61 gctcgtatgg tctcttcttc agatcgttcc tgactgaagt actgtcgaat catgttgctc
      121 aatacttcca cctcaggtgt cacagaatcc cccattcctt cctcctttct ttcatacgct
      181 gcagataacg ttcatagtgc cggggtttgt tcggactgaa ggagagggtc cgacaccaga
      241 aatcattctt tatgagtgtc ttacagatac gtcgccagga cgggatatcc cggctcccca
      301 ggtcattctc ctgttcatcc gggatgtcat ccgggaagcc ccgcgtctga taccagcgca
      361 ggtagacagc aattttatta cggtaatgtt ctgccgttct ttccggcatc acatccagca
      421 ggaacatcgc atagctacgc caggtatgat gaggtggctt cgtgatacgc ttacgcaggg
      481 caaaataggc accgctctca ttggcataaa gcgccccgct ggcagcaccc gacacccgct
      541 cacacatcct ggcccaggtt tccggctcca gaacatggta aagccacagt cccttacgct
      601 gttccgggcc aaaaggctca cagacccgca tgttgcgtaa cggcacgccg gcacggtaca
      661 tcaggtcata caggggattg tagatagctc gggttctggc gttatatatc catatatcac
      721 gagctttcca gtcatacaac ggatacaagg tgtaataaaa cccttcaggt gacgctgtgg
      781 tccagggttt atcatctgca tatctcagtt tgcgctgaga caccagtccc acaaagcgat
      841 tgagcgattc atcagcacga acaccagtca gtattgccac tccacaccgg ttaccggcaa
      901 accaggaaga aaatgccgga acaaattctt caaacgtcat ggcataccga taaaatggga
      961 agtacgacat atctgtaata gcgtcatctg gtggctgacg aacccactcc acacctggtt
     1021 cccagcatat ccattccggc tgaaactgag agacaccgtt taccgtagtc agggggagtg
     1081 ccacccagta aaaagtctcc gtcacatccc gatacatctc cctcatcttc agaatgtgtg
     1141 caattgtgca ctggtactga gcttcccagt caatgaataa tacagagaaa cgacgtttct
     1201 tcctgcgagc cacatcggcc gtaagatgga gcagcacagt agagtctttt cctccggaaa
     1261 aagacaaaca gacagaagaa aatgtctcaa acaaccactc aatacggtga acggccgcac
     1321 acaggacatc ctcacccgtc aggatttttt tcgcggaaaa atcattgttc attccccttc
     1381 cccacaggtt ctaaaggctt caaagtaata ttgtccgaca gcacgttacg ccatttatag
     1441 tacatagcgg cagaaccacc tgaatatggc aaacaataca aactgatatt actcatcctc
     1501 ccactccttg ttgtgtaata gaattcgttt aatttgatga tgttatgtca gaacgaaagc
     1561 taacaagata atttctatcg gtatagccaa cacactcgat ggaccccata tcaggataaa
     1621 acgctacagt ataaccgctg ccatacagag catggcggtc acgaacacgg gtaccgccag
     1681 ggtgatgaca tagtcacgta cgcgtaaacg taaattgttc gccataatga tcaatgcata
     1741 acaatgaacg ccagtcttta tttacaaaga cgcgcaccga caaataactc actggtaaca
     1801 tagcgtacta tttcgcatcg ctggccccgg acgcgatcaa acaaataacc aggtaagcct
     1861 gtggtctgca cggcaggcat atccggtaag cgaacagtaa ctccccgagg ccaaagagtg
     1921 cccaacagag acaaaatgtc ctcaggcgta tccgtattca acatgggaaa aatagggtaa
     1981 tgccagagca ggcagaaact gagaaacaat cctgcaggtg ctcttaaaag acaactgtaa
     2041 gtttctgact cttcagactg cttaatcaca tcatcatgct ccgccagcac cggcttttca
     2101 ataaacagcg cgcgatacgg ttccagttct ttaatccgtg ctttcactat caaccactat
     2161 cagcacgctg gtgcaatcgt ggaaatcaag accagactca atctgattgc caaaagcttc
     2221 acgaatagcc ccagttcttt acaaccattc agtttgaggg tatcgaagcc gatttcgtgc
     2281 agggttttaa taccgtcgat agcatttgcc ggacgatcgc cgccaaccct acgataattt
     2341 tatgtgctgt ccatcacttc tatcaacatc attttctgtc ctttgtacta cgaccatttc
     2401 ctgttatttg aatcaattca atatcgctag gataagataa ttcataaccg taaagtaacc
     2461 gacgccagac aagagttgat aacttagatg ttatctgacg cagaagaaaa gagagagttt
     2521 ttgactttta tcaaagaccc atttgaactc ttgttttcat gtttttgcat aattgaattt
     2581 attaaatata tttctcgtca aataacatgt taaacgtgag gtatatctcc atgcttatca
     2641 ttaaaacatt aacaaacatt atgatttaat gattaaaact aatggcaata taagtcaatt
     2701 taatcgccgc atttgttgac aattgccaac atcattatat aacctgatat taaatccagc
     2761 cggtacattt tttctaatta cttatgaata agcaggagga tgaggatgtc tacgaaatta
     2821 tggggcggac gttttgatat gccaacaaat aaactcgttg aacaatataa tgcaactatc
     2881 acgctggagc agcgcctatg cccctttgat attcaaggaa gcatagttca tgccacgatg
     2941 ttaggacgtc agggaataat cacacaggat gaagccaata caattattag aggtttgaga
     3001 caagtaagta aagaaattga agacggccaa ttcattttcg atactgtaga tgaagatatt
     3061 catatggcca ttgaaagacg gatgactgaa attattggcc ctgttggtgg taaactccac
     3121 acggggcgaa gtcgtaatga tcaaaccact gttgattcta aaatgcatat gcgagcaatt
     3181 atccgtgaaa ttcaagagga tattaccaac ctgcaaaaaa taataattaa caaagcagaa
     3241 aacaatatta atgtcatcat gccaggctat actcatttgc aaacaggtca gcccattctt
     3301 ttatctcact ggattatggc atattactgg atgttgcgtc gtgactggaa taggtttgaa
     3361 gatctgtatc aacggatggg agaatgccct ttgggggcag cggctctcgc cggtacgaca
     3421 ttccctattg atcgtaattt tacggctcgt gaacttggtt ttgataagcc aactgagaat
     3481 agtattgatt ctgtcagtga ccgcgaccat atggtcgaat tcaccgcggc agcagcgatg
     3541 tgttttatgc atctaactcg cctttcagag gaactgattt tattctctag ccaagacttt
     3601 aaatttattg aactttctga tgacttctgt acaggatcca gcatcatgcc gcagaaaaag
     3661 aaccctgatg tggcggaaaa aatgcgtggt aaaggtggga gaatgtatgg aaatctgatg
     3721 gccatgctga ctattatgaa aggcataccg ctagcgtata atacagacat gagcgaggat
     3781 aaagagcagg tctatgactc aatggatact ctacaggcca gcttaagaat aatggcacct
     3841 atgatcgaaa aaatggttat ccttgccgaa aatacgcgtg cagcagccgc tcgaggattc
     3901 tcgaatgcaa cagatatggc cgattatctg gtccgtaaag gtattccttt cagagaagct
     3961 caccatattg ttggtagtgc agtaaattac tgtattaaac ataaaaaaat gttagaagag
     4021 cttactatgg aagaattctc cacatttgat aataaaatag aaaaagatat ttatgaaagt
     4081 atttctctgg aggcttgcat taaggccagg atgtcttatg gtggaaccgg acctgatgct
     4141 gtcaaaaaac aaatagagat tgcaaaatca cttttaaaat agttaagaat tataatgttt
     4201 aagattcccc ggatattata tccgggaata tctttgaggt tgaaaaatgg acgctctatt
     4261 ttttatacaa ctatttattg ttctggcctg tattggaatc ggcggaaaat acggcggaat
     4321 ggggcttggt gctggaggtg ggttaggggt tgcaatattg gttttgggct ttggactgaa
     4381 accctcctct ccctccatta ctgctatgtt aattattatt tgtgttatta gtgctgtcag
     4441 tatattacaa gctgttggag gattagatta tttagtcagg gtcgcagaac ggatactccg
     4501 taagaaacca caggcgataa cctttgtagc gccaatatta acttcagtat ttacactatt
     4561 ttgtgggacg acttatgtcg ctttctcctt ataccctgtt attgctgaag tcgccgctga
     4621 agcaaaagta cgtccagaac gtgcattatc tgcaacggtc attgcagcca gtgtggcagt
     4681 ggctgccagc cctatgagtg cagcaactgc cgggatgctg gctatacttc atgagtatgc
     4741 agggatcacg ttagggcaaa tattatctat tgcccttcct tcgtttttta tggctgcaat
     4801 tgttaccagc ttttcagtct acaagcgtgg taaagaactg gaagatgatc ctgaatttca
     4861 aaggcgtgtt gcagcaggtg agtatgagtt catgcacacc gaacagaaaa aagaatatgt
     4921 cgctgcacca ggtgcaagaa aaggggttgc tatttttgcc ataggagtcg tgctggtcct
     4981 tattctcgga tcttttactg agcttcttcc gtcgtgggat ggaaaaagat tatcaacccc
     5041 aatggtcatc cagatgatta tgttgactgc agctttattg atcatgattg tggggaaagt
     5101 tccaagcaat aaatttaaca gtggctctgt gttccgtgct ggtttgatgg gggttgtggc
     5161 aatacttggt gtttcgtgga tgactgcaac attttttgat gcttatcagg cagagctgat
     5221 caatgttttt ggcaatcttg taaatgatgc gcctttgctg tttggcttta tcgtattctt
     5281 gttttctctg gtgatcatga gcccggctgc gacagtagct gcgattatgc cattaggagt
     5341 caccctgggt attcctgcac cttatcttat tgcaattttt gcctgtactt gtggtgattt
     5401 tattataccc ggagcgaatc agattggttg tgtggcgttt gacagaactg gtactaccag
     5461 aattgggcgc tttgttatca accatagcta tatacgtcct gggttcgtca tggtcatttc
     5521 ccaggttgtt tttgcctact taattgcgca ggttattctg taacattaag gaataaatga
     5581 agaggataac actgtggcat gacgaattag cccccttcag cgtttttgag gaagggggat
     5641 taagaaaata ttacacagct gtaagaatac ttctctgatt acactaatat agtggaaaaa
     5701 aggaagcaga agatagttga agccagaacg gtaatcagac cgggtaacat ataactgtga
     5761 tttactacat acttgccaac tctggttgtt cctgttctgt caaacgccac gcaagcaatt
     5821 tgtgcaccac caggaataat gaatgatccg cacgttaccg gaagcaaagc tatgagcgtc
     5881 tcagtcgaat atcccattaa tacgccagca ggaaataatg ccacagcagt tgctgcggga
     5941 ctgtaaatca cagcagaaag aacaaacaca atcacaccga atataactgg gttatgaaaa
     6001 tcattgaaag tgttattaaa taaatcttgc catgcttcaa agaaagaact catcatccat
     6061 gaaagaccaa atatggcaac aactccaacc agaccggcct taaaaacgga tcctgagtca
     6121 agtttcgatg gagaaatccg acataccatc ataatgatac atgctgtagt caacatgatg
     6181 atttgcaatg ctgttggaat actcatccgt tctacaccgg cactggtatt ccataccgga
     6241 cgtaattctg gaacagagcc taaaacaaca accaaaatga tcccagaaat aaaaatcatc
     6301 agggctttct tcgctgtttt agatataata ctgattgtac gttccccagt ttttactgac
     6361 tcaaattcac cttctgcaac tctgcgttga aactctgggt ccgtttcaag atcatggccc
     6421 cgtttataaa caaacaaaca accaacaaga caccccaaaa tagttcccgg tacagtaatc
     6481 aataaaatat ctaaagcaga aacatgtaat ccggaaaaga ccgcaatcgt tcctgtaacg
     6541 accgcactta ctgggctcgc tataagacca aagttggcgg caatgactga cattgacatg
     6601 gcgcgttctg gcctcactct tgcatcagct gcgatttcag caataacagg ataaatggaa
     6661 aaagcgacat aaccagttcc acatattgcc gtaaaaagat aagcaactgc tggcccaaga
     6721 aacgtgattg caccaggcct cttttgcagt attttttcag ctattgtcac gaaaagatca
     6781 agacctcccg ctgcctgcag gatagcaata catgtgataa cagcaagaat aatggaaatg
     6841 acagtgatag gcggggaatc aggcgggact ccaaaaccaa aagtcagaat tgcgagccct
     6901 aatccccccg ccgcccctaa gccgattcca ccaaagcgcc cacctatccc aatgcatgta
     6961 agtactattg caaactgaat tacgaataac atcataacca atccctttcg tcaacaaata
     7021 gcttaatatc atgcacataa tgtgcgaata aacatgataa ataaatttgt tatgatagta
     7081 tataaagaaa tataatttaa tatattattt atagttatga atagatagga gctgtaatca
     7141 gagtatatta tggataaaag aaaagaacta tttgaatcta ttaaagatta tttgcttgat
     7201 gaagctaatc gtaaagccga tggccgtata gaaacagaaa cggaacttgc aactcgtttt
     7261 aacacctccc gatataaagt ccgtcaggca cttagtactc tcgtccaaat gggaatgctt
     7321 gaacgagccc ctcgcagagg aagtctgtta aaaaacgttg gccagagtag tattaacgac
     7381 caattgttga tgcagtttga tctatctggg tttgatatct cggaatttac agaggccaga
     7441 attttagttg agtgcgcgat cattccactg gcaacacgcc gaattttccc agcgatgctc
     7501 tccaaacttg aaaatgaatt gagattgatt gaggaaaatg cagacaatcc acaaatagca
     7561 gatcaacatg atcgggattt tcacctgctc ttgcttcagg ccagtggtaa tcgtgttctg
     7621 caggttttct ctgatgtgtt aatcgcttat tttgagaaaa caagagagtc gttaccggat
     7681 aatgattgtc aatacttcct tgatactgct tcaaaacaga gagagatatt gtcgtatatc
     7741 aaaaaaggcg atgcccaaat ggcttcagag ttaatgcgag ttcatttact ggaaaagaaa
     7801 atatagcatt aaatctttca aaaactaaaa ctcaaccgga aaacttatct ggtgtgagtg
     7861 ctatgtgata tcactgtcaa gttattagct gtgtttatcc taataaggta tctttgatat
     7921 ataagcgagc tttattggta aagagagcgc tatcatagca gactatgtta agttagagca
     7981 gggatgcgta agtctgagtg ccacgtcttc tgtacgtatc gacgccttat acgatctgga
     8041 aaatattatc aggcataaac ataaaggaga acttgcctga gtagttgcct tttgagaaat
     8101 ttatcttcac tggatgggag ttacctgcca acatgtgttc ccgtccaagg acagaaccaa
     8161 cagttgggtg ttgattacct ggtgacgcgg aaccaacaga agttgtaatt atgcaggtct
     8221 gaggagatca gtatcagaga tatccgaatg aactgtgata atggtttgac aaaaacggat
     8281 aacaatacaa cagaacacgt tcttagtaat ctcagccatg gaaataaaaa ttagatgtat
     8341 ctccaatcag tgagttgcgt ggaaattaca aggttctgct gtacgatctg agtgggttaa
     8401 cttgatattt tgtactcgtc acagaaaata tacagagata tacactctgg tgtaatcact
     8461 gttgtaggtt gatcaggtgt cactcttttt actataatgt ttagtataag caatgttttt
     8521 tcaaaaaacg ttcttagaag tagagttact aactccaaag ttaatgacac agaaccaatg
     8581 ataaaaaaaa ctaatccatt tccctcttaa aatttttaac aaaaataatt tcacatttta
     8641 ttttaacggg gaaatattaa atagtatatc taacccaggg aaagttaatt aaattccata
     8701 agtaacaata gggttgattt ttaaatcact atatagaata tttatataga tttaggtatt
     8761 acgttggcaa gctaggttgg aaaaatgaac aatgtattaa ttgttgatga cgatatatta
     8821 gtagctgatg tgattcgtcg cattgtagaa caaaccccgg catttaaatg ctgtggcata
     8881 gcgttatcac ttggtcaggc gaaagaaatt atatctgtaa ataaaaaact ggctgacttg
     8941 attttgcttg atctttatat caataaagat aacggacttg atttactccc ttttcttcat
     9001 agcattcatt gcaaaagtga tgttattgta atttctgctg ctgatgactc tgcgacaatt
     9061 aaagattcat tacattatgg tgtttctgat tatctgataa agccatttca tatctcacgt
     9121 ttggttgatt cgcttacacg atggaaacat aaaaagactc ttcttgagag tcagaagata
     9181 ttcaatcaat cagaactgga tttattaact cacaaaataa ttccgaacac agaaaaaata
     9241 agacatttac ccaaaggact gtcaacagat acattgcgaa ttatttgtag gtggattgat
     9301 gagcatcaac ataaaaaatt cacactcaat gaactggcca gtggtattaa gatatccaga
     9361 atctcatgca gaaaatatat catctggctg gtaagttgcc atatattggc atttgatttt
     9421 tcctgtggtg tcactggtcg ccctgcttat cgttactgga taagacatca atattaccca
     9481 tatattaaaa aatatagtga aaaaccgtaa gccttttaat catttatcta ccataattat
     9541 gacatctact taatcacaat aaactcatcg tgcgataatt tctggagagt gtgttccgga
     9601 tattaagagg ataaacgtat ggtcagggaa tagttatgtg cggtaaatat ggaagatttc
     9661 agattttttg acatttacac tgatgcatgg cactccccca ttgttcagaa agagtatatt
     9721 ctcccggatg cttcgttacc aatcctgccc gttttttccg caattttatc gtgaatgaat
     9781 ttgtcctgga acaacattgg tctaccagtg aacagaggat attatttctc tggattcaac
     9841 tgtgaattcg ctcatctatc tgccaagtaa tactccacgg tttttgataa gcacatttta
     9901 tgaatgttac tcatcatcac gatgagcatc aggatatcat taacaagccc ctcccgataa
     9961 atccggaatt aaccacattt catgggtaca agaaaaccat tatgtattga aatggtcccc
    10021 attattcatg tctgtcactt tattcattaa actgcctaaa accaatacta taaaagcccc
    10081 ggcacgttac cggggcgagg ttattagtac tgattgaata tctgctggat ctgctgcgga
    10141 tctttggttt gcgtcagagc cagttgcagc agaacacgtg ctttttgcgg gttcaacgtg
    10201 ccagaggcga cgaagccata tttcgcatca tccacttcgg catcctgagt ggttgctccc
    10261 gtcggtacgc gggaagaacg cactaccgca gtgccgtttt tcgcggcggt cgccagggtg
    10321 tcaaacacgg att
//
LOCUS       DAMZFT010000167        10227 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.167, whole genome shotgun sequence.
ACCESSION   DAMZFT010000167 DAMZFT010000000
VERSION     DAMZFT010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10227)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10227)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10227
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.167"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(<1..812)
                     /gene="ycjM"
                     /locus_tag="PN977_004421"
     CDS             complement(<1..812)
                     /gene="ycjM"
                     /locus_tag="PN977_004421"
                     /EC_number="2.4.1.352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosylglycerate phosphorylase"
                     /protein_id="HDI6044375.1"
                     /translation="MKQKITDYLDEIYGGTFTATHLQKLVTRLESAKRLITLRRKKHW
                     DESDVVLITYADQFHSNDLKPLPTFNQFYPQWLQSIFSHVHLLPFYPWSSDDGFSVID
                     YQQVASEAGEWQDIQQLGECSHLMFDFVCNHMSAKSEWFKNYLQQQPGFEDFFIAVDP
                     QTDLSVVTRPRALPLLTPFQMRDHSTRHLWTTFSDDQIDLNYRSPEVLLAMVDVLLCY
                     LEKGAEYVRLDAVGFMWKEPGTSCIHLEKTHLIIKLLRSIIDDVAPGTVIIT"
     gene            complement(1026..1340)
                     /gene="pspE"
                     /locus_tag="PN977_004422"
     CDS             complement(1026..1340)
                     /gene="pspE"
                     /locus_tag="PN977_004422"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase PspE"
                     /protein_id="HDI6044376.1"
                     /translation="MFKKGLLALALVFSLPVFAAEHWIDVRVPEQYQQEHVQGAINIP
                     LKEVKERIATAVPDKNDTVKVYCNAGRQSGQAKEILSEMGYTHVENAGGLKDIAMPKV
                     KG"
     gene            complement(1415..1636)
                     /gene="pspD"
                     /locus_tag="PN977_004423"
     CDS             complement(1415..1636)
                     /gene="pspD"
                     /locus_tag="PN977_004423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspD"
                     /protein_id="HDI6044377.1"
                     /translation="MNTRWQQAGQKVKPGFKLAGKLVLLTALRYGPAGVAGWAIKSVA
                     RRPLKMLLAVALEPLLSRAANKLAQRYKR"
     gene            complement(1645..2004)
                     /gene="pspC"
                     /locus_tag="PN977_004424"
     CDS             complement(1645..2004)
                     /gene="pspC"
                     /locus_tag="PN977_004424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspC"
                     /protein_id="HDI6044378.1"
                     /translation="MAGINLNKKLWRIPQQGMVRGVCAGIANYFDVPVKLVRILVVLS
                     IFFGLALFTLVAYIILSFALDPMPDNMAFGEQLPSSSELLDEVDRELAASETRLREME
                     RYVTSDTFTLRSRFRQL"
     gene            complement(2004..2228)
                     /gene="pspB"
                     /locus_tag="PN977_004425"
     CDS             complement(2004..2228)
                     /gene="pspB"
                     /locus_tag="PN977_004425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspB"
                     /protein_id="HDI6044379.1"
                     /translation="MSALFLAIPLTIFVLFVLPIWLWLHYSNRSGRSELSQSEQQRLA
                     QLADEAKRMRERIQALESILDAEHPNWRDR"
     gene            complement(2282..2950)
                     /gene="pspA"
                     /locus_tag="PN977_004426"
     CDS             complement(2282..2950)
                     /gene="pspA"
                     /locus_tag="PN977_004426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspA"
                     /protein_id="HDI6044380.1"
                     /translation="MGIFSRFADIVNANINALLEKAEDPQKLVRLMIQEMEDTLVEVR
                     STSARALAEKKQLTRRIEQASAREIEWQEKAELALLKEREDLARAALIEKQKLTDLIK
                     SLEHEVTLVDDTLVRMKKEIGELENKLSETRARQQALMLRHQAANSSRDVRRQLDSGK
                     LDEAMARFESFERRIDQMEAEAESHSFGKQKSLDDQFAELKADDAISEQLAQLKAKMK
                     QDNQ"
     gene            3117..4094
                     /gene="pspF"
                     /locus_tag="PN977_004427"
     CDS             3117..4094
                     /gene="pspF"
                     /locus_tag="PN977_004427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein operon transcriptional
                     activator"
                     /protein_id="HDI6044381.1"
                     /translation="MAEYKDNLLGEANSFLEVLEQVSHLAPLDKPVLIIGERGTGKEL
                     IASRLHYLSSRWQGPFISLNCAALNENLLDSELFGHEAGAFTGAQKRHPGRFERADGG
                     TLFLDELATAPMMVQEKLLRVIEYGELERVGGSQPLQVNVRLVCATNADLPAMVNEGT
                     FRADLLDRLAFDVVQLPPLRERESDIMLMAEHFAIQMCREIKLPLFPGFTERARETLL
                     NYRWPGNIRELKNVVERSVYRHGTSDYPLDDIIIDPFKRRPPEDAIAVSETTSLPTLP
                     LDLREFQMQQEKELLQLSLQQGKYNQKRAAELLGLTYHQFRALLKKHQI"
     gene            complement(4225..5490)
                     /gene="puuE"
                     /locus_tag="PN977_004428"
     CDS             complement(4225..5490)
                     /gene="puuE"
                     /locus_tag="PN977_004428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate transaminase"
                     /protein_id="HDI6044382.1"
                     /translation="MSNNEFHQRRLSATPRGVGVMCNFFAQSAENAMLKDVEGNEYID
                     FAAGIAVLNTGHRHPDLVAAVEQQLQQFTHTAYQIVPYESYVTLAEKINALAPVSGQA
                     KTAFFTTGAEAVENAVKIARAHTGRPGVIAFSGGFHGRTYMTMALTGKVAPYKIGFGP
                     FPGSVYHVPYPSDLHGVSTQDSLDAIERLFKSDIEAKQVAAIIFEPVQGEGGFNVAPK
                     ELVAAIRRLCDEHGIVMIADEVQSGFARTGKLFAMDHYADKPDLMTMAKSLAGGMPLS
                     GVVGNANIMDAPAPGGLGGTYAGNPLAVAAAHAVLNIIDKESLCERANQLGQRLTNTL
                     IDAKESVPAIAAVRGLGSMIAAEFNDPQTGEPSAAIAQKIQQRALAQGLLLLTCGAYG
                     NVIRFLYPLTIPDAQFDAAMKILQDALKD"
     gene            complement(5528..6808)
                     /gene="puuB"
                     /locus_tag="PN977_004429"
     CDS             complement(5528..6808)
                     /gene="puuB"
                     /locus_tag="PN977_004429"
                     /EC_number="1.4.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylputrescine oxidase"
                     /protein_id="HDI6044383.1"
                     /translation="MTEHTSSYYAASANKYAPFDTLNESISCDVCVVGGGYTGLSSAL
                     HLAEAGFDVVVLEASRIGFGASGRNGGQLVNSYSRDIDVIEKSYGMDTARMLGSMMFE
                     GGEIIRERIKRYQIDCDYRPGGLFVAMNDKQLATLEEQKENWERYGNKQLELLDANAI
                     RREVASDRYTGALLDHSGGHIHPLNLAIGEADAIRLNGGRVYELSAVTQIQHTTPAVV
                     RTAKGQVTAKYVIVAGNAYLGDKVEPELAKRSMPCGTQVITTERLSEDLARSLIPKNY
                     CVEDCNYLLDYYRLTADNRLLYGGGVVYGARDPDDVERLVVPKLLKTFPQLKGVKIDY
                     RWTGNFLLTLSRMPQFGRLDTNIYYMQGYSGHGVTCTHLAGRLIAELLRGDAERFDAF
                     ANLPHYPFPGGRTLRVPFTAMGAAYYSLRDRLGV"
     gene            complement(6810..8297)
                     /gene="puuC"
                     /locus_tag="PN977_004430"
     CDS             complement(6810..8297)
                     /gene="puuC"
                     /locus_tag="PN977_004430"
                     /EC_number="1.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase PuuC"
                     /protein_id="HDI6044384.1"
                     /translation="MNFHHLAYWQDKALSLAIETRLFINGEYTAAAENETFETVDPVT
                     QAPLAKIARGKSVDIDRAVSAARGVFERGDWSLSSPAKRKAVLNKLADLMEANAEELA
                     LLETLDTGKPIRHSLRDDIPGAARAIRWYAEAIDKVYGEVATTSSHELAMIVREPVGV
                     IAAIVPWNFPLLLTCWKLGPALAAGNSVILKPSEKSPLSAIRLAGLAKEAGLPDGVLN
                     VVTGFGHEAGQALSRHNDIDAIAFTGSTRTGKQLLKDAGDSNMKRVWLEAGGKSANIV
                     FADCPDLQQAASATAAGIFYNQGQVCIAGTRLLLEESIADEFLALLKQQAQNWQPGHP
                     LDPATTMGTLIDCAHADSVHSFIREGESKGQLLLDGRNAELAAAIGPTIFVEVDPNAS
                     LSREEIFGPVLVVTRFTSEEQALQLANDCQYGLGAAVWTRDLSRAHRMSRRLKAGSVF
                     VNNYNDGDMTVPFGGYKQSGNGRDKSLHALDKFTELKTIWISLEA"
     gene            complement(8447..9004)
                     /gene="puuR"
                     /locus_tag="PN977_004431"
     CDS             complement(8447..9004)
                     /gene="puuR"
                     /locus_tag="PN977_004431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001464354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator PuuR"
                     /protein_id="HDI6044385.1"
                     /translation="MSDEGLAPGKRLSEIRQQQGLSQRRAAELSGLTHSAISTIEQDK
                     VSPAISTLQKLLKVYGLSLSEFFSEPEKPDEPQVVINQDDLIEMGSQGVSMKLVHNGN
                     PNRTLAMIFETYQPGTTTGERIKHQGEEIGTVLEGEIVLTINGQDYHLVAGQSYAINT
                     GIPHSFSNTSAGICRIISAHTPTTF"
     gene            complement(9031..9795)
                     /gene="puuD"
                     /locus_tag="PN977_004432"
     CDS             complement(9031..9795)
                     /gene="puuD"
                     /locus_tag="PN977_004432"
                     /EC_number="3.5.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415814.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase"
                     /protein_id="HDI6044386.1"
                     /translation="MENIMNNPVIGVVMCRNRLKGHATQTLQEKYLNAIIHAGGLPIA
                     LPHALAEPSLLEQLLPKLDGIYLPGSPSNVQPHLYGENGDEPDADPGRDLLSMALINA
                     ALERRIPIFAICRGLQELVVATGGSLHRKLCEQPELLEHREDPELPVEQQYAPSHEVQ
                     VEEGGLLSALLPECSNFWVNSLHGQGAKVVSPRLRVEARSPDGLVEAVSVINHPFALG
                     VQWHPEWNSSEYALSRILFEGFITACQHHIAEKQRL"
     gene            10007..>10227
                     /locus_tag="PN977_004433"
     CDS             10007..>10227
                     /locus_tag="PN977_004433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase"
                     /protein_id="HDI6044387.1"
                     /translation="METNIVEVENFVQQSEERRGSAFTQEVKRYLERYPNTQYVDVLL
                     TDLNGCFRGKRIPVSSLKKLEKGCYFPASV"
BASE COUNT         2347 a         2742 c         2596 g         2542 t
ORIGIN      
        1 tcggtaatga tcactgtacc tggtgctacg tcatcaataa tcgaccgtaa cagtttgata
       61 atcagatgtg ttttttccag atggatgcag cttgttcccg gctctttcca cataaagcca
      121 acggcatcca gacggacata ttctgcgccc ttttccaggt agcacagcaa tacatccacc
      181 atcgccagta acacttcagg gctacggtaa ttcaggtcaa tttgatcgtc actaaaggtg
      241 gtccataaat ggcgcgttga atgatcgcgc atctggaatg gcgttaataa cggtaacgca
      301 cgcgggcgag tgacgacgct gagatcggtt tgcgggtcaa cggcaataaa aaaatcttca
      361 aaacctggct gctgttgtaa atagttttta aaccattcac tttttgccga catatggttg
      421 cagacaaaat caaacattaa atgactgcat tcaccgagtt gctgaatatc ctgccactcc
      481 cccgcttcac tagcgacctg ttgataatca attaccgaaa agccatcatc agatgaccat
      541 ggataaaaag gtaataaatg aacatgtgaa aaaatgcttt gcagccattg agggtaaaac
      601 tgattaaatg tgggtagtgg ttttaaatca ttactgtgaa attgatcggc ataggtgatt
      661 aacacgacat cactttcatc ccagtgtttt ttacgtcgca gtgtaattaa tcgtttcgca
      721 ctctcaagac gcgttacaag tttctgtaaa tgagttgcgg taaatgttcc accgtagatt
      781 tcgtccaggt aatccgtaat tttctgtttc atgttgcctt tccgtggtag cggtcccatg
      841 aaacgagaga ctactccgca gaaaataaac tgtcaattga gcacaagggt tttagtcacg
      901 caggaaatca ttttttacca gggaaaaagc gtatttgtga gctgacgcaa ataatctatg
      961 gggatgtaaa taaggtgtaa cagaaaaagt aaacggcgca taagcgccgc ttagggtgga
     1021 ttcttttaac ctttgacctt cggcattgcg atgtctttca ggccgccggc gttctcaacg
     1081 tgggtatatc ccatctcgct aaggatctct tttgcctgcc ctgactggcg tccggcattg
     1141 caatacactt tcacggtgtc gtttttatcc ggaacggcgg tggcaatgcg ctctttcact
     1201 tccttcaggg gaatattgat ggccccctga acgtgctctt gctgatactg ctcaggaaca
     1261 cgaacatcga tccagtgttc agcggcgaaa acgggcagtg aaaacaccag tgccagagca
     1321 agtaagcctt ttttaaacat gtaagcgtcc ttctatggat gaaaattgcc atccattata
     1381 cgcctgagat ttaacaattt ttgcgaaatt cccctcacct tttataacgc tgtgccagtt
     1441 tattggcagc ccgacttaac agcggttcca gcgccacagc cagcaacatt ttcagcggtc
     1501 ggcgagcaac tgattttatc gcccagcccg ccacacccgc cgggccatag cgcagtgcgg
     1561 taagaagtac cagcttgcct gctaatttga acccaggctt caccttttgc ccggcctgtt
     1621 gccagcgagt attcataact ttcctcacag ttgacggaaa cggctacgta acgtgaaagt
     1681 atcggaagtg acataacgtt ccatctcgcg taaacgcgtt tcacttgccg ccagttcacg
     1741 gtcgacttca tccagcaatt cgctgctgga aggtagctgc tcaccaaagg ccatgttgtc
     1801 cggcattgga tcaagcgcaa atgacaaaat gatgtaagca accagggtaa acagcgccag
     1861 accgaagaaa atcgacagca ccaccaggat acgcaccagt tttactggta catcaaaata
     1921 gttggcaatc ccggcgcaga cgccacggac catgccctgc tgtggaatac gccataattt
     1981 tttattgaga ttaatgcccg ccattagcga tccctccagt tcggatgttc tgcatcaaga
     2041 atggattcca gcgcctgaat acgttcgcgc atccgttttg cttcatcagc cagttgcgct
     2101 aatcgctgct gctcactttg cgacaattca ctgcgaccag aacgattgct gtaatgcagc
     2161 cataaccaga tcggtaaaac aaacagcaca aaaatggtta acggaatagc cagaaatagc
     2221 gcgctcataa ggactcctta gacgatgagc ggcggcgcgt tcggacgccg cctggatgtt
     2281 attattgatt gtcttgcttc attttggctt ttaattgtgc cagttgctcg ctgattgcat
     2341 catcggcttt cagttcggca aactgatcgt ccagcgattt ttgtttaccg aagctgtggc
     2401 tttctgcttc cgcttccatc tggtcgatac ggcgttcgaa agattcgaaa cgagccattg
     2461 cttcatccag tttgccactg tccagctgac gacgcacatc gcgcgacgag tttgccgcct
     2521 gatggcgtaa catcaatgcc tgctggcgag cgcgtgtttc gctcaatttg ttttccagct
     2581 cgccgatctc tttcttcata cgcaccagcg tatcgtccac cagcgtcact tcatgttcca
     2641 ggctcttaat cagatcggtc agtttctgtt tttcaattaa cgccgcacgc gccagatcct
     2701 ctctctcttt cagcagtgcc agttccgctt tttcctgcca ttcaatttca cgagccgacg
     2761 cttgttcaat acggcgagtc agctgtttct tttctgccag cgcacgcgcc gaagtagaac
     2821 gtacttcaac caatgtatct tccatctcct ggatcatcag acgcaccagt ttctgtggat
     2881 cttccgcttt ctctaacaga gcgttgatgt tggcattcac gatgtcggca aagcgagaaa
     2941 aaatacccat aatgttgtcc tcttgatttc tgcattcagg attgtcctgc tgaactatta
     3001 atacaatttg cgtgccaatt ttttatcttt ttgatttata aagatctgat tgaagaatca
     3061 acagcaacat gccaggatga attagcgaat tacactaaca agtggcgaat ttcatcatgg
     3121 cagaatacaa agataattta cttggtgagg cgaatagctt tctcgaagtg ctggaacagg
     3181 tttcgcatct cgcaccactg gataaaccgg tgctcatcat cggcgaacgc ggcaccggta
     3241 aagagttgat tgccagccgc ctgcattatc tctcctcccg ttggcaaggg ccgtttattt
     3301 cccttaactg cgcggcgtta aatgaaaatc tgctggattc cgaactgttt ggtcacgaag
     3361 cgggggcgtt taccggtgcg caaaaacgtc atcctgggag atttgaacgt gccgatggcg
     3421 gtacgctatt tcttgatgaa ctcgctacgg caccgatgat ggtgcaggaa aaattattgc
     3481 gtgtgattga gtacggtgaa ctggagcgcg ttggcggtag tcagccattg caggtgaatg
     3541 tgcggttggt atgcgcgacg aatgccgatc tcccggcgat ggtcaatgaa ggtacttttc
     3601 gcgctgacct gctcgaccgg ctggctttcg atgtagtgca actgccgccc ctgcgcgagc
     3661 gcgaaagcga cattatgctg atggcagaac actttgccat tcagatgtgt cgggaaatca
     3721 agctgcctct gttcccgggt tttacggagc gcgccagaga aacattgctg aattatcgct
     3781 ggccgggaaa tattcgtgaa ttgaaaaacg tggtggaacg ttcagtgtat cgccacggca
     3841 ccagcgatta tccgcttgat gacatcatta ttgatccctt taaacggcgt ccgcctgaag
     3901 acgctatcgc cgtttcagaa accacctcgc ttccaacact gccgctggat ttacgtgagt
     3961 ttcagatgca gcaggaaaaa gagttgctgc aactcagttt gcaacagggg aaatataacc
     4021 agaagcgcgc ggctgaatta ctggggttaa cctatcatca gttccgcgcg ttgttgaaaa
     4081 agcaccagat ttagcgaagt tgggcaatac aacttgccgg atgcggcgtg aacgccttat
     4141 ccggcataca taatcaatac gtcgtttgca ggcctgataa gcgcagcgta tcagacatta
     4201 ttgcgttgac cctgcttctg cgtattaatc tttcagcgca tcctgcaaaa ttttcattgc
     4261 cgcatcgaat tgcgcatccg ggatggtcag cggatacagg aaacgaatca cgttgccgta
     4321 tgcgccacag gtcagcagaa gcagcccctg cgccagcgcg cgttgctgga ttttctgtgc
     4381 aatcgccgct gacggctcgc ccgtttgcgg atcgttaaac tctgccgcaa tcatagaccc
     4441 cagaccgcgt accgccgcga tggccggaac gctttctttg gcatcaatca acgtgtttgt
     4501 caggcgctgg cccagttgat tcgcgcgttc acagagtgat tctttgtcga taatgttgag
     4561 cacagcgtgc gcggcagcca ccgccagcgg gttcccggcg taagtaccac ccaacccgcc
     4621 cggcgcgggc gcgtccataa tattcgcgtt accgaccacg cccgaaagcg gcatgccgcc
     4681 cgcgaggctt ttcgccatcg tcattaagtc cggcttatcg gcgtagtgat ccatggcaaa
     4741 cagtttaccg gtacgcgcaa agccgctttg cacttcatcg gcaatcatca caatgccgtg
     4801 ctcgtcgcac aggcggcgaa tggcggcaac cagctctttt ggtgcaacgt tgaaaccgcc
     4861 ttcgccctgc accggttcga aaataatcgc cgccacctgc ttcgcttcaa tgtcagattt
     4921 aaacaagcgt tcgatggcgt cgagagagtc ctgcgttgaa acgccatgta aatctgacgg
     4981 ataaggtacg tgatataccg aaccggggaa cgggccgaag ccgattttgt acggcgcgac
     5041 ctttccggtc agcgccatgg tcatatatgt acgaccgtgg aagccgccgc taaacgcaat
     5101 cacgccaggg cgtccggtat gggcgcgggc gattttcacc gcgttctcca ccgcttccgc
     5161 accggtggtg aagaacgcag ttttagcctg cccgctcacc ggggcaaggg cgttgatttt
     5221 ctccgccagg gtgacgtagc tttcgtacgg cacaatctga tacgccgtgt gggtaaactg
     5281 ttgcaattgc tgctccaccg ccgcgaccag atcagggtgg cgatgcccgg tattcagcac
     5341 cgcaatgcct gcggcgaaat cgatgtattc gttgccctct acgtccttca gcatggcgtt
     5401 ttctgccgac tgggcgaaga agttacacat cacgccaacc ccgcgagggg tagcagaaag
     5461 acgacgctga tggaattcat tgttgctcat gtttcacacc ttttgactct tcactgttaa
     5521 tcggaaatta aacgcccaga cgatcgcgca ggctgtaata agccgcgccc atcgcggtaa
     5581 atggcacgcg cagcgtgcgc ccgccgggga acgggtaatg cggcagattg gcaaaggcgt
     5641 cgaagcgttc ggcgtcaccg cgcagcagtt cagcaatcaa acgcccggca agatgggtac
     5701 aggtgacgcc gtggccgctg taaccctgca tgtaatagat gttggtatca aggcgaccga
     5761 actgcggcat gcgtgacagg gtcagcagga agttgcccgt ccagcggtaa tcaattttca
     5821 cgcccttcag ctgcgggaag gttttcagca gtttcggcac cacaaggcgc tcaacatcgt
     5881 ccgggtcgcg tgcgccgtag accacgccgc cgccgtacag caggcggtta tcggcagtca
     5941 ggcggtagta atcaagaaga tagttacagt cttccacaca gtagtttttc gggatcagcg
     6001 aacgggctaa atcttccgac agccgttcgg tggtgatcac ctgcgtgccg cacggcatgc
     6061 tgcgtttcgc tagttccggc tctactttat cgcccagata cgcattcccg gcgacaatca
     6121 cgtatttcgc cgtcacctga cctttggcag ttcgcacaac ggctggcgtg gtgtgctgga
     6181 tctgcgtcac ggcagaaagt tcatacacgc gcccgccgtt caggcggatg gcgtccgctt
     6241 cgccaatggc gaggtttagc ggatgaatat gcccaccgct gtgatccagc agcgcaccag
     6301 tgtagcgatc gctggcgacc tcgcggcgaa tagcgttggc gtccagcagc tccagctgtt
     6361 tattgccgta gcgttcccag ttctctttct gctcttcgag tgtggcgagc tgtttatcat
     6421 tcatcgccac aaacaggccg ccggggcggt agtcgcagtc aatctgataa cgtttgatac
     6481 gttcgcggat gatctctccg ccttcaaaca tcatgctgcc gagcatacgg gcggtgtcca
     6541 tgccgtagct tttttcgatc acgtcgatgt cgcggctgta ggagttcaca agctgcccgc
     6601 cattgcgtcc gcttgcgccg aagccgatgc gtgaggcttc gagaaccact acatcaaagc
     6661 ccgcttccgc cagatgcagc gcggaggaaa gcccggtata gccgccaccg accacgcaaa
     6721 cgtcgcagga aatcgactca ttcagcgtgt cgaatggcgc gtatttattc gcactggcgg
     6781 cgtagtaact gctggtatgt tcggtcattt caggcctcca ggcttatcca gatggttttc
     6841 agttcggtga atttgtcaag ggcatgcagg gatttgtcgc gcccgttgcc gctctgctta
     6901 tagccgccaa acggcacggt catatcgccg tcgttgtagt tattgacgaa gacggagccg
     6961 gctttcaggc ggcgactcat gcggtgcgcg cgggagaggt cgcgcgtcca taccgccgcg
     7021 ccaagtccgt actggcagtc gttggcaagc tgtagcgcct gttcttctga tgtgaaacgc
     7081 gtgaccacca gcaccggacc gaaaatctct tcgcggctta aggacgcatt cgggtctacc
     7141 tccacaaaga tggtcgggcc gatggcggca gccagctcgg cgttacggcc atccagcagc
     7201 agttgccctt tgctttcgcc ttcccgaata aagctatgga ccgagtcggc gtgggcgcag
     7261 tcgattaagg tgcccatggt ggttgcggga tcaagtggat ggcccggctg ccagttttgc
     7321 gcctgctgtt ttaacagggc taagaattca tcggcgatgc tctcttccag caacaaacgc
     7381 gttccggcga tgcacacctg gccctggttg tagaaaatgc ctgcggcggt ggcgcttgct
     7441 gcctgttgta aatccgggca gtcggcgaaa acgatgttgg cgcttttgcc gcccgcctcc
     7501 agccagacgc gtttcatgtt gctgtcgccc gcgtccttca gcagctgttt cccggtgcgg
     7561 gttgagccag taaaggcaat ggcatcgata tcgttatggc gcgaaagcgc ctgcccggct
     7621 tcatgaccaa aacccgtcac cacgttcaac acaccatccg gcagccccgc ttctttcgcc
     7681 agccccgcga gacgaatcgc actgagcggt gatttttcag acggttttag aatcacgctg
     7741 tttcccgccg ccagcgccgg gccgagtttc cagcaagtca gcagcagcgg gaagttccac
     7801 ggcacgatgg cggcaatcac gccgaccggt tcacgcacga tcatcgccag ctcatggcta
     7861 ctggtggtcg ccacttcgcc atacactttg tcgatcgctt cggcgtacca gcgaatggcg
     7921 cgcgccgcgc cgggaatatc atcacgcagg ctgtgacgaa tcggtttgcc ggtgtcgagc
     7981 gtttccagca gcgccagttc ttcagcgtta gcttccatta aatcggcgag tttattcagt
     8041 accgctttac gtttagccgg agaagagagt gaccagtcgc cgcgttcaaa tacgccgcgt
     8101 gctgcgctca ccgcacggtc gatatcgacg ctcttgccgc gggcaatttt cgccagcggt
     8161 gcctgggtga ccggatcaac ggtttcaaaa gtttcatttt ccgccgcagc agtatattca
     8221 ccgttaataa ataagcgggt ttcaatggcg agacttaacg ctttatcctg ccagtaagcc
     8281 agatgatgaa aattcattat gactcctgtt tcacgtctat cagatatatg cagtttgtga
     8341 acgtcagggc gcagagatcc ccgggccgta tgcccgttta tttctggcga cattctttat
     8401 ggattgccgg gcgcattgcg cttacccggc ttacagaaca aaaagattaa aacgtggtgg
     8461 gcgtatgggc gctgataatt cggcaaatac ctgccgacgt attactgaaa ctgtgcggga
     8521 tgccggtatt aatggcataa ctttgccccg cgacgagatg ataatcctga ccattaatcg
     8581 tcagaacaat ttcaccttcc agtacagtgc ctatttcctc accctgatgc ttaattcttt
     8641 ccccagtggt tgtgcccggc tggtacgttt caaagatcat cgccagcgtg cgattcgggt
     8701 taccgttatg aaccagcttc attgacacac cctgactgcc catctcaatt aagtcgtcct
     8761 gattaatgac gacctgcggc tcatcaggtt tttccggctc ggaaaagaat tccgagagtg
     8821 acagaccata caccttcagc agcttttgca gcgtactgat ggcagggctg actttatctt
     8881 gttctatcgt actgatagca ctgtgagtca gcccggagag ttcggcggca cgacgttgtg
     8941 aaagcccctg ctgctggcgg atttccgaca agcgttttcc tggcgccagt ccctcatcac
     9001 tcatatttgc atttccttaa actgtagtgg tcagagtcgc tgtttttcag cgatatggtg
     9061 ctgacaagcg gtgataaagc cctcgaacaa tatacgcgaa agcgcgtact cgctactgtt
     9121 ccactccggg tgccactgta cgcccagcgc aaaaggatga ttgatgacgc taaccgcctc
     9181 aactaagcca tccggcgagc gagcttcaac acgcaaccgt gggctaacga ccttcgcccc
     9241 ttgtccatgc agagagttta cccaaaagtt gctacattca ggtaacaacg cagacagcaa
     9301 tcccccctct tcaacctgaa cttcatgcga cggtgcatat tgctgctcca ccggcagttc
     9361 gggatcttcc cgatgttcca gcaattcagg ctgttcgcac agcttgcgat gcaacgaacc
     9421 accggttgcc accaccagtt cctgtaaacc ccggcagatg gcgaaaatgg ggatgcgcct
     9481 ttcgagtgcg gcattaatta acgccatgct cagaagatca cgcccgggat cggcgtcagg
     9541 ctcatcgccg ttttcaccat ataggtgcgg ctgcacattg ctgggactac caggaagata
     9601 aataccatcg agtttcggca aaagttgttc aagtaacgac ggttccgcca gcgcatgtgg
     9661 tagcgctatt ggcaaaccgc ctgcgtggat gatggcattc aggtactttt cttgcagagt
     9721 ctgggtcgca tgacccttaa gcctgttcct gcacattacg acaccgataa ccggattgtt
     9781 cattatattt tccatgctca atctcacaaa gtggactaaa ttatcgccat tactgcgctt
     9841 taaatgacct ttatgttcaa tattttttca atctagcagt ggtttttcat ttttgcaaac
     9901 tcaatttaac atttgacaaa catttagttt gcatacagat tcgaatagtg gtcattatat
     9961 tttacgcttt gataacgagc ggaaaacaaa ccaaaggcga agaatcatgg aaaccaatat
    10021 cgttgaagta gagaactttg ttcagcagtc agaagagagg cggggtagcg cctttacgca
    10081 ggaagtgaaa cgctacctgg agcgctaccc gaatacgcaa tatgttgatg ttttacttac
    10141 cgatttgaat ggttgcttcc gtggaaaacg tattcctgtt tcaagcctga agaagctcga
    10201 gaaagggtgt tatttcccgg cctcggt
//
LOCUS       DAMZFT010000168        10122 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.168, whole genome shotgun sequence.
ACCESSION   DAMZFT010000168 DAMZFT010000000
VERSION     DAMZFT010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10122)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10122)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10122
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.168"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..512
                     /gene="argB"
                     /locus_tag="PN977_004434"
     CDS             <1..512
                     /gene="argB"
                     /locus_tag="PN977_004434"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418394.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HDI6044388.1"
                     /translation="AWAKKHQIAAVGLFLGDGDSVKVTQLDEELGHVGLAQPGSPKLI
                     NSLLENGYLPVVSSIGVTDEGQLMNVNADQAATALAATLGADLILLSDVSGILDGKGQ
                     RIAEMTAAKAEQLIEQGIITDGMIVKVNAALDAARTLGRPVDIASWRHAEQLPALFNG
                     MPMGTRILA"
     gene            573..1946
                     /gene="argH"
                     /locus_tag="PN977_004435"
     CDS             573..1946
                     /gene="argH"
                     /locus_tag="PN977_004435"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HDI6044389.1"
                     /translation="MALWGGRFTQAADQRFKQFNDSLRFDYRLAEQDIVGSVAWSKAL
                     VTVGVLTAEEQAQLEEALNVLLEDVRARPQQILESDAEDIHSWVEGKLIDKVGQLGKK
                     LHTGRSRNDQVATDLKLWCKDTVSELLTANRQLQSALVETAQNNQDAVMPGYTHLQRA
                     QPVTFAHWCLAYVEMLARDESRLQDALKRLDVSPLGCGALAGTAYEIDREQLAGWLGF
                     ASATRNSLDSVSDRDHVLELLSAAAIGMVHLSRFAEDLIFFNTGEAGFVELSDRVTSG
                     SSLMPQKKNPDALELIRGKCGRVQGALTGMMMTLKGLPLAYNKDMQEDKEGLFDALDT
                     WLDCLHMAALVLDGIQVKRPRCQEAAQQGYANATELADYLVAKGVPFREAHHIVGEAV
                     VEAIRQGKPLEELPLSELQKFSQVIGEDVYPILSLQSCLDKRAAKGGVSPQQVAQAIA
                     FAQARLG"
     gene            2213..3130
                     /gene="oxyR"
                     /locus_tag="PN977_004436"
     CDS             2213..3130
                     /gene="oxyR"
                     /locus_tag="PN977_004436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator OxyR"
                     /protein_id="HDI6044390.1"
                     /translation="MNIRDLEYLVALAEHRHFRRAADSCHVSQPTLSGQIRKLEDELG
                     VMLLERTSRKVLFTQAGMLLVDQARTVLREVKVLKEMASQQGETMSGPLHIGLIPTVG
                     PYLLPHIIPMLHQTFPKLEMYLHEAQTHQLLAQLDSGKLDCVILALVKESEAFIEVPL
                     FDEPMLLAIYEDHPWANRECVPMADLAGEKLLMLEDGHCLRDQAMGFCFEAGADEDTH
                     FRATSLETLRNMVAAGSGITLLPALAVPPERKRDGVVYLPCIKPEPRRTIGLVYRPGS
                     PLRSRYEQLAEAIRARMDGHFDKVLKQAV"
     gene            complement(3113..4513)
                     /gene="sthA"
                     /locus_tag="PN977_004437"
     CDS             complement(3113..4513)
                     /gene="sthA"
                     /locus_tag="PN977_004437"
                     /EC_number="1.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Si-specific NAD(P)(+) transhydrogenase"
                     /protein_id="HDI6044391.1"
                     /translation="MPHSYDYDAIVIGSGPGGEGAAMGLVKQGARVAVIERYQNVGGG
                     CTHWGTIPSKALRHAVSRIIEFNQNPLYSDHSRLLRSSFADILNHADNVINQQTRMRQ
                     GFYERNHCEILQGNARFVDEHTLALDCPDGSVETLTAEKFVIACGSRPYHPTDVDFTH
                     PRIYDSDSILSMHHEPRHVLIYGAGVIGCEYASIFRGMDVKVDLINTRDRLLAFLDQE
                     MSDSLSYHFWNSGVVIRHNEEYEKIEGCDDGVIMHLKSGKKLKADCLLYANGRTGNTD
                     SLALQNIGLETDSRGQLKVNSMYQTAQPHVYAVGDVIGYPSLASAAYDQGRIAAQALV
                     KGEANAHLIEDIPTGIYTIPEISSVGKTEQQLTAMKVPYEVGRAQFKHLARAQIVGMN
                     VGTLKILFHRETKEILGIHCFGERAAEIIHIGQAIMEQKGGGNTIEYFVNTTFNYPTM
                     AEAYRVAALNGLNRLF"
     gene            4845..5495
                     /gene="fabR"
                     /locus_tag="PN977_004438"
     CDS             4845..5495
                     /gene="fabR"
                     /locus_tag="PN977_004438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002460670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor FabR"
                     /protein_id="HDI6044392.1"
                     /translation="MMGVRAQQKEKTRRSLVEAAFSQLSAERSFASLSLREVAREAGI
                     APTSFYRHFRDVDELGLTMVDESGLMLRQLMRQARQRIAKGGSVIRTSVSTFMEFIGN
                     NPNAFRLLLRERSGTSAAFRAAVAREIQHFIAELADYLELENHMPRAFTEAQAEAMVT
                     IVFSAGAEALDVGVEQRRQLEERLVLQLRMISKGAYYWYRREQEKTAIIPGNVKDE"
     gene            5495..5854
                     /locus_tag="PN977_004439"
     CDS             5495..5854
                     /locus_tag="PN977_004439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YijD family membrane protein"
                     /protein_id="HDI6044393.1"
                     /translation="MKQANQDRGTLLLALVAGLSINGTFAALFSSIVPFSVFPIISLV
                     LTVYCLHQRYLNRTMPVGLPGLAAACFILGVLLYSTVVRAEYPDIGSNFFPAVLSVIM
                     VFWIGAKMRNRKQEVAE"
     gene            complement(5894..6994)
                     /gene="trmA"
                     /locus_tag="PN977_004440"
     CDS             complement(5894..6994)
                     /gene="trmA"
                     /locus_tag="PN977_004440"
                     /EC_number="2.1.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (uridine(54)-C5)-methyltransferase TrmA"
                     /protein_id="HDI6044394.1"
                     /translation="MTPEHLPTEQYEAQLAEKVVRLQSMMAPFSDLVPEVFRSPVSHY
                     RMRAEFRIWHDGDDLYHIIFDQQTKSRIRVDSFPAASELINQLMTAMIAGVRNNPVLR
                     HKLFQIDYLTTLSNQAVVSLLYHKKLDDEWRQEAEALRDALRAQNLNVHLIGRATKTK
                     IELDQDYIDERLPVAGKEMIYRQVENSFTQPNAAMNIQMLEWALDVTKGSKGDLLELY
                     CGNGNFSLALARNFDRVLATEIAKPSVAAAQYNIAANHIDNVQIIRMAAEEFTQAMNG
                     VREFNRLQGIDLKSYQCETIFVDPPRSGLDGETEKMVQAYPRILYISCNPETLCKNLE
                     TLSQTHKVERLALFDQFPYTHHMECGVLLTAK"
     regulatory      7108..7298
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            7363..9207
                     /gene="btuB"
                     /locus_tag="PN977_004441"
     CDS             7363..9207
                     /gene="btuB"
                     /locus_tag="PN977_004441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent vitamin B12 receptor BtuB"
                     /protein_id="HDI6044395.1"
                     /translation="MIKKASLLTACSVTAFSAWAQDTSPDTLVVTANRFEQPRSTVLA
                     PTTVVTRQDIDRWQSTSVNDVLRRLPGVDITQNGGSGQLSSIFIRGTNASHVLVLIDG
                     VRLNLAGVSGSADLSQFPIALVQRIEYIRGPRSAVYGSDAIGGVVNIITTRDEPGTEI
                     SAGWGSNSYQNYDVSTQQQLGDKTRVTLLGDYAHTHGYDVVAYGNTGTQAQTDNDGFL
                     SKTLYGALEHNFTDAWSGFVRGYGYDNRTNYDAYYSPGSPLLDTRKLYSQSWDAGLRY
                     NGELIKSQLITSYSHSKDYNYDPHYGRYDSSATLDEMKQYTVQWANNVTVGHGNIGAG
                     VDWQKQTTTPGTGYVEDGYDQRNTGIYLTGLQQVGDFTFEGAARSDDNSQFGRHGTWQ
                     TSAGWEFIEGYRFIASYGTSYKAPNLGQLYGFYGNPNLNPEKSKQWEGAFEGLTAGVN
                     WRISGYRNDVSDLIDYDDHTLKYYNEGKARIKGVEATANFDTGPLTHTVSYDYVDARN
                     AITDVPLLRRAKQQVKYQLDWQLYDFDWGITYQYLGTRYDKDYSSYPYQTVKMGGVSL
                     WDLAVAYPVTSHLTVRGKIANLFDKDYETVYGYQTAGREYTLSGSYTF"
     gene            9152..10009
                     /gene="murI"
                     /locus_tag="PN977_004442"
     CDS             9152..10009
                     /gene="murI"
                     /locus_tag="PN977_004442"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HDI6044396.1"
                     /translation="MATKLQDGNTPCLAATPSEPRPTVLVFDSGVGGLSVYDEIRHLL
                     PDLHYIYAFDNVAFPYGEKSEAFIVERVVAIVTAVQKRYPLALAVVACNTASTVSLPA
                     LREKFDFPVVGVVPAIKPAARLTANGIVGLLATRGTVKRSYTHELIARFANECQIEML
                     GSAEMVELAEAKLHGEDVSLDALKRILRPWLRMKEPPDTVVLGCTHFPLLQEELLQVL
                     PEGTRLVDSGAAIARRTAWLLEHEAPDAKSADANIAFCMAMTPEAEQLLPVLQRYGFE
                     TLEKLAVLG"
BASE COUNT         2342 a         2461 c         2783 g         2536 t
ORIGIN      
        1 tggcatgggc gaagaaacat cagattgcgg ccgtaggttt gtttctcggt gacggcgaca
       61 gcgtcaaagt gacccagctt gatgaagagt taggccatgt tggactggcg cagccaggtt
      121 cgcctaagct tatcaactcc ttgctggaga acggttatct gccggtggtc agctccattg
      181 gcgtaacaga cgaagggcaa ctgatgaacg tcaatgccga tcaggcggca acggcgctgg
      241 cggcaacgct gggcgcggat ctgattttgc tctccgacgt cagcggcatt ctcgacggca
      301 aagggcaacg tattgccgaa atgaccgccg cgaaagcaga acaactgatt gagcagggca
      361 ttattactga cggcatgata gtgaaagtga acgcggcgct ggatgctgcg cgcacgctgg
      421 gccgcccggt ggatatcgcc tcctggcgtc atgcggaaca gcttccggca ctgtttaacg
      481 gtatgccgat gggtacgcgg attttagctt aagttttgtt ggccggaggc gcagctttcc
      541 ggcattgaat ttcaaaataa ggaaacagag ttatggcact ttggggcggg cgttttaccc
      601 aggcagcaga tcaacggttc aaacaattca acgactcact gcgctttgat taccgtctgg
      661 cggagcagga tattgttggc tctgtggcct ggtccaaagc cctggtaacg gtcggcgtgt
      721 taaccgcaga agagcaggcg caactggaag aggcgctgaa cgtgctgctg gaagatgttc
      781 gcgccaggcc acaacaaatc cttgaaagcg acgccgaaga tatccatagc tgggtggaag
      841 gtaaactgat cgacaaagtg ggtcagttag gcaaaaaact gcataccggg cgtagccgta
      901 atgatcaggt agcaactgac ctgaaactgt ggtgcaaaga taccgttagc gagttgctga
      961 cggctaaccg gcagttacaa tccgctctgg tcgagaccgc gcagaacaat caggacgcgg
     1021 tgatgccggg ttacactcac ctgcaacgtg ctcaaccggt gacgttcgcg cactggtgcc
     1081 tggcctacgt tgagatgctg gcgcgtgatg aaagccgttt gcaggacgcg cttaagcgtc
     1141 tggatgtcag cccgctaggc tgtggcgcac tggcaggaac ggcctatgaa atcgaccgag
     1201 aacagttagc aggctggctg ggctttgctt cggcgacccg taacagtctc gacagcgttt
     1261 ctgaccgtga ccatgtgttg gaactgcttt ctgctgccgc tatcggcatg gtgcatctgt
     1321 cgcgttttgc tgaagatctg attttcttta acaccggcga agcggggttt gtcgagcttt
     1381 cggaccgcgt gacttccggt tcatcattaa tgccgcagaa gaaaaacccg gatgcgctgg
     1441 agctgattcg cggtaaatgc ggccgggtgc agggtgcgtt aaccggcatg atgatgacgc
     1501 tgaaaggttt gccgctggct tacaacaaag atatgcagga agacaaagaa ggtctgttcg
     1561 acgcgctcga tacctggctg gactgcctgc atatggcggc gctggtgctg gacggcattc
     1621 aggtgaaacg tccgcgttgc caggaagcgg cgcagcaggg gtatgcgaac gccactgaac
     1681 tggcggatta cctggtggcg aaaggcgtac cgttccgcga ggcgcatcat attgtgggtg
     1741 aagcggtggt ggaagccatt cgtcagggca aaccgctgga agaactaccg ctcagtgagt
     1801 tgcagaaatt cagtcaggtg attggcgaag atgtctatcc gattctgtcg ctacaatcgt
     1861 gcctcgacaa gcgtgcggca aaaggcggcg tctcaccgca gcaggtggcg caggcgattg
     1921 cttttgcgca ggctcggttg gggtaagaac atttatatgt attaatttga gcctggctta
     1981 tcgctgggct ttttatggca aaaaaaaagc ggatcctgga gatccgcaaa agttcacgtt
     2041 ggctttagtt attcgagttg agaaactctc gaaacgggca gtgacttcaa gggttaaaag
     2101 aggtgccgct ccgtttctgt gagcaattat cagtcagaat gcttgatagg gataatcgtt
     2161 cgttgctatg ctacctatcg ccgcgaacta tcgtggcaat ggaggatgga taatgaatat
     2221 tcgtgatctt gagtacctgg tggcattggc tgaacaccgc cattttcggc gtgcggcaga
     2281 ttcctgccac gttagccagc cgacgcttag cgggcaaatt cgtaagctgg aagatgagct
     2341 gggcgtgatg ttgctggagc ggaccagccg taaagtattg ttcacccagg cgggaatgct
     2401 gctggtggat caggcgcgta ccgtgctgcg tgaggtgaaa gtccttaaag agatggcaag
     2461 ccagcagggc gagacgatgt ccgggccgct gcatattggt ttgattccca cggttggacc
     2521 gtatctgcta ccgcatatta tcccgatgct gcaccagacc tttccaaagc tggaaatgta
     2581 tctgcatgaa gcacagaccc accagttact ggcgcaactg gacagcggca agctcgattg
     2641 cgtgatcctc gcgctggtga aagagagcga agcattcatt gaagtgccgt tgtttgatga
     2701 gccaatgttg ctggctatct atgaagatca cccgtgggcg aaccgcgaat gcgtaccgat
     2761 ggccgatctg gcaggagaaa aactgctgat gctggaagat ggtcactgtt tgcgcgatca
     2821 ggcaatgggg ttctgttttg aagccggggc ggatgaagat acacacttcc gcgcgaccag
     2881 cctggagacg ctgcgcaaca tggtggcggc aggtagcgga atcactttac tgcctgcgct
     2941 ggctgtgccg ccggaacgca aacgcgatgg ggttgtttat ctgccgtgca ttaagccgga
     3001 accacgccgc actattggcc tggtttatcg tcctggctca ccactgcgca gccgctatga
     3061 gcagctggca gaggccatcc gcgcaagaat ggatggccat ttcgataaag tgttaaaaca
     3121 ggcggtttaa gccatttagc gcagctaccc gataggcttc cgccatcgtc gggtagttaa
     3181 aggtggtgtt gacgaagtac tcaatagtgt tgccgccacc tttctgttcc ataatcgcct
     3241 gaccgatatg aataatttcg gcagcgcgct cgccaaagca gtgaataccc agaatctctt
     3301 ttgtttcccg atggaacaaa attttcagcg tgcccacgtt catgccgacg atttgtgcac
     3361 gtgccagatg tttaaactgg gcgcggccca cttcatatgg cactttcatc gcggttagct
     3421 gctgttcggt tttgcccaca gagctgattt ccgggatggt gtaaatgccg gtagggatat
     3481 cttcaatcag atgtgcgttg gcttcacctt tcaccagcgc ctgcgcggca atgcgcccct
     3541 ggtcataggc tgccgacgcc aggctcggat aaccaatcac gtcgcccacc gcgtaaacgt
     3601 gcggctgtgc ggtctgatac atgctgttga ccttcagctg tccgcgactg tcagtttcca
     3661 gcccaatgtt ctgtaacgcc agcgaatcag tattaccggt gcgaccgttg gcatagagca
     3721 ggcagtcagc tttcagtttt ttacccgact tcagatgcat gatcacaccg tcgtcacagc
     3781 cttcgatctt ctcgtactct tcgttgtgac gaatcactac gccactgttc cagaagtgat
     3841 aggagagaga atctgacatc tcttgatcga gaaacgccaa cagccgatcg cgggtgttga
     3901 tcagatccac ttttacatcc ataccgcgga agatcgacgc atattcacag ccgatcactc
     3961 cagcaccata gataagtaca tggcgcggtt cgtggtgcat gctaagaatt gagtcgctgt
     4021 cgtaaatgcg tggatgggtg aaatcaacat ctgttggatg atatggacga gagccgcagg
     4081 caataacaaa tttttcagcg gttagtgttt caacgctgcc gtccgggcaa tccagcgcca
     4141 gcgtgtgctc gtcaacaaag cgagcgtttc cctgcaatat ttcacagtga ttacgttcgt
     4201 aaaatccctg acgcatgcgc gtttgttgat taatcacgtt atcggcatgg ttaaggatat
     4261 cggcaaaaga agagcggagc agtcgcgaat ggtcgctgta aagtgggttt tgattaaatt
     4321 ctataatgcg gctgacggcg tgacggagag ctttcgacgg gatggtgccc cagtgggtgc
     4381 aaccgccgcc aacattttga taacgctcga taactgcgac gcgcgcacct tgcttaacca
     4441 ggcccattgc agcgccttcg ccgccggggc cggaacctat tactatggca tcgtaatcgt
     4501 aggaatgtgg catggtaggg cttacctgtt cttatacata aaagcaacag aatggtaaca
     4561 ttttatcgcg ggtaagccaa ttgatccccg tcatttatct ggctatatcg tgagcggcct
     4621 ttgctttgtc tgtttcttac ttttgacctg aacgttttat tggattttta tcgacgatac
     4681 tctccgttta agcggcaggt ttccgttgta cgtaaaagaa ccggccaaag aattgcagta
     4741 aatatgtttt attgcgttac cgttcattca caacactgga gcaatccagt atgttcattc
     4801 tctggtatag tgccagcagt acttttggca aggattcaga catcgtgatg ggcgtaagag
     4861 cgcaacaaaa agaaaaaacc cgccgttcgc tggtggaagc cgcatttagc caattaagtg
     4921 ctgaacgcag cttcgccagc ctgagtttgc gtgaagtggc gcgtgaagcg ggcattgctc
     4981 ccacctcttt ttatcggcat ttccgcgacg tagacgaact gggtctgacc atggttgatg
     5041 agagcggttt aatgctacgc caactcatgc gccaggcgcg tcagcgtatc gccaaaggcg
     5101 ggagtgtgat ccgcacctcg gtctccacat ttatggagtt catcggtaat aatcctaacg
     5161 ccttccggtt attattgcgg gaacgctccg gtacctccgc tgcgtttcgt gccgccgttg
     5221 cgcgtgaaat tcagcacttc attgcggaac ttgcggacta tctggaactc gaaaaccata
     5281 tgccgcgtgc gtttactgaa gcgcaagccg aagccatggt gacgattgtc ttcagtgcgg
     5341 gtgccgaggc gttggacgtc ggcgttgaac aacgtcggca attagaagag cgactggtac
     5401 tgcaactgcg aatgatttcg aaaggggctt attactggta tcgccgtgaa caagagaaaa
     5461 ccgcaattat tccgggaaat gtgaaggacg agtaatgaaa caagcaaatc aagatagagg
     5521 tacgctgctg ctggcgttag ttgctggctt atcgattaat ggtactttcg cagcgctgtt
     5581 tagctccatt gtgccatttt ctgtattccc gattatttcc ctggtgctga cggtttactg
     5641 cctgcatcaa cgttatctta atcgcactat gccggtaggc ttgccgggtc tggcagctgc
     5701 ctgttttatt ctcggcgtac tgctgtacag cacggtagtt cgtgcggagt atccggatat
     5761 cggctctaac ttcttcccgg cagtactgtc cgtcattatg gtgttctgga ttggcgcgaa
     5821 gatgcgtaac cgtaagcagg aagttgctga gtaatcggta tcatgccgga tgctgatgca
     5881 tccggcatgg gttttacttc gcggtcagta atacgccgca ctccatatgg tgcgtgtagg
     5941 ggaactgatc aaacagagcc agacgttcga ccttgtgcgt ctgacttaat gtttccagat
     6001 tcttgcataa cgtttccgga ttgcaggaga tatacagaat acgtggatac gcctgcacca
     6061 ttttctcggt ttcaccgtcc agaccgctgc gtggtgggtc gacaaaaatg gtttcgcact
     6121 gataactctt taaatcgatc ccttgcaggc ggttaaactc gcgtacgcca ttcatcgcct
     6181 gagtaaattc ttctgccgcc atacgaataa tttgtacgtt atcaatatgg ttagcggcaa
     6241 tgttgtattg cgcggcagcg accgacggct tagcgatttc ggtggctaat acccgatcaa
     6301 aattgcgcgc cagtgctaat gaaaagttac cgttgccgca gtacagctcc agtaaatcgc
     6361 cttttgagcc tttggttacg tccagcgccc attccagcat ctgaatattc atcgccgcgt
     6421 tcggctgggt aaagctgttt tctacctgac ggtagatcat ctctttccct gcgaccggca
     6481 gacgttcatc gatgtaatct tgatccagct cgattttggt tttcgttgcc cgaccaatca
     6541 gatgcacatt cagattctgc gctcgcagtg cgtcgcgcag ggcttccgct tcctgacgcc
     6601 actcatcatc cagcttctta tggtacagca gggaaaccac cgcctgatta ctcagcgtgg
     6661 tgaggtaatc aatctggaac aacttgtggc gcagaacggg attattacgc acacccgcaa
     6721 tcatcgccgt catcaactgg ttgataagtt cactggcggc ggggaagcta tccacgcgga
     6781 tgcggctttt ggtttgttga tcgaaaatga tgtgatacag gtcatcgcca tcgtgccaga
     6841 tgcggaactc cgcacgcatc cggtaatgac tgaccggcga gcgaaacact tccggaacca
     6901 ggtcagaaaa cggtgccatc atactttgca aacgtaccac tttttcggct aactgcgctt
     6961 catactgttc tgttggaagg tgttcggggg tcatgatgta tcctgaaaaa ttaaagtacg
     7021 cggcgattgt agggattgct catcagatgt ccagatcttg atgaattcct atttgtgagc
     7081 tacgtctgga cagtaacttg ttacaacctg tagcatccac ttgccggtcc tgtgagttaa
     7141 tagggaatcc agtgcgaatc tggagctgac gcgcagcggt aaggaaaggt gcgatgattg
     7201 cgttatgcgg acactgccat ccggtgggaa gtcatcatct cttagtatct tagatacccc
     7261 tccaagcccg aagacctgcc ggctaacgtc gcatctggtt ctcatcatcg cgtaatattg
     7321 atgaaacctg cggcatcctt cttctattgt ggatgcttta caatgattaa aaaagcttcg
     7381 ctgctgacgg cgtgttctgt cacagccttt tctgcttggg cacaggatac cagcccggat
     7441 actctcgtcg ttactgctaa ccgttttgaa cagccgcgca gcactgtgct tgcaccaacc
     7501 accgttgtga cccgtcagga tatcgaccgc tggcagtcga cctcggtcaa tgatgtgctg
     7561 cgccgtcttc cgggcgtcga tatcacccaa aacggcggtt caggtcagct ctcatctatt
     7621 tttattcgcg gtacaaatgc cagtcatgtg ctggtgttaa ttgatggcgt ccgcctgaat
     7681 ctggcgggag tgagtggttc tgccgacctt agccagttcc ctattgcgct tgtccagcgt
     7741 attgaatata tccgtggacc gcgctccgct gtttatggtt ccgatgcaat aggcggggtg
     7801 gtgaatatca tcacgacgcg cgatgaaccc ggaacggaaa tttcagcagg gtggggaagc
     7861 aatagttatc agaactatga tgtctccacg cagcaacaac tgggggataa gacacgggta
     7921 acgctgttgg gcgattatgc ccatactcat ggttatgatg ttgttgccta tggtaatacc
     7981 ggaacgcaag cgcagacaga taacgatggt tttttaagta aaacgcttta tggcgcgctg
     8041 gagcataact ttactgatgc ctggagcggc tttgtgcgcg gctatggcta tgataaccgt
     8101 accaattatg acgcgtatta ttctcccggc tcaccgttgc tcgatacccg taaactctat
     8161 agccaaagtt gggacgccgg gctgcgctat aacggtgaac tgattaaatc acaactcatt
     8221 accagctata gccatagcaa agattacaac tacgatcccc attatggtcg ttatgattcg
     8281 tccgcgacgc tggatgagat gaagcagtac accgtccagt gggcaaataa cgttaccgtt
     8341 ggccacggta atattggtgc gggcgttgac tggcagaaac agactacgac gccgggtact
     8401 ggttatgttg aggatgggta tgatcaacgt aacactggta tctatctgac cggattgcaa
     8461 caagtcggcg attttacatt tgaaggcgca gcccgcagcg acgataactc acagtttggt
     8521 cgtcatggaa cctggcaaac gagtgccggt tgggaattca tcgaaggcta tcgttttatc
     8581 gcttcttatg gcacgtcgta caaggcacct aatcttgggc aactgtatgg tttctacgga
     8641 aatccgaatc tgaatcctga gaaaagcaaa cagtgggaag gcgcgtttga aggcttaacc
     8701 gctggggtga actggcgtat ttccggatat cgtaacgatg tcagtgattt gatcgattat
     8761 gatgatcaca ccctgaaata ttacaacgaa gggaaagcgc ggattaaggg ggtcgaggcg
     8821 accgccaatt ttgataccgg accactgacg catactgtga gttatgatta tgtcgatgcg
     8881 cgtaatgcga ttaccgacgt gccgttgtta cgccgtgcta aacagcaggt gaaataccag
     8941 ctcgactggc aattgtatga cttcgactgg ggtattactt atcagtattt aggcactcgc
     9001 tatgataagg attactcatc ttatccttat caaaccgtta aaatgggcgg tgtgagcttg
     9061 tgggatcttg cggttgcgta tccggtcacc tctcatctga cagttcgtgg taaaatagcc
     9121 aacctgttcg acaaagatta tgagacagtc tatggctacc aaactgcagg acgggaatac
     9181 accttgtctg gcagctacac cttctgaacc acgtcccacc gtgctggtgt ttgactccgg
     9241 cgtcggtggg ttgtcggtct atgacgagat ccggcatctc ttaccggatc tccattacat
     9301 ttatgctttc gataacgtcg ctttcccgta tggcgaaaaa agcgaagcgt ttattgttga
     9361 gcgagtggtg gcaattgtca ccgcagtgca aaaacgttat ccccttgcgc tggctgtggt
     9421 cgcttgcaac actgccagta ccgtttcact tcctgcatta cgcgaaaagt tcgacttccc
     9481 ggttgttggt gtcgtgccgg cgattaaacc tgctgcacgt ctgacggcaa atggcattgt
     9541 cggattactg gcaacccgcg gaacagttaa acgttcttat actcatgagc tgatcgcgcg
     9601 tttcgctaat gaatgccaga tagaaatgct gggctcggca gagatggttg agttggctga
     9661 agcgaagctg catggcgaag atgtttctct ggatgcgcta aaacgtatct tgcgtccgtg
     9721 gttaagaatg aaagagccgc cagataccgt tgtactgggg tgtacccatt tccctctact
     9781 acaagaagaa ctgttacagg tgctgccaga gggaacccgg ctggtggatt ctggcgcagc
     9841 gattgctcgc cgaacggcct ggctgttaga acatgaagcc ccggatgcaa aatctgccga
     9901 tgcgaatatt gccttttgta tggcaatgac gccagaagct gaacaattat tgcccgtttt
     9961 acagcgttac ggcttcgaaa cgctcgaaaa actggcagtt ttaggctgat ttggttgaat
    10021 gttgcgcggt cagaaaatta ttttaaattt cctcttgtca ggccggaata actccctata
    10081 atgcgccacc actgacacgg aacaacggca aacacgccgc cg
//
LOCUS       DAMZFT010000169        10121 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.169, whole genome shotgun sequence.
ACCESSION   DAMZFT010000169 DAMZFT010000000
VERSION     DAMZFT010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10121)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10121)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10121
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.169"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(77..1024)
                     /locus_tag="PN977_004443"
     CDS             complement(77..1024)
                     /locus_tag="PN977_004443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001361319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HDI6044397.1"
                     /translation="MKNVLVTVPSFSARCVSASKLLRENNFNLIIKNNVEHLLKSEST
                     ALRESICAVIAGKDCYQADTLSLLSGVKIISRFGTGIDNIDLRAAQQSGIVVNNAVGI
                     NSNAVAEFIIGLIFASMRNIPGSYHAMQNGYWGESHGCELQGKRIGLLGYGNIGKTLA
                     KRLSGFDVELLAFDKQPDFQAAGKDGVQFVSIEDIFMQSHVIIVLLPFSPELENFISH
                     KYLSMMRNGALIINAARGKLLDEGALLQVIEERNVFAALDVFSSEPLAQFSPLLHAKN
                     IITTPHIAAATVESYQQTGIYVAQSIIDYFAGREIKNVL"
     gene            complement(1096..1692)
                     /locus_tag="PN977_004444"
     CDS             complement(1096..1692)
                     /locus_tag="PN977_004444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001683249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HDI6044398.1"
                     /translation="MNSTDLIHLIKHFMRNEHKAVEEVIDSPLSEVANLIKVLQSCQG
                     KVVFIGVGKSGIIARKLAATFASTGTPSFFVHGTEAVHGDLGMVAKDDVVILISNSGE
                     TAEILATLPSLKKMGNYLISFTRSHHSSLAISCDLSVEIPVKSEADNLGLAPSCSSTV
                     VLVVGDAVALALSELKKFTRADFGLYHPGGALGIKANS"
     gene            complement(1695..2870)
                     /locus_tag="PN977_004445"
     CDS             complement(1695..2870)
                     /locus_tag="PN977_004445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000382418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PatB family C-S lyase"
                     /protein_id="HDI6044399.1"
                     /translation="MDVFNTPISRKGTYCTQWDFCEDRFGVKDVLPFSISDMDLPIPD
                     AITRALKKRLEHPILGYSRWQHDDYLNAIVNWYHQQYKTEIKPEWITYSPSVMYSIAK
                     AIELLTSHGDNILVFTPVYNAFFDIIKHSERNILTASLIIKNEGGYAINWQDFDLKIQ
                     SAKMVLLCNPHNPTGTVWSEEELHKIAASCTRHNVWLCSDEIHSDFVFNRSFTSALKI
                     KKEKVVVFNSISKTFNVPALTGSYMISTDDNFNHKFRTISRYRDFVNSPSVLNIIATI
                     IAYNECEGWLKSLKAHIASNIQFTQQYLNENTPELIVRPADGCYFSWIDCSAIGYPFD
                     EFYSRLIHEGKVGIMAGNVYGTEGEGYLRLNLACGREKLYMGLTRLASVIKNINQGK"
     gene            complement(2870..4450)
                     /locus_tag="PN977_004446"
     CDS             complement(2870..4450)
                     /locus_tag="PN977_004446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000810568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDI6044400.1"
                     /translation="MKQKKAWSFFQSLGKAFMYPIALLSVCGMMLGLGSGLASDDMAK
                     LIPFLAIPIIKTILDFIVSLGLFAFVNLPVLFAIAIPLGLLKDKEDKAYGAFSGLIGF
                     MAMHLGTNFYLKQHDLLVAADQMSTHGQTIILGIQSYNTSVLGGIVAGLLVASMYKKI
                     VNLRIPESLGFYSGPRLVPIITLIVMSGFGLIIPFIWPPFFNLFMLIGHWISTSGPVG
                     YFFYAVAERVTIPFGLNHMVTSVFRFTPIGGSAVIGGEEYYGTLNMFMAYVKENAVIP
                     LDLAGKMEQGKLMIQYGLAGAALAMYRTAHAQNRKAIKALLISGVLTVIIGGVSEPIE
                     FLFLFVSPLLFVFHAFMNGFANMVLPYMGVKMGFTGDLIQFISFGVLRGTRTGWPIAV
                     CVEVAYFFIYYFVFRWTILKFNLMTVGREESSPVTLNAHEDTAIADIPTPDKSELQAA
                     EQMVKALGGKENIKSLDNCVTRLRLTIADMGLLDEAAIKRAGGIAVVKLDQNTLQVII
                     GTKVIALRRDMDNYMGIC"
     gene            complement(4482..5306)
                     /locus_tag="PN977_004447"
     CDS             complement(4482..5306)
                     /locus_tag="PN977_004447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001606056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HDI6044401.1"
                     /translation="MIIEKVMNNNCVQASMNGQEVIISGPGVGYNKKYGMSVPEHPAN
                     RIFYVRNEQKNKLYKLIEHVDIEYVFVAEKIVQYAENNLEKNLNPSLLLILADHISNA
                     ISRVASGIQINNVFLDEIKALYKAEYAISRDALTIINEQFSVQLPDDEIGFIALHILN
                     NYENSVDYESVRIIELSQIITGLIEVVYNRKVDRSSFNYSRFMIHLKYFSSRVLCNEK
                     IKQKDIGDIYEQFLEKDILLQRAIHEIERYLYATFKYELILEEKLYLSIRTKVLMD"
     gene            complement(5563..6816)
                     /gene="amiC"
                     /locus_tag="PN977_004448"
     CDS             complement(5563..6816)
                     /gene="amiC"
                     /locus_tag="PN977_004448"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiC"
                     /protein_id="HDI6044402.1"
                     /translation="MSGSNTAISRRRLLQGAGAMWLLSVSQVSLAAVSQVVAVRVWPA
                     SSYTRVTVESNRQLKYKQFALSNPERVVVDIEDVNLNSVLKGMAAQIRADDPFIKSAR
                     VGQFDPQTVRMVFELKQNVKPQLFALAPVAGFKERLVMDLYPANAQDMQDPLLALLED
                     YNKGDLEKQVPPAQSGPQPGKAGRDRPIVIMLDPGHGGEDSGAVGKYKTREKDVVLQI
                     ARRLRSLIEKEGNMKVYMTRNEDIFIPLQVRVAKAQKQRADLFVSIHADAFTSRQPSG
                     SSVFALSTKGATSTAAKYLAQTQNASDLIGGVSKSGDRYVDHTMFDMVQSLTIADSLK
                     FGKAVLNKLGKINKLHKNQVEQAGFAVLKAPDIPSILVETAFISNVEEERKLKTATFQ
                     QEVAESILAGIKAYFADGATLARRG"
     gene            7048..8379
                     /gene="argA"
                     /locus_tag="PN977_004449"
     CDS             7048..8379
                     /gene="argA"
                     /locus_tag="PN977_004449"
                     /EC_number="2.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HDI6044403.1"
                     /translation="MVKERKTELVEGFRHSVPYINTHRGKTFVIMLGGEAIEHENFSS
                     IVNDIGLLHSLGIRLVVVYGARPQIDANLAAHHHEPLYHKNIRVTDAKTLELVKQAAG
                     TLQLDITARLSMSLNNTPLQGAHINVVSGNFIIAQPLGVDDGVDYCHSGRIRRIDEDA
                     IHRQLDSGAIVLLGPVAVSVTGESFNLTSEEIATQLAIKLKAEKMIGFCSSQGVTNDD
                     GDIVSELFPNEAQARVEAQEEKGDYNSGTVRFLRGAVKACRSGVRRCHLISYQEDGAL
                     LQELFSRDGIGTQIVMESAEQIRRATINDIGGILELIRPLEQQGILVRRSREQLEMEI
                     DKFTIIQRDNTTIACAALYPFPEEKIGEMACVAVHPDYRSSSRGEVLLERIAAQAKQS
                     GLSKLFVLTTRSIHWFQERGFTPVDIDLLPESKKQLYNYQRKSKVLMADLG"
     gene            complement(8441..>10121)
                     /gene="recD"
                     /locus_tag="PN977_004450"
     CDS             complement(8441..>10121)
                     /gene="recD"
                     /locus_tag="PN977_004450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000775924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit alpha"
                     /protein_id="HDI6044404.1"
                     /translation="HVCLPLSRLENNEASHPLLATCVSEIGELQNWEECLLASQAVSR
                     GDEPTPMILCGDRLYLNRMWCNERTVARFFNEVNHAIEVDEALLAQTLDKLFPVSDDI
                     NWQKVAAAVALTRRISVISGGPGTGKTTTVAKLLAALIQMADGERCRIRLAAPTGKAA
                     ARLTESLGKALRQLPLTDEQKKRIPEDASTLHRLLGAQPGSQRLRHHAGNPLHLDVLV
                     VDEASMIDLPMMSRLIDALPDHARVIFLGDRDQLASVEAGAVLGDICAYANAGFTAER
                     AGQLSRLTGTHIPSGTGTEAASLRDSLCLLQKSYRFGSDSGIGQLAAAINRGDKMAVK
                     TVFQQDFTDIEKRLLQSGEDYIAMLEEALSGYGRYLDLLQARAEPDLIIQAFNEYQLL
                     CALREGPFGVAGLNERIEQFMQQKRKIHRNPHSRWYEGRPVMIARNDSALGLFNGDIG
                     IALDRGQGTRVWFAMPDGNIKSVQPSRLPEHETTWAMTVHKSQGSEFDHAALILPSQR
                     TPVVTRELVYTAVTRARRRLSLYADERILSAAIATRTERRSGLAALFSSRG"
BASE COUNT         2796 a         2487 c         2175 g         2663 t
ORIGIN      
        1 acttatgatg ctgataaacc gcaatgtgtt tcacaaattg cggttttttt gtacctgaaa
       61 tattccttag ctgaactcac agcacgtttt ttatctccct tcctgcgaag taatcaatta
      121 tcgactgggc aacgtatatc ccggtttgct ggtatgactc aacggtggcg gccgcaatat
      181 gcggcgttgt aatgatattc ttggcatgta gcaatgggct aaattgcgct agcggttcag
      241 agctaaacac atccagtgct gcaaaaacat ttcgttcttc aattacctga agtaaagcac
      301 cctcatccag caactttccc cttgcggcgt tgatgatcag agcaccattg cgcatcatcg
      361 acaaatattt atgactaatg aagttctcca gctccggaga aaatggcaaa agaacgatga
      421 tgacatgaga ctgcataaag atatcttcaa ttgatacgaa ctgaactcca tctttgcctg
      481 cggcctggaa atcgggttgt ttgtcaaaag ctaaaagctc aacatcaaaa ccagataacc
      541 ttttcgccag tgttttgcca atattgccgt agccaagcag gccaatacgt ttaccttgta
      601 actcgcagcc atgcgactcg ccccaataac cattttgcat cgcatgatag ctaccgggga
      661 tatttctcat gctcgcgaag ataagtccaa tgataaattc cgcaaccgca tttgaattaa
      721 tcccaaccgc attattgaca acaataccgg attgctgtgc cgcacgaaga tcaatattgt
      781 ctatccccgt cccgaatctc gatattattt tcacaccaga cagcaatgat agcgtgtccg
      841 cctgataaca atcttttccg gcgatgaccg cgcaaataga ttctcgtaat gcagtggatt
      901 ctgatttaag cagatgctcc acattattct taataataag attaaaatta ttctctctta
      961 gcaacttgct ggcggaaaca cagcgtgctg aaaagctggg gacggtgacc agaacatttt
     1021 tcatagctga cactcacttc aaagaacgcc accccaaaag atggcgtgac cggttactgt
     1081 atttatattt tgaaattatg agtttgcttt aataccgagc gcacctcctg gatgatataa
     1141 gccgaaatca gcacgagtaa actttttcaa ttcggagagc gcaagcgcca ccgcatcacc
     1201 aacaaccaga acgactgttg atgagcatga cggcgctaaa cctaaattat ccgcctcact
     1261 ttttacggga atttcaacgg ataaatcaca acttatcgca agcgatgagt gatgactgcg
     1321 tgtaaaagag atcaaataat tacccatttt tttcagacta ggcagtgtcg ccaggatttc
     1381 cgccgtctca ccactgtttg aaataagaat aacaacatcg tctttcgcca ccattccaag
     1441 gtcgccgtgt accgcttccg taccgtgaac aaaaaacgag ggagttccag tgctggcaaa
     1501 tgttgcggcg agttttcggg caataatacc ggacttaccg acaccaataa aaacaacttt
     1561 cccctgacaa gactgtaata ctttaattaa atttgcgacc tccgaaaggg gggaatcaat
     1621 aacttcttca actgctttat gttcgttacg cataaaatgt ttaatgagat ggataagatc
     1681 cgtgctgttc atttttattt tccttgatta atatttttaa ttacactcgc aagacgcgta
     1741 agccccatat ataatttttc tcgaccgcag gccaggttca gacgaaggta gccctccccc
     1801 tccgttccat agacattccc cgccataatg cccactttgc cttcatgaat taatcgactg
     1861 tagaattcat caaagggata accaatagca gagcaatcta tccacgaaaa atagcaacca
     1921 tcagctggcc ttacgatcaa ttctggagtg ttttcgttca gatactgctg ggtgaattga
     1981 atgttagagg caatatgtgc ctttaatgac tttaaccatc cctcacattc gttataggca
     2041 ataatagttg caattatatt cagtactgat ggggagttta caaaatcgcg atacctcgat
     2101 atcgttctga acttatgatt aaaattatcg tcagttgaaa tcatatacga tcctgttagt
     2161 gcaggaacat taaatgtttt cgatatcgag ttaaatacta ctactttttc tttttttatt
     2221 ttaagcgcgg atgtaaagct acgattaaaa acaaaatcac tatggatttc atctgaacat
     2281 aaccagacat tatgccttgt acagcttgca gcgattttat gcagttcttc ttccgaccag
     2341 actgttcctg tcgggttatg aggattacat aataacacca tctttgcgct ttgaatctta
     2401 agatcaaaat cttgccaatt gatagcgtag cccccctcat tttttataat caaagaggct
     2461 gtcagaatat ttctctcgct atgtttaatt atatcaaaaa atgcattata gacaggtgta
     2521 aaaacgagaa tattatcacc atgcgatgtc aataactcta ttgctttagc aatggagtac
     2581 atcacgcttg ggctataggt gatccattct ggtttgattt cagttttata ttgttgatga
     2641 taccaattga ctatcgcatt cagatagtca tcatgctgcc aacggctgta ccctaaaatc
     2701 gggtgttcca gacgtttttt taatgctctg gtgatagcgt ctggtatcgg aagatccata
     2761 tctgaaatag agaatggtaa tacatctttt acaccaaatc tgtcctcgca aaagtcccat
     2821 tgggtacaat atgttccttt gcgggaaata ggagtattaa aaacatccat cagcatatcc
     2881 ccatgtagtt atccatatcc cgacgcaagg cgatgacttt agtaccgata atgacttgta
     2941 gggtattttg atcaagttta acaaccgcaa ttccgccggc tctttttatt gcagcctcgt
     3001 caagcaatcc catatctgcg attgttaaac gtaaacgagt tacgcaatta tccagtgact
     3061 taatattctc tttaccacca agtgccttaa ccatctgctc cgccgcttgc agctctgatt
     3121 tatcaggagt tgggatatcc gctatagccg tatcctcatg agcgttcagc gtgacaggac
     3181 tggactcttc acggcctacc gtcatcaggt taaatttgag aatagtccaa cggaacacaa
     3241 agtaataaat aaagaaatag gccacttcga cacacaccgc tattggccaa cctgttcttg
     3301 tgccacgcaa tacgccaaag ctaataaatt gaatcagatc gccagtaaat cccatcttca
     3361 cgcccatata tggcaagacc atgttggcga atccattcat aaatgcatgg aagacaaaca
     3421 gcagtggact gacaaataag aacagaaact caatcggttc gctgacgccg ccaataatga
     3481 ctgtaagcac cccggaaata agcaatgctt tgatagcttt tctgttttga gcatgagcag
     3541 tgcgatacat cgccagcgcg gcaccagcca gaccatactg aatcatcagt ttgccctgtt
     3601 ccattttccc cgccaaatcc agcggaatga ccgcattctc tttgacgtac gccataaaca
     3661 tgttcagggt gccataatac tcttcgccgc cgatcactgc cgatccgccg atgggggtaa
     3721 agcggaaaac tgacgtcacc atgtgattta aaccaaaagg tatcgtcacg cgttcggcga
     3781 ctgcatagaa aaaatagcca acaggaccgg aagttgaaat ccagtggcca atgagcatga
     3841 aaagattgaa aaacggcggc cagataaaag ggatgatcag accaaatcca ctcatcacaa
     3901 tcagtgtaat gataggcacc agacgtgggc cgctataaaa acctaatgat tcaggaatgc
     3961 gtaaattaac gatcttttta tacatgctgg cgactaataa cccagcaaca attcccccta
     4021 acacgctggt attgtaggac tggatcccca gaatgatggt ttgcccatgt gtcgacattt
     4081 ggtcagcagc gaccaataag tcgtgctgtt taagataaaa gttcgttccc agatgcatcg
     4141 ccataaaacc aattaagcca gaaaaagcac cataggcttt atcctcttta tcttttaata
     4201 atcctaaggg aatcgctatc gcaaacaata caggtaaatt aacaaaggca aacaaaccaa
     4261 gactaacaat gaaatcaagt atggttttaa ttattggaat agccagaaat ggaattaact
     4321 ttgccatatc atcactggct aaaccacttc ccagccctag catcatgcca catacactta
     4381 gcagagcaat gggatacatg aatgcctttc ccaggctctg aaaaaaactc caggctttct
     4441 tttgtttcat gtgggttatc tcatataaat gttatatata attagtccat taatactttg
     4501 gtacgaattg agagatatag tttttcttct aaaattaatt catatttaaa agtggcatac
     4561 agataccgtt caatttcatg aattgcgcgc tgtaacagga tgtctttctc aagaaactgc
     4621 tcatagatgt caccaatatc tttttgtttt atcttttcat tacataatac gcgacttgaa
     4681 aaatatttaa ggtgtatcat gaacctggaa taattaaatg aacttctgtc cacctttctg
     4741 ttataaacaa cttcaataag ccccgtgatt atttgcgaca actcaataat ccgtactgat
     4801 tcataatcaa ctgaattttc ataattattt aaaatatgca atgctataaa accaatctca
     4861 tcatcaggaa gttgaacgct gaattgctca ttaatgatag ttaatgcatc gcgacttatc
     4921 gcatactctg ctttgtacaa cgctttgatt tcatcaagga aaacattatt aatttgtatg
     4981 cctgaagcga ctcgggatat tgcattcgaa atgtgatcag caagaatcaa tagtagcgat
     5041 ggattgagat ttttttcgag attgttctcc gcatattgca ctattttttc ggcaacaaaa
     5101 acatactcaa tatccacatg ttcaatcaat ttgtagagtt tatttttttg ttcatttctg
     5161 acataaaaaa tccggttagc cggatgctca gggaccgaca ttccatattt tttgttgtaa
     5221 ccgacgccag gcccagaaat gataacctcc tgtccattca tcgatgcctg tacacaatta
     5281 ttgttcatga ctttttcgat gatcatttca tggctccaaa aaaaaggcaa ggcacgtcag
     5341 aagtaatgat tacttcaaac attgccttgc ctgatttaac agttacaaga tgattaacta
     5401 atgcgtatac attacacgga attgtgctta attaaagaga tggtcggctt atttttcaag
     5461 ccgacattca aatatccgac atcagtcaca aaaccagcgt ctaatattca ggtaaatgtt
     5521 atctattcaa cgaaatataa attcattgaa taaataaaaa cattatcccc ttctcgccag
     5581 cgtcgcccca tcggcaaaat acgctttaat tcccgcaaga atagactccg caacttcctg
     5641 ctggaaagtc gccgttttca gtttacgctc ttcctcaacg ttactgataa acgccgtttc
     5701 gaccagaatg gagggaatat ctggtgcctt tagtacggca aacccggcct gttcaacttg
     5761 atttttatgc agcttgttga ttttaccgag cttattcagc accgctttac caaacttcag
     5821 gctgtcagca atggtcagcg actgcaccat atcgaacata gtgtggtcga catagcggtc
     5881 accgcttttg cttacgccac caatcaagtc cgaggcgttc tgggtttgtg ccagatattt
     5941 tgccgcagta ctggttgcgc ctttggttga gagcgcaaac accgaggaac cgctcggctg
     6001 gcgactggta aaggcgtcgg catggataga gacaaacaag tcagcacgct gtttctgggc
     6061 ttttgctacg cgcacttgca acggaatgaa gatgtcttca ttgcgcgtca tgtacacctt
     6121 catattgccc tctttctcga tcagagagcg cagacggcga gctatttgca atactacgtc
     6181 tttttcgcgt gttttgtatt tccccaccgc accggagtct tcgccaccgt gaccagggtc
     6241 aagcataatg acaatcggac gatcccgccc agctttaccc ggttgtggac cactttgtgc
     6301 tggcggcacc tgcttttcga ggtcgccttt gttgtaatcc tccagcagcg ccagcagcgg
     6361 gtcctgcata tcctgtgcat tggcaggata gagatccatc accagacgct ctttaaaccc
     6421 ggcgaccggc gcaagggcaa acagctgcgg ttttacgttt tgctttaatt caaaaaccat
     6481 acgtacggtt tgcgggtcaa attgcccgac gcgcgccgac ttgatgaacg ggtcgtcagc
     6541 gcggatttgc gcagccatcc ccttgagcac cgagttcagg tttacatctt cgatatccac
     6601 caccacgcgt tcaggattac tcaacgcgaa ctgcttatat ttcagctgac gattagattc
     6661 taccgtcacg cgggtgtagc tggacgcagg ccagacgcgc accgccacga cctggctgac
     6721 cgcagccagg ctgacctgac ttacgctcaa tagccacatg gcacccgcgc cttgcagtaa
     6781 acgacgacgg ctgattgcag tgttggatcc tgacatgcct ctcccgagca aaagaaatct
     6841 aagctgtgta acaagtaaac gaccaatttg accggaaact ttagcgaaag acgcataatc
     6901 tgtcatctat aaaacggtaa acattctttt tatattcacg gtattactga taaaaaagtc
     6961 gctcccgcat aaaatttaca cttgcaccct gcgaaaaaac agaataaaaa tacaataatt
     7021 tcgaataatc atgcaaagag gtgtgccgtg gtaaaggaac gtaaaaccga gttggtcgag
     7081 ggattccgcc attcggttcc ctatatcaat acccaccggg gaaaaacgtt tgtcataatg
     7141 ctcggcggtg aagccattga gcatgagaat ttctccagta tcgttaatga tatcgggttg
     7201 ctgcacagcc tcggcatccg tctggtcgtg gtctatggag cgcgcccgca gatcgatgca
     7261 aatctggcag cgcatcacca cgaaccgcta tatcacaaaa atatacgcgt gaccgacgcc
     7321 aaaacactgg aactggtgaa gcaggctgcg ggaacattgc aactggatat tactgctcgc
     7381 ctgtcgatga gtctcaataa cacgccgctg cagggcgcgc atatcaacgt cgtcagtggc
     7441 aattttatta ttgcccagcc gctgggcgtg gatgacggtg tggattactg ccatagcggg
     7501 cgtatccggc ggattgacga agatgcgatt catcgtcaac tggacagcgg tgcgatagtg
     7561 ctgctggggc cggtcgcggt ttcagtcact ggcgagagct ttaatctgac ctcggaagag
     7621 attgccactc aactggccat caaactgaaa gctgagaaga tgattggttt ttgctcttca
     7681 cagggcgtca ctaatgacga cggtgatatt gtctcagaac ttttccctaa cgaagcgcaa
     7741 gcgcgggtag aagcccagga agagaaaggc gattacaact ccggtacggt gcgctttttg
     7801 cgtggcgcag tgaaagcctg ccgcagcggc gtgcgtcgct gtcatttaat cagttatcag
     7861 gaagatggcg cgctgttgca agagttgttc tcacgtgacg gtatcggtac gcagattgtg
     7921 atggaaagcg ccgagcaaat tcgtcgcgca acaatcaacg atattggcgg cattctggag
     7981 ttgattcgcc cactggagca gcaaggtatt ctggtacgcc gttctcgcga gcagctggag
     8041 atggaaatcg acaaattcac cattattcag cgcgataaca cgactattgc ctgcgccgcg
     8101 ctctatccgt tcccggaaga gaagattggg gaaatggcct gtgtggcagt tcacccggat
     8161 taccgcagct catcacgggg cgaggttctg ctggaacgca ttgccgctca ggcgaagcag
     8221 agcggcttaa gcaaattgtt tgtgctgacc acgcgcagta ttcactggtt ccaggaacgt
     8281 ggatttaccc cagtggatat tgatttactg cccgagagca aaaagcagtt gtacaactac
     8341 cagcgtaaat ccaaagtttt gatggcggat ttagggtaag cgatgaaaat cgccggatgc
     8401 gacatgcgta acactcgtgc gtcgcatccg gcaattacgt ttatccccgt gaactaaaca
     8461 acgccgccag accgctgcgc cgctcggtgc gcgtggcaat tgccgcactt aaaatgcgct
     8521 catcggcata cagcgacaga cggcgacgcg cgcgggtcac cgcagtgtaa accagctctc
     8581 gtgttactac tggcgtgcgt tggctcggta aaatcaacgc cgcatggtcg aactccgatc
     8641 cctgcgattt atgtaccgtc atcgcccacg tcgtttcgtg ctctggcaag cgactcggtt
     8701 gcacagactt aatattgccg tccggcatcg caaaccagac gcgcgtcccc tgcccgcgat
     8761 ccagcgcaat accgatatcg ccattaaaca acccaagcgc gctgtcatta cgggcaatca
     8821 tcaccggtcg gccttcgtac caacgagagt gcggattacg atgaattttg cgcttctgtt
     8881 gcataaactg ctcaattcgc tcattcagtc cagccacgcc aaacggccct tcccgcaggg
     8941 cgcacaaaag ctggtactca ttgaacgcct gaatgattaa atccggctcg gcacgcgctt
     9001 gcagcagatc cagataacgt ccgtaacccg aaagagcttc ctcaagcatc gcaatataat
     9061 cttcgccgct ctgtaaaagc cgtttttcga tatcagtaaa atcctgctga aaaacggttt
     9121 tcactgccat tttatcacca cggttaatcg ccgcagctaa ctgaccaatg ccagaatcgc
     9181 tgccgaaacg atagcttttt tgcagcaggc agagactgtc gcgcaaagat gccgcttctg
     9241 tgccagttcc tgacggaatg tgagttcccg tcaggcggct tagctgcccg gcacgctcgg
     9301 cggtaaagcc ggcattggca taagcacaaa tatcgcccag cacagcccca gcctcaaccg
     9361 aggccagttg atcacgatcg ccgagaaaga tcactcgcgc atgatcgggc aaggcgtcta
     9421 tcagtctcga catcatcggc agatcgatca ttgacgcttc atctactacc agcacatcaa
     9481 gatgcagcgg gttaccggca tgatgacgta aacgctggct acccggctgt gcgcccagca
     9541 atcggtgcaa agtactggca tcttccggaa tgcgtttctt ttgttcatcg gtcagcggta
     9601 actggcgcaa agccttgccg agagattcgg ttaagcgcgc ggcagcttta cccgttggtg
     9661 cagccagacg gatacggcag cgttctccgt cggccatttg aattaaggct gccagcaact
     9721 tcgctacggt ggtcgtttta ccggtgccag gaccgccgga aatcaccgaa atccgccgcg
     9781 tcagcgccac tgccgccgcc actttttgcc agttaatgtc atcgcttact ggaaaaagtt
     9841 tgtccagggt ttgcgccagt agagcttcat caacctcaat ggcatgattc acttcgttga
     9901 aaaagcgtgc cactgtgcgc tcgttacacc acatgcgatt caaataaaga cgatcgccac
     9961 agaggatcat cggcgttggt tcatctcccc ggctgaccgc ttgtgaagcc agcaagcatt
    10021 cttcccaatt ttgtagctca ccgatttcac tgacacaggt cgccaacagc ggatgcgatg
    10081 cctcgttatt ttccagtcgt gaaagcggca aacaaacgtg t
//
LOCUS       DAMZFT010000170        10080 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.170, whole genome shotgun sequence.
ACCESSION   DAMZFT010000170 DAMZFT010000000
VERSION     DAMZFT010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10080)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10080)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10080
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.170"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(<1..156)
                     /locus_tag="PN977_004451"
     CDS             complement(<1..156)
                     /locus_tag="PN977_004451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6044405.1"
                     /translation="MSTQYETQGYTINNAGRRLVVDPITRIEGHMRCEVNINDQNVIT
                     NAVSCGTM"
     gene            complement(153..1271)
                     /gene="hyaA"
                     /locus_tag="PN977_004452"
     CDS             complement(153..1271)
                     /gene="hyaA"
                     /locus_tag="PN977_004452"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 small subunit"
                     /protein_id="HDI6044406.1"
                     /translation="MNNEETFYQAMRRQGVTRRSFLKYCSLAATSLGLGAGMAPKIAW
                     ALENKPRIPVVWIHGLECTCCTESFIRSAHPLAKDVILSLISLDYDDTLMAAAGTQAE
                     EVFEDIITQYNGKYILAVEGNPPLGEQGMFCISSGRPFIEKLKRAAAGASAIIAWGTC
                     ASWGCVQAARPNPTQATPIDKVITDKPIIKVPGCPPIPDVMSAIITYMVTFDRLPDVD
                     RMGRPLMFYGQRIHDKCYRRAHFDAGEFVQSWDDDAARKGYCLYKMGCKGPTTYNACS
                     STRWNDGVSFPIQSGHGCLGCAENGFWDRGSFYSRVVDIPQMGTHSTADTVGLTALGV
                     VAAAVGVHAVASAVDQRRRHNQQPTETEHQPGNEDKQA"
     gene            1698..1785
                     /locus_tag="PN977_004453"
     tRNA            1698..1785
                     /locus_tag="PN977_004453"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1732..1734,aa:Ser,seq:tga)
     gene            1992..2651
                     /gene="yccA"
                     /locus_tag="PN977_004454"
     CDS             1992..2651
                     /gene="yccA"
                     /locus_tag="PN977_004454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000375124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease modulator YccA"
                     /protein_id="HDI6044407.1"
                     /translation="MDRIVSSSHDRTSLLSTHKVLRNTYFLLSLTLAFSAITATASTV
                     LMLPSPGLILTLVGMYGLMFLTYKTANKPTGIISAFAFTGFLGYILGPILNTYLSAGM
                     GDVIAMALGGTALVFFCCSAYVLTTRKDMSFLGGMLMAGIVVVLIGMVANIFLQLPAL
                     HLAISAVFILISSGAILFETSNIIHGGETNYIRATVSLYVSLYNIFVSLLSILGFASR
                     D"
     gene            2743..3072
                     /gene="tusE"
                     /locus_tag="PN977_004455"
     CDS             2743..3072
                     /gene="tusE"
                     /locus_tag="PN977_004455"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusE"
                     /protein_id="HDI6044408.1"
                     /translation="MLIFEGKEIETDTEGYLKESSQWSEPLAVVIAENEGIALSPEHW
                     EVVRFVRDFYLEFNTSPAIRMLVKAMANKFGEEKGNSRYLYRLFPKGPAKQATKIAGL
                     PKPVKCI"
     gene            complement(3069..3347)
                     /gene="yccX"
                     /locus_tag="PN977_004456"
     CDS             complement(3069..3347)
                     /gene="yccX"
                     /locus_tag="PN977_004456"
                     /EC_number="3.6.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000048233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HDI6044409.1"
                     /translation="MSKVCIIAWVYGRVQGVGFRYTTQYEAKKLGLTGYAKNLDDGSV
                     EVVACGEEGQVEKLMQWLKSGGPRSARVERVLSEPHHPSGELTDFRIH"
     gene            3442..4632
                     /gene="rlmI"
                     /locus_tag="PN977_004457"
     CDS             3442..4632
                     /gene="rlmI"
                     /locus_tag="PN977_004457"
                     /EC_number="2.1.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415487.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytosine(1962)-C(5))-methyltransferase
                     RlmI"
                     /protein_id="HDI6044410.1"
                     /translation="MSVRLVLAKGREKSLLRRHPWIFSGAVARMEGKASLGETIDIVD
                     HQGKWLARGAYSPASQIRARVWTFDPSESIDIAFFTRRLQQAQKWRDWLAQKDGLNSY
                     RLIAGESDGLPGITIDRFGNFLVLQLLSAGAEYQRAALISALQTLYPECAIYDRSDVA
                     VRKKEGMELTQGPVTGELPPALLPIEEHGMKLLVDIQHGHKTGYYLDQRDSRLATRRY
                     VENKRVLNCFSYTGGFAVSALMGGCSQVVSVDTSQEALDIARQNVELNKLDLSKAEFV
                     RDDVFKLLRTYRDRGEKFDVIVMDPPKFVENKSQLMGACRGYKDINMLAIQLLNEGGV
                     LLTFSCSGLMTSDLFQKIIADAAIDAGRDVQFIEQFRQAADHPVIATYPEGLYLKGFA
                     CRVM"
     gene            4690..5007
                     /gene="hspQ"
                     /locus_tag="PN977_004458"
     CDS             4690..5007
                     /gene="hspQ"
                     /locus_tag="PN977_004458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HspQ"
                     /protein_id="HDI6044411.1"
                     /translation="MIASKFGIGQQVRHSLLGYLGVVVDIDPVYSLSEPSPDELAVND
                     ELRAAPWYHVVMEDDNGLPVHTYLAEAQLSSELQDEHPEQPSMDELAQTIRKQLQAPR
                     LRN"
     gene            complement(5052..5465)
                     /locus_tag="PN977_004459"
     CDS             complement(5052..5465)
                     /locus_tag="PN977_004459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HDI6044412.1"
                     /translation="MKETDIAGILTSTHTIALVGASDKPDRPSYRVMKYLLDQGYHVI
                     PVSPKVAGKTLLGQQGYGTLAEVPEKVDMVDVFRNSEAAWGVAQEAIAIGAKTLWMQL
                     GVINEQAAVLARDAGLNVVMDRCPAIEIPRLGLAK"
     gene            5638..6300
                     /locus_tag="PN977_004460"
     CDS             5638..6300
                     /locus_tag="PN977_004460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2057 family protein"
                     /protein_id="HDI6044413.1"
                     /translation="MKTGIVTTLIALCLPVSVFATTLRLSTDVDLLVLDGKKVSSSLL
                     RGADSIELDNGPHQLVFRVEKTIHLSNSEERLYISPPLVVSFNTQLINQVNFRLPRLE
                     NEREANHFDAAPRLELLDGDATPIPVKLDILAITSTAKTIDYEVEVERYNKSAKRASL
                     PQFATMMADDSTLLSGVSELDAIPPQSQVLTEQRLKYWFKLADPQTRNTFLQWAEKQP
                     SS"
     gene            6397..6855
                     /gene="mgsA"
                     /locus_tag="PN977_004461"
     CDS             6397..6855
                     /gene="mgsA"
                     /locus_tag="PN977_004461"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HDI6044414.1"
                     /translation="MELTTRTLPARKHIALVAHDHCKQMLMSWVERHQPLLEQHVLYA
                     TGTTGNLISRATGMNVNAMLSGPMGGDQQVGALISEGKIDVLIFFWDPLNAVPHDPDV
                     KALLRLATVWNIPVATNVATADFIIQSPHFNDAVDILIPDYQRYLADRLK"
     gene            complement(6887..8941)
                     /gene="helD"
                     /locus_tag="PN977_004462"
     CDS             complement(6887..8941)
                     /gene="helD"
                     /locus_tag="PN977_004462"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase IV"
                     /protein_id="HDI6044415.1"
                     /translation="MELKATTLGKRLAQHPYDRAVILNAGIKVSGDRHEYLIPFNQLL
                     AIHCKRGLVWGELEFVLPDEKVVRLHGTEWGETQRFYHHLDTHWRRWSGEMSEIASGV
                     LRQQLDLIATRTGENKWLTREQTSGVQQQIRQALSALPLPVNRLEEFDNCREAWRKCQ
                     AWLKDIEGARLQHNQAYTEAMLTEYADFFRQVESSPLNPAQARAVVNGEHSLLVLAGA
                     GSGKTSVLVARAGWLLARGEASPEQILLLAFGRKAAEEMDERIRERLHTEDITARTFH
                     ALALHIIQQGSKKVPIVSKLENDTAARHELFIAEWRKQCSEKKAQAKGWRQWLTEEMQ
                     WSVPEGNFWDDEKLQRRLASRLDRWVSLMRMHGGAQAEMIASAPEEIRDLFSKRIKLM
                     APLLKAWKGALKAENAVDFSGLIHQAIVILEKGRFISPWKHILVDEFQDISPQRAALL
                     AALRKQNSQTTLFAVGDDWQAIYRFSGAQMSLTTAFHENFGEGDRCDLDTTYRFNSRI
                     GEVANRFIQQNPGQLKKPLNSLTNGDKKAVTLLDESQLDALLDKLSGYAKPEERILIL
                     ARYHHMRPASLEKAATRWPKLQIDFMTIHASKGQQADYVIIVGLQEGSDGFPAAARES
                     IMEEALLPPVEDFPDAEERRLMYVALTRARHRVWALFNKENPSPFVEILKNLDVPVAR
                     KP"
     gene            9064..9510
                     /locus_tag="PN977_004463"
     CDS             9064..9510
                     /locus_tag="PN977_004463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HDI6044416.1"
                     /translation="MRTVLNILNFVLGGFATTLGWLLATLVSIVLIFTLPLTRSCWEI
                     TKLSLVPYGNEAIHVDELNPAGKNVLLNTGGTVLNIFWLIFFGWWLCLMHIATGIAQC
                     ISIIGIPVGIANFKIAAIALWPVGRRVVSVETAQAAREANARRRFE"
     gene            9520..>10080
                     /locus_tag="PN977_004464"
     CDS             9520..>10080
                     /locus_tag="PN977_004464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01666 family membrane protein"
                     /protein_id="HDI6044417.1"
                     /translation="MAFMLSPLLKRYTWNSAWLYYARIFIALCGTTAFPWWLGDVKLT
                     IPLTLGMVAAALTDLDDRLAGRLRNLIITLFCFFIASASVELLFPWPWLFAIGLTLST
                     SGFILLGGLGQRYATIAFGALLIAIYTMLGTSLYEHWYQQPMYLLAGAVWYNVLTLIG
                     HLLFPVRPLQDNLARCYEQLARYLELK"
BASE COUNT         2310 a         2628 c         2543 g         2599 t
ORIGIN      
        1 catggtgccg caggagacgg cattggtgat cacattctga tcgttaatat tcacttcgca
       61 gcgcatgtgg ccttcaatgc gcgtaatcgg gtcgaccacc aggcggcgtc cggcattatt
      121 gatggtgtat ccctgagttt cgtactgagt gctcatgcct gtttatcctc attgcctggc
      181 tgatgttcgg tttctgtagg ttgctggtta tgacgtctgc gctggtcaac ggcgctggcg
      241 actgcgtgca caccaacagc cgctgccacc acgccaagcg cggttaagcc gacggtatcg
      301 gcggtggaat gagtacccat ttgcggaata tcgaccacgc ggctgtagaa cgaaccgcga
      361 tcccagaaac cattttccgc acagcccagg cagccgtgac cagactggat tgggaaagaa
      421 acgccatcat tccagcgtgt ggaggaacag gcgttatagg tggtaggccc tttgcagccc
      481 attttgtaca ggcagtaacc tttgcgggca gcgtcatcgt cccaactctg gacgaactct
      541 ccggcgtcga agtgggcgcg gcgatagcat ttatcgtgga ttcgctgacc atagaacatc
      601 aacggacggc ccattctgtc gacatctggc aagcgatcaa aggtcaccat gtaagtaatg
      661 atggcgctca tcacatccgg gatcggcggg cagccaggta ctttgataat aggtttgtcg
      721 gtgatgactt tgtcgatagg cgttgcctgc gtcggattgg gtcgcgcggc ctgcacgcag
      781 ccccaggacg cgcaggttcc ccaggcgata atcgcactgg ctccggcagc ggcacgtttg
      841 agtttctcaa taaacggtcg tccgctgctg atacagaaca tcccctgctc gcccagcggc
      901 ggattacctt ctactgcgag gatatatttg ccattgtatt gcgtgatgat gtcttcaaag
      961 acttcttccg cctgggttcc ggcggcagcc atcaaagtat cgtcgtaatc gagggaaatc
     1021 agggaaagga tgacgtcctt tgccagcggg tgagcggagc ggataaaaga ttcggtacag
     1081 caggtgcatt ccagaccgtg gatccatacc accggaatgc gcggtttgtt ctccagcgcc
     1141 caggcaatct ttggtgccat tcccgcgcct aatcccagcg acgtggcagc cagactgcaa
     1201 tatttgagaa agctgcgccg ggtaacgccc tgacgccgca tggcctggta aaatgtttcc
     1261 tcgttattca tatcgcacgt ctcttctcct tgcgacaccg gcaggacacc gggcgcttat
     1321 ggtcggcaag cgacgtccct gtagcaaggc aaaccatcgc ttcacgtgag atgctgaaaa
     1381 cgaaagctca tccttctgca ctggcgcacg tcgccagaaa gtattgttaa taaagcgtag
     1441 tgaaactttt gcacgaaaca atacaaactg tgtggattta tcttttagcg ataaaaatgg
     1501 acctattttt ctttttgccg ggcagtgggg atgtttagcc ggttgccaaa cgagtaaaag
     1561 agaaggaatt catggtgatg aaatgatcaa tctgctgtta tttcacgcta tcaatgcact
     1621 tttggctgtt tttcaggcaa acaaacaaat taggggttta cacgccgcat cgggatgttt
     1681 atagtgcgcg tcattccgga agtgtggccg agcggttgaa ggcaccggtc ttgaaaaccg
     1741 gcgacccgaa agggttccag agttcgaatc tctgcgcttc cgccaaataa gataaggggt
     1801 tagctaaatg ctaacccctt tttcttttgc ctgtcgaaat tctcagggcg ttatatttgc
     1861 ttaatgacct gataatccgc tgttaaacct gttccagatc aaatgcgtaa agatgggtaa
     1921 aacttctggg tgcccttacg cattatcatt atgctgctta attaattaca tctgtcatag
     1981 agagtgactc aatggatcgt attgttagtt cttcacatga ccgtacatca ctgcttagca
     2041 cccataaggt gctgcgtaat acctattttc tgctgagcct gacgctggcc ttttcggcga
     2101 ttaccgcaac tgccagtacg gtgctgatgc tgccatctcc gggtctgatt ctgacgctgg
     2161 tgggtatgta tggtttgatg ttcctgacct ataaaacggc gaataagccg accgggatta
     2221 tctccgcatt cgcctttacc ggttttctgg gatatatcct cggaccaatt ctgaacacct
     2281 acctgtctgc cggaatgggt gacgtaatcg ctatggcact gggcggaacg gcgttagtgt
     2341 tcttctgctg ctctgcgtat gtgctgacca cccgcaaaga tatgtcgttc cttggcggta
     2401 tgctgatggc gggtattgtg gtggtgctga ttggtatggt tgctaatatc ttcctgcagc
     2461 tgcctgccct gcatctggcg atcagcgcgg tcttcattct gatctcctct ggcgctattt
     2521 tgtttgaaac cagcaacatc attcatggcg gtgaaacgaa ctatattcgt gccacggtta
     2581 gcctgtatgt ttcgctgtac aacatcttcg tcagcctgct gagcattctg ggcttcgcca
     2641 gccgcgatta atctcacccg ctaacacttt catagcctcg ctttatgcgg ggctttgttt
     2701 tttgttacac tgccgccagt ctgattaagg tagtaaagaa ttatgctgat cttcgaaggt
     2761 aaagagatag aaacggatac cgaaggctat ctcaaagaaa gcagccagtg gagtgagcca
     2821 ctggcggtgg tgattgcaga gaacgaaggg attgcgctgt cgccagaaca ctgggaagtg
     2881 gtgcgttttg tgcgtgattt ctatctggaa ttcaatactt ctccggcgat tcgtatgctg
     2941 gtaaaagcga tggcgaataa atttggcgaa gaaaaaggta acagccgcta tttataccga
     3001 ctgtttccga aaggtccggc taagcaagcc accaaaattg ctggcctgcc aaaaccggta
     3061 aaatgtattt aatggatacg aaaatcggtt aattcccccg agggatgatg cggctcgctg
     3121 agcacccgtt ctacacgcgc agaacgcggg ccgccacttt ttaaccactg cattaatttt
     3181 tctacctgcc cttcttcacc gcaggcaacc acttccacgc tgccatcgtc aagatttttg
     3241 gcgtacccgg ttagtcccag ttttttcgct tcgtactgcg tggtgtagcg gaatcctacg
     3301 ccctgaactc gcccgtaaac ccaggcaatt atgcagactt tcgacattgc tgctctcctt
     3361 atccttaatg gacgcgttgc aaatccatgg aaaactccgg acaatgacgc ccatttcttt
     3421 gcatcgacag aaaagtaaat tatgagtgta cgtttagtgt tagccaaagg gcgcgaaaaa
     3481 tcattacttc gtcgccatcc gtggatcttt tccggggccg ttgcccgcat ggaaggtaaa
     3541 gccagcctcg gtgaaaccat cgatattgtt gatcatcagg gaaaatggtt agcacgcggc
     3601 gcttattcgc cagcttcgca aatccgggcg cgcgtctgga cgtttgaccc gtctgagtct
     3661 atcgacattg ctttttttac ccgacgttta caacaagcac aaaaatggcg tgactggctg
     3721 gcgcaaaaag atggcctcaa cagctatcgt ttaatcgccg gagaatctga tggcctgccg
     3781 ggtattacta tcgatcgttt cggtaatttt ctggtgctgc aactgctgag tgctggcgca
     3841 gagtatcagc gcgcggcatt aattagtgcc ctgcaaacgc tgtacccgga atgtgcgatt
     3901 tacgatcgca gcgatgttgc ggtacgtaaa aaagaaggga tggagctgac ccagggcccc
     3961 gtcaccggcg agttgccgcc tgccctgctg ccgattgaag aacacggcat gaagctgctg
     4021 gtggacatac agcacggaca caaaacgggc tactacctgg atcagcgtga tagccgcctg
     4081 gctacccgcc gctacgttga aaataaacgc gtactgaact gtttctccta taccggtggt
     4141 ttcgccgtat cggcactgat gggcggttgc agccaggttg tcagcgttga tacctcccag
     4201 gaagccctgg atattgcacg gcagaacgtt gagctgaaca aactggatct gagcaaggct
     4261 gagtttgtcc gtgatgatgt ctttaaattg ctgcgtacct atcgcgatcg cggtgaaaaa
     4321 tttgacgtta tcgtgatgga cccgccgaag tttgttgaga ataaaagcca gttgatgggc
     4381 gcgtgtcgtg gctataaaga catcaacatg ctggcgattc agttgctgaa tgaaggcgga
     4441 gttctcctga ctttctcctg ttctggcctg atgaccagcg atttatttca gaaaatcatc
     4501 gcagatgccg caattgatgc cggtcgtgat gtacaattta tagagcagtt ccgtcaggca
     4561 gccgatcatc cggtgatcgc tacctatccg gaagggctat atctgaaagg gtttgcctgt
     4621 cgcgtcatgt aacttgaaat gtggaatttt acccttatat caaaggtaaa gatttcccgg
     4681 gaggtgacta tgattgccag caaattcggt atcggccagc aggtccgcca ttccctgtta
     4741 ggttatctcg gagtggtcgt ggatatcgac ccggtttatt cgctctctga accgtcgcct
     4801 gatgagctgg cggtgaatga cgagcttcgc gccgctccgt ggtatcacgt ggtgatggag
     4861 gacgataacg gcctaccggt tcatacctac ctggctgaag cccagctaag tagcgaattg
     4921 caggatgaac atcctgaaca gccatccatg gatgagctgg cgcagaccat ccgcaaacaa
     4981 ctccaggccc cgcgtctgcg taactaatcc ctgaacaaat ccccggaagg tttttgcctt
     5041 ccggggattt tttatttggc caggcccagg cgagggatct caatagccgg gcagcgatcc
     5101 atcactacat ttaacccggc atcccgtgcc agtactgccg cttgctcatt aatcaccccc
     5161 agttgcatcc agagggtttt cgcgccaatg gcaatagctt cctgcgctac gccccacgcc
     5221 gcttctgaat tccggaaaac atcgaccata tcgacttttt caggaacttc tgccagcgtg
     5281 ccatacccct gctggcccag cagtgttttg ccggcaactt ttggcgaaac cggaatgacg
     5341 tgatacccct ggtcgagtag atatttcatc acgcgatagc tgggacgatc gggtttgtcg
     5401 cttgccccca ccagcgcaat ggtgtgagtg gacgttaaaa tgcctgcaat atcggtttct
     5461 ttcattattt cctccagact gtttaacaaa gtgtacgaca aagccgacat gccaaccatg
     5521 cagctaatac gaatgaatga gagcaaaagc gggaaatatg tctatatgtt agtaagtatg
     5581 attaaagaaa aattttgaca cattattatt cgggcggtct tttgacagga gctacctatg
     5641 aaaaccggca tcgtgaccac cttgattgca ttatgtttgc cggtatccgt ttttgccacc
     5701 acgctgcggc tttcaaccga tgtcgatctt ctggtactcg acggtaaaaa ggtttccagc
     5761 tctctgctgc gcggtgcgga cagcattgaa ctggataacg gaccgcatca gttagtgttt
     5821 cgcgttgaga agacgattca tctttccaat agtgaagaac ggctgtatat ctccccaccg
     5881 ctggtggtca gttttaatac ccagctcatc aaccaggtca attttcgcct gcctcgtctg
     5941 gagaatgagc gggaagctaa ccattttgat gccgcgccgc gccttgaatt gttggatggc
     6001 gatgcgacgc cgattccggt aaagctggat attctcgcca ttacctcaac ggcaaaaacg
     6061 attgattatg aggtcgaggt tgaacgctat aacaaatccg cgaaacgcgc ttcactaccg
     6121 caatttgcca cgatgatggc agatgacagt acgctgcttt cgggtgtttc cgagctggat
     6181 gctattccac cgcagtctca ggtgctcaca gaacaacggc tgaagtattg gtttaaactg
     6241 gctgacccac aaacgcgaaa tactttcctg caatgggcgg aaaaacaacc atcttcctga
     6301 gatttttgtg cctgtgcgca ggctttttcg gtctttatct tgcagcgata agtgcttaca
     6361 gtaatctgta ggaaagttaa ctacggatgt aacattatgg aactgacgac acgcacttta
     6421 cctgcgcgga aacatattgc gctggtggca cacgatcact gcaaacaaat gctgatgagc
     6481 tgggtggaac gacatcaacc gttactggaa caacacgtac tgtatgcaac aggcactacc
     6541 ggtaacttga tttcccgtgc aaccggcatg aacgtcaacg cgatgttgag tggcccaatg
     6601 gggggtgacc agcaggttgg cgcattgatc tcagaaggga aaattgatgt attgattttc
     6661 ttctgggacc cactaaatgc cgttccgcac gatcctgacg tgaaagcctt gctgcgtctg
     6721 gcgacggtat ggaacattcc ggttgccacc aacgtggcaa cggcagactt cattatccag
     6781 tcgccgcatt tcaacgacgc ggtcgatatt ctgatccccg attatcagcg ttatctcgcg
     6841 gaccgtctga agtaatattg cacaggtggc aaacgccacc tgtttcttac ggttttctcg
     6901 ccaccggcac atccagattt ttcagtattt ccacaaaggg agagggattc tctttgttaa
     6961 acagtgccca tacccgatgg cgtgcccggg tcagcgccac gtacattaac cgccgttctt
     7021 cagcgtccgg gaaatcctca accggtggca gtagcgcctc ttccataatc gactcccgcg
     7081 ccgcagccgg aaaaccatca ctcccctcct gcaagccaac gatgatgacg taatccgcct
     7141 gttgcccttt gctggcatga atggtcataa agtcgatttg caacttcggc cagcgtgttg
     7201 ccgctttttc caggctggca ggcctcatgt gatggtaacg cgccaggatc agaatgcgct
     7261 cttccggttt ggcataacca gagagcttat ccagcaaagc gtcaagttga ctctcatcca
     7321 ataacgtgac ggcttttttg tctccattgg ttaagctgtt tagcggcttt ttcagctggc
     7381 ctgggttctg ctgaataaac cggtttgcca cctcaccgat acgactgtta aaacggtaag
     7441 tcgtgtctaa atcgcagcga tcaccttcac caaagttttc atggaaagcg gtggtgagcg
     7501 acatttgcgc accgctgaag cgataaatcg cctgccagtc atcaccaacg gcgaacaacg
     7561 tcgtctgact gttttgcttg cgtaatgccg ctaacaacgc tgcgcgctgc ggcgagatat
     7621 cctgaaattc atcaaccaga atatgcttcc acgggctgat aaagcgacct ttctccagaa
     7681 tcacaatcgc ctggtgaata aggcccgaaa aatcgacagc gttttctgcc ttcagcgcac
     7741 ctttccaggc ttttaataac ggagccatca acttgatacg tttactgaac agatcgcgaa
     7801 tctcttcggg tgcactggca atcatttctg cctgtgcacc accgtgcatc cgcatcagac
     7861 ttacccaacg atcgaggcgt gaggcaagac gacgctgtaa tttttcatca tcccagaagt
     7921 taccttctgg cactgaccac tgcatttctt ccgtcagcca ttgccgccag cccttcgcct
     7981 gcgctttctt ttcgctgcat tgcttgcgcc actcagcaat aaaaagttca tgacgggcag
     8041 cggtatcatt ttccagtttg ctgactatcg gaactttttt gctgccctgc tgaataatat
     8101 gcagcgccag cgcatgaaat gtgcgtgcgg taatgtcttc ggtatgtagc cgttcgcgaa
     8161 tacgctcgtc catctcttca gcggctttgc gaccaaacgc cagcaataaa atttgctcag
     8221 gggacgcttc accacgtgcc agcaaccagc ctgcacgggc caccagcacc gacgtttttc
     8281 cgcttcctgc accagccagc actaacaaag aatgctcgcc attaacgact gcccgggcct
     8341 gcgccggatt cagcggtgaa gactcgacct ggcggaaaaa atccgcatac tcggtaagca
     8401 tggcttcggt atacgcctga ttatgctgca accgagcgcc ttcaatgtcc ttcaaccagg
     8461 cctgacattt acgccacgcc tcacggcagt tatcgaattc ttccagtcgg ttaaccggca
     8521 acggcaacgc cgacaaagcc tggcggattt gttgctgcac accagaggtt tgctcacgcg
     8581 tcagccattt attttctccg gtgcgcgtgg caatcaaatc cagttgctga cgtaaaacgc
     8641 cagacgcaat ttcgctcatc tccccactcc accgccgcca gtgagtatca agatgatggt
     8701 aaaaacgctg cgtctcgccc cattcggtgc cgtgcagacg caccactttt tcgtccggca
     8761 gtacaaattc cagctcgccc cataccagac cgcgcttaca gtgaatcgcc agtaattgat
     8821 taaaaggaat aaggtattcg tggcgatcgc cagagacttt aatcccggca ttgaggatca
     8881 ccgcccgatc gtaagggtgc tgtgccagac gttttccaag cgttgtcgct ttcagttcca
     8941 tgactctgta gatccacacg aaatgatttc ttatcagtgt aaccgtcaga gaaaacgtgc
     9001 tccagcccaa aaaaacgtta caattgccgc ccattactga aaaccacagt aaagcgaggt
     9061 tttatgcgta ccgttttgaa cattctgaac tttgtgcttg gcggatttgc caccactctg
     9121 ggctggctgt tggcgactct ggtcagtatt gtgctgattt ttaccttacc gctgacacga
     9181 tcctgctggg agatcactaa actgtctctg gtgccttatg gcaatgaagc tattcatgtc
     9241 gatgaactga acccggctgg caaaaatgtg ctgctaaata ctggcggtac ggtattgaat
     9301 attttctggc tgattttctt tggctggtgg ttatgcctga tgcacattgc aacgggcatc
     9361 gcacaatgta tttccatcat tggcattcct gtcggcattg cgaactttaa aattgccgct
     9421 attgcactat ggccggtcgg tcgtcgcgtg gtatcggtag aaacagcgca agctgcgcgt
     9481 gaagccaatg cacgtcgtcg ttttgaataa tccactaata tggcctttat gctaagtcct
     9541 ttgctcaaac gctatacctg gaacagcgcc tggctgtatt acgcgcgtat ttttattgcg
     9601 ctttgtggaa ccacagcgtt tccgtggtgg ctgggtgatg taaaactgac gattccgctg
     9661 acgctgggga tggtggcagc ggcgctgacc gatctcgatg accgactggc gggacgtttg
     9721 cgtaacctca tcattacgct gttctgcttt tttatcgcct cggcctcagt agaactgctg
     9781 tttccctggc cctggctatt tgcgattggc ttaacgctct caaccagcgg cttcattttg
     9841 ctcggcggtc tgggtcaacg ctatgcaaca attgccttcg gtgcgttact gatcgccatt
     9901 tacactatgt tggggacgtc actgtatgag cactggtatc agcagccgat gtatctgctg
     9961 gccggtgccg tctggtacaa cgtcctgaca cttattggtc atctactgtt cccggtccgc
    10021 ccgctgcagg acaacctggc gcgttgctat gaacaactgg cgcgttatct tgagctcaag
//
LOCUS       DAMZFT010000171         9950 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.171, whole genome shotgun sequence.
ACCESSION   DAMZFT010000171 DAMZFT010000000
VERSION     DAMZFT010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9950)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9950)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9950
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.171"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            18..1433
                     /locus_tag="PN977_004465"
     CDS             18..1433
                     /locus_tag="PN977_004465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001420784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HDI6044418.1"
                     /translation="MAALAVATNFYGGVIELTGSDAFKQKAMQLIVEYDIKVRMKLPA
                     QRAALEKLRKEKEMVHDAIVTHRPTPELNRQSPEETTMAQPEPPVSPKAEAEQTAGCT
                     AETPHPEAPVTAKAEAPRADRPEQPAAPSSAPEKAPVSAQPQQPDTPEEEPPGKLSPG
                     ESVTAVLTNYGEKEYAPGKGRCFFVELTNRSGKREYWGKGLEELVKNHQKGDPVTLTL
                     QARERSPSSQGKPEWVRNIWAMTPVSNGITVDNDLPHAGQHILEFPMKTFSQVMSQMQ
                     QHWPQIMQEVRSPKNPPNNLYIGEDRQPASEPQDQSMRLPLAGKAPDELIPAVASADK
                     DNRQLNLLLVHSADEHLQGVVRLNGTLYPALATPTADNRQLVINALTDNGLQFAGYGE
                     AVNHDENTHQRPAPQMMQFHLKQQDSPLFAAIHKPEEQPDKLFRSLGFEQTWKEWSDS
                     QKAEDRQEKTLQQAQSHSPGR"
     gene            1450..1800
                     /locus_tag="PN977_004466"
     CDS             1450..1800
                     /locus_tag="PN977_004466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HDI6044419.1"
                     /translation="MNMKKTLLVILTGITLASPVAGAATCRAGAAAEAGSRAAWERAR
                     TASQAWSQRENDVASSLQSCLSRIKDVSITLPQFPSLDKLLRDLENQVCDAVVDRVNE
                     HLPGNIDPWKDYNL"
     gene            1812..2507
                     /locus_tag="PN977_004467"
     CDS             1812..2507
                     /locus_tag="PN977_004467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DotI/IcmL family type IV secretion protein"
                     /protein_id="HDI6044420.1"
                     /translation="MSEAQPTSPVSPEADNSGDIYNPVLRTIAQSEQRAALVPGLVRA
                     LLCSSAALVISLTGNIIQYWHATDVTREYFATDNGRLVRLAPTSQPAWSQSDVMTFGS
                     MTLSEAFNLDFVHYRKQITTLAPRFSEAGFAGYVNALQASNILDTIKKEKFNLTATIG
                     AGTLVNQGQMENGIWFWKFQYPVRMRLVGQTSTRPEQAFTFEITVRRVDPRIKPGGME
                     ISQMVSRNAAAGI"
     gene            2518..3492
                     /locus_tag="PN977_004468"
     CDS             2518..3492
                     /locus_tag="PN977_004468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001191879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraN"
                     /protein_id="HDI6044421.1"
                     /translation="MQIHHLLGALLIVCGTVTAADNPGWQNARTPQAGGKTGQTPGSA
                     PAGGPPPATQLTGPLPPPASPLVQEAASLDSPLNAGEIRELRGRMADNERAISAPVTS
                     VVPRISSLTVNLSPGASLPLVRTALHNVSVVTFTDINGSPWPQSDPPYNAAPGHFDVQ
                     FNGNMVSVTPLRAWAAGNVTVYLKGLSVPVILNITSGETDTNTLSQEIDSRLDLRIPR
                     QGPASPVVAVPSEKIALHDATLQAFLDGVPPDDPTVRRLKFSGNVPDTTIWQHGDDLL
                     VRSRAMLRDEFEQTLASADGTRLWKLPVTPLLTFSVNGQSIHVTPELE"
     gene            3496..4827
                     /gene="traO"
                     /locus_tag="PN977_004469"
     CDS             3496..4827
                     /gene="traO"
                     /locus_tag="PN977_004469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001272008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraO"
                     /protein_id="HDI6044422.1"
                     /translation="MSAEQDTAAKSGKKLSALLVLGGVIIIGGGYLLLTWLSPENSAS
                     LSSVNINSTTTGSGRTVVESQHYRDLLRADNERGAAEALRNNQTFIASLPRGLDTDHG
                     KNQSRDTQPADQAEGSGQPSTPPQADQTAREKQREALQKLLARINTQHPDARPPVVAA
                     AMWGATGAPGISSHASPPGTGTSPQPRLQEASLATPAARTGIQLIPALTRVPAFIDTE
                     VDSDNAVSRVVASVPAGPWAGAVLYSQDARLAGKGMDIHFDRMMWRGMFLKVNAYALN
                     ERTGMSTVASDVNHRWFKHIVLPSILGGAGKGGSLYKDANTQILQGNYGSLTGRVGMP
                     DGRALAGVMAGGMAERGSELLTRQAESEPYRQVTVSRGEVVSILFVEPVMSNDISPTS
                     SGTAPSVPPPSGRPLTQEEAERQASDRIQAAVMRKQNEMRQRYGIQQESHP"
     gene            4824..5537
                     /gene="traP"
                     /locus_tag="PN977_004470"
     CDS             4824..5537
                     /gene="traP"
                     /locus_tag="PN977_004470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000787010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraP"
                     /protein_id="HDI6044423.1"
                     /translation="MKNEHEDPGSFGETTTPASGKSFLKRQYMGYSLPVWAGCVLIIL
                     AAVWYKFLRTPSEPPADAGFAVTPPAAESWAEPMPITETGSGNTMSAPPVTPQADMQQ
                     LARDLKAELDRRDEEIRRTLTMLKESITTLGDTIRKDEAWAQETRQLLDETRKQVHAL
                     QMQGPLQIPTGNNGQSTKTPPSRLQGMQIMSVEKGMAWIRWQGSAWAVREGDRLGNIT
                     ITRIEPSSRSVITSAGTLR"
     gene            5537..6064
                     /gene="traQ"
                     /locus_tag="PN977_004471"
     CDS             5537..6064
                     /gene="traQ"
                     /locus_tag="PN977_004471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001055571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraQ"
                     /protein_id="HDI6044424.1"
                     /translation="MSIDALTAIENFASGIFSAGMEFLFTWGELLGIIGLIGHLMRAR
                     AEGRHSMGPGKFIAGIVICGMLVALPSFINAGGTQMGFRADSFGPIAYVQPTTFGAAA
                     GAANAMLSLVKLAGVGFAMNGISIWRKSLLDGHTALSSSESISKGNVKFVAGVLLVFI
                     DRVIDASLASLGLAF"
     gene            6111..6509
                     /locus_tag="PN977_004472"
     CDS             6111..6509
                     /locus_tag="PN977_004472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001605438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraR"
                     /protein_id="HDI6044425.1"
                     /translation="MSRFRAMAARLCAAFIYVQLCLVACRDRLIRLLLLLPVILLPHT
                     AMANTLFDMIKTVGDGAKGSSSSFVTIAQVIGLVLFIAGLLGLKQIGKKPGAGLACIL
                     SIVVGACLAVVPEMMSSGQKTLGLSPVSIS"
     gene            6566..6817
                     /locus_tag="PN977_004473"
     CDS             6566..6817
                     /locus_tag="PN977_004473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003665769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraS"
                     /protein_id="HDI6044426.1"
                     /translation="MSCELPPFTLLRTEHGNVIARDNRDNQEFVFAECYSTAIAALIL
                     AAVTLYTERKTYEQNTATHRPGPGTLAFPAPATGHGIQR"
     gene            6732..7550
                     /locus_tag="PN977_004474"
     CDS             6732..7550
                     /locus_tag="PN977_004474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001353682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraT"
                     /protein_id="HDI6044427.1"
                     /translation="MNKTPPLIAPARERWLFPLPQPDTVFSGESLLPPPVLSTPGRCP
                     CCRRTVTHRFILEDSWPLQQMADTCRDTVVLLEKNLTRVMRLKKHPVPENADEKKKHT
                     RTLQDAERSLAQARLSARRLALRHVEKSQIVTTDALSENESELLQPEGPPFHLCAFCH
                     AWHCLNGYAAAQGVMVWLPDLHPASVVALNARALKEIFSDERKRVRQGRAVLNALVQN
                     RLAVEEKFRTWRPADFADALRRWPPAQRQMLREKMDGVALILLPDSFPDKKYVM"
     gene            7607..7966
                     /locus_tag="PN977_004475"
     CDS             7607..7966
                     /locus_tag="PN977_004475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000380400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6044428.1"
                     /translation="MDTLSAAYQFTADAFRHFIIRTLTDDIPPDGVEKEIARFILRHP
                     AFNGGWLLFLAAEIHEKSRTHDCITVTVSVDNYLSWMDIAVSGLPELPDMPFCDFFIR
                     RQQFIYPGVPPTKSGEE"
     gene            7966..8346
                     /locus_tag="PN977_004476"
     CDS             7966..8346
                     /locus_tag="PN977_004476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000690882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6044429.1"
                     /translation="MKIHRISPETLITLILAHLAGKADSTAKEEHRLLRRFLRDDDGR
                     LAGILLNIAGILQFNRELSARHNYPATPLTEFSLRKRGKQLHLCLCSLRFFYIPPVFI
                     RNERRKCIVVHINKITYPPVNSLR"
     gene            8522..>9950
                     /locus_tag="PN977_004477"
     CDS             8522..>9950
                     /locus_tag="PN977_004477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDI6044430.1"
                     /translation="MNINRIIFAIEDCISTLSRYSVSAFFPDYCDLHTVIGLDQQDRE
                     RRPWLKAPYIATTKQGYYLSVFEISGALKEMDENPDLTAPGTLESLITALAERMNTAY
                     KNIGHKISFVFERDPERGRDEIEAMLLPQRKSIARTGILLQDVLDEKVTTLTPWLVRE
                     RCWLTVWSSEELLSRTDRKDHQTRIRKLAEHAPPARFAQDPWRWTLSALKIRHDAFLD
                     TLEQALTHDSDGLLVRLMDIHEVGREIRRQLERNSTPAVWQPHLPEDARPAGWRQGED
                     TSVFHAPSLNLQLFTTQPETHGSLIKAGDLWHGMVAITLPPQNLRTFNQLVRDVPRAI
                     PWRIRMDLMPGGMKALGVKKTLLTYSSFISPLRPMYDSVMMLNDINKHDPVCIMTIVA
                     TTWGNSREVCTRNQALLKSALEGWGVCGTTTTFGDPRRAWVNTVLAASHSSGPIPLYP
                     PLSHALSLFPLNRAGSVWRGQGNLMM"
BASE COUNT         2363 a         3002 c         2605 g         1980 t
ORIGIN      
        1 cggatgacaa aaaggttctg gctgcccttg ctgtggccac caacttctat ggcggcgtta
       61 tcgagctgac cggcagtgat gcctttaaac aaaaagccat gcaactgatt gtcgaatacg
      121 acatcaaggt tcgcatgaaa ctcccggccc agcgggcagc actggaaaaa ctccgcaagg
      181 aaaaagagat ggtgcatgat gccattgtca cccacagacc cacgccggaa ctcaaccgcc
      241 agtcaccgga agaaacaaca atggcacaac cggaaccccc tgtgtcaccg aaggcagagg
      301 ctgaacaaac agcaggctgt acggctgaaa caccgcatcc ggaagcgccg gtcacggcaa
      361 aagctgaagc cccccgggcc gacagaccgg aacaaccagc cgccccgtcg tcagcgccag
      421 aaaaagcccc tgtcagtgca cagccgcagc aacccgacac accggaggaa gagccccccg
      481 gcaagctcag ccccggtgaa agtgtgactg ccgtcctgac aaactacgga gaaaaagagt
      541 atgcgcccgg aaaagggcgc tgcttttttg tggagctcac caaccgcagt ggcaaacggg
      601 aatactgggg aaaaggcctg gaagaactgg tcaaaaacca ccagaaaggc gaccccgtca
      661 cgctgacatt gcaggcccgg gagagatcac cttcatcaca gggcaaaccg gaatgggtgc
      721 gaaatatctg ggccatgact cctgtcagta acggcattac cgtcgataat gaccttcctc
      781 atgctggtca gcatatcctg gaatttccga tgaaaacatt cagtcaggta atgtcacaaa
      841 tgcagcaaca ctggccacag ataatgcagg aagttcgttc ccccaaaaat cctcccaata
      901 atctgtacat tggtgaggac agacaaccgg catcagaacc tcaggatcaa tcaatgcgac
      961 tccccctcgc tggtaaagcc cccgatgaac tgattcctgc cgtcgcctcc gccgataagg
     1021 acaatcgtca gttgaatctt ctgctggtcc acagcgcaga tgagcatctg cagggagtgg
     1081 tccgtctgaa cggcaccctc tacccggcac tggccacccc gacagcggac aaccgtcagc
     1141 tggtcatcaa tgcactgacg gataacggtc tgcagtttgc cggctacggt gaagctgtca
     1201 accacgatga gaatactcat cagcgtccgg ccccacaaat gatgcagttt cacctgaagc
     1261 aacaggactc cccgttgttt gccgccatcc acaaaccgga agagcagccg gacaagcttt
     1321 tccgctctct gggttttgag cagacctgga aagagtggtc tgacagccaa aaggcggagg
     1381 acaggcagga aaaaactctg caacaggcac aatctcacag ccccggccga tgagccggta
     1441 aggacgacca tgaacatgaa aaaaacgctg ctggtcatac tgaccggcat caccctggca
     1501 tcaccggtgg caggtgccgc cacctgccgg gcgggggccg ctgccgaagc cggcagcaga
     1561 gccgcctggg agcgggcgcg cacagccagc caggcatggt cacagcggga gaatgacgtc
     1621 gcctcttccc tgcagtcctg cctgtcccgc atcaaggacg tcagcatcac tcttccgcag
     1681 ttccccagcc tggacaaact cctccgcgac ctggaaaacc aggtctgtga tgccgtcgtg
     1741 gatcgggtga atgaacatct gccggggaat atcgacccct ggaaagacta taacctctga
     1801 acaggagcac catgagcgaa gcacaaccca cctcccctgt ctcaccggaa gcggataaca
     1861 gcggagatat ttataacccc gtgctcagaa ccatcgcaca gtcagaacaa cgggccgcac
     1921 tggttcccgg actggtcagg gccttgctgt gtagcagcgc agctctcgtc atcagtctga
     1981 ccggcaatat catacagtac tggcatgcca ccgacgtgac gcgcgaatac tttgccaccg
     2041 acaatggccg gctggtccgc ctggccccca ccagtcagcc agcctggagt cagagcgacg
     2101 tcatgacgtt tggcagcatg acgctgagcg aagcttttaa tctggatttt gtccactaca
     2161 ggaaacaaat cacgacactg gctccacgct tttccgaagc cggttttgcc ggctatgtga
     2221 atgctcttca ggcctccaac attctggaca ccattaaaaa ggagaaattt aacctcacag
     2281 ccaccatcgg ggccggcacg ctggtgaatc agggccagat ggaaaacggc atctggttct
     2341 ggaaattcca gtatccggtc aggatgcgtc tggtcggtca gaccagcacc cgccccgagc
     2401 aggccttcac ctttgaaatc actgtccggc gcgtggaccc acgaatcaaa cccggcggca
     2461 tggaaatcag ccagatggtt tcccgtaacg ccgcagcagg aatctgagac tcatatcatg
     2521 caaatacatc atcttcttgg cgcactgctt attgtgtgcg gcactgtcac ggctgcagat
     2581 aacccgggct ggcagaacgc acgaacccca caggctggcg gaaagacagg acagactccc
     2641 gggagcgccc ccgcaggcgg ccccccgcca gccacacaac tcacaggccc cctgccgcca
     2701 cctgcctctc ctcttgttca ggaagccgca tcactggatt cccccctgaa tgcaggtgaa
     2761 attcgggaac tgcggggacg aatggccgat aacgaacggg ccatcagcgc acctgtcacc
     2821 agtgtggtgc cccgtatcag ctccctcacg gtaaatctgt cacccggggc cagtctgccg
     2881 cttgtccgaa ccgcactgca taacgtcagc gtggtaacgt tcacggacat caacggctcc
     2941 ccgtggcctc agtcggatcc gccttataac gcggcacccg gtcactttga tgtgcagttc
     3001 aacggcaata tggtctccgt cacccccctg cgcgcctggg ctgcagggaa tgtaacggtc
     3061 tacctcaaag gactgtctgt accggtcatt ctgaatatca ccagtggtga aaccgacacc
     3121 aataccctga gtcaggaaat cgacagccgt ctggatctgc gcattccccg tcagggacct
     3181 gccagtcctg tggtagccgt accatcagag aagattgccc tgcatgatgc caccctgcag
     3241 gcctttctgg acggtgttcc tcccgatgat cccaccgtgc gtcggctgaa attctccgga
     3301 aatgttccgg acaccaccat ctggcagcac ggcgatgatc tgctcgtgcg atcgcgggcc
     3361 atgctgcggg atgaatttga acagacgctg gcatctgctg acggtacgcg tctgtggaaa
     3421 ctgccggtga ctcccttact caccttctcc gtgaatggtc agtccattca cgtcacccct
     3481 gaactggagt aaatcatgag cgcagaacag gacacagccg caaagtccgg caaaaaactg
     3541 tcagccctgc tggttctcgg gggcgtgatt atcatcgggg gcggatacct gctgctcacc
     3601 tggctttccc ccgaaaacag cgcatcgctc tcgtcagtca atatcaacag cacgacaaca
     3661 ggcagcgggc ggaccgtggt ggagtcacag cactaccggg atttactgcg tgctgacaat
     3721 gagcgcgggg ccgcagaagc attacgtaat aaccagacgt tcatcgccag tctgcccagg
     3781 ggactggata ctgatcacgg aaagaatcaa agtagggata ctcagcctgc tgaccaggcc
     3841 gagggttctg gtcaaccatc aacgccccct caggcagatc aaacagcccg ggaaaaacag
     3901 cgggaagcgt tacaaaaact gcttgcccgc atcaacacac agcatcctga cgcccgcccc
     3961 ccggtagtgg cggctgcaat gtggggtgcc actggtgctc ccgggataag ctcacacgcc
     4021 tcaccacccg gtaccggcac atcaccacaa ccacgtctgc aggaggccag tctggcaacc
     4081 ccggcagctc gtacagggat acagctgata ccggcactga cccgcgtacc tgccttcatt
     4141 gatacggaag tggactctga caatgctgtc tcccgcgtgg tggcctccgt acctgccggc
     4201 ccatgggccg gggccgtcct ctacagccag gacgccagac tcgccggtaa gggaatggat
     4261 atccactttg accggatgat gtggcggggc atgtttctga aggtcaatgc atatgccctg
     4321 aatgaacgaa caggaatgtc aaccgtggcc agcgacgtga accaccgctg gtttaagcac
     4381 attgttctgc cgtccatact tggcggtgcg ggaaagggag ggagtcttta caaagacgcc
     4441 aacacacaga tccttcaggg aaactacggc agtcttaccg gcagggtggg aatgcctgat
     4501 ggcagggcgc tggccggcgt catggcaggc ggcatggccg aacggggcag cgagctgctg
     4561 acccgccagg cagagagcga accttaccgt caggtgacag tatcacgggg cgaggtcgtc
     4621 tcaattctgt ttgttgagcc ggtcatgagc aacgatatca gtcccacctc ctccggcact
     4681 gccccttctg tgccaccgcc ctccggacgc ccgctcaccc aggaagaagc agagcgtcag
     4741 gcgtctgaca ggatccaggc agctgtcatg cgtaaacaga acgaaatgcg gcaacgttac
     4801 ggaatacaac aggaaagtca tccatgaaaa acgaacatga agatcccggc agttttggcg
     4861 aaacaacgac accggcatcc ggcaaatcct tcctgaaacg ccagtatatg ggatacagcc
     4921 tgccagtctg ggccggctgt gtcctcatca tcctggccgc agtctggtac aagtttctgc
     4981 ggacaccctc agagccccct gcagatgcag gcttcgctgt cacaccgccc gcagccgaaa
     5041 gctgggcaga gcccatgccc ataacggaga ccgggagcgg aaacaccatg tccgcgcctc
     5101 ctgtcacccc acaggcagac atgcagcagc ttgcccgtga cctgaaagca gagctggaca
     5161 ggcgggatga ggaaatccga cgtaccctga ccatgctgaa agagagcatc acaacccttg
     5221 gcgacaccat acggaaagat gaggcctggg cacaggaaac ccgtcagttg cttgatgaaa
     5281 cccgtaagca ggtgcatgcc ctgcagatgc agggaccact gcaaatcccg accgggaata
     5341 acggccagtc aacgaaaacc ccgccttccc gcctgcaggg catgcaaatc atgtcggtgg
     5401 aaaaaggaat ggcctggatc cgctggcagg gtagcgcctg ggcagtacgg gaaggtgaca
     5461 ggctggggaa tatcaccatc acccggattg aaccgtccag ccgctcagtc atcacctctg
     5521 ccggtacatt acgatgatga gcattgatgc cctgacagcc atagagaact ttgccagcgg
     5581 tatattcagt gcaggcatgg agtttctctt cacctgggga gagctgctgg ggattatcgg
     5641 cctgattggt catctgatgc gcgcccgggc agaagggcgt cacagcatgg gaccgggaaa
     5701 atttatcgcc ggtatcgtca tctgcggcat gctggtcgcc ctgccatcct tcatcaatgc
     5761 cgggggtacc cagatgggat tcagggcaga ttccttcggc cctatcgctt atgtacaacc
     5821 aaccaccttt ggtgccgctg ccggtgccgc caatgccatg ctctcactgg taaaactggc
     5881 cggagtggga tttgccatga acggcatttc catctggcgt aaatccctgc tggacgggca
     5941 taccgccctg agcagcagtg agtccatcag caaaggaaac gtcaagtttg ttgccggcgt
     6001 tctgctggtt tttatcgatc gcgtcatcga tgcctctctg gcgagtcttg gtctcgcctt
     6061 ctgatccaca ttcattttca gtaattcagt ctgttagtta aggagactat atgtcacgat
     6121 tccgtgccat ggcggcgcgt ctgtgcgctg catttattta tgttcagtta tgcctggtgg
     6181 cctgccgtga ccggctgata cgcctgttac ttctgcttcc cgtgatactg ctgccccaca
     6241 cagcgatggc aaacacccta ttcgatatga tcaaaaccgt tggcgatggt gcaaaaggct
     6301 ccagcagttc atttgtgacc atcgctcagg ttattggcct ggtgctcttt attgccggcc
     6361 tgcttggcct caaacaaatc ggtaaaaaac ccggagcagg gctggcctgc atactgtcca
     6421 ttgtggtcgg tgcctgtctt gccgtcgtac cggaaatgat gagcagcgga cagaaaacgc
     6481 tcggtctttc tccggtctcc atcagttaat ccacaacggc accggtttcc cggtgccgtc
     6541 ttcacacaat gaagaggacc agaccatgtc ctgtgagctg ccccctttca ccctgctcag
     6601 aacagagcac ggcaacgtga ttgcccgcga caaccgggat aatcaggagt ttgtgtttgc
     6661 agagtgttat tccaccgcca tcgcagcact gattctggct gccgtcaccc tgtatacaga
     6721 gagaaaaacg tatgaacaaa acaccgccac tcatcgcccc ggcccgggaa cgctggcttt
     6781 tcccgctccc gcaaccggac acggtattca gcggtgaatc cctgctgcca ccacccgtgc
     6841 tctcaacacc cggacgctgt ccctgctgtc gcaggaccgt cactcaccgc ttcattctgg
     6901 aggattcatg gccattacag cagatggctg acacctgccg ggatacggtc gtcctgctgg
     6961 aaaaaaacct cacccgggtg atgcgcctga aaaaacatcc ggtaccggaa aacgctgacg
     7021 aaaaaaagaa acacacccga accctgcagg atgcggagcg gtccctggca caggcccgcc
     7081 tgtcggccag gcgactggcc ctgcgtcatg tggaaaagtc acaaatcgtc acaacagatg
     7141 cactcagcga aaatgaaagt gaactgctgc agccggaagg cccgcccttt catctctgtg
     7201 ccttctgcca cgcctggcac tgcctgaacg gctacgctgc cgcacagggg gtgatggtct
     7261 ggctccctga tttgcatccg gcctcggtag ttgctctcaa tgcacgggca ctgaaggaaa
     7321 ttttcagcga tgagcgtaag cgagtcagac agggacgggc cgtactgaat gccctggtac
     7381 agaaccgcct ggccgttgag gagaaatttc gcacctggcg tccggcggat tttgctgatg
     7441 cactgcgccg ctggccgccg gcacagcgtc aaatgttgcg ggagaaaatg gacggtgtgg
     7501 cgcttatcct gctgccggat tcgtttcctg acaaaaagta cgtcatgtga cacacaccgg
     7561 tgccggtgac gttctgccgg ccctctcaac ttacagagga acaaaaatgg ataccctctc
     7621 tgccgcatat cagttcaccg ctgatgcatt ccggcacttt attatccgga cactgacgga
     7681 cgatattccg cctgatggag ttgaaaaaga gattgcccgg ttcattctcc gccacccggc
     7741 atttaacggc ggctggctgc tctttctggc tgcagaaatt catgaaaaat cccgtaccca
     7801 tgactgcatc accgtcacag tgagcgtcga taactatctc tcatggatgg atattgccgt
     7861 gtccggtctc ccggaattac cggatatgcc cttctgtgat tttttcattc gccgccagca
     7921 gtttatttat ccgggtgtac ccccgacaaa atcaggagaa gaatgatgaa aattcaccgc
     7981 atatccccgg agacgcttat aacactcatc ctcgcacacc tggccggaaa agctgacagt
     8041 accgccaaag aagagcaccg gctactccgt cggttccttc gggatgatga cggcaggctg
     8101 gccggcatac tgctgaatat tgccggaata cttcagttca acagggaatt aagcgcccgg
     8161 cataattatc cggccacacc cctgactgaa ttttcattaa ggaaacgggg aaaacaactg
     8221 catctctgtc tctgcagtct ccgtttcttt tatatccccc ctgtatttat acgaaatgaa
     8281 agaagaaaat gtattgttgt acatattaat aaaatcactt atccgccagt aaattcacta
     8341 cgctgacagc gtgtttccac cttattacat ttttattatt tactgtaatc acacagaaat
     8401 acgcacctgc ccctcaacag attatttatt ttctctgctg ttttttcatg ccacatcacc
     8461 tgctgtggca tcaaaaaata tcagaccgtg atgattcatc cttaccggag tgctccatgc
     8521 catgaatatc aaccgcatta tttttgccat tgaagactgt atcagtaccc tgtcacggta
     8581 ttccgtttcc gcattcttcc ccgattactg tgatctgcac accgtcatcg gtctggacca
     8641 gcaggaccgc gaacgccgcc cctggctgaa agccccctac attgccacca caaaacaggg
     8701 gtattacctt tccgtctttg aaatcagcgg tgccctgaaa gaaatggatg aaaacccgga
     8761 cctgacagcc cccggcacac tggaatcact gattacagcg ctggcagagc gaatgaacac
     8821 tgcctataaa aacatcggtc acaaaatcag ttttgttttc gaacgcgatc cggagcgtgg
     8881 aagggatgaa atcgaagcca tgctcttacc ccagcgaaaa tccatcgcac gcacggggat
     8941 cctgcttcag gacgttctgg atgaaaaagt caccacgctc accccctggc ttgtccggga
     9001 acgctgctgg ctgaccgtct ggagctctga agaactgctg agccggaccg atcgcaagga
     9061 ccaccagacc cgtatcagga aactcgctga acacgctccg ccagcccgat ttgcgcagga
     9121 tccatggcgc tggacacttt ccgcactcaa aatacggcat gacgcctttc tggacacgct
     9181 ggagcaggcc ctgacacatg attctgatgg tctgctggtt cgcctgatgg atattcatga
     9241 agtgggacgg gaaatccgcc gtcaactgga gcgcaacagc acacctgcag tatggcagcc
     9301 tcatctgcct gaagatgccc gtcctgccgg ctggcgacag ggcgaggata cctctgtatt
     9361 ccacgcaccg tcgctgaatc tgcagttatt caccacacag cctgaaacac acggcagcct
     9421 gataaaggcc ggtgacctct ggcacggtat ggtcgccatt accctgccac cccagaatct
     9481 gcggacattc aaccagctgg tcagggatgt gccccgcgca atcccctggc gtatccggat
     9541 ggatctgatg cccggcggca tgaaggcgct tggagtcaag aaaaccctgc taacctacag
     9601 cagctttatt tcccctctcc ggcccatgta tgactcggtg atgatgctga atgacatcaa
     9661 caaacatgat ccggtctgta tcatgaccat tgttgccacc acatggggaa acagccggga
     9721 agtctgtacc cgtaaccagg ctctcctgaa atctgcgctg gaaggctggg gggtatgcgg
     9781 caccaccacc accttcggtg atccgagacg tgcctgggtg aacacggttc tggcagcctc
     9841 ccacagctcc ggcccgatcc ccctttaccc gcccctttca cacgcccttt ccctgtttcc
     9901 cctgaacagg gcaggctctg tctggcgggg acagggaaat ctgatgatgc
//
LOCUS       DAMZFT010000172         9928 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.172, whole genome shotgun sequence.
ACCESSION   DAMZFT010000172 DAMZFT010000000
VERSION     DAMZFT010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9928)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9928)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9928
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.172"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..139
                     /locus_tag="PN977_004478"
     CDS             <1..139
                     /locus_tag="PN977_004478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000560980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HDI6044431.1"
                     /translation="RAEALYDYFVERCRQQEMNTQTGRFAADMQVSLVNDGPVTFWLQ
                     V"
     gene            184..1125
                     /gene="fabY"
                     /locus_tag="PN977_004479"
                     /gene_synonym="yiiD"
     CDS             184..1125
                     /gene="fabY"
                     /locus_tag="PN977_004479"
                     /gene_synonym="yiiD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid biosynthesis protein FabY"
                     /protein_id="HDI6044432.1"
                     /translation="MYHLRVPQTEEELERYYQFRWEMLRKPLHQPKGSERDAWDAMAH
                     HQMVVDEQGNLVAVGRLYINADNEASIRFMAVHPDVQDKGLGTLMAMTLESVARQEGV
                     KRVTCSAREDAVEFFAKLGFVNQGEITTPTTTPIRHFLMIKPVATLDDILHRGDWCAQ
                     LQQAWYEHIPLSEKMGVRIQQYTGQKFITTMPETGNQNPHHTLFAGSLFSLATLTGWG
                     LIWLMLRERHLGGTIILADAHIRYSKPISGKPHAVADLGALSGDLDRLARGRKARVQM
                     QVEIFGDETPGAVFEGTYIVLPAKPFGPYEEGGNEEE"
     gene            1541..3355
                     /locus_tag="PN977_004480"
     CDS             1541..3355
                     /locus_tag="PN977_004480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000226314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6044433.1"
                     /translation="MVANINLIKQESYSVVNLEKQLMSNESPKQTLLNDFSHRLKMEG
                     EEQLCDCVNELKLGLSSNNKYDLFKNKETSLWKRKVPESAALAMTGTVYKLFGSRLLN
                     VRNQLDKNLLEMAMQMVYGKDMVTKSGDILIDIQLNKDGSLQSKHYTFDVGEVINSYN
                     IDLFVNASSINQTYLHDKNPGDVISLYGTLDNIPLVVKECTGKNHQINVKAKLPQERS
                     EKVEDMCERMRGGISMFNHTTKTAGNIEHNLQLSFLVDSRPSITNKYSAKDVGEDILA
                     LPVNVYHADFKIKINNAVEKASSIVLCAVQKNLHAARKVLSGIIGNVPKNNKPKTIIF
                     DLRFLSAIKQKVFLGSEKPLFVKHTAMMREACEELPQSVEYVPLACEAHSHRSVALAK
                     TVAAPIMATGVGSALMYATSSGPYYAQSLSGSPDIEMIKNRMAQLFTQLDASVRNKYR
                     PAFDKWNELVDKLNHERNTVPFACLTTILATAINETPEGDTNVAVVMGCKSAKDRTIS
                     IVLGNSMLQTLFEKRLADDGEIENLVDQQGYFNCDSLTAEELMILKDLFDIRVLHVSN
                     KFNVGLQGNINTDVLQDSFFKNVDFIHESSSYTVGMGV"
     gene            4051..4269
                     /locus_tag="PN977_004481"
     CDS             4051..4269
                     /locus_tag="PN977_004481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312842.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HDI6044434.1"
                     /translation="MAMNTVFLHLSEEAIKRLNKLRGWRKVSRSAILREAVEQYLERQ
                     QFPVRKAKGGRQRGETVVVDDQCKEHKE"
     gene            4488..4730
                     /locus_tag="PN977_004482"
     CDS             4488..4730
                     /locus_tag="PN977_004482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312843.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6044435.1"
                     /translation="MNSLAGIDMGRILLNLSNELFKQLDDLEVQRNLPRAELLREAVD
                     QYLVNQSQTARASAFGIWQGSEEDGVEYQRKLREEW"
     gene            complement(5059..5988)
                     /gene="fdhE"
                     /locus_tag="PN977_004483"
     CDS             complement(5059..5988)
                     /gene="fdhE"
                     /locus_tag="PN977_004483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory protein FdhE"
                     /protein_id="HDI6044436.1"
                     /translation="MSIRIIPQDELGSSEKRTADMIPPLLFPRLKNLYNRRAERLREL
                     AENNPLGDYLRFAALIAHAQEVVLYDHPLEMDLTTRIKEASAQGKPPLDIHVLPRDKH
                     WQKLLMALIAELKPEMSGPALAVIENLEKASTQELEDMASALFASDFSSVSSDKAPFI
                     WAALSLYWAQMANLIPGKARAEYGEQRQYCPVCGSMPVSSMVQIGTTQGLRYLHCNLC
                     ETEWHVVRVKCSNCEQSGKLHYWSLDDEQAAIKAESCDDCGTYLKILYQEKDPKIEAV
                     ADDLASLVLDARMEQEGYARSSINPFLFPGEGE"
     gene            complement(5985..6620)
                     /gene="fdoI"
                     /locus_tag="PN977_004484"
     CDS             complement(5985..6620)
                     /gene="fdoI"
                     /locus_tag="PN977_004484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HDI6044437.1"
                     /translation="MKRRDTIVRYTAPERINHWITAFCFILAAVSGLGFLFPSFNWLM
                     QIMGTSQLARILHPFVGVVMFASFIIMFFRYWHHNLINRDDIFWAKNIRKIVVNEEVG
                     DTGRYNFGQKCVFWAAIIFLVLLLVSGVIIWRPYFAPAFSIPVIRFALMLHSFAAVAL
                     IVVIMVHIYAALWVKGTITAMVEGWVTSAWAKKHHPRWYREVRKTTEKKAE"
     gene            complement(6617..7519)
                     /gene="fdoH"
                     /locus_tag="PN977_004485"
     CDS             complement(6617..7519)
                     /gene="fdoH"
                     /locus_tag="PN977_004485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase O subunit beta"
                     /protein_id="HDI6044438.1"
                     /translation="MAYQSQDIIRRSATNGLTPAPQARDFQEEVAKLIDVTTCIGCKA
                     CQVACSEWNDIRDTVGNNIGVYDNPNDLSAKSWTVMRFSEVEQNDKLEWLIRKDGCMH
                     CSDPGCLKACPAEGAIIQYANGIVDFQSEQCIGCGYCIAGCPFDIPRLNPEDNRVYKC
                     TLCVDRVVVGQEPACVKTCPTGAIHFGTKESMKTLASERVAELKTRGYDNAGLYDPAG
                     VGGTHVMYVLHHADKPNLYHGLPENPEISETVKFWKGIWKPLAAVGFAATFAASIFHY
                     VGVGPNRADEEENNLHEEKDEERK"
     gene            complement(7532..>9928)
                     /gene="fdnG"
                     /locus_tag="PN977_004486"
     CDS             complement(7532..>9928)
                     /gene="fdnG"
                     /locus_tag="PN977_004486"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HDI6044439.1"
                     /translation="DIKNANLVVVMGGNAAEAHPVGFRWAMEAKIHNGAKLIVIDPRF
                     TRTAAVADYYAPIRSGTDIAFLSGVLLYLLNNEKFNREYTEAYTNASLIVREDYGFED
                     GLFTGYDAEKRKYDKSSWTYELDENGFAKRDTTLQHPRCVWNLLKQHVSRYTPDVVEN
                     ICGTPKDAFLKVCEYIAETSAHDKTASFLYALGWTQHSIGAQNIRTMAMIQLLLGNMG
                     MAGGGVNALRGHSNIQGLTDLGLLSQSLPGYMTLPSEKQTDLQTYLTANTPKPLLEGQ
                     VNYWGNYPKFFVSMMKAFFGDKATAENNWGFDWLPKWDKGYDVLQYFEMMKEGKVNGY
                     ICQGFNPVASFPNKNKVIGCLSKLKFLVTIDPLNTETSNFWQNHGELNEVDSSKIQTE
                     VFRLPSTCFAEENGSIVNSGRWLQWHWKGADAPGIALTDGEILSGIFLRLRKMYAEQG
                     GANPDQVLNMTWNYAIPHEPSSEEVAMESNGKALADITDPATGAVIVKKGQQLSSFAQ
                     LRDDGTTSCGCWIFAGSWTPEGNQMARRDNADPSGLGNTLGWAWAWPLNRRILYNRAS
                     ADPQGNPWDPKRQLLKWDGTKWTGWDIPDYSAAPPGSGVGPFIMQQEGMGRLFALDKM
                     AEGPFPEHYEPFETPLGTNPLHPNVISNPAARIFKDDAEALGKADKFPYVGTTYRLTE
                     HFHYWTKHALLNAILQPEQFVEIGESLANKLGIAQGDTVKVSSNRGYIKAKAVVTKRI
                     RTLKANGKDIDTIGIPIHWGYEGVAKKGFIANTLTPFVGDANTQTPEFKSFLVNVEKV
                     "
BASE COUNT         2555 a         2372 c         2491 g         2510 t
ORIGIN      
        1 tcgcgcagag gcgttatatg actatttcgt cgaacgctgc cgtcagcaag agatgaacac
       61 gcaaacagga cgcttcgctg cggatatgca ggtatcgctg gtcaatgatg gccccgtgac
      121 attctggttg caggtatgag ccagcttcca gggttgtcac gggaaacaag agagagtatc
      181 gctatgtatc accttcgggt tccacaaaca gaagaagaat tagagcgtta ctatcagttt
      241 cgctgggaaa tgttgcgtaa gcccctgcat caaccgaaag gttcggaacg cgacgcgtgg
      301 gatgcgatgg cgcatcacca gatggtcgtc gacgagcagg gtaatctggt ggcggtaggc
      361 cgactgtata ttaatgccga caatgaagcg tccattcgct ttatggccgt tcatcccgac
      421 gtgcaggaca aagggttagg aacgctgatg gcgatgaccc ttgagtcggt ggcgcgtcag
      481 gaaggcgtta agcgcgtgac ctgtagtgcc cgtgaagacg cggtggagtt ttttgccaaa
      541 ctggggtttg tcaatcaagg ggagatcact accccaacca ccacgccgat tcgccatttt
      601 ttgatgatta aacccgttgc cactctggat gatattttgc atcgcggcga ctggtgcgcg
      661 cagctgcaac aggcgtggta cgaacatatc ccgctaagcg aaaaaatggg cgtgcgcatt
      721 cagcaatata ccgggcaaaa atttatcacc accatgccgg aaaccggtaa tcagaatccg
      781 caccatacgc tgtttgccgg gagtttattc tcactggcaa cgctcaccgg ttggggactt
      841 atctggctga tgctgcgtga acgccatctg ggcggaacga ttattctggc ggatgcgcat
      901 attcgctaca gcaagccgat tagcggtaaa cctcatgcgg tagccgacct cggtgcctta
      961 agcggcgatc tcgaccgtct ggcgcgcggg cgcaaagcgc gggtacaaat gcaggtcgaa
     1021 atctttggcg acgagacgcc gggtgcagtg tttgaaggca cgtatatcgt tctgcccgcg
     1081 aagccatttg gcccctatga agagggcggg aacgaagaag agtagctgat ggagaggggc
     1141 ggtgctgcct ctctcattct ttcaggtcat ttacatcagt ctctgactat tccggttata
     1201 ttgaataaga actctataaa tttttattct tcgttggcac catacgtgtc ttgctacatc
     1261 actgttcagg agcatttaaa tattatttgt agagctgagt ggcaattatc gctgataatg
     1321 cttaattcat catcttagtt caggtttgag cgatttttga tagtcaaaac cccctctggt
     1381 gcaatacttt tctcataaat atttttaaaa tctatagcta acaaacatta tgcaaagata
     1441 atggactggc gtcattgtca gtatcattca tttactgctt ctggatgttg taaataatta
     1501 tgtggcatgt ttgctcttaa accgtgggaa ggaggctgta atggttgcca atattaatct
     1561 cattaaacaa gaaagttatt ctgttgttaa tttagaaaaa caactaatga gtaatgaatc
     1621 accgaagcaa acgctattaa atgatttttc acatcgcctt aaaatggaag gtgaggagca
     1681 actgtgcgat tgtgttaacg aacttaaact cgggttgtcg agtaataata aatatgacct
     1741 gtttaaaaat aaagaaacct cactgtggaa acgcaaagta ccagaaagtg cagcgctggc
     1801 aatgacggga acagtatata aattatttgg tagtcgctta ctcaacgtcc gcaatcagtt
     1861 agataaaaat ctcctcgaaa tggctatgca aatggtttat ggcaaagata tggtgacaaa
     1921 gtccggggat atattgattg atatacaatt aaacaaggat ggcagcctcc agagtaagca
     1981 ctacacattt gatgtaggtg aagttataaa cagttataat attgacctct ttgtgaatgc
     2041 ttcgagtatt aatcaaactt acttacatga taaaaatcct ggtgacgtta ttagcctgta
     2101 tggcacttta gataacattc ctcttgtagt taaggaatgc acgggtaaaa atcatcaaat
     2161 taacgttaaa gccaaattgc ctcaagaacg aagtgaaaaa gttgaggata tgtgtgaaag
     2221 aatgagaggt ggcatctcta tgtttaatca cactacaaaa acagctggta atatagagca
     2281 taatttgcag ctaagttttc tcgtggattc acgaccgtcg atcacaaata aatactcagc
     2341 caaagatgta ggggaagata tacttgcatt gcccgtcaac gtatatcatg cagattttaa
     2401 aattaaaatc aataatgcag tagagaaggc ttcgtcaatt gtgttatgtg ctgtgcaaaa
     2461 aaatctccat gcagcaagaa aggttcttag cgggataata ggaaatgtgc caaaaaataa
     2521 taaaccaaaa acaatcattt ttgatttacg ttttcttagt gcaattaagc agaaagtttt
     2581 tttgggcagt gaaaaaccgt tgtttgtaaa gcataccgca atgatgaggg aggcatgtga
     2641 ggaattaccg cagtcagtgg aatatgttcc tttagcctgt gaagcgcata gccatcgcag
     2701 cgtcgccctt gccaaaaccg tggctgcgcc catcatggcg acaggtgtgg gctcggcttt
     2761 aatgtatgca acatcatcag gaccgtatta tgcacaaagt ctttctggtt cgccggatat
     2821 cgagatgata aaaaatcgca tggctcaatt gtttacccag ttggatgctt ctgtgcgcaa
     2881 caaatatcgc ccggcttttg ataaatggaa tgagctggta gataaattaa atcacgaaag
     2941 aaatacagtt ccgtttgcgt gtttgaccac cattttagcg acggcgataa atgaaactcc
     3001 tgagggggat acgaatgttg ccgttgtgat ggggtgtaaa tctgcgaaag atcgcacgat
     3061 atccattgtc cttggcaact cgatgttgca aacactcttt gaaaaacgcc tcgctgatga
     3121 tggagaaata gaaaatcttg ttgatcaaca gggatatttt aactgcgatt cattaacagc
     3181 cgaagaatta atgatattaa aagacctttt tgatattcga gttcttcatg tctctaacaa
     3241 attcaatgtt gggttgcaag gtaatatcaa cactgatgtt ttacaagatt cgttttttaa
     3301 aaatgtcgat ttcattcatg aatctagctc gtataccgta gggatgggag tctgacgggt
     3361 aaaactcatt aggattatca gaattcggac aatgcattgc aaaaatgaca ttcaaagaga
     3421 gatgtgtgca ggcgcttagc cgcgaggggc atggcgatgc catacagaaa gcaccagcat
     3481 tcagaactcc tgtgaaagcg gcatagtggc cgcctgtgca ggagttttgc tatgacccgt
     3541 atcgtgctga ccgttgccac cctatgatgc gattaaaaaa actcatcgtc gcgccgatga
     3601 ttgacgtcaa tgctatcgga tgataccggg ggtgttccgg aaggaattcc ttgtttccca
     3661 aactgttacc aacaaaatta taacgacgag caagtaaaga ccggttggtg gcagcattga
     3721 acggtctgtg atggatgtta aatttcacct taaatcctgg tgctgttagg atgctgacgt
     3781 ttttcgggta caccattcag attatgcaaa tgaagaccca atcaaaaaat aaatatccct
     3841 gaatataata tatttatagc acccatatct catcctgggc gataactaaa ccatttttga
     3901 ttaacaaatg aaacaactgc aacgttctta ttatttctgg aaaacgtctc gcaaaaacaa
     3961 aacagcatgc aaaaatccgc tttttttaaa tgatttttcg gcgtaatttt cgcacataca
     4021 ttaatgagta atatatgtaa ggtattggta atggcgatga atacggtttt tcttcattta
     4081 tcagaagagg caattaaacg gctgaacaag cttaggggct ggcgtaaggt ttcacgatct
     4141 gcaattttac gcgaagcggt agagcaatat ctggaacgtc agcaattccc ggtgcgtaaa
     4201 gcaaagggag gcaggcaaag gggtgagaca gtagttgttg atgatcagtg taaggagcat
     4261 aaggaatgac aaaaaatata ttattcttga atataaaaac cagatgccct tattctgata
     4321 ttaatacaag gttgttttac ttgaacttat aataactgca actgttacat cgtatctgga
     4381 aaacgcctcg caaaaataaa aaatgatgcg taaatgcgct ttctttaaag attcggcaag
     4441 ggtacttttc tctccataga ttcaaaaatg atattggcga gatatttatg aactctctgg
     4501 caggtataga tatgggcaga attttactca atttatcgaa tgagttgttt aagcaacttg
     4561 atgatctcga ggtgcagcgt aatcttcctc gtgcggagct attacgggaa gcggtagatc
     4621 aatacctggt aaatcaatcg caaacagcaa gagccagtgc ttttggcatc tggcaaggga
     4681 gtgaggaaga tggtgtcgaa taccagcgta agctgcgcga ggaatggtaa tggcaaaaat
     4741 gataccaatc agattgctga caacgagcgc gttgttcatg ctggatgcgg cgtgaacgcc
     4801 ttatccggcc tacaaagtca tgcaaattca atatattgaa gtttattggt aggcctgata
     4861 agcgcagcgc atcaggcaat catacgcttg tcatcagtga catacccata accttatcga
     4921 tgtgttggag ggaagaacga ggcaaacaga caattgcggc atgatcttga atgggtaacg
     4981 cggaatacga gagatagtgt cgtcacaccc tatgagctgt aatccgggcg acctgcgcca
     5041 cccggaaaac gtcagagatt actccccttc acccggaaac aagaacgggt taatagaact
     5101 acgggcgtag ccttcttgct ccattcgcgc gtccagtacc agagaggcga ggtcatctgc
     5161 tacggcttcg atttttggat ctttttcctg atagagaatt ttcaggtaag tgccgcagtc
     5221 atcgcagctt tcggctttaa tcgcggcctg ttcgtcatcc agcgaccagt aatgcagttt
     5281 gccgctctgc tcgcagttgc tgcattttac gcgcactacg tgccattcgg tttcacacag
     5341 gttgcagtgc aggtaacgca gcccctgagt ggtgccaatt tgcaccatgc tggacaccgg
     5401 catagagcca cataccgggc aatattgacg ttgttcgccg tattcagcgc gggctttgcc
     5461 ggggatcaga ttagccatct gcgcccagta gagcgacagc gcagcccaga taaacggcgc
     5521 tttatcgctg ctgacggacg agaaatcaga ggcaaacagt gcgctggcca tatcttccag
     5581 ctcctgagtc gatgccttct ccagattctc aatcactgcc agcgccgggc cgctcatttc
     5641 aggtttcagc tcagcaatca gcgccatcag cagcttttgc cagtgcttat cacgcggcag
     5701 aacgtgaata tccagcgggg gcttgccttg tgcgctggct tctttaatgc gcgtggtcag
     5761 atccatctcc agcggatggt cgtacagcac cacttcctgg gcgtgggcga taagcgcagc
     5821 aaagcgcagg taatcaccca gcggattatt ttctgccagc tcgcgcagac gctcggcgcg
     5881 gcggttgtat aaattcttga gccgagggaa caataacggc ggaatcatat ccgccgtacg
     5941 tttctcgctc gaacccagct catcttgcgg gattatgcga atactcattc agctttcttt
     6001 tccgttgtct tgcggacctc acggtaccag cgcgggtgat gtttcttcgc ccaggcgctg
     6061 gtaacccatc cttccaccat ggcggtaatc gtgcctttca cccaaagggc ggcgtagata
     6121 tgcaccatga taaccacaat taacgccact gcggcaaatg aatgcagcat taacgcgaat
     6181 cggatcaccg ggattgagaa agcaggcgca aaataaggac gccagataat cacgccgctc
     6241 accagcagca gaaccaggaa aataatcgcc gcccagaaaa cgcatttctg accgaagtta
     6301 taacgcccgg tgtcgcctac ttcctcgttg acgacgatct tacgaatatt cttcgcccaa
     6361 aagatatcat cccgattgat taggttgtga tgccagtaac ggaaaaacat gatgatgaac
     6421 gaggcaaaca taaccacgcc gacaaacggg tgcagaatgc gcgccagctg cgatgtgccc
     6481 atgatttgca tcaaccagtt gaaggacggg aacaaaaagc ccagcccgct caccgccgcc
     6541 aggatgaagc agaaggcggt gatccagtgg ttgatacgtt ccggcgcggt gtagcgcacg
     6601 atggtgtcac gtcgtttcat ttgcgctcct cgtctttctc ttcgtgcaga ttattctctt
     6661 cctcatccgc acggttcgga ccgacaccca cgtagtggaa gatactggct gcgaaggtag
     6721 ccgcaaagcc aacagctgcg agcggtttcc agatgccttt ccagaatttc acggtttcgc
     6781 tgatttccgg gttctccggc aagccatgat acagatttgg cttgtcagca tggtgcagca
     6841 cgtacataac gtgtgtacca ccgacgcctg ccggatcgta cagacccgca ttgtcgtaac
     6901 cacgggtttt cagctccgcc acgcgctcgc tcgccagcgt tttcatcgac tctttcgtac
     6961 cgaagtgaat cgcgcccgtt gggcaggtct tcacgcaggc cggttcttgc ccaaccacca
     7021 cgcggtcaac gcacaacgta catttgtaga cgcggttatc ttccgggttg aggcgcggaa
     7081 tgtcgaacgg acagcccgca atgcaataac cgcagccgat gcactgctcg gactggaagt
     7141 cgacgatacc gttggcatac tgaataattg ccccttccgc cgggcacgct ttcaggcagc
     7201 ctggatcgga acagtgcata cagccgtctt tgcggatcag ccattccagt ttgtcgttct
     7261 gctccacttc cgagaatcgc atcaccgtcc acgatttggc acttaaatca ttggggttgt
     7321 cgtacacccc aatgttattg ccgacggtat cgcgaatgtc gttccactct gaacacgcca
     7381 cctgacaggc tttacagccg atacaggtgg tgacgtcgat gagtttcgcc acttcttcct
     7441 ggaagtcccg cgcctgaggc gcgggggtca gaccgttagt cgcggaacga cgaatgatat
     7501 cttgcgattg ataagccata tgtcgtctcc gttacacctt ttccacattc acaaggaagg
     7561 acttaaactc cggcgtctgc gtgttcgcat caccgacgaa tggcgtcaac gtattggcaa
     7621 taaagccttt tttcgcaaca ccttcatagc cccagtgaat cggaataccg atggtatcga
     7681 tatctttgcc gtttgctttc agcgtgcgaa tacgtttggt cactaccgct ttggctttga
     7741 tatagccacg gttggaggag actttcacag tatcgccctg ggcaatgcca agtttattcg
     7801 ccagcgactc cccgatttcc acaaactgct ctggttgcaa aatcgcgttc aacagcgcgt
     7861 gtttggtcca gtagtggaag tgctcggtca gacgataggt ggttccgaca tacgggaact
     7921 tatcggcttt acccaatgct tcggcgtcgt ctttaaagat tcgcgcagcc gggttcgaga
     7981 taacgtttgg atgcagcggg ttagttccca gcggcgtttc aaacggctcg tagtgttccg
     8041 ggaacggacc ttccgccatc ttatcgaggg caaacagacg ccccatgcct tcctgctgca
     8101 tgataaacgg cccgacgcca ctgcccggag gcgctgcgct gtaatccgga atatcccagc
     8161 cggtccactt agtgccgtcc catttcagca actgacgctt cggatcccat gggttaccct
     8221 gcggatctgc ggaggcgcgg ttatacagaa tgcggcggtt aagcggccat gcccatgccc
     8281 agcccagcgt gttaccgagg ccagacggat cggcgttatc acgacgcgcc atctggttgc
     8341 cttccggcgt ccagctaccg gcgaaaatcc agcagccaca ggaggttgta ccgtcatcgc
     8401 gcagttgggc gaacgagcta agttgttggc ctttcttgac gataaccgca ccggttgccg
     8461 gatcggtaat atcggccagc gccttaccgt tactctccat cgccacttct tctgaagatg
     8521 gctcatgcgg aatggcgtag ttccaggtca tgttcagcac ctggtccggg ttcgcgccac
     8581 cctgttcggc atacatcttg cgcaagcgca ggaagatacc ggagaggatc tcgccgtcag
     8641 tcagcgcaat ccccggggcg tccgcacctt tccagtgcca ctgcaaccag cggccagagt
     8701 tgacgattga accgttctct tccgcgaagc aggtcgatgg cagacggaac acttcggtct
     8761 ggatcttcga cgagtcaact tcgttcagtt caccgtggtt ctgccagaag ttagaggttt
     8821 cagtgttcag cgggtcgatg gtcacgagga acttcagttt cgacagacag ccgatcactt
     8881 tgtttttgtt cgggaatgag gcaacagggt taaagccctg gcagatatag ccattgacct
     8941 tgccctcttt catcatctcg aagtattgca ggacgtcgta gcctttatcc cacttcggca
     9001 accagtcaaa gccccagtta ttttccgccg tcgctttatc accaaagaag gccttcatca
     9061 tggagacgaa gaatttcggg taattgcccc agtagttaac ctggccttcc agcagtggtt
     9121 tcggcgtgtt ggcggtaagg taagtttgca gatcggtctg cttctcgctt ggcaacgtca
     9181 tgtaacctgg cagactctgc gacagcagcc ccaggtcggt cagcccctga atattggagt
     9241 gaccgcgcag ggcgttaacg ccgccgcctg ccatccccat gttgccgagc agcagctgga
     9301 tcatcgccat cgtacgaatg ttttgcgcac caatggagtg ttgcgtccag ccgagcgcgt
     9361 aaaggaacga ggcagtttta tcgtgagcac tggtctctgc gatgtattcg cagactttca
     9421 ggaacgcgtc tttcggcgta ccacagatgt tttcaaccac atctggcgta taacgggaaa
     9481 cgtgctgttt cagcaagttc cacacgcagc gcggatgttg cagcgtggta tcgcgtttgg
     9541 cgaagccgtt ttcgtccagc tcgtaggtcc agctggattt atcgtacttg cgtttttccg
     9601 cgtcgtagcc ggtgaacagg ccatcttcaa agccgtaatc ctcacgcacg atcaggctgg
     9661 cgttggtata ggcttcggtg tattcgcggt tgaatttttc attgttcagc aggtacagca
     9721 atacgcctga caggaaagca atgtcagtcc cggaacgaat aggggcatag tagtcagcca
     9781 ccgccgccgt acgtgtaaag cgaggatcga tcacaatcag cttcgcgccg ttgtgaattt
     9841 tggcttccat cgcccagcgg aacccgaccg ggtgagcttc agcggcgtta ccgcccatca
     9901 ccacgacgag gttggcattc ttgatgtc
//
LOCUS       DAMZFT010000173         9712 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.173, whole genome shotgun sequence.
ACCESSION   DAMZFT010000173 DAMZFT010000000
VERSION     DAMZFT010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9712)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9712)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9712
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.173"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..1164
                     /locus_tag="PN977_004487"
     CDS             <1..1164
                     /locus_tag="PN977_004487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001131672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDI6044440.1"
                     /translation="AKPADKDYKLSDGQGLYLLVKKSGARYWNLKYRFAGKEKKLVIG
                     VYPDISLAQARQKRDEARKLLAEGKDPSYEKQARKLAIRESMACTFRTIAEELLTHMA
                     RRWTTGYQADQRARLENHVFPALGNRPVTGIRPMEILNVLRTIEEQGKLEALRKTRQL
                     CAQVFDYAIATGRATVNPASTLSGVLLPPEKQQRRTLSEEQIGAFMYQITASGNLMSL
                     ATRMLVLTAVRTGELRLATWDEINFTEALWVIPKERMKMRRPHVVPLSRQVLALLEEI
                     KTRTESRNSAFIIPGAFRKDLPRNRKCINEFLEQSGWHALTTAHGFRHTFSTILHDHD
                     FSSAWIELQLAHVDKNVMRGTYNHAQYLDGRREMMQWYADYIDSLMEKNVKTK"
     gene            1531..2586
                     /locus_tag="PN977_004488"
     CDS             1531..2586
                     /locus_tag="PN977_004488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000999864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HDI6044441.1"
                     /translation="MNRLRFEVKNNQGCFIFPETWYESLLEKFNELLDSFDEDEISDR
                     SYINALKRITQQEPDFIDAYAYMAYAFLEQETPRKALNIALKGLAAGNRLIPEGFTGE
                     ISWLHPENRPFLNALYAAILANVHLQHHQNAVMLIDKILAYNPSDNPGARWLLGPELL
                     RSGDHERALCVLRQYADEFSPYWYELGLLHFINGEPVKAATAFRRGFAENTYIAEILC
                     GNLYPFPLALWHDYSSEPDTARQYYETFCPLWGKYPEALLFVNWLYNQSGVLSERAEF
                     MKCAEMLLRADDQEMNESIFQQRELLRERIDDRLSEEIVQKLRTLNGEEIWPWMLPFS
                     GELIPADICFQNSLKYQ"
     gene            complement(2688..3020)
                     /locus_tag="PN977_004489"
     CDS             complement(2688..3020)
                     /locus_tag="PN977_004489"
                     /inference="COORDINATES: protein motif:HMM:NF033564.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
                     /protein_id="HDI6044442.1"
                     /translation="MTIAVCGVLVGADNFQEIELWAGNALSGSASFLPLKNGIPSHDT
                     FARLFGLIVPEQFEAAFRRWGSEVLPVLSSQVVAIDGKTSRRSGSRCQSPLHRGNQSA
                     MVYCSDLG"
     gene            3196..3312
                     /locus_tag="PN977_004490"
                     /pseudo
     CDS             3196..3312
                     /locus_tag="PN977_004490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013183589.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(3631..4287)
                     /locus_tag="PN977_004491"
     CDS             complement(3631..4287)
                     /locus_tag="PN977_004491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010308382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EcsC family protein"
                     /protein_id="HDI6044443.1"
                     /translation="MTDNENILTPSKIIKFLDYAYEKSIDGIGIPGIDTAHSLAESYL
                     KTGSSLKEQINSLIRWQNTKSATSGFLTGLGGIITLPVTIPTNISSVLFIQVRMVASI
                     AIMCGQDIRDDKVRTIVYTCLVGNAAKDILKEAGIQIGQKLTTNAIRCISKDIIVKIN
                     KAVGFRLLTKTGATGVINMSKFVPVVGGIVGGSLDAITTNIVGNYARDTFLSLIDNDL
                     "
     gene            complement(4661..5014)
                     /locus_tag="PN977_004492"
     CDS             complement(4661..5014)
                     /locus_tag="PN977_004492"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6044444.1"
                     /translation="MGFWDAIMGFGKLASNAAAETMKKANFNVWDKIKSSPVERINDF
                     YNQNNTSEHNRPACRGLAIAALCFRGDWSGERLWREDQEAANWLKNFRIKISLDTCDS
                     ADELRKIIDRLTELN"
     gene            complement(5227..5976)
                     /locus_tag="PN977_004493"
     CDS             complement(5227..5976)
                     /locus_tag="PN977_004493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001412695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HDI6044445.1"
                     /translation="MKKLLALTAVAALTGTFSTLTLADADKTGFYITGKAGASVITQD
                     NQRFSQDFGDDVYKYKGGDKSDTVFGAGLAVGYDFYQHYNVPVRTEVEFYGRGSANSR
                     YPLDTWESPSGDNGWKEDTQNRLSVNTLMVNTYYDFRNSSAFTPWVSAGLGYARVHHK
                     ATYTDTSWNKSGEVSDASSLHYSGYDNNFAWSLGVGVKYEVTPDFSLDLAYRYLDAGD
                     STLTYEDGQDTYKSSVDVRSNEFTLGATYSF"
     gene            6292..6645
                     /locus_tag="PN977_004494"
     CDS             6292..6645
                     /locus_tag="PN977_004494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001350516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6044446.1"
                     /translation="MNKALSSILLIFGICASSLAAEKNRTVSFPRCEVQEASAVAASV
                     KRDYLQKQVFQWKDDQEILGQQRPVVWVNLREPVNKGDNVITVHLTARGKKADIQYRV
                     RVDCKNGSATWQRQG"
     gene            complement(6692..7264)
                     /gene="yraP"
                     /locus_tag="PN977_004495"
     CDS             complement(6692..7264)
                     /gene="yraP"
                     /locus_tag="PN977_004495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001238641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divisome-associated lipoprotein YraP"
                     /protein_id="HDI6044447.1"
                     /translation="MQRWFLPFLSTLLLLQGCVAAALVGTAAVGTKVSVDPRSAGTQI
                     DDEILEARVSRALSKDSQLRGDARIIVTAYQGTVLLTGQAPDRSAGEKARQIADGVEG
                     TVQVWNEIRTGKPLRADKTATDAWLTTKIRAQLLKSSRVKLSDVKVLTENGEVFLMGR
                     VTEKEGQAAADIASRVAGVKHVITAFSYIR"
     gene            7588..7838
                     /locus_tag="PN977_004496"
                     /pseudo
     CDS             7588..7838
                     /locus_tag="PN977_004496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001354604.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(8006..8440)
                     /locus_tag="PN977_004497"
     CDS             complement(8006..8440)
                     /locus_tag="PN977_004497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010280495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HDI6044448.1"
                     /translation="MPARINMFFTRLTDYPDTGLLLLRLTFGILMLFHGVAKIEHGVN
                     WIISMLQDAGLPGFIAYGVYIGEIVAPVLIILGIFTRPAALIYALTLIVATLLVGTGK
                     FFTLTEVGAWGLENEMLYFMGGIAIMLLGPGQYSVLTRTSLR"
     gene            9026..9199
                     /locus_tag="PN977_004498"
                     /pseudo
     CDS             9026..9199
                     /locus_tag="PN977_004498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001724266.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            9418..>9712
                     /locus_tag="PN977_004499"
     CDS             9418..>9712
                     /locus_tag="PN977_004499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000949004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HDI6044449.1"
                     /translation="MHSIKKVTMLLGCLALTCSIAFQASATEKFKVITTFTIIADMAK
                     NVAGDAAEVSSITRPGAEIHEYQPTPGDIKRAQGAQLILANGMNLELWFQRFYQ"
BASE COUNT         2804 a         2273 c         2007 g         2628 t
ORIGIN      
        1 gctaaacctg ctgacaagga ttacaaactt tctgacggtc agggccttta tcttcttgtc
       61 aaaaaatccg gtgcccgtta ctggaatctg aagtaccggt ttgccggtaa ggaaaagaaa
      121 ctggtgattg gtgtctatcc tgacatatca ctggctcagg ctcgtcagaa gcgggatgaa
      181 gcacgtaaac ttctggctga aggtaaagat ccgtcgtatg aaaaacaggc gcgaaaactt
      241 gccatcaggg aaagtatggc ctgcacattt cgcaccattg cagaggagct gcttactcat
      301 atggcacgtc gatggacaac aggttaccag gcagaccagc gtgcaagact tgaaaatcat
      361 gttttccctg ctcttggaaa ccgtcctgtg accgggatcc gccccatgga aatactgaac
      421 gtcctgagga ctatagaaga acagggaaaa cttgaagcac tgcgtaaaac acgccagctc
      481 tgtgcgcagg tttttgatta tgccatagca acagggcgtg caaccgttaa tccggcatcg
      541 acactcagcg gggtactgct tcctcctgaa aaacaacagc gcaggacgct ttctgaagaa
      601 cagataggag ctttcatgta tcagataacg gcttccggaa atctgatgtc gctggcaacg
      661 cggatgctgg ttctgaccgc tgttcgcacc ggagaactgc gactggcaac ctgggacgaa
      721 attaatttca cggaggccct gtgggttatt ccgaaagaaa gaatgaaaat gcgacgaccg
      781 cacgttgtgc cgctttcacg gcaggtactg gcgttactgg aggagataaa aacacgcact
      841 gaatcgcgta attccgcctt tattattcct ggtgcattca gaaaagatct cccgcgaaac
      901 cgcaagtgta tcaatgaatt ccttgagcag tcaggctggc atgccctgac aacagcccac
      961 ggatttcgtc ataccttcag caccattctc catgaccatg atttcagctc agcctggatt
     1021 gaactccagc ttgctcatgt tgataaaaac gtcatgcgtg gcacttataa ccatgcgcaa
     1081 tatctggatg gacgtcgtga aatgatgcag tggtacgctg attacattga ttcacttatg
     1141 gagaaaaacg taaaaacgaa ataagccatc acccccacca accacagcgg ttggtgggga
     1201 gttaactacc atttgtattt tttgatttct gccttcaggc gacatactca atatcatctg
     1261 atgagcgaag ataccccacc caaaatgatg gtagcttcac atcgtcattt tcatatagca
     1321 gcacactgct gttctgacct cgtagtcaca tttctacagc atcattacca gtcaggctga
     1381 ttgctcctca ttgaaagaat gaatccatga ctaacacaaa aatagctctg gccggctgtt
     1441 accggtgtga gaagaaactt gatacaaact gccgtgggct ttattcttgg agaactataa
     1501 gtttttccag atttacaaca gattcagcat atgaacagat tacgctttga agtgaagaat
     1561 aatcagggct gcttcatttt cccggaaacc tggtatgaat cactactgga gaagtttaac
     1621 gaactgcttg actcctttga cgaggatgag atcagcgaca ggtcttatat caatgccctg
     1681 aagcgtatca cacaacagga accggatttt attgatgcgt atgcatacat ggcatatgcc
     1741 tttctggagc aggagacacc gcgaaaagcg ctgaatattg cactcaaagg actggcggca
     1801 ggtaaccgac tgatacctga aggtttcacc ggtgaaatca gctggttaca tccggaaaac
     1861 aggccctttc tgaatgccct ttacgccgcc atcctcgcga atgtgcacct gcagcatcat
     1921 cagaatgctg tcatgctcat tgataaaatt ctggcttaca atccttcaga taatccggga
     1981 gcacgctggt tactgggacc ggaactgttg cgttctggtg accatgaacg ggctctctgt
     2041 gttctccggc agtatgcaga tgaattttcg ccttactggt atgagcttgg tcttctgcat
     2101 tttattaatg gagagccggt gaaggcagcg acagctttcc ggcgcggctt tgcagaaaat
     2161 acttatatcg cggagattct atgcggaaat ctgtacccct tcccgcttgc cctttggcat
     2221 gattattcat ctgagccgga tacagccaga cagtattatg aaacattctg cccgttatgg
     2281 ggaaaatatc ccgaggcatt gttatttgtt aactggctct acaaccagtc tggcgtactg
     2341 agcgaacggg ctgaattcat gaagtgtgct gaaatgctgt taagggcaga tgatcaggaa
     2401 atgaatgaaa gcatttttca gcagcgggag ctcttacggg aaagaattga tgatcgtctt
     2461 tctgaggaaa tcgttcagaa attacggaca ttgaacgggg aggagatctg gccctggatg
     2521 ttaccgtttt ccggagaact gatccccgca gacatttgct ttcagaacag tctgaaatat
     2581 cagtaatatc tgcaccagga tgccattccg gggtcagtaa ggatcttccc cctgacctgg
     2641 acaaatgtaa tgaaactcag ctaagcccca gaagagcagg catatagtca accgaggtcg
     2701 ctgcaataaa ccatcgcgct ttgattcccc cgatgaagag gtgactggca gcgacttcct
     2761 gagcgacgac ttgtctttcc atcaatagcg actacctgag aagataaaac aggaaggact
     2821 tctgaacccc atcgccggaa cgcagcttca aactgctcag ggacaatcag cccaaaaaga
     2881 cgggcgaatg tatcatggga aggtatgcca ttttttagag gtaaaaaact ggcggagcca
     2941 ctcaatgcgt tgcctgccca gagctcaatt tcctgaaaat tatccgcacc gaccagcaca
     3001 ccacaaacgg caatggtcaa tgtatcaacc agagtgtgcc tgacttgtcc agggctgaca
     3061 gggatcatgt attgagatga atatatcggt aaccggcggt atatgagaca taatatatcc
     3121 ctttgataaa aaaggttatg ttgacatatt tatcctgcat gtcacgtatc attatttttc
     3181 atgcgattgc cctgcggtta ccaccgctca cagttagtaa aggactgggc ggaagtggtc
     3241 aatataaggt tgcattacct tcctccttat agtccgaatc tgaatccgat tgaacggatg
     3301 tggaaaaatg ctgcttaata gtattgatgt tcatctttat gcctctaaaa gaaagcataa
     3361 gatcacaaca ggctcagcag gtcaatcact ctatttttgt tcaaaaaacg ttcatgatct
     3421 tgccctaccc aattttgtgg gtaaggatat gcgatgaaat tctccttgca gtttaataca
     3481 acctttccat attaagcggc agtattacct gccagtcagg ataattttac acctaatgtt
     3541 agttaaaatt tgtgcaactt gaaacaataa tactacagta tccactatcg tcagcacacc
     3601 tcaaatacta ttttttatga aacgtttatt ttataaatca ttatcaatca aactcaggaa
     3661 tgtatctctg gcataattac caacgatgtt ggttgttatc gcatcaagac taccaccaac
     3721 gattcctcct acaacaggaa caaactttga catattaata acaccagtag cacctgtttt
     3781 tgtaagcagt ctgaaaccga ctgccttatt tatctttaca ataatatctt tactaataca
     3841 tctaattgca tttgttgtca acttttgtcc tatctgaatt cctgcttcct tcaaaatatc
     3901 tttcgcagca ttacctacaa gacaggtata aacaatagtt ctgactttat catcacgaat
     3961 atcctggcca cacatgatcg caatagatgc aaccatcctg acctgaatga aaaggacact
     4021 tgaaatattg gtcggtatag ttactggtaa tgtaataatt cctccaagac cagtaagaaa
     4081 acctgatgtg gctgacttgg tattttgcca tcgaataagg ctatttatct gttcttttaa
     4141 actactaccc gttttcagat aactttccgc aagactatgt gccgtatcaa ttcctgggat
     4201 accaatacca tcgatggatt tttcataagc gtaatccaga aattttatga tttttgatgg
     4261 cgtgagaata ttttcattat cagtcataca acaaaacacc taaaaatttc acgatcactt
     4321 tctaaaaact atctccgaca gatatcggaa caccagtatt atcaccaatt aaataatatt
     4381 attttttgag aaacaattgt attgctctgg caaaaagatt ttctgtaatt gtagccttac
     4441 gtttataaat aactttctct ccacacacag gacattcata aaatcctgct gtacaattca
     4501 tatgaataat agttttactc cattgacaat ttgtacagat aaatgtaatg ggtggacagg
     4561 gaaaaatcat tggctcacac tctcgatata taaataacac ttacagggac atgtaaatat
     4621 tttcgattta tccctataaa aataacttca cctccagtaa tcaattcagt tctgtaagac
     4681 gatcgattat tttacgtagt tcatctgcag aatcacatgt atccagtgaa atttttattc
     4741 taaaattctt taaccaatta gcagcctcct gatcttcacg ccataaacgc tccccgctcc
     4801 aatcccccct aaaacataga gcagcaatag ccaatcctct gcatgctggc cgattatgct
     4861 cagatgtatt attttggtta taaaaatcgt taattctttc taccggagat gattttattt
     4921 tatcccacac attgaaatta gcttttttca tcgtttcagc agcagcatta gaagcaagct
     4981 taccgaatcc cattatagca tcccaaaatc ccatatatca ccttatgcta tataatatct
     5041 aaatgagtat ctaatctttt agaaccacta tttggacaat ggttttacgc cacttacatc
     5101 tggtacaaat gaatgtaacc gggggctgag gaagtgccat gaaggtacgt gtctgttgta
     5161 ttaaactatc aaatcagacc acacgctgcg attttgaatt taacaacgtg tagtcagata
     5221 ttacattcag aagctgtatg tagcacctaa cgtaaattcg ttacttctga catcaacaga
     5281 ggatttatat gtatcctgcc cgtcttcata cgtcagggtt gaatcacctg catcaagata
     5341 gcgataagca aggtcaagac tgaaatccgg ggtgacctca tatttcacgc caacaccaag
     5401 gctccatgcg aaattgttat catatcccga gtaatgtaat gaagaagcat cacttacttc
     5461 acctgattta ttccaggagg tatcggtgta tgttgcctta tgatgtaccc gcgcataacc
     5521 cagtccggct gatacccacg gagtgaaagc actgctgttt ctgaaatcat aatacgtgtt
     5581 caccatcagg gtattcacgc tgagcctgtt ttgtgtgtct tccttccaac cattatctcc
     5641 agacggggac tcccatgtat ccagtggata gcgggaattc gcagagccgc gaccatagaa
     5701 ttcaacttcc gtgcgtacag ggacattgta gtgctgataa aagtcgtatc ccaccgcaag
     5761 gccagcacca aaaaccgtat cgctcttgtc accgccttta tacttataaa catcatcccc
     5821 aaagtcctga ctaaagcgct gattatcctg agtgataaca gatgcaccag ccttacccgt
     5881 gatatagaaa ccggttttat ccgcatctgc cagcgtcagc gttgaaaacg taccggtaag
     5941 cgcagctacc gcagtgagtg ccagtaactt tttcacaatc cacctctcct gatgtttcct
     6001 tcagacatat gaatttgtcc aattttaaga cggatttctc attggtaaaa tgaattgtac
     6061 agatcgtatc gatcgacaaa atcgatcgtt aagcagaaaa aagatttatc gtcagcgaag
     6121 atttgtttat ggaaatgact gaaaaaacgg gttaaaaaac agactgttct gtccatgtga
     6181 aacgggaagt gacgggataa gacgttatgt gaggattgtt acgggaaatg ctgttttacg
     6241 ttgttaaaat gtaactccgc aatgaaacta tcccctgtgg aggtcctgta aatgaacaaa
     6301 gcactctctt ccattctgct catcttcggt atctgtgcat catcactggc cgctgaaaaa
     6361 aacagaaccg tcagttttcc acgatgtgaa gtacaggaag catcagctgt tgcagccagc
     6421 gtcaaacggg actatctcca gaaacaggta tttcagtgga aagatgatca ggaaattctc
     6481 ggtcagcagc gtcccgttgt ctgggtgaat cttcgtgagc cggtaaataa aggtgataat
     6541 gtcatcacag tgcatctcac tgcccgtgga aagaaagcag atattcagta tcgtgtgcgc
     6601 gttgactgta agaacggaag tgctacctgg caacgccagg gatgaacggg tgaagttgaa
     6661 tacagagccc gaacgaacgg gctctccata ctcatcgtat ataactgaat gcggtgatga
     6721 cgtgtttcac ccccgccacg cgactggcga tatcagcagc agcctgtcct tccttctctg
     6781 taactctccc cataagaaag acttcaccat tctccgtcag tacctttaca tctgacagtt
     6841 tcaccctgct gctcttcagt aactgtgccc ggatttttgt ggtcagccac gcatcagtag
     6901 ctgttttatc agcccgaagt ggttttcccg tccttatttc attccatacc tgcacagtcc
     6961 cctcaacacc atctgcaatc tgacgggctt tttcacctgc actacggtct ggtgcctgcc
     7021 ctgtcagcag tacagtgccc tggtatgctg tgacgatgat tcgcgcatcc cccctgagtt
     7081 gtgagtcctt cgacagcgca cggctgaccc ttgcttccag aatttcatca tcaatctgcg
     7141 ttcccgcact tcgggggtcc actgacactt tagtacccac agcagccgta ccgaccagcg
     7201 cagcggcaac acaaccctgt aacagcaaca gagtcgaaag aaaaggcaga aaccagcgtt
     7261 gcatggcaga atcctccacg gtctgatatc cggaatttcg cagtatgacg gcaggaatca
     7321 gccatcacag ataccacaag cttcagagag taacgaaaac gttaaaggaa acggaagtgc
     7381 ctgaatgtaa agaacgggga accacacagg gtatggacaa acataataac aataacagga
     7441 tgaaaatatt gaataaaaaa taaaaaccga gacatcatca tttgcagtaa tagccacctc
     7501 cttaccgaaa agtgtgagtt gccggacatt ttttaaacgc atgataaggt ctgatgatac
     7561 agtcaccctg caaccaggag gtacatcatg cgtatacgtg tttttggtta ccggcactgc
     7621 ccttcagtcc atcgcatcat gcatcacgca acgcgaatcc ccgatgaaat ggaaccggat
     7681 gttgtgttta ttgatgtcgc gaagcacagt gtgacgctgc gggatatggc tgaccgtgca
     7741 ggctgcccgg tggataactt cccgtgtgtg tttgttgatg atgtctatct ggagcccctc
     7801 caccagaacc gggcaaaccg cactttacat actgctgaat atttattacc ggatatggac
     7861 aatcgctttc attcgggata aaaaatactg cgcgttcctg gtttattttg cgttgctgtc
     7921 atgacgtgtt tgccttcatt gccgggatcg cgcacttttt gttataactc cccggaggac
     7981 actgtctgta atcagatgga aacctttatc tcagtgatgt cctggttaat acagaatact
     8041 gccccggacc aagtaacata atggcaatcc cccccatgaa atacagcatt tcattttcaa
     8101 gcccccacgc ccctacttca gtcagtgtga aaaacttccc tgttccgacc agaagcgtgg
     8161 caacaatcag tgtcagcgca taaatcagag cagcaggtct ggtaaatatc ccaagaatga
     8221 taagtaccgg agccacgatt tcaccaatat acacaccata ggcaataaat cctggcaaac
     8281 cagcatcctg caacattgat ataatccagt tgacaccgtg ctctattttg gccacaccat
     8341 ggaacagcat caatatacca aatgtcagtc tcagcaacag aagacctgta tccgggtagt
     8401 ctgtcagtct ggtaaagaac atatttattc ttgctggcat aaaaaacctc gttaaacgat
     8461 aacagataat gaagaaacta ttatcgttga gtcgacacca tcaattgacc agatatttct
     8521 gacattcata atcaaaaaat ttgacaaaac tgtaaacgtt cgctttttat accaacacag
     8581 aatccactca gtcacacaac ctgtatgcgt catttcctgc tgttatttaa aaaatttctc
     8641 acgtaacaga catttcaaaa aaacactaaa aaataccaga acaattaaga taatattacg
     8701 atcaaataag taaaagtctt ttgtctgtta tttttatttt ctgtgattac tgtcattctt
     8761 tcaccacttt aattaacatc gcataataat ccgggaggtg gataagatat gaaaattaac
     8821 tatcctttac tggcgctggc gttgaattat tacctgtaga gctcactccg gatgagcaga
     8881 aagaaccgat ggtggctctt actccacttt tatccactcc ttcacacagc actgtcagtg
     8941 caggtgcctg aaagatctcg ttccgtcacg gtaaaatgac gctggaaaat tcttcaccag
     9001 aactgctcac tgtgttaatt cgtgaactga cctgggggga atgcgtggta ccctgctgta
     9061 cggactaatc ggaacctgtc ggttgaacgg tatcgatccg gaagcgtatc tgcgccatat
     9121 cctgaacgtt ctaccggaat ggccttcctg catggttgat gaactcctgc catggaatat
     9181 ggtgctccca ttggggtaag cgtcaatacg gcgttcgctt aacttaacac ttactttcaa
     9241 agcctgatat ctgtcctacg cttttcctca ataccacatc gccagcagga aacaacctaa
     9301 tgcctgtgtt ggggtctaaa ttactaatga taatcatttg cattgtgtat agcttatgct
     9361 ataaaacata gccgttgcta tgtttgttct aattttactc actataggta ctgaattatg
     9421 cactcaataa aaaaagtaac catgctcctg gggtgcctcg ccctcacctg ctcgatagca
     9481 tttcaggcga gtgcaactga aaaattcaag gtcattacaa catttaccat catcgcagat
     9541 atggccaaaa acgtggctgg agatgctgca gaagtctcat ccataaccag gcccggtgca
     9601 gaaattcatg agtatcagcc tacccctggc gatattaaac gtgcgcaggg ggcacaactg
     9661 attctcgcca acggtatgaa tctggaattg tggttccagc gcttttacca gc
//
LOCUS       DAMZFT010000174         9628 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.174, whole genome shotgun sequence.
ACCESSION   DAMZFT010000174 DAMZFT010000000
VERSION     DAMZFT010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9628)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9628)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9628
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.174"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..98
                     /locus_tag="PN977_004500"
     CDS             <1..98
                     /locus_tag="PN977_004500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="protein disulfide oxidoreductase"
                     /protein_id="HDI6044450.1"
                     /translation="VVVSKGQVVTTTSGWTSYWGMKLRLWRAAMF"
     gene            281..1096
                     /locus_tag="PN977_004501"
     CDS             281..1096
                     /locus_tag="PN977_004501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014640325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)-translocating NADH-quinone reductase
                     subunit C"
                     /protein_id="HDI6044451.1"
                     /translation="MIRSGDFFDFHFDRDNNSMRKGKIIVASMMVLCVLIFVAAAAWF
                     MLFQGEMTEPTEEEKQAAILHAAGLMKSETQDKKSVETLYHRYIIQRHVNLDSGELVA
                     GSSADTARQKCEKLAPERDPAQVRQRCTVADVFFVKDKNNEIQQVIIPVTGKGAKSMM
                     HAFLALGLDGRTVRNLYYYQQRETPFLGARVEDANWRKQWPGKRLLDNSGHPALKIVQ
                     DKPEHADEYTVDGISGATLTSTGVEKSINYWMGPQGYGQFLQRLASDRNNLNL"
     gene            1443..3329
                     /locus_tag="PN977_004502"
     CDS             1443..3329
                     /locus_tag="PN977_004502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012132915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FTR1 family iron permease"
                     /protein_id="HDI6044452.1"
                     /translation="MSVWASTNYAPLIEDIEQRLDKTAELYQQQHADEARRTVQMAYF
                     EVFENLEGPIRINISARKSYEMESAFGEIRRMIGEKKPLADVQARIDWLKAALREVEP
                     VLDGGHRLVAEEQHNALSRDDIAVHWQESFRTIDDLLAQAVTEYQAGNYSVASQHVQQ
                     AHYQGFKNSEMEMSLRQNRSAKDAASINQQFSSLIALTAQPDHLNDVSYQVTTLLQDI
                     EDILPGLPTTRDDQQVAAAQSADNIPAADEGNNRTDWAEVTTGINQSIQDAIARYQGG
                     DAKNAILDIQDTYFDRFEASGMENKIGSRDSAFKTTLEAYFTRLVSLMKAGQPVERLN
                     AEASALTQDLQKAVTMLGEGEETQWSLLLYSLMIIVREGLEALLIVAAIVAYMVKNNH
                     QDKLPLIRQSVIVALIASVITAAIFQMLFTNSGASRELLEGITMLIAVVMLFFMSYWL
                     LSKVEARHWKAWLEGKLSHSLSRGSLVGLWLTSFLAVYREGAETVLFYYALIGDANTV
                     SGHMAIGAGFVIGCVVLLLAWLVMRYSVVRLPLKPFFMFTGSFMYLMAFVFAGKGVLE
                     LVEGKLFQPTLINGFPEISWLGIYPYVETLLPQVVLLLAALVALWVMRRKSAVPGETI
                     KNNP"
     gene            3370..3897
                     /locus_tag="PN977_004503"
     CDS             3370..3897
                     /locus_tag="PN977_004503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016674077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron transporter"
                     /protein_id="HDI6044453.1"
                     /translation="MTMKKTLIASAVMASIFIAPAAFAFKEYPAGEPVTMNEMELAAV
                     YLQPIDMEPRGMGLPAAKADVHLEADIHAVEGNKNGFGAGEWIPYLTISYTLVNNDTG
                     EKQEGTFMPMVASDGPHYGANIKMMGVGNYKVTYHIEPPSKAGMHRHTDSETGVGRWW
                     KPFDVSYEFKYVGLN"
     gene            4001..5380
                     /locus_tag="PN977_004504"
     CDS             4001..5380
                     /locus_tag="PN977_004504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003836820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2318 domain-containing protein"
                     /protein_id="HDI6044454.1"
                     /translation="MSYFFVTTLQVFFCIALLSGVLWSRNDPPSLRPLTWTLLTGLIA
                     GVLAGLFIHGSQPVQLLLVGAEVMVSLLFVLSFWWASTRIRYLWQGILIFGAARHWAL
                     DPNLGGLTSTHVLNTDLLLNLTAVVLAFAILCLAGVLCAMLLRRIRGLYWPLTLILLV
                     MIWLPLSGNLLLLLMKLQVVPLGKSLLSFVAKVTNNTALYNWAGAALLLALALCWLPA
                     LLRAFRQTRETEEPIAHRLALAQRRNALRLWLVTIGCAVVVIAGQLWWDKVASQPPQL
                     SEAVPVTLGSDGMVRLPVEQLRDGKLHRFVWVADDGKAVRFFVINRYPDKLRFGVVFD
                     ACLLCGDQGYVMEGNQVICVACGVHIFIPSIGKAGGCNPVPIENWHNDEKELVIPGKE
                     LATGVNYFSTVMTIKVTDPVDGSTLTNTSADYKYSYGGKTWFFSSEANYERFRETPEQ
                     FVPADMREE"
     gene            5383..6666
                     /locus_tag="PN977_004505"
     CDS             5383..6666
                     /locus_tag="PN977_004505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011906231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDI6044455.1"
                     /translation="MLWRMLRQSWGRNLRRKVLAIITVFLASSLISALLAVSIDIGDK
                     MSRELKSYGANILIEPAGQAALPALFSESSNPLSGQDFLDEAELPNIKDIFWRNNIVG
                     FAPMLGGEASVEGEPVRILGTFFSQPVDIPDEEGYETGQKTVSPYWQVTGDWPQEPAG
                     AEPQTLVGHALARQMSWKPGDKLTLRTEGEAVQVTVSGILSSGGDEDNQLVMPLSTVQ
                     HLLGLPGKVQAIRVSALTVPENELSRRARENLDALNAEEYDLWYCTAYVSSIAHQLEE
                     AISGAEVRPVWQVAASEGVVIDKIQLLMAVVTVAALVASAMGIASLMTSTIMERAKEI
                     GLMKALGARQWQIMLLFYLEAASSGLAGGALGCIAGWGLAKAIGVMLFDAPLNFAWIV
                     VPCVLVIAVLIALIGTWFPARRIARLYPVEVLYGR"
     gene            6656..7786
                     /locus_tag="PN977_004506"
     CDS             6656..7786
                     /locus_tag="PN977_004506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000729219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDI6044456.1"
                     /translation="MAAKRTMLWLLVWRALRLRFQRVSVVFAALMVGATIVTALSAVW
                     FDINTKMSEELRTFGANFYIGPGHGSSMPQQELQSILDQAPQGLVHGASPWLYGMART
                     ELEKVVMVGVWFESLQKMVPYWQVTGSWIGVSFDDRNAMIGVKLAERLNVQPGDSITL
                     VDHNQRKNLQIKGIVESGDATDNMLIVSLDVAQAWLHQPGKISHGLLSVSNDVGQVEN
                     YASRLQAQYPDLEIRPVRKVSASEGQVLDKIKGLMGLVSIVILALSSLCVNTTLMAIV
                     GERAREFALQKALGSSNGDIVRQILLETSIIALAAVACGWVLGYLLAQLLGLTVFNAA
                     ISLRLPVLPITLVLSLLVAILAAIVPVRRAVSVEPAKVLKGE"
     gene            7791..8486
                     /locus_tag="PN977_004507"
     CDS             7791..8486
                     /locus_tag="PN977_004507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004863043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDI6044457.1"
                     /translation="MSVTHIPQVAIETRHLYKRFGDVTALDDINLRIAQGEFVAIMGA
                     SGSGKTTLMNILTCLDTATEGQVFLDGTDAAALDEEGRRRFRAEKIGLVFQQFHLIPF
                     LTALENIMLAQHYHSVVDEAAARKVLEQVGLGHRVTHLPSQLSGGEQQRVCIARALVN
                     EPPVIFADEPTGNLDEENEQRVLDLLTDLHRQGRTIVMVTHNPALGQFADRILRLQHG
                     KYLGEEANQHALA"
     gene            8473..8958
                     /locus_tag="PN977_004508"
     CDS             8473..8958
                     /locus_tag="PN977_004508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HDI6044458.1"
                     /translation="MRWHNVLLLAAAILISGCKEEKLAVGAPAPALAVFDLQGQESGL
                     ERWQGKAVYLNFWSAGCGGCLAEMDTLEALSKKWGDKVVVVAVNTDPETVRINDLLAK
                     HQITYPVVRDQLNITQERYQVIGTPTSVLIDSEGRVLELHQGMRKPPELEATFARLAA
                     R"
     gene            8983..9468
                     /locus_tag="PN977_004509"
     CDS             8983..9468
                     /locus_tag="PN977_004509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6044459.1"
                     /translation="MAFIWNDESLAILRENAGILTTEQIAQLLHTNITAVRNMAYRLK
                     LSLRVTAYNHRRIAQVQALYASETLSLKEIAAKTGLTASTVQYIVYVKSKNKPYATTE
                     YVSFETENAVHYRVQKEFVDTERSLLDNISDNTRFRELYLTDGTFYCARNIKYEVFIS
                     E"
BASE COUNT         2111 a         2376 c         2902 g         2239 t
ORIGIN      
        1 tggtggtggt gtcgaagggg caggtggtaa ctacgaccag cggctggacc agctactggg
       61 gcatgaagtt acgtctgtgg cgggcagcca tgttttgata gcgtgccggg gaaacccggc
      121 attttttaac ccattgtaag gatgtgtaaa acgagcggag agcgtgtttt ctgttatttt
      181 tgctgctttt ttaccgacgc cagttattat tttttaccgc tgcctgacgt tgcaaacctg
      241 aattgataat ggcatatctg ttttaattga atgaaagtct gtgatacgtt caggcgattt
      301 tttcgatttc cattttgaca gggataacaa ttcaatgcgc aagggtaaaa ttattgttgc
      361 atcaatgatg gtgctttgtg tccttatttt tgttgcggcg gcggcatggt ttatgctgtt
      421 ccagggagaa atgacggagc cgacagagga ggaaaaacag gcggcaattt tacacgctgc
      481 gggcctgatg aaatcagaga cccaggataa aaaatcagtc gaaacgctct atcatcgtta
      541 tattattcaa cgccatgtta atttagattc cggcgaactg gtggctggca gcagcgcgga
      601 caccgcgcgg cagaagtgtg aaaaacttgc ccctgaacgc gacccagcgc aggttcgtca
      661 gcgctgtacg gtggcggatg tgttctttgt gaaagataaa aataatgaaa ttcagcaggt
      721 tatcattcca gttaccggta agggtgcgaa atccatgatg catgcgtttc ttgctctggg
      781 gctggacggt cgtacggtga gaaatctgta ttattatcag caacgggaaa cgccgtttct
      841 gggggctagg gtggaagacg ccaactggcg caaacagtgg ccagggaaac gattattaga
      901 taacagcgga catcctgcat tgaaaatagt acaggataaa cctgaacatg ccgatgaata
      961 taccgttgac ggtatttccg gtgcgacgtt gacatcaacc ggcgttgaga aaagtattaa
     1021 ctactggatg gggccgcagg gatatggtca attcttacag cgtcttgcca gcgatcgaaa
     1081 taaccttaat ctttgaatat tgctgtacgc aataacgcgt tttatttctg cctgccgata
     1141 atgaataata tcactgtcgg cagggggatt tattcctctg ttaccgcgcc tgccttgccg
     1201 ttaattccat gagaaaaaca gggagataac gtccctttta tcaacgcata atgtgtgtgt
     1261 tcatcacgat ttgatgaaat aagtttacat aacttgtcgt ctgctgacat ttttatgatc
     1321 caggtcatgt ataacgcgac aaaaagatat gataataatt atcatttgta ttctttggcg
     1381 gtgtgcacgt gcgtaacttc cgtaactctt tgttggttac tctctttggc ttgtttataa
     1441 gcatgagtgt ctgggcgtcg acgaattatg cgcctttgat tgaagatatc gagcaacgtc
     1501 ttgataaaac agcggaactt tatcagcaac agcatgccga cgaggcgcgc cgcacggtac
     1561 aaatggccta ttttgaggtg ttcgagaacc tggaagggcc gatccgcatc aacatttccg
     1621 cgcgcaaaag ttacgagatg gagagcgcat tcggcgagat ccgtcgaatg attggcgaga
     1681 aaaaaccgct cgctgacgtc caggcgcgca ttgactggct gaaagccgca ctgcgcgaag
     1741 tggaaccggt gctggatggc ggacatcgtc tggtggccga agagcagcac aacgcgctgt
     1801 ccagggacga cattgccgtt cactggcagg agagtttcag gacgatcgac gatctgctgg
     1861 cgcaggcggt cactgaatat caggccggga attacagcgt tgccagtcag cacgttcaac
     1921 aggcgcacta tcagggcttt aaaaactctg agatggagat gtccctcaga caaaaccgtt
     1981 ctgcaaaaga tgccgcctcc attaaccaac aattctcatc cctgattgcg ctgaccgcgc
     2041 aacctgacca tctcaacgat gtctcctatc aggtcactac gctgctacag gatatcgagg
     2101 atatcttacc cggcttgcca accacccgtg acgatcagca ggttgccgca gcgcaaagcg
     2161 cagacaacat tccggcggcg gacgagggaa ataaccgcac cgactgggct gaggtgacga
     2221 cgggaattaa ccagagcatt caggacgcga tagcgcgcta tcagggcggc gatgcgaaga
     2281 atgcgattct ggatatccag gatacctact tcgaccgctt cgaagccagc ggcatggaaa
     2341 acaaaatcgg ctctcgcgat tcggcattta aaaccacgct ggaggcgtat tttacccgcc
     2401 tggtcagtct gatgaaggcc ggtcagccgg ttgagcgcct gaatgcagag gccagcgcgc
     2461 tgacgcagga tctgcaaaaa gccgtgacga tgctgggcga aggcgaagaa acccagtgga
     2521 gtctgctgct ctatagtctg atgatcatcg tgcgcgaggg gctggaagcg ctgctgattg
     2581 ttgcggcaat cgtggcctac atggtgaaaa acaaccatca ggataagctg ccgcttattc
     2641 gtcagtcggt aattgtggcg ctgatcgcca gcgtcatcac cgccgctatc ttccagatgc
     2701 tgtttactaa ctccggcgcc agccgtgaac tgctggaagg cataacgatg ctgattgccg
     2761 tcgtgatgct gttctttatg agttactggc tgctgtccaa agtggaagcg cgccactgga
     2821 aagcctggct ggaaggcaaa ctgtctcatt ccctgtcgcg aggctccctg gtcggactat
     2881 ggctgaccag cttcctggcg gtgtaccgcg aaggggcgga aacggtgctg ttctattacg
     2941 ctctgattgg cgatgcaaac acggtatccg gccatatggc gattggcgcc ggttttgtga
     3001 ttggctgcgt ggtgctgctg ctcgcctggc tggtgatgcg ttactcagtg gtgcgcctgc
     3061 cgctgaaacc cttctttatg tttaccggca gtttcatgta tctgatggcc ttcgtctttg
     3121 cgggcaaagg cgttctggaa ctggtggaag gcaaactgtt ccagcctacg ttgattaacg
     3181 gcttccctga aatcagctgg ctggggattt atccctacgt ggaaacgttg ttaccgcagg
     3241 tcgtattgct gctcgcggcg ctggtggcgc tgtgggtgat gcggcgcaaa agcgcggttc
     3301 ccggggagac gataaaaaat aacccataat cgttgtatag ccgttgtttt agacgagagg
     3361 atacgtttga tgaccatgaa gaaaaccctg attgccagcg cagtgatggc cagcattttc
     3421 attgcgccag ccgcgtttgc ttttaaagag tacccggcag gcgagcctgt caccatgaat
     3481 gaaatggagc tggcggccgt ttacctgcaa ccgattgata tggaaccgcg cggcatgggc
     3541 ttaccggcag caaaagccga tgttcacctt gaagcggata tccacgctgt agaaggcaat
     3601 aaaaacggct ttggcgcagg ggaatggatc ccgtacctga ccatcagcta caccctggtc
     3661 aacaacgaca cgggcgaaaa acaggaaggc accttcatgc cgatggttgc cagcgatggc
     3721 ccgcactatg gcgcgaacat caaaatgatg ggcgtgggca actataaagt gacctaccac
     3781 attgaaccgc cgtcaaaagc cgggatgcat cgccatactg acagcgaaac aggcgtaggc
     3841 cgctggtgga aaccgtttga tgtgagctac gagtttaaat atgtcggtct gaactaaaac
     3901 cgactaacca acgcgccagg aggattcccc cttctggcgc gttggtcaga ccgatcatat
     3961 gcccgtcgct taattcaacc cgccgtacag agtcgaagtt atgagttact ttttcgtcac
     4021 gacgctacag gtctttttct gcattgcgct tctttccggt gttctctgga gtcgaaacga
     4081 tcctccctct ttacgtccgc tgacctggac gctgctgacc gggctgattg ccggcgtgct
     4141 ggcgggactg tttattcacg gcagccaacc ggtacaactg ctgctggttg gggcggaggt
     4201 catggtatcg ctgctgtttg tcctcagttt ctggtgggct tcaacgcgta tccgctatct
     4261 gtggcagggg attctgattt tcggcgctgc ccgccattgg gcgctggacc ctaatctggg
     4321 cgggctgacc agtacgcatg tgctgaatac tgacctgctg ttaaacctga cggccgtcgt
     4381 tctcgctttt gcgatcctct gtctggcggg cgtactctgc gcgatgctgc tgcggcgtat
     4441 tcgcggcctg tactggccgt taacgctgat tctgctggtg atgatctggc tgccgctgag
     4501 cggcaacctg ctgttgttgc tgatgaaatt acaggttgtt ccgctgggaa aatcactgct
     4561 gagcttcgtg gcgaaagtca caaataacac ggcgctgtat aactgggcgg gagccgcgct
     4621 gctgctggcc ctggcgctgt gctggcttcc ggcgctgttg cgggcttttc gacagacgcg
     4681 ggaaaccgaa gaaccgattg ctcaccgtct ggcgctggcg cagcgtcgca acgctttgcg
     4741 gctgtggttg gtcacaatcg gctgcgcggt ggtcgttatc gcaggccaac tgtggtggga
     4801 taaagtggcc tcgcagccgc cgcaactctc tgaagccgtg cccgtaacgc tggggagcga
     4861 tggcatggtg cgcctgccgg ttgaacagct tcgtgacggt aaactgcatc gtttcgtctg
     4921 ggtggcggac gatggcaaag cggtgcgctt cttcgtgatt aaccgctatc ccgacaagct
     4981 gcgttttggc gtggtgttcg acgcctgcct gctgtgcggc gatcagggtt atgtcatgga
     5041 gggcaatcag gttatctgcg tcgcctgcgg agtgcatatt tttattccgt ctatcggcaa
     5101 ggccggtggc tgtaacccgg tgccgattga aaactggcac aatgatgaaa aagagctggt
     5161 gatccccggt aaagaactgg cgacaggggt gaactacttc tcgacggtca tgactattaa
     5221 agtgaccgat ccggtcgatg gttcaacgtt gaccaatacc tcagccgatt acaaatacag
     5281 ctatggcggc aagacctggt tcttctcgtc agaggctaac tatgaacgct tccgtgaaac
     5341 gccggagcag tttgttcctg ccgacatgag ggaggaataa tcatgctgtg gcggatgtta
     5401 cgacaatcct gggggcgcaa tttacgccgc aaagtgctgg cgataatcac cgtttttctg
     5461 gcctccagcc tgatctcggc gctgctggcg gtgtccattg atatcggcga caaaatgtcc
     5521 agagagctga agtcttatgg tgcgaacatt ttgattgaac ccgccggaca ggctgcgctg
     5581 cccgcgctgt ttagtgagag cagtaatccg ctttccggtc aggattttct tgatgaagcc
     5641 gagctgccga acatcaaaga tatcttctgg cgaaacaaca ttgtcgggtt tgcgccgatg
     5701 ctcggcggcg aagccagcgt ggagggagaa ccagtgcgga ttctgggcac tttcttcagt
     5761 cagccagtgg atattcctga tgaagaggga tatgagacgg ggcagaaaac ggtcagccca
     5821 tactggcagg tgaccggcga ctggccgcag gaacccgcag gcgcagagcc gcagacctta
     5881 gtcgggcacg cgctggcgcg gcaaatgagc tggaaacccg gtgataagct gacattacgt
     5941 accgaaggcg aagcagtgca ggtcaccgtc agcggtatcc tctccagcgg cggcgatgaa
     6001 gataaccaac tggtgatgcc gctaagcacg gtacagcacc tgctggggct ccccggaaaa
     6061 gtgcaggcga ttcgcgtctc ggcgctgacg gtgccggaaa acgaactgtc ccgccgcgcg
     6121 cgggaaaacc tggacgcgct caatgccgaa gagtacgacc tctggtattg cacggcctat
     6181 gtgtcgtcga tcgcgcatca gctggaagag gcgatttccg gtgcggaagt gcgcccggta
     6241 tggcaggtcg cggcgtccga aggggtggtg atcgataaaa ttcagttgct gatggcggtc
     6301 gttaccgtcg cggcgctggt ggcttccgca atggggatcg cctcgctgat gaccagcacc
     6361 attatggaac gcgccaaaga gatcggtttg atgaaagcgc tgggtgcgcg acagtggcaa
     6421 attatgctgc tgttctacct ggaagcggcg tccagcgggc tggctggcgg tgcgctggga
     6481 tgtattgccg ggtgggggct ggcgaaagcg attggcgtca tgctcttcga cgcgccgctt
     6541 aattttgcct ggattgtggt gccgtgcgta ctggtgattg cggtactgat tgcgttgatc
     6601 ggcacctggt tcccggcccg gcggattgcc cggctgtatc ctgtggaggt gctgtatggc
     6661 cgctaaacgt accatgctct ggctgctggt gtggcgggcg ctgcgtctgc gttttcagcg
     6721 ggtcagcgtg gtgtttgccg cgctgatggt gggggcgacc atcgtgacgg cgctttctgc
     6781 cgtctggttc gacattaaca ccaaaatgag cgaagaactg cgcaccttcg gcgcgaattt
     6841 ttacatcggg ccggggcacg gttccagtat gccgcagcag gagttgcaga gcattctcga
     6901 tcaggcgccg caggggctgg tgcatggcgc cagtccgtgg ttgtacggca tggcgcgtac
     6961 cgagctggaa aaagtggtga tggttggcgt gtggttcgag tcgctgcaaa aaatggtgcc
     7021 gtactggcag gtcaccggta gctggattgg cgtcagtttt gacgaccgta atgcgatgat
     7081 cggcgtcaaa ctggcggaac gcctcaacgt gcagccgggc gacagcatca cgctggtgga
     7141 tcacaatcag cgtaagaatt tgcagattaa aggcattgtg gagtctggcg atgcgacgga
     7201 caacatgctg atcgtcagcc tcgacgtggc gcaggcctgg ctgcatcagc cgggcaaaat
     7261 cagccacggc ttgctgagcg tcagtaacga tgtcggccag gtggagaact atgccagccg
     7321 gttgcaggcg cagtatcctg acctggagat tcgtccggtg cgtaaggtct ctgcttccga
     7381 agggcaggtg ctggataaaa ttaaaggtct gatgggactg gtctcgatcg tgattctggc
     7441 gctctcttcg ctgtgcgtga ataccacgct gatggcgatt gtcggcgagc gggcgcgcga
     7501 gtttgcgctg caaaaagcgc ttggttccag caacggcgat atcgtgcggc aaattctgct
     7561 ggaaaccagc atcatcgcgc tggcggcggt ggcgtgcggc tgggtgctgg gatatttgct
     7621 ggcgcaactg ttgggattaa cggtgtttaa tgccgccata tcactgcgtc tgccggtgtt
     7681 gcccattact ctggtgttgt ctttactggt cgccattctg gcggcgattg ttccggtgcg
     7741 acgcgcggta agcgtcgagc ctgctaaagt gctgaaagga gagtagcgcc atgtccgtta
     7801 cgcatatccc tcaggtggcg atcgaaaccc gccacttata caaacggttc ggtgacgtta
     7861 ccgccctgga tgacattaat ctgcgcattg cgcagggtga atttgtcgcc attatgggcg
     7921 cttctggttc tggtaaaacc acgctgatga atattctgac gtgccttgat acggcgacgg
     7981 aagggcaggt gtttcttgat ggcaccgatg ccgccgcgct ggatgaagag gggcgccgtc
     8041 gctttcgcgc cgagaaaatc ggcctggtat ttcagcagtt ccatttgatc ccgttcttaa
     8101 ctgcgctgga aaacatcatg ctcgcccagc attaccacag cgtggtggat gaagcggcgg
     8161 cgaggaaggt gctggaacag gtagggcttg ggcatcgtgt gacgcacctg ccgagccagc
     8221 tttccggtgg tgagcagcag cgtgtgtgta tcgcccgggc gctggtcaac gaaccgccgg
     8281 ttattttcgc ggatgaaccg acgggcaacc tggatgaaga gaatgagcag cgggtgctgg
     8341 atctgctcac cgatctgcat cgtcaggggc gcacgattgt gatggtgacg cacaaccctg
     8401 cgcttgggca gtttgccgac cgtattctgc ggctgcaaca cggtaaatat ctgggtgagg
     8461 aggccaatca acatgcgctg gcataatgtg ctgttactcg cggcggcgat cctgataagc
     8521 gggtgtaaag aagaaaagct ggcggtcggg gcaccggccc cggcgctggc ggtgtttgat
     8581 ctgcaaggcc aggagtcagg ccttgaacgc tggcaaggaa aggcggtgta cctgaatttt
     8641 tggtctgcgg gatgcggggg gtgcctggcg gaaatggaca cgctggaagc gctgagcaaa
     8701 aaatggggcg ataaagtggt ggttgtggcg gtcaataccg accctgaaac ggtcaggatc
     8761 aacgatctgc tggcaaaaca tcagataacc tatccggtgg tgcgcgatca gctcaacatt
     8821 acccaggaac gttatcaggt gatcggcacg ccgacctccg tactgattga ctccgaagga
     8881 cgcgttctgg agctacacca gggtatgcgt aagccgcctg agttagaggc cacgtttgcg
     8941 cgcctggcgg cgcgatgaac gacgcgtacg gagagatacc caatggcatt tatctggaac
     9001 gacgaaagcc ttgcgattct gcgggaaaac gcgggcatcc tcacgacaga gcagatagcg
     9061 caactgcttc acactaatat taccgccgtg cggaatatgg cctatcggct gaaactgagc
     9121 cttcgggtga cggcgtataa tcaccgacgc atagcgcagg ttcaggcgct ttatgcctct
     9181 gagacgttga gtctcaaaga gatcgcggcg aaaacggggc tgacggccag taccgtgcaa
     9241 tatatcgtgt acgtgaaatc aaaaaataag ccctacgcca ccacggaata tgtctctttt
     9301 gaaacggaga acgccgttca ttaccgcgtg caaaaagaat ttgtcgatac ggaacgttcc
     9361 ctgctggaca acatctctga taacacgcga tttcgtgaac tgtatctgac ggacggcact
     9421 ttttattgcg cccgcaatat taagtatgag gtttttatct cggagtaacc ggaacacgct
     9481 gacctgccgg ttatcttctg gaatctcccg ttttgcgaaa ccgttcccgg aaaaatgtca
     9541 tatttctgtc acactctatg aggattgata acaacaagag gttgtaggga tgaaaaaatt
     9601 gatactcgcc gccgctgtgg caggggct
//
LOCUS       DAMZFT010000175         9614 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.175, whole genome shotgun sequence.
ACCESSION   DAMZFT010000175 DAMZFT010000000
VERSION     DAMZFT010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9614)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9614)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9614
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.175"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(138..322)
                     /locus_tag="PN977_004510"
                     /pseudo
     CDS             complement(138..322)
                     /locus_tag="PN977_004510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165315.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            423..596
                     /locus_tag="PN977_004511"
     CDS             423..596
                     /locus_tag="PN977_004511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6044460.1"
                     /translation="MSYFSFEIILIPVKNIIPIITVTLILNYLNNSERSLVKQILIED
                     EIIVCATYLIPDI"
     gene            598..942
                     /locus_tag="PN977_004512"
     CDS             598..942
                     /locus_tag="PN977_004512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415690.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper family protein"
                     /protein_id="HDI6044461.1"
                     /translation="MKKKILAFGLISALFCSTPAMADMNRTTKGALLGAGVGLLTGNG
                     VNGVLKGAAVGAGVGAVTEKGRDGKNARKGAKVGAAVGAVTGVLTGNGLEGAIKGAVI
                     GGTGGAILGKMK"
     gene            946..1281
                     /locus_tag="PN977_004513"
     CDS             946..1281
                     /locus_tag="PN977_004513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmgD family protein"
                     /protein_id="HDI6044462.1"
                     /translation="MMMKKFALLAGLFVFAPMTWAQDYNIKNGLPSETYITCAEANEM
                     AKTDSAQVAEIVAVMGNASVASRDLKIEQSPELSAKVVEKLNQVCAKDPQMLLITAID
                     DTMRAIGKK"
     gene            complement(1339..3660)
                     /locus_tag="PN977_004514"
     CDS             complement(1339..3660)
                     /locus_tag="PN977_004514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HDI6044463.1"
                     /translation="MQGNNTIVTTGDYSIGLLSQTNGNLNTDTIIRVNTDGSVNPHLS
                     DEGNTFIVTAGNHAVGVLACASPGSARACVSSLDEESTADTGSNENNAKAKLDMAKGE
                     ITTHGTESYAAYANGTVVKAGSKLDYTNASVTLTDVDITTHGDNAHAIAARQGTVSFN
                     QGEIHTTGPDAAIAKIYNGGKVTLKNTSAVAHQGAGIVLESSINGQEATVDILSGSSL
                     RSANEILYNKNETSNVTITDSEVSSAADVFINNIKGHLVIDASNSKITGSANLSTDDS
                     THTYLSLSDNSTWDIKTDSTVSKLTVDNSTVYISRADGKAFEPTRLTITENYVGNNGV
                     LHLRTELGDDNSATDKVVINGNTSGTTRVKVTNAGGSGAYTLNGIEIISVEGESNGEF
                     IKDSRIFAGAYEYSLTRGNTEATNKNWYLTNFQATSGGETNSGGSSAPTVAPTPVLRP
                     EAGSYVANLAAANTLFVMRLNDRAGETRYIDPVTEQERSSRLWLRQIGGHNAWRDSNG
                     QLRTTSHRYVSQLGADLLTGGFTDSDSWRLGVMAGYARDYNSTHSSVSDYRSKGSVRG
                     YSAGLYATWFADDISKKGAYIDAWAQYSWFKNSVKGDELAYESYSAKGATVSLEAGYG
                     FALNKSFGLEAAKYTWIFQPQAQAIWMGVDHNAHTEANGSRIENDANNNFQTRLGFRT
                     FIRTQEKNSGPHGDDFEPFVEMNWIHNSKDFAVSMNGVKVEQDGARNLGEIKLGVNGN
                     LNPSASVWGNVGVQLGDNGYNDTAMMVGLKYKF"
     gene            complement(4387..4605)
                     /locus_tag="PN977_004515"
     CDS             complement(4387..4605)
                     /locus_tag="PN977_004515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001065870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6044464.1"
                     /translation="MNNSNNLDYFTLYIIFSIAFMLITLLVILIAKPSTGLGEVLVTI
                     NLLNALVWLAINLVNRLRERLVSHRDQH"
     gene            complement(4737..6260)
                     /locus_tag="PN977_004516"
     CDS             complement(4737..6260)
                     /locus_tag="PN977_004516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HDI6044465.1"
                     /translation="MRNTLIPILVAICLFITGVAILNIQLWYSAKAEYLAGARYAANN
                     INHILEEASQATQTAVNIAGKECDLEEQYQLGTEAALKPHLRTIIILKQGTVWCTSLP
                     GNRVLLSRIPVFPDSNLLLAPAIDTVNRLPILLYQSQFADTRILVTISDQHIRGALNV
                     PLKGVRYVLRVADDIIGPTGDVMTLNGHYPYTEKVHSTKYHFTIIFNPPPLFSFYRLI
                     DKGFGILIFILLIACAAALLLDRYFNKSATPEEILRRAINNGEIVPFYQPVVNGREGT
                     LRGVEVLARWKQPHGGYISPAAFIPLAEKSGLIVPLTQSLMNQVARQMNAISSKLPEG
                     FHIGINFSASHIISPTFVDECLNYRDSFTRRDLNLVLEVTEREPLNVDESLVQRLNIL
                     HENGFVIALDDFGTGYSGLSYLHDLHIDYIKIDHSFVGRVNADPESTRILDCVLDLAR
                     KLSISIVAEGVETKEQLDYLNQNNITFQQGYYFYKPVTYIDLVKIILSKPKVKVVVE"
     gene            complement(6592..6840)
                     /locus_tag="PN977_004517"
     CDS             complement(6592..6840)
                     /locus_tag="PN977_004517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6044466.1"
                     /translation="MNNSIPERFILQCALFKNLEREVFMTHGYVDSYIIDQALRLRLK
                     DETSVILSDLYLQILQYIEMHKTTLTDIIINDRESVIS"
     gene            complement(6953..7219)
                     /gene="ariR"
                     /locus_tag="PN977_004518"
     CDS             complement(6953..7219)
                     /gene="ariR"
                     /locus_tag="PN977_004518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm/acid-resistance regulator AriR"
                     /protein_id="HDI6044467.1"
                     /translation="MLEDTTIHNAITDKALSSYFRSSGNLLEEESAVLGQAVTNLMLS
                     GDNVNNKNIILSLIHSLETTNDILKADVIRKTLEIVLRYTADDM"
     gene            complement(7248..7520)
                     /locus_tag="PN977_004519"
     CDS             complement(7248..7520)
                     /locus_tag="PN977_004519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component-system connector protein YmgA"
                     /protein_id="HDI6044468.1"
                     /translation="MKTSDNESIKYEITGQAVLHILRMKINFSLQTLIKQLLVMKSAE
                     ENAFRRDLIDSIIRDFSNSDTGGPNRRTAIADNKSMFNGKKINRIH"
     gene            complement(7563..7799)
                     /locus_tag="PN977_004520"
     CDS             complement(7563..7799)
                     /locus_tag="PN977_004520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component-system connector protein YcgZ"
                     /protein_id="HDI6044469.1"
                     /translation="MHQNSVTSDSAGAITRYFAKANLPTQQETLGEIVTEILKDGRNL
                     SRKSLCAKLLCRLEQATGEEEQKHYNALIGLLFE"
     gene            8113..9324
                     /gene="bluF"
                     /locus_tag="PN977_004521"
     CDS             8113..9324
                     /gene="bluF"
                     /locus_tag="PN977_004521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="blue light-responsive regulator BluF"
                     /protein_id="HDI6044470.1"
                     /translation="MLTTLIYRSHIRDDEPVKKIEEMVSIANRRNMQSDVTGILLFNG
                     SHFFQLLEGPEEQVKMIYRAICQDPRHYNIVELMCDYAPARRFGKAGMELFDLRLHER
                     DDVLQAVFDKGTSKFQLTYDDRALQFFRTFVLATEQATYFEIPAEDSWLFIADGSDKE
                     LDSCTLSPTINDHFAFHPIVDPLSRRIIAFEAIVQKNEDSPSAIAVGQRKDGEIYKAD
                     LKSKALAFAMAHALELGDKMISINLLPMTLVNEPDAVSFLLDEIKANALVPEQIIVEF
                     TESEVISRFDEFAEAIKSLKAAGISVAIDHFGAGFAGLLLLSRFQPDRIKISQELITN
                     VHKSGPRQAIIQAIIKCCTSLEIQVSAMGVTTPEEWMWLESAGIEMFQGDLFAKAKLN
                     GIPSVAWPEKK"
     gene            9529..>9614
                     /locus_tag="PN977_004522"
     CDS             9529..>9614
                     /locus_tag="PN977_004522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDI6044471.1"
                     /translation="MAYYSIGDVAERCGINPVTLRAWQRRYG"
BASE COUNT         2780 a         2099 c         1953 g         2782 t
ORIGIN      
        1 cccatgccat tggataaatg atagtagcgt ctaagtgacc ttagctctgc tgcaacatca
       61 gcccgcaggc accagaccag gggattgatc ctgaagagac agcgcaggtg tattgtgttc
      121 accgctcatc aaagacatca tgatgaaata atgatatccc gcataagaaa aggcattttt
      181 taaacgcagt gcgctgaagt gtagttggat aaaaaatcac tcttttctga taatactgtt
      241 gtacgcttat ttttcgacaa tgaggccgtc ggcaaaataa aatgactaat gtaatcattg
      301 ctgatgaata ttttctgctc acataaaaat cacacaataa tattcataaa tcagattatc
      361 ttacctttac aacattgtat taacactcaa tgacacttct tgaataaaaa tattgtcaat
      421 caatgagtta tttctcattt gagattattc ttattcccgt aaaaaacata attcctatca
      481 tcacagtcac gctcattttg aattatctga ataatagcga gcgttcttta gttaagcaga
      541 tcttaattga agacgaaatt attgtgtgtg caacttactt aataccggac atctaagttg
      601 aagaaaaaaa tacttgcctt cgggcttatc tcagcattgt tctgctccac gccagcgatg
      661 gcggatatga atcgcaccac gaaaggtgca ttgctaggcg caggcgttgg tttacttact
      721 ggcaatggtg ttaatggcgt actgaaaggt gcagctgttg gcgctggtgt tggtgcagta
      781 acagaaaaag gccgcgacgg taaaaatgca cgtaaaggtg caaaagtggg tgccgccgtt
      841 ggtgctgtca ctggcgttct gaccggcaat ggcctcgaag gggcgattaa aggtgccgtg
      901 attggcggta cgggtggtgc aattctgggt aaaatgaaat aaggaatgat gatgaaaaaa
      961 tttgctttac tggcgggtct gtttgttttc gcaccaatga cctgggcgca ggactacaat
     1021 attaaaaacg gtttgccgtc agaaacctat attacttgcg cagaagccaa cgaaatggca
     1081 aaaactgaca gcgcacaggt tgcagaaatt gttgcggtaa tgggtaatgc cagcgttgcc
     1141 agccgtgatt taaaaattga acaatctccg gaactgagcg ccaaagtggt agaaaaactg
     1201 aatcaggttt gcgctaaaga cccgcaaatg ctgttgatca cggcaattga tgacaccatg
     1261 cgtgctatcg gcaaaaaata atatttcata tgttaccgcg tcgatgaaat cgacgcggtt
     1321 ttttagctaa tggcgggatc agaacttata tttcaggccc accatcattg cggtgtcatt
     1381 gtagccatta tcacccagct gcacgcccac attaccccag acgctggccg acggattcag
     1441 attgccattt acgccaagtt taatttcccc cagattacgc gccccgtctt gttcgacttt
     1501 cacaccattc attgagacag caaaatcttt actgttatgg atccagttca tttcaacaaa
     1561 aggttcaaag tcgtcaccgt gaggaccgct gtttttctcc tgagtacgaa taaaggtgcg
     1621 gaaaccaagt cgggtctgga agttgttatt tgcgtcattc tcaatacgtg agccattggc
     1681 ttccgtgtgc gcattatgat cgacgcccat ccagatagcc tgtgcctgtg gctggaagat
     1741 ccacgtatat ttcgccgctt ccagaccaaa ggatttattc agggcaaagc cgtaacccgc
     1801 ttccagcgag acggttgcgc ctttcgcgct ataggattca taggctaact catccccttt
     1861 caccgagttt ttaaaccagc tatattgcgc ccaggcgtca atgtatgcgc cttttttact
     1921 gatgtcatcg gcaaaccagg tggcatacag ccctgcgcta tagcctctga cactcccttt
     1981 cgaacgataa tccgacacgc tggaatgagt tgagttgtag tcgcgggcat aaccagccat
     2041 cactcccaaa cgccaactgt cactgtcggt aaaaccaccg gttaacagat cggcccctaa
     2101 ctgcgagacg tagcgatgcg aggtcgttct caactgtccg ttgctgtcac gccaggcatt
     2161 atgcccgcca atttgacgta gccaaagtcg gcttgaacgc tcctgttcag ttacaggatc
     2221 gatgtagcgc gtttcacctg cacgatcgtt cagacgcata acaaaaagag tgttagcggc
     2281 tgccaggttg gcgacgtaac ttccagcttc ggggcgcagg acgggggtag gcgcaacagt
     2341 aggcgctgaa cttcctccgg agtttgtttc accgccgctc gttgcctgga agttagtcag
     2401 ataccagttt ttattggtcg cttcggtatt acctcgggtt aatgaatatt cgtaggcacc
     2461 ggcgaaaatc ctcgaatcct taataaattc tccatttgat tccccctcaa cgctgataat
     2521 ctctatccca tttaacgtgt aagccccgct gccgccagca ttggtaactt taactcgggt
     2581 tgttccagag gtatttccat taataacgac tttatccgta gctgaattat catcacccaa
     2641 ttcagttctg agatgcaata cgccattatt accaacgtaa ttttcagtta ttgttaatct
     2701 tgttggttcg aaagcctttc catctgcccg ggaaatatag accgtactat tgtcaaccgt
     2761 gagcttgctc accgttgagt cagttttaat atcccaggta ctattatctg aaagcgacag
     2821 ataggtatga gtgctatcat ctgttgaaag attggctgaa cccgttattt ttgaattaga
     2881 tgcatcgatg accaaatgac ctttaatatt attaataaaa acatctgcag ccgatgatac
     2941 ctcactatcc gtaatggtca cgttactcgt ctcattttta ttgtagagga tttcatttgc
     3001 tgaccgtagt gagctacctg ataaaatatc taccgttgct tcctgaccat ttatggaaga
     3061 ctccagcaca attccagccc cctgatgcgc gactgcagat gtatttttca gcgtcacctt
     3121 gccgccatta taaattttag caatcgcggc gtcaggaccc gttgtatgaa tttctccctg
     3181 gttaaatgaa acagtcccct ggcgagcagc gatggcatga gcattgtccc catgcgtggt
     3241 aatatcaaca tcggttaatg taacgctggc attggtataa tcaagcttgc tacccgcctt
     3301 gacgacagtg ccattagcgt aagcggcata actttctgtt ccgtgggttg ttatctcccc
     3361 ttttgccata tccagttttg ctttggcgtt attttcatta cttcctgtat cggcagtact
     3421 ttcttcatca agagaagata cacacgcacg cgcgcttccg ggagatgcac aagcaagaac
     3481 gccgactgcg tgattacccg cagtaacaat aaaagtatta ccttcgtcag ataaatgggg
     3541 gttaaccgaa ccgtcagtgt tgactcttat tatggtatca gtattcaggt ttccattcgt
     3601 ttgacttagc aaaccaattg agtaatcccc cgttgtgaca atcgtattat taccctgcaa
     3661 aataactgca ccgccatatt cacttacgac tgcgctagct gtatatactg tgcttagagg
     3721 actattgcca gaaggatata aaccacccgt tgttacaatg acgccattgt taatggttaa
     3781 attgctaccc tttcctgcaa cagatacacc gtaacttcca ttacccgttg ttataactag
     3841 cgcattatca atatctttta cttctccatc ttttatttca acaacatgtt taggttcatc
     3901 gtgctgaata aactatccag tttatgcatt acccaccgcc aaagcaataa gtcctaaact
     3961 aaacccgggg agttttttca gtttcataga ttaaccatcc attcattgga ttaacaaaca
     4021 ttcctggtca aataacctat gagttcagat taccaaaacg gagggtaaag acaaaaacaa
     4081 aagcacaaga aatttaaata gataaacagc gacaatgcat aaatgataaa taagtcagca
     4141 ttatcatttt tcaccttatt ttcatgacat tgatcacttt ggagtgacga ttttgtttaa
     4201 caaataactc ttcgacatca aaactattaa tagttgccat gagaattatt tcgatgatat
     4261 ataaaaacaa ctgtgataac accagggttg acaactattt gcgcaataat acctttcctt
     4321 caatgatcca ggcgttcact gccagcaacc cattcagccg catactgaca gtgagaaact
     4381 gaaagattag tgctgatccc tgtggctgac gagtctttcc cttaatcgat taaccagatt
     4441 gatcgccagc caaacaaggg cattaagcaa atttatcgtc acaagcactt ctcccagccc
     4501 ggtactgggt tttgcaataa ggatgaccag gagagtgatc agcataaatg caatggaaaa
     4561 gatgatatag agagtgaaat aatccagatt attactattg ttcatgctac taatggcaat
     4621 cctgtcaaaa ctttcaaaca attatagcaa acaaaataat gaacaatagt aacaagaggc
     4681 aacgagctaa ttagctgtct ataattacac cgcaccatgg ccttactgat caattttcac
     4741 tcaaccacaa ccttcacctt cggtttagaa aggataattt tgaccaggtc gatgtatgta
     4801 acaggtttat agaaataata accctgctga aatgtgatat tattttggtt cagataatca
     4861 agttgttctt tcgtttcgac accttcagcg acgatactga tcgaaagttt acgcgccaga
     4921 tccaatacac aatccagaat tcgggttgat tctgggtctg cgttgacgcg gccaacgaaa
     4981 ctgtgatcaa ttttgatata atcaatatgc aggtcatgca gataagaaag ccctgagtag
     5041 ccagtaccga aatcatccag cgcgatgaca aaaccatttt catgcagaat gttcaaccgc
     5101 tgaaccagac tttcatcaac atttaatggc tcacgctcgg tgacttccag aacgaggttt
     5161 aaatcgcggc gggtaaaact gtcacggtaa tttaagcact cgtcgacaaa cgtcggcgaa
     5221 ataatatgcg aggcgctaaa attaattcca atatgaaaac cctccggcag tttactcgag
     5281 atagcgttca tctgtctggc aacctgattc atcaggcttt gcgtaagcgg aacgattaat
     5341 ccggattttt cagcaagtgg aataaatgcc gcgggtgata tatatccacc gtgaggttgt
     5401 ttccagcggg ctaacacctc aactccccgc aatgtccctt cccgaccatt taccacaggt
     5461 tggtaaaaag ggacgatctc cccattattt atagcccgtc gcaggatctc ttcaggcgtt
     5521 gcgcttttat tgaaatacct atcaagcagc aaagcagcgg cgcaggcgat taacagaata
     5581 aatatcaata tcccaaagcc tttatcgata agtctgtaga agctaaagag tggtggcggg
     5641 ttaaagataa tagtgaaatg atattttgtg gagtgaacct tctcggtata gggataatgt
     5701 ccattaagcg tcatcacatc acccgtaggt ccaataatgt catccgccac gcgtaataca
     5761 taccttaccc ctttcaaggg tacattaagc gccccgcgaa tatgctgatc acttatcgta
     5821 accaaaatgc gcgtatctgc aaattggctc tgatagagca ggataggtaa tctattaacg
     5881 gtgtcgattg ccggagccaa cagtaaatta ctgtccggga aaacaggaat acgagacagc
     5941 aggacccgat tccccggtaa ggatgtacac cagaccgttc cctgtttgag aatgatgatt
     6001 gtgcgcaggt gaggtttcag agctgcttca gtgccaagct gatattgctc ctcgaggtcg
     6061 cattccttcc cggcaatgtt aaccgctgtt tgagtcgcct gtgacgcttc ttcaagtata
     6121 tgattgatat tgttggcggc atatctcgct cctgccaggt actctgcttt tgcggaatac
     6181 cagagctgga tgttcaaaat agcgacaccg gttataaaca agcatatcgc aacgaggatg
     6241 ggtatgagtg tattgcgcat ttgcttgtcc cggccaaagg gtttaataca tgaaaatgcc
     6301 attcattcgc tcgcgctggc atctacagag agcatggtag agagcataag aaatatccac
     6361 gccgaagata aaattttctc catcttgatc taacccatta attattgagc ataatgtaga
     6421 taggataaaa taaattaggc gggatatcag gtgtcaagaa tggagagcga gaactctcca
     6481 ttcttggcac ctgatattgc ggacatatta agaaagcata acgcctgaaa tgctcacttt
     6541 gcaccagcat ggtaatacag ctgatgttta ttctataacc ttactcaagt tctaagagat
     6601 cacggattcc ctgtcattaa taatgatatc cgttagtgta gtcttatgca tttcaatgta
     6661 ctgcaatatt tgcagataga gatcagaaag aatcacactg gtttcatctt ttaaacgcaa
     6721 gcgtaaagcc tgatcaataa tataactatc aacataccca tgcgtcatga acacctccct
     6781 ctcgagattt ttaaacaaag cacattgcaa aataaatctc tctgggattg aattattcat
     6841 gtcagcctcg tagaaatatt aacccaataa aattatgctg tgtatagtct aaagcgacag
     6901 acaaaaataa gatgaatgag ttttctataa atttatactt aataattaaa agtcacatat
     6961 catccgctgt gtatcgcaac acgatttcca gtgtttttct gatcacatca gctttgagga
     7021 tatcatttgt tgtttccagg gagtgtatca gacttaagat aatattttta ttattaacat
     7081 tatcgcctga aagcattaaa ttggtgacag cctgccctaa cactgctgac tcttcttcta
     7141 acaaattacc cgaactgcga aagtaacttg ataacgcttt atcagttatt gcattatgaa
     7201 ttgtagtatc ttcaagcatg gtaacctctc atcttactta tgaaatttta atgtattctg
     7261 tttattttct taccattgaa catactttta ttgtcagcta tcgctgttct tctgtttggc
     7321 cctcctgtat cactgttgct aaaatcacga ataatgctgt caattaaatc tcgtcggaaa
     7381 gcattttcct cagccgattt cattacgagt aattgcttaa taagagtctg caacgaaaaa
     7441 tttatcttca tgcgcagtat atggagcacc gcctggccag taatttcata ttttatactt
     7501 tcattatcag atgtcttcat aacgacttca tagctaacct gttaaataat caggctgttg
     7561 cattattcaa aaagcaaccc aattagtgca ttataatgtt tctgttcctc ttcccctgtc
     7621 gcttgttcca gtcggcaaag aagtttggca caaagcgact ttcgactcag attacgtcca
     7681 tctttcaaaa tctcagtcac aatttccccg agagtttcct gctgagtagg caagttagct
     7741 tttgcaaagt aacgagtgat tgctcccgca gaatctgaag tcactgaatt ttgatgcatg
     7801 ctacgcctct gttaaaaatg ttaatcaatg ttaagtgagg taacaatagc aaacttgtac
     7861 gaaatatgtg tacagaatga taaattaatt ttttgcattt agtgctaata catacaaaac
     7921 aacatgttat gataaaaaat attgcatcat ttagataaca acctatacaa gaattttcgc
     7981 caactcatct tcaacttttt ccatttttta tcaatgtaaa gaaacagtta tgttttggta
     8041 ttaaaagaaa gacaccggaa gatatgtctg ttaccgtctt actctcgcct ctcccattac
     8101 cctggacagt taatgcttac cacccttatt tatcgtagcc atatacgtga cgacgaacct
     8161 gtcaaaaaaa tcgaagaaat ggtttcgata gcaaatcgca ggaacatgca gtctgacgta
     8221 acagggatct tactgtttaa tggttctcat tttttccagc ttctggaagg tccggaagaa
     8281 caggttaaaa tgatatatcg ggctatatgc caggatccac ggcactataa tattgttgag
     8341 ctgatgtgcg attacgcgcc tgctcgccgt tttggcaaag cgggaatgga attatttgat
     8401 ttgcgcctgc acgagcgaga tgacgtttta caggccgtat tcgacaaagg cacatcaaaa
     8461 tttcagctaa cttatgatga cagagcgcta caattttttc gtacttttgt ccttgcaacc
     8521 gaacaagcaa cctatttcga gatccctgcc gaagactcct ggctttttat cgctgacgga
     8581 tctgataaag aacttgattc ctgtaccctt tcaccaacta taaatgacca ctttgccttt
     8641 catcctattg tcgatccctt atcgcggcgg ataatcgctt ttgaagccat tgtgcaaaaa
     8701 aatgaagata gcccatcagc tatagcggtt gggcagcgta aagacgggga aatctacaaa
     8761 gcggatctca aaagtaaggc gcttgcattc gcgatggcac acgcacttga gctcggtgat
     8821 aaaatgattt caatcaatct attacctatg accctggtta acgaacctga cgcagtctct
     8881 tttttacttg atgaaataaa ggccaatgct ctggtgcctg aacaaatcat cgttgaattt
     8941 actgaaagtg aagtcatatc tcggtttgat gagtttgcag aagcgattaa atcgcttaag
     9001 gctgccggta tcagtgtagc aattgatcat tttggcgcag gttttgccgg tttgttactc
     9061 ctgtcacgct tccagcctga cagaattaaa atcagtcagg aattgattac caatgttcat
     9121 aaaagcgggc cacggcaggc aattattcaa gcgatcataa aatgctgtac atcacttgaa
     9181 attcaagtct ctgctatggg tgtgacaaca ccagaagagt ggatgtggct tgaatctgca
     9241 ggaattgaga tgtttcaggg agatctgttt gcgaaagcta aattgaatgg tatcccttca
     9301 gttgcgtggc cggagaaaaa ataattttca gcacattatt tcgtatgatt tctgaaaatc
     9361 atcgtgaaaa tgaatacgtt atgtttaaat ttaatccaca ttaaaaataa tgtttcattc
     9421 aatttagctg aaatcttatt tatactttgt acaattttag cgtatattgt acaaagtatt
     9481 attgggtcgt gtacaggcga cggagatttg tgaccgcaag gaggaattgt ggcttattac
     9541 agcattggtg atgttgctga acgttgcggg attaatcctg tcactctccg ggcctggcaa
     9601 cgccgctacg gttt
//
LOCUS       DAMZFT010000176         9534 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.176, whole genome shotgun sequence.
ACCESSION   DAMZFT010000176 DAMZFT010000000
VERSION     DAMZFT010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9534)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9534)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9534
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.176"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..1164
                     /gene="mglA"
                     /locus_tag="PN977_004523"
     CDS             <1..1164
                     /gene="mglA"
                     /locus_tag="PN977_004523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020883197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     ATP-binding protein MglA"
                     /protein_id="HDI6044472.1"
                     /translation="FVDQDKMYRETKAIFDELDIDIDPRARVGTLSVSQMQMIEIAKA
                     FSYNAKIVIMDEPTSSLTEKEVNHLFTIIRKLKERGCGIVYISHKMEEIFQLCDEVTV
                     LRDGQWIATEPLAGLTMDKIIAMMVGRSLNQRFPDKENKPGEVILEVRNLTSLRQPSI
                     RDVSFDLHKGEILGIAGLVGAKRTDIVETLFGIREKSAGTITLHGKKINNHNANEAIN
                     HGFALVTEERRSTGIYAYLDIGFNSLISNIRNYKNKVGLLDNSRMKSDTQWVIDSMRV
                     KTPGHRTQIGSLSGGNQQKVIIGRWLLTQPEILMLDEPTRGIDVGAKFEIYQLIAELA
                     KKGKGIIIISSEMPELLGITDRILVMSNGLVSGIVDTKTTTQNEILRLASLHL"
     gene            1180..2190
                     /gene="mglC"
                     /locus_tag="PN977_004524"
     CDS             1180..2190
                     /gene="mglC"
                     /locus_tag="PN977_004524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     permease MglC"
                     /protein_id="HDI6044473.1"
                     /translation="MSALNKKSFLTYLKEGGIYVVLLVLLAIIIFQDPTFLSLLNLSN
                     ILTQSSVRIIIALGVAGLIVTQGTDLSAGRQVGLAAVVAATLLQSMDNANKVFPEMAT
                     MPIALVILIVCAIGAVIGLINGLIIAYLNVTPFITTLGTMIIVYGINSLYYDFVGASP
                     ISGFDSGFSTFAQGFVALGSFRLSYITFYALIAVVFVWVLWNKTRFGKNIFAIGGNPE
                     AAKVSGVNVGLNLLMIYALSGVFYAFGGMLEAGRIGSATNNLGFMYELDAIAACVVGG
                     VSFSGGVGTVIGVVTGVIIFTVINYGLTYIGVNPYWQYIIKGAIIIFAVALDSLKYAR
                     KK"
     gene            complement(2261..3496)
                     /gene="preA"
                     /locus_tag="PN977_004525"
     CDS             complement(2261..3496)
                     /gene="preA"
                     /locus_tag="PN977_004525"
                     /EC_number="1.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreA"
                     /protein_id="HDI6044474.1"
                     /translation="MLTKDLSITFCGVKFPNPFCLSSSPVGNCYEMCAKAYDTGWGGV
                     VFKTIGFFIANEVSPRFDHLVKEDTGFIGFKNMEQIAEHPLEENLAALRRLKEDYPDK
                     VLIASIMGENEQQWEELARLVQEAGADMIECNFSCPQMTSHAMGSDVGQSPELVEKYC
                     RAVKRGSTLPMLAKMTPNIGDMCEVALAAKRGGADGIAAINTVKSITNIDLNQKIGMP
                     IVNGKSSISGYSGKAVKPIALRFIQQMRTHPELRDFPISGIGGIETWEDAAEFLLLGA
                     ATLQVTTGIMQYGYRIVEDMASGLSHYLTDQGFDSLQEMVGLANNNIVPAEDLDRSYI
                     VYPHINLDKCVGCGRCYISCYDGGHQAMEWSEKTRTPHCNTEKCVGCLLCGHVCPVGC
                     IDLGEVKFKKGEKEHPVTL"
     gene            complement(3490..4728)
                     /gene="preT"
                     /locus_tag="PN977_004526"
     CDS             complement(3490..4728)
                     /gene="preT"
                     /locus_tag="PN977_004526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreT"
                     /protein_id="HDI6044475.1"
                     /translation="MPQQNYLDELTPAFTPLLAIKEASRCLLCHDAPCSQACPAQTDP
                     GKFIRSIYFRNFKGAAETIRENNALGAVCARVCPTEKLCQSGCTRAGVDTPIDIGRLQ
                     RFVTDFEQQTGMEIYQPGTKTLGKVAIIGAGPAGLQASVTLTNQGYDVTIYEKEAQPG
                     GWLRNGIPQFRLPQSVLDAEIARIEKMGVTIKCNNEIGNTLTLEQLKAENRAVLVTVG
                     LSSGSGLPLFEHSDVEIAVDFLQRARQAQGDISIPQSALIIGGGDVAMDVASTLKVLG
                     CQAVTCVAREELDEFPASEKEFASARELGVSIIDGFTPVAVEGNKVTFKHVRLPGELT
                     MAADKIILAVGQHARLDAFAELEPQRNTINTQNYQTRDPQVFAAGDIVEGDKTVVYAV
                     KTGKEAAEAIHHYLEGACSC"
     gene            complement(4922..5161)
                     /locus_tag="PN977_004527"
     CDS             complement(4922..5161)
                     /locus_tag="PN977_004527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2542 family protein"
                     /protein_id="HDI6044476.1"
                     /translation="MDVQQFFVVAVFFLIPIFCFREAWKGWRAGAIDKRVKNAPEPVY
                     VWRAKNPGLFFAYMVAYIGFGILSIGMIVYLIFYR"
     gene            complement(5164..5883)
                     /gene="sanA"
                     /locus_tag="PN977_004528"
     CDS             complement(5164..5883)
                     /gene="sanA"
                     /locus_tag="PN977_004528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane permeability protein SanA"
                     /protein_id="HDI6044477.1"
                     /translation="MLKRVFLSLLVLIGLLLLTVLGLDRWMSWKTAPYIYDELQDLPY
                     RQVGVVLGTAKYYRTGVINQYYRYRIQGAINAYNSGKVNYLLLSGDNALQSYNEPMTM
                     RKDLIAAGVDPSDIVLDYAGFRTLDSIVRTRKVFDTNDFIIITQRFHCERALFIALHM
                     GIQAQCYAVPSPKDMLSVRIREFAARFGALADLYIFKREPRFLGPLVPIPAMHQVPED
                     AQGYPAVTPEQLLELQKKQGK"
     gene            complement(6033..6917)
                     /gene="cdd"
                     /locus_tag="PN977_004529"
     CDS             complement(6033..6917)
                     /gene="cdd"
                     /locus_tag="PN977_004529"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HDI6044478.1"
                     /translation="MHPRFQTAFAQLADNLQSALEPILADKYFPALLTGEQVSSLKSA
                     TGLDEDALAFALLPLAAACARTPLSNFNVGAIARGVSGTWYFGANMEFIGATMQQTVH
                     AEQSAISHAWLSGEKALAAITVNYTPCGHCRQFMNELNSGLDLRIHLPGREAHALRDY
                     LPDAFGPKDLEIKTLLMDEQDHGYALTGDALSQAAIAAANRSHMPYSKSPSGVALECK
                     DGRIFSGSYAENAAFNPTLPPLQGALILLNLKGYDYPDIQRAVLAEKADAPLIQWDAT
                     SATLKALGCHSIDRVLLA"
     gene            complement(7047..7742)
                     /locus_tag="PN977_004530"
     CDS             complement(7047..7742)
                     /locus_tag="PN977_004530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidB/LrgB family autolysis modulator"
                     /protein_id="HDI6044479.1"
                     /translation="MMANIWWSLPLTLIVFFAARKLAARYKFPLLNPLLVAMVVIIPF
                     LMLTGISYDSYFKGSEVLNDLLQPAVVALAYPLYEQLHQIRARWKSIITICFIGSVVA
                     MVTGTSVALLMGASPEIAASILPKSVTTPIAMAVGGSIGGIPAISAVCVIFVGILGAV
                     FGHTLLNAMRIRTKAARGLAMGTASHALGTARCAELDYQEGAFSSLALVLCGIITSLI
                     APFLFPIILAVMG"
     gene            complement(7739..8137)
                     /locus_tag="PN977_004531"
     CDS             complement(7739..8137)
                     /locus_tag="PN977_004531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HDI6044480.1"
                     /translation="MSKTLNIIWQYLRAFVLIYACLYAGIFIASLLPVTIPGSIIGML
                     ILFVLLALQILPAKWVNPGCYVLIRYMALLFVPIGVGVMQYFDLLRAQFGPVVVSCAV
                     STLVVFLVVSWSSQLVHGERKVVGQKGSEE"
     gene            8376..9323
                     /gene="dusC"
                     /locus_tag="PN977_004532"
     CDS             8376..9323
                     /gene="dusC"
                     /locus_tag="PN977_004532"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(16) synthase DusC"
                     /protein_id="HDI6044481.1"
                     /translation="MRVLLAPMEGVLDSLVRELLTEVNDYDLCITEFVRVVDQLLPVK
                     VFHRICPELQNASRTPSGTLVRVQLLGQFPQWLAENAARAVELGSWGVDLNCGCPSKT
                     VNGSGGGATLLKDPELIYQGAKAMREAVPAHLPVSVKVRLGWDSGEKKFEIADAVQQA
                     GATELVVHGRTKEQGYRAEHIDWQAIGEIRQRLNIPVIANGEIWNWQSAQQCMTISGC
                     DAVMIGRGALNIPNLSRVVKYNEPRMPWPEVVALLQKYTRLEKQGDTGLYHVARIKQW
                     LSYLRKEYDEATELFQHVRMLNNSPDIARAIQAIDIEKL"
BASE COUNT         2437 a         2368 c         2395 g         2334 t
ORIGIN      
        1 tttgtcgatc aggacaaaat gtaccgcgaa accaaagcga tttttgatga actggatatt
       61 gatatcgatc cgcgtgcgcg cgtcggcaca ttatccgttt cgcaaatgca gatgatcgaa
      121 atcgccaaag cgttttccta taacgcgaaa attgtgatta tggatgaacc gacttcttcg
      181 ttaaccgaaa aagaggtcaa tcatctgttc actattattc gtaaattaaa agagcgcggc
      241 tgcggtattg tttatatctc acataaaatg gaagaaatct tccagttatg tgatgaagtt
      301 accgtattgc gcgacggtca gtggatcgcc accgaaccgc tggcagggct gacgatggac
      361 aagatcatcg ccatgatggt tgggcgttct cttaaccagc gtttccctga caaagaaaac
      421 aagccgggcg aagtgattct ggaagtgcgt aacctgacgt cgctgcgcca gccgtcgatc
      481 cgtgatgtct cgtttgatct gcataaaggg gagattctcg gtatagccgg tctggtggga
      541 gcgaaacgta ccgatattgt tgagacgtta tttggtattc gcgagaaatc ggctggcacc
      601 attacgttgc acggcaaaaa gatcaataac cataatgcca acgaagccat aaaccacgga
      661 tttgcactgg taactgaaga gcgccgctca acgggaattt atgcctatct ggatattggc
      721 tttaactcgt taatttccaa tattcgcaat tacaaaaaca aagttggctt gctggataat
      781 tcgcggatga aaagcgatac ccagtgggtc attgattcga tgcgggtaaa aacgccaggt
      841 catcggacgc aaataggttc gctctccggt ggtaatcagc aaaaagtgat tattggtcgc
      901 tggctgttaa cgcaaccaga aatattaatg ctcgatgaac cgacgcgcgg tattgacgtt
      961 ggggctaagt ttgagattta tcagttaatt gccgaactgg cgaagaaagg caaggggatt
     1021 attattatct cctctgaaat gcctgagttg ttagggataa cagaccgtat tctggtcatg
     1081 agcaatggtc tcgtttccgg aattgtcgat acaaaaacaa caacgcaaaa cgaaattctg
     1141 cgtcttgcgt ctttgcacct ttaagattag gggcttccca tgagtgcgtt aaataagaaa
     1201 agttttctta cttacctgaa agaaggcggt atttacgtcg ttcttttagt tttgctggca
     1261 attattattt tccaggaccc aacattttta agtctgttga acttaagtaa tattctcacc
     1321 cagtcatcgg tgcgtattat tatcgcgctc ggtgtggcag ggttaattgt cacccagggg
     1381 accgaccttt ctgcaggtcg ccaggtgggg ctggcggcag tagtagcggc gacgttgttg
     1441 cagtccatgg ataacgccaa caaagtgttc ccggaaatgg cgacgatgcc gattgcgctg
     1501 gttattctga ttgtctgtgc cattggtgcg gtgatcggtt tgattaacgg cctgattatc
     1561 gcttatctca acgtgacgcc attcattacc acgctcggca cgatgatcat cgtctatggc
     1621 atcaactcgc tctattacga ctttgtcggg gcgtcgccaa tttctggttt tgacagtggc
     1681 ttctctacct ttgctcaggg ctttgtcgcg ctggggagtt tccgtctctc ttacatcacc
     1741 ttctatgcgt tgattgcggt ggtgtttgtc tgggtgttgt ggaacaaaac ccgcttcggt
     1801 aagaacattt ttgccattgg cggtaacccg gaagcggcga aagtatctgg tgtcaacgtc
     1861 ggcctgaacc tgctgatgat ctacgcgttg tctggtgtgt tctatgcctt cggcgggatg
     1921 ttagaagccg gacgtatcgg ctcagccacc aataacctcg gctttatgta tgagctggat
     1981 gctatcgcgg cgtgcgtggt aggcggcgta tcgttcagcg gcggtgtggg gacggtgatt
     2041 ggcgtggtga ccggggtaat tatttttacc gtcatcaact atggcctgac gtatatcggc
     2101 gtaaacccat actggcagta catcatcaaa ggggcgatta ttatcttcgc cgtagcgctg
     2161 gattcactga aatacgcgcg taagaaatga tgtgaatgtg ggtattgccg gatgcgctgc
     2221 atccggcatt cggtgccaaa tgccggatgt gacgcgggtt ctacaacgtt accgggtgtt
     2281 ctttctcgcc tttcttaaac ttcacttctc cgagatcaat acaacccacc gggcagacgt
     2341 gaccacaaag cagacaaccc acgcatttct cggtattaca atgcggtgta cgggttttct
     2401 cgctccattc catcgcctgg tgaccgccgt cgtaacagga aatataacag cgtccacagc
     2461 caacacattt atcgagattg atatggggat agacaatata actgcggtct aaatcttccg
     2521 ccgggacaat attgttattc gccagaccta ccatctcctg caacgaatca aatccctgat
     2581 cggtgagata atgcgacaac ccgctcgcca tatcttccac tatccgatac ccgtactgca
     2641 taatgccggt ggtcacctgc aacgtcgctg caccgagcaa taaaaactcc gccgcatctt
     2701 cccaggtttc aataccgccg ataccgctga tcgggaaatc gcgcagctct ggatgggtac
     2761 gcatttgctg aatgaagcgc agggcgatcg gttttaccgc tttaccggaa tatccggaaa
     2821 tactcgattt accgttaacg atcggcatac cgattttctg attaagatcg atattggtga
     2881 tggatttaac ggtgttaatc gccgcaatgc catctgcgcc gccgcgcttc gccgccagcg
     2941 ccacttcgca catatcaccg atattcggcg tcatcttcgc cagcattggc agcgtggaac
     3001 cacgttttac tgcccgacaa tatttttcta ccagctccgg gctttgcccg acatcgctac
     3061 ccatcgcatg agaagtcatt tgcggacagg agaagttaca ctcgatcata tccgcgccag
     3121 cttcttgcac cagacgcgcc agctcttccc attgctgctc attttcgccc atgattgaag
     3181 cgatcaatac tttgtccggg taatcttctt tcagccgacg cagggcggcc agattctctt
     3241 ccaacggatg ttcagcaatc tgctccatat ttttgaagcc gataaaaccg gtatcttctt
     3301 tcaccagatg atcaaaacgc ggcgagactt cgttggcgat aaaaaagccg atcgttttaa
     3361 acaccacgcc gccccagcca gtgtcgtagg ctttggcaca catctcatag cagttgccta
     3421 ccggcgaaga agagaggcag aacgggttgg gaaacttcac gccgcaaaaa gtaatcgaaa
     3481 gatctttcgt taacatgagc aagctccctc taaatagtga tgaatcgcct cggcggcttc
     3541 tttcccggtt ttcacggcat agaccacggt tttgtccccc tcaacaatat cgccagcagc
     3601 aaagacttgc ggatcgcggg tctggtaatt ttgtgtattg atggtgttac gctgcggctc
     3661 taactccgca aaggcatcca gtctggcatg ctgaccgacg gcgagaataa ttttatctgc
     3721 cgccatcgtc agttcgcccg gtaaccgtac atgcttaaac gtgactttat tgccttccac
     3781 ggctactggc gtgaatccat caatgatcga aacgcccagc tcccgggcgc tggcaaactc
     3841 tttttcgctt gccggaaact catctaactc ttcacgcgct acgcaagtta ctgcctgaca
     3901 gccgagaact ttcagcgtgc tggctacgtc catcgcgaca tcaccgccgc cgataattaa
     3961 tgcgctttgt ggaatgctga tatcgccttg cgcctgtcgt gcacgttgca ggaagtcgac
     4021 ggcaatctca acgtcgctat gctcaaacag tggtagcccc gaaccgctcg ataaccccac
     4081 ggtgaccagt accgcgcggt tttccgcttt cagctgctcc agggtgagtg tgttaccgat
     4141 ttcgttattg catttaatgg tcacgcccat tttttctata cgggcgattt ctgcatccag
     4201 caccgattgc ggtaaccgga attgcggaat gccgttacgc aaccagccac cgggttgtgc
     4261 ttctttctca tagatcgtga cgtcataacc ctggtttgtc agcgtcacac tggcctgcaa
     4321 tccggcagga cccgcgccaa taatggcgac tttgccgagc gttttagtac cgggctgata
     4381 aatttccatc ccggtttgtt gttcaaaatc agtaacaaaa cgctgtaagc gaccgatatc
     4441 aatgggagta tcaacaccag cacgtgtaca accgctttgg cataatttct ccgtcgggca
     4501 cactctggca caaacggcac cgagggcatt attttcacga attgtctcgg cagcaccttt
     4561 aaaattacga aaataaattg agcggataaa tttccctggg tcagtttgcg ccggacaagc
     4621 ctgactacag ggggcgtcat ggcataataa acagcgagag gcttctttaa tcgccagtaa
     4681 aggcgtaaaa gccggagtga gttcatccag ataattttgt tgcggcatgt gatcttcctt
     4741 aatgctatga gtaattaagg cgaacgctaa ataaatattg cgtgctgata ttaaggctat
     4801 gcgcgtgggg aaatttaaac tgtgaaagga ttcacaaata gctgcggttc agaatgttat
     4861 atccgcagct tattgagtga agtaataatt attctcaata attaacagaa aagagaaaat
     4921 attaacgata gaaaataaga taaacaatca tgccgataga taaaattccg aagccgatat
     4981 atgccaccat ataagcgaag aagagtccgg gattttttgc tcgccagaca tacaccggtt
     5041 ccggtgcatt tttaacccgt ttatcaatcg cgcctgcgcg ccagcctttc catgcttcgc
     5101 ggaaacaaaa tatcgggata aggaaaaaaa cggcaacgac aaaaaactgc tgtacatcca
     5161 taactacttt ccttgtttct tttgtaattc aagtaactgt tcgggtgtga cggcgggata
     5221 cccctgcgca tcttccggca cctggtgcat agccggaata gggaccagcg gccctaaaaa
     5281 acgcggttca cgtttaaaaa tataaaggtc agccagcgca ccgaaacggg cggcaaattc
     5341 gcgaatacgt actgacagca tatctttcgg tgacggtacg gcataacact gagcctgaat
     5401 ccccatatgc agcgcaataa ataatgcccg ctcacagtgg aaacgttggg tgataataat
     5461 gaaatcatta gtatcgaaaa ctttgcgtgt acgcacgatg gaatccagcg tacgaaagcc
     5521 tgcgtaatcg agaacaatat ctgatgggtc gacaccagct gcgattaaat ctttgcgcat
     5581 ggtcatcggc tcattataac tttgcaatgc gttatcgccg ctcagtaata gataatttac
     5641 cttaccgctg ttataggcat taatcgctcc ttgaatgcgg tagcgataat actgattaat
     5701 tacgccagta cgataatatt ttgctgtgcc gagcaccacg ccgacctggc ggtaggggag
     5761 atcctgcaat tcgtcgtaga tataaggcgc ggttttccag ctcatccagc gatcgaggcc
     5821 gagcacagtc aacagcagca agccgatcag gactaacagg ctgaggaaca cgcgctttaa
     5881 catgcaggag actcgatcta gggaaaattt ccatcaggct actttacccg ctggttttgc
     5941 gcaagaaacg gcaaactgtt tgccgggatt ttcgacacca ggagagggct ggcaagccgg
     6001 acaaggcgtt cacgccgcat ccggcaccag gcttaagcga gaagtacgcg gtcgatactg
     6061 tgacagccga gagctttcag cgttgcggag gtggcatccc actgaatcaa cggcgcatcg
     6121 gctttttctg ccagaaccgc gcgctggata tccgggtaat cataaccctt gagattcaat
     6181 agaattaacg ctccctgcaa cggtggcaga gtcgggttga atgcggcgtt ttcagcgtaa
     6241 ctgccgctga aaatacgacc gtctttacat tccagcgcga caccgcttgg cgacttactg
     6301 taaggcatgt gcgaacggtt tgccgccgca atcgctgcct gagaaagcgc atcaccagtc
     6361 agcgcatagc cgtgatcctg ctcgtccatc agcagtgttt taatctccag atctttcggc
     6421 ccaaaggcat ccggtagata gtcacgcagc gcgtgtgcct cgcgtcccgg aagatgaata
     6481 cgcagatcca gaccgctgtt cagttcattc ataaactgac ggcagtgacc acaaggcgtg
     6541 tagttaacgg tgatggctgc aagcgctttt tcaccactca gccaggcgtg gctgatcgcg
     6601 ctttgttcgg catgaacggt ttgctgcatt gtcgcaccaa taaattccat attggcaccg
     6661 aaataccagg ttccgctcac accgcgcgca attgcgccaa cattaaaatt cgacaatggc
     6721 gtacgcgcac aggccgccgc cagcggaagt agtgcgaatg ccagcgcgtc ttcgtccagg
     6781 cccgttgcgc tcttcagcga tgagacttgc tccccggtca acaaagcggg gaagtacttg
     6841 tctgccagaa taggttccag tgcagattgc aagttatccg caagttgggc aaaagcggtt
     6901 tgaaaacgtg gatgcatggc gttgcctcat aatcatgtaa tgggataaca gtttacgaac
     6961 ccgttatggc tttatatgtg atctgaatct cattaattat gcaacgcgtg aaacttacat
     7021 caaaaatgcg cgacgcatcg caaattttag cccattactg ccagaataat cgggaaaagg
     7081 aacggtgcga tcagcgaagt aataatcccg cataacacca gcgccagcga actaaatgca
     7141 ccttcctgat aatccagctc agcacagcgt gccgtaccga gggcgtgcga ggctgtcccc
     7201 atcgccagac cgcgcgctgc tttggtgcga atacgcatcg cattaagtaa cgtatgacca
     7261 aacactgcgc ccagaatacc gacgaaaatc acgcaaactg cgctgattgc cggaataccg
     7321 ccaatactgc cgccaaccgc cattgcaata ggcgtggtga ctgatttcgg caggattgac
     7381 gcggcgattt ccggtgaagc gcccatcaac aatgccacgg aagtccccgt caccatcgca
     7441 accacgctgc cgataaaaca gatggtgatg atcgatttcc agcgcgcgcg gatctggtgt
     7501 agctgctcat ataaaggata ggccagcgca acgaccgccg gttgcagcag atcgtttagc
     7561 acttcgctac ctttaaagta gctgtcgtaa gagatgccgg tcagcattaa aaaaggaatg
     7621 atcaccacca ttgctaccag caacgggtta agcaagggaa acttataccg tgccgccagt
     7681 ttgcgggcgg caaaaaagac aatcaatgtc agcggtaatg accaccagat attcgccatc
     7741 attcttctga tcctttctga cctacgactt tacgttcacc gtgcaccagt tgcgaactcc
     7801 agctcaccac cagaaaaacc accagcgtac tgactgcaca ggaaaccact accgggccaa
     7861 actgtgcgcg gagcaaatca aaatattgca tgacgcctac gccaatcggc acaaacaata
     7921 gcgccatata gcgaatcagt acgtagcacc ccggattgac ccattttgcc ggaagaattt
     7981 gcaaggccag caggacaaac aggatcagca tcccgatgat gctgcccgga atggttaccg
     8041 gtagcaggga agcaatgaaa atgcctgcat acaggcaggc ataaatcagg acaaaagcac
     8101 gtaaatattg ccagataatg ttcagtgtct tactcataat caacacccca gatgagatgg
     8161 attcatcata caattaattc gtaaaacgtg ctaccgatca cattaagaaa tcagcgctta
     8221 ttttttcgct cccgggcatt acgcaatgcg cagcgaactc aggcatacat caagcataga
     8281 gagggattgc agtttgcagc aatccctctt tgcagacctt taatccgttg actataggcg
     8341 ctaacatagc gcctcatttt tttgcgggtg atgatatgcg tgtgttactg gcaccgatgg
     8401 agggtgtact cgactctctg gtgcgtgaat tgctgaccga agttaacgac tacgatctgt
     8461 gcatcaccga gtttgtccgc gtggtggatc aactgctgcc ggtaaaagtc tttcatcgca
     8521 tttgccctga gctacaaaac gccagccgga caccatccgg tacgctggtg cgcgtgcagt
     8581 tgttaggtca gttcccacaa tggctggcag agaatgccgc ccgtgcggtc gagttaggtt
     8641 cctggggcgt ggatctcaat tgcggctgcc cgtcgaaaac ggttaacggt agcggcggtg
     8701 gggcgacgtt actcaaagat cctgaactca tctatcaggg tgcaaaagcg atgcgcgaag
     8761 ctgtaccggc gcatttgccc gtcagcgtaa aagtgcgtct gggctgggac agcggtgaga
     8821 agaaatttga aatcgccgat gcggttcaac aggctggtgc tacagagctg gtggtgcatg
     8881 ggcggacgaa agagcagggt taccgcgcgg agcatattga ctggcaggcg attggcgaga
     8941 ttcgccagcg gctgaatatt ccggtgattg ccaacggtga aatctggaac tggcagagtg
     9001 cgcaacaatg catgacgatc agcggatgcg acgcagtgat gattggtcgc ggggcgctca
     9061 atattcccaa cctgagccgg gtggtaaaat ataacgaacc gcgaatgccg tggccggagg
     9121 tagttgcttt gctgcaaaaa tatacccgtc tggaaaagca gggcgatacc gggttatatc
     9181 acgtagcgcg gattaaacag tggttgagtt atttgcgtaa agaatacgat gaagcaacag
     9241 aattatttca gcatgttcgg atgttgaata attcccctga tattgcaagg gctattcagg
     9301 caattgatat cgagaaactc taagaaacat agtgatgttt tttgacaatg tgttagctct
     9361 ttatcaatag gccgcacggt tgtttggtgg cgtcaaaatc gccgaggcgt tccctgaagg
     9421 ccggggcagc ccacatggat gtgggctgag ggcgcgtttt acagggataa tgagatggac
     9481 gctccagatt ttacggtatc aaagtaccag actggatcgt tgcaatacgt cctg
//
LOCUS       DAMZFT010000177         9528 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.177, whole genome shotgun sequence.
ACCESSION   DAMZFT010000177 DAMZFT010000000
VERSION     DAMZFT010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9528)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9528)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9528
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.177"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..2687
                     /gene="lacZ"
                     /locus_tag="PN977_004533"
     CDS             <1..2687
                     /gene="lacZ"
                     /locus_tag="PN977_004533"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HDI6044482.1"
                     /translation="DECWLQKGQTRIIFDGVNSAFHLWCNGRWVGYGQDSRLPSEFDL
                     SAFLRAGKNRLAVMVLRWSDGSYQEDQDMWRMSGIFRDVSLLHKPTTQISDFHVATRF
                     NDDFSRAVLEAEVQMCGELRDELRVTVSLWQGETQVASGTTPFGGEIIDERGGYADRV
                     TLRLNVENPALWSAEIPNLYRAVVELHTADGTLIEAEACDVGFREVRIENGLLLLNGK
                     PVLIRGVNRHEHHPLHGQVMDEQTMVQDILLMKQNNFNAVRCSHYPNHPLWYTLCDRY
                     GLYVVDEANIETHGMVPMNRLTDDPRWLPAMSERVTRMVQRDRNHPSVIIWSLGNESG
                     HGANHDALYRWIKSVDPSRPVQYEGGGADTTATDIICPMYARVDEDQPFPAVPKWSIK
                     KWLSLPGELRPLILCEYAHAMGNSLGGFAKYWQAFRQYPRLQGGFVWDWVDQSLIKYD
                     ENGNPWSAYGGDFGDTPNDRQFCMNGLVFADRTPHPALTEAKHQQQFFQFRLSGRTIE
                     VTSEYLFRHSDNELLHWSVALDGKPLASGEMPLDVAPQDKQLIELPELPQPESTGQLW
                     LTVHVVQPNATAWSEAGHISAWQQWRLAENLSVALPSAPHAIPQLTTSEMDFCIELGN
                     KRWQFNRQSGFLSQMWIGDEKQLLTPLRDQFIRAPLDNDIGVSEATRIDPNAWVERWK
                     AAGHYQAEVALLQCTADILADAVLITTAHAWQHQGKTLFISRKTYRIDGSGQMAITVD
                     VEVASDTPHPARIGLTCQLAQVAERVNWLGLGPQENYPDRLTAACFDRWDLPLSDMYT
                     PYVFPSENGLRCGTRELNYGPHQWRGDFQFNISRYSQQQLMETSHRHLLHAEEGTWLN
                     IDGFHMGIGGDDSWSPSVSAEFQLSAGRYHYQLVWCQK"
     gene            2739..3992
                     /gene="lacY"
                     /locus_tag="PN977_004534"
     CDS             2739..3992
                     /gene="lacY"
                     /locus_tag="PN977_004534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactose permease"
                     /protein_id="HDI6044483.1"
                     /translation="MYYLKNTNFWMFGLFFFFYFFIMGAYFPFFPIWLHDINHISKSD
                     TGIIFAAISLFSLLFQPLFGLLSDKLGLRKYLLWIITGMLVMFAPFFIFIFGPLLQYN
                     ILVGSIVGGIYLGFCFNAGAPAVEAFIEKVSRRSNFEFGRARMFGCVGWALCASIVGI
                     MFTINNQFVFWLGSGCALILAVLLFFAKTDAPSSATVANAVGANHSAFSLKLALELFR
                     QPKLWFLSLYVIGVSCTYDVFDQQFANFFTSFFATGEQGTRVFGYVTTMGELLNASIM
                     FFAPLIINRIGGKNALLLAGTIMSVRIIGSSFATSALEVVILKTLHMFEVPFLLVGCF
                     KYITSQFEVRFSATIYLVCFCFFKQLAMIFMSVLAGNMYESIGFQGAYLVLGLVALGF
                     TLISVFTLSGPGPLSLLRRQVNEVA"
     gene            4058..4669
                     /gene="lacA"
                     /locus_tag="PN977_004535"
     CDS             4058..4669
                     /gene="lacA"
                     /locus_tag="PN977_004535"
                     /EC_number="2.3.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactoside O-acetyltransferase"
                     /protein_id="HDI6044484.1"
                     /translation="MNMPMTERIKAGKLFTDMCEGLPEKRLRGKTLMYEFNHSHPSEV
                     EKRESLIKEMFATVGENAWIEPPVYFSYGSNIHIGRNFYANFNLTIVDDYTVTIGDNV
                     LIAPNVTLSVTGHPVHHELRKNGEMYSFPITIGNNVWIGSHVVINPGVTIGDNSVIGA
                     GSVVIKDIPPNVVAAGVPCRVIREINERDKHYYYKDYKVEPSV"
     gene            complement(4757..5938)
                     /gene="cynX"
                     /locus_tag="PN977_004536"
     CDS             complement(4757..5938)
                     /gene="cynX"
                     /locus_tag="PN977_004536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308421.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyanate transporter CynX"
                     /protein_id="HDI6044485.1"
                     /translation="MRNHQKTFTMLLVLVLIGLNMRPLLTSVGPLLPQLRQASGMSFS
                     VAALLTALPVVTMGGLALAGSWLHQHVSERRSVAISLLLIAVGALMRELYPQSALLLS
                     SALLGGVGIGIIQAVMPSVIKRRFQHRTPLVMGLWSAALMGGGGLGAAITPWLVQHSE
                     TWYQTLAWWALPAVVALFAWWWQSAREVASSHKTTTTPVRVVFTPRAWTLGVYFGLIN
                     GGYASLIAWLPAFYIEIGASAQYSGSLLALMTLGQAAGALLMPAMARHQDRRKLLMLA
                     LALQLVGFCGFIWLPLQLPVFWAMVCGLGLGGAFPLCLLLALDHSAQPAIAGKLVAFM
                     QGIGFIIAGLAPWFSGVLRSISGNYLMDWAFHVLCVVGLMIITLRFAPARFPQLWVKE
                     A"
     gene            complement(5944..6414)
                     /gene="cynS"
                     /locus_tag="PN977_004537"
     CDS             complement(5944..6414)
                     /gene="cynS"
                     /locus_tag="PN977_004537"
                     /EC_number="4.2.1.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyanase"
                     /protein_id="HDI6044486.1"
                     /translation="MIQSQINRNIRLDLADAILLSKAKKDLSFAEIADGTGLAEAFVT
                     AALLGQQALPADAARLVGAKLDLDEDAILLLQMIPLRGCIDDRIPTDPTMYRFYEMLQ
                     VYGTTLKALVHEKFGDGIISAINFKLDVKKVADPEGGERAVITLDGKYLPTKPF"
     gene            complement(6445..7104)
                     /gene="cynT"
                     /locus_tag="PN977_004538"
     CDS             complement(6445..7104)
                     /gene="cynT"
                     /locus_tag="PN977_004538"
                     /EC_number="4.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase CynT"
                     /protein_id="HDI6044487.1"
                     /translation="MKEIIDGFLKFQREAFPKREALFKQLATQQSPRTLFISCSDSRL
                     VPELVTQREPGDLFVIRNAGNIVPSYGPEPGGVSASVEYAVAALRVSDIVICGHSNCG
                     AMTAIASCQCMDHMPAVSHWLRYADSARVVNEARPHPDLPSKAAAMVRENVIAQLANL
                     QTHPSVRLALEEGRIALHGWVYDIASGSIAAFDGATRQFVPLAANPRVCAIPLRQPTA
                     A"
     gene            7214..8113
                     /gene="cynR"
                     /locus_tag="PN977_004539"
     CDS             7214..8113
                     /gene="cynR"
                     /locus_tag="PN977_004539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414872.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator CynR"
                     /protein_id="HDI6044488.1"
                     /translation="MLSRHINYFLAVAEHGSFTRAASALHVSQPALSQQIRQLEESLG
                     VPLFDRSGRTIRLTDAGEVWRQYASRALQELGAGKRAIHDVADLTRGSLRIAVTPTFT
                     SYFIGPLMADFYARYPGITLQLQEMSQEKIEDLLCRDELDVGIAFAPAHSPELEAIPL
                     LTESLALVVAQHHPLAACEQVALSRLHDEKLVLLSAEFATREQIDHYCEKAGLHPQVV
                     IEANSISAVLELIRRTSLSTLLPAAIATQHDGLKAISLAPPLLERTAVLLRRKNSWQT
                     AAAKAFLHMALDKCAVVGGNESR"
     gene            complement(8246..>9528)
                     /gene="codA"
                     /locus_tag="PN977_004540"
     CDS             complement(8246..>9528)
                     /gene="codA"
                     /locus_tag="PN977_004540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cytosine/isoguanine deaminase"
                     /protein_id="HDI6044489.1"
                     /translation="SNNALQTIINARLPGKEGLWQIYLQDGKISAIDAQSGVMPITEN
                     SLDAEQGLVIPPFVEPHIHLDTTQTAGQPNWNQSGTLFEGIERWAERKALLTHDDVKQ
                     RAWQTLKWQIANGIQHVRTHVDVSDATLTALKAMLEVKQEVAPWIDLQIVAFPQEGIL
                     SYPNGEALLEEALRLGADVVGAIPHFEFTREYGVESLHKTFALAQKYDRLIDVHCDEI
                     DDEQSRFVETVAALAHREGMGARVTASHTTAMHSYNGAYTSRLFRLLKMSGINFVANP
                     LVNIHLQGRFDTYPKRRGITRVKEMLESGINVCFGHDDVFDPWYPLGTANMLQVLHMG
                     LHVCQLMGYGQINDGLNLITHHSARTLNLQDYGIAAGKSANLIILPAENGFDALRRQV
                     PVRYSVRGGKVIASTKPAQTTVYLEQPEAIDYKR"
BASE COUNT         2220 a         2525 c         2552 g         2231 t
ORIGIN      
        1 ttgatgaatg ctggctacag aaaggccaga cgcgaattat ttttgatggt gttaactcgg
       61 cgtttcatct gtggtgcaac gggcgctggg tcggttacgg ccaggacagc cgtttgccgt
      121 ctgaatttga cctgagcgca tttttacgcg ccggaaaaaa ccgcctcgcg gtgatggtgc
      181 tgcgctggag tgacggcagt tatcaggaag atcaggatat gtggcggatg agcggcattt
      241 tccgtgacgt ctcgttgctg cacaaaccga ccacacaaat cagcgatttc catgttgcca
      301 ctcgctttaa tgatgatttc agccgcgcgg tactggaggc agaagttcag atgtgcggcg
      361 aactgcgcga tgagctgcgg gtgacggttt ctttgtggca gggtgaaacg caggtcgcca
      421 gcggcaccac gcctttcggc ggtgaaatta tcgatgagcg tggtggttat gccgatcgcg
      481 tcacgctacg tctgaacgtc gaaaacccgg cgctgtggag cgccgaaatc ccgaatctct
      541 atcgtgcggt ggttgaactg cacaccgccg acggcacgct gattgaagca gaagcctgcg
      601 atgtcggttt ccgcgaagtg cggattgaaa atggtctcct gctgctgaac ggcaagccgg
      661 tgctgattcg cggcgttaac cgtcacgagc atcatcctct gcatggtcag gtcatggatg
      721 agcagacgat ggtgcaggat atcctgctga tgaagcagaa caactttaac gccgtgcgct
      781 gttcgcatta tccgaatcat ccgctgtggt acacgttgtg cgaccgctac ggcctgtatg
      841 tggtggatga agccaatatt gaaacccacg gcatggtgcc aatgaatcgt ctgaccgatg
      901 atccgcgctg gctaccagcg atgagcgaac gcgtaacgcg aatggtgcag cgcgatcgta
      961 atcacccgag tgtgatcatc tggtcgctgg ggaatgaatc aggccacggc gctaatcacg
     1021 acgcgctgta tcgctggatc aaatctgtcg atccttcccg cccggtgcag tatgaaggcg
     1081 gcggagccga caccacggcc accgatatta tttgcccgat gtacgcgcgc gtggatgaag
     1141 accagccctt cccggctgtg ccgaaatggt ccatcaaaaa atggctttcg ctgcctggag
     1201 aactgcgccc gctgatcctt tgcgaatatg cccacgcgat gggtaacagt cttggcggct
     1261 tcgctaaata ctggcaggca tttcgtcagt acccccgttt acagggcggc ttcgtctggg
     1321 actgggtgga tcagtcgctg attaaatatg atgaaaacgg caacccgtgg tcggcttacg
     1381 gcggtgattt tggcgatacg ccgaacgatc gccagttctg catgaacggt ctggtatttg
     1441 ccgaccgcac gccgcatccg gcgctgacgg aagcaaaaca ccagcagcag tttttccagt
     1501 tccgtttatc cgggcgaacc atcgaagtga ccagcgaata cctgttccgt catagcgata
     1561 acgagctcct gcactggtcg gtggcactgg atggcaagcc gctggcaagc ggtgaaatgc
     1621 ctctggatgt tgctccacaa gataaacagt tgattgaact gcctgaacta ccgcagccgg
     1681 aaagcaccgg acaactctgg cttacggtac acgtagtgca accgaacgcg accgcatggt
     1741 cagaagccgg acacattagc gcctggcagc agtggcgtct ggcggaaaac ctcagtgtgg
     1801 cactcccctc cgcgccccac gccatcccgc aactgaccac cagcgaaatg gatttttgca
     1861 tcgagctggg taataagcgt tggcaattta accgccagtc aggctttctt tcacagatgt
     1921 ggattggcga tgaaaaacaa ctgctgacgc cgctgcgcga tcagttcatc cgcgcaccgc
     1981 tggataacga cattggcgta agtgaagcga cccgcattga ccctaacgcc tgggtcgaac
     2041 gctggaaggc ggcgggccat taccaggccg aagtggcgtt gttgcagtgc acggcagata
     2101 tacttgccga cgcggtgctg attacgaccg ctcacgcgtg gcagcatcag gggaaaacct
     2161 tatttatcag ccggaaaacc taccggattg atggtagtgg tcaaatggcg attaccgttg
     2221 atgttgaagt ggcgagcgat acaccgcatc cggcgcggat tggcctgacc tgccagctgg
     2281 cgcaggtcgc agagcgggta aactggctcg gattagggcc gcaagaaaac tatcccgacc
     2341 gccttactgc ggcctgtttt gaccgctggg atctgccatt gtcagacatg tatacccctt
     2401 acgtcttccc gagcgaaaac ggtctgcgct gcgggacgcg cgaattgaat tatggcccac
     2461 accagtggcg cggcgacttc cagttcaata tcagtcgcta cagccaacaa caactgatgg
     2521 aaaccagcca tcgccatctg ctgcacgcgg aagaaggcac atggctgaat atcgacggtt
     2581 tccatatggg gattggtggc gacgactcct ggagcccgtc agtgtcggcg gaattccagc
     2641 tgagcgccgg tcgctaccat taccagttgg tctggtgtca aaaataataa taaccgggca
     2701 ggccatgtct gcccgtattt cgcgtaagga aatccattat gtactattta aaaaacacaa
     2761 acttttggat gttcggttta ttctttttct tttacttttt tatcatggga gcctacttcc
     2821 catttttccc gatttggcta catgacatca accatatcag caaaagtgat acgggtatta
     2881 tttttgctgc tatttctctg ttctcgctat tattccaacc gctgtttggt ctgctttctg
     2941 acaaactcgg gctgcgcaaa tacctgctgt ggattattac cggcatgtta gtgatgtttg
     3001 cgccgttctt tatttttatc ttcgggccac tgttacaata caacatttta gtaggatcga
     3061 ttgttggtgg tatttatctt ggcttttgtt ttaacgccgg tgcgccagca gtagaggcat
     3121 ttatcgagaa agtcagccgt cgcagtaatt tcgaatttgg tcgcgcgcgg atgtttggct
     3181 gtgttggctg ggcgctgtgt gcctcgattg tcggtatcat gttcaccatc aataatcagt
     3241 ttgttttctg gctgggttct ggctgtgcac tcatcctcgc cgttttactc tttttcgcca
     3301 aaacggatgc accctcttcc gccacggttg ccaatgcggt aggtgccaac cattcggcat
     3361 ttagccttaa gctggcgctg gaactgttca gacagccaaa actgtggttt ttgtcactgt
     3421 atgttattgg cgtttcctgc acctacgatg tttttgacca acagtttgct aatttcttta
     3481 cttcgttctt tgctaccggt gaacagggta cgcgggtatt tggctacgta acgacaatgg
     3541 gcgaattact taacgcctcg attatgttct ttgcgccact gatcattaat cgcatcggtg
     3601 ggaaaaacgc cctactgctg gctggcacta ttatgtctgt acgtattatt ggctcatcgt
     3661 tcgccacctc agcgctggaa gtggttattc tgaaaacgct gcatatgttt gaagtaccgt
     3721 tcctgctggt gggctgcttt aaatatatta ccagccagtt tgaagtgcgt ttttcagcga
     3781 cgatttatct ggtctgtttc tgcttcttta agcaactggc gatgattttt atgtctgtac
     3841 tggcgggcaa tatgtatgaa agcatcggtt tccagggcgc ttatctggtg ctgggtctgg
     3901 tggcgttggg cttcacctta atttccgtgt tcacgcttag cggccccggt ccgctttctc
     3961 tactgcgtcg tcaggtgaat gaagtcgctt aagcaatcaa tgtcggatgc ggcgcgagcg
     4021 ccttatccga ccaacatatc aggacggaat gatcgcaatg aacatgccaa tgaccgaaag
     4081 aataaaagca ggcaagctat ttaccgatat gtgcgaaggc ttaccggaaa aaagacttcg
     4141 tgggaaaacg ttaatgtatg agtttaatca ctcgcatcca tcagaagttg aaaaaagaga
     4201 aagcctgatt aaagaaatgt ttgccacggt aggggaaaac gcctggatag aaccacccgt
     4261 ctatttctct tacggttcca acatccatat aggccgcaat ttttatgcaa atttcaattt
     4321 aaccattgta gacgactaca cggtaacgat cggtgataac gtattgattg cacccaatgt
     4381 tactctttcc gttacgggac accctgtaca ccatgaattg agaaaaaacg gtgagatgta
     4441 ctcttttccg ataacgattg gcaataacgt ctggatcgga agtcatgtgg tcattaatcc
     4501 aggcgtcacc atcggggata attctgttat tggcgcgggt agtgtcgtca taaaagacat
     4561 tccaccaaac gtcgtggctg ctggcgttcc ttgccgggtt attcgcgaaa taaacgagcg
     4621 ggataagcac tattattaca aagattataa agttgaacct tcagtttaaa taagcactga
     4681 aataaatgtc ggatgcgcca ttgggcacat ccgacaataa ttcactcttc cattatctgg
     4741 cgaaacaagc atcgcatcat gcctctttga cccacagctg cggaaaacgt gctggtgcaa
     4801 aacgcagggt tatgatcatc agcccaacga cgcacagcac atgaaacgcc cagtccatca
     4861 ggtaattgcc gctgatacta cgcagcacac cagaaaacca cggggcaagc ccggcgatga
     4921 taaaaccgat cccctgcata aacgccacca gtttgccagc aatagcgggt tgcgcagagt
     4981 gatcgagcgc cagcagcaaa cagagcggaa atgcgccgcc cagacctaaa ccacacacca
     5041 tcgcccagaa gaccggcaat tgcagcggca gccagataaa gccgcagaaa cccaccagtt
     5101 gtaacgccag cgccagcatc aaaagtttgc gccgatcctg atggcgagcc atagcaggca
     5161 tcagcaatgc ccccgcagct tgcccaagcg tcatcaacgc cagcaaggaa ccgctgtact
     5221 gcgcgctggc accaatctca atatagaaag cgggtaacca ggcaatcagg ctggcgtaac
     5281 caccgttaat cagaccgaag taaacaccca gcgtccacgc gcggggagtg aataccacgc
     5341 gaaccggagt ggttgttgtc ttgtgagaag aggcgacctc gcgggcgctt tgccaccacc
     5401 aggcaaagag cgcaacaacg gcaggcagcg cccaccaggc gagtgtttga taccaggttt
     5461 cgctatgttg aactaaccag ggcgtaatgg cggcaccaag tccaccgccg cccatcagag
     5521 ccgcggacca cagccccatc accagtggcg tacggtgctg aaaccgccgc ttaattaccg
     5581 aaggcatcac tgcctgaatg atgccgatcc ccactccacc aagcagtgcg ctgctaagca
     5641 acagcgcact ttgggggtaa agctcacgca tcaatgcacc gacggcaatc agtaacaaac
     5701 tgatggcgac gctgcgacgt tcgctgacat gctgatgaag ccagcttccg gccagcgcca
     5761 gcccgcccat ggtaaccacc ggcagagcgg tcaacagggc agccacgcta aagctcattc
     5821 cgcttgcctg gcgcaattgc ggtagcagtg gcccgacgga ggtgagcagt ggtcgcatat
     5881 taagaccaat cagcaccaga accagcagca tcgtgaacgt cttttgatgg ttacgcatgg
     5941 ctgtcagaac ggtttggtcg gcagatattt accatctaag gtgatgaccg cacgttcgcc
     6001 accttccggg tccgccactt tcttaacgtc gagtttgaag ttaatcgcgc tgataatgcc
     6061 atcgccaaac ttctcatgaa ccaacgcttt cagggttgtg ccgtacacct gcaacatttc
     6121 atagaaacga tacatcgttg ggtcggttgg aatacggtca tcaatgcagc cacgcagtgg
     6181 aatcatctgc aacagtagaa tggcgtcttc gtcgagatcc agcttcgccc cgaccaggcg
     6241 ggcggcgtcg gcaggaagcg cctgctgccc cagcaaagcc gcggttacaa aggcttctgc
     6301 cagaccggtg ccgtcggcaa tctcggcaaa tgagagatct tttttagctt tgctgagcaa
     6361 aatggcatcg gcaagatcaa gacgaatatt gcggttaatt tgcgactgaa tcatggtgga
     6421 tctcctgatg gtttaaaaat aaggttacgc tgcggtcggt tggcgtagcg gtatggcaca
     6481 aacgcgagga ttagcggcca gtggcacaaa ctggcgggtt gcgccgtcaa aagctgcgat
     6541 gctgccgctt gcaatgtcgt agacccagcc gtgcagggcg atccgcccct cttcgagcgc
     6601 caggcgcacc gatggatgag tttgcaaatt agccaactga gcaatgacgt tttcacgtac
     6661 catcgccgca gcttttgacg gtaaatcggg atgcgggcgc gcctcattaa cgacgcgggc
     6721 tgaatcggca taacgcagcc agtgggagac ggcaggcata tggtccatgc actgacagct
     6781 ggcaatggcg gtcatcgcgc cacagttgga atgaccgcaa atcacaatgt cagatacccg
     6841 aagcgcagcg acggcgtact ccaccgaagc agaaacgcca ccgggttccg gcccgtagga
     6901 agggacgata ttgcccgcgt tgcgaataac gaacagatcg ccaggctcac gttgcgtcac
     6961 cagctcaggg accagacggc tgtcggagca ggagataaaa agtgtgcgcg ggctttgctg
     7021 tgtcgccagc tgtttaaaca aggcttcccg cttcggaaat gcctcgcgct ggaatttaag
     7081 gaatccatca ataatctctt tcaccgttaa cctctgtctg tctctggaat gagaggcaga
     7141 ctactcgccg attgtcataa ggtaaaagtc tcatttatga tgagttctat tggatttact
     7201 tataggttgg cgaatgctct ctcgacatat caattatttt cttgccgtgg ctgaacatgg
     7261 cagctttacc cgtgccgcca gtgcgttgca cgtctcccag cctgcgcttt cccagcagat
     7321 tcgccagtta gaggagagtt taggcgtgcc gctgtttgac cgtagcgggc gaacgattcg
     7381 tctcactgat gcgggagaag tctggcgaca gtacgccagc cgggcgttac aggaactggg
     7441 ggcgggtaaa cgggcgattc atgatgttgc cgatctgacg cgaggatcgc tgcgtatcgc
     7501 cgtcactccc acctttacaa gctactttat cggcccttta atggcggatt tctatgcgcg
     7561 ctaccccggc atcacgctcc agctacagga aatgtcgcag gagaaaatcg aagatctgct
     7621 ttgtcgcgac gagctggacg ttgggattgc cttcgcgcct gcgcattcgc cggagctgga
     7681 ggcaattcct ttactgacag aaagtttagc gttagtcgtg gcgcaacatc atccgctggc
     7741 tgcctgtgaa caggtggcgt tgagtcgctt gcatgatgaa aaactggtcc tgctcagcgc
     7801 ggaatttgcc accagagagc aaattgacca ctactgcgag aaagcggggc tacatccaca
     7861 ggtggtcatt gaggcgaact caattagcgc ggttctggag ctgattcgcc gcacttccct
     7921 ttccacattg ttgccagcag cgattgccac acaacatgac gggcttaaag cgatttctct
     7981 tgccccaccg ttactggaga gaacggcggt tttgttgcgg cggaaaaata gctggcagac
     8041 agccgccgcg aaggcatttt tacacatggc gttggataaa tgcgcggttg ttggcggaaa
     8101 tgaatcgcgg taaccactga cacaattgcc tgatgcgcta cgcttatcag gcctacgagt
     8161 tcagtgctct acgtaggccg gataaggcgt tcacgccgca tccggcataa actacgctcg
     8221 ctttgtcaac acacagccca gccgatcacc gtttgtaatc gatggcttcc ggctgctcca
     8281 gatacacggt ggtttgtgcc ggtttcgtgc tggcaatcac cttaccacca cgtaccgaat
     8341 agcgtaccgg aacctgacgg cgcagcgcgt caaacccatt ttcagccggt aggataatca
     8401 ggttagcgct ttttccggcg gcaatgccgt aatcctgcaa attcaacgtc ctggcactgt
     8461 ggtgggtgat taaattcagg ccatcgttaa tctgcccgta gcccatcagc tggcaaacat
     8521 gcagccccat atgcagcact tgcagcatat tcgccgttcc cagcggatac cacggatcga
     8581 agacatcatc gtgaccaaag cagacgttaa tgccggactc cagcatctct ttaacgcgcg
     8641 tgatgccgcg acgttttgga tacgtatcga aacgcccttg cagatgaata ttgaccagcg
     8701 ggttggcgac aaagttaata ccggacattt tcagcaagcg gaacaggcgc gaggtatacg
     8761 ccccgttata ggagtgcatt gccgtagtgt ggctggcggt gactcgcgcg cccatgcctt
     8821 cacggtgcgc cagggcggca acggtttcaa caaagcgcga ctgttcgtca tcaatctcat
     8881 cacagtgaac atcgatgaga cggtcgtatt tttgcgccag ggcgaaggtt ttatgcagcg
     8941 actccacgcc atattcacgg gtaaattcaa aatgcggaat cgcccccact acgtctgccc
     9001 ctaagcgtaa cgcctcttcc agcaacgctt caccgttggg atacgacaaa atcccttcct
     9061 gcgggaaggc gacgatttgc agatcaatcc acggcgcaac ttcctgtttc acttccagca
     9121 tcgctttcaa cgcagtcagc gttgcgtcgg agacatcgac atgggtacgc acatgctgaa
     9181 tgccgttggc aatctgccat ttcagcgttt gccaggcgcg ttgtttcaca tcgtcatggg
     9241 ttaataacgc tttgcgctcg gcccagcgtt caatgccttc aaacagcgtg ccggactgat
     9301 tccagttcgg ttgtccggcg gtttgcgtgg tgtccaggtg aatatgcggc tccacaaacg
     9361 gcggtataac taaaccttgt tcggcatcca ggctgttttc agttatgggc atcacgccgg
     9421 attgcgcatc aatggcgctg atttttccgt cctgcagata aatctgccac agcccctctt
     9481 tgccaggtaa ccgggcgtta ataattgttt gtaaagcgtt attcgaca
//
LOCUS       DAMZFT010000178         9496 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.178, whole genome shotgun sequence.
ACCESSION   DAMZFT010000178 DAMZFT010000000
VERSION     DAMZFT010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9496)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9496)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9496
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.178"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            191..931
                     /locus_tag="PN977_004541"
     CDS             191..931
                     /locus_tag="PN977_004541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001696707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6044490.1"
                     /translation="MKSKYKKLKDELIKIAKACAPTPEDILVYMGRARRFASFLKESN
                     IQIKSINSIKLRHIELYFQQRYRTGVRSKILREELDTIKHILTDCGKRNMMKNERLTY
                     AALNIADVRPIVICTYCGNKAQLRKGALMPFSSTPTTENKYYWICSPCNAWVGCHKNS
                     GRPLGTPAKENLRILRAQVRKLFDSYQQKTNISRNEANRWLSRKLNCRIHECHIGYFN
                     ESMCNRASEILITEINKFAKNTYPPDSF"
     gene            complement(935..1852)
                     /locus_tag="PN977_004542"
     CDS             complement(935..1852)
                     /locus_tag="PN977_004542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001696705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase domain-containing protein"
                     /protein_id="HDI6044491.1"
                     /translation="MSRKSSRSKVEVFRKSFISLARDCGGSYATTADRMRIARYFLNY
                     LQVNGIKLRQTGSIKTRHISGYLLSRKEQGISHRTIQNERAVMRAILEKDGRLKIADP
                     SNPLLSNEALGLRDTCRDGKKIPLSQDEFNKAFSKVEKNNPGVAAVMQLSYVLGLRTK
                     EAVQSCKSVNSWLRELDSGHDSLLVVFGTKGGRPRDTTIINRDAVKHALIYAKNIMDK
                     QNGKLIDRPNIKQAINVYRYHVRKAGLSGVKAPHSMRYHFSQEARQFYRKSGYGDKEI
                     YARVSMDLGHGDGRGRYVKQVYFKGADES"
     gene            3002..3655
                     /locus_tag="PN977_004543"
     CDS             3002..3655
                     /locus_tag="PN977_004543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6044492.1"
                     /translation="MDFAVIFFWSFACVVIFLCLKSSINNQEKMQSLLFIFLLLTGGY
                     LSSHIFNTGSGKWLFITIAITFLLNTALIFLFIFTKAYFFSQHVNKMREKAKQTNSLD
                     FINCLIKLHKKYPVYVLYAPSENTVEICYNIFNVNPVIGKKLYLKTLSNRHIRFTVKN
                     IILLPALNDDFICTLESFYNNSDETKDIIDNYIRKIQGNQQLPWLINNAVPTDTKEK"
     gene            3751..4068
                     /locus_tag="PN977_004544"
     CDS             3751..4068
                     /locus_tag="PN977_004544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001696703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6044493.1"
                     /translation="MNSTRKEKYSAFCRLLNETLKYELRLILPPGHQAVIPLLESPRG
                     EISRDTIEKMRDILTPDVTHRIKESINAWTEDELSYLDCTVDVEYVKEQKRKLFAMLD
                     CEQ"
     gene            complement(4845..5108)
                     /locus_tag="PN977_004545"
     CDS             complement(4845..5108)
                     /locus_tag="PN977_004545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HDI6044494.1"
                     /translation="MKPNIHPEYRTVVFHDTSIDEYYKIGSTIKTERVIELDGVTYPY
                     VTIDVSSKSHPFYTGKLRTVASEGNVARFNQRFGRFVDTKKGA"
     gene            complement(5333..5905)
                     /locus_tag="PN977_004546"
     CDS             complement(5333..5905)
                     /locus_tag="PN977_004546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000703300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6044495.1"
                     /translation="MKISKLVVVATVCSTLSACSVEEMAAVNKKISDGAFAVTKALNG
                     GTGYADNGMPILNKQLPPEQDKRENVIDVPVDVDTAAGRLKRYYKFYSNDEVNAIRNN
                     GTTDGKWQASAIIDNGHIWDAMPGSYYKMGGNWRKGSFDDHLTIEVEKNGKGSRVYIT
                     YASASQAHLQASSLEPLFKRVKDVAEGKVR"
     gene            6203..6448
                     /locus_tag="PN977_004547"
     CDS             6203..6448
                     /locus_tag="PN977_004547"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6044496.1"
                     /translation="MNNETEKAMWSCKTSDTPWTFSSLLKKAVCAVLSNAGSDSTCNE
                     KNKNDIELVKIRSYGDSEGWIESKGGPINIVNSYDRY"
     gene            complement(6497..8005)
                     /locus_tag="PN977_004548"
     CDS             complement(6497..8005)
                     /locus_tag="PN977_004548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006858964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraG N-terminal
                     domain-containing protein"
                     /protein_id="HDI6044497.1"
                     /translation="MTTNNYLEHFLTLLGWLVNNGLWDLLTGTGLFALPLAFRVIGIW
                     LKVREEGADEGNKGMLSLPRIEHALYGAFFVMVACCVPLVQVTLDTLKFDRSRAQTCG
                     VWTPKAPGDTGYSGVISSLGDRTAAAPVWWVVVHKLSKGVTQAAVASIPCRPDIRQLR
                     FEVQRTFIANRALADELQDFTNDCYSLALYQWKQRDQGMTKDRKLLSDISWIGSSTFM
                     QGDYGTLQSKMPRAAFPWNSARDSGRPDTGRGGYPTCREWWSDSSVGLKKRVTEEVDE
                     GLWLRISAAMKMLGNAQANYQEEIIKRLVSPANLTLSQGGEVYRGYGGNADMTAANAV
                     TRAAGTAGSAVASLAAFPAFDAMRQALPMVQSILLMAVYIMLPLILAFAAYEFSTLIT
                     LTFVVFALNFLTFWWELARWLDSWMLTALYSSDTHSRVNMMGLQNTSDDIIMNFVMGT
                     MFIVLPTVWLGALTWAGISVGSIGAAIGKGVSDAQQAGKQAGNIANLLSRKK"
     gene            complement(8002..8361)
                     /locus_tag="PN977_004549"
     CDS             complement(8002..8361)
                     /locus_tag="PN977_004549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001251932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6044498.1"
                     /translation="MAMTQGTKAGLVLKGVKVAGMWLSGLIAMMILALLVADTGMRHT
                     QEVEALRLWLHETRFGWLGWRVCLYGALAWGCRKIWRAPGFRPEYRRPFARICLVSAL
                     FALLCEYSIFFGPGAEL"
     gene            complement(8366..>9496)
                     /locus_tag="PN977_004550"
     CDS             complement(8366..>9496)
                     /locus_tag="PN977_004550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000977109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrating conjugative element protein"
                     /protein_id="HDI6044499.1"
                     /translation="LNGVTEGFKNLMGDVIQGATGAVASLPAMVIQRANPGLYDMITN
                     GVLQANVAFDKAQLNCQNMAKRMMDFSDRSKWTQAAMLEEYKKVVNSGDGDAVKGDEA
                     GRQATGKEGKKWIGGQQRGGAGQPAIRPVHDLTAAGFNMMNGLPVTSTASVSAGNCNG
                     SACEMFRNAEEAAKAVVKVLGDRTMRTCTDAKACTSGDNEQQQGTVAGTGFAPVLEEA
                     TRVNTEQLVRLVNGQEKPTAENLAKLKTGSLAVSAGVINALRRDPDNMSLTSRLAGEL
                     AMAHTVETALVMRRMLLTGMSEPYAAAEDAAIQEGDRRIASLDREIMALKNEMELKRD
                     LARNSVLTIIERDNERVGSNPMIQPADNTDSRVRALETPDGE"
BASE COUNT         2610 a         2539 c         1949 g         2398 t
ORIGIN      
        1 accaccggga gattggttgg tcgccgaagc gacaagttag cgtttaaatt gtaagcacaa
       61 actgcataaa acttcaagtt gttattgtcg tggagcggct gagaagctac aactgaatga
      121 caataaatac ttaatttgat atttttttac tcagtaacac tcttaatgaa ccttaaagag
      181 agatatcagg atgaaatcga aatacaaaaa attaaaagat gaactaatca aaatagctaa
      241 agcatgcgcc ccgacaccag aagacatact ggtatatatg gggagagcca gaagatttgc
      301 ttcatttctt aaagaatcca atatacagat aaaatcaata aatagtataa aactacgtca
      361 tattgagctt tactttcaac agcgttatcg aactggcgtc cgttcaaaaa tactccgaga
      421 agaattagac acaataaagc atattctcac tgactgtgga aaacgaaaca tgatgaaaaa
      481 tgaacgtctg acttatgcag cactgaatat tgcagatgtt cgtccaattg taatctgcac
      541 ttactgtggt aataaggcac aactcagaaa aggtgcactt atgccatttt catcgacacc
      601 cactacagaa aacaaatatt actggatttg cagcccatgc aatgcatggg ttggatgtca
      661 taaaaattca gggagaccat taggtactcc agctaaagaa aatttgagaa tactcagagc
      721 acaagtgcgt aaactttttg acagctatca gcaaaaaaca aatatatccc gtaacgaggc
      781 aaacagatgg ctatccagaa aactaaattg ccgcatccat gaatgccata taggatactt
      841 taatgagagc atgtgtaatc gtgcatcaga aattcttatt acagaaatta ataagttcgc
      901 gaaaaacaca tatcctccgg attcatttta aatatcagct ctcatcagcg cctttaaagt
      961 agacctgttt tacataacgc cctcttccgt caccatgacc aaggtccatg gaaacccttg
     1021 cgtaaatttc tttatctcca tatccacttt ttcgataaaa ctgccttgct tcctgcgaaa
     1081 aatgataacg catactatgt ggtgccttca ctccagataa accagccttt cttacatggt
     1141 aacgatatac gtttatagcc tgtttaatat tggggcgatc gataagttta ccattctgtt
     1201 tatccataat gttttttgca taaatgagcg catgtttcac tgcatcacga ttaatgatcg
     1261 ttgtatctct gggccttcca cctttcgtcc caaaaacaac gagaagagaa tcatgccctg
     1321 aatcaagttc acgtagccac gagttaactg atttacaaga ctgaacagct tcttttgtcc
     1381 tcagtccaag aacataagaa agttgcatta ctgccgcaac accgggatta tttttttcaa
     1441 ccttactgaa ggctttatta aactcatcct gtgagagtgg tatcttttta ccatcacggc
     1501 atgtatccct gagtccaaga gcttcattgc tcaataaagg attggacgga tccgctattt
     1561 taaggcgacc atctttctcc agaattgcgc gcataactgc ccgttcattc tgaattgtgc
     1621 ggtgagagat cccctgctct ttcctgctga gaagataacc agaaatatgc ctcgtcttaa
     1681 tactgcccgt ctgacgaagt ttgataccgt ttacttgcaa ataattcaaa aagtatctgg
     1741 caattcgcat tctgtcagcg gttgttgcgt agctgccacc acaatcacgg gcaagtgaga
     1801 tgaatgactt gcgaaagact tcaactttgg aacgtgacga tttacgggac ataacgtcaa
     1861 tccccctgtt aaaaaagatg cagaaaacca tccgttctga aagttcaact gcatgagatg
     1921 cccttccgat aaacagacca gatcgttgat attattagtt tttcgtcatc aaggacggct
     1981 ctgtaacgcg gttgctcgcc ttcctggctc gtttgatgtc gatgtagcag agcttgcaca
     2041 tgttgagttt ttacaggagt tcgctacctg tgcaccaggg cgatttttta ctccccgtca
     2101 tcttacgatg acgccttccg acttcagaac gatttttcat atttattcct tttttcaaca
     2161 agttaataca cttacctctc atgtattatg aagtttaaaa agacattccg gcgtggccgt
     2221 ccgtgaaaac ggacgcgccg gaaaggcatg gacacgttac tgaaagacgc tgtcagaaag
     2281 acaaaacggt catcagaacg ctggtcacga gcgacgattt tctggcaggc cttacgtgat
     2341 gctgctttca gggcagattg ttgtggcgtc acttggtgtt ccctccggtc gcacgcaagt
     2401 gacgccgcac agcttcagac tccacgtaca cacgcaagca gccaaatttc aaatggcctt
     2461 atgccgtgcg cacagtcaca agaagctcat gcagccagac agaaaatctt taagacgcag
     2521 atatataagg cattttcgcc actctgcgag aggaaaactc gtaagtttca tgacatacaa
     2581 aaccgtacgg gtaatccgaa ggtaaaaata acgtacctca gtcaccagct actggtgaca
     2641 tccgtttcca gaggctaaac ggatgttgcc gccatatagg catcctcttc caggctcatg
     2701 ggatattgca gtcacccgaa ggcgctcctc tcagactgcc ggaacattgg ctggtcatcc
     2761 tgagatgaca gtgtggtcgc gtaagcctcc ctctcagacc accgggagat tggttggtcg
     2821 ccgaagcgac agttaaagcg ggtacagaat aacaaaagaa aaaagctatc gtcaacagac
     2881 tgattttatg gcgcatggga aataatgtat tacacaaata aaccaacata ttgatatatt
     2941 taccgcatcg cgaatatcgt gatagcatca acaaatgaac tgtcagacaa aaggtgataa
     3001 tatggatttt gctgttatat ttttctggag ctttgcatgt gttgttatat ttctgtgtct
     3061 gaaatcatca ataaacaacc aggaaaaaat gcagtcatta ttatttatat ttttgttgct
     3121 gacaggtggg tatctttcat cccatatctt taatacaggt tcaggtaaat ggttatttat
     3181 aacaattgcc atcacatttc tgttaaacac tgcgcttatt tttcttttta tatttacaaa
     3241 agcatatttc ttttcgcaac acgtaaataa gatgcgagaa aaagcaaaac aaacaaattc
     3301 cttagacttt ataaattgcc tgataaaact tcataaaaaa tatccggtat acgtattata
     3361 cgcaccttca gaaaacacag tggaaatctg ttacaatatt ttcaatgtaa atcctgttat
     3421 tggaaagaaa ctttatttaa aaacactgag taatcgtcat atccggttca cagtaaaaaa
     3481 cataatcctg ctacctgcat taaatgatga ttttatatgc acacttgaat ctttttataa
     3541 taatagtgat gaaacaaaag atattattga taactacatc cgaaaaatac agggtaatca
     3601 acaattgccg tggctaataa acaatgcagt acccactgac acaaaagaaa agtgagtcca
     3661 tgatattaaa tccggataaa aacaacttgt acatcttttg caaaacgctt taatcttaca
     3721 ccatgcacag taatgagaag gaataaaacg atgaacagta cccggaaaga gaaatacagt
     3781 gctttctgtc gtttactcaa cgaaacactt aaatatgaat tacgtcttat actccctccg
     3841 ggacatcagg cagtaattcc tctgcttgaa tctccacgag gagaaataag ccgggatacc
     3901 attgagaaaa tgcgcgacat actcacaccg gatgtgacac accgaataaa agaatccatc
     3961 aatgcctgga cagaagatga gttaagttat ctggattgta ctgttgatgt tgagtatgtg
     4021 aaggaacaga aacgaaaatt atttgcaatg ctggactgcg aacaataaac cacaaaaaag
     4081 gccagcataa tagctggcca ggtcttacag agcaaaacaa aataacccgt aatatcacag
     4141 gggataatac cgcctctgta tgctaacgcc agccggcaat gacaggcaac aagcgaaact
     4201 gtttttcaga aacagatata gaatggtgat attcatcctt tacccatgta accacacagc
     4261 gtaggtgcat tatgaaagaa ctgtgacgtt ccgcttcctc gtccgctcgc tcactaccgt
     4321 tcggtcgccg gactgcggcg agcggactct ccagaatcac atcctgataa aacagcctgg
     4381 ttcgctccct ctaccccact attcttttga gacacagatc cccctcaggg tcagttctcc
     4441 gacattcacc ttcttcatct cagccagcgc attcctgcag gccctttcgc tggcgaactc
     4501 ctgagaatga acagcaagcg ttggttgtct ggcaacactg gatgacactg aatatggtgt
     4561 tgaaagccag aaaatcagta tccacatact ccccccttgt ttaacgcaaa cataccgcat
     4621 gttcagacat cacccggaca atcgcagcct tacagctccc agcccgacac agcccccttc
     4681 cctccccaga caacagctaa aaaaaatccc cgtaaccggg gatgaaggaa tatggactgt
     4741 gagtttctgt ttaagtgctt ctttcttgtg aaacgtaaaa taaccgacct cagacactca
     4801 tgcaatacct ttccatatca atcgcgcctg tgatcgattt ctgctcatgc gccttttttg
     4861 gtgtcaacaa aacgaccgaa acgctggtta aagcgcgcca cgttaccttc tgaagcaaca
     4921 gttctcagtt tgccagtgta gaaaggatgc gacttagagg aaacatcaat tgttacgtac
     4981 ggataggtga caccatcaag ctcaatcaca cgttcagtct tgatggtcga accaatttta
     5041 tagtactcat cgatactggt atcgtggaac accacagtac ggtattcagg atggatattc
     5101 ggtttcatcg gttttctccc ccttgagtaa aatttttgcg cacgtaaatt acatctctcc
     5161 ccgttcaatt acaagccctg taacttccag cacgatgaac agaaaacaac accagatgtt
     5221 tcacttatgt aaaaatactg gacaacatgg taaaaaaatg attaaaaaaa gtgggccgcc
     5281 acgggcaatc tccctctcct ttagccataa gggcttgccc gtggcgtgct catcaacgaa
     5341 cctttccttc agccacatcc ttcactcgct taaacagagg ctcaagagaa cttgcctgca
     5401 aatgcgcctg agaagctgaa gcataggtga tatagacccg gcttccctta ccgttctttt
     5461 ccacctcaat cgtcaggtga tcatcaaagc tgcctttacg ccagtttcct cccattttgt
     5521 agtaactgcc cggcatcgca tcccagatat gcccattgtc aataatcgca cttgcctgcc
     5581 attttccgtc agttgtgccg ttattgcgga tcgcatttac ctcatcattc gagtaaaact
     5641 tgtaatagcg tttcagtcgg cctgctgcgg tatccacatc aacagggaca tcaataacat
     5701 tttcgcgttt atcctgctcc ggcggcaatt gcttgttaag gatcggcata ccattatccg
     5761 cgtagcccgt tcccccattg agcgctttcg tcacagcaaa tgcaccgtca ctgattttct
     5821 tgttcacagc agccatctct tccacggaac atgcactaag cgtggaacat actgtagcca
     5881 caacaaccag ttttgatatt ttcattcagc ttctcctgat acagggtttg attcagggag
     5941 aattaaggca tagccggacg actaaaaaga gagattattc ttttcgcatt tttaaaaatt
     6001 aatcacacaa tgttcagcag gactgaggct gaagttcctg gagctattgt gacatgcagt
     6061 gtcacgtcct ggcccacatc acaggctgta cagcaagaag agtaaaatca aacaattaca
     6121 cctggatttt caagcattga acatttatca tacaacacgt atgatagtac caggatggtt
     6181 atccacttac aggaggcacg cgatgaacaa tgaaacagaa aaagcaatgt ggagttgcaa
     6241 aacctctgat accccatgga ctttcagctc tctgctgaaa aaagctgttt gcgcggtttt
     6301 gtctaacgct ggatcagact ctacttgcaa tgaaaaaaac aagaacgata ttgaactcgt
     6361 aaaaatccgg tcatatggag atagcgaggg ctggattgaa tcaaaaggtg gtcccatcaa
     6421 catcgttaat tcatacgatc gatattaata ttcagcagga gaagcaacta ttattctcct
     6481 cctgctgaaa gcttgtttat tttttcctac ttaataaatt ggcaatattt cctgcctgtt
     6541 ttcctgcttg ttgtgcgtca gatacacctt tgccaatagc tgctccaata cttccaacac
     6601 taatccctgc ccatgttaac gctccgagcc atactgtcgg caatacaata aacatcgtgc
     6661 ccatcacaaa attcatgatg atatcgtccg acgtattctg cagccccatc atgttcactc
     6721 gggaatgcgt gtccgaactg tacaacgccg tcagcatcca gctgtcaagc caccgcgcca
     6781 gctcccacca gaacgtcagg aaattcagtg caaacaccac aaacgtcagt gtgatcagcg
     6841 tgctgaattc atacgccgca aacgccagaa tcaacgggag catgatatac accgccatca
     6901 gcagaatact ctgcaccatc ggcagcgcct gccgcatcgc atcaaacgcc ggaaacgctg
     6961 ccagactagc aacggcagaa cccgccgtgc ctgctgcacg cgtgaccgca ttcgccgccg
     7021 tcatatctgc attaccgcca tagccacggt acacctcacc cccctgtgac agggtcaggt
     7081 tcgccggact caccagacgt ttgatgattt cctcctggta gttcgcctgc gcgttcccca
     7141 gcattttcat tgccgccgaa atacgtaacc acagtccttc atcaacttcc tctgtcaccc
     7201 gcttcttcag cccgacacta ctgtcactcc accattcacg gcatgtggga taaccccccc
     7261 gtcccgtatc cggacggccg ctgtcacggg cgctattcca cgggaatgcc gcacgtggca
     7321 ttttcgactg cagtgtcccg tagtcaccct gcatgaacgt actgctgcca atccagctga
     7381 tatccgaaag cagcttcctg tccttcgtca tcccctggtc acgctgcttc cactgataca
     7441 gcgccagcga gtaacagtcg ttggtgaagt cctgcagctc gtcggccagc gcccggttcg
     7501 caatgaatgt ccgctgcacc tcaaaacgca gttgccgtat atccggccgg cagggaatgg
     7561 acgccacagc agcctgcgtc acacctttgg agagtttatg caccaccacc caccacaccg
     7621 gggcggccgc tgtcctgtca cccaggctgc tgatcacccc gctgtacccg gtatcaccgg
     7681 gtgctttcgg tgtccacaca ccgcaggtct gggcacggct gcgatcaaat ttcagcgtgt
     7741 ccagcgtcac ctgtaccagc ggcacacagc aggccaccat cacaaaaaat gcaccataca
     7801 gggcatgctc aatgcggggc agtgagagca tccctttatt tccctcatcc gccccctctt
     7861 cccgcacttt cagccagatc ccgataaccc tgaaggccag tggcagggca aacagccccg
     7921 tccccgtcag cagatcccac aggccgttat tcaccagcca gcccagcagg gtcagaaagt
     7981 gttccagata gttattcgtg gtcacagttc agcccccggt ccgaaaaaaa tgctgtactc
     8041 gcaaagtaac gcaaacagag cactgaccag acaaatccgc gcaaagggac ggcggtattc
     8101 cggtctgaac cccggcgcac gccagatttt ccggcatccc catgccagcg caccgtagag
     8161 gcacacccgc caccccagcc atccgaaacg tgtctcgtgg agccagagcc gtaatgcttc
     8221 cacttcctgc gtatgccgca tgcccgtatc agccaccagg agggccagaa tcatcatggc
     8281 aataaggccc gacagccaca tgccggccac cttaacgccc ttcaggacca gccccgcttt
     8341 tgtcccctga gtcatcgcca tacgcttact ccccgtccgg agtttccagt gcccggacac
     8401 gactgtcagt attgtctgca ggctggatca tcgggttgct gcccacgcgc tcattgtcgc
     8461 gttcaatgat ggtcagcacg ctgttacgtg ccagatcgcg tttcagctcc atctcgttct
     8521 tcagcgccat gatttccctg tccagtgaag cgatacgccg gtcgccttcc tgaatcgcgg
     8581 catcttctgc ggccgcatac ggctcagaca tcccggtgag cagcattctg cgcatcacca
     8641 gagccgtttc cacggtatgc gccatcgcca gttctcccgc cagccgcgat gtcagggaca
     8701 tattgtccgg gtcacgacgc agggcattga tcacccctgc agagacagcc agactgccgg
     8761 ttttcagttt tgccagattc tccgctgtgg gtttctcctg accattcacc agacggacca
     8821 gttgttccgt gttcacccgg gtcgcctctt ccagcaccgg cgcaaacccc gttcctgcaa
     8881 cggttccctg ctgctgttca ttatccccgc tggtacaggc cttcgcatcc gtacaggtgc
     8941 gcatggtacg atcacccagc accttcacca ccgccttcgc ggcctcttct gcattccgga
     9001 acatctcaca ggcgctgccg ttacagttgc cggcactgac gctggccgta ctggtcacgg
     9061 gcagaccgtt catcatgtta aacccggccg ctgtcagatc atgcaccggc cggatggcgg
     9121 gctgacctgc cccgccacgc tgctgtccgc caatccattt cttaccctcc ttcccggtgg
     9181 cctgacgccc ggcctcatcc cctttcaccg catcgccgtc accgctgttc accaccttct
     9241 tgtactcttc cagcatcgca gcctgcgtcc atttgctgcg atcagaaaag tccatcatcc
     9301 gttttgccat gttctggcag ttcagttgcg ccttgtcgaa agccacattc gcctgcagca
     9361 caccgttggt gatcatgtcg tacaggccgg gattggcacg ctgtatcacc atcgccggca
     9421 gactggcaac ggcgccggtc gccccctgaa tcacatcccc catcagattt ttaaaacctt
     9481 cggtcacgcc gttaag
//
LOCUS       DAMZFT010000179         9407 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.179, whole genome shotgun sequence.
ACCESSION   DAMZFT010000179 DAMZFT010000000
VERSION     DAMZFT010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9407)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9407)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9407
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.179"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..681
                     /gene="selB"
                     /locus_tag="PN977_004551"
     CDS             <1..681
                     /gene="selB"
                     /locus_tag="PN977_004551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HDI6044500.1"
                     /translation="LNGEGMRELLQQPGYIQAGYSLLNAPVAARWQRKILDTLATYHE
                     QHRDEPGPGRERLRRMALPMEDEALVLLLIEKMRESGDILSHHGWLHLPDHKAGFSEE
                     QQAIWQKAEPLFGDEPWWVRDLAKETGTDEQAMRLTLRQAAQQGIITAIVKDRYYRND
                     RIVEFANMIRDLDQECGSTCAADFRDRLGVGRKLAIQILEYFDRIGFTRRRGNDHLLR
                     DALLFPEK"
     gene            872..2023
                     /gene="yiaY"
                     /locus_tag="PN977_004552"
     CDS             872..2023
                     /gene="yiaY"
                     /locus_tag="PN977_004552"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine dehydrogenase"
                     /protein_id="HDI6044501.1"
                     /translation="MAASTFFIPSVNVIGADSLNDAMNMMADYGFTRTLIVTDNMLTK
                     LGMAGDVQKALEERNIFSVIYDGTQPNPTTENVAAGLKLLKENNCDSVISLGGGSPHD
                     CAKGIALVAANGGDIRDYEGVDRSAKPQLPMIAINTTAGTASEMTRFCIITDEARHIK
                     MAIVDKHVTPLLSVNDSSLMVGMPKSLTAATGMDALTHAIEAYVSIAATPITDACALK
                     AVTMIAENLPLAVEDGSNAKAREAMAYAQFLAGMAFNNASLGYVHAMAHQLGGFYNLP
                     HGVCNAVLLPHVQVFNSKVAAARLRDCAAAMGVNVTGKNDAEGAEACINAIRELAKKV
                     DIPAGLRDLNVKEEDFAVLATNALKDACGFTNPIQATHEEIVAIYRAAM"
     gene            2153..3448
                     /locus_tag="PN977_004553"
     CDS             2153..3448
                     /locus_tag="PN977_004553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HDI6044502.1"
                     /translation="MAIKSPPGLIPLSHLSGEELLAHLRFNRVTDEKGRYLPFDELQY
                     RIKKGENVDVAWTLTRLARNAAIQRINYCNEAGEQAGFNITPVIAEACELVDKRATAL
                     ALKDQTERLRGAGAELSQLRLEEPITSSQLEGANTTTLVARKMLETGRSPRTEDEHMI
                     AGNARLMAEIPHLLAEPLTPALIRQLHAIGMGGINDAKYRPGEFRETDDVVIADYDGN
                     IVHQPPAAALLPERLEKVCQWLNSHEGYIHPLVRACILHFMLAHEHPFRDGNGRTSRA
                     LFYWYMLKSGYDVFKYISISRLLHAAPVKYAASYQYTESDGMDLTYFLEYQAGVIKRA
                     LQNWQQHIDEITQRSAKLDSVLFSSGVLKRLNPRQVTLLNVMLANPGKEYTVAEISAS
                     LGVSDNTARADLRTIVKEGFAQEKKINDQQAVYFAHYPL"
     gene            3556..5094
                     /gene="aldB"
                     /locus_tag="PN977_004554"
     CDS             3556..5094
                     /gene="aldB"
                     /locus_tag="PN977_004554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001551836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase AldB"
                     /protein_id="HDI6044503.1"
                     /translation="MTNNPPSAQIKPGEYGFPLKLKARYDNFIGGEWVAPADGEYYQN
                     LTPVTGQLLCEVASSGKRDIDLALDAAHKVKDKWAHTSVQDRAAILFKIADRMEQNLE
                     LLATAETWDNGKPIRETSAADVPLAIDHFRYFASCIRAQEGGISEVDSETVAYHFHEP
                     LGVVGQIIPWNFPLLMASWKMAPALAAGNCVVLKPARLTPLSVLLLMEIVGDLLPPGV
                     VNVVNGAGGEIGEYLATSKRIAKVAFTGSTEVGQQIMQYATQNIIPVTLELGGKSPNI
                     FFADVMDEEDAFFDKALEGFALFAFNQGEVCTCPSRALVQESIYERFMERAIRRVESI
                     RSGNPLDSVTQMGAQVSHGQLETILNYIDIGKKEGADVLTGGRRKLLEGELKDGYYLE
                     PTILFGQNNMRVFQEEIFGPVLAVTTFKTMEEALELANDTQYGLGAGVWSRNGNLAYK
                     MGRGIQAGRVWTNCYHAYPAHAAFGGYKQSGIGRETHKMMLEHYQQTKCLLVSYSDKP
                     LGLF"
     gene            complement(5313..5456)
                     /locus_tag="PN977_004555"
     CDS             complement(5313..5456)
                     /locus_tag="PN977_004555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6044504.1"
                     /translation="MNKNRKTSTMQIKRIKPSIIYRLLLIGLGTPMVIYGLVRPLTIE
                     TRD"
     gene            5638..5961
                     /locus_tag="PN977_004556"
     CDS             5638..5961
                     /locus_tag="PN977_004556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HDI6044505.1"
                     /translation="MFLDYFALGVLIFVFLVIFYGIIILHDIPYLIAKKRNHPHADAI
                     HVAGWVSLFTLHVIWPFLWIWATLYRPERGWGMQNHDSSVVQLQQRIAGLEKQLADIK
                     SSSAE"
     gene            5967..7103
                     /locus_tag="PN977_004557"
     CDS             5967..7103
                     /locus_tag="PN977_004557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HDI6044506.1"
                     /translation="MDLLIILTYVAFAWAMFKIFKIPVNKWTIPTAALGGIFIVSGLI
                     LLMNYNHPYTFKAQKAVISIPVVPQVTGVVIEVTDKKNTLIKKGEVLFRLDPTRYQAR
                     VDRLMADIVTAEHKQRALGAELDEMAANTQQAKATRDKFAKEYQRYARGSQAKVNPFS
                     ERDIDVARQNYLAQEASVKSSAAEQKQILSQLDSLVLGEHSQIASLKAQLAEAKYNLE
                     QTIVRAPSDGYVTQVLIRPGTYAASLPLRPVMVFIPDQKRQIVAQFRQNSLLRLAPGD
                     DAEVVFNALPGKVFSGKLAAISPAVPGGAYQSTGTLQTLNTAPGSDGVIATIELDEHT
                     DLSALPDGIYAQVAVYSDHFSHVSVMRKVLLRMTSWVHYLYLDH"
     gene            complement(7100..8074)
                     /locus_tag="PN977_004558"
     CDS             complement(7100..8074)
                     /locus_tag="PN977_004558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDI6044507.1"
                     /translation="MTKLQLKYRELKIISVIAASENISHAATVLGIAQANVSKYLADF
                     ESKVGLKVFDRTTRQLMLTPFGTVLLPYINDMLDRNEQLNNFIADYKHEKRGRVTIYA
                     PTGIITYLSKHVIDKIKDIGDITLSLKTCNLERNAFYEGVEFPDDCDVLISYAQPKDE
                     SLVASFITQYAVTAYASQRYLEKHPISRPDELEHHSCILIDSMMIDDANIWRFNVAGS
                     KEVRDYRVKGNYVCDNTQSALELARNHLGIVFAPDKSVQSDLQDGTLVPCFQHPYEWW
                     LDLVAIFRKREYQPWRVQYVLDEMLREIRHQLAQSQQLRPEQAAESED"
     gene            8198..8938
                     /locus_tag="PN977_004559"
     CDS             8198..8938
                     /locus_tag="PN977_004559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MipA/OmpV family protein"
                     /protein_id="HDI6044508.1"
                     /translation="MLINRNIVALFALPFMASATASELSIGAGAAYNESPYRGYNENT
                     KAIPLISYEGDSFYVRQTTLGFILSQSEKNELSLTASWMPLEFDPADNDDYAMQQLDK
                     RDSTAMAGVAWYHHERWGTVKASAAADVLDNSNGWVGELSVFHKMQIGRLSLTPALGV
                     LYYDENFSDYYYGISESESRRSGLASYSAQDAWVPYVSLTAKYPIGEHVVLMASAGYS
                     ELPEEITDSPMVDRNESFTFVTGVSWRF"
     gene            complement(9068..>9407)
                     /locus_tag="PN977_004560"
     CDS             complement(9068..>9407)
                     /locus_tag="PN977_004560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 127 protein"
                     /protein_id="HDI6044509.1"
                     /translation="HVAGKVAIQRGPLVYCLEQADNGESLHNLWLPADAPFTTFEGKG
                     LFRHKILIQAPGYRYEQSNPEQQPLWHYDYAPAKRQPQTLTFIPWFSWANRGEGEMRI
                     WVNEEKHCHP"
BASE COUNT         2327 a         2219 c         2471 g         2390 t
ORIGIN      
        1 ctcaacggcg aagggatgcg cgaactgctg caacagcctg gctatattca ggctggttat
       61 agcttgttga atgcgccggt tgccgcccgc tggcagcgga aaattctcga cacattagcg
      121 acttatcatg agcaacatcg cgatgaacct ggtcctgggc gcgaacgtct gcgacgtatg
      181 gcgttgccaa tggaagatga agcgctggta ctgttgctga ttgaaaagat gcgcgaaagc
      241 ggcgacatcc tcagtcatca cggctggctg catctgccgg atcacaaagc gggcttcagc
      301 gaagagcagc aggccatctg gcaaaaagca gagccactgt ttggtgacga accgtggtgg
      361 gtgcgagacc tggcaaaaga gacgggaacc gacgagcagg caatgcgcct gactctacgc
      421 caggcggcgc agcaggggat aattaccgcg atcgttaaag atcgttatta ccgtaacgat
      481 cggattgtcg agtttgctaa tatgatccgc gatctcgatc aggagtgtgg ttcaacctgc
      541 gcggcggatt tccgcgatcg cttaggcgta ggccgaaagc tggcaattca gattctggaa
      601 tattttgacc gcattggctt tacgcgtcgt cgtggaaatg atcatttatt acgcgacgca
      661 ttattatttc cggaaaaata aggaaatgat taattcataa taaatatgaa ttaatacaaa
      721 atttcttatt aatgttaaga aggcacgtta tttgatgaat gtaacgtgct ttttattaaa
      781 aatattttaa taaacggtaa tgttgatcat attttcaaat gaaaattctc agtaagctgc
      841 ccgccctttt ttacactttc aggagtgtgt tatggcagct tcaacctttt ttattccttc
      901 cgtgaatgtt attggtgctg attcattgaa tgatgcaatg aatatgatgg cagattatgg
      961 atttacccgt accttaattg tcaccgacaa tatgttaacg aaattgggta tggcgggtga
     1021 tgtgcaaaaa gcactggaag aacgcaatat ttttagcgtt atttatgatg gcacccaacc
     1081 taaccccacc acggaaaacg tcgccgcagg tttgaaatta cttaaagaaa ataattgcga
     1141 tagcgtgatc tccttaggcg gtggttctcc gcacgactgc gcaaaaggta ttgcgctggt
     1201 ggcagccaat ggcggcgata ttcgcgatta cgaaggcgtt gaccgctctg caaaaccgca
     1261 gctgccgatg atcgccatca ataccacggc gggtacggcc tctgaaatga cccgtttctg
     1321 catcatcact gacgaagcgc gtcatatcaa aatggcgatt gttgataaac atgtaactcc
     1381 gttgctttct gtcaatgact catctctgat ggtcggtatg ccgaagtcac tgaccgccgc
     1441 aacgggtatg gatgccttaa cgcacgctat cgaagcgtat gtttctattg ccgccacgcc
     1501 gatcactgac gcttgcgccc tgaaagccgt gaccatgatt gccgaaaacc tgccgttagc
     1561 cgttgaagat ggcagtaatg cgaaagcgcg tgaagcaatg gcttatgccc agttcctcgc
     1621 cggtatggcg ttcaataatg cttctctggg ttatgttcat gcgatggcgc accagctggg
     1681 cggtttctac aacctgccac acggtgtatg taatgctgtt ctgctgccac acgttcaggt
     1741 attcaatagc aaagtcgctg cagcacgtct gcgtgactgt gccgctgcaa tgggcgtgaa
     1801 cgtgactggt aaaaacgacg cggaaggtgc tgaagcctgc attaacgcca tccgtgaact
     1861 ggcgaagaaa gtggatatcc cggcaggcct gcgtgacctg aacgtgaaag aagaagattt
     1921 cgcggtactg gccaccaatg ccctgaaaga tgcgtgtggg tttactaacc cgattcaggc
     1981 aactcacgaa gagattgtgg caatttatcg cgctgcgatg taatcatcat tcccacaatg
     2041 gctggcgagt tgtcagccgc tttttcaact atctcaaaac ttgctttttt tgagatagtt
     2101 tctctttcct gctatatact atctcaatat tgcgcttttt tgagataagc acatggctat
     2161 caaatcacct cccggactta ttcctttatc acacttatcg ggtgaggaac tcctggccca
     2221 cctgcgcttt aaccgggtta ccgatgaaaa aggacgttat ctgccttttg atgagttgca
     2281 gtaccgcatc aaaaaaggag aaaacgtcga tgtggcgtgg acgcttaccc gtctggcgcg
     2341 caacgcggcg atccaacgaa ttaattattg caatgaagcg ggtgaacagg ccggattcaa
     2401 cattaccccg gtgattgcag aagcgtgcga gcttgtcgac aaacgtgcta ccgcgctggc
     2461 gctgaaagat cagactgaac ggttacgcgg ggccggagcc gagttaagcc agttacgcct
     2521 ggaagagccg atcaccagct cccagctgga gggtgcgaat actacaacgc tggtggcgag
     2581 aaaaatgctg gaaacagggc ggtcgccacg cactgaagac gagcatatga ttgcgggaaa
     2641 tgcccggctg atggcggaaa ttccgcactt actcgcagaa ccgctgacac ctgcgcttat
     2701 tcgccagtta catgccattg gtatgggtgg aatcaatgac gcgaaatatc gtccgggaga
     2761 atttcgtgaa acagatgatg ttgtgatcgc cgactatgat ggcaatatag tgcatcaacc
     2821 gcccgctgcg gcattgttac cggaacggct ggaaaaagtt tgccagtggt taaacagcca
     2881 tgaagggtat attcatccgt tggtccgtgc ctgcattctg cattttatgc tggcccatga
     2941 acatccgttt cgggatggca acgggcgcac cagtcgggcg ctgttttact ggtatatgct
     3001 gaaatcgggc tacgatgtct ttaaatatat ttccatcagc cgtttgcttc atgctgcacc
     3061 ggtcaaatat gccgccagct accagtacac tgaatcagat ggtatggatc tgacttattt
     3121 ccttgaatat caggcgggtg tcattaaacg tgctctgcaa aactggcagc agcatatcga
     3181 tgaaattaca caacgaagcg caaaactcga tagtgtgctg tttagctctg gtgtgcttaa
     3241 acgcctgaat ccgcgccagg taacgctcct gaatgtgatg ctcgccaatc cggggaaaga
     3301 gtacacagtt gctgaaatta gcgcgagcct gggcgtctct gataatacgg ctcgagctga
     3361 tttacggaca attgttaaag aagggtttgc gcaagagaag aaaattaatg accagcaagc
     3421 cgtgtacttc gcgcattatc cgctgtaacc cttgcccgta aattcgtgat agccgtcgta
     3481 aagctgttat cgactggcga agatttcgcc agtcacgtct acccttgtta tacctcacac
     3541 cgcaaggaga cgatcatgac caataatccc ccttcagcac agattaagcc cggcgagtat
     3601 ggtttccccc tcaagttaaa agcccgctac gacaacttta ttggcggcga atgggtagcc
     3661 cctgccgacg gcgagtatta ccagaacctg acgccggtga ccggacagct gctgtgcgaa
     3721 gtggcgtctt cgggcaaacg agacatcgat ctggcgctgg atgctgcgca caaagtgaaa
     3781 gataaatggg cgcatacttc ggtgcaggat cgcgcggcga ttctgtttaa gattgccgat
     3841 cggatggagc agaacctcga actgttagcg accgctgaaa cctgggataa cggcaaaccc
     3901 attcgcgaaa ccagtgctgc tgatgtgccg ctggcgattg accattttcg ctattttgcc
     3961 tcgtgtattc gggcgcagga aggcggtatt agtgaagtgg atagcgaaac tgtggcttat
     4021 cacttccacg aaccgttagg cgtggtgggg cagattattc cgtggaactt cccgctgctg
     4081 atggcgagct ggaaaatggc tcccgcgctg gcggcgggca actgtgtggt gctgaaaccc
     4141 gcacgtctta ccccgctctc agtactgctg ctaatggaaa tcgtcggtga tttactgccg
     4201 ccgggcgtgg tgaacgtggt caacggtgcg ggcggagaaa ttggcgaata tctggcgacc
     4261 tcgaaacgca tcgccaaagt ggcgtttacc ggctcaacgg aagtgggcca acaaattatg
     4321 cagtacgcca cgcaaaacat tattccggtg acgctggagc tgggcggcaa atccccaaat
     4381 atcttctttg ctgatgtgat ggatgaagaa gatgcctttt tcgataaagc gctggaaggc
     4441 tttgcactgt ttgcctttaa ccagggcgaa gtttgcacct gtccaagccg tgctttagtg
     4501 caggagtcta tctacgaacg ctttatggaa cgcgccatcc gccgtgtcga aagcattcgc
     4561 agcggtaacc cgctcgacag cgtgacgcaa atgggggcgc aggtttcgca cgggcaattg
     4621 gaaaccatcc tcaactacat tgatattggt aaaaaagagg gcgcagacgt gctcaccggc
     4681 gggcggcgca agctgctgga aggcgaactg aaagacggct actacctcga accgacgatt
     4741 ctgtttggtc agaataatat gcgcgtgttc caggaagaaa tttttggtcc ggtgctggcg
     4801 gtgaccacct tcaaaacgat ggaagaagcg ctggagctgg cgaacgacac gcaatatggc
     4861 ctgggcgcgg gtgtctggag ccgcaacggt aatctggcct ataagatggg gcgcggcatt
     4921 caggccgggc gcgtgtggac caactgttat cacgcttacc cggcacatgc ggcgtttggc
     4981 ggctacaaac agtcgggcat cggacgcgaa acccataaga tgatgcttga gcattaccag
     5041 caaaccaagt gcctgctggt gagctactcg gataaaccgt tggggctgtt ctgatataag
     5101 aagctggtcg cattgggtat tcattgcctg atgcgacgct tacgcgtctt atcatgccta
     5161 cggggacctg accgtaggcc ggataaggcg tacacgccgc atccggcata aacaaagcgc
     5221 acgttgttac aatttacgtt ttaaatctac gtgcgcaata acccagacta cgccaacaga
     5281 agtgcgtttc tggggcgtgt tttgccagga gtttaatctc gcgtttcgat ggtgagcggg
     5341 cgaaccaggc cataaatcac cattggcgtt ccgaggccaa tcagcaataa acggtagata
     5401 attgaaggtt taatacgctt aatctgcatt gtggatgttt tacggttttt attcataaat
     5461 ttggtcaata gttacattat ttcaatatga taagcttcga cctattgaaa agccaggcac
     5521 acacttcaga aagtatataa aaaaccatat gtcgttatat aaataaggaa attgcaccgc
     5581 attatggtat ttcataatat gcgttaattg ttctttttta tattctctga gggaaatatg
     5641 ttcctggatt attttgcact gggagtgctt atttttgtat ttctggtgat cttctatggg
     5701 attattattt tacatgatat tccctacctg attgctaaaa aacgtaatca tccacatgcc
     5761 gacgccattc atgttgctgg ttgggtaagt ctttttacgt tacatgttat ctggccattt
     5821 ttgtggattt gggccacgct ttaccgcccg gagcgtggat ggggaatgca aaaccatgac
     5881 tcctccgttg tgcaactaca acagcgtatt gccgggctgg agaaacagct cgctgacatc
     5941 aaatcctctt ctgccgagta atacttatgg atttactgat tattttaacc tatgtggctt
     6001 tcgcatgggc aatgtttaag atctttaaaa ttcctgtaaa taaatggacc attcccacag
     6061 cggccctggg aggcatattt attgtcagcg gtctaattct gttaatgaac tataaccatc
     6121 cgtatacctt taaagcgcaa aaagcggtta tttctattcc tgttgtccca caggtgacag
     6181 gcgtggtgat cgaagtgacg gacaagaaaa atacgctgat taaaaaaggt gaggtgctat
     6241 ttcgactgga cccgacgcgt tatcaggcgc gggtggatcg gctgatggcg gatatcgtta
     6301 ccgcagaaca taaacagcgg gcgttgggcg cagagttaga tgagatggcg gcgaatactc
     6361 agcaggcaaa ggccacgcgg gataaattcg ctaaagagta tcagcgttac gcacgcggca
     6421 gtcaggcgaa agtaaacccg ttttcagaac gcgatatcga tgtggcgcgg caaaattatc
     6481 tggcgcagga agcctccgtg aagtcatcgg cggcggaaca aaaacagatc ctgagccagc
     6541 tggatagcct ggtgttgggt gaacattctc aaatcgccag cctgaaagcg cagctcgcgg
     6601 aagcaaaata taaccttgag cagacgatag tgcgtgcgcc gagcgatggt tatgtgaccc
     6661 aggtactgat tcgtccgggt acctatgccg cgtcgctgcc gctacgtccg gtgatggtgt
     6721 ttatacccga tcagaaacga caaatcgtgg cgcagttccg tcagaactcc ttgctgcgac
     6781 tggctcctgg cgacgatgcg gaagtggtgt ttaatgctct gccaggtaag gtattcagcg
     6841 gtaagctggc agccattagt ccagccgttc ccggcggagc ttatcagtcg accggcacct
     6901 tacagacgtt aaacacagcg ccgggttcag atggcgttat cgcgaccatt gaactggatg
     6961 agcatactga tttgagcgca ttaccagacg gtatttacgc ccaggtggcg gtctactctg
     7021 atcatttcag ccatgtctcg gtgatgcgca aagtactgtt acgcatgacc agctgggtgc
     7081 attaccttta tctcgatcat taatcttcgc tttccgccgc ttgttcaggc cgcagttgct
     7141 gcgactgagc aagctggtgg cgaatttcac gcagcatttc atccagcaca tactggaccc
     7201 gccagggctg gtattcccgt ttacggaaga tagccaccag atccagccac cattcatacg
     7261 ggtgctgaaa gcagggcacc agcgtgccgt cttgcaggtc gctctgcaca cttttgtctg
     7321 gcgcaaacac aatccccagg tgatttcgtg ccagctccag cgcagattgc gtgttgtcgc
     7381 aaacgtaatt tcccttcacg cgataatccc gcacctcttt actcccggcg acgttgaagc
     7441 gccagatatt cgcgtcgtcg atcatcatcg agtcgatcaa aatacaggag tgatgttcga
     7501 gttcgtcagg acggctaatg ggatgtttct caagatagcg ctggctggca taagcggtta
     7561 cggcatattg ggtaataaaa ctggcaacca gcgattcatc ttttggttgg gcgtagctga
     7621 ttaaaacatc acaatcatcg ggaaattcga cgccttcata aaaggcatta cgctcaagat
     7681 tgcaggtttt tagcgataag gtgatatcac cgatatcttt aattttatcg attacatgtt
     7741 tggataaata ggtaattatg ccggttggtg catagatagt gacccgacca cgtttctcat
     7801 gcttataatc tgcaataaaa ttattaagct gctcgtttct gtccagcatg tcgttgatgt
     7861 acggtaacag cacggtgcca aaaggtgtga gcatcagctg tcgggtggtt cggtcaaaga
     7921 cttttaaacc cacttttgat tcaaaatcag caagatattt gctgacgttg gcctgtgcga
     7981 tgccaagtac agtcgcggca tggctgatat tttcactggc agcgattacc gagataattt
     8041 ttaactcccg gtacttaagt tgtaattttg tcatcgcaac agcccaatta tatataattt
     8101 tatatattac tatataaata tgaatcttgc accggagaaa ttgtaagcat tactatgccg
     8161 actctttttt atcttatttt aaaatggaat accggacatg ttaattaacc gcaatattgt
     8221 ggcgttattt gcgttgcctt ttatggcaag cgcaactgct tctgaattat ccattggtgc
     8281 tggtgcggct tataatgaat cgccttatcg cggttataat gaaaatacaa aggcaattcc
     8341 gctgattagt tatgaaggtg attcttttta tgttcgtcag accacgttag gttttattct
     8401 gtcgcaaagt gaaaaaaatg aacttagcct gaccgcatcc tggatgccgc tggaatttga
     8461 ccctgccgat aatgacgatt atgccatgca acagcttgat aagcgtgata gtacggctat
     8521 ggcgggggtt gcctggtatc accacgagcg ttggggaacc gtgaaagcct ctgcggctgc
     8581 ggacgttctg gataacagca acggctgggt gggtgagcta tcggtattcc acaaaatgca
     8641 gataggtcgc ctgtcgctga cacctgcgct gggcgttctc tattatgacg agaatttcag
     8701 tgactattac tatggcattt cagagagtga gtcccgtcgt agcggtctgg caagttattc
     8761 cgcgcaggat gcctgggtgc cctatgtcag cctgacggca aaatacccga taggagagca
     8821 cgtcgtattg atggcgagcg ccggatacag cgagctgccg gaagagatta ccgacagccc
     8881 gatggttgat cgtaatgaga gttttacctt tgtcaccggc gtgagctggc gtttttaatt
     8941 caccggtgga tgtcggtgcg gctggcctac gttctcgcac cgactcgtgg gcctgataag
     9001 acgcgttaag cgccgcatca ggcaatgtgc accctacggt ggatgcggcg cgagcgcctt
     9061 atccaaccta cggatggcaa tgcttttcct cattcaccca gatccgcatt tcgccttcac
     9121 cccggttagc ccagctaaac cacgggataa atgtcagagt ttgcggctgg cgtttggctg
     9181 gggcatagtc gtaatgccac agcggttgct gctctggatt gctctgttca taccggtaac
     9241 ccggtgcctg gattaagatc ttatggcgga acaatccctt gccttcaaat gtcgtaaatg
     9301 gcgcatcggc gggcagccac aggttatgca gtgactcgcc gttgtcggcc tgttccaggc
     9361 aatacaccag cgggccgcgc tgaatcgcca cttttccggc gacgtga
//
LOCUS       DAMZFT010000180         9369 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.180, whole genome shotgun sequence.
ACCESSION   DAMZFT010000180 DAMZFT010000000
VERSION     DAMZFT010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9369)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9369)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9369
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.180"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(<1..564)
                     /locus_tag="PN977_004561"
     CDS             complement(<1..564)
                     /locus_tag="PN977_004561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001757010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HDI6044510.1"
                     /translation="MIQTRNQYLQFMLVMLAAWGISWGARFVMEQAVLLYGSGKNYLF
                     FSHGTVLMYLLCVFLVYRRWIAPLPVVGQLRNVGVPWLVGAMAVVYVGVFLLGKALAL
                     PAEPFMTKLFADKSIPDVILTLLTIFILAPLNEETLFRGIMLNVFRSRYCWTMWLGAL
                     ITSLLFVAAHSQYQNLLTLAELFLVGLI"
     gene            complement(662..802)
                     /locus_tag="PN977_004562"
     CDS             complement(662..802)
                     /locus_tag="PN977_004562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004031751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6044511.1"
                     /translation="MAESGYGLAGLGMGKVKSINQYRLTPGFGGFTPVSYETTECRLP
                     CR"
     gene            complement(1778..1990)
                     /locus_tag="PN977_004563"
     CDS             complement(1778..1990)
                     /locus_tag="PN977_004563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6044512.1"
                     /translation="MNGFRNSSRNGQVWCWQRAGSRAVILEVSGRRMEAAEAWRRAAG
                     VAPRTDWQQFARKRAAHCHRRCRGRG"
     gene            complement(2121..2483)
                     /locus_tag="PN977_004564"
                     /pseudo
     CDS             complement(2121..2483)
                     /locus_tag="PN977_004564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052671.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fertility inhibition protein FinO"
     gene            complement(2479..5730)
                     /gene="traI"
                     /locus_tag="PN977_004565"
                     /pseudo
     CDS             complement(2479..5730)
                     /gene="traI"
                     /locus_tag="PN977_004565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490592.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative transfer relaxase/helicase TraI"
     gene            complement(5730..7430)
                     /gene="traD"
                     /locus_tag="PN977_004566"
     CDS             complement(5730..7430)
                     /gene="traD"
                     /locus_tag="PN977_004566"
                     /inference="COORDINATES: protein motif:HMM:NF010254.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system coupling
                     protein TraD"
                     /protein_id="HDI6044513.1"
                     /translation="MQWKAIRIGDLPIIRDSEIQNFCLHGTVGAGKSEVIRRLANYAR
                     QRGDMVVIYDRSGEFVKSYYDPSIDKILNPLDARCAAWDLWKECLTQPDFDNTANTLI
                     PMGTKEDPFWQGSGRTIFAEAAYLMRNDPNRSYSKLVDTLLSIKIEKLRTFLRNSPAA
                     NLVEEKIEKTAISIRAVLTNYVKAIRYLQGIEHNGEPFTIRDWMRGVREDQKNGWLFI
                     SSNADTHASLKPVISMWLSIAIRGLLAMGENRNRRVWFFCDELPTLHKLPDLVEILPE
                     ARKFGGCYVFGIQSYAQLEDIYGEKAAATLFDVMNTRAFFRSPSHKIAEFAAGEIGEK
                     EHLKASEQYSYGADPVRDGVSTGKDMERQTLVSYSDIQSLPDLTCYVTLPGPYPAVKL
                     SLKYQARPKVAPEFIPRDINPEMENRLSAVLAAREAEGRQMASLFEPEVASGEGVTQA
                     EQPQQPQQPQQPQQPQQPQQPQQPVSSVINDKKSDAGVSVPAGGIEQELKMKPEEEME
                     QQLPPGISESGEVVDMAAYEAWQQENHPDIQQQMQRREEVNINVHRERGEDVEPGDDF
                     "
     gene            complement(7414..9024)
                     /gene="traG"
                     /locus_tag="PN977_004567"
                     /pseudo
     CDS             complement(7414..9024)
                     /gene="traG"
                     /locus_tag="PN977_004567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001007007.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer mating pair stabilization
                     protein TraG"
     gene            complement(9072..9162)
                     /locus_tag="PN977_004568"
                     /pseudo
     CDS             complement(9072..9162)
                     /locus_tag="PN977_004568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001605962.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraB"
     gene            complement(9158..9369)
                     /locus_tag="PN977_004569"
                     /pseudo
     CDS             complement(9158..9369)
                     /locus_tag="PN977_004569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858331.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methylase"
BASE COUNT         2019 a         2740 c         2371 g         2239 t
ORIGIN      
        1 aatcaacccc accaggaaca gttctgccag tgtcagcagg ttctgatact ggctgtgcgc
       61 ggcgacgaac aacaacgacg ttatcagcgc ccccagccac atcgtccagc agtaccgtga
      121 acggaagacg ttcagcataa tcccccggaa cagcgtttcc tcattcaacg gggcaaggat
      181 aaagatggtc agcaacgtca ggatcacgtc aggtatggac ttatcggcaa aaagtttcgt
      241 cataaatggc tcagcaggca gagccagcgc cttaccgagc agaaatacac cgacatacac
      301 cacggccatc gcaccgacca gccacggtac gccaacgttg cgcagctgac caacgaccgg
      361 tagcggagct atccaacggc ggtataccag gaaaacacac agcaggtaca tcagaacagt
      421 accatgactg aagaacaaat agttttttcc tgatccataa agcagaacgg cctgctccat
      481 gacaaatctg gctccccaac taatgcccca tgcagccagc ataaccagca taaactgcag
      541 atattgatta cgtgtttgaa tcattgcatc gcctgtaaat ttttaacttg tcctattttt
      601 gtcattacca cgtatataca catgtataac aattcagata tcgttaccag gatatgccgc
      661 atcagcggca tggaaggcgg cactctgttg tttcatatga tacaggagta aaaccgccga
      721 agcccggcgt aagccggtac tgattgatag atttcacctt acccatcccc agccctgcca
      781 gaccataccc gctttcagcc atgagagagc ttctgtgcgc ggtcggagtg gtcccgacga
      841 gggtttaccc gaagtcgggg cgtatctccg cgttagcggg ccgtgagggc cgcttacgag
      901 cgtgtactga gaacttccag cgagaagact gacagcgatg aagatgtagt tacaacattc
      961 ataattaaaa gcgactctgt tccggcccga agggccgggg cggggccgct tttcagttat
     1021 gagggagggg ctttgtggtt tcagttctgc gctggttcgg ggtttttctg gaggttggtt
     1081 ttgtgtgttg taactaaagt ggctccggtt ggggcccgcc gtttacggtg ggaggtgcat
     1141 atctgtctgt ccataggaca agcagtgaat aggttttctt tttaaatgaa tgtaattaag
     1201 tagtttaaag gagatataaa caggtgttta aaagatacat tgcaccctgt agggctgacg
     1261 gctggcgctt tatgacatta acgattgtaa ccttatgggg aagtcccttg cagtttaatg
     1321 tggatagccg aaaattattg ttcgtatgct cccgctggtc cagaatggga atttcctctg
     1381 gctgatgttg aactgtcatg gagaattgaa acttgtttaa gactttggca gcctactgtt
     1441 tttaatacac ccaataaggt atgaagatga aactagggaa gttaataccg ccatgaaaag
     1501 ataaataata cccgtggaat atatttcctc atgttactct ccatatcaaa taaatggcac
     1561 ctgtatttca gacatcctga atattacagc acacattgaa caacacctta ttttgtgaaa
     1621 ttaatcacaa aatataaaat ctcgacttaa tacggaaaaa aatctgtgct actcagaact
     1681 gtccggtcat acttcccgac cggatattaa aaaaagcaaa tctcataaac ttctttcagc
     1741 cttctgactc ctggagtaat gaaggctgct tttttcgtta cccccttccc cggcaacgcc
     1801 ggtggcagtg tgcagccctt tttcgggcaa actgctgcca gtctgtccgg ggcgcaacac
     1861 cggcagcccg tcgccatgct tctgccgctt ccatcctgcg tccactgacc tccagaataa
     1921 cggcccggct tccggcacgc tgccagcacc agacctgacc gttccgtgaa ctgtttctga
     1981 atccattcat atttctgtcc ccctttagtt gtcagtatta ttgtgataaa tagctcagtg
     2041 gaatggcttt ctttcgggta cggaagttat ttccttttaa gccttatttc cctgcctccc
     2101 ctccctgagg gaggcttttt ttatttctca tcaagcacgg cctgaagttc tgcctttatc
     2161 cggttctggc ggcggatttt atccagacgc gctgccgcat acgcttcctc ctcctgagaa
     2221 atatgctccg tcacataccc ttccgtatca taccggcagg caccggcttt catggcacac
     2281 agatagcttt ctgaacgggt gatggccttc agcgccctgc gcagtttttt atgcgagagc
     2341 gggatattcc gctgcgccac gtcttccagt aacacgtccc ggataccgca ggccagaagc
     2401 cggggcgtgt caccgtcaaa taatcccggc caccagggct tcagggtgtt cacagcctca
     2461 tccagtgtgg gcaggttcag atatcagccc gtcatccttg ctgataccgg caatccggtg
     2521 atacacacta tccaccaggg caagggcatc cgggttattc tcccaggtgg agagacgacg
     2581 cagttctccg tcacgtatat tcgctgtgtt caggacaggc accatgacct gctctttgcc
     2641 cagctcaccg gccttttccc gtgcatcatg aatcatgctg ttcagtacgc gccggtcctc
     2701 attcaggtgc gtgacaatca gcgtctgctc ccgtgcttcc ggtgtcctgc cggtatagtc
     2761 gcggacaatg gcctcataca gtgtcatggg gatatccggg aaagcctcgc ctttcagcat
     2821 cgccttctgc tgcgcttctg ccagtttcgc ttcctggctg tgactgaact ccgtcacgga
     2881 gtgctccggt gcccatgcgc cctcctgacg gggaacctga gacggtttca cactctcaag
     2941 cccggacagt gccctttcca catcccggtt aatcaggctg tataccgcct cccgcagttc
     3001 cggcgtctga cgcacaatct ccttcatgat gaccacatcg gcagcactgc gcgtctgctg
     3061 cagacggaaa ggctgaccgg gcgcgatggc ctgcagctgg tccgtgtcac cactggccac
     3121 ggcacgaccg ccaccggccg caatcagggc gtatgcccgt gccatatcgg tattgcccac
     3181 cattgagctc tcatcgagca ggaacagcgt gttgctgaaa tccggcgttt ctccgctgcg
     3241 ctggcgggca agtttttccg cagtacgggg ttcatccagc gaggaataca gccggacatc
     3301 acgaccactg cgggccagac cgttcagggt cgcgttgtcc atcgccatct gtgtgacgga
     3361 ggcaaaaacc gtcgcactgt cgctgacgga atgcccgggc gtttccaccc agccgttctc
     3421 cagcttcagt gccgtgaatg gtgaatcagc cacaggcagg gtggccggtt cagcccgccc
     3481 cggcacaaca accgtcatcg catcttcact gacggatgcc acctgcaggc ggtcaccgcc
     3541 ggagacgcgg agtccgggaa ttttccctgt caccctcagt cgctcgccgt ctgccaccgg
     3601 cattttttcc ggccggaaca gcgaccagct gctgtccagg gaggaaatac gcaccacctg
     3661 cgtttcgccc tgcgcatccc gcagggtcag gctgtgactc tgcgctgtca cccggtctat
     3721 cacatagcgg tcatgactgc gtgtctccgg gttccactgc tccatcacca tccccggtcg
     3781 gtacatatcc cgcagataac ggctccggct gtccagccag accggtgaca gtgcagtcat
     3841 ggtcacctca gggtgtccga gcacgccctg cgttttcagc tcactgcgaa tggcctctgt
     3901 cagtatggcc tgttcccgta ccccgctgac ctgtgccacg ctctcttctc cggctttcac
     3961 gctggccgca aaatctccgg ccagccgggc atagcggaca ttacggtccg gttcactgat
     4021 gatggtggcc ggtcgctgtt ctcccccctg ccagcgatat gtgttcaccc cggcatcctt
     4081 catggccatc agtgcactgc ctgtaccggt tcgctgcccg ctgtcggtta tcaggacctg
     4141 tacgttatga cgtgcggcac catccagcag ggttaacgtc tctttcaggg agagtttttc
     4201 gccctggtcg acgataacgg tactgcccgg cgggaaggcc atgccttcca gcaactgacg
     4261 acgtccggtt atcagttcac cggacagccg ttcatcctgc ttcaggttca tctgcgagcg
     4321 acggtcagca gcgataatct gcacctcccg cccctgctcc cgggccatca tgaccagttc
     4381 agccacccgc tcacgctgcc cggctgcacc gccctgcccg gacacaatgg ccagcgacgg
     4441 gcgatcctgt gccagaacac tgacggcatc gctgtaaccg gccgtccggg ggacactttt
     4501 ctccggatgt acggtcaccc ggttctgttt catgatgtca cgactgagtg cccggactga
     4561 cagctcatcg agcacatgaa tcccggacgt gaacagcccc ttctcacgat cgagggggat
     4621 aagctgctca cggctgatgg cctcatcgat accggcgcgt gcccgttcaa tcacaccatt
     4681 ttccggcggc agtatgccga ccgtcctggc cagcacgtcc gtatacgtga actgcacttt
     4741 tcgttcactt aatccggcaa tggcctgtgt caccgcctgc tgcacatccg gcccgtcctg
     4801 tgaagcaggc ccgggcgcct gcgtgcggat ctccgcacgc tgatccgccg cgtcacgata
     4861 tgcccggatg tcgaacccgg tttccttcag cgtctgcatc cattcagcca ttctgacttc
     4921 aggatcgacg tgctgtttgg atttacgcgt atccagtgcc gccacatccc gggatttcag
     4981 cgaggcgtct tcaccgacgg cctcccggat agcctgtgag cgtccggaga aggcttccac
     5041 cggtacgccc ggcatctccc acataccgtg cttaccgacc acttcggttt cataacccag
     5101 cgcctcaacg cgctgacgta actcactctg atatattttc ccgaacgcaa tccggttcgc
     5161 gagcacgttc tcggaaaatc cggttttccc cactttgtca ctgctcagtg tcttccattc
     5221 gccgttatgc tgcgtgacat tagccaccac cacatgcgtg tgtaactgtg ggtcctgatc
     5281 gcgactggtg tcatggttaa acagtgccat caccagatta ccggtcagca ccgtttctga
     5341 ctgtccgtcc gtcatcaccc gtgtggaagc cagcgcctcc acctgacgga cagcaaaatc
     5401 cacagcctgg ttatgtgcat caataagacg tttatccccc ccgagcatgg ccatcatgga
     5461 gacacttttg ggggcggaga aggtcaggtc gtagccggga cgatgcttat tactgccatc
     5521 ctgcatgcgg cttaaatccg ccccgtccgg cagcctgccc tccagaagac gggtaaaaac
     5581 atccttatcg acgctgccct gcagcccaag ctgttcagcc ccctgaccgg cccagcgttc
     5641 tcccatgctg cccagcacat agtaattatc cttgtcggtg taatagtttc cggcactgcc
     5701 tgccgattta acaacgctga aactcaacat cagaaatcat ctcccggctc aacatcctcc
     5761 ccgcgctccc ggtgcacatt aatgttcacc tcttcacgac gctgcatctg ctgctggatg
     5821 tccggatgat tttcctgttg ccatgcctca taagcggcca tatccaccac ttcaccggat
     5881 tcactgatcc cgggtggcag ttgctgttcc atctcttctt ccggtttcat tttcagctcc
     5941 tgctcgatcc cccctgccgg aacactcaca cctgcatctg acttcttatc attgatgaca
     6001 gaagacactg gctgttgtgg ctgttgtggc tgttgtggct gttgtggctg ttgtggctgt
     6061 tgtggctgtt gtggctgttc agcctgagtc acgccttctc cggaggcaac ctccggttcg
     6121 aagaggctgg ccatctggcg accttctgct tcccttgcag caagtacggc actcagacgg
     6181 ttctccattt ccgggttgat gtcacgcgga ataaactccg gagcgacctt cggtcgtgcc
     6241 tgatatttca gggagagttt tactgccgga tacggtccgg gcagagtgac atagcaggtc
     6301 agatccggca gagactgaat gtcggaataa ctgaccagtg tctgacgctc catatcctta
     6361 ccggtcgata ccccgtcacg taccgggtca gcaccgtagg aatactgctc gctggctttc
     6421 aggtgctctt tctcaccaat ctcaccggca gcgaactctg caatcttatg gctcggggaa
     6481 cggaaaaaag cacgggtgtt catgacgtca aacagcgtgg ccgccgcttt ctcaccgtag
     6541 atatcttcca gctgggcata ggactggata ccaaacacat aacagccacc gaacttacgg
     6601 gcttccggca ggatctccac caggtccggc agtttgtgta acgtgggtaa ctcatcacag
     6661 aaaaaccaca cacgacggtt acggttttct cccattgcca gcagaccacg aatggcaatg
     6721 gacagccaca tggagatcac aggcttcagg gaggcatggg tgtcggcgtt cgacgaaata
     6781 aacagccagc cgtttttctg atcttcccgg acaccacgca tccagtcacg gatggtgaag
     6841 ggctcaccgt tatgctcaat cccctgcagg taacggatgg ctttcacgta gttggtcagc
     6901 acagcacgga tggaaatcgc cgttttctca attttctctt ccaccaggtt ggccgccggt
     6961 gaattacgca ggaaggtacg cagtttttca attttgatgg aaagcagtgt gtccaccagt
     7021 ttgctgtagc tgcggttggg gtcattacgc atcaggtacg ccgcttccgc aaaaatggta
     7081 cgtcctgaac cctgccagaa cgggtcctct tttgtaccca tagggatcag agtatttgcg
     7141 gtattatcaa aatccggctg tgtcaggcac tccttccaca aatcccatgc ggcacagcgg
     7201 gcatccagcg gattcaggat tttatcgatg gagggatcat agtaactttt aacaaattcc
     7261 cctgaacggt cataaatcac caccatatca ccgcgctgac gggcatagtt ggccagacga
     7321 cggataacct ccgacttacc ggccccgacc gtgccgtgca gacagaagtt ctggatttcg
     7381 gaatcccgga taatcggcag gtcgccaatc cggatagctt tccattgcac tgcgcatacg
     7441 gctggccatc atggaatcct gtgcactcat ggtgctgtca gcgccaccgg tgacagaatc
     7501 actgctcccc cggttagaac caaactgact gagcgtgttc caggcactgg caatactgct
     7561 gctgaaacca tgcagggcac tttctgctct gttcgacgcc tcccgggcca tttcctgttg
     7621 tgccgctgca atctgacgcg ttgcattgat accaaccggt aaccgggaca tcgctccgct
     7681 tgcatccatc accatattac cgtcacgggt ctgtgtggca gttgcgccac tgccggtctg
     7741 gtacatcatc tgaccaaacg acgtggtgct gttggtgctc cagctgaagc cgttcacgtt
     7801 ttccgtctgc atgttgccgt aggagtaatt accgtcaacc acactgcctg ctgcactggc
     7861 cgtcggactg atagaggaag aggcgaagtg gctgtacacg ctggaaaaac ctgcgccaag
     7921 gccttttacc atcatccatg acaacggggg gatcatggct gaaagatacc cggcagtaga
     7981 ggccaggtca gagtatttca gctgtatctg agagagttca gacagcacga ccggcgcacc
     8041 attcatcttc gcatagaatg tcatggcact gttcagaatg gcatacaaca acggccaggt
     8101 ctgcagccac atcagggcaa acacatagcc tttcaggacg gacagtgtca gtttgttgaa
     8161 gacagctgcc agtaccagaa gcggtaaaat accaatggca attcccgtca ggatagtctg
     8221 taccattggc aggtttcgca ttgtcacatg accgatagag acatgcgcca gacgttgctt
     8281 ctccatcgat gacgtggtgg ccagattcac caggctggcg gtgtcaccat tacgggcggc
     8341 attactggtg ataccctctt tcagggcatt tattgtgacg ttctggcgca taatctggct
     8401 ggcagactga ctggagccgt agaaataact gtaactgtca ctgaccagtt gtctgaataa
     8461 caggtccggg tccggtctgc cgccaaatat ctgctgcaca taataatgcc aggtcttgcc
     8521 tcccgtcttt gtatcgagat tcagcctgtc tttcagcgtg accgacgcat ccttacaggt
     8581 tatgaaatta ttattgttgt cataaacgcc ccgtagcggg ctgggtcggg aaaaaatcag
     8641 cgtgtagggg tcagcggagg ccatcagatc ttccagcgtg tatttatgat tcaggtaaat
     8701 atcacccagc acgcagttct ggacgtagtc ctggaacaga ttgatgattt ccggattccg
     8761 ggacaggaaa tcggtgcttt tcacaatcag attcgccccg aacagcatgc ctgtcttgct
     8821 gtaggtgacg ctgtccggtt gggtgaatat catctcgtag ctgaccacca ttgcatgccc
     8881 gatacgtgtc gtcagtgaaa gtggcatcgc cagaccgacc ggcacattat ccacccgatg
     8941 gactttgacc aggtcactgt tatcaataat ctgcaccgat gtacggacat taaccagcag
     9001 gctgataagc acaaacacgg ccaccgtgga ttcattaccg gtgactgagg cattggcatc
     9061 cgccccttca atcagcatgg ctttcgcaaa acttcctgac ggaatgtacg gcagcgatgg
     9121 tccctgtttt ccctcattac gggtaaacac cttacgctgt aacgggcttt tccgtcgggt
     9181 gatgacgatt gccagaatat ttccagccca gcacgtccgg cagcgggttt tgtggcagac
     9241 gtggacggcc ttttgccagg atgtaggcgc attcgtggcg atagcccaca tatgcggcct
     9301 tcgatgtgta gtttttggtg aagaccaggt gaccaacaac gctgaatccc gcatttttcc
     9361 aggcggcca
//
LOCUS       DAMZFT010000181         9327 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.181, whole genome shotgun sequence.
ACCESSION   DAMZFT010000181 DAMZFT010000000
VERSION     DAMZFT010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9327)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9327)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9327
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.181"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(9..2069)
                     /locus_tag="PN977_004570"
     CDS             complement(9..2069)
                     /locus_tag="PN977_004570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HDI6044514.1"
                     /translation="MSKAGKITAAISGAFLLLIVVAIILIATFDWNRLKPTINQKVSA
                     ELNRPFAIRGDLGVVWERQKQETGWRSWVPWPHVHAEDIILGNPPDIPEVTMVHLPRV
                     EATLAPLELLTKTVWLPWIKLEKPDARLIRLSEKNNNWTFNLGNDDNKDANAKPSAWS
                     FRLDNILFDQGRIAIDDKVSKADLEIFVDPLGKPLPFSEVTGSKGKADKEKVGDYVFG
                     LKAQGRYNGEPLTGTGKIGGMLALRGEGTPFPVQADFRSGNTRVAFDGVVNDPMKMGG
                     VDLRLKFSGDSLGDLYELTGVLLPDTPPFETDGRLVAKIDTEKSSVFDYRGFNGRIGD
                     SDIHGSLIYTTGKPRPKLEGDVESRQLRLADLGPLIGVDSGKGAEKSTRSEQKKGEKS
                     VQPAGKVLPYDRFETDKWDVMDADVRFKGRRIEHGSSLPISDLSTHIILKNADLRLQP
                     LKFGMAGGSIAANIHLEGDKKPMQGRADIQARRLKLKELMPDVELMQKTLGEMNGDAE
                     LRGSGNSVAALLGNSNGNLKLLMNDGLVSRNLMEIVGLNVGNYIVGAIFGDDEVRVNC
                     AAANLDIANGVARPQIFAFDTENALINVTGTASFASEQLDLTIDPESKGIRIITLRSP
                     LYVRGTFKNPQAGVKAGPLIARGAVAAALATLVTPAAALLALISPSEGEANQCRTILS
                     QMKK"
     gene            complement(2139..2906)
                     /gene="pdeH"
                     /locus_tag="PN977_004571"
     CDS             complement(2139..2906)
                     /gene="pdeH"
                     /locus_tag="PN977_004571"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709303.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase"
                     /protein_id="HDI6044515.1"
                     /translation="MIRQVIQRISNPEASIESLQERRFWLQCERAYTWQPIYQTCGRL
                     MAVELLTVVTHPLNPSQRLPPDRYFTEITVSHRMEVVKEQIDLLAQKADFFVEHGLLA
                     SVNIDGPTLIALRQQPKILRQIEDLPWLRFELVEHIRLPKDSTFASMCEFGPLWLDDF
                     GTGMANFSALSEVRYDYIKIARELFVMLRQSPEGRTLFSQLLHLMNRYCRGVIVEGVE
                     TPEEWRDVQNSPAFAAQGWFLSRPSPIETLNTAVLAL"
     gene            3138..4067
                     /gene="kdgK"
                     /locus_tag="PN977_004572"
     CDS             3138..4067
                     /gene="kdgK"
                     /locus_tag="PN977_004572"
                     /EC_number="2.7.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygluconokinase"
                     /protein_id="HDI6044516.1"
                     /translation="MSKKIAVIGECMIELSEKGADVKRGFGGDTLNTSVYIARQVDPA
                     ALTVHYVTALGTDSFSQQMLDAWHGENVDTSLTQRMENRLPGLYYIETDSTGERTFYY
                     WRNEAAAKFWLESEQSAAICEELANFDYLYLSGISLAILSPTSREKLLSLLRECRANG
                     GKVIFDNNYRPRLWASKEETQQVYQQMLECTDIAFLTLDDEDALWGQQPVEDVIARTH
                     NAGVKEVVVKRGADSCLVSIAGEGLVDVPAVKLPKEKVIDTTAAGDSFSAGYLAVRLT
                     GGSAEDAAKRGHLTASTVIQYRGAIIPREAMPA"
     gene            4162..5367
                     /locus_tag="PN977_004573"
     CDS             4162..5367
                     /locus_tag="PN977_004573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001517302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3142 domain-containing protein"
                     /protein_id="HDI6044517.1"
                     /translation="MPSCKRIFAASLLLICLGYGLHILFTPSQPLRFDQQVYIWQRVW
                     TAEHNEALKRSRPLFSTLRVLGMQFHPQEGIRHFTVNTSLIKQDGRPVWLVARLEGQL
                     TRLNNQQIIQHIQQTLREWQKAGITLVGVEIDYDAPTAKLPDYLTLLSTLHQALPTNI
                     KLSITVLPTWLESPHLARLLRIADTSVLQVHNVLSPEKGLLDASLAQRWVKQYARQTT
                     HPFFIALPAYGSALTSDNRVESEAPLYVAEEMRELSVSPQTIADFIQYLEQQPPHGLQ
                     GLVWFRLPLAGDRRAWSLTTLEAVIQHQPLTPRWSLIITQHADKQLFDLAFKNSGVID
                     APLPKQIIMSNADCLFADGASHYQVQPGPSSLNFVRGSARQLRAGESSPLGWARCNQK
                     IQGEFNVIP"
     gene            5354..7396
                     /locus_tag="PN977_004574"
     CDS             5354..7396
                     /locus_tag="PN977_004574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016242561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6044518.1"
                     /translation="MLSPKISRLSVIVFLSLISANVNACGPFIPNHLLTDRNSALLEM
                     PNGSFVFEVQQLAKRDPDLPIWHEDTPAEATQANTDDDAEPPAQNRILEITQKLQKPL
                     SKAQRLYYSGAKDFHQGKYDSPYFQQLLALPKTEQGEWRLATLYSLARGGFDATDDNL
                     DLIINKGGFNQELAEQAIQRYQQIIEEVRNGAADPELLSLASLGQIGHYYLKKGDVAA
                     AVKMYARQAAQGSPSGKASLRMISLYITREKNLPLLESVINDSLVQQLVIAELFIRHD
                     WGYVSEACTKDLVDYTCPTHRDRLIKLLSQHRVKGFSYSDRLAALAYRAGNYSLAQTL
                     LQDAPESGLSEWLKAKMALRAGDIPLATRHYARAAPLFSTDEVWGSMDPESPYYHADK
                     VTIPSCRIASEQAIVALKRDDYLQATALFYQAKDLYWADLAWVAENVLTLSELHAFVE
                     KNVPAPQHPLKPIDLNKSSYGYYISPDIIPTDVKLRALLGRRLIRNGQYQQAINWFDI
                     PDHRQAAQKLADLMAIAKNKNIPDKQRAQAQYEAARLLRFSGIELVAYEMYPDNAISS
                     GQYSALFEYRNNDQWISRKEKQRIRHAIPTADDHFLHYRWQAAEWAGQSADLLHPKTQ
                     AWAAVLCHAASWVRYRDTETGALIYQRYVKNGKVFDWSAHFGRDCPDPVFKKDLKK"
     gene            complement(7374..7904)
                     /locus_tag="PN977_004575"
     CDS             complement(7374..7904)
                     /locus_tag="PN977_004575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001269541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6044519.1"
                     /translation="MRYKVTLFLLTMILCLTGFYSCNKNFLLLWDANNMYVSTRNNID
                     KDKVKIEFGISVNTINRETDAELFTDRAKYRIIFDGNLKNRMINEYGENDFLITYDDR
                     CYLSFRQFKTNRRHQHDYYFDFFNNNGNVFVTVEIKGENPLKFTRSLNDMRQQFSPTR
                     EDSPSHSCKVISSDPS"
     gene            complement(8087..>9327)
                     /locus_tag="PN977_004576"
     CDS             complement(8087..>9327)
                     /locus_tag="PN977_004576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HDI6044520.1"
                     /translation="TQSGGLDAAQARSLWQQGIDPKRPMPPVIVSYDTTLFNLSLPNN
                     RNDLLKEALSYLANATGKLTITPETINHALQSQDMVATWPADTKEGWWRYRLKGSTLL
                     GHDPADPLKQPVEAEKIKDFYQKWYTPDAMTLLVVGNVDARSVVDQINKTFGELKGKR
                     ETPAPVPTLSPLRAEAVSIMTDAVRQDRLSIMWDTPWQPIRESAALLRYWRADLAREA
                     LFWHVQQALSASNNKEIGLGFDCRVLYLRAQCAINIESPNDKLNSNLNLVARELAKVR
                     DKGLPEEEFNALVAQKKLELQKLFAAYARADTDILMGQRMRSLQNQVVDIAPEQYQKL
                     RQDFLNSLTVEMLNQDLRQQLSNDMALILLQPKGEPEFNMKALQAAWDQIMAPSTAAA
                     ATSVATDDVHPEVTDIPPAQ"
BASE COUNT         2335 a         2423 c         2238 g         2331 t
ORIGIN      
        1 aattatcatc acttcttcat ctgcgacaaa atcgtccgac actgattagc ctccccttcg
       61 gaaggagaga tcagcgccag taacgccgcc gctggcgtaa ccagcgttgc cagtgccgcc
      121 gcgacggctc cgcgagcaat caacggtccg gcttttaccc cagcctgcgg atttttaaac
      181 gtcccacgca catacagcgg cgaacgcagt gtgataatcc gaatcccttt actttctgga
      241 tcgatagtca aatccagctg ttccgaagca aaacttgccg tgccggtaac gttaatcaac
      301 gcgttctccg tatcgaaagc aaagatctgc gggcgcgcca cgccgttggc aatatcaaga
      361 ttcgccgccg cgcagttcac ccgcacctca tcgtcaccaa atatcgcacc gacaatgtag
      421 ttgccgacat tcagcccgac aatctccatc aggttgcggc tcaccagccc gtcattcatc
      481 aacagtttca ggttgccgtt actgttgcct aaaagcgccg ccaccgagtt accgctaccg
      541 cgtagttccg cgtcaccgtt catttccccc agcgtcttct gcatcagttc tacatcgggc
      601 atcagttctt tcagtttcag tcgacgagcc tgaatatctg cccgcccctg catcggcttt
      661 ttatcgcctt ccagatgaat attcgccgca atgctgccgc ccgccatgcc aaatttcagc
      721 ggttgcaggc gcaggtcagc atttttgagg atgatatgag tagaaagatc gctaatcggc
      781 aggctactgc catgttcaat gcgccgccct ttgaagcgaa catcggcatc catgacatcc
      841 catttatcgg tttcgaagcg gtcataaggc agcactttgc ccgcaggctg aacgcttttt
      901 tcacccttct tctgttcaga ccgtgtcgac ttttcagctc ctttcccgga atcaacgcca
      961 atcaacggtc ccaggtccgc cagccgcaat tgccgcgact cgacatcacc ttccagtttt
     1021 ggtcgtggct tgccggtggt gtagatcaga gaaccgtgga tatcgctatc gccaatgcgc
     1081 ccattaaaac cgcgataatc aaagaccgac gatttttcag tgtcgatttt cgctaccagc
     1141 cgaccatccg tttcaaacgg cggggtatcg ggcagcagaa cgcccgtcag ttcatagaga
     1201 tcacccagcg aatcgccaga aaatttaagc cgtaaatcga caccccccat cttcattggg
     1261 tcattcacaa cgccatcaaa agcaacacgg gtgttaccag agcggaaatc agcctgtacc
     1321 ggaaacggcg taccttcgcc acgcagcgcc agcataccgc ctatttttcc cgtaccagtg
     1381 agcggttcac cgttataacg tccctgcgcc ttcaggccaa aaacgtaatc gcccaccttt
     1441 tctttatccg ctttaccttt cgatccggta acttcgctga acggcagcgg cttgcctaac
     1501 ggatcgacaa aaatttccag atccgctttg cttactttgt catcaatggc gatccgccct
     1561 tgatcgaaca gaatattatc cagccgaaac gaccacgccg acggctttgc attcgcgtct
     1621 ttgttgtcat cgttgccgag attaaacgtc cagttattgt tcttttcgga gaggcgaatc
     1681 aggcgcgcgt cgggcttttc gagcttgatc cacggcaacc agaccgtttt ggtcagcagc
     1741 tccagcgggg ccagcgtcgc ttctactcgc ggcaaatgca ccattgtgac ttcgggaata
     1801 tccggtggat tgccaagaat gatgtcttcc gcgtgtacat ggggccacgg tacccagctg
     1861 cgccagccag tttcttgttt ttgccgctcc cacaccacgc ccagatcgcc acggatagcg
     1921 aacggacgat tcaactccgc agagactttc tggttgatgg tcggtttgag tcgattccag
     1981 tcaaaagttg caatcaaaat gatcgccacg acaatcaaca acaagaaagc ccctgaaatc
     2041 gcagcggtta ttttgcctgc cttgctcatc gttcgcttcc tcatattctt cctgtgccag
     2101 tcctaaagat agtccagcca ggcggaaaat gaggcagctt atagcgccag aaccgccgta
     2161 ttcagcgttt ctatcggtga cgggcgtgaa agaaaccagc cttgtgcggc gaatgcgggc
     2221 gagttctgaa catcacgcca ctcttccggc gtttctacgc cctcaacaat caccccgcga
     2281 caatagcgat tcatgagatg taaaagctga gaaaagagtg tgcgtccttc cggtgactga
     2341 cgcagcatca caaacagttc tcgcgcgatt ttgatgtagt cataacgcac ttcacttagt
     2401 gcagagaaat ttgccatccc ggtaccaaaa tcatccagcc acagcgggcc aaattcacac
     2461 atcgaggcaa aggttgaatc tttcggcagg cggatgtgtt ccaccagttc gaaacgcagc
     2521 cagggaagat cctcaatctg gcgcaggatt tttggttgct gacgcagggc gatgagcgta
     2581 gggccatcaa tattgaccga tgccagcagg ccgtgctcta caaagaagtc ggctttttgc
     2641 gccagcaaat caatctgctc tttcacaacc tccatccgat ggctaacggt gatttcagta
     2701 aaatagcgat ccggcggcag gcgttgcgaa gggttcaagg gatgcgtgac caccgttaat
     2761 agctccacgg ccattaaccg cccgcatgtt tgatagatcg gctgccaggt gtaagcacgc
     2821 tcacactgca accaaaaacg ccgttcctgc aagctctcga tgcttgcttc agggttgctt
     2881 attcgctgga taacctgcct tatcatcaaa gatgtcctgt gtttaaaagt gatgctgccc
     2941 ggactcgaca aagattatcg gcgcgcaagg gcagaacttt accgcctggc cggggtaaat
     3001 ttccccactc cacaacggaa tctttcagga atacgttctg gctcaaaaaa ataatggaac
     3061 actgttttaa tatggttgac cagcaaacca ccacagcgca aactaacgct aattttttac
     3121 agatcaggtt cacgactatg tccaaaaaga ttgccgtgat tggcgaatgc atgattgagc
     3181 tttccgagaa aggcgcggac gttaagcgcg gtttcggcgg cgataccctg aacacttccg
     3241 tctatatcgc ccgtcaggtc gatcctgcgg cattaaccgt tcattacgta acggcgctgg
     3301 gaacggacag ttttagccag cagatgctgg acgcctggca cggcgagaac gttgatactt
     3361 ccctgaccca acggatggaa aaccgtctgc cgggccttta ctacattgaa accgacagca
     3421 ccggcgaacg tacgttctac tactggcgta acgaagccgc cgccaaattc tggctggaga
     3481 gtgagcaatc cgcggcgatt tgtgaagagc tggcgaattt cgattatctc tacctgagcg
     3541 ggattagcct ggcgatctta agcccgacca gccgcgaaaa gctgctttcc ctgctgcgcg
     3601 aatgccgcgc caacggcgga aaagtgattt tcgacaataa ctatcgtccg cgcctgtggg
     3661 ccagcaaaga agagacacag caggtgtacc aacaaatgct ggaatgcacg gatatcgcct
     3721 tcctgacgct ggacgatgaa gacgcgctgt ggggtcaaca gccggtggaa gacgtcattg
     3781 cgcgcaccca taacgcgggc gtgaaagaag tggtggtgaa acgcggggcg gattcttgcc
     3841 tggtgtccat tgctggcgaa gggttagtgg atgttccggc ggtaaaactg ccgaaagaaa
     3901 aagtgatcga taccaccgca gctggcgact ctttcagtgc cggttatctg gcggtacgtt
     3961 tgacgggcgg cagtgcggaa gacgcagcga aacgcgggca cctgaccgca agtaccgtta
     4021 ttcagtatcg cggcgcgatt atcccgcgtg aagcgatgcc tgcgtaatgg tttttgacgg
     4081 agaacgggta gatattcgtt ctccgttaat agtttcgtcg gtatactatc agcttgttga
     4141 taatcgtaat tccgtaacaa tatgccatct tgcaaaagga tttttgcagc ttcacttctt
     4201 ctgatctgcc tgggctacgg gctgcatatt ttatttaccc cttcacaacc gcttcgcttt
     4261 gatcagcaag tctatatctg gcaacgcgtc tggacggcag aacataacga ggcactcaaa
     4321 cgaagccgcc cgcttttctc tacgcttcgc gtactcggga tgcaatttca cccgcaagaa
     4381 ggcatccgac actttacggt taatacttca cttattaagc aagatggtcg cccggtctgg
     4441 ctggtcgcgc gactcgaagg tcagcttaca cggcttaata accagcaaat tatccagcat
     4501 attcaacaaa ccctacgtga gtggcaaaaa gctggtatta cgctggtagg ggtagaaatt
     4561 gattatgacg cgccaacagc aaaattgccg gactatctga ccctacttag tacgttacat
     4621 caggcacttc ctacaaatat aaaacttagc attaccgtcc tgcctacctg gcttgaatca
     4681 ccacatctcg cccgattatt gcggattgcg gacacttcgg ttttgcaggt acataacgtg
     4741 ctatcgcccg aaaaaggcct gttagatgcc agtctggcgc agcgttgggt aaagcaatat
     4801 gccagacaaa ccacgcatcc ttttttcatc gcattgcccg cttacggttc tgcgctcacc
     4861 agtgataatc gcgtcgagag tgaagctccg ttatacgttg ccgaagagat gcgtgaatta
     4921 agtgtgtcgc cgcaaacaat tgccgatttc attcaatatt tagaacagca accaccgcat
     4981 ggattacagg ggttggtatg gtttcggcta ccattagcag gcgatcgccg cgcctggtcc
     5041 ctgacaacgc ttgaagctgt gattcaacat caaccgctga ctccgcgctg gtcgttaata
     5101 attactcaac atgcagacaa gcaacttttt gatctggcct ttaaaaacag cggcgtaatt
     5161 gatgcgccat tgccaaaaca aataattatg tccaatgcag attgtctatt cgctgatgga
     5221 gccagccact atcaggtgca acctggacca tccagcctga attttgtgag aggaagcgcc
     5281 aggcaactac gcgccggaga aagcagtccg ctgggatggg cgcgctgcaa tcaaaaaatt
     5341 caaggagagt ttaatgttat cccctaaaat atcgcggctt tccgttattg tatttctctc
     5401 actaattagc gccaacgtta atgcctgtgg accatttatt cctaaccatc tgttaactga
     5461 ccgcaatagc gcactgctgg aaatgccaaa tggcagtttt gtctttgaag tacaacagtt
     5521 ggctaaacgc gatcctgatt taccgatatg gcatgaagat acgcccgcgg aggcaacgca
     5581 ggcaaataca gatgatgatg ctgagcctcc tgcgcaaaat cggatactgg aaatcacgca
     5641 aaaattgcag aagccattat ctaaggcgca gaggctttat tattcaggag ctaaggattt
     5701 tcatcaggga aaatacgata gcccttactt tcagcaatta ttagccttgc cgaaaacaga
     5761 gcaaggggaa tggcgtcttg ctacgctata tagtctggca agggggggat ttgatgccac
     5821 tgacgacaat ctcgatctta taatcaataa aggcggcttt aatcaggaac ttgctgagca
     5881 ggccatacag cgttatcagc aaatcattga agaagtgcgt aatggagcag ccgatcctga
     5941 attgctctct ctcgccagtc tcggtcagat tgggcattat tatctgaaaa aaggtgatgt
     6001 cgctgcggca gtaaaaatgt atgcccggca agcggcccag ggttcgccct ccgggaaagc
     6061 gtcgctacgg atgatttcgc tgtatataac cagggagaaa aatttaccac tgctggagtc
     6121 ggttattaat gattctctgg ttcagcaact ggtaatcgca gagctcttta ttcgtcatga
     6181 ctgggggtat gttagcgaag cctgtactaa ggaccttgta gattatacct gtccaacgca
     6241 tcgcgaccgt ttaattaagt tgctaagtca acacagagtt aaaggattta gttatagcga
     6301 tcgcctggca gcactggcgt atcgggcagg aaattattcg ttagcacaaa cgctgttgca
     6361 ggatgcgcca gaaagtggat taagcgaatg gttaaaagca aaaatggcat tacgagccgg
     6421 agatattcca ctggcaacgc gtcactatgc tcgcgcagcc ccgctgttct ctaccgatga
     6481 agtctgggga agtatggacc cggaaagccc ttattatcat gccgataaag ttaccatccc
     6541 gtcttgccgt attgccagtg aacaggcgat tgtggcatta aaacgcgatg attatcttca
     6601 ggccaccgca ttattttacc aggccaaaga tctctattgg gctgatttag cctgggtggc
     6661 agaaaacgtt ttgaccttgt cagagttaca cgcgtttgtt gaaaaaaacg taccagcacc
     6721 tcagcatcct ttaaaaccta tagatttaaa caagagcagt tacggttatt atatttcgcc
     6781 agatatcatt cccacggatg ttaaattacg tgcactatta ggtagacgat taatacgtaa
     6841 tggtcagtat cagcaggcca tcaattggtt tgatattccc gatcatcgcc aggccgcaca
     6901 aaaactggct gatttaatgg cgatagcaaa aaataaaaat atccctgata aacaacgcgc
     6961 ccaggcgcaa tacgaagctg cgcggctttt acgtttcagt ggtatcgaat tggtggctta
     7021 tgaaatgtat cccgataatg ctatttccag tgggcagtat tcagcactct ttgaatacag
     7081 aaataacgat cagtggataa gtcgtaaaga aaaacaacgt attcgccatg caatccccac
     7141 cgctgatgat cattttttgc attaccgctg gcaggctgcg gaatgggccg gacaatctgc
     7201 cgatttactt caccctaaaa cccaggcttg ggctgccgtg ttatgccatg ctgcaagttg
     7261 ggtcagatat cgtgatacag aaaccggtgc attaatttat caacgctacg tgaaaaacgg
     7321 taaagttttt gactggtctg cgcattttgg tcgtgattgt ccagatccag tgtttaagaa
     7381 ggatctgaag aaataacctt gcaggagtga gaagggctat cctcacgagt tggtgaaaac
     7441 tgctgacgca tatcatttaa actacgcgta aatttaagtg ggttctcgcc tttgatttcg
     7501 actgtgacaa agacattccc attgttgtta aagaagtcaa aataatagtc atgttgatga
     7561 cgacgattgg ttttaaattg acgaaatgat aaatagcagc ggtcatcgta ggtaattaaa
     7621 aaatcatttt cgccgtactc atttatcatc ctgtttttta ggttaccatc gaagataatt
     7681 ctatacttcg ctctgtcagt aaataattcg gcatccgtct ctcggttgat cgtgttgacg
     7741 ctgataccga attctatttt taccttgtct ttatcgatat tatttcttgt gctgacatac
     7801 atattattgg catcccagag taagaggaag ttcttgttgc aggaatagaa ccctgttaag
     7861 cagagaatca tagtcagcaa gaacagtgtg actttgtagc gcattcatat tcctttagca
     7921 cgttacgttc acaaagtgtg gttacttcct tttctataaa ccacagcagt cagtaattat
     7981 ctgcacgcta ccgccgtgcc gggaaaaatc tctgcgggta tatcacggta acttattaat
     8041 taccgttaat cccggcagcg cagaagaaac gctgccgggt tatttatcac tgtgcgggtg
     8101 gaatatccgt cacttcagga tgtacgtcat ccgtggcgac agatgtggcg gcagccgcag
     8161 tcgatggggc catgatttga tcccaggccg cctgcaacgc tttcatgtta aattccggct
     8221 cgcctttcgg ctgcagcagt attaacgcca tatcattcga caactgctga cgcagatcct
     8281 gatttaacat ctccaccgtc aggctgttaa ggaaatcctg acgcagtttc tgatattgct
     8341 ccggcgcgat atcgacgacc tgattttgca acgaacgcat ccgctgcccc atcagaatat
     8401 cggtatcagc tcgcgcatag gcggcaaaca gtttctgcag ctccagtttc ttttgcgcca
     8461 ctaacgcatt gaactcttct tccggcagac ctttatcgcg aacctttgcc agttcacgcg
     8521 ccaccagatt aaggttgctg ttcagcttgt cgtttggtga ttcgatgttg atggcacact
     8581 gcgcacgcag atacagcaca cggcaatcaa atccaagacc gatttctttg ttgttactgg
     8641 cgcttaacgc ttgctgaacg tgccagaaca gcgcctcacg ggccaggtcc gcacgccagt
     8701 agcgcagcag tgcggctgat tcacgaatcg gctgccacgg cgtatcccac atgatagata
     8761 accggtcctg acgcaccgcg tcggtcataa tactcaccgc ttccgcacgc agcggagaaa
     8821 gcgtcggcac cggagccggt gtttcacgtt tgcctttcag ttcgccaaac gttttattga
     8881 tttggtcgac gaccgagcgc gcatccacgt ttcccaccac cagcagcgtc attgcatccg
     8941 gggtgtacca tttctgatag aaatctttaa tcttttccgc ttcaacgggt tgtttcagcg
     9001 gatcggcagg atcgtgacct aacaaggttg atcctttcag acgataacgc caccagccct
     9061 ctttagtatc ggcaggccag gttgccacca tgtcctgact ttgcagcgcg tggttgatgg
     9121 tttctggcgt gatagtcagt ttgccagtgg catttgccag ataagagagc gcttctttca
     9181 gcaagtcgtt acggttattg ggcaaactca ggttaaacag cgtggtgtca taagagacaa
     9241 ttaccggcgg catcgggcgt ttagggtcga tcccctgctg ccacaatgaa cgcgcctgcg
     9301 ctgcgtcaag gccaccgctt tgcgtta
//
LOCUS       DAMZFT010000182         9254 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.182, whole genome shotgun sequence.
ACCESSION   DAMZFT010000182 DAMZFT010000000
VERSION     DAMZFT010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9254)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9254)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9254
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.182"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            118..2469
                     /gene="polB"
                     /locus_tag="PN977_004577"
     CDS             118..2469
                     /gene="polB"
                     /locus_tag="PN977_004577"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase II"
                     /protein_id="HDI6044521.1"
                     /translation="MAQAGFILTRHWRDTPQGTEVSFWLASDNGPLQVTLAPQESVAF
                     IPADQVPRAQHILQGEQGFRLTPLALKDFHRQPVYGLYCRAHRQLMNYEKRLREGGVT
                     VYETDVRPPERYLMERFITSPVWVEGDIRNGAIVNARLKPHPDYRPPLRWVSIDIETT
                     RHGELYCIGLEGCGQRIVYMLGPENGDASALDFELEYVASRPQLLEKLNAWFATHDPD
                     VIIGWNVVQFDLRMLQKHAERYRIPLRLGRDNSELEWREHGFKNGVFFAQAKGRLIID
                     GIEALKSAFWNFSSFSLETVAQELLGEGKSIDNPWDRMDEIDRRFAEDKPALATYNLK
                     DCELVTQIFHKTEIMPFLLERATVNGLPVDRHGGSVAAFGHLYFPRMHRAGYVAPNLG
                     EVPPHASPGGYVMDSRPGLYDSVLVLDYKSLYPSIIRTFLIDPVGLVEGMAQPDPEHS
                     TEGFLDAWFSREKHCLPEIVTNIWHGRDEAKRQGNKPLSQALKIIMNAFYGVLGTTAC
                     RFFDPRLASSITMRGHQIMRQTKALIEAQGYDVIYGDTDSTFVWLKGAHSEEEAAKIG
                     RALVQHVNAWWAETLQKQRLTSALELEYETHFCRFLMPTIRGADTGSKKRYAGLIQEG
                     DKQRMVFKGLETVRTDWTPLAQQFQQELYLRIFRNEPYQEYVRETIDKLMAGELDARL
                     VYRKRLRRPLSEYQRNVPPHVRAARLADEENQKRGRPLQYQNRGTIKYVWTTNGPEPL
                     DYQRSPLDYEHYLTRQLQPVAEGILPFIEDNFATLMTGQLGLF"
     gene            2633..5539
                     /gene="rapA"
                     /locus_tag="PN977_004578"
     CDS             2633..5539
                     /gene="rapA"
                     /locus_tag="PN977_004578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001117023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-associated protein RapA"
                     /protein_id="HDI6044522.1"
                     /translation="MPFTLGQRWISDTESELGLGTVVAVDARTVTLLFPSTGENRLYA
                     RSDSPVTRVMFNPGDTITSHDGWQMQVEEVKEENGLLTYIGTRLDTEESGVALREVFL
                     DSKLVFSKPQDRLFAGQIDRMDRFALRYRARKYSSEQFRMPYSGLRGQRTSLIPHQLN
                     IAHDVGRRHAPRVLLADEVGLGKTIEAGMILHQQLLSGAAERVLIIVPETLQHQWLVE
                     MLRRFNLRFALFDDERYAEAQHDAYNPFDTEQLVICSLDFARRSKQRLEHLCEAEWDL
                     LVVDEAHHLVWSEDAPSREYQAIEQLAEHVPGVLLLTATPEQLGMESHFARLRLLDPN
                     RFHDFAQFVEEQKNYRPVADAVAMLLAGNKLSNDELNMLGEMIGEQDIEPLLQAANSD
                     SEDAQSARQELVSMLMDRHGTSRVLFRNTRNGVKGFPKRELHTIKLPLPTQYQTAIKV
                     SGIMGARKSAEDRARDMLYPERIYQEFEGDNATWWNFDPRVEWLMGYLTSHRSQKVLV
                     ICAKAATALQLEQVLREREGIRAAVFHEGMSIIERDRAAAWFAEEDTGAQVLLCSEIG
                     SEGRNFQFASHMVMFDLPFNPDLLEQRIGRLDRIGQAHDIQIHVPYLEKTAQSVLVRW
                     YHEGLDAFEHTCPTGRTIYDSVYNDLINYLASPDETEGFDDLIKNCREQHEALKAQLE
                     QGRDRLLEIHSNGGEKAQALAESIEEQDDDTNLIAFAMNLFDIIGINQDDRGDNMIVL
                     TPSDHMLVPDFPGLSEDGITITFDREVALAREDAQFITWEHPLIRNGLDLILSGDTGS
                     STISLLKNKALPVGTLLVELIYVVEAQAPKQLQLNRFLPPTPVRMLLDKNGNNLAAQV
                     EFETFNRQLNAVNRHTGSKLVNAVQQDVHAILQLGEAQIEKSARALIDAARNEADEKL
                     SAELSRLEALRAVNPNIRDDELTAIESNRQQVMESLDQAGWRLDALRLIVVTHQ"
     gene            5551..6210
                     /gene="rluA"
                     /locus_tag="PN977_004579"
     CDS             5551..6210
                     /gene="rluA"
                     /locus_tag="PN977_004579"
                     /EC_number="5.4.99.28"
                     /EC_number="5.4.99.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA pseudouridine(32) synthase/23S
                     rRNA pseudouridine(746) synthase RluA"
                     /protein_id="HDI6044523.1"
                     /translation="MGMENYNPPQEPWLVILYQDDHIMVVNKPSGLLSVPGRLEEHKD
                     SVMTRIQRDYPQAESVHRLDMATSGVIVVALTKAAERELKRQFREREPKKQYVARVWG
                     HPSPAEGLVDLPLICDWPNRPKQKVCYETGKPAQTEYEVVEYAADNTARVVLKPITGR
                     SHQLRVHMLALGHPILGDRFYASPEARAMAPRLLLHAEMLTITHPAYGNSMTFKAPAD
                     F"
     gene            complement(6328..7143)
                     /gene="djlA"
                     /locus_tag="PN977_004580"
     CDS             complement(6328..7143)
                     /gene="djlA"
                     /locus_tag="PN977_004580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlA"
                     /protein_id="HDI6044524.1"
                     /translation="MQYWGKIIGVAVALLMGGGFWGVVLGLLIGHMFDKARSRKMAWF
                     ANQRERQALFFATTFEVMGHLTKSKGRVTEADIHIASQLMDRMNLHGASRTAAQNAFR
                     VGKSDNYPLREKMRQFRSVCFGRFDLIRMFLEIQIQAAFADGSLHPNERAVLYVIAEE
                     LGISRAQFDQFLRMMQGGAQFGGGYQQQSGGGNWQQTQRGPTLEDACNVLGVKPTDDA
                     TTIKRAYRKLMSEHHPDKLVAKGLPPEMMEMAKQKAQEIQQAYELIKQQKGFK"
     gene            7398..>9254
                     /gene="lptD"
                     /locus_tag="PN977_004581"
     CDS             7398..>9254
                     /gene="lptD"
                     /locus_tag="PN977_004581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly protein LptD"
                     /protein_id="HDI6044525.1"
                     /translation="MKKRIPTLLATMIATALYSQQGLAADLASQCMLGVPSYDRPLVQ
                     GDTNDLPVTINADHAKGDYPDDAVFTGSVDIMQGNSRLQADEVQLHQKEAPGQPEPVR
                     TVDALGNVHYDDNQVILKGPKGWANLNTKDTNVWEGDYQMVGRQGRGKADLMKQRGEN
                     RYTILDNGSFTSCLPGSDTWSVVGSEIIHDREEQVAEIWNARFKVGPVPIFYSPYLQL
                     PVGDKRRSGFLIPNAKYTTTNYFEFYLPYYWNIAPNMDATITPHYMHRRGNIMWENEF
                     RYLTQAGAGLMELDYLPSDKVYEDEHPEDSNSRRWLFYWQHSGVMDQVWRFNIDYTKV
                     SDPTYFNDFDNKYGSSTDGYATQKFSVGYAVQNFDATLSTKQFQVFDDTSGNSYAAEP
                     QLDVNYYHNDLGPFDTRVYGQAVHFVNTNSNMPEATRVHLEPTINLPLSNTWGSINTE
                     AKLLATHYQQTNLDWYNSNPQNNKLADSVNRVMPQFKVDGKMVFERDMEMLAPGYTQT
                     LEPRAQYLYVPYRDQSDIYNYDSSLLQSDYSGLFRDRTYGGLDRIASANQVTTGITTR
                     VYDDAAVERFNISVGQIYYFTESRTGDDNITWENDDKTGSLVWAGDTYWRISE"
BASE COUNT         2209 a         2470 c         2493 g         2082 t
ORIGIN      
        1 ctatctgcgt aagcatggtg cgaaggcata ttacgggcag taatgactgt ataaaaccac
       61 agccaatcaa acgaaaccag gctataatca agcctggttt tttattggat tttcagcgtg
      121 gcgcaggcag gttttatctt aacccgacac tggcgggata ccccgcaagg aaccgaagtt
      181 tccttttggt tagcgtcgga caacgggccg ttgcaggtta cgcttgcgcc gcaagagtcc
      241 gtggcgttta ttcccgccga tcaggttccc cgcgcccagc atattttgca gggtgagcaa
      301 ggctttcgcc tgacgccgct ggcgttaaag gattttcatc gccagccggt gtatggcctt
      361 tactgtcgcg cccatcgcca gttgatgaat tacgaaaagc gcctgcgtga aggtggcgtt
      421 accgtctacg agaccgatgt gcgcccgcca gaacgctatc tgatggagcg gtttatcacc
      481 tcaccggtgt gggtcgaggg tgatatacgc aatggcgcta tcgttaatgc ccgtctgaaa
      541 ccgcatcccg actatcgtcc gccgctcagg tgggtttcta ttgatattga aaccacccgc
      601 cacggtgagc tgtactgcat cggcctggaa ggctgcgggc agcgcatcgt ttatatgctg
      661 gggccagaga atggcgacgc ctccgcgctc gattttgaac tggaatacgt cgccagtcgc
      721 ccgcagctac tggaaaaact caacgcctgg tttgcgactc acgatcctga tgtgatcatc
      781 ggctggaacg tggtgcaatt cgatctacga atgctgcaaa aacatgccga gcgttatcgt
      841 attccgctgc gtctggggcg tgataacagt gagctggagt ggcgcgagca cggctttaaa
      901 aacggcgtct tttttgccca ggccaaaggg cgactgatta tcgacggtat cgaggcgctg
      961 aaatccgcgt tctggaattt ctcttcattc tcgctggaaa ccgtcgctca ggagttatta
     1021 ggcgaaggaa aatctatcga taacccgtgg gatcgaatgg acgaaattga ccgccgtttc
     1081 gccgaagata agcctgcgct ggcaacttat aacctgaaag attgcgagct ggtgacgcag
     1141 atcttccaca aaactgaaat catgccgttt ttgctcgaac gggcgacggt gaacggcctg
     1201 ccggtagatc gacacggcgg ttcggtggca gcgtttggtc atctctattt tccccgtatg
     1261 catcgcgctg gttatgtcgc gcctaatctc ggcgaagtgc cgccgcacgc cagccctggc
     1321 ggctacgtga tggattcgcg acctgggctt tatgattcgg tgctggtgct ggactacaaa
     1381 agcctgtacc cgtcgatcat ccgcaccttt ctgattgatc ccgtcgggct ggtggaaggc
     1441 atggcgcagc ctgatccaga gcacagcacc gaaggttttc tcgacgcctg gttctcgcgg
     1501 gagaaacatt gcctgccgga gattgtgact aacatctggc acgggcgcga tgaagccaaa
     1561 cgccagggca ataagccact gtcgcaggcg ctgaagatca tcatgaatgc cttttatggc
     1621 gtgctcggga ctaccgcctg tcgcttcttc gatccgcgcc tggcatcgtc gatcaccatg
     1681 cgtggtcatc agatcatgcg gcaaaccaaa gcgttgattg aagcgcaggg ctacgacgtg
     1741 atctacggcg ataccgactc aacgtttgtc tggttgaaag gcgcacattc ggaagaagaa
     1801 gcggcgaaaa ttggtcgtgc actggtgcag cacgttaacg cctggtgggc agaaacgctg
     1861 caaaaacaac ggctgaccag cgcattagaa ctggagtatg aaacccattt ctgccgtttt
     1921 ctgatgccaa ccattcgcgg agccgatacc ggcagtaaaa aacgttatgc cgggctgatt
     1981 caggagggcg ataagcagcg gatggtgttt aaagggctgg aaaccgtgcg caccgactgg
     2041 acgccgctgg cccagcagtt tcagcaggag ctttacctgc gtatcttccg caacgagcca
     2101 tatcaggagt acgtccgcga aaccatcgac aaactgatgg cgggtgaact ggatgcgcgg
     2161 ctggtttacc gaaaacgcct tcgccgtccg ctgagcgaat atcagcgtaa tgttccgcct
     2221 catgtacgcg ccgctcgcct tgccgatgaa gaaaatcaaa agcgcggtcg ccccttgcaa
     2281 tatcagaacc gcggcaccat taagtacgta tggaccacca acggcccgga gccgctggac
     2341 taccaacgtt caccactgga ttacgaacac tatctgaccc gccagctaca acccgtggcg
     2401 gagggaatac tcccttttat tgaggataat tttgctacac ttatgaccgg gcaacttggg
     2461 ctattttgag caaaaaaaga gttcgccaga taccattttg atgcgtgacg aatgctttgc
     2521 catccagtac catagcgccc tttccattcc tggacctgaa taacaccact acctcataag
     2581 cacggtagcg ggtggttatt gcctgcaatt aaagatatag agccgaacac atatgccttt
     2641 tacacttggt caacgctgga tcagcgatac agaaagcgaa ttgggacttg gaaccgttgt
     2701 cgcggtggat gcgcgaactg tcactttact tttcccatct actggtgaaa accgtctgta
     2761 cgcacgcagt gattcccccg tgacccgcgt gatgttcaac cctggtgata ctattaccag
     2821 ccatgatggc tggcagatgc aagtcgaaga agtaaaagaa gaaaatggct tgctgaccta
     2881 tatcggtact cgcctggata ctgaagagtc cggcgtggcc ctgcgtgaag ttttccttga
     2941 tagcaaactg gtgttcagca aaccgcagga ccgtctgttt gccgggcaga ttgaccgtat
     3001 ggaccgcttt gcgctgcgtt atcgcgcgcg taagtattcc agcgaacagt tccgtatgcc
     3061 gtacagcggc ctgcgcggtc agcgtaccag cctgatcccg caccagctca acatcgctca
     3121 tgatgttggt cgccgccacg cgccgcgcgt cctgttggct gacgaagtgg gtttagggaa
     3181 aaccattgaa gccgggatga tcctgcatca gcaactgctc tctggcgctg ctgaacgtgt
     3241 gctgattatc gtcccggaaa ccttacagca tcagtggctg gtagaaatgc tgcgccgttt
     3301 caacctgcgc tttgctctgt ttgatgatga gcgttatgcc gaagctcagc acgatgctta
     3361 caacccgttc gataccgagc agctggtgat ttgctcgctg gattttgccc gtcgtagcaa
     3421 acagcgtctg gaacatctct gtgaagccga gtgggatcta ctggtggtcg atgaagcgca
     3481 tcacctggtg tggagcgaag atgcgccgag ccgtgaatat caggccattg aacaactggc
     3541 agagcacgtg ccgggcgttc tgctgctgac cgcaaccccg gaacagctgg ggatggaaag
     3601 ccacttcgcc cgtctgcgtc tgctggaccc gaaccgtttc cacgattttg cgcaattcgt
     3661 tgaagagcag aaaaattatc gtccggttgc ggacgccgtt gccatgctgc tggcaggtaa
     3721 caaactgagc aatgatgaac tgaacatgct cggtgagatg atcggcgagc aggatatcga
     3781 gccgctgttg caagcagcaa acagcgacag cgaagatgca cagagcgccc gtcaggagct
     3841 ggtttcgatg ctgatggatc gccacggcac cagccgcgtg ctgttccgta acacgcgtaa
     3901 cggtgtgaaa ggcttcccga aacgcgagct gcacaccatt aagctgccgc tgccgacaca
     3961 gtatcagacg gctattaaag tctccggcat tatgggcgca cgcaaaagtg cggaagaccg
     4021 cgctcgcgat atgctctacc cggagcgtat ttatcaggaa tttgaaggtg ataacgccac
     4081 ctggtggaac ttcgatccgc gcgttgagtg gctgatgggc tacctgacca gccatcgctc
     4141 tcagaaagtg ctggtgatct gtgccaaagc tgccactgcg ctgcaactgg agcaggtact
     4201 gcgcgaacgt gaaggtattc gcgccgcggt attccacgaa ggtatgtcga ttattgaacg
     4261 tgaccgcgct gccgcctggt ttgctgaaga agacaccggc gcacaggtac tgctgtgctc
     4321 ggaaatcggt tctgaaggac gtaacttcca gttcgccagc cacatggtga tgtttgacct
     4381 gccattcaac ccggatctgc tggagcagcg tattggtcgt ctggatcgta tcggtcaggc
     4441 gcacgatatt cagatccatg tgccttatct ggagaaaacc gctcagtcgg tgctggtgcg
     4501 ctggtatcac gaaggtctgg atgcattcga gcacacctgc ccgaccggac gcactatcta
     4561 cgatagcgta tacaacgatc tgattaacta tctggcttca ccggatgaga ccgaaggctt
     4621 tgacgatctg atcaaaaact gccgcgagca acatgaagcg ctgaaagcac agctggaaca
     4681 gggccgtgac cgcctgctgg aaatccactc caacggtggc gaaaaagccc aggcactggc
     4741 agaaagcatt gaagagcagg atgacgatac caacctgatc gccttcgcca tgaacctgtt
     4801 cgatattatc ggtatcaatc aggacgatcg cggcgacaac atgatcgtac tgacgccgtc
     4861 cgatcatatg ctggtgccgg acttccctgg cttgtcggaa gatggcatca ccatcacctt
     4921 tgatcgtgaa gtggcgctgg cgcgtgaaga tgcgcagttt attacctggg aacatccgct
     4981 gatccgcaac ggtctggatc tgatcctttc tggcgatacc ggtagcagca cgatttcact
     5041 gttgaaaaac aaagcgttgc cggtaggtac gctgttggtg gaactgattt acgtggtcga
     5101 agcccaggct ccgaagcagt tgcagctcaa ccgcttcctg ccaccgacgc cggtacgtat
     5161 gctgctggat aaaaacggca acaacctggc ggcgcaggtg gagtttgaaa cctttaaccg
     5221 tcagcttaac gcggttaacc gtcacaccgg cagcaaactg gttaacgccg tgcagcagga
     5281 tgttcacgcg atccttcagc tgggtgaagc acagatcgag aaatctgccc gtgcactgat
     5341 tgatgccgca cgtaacgaag ccgacgaaaa actgtctgcc gagctgtctc gtctggaagc
     5401 tctgcgtgca gtgaacccga acattcgtga cgacgaactg accgccattg agagcaaccg
     5461 tcagcaggta atggaaagcc tggatcaggc aggctggcgt ctggatgccc tgcgtttgat
     5521 cgttgtaacg catcagtaac ggagccgaaa atggggatgg aaaactacaa tccaccgcag
     5581 gaaccctggt tggttatcct gtatcaggat gaccatatta tggtggtcaa caagccgagc
     5641 ggtttgttgt ctgtgccggg tcgtctggaa gagcacaaag acagcgtgat gacgcgcatt
     5701 cagcgtgatt atccgcaggc agaatcggtg catcgtctgg atatggctac cagcggcgtg
     5761 attgtagtgg cgctgaccaa agccgcggag cgggagttaa aacgccagtt ccgcgagcgc
     5821 gagccgaaaa agcagtatgt ggcccgcgtc tgggggcatc catcccctgc ggaaggtctg
     5881 gtagatctgc cgctgatttg cgactggcca aaccgcccga aacagaaagt ctgttacgaa
     5941 acaggtaaac ctgcgcagac ggaatatgaa gtggtggagt atgcggcgga taacacggca
     6001 agagtggtgt taaaaccgat taccgggcgt tcgcatcagt tgcgtgtgca tatgctggcg
     6061 ctgggtcatc cgattctcgg cgatcgtttt tatgcatcac cagaagcgag agcaatggca
     6121 ccgcgtttgt tgctacatgc agagatgttg acgattaccc atccggcata tggcaatagt
     6181 atgacgttta aagcaccagc ggatttttaa tagtcacgct ggcgaagttc gataggccgg
     6241 agaaggcgtt cacgccgcat ccggcaattt tgcgccgatt gcctgatgcg acgcgggccg
     6301 cgtcttatca ggcctacacg ttgaaactta tttaaaccct ttctgctgtt ttatcagctc
     6361 ataggcctgc tgaatttcct gcgctttctg cttcgccatc tccatcatct ctggcggtaa
     6421 acctttcgcc accaacttat cgggatggtg ttcactcatc agcttacggt aggcacgttt
     6481 gatggtggtc gcatcatccg tcggcttcac gcccagcaca ttacaggcat cttccagcgt
     6541 cgggccacgc tgggtttgtt gccagttacc accgccagat tgctgctgat aaccgccgcc
     6601 aaactgcgcc ccgccctgca tcatgcgcaa aaactggtca aactgggcgc gggagatgcc
     6661 taactcttcg gcaatgacat acagcaccgc ccgttcattc gggtgcagtg aaccatcagc
     6721 aaacgccgcc tgaatctgga tctccagaaa catacgaatt aagtcaaaac gaccaaagca
     6781 aacactgcga aactggcgca tcttttcgcg cagcgggtaa ttgtctgatt ttcccacccg
     6841 gaacgcgttt tgcgccgcag tacgggaagc gccatgcagg ttcattcggt ccatcaactg
     6901 gctggcgata tgaatatcag cctccgtgac gcgacctttg gatttggtta aatgccccat
     6961 cacttcaaaa gtggtggcaa aaaacagcgc ctgacgctca cgctggttgg cgaaccacgc
     7021 cattttacgg ctacgggctt tatcaaacat atggccaatt aacaggccta acactacgcc
     7081 ccaaaagccg ccgcccatca gtaaggccac ggccacgcca atgatttttc cccaatactg
     7141 catatattcc ccaaatcgac acacggatat cagggctatc tcccacaata taaaggtgct
     7201 tttaccgttt tccggctgcg gtcaattgtg ggacatcgcc tataatttgc attatcatac
     7261 ctgtcattca atgccgtgcc taacaccact gacgctattc ggacaggatt aacactagcg
     7321 tagagatgac gagtacgtta gtctctgacc gtttgtcacg cgcaacgtta ccgatgatgg
     7381 aacaataaaa tcaacgtatg aaaaaacgta tccccactct cctggccacc atgattgcca
     7441 ccgcccttta tagtcaacag ggactggcag ccgacctcgc ctcacagtgc atgttgggcg
     7501 tgccaagcta tgaccgtcct ctggtacagg gcgataccaa tgacttaccc gtgactatca
     7561 atgctgacca cgcgaaaggg gactacccgg atgacgccgt gtttactggc agcgtggata
     7621 tcatgcaggg taacagccgt ctgcaggccg acgaagtgca gctccatcaa aaagaggcac
     7681 caggacaacc ggagccggta cgtaccgttg atgcgctcgg taatgtccat tacgacgata
     7741 accaggtgat cctcaaaggg ccgaaaggct gggcgaatct gaacaccaaa gataccaacg
     7801 tctgggaggg tgattaccag atggtgggtc gccagggtcg cggtaaagcg gacctgatga
     7861 aacaacgtgg cgaaaaccgc tataccattc tggataacgg tagctttacc tcctgtctac
     7921 cgggttctga cacctggagc gtggtaggta gcgaaattat tcatgaccgc gaagaacaag
     7981 ttgcggagat ctggaacgcc cgctttaagg tgggtccggt accgatcttt tatagcccat
     8041 atttgcagtt gccggtgggt gacaaacgtc gctctggttt cttgatcccg aacgccaagt
     8101 acaccaccac caactacttt gagttctacc tgccgtatta ctggaacatc gcgccaaata
     8161 tggatgccac catcacgccg cattatatgc atcgtcgtgg caacattatg tgggaaaacg
     8221 aattccgtta tctcacccag gcgggcgcag gcttgatgga actggactat ctgccttcgg
     8281 ataaagtcta tgaagatgaa catccagaag atagcaattc acgccgttgg ttgttctact
     8341 ggcaacactc cggagtgatg gatcaggttt ggcgctttaa catcgactac accaaagtca
     8401 gcgacccgac ctatttcaat gattttgata ataaatatgg ttccagtacc gatggctacg
     8461 ccacgcaaaa attcagcgtg ggctatgcgg tgcagaactt tgacgccacg ctttcgacta
     8521 aacaattcca ggtttttgat gataccagtg gcaacagcta tgccgctgaa ccgcagttag
     8581 acgtcaacta ctaccataac gatctcggcc cgtttgatac ccgtgtttat ggtcaggcag
     8641 tgcattttgt taatacaaac agcaatatgc cggaggctac tcgcgttcat ctggagccga
     8701 cgataaacct gccgttatct aacacctggg gcagtatcaa taccgaagcg aagctgctgg
     8761 cgacccatta tcagcagacc aatctcgact ggtataacag caacccacaa aacaataagc
     8821 tggctgattc tgttaaccgc gtaatgccgc aatttaaagt tgacggcaaa atggtcttcg
     8881 aacgtgatat ggaaatgctg gcgccgggtt atacccaaac gctggagccg cgcgcgcaat
     8941 acctgtatgt accgtatcgc gatcagagcg acatctataa ctacgactcc tccttgctgc
     9001 aatccgatta ctcgggcctg ttccgggacc ggacttatgg cggtcttgac cgtatagctt
     9061 ccgcgaacca ggtcacgacc ggtatcacaa ctcgcgtata tgatgatgca gccgttgaac
     9121 gttttaatat ttccgttggt caaatctact atttcacgga gtctcgcact ggcgatgaca
     9181 acataacatg ggagaatgac gacaaaacgg gctcgctggt gtgggcaggc gatacttact
     9241 ggcgtatctc cgag
//
LOCUS       DAMZFT010000183         9245 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.183, whole genome shotgun sequence.
ACCESSION   DAMZFT010000183 DAMZFT010000000
VERSION     DAMZFT010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9245)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9245)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9245
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.183"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(<1..187)
                     /locus_tag="PN977_004582"
     CDS             complement(<1..187)
                     /locus_tag="PN977_004582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001696186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ead/Ea22-like family protein"
                     /protein_id="HDI6044526.1"
                     /translation="MSKIDYQELREAAEKAMHDDWGFDTDLFYELVEPSIVLALLDEC
                     ETLATENAKLKTAHPQLF"
     gene            complement(184..360)
                     /locus_tag="PN977_004583"
     CDS             complement(184..360)
                     /locus_tag="PN977_004583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000753063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6044527.1"
                     /translation="MTDFGSTKYNASFEEWHEVLMDYAGLRGGSAADAEAWRDDYEAG
                     KTPVEAYCDEWGDE"
     gene            complement(612..1190)
                     /locus_tag="PN977_004584"
     CDS             complement(612..1190)
                     /locus_tag="PN977_004584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006859641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2857 domain-containing protein"
                     /protein_id="HDI6044528.1"
                     /translation="MSNNLSQATNGLLMQLVMDLKSGYLRRCEALGLSREEMQMLQGL
                     SIEEIHYLSNSEVSVLRLDINHNNLVRMLQQARTEQKRLQRIDRALALGGSIELMAFY
                     FGLSSVDVAARRRISGIDVRPGRGITLSDDENSELWRLWQKAGINDVESADGLDVMML
                     CAEQMNIPLTAVWHAVRGWHTAGSPEQVRNAS"
     gene            complement(1187..1918)
                     /locus_tag="PN977_004585"
     CDS             complement(1187..1918)
                     /locus_tag="PN977_004585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001696183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2786 domain-containing protein"
                     /protein_id="HDI6044529.1"
                     /translation="MNNDAARQEQLIKRIRKLLALSRNNSNPHEAAQALSRAQALMRK
                     HGITELDADISVIKTSSSKGAPSEAEKVPVWMGRLMNVVAGAFGCHTYLDWRITTGGY
                     WRRIVTFYGFGERPVVAAYAFDVLCRQMQKATKEYLSTQSRKLKHSTRRARAAMFRDG
                     WVYGVRDVVDAFRLTEDETCVMNTWLQNQQFEGMVVFREVKDCHGGDHARWQGYNEGK
                     KATLNHGVDSRDLRPESLISGGGEV"
     gene            complement(1911..3560)
                     /locus_tag="PN977_004586"
     CDS             complement(1911..3560)
                     /locus_tag="PN977_004586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001696182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB family protein"
                     /protein_id="HDI6044530.1"
                     /translation="MSIKRNHSVDLGAAIMQPGRQASTAGNVVALGETPMILTLDQLR
                     PNPDNPRTTRNPRYDDIKSSIFSRGLDTVPKVTRIPESEPDIYIFSDGGNTRYQILKE
                     LWEETGDQRFYRIHVLFKPWPGRLQCVIGHLAENEVRGELTFIEKARGIHKARMIYEE
                     QLSKSVSMRELSSLLTEQGLPVDASSISRMENTLKYLYPWIPELLESGMGSPQVRALL
                     SLRQDAERIWGQFAVATENGGDFDRVFGECCSKFNSPELWSLEMFRDELIGDLLKALP
                     HPVLDYDRWLLELDPRERNRRHHFGEPEPVLIPADSDAMPEPVTHGTSAVTVEKDSGR
                     EQSVISAPADHDTVMNTEQSPVGKAESSVTEQREERRRKEVQPDMYGGEETYSGDYEE
                     DGDTRDAADLPVTSPEEGAQQSSGEIFSEPEFSVGELDDIEYLQNEAFHLAWELASHT
                     GCVDELAMDRESELSAGFGAAEANCSPVMAFLVRLTGEEVLRVNPVSLDALLIGGSTE
                     EEPPLLDDEHAGKLLRLLVVLRRLRGLQRQVEEENVEGRDE"
     gene            complement(3550..4938)
                     /gene="dnaB"
                     /locus_tag="PN977_004587"
     CDS             complement(3550..4938)
                     /gene="dnaB"
                     /locus_tag="PN977_004587"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HDI6044531.1"
                     /translation="MSERYIPRSVDAEQAVLGGLMLDNERWDEVVLMLRGEDFSLAAH
                     RVIFRTMSELATSGQPFDLITLSEHIEKTNNIEALGGFAYLAELSKNTPSAANIIAYA
                     RIVAEKSMLRQLQETGNALITDVAAPDATPRTVLESAERRLFSLSQTGMLHERCEVSL
                     TSAMELALRQLEEGLNAGGMTGTPTGFDELDEMTCGLQPGDLILLAARPSMGKTALGL
                     TFCLNALCNRTDETVFLFSIEMSREQLILRVLSMLSRVELTRLRSSMMDDEEWARVTA
                     TMGRFMNEASFIGDGNRLIIDDTSFQTPSSLRASARRYVRLYGRPSLIMVDYLQLIRS
                     PDQENRTQEIAEISRALKALGKELGCPVLALSQLNRQVEQRADKRPNNGDLRDSGALE
                     QDADLIMFIYRDEVYNSGTLDKGVAEIIVSKQRQGPTGTIRVNFDGRYTLFSPFERGW
                     NFDRAGGNAYVN"
     gene            complement(4931..5116)
                     /locus_tag="PN977_004588"
     CDS             complement(4931..5116)
                     /locus_tag="PN977_004588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001545301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6044532.1"
                     /translation="MSANMLYVPSDRGRQTYRSENVQKVMGLLCPATVRPAPVFVFLH
                     GGFAVKQAPFAREVAYV"
     gene            complement(5430..5924)
                     /locus_tag="PN977_004589"
     CDS             complement(5430..5924)
                     /locus_tag="PN977_004589"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6044533.1"
                     /translation="MNNSSNTTTRNTLSPVESACHALLDFIAREVFENKAPEEDVRER
                     CKVFTALLNEGFKALLDEAGLNLSNLRKQCEELESQITRLDSQSNAICRRGAQIERFI
                     GHHLPFFDLNKVFDTASQSCESAPSPAQSAVSSSQPSNLSEQGTSQRVSSVQPLQGAS
                     DVDE"
     gene            complement(6013..6891)
                     /locus_tag="PN977_004590"
     CDS             complement(6013..6891)
                     /locus_tag="PN977_004590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000698645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HDI6044534.1"
                     /translation="MKILPVVSTKGGEGKSTQSANLAGFLADAGIRTLLIDGDHAQPT
                     ASNIFPLTYEASAGLFELLMRTADLSYPDNIISRTAIDGLDLIVSNDPHEQLKTAMLH
                     APDGRLRLRNVLQHPLFLNNYDVIIVDSQGARSVMLEMIVLSATDSVVGMVNPVLPDV
                     REFIRGTVNVMENLLPYRELGIPLPKVRTLVNCMDYTALARKTLDELAGIIQTGRYSR
                     QLPEGAVSLLSTQIYDLNIYKQGHAAGQPVHRLEKESTRRSDSALVTMHSLACELFPE
                     WKQAFDGLLANGGIRQ"
     gene            6957..7034
                     /locus_tag="PN977_004591"
     tRNA            6957..7034
                     /locus_tag="PN977_004591"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7014..7016,aa:Ser,seq:cga)
     gene            7232..8494
                     /locus_tag="PN977_004592"
     CDS             7232..8494
                     /locus_tag="PN977_004592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase arm-type DNA-binding domain-containing
                     protein"
                     /protein_id="HDI6044535.1"
                     /translation="MALTDAKIRAAKHTDKAYKLTDGAGMFLLVHPNGSRYWRLRYRI
                     LGKEKTLALGVYPEVSLSEARTKRDEARKLISEGIDPCEQKRAKKVVPDLQLSFEHIA
                     RRWHASNKQWAQSHSDKVIKSLETHVFPFIGNRDITTLNTPDLLIPVRAAEAKQIYEI
                     ASRLQQRISAVMRYAVQSGIIRYNPALDMAGALTTVKRQHRPALDLSRLPELLSRIDG
                     YKGQPVTRLAVMLNLLVFIRSSELRYSRWSEIDIDNAMWTIPAEREPLPGVKYSHRGS
                     KMRTPHLVPLSKQAVAILTELQTWAGENGLIFTGAHDPRKPISENTVNKALRVMGYDT
                     TQEVCGHGFRAMACSALIESGLWSRDAVERQMSHQERNGVRAAYIHKAEHLEERRLML
                     QWWADFLGANREQCISPFEYAKINNPLK"
     gene            8745..9245
                     /locus_tag="PN977_004593"
                     /pseudo
     CDS             8745..9245
                     /locus_tag="PN977_004593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001608690.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         2275 a         2577 c         2146 g         2247 t
ORIGIN      
        1 cgaacagttg agggtgtgct gtcttcagct tcgcattctc agttgccagc gtctcgcatt
       61 catccagcag agccagcaca atcgatggtt ctaccagctc atagaaaagg tccgtgtcaa
      121 atccccagtc gtcatgcatt gccttttctg ccgcctcacg cagctcctga tagtcaattt
      181 tgctcattca tcaccccact catcgcaata tgcttcgaca ggcgtttttc cagcttcata
      241 gtcatcgcgc catgcttcgg catcggcggc acttccacca cgtaacccgg catagtccat
      301 taacacttca tgccattctt caaaactggc gttatattta gttgaaccaa aatcagtcat
      361 tttgctctac ctcagtctca tattgttgta tcgctcgtga ctcaacagcg cccaggtttg
      421 tccccggttc ctgctcagta accgccagtc cggaccaaca ttaatcttga tatacccgaa
      481 tcccttttcc ttcagttcat ggtaattcct ctccccgcga cgaaaccggt taacgaggtc
      541 caccgccttc cgctggcagg ctggcggtac cccgggacag ctcagcagac cgggaaccag
      601 ctctttcatc ttcatgacgc attccttacc tgttcagggc ttccggctgt atgccacccc
      661 ctgacagcat gccagacggc cgtgagcggg atattcatct gctctgcaca gagcatcatg
      721 acgtccagcc cgtcagcgct ctccacatcg ttaatccctg ctttctgcca cagtctccag
      781 agttcagagt tttcatcatc gctgagtgtg ataccccgcc ccggacgcac atcaattcct
      841 gaaatacggc gccgtgcagc aacgtccaca ctggaaaggc caaaataaaa cgccatgagc
      901 tcaatcgatc cccccagtgc cagagcgcga tcgattcgct gcaggcgttt ctgttcagtc
      961 ctggcctgct gcagcatgcg tacaaggtta ttgtggttaa tatccagccg cagcactgac
     1021 acctctgaat ttgacagata gtgaatttcc tcaatactca gcccctgcag catctgcatt
     1081 tcttcccggg aaagccccag cgcttcacag cggcgcagat agccactttt gagatccatc
     1141 accagttgca tcaacagtcc gttggtggcc tgtgacaagt tattactcat acctcacctc
     1201 cgcccgatat caggctttcc ggtctcagat cccgggaatc aacaccgtga ttcagcgttg
     1261 cctttttccc ctcgttgtat ccctgccagc gcgcatggtc accgccatgg cagtccttta
     1321 cctcccggaa gacgaccatt ccctcaaatt gctggttctg cagccacgta ttcattacac
     1381 acgtctcatc ttctgtgagc cggaatgcat ccaccacatc acgcacccca taaacccagc
     1441 cgtcacgaaa catcgcagct cttgcacgac gggttgagtg ttttaatttc cggctctggg
     1501 ttgagagata ctcttttgtc gccttttgca tctgccggca gagaacatca aaggcatacg
     1561 ccgccacaac cggacgctcc ccaaaaccat aaaatgtgac aatccgccgc cagtatcctc
     1621 cggttgtgat acgccaatcc aggtatgtat gacatccgaa tgcccctgca acaacattca
     1681 taaggcgccc catccatacc ggaacttttt ccgcttctga cggcgctccc tttgatgatg
     1741 aggtttttat caccgaaata tcagcatcca gttcggtgat accatgcttc cgcatcagcg
     1801 cctgtgcgcg ggaaagagcc tgagctgcct catggggatt actgttattc cggctcagcg
     1861 ccagcagttt tcttatccgt ttaatcagtt gctcctgacg tgctgcatca ttattcatca
     1921 cgcccctcca cgttctcttc ttccacctgg cgctgcagcc cccgcagacg acgcaacacc
     1981 acaagcagac ggagtaactt gccggcatgc tcatcatcca gcagtggtgg ctcttcttca
     2041 gtactgccac caatcagcag tgcatcaaga gaaaccggat taacccgcag aacctcctca
     2101 ccggtcagcc tgacaagaaa tgccatgacc ggagaacaat tcgcctctgc cgcgccgaat
     2161 cccgcagaca gttcgctttc cctgtccata gccagttcat cgacacagcc tgtgtgtgac
     2221 gccagctccc atgccaggtg aaatgcctca ttctgcagat attcgatatc atccagttca
     2281 ccaacactga attccggctc actgaaaatt tcaccgctgc tctgctgagc accctcttcc
     2341 ggtgacgtta ccggcagatc cgcagcatca cgggtatcgc catcctcttc gtaatcgccg
     2401 ctgtatgttt cttcaccacc atacatatcc ggctggactt cctttctgcg gcgttcttcg
     2461 cgttgttcag taactgaact ctccgccttt ccaacagggg attgctccgt attcatgact
     2521 gtgtcatggt cagccggtgc ggaaatcaca gactgctccc ttccgctgtc cttctccacc
     2581 gtcactgccg acgtaccgtg agtcacaggc tctggcatcg catcactgtc tgcgggaatg
     2641 agcaccggtt caggttcacc aaaatgatga cgtcggttac gttcccgcgg atccagttca
     2701 agcagccagc ggtcataatc cagcaccgga tgtggtaacg ctttcagcag atcaccaatc
     2761 agctcatccc ggaacatctc cagtgaccag agttccggtg aattgaattt gctgcagcat
     2821 tcaccaaaca cgcggtcaaa atcccccccg ttctctgttg ccaccgcaaa ctgtccccag
     2881 atacgttccg catcctgccg gagagacagt aaagccctga cctgaggact ccccattcct
     2941 gattccagaa gttcggggat ccacggatac aggtatttca gggtgttctc catacggctg
     3001 atactggatg catccaccgg taatccctgc tcagtcaaaa gggatgacaa ctctcgcatg
     3061 gacacacttt tgcttaactg ctcttcataa atcatccgtg ctttatggat accccgggcc
     3121 ttttcaataa acgtcagttc gcccctgact tcattctccg ccaggtgacc aatgacgcac
     3181 tgaagccgtc ccggccatgg cttgaaaagc acatgaatac ggtagaaacg ctgatcaccg
     3241 gtctcttccc acagctcttt caggatctga tatcgcgtgt taccgccatc gctgaaaata
     3301 tagatatccg gctcactctc aggtatccgg gtgactttgg gcaccgtatc cagtccacgg
     3361 gagaaaattg aacttttaat atcgtcgtac cgtggattac gcgtggtcct cgggttatcc
     3421 gggttggggc gcagttgatc cagcgtcaga atcatcggtg tttcccccag cgccaccacg
     3481 ttacctgccg tgctggcctg acggcccggt tgcatgatgg cagcccccag atcaacggaa
     3541 tgatttcgtt taattgacat acgcatttcc tccggcacgg tcaaaattcc atccacgttc
     3601 aaacggtgaa aacagggtgt agcggccgtc gaagttcacc cgtatcgttc cggttggtcc
     3661 ctgccgctgt ttgctcacaa tgatttcagc aacgccctta tccagcgtgc cggagttata
     3721 aacctcgtca cggtaaataa acataatgag gtcagcatcc tgctccagcg ccccggaatc
     3781 acgcaggtca ccgttattgg ggcgtttatc cgcccgctgc tccacctgac ggttaagctg
     3841 agacagtgcc agtaccgggc atcccagctc tttacctaac gccttaagcg cgcgggatat
     3901 ttccgcaatt tcctgcgttc ggttctcctg gtccggagaa cgaatcagct ggaggtaatc
     3961 aaccattatc agggaaggtc tgccatacag acggacataa cgacgggcgc tggcacgcag
     4021 cgatgacggc gtctggaagg aggtgtcatc aattatcagg cggtttccat ctccgatgaa
     4081 tgacgcttca ttcatgaagc gtcccatggt tgccgtgact ctcgcccact cttcatcatc
     4141 catcatgctg ctgcgaagac gtgtcagctc caccctggag agcattgaca ggacacgcag
     4201 aatcagctgc tcccgggaca tttcgatact gaaaagaaat accgtttcat cggtccggtt
     4261 acacagggca ttaagacaga aggtaaggcc cagcgctgtt ttccccatag acgggcgtgc
     4321 agccagaaga atgaggtcgc cgggctgcag accacaggtc atctcatcca gttcatcaaa
     4381 tcccgtgggc gtgcccgtca tcccgccggc gttcagtccc tcttccagtt gacgcagcgc
     4441 cagctccata gccgaggtga gactgacttc acatcgctca tgcaacatgc cagtctgtga
     4501 aagtgaaaag agccgacgtt cagcggattc aagaacagtg cgtggtgtcg catccggtgc
     4561 ggccacatcc gtaatcagcg cattccccgt ctcctgcaac tgacgcagca tgctcttttc
     4621 agcaacaata cgggcataag cgataatatt ggctgcagaa ggcgtatttt tgctgagttc
     4681 agccagataa gcgaaaccac ccagtgcctc aatattgttt gttttctcaa tatgctcact
     4741 cagcgtgatg aggtcgaacg gctgaccgct ggttgccagc tctgacatgg tgcggaatat
     4801 cacccggtgt gcggccagag aaaaatcctc cccccggagc atcagtacca cctcatccca
     4861 gcgttcatta tcgagcatca ggccgcccag cacggcctgc tctgcatcaa cggagcgtgg
     4921 aatatagcgt tcagacatac gccacctccc gggcaaacgg agcctgtttc acagcaaacc
     4981 caccatgcaa aaaaacgaaa acgggtgccg gacgaacagt tgcagggcat aaaaggccca
     5041 taactttctg tacattttcc gaccggtaag tttgccgacc ccggtcagaa ggtacgtaca
     5101 gcatatttgc gctcataatc aacatgttta cgaagagaaa taccttatga acacaaatca
     5161 tcagcaactg ccgtccggca ttgataacaa ccatgatgag gatggcaaca tggcaaaata
     5221 ttatgaaaac tggaaagtcg gaggtatgag caccgacgat gtggaacggt gcaaaacctt
     5281 ctttgaccag ggaacagaaa tctttcccgg agctgaccgg aagctcgctt accgactttg
     5341 ctggctcgga cactggctga ttaacagccg ccgctggttt tttctggacg ataacagtat
     5401 cagcgttcaa caagccgtta agtttgctct tattcatcaa catcactggc tccctgcaac
     5461 ggttgaacag atgacacccg ctgagatgtc ccttgctctg acagattact gggctgacta
     5521 ctggacacag ccgactgagc cggagaaggc gctgattcac atgactgact ggcagtatca
     5581 aaaactttgt tcaaatcgaa aaaaggtaaa tggtgaccaa tgaagcgttc aatttgagca
     5641 cctctgcgac aaatagcatt ggactgagaa tcaagtctgg ttatctgaga ttccagttcc
     5701 tcacactgtt tacgaaggtt tgacagattc agccctgcct catcaagcag ggctttaaat
     5761 ccttcgttaa gcagcgctgt aaatactttg caacgttccc tgacgtcctc ttccggtgct
     5821 ttattctcaa aaacttcccg ggcaataaaa tccagtaagg catgacaggc tgactcaacg
     5881 ggtgaaagcg tattacgggt tgtggtattg ctgctgttat tcatagggtt aatccttcac
     5941 gtcatattgt ccggtattga taacgtccat aagccagggt aacggagctg catggttctc
     6001 gcttaacctg aatcactgac gtattccccc gttagccagt agcccgtcaa acgcctgttt
     6061 ccactcagga aatagttcac aggccaggct gtgcatcgtg acaagcgcgg aatcactgcg
     6121 acgtgtggat tctttttcga gacgatgtac gggttgacca gccgcatgcc cctgcttgta
     6181 aatattcagg tcgtaaatct gtgtggaaag aagggaaacc gcaccttcag gtaactgcct
     6241 gctgtaacga ccggtctgaa taattccggc cagctcatcc agcgtcttcc tggccagtgc
     6301 cgtataatcc atgcagttaa ccagcgtgcg tactttcggc aggggtatcc ccagttcccg
     6361 gtaaggcagc aggttctcca taacgttgac agtgccgcga ataaactcgc gcacatcagg
     6421 gagtaccgga ttgaccatcc cgacaaccga atcagtggcc gacagcacaa tcatttcgag
     6481 catgacagaa cgcgcaccct gagaatcaac tataatgaca tcgtagttgt tcagaaaaag
     6541 cggatgctgc aggacattcc ggaggcgcag acgaccatcc ggggcgtgaa gcatcgcagt
     6601 ttttaactgc tcatgagggt cattggagac tatcagatcg agtccgtcga tagctgtgcg
     6661 ggaaatgatg ttatcgggat aactgaggtc agccgtgcgc atcagcaact caaaaagacc
     6721 ggctgacgct tcatatgtca gcggaaagat gttgcttgcc gtgggctgag catgatctcc
     6781 gtcaatcagc agggtacgga tacctgcatc ggcgagaaaa ccagccaggt tagcggattg
     6841 tgttgattta ccttcgccac cttttgttga gacaacggga agtattttca tatctgcgtc
     6901 ctatcgacag ttatgtgatc ggatgcgcat gaaagacttc gacaacaact tgacatgccc
     6961 gtaactatct gaattttatg acatggggat tgaaaatccc cgtgtccttg gttcgattcc
     7021 gagtccgggc accaaattca tatcaacgga cctccacgga ggtccgtttt tcgtttcaga
     7081 acgccacaat ttaagcgttc tgcctccaaa tcaattctac cgaactcaac cagattcccc
     7141 ccacatcaac cccattgtgt gggtataatt gcgggtatac cccagttcga cagaatttgt
     7201 accctcttta acgcattcag aaggctgaac tatggcactg actgacgcaa aaatccgggc
     7261 tgcaaagcac actgacaagg cttataaact cactgacggg gctggcatgt tcctgctggt
     7321 tcatcctaat ggttcccgtt actggcgtct ccgttatcgt attctgggta aggagaagac
     7381 tctggcactt ggtgtatatc cagaagtttc tctctccgaa gctcgtacaa aacgggatga
     7441 ggcccgaaaa ctgatttcag aggggattga tccttgcgaa caaaaaagag ctaaaaaagt
     7501 agtccctgat ttacaactct cttttgaaca tattgcacga cgctggcatg ccagtaataa
     7561 acaatgggca caatcacaca gcgataaagt aatcaaaagc ctcgagacac acgttttccc
     7621 ctttatcggc aaccgggata ttacaacact caataccccg gacctgctta tccctgttcg
     7681 tgctgcagaa gcaaaacaaa tttatgaaat cgccagtcgt ctgcagcaaa gaatatctgc
     7741 tgtaatgcgt tatgccgtac agtctggcat catcagatat aacccggctc tggatatggc
     7801 tggtgcattg accacggtaa aacgccagca tcgccctgct ctggatcttt cacgcctgcc
     7861 tgaacttctg tcgcgtattg acggttataa aggtcagcct gtcacccgac tggcagttat
     7921 gctgaattta ctggttttta ttcgttccag tgaactcaga tattcccgct ggtctgaaat
     7981 tgatattgac aatgccatgt ggactattcc agccgaacgc gaacctctgc caggcgtaaa
     8041 atactcacac cggggctcca agatgcgaac accacatctt gtgccactca gcaaacaggc
     8101 tgtagccata ctgacagaac tacagacatg ggctggtgaa aatggtctga tctttacggg
     8161 agcacatgac ccgcgtaaac cgatcagtga aaatactgta aataaagccc tgagggtgat
     8221 gggatatgac acaacccagg aagtctgtgg tcatggattc cgggcgatgg cgtgcagtgc
     8281 attgattgaa tcaggtttgt ggtcccgcga tgctgtggaa cgtcagatga gccatcagga
     8341 gcgtaatggt gtacgtgctg catatatcca taaagcagaa catctggaag aacgtcgact
     8401 gatgctacag tggtgggcag attttctggg tgcgaacaga gaacaatgta tcagcccgtt
     8461 tgaatatgca aagattaaca atccattaaa atagtaatca tcccgggctg ccagcccggg
     8521 aattatttta tgaatatttt tctttgtgag taactgggaa aaggatagcg aagtaaatta
     8581 ctttaatgaa ttattccagg ggctcattaa agtcctttcg taatacttta tagtcaaaca
     8641 cgttatggct ggcatcaaaa actacggatt tcccctgtat caacgccacg atttttgacc
     8701 gttaggggat gcggacgatt tcctggactc tcaggagatg aaacatgttt actgaagaac
     8761 aaaaaattcg caccattgag ctttatttca aatatggtaa gaagctggcc ccgtcgtacg
     8821 cgaactgggc tacccttcga aaagaaatct gcggcgttgg atccgttcat gggaagcagg
     8881 tggcggatca aaagaatcca ttcgtcataa gcaccgttac tccgatgagc aaaaacaggt
     8941 tgccgttgaa cattatctta accacggctg ctgcctggcg tttaccagca ggactctggg
     9001 ttatccctgt accgatatgc tcgctcgctg ggttaatgaa cgttcccccg acagacgacg
     9061 tgtatttacc agtaagacca atccggttgc gtcctttgaa ccagaagtca aacgtcaggc
     9121 tgtcatggta ctctgtacac gacaaggatc agccagtgaa atcgccaggc gtattggtgt
     9181 cagtcgcgcg gtattgtata aatggaaaga tgaaattatc ggcaacagtg cttaccagac
     9241 tatgc
//
LOCUS       DAMZFT010000184         9180 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.184, whole genome shotgun sequence.
ACCESSION   DAMZFT010000184 DAMZFT010000000
VERSION     DAMZFT010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9180)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9180)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9180
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.184"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..1648
                     /locus_tag="PN977_004594"
     CDS             <1..1648
                     /locus_tag="PN977_004594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1998 domain-containing protein"
                     /protein_id="HDI6044536.1"
                     /translation="LRGGSNDRQGSNKLCHGHVHKDLWLGYSSRTDMVELQLNDDNGL
                     LIRDEVAARSLAVALREGLAHKLGIENTELGVTTQQARDINGYTGYSIFIYDNNAGGA
                     GYAVQLIDHWADVFNYARKLLDCSCDKFCHHCLLSYDSQHYVNRLDRHHALTLLTNVR
                     LQRLNLAPEYQYFGDGSRVETNPLSLRIAQCLNSEIYDSCSLVLAGPQEQWDFAQWPL
                     FKELLQFASSGGHVELLVATPLANLTDSSRHQLSALAAMPGGRLQVKSIATAQLMQGK
                     GRWLAQVTREGQSQQWAADDSATVAPGELWGQSASSPVVTLKGTSGKTFLGQILSAED
                     LLPALPTGAVRINLCEQLDGPLEGFGSRFWSLVTQQHAGWKQAFTRHKEITHVEYSDR
                     YLNSPFTARLLGEILTELVEQGMAERASLTVCVKKLDYNSRQHDALYNAWLNEEDRQQ
                     VVTTLLEEGYLGPAWLGAISWLTGDNQSTEHGRELTVTFSDGSQHYVLLDMGLSYWRC
                     IEDTFFDFALRVPQQVERLANTRARAVAPGNDLRSYIIAG"
     gene            1872..4730
                     /locus_tag="PN977_004595"
     CDS             1872..4730
                     /locus_tag="PN977_004595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313288.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HDI6044537.1"
                     /translation="MNKQNYAPGMRVVIRDAEWRIRRADDSGDGGYLLTCDGISELVR
                     GKEGLFLTKLEQKVEILDPAKTHLVEDESANYQAAQLYIESQLRQRVPTDSKVHFGHL
                     AAMDSMPFQLDPTRMALAQPRQRILIADAVGLGKTLEAGILVSELIRRGRGKRILVLA
                     VKSMLTQFQKEFWSRFAIPLTRLDSAGLQKVRNRIPTNHNPFHYFDKTIISIDTLKQD
                     IEYRHHLENAWWDIIVIDEAHNVAERGTSSLRSKLAKLLAGRSDTLIMLSATPHDGKA
                     ESFASLMNMLDPTAIANPKEYEYADFADKNLVVRRFKKDVKDQMSGEFPERNIVKLTR
                     LASGAEEEAYRRLVESQFRDDDDEQAQSNKGRLFKITLEKALFSSPMACASVVANRLK
                     RLESRKDHNSQSQINELESLLLALNNIDASQFSKYQLLLDTIRKDLAWKANNTEDRLV
                     IFTESIKTLEFLEQQLRADLKLKDDQIATLRGDQGDTVLMETVEAFGKTQSPLRLLVC
                     SDVASEGINLHHLSHKMIHFDIPWSLMVFQQRNGRIDRYGQKHQPQIRYLLTEASEPQ
                     INGDMRVLEVLINKDEQAQKNIGDSSEFTGKFTQEEEEEQVAEFMMQDDGASLFDQLL
                     NSNVSESAEHDLFGEICSAVSSDASMVTETDTSLFASEQAYCERALGYLKASGQTIQY
                     ETLPDNTLSLVAPEELRRRFNQLPPEIAPENWQLYLSQDKTVITDAIARARGEQHAWP
                     DVQYLWQINPVVQWLDDKISSAFGRHQAPVIRLPYLLEPDEDHFILSGLFPNRKSHPM
                     VNPWIVVSFNRESLIGSQPFAEFLQRHPQLSNKLTNSGGKDRNHQRQQDLLEAAIAHA
                     REVFIHDRNAFETHINQQLNEHLQKLDVLRGRQLSQLELDFADNKQQLSVKQSRKEQR
                     QREIEHNFDSYIEWIEDTMTTEKEPYIQVIAVITGAEG"
     gene            4733..>9180
                     /locus_tag="PN977_004596"
     CDS             4733..>9180
                     /locus_tag="PN977_004596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent DNA methyltransferase"
                     /protein_id="HDI6044538.1"
                     /translation="MALVGINNENEFYSNHYLGEVFTSDIRDVLEPWIAQENAAREAE
                     RAAREQGKDVEPGYRAPWNQFNSLATEFFRKLAEHEKQRQIPQRLADQRNRWQPLLKA
                     LGYEITPQIQMLDDDTPLPVLARYNSTDGSPWLWIVEAHDQEEGTLDPLALSLLTAQF
                     PADTDKHKRDSLRKKANGEYRSWQDLLSTAVFTQNEPPRFVLLLGNRQLLLLDRTKWA
                     QNRLLRFDFEEILSRRETDTLKATAVLLHKDSLLPGSGAPYLDSLDDNSHKHAFGVSE
                     DLKYALRESIELLGNEAMHYLIDRGLANYTGNRAVDPDELSRECLRYMYRLLFLFYIE
                     ARPELGYAPMTAKTYLQGYSLETLRDLEMIPLTSEEDRNGRYFHDSLNMLFKLVREGY
                     NGGVKMQSDLESGDRITIHSHQFSVPRLESHLFDANNTRILNRVVFRNETLQQIIQAM
                     SLSRPGKGRFNRRGRISYRQLGINQLGAVYEALLSYRGFFASEDLYEVKKAGEEFNEL
                     ETGYFVSKDEIGKYHEDEKVYEKDGSLRIHRKGSFIYRMAGRDREKSASYYTPEVLTR
                     SLVKYALKELFKEQIDPITDLHAKADAILNLTVCEPAMGSAAFLNEAINQLAEAYLFH
                     KQQAEGRRIPQDRYTQELQRVKMYIADNNVFGVDLNPVAVELAEVSLWLNAISGDAFV
                     PWFGYQLHCGNSLVGARRQVFNKSELTYKKAKDPSWLNSEPVELAMNTPREETQIFHF
                     LLPDGGMANYSDKTVKQRYPDDFKALDSWRKEFIKSFAGHEIADVQRISEKVEALWNT
                     YRQQLKAERLKTADSYPVWPAENSEQTRSSLSSKDETFSGRLEDNSAYQKLRWVMDYW
                     CALWFWPIDKADELPDRGTWLFEIETLLDGIVITEKVTEVAEHTTGDLFAEEGLLREE
                     SSLFSGAGRLKTEVLFRHLPRLAIVDALRKQYRFFHWDLEFCDLFAERGGFDLMLGNP
                     PWLKVVWEERGILGDYKPELVLHNLSAANISSLREESFARVYGLEDAWRSEFESAVGL
                     QNYLNSQQNFSCLLGQKTNLYKCFLPVSWRLISEHGVSGLLHPDGVYDDSKAGELRAK
                     IYCRLRDHYQFQNELGLFPDVHHATTFSINIYGPLQESPSFTHMSNLFSVYTIDSSIL
                     HDNQGEIPGIKEESYNGDKLKINWNISPHKSRVLTITIDELRLFAGIFDDNSTPALHA
                     RLPALHTVQLLEVLRKFDVKSKKLSSIKNNYYSTVMFDETFAQRDGTTKRKTEFPSGI
                     SQWILSGPHFFVGNAYYKTPRTLCKLNSDYDSIDLMTIPDNYMPRVNYIPNCEYSDFL
                     NRIPTVSWDGNVFPVTNGYRFVNREMIGSTSERTFIATIIPPGISHINTCLSTIFKHD
                     YDLLDFFSMSLSIVVDYRVKCTGMGHANQSLVNQLPLLSNEKFRASLHARSMALVSIT
                     EHYKKLWCSIYSSEFKIQYWSRDLPQLPQDFFTNLTPEWQRNCALRS"
BASE COUNT         2399 a         2143 c         2378 g         2260 t
ORIGIN      
        1 gttgcgaggg ggcagtaacg atcgtcaggg gagcaataaa ttgtgccacg gtcatgtgca
       61 taaagatctg tggttggggt atagcagtcg tacagatatg gttgagctgc aactcaatga
      121 tgataatggc ctgttgatcc gtgatgaggt tgccgcgcga tcccttgcgg tcgctttgcg
      181 tgaagggtta gcgcataaat tgggtatcga aaatactgag ctgggcgtga cgacgcagca
      241 agcgcgcgat atcaatggct ataccggata ttccatcttc atttacgaca ataatgcggg
      301 tggtgcaggt tatgcagtgc aattgatcga ccattgggct gatgtcttta attacgccag
      361 aaaattgctc gattgcagtt gcgataaatt ctgtcaccac tgcttgctca gctacgacag
      421 tcagcattat gtcaacagat tagatcgtca ccatgcatta accctgctta ccaatgtacg
      481 gttgcagcga cttaacctcg cgccagaata tcagtacttc ggcgatggta gccgggttga
      541 aactaacccg ctgagtttac gtattgcgca atgcctgaac agcgagattt atgactcctg
      601 ctcgctggtt cttgcggggc cgcaggaaca gtgggatttt gcccaatggc cattatttaa
      661 agagttgctc cagtttgcct cctccggtgg gcatgttgag ttgctggtgg ccacgccgtt
      721 ggcaaactta actgatagct ccagacatca gctttccgcg ttggcggcga tgccgggagg
      781 acgcttacaa gtgaaatcca ttgccactgc gcaactcatg caaggaaagg gacgctggct
      841 tgcgcaggtg actcgcgaag ggcaaagcca acagtgggcc gcagacgaca gcgctaccgt
      901 tgcacctggt gaattatggg gacaaagtgc gtcttcacca gtagtgacac ttaaaggaac
      961 gtccggaaaa accttcttgg gtcagatctt aagtgcagaa gatttactgc ccgccttacc
     1021 caccggggcg gtgcgcatca atctctgcga acaactggac gggccgctgg aaggttttgg
     1081 ttcgcgtttc tggtcgttag tcacgcaaca gcacgctggc tggaagcagg catttacccg
     1141 ccacaaagag ataacgcatg ttgagtacag cgatcgttat cttaatagcc cattcacggc
     1201 gcgtttgtta ggtgaaatct taactgagct ggttgaacag ggaatggcgg aacgcgccag
     1261 cctcactgtt tgcgtcaaaa aattggatta caactctcgc caacacgacg ctctctataa
     1321 cgcgtggctt aatgaggaag atcgtcagca ggttgtgacc actttacttg aagaaggata
     1381 tctggggcca gcgtggctgg gagcaattag ctggttaacg ggggataacc agagtacgga
     1441 gcatggacga gaactgacag tcaccttcag cgatggcagt cagcattatg tcctgctcga
     1501 catggggctt agctactggc gctgtattga agacaccttc tttgattttg ctctacgagt
     1561 accgcagcaa gttgaacggc tggcaaacac gcgagcgcga gctgttgccc cgggtaatga
     1621 ccttaggagt tatattatcg ctggataaat aattgctccc ctgaggatat cttccagggg
     1681 agttgaccca gctcactttt taataatttt tgtttgaaaa tcatgcgcaa attatgcgca
     1741 ttccctccca ttttgtcact ttgccggggg ccttcatgat atctttaagg ccctcttttt
     1801 tatcgcagtg tgccgcgcca catttttcag gatgacgtta ccgccactgc cccaaataga
     1861 cagaaaaacc gatgaataag caaaattatg cacctggtat gcgggtggtt attcgtgatg
     1921 ccgaatggcg tattcgccgg gcggatgaca gtggtgatgg tgggtatttg ctgacctgcg
     1981 atggtatttc agagcttgtg cgcggtaaag aagggttatt tctgaccaaa ctggaacaaa
     2041 aagtagagat cctggatccc gcaaaaacgc atttggtgga agatgaatcc gccaattatc
     2101 aggcggcgca gttgtatatc gaaagtcagt tacgccaacg cgtaccgaca gacagtaaag
     2161 tccattttgg tcatctggcg gcgatggatt ccatgccgtt tcagctcgac cctacgcgga
     2221 tggctctggc acagccgcgc cagcggatac tcattgccga cgctgtcggt ctgggtaaaa
     2281 cgctggaagc cggtattctg gtttcggagc tgatccgtcg tgggcgcgga aaacgtattc
     2341 tggttctggc ggttaaatcg atgctgacac aattccagaa ggagttctgg agccgttttg
     2401 cgatcccatt gacccgcctg gattctgctg gtttacaaaa ggtgcgtaac cgcatcccta
     2461 ccaaccacaa cccgtttcac tattttgaca agaccattat ctccatcgat acgctgaaac
     2521 aagacattga atatcgtcac catctggaaa atgcctggtg ggatattatt gtcattgatg
     2581 aggcccataa cgtcgcagaa cgtggaacca gttcgttacg cagtaaactg gcaaaactgc
     2641 tcgccgggcg tagcgatacg ctgattatgc tttcagcgac gccgcatgat ggtaaggctg
     2701 aaagttttgc cagcctgatg aacatgcttg acccgacggc gatcgccaac ccgaaagagt
     2761 atgaatacgc cgactttgcc gataaaaatc tggttgtccg tcgcttcaaa aaagacgtga
     2821 aagatcagat gtccggcgag ttccccgagc gcaatattgt caaactgact cgcctggcat
     2881 ccggtgccga agaagaagca taccgtcgcc tggttgagag tcagttccgt gatgatgacg
     2941 atgaacaggc ccagtctaac aaaggtcgtc tgttcaagat aaccctcgaa aaagcgctat
     3001 tttccagccc gatggcgtgt gccagcgtag tggctaatcg tctgaagcgt cttgagagcc
     3061 gtaaagatca taatagccag agccagatca acgaactgga gtcattgctg ttggcgctga
     3121 ataacattga tgccagccag tttagcaaat accagttgct gctcgacacc atccgaaaag
     3181 acctcgcctg gaaagccaat aacacggaag atcgcctggt gatcttcact gaaagcatta
     3241 aaacgctgga gttcctcgaa cagcaactgc gagcggatct gaaattgaaa gatgatcaga
     3301 tcgccacgct gcgcggcgat cagggcgata cggtgttgat ggaaaccgta gaagcctttg
     3361 gtaaaacgca gtcgccgttg cgtcttctgg tttgttcgga tgtggcatct gaagggatca
     3421 acctgcatca ccttagccac aaaatgattc actttgatat tccgtggtcg ttgatggtat
     3481 tccagcaacg taacgggcgt attgaccgct atgggcaaaa acatcagcct caaatccgct
     3541 atctgcttac cgaggccagc gaaccgcaga tcaatggcga tatgcgcgta ctggaagtgt
     3601 tgatcaacaa agacgaacag gcgcagaaga acattgggga cagttcggag tttaccggca
     3661 aatttaccca ggaagaggaa gaagagcagg ttgcagagtt catgatgcag gatgatggtg
     3721 ccagcctgtt tgatcaactg ctgaacagca acgtctcgga aagcgccgaa catgatctgt
     3781 ttggcgaaat atgcagtgcg gtttccagcg atgcctcaat ggtcaccgaa acagacacca
     3841 gcttgtttgc cagcgaacag gcgtactgcg aaagagcatt gggttacctg aaagccagtg
     3901 gtcaaactat ccagtatgaa accttgcctg ataatacctt gtcgctggtg gcgccggagg
     3961 agttacgccg ccgcttcaat cagctaccgc ctgaaatcgc gccggagaac tggcagctct
     4021 atttaagtca ggataaaacc gtcatcaccg acgcgattgc acgcgctcgc ggtgagcaac
     4081 atgcctggcc cgatgtgcag tatctctggc aaatcaaccc ggtagtgcag tggctggacg
     4141 ataaaatctc ttcggctttt ggtcgtcatc aggcaccggt tatccgtctg ccatacctgc
     4201 ttgaacccga tgaagatcac ttcattcttt ccgggttatt cccgaaccgt aaatcacatc
     4261 cgatggtgaa cccgtggata gtggtgagct ttaaccgtga atcgctaatc ggcagtcagc
     4321 catttgctga gtttttacaa cgtcatccgc agttgagtaa caagctgact aacagcggcg
     4381 gtaaagatcg taatcaccag cgccagcagg atttactgga agcggctatt gctcatgccc
     4441 gggaggtatt catccatgat cggaatgcgt ttgaaacgca cattaaccag caactgaatg
     4501 agcatctgca aaagctggac gttttgcgtg ggcggcagtt gagccaactt gagctggatt
     4561 ttgccgataa caaacagcaa ttgtcagtta agcagagccg taaagagcag agacaacgcg
     4621 aaatcgaaca caattttgac agctatatcg aatggattga ggacaccatg acgactgaaa
     4681 aagaacccta cattcaggta attgctgtta tcaccggagc ggagggttaa tcatggcgct
     4741 ggtcggtatt aataacgaaa acgaatttta ctctaaccac tatttgggtg aggtattcac
     4801 cagtgatatc cgcgatgtgc tggaaccctg gatagcccag gaaaatgcag cgcgtgaagc
     4861 ggagcgtgcc gctcgtgaac agggcaaaga cgtagagccg ggataccgcg ctccgtggaa
     4921 ccagtttaac agtctggcga ctgagttttt ccgcaaactt gccgagcacg aaaaacagcg
     4981 tcagatcccg cagcgtctgg ccgatcaacg taatcgctgg cagccattgt taaaggcgct
     5041 gggctacgaa attacgccgc agatccaaat gctggatgac gatacaccac tgccagtact
     5101 ggcgcgttac aacagcactg acggtagccc gtggctgtgg attgttgaag cacacgatca
     5161 ggaagaagga acgctggatc cgctggcgct ctccttactg accgcgcaat tcccggcgga
     5221 taccgacaaa cataagcgcg acagcctgcg caaaaaagcc aacggtgaat atcgcagctg
     5281 gcaggatctg ctctctacgg cggtcttcac ccaaaatgaa ccgccgcgtt ttgtgctgct
     5341 gctcggtaac cgtcagctat tgttgttgga ccgtactaag tgggcgcaaa accgtctgct
     5401 acgttttgat tttgaagaga ttttaagtcg tcgtgaaacg gatacgctga aagcgacggc
     5461 agtgttgcta cataaagatt cgctgctgcc gggcagtggg gcaccatatc ttgactcgct
     5521 ggatgacaat tcgcacaaac atgcgtttgg tgtttcggaa gatctgaaat atgccctgcg
     5581 cgaaagcata gagttgttgg gcaacgaagc gatgcattat ctgatcgacc gtggcctggc
     5641 aaactatacc ggtaaccgtg cggtggaccc ggatgaactg agccgcgaat gtctgcgtta
     5701 catgtaccgc ctgctgttcc tgttctacat tgaagcgcgc ccggagctgg gttatgcgcc
     5761 aatgacagcc aaaacctatc tgcaaggtta cagcctggaa acgttgcgcg atctggagat
     5821 gatcccgctg accagcgaag aagatcgcaa cgggcgctac ttccacgaca gcctgaatat
     5881 gctgtttaaa ctggtgcgcg aaggctacaa cggcggcgtg aaaatgcaga gtgacctgga
     5941 gagcggcgac cggatcacca tccatagtca tcagttcagc gtcccgcgtc tggaaagtca
     6001 tctgtttgat gccaacaaca cgcgcattct taaccgcgtg gtattccgta acgaaaccct
     6061 gcaacagatt atccaggcga tgtcgttaag ccgcccgggc aaagggcgct ttaaccgccg
     6121 cggacgtatt tcttatcgcc agttgggtat caaccagttg ggtgcggtgt atgaggcgct
     6181 gctctcctat cgcggattct tcgccagcga agatctctac gaggtgaaga aagccgggga
     6241 agagtttaac gagctggaga cgggttactt cgtcagtaag gatgagattg gcaaatacca
     6301 cgaagacgag aaggtctacg agaaagacgg cagtctgcgc attcaccgca aaggcagttt
     6361 tatctaccgt atggccgggc gcgaccgtga gaaatctgcc tcttattaca ccccggaagt
     6421 gctgacccgc tcactggtta aatatgccct gaaagaactg tttaaagagc aaattgatcc
     6481 gattaccgat ctgcacgcta aagctgatgc catcttaaac ctcaccgtgt gcgaaccggc
     6541 gatgggcagc gcggcgttcc ttaacgaagc catcaaccag ctggcggaag cgtatctgtt
     6601 ccacaagcag caggcggaag gtcgccgtat tccgcaggat cgttacaccc aggagttaca
     6661 gcgggtgaaa atgtacattg ccgacaacaa cgttttcggc gtggacttaa acccggtggc
     6721 ggtggaactg gcggaagtgt cgctgtggct gaacgccatc agtggcgatg cctttgtacc
     6781 gtggtttggt taccagctgc actgcggtaa ctcgctggtg ggcgcgcgcc gtcaggtgtt
     6841 caacaagagt gaactgacct acaaaaaagc caaagatccg agctggctta acagcgagcc
     6901 ggtcgaactg gcgatgaaca cgccgcgtga agagacgcag atttttcact tcctgctgcc
     6961 cgacggcggt atggctaact acagcgataa aaccgttaag cagcgttatc cggatgactt
     7021 caaagccctg gacagctggc gcaaagagtt tattaaaagc tttgccgggc atgagattgc
     7081 tgatgtgcag cgtatcagcg aaaaggtgga agctctgtgg aacacctatc gccagcaact
     7141 taaagcagaa cgtctgaaaa ccgccgacag ctacccggtg tggccggcag aaaacagcga
     7201 gcagacgcgt tcttcgctga gcagtaaaga tgaaaccttc agtggtcgtc ttgaagataa
     7261 cagtgcctac cagaagctgc gttgggtgat ggactactgg tgcgcgctgt ggttctggcc
     7321 gatcgacaaa gccgatgagc taccggatcg cggcacctgg ttgtttgaga ttgaaaccct
     7381 gctcgacggg attgtaatca cggaaaaagt cactgaagtt gcggagcaca ccaccggcga
     7441 tctgtttgcc gaagaaggtc tgctgcgaga agagtcttca ctgttttccg gcgcaggtcg
     7501 tctgaaaacc gaggtgttgt tccgtcattt gccgcgtctg gcgattgtcg atgccctgag
     7561 aaagcagtac cgtttcttcc actgggatct ggagttctgc gacctgtttg ccgagcgcgg
     7621 cggttttgac ctgatgctcg gaaacccgcc gtggctaaaa gttgtatggg aagaacgcgg
     7681 aatattggga gattataaac ctgagcttgt actgcacaat cttagcgctg ccaatatatc
     7741 ttctctccga gaagaatctt ttgcacgagt ctatggatta gaagatgcat ggcgaagtga
     7801 gtttgaatct gccgttggat tacagaacta tctaaatagt cagcaaaatt tttcatgctt
     7861 gttagggcaa aaaacaaatc tatataaatg ttttttacca gtctcatggc gacttatttc
     7921 tgaacatggt gtttcagggt tattgcaccc tgatggagtt tatgatgata gcaaggcggg
     7981 ggaacttcgt gccaaaattt actgccgttt gcgtgaccat tatcaatttc aaaatgaatt
     8041 agggcttttt cccgatgttc atcatgcaac aacatttagt attaatattt atggtccatt
     8101 gcaagaatcg ccttcattta cacacatgtc aaatttgttc tctgtgtata caatagattc
     8161 aagtattttg catgataatc aaggggaaat tcctggtatc aaggaagaat cttacaatgg
     8221 ggataaactg aaaataaatt ggaatatatc accccataaa tcaagagtat taacaattac
     8281 tattgatgag ttacgtttgt ttgcggggat atttgatgat aattcaacac ctgcactgca
     8341 cgcaaggctt ccagctttac atacagtaca attattggaa gttttacgta aatttgatgt
     8401 taaatcaaaa aaactatctt caataaaaaa taattattat tctacagtga tgtttgatga
     8461 aacatttgct caaagagatg gtacaactaa aaggaaaacg gaattcccct ctggaatttc
     8521 acaatggata ttatctggac cacatttctt tgtggggaat gcttattaca aaacgccacg
     8581 gactttatgt aaattaaata gtgactatga ctccattgat ttgatgacta ttcctgataa
     8641 ttatatgcct agggttaatt atattcctaa ttgcgagtat tccgattttc taaatcgaat
     8701 tccgacagtt tcttgggatg gtaatgtctt tcctgtgact aatggctata gatttgttaa
     8761 tcgggaaatg ataggttcaa cttctgaacg aactttcatc gcaactataa tacctcccgg
     8821 tatttctcat attaatacgt gtctaagcac tatttttaaa catgactatg atttacttga
     8881 tttcttttcg atgtctttgt ccattgtcgt agattataga gtcaagtgta ctggcatggg
     8941 gcatgcgaat cagtcattag ttaatcaact tccattatta agtaatgaga agtttagagc
     9001 ctctttacat gcaaggtcga tggctttggt tagtataaca gagcattata aaaaattgtg
     9061 gtgttccatt tattcctcag aatttaaaat acaatattgg agccgcgatc tcccacagct
     9121 cccccaggat ttcttcacca atctgacccc agaatggcag cgtaactgcg ccttacgctc
//
LOCUS       DAMZFT010000185         9129 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.185, whole genome shotgun sequence.
ACCESSION   DAMZFT010000185 DAMZFT010000000
VERSION     DAMZFT010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9129)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9129)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9129
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.185"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(<1..495)
                     /locus_tag="PN977_004597"
     CDS             complement(<1..495)
                     /locus_tag="PN977_004597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HDI6044539.1"
                     /translation="MDQKLLTDFRSELLDSRFGAKAISTIAESKRFPLHEMRDDVAFQ
                     IINDELYLDGNARQNLATFCQTWDDENVHKLMDLSINKNWIDKEEYPQSAAIGLRCVN
                     MVADLWHAPAPKNGQAVGTNTIGSSEACMLGGMAMKWRWRKRMEAAGKPTNKPNLVCG
                     PVQIC"
     gene            complement(706..2103)
                     /gene="ccp"
                     /locus_tag="PN977_004598"
     CDS             complement(706..2103)
                     /gene="ccp"
                     /locus_tag="PN977_004598"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HDI6044540.1"
                     /translation="MKMVSRITAIGLAGVAICYLGLSGYVWYHDNKRSKQADVQASAV
                     SENNQVLGFLREKGCDYCHTPSAELPAYYYIPGAKQLMDYDIKLGYKSFNLEAVRAAL
                     LADKPVSQSDLNKIEWVMQYETMPPTRYTALHWAGKVSDEERAEILAWIAKQRAEYYA
                     SNDTAPEHRNEPVQPIPQKLPTDAQKVALGFALYHDPRLSADSTISCAHCHALNAGGV
                     DGRKTSIGVGGAVGPINAPTVFNSVFNVEQFWDGRAATLQDQAGGPPLNPIEMASKSW
                     DEIIAKLEKDPQLKAQFLEVYPQGFSGENITDAIAEFEKTLITPDSPFDKWLRGDENA
                     LTAQQKKGYQLFKDNKCATCHGGIILGGRSFEPLGLKKDFNFGEITAADIGRMNVTKE
                     ERDKLRQKVPGLRNVALTAPYFHRGDVPTLDGAVKLMLRYQVGKELPQEDVDDIVAFL
                     HSLNGVYTPYMQDKQ"
     gene            2508..4157
                     /locus_tag="PN977_004599"
     CDS             2508..4157
                     /locus_tag="PN977_004599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase"
                     /protein_id="HDI6044541.1"
                     /translation="MLNQKIQNPNPDELMIEVDLCYELDPYELKLDEMIEAEPEPEMI
                     EGLPASDALTPADRYLELFEHVQSAKIFPDSKTFPDCAPKMDPLDILIRYRKVRRHRD
                     FDLRKFVENHFWLPEVYSSEYVSDPQNSLKEHIDQLWPVLTREPQDHIPWSSLLALPQ
                     SYIVPGGRFSETYYWDSYFTMLGLAESGREDLLKCMADNFAWMIENYGHIPNGNRTYY
                     LSRSQPPVFALMVELFEEDGVRGARRYLDHLKMEYAFWMDGAESLIPNQAYRHVVRMP
                     DGSLLNRYWDDRDTPRDESWLEDVETAKHSGRPPNEVYRDLRAGAASGWDYSSRWLRD
                     TGRLASIRTTQFIPIDLNAFLFKLESAIANISALKGEKETEALFRQKASARRDAVNRY
                     LWDDENGIYRDYDWRREQLALFSAAAIVPLYVGMANHEQADRLANAVRSRLLTPGGIL
                     ASEYETGEQWDKPNGWAPLQWMAIQGFKMYGDDLLGDEIARSWLKTVNQFYLEQHKLI
                     EKYHIADGVPREGGGGEYPLQDGFGWTNGVVRRLIGLYGEP"
     gene            complement(4208..4810)
                     /locus_tag="PN977_004600"
     CDS             complement(4208..4810)
                     /locus_tag="PN977_004600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDI6044542.1"
                     /translation="MQIVMFDRQSIFIHGMKISLQQRIPGVSIQGAGQADELWQKLES
                     YPEALVMLDGDQDGEFCYWLLQKTVVQFPEVKVLITATDCNKRWLQEVIHFNVLAIVP
                     RDSTVETFALAVNSAAMGMMFLPGDWRTTPEKDIKDLKSLSARQREILTMLAAGESNK
                     EIGRALNISTGTVKAHLESLYRRLEVKNRTQAAMMLNISS"
     gene            5330..6229
                     /locus_tag="PN977_004601"
     CDS             5330..6229
                     /locus_tag="PN977_004601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417978.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDI6044543.1"
                     /translation="MDKIHAMQLFIKVAELESFSRAADFFALPKGSVSRQIQALEHQL
                     GTQLLQRTTRRVKLTPEGMTYYQRAKDVLSNLNELDGLFQQDATSISGKLRIDIPPGI
                     AKSLLLPRLSEFLYLHPGIELELSSHDRPVDILHDGFDCVIRTGALPEDGVIARPLGK
                     LTVVNCASPHYLTRFGYPQSPDDLTSHAIVRYTPHLGVHPLGFEVASVNGVQWFKSGG
                     MLTVNSRENYLAAGLAGLGIIQIPRIAVREALRAGRLIEVLPGYRAEPLSLSLVYPQR
                     RELSRRVNLFMQWLAGVMKEYLD"
     gene            6278..7291
                     /gene="yhjD"
                     /locus_tag="PN977_004602"
     CDS             6278..7291
                     /gene="yhjD"
                     /locus_tag="PN977_004602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000191259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YhjD"
                     /protein_id="HDI6044544.1"
                     /translation="MTQENEIKRPTQDLEHEPIKQLDNSEKGGKVSQALETVTTTAEK
                     VQRQPVIAHLIRATERFNDRLGNQFGAAITYFSFLSMIPILMVSFAAGGFVLASHPML
                     LQDIFDKILQNISDPTLAATLKNTINTAVQQRTTVGLVGLAVALYSGINWMGNLREAI
                     RAQSRDVWERSPQDQEKFWVKYLRDFISLIGLLIALIVTLSITSVAGSAQQMIISALH
                     LNSIEWLKPTWRLIGLAISIFANYLLFFWIFWRLPRHRPRKKALIRGTFLAAIGFEVI
                     KIVMTYTLPSLMKSPSGAAFGSVLGLMAFFYFFARLTLFCAAWIATAEYKDDPRMPGK
                     TQR"
     gene            7683..9005
                     /locus_tag="PN977_004603"
     CDS             7683..9005
                     /locus_tag="PN977_004603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MHS family MFS transporter"
                     /protein_id="HDI6044545.1"
                     /translation="MQATATTLDHEQEYTPINSRNKVLVASLIGTAIEFFDFYIYATA
                     AVIVFPHIFFPQGDPTAATLQSLATFAIAFVARPIGSAVFGHFGDRVGRKATLVASLL
                     TMGISTVVIGLLPGYATIGIFAPLLLALARFGQGLGLGGEWGGAALLATENAPPRKRA
                     LYGSFPQLGAPIGFFFANGTFLLLSWLLTDEQFMSWGWRVPFIFSAVLVIIGLYVRVS
                     LHESPVFEKVAKAKKQVKIPLGTLLTKHVRVTVLGTFIMLATYTLFYIMTVYSMTFST
                     AAAPVGLGLPRNEVLWMLMMAVIGFGVMVPVAGLLADAFGRRKSMVIITTLIILFALF
                     AFNPLLGSGNPILVFAFLLLGLSLMGLTFGPMGALLPELFPTEVRYTGASFSYNVSSI
                     LGASVAPYIAAWLQTNYGLGAVGLYLAAMAGLTLIALLLTHETRHQSL"
BASE COUNT         2225 a         2341 c         2206 g         2357 t
ORIGIN      
        1 gcagatttgt accggaccgc acaccaggtt tggtttgttg gtcggcttac ctgcggcttc
       61 catacgcttg cgccaacgcc atttcatcgc catcccgccg agcatacagg cctcggaaga
      121 accaatggtg ttggtgccaa cggcctgacc atttttcggt gcaggcgcat gccacagatc
      181 ggcaaccatg tttacgcaac gcaggccgat ggctgcagat tgcggatatt cttctttgtc
      241 gatccagttt ttattgatcg acaaatccat caatttatgg acgttttcgt cgtcccaggt
      301 ctggcagaaa gtggccaggt tctgacgagc gttgccatca agatataatt catcattgat
      361 aatctgaaat gcaacatcat ctcgcatttc gtgcagcgga aatcgttttg actccgcgat
      421 agtagaaatg gcctttgcgc caaaacgtga atcgagtagt tctgagcgga aatccgttaa
      481 cagcttctgg tccatttcga actccttaaa tttatttgaa ggcaataaaa aagtaggatt
      541 tatccgcaat ggaagcaagg tattacaggc ttaatttaaa taacaaaatc ctaagcagaa
      601 aaacgacttt ttataatata aacatttaac atgataatat taaaaacaga ctttattatc
      661 gtaataaaaa tcgcccaaac attcctgttc gggcgattaa attaattatt gtttatcctg
      721 catatacggc gtgtaaaccc cgttcagact gtgcaggaaa gctacgatat catccacatc
      781 ctcctgcggc agctctttgc ctacctgata gcgcaacatc agtttcaccg ccccgtccag
      841 cgtcggcaca tcaccgcgat ggaagtacgg tgccgttagt gcaacgttac gtaaaccggg
      901 tactttctga cgcaatttat cgcgctcttc tttagtcaca ttcatacgac caatatccgc
      961 cgccgtaatt tcaccaaagt taaagtcttt tttcagcccc aacggttcga aggaacgtcc
     1021 gccgagaata ataccaccat gacaagttgc acatttatta tctttaaata attgatagcc
     1081 ttttttctgt tgtgccgtca gcgcattctc atctccacgc aaccatttat caaatgggga
     1141 atccggcgta attaatgttt tttcaaattc agcaatggca tcagtaatat tttcaccgct
     1201 gaaaccttgc ggatagactt cgaggaactg cgctttaagc tgcggatctt tttccagctt
     1261 agcaataatt tcgtcccagg atttcgacgc catttcaatc gggttcaacg gcggtccacc
     1321 cgcctgatcc tgcaatgttg ccgcacgacc atcccagaac tgctcaacgt taaatactga
     1381 gttaaatacc gtcggcgcgt taatcggccc aactgcgcca ccaacaccaa tcgatgtttt
     1441 tctgccatcg acgccccccg cattcaacgc atggcaatga gcgcatgaaa tggtgctatc
     1501 agccgataaa cggggatcgt gatacagcgc aaagcccaac gccacttttt gcgcatcggt
     1561 aggcagtttt tgcgggatgg gctgcaccgg ttcattgcga tgctccggag cagtatcatt
     1621 actggcgtaa tattctgcgc gttgttttgc aatccaggcg agtatttccg cccgctcttc
     1681 atcactcacc ttacccgccc agtgtagcgc ggtataacgc gttggtggca tggtttcata
     1741 ctgcattacc cattcaatct tattcagatc gctttgcgaa acgggtttat cagccagcag
     1801 agccgcacgc acggcctcga ggttaaaaga tttatatcca agcttaatgt cgtaatccat
     1861 caactgtttc gcgccaggaa tataataata ggcgggtaat tctgccgaag gcgtatggca
     1921 atagtcgcat cctttttcgc gaagaaaacc taaaacctga ttattttcac tgacagcaga
     1981 tgcctgaaca tcggcctgct tactgcgttt attatcgtgg taccacacat aaccagataa
     2041 ccctaaatag caaatcgcga cgccagccag gccgatcgcg gtaatacgtg agaccatttt
     2101 cattattctt ccctcacggt ggtgagttat tgtgacccag aaaattttcc agaatgctgc
     2161 aaacgtctct gcgagacaaa gccatcataa aaaacaggga aatacgcttt ttgataggaa
     2221 tcaatcaatt acatgaatat aaactatcaa taagatagcc tgtgcctatt acccagatca
     2281 ggaatggcat atcatgattg cggcgagagc aggattgaat gttctgaaaa tgaaaaacaa
     2341 cacgaaatcc gtatttttat aaattccatt gatattagtg cgttatattt tttggcaggt
     2401 taataaacgt gatccaccgc acgctttgtc gcccaccagg cggagcgaat gactaccctt
     2461 aaagaaaagc ccgataatta gcgacgaatt tcggaggtcg gatccttatg ctcaatcaga
     2521 aaattcaaaa ccctaatcca gacgaactga tgatcgaggt cgatctctgc tatgagctgg
     2581 acccgtatga attaaaactg gatgagatga tcgaggcaga accggaaccc gagatgattg
     2641 aagggctgcc cgcctctgat gcgctgacgc ctgccgatcg ctatctcgaa ctgttcgagc
     2701 atgttcagtc ggcgaaaatt ttccccgaca gtaaaacctt tcccgactgc gcacctaaaa
     2761 tggacccgct ggatatttta atccgctacc gtaaagtgcg ccgtcatcgt gattttgact
     2821 tgcgcaagtt tgttgaaaac cacttctggc tgccggaggt ctactccagc gagtatgtat
     2881 cggacccgca aaattccctg aaagagcata tcgaccagct gtggccggtg ctaacccgcg
     2941 aaccgcagga tcacattccg tggtcttctc tgctggcgct gccgcagtca tatattgtcc
     3001 cgggcggccg ttttagcgaa acctactatt gggattccta tttcaccatg ctggggctgg
     3061 cggaaagtgg tcgggaagat ttgctgaaat gcatggccga taacttcgcc tggatgatcg
     3121 aaaactacgg tcacatcccc aacggcaacc gcacctatta tttgagccgc tcgcaaccac
     3181 cggtttttgc gctgatggtg gagttgtttg aagaagatgg tgtacgcggc gcgcgccgct
     3241 atctcgacca cctgaaaatg gaatatgcct tctggatgga cggtgcagaa tcgttgatcc
     3301 ctaatcaggc ctatcgccat gttgtgcgga tgccggacgg atcgctgctc aaccgttact
     3361 gggacgatcg cgacacgccg cgtgacgaat cctggcttga agacgttgaa accgcgaaac
     3421 attctggtcg cccgcctaac gaggtgtacc gcgatttacg tgcgggggcg gcctccggtt
     3481 gggattactc ttcccgttgg ctgcgtgata ctggtcgtct ggcgagcatt cgtaccaccc
     3541 agttcatccc catcgatctg aatgccttcc tgtttaaact ggagagcgcc atcgccaaca
     3601 tctcggcgct gaaaggcgag aaagagacag aagcactgtt ccgccagaag gccagtgccc
     3661 gtcgcgatgc ggtaaaccgt tacctctggg atgatgaaaa cggtatctac cgcgattacg
     3721 actggcgacg cgagcaactg gcgctgtttt ccgctgccgc cattgtgccg ctctatgtcg
     3781 gtatggcgaa ccatgaacag gccgatcgtc tggcaaacgc cgtgcgcagc cgattactga
     3841 cacctggcgg gattctggca agcgagtacg aaaccggtga acagtgggat aaacccaacg
     3901 gctgggcacc gttacaatgg atggcgattc agggatttaa aatgtatggc gatgaccttc
     3961 tgggtgatga aatcgcgcgc agctggctga agacggtgaa tcagttctat ctggaacagc
     4021 acaaactgat cgagaaatac catattgccg atggtgtccc tcgcgaaggc ggcggtggcg
     4081 agtatccgtt gcaggatggg tttggctgga ctaacggtgt ggtgcgccgt ttaattggtt
     4141 tgtacggtga accataatat ttttacagcc agccgctaac ttcctgctgg ctgtaaaatt
     4201 atcctcttca ggaggagata tttaacatca ttgccgcctg ggtgcgattt ttcacttcca
     4261 gacggcgata cagggattcc agatgcgctt ttaccgttcc ggtactgata ttcagcgccc
     4321 tgccgatctc cttatttgat tcgcccgccg ctaacatggt taaaatctcc cgctggcggg
     4381 cgcttaacga tttgagatct ttaatgtcct tttccggcgt cgttcgccag tctcccggca
     4441 gaaacatcat ccccatcgcc gcactattta ccgccaacgc aaatgtctca acggttgaat
     4501 cacgaggcac aatggccagc acattaaaat ggataacttc ctgtaaccac cgtttattgc
     4561 aatccgtcgc cgtaattaac accttaacct caggaaactg caccacggtt ttttgcagca
     4621 accagtagca aaactcacca tcctgatcgc catcgagcat aactaaggct tcagggtaac
     4681 tttccagctt ttgccataac tcgtctgcct gaccggcccc ctgaatactc actcctggaa
     4741 tacgctgctg taaactgatt ttcattccat gaataaatat tgactgcctg tcaaacatga
     4801 ctatttgcat aactgaatct ccacctgaat acgttaaaaa gacttaagta gtggaagggt
     4861 attacccgcg agaaaaaata agaattcgcc atttggcggt ggccattcta cagagatgac
     4921 gcgtagaaaa tagttaccga tataaatagt tacagctaaa cgcctgaaat tacatgtcga
     4981 gggcactatt taaaacaatt ttgaggattt ccttatattg atggttagta cgcatgcaat
     5041 taaaaatgaa actccgcgac cacaagcaaa aataacaaat ggcaatgaga caaaaataag
     5101 cacaaatagc caacacgtcc tctgttcact ttaaagggaa tcgctgaaaa aaacgttctg
     5161 tttagggaaa ttcacctttc tcataaagct attccgccct tttcctgctg agaaatcgcc
     5221 acattcgtca tgacaacatt gtgaaacccg gcattagatg ttagaaaaaa caataaacaa
     5281 tgcgatatgc gcgctactgt ttcgctgtga acaacaatcg gtcaaagaaa tggataaaat
     5341 tcacgcaatg cagttgttca tcaaagtcgc tgagctggaa agtttttccc gcgcagcgga
     5401 tttctttgct ttgccaaagg gaagtgtttc gcgccagata caggcactgg aacatcaact
     5461 tggcacccag cttctccagc gcaccacgcg gcgggtcaaa ctcacgccag aaggcatgac
     5521 ctattatcaa cgggcaaaag atgtgttgag taatctcaac gaactggacg gtctgtttca
     5581 acaggatgcc accagtatca gcggtaaatt acgcatcgac atcccgccag gaatcgcgaa
     5641 aagcctgtta ctgccgcgcc tgtcggaatt tctctatctg catccgggaa ttgagctgga
     5701 gctgagtagc catgaccgtc cggtagatat tcttcacgat ggttttgatt gcgtgatacg
     5761 cactggcgca ttaccggaag atggcgttat cgcccgtccc ctcggcaaac tgaccgtggt
     5821 caattgtgcc agtccacact atctgacgcg ctttggttat cctcaaagcc ccgacgatct
     5881 gacttcgcac gcaatagtgc gttacacacc gcacctgggt gtacatccgt taggttttga
     5941 ggttgccagc gttaatggcg tccagtggtt taagtctggc ggcatgttga cggtaaacag
     6001 tcgcgaaaac tatctcgccg ccggtcttgc cggtctgggg attattcaga tcccgcgcat
     6061 tgccgtgcgc gaagccctgc gtgccgggcg gcttattgaa gtgttgcctg gctaccgtgc
     6121 cgagccgctg tccctttcac tggtttatcc gcagcgtcgg gagctttccc ggcgtgtaaa
     6181 cctgtttatg cagtggctgg ctggcgtaat gaaagagtac ctggactgac cgactatact
     6241 ttttaagaat gaccacgaca gaaggacaaa agagcggatg acgcaggaaa acgagatcaa
     6301 acgtcccacc caggatctgg agcacgagcc gattaagcag ttggataata gcgagaaagg
     6361 cggcaaagtt agccaggcgc tggaaaccgt caccactact gccgagaaag tccaacgcca
     6421 accggttatt gcgcacctga ttcgcgcgac agaacgcttt aacgatcggc tgggtaacca
     6481 gtttggtgcg gctatcacct atttctcatt tttgtcgatg atcccgattt tgatggtgtc
     6541 gtttgccgca gggggctttg tgctggcctc ccatccgatg ttgctacagg atatcttcga
     6601 caaaattctg caaaacatca gcgatccgac gctagccgcc acgttgaaaa acaccatcaa
     6661 caccgccgtt cagcagcgta cgactgtagg gcttgtcggc ctggcggtgg cactttattc
     6721 cggcatcaac tggatgggta acctgcgtga agcgattcgt gcccagtcgc gcgatgtctg
     6781 ggaacgctcg ccgcaagatc aggagaagtt ctgggtaaaa tatctgcgtg attttatttc
     6841 gctgattggt ttgttgattg cgctgattgt gacgctttct atcacctcgg ttgccggttc
     6901 tgcgcagcaa atgattatta gcgccctgca cctgaacagc attgagtggc tgaaaccgac
     6961 gtggcggttg attggcctgg cgatttccat cttcgccaac tatctgcttt tcttctggat
     7021 cttctggcgg ttaccgcgcc accgtccgcg caaaaaagcc ctgattcgcg gaacatttct
     7081 ggctgctatt ggttttgaag tgattaaaat tgtgatgact tacaccctgc catcgctgat
     7141 gaaatccccc tctggcgcag catttggttc cgtgctgggg ctgatggcat ttttctactt
     7201 cttcgcccgt ttgacgctgt tttgcgcggc gtggattgcc accgccgaat ataaagacga
     7261 cccgcgaatg ccggggaaaa cgcagcggta attcattggt ttataatgcc tgataaacgc
     7321 acggtcgatc ccctcgcccc ttcggggaga ggattagggt gagggggtac aagccagcca
     7381 gagaccaggc aatgacatgc acataaccac ctgaaactac atctcttcag gagcaaacaa
     7441 tttcattcca actcataacc ccagcatata aatccagttg gtaactttta tttaacctga
     7501 aaccagtttt atccactatt tataaaatta tgtgaagcat ttcatagaag aaaaatcact
     7561 ggcctaaaca ttatcccctt tttgcctgat ttttgaccat ttccgcgatt tgttacacat
     7621 tgaaatatca cttttgctgt gcgtaatatg gctattcgtt agccaaaaaa taagaaaaga
     7681 ttatgcaagc aacagccaca acactcgacc acgagcaaga atacacgccg atcaactcgc
     7741 gtaataaagt ccttgtcgcc tctctcattg gcacggccat tgagttcttc gacttttaca
     7801 tttacgccac tgcggccgtt attgtgtttc cgcatatctt cttcccgcag ggtgatccta
     7861 cggcagcaac gctacagtcg cttgccacct tcgccatcgc cttcgtcgcg cgccccattg
     7921 gctctgccgt ttttggtcat tttggcgatc gcgttgggcg taaagcgacg ctggtcgcct
     7981 cgttgctaac gatggggatt tcgaccgtgg tgattggtct gctgccgggc tatgccacga
     8041 ttggtatttt cgccccgctg ctgctggcgc tggctcgatt tggtcagggt ctgggtttag
     8101 gcggcgaatg gggcggcgcg gcgctgctgg cgactgaaaa cgccccaccg cgcaaacgtg
     8161 cactgtatgg ctccttcccg cagctgggcg caccgattgg cttcttcttt gccaacggca
     8221 ctttcttgct gctttcctgg ctactgaccg acgagcagtt tatgagctgg ggctggcgcg
     8281 tgccgtttat cttctcggcg gtgctggtca ttatcggcct gtatgttcgc gtgtcgctgc
     8341 atgagtcgcc ggtgtttgag aaagtcgcta aagcgaaaaa acaggtgaag atcccgctgg
     8401 gtacgctgct gaccaaacat gttcgcgtaa ccgtactggg tacgttcatt atgctggcaa
     8461 cctatacgct gttttacatc atgacggtct actctatgac ctttagtacc gccgccgcgc
     8521 cagttgggct tggcctgccg cgtaacgaag tgttgtggat gctgatgatg gcagttattg
     8581 gttttggcgt gatggtgcca gtcgccggat tactggctga tgcctttggt cgccgtaaaa
     8641 gcatggtaat catcaccacg ctgatcatcc tgttcgcgct gttcgccttt aacccgttgc
     8701 tcggttctgg caacccgatt ctggtttttg ccttcctgct gctggggtta agtctgatgg
     8761 ggctgacctt cggtccgatg ggcgcgctgt taccagagct gttcccgaca gaagtgcgtt
     8821 acaccggagc atcgttctct tacaacgtat cgtcgattct cggggcttcc gttgcgccgt
     8881 atatcgcagc ctggttgcag actaactacg ggttaggtgc ggtggggcta tatctggcgg
     8941 cgatggctgg cttgacgtta atcgccctgc tgctgaccca tgagacgcga catcagtcgt
     9001 tgtaataaga gtgaaaagcc tgatgcgcta cgcttatcag gccagaattt ccatacaata
     9061 tattgaatta gcaaggattt gtaagccgga ggcgttcacg ccgcatccgg catgaacaaa
     9121 gcacacttt
//
LOCUS       DAMZFT010000186         9081 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.186, whole genome shotgun sequence.
ACCESSION   DAMZFT010000186 DAMZFT010000000
VERSION     DAMZFT010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9081)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9081)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9081
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.186"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(526..737)
                     /locus_tag="PN977_004604"
                     /pseudo
     CDS             complement(526..737)
                     /locus_tag="PN977_004604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310697.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase family
                     protein"
     gene            complement(891..1187)
                     /locus_tag="PN977_004605"
     CDS             complement(891..1187)
                     /locus_tag="PN977_004605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HDI6044546.1"
                     /translation="MLYVIYAQDKADSLEKRLSVRPAHLARLQLLHDEGRLLTAGPMP
                     AVDSNDPGAAGFTGSTVIAEFESLEAAQAWADADPYVAAGVYEHVSVKPFKKVF"
     gene            1411..2115
                     /gene="tonB"
                     /locus_tag="PN977_004606"
     CDS             1411..2115
                     /gene="tonB"
                     /locus_tag="PN977_004606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein TonB"
                     /protein_id="HDI6044547.1"
                     /translation="MTLDLPRRFPWPTLLSVCIHGAVVAGLIYTSVHQVIELPAPAQP
                     ISVTMVAPADLEPPQAVQPPPEPVVEPEPEPEPIPEPPKEAPVVIEKPKPKPKPVKKV
                     EQPKRDVKPVESRPASPFENTAPARPTSSTATAATSKPVTSVASGPRALSRNQPQYPA
                     RAQALRIEGQVKVKFDVTPDGRVDNVQILSAKPANMFEREVKNAMRRWRYEPGKPGSG
                     IVVNILFKINGTTEIQ"
     gene            complement(2155..2553)
                     /gene="yciA"
                     /locus_tag="PN977_004607"
     CDS             complement(2155..2553)
                     /gene="yciA"
                     /locus_tag="PN977_004607"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioester hydrolase YciA"
                     /protein_id="HDI6044548.1"
                     /translation="MSTTHNVPQGDLVLRTLAMPADTNANGDIFGGWLMSQMDIGGAI
                     LAKEIAHGRVVTVRVEGMTFLRPVAVGDVVCCYARCVQKGTTSVSINIEVWVKKVASE
                     PIGQRYKATEALFKYVAVDPEGKPRALPVE"
     gene            complement(2658..3197)
                     /locus_tag="PN977_004608"
     CDS             complement(2658..3197)
                     /locus_tag="PN977_004608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein A"
                     /protein_id="HDI6044549.1"
                     /translation="MKQFLDFLPLVVFFAFYKIYDIYAATAALIVATAIVLIYSWVRF
                     RKVEKMALITFVLVVVFGGLTLFFHNDEFIKWKVTVIYALFAGALLVSQWVMKKPLIQ
                     RMLGKELTLPQSVWSKLNLAWAVFFILCGLANIYIAFWLPQNIWVNFKVFGLTALTLI
                     FTLLSGIYIYRHMPQEDKS"
     gene            complement(3227..3970)
                     /locus_tag="PN977_004609"
     CDS             complement(3227..3970)
                     /locus_tag="PN977_004609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0259 family protein"
                     /protein_id="HDI6044550.1"
                     /translation="MSITAQSVYRDTGNFFRNQFMTILLVSLLCAFITVVLGHVFSPS
                     DAQLAQLNDGVPVSGSSGLFDLVQNMSPEQQQILLQASAASTFSGLIGNAILAGGVIL
                     IIQLVSAGQRVSALRAIGASAPILPKLFILIFLTTLLVQIGIMLVVVPGIIMAILLAL
                     APVMLVQDKMGIFASMRSSMRLTWANMRLVAPAVLSWLLAKTLLLLFASSFAALTPEI
                     GAVLANTLSNLISAVLLIYLFRLYMLIRQ"
     gene            complement(3998..4087)
                     /locus_tag="PN977_004610"
                     /pseudo
     CDS             complement(3998..4087)
                     /locus_tag="PN977_004610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460692.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
     gene            4325..4963
                     /gene="ompW"
                     /locus_tag="PN977_004611"
     CDS             4325..4963
                     /gene="ompW"
                     /locus_tag="PN977_004611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpW"
                     /protein_id="HDI6044551.1"
                     /translation="MKKLTVAALAVTTLLSGSAYAHEAGEFFMRAGSATVRPTEGAGG
                     TLGSLGGFSVTNNTQLGLTFTYMATDNIGVELLAATPFRHKIGTRATGDIATVHHLPP
                     TLMAQWYFGDASSKFRPYVGAGINYTTFFDNGFNDHGKEAGLSDLSLKDSWGAAGQVG
                     VDYLINRDWLVNMSVWYMDIDTTANYKLGGAQQHDSVRLDPWVFMFSAGYRF"
     gene            complement(5023..5528)
                     /locus_tag="PN977_004612"
                     /pseudo
     CDS             complement(5023..5528)
                     /locus_tag="PN977_004612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309856.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
     gene            complement(5574..6074)
                     /locus_tag="PN977_004613"
     CDS             complement(5574..6074)
                     /locus_tag="PN977_004613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HDI6044552.1"
                     /translation="MNMKTIEDVFIHLLSDTYSAEKQLTRALAKLARATSNEKLSQAF
                     HAHLEETHGQIERIDQVVESESNLKIKRMKCVAMEGLIEEANEVIESTEKNEVRDAAL
                     IAAAQKVEHYEIASYGTLATLAEQLGYRKAAKLLKETLEEEKATDIKLTDLALNNVNK
                     KAENKA"
     gene            complement(6160..6339)
                     /locus_tag="PN977_004614"
     CDS             complement(6160..6339)
                     /locus_tag="PN977_004614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309858.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="HDI6044553.1"
                     /translation="MAEHRGGSGNFAEDREKASDAGRKGGQHSGGNFKNDPQRASEAG
                     KKGGQQSGGNKSGKS"
     gene            complement(6720..7526)
                     /gene="trpA"
                     /locus_tag="PN977_004615"
     CDS             complement(6720..7526)
                     /gene="trpA"
                     /locus_tag="PN977_004615"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HDI6044554.1"
                     /translation="MERYESLFARLKERKEGAFVPFVTLGDPGIEQSLKIIDALIEAG
                     ADALELGIPFSDPLADGPTIQNATLRAFAAGVTPAQCFEMLALIRQKHPTIPIGLLMY
                     ANLVFNKGIDEFYAQCEKAGVDSVLVADVPVEESAPFRQAALRHNVAPIFICPPNADD
                     DLLRQIASYGRGYTYLLSRAGVTGAENRAALPLNHLVTKLKEYNAAPPLQGFGISAPD
                     QVKAAIDAGAAGAISGSAIVKIIEQHINEPEKMLAALKAFVQPMKAATRS"
     gene            complement(7526..8719)
                     /gene="trpB"
                     /locus_tag="PN977_004616"
     CDS             complement(7526..8719)
                     /gene="trpB"
                     /locus_tag="PN977_004616"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HDI6044555.1"
                     /translation="MTTLLNPYFGEFGGMYVPQILMPALRQLEEAFVSAQKDPEFQAQ
                     FNDLLKNYAGRPTALTKCQNITAGTNTTLYLKREDLLHGGAHKTNQVLGQALLAKRMG
                     KTEIIAETGAGQHGVASALASALLGLKCRIYMGAKDVERQSPNVFRMRLMGAEVIPVH
                     SGSATLKDACNEALRDWSGSYETAHYMLGTAAGPHPYPTIVREFQRMIGEETKAQILE
                     REGRLPDAVIACVGGGSNAIGMFADFINETDVGLIGVEPGGHGIETGEHGAPLKHGRV
                     GIYFGMKAPMMQTEDGQIEESYSISAGLDFPSVGPQHAYLNSTGRADYVSITDDEALE
                     AFKTLCLHEGIIPALESSHALAHALKMMRENPEKEQLLVVNLSGRGDKDIFTVHDILK
                     ARGEI"
     gene            complement(8731..>9081)
                     /locus_tag="PN977_004617"
     CDS             complement(8731..>9081)
                     /locus_tag="PN977_004617"
                     /EC_number="4.1.1.48"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707171.1"
                     /note="monomeric bifunctional protein; functions in
                     tryptophan biosynthesis pathway;
                     phosphoribosylanthranilate is rearranged to
                     carboxyphenylaminodeoxyribulosephosphate which is then
                     closed to form indole-3-glycerol phosphate; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional indole-3-glycerol phosphate
                     synthase/phosphoribosylanthranilate isomerase"
                     /protein_id="HDI6044556.1"
                     /translation="EDQLYIDTLREALPAHVAIWKALSVGETLPARDFQHIDKYVFDN
                     GQGGSGQRFDWSLLNGQSLGNVLLAGGLGADNCVEAAQTGCAGLDFNSAVESQPGIKD
                     ARLLASVFQTLRAY"
BASE COUNT         2439 a         2136 c         2150 g         2356 t
ORIGIN      
        1 gcgacactca aattgaatcc agaaacaatg tcattaaatg ataaaggctc accggttaac
       61 gccattttag tactgttcac aaacatcaat gttataattg cgatgcctga taaataagaa
      121 catagaaaaa ttggaatgtt tattattcta aataacaaat aaataaacaa aatgggggat
      181 gaacgccaga tgaaattcaa tattgcacca attaactctt taggcggttc aatatttcct
      241 atgataacta ttgactgatt tagaaaacaa aataagtaca ataaaaacat aacgttgaca
      301 atgtcgaaaa catatgattt caactttaag tttttatatc ttgtaacgac tttgctcaaa
      361 ccgatactca tattaatgct ctatctctag aataatgaac ttttatgcag aatatcacaa
      421 aatgacattt tggttaaata aatcatggta gtctctctta ctaatcatga gtttaaaatc
      481 taattagtag agtatctaat ctgtctttgg tatgtgtaaa aatccaggtt tatcccacca
      541 tccggggcgt atccccagaa aactggatca agatggagtt tctcgtcacg ttcgctcaga
      601 agcagaactt tggtgcctgg cgcaacgttg aaacgtccga tcggttcagg gtcatatgga
      661 atgtcttgct cgacttcatc tgcgagaagg gcgagatagt cttcacgcgt cattgactgt
      721 gaaaagcgtc cgcacataga aacctccagc cagatgtcag actgaaagta taagtcgtag
      781 cgaaaaaaaa aggggcgctt agattaatgt gtacttaaat gcgcccttta ttattgatta
      841 tgttgactgt ctttcgttat tgctttgaat ttaaataata tttaatttat tcaaaacact
      901 ttcttaaatg gtttcactga aacgtgttca tagactcctg ccgctacgta cgggtcagca
      961 tcggcccagg cctgagctgc ttccagcgac tcaaattcag caataacggt tgagccagta
     1021 aatcccgcag cccctggatc gttactgtct actgctggca ttggaccggc ggtcaacaaa
     1081 cgaccttcat catgcagtaa ctgtaaacgt gctaaatgtg ccggacgaac ggaaaggcgt
     1141 ttttcgaggc tatcagcttt gtcttgagcg taaataacat acaacacagg cacaactcct
     1201 tgttcgggaa agttgtaaag tacgttattt gaaagggcga agatctgcaa cggaaagatg
     1261 atgtctttgt taagaccatg cataaagtaa ggtcaattac gccaaaaatg acattttcac
     1321 tgatcctgat cgtcttgcct tattgaatat gattgctatt tgcatttaaa atcggggcct
     1381 ggtttttcta ctgaaatgat tatgacttca atgacccttg atttacctcg ccgcttcccc
     1441 tggccgacgt tactttcggt ctgcattcat ggtgctgttg tggcgggttt gatctatacc
     1501 tcggtacatc aggttattga actacctgcg cccgcgcagc cgatttctgt cacgatggtt
     1561 gcgccagcgg atctcgaacc gccacaagcc gttcaaccac caccggagcc ggtggtagag
     1621 ccagaaccgg aacctgagcc gatccctgaa ccgccaaaag aagctccggt ggtcattgaa
     1681 aagccgaagc caaaaccgaa gccggtgaaa aaggtggagc agccaaaacg cgatgtcaaa
     1741 cccgtagagt cgcgtccggc atcaccgttt gaaaatacgg cacccgcgcg cccgacatca
     1801 agtacggcaa cggctgcaac cagcaagccg gttaccagtg tggcttcagg accacgcgca
     1861 ttaagccgta atcagccgca gtatccggca cgagcacagg cattgcgcat tgaagggcag
     1921 gttaaagtta aatttgatgt cacgccagat ggtcgcgtgg ataacgtaca aatcctctca
     1981 gccaagcctg cgaacatgtt tgagcgtgaa gtgaaaaatg cgatgcgcag atggcgttat
     2041 gagccgggta agccaggcag tgggattgtg gtgaatatcc tgtttaaaat taacggcact
     2101 accgaaattc agtaagcata aagtcaaaag cctccgaccg gaggcttttg actattactc
     2161 aacaggtaag gcgcgaggtt ttccttcagg atcaaccgcg acatacttaa ataatgcttc
     2221 tgtcgcttta tagcgttgcc cgatgggttc agacgcgact tttttcaccc acacttcaat
     2281 attaatgctg accgatgtcg tccctttctg gacacagcgt gcatagcagc acaccacatc
     2341 gccgaccgca accggtcgta agaaagtcat tccttcaacc cgcacggtca ctacacgacc
     2401 gtgggcaatt tctttcgcca gaatagcgcc gccaatatcc atttgtgaca ttaaccaacc
     2461 accaaagatg tcaccattgg cattggtatc ggcgggcatg gctaaagtac gtaaaacaag
     2521 atcgccctga gggacgttat gtgttgtaga catggtaaaa ccgacttaaa aggaaaatca
     2581 caggcgcgat gctactatga ttttcctgag gagaacagag gagatacagc gccagccccg
     2641 aaggactggc agtctggtta ggatttatct tcctgcggca tgtggcggta gatatagata
     2701 ccgcttaaca atgtgaagat taaggtaagg gcggtcaggc caaagacttt aaagttgacc
     2761 caaatatttt gcggcagcca gaatgcgatg tagatgtttg ccaggccgca aaggataaag
     2821 aaaacagccc aggccagatt cagcttcgac cataccgatt gcggcagggt gagttcttta
     2881 cccagcatcc gctgaattag cggctttttc atcacccatt gactgactaa cagggcaccc
     2941 gcaaacaggg cataaatgac agtaaccttc catttaataa actcatcatt gtggaagaac
     3001 agcgttaagc caccgaagac gaccaccaga acaaaagtga tcagggccat cttctcaacc
     3061 ttacgaaagc gaacccagct atagataagc acaatcgccg tggcgacgat gagcgccgca
     3121 gtagccgcat agatgtcata aatcttgtag aacgcgaaaa agacaaccag cggtaaaaaa
     3181 tcaagaaact gcttcatttt acgattccgt aatcaaatgc ttaaggttat tggcgaatca
     3241 acatatacag gcggaacaga tagatgagca atacggctga aatcaggttg ctcaaggtgt
     3301 tcgccagtac ggcaccaatt tccggggtta atgcggcaaa agaagaggca aaaagcagca
     3361 acagtgtttt tgccagcaac cagctcagta ctgcgggtgc caccagacgc atattcgccc
     3421 atgtcagccg catactgcta cgcatcgagg caaaaatgcc cattttgtcc tgtaccaaca
     3481 tcaccggtgc cagagccagt aaaatggcca tgataattcc cggaacgacc accagcatga
     3541 tgccaatctg tactaaaagg gtagtcagaa aaatcagaat aaataacttt ggcaatatcg
     3601 gcgcactggc accaatagct cgaagcgcac tgactctctg acccgcagac accagctgga
     3661 taattaatat tacgcctccg gctagaatgg cgttaccgat taatcctgaa aaagtggacg
     3721 ccgctgaagc ctgcagcaaa atttgttgct gttcaggtga catattctga accaggtcga
     3781 acaacccact actgccgcta acgggcacgc cgtcattgag ctgagcaagc tgtgcatcac
     3841 tgggtgagaa aacatgccct aacaccactg tgataaacgc acatagcaac gataccaaca
     3901 gaatggtcat aaattgatta cggaagaaat ttccggtgtc acggtatacg gactgcgccg
     3961 tgatagacat gcactctcct tgagttttat gcaggtgtta attagcgggc aattgtaccc
     4021 tggttaagcc tcgggtggca gcatcaggtc ttgtatggaa aagcatatct ttgtaaagcg
     4081 gaggtaattg cgggtctaag ctatgggcaa accgcagcaa gtggcgagcg agttaatgtt
     4141 tgcgtagtga aatatatagg aattgattta aatcacatta accaggattc acagtgcaac
     4201 ttctaaatta atccagatca ataaagggtg aattatcata tataatgtga tctgtgtagg
     4261 atcatttgtt actccaatgt gggtatattc gtcacgtttt tataaccata acgacggagc
     4321 ggatatgaaa aagttaacag tggcggcttt ggcagtaaca actcttctct ctggcagtgc
     4381 ctatgcgcat gaagcaggcg aattttttat gcgtgcaggg tctgcaaccg tacgtccaac
     4441 agaaggtgct ggtggtacgt taggaagtct gggtggattc agcgtgacca ataacacgca
     4501 actgggcctg acgtttactt atatggcgac cgacaacatt ggtgtggaat tactggctgc
     4561 gacgccgttc cgccataaaa tcggcacccg ggcgaccggc gatattgcaa ccgttcatca
     4621 tctgccgcca acactgatgg cgcagtggta ttttggtgat gccagcagca aattccgtcc
     4681 ttacgttggg gcaggtatta actacaccac cttctttgat aatggattta acgatcatgg
     4741 caaagaggcg gggctttccg atctcagtct gaaagattcc tggggagctg ccgggcaggt
     4801 gggggttgat tatctgatta accgtgactg gttggttaac atgtcagtgt ggtacatgga
     4861 tatcgatacc accgccaatt ataaactggg cggtgcacag caacacgata gcgtacgcct
     4921 cgatccgtgg gtgtttatgt tctcagcagg atatcgtttt taattccgca caaaaacgac
     4981 cccgtaatat acggggtcaa taaggacatg gtataaaggg tattatttct tcgcttctac
     5041 gccatcagtt tcagagcgaa ttaaaaattt ctcagttgtt tgcggaatat gctgaatcag
     5101 ccagtcggcc atttgctttt cctcatttaa aatcgcttcg atggttggaa ttgaagctgt
     5161 atcaccggca ttttttgctg ctgctaagag tgaggtgtaa caggcgattt caaattgctc
     5221 gaagacatat ccgctaatag agcctttgac tatttcatca gaaggaaata taccactgat
     5281 tgactgccca agcgcagcca ttttactcat ggaatcttta atgactgaac gtgaaatgtc
     5341 attacgatca agaatagttt ccagttgaac aatcggtttt tggtttcact aagatgttgt
     5401 tcaatacgag cgcgtagttc aggataatta tctatacggc tggccatgga ttaaagcata
     5461 gattcggctt gcttttccat tgcgtgggcg tcacgtaacc agtcatgata atgttcaata
     5521 cgattcatga aaatgtcctc tttataaaat gactaaaagt taaagttcat atttcaggct
     5581 ttattttcgg ctttcttatt tacgttatta agggccagat cagtcagttt gatgtcggtg
     5641 gccttttctt cttcaagagt ttctttcaga agcttagctg ctttacggta acctaattgt
     5701 tcagccagcg tcgctaatgt cccgtaactg gcaatctcat aatgctcgac tttctgtgct
     5761 gcggcaatca gtgcggcatc acgcacttcg tttttctcgg tactttcgat gacctcatta
     5821 gcttcttcaa taagaccttc cattgccaca catttcatgc gcttaatttt cagattcgat
     5881 tctgattcca caacttgatc aatacgttca atctgtccat gtgtttcctc gaggtgcgca
     5941 tgaaaagcct gacttaattt ttcgtttgat gttgctctag cgagttttgc cagtgcacgt
     6001 gttaattgtt tttctgcact gtaggtgtct gaaagcaggt gaataaatac atcttcaatg
     6061 gtcttcatat tcatattttt ctccagtgaa atcactgcga gccatacggc ccgcagatgc
     6121 aattatcttt atcagctgat attaataatg aacggtgaat caggattttc ctgatttatt
     6181 accaccgctt tgttgaccgc cttttttacc tgcttcagat gcgcgttgcg gatcattttt
     6241 aaaattaccg ccgctatgct gaccgccttt acggcctgcg tcggatgcct tctcacggtc
     6301 ttcggcgaaa tttcctgaac caccacgatg ttcggccatg ttatttctcc cgttgcgctg
     6361 cattgtttga ttaatatgag tgttgtgtgt cgacactcat taaaattagt cgcaaatgag
     6421 aattagtcaa attaagcgta acgagaaaaa agaggaaaaa tatattttga ggaacattct
     6481 ggatatatta acaattgcct gaggaataag tgacttagag aaaattattg actttactgg
     6541 tgttatgttg cgggattaat ttgtttgatt atattactgt tgggcggaaa aaagacgtaa
     6601 gttgacgttc gacaggggta agcgaaacgg taaaaagata aataataaat gaatttagga
     6661 tttttccggc ttcgttaaag aaagtaaaaa tgccgccagc ggaactggcg gctgtgggat
     6721 taactgcgcg tcgccgcttt catcggttgt acaaaagctt tcagtgccgc cagcattttc
     6781 tctggctcat taatatgttg ctcgatgatt ttaacgatgg ccgaaccaga aatcgcgccc
     6841 gcagctcctg catcaatcgc tgcttttacc tgatccgggg cggaaatacc aaatccctgc
     6901 aatggaggcg cagcattgta ttctttcagc ttcgtgacca gatgattgag gggtaacgcg
     6961 gcgcggtttt ctgcgccggt cacgcccgca cgcgacaata aataggtgta accacgaccg
     7021 taagaggcta tctggcgcag caggtcgtca tcggcatttg gcgggcagat aaagataggt
     7081 gcgacattat gacgcaacgc ggcctggcgg aagggcgcgg actcttccac gggcacatcg
     7141 gcaaccagca ccgaatcgac gccggctttt tcgcactggg cataaaactc atcaatgcct
     7201 ttgttaaaca ccaggttggc atacatcagc aggccaatgg gaatggtcgg gtgtttctgg
     7261 cgaatgagtg ccagcatctc aaagcactgc gccggggtca cacctgccgc aaaggcacgc
     7321 agtgtggcgt tttgaatcgt cgggccatct gccagtgggt cggagaaggg gatgcctaac
     7381 tccagcgcgt cagcaccggc ttcaattagc gcatcgataa ttttcagcga ctgctcaatg
     7441 cccggatcac cgagggtgac gaaaggaacg aatgcgcctt ctttgcgctc cttcaaacgg
     7501 gcaaacagag attcgtagcg ttccatcaga tttcccctcg tgctttcaaa atatcgtgaa
     7561 cggtgaagat gtctttatcg ccgcgaccgg aaaggttaac caccaatagc tgctcttttt
     7621 ccggattttc gcgcatcatt ttcagcgcat gggccagggc gtgggaggat tccagcgccg
     7681 ggatgatccc ttcatgcagg caaagcgttt taaaggcttc cagggcttca tcgtcggtaa
     7741 tagacacgta atcagcgcgt ccagtgctgt tgagatacgc atgttgcggg ccgacggacg
     7801 ggaaatccag cccggcagaa atggagtaag actcttcaat ttgcccgtct tcggtttgca
     7861 tcatcggcgc tttcataccg aaatagatgc ccacgcgacc atgttttaac ggtgcgccgt
     7921 gctcgccagt ttcgataccg tggccgccag gctccacacc aatcaggccg acgtcggttt
     7981 cgttgatgaa atctgcaaac atgccgatgg cattcgaacc gccgccaaca caggcgataa
     8041 ccgcatccgg caggcgacct tctctttcca gaatctgtgc tttggtttct tcgccaatca
     8101 ttcgctgaaa ctcacggaca atggtcggat aaggatgtgg gccagctgcg gtgcccagca
     8161 tatagtgcgc ggtttcataa ctgccggacc agtcgcgcag cgcctcgtta caggcatctt
     8221 tcagcgtcgc ggaaccgcta tgtaccggga tcacttccgc acccattaag cgcatacgaa
     8281 aaacgtttgg cgactggcgt tcaacgtctt tggcacccat ataaatacgg catttcaggc
     8341 cgagcagggc gctggcaagg gccgacgcca cgccatgctg accggctccg gtttcggcga
     8401 tgatttccgt tttacccatc cgcttcgcca gcaacgcctg tcccagcacc tggttagttt
     8461 tatgcgcgcc gccgtgcagc aaatcttcgc gcttcagata cagcgtggtg ttcgtcccgg
     8521 ctgtaatgtt ctggcatttg gtcagcgcgg ttggacgccc ggcatagttt ttcagcaggt
     8581 cgttgaactg agcctgaaat tcaggatctt tttgcgcact gacaaaagct tcttccagct
     8641 ggcgcagagc aggcatcagg atttgtggca cgtacatgcc gccaaactca ccaaaatacg
     8701 ggttaagtaa tgttgtcatt gttcctttcc ttaatatgcg cgcagcgtct ggaaaaccga
     8761 ggccaaaaga cgtgcgtctt tgatgcccgg ttgcgactct acagcagaat taaaatcaag
     8821 tccggcgcag ccggtttgtg ccgcttccac gcagttatct gcgcctaagc cccccgccag
     8881 cagaacgttg ccaagcgatt gaccatttaa tagtgaccag tcgaaacgtt gtccgctccc
     8941 gccctgaccg ttgtcgaata catatttatc gatgtgctga aaatcgcgcg cgggaagagt
     9001 ttcaccgaca cttaaagcct tccagatggc gacgtgtgct ggcagagcct cacgcagagt
     9061 gtcgatatac agctgatctt c
//
LOCUS       DAMZFT010000187         8945 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.187, whole genome shotgun sequence.
ACCESSION   DAMZFT010000187 DAMZFT010000000
VERSION     DAMZFT010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8945)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8945)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8945
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.187"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            237..2048
                     /gene="edd"
                     /locus_tag="PN977_004618"
     CDS             237..2048
                     /gene="edd"
                     /locus_tag="PN977_004618"
                     /EC_number="4.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydratase"
                     /protein_id="HDI6044557.1"
                     /translation="MNPQLLRVTNRIIERSRETRSAYLARIEQAKTSTVHRSQLACGN
                     LAHGFAACQPEDKASLKSMLRNNIAIITSYNDMLSAHQPYEHYPEIIRKALHEANAVG
                     QVAGGVPAMCDGVTQGQDGMELSLLSREVIAMSAAVGLSHNMFDGALFLGVCDKIVPG
                     LTMAALSFGHLPAVFVPSGPMASGLPNKEKVRIRQLYAEGKVDRMALLESEAASYHAP
                     GTCTFYGTANTNQMVVEFMGMQLPGSSFVHPDSPLRDALTAAAARQVTRMTGNGNEWM
                     PIGKMIDEKVVVNGIVALLATGGSTNHTMHLVAMARAAGIQINWDDFSDLSDVVPLMA
                     RLYPNGPADINHFQAAGGVPVLVRELLKAGLLHEDVNTVAGFGLSRYTLEPWLNNGEL
                     DWREGAEKSLDSNVIASFEQPFSHHGGTKVLSGNLGRAVMKTSAVPVENQVIEAPAVV
                     FESQHDVMPAFEAGLLDRDCVVVVRHQGPKANGMPELHKLMPPLGVLLDRCFKIALVT
                     DGRLSGASGKVPSAIHVTPEAYDGGLLAKVRDGDIIRVNGQTGELTLLVDEAELAARE
                     PHIPDLSASRVGTGRELFSALREKLSGAEQGATCITF"
     gene            2085..2726
                     /gene="kdgA"
                     /locus_tag="PN977_004619"
                     /gene_synonym="eda"
     CDS             2085..2726
                     /gene="kdgA"
                     /locus_tag="PN977_004619"
                     /gene_synonym="eda"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HDI6044558.1"
                     /translation="MKNWKTSAESILTTGPVVPVIVVKKLEHAVPMAKALVAGGVRVL
                     EVTLRTECAVDAIRAIAKEVPEAIVGAGTVLNPQQLAEVTEAGAQFAISPGLTEPLLK
                     AATEGTIPLIPGISTVSELMLGMDYGLKEFKFFPAEANGGVKALQAIAGPFSQVRFCP
                     TGGISPANYRDYLALKSVLCIGGSWLVPADALEAGDYDRITKLAREAVEGAKL"
     gene            complement(2782..3960)
                     /gene="purT"
                     /locus_tag="PN977_004620"
     CDS             complement(2782..3960)
                     /gene="purT"
                     /locus_tag="PN977_004620"
                     /EC_number="2.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent phosphoribosylglycinamide
                     formyltransferase"
                     /protein_id="HDI6044559.1"
                     /translation="MTLLGTALRPAATRVMLLGSGELGKEVAIECQRLGVEVIAVDRY
                     ADAPAMHVAHRSHVINMLDGDALRRVVELEKPHYIVPEIEAIATDMLIQLEEEGLNVV
                     PCARATKLTMNREGIRRLAAEELQLPTSTYRFADSENLFREAVADIGYPCIVKPVMSS
                     SGKGQTFIRSAEQLAQAWEYAQQGGRAGAGRVIVEGVVKFDFEITLLTVSAVDGVHFC
                     APVGHRQEDGDYRESWQPQQMSPLALERAQEIARKVVLALGGYGLFGVELFVCGDEVI
                     FSEVSPRPHDTGMVTLISQDLSEFALHVRAFLGLPVGGIRQYGPAASAVILPQLTSQN
                     VTFDNVQNAVGADLQIRLFGKPEIDGSRRLGVALATAESVVDAIERAKHAAGQVKVQG
                     "
     gene            4094..4384
                     /gene="yebG"
                     /locus_tag="PN977_004621"
     CDS             4094..4384
                     /gene="yebG"
                     /locus_tag="PN977_004621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein YebG"
                     /protein_id="HDI6044560.1"
                     /translation="MAVEVKYVVIREGEEKMSFTSKKEADAYDKMLDTADLLDTWLTN
                     SPVQMEDEQREALSLWLAEQKDVLSTILKTGKLPSPQVVGAESEEEDASHAA"
     gene            4451..4807
                     /locus_tag="PN977_004622"
     CDS             4451..4807
                     /locus_tag="PN977_004622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YebF"
                     /protein_id="HDI6044561.1"
                     /translation="MKKRGAFLGLLLVSACASVFAANNETSKSVTFPKCEGLDAAGIA
                     ASVKRDYQQNRVARWADDQKIVGQADPVAWVSLQDIQGKDDKWSVPLTVRGKSADIHY
                     QVSVDCKAGMAEYQRR"
     gene            5124..5783
                     /locus_tag="PN977_004623"
     CDS             5124..5783
                     /locus_tag="PN977_004623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HDI6044562.1"
                     /translation="MANWLNQLQSLLGQSRSSTSSSADQGLGKLLVPGALGGLAGLLV
                     ANKSARKLLTKYGTNALLVGGGAVAGTVLWNKYKDKIRAAHQDEPQFGAQSTPLDERT
                     ERLILALVFAAKSDGHIDARERAAIDQQLREAGVEEQGRVLIEQAIEQPLDPQRLATG
                     VRNEEEALEIYFLSCAAIDIDHFMERSYLNALGDALKIPQDVREGIERDLEQQKRTLA
                     E"
     gene            5990..8050
                     /gene="ptrB"
                     /locus_tag="PN977_004624"
     CDS             5990..8050
                     /gene="ptrB"
                     /locus_tag="PN977_004624"
                     /EC_number="3.4.21.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase B"
                     /protein_id="HDI6044563.1"
                     /translation="MLPKAARIPHAMTLHGDTRIDNYYWLRDDTRSQPKVLDYLQQEN
                     SYGHRVMASQQALQDRILKEIIDRIPQREVSAPYIKNGYRYRQIYEPGCEYAIYQRQS
                     AFSEEWDEWETLLDANKRAAHSEFYSMGGMAITPDNTIMALAEDFLSRRQYGIRFRNL
                     ETGNWYPELLDNVEPSFVWANDSWTFYYVRKHPVTLLPYQVWRHAIGTPASQDTLIYE
                     EKDDTYYVSLHKTTSKHYVVIHLASATTSEVRLLDAELADAEPFVFLPRRKDHEYSLD
                     HYQHRFYLRSNRHGKNFGLYRTRMRDEQQWEELIPPRDNIMLEGFTLFTDWLVVEERQ
                     RGLTSLRQINRKTREVIGIAFDDPAYVTWIAYNPEPETARLRYGYSSMTTPDTLFELD
                     MDTGERRVLKQTEVPGFDAANYRSEHLWIVARDGVEVPVSLVYHRKHFRKGHNPLLVY
                     GYGSYGASIDADFSFSRLSLLDRGFVYAIVHVRGGGELGQQWYEDGKFLKKKNTFNDY
                     LDACDALLKLGYGSPSLCYAMGGSAGGMLMGVAINQRPELFHGVIAQVPFVDVVTTML
                     DESIPLTTGEFEEWGNPQDPQYYEYMKSYSPYDNVTAQAYPHLLVTTGLHDSQVQYWE
                     PAKWVAKLRELKTDNHLLLLCTDMDSGHGGKSGRFKSYEGVAMEYAFLVALAQGTLPA
                     TPED"
     gene            complement(8047..8709)
                     /gene="exoX"
                     /locus_tag="PN977_004625"
     CDS             complement(8047..8709)
                     /gene="exoX"
                     /locus_tag="PN977_004625"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease X"
                     /protein_id="HDI6044564.1"
                     /translation="MLRIIDTETCGLQGGIVEIASVDVIDGKIVNPMSHLVRPDRPIS
                     PQAMAIHRITEAMVADKPWIEDVIPHYYGSEWYVAHNASFDRRVLPEMPGEWICTMKL
                     ARRLWPGIKYSNMALYKTRKLNVQTPPGLHHHRALYDCYITAALLIDIMNTSGWTAEQ
                     MADITGRPSLMTTFTFGKYRGKAVSDVAERDPGYLRWLFNNLDSMSPELRLTLKHYLE
                     NT"
     gene            complement(8733..>8945)
                     /gene="yobB"
                     /locus_tag="PN977_004626"
     CDS             complement(8733..>8945)
                     /gene="yobB"
                     /locus_tag="PN977_004626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-N hydrolase family protein YobB"
                     /protein_id="HDI6044565.1"
                     /translation="LGEPDLLASARRLQFFSHQYSIAVLMANARGNSALWDEHGRLIV
                     RADRGSLLLVGQRSSQGWQGDIIPLR"
BASE COUNT         2211 a         2249 c         2361 g         2124 t
ORIGIN      
        1 aatatctgcg ctcatccttt atggttattt taccggtaac atgatcttgc acagattgta
       61 gaacaatttt tacactttca ggcctcgtgc ggattcaccc acgaggcttt ttttattaca
      121 ctgactgaaa cgtttttgcc ctatgagctc cggttacagg cgtttcagtc ataaatcctc
      181 tgaatgaaac gcgttgtgaa tcatcctgct ctgacaactc aatttcagga gcctttatga
      241 acccacaatt gttacgcgta acaaatcgaa tcattgaacg ttcgcgcgag actcgctctg
      301 cttatctcgc ccggatagaa caagcgaaaa cttcgaccgt tcatcgttcg cagttggcat
      361 gcggtaacct ggcacacggt ttcgctgcct gccagccaga agacaaagcc tctttgaaaa
      421 gcatgttgcg taacaatatc gccatcatca cctcctataa cgacatgctc tccgcgcacc
      481 agccttatga acactatcca gaaatcattc gtaaagccct gcatgaagca aatgcggttg
      541 gtcaggttgc gggcggtgtt ccggcgatgt gtgatggtgt cacccagggg caggatggaa
      601 tggaattgtc gctgttaagc cgcgaagtga ttgcgatgtc tgcggcggtg gggctgtccc
      661 ataacatgtt tgatggtgcg ctgttcctcg gtgtgtgcga caagattgtt ccgggtctga
      721 ctatggcagc cctgtcgttt gggcatttgc ccgcggtgtt tgtgccgtct ggaccgatgg
      781 caagcggttt gccaaataaa gaaaaagtgc gtattcgtca gctttatgcc gaaggtaaag
      841 tggaccgcat ggcactactg gagtcagaag ccgcatctta tcatgcgccg ggaacatgta
      901 ctttctacgg tactgccaac accaaccaga tggtggtgga gtttatgggg atgcagttgc
      961 caggctcttc atttgttcat ccggattctc cgctgcgcga tgctttgacc gcagccgctg
     1021 cgcgccaggt tacacgcatg accggtaatg gtaatgaatg gatgccgatc ggtaagatga
     1081 tagatgagaa agtggtggtg aacggtatcg ttgcactgct ggcgaccggt ggttccacta
     1141 accacaccat gcacctggtg gcgatggcac gcgcggccgg tattcagatt aactgggatg
     1201 acttctctga cctttctgat gttgtaccgc tgatggcacg tctctacccg aacggtccgg
     1261 ctgatattaa ccacttccag gcggcaggtg gcgtaccggt tctggtgcgt gaactgctca
     1321 aagcaggcct gctgcatgaa gatgtcaata cggtggcagg ctttggtctg tctcgttata
     1381 cccttgaacc atggctgaat aatggtgaac tggactggcg ggaaggggcg gaaaaatcac
     1441 tcgacagcaa tgtgatcgct tccttcgaac aacctttctc tcatcatggt gggacaaaag
     1501 tgttaagcgg taacctgggc cgtgcggtta tgaaaacctc tgccgtgccg gtagagaacc
     1561 aggtgattga agcgccagcg gttgtttttg aaagccagca tgacgttatg ccggcctttg
     1621 aagcgggttt gctggaccgc gattgtgtcg ttgttgtccg tcatcagggg ccaaaagcga
     1681 acggaatgcc agaattacat aaactcatgc cgccacttgg tgtattattg gaccggtgtt
     1741 tcaaaattgc gttagttacc gatggacgac tctccggcgc ttcaggtaaa gtgccgtcag
     1801 ctatccacgt aacaccagag gcctacgatg gcgggctgct ggcaaaagtg cgcgacgggg
     1861 acatcattcg tgtgaatgga cagacaggcg aactgacgct gctggtagac gaagcggaac
     1921 tggctgctcg cgaaccgcac attcctgacc tgagcgcgtc acgcgtggga acaggacgtg
     1981 aattattcag cgccttgcgt gaaaaactgt ccggtgccga acagggcgca acctgtatca
     2041 ctttttaaga cgacaaattt gtaatcaggc gagagaaaac tctgatgaaa aactggaaaa
     2101 caagtgcaga atcaatcctg accaccggcc cggttgtacc ggttatcgtg gtaaaaaaac
     2161 tggaacacgc ggtgccgatg gcaaaagcgt tggttgctgg tggggtgcgc gttctggaag
     2221 tgactctgcg taccgagtgc gcagttgacg ctatccgagc tatcgccaaa gaagtgcctg
     2281 aagcgattgt gggtgccggt acggtgctga atccacagca gctggcagaa gtcacagaag
     2341 cgggtgcaca gtttgcaatt agcccgggtc tgaccgagcc gctgctgaaa gctgctaccg
     2401 aagggactat tcctctgatt ccggggatca gcactgtttc cgaactgatg ctgggtatgg
     2461 actacggttt gaaagagttc aaattcttcc cggctgaagc taacggcggt gtgaaagccc
     2521 tgcaggcgat cgcgggtccg ttctcccagg tccgtttctg cccgacaggt ggtatttctc
     2581 cggctaacta ccgtgactac ctggcgctga aaagcgtgct gtgcatcggt ggttcctggc
     2641 tggttccggc agatgcgctg gaagcgggcg attacgaccg cattactaag ctggcgcgtg
     2701 aagctgtaga aggcgctaag ctgtaaaagt caaaatgccc gatcaaggat cgggcatttt
     2761 tgtagcggtt ttttgccggg tttaaccctg tacttttacc tgtccggcgg cgtgcttcgc
     2821 gcgttcaatg gcgtcaacaa cactctctgc agtagccagt gctaccccca gacgacggct
     2881 gccatcaatt tccggcttac caaataaacg aatctgtaaa tctgcgccta cggcattctg
     2941 cacattatca aacgtgacat tctgactggt cagttgcggc aggataacgg cagaagcagc
     3001 aggaccatac tggcggatcc cgccaaccgg aagtccgagg aaggcacgta catgcagggc
     3061 aaactccgag agatcctgag aaattagcgt caccatcccg gtgtcatgcg ggcgagggga
     3121 gacttcactg aaaatcacct catcaccaca gacaaacagc tcgacaccaa acagcccata
     3181 accgcccagt gccagcacca ctttacgggc aatctcctgc gcacgttcaa gggcaagcgg
     3241 gctcatttgt tgtggttgcc aggattcacg atagtcgcca tcttcctggc gatgacctac
     3301 cggtgcacag aaatggacgc catccaccgc gctgacggtt agcagggtaa tttcgaaatc
     3361 aaacttaacg acgccttcaa caattacacg gcccgctccg gcgcgaccgc cttgctgagc
     3421 gtactcccat gcctgagcaa gttgctctgc agaacgaata aacgtctgcc ccttgccgga
     3481 ggaactcatc accggtttaa caatgcaggg atagccgatg tcagcaaccg cctcgcggaa
     3541 aaggttttcg ctatcggcaa aacgataagt ggaagtgggc agctgcagct cttctgccgc
     3601 caggcgacgg ataccctcgc gattcatcgt taatttcgtt gcgcgcgcgc aggggacaac
     3661 attcagtcct tcctcttcaa gttggatcag catatcggtg gcaatagctt cgatctccgg
     3721 cacgatataa tgtggttttt ccagttcaac cacacggcgt aatgcatcac catcaagcat
     3781 attaatgaca tgggagcgat gcgcgacatg catagctggc gcgtcggcat agcgatcgac
     3841 ggcaatcacc tctacgccga gacgctgaca ctcgattgcc acttctttac ccagttcacc
     3901 ggagcctaat aacatcacgc gagttgctgc cggacgcagc gcagtgccta ataacgtcat
     3961 aacgaatatc ccctgattaa ttccgcgcgc agtataaacg aaaacgtttg cgcgtgtctt
     4021 tattgttgat gttgatttcc caaccgagaa gaaatatact gtataaaatc acagttatta
     4081 tgagaggctg caaatggcgg ttgaagttaa atacgtagtc attcgtgagg gcgaagagaa
     4141 aatgtcgttt accagcaaaa aggaagccga tgcttatgac aaaatgcttg atacggcgga
     4201 tctgcttgac acctggctga caaattctcc agtgcaaatg gaagacgagc aacgtgaagc
     4261 cctttcgcta tggctggcag aacaaaaaga tgtgctgagc accattctga aaaccggcaa
     4321 attaccgtcc ccacaggtcg tcggtgctga atccgaagag gaagatgcct cacacgcggc
     4381 ctgaagcaac ttgcgctcct gacaatttgt accatgcttt ctcctgaatc attgtgctat
     4441 ggagaaaaac atgaaaaaaa gaggggcgtt tttagggctg ttgttggttt ctgcctgcgc
     4501 atcagttttc gctgccaata atgaaaccag caagtcggtc actttcccaa agtgtgaagg
     4561 tctggatgct gccggaattg ccgcgagcgt aaaacgtgat taccaacaaa atcgcgtggc
     4621 gcgctgggct gatgatcaaa aaattgtcgg tcaggccgac cccgtggcat gggtcagttt
     4681 gcaggacatt caggggaaag atgataaatg gtcagtaccg ctaaccgtgc gtggtaaaag
     4741 tgccgatatt cattaccagg tcagcgtgga ctgcaaagcg ggaatggcgg aatatcagcg
     4801 gcgttaatgg cttctgataa ttcgattgac cgttggcgga tgcggcgcga atgccttgtc
     4861 cgccccacaa tcgtaggcct gagaagacgc aacagcgtcg catcgggcaa tcagcatcaa
     4921 acagtgctac tcaaaacgtc aaactacttt cacctaaaat acccttatca cctgatatac
     4981 agtgaacacc ttacctggcg cgaatttctt tgaataacgg cctaccgact cgcttatttg
     5041 cgcatctttt gtctcgatat acattttctg acatcctcat ccgctatgct acaggcacta
     5101 aaaatggtta ttgaggagca aatatggcta actggttaaa tcaactgcaa tccctgcttg
     5161 ggcaaagccg ttcttctacc tcctcgtctg cggatcaggg attgggcaaa cttttagtgc
     5221 caggcgcatt aggcggactg gctgggctgc tggtcgcaaa taaatcagca cggaaacttc
     5281 ttacgaaata tggcaccaac gcgttactgg ttggcggcgg agcggtcgcg ggtacggtgc
     5341 tgtggaataa atacaaagat aaaattcgcg cggcgcatca ggacgaaccg cagtttggcg
     5401 cgcaaagtac gccgctggat gagcgtacgg aacgtttgat ccttgcgctg gtctttgccg
     5461 ctaaaagtga tggtcatatt gatgccagag aacgtgcggc aatcgaccag caattgcgtg
     5521 aagccggcgt ggaagagcag gggcgtgtac tcattgagca ggcaatcgaa caaccgctgg
     5581 atccacaacg cctggctacc ggggtccgca atgaagaaga ggcgctggaa atctatttcc
     5641 tgagctgcgc ggctattgat attgaccatt ttatggaacg cagttacctg aatgcactgg
     5701 gtgacgcgct gaaaattccc caggacgttc gtgaaggcat tgaacgcgat ctcgaacaac
     5761 aaaaacgcac tttggcggaa taattccgct ctggatagcc tgaagttgac gatttcgggc
     5821 tatccagaac cgcacggcca tttgagcagg ctgattttct ctgtctgcat aacattcgat
     5881 atccgcgcca ttacctgtca tcatctaagc aatgactacc ctgtttcgct tgcatccccg
     5941 gtgagttttg ccacccttat aagatgtttc aaccagaaag aacaataaca tgctaccaaa
     6001 agccgcccgc attccccacg ccatgacgct tcatggcgat acgcgcatcg ataattacta
     6061 ctggctgcgg gacgatacgc gttctcagcc gaaagtcctg gactacctgc agcaagaaaa
     6121 tagttacggt catcgggtga tggcctcgca acaagccttg caggatcgca tcttaaagga
     6181 aatcatcgac cgcattccgc aaagagaagt ttctgcgccc tacataaaaa atggctaccg
     6241 ctatcggcag atttatgaac caggctgtga atatgctatc taccagcgtc aatcggcatt
     6301 cagtgaagag tgggatgagt gggaaacatt gctcgatgcc aataagcgcg cggcgcacag
     6361 tgagttttat tcgatgggcg gaatggcgat tacgcccgat aacaccatta tggcgctggc
     6421 agaagatttt ctttcccgac gccagtacgg cattcgtttt cgtaatctgg aaactggtaa
     6481 ctggtacccg gaactgctgg ataacgttga acccagcttt gtctgggcaa atgactcctg
     6541 gactttctac tatgtacgca agcatccagt tacgctgctg ccttatcagg tctggcgtca
     6601 cgccatcggt acgccagcat cgcaagatac actgatctac gaagaaaaag acgataccta
     6661 ttacgtcagc ctgcataaaa cgacctcgaa gcactatgta gtcattcatc tggccagcgc
     6721 caccaccagt gaagttcgcc tgctggacgc ggaattggcc gatgccgagc cgtttgtttt
     6781 tctgccgcgc cgcaaagatc acgaatacag ccttgatcac taccagcatc gtttttatct
     6841 gcgttccaac cgccacggca aaaactttgg cttataccgt acccgtatgc gtgatgagca
     6901 acagtgggaa gagttaattc cgccacgtga taacattatg ctggaagggt ttacgctgtt
     6961 taccgactgg ctggtggttg aagagcgtca gcgcgggtta accagtttgc ggcaaattaa
     7021 ccgcaagacc cgggaagtca ttggcattgc ctttgatgat ccggcctatg tgacctggat
     7081 tgcctacaat ccagaacctg aaaccgcgcg attgcgttat ggttattctt ccatgaccac
     7141 accagacact ctgtttgaac tggatatgga taccggtgag cgtcgtgtat taaaacaaac
     7201 ggaagttcct ggttttgatg cggcgaatta ccgcagtgaa cacctgtgga tagtcgcccg
     7261 ggatggcgtc gaagttccgg tttcgttggt ctaccatcgc aaacattttc gcaaaggaca
     7321 caacccgctg ctggtgtatg gctacggttc ttacggcgca agtattgatg ccgatttcag
     7381 ttttagccgc ttgagtttgt tagatcgtgg ctttgtctac gccattgtcc atgttcgcgg
     7441 cggtggtgag ctggggcaac aatggtacga agacggaaaa ttcctgaaga agaaaaatac
     7501 gtttaatgat tatcttgatg cctgcgatgc attgttaaaa ctgggctatg gatcaccttc
     7561 gctctgttat gcgatgggcg ggagtgccgg gggcatgttg atgggcgttg caattaatca
     7621 acgcccggaa ttattccacg gcgttatcgc ccaggtaccg tttgttgatg ttgtaacaac
     7681 gatgcttgat gaatcaattc ctcttaccac tggtgagttt gaagagtggg ggaatccgca
     7741 ggatccgcaa tattacgagt acatgaaaag ctacagcccg tatgacaacg tcaccgcaca
     7801 ggcttatccg catttactgg taacgaccgg tttacacgat tctcaggtgc aatattggga
     7861 accggcaaaa tgggtggcta aattgcgcga gctgaaaacc gataaccatc ttttattgct
     7921 ctgtaccgac atggactcag gccatggcgg taaatctggc cgctttaaat cgtacgaagg
     7981 cgtagcgatg gaatatgctt ttctggtcgc gctggcgcag ggaacattac ccgctacgcc
     8041 tgaggattaa gtattttcca gataatgttt cagtgttaaa cgcagctccg ggctcatgct
     8101 gtccaggtta ttaaacaacc agcgcagata gcccggatcg cgttcggcaa cgtcggaaac
     8161 cgctttgcca cggtatttgc caaaggtgaa ggtcgtcatc aacgacggac gtccggtgat
     8221 atcggccatc tgttctgccg tccagccgga ggtgttcata atatcgataa gcaacgcagc
     8281 ggtgatataa cagtcataca acgcgcggtg atgatgcagg cccggcggcg tctgtacatt
     8341 gagcttccgt gttttatata acgccatatt gctatacttg atccctggcc acaaacgacg
     8401 ggccagtttc atagtgcaaa tccactcacc gggcatctca ggcagtacgc ggcggtcaaa
     8461 gctggcgttg tgcgcgacat accattcact accgtaatag tgcgggatca catcttcaat
     8521 ccacggttta tcggcgacca tggcttcggt gatgcgatga atcgccatcg cttgtggact
     8581 aataggacga tcggggcgca ccaggtggct catggggttg acgatttttc cgtcaatgac
     8641 atcaacagag gcaatctcaa cgatccctcc ctgcaaaccg caggtttctg tatcgataat
     8701 gcgcaacatg gcatgctcca ggccgaaaaa gcctagcgta atggaatgat atcgccttgc
     8761 caaccctgtg atgaacgctg accgactaac aataatgaac cacgatcggc gcgaacgatg
     8821 agacgaccat gttcatccca tagtgcactg ttaccacggg catttgccat cagcacagca
     8881 atcgagtact gatgtgaaaa aaattgtaag cgacgggccg acgccagcag gtcgggttct
     8941 cccag
//
LOCUS       DAMZFT010000188         8824 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.188, whole genome shotgun sequence.
ACCESSION   DAMZFT010000188 DAMZFT010000000
VERSION     DAMZFT010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8824)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8824)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8824
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.188"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            294..455
                     /locus_tag="PN977_004627"
     CDS             294..455
                     /locus_tag="PN977_004627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001571575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6044566.1"
                     /translation="MTTQNNKHDSLDYSEKPKSKHIVTFERDSGRKGESSSSANKTTM
                     PAAPAPGKK"
     gene            complement(459..1091)
                     /locus_tag="PN977_004628"
     CDS             complement(459..1091)
                     /locus_tag="PN977_004628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000460689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6044567.1"
                     /translation="MFDVSLLNLPWATLVTLTSGYIGYFIANVGLKDHHKPIEVTFSS
                     LIFGLTAMMAYQAVMWAGLNAWLATPPALLCAVTCGAWWRRYGRKWMYRLLWNNDISW
                     SDDTSSAWQAMFDQTGFSVTEVRVILRDGSGMMSRLPGNFEEWPNGPFTLGNKGDMVL
                     YVTHSSPSGSNEWEEYKGVVDKYWGALATYIPADQIARVEIRRVRATNDE"
     gene            complement(1206..1604)
                     /locus_tag="PN977_004629"
     CDS             complement(1206..1604)
                     /locus_tag="PN977_004629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001576704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HDI6044568.1"
                     /translation="MSDLNQFRSKGPELLVELAQHTSETVREIIDIEPAIADQIGQAV
                     ANRMMQVWGGQNVYFPMGMVWKVSQRDREIFREFNGRNHHELARKFGVSLQWVYSVVK
                     RVRKEELDRMQGRLFDEDLPEETKDVNKSR"
     gene            complement(1576..2025)
                     /locus_tag="PN977_004630"
     CDS             complement(1576..2025)
                     /locus_tag="PN977_004630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001610542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory protein GemA"
                     /protein_id="HDI6044569.1"
                     /translation="MTKQRLIQLIHIARNELGMDEDTYRQMLQGLTGKASTKGMDTTQ
                     LNCVLESMKRKGFRVKPAGKASSGLPLDNHPQSRKIRALWLEMAAAGIVRDRSENALA
                     RWIKRETGISALRWLNTEQASSVIEKLKKWQHRAAGVKHERPESVSK"
     gene            complement(2018..2233)
                     /locus_tag="PN977_004631"
     CDS             complement(2018..2233)
                     /locus_tag="PN977_004631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6044570.1"
                     /translation="MTAESVVCALFWYCFVGWCTAELHRRSGFYSRYSGAGYWISWSV
                     MFLCWPVALPLYVDYIGGAGKRSNDDD"
     gene            complement(2223..2453)
                     /locus_tag="PN977_004632"
     CDS             complement(2223..2453)
                     /locus_tag="PN977_004632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000631819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6044571.1"
                     /translation="MITLSGNSRKLKACRISARYLFARAFFKNVRPGITIGVIAGREQ
                     VEKYMSGAWWNNDPVIAARNIHISWGDIQNDG"
     gene            complement(2450..3133)
                     /locus_tag="PN977_004633"
     CDS             complement(2450..3133)
                     /locus_tag="PN977_004633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000131939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2786 domain-containing protein"
                     /protein_id="HDI6044572.1"
                     /translation="MTDQNKHIEKLKKLLALAASGNPHEAALALRRARKLMDVHGITH
                     SDIAMSDIDETISHYWPTGSLRPPRYMPGLMNIIREVFGVNSIIHPGTHPSVGFYGNR
                     ERAALAAYTWEVLARQLKKARQQYISAQNKRIKTATRTSRGDQFAEGWVLAVISEIQS
                     FALTDDERELMQQWLEHKYPQTQTTRARKPGRSRNGDASRYAGFREGQNVRLHRPVSG
                     QEQQKLEAR"
     gene            complement(3130..3345)
                     /locus_tag="PN977_004634"
     CDS             complement(3130..3345)
                     /locus_tag="PN977_004634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000763553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6044573.1"
                     /translation="MADILREITAWILILTGLATCLSAGAALAALLMHITTQWLWEKL
                     KAAYSLKELSDAVRAWKRQKNTGDTEQ"
     gene            complement(3360..3656)
                     /locus_tag="PN977_004635"
     CDS             complement(3360..3656)
                     /locus_tag="PN977_004635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6044574.1"
                     /translation="MTRNTILTRTALYRLALQRFGPDAQALKLTEEAAELAASAARNL
                     NGQGSESDLAAELADVEIMTEQLRLQGMDRLIDFHKQKKLERLAARLGVIYTNE"
     gene            complement(3666..3938)
                     /locus_tag="PN977_004636"
     CDS             complement(3666..3938)
                     /locus_tag="PN977_004636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000632581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6044575.1"
                     /translation="MITPQEARQRTRTLVEHYVNECECRDLTDVKHVLTALISMTTQA
                     IVATNGKAAALQVLMNTLTHTAEHEVPYRMETTAEGGLHITVSRKH"
     gene            complement(4227..4757)
                     /locus_tag="PN977_004637"
     CDS             complement(4227..4757)
                     /locus_tag="PN977_004637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001576700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host-nuclease inhibitor Gam family protein"
                     /protein_id="HDI6044576.1"
                     /translation="MAKSTKGAKRIKAAAALWVPGTREEVIEGIRLLGDAQRELVRAE
                     TEMNDAIGDITARYAPLTESLKKRMAELQSGIQTWCEAHRDELTGNGKVKFANLTTGE
                     VQWRNRPPSVSIRGADNVIELLRRLGLERFIRVKEEINKDAILNEKEAVKNIPGISIK
                     SDIEDFSIIPFEQDVQ"
     gene            complement(4785..5054)
                     /locus_tag="PN977_004638"
     CDS             complement(4785..5054)
                     /locus_tag="PN977_004638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000843445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6044577.1"
                     /translation="MKSTTGINQQISKVQSAIMALKATNTDVQSITIRGNKPVIRVSR
                     SAHCMRMLEQGKACYLYTGHDHRGYFRQGVFELHGCRVVWPESLW"
     gene            complement(5057..6223)
                     /locus_tag="PN977_004639"
     CDS             complement(5057..6223)
                     /locus_tag="PN977_004639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020803496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDI6044578.1"
                     /translation="MLVLKQQLKEARIPQAVAARAVDVSEATLAQIVNHNAWPRTSPG
                     EVRRRLASWLESQGIDTTKSFDAVQGAATPRTAGTTDKTSLSEEENMLLKKQVLFPAT
                     KKAFGLFRDPFADEAMQGSDDVFTTPDIRYVREALYQTARHGGFMAVIGESGAGKSTL
                     RRDLTERINRENAPVIVIEPYIIAMEDNDVKGKTLKAAAIAEAIISTIAPLESIRRSQ
                     DARFRQLHRVLKDSSQAGFSHVLVIEEAHSLPIPTLKHLKRFFELESGFKKLLSIVLI
                     GQPELATKLSERNMEVREVVQRCEVVELLPLDNNLEEFLTFKLQRAGKQLTDIMDASA
                     VDAIRARLSNPGSHRKNMVSLLYPLAVSNLVIAAMNLAAEIGVPQVNADVVKGV"
     gene            complement(6234..8003)
                     /locus_tag="PN977_004640"
     CDS             complement(6234..8003)
                     /locus_tag="PN977_004640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001756569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HDI6044579.1"
                     /translation="MTPALTEKLVETARAARNAGHGKRGAIYDAACAELGMSRATLLR
                     RLKEVSVTDKRKKRADAGRSTLTRDEAALISATLREATRKNGKRLYSIADAVETLRAN
                     GFISAGRTDETTGEFFPLSEDAISRALRNYGLHPEQLDAPAPHTEVASLHPNHVWQID
                     ASLCTLYYLSNGHKGLQVMDSAKFYKNKPANLARIASDRVWSYEITDHASGWIYVEYV
                     TGAESGENLCSVLINAMQERGGADVLHGVPKILYLDPGSANTAGMTKNMCRSLGIDLI
                     AHKPHNARATGQVEKARDIIERKLEPGLKFRPVHSLEELNALAAKWRSHFNATAVHSR
                     HGKTRTDIWLKITAEQLKKAPSVEVCRELAVAAPELRKVTPKLRVSFRGTEFDVSTVP
                     GVLVGEKLMITRNPWRSDVAQVVLTGEDGHETFFLVEEVRKNEFGFAEGAAVFGESYK
                     ALPETPAQMAAKETEALVTGTDNAADAAAARKAKALPFGGRLDPYKHIDDATLPACIP
                     KRGQASDVRGPRTEQRPMTHVEAAKALRDKFSADGLTWTPEHYRQLTAQYPDGVPEAA
                     LDEVMATLTTPARSSVISIVNGN"
     gene            complement(8019..8336)
                     /locus_tag="PN977_004641"
     CDS             complement(8019..8336)
                     /locus_tag="PN977_004641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001095644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6044580.1"
                     /translation="MNTDNTQNILMDSPEALGHALCNLLPEMVQGFRVVTPSGEICVP
                     AQEAHPFVLTMEVMLMQQIRRLQHQSALRPAVAPQPVNNVVKTCDGETLRDRARKIAA
                     RIG"
     gene            complement(8336..>8824)
                     /locus_tag="PN977_004642"
     CDS             complement(8336..>8824)
                     /locus_tag="PN977_004642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001488438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3102 domain-containing protein"
                     /protein_id="HDI6044581.1"
                     /translation="LADGGTVAGLTLDDVDRMSVRELRQALREARETNAAQQQVLAGK
                     DEKINELATKLEKKSRIQPPEPDEEVKKLRAEVTALAVEAESAIAVRLSGAFETLCEY
                     CSKNMIDTPRDFMAGLVCQIERSVHALRETFDLDAAPSGNDVPAWLTEPEPEINRPEG
                     MQ"
BASE COUNT         1854 a         2452 c         2278 g         2240 t
ORIGIN      
        1 gttgcggaag aaccactttt catccgtcgt tgacagatgt gaagtatgcg ggagaaaaag
       61 tgaatatcta actgtaataa ctcagaccgg tacgagttga tcagacatga gataaatgaa
      121 gggggatccg tgagataaat aacgaattgg agtgggttaa catgggttct ggtgaacaga
      181 actgaaattc ttttccgcta tacgatagat aagtcgggca ttgaaatcat ccttcctggc
      241 aacttagaat cgagtttgct gaaacatgac ccgcaacaaa aaggatgatt ccaatgacta
      301 ctcaaaacaa taagcatgat agcctggatt actcagaaaa gcccaaaagc aagcacatcg
      361 tgacgttcga gcgcgattcc ggtagaaaag gagaatcgtc ttcctctgcc aataaaacca
      421 ctatgccggc agcccctgcc ccagggaaaa aataaacatc attcatcgtt cgttgcacga
      481 acgcgcctga tctccactct ggcaatctga tctgctggaa tataggttgc cagagctccc
      541 cagtacttat caaccacgcc tttatactct tcccattcgt tactgcctga agggctgctg
      601 tgcgtgacgt aaagaaccat atcgccttta ttccccaggg taaacggacc gttaggccac
      661 tcttcaaagt tccctggcag ccgtgacatc ataccggaac catcgcgaag aatcactctg
      721 acctcggtaa cgctgaagcc tgtttgatca aacattgcct gccatgctga gctggtatcg
      781 tcagaccatg aaatatcatt attccacagt aatctgtaca tccatttgcg accgtacctg
      841 cgccaccacg caccgcatgt aacggcacac agaagtgctg gtggtgttgc cagccaggca
      901 ttcagacctg cccacataac agcctggtaa gccatcattg ctgtcaggcc aaaaatcagc
      961 gaagaaaaag tcacctcaat gggcttgtgg tgatccttca gtcccacatt cgcaataaaa
     1021 tacccaatat agccactggt cagggtcacc agtgttgccc atggcagatt taacaacgaa
     1081 acgtcaaaca tattttctcc ctgcatcctg aatactaaag agagagcata aaaaaagcac
     1141 ggtaaggggt ctaccgtgct tttatacaat aaggagattt ttgaaaggac tttaacaagc
     1201 aggaattatc tggatttgtt aacgtctttc gtctcctctg gcagatcttc atcaaacaac
     1261 ctgccctgca tccgatccag ttcttctttt ctgacccgct taaccacgct gtagacccac
     1321 tgaagcgaaa caccaaactt gcgggccagt tcgtggtggt tgcgtccgtt aaactccctg
     1381 aagatttccc ggtcgcgctg actgaccttc cataccatgc ccatcgggaa ataaacgttt
     1441 tgcccgcccc agacctgcat cattcggttc gcgacggcct gaccaatctg gtcggcaatt
     1501 gcgggctcaa tatcaataat ctcgcgaacg gtctcagagg tatgctgtgc cagctccacc
     1561 aggagttccg gccctttact tcgaaactga ttcaggtcgc tcatgtttta ctcccgcagc
     1621 tctgtgctgc cacttcttca gtttctcaat aacactgctt gcctgttcag tattgagcca
     1681 gcgcagggcg ctgatgcccg tttcccgctt gatccaccgc gctaatgcat tttctgaacg
     1741 gtcacgaaca atgccggcag cagccatttc aagccatagc gcacggattt tcctggactg
     1801 cggatggtta tccagcggta aaccggagct ggcttttccg gcaggcttaa cgcgaaagcc
     1861 tttccttttc atggattcca gcacgcagtt tagttgtgtg gtatccattc ctttggttga
     1921 ggctttaccg gtcagcccct gtaacatctg gcgataggtg tcttcatcca tacccagttc
     1981 attacgggca atatgaatga gctggataag acgttgttta gtcatcatcg ttgctccttt
     2041 tacctgcgcc accgatataa tcgacatata aaggaagcgc cacaggccag cacaggaaca
     2101 tcaccgacca gctaatccag tagccggcac cactgtaacg tgaataaaac cctgaacggc
     2161 ggtgcagttc agcagtacac caaccgacaa aacaatacca gaacagggca catacaacag
     2221 attcagccgt cattctgaat atccccccaa ctgatatgaa tattacgggc agcaatgaca
     2281 gggtcgttat tccaccatgc acctgacatg tatttttcaa cctgttcgcg tccggcaata
     2341 acaccaattg tgatccccgg cctgacgttc ttaaaaaagg cgcgggcaaa aaggtatctg
     2401 gcagatattc ggcaggcttt taatttccgg ctgttacctg atagcgtaat catctggcct
     2461 ccagtttctg ttgttcctgc ccactgaccg ggcggtgcag tctgacgttc tgcccttcac
     2521 gaaaccccgc atagcgcgag gcgtcgccat tgcggcttct tcccggttta cgcgccctgg
     2581 tggtttgcgt ctgcgggtat ttatgttcca gccactgctg catcagttca cgctcatcat
     2641 cggtcagggc aaaggactgt atttcactga taacggccag cacccagcct tcggcaaact
     2701 ggtctccacg gctggtgcgg gtggcggttt ttattctttt gttctgtgca ctgatatact
     2761 gctgacgcgc ctttttcagc tgacgggcca gcacttccca ggtgtatgca gccagtgccg
     2821 cacgttcccg gttgccgtag aaccccacag acggatgcgt gccggggtga atgatggagt
     2881 taacaccaaa tacctcgcgg atgatgttca tcaggcctgg catgtagcgc ggtggacgga
     2941 gactgcctgt cggccagtag tgactgatgg tttcatcaat atcactcata gcaatgtcgg
     3001 aatgtgtgat gccgtgaaca tccatcagtt tacgggctcg gcgcagtgcc agagcggcct
     3061 cgtgcgggtt gccggatgcg gccagcgcca gcaacttttt cagtttctca atgtgtttat
     3121 tctggtctgt cattgttctg tatctccggt atttttctgc cgtttccatg cccggacagc
     3181 gtcggacagc tctttcaggc tgtatgctgc tttaagcttt tcccataacc actgtgttgt
     3241 tatgtgcatc agcagggcag ccagggctgc ccccgcactg aggcatgtcg ccaggccagt
     3301 aagaatcagt atccatgcgg tgatttccct gagtatgtca gccattgaat gcctcccggt
     3361 tactcgttcg tgtaaatcac gcccagtcgt gcagccagac gttccagttt tttctgtttg
     3421 tggaagtcaa tcagccggtc catcccctga aggcgcaatt gctctgtcat gatttccacg
     3481 tctgccagct ctgccgcgag gtcgctttcg ctgccctgtc cgttcagatt gcgggcagca
     3541 ctggccgcca gttcagcggc ctcttctgtc agtttcaggg cctgtgcgtc cggcccgaaa
     3601 cgctgcaggg ccagacggta gagggcggtg cgggtgagta tggtgttccg tgtcatgccg
     3661 cgccctcagt gcttccggct gacggtgatg tgcaggccgc cttctgcagt ggtttccatc
     3721 cggtacggca cctcatgctc tgccgtgtgg gtgagtgtgt tcatcagtac ctgcagggca
     3781 gccgcctttc cgttggttgc cacaatggcc tgtgtggtca tgctgattag cgccgtcagg
     3841 acgtgcttca catcggtgag gtcgcggcat tcacactcgt tgacatagtg ttcaacaagg
     3901 gttcgggtgc gctgtcgtgc ttcctgtggg gtaatcattg cgagtcctcc ctgtcagggc
     3961 gggagaattc catgacgggc acgtctgccg aaaaatgctg gctgcagtac gggcagacca
     4021 gggtgacgcg taccgcaggg atgtggtatt taccggacat cacggcgatg gcgctgtgaa
     4081 aacgcagggc tgttatatcc ctctcgcact gaatacattt aaatatcatg atttaattct
     4141 cctctgtttc cggcgtgcag aagcccgcgg cgctgacgcc ggaataaaaa agaaaatgtt
     4201 tttattaaat aattaacgtg gtgtgtttac tgcacatcct gctcaaaagg aattattgaa
     4261 aaatcctcaa tgtcgctttt aatggaaata ccgggaatat ttttcacggc ctctttttca
     4321 ttcaggatag cgtctttatt tatttcctct tttacacgaa taaagcgctc aagccccaga
     4381 cgtctcagta attcaataac attatccgcc ccacggatac tgactgatgg cggacggttt
     4441 cgccactgca cctcgccggt ggtaaggtta gcgaacttca ccttcccgtt gccggtcagt
     4501 tcatcacggt gcgcctcaca ccatgtctgg ataccggact gcagttcggc catgcgtttt
     4561 ttcaggctct cggtgagcgg ggcataacgt gcggtgatat cgccaatggc gtcattcatt
     4621 tctgtttcag ccctgaccag ttcacgttgt gcgtcaccga gtagtctgat tccctcaatg
     4681 acctcttcgc gtgtccctgg tacccagagt gccgctgcgg ccttgatacg ttttgcccct
     4741 tttgtacttt ttgccatatt ttatgatttc tccggttgtg ctgattacca caaagattcc
     4801 ggccacacga cgcgacagcc gtgcagttcg aaaacgccct gacggaaata tcccctgtgg
     4861 tcatgtccgg tatacagata acaggccttt ccctgctcaa gcatgcgcat gcaatgcgca
     4921 ctccgggaaa cgcggatgac aggtttgtta cccctgatgg tgatgctttg tacatccgtg
     4981 ttcgtcgcct taagcgccat aatggctgac tgcactttgc tgatttgctg gttgatacct
     5041 gtggtggatt tcattattaa actcctttga caacgtcagc gttgacctgt ggaaccccga
     5101 tttcagcggc cagattcatg gcggctatta ccaggttact gacggccagc ggatacagca
     5161 ggctgaccat atttttacga tgacttcccg gattgctcag gcgggcacgt atggcatcca
     5221 ctgcgctggc gtccataatg tccgtcagtt gtttaccggc ccgttgcagt ttgaacgtca
     5281 gaaattcttc aaggttattg tccagaggca gaagttcgac cacctcacag cgctgaacga
     5341 cttcacggac ttccatattg cgttcagaca gttttgtcgc cagttccggc tggccaatca
     5401 gcacgatgga cagcagtttt ttgaaaccgg actccagctc aaaaaagcgt ttgaggtgtt
     5461 tcagtgtcgg aatgggcaga ctgtgggcct cctcaatcac cagaacgtgg ctgaaccccg
     5521 cctggctgct gtctttcagg acgcgatgca actggcgaaa gcgggcgtcc tgactgcgtc
     5581 tgatgctttc cagtggtgcg atggtactga taatggcttc ggcaatagct gctgccttca
     5641 gggttttccc tttcacatcg ttgtcttcca tagcgatgat gtatggctcg ataacaatta
     5701 ccggcgcatt ctcgcggtta atacgttcag tcaggtcgcg gcgcagcgtg gatttacccg
     5761 caccggactc accgatgacg gccataaacc caccatgacg ggctgtctgg tacagcgcct
     5821 cacgcacgta gcgaatgtcc ggggtggtga acacatcatc agaaccctgc atggcttcat
     5881 cggcgaacgg gtcacggaaa agaccaaacg cttttttggt tgctggaaat aacacctgtt
     5941 ttttgagtaa catattctct tcctcactga ggctcgtttt atctgtggta cccgctgtac
     6001 ggggcgtggc cgcgccctgt acagcatcaa aactcttcgt tgtatcaatc ccctgacttt
     6061 ccagccagga cgcaagacgc cggcgcactt ctccggggct ggtgcggggc cacgcgttat
     6121 gattcacaat ctgggccagc gtggcctcag aaacatcgac agctctcgcc gccaccgcct
     6181 gtggaatacg ggcctctttc agttgctgct tcagtaccag catgtttccc tcctcagttg
     6241 ccgttaacaa tgctgataac gctgctgcgg gccggcgtgg tcagcgtggc catgacttca
     6301 tccagtgcgg cttccggtac gccgtccggg tactgtgctg ttaactggcg gtaatgttcc
     6361 ggcgtccagg tcaggccgtc ggcgctgaac ttatcgcgca gggctttcgc ggcctccaca
     6421 tgagtcatgg gacgctgttc agtgcgcggt ccgcgtacgt cagaggcctg accacgcttc
     6481 ggtatacagg ccggaagtgt ggcgtcatca atatgtttat acgggtcaag ccgcccgccg
     6541 aacggcagcg ccttcgcctt gcgtgcggca gctgcatctg cggcgttgtc tgtaccggtg
     6601 accagcgctt cggtttcttt tgccgccatc tgcgccgggg tttccggcag ggctttgtaa
     6661 ctttcgccaa ataccgccgc gccttcagca aagccaaact cgttctttct gacctcttcg
     6721 accaggaaga acgtctcgtg gccgtcctca ccggtcagaa ccacctgtgc cacatcgctg
     6781 cgccacgggt tacgggtaat catcagtttt tcaccgacca gtacgcccgg taccgttgat
     6841 acgtcaaatt cagtgccccg gaacgagaca cgaagttttg gcgtgacttt gcggagttct
     6901 ggtgccgcca cagccagttc acgacatacc tcaacggaag gcgctttttt cagctgctca
     6961 gcagtaatct tcagccagat atccgtgcgg gttttaccgt ggcggctgtg aacagccgtg
     7021 gcgttaaagt ggctgcgcca tttcgcggcc agcgcgttga gttcttccag actgtgaacc
     7081 ggccggaact tcagacccgg ctccagcttg cgttcgataa tgtcacgcgc cttttccacc
     7141 tgtccggtgg cgcgggcgtt atgcggcttg tgcgctatca ggtcgatgcc cagtgagcgg
     7201 cacatgtttt tcgtcatacc cgcggtgttt gccgagccgg ggtcgagata gagtattttt
     7261 ggcacgccgt gcagcacgtc tgcgccgcca cgctcctgca tggcgttgat aagcacagaa
     7321 cacaggttct caccggattc cgcacccgtc acatactcaa cgtaaatcca gccgctggca
     7381 tggtcggtaa tctcgtaact ccacacgcgg tcactggcga tacgggcaag gttagcgggc
     7441 ttgtttttgt agaatttcgc gctgtccatc acctgcagcc ctttatgtcc attgctcagg
     7501 tagtaaagcg tgcagagtga ggcgtcaatc tgccagacat gattggggtg cagactggcc
     7561 acttcggtat gcggtgcagg ggcatccagt tgttccgggt gcaggccata gttacgcagg
     7621 gcacgactga tggcatcttc ggacaacggg aaaaactcac cggtggtttc atctgttctg
     7681 cctgcggaga taaagccgtt agcccgcagg gtttccactg catccgcgat ggaatagaga
     7741 cgcttaccgt tcttgcgggt ggcctcacgt agtgtggcag atatcagcgc ggcttcgtcg
     7801 cgggtcaggg tgctgcgtcc ggcatcggcg cgttttttgc gtttatcagt cacagagacc
     7861 tccttcagcc tgcgcagcag agtggcgcgg gacatgccaa gttcagcaca ggcagcgtcg
     7921 tatattgcac cgcgtttacc atgccccgca ttacgtgccg cgcgggctgt ttcaacaagt
     7981 ttttcagtca gtgccggggt catagcttgt cttccctgtt atccgattct ggcggcaatt
     8041 tttctggcac ggtcgcgcag ggtttcgcca tcgcaggttt tcaccacgtt atttaccggt
     8101 tgcggcgcaa ctgccgggcg caatgctgac tgatgctgca gacgtcttat ctgctgcatc
     8161 agcatcactt ccatcgtcag tacaaacgga tgcgcctcct gtgcgggtac gcagatttca
     8221 ccggaaggtg tcaccacgcg gaaaccctgc accatctccg gcagcagatt acacagtgcg
     8281 tgccccagtg cctccgggct gtccatcagg atattttgtg tgttatccgt gttcattact
     8341 gcatgccctc cggtcggttg atttcaggct ccggttcggt cagccatgcg gggacatcgt
     8401 tacccgatgg tgcggcatca aggtcgaacg tctcacgcaa tgcatgaaca ctgcgttcaa
     8461 tctggcagac cagaccggcc atgaaatctc tgggggtatc aatcatgttt ttactgcagt
     8521 attcacacag ggtttcaaag gcaccggaga gccggacggc gatggcagat tccgcctcaa
     8581 ccgctaatgc ggtcacttcc gcccgcagct tcttcacctc ttcatcaggc tcaggcggtt
     8641 gaatacggga tttcttctcc agttttgtgg ccagttcgtt gattttttcg tcctttccgg
     8701 caagcacctg ttgctgagct gcgttagttt cgcgcgcttc acgcagggcc tggcgaagtt
     8761 cacgtactga catgcgatca acatcatcaa gcgtcaggcc ggcaactgtg ccgccgtcgg
     8821 ccag
//
LOCUS       DAMZFT010000189         8781 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.189, whole genome shotgun sequence.
ACCESSION   DAMZFT010000189 DAMZFT010000000
VERSION     DAMZFT010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8781)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8781)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8781
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.189"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(645..836)
                     /locus_tag="PN977_004643"
     CDS             complement(645..836)
                     /locus_tag="PN977_004643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="HDI6044582.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDH
                     ADELYRSCLARFGDDGENL"
     gene            900..1223
                     /locus_tag="PN977_004644"
     CDS             900..1223
                     /locus_tag="PN977_004644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HDI6044583.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSGKLPT
                     LAPPLLRQLAAIGNNLNQTARKVNSGQWSSGDRVQVVAALMAIERELRSLRQVVREQG
                     ARDDS"
     gene            1213..1923
                     /locus_tag="PN977_004645"
                     /pseudo
     CDS             1213..1923
                     /locus_tag="PN977_004645"
                     /inference="COORDINATES: protein motif:HMM:NF015397.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            1901..2419
                     /locus_tag="PN977_004646"
     CDS             1901..2419
                     /locus_tag="PN977_004646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001447511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="HDI6044584.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTEKVLNNEFRRLGESVSEAVTSN
                     ETKIRDAIALFTASTEESLEKHREGVKEAMRQHRKDVLKLAGNTGMMLLGIVFLLFTA
                     SGGTLWYLGGRIQANLEDIRKQEETLQKLNAKTWGVEFVQDGNRKFLVLPYGKSAEVI
                     PFQGKEWVHLKE"
     gene            2426..2659
                     /locus_tag="PN977_004647"
     CDS             2426..2659
                     /locus_tag="PN977_004647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="HDI6044585.1"
                     /translation="MTELETHLLNALEQLQQDYMQRLNEWESAFVELQKMFSLTQRDN
                     AMLNERVMQLSQQVQHLSEQTEHLSQLYSESRR"
     gene            complement(4539..4715)
                     /locus_tag="PN977_004648"
                     /pseudo
     CDS             complement(4539..4715)
                     /locus_tag="PN977_004648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611469.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(5241..5660)
                     /locus_tag="PN977_004649"
     CDS             complement(5241..5660)
                     /locus_tag="PN977_004649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="HDI6044586.1"
                     /translation="MRRSGGTLYLIALLTAATVLTACTPKGSVEQHTRHYVYASDDGF
                     DPNFSTQKADTTRMMVPFFRQFWDMGAKDKATGKSRSDVQQRIQQFHSQEFLNSLRGT
                     TQFAGTDYRSKDLTPKKSRLLAETISAVYLDGYEGRQ"
     gene            complement(5744..6163)
                     /locus_tag="PN977_004650"
     CDS             complement(5744..6163)
                     /locus_tag="PN977_004650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001749639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="entry exclusion protein 1"
                     /protein_id="HDI6044587.1"
                     /translation="MAWHTLQNALKMTGRSRSQFYRDMRAGLVSYRTGQDGRREFETS
                     ELIRAYGELKQDETPERHSAGQAENPHDQQTERILRELNELRQCLTLMLEDKQAQATE
                     RRRQEAEREQLHKEIEQLRQALEQEKKRGFWARLFSR"
     gene            6441..8006
                     /locus_tag="PN977_004651"
     CDS             6441..8006
                     /locus_tag="PN977_004651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-10"
                     /protein_id="HDI6044588.1"
                     /translation="METAVAYYKDGVPYDDKGQVIITLLNGNPDGSGSGGGGGTGGSK
                     SESSAAIHATAKWSTAQLKKTQAEQAARAKAAAEAQAKAKANRDALTQHLKDIVNEAL
                     RHNSTHPEVIDLAHANNAAMQAEAERLRLAKAEEKARKEAEAAEKAFQEAEQRRKEIE
                     KEQAETERQLKLAEAEEKRLAALNEEARAVEVAQKNLAAAQSELAKVDEEIKTLNTRL
                     SSSIHARDAEMNTLSGKRNELAQASAKYKELDELVKKLSPRANDPLQSRPFFYATSRR
                     TGAGKIMEEKQKQVTASETRINQLNAEINGIQGAMSQANNNRNTAVLRVHESEENLKT
                     AQTNLLNSQIKDAVDATVSFYQTLSEKYGEKYSKMAQELADKSKGKKISNVNEALAAF
                     EKYKDVLNKKFSKADRDAIFNALESVKYEDWAKHLDQFAKYLKITGHVSFGYDVVSDI
                     LKIKDTGDWKPLFLTLEKKTVDAGVSYIVVLLFSVLAGTTLGIWGIAIVTGILCAFID
                     KNKLNTINDVLGI"
     gene            complement(8003..8344)
                     /locus_tag="PN977_004652"
     CDS             complement(8003..8344)
                     /locus_tag="PN977_004652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000058758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin transporter"
                     /protein_id="HDI6044589.1"
                     /translation="MSLRYYIKNILFGLYCTLIYIYLITKNNEGYYFLASDKMLYAIV
                     ISTILCPYSKYAIEHIFFKFIKKDFFRKRKNLNNAPVAKLNLFMLYNLLCLVLAIPFG
                     LLGLFISIKNN"
     gene            8392..8529
                     /locus_tag="PN977_004653"
     CDS             8392..8529
                     /locus_tag="PN977_004653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_072023513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="HDI6044590.1"
                     /translation="MRKRFFVGILAINLLVGCQANYIRDVQGGTVAPSSSSKLTGISV
                     Q"
BASE COUNT         2355 a         1800 c         2292 g         2334 t
ORIGIN      
        1 tacaccgaac tgagatacca acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
       61 agaaaggcgg agaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
      121 cttccggggg gaaacgcctg gtatctttaa gtcctgtcgg gtttcgccac ctctgacttg
      181 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaag cgcctccggc
      241 gcggcctttt ctggggtctt cgttttttgc tcacatgttc tttccggcgt tatcccctga
      301 ttgtgtggat aacagtatca ccgcttttga gtgagcagac accgctcgcc acagccgaac
      361 gaccgagcgc agcgagtcag tgagcgagga agcggaagag catcggcatc tgcatgttct
      421 ccttacgcat ctgtgcggca tttcacaccg tacatggtgc acttttcata caatccgcac
      481 cgatgccgca tgactaagcc agtatacact ccgctatcgc tacgtgactg ggtcagggct
      541 gcgccccgac accccctaaa acctgctgac gcgccctgac gggcttgtct gctcccggca
      601 tccgctcaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg
      661 tcatccccga aacgtgcgag gcagctgcgg taaagctcat cggcgtggtc gtgaagcgat
      721 tcacagatgt ctgcctgttc atccgcgtcc agttcgttga gcttttccag cagcgttaat
      781 gtctggcttc tgataaagcg ggccatgtta agggctgttt tttcctgttt agtcactgat
      841 gcctccgtgt aagggggatt tctgttcatg gggtaatgat accgatgaaa cgcgagagga
      901 tgctcacgat acgggttact gatgatgaac atgccaggtt acttgaacgc tgtgagggta
      961 aacaactggc ggtatggatg cggcgggtat gcctgggtga gccggttgcc cgttccggga
     1021 agctgccgac actggcaccg ccgttactgc gtcagctggc tgccatcggg aataacctga
     1081 atcagacggc gaggaaggtg aacagcgggc agtggtcttc cggtgaccgg gttcaggtgg
     1141 tggccgcact gatggccatc gagagagagt tgcgcagctt acggcaggtc gtcagggaac
     1201 agggagcgcg ggatgatagt taaatttcat gccaggggaa aaggcggtgg cagtggtccg
     1261 gttgattacc tgctgggaag ggagcgtaac cgggaagggg caacggtgct tcagggtaat
     1321 ccggaagaag tccgggaact catcgatgcc acgccatttg cgaagaaata cacgtcaggt
     1381 gttctgtcgt tcgcggagaa ggagctgccg ccgggaggac gtgaaaaagt gatggcgagc
     1441 tttgagcgtg tactgatgcc cggtctcgaa aaaaatcagt acagcatcct gtgggttgag
     1501 caccaggaca agggacggct tgagctgaat tttgtcattc cgaacatgga gctacagagc
     1561 ggaaaacgcc tccagccgta ctatgaccgc gcagacaggc cgagaattga tgcctggcag
     1621 acgctggtga atcaccatta cgggctgcat gacccgaatg ccccggagaa ccgcaggacg
     1681 ctgacactcc ctgataacct gccggaaacg aaacaggcga ttgctgagag cgttacacgg
     1741 gggcttgatg tgctttaccg tgccggagag ataaaagacc gtcaggatgt gattcaggca
     1801 ctcacagatg tgggagttga ggttgtcagg gtgacgcgaa gcagtatcag cattgcagac
     1861 ccgaacggcg ggaagaatat caggctgaaa ggagcgtttt atgagcaatc ttttacagac
     1921 gggcgcggag ttcgagaaaa agctgaaaga gagagccgaa tctacagaga aagtgctgaa
     1981 caacgagttc aggaggctcg gcgaatctgt aagcgaggct gtgacatcaa acgagacgaa
     2041 aatcagagac gctatagccc tgttcacagc ctcgacagag gaatcgctgg agaaacaccg
     2101 ggaaggggtg aaagaggcga tgaggcagca caggaaggac gtgttaaagc tggcagggaa
     2161 tacgggcatg atgttactgg ggatagtctt tctcctgttt accgcgagtg gcgggacgct
     2221 ctggtatctt ggcgggagga tacaggcgaa cctggaagat atcaggaagc aggaagagac
     2281 attgcagaaa ctgaacgcga agacatgggg cgtggagttc gtgcaggacg ggaacaggaa
     2341 atttctggtc cttccgtacg ggaaatcagc agaggtgatt ccctttcagg ggaaagagtg
     2401 ggtacatctg aaggagtgac acagagtgac agagctggaa acacatttgc tgaacgcctt
     2461 agagcagctg caacaggact atatgcagcg gctgaacgaa tgggagagcg ccttcgtgga
     2521 attgcagaag atgttttcgc ttacgcaacg ggacaacgcg atgctgaacg agcgggtcat
     2581 gcagttgagt cagcaggtgc agcacttgag cgagcagaca gaacacttga gccagttata
     2641 cagcgagagc aggagataag ggacgaacta ctgatacagg aacgtgaaca tgagctattc
     2701 ctagagcgtg agcgccatct ggaaatacag gaacggacac tggatggccc gtcgctggga
     2761 tggtaaccaa agacaggcta aagtgttgtc agcgcgtttc cggtgcattt ttttggataa
     2821 tggttggttc agggcactag gaactagctt cagtcaggcc ttgctgaaaa cacaggaacg
     2881 cgttaaaacg catacggacg cgtttctggc gctcaaaaac catggggtta gtgtgggtag
     2941 tgtgtatagc aacaggagcc tggaacgcgt tccgcttctt ctgtaatcga cgccagaatg
     3001 cgtttaatgc ttcttctgat ggtatcagag aggagctgct tgagttattc ctgttggcca
     3061 cagagcgtaa ttatctcctt ttcagatcca cacggggata cgtcagaatc ggaactgagc
     3121 aaggattact gaaagatact gtttgccaga aaaaaaatcc tcatacatga tgaggataaa
     3181 gtcttaaagg tccattctat ggagtgtcgg attgaaaaat ttctccggat gatatccttc
     3241 acagtttaaa tgtgatttca accataagtt ttttatggat tttgttaaat aaaaaagaat
     3301 tgagtcgtgt ttctgatatg tctgaatata ataagtgaac agttataaaa taattgtttt
     3361 atatttattg tgagtttgat gttatgttct gcataaataa ctgtagttta gaatattatt
     3421 cttttatatt tatagctgtg agatgataaa ttataagtgt tctttgtgct ataaagaacc
     3481 ccccgtataa gggggggtaa aagatgggtt tacttctttt tagtccatgc cctggtgagc
     3541 tacatgaagc tcgacactac agtaaaaatt tgcagttttt ttgaacttta cgcaagcaat
     3601 ttatttacat tgtaaagaaa accccctcaa ttccggaggg ggagtgtagg gaagtgcctg
     3661 atttatttta tgtacgcgca aaaatgagat agcttttatt agatatcatc agccctgtct
     3721 gcatcagtca gctccttaca gcgtcaggag tactgatgaa ggggggaacg ttcctgcgtt
     3781 tttgcaaagg caatccggct agaggcttta ctacgcagaa acattccgga caacttttta
     3841 ccccagtgta acttgtagat caaccagatt ttgttgcagt aaaaatacag tggtttcgaa
     3901 tttgtaacat ttgtcacaaa aataacatca atatgttggt tttctgttga taaatggttt
     3961 ggtcgctgat tatgatttcc ttgtactcca caggagtaca gtcatgtgca tactaaaatc
     4021 cgtgttttcg ataaaattaa ggccgtgata cagaatcacg gcttttttct tgcctgaaag
     4081 gatcatttac gctagaagaa ttatgagcgc gtctctggtg tttgttgcag ggataatatc
     4141 taatatgggg caccggaaac gcgctctccc ttcgtataca gaatgcccat taatttcttc
     4201 ttcctgattt tccagacata tattcttgtg taaattaaaa aacagagcgt gtttctggtg
     4261 tgaatttgtt ggctacaatg tgcattgttg ttctgtgttc cagaaacacg cttctttttt
     4321 gagatgcaca tcggtcttat acaaagaggg atggttgtat ctgtgtcaga gttttgttat
     4381 tttttgtatg ctcagttatt gatggttaat aatatcaaga aaatcagcaa gttccttaca
     4441 ctacccctct tcagtagagg ggttttttta tctggtacgc cgtaaaaccc ggtccttcag
     4501 ggcgtagagg atgtcaaaaa tttcttcgaa aggtaatacc cggtatcccg ctcatgctgg
     4561 cgaacccaga cacgcagggt ctctggcgta cagccaatct ttggagcaat ggaacaaatt
     4621 gccgcccatt gtgagtcata ttcgccctgg ctttccagaa ccatacgaac tgcccgttga
     4681 cggacttcgg gggaaaaacg agtattttta gtcatcctgt ttacatcttt ctcaggaagt
     4741 ttagtctcca ggatttccgg ggcggttcag tccggcagag ataaattgcc acgggaagtt
     4801 agagaaaagt gcgggaaaat gtcttaagaa tgattctgga agaacgaaaa gtaagaagcg
     4861 caaaggctat gagggcaaaa gagtatcgtg ccgcagcgaa tacttttacc tggcagcctg
     4921 tacagcgcag gtgaatagac attcccctga ctcatcctgc tccccttggc ggcgaagggg
     4981 attaatccct atcctgtaac ttctattttg tggaatcgaa aacagctgaa aattgtgaaa
     5041 aaaaaccgtt tcagtcgtct cagtaaggaa attcgcttgt gattcacctg gatgcatagg
     5101 tatgcataag gttaaaattt atcaggcgcg atcgcggtag tttttcgggg gggttgttgc
     5161 gatttttacc tgtctgctgc cgtgattgcg ctgaatgcat ttcagcggtg cgtacaatta
     5221 agggattatg gtaaatccac ttactgtctt ccctcgtagc catcgagata aaccgcagaa
     5281 atcgtctcag ccagcagcct ggattttttc ggggtaaggt ctttgctgcg gtaatcagta
     5341 cccgcaaatt gagttgtgcc ccggagtgag tttaaaaatt cctggctgtg aaactgctga
     5401 atgcgttgct gcacatcact ccgtgatttt cctgtcgctt tatcctttgc tcccatatcc
     5461 cagaactgcc ggaaaaaagg caccatcatt cgtgttgtgt cggccttctg ggtggaaaag
     5521 ttaggatcaa aaccatcgtc tgatgcataa acgtaatgcc gggtatgttg ttccacactg
     5581 ccctttggcg tgcaggctgt caggaccgtt gccgctgtca gcagggcgat aagatacagt
     5641 gtgccgccgg aacgtctcat gagtgtctcc ttacctttaa atctttgtac tcagtgactt
     5701 atatttaacc gctgactatc ttatcatcac ttgcagtcgg tgctcaccgg ctgaacagtc
     5761 tggcccagaa tccccgtttt ttttcctgct ccagtgcctg cctgagctgc tctatctctt
     5821 tatgtagctg ttcccgttcg gcttcctggc gccggcgttc cgtagcctgt gcctgtttgt
     5881 cctcaagcat cagtgtcagg cattgtctca gctcattcag ctcccggaga atgcgttctg
     5941 tctgctgatc atgtggattt tctgcctgtc ccgcactgtg cctttctggt gtctcatcct
     6001 gctttaattc gccgtatgcc cggatcagtt cactggtttc aaactcccgc cgtccgtcct
     6061 ggcctgtacg ataactgaca aggcctgcgc gcatatcacg gtaaaactgt gaacgcgatc
     6121 tgcctgtcat tttcagtgcg ttctggagtg tgtgccatgc catgaagtga cagtgtccca
     6181 ttaatgtctc atatcatggt ttcagtaaaa catactgggg ccggagaacg gtaatgtttg
     6241 tgctggtttt tgtggcatcg ggtgggaata gcacgtggtg tgggtggctg tttttttgat
     6301 cgttttcaca aaaatgaaaa tccacaaggt tgacagggaa aatgcagcgg cgtaatttta
     6361 agctgtatat aaaaccagtg gttatatata cagtatattg cttttaacta tttgttttta
     6421 atgtcaaaga ggattttata atggaaacag ctgtagcgta ctataaagat ggtgttcctt
     6481 atgatgataa ggggcaggta atcattactc ttttgaatgg taatccagac gggagtggct
     6541 ctggcggcgg tggtggaact ggaggtagca aaagtgaaag ttctgcagcc attcatgcca
     6601 cagctaaatg gtctactgct caattgaaga aaacgcaggc agaacaggct gcccgggcaa
     6661 aagctgccgc agaagcacag gctaaagcaa aagcaaaccg ggatgcgctg actcaacatc
     6721 tgaaggatat tgtgaatgag gcgcttcgtc ataattccac tcatccggag gttattgacc
     6781 tggctcatgc caataatgca gcgatgcagg cagaagcaga gcggttgcgc cttgcaaaag
     6841 cagaagaaaa agcccgtaaa gaagcggaag ctgcggaaaa ggcttttcag gaggcagaac
     6901 aacgacgtaa agagatagag aaggagcagg ctgaaacaga acgccagttg aaactggctg
     6961 aagctgaaga gaaacggctg gcagcattga atgaagaggc ccgggcagtg gaggtggcac
     7021 aaaaaaatct tgctgctgca caatctgagc tggcgaaagt ggatgaagag attaagactc
     7081 tcaatacccg tttaagctcc agtattcatg cccgtgatgc agaaatgaat acgctgtccg
     7141 gaaaacgaaa tgagctggct caggcatctg ctaaatataa ggaacttgat gaactggtca
     7201 aaaaattgtc accaagagct aatgatccgc ttcagagtcg tccttttttc tatgccacca
     7261 gtcgacggac gggagccggt aagattatgg aggaaaaaca aaaacaggta acagcatcag
     7321 aaacgcgtat taaccaactt aatgctgaga taaatggaat tcagggggct atgtctcagg
     7381 ctaataataa tcgtaataca gctgttttac gtgttcatga atctgaagaa aatttgaaaa
     7441 cagcgcagac taatctcctg aactcgcaga ttaaggatgc tgtggatgca acagttagct
     7501 tttatcaaac gctatctgaa aaatatggtg aaaaatattc aaaaatggca caggaacttg
     7561 ctgataagtc taaaggtaag aaaatcagca atgtgaatga agctctagct gcttttgaaa
     7621 aatacaagga tgttttaaat aagaaattca gcaaagcaga ccgtgatgcg attttcaatg
     7681 cactggaatc ggttaagtat gaagactggg ctaagcattt agatcagttt gctaagtact
     7741 tgaagattac gggacatgtt tcttttggat atgatgtggt atctgatatc ctaaaaatta
     7801 aggatacagg tgactggaag ccactatttc ttacattaga gaagaaaact gtagatgctg
     7861 gagttagtta tattgttgtt ttacttttta gtgtgcttgc tggaactaca ttaggtatct
     7921 gggggattgc tattgttaca ggcattctat gtgcctttat tgacaagaat aaacttaata
     7981 ctataaatga tgtgttgggt atttaattat tctttattga tataaacagt cctagcaatc
     8041 caaatgggat tgctaggacc aaacaaagta gattgtatag cataaatagg tttaattttg
     8101 ccacgggggc attatttaga ttttttcttt ttctgaaaaa atctttcttt atgaacttaa
     8161 aaaatatgtg ttcaatagca tattttgaat atgggcatag aatagtgctt atcacgattg
     8221 cgtatagcat cttatctgac gctaggaaat aatacccttc gttgtttttt gttataaggt
     8281 atatatatat aagtgtgcaa tataggccaa acaaaatgtt ttttatgtag tatcttaaac
     8341 tcataaatta aacctcgtta tatattcttt tcattttata aggatcgagt tatgaggaaa
     8401 agattttttg tgggaatatt agctataaac ctccttgttg gatgtcaggc taactatata
     8461 cgtgatgttc agggagggac tgtggctcca tcatcttctt ctaaactgac ggggatctct
     8521 gttcagtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct
     8581 gcctgtaaac gaaaaaaccg cctggggagg cggtttgatc gaaggttaag tcagttgggg
     8641 aactgcttaa ccgaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcca
     8701 gtgtagccgt agtcgggcca ctacttcaag aactctgttg caccatttgc gccatcatcg
     8761 ctctgctaac caagttacca a
//
LOCUS       DAMZFT010000190         8720 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.190, whole genome shotgun sequence.
ACCESSION   DAMZFT010000190 DAMZFT010000000
VERSION     DAMZFT010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8720)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8720)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8720
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.190"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..572
                     /locus_tag="PN977_004654"
     CDS             <1..572
                     /locus_tag="PN977_004654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310359.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HDI6044591.1"
                     /translation="QVRDRWREQGGPGSGIWYDLAPHLLDQAITLFGLPVSMTVDLAQ
                     LRPGAQSTDYFHAILSYPQRRVILHGTMLAAAESARYIVHGSRGSYVKYGLDPQEERL
                     KNGERLPQEDWGYDMRDGVLTRVEGEERVEETLLTVPGHYPAYYAAIRDALNGDGENP
                     VPASQAIQVMELIEQGIESAKHRATLCLA"
     gene            complement(608..1609)
                     /gene="add"
                     /locus_tag="PN977_004655"
     CDS             complement(608..1609)
                     /gene="add"
                     /locus_tag="PN977_004655"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HDI6044592.1"
                     /translation="MIDTTLPLTDIHRHLDGNIRPQTILELGRRYNISLPAQSLETLI
                     PHVQVIANEPDLVSFLTKLDWGVKVLASLDACRRVAFENIEDAARNGLHYVELRFSPG
                     YMAMAHQLPVAGVVEAVIDGVREGCRTFGVQAKLIGIMSRTFGEAACQQELEAFLAHR
                     DQITALDLAGDELGFPGSLFLSHFNRARDADWHITVHAGEAAGPESIWQAIRELGAER
                     IGHGVKAIEDRALMDFLAEQQIGIESCLTSNIQTSTVAELAAHPLKMFLEHGIRASIN
                     TDDPGVQGVDIIHEYTVAAPAAGLSREQIRQAQINGLEMAFLSAEEKRALREKVAAK"
     gene            complement(1713..2885)
                     /gene="malY"
                     /locus_tag="PN977_004656"
     CDS             complement(1713..2885)
                     /gene="malY"
                     /locus_tag="PN977_004656"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional maltose regulon transcriptional
                     repressor/cystathionine beta-lyase MalY"
                     /protein_id="HDI6044593.1"
                     /translation="MFDFSKVVDRHGTWCTQWDYVADRFGTADLLPFTISDMDFATAP
                     CIIEALNQRLMHGVFGYSRWKNDEFLAAIAHWFSTQHYTAIDPQSVVYGPSVIYMVSE
                     LIRQWSETGEGVVIHTPAYDAFYKAIEGNQRTVIPVALEKQADGWFCDMGKLEAVLAK
                     PECKIMLLCSPQNPTGKVWTCDELEIMADLCERHGVRVISDEIHMDMVWGEQPHIPWC
                     NVARGDWALLTSGSKSFNIPALTGAYGIIENSSSRDAYLSALKGRDGLSSPSVLALTA
                     HIAAYQQGAPWLDALRVYLKDNLTYIADKMNAAFPELNWQIPQSTYLAWLDLRPLNID
                     DNALQKALIEQEKVAIMPGYTYGEEGRGFVRLNAGCPRSKLEKGVAGLINAIRAVR"
     gene            complement(2895..4487)
                     /gene="malX"
                     /locus_tag="PN977_004657"
     CDS             complement(2895..4487)
                     /gene="malX"
                     /locus_tag="PN977_004657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS maltose transporter subunit IICB"
                     /protein_id="HDI6044594.1"
                     /translation="MTAKTAPKVTLWEFFQQLGKTFMLPVALLSFCGIMLGIGSSLSS
                     HDVITLIPVLGNPVLQAIFTWMSKIGSFAFSFLPVMFCIAIPLGLARENKGVAAFAGF
                     VGYAVMNLAVNFWLTNKGILPTTDAAVLKANNIQSILGIQSIDTGILGAVIAGIIVWM
                     LHERFHNIRLPDALAFFGGTRFVPIISSLVMGLVGLVIPLVWPIFAMGISGLGHMINS
                     AGDFGPMLFGTGERLLLPFGLHHILVALIRFTDAGGTQEVCGQTVSGALTIFQAQLSC
                     PTTHGFSESATRFLSQGKMPAFLGGLPGAALAMYHCARPENRHKIKGLLISGLIACVV
                     GGTTEPLEFLFLFVAPVLYVIHALLTGLGFTVMSVLGVTIGNTDGNIIDFVVFGILHG
                     LSTKWYMVPVVAAIWFVVYYVIFRFAITRFNLKTPGRDSEVASSIEKAVAGAPGKSGY
                     NVPAILEALGGADNIVSLDNCITRLRLSVKDMSLVNVQALKDNRAIGVVQLNQHNLQV
                     VIGPQVQSVKDEMAGLMHTVQA"
     gene            4662..5690
                     /gene="malI"
                     /locus_tag="PN977_004658"
     CDS             4662..5690
                     /gene="malI"
                     /locus_tag="PN977_004658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mal regulon transcriptional regulator MalI"
                     /protein_id="HDI6044595.1"
                     /translation="MATAKKITIHDVALAAGVSVSTVSLVLSGKGRISTATGERVNAA
                     IEELGFVRNRQASALRGGQSGVIGLIVRDLSAPFYAELTAGLTEALEAQGRMVFLLHG
                     GKDGEQLAQRFSLLLNQGVDGVVIAGAAGSSDDLRRMAEEKAIPVIFASRASYLDDVD
                     TVRPDNMQAAQLLTEHLIRNGHQRIAWLGGQSSSLTRAERVGGYCATLLKFGLPFHSD
                     WVLECTSSQKQAAEAITALLRHNPTISAVVCYNETIAMGAWFGLLKAGRQSGESGVDR
                     YFEQQVSLAAFTDATPTTLDDIPVTWASTPARELGTTLADRMMQKITHEETHSRNLII
                     PARLIAAK"
     gene            5802..6569
                     /gene="hdhA"
                     /locus_tag="PN977_004659"
     CDS             5802..6569
                     /gene="hdhA"
                     /locus_tag="PN977_004659"
                     /EC_number="1.1.1.159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-alpha-hydroxysteroid dehydrogenase"
                     /protein_id="HDI6044596.1"
                     /translation="MFNSDNLRLDGKCAIITGAGAGIGKEIAITFATAGASVVVSDIN
                     ADAANHVVNEIQQLGGQAFACRCDITSEQELSALADFAVSKLGKVDILVNNAGGGGPK
                     PFDMPMADFRRAYELNVFSFFHLSQLVAPEMEKNGGGVILTITSMAAENKNINMTSYA
                     SSKAAASHLVRNMAFDLGEKNIRVNGIAPGAILTDALKSVITPEIEQKMLQHTPIRRL
                     GQPQDIANAALFLCSPAASWVSGQILTVSGGGVQELN"
     gene            6800..7387
                     /gene="uidR"
                     /locus_tag="PN977_004660"
     CDS             6800..7387
                     /gene="uidR"
                     /locus_tag="PN977_004660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000369692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator UidR"
                     /protein_id="HDI6044597.1"
                     /translation="MDNMQTEAQPTRTRILNAAREIFSENGFHSASMKAICKSCAISP
                     GTLYHHFISKEALIQAIILQDQERALARFREPIEGIHFVDYMVESIVSLTHEAFGQRA
                     LVVEIMAEGMRNPQVAAMLKNKHMTITEFVAQRMRDAQQKGEISPDINTAMTSRLLLD
                     LTYGVLADIEAEDLAREASFAQGLRAMIGGILTAS"
     gene            7777..>8720
                     /locus_tag="PN977_004661"
     CDS             7777..>8720
                     /locus_tag="PN977_004661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HDI6044598.1"
                     /translation="MLRPVETPTREIKKLDGLWAFSLDRENCGIDQRWWESALQESRA
                     IAVPGSFNDQFADADIRNYVGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKVWVNNQ
                     EVMEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSY
                     FHDFFNYAGIHRSVMLYITPNTWVDDITVVTHVAQDCNHASVDWQVVANGDVSVELRD
                     ADQQVVATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYPLRVGIRSVAV
                     KGEQFLINHKPFYFTGFGRHEDADLRGKGFDNVLMVHD"
BASE COUNT         2226 a         2146 c         2273 g         2075 t
ORIGIN      
        1 cgcaggtgcg cgatcgttgg cgtgaacagg gcggtcctgg cagcggtatc tggtacgatt
       61 tagcaccgca tcttcttgat caggccatta cgctatttgg tttaccggtc agcatgacgg
      121 ttgatttggc acagttacgg cccggagcgc agtcgaccga ttatttccac gccatcttgt
      181 cctatccgca gcggcgagtc attttacacg gtaccatgct ggcagctgct gagtcagcac
      241 gttatatcgt gcatggatcc cgaggcagtt atgtgaaata tggcctcgat ccacaggaag
      301 aacgtctgaa aaatggcgag cgtctgccgc aggaagactg gggctacgat atgcgtgatg
      361 gcgtacttac ccgcgtggaa ggtgaggaac gtgtcgaaga aacgctgttg acagtaccag
      421 ggcattatcc ggcttactat gcggctattc gtgatgcgtt aaatggcgat ggtgaaaatc
      481 cggttccggc aagtcaggca atccaggtaa tggagttgat tgagcagggc atcgaatccg
      541 ccaaacatcg cgcgacgctg tgccttgcgt gaaaagagga tggtgcaatt agcaccatcc
      601 atttttgtta cttcgcagcg actttttctc gcagtgcgcg tttttcctct gcgctgagga
      661 aagccatttc cagaccatta atctgcgcct ggcggatttg ctcgcgggat aacccagcag
      721 ccggcgcggc aacggtatat tcgtgaatga tatccactcc ctgtacgccg ggatcgtcag
      781 tgttaatgct ggcacgaatg ccatgctcaa ggaacatttt cagcggatgt gccgccagct
      841 ctgccacggt gctggtctga atattggagg tcagacagga ttcaatacca atttgctgct
      901 cggcgagaaa atccatcagc gcccgatctt caatggcttt tacgccatgt ccaatacgtt
      961 ccgcccccag ttcacgaatc gcctgccaga tgctttccgg cccggcagct tcgcctgcat
     1021 ggacggtaat atgccagtcc gcatcacgcg cgcggttgaa gtgagaaagg aacagacttc
     1081 ccgggaaacc aagttcatca ccggctaaat caagtgcggt aatctggtca cggtgggcta
     1141 aaaaggcctc cagctcttgc tgacaggcgg cttcaccgaa ggtccggctc ataatgccga
     1201 taagcttcgc ctgcacacca aaggtgcggc aaccttcacg tacgccatcg atcaccgctt
     1261 cgacaacacc cgctacaggc agctgatgtg ccattgccat gtagcccggt gaaaaacgca
     1321 gctcgacata gtgcaggccg ttacgggctg catcttcaat gttttcaaat gccacgcggc
     1381 gacaggcatc aagagaggcg agaactttaa cgccccagtc aagtttggtc agaaagctca
     1441 caagatcggg ttcgttggca atgacctgaa cgtggggaat cagtgtttcc agggattgtg
     1501 caggaagcga gatattatac cggcggccaa gttcaagaat ggtctgggga cgaatgttgc
     1561 catcaaggtg gcgatggata tcagttaatg gcagggtggt atcaatcatg gtcgcactct
     1621 ttttctggat ataaaagtgc gcaccattat aaaaacaaag cagggtaaaa atccacgatt
     1681 gcgcaacgat tttttacgtt gcgcaattgg ggttaacgaa cagcgcggat ggcgttaatt
     1741 aatccagcaa cacctttttc cagtttcgaa cgcgggcacc cggcattgag tcggacaaaa
     1801 ccacgacctt cttcaccgta ggtatatccc ggcatgatcg cgaccttttc ttgctcgata
     1861 agcgcttttt gcaacgcgtt gtcgtcaatg ttcaatggac gtagatcaag ccaggccaga
     1921 taagtggatt gcgggatctg ccagttgagt tcaggaaacg cagcgttcat tttatctgcg
     1981 atatacgtca ggttatcttt cagatagacg cgtaaggcat ccagccacgg cgcgccttgc
     2041 tgataggcgg cgatatgagc agttaacgcc agtaccgaag gggaagaaag cccatcacgt
     2101 cctttcagtg ccgataaata ggcatcgcgg ctactgctat tttctataat cccgtaagca
     2161 ccggtcaggg cgggaatatt gaaacttttt gatcccgacg ttagcaacgc ccagtctccg
     2221 cgcgccacat tacaccatgg aatatgcggc tgctcgcccc aaaccatatc catatggatt
     2281 tcatcggaaa taacccgcac accatgacgc tcgcacaggt cagccatgat ctccagctca
     2341 tcgcacgtcc acactttccc ggtaggattc tgtgggctac acaggagcat aattttacat
     2401 tctggtttcg ccagcacggc ttccagcttg cccatatcgc aaaaccagcc gtcagcctgc
     2461 ttctctaaag caacgggtat tactgtgcgc tggttacctt caatggcctt gtaaaatgcg
     2521 tcataggcgg gtgtgtggat caccacgcct tcacctgttt cagaccactg acgaatcagt
     2581 tctgaaacca tatagatgac agaagggcca tacaccaccg actggggatc gatggcggtg
     2641 taatgctggg tggaaaacca gtgggcaata gccgcgagaa actcatcgtt tttccagcgg
     2701 ctgtagccaa acacgccgtg catcagacgc tgattcagcg cctcgataat gcagggggca
     2761 gtggcaaaat ccatgtctga aatcgtgaac ggtaacaggt cagcagtgcc gaaacggtcg
     2821 gcgacataat cccactgcgt acaccatgtg ccatgacgat ccacgacctt tgaaaaatcg
     2881 aacatatctt atccttatgc ctggacagta tgcatcagac cggccatttc atcttttact
     2941 gactgaactt gtggcccgat aacaacctgc aggttatgtt gattaagttg tactacgcca
     3001 attgcccgat tgtccttcag tgcctgcaca ttaacaagcg acatatcttt tacagacaaa
     3061 cgcagacggg taatgcagtt atcgagactg acaatattgt cggctccgcc taaggcttcg
     3121 aggattgccg gaacgttgta acctgattta cccggcgcac cggcaacggc tttttcgatt
     3181 gaactggcaa cttcactatc gcgccccggg gttttcagat tgaagcgggt gatagcgaaa
     3241 cggaagatga cgtagtaaac gacaaaccat attgccgcca ctactggcac catgtaccac
     3301 ttggttgaca gaccatgcaa aataccgaac accacaaagt cgatgatatt gccgtcggta
     3361 ttaccgatgg tgacgccgag cacagacatg acagtgaagc cgaggccggt taacagcgcg
     3421 tggatgacat acagaactgg cgctacgaac aggaacagga attccagcgg ttcggtcgtg
     3481 ccgccaacga cacaggcgat caggccagaa atcagcagac ctttaatttt atggcgattt
     3541 tccgggcgcg cacagtgata catagctaac gctgcacctg gcagaccgcc gagaaacgca
     3601 ggcattttac cttgcgaaag gaaacgcgtg gcgctttcag aaaaaccgtg agtggtcggg
     3661 caactcaatt gcgcctggaa gatggtcagt gcaccgctga cggtttgacc gcagacttcc
     3721 tgcgtgccgc ctgcgtcggt aaagcgaatt aatgccacca gaatgtgatg cagaccaaac
     3781 ggcaacagca gacgttcacc ggtaccaaac agcatcggtc cgaaatcacc cgcgctgttg
     3841 atcatatggc ctaagccgct aatacccatg gcgaaaatcg gccagactaa tggaatcacc
     3901 aggccgacaa gacccatcac cagcgaggag atgattggta cgaagcgcgt accgccgaag
     3961 aatgccagcg catccggcag gcggatatta tggaaacgct catgcagcat ccagacgata
     4021 ataccggcga tcaccgcacc gaggatcccg gtgtcgatcg actggatccc aagaatgctc
     4081 tggatgttat tggctttcag aaccgcggcg tccgtggttg gcagaatgcc tttattggtc
     4141 aaccagaagt ttaccgcgag gttcattacc gcataaccga cgaagccagc gaatgccgct
     4201 acgcctttgt tttcacgtgc cagacccagc gggatggcga tacagaacat gactggcagg
     4261 aaactaaaag caaacgagcc aatcttactc atccaggtaa agatagcctg caacacgggg
     4321 ttgcccagga ccgggatcag ggtgatgaca tcatggctgc taagagaact accaatgccg
     4381 agcataatgc cgcagaacga caataatgcc acgggtaaca taaaggtttt gcctaactgc
     4441 tggaagaact cccacagcgt gactttcggt gctgttttcg ccgtcataaa acgactcctc
     4501 gtagagaaca atctggattc agtaaattgc gcgagaagat aaaacgtttt atcaaatttt
     4561 agtgaggcat aaatcacatt acgcaacgat aatagcgggt ataagataaa taaaaggtaa
     4621 aacgttttat ctgtcacata atcagggagt aggtcatctg catggctacc gccaaaaaaa
     4681 taaccattca tgatgttgcg ctggctgcgg gcgtgtcggt aagtaccgtt tcgctggtgc
     4741 taagtggcaa agggcgaatc tctaccgcca caggagaacg cgttaacgcc gccattgaag
     4801 agctgggatt tgtgcgcaat cgtcaggcgt cggcgctgcg cggcgggcaa agcggcgtca
     4861 ttggtttgat cgtccgtgat ttatctgcgc cgttttacgc cgaattaacg gccggattga
     4921 cggaagctct ggaagcgcag ggacggatgg tttttttgct tcacggcggt aaagacggcg
     4981 agcagctggc acaacggttt tcactgttac tgaatcaggg ggtcgatggt gtggtcattg
     5041 ccggggctgc aggaagcagc gatgacctgc gacggatggc agaagaaaaa gctatcccgg
     5101 ttattttcgc ttcccgtgcc agttatcttg atgatgttga tacggttcgc ccggacaaca
     5161 tgcaggctgc acagttgttg acggagcatc tcattcgcaa tgggcatcag cgtatcgcct
     5221 ggctgggagg gcaaagttcc tcattaaccc gggcagaacg ggtggggggc tattgtgcga
     5281 ctctactaaa atttggcctg ccgtttcaca gcgattgggt gctggagtgc acttccagcc
     5341 agaagcaagc cgcggaagct atcacggcgc ttttacgtca taacccgacc atcagcgccg
     5401 tggtttgcta taacgaaact attgcgatgg gtgcatggtt tggtttgctg aaagcaggca
     5461 ggcaaagcgg ggaaagcgga gtcgatcgtt actttgagca acaggtttcg ctggcggcat
     5521 ttaccgatgc aacgcctacc acacttgatg atatacccgt tacctgggcc agcacgccag
     5581 cgcgggaact tggtaccaca cttgcggatc gcatgatgca aaaaatcacc catgaagaga
     5641 cgcattcacg caatcttatt attcccgccc ggctcattgc ggcgaaataa tcctctcttt
     5701 atctgctata cctggtagag tcctttcctc aaggttaatg ataaaaaatg tggcacacag
     5761 gcggtgcctt ttatttttac tgaagttcac aggaggttta tgtgtttaat tctgacaacc
     5821 tgagactcga cggaaaatgc gccatcatca caggtgcggg tgcaggtatt ggtaaagaaa
     5881 ttgccattac attcgcgaca gctggcgcat ctgtggtggt cagtgatatt aatgccgatg
     5941 cagctaatca tgttgtaaat gaaattcaac aactgggtgg tcaggcattt gcctgccgct
     6001 gcgatattac ttccgaacag gaactctctg cactggcaga ctttgccgtc agtaagttgg
     6061 gtaaagttga tatcctggtt aacaacgccg gtggcggtgg tcctaaaccg tttgatatgc
     6121 caatggcaga ttttcgccgc gcttatgaac tgaatgtatt ttcttttttc catctgtcac
     6181 aacttgttgc gccagaaatg gaaaaaaatg gcggtggcgt tattttgacc attacttcta
     6241 tggcggcaga aaataaaaat ataaacatga cctcctatgc atcatctaaa gctgcggcca
     6301 gtcatctggt cagaaatatg gcgtttgacc ttggtgaaaa aaatattcgg gtgaatggca
     6361 ttgcgccggg ggcaatatta accgatgccc tgaaatccgt tattacacca gaaattgaac
     6421 agaaaatgtt gcaacacaca ccaatcagac gtctgggcca accgcaagat atagctaacg
     6481 cggcgctgtt cctttgctcg cctgcagcca gctgggtaag cggacaaatt ctcaccgtct
     6541 ccggtggtgg ggtacaggag ctcaattaat acactaacga accggtaaac agccgtatgt
     6601 gttgtttacc gggatagcgt gtgcaactca tcaacgtctc tgctaaacaa ctggcagcca
     6661 aatcacggct attggttaac caatatctgg ataaaaagct tacgaattga gtgtgccttc
     6721 gcactattca acagcaatga taggcgctca ccggacaacg cggtaaacta gttattcatg
     6781 ctaactatat tggttaacga tggataacat gcaaactgaa gcacaaccga cacggacccg
     6841 gatcctcaat gctgccagag agattttttc agaaaatgga tttcacagtg cctcgatgaa
     6901 agccatctgt aaatcttgcg ccattagtcc cgggacgctc tatcaccatt tcatctccaa
     6961 agaagccctg attcaggcga ttatcttaca ggaccaggag agggcgctgg cccgtttccg
     7021 ggaaccgatt gaagggattc atttcgttga ctatatggtc gagtctattg tctctctcac
     7081 ccatgaagcc tttgggcaac gggcgctggt ggtcgaaatt atggcggaag ggatgcgtaa
     7141 cccacaggtc gccgccatgc ttaaaaataa gcatatgacg atcacggaat ttgttgccca
     7201 gcggatgcgt gatgcccagc aaaaaggcga gataagccca gatatcaaca cggcaatgac
     7261 ttcacgttta ctgctggatc tgacctacgg tgtactggcc gatatcgaag cggaagatct
     7321 ggcgcgtgaa gcgtcgtttg ctcagggatt acgcgcgatg attggcggta tattaaccgc
     7381 atcctgatcc tctctctctt tccgcgggct ggtgacaact gagcccgcgt ttcatatcgt
     7441 aatttctctg tgcaaaaatc atcctccccg ggatcggaga atcccaccta aatattcatc
     7501 gtagcaatat atcatgaggg tttatcgttc ccaatatgtt cgaacgaacg ttcgattgct
     7561 tattttgtgg cttctgtcaa cgctgtttta aagattaatg cgatcaatat cacgctgtgg
     7621 gtattgcagt ttttggtttt ttgatcgcgg tgtcagttct ttttatttcc atttctcttc
     7681 cgtgggtttc tcacagataa ctgtgtgcaa cacagaattg gttaactaat cagattaaag
     7741 gttgaccagt atttttatct taatgaggag tcccttatgt tacgtcctgt agaaacccca
     7801 acccgtgaaa tcaaaaaact cgacggcctg tgggcattca gtctggatcg cgaaaactgt
     7861 ggaattgatc agcgttggtg ggaaagcgcg ttacaagaaa gccgggcaat tgctgtgccg
     7921 ggcagtttta acgatcagtt cgccgatgca gatattcgta attatgtggg caacgtctgg
     7981 tatcagcgcg aagtctttat accgaaaggt tgggcaggcc agcgtatcgt gctgcgtttc
     8041 gatgcggtca ctcattacgg caaagtgtgg gtaaataatc aggaagtgat ggagcatcag
     8101 ggcggctata cgccatttga agccgatgtc acgccgtatg ttattgccgg gaaaagtgtt
     8161 cgtatcaccg tttgtgtgaa caatgaactg aactggcaga ctatcccgcc gggaatggtg
     8221 attaccgatg aaaacggcaa aaaaaagcag tcttacttcc atgatttctt taactatgcc
     8281 gggatccatc gcagcgtaat gctctacatc acgccgaaca cctgggtgga cgatatcacc
     8341 gtggtgacgc atgtcgcgca agactgtaac cacgcgtctg ttgactggca ggtggtagca
     8401 aatggtgatg tcagcgttga actgcgtgat gcggatcaac aggtggttgc aactggacaa
     8461 ggcaccagcg ggactttgca agtggtgaat ccgcacctct ggcaaccagg tgaaggttat
     8521 ctctatgaac tgtgcgtcac agctaaaagc cagacagagt gtgatatcta cccgctgcgc
     8581 gtcggtatcc ggtcagtggc agtgaagggc gaacagttcc tgatcaacca caaaccgttc
     8641 tactttactg gctttggtcg tcatgaagat gcggatttgc gcggcaaagg attcgataac
     8701 gtgctgatgg tgcacgatca
//
LOCUS       DAMZFT010000191         8523 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.191, whole genome shotgun sequence.
ACCESSION   DAMZFT010000191 DAMZFT010000000
VERSION     DAMZFT010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8523)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8523)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8523
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.191"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..782
                     /gene="amiA"
                     /locus_tag="PN977_004662"
     CDS             <1..782
                     /gene="amiA"
                     /locus_tag="PN977_004662"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiA"
                     /protein_id="HDI6044599.1"
                     /translation="QAIAKEEPLKTSNGHSKPKAKKNGGKRVVVLDPGHGGIDTGAIG
                     RNGSKEKHVVLAIAKNVRSILRNHGIDARLTRSGDTFIPLYDRVEIAHKHGADLFMSI
                     HADGFTNPKAAGASVFALSNRGASSAMAKYLSERENRADEVAGKKATDKDHLLQQVLF
                     DLVQTDTIKNSLTLGSHILKKIKPVHKLHSRNTEQAAFVVLKSPSVPSVLVETSFITN
                     PEEERLLGTAAFRQKIATAIAEGVISYFHWFDNQKAHSRKR"
     gene            786..1685
                     /gene="hemF"
                     /locus_tag="PN977_004663"
     CDS             786..1685
                     /gene="hemF"
                     /locus_tag="PN977_004663"
                     /EC_number="1.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-dependent coproporphyrinogen oxidase"
                     /protein_id="HDI6044600.1"
                     /translation="MKPDAHQVKQFLLNLQDTICQQLSAVDGAEFVEDSWLREAGGGG
                     RSRVLRNGGVFEQAGVNFSHVHGEVMPASATAHRPELAGRSFEAMGVSLVVHPHNPYV
                     PTSHANVRFFIAEKPGAEPVWWFGGGFDLTPFYGFEEDAIHWHRTARDLCQPFGEDVY
                     PRYKKWCDEYFYLKHRNEQRGIGGLFFDDLNTPDFDHCFAFMQAVGKGYTNAYLPIVE
                     RRKTMAYGERERNFQLYRRGRYVEFNLVWDRGTLFGLQTGGRTESILMSMPPLVRWEY
                     DYQPKDGSPEAALNEFIKVRDWV"
     gene            complement(1691..2743)
                     /gene="eutR"
                     /locus_tag="PN977_004664"
     CDS             complement(1691..2743)
                     /gene="eutR"
                     /locus_tag="PN977_004664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator EutR"
                     /protein_id="HDI6044601.1"
                     /translation="MKKTRTANLHHLYHEPLPENLKLTPKVEVDNVHQRQTTDVYEHA
                     LTITAWQQIYDQLHPGKFHGEFTEILLDDIQVFREYTGLALRQSCLVWPNSFWFGIPA
                     TRGEQGFIGSQCLGSAEIATRPGGTEFELSTPDDYTILGVVLSEDVITRQANFLHNPD
                     RVLHMLRSQSALEVKEQHKAALWGFVQQALATFCENPENLHQPAVRKVLGDNLLMAMG
                     AMLEEAQPMVTAESISHQSYRRLLSRAREYVLENMSEPVTVLELCNQLHVSRRTLQNA
                     FHAILGIGPNAWLKRIRLNAVRRELISPWSQSTTVKDAAMQWGFWHLGQFATDYQQLF
                     AEKPSLTLHQRMREWG"
     gene            complement(2789..3289)
                     /gene="eutK"
                     /locus_tag="PN977_004665"
     CDS             complement(2789..3289)
                     /gene="eutK"
                     /locus_tag="PN977_004665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311336.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutK"
                     /protein_id="HDI6044602.1"
                     /translation="MINALGLLEVDGMVAAIDAADAILKAANVRLLSHEVLDPGRLTL
                     VVEGDLAACRAALDAGCAAAMRTGRVISRKEIGRPDDDTQWLVTGFNRQPKQPVKEPD
                     APVIVAESADELLALLTSVRQGMTAGEVAAHFGWPLEKARNALEQLFSAGTLRKRSSR
                     YRLKPH"
     gene            complement(3302..3961)
                     /gene="eutL"
                     /locus_tag="PN977_004666"
     CDS             complement(3302..3961)
                     /gene="eutL"
                     /locus_tag="PN977_004666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutL"
                     /protein_id="HDI6044603.1"
                     /translation="MPALDLIRPSVTAMRVIASVNAEFARELKLPPHIRSLGLISADS
                     DDVTYIAADEATKQAMVEVVYGRSLYAGAAHGPSPTAGEVLIMLGGPNPAEVRAGLDA
                     MVAHIENGAAFQWANDAENTAFLAHVVSRTGSYLSSTAGITPGDPMAYLVAPPLEATY
                     GIDAALKSADVQLVTYVPPPSETNYSAAFLTGSQAACKAACNAFTDAVLEIARNPIQR
                     A"
     gene            complement(3971..4858)
                     /gene="eutC"
                     /locus_tag="PN977_004667"
     CDS             complement(3971..4858)
                     /gene="eutC"
                     /locus_tag="PN977_004667"
                     /EC_number="4.3.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit beta"
                     /protein_id="HDI6044604.1"
                     /translation="MDQKQIEEIVRSVMASMGQAAPAPSEAKCATTTCTTPVTSESCA
                     LDLGSAEAKAWIGVENPHRADVLTELRRSTVARVCTGRAGPRPRTQALLRFLADHSRS
                     KDTVLKEVPEEWVKAQGLLEVRSEISDKNLYLTRPDMGRRLCAEAVEALKAQCVANPD
                     VQVVISDGLSTDAITVNYEEILPPLMAGLKQAGLKVGTPFFVRYGRVKIEDQIGEILG
                     AKVVILLVGERPGLGQSESLSCYAVYSPRIATTVEADRTCISNIHQGGTPPVEAAAVI
                     VDLAKRMLEQKASGINMTR"
     gene            complement(4879..6240)
                     /gene="eutB"
                     /locus_tag="PN977_004668"
     CDS             complement(4879..6240)
                     /gene="eutB"
                     /locus_tag="PN977_004668"
                     /EC_number="4.3.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit alpha"
                     /protein_id="HDI6044605.1"
                     /translation="MKLKTTLFGNVYQFKDVKEVLAKANELRSGDVLAGVAAASSQER
                     VAAKQVLSEMTVADIRNNPVIAYEDDCVTRLIQDDVNETAYNQIKNWSISELREYVLS
                     DETSVDDIAFTRKGLTSEVVAAVAKICSNADLIYGAKKMPVIKKANTTIGIPGTFSAR
                     LQPNDTRDDVQSIAAQIYEGLSFGVGDAVIGVNPVTDDVENLSRVLDTIYGVIDKFNI
                     PTQGCVLAHVTTQIEAIRRGAPGGLIFQSICGSEKGLKEFGVELAMLDEARAVGAEFN
                     RIAGENCLYFETGQGSALSAGANFGADQVTMEARNYGLARHYDPFIVNTVVGFIGPEY
                     LYNDRQIIRAGLEDHFMGKLSGISMGCDCCYTNHADADQNLNENLMILLATAGCNYIM
                     GMPLGDDIMLNYQTTAFHDTATVRQLLNLRPSPEFERWLESMGIMANGRLTKRAGDPS
                     LFF"
     gene            complement(6252..7655)
                     /gene="eutA"
                     /locus_tag="PN977_004669"
     CDS             complement(6252..7655)
                     /gene="eutA"
                     /locus_tag="PN977_004669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase reactivating factor
                     EutA"
                     /protein_id="HDI6044606.1"
                     /translation="MNTRQLLSVGIDIGTTTTQVIFSHLELVNRAAVSQVPRYEFIKR
                     EISWQSPVFFTPVDKQGGLKEAELKTLILDQYQAAGIAPESVDSGAIIITGESAKTRN
                     ARPAVMTLSQSLGDFVVASAGPHLESVIAGHGAGAQTLSEQRLCRVLNIDIGGGTANY
                     ALFDAGKISGTACLNVGGRLLETDSQGRVVYAHKPGQMIVDECFGAGTDARSLTGAQL
                     VQVTRRMAELIVEVIDGTLSPLAQALMQTGLLPVGVTPEIITLSGGVGECYRHQPADP
                     FCFADIGPLLATALHDHPRLREMNVQFPAQTVRATVIGAGAHTLSLSGSTIWLEGVQL
                     PLRNLPVAIPIDEKDLVSAWQQALIQLDLDPKTDAYVLALPASLPVRYAAVLTVINAL
                     VDFVARFPNPHPLLVVAGQDFGKALGMLLRPQLQQLPLAVIDEVIVRAGDYIDIGTPL
                     FGGSVVPVTVKSLAFPS"
     gene            complement(7652..>8523)
                     /gene="eutH"
                     /locus_tag="PN977_004670"
     CDS             complement(7652..>8523)
                     /gene="eutH"
                     /locus_tag="PN977_004670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ethanolamine utilization protein EutH"
                     /protein_id="HDI6044607.1"
                     /translation="WLYSGLILGSMMGPTIVFSIPVALGIIEPSDRRYLALGVLAGIV
                     TIPIGCIAGGLVAMYSGVQINGQPVEFTFALILMNMIPVLIVAVLVALGLKFIPEKMI
                     NGFQIFAKFLVALITLGLAAAVVKFLLGWELIPGLDPIFMAPGDKPGEVMRAIEVIGS
                     ISCVLLGAYPMVLLLTRWFEKPLMSVGKVLNMNNIAAAGMVATLANNIPMFGMMKQMD
                     TRGKVINCAFAVSAAFALGDHLGFAAANMNAMIFPMIVGKLIGGVTAIGVAMMLVPKE
                     DASAAKTEAEAQS"
BASE COUNT         1927 a         2458 c         2278 g         1860 t
ORIGIN      
        1 cgcaagccat cgccaaagaa gaacctttaa aaaccagcaa cggacacagc aagccgaaag
       61 caaaaaaaaa cggcggcaaa cgtgtcgttg ttctcgatcc aggccacggc gggattgata
      121 ccggagcgat cggacgcaac ggttcgaaag aaaaacatgt ggtgctggcg attgctaaaa
      181 acgtccgttc cattttgcgt aatcatggca ttgatgcgcg tttaacgcgt tctggcgata
      241 cgtttatccc gctgtacgat cgcgttgaaa tcgcccataa acacggcgca gacctgttta
      301 tgtcaattca tgccgacggc tttactaacc cgaaagctgc cggtgcttcg gtatttgctc
      361 tctctaaccg cggcgcaagt agcgcaatgg cgaaatacct gtctgaacgc gaaaaccgcg
      421 ccgatgaagt tgccggtaaa aaggcgactg acaaggatca cctattgcag caagtgctgt
      481 ttgatctggt gcaaaccgat actattaaaa acagcctgac gctcggctcg catattctga
      541 aaaagattaa gccggtgcat aaactgcaca gccgcaacac cgaacaagcc gcgtttgtgg
      601 tgttgaaatc gccgtcggtt ccgtcggtgc tggtggaaac ctcgtttatc accaacccgg
      661 aagaagagcg gctgttaggc actgcagcgt ttcgccagaa aatcgccaca gcgattgccg
      721 aaggcgtgat cagttatttc cactggtttg acaaccagaa agcacactcg agaaagcggt
      781 aagtgatgaa acccgacgca caccaggtta aacagtttct gctcaacctt caggatacga
      841 tttgtcagca gttgagcgcc gtcgatggcg cagaatttgt cgaagatagc tggctgcgcg
      901 aagctggcgg cggcgggcgt agtcgggttt tgcgtaatgg tggtgttttc gaacaggcag
      961 gcgtcaactt ttcacatgtc cacggtgagg tgatgcctgc ttccgccacc gctcatcgtc
     1021 cggaacttgc cgggcgcagt ttcgaggcga tgggcgtttc actggtagtg catccgcata
     1081 atccgtatgt tcccaccagc cacgcgaatg tgcgcttttt tattgccgaa aaaccgggtg
     1141 cagagcccgt ctggtggttt ggtggcggct tcgatttaac ccctttctat ggttttgaag
     1201 aagacgccat tcactggcac cgcaccgccc gtgacctgtg ccagccattt ggtgaagacg
     1261 tttatccgcg ttacaaaaag tggtgcgacg aatacttcta cctcaaacat cgtaacgaac
     1321 aacgcggcat tggcggcctg ttctttgatg acctgaacac gccagacttc gaccactgtt
     1381 ttgcctttat gcaggcggta ggcaaaggct acaccaacgc ttatttacca attgtagagc
     1441 gacgtaaaac gatggcctac ggcgagcgcg agcgcaattt ccagttatat cgtcgcggtc
     1501 gttatgtcga gttcaatctg gtctgggatc gtggcacact gtttggcctg caaaccggcg
     1561 ggcgcaccga gtctatcctg atgtcaatgc cgccactggt acgctgggag tatgattatc
     1621 agccaaaaga tggcagcccg gaagcggcgt taaatgagtt tattaaggtc agggattggg
     1681 tgtaactccc tcacccccac tcccgcatcc gctgatgcag cgtcagtgac ggcttctcgg
     1741 caaacagctg ctggtaatcc gtggcaaatt gccccagatg ccagaatccc cactgcatgg
     1801 cggcgtcttt taccgttgtg ctttgcgacc acgggcttat cagttcgcgg cgtacggcgt
     1861 tcaggcgaat gcgtttcagc cacgcgtttg ggccaatgcc taaaatagcg tgaaacgcgt
     1921 tttgtagcgt gcggcggctg acatgcaatt gattgcacaa ctccaacacc gttaccggtt
     1981 cagacatgtt ttccagcaca tattcacggg cgcgggaaag caatcgacgg taactctgat
     2041 gactgatgct ttccgccgtc accattggct gcgcttcttc cagcatcgcc cccatcgcca
     2101 tgagcaaatt atcccccagc actttacgca cagcaggctg atgaagattt tccgggttct
     2161 cgcaaaatgt cgccagcgcc tgttggacaa agccccacag cgcggcttta tgctgctctt
     2221 tcacttccaa cgccgactgg ctacgcagca tatgtaatac ccgatccgga ttatgcaaaa
     2281 agttagcctg acgagtgatg acatcttcag aaagcactac gcccaggatc gtgtaatcgt
     2341 ccggcgtact taattcaaac tcggttccac ccgggcgcgt cgcaatttct gcgcttccca
     2401 gacattgcga accgataaat ccctgctcac cgcgcgtcgc cggaatgcca aaccagaacg
     2461 agttcggcca gaccaggcac gactgacgca gcgccagacc agtgtattca cgaaaaacct
     2521 gaatatcatc gagtagaatt tccgtaaatt caccatgaaa cttgcccgga tgcagctgat
     2581 cgtaaatctg ctgccaggcg gtaattgtca aagcatgttc atagacatcc gttgtctgtc
     2641 gttgatgaac attatccact tcgaccttcg gcgtgagctt caggttttcg ggtaagggtt
     2701 catgataaag atggtgcaaa ttggctgtac gggtcttttt catgatgtta atgccgggtg
     2761 ttgtaggaca cccggcacct ccgacaggtt aatggggctt gagacggtaa cgactactgc
     2821 gtttacgtaa cgtcccggca gaaaagagct gttcgagcgc atttctggct ttttccagcg
     2881 gccagccaaa gtgggcagcc acttctcctg ccgtcattcc ctgacgtact gatgttaaca
     2941 gcgccaacaa ctcgtcagca gattccgcga cgataactgg cgcgtcgggt tcctttacgg
     3001 gttgcttcgg ctggcggtta aagccagtaa ccagccactg ggtgtcatcg tctggccgac
     3061 cgatctcctt acggctgatg acacgcccgg tacgcatcgc ggcagcacaa cctgcgtcca
     3121 gcgccgcacg acacgccgcc agatcgcctt ccaccaccag cgttaggcga ccagggtcaa
     3181 gcacttcatg actgagcaga cgaacgttag ctgctttgag tatggcatca gctgcatcta
     3241 tcgcggcgac catgccgtcc acttccagca atcccagtgc attgatcatc ggcaacctcc
     3301 gttacgcacg ctggattgga ttacgcgcga tttccagcac tgcatcggta aaggcgttac
     3361 aggctgcttt acacgcggcc tggctaccgg ttaaaaatgc tgccgagtag ttggtttcag
     3421 acggtggcgg gacataggtc accagctgca cgtcggcaga tttcaacgct gcatcaatgc
     3481 cgtaggtcgc ttccagcggc ggtgccacca gatacgccat cggatcgcca ggcgtgatcc
     3541 cggcggttga tgagagataa gaaccggtac gcgaaacgac atgtgccagg aaggctgtat
     3601 tttctgcgtc attagcccac tggaaagcag cgccattttc aatatgcgca accatcgcat
     3661 ccagaccagc acgcacttcc gccgggtttg gcccacccag cataatcagc acctcaccgg
     3721 cagtcggtga cgggccgtgt gcagcgcccg catacagcga gcgaccatat accacttcga
     3781 ccatcgcctg cttggtcgct tcgtcagccg caatgtaggt gacgtcatct gaatcagcag
     3841 aaatcagccc gaggctacga atatgcggcg gcaatttcag ttcacgtgca aattcggcgt
     3901 taacggaggc aatcacccgc atggcggtta ccgacggtcg aatcaaatct aaagctggca
     3961 tgatgcctcc ttatcgggtc atgttgatgc cggatgcttt ctgctccagc atacgtttcg
     4021 ccaaatccac aatgacggct gcggcttcaa ccggcggcgt gccgccctgg tgaatgttag
     4081 agatacaggt acgatcggcc tcaacggtgg ttgcaatacg cggcgagtaa acggcgtagc
     4141 aggagaggct ttctgactgc cctaatcccg ggcgttcccc caccagcagg atcaccactt
     4201 tcgcgccgag gatctcaccg atctgatctt caatcttcac gcgaccataa cgaacaaaga
     4261 atggcgtacc gactttcagc ccggcctgtt tcaggcccgc catcagcggt ggcaggatct
     4321 cttcgtagtt cacggtgatc gcatcagttg acaggccatc agaaataacg acctgcacgt
     4381 ccggattggc aacacactgc gctttcagcg cttcaacagc ttctgcacac aggcggcggc
     4441 ccatatccgg acgagtcagg tagagatttt tgtcgctaat ttcagagcgg acttccagca
     4501 agccctgcgc tttcacccac tcttccggca cttctttcag tacggtatct ttcgaacgag
     4561 agtgatcggc caggaaacgc agcaacgcct gggtacgcgg acgcggaccg gcacgaccgg
     4621 tacaaacgcg ggccacggtg ctgcgacgca gttctgttaa tacgtctgcg cgatgcgggt
     4681 tttccacacc aatccacgct tttgcttccg cagaacctaa atccagcgcg cagctttccg
     4741 aggtcactgg tgtcgtacag gtggtggtgg cgcactttgc ttctgacggc gcgggggccg
     4801 cttgtcccat tgacgccatc acgctgcgta caatttcttc aatctgtttt tgatccatga
     4861 tgtgttatct ccgcgtcatc agaagaacag tgacggatcg cccgcccgtt tggtcaggcg
     4921 accgtttgcc ataatgccca tgctttccag ccagcgttca aactccggtg acgggcgcag
     4981 gttgagtaac tgacgcacag tggcggtgtc gtggaaggcc gtagtctggt agttgagcat
     5041 gatgtcatca cccagcggca tccccatgat gtagttacag cctgcggtgg cgagcaggat
     5101 catcaggttt tcgttgaggt tctggtcagc gtcagcgtgg ttggtgtagc agcagtcaca
     5161 gcccatagag atgccgctca gcttgcccat aaagtgatct tctaagcccg cgcggataat
     5221 ctggcggtcg ttgtagagat actccggccc aataaaaccg accacggtgt tgacgataaa
     5281 cggatcgtaa tgacgcgcca gcccatagtt acgcgcttcc atcgtcacct ggtcagcgcc
     5341 gaagttagcg ccagcggaca gcgccgaacc ttgtccggtt tcgaagtaga ggcagttttc
     5401 cccggcgata cggttaaact ctgcacccac tgcgcgcgct tcgtcgagca tcgccagctc
     5461 cacgccaaac tctttcagcc ctttttcgct gccgcaaata ctctggaaaa tcagcccgcc
     5521 cggtgcgccg cgacgaatcg cttcgatctg ggtggtgacg tgcgccagta cgcagccctg
     5581 agttgggatg ttgaatttgt cgatcacgcc atagatggta tccaacacgc ggcttaagtt
     5641 ttccacgtcg tcagtcaccg ggttaacgcc gatcaccgca tcgcccaccc cgaaggaaag
     5701 cccttcgtag atttgcgcgg cgatactttg cacatcgtca cgggtatcgt ttggctgcaa
     5761 gcgggcgcta aaggtgcccg gaataccgat ggtggtattg gcctttttga tcaccggcat
     5821 tttcttcgcg ccgtagatca ggtccgcgtt ggagcaaatc ttcgctaccg ccgctaccac
     5881 ttccgaggtc agccctttgc gggtaaaggc aatgtcgtcc acgctggttt catcgctcag
     5941 cacatactca cgcagttcgc tgatgctcca gtttttaatc tggttgtagg ccgtttcgtt
     6001 aacatcgtcc tgaatcagcc gcgtcacgca gtcatcttca taggcaatca ccggattatt
     6061 gcggatgtcc gctacggtca tttccgacaa cacctgcttt gccgccacgc gctcctgtga
     6121 gcttgccgca gcaacgcccg ccagcacatc ccccgaacgc agttcgttgg ctttagccag
     6181 cacctctttt acatccttaa actgatatac attgccgaac aatgtggtct ttagtttcat
     6241 aagtcgttcc ctcaggaagg aaatgcgagt gatttcaccg tcaccggcac aaccgatccg
     6301 ccaaaaagag gcgtaccaat gtcgatatag tcccccgcgc ggacaatcac ttcgtcaatg
     6361 actgccaacg ggagttgttg tagctgtggg cgcaacaaca tgcccagagc tttaccaaag
     6421 tcctgcccgg ccaccaccag caggggatgc ggattcggaa aacgcgcgac gaaatcgacc
     6481 aacgcgttga tgaccgtcag taccgcggcg taacgcacag gcagcgaggc gggaagcgcc
     6541 agcacgtacg cgtcagtttt aggatcaaga tccagctgaa tcagcgcctg ttgccaggca
     6601 ctcaccagat ccttttcatc aattgggatc gccaccggca agttgcgcag cggcagttgt
     6661 acgccctcca gccagattgt gctgccagag agcgaaaggg tatgtgcacc cgcgccaatc
     6721 accgtggcgc gcacggtttg cgccggaaac tgcacgttca tctcacgcag gcgcggatgg
     6781 tcatgcagcg ccgttgccag aagcgggcca atatcggcaa aacagaacgg gtcggcgggc
     6841 tggtggcgat aacattcgcc aacgccgcca gaaagcgtaa tgatttcggg cgtaacacct
     6901 acgggcagca aaccggtttg catcaatgcc tgcgcgagcg gcgaaagcgt tccgtcaatc
     6961 acttcgacaa tcagctctgc catccggcgg gtaacctgca ccagctgcgc gccggtcagc
     7021 gaacgggcgt cagtgcctgc gccgaagcac tcatccacaa tcatctgccc cggtttatga
     7081 gcgtaaacca cgcgcccctg gctgtcggtt tccagcaggc gaccgccgac gttgaggcag
     7141 gcggtgccgc tgatttttcc ggcatcgaac agggcgtagt tcgcagtgcc accgccgata
     7201 tcgatattca gtacccgaca cagccgttgt tcagaaagag tttgcgcccc ggcaccgtga
     7261 ccggcgatca cggattcgag atgcggcccg gcgctggcga cgacaaaatc gcccagcgac
     7321 tgggagagcg tcatcaccgc cgggcgagcg ttgcgggttt tcgcgctttc accggtgatg
     7381 atgatggcac cagaatcaac actttccggc gcaatgcccg cagcctgata ttgatccagg
     7441 attaaggttt tcagttccgc ttcttttaaa ccaccctgtt tatcgacagg ggtaaagaac
     7501 accgggcttt gccagctaat ttcgcgtttg atgaattcgt agcgcggcac ctgcgacacc
     7561 gccgcacggt taaccagctc caggtgggag aaaatcacct gggtggtggt ggtgccgata
     7621 tcgataccga cgctcagtag ctggcgagtg ttcacgattg cgcctccgct tcggttttag
     7681 ccgcgctcgc gtcttcttta ggtaccagca tcatcgccac gccaatcgcc gtgacgccgc
     7741 cgatcagctt gccgacaatc atcgggaaga tcatggcgtt catgttggca gccgcgaagc
     7801 ctaaatggtc gcccagggcg aaagcagcgg aaacggcgaa ggcacagttg atgactttgc
     7861 cgcgggtatc catctgcttc atcatgccaa acatcgggat gttgttggca agcgttgcca
     7921 ccatgccggc tgccgctata ttgttcatat tcagcacctt accgacactc atcagcggtt
     7981 tttcaaacca gcgagtcagc agcagcacca tcggatacgc ccctaacaga acgcaggaga
     8041 tcgagccgat aacttcaatg gcgcgcatca cttcaccggg tttatcgcca ggggccataa
     8101 agataggatc aaggcccggg atcagttccc agccaaggag gaatttcact accgcagcgg
     8161 caagaccgag ggtgatcaat gcaacgagga atttggcgaa gatctggaag ccgttgatca
     8221 ttttttccgg gatgaatttc agccccagcg ccaccagcac cgcaacgata agtaccggga
     8281 tcatgttcat caggatcagc gcaaaggtga attccactgg ctgaccgttg atctgcacac
     8341 cggagtacat agccaccaga ccaccggcaa tacagccaat cggaatggtc acaatgcccg
     8401 ccagcacgcc gagcgccaga taacgacggt cagaaggttc gataatgccg agcgccaccg
     8461 gaatggaaaa cacaatcgtt ggccccatca tcgacccgag aattaatcca gagtatagcc
     8521 acg
//
LOCUS       DAMZFT010000192         8441 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.192, whole genome shotgun sequence.
ACCESSION   DAMZFT010000192 DAMZFT010000000
VERSION     DAMZFT010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8441)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8441)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8441
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.192"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            60..671
                     /gene="ruvA"
                     /locus_tag="PN977_004671"
     CDS             60..671
                     /gene="ruvA"
                     /locus_tag="PN977_004671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012131997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HDI6044608.1"
                     /translation="MIGRLRGIIIEKQPPLVLIEVGGVGYEVHMPMTCFYELPEAGQE
                     AIVFTHFVVREDAQLLYGFNNKQERTLFKELIKTNGVGPKLALAILSGMSAQQFVNAV
                     EREEVGALVKLPGIGKKTAERLIVEMKDRFKGLHGDLFTPAADLVLTSPASPATDDAE
                     QEAVAALVALGYKPQEASRMVSKIARPDASSETLIREALRAAL"
     gene            680..1690
                     /gene="ruvB"
                     /locus_tag="PN977_004672"
     CDS             680..1690
                     /gene="ruvB"
                     /locus_tag="PN977_004672"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HDI6044609.1"
                     /translation="MIEADRLISAGTTLPEDVADRAIRPKLLEEYVGQPQVRSQMEIF
                     IKAAKLRGDALDHLLIFGPPGLGKTTLANIVANEMGVNLRTTSGPVLEKAGDLAAMLT
                     NLEPHDVLFIDEIHRLSPVVEEVLYPAMEDYQLDIMIGEGPAARSIKIDLPPFTLIGA
                     TTRAGSLTSPLRDRFGIVQRLEFYQVPDLQYIVSRSARFMGLEMSDDGALEVARRARG
                     TPRIANRLLRRVRDFAEVKHDGTISADIAAQALDMLNVDAEGFDYMDRKLLLAVIDKF
                     FGGPVGLDNLAAAIGEERETIEDVLEPYLIQQGFLQRTPRGRMATVRAWNHFGITPPE
                     MP"
     gene            complement(1837..2622)
                     /gene="znuB"
                     /locus_tag="PN977_004673"
     CDS             complement(1837..2622)
                     /gene="znuB"
                     /locus_tag="PN977_004673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter permease subunit ZnuB"
                     /protein_id="HDI6044610.1"
                     /translation="MIELLFPGWLAGIMLACAAGPLGSFVVWRRMSYFGDTLAHASLL
                     GVAFGLLLDVNPFYAVIAVTLLLAGGLVWLEKRPQLAIDTLLGIMAHSALSLGLVVVS
                     LMSNIRVDLMAYLFGDLLAVTPEDLISIAIGVVIVVAILFWQWRNLLSMTISPDLAFV
                     DGVKLQRVKLLLMLVTALTIGVAMKFVGALIITSLLIIPAATARRFARTPEQMAGVAV
                     LVGMVAVTGGLTFSAFYDTPAGPSVVLCAALLFIISMMKKQAS"
     gene            complement(2619..3374)
                     /gene="znuC"
                     /locus_tag="PN977_004674"
     CDS             complement(2619..3374)
                     /gene="znuC"
                     /locus_tag="PN977_004674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter ATP-binding protein ZnuC"
                     /protein_id="HDI6044611.1"
                     /translation="MTSLVSLENVSVSFGQRRVLSDVSLELKPGKILTLLGPNGAGKS
                     TLVRVVLGLVTPDEGVIKRNGKLRIGYVPQKLYLDTTLPLTVNRFLRLRPGTHKEDIL
                     PALKRVQAGHLINAPMQKLSGGETQRVLLARALLNRPQLLVLDEPTQGVDVNGQVALY
                     DLIDQLRRELDCGVLMVSHDLHLVMAKTDEVLCLNHHICCSGTPEVVSLHPEFISMFG
                     PRGAEQLGIYRHHHNHRHDLQGRIVLRRGNDRS"
     gene            3453..4385
                     /gene="znuA"
                     /locus_tag="PN977_004675"
     CDS             3453..4385
                     /gene="znuA"
                     /locus_tag="PN977_004675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     ZnuA"
                     /protein_id="HDI6044612.1"
                     /translation="MLHKKTLLFAALSAALWGGATQAADAAVVASLKPVGFIASAIAD
                     GVTETEVLLPDGASEHDYSLRPSDVKRLQNADLVVWVGPEMEAFMQKPVSKLPEAKQV
                     TIAQLENVKPLLMKSIHGDDDDHDHAEKSDEDHHHGDFNMHLWLSPEIARATAVAIHE
                     KLVELMPQSRAKLDANLKDFEAQLASTETQVGHELAPLKGKGYFVFHDAYGYFEKQFG
                     LTPLGHFTVNPEIQPGAQRLHEIRTQLVEQKATCVFAEPQFRPAVVESVARGTSVRMG
                     TLDPLGTNIKLGKTSYSEFLNQLANQYASCLKGD"
     gene            4401..5723
                     /gene="mepM"
                     /locus_tag="PN977_004676"
     CDS             4401..5723
                     /gene="mepM"
                     /locus_tag="PN977_004676"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein DD-endopeptidase MepM"
                     /protein_id="HDI6044613.1"
                     /translation="MQQIARSVALAFNNLPRPHRVMLGSLTVLTLAVAVWRPYVYHRD
                     ATPIVKTIELEQNEIRSLLPEASEPIDQAAQEDEAIPQDELDDKIAGEAGVHEYVVST
                     GDTLSSILNQYGIDMGDITQLAAADKELRNLKIGQQLSWTLTADGELQRLTWEVSRRE
                     TRTYDRTAANGFKMTSEMQQGEWVNNLLKGTVGGSFVASARNAGLTSAEVSAVIKAMQ
                     WQMDFRKLKKGDEFAVLMSREMLDGKREQSQLLGVRLRSEGKDYYAIRAEDGKFYDRN
                     GTGLAKGFLRFPTAKQFRISSNFNPRRTNPVTGRVAPHRGVDFAMPQGTPVLSVGDGE
                     VVVAKRSGAAGYYVAIRHGRSYTTRYMHLRKILVKPGQKVKRGDRIALSGNTGRSTGP
                     HLHYEVWINQQAVNPLTAKLPRTEGLTGSDRREFLAQAKEIVPQLRFD"
     gene            5842..6813
                     /gene="lpxM"
                     /locus_tag="PN977_004677"
                     /gene_synonym="msbB"
     CDS             5842..6813
                     /gene="lpxM"
                     /locus_tag="PN977_004677"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416369.1"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HDI6044614.1"
                     /translation="METKKNNSEYIPEFDKSFRHPRYWGAWLGVAAMAGIALTPPKLR
                     DPILARLGRFAGRLGKSSRRRALINLSLCFPERSEAEREAIVDEMFATAPQAMAMMAE
                     LAIRGPEKIQPRVEWQGLEIIEEMRRNNEKVIFLVPHGWAVDIPAMLMASQGQKMAAM
                     FHNQGNPVFDYVWNTVRRRFGGRLHARNDGIKPFIQSVRQGYWGYYLPDQDHGPEHSE
                     FVDFFATYKATLPAIGRLMKVCRARVVPLFPIYDGKTHRLTIQVRPPMDDLLEADDHT
                     IARRMNEEVEIFVGPRPEQYTWILKLLKTRKPGEIQPYKRKDLYPIK"
     gene            complement(6945..8387)
                     /gene="pyk"
                     /locus_tag="PN977_004678"
     CDS             complement(6945..8387)
                     /gene="pyk"
                     /locus_tag="PN977_004678"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HDI6044615.1"
                     /translation="MSRRLRRTKIVTTLGPATDRDNNLEKVIAAGANVVRMNFSHGSP
                     EDHKMRADKVREIAAKLGRHVAILGDLQGPKIRVSTFKEGKIFLNIGDKFLLDANLGK
                     GEGDKEKVGIDYKGLPADVVPGDILLLDDGRVQLKVLEVQGMKVFTEVTVGGPLSNNK
                     GINKLGGGLSAEALTEKDKADIKTAALIGVDYLAVSFPRCGEDLNYARRLARDAGCDA
                     KIVAKVERAEAVCSQDAMDDIILASDVVMVARGDLGVEIGDPELVGIQKALIRRARQL
                     NRAVITATQMMESMITNPMPTRAEVMDVANAVLDGTDAVMLSAETAAGQYPSETVAAM
                     ARVCLGAEKIPSINVSKHRLDVQFDNVEEAIAMSAMYAANHLKGVTAIITMTESGRTA
                     LMTSRISSGLPIFAMSRHERTLNLTALYRGVTPVHFDSANDGVAAASEAVNLLRDKGY
                     LMSGDLVIVTQGDVMSTVGSTNTTRILTVE"
BASE COUNT         2147 a         2135 c         2191 g         1968 t
ORIGIN      
        1 ctatccagca tttttttatc atacagcatt atctttgatt cattacgcag gagcgtcatg
       61 tgataggcag actcagaggc atcatcattg aaaaacaacc cccgctggtg ttaattgaag
      121 tgggcggcgt aggctatgaa gtgcatatgc cgatgacctg tttttatgaa ctccctgaag
      181 cgggtcagga agcgatcgtt ttcacccact ttgtggtgcg tgaagacgcg caactgctgt
      241 acggttttaa caataaacaa gagcgcacgt tgttcaaaga gttgatcaaa accaacggcg
      301 tcggcccgaa gttggcgctg gcgatcctct ctggaatgtc agcgcagcag ttcgttaatg
      361 ccgttgagcg tgaagaagtg ggggcgctgg tgaaactgcc gggtattggc aaaaaaaccg
      421 ccgaacgctt gattgttgaa atgaaagacc gatttaaagg tttgcatggc gatctcttta
      481 cgccagctgc cgacctggta ctcacgtcac ctgccagccc ggcgaccgac gatgctgaac
      541 aggaagctgt tgccgcgctg gtggcgctgg gctataaacc acaagaagca agccgcatgg
      601 tgagcaaaat cgctcgccct gacgccagca gtgaaacatt aattcgcgaa gccctacgcg
      661 ccgcgttatg aggtaaagga tgattgaagc agaccgtctg atttctgccg gtaccacttt
      721 gccggaagat gtggcagatc gcgccattcg ccctaaatta ctggaagagt atgttggtca
      781 accgcaggtt cgttcacaga tggagatttt catcaaagca gcgaaactgc gtggcgatgc
      841 cctcgatcat ctgttgattt ttggtcctcc ggggttgggt aaaactacgc ttgctaacat
      901 tgtcgccaat gaaatgggcg ttaatttacg cacgacttct ggtccggtgc tggaaaaggc
      961 gggggatctg gcagcgatgc tcactaacct tgaaccgcat gacgtgctgt ttattgatga
     1021 gatccaccgt ctatcgccag ttgttgaaga agtgctgtac ccggcaatgg aagactacca
     1081 actggatatt atgattggtg aaggtccggc ggcacgctcc attaaaattg atttgccgcc
     1141 gtttaccctg attggtgcaa ccacgcgcgc aggttcgctg acatcaccgt tgcgcgatcg
     1201 ttttggtatt gtgcagcgtc tggagtttta tcaggtgccg gatctgcaat atatcgtcag
     1261 tcgcagcgca cgctttatgg ggcttgagat gagtgatgac ggcgcgctgg aagttgctcg
     1321 tcgcgctcgc ggtacgccgc gcattgctaa ccgtctgctg cgtcgagtgc gtgatttcgc
     1381 cgaagtgaag cacgatggca ccatctcggc tgatatcgct gctcaggcgc tggatatgct
     1441 gaatgtcgat gccgaaggtt tcgattatat ggaccgcaaa ttgttgctgg cggtaatcga
     1501 taagttcttt ggtgggccgg taggtctgga taacctggcg gctgccattg gtgaagaacg
     1561 tgaaaccatt gaggatgtgc tggaacctta tttgattcag caaggctttt tgcagcgtac
     1621 accgcgcggg cgtatggcga cagtgcgggc gtggaaccac ttcggcatca cgccaccaga
     1681 aatgccgtaa gtcggattgg ccgctcgagc agcatccgac actggcagtg tgcctgatgc
     1741 gacgctgttc gcgtcttatc aggcctacca gagatagcga accgtaggtg ggataaggcg
     1801 ctcgcgccgc atccgacaaa tgtgttcagc gacagattag ctggcctgct ttttcatcat
     1861 actgataata aataacagtg ccgcacatag cactaccgac ggacccgccg gcgtatcgta
     1921 aaatgcggaa aaggttaaac cgccagttac tgccaccatt cccaccaaaa cagcgacacc
     1981 ggccatctgt tccggcgtgc gggcaaagcg acgcgctgta gcagcaggaa taatcagcaa
     2041 cgaagtaata atcaacgcgc cgacaaattt catcgctaca ccaatcgtca atgccgtcac
     2101 cagcatcaac aacaatttca cgcgctgtaa tttcacacca tcaacaaacg ccagatccgg
     2161 gctaatcgtc attgacagca aattgcgcca ttgccagaac agaatcgcca ccacgatgac
     2221 cacgccaatc gcaatagaga tgagatcttc tggcgtcact gccagcaaat caccgaacag
     2281 gtaagccatc aaatcaacac gaatgttaga catcagacta acgaccacca ggcccagcga
     2341 cagggcactg tgcgccataa tccctaataa cgtgtcgatc gccagctgtg gacgcttctc
     2401 cagccatacc agaccgcccg ccagcagcag cgtaacggca atcaccgcat agaatggatt
     2461 cacgtccagc aacaaaccaa acgcgacgcc aagtaatgag gcatgagcca gcgtatcacc
     2521 gaaataagac atacgacgcc agactacaaa cgaacccagc ggacccgcgg cacaggcgag
     2581 catgatcccg gctaaccaac caggaaataa taattcaatc atgagcgatc atttccccga
     2641 cgcaagacaa ttcgtccctg taaatcgtga cgatgattat gatgatggcg atagataccc
     2701 agttgttcag caccacgagg accaaacata gaaataaact ccggatgcag ggaaacaact
     2761 tccggtgtgc cggaacaaca aatgtggtga ttcaggcaaa gcacttcatc ggtttttgcc
     2821 attaccagat gcagatcgtg agataccatt aaaacgccac aatccagttc gcgacgcagt
     2881 tggtcaataa ggtcatataa cgccacctga ccattcacat ccacgccctg agtgggttca
     2941 tccagcacta ataattgcgg tcgatttaac aatgctcgcg ctaacagtac acgctgcgtt
     3001 tcgccacccg agagcttttg catcggtgcg ttaatcaggt gtccggcctg gacacgtttc
     3061 agtgcaggca aaatatcttc tttatgtgtg ccaggtcgta agcgtaaaaa acggtttacg
     3121 gtcagtggca acgtggtgtc gagatacagc ttctgcggta catagccgat gcgtagtttt
     3181 ccgttgcgct tgataacccc ttcatcgggt gttaccagcc cgagcactac ccgtaccagt
     3241 gtcgacttac ctgcgccatt tggcccaagt aaagtcaaaa tttttccagg tttaagttcc
     3301 agcgacacat cagagaggac gcggcgttgg ccaaaagaaa ccgagacatt ttccagggaa
     3361 accagacttg tcatgttaat tttagtcttg cagtagtcat gaaatgttat aatatcacac
     3421 ttctcatatt cattacgatg attggtcgca ttatgttaca taaaaaaacg cttcttttcg
     3481 cagcattatc cgccgctctc tggggaggtg caacacaggc cgcagatgcc gccgttgtcg
     3541 cttcgcttaa acccgttggg ttcatcgctt ctgccattgc tgatggggta acagaaacgg
     3601 aggttttact acctgacggc gcttcagagc atgattattc actgcgtcca tcagatgtaa
     3661 aacgcttaca gaacgcggac ttagtcgttt gggttggccc ggagatggaa gcgttcatgc
     3721 aaaaaccggt aagtaaatta cctgaagcca aacaggtaac gattgcgcag ctagagaatg
     3781 tgaaaccgct gctgatgaaa agtattcacg gcgatgatga tgatcacgac cacgcggaaa
     3841 aaagtgacga agatcaccat cacggcgatt tcaacatgca tctttggctt tccccagaga
     3901 tagcgcgggc tacagcggtt gcaatccatg aaaaattagt ggaacttatg ccgcaaagtc
     3961 gagccaaact tgacgccaac ctgaaggatt ttgaggcaca attagcctca accgaaacgc
     4021 aggttggtca cgagctcgcg ccgctcaagg ggaaaggtta tttcgttttt cacgatgctt
     4081 acggctactt cgaaaaacag ttcggactaa caccgcttgg tcattttacc gttaaccctg
     4141 agattcaacc tggcgcgcag cgtttacatg aaataagaac acagttggtt gagcaaaaag
     4201 caacctgcgt ttttgctgag ccacagttca ggccagcggt cgttgagagc gtcgctcgag
     4261 ggacatccgt tcgtatggga acgttggatc ccctggggac gaatatcaaa ctgggtaaaa
     4321 caagctattc agaattcctg aatcaattag ccaaccagta tgcgagctgc ctgaaaggag
     4381 attaatgagg aagtgattac gtgcaacaga tagcccgctc tgtcgccctg gcgtttaata
     4441 atttaccgcg accacaccgc gttatgttgg ggtcgctcac cgttcttact ctggccgtcg
     4501 ctgtctggcg gccctatgtt tatcaccgcg acgccacgcc aattgtcaaa accattgagc
     4561 tggaacagaa cgaaattcgt tcgctcttac ctgaagccag tgagccgatt gatcaagctg
     4621 cacaagaaga tgaagccatt ccccaggatg aactggatga caaaatcgcc ggtgaagcgg
     4681 gcgtgcatga atatgttgtt tccactggcg atacgctaag cagcattctc aatcagtatg
     4741 gtattgatat gggtgatatc acccaactgg ctgctgccga caaagaattg cgtaacctga
     4801 aaatcggtca acaactctcc tggacattaa ccgcggacgg cgaactgcaa cgcctcacct
     4861 gggaagtgtc tcgtcgtgaa acccgaacct atgaccgtac tgccgctaac ggttttaaaa
     4921 tgaccagcga aatgcagcaa ggagagtggg tcaacaatct gctgaaaggt accgtcgggg
     4981 gaagctttgt tgccagcgcc agaaacgccg gtttaaccag cgccgaagtg agcgcagtga
     5041 ttaaagccat gcagtggcaa atggatttcc gcaaactgaa aaaaggcgat gaatttgcgg
     5101 tgttaatgtc acgagaaatg cttgatggta aacgtgagca aagccagctg ctgggcgtac
     5161 gtttgcgttc agaaggtaaa gattattacg caatccgcgc tgaagatggc aaattctacg
     5221 atcgtaacgg tactggtctg gcgaaaggat tcttgcgatt cccgacggcg aaacagttcc
     5281 gtatctcatc taactttaac ccgcgtcgta ctaatccggt gaccggtcgc gttgcgccac
     5341 acagaggtgt tgattttgcc atgccacaag gtacgccagt gctttcagtg ggtgacggtg
     5401 aagtggtggt tgccaaacgc agtggcgcag caggttatta tgtggctatt cgtcatggtc
     5461 gcagctacac cacgcgttat atgcacttgc gcaagatcct ggtgaaaccg ggacagaagg
     5521 tgaaacgtgg cgaccgtatc gcgctttccg gtaataccgg acgttcaacc gggccgcatc
     5581 tgcactatga agtatggata aaccagcagg ccgtaaaccc gctgacggca aaactgccgc
     5641 gtaccgaagg gctgaccggc tccgatcgtc gcgaattcct ggcacaggcc aaagagattg
     5701 tgccgcagct acggtttgat taattaacat ccattcgcag ccggtacgca gtcagtaccg
     5761 gcttttttat ttggtgcggg gcaagttgcg ccgctacact atcaccagat tgatttttgc
     5821 cttatccgaa actggaaaag catggaaacg aaaaaaaata atagcgaata cattcctgag
     5881 tttgataaat cctttcgcca cccgcgctac tggggagcat ggctgggcgt agcagcgatg
     5941 gcgggtattg ctttaacgcc gccaaagctc cgtgatccca ttctggcacg gctgggacgt
     6001 tttgccggac gactgggaaa aagctcacgc cgtcgtgcgt taatcaatct gtcgctctgc
     6061 tttccagaac gtagtgaagc tgaacgcgaa gcgattgttg atgagatgtt tgccactgcg
     6121 ccgcaagcga tggcaatgat ggctgagttg gcaatacgcg ggccggagaa aattcagccg
     6181 cgcgttgaat ggcaagggct ggagatcatc gaagagatgc ggcgtaataa cgagaaagtg
     6241 atttttctgg tgccgcacgg ttgggccgtc gatattcctg ccatgctgat ggcctcgcaa
     6301 gggcagaaaa tggcagcgat gttccataat cagggcaacc cggtttttga ttatgtctgg
     6361 aacacggtgc gtcgtcgctt tggtggtcgt ctgcatgcga gaaatgatgg tattaaacca
     6421 ttcatccagt cggtacgtca ggggtactgg ggatattatt tacccgatca ggatcatggc
     6481 ccagagcaca gcgaatttgt tgatttcttt gccacctata aagcgacgtt gcccgcgatt
     6541 ggtcgtttga tgaaagtgtg ccgtgcgcgc gttgtaccgc tgtttccgat ttatgatggc
     6601 aagacgcatc gcctgacgat tcaggtgcgc ccaccgatgg atgatctgtt agaggcggat
     6661 gaccatacga ttgcgcggcg gatgaatgaa gaagtcgaga tttttgttgg tccgcgacca
     6721 gaacaataca cctggatatt aaaattgctg aaaactcgca aaccgggcga aatccaaccg
     6781 tataagcgca aagatcttta tcccatcaaa taaaaaaagc ctctcgcgag gataggcttc
     6841 cgccgtgtga taagttcaag tttgcttcag aagattcgaa atctgttgaa ctatcattga
     6901 actgtaggcc ggatgaggcg ttttcgccgc atccggcaac gtacttactc taccgttaaa
     6961 atacgcgtgg tattagtaga acccacggta ctcatcacgt cgccctgggt gacaatcacc
     7021 aggtcaccag acatcaagta acctttatcg cgcagcagat taaccgcttc gctggcagct
     7081 gctacgccgt cattagcgct atcaaagtgc accggcgtaa cgccgcgata gagagcagtc
     7141 aggttcagcg tacgttcatg gcgtgacatg gcgaaaattg gcagaccaga gctgatacgg
     7201 gaggtcatca gcgccgtacg acccgattcg gtcatggtga tgatcgccgt aacgcctttc
     7261 aggtggttag ccgcgtacat tgctgacatg gcgatagctt cttccacatt gtcgaactga
     7321 acgtccagac ggtgtttaga aacgttgatg ctcgggattt tttccgcacc caggcaaacg
     7381 cgcgccatgg ctgcaacagt ttctgaagga tactgaccag cagcagtttc tgcagaaagc
     7441 atcacagcgt cagtaccatc cagaacggcg tttgctacgt ccatgacttc tgcacgcgtc
     7501 ggcatcgggt tagtaatcat tgactccatc atctgggtcg cagtgattac tgcgcggtta
     7561 agctgacgtg cacgacggat caacgccttc tgaatgccga ccagttccgg atcgccaatt
     7621 tccacaccga ggtcgccgcg agcaaccatt accacgtcag aggcgaggat gatgtcatcc
     7681 attgcatcct ggctgcatac ggcttccgca cgttcaacct tggcaacaat tttcgcatca
     7741 catcctgcat cgcgtgccag gcgacgggca tagttcagat cttcgccgca gcgtgggaag
     7801 gagacagcca ggtaatctac gccaattaac gccgcagtct taatgtctgc tttgtctttt
     7861 tcggtcagcg cttcagccga caaaccgccg ccaagtttgt tgataccttt attgttggag
     7921 aggggaccac cgacggtcac ttcggtgaac actttcatgc cctggacttc cagtactttt
     7981 aactggacgc gaccatcgtc cagcagcagg atgtcaccag gcacaacgtc agcaggcagg
     8041 cctttgtagt cgataccgac tttttctttg tcgccttcac ctttacccag gttggcgtcg
     8101 agcaggaatt tatccccaat attgaggaaa attttgcctt ctttaaaggt ggatacacgg
     8161 attttgggcc cctggaggtc acccagaata gccacatgac gccccagttt tgcggcaatc
     8221 tcacgaactt tatccgcgcg cattttgtga tcttcaggcg agccgtgaga aaaattcata
     8281 cgtacaacgt tggcacccgc cgcgataact ttttcaaggt tattatcgcg atctgttgcc
     8341 gggcctaacg tggtaacgat tttagttctg cgaagccttc tggacatgta atactccgtt
     8401 gactgaaaca accaggtgtt gcttgaacat gaaatccgaa t
//
LOCUS       DAMZFT010000193         8440 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.193, whole genome shotgun sequence.
ACCESSION   DAMZFT010000193 DAMZFT010000000
VERSION     DAMZFT010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8440)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8440)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8440
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.193"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(151..1425)
                     /gene="clpX"
                     /locus_tag="PN977_004679"
     CDS             complement(151..1425)
                     /gene="clpX"
                     /locus_tag="PN977_004679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001396736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATP-binding subunit ClpX"
                     /protein_id="HDI6044616.1"
                     /translation="MTDKRKDGSGKLLYCSFCGKSQHEVRKLIAGPSVYICDECVDLC
                     NDIIREEIKEVAPHRERSALPTPHEIRNHLDDYVIGQEQAKKVLAVAVYNHYKRLRNG
                     DTSNGVELGKSNILLIGPTGSGKTLLAETLARLLDVPFTMADATTLTEAGYVGEDVEN
                     IIQKLLQKCDYDVQKAQRGIVYIDEIDKISRKSDNPSITRDVSGEGVQQALLKLIEGT
                     VAAVPPQGGRKHPQQEFLQVDTSKILFICGGAFAGLDKVISHRVETGSGIGFGATVKA
                     KSDKASEGELLAQVEPEDLIKFGLIPEFIGRLPVVATLNELSEEALIQILKEPKNALT
                     KQYQALFNLEGVDLEFRDEALDAIAKKAMARKTGARGLRSIVEAALLDTMYDLPSMED
                     VEKVVIDESVIDGQSKPLLIYGKPEAQQASGE"
     gene            complement(1551..2174)
                     /gene="clpP"
                     /locus_tag="PN977_004680"
     CDS             complement(1551..2174)
                     /gene="clpP"
                     /locus_tag="PN977_004680"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006809880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HDI6044617.1"
                     /translation="MSYSGERDNFAPHMALVPMVIEQTSRGERSFDIYSRLLKERVIF
                     LTGQVEDHMANLIVAQMLFLEAENPEKDIYLYINSPGGVITAGMSIYDTMQFIKPDVS
                     TICMGQAASMGAFLLTAGAKGKRFCLPNSRVMIHQPLGGYQGQATDIEIHAREILKVK
                     GRMNELMALHTGQSLEQIERDTERDRFLSAPEAVEYGLVDSILTHRN"
     gene            complement(2420..3718)
                     /gene="tig"
                     /locus_tag="PN977_004681"
     CDS             complement(2420..3718)
                     /gene="tig"
                     /locus_tag="PN977_004681"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001716227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HDI6044618.1"
                     /translation="MQVSVETTQGLGRRVTITIAADSIETAVKSELVNVAKKVRIDGF
                     RKGKVPMNIVAQRYGASVRQDVLGDLMSRNFIDAIIKEKINPAGAPTYVPGEYKLGED
                     FTYSVEFEVYPEVELQGLEAIEVEKPIVEVTDADVDGMLDTLRKQQATWKEKDGAVEA
                     EDRVTIDFTGSVDGEEFEGGKASDFVLAMGQGRMIPGFEDGIKGHKAGEEFTIDVTFP
                     EEYHAENLKGKAAKFAINLKKVEERELPELTAEFIKRFGVEDGSVEGLRAEVRKNMER
                     ELKSAIRNRVKSQAIEGLVKANDIDVPAALIDSEIDVLRRQAAQRFGGNEKQALELPR
                     ELFEEQAKRRVVVGLLLGEVIRTNELKADEERVKGLIEEMASAYEDPKEVIEFYSKNK
                     ELMDNMRNVALEEQAVEAVLAKAKVTEKETTFNELMNQQA"
     gene            complement(4062..4379)
                     /gene="bolA"
                     /locus_tag="PN977_004682"
     CDS             complement(4062..4379)
                     /gene="bolA"
                     /locus_tag="PN977_004682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414969.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator BolA"
                     /protein_id="HDI6044619.1"
                     /translation="MMIRERIEEKLRAAFQPVFLEVVDESYRHNVPAGSESHFKVVLV
                     SDRFTGERFLNRHRMIYSTLAEELSTTVHALALHTYTIKEWEGLQDTVFASPPCRGAG
                     SIA"
     gene            4684..5262
                     /locus_tag="PN977_004683"
     CDS             4684..5262
                     /locus_tag="PN977_004683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HDI6044620.1"
                     /translation="MFKKILFPLVALFMLAGCAKPPTTIEVSPTITLPQQDPSLMGVT
                     VSINGADQRTDQALAKVTRDNQIVTLTASRDLRFLLQEVLEKQMTARGYMVGPNGPVN
                     LQIIVSQLYADVSQGNVRYNIATKADIAIIATAQNGNKMTKNYRASYNVEGAFQASNK
                     NIADAVNSVLTDTIADMSQDTSIHEFIKQNAR"
     gene            5306..6781
                     /gene="ampG"
                     /locus_tag="PN977_004684"
     CDS             5306..6781
                     /gene="ampG"
                     /locus_tag="PN977_004684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="muropeptide MFS transporter AmpG"
                     /protein_id="HDI6044621.1"
                     /translation="MSSQYLRIFQQPRSAILLILGFASGLPLALTSGTLQAWMTVENI
                     DLKTIGFFSLVGQAYVFKFLWSPLMDRYTPPFFGRRRGWLLATQILLLVAIAAMGFLE
                     PGTQLRWMAALAVVIAFCSASQDIVFDAWKTDVLPAEERGAGAAISVLGYRLGMLVSG
                     GLALWLADKWLGWQGMYWLMAALLIPCIIATLLAPEPTDTIPVPKTLEQAVVAPLRDF
                     FGRNNAWLILLLIVLYKLGDAFAMSLTTTFLIRGVGFDAGEVGVVNKTLGLLATIVGA
                     LYGGILMQRLSLFRALLIFGILQGASNAGYWLLSITDKHLYSMGAAVFFENLCGGMGT
                     SAFVALLMTLCNKSFSATQFALLSALSAVGRVYVGPVAGWFVEAHGWSTFYLFSVAAA
                     VPGLILLLICRQTLEYTRVNDNFISRTEYPAGYAFAMWTLAAGVSLLAVWLLLLTMDA
                     LDLTHFSFLPALLEVGVLVALSGVVLGGLLDYLALRKTHLT"
     gene            7241..8188
                     /gene="cyoA"
                     /locus_tag="PN977_004685"
     CDS             7241..8188
                     /gene="cyoA"
                     /locus_tag="PN977_004685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit II"
                     /protein_id="HDI6044622.1"
                     /translation="MRLRKYNKSLGWLSLFAGTVLLSGCNSALLDPKGQIGLEQRSLI
                     LTAFGLMLIVVIPAILMAVGFAWKYRASNKDAKYSPNWSHSNKVEAVVWTVPILIIIF
                     LAVLTWKTTHALEPSKPLAHDEKPITIEVVSMDWKWFFIYPEQGIATVNEIAFPANTP
                     VYFKVTSNSVMNSFFIPRLGSQIYAMAGMQTRLHLIANEPGTYDGISASYSGPGFSGM
                     KFKAIATPDRAAFDQWVAKAKQSPNTMSDMAAFEKLAAPSEYNQVEYFSNVKPDLFAD
                     VINKFMAHGKSMDMTQPEGEHSAHEGMEGMDMSHAESAH"
     gene            8210..>8440
                     /locus_tag="PN977_004686"
     CDS             8210..>8440
                     /locus_tag="PN977_004686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002444673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit I"
                     /protein_id="HDI6044623.1"
                     /translation="MFGKLSLDAVPFHEPIVMVTIAGIILGGLALVGLITYFGKWTYL
                     WKEWLTSVDHKRLGIMYIIVAIVMLLRGFADAI"
BASE COUNT         1947 a         2208 c         1994 g         2291 t
ORIGIN      
        1 gatgacacga ctgtgcttca cgccatctat taacatgtac gtcagtatat ggggatgttt
       61 cccccacatt caacgccgag aatagaggaa aaattaaagg ggagataaaa tccccccttt
      121 ttggttaact aattgtatgg gaatggttaa ttattcacca gatgcctgtt gcgcttccgg
      181 cttgccataa atcagcaacg gcttgctttg accatcaatt accgattcgt cgataaccac
      241 tttttcgacg tcttccatgg acggcagatc gtacatggta tcgagcagtg cggcttctac
      301 gatggaacgc aggccacggg caccggtttt acgcgccatc gctttcttag cgatagcatc
      361 cagcgcctcg tcacggaatt ccagatccac accttccaga ttaaacagcg cctgatactg
      421 cttggtcagg gcgtttttcg gctctttgag gatctgaatc agagcttctt cgctcagttc
      481 attcaacgtt gcgacaaccg gcagacgacc aatgaactca gggatcagac caaacttgat
      541 cagatcttcc ggttcaacct gcgccagcag ttcaccttcg cttgctttgt cggacttcgc
      601 ttttaccgtc gcgccaaaac caatgccgga accggtttct actcgatggg aaatcacttt
      661 atccagaccg gcaaacgcac cgccacagat aaacaggatc ttagaggtat caacctgcaa
      721 gaattcctgc tgcggatgtt tacgcccacc ttgcggcgga acagcagcta ccgtaccttc
      781 gatcagtttt aacagtgcct gctgtacgcc ttcaccggaa acgtctcggg taatggacgg
      841 gttgtctgac ttacgagaaa tcttgtcaat ttcatcgatg tagacaatcc cgcgctgcgc
      901 tttctgtacg tcgtaatcgc acttctgcaa cagcttctga atgatgtttt caacgtcttc
      961 gcccacataa ccggcttcgg tcagcgtggt tgcgtcagcc atggtgaacg gaacgtccag
     1021 caggcgcgcc agcgtttcgg ccagcagcgt tttaccggaa ccggtcggac cgatcagcag
     1081 aatgttactt ttgcccaact cgacgccatt gctggtatct ccgttgcgca gacgtttgta
     1141 gtggttgtat accgcgaccg ccagcacttt tttcgcctgt tcctgaccga taacgtaatc
     1201 gtccaggtgg ttgcgaattt catgcggcgt cggtagcgca ctgcgttcac gatgcggtgc
     1261 aacttcttta atctcttcgc gaatgatgtc gttacataaa tcaacacatt cgtcgcagat
     1321 atacacggat ggaccggcaa tcagcttgcg cacttcatgc tggcttttgc cgcaaaaaga
     1381 gcaatacagc aatttgcctg agccatcttt gcgtttatct gtcatgggtc aaaacctctt
     1441 ctttgttctt tgtgccgcac acgacgacgc aaatgccatt ctcaggcgca agccgcttac
     1501 agcgttgtgc cgccctggat aagtatagcg gcacagttgc gcctctggca tcaattacga
     1561 tgggtcagaa tcgaatcgac cagaccgtat tccaccgctt caggggcgga aaggaagcga
     1621 tcgcgctcgg tatcacgttc aatctgttct aatgattgac ccgtatgaag cgccataagt
     1681 tcattcatgc gccctttaac tttcagaatt tcacgggcat gaatttcgat atcggtcgcc
     1741 tggccctggt agccgcccaa cggctggtga atcatcacgc gcgaattcgg caggcagaaa
     1801 cgtttacctt ttgcccctgc agtcagcaag aaagcgccca tcgaggccgc ctggcccata
     1861 cagatggtgc tgacatcagg cttgataaac tgcatggtgt catagataga catcccggca
     1921 gtaatcaccc cgcctggaga gttaatgtac agatagatat ctttttctgg gttttctgct
     1981 tccaggaaca gcatctgcgc cacaatcagg ttagccatgt ggtcttcaac ctggccagtc
     2041 agaaaaatga cgcgttcctt aagtagacga gaatagatat caaaagagcg ctcacctcgt
     2101 gaggtctgtt caatgaccat cggcaccagc gccatatggg gtgcaaagtt atctcgttcg
     2161 ccgctgtatg acatttccgt ctcctggata aaattgaaaa aacctgctgt accgattgta
     2221 accttatgga cggattatca gctagtccct gtgttatggg tacggcatca ccatatttca
     2281 agcataacaa tcttttgttg ttacgctaac accgaaagag gcttttcgca cggttccatg
     2341 caaaattcat gacaaaaaaa agcccaccac ctggcggtga cgggcctttg tgcgaattta
     2401 gcgcgttatg ctgcgtaaat tacgcctgct ggttcatcag ctcgttgaaa gtggtttctt
     2461 tttcagtcac tttcgctttc gccagtacag cttcaacagc ctgttcttcc agagcaacat
     2521 tgcgcatgtt gtccatcagt tctttgtttt tgctgtagaa ctcgataact tctttcggat
     2581 cttcgtacgc agaagccatc tcttcgatca ggcctttcac gcgctcttcg tcagctttca
     2641 gctcgttggt gcggataact tcgcccagca gcaggccaac aactacgcga cgtttagcct
     2701 gttcttcgaa cagttcacgc ggcagttcca gagcttgttt ttcgttgcca ccgaaacgct
     2761 gtgcagcctg gcgacgcaga acgtcgattt cgctgtcgat cagcgcagcc ggtacgtcga
     2821 tgtcgttagc ttttaccaga ccttcgatcg cctgagactt aacgcggtta cggatggcgc
     2881 tcttcagctc gcgctccatg tttttacgca cttcagcgcg cagaccttct acggaaccat
     2941 cttcaacgcc gaaacgtttg atgaattctg cagtcagttc cggcagttca cgctcttcaa
     3001 ctttcttcag gttgatagcg aatttcgctg ctttaccttt caggttttct gcgtggtact
     3061 cttccgggaa ggtcacgtcg atggtgaact cttcgccagc tttgtggcct ttgataccgt
     3121 cttcaaagcc tgggatcata cgaccctggc ccatcgccag tacgaaatca gacgctttac
     3181 cgccttcgaa ctcttcaccg tctacagaac cggtgaagtc gatggttacg cggtcttctg
     3241 cttcaacagc gccgtctttt tctttccagg tcgcctgctg tttacgcaga gtatccagca
     3301 tgccgtcaac gtcagcgtcg gtcacttcaa cgatcggttt ttcaacttcg atcgcttcca
     3361 gaccctgcag ttcaacttcc ggataaactt caaactctac agagtaggtg aagtcttcac
     3421 ccagcttgta ttcgcccgga acataagtcg gtgcgccagc cggattgatt ttttctttaa
     3481 tgatggcgtc aatgaagtta cggctcatca ggtcacccag aacgtcctgg cgtacagacg
     3541 caccataacg ctgagcaacg atattcattg gcactttgcc tttgcggaag ccgtcaatac
     3601 gtactttttt cgcaacgttg accagctcgc ttttaacagc ggtctcgatg ctgtcagcag
     3661 cgatagtaat cgttacacgg cggccaaggc cttgagtggt ttcaactgaa acttgcatct
     3721 tgttacctca aaaaatcaca gtgctcggtc aactcttacc ccgcaagcac aggttgttgg
     3781 cttcgcggaa ccgggatgtt cttataatca atcacattcc ctgttaccag aatcatcccg
     3841 aaagcattca aataggacgc ggcattatag cggcatcact tttatgagtc gagaacggtt
     3901 attgcgcgct gtcgcgtatt ttttcacaat tcccgggcaa ttttcgtctg aatgctgggc
     3961 aaaaagcgca aaatccagac aaaacaaaac ggcccgcagg ccgtttttca taaaatcttt
     4021 agcaacatac tggaaaagcg ccgacagttg caaatgcgtt tttacgcgat gcttcctgct
     4081 ccacgacagg gaggagaggc aaagacggtg tcctgcaacc cttcccactc cttaatagtg
     4141 taagtatgca gagccagcgc atgaacggta gtagagagtt cctccgctaa agtactgtaa
     4201 atcattcgat gacgattcag aaaacgttca cccgtaaaac gatcgctgac cagcacaact
     4261 ttaaaatggc tttcagagcc ggctgggaca ttgtgacgat agctttcatc cactacttcg
     4321 aggaatacgg gttggaacgc cgcccttaat ttttcttcta tccgctcacg tatcatcatg
     4381 aaatatctcc tccgacaacg ctgagatgtc atccatccct ttaaatacta gccgctttta
     4441 ccgtttccat tgcagcttaa caacaaatat ttaggtttcg tcctggtttt tccgtcaagc
     4501 gtatgaagtt tactgattta aagattgaaa cacaggcctc gcatcagcgt ttcttttacc
     4561 aggggcgatt atttcgtcaa catgatgaat ttacatgcct tacccacttc ccctcgccgt
     4621 tggcaatgtt atgatggcgg gaatttttac caaacacgct taagagtctc tgagagcccg
     4681 aacatgttca aaaaaatcct cttcccgtta gttgctctgt ttatgcttgc aggatgcgca
     4741 aaaccgccaa caactattga agtttcaccg acgataacac tgccacagca ggatccaagc
     4801 ctgatgggcg tcaccgtaag cattaatggt gccgatcagc gtaccgatca ggcgctggca
     4861 aaagtcaccc gcgataatca aatcgttacc ctgaccgcct cccgcgatct gcgtttcctg
     4921 ctgcaagaag tgctggaaaa acagatgacc gcgcgtggtt acatggttgg tccgaatggc
     4981 ccggttaatc tgcaaatcat cgttagccaa ctgtatgctg acgtgtccca gggcaacgtg
     5041 cgctataaca tcgccaccaa agcggatatc gctatcatcg ctaccgcgca gaatggcaac
     5101 aaaatgacca aaaactatcg tgccagctac aacgttgaag gtgcgttcca ggcctccaac
     5161 aaaaatatcg ccgatgcggt taacagtgtg ctgaccgata ccatcgctga tatgtctcag
     5221 gacaccagca tccacgaatt catcaagcag aacgcgcgtt aatttctgcc ctctggcccg
     5281 gtgcaagtcg ggcctgtaga cgcccatgtc cagtcaatat ttacgtattt ttcaacagcc
     5341 gcgttcagcc atattgctga ttctgggttt tgcttccggg ctaccgctcg ccctgacatc
     5401 cggcacctta caggcctgga tgacggtcga gaatatcgat cttaaaacca ttggtttctt
     5461 ctctctggta ggccaggctt acgtttttaa attcctctgg tcaccactga tggaccgcta
     5521 cacgccccca tttttcgggc ggcggcgcgg ttggctgctc gccacgcaaa ttctgttatt
     5581 agtcgccatt gcggcgatgg gttttctcga accaggcacc cagctccgct ggatggcggc
     5641 actggcggtg gtgatcgctt tttgctctgc ctcccaggat attgtcttcg atgcgtggaa
     5701 aaccgatgtg ctcccggcag aagaacgtgg tgcgggcgcg gcaatcagcg tgctgggtta
     5761 ccgtttaggg atgctggttt ccggcggctt agccctgtgg ctggcagata aatggctggg
     5821 ctggcagggc atgtactggt tgatggcggc gctgttgatc ccctgtatta tcgcgacgtt
     5881 gcttgcacca gaacccaccg acaccattcc tgtgcccaaa acgctggaac aagcggttgt
     5941 tgcacctctg cgcgatttct ttggtcgcaa taatgcctgg cttattttgc ttcttatcgt
     6001 gctgtataag ctgggcgacg cattcgctat gagcctgaca accacgtttt tgattcgcgg
     6061 cgtcgggttt gatgcgggtg aagtaggcgt ggttaacaaa acgcttggct tactggcgac
     6121 cattgttggc gcattgtacg gtgggatttt gatgcagcgc ctgtcgctgt tccgtgcact
     6181 gctgattttc ggcattttac aaggtgcatc taacgccggt tactggctgc tgtcgattac
     6241 tgataagcat ctctacagca tgggtgcagc cgtctttttc gaaaacctct gtggcgggat
     6301 gggtacatca gcctttgtcg cgctgttaat gacgctgtgt aataagtcat tttccgctac
     6361 tcaatttgcc ctgctctcag cgctttctgc tgtagggcga gtttatgtcg gccccgtggc
     6421 gggttggttt gttgaagcac acggctggtc gacattctat ctgttctccg tcgccgctgc
     6481 cgtaccaggg cttattttac tgctgatttg tcgccagacg cttgaatata cacgagtaaa
     6541 tgacaacttt atctcccgta ccgaatatcc ggcaggttat gcctttgcca tgtggacact
     6601 ggcggcgggc gtcagcctgt tggccgtgtg gttactgctg ttgacgatgg acgcgctgga
     6661 tttgacgcac ttctctttcc tgcctgctct gctggaagtc ggggttttag tcgccctttc
     6721 tggcgtcgtg cttggtggtt tactggatta tctggcgcta cgaaaaacgc atctgacgta
     6781 atctgtaaat attatttagc tgtagtaatc actcgccgta attattacgg ctaaataaac
     6841 atcagcatcg cttattaata tttgttcttt tgtgcggctt agcgtctgga aattattgac
     6901 gctatttatg aattctattt ttcttacacg attcagcaaa tgagtcttta ttttctcact
     6961 accaagttgt caccttaatg ataattattt gttaaataat tgttttattt cgcattggtt
     7021 ataccaattg cccgcccaga ctccttctgc actccccttt tgttataacg cccttttgca
     7081 acagcttctt aaaatcaatc cgatatgttt tgcaacatat gtgacctgga agccaaatcc
     7141 aagtaacagg aatttaatca tgtttacagt aatgtaacct tcccgtaaaa tgcccacaca
     7201 ctttaaacgc caccagatcc cgtggaattg aggtcgttaa atgagactca ggaaatacaa
     7261 taaaagtttg ggatggttgt cattatttgc aggcactgta ttgctcagtg gctgtaattc
     7321 tgcgctgtta gatcccaaag gacagattgg tctggagcaa cgttcactga tactgacggc
     7381 atttggcctg atgttgattg tcgttattcc cgcaatcttg atggctgttg gtttcgcctg
     7441 gaagtaccgt gcgagcaata aagatgctaa gtacagcccg aactggtcac actccaataa
     7501 agtggaagct gtggtctgga cggtacctat cttaatcatc atcttccttg cggtactgac
     7561 ctggaaaacc actcacgctc ttgagcctag caagccgctg gcacacgacg agaagcccat
     7621 taccatcgaa gtggtttcca tggactggaa atggttcttc atctacccgg aacagggcat
     7681 tgctaccgtg aatgaaatcg ctttcccggc gaacactccg gtgtacttca aagtgacctc
     7741 caactccgtg atgaactcct tcttcattcc gcgtctgggt agccagattt atgccatggc
     7801 cggtatgcag actcgcctgc atctgatcgc caacgaaccc ggtacttatg acggtatctc
     7861 cgccagctac agcggcccgg gcttctcagg catgaagttc aaagctattg caacaccgga
     7921 tcgcgccgca ttcgaccaat gggtcgcaaa agcgaaacag tcgccgaaca ccatgtctga
     7981 catggcagcg ttcgaaaaac tggccgcgcc tagcgaatac aaccaggtgg aatatttctc
     8041 caacgtgaaa ccagacttgt ttgctgatgt gattaacaag tttatggctc acggtaagag
     8101 catggacatg acccagccag aaggtgagca cagtgcacac gaaggtatgg aaggcatgga
     8161 catgagccac gcggaatccg cccattaaag gggttgagga agaataaaga tgttcggaaa
     8221 attatcactt gatgcagtcc cgttccatga acctatcgtc atggttacga tcgctggcat
     8281 tattttggga ggtctggcgc tcgttggcct gatcacttac ttcggtaagt ggacctacct
     8341 gtggaaagag tggctgacct ccgtcgacca taaacgccta ggtatcatgt atatcatcgt
     8401 ggcgattgtg atgttgctgc gtggttttgc tgacgctatt
//
LOCUS       DAMZFT010000194         7905 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.194, whole genome shotgun sequence.
ACCESSION   DAMZFT010000194 DAMZFT010000000
VERSION     DAMZFT010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7905)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7905)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7905
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.194"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(<1..125)
                     /locus_tag="PN977_004687"
     CDS             complement(<1..125)
                     /locus_tag="PN977_004687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000086499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HDI6044624.1"
                     /translation="MSRIESDFLGEREVPDDCYYGVQTLRGEDNFHITEMPMSQEP"
     gene            991..2247
                     /gene="pepT"
                     /locus_tag="PN977_004688"
     CDS             991..2247
                     /gene="pepT"
                     /locus_tag="PN977_004688"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001595907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HDI6044625.1"
                     /translation="MDIVERFINYTKINTTTSRENGAKGIMPSSPGQMKLAKLLVSEL
                     EALGMEDIILRENAIVTATLPANTDETIPVVSFFGHLDTSAEQTADTKAQRLPYNGGD
                     LCLNPELNIYLRESEFPELKNYIGDDLIVTDGTSLLGADDKAALAAIMNALQFLISHP
                     EIRHGEVKVGFVPDEEQGLRGAKAFDVSEFGADFGYTLDCCGIGEFVYENWNAGDAEI
                     IFTGQSAHPMSAKGKLKNSLLMAHKFISMLPGGEAPEYTEGREGYYWVKQLQGNSART
                     VLKLDIRDFSEEGYHARKTFVRQLAESACALWGEGSVICQLSDRYANVFNSLQGEGHY
                     PIDIALRAYQRCGITPTPVAMRGGYDGAVLSQKGLPCPNIFTGAHNFHSIYEYLPVRS
                     LRAASDVVIAIIQETFNGFTTGNRES"
     gene            2244..3605
                     /gene="dcuC"
                     /locus_tag="PN977_004689"
     CDS             2244..3605
                     /gene="dcuC"
                     /locus_tag="PN977_004689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003021260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate transporter DcuC"
                     /protein_id="HDI6044626.1"
                     /translation="MIKVVIALLVIVIVARLILKGYRAEPVLFIAGLALMVCTWFTGW
                     GTVLPKGVSGTGISFLDPFEVMRNLFSTRAADLGLMIMTLMGFAHYMDHIGANEAVVR
                     VVTRPLRTLRSPYVLLFFSYLFASLLQLAIPSATGLAVLLMGTMFPIMLGLGLSAASA
                     AGVIATSLGVAYTPTAIDAIRGSEAVNMDVVEYVVYHQGPAALATVLIVGISHFFWQK
                     HCDRKAGTLPHEIGTTTVIKAGSTPAYYALLPMLPILMAVGSSEIFVTGINLNIITIV
                     LISMAICMLIEWVRKCDLKAVCDGFTHFLKGMGTAFTGVVGLLVAAGVFAHGIKSIGA
                     IDQLILMAEHVGLPPFAMGIVFALVTLAAAVIMGSGNAPFLAFVELIPQIAASMGVNA
                     ISMILPMQQASHMGRAMSPVSGVVIAVSSGANITPFEVVKRTALPLIVGFVFHSAIIG
                     IFY"
     gene            complement(3658..3885)
                     /locus_tag="PN977_004690"
     CDS             complement(3658..3885)
                     /locus_tag="PN977_004690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001333103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence protein"
                     /protein_id="HDI6044627.1"
                     /translation="MEDFHLLVVLQPPQLKNSASHGATRHARRTYAASADVNGTRNIL
                     AAGHAVLACKEMVQSGLLFKQEPTEMIPAIA"
     gene            4251..4697
                     /locus_tag="PN977_004691"
     CDS             4251..4697
                     /locus_tag="PN977_004691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001333102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maturase"
                     /protein_id="HDI6044628.1"
                     /translation="MMINEAHAQATAASGRGDGQYPSGLHDGAEISTAAGGQTKAEVP
                     LTMEAVITRENLMLAYQRVVENKGVSELKPWLKKNWRSVWQVLIDGNYQPRAIRRMDI
                     PKPDGGVRTLGIPTVVDRLIQQAIAQQLSAIVDTASAATQTTATST"
     gene            4745..4894
                     /locus_tag="PN977_004692"
     CDS             4745..4894
                     /locus_tag="PN977_004692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001327936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reverse transcriptase"
                     /protein_id="HDI6044629.1"
                     /translation="MESKLKQKVNEKKSAVARPWERKLWADSDGPCCFTKRLGRQAEL
                     GSLRT"
     gene            5221..6252
                     /locus_tag="PN977_004693"
     CDS             5221..6252
                     /locus_tag="PN977_004693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoaspartyl peptidase/L-asparaginase"
                     /protein_id="HDI6044630.1"
                     /translation="MNKIKGITVMAAFALCIGYAHAESQTPIRLVIHGGAGTITKDTI
                     TPEQEKQYREKLTDALNAGYAVLNSGGSSLDAVQRAINVMEDSPLFNAGKGAVFTHDG
                     KNELDAAIMDGKTKMAGAVAGVTTIKNPINAAYAVMTKSPHVLMISNGAELFAKEQGL
                     VMVEPAYFKTDFRWQQLQNALKDEKITLDHNGKSASLLLPPKNYDYKYGTVGAVALDK
                     DGNLAAGTSTGGMTNKRYGRVGDSPIIGAGTYADNNTVAVSATGTGEMFIRTSTAYNI
                     AAQVKYKNTPLKEAAQNALDEVKNINGSGGVIVLDKNGNYTMSFNTEGMYRGTIGNDS
                     KAIVSIYEQ"
     gene            complement(6590..6868)
                     /locus_tag="PN977_004694"
     CDS             complement(6590..6868)
                     /locus_tag="PN977_004694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000849708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5339 domain-containing protein"
                     /protein_id="HDI6044631.1"
                     /translation="MKTITVLLTTVLFSMSASAALTPACEEYYKEIDNFVAKMKEMGT
                     PEAQVNTLKQQYEQSRKQIAALPDASQDMACKQGLDALRQSMSAAGIK"
     gene            7314..7905
                     /locus_tag="PN977_004695"
                     /pseudo
     CDS             7314..7905
                     /locus_tag="PN977_004695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015869530.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         2289 a         1645 c         1848 g         2123 t
ORIGIN      
        1 ggctcctgac tcattggcat ttcagtaata tggaaattat cctcaccacg aagtgtctga
       61 acaccataat agcaatcatc cggaacttca cgctcaccaa ggaaatcgct ttcaatacga
      121 gacataattt tctaactcca tttttcgaaa acaatgtgac aataacaaat tgttttcata
      181 aaacacttat ccaacattaa gaatgaatac ttataatcac cagatgacat aaccattacc
      241 atctaatgga atatatttaa actaataaga taaacttaaa acgtgatatc aagcacattt
      301 ttataacgtt aaaattagtg ggttatttag caatcaaata taaccagtaa aagcccgaaa
      361 tatgcagata acgctgcact aatatgatta agatcaaaaa aaacaattcc gcaatcaaac
      421 ttattattaa tgctgatttt ttaaatcatg attaagtaga actattttat aatagcatcc
      481 cggtttggtc acatccttca gaaaaacaac ctgtttacca ccatagaaaa tctatatctc
      541 tgctaggttt tccagcgttg tagatatagt cactcatggt gattcttatt aagataagag
      601 tttttacacg ggaatgttat tgcacttcta attgataaat cttggaatag agtagaaaac
      661 gtaatgtttc cactccatct tccgctttaa tggtttgtaa tttgagttag atcatggatt
      721 ttaacatcca ttatgattaa ttatttttag atgatttgat cactaaatcg tctttcaggg
      781 tgtaacggca ttaatcatca caagctaaaa aaacgccata ctgtaatgga gtctgtattt
      841 tatgttcaat catgcaaagg ctcttcataa ttatcatttc tctcttggaa aaatgacagc
      901 ctgaacatag cataaattca cgttcttcag agtcagaagg tgttaataaa ctgtccctaa
      961 aatctggaaa atgttgaaga aggcatctca atggatattg tcgaacgctt tatcaattat
     1021 acaaaaatta ataccactac ctcacgagaa aacggcgcaa aagggatcat gccttcatct
     1081 cctggtcaga tgaaacttgc gaaattgttg gtgagtgaac ttgaagcatt gggtatggag
     1141 gatattattc tcagagagaa tgctatcgta acagcaacac tccctgcaaa tactgacgaa
     1201 acaatccccg tcgtttcctt ttttggacat ctggacacca gtgccgaaca gacagccgat
     1261 acaaaagcgc aacgattacc ttataatggg ggcgatctct gtctgaaccc tgaactgaat
     1321 atttatttgc gtgagagtga attcccggag ctcaaaaatt atattggcga tgaccttata
     1381 gtgacagatg gtaccagcct tctgggggcc gatgataaag cggcgctggc ggccataatg
     1441 aacgcacttc aatttctgat ttctcatcct gaaatcaggc atggagaagt taaagttggg
     1501 tttgtgccag atgaagagca ggggttacgg ggggccaaag ccttcgatgt ttcagagttt
     1561 ggcgcagatt ttggctacac tctggattgc tgcggtattg gagaatttgt ttacgaaaac
     1621 tggaatgcgg gtgatgcaga aattatcttt accggccagt ctgcacaccc tatgtcagcg
     1681 aaaggcaagc ttaagaactc tcttttgatg gctcacaaat tcatttcgat gttgccagga
     1741 ggagaagcgc ctgaatatac agaaggacgc gaaggctatt attgggtgaa acagttacag
     1801 ggaaacagtg ccagaactgt actaaaactg gatatacgag atttcagcga ggaaggatat
     1861 cacgcccgga agacatttgt acgccagctt gcagaaagtg cttgtgcatt atggggagaa
     1921 ggaagcgtga tctgccaact gagcgatcgg tacgccaatg tctttaacag tctgcaggga
     1981 gaggggcatt atcccatcga cattgccctg cgagcttacc aacgatgtgg tattactccg
     2041 acaccggtgg cgatgcgtgg aggatatgat ggcgctgttc tttcacaaaa aggattaccc
     2101 tgcccgaaca tttttaccgg cgcacataat ttccattcta tctatgaata tcttccagtc
     2161 cgttcacttc gggcggcaag tgatgtggtt attgccatca ttcaggagac attcaatggg
     2221 ttcaccacag ggaatcgtga gtcatgatta aagttgttat cgcgcttctt gttatcgtga
     2281 tagttgctcg cttaatcctg aaaggatatc gtgcagaacc cgtgcttttt attgccgggc
     2341 ttgcgctgat ggtttgtacc tggtttaccg gttggggcac agtgttaccg aagggcgtgt
     2401 ccggtacagg aattagtttt cttgatcctt tcgaagtcat gcgcaattta ttcagtaccc
     2461 gtgcggctga tctcggtctg atgattatga ccctgatggg atttgcgcat tacatggacc
     2521 atatcggtgc gaatgaagcg gttgtccgtg tggtaacccg gccattacgg acattacgtt
     2581 ccccctatgt tctgcttttt ttctcttatc ttttcgccag tcttcttcaa ctggctatcc
     2641 cctcagctac cggacttgct gttttactga tgggaaccat gtttcctatt atgctcggac
     2701 tcgggctgtc agcagcgtct gccgcagggg taattgccac ctctcttggt gtggcgtaca
     2761 caccgacagc cattgacgcc atacgaggct ctgaagcggt aaatatggat gtggtggagt
     2821 atgttgtcta tcatcagggg cctgctgctc tggcgactgt tctgattgtc ggtatcagcc
     2881 actttttctg gcagaaacac tgtgatcgta aagctggaac attacctcat gaaattggta
     2941 cgacaacggt cataaaagca ggcagtacgc cggcttatta tgctctgcta cctatgctac
     3001 cgatcctgat ggccgttggc tcatcggaga tttttgtcac cggaattaat ctcaatatca
     3061 ttacgattgt tcttatctca atggcaattt gcatgctgat cgaatgggtc cgtaaatgtg
     3121 atctgaaagc tgtatgtgac gggtttaccc atttcctgaa aggaatgggg acagcattta
     3181 ccggcgtggt gggcttactg gtggccgcag gtgtgtttgc tcatgggatt aaaagcattg
     3241 gcgcgataga tcaattaatt ttgatggcag agcacgttgg gttaccccct tttgcgatgg
     3301 gcatcgtttt tgctctggtt acactggctg cagctgtcat tatgggatcg ggcaatgcgc
     3361 catttctggc ttttgttgaa ctgataccac aaatcgctgc cagcatgggc gtgaatgcca
     3421 tatcgatgat cctgcctatg cagcaggctt cccatatggg gcgtgcgatg tctcccgtat
     3481 ctggcgtggt tatagcggtt tccagtggag caaatataac tcccttcgaa gtggtaaaac
     3541 gcactgcttt acccctgata gttggttttg tttttcactc tgcgattatc ggtatttttt
     3601 attaacttgt tttagattgt catcctccac gcccagaagg atgtggattc ctgctaatca
     3661 ggctattgcc ggaatcattt cggtgggctc ctgcttaaac aaaaggcctg actgcaccat
     3721 ctctttacag gcaagtacgg cgtgccctgc agctaaaata ttacgggtgc cgtttacatc
     3781 ggcgctcgct gcatatgtgc gccgggcatg gcgcgtagcg ccgtgactgg cgctgttctt
     3841 caactgtggt ggctgaagaa cgactaagag gtgaaagtcc tccacacacc cggtgagggg
     3901 aagtgttagc ggaaggcaag ggtgttcatc gcgaggtgaa atctgaagga agccggatgc
     3961 aaaatgctgg cctgacgaac aggaagcggt taaggcggcg cagcagggta aggtggcaca
     4021 aatcaccaaa gcccgatact cacacggaac gctgtgacgt aaagccgaca ggcataagca
     4081 ggaaagtcgc gtgaattacc ccgggagatc tgtcgccctg ccagtgtgct accggtgccg
     4141 tgaggcgccg ggatggggcg gcagaagtca gccgacgccg tagtagtgct gcaaaaccgg
     4201 cagcatgaag ggccgaacat gattaactgt gattaggaca tccgaacccg atgatgatta
     4261 atgaagcaca cgcgcaggca actgcggcca gcggcagagg agacggacag tatccgtcag
     4321 ggctgcatga tggcgctgaa atcagcacgg cggcgggtgg gcaaacgaaa gcggaagtgc
     4381 cgctgacgat ggaagcggtg ataacgagag aaaacctgat gctggcttat cagcgcgtgg
     4441 tggaaaacaa aggtgtgtcg gagttgaaac cgtggctgaa gaagaactgg cgcagcgtat
     4501 ggcaggtatt gattgacgga aactaccagc cgcgggcaat acgccggatg gatatcccga
     4561 agccggatgg cggcgtgaga accttaggca tcccgacggt ggtggacagg cttatccagc
     4621 aggcgatagc gcagcagtta agcgccatcg tggacacagc ttctgccgct acgcagacga
     4681 ctgcaacatc tacgtaagca gccggaaagc gggcgaccat ctgctgaagg acatcagggc
     4741 gttcgtggag agcaaactga aacagaaggt gaatgagaag aagagcgcgg tggcgcgccc
     4801 gtgggagcgg aagttgtggg ctgacagtga cggaccgtgc tgcttcacca aacgccttgg
     4861 acggcaggcg gaacttggat cgttacgtac ttgaaaagtg gatcaacaag agggttcaac
     4921 gagactgggg agttatattt ctttctcaag gtccggcatt tatctcttcc acatgaaatc
     4981 tcgatataaa gtcagacacg gatgtcctac tgaaagcggg gggaagtatg gtgatagagg
     5041 tcagcgggaa cctatccata aaagtcactc attacactaa aaaatatgtg catattattt
     5101 gcgtgcaatt aaattttgca aaatcgaatt agcataattt tttttgatag aagtcaaata
     5161 aacataatgg aaaaagttta aatatttata ttgtataaac ttcagacaaa aggaggagtt
     5221 atgaataaaa taaaaggtat aacagtaatg gcagccttcg cattgtgtat aggatatgct
     5281 cacgctgaat ctcaaacgcc aatacgcctt gtaattcatg gtggagctgg caccataaca
     5341 aaagatacta ttacacctga acaagaaaaa caatacagag aaaagttaac agatgcacta
     5401 aatgctgggt atgctgtgct taatagtgga ggaagtagcc ttgatgctgt tcagcgtgca
     5461 attaatgtta tggaggactc tcccctcttc aatgctggaa aaggcgctgt ctttactcat
     5521 gatggcaaaa acgaacttga tgctgcaatt atggatggaa aaaccaaaat ggccggagcc
     5581 gttgcaggag tgacgaccat aaaaaaccct ataaacgctg cttatgcagt tatgactaaa
     5641 agccctcatg tattaatgat ctctaatggt gcggaactat ttgcaaaaga gcaaggctta
     5701 gttatggttg agccagcata tttcaaaact gatttccgtt ggcaacagct acagaacgct
     5761 ttaaaagacg aaaaaataac ccttgatcat aatggtaaat cagcatctct tttactcccc
     5821 ccaaagaact acgactataa atatggaact gttggagcag tcgcgttgga caaagatggt
     5881 aatctggcag ctggaacatc aacaggaggt atgacaaata aacgttacgg acgcgtaggt
     5941 gactcgccaa taattggagc aggaacctat gccgataata ataccgttgc tgtttctgct
     6001 acaggtactg gagaaatgtt tattagaact tcaactgcat acaatattgc tgctcaggtg
     6061 aaatataaaa atacaccatt gaaagaagca gctcaaaatg cgcttgatga agttaaaaac
     6121 attaatggta gtggtggtgt cattgttcta gataagaatg gtaattatac tatgagtttc
     6181 aatacagaag ggatgtatcg tggaactatc ggcaacgata gtaaagctat cgtatcaatt
     6241 tatgaacaat agataaaata ttttatacat cgtcttgccc ttaaggccca ttttaaaagc
     6301 gggcctttat ttaataaata ttcttaacgc ctccttattt cacagctaaa agaagagtaa
     6361 ttcttagact cattaaccgt ctctctaccg agacgcttac ccatatccag cccgtaccct
     6421 gcagaaatac cgcgatgtat agcgtttctg tgtcatggtg aaaagtacag gaggaaatta
     6481 taaattctgg gaatatccct ggttataaca ttttgtaata cattaagtga tatcattatt
     6541 tatgatgtaa aaaggggcct gcaatagccc ctgaaaaatc agaaaacctt cattttatac
     6601 ctgcagcgct cattgactgc cgcagtgcat ccagtccttg cttacaggcc atatcctgac
     6661 tggcgtcagg taaagctgca atttgttttc gggattgttc atattgctgt ttcagcgtat
     6721 taacctgtgc ttctggtgtt cccatttcct tcatctttgc tacaaaatta tcgatttctt
     6781 tataatactc ttcacatgcc ggagtcagtg ctgcactggc tgacatactg aataatactg
     6841 tagtaagtaa gactgtaatt gttttcataa atacactcct tttttgtaca aaaacactca
     6901 accttcctga gaaggccata aaagcagaat gttattggaa tatcagcaaa acgagtattc
     6961 ccacaggttt tttcttggta tcagaattct gggatataaa tccagtaaat gatggagtaa
     7021 gtccgaaaga atatgtggtg tgattacgga ctaccagtaa atattactga tttcatgcca
     7081 tgaaaatact cattataaaa agcgcctgta tgctgcttat aaaatatcag aatccgatgt
     7141 gtcaaaatgt gatatatatt aaaaaataaa taagactgac ccaatatacc aaaagaggat
     7201 gacagaagtg aaggataacc gtattattct ggaccagaaa ggagcctgta catagatttg
     7261 tgtaattgcc tgattttgat atgttcaatt caccttcaaa tgaaggtgaa tttatggccg
     7321 acaaacaatt gcaggctctg gctaacgaac tggccaaaaa tctcaaaact cctgaagatc
     7381 tcagtcagtt cgatcgcctg ctcaagaaaa tcaaagttga ggctgctctc aacgctgaac
     7441 tgactcacca tctgggacat gaaaaaaatc aacccaaatc gggaaccaac tcccgtaatg
     7501 gctattccac gaaaaccgtc ccactaccga cgggccgatg gaattacgta ctccacgtga
     7561 tcgtgatggt tccttcgaac cacaactggt gaagaaaaat aagacctgca ttatcggcat
     7621 gaacaaccag atcctggcgc tgtatgccag agggatgact actcgcgaaa ttacctcagt
     7681 cttcaaagaa atgtacgatg cagacgtttc tccggcgctg atatcgaaaa tcaccgatgc
     7741 cgtaatcgac caggttgtag aatggcaaaa ccgtcctctg gatgcgattt atcccattgt
     7801 ttatcttgac tgtattgtcc tgaaagtacg gcaggacagc cgggttatca gcaaagcggt
     7861 gttcctggct ctgggtatta accttgatgg tcagaaagag ctgcc
//
LOCUS       DAMZFT010000195         7886 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.195, whole genome shotgun sequence.
ACCESSION   DAMZFT010000195 DAMZFT010000000
VERSION     DAMZFT010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7886)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7886)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7886
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.195"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(563..1120)
                     /locus_tag="PN977_004696"
     CDS             complement(563..1120)
                     /locus_tag="PN977_004696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001677489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HDI6044632.1"
                     /translation="MKGKTAAGGGAICAIAVMITIVMGNGNVRTNQAGLELIGNAEGC
                     RRDPYMCPAGVWTDGIGNTHGVTPGVRKTDQQIAADWEKNILIAERCINQHFRGKDMP
                     DNAFSAMTSAAFNMGCNSLRTYYSKARGMRVETSIHKWAQKGEWVNMCNHLPDFVNSN
                     GVPLRGLKIRREKERQLCLTGLVNE"
     gene            1290..1778
                     /locus_tag="PN977_004697"
     CDS             1290..1778
                     /locus_tag="PN977_004697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000068859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HDI6044633.1"
                     /translation="MAQRGVNKVILIGTLGQDPEIRYIPNGGAVGRLSIATNESWRDK
                     QTGQQKEQTEWHKVVLFGKLAEIASEYLRKGSQVYIEGKLKTRKWTDDAGVERYTTEI
                     IVSQGGTMQMIGARRDDSQSSNGWGQSNQPQNHQQYSGGGKPQSNANNEPPMDFDDDI
                     PF"
     gene            1976..2764
                     /locus_tag="PN977_004698"
     CDS             1976..2764
                     /locus_tag="PN977_004698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000126782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6044634.1"
                     /translation="MTAQNTKTIQYRLRNGQSVEVTINNDGVPGEKVSISDLAIEKTI
                     MCHLGFTEEVSKKHGVAIWSAMDTGMRKFITARTPGMTMMDLMQIAPLFECEPLDVFS
                     NPAICQQLYGEMKLAVTPIVLHEGSLAGVWKVERISSYMPFHVNGVITGENQPVSVIK
                     SDLKRAILEASCRVVGLGKQSYVSFPAGPEGPAEILIMDADLLWQIQFMIGKSIIRAE
                     ELDQYITCTMTDEVKSVATTNARSLFRAALAEPQENIPEETESD"
     gene            2962..4266
                     /gene="yjjJ"
                     /locus_tag="PN977_004699"
     CDS             2962..4266
                     /gene="yjjJ"
                     /locus_tag="PN977_004699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013359111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HipA family toxin
                     YjjJ"
                     /protein_id="HDI6044635.1"
                     /translation="MATHATTIRNLLNQGPMTPRQLVERLSVSQPTISRALRDLGDEI
                     VRIGNGRSIQYALRDSFRGFESVPIYRITEEGCICVLGTLIPVRPEGYVMVEEGKNNR
                     HSDGLPWWLFDMRPQGFLGRAYASAYALELNLPSNPEQWSDTDVIKALLKHGHDVVGN
                     LLIGEQARTHFLEMQPPVPVNRESEYPVLALAAGEGDVPGSSAGGEQPKFCTFTERGH
                     VLVKFSAPNDTPISERWRDLLFAEHLALKVLGVETEVFDYNGQRFLEIPRFDRVGQLG
                     RVGVFSLRALDAEFVGNARATWPLLVKELVKNKCVHPDAIIIATRLWSFGLLIGNTDM
                     HNGNLSFISSHGRPYHIAPAYDMLPMGFAPRSGGAISNELRKTALPDFVSRKIWDESL
                     ELAEKFYALACESDKFSANFTPCLEALRIHLDDCKIRIARLA"
     gene            complement(4298..4606)
                     /locus_tag="PN977_004700"
     CDS             complement(4298..4606)
                     /locus_tag="PN977_004700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6044636.1"
                     /translation="MQIKIAAPLGGDAIIEFDDNEEVSGRLSIISGDITEDMIAEAIA
                     GANPNSYMGFVNTLDAPASDVLRTLHLYAGWFVDWPAVDGGDEDDDDDFGDHVNQIVY
                     "
     gene            complement(4596..7583)
                     /locus_tag="PN977_004701"
     CDS             complement(4596..7583)
                     /locus_tag="PN977_004701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000058793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6044637.1"
                     /translation="MSLSDQVVMATSIETLIELLKNLPDYGRVSYVVTAKGDEVKTAF
                     DIVDASALLVSNTLDGKINPDYPQELQPRDRTRASSLLQVNQISKDLRPAQLTDSGLS
                     SHGAPIIGEDNAVESGNGRTMGIIKAYQDGNADRYREYLIEHATEFGIRPEKVESMTA
                     PVLVRRRLTKVDRVQFAKDSNISDLQEMAASEKAFVDADSITPAMMALFNPSESGDLL
                     SRSNDAFIRGFMTQVGATQAAGLVTEDGRPTRQLVDRIQNAIFAKAYKDARLVRMVAE
                     EPDPDMRNVLTALNAAANDFVQMQALSGEAHKQAVTTIVDGIETADSLDKKALAALKD
                     AVDLVRQSKESGQHITDVIAQGDMFSEMAPEVKALALFIVANNRSAKRMATAFKLMAQ
                     RINDELQHQGQALGDMFGGGDVSLQDILRQVSQELENEGMQGISGGLFESVSGGSYNG
                     VAPYTSLLLHRTSGIKDIIHLIRLLSRTDPQDEQLVQVLAHFVRMPVADVKKWCRLFG
                     ISNSLLRGLLNHASSLGRDGFDEIAQAIKNGDMPPAIDWFSIRPTRVKAFLSAAHSAS
                     PLAEMVQRLSLIFTDHTALGDLTLDEMKEASIQWADQQNEVNSDFLPAFRKAVSKADD
                     ARGILKAFKALQSRVNKHVGDIDGVTAEGRDILKEHGITPEFIDEIRTDMQREVVSSL
                     QIVARALADANPKSAAIVNRVIGDIEASEGMGALKLFLSRAFNPNGNILPGIIGEAKK
                     YVSEEELEQLDQLLKRFSYNPQTRWQMNQRSMGSVHEKVLSAMNSAIANSSVSEEKAL
                     EWADSFITEEVEEARAGQNGGIDLRKELADIYRLTGGKISTLSKVVHHQGRAYANLNG
                     VVAVNLNDENASALWHELGHHLEYSNPGLLEKARSFLKANVEGDKPSFVNIGGRGKPE
                     WCFRSRLSNIYMAKVYPPASVSNTGKIRQKSPTISKTSATEVFSMALQLYHDKEAAAA
                     SLMNGDGLLELLLGVAKELNNAD"
     gene            7683..>7886
                     /locus_tag="PN977_004702"
     CDS             7683..>7886
                     /locus_tag="PN977_004702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012368856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDI6044638.1"
                     /translation="MKQQVSFKFRLKPDGQQERQMRRFAGACRFVFNRALALQNENHE
                     AGKKYIPYTKMASWLVEWKKDTET"
BASE COUNT         2096 a         1965 c         1767 g         2058 t
ORIGIN      
        1 acaacctgcg acccgctgtc aaacaggtgg caggacgtga cagggttgac agactggcac
       61 gaatgaaacc agcaggccga agcctcccca tcacgcccca ccataattcg ggcgtaacgt
      121 ggtttacgga cacaaaaata ccgcaatatc ggaaatctgc ggttgtccgc attcgtattc
      181 aggctgtcaa acctggtcgc agaatttgct acgacggtgg aactataagc ctgaacgacg
      241 atgagatcaa tagagccgtg ttcgatggta ggttatttgc tctaataaat caaatttatt
      301 agagcaataa tggttaaatc atcttcgcct gcgtgccata tatttgcgca ccgcgcggcg
      361 tggacaatct gaagcggttt ctttctgctg catcaatctc gcagccatgc tcaaaaaagt
      421 caggcacagc cgaagcccgg catacaatag cggttccagt ggccacgttt cattaagcac
      481 atataccgcc atgaaaatcg agtcgaaaac tatcgctgcc agcgataact tcattgtcga
      541 aagtcggcgg agctgcctga gtttattcat tgaccagccc cgtcaggcaa agctggcgtt
      601 ctttttcacg gcgaatcttt aaacctcgca ggggcacgcc gttactgttc acgaaatcag
      661 ggagatggtt acacatattc acccattccc ctttctgcgc ccacttgtgg atggacgttt
      721 ctactcgcat gcctcgcgct ttgctgtagt aggtccgtaa gctattgcat cccatattga
      781 atgccgcgct tgtcattgca ctgaaggcat tatcgggcat gtctttgccc cggaagtgct
      841 gattaataca gcgttcagcg atcaggatat tcttttccca atcagcggcg atttgctggt
      901 cggtttttcg cacacccggc gttaccccgt gtgtattacc gatcccgtca gtccataccc
      961 ccgccgggca catgtatgga tcacgtcggc aaccttcagc gttaccaatc agctcaagcc
     1021 ccgcctggtt ggttcgcaca ttgccattac ccatcacgat ggtaatcatc accgcgatag
     1081 cgcaaattgc accgcctcct gcggctgttt ttcccttcat aaagacctca taagcgaatt
     1141 ttttacgctc caggacaaac actcattcac agccaatacc gactgactcg atccctttag
     1201 aaggcacagg ataatgcaaa tcacttgtta gccacgtttc aaagatatac attattgctc
     1261 taattaattt attttattag gtaagaaaag tggcacaacg cggtgtaaac aaagtcatcc
     1321 tgattggtac cctggggcaa gacccggaga tcaggtatat accaaatggc ggcgcagtcg
     1381 gaagactcag catcgcaacg aatgaatcat ggcgcgataa gcaaacgggc caacagaaag
     1441 agcaaacaga atggcataaa gtcgttttgt tcggaaaact tgctgaaatt gcgagtgagt
     1501 atttacgaaa aggttctcag gtctacatcg aagggaaact taaaacccgt aagtggacag
     1561 atgacgccgg tgtagaacgt tacacgacgg aaattatcgt cagccagggc ggcaccatgc
     1621 aaatgatcgg cgctcgccgt gacgattcac agtcctcaaa tggctggggg caatcaaacc
     1681 aacctcaaaa ccaccagcaa tacagtggtg gcggtaaacc tcagagcaac gccaataacg
     1741 aacctccaat ggactttgac gacgatattc cgttttgaat gtgtaaaaaa cgactgaaag
     1801 aaaagcggtg gtccagacgc cgacaaaagc acgaactcgc aaacaaacgc caaagttggc
     1861 aatggcacgc gtttttaacg aacagaacac cacgagatat tgctttctct ggtgggaaaa
     1921 cattcctaac ccacctgaag acgcaataca tcagttttta agcagagaaa agactatgac
     1981 agcacaaaat actaaaacca ttcaataccg cctacgtaat ggccagagtg tcgaagtgac
     2041 catcaataat gatggagtac ctggcgaaaa ggtttctatc tctgatctgg ctatcgaaaa
     2101 aaccatcatg tgccaccttg gctttactga agaagtgagc aaaaagcatg gtgtagcaat
     2161 ctggagcgca atggatactg gtatgcgcaa attcattact gctcgtaccc cagggatgac
     2221 catgatggac ctcatgcaga ttgcgccgct gtttgaatgt gagcctttag atgtattcag
     2281 caatccagct atctgccagc agttatatgg tgagatgaaa ctcgcggtta cccccattgt
     2341 gctgcatgaa ggatcgcttg ctggcgtgtg gaaagtagag cgtatttcaa gctacatgcc
     2401 tttccatgtc aacggcgtaa tcactggtga aaatcaacct gtttccgtta taaagtcaga
     2461 cctcaagcgc gcaattcttg aagcaagttg tcgagttgtc ggcctgggca aacagtctta
     2521 tgtttccttc ccggctggcc ctgaaggccc ggcagaaatt ctgattatgg atgccgatct
     2581 gctatggcaa atacagttta tgattggcaa aagcatcatc cgagctgaag aactcgatca
     2641 gtacattacc tgcacgatga ctgatgaagt caaaagtgtg gctacaacca atgcaaggag
     2701 cctgtttcgt gctgcattgg cagaaccgca agaaaacatt ccagaagaaa cagaaagtga
     2761 ctaataaaaa ccccgtcagt tggcggggaa ctccacgtca aatggccaat tgttatgtca
     2821 gctagcagtt aaagcgccta gtcatcatct tttaaagaag tctttttaac ttacctaaat
     2881 tagtgacacc tcccgttttg cttgctattg tacacattca tgaatctatt cattaatcta
     2941 ttcacaatag taattaaaac tatggctaca catgcaacaa caatccgtaa tttgctcaat
     3001 caaggtccta tgacaccaag acaattggtt gaaagactaa gtgttagcca accaacgata
     3061 tctagagcat taagagatct tggtgatgaa atcgttagga tcggaaatgg aagatctatt
     3121 caatatgctt tacgagactc ttttagagga ttcgaatcag tccccattta tcgtataact
     3181 gaagaggggt gtatttgcgt tcttggtact ctcattccgg tgaggccaga aggctatgtt
     3241 atggttgagg aagggaaaaa taacaggcat agcgatgggt taccttggtg gttatttgat
     3301 atgcgtccac aaggtttcct tggaagagca tatgcatcag catatgctct tgaacttaac
     3361 cttccctcaa atccagagca atggtcagat actgacgtga tcaaagcctt acttaagcat
     3421 gggcatgatg ttgttggtaa tttgttaatt ggagagcaag cacgcacgca ttttttagag
     3481 atgcagcctc ctgttcctgt taatcgtgaa agtgaatatc ctgtattagc tttagctgct
     3541 ggtgaaggtg atgtgcccgg ttcatctgct ggaggtgaac aacccaaatt ttgtactttc
     3601 acagagcgtg gacatgtatt agtgaaattc tctgcgccta acgacacccc tataagtgag
     3661 cgctggagag atctcttgtt tgctgaacat ctcgcattaa aagttctggg ggtagaaaca
     3721 gaagtttttg attataacgg tcagagattc ttggaaattc cccgatttga tcgtgttggt
     3781 caactcggtc gggtaggtgt attctcactt cgtgcacttg atgcagaatt tgttgggaat
     3841 gcaagagcaa catggccctt gctggttaaa gagttggtaa aaaataagtg cgtccatcct
     3901 gatgcgatta ttatagcaac aaggctatgg tcttttggtt tgctaatagg caataccgat
     3961 atgcataatg gtaacctatc gtttattagt agccacggac gcccatatca tattgctcca
     4021 gcctatgaca tgttacctat gggttttgct ccacgctctg ggggagcaat atctaatgag
     4081 cttagaaaaa cagcactacc agactttgta agtcgaaaaa tatgggatga atctcttgaa
     4141 ttggctgaaa aattttatgc attggcctgt gagtccgaca aattctcagc taactttact
     4201 ccatgtttag aagcgctacg catccaccta gatgattgta agatacgcat agcccgccta
     4261 gcataaacaa caaatcccgc cgattggcgg gatttcttca atatacgatc tggtttacat
     4321 gatcaccaaa atcatcgtcg tcgtcctcat cgccaccatc tactgctggc caatcaacaa
     4381 accagccagc gtaaagatgc agcgttcgga gaacatcact tgcgggagca tcaagggtgt
     4441 taacgaatcc catatagcta ttgggatttg cccctgctat ggcttcagcg atcatgtcct
     4501 cggtaatgtc accggagata atgcttaaac gcccggaaac ttcttcatta tcatcaaatt
     4561 cgataatggc atctccacct aatggcgctg cgattttaat ctgcattatt tagctccttt
     4621 gccacaccta ataacagttc cagcaatccg tcaccattca tcagtgatgc ggcagcggcc
     4681 tctttgtcat gatacaactg aagagccata gagaatactt ccgttgctga cgttttggaa
     4741 atagtcggtg atttctgccg aattttcccg gtgttactta ctgaggctgg cgggtatacc
     4801 ttcgccatat aaatattact caatcgagat ctgaagcacc attcaggctt gccacgccca
     4861 ccgatattga cgaaagatgg cttatcccct tcaacattgg ccttcaggaa tgaccgggct
     4921 ttctctaaca aaccagggtt actgtactca agatgatgac ccagctcgtg ccacagtgca
     4981 cttgcatttt catcgttcaa attgacagca acaacaccat ttagatttgc atatgccctt
     5041 ccctggtggt gaaccacttt tgataaggtc gatattttcc cgccggtcag gcgataaata
     5101 tcagcaagtt ccttgcgcag gtctatccca ccattctgtc cagcgcgggc ttcttccact
     5161 tcttctgtga taaaagagtc ggcccactca agagcttttt cttcagatac ggatgagttt
     5221 gcgatcgcac tgttcatggc agataacact ttctcgtgga ccgaacccat acttcgctga
     5281 ttcatttgcc agcgtgtctg cgggttatat gagaatcgct taagtagttg gtcaagctgc
     5341 tcaagttctt cttcactgac atacttttta gcctcaccaa taatgccagg gagaatattg
     5401 ccgttaggat taaacgctcg cgaaaggaag agtttcagcg cccccatgcc ctccgatgct
     5461 tcaatatcac caataacccg gttaacaatg gccgcactct tcggattagc atccgccaac
     5521 gctctggcta caatttgcag ggacgatacg acctcacgct gcatatccgt cctgatctca
     5581 tcaataaact ctggtgttat gccgtgctct ttaaggatat cccggccttc cgccgttacc
     5641 ccatcgatat caccgacatg tttattaaca cgactttgca atgccttaaa tgccttcaga
     5701 attccacggg catcatccgc tttactaacg gccttcctga atgctggcaa gaagtctgag
     5761 ttaacctcat tttgttgatc ggcccactga atggaggctt ctttcatctc gtccagagtc
     5821 agatcaccca acgcggtatg gtctgtgaat atgagcgaca acctctgaac catttctgcc
     5881 aatggtgatg ccgaatgcgc cgcgctaagg aatgctttca ccctggttgg gcgaatggaa
     5941 aaccagtcaa tagctggtgg catatctccg ttttttatcg cctgcgctat ctcgtcaaag
     6001 ccatcgcgcc caagggagga tgcgtgattt aacaagccgc gaagtaacga attgctgata
     6061 ccgaataatc ggcaccattt tttcacgtcg gcaacaggca ttcgaacaaa atgcgcaagc
     6121 acttgtacaa gctgttcatc ctggggatct gtgcgggaaa gcagcctgat cagatgaata
     6181 atgtctttga tgccggatgt ccgatgtaat agcaaactgg tatacggagc aacaccgttg
     6241 taactaccgc cggaaacgga ctcgaaaaga ccgccggata tcccttgcat gccttcgttt
     6301 tccagttcct gagacacctg gcgaaggata tcctgtaacg acacatcgcc gccgccaaac
     6361 atatccccca gcgcctggcc ctggtgctgt aactcatcat tgatacgttg agccatcaac
     6421 ttaaaggcgg tggccatacg cttcgcgcta cggttattcg cgacgatgaa caacgcgagt
     6481 gctttcactt ccggggccat ttcgctgaac atatccccct gagcaataac atcggtaata
     6541 tgctggcctg actccttcga ttgccttacc aggtctaccg catctttcaa tgccgccagc
     6601 gcctttttat cgaggctatc cgctgtctca atgccatcaa caatagttgt cacagcctgc
     6661 ttgtgcgctt ctcctgataa agcctgcatc tggacaaaat cattggctgc cgcattaagc
     6721 gccgtcagaa cattacgcat atccggatca ggttcttctg caaccattct taccaggcgc
     6781 gcatccttat atgccttggc aaagatcgcg ttttgtatac ggtcaacaag ttgccgtgtt
     6841 ggtcgcccat cttccgttac aaggccagca gcctgtgtgg caccaacttg cgtcatgaat
     6901 ccgcgaataa acgcgtcatt actgcggcta agcagatctc cgctttctga tgggttaaac
     6961 agcgccatca tcgccggagt tatgctgtcg gcatcaacaa aagccttttc actggctgcc
     7021 atttcctgga gatcagaaat atttgagtcc ttggcaaact gaacacggtc aaccttcgtt
     7081 aaccggcggc gcaccagtac cggagccgtc attgattcaa ccttttcagg tcgtattccg
     7141 aattcggtcg catgttcaat caggtactcc cgataccgat ccgcattacc gtcctgatag
     7201 gctttaatga tccccatggt cctcccatta cctgactcaa cggcattgtc ctcaccaatt
     7261 atcggcgcgc catggctgga taaaccggaa tcagtaagct gagcaggccg caaatctttg
     7321 gatatctggt taacctgaag aaggctggat gcgcgggtcc ggtcgcgcgg ttgaagttcc
     7381 tggggatagt caggattaat tttcccatcc agagtattgg ataccaaaag agctgaggca
     7441 tcgacgatat caaacgctgt ttttacctcg tctcccttcg ctgtcaccac atacgaaacc
     7501 cgcccataat cgggcaggtt ctttagcagc tcgatcagcg tttctatgct ggtggccatt
     7561 accacctgat cgcttaagct catccctgtt acgccttatc caatgtgccg ccccctacgc
     7621 tcctcatagt ggggatataa ggcacagttt tcctcccggc tatttttgct taaaatagtg
     7681 acatgaaaca acaggtgtca ttcaaattcc ggttaaaacc agatggtcaa caggagcgtc
     7741 aaatgaggcg ctttgccgga gcttgtcgtt tcgttttcaa ccgtgctctg gcgcttcaga
     7801 atgagaatca tgaggccggt aaaaaataca tcccttacac gaaaatggct tcctggttgg
     7861 ttgagtggaa aaaagacact gaaacc
//
LOCUS       DAMZFT010000196         7814 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.196, whole genome shotgun sequence.
ACCESSION   DAMZFT010000196 DAMZFT010000000
VERSION     DAMZFT010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7814)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7814)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7814
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.196"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(<1..205)
                     /locus_tag="PN977_004703"
     CDS             complement(<1..205)
                     /locus_tag="PN977_004703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-mannonate oxidoreductase"
                     /protein_id="HDI6044639.1"
                     /translation="MGNNLLSAKATLPVYDRNNLAPRIVHLGFGAFHRAHQGVYADIL
                     ATEHFSDWGYYEVNLIGGEQQIAD"
     gene            complement(294..1661)
                     /gene="ydfJ"
                     /locus_tag="PN977_004704"
     CDS             complement(294..1661)
                     /gene="ydfJ"
                     /locus_tag="PN977_004704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000582800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MHS family MFS transporter YdfJ"
                     /protein_id="HDI6044640.1"
                     /translation="MTIEKHERSTKDLVKAAVSGWLGTALEFMDFQLYSLGAALVFHE
                     IFFPESSTAMALILAMGTYGAGYVARIVGAFIFGKMGDRIGRKKVLFITITMMGICTT
                     LIGVLPTYAQIGVFAPILLVTLRIIQGLGAGAEISGAGTMLAEYAPKGKRGIISSFVA
                     MGTNCGTLSATAIWAFMFFILSKEELLAWGWRIPFLASVVVMVFAIWLRMNLKESPVF
                     EKVNDSNQPTAKPAPAGSMFQSKSFWLATGLRFGQAGNSGLIQTFLAGYLVQTLLFNK
                     AIPTDALMISSILGFMTIPFLGWLSDKIGRRIPYIIMNTSAIVLAWPMLSIIVDKSYA
                     PSTIMVALIVIHNCAVLGLFALENITMAEMFGCKNRFTRMAISKEIGGLIASGFGPIL
                     AGIFCTMTESWYPIAIMIMAYSVIGLISALKMPEVKDRDLSALEDAAEDQPHVVRAAQ
                     PSRSL"
     gene            complement(1719..2738)
                     /locus_tag="PN977_004705"
                     /pseudo
     CDS             complement(1719..2738)
                     /locus_tag="PN977_004705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416097.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-dependent oxidoreductase"
     gene            complement(2750..3964)
                     /gene="rspA"
                     /locus_tag="PN977_004706"
     CDS             complement(2750..3964)
                     /gene="rspA"
                     /locus_tag="PN977_004706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="starvation-sensing protein RspA"
                     /protein_id="HDI6044641.1"
                     /translation="MKIVKAEVFVTCPGRNFVTLKITTEDGITGLGDATLNGRELSVA
                     SYLQDHLCPQLIGRDAHRIEDIWQFFYKGAYWRRGPVTMSAISAVDMALWDIKAKAAD
                     MPLYQLLGGASREGVMVYCHTTGHSIDEALDDYARHQELGFKAIRVQCGIPGMKTTYG
                     MSKGKGLAYEPATKGQWPEEQLWSTEKYLDFMPKLFDAVRNKFGFNEHLLHDMHHRLT
                     PIEAARFGKSIEDYRMFWMEDPTPAENQECFRLIRQHTVTPIAVGEVFNSIWDCKQLI
                     EEQLIDYIRTTLTHAGGITGMRRIADFASLYQVRTGSHGPSDLSPVCMAAALHFDLWV
                     PNFGVQEYMGYSEQMLEVFPHNWTFDNGYMHPGDKPGLGIEFDEKLAAKYPYEPAYLP
                     VARLEDGTLWNW"
     gene            complement(4170..4496)
                     /locus_tag="PN977_004707"
     CDS             complement(4170..4496)
                     /locus_tag="PN977_004707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnfA family protein"
                     /protein_id="HDI6044642.1"
                     /translation="MIKTTLLFFATALCEIIGCFLPWLWLKRNASIWLLLPAGISLAL
                     FVWLLTLHPAASGRVYAAYGGVYVCTALIWLRVVDGVKLSLYDWTGALIALCGMLIIV
                     AGWGRT"
     gene            4631..4972
                     /locus_tag="PN977_004708"
     CDS             4631..4972
                     /locus_tag="PN977_004708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1283 family protein"
                     /protein_id="HDI6044643.1"
                     /translation="MKITLSKRIGLLAFLLPCALALSTTVHAETNKLVIESGDSAQSR
                     QHAAMEKEQWNDTRNLRQKVNKRTEKEWDKADAAFDNRDKCEQSANINAYWEPNTLRC
                     LDRRTGRVITP"
     gene            5007..5567
                     /gene="speG"
                     /locus_tag="PN977_004709"
     CDS             5007..5567
                     /gene="speG"
                     /locus_tag="PN977_004709"
                     /EC_number="2.3.1.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine N1-acetyltransferase"
                     /protein_id="HDI6044644.1"
                     /translation="MPSAHSVKLRPLEREDLRYVHQLDNNASVMRYWFEEPYEAFVEL
                     SDLYDKHIHDQSERRFVVECDGEKAGLVELVEINHVHRRAEFQIIISPEYQGKGLATR
                     AAKLAMDYGFTVLNLYKLYLIVDKENEKAIHIYRKLGFTVEGELMHEFFINGQYRNAI
                     RMCIFQHQYLAEHKTPGQTLLKPTAQ"
     gene            complement(5570..6280)
                     /locus_tag="PN977_004710"
     CDS             complement(5570..6280)
                     /locus_tag="PN977_004710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416102.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnfC family lipoprotein"
                     /protein_id="HDI6044645.1"
                     /translation="MKYKLLPCLLAIFLTGCDRTEVTLSFTPEMASFSNEFDFDPLRG
                     PVKDFTQTLMDEQGEVTKRVSGTLSEEGCFDSLELLDLENNTVVALVLDANYYRDAQT
                     LEKRVRLQGKCQLAELPSAGVSWETDDNGFVIKASSKQMQMEYRYDDQGYPLGKTTKS
                     NDKTLSVSATPSTDPIKKLDYTAVTLLNNQRVGNVKQSCEYDSHANPVDCQLIIVDEG
                     VKPAVERVYTIKNTIDYY"
     gene            6388..6693
                     /locus_tag="PN977_004711"
     CDS             6388..6693
                     /locus_tag="PN977_004711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1161 domain-containing protein"
                     /protein_id="HDI6044646.1"
                     /translation="MKLSTCCAALLLALASPAVLAAPGSCERIQSDISQRIINNGVPE
                     SSFTLSIVPNDQVDQPDSQVVGHCANDTHKILYTRTTSGNVSAPAQSTQDGAPAEPQ"
     gene            6892..>7814
                     /locus_tag="PN977_004712"
     CDS             6892..>7814
                     /locus_tag="PN977_004712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HDI6044647.1"
                     /translation="MSKNEQMVGISRRTLVKSTAIGSLALAAGGFSLPFTLRSSAAAV
                     QQASEKVVWGACSVNCGSRCALRLHVKDNEVTWVETDNTGNDEYGNQQVRACLRGRSI
                     RRRINHPDRLNYPMKRVGKRGEGKFERISWDEALDTIASSLKKTVEQYGNEAVYIQYS
                     SGIVGGNMTRSSPSASAVKRLMNCYGGSLNQYGSYSTAQISCAMPYTYGSNDGNSTTD
                     IENSKLVVMFGNNPAETRMSGGGITYLLEKAREKSNAKMIVIDPRYTDTAAGREDEWL
                     PIRPGTDAALVAGIAWVLINENLVDQPFLDK"
BASE COUNT         2176 a         1970 c         1710 g         1958 t
ORIGIN      
        1 aatcggcaat ttgctgttcg ccgccgatta aattgacctc ataatatccc cagtcactga
       61 aatgttccgt agcaagaata tcggcataca caccctgatg cgcacggtga aatgcaccaa
      121 agcctaaatg aacaattctt ggggccaggt tattacgatc ataaacaggg agcgtcgctt
      181 ttgctgataa caaattattt cccataacaa ttccttaaat ataaatatgg caagctacat
      241 gttttgttat atgaataaaa atcccctctc cggtaagaga agggatggag gatttaaaga
      301 cttctggaag gttgcgcagc tctgacaaca tgcggttgat cttccgcagc gtcttccagc
      361 gcacttaaat cacggtcttt cacttctggc attttcagcg cagaaattaa accaatcact
      421 gaatatgcca tgatcataat tgcgatcgga taccaggatt ccgtcatggt gcagaaaata
      481 cccgccagga taggaccaaa accggaagcg ataagaccac caatttcttt agaaatggcc
      541 atccgggtaa agcggttttt acagccgaac atttctgcca tggtaatgtt ttccagagca
      601 aataatccca gcaccgcaca gttatgaatc acaatcagtg caaccataat ggtgctcggg
      661 gcatagcttt tatctacgat gatagaaagc attggccatg ccagcacaat cgcagaggta
      721 ttcataataa tatacgggat ccggcgacca attttatcgg ataaccaacc aaggaacgga
      781 atggtcataa agccgagaat cgaactgatc atcaatgcat ctgttggaat tgctttgtta
      841 aacaataacg tctgcactaa atagcctgca aggaaagtct gaattaaacc tgagttacca
      901 gcctgaccaa aacgcagccc tgttgccagc cagaaggatt tgctctggaa catgctacca
      961 gcaggtgcag gttttgctgt aggttggttg ctgtcgttaa ccttctcaaa gaccgggctt
     1021 tctttcagat tcatacgcaa ccagatagca aagaccatca cgacaacgct cgccaggaac
     1081 ggtatacgcc atccccacgc caacagttcc tctttactga gaatgaagaa cataaaggcc
     1141 cagattgccg ttgcgctcaa ggttccgcag ttagtcccca tagcaacaaa tgaggagata
     1201 attccgcgct taccttttgg cgcatattcc gccagcatcg taccggcacc ggaaatttcc
     1261 gcgcctgccc ccagcccctg aataatacgc agcgtcacca gcaaaattgg cgcaaacaca
     1321 ccaatttgtg cataggtcgg cagcacacca attaaggtgg tacagatccc catcatagtt
     1381 atggtaataa agagcacttt tttacgcccg attctgtcgc ccattttgcc gaaaataaat
     1441 gctccgacaa tacgcgccac ataacctgca ccgtaggttc ccattgccag aattaacgcc
     1501 attgccgttg atgattcagg aaaaaatatt tcatgaaaca ctaacgctgc gccgagcgaa
     1561 tataactgga aatccataaa ttcaagcgca gtgcccagcc atcccgatac tgctgctttc
     1621 accaaatcct tagtgcttct ttcgtgtttt tctattgtca taatggttat ctctaaaaaa
     1681 gaggtaagat gcgtactact tactcgccgt tattggtatt attcagaaaa agtgagtaag
     1741 actttgcagc aatgcttttg atcctgttca aataaactaa tggcatcagc aacatgctgg
     1801 aaatcaaacg tatgggtaat tagtttttct ggtttaatta accctttact taaccagtcg
     1861 ataacgaccg ggaatttatt tgcatttaag cgtgaagaga aaatagagag ttcttttccg
     1921 gtaattcctt gctgaatcac ttcagacggt tcactggaga agcccatcaa tacaatacgt
     1981 gccgctggag aagccagcgt tacggcttct ttcaggattg aaggatgaca agccgcatcg
     2041 ataattaatg tcggcttgat gcctttttca gcgaaactct cgctaagcgg tgtctggctg
     2101 ttattaatcg cccagtctgc cccgctctct tacgcttttt ccagtcgttc atcaatgcga
     2161 tcggcaacaa tcacattctt aacgttatag acgcctttta atacctgaac gatcgtcagg
     2221 ccgattggac ctgcaccgta aaccagaacg gtatcatttt cagtcggttg accatgaccg
     2281 gtaacgttag ccgcaatggt aaaaggttca atcatcaccg catattgatc ggccactgct
     2341 tcaggaattt tccacgcatt ttttgccggc accacggcat attcactgaa accaccgtca
     2401 gcgtgcacac ctaatacagc cagtgtcgta caaacgttcg gcttacctat agagcacgga
     2461 tagcaatgcc cacagctgac caccggatcg acagcaacgc gttcaccgac tctgtcgctt
     2521 tccacgcctt cacccactgc atcaatgacg ccaaagaatt catgtccaat gacgcgcgga
     2581 tatttcgcaa aaggattatg cccacgataa atatggctat ctgaaccaca aattccggca
     2641 agtttcactt ttactcgtac ttcacccgct gacggggtgg gtatttcgcg ttcgataatt
     2701 gccagttgat tcggtttttc aattaaaatg cttttcatta tcttactcct taccagttcc
     2761 acagcgtgcc atcttccaga cgtgcgactg gcagataagc aggttcatag ggatatttcg
     2821 ccgccagctt ttcatcgaat tcgatgccaa gacccggttt gtctcccgga tgcatatagc
     2881 cgttatcgaa agtccagttg tgcgggaaga cttcaagcat ttgttcggaa taacccatgt
     2941 attcctggac accgaaattg gggacccaca gatcaaagtg cagcgccgca gccatgcaga
     3001 ctggcgacaa atcggaagga ccgtgtgagc cagtacgtac ctgatacagc gaagcaaaat
     3061 cggcaatccg gcgcataccg gtaatccccc ccgcgtgagt cagcgtggtg cggatatagt
     3121 cgatgagttg ttcttcaatc agctgtttgc agtcccagat gctgttgaag acttcaccca
     3181 ctgcaattgg cgtgacggtg tgttggcgaa tcaggcggaa acactcctgg ttttccgcag
     3241 gcgtcgggtc ttccatccag aacatgcgat aatcttcaat gcttttacca aagcgcgccg
     3301 cttcaatagg cgttaagcga tggtgcatgt catgaagcaa atgttcatta aaaccaaact
     3361 tgttacgtac cgcgtcaaac aatttcggca tgaaatcgag gtatttctcc gtcgaccaca
     3421 gctgctcttc cggccactgt cctttggttg cgggttcata agccagacct ttacctttcg
     3481 acatgccata ggtggttttc ataccaggga ttccgcactg cacgcggatg gctttgaatc
     3541 ccagctcctg atgacgggca taatcatcca gagcttcatc aatactgtga ccggtggtat
     3601 ggcaataaac catcacccct tcacgagacg cgccgccgag taactggtaa agcggcatgt
     3661 cggcagcttt ggctttaata tcccacagcg ccatatcaac cgctgaaatg gccgacatcg
     3721 taaccggacc gcgacgccag taagcacctt tatagaaaaa ctgccagata tcttcgatac
     3781 ggtgtgcatc gcgaccaata agctgcggac aaaggtgatc ctgcaaataa gaggccacgg
     3841 aaagctcacg tccatttagg gtggcatccc caaggcccgt aataccgtcc tcagtggtga
     3901 tttttaatgt gacgaaatta cgccccggac aggtaacaaa aacttcagcc tttacgatct
     3961 tcatgttcga ttccttgcat cgcttgtcgt gatgcatgaa atttacgcaa ctgagctact
     4021 accatacaag tatgaagatc aaaaaaagcc ggagtgatca caaaaaaagg cgtatatttg
     4081 cgccgtgaat ggttgacaaa agatgaaata gaataccttt tgtcagctga cacttcctcg
     4141 tatcttattg ataaaatgaa tttatgttcc tacgtgcgcc cccagcccgc tacaatgatc
     4201 aacatgccgc aaagcgcaat caacgccccc gtccagtcat aaagactcag tttcacgcca
     4261 tccacaacgc gtagccaaat caacgccgtg cagacataaa cgccaccata agccgcgtaa
     4321 acacgcccac tcgccgcagg atgcaacgtt aacaaccaga caaacagcgc cagtgaaatc
     4381 cctgccggaa gcaacagcca gatactggcg tttcgcttca accacagcca gggcaaaaag
     4441 cacccaataa tttcacacag cgcagtagca aaaaatagta acgttgtttt aatcatcttt
     4501 gtcacttatt gacatcatgt atagttatag gacgacataa tatcagcaat ataaacaccc
     4561 tccttgaacg ttttgcgttc gcagtggatg gtgtagaatc agctttaatc attcatacag
     4621 ggaatgaatt atgaaaatca ctctcagcaa acgaatcggc ctgctcgctt ttctgctgcc
     4681 ttgcgcactg gcattgagca caactgttca tgccgaaact aacaaactgg tgattgagtc
     4741 tggcgacagt gcacaaagcc gccagcacgc cgctatggaa aaagagcaat ggaatgacac
     4801 gcgcaatctg cgccagaaag tgaataaacg cactgaaaaa gagtgggata aagccgacgc
     4861 cgcttttgat aaccgcgata aatgtgagca aagcgccaac atcaatgcct actgggagcc
     4921 aaatactttg cgctgcctgg accgtcgaac tggtcgcgtt attaccccct aacctgttat
     4981 tgatttaagg aatgtaagga cacgttatgc caagcgccca cagtgttaag ctacgcccgc
     5041 tggagcgtga agatttacgc tatgtacatc aactcgacaa taacgccagt gtgatgcgtt
     5101 actggtttga ggaaccctac gaagcctttg ttgaactctc tgatctgtac gataagcata
     5161 ttcacgatca gagcgaacgg cgctttgtgg tggaatgtga cggcgaaaaa gccggtctgg
     5221 tggagctggt ggaaattaac catgttcatc gccgcgcaga atttcagata attatctccc
     5281 cggaatatca ggggaaaggt ctggcaacgc gtgccgcaaa attagcaatg gactatggct
     5341 tcaccgttct caatctctat aagctgtatc tgatcgtcga taaagagaat gaaaaagcga
     5401 ttcacattta ccgcaagctt ggctttacgg ttgaaggtga attgatgcat gagttcttta
     5461 ttaatggtca atatcggaat gccattcgta tgtgcatatt ccagcatcag tatctggcag
     5521 agcacaaaac accgggtcag actctcctga agccgaccgc acaatagcat taataataat
     5581 cgatagtatt tttgatggtg taaacccgtt cgacggcggg ttttactcct tcatcaacaa
     5641 tgattagctg acagtctacc ggattggcgt gactgtcata ttcacagctc tgttttacat
     5701 taccaacccg ttgattattc agtaaagtaa ccgcggtgta atctaatttt ttgatcggat
     5761 ccgttgatgg cgtggcgctg acagataatg ttttgtcgtt acttttcgta gttttaccca
     5821 gcggataccc ctgatcatca tagcgatatt ccatctgcat ctgtttgctg ctggctttaa
     5881 tcacaaagcc attatcatcg gtttcccaac tcaccccggc agaaggtaat tctgctagct
     5941 ggcattttcc ctgcaaacga actcttttct ccagtgtctg ggcatcacgg taataattgg
     6001 cgtccagtac cagggcaacc acggtattat tttccagatc cagtaattcg agagaatcaa
     6061 aacagccttc ttccgacaaa gtccccgaaa cacgtttcgt cacttcacct tgctcatcca
     6121 ttaatgtctg agtgaaatct tttacgggac cacgcagcgg atcaaaatcg aattcattag
     6181 agaaactggc catctccggg gtaaatgaaa gtgttacctc tgtgcggtca catcctgtga
     6241 ggaatatcgc gagtaagcat ggcagtaatt tgtatttcac aacagtcacc tgagagtaga
     6301 gatgattctc aatcatagta gcaaatacag tactttacac gttaaatgct atgcttaaag
     6361 aagttatctt cgcgtaagga gcttatgatg aaactctcaa cctgctgtgc cgcgcttctg
     6421 ctcgccctcg cctcacccgc ggtactcgcc gcgccgggat cctgtgagcg catacagagc
     6481 gatatatcac agcgcattat caataatggc gtacccgaaa gcagcttcac gttaagtatt
     6541 gtacccaatg accaggttga tcagcctgat tcccaggtcg tcgggcattg cgctaatgat
     6601 acgcataaaa ttctctacac ccgcaccacc agcggtaacg tctctgctcc cgcgcagtct
     6661 acccaggatg gcgcgcctgc cgaaccgcag taacacatta tcgactgaac gccggatacg
     6721 acacatccgg cgatttgaac atacaacata atcccacctt attactcata cccttctatt
     6781 gatatggatt aataattctt aacccaaaat gggtagactc cctctattgt tagcgcgcta
     6841 aatattcaat atataaactt ttatataacg ataaagaaca gggagtgagt tatgtccaaa
     6901 aatgaacaaa tggtgggcat cagccgcaga acactcgtta aatctaccgc gataggttct
     6961 ctggcgctgg ctgcaggcgg tttttcttta ccgttcaccc tgcgcagttc agcggcagcg
     7021 gtacaacagg ccagcgaaaa agtggtctgg ggtgcctgtt ccgtcaactg tggtagccgc
     7081 tgtgcacttc gtctacatgt taaagataat gaagtcactt gggtggaaac tgacaacacc
     7141 ggcaacgacg agtatggcaa tcaacaagta cgcgcctgtt tgcgcggtcg gtccatccgt
     7201 cgacgtatta atcatcccga tcgcttgaat tacccaatga aacgcgtggg caaacgtggc
     7261 gaaggcaaat tcgaacggat tagctgggat gaagccctgg atactatcgc cagtagcctg
     7321 aaaaaaaccg tcgaacaata tggcaatgag gctgtatata ttcagtactc ttcagggatc
     7381 gttggcggca atatgacccg ctcttcacca tcagcctcgg cggtcaaacg cctgatgaac
     7441 tgctacggcg gttcactcaa tcagtatggc tcctacagca ccgcgcaaat ttcctgtgcc
     7501 atgccctaca cctacggcag taatgatggc aacagcacca cggatattga aaacagcaag
     7561 ctggtcgtga tgtttggtaa caacccggca gaaacccgca tgagcggcgg tggcatcact
     7621 tatcttcttg aaaaagcacg cgagaaatcg aacgcaaaaa tgattgttat cgatccgcga
     7681 tataccgata cggctgctgg tcgtgaagac gaatggttac ctattcgccc gggcaccgat
     7741 gccgcgctgg ttgcgggtat tgcctgggta ttgattaacg aaaatctcgt tgatcaacct
     7801 tttctcgata aata
//
LOCUS       DAMZFT010000197         7754 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.197, whole genome shotgun sequence.
ACCESSION   DAMZFT010000197 DAMZFT010000000
VERSION     DAMZFT010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7754)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7754)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7754
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.197"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(<1..3758)
                     /gene="air"
                     /locus_tag="PN977_004713"
     CDS             complement(<1..3758)
                     /gene="air"
                     /locus_tag="PN977_004713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter adhesin EaeX/Air"
                     /protein_id="HDI6044648.1"
                     /translation="MAGKAHGNGDRRGDNTICGLGDRLRRLTAGICLITQTIFPVMAA
                     APTHINSAHSDTAASLILPNVKTIPYTLGALESPPTVAARFGITVDELRRLNQFRTFA
                     RGFDNVRQGDEIDVPLINSNSPEARNLKAMQMERDGKDPQMQVAEMAQQSGTLLARDM
                     DSEQAASMARGWVASSASAQATDWLSRWGTARVSLGVDEDFSLKSSSFEFLHPWYETP
                     DNLVFSQHTLHRTDDRTQTNHGIGWRYFTSSWMSGVNMFIDHDLTRYHTRTGMGVEYW
                     RDYLKLSGNGYLRLSNWRSAPELDNDYEARPANGWDLRAEGWLPAWPQLGGKLVYEQY
                     YGDEVALFGKDERQNDPHAITAGLSYTPVPLISFSAEQRQGKQGENDTRIGMELTLQP
                     GHSLQKQLDPAEVAARRSLVGSRYDLVDRNNNIVLEYRKKELVRLTLTDPLKGKPGEV
                     KSLVSSLQTKYALKGYDIEAASLQSAGGKVAVSGKDIQVTIPPYRFTAMPETDNTYPI
                     AVTAEDSKGNFSRREESMVVVEKPTLSLTDSTLSVDQQILLADGKSTSTLTYTARDSS
                     GKPIPGMTLKTQVKGLQDFALSEWKDNGNGTYTQIVTAGKTSGALSLMPQFNGDDIAK
                     TPALIAIVANTASRADSTIETDQDNYVAGKPIVVKVTLRDDNGNGVTGRKELLKQTVK
                     VDNTKADDVSAWTEESEGIYKASYTAHLIGDKLTAQLTMPGWQTKHSDAFSIAGDKDT
                     AKIAAMQITANNAVARRDHNTVAVTVRDVHQNLLQGQNVTFTVVNGAAVFADPNGGIV
                     TTDKDGIASVNLASDQAVNSLIKAEINGSSQSVEVSFITGDISQLTSTIKTDDVSYTA
                     GGKIKVSVTLMDEQKNLVKGMASLLAGSSVVEVSGTDKNETGNWSEESDGVYTTTRTA
                     KIAGDRHYATLKLSTWSSAQQSDAYAIRESGAVLAYSSIVTDKTAYTAGGAIKVTVTL
                     KDSYENLVGGQRDAINLAIQLPNTKAESIAWNEDQKGIYTATYTALLPGTGLKAQLQM
                     SGWANALTSNDYSISGDAASAQIVAMQVTTGNPDVLANGSDRHTVNVRVEDQFGNVLS
                     EQTVTFTVTKGAAVFANAGQSADIRTDAHGMAEVDLSSTVADASTVEAKINQSSDSKT
                     VNFVADVSTAQVAELVVTQDGSVADGSTANTLRARVTDVFGNALAGQTVSVLAGNGAT
                     TAPTVTTQPDGTVEISVTSQTAGTSVITASVNNSSQSRDVTFIADVRTA"
     gene            complement(3961..5145)
                     /gene="nepI"
                     /locus_tag="PN977_004714"
     CDS             complement(3961..5145)
                     /gene="nepI"
                     /locus_tag="PN977_004714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine ribonucleoside efflux pump NepI"
                     /protein_id="HDI6044649.1"
                     /translation="MSEFIAENRGADVITRPNWSAVFSVAFCVACLIIVEFLPVSLLT
                     PMAQDLGISEGVAGQSVTVTAFVAMFASLFITQTIQATDRRYVVILFAVLLTLSCLLV
                     SFANSFSLLLIGRACLGLALGGFWAMSASLTMRLVPPRTVPKALSVIFGAVSIALVIA
                     APLGSFLGELIGWRNVFNAAAVMGVLCIFWIIKSLPSLPGEPSHQKQNTFRLLQRPGV
                     MAGMIAIFMSFAGQFAFFTYIRPVYMNLAGFGVDGLTLVLLSFGIASFIGTSLSSFIL
                     KRSVKLALAGAPLILAVSALVLTLWGSDKIVATGVAIIWGLTFALVPVGWSTWITRSL
                     ADQAEKAGSIQVAVIQLANTCGAAIGGYALDNIGLTSPLMLSGTLMLLTALLVTAKVK
                     MK"
     gene            5379..5732
                     /locus_tag="PN977_004715"
     CDS             5379..5732
                     /locus_tag="PN977_004715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HDI6044650.1"
                     /translation="MFKTKWFAREARSHAMTDEELCRAILETEQGKADVLGGGVFKKR
                     LHQNRERAIILAKGGSNWFYTFLYAKQDMSNINSQELAGFRELAKHYAFLTKAQLTAM
                     INTKELTEICYDCKN"
     gene            5716..6039
                     /locus_tag="PN977_004716"
     CDS             5716..6039
                     /locus_tag="PN977_004716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
                     /protein_id="HDI6044651.1"
                     /translation="MTAKTKFKSPAFEAIHSAAAGLSSVDAISAETMRTFDKACLTSV
                     QDLQPVEIKALREKLKVSQPVFAHYLNTSVSTVQKWESGAKRPNGISLKLLSIVQKHG
                     LEVLL"
     gene            complement(6155..6607)
                     /locus_tag="PN977_004717"
     CDS             complement(6155..6607)
                     /locus_tag="PN977_004717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1198 domain-containing protein"
                     /protein_id="HDI6044652.1"
                     /translation="MIWIMLATLAVVFVIGFRVLTSGARKAIRRLSDRLNIDVVPVES
                     MVDQMGKSAGDEFLRYLHRPDESHLQNAAQVLLIWQIVIVDGSEQNLLQWHRILQKAR
                     LAAPITDAQVRLALGFLRETEPEMQDINAFQMRYNAFFQPAEGVHWLH"
     gene            complement(6660..>7754)
                     /gene="adeQ"
                     /locus_tag="PN977_004718"
                     /gene_synonym="yicO"
     CDS             complement(6660..>7754)
                     /gene="adeQ"
                     /locus_tag="PN977_004718"
                     /gene_synonym="yicO"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeQ"
                     /protein_id="HDI6044653.1"
                     /translation="IFANLPVALAPAMGLNAFFAFVVVGAMGISWQTGMGAIFWGAVG
                     LFLLTLFRIRYWMISNIPLSLRIGITSGIGLFIALMGLKNTGVIVANKDTLVMIGDLS
                     SHGVLLGILGFFIITVLSSRHFHAAVLVSIVVTSCCGLFFGDVHFSGVYSIPPDISGV
                     IGEVDLSGALTLELAGIIFSFMLINLFDSSGTLIGVTDKAGLIDSNGKFPNMNKALYV
                     DSVSSVAGAFIGTSSVTAYIESTSGVAVGGRTGLTAVVVGVMFLLVMFFSPLVAMVPP
                     YATAGALIFVGVLMTSSLARVNWDDFTESVPAFITTVMMPFTFSITEGIALGFMSYCI
                     MKVCTGRWRDLNLCVVVVAALFALKIILVD"
BASE COUNT         2006 a         2101 c         1852 g         1795 t
ORIGIN      
        1 gccgtcctga catcggcgat aaacgtcaca tcccggctct ggctgctgtt gttgacgctg
       61 gcggtgatca cactggttcc ggcggtctgg ctggtgacac tgatctccac cgtgccatcc
      121 ggctgcgtgg tgaccgtcgg agcagtcgtt gcgccgttgc ctgccaacac gctaaccgtc
      181 tgcccggcaa gtgcattccc gaacacatcg gtgaccctcg cccgcagcgt gttcgccgtc
      241 gaaccatcag ccactgagcc gtcctgcgtc accaccagct ccgctacttg cgccgtgcta
      301 acatcagcaa caaagttcac cgttttacta tcgctgcttt gattgatctt agcctcaaca
      361 gtgctggcat ccgctacggt actgctcaga tcaacctctg ccatgccatg cgcatccgtc
      421 ctgatatctg cgctctgccc tgcattggca aatacagccg cccccttcgt caccgtaaag
      481 gtgacggtct gctccgacag cacattgcca aactgatctt ccacccggac attcaccgta
      541 tgtcgatcgc tgccattcgc cagcacatcc gggttacccg ttgtcacttg catcgcaaca
      601 atttgtgcgc tggcggcatc gccactaata ctgtaatcat ttgaagtcag agcgttcgcc
      661 cagccggaca tctgtagctg tgcttttaaa ccggtgccag ggagtaaggc ggtatacgtt
      721 gcggtataaa ttcctttttg atcttcattc caggcaatgc tttccgcttt tgtatttggc
      781 agctggatag ccaggttgat agcgtctcgt tgccccccga ccaggttttc atagctatcc
      841 ttgagtgtta ccgtaacttt tatcgcgccg cccgcggtgt aggccgtttt gtcggtaacg
      901 atggaggaat aagccaacac agctccgctt tcgcggatgg catacgcatc ggattgctgc
      961 gcgctactcc atgtcgatag tttcagcgtg gcgtaatggc gatcgccagc aatcttcgcg
     1021 gttcgggtgg tggtgtaaac gccatcagac tcttcgctcc agttacccgt ttcattttta
     1081 tctgtaccgc tcacctcgac cacactgcta cccgccagca gcgaagccat ccctttcacc
     1141 aggtttttct gctcatccat aagggtaact gacactttta tcttgccacc cgcggtgtat
     1201 gacacatcgt ccgtcttgat ggtggaggtc agctgcgaga tatcgccagt gataaagctc
     1261 acttccacag actgactact gccattgatc tcggccttga taagactgtt taccgcctgg
     1321 tcactggcaa ggttaacgct ggcaatcccg tctttatctg tcgtcacaat accgccattt
     1381 ggatcggcaa aaaccgccgc accattcacg accgtgaagg taacattttg tccctgcagt
     1441 aaattttgat gcacatccct gaccgtcaca gcgacggtat tgtgatcgcg tctggcaact
     1501 gcgttattcg cggtgatctg catcgcagcg attttggcgg tatctttatc acccgcaata
     1561 ctgaacgcat ccgaatgttt ggtttgccag ccgggcatcg tcagctgggc cgttaatttg
     1621 tcgccaatga gatgggccgt atagcttgct ttgtaaattc cctcactttc ttccgtccag
     1681 gcactaacgt catcagcctt cgtgttgtca actttaacgg tttgtttgag aagttctttt
     1741 cgtccggtaa ccccgttgcc gttatcatct ctcagggtca ctttaacgac tatcggctta
     1801 cccgcgacgt aattatcctg gtctgtctca atcgtggagt cagcgcggga tgccgtattc
     1861 gccacgatag caatcagcgc tggcgtctta gcgatatcgt cgccgttaaa ttgcggcatc
     1921 agcgataacg cgccagacgt cttcccggcg gtaactattt gggtataggt tccgttaccg
     1981 ttgtctttcc attcgcttaa ggcgaaatcc tgcaaacctt tcacctgtgt ttttaacgtc
     2041 atacctggga ttggcttgcc gctggaatct cgcgcggtat aggtcagcgt tgaggttgat
     2101 tttccatcag caagcaggat ttgctgatcg acagaaagcg ttgaatcggt gaggcttagt
     2161 gtgggttttt ccacgaccac catactttct tcacgccggg agaaatttcc tttcgaatct
     2221 tccgctgtaa ccgctatcgg gtaagtgttg tcggtttcag gcattgccgt gaatcggtag
     2281 ggaggaatag tgacctgaat atccttgccg gaaacagcta cttttccgcc cgctgattgc
     2341 agggaggcgg cttcaatgtc ataacctttc agcgcatatt tggtttgcaa ggaagaaacc
     2401 agcgatttaa cctcacccgg cttgcctttc aacggatcgg tcagcgtcag ccgcaccagc
     2461 tctttcttgc gatattcgag gacaatattg ttattacgat cgaccaggtc atagcgactg
     2521 cccacaaggc tgcgtcgtgc tgcaacttca gccggatcga gctgtttttg cagtgaatgg
     2581 ccgggttgca acgtcaactc cataccaatt cgggtgtcgt tttctccctg tttgccctgc
     2641 cgttgctcgg cactaaagga gatcagggga acgggggtgt aactcaaacc tgcggtaatc
     2701 gcgtgaggat cattttgtcg ctcgtcttta ccaaacagcg ccacttcatc gccataatat
     2761 tgttcataga ccagcttgcc gcccagctgc ggccaggccg gtaaccagcc ttcggcgcga
     2821 agatcccacc cgttcgccgg acgtgcttca tagtcattgt cgagttccgg tgcactacgc
     2881 cagttactga ggcgcagata accgttgccg ctcagtttca ggtaatcgcg ccagtattca
     2941 acgcccatcc cggttcgggt gtgatagcgg gtcaggtcat gatcgataaa catattgacg
     3001 ccagacatcc agctcgatgt gaaataacgc cagccgatac cgtggttggt ttgagtgcga
     3061 tcatccgtac ggtgaagtgt gtgctggctg aagaccaggt tatccggcgt ttcgtaccag
     3121 ggatggagaa attcaaatga cgagctttta aggctgaaat cttcatccac accaagagat
     3181 acccgggcag ttccccaacg actgagccag tccgttgcct gcgcagaggc agaagaagcc
     3241 acccagccac gcgccatact cgccgcctgt tcactgtcca tatcgcgtgc taaaagtgtt
     3301 ccgctttgct gcgccatttc cgcgacctgc atttgcggat ctttgccgtc gcgctccatc
     3361 tgcattgctt tcaggttgcg cgcttcaggg ctattactgt taatgagggg cacatctatt
     3421 tcatcgccct gacgcacatt gtcaaagccc cgcgcaaagg tacggaactg attcaggcga
     3481 cgtaattcat caacggtgat accaaagcgt gccgcgaccg ttggtggcga ttccaacgca
     3541 cccagggtat atggaatagt tttaacgttc ggcaggatca gtgacgcggc agtatccgag
     3601 tgcgcagaat taatatgcgt aggggcagca gccataacag ggaaaatagt ttgtgttatc
     3661 aggcaaatac cggcggtcag acgacgtaaa cgatccccca atccacatat ggtgttatcg
     3721 ccccgacgat cgccattccc atgcgctttc cctgccattt gtcatccctg tcgctatgcg
     3781 ttcagattaa atgacctttc tttaacatga gggaaaatga tgattcatga ttagcaaatg
     3841 atgaatacag gcggaataat ggaaataaca acaagataag aacaatacct gatcaagggt
     3901 tatcaggggg aagaacagga gagctttccc gccagagcag taacccctcc tgtaaatacg
     3961 ttacttcatt ttcacctttg cggtgaccaa taacgccgtc agcaacatca atgtgccgga
     4021 caacatcaac ggcgaagtca gaccaatatt atccagcgca taaccgccga ttgccgcgcc
     4081 acaggtatta gcaagctgaa taaccgccac ctgaatagac ccggcttttt ctgcctgatc
     4141 ggccagtgag cgggtgatcc acgttgacca gccgacagga accaatgcaa aagttagccc
     4201 ccagataatc gccacgccgg tagcaacgat tttatcgctt ccccacaacg tcagcaccaa
     4261 cgcactcaca gccagtatta acggcgcgcc tgctaaggcc agttttaccg aacgtttaag
     4321 aatgaacgac gaaagcgacg taccaataaa gctggcgata ccaaaactca acagcaccag
     4381 cgttaagcca tccacgccga atcccgccag gttcatatac accgggcgaa tatacgtgaa
     4441 gaaagcaaac tgcccggcga aagacatgaa tatggcgatc atccctgcca tcacacccgg
     4501 acgttgtaat aagcggaaag tattttgttt ctgatgcgag ggttcgcctg gcagcgaagg
     4561 caatgatttg ataatccaga aaatacacag cacgcccatc accgccgccg cattaaagac
     4621 attgcgccaa ccgataagct cgcctaaaaa actgcccaac ggtgcggcaa tcaccagcgc
     4681 aatagaaacc gcgccgaaga tcaccgacag cgccttcggc accgtacgcg gcggtaccag
     4741 acgcatggtc agcgacgccg acatcgccca gaacccaccc agcgccagcc ccagacaggc
     4801 acgaccgatt aaaagcaaac tgaatgagtt agcaaaggaa accagcaagc aggagagcgt
     4861 cagcaaaacg gcaaacaaaa taacaacata gcggcggtca gtggcctgaa tggtctgggt
     4921 aataaacaaa ctggcaaaca ttgccacaaa ggcggtcacg gtcaccgatt gcccggcaac
     4981 cccttccgaa atgcctaaat cctgggccat tggcgtcaac aaactgacgg gcaaaaactc
     5041 aacgataatc agacaggcga cgcaaaacgc caccgagaat acggctgacc agttcggtcg
     5101 ggtgatgaca tccgcgccgc ggttttcggc aataaattca ctcatggtgt tacccgtggc
     5161 atgtttcagg ggaaaagccg accactttaa cattgaaatg tgtgacgcat ttaacgtttt
     5221 tgcaactttt acggtgttgt gggaaaagtt cgaggctgct cgaagaaata aaaatcatag
     5281 ggcaaggtga tatgtatttt cgcgattgtg ttgggaacaa gggataaagt attgcacttg
     5341 gtgatatact ttaataacag gatctcaacg atatacgcgt gtttaaaacc aaatggtttg
     5401 caagggaagc caggtctcat gcaatgacag atgaagagtt gtgccgggcc attttagaaa
     5461 cggaacaagg taaagctgat gtacttggtg gtggcgtttt taaaaagcgc cttcaccaaa
     5521 atcgtgagcg tgcaattatt ctggcaaaag ggggaagcaa ttggttttac acgtttttat
     5581 atgcaaaaca ggatatgtcg aacatcaata gtcaggaact ggctgggttt cgtgaacttg
     5641 caaagcatta tgccttcctg acgaaagccc aactaacagc aatgattaac actaaagaac
     5701 tgacagagat ctgctatgac tgcaaaaact aaatttaaaa gtcctgcatt tgaggctatt
     5761 cacagcgcag cggctggatt gtccagcgtc gatgctattt ctgccgaaac aatgcgtact
     5821 tttgacaaag cctgcctgac cagcgtgcaa gacttacaac ctgttgagat aaaagcactg
     5881 cgggaaaaat taaaagtgag tcagcccgtc ttcgctcact atctcaatac cagcgtttca
     5941 actgtacaaa aatgggaaag tggcgctaaa cgccccaatg gcatttcatt aaaactgctc
     6001 agcattgtgc aaaaacacgg gctggaggtg ctgctgtaaa tagaacgtag ataatgcgtt
     6061 cacctactgt cagttccgat accggatgcg gcatgaacgc cttatccacc cgacaggtca
     6121 ggcgcggtct gtaggctgat aagacgaaac gattttaatg cagccagtga acgccttcgg
     6181 caggctgaaa gaacgcgtta tagcgcatct gaaaagcatt aatatcctgc atttcaggtt
     6241 cggtttcgcg cagaaaaccc agcgccagcc tgacctgagc atcggtaatc ggcgcggcaa
     6301 gacgggcttt ttgtaaaatc cgatgccatt gcagcaggtt ctgttcgcta ccatcgacaa
     6361 tgacaatttg ccagatgagc aacacctgcg cggcgttttg caggtgcgac tcatccgggc
     6421 gatgcaaata acgtaaaaat tcgtcaccgg ctgactttcc catttgatcg accatcgact
     6481 ccacgggtac gacatcgata ttcagccgat cgctgagacg gcgaatcgct tttctggccc
     6541 cggatgtcag cacccgaaaa ccaatcacaa acactaccgc cagcgtggcg agcattatcc
     6601 agatcatgag ttctcctgcg taatcagaac tcatgatatc tggaaaccct caccagttac
     6661 taatccacca gaataatctt cagtgcaaac agagctgcga ccaccaccac acacaggttc
     6721 agatcgcgcc agcgcccggt gcatactttc atgatgcagt acgacataaa gccgagtgca
     6781 atcccttcgg tgatcgagaa agtaaagggc atcatcaccg tggtaataaa cgcaggcacc
     6841 gattcggtaa aatcatccca gttaacgcgc gccaggctcg aagtcatcag cacaccaaca
     6901 aagattaacg ctccggcggt tgcgtaagga ggaaccatcg ccaccagcgg tgagaagaac
     6961 ataaccaaca ggaacataac gccgaccaca accgcagtca gccccgtgcg accaccgact
     7021 gccacaccag aagtactttc aatataggcg gtaacagacg aggtgccgat aaacgcgccc
     7081 gccaccgaac tgacgctatc aacatatagc gccttattca tattggggaa tttaccgtta
     7141 ctatctatta agccagcttt atcagttaca ccaattaatg ttcctgatga atcaaatagg
     7201 ttgatcagca tgaaggagaa aatgataccg gcgagttcaa gtgttaacgc gccgctcaaa
     7261 tctacttcac caatgacgcc gctaatatca ggcggaatgg aatagacgcc gctaaaatga
     7321 acatcaccga aaaataatcc acagcaagac gtcaccacaa tagaaacaag caccgcggca
     7381 tgaaaatgac gtgatgacaa cacggttata ataaaaaacc ctaaaatacc taacaacacg
     7441 ccgtgagaac ttaaatcgcc aatcatcacc agggtgtctt tattggcgac aataacgcca
     7501 gtatttttta atcccattaa ggcgataaat aatccgattc cgctggtgat accaatacgt
     7561 aaacttaatg gaatgttgga gatcatccag taccggatac gaaacagcgt gagcagaaat
     7621 agcccaactg cgccccagaa tatcgcgccc atcccggtct gccaggagat gcccatcgcc
     7681 cccacgacca cgaacgcaaa gaaggcgttc agccccattg ccggagccag cgccacgggt
     7741 aagttagcaa atat
//
LOCUS       DAMZFT010000198         7613 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.198, whole genome shotgun sequence.
ACCESSION   DAMZFT010000198 DAMZFT010000000
VERSION     DAMZFT010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7613)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7613)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7613
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.198"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..469
                     /gene="lptD"
                     /locus_tag="PN977_004719"
     CDS             <1..469
                     /gene="lptD"
                     /locus_tag="PN977_004719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LPS assembly protein LptD"
                     /protein_id="HDI6044654.1"
                     /translation="TRLDNVATSNSSIEYRRDEDRLVQLNYRYASPEYIQATLPKYYS
                     TAEQYKNGISQVGAVASWPIADRWSIVGAYYYDTNANKQADSMLGVQYSSCCYAIRVG
                     YERKLNGWDNDKQHAVYDNAIGFNIELRGLSSNYGLGTQEMLRSNILPYQNTL"
     gene            522..1808
                     /gene="surA"
                     /locus_tag="PN977_004720"
     CDS             522..1808
                     /gene="surA"
                     /locus_tag="PN977_004720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase SurA"
                     /protein_id="HDI6044655.1"
                     /translation="MKNWKTLLLGIAMIANTSFAAPQVVDKVAAVVNNGVVLESDVDG
                     LMQSVKLNAAQARQQLPDDATLRHQIMERLIMDQIILQMGQKMGVKISDEQLDQAIAN
                     IAKQNNMTLDQMRSRLAYDGLNYNTYRNQIRKEMIISEVRNNEVRRRITILPQEVESL
                     AQQVGNQNDASTELNLSHILIPLPENPTSDQVNEAESQARAIVDQARNGADFGKLAIA
                     HSADQQALNGGQMGWGRIQELPGIFAQALSTAKKGDIVGPIRSGVGFHILKVNDLRGE
                     SKNISVTEVHARHILLKPSPIMTDEQARVKLEQIAADIKSGKTTFAAAAKEFSQDPGS
                     ANQGGDLGWATPDIFDPAFRDALTRLNKGQMSAPVHSSFGWHLIELLDTRNVDKTDAA
                     QKDRAYRMLMNRKFSEEAASWMQEQRASAYVKILSN"
     gene            1808..2797
                     /gene="pdxA"
                     /locus_tag="PN977_004721"
     CDS             1808..2797
                     /gene="pdxA"
                     /locus_tag="PN977_004721"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HDI6044656.1"
                     /translation="MVKTQRVVITPGEPAGIGPDLIVQLAQREWPVELVVCADATLLT
                     DRAAMLGLPLTLRPYSPNSPAQPQTAGTLTLLPVALRESVTAGQLAVENGHYVVETLA
                     RACDGCLNGEFAALITGPVHKGVINDAGIPFTGHTEFFEERSQAKKVVMMLATEELRV
                     ALATTHLPLRDIADAITPALLHEVIAILHHDLRTKFGIAEPRILVCGLNPHAGEGGHM
                     GTEEIDTIIPVLDELRAQGMKLNGPLPADTLFQPKYLDNADAVLAMYHDQGLPVLKYQ
                     GFGRGVNITLGLPFIRTSVDHGTALELAGRGKADVGSFITALNLAIKMIVNTQ"
     gene            2794..3615
                     /gene="rsmA"
                     /locus_tag="PN977_004722"
     CDS             2794..3615
                     /gene="rsmA"
                     /locus_tag="PN977_004722"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HDI6044657.1"
                     /translation="MNNRVHQGHLARKRFGQNFLNDQFVIDSIVSAINPQKGQAMVEI
                     GPGLAALTEPVGERLDQLTVIELDRDLAARLQTHPFLGPKLTIYQQDAMTFNFGELAE
                     KMGQPLRVFGNLPYNISTPLMFHLFSYTDAIADMHFMLQKEVVNRLVAGPNSKAYGRL
                     SVMAQYYCNVIPVLEVPPSAFTPPPKVDSAVVRLVPHATMPHPVKDVRVLSRITTEAF
                     NQRRKTIRNSLGNLFSVEVLTGMGIDPAMRAENISVAQYCQMANYLAENAPLQES"
     gene            3618..3995
                     /gene="apaG"
                     /locus_tag="PN977_004723"
     CDS             3618..3995
                     /gene="apaG"
                     /locus_tag="PN977_004723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HDI6044658.1"
                     /translation="MINSPRVCIQVQSVYIEAQSSPDNERYVFAYTVTIRNLGRAPVQ
                     LLGRYWLITNGNGRETEVQGEGVVGVQPLIAPGEEYQYTSGAIIETPLGTMQGHYEMI
                     DENGVPFSIDIPVFRLAVPTLIH"
     gene            4002..4850
                     /gene="apaH"
                     /locus_tag="PN977_004724"
     CDS             4002..4850
                     /gene="apaH"
                     /locus_tag="PN977_004724"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     ApaH"
                     /protein_id="HDI6044659.1"
                     /translation="MATYLIGDVHGCYDELIALLHKVEFTPGKDTLWLTGDLVARGPG
                     SLDVLRYVKSLGDSVRLVLGNHDLHLLAVFAGISRNKPKDRLTPLLEAPDADELLNWL
                     RRQPLLQIDEEKKLVMAHAGITPQWDLQTAKECARDVEAVLSSDSYPFFLDAMYGDMP
                     NNWSPELRGLGRLRFITNAFTRMRFCFPNGQLDMYSKESPEEAPAPLKPWFAIPGPVA
                     EEYSIAFGHWASLEGKGTPEGIYALDTGCCWGGTLTCLRWEDKQYFVQPSNRHKDMGE
                     GEAAAS"
     gene            4875..5474
                     /locus_tag="PN977_004725"
     CDS             4875..5474
                     /locus_tag="PN977_004725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000796364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4291 domain-containing protein"
                     /protein_id="HDI6044660.1"
                     /translation="MADYFEIRADYNQHTITVYQAYNDAIADVAVRDGRFGAPFSFNR
                     MTWIKPSFMWMMERSNWGLKKDQQRILAIRIKRTFFDTLLEQAVLTTPEAHVYPHAGV
                     WETLFAQANVYVQWDPERSINGKKLEHRSLQLGISRNLISQFNEDAIVAIDDLTPLVR
                     KCHNLLINGKTTQAKSFLPPEKIYPVSATARKAWGMKNS"
     gene            5510..5959
                     /locus_tag="PN977_004726"
     CDS             5510..5959
                     /locus_tag="PN977_004726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001248983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6044661.1"
                     /translation="MRQINAGLNTREIILEMIDEADEPPFAEQRTWHRRRIAFLCECI
                     SVAVDYGFFCSVSQQSIMNITRMAEDVWITESKTTQLIDIWQQLKQLYPPETIPYTQQ
                     LPAKELLELLLSSPDDLRIIEIPDIIGDVFDDQTWIRLFKKHFADVI"
     gene            complement(6076..6255)
                     /locus_tag="PN977_004727"
     CDS             complement(6076..6255)
                     /locus_tag="PN977_004727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system CcdA family
                     antitoxin"
                     /protein_id="HDI6044662.1"
                     /translation="MNDKTSDFTSERDFVSRAHCVGLNPQATLQWTEVNEDAISALNE
                     LAEETGCFSDEYRKF"
     gene            complement(6276..6755)
                     /gene="folA"
                     /locus_tag="PN977_004728"
     CDS             complement(6276..6755)
                     /gene="folA"
                     /locus_tag="PN977_004728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 dihydrofolate reductase"
                     /protein_id="HDI6044663.1"
                     /translation="MISLIAALAVDRVIGMENAMPWNLPADLAWFKRNTLNKPVIMGR
                     HTWESIGRPLPGRKNIILSSQPGTDDRVTWVKSVDEAIAACGDVPEIMVIGGGRVYEQ
                     FLPKAQKLYLTHIDAEVEGDTHFPDYEPDDWESVFSEFHDADAQNSHSYCFEILERR"
     gene            complement(6947..>7613)
                     /locus_tag="PN977_004729"
     CDS             complement(6947..>7613)
                     /locus_tag="PN977_004729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HDI6044664.1"
                     /translation="PRVIIAGFGRFGQITGRLLLSSGVKMVVLDHDPDHIETLRKFGM
                     KVFYGDATRMDLLESAGAAKAEVLINAIDDPQTNLQLTEMVKEHFPHLQIIARARDVD
                     HYIRLRQAGVEKPERETFEGALKTGRLALESLGLGPYEARERADVFRRFNIQMVEEMA
                     MVENDTKARAAVYKRTSAMLSEIITEDREHLSLIQRHGWQGTEEGKHTGNMADEPETK
                     PSS"
BASE COUNT         1902 a         1981 c         1936 g         1794 t
ORIGIN      
        1 tacacgtctg gataacgtag cgaccagtaa ctccagcatt gaataccgtc gggatgaaga
       61 ccgtctggta cagctgaatt accgttacgc cagcccggaa tatattcagg ctacgctgcc
      121 taagtactat tccacagctg agcaatataa gaatggtatt tcacaggtag gtgccgtcgc
      181 cagctggcca attgccgatc gttggtcaat tgttggggcc tactattacg acaccaatgc
      241 gaacaagcaa gccgactcta tgttaggtgt gcaatacagc tcctgctgct atgcaattcg
      301 cgtcggttac gagcggaagc tgaacggttg ggataacgat aaacaacatg cggtatatga
      361 caacgcaatc ggctttaaca tcgaacttcg cggcctgagc tccaactacg gtctgggtac
      421 gcaagagatg ctgcgttcga acattctgcc gtatcaaaac actttgtgat ttgttgattt
      481 accacgtaat ccgcagtgcg gttaattgaa atggaaaaag tatgaagaac tggaaaacgc
      541 tgcttctcgg tatcgccatg atcgcgaata ccagtttcgc tgccccccag gtagtcgata
      601 aagtcgcagc cgtcgtcaat aacggcgtcg tgctggaaag cgacgttgat ggattaatgc
      661 agtcggtaaa actgaacgct gctcaggcaa ggcagcaact tcctgatgac gcgacgctgc
      721 gccaccaaat catggaacgt ttgatcatgg atcaaatcat cctgcagatg gggcagaaaa
      781 tgggagtgaa aatctccgat gagcagctgg atcaggcgat tgctaacatc gcgaaacaga
      841 acaacatgac gctggatcag atgcgcagcc gtctggctta cgatggtctg aactacaaca
      901 cctatcgtaa ccagatccgc aaagagatga ttatctctga agtgcgtaac aacgaggtgc
      961 gtcgtcgcat caccatcctg ccgcaggaag tcgaatccct ggcgcagcag gtgggtaacc
     1021 aaaacgacgc cagcactgag ctgaacctga gccacatcct gatcccgctg ccggaaaacc
     1081 cgacctctga tcaagtgaac gaagcggaaa gccaggcgcg tgccattgtc gatcaggcgc
     1141 gtaacggcgc tgatttcggt aagctggcga ttgctcattc tgccgaccag caggcgctga
     1201 acggcggcca gatgggctgg ggccgtattc aggagttgcc ggggatcttc gcccaggcat
     1261 taagcaccgc gaagaaaggc gacattgttg gcccgattcg ttccggcgtt ggcttccata
     1321 ttctgaaagt taacgacctg cgcggcgaaa gcaaaaatat ctcggtgacc gaagttcatg
     1381 ctcgccatat tctgctgaaa ccgtcgccga tcatgactga cgaacaggcc cgcgtgaaac
     1441 tggaacagat tgctgccgat atcaagagtg gtaaaacgac ttttgctgcc gcagcgaaag
     1501 agttctctca ggatccaggc tctgctaacc agggcggtga tctcggttgg gctacaccag
     1561 atattttcga tccggccttc cgtgacgccc tgacccgcct gaacaaaggt caaatgagtg
     1621 caccggttca ctcttcattc ggctggcatt taatcgaact actggatacc cgtaatgtcg
     1681 ataaaaccga cgcggcgcag aaagatcgtg cgtaccgcat gctgatgaac cgtaagttct
     1741 cggaagaagc agcaagctgg atgcaagaac aacgtgccag cgcctacgtt aaaatcctga
     1801 gcaactaatg gttaaaaccc aacgtgttgt gatcactccc ggcgagcccg ccgggattgg
     1861 cccggactta attgtccagc ttgcacagcg tgagtggccg gtcgaactgg ttgtttgtgc
     1921 cgatgccact ctccttaccg accgggcagc gatgctcggt ttgccgctca ccctccgccc
     1981 ttattccccc aactcccctg cacaaccgca aactgcgggc acattaacgc tacttcctgt
     2041 cgcgctacgt gaatctgtca ctgcggggca gttagcggtt gaaaatgggc attatgtggt
     2101 ggaaacgctg gcgcgagcgt gtgatggctg tctgaacggt gaatttgctg cgctgatcac
     2161 aggccccgtg cataaaggcg tcattaacga cgcaggcatt ccgtttaccg gtcataccga
     2221 gtttttcgaa gagcgttcgc aggcgaaaaa agtggtgatg atgctggcaa ccgaagaact
     2281 tcgcgtggcg ctggcaacga cgcatttacc gctgcgcgat atcgcagatg ctatcacccc
     2341 tgcgcttttg cacgaagtga ttgctatttt gcatcacgat ttgcggacca aatttggtat
     2401 tgccgaaccg cgcattctgg tctgcgggct gaatccgcac gcgggcgaag gcggtcatat
     2461 gggtacggaa gagatagaca ccattattcc ggtgctcgac gagctgcggg cgcaggggat
     2521 gaaactcaac gggccgctgc ctgccgatac cctgtttcag ccgaaatatc tcgataacgc
     2581 cgacgccgtg ctggcgatgt accacgatca gggtcttccc gtgctaaaat accagggctt
     2641 cgggcgcggt gtgaacatta cgctgggcct gccctttatt cgcacatcag tggaccacgg
     2701 caccgcgctt gaactggcgg gacgtggcaa agccgatgtc ggcagtttta ttacggcgct
     2761 taatctcgcc atcaaaatga ttgttaacac ccaatgaata atcgagtcca ccagggccac
     2821 ttagcccgta aacgcttcgg gcaaaacttt ctcaacgatc agttcgtgat cgacagcatt
     2881 gtttctgcca ttaacccgca gaagggccag gcgatggtcg aaatcggccc cggtctggcg
     2941 gcattgaccg aaccggtcgg cgaacgtctg gaccagctga cggtcatcga acttgaccgc
     3001 gatctggcgg cacgtctgca aacgcatcca ttcttaggcc cgaaactgac gatttatcag
     3061 caggatgcga tgacctttaa ctttggcgaa ctggccgaga aaatgggtca gccgctgcgt
     3121 gttttcggca acctgcctta taacatctcc acgccgttga tgttccatct gtttagctat
     3181 actgatgcca ttgccgacat gcactttatg ttgcaaaaag aggtggtgaa tcgtctggtt
     3241 gcaggaccga acagcaaggc gtatggtcga ttaagcgtca tggcgcaata ctattgcaac
     3301 gtgatcccgg tgctggaagt accgccgtca gcctttacac caccacccaa agtggattcc
     3361 gccgtcgtgc gcctggttcc tcatgcaacg atgcctcacc cggttaaaga tgttcgcgtg
     3421 ttgagtcgca tcaccaccga agcctttaac cagcgtcgta aaaccattcg taacagcctc
     3481 ggcaacctgt ttagcgtcga ggtgttaacc ggaatgggga tcgacccggc gatgcgagcg
     3541 gaaaatatct ctgtcgcgca atattgccag atggcgaact atctggcgga gaacgcgcct
     3601 ttgcaggaga gttaacgatg atcaattcgc cccgagtgtg tattcaggtt caaagcgtct
     3661 acattgaggc acaatcttca cctgataatg aacgttacgt ttttgcttat accgtaacca
     3721 tacgcaatct ggggcgagcg ccagtgcagt tgttggggcg ttactggctg atcaccaatg
     3781 gcaatggccg tgaaaccgaa gtccagggcg aaggagtggt tggcgtccag ccacttatcg
     3841 cgcctggcga agagtaccag tacaccagcg gtgcaatcat tgaaaccccg ctgggcacca
     3901 tgcagggtca ctacgaaatg atcgatgaaa atggcgtccc tttcagcatc gacattcccg
     3961 tattccgact cgccgttccc acactcattc attaaaagaa tatggcgaca taccttattg
     4021 gcgacgttca tggttgttac gatgaactga tcgcattgct gcataaagta gaatttaccc
     4081 ctgggaaaga taccctctgg ctgacgggcg atctggtcgc gcgcggcccg ggttcgctgg
     4141 atgttctgcg ctatgtgaaa tccttaggcg acagcgtacg tctggtgctg ggcaatcacg
     4201 atctgcatct gctggcggta tttgccggga tcagccgcaa taaaccgaaa gatcgcctga
     4261 caccgctgct ggaagcgccg gatgccgacg agctgcttaa ctggctgcgt cgccagcctt
     4321 tgctgcaaat cgacgaagag aaaaagctgg tgatggccca cgccgggatc acgccgcagt
     4381 gggatctgca gaccgccaaa gagtgcgcgc gcgatgtaga agcggtgctg tcgagtgact
     4441 cctatccctt tttccttgat gccatgtacg gcgatatgcc aaataactgg tcaccggaat
     4501 tgcgggggct gggaagactg cggtttatca ccaacgcctt tacccggatg cgtttttgct
     4561 tcccgaacgg tcaactggat atgtacagca aagaatcgcc ggaagaggcc cctgccccac
     4621 tgaaaccgtg gtttgcgatt cctggccccg tcgctgaaga atacagcatc gcctttggtc
     4681 actgggcatc gctggagggc aaaggtacgc cggaaggtat ttacgcgctg gataccggct
     4741 gctgttgggg cgggacatta acctgcctgc gctgggaaga taaacagtat tttgtccagc
     4801 cgtcgaaccg gcataaagat atgggtgagg gagaggcggc cgcgtcttaa cgctttacca
     4861 ttcagggagg aataatggct gactattttg aaatacgtgc tgattacaat cagcacacca
     4921 tcacggttta tcaggcttac aatgatgcaa tcgctgacgt cgccgtacgt gacgggcgat
     4981 ttggcgcacc tttttcattc aaccgcatga cctggattaa accctctttt atgtggatga
     5041 tggagcgtag caactgggga ctgaaaaaag atcaacaacg cattctcgca atccgaatca
     5101 aacgcacctt ctttgataca ttgctggaac aagcggtgct aaccacgccg gaagcccatg
     5161 tctatccaca tgcaggagtt tgggaaacgt tatttgcgca agcaaatgta tatgttcagt
     5221 gggacccaga acgcagcata aatggcaaaa aactggagca ccgatcatta cagcttggta
     5281 tttctcgtaa cttgatcagt caatttaacg aagacgccat tgttgccatt gatgatctga
     5341 cgccgctggt gcgaaagtgt cataaccttt tgataaacgg taaaacaaca caggcgaaat
     5401 ccttcctgcc gccagaaaaa atttatcctg ttagtgcaac tgcaagaaag gcttggggaa
     5461 tgaaaaattc ctgagacaaa tacgcgccta ttaagcggaa aatatctaag tgcgacaaat
     5521 caacgccggg cttaatacca gagaaattat tctggaaatg attgacgaag cagatgaacc
     5581 cccgtttgct gagcagcgaa cctggcatcg caggcgaatc gcttttcttt gtgagtgcat
     5641 cagcgttgct gtcgactacg gcttcttctg ttctgtctct cagcagtcaa taatgaacat
     5701 aaccaggatg gctgaagatg tatggataac agaaagtaaa acgacccaac tcattgatat
     5761 ctggcagcaa cttaagcagc tttatccgcc agaaactatt ccctataccc agcagttacc
     5821 cgctaaagaa ttgcttgaac tgctactttc cagtcctgac gatcttcgaa taattgaaat
     5881 ccctgacatc atcggcgatg tttttgatga ccaaacctgg attcgattat tcaaaaaaca
     5941 ctttgctgat gttatctaac tacgatgtga tgtgtaacaa tctaaaatac atcgccagac
     6001 gacagaccaa aaagaaagta tttagcagat actcaacatt ctgacttctg tacagaaata
     6061 gcagagttta aaaacttaaa atttccggta ctcatcagag aaacatcctg tttcttctgc
     6121 aagttcattt aatgccgaga tggcatcctc gtttacttca gtccactgta gagtggcttg
     6181 agggtttaga cctacgcaat gagcgcgact cacaaaatcg cgctcactgg taaaatcaga
     6241 cgtcttatcg ttcaatttgt agtcatagca aaaacttacc gccgctccag aatctcaaag
     6301 caatagctgt gagagttctg cgcatcagcg tcgtggaatt cgctgaatac cgattcccag
     6361 tcatccggct cgtaatccgg gaaatgggtg tcgccttcca cttctgcgtc aatatgcgtc
     6421 agatacagtt tttgcgcttt tggcaggaac tgctcataaa cgcgaccacc gccaatcacc
     6481 atgatttctg gtacatcacc acacgccgcg atggcttcat caaccgattt cacccacgtt
     6541 acacgatcgt ccgtacccgg ttgactgctg aggataatat ttttgcgtcc tggcagcgga
     6601 cgaccgatgg attcccaggt atggcggccc ataattacgg gtttatttaa ggtgttgcgt
     6661 ttaaaccagg cgagatcggc aggcaggttc cacggcatgg cgttttccat gccgataacg
     6721 cgatctaccg ctaacgccgc aatcagactg atcattgaga tttcccgata aaaaaaattg
     6781 tcgccactat acgtaaagcg taaaccgtcg tcgactggtg cgaggatgat gttgaggaaa
     6841 attttatatt ctgctggcga gtccacgctc tcccactgga ctcgccgcat tacaatgaaa
     6901 taaaaacaat tagttagctg taaagtgtga tttacgtcac tctttattag gatgagggtt
     6961 tcgtttccgg ttcatccgcc atattgccag tatgtttccc ttcttcggtt ccctgccagc
     7021 catgtcgttg aattaatgac agatgttcac ggtcttcggt aatgatctca cttaacatcg
     7081 cgctggtgcg tttatagacc gccgcacggg ctttggtgtc gttctcaacc attgccatct
     7141 cttccaccat ctgaatatta aagcggcgga acacatcggc acgttctcgc gcttcatacg
     7201 gccccagacc taaactttcc agtgccagac gcccggtttt gagcgcacct tcgaaggttt
     7261 cacgctccgg cttttcaacg cctgcctgac gcaaacgaat gtagtggtcg acatcgcggg
     7321 cgcgggcaat aatctgcaaa tgcgggaaat gttctttcac catctctgtc agttgcaggt
     7381 tggtttgcgg atcgtcgatg gcgttaatca gcacttccgc tttcgccgct ccggcagatt
     7441 ccagtaaatc catccgcgtg gcatcgccat aaaacacttt cataccaaat ttacgcaagg
     7501 tttcgatatg gtccggatcg tgatcgagca ccaccatttt caccccgctg gagagcagta
     7561 aacgtccggt tatctgccca aaacgaccga atccggcgat gattacccgc ggc
//
LOCUS       DAMZFT010000199         7609 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.199, whole genome shotgun sequence.
ACCESSION   DAMZFT010000199 DAMZFT010000000
VERSION     DAMZFT010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7609)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7609)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7609
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.199"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(343..2361)
                     /locus_tag="PN977_004730"
     CDS             complement(343..2361)
                     /locus_tag="PN977_004730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001696682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HDI6044665.1"
                     /translation="MRLFLCEKPSQAGDIARVLGAARRVDGAIEGKDFVVTWAFGHLL
                     EQAPPEAYGEQFGSPWKIDVLPVFPEQWQMVVKDNVGKQFNVISRLLRKADEVVIATD
                     ADREGEIIARELLDYCGYTGVVRRLWLSALDESSIREALGRILPGEETEKLYEAGKGR
                     SRADWLTGINLTRLYTLKARECGVSGVLSVGRVQTPTLAMVVRRDAEIKNFIPVPYWQ
                     LYLWLEKDNVRFRTSWIAAAQYCDTEKRCVNVQAATAVLSQCRQAGVAVVRDVSSRRE
                     KKQPPLCFSLGTLQEVCSRKFGLGAQAVLDIAQSLYEKHKATTYPRTDCGYLPVSMYK
                     EVPDVLAALEKTDPDIAPELAKLSPDIVSGIWNDRKITAHHAIIPTRQPFNIGALSDN
                     ELKVYRLIRQHYLAQFLPVQELDVTEATFLVAGQLFGTRGRVEVVRGWRALFVSDSAG
                     EEEDVADDEQAMPLPVLAKGDECRPAEAELKEMQTTPPRHYTEGTLIAAMKNAAGMVS
                     DPALKKVLRENAGLGTEATRAEILETLFTRKYIEKQGKHIRATQLGAELIAGLPEALT
                     SPGTTALWEQALDDIAQGKLSLSSFMEKQKQWLNHLVAGARQQQLQITAPVTPACPLC
                     GSRMRKVKMKSGKGEFWSCQRYPECKGVISEQKPTSGKRGGRKIKKHG"
     gene            complement(2378..3103)
                     /locus_tag="PN977_004731"
     CDS             complement(2378..3103)
                     /locus_tag="PN977_004731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010615557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03761 family integrating conjugative element
                     protein"
                     /protein_id="HDI6044666.1"
                     /translation="MSEEQMKEPASRRVGALTSPVNIELHSRYAIMLWEGRRGTKSKS
                     AEDRRAAKIMSMPEAIKMTGGLYRDSLADNPYADETMWKVENAIVEATDKINAEVRVL
                     EGILRNIPSVITVRDALSRSPVDIGVFSRSPLGYRCVWLLVGYDQLALKTFQAAHLGL
                     ISRNRRDELLSSGAHLIRKIYGILQGYRTMAVTRDDIENKTEKAEKAIARYGMPHDDI
                     LSGKLRSSFSPPLNRKSRDASRQ"
     gene            complement(3205..3783)
                     /locus_tag="PN977_004732"
     CDS             complement(3205..3783)
                     /locus_tag="PN977_004732"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6044667.1"
                     /translation="MVKNRTVLAVPTVVKNCAVLAVSTVVKNCAVLAVPTVIKKCTVL
                     AVPTVVKNCAVLAVPTAVKKCTALAVPTVIEKCTVLAVPTVIKKCTVLAVPTVIKKCT
                     VLAVPTVVKNCAVLAVPTVVKNCAVLAVPTVIKKCTVLAVPTAVKKCTALAVATAIKK
                     CTVLPVATVVKKCPALPVATVFENCTELALPT"
     gene            complement(3783..5048)
                     /locus_tag="PN977_004733"
     CDS             complement(3783..5048)
                     /locus_tag="PN977_004733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006859635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDI6044668.1"
                     /translation="MALPAESLIAHTLRKMKASMAEKASREDGRIRSGLLFTGNVHDS
                     YPRRLLLDTRLSPLDKTAWMMIRLHALNNEGAVFPTYDELQLQLASPGCGKASRETVS
                     RVLTMLRLTGWLSLCKRVRDEKGRVRGNIYALHDEPLTCCDAEKFDPRYLDMVLDTVS
                     RSRNKTIRHTASEVLSDIKNEPGMRHYHSHITLFESRMGAAQTPGQLAARQRVNPMVN
                     ENTGRESGSDSVHTPAPVVQVTELSAPLTELSEKQEMSPLSSDSVPPEKSVSYHSVRK
                     PNNYVRNITHSVIKNTYVLPESVKSQISTEDTDMLTKQLQALPEELAGSVLSSLSQML
                     SKNTLQNPVGWMLAVMKRAREGSFNTPDMSVHESRSPARAPYQPVYNRDERERRRRKV
                     ASPETVSNIVAEFRASLKRANAARLAARV"
     gene            complement(5309..5530)
                     /locus_tag="PN977_004734"
     CDS             complement(5309..5530)
                     /locus_tag="PN977_004734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000769122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6044669.1"
                     /translation="MKLKMQAMDGPVIIESSDVTQFYPDHESGGELTAVEYLADGGRI
                     TARVRHSFYQVAAALAGAWRADDASKSGG"
     gene            complement(5527..5742)
                     /locus_tag="PN977_004735"
     CDS             complement(5527..5742)
                     /locus_tag="PN977_004735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001682047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6044670.1"
                     /translation="MRKISGMLNLEKARMQLRVLLSGPVPAITPMSHAVAIKVVPDAP
                     DASERRVAELEQACAAAGIRIKGEQVK"
     gene            complement(5791..6204)
                     /locus_tag="PN977_004736"
     CDS             complement(5791..6204)
                     /locus_tag="PN977_004736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006859639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6044671.1"
                     /translation="MTTITKERLLTIKQWRETYGPGSNVVLPAEEAEELARIVLASLE
                     AEPVAWMCDDEDGREYNGHNEFSYGRRGIPLYAVPPAPNGLVKAVAFYEQVKRENPPV
                     ETGAWKDAVDWVLKQACQAVNTGINERRTIEENDD"
     gene            complement(6215..6412)
                     /locus_tag="PN977_004737"
     CDS             complement(6215..6412)
                     /locus_tag="PN977_004737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016234701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6044672.1"
                     /translation="MDKNTTAYWNLSLDTECPKCGHNFDLLCDPDFWEFSGAKQACEE
                     IKGYETCCPECNHEFKTDFVY"
     gene            complement(6414..6635)
                     /locus_tag="PN977_004738"
     CDS             complement(6414..6635)
                     /locus_tag="PN977_004738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000013838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6044673.1"
                     /translation="MANSLLETCNNWQIQRAEILSRNPDMAMTIDNLDTLIERTVRSA
                     IDIAHRVDWDFREAERLAKEQEKAAGKGD"
     gene            complement(6637..7035)
                     /locus_tag="PN977_004739"
     CDS             complement(6637..7035)
                     /locus_tag="PN977_004739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001696190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6044674.1"
                     /translation="MITITKERLLTIQQWRETYGPGSNVVLPAEEAEELARIALALLE
                     AEPLVPVMYKGMKLLTKEGLELIRDGIAEATGLEAMCMAEALLSGASVPSVPEEMYWQ
                     DAPVGGSSKAAAYATGWNACRAAMLQGKGK"
     gene            complement(7369..7547)
                     /locus_tag="PN977_004740"
                     /pseudo
     CDS             complement(7369..7547)
                     /locus_tag="PN977_004740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_305741.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         1790 a         2237 c         1601 g         1981 t
ORIGIN      
        1 accaccggga gattggttgg ctgcaaagca gctatttagc atgctcattt ttaaaacagc
       61 cataaataaa gtcaagcaga aaacaccaat aaaaaacggc gctgaaaatt cagcgccgtc
      121 acgggttacc agccctgagg ttatctcact tttctccggg gatcactccc gacgcggttt
      181 tcacggagtt cgtgcgcctt gcaacacaac cggacgcagt ggtcgttgct gtatcagaaa
      241 cagagcaacg ccgttgttta atcgccactc tgtttcccgc tgataacggg ggtatacatc
      301 aaagcgtatg tactttaaca aataaaaaaa agcatgtaat catcatccat gctttttaat
      361 ttttctgccg ccccgtttcc cggatgtcgg tttctgctct gaaatcactc ccttacactc
      421 cggataacgc tggcagctcc agaattcccc tttcccgctt ttcatcttca ctttccgcat
      481 tctgctccca caaagagggc atgccggcgt aaccggtgcc gtgatctgca gttgctgctg
      541 ccttgcaccg gccaccaggt ggttaagcca ctgtttctgc ttttccataa atgacgacag
      601 tgacagttta ccctgtgcaa tatcatccag agcctgttcc cacagcgccg ttgtccccgg
      661 actggtcagc gcctccggca atcccgctat cagctccgcc cccagttgcg tggcacggat
      721 atgcttaccc tgcttctcga tatatttgcg ggtgaacaac gtttccagta tttctgcgcg
      781 ggtcgcttca gtgcccagcc ccgcattttc gcgtaacact tttttcagtg ccggatcact
      841 caccatcccc gcagcgtttt tcatcgccgc aatcagcgtg ccttccgtat aatgacgcgg
      901 tggcgtggtc tgcatctctt tgagttcagc ttcagcagga cgacattcat cgccttttgc
      961 cagtacggga aggggcatcg cctgctcatc gtctgcaaca tcctcttcct ctcccgccga
     1021 atctgacaca aaaagcgcac gccatcccct gacaacctcc actcgccccc gcgttccaaa
     1081 caactgcccc gcaacaagaa aagtggcctc tgtgacatcc agctcctgaa cgggtaaaaa
     1141 ctgagccaga taatgctgac gaattaaccg gtacacctta agctcattat cactcagcgc
     1201 cccaatatta aacggctgac gggtggggat tatcgcatga tgcgcggtga tcttcctgtc
     1261 gttccagatc cccgacacaa tatcaggact gagttttgcc agctccgggg caatatccgg
     1321 atcggttttt tccagcgctg ccagtacgtc aggcacttct ttgtacatcg acacaggcag
     1381 ataaccgcaa tctgtacgcg gatatgttgt cgccttatgc ttttcgtaca gcgactgggc
     1441 tatatcaaga acggcctgcg cgccgaggcc aaatttgcgc gaacagacct cctgcagcgt
     1501 tccgagcgaa aaacacagcg gaggctgctt tttctcccgt ctgctgctga catccctgac
     1561 aacagcaaca ccggcctgac gacactgtga caggacggct gttgccgcct gcacattcac
     1621 acaccgtttt tcagtatcgc agtactgcgc tgccgcaatc caggaggtac gaaaacgcac
     1681 attgtccttc tccagccaga gatacagttg ccagtaaggt accggaataa aatttttgat
     1741 ttctgcatcc cgtctgacca ccatcgcaag cgtgggcgtc tgcaccctgc caacagagag
     1801 gacgccggac acaccacact ccctggcttt cagggtatac agacgcgtca ggttgatccc
     1861 cgtcagccag tcagcccggc tgcgtccctt accggcttca tataacttct cggtctcctc
     1921 tccgggcagt atcctgccca gcgcctccct gatactgctc tcatccagtg cggacagcca
     1981 gagacggcgc acaacgccgg tataaccaca ataatccagc agttcccttg cgataatttc
     2041 gccttcacga tcggcgtcag tggcaatcac cacttcatca gccttacgga gaaggcggct
     2101 gatcacatta aactgcttcc cgacattatc cttcaccacc atctgccact gttcaggaaa
     2161 gaccggcaaa acgtcaattt tccagggact gccaaactgc tcaccatacg cctccggtgg
     2221 cgcctgctcc agcaagtgac caaaggccca ggtcacaaca aagtccttac cttcaatcgc
     2281 accatcaaca cgtctggcag ccccaagtac tctggctata tcaccggcct gtgaaggttt
     2341 ctcacacaaa aagagacgca tgaattctcc tcacctgtca ttgccgggat gcatcacggg
     2401 acttacggtt caggggcgga gaaaatgaag atctgagctt accggataga atatcatcat
     2461 gaggcatacc ataacgggca atagctttct ctgccttttc tgttttattc tcaatatcat
     2521 cccgagttac cgccatagtg cgatacccct gcagaatacc gtaaattttg cggataagat
     2581 gcgcaccaga agacaacaac tcatcacgcc tgtttcgtga aataagcccc agatgtgctg
     2641 cctgaaacgt ttttaatgca agctgatcat acccgacaag aagccataca cagcggtagc
     2701 ccagaggaga gcggctgaaa accccaatat cgacaggaga acgagaaaga gcatcccgga
     2761 cagtgatcac tgacggaata tttctgagga tcccttcaag tacacgaact tcagcattaa
     2821 ttttatcggt tgcctcaaca atcgcatttt caactttcca catcgtttca tccgcatagg
     2881 gattatcagc aagagaatct ctgtagaggc cgccagtcat cttaatagct tccggcatac
     2941 tcattatttt cgctgccctg cgatcttcag cacttttact tttcgtgcct cgtctccctt
     3001 cccacagcat gattgcataa cgggagtgca actcgatatt cactggtgaa gtaagcgcac
     3061 ctacacgacg ggatgcgggt tccttcattt gttcttcaga catgttatat ctccggcaaa
     3121 agaaaaggat atcgggctga aatagtcagc ctgcagacgg agatctgaca atcaaaaata
     3181 aaaaaaggaa tgactgaaat tacgtcatgt tggcagtgcc aactcagtac aattttcaaa
     3241 cactgttgcc actggcaacg ccggacactt tttaaccact gttgccactg gcaacaccgt
     3301 acatttttta atcgctgttg ctactgccaa cgccgtacac tttttaaccg ctgttggcac
     3361 tgccaacacc gtacattttt taatcactgt tggcactgcc aacaccgcac aatttttaac
     3421 cactgttggc actgccaaca ccgcgcaatt tttaaccact gttggcactg ccaacaccgt
     3481 acatttttta atcaccgttg gcactgccaa caccgtacat tttttaatca ctgttggcac
     3541 tgccaacacc gtacattttt caatcactgt tggcactgcc aacgccgtac actttttaac
     3601 cgctgttggc actgccaaca ccgcgcaatt tttaaccact gttggcaccg ccaacaccgt
     3661 acatttttta atcactgttg gcactgccaa caccgcacaa tttttaacca ctgttgacac
     3721 tgccaacacc gcgcaatttt taaccactgt tggcactgcc aacaccgtac gatttttaac
     3781 catcaaacac gcgccgccag acgggcagca tttgcacgtt tcaggctggc acggaattct
     3841 gcgacaatgt tgctcaccgt ttcaggtgac gccactttcc tgcgacgacg ttcacgttca
     3901 tcacggttgt acacaggctg atatggtgca cgggctggcg accgggattc atgcacggac
     3961 atatccggcg tattaaaact tccctcacgc gcacgtttca tcacagccag catccagcca
     4021 accggattct ggagcgtgtt tttgctgagc atctgtgaca ggctgctcag cacactacct
     4081 gccagttctt ccggcaacgc ctgcagttgt tttgtcagca tgtccgtgtc ttctgtgctg
     4141 atttgcgatt tcaccgactc aggcaatacg tacgtatttt taatcacact gtgtgtgata
     4201 ttacgtacgt aattgttcgg tttccgaacc gagtgataag atactgattt ttcaggcggt
     4261 acggaatccg aactgagtgg gctcatttcc tgtttttcac tgagttcggt taacggagca
     4321 ctgagttcgg ttacctgcac gacaggtgca ggggtatgta cggaatccga accactctct
     4381 ctgcctgtgt tttcattcac catcgggttc acccgctgac gcgccgccag ctgaccaggt
     4441 gtctgtgctg cccccatacg cgattcaaac agagtgatat ggctgtggta atgccgcata
     4501 ccgggctcat ttttgatatc actgagcact tcactggctg tgtgccggat tgtcttgttc
     4561 cggctcctgc tcacagtatc cagaaccata tccaggtatc gtgggtcaaa cttttctgca
     4621 tcacagcagg tcagtggctc atcatgcagg gcgtaaatat tcccccggac ccgccctttt
     4681 tcatccctga cccgcttaca cagactcagc cagcccgtaa gccgcagcat tgtcagcacc
     4741 cggctcactg tttcacggga cgccttcccg cacccgggag atgccagttg taactgcagt
     4801 tcatcgtacg tcggaaatac tgcgccctca ttattcaggg catgcagacg aatcatcatc
     4861 cacgcagttt tatcaagggg agaaaggcgc gtatccagca acagcctgcg gggatacgaa
     4921 tcgtgaacat tgccggtaaa caacagccca ctgcgtatcc tcccgtcctc acgactggct
     4981 ttctctgcca tgctggcctt catttttctg agcgtatgcg caataaggct ttctgcaggt
     5041 agcgccataa ataaactcct taaatcccgg cagactgcgc cggggaacgg tttccgaaat
     5101 gcttataact gaaccctgtc aaagatgcag gctaaacact tcaccacacg agcaccaccg
     5161 gactgactgt cgcgtccggc ttcagaagat attttcctta actgttgata cgctgacgct
     5221 cttcgtttgc cggctgcttc accgcaacat aaaaaattta tgtcgcggtt cagcagcttc
     5281 ggaacagaca atcattccgg cacagtcctc acccaccgct ttttgatgca tcatccgcac
     5341 gccatgcccc ggcaagtgcc gcagcaacct gataaaaaga gtgcctcacc ctcgcggtga
     5401 ttcttccccc gtcagcaagg tactcaacag cggtgagttc cccgccactt tcatggtcag
     5461 ggtaaaactg tgtcacatca ctactctcta taatcaccgg gccgtccatt gcctgcatct
     5521 tcagtttcat ttcacttgtt cccctttgat acgaatgccc gcggcagcgc aggcctgttc
     5581 cagttcagcc accctccgct cactcgcatc cggagcatca ggcacaactt taattgccac
     5641 ggcatgcgac atcggagtga tcgcaggaac cggtcctgat aacaaaacac gcaactgcat
     5701 cctggccttc tccaggttca acattccact gatttttctc atgaccggcg ctcctccagt
     5761 tctgcaatgc gcttttttgc tgcttccggc tcaatcatca ttttcttcaa ttgttcttct
     5821 ttcattgatg ccagtgttta cagcctgaca agcttgttta agcacccagt caacggcgtc
     5881 tttccatgct cccgtttcaa ctggcggatt ttcacgttta acctgctcat agaaagctac
     5941 ggctttaacc aatccattcg gcgctggcgg gacggcgtaa aggggaatcc cgcgtcttcc
     6001 ataagaaaac tcgttatgac cgttatattc acgaccatct tcgtcatcac acatccacgc
     6061 caccggttcc gcttccagcg atgccagtac aatccgtgcc agttcttccg cttcttctgc
     6121 tggcagtaca acgttgctac ccggtccgta tgtttcgcgc cactgcttga ttgtcagcag
     6181 tcgctctttg gttatagtgg tcatttgtta tgcctcaata cacaaaatct gttttaaatt
     6241 catggttaca ttctggacag catgtttcgt aaccttttat ttcttcacat gcctgttttg
     6301 ctccagaaaa ttcccagaaa tctggatcac aaagcagatc gaaattgtga ccgcattttg
     6361 ggcattcggt atcaagtgac agattccagt aagcagtggt gtttttatcc atatcagtct
     6421 cctttacccg ctgctttctc ctgctcttta gcaagccgct ccgcttctct gaaatcccaa
     6481 tccactcgat gtgctatatc aattgcagaa cgcacggtcc gttcaattag cgtgtccaga
     6541 ttatcaattg tcattgccat atctggattg cgagataaaa tctccgccct ctgaatctgc
     6601 cagttgttgc aggtttcaag taacgagtta gccatatcac tttccttttc cctgaagcat
     6661 tgcggcgcga caggcattcc agcctgtcgc gtatgcagct gctttgctac tgcctccaac
     6721 aggcgcatcc tgccaataca tttcttctgg cactgacggc actgacgccc cagacagcaa
     6781 agcctcagcc atacacattg cttccagtcc tgtagcctca gcaataccgt cacgaataag
     6841 ctcaagcccc tcttttgtta acagtttcat tcctttatac atgacgggca ccagcggttc
     6901 tgcttccagc aatgccagtg caattcgtgc cagttcttcc gcttcttctg ctggcaatac
     6961 aacgttgcta ccaggtccgt atgtttcgcg ccactgctgg attgtcagca gtcgctcttt
     7021 ggtaatagtg atcatgctgc gtttccttct ttcttattaa caattacacc gtcatatatt
     7081 tcattaaggt gccctctcaa ctccatgcgc cttaatgcag ataacatgta atcgcattca
     7141 acctgcttat ttccagtaaa tggcttatcg tcaggattac cccaacagca attacccctg
     7201 ggccacccat gtactttccg tactcttccg ttaacaacgt gaagtaatcc ccagccaggt
     7261 ggtaaatcct caattgaaat aattcccggc tcactaataa agaatcgcca gtcgcccatt
     7321 ccaagagatg gatttttacg aaaacgcttt tttctatctg ccaacaagtc agcacgagaa
     7381 catttcgcct ctatcaggca tgatgctgaa tttctgaatc ccatagcatc tggctgttct
     7441 ccggtactgg ttacagctat aaagcggtca tgaaaacaaa ccttgaaccc gttgcgctta
     7501 aggaacttgt acgcaatctg acagagttcg cggtgtgtta acgccatatc actctccttt
     7561 ggtgcgaatg ccagcgacgc gtggcacatt aacttccacg atgcgcaca
//
LOCUS       DAMZFT010000200         7606 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.200, whole genome shotgun sequence.
ACCESSION   DAMZFT010000200 DAMZFT010000000
VERSION     DAMZFT010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7606)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7606)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7606
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.200"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(216..763)
                     /locus_tag="PN977_004741"
                     /pseudo
     CDS             complement(216..763)
                     /locus_tag="PN977_004741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220840.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
     gene            900..1196
                     /locus_tag="PN977_004742"
     CDS             900..1196
                     /locus_tag="PN977_004742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001689019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HDI6044675.1"
                     /translation="MFDDGQATMWLSVLAFGRQAEALARHQKGELVSVAGNMQVSQWT
                     GQNGKTRQGRQVIADSVINTQTVRPGGSRRETTGTQGNQPPTGGDATYGDDIPL"
     gene            1214..1534
                     /locus_tag="PN977_004743"
     CDS             1214..1534
                     /locus_tag="PN977_004743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001066120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
                     /protein_id="HDI6044676.1"
                     /translation="MNNSTLKSRTISDATAEELESRGLWRRAARRWQDVMITLEKDSH
                     RQLAVMRARECIRKAKRPIPESRIDIHTVRKAADRTIQKMGLPSLTDEIWRDYPDSVN
                     DDGY"
     gene            1610..1786
                     /locus_tag="PN977_004744"
     CDS             1610..1786
                     /locus_tag="PN977_004744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein convertase"
                     /protein_id="HDI6044677.1"
                     /translation="MAGGVRYDLKDHPCGKVTQEHLANAGKRLDEIKKKQQERRKSNR
                     LIKTSFASLSCFLC"
     gene            1818..2066
                     /locus_tag="PN977_004745"
     CDS             1818..2066
                     /locus_tag="PN977_004745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence hypothetical
                     protein"
                     /protein_id="HDI6044678.1"
                     /translation="MNARKAVLADNPELILRLLQLKFDETLSYPRISAQTGVSKTAIF
                     SLVRRFHQVFTDGHLSGEYSCVQLTGLFSRGDNIQPRQ"
     gene            2063..2422
                     /locus_tag="PN977_004746"
                     /pseudo
     CDS             2063..2422
                     /locus_tag="PN977_004746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000422669.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(2364..2871)
                     /locus_tag="PN977_004747"
                     /pseudo
     CDS             complement(2364..2871)
                     /locus_tag="PN977_004747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312326.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
     gene            complement(3490..4071)
                     /locus_tag="PN977_004748"
                     /pseudo
     CDS             complement(3490..4071)
                     /locus_tag="PN977_004748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004237799.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
     gene            complement(4159..4341)
                     /locus_tag="PN977_004749"
     CDS             complement(4159..4341)
                     /locus_tag="PN977_004749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001354049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron(III) transporter permease"
                     /protein_id="HDI6044679.1"
                     /translation="MSSALLVFTDAMKELPVTLLYAEAARGTFEEGAISALMIVLAGT
                     FPVILFVPAERLKSED"
     gene            complement(4338..4517)
                     /locus_tag="PN977_004750"
     CDS             complement(4338..4517)
                     /locus_tag="PN977_004750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001470087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDI6044680.1"
                     /translation="MSVGVLLVICCTMRFQAIAIGALDVRLGRIPESLEQASRLLGEN
                     GTGTFVRVHFPVLLW"
     gene            complement(4862..6841)
                     /locus_tag="PN977_004751"
     CDS             complement(4862..6841)
                     /locus_tag="PN977_004751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007752107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoX family phosphatase"
                     /protein_id="HDI6044681.1"
                     /translation="MGRPLKTLFKREHSEEISNPSDNRAFSQVAEVYLSRRRLLQAGT
                     VAGAAAAFPFLLKQENALAKVVSKASGNLGKATTLGFTSLPVSTEDTVIVPEGYIAKP
                     FYKWGDATGIAGNLPVFKTDGSNTTEEQAAQAGMHHDGMAWFSLPQGGNSSDHGLLAI
                     NHEYIDNGLLFKDGDANWSADKALKGQNAMGVSVIEVKKVPLGWEVVRPSSFARRITV
                     NTPMKITGPALHNPLMQTVDDPKGEIILGTMQNCANGFTPWGTYLTCEENWSDIFVKK
                     AEMNPLEKRYGISGSDDSYRWNEVDKRFSVDATPNEPNRFGWVVEIDPYDPHSVPRKH
                     TALGRIKHEGAAVTIAPDNRVVVYMGDDQKFEYIYKFVSEGKFNPQDRKANMHLLEKG
                     TLYVAKFNDDGKGEWLPLVFGQNGLDASKGFENQGDLLIKTRLAADAVGATKMDRPEW
                     IAVDPYHAGSVYCTLTNNSDRGKEGKAPVDAANPRAKNVYGHIIHWLEHNGDPTALQF
                     AWDILVMGGRTDTDKLETKGSMKGAEFGSADGLSFDHRGVLWIQTDVSSSTINIKDYK
                     GMGNNQMVATIPGTNEFRRFLTGPRGCEITGIAFTPDNRTLFINIQHPGEPSEGLSDP
                     QHPTAVSSWPDGDKAGRPRSSTVVIVKADGGIIGT"
     gene            7520..>7606
                     /locus_tag="PN977_004752"
     CDS             7520..>7606
                     /locus_tag="PN977_004752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HDI6044682.1"
                     /translation="MKKRNFSAEFKRESAQLVVDQNYTVADAA"
BASE COUNT         1896 a         1943 c         1855 g         1912 t
ORIGIN      
        1 gtaatgatat gaaaatactg gcggccagaa aagcagtata ccagctggca cgcgaacagc
       61 atcctgaacg ttggggaaag catctacgta actgggacta catatcagaa gtctacctga
      121 atccagaaaa agaggctgca taacgtaatt ttattacaaa aggcgacaac taccttgaaa
      181 aacgccgtta ttgagtgatc tcctttcatg aaaatacgca ccatgaccat accctctgca
      241 acctgaccat cgtagtcacg cagcgtcagt gaacctccga acaactgttt tacccggtac
      301 atcgctgttt ccgctatcga acgacggtta taatctgttg tccatttaca ccgggcatta
      361 cttccggtca gccgctgatt agccactgca cggttacggt cttcgtattc accgggctag
      421 taacccgcac cttttcggga aggggtaagc gcgctgattt tcttacgcct cagttcatcg
      481 tgacagagtc gggggtcgta agccccgtct accgctgcag ccatgatttt tctgtgagtc
      541 tgttggagaa gccccgggaa ggcttctgag tccgtgacgt tgttcagcga caggtctgca
      601 cagacaattt catgtgtgtt gccatcaaca gacagatgcg acgcccctct ttgccgtgct
      661 ttttggcttt cctttcgcct tccccaaaga cttttagccc gttggaatca atcacgaggt
      721 gtatgatttc accccgggtg gggatttatg tataagaaac agcgtattcg tgaggggtga
      781 gccatgaata aaatcccttt tgaagtatta atccactctg aaaatgcatt aatctgcgca
      841 aaggaaatgg acgcgttatt aattaagtta attgatattc cggaaagtgg cgataaaaca
      901 tattcgatga cggacaggcg acaatgtggt tatctgtgct ggcgtttggc agacaggccg
      961 aagcactggc cagacaccag aaaggcgaac tggtaagcgt ggcaggtaac atgcaggtca
     1021 gccagtggac agggcagaac gggaaaacgc ggcagggacg gcaggttatc gcagacagcg
     1081 taatcaatac tcaaacggtg cggccaggag gaagcagacg cgaaaccaca ggtacacagg
     1141 gtaatcagcc accaacggga ggcgacgcca cttacggaga tgatataccc ttatgaatta
     1201 agcggggcgc aacatgaaca acagcacact gaagagccgg acaataagcg acgcgacagc
     1261 ggaagaactc gagagcaggg gactgtggcg acgtgctgcc agaaggtggc aggatgtgat
     1321 gattactctt gagaaagaca gtcacagaca gcttgcagta atgagagcaa gggaatgtat
     1381 caggaaggca aaacgcccga tacctgaaag ccgtattgat attcatacag tcagaaaggc
     1441 cgcagacagg acaatacaaa aaatggggct accgtcgctt actgatgaaa tatggcgcga
     1501 ttaccctgat agcgttaacg atgacggtta ttaactggcg ggggaaacac gataccagcg
     1561 ggcgtgactc gggagaatat ataccgctgg tggaacatta ccggccctga tggctggtgg
     1621 cgtgcgttac gacctgaaag accatccatg cgggaaggtg acacaggagc acctggcgaa
     1681 cgcggggaag cgactggatg agattaagaa aaaacagcag gagcgcagaa aaagtaaccg
     1741 tctcattaaa acgtctttcg cctccctttc ctgtttccta tgctaatgtc cattttcgca
     1801 gtaaaaggac atttacgatg aatgcacgaa aggcggtact ggcagataat ccggaattga
     1861 tcctgcgttt gctacaactg aaatttgacg agacactgtc gtacccgcgc atttctgcac
     1921 agaccggtgt cagcaaaacc gctatttttt ctctggtgag gcgatttcat caggtattca
     1981 ctgacgggca tctttctggt gaatattcct gcgtgcaact gaccgggctc ttttcccggg
     2041 gcgataatat tcagccccga cagtgactca gcctgtaaaa acagagaaaa cccgtcggaa
     2101 ccgattttca ccagagtaaa aatggagact tgttcagcaa actcttttac ccggagcctg
     2161 tgtggcccag attgcacgag agtctggcat caatgataat gttattttca aatggctcag
     2221 actctggcaa aacgaagggc gtgtttcgcg gcgtcttccg gtaacaacca cttctgacgc
     2281 aggcgttgaa ttattaccta tggagataac gccggatgag cagaaagatc cgatgattcc
     2341 cctaccgcca gcttttcgcc tgcgccggta tacgtttctt tatctttgcc ctgcatcacc
     2401 cacaggctgg ctccgccgat aatggcgttt tgtggtgcat ctttcgcgtc agcgtcgtaa
     2461 ggcatcatgc ctacgccgta gttgaatttg gcgtactcgc gaatgttggc aagagaaccg
     2521 gaagaggcgg tggtcatcgc gcaatcacct ctatagaact tctcggtgga ttcatcctta
     2581 cgcccgacgt agatgaagtc gcccttcttg ttcatctcct cgagcatgac gatgtgtttc
     2641 acctgctccg gcttgttgaa ctccagcacc gcgtccgtgg cgtcaaaacc gttgtttttg
     2701 ctggcaaacg gcagaccgtt cctggcgcta aagttttcca gttgtatcca cctctttgcc
     2761 catttcccct tccatagagt tccaaaacgg aatggtcgtc actgcctgtg cattgcccat
     2821 taacgccagt ccgagcacca gtgatgaagc tgtataatgt aacggtttca tcgtttatct
     2881 ctcttgcttg ttgtacctga tgtgcaaatt cactcgtttt atgctcgcgg gattacatat
     2941 catgcccgaa ttgcagaaaa ataacttttt attacatgtg taagacagta aaacctcaga
     3001 aagatgacag tgtggtgccg gcgcgggtga ggaaaaatgg aagatgggga atgtataagc
     3061 gggaaaatag gagggcctgt ggattgtaga taatgtgctc atcgttttca tgccggatga
     3121 gacataaaca cttttatctg agcatttcta tgtattcgga cagttgttct tggcatccaa
     3181 tggtgcaggt tctgtagtgt gtggtggtag caatagcctc agcttcatta caatatttat
     3241 tatctcaaac attcaaatgt ccttggccca ctttgatagt cactttgtcg tggttattat
     3301 gtctttcctt ttcctaaagc cccattatct gtgctgcagc tttccctcca tcaacgaacc
     3361 aacaaccagc aatgcctgtc ccccataaca gcccggaaaa tggcacattc tcagatgatt
     3421 tatcccgcga gctatcccgc ctacagctaa tcgacctgtt taatacatct tgacagtctg
     3481 ctggcaaggt tacccttccc aatattgtcc gtttttaaca tatctaatac gagcccccat
     3541 caaatatgct tcgtgttcac tgtaagtgac taaaagtgct gtctgccctg cctcatgcaa
     3601 aatatcacga acttcacggc tcagtcctac ccttgttttt tatccaagct ggaaaatggt
     3661 tcatctagca acaggatatc tggatttgga accagtgacc ttgcaagggc aatacgttgc
     3721 tgctgacctc ctgaaagttc atgaggatag ctgtctgaat agtggaataa atccaccatt
     3781 ttcaacagtt ctgtaactct ggttcgctgt acatctctcg gcagtcttct cagaccaaag
     3841 ccacgttttg tgaaactgta aggtggggaa agagcgcata ttcctgaaag actataccga
     3901 catgacattt ctccggcgga aaatgcatat ctgcgctggc cacacaatgc tgtgatacaa
     3961 aaatttcccc ttgttcaggc gctcaaaacc agctacagca cgtagtactg ttgtttttcc
     4021 acaacctgat gtaccaagca gacatgccag ttcaccttta cagactgaca taccccccgc
     4081 cccgcaatac aggatatggg ctattgggat aaacaacatg gacatcatta atacataaag
     4141 ctgtttcttc agacataatc aatcctctga tttcagtctt tcagctggaa caaacaaaat
     4201 aaccggaaat gtccctgcaa gaacaatcat taaagcagag atggcccctt cttcaaaagt
     4261 tccacgggct gcttcagcat aaagaagtgt tactggcagt tctttcatcg catcagtgaa
     4321 aaccagcagg gcactgctca ccagagcaac acaggaaagt gaacccgaac aaaagttcct
     4381 gtaccatttt ctcccagcag acgtgaggct tgttccagtg actctggtat acgtcccagc
     4441 cggacatcaa gtgcgccgat ggctattgcc tgaaatcgca tcgtacagca aatcaccaac
     4501 aggacaccga cagacattaa aggtaacccc ctgtaatcca gtagttccgc cagccagcgg
     4561 tcatttaggg agtcagaaaa ccaatcgcca gaatcgtgcc tggaaaggca taacccagcg
     4621 acgccactct catcacaccg cgcctgaaca gtggcatagc gttaacccag cgacgccact
     4681 ctcatcacac cgcgcctgaa cagtggcata gcgttgagga attccgggca ttccagacta
     4741 cgagcagact gacaaaaatt acgacaaagc gttgaaggaa gccagcgcgc ggactgacag
     4801 gactttacca taaaaacaca ggctaaccgc gcctgcgttt ttaatgacgg atgaaacaga
     4861 atcaggtgcc gattatgccg ccgtcggctt ttacgataac gacagttgag gagcgcggac
     4921 gaccagcctt atcgccgtca ggccagcttg atactgccgt cggatgctgc ggatcgctaa
     4981 gcccttcgct tggctcgcca gggtgctgga tgttaataaa cagcgtccgg ttatccggag
     5041 tgaacgcgat gccggtgatt tcgcagccgc gcgggccggt caggaagcgg cggaactcat
     5101 tggtaccggg gatcgtcgcc accatctggt tgttgcccat gcctttgtaa tctttaatat
     5161 ttatggttga tgacgacacg tcggtctgga tccatagcac gccgcggtga tcaaagctca
     5221 ggccatcggc gctgccgaat tccgcaccct tcatgctgcc tttggtttcc agtttgtctg
     5281 tatcggtgcg accgcccatc acaagaatat cccaggcaaa ctgcagcgcg gtcgggtcac
     5341 cgttatgctc caaccagtga atgatgtggc cgtaaacgtt tttggcgcgc gggttggccg
     5401 catccactgg cgcttttcct tccttaccgc gatcgctgtt gttggtgagc gtgcagtaaa
     5461 cgctgccggc gtgatatgga tcgaccgcaa tccactccgg gcggtccatt ttagtggccc
     5521 ccacggcgtc tgccgccaga cgggttttaa tcaacaggtc gccctggttt tcaaaccctt
     5581 tactggcgtc gagcccattt tgtccgaaca ccagcggtag ccactcgcct ttgccgtcgt
     5641 cgttaaattt cgccacgtac agagtgcctt tttccagcag gtgcatgttg gctttgcgat
     5701 cctgcgggtt gaattttcct tccgagacaa atttatatat gtattcgaat ttctggtcat
     5761 cgcccatata gaccacaacg cggttatccg gggcaattgt caccgctgcg ccttcgtgtt
     5821 tgatgcgacc aagcgcggtg tgcttgcgcg gcactgaatg cggatcgtat ggatcgattt
     5881 ccaccaccca gccgaagcgg ttcggctcgt tcggcgtggc gtcgacgctg aaccttttat
     5941 ccacttcgtt ccagcggtaa gagtcgtcgc tcccgctgat accgtaacgt ttttccagcg
     6001 ggttcatctc tgcttttttt acgaaaatat cggaccagtt ctcttcacac gtcagatagg
     6061 tgccccacgg cgtaaatccg ttggcacagt tctgcatggt gccgagaatg atttcgcctt
     6121 tcggatcgtc aactgtttgc atcagtgggt tatgcagcgc cggaccggtg attttcatcg
     6181 gcgtgttaac ggtgatgcgg cgtgcgaacg atgacgggcg caccacttcc cagcccaggg
     6241 gcactttttt tacttcaatg accgacacgc ccatcgcgtt ctggccttta agagctttat
     6301 cggcgctcca gttggcatcg ccatctttaa acagtagacc gttatcgatg tattcatggt
     6361 ttatggcaag caggccgtga tcggagctgt tcccgccctg cggcaggcta aaccacgcca
     6421 tgccgtcgtg gtgcatcccg gcctgcgccg cctgttcttc cgtggtgttg ctaccgtcgg
     6481 ttttaaaaac cggcagattt ccggcaatcc cggtggcgtc gccccatttg tagaagggct
     6541 tcgcgatgta tccttccggc acgataacgg tatcttccgt ggaaaccggc aggctggtga
     6601 aaccgagcgt cgtcgctttg ccgaggttac cgcttgcttt ggagaccact ttcgccagcg
     6661 cgttttcctg tttcagcaga aatgggaacg ctgccgcggc accggcgacc gtgcctgcct
     6721 gtaacagacg gcggcgtgac aaatagactt ccgccacctg cgaaaacgcg cggttatcgc
     6781 tcgggttact gatttcttca ctatgctcac gtttgaataa agtttttaac ggtctgccca
     6841 ttgcggtttc ctttcaaaaa atcgatttca aggggaatca tttttatgca cgtgagtcta
     6901 cggaaagatt gttacggaaa tataacaatt gtcataaaaa accggcgata aaatcatcta
     6961 tggtcgggac gcggtttcag gtatgatgtg aggctagaat tctgaacgct ctcttttgtt
     7021 gagcatatat ggacgtccca gatgaggcaa gtactggttc gatgcggttt gcgaccatat
     7081 atccggcgtc gttagtaggt tcaatgacct gcgccatgat gttatccggg ccctatttcc
     7141 taatcactac accggtattt gattacccag gtcaacttca gactgttaca ggggcggtag
     7201 ccgttatctc atcagtgaat gtaaactcag ttaaaaatat tttctataac ttactgtttt
     7261 tctttaattc atcacaacgg ccttctgcat attaaaatca atactatcgc tcaaaacacg
     7321 ccagatgatt cttgtcagct tattcgccag cgcaccagtg gttttcagga accctcgacg
     7381 ggcttccatc ctttgcagac aaatactcag actgtcagtg agctttttca cacagcgcat
     7441 tacagactga aatggcacac tgaatttggt cacctgaaca gaggtgatat gctcacctca
     7501 gaacaagact ggtgccctaa tgaaaaaaag aaattttagc gcagagttta aacgcgaatc
     7561 cgctcaactg gttgttgacc agaactacac agttgcagat gccgcc
//
LOCUS       DAMZFT010000201         7126 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.201, whole genome shotgun sequence.
ACCESSION   DAMZFT010000201 DAMZFT010000000
VERSION     DAMZFT010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7126)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7126)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7126
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.201"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            515..1954
                     /locus_tag="PN977_004753"
                     /pseudo
     CDS             515..1954
                     /locus_tag="PN977_004753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000961341.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5507 domain-containing protein"
     gene            2285..2551
                     /locus_tag="PN977_004754"
     CDS             2285..2551
                     /locus_tag="PN977_004754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000340076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDI6044683.1"
                     /translation="MLRVTTAAHNRGSRPGQNKRLRTLSVGDALLVCKLNRLGRSMWH
                     LVVLLEELCERGINFRALAQSIFAQQWGDGSCKSKTICDLRVIV"
     gene            2706..2825
                     /locus_tag="PN977_004755"
     CDS             2706..2825
                     /locus_tag="PN977_004755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001131457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HDI6044684.1"
                     /translation="MPHSYAEYHHFIMMSLMSGEHNTLALSLQDESPGLVPVG"
     gene            2890..>7126
                     /locus_tag="PN977_004756"
     CDS             2890..>7126
                     /locus_tag="PN977_004756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311542.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA repeat-containing protein"
                     /protein_id="HDI6044685.1"
                     /translation="MNRTSLHYCRRSVLSLWISALIYAPPGMAAFTPDVIGVVNDETV
                     DGSQKVDERGTTNNTHIINHGQQNVYGGISNESLIESGGYQDIGRHNNYVGQANNTTI
                     NGGRQTIHDGGISTGTIIESGNQDVYTGGISNGTTIKGGNSHISGGTANGTIIDGGSQ
                     RVTTQGHVDSTTINKSGSQDVVQGSLATNTTINGGRQYVEQSTVETTTIKNGGEQRVY
                     ESRALDTTIEGGTQSLNSNSTAKNTQIYSGGTQIVDYTSTSDVIEIYSGGVLDVRGGM
                     ATNVTQHDGAALKVTTYDLTVSGTNSEGAFSIHNNVADNVLLENGGHLDVYGSATRTI
                     IKDKGTMSVLTNAKADATRIDNGGVMDVAGNATNTIINGGTQNINNHGIATGTNINSG
                     TQNIKSGGKADTTNISSGSRQIVEKDGTATGSNISAGGSLIVYTGGIAHGVNQETGSA
                     LVANTGAGTDIEGYNKLSRFTIIGGEANYVVLENTGELTVVAKTSAKNTTIDAGGKLI
                     VQKEAKTDSTRLNNGGVLEVQDGGEAKHVEQQSGGALIASTTSGTLIEGTNSYGDAFY
                     IRNSEAKNVVLENAGSLTVVTGSRAVDTIINANGKMDVYGKDVGTVLNSAGTQTIYAS
                     ATSEKANIKGGKQTVYGLATEANIESGEQIVDGGSTDKTHIKGGTQTVQNYGKAINTD
                     IVSGLQQIMANGTAEGSIINGGSQVVNEGGLAENSVLNDGGTLDVREKGSATGIQQSS
                     QGALVATTRATRVTGTRADGVAFSIEQGAANNILLTNGGVLTVESDTSSAKTQVNAGG
                     REIVKTKATATGTTLTGGEQIVEGVANETTINDGGIQTVSANGEAVKTTINEGGTLTV
                     NDNGKATDIIQNSGAALQTSTANGIEISGTHQYGTFSIAGNLATNALLENGGNLLVLA
                     GTEARDSTVGKGGAMQNLGQDSATKVNSGGQYTLGRSKDEFQALARAEDLQVAGGTAI
                     VYAGTLADASVSGATGSLSLMTPRDNVTPVKLEGAIRITDSATLTIGNGVDTTLTDLT
                     AASRGSVWLNSNNSCAGTSNCEYRVNSLLLNDGDVYLSAQTAAPATTNGIYNTLTTSE
                     LSGSGNFYLHTNVAGSRGDQLVVNNNATGNFKIFVQDTGVSPQSDDAMTLVKTGGGDA
                     SFTLGNTGGFVDLGTYEYVLKSDGNSNWNLTNDVKPNPDPNPNPKPDPKPDPKPDPKP
                     DPTPDPTPTPVPEKRITPSTAAVLNMAATLPLVFDAELNSIRERLNIMKASPHNNNVW
                     GTTYNTRNNVTTDAGAGFEQTLTGMTVGIDSRNDIPEGIATLGAFMGYSHSHIGFDRG
                     GHGSVGSYSLGGYASWEHESGFYLDGIVKLNRFESNVAGKMSSGGAANGSYRSNGLGG
                     HIETGMRFTDGNWNLTPYASLTGFTADNPEYHLS"
BASE COUNT         2153 a         1440 c         1745 g         1788 t
ORIGIN      
        1 aacgataaag ccagagtatt atcatttatc atgccttttc atttaatcag gttaattact
       61 caaatcactg aactattctc tgttcgctca ctgatgataa gtgaagtgtc acaaccacga
      121 tttgtatgtg gaccaatgcc gtagagtaag gatgaaatgt ctctgccacg ccttaaacat
      181 ctactactga ccagaccacc cgtcgcaggc agatgagtct tatgagcgtt acaggagtct
      241 ggctttgctc attaattaac gctatttaga tataaatttc taacactcga aaaacgaatt
      301 ccactaataa atcataacgg ttggttttta tcatacagcg aaataacttt ttgtttatta
      361 aatttaacat tcttgtgcta atttaattaa aaagtccatg cgtagcaagt gaaacagtta
      421 atgtcagcaa ttgtctcaaa tgagcggttt tttatagtga caaacagtaa taacgttaaa
      481 catagccatg tagacattaa ctgaggaata gacaatgtta gttagtaaaa gtaatggtca
      541 taacgttaat acagttttgg gtagtggaag ttgtaatgaa aacaattctt ccaaccccat
      601 ggagctacta gcttatagta ttataaattt aatttgtaag gaagctgcct cagggatgta
      661 tcgcggtgtt cttgaaactt tacaaaaaat aatgtctgaa tgtatatatc aagaaggcaa
      721 cgcctttgtc attatgggag ctggagaaca attaaaacgt attaaatatg atgttgatga
      781 aaataactta aaagtattca acgtacactt tgataataat gaagagttag ttactgatgg
      841 tgagcctgac gtagtatgtt taagcaagta ggagtgggaa gatcttctca ttaaactaaa
      901 gctggagaac aaagaaaatg cggtttctga aactgacctg tcatcgaata aaaataatgt
      961 tgatcagttt tttgaatacc ataaaagaaa tgaatagaac attttcgata atataagaaa
     1021 aagtgatttt catgttggtt cacttcagcc aggtagaagg cgtagtgtta ttttagaaac
     1081 gccaacaaat gtctgtgtgg aaccacataa tttatatgac aaccaaatat ataaagtttc
     1141 acctgtaact aaaaactctc agcaagttaa aggccactat ggagataaat tgaaagaaat
     1201 gcagatgttc cttaaccaga taagcaatgc acttcaacag gctccatctt tgtcagagtc
     1261 aaaggaacac accatagata ttcaaaaaat aacggatgcc tttgtgaagg agttccgtgg
     1321 gatattattt gataaaaatg gaaattcttc agagcgttta ttcaattttt atgagtgctg
     1381 ctatatattt ttaccaagag cgcagcctca agataaaatc gaaagctata attcagcact
     1441 gcaagctttt tccatctttc gttcatctac tttgaataat aatgatgtag ggtttaattt
     1501 caaattattc ccagaagtca aactgcctgg ggaaaatctt gaaacggtat tcaaatacaa
     1561 aaaaggaagt tttgtccggg agatagccag aattaacatt gctctacaaa aaggagaggg
     1621 tggtttatat aatttaggtg ggttggattt taagggatgc ttcttttctg gacagaactt
     1681 cagtaactat gatattcaat atgtgaactg ggggacgtca ttgtttgatc ttgatactcc
     1741 atgtattttt aatgcgcctg cttacaacaa gagtaatgaa aaatcattag aacgcgtcag
     1801 cgaaaacggt ttaagtggag tcttgtctga tcgtaataaa aaaataaaac tcatcacggg
     1861 cgtggcacca ttcgatgata ttttatttat ggatgatgac tttgatgatc cttccactaa
     1921 ggatgatccc gtggagaata gtccccttgt ataaagttct aaaagcagtt ttcaatgata
     1981 aataaatggc gatttttaca aagagataat aacgtagatt gaaataatat gtattattga
     2041 ttgagtaaac gggattattc ttcacactgc atccattttg ctgtcactac caaagtaaaa
     2101 ggggctgcga tttcacgtaa tcccttgatt tatttgataa gttggcggtg tctgaactgg
     2161 tgctgtaatt ggttgaaata tttcatctaa tgtctggttc aactttcctt agctgcttga
     2221 aagttttttg atcttaccca gcaataccag cgttacggtg caccacgcct gactgaataa
     2281 ctgcgtgctt agagttacca ctgcagcgca taaccgtggc agcaggcccg gacagaacaa
     2341 acggctcagg acattatcgg taggggacgc actgctagtc tgtaaactga accgactggg
     2401 gcgcagtatg tggcatcttg tcgtgctgtt ggaggagctg tgcgaacgtg gtattaactt
     2461 tcgtgctctg gcccaatcta tatttgccca acaatggggg gacggaagct gtaaaagtaa
     2521 aacaatctgc gatctcagag ttattgtgtg atttttatgt gagcagaaga tattcatcag
     2581 aaacgattat gtaaatcatt ttattttgcc gactgcctga ttgtcgaaag aaaagaatac
     2641 gcccgtgttt cctgaataga ttgacttttt tatccaacca cacttcagcg cactgcgttt
     2701 aaaaaatgcc tcattcttat gcggaatatc atcatttcat catgatgtct ttgatgagcg
     2761 gtgaacacaa tacacttgcg ctgtctcttc aggatgaatc ccctggtctg gtgcctgtgg
     2821 gctgatgttg cagcaaagct aaggtcgctt aaacgctact cagtattcac ttttcagagg
     2881 atgaaattta tgaacaggac cagtctccat tattgtcgcc gctcagtact ttccttatgg
     2941 atatctgcct tgatatatgc cccgcccggg atggcggcct tcactcctga tgttattggt
     3001 gtggtaaacg atgagactgt agatggcagc caaaaagtag atgaacgagg tacaacaaat
     3061 aacactcata ttatcaacca tggccagcag aatgtttatg gtgggatatc taatgaaagt
     3121 cttattgaat ctggtggata tcaagatata ggacgtcata acaattatgt ggggcaggct
     3181 aataatacaa ccattaacgg gggcagacag acaattcatg acgggggtat ttccacaggt
     3241 acaataatcg agagtggcaa tcaggacgtt tatacaggtg gtatcagcaa tggaacgaca
     3301 attaaggggg gtaattcaca cataagtggg gggactgcga atggaacaat cattgatggt
     3361 ggcagccaac gagtaacaac tcaggggcat gtcgacagta caacgataaa taagtctggc
     3421 tctcaggacg tagtacaagg aagtctggca acgaacacaa ccataaatgg tggtcgacag
     3481 tatgttgaac agagcacagt agaaacaacc accatcaaaa atggcggtga gcaaagagta
     3541 tatgagagcc gtgcgctgga cactacgatt gaaggcggaa ctcagtctct gaatagtaat
     3601 tcaacggcaa aaaatacgca gatctattct ggtggtacgc aaattgttga ttacaccagc
     3661 acctcggatg ttattgaaat ttattccggt ggcgtgcttg atgttagagg tggtatggca
     3721 acaaatgtta ctcagcacga tggagccgct ttaaaagtaa cgacttacga tttgacggtg
     3781 agcggtacga atagtgaagg ggcattctcc atccacaata acgtggcaga caatgtgttg
     3841 ctggaaaacg gtggtcattt agacgtatat ggttcggcaa ccaggacgat aattaaagat
     3901 aaaggaacaa tgtcagtttt aacgaatgct aaagctgatg cgacccgaat agataatggc
     3961 ggggttatgg atgtcgccgg aaacgcgacc aataccataa tcaatggtgg tacacagaat
     4021 attaataatc atggtattgc cactggcacc aatatcaaca gtggaacgca aaatatcaag
     4081 agcggcggga aagctgacac gacaaatata tcctccggga gccggcagat tgttgagaaa
     4141 gatggtacgg caactggcag caatattagc gctggaggct cgctgattgt ctataccggc
     4201 ggtattgcac atggggttaa ccaggagacg ggcagtgctt tagttgccaa cacgggcgca
     4261 gggactgata tcgaaggata caacaagctc tctcgcttca ctattatcgg aggagaggct
     4321 aattatgttg tgctggaaaa taccggcgaa ctgacggtag tggctaaaac ctcggcgaaa
     4381 aatactacca ttgatgctgg cggtaagctg attgtccaga aggaggctaa aacagatagc
     4441 accagactta ataatggcgg cgttctggag gttcaggacg gtggtgaggc taagcatgtt
     4501 gagcaacaat ccggcggcgc attaattgct tccacgacct ccggaacact tatcgaagga
     4561 accaacagtt atggtgatgc tttctacatc aggaattcag aagctaaaaa tgtagtgctg
     4621 gaaaacgctg gctcattaac agtcgtcact ggttcccggg cagttgacac gattattaat
     4681 gccaacggca aaatggatgt ttatggaaaa gatgttggta ctgtacttaa tagtgcgggt
     4741 acccaaacaa tatatgccag tgccacttct gaaaaagcaa atatcaaagg tggcaagcaa
     4801 acggtatatg gtttagccac tgaggcaaat atcgaaagtg gtgaacaaat tgttgatggt
     4861 gggtcaacag ataaaacgca catcaaaggc ggcacgcaaa ccgttcagaa ttatggtaag
     4921 gcgatcaata ccgatatcgt ctctggccta caacaaatta tggcaaacgg gacagcggaa
     4981 ggttccatta ttaatggcgg ttcacaggta gttaatgagg gcggtctggc tgaaaactcg
     5041 gtacttaatg acggcggcac actcgatgtg cgggagaaag gcagcgctac ggggatacag
     5101 cagagtagcc agggcgcgtt ggtggcaacc accagagcga cgcgggtcac agggacacgc
     5161 gctgatggcg tcgcgttcag catcgagcag ggtgcggcga acaatatcct gctgacaaat
     5221 ggcggcgtgt taaccgtgga gtcagacacc tcttccgcca aaacacaggt caatgcgggc
     5281 gggcgggaga tcgtcaaaac aaaagccact gcgacaggca cgacgctcac cggcggcgaa
     5341 caaatcgtcg agggtgtggc gaatgagaca acgattaacg acggcggaat acaaacagtt
     5401 tcggctaacg gtgaggcagt aaaaacaacg attaatgaag gcggtacgct gacagtcaac
     5461 gataatggca aagcgacaga tatcatccag aacagcggtg ccgctctcca gacgagcacg
     5521 gctaacggta ttgaaatcag cggtactcac cagtacggca ccttttccat tgccggaaat
     5581 ttagcgacca atgcgttgct ggaaaatggc ggtaatttat tggtattagc aggtaccgaa
     5641 gcccgtgact ccacggttgg caagggtggt gcaatgcaaa acctgggtca ggactccgcc
     5701 acaaaggtta actctggcgg gcaatatacc cttgggcggt caaaagatga gtttcaggct
     5761 ctggcccggg cagaagatct ccaggtcgct ggcgggacgg caatcgtcta tgcaggtacg
     5821 ctggcggatg catcggtcag tggcgcgaca ggaagcctgt cgttaatgac gccacgggat
     5881 aatgttacgc cagttaaact cgaaggggcg atccggatta ccgatagcgc gacattaact
     5941 atcggaaatg gcgtcgatac gacgcttacc gacctgacgg ctgccagccg gggcagtgtc
     6001 tggcttaaca gcaataattc ctgtgcaggt accagcaact gcgaatatag agtaaacagt
     6061 ttgctcctca acgacggtga tgtttattta tcagcacaaa cagcagcgcc tgccacaact
     6121 aacggtatct acaatacgct gacaaccagt gaactttccg gcagcggtaa tttctacctg
     6181 cataccaacg ttgcaggctc ccggggcgat caactggtcg tcaacaacaa cgccactggt
     6241 aattttaaaa tctttgttca ggataccggc gtcagccctc agtctgacga cgcgatgacg
     6301 ctggtgaaaa cagggggagg ggatgcttcg tttacgctgg gtaataccgg cggtttcgtt
     6361 gatcttggga cctatgagta tgtcctgaaa agcgacggca acagcaactg gaacctgacc
     6421 aatgatgtca aacccaaccc ggaccccaac ccaaatccga agccggatcc aaaaccagac
     6481 ccaaaaccgg atccgaaacc agacccgact cccgatccaa cgccgacacc cgtcccggag
     6541 aaacgcatca cgccttctac ggcagccgta ctcaatatgg cagcaacatt accgttggtc
     6601 tttgatgctg agctaaacag tattcgcgag cggttgaaca taatgaaagc gagtccacac
     6661 aacaataatg tctgggggac gacgtataac acccgtaata atgtcaccac cgatgcgggt
     6721 gccgggtttg agcagacgct gaccggaatg acggtgggga tcgatagccg taatgatatt
     6781 cctgagggga ttgcgacgct gggcgctttt atgggttatt cccattcaca tatcggtttt
     6841 gatcgtggag gacatggcag tgtgggcagt tattctctgg ggggctatgc cagctgggaa
     6901 catgaaagtg gtttctatct ggacggtatc gtgaagctga accgctttga aagtaacgta
     6961 gccggtaaaa tgagcagcgg tggagccgcc aacggcagtt atcgcagcaa cgggctgggc
     7021 ggtcacattg aaaccgggat gcgatttacc gatggtaact ggaacctgac gccgtatgca
     7081 tcgttaacgg ggttcaccgc tgataacccc gaatatcatt tatcca
//
LOCUS       DAMZFT010000202         7037 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.202, whole genome shotgun sequence.
ACCESSION   DAMZFT010000202 DAMZFT010000000
VERSION     DAMZFT010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7037)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7037)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7037
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.202"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            464..4207
                     /locus_tag="PN977_004757"
     CDS             464..4207
                     /locus_tag="PN977_004757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit alpha"
                     /protein_id="HDI6044686.1"
                     /translation="MSKFLDRFRYFKQKGETFADGHGQLLNTNRDWEDGYRQRWQHDK
                     IVRSTHGVNCTGSCSWKIYVKNGLVTWETQQTDYPRTRPDLPNHEPRGCPRGASYSWY
                     LYSANRLKYPMMRKRLMKMWREAKALHSDPVEAWASIIEDADKAKSFKQARGRGGFVR
                     SSWQEVNELIAASNVYTIKNYGPDRVAGFSPIPAMSMVSYASGARYLSLIGGTCLSFY
                     DWYCDLPPASPQTWGEQTDVPESADWYNSSYIIAWGSNVPQTRTPDAHFFTEVRYKGT
                     KTVAVTPDYAEIAKLCDLWLAPKQGTDAAMALAMGHVMLREFHLDNPSQYFTDYVRRY
                     TDMPMLVMLEERDGYYAAGRMLRAADLVDALGQENNPEWKTVAFNTNGEMVAPNGSIG
                     FRWGEKGKWNLEQRDGKTGEETELQLSLLGSQDEIAEVGFPYFGGDGTEHFNKVELEN
                     VLLHKLPVKRLQLADGSTALVTTVYDLTLANYGLERGLNDVNCAASYDDVKAYTPAWA
                     EQITGVSRSQIIRIAREFADNADKTHGRSMIIVGAGLNHWYHLDMNYRGLINMLIFCG
                     CVGQSGGGWAHYVGQEKLRPQTGWQPLAFALDWQRPARHMNSTSYFYNHSSQWRYETV
                     TAEELLSPMADKSRYTGHLIDFNVRAERMGWLPSAPQLGTNPLTIAREAEKAGMNPVD
                     YTVKSLKEGSIRFAAEQPENGKNHPRNLFIWRSNLLGSSGKGHEFMLKYLLGTEHGIQ
                     GKDLGQQGGVKPEEVDWQDNGLEGKLDLVVTLDFRLSSTCLYSDIILPTATWYEKDDM
                     NTSDMHPFIHPLSAAVDPAWEAKSDWEIYKAIAKKFSEVCVGHLGKETDIVTLPIQHD
                     SAAELAQPLDVKDWKKGECDLIPGKTAPHIMVVERDYPATYERFTSIGPLMEKIGNGG
                     KGIAWNTQSEMDLLRKLNYTKAEGPAKGQPMLNTAIDAAEMILTLAPETNGQVAVKAW
                     AALSEFTGRDHTHLALNKEDEKIRFRDIQAQPRKIISSPTWSGLEDEHVSYNAGYTNV
                     HELIPWRTLSGRQQLYQDHQWMRDFGESLLVYRPPIDTRSVKEVMGQKSNGNPEKALN
                     FLTPHQKWGIHSTYSDNLLMLTLGRGGPVVWLSEADAKELGIADNDWIEVFNSNGALT
                     ARAVVSQRVPAGMTMMYHAQERIVNLPGSEITQQRGGIHNSVTRITPKPTHMIGGYAH
                     LAYGFNYYGTVGSNRDEFVVVRKMKNIDWLDGEGNDQVQESVK"
     gene            4204..5742
                     /gene="narH"
                     /locus_tag="PN977_004758"
     CDS             4204..5742
                     /gene="narH"
                     /locus_tag="PN977_004758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HDI6044687.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTSREGVEYAWFNNV
                     ETKPGQGFPTDWENQEKYKGGWIRKINGKLQPRMGNRAMLLGKIFANPHLPGIDDYYE
                     PFDFDYQNLHTAPEGSKSQPIARPRSLITGERMAKIEKGPNWEDDLGGEFDKLAKDKN
                     FDNIQKAMYSQFENTFMMYLPRLCEHCLNPACVATCPSGAIYKREEDGIVLIDQDKCR
                     GWRMCITGCPYKKIYFNWKSGKSEKCIFCYPRIEAGQPTVCSETCVGRIRYLGVLLYD
                     ADAIERAASTENEKDLYQRQLDVFLDPNDPKVIEQAIKDGIPLSVIEAAQQSPVYKMA
                     MEWKLALPLHPEYRTLPMVWYVPPLSPIQSAADAGELGSNGILPDVESLRIPVQYLAN
                     LLTAGDTKPVLRALKRMLAMRHYKRAETVDGKVDTRALEEVGLTEAQAQEMYRYLAIA
                     NYEDRFVVPSSHRELAREAFPEKNGCGFTFGDGCHGSDTKFNLFNSRRIDAIDVTSKT
                     EPHP"
     gene            5739..6449
                     /gene="narJ"
                     /locus_tag="PN977_004759"
     CDS             5739..6449
                     /gene="narJ"
                     /locus_tag="PN977_004759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HDI6044688.1"
                     /translation="MIELVIVSRLLEYPDAALWQHQQEMFEAIAASKNLSKEDAHALG
                     IFLRDLTAMDPLDAQAQYSELFDRGRATSLLLFEHVHGESRDRGQAMVDLLAQYEQHG
                     LQLNSRELPDHLPLYLEYLAQLPQSEAVEGLKDIAPILALLSARLQQRESRYAVLFDL
                     LLKLANTAIDSDKVAEKIADEARDDTPQALDAVWEEEQVKFFADKGCGDSAITAHQRR
                     FAGAVAPQYLNITTGGQH"
     gene            6449..>7037
                     /gene="narI"
                     /locus_tag="PN977_004760"
     CDS             6449..>7037
                     /gene="narI"
                     /locus_tag="PN977_004760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HDI6044689.1"
                     /translation="MQFLNMFFFDIYPYIAGAVFLIGSWLRYDYGQYTWRAASSQMLD
                     RKGMNLASNLFHIGILGIFVGHFFGMLTPHWMYEAWLPIEVKQKMAMFAGGASGVLCL
                     IGGVLLLKRRLFSPRVRATTTGADILILSLLVIQCALGLLTIPFSAQHMDGSEMMKLV
                     GWAQSVVTFHGGASQHLDGVAFIFRLHLVLGMTLFL"
BASE COUNT         1668 a         1822 c         1967 g         1580 t
ORIGIN      
        1 atttttgatt ttctatatcg cctgcgtggt gattacctgg gcggtatatg gtcggcattc
       61 taaaaaataa atcttaatat ttgattatcc tttgcgcggc atgatgtcgc gcttttttta
      121 tgcgtcattt agttacaaca tactcatatt atattggctt atttcgccgg atttcattaa
      181 gagccattaa tatgttaccc atggggaata ctccttaata cccatctgca taaaaatctt
      241 aatagtttaa ataactacag gtataaaacg tcttaattta cagcgtgtta tgctgtaact
      301 gctaattatc ctaaaggggt atcttaggaa tttactttat ttttcatccc catcactctt
      361 gatcgttatc aattctcacg ctgtttcaga gcgttacttt gcccttaaac attagcaatg
      421 tcgatttatc agagggccga caggctccca caggagaaaa ccgatgagta aattcctgga
      481 ccggtttcgc tacttcaaac agaagggtga aacctttgcc gatgggcatg gccagcttct
      541 caataccaac cgcgactggg aggatggata tcgccagcgt tggcagcatg acaaaatcgt
      601 ccgctctacc cacggggtaa actgcaccgg ctcctgcagc tggaaaatct acgtcaaaaa
      661 cggtctggtg acctgggaaa cccagcagac tgactatccg cgtactcgcc cggacctgcc
      721 aaaccatgaa cctcgcggct gcccgcgcgg tgccagctac tcctggtatc tttacagcgc
      781 caaccgcctg aaatacccaa tgatgcgcaa acgcctgatg aaaatgtggc gtgaagcgaa
      841 ggcgctgcat agcgatccgg ttgaggcatg ggcttctatc attgaagacg ccgataaagc
      901 gaaaagcttt aagcaggcgc gtggacgcgg tggttttgtt cgttcttcct ggcaggaagt
      961 gaatgaactg atcgccgcct ctaacgttta caccatcaaa aactacggcc cggaccgcgt
     1021 cgctggcttc tcgccaattc cggcaatgtc gatggtttct tacgcatcgg gtgcacgcta
     1081 tctttcgctg attggcggta cttgcttaag cttctacgac tggtactgcg acctgcctcc
     1141 tgcgtctccg caaacctggg gcgagcaaac tgacgtaccg gaatctgctg actggtacaa
     1201 ctccagctac atcatcgcct ggggttcaaa cgtgccgcag acgcgtaccc cggatgctca
     1261 cttctttact gaagtgcgtt acaaaggcac caaaactgtt gccgtcacac cagactacgc
     1321 tgaaatcgcc aaactgtgcg atctgtggct ggcaccgaaa cagggcaccg atgcggcaat
     1381 ggcgctggca atgggtcacg taatgctgcg tgaattccac ctcgacaacc caagccagta
     1441 tttcaccgac tatgtgcgtc gctacaccga catgccgatg ctggtgatgc tggaagaacg
     1501 cgacggttac tacgccgcag gtcgtatgct gcgcgctgcc gatctggtgg atgcgctggg
     1561 ccaggaaaac aatccggaat ggaaaactgt cgcctttaat accaatggcg aaatggttgc
     1621 gccgaacggt tctattggct tccgctgggg cgagaagggc aaatggaatc ttgagcagcg
     1681 cgacggcaaa actggcgaag aaaccgagct gcaactgagc ctgctgggta gccaggatga
     1741 gatcgctgag gtgggcttcc cgtactttgg tggcgacggc acggaacact tcaacaaagt
     1801 ggaactggaa aacgtgctgc tgcacaagct gccggtgaaa cgcttgcaac tggctgatgg
     1861 cagcaccgcc ctggtgacca ccgtttatga tctgacgctg gcaaactatg gtctggaacg
     1921 tggcctgaac gacgttaact gtgcagccag ctatgacgat gtgaaagctt acactccggc
     1981 gtgggccgag caaatcactg gcgtttctcg cagccagatt attcgcatcg cccgtgaatt
     2041 tgccgataac gctgataaaa cgcacggtcg ttcgatgatt atcgtcggtg cgggactgaa
     2101 ccactggtat cacctggaca tgaactatcg tggtctgatc aacatgctga ttttctgcgg
     2161 ctgtgtcggt cagagcgggg ggggctgggc gcactatgta ggtcaggaaa aactgcgtcc
     2221 gcaaaccggc tggcagccgc tggcgtttgc cctcgactgg cagcgtccgg cgcgtcacat
     2281 gaacagtacc tcttatttct ataaccactc cagccagtgg cgttatgaaa ccgtgactgc
     2341 ggaagaactg ctgtcgccga tggcggataa atctcgctat accgggcact tgatcgactt
     2401 taacgtccgt gcagaacgca tgggctggct gccgtctgcg ccgcagttag gcactaaccc
     2461 gctgactatc gctcgcgaag cagaaaaagc cgggatgaat ccggtggact atacggtgaa
     2521 atccctgaaa gagggttcta tccgtttcgc tgcggaacag ccggaaaacg gtaaaaacca
     2581 cccgcgtaat ctgttcatct ggcgttctaa cctgctcggt tcttccggta aaggtcatga
     2641 gtttatgctc aagtacctgc tggggacgga gcacggtatc cagggcaaag acctggggca
     2701 gcagggcggc gtgaagccgg aagaagtgga ctggcaggac aacggtctgg aaggcaagct
     2761 ggatctggtg gtgacgctgg acttccgtct gtcgagcacc tgtctctatt ccgacatcat
     2821 tttgccgacg gcgacctggt acgaaaaaga cgacatgaat acttcggata tgcatccgtt
     2881 tattcacccg ctgtctgcgg cggtcgatcc ggcctgggaa gcgaaaagcg actgggaaat
     2941 ctacaaagcc attgcgaaga aattctccga agtgtgcgtt ggtcatctgg gtaaagaaac
     3001 tgacatcgtc acgctgccga tccagcacga ctctgccgct gaactggcgc agccgctgga
     3061 tgtgaaagac tggaaaaaag gcgaatgcga cctgatccca ggcaaaactg caccgcacat
     3121 tatggtcgta gagcgcgatt atccggcaac ttacgaacgc tttacctcta tcggcccgct
     3181 gatggagaaa atcggtaacg gcggtaaagg gattgcctgg aacacccaga gcgagatgga
     3241 tctgctgcgt aagctcaact acaccaaagc ggaaggtccg gcgaaaggcc agccgatgct
     3301 gaacaccgca attgatgcgg cagagatgat cctgacgctg gcaccggaaa ccaacggtca
     3361 ggtagctgta aaagcctggg ctgccctgag cgagtttacc ggccgtgatc atacgcatct
     3421 ggcgctgaat aaagaagacg agaagattcg cttccgcgat attcaggcac agccgcgcaa
     3481 aatcatctcc agcccgacat ggtctggcct ggaagatgaa cacgtttctt acaacgccgg
     3541 ttacaccaac gttcacgagc tgatcccgtg gcgtacgctc tctggccgtc agcagctgta
     3601 tcaggatcac caatggatgc gtgatttcgg tgaaagcctg ctggtttatc gtccgccgat
     3661 cgacacccgt tcggtgaaag aagtgatggg ccagaaatct aacggtaatc cggaaaaagc
     3721 gctcaacttc ctgacaccgc accagaagtg gggtatccac tccacctaca gcgacaacct
     3781 gctgatgctg actttaggtc gcggtggtcc ggtggtctgg ttgagtgaag ccgatgccaa
     3841 agagctgggt atcgccgaca acgactggat tgaagtcttc aacagcaacg gtgcattgac
     3901 tgcccgtgcg gttgtcagcc agcgtgttcc ggcagggatg accatgatgt accacgcgca
     3961 ggaacgtatc gttaacctgc ctggttcgga aattacccaa cagcgtggtg gtatccataa
     4021 ctcggtcacc cgtatcacgc cgaaaccgac gcatatgatc ggcggctatg cccatctggc
     4081 atacggcttt aactactacg gcaccgtagg ttctaaccgc gatgagtttg ttgtagtgcg
     4141 taagatgaag aacattgact ggttagatgg cgaaggcaat gaccaggtac aggagagcgt
     4201 aaaatgaaaa ttcgttcaca agtcggcatg gtgctgaatc tcgataagtg catcggctgc
     4261 cacacctgtt cagttacctg taaaaacgtc tggaccagcc gcgaaggcgt ggaatacgcg
     4321 tggttcaaca acgtggaaac caagccgggc cagggcttcc cgactgactg ggaaaaccag
     4381 gaaaaataca aaggcggctg gatccgtaaa atcaacggca aactgcagcc gcgcatgggt
     4441 aaccgcgcca tgctgctggg taaaatcttc gctaacccgc atctgccggg gatcgacgat
     4501 tattacgagc cgttcgattt tgactatcag aacctgcata ccgcgccgga aggcagcaag
     4561 tcgcagccga ttgcccgtcc gcgttcgctg attaccggcg aacggatggc gaaaatcgaa
     4621 aaagggccga actgggaaga tgacctgggc ggtgagttcg acaagctggc gaaagacaag
     4681 aacttcgaca acatccagaa ggcgatgtat agccagttcg aaaacacctt catgatgtat
     4741 ttgccgcgcc tgtgcgaaca ctgcctgaac ccggcatgtg tggcgacctg cccgagcggt
     4801 gcgatttaca agcgtgaaga agatggcatc gtcctgatcg accaggataa atgccgtggc
     4861 tggcgtatgt gcatcaccgg atgcccgtac aaaaaaatct atttcaactg gaagagcggt
     4921 aagtctgaga agtgcatctt ctgctatccg cgtattgaag cgggtcagcc gaccgtgtgc
     4981 tcagaaacct gtgtcggtcg tatccgttat ctcggcgtgc tgttgtacga tgccgacgct
     5041 attgaacgtg cagccagcac tgagaacgag aaagatcttt accagcgtca gctggacgtg
     5101 ttcctcgatc cgaacgatcc gaaagtcatc gagcaggcga ttaaagacgg tattccgctg
     5161 agcgttattg aagccgcaca gcagtcgccg gtttataaaa tggcgatgga atggaaactg
     5221 gcgctgccgc tgcatccgga atatcgcaca ctgccgatgg tctggtacgt gccgcctctg
     5281 tctccgattc agtctgcagc agacgcgggt gagctgggta gcaacggcat tctgccagac
     5341 gtcgaaagcc tgcgtattcc ggtgcagtat ctggcgaatc tgctgaccgc cggtgatacc
     5401 aaaccggtac tgcgcgcgct gaaacgtatg ctggcgatgc gtcattataa acgtgctgaa
     5461 accgttgacg ggaaagtgga tacccgtgcg ctggaagagg tcggtctgac cgaagcccag
     5521 gcacaggaga tgtaccgtta tctggcgatt gctaactacg aagatcgctt tgtggtgccg
     5581 agtagtcatc gtgaactggc gcgggaagcc ttcccggaga aaaatggctg cggctttacc
     5641 tttggtgatg gctgccacgg ttcagatacc aaattcaatc tgttcaacag ccgccgtatc
     5701 gatgccatcg acgtaaccag caaaacggag ccgcacccat gatcgaactc gtgattgtat
     5761 cgcgtctcct tgaatatccg gatgctgcct tatggcagca tcaacaagag atgtttgagg
     5821 cgattgctgc gtcgaaaaat ctgtcgaaag aagatgccca tgcgctgggc attttcctgc
     5881 gcgatttaac ggcgatggat ccgctcgatg ctcaggccca gtacagcgaa ctgttcgacc
     5941 gtggtcgtgc cacgtcactg ttgctgtttg aacatgtgca cggtgaatcc cgcgaccgcg
     6001 gtcaggcgat ggtagacctg ctggcgcagt atgagcagca cggtttgcag ttaaacagcc
     6061 gcgaattgcc cgaccatctg ccgctgtatc tggagtacct ggcgcagctg ccgcaaagcg
     6121 aagccgtgga gggtttgaaa gatattgcgc cgattctggc attgctgagt gcgcgtctgc
     6181 aacagcgtga aagccgttat gccgtgctgt ttgatctgct gctgaaactg gcgaataccg
     6241 ctatcgacag cgacaaagtg gcggaaaaaa ttgccgacga agcgcgcgac gatacgccgc
     6301 aggcgctgga tgccgtctgg gaagaagagc aggttaaatt ctttgctgac aaaggctgcg
     6361 gtgattcagc aatcactgct catcagcgtc gctttgccgg tgccgtcgcg ccgcaatatc
     6421 tgaatatcac caccggagga cagcactaat gcaattcctg aatatgttct tctttgatat
     6481 ctacccgtac atagccgggg cggtcttcct gattggtagc tggctgcgtt atgactacgg
     6541 gcagtacacc tggcgcgcgg cgtccagcca gatgctggat cgcaaaggga tgaacctggc
     6601 gtcgaacctg ttccatatcg gtattctggg gatttttgtc ggtcacttct tcggtatgct
     6661 gacgccgcac tggatgtatg aagcctggct gccgattgaa gtgaaacaga aaatggcgat
     6721 gtttgctggt ggtgccagcg gtgtgctgtg tctgattggc ggcgtgctgt tgctgaagcg
     6781 tcgtctgttc agcccacgcg tgcgtgcaac cactaccgga gcggatatcc tgatcctgtc
     6841 gctgctcgtt atccagtgcg cgctgggctt gctgaccatt ccgttctcag cccagcatat
     6901 ggacggtagc gagatgatga aactggttgg ctgggcgcag tcggtagtga ccttccacgg
     6961 tggtgcttct caacatcttg atggtgtggc atttatcttc cgtctgcacc tggtgttggg
     7021 gatgacgtta ttcctgc
//
LOCUS       DAMZFT010000203         6900 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.203, whole genome shotgun sequence.
ACCESSION   DAMZFT010000203 DAMZFT010000000
VERSION     DAMZFT010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6900)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6900)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6900
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.203"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..1055
                     /gene="yedE"
                     /locus_tag="PN977_004761"
     CDS             <1..1055
                     /gene="yedE"
                     /locus_tag="PN977_004761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="selenium metabolism membrane protein YedE/FdhT"
                     /protein_id="HDI6044690.1"
                     /translation="LLQLFGVHAEEWGYFKIIHLEGSPLTRIDGMMILGMFGGCFAAA
                     LWANNVKLRMPRSRIRIMQAIIGGIIAGFGARLAMGCNLAAFFTGIPQFSLHAWFFAI
                     ATAIGSWFGARFTLLPIFRIPVKMQKVSAASPLTQKPDQARRRFRLGMLVFFGMLGWA
                     LLTAMNQPKLGLAMLFGVGFGLLIERAQICFTSAFRDMWITGRTHMAKAIIIGMAVSA
                     IGIFSYVQLGVEPKIMWAGPNAVIGGLLFGFGIVLAGGCETGWMYRAVEGQVHYWWVG
                     LGNVIGSTILAYYWDDFAPALATDWDKINLLKTFGPMGGLLVTYLLLFAALMLIIGWE
                     KRFFRRAAPQTAKEIA"
     gene            1052..1285
                     /gene="yedF"
                     /locus_tag="PN977_004762"
     CDS             1052..1285
                     /gene="yedF"
                     /locus_tag="PN977_004762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase-like selenium metabolism
                     protein YedF"
                     /protein_id="HDI6044691.1"
                     /translation="MKNIVPDYRLDMVGEPCPYPAVATLEAMPQLKKGEILEVVSDCP
                     QSINNIPLDARNHGYTVLDIQQDGPTIRYLIQK"
     gene            1394..2062
                     /locus_tag="PN977_004763"
     CDS             1394..2062
                     /locus_tag="PN977_004763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HDI6044692.1"
                     /translation="MCGRFAQSQTREDYLALLAEDIERDIPYDPEPIGRYNVAPGTKV
                     LLLSERDEHLHLDPVFWGYAPGWWDKPPLINARVETAATSRMFKPLWQHGRAICFADG
                     WFEWKKEGDKKQPYFIYRTDGQPVFMAAIGSTPFERGDEAEGFLIVTAAADQGLVDIH
                     DRRPLVLSPDAAREWMRQDIGGKEASEIATSGCVPANQFTWHPVSRAVGNVKNQGAEL
                     IQPV"
     gene            2173..2652
                     /locus_tag="PN977_004764"
     CDS             2173..2652
                     /locus_tag="PN977_004764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_305404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDI6044693.1"
                     /translation="MFTIKTDDLTHPAVQALVAYHISGMLQQSPPESSHALDVQKLRN
                     PTVTFWSVWEGEQLAGIGALKLLDDKHGELKSMRTAPNYLRRGVASLILRHILQVAHD
                     RCLHRLSLETGTQAGFTACHQLYLKHGFVDCEPFADYQLDPHSRFLSLTLCEDNELL"
     gene            complement(2790..3929)
                     /gene="espR3"
                     /locus_tag="PN977_004765"
     CDS             complement(2790..3929)
                     /gene="espR3"
                     /locus_tag="PN977_004765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_305405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector leucine-rich repeat protein EspR3"
                     /protein_id="HDI6044694.1"
                     /translation="MFPLNDLSLKTQSVQLNKVTSNTESTIKQHELVSDDAIINELSS
                     ELVSCLGNGKLTPISEDSNLLNMLSEFKLLREQCFRWGNYTLLFENYGAYDKTGSITI
                     EKSQGEGTLPIRHKLEFISTNIAELLDKLTKITDARLCKGFSDWASSVKEGASNDLKE
                     NVDRALVRMFKCVKLHSNELNLSYLFLGSVPPLPEWIEMLSLIHNKLDSIQVPESCKE
                     LELDFNNLTEFPQVPDGITLISVNNNLISHIDSFPPKVKKIFIGHNKLSEIPAIPDTA
                     KVFDCSENNIKEIRWFPKNLKEVHIEYNKIEVIPAIPGNLKLLFMECNPIKEAFLMPW
                     TLTGICYEISQRKYIVTNPDDYDKYSDMVKKHVIDGEEFIIKYYM"
     gene            complement(4280..4594)
                     /gene="fliE"
                     /locus_tag="PN977_004766"
     CDS             complement(4280..4594)
                     /gene="fliE"
                     /locus_tag="PN977_004766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HDI6044695.1"
                     /translation="MSAIQGIEGVISQLQATAMSARAQESLPQPTISFAGQLHAALDR
                     ISDTQTAARTQAEKFTLGEPGVALNDVMTDMQKASVSMQMGIQVRNKLVAAYQEVMSM
                     QV"
     gene            4809..6467
                     /gene="fliF"
                     /locus_tag="PN977_004767"
     CDS             4809..6467
                     /gene="fliF"
                     /locus_tag="PN977_004767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar M-ring protein FliF"
                     /protein_id="HDI6044696.1"
                     /translation="MNATAAQTKSLEWLNRLRANPKIPLIVAGSAAVAVMVALILWAK
                     APDYRTLFSNLSDQDGGAIVSQLTQMNIPYRFSEASGAIEVPADKVHELRLRLAQQGL
                     PKGGAVGFELLDQEKFGISQFSEQVNYQRALEGELSRTIETIGPVKGARVHLAMPKPS
                     LFVREQKSPSASVTVNLLPGRALDEGQISAIVHLVSSAVAGLPPGNVTLVDQGGHLLT
                     QSNTSGRDLNDAQLKYASDVEGRIQRRIEAILSPIVGNGNIHAQVTAQLDFASKEQTE
                     EQYRPNGDESHAALRSRQLNESEQSGSGYPGGVPGALSNQPAPANNAPISTPPANQNN
                     RQQQASTTSNSGPRSTQRNETSNYEVDRTIRHTKMNVGDVQRLSVAVVVNYKTLPDGK
                     PLPLSNEQMKQIEDLTREAMGFSEKRGDSLNVVNSPFNSSDESGGELPFWQQQAFIDQ
                     LLAAGRWLLVLLVAWLLWRKAVRPQLTRRAEAMKAVQQQAQAREEVEDAVEVRLSKDE
                     QLQQRRANQRLGAEVMSQRIREMSDNDPRVVALVIRQWINNDHE"
     gene            6460..>6900
                     /gene="fliG"
                     /locus_tag="PN977_004768"
     CDS             6460..>6900
                     /gene="fliG"
                     /locus_tag="PN977_004768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310705.1"
                     /note="One of three proteins involved in switching the
                     direction of the flagellar rotation; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HDI6044697.1"
                     /translation="MSNLTGTDKSVILLMTIGEDRAAEVFKHLSQREVQTLSAAMANV
                     TQISNKQLTDVLAEFEQEAEQFAALNINANDYLRSVLVKALGEERAASLLEDILETRD
                     TASGIETLNFMEPQSAADLIRDEHPQIIATILVHLKRAQAADILA"
BASE COUNT         1757 a         1693 c         1683 g         1767 t
ORIGIN      
        1 agctcctgca actgttcggc gtccatgctg aagagtgggg ttactttaaa attatccatc
       61 tggaaggatc gccattaacc cgcatcgacg ggatgatgat cctcggtatg tttggcggct
      121 gctttgccgc agcgctgtgg gccaacaatg tcaaactgcg aatgccgcgc agccgtatcc
      181 gcattatgca ggccatcatt ggcggcatta tcgccggttt tggcgcgcgt ctggcaatgg
      241 gctgtaacct ggcggcgttc tttaccggta ttcctcagtt ctcgctgcat gcctggttct
      301 ttgccatcgc cactgccatt ggttcatggt ttggcgcgcg ctttaccctg ctgcccatct
      361 tccgtattcc cgtgaaaatg cagaaagttt ctgccgcctc accgctgacg caaaaaccgg
      421 atcaggcgcg gcgtcgtttt cgtctcggga tgctggtctt tttcggcatg ctgggctggg
      481 cgctgctcac agcgatgaac caacccaaac tggggctggc aatgctgttt ggcgtcggct
      541 ttggtttact gattgaacgt gcgcaaatct gctttacttc ggcgttccgc gatatgtgga
      601 tcaccggacg tacccatatg gcgaaagcaa tcattattgg tatggcggtg agtgccatcg
      661 ggatcttcag ttacgtacag ttaggcgttg aacccaaaat catgtgggcg ggaccaaacg
      721 cggtaattgg tggtttactg tttggttttg gcatcgtgct ggctggcggc tgcgaaaccg
      781 gctggatgta ccgcgcggta gaaggccagg tgcactactg gtgggtcggt ctgggcaatg
      841 tgatcggctc aacgattctg gcgtattact gggatgattt cgctccggcg ctggccaccg
      901 actgggacaa aatcaacctg ctgaaaacct ttggtccgat ggggggcctg ctggtgacat
      961 atttgctgtt gtttgctgcg ctgatgttga ttatcggctg ggaaaaacgc ttcttccgcc
     1021 gtgcggcacc gcagactgct aaggagatcg catgaaaaat atcgttcctg attaccgcct
     1081 tgatatggtg ggtgaaccct gcccttatcc ggcagtcgca acccttgagg cgatgccgca
     1141 gttaaaaaaa ggggaaatcc tggaagtggt gagcgactgt ccgcagtcga tcaataatat
     1201 tccactggat gcgcgtaatc acggctatac ggtgctggat attcagcaag acgggccgac
     1261 cattcgttat ttaattcaga agtaatccct cattcccgct gtgacccaca gcgggaacat
     1321 cgttaccttt gtatcacata gattttaccc accccctctc ctattctttt cagtatgatc
     1381 tcaggaggtt tctatgtgtg gacgctttgc ccaatcccaa acgcgtgaag attaccttgc
     1441 gcttctcgcg gaagatattg aacgcgatat tccctatgat cccgaaccca ttggcagata
     1501 caacgtcgcg ccgggaacca aagtcctact gctcagtgaa cgtgatgaac accttcatct
     1561 ggatccggtt ttctggggat atgctcccgg atggtgggat aaaccgccgc tgattaacgc
     1621 ccgcgtagaa actgcggcga ccagtcgtat gtttaaaccg ctctggcaac atggtcgggc
     1681 aatctgtttt gccgatggct ggtttgagtg gaaaaaagaa ggcgacaaaa agcagcctta
     1741 ttttatctat cgtactgatg gacaacctgt ttttatggcc gcgataggca gcacaccatt
     1801 cgagcgcggt gacgaagccg aaggattttt gattgtcact gcggcggcag atcaaggtct
     1861 ggtggatatt catgaccgcc gcccactggt actctcgcca gatgctgcgc gggaatggat
     1921 gcggcaagat attggcggta aagaagcctc agaaatcgct accagtggtt gtgtgccagc
     1981 aaaccagttc acctggcacc cggtatcgcg cgcggtgggt aatgttaaaa accagggcgc
     2041 ggagttaatt caacctgttt gataagcctg gagattattg atcaacaata ctgcgtcata
     2101 agtaatctct attagacaaa gatttcatta cctgttggca tattgcaaaa ataacaccaa
     2161 tacggaatcg tcatgttcac gattaaaaca gatgatctca cccatccagc agtgcaagca
     2221 ttagtggctt accatatttc cggcatgctg cagcagtctc cccctgaaag cagtcatgct
     2281 ttagatgtgc aaaaattacg taacccgaca gtgacattct ggtcagtatg ggaaggcgaa
     2341 caactcgcag gaattggtgc gctgaagttg ctggatgata aacatggcga actgaaatca
     2401 atgcgtaccg cgccaaatta tttacgtcgg ggtgtcgcca gtctgatttt acgccacatt
     2461 ttgcaggtcg cccatgacag atgccttcat cgcctgagct tagaaacggg tacacaggct
     2521 ggatttacgg cctgccatca actttatttg aagcatggtt tcgttgattg cgaaccgttt
     2581 gccgattatc aacttgatcc acacagtcga tttttgtcat tgacgctatg cgaagataat
     2641 gagttgcttt gagccagacg cagcacattc ttgcattcga cgtgctgcgt ctttatttat
     2701 caccagcagg aaacgccttg tccatagacg ccccttccac atgcgtcaca agaaacctct
     2761 attccagtga cacaattacg cctaattaat tacatataat atttaattat gaattcctca
     2821 ccatctatta catgcttttt aaccatatcg gaatatttat cataatcatc ggggttcgta
     2881 acaatatatt tccgctgcga tatttcatag caaatccctg tcagagtcca tggcattaaa
     2941 aatgcctctt taataggatt acattccata aaaagtaatt ttaaattgcc aggtatcgca
     3001 ggaataacct caatcttatt atattcaata tgcacttctt tcagattttt ggggaaccac
     3061 ctaatttctt ttatattatt ctcactacaa tcaaaaacct tagcggtgtc tggtattgct
     3121 ggtatttccg atagcttatt gtgaccaata aaaattttct taacctttgg cggaaatgag
     3181 tcaatatgcg atatcaggtt attatttacg gagatcaggg taattccatc aggtacttgt
     3241 ggaaattctg taaggttatt gaaatcgagt tctaattctt tgcatgattc gggcacctgt
     3301 attgaatcaa gtttattatg aataaggcta agcatttcaa tccactctgg aagaggcggc
     3361 acagaaccca aaaaaaggta tgataagtta agttcattac tgtggagctt aacacattta
     3421 aacattctca ccaatgctct atccacattt tctttcaagt cattcgatgc gccttctttg
     3481 actgaactag cccagtcact gaaacctttg caaagtctgg catctgtaat tttggttaac
     3541 ttgtccagca actctgcaat attagtcgat ataaactcta atttatgccg aatgggtaaa
     3601 gtcccctccc cctgactttt ttctatcgtg atagatcccg tcttatcata agccccataa
     3661 ttttcaaaca atagagtata attaccccac ctgaaacatt gctctctcaa taacttaaat
     3721 tcagacagca tattcagtaa gttgctgtct tcactaattg gtgtaagctt accatttcca
     3781 agacaactga ctaactcact tgataattca tttatgattg catcatcaga tactaactcg
     3841 tgttgtttta tcgtagattc agtattagat gtgactttat ttaattgaac cgactgagtt
     3901 tttaacgaaa gatcatttaa tggaaacatt tacactcctg tttaataaat tatattgccc
     3961 agggcgcatt ctaaaacaca ataaaattac cactatcaaa aatcgctcat atccatagcc
     4021 cggaatatga ataaaaacat caacaaagat taaattatcc agaggttact gttgcgtctg
     4081 tggtcgtaac aacatccgac gtggtgatgt gtgtcaaatg agacattctg atgatcagag
     4141 ggtttatcgt gtatacggat cgtggatggc agaaaacttt caggatcagg tatccatttc
     4201 aaaccagata atgagtaagt ttgccccatc catgccccac gcggtgagat cggatgtcat
     4261 taacaacagg ttacacaacc tatacctgca tgctcattac ttcctgatat gccgccacca
     4321 gcttattacg cacctgaatc cccatttgca ttgaaactga ggctttttgc atatcggtca
     4381 tcacatcgtt taacgccacg ccgggttcac cgagagtgaa tttttctgcc tgcgtgcggg
     4441 cggctgtttg tgtatcgctt atgcgatcga gcgcggcgtg cagctgcccg gcaaaactaa
     4501 tggtcggttg cggcagtgat tcctgcgcac gcgcactcat cgccgtagcc tgtaactggc
     4561 tgataacccc ttcaatcccc tgtatcgctg acattttcat ctcctggata atttctggta
     4621 gcaaagccta ccagtaagtc aataagacaa aggcgctaaa tagcaacaaa aaaacgggtt
     4681 tattggcgga tagaaaaaaa cgaaagcaca aataatggga gcgtcaattt ttcgagtttg
     4741 ctgacccggg agtgagtctt gttccacttt gccaataacg ccgtccataa tcagccacga
     4801 ggtgcgcgat gaatgcgact gcagcccaga caaaatctct tgagtggctt aatcgcctgc
     4861 gtgcgaatcc gaaaattcca ttgattgttg ccggttccgc ggcagtggcg gtcatggtcg
     4921 cactgatcct gtgggcgaaa gcccccgact accgcacatt attcagcaat ctttccgatc
     4981 aggatggtgg cgcaattgtc agccaactga cgcaaatgaa tattccttac cgcttcagcg
     5041 aagccagcgg cgctattgaa gttccggcag ataaagttca cgaactgcgt ctgcgcctgg
     5101 cacaacaagg tttgcccaaa ggcggcgcgg tcggtttcga actactcgat caggaaaagt
     5161 ttggtatcag ccagttcagc gaacaggtga attatcagcg ggcgctggaa ggcgagcttt
     5221 ctcgtaccat cgaaactatc ggcccggtaa aaggggcgcg cgtacatctg gcaatgccga
     5281 aaccgtcttt attcgtccgt gaacaaaaat ccccttctgc atcggtgacg gtaaatctgt
     5341 tacccggccg cgcactcgat gaagggcaaa ttagcgccat tgtgcatctg gtttccagcg
     5401 ccgttgctgg tctgccgccg ggaaacgtca cgctggtgga tcagggcgga catctgttaa
     5461 cccagtccaa taccagcggg cgcgatctta atgatgctca gttgaaatat gccagcgatg
     5521 tcgaaggccg tattcagcgg cgtattgaag caatcctgtc gcctattgtt ggtaacggta
     5581 atattcacgc ccaggtcacg gcgcagctgg acttcgccag taaagaacaa acggaagaac
     5641 agtatcgccc taacggtgat gaatctcatg cggcgcttcg ttcacgccag cttaatgaga
     5701 gcgagcaaag cggttccggt tatccgggcg gcgtaccggg ggcgttgtcg aatcaaccgg
     5761 cacctgcgaa taacgcgcca atcagcacgc ctccggcaaa tcaaaataac cgccagcagc
     5821 aggcgagcac caccagcaat agcgggccgc gtagcacaca gcggaatgaa accagtaact
     5881 acgaagtcga tcgcaccatt cgtcatacca aaatgaacgt gggcgatgtg caacgtctgt
     5941 cagtcgcggt agtggtgaat tacaaaacct tgccagacgg taaaccattg cctctcagca
     6001 acgaacagat gaagcaaatt gaagatctga cccgcgaggc gatgggcttt tctgaaaaac
     6061 gcggtgactc gctcaatgtc gttaactcgc cgttcaatag cagtgacgaa agcggcggag
     6121 aactgccgtt ctggcaacag caagcgttta tcgatcagtt gctggctgcc ggtcgctggt
     6181 tgctggtact gctggtggcg tggctgctgt ggcggaaagc ggtacgcccg cagttaacac
     6241 gtcgcgccga ggcgatgaaa gctgtacagc aacaggcgca ggcccgtgag gaagtggaag
     6301 atgcggtgga agtccgcctg agcaaagacg aacaactcca acaacgacgc gctaaccaac
     6361 gtctgggggc agaagtcatg agccagcgta tccgtgaaat gtctgataac gatccgcgcg
     6421 tggtggcgct ggtcattcgc cagtggataa ataacgatca tgagtaacct gacaggcacc
     6481 gataaaagcg tcatcctgct gatgaccatt ggcgaagacc gggcggcaga ggtgttcaag
     6541 cacctctccc agcgcgaagt gcaaaccctg agcgctgcaa tggcgaacgt cacgcagatc
     6601 tccaacaagc agctaaccga tgtgctggcg gagtttgagc aagaagctga acagtttgcc
     6661 gcactgaata tcaacgccaa cgattatctg cgttcggtat tggtcaaagc tctgggtgag
     6721 gaacgtgccg ccagcctgct ggaagatatt ctcgaaactc gcgataccgc cagcggtatt
     6781 gaaacgctca actttatgga gccgcagagt gccgccgatc tgattcgcga tgagcatccg
     6841 caaattatcg ccaccattct ggtgcatctg aagcgcgccc aggccgccga tattctggcg
//
LOCUS       DAMZFT010000204         6834 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.204, whole genome shotgun sequence.
ACCESSION   DAMZFT010000204 DAMZFT010000000
VERSION     DAMZFT010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6834)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6834)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6834
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.204"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(164..955)
                     /gene="cpoB"
                     /locus_tag="PN977_004769"
     CDS             complement(164..955)
                     /gene="cpoB"
                     /locus_tag="PN977_004769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein CpoB"
                     /protein_id="HDI6044698.1"
                     /translation="MSSNYRHQLLSLSLLVGIAAPWAAFAQAPISSVGSGSVEDRVTQ
                     LERISNAHSQLLTQLQQQLSDNQSDIDSLRGQIQENQYQLNQVVERQKQILLQIDSLS
                     SGGAAAQSTSGDQSGAAASTTPTADAGTANAGAPVKSGDANTDYNAAIALVQDKSRQD
                     DAMVAFQNFIKNYPDSTYLPNANYWLGQLNYNKGKKDDAAYYFASVVKNYPKSPKAAD
                     AMFKVGVIMQDKGDTAKAKAVYQQVISKYPGTDGAKQAQKRLNAM"
     gene            complement(965..1486)
                     /gene="pal"
                     /locus_tag="PN977_004770"
     CDS             complement(965..1486)
                     /gene="pal"
                     /locus_tag="PN977_004770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HDI6044699.1"
                     /translation="MQLNKVLKGLMIALPVMAIAACSSNKNASNDGSEGMLGAGTGMD
                     ANGGNGNMSSEEQARLQMQQLQQNNIVYFDLDKYDIRSDFAQMLDAHANFLRSNPSYK
                     VTVEGHADERGTPEYNISLGERRANAVKMYLQGKGVSADQISIVSYGKEKPAVLGHDE
                     AAYSKNRRAVLVY"
     gene            complement(1521..2813)
                     /gene="tolB"
                     /locus_tag="PN977_004771"
     CDS             complement(1521..2813)
                     /gene="tolB"
                     /locus_tag="PN977_004771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001646851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolB"
                     /protein_id="HDI6044700.1"
                     /translation="MKQALRVAFGFLILWASVLHAEVRIVIDSGVDSGRPIGVVPFQW
                     AGPGAAPEDIGGIVAADLRNSGKFNPLDRARLPQQPGSAQEVQPAAWSALGIDAVVVG
                     QVTPNPDGSYNVAYQLVDTGGAPGTVLAQNSYKVNKQWLRYAGHTASDEVFEKLTGIK
                     GAFRTRIAYVVQTNGGQFPYELRVSDYDGYNQFVVHRSPQPLMSPAWSPDGSKLAYVT
                     FESGRSALVIQTLANGAVRQVASFPRHNGAPAFSPDGSKLAFALSKTGSLNLYVMDLA
                     SGQIRQVTDGRSNNTEPTWFPDSQNLAFTSDQAGRPQVYKVNINGGAPQRITWEGSQN
                     QDADVSSDGKFMVMVSSNGGQQHIAKQDLATGGVQVLSSTFLDETPSLAPNGTMVIYS
                     SSQGMGSVLNLVSTDGRFKARLPATDGQVKFPAWSPYL"
     gene            complement(2946..4226)
                     /gene="tolA"
                     /locus_tag="PN977_004772"
     CDS             complement(2946..4226)
                     /gene="tolA"
                     /locus_tag="PN977_004772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="HDI6044701.1"
                     /translation="MSKATEQNDKLKRAIIISAVLHVILFAALIWSSFDENIEASAGG
                     GGGSSIDAVMVDSGAVVEQYKRMQSQESSAKRSDEQRKMKEQQAAEELREKQAAEQER
                     LKQLEKERLAAQEQKKQAEEAAKQAELKQKQAEEAAAKAAADAKAKAEADAKAAEEAA
                     KKAAADAKKKAEAEAAKAAAEAQKKAEAAAAALKKKAEAAEAAAAEARKKAATEAAEK
                     AKAEAEKKAAAEKAAADKKAAAEKAAADKKAAEKAAAEKAAADKKAAAEKAAADKKAA
                     AAKAAAAKAAAEKAAAAKAAAEADDIFGELSSGKNAPKTGGGAKGNNASPAGSGNTKN
                     NGASGADINNYAGQIKSAIESKFYDASSYAGKTCTLRIKLAPDGMLLDIKPEGGDPAL
                     CQAALAAAKLAKIPKPPSQAVYEVFKNAPLDFKP"
     gene            complement(4291..4719)
                     /gene="tolR"
                     /locus_tag="PN977_004773"
     CDS             complement(4291..4719)
                     /gene="tolR"
                     /locus_tag="PN977_004773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin uptake protein TolR"
                     /protein_id="HDI6044702.1"
                     /translation="MARARGRGRRDLKSEINIVPLLDVLLVLLLIFMATAPIITQSVE
                     VDLPDATESQAVSSNDNPPVIVEVSGIGQYTVVVEKDRLERLPPEQVVAEVSSRFKAN
                     PKTVFLIGGAKDVPYDEIIKALNLLHSAGVKSVGLMTQPI"
     gene            complement(4723..5415)
                     /gene="tolQ"
                     /locus_tag="PN977_004774"
     CDS             complement(4723..5415)
                     /gene="tolQ"
                     /locus_tag="PN977_004774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolQ"
                     /protein_id="HDI6044703.1"
                     /translation="MTDMNILDLFLKASLLVKLIMLILIGFSIASWAIIIQRTRILNA
                     AAREAEAFEDKFWSGIELSRLYQESQGKRDNLTGSEQIFYSGFKEFVRLHRANSHAPE
                     AVVEGASRAMRISMNRELENLETHIPFLGTVGSISPYIGLFGTVWGIMHAFIALGAVK
                     QATLQMVAPGIAEALIATAIGLFAAIPAVMAYNRLNQRVNKLELNYDNFMEEFTAILH
                     RQAFTVSESNKG"
     gene            complement(5412..5816)
                     /gene="ybgC"
                     /locus_tag="PN977_004775"
     CDS             complement(5412..5816)
                     /gene="ybgC"
                     /locus_tag="PN977_004775"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HDI6044704.1"
                     /translation="MNTTLFRWPVRVYYEDTDAGGVVYHASYVAFYERARTEMLRHHH
                     FSQQALMAERVAFVVRKMTVEYYAPARLDDMLEIQTEITSMRGTSLVFTQRIVNAENT
                     LLNEAEVLVVCVDPLKMKPRALPKSIVAEFKQ"
     gene            complement(5966..6259)
                     /gene="ybgE"
                     /locus_tag="PN977_004776"
     CDS             complement(5966..6259)
                     /gene="ybgE"
                     /locus_tag="PN977_004776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyd operon protein YbgE"
                     /protein_id="HDI6044705.1"
                     /translation="MSKIIATLYAVMDKRPLRALSFVMALLLAGCMFWDPSRFAAKTS
                     ELEIWHGLLLMWAVCAGVIHGVGFRPQKVLWQGIFCPLLADIVLIVGLIFFFF"
     gene            complement(6259..6372)
                     /gene="cydX"
                     /locus_tag="PN977_004777"
     CDS             complement(6259..6372)
                     /gene="cydX"
                     /locus_tag="PN977_004777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-I oxidase subunit CydX"
                     /protein_id="HDI6044706.1"
                     /translation="MWYFAWILGTLLACSFGVITALALEHVESGKAGQEDI"
     gene            complement(6387..>6834)
                     /gene="cydB"
                     /locus_tag="PN977_004778"
     CDS             complement(6387..>6834)
                     /gene="cydB"
                     /locus_tag="PN977_004778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HDI6044707.1"
                     /translation="KSTMDHYAASNPLNKEVVREAGAWLVNFNNTPILWAIPALGVVL
                     PLLTILTARMDKAAWAFVFSSLTLACIILTAGIAMFPFVMPSSTMMNASLTMWDATSS
                     QLTLNVMTWVAVVLVPIILLYTAWCYWKMFGRITKEDIERNTHSLY"
BASE COUNT         1571 a         1860 c         1622 g         1781 t
ORIGIN      
        1 ctttttcgtt gctttcggtt tgtttgatat cccgtggcaa cggcggcata tattactgat
       61 ttcagacttg agcgcaacaa aaatttcgat gcagatcact caactgctta tgattcgcac
      121 gacacgacca gaaataatgc gacttctggt cgtgcgttat gcattacatc gcgttcagac
      181 gtttttgcgc ctgtttagcg ccatcagtac cagggtattt actgataacc tgctggtata
      241 cggctttcgc ttttgcggtg tcacctttgt cctgcatgat gacgccgact ttaaacatcg
      301 catctgcagc cttcggtgac ttcggatagt ttttcactac cgaagcaaaa taatacgccg
      361 catcatcttt tttacccttg ttgtagttta actgacccag ccaataattg gcgtttggca
      421 ggtaagttga atcagggtaa tttttgatga aattctgaaa tgccaccatt gcgtcatcct
      481 ggcgggattt atcctgcacc agcgcaatag ctgcattgta atccgtgttt gcatcaccgc
      541 ttttcaccgg cgcgccagca ttcgcagtac cagcatcagc tgtcggcgtc gttgatgccg
      601 ccgcaccgct ttgatcaccg ctggttgatt gcgccgctgc accaccgctg ctgaggctgt
      661 cgatctgcaa caggatctgc ttttgccgct ccacgacctg attcagttga tactgatttt
      721 cctgaatctg accgcgcagg gaatcaatat cggattgatt atcagagagt tgttgctgga
      781 gttgggttaa aagctggctg tgagcatttg aaatacgctc aagttgagtg acgcggtctt
      841 cgaccgagcc tgagccgaca ctactgattg gtgcctgagc aaaagcggcc cagggggccg
      901 ctataccaac cagtaacgac agactcaata gttgatgtct gtagttactg ctcatgcaat
      961 tctcttagta aaccagtacc gcacgacggt ttttggagta tgccgcttcg tcatgaccca
     1021 gtactgcagg tttttcttta ccgtaagaaa cgatggagat ctggtctgca gaaacgcctt
     1081 taccctgcag gtacatctta acggcgttcg cacgacgttc acccagggag atgttgtatt
     1141 ccggagtacc acgttcgtcc gcgtgacctt ctacggtgac tttgtaagac gggttgctac
     1201 gcaggaagtt tgcgtgtgca tccagcattt gagcgaagtc agaacggata tcgtacttgt
     1261 ccagatcgaa gtaaacgatg ttgttctgct gcagctgttg catttgcaga cgagcctgct
     1321 cttcggaaga catgttgccg ttgccgccgt tcgcatccat accagtgccg gcacccagca
     1381 tgccttcgct gccgtcattg ctggcgttct tgttggaaga acatgccgca attgccataa
     1441 caggcagagc aatcatcagc cctttcagca ctttgttcag ttgcatttca atgattcctt
     1501 tactattcaa ttaattatta tcacagatac ggcgaccagg cagggaattt gacctgtcca
     1561 tcagttgccg gaagacgcgc tttgaaacgc ccatctgtag aaaccaaatt cagcacggat
     1621 cccatcccct gagaagagct gtagattacc atagtgccgt taggtgccag acttggcgtt
     1681 tcatccagga acgtggacga cagaacttgt acgcctcccg ttgccagatc ttgtttggca
     1741 atgtgctgct gcccaccatt ggagctgacc attaccataa atttaccgtc gctgctgaca
     1801 tccgcatcct ggttctgcga accttcccag gtaatacgtt gtggcgcacc gccgttgata
     1861 ttcactttat aaacctgtgg acgaccggcc tggtcagaag taaatgccag gttctggcta
     1921 tccgggaacc aggtcggttc ggtattgtta ctgcgaccat cagtcacctg gcggatctga
     1981 ccagaagcca aatccattac gtacaggttc agactaccgg ttttcgacaa ggcgaatgcc
     2041 agtttgctgc cgtctggcga gaatgcaggt gcaccgttgt gacgcgggaa tgaagccacc
     2101 tggcgtacag cgccatttgc cagcgtctga ataaccagcg cggaacgacc gctttcgaag
     2161 gtcacataag ccagtttaga accgtctggt gaccacgccg gagacatcag cggctgcggt
     2221 gaacggtgaa cgacaaactg gttgtaaccg tcatagtcag atacgcgcag ttcatacggg
     2281 aactgaccgc cgttggtctg aacaacgtag gcaatacggg tacggaacgc acctttaatg
     2341 ccggtcagtt tttcaaacac ttcatcactg gcggtatgac cagcataacg cagccactgc
     2401 ttgttcactt tgtacgagtt ctgagcaagt acagtacccg gtgcgccgcc agtgtcaaca
     2461 agttgataag caacattgta agagccgtcc ggattcggag tgacctgacc gacaactaca
     2521 gcgtcaatgc ccagtgcgga ccatgcagct ggttgtactt cctgcgcact acccggctgc
     2581 tgtggcagac gagcgcgatc taacggatta aatttaccgc tgttacgcaa gtcagcagca
     2641 acgatgccgc caatatcttc aggtgccgca ccaggccccg cccactggaa aggaacaaca
     2701 ccaataggac gaccggaatc tacaccgctg tcgatcacaa tgcggacttc agcatgcaga
     2761 actgatgccc acagtatgag aaaaccaaat gctactcgta atgcctgctt catcatatct
     2821 cccttatctg gaccgatggc ccacgataat ttagcagaat gttaacaaac taaaatacac
     2881 aaaactacca gaaccccgtg gcaaccggtg cctgatgttg accgtccgaa cagtcaacat
     2941 cgcgattacg gtttgaagtc caatggcgcg tttttgaaca cttcatatac tgcctggctt
     3001 ggtggtttcg ggatcttcgc aagtttagct gctgctaacg cagcctgaca aagtgcggga
     3061 tcgccacctt caggtttgat atccagtaac atgccatcgg gtgccagttt tatgcgcagc
     3121 gtacaggttt tgcctgcata ggacgatgcg tcatagaact tactttcgat agcagattta
     3181 atctgcccgg catagttatt gatatcggcc cctgatgcgc cattgttttt agtattacca
     3241 ctcccggctg gcgaagcatt gttccctttc gcccctcccc ccgttttcgg tgcattctta
     3301 ccagagctta gctcaccgaa aatatcatct gcctctgcgg cagcttttgc tgcagcggct
     3361 ttttcagctg ctgcttttgc agctgccgct tttgcagctg ccgctttttt gtctgcggcg
     3421 gctttttctg ccgctgcttt cttatcagct gctgcctttt cagcagccgc tttttctgct
     3481 gcttttttgt cggctgcagc tttctctgct gccgctttct tatcagctgc agccttttca
     3541 gcagccgctt tcttctcagc ttctgctttg gctttttcag cagcttcagt tgccgctttc
     3601 tttcttgctt cagctgcagc tgcttctgcc gcttccgctt tcttcttcag tgccgccgcg
     3661 gctgcctcgg cttttttctg cgcttcggct gcggctttgg cggcttctgc ttctgctttt
     3721 ttcttcgcgt ctgcagccgc tttcttcgct gcttcttccg cagcttttgc atccgcttca
     3781 gccttcgctt tagcatctgc cgccgctttc gctgccgctt cttccgcttg cttctgcttt
     3841 aactcggcct gttttgcggc ttcttcagcc tgctttttct gttcctgagc cgctaaccgc
     3901 tctttctcaa gttgcttcag gcgttcctgt tcagccgctt gtttctcacg cagttcttca
     3961 gcagcctgct gttccttcat cttgcgctgc tcatcagaac gcttcgcgct tgattcctgg
     4021 ctttgcatgc gtttgtactg ctcaactacc gcacctgaat caaccatgac agcgtcgatg
     4081 gacgaaccac cgccgcctcc ggctgaagct tctatattct catcgaacga actccagatc
     4141 agcgccgcaa ataagatgac atgcagcact gctgaaataa ttatcgcccg tttgagcttg
     4201 tcgttttgtt cggttgcctt tgacactctc ggtttccaaa aactgttcgc ctgttacccg
     4261 ctctctttca agcaagggaa acgcagatgt ttagataggc tgcgtcatta aaccaaccga
     4321 tttcacaccc gcactatgta acaagttcag tgctttaatt atttcatcgt aaggcacatc
     4381 ttttgcgcca ccgatcagaa agaccgtttt cgggttggcc ttgaaacggc tggacacttc
     4441 cgccaccacc tgctctggtg gtaaacgctc caggcgatct ttctcaacca ccacggtgta
     4501 ctgaccaata ccagacactt caacaatcac tggcggatta tcgttactgc tcaccgcctg
     4561 tgattcagta gcgtctggca gatcgacctc cacgctctgg gtgatgatgg gcgctgtcgc
     4621 cataaagatc agcaacagca ccagcagtac gtccagcaac ggtacaatgt tgatttcgga
     4681 cttgagatcg cgacgacctc gtccacgcgc tctggccatg gcttacccct tgttgctctc
     4741 gctaacggta aacgcctggc ggtgcagaat cgcggtaaac tcttccataa agttgtcgta
     4801 attcagttcc agtttgttta cgcgctggtt gaggcggtta taagccatta ccgccggaat
     4861 tgcggcgaac agaccaattg ccgtcgcaat caacgcttct gcgataccgg gcgcaaccat
     4921 ttgcagtgtc gcttgtttta ctgccccgag ggcgataaag gcgtgcatga tcccccagac
     4981 cgtaccaaac agaccaatat acgggctgat ggagccaacc gtaccgagga acggaatgtg
     5041 cgtttccaga ttttcaagtt cacggttcat ggagatacgc atagcacgcg acgccccttc
     5101 cactacggct tccggcgcat ggctattggc acgatgcagg cgcacaaact ctttgaaccc
     5161 gctgtaaaag atttgttccg aaccagtcag attgtcccgt ttcccctgac tctcttgata
     5221 gaggcgagag agttcgattc cagaccagaa tttatcttca aacgcttcgg cttcacgcgc
     5281 cgctgcgtta agaatacggg tccgctggat aataatggcc caggatgcga ttgaaaaacc
     5341 aatcaaaatc aacatgataa gtttaaccag aaggctagcc ttcaggaaca aatcaaggat
     5401 attcatgtca gtcactgctt aaactccgcg acaatagact tgggaagcgc acgaggcttc
     5461 attttgagtg ggtcaacgca aacaaccaga acctctgctt cattcagcaa agtattctcg
     5521 gcgttgacaa tacgttgcgt gaaaaccaaa gaggtgccac gcattgatgt tatttcagtc
     5581 tgtatttcga gcatatcgtc gagccgcgca ggtgcgtaat attccaccgt cattttacgt
     5641 accacaaagg caacgcgttc agccatcagc gcctgttgac tgaagtgatg atgacgcagc
     5701 atctctgtgc gtgctctttc ataaaaagcg acgtaactgg cgtggtacac cacaccaccg
     5761 gcatcggtat cttcatagta gacgcgaacc ggccatcgaa acagcgttgt attcacttta
     5821 catcccggta atgcaacaaa agttagagct tttaaacgct gctactatac gcgcgcgatt
     5881 gaggtttggg aatgagggca agttaaggga gcgtaaaaat ttatgggcct gaagcggccc
     5941 ataaattttt aaagagaatt ctgatttaaa agaagaagaa aatcagcccg acaatgagaa
     6001 caatatcggc aagcaaaggg caaaaaatcc cttgccagag aaccttctgc ggacgaaagc
     6061 ccacgccgtg aatcacacca gcacagacgg cccacatcag caataaacca tgccagattt
     6121 ccagttcact ggtcttcgcg gcgaaacgtg acgggtccca aaacatacat cctgctaaca
     6181 gaagcgccat cacgaaggaa agcgcccgca gggggcgctt gtccattacc gcatacaagg
     6241 ttgcgataat cttactcatc agatgtcttc ttgaccggct ttgcctgatt cgacgtgctc
     6301 aagcgccagc gcggtgatta ccccaaacga acaggcaaga agcgttccca gaatccatgc
     6361 gaaataccac atttttagct ccttacttag tacagagagt gggtgttacg ttcaatatct
     6421 tctttggtga tacgaccgaa cattttccag taacaccagg cggtgtagag cagaatgatc
     6481 ggtaccagaa ccaccgcaac ccaggtcatg acgttaagcg tcagctggct ggaagttgca
     6541 tcccacattg tcagacttgc gttcatcatg gtgctggacg gcatcacaaa cgggaacatt
     6601 gcgataccgg cagtcaggat gatgcaggcc agcgtcaggg aggagaacac aaacgcccac
     6661 gcggctttat ccatacgtgc agtcaggatg gtcagcagcg gcagaaccac acccagtgcc
     6721 ggaatagccc acagaattgg cgtgttgttg aagttaacca gccatgcgcc agcttcacga
     6781 accacttctt tattcagcgg gttagaggct gcgtaatggt ccattgtcga tttc
//
LOCUS       DAMZFT010000205         6826 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.205, whole genome shotgun sequence.
ACCESSION   DAMZFT010000205 DAMZFT010000000
VERSION     DAMZFT010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6826)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6826)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6826
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.205"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..829
                     /gene="dhaK"
                     /locus_tag="PN977_004779"
     CDS             <1..829
                     /gene="dhaK"
                     /locus_tag="PN977_004779"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309732.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HDI6044708.1"
                     /translation="TPDKIFECAMQIDGGEGVLLIIKNYTGDILNFETATELLHDSGV
                     KVTTVVIDDDVAVKDSLYTAGRRGVANTVLIEKLVGAAAERGDSLDACAELGRKLNNQ
                     GHSIGIALGACTVPAAGKPSFTLADNEMEFGVGIHGEPGIDRRPFSSLDQTVDEMFDT
                     LLENGSYHRTLRFWDYQQGSWQEEPQTKQPLQSGDRVIALVNNLGATPLSELYGVYNR
                     LTTRCQQAGLTIERNLIGAYCTSLDMTGFSITLLKVDDETLALWDAPVHTPALNWGK"
     gene            840..1472
                     /gene="dhaL"
                     /locus_tag="PN977_004780"
     CDS             840..1472
                     /gene="dhaL"
                     /locus_tag="PN977_004780"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase ADP-binding subunit
                     DhaL"
                     /protein_id="HDI6044709.1"
                     /translation="MSLSRTQIVNWLTRCGDIFSTESEYLTGLDREIGDADHGLNMNR
                     GFSKVVEKLPAIADKDIGFILKNTGMTLLSSVGGASGPLFGTFFIRAAQATQARQSLT
                     LEELYQMFRDGADGVISRGKAEPGDKTMCDVWVSVVESLRQSCEQNLSVPAALDTASS
                     IAESAAQSTITMQARKGRASYLGERSIGHQDPGATSVMFMMQMLALAAKE"
     gene            1483..2901
                     /gene="dhaM"
                     /locus_tag="PN977_004781"
     CDS             1483..2901
                     /gene="dhaM"
                     /locus_tag="PN977_004781"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415716.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaM"
                     /protein_id="HDI6044710.1"
                     /translation="MVNLVIVSHSSRLGEGVGELARQMLMGDICKIAIAAGIDDPQNP
                     IGTDAVKVMEAIESVADADHVLVMMDMGSALLSAETALELLAPEIAAKVRLCAAPLVE
                     GTLAATVSAASGADIDKVIFDAMHALEAKREQLGLPSSDTEISDTCPPYDEEARSLSV
                     VIKNRNGLHVRPASRLVYTLSTFNADMLLEKNGKCVTPESINQIALLQVRYNDTLRLI
                     AKGPEAEEALIAFRQLAEDNFGETEEVAPPTLRPVPPVSGKAFYYQPVLCTVQAKSIL
                     TVEEEQERLRQAIDFTLLDLMTLTAKAETSGLDDIAAIFSGHHTLLDDPELQAAASEL
                     LQHEHCTAEYAWQHVLKELSQQYQQLDDEYLQARYIDVDDLLHRTLVHLTQTKEELPQ
                     FNSPTILLAENIYPSTVLQLDPAVVKGICLSAGSPLSHSALIARELGIGWICQQGEKL
                     YAIQPEETLTLDVKTQRFNRQG"
     gene            3220..4917
                     /gene="treA"
                     /locus_tag="PN977_004782"
     CDS             3220..4917
                     /gene="treA"
                     /locus_tag="PN977_004782"
                     /EC_number="3.2.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase"
                     /protein_id="HDI6044711.1"
                     /translation="MKSPTPSRPQKMALIPACIFLCFAALSVQAEETSVTPQPPDILL
                     GPLFNDVQNAKLFPDQKTFADAVPNSDPLMILADYRMQQNQSGFDLRHFVNVNFTLPK
                     EGEKYVPPEGQSLREHIDGLWPVLTRATENTEKWDSLLPLPEPYVVPGGRFREVYYWD
                     SYFTMLGLAESGHWDKVADMVANFAHEIDTYGHIPNGNRSYYLSRSQPPFFALMVELL
                     AQHEGDAALKQYLPQMQKEYAYWMDGVENLQAGQQEKRVVKLQDGTLLNRYWDDRDTP
                     RPESWVEDIATAKSNPNRPATEIYRDLRSAAASGWDFSSRWMDNPQQLNTLRTTSIVP
                     VDLNSLMFKMEKILARASKAAGDNAMANQYETLANARQKGIEKYLWNDQQGWYADYDL
                     KSHKVRNQLTAAALFPLYVNAAAKDRASKMATATKTHLLQPGGLNTTSVKSGQQWDAP
                     NGWAPLQWVATEGLQNYGQKEVAMDISWHFLTNVQNTYDREKKLVEKYDVSATGTGGG
                     GGEYPLQDGFGWTNGVTLKMLDLICPKEQPCDNVPATRPLSESTTQPLKQKEAEPTP"
     gene            5247..6269
                     /locus_tag="PN977_004783"
     CDS             5247..6269
                     /locus_tag="PN977_004783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter substrate-binding protein"
                     /protein_id="HDI6044712.1"
                     /translation="MSLTRRRFTQILASTLFLHHLPSFAQSVKFWSSLTLPEAQNITR
                     IVSAGAPADLLLLAVAPEKMVGFSSFDFARQALIPLPEHIRQFPRLGRLAGRASTLSL
                     EGLMALHPDLVVDCGNTDETWISQARQVSEQTQIPWLLLNGKLEQSAEQLTTLGKTLG
                     EEHRAAEQANLASRFVGEAQAFATSPAANLSFYAARGPRGLETGLQGSLHTEAAELLG
                     LHNVAQIADRHGLTQVSMENLLRWQPDIILVQEAVTADFIRRDPLWQGVKAVAEQRIL
                     FLSGLPFGWLDAPPGINRLLGLRRLHAWLDPAINRQFKSDMQHYAQLFWHCSLSDADY
                     QKLVAS"
     gene            6269..>6826
                     /locus_tag="PN977_004784"
     CDS             6269..>6826
                     /locus_tag="PN977_004784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HDI6044713.1"
                     /translation="MRTVNGCILLLAAISITFAAVSGAYHLDMQQLLALILRQENVPV
                     QEQIVFWQIRVPRILAALFLGAALAGAGTTYQGMFRNPLVSPDILGVSAGAGLGACAA
                     ILWGLSIVYIQLYAFCGGLMVVAGVWLITRRVTRHDPILTLVLVGIALGTLCGAGISL
                     IKTLADPYPQLPSITFWLLGGLSTVT"
BASE COUNT         1706 a         1744 c         1712 g         1664 t
ORIGIN      
        1 gacgcccgat aaaatctttg aatgcgccat gcaaattgat ggcggcgaag gtgtactgtt
       61 gattatcaaa aattacaccg gcgatattct taacttcgaa acagcaaccg agttactgca
      121 cgatagcggc gtaaaagtga ccactgtggt cattgatgac gacgttgcag taaaagacag
      181 tctttatacc gccgggcggc gcggcgttgc caacaccgta ttaattgaaa aactcgtagg
      241 tgcagcggcg gagcgtggcg actcactgga cgcctgtgcg gaactggggc gtaagttgaa
      301 taatcaaggc cattcaatag gtatcgctct cggtgcctgt actgttcctg ccgcgggcaa
      361 accttctttt accctggcgg ataatgagat ggaatttggc gtcggcattc atggtgagcc
      421 aggtattgac cgccgcccct tctcttccct tgatcaaacc gtcgatgaaa tgttcgacac
      481 cctgctggaa aatggctcat accatcgcac tttgcgtttc tgggattatc aacaaggcag
      541 ctggcaggaa gaaccacaaa ccaaacaacc gctccagtct ggcgatcggg tgattgcgct
      601 ggttaacaat cttggcgcaa ctccgctttc tgagctgtac ggcgtctata accgcctgac
      661 cacacgttgc cagcaagcgg gattgactat cgaacgtaat ttaattggcg cgtactgcac
      721 ctcactggat atgaccggtt tctcaatcac cttactgaaa gttgatgacg aaacgctggc
      781 actctgggac gccccggtcc acaccccggc ccttaactgg ggtaaataag gagatagcta
      841 tgtcactgag cagaactcaa attgttaact ggctcactcg ttgtggcgat attttcagca
      901 ccgagagcga gtatcttacc ggactggatc gcgaaattgg cgacgctgac cacgggctaa
      961 atatgaaccg aggctttagc aaagtggtgg aaaaactccc tgctatcgca gataaagata
     1021 tcggtttcat tcttaagaat accggtatga cgctactttc cagcgtcgga ggtgccagtg
     1081 gtccgctgtt cggtaccttc tttatccgcg ccgcacaggc gacccaggca cggcaaagcc
     1141 tgacgctgga agagctttat cagatgttcc gcgatggcgc ggacggcgta atcagtcgcg
     1201 gtaaagccga acctggcgat aaaaccatgt gtgatgtctg ggtgtcggtg gtggaatcgt
     1261 tacgtcagtc ctgcgagcaa aatctttctg ttccggcggc cctcgacact gccagtagca
     1321 tcgccgaatc cgctgcacaa agtacgatta cgatgcaagc ccgcaaaggc cgcgccagtt
     1381 atctcggtga acgcagtatt ggtcaccagg atcccggcgc gacctcggtg atgtttatga
     1441 tgcaaatgtt ggcgttagcc gcaaaagagt aaggaattgg tgatggtaaa cctggtcata
     1501 gtttcacata gcagccgact gggagaaggt gtcggtgaac tagcccgtca gatgttaatg
     1561 ggtgatattt gtaaaatcgc cattgccgcg ggaattgacg atccacaaaa tcccattggt
     1621 accgatgccg tcaaagtgat ggaggccatc gaatctgttg ctgatgccga ccatgtgctg
     1681 gtcatgatgg atatgggtag cgcattattg agtgctgaaa ctgcgctgga attgctggct
     1741 cccgagatcg ccgcaaaagt acgtttgtgt gctgcgccgt tggtcgaagg tacactggca
     1801 gcaacggtca gcgcggcctc gggggcggat atcgacaaag tcatctttga cgccatgcat
     1861 gcgctggaag ccaaacgtga acaactgggt ttaccgtcct ccgacactga aatctctgac
     1921 acatgtcctc cgtacgatga agaagcccgt tctctgtcgg tggtcataaa aaaccgtaac
     1981 ggcctgcatg tacgtccggc ctcccggctg gtttatacct tatcgacatt taatgccgat
     2041 atgttgctgg agaaaaacgg caaatgcgtt acaccggaga gtattaacca gattgcgtta
     2101 ctacaagttc gctataacga tacgctgcgc ctgattgcga aaggaccaga agctgaagag
     2161 gcactgatcg ccttccgtca gctggctgaa gataactttg gtgaaacgga ggaagttgct
     2221 ccacctactc tgcgtcccgt tccgcctgtt tcgggtaaag ccttttatta tcaacctgtt
     2281 ttatgtacgg tacaggcaaa atcaatcctg accgtggaag aagaacaaga acgattacgc
     2341 caggccattg acttcacgtt attagatctg atgacgttaa cagcgaaagc agaaaccagc
     2401 gggcttgacg atattgccgc aatcttttct ggtcaccata cactgttaga tgatccggaa
     2461 ctgcaggcgg cggcaagcga actccttcag catgaacatt gcacggcgga atatgcctgg
     2521 caacacgttc ttaaagaact tagccagcaa taccaacagc tggatgatga atatctacag
     2581 gctcgctata tcgatgtgga cgatcttctg catcgcaccc tggtccacct gacccaaacg
     2641 aaagaagaac tcccgcagtt taactcacca actattctac tggcggagaa catttatccc
     2701 tccacagtac tgcaactgga tccagcggtt gtaaaaggta tctgccttag cgccggaagt
     2761 ccgctatccc acagtgccct aatcgcccgt gaactgggaa ttggctggat ttgccagcag
     2821 ggtgagaaac tgtatgcgat acaaccagaa gaaacgctaa cgctggacgt taaaacgcaa
     2881 cgtttcaacc gtcagggtta aaccaccgtt cggatggcat cattctgatg tcatccggca
     2941 atttactctg cactgatgtt tttaactacc tgttatattt ttcaccaaat tatgatgatt
     3001 tttcttcgct aatcgcgaac ccaacgtcgc tggctggcta ataaacagac gttatttaat
     3061 cactctattt cgccgttatt tatcccttta attccctttt ctaaaatgtc tgtcagttcg
     3121 cagaatgtga tttcgatcat gcagctagtg cgatcctgaa ctaaggtttt ctgatacttg
     3181 aataccgttt tattcggttt cgccaaagga gaatgattga tgaaatcccc caccccttct
     3241 cgcccgcaaa aaatggcgtt aattccagcc tgcatctttt tgtgttttgc tgcgctatcg
     3301 gtgcaggcag aagaaacatc ggtaacgcca cagccgcctg atattttatt agggccgctg
     3361 tttaatgatg tgcaaaacgc caaacttttt ccggaccaaa aaacctttgc cgatgccgtg
     3421 ccgaacagcg atccgctgat gatccttgct gattatcgga tgcagcaaaa ccagagcgga
     3481 tttgatctgc gccatttcgt taacgtcaat ttcaccctgc caaaagaggg cgagaaatat
     3541 gttccgccag aggggcagtc actgcgcgaa catattgacg gactttggcc ggtattaacg
     3601 cgtgctaccg aaaataccga aaaatgggat tctctgttac cgctgccgga accttatgtc
     3661 gtgccgggcg gacgttttcg cgaggtatat tactgggaca gttacttcac catgttagga
     3721 cttgccgaaa gcggtcactg ggataaagtc gcggatatgg tggccaattt tgctcatgaa
     3781 atagacactt acggtcatat tcccaacggc aaccgcagtt actatttaag ccgctcgcag
     3841 ccgcccttct ttgccctgat ggtagagtta ctggcgcagc atgaaggcga tgccgcgttg
     3901 aaacaatacc tgccgcaaat gcaaaaagaa tatgcttact ggatggacgg tgttgaaaac
     3961 ctgcaagccg gacaacagga aaaacgcgtt gtcaaacttc aggatggtac ccttctcaac
     4021 cgctactggg acgatcgcga tacgccacga ccagagtcat gggtggaaga tattgccacc
     4081 gccaaaagca atccgaatcg acctgccact gaaatttacc gcgatctgcg ctctgccgct
     4141 gcgtctggct gggatttcag ctcgcgctgg atggacaatc cgcagcagtt aaatacctta
     4201 cgcaccacca gcatcgtacc ggtcgatctg aacagcctga tgtttaaaat ggaaaaaatc
     4261 ctcgcccgcg ccagcaaagc tgccggagat aacgcgatgg caaaccagta cgaaacgctg
     4321 gcgaatgccc gtcaaaaagg gatcgaaaaa tacctgtgga acgatcaaca aggctggtat
     4381 gccgattacg acctgaaaag tcataaagtg cgcaatcagt taaccgcggc cgccctgttc
     4441 ccgctgtacg tcaatgcggc agcgaaagat cgcgccagca aaatggcgac ggcgacgaaa
     4501 acacatctgc tgcaacccgg cggcctgaac accacgtcgg tgaaaagtgg acaacaatgg
     4561 gatgcgccaa acggctgggc accgttgcag tgggtcgcga cagaaggatt acaaaactac
     4621 gggcaaaaag aggtggcgat ggacattagc tggcacttcc tgaccaatgt tcagaacacc
     4681 tatgaccggg agaaaaagct ggtggaaaaa tatgatgtca gcgccaccgg aacagggggc
     4741 ggcggtggcg aatatccatt acaggatggc tttggctgga ccaatggcgt gacgctgaaa
     4801 atgctggatt tgatctgccc gaaagagcaa ccgtgtgaca atgttccggc gacgcgtcca
     4861 ctcagtgaat caacaacaca gccgctaaaa caaaaagagg cggaacctac gccttaactt
     4921 tactgtatat cgagcaatgc cattacatgc ataaaattgg cattgctctt tcttcaccac
     4981 gaaataccac tctttctacg gtctatgcct ttattttctt ttattagaga agattttaat
     5041 tttattttat caacttgtta atcattacac ctctatttat attttccccc actccccatt
     5101 ctccctgaca attttcttgt ggaacaaagt gttatttaag aaacaagcaa atcattctgt
     5161 ctctttagac aaattttgac atcaaacatg atctttttcg ctatatgtgt aagtatatag
     5221 cggttttaat tatcaggata ttacttatgt cactaaccag acggaggttt acacagattc
     5281 ttgcgtcgac attgttcctg catcatctgc cgtcctttgc acaatcagtc aaattctggt
     5341 cctcactgac gcttcccgaa gcccaaaaca ttacacgcat cgtcagcgca ggcgcgcccg
     5401 ccgatttact attgctggct gtcgcgccag aaaaaatggt cggtttttcc tcttttgatt
     5461 ttgcccgtca ggcattaatt ccattgccag agcacattcg ccagttcccc aggctgggac
     5521 gactcgccgg gcgcgccagc acactctcgc tggaagggct tatggcgtta catcccgatt
     5581 tggtcgttga ttgcggcaat acggatgaaa cctggatctc ccaggcacgg caggttagcg
     5641 aacagacaca aataccctgg ttattgctta acgggaaact ggaacaatca gcagaacagt
     5701 taacaacgct tggcaaaacg ttaggcgaag agcaccgcgc cgcagaacaa gccaatctcg
     5761 ccagccgctt cgttggtgaa gctcaggcat tcgccacctc acccgccgct aacctcagct
     5821 tttatgctgc gcgcggtcct cgagggctgg aaacgggctt acagggttcg ttgcataccg
     5881 aggcggcgga attattaggt ttgcacaacg tcgcgcaaat agccgatcgc cacggtctga
     5941 cacaggtttc catggaaaat ctcctgcgct ggcagccgga tattattctg gttcaggagg
     6001 ccgttactgc agattttatt cgtcgtgatc ctctctggca gggcgtgaaa gcggttgcgg
     6061 aacaacgcat cttattttta agtggcctgc cctttggctg gctggatgcc ccgccgggaa
     6121 tcaaccgtct tctgggatta cgcagacttc acgcctggct ggatcccgcc atcaatcgcc
     6181 agtttaaaag tgacatgcag cattacgccc aactgttctg gcattgttca ctcagtgacg
     6241 ccgactatca aaaattggtg gcgagctaat gagaaccgta aacggatgta ttttgctgct
     6301 ggcagctatc agtatcactt ttgccgccgt aagtggggct tatcatctcg atatgcagca
     6361 actgctggcg ctcattcttc gtcaagaaaa tgttcctgtg caggaacaaa ttgtcttctg
     6421 gcaaatccgc gtgccgcgta ttcttgctgc gctgtttctg ggcgcggcgc tggcaggggc
     6481 tggaaccacc tatcagggaa tgttccgtaa tcccttagtg tcaccggata ttttaggcgt
     6541 ctccgccggt gccggacttg gtgcctgcgc ggcaatcctg tggggattat ccattgttta
     6601 catccagctg tacgcatttt gcggcgggct gatggtggtc gcgggcgtct ggttgattac
     6661 gcgtcgggtg acccgccacg atccgatctt aaccctggtg ctggtaggta ttgcgttagg
     6721 cacgttgtgc ggagcgggga tatcgctaat aaaaacgctg gccgatcctt acccccagtt
     6781 accgtcaatt accttctggt tgctgggggg gctgtccacc gtgacc
//
LOCUS       DAMZFT010000206         6724 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.206, whole genome shotgun sequence.
ACCESSION   DAMZFT010000206 DAMZFT010000000
VERSION     DAMZFT010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6724)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6724)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6724
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.206"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            392..2053
                     /locus_tag="PN977_004785"
     CDS             392..2053
                     /locus_tag="PN977_004785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000948448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6044714.1"
                     /translation="MLSGITDTRIDKYSNNEKVTLKSDYSQNECAIYYNSSEMLENTV
                     DLRVSWKDGSNLKLLLPKPVSGGRFVTHDGSVHFDGVASIAHLHGIDAELLTISCAGR
                     GYLNIELLDENPVAEKFRYLHADLPLLSGCNDKLKQISLYENYNLLNAMLACAWSSNS
                     TLCVDFYSDRFGKDKATLSIKRYDGSFIEHDQGLLVDIKNSVVFPANRIDELVVDAIS
                     LKDPGLHISLLKKDEFAYDLSALNVQDSPWLIVGKLDGTARIAPVIKWGLPVLQTNDL
                     LLNALCEADSEQRKKYFNELIFEIDNNPLQNYCCLLTEYIKKYKMNNGLSLLDLDLFR
                     GISSNYRVVVQLLISSCLSGDSDTIYDIQEELPFSWGWIPVSIWKDVFQKCWAYLEKQ
                     INDKTLALHILQPFIAFMNHRAHIDRRLAPIANMLLTYSKLLPAGCNVLPTVSREQFN
                     EAKQMLLRNPDSFGRISIFPKELWSSAITPELKSVFNKLWIKNKYHSRLEKRFNLMLV
                     AALLTQKDNNLIHQLSALFEFHYQQAPQQLGVIYQYYFEQAGVCH"
     gene            2053..3189
                     /locus_tag="PN977_004786"
     CDS             2053..3189
                     /locus_tag="PN977_004786"
                     /inference="COORDINATES: protein motif:HMM:NF012492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HDI6044715.1"
                     /translation="MDVQQLHYAPLLNQLHQRAVRSVVSQLALRSKSLTHYLESQYFQ
                     PPGQEGSLLADPVFESTFGWMPADETMEDLRDKLISSPLVDALSKAPVPFRHQLTAWR
                     TILEDKKSLLVSSGTGSGKTECFMVPVLEDLIRQQKSTRQSLTGTQAIFLYPLNALIN
                     SQQERLREWTRGFKGKIRFALYNGETRHTKYEVQEDQLKVPEQALSREAIYEAPPSIM
                     VTNTTMLEYMLIRRKDAPIIEKSQGMLKYIVLDEAHSYIGSQAAELALLLRRVMQAFN
                     VGPGTDKPVQIIATSATIGEDSPEGNKELAKFVADLAGVTERDVKVVRGYRQIPRVSE
                     SLIRHEYPTSLTSLKSLSPQDLYQQLCHYRVAQQLRQALTHPGR"
     gene            3970..>6724
                     /locus_tag="PN977_004787"
     CDS             3970..>6724
                     /locus_tag="PN977_004787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001417365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HDI6044716.1"
                     /translation="MLTFTDSRQGTARISARLQQDADKATLRALCYQELANINEPSAK
                     LSPAHCALLESFITQFSILPLKNSIDALKKAMVANEELSPADIQALKIIEPMVSSLSP
                     DHAEAMQILQANSGNQQKKTMSWQTLSEQLVGSVDLADRMRGSFKELSGLDLTKQQFA
                     DFCLYSEFGRRPKNAWTLESLGLVAIHYPFIDKVTTCPQDWKTLLPDPDKQLAEWRNL
                     LKITLDFFIRENSAVFYENEQYPRWMGARFPVKMLQGPDKKNKGQKRDQLWPQIRDRH
                     YNRVIKLLIAVFPAIQPEQAHWKSLVNHLMIEVWDAIRPCLRQFESGYQLDIKQQAEF
                     YSPKQVWRCPYTRRALDVTLLGYSPYLPGSKEIAPEKAVLIEMPELPVRHWRLSGGGE
                     IAREERLEWLESNALIQHAREEGLWSTRSDRLALKDSWYRLEEHSAQRTPEQNQFNEK
                     QFKSGKVNVLNCSTTMEMGVDIGGMSLVAMNNVPPAPANYLQRAGRAGRRGESASAAI
                     TLCKNTAHGMEVFKDPLWAFNTTASAPRVRFGSSSIVQRHVNALVLGLFLRAEVPDAT
                     KLSCKWFFEGDESQCLRFLHWLNNQADQLADKLKRLTQGTVLMSLTATQLLTRTQAMM
                     QQVDIRWRSQLAILLENIEALKADNSAWEETPAGKAIAYQLRDYRGAYLFSKLISEAF
                     LPGHGFPVGVVNFNYLTADELEKRRAIKATQADPNEGGESFSRRIEKLPSRDLPTALR
                     EYAPGADVVLGGKVYRSSGIMLGKVLASGQELSGDHHIPWFWHCRKCGAGATSTTRPV
                     ECSHCKADIQQLDVKRYLQPVGFATDIRYQAHNDVSMPAQLPWKDPRVLVPSSVWVSL
                     PDAGLGRYRFSHSGELFHFSEGEFGHGYAICLSCGRAESQNQPQRTPENLKNPERENT
                     HYLL"
BASE COUNT         1856 a         1375 c         1610 g         1883 t
ORIGIN      
        1 taaaataagt ggagtattta gcgttgtttt acatgatggt gcagtttgta caattcgcac
       61 ccagcaactt tatgattctg cgattgaata ttatattaaa tcgacagaag ttgaactggt
      121 taaatcggat tatcctgtcc atcgagcctg gccgaagatc ggttggaaaa aggatctgca
      181 atatggcatt gtaccggaga aagaactttt ttggcgttcc attcgttcag gtaacaatgc
      241 ctggtattct gtcgcatcaa aaatgccaaa aggacagata gaagtcagac gaatagttaa
      301 tgatgaggtt ttatttagcg gtaaagttgt agttttacca gctgacttcg atattaatat
      361 tatacctgag agtgctcagc aaggcatcat aatgctttct ggtataacgg atactagaat
      421 tgacaaatat tcaaacaatg aaaaagtaac acttaagtcc gattattcac aaaacgagtg
      481 tgctatttat tataattcat cggagatgct ggaaaatacc gttgatttac gggtctcctg
      541 gaaagatggt tctaatctta aactattatt acctaagccg gttagcggtg gccgatttgt
      601 aactcatgat ggttctgttc attttgatgg tgtggcatct atagcgcatt tgcatggaat
      661 agatgctgag ttgttaacca tatcttgtgc tggaagagga tatcttaata tcgagttgtt
      721 ggatgaaaat ccagtagctg aaaaattccg ttatttacat gccgaccttc ctcttttatc
      781 aggatgcaat gacaaattaa aacaaatttc actttatgag aactataatt tgctaaatgc
      841 tatgctggca tgtgcatgga gcagcaatag tacattatgt gttgattttt actctgacag
      901 atttggaaag gataaagcaa cactcagtat taaacgctac gatggtagtt ttattgaaca
      961 cgatcaaggg ttactggttg atataaaaaa ttctgttgtt tttcctgcaa atagaataga
     1021 cgagctggtt gttgatgcta tttctcttaa ggatcctggc ttacacatat cgttgctaaa
     1081 aaaagatgaa tttgcttatg atctttcagc tctgaatgtt caggatagcc catggttaat
     1141 tgtggggaaa cttgatggta cagctcgcat tgcaccagta attaaatggg ggctacctgt
     1201 attacagaca aatgatttat tacttaatgc tctatgcgaa gcggattccg aacagcgaaa
     1261 aaaatatttt aatgagctaa tttttgaaat agataacaat cctttgcaaa attattgctg
     1321 tttattaacg gagtatatta agaaatacaa aatgaataat ggcttatcct tgctggatct
     1381 ggatttgttc agaggtattt cgagtaatta ccgcgtagtt gttcagttgt taatatcatc
     1441 atgtctttct ggtgatagcg atacgattta tgatatacag gaagaattac ccttttcatg
     1501 gggatggatt cccgtttcaa tctggaaaga tgtttttcaa aagtgttggg cttatctgga
     1561 aaaacagatt aacgataaaa cattagcatt acatatattg caacccttta ttgcttttat
     1621 gaaccatcgt gcacatatcg atcgtcgtct ggctcccatt gcgaatatgt tacttacata
     1681 tagcaaactc ctaccagccg gttgtaatgt cttgccaact gttagtcgtg agcagtttaa
     1741 tgaagctaaa cagatgctat taaggaaccc cgacagcttt gggcgtatca gtatcttccc
     1801 taaagaactt tggtctagtg cgattactcc agagttaaaa tctgttttta ataagctttg
     1861 gattaaaaat aaatatcact cacggcttga aaaacgtttt aatttgatgt tagtcgcagc
     1921 gctgttaacc caaaaagata ataacttgat acatcaactg tctgcgcttt ttgaattcca
     1981 ctatcagcaa gccccgcagc aattaggggt aatctatcaa tattattttg aacaagcagg
     2041 agtatgtcat tgatggacgt tcagcaactg cattatgcgc cacttctaaa tcagcttcat
     2101 cagcgcgcgg tgaggtcggt ggtaagtcag ttggcattgc ggagcaagtc gttgactcat
     2161 tatctggaat cacaatattt ccagcctccc ggtcaggagg ggtcactgct tgcagacccg
     2221 gtattcgaaa gcacgttcgg ctggatgcct gcggatgaaa caatggagga tttgcgggac
     2281 aagctaattt ccagcccgct ggttgatgca ttgagcaaag cgccggtgcc atttcgtcat
     2341 caacttacgg cgtggcgaac gattctggaa gacaaaaaat ccttgttagt gagcagcggt
     2401 acgggatccg ggaaaacaga atgctttatg gtaccggttc tggaagatct tattcgtcag
     2461 caaaaatcga cccgtcagtc actaacaggg acacaagcta ttttcttgta tccgttaaat
     2521 gcattaatta acagccaaca ggagcggctt cgcgaatgga cgcgcggatt taaggggaaa
     2581 atacgcttcg cgctatataa tggcgaaacc cggcatacca aatacgaagt tcaggaagat
     2641 cagttgaaag tacctgaaca agctttgagt cgggaagcca tttacgaagc accaccttcc
     2701 atcatggtga cgaataccac catgctggaa tatatgctga ttcgtcgtaa agatgcccca
     2761 atcatcgaaa agtctcaggg gatgttaaag tatatcgtcc tggatgaagc ccatagttat
     2821 attggttctc aggctgctga gttagcgctg cttttacgtc gtgttatgca ggctttcaat
     2881 gttggaccgg gcacagataa acctgtgcag attatcgcga cctctgccac tattggtgaa
     2941 gactctcctg aaggtaataa ggagcttgct aaatttgtcg ctgatctcgc gggcgttaca
     3001 gaacgtgatg tcaaagtggt tcgcggctat cgacaaatac cgcgggtatc ggagtcgctg
     3061 attcgtcatg aatatcccac atctttaact tctcttaaat ctttgagccc tcaggatctt
     3121 taccaacagc tttgtcatta ccgtgtggcc cagcaattgc gtcaggcgtt aactcatccc
     3181 ggacggtagg cagtacgttt gagtgagttg ctcaatgttg ccagaagaac ctggccagat
     3241 attaatcacc gtgagttact gcaactcctc gacctaatgg cgagatcgcg agaaggtgaa
     3301 ctggctttta cgccattacg tatgcatggg tttatcagaa cactggcggg tctatgggca
     3361 tgttcaaaca aacaatgctc acataaagcg catgaattaa atcagagtga ctggccattt
     3421 ggtcaggtat ggtttgagca gcgccaatat tgtgactgtg gtgcgccggt atttgaagtt
     3481 ttacgttgct ccgggtgtgg ttcagcttat ttgtctgcca aagaggaggt gcgcggtgat
     3541 ggaacgaact ggttggtagc gcaacccgcc gctgccgaag tggatgaatt tgctcttgat
     3601 gtagatgttt attctgaaga tgaagacaac gacgaattag acaataactc tcaatttgat
     3661 cgtctgattg cgatcgatgg tgaaaaatat atcatttctc tggaagaaac gacggcgggg
     3721 aaaattgata gtgaagcgca aaagcactat gaaataaacc tgttaagacc ggaatctcga
     3781 ggtgagggac gtaataattc ctttgcttgt gtttgctgtg gtgacaccca acgaaaaaat
     3841 aatcctttat tccgtccgct tcgacttggc gcgccctttt ttttaaatga aattattcct
     3901 actctgctgg agttttcacc attaccgcag acaagagaag agcagttagg accgtttaat
     3961 ggtcgacgtc tgctcacctt tacggattca cgtcagggca ctgcccgcat ttctgcccgc
     4021 ttacagcagg atgccgataa agcgacgttg cgagctcttt gttaccagga gttggccaat
     4081 attaatgagc catcagcaaa gctcagcccg gcacactgcg cattgttaga gagttttatt
     4141 acccagtttt ctatccttcc tttaaaaaac tctattgacg cgctgaaaaa agcgatggtt
     4201 gctaatgaag agcttagccc ggcggacatt caggcactta aaattatcga gccgatggtg
     4261 agttccttaa gcccggatca tgctgaagcg atgcaaatac tccaggctaa ctctggcaat
     4321 cagcagaaga aaaccatgtc atggcaaaca ctgtctgaac agctggttgg cagtgtggac
     4381 cttgccgata gaatgagagg cagttttaaa gaattgtctg ggcttgatct tactaaacaa
     4441 cagtttgcag atttttgttt atacagtgag tttggtcgcc gccctaaaaa tgcctggacc
     4501 ctcgaaagtc tgggtctggt agccattcac tatccattta ttgataaagt tacaacctgc
     4561 ccgcaggact ggaagacatt attacccgat cccgataagc aattagcgga atggcgtaat
     4621 ttattgaaga ttactcttga tttctttata cgtgaaaaca gtgccgtttt ttacgaaaac
     4681 gagcaatacc ctcgctggat gggggctcgt ttcccggtaa aaatgctcca gggtccagat
     4741 aaaaaaaaca aagggcagaa acgtgatcaa ctttggccac aaatacgcga tcgccattat
     4801 aaccgggtca ttaagctatt aattgcagtc tttccggcaa tacaacctga gcaggctcac
     4861 tggaaatcgt tagttaacca cttaatgatt gaggtctggg atgcaattcg accctgctta
     4921 cgtcagtttg agtcgggtta tcagctggat attaaacaac aagctgaatt ttacagtcca
     4981 aagcaagtgt ggcgttgccc ctatactcga cgtgcgttag acgtcacgtt actgggatac
     5041 tctccgtatt tgccgggtag caaagaaatt gcacctgaaa aagcagtgtt gattgaaatg
     5101 cctgagcttc ctgtacgtca ctggcgtttg tctggtggtg gagaaatcgc ccgagaagaa
     5161 cgtctggagt ggctggaaag taatgcgctg attcaacatg cccgcgagga aggattatgg
     5221 tctactcgca gcgaccgact ggccctgaaa gatagctggt atcgtctgga ggaacactct
     5281 gcgcagagaa cgcctgagca gaaccagttt aatgaaaaac aatttaagtc aggcaaagtt
     5341 aatgtgctga actgctccac gaccatggag atgggggtgg acattggtgg tatgagtttg
     5401 gtggcaatga acaacgttcc tccagctccg gcaaattatt tgcagcgtgc aggccgcgcg
     5461 ggtcgacgtg gagagagtgc atctgctgcc attacgttat gtaaaaacac ggcgcatgga
     5521 atggaggtat tcaaagaccc cctctgggcg ttcaacacta ctgccagtgc gccacgggtt
     5581 cgttttggga gcagcagtat tgttcaacga catgttaatg ccctggtttt aggtctgttt
     5641 ttacgagctg aagttcctga tgcgacaaaa ctgagttgca aatggttctt tgaaggtgat
     5701 gagagccagt gtcttcgttt tctccattgg ttaaataacc aggccgatca gctggcggat
     5761 aaacttaaac gccttactca gggaaccgtc ctgatgtcat tgacggcaac ccagctcttg
     5821 acccgcacac aagcaatgat gcagcaggtt gatatccgct ggagaagcca acttgcgata
     5881 ctgttggaaa atatcgaagc gttgaaggcg gataacagcg cgtgggaaga aacgccagca
     5941 ggtaaagcca tcgcttatca gcttcgtgat tatcgcggtg catatctgtt ttctaaactc
     6001 attagtgaag cgttcctgcc ggggcatggt ttcccggttg gcgtcgtcaa tttcaattat
     6061 ctgacggcag atgagctgga aaaacgtcgc gcaataaaag ccactcaggc tgaccctaat
     6121 gaagggggag aaagcttttc tcgtcgtatt gaaaaattgc ccagccgtga tttgcctaca
     6181 gcattacgcg agtatgctcc aggtgcagat gtggtattgg gcggaaaagt atatcgttcc
     6241 tcgggcatta tgcttggcaa ggtacttgcc agcgggcagg agctgtcagg cgatcatcat
     6301 attccgtggt tctggcattg tcgtaaatgt ggtgcggggg caaccagtac tacccgtcct
     6361 gtcgaatgta gtcactgcaa agcggatata cagcaacttg atgtgaagcg ttatttacag
     6421 cctgttggtt ttgcgacaga tatccgttat caggcgcata atgatgtatc catgcctgca
     6481 caacttccct ggaaagatcc acgtgttctt gtaccgtctt cggtgtgggt ttctttaccc
     6541 gatgctgggc taggacgtta ccgttttagc catagtgggg aattgttcca cttcagtgaa
     6601 ggggaatttg ggcacggcta cgcaatttgc ctctcatgtg gtcgggcaga atctcaaaat
     6661 caaccccagc gtacccctga aaatcttaag aacccagaga gggaaaatac gcattacctg
     6721 ttgc
//
LOCUS       DAMZFT010000207         6716 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.207, whole genome shotgun sequence.
ACCESSION   DAMZFT010000207 DAMZFT010000000
VERSION     DAMZFT010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6716)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6716)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6716
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.207"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            55..753
                     /gene="thiQ"
                     /locus_tag="PN977_004788"
     CDS             55..753
                     /gene="thiQ"
                     /locus_tag="PN977_004788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter ATP-binding protein
                     ThiQ"
                     /protein_id="HDI6044717.1"
                     /translation="MLKLTDITWLYHHLPMRFSLTVERGEQVAILGPSGAGKSTLLNL
                     IAGFLTPASGSLTIDGVDHTTTPPSRRPVSMLFQENNLFSHLTVAQNIGLGLNPGLKL
                     NSAQQEKMHAIARQMGIDNLMARLPGELSGGQRQRVALARCLVREQPILLLDEPFSAL
                     DPALRQEMLTLVSTSCQQQKMTLLMVSHSVEDAARIATRSVVVADGRIAWQGKTDELL
                     SGKASASALLGITG"
     gene            complement(823..1587)
                     /locus_tag="PN977_004789"
     CDS             complement(823..1587)
                     /locus_tag="PN977_004789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HDI6044718.1"
                     /translation="MQALLEHFITQSTVYSLMAVVLVAFLESLALVGLILPGTVLMAG
                     LGALIGSGELSFWHAWLAGIVGCLLGDWISFWLGWRFKKPLHRWSFLKKNKALLDKTE
                     HALHQHSMFTILVGRFVGPTRPLVPMVAGMLDLPVAKFITPNIIGCLLWPPFYFLPGI
                     LAGAAIDIPAGMQSGEFKWLLLATAVFLWVGGWLCWRLWRSGKATDRLSHYLSRGRLL
                     WLTPLISAIGVVALVVLIRHPLMPVYIDILRKVVGV"
     gene            complement(1742..2164)
                     /locus_tag="PN977_004790"
     CDS             complement(1742..2164)
                     /locus_tag="PN977_004790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000049682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6044719.1"
                     /translation="MSKYIYILLSFLVLFFIFFYAYISLMSKEHHYTQHELSPFFLYT
                     PESLRNLPNISNVAEYSYYYNVDDMQTRVIVTWRNIDNIFLQKAKLIDFLKGMGPSLQ
                     NDCIWFFHDKSDYANNFQRYCIIEHRDSLQVEYFETIE"
     gene            complement(2171..3019)
                     /locus_tag="PN977_004791"
     CDS             complement(2171..3019)
                     /locus_tag="PN977_004791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000044502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6044720.1"
                     /translation="MSKRNDITDGIFATTKKYGLVYTEELGWIDLGHAQGQDARILKR
                     KLEQEHFSTYYDEFHDWYFPVDYHQEMGIREKILGVDLTFHTGVYTKVMVRSCLSPTL
                     KARVALTLMYGTAKRFEAWQNSFIFNWYTDSGFSAEDLVSDLIGFYRVFGTGPDPLLL
                     AKPLSYTKALQIWDTYGAPGNFKNTEFTPFLFTTHPPFKKNQLIKKKLPEWLNYIKPL
                     DESFSTLLYNQYNNRPITNYYKDKNRINHELYSSLSSSGAIKFSESPFERPLFLFLNP
                     HYPHRS"
     gene            complement(3533..4378)
                     /gene="araC"
                     /locus_tag="PN977_004792"
     CDS             complement(3533..4378)
                     /gene="araC"
                     /locus_tag="PN977_004792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose operon transcriptional regulator AraC"
                     /protein_id="HDI6044721.1"
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKG
                     YILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRA
                     YWHEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLL
                     LRRMEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQ
                     LGISVLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEF
                     RAGCE"
     gene            4717..6417
                     /gene="araB"
                     /locus_tag="PN977_004793"
     CDS             4717..6417
                     /gene="araB"
                     /locus_tag="PN977_004793"
                     /EC_number="2.7.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulokinase"
                     /protein_id="HDI6044722.1"
                     /translation="MAIAIGLDFGSDSVRALAVDCATGEEIATSVEWYPRWQKGQFCD
                     APNNQFRHHPRDYIESMEAALKTVLAELSAEQRAAVVGIGVDTTGSTPAPIDADGNVL
                     ALRPEFAENPNAMFVLWKDHTAVEEAEEITRLCHTPGNVDYSRYIGGIYSSEWFWAKI
                     LHVTRQDSAVAQSAASWIELCDWVPALLSGTTRPQDIRRGRCSAGHKSLWHESWGGLP
                     PASFFDELDPILNRHLPSPLFTDTWTADIPVGTLCPEWAQRLGLPESVVISGGAFDCH
                     MGAVGAGAQPNALVKVIGTSTCDILIADKQSVGERAVKGICGQVDGSVVPGFIGLEAG
                     QSAFGDIYAWFGRVLGWPLEQLAAQHPELKEQINASQKQLLPALTEAWAKNPSLDHLP
                     VVLDWFNGRRTPNANQRLKGVITDLNLATDAPLLFGGLIAATAFGARAIMECFTDQGI
                     AVNNVMALGGIARKNQVIMQACCDVLNRPLQIVASDQCCALGAAIFAAVAAKVHADIP
                     SAQQKMASAVEKTLQPRSEQAQRFEQLYRRYQQWAMSAEQHYLPTSAPAQAAQAVPTL
                     "
     gene            6428..>6716
                     /locus_tag="PN977_004794"
     CDS             6428..>6716
                     /locus_tag="PN977_004794"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414604.1"
                     /note="catalyzes the formation of L-ribulose from
                     L-arabinose in L-arabinose catabolism; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HDI6044723.1"
                     /translation="MTIFDNYEVWFVIGSQHLYGPETLRQVTQHAEHVVNALNTEAKL
                     PCKLVLKPLGTTPDEITAICRDANYDDRCAGLVVWLHTFSPAKMWINGLTML"
BASE COUNT         1785 a         1729 c         1571 g         1631 t
ORIGIN      
        1 tgctgctact ctgttttctg ctgtttactg tgattgaaaa actaccgggg cgaaatgtta
       61 aaactgactg atatcacctg gctttatcac catttgccga tgcgttttag cttaacggta
      121 gaacgcggcg agcaggtggc gatcctcggg ccaagcggcg cgggtaaaag taccctgctg
      181 aatttgatcg ccggttttct gacgccagcc agcggttcgc tgactatcga tggcgttgat
      241 cacaccacga cgccgccgtc acgccgtccg gtgtcgatgc tgtttcagga gaacaacctg
      301 ttcagtcacc tgacggtcgc gcagaacatt gggctggggc taaatccggg attgaaacta
      361 aattcggcgc agcaggagaa aatgcacgct atcgcccgtc aaatgggaat tgataattta
      421 atggcgcggt taccgggcga gctttccggc ggtcagcgac agcgcgtggc gttagcgcgt
      481 tgtctggtac gcgaacagcc gattttattg ctcgatgaac cgttctctgc gctcgatccg
      541 gcgttacgcc aggagatgtt gacgctagtg agcacgagct gccaacagca aaaaatgacg
      601 ctattgatgg tgtcgcacag tgtggaagat gcggcgcgga tcgccacgcg ctcggtggtg
      661 gtcgccgacg ggcgcatcgc ctggcagggt aagactgatg agttgttgag cggtaaggcg
      721 agtgcttcgg cactattggg gattacgggt taaaagcgca cggacagccc cctcgcccct
      781 ccggggtgag gggaacaaac cagcgccggt gcaaactatc tcctaaaccc caaccacttt
      841 acgcaaaata tcgatataca ccggcatcaa cgggtggcga attaacacca ccagcgccac
      901 cacgccgatg gcagaaatca acggcgtcag ccacaacaaa cgaccgcgcg acaaataatg
      961 actcaaacgg tcagtcgctt taccgctgcg ccataaccgc cagcacagcc agcccccaac
     1021 ccacaaaaat accgccgtcg ccagcagcaa ccatttaaac tcaccactct gcatcccggc
     1081 aggaatatcg atcgccgcac ccgccagaat ccccggcagg aagtaaaacg gtggccacag
     1141 caggcagccg ataatattcg gcgtaataaa ttttgccacc gggagatcca gcattcccgc
     1201 caccattggc accagcggac gcgtcgggcc aacaaagcga ccgaccagaa tggtgaacat
     1261 gctgtgttga tgcaacgcat gttcagtttt atcgagtaat gctttgtttt tcttcagaaa
     1321 tgaccagcga tgcaatggct ttttaaaacg ccagcccagc cagaaagaga tccagtcgcc
     1381 cagcaggcaa ccgacgatcc ctgccagcca ggcgtgccag aaacttaact cgccactgcc
     1441 aatcagcgcc cccagtcccg ccattagcac cgtaccgggt aaaatcaaac cgaccagcgc
     1501 cagcgactcc agaaaggcca ccaacaccac cgccatcaat gaatacacgg tggattgggt
     1561 aataaagtgt tccagcaatg cttgcataat gtgcctgtca aatggacgaa gcagggattc
     1621 tgcaaaccct atgctactcc gtcaagccgt caattgtctg attcgttacc aattatgaca
     1681 acttgacggc tacatcattc actttttctt cacaaccggc acaaaagaca ccctctgcct
     1741 atcattcgat agtctcaaaa tattccacct gcagagaatc acggtgctca atgatgcaat
     1801 aacgctggaa attatttgca taatcacttt tatcatgaaa aaaccatata caatcgtttt
     1861 gtagactcgg tcccattcct ttcaaaaaat ctattagctt tgctttctga agaaaaatat
     1921 tatctatatt tcgccatgta acaatcactc ttgtttgcat atcatcaaca ttatagtaat
     1981 agctatactc ggctacgttt gatatatttg gcaaatttcg caaactctct ggcgtataaa
     2041 ggaaaaatgg ggaaagttca tgctgtgtat agtgatgttc ctttgacatc aatgagatat
     2101 aagcatagaa aaatataaag aataaaacta aaaatgataa taaaatataa atatattttg
     2161 acatctctac tcacgacctg tgcggataat gagggtttaa gaaaagaaaa agcggtcttt
     2221 caaagggaga ttctgaaaac tttatagctc ccgaacttga taatgaacta tacaactcat
     2281 gatttattct gtttttatct ttataataat tagtaatagg tctattatta tattgattat
     2341 ataacaatgt tgaaaaagac tcatctaatg gctttatata atttaaccat tctggaagct
     2401 tcttttttat tagttgattt tttttaaaag gaggatgggt tgtaaataag aatggcgtaa
     2461 actcagtatt tttaaaattc cctggcgctc catatgtatc ccatatttgt aaggcttttg
     2521 tgtaagataa aggttttgcc aataaaagtg gatcggggcc tgttccaaat acacgataaa
     2581 aaccaatcaa gtctgagact aaatcttctg cactaaatcc gctatcagta taccagttaa
     2641 aaataaatga gttttgccat gcttcaaacc tcttcgcagt tccatacatt aacgttaagg
     2701 caactcttgc tttaagagtg ggtgaaagac atgaacgaac cataaccttc gtataaacac
     2761 cggtatgaaa tgtaagatct actcctaata ttttttcccg aattcccatt tcctgatgat
     2821 aatcaacagg aaaataccaa tcatgaaatt catcgtaata tgttgaaaaa tgttcttgct
     2881 ccaattttct ttttaatatt ctggcatctt gtccctgagc atgccccaaa tcgatccaac
     2941 ctaactcttc ggtataaact aatccatact ttttcgttgt tgcaaatatt ccatcagtaa
     3001 tatcgttacg tttgctcatc acaatctccc ctcaaaaaaa acgtatagca aataatatca
     3061 ataaggaaaa atattatttt tcaatgtttt tttgtataca attcatttta tgaatgaatt
     3121 tacaaagata gctgcgcctg ataccgctac acttttgcca gaccatgttc tcctctgagg
     3181 gagtaactgg cgcttttcat agaatgccgt acttttttca acaactgcca catcgaacgg
     3241 catttatggt ggcgtgaagt gcctgccata accgctcaat gtggtgttcc tacgatgaac
     3301 aaaccgcttg atatattctc ctgatgcaac cggacaatat gttcatgatg ggtatcccga
     3361 tacataagcc ttcgttttat ttttacaaaa agaaatcgtt gtcgatattt tatcaagaaa
     3421 aaaataattc aggtaaaacg attcaactaa aaataactaa aattaatccc ccgtgaaata
     3481 tataaataac gtcagatgaa gaaagcaagg ggggcccccc cttgctttct tattattcac
     3541 atccagccct aaactcgctc gggctggccc cggtgcattt tttaaatact cgcgagaaat
     3601 agagttgatc gtcaaaacca acattgcgac cgacggtggc gataggcatc cgggtggtgc
     3661 tcaaaagcag cttcgcctga ctaatgcgtt ggtcctcgcg ccagcttaag acgctaatcc
     3721 ctaactgctg gcggaaaaga tgtgacagac gcgacggcga caagcaaaca tgctgtgcga
     3781 cgctggcgat atcaaaattg ctgtctgcga gatgatcgct aatgtactga caagcctcgc
     3841 gtacccgatt atccatcggt gggtggagcg actcgttaat cgcttccatg cgccgcagta
     3901 acaattgctc aagcagattt atcgccagca attccgaata gcgcccttcc ccttgcccgg
     3961 cgttaatgat ttgcccaaac aggtcgctga aatgcggctg gtgcgcttca tccgggcgaa
     4021 agaaacccgt attggcaaat attgacggcc agttaagcca ttcatgccag taggcgcgcg
     4081 gacgaaagta aacccactgg tgataccatt cgcgagcctc cggatgacga ccgtagtgat
     4141 gaatctctcc tggcgggaac agcaaaatat cacccggtcg gcagacaaat tctcgtccct
     4201 gatttttcac caccccctgg ccgcgtatgg tgagattgag aatataaccc ttcattccca
     4261 gtggtcggtc gataaaaaaa tcgagataac cgttggcctc aatcggcgtt aaacccgcca
     4321 ccagatgggc gttaaacgag tatcccggca gcaggggatc attttgcgct tcagccatac
     4381 ttttcatact cccgccattc agagaagaaa ccaattgtcc atattgcatc agacattgcc
     4441 gtcaccgcgt cttttactgg ctcttctcgc taatccaacc ggtaaccccg cttattaaaa
     4501 gcattctgta acaaagcggg accaaagcca tgacaaaaac gcgtaacaaa agtgtctata
     4561 atcacggcag aaaagtccac attgattatt tgcacggcgt cacactttgc tatgccatag
     4621 catttttatc cataagatta gcggatccta cctgacgctt tttttcgcaa ctctctactg
     4681 tttctccata cccgtttttt tggatggagt gaaacgatgg cgattgcaat tggcctcgat
     4741 tttggcagtg attctgtgcg agctttggcg gtggactgcg ccaccggtga agagatcgcc
     4801 accagcgtag agtggtatcc ccgttggcag aaagggcaat tttgtgatgc cccaaataac
     4861 cagttccgtc atcatccgcg tgactacatt gagtcaatgg aagcggcgct gaaaaccgtg
     4921 cttgcagagc ttagcgccga gcagcgcgca gctgtggtcg ggattggcgt tgacacaacc
     4981 ggctcgacgc ccgcaccgat tgacgcggac ggtaacgtcc tggcgctgcg cccggagttt
     5041 gccgaaaacc cgaacgcgat gttcgtattg tggaaagacc acaccgcggt tgaagaagcg
     5101 gaagagataa cccgcttatg tcacacgccg ggcaacgttg actactcccg ctatattggc
     5161 ggtatttatt ccagcgaatg gttctgggca aaaatactgc atgtgactcg acaggacagc
     5221 gccgtggcgc aatctgccgc atcgtggatt gagctgtgcg actgggtacc agctctgctt
     5281 tccggtacca cgcgcccgca ggatattcgt cgcggacgtt gcagcgccgg gcataagtcg
     5341 ctatggcatg aaagctgggg cggcctgccg ccagccagtt tctttgacga actggacccg
     5401 atcctcaatc gccatttgcc ttccccgctg ttcactgata cctggactgc cgatattccg
     5461 gtgggcacgt tatgtccgga atgggcacag cgtctcggcc tgcctgaaag cgtggtgatt
     5521 tccggcggcg cgtttgactg ccatatgggc gcagttggtg caggcgcaca gcctaacgca
     5581 ctggtaaaag ttatcggtac ttccacctgc gacattctga ttgccgacaa acagagcgtt
     5641 ggcgagcggg cagtgaaagg tatttgcggt caggttgatg gcagcgtggt gcctggattt
     5701 atcggtctgg aagcaggcca atcggcgttt ggcgatatct acgcctggtt cggtcgcgta
     5761 ctcggctggc cgctggaaca gcttgccgcc cagcatccgg aactgaaaga gcaaatcaac
     5821 gccagccaga aacaactgct tccggcgctg accgaagcat gggccaaaaa tccgtctctg
     5881 gatcacctac cggtggtgct cgactggttt aacggccgcc gcacaccgaa cgctaaccaa
     5941 cgcctgaaag gggtgattac cgatctgaac ctcgccaccg acgcaccgct gctgttcggc
     6001 ggtttaattg ccgccaccgc ctttggcgca cgcgcaatta tggagtgctt caccgatcag
     6061 gggatcgccg tcaataacgt gatggcactg ggcggcatcg cgcgcaaaaa ccaggtcatt
     6121 atgcaggcct gctgcgacgt gctgaatcgc ccgctgcaaa ttgttgcctc tgaccagtgc
     6181 tgtgcgctcg gtgcggcgat tttcgctgcc gtcgccgcga aagtgcacgc agatatccca
     6241 tcagcccagc aaaaaatggc cagtgcggta gagaaaaccc tgcaaccgcg cagcgaacaa
     6301 gcacaacgct ttgaacagct ttatcgccgc tatcagcaat gggcgatgag cgccgaacaa
     6361 cactatcttc caacttccgc cccggcacag gctgcccagg ccgttccgac tctataagga
     6421 cacgacaatg acgatttttg ataattatga agtgtggttt gtaattggca gccagcatct
     6481 ttacggcccg gagactctgc gccaggtgac gcaacatgcg gaacacgttg ttaatgcact
     6541 gaatacagaa gcgaagttgc cctgcaaact ggtgctgaaa ccgctgggca ccacgccgga
     6601 tgaaatcacc gctatttgcc gcgacgcgaa ttacgacgat cgttgcgctg gtctggtggt
     6661 gtggctgcac accttctccc cggccaaaat gtggatcaac ggcctgacca tgctca
//
LOCUS       DAMZFT010000208         6546 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.208, whole genome shotgun sequence.
ACCESSION   DAMZFT010000208 DAMZFT010000000
VERSION     DAMZFT010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6546)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6546)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6546
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.208"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(<1..609)
                     /locus_tag="PN977_004795"
     CDS             complement(<1..609)
                     /locus_tag="PN977_004795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001075464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus Gp2 family protein"
                     /protein_id="HDI6044724.1"
                     /translation="MNQPIHNAYWLSRFESILNSALAQHRAVSLIRVDLRFPEYMPAT
                     IMDTDLDSAVISRFFASLKAKIQAYQRHKRRANKRVRATTLRYFWCREFGKEKGRKHY
                     HVILLLNKDTWCSPGDFTVPSSLATLIQLAWCSALHLEPWQGNGLVHFSRRTPFRKPT
                     SSDARPSSDDTPLSGGCSETRKASDKKPGESAVLWVKRGDVEA"
     gene            complement(2073..3296)
                     /locus_tag="PN977_004796"
     CDS             complement(2073..3296)
                     /locus_tag="PN977_004796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001702852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6044725.1"
                     /translation="MAKNKGICRNKKCSNPVSEFDEANALRLCDDHYKLWKRNKENRR
                     VRNICRIISRYDKSLDWEIFYRVRDAAKNKTGKRIIEDAKILHQWRASKQVRKSFYKG
                     VLRSREWLNTQELLSRKHISVTGELFKFYIKDIIRLYCYKFLYSREYLNENAYKKRYQ
                     RDIHPRKVNVPILQLEVAWINNNCRSSDSSTDIIILPRMIRRMVVFNYTKINKQLEKV
                     LGGIARKQTSERNKGGLYTRIKKESDFLSMDRFMVEIENIIRPERFPTSIYPVFSHSF
                     IERELPLLNLARSQYKRAISKRVANSIFYTLQKTNVALWLYLEMFALVAFIKLYTGKG
                     FEFIKNMCVFIDYEKGIIKSTHEELLGEIKRNIQVNLKVNPDNFPELIDIYNSCFSEN
                     ILSYDIQDGNFKWRS"
     gene            3397..4281
                     /locus_tag="PN977_004797"
     CDS             3397..4281
                     /locus_tag="PN977_004797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001557377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosome-binding GTPase"
                     /protein_id="HDI6044726.1"
                     /translation="MPFSNSSLSAQVKSYLTFLPEEIRQKILEHLHGVIHYEPVIGIM
                     GKSGTGKSSLCNAIFQSRICATHPLNGCTRQAHRLTLQLGERRMTLVDMPGIGETPQH
                     DQEYRELYRQLLPELDLIIWILRADERAYAADIAMHQFLLNEGADPSRFLFVLSHADR
                     VFPAEEWNDTEKCPSRQQELSLATVTARVATLFPSSFPVLSVAAPVGWNLPALVSLMI
                     HALPPQATSAVYSHIRGENRSEQARKHAQQTFGDAIGKSFDAAVAQFSFPAWMLQLLR
                     KARDRIIHLLVTLWDRLF"
     gene            4484..5164
                     /locus_tag="PN977_004798"
     CDS             4484..5164
                     /locus_tag="PN977_004798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001278649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HDI6044727.1"
                     /translation="MSDNRSRHDRLAVRLSLIISRLMAGESLSLKTLSDEFGVTERTL
                     QRDFHQRLVHLDLEYRNGRYSLRRQSSPGAIPEMLSFIQNTGIARILPLRNGRLITCL
                     TDNQEPSPCLIWLPAPDITATFPECFSQLILAIRQCIHISLMTERWYPSLEPCRLIYY
                     SGSWYLIALQKGKLQVFPLADIKSVSLTSERFERRGHIHSLVAEERFISALPHFSFIH
                     KLINTFNL"
     gene            5312..5989
                     /locus_tag="PN977_004799"
     CDS             5312..5989
                     /locus_tag="PN977_004799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001097314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6044728.1"
                     /translation="MNTRALFPLLFTVASFSASAGNWAVKNGWCQTMTEDGQALVMLK
                     NGTIGITGLMQGCPNGVQTLLGSRISINGNLIPTSQMCNQQTGFRAVEVEIGQAPEMV
                     KKAVHSIAERDVSVLQAFGVRMEFTRGDMLKVCPKFVTSLAGFSPKQTTTINKDSVLQ
                     AARQAYAREYDEETTETADFGSYEVKGNKVEFEVFNPEDRAYDKVTVTVGADGNATSA
                     SVEFIGK"
     gene            5995..6228
                     /locus_tag="PN977_004800"
     CDS             5995..6228
                     /locus_tag="PN977_004800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001278287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 family protein"
                     /protein_id="HDI6044729.1"
                     /translation="MSDCHPVLLPEGPFSREQAVAVTTAYRNVLIEDDQGTHFRLVIR
                     NAEGQLRWRCWNFEPDAGKQLNSYLASEGILRQ"
     gene            6318..>6546
                     /locus_tag="PN977_004801"
     CDS             6318..>6546
                     /locus_tag="PN977_004801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF932 domain-containing protein"
                     /protein_id="HDI6044730.1"
                     /translation="MQLASRFGHVNQIRRERPLTREELMYHVPSIFGEDRHTSRSERY
                     AYIPTITVLENLQREGFQPFFACQTRVRDQSR"
BASE COUNT         1855 a         1573 c         1302 g         1816 t
ORIGIN      
        1 cgcttccaca tcaccacgct tgacccagag cacagcggat tcacccggct ttttgtctga
       61 agccttccta gtttcagaac atccacccga caaaggcgta tcatcggaag aagggcgagc
      121 atcagatgat gtcggtttac ggaaaggcgt ccgcctggaa aaatgaacca gtccattacc
      181 ctgccagggc tcaagatgca gagcactaca ccatgccagt tggatcagcg tcgccagcga
      241 agaaggaacg gtgaaatccc ctggcgagca ccaggtatct ttgttgagca gtaatatcac
      301 gtgataatgt ttcctgcctt tttccttgcc aaactcccga caccagaaat aacgcagagt
      361 ggttgcacgc acacgcttgt tggcacgtcg tttatgtcgc tggtaagcct gaatttttgc
      421 tttcagggat gcaaaaaaac gagaaatcac cgctgaatct aaatcggtat ccatgatggt
      481 ggcaggcata tactcaggga aacgtaaatc cacccggatt aacgagacgg cacggtgctg
      541 cgccagggca ctgttgagaa tactttcaaa acgggataac cagtaagcat tgtgtattgg
      601 ttgattcatg atgagagatc tccgttgatt tggattgact tcacatcatt aatcactgcg
      661 gtaaaacttt acggcatccc gccattgagc aacgccaatg gctcctgatt aaaacagaat
      721 gaatccccca cgaagaaaaa ataaacctac acgcagagta aaacacacaa caacggcata
      781 aatgtttttt aaaaactaca aagcctgaag ggaaacggat caacagaacg cgcaaacaaa
      841 acgtcagaaa cgcaatcaga acgctttctg gcgctgtaaa tcaacagcaa caccacacct
      901 acagcttaac cacacaactc aatcacagag ccgaacagcg cgattaaaac ctgtttacag
      961 acacccccgc tccaagaaca aaaaccacca acatcagaat cgattttctt atactgaaaa
     1021 cgttactttt aactacgctc cataaaaacg gttaaccatc gataacgtaa acctcatctg
     1081 aatcaaaaca ccagaaccaa tcatcacgct tttcctgaaa agagtcttgc gctgagaaaa
     1141 aacaccaggg ccagttaatc atctcatcaa tctcatcgtt acaaaatcat ccataaaacc
     1201 atttaacaaa atcatcgtaa gcacacagta aatcacgcca ggttatctca gccggatttg
     1261 cgtttttaca taaagacact tgtctcaggt taacgactaa aaacacaaaa actaagaaaa
     1321 ggttaaacgt aacttcagaa aaatagaata tcatcctgat tacttaaaca ggaaagattg
     1381 ttttgtagca gctttaaact gattttagct aacgtaaaga tttactaaac tggatgtgta
     1441 tcagatcaaa caatgtgtac agcaatgctt aatgaaaaca cttgtagaat tgtttatgaa
     1501 aatgcgtgta taacacttag tgaagatatg tctgtataat acgtatataa tacgtatata
     1561 gtaccattcc cgacattctc aatatcttca gacatccatg cagactgagt gttgatttca
     1621 gtttttcacg gccagcatgg atctttactg gtcaaaaaac agacaggttt actctggaat
     1681 tatttttctg attccgtctt cagaacaaca aaaactcgtg ataactctac aacaaactat
     1741 cgtaattggc ttctgtaacc ccgtcacttt tcctaagaaa cgtgccccca tatgatgcgg
     1801 gagagataag tcatcgtata caggaaacac tgccaatcat aatatggcgt cattcagtaa
     1861 tttttatcca taatcagatt agcatatcat gattattgaa tattatgata tatagctcat
     1921 atgaatgtgt tattatctgt aagcgttaag catcacattg aataaccata ctaagctggc
     1981 taatcatcaa catttttata tgtaactcat tgaaattaat tatatgtttt ttgttgataa
     2041 ttaatatgta atgtgaaatt aatattttta catcatgagc gccatttaaa attaccatcc
     2101 tgaatatcat atgacaatat attttcacta aagcatgaat tgtatatgtc tattaactct
     2161 ggaaaattat caggattcac cttcaaattc acttgaatgt tacgcttaat ttcacccagc
     2221 agttcttcat gtgtggattt tattatccct ttctcataat cgataaatac acacatgttc
     2281 tttatgaatt caaaaccttt tcctgtatac agtttaatga atgccaccag agcaaacatt
     2341 tcaagatata gccataatgc cacgttagtt ttttgtaaag tataaaaaat tgaattcgcc
     2401 actcttttac ttattgctct tttatactgt gatcgggcaa gattcagtag cggaagctct
     2461 cgttcaataa atgaatgtga aaagactgga taaattgatg taggaaacct ttcaggtctt
     2521 atgatatttt ctatttccac cataaatcta tccatagaaa gaaagtcact ttcttttttt
     2581 attctggtat agagaccgcc tttattcctt tctgaggttt gttttcgtgc tatccccccc
     2641 aacactttct ctaattgttt atttatcttc gtataattaa atacaaccat tcttcttatc
     2701 atcctcggaa ggataataat atcagtgctt gaatcacttg agcggcaatt attatttatc
     2761 catgcaactt caagttgcag tatcggaaca ttaacttttc tggggtgaat atctctctga
     2821 tatcgttttt tgtatgcgtt ttcattaaga tattctctgc tgtagagaaa cttatagcaa
     2881 tataatctga taatatcttt tatgtaaaat ttaaatagtt cacctgtaac agatatatgt
     2941 tttctgctca gtaactcctg tgtattaagc cattcccgtg accgaagcac acccttgtaa
     3001 aaactttttc ttacttgctt tgaagcacgc cattgatgta atatttttgc gtcctcaata
     3061 attctctttc ccgttttatt ttttgcagca tctcttactc tataaaatat ctcccagtcc
     3121 agacttttgt catatctact aattatacga caaatattcc tgacccgacg attctcttta
     3181 tttcgcttcc atagcttata atgatcatcg cataacctta aggcatttgc ctcatcaaat
     3241 tctgaaacag gattactgca ttttttattc cgacagatac ctttgttttt agccatactc
     3301 ttcttcccgt caatggaaaa attttcacac ccatattacc tgaatgataa accggattag
     3361 tgtgatccgg ttcagtgaaa tcaacaggat accggtatgc cattcagcaa ttcttccctc
     3421 tccgcgcaag tgaaatcata tctgacgttt cttcctgaag aaattcgcca gaaaatcctt
     3481 gaacatctcc acggtgttat tcattacgag cccgtaattg gcattatggg taaatccggc
     3541 accggcaaaa gcagtctgtg taatgccatt tttcagtccc gtatctgcgc cacgcatccc
     3601 ctgaacggct gcacccgcca ggctcatcgt cttaccctgc agctcggtga acgcagaatg
     3661 acgctggtcg atatgcccgg cattggtgag acaccgcagc atgatcagga ataccgggag
     3721 ctttaccgtc agttactgcc ggaactggat ctgattatct ggatcctgcg ggctgatgaa
     3781 cgtgcgtatg ctgctgatat tgccatgcat cagtttttac tgaatgaagg tgcagatccc
     3841 tcgcgttttc tgtttgttct cagccatgcc gatcgcgtgt ttcctgctga agaatggaac
     3901 gacacagaaa aatgcccgtc ccgtcagcag gaactctcac tggcgacagt aacagcccgg
     3961 gtggcaaccc tgttcccgtc atcatttccg gtactctccg ttgccgcacc tgtcggctgg
     4021 aatcttccgg cactggtgtc gctgatgatc cacgcactgc caccacaggc aaccagcgca
     4081 gtttattcac atatccgggg ggaaaaccgc tctgaacagg cccggaaaca cgcacaacaa
     4141 acttttggtg acgccatcgg gaaaagcttt gacgctgccg ttgcccaatt cagctttccg
     4201 gcctggatgt tacagcttct gcgtaaggcc cgggaccgca ttatccactt gctggtcaca
     4261 ctgtgggatc gtctgttctg acacactcac gccgacagga tgtgtcgctg gattaacaag
     4321 cattcttctt tttatgaaac cctgcttaaa aagcagataa ttaaaggaat attttttctg
     4381 ctgcatttta tttctgatta ttcggatgcg acacatcctt tcaacatcat gatgcataat
     4441 aacatcatga aataaaagat gttttcttac ggagtgcaca tctatgtctg ataatcgttc
     4501 ccggcatgat cgcctggcgg ttcgcttatc actcattatc agccgactga tggccggaga
     4561 atctctgtca ctaaaaacac tgtcagatga atttggtgtt acagaacgta ccttacagcg
     4621 cgattttcat cagcgcctgg ttcacctgga cttagagtac agaaatggcc ggtacagtct
     4681 cagacgacag agcagcccag gtgcgatccc tgaaatgctt tcttttatac agaataccgg
     4741 gatcgcacgg atacttccgc tccggaacgg acgactgata acctgtctta ccgacaacca
     4801 ggagccctct ccctgcctta tctggctacc ggcgccggat atcactgcaa cgttccccga
     4861 gtgtttctcg caactcatcc tggcaataag acagtgtatc cacatctctc tgatgactga
     4921 gcgatggtat ccgtcactgg agccctgccg gctcatttat tacagcggta gctggtatct
     4981 gatcgcgtta cagaagggaa aactgcaggt ctttcctctg gcagatatca aatcagtcag
     5041 cctgacatca gaacggtttg aacggagagg ccacatccac agtctggtcg ctgaagagcg
     5101 ttttatctcc gccctgccac atttctcttt catccataaa cttatcaaca cctttaacct
     5161 gtgatcgccg gcctgccaaa gccgtcccga caggtatgga gacaatatgt tgaacagaaa
     5221 actaaatata cggctacgtc attccctgaa cagtcactgc ataccttcca tcattatcaa
     5281 taacaccgta cgttcatttc agaggtcagt catgaatacc agagctcttt ttcccctgct
     5341 gttcactgtg gcatcattct ccgcctccgc cggcaactgg gctgtcaaaa acggctggtg
     5401 tcagaccatg acggaagatg gtcaggcgct ggtaatgctg aaaaatggca cgattggtat
     5461 tactggcctg atgcagggat gcccgaatgg tgtacagacg ctcctgggca gccgtatcag
     5521 tattaacggt aacctgatcc ccacatcaca aatgtgtaat cagcagacgg gattcagggc
     5581 tgttgaggtg gaaatcggac aggcgccgga aatggtcaaa aaagccgttc actccatagc
     5641 agagcgtgat gtgtccgttt tacaggcatt tggtgtacga atggaattca cccgcggtga
     5701 tatgctgaag gtctgtccga aatttgtcac atcacttgcc ggtttttccc cgaaacagac
     5761 gaccactatt aataaagatt ccgtcctgca ggctgcccgg caggcatacg cccgggaata
     5821 tgacgaggaa acaacagaaa ccgctgattt tggctcttac gaagtaaaag gcaataaggt
     5881 tgagtttgaa gtattcaatc ctgaagaccg tgcgtacgac aaagtgaccg tcacggttgg
     5941 tgctgacggt aatgccacca gcgccagcgt tgaatttatc ggaaaatagt cggtatgtcg
     6001 gactgccacc ctgttttatt gcccgaaggc cctttctcac gcgaacaggc ggttgccgtc
     6061 acaacagctt accgcaatgt gcttattgaa gatgaccagg gaacgcattt ccggctggtt
     6121 atccgcaatg ccgaagggca gctacgctgg cggtgctgga attttgaacc tgatgccgga
     6181 aaacagctaa attcgtatct cgccagtgag ggaattctca ggcaataaac gtcttcattt
     6241 catccatcag gccgcgtctt ctccgggaga cgcggctttt tcgtttatac cgctaattca
     6301 ttcataagga gcaaagtatg caattagcca gtcgttttgg tcatgtaaat cagatccgtc
     6361 gggagcgccc actgacacgc gaagaactga tgtaccacgt cccgagtatt tttggagaag
     6421 accggcacac ctcccgcagt gaacggtatg cgtacattcc caccattacc gtcctggaaa
     6481 atctgcagcg ggaaggcttt cagccgttct tcgcctgcca gacccgtgtg cgcgaccaga
     6541 gccgcc
//
LOCUS       DAMZFT010000209         6505 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.209, whole genome shotgun sequence.
ACCESSION   DAMZFT010000209 DAMZFT010000000
VERSION     DAMZFT010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6505)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6505)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6505
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.209"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(<1..466)
                     /locus_tag="PN977_004802"
     CDS             complement(<1..466)
                     /locus_tag="PN977_004802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosome-binding GTPase"
                     /protein_id="HDI6044731.1"
                     /translation="MNPSDAIEAIEKPLSSLPYSLSRHILEHLRKLTSHEPVIGIMGK
                     SGAGKSSLCNALFQGEVTPVSDVHAGTREVRRFRLSGHGHSMVITDLPGVGESRDRDA
                     EYEALYRNILSELDLVLWLIKADDRALSVDEYFWRHILHRGHQRVLFVVTQAD"
     gene            826..1332
                     /locus_tag="PN977_004803"
     CDS             826..1332
                     /locus_tag="PN977_004803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001349675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6044732.1"
                     /translation="MECIVKKMTAPEQEEILMKPMVIALNAAIRAGRINLNGKSEDTD
                     SKGFMRTDIDGMPTVINWTDNGYDELRVSVWVDYRPDEVARFRSRYKPDLSPETALPR
                     EDRLLFRHFVMICCSCYLERKTGKFIIGTEGNRLFGKYIRDDAPERISDIEDEEPEGY
                     DLFGKVEE"
     gene            1964..2980
                     /locus_tag="PN977_004804"
     CDS             1964..2980
                     /locus_tag="PN977_004804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004237796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HDI6044733.1"
                     /translation="MYFKRTVIPATFLFASFPAFSQDIVTLYTTREPGLIKPLLDSWT
                     KSSGINVNTVYIKDGMLERIKAEGKNSPADLLMTVDAGNLIDLVEAGVTQPVESEILK
                     TAIPANLRGENNHWFALSMRARVLYAEKNFPIDSLHYEQLASPEYKGRVCMRSGQHPY
                     NTALIAAMIAHHGEEKAEEWLHGLKTNLSRKATGGDRDIARDILGGICDIGLANSYYV
                     GHMKNAKEGTDARKWGDAIKVIKPAFVQGGTHVNITGAAVARYAPNKENAIKLMEYLI
                     SVPAQQQYAKANFEYPVLKGVDLDPVIRTTIGEIDVDKIPLTDIVKNRKQASLLVDKV
                     GFDQ"
     gene            3063..4652
                     /locus_tag="PN977_004805"
     CDS             3063..4652
                     /locus_tag="PN977_004805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006684009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HDI6044734.1"
                     /translation="MALGVLTPVITLFWLATDADSSQWSGFLTFVLPSALQNTVILLT
                     GVAVMTCVLGVGCAWAVTVWDFPGRKMLSWALVLPLAMPAYIVAFAWLDLLHPLGPLQ
                     NFIRLLSGFDSPRQFRLPDLRSLPGAIILLGLVLYPYVYLTCRPVFINQSAHLLEAAR
                     TLGCGPVSAFFRVTLPLARPAIVTGMSLALLETLNDIGASEFLGIRTITVTVYTTWIS
                     RSDLAGAAQIAGIVLTFVFMILGLEFLFRRKQVFSSRSMRGIQQIRIYGWRGWLLCSI
                     TALPVILGFCAPVLFLGWESAKRLGEGITISSSLLSALKNTLFLASGTTLIVSGVSLL
                     VVWSARHSVLNSRFIEFRRTVMHLASSGYAVPGTILAIGFLPPAMLVDHWLAGLLNIS
                     GLPLMSAGILLVICCAIRFLAISTGALDSALGRIPPSLEQASRLLGENGAGTFVRVHL
                     PLLRPALISSALLVFSDVMKELPTTLLLRPVNFETLSTLLYAEAARGTYEQGAIAALL
                     IVLAGILPVILLVRRQMNSHH"
     gene            4668..5345
                     /locus_tag="PN977_004806"
     CDS             4668..5345
                     /locus_tag="PN977_004806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010281453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDI6044735.1"
                     /translation="MSEIALSLNDVHVVYTRSRQVVLQGFSMSVHKGELVCLLGVSGC
                     GKTTVLRTVAGLERLQQGEIFISQRCVAGAGIHLPPEKRRVGMVFQEYALFPHLTVTQ
                     NVAFGLRHLPYELQHARVTELLKMVELLSCAKRYPHELSGGQQQRIALARALAPAPDI
                     LLLDEPFSSLDKQTSEYLVKEVRDLLRSSGQTALIVTHNESEALEISNRICIMTEGNI
                     SSVQSVV"
     gene            complement(6154..>6505)
                     /locus_tag="PN977_004807"
     CDS             complement(6154..>6505)
                     /locus_tag="PN977_004807"
                     /inference="COORDINATES: protein motif:HMM:NF018232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1097 family protein"
                     /protein_id="HDI6044736.1"
                     /translation="PDILKKIYASLLPGIATSVLCIAAINYMISLHISAMLAIIISVI
                     ITVYVLLLLLQIPCMNSSLPAFNAYSTVFAVYYGGFYPDTDGPDISLAVLWAATGLCT
                     GPLLGYISIVCSRK"
BASE COUNT         1837 a         1479 c         1457 g         1732 t
ORIGIN      
        1 tgtcggcctg cgtcaccaca aacagtaccc gctgatgtcc ccggtgcagt atgtgtcgcc
       61 agaaatactc atccacagac agggcacggt catcggcttt aatcagccac agtaccaggt
      121 ccagttcaga cagaatgtta cggtacaggg cttcatactc tgcatccctg tcccggctct
      181 cgcccacccc gggcaggtca gtgataacca tgctgtgacc atggccactc agacggaagc
      241 gccgcacttc ccgggtgccg gcgtgaacat cactgaccgg ggtgacctcc ccctgaaaca
      301 gtgcattaca gagtgaggat ttaccggccc cgcttttccc cataatgcca atcacgggtt
      361 cgtgactggt gagtttgcgc agatgttcca ggatgtgacg ggaaagtgag taaggcaggg
      421 aggagagcgg tttttcaatt gcctcaatgg catcagacgg attcatatat gtgttcccgg
      481 tgaaaaaaga caaaaccccg cgcaccggaa aggtggcggg ggttccacgg agatgatatc
      541 tgtctgaaat tatttagaat atcactgtat tccggcgtga cagagtctga tgacggactt
      601 attccgttcc ggtcaccggc aggcacatcg gtaatacgct gttttattaa agatactgtg
      661 attagagata tgtaaataac cgctcagaat gttctgttat gtcactcacc atgaacagca
      721 caccgtaacg gtgaaagaag aacaagataa tcaacttccc cgtgattgcc atcagaaatt
      781 attttatcct tgacaatatt atatgtttta tttcggtaaa caataatgga gtgcatcgtg
      841 aaaaaaatga ctgccccgga gcaggaggaa atactgatga agcccatggt gattgcgctc
      901 aacgcagcca ttcgggccgg gagaatcaac cttaacggca aatcagaaga tacggacagt
      961 aaaggattca tgcgtacgga tattgacgga atgcccactg tcattaactg gaccgataac
     1021 gggtatgatg aactgcgtgt atcagtatgg gttgattacc ggccggatga ggtcgccaga
     1081 ttcagatcgc gttataaacc tgatctcagt cctgaaacag ccctgccccg tgaagacaga
     1141 ctgttattca gacattttgt gatgatttgc tgcagttgtt atctggagag aaaaacaggt
     1201 aagttcatca ttggcactga aggaaaccgg ctgtttggta aatatattcg tgatgacgct
     1261 ccggagagaa tttcagatat tgaagacgaa gagccggaag gatatgacct tttcgggaaa
     1321 gtcgaagaat gaacggtaag caggctcagt ctgatgaaat ggtcaggacc cacaggaaag
     1381 aagaacacgt ctcatctcac cacaggtcgt ctgacctgca cgcatgacaa aacgtcatgc
     1441 gtaagccgat gagtgaaacc gctgtcaaca gaattataaa gcgaaccgtc caggatgacc
     1501 gtgcaacaga ccatggattc catcgcactg cactttatcc ccgcccttct ggtgagcact
     1561 acgccaccgg aaggatgacg caccgaacag gaagaataat tattctttta tttaaagaat
     1621 tttatataaa acagaaacct ccatactaaa tcaataaact gagaccttca cagcatcacc
     1681 caccacccac agtgatatat aacaaaaaag atacaatcac aggcaaatac cacgctgaaa
     1741 aacattcagc acaattcaat tacgccagtg agtaatccgg taacagtatt acataccttc
     1801 ctgccagaat ctccacccac ataaattgtc aaataattgc aaaaatttac aatcaaaacg
     1861 tattgaaaat aatttctatt accatttaat aaagtactcc ctgattacct gattaagtga
     1921 gtcatcaaac atgaaacttt tcagttcaaa acagcggaca aaaatttatt tcaaacggac
     1981 tgtaatacca gcaacttttc ttttcgcatc atttcccgct ttttcacagg atattgtcac
     2041 cctttataca accagagagc ccggcctgat taagccactt ctggacagct ggacaaaaag
     2101 cagcggtata aatgttaata cggtttatat caaggacggt atgctggagc ggattaaagc
     2161 tgaaggaaaa aattctccag ccgatctact gatgaccgtc gatgccggaa atctcattga
     2221 tttagtcgaa gcgggagtca ctcagcctgt tgaatctgaa atactgaaaa cggccattcc
     2281 ggcaaatctt cgtggagaaa ataaccactg gtttgcttta tccatgcggg cccgggtact
     2341 gtatgcagaa aaaaacttcc ccattgacag cctgcattat gaacaactcg ccagccctga
     2401 atacaaaggc agggtctgca tgcgttcagg gcagcaccct tataatacag cattgattgc
     2461 tgccatgatc gcccaccacg gagaagaaaa agcagaggag tggttacacg gtcttaaaac
     2521 caacctgtca cgaaaagcaa cggggggcga tcgcgacata gcccgcgata ttttaggagg
     2581 gatatgtgac atcggacttg cgaattctta ttacgtcggt catatgaaaa atgccaaaga
     2641 agggacagat gcacgcaaat ggggagatgc aattaaagta atcaaaccag ccttcgtgca
     2701 gggagggacc catgtcaaca taaccggcgc agccgttgct cgttatgcac cgaacaaaga
     2761 aaatgcaata aaactgatgg agtatctcat atctgttccg gcgcagcagc aatatgccaa
     2821 agctaacttt gagtacccgg tactcaaagg cgttgatctt gaccctgtca tcagaaccac
     2881 tattggtgaa atagatgtcg ataaaattcc gttaactgac atcgtaaaga accgcaaaca
     2941 ggcaagctta ctggtagata aagttgggtt cgatcaataa atcattttgg gctttagtgt
     3001 ggcgttatct tttttctgca ttacgttcca cgctaaagcc tttacatctg tgctcgctgt
     3061 gtatcgcact gggtgttctt acacctgtca taacgttatt ctggctggca acagatgcag
     3121 acagttcgca atggagcggt tttttaacgt ttgtgctacc ttccgctctg cagaataccg
     3181 ttattctgtt aacgggtgtt gctgtaatga catgcgttct tggtgtgggg tgcgcctggg
     3241 cagtgactgt ctgggatttt cccggccgaa aaatgctcag ctgggcatta gtgttacctc
     3301 tggccatgcc tgcctatatt gttgcttttg catggctcga tctcctgcat cccctggggc
     3361 ctctgcaaaa ttttatccgt cttttatcgg gatttgacag tccccgtcag tttcgtctgc
     3421 cggatttgcg ctcccttccc ggagcgataa tattactcgg tcttgtactg tatccttatg
     3481 tctacctgac ctgccgaccg gtgtttatca atcagtccgc tcacttactg gaggcagcac
     3541 gaacattagg ttgtggccct gtctctgctt ttttccgggt aacgctacct ctggcacgcc
     3601 cagcaatagt gacaggtatg agtctggccc ttctggaaac cctgaacgac attggtgcat
     3661 ctgaatttct cggtatccgg acaatcacag taacagtcta caccacatgg atatcacgct
     3721 ctgatcttgc cggtgctgca caaattgccg gcatagttct gacatttgtc ttcatgattc
     3781 tgggtcttga atttttgttc cggagaaaac aggtattcag tagtcgcagt atgcggggaa
     3841 tacaacaaat caggatttat ggctggcgtg gatggttgct gtgcagcatt acagcactgc
     3901 ccgttattct gggattttgc gcacctgtat tattcctggg atgggaaagt gcaaagcgtc
     3961 tcggagaagg gattacgata tcttcatcat tgctgtcagc actgaaaaat acattgtttc
     4021 tcgcatccgg tacgaccctg attgtatcag gggtcagttt actggtggtc tggagcgcac
     4081 ggcacagtgt actgaacagc cgtttcatcg aattccgacg aacggtcatg cacctggcat
     4141 cttcaggata tgctgttccc ggtactattc ttgctatcgg ctttctgccg cctgcaatgc
     4201 tggttgatca ctggctcgct ggtcttctta atatcagcgg ccttcccctc atgtctgcag
     4261 gtatactgtt agttatttgc tgtgcgattc gtttcctggc catttcaacc ggagcgcttg
     4321 attcagccct tggtcgcata ccgccgtcac tggaacaggc atcacgatta ctgggagaaa
     4381 atggcgcagg gacttttgtc agagttcatt tgccattact tcgccctgct ctgatcagca
     4441 gtgcgctgct ggttttctcc gacgtgatga aagaactgcc gaccacctta ctgctacgac
     4501 ctgttaactt tgagaccctg tccacactcc tgtatgcgga agcggcacgt ggaacctacg
     4561 aacagggcgc aattgctgca ctgttaatcg tacttgccgg catactccct gtgattttat
     4621 tagtacgcag gcaaatgaac agtcaccact aaaggaaccc aaccaccatg tcagaaattg
     4681 ccctgagtct taatgatgtt catgttgttt ataccagaag cagacaggtc gtgttacagg
     4741 gtttttcaat gtccgttcat aaaggtgaac ttgtatgtct gcttggcgtt tcaggttgtg
     4801 gaaaaacaac cgtattacga actgtagcag gcctggaacg attacaacaa ggtgaaatat
     4861 tcatatcaca acgctgtgta gccggagccg gaattcatct ccccccggaa aagcgcaggg
     4921 tcggaatggt ctttcaggaa tatgctctgt ttccccacct gacagtaaca caaaatgtag
     4981 cctttggtct gcgccatctg ccttatgaac tacagcatgc cagagtcact gaacttctga
     5041 aaatggttga attattatcc tgtgcaaagc gctatcctca tgaactctca ggtggacagc
     5101 aacaacgtat tgcacttgcc agagcactgg ccccggcccc tgatattttg ttactggatg
     5161 agccattttc cagtctggat aaacaaacca gtgaatacct agtgaaagaa gtacgggacc
     5221 tgttacgtag ttcaggtcag acagccctga tcgttactca taatgaatca gaagcactcg
     5281 aaatatcaaa ccgtatttgt atcatgacgg agggtaatat aagttcggtt cagtctgtgg
     5341 tctgagacgt aaccgcacat tcaggatccc ctgttcgtca tggaagctgt attatgtaac
     5401 tatatggagg catagcagat attgttgaag aaatacatca aaaacactca catttctggt
     5461 acaacaggag ttgtagattt aatgattatt gggacgaagt tactacagaa aagctgtacc
     5521 gggaacaggt atggtagttc cttgtcacgt gtggattaaa tgagcataat gcagatcaat
     5581 tacaaaaggc atttcgtaat agctctgttg ttgattcggt taccatgatg gctgtggcaa
     5641 gagccaccat gtgactatac atgatataat tatttgtgtg atgttgttat acctgaacaa
     5701 caggcatgag ataatggaaa aaattataca tataactcgg ctttcccata ctccactaca
     5761 gaacagtata tgattctgaa catttcaatc caggcgcatt acataactat attacatgta
     5821 actatctggc cctcaaatca tcgagagaat tgtttaaagt cagcagtcat aatctgaata
     5881 tagatcatta tcacataatt taattcttaa ttcactacat tttcccttga atccggtata
     5941 ataagacaga gcacatggct gatgctttgt ttcggtgtgg ataatctggt ggcgtcggct
     6001 atgtgctctc aacagttaac ttattatgaa tactattatc aatacaggtt ttacataaaa
     6061 aaataaaaag catctcctga tatcagccca gtctctttgg gctaaccaga attatacata
     6121 agctatgcag atattaattt gcatacaaaa ccattatttt cttgagcaca ctatactaat
     6181 gtatcccaga agaggtcctg tgcataatcc tgttgctgcc cataatacag caagtgaaat
     6241 atcaggacca tctgtatccg gataaaatcc tccataataa acagcaaata cagtagagta
     6301 tgcattgaat gcaggtaaac tactgttcat gcacggtatc tgaaggagta aaagaagaac
     6361 atacactgtt attattactg atataatgat tgccaacatg gcagaaatgt gcagggatat
     6421 catgtaattt atagcagcaa tacataacac agacgttgct ataccgggaa gtaatgaagc
     6481 ataaattttc tttaatatgt caggc
//
LOCUS       DAMZFT010000210         6481 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.210, whole genome shotgun sequence.
ACCESSION   DAMZFT010000210 DAMZFT010000000
VERSION     DAMZFT010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6481)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6481)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6481
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.210"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(<1..1380)
                     /locus_tag="PN977_004808"
     CDS             complement(<1..1380)
                     /locus_tag="PN977_004808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000086499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HDI6044737.1"
                     /translation="MSRIESDFLGEREIPDDCYYGVQTLRGKDNFHITEMPMSQEPFF
                     IIAFGYVKKAAAMANKELGTIPADVADALIWACDQLIDGKYREQFVTDWLQGGAGTST
                     NMNCNEVICNLAAEKLGSVKGDYKRVSPNDHANFGQSTNDTYPTALHLALLLRSNVLL
                     EAVEHLVGAFYKKADEFSTVLKMGRTHLQDAVPMTLGQEFHGWGFTINDEIRVIRNAQ
                     EHLRVVNLGATAIGTCVTAHPDYPALAVKYLAQITGINFRNSEDLIAATSDCGAYVAL
                     SSAMKSLSVKLTKVCNDIRLLASGPRCGLAEINLPQLQPGSSIMPGKVNPVIPEVTNQ
                     SCFLVQGLDTTVMLAASAGQLELNVMEPVITFALFTSLKVMTNACNTLRTKCIDGITA
                     NSDRTAEMVMHSCGIVTLLKPHLGYKVCSEMAHEAYHTGKSLHQIVVVERKLLTQEEW
                     EKTFNLDNLI"
     gene            complement(1603..2901)
                     /locus_tag="PN977_004809"
     CDS             complement(1603..2901)
                     /locus_tag="PN977_004809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001616045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter"
                     /protein_id="HDI6044738.1"
                     /translation="MVWLEIIVVLGAIFFGIRQGGIGIGLCGGLGLAILTLGFGLPMG
                     SPPVDVILIIMTVVVAASALQAAGGMDYLVRLASNFMRRNPKYINIIAPIITWLMTIM
                     AGTGFIVFSTLPVIAEVAKESGIRPSRTLAGSVVASQVAISGSPISAAMAAMLTIMEV
                     NGVSFIQVMSVCLPTSFVAAMVAAFIASRQGCELQDDEVYLERLQKGLVQKYENNNSI
                     KPGATLSVGLFMLATIAIVILAAFPQLRPGFDISKPMETRDIIIICMLSAACLMVILC
                     KMSTDDIILTSTFRAGMSSLAVILGIVTLGTTFIDAHLTEIKDIAGDILQTYPMLLAL
                     VLFFTCALLYSQGATTPLIIPLAVALNIPTWAILASYVAVTGVFVLPTYPTSLAAMEF
                     DTTGTTRVGNYLLNHPFMLPGLGGVIAGVTFGFVIAPMMV"
     gene            complement(2960..3679)
                     /locus_tag="PN977_004810"
     CDS             complement(2960..3679)
                     /locus_tag="PN977_004810"
                     /EC_number="5.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016156986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HDI6044739.1"
                     /translation="MKGLIGVLGGMGPAATVDLFNKFVTFTAAQRDQEHIPLIISSIP
                     DIPDRTDALMHNGLSPLPVMRDYMHKLEDAGAECIVIPCNTAHYWFKELKDACHIDIL
                     SIIDTTVDEVRVCGKTRIGLLATDATLYMGLYQKGIESLGFNYITPDADGQKNVMKSI
                     YSLKAGNVTHAQKLMEEQAENLFSRGAEILILGCTEIPIILSQAVKDQPLRYIDSTAS
                     LVRAGIKWYENRIGKDQHLTQ"
     gene            4019..4918
                     /gene="hypT"
                     /locus_tag="PN977_004811"
                     /gene_synonym="qseD"
     CDS             4019..4918
                     /gene="hypT"
                     /locus_tag="PN977_004811"
                     /gene_synonym="qseD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000789106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypochlorite stress DNA-binding transcriptional
                     regulator HypT"
                     /protein_id="HDI6044740.1"
                     /translation="MKNIETKWLYDFLTLEACRNFCQAAKDRNLSQPAFSRRIKALES
                     AIGVVLFDRTTSPLQLTEEGRLFHSQTRSLVQQLECNLNELGGDNSFNLPNIKIAAAH
                     SLSLSMLPKLLHSLTTLGGDFIYHVEAIDVVQAVNTLREGKSDFIISFRDEEFMQSPF
                     CCMKLFESELYPVCAADQQGKPIFDLTNSQAPLLDYTPTSYMGRLVNRHLAEQGGIAG
                     HTILVSSMSELLKNMALNGLGIAWLPLWSIADELNTKRLVCPWPAKWAVPIQAYIYRM
                     DTRLNKTAEHFWTILRNNIKENS"
     gene            5578..6078
                     /gene="tnpA"
                     /locus_tag="PN977_004812"
                     /pseudo
     CDS             5578..6078
                     /gene="tnpA"
                     /locus_tag="PN977_004812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459923.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            complement(6272..>6481)
                     /locus_tag="PN977_004813"
     CDS             complement(6272..>6481)
                     /locus_tag="PN977_004813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDI6044741.1"
                     /translation="SSLAPMRLIGLTGGVFNFVGGLGGITVPLVVGYLAQGYGFAPAL
                     VYISAVALIGALSYILMVGDVKRVG"
BASE COUNT         1951 a         1386 c         1410 g         1734 t
ORIGIN      
        1 aatcagatta tccagattga atgtcttctc ccattcttcc tgtgtgagta gcttacgttc
       61 gacaaccact atctgatgga gggatttgcc tgtatggtat gcttcgtgcg ccatttcaga
      121 acacacctta tatcccagat gtggcttcag gagagtcaca ataccgcagg aatgcattac
      181 catctctgca gttcgatcgg aattagctgt aatgccatca atgcatttag ttcggagtgt
      241 gttacaggca ttcgtcatca ccttgagtga ggtgaacagc gcaaaggtga tgaccggctc
      301 cataacgtta agctcaagct gaccagcaga tgccgccagc atcactgtgg tgtccagccc
      361 ctgaaccagg aagcaagact ggtttgttac ttccgggata acggggttaa ccttaccagg
      421 cataatggaa gaacccggtt gcaattgagg caggttaatt tcagccaggc cgcaacgggg
      481 gcctgaagca agcagtcgaa tgtcattaca aaccttggta agcttcacag agaggctctt
      541 catggccgaa ctgagtgcaa catatgcacc acagtcgctt gtggcagcga tgagatcttc
      601 actgttcctg aaattaatgc ctgtgatctg ggccagatat ttcacggcta aagcgggata
      661 atccgggtga gcggtaacac aggtaccaat cgcagtagca ccaaggttta caacccgcag
      721 gtgttcctgt gcattacgga tgacccgaat ttcatcatta attgtaaaac cccagccatg
      781 gaactcctgg ccgagagtca ttggcactgc atcctgcagg tgagtacgtc ccatcttgag
      841 cactgtgctg aactcgtcag cctttttgta gaatgctcct acgaggtgtt caacagcttc
      901 aagcaataca ttgcttcgca gcagcaatgc cagatgtaat gctgtcggat atgtatcatt
      961 ggtggactga ccaaaattag catggtcgtt tggcgaaacg cgtttgtaat cccctttaac
     1021 gctaccaagt ttttctgcag caagattaca aatcacttca ttgcagttca tattggttga
     1081 cgttccggct cccccctgaa gccagtcggt cacaaactgt tcccggtact taccatcaat
     1141 taattggtcg caagcccaga ttaaagcatc cgcaacatca gcaggaattg tacctaactc
     1201 cttattagcc atcgcagctg cttttttcac ataaccgaag gcaatgatga agaaaggctc
     1261 ctgactcatt ggcatttcag tgatatggaa attatctttt ccacgaagtg tctgaacacc
     1321 ataatagcaa tcatccggaa tttcacgctc accaaggaaa tcgctttcaa tacgagacat
     1381 aatattctaa ctccattatt tgaaaacaat gtgacaataa caaattgttt aaccggagct
     1441 atgctctcgg aatttgaatt cttacatcat tgaaataaca tgtgatatca atctgtcaga
     1501 attcttctga cagtcagtaa ctgaatgcag attaactgcc tgactaatta tcaaatagta
     1561 actctgtaac tacttccata aagaatgaaa attatgattc tatcagacca tcattggtgc
     1621 aatcacaaac ccaaatgtca ctcctgcaat aacaccaccc aacccgggta gcataaatgg
     1681 gtgatttaat aaataattgc ccacacgggt tgtgccggta gtatcaaatt ccatcgcggc
     1741 gagtgatgtt ggatacgttg gaagcacgaa tactcccgta acggcaacat aagatgcaag
     1801 aattgcccag gttggtatgt tgagtgctac agccagagga ataattaacg gcgtagtcgc
     1861 tccctgtgag taaagtaaag cgcaggtaaa aaaaagaact aatgctaaaa gcatcggata
     1921 agtttgtaaa atatcaccag cgatatcttt gatctcagtt aaatgcgcat caataaaggt
     1981 ggtacctaag gttacaatac caagaatcac tgccagcgaa ctcattccag cacgaaatgt
     2041 ggaggtcaga ataatgtcat cagtagacat cttacacagt attaccatca ggcaggcagc
     2101 agagagcatg caaataataa taatatcgcg cgtttccatc ggtttgctga tatcaaaacc
     2161 cggacgcaat tgggggaatg cagcaagaat aacaatggcg atcgtcgcca gcatgaataa
     2221 tccgacggaa agcgttgcac caggtttgat gctgttatta ttctcgtatt tttgcaccag
     2281 acctttttgc agacgttcaa gatatacttc gtcatcttgt aattcacacc cctgtcggga
     2341 ggcgataaaa gcagcaacca tcgcggcgac aaaagaggtg ggaaggcata ccgacatcac
     2401 ctgaataaaa ctgacgccat taacctccat aatagtgagc atagctgcca ttgcggcact
     2461 gattggtgag ccagaaattg caacctgtga ggcgacaact gagccagcga gtgtgcggga
     2521 aggccggata ccggactctt ttgctacttc agcaattacc ggcagtgtgg agaaaacaat
     2581 aaacccggta ccggccataa tggtcattaa ccaagtgata atcggtgcga ttatgttgat
     2641 atattttggg ttacgtcgca tgaaattact ggctaaacgt accagataat ccatcccacc
     2701 tgccgcctgt aaggctgaag ctgccacaac tacggtcata ataatcagga tcacatcgac
     2761 tggcggtgat cccataggca gaccaaatcc cagagtcagg atcgcaaggc caagaccgcc
     2821 gcataaacca atgccgattc ctccctgacg aataccaaaa aatattgcgc caagtacaac
     2881 gataatttcc agccaaacca tgatgttctc cgttgtaaat ataatctttg taaaataact
     2941 ttctggctgt gatttagaat tattgagtta aatgttgatc ttttccgata cgattttcat
     3001 accattttat tcctgcgcga acgagtgaag cggttgaatc aatatagcgt aaaggttgat
     3061 ctttaacggc ttgtgacaga ataatgggaa tttccgtaca accaagaatc aagatctctg
     3121 ctccgcggga aaataaattt tcagcctgct cttccatcaa tttttgcgca tgtgtaacat
     3181 ttcccgcttt aagtgaataa atgcttttca tcacattttt ttggccatct gcatccgggg
     3241 tgatatagtt aaaccctaac gattcaattc ctttctggta taatcccata tacagcgtgg
     3301 catcagtggc caggaggcca attctagttt taccacagac gcgaacttca tcgaccgtag
     3361 tatctatgat acttagtata tcaatatggc aagcatcttt tagttcttta aaccaatagt
     3421 gtgcggtatt acagggaata acaatacatt ctgctcctgc atcttccagt ttatgcatat
     3481 agtcacgcat aacgggtaaa ggcgagagcc cgttatgcat taatgcatcg gtacggtcag
     3541 gaatatccgg gatagaagaa ataattaaag gtatatgttc ttgatctcgt tgtgctgctg
     3601 taaaggtgac aaatttatta aataagtcga cagtggcggc cggccccatt ccaccgagca
     3661 caccgattaa acctttcaca atacttacct ctcagaaaat caatgctcat aaacttaaaa
     3721 aaccatttct ccttttttaa cagtctctaa aggctatgag aaatgcaatg atgtacaatg
     3781 caatgcaaga cttgcatgat gtagttttgc aatatacatg aaccatatac tattgttgca
     3841 tgaagaccta tcataatgat gaaaataaac gcttttggga gaagtgttaa actggtttag
     3901 atacagaaaa cgaatggatt atgcataaaa cgaataactt aaatatgcta tctgcattca
     3961 ttaccccggt tttcgggggg tatactaact aaaatttgat aatacttgga taatagaaat
     4021 gaaaaatata gagaccaaat ggttatatga tttcctgaca ctggaagcct gtcgcaattt
     4081 ctgtcaggca gcaaaagatc gtaacctttc acaaccagca ttcagcagac gaataaaagc
     4141 gttagagtca gctataggtg ttgttttgtt cgatcgtaca acctctcctt tacaattaac
     4201 tgaagagggt aggctttttc actcccagac acgcagttta gtgcaacagc ttgaatgcaa
     4261 tctgaatgag ttaggggggg ataattcatt caatttgcct aatattaaga ttgcagctgc
     4321 gcactcatta tccctcagca tgctacctaa acttctgcac tcactcacca ccttaggcgg
     4381 agactttatc tatcatgttg aggctattga tgtcgtccag gccgtaaata cgttacgaga
     4441 agggaagagt gactttatta tttcatttcg agatgaggag tttatgcaat cgccattttg
     4501 ttgtatgaaa ctgtttgaat ctgagttgta tccggtatgt gctgctgatc aacagggaaa
     4561 accgattttc gatcttacca attcacaggc tcctttgctc gactatactc caacttctta
     4621 tatgggcaga ctggttaatc gtcatttggc agaacaggga gggatagccg gacacacaat
     4681 attggtatct tcaatgagtg aactactaaa aaatatggca ctcaatggac ttggtattgc
     4741 ctggctccct ctctggtcaa tcgcggatga actgaacaca aaacgacttg tgtgtccgtg
     4801 gcctgctaaa tgggcagtgc ctattcaggc ttatatttat cgtatggata ctcggctgaa
     4861 caaaacggct gaacactttt ggacgatttt acgaaataac attaaagaga atagctagaa
     4921 acatcgtgga cagtttttgt tgagcacgac aaatccacgt ttttctgggg gggaaggggg
     4981 ggaattcgtt aaactggatg tcttttgctt taatcagttc atctccttca ggataattct
     5041 ggccattgag atcactttga gaggtatttt tttctctttt cacatagtaa ataataacat
     5101 tgtttaatta ataacagaaa atatactaaa atcaataatt gtaataataa attatttgtt
     5161 gtcccagata agttaagtgt cattaaaatg ctggacaatc ctcgcgtaac ggtatttaat
     5221 gactacagca cgttccagga aaagcagtgg aagcaaatat tggtcattat gtcgaaaaca
     5281 ccggaaacgt actctatatt gtgtagtcat ttattcaaat gacagcgtat aaacaaattg
     5341 atcagaatca catttttttg tgaaaatcat tgtcgagagg caacatttca tagtaagtgt
     5401 atcctacagg tcatgtataa aacctacatc atgtaaacaa actcatcgaa ttgcacctcc
     5461 tgtttgtgga ggtgcttttt tgtgcacagc tccccagcca ggctgggggt tccggaaagc
     5521 tttcagtttt gagccagtta ttaaaacctc ttttgatttg ttaaaacacc ttgcggtatg
     5581 gcaacagcaa gtgtcaaaca aaaaatcaaa agggggaccc aatggggaac gaaaagagct
     5641 agcgcacccc gatggaactg taaatatcac atagtttttg cgccaaaata ccgaagacaa
     5701 gtgttctaca gagagaagcg tagagcaaca ggcagtattt tgagaaagct gtgtgagtgg
     5761 aaaagcgtac ggattcttga agctgagtgc tgtgcagatc atatccatat gcttgtggag
     5821 atcccgccca aaatgagcgc atccggcttt atgggatatc tgaaagggaa aagcagtctg
     5881 atgctttacg agcagtttgg tgatttgaaa ttcaaataca ggaacaggga gttctggtgc
     5941 agtgggtact aagtcgatac ggtgggtaag aacacggcga agatacagga ttacataaag
     6001 caccagcttg aagaggataa aatgggagag cagttatcga ttccctacct gggcagcgcg
     6061 tttatggacc gtgagtaacg aagttggatg caaatgtcag atcgtgtgcg cctgttaggg
     6121 cgcggctggt aagagagcct tataggcgca tttgaaaaac ctccggctat gccggaggat
     6181 atttatttct tcccaccagg tgagatggtt actgaatatt tctatctgtt cagcacaggt
     6241 atgagaaaaa agggcttcct cagataagag attatccaac gcgcttcaca tcgcccacca
     6301 tcagaatgta agagagcgcg ccaatcaacg cgacggcgga gatataaacc agtgcaggtg
     6361 cgaaaccgta accctgcgcc aggtacccca ccaccagcgg aacggtgatg ccgcccagac
     6421 cgccaacaaa gttaaatacg ccaccggtta aaccaatcag gcgcatcggt gccagagaag
     6481 a
//
LOCUS       DAMZFT010000211         6434 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.211, whole genome shotgun sequence.
ACCESSION   DAMZFT010000211 DAMZFT010000000
VERSION     DAMZFT010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6434)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6434)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6434
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.211"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            307..1896
                     /gene="purH"
                     /locus_tag="PN977_004814"
     CDS             307..1896
                     /gene="purH"
                     /locus_tag="PN977_004814"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HDI6044742.1"
                     /translation="MQQRRPVRRALLSVSDKAGIVEFAQALSARGVELLSTGGTARLL
                     AEKGLPVTEVSDYTGFPEMMDGRVKTLHPKVHGGILGRRGQDDTIMEEHQIQPIDMVV
                     VNLYPFAQTVAREGCSLEDAVENIDIGGPTMVRSAAKNHKDVAIVVKSSDYDAIIKEI
                     DANEGSLTLETRFDLAIKAFEHTAAYDSMIANYFGSMVPAYHGESKEAAGRFPRTLNL
                     NFIKKQDMRYGENSHQQAAFYIEENVKEASVATATQVQGKALSYNNIADTDAALECVK
                     EFAEPACVIVKHANPCGVAIGNSILDAYDRAYKTDPTSAFGGIIAFNRELDAETAQAI
                     ISRQFVEVIIAPSASEEALKITAAKQNVRVLTCGQWGERVPGLDFKRVNGGLLVQDRD
                     LGMVGAEELRVVTKRQPSEQELRDALFCWKVAKFVKSNAIVYAKNNMTIGIGAGQMSR
                     VYSAKIAGIKAADEGLEVKGSSMASDAFFPFRDGIDAAAAAGVTCVIQPGGSIRDDEV
                     IAAADEHGIAMLFTDMRHFRH"
     gene            1908..3197
                     /gene="purD"
                     /locus_tag="PN977_004815"
     CDS             1908..3197
                     /gene="purD"
                     /locus_tag="PN977_004815"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HDI6044743.1"
                     /translation="MKVLVIGNGGREHALAWKAAQSPLVETVFVAPGNAGTALEPALQ
                     NVAIGVTDIPALLDFAQNEKIDLTIVGPEAPLVKGVVDTFRAAGMKIFGPTAGAAQLE
                     GSKAFTKDFLARHQIPTAEYQNFTEVEPALAYLREKGAPIVIKADGLAAGKGVIVAMT
                     QEEAEAAVHDMLAGNAFGDAGHRIVIEEFLDGEEASFIVMVDGEHVLPMATSQDHKRV
                     GDKDTGPNTGGMGAYSPAPVVTDEVHQRTMERIIWPTVKGMAAEGNTYTGFLYAGLMI
                     DKQGNPKVIEFNCRFGDPETQPIMLRMKSDLVELCLAACEGKLDEKTSEWDERASLGV
                     VMAAGGYPGDYRTGDVIHGLPLEEVSDGKVFHAGTKLADDEQVVTSGGRVLCVTALGH
                     TVAEAQKRAYALMTDIHWDDCFCRKDIGWRAIEREQN"
     gene            complement(3194..4519)
                     /gene="zraR"
                     /locus_tag="PN977_004816"
     CDS             complement(3194..4519)
                     /gene="zraR"
                     /locus_tag="PN977_004816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent response regulator
                     transcription factor ZraR"
                     /protein_id="HDI6044744.1"
                     /translation="MTHDNIDILVVDDDISHCTILQALLRGWGYNVALANSGRQALEQ
                     VREQVFDLVLCDVRMAEMDGIATLKEIKALNPAIPVLIMTAYSSVETAVEALKTGALD
                     YLIKPLDFDNLQATLEKALAHTHSIDAETPAVSASQFGMVGKSPAMQHLLSEIALVAP
                     SEATVLIHGDSGTGKELVARAIHASSARSEKPLVTLNCAALNESLLESELFGHEKGAF
                     TGADKRREGRFVEADGGTLFLDEIGDISPMMQVRLLRAIQEREVQRVGSNQTISVDVR
                     LIAATHRDLAAEVNAGRFRQDLYYRLNVVAIEVPSLRQRREDIPLLTNHFLQRFAERN
                     RKAVKGFTPQAMDLLIHYDWPGNIRELENAVERAVVLLTGEYISERELPLAIASTPIP
                     LVQSQDIQPLVEVEKEVILAALEKTGGNKTEAARQLGITRKTLLAKLSR"
     gene            complement(4516..5892)
                     /gene="zraS"
                     /locus_tag="PN977_004817"
     CDS             complement(4516..5892)
                     /gene="zraS"
                     /locus_tag="PN977_004817"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     ZraS"
                     /protein_id="HDI6044745.1"
                     /translation="MRFMQRSKDSLAKWLSAILPVVIVGLVGLFAVTVIRDYGRETAA
                     ARQTLLEKGSVLIRALESGSRVGMGMRMHHAQQQALLEEMAGQPGVRWFAVTDEQGTI
                     VMHSNSGMVGKQLYSPQEMQQLHPGDEEAWRRIDSADGEPVLEIYRQFQPMFAAGMHR
                     MRHMQQYAATPQAIFIAFDASNIVSAEDREQRNTLIILFALATVLLASVLSFFWYRRY
                     LRSRQLLQDEMKRKEKLVALGHLAAGVAHEIRNPLSSIKGLAKYFAERAPAGGEAHQL
                     AQVMAKEADRLNRVVSELLELVKPTHLALQAVDLNTLINHSLQLVSQDANSREIQLRF
                     TANDTLPEIQADPDRLTQVLLNLYLNAIQAIGQHGVISVTASESGTGVKISVTDSGKG
                     IAADQLEAIFTPYFTTKAEGTGLGLAVVHNIVEQHGGTIQVASQEGKGATFTLWLPVN
                     ITRKDPQG"
     gene            6130..>6434
                     /locus_tag="PN977_004818"
     CDS             6130..>6434
                     /locus_tag="PN977_004818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="periplasmic heavy metal sensor"
                     /protein_id="HDI6044746.1"
                     /translation="MKRNTKIALVMMALSAMAMGSTSAFAHGGHGMWQQNAAPLTSEQ
                     QTVWQKIHNDFYAQSSVLQQKLVTKRYEYNALLAANPPDSSKINAVAKEMETLRQSL"
BASE COUNT         1488 a         1813 c         1721 g         1412 t
ORIGIN      
        1 cgcaggccgc aatagaaaaa ttgcagaaaa atgactgact gctgcattcc ccagcaaaac
       61 cccgctttat acctttttac gcacagagtt atccacaatc atcaatgtaa tttctgtatt
      121 tttcccacgg taaacactgt caaaattgtg atcaccattg aaagagaaaa attcgcgagc
      181 gttgcgcaaa cgttttcgtt acaatgcggg cgaaaaataa ggatgccccg ttaggggcgt
      241 tagctgagtt tttcgcgaaa aattcagcta acgctctctg taatagtcaa atccagggga
      301 tttaccatgc aacaacgtcg tccagtccgc cgcgctctgc tcagtgtttc tgacaaagcc
      361 ggtatcgtcg aattcgccca ggcactttcc gcacgcggtg tggagctgct gtctacaggg
      421 ggcactgccc gtctgttagc agaaaaaggt ctgccggtaa ccgaagtttc cgattacacc
      481 ggtttcccgg agatgatgga tggacgcgtg aagaccctgc atccgaaagt acatggtggc
      541 attctgggcc gtcgcggcca ggacgatacc attatggaag aacatcagat ccagcctatc
      601 gatatggtgg ttgttaacct gtatccgttc gcccagaccg ttgctcgtga aggctgctcg
      661 ctggaagatg cggttgagaa catcgatatc ggcggcccga cgatggtgcg ctctgccgcc
      721 aagaaccata aagatgtcgc catcgtggtg aagagcagcg actacgacgc cattattaaa
      781 gagatagatg ccaatgaagg ctccctgact ctggaaactc gctttgacct tgccatcaaa
      841 gccttcgaac acaccgccgc ctacgacagc atgattgcca actacttcgg cagcatggtt
      901 ccggcttacc acggtgaaag caaagaagcc gccggtcgct tcccacgcac gctgaacctg
      961 aacttcatta agaagcagga tatgcgttac ggcgagaaca gccaccagca ggctgccttc
     1021 tatatagaag agaatgtcaa agaagcctcc gttgctaccg caacccaggt tcagggtaaa
     1081 gccctctctt ataacaacat cgccgatacc gatgcggcgc tggagtgcgt gaaagagttc
     1141 gccgagccgg catgtgtgat tgtgaagcac gccaaccctt gcggcgtggc tatcggcaat
     1201 tccattcttg atgcttacga tcgcgcgtac aaaaccgacc cgacctccgc attcggcggc
     1261 attatcgcct ttaaccgcga gctggatgcg gaaaccgcgc aggccatcat ttctcgtcag
     1321 tttgttgaag tgattattgc gccgtccgcc agcgaagaag ccctgaaaat caccgccgcc
     1381 aaacagaacg tacgcgtcct gacctgcggt cagtggggcg agcgtgttcc gggtcttgat
     1441 ttcaaacgcg tgaacggcgg tctgctggtt caggatcgag acctggggat ggtcggtgca
     1501 gaagaactgc gcgtcgtcac caaacgtcag ccgagcgaac aggaactgcg tgatgcgctg
     1561 ttctgctgga aagtggcgaa gttcgtgaaa tccaatgcta tcgtctatgc caaaaacaat
     1621 atgaccatcg gtattggcgc aggccagatg agccgtgtgt actccgcgaa aatcgccggt
     1681 attaaagcgg ccgatgaagg cctggaagtg aaaggttcct cgatggcttc tgacgcattc
     1741 ttcccgttcc gcgacggtat tgatgccgcc gccgctgcgg gtgtgacctg tgtaatccag
     1801 cctggcggtt ctatccgtga tgacgaagta attgccgccg ccgacgagca cggtattgca
     1861 atgctcttca ccgacatgcg ccacttccgc cattaatgga gcaatagatg aaagtattag
     1921 tgattggtaa cggcgggcgc gagcacgcgc tggcctggaa agcggcccag tcgccgctgg
     1981 ttgaaactgt ttttgttgct ccgggtaacg caggcactgc gctggagcct gcactgcaaa
     2041 acgtcgctat cggcgtaacc gatatcccgg cgctgctgga tttcgcgcaa aacgaaaaga
     2101 tcgatctaac catcgtcggc ccggaagcgc cgctggtgaa aggcgtggtc gataccttcc
     2161 gcgccgccgg gatgaaaatc ttcggcccaa ccgcaggtgc cgcgcagctg gaaggctcga
     2221 aagcgtttac caaggatttc ctggcccgcc atcagatccc tacggcggaa taccagaact
     2281 tcaccgaggt agaacctgcg ctggcgtatc tgcgtgagaa aggcgcgcca atcgtcatta
     2341 aagctgacgg tctggctgcc gggaaaggcg ttatcgtggc gatgacgcag gaagaagcgg
     2401 aagcggctgt tcacgatatg ctggcgggca acgcttttgg cgacgcgggg catcgcatcg
     2461 ttatcgaaga gttcctcgac ggcgaagaag cgagctttat cgtgatggtg gacggtgagc
     2521 atgtgctgcc gatggccacc agccaggatc acaaacgcgt aggcgataaa gataccgggc
     2581 cgaacaccgg cgggatgggc gcttactccc ctgcgccagt agtgaccgat gaggttcatc
     2641 agcgcacgat ggaacgaatc atctggccaa ccgtgaaagg catggcggca gaaggcaaca
     2701 cctacactgg ttttctctac gcgggcctga tgatcgacaa acagggcaat ccgaaggtta
     2761 ttgaatttaa ctgccgcttt ggcgatccag aaacccaacc gattatgctg cgcatgaagt
     2821 ccgatctggt tgaactctgc ctggcagcct gtgaaggcaa gctggacgag aaaacgtccg
     2881 agtgggatga acgcgcttct ctcggcgtgg tgatggctgc gggtggatat ccgggcgatt
     2941 accgcaccgg cgatgtgatc cacggcctgc cgctggaaga agtgtctgac ggcaaagtgt
     3001 tccacgcggg cacgaaactg gcggatgacg agcaggtagt gaccagcggc gggcgcgtac
     3061 tgtgcgtcac cgcgctgggt cataccgtgg cagaagcgca gaaacgcgct tatgccttaa
     3121 tgaccgatat ccattgggac gactgcttct gccggaaaga tatcggctgg cgcgctatcg
     3181 aacgcgagca gaactaacgc gacagttttg ccaagagcgt tttgcgcgtg atccctaact
     3241 gacgggcggc ttcggttttg ttgccgcccg ttttctccag tgccgcaaga atcacctctt
     3301 tttcgacttc caccagcggc tgaatatcct gactttgtac cagcgggatc ggcgtactcg
     3361 caatcgccag cggcagctcg cgttcggaaa tatattcccc ggtcagcagc accaccgccc
     3421 gttccaccgc gttttccagt tcacgaatat ttcccggcca gtcgtagtga atcagcagat
     3481 ccatcgcctg gggcgtaaaa ccttttaccg ccttgcgatt acgctcggca aagcgctgaa
     3541 gaaaatggtt tgtcagcaga ggaatatctt cccgccgctg acgcagcgac ggaacttcaa
     3601 tcgccaccac gttcaggcga tagtagagat cctggcgaaa acgcccggca ttcacctctg
     3661 cggcaagatc gcgatgagtc gccgcaatca gccggacatc aaccgagatg gtctggttgc
     3721 tgccgacacg ctgaacttcg cgctcctgaa tcgcacgtag cagacgcacc tgcatcatcg
     3781 gcgagatatc gccaatttca tcgagaaaca acgtgccgcc gtccgcctca acaaagcgcc
     3841 cctcccgccg tttgtcggcc ccggtaaacg cccctttttc gtgaccgaac aattcagatt
     3901 ccagcaagga ttcgttgagt gccgcacagt tgagcgttac cagtggtttt tcactccgtg
     3961 cgctactggc gtgaatcgcc ctggcgacca gctctttacc ggtgccggaa tcgccatgga
     4021 tcagcaccgt ggcttccgat ggcgcgacga gggcgatttc actgagcagg tgttgcatcg
     4081 ccgggctttt accgaccata ccgaactggc tggcagacac cgcaggcgtt tcagcgtcaa
     4141 tactgtgcgt atgcgcgagg gccttttcca gcgtcgcctg caggttatcg aaatccagcg
     4201 gcttgatgag ataatccagc gccccggttt tcaacgcctc taccgccgtc tcgacgctgg
     4261 agtacgcggt cataatcagt accggaattg ccggatttaa cgctttgatc tctttgagcg
     4321 tggcgatgcc gtccatctct gccattcgca catcgcaaag cacaagatca aaaacctgtt
     4381 cccgcacctg ctccagcgcc tgtcgcccgc tgttcgccag cgcgacgtta tagccccagc
     4441 cgcgcagtaa agcctgcaaa atagtgcagt ggctaatgtc atcatccacc accagaatat
     4501 cgatattatc gtgcgtcatc cttgtgggtc cttacgcgta atattgaccg gaagccagag
     4561 ggtgaacgtt gcgccttttc cctcctggct tgcgacctga attgtaccac cgtgttgttc
     4621 aacaatatta tgcacgaccg ccagccccag tccggtgcct tcggctttgg tggtgaagta
     4681 cggggtgaag atggcttcaa gctgatctgc cgcaattccc ttaccgctgt cggtaacgct
     4741 gattttcaca cccgtgccgc tttcgctggc cgtcacgcta atcacgccat gctgaccaat
     4801 cgcctgaata gcattgagat agagattcaa caggacctga gtcagcctgt ccggatcggc
     4861 ctgaatttcc ggtaatgtgt cgttggcggt aaagcgtaac tggatctccc ggctgtttgc
     4921 atcctggctt accagctgta atgagtggtt aatcagcgtg ttgagatcca ccgcctgcaa
     4981 agccagatgc gttggcttaa ccagttccag caactcgctt accacgcggt ttaaacggtc
     5041 ggcttctttc gccatcacct gcgccagttg atgcgcttct ccccctgctg gcgcgcgttc
     5101 ggcaaagtat ttcgccagcc ctttaatcga ggaaagtggg ttacggattt cgtgggcaac
     5161 acctgccgca aggtgcccca gcgccaccag cttctctttg cgcttcattt catcctgcaa
     5221 caactggcgc gagcgcagat agcgacgata ccagaagaat gacaacacgc ttgccagcaa
     5281 gaccgtcgcc agggcgaaga ggataatcag ggtgtttctc tgctcacgat cttcggcact
     5341 cacaatgtta ctggcgtcga aagcaatgaa aattgcttgt ggtgtcgcgg catattgctg
     5401 catatggcgc atccggtgca ttccagcagc aaacatcggt tgaaactggc gataaatttc
     5461 cagaacaggc tcgccgtctg cgctatcgat ccgccgccac gcttcttcat ctcccggatg
     5521 taactgctgc atttcctgcg gggaataaag ctgttttccc accatgccgg agttgctatg
     5581 catcacgatt gttccttgtt catccgtgac cgcaaaccaa cgtacaccag gctgcccggc
     5641 catttcttcc agtaatgcct gctgctgcgc atgatgcatg cgcatcccca tgccgacgcg
     5701 cgagccggat tcgagagcac ggataagtac actgcctttt tccagcagcg tttgtctggc
     5761 ggcggcagtc tcgcgcccat aatcgcgaat cactgtcacc gcaaacagcc ccaccagccc
     5821 aacaatgacc acggggagga tcgcgcttaa ccatttagct aaggagtctt tagaacgttg
     5881 cataaaacgc atcttcttct ttgcctgctc atccctttct gacagcagaa atcatgccat
     5941 cttttatcag cgcttaccct gcgctgtaac acaaaggctt aagtttcaat gagtaaaaat
     6001 gactcgctac ccgcagcagg cgagtcattt ttactcgttt atcatgccag attacccgtc
     6061 atatcagcgt ttcgccgttg gcacggaaga tgcaataccc gaagtaagac aaccactgga
     6121 ggattaacca tgaaacggaa cacgaaaatt gccctggtaa tgatggcgct ttcagcaatg
     6181 gcgatgggat cgacatcggc atttgctcac ggcggacatg gtatgtggca gcaaaatgcc
     6241 gcgcctttga ccagcgaaca acagacagtc tggcaaaaaa ttcataatga cttttacgcc
     6301 caaagcagcg tgctgcaaca aaaactggtg acgaagcgtt atgaatacaa cgcgttgtta
     6361 gccgcgaacc caccggatag cagcaaaatc aatgccgttg ctaaagagat ggaaacgctg
     6421 cgtcaatcat taga
//
LOCUS       DAMZFT010000212         6307 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.212, whole genome shotgun sequence.
ACCESSION   DAMZFT010000212 DAMZFT010000000
VERSION     DAMZFT010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6307)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6307)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6307
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.212"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(<1..609)
                     /locus_tag="PN977_004819"
     CDS             complement(<1..609)
                     /locus_tag="PN977_004819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000417856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase family protein"
                     /protein_id="HDI6044747.1"
                     /translation="MELDAILDNLSDEEQIELLELLEEEENYRNTHLLYEFTPYSKQR
                     EFIDAGHDYPERCFMAGNQLGKSFTGAAEVAFHLTGRYPGTKGYPADGKYGGEWKGKR
                     FYEPVVFWIGGETNETVTKTTQRILCGRIEENDEPGYGSIPKEDIISWKKSPFFPNLV
                     DHLLVKHHTADGVEDGISICYFKPYSQGRARWQGDTIHGVWFD"
     gene            complement(587..1075)
                     /locus_tag="PN977_004820"
     CDS             complement(587..1075)
                     /locus_tag="PN977_004820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000729926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein"
                     /protein_id="HDI6044748.1"
                     /translation="MAAPKGNRFWEARSSHGRNPKFESPEALWAACCEYFEWVEANPL
                     WEMKAFSYQGEVIQEPIAKMRAMTITGLTLFIDVTLETWRTYRLREDLSEVVTRAEQV
                     IYDQKFSGAAADLLNANIIARDLGLKEQSQVEDVTPDKGDRDKRRSRIKELFNRGTGR
                     DS"
     gene            complement(1099..1497)
                     /locus_tag="PN977_004821"
     CDS             complement(1099..1497)
                     /locus_tag="PN977_004821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001595512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6044749.1"
                     /translation="MSDQQIEKEIVEKGKTAPRITPQHIEDVIKSEHYFTAYDGRNGA
                     ISSNEYCGREKPEEGDRDLSPLKLLTFCVLVLKNGFTVTGESACASPENFDAEIGRKI
                     ARQNAVNKIWMLEGYLLKQSLIESAKESSA"
     gene            complement(1786..2307)
                     /locus_tag="PN977_004822"
     CDS             complement(1786..2307)
                     /locus_tag="PN977_004822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001723433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KilA-N domain-containing protein"
                     /protein_id="HDI6044750.1"
                     /translation="MNIVPLNYKGEIVSFNTDGWINVTGVAERFGKRIDNWMRLAETL
                     EYVRALDEALTGKESQILHPSQSRYVKTSKARKDRGGGTWLHPKLSVAFARWCDARFA
                     VWCDLHIDSLLRGELTEQQKYEQACRIRDDRKSKASNGAREMARWRWDKPVIEANVEY
                     WREQLQLTLDIAC"
     gene            complement(2509..2946)
                     /locus_tag="PN977_004823"
     CDS             complement(2509..2946)
                     /locus_tag="PN977_004823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009453323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HDI6044751.1"
                     /translation="MSRVTAIISALVICIIVCLSWAVNHYRDNAIAYKDQRDKATSII
                     ADMQKRQRDVAELDARYTKELADANATIESLRDDVSAGRKRLQVAASCAKSKTGASGM
                     GDGESPRLTADAELNYYRLRSGIDKITAQVNYLQEYIRTQCLK"
     gene            complement(2943..3419)
                     /locus_tag="PN977_004824"
     CDS             complement(2943..3419)
                     /locus_tag="PN977_004824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family protein"
                     /protein_id="HDI6044752.1"
                     /translation="MVEINNQRKAFLDMLAWSEGTDNGRQKTRNHGYDVIVGGELFTD
                     YSDHPRKLVTLNPKLKSTAAGRYQLLSRWWDAYRKQLGLKDFSPKSQDAVALQQIKER
                     GALPMIDRGDIRQAIDRCSNIWASLPGAGYGQFEHKADSLIAKFKEEGGTVREIEV"
     gene            complement(3403..3726)
                     /locus_tag="PN977_004825"
     CDS             complement(3403..3726)
                     /locus_tag="PN977_004825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin, lambda family"
                     /protein_id="HDI6044753.1"
                     /translation="MKMPEKHDLLAAILAAKEQGIGAILAFAMAYLRGRYNGGAFTKT
                     VIDATMCAIIAWFIRDLLDFAGLSSNLAYITSVFIGYIGTDSIGSLIKRFAAKKAGVE
                     DGGNQ"
     gene            complement(4695..5318)
                     /locus_tag="PN977_004826"
     CDS             complement(4695..5318)
                     /locus_tag="PN977_004826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HDI6044754.1"
                     /translation="MRLESVAKFHSPKSPMMSDSPRATASDSLSGTDVMAAMGMAQSQ
                     AGFGMAAFCGKHELSQNDKQKAINYLMQFAHKVSGKYRGVAKLEGNTKAKVLQVLATF
                     AYADYCRSAATPGARCRDCHGTGRAVDIAKTEQWGRVVEKECGRCKGVGYSRMPASAA
                     YRAVTMLIPNLTQPTWSRTVKPLYDALVVQCHKEESIADNILNAVTR"
     gene            complement(5315..5503)
                     /locus_tag="PN977_004827"
     CDS             complement(5315..5503)
                     /locus_tag="PN977_004827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000994510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein ninH"
                     /protein_id="HDI6044755.1"
                     /translation="MNATIQTIPELLIQTRGNQTEVARMLSCARGTVLKYNRDSKGER
                     HVIVNGVLMVKQGKRGRP"
     gene            complement(5500..5862)
                     /locus_tag="PN977_004828"
     CDS             complement(5500..5862)
                     /locus_tag="PN977_004828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001008185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HDI6044756.1"
                     /translation="MNEYQFVLPYPPSVNTYWRRRGSQYYISDKGQKYRKDVQQIIRQ
                     LKLDIFTKSRLRIKVIADVPDSRRRDLDNILKGLLDSLIHAGFAEDDEQFDDIRVIRG
                     VKVPGGRLGIKITELENA"
     gene            complement(5859..6149)
                     /locus_tag="PN977_004829"
     CDS             complement(5859..6149)
                     /locus_tag="PN977_004829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000002235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1364 domain-containing protein"
                     /protein_id="HDI6044757.1"
                     /translation="MANLRKEARGRECQVRIYGICNGNPETTVLAHYRMAGICGTGMK
                     PGDLIGAWACSDCHAEIDRRTRILDNKDARLYHLEGVMRTQAILLKEGKIKS"
     gene            complement(6149..>6307)
                     /locus_tag="PN977_004830"
     CDS             complement(6149..>6307)
                     /locus_tag="PN977_004830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor KilAC domain-containing
                     protein"
                     /protein_id="HDI6044758.1"
                     /translation="MDRGYFTVKETAVNTNHGIQISFTTKITGRGQQWLTRKLLDNGM
                     LKVTGEAA"
BASE COUNT         1415 a         1635 c         1475 g         1782 t
ORIGIN      
        1 gtcaaaccac acgccgtgga ttgtgtcacc ctgccagcga gcgcggcctt gcgagtatgg
       61 tttgaagtag cagattgaaa tgccatcttc aacgccatcg gccgtgtgat gcttaaccag
      121 aagatgatca acaaggttcg gaaagaaagg agacttcttc cagctaatga tgtcttcttt
      181 aggtatggaa ccgtagcctg gctcatcatt ctcttcgata cgaccacaca ggatgcgttg
      241 agtcgttttg gttacagtct cgtttgtctc gccgccaatc cagaagacaa caggctcata
      301 gaaacgctta cctttccact caccgccata tttaccatca gcaggatagc cttttgtgcc
      361 cggataacgc ccggtaaggt gaaacgcgac ttcagcagca ccagtaaatg acttaccaag
      421 ctggttacca gccataaaac atcgctctgg atagtcatgc ccggcgtcga tgaactcacg
      481 ctgtttgctg tatggcgtaa attcatatag caggtgtgtg ttacggtagt tctcttcttc
      541 ttcgagtagc tcgagcaatt cgatttgctc ttcgtcgctc aggttatcaa gaatcgcgtc
      601 cagttccacg gttgaatagc tccttgatac gagagcgtcg cttatcgcga tctcccttat
      661 caggtgtcac gtcttcaact tgcgactgct ctttgaggcc caaatcacgg gcgatgatgt
      721 tagcgttgag aaggtcagca gcagcgccag agaatttctg gtcgtagatg acctgttctg
      781 ctcgcgtaac gacttcagat aaatcttctc gcaggcgata tgtgcgccat gtttcaagcg
      841 tcacatcaat gaacagagtg agtccggtaa tggtcatcgc tcgcatcttg gcgataggct
      901 cttgtatcac ttcaccctga tacgagaacg ccttcatctc ccatagcggg ttagcttcca
      961 cccactcgaa gtattcacaa caagcagccc acagcgcctc aggcgattcg aatttagggt
     1021 ttcgcccatg actactgcgg gcctcccaaa atcggttgcc ctttggtgct gccatattca
     1081 tctcacttag ttgttatttc aggctgatga ctctttcgcg ctttcaatca gtgactgctt
     1141 cagcaagtaa ccttcgagca tccagatttt gtttacagca ttctgccggg caatcttccg
     1201 accaatttct gcatcaaagt tttccgggct tgcacaggca ctctctccgg tgacggtgaa
     1261 gccattcttc agcaccagta cgcagaaagt gagcaacttc aatggtgata aatcacgatc
     1321 gccttcttct ggtttttccc tgccacaata ttcgttgctg gaaatggcac cattacgtcc
     1381 atcataagca gtaaagtaat gctcgctttt aatcacgtct tcgatgtgct gaggagtgat
     1441 tcgcggggct gttttgcctt tctcaacgat ttctttttcg atttgctggt cgctcataat
     1501 tatgaccctg tagagtggtt gcttgattag gatgtctttc catcagtccg ccaccacaaa
     1561 gaatcttttt tgccataagg caggaggttc atctttcagt ggctgccggt gttatttccc
     1621 cacttactgg cttgggttgc ttcgtggtac tgccgtaact ggttgccaag aataaattcc
     1681 ggtttcatta tcaagcccac ccgtagatgg gctttgtaat ggatagccgt tgctcagttc
     1741 tcgtaatgct ttgatttttc cgataacgca gttttgcgtt tgccatcagc acgcgatatc
     1801 gagagtcaac tgcagttgct cgcgccagta ctcaacattt gcttcaataa ccggcttatc
     1861 ccatcgccag cgagccatct ctcttgcccc attgctggct tttgatttcc ggtcatcgcg
     1921 aatgcgacat gcttgctcat atttctgctg ctcagtcagt tcaccgcgaa gcagactatc
     1981 aatgtgcagg tcgcaccaca cagcaaaacg agcatcacac caacgggcaa atgcaactga
     2041 aagttttgga tgtagccacg taccaccacc cctgtccttt cgtgccttgc tggtttttac
     2101 atacctcgat tgtgagggat gtaaaatttg agattctttc ccggtcaacg cttcgtctaa
     2161 agcacgaacg tattcaagcg tttctgccaa acgcatccag ttatcaatgc gtttcccaaa
     2221 tctctcagca acacctgtga cgttgatcca accatcagtg ttgaaactga caatttcacc
     2281 tttgtaatta agtggcacga tattcataac gtttacctac catttgaaat gaacctttgc
     2341 cgcataggaa accagcccac cgaggctcgc cagcactaac tggtatcctc aaaggcccat
     2401 tccaaagggg caggttcggt gtaaaaaaca tgcgttgcgg tacgcattta ttgcaaaaag
     2461 ccccgcatcg cgaggctcat taaattgact ttgtgatttg caaaaaaatt atttcaggca
     2521 ttgcgtcctg atgtattcct gcaggtagtt aacctgcgcg gttatcttgt cgattccact
     2581 tcggagacgg taataattga gttcagcatc tgctgtaagt cttgggcttt ctccatcgcc
     2641 catgccgctg gctccggtct ttgactttgc acaggaggcg gcgacttgca gccgcttacg
     2701 cccagcagaa acatcatcac ggagactttc gatagtcgcg ttagcatcag caagctcctt
     2761 tgtatatctg gcatcgagtt ctgctacatc acgttgacgc ttctgcatat cagcgatgat
     2821 ggatgtggct ttatcgcgct ggtctttgta ggcgatggcg ttatcacggt aatgattaac
     2881 agcccatgac aggcagacga tgatgcagat aaccagagcg gagataatcg cggtgactct
     2941 gctcatacct caatctctct gaccgttccg ccctcttctt tgaattttgc aatcaggctg
     3001 tcagccttat gctcgaactg accataacca gcccccggca gtgaagccca gatattgctg
     3061 caacggtcga tagcctgacg gatatcaccg cgatcaatca tcggtaaagc gccacgctct
     3121 ttaatctgct gcaatgccac agcgtcctgg cttttgggag agaagtcttt caggccaagc
     3181 tgcttacgat aggcatccca ccaacgggaa agaagctggt aacgtccggc tgctgttgat
     3241 ttgagtttgg ggtttagcgt gacaagtttg cgagggtgat cggagtaatc agtaaatagc
     3301 tctccgccaa caatgacgtc ataaccatga tttctggttt tctgacgtcc gttatctgtc
     3361 ccctctgacc acgccagcat atcgaggaac gccttacgtt gattattgat ttccaccatc
     3421 ttctactccg gcttttttag cagcgaagcg tttgataagc gaaccaatcg agtcagtacc
     3481 gatgtagccg atgaacacgc tcgttatata agcgagattg ctacttagtc cggcgaagtc
     3541 gagaaggtca cgaatgaacc aggcgataat ggcgcacatc gttgcgtcga ttactgtttt
     3601 tgtaaacgca ccgccattat atctgccgcg aaggtacgcc attgcaaacg caaggattgc
     3661 cccgatgcct tgttcctttg ccgcgagaat ggcggctaac aggtcatgtt tttctggcat
     3721 cttcatgtct tacccccaat aaggggattt gctctattta attaggaata aggtcgatta
     3781 ctgatagaac aaatccaggc tactgtgttt agtaatcaga tttgttcgtg accgatatgc
     3841 acgggcaaaa cggcaggagg ttgttagcgc agcctcttgc cacccgcttt cacgaaggtc
     3901 atatgtagaa ggccgcagca taactatcac tgatgaattc aggatagcca gtggctacgg
     3961 ctcagtttgg gttgtgctgt tgctgggcgg cgatgacgcc tgtacgcatt tggtgatccg
     4021 gttctgcttc cggcattcgc ttaattcagc acaacggaaa gagcatttat ggctcgcatc
     4081 gcgggaaaaa gcccacggta gagagtcgaa ctctacaaat actcttacct gttgtgcgct
     4141 ccgtttcgtg gagctaacgg cgacgctagt accggtctat gcgtcgcacc cttgagggat
     4201 ttactaaggc gacatgcccg ttgttaccca ctcaattgga ccctccccgt cgccatctgg
     4261 gtctagtcag gaatcgaacc tgccgagggt acatttgaat gggtgcccat tattaatcac
     4321 accgggccag tgcgccgaat ttggtagcgg ggagtcggaa gaccccgtga ttttggtttc
     4381 ttaggccgcc atcaacatca aatcatcgtt tgcatttatc tttgtggtca gtttctaaaa
     4441 acccgcaaag tcgccaactc tgacgaaaac tatcgttgtg ctgccacaac gataagagca
     4501 ctcggtgcat ttaagccaag ccccataagg gagaatgctc ttacctgttg tgcaaacgca
     4561 aaaagccctg agctattaac tcagggcttt atttaacgag tgcatttatc catcgttgag
     4621 tcaaatttac ccaactttat tcaataagtc aatatcatgc cgttaatatg ttgccatccg
     4681 tggcaatcat gctgctaacg tgtgaccgca ttcaaaatgt tgtctgcgat tgactcttct
     4741 ttgtggcatt gcaccaccag agcgtcatac agcggcttaa cagtgcgtga ccaggtgggt
     4801 tgggtaaggt ttgggattag catcgtcaca gctcgatatg ctgcgcttgc tggcatcctt
     4861 gaatagccaa cgcctttgca tcttccgcat tctttctcaa caactctccc ccactgctct
     4921 gtttttgcta tatcaaccgc acgacctgta ccgtggcaat ctctgcatct tgcgcccggc
     4981 gtcgcggcac tacggcaata atccgcataa gcgaatgttg cgagcacttg cagtaccttt
     5041 gccttagtat ttccttcgag ctttgccaca ccacggtatt tccccgatac cttgtgtgca
     5101 aattgcatca gatagttgat agccttttgt ttgtcgttct ggctgagttc gtgcttacca
     5161 cagaatgcag ccattccaaa tccggcttgt gattgcgcca tccccatagc agccatcaca
     5221 tcagtaccgg aaagagagtc agaagccgta gcccgtggtg agtcgctcat catcgggctt
     5281 tttggcgaat gaaatttagc tacgctttcg agtctcatgg ccttcccctt ttgccctgtt
     5341 tgaccatcag gacgccgtta actattacgt gacgctcgcc tttgctgtct cggttgtact
     5401 tgagcactgt tcctcttgcg caggaaagca tcctcgccac ttcggtctga ttgcctcgtg
     5461 tctggataag aagctctggt atcgtttgaa ttgtggcgtt catgcgttct ccagttcggt
     5521 gatttttatt ccaagccttc cacctggtac tttcacacca cgaattacgc gaatgtcatc
     5581 gaattgctcg tcgtcttccg caaatccggc gtggataagg gagtcgagta aacctttcag
     5641 gatgttgtcg aggtcgcggc ggcgggagtc tggaacgtct gcgattactt tgatgcggag
     5701 tcgtgatttg gtgaaaatgt ctaacttaag ttggcggatg atttgctgaa cgtcttttcg
     5761 gtatttctgg cctttatcgc tgatgtagta ttggcttccc cgtcttcgcc agtaggtgtt
     5821 caccgacggc gggtatggaa gcacaaactg atattcgttc atgacttaat cttcccctcc
     5881 ttcagcagta tcgcctgcgt cctcatcacg ccttcgaggt ggtaaagtct ggcgtctttg
     5941 ttgtcgagaa tccgggtgcg tcggtcgatc tccgcgtggc agtcactaca agcccatgcg
     6001 ccgatcaggt cgccaggctt cattcccgtt ccgcaaattc cagccatccg gtaatgtgcc
     6061 agaactgtag tttcaggatt gccattgcat atgccgtaaa tacgtacctg gcattctctg
     6121 ccgcgtgctt ctttgcgtag gttagccatt aagcagcctc ccctgttact ttcagcattc
     6181 cgttatcgag cagctttctg gtcagccact gttgaccacg cccggtgatt tttgtggtga
     6241 acgatatctg tattccgtga tttgtgttga ccgctgtttc tttcactgtg aaatagccgc
     6301 gatccat
//
LOCUS       DAMZFT010000213         6298 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.213, whole genome shotgun sequence.
ACCESSION   DAMZFT010000213 DAMZFT010000000
VERSION     DAMZFT010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6298)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6298)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6298
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.213"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            67..5559
                     /locus_tag="PN977_004831"
     CDS             67..5559
                     /locus_tag="PN977_004831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001313490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase"
                     /protein_id="HDI6044759.1"
                     /translation="MTFTQMANVRDKYTTSISDDMVQLVDWINSQPEKYRERLYRGAM
                     IGRMLIEYQDMKAAGHSAEQIEQQRLSLVSRLQAEIDRFGNPGRGPIAKLSGSGARAW
                     FAFRGAIKLDGTISDELTGKLVTHDSSASYDSTSYQDTLRYLYSDLTRDPIQLDDFRL
                     AFTGELPASDEELLNLLASTPGIAVSPYGGIVPFARATSGDINEIVAPKQEFLATLPD
                     GPVKNNVLNQLAAIEEKRIKTPAENIRFKLNSRWFDRSVILEFLQENGYPDLRYVQSV
                     QLEGDEMVSDTYHGGDGLFVGHRYGVVQRKDKETGEIRYEWDRKSGENATGFPAQLEK
                     YLNGARIGGKDSATANGYREQMALLEDQFNKWIKTHDRYDELVAKYNDVFNSNIPYEH
                     SGDPLGLKGLSGKRQPFDYQNSEVRRLSEDGRGILGFGTGLGKTTTALALEAFNYENG
                     RSTRTAYVVPKSVLENWYYEAKEFLSEEAFSNYLFVGLDVLMDGDQIRQVPVLDENGK
                     PVLGTDGTPVMRDALKLADEATITARMNAIPHSNYRAVVFTKEQYARIPLRDDTVDEH
                     AQDMLYDFVAAGRVASAMDSDSHRKEAARRRVLSEYSDTGTEKAEKYPYFEDMGFDSV
                     IADEGHNYRNSYKNGREASQLAYLPTSAVAQSARDMAIKNAYLMKKNGGRGPVLLTAT
                     PVVNTPIDAYNMLSHVLPKEYWQNMGIYGPDDFVKFFGKTRLETVQKISGEVEEKMAL
                     VGFENLDALRGIFHRWTTLKTAEDVKDTVEIPELDEHQQDAPLTEEQLAAYEELRQQA
                     EAAAKANNGVTTSVNEDGVIEHEKARPIFSIIRDMDRVCTDMDLYYRRITYRFLPEYA
                     DAVQQLADSLPKQATSEDDDSDDSITQQSQYSLIDKGEFIQLQVPEAFEQEVNKRLAR
                     FGIDEQTVTHPVTPKYAKLIATLKEFFPEGKQIIFTDEKTQHQKLKRIICNALNLEPS
                     KVGILNAQTVAEAGKTGKKLKAVKPPKELPDEPTDAQIAKYNEQMALYDAYIAQQNEM
                     SLGGLEKIAADFQEGRTPIIICNKKAEVGINLHRGTTDIHHLTLPWTPASIAQRNGRG
                     ARVGSNRASVRVHYYCGKGSFDEYRLKTLKRKAGWISDILRSDKSEMENADANDMIEM
                     QMYTAKDDGERLAMMQVQMDKAKAAQRARQKEQATIDLQNYIKAQHAAGEDVEVLTAE
                     LERSKAELEKTTADVAKFKQAAMAKAADNADWKARWGSVHHTDRTLLAQYRASLKSAI
                     QRKANISQAISRYEKLLNRTQKAATDIKRLRPLVEDAINKGILDVDPDLVNHANEFLV
                     IGDRSWRVGQYYDCAGDIVRIKSLDFDSQRADVEIIFTFKGTKSGNWDVKTLDKQVDV
                     TPDEDAVMQKISGGVSIAGINDIVSCDDFYRFQQRGMIKITDSYGVQTTESGYSIDFV
                     GTYTDPLKHAVYPDRRDGALKSSIAKWVLGMMSEGNNRQIRSAEAFLVELFGSNYGDV
                     IASYGDTLSPEAIQEKIADAIARMPEKTSQGATRNGDSELEVTNAIFGTNEFRASDYE
                     ITTAQFGTIGIYSNKDEIKQAMDAASARIAAERKVNLNHAVAALTQSWVTAIREAATT
                     GKITPAIADVVNDGSKFMDAYKMDAVQLPSAYGQLSYRMTYNLVSMFSDLAILGLVDL
                     NEVTPELLSMRKNHVEILHRINTVLSGRTDEEKQADADRINLALGNITEEEIAARNEK
                     QEELSSIQGDATSIAQSLGLNYRVSTADLKMMYAPKFAAGEVFGLQEASGMKGILFRA
                     KDAIKAKFGARWLPAKAKNSDFPGNWWIIETKHNVADVLAVIQQYA"
     gene            5593..6033
                     /locus_tag="PN977_004832"
     CDS             5593..6033
                     /locus_tag="PN977_004832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015974230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
                     /protein_id="HDI6044760.1"
                     /translation="MATLSDTIKPNKTYLEAVLRTALLGKTEDEYVDFFLSGLRGRLL
                     KNHRLYRSYGPYWPEIKKLLLERGYGNFGRLVDRDVRKIYRYDRPALTLIAATLYSQE
                     RFDNGQIYSAWHLLPVPEEVDDQDYEFESYDLEVEALAQAGEKT"
     gene            6030..6278
                     /locus_tag="PN977_004833"
     CDS             6030..6278
                     /locus_tag="PN977_004833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000747845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="modulator protein"
                     /protein_id="HDI6044761.1"
                     /translation="MKKRYYTVKHGTLRALQEFADKHNVEVRREGGSKALRMYRPDGK
                     WRMVVDFKTNSVPQGVRDRAFEEWEQIIIDNALLLNAD"
BASE COUNT         1702 a         1549 c         1715 g         1332 t
ORIGIN      
        1 agttatggcg cgtatagctg ggaggctctt cagcgtaacc tgactacaac agaaggccgt
       61 ctcggcatga catttaccca gatggcaaat gtccgcgata agtacaccac atcaatcagc
      121 gacgatatgg tgcaactggt ggactggatt aacagccagc ctgaaaaata ccgtgaacgc
      181 ttgtatcgcg gggcgatgat tggccggatg ttaattgaat atcaggacat gaaggccgcc
      241 gggcatagtg ctgaacaaat cgaacagcag cgcctttctc tggtatcccg tttgcaggca
      301 gagattgacc gttttggtaa ccccggtcgc ggtccgatag cgaaattgtc ggggagcggt
      361 gcgcgcgcct ggtttgcttt ccgtggtgca attaagctgg atggcactat ttctgacgag
      421 ctgacaggga agctggttac gcatgattcc agcgcaagtt atgactccac cagctatcag
      481 gacaccctgc gttatctcta tagcgatctc acccgcgatc caatccagct cgatgatttc
      541 cgccttgcgt ttactggcga actgccagcc agtgatgaag agttgcttaa tttattggcc
      601 agcacccctg gtattgcggt ttcaccgtat ggcgggattg ttccgttcgc ccgcgccacc
      661 agcggcgaca ttaacgagat agtggcacca aaacaggaat tccttgccac actccccgac
      721 ggtccagtaa agaacaacgt ccttaatcag ctggcagcga tcgaagagaa gcgcatcaag
      781 acgccagcag agaatatccg ctttaagctc aatagccgtt ggttcgaccg ttccgtcatt
      841 ctggaatttt tgcaggaaaa cggctatccg gatcttcgct atgtgcagtc agtgcagctg
      901 gaaggcgacg aaatggtttc tgacacctat cacggtggtg atggtctgtt cgtcgggcat
      961 cgatacggtg tcgtccagcg caaggataaa gaaacaggcg agatccgcta cgagtgggac
     1021 cgtaaatcag gtgaaaacgc gaccgggttc ccggcacagc tggaaaagta tctcaatggt
     1081 gcgcgtatcg gtggcaaaga tagcgcgacg gcgaacggct accgcgagca gatggcactg
     1141 cttgaggacc agttcaataa gtggatcaag acgcacgatc gctacgatga gctggttgcc
     1201 aaatacaacg atgtgttcaa tagcaatatc ccgtatgaac actctggcga tccgcttggg
     1261 ttgaagggat taagcggtaa gcgacagcca tttgattacc agaatagcga ggtgcgccga
     1321 ctgtccgaag atgggcgcgg catcctgggc ttcggtaccg ggctgggtaa aaccacgacc
     1381 gcgctggcgc ttgaggcgtt caactatgag aacggtcgtt ccacccgtac tgcgtatgta
     1441 gtgcctaaat cagtgctgga aaactggtat tacgaagcaa aagagttcct gagtgaagag
     1501 gcattcagta actacctgtt cgtcggtctt gatgtgctga tggatggcga tcagattcgc
     1561 caggtgccgg tgctcgatga gaacggtaaa cctgttcttg gtactgatgg cactccagtt
     1621 atgcgcgatg ctcttaagct ggcagatgaa gccactatca cggcgcggat gaacgcgatc
     1681 ccgcactcaa attaccgtgc agtcgtgttt accaaagaac aatacgcccg cattccgcta
     1741 cgtgatgaca ccgtagatga gcatgcacag gatatgcttt atgacttcgt tgccgccgga
     1801 cgcgtagcca gcgcaatgga ctccgactcc caccgcaaag aggccgcgcg tcgtcgggta
     1861 ttgtcggagt attcagatac cggcaccgaa aaagcagaga agtatccgta ctttgaggat
     1921 atgggcttcg atagtgtgat cgctgacgaa ggtcacaact accgcaatag ctataaaaat
     1981 ggtcgcgaag cgtcacaact ggcctatctg cccaccagcg cggtggcgca atcggcgcga
     2041 gatatggcaa ttaaaaacgc gtacctgatg aaaaagaatg gcgggcgcgg gccggttctc
     2101 ctgactgcaa cgccagtcgt taacaccccg atcgatgcat acaacatgct ttctcatgtt
     2161 ctgccgaagg aatactggca gaacatgggg atctacggtc ctgatgactt cgtaaaattc
     2221 ttcggcaaga ccaggctgga aacggtacag aaaatcagcg gtgaagttga agaaaaaatg
     2281 gcgctggtgg gctttgaaaa ccttgatgcg ctgcgcggca tattccatcg ctggacaacg
     2341 cttaaaacgg cggaagacgt taaggatacc gtggagatcc cggaactgga cgaacaccag
     2401 caggatgcac cacttactga agaacaactg gcggcgtatg aagaattgcg tcagcaggcg
     2461 gaagcggcgg ccaaagccaa caatggtgta acgacctcgg tcaatgaaga cggcgtgatt
     2521 gagcacgaga aagcccgtcc gatcttctca ataatcaggg atatggaccg cgtatgtact
     2581 gacatggacc tgtactatcg ccggatcacc tatcgtttcc tgccggagta cgccgatgcg
     2641 gtgcagcagc tggcagacag tttgcctaaa caagccacca gcgaagacga cgacagtgat
     2701 gattcaatca cgcagcaatc gcaatactcc ctgatagata agggcgagtt tattcagttg
     2761 caggttccgg aagcgttcga gcaggaagtg aataagcgcc tggccaggtt tggcattgac
     2821 gaacagaccg taactcaccc cgttacgccc aaatacgcga agctgattgc cacgctgaag
     2881 gagtttttcc cggaaggtaa gcaaatcatc ttcaccgatg aaaaaacgca gcaccagaag
     2941 ctcaagcgca ttatctgcaa tgctcttaac cttgaacctt caaaggtggg gatactgaat
     3001 gctcagacgg ttgccgaggc aggtaaaacc ggtaagaaac tgaaagcggt taaaccgccg
     3061 aaagagttac cggatgaacc aacagatgca cagatagcga aatacaacga gcaaatggct
     3121 ctgtatgacg cctatatcgc gcagcaaaat gaaatgtcgt tgggcggtct ggaaaagatt
     3181 gcagccgact tccaggaggg ccggactccg atcatcatct gcaacaaaaa ggcagaggtg
     3241 ggtatcaacc tgcatcgagg aacgactgac atccatcatc tgacgttgcc atggactcca
     3301 gccagtattg cgcaacgaaa cggtcgcggt gcccgagttg gctccaaccg tgcaagcgtt
     3361 cgcgttcact actactgcgg caagggttct ttcgatgaat accgactgaa gacgctgaag
     3421 cgtaaagcag gctggatctc cgatatcctc cgttcagata agtcagaaat ggagaacgcc
     3481 gacgccaacg atatgatcga aatgcagatg tataccgcta aggatgatgg cgaacgtctg
     3541 gcaatgatgc aggttcaaat ggataaggcg aaagccgcgc aacgcgctcg ccagaaagaa
     3601 caggctacta tcgaccttca gaactacatc aaggcgcagc acgcagctgg tgaggatgtg
     3661 gaggtgctta ccgctgaatt agagcgaagc aaagcggaac ttgaaaagac caccgccgac
     3721 gtcgccaaat tcaaacaggc ggcaatggcc aaagcagctg ataacgcaga ctggaaagcc
     3781 cgctggggta gcgtccatca cacagaccgt acgttgttag cacagtatcg cgcgtcgttg
     3841 aaaagcgcca ttcagcgcaa ggctaatatc tctcaggcca tctcccgcta tgagaaatta
     3901 ttgaaccgta ctcagaaggc cgcgacggat atcaaacgcc tgcgcccgct ggtggaggat
     3961 gcaataaata aaggcattct ggatgttgat cctgatctgg ttaaccatgc gaatgagttc
     4021 cttgttatcg gcgatcgctc atggcgtgta ggccaatact atgattgtgc cggtgatatc
     4081 gttcgcatta agtcgctgga cttcgacagc cagcgcgcag acgtggagat catcttcacc
     4141 ttcaaaggca ccaaatctgg taactgggat gtgaagacgc tggataaaca ggttgatgta
     4201 actcccgatg aagatgctgt tatgcagaaa atcagtggtg gcgtctccat cgccgggatt
     4261 aacgacatcg tttcctgtga cgatttctac cgtttccagc agcgcggcat gatcaaaatc
     4321 actgactcat acggcgttca gactacagag tcaggctata gcattgattt tgttggtacc
     4381 tatacggacc cactgaagca tgcggtttac ccggatcgcc gtgacggcgc gctgaagtcg
     4441 tcaattgcaa aatgggtgct tggtatgatg tcggaaggga ataaccgcca gatccgttcg
     4501 gcagaagcat tcctggttga actgtttggc tccaattatg gcgatgtaat cgcgtcatac
     4561 ggagatacgc tatcccctga agcaattcag gagaaaatag cggatgcgat cgccagaatg
     4621 ccggagaaaa caagccaggg ggctactcgt aacggggatt ctgaacttga agtcaccaat
     4681 gccattttcg gtaccaatga gttccgggcg tcagattatg agatcaccac agcacagttt
     4741 ggcaccattg gcatttacag caataaagac gagatcaagc aggcaatgga cgcagcaagc
     4801 gcgcgcattg cagcagaacg gaaagtcaat ctgaatcatg cagtcgccgc gctgactcaa
     4861 tcgtgggtaa cagcaatcag ggaggccgcc accacaggaa aaatcacacc ggcaattgcg
     4921 gatgtcgtaa acgacggctc taaatttatg gatgcctata aaatggatgc ggtgcagttg
     4981 ccatcagcct atgggcaact cagctatcgc atgacctaca acctggtatc aatgttttcc
     5041 gaccttgcca tccttgggct ggtggacctt aacgaggtta cgccggaatt gctcagcatg
     5101 cgcaagaacc atgtggagat attgcacaga attaacacgg tactttccgg gcgcaccgat
     5161 gaagagaaac aggcagacgc tgatcggata aacctggccc ttggcaacat cacggaggaa
     5221 gaaattgccg ccagaaacga gaaacaagaa gagttatcat caatacaggg tgatgccacc
     5281 agcatagctc agtctcttgg tctgaattat cgcgtatcca ccgccgacct gaagatgatg
     5341 tacgcaccaa aattcgccgc tggcgaggta tttgggcttc aggaggcgtc cggcatgaaa
     5401 ggcattcttt tccgtgcgaa agacgcaatc aaggcgaaat tcggcgctcg ctggctgcca
     5461 gcgaaggcga agaacagcga tttcccgggt aactggtgga ttatcgagac aaaacacaac
     5521 gtggcggacg ttctggccgt catccaacaa tacgcataac aggagcgccc ggttcgccgg
     5581 gcgtcgcata atatggccac actatctgat acaataaaac cgaataaaac atatcttgag
     5641 gcggtactcc gtacagcgtt gttaggaaag acagaagacg aatacgttga tttcttcctg
     5701 tcagggctac gcgggcgatt actgaaaaat caccgcctgt accgcagcta tggtccatac
     5761 tggccggaaa ttaaaaaatt attactggag cgcggttatg gtaatttcgg tcgtctcgtt
     5821 gaccgtgacg ttcgcaaaat ttaccgttat gaccgcccgg cgctaacact catagccgcg
     5881 acgctctaca gccaggagcg ttttgataat ggtcagatat actcagcctg gcatttactg
     5941 ccagtgcctg aagaagttga cgaccaggac tatgagtttg agtcttacga tttggaagtt
     6001 gaagccttgg cacaggctgg agagaaaact tgaaaaagcg atactacaca gtaaagcatg
     6061 ggacgctacg agcattacaa gagtttgctg acaagcataa cgttgaggtg cgcagggaag
     6121 ggggaagtaa agctctgcgc atgtaccgtc cggacgggaa atggcgtatg gtcgtcgatt
     6181 tcaaaactaa cagtgttccc cagggcgtcc gcgaccgggc attcgaagaa tgggagcaga
     6241 tcatcataga taacgcattg ctcctgaatg ctgattaaac tttccgccct tttgcccg
//
LOCUS       DAMZFT010000214         6271 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.214, whole genome shotgun sequence.
ACCESSION   DAMZFT010000214 DAMZFT010000000
VERSION     DAMZFT010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6271)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6271)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6271
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.214"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..186
                     /locus_tag="PN977_004834"
     CDS             <1..186
                     /locus_tag="PN977_004834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6044762.1"
                     /translation="HNAAAVVLAHNHPSGEVTPGKADRLITERLVQALALVDIRVPDH
                     LIIGGSRVFSFAEHGLL"
     gene            276..497
                     /locus_tag="PN977_004835"
     CDS             276..497
                     /locus_tag="PN977_004835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF987 domain-containing protein"
                     /protein_id="HDI6044763.1"
                     /translation="MKIVTRGEAMRVHRQYPSSRLFPFCTGKYRWHGGTEAYTGREVQ
                     DIPGVLAVFAERRKDSFGPYVRLMSVTLN"
     gene            514..1158
                     /locus_tag="PN977_004836"
     CDS             514..1158
                     /locus_tag="PN977_004836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000086751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin of toxin-antitoxin stability system"
                     /protein_id="HDI6044764.1"
                     /translation="MSSIITTTVYTLNELSATAREKARGWYRQHHADSRWYENVYEDF
                     RAVCEIVGIVLRQRVIRLSSGRFMEEPCIWFSGFCSQGDGACFEGRWHWQPATARRVR
                     EYAPQDRELHRITDALQAVQKHNFRQLQAEIRHRGRYCHPYSMDITVTRDSPTGQAMT
                     AGAETVVCDALRDLAFWLYSQLENEYDWLTSDDAVDEALLINGYTFTEAGLRAG"
     gene            1209..1586
                     /locus_tag="PN977_004837"
     CDS             1209..1586
                     /locus_tag="PN977_004837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system YeeU family
                     antitoxin"
                     /protein_id="HDI6044765.1"
                     /translation="MSDTLTGTTHPDDNSDRPWWGLPCTVTPCFGARLVQEGNRLHYL
                     ADRAGIRGRFSDADAYHPDQAFPLLMKQLELMLTSGELSPRHQHTVTLYAKGLTCEAD
                     TLGSCGYVYIAIYPTPATPGTTA"
     gene            1633..2007
                     /locus_tag="PN977_004838"
     CDS             1633..2007
                     /locus_tag="PN977_004838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin"
                     /protein_id="HDI6044766.1"
                     /translation="MKTFPDTHVRATSRCPSPVEIWQTLLTRLLDQHYGLTLNDTPFA
                     DERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGFSACTRSQLINSIDILRARRATG
                     LMTRSHYRMVSNITLGKHCEVK"
     gene            2004..2495
                     /locus_tag="PN977_004839"
     CDS             2004..2495
                     /locus_tag="PN977_004839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312567.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6044767.1"
                     /translation="MMKLSLTLEADSVNVRALNMGRIVADVDGVNLAELINVVCDNGC
                     SLRVVDESDRTSADCMPPFTALTGIRCSTAHITEQDNAWLYSLSHQTNDNGESEWIHF
                     TGSGYLLRTDAWSYPALRLKRLGLSKTFRRLVVTLIRRYGVSLIHLDAGAECLPGLPT
                     FDW"
     gene            2515..2712
                     /locus_tag="PN977_004840"
     CDS             2515..2712
                     /locus_tag="PN977_004840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6044768.1"
                     /translation="MKSLTTETAPDILIAWLQDNIDCGSGIIFDNDEDKTDSAALLPC
                     IEQARADIRTLRHLQLQHQNR"
     gene            2808..3656
                     /locus_tag="PN977_004841"
     CDS             2808..3656
                     /locus_tag="PN977_004841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001290190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
                     /protein_id="HDI6044769.1"
                     /translation="MSDITIPYPEAVNGHTDVICSTDIRHVVTVRNEALQQTERVIRQ
                     LAEISAMTESIGGKTARDWAVKQDFRCGCWLMEKPETAMKAITRNLDREIWRDLMQRS
                     GMLSLMDAQARDEWYRSQEDDNFPEISEANILSTSEQLHRNRDEMAERGVINLFRGLS
                     RTQNSGSPCKFGSKIILNNLVKWDWWGFGLNHGPQAGRLADLERMLYLFSGKPIPDYR
                     DNILIRLDEHIRSVQGEACYEDEMFSIRYFRKGSAHITFKHPELVEKLNAVLAKHYSV
                     KFHSSV"
     gene            3964..4623
                     /gene="glnP"
                     /locus_tag="PN977_004842"
     CDS             3964..4623
                     /gene="glnP"
                     /locus_tag="PN977_004842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015367581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter permease GlnP"
                     /protein_id="HDI6044770.1"
                     /translation="MQFDWSAIWPAIPLLIEGAKMTLWISVLGLAGGLVIGLLAGFAR
                     TFGGWIANHVALVFIEVIRGTPIVVQVMFIYFALPMAFNDLRIDPFTAAVVTIMINSG
                     AYIAEITRGAVLSIHKGFREAGLALGLSRWETIRYVILPLALRRMLPPLGNQWIISIK
                     DTSLFIVIGVAELTRQGQEIIAGNFRALEIWSAVAVFYLIITLVLSFILRRLERRMKI
                     L"
     gene            4620..5342
                     /gene="glnQ"
                     /locus_tag="PN977_004843"
     CDS             4620..5342
                     /gene="glnQ"
                     /locus_tag="PN977_004843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000569103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter ATP-binding protein
                     GlnQ"
                     /protein_id="HDI6044771.1"
                     /translation="MIEFKNVSKHFGPTQVLHNIDLNIAQGEVVVIIGPSGSGKSTLL
                     RCINKLEEITSGDLIVDGLKVNDPKVDERLIRQEAGMVFQQFYLFPHLTALENVMFGP
                     LRVRGANKEEAEKLARELLAKVGLAERAHHYPSELSGGQQQRVAIARALAVKPKMMLF
                     DEPTSALDPELRHEVLKVMQDLAEEGMTMVIVTHEIGFAEKVASRLIFIDKGRIAEDG
                     DPQVLIKNPPSQRLQEFLQHVS"
     gene            5459..>6271
                     /locus_tag="PN977_004844"
     CDS             5459..>6271
                     /locus_tag="PN977_004844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415329.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel protein"
                     /protein_id="HDI6044772.1"
                     /translation="MRWILFILFCLLGAPAHAVSIPGVTTTTTTDSTTEPAPEPDIEQ
                     KKAAYGALADVLDNDTSRKELIDQLRTVAATPPAEPVPKIVPPTLVEEQTVLQKVTEV
                     SRHYGEALSARFGQLYRNITGSPHKPFNPQTFSNALTHFSMLAVLVFVFYWLIRLCAL
                     PLYRKMGQWARQKNRERSNWLQLPAMIIGAFIIDLLLLALTLFVGQVLSDNLNAGSRT
                     IAFQQSLFLNAFALIEFFKAVLRLIFCPNVAELRPFTIHDETARYWSRRLSWL"
BASE COUNT         1453 a         1721 c         1661 g         1436 t
ORIGIN      
        1 cacaatgccg ctgccgtggt gctggcacac aatcatccgt ccggtgaagt cacacccggt
       61 aaggcagacc ggcttatcac tgaacgtctg gtacaggcac tggccctggt ggatatccgg
      121 gtgccggacc atctgataat cggtggcagc cgggtgttct cctttgccga acacggtctg
      181 ctttaatccg tcaccgtcac aaccaccttc atattgtcac ttcagtttct ctttctcagc
      241 tgtttctcac tttcacattc aggaggactg ttctcatgaa aatcgtcacc cgtggtgaag
      301 ccatgcgcgt tcaccgtcag tatccgtcat cccgtctttt tccgttctgc accggtaagt
      361 accgctggca cggcggcact gaggcgtata ccggtcgtga agtgcaggat atccccggcg
      421 tactggccgt gtttgccgaa cgccgcaagg acagttttgg cccgtatgtc cggctgatga
      481 gcgtcaccct gaactgagtc agtggagatg ctgatgagca gcattatcac caccaccgtg
      541 tatacgctga atgaactttc cgccacagca cgggaaaaag cccgcggctg gtaccgtcag
      601 catcatgctg acagccgctg gtatgaaaac gtgtatgagg acttcagggc ggtctgtgag
      661 attgtcggga ttgtcctccg ccagcgtgtt atccggctca gcagcggcag gtttatggaa
      721 gagccgtgca tctggttcag cggattctgc agccaggggg acggagcctg ttttgaaggg
      781 cgctggcact ggcagccggc aacagccagg cgagtccggg aatatgcccc gcaggaccgt
      841 gagcttcatc gtatcacgga tgccctgcag gcggtgcaga agcataactt ccggcagtta
      901 caggctgaaa tccgtcaccg gggacgctac tgccatccgt acagcatgga tatcaccgtc
      961 acccgggaca gcccgaccgg acaggcaatg acagccggcg cggaaaccgt tgtctgtgac
     1021 gccctgcggg acctggcctt ctggttatac agtcaactgg aaaacgagta cgactggctg
     1081 acctcagatg acgccgtgga tgaggcgctt ctcatcaacg gatacacctt caccgaagcc
     1141 ggtctgcgcg ccggctgacc cgtcattgtt aaaaccattc ttaaccgatt atcagtgagg
     1201 actttatcat gtcagacaca ctcaccggga caacgcatcc cgacgacaac agcgaccgcc
     1261 cctggtgggg gctgccctgc accgtgacgc cctgtttcgg tgcccgtctg gtgcaggagg
     1321 gtaaccgcct gcattacctt gcagaccgcg ccggcatcag aggccggttc agcgacgcag
     1381 atgcgtacca cccggaccag gcctttccgc tgctgatgaa acagctggaa ctcatgctca
     1441 ccagtggtga actcagtccc cgccatcagc ataccgtcac actgtatgcg aaagggctga
     1501 cctgcgaagc cgacaccctc ggcagttgtg gttacgtgta tatcgccatt tatccgacac
     1561 cggcaacacc cggaaccacc gcataacccc ggacagcact tctttatcac cacctgttac
     1621 agagtactta agatgaaaac attccccgac acacatgtac gggcaacatc gcgctgcccg
     1681 tctccggttg aaatctggca gacactgctc acccggttgc tggaccagca ctacggcctc
     1741 acgctgaatg acacaccgtt cgccgatgaa cgtgtgattg aacagcatat tgaggcaggt
     1801 atttcactgt gtgatgcggt gaactttctc gtggaaaaat acgcactggt gcgtaccgac
     1861 cagccgggat tcagtgcctg cacccgctct cagttaatca acagcattga tatcctccgg
     1921 gcccgccggg caaccggcct gatgacccgc agccactacc gaatggtgag caacatcacc
     1981 ctgggtaaac actgtgaggt gaaatgatga aactgtccct gacgctggaa gcagacagcg
     2041 ttaacgtccg ggcactgaac atggggcgca ttgtcgctga cgtcgatggt gttaatctcg
     2101 ctgaactgat taacgtggtc tgcgataacg gctgttccct tcgtgttgtt gatgaatctg
     2161 accggacctc agcagactgc atgccaccat ttactgccct taccggcata cgctgcagta
     2221 ccgcacatat cacggaacag gacaatgcct ggctgtactc gctgtcgcat cagacgaatg
     2281 acaacggcga atcagaatgg attcatttta caggcagcgg atatctgtta cgtactgatg
     2341 cgtggtcgta cccggctctg cggcttaaac gcctggggct gtcaaaaacc ttccgccgtc
     2401 tggttgtcac acttatccgg cgttatggcg tcagtctcat tcatctggat gccggcgctg
     2461 aatgtctgcc gggtttaccc actttcgact ggtaaccggg acaaaagaca ccgtatgaaa
     2521 tcattaacca cagaaacggc accggatatt ctgattgcgt ggctgcagga caatatcgac
     2581 tgcggctccg gcattatctt cgacaacgat gaggataaaa cggattcggc agcactgctg
     2641 ccctgtatcg aacaggccag agcggatatc cgcaccctgc gccacctgca gcttcagcac
     2701 cagaaccggt gagtctcact catcatctca ctcaccacga ttcattcccc tgacgccagt
     2761 ccgaatacgg ctggcgtttt catttataaa ccacaaggaa aaatgtaatg tctgatatca
     2821 caatccctta tccggaagcg gtcaacggac atacggacgt tatctgctcc acagatatcc
     2881 ggcacgttgt gacggtacgg aatgaagcac tgcaacaaac cgaaagggtt atccggcaac
     2941 tggcagaaat ctcagcaatg acagaaagca taggcggtaa aacggcgcgg gactgggccg
     3001 tgaaacagga ttttcgctgt ggctgctggc tgatggaaaa accggaaacc gcaatgaaag
     3061 ccatcacccg gaaccttgac cgtgaaatct ggcgtgacct gatgcaacgc tccgggatgc
     3121 tgagtctgat ggatgcacag gcgcgtgatg aatggtaccg ttcacaggag gacgataatt
     3181 tcccggaaat cagcgaagcg aatatcctga gcacgtctga acaactgcac cgtaacaggg
     3241 atgaaatggc tgaacggggc gtcatcaacc tcttcagggg actgagccgg acgcaaaaca
     3301 gcgggtcacc ctgtaaattc ggcagcaaga ttatcctcaa caacctggtg aaatgggact
     3361 ggtggggatt tggtctgaac catggcccgc aggccgggcg gcttgccgac ctggaaagaa
     3421 tgttgtatct gttcagtggt aaacccatac ccgattacag ggacaatatc ctcattcgtc
     3481 tggatgagca catccggtcc gttcagggtg aagcgtgtta tgaagatgag atgttcagca
     3541 tcagatactt caggaaaggc tctgcgcata tcacgtttaa acacccggaa ctggtggaga
     3601 agctgaatgc tgttctggcg aaacattatt cggtaaagtt ccattcttct gtataaactt
     3661 acagatcacg ggtattgtgt ctcgtcctga acagggccga caggtatact gcattcgctg
     3721 agccattaca gataatgcct caactgagtt caatctacct ccatgtacaa caacaatacg
     3781 atgggggcta cattgggggc tatactttca agtaaaactg atataaccaa gattaaacaa
     3841 taagatagag aattttcacc attcccctct ggggagagga ttagggtgag gggcgcaaac
     3901 ccgctccggg gccattaatt accctgaatt tgattattta caccacggta acaggaacaa
     3961 catatgcagt ttgactggag tgccatctgg ccagccattc cgcttctgat tgaaggtgcc
     4021 aaaatgaccc tgtggatctc ggtcctcggt ctggctggcg gcctggtgat cggattgctg
     4081 gcaggttttg cacgcacctt cggtggttgg atagccaacc acgtcgcgct ggtctttatt
     4141 gaagtgatcc gcggcacacc tatcgtcgtc caggtgatgt ttatctattt cgccctgccg
     4201 atggcgttta acgacttacg catcgacccg tttactgcgg cggtggtcac catcatgatc
     4261 aactctggcg cgtacattgc ggaaatcacg cgtggtgcgg tgctgtctat tcacaaaggt
     4321 tttcgtgaag caggactggc gctcggtctt tcacgttggg aaaccattcg ctacgtcatt
     4381 ttaccgctgg cactgcgtcg tatgctgccg ccgctgggta accagtggat catcagcatt
     4441 aaagacacct cgctgtttat tgttatcggt gtggcggaac tgacccgtca ggggcaagaa
     4501 attattgccg gtaacttccg cgcccttgag atctggagcg ccgtggcggt gttctatctg
     4561 attattaccc tggtgctgag ctttattctg cgtcgtctgg aaagaaggat gaaaatcctg
     4621 tgattgaatt taaaaacgtc tccaagcatt ttggtccaac ccaggtgctg cacaatatcg
     4681 atttgaacat tgctcagggc gaagtcgtgg tgattatcgg gccgtccggt tccggtaaat
     4741 cgaccctgct gcgctgcatc aacaaactgg aagaaatcac ttccggcgat ctgattgtcg
     4801 atggcctgaa ggttaacgat ccgaaagttg acgagcgcct gattcgccag gaagcaggta
     4861 tggtgttcca gcagttttac ctcttcccgc atctgacggc gctggaaaac gtcatgtttg
     4921 gtccgctacg cgtgcgtggc gcgaacaaag aagaggcgga aaaactggcg cgtgagctgc
     4981 tggcgaaagt cggtctggca gaacgtgcac atcactaccc ttccgaactt tctggtggtc
     5041 aacagcagcg tgtggcgatc gcacgcgcgc tggcggtgaa gccgaaaatg atgctgtttg
     5101 atgaaccgac ttccgctctt gacccggaac tgcgccatga agtgctgaag gttatgcagg
     5161 atctggctga agaagggatg acgatggtga tcgtgacaca cgaaatcggt tttgccgaga
     5221 aagtagcttc gcgcctgatc tttatcgaca aaggccggat tgcggaagat ggcgatccgc
     5281 aggtgttgat caagaaccca ccgagccagc gcttgcagga atttttgcag cacgtctctt
     5341 aacttctcag cctccctttc ccccgaaagg gaggcacacc agattcctct catttaaaat
     5401 cgcccctcct ccagcatcta tacttatctt tttgctctgt tttctcactg gaggagtcat
     5461 gcggtggatc ctgttcatcc tcttctgcct gctgggcgca cctgcccacg cggtatccat
     5521 acccggcgtt acaaccacaa cgacaacgga ctcaacgact gaaccggccc cggaaccgga
     5581 tatcgaacaa aaaaaagcgg cctatggcgc actggcggat gtgctggata atgacacctc
     5641 gcgtaaagag ttgatcgacc agttgcgcac cgtcgccgcg acgcccccgg cagaacccgt
     5701 accgaagatc gtgccgccga cgctggttga agagcaaacc gtgctgcaaa aggtcaccga
     5761 agtcagccgc cattatggtg aagccctttc cgcccgcttc gggcaacttt atcgcaatat
     5821 caccggctcc ccgcataagc cgtttaatcc acaaaccttc agcaatgcgt tgacccattt
     5881 ttcaatgtta gcggtattag tgtttgtttt ttactggctg attcgcctgt gcgcacttcc
     5941 gctgtatcgc aaaatgggcc agtgggcgcg gcaaaaaaat cgtgagcgca gtaactggtt
     6001 gcagcttccg gcgatgatta tcggggcgtt tattatcgac ctgctgttac tggcactgac
     6061 attgtttgtc ggccaggtat taagcgacaa cctgaatgcg ggcagtcgca ccatcgcttt
     6121 ccaacaaagt ttgtttctca acgcctttgc cctgattgaa tttttcaaag ccgtactacg
     6181 cctgattttt tgcccaaacg tggctgaact gcgcccgttc acgattcatg acgagaccgc
     6241 ccgttactgg agccgtcgcc tgagctggtt a
//
LOCUS       DAMZFT010000215         6264 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.215, whole genome shotgun sequence.
ACCESSION   DAMZFT010000215 DAMZFT010000000
VERSION     DAMZFT010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6264)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6264)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6264
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.215"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(102..400)
                     /locus_tag="PN977_004845"
                     /pseudo
     CDS             complement(102..400)
                     /locus_tag="PN977_004845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000867283.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="rod-binding protein"
     gene            complement(400..1500)
                     /locus_tag="PN977_004846"
     CDS             complement(400..1500)
                     /locus_tag="PN977_004846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001181884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HDI6044773.1"
                     /translation="MQNWIKAAVITVSLALPGVALAQSLESLVNVQGVRENQLVGYSL
                     VVGLDGTGDKNQVKFTNQTITNMLRQFGVQLPNKIDPKVKNVAAVAVSATLPPMYSRG
                     QTIDVTVSSIGDAKSIRGGTLLLTQLHGADGEVYALAQGSVVVGGMNATGASGSSVTV
                     NTPTAGLIPNGATVEREIPSDFQMGDTITLNLKRPSFKDANNIAAAINASFGGIATAQ
                     SSTNVSVRAPTSPGARVAFMSQLDDVQVQAEKIRARVVFNSRTGTVVMGDGVALHAAA
                     VSHGSLTVSINETSNVSQPNAFAGGRTAVTPQSNIAVNHARPGVVSLPESSSLKTLVN
                     ALNSLGATPDDIMSILQALHEAGALDADLEVI"
     gene            complement(1515..2180)
                     /gene="flgH"
                     /locus_tag="PN977_004847"
     CDS             complement(1515..2180)
                     /gene="flgH"
                     /locus_tag="PN977_004847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002211429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HDI6044774.1"
                     /translation="MKNYLWLVALLPLLSGCESQAILVKKDDAYFAPPTTEAPPPADG
                     RAGGVFETGYNWSLTADRRAYRVGDILTVILEESTQSSKQAKTNFGKSNTVDIGAPTI
                     FGHTKDKLSGSIDANRDFDGSATSQQQNSLRGEITVSVHAVQPNGILEIRGEKWLTLN
                     QGDEYIRLSGLVRADDIQNDNSVSSQRIADARISYAGRGALSDANAAGWLTRLFNHPL
                     FPI"
     gene            complement(2263..3048)
                     /gene="flgG"
                     /locus_tag="PN977_004848"
     CDS             complement(2263..3048)
                     /gene="flgG"
                     /locus_tag="PN977_004848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003838810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
                     /protein_id="HDI6044775.1"
                     /translation="MNAALWISKTGLSAQDAEMSAIANNIANVNTTGFKRDRVMFQDL
                     FYQTQEAPGAMLDQNNIMPTGLQFGSGVRIVGTQKTFTEGNVETTDNAMNVAIMGQGF
                     LQVQKANGDIAYTRDGNLQVNADGVLTNSQGLPLQPEIDVPAGATNVAFGEDGTVTAI
                     LPGDSDVTELGQLTLVNFANPAGLSAEGDNLYLETAASGQPTEGVPGEDGLGTLQDNA
                     LEGSNVDIVNEMVAMITVQRAYEMNAKMVSAADDMLQYISQTL"
     gene            complement(3127..3864)
                     /locus_tag="PN977_004849"
     CDS             complement(3127..3864)
                     /locus_tag="PN977_004849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000375458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgF"
                     /protein_id="HDI6044776.1"
                     /translation="MDRLIYTALSGASQTLYEQQISANNLANVNTNGFRADMAMATND
                     KVKGGGFDTRYMAQEGASGVNDSTGVAEKTERPLDVAIQGAGYIAVQDKNGNEVYTRN
                     GNIQQDDQGQLTIDGNLVLGDNGPIILPPNAIASFGSDGTLSVTPDDGDVTATMDIDR
                     LKLVDIPVANLAKNPEGMLVTSDGIPAQRDENIKVSGGFLEGSNVSAVSEMMSSIAMN
                     RQFEAQIKMMKTAEDISDAGNRLLRGS"
     gene            complement(3864..5066)
                     /gene="flgE"
                     /locus_tag="PN977_004850"
     CDS             complement(3864..5066)
                     /gene="flgE"
                     /locus_tag="PN977_004850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body protein FlgE"
                     /protein_id="HDI6044777.1"
                     /translation="MSYEIAATGLNAVNEQLDGISNNIANAGTVGYKSMTTQFSAMYA
                     GSQAMGVSVAGTAQSISRGGSLVSTGNALDLAINDDGFFVTCDSAGNISYTRAGSFET
                     DKNGYIVNASGAYLQGYPVDDTGTLQTGTVTDIQIKTGNIPAQASSSLTFTANFDASD
                     DAIDRTTVPFDATNSSSYTDSYTTTVYDSLGNEHSVCQYFTKTSDNTWEVQYTFDGQQ
                     QTGVPATTLTFDPNTGKLTSPTTPQTIEFQTDAAAPIDLTVDYSTCTQYGSEFSVTTN
                     AANGYASATQNGVQVDDDGKVYATYSNGERMLQGQVVLATFPNENGLEAVSGTAWVQT
                     GESGTPLIGVPGSGSCGTLSSGVLESSNVDITSELVNLMTAQRNYQANTKVIATSTQL
                     DDALFQAM"
     gene            complement(5089..5802)
                     /locus_tag="PN977_004851"
     CDS             complement(5089..5802)
                     /locus_tag="PN977_004851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001508388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook capping protein"
                     /protein_id="HDI6044778.1"
                     /translation="MNTLALNAQSPQPTATPESNNVAASDTTSSSDSSPVDTFLTLFV
                     AEIQNQDPTDPTDATEYIDQLSSMAQVAMMEEMSVQANTNAVLMSNIQVMALGNLVGD
                     DIMVQTTALQVSDQTINGRATLDDACTTADLHFTDAAGDDYTVSLIPEGSSSVGPGQV
                     DFSVNPADYGIPPGDYDVSVVTNTGEEEVPIEVSGEVEDVRIPLDGSSPVLNVAGVGE
                     VPFTMISQFGVPDADSDVA"
     gene            complement(5802..6233)
                     /gene="flgC"
                     /locus_tag="PN977_004852"
     CDS             complement(5802..6233)
                     /gene="flgC"
                     /locus_tag="PN977_004852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000010944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HDI6044779.1"
                     /translation="MSFTDIYQISGSAMTAQTLRLNTVASNLANANAPASSEAQAYKA
                     RSPVFAAVYHHSLLAGTHRHAIDGASVQVQDVLQTGGAVKRYEPHSPLADANGDVWYP
                     DVNVVEQMADMMSASRDFETNVDVLNNVKSMQQSLLKLGEA"
BASE COUNT         1168 a         1865 c         1758 g         1473 t
ORIGIN      
        1 ccttatccgg cctacaaacg gacacacgca taaaaacagg tgcggactcg taggcctgat
       61 aagacgagac agcgtcgcat caggcaccga acgtcggagc ctcatttatg cttcggcgac
      121 aactgcttca caatcatcgc cgcaatccca atcccgtgct gctgcgccag ggtgttacac
      181 aactcatcgt cataaaaccc ctgcatcatg cgcaccgaat cgctgttaaa cgggttatct
      241 ttcgaatcaa acaactcgtt ggttttacgc atctctttca gcatgttgcg taaaaaatcg
      301 cctcaaactg ctccgcggcc tgcttaatat cattggcctg aaccttcggc cccattaacg
      361 catcgctgcc atcaatcccg ccgctggcgt tcactttcat cagatcacct ccaggtcggc
      421 atccagcgcg cccgcctcat gcagcgcctg taaaatcgac ataatgtcgt ccggcgtcgc
      481 gcccaaactg ttcaacgcat tgaccagcgt tttcaggctg ctcgactccg gcaaactcac
      541 cacgcccggg cgcgcatgat tcaccgcgat attgctctgc ggcgttaccg ccgtgcgccc
      601 gcctgcaaag gcattcggct ggctgacgtt gctggtttcg ttaatcgaga cggtcaaact
      661 gccgtgcgac accgccgccg catgcagcgc aacgccatcg cccatcacca ccgtgccggt
      721 acgcgagtta aacaccacgc gggcgcggat tttctctgcc tgcacctgta catcatccag
      781 ttgcgacata aatgccaccc gcgcgccggg gctggtgggc gcgcgcacgc tgacgttggt
      841 ggagctctgc gcagtagcaa tgccgccaaa cgaggcatta atcgccgccg caatattgtt
      901 ggcatcttta aacgacggac gtttcaggtt gagggtgata gtgtcgccca tctgaaaatc
      961 gctggggatc tcgcgctcta ccgttgcgcc gttaggaatt aacccggcgg ttggcgtatt
     1021 aaccgttacg ctcgaaccgc tggccccggt ggcattcatc ccgcccacca ccacgctgcc
     1081 ctgcgccagg gcatacactt cgccatccgc gccgtgcaac tgggtcagca acaaagtgcc
     1141 gccacgaata cttttggcat cgccaatcga cgaaacggtg acatcaatgg tctgcccgcg
     1201 cgagtacatc ggcggcagcg tggcgctcac cgccaccgcg gcgacgtttt tcaccttcgg
     1261 gtcgatttta ttcggcagtt gcacgccaaa ctggcgcagc atattggtga tggtctggtt
     1321 ggtgaatttg acctggtttt tatcgcccgt gccgtcgagg ccgaccacca ggctgtagcc
     1381 gaccagttgg ttttcacgca cgccctgtac gttgaccagc gattccagcg attgcgccag
     1441 cgccacgcca ggtagcgcca ggctcacggt gattactgcg gcctttatcc agttttgcat
     1501 tgcactctcc ggcattaaat cgggaacaga ggatggttaa atagccgcgt caaccagcct
     1561 gcggcgttgg cgtcgcttaa cgcgccgcgt ccggcatagg agatgcgggc atcggcgatc
     1621 cgctgggagg aaaccgagtt gtcgttctgg atgtcgtcgg cgcgcaccag gccgcttaag
     1681 cggatgtact catcgccctg gttgagggtc agccattttt cgccgcgaat ttccaggatg
     1741 ccgttaggct gcaccgcatg gacggaaacg gtgatctccc cgcgcaggct gttctgctgc
     1801 tgggaagtgg cactgccgtc aaagtcgcgg ttggcgtcga tagagccgga aagcttatct
     1861 ttggtgtgtc caaaaatcgt tggcgcgccg atatcgacgg tgttgctttt gccgaagttg
     1921 gttttcgcct gtttgctcga ctgggtggat tcttcgagga tcaccgtcag gatatcgccc
     1981 acgcggtaag cccgccggtc ggcagttagc gaccagttgt agccggtttc aaacacgccc
     2041 ccggcacgcc cgtctgccgg aggcggcgct tcggtggttg gtggcgcaaa gtatgcgtcg
     2101 tcttttttga ccagaatggc ctgcgattca cacccgctaa gcaggggaag cagcgccacc
     2161 agccagagat aatttttcac gttctgcctg tttaaataca gtgagttatg tgctcttgta
     2221 ggtcggataa gacgtgtcag cgtcgcatcc gacatttcgg ccttacagcg tctgactgat
     2281 gtactgcaac atatcgtccg cagccgacac cattttggcg ttcatctcgt acgcccgttg
     2341 caccgtaatc atcgccacca tctcgttgac gatatccacg ttcgagcctt cgagcgcgtt
     2401 gtcctgcaag gtgccaagcc catcttcgcc cggtacgcct tcggtcggct gaccgctggc
     2461 agcggtttcc agatacaggt tgtcgccctc ggcggaaagc cccgccgggt tagcgaagtt
     2521 caccagtgtc agttgcccta actcggtcac atcgctgtcg ccgggtaaga tcgccgtcac
     2581 cgtgccgtcc tcaccaaacg caacgttagt cgcccccgcc ggaacgtcga tttccggttg
     2641 taacggcaaa ccctgcgagt tagtcaacac gccgtcggcg ttcacctgca agttgccatc
     2701 gcgggtatag gcgatatcgc cgttagcctt ttgcacctgc aaaaatccct gtcccataat
     2761 ggcgacattc atggcgttgt cggtggtctc aacgttgcct tcggtgaagg ttttctgggt
     2821 accgacaatg cgtacgccgc tcccgaattg cagcccggtc ggcatgatgt tgttctggtc
     2881 gagcatcgcg cccggcgctt cctgggtctg gtagaacaga tcctgaaaca tcacccgatc
     2941 gcgcttgaag ccggtggtgt taacgttggc gatgttgttg gcaatcgcgc tcatttcagc
     3001 atcctgcgcc gagaggccgg ttttgctgat ccatagtgct gcgttcatgg tggtaaattc
     3061 cgtgttattt ttgggccgca gttgccggat gcggcgtgaa cgccttatcc ggcctacgat
     3121 gtgcggttac gagccgcgca ataagcggtt gcccgcgtcg ctgatatctt cggctgtttt
     3181 catcatcttg atctgcgctt caaactggcg gttcatggcg atggacgaca tcatttcgct
     3241 caccgccgag acgttgctgc cttcgagaaa accgccgctg actttgatgt tttcatcgcg
     3301 ctgggccgga atgccgtcgc tagtcaccag catcccttca ggatttttcg ccaggttagc
     3361 gaccgggata tccaccagct tcaggcggtc gatatccatc gtggcggtca cgtcgccgtc
     3421 atcaggcgtc accgacagcg tgccgtcgct gccaaacgag gcaatggcgt tcggcggcag
     3481 aatgatcggg ccgttatccc ccagcaccag attgccgtcg atggtcagtt gcccctgatc
     3541 gtcctgctga atgttaccgt tgcgggtata gacttcgttg ccgtttttat cctgcaccgc
     3601 aatgtaaccc gcgccctgaa tcgccacgtc cagcgggcgt tcggttttct ccgccacacc
     3661 cgtgctgtcg ttcacgccgc tggccccttc ctgcgccata tagcgagtgt cgaagccgcc
     3721 gcctttcacc ttgtcattgg tcgccattgc catatcggcg cgaaaaccgt tggtgttgac
     3781 gttcgccagg ttgttggcgc taatctgctg ctcatacaac gtctgagaag ccccgctcag
     3841 ggcggtataa atcaagcgat ccattacatt gcctggaaca gcgcgtcatc gagctgcgtg
     3901 ctggtggcga tcactttggt gttggcctga tagttacgct gggcggtcat caggttgact
     3961 aactcgctgg tgatatcgac gttagagctt tcgagcacgc ccgacgacag cgtgccgcag
     4021 gaaccggagc cgggaacgcc aatcagcggc gtgccggatt ccccagtttg tacccacgcg
     4081 gtgccgctca ccgcttcgag tccgttttca ttcgggaaag tcgccagcac cacctggccc
     4141 tgcaacatgc gctcgccgtt gctgtaggta gcgtaaactt tgccatcgtc atcgacctgc
     4201 acaccgtttt gcgtagcgga ggcgtagccg ttggcggcgt tggtggtgac cgaaaattca
     4261 gagccgtatt gcgtacaggt ggagtaatcg acggttaagt cgatgggtgc ggcggcgtcg
     4321 gtctgaaact caatggtctg cggcgtggtt ggcgaagtca gcttcccggt gttcgggtcg
     4381 aaggttaacg tcgtcgccgg aacgccggtc tgctgctgac cgtcgaaggt gtactgcact
     4441 tcccaggtgt tgtcgctggt tttggtgaag tactggcata ccgagtgttc gttacctaat
     4501 gagtcgtaca ccgtggtggt gtagctgtcg gtatacgagc tgctgttggt cgcgtcgaac
     4561 ggtacggtag tgcggtcgat agcgtcgtcg ctggcatcga agttggcggt aaaggtcagg
     4621 ctgctgctgg cctgcgccgg aatattgcca gttttgatct ggatatcggt gactgtaccg
     4681 gtttgcagag tgcccgtgtc atcgaccgga tagccctgca aataagcgcc tgaggcgttg
     4741 acgatatagc cgtttttgtc ggtttcaaac gatccggcgc gggtataaga aatgttgccc
     4801 gcgctgtcgc aggtaacaaa aaaaccgtcg tcgttaatcg ccagatccag cgcgttaccg
     4861 gtggatacca gcgaaccgcc gcgcgaaata ctttgcgcgg tgcctgccac gctgacgccc
     4921 atcgcctgac ttccggcata catggctgaa aactgggtgg tcatcgactt atagcctacc
     4981 gttccggcgt tggcgatgtt gttactgatc ccgtccagct gttcgttaac ggcattcagc
     5041 cccgttgcgg caatttcata actcattgtt tcttcctgtt aattgttctt acgccacatc
     5101 actgtcggca tcgggtacgc caaactggct aatcatggtg aacggcactt cacctacacc
     5161 ggcaacgtta agtaccgggg agctgccgtc gagcgggatg cgcacatctt ccacttcgcc
     5221 ggagacttca atcggcacct cttcttcccc ggtgttggtc accaccgaga cgtcgtaatc
     5281 gcccggcggg ataccgtaat ccgcgggatt caccgagaaa tccacctggc ctggcccgac
     5341 ggacgaactc ccttccggga tcagcgacac cgtatagtca tcgcctgcgg catcggtaaa
     5401 gtggagatcg gcggtggtac aggcgtcgtc aagcgtggcg cgaccgttaa tggtctggtc
     5461 actcacctgc aaggcggtgg tctgcaccat aatgtcgtcg cccaccaggt tgccgagcgc
     5521 catcacctga atgttgctca tcaacaccgc gttggtattc gcctgcacgc tcatctcttc
     5581 catcatcgcc acctgcgcca ttgacgaaag ctggtcgata tattcggtgg cgtcagtcgg
     5641 gtcggtcggg tcctggtttt ggatctcagc gacaaacagc gtcaggaagg tatcgacggg
     5701 cgagctatcg ctgctgcttg tcgtgtcgct ggccgccaca ttattgcttt ccggcgtggc
     5761 ggtcggctgc ggtgattgcg cattcagcgc cagggtattc atcaggcttc tcccagtttc
     5821 agcaggcttt gctgcatact tttcacgttg ttgagcacat cgacgttagt ttcaaaatcg
     5881 cgcgaggccg acatcatgtc cgccatctgt tccaccacgt tgacgtcggg ataccagaca
     5941 tccccgttag catcggcgag cggcgaatgt ggttcatagc gtttcaccgc cccgccggtt
     6001 tgcagcacgt cctgcacctg cacactggca ccgtcaatgg catgacgatg tgtacccgcc
     6061 agcaaactgt ggtgatacac cgccgcaaac accgggctgc gcgctttata tgcctgcgcc
     6121 tcgctgctgg cgggcgcatt agcattcgcc aggttactgg cgacggtatt cagacgtagc
     6181 gtttgcgccg tcatcgccga gccggaaatc tgatagatat cagtaaatga catgcttatt
     6241 tcccttcaat ggcctctctg atgc
//
LOCUS       DAMZFT010000216         6230 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.216, whole genome shotgun sequence.
ACCESSION   DAMZFT010000216 DAMZFT010000000
VERSION     DAMZFT010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6230)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6230)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6230
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.216"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(<1..245)
                     /gene="flgB"
                     /locus_tag="PN977_004853"
     CDS             complement(<1..245)
                     /gene="flgB"
                     /locus_tag="PN977_004853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002211435.1"
                     /note="with FlgF and C makes up the proximal portion of
                     the flagellar basal body rod; Yersinia has 2 copies of
                     flgB and other flagellar genes; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HDI6044780.1"
                     /translation="MGISFQQALGVHPQAVKLRLERTELLTANLANVDTPNFKAKDID
                     FAREMQRANNAAVDVQYRVPMQPSEDGNTVELNSEQAR"
     gene            327..1064
                     /gene="flgA"
                     /locus_tag="PN977_004854"
     CDS             327..1064
                     /gene="flgA"
                     /locus_tag="PN977_004854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring formation protein
                     FlgA"
                     /protein_id="HDI6044781.1"
                     /translation="MSFQFCRRKFYFLLALALPGYAAVHPVQHSAREQINAQVLNTAS
                     QKIESLAQQRQWHDYRYTFKVYIPSQIATAAPCTTSPGVTLTSPAEIALNRMNFTVSC
                     PQSWQMNVAVRPDVLVPVVMAKSLVARDTPLTANDVELKPYNVSAQRRDVLMELDDAI
                     GLSSKHALQPGRPITKEELVSPVLVERDQPVMIVYQSAGITASMPGVALKNGRKGEMV
                     KVRNVSSQRVISARVAESGVVTTVSAE"
     gene            1144..1422
                     /gene="flgM"
                     /locus_tag="PN977_004855"
     CDS             1144..1422
                     /gene="flgM"
                     /locus_tag="PN977_004855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000705216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis anti-sigma factor FlgM"
                     /protein_id="HDI6044782.1"
                     /translation="MKITPTMPGNRPTATMAGSSQPRKSTATTATTLSADDITQAGLQ
                     SAQQTLNDQQESDIDSDKVAQMQAMLASGSLQVDTEQLASDMLSFFQN"
     gene            1435..1863
                     /locus_tag="PN977_004856"
     CDS             1435..1863
                     /locus_tag="PN977_004856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001329715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlgN"
                     /protein_id="HDI6044783.1"
                     /translation="MSIRLQQVKALLQGIRDDDALYDSLRELLQRQRMCMIRRASEDL
                     LAVNDEITQHYEQLHGHSHQRHSLLKMLNVSVNRDGLAQVFAWLPAVQKAAAQQLWQR
                     LEQKAERCKAYNDKNGELLIRQYEFIQSFLGGETDFLYQE"
     gene            1991..2920
                     /locus_tag="PN977_004857"
     CDS             1991..2920
                     /locus_tag="PN977_004857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000008086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-N-methylase"
                     /protein_id="HDI6044784.1"
                     /translation="MSFIERYEPEYVRNFMTQYPDSPLYVRKVWKNEIRQSLALTDIE
                     SCKAVLNDARAFDLQLTYRPVEDNAAELSARDAIVNQAILSTLTLPDLTPELSLYAVG
                     ILLSRASKMPGRDGDILARLTTLPQALGDHAQKGTLQAQFAQLPPVPQLARQLVTLLG
                     SFAFDWSILPESPRKASLPLQVTLLTLHDANSEALLQQQLKVQWQTTWQQHFAAAPWM
                     MRNWLIYRVYHDVIGQADGADYCPLVCDFYLIRTLISLWTLDGSPLRKEDIFALFAVF
                     ERWRESENAAAIRQQLQSLCAAEPLLSAFSLLT"
     gene            3099..5543
                     /locus_tag="PN977_004858"
     CDS             3099..5543
                     /locus_tag="PN977_004858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001412594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HDI6044785.1"
                     /translation="MPTPTIIKTLATQREKPSVSVITPTWNRSAFLPYLLYMYRYQDY
                     PADRRELIILDDSPQSHQHIIDRLTNGTPEAFNIRYIHHPEKLPLGKKRNMLNELARG
                     EYILCMDDDDYYPADKISYTIAMMQKNRALISGSDQIPIWYSHINRIFQSRSFGPHNI
                     LNGTFCYHRNYLKKHRYDDDCNLGEEKSFTDNFSVNPLQLPGERTILCISHSHNTFDK
                     DFILGASTPVNATLTDIVRDPLLRNAYLSLHNATHHQAIQYQAIDQIVLLNLDKRPDR
                     LQQIREELTLLHIPPEKITRLAASENENGQRGRQQSHLQALRLAQQRGWQNYLLLEDD
                     AVILKQEKHIQVLNALLASLAKIPWQVMILGGEISQGTMLKSLPGLVHARDCRKVCAY
                     LVNRRYYPQLAQQMNNDEHSLEDGWQPLLRTDKWLACYPSLCYQRPGFSDIEKKATDN
                     INHYFNKLPVATKPSTLPIADTIGFFMETSFHYALYRPIITALQAQGQSCTLVINDRV
                     FKPFLDEMLATLKSIDDPQLKGMRLSEMQAHGQRVKCLVSPYHTPVLSGLSPLNIRAM
                     YGLAKETWNHADWNRFYQRILCYSHYSQRALAHFGSAIVVGNPRFDAWHNATFDRALP
                     ENIKLDERKPTVLYAPTFGALSSLPHWAEKLGRLSDDVNLICKLHHGTCSRPEEAASL
                     ALARRHLKQRTDSARHTLALLAKADYVLTDNSGFIFDAIHVDKRVILLDFPGMATLLD
                     GEKSYSTPESADQRIREILPVAHDVAELRYLLSEAFDWGAVQARLTKIRHHYCDAFMD
                     GKAGERAAMVIVEALG"
     gene            5869..>6230
                     /locus_tag="PN977_004859"
     CDS             5869..>6230
                     /locus_tag="PN977_004859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000220508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6044786.1"
                     /translation="MTYDKKVTSGTTSQKQNNIVTQKISRPKELFLLFWVDESGDSRR
                     SIFEEACLTRYFNILYSPEYKKETQIVYHLSINTFNQIKEILEIFINKNGGITKAKVK
                     EVSLFSHGGPIHGPTTSDS"
BASE COUNT         1566 a         1732 c         1561 g         1371 t
ORIGIN      
        1 cgcgcctgct cgctgttcag ctccacggtg ttgccatctt ccgacggctg catcggcacg
       61 cggtactgga catccaccgc cgcgttattt gcccgttgca tctccctggc aaaatcaata
      121 tctttagcct tgaaatttgg cgtatcgacg ttcgccagat tcgcagtcag tagctcggtc
      181 ctctcaagac gtaacttcac tgcctgcgga tgcactccca atgcctgttg aaaactgatc
      241 cccatttctt gcgtcactcc cgcttcaatc cgcatacgct aagcaatcgc tgtgccaatt
      301 tttaagtgac tgatgagaaa gggtttatga gttttcaatt ttgcaggcgg aagttttatt
      361 tcctcctggc gctggcctta ccgggctacg ccgccgtcca tcccgtgcaa catagcgcgc
      421 gggaacaaat caacgcccag gtactgaata ccgcgagcca aaaaattgaa tcgctggcgc
      481 agcagcggca atggcatgac tatcgctaca cctttaaggt ctatattccg tcgcagattg
      541 cgaccgccgc gccctgtaca acgtcacccg gcgtcacgct gacgtcgcct gcggaaatcg
      601 cccttaaccg gatgaacttc accgtcagtt gcccgcaaag ctggcagatg aacgtcgcgg
      661 tgcgcccgga tgttctggta ccggtggtga tggcaaagtc actggttgcc cgcgatacgc
      721 cgctcacggc aaatgatgtg gagctgaagc cctacaacgt cagcgcccag cgtcgcgatg
      781 tgttgatgga gctcgatgac gcaattggtt taagcagtaa gcacgcgttg caaccgggca
      841 gaccaatcac caaagaagag ctggtttcgc cagtgctggt cgaacgcgat cagccggtga
      901 tgattgttta ccagagcgcg gggatcaccg cctcgatgcc cggcgtggcg ctgaaaaatg
      961 gtcgcaaagg ggaaatggtg aaagtacgta acgtcagcag ccagcgggtc attagcgcga
     1021 gggtggcgga aagcggcgtg gtgacaacgg taagcgcgga gtaatttgcg caggcgatta
     1081 aagttttgcc gcctgttaac cgctttaccg gagtaaccca cccaataccg agacgaggtt
     1141 acgatgaaaa tcaccccgac gatgccaggc aatcgcccga cagccacaat ggcaggctcc
     1201 agtcagccgc gcaaaagcac ggcgaccacc gccacgacgc tgagcgccga cgacattacc
     1261 caggcgggat tgcagtctgc ccagcaaacg ctgaacgacc agcaagagag cgatatcgac
     1321 agcgacaaag tggcgcaaat gcaagcgatg ctggcgtccg gcagcctgca agtggacacc
     1381 gaacagttag ccagcgacat gctgagtttt ttccagaact aagaggccga tgaagtgagt
     1441 atccggttac aacaggtgaa ggcgttgttg cagggcattc gtgacgacga cgcactgtac
     1501 gattccctgc gcgaacttct ccagcgtcag cggatgtgca tgattcgccg cgccagcgaa
     1561 gatctgctgg cggtaaacga cgagataacg cagcactatg agcagttgca tgggcatagc
     1621 catcagcggc acagcctgct gaaaatgctt aatgtcagtg taaatcgtga cggtctggcg
     1681 caggtttttg cctggcttcc ggctgtgcaa aaagcagcag cacaacagtt gtggcaacgc
     1741 cttgagcaaa aagcggaacg ctgcaaagcc tacaacgaca aaaacggcga gctgttgatt
     1801 cgccagtatg aatttattca gtctttttta ggcggcgaaa cagattttct gtaccaggaa
     1861 taacgacaat tccttccccc atcgcggaaa cggattttcc gcctgattct caatcccttg
     1921 tttttgatta caaaaattcc cggcacgctt attgcacatt ccgcaaagta ataaagactg
     1981 gaagccacct atgagtttta ttgaacgtta cgaaccggaa tacgtgcgca attttatgac
     2041 gcaatatccc gattcgcctt tgtacgtccg caaggtctgg aagaatgaga ttcgccagag
     2101 cctggcattg accgatattg aaagttgcaa agcggtgctc aacgatgcca gggcctttga
     2161 tttacagctc acctaccgcc ctgtggaaga caatgccgcc gagctatccg cgcgcgatgc
     2221 gatagtaaac caggccattt tgagcaccct tacgctcccc gatttaaccc cggagctttc
     2281 cctgtatgcc gtcgggattt tgctctcccg cgccagtaaa atgccgggca gagacggaga
     2341 tattcttgcc cgcttgacga cgctgccaca ggcgctgggg gatcatgcac aaaaaggcac
     2401 gctacaggca cagttcgccc aactcccacc cgtgccacaa ctggcgcgcc aactggtgac
     2461 gctgctcggg agttttgcgt ttgactggtc gattttgccg gaaagcccgc gcaaagcctc
     2521 gctaccgttg caggtgactt tgctgacgtt gcatgacgcc aacagcgaag ctttgttgca
     2581 acagcagctt aaggtgcagt ggcaaaccac ctggcaacag cattttgccg ccgcgccgtg
     2641 gatgatgcgc aactggctga tttaccgcgt ttatcacgac gtgattgggc aggcagacgg
     2701 agcagattat tgcccgctgg tgtgtgattt ttatctcatc cgcacgttaa tcagtttatg
     2761 gacgctggat ggttcaccgc tgcggaagga ggatattttc gccctttttg cggtgtttga
     2821 acgctggcga gagagtgaaa atgctgcggc gattcgccag cagctccagt cgttatgtgc
     2881 ggcagagccg ctgctgtcag cattttcgct gctgacgtga gtcgctctgt gcgcgctttt
     2941 cccctccccc taaccctctc cccaaagggg cgagggaacc gatcgagcac ggtgttaaaa
     3001 tcggcgctgg ttttctccct ctccctgtgg gagagggtcg gggtgagggc atcagcacgc
     3061 atattcatca cattcaccca ttaactcacc caaacaccat gccaacacca accattatca
     3121 aaacactcgc cacccagcgc gaaaaaccgt ccgtcagcgt catcacgcca acctggaacc
     3181 gcagcgcatt cctgccgtac ctgctttata tgtaccgcta tcaggactac ccggcagacc
     3241 gccgcgaact catcatcctc gacgattccc ctcaaagcca tcaacacatc atcgaccgcc
     3301 tgaccaatgg cacaccggaa gccttcaaca tccgctacat ccatcacccg gaaaaactgc
     3361 cgctgggcaa aaaacgcaat atgctcaacg aactggcgcg cggcgaatac atcctctgca
     3421 tggacgatga cgactactac ccggcagata aaatctctta caccatcgct atgatgcaaa
     3481 aaaaccgggc gctcatctcc ggttccgacc agatccccat ctggtacagc cacatcaatc
     3541 gcatctttca aagccgcagc ttcggcccgc ataacatcct caacggcacc ttttgctatc
     3601 accgtaacta cctgaaaaag caccgctacg atgacgattg caatttaggc gaagagaaaa
     3661 gtttcaccga caatttcagc gttaacccgc tacaactgcc cggcgagcgc acgattttgt
     3721 gtatctcgca cagccacaac acctttgata aagactttat tctcggggcc agcacgccag
     3781 taaacgcgac gcttacggat atcgtccgcg atccgctgct ccgcaatgct tatctgagtc
     3841 tgcataacgc cactcatcat caggcgatcc agtatcaggc gatcgatcag atcgttctgc
     3901 tgaatctgga taaacgcccg gatcgcttac agcagatccg tgaagaactc acattgctgc
     3961 atattcctcc tgagaagatc acccgccttg ccgccagtga aaatgaaaac ggtcaacggg
     4021 gccgccagca atcacacttg caggcactgc gtctggcgca acaacgcggc tggcaaaact
     4081 acctgctgct ggaagatgac gcggttattc tcaaacagga gaaacatatt caggtgctga
     4141 acgcgctgct ggcgagcctg gcgaaaattc cctggcaggt gatgatcctc ggcggcgaaa
     4201 tttcgcaggg aacgatgcta aaaagtttgc ctgggctggt gcacgcgcgg gactgccgca
     4261 aagtgtgcgc ttatctggtg aaccgccgct actacccgca gctggcgcag cagatgaaca
     4321 acgatgagca ttcgctggaa gacggctggc agccgctgct gcgcaccgac aaatggctgg
     4381 cctgttaccc gagcctttgt tatcagcgac cgggctttag tgatatcgag aaaaaagcga
     4441 ctgataacat taaccactac ttcaataagt tacccgtagc caccaagcca tctacattac
     4501 ctatcgccga taccatcggc ttttttatgg aaacctcatt ccactacgcg ctctaccgcc
     4561 caatcatcac cgccctgcag gcgcaggggc agagctgtac gctggtgatc aatgaccgcg
     4621 tatttaaacc atttctggat gaaatgctgg cgacgctaaa aagcatcgac gatccgcagc
     4681 tcaaaggaat gcgcttatct gaaatgcagg cacacggtca gcgagtaaaa tgcctggtca
     4741 gcccctatca cacgcctgtg ctcagtggcc tttcccccct gaacattcgc gccatgtacg
     4801 gcttagccaa agaaacgtgg aaccacgccg actggaaccg tttttatcag cgcattttgt
     4861 gttacagcca ctacagccag cgggcgctgg cacatttcgg cagcgcaata gtggtcggca
     4921 acccgcgctt cgacgcctgg cataacgcca cgttcgaccg cgcgttgccg gaaaatatca
     4981 aactggacga gcgtaaaccc accgtactct acgccccaac gtttggcgca ttaagctccc
     5041 tgccccactg ggcagaaaaa ttggggcgct taagcgatga tgtgaacctg atttgcaaac
     5101 tgcaccacgg cacctgctcg cgcccggaag aagctgcatc gctggccctg gctcgcaggc
     5161 atttaaaaca gcgcaccgac tccgcccgtc acacgctggc gctgctggca aaggccgatt
     5221 acgtgctgac cgataacagc ggctttattt tcgatgctat tcacgttgat aagcgggtga
     5281 tcctgctcga ctttccgggc atggcaacgt tgctcgacgg tgagaaaagt tactccacgc
     5341 ccgaaagcgc cgaccagcga atccgcgaaa tattgccggt ggctcatgat gttgcggaac
     5401 tgcgttattt gttgtcagag gcgtttgact ggggtgcggt gcaggcgcgg cttacgaaga
     5461 ttcgtcatca ctattgcgat gcgtttatgg atggcaaggc gggagaacgg gcagcgatgg
     5521 tgattgtgga ggcactaggg taaaacgcgc aagtgcctga tgcgcttcgc ttatcaggcc
     5581 tacgcgacct tgcaatgtat tgaatttgta aggatttgta ggccggttaa ggcgtttacg
     5641 ccgcatccgg aacgaacaac gcacttaact accctctgat ttcaaactca tcgctttatg
     5701 actggtgaat cggtaaaagt cgtagattgt gggggagcga tctttatgaa ttctgaaaaa
     5761 acctgaatct tgctaaacat attgcttatc ataaatatag gttacacact ataacaatta
     5821 ttgcgtaatt taacattttg ttaaaattga tagggcataa acgataacat gacttatgat
     5881 aaaaaagtaa cttccggtac aacaagccag aaacaaaaca acattgtcac tcaaaaaata
     5941 tctcgcccaa aggaattgtt tctattattt tgggttgatg agtccggtga tagccgacgt
     6001 tctatttttg aagaagcatg tctgacgcgt tatttcaaca tcctttactc ccccgagtat
     6061 aaaaaagaaa cacagatagt ttaccattta tcaattaaca catttaatca aatcaaagaa
     6121 atattagaaa tctttataaa caagaatgga ggtataacca aagcaaaagt taaagaagtc
     6181 agtttattct cacatggggg gccaattcat ggtcctacaa cctcagactc
//
LOCUS       DAMZFT010000217         6212 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.217, whole genome shotgun sequence.
ACCESSION   DAMZFT010000217 DAMZFT010000000
VERSION     DAMZFT010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6212)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6212)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6212
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.217"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            147..1034
                     /locus_tag="PN977_004860"
     CDS             147..1034
                     /locus_tag="PN977_004860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001750275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDI6044787.1"
                     /translation="MGDSISSWFKQCWSDIQEAFNGGIAGTGKLLINWSPAGLLYKAF
                     AAALKYLGVDLPAKFTDFGGHLVDGLINGIKNKWESLKSSVTGMGDSISGWFSEKLGI
                     HSPSRVFMGFGDNIAQGAAIGLQRSTPIAALAGQRMASELLPRMPVSIQGPEIRDNTS
                     GVRFSMPLPDINGFMSSAKNAIGSVINSLSSMPAIPISTRASTLPGQRLANEMTPDVP
                     RIPSPEILAAGYSGRGAAATGGGTSGGIQVSFNPQFFLNGRETTAPAGLTGALNMSLH
                     ELEKMLERLLAQKQRKEYR"
     gene            1034..1918
                     /locus_tag="PN977_004861"
     CDS             1034..1918
                     /locus_tag="PN977_004861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001423226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDI6044788.1"
                     /translation="MFAVLGDIEFDLITYWDGFEATFGVDYAEHARIGGKPGLQFVGD
                     RLDEIQISLVFHQHYCAPDVELARLRTTMKAHQALALVFGNGDYRGWFVITDVTATSE
                     QTDSTGNVLAVSATVSLREYTGDPKNPLQPPAIRTKLPGVGAVSGAIPSPSGVAQFIR
                     NGVNYAKQAQSVLQTTISAVRVTQKMKDNPVVALTRVPGLMSGLGNISGALGKSVPAF
                     NALSESMPDAISLARTASEAATYVQQAQSALSGVDKRNIAGALDTVSGQLNAASTAFN
                     RMSPGLSAMAARILTRSV"
     gene            1918..2130
                     /locus_tag="PN977_004862"
     CDS             1918..2130
                     /locus_tag="PN977_004862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000478222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail protein X"
                     /protein_id="HDI6044789.1"
                     /translation="MFLEHVTRDGERWDSLAWQYYGDPLGYPRIIAANPHVAITPVLP
                     SGLLLLIPVIEAEEARTEEDIAPWLR"
     gene            2118..3272
                     /locus_tag="PN977_004863"
     CDS             2118..3272
                     /locus_tag="PN977_004863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001545521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage late control D family protein"
                     /protein_id="HDI6044790.1"
                     /translation="MAEINSTAQVTSALTSVSDVLTPVFTLWYLQKNITSDIAPYVTR
                     VTWSDNIKNESDTIEVELDDTDGRWLDKWYPGKGDTLTLKMGYQGEKLLSCGTFSIDE
                     IEVSSPASVVSIRGVATSVNSALRTKTSRGFENTTLAAVAGRIARKHRLKLVGSIESI
                     RIDRVTQYAETDVGFLRRLASEYGYAVKVVSDQLIFSHLATLRSQEPVRQLKPQDVAR
                     FSLRDTINRVYKSAKVKHQKSSSKKLIVYEADGGTRESDKKLKGGKVTSADSLKVNSR
                     VSDPDSARIKADSALARHNEYQQNGSLTLTGTPQLTAGNKIELVGFGQLSGPWLITTA
                     RHAFDRNSGYTTELEVARGPVTRGKKQKTQKLTVYHPDGSTSTVIKEKKK"
     gene            3269..3865
                     /locus_tag="PN977_004864"
     CDS             3269..3865
                     /locus_tag="PN977_004864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016236042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein V"
                     /protein_id="HDI6044791.1"
                     /translation="MTGVTRQVGTVSAVDADRVQARVRLPECDNLRTNWLNVLQRNTQ
                     DNKDYWLPDVGEQVEVLLDANGEDGVILGAVYSDVDKPPFSDKNIRGTRFADGAEYSY
                     NRKTHTLTIRGGIEHIVIECGADVVLKTQKATIDAPETELTGDLRVRGKLIYEGGMAG
                     SGGKGVTATIHGNIEIKGNAHATGSMLSDGENSSHHSH"
     gene            3920..4267
                     /locus_tag="PN977_004865"
     CDS             3920..4267
                     /locus_tag="PN977_004865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000859115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPW/gp25 family protein"
                     /protein_id="HDI6044792.1"
                     /translation="MKTTSVFWQPALQAPGEIVRGLDDIRQAIQIILRTPRGSDPHRP
                     EFGSNLHLYIDWPVDRAIPHVVRESVDAIRRWEPRCQLMSVKPAVDGEHLTLRVSWKG
                     SDGQTRTQELLWR"
     gene            4258..5361
                     /locus_tag="PN977_004866"
     CDS             4258..5361
                     /locus_tag="PN977_004866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012905229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HDI6044793.1"
                     /translation="MALTEPDFIERDADKITAEMIAKYEADTGKTLYPAQAERLLIDL
                     WAYREMLVRVAVQEAAKQNLVAFAREPMIDYLGELVGVYRLAAQPATTTLQFSVDEAL
                     AIDVLIPAGTRVSASDSVIFATDTDVVLKAGLLLVNVTSTCTEPGTAGNGWQPAQVSQ
                     LLDEIDNVDLLVSNLTASSGGSEQEDDDRLRERIRLAPESFTNAGSRGAYRFHAMQAH
                     PNIVDVAVLSPVPGTVDLYPLLSTGLPDGGVLTLVESFCSDEKVRPLTDTVRAKTPVK
                     VDYTIEARITIYRDQDARSVKDAANSAIQNWVASRAATLGRDIVPSQIISALSVSGVY
                     QVELVTPALRVVAENEWANCTAITLNMSGVSDD"
     gene            5354..5932
                     /locus_tag="PN977_004867"
     CDS             5354..5932
                     /locus_tag="PN977_004867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000852583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein I"
                     /protein_id="HDI6044794.1"
                     /translation="MTEPLQLPPPLEGDISLRTLGRLAGRLDNIDLSVLMVFLVDIVD
                     SSALPWLGEQFSLSGDGWELAESDDVRRMLIKAAIELHRYKGTPWSIREVIRRFGFGE
                     VDLIEGTGRLSYDGNRSYNGLFVHGDAAAWAVYRVILKQPITNDQAAMLRQTLAAFAP
                     ARCHLASLEYQSVAIRYNNTAIHDGSYNHGSS"
     gene            5935..>6212
                     /locus_tag="PN977_004868"
     CDS             5935..>6212
                     /locus_tag="PN977_004868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000527459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber protein"
                     /protein_id="HDI6044795.1"
                     /translation="MGNLNETEKWEENIYQLETSDPVLGGADGISNRAPRQLANRTKW
                     LKKKTEEAAQSLAEHVRSRNHPDATLTAKGFTQLSSATNSTSETLAATP"
BASE COUNT         1461 a         1637 c         1788 g         1326 t
ORIGIN      
        1 tgctctataa agcctttgcg gctgcgctga aatatttcgg cgttgctctg ccggcaaagt
       61 tcaccgactt cggtggccat cttgtcgacg gtctgattaa cggtatcaaa aacaaatggg
      121 ggtcgctcaa atccagtgta accggaatgg gtgacagcat cagtagctgg tttaaacagt
      181 gctggtctga cattcaggag gcctttaacg gcggtatcgc gggaactggt aagctgctga
      241 ttaactggtc gccggcaggt ctgctctata aagcctttgc agctgcgctg aaatatcttg
      301 gtgttgatct gccggcaaag ttcaccgact tcggtggcca tcttgtcgac ggtctgatta
      361 acggtatcaa aaacaaatgg gagtcgctca aatccagtgt aaccggaatg ggtgacagca
      421 tcagtggctg gttcagtgaa aagctgggta ttcattcgcc gagccgcgtg tttatgggct
      481 ttggtgacaa tatcgcgcag ggggccgcca ttggcctgca gcggagcact ccgattgcag
      541 ctctggccgg gcagcgaatg gccagtgaac tgctccccag aatgcccgtg agcattcagg
      601 ggccagaaat acgggataac acttcaggtg ttcgcttcag tatgccgttg cccgatatca
      661 acgggtttat gtcgtctgct aaaaatgcga tcggatccgt aatcaatagt ttgtcttcca
      721 tgcccgctat tccgatctcc acccgggcat cgactttacc aggtcaacgt ctggcaaatg
      781 agatgacacc ggatgttccc cgtatcccct cgcctgaaat cctggctgcc ggatattcag
      841 gccgtggtgc agctgcaacc ggcggtggaa cgtctggtgg tatccaggtc agctttaatc
      901 ctcagttttt cctcaatggc agggaaacca cagcgcctgc aggactgact ggtgccctga
      961 atatgagtct gcatgagctg gaaaaaatgc tggagcgtct gctggctcag aaacaacgta
     1021 aggagtaccg ctgatgtttg ccgtactggg tgatattgag tttgatctga ttacctactg
     1081 ggacggcttc gaggccacgt tcggcgtcga ttatgcggag catgcccgca tcgggggtaa
     1141 gcctggcctg cagttcgtcg gcgacaggct ggacgaaatc cagataagtc tggtcttcca
     1201 tcagcattat tgtgcacccg atgtggagct ggcgagactg cgaacaacca tgaaagccca
     1261 tcaggcactg gcgctggtct tcggcaacgg tgactatcgt ggctggttcg tgattaccga
     1321 tgtgaccgca accagcgagc agacagacag caccggtaac gtgctggctg tcagtgccac
     1381 cgtgtctctc cgggaataca ccggtgatcc gaaaaatcct ctgcaaccac cggcaatacg
     1441 cacgaagctc ccgggtgtcg gggcggtctc cggtgccatt ccttcacctt caggggtggc
     1501 gcagttcatc cgcaacggcg tcaactatgc gaaacaggcg cagtctgtac tccagaccac
     1561 tatcagcgcc gttcgggtga cacagaaaat gaaggataac cccgttgtcg cactgacccg
     1621 tgtgccgggg ctgatgagcg gactgggtaa tatctccggg gctctgggga aaagtgttcc
     1681 ggcgtttaac gcactctctg aatccatgcc cgatgccatc agtctggcca gaacagccag
     1741 cgaagcagcc acgtatgtac agcaggcaca gtctgcgctg agtggtgtgg acaaaagaaa
     1801 tattgcaggt gctctggata ccgtttccgg gcagcttaac gccgccagca cagcattcaa
     1861 ccgcatgtct ccgggattaa gtgcaatggc cgccagaata ctgacgagga gtgtgtgatg
     1921 tttcttgaac atgttacccg tgacggagag cgctgggatt cgctggcatg gcagtactac
     1981 ggtgacccgc tgggctatcc ccggattatt gccgccaatc cgcacgtggc catcacgccg
     2041 gtgctgccct ccgggctgtt gttactgatc ccggtgattg aggctgaaga agcccgtaca
     2101 gaagaggata ttgccccatg gctgagataa acagcactgc gcaagtcaca tcagcgttaa
     2161 ccagcgtcag cgatgtgctg acaccggtgt tcactctgtg gtatctgcag aaaaacatca
     2221 cctctgatat cgcgccttat gtcacccgtg tgacctggag cgataacatc aaaaatgagt
     2281 ccgataccat tgaggtggag ctggacgaca ccgatggccg ctggctggat aagtggtatc
     2341 cgggcaaggg tgacacgctg acgctgaaaa tgggctatca gggagagaag ctgctgtcct
     2401 gcggtacgtt ctctatagac gagatcgaag tgagttcgcc cgcttccgtt gtttctatcc
     2461 gtggggtggc cacctcggtt aacagtgctc tgcggactaa aaccagtcgt ggttttgaga
     2521 acaccacgct ggcagctgtt gcggggcgga ttgccagaaa gcaccgactg aaactggtgg
     2581 gcagcattga gtccatcaga atcgaccggg tgacccagta tgctgaaacc gacgtgggtt
     2641 ttctgcgccg gctggccagc gagtatggtt atgcagtgaa agtggtcagt gaccagctga
     2701 ttttttctca tctggccaca ctgcgcagtc aggagccggt caggcagtta aaaccgcagg
     2761 atgtggcccg cttttccctg cgtgacacca tcaaccgggt ctataaatct gcaaaggtaa
     2821 aacaccagaa aagcagcagt aaaaaactga tcgtctacga agctgatggt ggtacccgtg
     2881 aaagcgacaa aaagctcaaa ggtggtaagg ttaccagcgc tgactcactt aaagttaaca
     2941 gccgcgtcag cgacccggac agtgcccgga ttaaagcgga ttcagcactg gccagacata
     3001 acgaatacca gcagaacggc tccctgacgc tgacgggaac acctcaactg acagcaggca
     3061 acaaaattga actggtgggt tttgggcagt tatccgggcc atggctcata accactgccc
     3121 gccatgcgtt tgaccgtaac agcggctaca ccacagagct ggaagtggca cgggggccag
     3181 tcacaagagg gaaaaaacaa aaaactcaga aactcacggt ttatcacccg gatggcagta
     3241 catcgacggt gattaaggag aagaaaaaat gactggtgtc actcgtcagg tcggtacggt
     3301 cagtgccgtt gatgccgaca gggttcaggc ccgcgttcgt ctgcctgaat gcgataacct
     3361 gcgcacaaac tggcttaacg tgctgcagcg caatacccag gataacaaag attactggct
     3421 ccctgacgtg ggggagcagg ttgaggtgct gctcgatgcc aacggcgagg atggtgttat
     3481 tctgggcgcg gtgtattcag acgtcgataa accaccgttc agtgacaaaa atatccgggg
     3541 aacccggttt gctgatggtg cagagtacag ctacaaccgg aagacgcaca ctctgaccat
     3601 ccggggcggc attgagcata ttgtcattga gtgtggtgct gatgtggtat tgaaaacaca
     3661 gaaagccacg attgacgcac cggaaaccga acttaccgga gatctgcgtg tcaggggaaa
     3721 gctgatttac gaaggaggca tggcgggttc tggtggtaaa ggtgttaccg cgaccatcca
     3781 tggcaatatc gagattaaag ggaatgccca tgccacgggc agtatgctgt ctgatggcga
     3841 aaactccagc caccactccc actgagcttc ttaaacgcct ttaatatcag cgttcccgca
     3901 cgggggcaat actgccccca tgaaaacgac ctcagtattc tggcaaccag ccctgcaggc
     3961 tcccggcgaa attgtccggg ggctggatga tatccggcag gcgattcaaa ttatcctgcg
     4021 gactccccgc ggcagcgacc cgcatcgccc ggagttcggc agcaatctgc atctttatat
     4081 cgactggcct gtagaccggg ccattccgca tgtggtgcgc gaatccgtcg atgccatccg
     4141 gcgctgggag ccccgctgcc agcttatgtc agttaaaccc gccgtcgacg gcgaacatct
     4201 tacgctccgg gtgagctgga aaggctcaga cggacagacc cggactcagg agctgctatg
     4261 gcgctgacag aacccgattt tattgaacgc gatgccgaca aaatcacggc agaaatgatt
     4321 gcgaagtatg aagcggatac cggcaaaacg ctgtacccgg cacaggcaga acgtctgctg
     4381 attgatctgt gggcctatcg cgaaatgctg gtcagggttg cggtacagga ggcagcaaag
     4441 cagaatctgg tcgcctttgc ccgtgagccg atgattgatt acctcggtga actggtcggt
     4501 gtataccgtc ttgccgcgca gcctgccacc accacgctcc agttctccgt agatgaggca
     4561 ctggccattg atgtgctgat cccggcaggc acccgcgtca gcgcttccga cagcgttatt
     4621 tttgccaccg atacagatgt ggtactgaag gccggattgc tgctggtcaa tgtcacgtcc
     4681 acctgtaccg aacccggtac cgctggtaac ggctggcaac ctgcgcaggt cagtcagtta
     4741 ctcgatgaga ttgataacgt cgacctgctg gtgagcaatc tgacggccag ttccggcggt
     4801 tcagaacagg aagacgatga caggctccgg gagcgtatca ggctggcccc ggagtcattc
     4861 accaatgccg gaagccgtgg cgcataccgc tttcatgcca tgcaggccca tcccaacatt
     4921 gtcgatgttg ctgtgctttc cccggttccc gggaccgtag atctgtatcc gctgctcagt
     4981 accggtctgc cggacggcgg tgttctcacg ctggtagaga gtttctgctc tgatgagaaa
     5041 gtcaggccac tcactgatac agtgcgggct aaaacaccag tgaaggtgga ttacaccatt
     5101 gaagccagga ttacgatcta tcgtgatcag gatgccaggt ctgtaaaaga tgccgctaac
     5161 agcgccatac agaactgggt ggcatcacgt gccgccacgc tggggcgtga tattgtcccc
     5221 agccagatta tcagtgcatt gtccgtttcc ggggtgtacc aggttgaact ggtgacaccg
     5281 gcactgcggg tggtggcaga aaacgaatgg gcaaactgta cggcaatcac tcttaacatg
     5341 agtggagtgt ctgatgactg agccattaca actcccgcca ccgcttgagg gtgatatcag
     5401 tctcaggacg ctgggaagac tggcaggacg gctggataac atcgacctga gcgtactgat
     5461 ggtctttctc gtcgatatcg tcgacagttc cgcgctgcca tggctgggcg agcagttctc
     5521 actgtctggc gatggctggg agctggcgga atcggacgat gttcgccgca tgcttataaa
     5581 agcagccatc gaactgcacc ggtataaagg gacgccgtgg tcaatccggg aagttatccg
     5641 ccgttttggc tttggtgaag tggatctgat tgaaggcaca ggtcgtctca gttacgacgg
     5701 caatcgcagc tataacggac tgtttgttca tggagatgca gccgcctggg cagtttatcg
     5761 cgttattctg aaacagccca ttactaacga tcaggccgcg atgctgcgtc agacgctggc
     5821 tgcatttgca ccggcccgct gccatctggc cagcctggag tatcagtctg tggccattcg
     5881 ctataacaat accgccatcc atgatggcag ttataaccac gggagcagtt gattatggga
     5941 aacctgaatg aaacagaaaa gtgggaagaa aatatctatc aactggagac atcagatccg
     6001 gttctgggtg gtgcagacgg gatatcaaat cgagcccccc ggcaactggc aaacaggacg
     6061 aaatggctga agaagaaaac ggaggaagcc gcacagtcac tggctgaaca cgtacgttcc
     6121 cgtaaccacc cggacgcgac actgacagct aaggggttca cccaactgag cagtgccacc
     6181 aacagcacct ctgaaacgct ggccgccaca cc
//
LOCUS       DAMZFT010000218         6206 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.218, whole genome shotgun sequence.
ACCESSION   DAMZFT010000218 DAMZFT010000000
VERSION     DAMZFT010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6206)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6206)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6206
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.218"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(96..1037)
                     /locus_tag="PN977_004869"
     CDS             complement(96..1037)
                     /locus_tag="PN977_004869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HDI6044796.1"
                     /translation="MSGKVCVFGSFNFDMVARVDHFPVPGESLVACGSMTSAGGKGAN
                     QATAALKAGANVHYIGKIGNDTFGHFARRHLKGVGFNAVTLLVAEEIPTGNALIYVAG
                     NDAENMIAVDPGANMTVTDDEIAGCIPAIGCADVVLVQLENNLSAIEQVIDAGKQAGA
                     LVILNPAPWQPVEHAVLSKVDLLTPNATEAGLMTGRRVDSLTAAAEAADVLHAQGARN
                     VIITLGASGALLSEHGVKSPIPCFPSHPRDTTGAGDAFNGALAARLACGEPLQAAARF
                     AAAYAAVSVEKQGASSLPEYLEAQERLLRAAADYEMA"
     gene            complement(1049..1738)
                     /locus_tag="PN977_004870"
     CDS             complement(1049..1738)
                     /locus_tag="PN977_004870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HDI6044797.1"
                     /translation="MRYYLSPSLMCMDMMKLTEQLRFLNSKADRLHVDIMDGHYVKNL
                     ALSASFVAQIRPYTSLPIDVHLMVEAPASFIPALLDAGADAFSLHPETICREAFRVIN
                     MLRQAGKEVGMVLNPATPVESIQHYLHLLDKVTVMTVDPGYAGQPFIPEMLAKITQLH
                     QLKETGSLRFLLEVDGSCNRNTYRALLGAGAQILVMGSSGLFRADMPLELAWETMSRE
                     LSAALHSPELV"
     gene            complement(1848..2648)
                     /locus_tag="PN977_004871"
     CDS             complement(1848..2648)
                     /locus_tag="PN977_004871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HDI6044798.1"
                     /translation="MLPLERHRLIVELLGQHGVMRVNEIAQATRVSRETIRRDLSELE
                     RKGILTRSHGGALAAENPLPATRAAASALPVEDSQGSFQHRTLLHSEGKMRVARKALQ
                     FLQPGQTIVLDGSSTSWFLARQMPEMALTVITPSIRILQTLMSRRSLHLVGLGGDFSP
                     TEESFFGETASRTLREYAIDTLFFSCQGLERDSGLYAGTQAHAALLKQMLLAARQTVA
                     LVDGSKLGRTGVARIGGLGELDCLVTERFVDPLLEKEMTWHNVSLQIA"
     gene            3011..3760
                     /gene="deoC"
                     /locus_tag="PN977_004872"
     CDS             3011..3760
                     /gene="deoC"
                     /locus_tag="PN977_004872"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HDI6044799.1"
                     /translation="MKAIKNKGQDKTMNETTRRFARLVDLSAVQATSTEADVRACAEL
                     AARYNIISVHVLPCWTRFLSTLLPQQGTGEVMIGGPVGFPGGGHTTDTKVQEVRQLIA
                     DGAREVDMVVNIGKVLSGDYDYVREDLRRVVEAAAPVPAKVILETHYLNEEQIRRVCE
                     IAVEVGMKWVKTSTGWAPTGATVEKVSIIADQLKGRIDIKGAGGIRDLATVRALYQLG
                     VRRFGMSHGAVTKVLAELEQHPERFPELNAD"
     gene            3814..5319
                     /locus_tag="PN977_004873"
     CDS             3814..5319
                     /locus_tag="PN977_004873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentose kinase"
                     /protein_id="HDI6044800.1"
                     /translation="MKDKILTIDVGTGSTRAAIVRIDGAMIGFAQREYEQTTPRAGWS
                     EQAPSLWWQAACDCIREVLYRYPETAAQIAVIGACGQMHGTVLLDDRGELVEDRALLW
                     NDKRSQPQVDAFNAREGWEKWLAHLNNPPAAAWPAFKLAWWRENHPDRWSQLAKVLMP
                     KDYINFMLTGAMATDYSEASCYFLMDSETRSWSSQACETFGLRVDQLPELKLSSDIIG
                     QVTQRAADLTGLPAGIPVVAGTSDMAASLLGSGVYEPGMASDSTGTSTLMTVVSPRPL
                     HHPLVNNLHLANAAWGGFTILDAGGDAVRWARLALADNQITHPQLLQEAAAVPVGAEG
                     LLFLPYLTGERLAEHTNSRAQFFGLQRKHRRGHLFRAVLEGVAFASWRNLRQLQKCGQ
                     YPQQMIASGGGARSSLWLEIKAAAYNLPILSTRNQENGVTGCGIIAGVGVGLYADFAS
                     GVRQTVQFDKLISPDPRLRDYYHACCELFDTLYRQSAALYDRLDALSVGPD"
     gene            5381..6154
                     /locus_tag="PN977_004874"
     CDS             5381..6154
                     /locus_tag="PN977_004874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HDI6044801.1"
                     /translation="MFAEERQEKLLGILREKRKVAVSEMCDVFNVSGATIRADLRQLE
                     EEGLLTRTHGGAVLRTRASFEQASDAREIVNLSAKERIGQRAAALVEDGDIIVLDTGT
                     TTLHIARNIRDRQNVTVVTNDYQIARELEVSPSIQTILLGGIVKKGYHCVVAINGRSI
                     LDTLNVDKAFMGTNSLSLKHGACVADIMLAETKRGMVEHASQVIIVCDYSKLNNTSLA
                     QFTAADRIDTVVVDRLPDDVQAYRNAGIQVISLDEEEHL"
BASE COUNT         1346 a         1711 c         1805 g         1344 t
ORIGIN      
        1 tccagttttt ctttttggac cgctgcgtta tcgaccttat ccgtgattag cgtactcatt
       61 gagctatcct cttaatgttt tttatacagt agggtttatg ccatttcata atcagcggcg
      121 gcacggagaa gccgttcctg tgcctccagg tactcaggta aagatgaggc gccttgcttt
      181 tcgacactga cggcggcata ggccgccgcg aatcgggctg cggcctgtag cggttccccg
      241 caggccagcc gcgccgccag cgcgccgttg aaggcatcgc ccgcaccggt ggtgtcgcgg
      301 gggtgagaag gaaagcaggg aataggggat ttcaccccat gctcgctcag cagggcgccg
      361 ctggcgccga gagtgataat gacgttgcgc gcgccctgag catgtaacac gtcggcggcc
      421 tcagccgcgg ccgtcagact gtcaacccgg cgtccggtca ttaacccggc ctccgtggcg
      481 ttcggtgtaa gtaaatccac tttgcttaga acggcgtgct caacgggctg ccagggcgcg
      541 ggattgagta ttaccaaggc cccggcttgt ttgccggcgt ctatgacctg ttcaatagca
      601 gaaagattgt tctccagctg aaccagaacc acatccgcgc agccgatagc gggaatgcag
      661 ccggcgattt catcatcggt caccgtcata ttcgcgccgg gatcgacggc aatcatgttt
      721 tcggcatcgt taccggcgac ataaatcagc gcattgccgg tggggatctc ttcggcgacc
      781 agcagcgtca cagcattgaa gcccacgcct ttcagatgac ggcgggcaaa atggccgaag
      841 gtgtcattgc caatcttgcc gatatagtgg acatttgctc ccgcttttag cgcggcggtg
      901 gcctggttcg cccctttacc tccggcgctg gtcatacttc cgcaggccac taacgactca
      961 cctggtaccg gaaagtgatc caccctggcg accatgtcga aattgaatga accaaaaaca
     1021 cagacttttc ctgacatcat tcgctcctct ataccagctc gggggagtgc agcgcggcgg
     1081 acagttcgcg ggacatggtt tcccaggcca gctcgagcgg catatcagcg cggaataaac
     1141 cgctgctgcc catcaccaat atctgcgccc cggcgcccag cagcgcgcgg taggtgttgc
     1201 ggttacagga gccatcgact tccagcaaga agcggaggct gccggtctct ttgagctgat
     1261 gaagctgcgt aattttggcg agcatttcag gaataaacgg ctgaccggca tagcctgggt
     1321 cgacggtcat caccgtcacc ttatcgagca gatgcagata gtgctggatg ctctcaaccg
     1381 gtgtggcggg attgagcacc attcccacct ctttccccgc ctgacgaagc atattgataa
     1441 cccggaacgc ttcgcggcag atggtctccg gatgcagtga gaaggcgtct gcgccggcgt
     1501 ctaacagcgc cggtataaac gaagcgggcg cttcgaccat cagatggaca tcgataggaa
     1561 gcgaggtgta gggacggatc tgcgccacaa agctggccga caacgccaga tttttgacat
     1621 agtggccgtc catgatgtcg acatgcagcc tgtcggcttt gctgtttaga aagcgcagct
     1681 gttccgtcag cttcatcatg tccatacaca tcagcgaggg agaaaggtaa taacgcataa
     1741 ggcctcattt attccggtgg tggctgaatt caggcaaagt ctggccctgc cgcggtaagc
     1801 agcgtttcga cattgcgcgt attaaccgtc acgtcatgac ggcgtactca ggcgatttgc
     1861 aggctgacgt tatgccaggt catctctttt tccagcagcg gatcgacaaa cctttcagtc
     1921 accagacagt ccagctcgcc cagtccgccg atacgcgcga cgcctgttcg ccccagcttg
     1981 ctgccgtcga ccaaagccac agtctgacgg gcggccagca gcatctgttt cagcagggcg
     2041 gcgtgggctt gcgtacccgc atacaagccg ctatcgcgct ccagcccctg gcaggagaaa
     2101 aagagcgtat caatggcata ttcgcgtagc gttcgtgagg cggtttcccc gaagaacgac
     2161 tcttcggtcg gggagaaatc gccccccagc ccgacgagat gcaacgagcg tcggctcatc
     2221 agagtttgta agatgcgaat ggagggcgta atcaccgtca acgccatctc cggcatctgc
     2281 cgggccaaaa accagctggt gctactcccg tccaggacaa tggtctgtcc cggttggaga
     2341 aactgcagtg ctttacgggc cacgcgcatt ttgccttcgc tgtgcagcag cgttctgtgc
     2401 tgaaagcttc cctggctatc ttcaactggc agggcggatg ccgccgcccg ggtcgccggc
     2461 agcggatttt ccgccgccag cgctccaccg tggcttcggg ttaagatccc tttgcgttcc
     2521 agctcgctca gatcgcggcg aatcgtttcc ctggagaccc gagtcgcttg cgcaatctca
     2581 ttcacccgca tgacgccgtg ttggcccagc agttcaacaa tcaaacgatg acgttccagc
     2641 ggcaacatag ctctctcctg aagtacataa tcagattacc tttcccttgc gcagagcgtt
     2701 gagacctcgg gaatgtccca cgaaggcaag ctatccgcag ccgaacactg tgatagcgca
     2761 cgcatttcgc ttcacttccg tcagcggttt gcgctatttc gcccgtgatt caggcataaa
     2821 caaaaacaaa caaaaataaa tgcaagattg cttttgcgcc catttatgcc cgattacgcc
     2881 tgctctgaaa ggttaacgct tatttcctgc tgctaattaa aaacaaatga aaataaaaaa
     2941 atgctaattt gatcacgtta ttttcattta atgcgttttt aaatggattt aagccaaata
     3001 tcagcattaa atgaaagcaa ttaaaaacaa aggacaggat aagaccatga atgaaacaac
     3061 ccgtcgtttt gcacgtttag ttgatctgag cgcagttcag gccaccagta ccgaagcgga
     3121 tgttcgcgcc tgcgccgagc tggctgccag atacaacatt atttccgtcc acgtactgcc
     3181 ttgctggacg cgctttctca gtacgttgct gccgcagcag ggtacgggag aggtgatgat
     3241 tggcggtccg gtcggctttc ccggcggtgg gcataccacc gataccaagg ttcaggaagt
     3301 gcggcagctg attgccgacg gcgctcggga agtggatatg gtcgtgaata tcggcaaagt
     3361 gctctccggc gattatgact atgtgcgcga agatttgcgc cgcgtggtgg aagccgccgc
     3421 cccggtaccg gccaaagtga tccttgaaac gcactatctt aatgaggagc agattcgtcg
     3481 cgtgtgcgaa attgccgtag aggtggggat gaagtgggtc aaaacctcta ccggctgggc
     3541 gcccaccggc gccacggtgg aaaaagtcag catcatcgcc gaccagctta aaggccgaat
     3601 cgatatcaaa ggcgccgggg gcattcgcga tctggccacg gtgcgtgcgc tgtaccagct
     3661 tggcgtgcgc cgctttggca tgagccatgg cgcggtaacc aaggtgctgg ctgaactgga
     3721 gcagcacccg gaacgttttc ccgagctgaa cgccgattaa actgagagac cgcgggcctg
     3781 gtggcccgcc ttaagacgtc accggagtta gtgatgaaag ataaaatact cacgattgat
     3841 gttggcactg gcagtacccg cgccgctatt gtgcgcattg acggcgcgat gatcggcttt
     3901 gcgcaaagag agtatgagca aaccacgccg cgtgcaggtt ggtcagaaca ggccccgtct
     3961 ttatggtggc aggccgcctg cgactgtata cgcgaggtgc tttaccgcta tcctgaaacg
     4021 gcagcgcaaa ttgcggttat tggtgcttgc ggacagatgc atggcaccgt ccttctggac
     4081 gaccgtggcg agctggtgga ggatagggcg ttgctgtgga atgataagcg cagccagccg
     4141 caagttgacg cctttaacgc gcgcgaaggt tgggaaaagt ggctggcgca tttgaataat
     4201 ccgcctgcgg cagcatggcc cgctttcaag ttagcctggt ggcgcgaaaa tcatccggat
     4261 cgctggtcgc agctcgccaa agtattaatg ccgaaggact atattaattt catgctgacc
     4321 ggtgccatgg cgaccgatta tagcgaagcg tcgtgctatt tcctgatgga cagcgaaacc
     4381 cgtagctggt cttcgcaagc ctgcgaaacg tttggtctgc gggtcgatca gctcccggag
     4441 cttaaacttt ccagcgacat aatcggccag gtgacgcagc gcgcagccga cctgacgggc
     4501 ctgccggcgg gtatcccggt ggttgccggg acgtcggata tggcggcctc tctgctgggc
     4561 tcgggtgtgt atgaaccggg gatggcgtct gattcaaccg gcacgtcgac gctgatgacg
     4621 gtggtgtcgc cgcggccgtt acaccatccg ctggttaata atcttcatct tgccaacgcg
     4681 gcctggggcg gatttacgat cctcgacgcc ggcggcgatg ccgttcgctg ggcgcggctg
     4741 gcacttgccg ataaccagat tacgcatcca cagcttttac aggaagcggc ggcggtgccg
     4801 gtgggggcag aagggctgct gtttcttccc tatctgacgg gagaacgtct ggccgaacac
     4861 accaactcgc gggcacagtt ttttggcctg cagcgcaagc atcgccgcgg tcatttattc
     4921 cgcgcagttc tggaaggggt ggcctttgca tcctggcgca atcttcggca gctgcagaag
     4981 tgcgggcagt atccgcaaca aatgatcgct tccggcggcg gtgcgcgcag ctcgctgtgg
     5041 cttgaaatca aagccgcggc gtacaatctg ccgatattat ctacgcgtaa tcaggagaat
     5101 ggcgtcaccg gatgcggtat tatcgccggg gttggcgtag ggttgtacgc cgattttgcc
     5161 agcggcgtgc ggcaaacggt gcagttcgac aaactgatct caccggatcc ccgtctgcgg
     5221 gattattacc acgcctgctg cgagcttttt gatacgctgt accggcaatc cgctgcctta
     5281 tacgacaggt tggatgcgct cagcgtagga cccgactgat acgcaaaggt aagccagcgc
     5341 cgcgacgcgc gtaacgagct gtaaaaaaga gagaggcaga atgtttgcag aagaaagaca
     5401 ggagaagtta ctcggtattc tcagggaaaa acgcaaagtt gctgtttctg agatgtgtga
     5461 cgtctttaat gtatctggcg ctaccattcg tgccgatctc cgccagctgg aagaggaagg
     5521 gttgctgacc cgaacgcatg gcggagccgt tcttcgtacc cgagcgagct tcgaacaagc
     5581 atcggacgcc cgcgaaattg tcaatctcag cgcgaaagaa cggatcggcc agcgcgcggc
     5641 ggcgctggtt gaggatggcg atataatcgt gctggatacc ggtaccacga cgctgcatat
     5701 cgcgcgcaat attcgtgaca gacagaacgt cacggtggtt accaatgatt accaaatcgc
     5761 ccgggagctt gaggtcagtc cctcgattca aacgattctg ctcgggggga tcgttaagaa
     5821 gggataccac tgcgtggtgg cgatcaacgg acgttccatt ctcgacacgc taaacgtcga
     5881 taaagcattt atgggcacca attcgctgtc gctgaaacac ggcgcctgcg tggccgatat
     5941 catgctggca gaaacgaaac gcggcatggt ggaacacgcc agtcaggtga ttattgtctg
     6001 cgactatagc aagctgaata acacctcgct tgcccaattt accgcagcag atcgcattga
     6061 caccgtagtg gtggatcgtc tgcctgatga cgttcaggcg tatcgtaatg ctggcattca
     6121 ggtgatctct ctggatgaag aggagcatct ttagtgcgct gctgtgacgt tgtcactaac
     6181 cagcgcgtcg taaaaccccg tccctc
//
LOCUS       DAMZFT010000219         6203 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.219, whole genome shotgun sequence.
ACCESSION   DAMZFT010000219 DAMZFT010000000
VERSION     DAMZFT010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6203)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6203)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6203
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.219"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(<1..620)
                     /locus_tag="PN977_004875"
     CDS             complement(<1..620)
                     /locus_tag="PN977_004875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309738.3"
                     /note="role in sulfate transport across the inner
                     membrane; member of the SulP family of sulfate
                     transporters; seems to mediate transport via
                     sulfate/proton symport; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylic acid transporter DauA"
                     /protein_id="HDI6044802.1"
                     /translation="MPFRALIDACWKEKYTAARFTRDLIAGITVGIIAIPLAMALAIG
                     SGVAPQYGLYTAAVAGIVIALTGGSRFSVSGPTAAFVVILYPVSQQFGLAGLLVATLL
                     SGIFLILMGLARFGRLIEYIPVSVTLGFTSGIGITIGTMQIKDFLGLQMAHVPEHYLQ
                     KVGALFMALPTINVGDAAIGIVTLGILVFWPRLGIRLPGHLPALLAG"
     gene            complement(772..1719)
                     /gene="prs"
                     /locus_tag="PN977_004876"
     CDS             complement(772..1719)
                     /gene="prs"
                     /locus_tag="PN977_004876"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HDI6044803.1"
                     /translation="MPDMKLFAGNATPELAQRIANRLYTSLGDAAVGRFSDGEVSVQI
                     NENVRGGDIFIIQSTCAPTNDNLMELVVMVDALRRASAGRITAVIPYFGYARQDRRVR
                     SARVPITAKVVADFLSSVGVDRVLTVDLHAEQIQGFFDVPVDNVFGSPILLEDMLQLN
                     LDNPIVVSPDIGGVVRARAIAKLLNDTDMAIIDKRRPRANVSQVMHIIGDVAGRDCVL
                     VDDMIDTGGTLCKAAEALKERGAKRVFAYATHPIFSGNAANNLRNSVIDEVVVCDTIP
                     LSDEIKSLPNVRTLTLSGMLAEAIRRISNEESISAMFEH"
     gene            complement(1870..2721)
                     /gene="ispE"
                     /locus_tag="PN977_004877"
     CDS             complement(1870..2721)
                     /gene="ispE"
                     /locus_tag="PN977_004877"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HDI6044804.1"
                     /translation="MRTQWPSPAKLNLFLYITGQRADGYHTLQTLFQFLDYGDTINIE
                     LRDDGDIRLLTPVEGVEHEDNLIVRAARLLMKTAADSGRLPTGSGANISIDKRLPMGG
                     GLGGGSSNAATVLVALNHLWQCGLSMDELAEMGLTLGADVPVFVRGHAAFAEGVGEIL
                     TPVDPPEKWYLVAHPGVSIPTPVIFKDPELPRNTPKRSIETLLKCEFSNDCEVIARKR
                     FREVDAVLSWLLEYAPSRLTGTGACVFAEFDTESEARQVLEQAPEWLNGFVAKGVNLS
                     PLHRAML"
     gene            complement(2721..3344)
                     /gene="lolB"
                     /locus_tag="PN977_004878"
     CDS             complement(2721..3344)
                     /gene="lolB"
                     /locus_tag="PN977_004878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein localization protein LolB"
                     /protein_id="HDI6044805.1"
                     /translation="MPLPDFRLIRLLPLAALVLTACSVTTPKGPGKSPDSPQWRQHQQ
                     DVRNLNQYQTRGAFAYISDQQKVYARFFWQQTGQDRYRLLLTNPLGSTELELNAQPGN
                     VQLVDNKGQRYTSDDAEEMIGKLTGMPIPLNSLRQWILGLPGDATDYKLDDQYRLSEI
                     TYSQNGKNWKVVYGGYDTKTQPAMPANMELTDGGQRIKLKMDNWIVK"
     gene            3558..4814
                     /gene="hemA"
                     /locus_tag="PN977_004879"
     CDS             3558..4814
                     /gene="hemA"
                     /locus_tag="PN977_004879"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041689438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HDI6044806.1"
                     /translation="MTLLALGINHKTAPVSLRERVSFSPDKLDQALDSLLAQPMVQGG
                     VVLSTCNRTELYLSVEERDDLQEALIRWLCDYHNLNEDDLRNSLYWHQDNDAVSHLMR
                     VASGLDSLVLGEPQILGQVKKAFADSQKGHMKASELERMFQKSFSVAKRVRTETDIGA
                     SAVSVAFAACTLARQIFESLSTVTVLLVGAGETIELVARHLREHKVQKMIIANRTRER
                     AQILADEVGAEVIALSDIDERLREADIIISSTASPLPIIGKGMVERALKSRRNQPMLL
                     VDIAVPRDVEPEVGKLANAYLYSVDDLQSIISHNLAQRKAAAVEAETIVAQETSEFMA
                     WLRAQSASETIREYRSQAEHVRDELTAKALAALEQGGDAQAIMQDLAWKLTNRLIHAP
                     TKSLQQAARDGDNERLNILRDSLGLE"
     gene            4856..5938
                     /gene="prfA"
                     /locus_tag="PN977_004880"
     CDS             4856..5938
                     /gene="prfA"
                     /locus_tag="PN977_004880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012132158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HDI6044807.1"
                     /translation="MKPSIVAKLEALHERHEEVQALLGDAQTIADQERFRALSREYAQ
                     LSDVSRCFTDWQQVQEDIETAQMMLDDPEMREMAQDELREAKEKSEQLEQQLQVLLLP
                     KDPDDERNAFLEVRAGTGGDEAALFAGDLFRMYSRYAEARRWRVEIMSASEGEHGGYK
                     EIIAKISGDGVYGRLKFESGGHRVQRVPATESQGRIHTSACTVAVMPELPDAELPDIN
                     PADLRIDTFRSSGAGGQHVNTTDSAIRITHLPTGIVVECQDERSQHKNKAKALSVLGA
                     RIHAAEMAKRQQAEASTRRNLLGSGDRSDRNRTYNFPQGRVTDHRINLTLYRLDEVME
                     GKLDMLIEPIIQEHQADQLAALSEQE"
     gene            5938..>6203
                     /locus_tag="PN977_004881"
     CDS             5938..>6203
                     /locus_tag="PN977_004881"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309744.1"
                     /note="HemK; PrmC; transfers a methyl group from
                     S-adenosylmethionine to amide nitrogen of specific
                     glutamine residues in protein chain release factors PrfA
                     and PrfB; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-(glutamine-N5) methyltransferase,
                     release factor-specific"
                     /protein_id="HDI6044808.1"
                     /translation="MEYQHWLREAISQLQASESPRRDAEILLAHVTGKGRTFILAFGE
                     TQLTDEQCQQLDALLTRRRDGEPIAHLTGVREFWSLPLFVSPAT"
BASE COUNT         1564 a         1617 c         1630 g         1392 t
ORIGIN      
        1 ccagccagca aggccggaag atgaccgggt aaacgaatgc ccagacgcgg ccagaaaacc
       61 agaataccaa gcgtcacaat gccaatggct gcatcaccca cattaatggt cggcagcgcc
      121 ataaataatg cgccgacttt ttgtagataa tgttccggga catgggccat ttgcagaccg
      181 agaaaatctt taatctgcat ggtaccgatg gtgatcccga tacccgaggt gaaacctaag
      241 gtgacggaaa ccggaatata ctcaatcagg cgcccaaagc gtgccagccc catcagaatc
      301 aaaaagatcc ccgacagcaa ggtcgcaacc agcagtcctg ccagtccaaa ctgttgcgaa
      361 acgggataga gaattaccac aaatgccgca gtcggaccgg aaacgctaaa gcgtgacccc
      421 cccgtcagag caatgacaat ccccgcaaca gctgcggtat ataaaccgta ctggggtgcc
      481 acaccactac caatagccaa cgccatcgcc agcgggatag caataatccc gacggttatc
      541 ccggcaatca ggtcacgggt aaaccgtgcg gcagtatatt tttctttcca gcaagcgtcg
      601 atcagagcgc ggaaaggcat cacatgtgag gaaaatattt tgttcacaat aatgtttcat
      661 ccgtgagcgc atcatctgtc aactaaatgg caggtgaagg aggcataggt catacaaatg
      721 gatattacag acaaaaaaac ccgccgcagc gggtctttga gccgggttcg attagtgttc
      781 gaacatggca gagatcgatt cttcgttgct gatacgacga atcgcttcgg ccagcatacc
      841 tgacagggtc agagtacgca cgttcggcag tgatttgatt tcatcgctca gcggaatggt
      901 atcgcagaca acgacttcat caattacaga gttacgcagg ttgttcgccg cgttgccaga
      961 gaagatcggg tgagtcgcgt acgcaaatac acgtttagca ccacgttctt tcagagcttc
     1021 agcagcttta cacagcgtac cgccagtgtc gatcatatca tcgaccagta cgcagtcacg
     1081 acctgcaacg tcaccgatga tatgcatcac ctgggaaacg ttcgcacgcg gacgacgttt
     1141 gtcgatgatt gccatatcgg tatcgttcag cagcttagcg atagcgcggg cacgcacaac
     1201 gccgccgatg tccggagaaa ccacaatagg gttatccaga ttcagctgca gcatgtcttc
     1261 cagcaggatc gggctaccaa atacgttatc aaccggaacg tcgaagaaac cctgaatctg
     1321 ttcagcgtgc agatccactg tcagcacacg gtcaacaccg acgctggaga ggaagtctgc
     1381 aaccactttc gcagtgattg gtacacgagc ggaacggacg cgacggtcct ggcgcgcata
     1441 gccaaagtag gggataacag cggtgatacg acctgcggaa gcacgacgca gggcatcaac
     1501 cataacgact aattccatca ggttgtcgtt agtaggggca caagtggact ggatgatgaa
     1561 aatatcacca ccgcgtacat tttcattaat ttgtacgctg acttcgccgt cgctaaagcg
     1621 acctacagcg gcgtcgccga gtgaagtgta caggcggttg gcaatacgtt gtgctagttc
     1681 cggggtggcg ttaccagcaa aaagcttcat atcaggcacg agaagaacct caggcatgcg
     1741 tccattggtg gaaagaatct gccgaaaact gtgcgggcca ggcatgattc tttccaggcg
     1801 gtgtattaaa gagcgcgatg caacgtctgg aacaaggtga cgttgtcacc gaaactcagc
     1861 ttgcccggct taaagcatgg ctctgtgcaa tggggaaaga ttaacgcctt tcgccacaaa
     1921 gccattgagc cattccgggg cttgctctag cacctggcgg gcttcagact ctgtatcaaa
     1981 ttcagcaaag acacaggccc ctgtcccagt caggcgcgac ggggcgtatt ctaacagcca
     2041 ggaaagcacc gcatcaacct cgcgaaaacg ttttcttgcg ataacctcgc aatcattgct
     2101 gaattcacat tttagcaacg tttctattga cctttttggc gtattgcgcg ggagttcagg
     2161 atctttaaaa atcaccggtg tcggaatact tacaccaggg tgcgccacca gataccactt
     2221 ctctggcgga tccaccggcg ttagtatttc accaacgcct tcggcaaacg cggcatgccc
     2281 ccgaacaaag acaggaacat ctgcgcccag cgtcagtccc atttccgcca gctcatccat
     2341 gcttagcccg cattgccaga gatgatttaa tgccaccagg accgtcgcgg cattggatga
     2401 accaccgccg agaccgccgc ccatcggcaa acgcttgtca atgctgatat tcgcaccgct
     2461 tcccgtcgga agacgcccgc tgtctgccgc agttttcatc aataaccgcg ctgcgcgaac
     2521 gatcaggtta tcttcatgtt ccacgccttc aacgggcgtt aacagacgaa tatccccatc
     2581 gtcacgaagc tcaatgttga tggtgtcgcc gtaatcaaga aactgaaaca gcgtttgcag
     2641 cgtgtggtaa ccatccgcac gctgaccggt aatgtataaa aacagattaa gttttgccgg
     2701 agagggccac tgtgtccgca ttatttcact atccagttat ccatttttaa cttgatgcgt
     2761 tgaccaccgt cggtgagttc catattggct ggcatcgcag gttgagtttt tgtgtcataa
     2821 ccaccgtaaa caactttcca gtttttgcca ttctggctgt aggtgatttc gctgaggcga
     2881 tattgatcgt ccagtttgta gtcggttgca tcccccggta aacctaaaat ccactggcgc
     2941 aaactgttga gcggaatcgg cattccggtc aatttgccaa tcatctcttc ggcgtcatcg
     3001 gaggtataac gctgaccttt attgtcgact aactgcacgt tacccggttg agcattcagc
     3061 tccagttccg tgctgcccaa tgggttagtc agcagcagac ggtagcgatc ctggccagtt
     3121 tgctgccaga agaaacgggc gtacactttt tgttggtcag aaatataagc gaacgcgccg
     3181 cgagtttgat actgattaag attgcgcacg tcttgctgat gctgacgcca ttgtggcgaa
     3241 tccgggcttt tgccaggacc tttgggcgtg gtaacggaac aggcagtgag cacaagagca
     3301 gccagcggta gcaggcggat aagacgaaaa tcgggcaggg gcataataat gacaagtcct
     3361 tgagatacgt tgcagttata acccttaatg ctagcgttac cgttagccat cgtctatgtt
     3421 cacgttgtct taattgccag aatctaacgg ctttcggcaa ttactccaaa agggggcgct
     3481 ctcttttatt gatcttacgc atcctgtatg atgcaagcag actaacccta tcaacgttgg
     3541 tattatttcc cgcagacatg acccttttag cactcggtat caaccataaa acggcacctg
     3601 tatcgctgcg agaacgtgta tcgttttcgc cggataagct cgatcaggcg cttgacagcc
     3661 tgcttgcgca gccgatggtg cagggcggtg tggtgctgtc gacgtgtaac cgcacagaac
     3721 tttatctcag cgttgaagaa cgggatgacc tgcaagaggc gctcattcgc tggttgtgtg
     3781 attaccataa tctcaacgaa gacgatctgc gtaatagcct ctactggcat caggataacg
     3841 atgcggtcag tcatttaatg cgcgttgcca gtgggcttga ttctctcgtc cttggcgagc
     3901 cgcagatcct cggtcaggtt aaaaaagcgt ttgccgattc gcaaaaaggt catatgaagg
     3961 ccagcgaact ggaacgcatg tttcagaaat ctttctctgt agcgaaacgc gttcgcactg
     4021 aaacagatat cggtgccagt gctgtgtctg tcgcttttgc ggcttgtacg ctggcgcggc
     4081 agatctttga atcgctctct acggtgacgg tactgctggt gggtgcgggc gaaaccatcg
     4141 agctggtggc gcgtcatctg cgtgaacaca aagtacagaa gatgattatc gccaaccgca
     4201 ctcgcgaacg tgcccaaatt ctggcagatg aagtcggcgc ggaagtgatt gccctgagcg
     4261 atatcgacga gcgtctgcgc gaagccgata tcatcatcag ttccaccgcc agcccgttac
     4321 cgattatcgg taaaggcatg gtggagcggg cattaaaaag ccgtcgcaac caaccaatgc
     4381 tgttggtgga tattgccgtt ccgcgcgatg ttgagccgga agttggcaaa ctggcgaatg
     4441 cttatcttta cagcgttgat gatctgcaaa gcatcatttc gcacaacctg gcgcagcgta
     4501 aagccgcagc ggttgaggcg gaaactattg tcgctcagga aaccagcgaa tttatggcgt
     4561 ggctgcgcgc acaaagcgcc agcgagacca ttcgcgagta tcgcagccag gcagagcacg
     4621 ttcgcgatga gttaaccgcc aaagcgttag cggcccttga gcagggcggc gacgctcaag
     4681 ccattatgca ggatctggca tggaaactga ctaaccgcct gatccatgcg ccaacgaaat
     4741 cacttcaaca ggccgcccgt gacggggata acgaacgcct gaatattctg cgcgacagcc
     4801 tcgggctgga gtagcagtac atcattttcc ttttttacag ggtgcattta cgcctatgaa
     4861 gccttctatc gttgccaaac tggaagccct gcatgaacgc catgaagaag ttcaggcgtt
     4921 gctgggtgac gcgcaaacta tcgccgacca ggaacgtttt cgcgcattat cacgcgaata
     4981 tgcgcagtta agtgatgttt cgcgctgttt taccgactgg caacaggttc aggaagatat
     5041 cgaaaccgca cagatgatgc tcgacgatcc tgaaatgcgt gagatggcgc aggatgaact
     5101 gcgcgaagct aaagaaaaaa gcgagcaact ggaacagcaa ttacaggttc tgttactacc
     5161 aaaagatcct gatgacgaac gtaacgcctt cctcgaagtc cgtgccggaa ccggcggcga
     5221 cgaagcggcg ctgttcgctg gcgatctgtt ccgtatgtac agccgttacg ccgaagcccg
     5281 ccgctggcgt gtagaaatca tgagcgccag cgagggtgaa catggtggtt ataaagagat
     5341 catcgccaaa attagcggtg atggtgtata tggtcgtttg aaattcgaat ctggcggtca
     5401 tcgcgtgcag cgtgttcctg ctacggaatc gcagggtcgt attcatactt ctgcttgtac
     5461 cgttgcggta atgccagaac tgcctgacgc agaactgccg gacatcaacc cagcagattt
     5521 gcgcattgat actttccgct cgtcaggggc gggggggcag cacgttaaca ccaccgattc
     5581 ggcaattcgt attactcact tgccgaccgg aattgttgtt gaatgtcagg acgaacgttc
     5641 acaacataaa aacaaagcta aagcactttc tgtactcggt gctcgtatcc acgctgctga
     5701 aatggcaaaa cggcaacagg ccgaagcgtc tacccgtcgt aacctgctgg ggagtggcga
     5761 tcgcagcgac cgtaaccgta cttacaactt cccgcagggg cgcgttaccg atcaccgcat
     5821 caacctgacg ctctaccgcc tggatgaagt gatggaaggt aagctggata tgctaattga
     5881 accgattatc caggaacatc aggccgacca actggcggcg ttgtccgagc aggaataatg
     5941 gaatatcaac actggttacg tgaagcaata agccaacttc aggcgagcga aagcccgcgg
     6001 cgtgatgctg aaatcctgct ggcgcatgtt accggcaaag ggcgtacttt tattctcgcc
     6061 tttggtgaaa cgcagctgac tgacgaacaa tgtcagcaac ttgatgcgct actgacgcgt
     6121 cgtcgcgatg gtgaacctat tgctcattta accggggtgc gagaattctg gtcgctgccg
     6181 ttatttgttt cgccagcgac ctt
//
LOCUS       DAMZFT010000220         6146 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.220, whole genome shotgun sequence.
ACCESSION   DAMZFT010000220 DAMZFT010000000
VERSION     DAMZFT010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6146)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6146)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6146
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.220"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(<1..1250)
                     /locus_tag="PN977_004882"
     CDS             complement(<1..1250)
                     /locus_tag="PN977_004882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013124600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDI6044809.1"
                     /translation="MATDTPRIPEQGVATLPDEAWERARRRAEIISPLAQSETVGHEA
                     ADMAAQALGLSRRQVYVLIRRARQGSGLVTDLVPGQSGGGKGKGRLPEPVERVIHELL
                     QKRFLTKQKRSLAAFHREVTQVCKAQKLRVPARNTVALRIASLDPRKVIRRREGQDAA
                     RDLQGVGGEPPAVTAPLEQVQIDHTVIDLIVVDDRDRQPIGRPYLTLAIDVFTRCVLG
                     MVVTLEAPSAVSVGLCLVHVACDKRPWLEGLNVEMDWQMSGKPLLLYLDNAAEFKSEA
                     LRRGCEQHGIRLDYRPLGQPHYGGIVERIIGTAMQMIHDELPGTTFSNPDQRGDYDSE
                     NKAALTLRELERWLTLAVGTYHGSVHNGLLQPPAARWAEAVARVGVPAVVTRATSFLV
                     DFLPILRRTLTRTGFVIDHIHYYA"
     gene            1523..1696
                     /locus_tag="PN977_004883"
     CDS             1523..1696
                     /locus_tag="PN977_004883"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6044810.1"
                     /translation="MSFLALFVSLPTKASTGRMRVWRSVKAQGCATLRDGVYLLPDSA
                     DSAATLGEVVGGK"
     gene            complement(2309..3680)
                     /locus_tag="PN977_004884"
                     /pseudo
     CDS             complement(2309..3680)
                     /locus_tag="PN977_004884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000971921.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182-like element ISCfr1 family transposase"
     gene            complement(3795..4931)
                     /locus_tag="PN977_004885"
     CDS             complement(3795..4931)
                     /locus_tag="PN977_004885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3883 domain-containing protein"
                     /protein_id="HDI6044811.1"
                     /translation="MSQLSQLRSPAAVQAAIDEFVQLGRTKFLARHGYGKSRDFLVRD
                     PKTGTDCDSKAIAGVAFGKQFPEQGPLTADSFSGGEATVVPALTRLGFRIIRIGEDWS
                     EEEVLATVEDYFDMLRAEAAGEPYNKSEHNQALRQLLNGRSKSSVELKHQNISAVLDA
                     LGLPYINGYKPRGNSQLLLRKSVHAYVLEHQQTVGALVDALEEVKLPGDKTYRAALVE
                     PPAREVLVRTPASLRQRLPRKFDYAARDEANRKLGRAGEQWVIGYEQQRLTELGHPEL
                     FQRLDWVSDTQGDGAGFDILSFEEDAHERFIEVKTTNGGVGSSFLVSHNELEFSKEAG
                     DQFHLYRVFQFRDGPRLFTLPGDLSQHVHLKPTDYRASFRSLVG"
     gene            complement(4982..5209)
                     /locus_tag="PN977_004886"
     CDS             complement(4982..5209)
                     /locus_tag="PN977_004886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6044812.1"
                     /translation="MVNSYSLSDDSGVMAAAAITHFLFGQAVFSYLNGWSVLIGPGTG
                     LDSTGCKYARDLMGLVAFTAFIVTFLFRGYS"
     gene            5233..5424
                     /locus_tag="PN977_004887"
     CDS             5233..5424
                     /locus_tag="PN977_004887"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6044813.1"
                     /translation="MAIALMGAGFSATDTSDAVNILYPITMTVQANKAWQASGLKKSF
                     FLPSHKKVRGGEKISYARL"
     gene            complement(5905..>6146)
                     /locus_tag="PN977_004888"
     CDS             complement(5905..>6146)
                     /locus_tag="PN977_004888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tunicamycin resistance protein"
                     /protein_id="HDI6044814.1"
                     /translation="LLHIFLTLNEDLLRHRIANQTMHPDPNRNAEIREWRLANVARCL
                     AARERLPCTTRVLDSGAHTSDELAAMVLDGIDGRT"
BASE COUNT         1274 a         1791 c         1806 g         1275 t
ORIGIN      
        1 tcggcgtagt agtggatgtg gtcgatgaca aagccggtgc gggtcagcgt gcgccggagg
       61 atcggcagaa aatcgaccag gaacgaagta gcgcgtgtga cgacggccgg tacgccgaca
      121 cgcgccacgg cctcggccca gcgcgcggcc ggcggttgga gcaggccgtt gtgcaccgaa
      181 ccgtggtagg tgccgaccgc caatgtgagc cagcgctcta gctcgcgcag cgtcagggcg
      241 gccttgtttt cggaatcgta gtcgccgcgc tggtcagggt tggagaaggt cgttcccggc
      301 agttcgtcgt gaatcatctg catcgccgtg ccgatgatcc gttccacgat gccgccatag
      361 tgcggctgtc ccagcgggcg atagtccagc cggatgccat gctgctcgca accccggcgc
      421 agggcctcgc tcttgaactc ggccgcgttg tctaggtaga gcagcaaggg cttgccgctc
      481 atctgccaat ccatttccac gttcagtcct tccagccaag ggcgcttgtc gcaggcgaca
      541 tgcacgaggc acaggccaac cgaaacggca gacggcgctt ccagcgtgac gaccatgccg
      601 agcacgcagc gggtgaacac gtcgatggcg agggtcaggt acgggcggcc aataggttgc
      661 cggtcgcggt catcgaccac gatcaggtcg atgaccgtat ggtctatctg cacctgctcc
      721 agcggcgcgg tcacggcagg aggctcgccg cccacacctt gtaggtcacg agcggcatcc
      781 tggccttccc gccggcggat gaccttgcgc gggtcaaggc tagcgatccg taaggccacg
      841 gtattgcgcg ccggcactcg cagtttttga gccttgcaca cctgagtgac ttcgcggtga
      901 aaggccgcta ggctgcgctt ctgcttggtc aggaaccgct tttgcagtag ctcgtggatg
      961 acgcgctcga ccggttccgg caagcgcccc ttacctttac ctccaccgga ctggccgggc
     1021 accagatccg tcacgaggcc gctgccttgc cgggcacgcc ggatcagaac gtatacctgg
     1081 cgccgagaca agcccagcgc ctgagccgcc atatcggccg cttcgtgccc gaccgtctcc
     1141 gactgcgcca acggactgat gatctccgca cgacggcgcg cacgctccca agcctcatca
     1201 ggcagagtgg ccacgccttg ttctggaatc cgtggggtgt ccgtcgccat gctcacctcg
     1261 ctttggtgca cacgagtatt gagcatagtc gagattggtg cagatcactt ctgatattga
     1321 actgtcagga gctggctgca caacagccat tacgcccaat caactggtgc agtcgtcttc
     1381 tgaaaatgac acgcagcgtg gccattcctg acatccagtt agggtatgcc ttaaattgac
     1441 gctccgacat gagttgtgta ttagatataa cttattgtag tatttgtaac attaatcatc
     1501 tttttgacga aggagcccgt gcttgagctt tcttgcccta ttcgtcagcc tgcccaccaa
     1561 ggcatcgacc ggccgcatgc gcgtctggcg ttctgtcaaa gcgcagggct gcgcaacgct
     1621 gcgcgacggg gtctatctgc tgcccgactc ggcggacagt gcggcgacgc tgggcgaagt
     1681 tgtgggagga aaataaagtg tcatcctaac tacaggaccc tgcaacgtag caattcctgc
     1741 aaaaatccat ttaaaacaat gacttatttc aattctgcca tctgcaagcg ttttgtgggg
     1801 atggaggccg gaatcattaa agtatcatcc taattttcgc tcaaccttgg aaaatgctga
     1861 gaatcgtaaa aataaagttt catcctttcc gctactagtt caaggctttc ttccggccgc
     1921 cggcaacaac gctgcttggc tgaggcgatc cacttgcgct accgctcact gatcagatcg
     1981 aacttgaact cgacgagcag ccaaccatca ggccgagcgt gatcgccaaa aaaaacggat
     2041 catgcgaaat ccttcgttga gtttggcgcg ggttttagca aatcagctgg cccgcgatcc
     2101 ggttgagagt gatccgaagc ggtccttgac gaccggcaca aatcggccga ttctgttgaa
     2161 aaagtagctt tagcggcagc ctgccgatca ggtgtgcctg ctgtcgaagt ggctgcaagc
     2221 cacttcaagt tgccttccgg cgtttcactg agcgtccttg ctcaggttta agggttaatt
     2281 tgagggtttc tgctcgtagc aggcatacct atcccgtcag cggtggccct tgaggcaaaa
     2341 gcttggccat gcggcgcagg ttctgcacca tcgcagccaa ggtgaattcg tcagtggcac
     2401 ccgttaggcc acgcagtcgt aaacggtcga gtttcatgat ccgtttgagg tgggcgaaaa
     2461 gcatctccac cttctttcgt tcgcagcgag agacgaggta ctccggtgtc ttggcgatgc
     2521 gtcgagccac gtcgcgggca gcctcatgga tgctgcggac gatcttccga ttcggcgtgt
     2581 tggggcagca tttcgctttc aacgggcagg tggcgcagtc ggtttggctg gagcggtaaa
     2641 tgacggtttt ggccttagtt acccgcgacc tttgctgggt gaaggcgcgc cattcactgc
     2701 gtagcggttt gccggctggg cagcgatatt cattggcgtc ctgactccag tgaaagtcgt
     2761 tactggagag gctgtcgtcc ttgcgctcgg tcttgtccca caccggcaca tgcggttcga
     2821 tgtccttttc ttcgaccatc caggccagca tcggggcggt gccataagcg gtatcgccga
     2881 taaggcgttc cggtgtgaga tcgaactgcg cctcgacacg ctcgaccatc gtcctagtcg
     2941 aatcgacttc ggcggtacgg tgcgccgggg tagcttccac gtccatgatc acaccgtgct
     3001 cagtgtcgat caggtaattc gtggagtagg caaaaaaggc cgggccacct ggcgctgctg
     3061 tccaacggga ctgaggatca gtgagcgaaa ttttcttggg aagagcctca gccagcgcct
     3121 cttcatcaag ggcttcgagg tactcgcgca ctgcgcggct gctgagcttt ggatcgttcc
     3181 aatcgacctc atctcccgcc accccacgtt gccggctggc atccgcctta atgatgctgg
     3241 cgtcgacggc gaaaccttca cccttgacta ggccggctgc catgcagcgc cgcagcacct
     3301 cattgaataa ccagcggaat agatcgctgt cacgaaaacg cccatggcga ttcttcgaga
     3361 aggtcgagtg attggggact tcgtcttcca gacccaaccg gcagaaccag cgataggcca
     3421 ggttcaggtg cacctcttcg cacaatcgcc gctcggaacg aatgccatag caagtagccg
     3481 acgaccagca tgcgcaccat caactccggg tcaatcgagg gacgcccgat ggggctatag
     3541 aaatctgcca ggtaggcacg tagatcactg agatccaagc actggtcgat gctgcgcagg
     3601 agatgttggg ccgggacgtg atcttccaga ttgaacgagt agaacaggcg ctgctgtcct
     3661 cccggtaact gtcccatcat gctgttcgcc cccacgctcg ctgacaaagc aattttgcca
     3721 acggcatggg gaggccgcta ctttttcaac agaatcggcc gcacacagcc attcggctca
     3781 acagaaccct gcctttaccc caccaaactc cggaaactcg cccggtagtc cgtcggcttg
     3841 agatgcacat gttggctgag gtcgccgggt agcgtgaaca ggcgcggacc gtcccgaaac
     3901 tggaacacgc gatacagatg gaattgatcg cccgcctcct tggagaattc gagttcgttg
     3961 tggctgacca agaaagacga gcctaccccg ccattggtgg ttttcacctc gatgaagcgc
     4021 tcatgggcgt cctcttcgaa cgacaggatg tcgaaccccg caccgtctcc ctgggtgtcg
     4081 gacacccaat ccagccgctg aaaaagctct gggtggccga gctcggtcag gcgttgctgt
     4141 tcgtagccaa tcacccactg ctcccctgcc cggcccagct tgcggttggc ttcatcgcga
     4201 gcggcataat cgaactttcg cggtaggcgt tgccgtagag atgccggggt acgcacaagc
     4261 acttcacggg cgggtggttc taccaaagcc gctcggtagg ttttgtcacc cggaagtttt
     4321 acctcctcca gggcatcgac aagagcgccg accgtctgct gatgttccag aacgtaggcg
     4381 tgtacggatt tacgcagcag cagttggctg ttgccgcgtg gcttgtagcc gttgatatag
     4441 ggcaggccca gggcatcgag tacggcgcta atgttctggt gcttgagctc gactgaagac
     4501 ttgctgcgac cgttcagcag ttggcgcagt gcctggttgt gctcggactt gttgtacggc
     4561 tccccagccg cctcggcacg cagcatgtcg aaatagtctt cgaccgtggc caggacctct
     4621 tcttcggacc agtcttcgcc gatgcgaatg atgcgaaacc cgagccgcgt cagcgccgga
     4681 acgacggtcg cctcgccacc ggagaagctg tcagcagtga gcgggccctg ctcgggaaat
     4741 tgcttgccga aggccacacc ggcgatggcc ttggaatcgc aatcggtgcc ggtcttcgga
     4801 tcacgtacca ggaagtcgcg ggacttgccg tagccgtggc gcgccaggaa tttcgtgcgg
     4861 cccagttgca cgaactcatc gatggcagcc tgcacggcgg cggggcttcg aagctgggag
     4921 agttgagaca cagggtcctt ccttactgtc atggtgtgcc gggaaccgcc gagccacgag
     4981 attatgagta gcccctgaac agaaacgtca cgataaaagc cgtgaacgcc accaggccca
     5041 ttaaatccct tgcgtatttg cagcccgtgc tgtccaaacc tgtaccaggt ccgatcaaca
     5101 cgctccaacc attgaggtac gaaaacaccg cctgaccgaa caaaaaatgc gtgatagccg
     5161 ccgcagccat gacgccggaa tcgtcagaca ggctgtagct gttgaccatt gccctgtacg
     5221 cctgcatctg aaatggctat tgccctgatg ggagccggct tttcagccac cgacaccagc
     5281 gatgccgtca atattcttta cccgataacc atgaccgtac aagctaacaa ggcctggcaa
     5341 gccagcggac ttaaaaagtc tttttttcta ccatcccaca aaaaagttcg tggtggagaa
     5401 aagataagct atgcaaggct ttaggagacg tggtttttca ggatgacgaa gaacgattcg
     5461 gcgctaggtg caacataggt gcatcgcacg agcgctagga acggcgaaaa aaggcggacg
     5521 tggcgaaatc ggtagacgca gcagacttta aaattggagt gcccgcgggg aaatccgcgg
     5581 agtagaaccg ctcaaagtcg gggaacgcta acgggcaata ccctaagcca atcccgagcc
     5641 aagccccttc gggggaaggt gtagagactg gacgggcggc gcctaaagcc ttcgggcaat
     5701 ggcgaaggga cagtccagac cacgaacgtc atcagacggc ggcgaaagtc gaggtggtac
     5761 gaaaatctgc ttctctgtga gagtacgggt tcgagtcccg tcgtcgcacc acaaagccaa
     5821 acatccctgc gatgatcgac ctctgggcgt ttggtcgtga gcccgccacc ctcgcgctac
     5881 gctttgcgca ggcgtcgaag gcgatcaggt gcgcccatcg attccgtcga gcaccatcgc
     5941 tgcgagttca tcgctggtgt gtgcaccact atcgagaaca cgggttgtgc atggaagccg
     6001 ttcccttgcg gccaagcatc gggcgacatt cgctaatcgc cactctcgaa tctccgcatt
     6061 tcgattcggg tcaggatgca tggtctggtt cgcgatccgg tgacgcaata ggtcctcgtt
     6121 gagcgtcaga aagatgtgca gcagct
//
LOCUS       DAMZFT010000221         6136 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.221, whole genome shotgun sequence.
ACCESSION   DAMZFT010000221 DAMZFT010000000
VERSION     DAMZFT010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6136)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6136)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6136
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.221"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(90..617)
                     /gene="mobB"
                     /locus_tag="PN977_004889"
     CDS             complement(90..617)
                     /gene="mobB"
                     /locus_tag="PN977_004889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312806.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein B"
                     /protein_id="HDI6044815.1"
                     /translation="MAGKTMIPLLAFAAWSGTGKTTLLKKLIPALCARGIRPGLIKHT
                     HHDMDVDKPGKDSYELRKAGAAQTIVASQQRWALMTETPGEEELDLHFLASRMDTSKL
                     DLILVEGFKHEEIAKIVLFRDGAGHRPEELVIDRHVIAVASDVPLNLDVALLDINDVG
                     GLADFVVEWMQNQDG"
     gene            complement(599..1183)
                     /gene="mobA"
                     /locus_tag="PN977_004890"
     CDS             complement(599..1183)
                     /gene="mobA"
                     /locus_tag="PN977_004890"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase MobA"
                     /protein_id="HDI6044816.1"
                     /translation="MNLMTTITGVVLAGGKARRMGGVDKGLLELNGKPLWQHVADALM
                     TQLSHVVVNANRHQEIYQASGLKVIEDSLADYPGPLAGMLSVMQQEAGEWFLFCPCDT
                     PYIPHDLASRLTHQRKDAPVVWVHDGERDHPTIALVNRAIEPLLQEYLQAGERRVMAF
                     MRLAGGHAVDFSDRKEAFINVNTPEELARWQEKR"
     gene            1253..1522
                     /locus_tag="PN977_004891"
     CDS             1253..1522
                     /locus_tag="PN977_004891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihD family protein"
                     /protein_id="HDI6044817.1"
                     /translation="MKCKRLNEVIELLQPAWQKEPDLNLLQFLQKLAKESGFDGELAD
                     LTDDILIYHLKMRDSAKDAVIPGLQKDYEEDFKTALLRARGVIKE"
     gene            1599..2585
                     /gene="srkA"
                     /locus_tag="PN977_004892"
     CDS             1599..2585
                     /gene="srkA"
                     /locus_tag="PN977_004892"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response kinase SrkA"
                     /protein_id="HDI6044818.1"
                     /translation="MNNSAFTFQTLHPDTIMDALFEQGIRVDSGLTPLNSYENRVYQF
                     QDEDRRRFVVKFYRPERWAADQILEEHQFALQLVNDEVPVAAPVAFNGQTLLNHQGFY
                     FAVFPSVGGRQFEADNIDQMEAVGRYLGRMHQTGRKQLFIHRPTIGLNEYLIEPRKLF
                     EDATLIPSGLKAAFLKATDELIAAVTAHWREDFTVLRLHGDCHAGNILWRDGPMFVDL
                     DDARNGPAIQDLWMLLNGDKAEQRMQLETIIEAYEEFSEFDTAEIGLIEPLRAMRLVY
                     YLAWLMRRWADPAFPKNFPWLTGEDYWLRQTATFIEQAKVLQEPPLQLTPMY"
     gene            2602..3228
                     /gene="dsbA"
                     /locus_tag="PN977_004893"
     CDS             2602..3228
                     /gene="dsbA"
                     /locus_tag="PN977_004893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA"
                     /protein_id="HDI6044819.1"
                     /translation="MKKIWLALAGLVLAFSASAAQYEDGKQYTTLEKPVAGAPQVLEF
                     FSFFCPHCYQFEEVLHISDNVKKKLPEGVKMTKYHVNFMGGDLGKDLTQAWAVAMALG
                     VEDKVTVPLFEGVQKTQTIRSASDIRDVFINAGIKGEEYDAAWNSFVVKSLVAQQEKA
                     AADVQLRGVPAMFVNGKYQLNPQGMDTSNMEVFVQQYADTVKYLSEKK"
     gene            3383..4812
                     /locus_tag="PN977_004894"
                     /pseudo
     CDS             3383..4812
                     /locus_tag="PN977_004894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418298.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
     gene            complement(4853..5785)
                     /locus_tag="PN977_004895"
     CDS             complement(4853..5785)
                     /locus_tag="PN977_004895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HDI6044820.1"
                     /translation="MANLLNKFIMTRILAAITLLLSIVLTILVTIFCSVPIIIAGIVK
                     LLLPVPVIWRKVSRFCDFMMYCWCEGLAVLLHLNPHLQWEVHGLEGLSKKNWYLLICN
                     HRSWADIVVLCVLFRKHIPMNKYFLKQQLAWVPFLGLACWALDMPFMKRYSRAYLLRH
                     PERRGKDVETTRRSCEKFRLHPTTIVNFVEGSRFTQEKHQQTHSTFQNLLPPKAAGIA
                     MALNVLGKQFDKLLNVTLCYPDNNRQPFFDMLSGKLTRIVVHVDLQPISDELHGDYIN
                     DKSFKRHFQQWLNSLWQEKDRLLTSLMSSQRQDK"
BASE COUNT         1740 a         1368 c         1410 g         1618 t
ORIGIN      
        1 aaaggccatc caaaaggatg gcctttttgc gctggtgcag aaaaaggccg gctgcgacgc
       61 tggctgcgtc tgatccagcc tactcttgct tatccgtcct gattttgcat ccactccacc
      121 acaaaatcag ccagcccccc aacatcatta atatccagta acgcgacatc aagattaagc
      181 ggcacatcac tggctacagc aataacatgc ctgtctatca ctaattcttc cggtcgatgt
      241 ccggctccat cgcgaaacag cacaatcttt gcgatctctt catgcttaaa cccttcgacc
      301 agaatcaaat ccagctttga ggtatccatt cgacttgcga gaaaatgtag atccagctct
      361 tcttcgcctg gtgtttccgt catcaaggcc catcgttgct ggctggcaac gatggtttgt
      421 gccgcgccag ccttgcgcag ctcatagcta tctttgcctg gcttatcaac atccatatca
      481 tggtgcgtat gcttaatcag ccccggacgg atccctctgg cgcataatgc cgggatcaat
      541 tttttcagca gcgttgtttt tccggtgcca ctccatgcag caaaggcgag taacggtatc
      601 atcgtttttc ctgccatcgg gcaagctcct ctggcgtatt cacgtttata aatgcctctt
      661 tacgatcgct gaaatcaacc gcatgaccgc cagccagacg cataaatgcc ataacccggc
      721 gttctcctgc ttgcagatat tcttgtaata aaggctcaat tgcgcggttt accagagcaa
      781 tagtcgggtg atcgcgttca ccgtcgtgga cccacacgac aggcgcatct ttgcgctgat
      841 gagtaagccg ggaggctaaa tcatggggaa tgtaaggcgt atcgcacggg caaaacaaaa
      901 accactcacc cgcttcctgc tgcattactg aaagcattcc tgccagaggg ccagggtaat
      961 ccgccagtga atcttcaatc actttcagac cgcttgcctg atagatttcc tgatgacgat
     1021 tagcattaac cacgacgtga gagagctgcg tcataagcgc gtcagcgaca tgttgccata
     1081 atggtttgcc gtttaattca agcaatcctt tatctacgcc gcccattcgt ctggctttac
     1141 cgcccgccag cacaacgcct gttatcgtcg tcatcagatt caccgatatc gcctctttta
     1201 ttgtgggatt gaccctgcta acgtgtctgt ctcaagagta aggagcatta ctatgaaatg
     1261 taaacgtctg aatgaagtta ttgaactcct ccagccagcc tggcaaaaag agccagacct
     1321 taacctgctg caatttttgc agaaactggc gaaagagtca ggttttgacg gcgaactggc
     1381 ggatttgacg gatgacattc tgatctatca cctgaaaatg cgtgattccg cgaaagatgc
     1441 ggtgatcccg ggtttgcaga aagattatga agaagatttc aaaacggcgc tattacgcgc
     1501 acgtggtgta attaaagagt aaaagcttgt aagcggtgcc accaaaatca tcatgaaatg
     1561 atatccttca tcattcgtaa tgttttccgg atgatgggat gaacaacagc gcttttactt
     1621 tccagacact acacccggat accatcatgg atgctctgtt tgagcaaggg atccgggtgg
     1681 attccggtct taccccgctt aacagctatg aaaaccgtgt ctatcaattt caggacgaag
     1741 atcgtcgacg ttttgtcgtc aaattttatc gtcctgaacg ttgggcagcc gatcaaatcc
     1801 tcgaagaaca tcaatttgcg ttgcagttgg taaatgatga agttccggtc gcagcacctg
     1861 tggcctttaa cggtcagact ttattgaatc atcagggatt ttatttcgct gtttttccaa
     1921 gcgtcggtgg tcgccagttc gaagctgata atatcgatca gatggaagcg gttgggcgtt
     1981 atttagggcg tatgcaccag acggggcgca aacagctttt tatccatcgc ccgaccatcg
     2041 gtctgaatga atatctcatt gagcctcgca agctgtttga ggacgctaca ctgatacctt
     2101 ccgggttgaa agcggcattt ctgaaagcga cagatgagct gatcgccgcc gttacagcac
     2161 actggcggga agatttcacc gttctgcggc tacatggaga ctgccacgct gggaatattc
     2221 tctggcgcga tggtccaatg tttgttgatc tggatgatgc acgtaatggt ccggctattc
     2281 aggatttgtg gatgttgctc aatggcgata aagccgagca gcggatgcaa ctggaaacta
     2341 ttattgaggc ttatgaagaa tttagcgagt tcgacaccgc tgaaatcgga ctgattgaac
     2401 ctttacgcgc catgcgtttg gtttattatc ttgcctggct aatgcggcgt tgggctgatc
     2461 ccgcgttccc gaaaaatttc ccgtggttaa ccggggaaga ttactggcta cgacagacgg
     2521 cgacttttat agaacaggca aaagttctac aagaaccccc tttgcaatta acacctatgt
     2581 attaatcgga gagagtagat catgaaaaag atttggctgg cgctggctgg tttagtttta
     2641 gcgtttagcg catcggcggc gcagtatgaa gatggtaaac agtacactac cctggaaaaa
     2701 ccggtagctg gcgcgccgca agtgctggag tttttctctt tcttctgccc gcactgctat
     2761 cagtttgaag aagttctgca tatttctgat aacgtgaaga aaaaactgcc ggaaggcgtg
     2821 aagatgacta aataccacgt caacttcatg ggtggtgacc tgggcaaaga tctgactcag
     2881 gcatgggcag tggcgatggc actgggcgtg gaagacaaag tcactgttcc gctgtttgaa
     2941 ggcgtacaga aaacccagac cattcgttca gcatctgata tccgcgatgt atttatcaac
     3001 gcaggtatta aaggtgaaga gtacgacgcg gcgtggaaca gcttcgtggt gaaatccctg
     3061 gtcgctcagc aggaaaaagc tgcagctgac gtgcaattgc gtggcgttcc ggcgatgttt
     3121 gttaacggta aatatcagct gaatccgcag ggtatggata ccagcaatat ggaagttttt
     3181 gttcagcagt atgctgatac ggtgaaatat ctgtccgaga aaaaataatt caatgtaaat
     3241 tacataaagc ccgtgaatat tcacgggctt tttttattat ttaataaata taaatacatt
     3301 ctgataatgc gtcctgccgc tggacattat ttcagcataa gtggatattc aaagttttgc
     3361 tgttttatca gggaatattt atatgatacg taagtcagct acaggtgtta ttgttgcgtt
     3421 agccgtaatc tggggtggtg gcacatggta cacaggtacg caaattcagc ctggtgtcga
     3481 aaagtttatt aaagatttta acgatgctaa aaaaaaggtg aacatgccta cgatatgacg
     3541 ttaagttata aaaactttga caaaggcttt tttaattctc attttcaaat gcaaataaca
     3601 tttgataacg gtgcacccga tctcaatatc aagccaggcc agaaagttgc atttgatgtg
     3661 gatgttgagc acggtccgtt gcccatcaca atgttaatgc atggtaatgt catcccggca
     3721 ctggcagcgg caaaagtgaa cttagtgaat aatgaactga cacaaccgct atttatcgcc
     3781 gcgaaaaata aatcgcccgt ggaagcgaca ttgaggttcg cgtttggtgg ctcattctcc
     3841 acgacattag atgttgcccc tgcagagtat ggaaagtttt cctttggtaa gggccagttt
     3901 acttttaatg gtgatggtag ttcattgtct aacctggata ttgaaggcaa agtcgaagat
     3961 attgttctgc aattttcacc aatgaacaaa gtaacggcga aaatttttac ctttgattct
     4021 ctgacacgat tagaagaaaa gaaatttccg gttggtgaaa gcgagtcgaa atttaatcag
     4081 gttaatatta tcaaccaggg ggaagtcgtt gcccaaatcg atgctttcgt tgcaaaaacc
     4141 aggctggatc gcgttaaaga caaagattat atcaatgtca atctgaccta cgaacttgat
     4201 aagttaataa aagggaatca gcaactcggt agtggtgagt ggtcattgat tgctgaatct
     4261 attgatccct cagcggtgcg ccaatttatc atccagtata acattgcgat gcagaagcag
     4321 cttgctgcac accctgagtt agcaaacgat gaagttgctc tgcaagaagt gaatgctgca
     4381 ttgttcaaag agtatttacc gttattacaa aaaagtgagc cgaccattaa acaaccggta
     4441 agatggaaga acgcactcgg cgaactaaat gccaatctgg atatcagtat tgccgacccc
     4501 gccaaatctt catcatccac aaacaaagat attaaatcgc tcaattttga tgtgaagtta
     4561 ccgcttaatg tcgtcacaga aaccgcaaaa cagcttaatt tatctgaagg aatggatgcg
     4621 gaaaaagcgc aaaagcaggc tgataaacaa atcagcggga tgatgacatt aggtcagatg
     4681 tttcagttaa tcacgattga caacaatacc gcctcgctgc agctgcgtta tacaccgggt
     4741 aaagttgttt ttaacggaca ggagatgagc gaagaagaat ttatgtctcg tgccggacgt
     4801 tttgttcatt aaaatactca ctcgcctctg ctcattcaga ggcgagtaaa acttacttgt
     4861 cctgccgctg tgatgacatc aacgacgtca gaagccggtc tttttcctgc cacagtgagt
     4921 tcagccattg ctgaaaatgg cgcttaaagc ttttgtcatt gatgtagtcg ccatgtaact
     4981 catcggaaat aggctgcaaa tccacatgta cgacaattcg cgtcagttta ccgcttaaca
     5041 tatcgaagaa cggctgacgg ttattgtccg gataacacag cgtaacatta agcagtttat
     5101 cgaattgttt acccagtaca tttagcgcca tcgcaatgcc tgccgccttt ggtggcaaca
     5161 agttttgaaa agtggagtgg gtttgctgat gtttttcttg cgtgaaacgg gagccttcaa
     5221 cgaaattgac aatggtggtg ggatgcaggc gaaacttttc acaagaacgc cgagtggttt
     5281 caacatcttt accacgtcgc tccggatgac gtaataaata agcgcgggaa taacgcttca
     5341 taaacggcat atccagcgcc cagcacgcca ggccaagaaa tggcacccag gccagctgtt
     5401 gcttgaggaa atatttattc atcggaatgt gcttacgaaa cagcacgcac agtacgacaa
     5461 tatctgccca actacggtgg ttacaaataa gcagatacca gttcttctta cttaaccctt
     5521 ccagcccgtg aacttcccat tgcaggtgtg ggttcagatg cagtaatacc gccagacctt
     5581 cacaccagca atacatcata aaatcacaaa aacgcgaaac ctttcgccaa atgactggca
     5641 caggtagcaa tagttttaca atcccggcaa tgatgatcgg gacggaacaa aaaatagtga
     5701 ccaaaatggt caatacgata ctcagcaaaa gggttatcgc agcgagtatt ctcgtcataa
     5761 tgaatttatt caaaaggtta gccatagaca gtgcgctaaa gaagcgcaag gcgcagattt
     5821 tagcagaaaa cgacccaaat aacggatgat ccttaaggag aaaaataatt catatctatc
     5881 cacattagaa aaaatcccat tatctcaatt attagggatg gctttatttt taactgcatg
     5941 aaaaacaaag ataaacatca tgctgtaaaa agcatgataa taaattaaaa gtgatgtaaa
     6001 taatttatgc acaaagttat ccacatgacg atttgcgagc gatccagaag atctacaaaa
     6061 gattttcacg aaaagcggtg aaaaactcat gttttcatcc tgtctgtggc atcctttacc
     6121 cataatctga taaaca
//
LOCUS       DAMZFT010000222         6012 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.222, whole genome shotgun sequence.
ACCESSION   DAMZFT010000222 DAMZFT010000000
VERSION     DAMZFT010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6012)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6012)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6012
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.222"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..250
                     /locus_tag="PN977_004896"
     CDS             <1..250
                     /locus_tag="PN977_004896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDI6044821.1"
                     /translation="LASTGSTGDSYDNAMAESINGLYKAEVIHRKSWKKRAEVELATL
                     TWVDWYNNRRLLGRLRHTPPAEAEKAYYASIGNDELAT"
     gene            272..505
                     /locus_tag="PN977_004897"
                     /pseudo
     CDS             272..505
                     /locus_tag="PN977_004897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013141603.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
     gene            582..890
                     /locus_tag="PN977_004898"
                     /pseudo
     CDS             582..890
                     /locus_tag="PN977_004898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000244358.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
     gene            complement(949..2259)
                     /locus_tag="PN977_004899"
     CDS             complement(949..2259)
                     /locus_tag="PN977_004899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDI6044822.1"
                     /translation="MFKPLTALTVGLSLALSGAALAKEKIDFMFPAPVDGKLTMEMTR
                     VIKQFNDSQQDVEVRGIFTGNYDTTKIKAESAQKAGQPPALVIMSANFTTDLALKDEI
                     LPMDELFKYGDQKAGNFLQTEFWPAMHKNAQVMGTTYAIPFHNSTPLLYYNKTMFDRA
                     GIQQPPQTWAELLADAKKLTDESTGQWGIMLPSTNDDFGGWIFSALVRANGGKYFNED
                     YPGEVYYNSPTTIGALRFWQDLIYKDKVMPSGVLNSKQISAAFFSGKLGMAMLSTGAL
                     GFMRENSKDFELGVAMLPAKEQRAVPIGGASLVSFKGISEAQKKAAYRFLTYLVSPEV
                     NGAWSRFTGYFSPRKASYDTPEMKAYLQQDPRAAIALEQLKYAHPWYSTWETVAVRKA
                     MENQLAAVVNDAKVRPEAAVQAAQKEADALMKPYVDKTALSEVK"
     gene            complement(2252..3325)
                     /locus_tag="PN977_004900"
     CDS             complement(2252..3325)
                     /locus_tag="PN977_004900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDI6044823.1"
                     /translation="MLSLQNISKRFDGKPALSALSLDIHEGEFVVLVGPSGCGKSTLL
                     RLLAGLEPVSEGQIWLHDENITATTPRDRNFAMIFQNYALFPHLSVRDNITFGMKVRK
                     EEKSSWQPRVDKVAQMLQLEALLDCKPAKLSGGQRQRVAMARAIVRNPRLFLMDEPLC
                     NLDARLRSEVRDSIMALHQQLKTSTIYVTHDQTEAMSMADRIVVMNGGHVQQVGHPEY
                     LYANPANLFVAGFIGSPAMNLLSLPCANGEVLLGEQRHPLPPRHRDQTRVWLGVRPEH
                     ITDHVEEDHLRLPATVLQRELMGADYLLHVSTPIGTLRFSRRNRGKVPEKDESLPIGF
                     SPADVHLFHAETQHNLQMETDHV"
     gene            complement(3331..4155)
                     /locus_tag="PN977_004901"
     CDS             complement(3331..4155)
                     /locus_tag="PN977_004901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase"
                     /protein_id="HDI6044824.1"
                     /translation="MLLAHISDTHFRSRGEKLYGFIDVNAANADVVSQLNALRERPDA
                     VVVSGDIVNCGRPEEYQVARQILGSLNHPLYLIPGNHDDKAHFLEHLHPLCPQLGNDP
                     QNMRYVVDDFATRLLFIDSSHAGTSKGWLTDETIGWLEAQLFEGGDKPATIFMHHPPL
                     PLGNAQMDPIACENGHRLLELVERFPSLTRIFCGHNHSLTITQYLQALISTIPGTVHQ
                     VPYCHEDTRSYYDLSPASCLMHRQVGEQWVSYQHSLAHHAGPWLYDENISCPTEER"
     gene            complement(4166..5053)
                     /locus_tag="PN977_004902"
     CDS             complement(4166..5053)
                     /locus_tag="PN977_004902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HDI6044825.1"
                     /translation="MSADISPLLVHTPAAARPLWLRLRRSSPFTLTVLMCCLALLWVS
                     PFIWMLTTSFSATTFGDDMASLLPRLPLTLDNFRDAWNSADWLSLYANTIIFTFGTFF
                     VQLLTITTAGYVFACHEFRGKQTLFLLFLVQLMIMPVVMMVPNMLTLKTFGLLNTLIG
                     VMMPYFTSAFGVFLMRKAFLAIPKELEEAALMEGCSWWQVLFRVLLPMSWPSVLAFAT
                     VSITYHWNEYLWPLMMLNDPDKQVLTVGLVSFAMGAESGGQWGTIGAGTLMVCLPLML
                     AFILFQKQFLRSFGFSGIK"
     gene            complement(5043..5921)
                     /locus_tag="PN977_004903"
     CDS             complement(5043..5921)
                     /locus_tag="PN977_004903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDI6044826.1"
                     /translation="MCVTMRKTWLPWLILSPSLLFLLLFTWFPLGRSVYDSLFDTRMA
                     NAQYVGLDNFARLFADNVFWQSLGNNLLYILLTVMPGVTLALLLAVALSENHRVNRWL
                     RTAFFFPMIIPMVSAAALWLFIFMPGLGLLDHYLAKLFGPMNNNWLGRSNSALLALAL
                     IGVWKFAGYYMLFFLAGLQSIPASTREAALMEGATRTQVFFKVTLPLLRPTLSFVITT
                     ALIYSITQIDHVAVMTRGGPDNATTVLLYYIQNLTWDTHDLGKASAATFLTLAGLFAF
                     SLINLKLLEKGAHYER"
BASE COUNT         1286 a         1652 c         1864 g         1210 t
ORIGIN      
        1 gctggcatca acagggagta caggcgactc gtatgacaac gcgatggcgg agagcattaa
       61 tggtctttac aaagcggagg taatacaccg taagagctgg aaaaaacgtg cagaagtgga
      121 actggccaca ctcacgtggg tggactggta taacaatcga cgattgctgg gaaggctgag
      181 gcatactcct ccggcagaag cagaaaaagc gtattatgct tccatcggaa atgatgagct
      241 ggcaacctga gttcacagat aaaacactct ccaggaaaac cggggcggtt cactggcgct
      301 caatattatt gttctgtgga acactattta catggatgca gcaattcagc atctcaggcg
      361 tgaggagtac ccggtcctgg attcagacgt tgagaaactg tcaccattac agtgcgggca
      421 tattaatatg caagggcgct attcatttat agtgccggaa tcagtcagta agggtgagct
      481 gagggcgttc aatgaagatg tatgatttat tgattaatag ataattggct attttaatta
      541 acctcttaac ggcgttactg ttccattgct ccccaaaccc ctttatagcg gtacgggtgc
      601 tgggcctgcg gcagggaggt atcagtgaag aaacacaaaa tgacagctgt gagaaaatac
      661 tggcgccgac agagtggaaa ttgctgtggg ttaagctgga aggaaaaccg cttccgtcac
      721 agacgccgac actgaaatgg gcctgtctga aactggcgaa actggggcga tggtatgaca
      781 gcaaacgcac aggccgcccc ggttgggtag ttatgtggga tggctggttc agacttcagg
      841 atatggttga gggttacctg gtgatgaagt ctcttgatca ggagatctga tcaagagaca
      901 gccctcaggg agagggccag gatgagggtg acgatgacgg gtaacgcgtc acttcacttc
      961 cgacaacgcg gtcttatcca catagggttt cattagcgcg tcagcttcct tctgcgccgc
     1021 ctgtaccgcg gcttccggcc ttactttggc atcgttgacc accgccgcca gctggttctc
     1081 catcgcctta cgcacggcga cggtctccca ggtggagtac catggatggg cgtacttcag
     1141 ttgctcaagc gcaatcgccg cccgcggatc ctgctgcaga taggccttca tctctggcgt
     1201 atcgtatgac gccttgcgcg gcgagaagta gccggtgaag cggctccatg cgccgttcac
     1261 ctcggggctc accagatagg tcaggaaccg atacgctgct ttcttctgcg cctcgctgat
     1321 gcctttaaaa cttacgaggc tggcgccgcc gatcggcact gcgcgctgct ctttggctgg
     1381 cagcatagct accccaagct caaagtcttt gctgttctca cgcataaagc ccagcgcgcc
     1441 ggtgctgagc atcgccatcc cgagcttgcc ggagaagaac gcggcgctga tctgtttcga
     1501 gttcagcacc ccggacggca tcaccttgtc tttgtagatc agatcctgcc agaagcgcag
     1561 ggcgccgatt gtggtcggcg agttgtaata gacctcgccc ggatagtctt cgttaaagta
     1621 ttttccgccg ttggcgcgca ccagcgccga gaagatccag ccgccgaagt cgtcgttggt
     1681 cgacggcagc atgatccccc actggcctgt gctctcgtcg gtcagctttt tggcatcggc
     1741 cagcaactcg gcccaggtct gcggcggctg ttggatcccg gcccgatcga acatcgtctt
     1801 gttgtagtag aggagcggcg tcgagttatg gaacgggatc gcataggtgg tgcccatcac
     1861 ctgggcgttc ttatgcatcg cgggccagaa ttccgtttgc agaaaattgc cggccttttg
     1921 atcgccatat ttaaacagct catccatcgg caggatctca tccttcagcg ccagatcggt
     1981 ggtgaagttg gcggacatga tcaccagcgc cggtggctgg ccggccttct gcgccgattc
     2041 ggctttgatc ttggtggtgt cgtagttgcc ggtgaagatt ccgcgcactt cgacatcctg
     2101 ctgcgagtcg ttgaattgct taatgacgcg ggtcatctcc atggtcagct tgccgtccac
     2161 cggcgccggg aacataaagt ctattttctc tttcgccagc gccgcgccgc tcagggcaag
     2221 gctgagccca accgtcaggg cggtgagggg tttaaacatg atctgtctcc atctgtaaat
     2281 tatgctgggt ctcagcatga aaaagatgca catcggcagg cgagaagccg atcggtagcg
     2341 actcgtcttt ctccggcacc ttgccgcggt tgcggcggct aaagcgcagg gtgccgatcg
     2401 gggtgctgac gtggagcaga taatcggctc ccatcagttc tcgttgcaga acggtggccg
     2461 gcaggcgcag atggtcctcc tccacgtggt cggtaatatg ttccgggcga acgcccagcc
     2521 agacgcgggt ctgatcccgg tggcgcggcg gcagcggatg gcgctgttct cccaacagta
     2581 cttcaccgtt ggcgcagggc agcgacagga ggttcatcgc cggcgaaccg ataaacccag
     2641 cgacgaacag gttggccggg ttggcgtaca gatactccgg gtgcccgacc tgctgcacgt
     2701 ggccgccgtt catcaccacg atgcggtcgg ccatcgacat cgcctcggtc tgatcgtggg
     2761 tcacatagat ggtgctggtt ttcagctgct gatggagggc cataatgctg tcgcgcactt
     2821 cgctgcgcag gcgggcgtcg aggttgcaga gcggctcgtc catcaggaac agccgcggat
     2881 tacgcacaat cgcccgggcc atcgccaccc gctggcgctg gccgccggag agcttcgccg
     2941 gtttgcagtc gagcagcgcc tccagctgca gcatctgcgc cactttatcc actcgcggct
     3001 gccagctgct tttctcttcc ttgcgcacct tcatgccgaa ggtgatgttg tcgcgcaccg
     3061 acagatgcgg aaacagggca tagttctgga agatcatcgc gaagttgcga tcgcgcggcg
     3121 tggtggcggt gatgttctca tcatgcagcc agatttggcc ttcgctgacc ggttccagcc
     3181 cggcgagcag gcgtagcagg gtgcttttcc cgcagcccga cgggccgacc agcaccacga
     3241 attcgccttc gtggatatcc agcgacaggg cgctgagcgc cggtttgccg tcgaaacgtt
     3301 tactgatgtt ttgcagactg agcatggcag ttagcgctct tccgttgggc aactgatgtt
     3361 ttcgtcgtac agccacggtc cggcgtggtg ggccagcgag tgctggtagc tcacccactg
     3421 ctcgccgacc tgacggtgca tcaggcacga ggccggggag agatcgtaat aggaccgggt
     3481 atcttcgtgg cagtacggca cctgatggac ggtgccagga atggtggaga tcagcgcctg
     3541 gagatactgg gttatggtca ggctgtggtt atggccgcag aagatgcgcg tcagcgacgg
     3601 aaaacgctcc accaactcca gcagacggtg gccattttcg caggcaatag gatccatctg
     3661 cgcattgccc agcggcagcg gcgggtggtg cataaagatc gttgccggtt tgtcgccgcc
     3721 ctcgaacagc tgggcttcca gccagccgat ggtctcgtcg gtcagccagc cttttgacgt
     3781 gccggcatga ctggagtcga taaacagcag gcgggtggcg aagtcatcca ccacatagcg
     3841 catattttgc ggatcgttgc ccagctgcgg gcacagcggg tgaagatgtt cgagaaaatg
     3901 ggctttgtcg tcgtggttgc cggggatcag atacagcgga tggttcaggc tgccgaggat
     3961 ctggcgggcc acctgatact cctccgggcg gccgcagttg acgatatccc cgctcaccac
     4021 caccgcgtcc gggcgctcgc gcagtgcgtt aagctgggag accacgtcgg cgttggcggc
     4081 attgacgtcg ataaagccat acagcttctc gccgcggctg cgaaaatggg tatcggaaat
     4141 gtgtgctaac agcatgaatc actccttatt tgatcccgga gaagccgaag ctccgcagga
     4201 actgtttctg aaaaaggata aacgccagca tcagcggcag gcagaccatc agcgtcccgg
     4261 cgccgatggt gccccactgg ccaccggatt cggcgcccat ggcgaaagag accagcccga
     4321 cggtcagcac ctgcttatcg ggatcgttga gcatcatcag cggccacagg tactcgttcc
     4381 agtggtaggt aatgctgacg gtagcgaagg ccagcaccga cggccaggac atcggcagca
     4441 gcacgcggaa cagcacctgc caccagctgc agccctccat cagcgccgcc tcctccagct
     4501 ctttcggaat ggcgaggaac gccttacgca ttaggaatac gccgaacgcc gaggtgaagt
     4561 agggcatcat caccccgatg agggtgttga gcagaccgaa ggttttcagg gtcagcatgt
     4621 tcggcaccat catcactacc ggcatgatca tcagctgcac caggaacagg agaaacagcg
     4681 tctgcttgcc gcgaaactcg tgacaggcaa agacgtagcc ggcggtggtg atggtcaaca
     4741 gctgcacgaa gaaggtgccg aaggtgaaga taattgtgtt ggcgtacagg ctcagccagt
     4801 cggcgctgtt ccaggcgtcg cggaagttat cgagagtcag cggcaggcgc ggcagcagcg
     4861 aggccatatc gtcgccgaag gtagttgcgc tgaatgaggt ggtcagcatc cagataaacg
     4921 ggctgaccca cagcagcgcc aggcagcaca ttaatactgt gagcgtaaag ggcgacgagc
     4981 gacgcaggcg cagccacagc ggacgcgcag cggcgggtgt gtggaccagc agcggcgaga
     5041 tgtcagcgct catagtgggc tcctttctcc agcagtttca ggttaatcag cgagaaggcg
     5101 aacagtccgg ccagggtcag gaaggtggct gcggaggctt taccaaggtc gtgggtatcc
     5161 caggtgagat tctggatgta atagagcagc acggtggtgg cgttatccgg cccgccgcga
     5221 gtcatcaccg ccacgtggtc aatctgggtg atggagtaga tcagcgcggt ggtgatcaca
     5281 aagctcagcg tcgggcgcag cagcggtagc gtgaccttaa aaaagacctg cgtgcgggta
     5341 gccccctcca tcagcgccgc ttcccgcgtc gaggccggaa tgctctgcaa cccggcgagg
     5401 aaaaacagca tgtagtagcc ggcgaacttc cacacgccga tcagcgccag ggccagcagc
     5461 gcgctgttgc tgcgccccag ccagttgttg ttcatcggcc cgaacagctt tgccagatag
     5521 tggtcgagca ggccgaggcc gggcataaag ataaacagcc acagtgcagc ggcgctgacc
     5581 atcgggataa tcatcgggaa gaagaaggcg gtgcgcagcc aacggttgac gcgatggttc
     5641 tcgctcaggg cgaccgccag cagcagggcg agcgtcaccc cgggcatcac cgtcagcagg
     5701 atatagagta ggttattgcc cagcgactgc cagaagacgt tatcggcaaa caggcgggca
     5761 aagttatcca gcccgacgta ttgcgcgttg gccatgcggg tatcaaacag gctgtcatac
     5821 accgaacgcc ccagcggaaa ccaggtaaac agcagtaaaa acagcaggga aggcgacagg
     5881 atcagccagg gaagccaggt ctttctcatg gttacgcaca tctcgttcag ccatttatcg
     5941 aagccctaat tgtgggaagc tatgattaca gaggtatgac ggggaaacga tggtttgatg
     6001 aagcaacggg ga
//
LOCUS       DAMZFT010000223         5983 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.223, whole genome shotgun sequence.
ACCESSION   DAMZFT010000223 DAMZFT010000000
VERSION     DAMZFT010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5983)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5983)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5983
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.223"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(<1..502)
                     /gene="entE"
                     /locus_tag="PN977_004904"
     CDS             complement(<1..502)
                     /gene="entE"
                     /locus_tag="PN977_004904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2,3-dihydroxybenzoyl)adenylate synthase EntE"
                     /protein_id="HDI6044827.1"
                     /translation="MSIPFTRWPEEFARRYREKGYWQDLPLTDILTRHAASDSIAVID
                     GERQLSYRELNQAADNLACSLRRQGIKPGETALVQLGNVAELYITFFALLKLGVAPVL
                     ALFSHQRSELNAYASQIEPALLIADRQHALFSGDDFLNTFVAEHSSIRVVQLLNDSGE
                     HNLQDAI"
     gene            complement(512..1687)
                     /gene="entC"
                     /locus_tag="PN977_004905"
     CDS             complement(512..1687)
                     /gene="entC"
                     /locus_tag="PN977_004905"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase EntC"
                     /protein_id="HDI6044828.1"
                     /translation="MDTSLAEEVQQTMATLAPNRFFFMSPYRSFTTSGCFARFDEPAV
                     NGDSPDSPFQQKLAALFADAKAQGIKNPVMVGAIPFDPRQPSSLYIPESWLSFSRQEK
                     QASARRFTRSQSLNVVERQAIPQQATFEQMVARAAALTATPQVDKVVLSRLIDITTDA
                     AIDSGVLLERLIAQNPVSYNFHVPLADGGVLLGASPELLLRKDGERFSSIPLAGSARR
                     QPDEVLDREAGNRLLASEKDRHEHELVTQAMKEVLRERSSELNVPSSPQLITTPTLWH
                     LATPFEGKANSQENALTLACLLHPTPALSGFPHQAATQVIAELEPFDRELFGGIVGWC
                     DSEGNGEWVVTIRCAKLRENQVRLFAGAGIVPASSPLGEWRETGVKLSTMLNVFGLH"
     gene            1876..2832
                     /gene="fepB"
                     /locus_tag="PN977_004906"
     CDS             1876..2832
                     /gene="fepB"
                     /locus_tag="PN977_004906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe2+-enterobactin ABC transporter
                     substrate-binding protein"
                     /protein_id="HDI6044829.1"
                     /translation="MRLAPLYRNALLLTGLLLSGIVAVQAADWPRQITDSRGTHTLES
                     QPQRIVSTSVTLTGSLLAIDAPVIASGATTPNNRVADDQGFLRQWSKVAKERKLQRLY
                     IGEPSAEAVAAQMPDLILISATGGDSALALYDQLSTIAPTLIINYDDKSWQSLLTQLG
                     EITGHEKQAAERIAQFDKQLAAAKEQIKLPPQPVTAIVYTAAAHSANLWTPESAQGQM
                     LEQLGFTLAKLPAGLNASQSQGKRHDIIQLGGENLAAGLNGESLFLFAGDQKDADAIY
                     ANPLLAHLPAVQNKQVYALGTETFRLDYYSAMQVLERLKALF"
     gene            complement(2836..4086)
                     /gene="entS"
                     /locus_tag="PN977_004907"
     CDS             complement(2836..4086)
                     /gene="entS"
                     /locus_tag="PN977_004907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin transporter EntS"
                     /protein_id="HDI6044830.1"
                     /translation="MNKQSWLLNLSLLKTHPAFRAVFLARFISIVSLGLLGVAVPVQI
                     QMMTHSTWQVGLSVTLTGGAMFVGLMVGGVLADRYERKKVILLARGTCGIGFIGLCLN
                     ALLPEPSLLAIYLLGLWDGFFASLGVTALLAATPALVGRENLMQAGAITMLTVRLGSV
                     ISPMIGGLLLATGGVAWNYGLAAAGTFITLLPLLSLPALPPPPQPREHPLKSLLAGFR
                     FLLASPLVGGIALLGGLLTMASAVRVLYPALADNWQMSAAQIGFLYAAIPLGAAIGAL
                     TSGKLAHSARPGLLMLLSTLGSFLAIGLFGLMPMWILGVVCLALFGWLSAVSSLLQYT
                     MLQTQTPEAMLGRINGLWTAQNVTGDAIGAALLGGLGAMMTPVASASASGFGLLIIGV
                     LLLLVLVELRRFRQTPPQMTASDS"
     gene            4197..5201
                     /gene="fepD"
                     /locus_tag="PN977_004908"
     CDS             4197..5201
                     /gene="fepD"
                     /locus_tag="PN977_004908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-siderophore ABC transporter permease"
                     /protein_id="HDI6044831.1"
                     /translation="MSGSVAVTRAIAVPGLLLLLIIATALSLLIGAKSLPASVVLEAL
                     SGTCQSADCTIVLDARLPRTLAGLLAGGALGLAGALMQTLTRNPLADPGLLGVNAGAS
                     FAIVLGAALFGYSSAQEQLAMAFAGALVASLIVAFTGSQGGGQLSPVRLTLAGVALAA
                     VLEGLTSGIALLNPDVYDQLRFWQAGSLDIRNLHTLKVVLIPVLIAGATALLLSRALN
                     SLSLGSDTATALGSRVARTQLIGLLAITVLCGSATAVVGPIAFIGLMMPHMARWLVGA
                     DHRWSLPVTLLATPALLLFADIIGRVIVPGELRVSVVSAFIGAPVLIFLVRRKTRGGA
                     "
     gene            5198..>5983
                     /gene="fepG"
                     /locus_tag="PN977_004909"
     CDS             5198..>5983
                     /gene="fepG"
                     /locus_tag="PN977_004909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter permease"
                     /protein_id="HDI6044832.1"
                     /translation="MIYVSRRLIITCLLLITACVVAGIWGLRSGTVTLETSQVFAALM
                     GDAPRSMTMVVTEWRLPRVLMALLIGAALGVSGAIFQSLMRNPLGSPDVMGFNTGAWS
                     GVLVAMVLFGQDLTAIALAAIAGGIVTSLLVWLLAWRNGIDTFRLIIIGIGVRAMLVA
                     FNTWLLLKASLETALTAGLWNAGSLNGLTWAKTSPSAPIIILMLIAAALLVRRMRLLE
                     MGDDTACALGVSVERSRLLMMLVAVVLTAAATALAGPISFIALV"
BASE COUNT         1339 a         1729 c         1628 g         1287 t
ORIGIN      
        1 taatcgcatc ctgcaagtta tgctcaccgc tgtcgttgag cagctgcacc acgcgaatgg
       61 aagaatgctc tgcaacaaat gtgttgagga aatcatctcc gctaaacagc gcatgctggc
      121 gatcggcaat cagtaacgcc ggttcaatct ggctggcgta ggcgttcagt tcactacgct
      181 gatggctgaa cagtgccagc accggcgcaa cgcccagttt cagcagcgcg aaaaaggtaa
      241 tgtaaagttc agcgacgtta cccagctgta ccagcgcggt ttcaccaggt ttaatgccct
      301 gacggcgcaa actacacgcg aggttatccg ccgcctgatt cagctcccga taactcaact
      361 gccgctcgcc gtcgataacc gcgatgctgt cgcttgcggc atgacgggtc agaatgtcgg
      421 tcagcggcaa atcctgccag tagccttttt cccgatagcg acgggcaaac tcttccggcc
      481 agcgggtgaa tggaatgctc atcctcactc cttaatgcaa tccaaaaacg ttcaacatgg
      541 tagaaagttt gacgcccgtt tcgcgccact cacccaacgg tgacgacgca ggcacaatcc
      601 ccgctccggc aaacagacgc acctgatttt cccgcagctt cgcgcagcgg atggtcacaa
      661 cccattcgcc gttaccttcg ctgtcacacc aacccacaat gccgccaaac agttcgcggt
      721 cgaacggctc cagttcagca ataacctggg tcgcggcctg atgcgggaaa ccgctcagcg
      781 cgggggtcgg atgcagcaga caggccagag tcagtgcgtt ttcttgcgaa ttcgctttac
      841 cttcaaaggg agttgcgaga tgccacagcg tcggcgtggt aatcaattgt ggagaggaag
      901 gaacgtttaa ctcactgctg cgttctcgca gtacctcttt catcgcctga gtcaccaatt
      961 catgttcatg acgatctttt tctgaggcca acagacgatt acccgcttcg cgatcgagca
     1021 cttcatccgg ctgacgacgc gcggaaccgg ctaacggaat ggagctaaaa cgctcgccgt
     1081 ctttacgtag cagcagctcc gggctggccc ccagcagaac gccaccatca gccagcggga
     1141 catggaagtt gtaactaacc gggttttgtg caatcaaccg ttccagcaat acgccactat
     1201 caatggcggc gtcagtggtg atatcaatca accgtgacaa caccactttg tcgacctgcg
     1261 gcgtggcggt aagtgcggcg gcgcgggcaa ccatctgttc aaacgtggct tgttgcggaa
     1321 ttgcctgacg ttccaccacg ttcagcgact ggctgcgggt gaaacggcgt gcggaagctt
     1381 gtttttcctg acgggagaac gacagccagg attcgggaat atacagcgac gaaggctgac
     1441 gtggatcgaa gggaatcgcc ccgaccatca ccggattttt gatgccctgc gctttggcat
     1501 cggcaaacaa cgcggcgagt ttttgctgga agggactgtc gggcgaatcc ccgttcacag
     1561 ccggttcatc gaagcgggcg aaacatcctg acgtcgtaaa actgcggtac ggcgacataa
     1621 agaaaaagcg attgggcgca agtgttgcca tggtctgctg tacttcctca gccagtgacg
     1681 tatccatatc atcctccaca aaatgataaa ggctttaata atgattatca tttatatttt
     1741 cggtcgctaa cctaaaagca aacgcgcact atgtcaactc ttgaggtaac gcaatctgca
     1801 actcggggtt gcatctgttt gtgattacaa tgaaaatgag aagcattaac tctattaata
     1861 acaggaagct aatttgtgag actcgccccg ctctaccgca acgcccttct attaacagga
     1921 cttttgcttt caggaatagt cgcagttcag gccgccgact ggccgcgtca gattactgac
     1981 agccggggca ctcataccct ggaaagccag ccgcagcgta ttgtttccac cagcgtcacc
     2041 ctgactggct cactgctggc gattgatgct ccggtgatcg ccagcggcgc gaccacgccg
     2101 aataaccgcg tcgcggatga ccagggcttt ttacgccagt ggagcaaggt ggcgaaagaa
     2161 cgcaaactgc aacggctcta tatcggcgaa ccgagtgcag aagccgttgc cgcgcaaatg
     2221 ccggatctga ttttaattag cgcaaccggc ggggattcgg cgctggcgct gtacgatcag
     2281 ctttctacca tcgccccaac attaatcatc aattacgacg acaaaagctg gcagtcgctg
     2341 ttaacgcagc ttggcgaaat taccgggcat gagaaacaag cggcagagcg gattgcacag
     2401 tttgataagc aactggcggc ggcgaaagag caaatcaaat taccgccgca gccggtcact
     2461 gccattgtct ataccgccgc tgcacacagt gccaatctct ggacgccgga atcagcacaa
     2521 gggcagatgc tggaacaact cggctttacg ttggcgaaat tgcccgcagg gttaaacgcc
     2581 agccaaagcc agggcaaacg ccatgacatc attcagcttg gtggagaaaa tctggctgca
     2641 gggttaaatg gcgagtcact attcctgttc gctggtgatc agaaagatgc cgatgcgatt
     2701 tatgccaatc cactgctcgc gcacctgccc gcagtacaaa acaagcaggt ttatgcgctg
     2761 ggaaccgaga cgttccgcct ggattactac agcgccatgc aagtgctgga acgacttaag
     2821 gcgctgtttt aagcattaac tgtcggacgc tgtcatctgc ggcggcgtct ggcgaaaacg
     2881 tcgcaactcc accagcacca gcaataacaa cacgccgata atcaacaaac caaaaccgct
     2941 cgcgcttgcg gaagcaaccg gtgtcatcat cgcgcccaaa ccgcccagca gcgctgcgcc
     3001 tatggcatcg cccgtcacgt tttgcgccgt ccacaaaccg ttaatccgcc ctaacatcgc
     3061 ttccggggtt tgcgtttgca gcattgtata ttgcagcaac gaactaaccg cgctcaacca
     3121 accgaacagc gccaggcaaa ccacgcctaa aatccacatc ggcatcaggc caaacagacc
     3181 aatggcaagg aacgatccca gcgtggagag cagcatcaat aaccctggtc gcgcactatg
     3241 tgccagcttc ccgctggtta acgcgccaat agccgcgccg agcggaatcg ccgcatagag
     3301 aaaaccaatc tgtgccgccg acatctgcca gttgtcagcc agcgccggat acaggacccg
     3361 caccgcgctc gccatcgtca ataaaccacc cagcaacgca atccctccca ccagcgggct
     3421 ggcgagtaga aaacgaaatc ctgccagtaa tgatttcaac ggatgctcac gcggctgcgg
     3481 cggtggtggc aacgccggaa ggcttaacaa cggtagcaag gtaataaacg tgcccgccgc
     3541 cgccagtccg tagttccagg ctacgcctcc ggtcgccagc aataaaccgc caatcatggg
     3601 cgaaatcacc gatcccagac gcacggtcaa catggtgatc gccccggcct gcattaagtt
     3661 ttcacgccct accagtgcag gtgtcgccgc cagtagcgcc gtaacgccaa gcgatgcgaa
     3721 aaaaccatcc cataaaccaa gtaaatatat tgccagcaat gacggctccg gcagcagcgc
     3781 gttcaggcac agtccaatga agccaatgcc acaggtgccg cgcgccagca aaatcacttt
     3841 tttgcgctca tagcgatccg ccagcacacc gccgaccatc aaaccgacaa acatcgcccc
     3901 gccggtcagc gtcaccgaaa gccccacctg ccaggtagaa tgcgtcatca tctggatctg
     3961 caccggcacc gcgacgccga gcaaacccag agaaacaata gagataaaac gagcgaggaa
     4021 tactgcgcga aacgccgggt gcgttttcaa caggctgagg ttaagcagcc aggattgttt
     4081 attcattaca atgccttgcc atcaaatatt ttaaatacct attcttaggc tgcacatgct
     4141 aacatatcca aataagatcg ataacgataa ttaatttcat tatcatggaa gttcgtatgt
     4201 ctggttctgt tgccgtgaca cgcgccattg ccgtgcccgg attgctgtta ttactgatta
     4261 tcgcgacggc attaagcctg ctcattggag caaaatcact ccccgcttcc gtagtgctgg
     4321 aggccctctc cggcacctgc cagagcgccg actgcaccat cgtgctcgac gcacgactgc
     4381 cgcgcaccct tgccggttta ctggcaggcg gtgcgcttgg ccttgccggg gcgttaatgc
     4441 aaaccctcac ccgaaaccca cttgccgatc ctggtttgct tggcgtgaac gccggtgcca
     4501 gttttgccat tgtgctgggt gcggcgctgt ttggttactc ttccgcacag gaacaactgg
     4561 cgatggcctt cgccggggcg ctggtggcct cgttgattgt tgcctttacc ggcagccagg
     4621 gcggcgggca gttaagtccg gtgcgtttaa ccctggcggg cgtggcgctg gcggcggtgc
     4681 tggaagggct gaccagcggc atcgccctgc ttaatcctga cgtctacgat caactgcgtt
     4741 tctggcaagc cggttcactg gatattcgta atttacacac cttaaaagtg gtgctgatcc
     4801 cggtgctgat cgccggagca actgcgttat tactgagccg tgcactgaac agtttgagcc
     4861 ttggcagtga caccgcgacg gcgctgggca gccgcgtggc gcgcacacag ttgattggtc
     4921 tgctggcgat taccgtgctt tgtggtagtg cgacggcggt agttggcccg attgccttta
     4981 ttggcctgat gatgccgcat atggcgcgct ggctggtagg tgccgatcat cgctggtcgc
     5041 tgcccgtcac gctacttgcc acccctgccc tgctgctgtt tgccgatatc atcgggcggg
     5101 tgattgtgcc cggcgagtta cgcgtttctg tggtcagtgc gtttattggc gcaccggtgc
     5161 tgatcttcct cgtgcgacgt aaaacgcgag gtggcgcatg atttacgtct ctcgccgatt
     5221 aatcatcacc tgtttgctgc taattaccgc ctgtgtggtt gcaggtatct ggggattacg
     5281 cagcggtacc gtcacgctgg aaacctcgca ggtattcgcc gcgctgatgg gcgatgcgcc
     5341 gcgcagtatg acgatggtgg tcactgaatg gcgtttacca cgcgtgctga tggcgctgtt
     5401 aattggcgcg gctctgggcg tcagcggcgc gatttttcag tcgctgatgc gtaacccgct
     5461 cggtagccct gacgtaatgg gctttaacac cggggcgtgg agcggcgtgc tggtggcgat
     5521 ggtgctgttt ggtcaggacc tgacggctat cgcgctggca gcaatagcgg gcggcattgt
     5581 cacctcgctg ctggtctggc tgcttgcctg gcgtaacggt atcgacacct ttcggttgat
     5641 tattattggt atcggcgttc gcgccatgct ggtggccttt aatacctggc tgttgctgaa
     5701 agcgtcttta gaaacggcgc taacagcggg tttatggaat gccggatcgc tcaacggcct
     5761 gacgtgggca aaaacctcgc cttccgcacc catcattata ttgatgctaa ttgccgccgc
     5821 cttactggta cgccggatgc gcttgctgga aatgggcgat gataccgcgt gtgcgctggg
     5881 cgtcagcgtc gaacgttcgc ggctgttaat gatgctggtc gccgtagtgc ttaccgcagc
     5941 tgccactgcc ctggcggggc cgatttcctt tattgcttta gtc
//
LOCUS       DAMZFT010000224         5911 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.224, whole genome shotgun sequence.
ACCESSION   DAMZFT010000224 DAMZFT010000000
VERSION     DAMZFT010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5911)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5911)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5911
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.224"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            1..213
                     /locus_tag="PN977_004910"
     CDS             1..213
                     /locus_tag="PN977_004910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF987 domain-containing protein"
                     /protein_id="HDI6044833.1"
                     /translation="MTRGEAMRIHRQHPASRLFSFCTGKYRWHGSAEVYTGREVQDIP
                     GVLAVFAERRKDSFGPYVRLMSVTLN"
     gene            287..655
                     /gene="cbtA"
                     /locus_tag="PN977_004911"
     CDS             287..655
                     /gene="cbtA"
                     /locus_tag="PN977_004911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system toxin CbtA"
                     /protein_id="HDI6044834.1"
                     /translation="MSDTLPGTTLPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYL
                     ADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSGELNPRHQHTVTLYAKGLTCKAD
                     TLSSCGYVYLAVYPTPEMKN"
     gene            744..1117
                     /gene="cbtA"
                     /locus_tag="PN977_004912"
                     /pseudo
     CDS             744..1117
                     /gene="cbtA"
                     /locus_tag="PN977_004912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416509.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system toxin CbtA"
     gene            1114..1308
                     /locus_tag="PN977_004913"
     CDS             1114..1308
                     /locus_tag="PN977_004913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310834.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6044835.1"
                     /translation="MMTLEADSVNVQALDMGHIVVDIDGVNITELINKAAENGYSLRV
                     VDDRDSTETPATYASPHQLL"
     gene            1321..1434
                     /locus_tag="PN977_004914"
     CDS             1321..1434
                     /locus_tag="PN977_004914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001161660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF957 domain-containing protein"
                     /protein_id="HDI6044836.1"
                     /translation="MQGIVPLTTETALLSCIKQAREDVLTLRHLQLLHQNR"
     gene            complement(1723..1869)
                     /locus_tag="PN977_004915"
                     /pseudo
     CDS             complement(1723..1869)
                     /locus_tag="PN977_004915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080164.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            1923..2105
                     /locus_tag="PN977_004916"
     CDS             1923..2105
                     /locus_tag="PN977_004916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001016346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein"
                     /protein_id="HDI6044837.1"
                     /translation="MNHPVIGVVTKADLASMEQISLVKCWLREAGAHNVLVTSAVNNN
                     GVTELFALLHTEEGCR"
     gene            complement(2206..2535)
                     /locus_tag="PN977_004917"
     CDS             complement(2206..2535)
                     /locus_tag="PN977_004917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF496 family protein"
                     /protein_id="HDI6044838.1"
                     /translation="METTKPSFQDVLEFVRLFRRKNKLQREIQDVEKKIRDNQKRVLL
                     LDNLSDYIKPGMSVEAIQGIIASMKGDYEDRVDDYIIKNAELSKERRDISKKLKAMGE
                     MKNGEAK"
     gene            complement(2707..3765)
                     /locus_tag="PN977_004918"
     CDS             complement(2707..3765)
                     /locus_tag="PN977_004918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HDI6044839.1"
                     /translation="MRADKSLSPFEIRVYRHYRIVHGTRVALAFLLTFLIIRLFTIPE
                     STWPLVTMVVIMGPISFWGNVVPRAFERIGGTVLGSILGLIALQLELISLPLMLVWCA
                     AAMFLCGWLALGKKPYQGLLIGVTLAIVVGSPTGEIDTALWRSGDVILGSLLAMLFTG
                     IWPQRAFIHWRIQLAKSLTEYNRVYQSAFSPNLLERPRLESHLQKLLTDAVKMRGLIA
                     PASKETRIPKSIYEGIQTINRNLVCMLELQINAYWATRPSHFVLLNAQKLRDTQHMMQ
                     QILLSLVHALYEGNPQPVFANTEKLNDAVEELRLLLNNHHDLKVVETPIYGYVWLNME
                     TAHQLELLSNLICRALRK"
     gene            complement(3963..4436)
                     /gene="sbmC"
                     /locus_tag="PN977_004919"
     CDS             complement(3963..4436)
                     /gene="sbmC"
                     /locus_tag="PN977_004919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor SbmC"
                     /protein_id="HDI6044840.1"
                     /translation="MNYEIKQEDKRTVAGFHLVGPWEQTVKKGFEQLMMWIDSKNIVP
                     KEWVAVYYDNPDETPAEKLRCDTVVTVPNNFTLPENSEGVILTEISGGQYAVAVARVV
                     GDDFAKPWYQFFNSLLQDSAYEMLPKPCFEVYLNNGAEDGYWDIEMYVAVQPKHH"
     gene            complement(4555..5721)
                     /gene="dacD"
                     /locus_tag="PN977_004920"
     CDS             complement(4555..5721)
                     /gene="dacD"
                     /locus_tag="PN977_004920"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase DacD"
                     /protein_id="HDI6044841.1"
                     /translation="MKRRLIIAASLFVFNLSSGFAAENIPFSPQPPEIHAGSWVLMDY
                     TTGQILTAGNEHQQRNPASLTKLMTGYVVDRAIDSHRITPDDIVTVGRDAWAKDNPVF
                     VGSSLMFLKEGDRVSVRDLSRGLIVDSGNDACVALADYIAGGQRQFVEMMNNYAEKLH
                     LKDTHFETVHGLDAPGQHSSAYDLAVLSRAIIHGEPEFYHMYSEKSLTWNGITQQNRN
                     GLLWDKTMNVDGLKTGHTSGAGFNLIASAVDGQRRLIAVVMGADSAKGREEEARKLLR
                     WGQQNFTTVQILHRGKKVGTERIWYGDKENIALGTEQEFWMVLPKAEIPHIKAKYTLD
                     GKELTAPISAHQRVGEIELYDRDKQVAHWPLVTLESVGEGSMFSRLSDYFHHKA"
BASE COUNT         1546 a         1522 c         1376 g         1467 t
ORIGIN      
        1 atcacccgtg gcgaagccat gcgtattcac cgtcagcatc cggcatcccg tcttttttcg
       61 ttctgtaccg gcaaataccg ctggcacggc agtgctgagg tttataccgg tcgtgaagtg
      121 caggatattc ccggtgtgct ggccgtgttt gccgaacgcc gtaaggacag ttttggtccg
      181 tatgtccggc tgatgagcgt caccctgaac tgagtgggaa ttctgatgag cagaattatc
      241 gccactaccg gaccattctt agccgatttt ctgtaaggat tttatcgtgt cagacacact
      301 ccccgggaca acacttcccg acgacaatca cgaccgcccc tggtgggggc tgccctgcac
      361 cgtgacgccc tgtttcgggg cacgtctggt gcaggagggt aaccgcctgc attaccttgc
      421 cgaccgcgcc ggtatcagag gcctgttcag cgatgcagat gcgtaccacc tggaccaggc
      481 ctttccgctg ctgatgaaac aactggaact catgctcacc agcggtgaac tgaatccccg
      541 ccatcagcat accgtcacgc tgtatgcaaa agggctgacc tgcaaagccg ataccctcag
      601 cagttgtggt tacgtttatc tggctgttta tccgacgccc gaaatgaaaa attaactctc
      661 cagaatagcc ttctgctacg gcctggtgtt ttcaccacgc cacttttcca tttttatatc
      721 tgcatatcag gaaaatcttc agtatgaaaa cattacctgt attacccggg caggcggcca
      781 gttctcgccc gtctcctgtt gaaatctggc agatactgct gtcccgactg ctggaccagc
      841 actatggcct cacacagaat gacacacctt ttgccgatga acgtgtgatt gagcagcata
      901 ttgaggcagg catttcactg tgtgatgcgg tgaactttct cgtggaaaaa tacgcgctgg
      961 tgctaccgac cagccgggat tcagcgcctg tacccgctct cagttaataa acagcatcga
     1021 tatcctccgg gctcgcaggg cgaccggcct gatgacccgc gacaattaca gaacggtaaa
     1081 taacattacc ctgggtaagt atccggaggc gaaatgatga cactggaagc cgacagcgtt
     1141 aacgtacagg cgctggatat ggggcacatt gtcgttgaca ttgatggtgt taatatcact
     1201 gaactgatta ataaggccgc tgaaaacggt tattcactcc gcgtggtgga tgaccgtgac
     1261 tctaccgaaa caccggcaac ttatgccagc cctcaccagt tgctgtaaga caatgcagtg
     1321 atgcagggta tagtgccatt aaccacagaa acagcactgt tgtcctgtat taagcaggca
     1381 agagaagatg tccttaccct gcgccatctg cagcttctgc accagaaccg gtgaatctca
     1441 ctcaacaggc ttcatttccc ctgacgccag cctgaataca gctggcgttt tcatttataa
     1501 acagaaagga aaaccgataa taatggaatg gacgccccga ccatgaagca acggtaggat
     1561 attactgata aaccttccag tggaggtgcg ttatggccgt tttaacgatt agcattgacc
     1621 tgacaaaaaa tgaattccaa atccacggtc tgggtaggaa cagaaaaatc taagctcaga
     1681 aaacggatta aagtaagcgt caacggagca ccatattgac gcttatttat tggtgagaac
     1741 tacgttccat ggcaggagtt cgtcaacacg gttggagggc cattccggca gtatgctcag
     1801 aatatggcgc agatacgctt ccggatcgat accgttcaga cggcaggtgc ctgcttttcc
     1861 ggcaacaagc aggaatctca gcgctgaagc aggaattaga ggtaaaaacg ccctaccggg
     1921 ccatgaatca cccggtcatt ggagtagtaa ccaaagcaga tttagccagc atggaacaaa
     1981 tatctttggt gaaatgttgg ttacgggaag ctggagcgca caacgtgtta gtaaccagtg
     2041 cggttaataa caatggagtt acagaacttt ttgccttgct gcatacagaa gaaggctgcc
     2101 gctaatttcg ataggttctc agatgagaat gatgacggtg atacacagat aagaatcaat
     2161 gagcaatgag ggttgccggg caaccctcat tgaataaaac aggaattact tcgcttcgcc
     2221 gtttttcatt tcgcccatag ctttcagctt tttggagata tcgcggcgtt ctttggagag
     2281 ctcggcattt ttgatgatgt aatcgtcaac gcgatcttca tagtcacctt tcatgctggc
     2341 gatgatgccc tggattgctt caacgctcat ccctggcttg atgtaatcgc tcaggttgtc
     2401 cagcaacagg acgcgcttct ggttgtcacg gatctttttc tcaacgtcct gaatttcacg
     2461 ttgcagtttg ttcttacgac ggaacagacg aacaaattcc agtacgtcct ggaatgaagg
     2521 cttggtagtt tccattttta tacccctgat aatgtgagag tcggattcgt ttaagcaacc
     2581 gctattcgat agggccaaca ttactcatag ctgccgcaga tgacaatgct tttatccctt
     2641 ttcactatca taccctttat ccttgctgaa tcgaagcagc agcaagatga ttctgaagtt
     2701 caggaattat ttgcgcaagg cccggcaaat cagattcgat agcaactcaa gctgatgcgc
     2761 cgtttccatg ttcagccaca cataaccata gattggtgtt tccacaacct tcaggtcatg
     2821 gtggttatta agcaacagac gcagctcttc cacagcatcg ttcaattttt ccgtattggc
     2881 aaaaaccggc tgcggattac cttcgtacag cgcatgaaca aggctcagca gtatttgctg
     2941 catcatgtgc tgggtatcac gaagtttttg cgcgttcaat aacacgaaat ggctggggcg
     3001 cgtggcccag tatgcattga tttgcaactc cagcatacaa accagattgc ggttaatggt
     3061 ctggatacct tcatatatcg attttggaat acgggtttct ttgctggcgg gcgcaatcag
     3121 cccacgcatt ttcacggcat cggtcagcag tttttgtaga tggctttcca gacgtgggcg
     3181 ttcgagtaag ttcggtgaga atgcagattg atagacccga ttatactcgg tcagactttt
     3241 cgccagttga atgcgccagt ggatgaacgc ccgttgtggc cagataccgg taaacaacat
     3301 tgccagtaaa gagccgagga tcacatcgcc gcttcgccat aacgccgtat caatttcacc
     3361 tgtcggggaa cccacaacaa ttgccagcgt caccccaatc aataatcctt gatacggttt
     3421 cttgcccagc gccagccaac cgcaaagaaa catcgccgcc gcgcaccaga ctaacatcag
     3481 cggtaacgaa attaactcca gttgcagagc gataagacct aaaatcgaac ccaacaccgt
     3541 accgccaata cgttcgaagg cacgagggac aacgttcccc cagaacgaga ttggccccat
     3601 aatcaccacc atggtgacca gcggccaggt gctttccggg atagtaaaca ggcggataat
     3661 gagaaaagtg agcaggaatg ccagcgcgac ccgagtacca tgcacaatgc ggtaatggcg
     3721 gtatacccgg atttcaaacg ggcttaatga cttatcggca cgcacgcaga aactccgtaa
     3781 tgaagattaa aagcgctcat aacacatctg tcggccagaa ggcgcgcctc tgagttatcc
     3841 gacggacata ccgggaacag tgtaatttcc cggcaggagc gaaaaatttg aaaggcgcat
     3901 cttactcttt tcgctttcaa aaaaagagtg gtcttcgcgt caacacaccg ccctgagatg
     3961 aattagtgat gttttggctg caccgcaaca tacatttcga tatcccagta cccatcttcc
     4021 gcgccattgt tcaagtaaac ctcgaagcag ggctttggta acatttcata ggcactgtcc
     4081 tgcaagaggc tattaaagaa ctgataccag ggtttagcaa aatcatcacc gactacacga
     4141 gccaccgcca ccgcatactg accacctgaa atttctgtca gaatgacgcc ctcactgttt
     4201 tcggggagcg taaagttatt cggcaccgtc acgacggtgt cgcagcgtaa tttttcggcg
     4261 ggtgtttcat ctggattgtc gtaatagaca gcaacccact ccttcggcac aatatttttg
     4321 ctatctatcc acatcatcaa ctgctcaaag cctttcttta ccgtctgttc ccacgggcca
     4381 acgagatgga aacctgcaac ggtacgttta tcttcctgct taatctcgta gttcatgacg
     4441 cccccattaa tactgttttt atatacagta tagttgcaat attaaaacca caaggaatga
     4501 gtgttgatta tgcgagcaga ctcgcactcc tgccagtctg ctgcaaaaga aaggtcaggc
     4561 cttatggtgg aaataatcac tcagccggga aaacatgctg ccttccccga cagattccag
     4621 ggtaaccagc ggccagtgcg ccacctgttt atcacggtcg taaagttcaa tttcccctac
     4681 ccgctgatgg gcgctaattg gcgcggtgag ttctttacca tcaagggtat atttggcttt
     4741 gatatgtgga atttcggctt tcggtagcac catccagaac tcttgttccg ttcccagggc
     4801 gatattttct ttatcgccat accagatgcg ttccgtacca acctttttcc cacggtgcaa
     4861 aatttgcacc gtagtaaagt tttgttgacc ccaacgcagt aattttcttg cctcttcctc
     4921 acgacctttt gcgctgtcag cccccattac cactgcaatg agacgacgct gcccatctac
     4981 agccgaagca atgagattaa acccggcacc agaagtatga cccgttttca ggccgtcaac
     5041 attcatggtt ttatcccaca ataacccgtt acggttttgc tgggtgatac cgttccaggt
     5101 gaggcttttc tcactgtaca tatgataaaa ctcgggctcg ccgtggatga tagcgcgaga
     5161 aagcacagct aaatcataag ccgagctatg ctggccaggt gcatccagac catgcactgt
     5221 ttcaaaatgc gtatccttga gatgcagctt ctcggcatag ttgttcatca tttcaacaaa
     5281 ctgccgttgc ccaccggcaa tatagtcagc cagtgcaaca caagcgtcat ttccggaatc
     5341 cacaattaaa ccacggctta aatcacgtac cgatacgcga tcgccctctt tcaaaaacat
     5401 cagtgaagaa ccgacaaaca ccggattatc tttcgcccac gcatcacgcc ccacggtgac
     5461 aatatcgtct ggcgtaatgc gatgactatc gatagcacga tccacgacat agcccgtcat
     5521 cagctttgtc aggctggcgg gattgcgctg ttgatgctca ttacccgcgg tgagaatctg
     5581 accggtggtg taatccatca atacccagga cccggcatga atctctggag gctgaggtga
     5641 aaaaggaatg ttttccgccg caaaaccaga cgataaatta aaaacgaaca aagaagcagc
     5701 aataataaga cggcgtttca acagcaaacc ctcaggtgtt tcaaatagct gttcttttta
     5761 cggaaatact tatgaactgg ctggaataaa gtgcaagaaa atgtgactac tatctcattt
     5821 ttatctgaca tgatctgttg ccactcgctg ccaaattgtc gcgctaaagc tgattagcac
     5881 ggtgatattt gatactctgg cagacagcag a
//
LOCUS       DAMZFT010000225         5869 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.225, whole genome shotgun sequence.
ACCESSION   DAMZFT010000225 DAMZFT010000000
VERSION     DAMZFT010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5869)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5869)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5869
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.225"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..510
                     /gene="traF"
                     /locus_tag="PN977_004921"
     CDS             <1..510
                     /gene="traF"
                     /locus_tag="PN977_004921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003557916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraF"
                     /protein_id="HDI6044842.1"
                     /translation="TLIKNYDSSAFKGDRYHNTQNGINADIGASMDLDDNWTLGLVAQ
                     NLIPRSIETKEVHGVTETFRIRPQVTTGVSWHNDMFTTALDVDLTPASSFTSDSKRQF
                     AAVGAEFNAWKWAQLRAGYRQNMASNDGSAFTAGIGLSPFDVVHLDIAGLAGTDNTYG
                     AVAQLSVTF"
     gene            948..1241
                     /locus_tag="PN977_004922"
     CDS             948..1241
                     /locus_tag="PN977_004922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001402879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6044843.1"
                     /translation="MKTNKKDTKWYIFYRENSGEEILLEMSSFKECLSASKELMTPSN
                     YMICIERNGERIKRWDREIIAGSDKWINCPPDNFEILGELITIKKITKIDPQM"
     gene            complement(1291..2253)
                     /locus_tag="PN977_004923"
     CDS             complement(1291..2253)
                     /locus_tag="PN977_004923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001696717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1281 domain-containing protein"
                     /protein_id="HDI6044844.1"
                     /translation="MAEWCRNRFEITGKSVCLDVLIQWIEGNDAPRYRHAIQQSILLF
                     LAGCAGILKPVRTVSYPPYPGLVSHGAGSTTVASQAFEQWLGLLARDAFLDHETVKTI
                     DRLYHQTGTGAIRWSNLPDTAKEIIGGVLAQQYADWFGVVDVYERPDNACLWESLLTF
                     AEYSQPCDMLQVMPTRLATELNGSGGLLKGTGTTRSFYCRQFGMEYPLGQNVRWKRTG
                     LSTLILEFDTIWCPPSGVLVGEISSVFDCEIRHWYSEPKNGINGYDCYDGGEHMDSKT
                     EAEWPQSDHAEEHEQTHLYLVRDSQPGERATASAESSGATAKIM"
     gene            complement(2361..2798)
                     /locus_tag="PN977_004924"
     CDS             complement(2361..2798)
                     /locus_tag="PN977_004924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006859754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6044845.1"
                     /translation="MMNVKSVTEVDDAVVARVSEVLEFAFPGQKFEVLKVCDSGVYNM
                     INVYWLDGPTQAEVRFITRAFEGKNGLRFVHESREFSNEFVQECIDRLRQKYGHRNVP
                     PEVTVERYWKNDLWKIKTDRFPGTIEVAINEMGMETSKYRKVV"
     gene            complement(2874..3212)
                     /locus_tag="PN977_004925"
     CDS             complement(2874..3212)
                     /locus_tag="PN977_004925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001247171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6044846.1"
                     /translation="MRPDLNTLPGDSGCSVWFYDGMSQPRLLAGSIAGLLTDVTITSN
                     YRGDVTSEIHDVVQEWLATGRGNLADLKEELWYYNLYINPSADELMNANRRYGLGHTT
                     RLKGFINNAA"
     gene            complement(3280..3639)
                     /locus_tag="PN977_004926"
     CDS             complement(3280..3639)
                     /locus_tag="PN977_004926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001681641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6044847.1"
                     /translation="MYSRFITGYWHLQTQSALLAHLCQLEGELTILRAWQRLGITPVP
                     EDEDEPSPLYSILWDVGEALGWLLYDLEMENVPAPGTIFHEVFDRVISLGYETEPGIW
                     AESISTGKRYAAMPDEF"
     gene            complement(3837..4346)
                     /locus_tag="PN977_004927"
     CDS             complement(3837..4346)
                     /locus_tag="PN977_004927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000853152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HDI6044848.1"
                     /translation="MKTKTSAPAVYVGTWAKYNNGSIEGCWFNDLTVFETESDFLAAA
                     RKFHKDEKDPEFMYQDYENFPSGMASEYGFNWAYVEGYRLAREEGCEDAWYAWVSNTG
                     DTCFEQFRDAWLGGPYDSELEFAAQYVDECGFLSEVPDTVARYFDYEAFARDLFMELD
                     FVDGHVFHR"
     gene            complement(4487..4678)
                     /locus_tag="PN977_004928"
     CDS             complement(4487..4678)
                     /locus_tag="PN977_004928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001167182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6044849.1"
                     /translation="MQISAETRAVLRHYRQIINARRREDGMKALSTPQIVDEICEFML
                     SQPAVYLGGQFIMQKAGTR"
     gene            complement(4765..5151)
                     /locus_tag="PN977_004929"
     CDS             complement(4765..5151)
                     /locus_tag="PN977_004929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001696709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6044850.1"
                     /translation="MGLDIFFLGRDRVCVTDAVVSVPETREVGYFRKVNPLIAWFEKH
                     CGPIENAVERPVSRTELEALLSDLECLTPENCREFFPTTEGFFFGSQEYDQYYWKDVE
                     DVKSWVRRTLDSFDFERKILLLWAWW"
     gene            complement(5304..5705)
                     /locus_tag="PN977_004930"
     CDS             complement(5304..5705)
                     /locus_tag="PN977_004930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000470503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6044851.1"
                     /translation="MFIPLLFRAEMRLTPFFQRLRQRLCYPVSRVHTQHPWSSPQLRH
                     LYCTPWFVNATGNLAVRACIVSIDGSLRHCFEFREGSHSREYRFWFEALPLSVLRFNA
                     FTGRISDRRTLSAASRMAAQLTSFRYRFGLR"
BASE COUNT         1651 a         1545 c         1341 g         1332 t
ORIGIN      
        1 acgctgatca aaaactatga cagcagcgca ttcaaaggtg accgttacca caacacccaa
       61 aacggtatta acgctgatat cggtgccagc atggacctgg atgacaactg gacgctgggt
      121 ctggtcgcac agaacctgat cccacgcagc attgagacca aagaggtaca tggtgtcact
      181 gaaaccttca ggatccgtcc gcaggtaacg accggcgtat catggcacaa cgacatgttc
      241 accacggcac tggatgtgga tctgaccccg gccagtagct tcacatcaga cagcaaacgt
      301 cagtttgccg ccgtgggtgc tgagtttaat gcctggaaat gggcacagtt acgtgccggt
      361 taccgtcaga atatggccag caacgacggc agtgcattca ctgccggtat tggtctctcg
      421 ccgttcgatg tggtgcacct ggatattgcc ggtctggccg gtacggacaa tacatacggg
      481 gcagtggcac aactgagcgt cacattctga acacccagac agcatgccgc ataccgtcag
      541 cggcatgctg gcaactacac tgaaacaggg acaatgactg atgagaaaca ggaagagcac
      601 accggcagga ttgcagctgg gtttttcacc gttatcaaaa accattatgc ttgccagaat
      661 gcgtgatatt gaaggcggac gtcttcgcgt gggtaatgac cgggggagag attttactaa
      721 tgaagccgcg cagcaacttt cacaatacag gagggtaagc accatcattt caagacaaat
      781 ctggcactga ataagacggg caatatagcc gataatgcgt tgaatcattg gctacatcgt
      841 atacgtaaaa cagacatcat tgttctgtct gttgttctta cagaataaat tcctttattt
      901 actggaaatt tataaggttt ttatagttat acgccgagaa tacccgtatg aaaactaata
      961 agaaagacac aaaatggtac attttttacc gtgaaaattc aggcgaggaa attttgcttg
     1021 aaatgtcatc atttaaggaa tgtctttcgg catccaaaga attaatgacc ccatcaaatt
     1081 acatgatatg catagaaaga aatggcgaaa gaataaaacg ttgggacagg gaaatcatag
     1141 caggctctga caaatggatt aactgcccac ctgataactt cgaaatatta ggtgagctaa
     1201 taaccataaa aaaaataaca aaaattgacc cacaaatgta ataacgccag tcctgaaaaa
     1261 aagcgccaca gaatgtggcg cattgtttta ttacattatt tttgctgtcg cccctgagga
     1321 ctcagctgat gctgtggcac gttcacctgg ctgcgaatcc ctgaccagat acagatgagt
     1381 ctgttcgtgc tcctctgcgt ggtcagactg cggccattca gcttccgttt tgctgtccat
     1441 atgttcacca ccatcataac agtcataacc attgatcccg ttctttggtt cgctgtacca
     1501 gtggcgtatc tcgcagtcaa acaccgatga aatttccccc accagtacac ctgaaggcgg
     1561 acaccagatg gtatcaaact ccagtatcaa cgtactcagc ccggttcgtt tccaccggac
     1621 attctgcccc agtgggtact ccataccaaa ctggcggcag taaaaactgc gggtcgttcc
     1681 ggtgcctttc agcagaccac cacttccgtt cagctccgtg gccagccgcg ttggcatcac
     1741 ctgcagcata tcgcagggtt gcgaatactc cgcaaatgtt aacaacgact cccagagaca
     1801 ggcgttatca gggcgttcat acacatccac cacgccaaac cagtcagcat attgttgtgc
     1861 aagcacacca ccgattatct ccttcgccgt atcaggcaga ttactccacc ggatcgcgcc
     1921 tgtccctgtc tgatgataaa gacgatctat tgtcttcacc gtctcgtgat caaggaaggc
     1981 atccctggcc agtaatccca gccattgttc aaacgcctga ctggctacag ttgttgagcc
     2041 ggcaccgtga ctgaccagcc cgggataagg cggatatgac accgttctta ccggtttcag
     2101 tatccccgca cagcctgcaa gaaacagcag tatgctctgc tggatcgcat gacggtaccg
     2161 gggggcatca ttcccttcaa tccactgaat cagtacgtca agacagactg acttaccggt
     2221 gatttcaaaa cgatttctgc accattcagc cataatcaat gctcctctgg taaatgaaga
     2281 gggcattgtc ccgtgaggaa caatgccctc acgggaaaag cggaattgtc acattccgcc
     2341 atcgtcaaaa gtccagccgg ttatacaacc ttccggtatt tactggtttc catccccatt
     2401 tcgttgatgg caacctcaat agtgcccggg aaacgatctg ttttaatctt ccacagatcg
     2461 tttttccagt aacgctccac ggtcacctct ggcggaacgt tgcgatgccc gtacttctgg
     2521 cgaagccggt caatgcactc ctgcacaaat tcattactga attcgcggga ttcgtgcaca
     2581 aacctcagcc cgtttttgcc ttcaaacgca cgggtgataa accgcacttc agcctgtgtc
     2641 gggccgtcca gccagtacac attaatcatg ttgtaaacgc cggaatcgca taccttcagc
     2701 acttcaaatt tctgccccgg aaatgcgaat tccagaactt cactgacacg ggcaaccaca
     2761 gcgtcgtcaa cctcagtcac agacttaacg ttcatcattc tgctcctctc tgtatgaaaa
     2821 tcaccgaggg aacagccccg tgaggagctg agccctcacg ggatggcggt gtgtcacgcc
     2881 gcgttattga taaagccttt cagcctggtg gtatgtccga ggccataccg cctgttggca
     2941 ttcataagtt cgtctgccga cggatttatg tagaggttgt agtaccagag ttcttccttt
     3001 aaatccgcca ggttacccct gccggtcgca agccactcct gcaccacgtc atgaatttca
     3061 gatgtgacgt caccacggta attcgatgtg atcgtaacat ccgtcagtaa cccggcaata
     3121 ctgcccgcca gcagacgcgg ttgagacatt ccgtcataga accagacgct gcagccggaa
     3181 tctccgggta aagtattcaa atcgggtctc atgaaatttc tcctgaaaaa gccgggaccc
     3241 gtcccacagg gatgagtccc tgtgggaatg tggaaaacat taaaattcgt caggcattgc
     3301 agcgtagcgc tttcccgtac tgatactttc agcccagata ccgggctcag tctcataccc
     3361 cagagatata accctgtcga atacctcgtg gaaaatcgtt cccggtgcag gaacattttc
     3421 catctccagg tcatacagca accagcccag agcctcacca acatcccaca aaattgagta
     3481 aagtggtgaa ggctcatctt catcctccgg caccggggta attccgagac gttgccaggc
     3541 gcgaagtatc gtcagttcac cttcaagctg acagagatgt gccagcaaag cggactgcgt
     3601 ctgaagatgc cagtaaccag taataaaacg cgaatacata aaaccccctg tctgattctt
     3661 tctgatgaaa gagcacaccg tccgaaggaa cgatgcctcc gggcggtgaa caacagagtt
     3721 caggttgttc atccgcgatg attaaaattg tcaacaacgg taaaaaaaac cagcagaatg
     3781 tatgcggaaa tcacatccgc cagtacacgg cttatatgcg ccctcatggc tgtgagttac
     3841 cgatggaaaa cgtgtccgtc gacaaaatcc agttccatga acaaatcgcg cgcaaaagcc
     3901 tcataatcaa aatagcgtgc aacagtatca ggtacctctg acagaaaacc gcattcatcc
     3961 acgtactgcg cggcgaactc gagttcactg tcataaggac caccaagcca cgcatcccgg
     4021 aactgttcga aacaggtatc accggtatta gaaacccatg cataccatgc atcttcacag
     4081 ccttcttcac gggcaagacg ataaccttca acataagccc agttgaaacc atattctgaa
     4141 gccattcccg aaggaaagtt ttcataatcc tggtacataa attcaggatc tttttcatcc
     4201 ttatgaaact tacgggccgc agctaaaaaa tcgctttcag tttcaaaaac ggttaaatca
     4261 ttaaaccagc atccttctat gctgccattg ttgtatttag cccaggtacc aacatataca
     4321 gcaggtgcag aagttttcgt tttcatgaga gtgtccttaa tgaaaagggt tcactcccac
     4381 cgtacggtat gagagtgaac cgtacggtta acagtaaaat cagacccgcc tcaccatgtg
     4441 gtggcgaaag cataacggtc tgttgaaaag ccatgaacaa agacagctat cttgttccgg
     4501 ccttctgcat gatgaactgc ccccccagat acactgctgg ctggctgagc ataaactcac
     4561 agatctcatc aacgatttgc ggcgtgctga gagctttcat gccatcttca cgccgtctgg
     4621 cgttaatgat ctgtctgtag tgacgtaaaa cagcgcgagt ttctgcactt atttgcataa
     4681 tctcattctc cggaataaaa gcaggccaga gctgtcgggc ctctgcgaat aatcacatgg
     4741 ttaaaaacaa ggactgagaa atagttacca ccatgcccag agcaacagta tttttcgttc
     4801 aaaatcaaac gaatcaagcg tgcgtctgac ccacgacttc acgtcctcaa catccttcca
     4861 gtaatactgg tcatactcct gagagccaaa aaagaatcct tctgttgtgg ggaagaattc
     4921 ccggcagttc tccggcgtaa ggcattcaag atcgctcagt agtgcctcca gttccgttct
     4981 ggaaaccgga cgctccacgg cattttcaat cggtccacaa tgcttctcaa accaggcaat
     5041 cagtgggtta accttgcgaa agtaccccac ttcccgcgtt tccggtaccg ataccactgc
     5101 gtcggtaaca caaacacgat cacgcccaag gaaaaaaata tcgagtccca taaacaatct
     5161 ccagaaacag agagaccgtt ccccgaacgg aagcggtccc ggacgggtta aggaaaaatc
     5221 agctttgtca tcgcgttctg aatggcgcga tcagacaacg ctgctcgtag agaaaatgac
     5281 ggtcataacg acctcaaaca gcttcagcgc aaaccgaagc gatagcgaaa ggaagtaagc
     5341 tgcgcggcca tacgggaggc agcagagagt gtacggcgat cggatattcg accggtaaaa
     5401 gcgttaaaac gcagtaccga caaaggaagc gcctcaaacc agaaacggta ttcacggctg
     5461 tgagagcctt cacggaactc aaagcagtgt cgcaaagaac cgtcaatgga aacaatgcag
     5521 gcacggactg ccagattgcc ggtggcgtta acaaaccacg gcgtgcagta aaggtgccgc
     5581 aactgtggag aagaccaggg atgttgtgta tgaacacgac tcaccggata acacagacgt
     5641 tgtctgagtc gctgaaagaa aggtgtcagg cgcatctctg cgcgaaacag aagtggaata
     5701 aacatcggaa cctccggata aaagacggac gttccgccct gacggactgg aaaatccgtc
     5761 aggtaatgct gactggtgac aatacagcca gcagagttaa ttaagaaatg taaccgataa
     5821 atccggtatt ggtagtaacg tacctcaatc accagtggtg atatccgtt
//
LOCUS       DAMZFT010000226         5662 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.226, whole genome shotgun sequence.
ACCESSION   DAMZFT010000226 DAMZFT010000000
VERSION     DAMZFT010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5662)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5662)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5662
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.226"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..165
                     /locus_tag="PN977_004931"
     CDS             <1..165
                     /locus_tag="PN977_004931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000190302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HDI6044852.1"
                     /translation="RMPNEEDVLVYCSDTKEQMVGFHKGKGLFQFFYMNGVEGVCEPS
                     HWMPLPEPPL"
     gene            262..441
                     /locus_tag="PN977_004932"
     CDS             262..441
                     /locus_tag="PN977_004932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001277760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Eag protein"
                     /protein_id="HDI6044853.1"
                     /translation="MSCPKCGSGNIAKEKTMRGWSGDYVCCDCGYNDSKDAFGERGKN
                     EFVKINKDREGNGKS"
     gene            555..773
                     /locus_tag="PN977_004933"
     CDS             555..773
                     /locus_tag="PN977_004933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001325331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HDI6044854.1"
                     /translation="MYLTLQEWNARQRRPRSLETVRRWVRECRIFPPPVKDGREYLFH
                     ESAVKVDLNRPVTGSLLKRIRNGKKAKS"
     gene            751..1821
                     /locus_tag="PN977_004934"
     CDS             751..1821
                     /locus_tag="PN977_004934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDI6044855.1"
                     /translation="MGRRRSHERRDLPPNLYIRNNGYYCYRDPRTGKEFGLGRDRRIA
                     ITEAIQANIELFSGHKHKPLTARINSDNSVTLHSWLDRYEKILASRGIKQKTLINYMS
                     KIKAIRRGLPDAPLEDITTKEIAAMLNGYIDEGKAASAKLIRSTLSDAFREAIAEGHI
                     TTNPVAATRAAKSEVRRSRLTADEYLKIYQAAESSPCWLRLAMELAVVTGQRVGDLCE
                     MKWSDIVDGYLYVEQSKTGVKIAIPTALHVDALGISMKETLDKCKEILGGETIIASTR
                     REPLSSGTVSRYFMRARKASGLSFEGDPPTFHELRSLSARLYEKQISDKFAQHLLGHK
                     SDTMASQYRDDRGREWDKIEIK"
     gene            1956..3239
                     /locus_tag="PN977_004935"
     CDS             1956..3239
                     /locus_tag="PN977_004935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative acyl-CoA thioester hydrolase"
                     /protein_id="HDI6044856.1"
                     /translation="MNTFSVSRLALALAFGVTLTACSSTPPDQRPSDQTAPGTSSRPI
                     LSAKEAQNFDAQHYFASLTPGAAAWNPSPITLPAQPDFVVGPAGTQGVTHTTIQAAVD
                     AAIIKRTNKRQYIAVMPGEYQGTVYVPAAPGGITLYGTGEKPIDVKIGLSLDGGMSPA
                     DWRHDVNPRGKYMPGKPAWYMYDSCQSKRSDSIGVLCSAVFWSQNNGLQLQNLTIENT
                     LGDSVDAGNHPAVALRTDGDKVQINNVNILGRQNTFFVTNSGVQNRLETNRQPRTLVT
                     NSYIEGDVDIVSGRGAVVFDNTEFRVVNSRTQQEAYVFAPATLSNIYYGFLAVNSRFN
                     ASGDGVAQLGRSLDVDANTNGQVVIRDSAINEGFNTAKPWADAVISNRPFAGNTGNVD
                     DNDEVQRNLNDTNYNRMWEYNNRGVGSKVVAEAKK"
     gene            complement(3310..5570)
                     /locus_tag="PN977_004936"
                     /pseudo
     CDS             complement(3310..5570)
                     /locus_tag="PN977_004936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706815.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydratase"
BASE COUNT         1524 a         1379 c         1379 g         1380 t
ORIGIN      
        1 cgaatgccaa acgaagaaga tgttttggtt tattgctcag acactaaaga gcagatggta
       61 gggtttcaca aaggtaaagg gttatttcaa ttcttttaca tgaatggtgt tgagggggta
      121 tgtgagccgt cacactggat gccgctacca gagcctccac tttgaaagcg aagcttatac
      181 atatctttta catcagcaat ctattgttaa tctccaatca atgttacgtt gtcatctcac
      241 tcatgctttg gaggtagtga tatgtcttgt ccaaaatgcg gttctggaaa tattgcaaaa
      301 gaaaaaacaa tgcgtggatg gtctggtgat tatgtgtgct gcgattgcgg atacaacgac
      361 tctaaagacg catttggaga gcgtggtaaa aacgagtttg tcaaaattaa taaagatcgc
      421 gaaggcaacg gaaaaagcta atttatttat tcatatatga aaacaatgta accaatattc
      481 gaattgaaga actgaaagaa caccaagccg cctgatggcg gttttttctt gcgtgtaatt
      541 gcggagactt tgcgatgtac ttgacacttc aggagtggaa cgcacgccag cgacgcccaa
      601 gaagccttga aacagttcgt cgatgggtac gcgagtgcag gatattccct cctccggtta
      661 aggatggaag agagtatctg ttccacgaat cagcggtaaa ggttgactta aatcgaccag
      721 taacaggtag ccttttgaag aggatcagaa atgggaagaa ggcgaagtca tgagcgccgg
      781 gatttacccc ctaaccttta tataagaaac aatggatatt actgctacag ggacccaagg
      841 acgggtaaag agtttggatt aggcagagac aggcgaatcg caatcactga agccatacag
      901 gccaacattg agttattttc aggacacaaa cacaagcctc tgacagcgag aatcaacagt
      961 gataattccg ttacgttaca ttcatggctt gatcgctacg aaaaaatcct ggccagcaga
     1021 ggaatcaagc agaagacact cataaattac atgagcaaaa ttaaagcaat aaggaggggt
     1081 ctgcctgatg ctccacttga agacatcacc acaaaagaaa ttgcggcaat gctcaatgga
     1141 tacatagacg agggcaaggc ggcgtcagcc aagttaatca gatcaacact gagcgatgca
     1201 ttccgagagg caatagctga aggccatata acaacaaacc ctgtcgctgc cactcgcgca
     1261 gcaaaatcag aggtaaggag atcaagactt acggctgacg aatacctgaa aatttatcaa
     1321 gcagcagaat catcaccatg ttggctcaga cttgcaatgg aactggctgt tgttaccggg
     1381 caacgagttg gtgatttatg cgaaatgaag tggtctgata tcgtagatgg atatctttat
     1441 gtcgagcaaa gcaaaacagg cgtaaaaatt gccatcccaa cagcattgca tgttgatgct
     1501 ctcggaatat caatgaagga aacacttgat aaatgcaaag agattcttgg cggagaaacc
     1561 ataattgcat ctactcgtcg cgaaccgctt tcatccggca cagtatcaag gtattttatg
     1621 cgcgcacgaa aagcatcagg tctttccttc gaaggggatc cgcctacctt tcacgagttg
     1681 cgcagtttgt ctgcaagact ctatgagaag cagataagcg ataagtttgc tcaacatctt
     1741 ctcgggcata agtcggacac catggcatca cagtatcgtg atgacagagg cagggagtgg
     1801 gacaaaattg aaatcaaata atgattttat tttgactgat agtgacctgt tcgttgcaac
     1861 aaattgataa gcaatgcttt tttataatgc caacttagta taaaaaagca ggcttcaacg
     1921 gattcatttt tctatttcat agcccggagc aacctgtgaa cacattttca gtttcccgtc
     1981 tggcgctggc attggctttt ggcgtgacgc tgaccgcctg tagctcaaca ccgcccgatc
     2041 aacgtccttc tgatcaaacc gcgccaggta cctcttctcg cccgattctg tcggcaaaag
     2101 aagcgcagaa tttcgatgct caacactatt ttgcatccct gacaccaggt gctgcagcgt
     2161 ggaatccttc cccgattacc ctgcctgcac aacctgactt tgttgtcggc ccggcgggta
     2221 ctcaaggtgt aacgcatacc acgattcagg cggcggtaga tgcggcaatt atcaagcgta
     2281 ccaacaagcg ccagtatatt gccgtgatgc ctggtgagta tcagggaacg gtgtatgtcc
     2341 ctgccgctcc gggtggaatt actctgtacg gtacaggtga aaaaccgatt gatgtgaaga
     2401 ttgggctttc ccttgatggt ggcatgagcc ctgccgactg gcgtcacgac gtcaacccgc
     2461 gcggcaaata tatgccaggt aaaccggcgt ggtatatgta cgatagctgc cagagtaaac
     2521 gcagcgacag tatcggtgtt ctctgctctg cggtcttctg gtcacaaaac aatggcctgc
     2581 aactgcaaaa cctgaccatc gaaaacacgc tgggcgatag cgtagatgcg ggtaaccatc
     2641 cggcggtggc actgcgtact gatggcgaca aagtgcagat caataacgtc aacattctcg
     2701 gtcgtcagaa caccttcttt gtcaccaaca gcggtgtgca gaaccgtctg gaaaccaacc
     2761 gtcagccgcg tacgctggtg accaacagct atattgaagg ggatgtggat atcgtttctg
     2821 gtcgcggcgc agtggtgttc gataacaccg aattccgcgt ggtgaactcc cgtacccagc
     2881 aagaagcgta tgtgtttgca ccggctacgc tgtccaacat ttactacggt ttcctcgccg
     2941 taaacagccg tttcaatgct tccggtgatg gcgtggcgca actgggccgc tcgctggatg
     3001 ttgatgccaa taccaacggt caggtagtga tccgtgatag cgccatcaac gaaggtttta
     3061 acacggcgaa accgtgggcc gatgcggtga tttccaatcg tccgtttgcg ggtaatactg
     3121 gcaacgttga tgataacgac gaagtacagc gcaatctgaa tgacactaac tacaaccgca
     3181 tgtgggaata caataaccgc ggcgtgggta gcaaagtggt tgcagaggcg aagaagtaaa
     3241 aaaagaaaac ccgatagtct gaaccatcgg gttaatgaaa ctgggcgcta tatttcatag
     3301 cgcctttttt tacatctgac ggtttttatt gaagttaatc aaactacccg ccttgataat
     3361 ctcgcgctct tcggccgtca ggctttccat gtaaagcgta atttccgtta ccggcgcatc
     3421 ttcatggatc acataacctt taaacgtcgt acccggatta tccagtgccg ctttaatgcc
     3481 agggatgtaa atgtaatccc ccacttcaaa ggttggcgct tccgccatct gcagcggtaa
     3541 catcccccag ttgatgacgt tagaacgata gcgtttagtc gcgtactcct cggcaatatt
     3601 cgccagaccg ccaatcacac gctgacaact cgccgcctgt tcacgcgcag aaccatcgcc
     3661 tggtttcacc gcatagacca tactgccaat ttcagtttgc agcggatcaa tatgctcctg
     3721 accagcaatc tgcttaatgc gcgcaaacac ctctgtcagc tcgctgacat ttcccgccag
     3781 acgctgattt tccagctcgg cagtcgcttt acttctgcca acataacctg gatcgcggcg
     3841 tgacagagta aactccgcca gaccaatcgg atttgaacga tatgaagagg tttcaccgga
     3901 aggaatcagt tcgtcggtgg tggtcacttc gtcgaggatc ttcgagcaca ctttgaggac
     3961 gatattgtca gtcagcgcac ccaattctgg ccagtcttta atgttcggtc cgtaaatcag
     4021 cggttgctgg gtcgcccctt tcacaaagcc ctgataaaca cggtttttat acggcgttac
     4081 atcgaaggcg tactccggca cgttgtccca gcaatccagc tcgctggcag aggttaaata
     4141 gccaccgttt gccgcagtcg cagcgataga acgagcgtcc atcaacgcca ccgctgacat
     4201 ctgcccatta gctggcttag agccttcgcg gttcgggaag ttacgcgtgg tgtggcgaat
     4261 actcaaaccg ttattgattg gtgtatcgcc cgcgccaaag catgggccgc agaacgcggt
     4321 tctgatgatt gcgcctgcgc caatcaaatc tgctaccaca ccttttttgg cgagatccat
     4381 aaacacgggc tgtgatgacg ggtaaaccgc taacgagaag gtgtcattgc cacaggattg
     4441 accgcgcagt gcattcgccg ccgcgatgac gttttcgtag ttaccgccag aacagcccgc
     4501 gataatcccc tgctgcactt tcaggcgacc gttttcgact ttatccagca gcgagagttt
     4561 ggctttaccg tgcgccacgc gctcggactc aatttcaatc tcacgcagaa tgtcggtcag
     4621 gttctggttc agcgtgtcga tttcatacac gttgctcggg tgaaacggca gcgcaatcat
     4681 tggtttgatg gcgcttaaat caacgctgat gcagccatcg tagtacgcca tcggttgagg
     4741 gttaagctgg cagtaatcct ggccgcgacc gtgcagcgcc agccagttat ggacttctta
     4801 tcggtttgcc agacagaact taaacaggtc gtttcagtgg tcatcacgtc aacgctgtta
     4861 cggaaatcgg tagagagcgc agcaacaccg ggacctacga attccatcac tttgtttttg
     4921 acgtagccgt ttttgaacac ggcaccgatg atagccagcg caacatcctg tggccccaca
     4981 tacggcgcag gttttccggt caaatgcacc gcaacaactc ccggatagtc gatatcccag
     5041 gtgtcattaa gcagctgttt taccaactca ccgccaccct caccgactgc cattgtccct
     5101 aatgcaccgt aacgggtatg gctgtctgac ccgagaatca ttttgccgcc gcccgccatc
     5161 atttcacgca tatattgatg gatgaccgca atatgcggtg gcacaaaaat accgccataa
     5221 cgttgcgctg ccgataagcc aaaaatatgg tcatcgccat taatagtacc gccgacggcg
     5281 cagagtgagt tatggcagtt ggtcagcaca tacggcagcg ggaaacgttc cataccggac
     5341 gctttagcgg tctgtacaat accaacaaag gtaatatcgt gagaggctaa tgaatcaaac
     5401 ttaattttaa gtttatccat attaccagac gtattatgag aagagagaat agaccaggca
     5461 atagtgcctt ttttggcttc ttctttttta atttcaccgg tgaaatgttc ttcggcaatt
     5521 atttcgttat tactggcgag aaacacgcct ttttcagata acttgatcat caaatgctcc
     5581 agatatctgc gcaccgtgaa gtgcgcagaa agataataaa tggatatact ctaaataatt
     5641 cgagttgcag gaaggcgaca ag
//
LOCUS       DAMZFT010000227         5650 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.227, whole genome shotgun sequence.
ACCESSION   DAMZFT010000227 DAMZFT010000000
VERSION     DAMZFT010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5650)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5650)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5650
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.227"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..930
                     /locus_tag="PN977_004937"
     CDS             <1..930
                     /locus_tag="PN977_004937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraF"
                     /protein_id="HDI6044857.1"
                     /translation="DDWKAFDRAVDSHSGVPQTAARLKERLQDFRNTHASAQAGVSAV
                     AALPGDTLAAALMLKTFGTVSVDGKVSDADLNYLESIADSGSQDVDKNRLTSQAFARA
                     ALITDVGVALATELETAGQKWSLGFTPKFQRVDLFNYNVLVKNYDSSAFKGDRYHNTR
                     NGINADIGASMDLDDNWTLGLVAQNLLPRSIETKEVNGVTETFRIRPQVTAGVSWHND
                     MFTTALDVDLTPASGFTSDSKRQFAAIGAEFNAWKWAQLRAGYRQNLASNDGSAFTAG
                     LGISPFDVVHIDVAGIAGTDNNYGAVAQLQFTF"
     gene            1070..1240
                     /locus_tag="PN977_004938"
     CDS             1070..1240
                     /locus_tag="PN977_004938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001597011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6044858.1"
                     /translation="MFNKNKTHIENKAFMVEPFRDPTANECNIVIANDICIRVALSPS
                     EVFQPFSKNTNQ"
     gene            1289..1741
                     /locus_tag="PN977_004939"
     CDS             1289..1741
                     /locus_tag="PN977_004939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079808.1"
                     /note="Members of this family include DotD of type IVB
                     secretion systems and TraH of plasmid conjugative plasmid
                     systems, both lipoproteins; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DotD/TraH family lipoprotein"
                     /protein_id="HDI6044859.1"
                     /translation="MNRMIPAGCLAVLCLNGCHLPPDNTAPTRKYGAASDVAVARHAQ
                     YQLWGQGIYSAALPERQSGGSLKAHSDRVSFDWEGDALELLNELARLRGQQFSYSGVR
                     LPLPVSLHVRDMTFADVLRLTEAQTAWRATLSQSAGRLHLSFMTPEKK"
     gene            1738..2574
                     /locus_tag="PN977_004940"
     CDS             1738..2574
                     /locus_tag="PN977_004940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000848138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HDI6044860.1"
                     /translation="MKTHLCLLLSALLLTGCAGQHHRSSAVMTENAPPPDIRAWLTPS
                     GNQDDGISDTRRQMLTDAGRTLGFRGGKAQRSWELIQALNARESTLNALYDFRPLISK
                     EGWLPPVIDEAQDVAHIRPDQIRTASRVWTILRPERFVSNPPGWRDWLLKGLSTTATP
                     GTEGRVVPEDRAQRRLWENALRQGWQEGRLNADLTLEANQKRLTRDYRGMMLYALLWR
                     QGMITRPDVTEQMQTVTGNGQKLITGDRVRRLKTHAEFTLQKSRWRPLVSTEGEKNDI
                     TR"
     gene            2558..3706
                     /gene="traJ"
                     /locus_tag="PN977_004941"
     CDS             2558..3706
                     /gene="traJ"
                     /locus_tag="PN977_004941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid transfer ATPase TraJ"
                     /protein_id="HDI6044861.1"
                     /translation="MISPDDFGTFPFSRFTAEEFRHFFAWCARHRVSDIDLTGGSPVS
                     VSRFGRRVRSSVNPLPSCIMMALSDELFGREVLPRVLSGHPVDRTIQVSGDASGRYGL
                     QRGQRVRLRCHLIQGTSATEEKAISVTMRVIPSDIPDILTMNIEPDLLDAMLRKTGLG
                     FICGETGSGKSTLAAALYRHILKNFPDRKIVTYEDPVEYILGGENDLLPPHQAEIGRD
                     VESFAAGLRSAVRRNPDIIGIGEIRDYETADAAVQAGNTGHFCIGTMHTKSPGETFAR
                     LLGLFPPEIRDSMAAATLSLVQFILVQVLVRTNDGGRQAVREYIVMTDELRNTLSGQS
                     HATWGHYIDEIIRQEKRRIRDQVLTMYQNDRIEKSEAALFIPAGEFPR"
     gene            3703..3993
                     /locus_tag="PN977_004942"
     CDS             3703..3993
                     /locus_tag="PN977_004942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001542523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HDI6044862.1"
                     /translation="MKREGFWRDVTPPLNIYGVPLPFTMIYLLLFPYPSKTTFWVCSG
                     ILLFFMLLGYSGWTVRTLCIRLYSLLQGKQASGRPWWYRHRTELPREWHGPE"
     gene            4057..>5650
                     /locus_tag="PN977_004943"
     CDS             4057..>5650
                     /locus_tag="PN977_004943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000842452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HDI6044863.1"
                     /translation="MKSRYRTWLAVPPEETEAVKNAVPPLNGRKAVAWDPEKKLWYAR
                     AGTELSLLERWLPRPQELSMDAGDPVTEFAQVLENAGLVIQGLPQMDGAIHRVATRDD
                     KKGAKSGAYKAYLDGRPAGWYRDYRSADDSPTNWVFSGGEQHDPLARLHLRAFAQQQR
                     DDNARKLQQQYNKQARYARSYINGLPQATAHEYLTRKGIRAAPGVRLNNKNELVIPFS
                     NGRGEIRSYQRIPVTGGKDARILKDSEKTGNWFTFGTPENGRPLLFAEGYATAASLHE
                     ATGLPVLMTVDAGNMIAVAENARQIWTDSPFVFCADNDHQREINKGVFSATKAAEVTN
                     GEVIIPAFTEEEKAQGLTDFNDLDASRGRDDFQHVMNAQLKHIGIPTPNSDTADHREA
                     VVIGNLIFTPVKDEKPQMSPENKQSTAPETVLATPDTPYDIHTEETENQAYEDDYAAY
                     ASLQSVTTETLSPDKPLENIASAADTGTENTSAATAGDQEDLNTDTTGTDTAHNTNTA
                     ETGTLPEPAEIQPVQKSSPEPTA"
BASE COUNT         1388 a         1630 c         1465 g         1167 t
ORIGIN      
        1 gatgactgga aagccttcga ccgtgccgtc gacagccact ccggggtacc gcagacagcg
       61 gcccgcctga aagagagact gcaggacttc cggaacaccc atgccagcgc acaggccggg
      121 gtatctgctg tggcggccct tcccggtgat accctcgctg ctgcactgat gctgaaaacg
      181 ttcgggactg tcagcgtcga cggtaaagtc agcgatgccg acctgaacta tctggaaagc
      241 attgcagaca gtggcagcca ggatgtggat aaaaacagac tgacctcaca ggcttttgcc
      301 cgtgccgcac tgatcaccga tgtgggcgtt gcgctggcca ctgaactgga aacggccgga
      361 caaaaatggt cactgggctt cacaccaaaa ttccagcgtg ttgacctgtt taactacaac
      421 gtgctggtta aaaattacga cagcagcgct ttcaaaggcg accgttacca caacaccaga
      481 aacggcatta acgctgatat cggggccagc atggacctgg atgacaactg gacactgggt
      541 ctggtcgcac agaacctgct cccccgcagt attgagacaa aagaagtgaa cggtgtcact
      601 gaaaccttca ggatccgtcc gcaggtgact gccggtgtct cctggcacaa cgacatgttc
      661 actacagcac tggatgttga cctgaccccg gccagtggct tcacatcaga cagtaaacgt
      721 cagtttgctg ccattggtgc tgaatttaat gcctggaaat gggcacagtt acgtgcaggc
      781 taccgccaga atctggccag caatgatggc agtgcgttca ccgccgggct gggtatctca
      841 ccatttgatg tcgtccatat tgatgtggcg ggtatagcag gcacagacaa caactacggt
      901 gccgttgcac aactgcagtt cacattctga tcatctctgc cccggtactg accggggtat
      961 ccagacctgc atcctcgcgt tttccgacca tcaaacgaca cagccagacc tgtagtgctg
     1021 cctctgatct ggctgttatc tgacatatca ttttcgcatg aggaaatgca tgtttaacaa
     1081 aaataaaacg catatcgaaa acaaagcctt tatggtggaa ccgttccggg atccgacagc
     1141 taacgaatgt aatatcgtga tagcaaacga tatttgtatc cgggtagcac tgtcaccctc
     1201 tgaggtgttc cagccattca gcaaaaacac gaaccagtaa atcctcccca caacgctttc
     1261 ttctttcact gctcaggaaa aatacaccat gaaccgtatg atacctgcgg gatgtctcgc
     1321 cgtactctgc ctgaacggct gccatctccc cccggataat acagctccga ccagaaagta
     1381 tggtgctgcc tctgatgtcg ccgtcgcccg gcatgcgcag tatcagctat gggggcaggg
     1441 aatttactct gcagcccttc cggagcgtca gtccggcggc agtctgaagg ctcacagcga
     1501 ccgtgtttcc tttgactggg aaggcgatgc cctggaattg ctcaacgaac tggcccgtct
     1561 gcgcggccag cagttcagct acagcggcgt ccgtctcccc ttacccgtct cactgcacgt
     1621 ccgcgacatg acctttgctg atgtccttcg tctcactgaa gcccagactg cctggcgggc
     1681 aacactctcg cagtcagccg ggcgtttaca tctgtctttt atgacgccgg agaaaaaatg
     1741 aaaacacacc tgtgtctcct gctgtctgct ctcctgctta caggctgtgc cggacagcat
     1801 caccgttctt ctgccgtcat gacagaaaac gcaccgcccc cggatatccg tgcatggctg
     1861 acgccctccg gcaaccagga tgatggtatt tcagataccc gtcgtcagat gctcacggat
     1921 gccggcagaa cgctgggatt ccgtggtgga aaagcacagc gctcatggga actgattcag
     1981 gcactgaatg cccgggaaag taccctgaat gccctgtatg atttccggcc tctcatcagc
     2041 aaggaaggct ggctgcctcc tgttatcgat gaagcgcagg atgttgcaca catcagaccg
     2101 gaccagataa gaacagcctc ccgtgtctgg accatcctcc gcccggaacg ctttgtcagt
     2161 aatccacctg gatggcgtga ctggctgctg aaaggactct caaccaccgc cacaccaggc
     2221 actgaaggcc gggttgtgcc ggaagaccgg gcacaacgca gactatggga aaacgcctta
     2281 cgtcagggat ggcaggaagg gcgcctcaac gcagatctca cactggaagc taatcagaag
     2341 cgcctgaccc gtgattaccg gggcatgatg ctttacgccc ttctgtggcg tcagggcatg
     2401 atcacacgtc cagatgtcac agagcagatg cagacggtta ccggcaacgg gcaaaaactc
     2461 atcactggcg atcgcgtccg ccgcctgaaa acacatgctg aatttacgct gcagaaatcg
     2521 cgctggcgcc ctcttgtcag caccgaagga gagaaaaatg atatcaccag atgacttcgg
     2581 taccttcccc ttctcccgtt ttacggcaga agagtttcgc catttttttg cctggtgtgc
     2641 ccgccaccgc gtcagtgata ttgatctgac cggcggttca ccggtatccg tcagtcgctt
     2701 tggacggcgg gttcgctcat ccgtaaatcc ccttccgtcc tgcatcatga tggcactgag
     2761 tgatgagctg tttgggcggg aggttcttcc ccgggttctg tcaggtcatc ccgtggatcg
     2821 taccattcag gtcagcggag atgcctccgg tcgctatggc ctgcaacgag gtcagcgtgt
     2881 tcgcctgcgc tgccacctga tacagggaac atcggcaaca gaagaaaaag ccatttccgt
     2941 caccatgcgg gtgatcccgt cagatattcc ggacatcctc actatgaata ttgaaccgga
     3001 tctgctggat gccatgctca gaaaaaccgg cctgggcttc atctgcggag aaaccggctc
     3061 cgggaaatcc acactggcgg cggcactgta tcgccacatt ctgaaaaatt tccccgatcg
     3121 caaaatagtg acctatgaag atccggtgga atacattctg ggcggagaga atgaccttct
     3181 gcctccccat caggccgaaa tcgggcgcga tgtggaaagt tttgctgcag gacttcgctc
     3241 tgccgttcgc cgtaatccgg acatcattgg tatcggtgaa atcagggatt atgaaacagc
     3301 agatgccgca gtccaggcgg gtaataccgg acacttttgt atcggcacca tgcacacaaa
     3361 atcacccgga gaaacctttg cccgcctgct gggattgttt cctcctgaaa tcagggattc
     3421 gatggctgca gccacgcttt ccctcgtaca gttcattctg gtgcaggtac tggtcagaac
     3481 aaatgacggc ggcaggcagg ctgtccggga atacatcgtc atgactgacg aactgcgcaa
     3541 tacgctctcc ggacaatccc atgcaacatg gggacattac attgacgaaa tcattcgtca
     3601 ggaaaaacgg cggataaggg atcaggttct gaccatgtat cagaacgacc gcattgagaa
     3661 atcagaagca gcgctcttca tcccggccgg agagttcccc cgatgaaacg cgaaggcttc
     3721 tggcgggatg taactccacc gctcaacatt tatggtgtcc ccctgccgtt taccatgatt
     3781 tacctgctgt tgtttccgta tccgtcaaaa acgacattct gggtgtgcag cggcattctg
     3841 ctgtttttta tgctcctggg atattccggc tggacagtcc gcaccctctg tatacgtctg
     3901 tacagcctgc ttcagggtaa gcaggcatca ggacgtccct ggtggtaccg acaccgcaca
     3961 gaattacccc gtgaatggca cggaccagaa tagccctctt ttgtccatca tcccggcgta
     4021 tcacaacgcc ctcatcttta atccccggag aacgacatga agtccagata tcgaacctgg
     4081 cttgctgtcc cgcctgagga gacagaagcc gtaaaaaatg cagtaccccc gttaaatggc
     4141 cgcaaagctg tcgcatggga tccagaaaaa aaactctggt atgcccgggc aggtaccgaa
     4201 ctttctctgc tggagcgctg gttaccccgg ccgcaggaac tgtcgatgga tgccggcgat
     4261 ccggtcaccg aatttgctca ggtcctggaa aacgccgggc tggttattca gggattgcca
     4321 cagatggatg gcgccataca cagagtcgcc acccgggatg acaaaaaagg ggccaaaagt
     4381 ggcgcataca aagcctatct tgatgggcgc cccgctggct ggtaccgcga ctaccggagc
     4441 gccgatgaca gtcccacaaa ctgggtattt tccggcggag aacaacacga tcctctggcc
     4501 agactgcatc tgcgggcatt cgctcagcaa cagcgcgatg ataacgcacg taaactgcag
     4561 caacagtaca acaaacaggc ccggtatgcc cgctcctata ttaacgggtt gccacaggca
     4621 acagcacatg aatacctgac gcgcaagggc atccgggccg cgcctggcgt gcggctgaac
     4681 aataaaaatg agctggttat tcccttcagc aacggccggg gggaaatacg ttcgtaccag
     4741 cgtattccgg tcaccggcgg gaaggatgcc cgcattctga aagactccga gaaaaccggc
     4801 aactggttca ccttcggaac cccggaaaat ggccgtcccc tgttatttgc ggaaggttac
     4861 gctactgccg cctccctgca cgaagccacc ggccttccgg ttctgatgac agttgatgcc
     4921 ggcaatatga ttgccgtggc agaaaatgcc cgccaaatct ggactgacag cccctttgtc
     4981 ttctgtgccg acaacgatca tcaacgggaa attaacaagg gggtttttag tgcaacaaaa
     5041 gccgcagaag tcacgaatgg cgaagtcatt attccggcct ttacggagga ggaaaaagca
     5101 cagggactga ccgatttcaa tgacctggac gccagccggg gacgggacga ctttcagcat
     5161 gtcatgaacg cacagttaaa acatatcggt atccccacac ccaacagtga cactgccgac
     5221 caccgtgaag ctgtcgtgat cggcaacctg atattcacac cggtaaaaga tgagaagcca
     5281 caaatgtccc ctgaaaacaa acagagcaca gccccggaaa cggtgctggc gacaccggat
     5341 acgccttatg acatccacac tgaagagaca gaaaaccagg catatgaaga tgactatgcc
     5401 gcctatgcat ccctgcagtc agtaactacg gagacgttat ccccggataa accactggag
     5461 aatattgcgt ctgccgctga taccggcaca gagaatacct ctgcggctac cgctggcgac
     5521 caggaagacc tcaatacaga caccacaggt acagacaccg ctcacaacac caacacagca
     5581 gaaacaggaa cgcttcctga acctgcagag attcagcctg ttcagaagtc ctcccctgaa
     5641 ccaacagcag
//
LOCUS       DAMZFT010000228         5603 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.228, whole genome shotgun sequence.
ACCESSION   DAMZFT010000228 DAMZFT010000000
VERSION     DAMZFT010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5603)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5603)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5603
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.228"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            1..900
                     /locus_tag="PN977_004944"
                     /pseudo
     CDS             1..900
                     /locus_tag="PN977_004944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001368704.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            980..1272
                     /locus_tag="PN977_004945"
                     /pseudo
     CDS             980..1272
                     /locus_tag="PN977_004945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312320.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
     gene            complement(1312..2346)
                     /locus_tag="PN977_004946"
     CDS             complement(1312..2346)
                     /locus_tag="PN977_004946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotriesterase"
                     /protein_id="HDI6044864.1"
                     /translation="MKDYLQTVTGPVAREDMGLTLPHEHLFNDLSSVVDAPCYPFSQR
                     LVDKKVTAEIQWALKHDPYCCADNMDRKPIEDVIFEINNFISLGGRTIVDATGSESIG
                     RDAQALREVALKTGLNIVASSGPYLEKFESQRIHKTVDELATTIDKELNQGIGDTDIR
                     AGMIGEIGVSPTFTEAEHNSLRAASLAQINNPHVAMNIHMPGWLRRGDEVLDIVLGEM
                     GVSPNKVSLAHSDPSGKDVAYQRKMLDKGVWLEFDMIGLDITFPKEGIAPGVQETADA
                     VAHLIELGYADQLVLSHDVFLKQMWAKNGGNGWGFVPDVFLAYLAERGVDKTILKKLC
                     IDNPGRLLTA"
     gene            complement(2349..3314)
                     /locus_tag="PN977_004947"
     CDS             complement(2349..3314)
                     /locus_tag="PN977_004947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HDI6044865.1"
                     /translation="MKKNKYSTPLWMLATILAGMLSPMQSAVNGQLGHWLQDGNACAV
                     ISFASGLVVMFFIIMARKETRQQFAAIPSLIKNRKVPLWNWFAGLCGAMVVFSEGASA
                     SALGVATFQTALISALLLSGLLCDRFGIGVDEKKYFTPYRIIGALFAVIATIFVVSPQ
                     WHSTSFILLAILPFLAGLLAGWQPAGNAKVAEATGSMLVSITWNFIVGFCVLGTALAI
                     RVALGHVTVQLPDVWWMYLGGPLGLMSIGLMAILVRGLGLLMLGVASTAGQLLGSVLI
                     DVLIPSLGNTVYLVTIIGTLFALVGAIVTTIPEYRASKTMKKMEV"
     gene            complement(3371..4129)
                     /locus_tag="PN977_004948"
     CDS             complement(3371..4129)
                     /locus_tag="PN977_004948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytoplasmic protein"
                     /protein_id="HDI6044866.1"
                     /translation="MNNTKKSLKVLFIGESWHIHMIHSKGYDSFTSSKYEEGATWLLQ
                     CLKNSQVDVTYMPAHTVQIAFPEDVAQLEQYDAIVISDIGSNTFLLQNDTFYQLRIKP
                     NALELIKEYVNNGGGLLMIGGYLSFMGIEAKANYKNTVLADVLPVTMLDGDDRVEKPE
                     GVIAQPSQPDHPVIKGFSEYPFFLGYNRAIAKENAEVVLTINNDPLLVFGNYHNGKIA
                     CFMSDCSPHWGTQQFMSWPFYTALWVNILTHIAR"
     gene            complement(4143..5357)
                     /locus_tag="PN977_004949"
     CDS             complement(4143..5357)
                     /locus_tag="PN977_004949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462448.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
                     /protein_id="HDI6044867.1"
                     /translation="MKFERHHEILKRLSKFGSVKVSDLSNSLNVTKETIRSDLNELAR
                     LGYLTRCHGGAFIVLDSLDTIAKNEIAYALENYDTAQGIKKGHSTMKSQVCVIGSFNV
                     DIISYLPRLPTIGESLLASNFIFSPGGKGCNQALAASFADTDVYFITKVGTDHFSDYA
                     INFMNSSKIYKSIIYQTKETQTGTATILVNEGTGDNVIAIYPGANMTMSSDEITIQKE
                     AIINSDVILLQLETNYTALQQAITLAQKNSIPVIINPAPYNDIVNELIQDVDYITPNE
                     TEAGLLSGIDVHDLESAKRAAEAIHNKGVKNTVITLGSKGSLAFDGKKFIHSPAFPAV
                     VKNTAGAGDAFNGALASGLAKGKSLESALCYASAFASLAVETSNASDMPEHESVIHRI
                     QSIHYQQTIFTH"
     gene            complement(5496..5603)
                     /locus_tag="PN977_004950"
                     /pseudo
     CDS             complement(5496..5603)
                     /locus_tag="PN977_004950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080164.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
BASE COUNT         1553 a         1246 c         1293 g         1511 t
ORIGIN      
        1 aatattggtg atgagcctga acgaatgatg tacagacata tgcctctgat acgtcttgtt
       61 gtcagagagt tatcactgaa gaaatcacct gagccagaaa tatctgtgcc tgtagcatcg
      121 ctccgtcgtc tggaacgcct taaacagcac atccgcaaaa aacggcatca gcgttggaca
      181 gaggttatgg ccctgcataa caagggatgt agtttcaggg aaatatcccg tattacaggc
      241 ctgtcgcgag tgacagtcag tcgctgggtg ggttcaggaa cattccctga aatgtcaacc
      301 aggcctccaa agcgagggct tctggaccca tggagggagt ggttaaaaga gcaacgagaa
      361 tgtggtaatt ataactccgg ccggatatgg cgggaaatgg tggccagggg ggttacaggc
      421 agtgaaacca tcgtcaggga tgctgttgcc aaatggcata aaggctggat cccaccggtt
      481 actactgccg caagacttcc ttcagtgtcc cgggtaagcc gctggttgat gccctggaga
      541 ataatcaggg gtgaagaaaa ttatgctttc cgatttatta gtctgatgtg tgaaaaagaa
      601 ccggagttga aaatagcgca gcaactggta ctcgagttct accgtattct gaaaacctaa
      661 aataaatcac agctaagcag ctggttcact cgggtccacg aaagcggctc agcagaactt
      721 cggtgcgtag ctgcggggat ggaagctgat gctgcggcta tatgtgaggc aatcagcagt
      781 cgctggagta ctggtgttgt cgaaggtcat gtaaatcgcc tgaaggtgct gatacgccag
      841 atgtatggtc gagccggact tgaactactt aggcggaggg taatgagtcc actggcatga
      901 aaatcttcac ctaggttgag gaagagccac ttttgcactg ttcgtgacag tatggccttt
      961 atgtactcac tttgttaccg gtagctggat catcactacc gtgccgcaaa tggtggtgaa
     1021 cagcatcaat tgtggaatga atgttgccga agcgaccaat gcgcctcgtt tccaccatca
     1081 gtggttgccg gacgagctgc gtgttgaaaa gggtttagtc cggatacgct caagctgctg
     1141 gaagcaaaag gtcagaaagt ggcgctgaat gaggcgatgg gcagcacgca aagcattatg
     1201 gttgggtcgg atggtgagtt gtacggcgca tccgactcgc gcttggtgga tgatttaacg
     1261 gcggggtatt aattgacatc ccccggcgca atgtgccggg ggataatggt tttatgcggt
     1321 caataatcgt ccgggattat cgatacagag ttttttgagg atcgttttat cgacgccgcg
     1381 ctccgccaga taggccagaa aaacatctgg aacaaatccc cagccattac cgccattttt
     1441 agcccacatt tgtttaagga agacatcgtg gctgagaaca agctgatcgg cgtatcccaa
     1501 ctcaatgaga tgagcgacgg catcggcagt ctcctgcacc cctggcgcta ttccctcttt
     1561 cgggaaggta atgtctaggc caatcatgtc gaattccagc cagacacctt tatcaagcat
     1621 tttccgctga tacgccacgt cttttcctga cggatccgag tgtgcgagag agactttatt
     1681 tggcgagacg cccatttcac ctaacacaat gtcgagtact tcatcaccgc gacgaagcca
     1741 gcccggcatg tgaatattca tcgccacatg aggattgtta atctgtgcca gcgaggcagc
     1801 ccgcaagctg ttatgctcgg cttcggtaaa tgtcggtgag acacctattt caccgatcat
     1861 tccggcacga atatccgtat cgccaatccc ttgattcaat tctttatcga tcgttgtcgc
     1921 tagttcatca accgttttat gaattctctg gctttcaaat ttttccaggt agggaccgga
     1981 ggaagcaaca atattcagac ctgtttttaa tgcgacttct cgtaatgcct gcgcatctcg
     2041 tccgatagac tcagaccctg tggcatcaac aatggtgcga ccaccgagcg agataaagtt
     2101 attaatttcg aagatcacat cttcaatagg tttgcggtcc atattatcag cgcagcaata
     2161 aggatcgtgt tttaatgccc actggatttc tgccgtgact tttttatcaa caagccgttg
     2221 tgaaaaagga tagcaaggag catccacaac agaggaaaga tcgttgaaca gatgttcatg
     2281 aggaagtgtt agccccatat cttcacgggc gacggggccc gtcacagttt gtagataatc
     2341 tttcatgatt atacctccat tttcttcatt gttttcgagg ctctgtattc cggaatagtg
     2401 gtaacaattg caccgaccag tgcaaacagt gttccgatga tcgtgaccag ataaacggta
     2461 ttgcccagcg atggaattaa tacgtcaatc aatactgatc ccagtaactg gcccgccgtt
     2521 gaagcaaccc ccagcatcag taacccaaga cctctgacca aaatggccat taaaccgata
     2581 gacattagtc caagcgggcc gccaagatac atccaccaga catctggtaa ttgaacagtg
     2641 acgtgcccta atgcgacacg aatagctagc gctgtgccta aaacacaaaa tccaacgatg
     2701 aaattccagg tgattgaaac cagcatggat cccgtagctt cagctacttt tgcatttcca
     2761 gcgggctgcc atcctgcaag caaaccagct aagaacggaa gaatggcaag taaaatgaaa
     2821 gaggtagaat gccactgcgg tgacacaaca aaaatcgtag caataaccgc aaataatgca
     2881 ccgatgatgc gataaggggt aaagtatttt ttctcgtcga caccgatacc aaagcgatcg
     2941 caaagtaaac cagacagcaa tagcgcggag atcagtgcgg tctgaaacgt tgcgaccccg
     3001 agtgcgctgg ctgatgcgcc ttcagaaaat accaccatcg ctccacagag accggcaaac
     3061 cagttccaga gaggaacttt tcggttttta atcagagaag gaattgccgc aaattgttgc
     3121 cgggtttctt ttcgcgccat aataataaaa aacatcacga ctaaaccact ggcaaaggag
     3181 ataactgcgc atgcattacc gtcctgtaac cagtgaccta attgaccatt caccgctgat
     3241 tgcatagggg agagcatacc cgcaagaatc gtggcaagca tccataaggg agttgagtac
     3301 ttattctttt tcatacatca atccttctat tatagtaaat ggagtaactg gtgttacccc
     3361 attattatta ttatcttgca atatgagtca gtatatttac ccacaacgcg gtatagaacg
     3421 gccaggacat aaactgctgt gtaccccagt gcggtgaaca gtcactcata aagcaggcta
     3481 ttttcccgtt gtgatagtta ccaaagacca gtaatggatc gttgtttatt gttaatacca
     3541 cctcagcatt ttctttagcg atcgcccggt tataacctag gaaaaaagga tattcagaga
     3601 accctttaat aaccggatgg tcaggttgtg atggctgtgc aataacaccc tctggttttt
     3661 cgacacgatc gtcaccatct aacatcgtaa ccgggagcac atcagccaga actgtatttt
     3721 tataattggc ttttgcttca attcccatga atgagagata gccgccaatc atcaataacc
     3781 caccaccgtt attcacatat tctttaatca gttcgagagc gtttggtttt atgcgaagct
     3841 gataaaaagt atcattttgt agcaggaaag tattactacc aatatcacta ataacgatgg
     3901 catcatattg ttctaactgt gccacatctt cagggaaggc aatttgtacc gtatgtgccg
     3961 gcatataggt gacatctacc tgactatttt tcagacattg gagtaaccag gtcgcacctt
     4021 cttcatattt acttgaggta aaactgtcat atccttttga atgaatcata tgaatatgcc
     4081 aggattctcc aataaagagc acctttagtg attttttggt gttattcatt ctctgtccct
     4141 tcttaatgcg taaaaatagt ttgctgataa tgtatactct gaattcgatg tatcacagat
     4201 tcatgttcag gcatgtcgga agcattgctt gtctccaccg ccagggatgc aaaggcactg
     4261 gcataacaca gtgctgattc cagtgatttt cctttcgcaa gtcctgatgc cagagcgccg
     4321 ttaaaagcat ccccggctcc tgccgtattt ttaacgaccg ctggaaacgc tggcgaatga
     4381 atgaattttt tgccatcaaa tgcgagtgag cccttgctac cgagcgtgat aaccgtgttt
     4441 tttacccctt tattgtggat agcttctgca gctcgtttag ctgattcaag atcgtgtacg
     4501 tctatgccgg acagtaaacc agcttctgtt tcattggggg tgatataatc aacatcctga
     4561 ataagttcgt tgacgatatc attatagggt gccgggttaa taatgaccgg gatactgttt
     4621 ttttgcgcca atgtgatcgc ttgttgcaga gcggtataat ttgtttcgag ctgcagaagt
     4681 atgacatcag aattaataat cgcttctttc tgaatagtta tttcatcgga cgacattgtc
     4741 atgttggcac cgggatagat cgcaataaca ttatcgccag taccttcatt aacaaggatc
     4801 gtggcagttc cggtttgcgt ttctttcgtt tgataaatta tacttttata aatcttagac
     4861 gaattcataa agttgatcgc ataatcacta aagtgatctg tacctacttt tgttatgaaa
     4921 taaacatcag tatcggcaaa gcttgctgcc agcgcttggt tacaaccttt tccccccggt
     4981 gaaaatataa aattacttgc cagaagcgat tctccgatag tgggaagcct cggcaaataa
     5041 ctgatgatat caacattaaa cgaaccaata acgcatactt gacttttcat agttgagtgg
     5101 ccttttttta ttccctgggc tgtatcataa ttttcaagcg cataagcgat ttcatttttc
     5161 gcaatagtat caagcgagtc taggacgata aaagcaccgc catgacatcg agtcagatag
     5221 ccaagtctgg caagttcatt aagatctgat cgaatagttt ctttcgttac attgaggcta
     5281 ttggacagat cggatacttt tacagaaccg aatttactga gtcgtttcaa tatttcatga
     5341 tggcgttcaa atttcatgct tcacctcact tcagttacgt taatatctgt cgatgtatat
     5401 tgatgtgata atcaccataa agtggcgggg aaaagcaaaa aaaatcaaaa atagccggag
     5461 aaaaggtaag cgttaatgga gaatcatatt gatgcttatt tattggtgag aactacgttc
     5521 catggcagga gttcatcaat acggttggaa ggttattccg gcagtacgct caggatatgg
     5581 cgcagatact cttccggatc aat
//
LOCUS       DAMZFT010000229         5591 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.229, whole genome shotgun sequence.
ACCESSION   DAMZFT010000229 DAMZFT010000000
VERSION     DAMZFT010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5591)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5591)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5591
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.229"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(819..1520)
                     /locus_tag="PN977_004951"
     CDS             complement(819..1520)
                     /locus_tag="PN977_004951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001281399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HDI6044868.1"
                     /translation="MSDLIAYKSNALVEASYKLTLQEQRFLLLCIGRLKSGSDAESTE
                     LQKTMTVTAEEYFDSFPDMGRKNAEVQLQEAIDRLWDRSIILKDDEKREEFRWIQYRA
                     QYAKGEGKAQITFSDAVMPYLTQLKGQFTRVVIKNISNLSRSYSIRIYELLQQFRSTG
                     ERIIALDDFRSMLDIEHKYQTYKSLNQQLLRPCVDELNQKSDLAVTLETIKKGRTVVA
                     LHFRFKEDKQIKMTI"
     gene            complement(2053..2343)
                     /locus_tag="PN977_004952"
     CDS             complement(2053..2343)
                     /locus_tag="PN977_004952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001514113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein"
                     /protein_id="HDI6044869.1"
                     /translation="MSRTLEQKIADAEARLQRLKAKSRSLDTAQKVVVGAALLAKVRK
                     PEEVQLRAWLLQFLKAEVTRQADVSRIQPLIDELNALPKPVPKEVSENDQQA"
     gene            2522..4681
                     /locus_tag="PN977_004953"
     CDS             2522..4681
                     /locus_tag="PN977_004953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="HDI6044870.1"
                     /translation="MAIYHLSMKIISRSSGYSAVASAAYRSGSLMLDERTGLTHDYTR
                     KSGVAEAVILTPATAPAWCTNRAELWNAVEKAERRKNSQLAREIELAIPRELQQDAAR
                     ETVLAFVRENFVSQGMIADVAFHHMDKTNPHAHIMLTTRAVGPAGFGGKVRDWNDRTH
                     AETWRASWADHANRALANAGYQEEIDHRSYERQGLEKTPGIHLGKSACAMETRGIETE
                     RGEQNRLINRLNLEIQISRTELRNSGLTEPARRVADLLNIVLPDNATSYTLRDIIEAL
                     PKDSNAAWKLTSNAMSMMADMQATRRRWEELNTERKEAMSHAASLKKSSPFSSGFSRI
                     PLMQWAAPEYRSEQGKIQSLSQQMEKLRQHYRKVEKQDIPASQTAFETQWQQWGASGL
                     AALREQLRKREEELRRKEQEETERRRAERRAEQLRKNDNAVMDSGVLSAVVTGYGEAP
                     MPGNGEMTCYLLLHNRNGEYTLWGNELEKYRTRIFECVDLMRDRSGYICDRSEIVQHP
                     PLQRVYSSATFEQLLTQVCQTWPQYTRNLRQPKTWPESFCLGEDRQPAIPSPAARKVD
                     FMQGRLPPTLMPVMSSVDRETRQLQLLLVMGVDDSLGGVVRLNGTLYPAFAVPSADNR
                     HLVISALTDKGLRYAGYGVAVNYDADSQISPAPELMKFHLKTREAPLFAAVNTPEKQP
                     DHLFRSLGFNRTWDEWRRDEQHRKQERYPGNHRGMSM"
BASE COUNT         1526 a         1325 c         1286 g         1454 t
ORIGIN      
        1 aaaaaaaaac cctcaatgaa tgagggtaag tgtaaggaag tacctgattt attttacata
       61 ttgaccatca ataactgagc aaaaaaaata acaaaaccat gacatagata caacctattt
      121 ttcctgtgca agattacctc gtttgccata aaactccgtt cttcagctca aggaaaattt
      181 tgaaaaccat agcttatatc actatatgaa tgaatatatt gttgccatat tgcagtgtaa
      241 tatttattat tttttgattg tttttagttt attattttat tttttttcta tatttccaaa
      301 gataatcttt ttatattctg tttctcgata ctagacataa aatcaaattc acgataaata
      361 ttaattgcgc gtttctgccc cccccccaca cctactaatt actacctgtg tttagttaca
      421 caatcagaaa cgcgcttttt aagcgttaaa atagcgtgat gaactcctac agatattcaa
      481 atacctctac gtcatcgtga aaggacgcgc tttgccagag gcgaggtttt gttttcaagt
      541 gtgccctggt gcttgatatc catcccgtcc tgaaggttgg agattcctac gttgctcagg
      601 cttgctcttt agtcacgcat gagtcacttc gatgggggac tgctgctggc ggcattaacg
      661 catctctcac gtcaagtaaa acaagtaatg ttcgacatgg tttgccatac cgctctctaa
      721 aatcgatttt gacgcaattt atttgcttgg tagtaccatc gcatacctga caccaaaaaa
      781 acgaaccaga tggcgtttta gatgccttaa aatcgatttt agatcgtcat cttgatctgt
      841 ttgtcttcct tgaagcgaaa atgcagggct acaaccgtgc gccccttctt gatcgtctca
      901 agcgttacag ccaagtcgct tttttgattc agttcgtcaa cgcaaggtct taataattgc
      961 tggttcagag acttatatgt ttgatattta tgctctatat caagcattga gcgaaaatca
     1021 tcgagggcga taatacgttc ccctgtgctg cggaattgct ggagaagttc gtatattctt
     1081 atgctgtaac ttcttgataa attggagata ttcttaatta caactctagt aaattgcccc
     1141 ttcaactgtg tcagataagg catgaccgca tctgaaaaag tgatttgggc tttgccctca
     1201 cctttagcgt actgtgcccg atactggatc cagcggaact cctcgcgctt ctcatcgtcc
     1261 ttaaggatga tcgacctgtc ccacaaacga tcaattgctt cctgtagctg aacctcggca
     1321 tttttccgcc ccatatcagg gaatgagtcg aaatactctt cggcggtaac agtcatggtc
     1381 ttttgcaatt cagtggattc tgcatcagaa cccgacttca atcgaccaat gcaaagtaac
     1441 aagaatcgct gttcctgaag agtcaattta tagctggcct caaccagtgc attggatttg
     1501 taggcaatca gatccgacat tgtaaatccc tcaacctaag gcaatgagta aaatataaac
     1561 attcccttag gtaaaggcaa tatgatttaa ttttaatatc ccttttcctg tggatattac
     1621 cttaaatcat attgccttta ccttaaatca tattgcctat ccaccttaaa tcatattgcc
     1681 tttaccttaa attatattac ctatagtctt ctaaactatt gaattccata agaaaattcg
     1741 accctaaaac aagtattaaa aacaagatct taaaaacaag atctaaaaac ttaacagcga
     1801 gcctgtggaa aacttggggg gcgtaaaagg aaaaaaaaag caaaaacaac atgttacaaa
     1861 aaacaggttc gcggacttta gtaaaactcc cgctcaaccc tgtggaaatc cagtattcat
     1921 gcgggttaag cgcatatcac ctgtcaaaaa accgtgcgta tgtgctcaac ctggctacgg
     1981 ttcacttccg cttcctcgcc cactcgctcg ctaacgctcg gtcgctgggg ctgcggcgag
     2041 cggacagtgc attcacgcct gttggtcgtt ttctgacact tctttgggga ctggttttgg
     2101 caacgcgttc agttcgtcaa tcaacggctg tatcctgctc acatcagcct gccgtgttac
     2161 ctctgccttc aggaactgga gtaaccaggc tcgcaactgc acttcctccg gctttctgac
     2221 ctttgccagc aatgctgcac ctacaacaac cttctgcgct gtgtccaggc tccggctctt
     2281 cgccttcagg cgctgtaatc tcgcctcagc gtcagcaatc ttctgttcga gcgttctgct
     2341 catgatgtaa ctccgtgcgc gatgaaaaat cgcattttag cgcgtcactg gtagtttaaa
     2401 aactgagctg gcataatgca cggcacatca cgaagtgcgc acttatacaa tcttcactcc
     2461 gtttcgattg tgtgcgccct gcggggctga aagaaaacta caaaaaggca ttacggcaga
     2521 aatggcgatt tatcatctca gcatgaaaat tatctccaga agcagcggct acagcgccgt
     2581 agcttccgct gcctatcgtt ccggttcact gatgcttgat gagcgtaccg gactgactca
     2641 cgactacacc cgcaaaagcg gtgtggctga agctgtgatt ctgacccccg cgacagcccc
     2701 tgcctggtgt acgaaccgtg ccgaactctg gaatgccgtt gagaaggccg aacgccggaa
     2761 gaactcacaa cttgcgcggg aaattgaact cgctatcccc cgtgaactgc agcaggatgc
     2821 cgcacgggaa accgttctcg cctttgtcag agaaaatttt gtcagtcagg gcatgattgc
     2881 tgatgtggcg ttccatcaca tggataaaac aaatccccat gcgcacatca tgctgaccac
     2941 gagggctgtc ggacctgctg ggttcggcgg aaaggtcagg gactggaacg accggacaca
     3001 tgccgagacg tggcgtgcat catgggctga ccatgcaaac agagcactgg cgaacgctgg
     3061 ttatcaggaa gagatagacc atcgttcata cgagcgacag gggcttgaga aaacgcctgg
     3121 tattcacctc ggaaagtctg cctgtgcgat ggaaacacgc ggtatcgaaa cagaacgcgg
     3181 cgaacagaat cgtctgatta acaggcttaa cctggaaata cagatttccc gcactgaact
     3241 caggaatagc gggctgacag agcctgcccg ccgtgtggct gaccttctga atatcgtgct
     3301 tcctgacaac gcaacttcgt acacactacg tgacatcatc gaagcgttgc cgaaggacag
     3361 taacgcggca tggaaactca ccagtaacgc catgtcgatg atggcagata tgcaggcgac
     3421 gcgcagacgc tgggaagagc tgaacaccga acgaaaggaa gcgatgagcc atgctgccag
     3481 cctgaaaaaa tcatcgccgt tttcgtccgg tttttcccgt atacccctga tgcaatgggc
     3541 tgcaccggaa taccgtagtg agcagggaaa aatccagtct ctgagccagc aaatggaaaa
     3601 attgcgacag cattaccgga aggtggagaa acaggacatt cccgcgagtc agacggcatt
     3661 tgaaacacag tggcagcagt ggggcgcatc cggccttgca gctctcagag agcagttgcg
     3721 taaacgtgaa gaggaattgc gccggaagga gcaggaggaa acagaacgtc gtcgcgcgga
     3781 gcgacgggcg gaacagctca ggaaaaatga caatgccgtt atggattcag gggtactgag
     3841 cgcagtcgtt acggggtatg gcgaggcccc catgcctggt aacggggaaa tgacctgtta
     3901 ccttctgctt cacaaccgga acggagaata tacgctctgg gggaacgagc tggagaaata
     3961 ccgcaccaga atctttgagt gtgttgattt aatgcgtgac aggagtggtt atatctgtga
     4021 ccgaagcgaa atcgtccaac atccaccact gcaacgtgtt tattcctctg cgacctttga
     4081 gcaacttctg acgcaggttt gccagacatg gccgcaatat acccgcaatc tccgccagcc
     4141 gaagacatgg ccggagtctt tctgtcttgg agaagaccgc cagccagcca taccatcacc
     4201 agcggcaagg aaggtggatt ttatgcaggg acgtctgcca ccgacgctga tgccggtcat
     4261 gagttctgtc gaccgggaaa cccgccagct tcagcttttg ctggtcatgg gcgtggatga
     4321 ctctctgggc ggtgttgtcc ggcttaatgg tacgctgtat cccgcgtttg ccgttccctc
     4381 agccgataac aggcatctgg tcatcagtgc gctgaccgac aaggggttac ggtatgcagg
     4441 gtatggtgtt gccgtcaatt acgatgcaga cagccagata tcacctgcac cggaacttat
     4501 gaagtttcat ctgaaaaccc gtgaggcacc gctttttgct gccgtcaata caccagaaaa
     4561 acagccggat catctgttcc gctctctggg ttttaatcgt acctgggacg aatggcggcg
     4621 cgatgaacag cacagaaaac aggaaagata tcctggaaac cacaggggaa tgagcatgta
     4681 ggtatctttg gtgttatccg gatcgccgta aaccctgcca tgaaggacag attttgacgg
     4741 cgatcaggat aaaaaatgcc ataatgattg tggccttaaa attatcgaaa acacgaattt
     4801 agttttacat ttgtgcgcgt atgtacgcct caaaaaaaca accaacgaaa aacaactcat
     4861 caacaaaata ataacaaaac cttcttgttt tgtgatttat gttgcacagt ttgattgtaa
     4921 gtcacatttg tgaaacgaaa ttgttttgcg ctatggcatc tgatgagata aaagaaagag
     4981 gggtgtttct gtatcgtaaa gtctctagcc ggatagcttt tcattggtag tacagaaaca
     5041 tccccactca tcaggattca ggatcgatag ccattcgata taagactgct gattgtgtct
     5101 tcgttaaaat ctatcttttc acgtgagaat taagaactgt taattcatcg tttgtattcc
     5161 ctatcattag gataggggat atttttatcg caaaatacac accaaatgta gtgttatata
     5221 agttttcaat gaccgtttct tactgaaaag aagatagggg attcgtgttg acatcctcca
     5281 tgccctgaag gacggggttt tacggcgcac cgagtaaatc ttccccccga aagcccagta
     5341 tcggggggaa gatgcattaa cggttcgtct tcggatacta ccgtgattgc ggtgatgagc
     5401 atatcagctc aacgggagaa attgaacgcg tttctgacag tgtggctaac acggcgaaat
     5461 agacctcaga tatctgccag aaacgcctca cgtattttta acgtgtatca atatccatgt
     5521 cgagatagtt atccggtgtg ctgtaaaacc ccgtccttca gggcatggag gatgtcaact
     5581 tagatatgga t
//
LOCUS       DAMZFT010000230         5583 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.230, whole genome shotgun sequence.
ACCESSION   DAMZFT010000230 DAMZFT010000000
VERSION     DAMZFT010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5583)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5583)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5583
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.230"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(222..1058)
                     /locus_tag="PN977_004954"
     CDS             complement(222..1058)
                     /locus_tag="PN977_004954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001189838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDI6044871.1"
                     /translation="MQRSWFNNRLTSAKQKSLLYKSLADLVQSMMDTFVDPWLERITN
                     RKSIFSMSKEDLETRTNELGQFFTIRTSNSSSVPMLLQQRFDEIHFKGTERPINQTIY
                     REFNGISVLWDPIYAPVDFERHPYGTVLIPESTLETTGGTFGEMFLTSRGMISIPIND
                     LARTMGITGTIDQSAITEEILRKFNQFVKPLLPLHIVFDGLTLYLSVVVNEQADMITL
                     NEISDTEKAFCWFETSDTTSLTEVTSINAPITATPGGTIVKATPTFDRTRADDLLLDS
                     DA"
     gene            complement(1058..2491)
                     /locus_tag="PN977_004955"
     CDS             complement(1058..2491)
                     /locus_tag="PN977_004955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000047570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bleomycin hydrolase"
                     /protein_id="HDI6044872.1"
                     /translation="MSKTTPTKDSIRAEFEELVEKDSFWSKFVGSQFVSMLTLFITQI
                     VYRCFQYADAALAEGFISTATRRSSILAAAETNSYVGTKPTPSSGMVEITATSEDAPA
                     VIPKNTPLISDDQYPYMTMDVCRLVDGTGTVEVAQLEIQEVTYTVTAAKEFLEVVLSK
                     ALTAVCYKLEVFVTTDGKTTQWSSSTMFRLAGSKSQVYVEFYKPSEQLGVRFGDGLIG
                     QIPPEGSTITLKVWCTNGDITLVAGQNLTPVDSAANLANLISVKTTTPITAGTDAETT
                     EITRNRAQYYLAYDDQVVWGGDYTYFLVRNIPGLSWVKAWGEGQQEKLDGAYNVRNIN
                     KIFISGWHPNKSQSELEEMILAAFKKVPNELNKKFSYKEVRKLPFKITITGRISASLT
                     IENVTDELKSALETKFGRDSTFFDPNRVGKYILIKKKDVWAFIETLGYFRDFYLEFVE
                     WNESNGFYDFVYLDTENSTFNISYEEE"
     gene            complement(2488..2844)
                     /locus_tag="PN977_004956"
     CDS             complement(2488..2844)
                     /locus_tag="PN977_004956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000002798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6044873.1"
                     /translation="MANNNEIDPLLTLELSGVKTYESQEEAWGARLYEWLNTYQGEVY
                     GDPSWGNVLPQFKHEPTNLSHVQIAVEAMLLQKLTVDLPDIPISGLSVAEGDAFDKLK
                     ISIRIRDITITQDVVL"
     gene            complement(2844..>5583)
                     /locus_tag="PN977_004957"
     CDS             complement(2844..>5583)
                     /locus_tag="PN977_004957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000440161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HDI6044874.1"
                     /translation="AGPLWAAGKELGGISKEVGGSLNGARKSIADVIRGNDDNSRRKG
                     FFRRKSQNSADVVQVNTQKRTVQELQDQTSEIKEGNDKILSALDQIAKNTGKKKGGLL
                     SKLFSLLGKGAGGVASLLMGRGMLKKAGALAFGALGAKKLVGMLRGGGKKTLAHEGGD
                     LAARAAGKLGLKAVGKGALRAIPLVGTVAGGIYDAVTGWNDTEAQRRAFGLKSGQDPS
                     FQQKAAYTLANVLDLGGLVSGISSAIGDVLKSLGFEDIGNMLQSFSTESIAQAIDSGI
                     TNLETYISNLVDTISTKFDDYTAKIGDAVSAWFSDTSNKLLEKLDAIKDFFTVDNLKQ
                     VFSDAIDSAIDFIKNPGKHIKEAASNIWDGVKNLPGKALDAAVDAVKNTPAAMIVSKI
                     PNPIGEANAKEITPELKAPVNSQQGTSDSKAESDAKQTNIAARVINAALDMAKDSNKT
                     VKETANQIINANAVETGNSALQKIDKAIGQNSSSSSSLNTTGTRNDIQKAADTYNNGN
                     LDVKVGSLGAEGKANLDKLAPYFAELENKYGLPEGTLYAIAATESGGNPYAKSQTGAL
                     GMFQFTGIAREETGLAEGESFDPVKSAEAAALLMSKYLKQANGDLNEAITAYNAGFGT
                     INKWKKGTGDLSKENREYAIKVNTHRARYLGGEIYTPGAGAQGGAQYGVRGPLPDNAV
                     IDQSTGLAFTPGDSPFEKGGLVDKIGNAVGVNDLVNKFMNGRGMRREVVQGTLEERAR
                     GKGTATAAGNVYVDTPMPVEEARPVANNSSYFDQLGAQMGIDGLFDKLRNSPGMRKNN
                     APEPASTSQVTIAANDLQQPTGRMQIDGQVISDLGGSGAKPTMQLADNTVSLDGETKR
                     LFAQMTSLLARIEEHTKDSAKGQGTVVKVSTPQPGVMRTVPLSIDDPLMNDYARVD"
BASE COUNT         1399 a         1438 c         1177 g         1569 t
ORIGIN      
        1 ttcttagtaa ccagtgaact ggtaccgaaa acggccttgc tgattaaaaa tcgagagcct
       61 ttgttaatgg acgattcagc gcgccgctga tagtaatagt ccaacaactg actcttatag
      121 aggtttgttg agacgtcaga catgattttc cctaatcaat gttgtgaagc ctcattgtaa
      181 gataagtaac ttgtcacccc gccctgcgga cggggtgatt gtcacgcatc gctatccaac
      241 agcaaatcat ctgcgcgagt gcgatcaaac gtaggcgttg ctttcacaat agtgccgccc
      301 ggcgttgcag tgatcggggc gttaatcgac gtaacttcag taagcgaagt tgtatccgaa
      361 gtttcaaacc agcagaatgc tttttcggta tcagaaatct cattcaaagt gatcatgtcg
      421 gcctgttcat ttacaacaac cgacaaatag agcgtaagcc catcaaacac tatatgcagt
      481 ggcagtagag gctttacgaa ctgattaaac tttctgagaa tttcttctgt aattgcggac
      541 tgatctatcg ttccagtaat acccattgtc cgggccaggt cgtttatggg aatactgatc
      601 attcctctgg aagtcagaaa catctcgccg aatgtgccgc cggtagtttc cagtgtgctt
      661 tctggaatca ggaccgtgcc atagggatga cgttcaaagt ccaccggagc atatatggga
      721 tcccataaaa ccgatatacc gttaaattcg cgataaattg tctggtttat agggcgttca
      781 gtacccttaa aatggatctc atcaaaccgc tgttgtaaca acatcggaac ggaagatgaa
      841 ttcgacgttc tgatagtaaa aaactggcca agttcatttg tcctagtctc cagatcctcc
      901 ttgctcatgg agaaaataga cttccggttg gtaattcgct ccaaccatgg gtcaacaaag
      961 gtgtccatca ttgactgaac caaatcagcc aatgatttat aaagtaatga cttttgctta
     1021 gctgatgtaa gccggttatt aaaccaggaa cgctgcatca ctcctcctca tacgaaatat
     1081 taaaggtgga gttttctgta tccagataaa cgaaatcgta aaagccgttg gactcattcc
     1141 actcgacaaa ttccagataa aagtcgcgga aataacccag cgtttcgata aatgcccaaa
     1201 cgtctttttt cttgattagg atgtacttgc cgacacggtt cggatcaaag aaagttgagt
     1261 cacgcccaaa ttttgtttcc agtgccgact tcagctcatc agtcacgttc tcaatggtca
     1321 ggcttgccga tatccgccca gtgatggtga tcttaaaggg tagttttctg acctctttat
     1381 acgagaattt cttgttcaac tcattcggca ccttcttaaa ggcagccagg atcatttctt
     1441 caagctctga ctggcttttg tttggatgcc atcctgaaat aaatatctta ttgatattcc
     1501 gaacattata agcaccatct aatttctctt gctggccttc tccccatgcc tttacccagg
     1561 acagtcccgg gatgttacgc accagaaaat acgtatagtc cccgccccat acgacctgat
     1621 catcataggc aaggtaatat tgtgcacgat tacgtgtgat ctccgttgtt tcggcatcgg
     1681 tacctgcggt tatgggtgtc gttgtcttaa ctgaaatcaa attagctaaa ttagccgcag
     1741 aatcgacagg agtcaggttt tggccagcaa ccagggttat atctccgttg gtgcaccata
     1801 ccttaagtgt aatggtcgag ccttctggcg gtatttgccc aattagccca tcaccgaatc
     1861 gaacccccaa ctgctcggat ggcttataaa actcaacgta gacctggctt ttactaccgg
     1921 ctaaccggaa catagtgctg gaagaccact gcgtggtctt accatcggtc gtcacgaata
     1981 cttccagctt atagcagaca gcagtgagag cctttgataa cacgacttcc agaaattctt
     2041 tggctgccgt aacggtatat gtcacctcct ggatttccaa ctgtgccact tctaccgtac
     2101 cggtgccgtc aaccaacctg catacatcca tagtcatgta agggtactgg tcgtcagata
     2161 ttaaaggcgt gtttttgggg attaccgctg gagcatcttc acttgtggct gtgatctcaa
     2221 ccatcccgga tgacggtgtt ggcttggtac caacgtaact attcgtttct gccgctgcca
     2281 ggatagagga acgccgcgtc gcggtcgata taaagccttc agccagcgcc gcatcggcat
     2341 actgaaagca cctgtagaca atctgggtaa taaacaatgt cagcatcgag acaaattgag
     2401 agccgacaaa cttcgaccag aatgaatctt tctcgacaag ctcttcaaac tctgcacgaa
     2461 tactgtcttt agtcggtgtt gttttactca tagcaccacg tcctgtgtga tagttatatc
     2521 cctgatacga atggatattt tcaacttatc aaaagcatct ccctcggcta ctgacaagcc
     2581 agaaatcggt atgtcaggta aatctaccgt cagtttttgc aacagcattg cctcaaccgc
     2641 aatttgaaca tgcgacaagt tggtcggttc gtgtttaaac tgcggtaaaa cattgcccca
     2701 tgacggatct ccgtatacct caccctgata agtgtttagc cactcatata aacgagcgcc
     2761 ccaggcctcc tcctgggact catacgtttt tacgccggat aactccagcg tcagtaaagg
     2821 atcaatttca ttattgttgg ccatcaatca actctcgcgt agtcattcat caacggatca
     2881 tcaattgaca gtggtaccgt gcgcataacg cccggctgag gcgtgctgac ctttacgaca
     2941 gttccctggc ctttcgccga gtctttggtg tgctcttcaa tcctggcaag caatgaggtc
     3001 atctgcgcaa acagccgctt cgtttcacca tcaagtgaaa cggtattatc agccaactgc
     3061 attgtcggct tggcaccgga gccgccaagg tcactaataa cctgtccgtc tatctgcata
     3121 cgaccggttg gttgctgcaa atcgttggcg gcaatcgtca cctgggacgt ggaggctggt
     3181 tcaggcgcat tatttttccg catccccggc gagttgcgga gtttatcgaa tagtccatca
     3241 atccccattt gtgcgccgag ctggtcaaag taacttgagt tgttggccac cggacgcgcc
     3301 tcttcaactg gcatcggggt atcaacatac acattgccag ctgctgttgc ggtccccttc
     3361 cctcgtgcac gttcttcgag cgttccctga acgacttccc gacgcatacc ccggccattc
     3421 atgaatttgt tgaccagatc gttaacgcca acagcattgc caattttgtc taccagaccg
     3481 cctttctcaa acgggctatc accaggggta aacgccaggc cagtagactg atcgataaca
     3541 gcgttatcag gcagtggtcc cctcactcca tattgcgccc caccctgtgc tcctgctcca
     3601 ggtgtataga tttcaccacc taaatagcga gcacgatgag tattgacctt gatcgcgtac
     3661 tcacggtttt ctttcgataa gtcacctgtg ccttttttcc acttattgat agtgccaaac
     3721 ccagcattat atgcagtgat ggcctcgttt aagtctccat tggcttgctt cagatatttg
     3781 ctcatgagaa gagccgcagc ttctgccgat ttcacaggat caaacgattc accttcagct
     3841 aagccagtct cttcacgagc aatccccgtg aactgaaaca tccccagagc accggtttgg
     3901 gattttgcat acggattacc accagattca gttgcagcaa tcgcgtaaag agtgccttct
     3961 ggaagaccgt atttattctc tagttcggca aaatacggag ccaacttatc gagatttgcc
     4021 ttaccttcag cgccaagact tccgactttt acatccaagt tgccattgtt gtaggtatcc
     4081 gcagctttct gaatgtcatt cctggtgcca gtggtattaa gcgacgatga tgacgagcta
     4141 ttttgaccaa tagctttatc aattttctgc aacgcgctat tgcccgtttc tacggcattt
     4201 gcattgataa tctgattagc agtttcttta actgttttat tgctatcttt cgccatatcc
     4261 agggccgcat ttatcacgcg ggcagcaata ttagtctgtt tggcatcgga ttcagcttta
     4321 gaatcagatg tcccctgctg gctattaacc ggagctttta actctggagt gatttctttc
     4381 gcattagcct cgccgatcgg attgggtatt tttgatacaa tcattgccgc aggggtattt
     4441 ttaacggcat caaccgctgc atctaatgct ttacctggta aatttttaac cccatcccaa
     4501 atattactag ctgcctcttt aatgtgtttc cctgggttct taatgaaatc aattgcacta
     4561 tcaattgcat cactgaaaac ctgtttcagg ttatcgacag taaagaagtc tttgatggca
     4621 tccagctttt caagcagctt attagatgta tcgctaaacc atgctgaaac agcatcacca
     4681 atctttgctg tgtaatcatc gaacttggta gaaatggtgt cgacaaggtt agaaatatat
     4741 gtttctaagt tggtaatccc actatcaatg gcctgggcaa tactttccgt cgaaaatgat
     4801 tgcaacatat tgccgatatc ctcaaatcca agtgatttga gaacatcacc aatagcactg
     4861 ctaataccag ataccagtcc ccccaaatca agaacattgg ctaacgtata agcggctttt
     4921 tgctggaatg atggatcttg tcctgattta agcccaaacg ctcgacgttg cgcttctgta
     4981 tcattccaac cggttaccgc atcataaata cctccagcca ctgtgccgac taggggaatt
     5041 gcgcgtaacg cccctttacc aactgccttt aatccaagtt tacctgctgc ccgggcagcc
     5101 aaatctccgc cttcatgggc gagagtcttc ttgccaccac cgcgtagcat tcctacaagt
     5161 ttctttgccc ccagagcgcc aaaagcgagt gctccagctt ttttcagcat gccacgcccc
     5221 attaacaacg acgcgacgcc accggccccc ttccctaaca ggctaaataa tttggacagc
     5281 aagccgccct tctttttccc ggtgtttttg gctatctgat caagggcgct gagaatcttg
     5341 tcattgccct ctttaatttc gctggtctga tcctgaagtt cctgaaccgt ccgtttttgg
     5401 gtgttaacct gaacgacatc ggcactattt tgcgatttac gcctaaaaaa accttttcta
     5461 cggctgttat cgtcattgcc acgaatcaca tcggcaatag actttctggc accattaagc
     5521 gatccaccaa cttcttttga tatcccgcca agctccttcc ctgcggccca caatggacca
     5581 gca
//
LOCUS       DAMZFT010000231         5478 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.231, whole genome shotgun sequence.
ACCESSION   DAMZFT010000231 DAMZFT010000000
VERSION     DAMZFT010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5478)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5478)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5478
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.231"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(<1..2116)
                     /gene="torZ"
                     /locus_tag="PN977_004958"
     CDS             complement(<1..2116)
                     /gene="torZ"
                     /locus_tag="PN977_004958"
                     /EC_number="1.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310609.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine N-oxide reductase TorZ"
                     /protein_id="HDI6044875.1"
                     /translation="MTLTRREFIKHSGIAAGALVVTSAAPLPAWAEEKGGKILTAGRW
                     GAMNVEVKDGKIVSSTGALAKTIPNSLQSTAADQVHTTARIQHPMVRKSYLDNPLQPA
                     KGRGEDIYVQVSWEQALKLIHEQHDRIRKANGPSAIFAGSYGWRSSGVLHKAQTLLQR
                     YMNLAGGYSGHSGDYSTGAAQVIMPHVVGSVEVYEQQTSWPLILENSQVVVLWGMNPL
                     NTLKIAWSSTDEQGLEYFHQLKKSGKPVIAIDPIRSETIEFFGDNATWIAPNMGTDVA
                     LMLGIAHTLMVQGKHDKAFLEKYTTGYPQFEEYLIGKSDNTPKSAAWAAEITGVPEAQ
                     IVKLAELMAANRTMLMAGWGIQRQQYGEQKHWMLVTLAAMLGQIGTPGGGFGFSYHYS
                     NGGNPTRVGGVLPEMSAAIAGQASEAADDGGMTAIPVARIVDALENPGGKYQHNGKEQ
                     TYPNIKMIWWAGGGNFTHHQDTNRLIKAWQKPEMIVVSECYWTAAAKHADIVLPITTS
                     FERNDLTMTGDYSNQHIVPMKQAVAPQFEARNDFDVFADLAELLKPGGKEIYTEGKDE
                     MAWLKFFYDAAQKGARAQRVTMPMFNAFWQQNKLIEMRRSEKNEQYVRYGDFRADPVK
                     NALGTPSGKIEIYSKTLEKFGYKDCPAHPTWLAPDEWKGTADEKQLQLLTAHPAHRLH
                     SQLNYAELRKKYAVADREPITIH"
     gene            complement(2141..3241)
                     /locus_tag="PN977_004959"
     CDS             complement(2141..3241)
                     /locus_tag="PN977_004959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NapC/NirT family cytochrome c"
                     /protein_id="HDI6044876.1"
                     /translation="MRGKKRIGLLFLLIAVVVGGGGLLLAQKALHKTSDTAFCLSCHS
                     MSKPFEEYQGTAHFSNQKGIRAECADCHIPKSGMDYLFAKLKASKDIYHEFVSGKIDS
                     DDKFEAHRQEMAETVWKELKATDSATCRSCHSFDAMDIASQSESAQKMHNKAQKDGET
                     CIDCHKGIAHFPPEIKMDDNAAHELESQAATSVTNGAHIYPFKTSRIGELATVTPGTD
                     LTVVDASGKQPIVRLQGYQMQGSENTLYRAAGQRLALATLSEEGIKALAVNGEWQADE
                     YGNQWRQASLQGALTDPALADRKPLWQYAEKLDDTYCAGCHAPIAADHYTVNTWPSIA
                     KGMGARTSMSENELDILTRYFQYNAKDITEKQ"
     gene            complement(3629..4375)
                     /gene="cutC"
                     /locus_tag="PN977_004960"
     CDS             complement(3629..4375)
                     /gene="cutC"
                     /locus_tag="PN977_004960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HDI6044877.1"
                     /translation="MALLEICCYSMECALTAQQNGADRVELCAAPKEGGLTPSLGVLK
                     SVRQRVTIPVHPIIRPRGGDFCYSDGEFAAILEDVRTVRELGFPGLVTGVLDVDGNVD
                     MPRMEKIMAAAGPLAVTFHRAFDMCANPLNTLNNLAELGITRVLTSGQKSDALQGLSK
                     IMELIAHGDAPIIMAGAGIRAENLHHFLDAGVLEVHSSAGAWQASPMRYRNQGLSMSS
                     DAHADEYSRYVVDGAAVAEMKGIIERHQAK"
     gene            complement(4389..4955)
                     /locus_tag="PN977_004961"
     CDS             complement(4389..4955)
                     /locus_tag="PN977_004961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310612.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDI6044878.1"
                     /translation="MANWQSIDELQDIASDLPRFTHALDELSRRLGLDITPLTADHIS
                     LRCHQNATAERWRRGFEQCGELLSENMINGRPICLFKLHEPVQVAHWQFSIVELPWPG
                     EKRYPHEGWEHIEIVLPGDPETLNARALALLSDEGLSLPGISVKTSSPKGEHERLPNP
                     TLAVTDGKTTIKFHPWSIEEIVASEQSA"
     gene            5171..>5478
                     /locus_tag="PN977_004962"
     CDS             5171..>5478
                     /locus_tag="PN977_004962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HDI6044879.1"
                     /translation="MNIQALLSEKVRQAMIAAGAPADCEPQVRQSAKVQFGDYQANGM
                     MAVAKKLGMAPRQLAEQVLTHLDLNGIASKVEIAGPGFINIFLDPAFLADHVQQALAS
                     "
BASE COUNT         1304 a         1464 c         1254 g         1456 t
ORIGIN      
        1 tgtgaatagt gatcggttca cgatcggcaa ccgcatattt tttacgcagt tccgcatagt
       61 taagctgact gtgtaaacgg tgtgccggat gcgcggtcaa aagctgcaac tgcttctcat
      121 cggcggtacc tttccactca tcaggcgcaa gccaggttgg gtgtgccggg caatctttat
      181 agccaaattt ttccagcgtt ttggagtaaa tctcaatttt gccgcttggc gtacccagcg
      241 catttttcac cggatcggcg cggaaatcac cataacgaac gtactgttcg ttcttctcgc
      301 tgcggcgcat ttcgatcagt ttattttgct gccagaaagc attaaacatc ggcatagtca
      361 cgcgttgcgc acgcgcacct ttctgagcgg catcatagaa aaatttcagc cacgccattt
      421 catctttacc ttcggtatag atctcttttc cgccaggttt gagtaattca gcaagatcgg
      481 caaacacatc aaaatcgtta cgcgcttcaa attgcggagc cacagcctgc ttcatcggca
      541 caatatgctg gttgctgtaa tcaccggtca tcgttaagtc atttcgctca aacgatgtgg
      601 tgatcggtaa tacgatatct gcatgtttag cagctgcggt ccagtagcat tcagaaacga
      661 cgatcatctc cggtttctgc catgctttaa tcagacgatt agtatcctgg tgatgggtaa
      721 agttaccgcc acccgcccac cagatcattt tgatattcgg atacgtttgt tctttaccgt
      781 tatgctgata tttgccgcca ggattttcca gcgcatcgac gatacgggca acaggaatcg
      841 ccgtcattcc gccatcatca gcggcctcgc tggcctgtcc ggcaatcgcc gcagacattt
      901 ccggcagcac gccgccaaca cgcgtcgggt tgccaccatt ggagtaatga taagagaaac
      961 caaaaccgcc gcccggtgta ccaatctgcc ctaacatcgc cgccagcgtc accagcatcc
     1021 agtgtttctg ttcaccatat tgctggcgct gaattcccca gcctgccatc agcattgtgc
     1081 gattcgccgc catcagttca gcgagtttga ctatctgcgc ttccggtacg ccagtaattt
     1141 ccgccgccca ggccgcgctc ttcggcgtat tatcgctctt acctatcaga tactcttcaa
     1201 actgcgggta gccggtggtg tatttttcca ggaaggcctt atcgtgctta ccttgtacca
     1261 tcaaggtgtg cgcaatccct aacatcaacg ccacgtcggt acccatattt ggggcgatcc
     1321 aggtggcgtt atcgccaaag aactcgattg tttcggaacg gatagggtcg atggcaatca
     1381 caggtttgcc cgattttttc agctgatgga agtattccag cccttgttca tcggtactgc
     1441 tccaggcaat ttttaaggta ttcagcgggt tcatccccca cagcaccaca acctggctgt
     1501 tttccagaat cagcggccag gaggtctgct gttcatacac ctctacagaa ccgaccacat
     1561 gcggcatgat cacctgtgct gccccggttg aataatcgcc gctatgcccg gaataaccgc
     1621 ccgccaggtt catataacgt tgcagtaagg tttgcgcttt atgcaacacg ccagaagagc
     1681 gccagccgta agatccggca aaaatggccg atggtccgtt agctttacga atacgatcgt
     1741 gttgctcatg aatcagtttt aatgcttgct cccagctcac ctgtacatag atatcttcgc
     1801 cacgaccttt cgccggttgc agtggattat cgagatagct ttttctcacc atcggatgct
     1861 gaatacgcgc cgtggtgtgt acctgatccg ccgccgtaga ctgtaaggag ttcggtatgg
     1921 ttttcgccag cgcgcctgtc gaagaaacaa tcttgccgtc cttcacttct acgttcatcg
     1981 ctccccaacg gcccgcggtg agaattttgc cgcccttctc ttctgcccat gcaggtaacg
     2041 gtgcagccga tgtcaccacc agcgcccctg ccgcaatacc gctatgttta ataaattcac
     2101 gtcttgttaa tgtcataact tcctccctga tcaacgagga tcactgtttc tcggtaatat
     2161 ctttggcgtt gtactggaag taccgcgtca aaatgtccag ttcgttttcg ctcatgctgg
     2221 ttcgcgcacc cattcctttg gcaatggacg gccatgtatt gacggtgtaa tggtcggcgg
     2281 caataggggc atggcagcca gcgcaatagg tatcgtcaag tttttcagcg tattgccata
     2341 gcggtttacg gtccgctaat gcgggatcgg taagcgcacc ctgtaaagac gcctgacgcc
     2401 attgattgcc gtattcgtca gcctgccatt ccccgtttac cgctagcgcc ttgatacctt
     2461 cttcacttaa tgtggctagc gccagccgtt gacctgccgc ccggtagagc gtgttttcac
     2521 tgccctgcat ttgataaccc tgcaaccgaa caattggctg tttgccactg gcatcaacga
     2581 cggtgagatc ggtaccagga gtcacggtag ccagttcgcc tatgcgagaa gttttgaaag
     2641 gataaatatg tgcgccatta gtcactgaag tagcggcctg actttccagc tcatgcgccg
     2701 cgttgtcatc catttttatt tctggcggga aatgggcaat gcctttatgg caatcgatac
     2761 aagtttcgcc gtccttttgc gctttattat gcattttctg cgcagattca ctttgcgaag
     2821 caatatccat ggcatcaaaa gaatggcaac tacggcaggt tgcagagtca gttgctttta
     2881 attctttcca tactgtttcg gccatttcct ggcgatgagc ttcgaactta tcgtcactgt
     2941 ctattttgcc gctaacaaat tcatgataaa tatctttaga tgcttttaat ttagcaaata
     3001 aataatccat ccctgacttt ggaatatggc aatcggcaca ttccgcacgt atacctttct
     3061 ggttcgaaaa gtgggcagtt ccctgatatt cctcaaaagg tttactcatc gagtggcagg
     3121 aaagacaaaa tgcggtatcc gacgttttat gtaaggcttt ttgcgccagc aataacccgc
     3181 cgccaccaac cacaaccgct atcagcaaaa acaataaccc aatgcgtttt ttccctcgca
     3241 tagattcctc taattaaatt ccaggacacc gacaccattg tataaataaa tatacaacga
     3301 attgactact tgcttaattc ggcacaacac tgcctgaaat aatcgataaa gaatatgatt
     3361 tattacaatg taatcattaa ttgctaagga ataacccagt tgttatttag aatatatatt
     3421 tgtcagtttt ggcaatttaa atcacataat gaatgtgaat atataaaata tttttagcac
     3481 aacgctatgc gcactgtcac ccgctcaaca gatcacggca atcgttcgtt ttttatactg
     3541 ctcagggata aaaaaaggcc cctgttgaaa ttgcaggggc ctggtacgag caagcatcat
     3601 attgggcgac atgatgcaac ggtaaaaatc atttggcctg atggcgttca atgattcctt
     3661 tcatttcagc aaccgccgcc ccgtctacga cataacgcga atactcgtcc gcgtgtgcat
     3721 ctgatgacat ggacaatcct tgattacgat aacgcatcgg tgaggcttgc cacgctcccg
     3781 cggagctatg gacttccagc actccggcat cgaggaagtg gtgcaggttt tccgcacgga
     3841 ttcctgctcc ggccataatg attggagcat caccatgggc aataagttcc ataatttttg
     3901 ataaaccttg cagtgcgtct gatttttgcc ctgatgtcag tacccgggta atgcccaatt
     3961 ccgcaagatt attgagtgta tttaaagggt tagcgcacat atcgaaggcg cgatgaaaag
     4021 tcactgccag cggaccggca gcagccatta ttttttccat tcgtggcata tcgacattcc
     4081 cgtcaacatc gagaacgccc gtcaccagtc caggaaaacc taattcgcgg actgtgcgca
     4141 catcctcaag aatggcggca aattcaccgt cgctgtagca aaaatcacca ccccgtgggc
     4201 ggataatcgg atgcacaggg atcgtcaccc gctggcgcac ggatttcagt acccccaacg
     4261 acggcgttaa gcccccctct tttggagctg cgcataattc aactctgtca gcgccgtttt
     4321 gctgcgccgt tagcgcgcat tccatgctgt aacagcaaat ttccagtaat gccatttttg
     4381 ctccttagtt acgccgactg ctcgctggca acgatctctt caatggacca cggatgaaac
     4441 ttaatggttg ttttaccatc ggtgaccgcc agcgtcggat tcggtaatcg ttcatgctca
     4501 ccttttggcg aactggtttt aactgagatc cccggcaggc tgagtccttc atcagaaagc
     4561 aatgccagcg cacgggcatt cagcgtttct ggatcacccg gcagaacgat ttcaatatgt
     4621 tcccaacctt cgtgcgggta acgtttttcc ccaggccacg gtagctccac aatggaaaac
     4681 tgccagtgag caacctgtac cggttcatgc aatttaaaca gacaaatcgg tctgccattg
     4741 atcatatttt ctgacaaaag ctcgccgcac tgttcaaacc cgcgacgcca gcgctcagcg
     4801 gtggcgtttt gatggcaacg caaggaaatg tgatcggcag tcagcggagt gatatccaac
     4861 cccagacggc gggaaagttc atctaatgcg tgggtaaatc gcggtaaatc cgatgcaata
     4921 tcctgcagct cgtcgataga ttgccagttc gccataatca ctcttcgtct tccagtaaaa
     4981 gcgttaattt accctgttgc cctgcgccaa ccaaccgctg atttcacgcc gcttctgatg
     5041 caatagtgaa aacgacaata cgccacgcac acgttgctga cgaaaacagc catttgcagt
     5101 atactcccgc cctaatttct ttaactggtg cgggcaaatt ttgctcgctt catcaatgta
     5161 aggtattccg gtgaatattc aggctcttct ctcagaaaaa gtccgtcagg ccatgattgc
     5221 ggcaggcgcg cctgcggatt gcgaaccgca ggttcgtcag tcagcaaaag ttcagttcgg
     5281 cgactatcag gctaacggca tgatggcagt tgctaaaaaa ctgggtatgg caccgcgaca
     5341 attagcagag caggtgctga ctcatctgga tcttaacggt atcgccagca aagttgagat
     5401 cgcgggtcct ggctttatca acattttcct tgatccggca ttcctggctg atcatgttca
     5461 gcaggcgctg gcgtccga
//
LOCUS       DAMZFT010000232         5362 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.232, whole genome shotgun sequence.
ACCESSION   DAMZFT010000232 DAMZFT010000000
VERSION     DAMZFT010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5362)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5362)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5362
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.232"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(<1..1264)
                     /locus_tag="PN977_004963"
     CDS             complement(<1..1264)
                     /locus_tag="PN977_004963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313286.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6044880.1"
                     /translation="MLVKTPVNPLLQWLNMFFSRRSLSGADGRALYAYRCTDTEYESL
                     AELLRTYAPRSYPRTIFISYSDVLFSIYAAEFIRRTHTVGHPKWDTILDSIDWKVPYV
                     HRQKLVNDGIRYWKRKIRNLGQASGYLHTLACEGGLPIRMIENESGYLITYFRRIYQA
                     LRGQASQYPAAKIAQELGDTIPVTMQNELVYEIAGEFCETLCRLLSEHPPQSSDPVSA
                     LRKLSPDWHLQLPLVLPEANAAEIVRRLLSQSSEIRSASSLQVERIWVDVDDSWYCDA
                     RFRFPATMRTEQLTSLFECNIQPEQTRLIISGKWRNGGARLAMLSRYEQQDWRVELLP
                     IAMQKLSGADAMAEISLSLHEGPILLGHTIPKGGYELTEELPWVFEAMNESESQLKLV
                     GMGSVSSRLNALFISLPKNSHLDISGEGE"
     gene            1476..3128
                     /locus_tag="PN977_004964"
     CDS             1476..3128
                     /locus_tag="PN977_004964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HDI6044881.1"
                     /translation="MLAQLFEQLFQSIDSTLITNIFIWAVIFVFLSAWWCDKKNIHSK
                     FREYAPTLMGALGILGTFIGIIIGLLNFNTESIDTSIPVLLGGLKTAFITSIVGMFFA
                     ILFNGMDAFFFANKRSALAENNPESVTPEHIYHELKEQNQTLTKLVSGINGDSEGSLI
                     AQIKLLRTEISDSSQAQLANHTHFSNKLWEQLEQFADLMAKGATEQIIDALRQVIIDF
                     NENLTEQFGENFKALDASVKKLVEWQENYKTQVELMSEQYQQSVESLVETKTAVAGIW
                     EECKEIPLAMSELREVLQVNQHQISELSRHLETFVAIRDKATTVLPEIQNKMAEVGEL
                     LKSGAANVSASLEQTSQQILLNADSMRVALDEGTEGFRQSVTQTQQAFASMAHDVSNS
                     SETLTSTLGETITEMKQSGEEFLKSLESHSKELHRNMEQNTTNVIDMFSKTGEKINHQ
                     LSSNADNMFDSIQTSFDKASAGLTSQVRESIEKFALSINEQLHAFEQATEREMNREMQ
                     SLGNALLSISKGFVGNYEKLIKDYQIIMGQLQALISANKHRG"
     gene            3135..3842
                     /locus_tag="PN977_004965"
     CDS             3135..3842
                     /locus_tag="PN977_004965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HDI6044882.1"
                     /translation="MDKIIGKQLPKKDQDNEHWVSMSDLMAGLMMVFMFISIAYMHYV
                     RIEKEKIKEVAVAYENAQLQLYNALDIEFAKDLQDWDAEIDKQTLEVRFKSPDVLFGL
                     GSTELKPKFKLILDDFFPRYLKVLDNYQEHITEVRIEGHTSTDWTGTTNPDIAYFNNM
                     ALSQGRTRAVLQYVYDIKNIATHQQWVKSKFAAVGYSSAHPILDKTGKEDPNRSRRVT
                     FKVVTNAELQIRKIIQE"
     gene            3846..4949
                     /locus_tag="PN977_004966"
     CDS             3846..4949
                     /locus_tag="PN977_004966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HDI6044883.1"
                     /translation="MKLSIDISELIQLGKKMLPEGVDFFLDESPIDFDPIDIELSTGK
                     EVSIEDLDPGSGLISYHGRQVLLYIRDHSGRYDAAIIDGEKGKRFHIAWCRTLDEMRH
                     KNRFERYHATNRIDGLFEIDDGSGRSQDVDLRVCMNCLERLNYKGSIDKQRKREIFKS
                     FSLNEFFSDYSTCFRHMPKGIYDKTNSGYVENWKDISKSIREKAKYTCNDCGVNLSTA
                     KNLCHVHHKNGIKYDNHHENLLVLCKDCHRKQPLHEGIFVTQAEMAIIQRLRSQQGLL
                     KAESWNEIYDLTDPSVHGDINMMQHKGFQPPVPGLDLQNSEHEIIATVEAAWPGLKIA
                     VNLTPAEVEGWRIYTVGELVKEIQTGAFTSATL"
     gene            5159..>5362
                     /locus_tag="PN977_004967"
     CDS             5159..>5362
                     /locus_tag="PN977_004967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418730.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose oxidase"
                     /protein_id="HDI6044884.1"
                     /translation="MNKAITVLAIMMASFSVNAYVLPETPVPFKSGTGEIDNNTVYIG
                     LGSAGTAWYKLEKQTKDKKMDSVS"
BASE COUNT         1723 a         1030 c         1084 g         1525 t
ORIGIN      
        1 actcaccttc tccactaata tctaaatggc tatttttcgg tagcgaaata aacagagcat
       61 ttagcctgga actcacagac cccataccga caagttttag ctgcgattca ctctcattca
      121 tcgcttcaaa aacccagggg agttcttcag taagttcata accgcctttt ggaattgtgt
      181 ggcctaacag aattggacct tcatgaagcg acaacgagat ttcggccatt gcatctgcac
      241 cagagagctt ttgcatcgca ataggtaata actcgacccg ccaatcttgc tgctcataac
      301 ggcttagcat tgccaacctt gccccgccat ttctccattt tcctgaaatg atgagtcggg
      361 tctgctccgg ctgaatattg cattcaaaca aagaggttaa ctgttctgta cgcattgtgg
      421 ccgggaatcg gaaccgggca tcacaatacc aactgtcatc aacatcgacc cagattcttt
      481 caacctgaag actacttgca ctgcgtattt cagaagattg agaaagtaat cgccttacaa
      541 tctctgcagc attcgcttcg gggagaacga gtggaagttg aaggtgccag tctggagaga
      601 gcttacgtaa tgcagaaacg ggatcactgc tttgaggtgg atgctcacta agtaatcggc
      661 agagagtctc gcagaattca ccggctattt catagaccag ttcgttttgc attgtgacag
      721 gaatcgtatc gcctaattct tgtgcaattt tagcggcagg atattgagat gcttgcccac
      781 gaagcgcctg atatatcctt ctgaaatagg taatcaaata accgctctca ttttcaatca
      841 tgcggatagg taaaccacct tcacaagcca gtgtatgcag ataacccgaa gcttgcccca
      901 ggtttcttat ctttcttttc cagtagcgaa tgccatcatt aaccagcttc tgtcgatgaa
      961 catacggaac tttccagtca atagaatcta aaattgtgtc ccatttaggg tgtccgaccg
     1021 tatgagtccg gcggataaac tctgcggcat atatgctaaa tagaacatcg ctataggaaa
     1081 tgaatatcgt tcttggataa ctacgggggg catatgtacg cagtagttct gccaaacttt
     1141 cgtactctgt atcagtacaa cggtaagcat ataacgcccg tccatcagct ccggataatg
     1201 aacggcgact aaaaaacatg ttaagccatt gcaaaagtgg gttaactgga gttttcacaa
     1261 gcaacctgcc ttcctttgat acaattcgta acaggttact atcatcataa aaaagctcaa
     1321 cccgatgaac tcgctaaaaa tgagacaaat catttatatc tcgaaaaaac ttgttacaat
     1381 catgagcgct acaccgaact taaccatata aattatgtgt gttttgttta ttttttaaac
     1441 aattacaact atccattatt tacacaggta tcaaaatgtt agcgcagctt tttgagcagt
     1501 tgtttcaatc gatagactct acactgatca ccaatatttt catctgggct gttatattcg
     1561 tatttttatc agcgtggtgg tgtgacaaaa aaaatataca tagtaagttt agagaatatg
     1621 ctccaacctt aatgggggca ttaggtattc tgggtacttt cattggtatt attattgggt
     1681 tactcaattt taatactgaa agtattgata ccagcatccc cgtattatta ggcggcctaa
     1741 aaacagcatt cattacaagc attgtaggta tgttttttgc cattttattt aatggaatgg
     1801 atgctttctt ttttgccaat aaacgaagtg cgttagcaga aaataaccct gaatctgtta
     1861 cacctgaaca tatctatcat gaattaaaag agcagaacca gactctgact aaattagtct
     1921 cgggtattaa cggtgatagt gaaggttctc ttattgctca aataaaatta ctacgtactg
     1981 agattagcga ttcctcgcag gcacaattag ctaatcacac tcatttcagt aataagcttt
     2041 gggaacaact tgaacaattt gcagatctaa tggcaaaagg tgctacagaa caaattattg
     2101 atgctttacg acaagtcatt attgatttta atgaaaattt aactgaacag tttggtgaaa
     2161 actttaaagc acttgatgcc tctgtaaaaa aacttgttga gtggcaggaa aattataaaa
     2221 cgcaagttga gctgatgtca gaacaatatc aacaaagtgt cgagtctctg gttgaaacaa
     2281 aaactgcggt tgccgggatt tgggaagaat gtaaagaaat tcctctggct atgtctgaac
     2341 tgcgtgaagt gcttcaggtg aaccaacatc aaatcagcga actctcccgc catttagaaa
     2401 cctttgtcgc catccgcgat aaagctacaa ccgtattacc tgaaatacag aacaaaatgg
     2461 ctgaagtggg tgaactgctg aaatccggag ctgcaaatgt tagtgcatct cttgagcaaa
     2521 ccagccagca aatacttctt aatgcagatt caatgcgcgt ggccctggat gaaggtaccg
     2581 aaggattcag acaatcggtt acccaaacac aacaagcatt tgcctcgatg gcacatgatg
     2641 tcagcaattc ctccgaaact ctaaccagca cgttaggtga aacaattact gaaatgaaac
     2701 aaagtggtga agaattcctg aagtcactag agtcgcactc gaaagaattg catagaaata
     2761 tggaacaaaa tacgactaat gtaattgata tgttcagtaa gactggtgaa aagattaacc
     2821 atcaactatc cagtaatgcc gataatatgt ttgattcaat ccagacatca tttgataagg
     2881 caagtgcagg gctgacttct caagtcagag aatcaattga aaaatttgct ctatccatca
     2941 acgagcagtt acatgctttt gagcaagcaa ctgaacgtga gatgaaccgt gaaatgcaat
     3001 cattaggtaa tgctctgctt tcaatcagca aaggttttgt cggtaactat gaaaaactta
     3061 ttaaagatta ccaaataatt atggggcagt tacaagcatt aatttctgct aataaacatc
     3121 gagggtaatc gatcatggat aagattatag ggaaacaatt acctaaaaaa gatcaagata
     3181 atgaacactg ggtatccatg tcagacctaa tggcagggct gatgatggtt tttatgttca
     3241 tatctattgc ttatatgcac tacgtacgta ttgaaaaaga aaaaattaaa gaagttgccg
     3301 tagcctacga gaatgctcag ttacagcttt ataatgctct ggatattgag tttgcaaagg
     3361 atttgcaaga ctgggatgca gagatcgata agcagactct ggaggttcga tttaaatcac
     3421 cggatgtttt atttggctta ggaagcacag agcttaaacc aaagtttaaa ctcattcttg
     3481 acgacttctt tcctcgctac ctaaaagttc ttgataatta tcaagaacat attactgaag
     3541 tccgcattga aggtcacaca agtactgact ggactggaac aacgaatcct gatattgctt
     3601 attttaataa tatggcacta tcacaaggtc gtacacgtgc agtattacaa tacgtttatg
     3661 atataaaaaa tatcgcgaca caccaacaat gggttaaaag taaatttgcc gcagtaggtt
     3721 attcatctgc acatcccatt cttgataaaa ccggcaagga ggatcctaat cgctctcgtc
     3781 gtgtcacctt caaagttgta acaaatgccg agttgcagat tagaaagatt attcaggagt
     3841 aagagatgaa attatctatc gacatttcag aacttattca attagggaag aaaatgttac
     3901 cagaaggagt cgattttttt ctggatgaat cccctattga ctttgatcct atagatattg
     3961 agttatccac gggtaaagaa gttagtatcg aagatcttga ccctggtagc gggctaatct
     4021 cttatcatgg ccgccaggtt cttttatata ttagggacca ttcagggcgt tatgatgcgg
     4081 ctatcatcga tggcgaaaaa ggaaaacgtt ttcatattgc ctggtgcaga actcttgatg
     4141 aaatgcgcca taaaaatcga tttgaaaggt atcatgcaac taaccgcata gatggtttat
     4201 tcgaaattga tgatggttca ggccggagcc aggatgttga tttacgggta tgtatgaatt
     4261 gtctggaacg acttaattac aaaggaagta ttgataaaca aaggaaaaga gagattttta
     4321 aatcattctc attaaatgag tttttttcag attatagtac ctgttttcgt catatgccta
     4381 agggtatcta tgacaaaaca aatagtgggt atgtcgaaaa ctggaaagat atatcaaaat
     4441 caatacgaga aaaggccaag tatacttgta atgattgtgg tgtgaattta tcaaccgcca
     4501 aaaacttgtg ccatgtccat cataaaaatg gcatcaaata tgataatcac catgaaaacc
     4561 ttcttgttct gtgtaaggat tgccatcgta aacagcccct ccatgaaggt atattcgtta
     4621 cccaagctga gatggctatc attcaacgtt tacgttccca acaagggtta ttaaaagccg
     4681 aatcctggaa tgaaatatat gacctgactg atccatcagt acatggtgat attaatatga
     4741 tgcaacataa aggctttcaa cctcctgttc ctgggttaga tcttcaaaac tcagaacatg
     4801 aaattattgc aaccgtagaa gcagcatggc caggccttaa aattgcagtt aaccttactc
     4861 ccgccgaagt cgaaggatgg agaatatata ccgtgggtga gctggttaaa gaaatacaaa
     4921 caggagcctt tacgtcagca acgttgtaaa ttccgaaact ccgtgaaagt taagactttc
     4981 acggaagata aataaaattt tcctgatttg tgactcaaat tacaaaagtc gtttatggca
     5041 taacttgtct gtttttttgg tgtaataggt ataaaggcat atgctatggt tagccttata
     5101 tttaaaactt tcctcatacg ggtgaaggtt aaagcttggt agataggtta cagtcacaat
     5161 gaataaagca ataacggtgc ttgctataat gatggcttca ttttccgtaa atgcgtatgt
     5221 attaccggaa acgcctgtgc catttaaaag tggtaccgga gaaattgata acaacactgt
     5281 ctacattggt ttaggtagcg caggtacggc atggtacaag ctggagaaac agaccaaaga
     5341 taaaaaaatg gacagcgtta gc
//
LOCUS       DAMZFT010000233         5322 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.233, whole genome shotgun sequence.
ACCESSION   DAMZFT010000233 DAMZFT010000000
VERSION     DAMZFT010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5322)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5322)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5322
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.233"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            159..1037
                     /gene="glxR"
                     /locus_tag="PN977_004968"
     CDS             159..1037
                     /gene="glxR"
                     /locus_tag="PN977_004968"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HDI6044885.1"
                     /translation="MKLGFIGLGIMGTPMAINLARAGHQLHVTTIGPVADELLSLGAV
                     SVETARQVTEASDIIFIMVPDTPQVEDVLFGENGCTKASLKGKTIVDMSSISPIETKR
                     FARQVNELGGDYLDAPVSGGEIGAREGTLSIMVGGDEAVFERVKPLFELLGKNITLVG
                     GNGDGQTCKVANQIIVALNIEAVSEALLFASKAGADPVRVRQALMGGFASSRILEVHG
                     ERMIKRTFNPGFKIALHQKDLNLALQSAKALALNLPNTATCQELFNTCAANGGSQLDH
                     SALVQALELMANHKLA"
     gene            1207..2661
                     /locus_tag="PN977_004969"
     CDS             1207..2661
                     /locus_tag="PN977_004969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative allantoin permease"
                     /protein_id="HDI6044886.1"
                     /translation="MEHQRKLFQQRGYSEDLLPKTQSQRTWKTFNYFTLWMGSVHNVP
                     NYVMVGGFFILGLSTFSIMLAIILSAFFIAAVMVLNGAAGSKYGVPFAMILRASYGVR
                     GALFPGLLRGGIAAIMWFGLQCYAGSLACLILIGKIWPGFLTLGGDFTLLGLSLPGLI
                     TFLLFWLVNVGIGFGDGKVLNKFTAILNPCIYIVFGGMAIWAISLVGIGPIFDYIPGG
                     IQKAENGGFLFLVVINAVVAVWAAPAVSASDFTQNAHSFREQALGQTLGLVVAYILFA
                     VAGVCIIAGASIHYGADTWNVLDIVQRWDSLFASFFAVLVILMTTISTNATGNIIPAG
                     YQIAAIAPTKLTYKNGVLIASIISLLICPWKLMENQDSIYLFLDIIGGMLGPVIGVMM
                     AHYFVVMRGQINLDELYTAAGDFKYYDNGFNLTAFSVTLVAVILSLGGKFIPFMEPLS
                     RVSWFVGVIVAFAAYALLKKRTAAEKTGEQKVTG"
     gene            2722..4083
                     /gene="allB"
                     /locus_tag="PN977_004970"
     CDS             2722..4083
                     /gene="allB"
                     /locus_tag="PN977_004970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="allantoinase AllB"
                     /protein_id="HDI6044887.1"
                     /translation="MSFDLIIKNGTVILENEARVVDVAVKGGKIAAIGQDLGDAKEVM
                     DASGLVVSPGMVDAHTHISEPGRSHWEGYETGTRAAAKGGITTMIEMPLNQLPATVDR
                     ASIELKFDAAKGKLTIDAAQLGGLVSYNIDRLHELDEVGVVGFKCFVATCGDRGIDND
                     FRDVNDWQFFKGAQKLGELGQPVLVHCENALICDELGEEAKREGRVTAHDYVASRPVF
                     TEVEAIRRVLYLAKVAGCRLHVCHISSPEGVEEVTRARQEGQDVTCESCPHYFVLDTD
                     QFEEIGTLAKCSPPIRDLENQKGMWEKLFNGEIDCLVSDHSPCPPEMKAGNIMKAWGG
                     IAGLQSCMDVMFDEAVQKRGMSLPMFGKLMATNAADIFGLQQKGRIAPGKDADFVFIQ
                     PNSSYVLTNDDLEYRHKVSPYVGRTIGARITKTILRGDVIYDIEQGFPVAPKGQFILK
                     HQQ"
     gene            4139..5212
                     /locus_tag="PN977_004971"
                     /pseudo
     CDS             4139..5212
                     /locus_tag="PN977_004971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308602.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="uracil/xanthine transporter"
     gene            5269..>5322
                     /locus_tag="PN977_004972"
     CDS             5269..>5322
                     /locus_tag="PN977_004972"
                     /inference="COORDINATES: protein motif:HMM:NF015750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6044888.1"
                     /translation="MASVSISCPSCSATDGVV"
BASE COUNT         1191 a         1215 c         1427 g         1489 t
ORIGIN      
        1 aatataaacc ccaaaccacc acggaagctg gtttacgctg gatggatccg taccgttaaa
       61 acgtaacgct attcagacaa tgctttttta ggtcgctaag tcggcagggg atcgtgttgt
      121 ctgaattcag gaaaagcgaa atttaaaaga ggttaattat gaaactggga tttattggct
      181 taggcattat gggtactccg atggccatta atctggcgcg tgccggtcat caactgcatg
      241 tcacaaccat tggtccggtt gctgatgaat tactgtcact gggtgccgtc agtgttgaaa
      301 ctgcgcgcca ggtaacggaa gcatcggaca tcatttttat tatggtgccg gatacgccac
      361 aggttgaaga cgttctgttt ggtgaaaatg gttgtaccaa agcctcgctg aagggcaaaa
      421 ccattgttga tatgagctcc atttccccga tcgaaactaa acgtttcgct cgtcaggtga
      481 atgaactggg cggcgattat ctcgatgcgc ccgtctccgg cggtgaaatc ggcgcgcgtg
      541 aagggacgtt gtcgattatg gttggcggtg acgaagcggt atttgaacgt gttaaaccgc
      601 tgtttgaact gctcggtaaa aatatcaccc tcgtgggcgg taacggcgac ggtcaaacct
      661 gcaaagtggc aaatcagatt atcgtggcgc tcaatattga agcggtttct gaagccctgc
      721 tatttgcttc aaaagccgga gcggatccgg tacgtgtgcg ccaggcgctg atgggcggct
      781 ttgcttcctc acgtattctg gaagttcacg gtgagcgcat gattaaacgc acctttaatc
      841 caggttttaa aattgcgctg caccagaaag atctcaacct ggcactgcaa agtgcgaaag
      901 cactggcgct gaatctgcca aacaccgcga cctgccagga attgtttaat acctgtgcgg
      961 caaacggtgg tagccagttg gatcactctg cgttagtgca ggcgctggaa ttaatggcta
     1021 accataaact ggcctgatac ccgcaataaa aatggccgat atcagaaaat gaatcggcca
     1081 gcaatattaa aaaaagaaag cagccaaaga tgttgcttca gtattaaaaa taatattttt
     1141 attttatttg ttcctcatag ctagattaaa aagacgttat tcgatacgtg aaattaagag
     1201 ggatttatgg aacatcagag aaaactattc cagcaacgcg gctatagcga agatctattg
     1261 ccgaaaacgc aaagccagcg gacctggaaa acatttaact attttacctt atggatgggt
     1321 tcggttcata acgttcccaa ttatgtgatg gtcggcggct tttttattct cggcttgtct
     1381 acctttagta ttatgctggc aattatcctc agcgcctttt tcattgccgc ggtaatggta
     1441 ttaaacggtg ctgcgggcag taaatacggt gtgccttttg ccatgatcct gcgtgcttct
     1501 tacggcgtac gtggtgcact gtttcccgga ttattaagag gcgggattgc tgccattatg
     1561 tggtttggcc tgcaatgtta cgctggatcg ctggcctgct tgatcctgat tggcaaaatc
     1621 tggccgggat ttttaactct cggtggtgat tttaccctgt tagggctttc tctaccgggt
     1681 ttaatcactt tcttactctt ctggctggtg aacgtcggaa tcggtttcgg tgatggcaaa
     1741 gttttaaata aattcactgc cattcttaac ccgtgcatct atatcgtttt cggcggtatg
     1801 gcgatttggg cgatttcgct ggtcgggatc ggtccaatct ttgactatat tccgggcggt
     1861 attcagaaag cagaaaacgg tggcttcctg ttcctggtgg tgattaacgc ggtagttgcg
     1921 gtctgggcgg caccggcggt gagcgcatcc gactttacgc aaaacgccca ctcgtttcgt
     1981 gagcaggcgc tggggcaaac gctgggttta gttgtggcct atattctgtt tgcggtcgcc
     2041 ggggtatgta ttattgccgg agccagtatt cactacggcg cggacacctg gaacgtgctg
     2101 gatattgttc agcgttggga cagcctgttt gcctcgttct ttgcggtact ggttattctg
     2161 atgacgacta tctccactaa cgccaccggt aatattattc cggctggtta tcagatagcc
     2221 gctattgcac cgacgaaact gacctataaa aacggcgtac tgattgccag tattatcagc
     2281 ttgctgatct gcccgtggaa attaatggaa aatcaggaca gcatttatct gttcctcgat
     2341 attatcggcg gaatgctggg tccggtaatt ggtgtcatga tggcgcatta ttttgtggtg
     2401 atgcgcggac aaattaatct tgatgaactg tataccgcag caggggattt caaatattac
     2461 gataacggct ttaaccttac cgcgttctca gtaactttgg tcgccgttat tttatctctc
     2521 ggtggtaaat ttattccgtt catggagcct ttatcacgcg tttcatggtt tgtcggcgtt
     2581 attgtcgcct ttgcggccta cgccttatta aagaagcgta ctgcagcaga aaaaacagga
     2641 gaacaaaaag tcacaggtta attaatcccg atattgaaca ttgagttaaa aaaccaatct
     2701 gtattttaca aggagtttgt tatgtctttt gatttaatca ttaaaaacgg caccgttatt
     2761 ttagaaaacg aagctcgcgt agtggatgtc gccgttaaag gcggaaaaat tgctgctatc
     2821 ggtcaggatc tgggcgatgc aaaagaagtt atggatgcgt ctggtctggt ggtttcgcca
     2881 ggcatggttg atgcgcacac ccatatttct gaaccgggtc gcagccactg ggaaggttat
     2941 gaaaccggta ctcgcgcagc agcaaaaggt ggtatcacca ccatgatcga aatgccgctc
     3001 aaccagctgc ctgcaacggt tgaccgcgca tcgattgaac tgaagtttga tgccgccaaa
     3061 ggcaagctga ctatcgatgc ggcgcaactc ggtggcctgg tgtcttacaa catcgaccgt
     3121 ctgcatgagc tggatgaagt gggcgttgtc ggcttcaaat gcttcgttgc gacctgtggc
     3181 gatcgcggta tcgacaacga cttccgtgac gtcaatgact ggcagttctt caaaggtgcg
     3241 cagaagctgg gcgaactggg acagccggtg ctggtgcact gcgaaaacgc gctgatctgt
     3301 gacgaacttg gcgaagaagc gaaacgtgaa ggtcgcgtaa ccgcacatga ctatgtggct
     3361 tcgcgtccgg tatttaccga agtggaagcg attcgccgcg tgctgtacct ggcgaaagtt
     3421 gccggttgcc gtctgcacgt ttgccatatc agcagcccgg aaggtgttga agaagtgact
     3481 cgtgcacgtc aggaaggcca ggatgttacc tgtgaatcct gcccgcatta ctttgtgctg
     3541 gataccgatc agttcgaaga aattggcacc ctggcgaagt gttcaccgcc gatccgcgat
     3601 ctggaaaacc agaaaggcat gtgggaaaaa ctgtttaacg gtgaaataga ctgcctggtt
     3661 tccgaccact ctccatgccc gccggaaatg aaagccggta acatcatgaa agcgtggggc
     3721 ggtatcgctg gtctgcaaag ctgcatggac gtgatgttcg atgaagcggt acagaaacgc
     3781 ggaatgtctc tgccaatgtt cggcaaatta atggcgacta acgcagcaga tattttcggt
     3841 ctgcagcaaa aaggccgtat cgccccagga aaagatgccg acttcgtctt cattcagccg
     3901 aatagcagct atgttcttac caatgacgat ctggaatatc gccacaaagt cagcccgtat
     3961 gttggccgta ctattggcgc gcgtatcacg aaaaccatct tacgtggtga tgtgatttac
     4021 gacatcgaac agggcttccc tgttgcgccg aaaggtcaat ttatccttaa acatcagcag
     4081 taatctggcc ctgcaatgcc cgtccttgtg gcgggcattc tccggttaag gtgtgtttat
     4141 gttcaatttt gcagtgggcc gcgaaagcct gttatcagga tttcagtggt ttttctttat
     4201 tttttgcaac acggttgtgg ttcctcctac gctactttct gcttttcagt tgccgcaaag
     4261 tagcctgctt acgctcacgc aatatgcttt tcttgctacc gcactggcct gcttcgctca
     4321 ggcgttctgc ggtcatcgtc gcgctattat ggaagggccg ggtggcctgt ggtggggaac
     4381 catcctgact atcacccttg gcgaagcatc gcgcgggaca ccgatcaacg atatcgctac
     4441 cagcctggcg gtgggaattg cgctctctgg cgtgctgacg atgttgattg gttttagcgg
     4501 attaggtcat cgtctggcgc ggttatttac gccgtcggtg atggtcttgt ttatgttgat
     4561 gctgggcgcg cagctgacca ctatcttttt caaaggtatg ctcgggctgc cgtttggcat
     4621 agccgacccg aattttaaaa ttcagttacc gccgttcgcg ctctcggtgg cggtgatgtg
     4681 cctggtactg gcgatgatta tcttcctgcc gcaacgtttt gcccgttatg gcctgctggt
     4741 cggtacgata accggctggt tgttgtggta cttttgcttt ccttcttcgc attcgctctc
     4801 cggtgagttg cactggcagt ggttcccgct cggcagtggc ggtgctttgt cgccggggat
     4861 tattctgacg gcggtgatta caggtctggt aaatatcagc aatacctacg gtgcgattcg
     4921 tggcacggat gttttttatc cgcaacaggg cgcggggaat acgcgttatc gtcgcagctt
     4981 tgtggcgacc ggatttatga cgctgataac cgtgccgctg gcggtaattc cattttcacc
     5041 gtttgtttca tccattggtt tattaaccca gactggcgat tacacgcgac gatcgtttat
     5101 ttatggcagc gttatttgcc tgctggtggc actggttccg gcactcacac gactgttttg
     5161 cagtatccct ttaccggtga gtagcgcggt catgttggtt tcttatctgc cttggtgatg
     5221 ctgccaactt actgatttag tgtatgatgg tgtttttgag gtgctccagt ggcttctgtt
     5281 tctatcagct gtccctcctg ttcagctact gacggggtgg tg
//
LOCUS       DAMZFT010000234         5295 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.234, whole genome shotgun sequence.
ACCESSION   DAMZFT010000234 DAMZFT010000000
VERSION     DAMZFT010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5295)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5295)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5295
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.234"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..192
                     /locus_tag="PN977_004973"
     CDS             <1..192
                     /locus_tag="PN977_004973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001434943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HDI6044889.1"
                     /translation="RMPNTKTAVLVAVEFDRKGDWRMKWATYIPGHPDANDGWIIPGA
                     SWKPSHWMPLPEPPQEVNQ"
     gene            135..272
                     /locus_tag="PN977_004974"
     CDS             135..272
                     /locus_tag="PN977_004974"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6044890.1"
                     /translation="METVTLDAATRTAAGGESMSWPEAFTTVGIVMAAALGLYSICRW
                     W"
     gene            275..508
                     /locus_tag="PN977_004975"
     CDS             275..508
                     /locus_tag="PN977_004975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6044891.1"
                     /translation="MGKITFVVEFEDGKEPPVSANLDVAGGRLVSVLFGDYRDDFFQP
                     EEVDVVREALNELSVDNDDAHAEIIQKMELLTH"
     gene            687..980
                     /locus_tag="PN977_004976"
     CDS             687..980
                     /locus_tag="PN977_004976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000269003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6044892.1"
                     /translation="MWRGNSHGKSQMILTEYKLDHKTNKSRSVYLLRHNSRVRNTVLE
                     QNLTVEMDNYGGFKPTISLDDFPRGLSEREAMLKLAEWLQRLSIAIEDNWSEP"
     gene            987..1361
                     /locus_tag="PN977_004977"
     CDS             987..1361
                     /locus_tag="PN977_004977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000988657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6044893.1"
                     /translation="MMTLKHFLDRPLWAAAAGYDFNYMDCMSYTANAYDYSFSLLLNS
                     LRILPQTEVGEIHLWLLGFIAAGVGIAVWPFIFWLVAVVVWFKCKTYRRKYFLGDGMT
                     DIAKMNIEKWTKECEKKWRKKK"
     gene            1343..1993
                     /locus_tag="PN977_004978"
     CDS             1343..1993
                     /locus_tag="PN977_004978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000057456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6044894.1"
                     /translation="MAQKEMTRITEERILEIISRIEMYGHGAGYTADEVLEIAQQMLA
                     LRQKEQHESNTCRLNFEQWLEQQRAKIDVDCGCVSTETFMHWLRVAYEAGNYPDIPDS
                     SVPAPGKGVTGERIRIKPHVYRELVNRIHDTAIKCAGTQQLREKISRVLGDVITPDHH
                     KQAEKSGLERCHLEAALNIKPGHTLGIIDALLVHKMARALLPLVADKHEAGHANES"
     gene            2266..2526
                     /locus_tag="PN977_004979"
     CDS             2266..2526
                     /locus_tag="PN977_004979"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6044895.1"
                     /translation="MFRPRPIPPKITNGYQPNTPEIQGSEGLKPPPAPRIPPVARNVS
                     LRRELILALSPVLAARQYWVSNDDLAEKITSLVDKILHKADL"
     gene            2841..3080
                     /locus_tag="PN977_004980"
     CDS             2841..3080
                     /locus_tag="PN977_004980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001423760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit theta"
                     /protein_id="HDI6044896.1"
                     /translation="MSDWNIAAKSQEERDKVNVDLAASGVAYKERLNIPVIAEQVARE
                     QPENLRTYFMERLRHYRQLSLQLPKGNDPAYQKTE"
     gene            complement(3092..3454)
                     /locus_tag="PN977_004981"
     CDS             complement(3092..3454)
                     /locus_tag="PN977_004981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001062543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6044897.1"
                     /translation="MAKIEYHRDRGNYLEIYDHESLNDINDALYEYCEKMSITDAPDA
                     FVELPVYLRDIYAIRTPPVSVIHIGYVRLSIEEDEDRYIVRHYTLDRKELPNEWNMSN
                     FYNGQYGLKPAKNGAGIA"
     gene            complement(3455..4039)
                     /locus_tag="PN977_004982"
     CDS             complement(3455..4039)
                     /locus_tag="PN977_004982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001133673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HDI6044898.1"
                     /translation="MPNRIPLDPVLPKNFDCTPNEKRSKAQLDAWWDHPYGVTQPDGK
                     IVVYCLNGGAWDRPSVLCLADNYDEACELAERQQASWVKTRSEPTFMFSKEPPFILAR
                     MPQRPDHQQEIVAEFSSRDEMNLFSLKQEERDRVEVSPTLDHNRMNLAQLAWYSKELE
                     MSIARLENEKAAIQAQHEVVLNRIREMQNDNRGF"
     gene            4214..4603
                     /locus_tag="PN977_004983"
     CDS             4214..4603
                     /locus_tag="PN977_004983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000506727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein"
                     /protein_id="HDI6044899.1"
                     /translation="MGFPSPAADYVESRISLDQQIIRHPSATYFMRAADSHHREGILQ
                     GALLVVDSSLTPVDGSLLVCAMEGEYRIKRYRKYPRQHLEDLSTGKKEALPVDDDSYT
                     GSNAVFGVITHVINDARSGEFDDCPVI"
     gene            4676..4897
                     /locus_tag="PN977_004984"
     CDS             4676..4897
                     /locus_tag="PN977_004984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001603498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HDI6044900.1"
                     /translation="MQSINFRTARGKLSEVLNNVEAGEEVEITRRGREPAVIVSKATF
                     EAYKKAALDAEFASLFDTLDSTNKELVNR"
     gene            4897..5277
                     /locus_tag="PN977_004985"
     CDS             4897..5277
                     /locus_tag="PN977_004985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001216034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HDI6044901.1"
                     /translation="MRHISPEELIALHDANISRYGGLPGMSDPGRAEAIIGRVQARVA
                     YEEITDLFEVSATYLVATARGHIFNDANKRTALNSALLFLRRNGVQVFDSPELADLTV
                     GAATGEISVSSVADTLRRLYGSAE"
BASE COUNT         1453 a         1092 c         1353 g         1397 t
ORIGIN      
        1 cgaatgccga ataccaaaac agccgttctt gttgccgtgg agtttgacag gaaaggtgac
       61 tggcgaatga aatgggctac ttacatcccg gggcatcctg acgctaatga tgggtggata
      121 attcctggtg cgtcgtggaa accgtcacac tggatgccgc taccagaacc gccgcaggag
      181 gtgaatcaat gagctggcct gaagcattca ccacggtagg aattgtgatg gcggcagcac
      241 tgggtttgta ttcaatttgt cgctggtggt aacgatggga aaaataactt ttgtagtcga
      301 atttgaggat ggtaaagagc cacctgttag cgccaatctt gatgttgctg gtggcaggct
      361 tgtttcggtt ctatttggtg actaccgaga tgatttcttc caaccagaag aagttgatgt
      421 ggtgcgagag gcattaaacg agttaagtgt tgataacgat gatgctcatg cggaaatcat
      481 ccaaaaaatg gaactgctaa ctcactaaat tatcaattat ggtgctatca cctacgacac
      541 cgagagaaaa tttataatgt caaaagtaaa tgttttgatt ttttcagcaa ttgttggctt
      601 tggttttact gccggagtgc agatttatat tgcgtgggaa aaaatcatca actacgcatg
      661 gagttgtttt attaagtgag gtaagtatgt ggagaggtaa tagtcatggc aaaagccaga
      721 tgatacttac cgaatataag ctagaccaca aaaccaataa atcacgttca gtatatttgc
      781 tccggcacaa tagccgcgta agaaataccg tgctggaaca aaatctgaca gttgaaatgg
      841 ataattacgg gggattcaag ccaacaattt cacttgatga ttttcctcgt ggtttaagcg
      901 aaagagaagc aatgctgaaa ttagcagaat ggctacaaag attaagcatt gctattgaag
      961 ataactggtc tgaaccttaa atttatatga tgacactaaa acattttctt gaccgcccat
     1021 tatgggcggc agccgcaggc tatgacttta attatatgga ttgcatgtct tataccgcca
     1081 atgcatacga ctattccttc agtctgctgc ttaattcttt aagaatattg ccgcaaacag
     1141 aagttggaga gattcattta tggctattgg gctttatcgc ggctggagtt ggtattgctg
     1201 tatggccttt tattttctgg ctggtggctg ttgtagtgtg gtttaagtgc aagacgtacc
     1261 ggagaaagta tttcttaggt gatggaatga ctgatattgc caaaatgaac attgaaaaat
     1321 ggactaagga atgtgaaaag aaatggcgca aaaagaaatg accagaatca ctgaagagcg
     1381 tatattagag attatttccc gtatcgaaat gtatggtcac ggtgctggat atacggcaga
     1441 tgaagtattg gaaatagccc agcagatgct ggccttgaga caaaaagagc aacatgaaag
     1501 taatacgtgt agattgaatt ttgagcagtg gctggaacag caacgcgcga aaatcgatgt
     1561 ggactgcggt tgtgtgtcca ctgaaacatt catgcactgg ctgcgggtag cttacgaggc
     1621 tggcaactat ccggatattc cggatagttc ggtgccagcg ccaggaaagg gcgtcaccgg
     1681 tgaacgtatc cgaattaagc cgcatgttta tcgcgaactg gttaaccgca tccacgatac
     1741 agcgatcaag tgtgctggca cccagcaatt acgagaaaaa attagccgtg ttttgggcga
     1801 cgttattacg ccagatcatc ataaacaagc cgagaaaagt ggcctggaaa ggtgtcacct
     1861 tgaggcggca ttaaacatta agccggggca tacgcttggc attattgatg cactattggt
     1921 tcataagatg gccagggctt tattgccgct ggtggctgat aagcatgagg cgggccatgc
     1981 caacgaaagc tgagttacag gcgcgcatag agattcttga aaaagagaat gcgagtctaa
     2041 aaggaatgct ggcgcgggcg gaaagggaat tatcaggcaa attattgcca gaagaactgc
     2101 caccagcaga tataccagat cgagtgtccc ggtggatgaa gtcttttggt ttaccgtggg
     2161 aggctttttg gtgctacgag catcgcagat ggtgtgatga acttgatagc agtttcccct
     2221 actttgcgga agggaacacc tgccctgagt gtaggaggta gcgcgatgtt tcgtcctcgt
     2281 ccaataccgc cgaaaataac taatggctat cagccgaata ctccagaaat acaagggagc
     2341 gaggggctta aaccaccacc agcaccacgt attccgcctg tagcaaggaa cgtatcatta
     2401 cgacgagagt taatattagc cttgtcgccc gttctggctg cccgccagta ttgggtaagt
     2461 aatgatgacc tggcagaaaa aatcacatcg ctggtggata aaattctgca taaagctgat
     2521 ctctgatcac catgctaaaa tcccctcaat tcacagaggg gatttttgtc tatgttgcat
     2581 agagttttat ggaaaagcgt tattgtcggc gcgatactgg ctatatctgg gtgtgcaatg
     2641 gtgcaataca atgacggcga aaaggttagt attcagtcag atggttggta tggcctggat
     2701 agcctgcaaa aaaccgcaga taaagcctgt cagcaatatg ggaagtctaa agcagtatat
     2761 cagcatagcg cgaacgctaa ccctcatctt gcgcccggtt cgggtgttca gaataccatc
     2821 tggaaatgtg agccttaaat atgagcgatt ggaatattgc agctaaaagt caggaagaac
     2881 gggataaggt gaacgttgat ctggctgcca gcggtgtggc gtacaaagag cgattgaaca
     2941 tacctgtcat agctgaacag gtagcccgcg agcaaccaga gaacctgcgc acctatttca
     3001 tggaacgtct acggcactac cggcagttaa gcctccagtt gccaaaaggg aacgatccgg
     3061 catatcagaa aacagaatag ttgttaggct attacgcaat gcccgcgcca ttcttagcgg
     3121 gttttaagcc atattgaccg ttgtagaaat tactcatgtt ccattcattg ggaagctctt
     3181 ttctgtccaa tgtatagtgg cgcacgatat aacgatcttc atcttcttcg atggacaggc
     3241 ggacatagcc aatgtgaatt accgatacgg gcggcgtccg tattgcatag atgtcgcgga
     3301 gatataccgg tagctcgaca aatgcatcag gtgcatctgt gatgctcatt ttttcacagt
     3361 attcatataa cgcatcgttg atatcgttaa gagattcatg atcgtatatt tccaggtaat
     3421 taccgcgatc gcgatggtac tcgattttag ccattcaaaa tcccctgtta tcgttttgca
     3481 tttctctaat ccggttcaga actacttcat gctgggcttg gatagcggct ttttcgtttt
     3541 caagtcgggc aatagacatc tctaattctt tgctgtacca ggcgagttgg gccaggttca
     3601 tccggttgtg gtcgagagtt ggagacactt cgacgcgatc cctttcttcc tgctttaatg
     3661 agaagagatt catctcatcc cttgaggaaa attcagcaac aatttcttgt tgatgatccg
     3721 gtcgctgcgg catcctcgcc agtataaatg gcggttcttt tgaaaacatg aatgtcggtt
     3781 cagaccgtgt ttttacccag cttgcctgct gtctttcggc aagttcacag gcttcatcat
     3841 agttatctgc caaacaaagc acggatggac ggtcccacgc cccaccattc agacaataaa
     3901 ctacaatttt cccgtcaggt tgtgtaaccc catagggatg gtcccaccag gcgtccaact
     3961 gagctttaga gcgtttctcg ttaggagtgc agtcaaaatt cttgggcaat acaggatcga
     4021 gaggaatgcg attaggcata gctaattcct tattaactga ttggcaacga ggttacgctg
     4081 atccgttggt gatgaatagt agcaaagcgc acaaaatcat ctgcggtggt tgatgtacat
     4141 aacgcgtttg caccaaaggt gtctctttaa tgtatactgt ataaatgaac agtattattg
     4201 aggtgaaaac gctatgggct tcccttctcc tgcggcggat tatgttgaaa gccgaatttc
     4261 tcttgatcag cagataatta gacatccatc agcaacctac ttcatgcggg cagctgatag
     4321 ccatcaccgt gagggaatat tgcagggtgc tttgctggtg gttgattctt cgcttactcc
     4381 agttgatggt tcgctgcttg tgtgcgctat ggagggtgaa tatcgcataa agagatacag
     4441 gaagtatccg cgccagcacc tggaggattt aagcaccggg aagaaagagg cgttaccagt
     4501 agatgacgat agttacacgg gtagtaatgc tgtttttggt gtgatcactc atgtcatcaa
     4561 tgatgcccga agtggggaat ttgatgattg tccggtcatt taagctgcaa agtgctggtg
     4621 ctttatgcct gcgaagttta taattgtgta cacataacga gtacatgagg tgtttatgca
     4681 atccattaac ttccgtaccg cgcgcggcaa actttctgaa gtgctcaaca atgttgaggc
     4741 cggggaagag gttgaaatca cccgcagagg ccgtgagcca gcagtaattg tcagcaaggc
     4801 tactttcgaa gcctacaaaa aagcggcgct ggatgctgaa tttgcatccc tgtttgacac
     4861 cctggactcc accaacaagg aactggttaa ccgataatga ggcatatatc accggaagaa
     4921 cttattgcgc ttcatgatgc gaatataagc cgctacggcg gcctgccggg aatgtcagat
     4981 ccgggtaggg cagaggccat tatcgggaga gttcaggcca gagttgccta cgaagagatc
     5041 accgaccttt tcgaagtctc cgccacctac ctggtggcta cagcgagagg gcatatattc
     5101 aatgatgcca ataagcgtac cgcgctaaac agtgcactgt tatttctacg ccgtaacggg
     5161 gtgcaggtat ttgattcacc tgaactggca gaccttactg taggcgctgc gactggcgag
     5221 atatctgtat cttctgtcgc cgacacgtta cgtagattgt atggttctgc ggagtagatt
     5281 aatggcacgc aaata
//
LOCUS       DAMZFT010000235         5274 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.235, whole genome shotgun sequence.
ACCESSION   DAMZFT010000235 DAMZFT010000000
VERSION     DAMZFT010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5274)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5274)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5274
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.235"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            472..1041
                     /locus_tag="PN977_004986"
     CDS             472..1041
                     /locus_tag="PN977_004986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus Gp2 family protein"
                     /protein_id="HDI6044902.1"
                     /translation="MTYKYNPFWQQRIRETVRHALNVHPRLTALRVDLRFPDVPAATD
                     AAVISRFINALKARIDAYQKRKHREGKRVHPTTLHYVWAREFGECKGKKHYHLMLLVN
                     RDTWCRAGDYRAPGSLAGMIKQAWCSALGVDVGCHATLVHFPAWPAVWLERDDDTGFQ
                     QVLERADYLAKEHTKAHCTGERNFGCSRS"
     gene            1301..1702
                     /locus_tag="PN977_004987"
     CDS             1301..1702
                     /locus_tag="PN977_004987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004020115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6044903.1"
                     /translation="MYAKSFIALDGNGRLTGARTAQAAPYANYTCHLCGSALRYHPQY
                     DTELPWFEHTDDRLTEHGQQCPYVRPERREIQLIKRLQQFVPDALPVVRKASWHCRQC
                     HHDYYGERYCTHCQTGGFSIPRTTQEEICEF"
     gene            1690..2205
                     /locus_tag="PN977_004988"
     CDS             1690..2205
                     /locus_tag="PN977_004988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001386879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HDI6044904.1"
                     /translation="MRILNCYMANDSKGHFVTAKEAAKHNRQDVLCCVSCGCPLTLQR
                     GNDGQPPWFEHDQMTVAEKILLRCTWLDPAEKEARRLHLQGMTVPDYTVKVRKWFCVM
                     CDENYEGEKCCPSCGTGVYSREGGRRATGRIGTERFSRGNQLQSCNDISGSAALVGAS
                     RDREILALCPE"
     gene            complement(2307..2879)
                     /locus_tag="PN977_004989"
     CDS             complement(2307..2879)
                     /locus_tag="PN977_004989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016234457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDI6044905.1"
                     /translation="MLIGYIRVSTNDQNTDLQRNALMCAGCEQIFEDKMSGTKSERPG
                     LKRALKCLKRGDTLVVWKLDRLGRRMKHLIALTEELRAKGVNFRSLTDSIDTSTPMGR
                     FFFHMMGALAEMERELIVERTLAGLAAARAQGRVGGRRPKLTKEQHEQIARLLQKGYD
                     RKRLAIIYDIGLSTIYRYHPVGTVITEPEM"
     gene            2951..3400
                     /locus_tag="PN977_004990"
     CDS             2951..3400
                     /locus_tag="PN977_004990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6044906.1"
                     /translation="MGEAAKRNVGNGENQIPDMSFWTVTGGNGNFVIRQPDGLITQMV
                     TVSISGPVAMNGMTDNAYAITGSNKSYIATATLPFVFPNKVLGVIPLVSTTAYGGVSS
                     NITGSYATAVCSFAAVRGNNTIVFKVDKPLNAAFPSDSSVSALIIGR"
     gene            3403..3846
                     /locus_tag="PN977_004991"
     CDS             3403..3846
                     /locus_tag="PN977_004991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001505175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HDI6044907.1"
                     /translation="MNSVFFSPGSKSFYLQELFPEYEDAGTLPDDVIEITRETYEQFL
                     GLHPEGKEIGADSSGRPIWINSPPPSKEDEVLMAEMKKISLVSEANTYINTHQWPGKA
                     AIGRLKGNELEQYNLWLDYLDALELVDPSSAPDIEWPTPPAVQAR"
     gene            complement(3818..4420)
                     /locus_tag="PN977_004992"
     CDS             complement(3818..4420)
                     /locus_tag="PN977_004992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HDI6044908.1"
                     /translation="MNKAVLNNELIAIKAGDITIYNYDGETREYISTSTEYLAVGVGI
                     PACSCLDAPVTHKAGYAICRSADFNSWEYVPDHRGEIIYSTETGESKEITAPGDYPEN
                     TTTIAPLTPYDKWDGEKWVTDTEAQHSAAVDAAEAQRQSLIDAAMASISLIQLKLQAG
                     RKLTQAETTRLNAVLDYIDAVTATDTSTAPDVIWPELPEA"
     gene            complement(4420..>5274)
                     /locus_tag="PN977_004993"
     CDS             complement(4420..>5274)
                     /locus_tag="PN977_004993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001696582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber protein"
                     /protein_id="HDI6044909.1"
                     /translation="TSETLAATPKAVKAAYDLAAGKAPASHTHPWSQITGVPAASLTA
                     KGTVQLSSAINSTSEILAATPKAVKAAYDLANGKQPADATLTALAGLATAADRLPYFT
                     GADRAALATLTAIGRAIIAKGSIKDVLNYLGLGEGSALPVGVPVPWPSATPPTGWLKC
                     NGAAFSAEEYPELAKAYPTNKLPDLRGEFIRGWDDGRGIDTNRSLLSSQGDAIRNIIG
                     ALVDVRFNTYPSDSGVFTTSVIGDASSDSIKGGYAKRVTFDASRVVPTANENRPRNIA
                     FNYIVRAA"
BASE COUNT         1288 a         1298 c         1337 g         1351 t
ORIGIN      
        1 aaggagaacc tgtcggttac gcttaatata cccggtacac tgtcagctca ggggatacat
       61 gaaccgttat ctcaccagct acacagtata tacacactga cgctgacagt agtcagcagg
      121 accacaataa tcagtgccag cagtatggta acgatacggt ctgtattccg gtaatagccg
      181 aaggggtcga cagaaccaca atacgggcaa cacactgtat tatgagtaat cgcgtttcca
      241 cagtccctgc acttcttttt gcgtatcacc atcactccct cctcacagcc ttacccgtaa
      301 ccggtttttt acattaaaaa aacaacgctg aaaattcaac cgtcagtccg gagacgaccg
      361 ttcgggttat cacagagtgc ctgagacagt gtcctgccgg aggtcacttc agggactctg
      421 cgcatttttt acgacgtggc tattccgtat gaaccatacg gagatttaac catgacctac
      481 aaatacaacc ccttctggca gcaacgtatt cgtgagacgg tgcggcacgc actgaatgtt
      541 catccccgcc tgacggcatt gcgggttgac ctgcgtttcc cggatgtacc ggcagcaacg
      601 gacgcagctg tgatatcccg cttcatcaat gccctgaaag cccgaatcga cgcttaccag
      661 aaacgtaagc atcgggaagg taaacgcgtg catcccacaa ccctgcatta cgtctgggcc
      721 cgggagtttg gggagtgcaa aggtaaaaaa cactatcacc tgatgctgct ggttaaccgg
      781 gatacctggt gtcgtgccgg tgattaccgt gctccgggat cactggccgg gatgattaaa
      841 caggcgtggt gcagtgccct gggagtggat gtcgggtgcc atgccacgct ggtgcatttt
      901 ccggcctggc cggcggtgtg gctggaacgc gatgatgaca ctggctttca gcaggtgctg
      961 gaacgtgctg actatctggc gaaggaacat accaaagctc actgcaccgg tgagcgcaat
     1021 tttggctgta gccggagttg agcacgactg gcgcgccaat acccggcaaa ccaaaagtat
     1081 gggttgttgt ctggcggcaa cgatgtatcc actcgcccac tgactgtttt ttacgtcagt
     1141 gttccattgt gtgacaacca cagtgctgta acagcaacca gcctgcttta cccaaactcc
     1201 agtccagatt ttaactgaac cgccattcac accctgacca cgctgcccgt accgggacgg
     1261 ctgttgcctg cgtgtcgtct ggcggctgag gaatatgact atgtacgcaa aatcctttat
     1321 cgctcttgat ggcaacggac gtctgacggg cgcccgtact gcacaggccg caccttatgc
     1381 taactacacc tgccacttgt gtggcagtgc actcagatac catccgcaat acgacactga
     1441 acttccctgg tttgaacaca ctgacgacag gctgacagag cacggtcaac agtgccctta
     1501 tgtcaggccg gagcgcagag aaatacagtt gattaaacgt ctgcagcaat tcgtaccgga
     1561 tgccttaccc gtggtgcgta aagccagctg gcactgcaga caatgtcacc acgattatta
     1621 tggggagcgg tactgcacac actgccagac cggaggattc agtattcccc ggacaactca
     1681 ggaggaaata tgcgaattct gaactgttat atggcgaatg acagcaaagg ccattttgtt
     1741 acggcgaaag aagcagcgaa gcacaaccgc caggacgttt tgtgctgtgt gtcctgtgga
     1801 tgcccgttaa cacttcagcg gggcaatgac ggacaaccac cgtggtttga acatgaccag
     1861 atgactgtcg ctgagaaaat cctgctgcga tgcacctggc ttgacccggc agagaaagag
     1921 gcccgtcgtt tgcatctgca gggtatgacg gttccggatt atacggtgaa ggtgagaaag
     1981 tggttttgtg tgatgtgtga cgaaaattat gagggggaaa agtgctgccc gagttgtggt
     2041 accggggtat acagcaggga ggggggcagg agggcaactg gaaggatagg aactgagcgg
     2101 ttcagtcgcg gaaatcagtt gcagagctgt aatgatatct ccgggagtgc tgcgttggta
     2161 ggggcatccc gggatagaga aattttagca ctttgccctg aatgattgta gacgtcccga
     2221 gtactcgttg atgcgccgcg cgataaatgc gctctatctt gtgcgataaa tggcatccac
     2281 gcggcgtttc attatttgaa aaagaattac atttcaggtt ccgttatgac agtcccaaca
     2341 gggtgatagc ggtagatcgt tgacagtcca atatcataga taattgccag ccgctttctg
     2401 tcataccctt tctggagcaa tcttgcgatc tgctcatgct gctccttcgt cagtttcggg
     2461 cgacgtcctc caactctccc ttgtgctcgc gcagcagcca gtcctgccag cgtgcgctca
     2521 actatcagtt cacgctccat ttctgccagt gcacccatca tatgaaaaaa gaaccttccc
     2581 atcggagtgc tggtatcgat tgaatccgtc agactgcgaa aattgacacc ttttgcgcgt
     2641 agctcttctg tgagagcaat gaggtgtttc attcttctac ccaaccgatc cagcttccag
     2701 accaccaatg tatcccctct tttaaggcac tttaaagcgc gtttcaggcc tggtcgttcc
     2761 gacttggttc cgctcatttt gtcctcaaaa atctgttcac atcctgcgca cattaatgca
     2821 ttgcgttgca aatctgtgtt ttggtcattt gttgacacac gtatgtagcc aatcagcatt
     2881 aaaaatactc ccttttgagt ggttaaagta ctgagataat gtgaggagga tttgagagat
     2941 aaacgttggt ttgggagaag cggcaaaacg gaatgtgggc aacggggaaa accaaatccc
     3001 tgatatgtct ttctggacgg ttactggtgg caatggaaat tttgtgattc gtcaacctga
     3061 cgggctaatc actcagatgg ttactgtaag tataagcggt ccagtggcga tgaatggaat
     3121 gactgataat gcttatgcca ttacaggttc taataagtct tatattgcca cagccacatt
     3181 gccctttgta tttcctaata aggtgctggg cgttatccct ctggtatcaa caacagccta
     3241 tggtggtgta tccagtaata ttacaggttc atacgcgacg gcggtttgtt cttttgccgc
     3301 tgtccggggg aataatacga ttgtattcaa agtcgacaaa ccactgaatg cagcctttcc
     3361 ttcagatagc agcgtctcag cgttaatcat tggacggtaa taatgaactc agtattcttt
     3421 tcacccggaa gtaaaagttt ttatctacaa gaattgtttc cagaatatga ggatgcggga
     3481 acgcttccag atgatgttat tgaaattacc cgagaaacat atgagcaatt tcttgggctg
     3541 catccagaag ggaaagaaat tggcgctgac agttcaggac ggccaatatg gattaattcc
     3601 ccaccgcctt caaaagagga tgaggtgttg atggctgaaa tgaaaaaaat atctttggtt
     3661 tcagaagcca atacttacat caatacccat cagtggcctg gcaaagctgc tattggtcgt
     3721 ctgaaaggta acgagctgga gcaatataat ttatggctgg attatctgga cgcactggaa
     3781 ctggttgatc cctccagtgc tccagatatt gaatggccta cgcctccggc agttcaggcc
     3841 agatgacgtc cggcgctgtg ctggtatctg ttgccgtcac cgcgtcaatg taatccagca
     3901 cggcgttaag tcgggttgtt tctgcctgcg tcagtttccg tccggcctgt aatttcagct
     3961 gaatcagact aatggaagcc attgctgcat caatcagtga ctggcgctgt gcttctgctg
     4021 cgtctactgc ggcactatgc tgtgcctcag tatccgtcac ccatttctca ccatcccatt
     4081 tatcatatgg cgttaacggg gcgatagtgg ttgtattttc agggtaatca cccggagctg
     4141 tgatttcttt cgattctcct gtttcagtgc tatagatgat ttcaccgcga tggtctggca
     4201 catattccca tgagttaaaa tctgcagaac ggcagattgc ataaccagct ttatgtgtaa
     4261 ctggtgcatc taaacaagaa catgccggga taccgacgcc aaccgcaaga tattcagttg
     4321 atgtggaaat atattcccgc gtttcaccat cataattata aatggtaatg tctcccgctt
     4381 ttatggcaat gagttcgtta tttaatacgg ctttattcat caggcagccc tcacgatata
     4441 attaaaggca atgttacgag gtcggttttc gtttgcagtt ggaacaactc tggaagcatc
     4501 gaatgttact cgctttgcat aaccaccttt aattgaatca gatgaagcat ctccgatgac
     4561 gctggttgta aaaacgccag aatcagaagg gtaggtatta aacctgacat ccactaatgc
     4621 accaattata tttcgaatgg catctccctg tgacgaaagc aagctacggt tagtatctat
     4681 tcctcttccg tcatcccagc cacgaatgaa ctcaccacgt aaatcaggca atttatttgt
     4741 cgggtaagcc tttgccagtt ccgggtattc ttcagcagaa aaagccgcac cgttgcattt
     4801 cagccagcct gttggcggag tggcggaagg ccacggaaca ggcacgccaa cgggtaatgc
     4861 tgaaccttct cccaaaccaa ggtaattgag gacatctttt atactgccct tagcgataat
     4921 agcgcgacca atagctgtaa gggttgccag cgctgcgcgg tctgctccgg taaaataagg
     4981 caaccgatct gctgcagtgg caagtcctgc cagagcagtg agcgtggcat ctgccggttg
     5041 ttttccgttt gccagatcgt atgcagcctt gaccgctttc ggtgtggcgg ccagtatttc
     5101 agacgtgctg ttgatggcac tgctcagttg tacggtgcct tttgccgtca gcgaggccgc
     5161 aggcactccc gttatctgac tccacgggtg agtgtggctg gcaggcgcct tgccggctgc
     5221 caggtcgtat gcagccttga ccgctttcgg tgtggcggcc agcgtttcag acgt
//
LOCUS       DAMZFT010000236         5261 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.236, whole genome shotgun sequence.
ACCESSION   DAMZFT010000236 DAMZFT010000000
VERSION     DAMZFT010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5261)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5261)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5261
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.236"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(<1..215)
                     /gene="osmE"
                     /locus_tag="PN977_004994"
     CDS             complement(<1..215)
                     /gene="osmE"
                     /locus_tag="PN977_004994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmE"
                     /protein_id="HDI6044910.1"
                     /translation="MNKNMAGILSAAAVLTMLAGCTAYDRTKDQFVQPVVKDVKKGMS
                     RAQVAQIAGKPSSEVSMIHARGTCQTY"
     gene            417..1244
                     /gene="nadE"
                     /locus_tag="PN977_004995"
     CDS             417..1244
                     /gene="nadE"
                     /locus_tag="PN977_004995"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HDI6044911.1"
                     /translation="MTLQQQIIKALGAKPQINAEEEIRRSVDFLKSYLQTYPFIKSLV
                     LGISGGQDSTLAGKLCQMAINELRQETGNESLQFIAVRLPYGVQADEQDCQDAIAFIQ
                     PDRVLTVNIKGAVLASEQALREAGIELSDFVRGNEKARERMKAQYSIAGMTSGVVVGT
                     DHAAEAITGFFTKYGDGGTDINPLYRLNKRQGKQLLAALGCPEHLYKKAPTADLEDDR
                     PSLPDEVALGVTYDNIDDYLEGKNLPEQVARTIENWYLKTEHKRRPPITVFDDFWKK"
     gene            1474..2361
                     /gene="cho"
                     /locus_tag="PN977_004996"
     CDS             1474..2361
                     /gene="cho"
                     /locus_tag="PN977_004996"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease Cho"
                     /protein_id="HDI6044912.1"
                     /translation="MVRRLTSPRLEFEAAAIYEYPEHLRSFLNDLPTRPGVYLFHGES
                     DTMPLYIGKSINIRSRVLSHLRTPDEAAMLRQSRRISWICTAGEIGALLLEARLIKEQ
                     QPLFNKRLRRNRQLCALQLNEKRVDVVYAKEVDFSRAPNLFGLFANRRAALQALQTIA
                     DEQKLCYGLLGLEPLSRGRACFRSALKRCAGACCGKESHDDHALRLRQSLERLRVVCW
                     PWQGAVALKEQHPEMTQYHIIQNWLWLGAVNSLEEATMLIRTPAGFDHDGYKILCKPL
                     LSGNYEITELDPANDQRAS"
     gene            complement(2321..2896)
                     /gene="ves"
                     /locus_tag="PN977_004997"
     CDS             complement(2321..2896)
                     /gene="ves"
                     /locus_tag="PN977_004997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310475.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="environmental stress-induced protein Ves"
                     /protein_id="HDI6044913.1"
                     /translation="MEYFDMRKMSVNLWRNAAGETREICTFPPAKRDFYWRASITSIA
                     ANGEFSLFPGMERIVTLLEGGEMFLESADRFNHTLKPLQPFAFAADQVVKAKLTAGQM
                     SMDFNIMTRLDVCKAKVRIAERTFTTFGSRGGVVFVINGAWQLGDKLLTTDQGACWFD
                     GRHTLRLLQPQGKLLFSEINWLAGHSPDQVQ"
     gene            complement(3099..3584)
                     /gene="spy"
                     /locus_tag="PN977_004998"
     CDS             complement(3099..3584)
                     /gene="spy"
                     /locus_tag="PN977_004998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-independent periplasmic protein-refolding
                     chaperone"
                     /protein_id="HDI6044914.1"
                     /translation="MRKLTALFVASTLALGAANLAHAADTTTSAPADAKPMMHHKGKF
                     GPHQDMMFKDLNLTDAQKQQIREIMKGQRDQMKRPPLEERRAMHDIIASDTFDKAKAE
                     AQIAKMEEQRKANMLAHMETQNKIYNILTPEQKKQFNANFEKRLTERPAAKGKMPATA
                     E"
     gene            complement(3914..4882)
                     /gene="astE"
                     /locus_tag="PN977_004999"
     CDS             complement(3914..4882)
                     /gene="astE"
                     /locus_tag="PN977_004999"
                     /EC_number="3.5.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase"
                     /protein_id="HDI6044915.1"
                     /translation="MDNFLALTLTGKKPVITEREINGVRWRWLGDGVLELTPLTPPQG
                     ALVISAGIHGNETAPVEMLDALLGAISHGEIPLRWRLLVILGNPPALKQGKRYCHSDM
                     NRMFGGRWQLFAESGETCRARELEQCLEDFYDQGKESVRWHLDLHTAIRGSLHPQFGV
                     LPQRDIPWDEKFLTWLGAAGLEALVFHQEPGGTFTHFSARHFGALACTLELGKALPFG
                     QNDLRQFAVTASAIAALLSGESVGIVRTPPLRYRVVSQITRHSPSFEMHMANDTLNFM
                     PFEKGTLLAQDGEERFTVTHDVEYVLFPNPLVALGLRAGLMLEKIS"
     gene            complement(4875..>5261)
                     /locus_tag="PN977_005000"
     CDS             complement(4875..>5261)
                     /locus_tag="PN977_005000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-succinylarginine dihydrolase"
                     /protein_id="HDI6044916.1"
                     /translation="VLPQECREHAGVWGYLNELLVADNPISELKVFDLRESMANGGGP
                     ACLRLRVVLTEEERRAVNPAVMMNDTLFNALNDWVDRYYRDRLTAADLADPQLLREGR
                     EALDVLSQLLNLGSVYPFQREGGGNG"
BASE COUNT         1306 a         1344 c         1270 g         1341 t
ORIGIN      
        1 atgtaggtct ggcaagtacc acgagcatgg atcatgctca cttcagacga aggtttaccc
       61 gcaatttgtg caacctgcgc ccggctcatg ccttttttga cgtctttcac cacaggctgt
      121 acaaactggt ctttggtacg atcataagcc gtacaacccg ccagcatggt taataccgcc
      181 gctgcactca gaattcctgc catattcttg ttcatattcc gtcctcttgt ttatcagcgt
      241 gttagataag cctggaatac attgggcgct ttttcaagcc cgtgaacgaa acggctccgc
      301 tttcagagga ttcctgtatg acgttttaac caccattcag cccgctgtcg cttgtcgttt
      361 cagtagcaac gggttagctt taaggaagtt ttgtcttttc tgtctggagg ggttcaatga
      421 cattgcaaca acaaataata aaggcgctgg gcgcaaaacc gcagattaat gctgaagagg
      481 aaattcgtcg tagtgtcgat tttctgaaaa gctacctgca aacttatccg ttcattaaat
      541 cactggtgct aggtatcagc ggcggtcagg actccacgct tgccggtaag ctgtgccaga
      601 tggcgattaa tgaactgcgc caggagaccg gcaacgaatc actgcaattt attgccgtac
      661 gcctgcccta tggtgttcag gccgacgaac aagattgcca ggatgccatt gcctttattc
      721 aaccggatcg cgtattaacc gttaatatca agggcgcggt attggccagc gaacaggcgc
      781 tgcgcgaagc gggtattgaa ttaagtgatt ttgtccgtgg caatgaaaaa gcgcgtgagc
      841 ggatgaaagc acaatatagc attgcgggta tgaccagcgg tgtcgtggtg ggcaccgatc
      901 atgcagcaga agccattacc ggattcttca ctaaatatgg tgacggcggt acggacatta
      961 atccactgta tcgtctcaac aaacgtcagg gtaaacagtt actggcagca ttaggttgcc
     1021 cggaacacct ttataagaaa gccccaactg ccgatctgga agatgatcgc ccttctctgc
     1081 cagatgaagt ggcactcggc gtgacctatg acaatatcga cgactatctg gaagggaaaa
     1141 acctacctga acaggtcgcc agaacaatag agaactggta tctgaaaact gaacataaac
     1201 gccgtccgcc aattaccgtt ttcgatgatt tctggaagaa gtaataattt gcacatattg
     1261 gtttgtgcga aaaagagtac tttgttcacg ccggatgcgg cgtgaacgcc ttattcgacc
     1321 tacaaaacta tgcaaattca atatattgca ggagtcgaat aggcctgaca ggcgtagcac
     1381 gtcagacggt gtaacctttg tcatcgaccc gcctcttttt taatcgctct ccgcctgtta
     1441 cactggatag ataaccagca ttcggagtca acagtggtac ggcgtttaac ttctccgcgg
     1501 ctcgaatttg aagctgcggc aatttatgaa tatcccgaac atttacgttc attccttaat
     1561 gacttaccca cccgaccagg ggtgtatctg tttcatggcg aaagtgacac catgccgctc
     1621 tatatcggca aaagcattaa catccgcagt cgggtgcttt ctcatttacg cacccctgat
     1681 gaagccgcca tgctacggca atcccgacgc atcagctgga tatgtaccgc cggtgaaatc
     1741 ggcgctctgc tccttgaagc gcgattaatc aaagaacaac agccactgtt taataaacgc
     1801 ttgcggcgaa atcgccagct ctgtgccttg caattaaatg aaaagcgcgt cgatgtggtg
     1861 tatgccaaag aggtggattt ttcacgagcc cccaacctgt ttggcctgtt tgccaatagg
     1921 cgcgcagctt tgcaagcatt gcagaccatc gctgatgaac aaaaactttg ttatggtctg
     1981 ttgggactgg agccgttaag tcgcggtcgt gcatgttttc gttcagcgct aaaacgttgc
     2041 gccggagcat gctgcggtaa agagagccat gacgatcatg cgctacgttt gcgccagtct
     2101 ctggagcgtt tgcgggtggt gtgttggcct tggcaagggg cggtggcgct gaaagaacag
     2161 cacccggaaa tgactcaata tcatattatt caaaactggc tgtggctggg ggcggttaat
     2221 tcgctggaag aagcgacaat gttaattcgg acacccgccg ggtttgatca cgacggttat
     2281 aaaattcttt gtaagccgct gctttccggt aactatgaaa ttactgaact tgatccggcg
     2341 aatgaccagc gagccagttg atttcactga acaacagttt accttgcggt tgtaaaagcc
     2401 gcagggtgtg cctgccatcg aaccagcacg cgccctggtc ggtagtgagc aatttatcgc
     2461 ccagttgcca cgccccgtta atcacaaaca ccaccccgcc gcgtgagcca aaggtagtaa
     2521 acgtacgttc ggcaattctg actttcgcct tacagacatc caggcgtgtc atgatgttga
     2581 aatccatcga catctgccct gccgtcagtt tcgctttaac cacctggtcc gccgcaaagg
     2641 caaaaggctg taacggtttt aaggtatggt taaagcggtc tgcgctttca aggaacatct
     2701 cgccgccttc cagcaacgtc actatccttt ccatacctgg aaacaaagaa aactccccat
     2761 tcgccgcgat ggatgtaatg ctggcacgcc agtaaaaatc acgttttgcc ggtgggaaag
     2821 tgcaaatttc gcgcgtttca ccggcagcgt ttcgccacag attcaccgac attttacgca
     2881 tatcaaagta ttccatactc gctcccggcc cgttcttttc attgttatcg ttcaggtaac
     2941 cgtggatggt tatcggcaac tcgggtgatt tacgttagtg gcaatcaggc tttttttgca
     3001 tctgtttgag aacaaaaaag aaaaccgccg atcctgtcca ccgcattact gcaaggtagt
     3061 ggacaagacc ggcggtctta agttttttgg ctgaaagatt attcagcagt tgcaggcatt
     3121 ttaccttttg ccgctggacg ttctgtcaga cgcttctcaa aattagcatt aaattgcttt
     3181 ttctgttccg gcgtcaggat gttgtaaatc ttgttctggg tttccatgtg cgccagcatg
     3241 ttagctttgc gctgttcttc catttttgcg atctgcgctt cagcttttgc tttatcgaag
     3301 gtatcgctgg caatgatgtc atgcattgcg cggcgttctt ccagcggcgg acgtttcatc
     3361 tggtcacgct ggcctttcat gatttcgcgg atctgctgtt tctgcgcgtc ggtcaggttc
     3421 aggtctttga acatcatgtc ctgatgcgga ccgaacttgc ctttgtggtg catcatcggc
     3481 ttcgcgtcag ccggtgctga ggtagtggtg tctgcggcat gggccaggtt agccgcgcca
     3541 agagccaggg tagaggcaac aaacagtgca gttaatttac gcatattcta tatccttcct
     3601 ttcagttatt tattacggct ttcttaagta gcgtgccgtg ttgacgagat taactttact
     3661 gactttagcg tcaattaatc agagcaacgg taaaacaatg aaagtgtaaa aaacactttt
     3721 tcgccaatta tggagaaaaa aagaaaattt gatggagagt aatgagagaa tattacaaca
     3781 cgatgatttt gcagagatta tgaagaacta taccggatga ctggtgataa ataaagcaaa
     3841 taaccaggat taatctgtat taatttataa gaaagtaact taatacccgc agaatgattt
     3901 ctgcgggtaa gtattagctt attttttcga gcattaatcc cgcacgtaat cccaacgcta
     3961 ccaacggatt agggaataac acatactcta catcatgggt tacggtaaaa cgttcctccc
     4021 cgtcctgcgc cagcaatgtt cctttctcaa acggcataaa attcagcgtg tcatttgcca
     4081 tatgcatttc gaaggacggc gagtggcgag taatttgcga aaccacccga taacggagcg
     4141 gcggtgttct cacgataccg acactctcac cagatagcag cgcagcaatt gcgctggcag
     4201 ttactgcaaa ctggcgaaga tcgttttgcc caaagggcaa cgctttgcca agttccagcg
     4261 tacaggccag cgcgccaaaa tgtctggcgc tgaaatgggt aaacgtacca ccaggctcct
     4321 gatggaacac cagtgcctcc agccccgccg cacccagcca cgtcagaaat ttctcgtccc
     4381 agggaatgtc gcgttgcggt aatacaccga actgcggatg caaggagcca cgaattgcgg
     4441 tatgtagatc aaggtgccag cgcacagatt ctttgccctg gtcataaaaa tcttccaggc
     4501 actgttccag ttcgcgcgcc cgacaggttt ccccgctttc agcaaatagc tgccaacgac
     4561 cgccaaacat tcggttcata tcgctatggc aataacgttt cccttgcttc agcgcaggag
     4621 gattcccgag gatcaccagc aaccgccaac gtaacgggat ctcgccgtga gatatcgcgc
     4681 caagcaacgc atccagcatt tccacaggtg ccgtctcatt accgtgtatt cccgctgaaa
     4741 tcaccagtgc gccttgcggt ggcgttaatg gcgtcagttc cagcacgcca tcgcccagcc
     4801 agcgccagcg aacgccgttg atttctcgct cggtgataac cggtttttta cccgttaatg
     4861 tcagagcaag aaaattatcc attgccccct ccctcgcgct ggaacggata aaccgaaccg
     4921 agattcagta attggctcaa tacatccagt gcttcccgcc cttcgcgcag caattgcggg
     4981 tcggccagat cggcagcagt aaggcgatcg cggtagtaac gatccaccca gtcattgagc
     5041 gcattaaaca gcgtatcgtt catcatcacc gccggattca ctgcccggcg ttcttcttct
     5101 gtcaatacca cgcgcaaccg caggcacgcc ggacctccgc cattcgccat gctttcacgt
     5161 aaatcaaaga cttttagttc gctaatcggg ttgtcagcga caaggagttc attgagataa
     5221 ccccatactc cggcgtgttc ccgacactcc tgaggcagca c
//
LOCUS       DAMZFT010000237         5179 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.237, whole genome shotgun sequence.
ACCESSION   DAMZFT010000237 DAMZFT010000000
VERSION     DAMZFT010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5179)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5179)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5179
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.237"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..373
                     /locus_tag="PN977_005001"
     CDS             <1..373
                     /locus_tag="PN977_005001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SPASM domain-containing protein"
                     /protein_id="HDI6044917.1"
                     /translation="CKDNLVVESNGDIYECDHFVYPQYKIGNINKSELKTMNSVQLTA
                     QKKRISAKCQQCAYKPICNGGCPKHRITKVNNETVSYFCEGYKILFSTMVPYMNAMVE
                     LAKNRVPLYHIMDVAKQMENN"
     gene            complement(339..464)
                     /locus_tag="PN977_005002"
     CDS             complement(339..464)
                     /locus_tag="PN977_005002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6044918.1"
                     /translation="MKTKHPTGIYVGCLVKVIRRRLRMELRGSVINYSPFVLQHP"
     gene            664..2349
                     /locus_tag="PN977_005003"
     CDS             664..2349
                     /locus_tag="PN977_005003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HDI6044919.1"
                     /translation="MITIPITFCMLIAKYLCLLKPFWLRKNNKTSVLLIIIILAMILG
                     VVKIQVWLNDWNNDFFNALSQKETDKLWQLVLWFPALLGIFVLISVNKTWLIKLLTIR
                     WREWLTDYYLNRWFADKNYYFTQIYGEHKNTDNPDQRIAEDILLLISKTLSLSFGFIQ
                     SLSMLITFTVILWQSAGTLSFTVGGTEWNIQGYMVYTVVLIVIGGTLFTHKVGKRIRP
                     LNVEKQRSEATFRTNLVQHNKQAELIALSNAESLQRQELSENFHTIKENWHRLMNRQR
                     WLDYWQNIYSRSLSVLPYFLLLPQFISGQINLGGLMKSRQAFMLVSNNLSWFIYKYDE
                     LAVLAAVIDRLYEFHQLTEQRPTNKPKNCQHAVQVADASIRTPDNKIILENLNFHVLP
                     GKWLLLKGYSGTGKTTLLKTLSHCWPWFKGDISSPADSWYVSQTPLIKTGLLKEIICK
                     ALPLSVDDKSLSEVLHQVGLGKLATRIHDHDRWGDILSSGEKQRIALARLILRRPKWI
                     FLDETTSHLEEQEAIRLLRLVREKLPTSGVIMVTHQPGVWNLADDICDISAVL"
     gene            2387..4759
                     /locus_tag="PN977_005004"
     CDS             2387..4759
                     /locus_tag="PN977_005004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HDI6044920.1"
                     /translation="MKRVLIPGVILCGADVAQAVDDKNMYMHVFEEMTVYAPVPVPVN
                     GNTHYTSESIERLPTGNGNISDLLRTNPAVRMDSTQSTSLNQGDIRPEKISIHGASPY
                     QNAYLIDGISATNNLNPANESDASSATNISGMSQGYYLDVSLLDNVTLYDSFVPVEFG
                     RFNGGVIDAKIKRFNADDSSVKLGYRTTRSDWLTSHIDENNKSAFNQGSSGSTYYSPD
                     FKKNFYTLSFNQELADNFGVTAGLSRRQSDITRADYVSNDGIVAGRAQYKNVIDTALS
                     KFTWFASDRFTHDLTLKYTGSSRDYNTSTFPESDREMGNKSYGLAWDMDTQLAWAKLR
                     TTVGWDHISDYTRHDHDIWYTELSCTYGDITGRCTRGGLGHISQAVDNYTFKTRLDWQ
                     KFTVGNVSHQPYFGAEYIYSDAWTERHNQSESYVINAAGKKTNHTIYHKGKGSLGIDN
                     YTLYMADRISWRNVSLMPGVRYDYDNYLSNHNISPRFMTEWDIFADQTSMITAGYNRY
                     YGGNILDMGLRDIRNSWTESVSGNKTLTRYQDLKTPYNDELAMGLQQKIGKNVIARAN
                     YVYREAHDQISKSSRTDSATKTTITEYNNDGKTKTHSFNLSFELAEPLHISQVDINPQ
                     IVFSYIKSKGNLSLNNGYEESNTGDNQVVYNGNLVSYDSVPVADFNNPLKISLNMDFT
                     HQPSGLVWANTLTWQEARKARIILGKTNAQYISEYSDYKQYVDEKLDSSLTWDTRLSW
                     TPQFLKQQNLTFSADILNVLDSKTAVDTTNTGVATYASGRTFWLDVSMKF"
     gene            4816..>5179
                     /locus_tag="PN977_005005"
     CDS             4816..>5179
                     /locus_tag="PN977_005005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HDI6044921.1"
                     /translation="MRNLCFLLTLVATLLLPGRLIAAALPQDEKLITGQLDNGLRYLI
                     YPHAQPKDQVNLWLQIHTGSLQEEDNERGVAHFVEHMMFNGTKTWPGNKVIETFESMG
                     LRFGRDVNAYTSYDETVYQ"
BASE COUNT         1491 a         1131 c         1134 g         1423 t
ORIGIN      
        1 ctgcaaggat aatctggttg ttgaaagtaa tggagacatt tacgaatgcg accattttgt
       61 ctatccacag tacaaaattg gaaacattaa taaatctgaa ctcaaaacga tgaacagtgt
      121 tcaactgaca gcgcaaaaaa aacggatttc agcgaaatgt cagcaatgtg catataaacc
      181 tatctgcaat ggtggttgcc ctaagcatcg tattactaaa gtaaacaatg agactgtttc
      241 ctatttttgc gaaggttata aaatcctttt ttcaaccatg gtaccttata tgaacgccat
      301 ggtggagtta gctaagaaca gagtaccgct ttaccacatt atggatgttg caaaacaaat
      361 ggagaataat taataacgct cccccttagc tccattctta gcctccttct tatgacctta
      421 acaaggcacc cgacataaat tcctgtcggg tgtttcgtct tcatcatctt cctttgcgaa
      481 aatcctggct tacgaggtat gcgtcagggc attagcaata cccttgatta tcaatagcat
      541 aaccataacc actgaaaata aaatatttct ttatatttca tattgttaaa tgatacttaa
      601 ataaatttca tcagtatatc attgccatag ataatcattg gatggcacaa taaattttcg
      661 ctaatgataa ccattcccat tacgttttgt atgttgatag caaagtatct ctgtctgctg
      721 aagccctttt ggctgcgtaa gaataataaa acttcagtgt tgttaatcat tattatcctc
      781 gccatgatcc ttggcgtggt taaaattcag gtctggttaa acgactggaa taatgatttt
      841 ttcaatgcgc taagccagaa agaaactgat aagctttggc agcttgtcct ttggttccct
      901 gccctgctgg ggatctttgt attaatatcc gtaaacaaaa catggttaat taaactactg
      961 actattcgct ggcgcgaatg gttaacagat tactacctta atcgctggtt tgcagataaa
     1021 aactactact tcacgcagat ctacggcgag cataaaaata cagataaccc tgaccaacgt
     1081 atcgctgaag acattctctt actgatcagc aaaacgctca gtctttcatt tggcttcatc
     1141 cagtcactta gcatgctgat cacctttacc gttattcttt ggcaaagcgc gggtacgctc
     1201 tcattcactg ttggtggaac tgaatggaat atccaggggt atatggtcta taccgtcgtg
     1261 ctcattgtga tcggtggaac tttatttacc cataaggttg gtaaacgtat tcgtccgctt
     1321 aatgtggaaa aacaacgtag cgaagcgaca tttcgaacta atcttgtgca gcataataag
     1381 caggcagaac tgattgcgct aagcaatgct gagtctctac aacgccagga gttgagcgaa
     1441 aattttcata ccattaaaga gaactggcat cgtttaatga atcgtcagcg gtggcttgat
     1501 tactggcaga atatttattc gcgctcgctt agcgttcttc cctactttct gttattgccg
     1561 cagtttatta gcggacagat caatctgggc ggactgatga aatcgcgcca ggcatttatg
     1621 ctggtatcga acaatttaag ctggtttatt tataaatatg atgaacttgc tgtactggct
     1681 gcggttatcg atcgcttgta tgagttccat caactcaccg aacagcgccc tacgaataag
     1741 cctaaaaatt gccaacatgc ggtacaagtg gctgacgcga gtattcgtac tcctgataat
     1801 aagatcatat tagagaacct gaactttcat gttttgccag gcaaatggct attgctgaaa
     1861 ggttactctg gtacgggaaa aaccacactg cttaaaacat tatcccactg ctggccgtgg
     1921 tttaaaggtg atatttcttc tcctgctgac agttggtatg tgtcacaaac accgttaatc
     1981 aaaaccggct tactgaaaga gattatttgt aaagcacttc ccctgtccgt agacgataaa
     2041 tcgttgagtg aagtactgca tcaggttggg ctggggaaat tggctacgcg tattcatgac
     2101 cacgatcgct ggggagatat tctttccagc ggcgaaaaac aacgtatcgc cctggctcga
     2161 ttaattttac gacgtccgaa atggatattt cttgacgaaa ctacctctca tcttgaggaa
     2221 caagaggcaa tccgcttact gcgtttagtg cgagaaaaac tccccacaag cggcgtcatt
     2281 atggttacac atcaacccgg tgtctggaac ctggccgatg atatttgtga cattagcgcg
     2341 gttttataaa aacaataaaa gccctgctgt acggagatat aaataaatga agcgagttct
     2401 tattcctggc gtcattttat gtggcgctga tgtagcgcag gccgtcgatg acaaaaatat
     2461 gtacatgcat gtttttgaag agatgacggt ctatgctcct gtccctgtac ccgtaaacgg
     2521 caacacgcat tacaccagtg aaagcatcga gcgtttaccg accgggaatg gcaatatcag
     2581 cgatctgctg agaaccaacc ctgcggtacg catggattca acgcaaagta cctcattgaa
     2641 ccagggagat attcgcccgg agaaaatctc tattcacggt gcgtcgccct accagaatgc
     2701 ctatttgatt gacggtatta gtgcaacgaa taacctgaac ccagcgaatg agtccgatgc
     2761 cagtagtgca accaatatta gcgggatgtc acaggggtat tatcttgatg tcagcttact
     2821 ggacaatgtg acgctttatg acagttttgt gccggttgaa ttcggtcgct tcaatggcgg
     2881 ggtaattgat gcaaagatca aacgcttcaa cgctgatgat agcagcgtga aactgggtta
     2941 tcgcactacg cgttcggact ggttaacatc gcatatcgat gagaataaca agagcgcatt
     3001 taatcaaggt tcttcaggaa gtacttatta ctctccagat tttaaaaaga acttttatac
     3061 cttgtcgttt aatcaggaac tcgctgataa ctttggcgtt accgccggtt tatcgcgccg
     3121 ccagtctgat atcacccgcg cggattatgt ttcgaatgac ggcattgtcg ccggccgggc
     3181 acagtataaa aacgttatcg atactgcatt gagcaaattt acctggtttg ccagcgaccg
     3241 ctttacccac gatttaacct taaaatatac cggctccagc cgtgattata ataccagcac
     3301 cttcccggag tctgatcgcg aaatgggtaa taaatcctat ggtctggcat gggatatgga
     3361 tacccagctc gcatgggcca aactgcgtac caccgttggt tgggatcata ttagtgatta
     3421 tacccgtcac gatcatgaca tctggtacac cgaactttca tgtacatatg gtgatattac
     3481 agggcgttgc acccgtggcg gattaggaca catttcccag gctgtagata attacacctt
     3541 caaaacacgc ctggactggc aaaaattcac cgtgggtaat gtttcgcatc aaccctactt
     3601 cggcgcggaa tacatctatt ccgatgcatg gactgaacgc cataaccagt ctgaatccta
     3661 tgtgattaat gctgccggaa agaaaactaa ccataccatt taccataaag gtaaaggcag
     3721 cctgggaatt gacaactaca cgctgtatat ggcggatcgc attagctggc gtaatgtgtc
     3781 attaatgccc ggcgtgcggt atgactatga caactatctg tcaaaccaca atatctcccc
     3841 gcgctttatg acggaatggg atatttttgc tgatcaaacc tcaatgatta ccgcaggtta
     3901 taaccgttac tatggcggga atattcttga tatgggatta cgtgatatcc gcaatagctg
     3961 gacggaatcg gtatcaggca ataaaaccct gacgcgttat caggatttga aaacgcctta
     4021 taacgatgaa ctggcaatgg ggttgcagca gaaaatcggt aagaacgtta ttgcgcgtgc
     4081 aaactatgtt taccgtgaag cgcatgatca aatcagtaaa agcagtcgta ccgacagcgc
     4141 gactaaaacc accattactg aatataacaa cgacggtaaa accaaaacgc attcgttcaa
     4201 cctcagtttt gaactggccg aacccctgca tatcagccag gtagatatta acccgcaaat
     4261 tgtctttagc tatatcaaga gcaagggcaa cttgtcgtta aacaatggtt atgaggagag
     4321 caataccggt gataaccagg tggtttataa cggtaatctg gtctcttacg atagcgttcc
     4381 agtggcggat tttaataatc cattaaagat ctccttaaac atggatttca cgcatcaacc
     4441 gagcgggttg gtgtgggcga atacgctgac ctggcaagaa gcgcgtaaag ctcgcattat
     4501 cctgggtaag acgaatgcgc aatacatcag cgaatattca gattacaagc agtatgttga
     4561 cgaaaaactg gatagcagcc tgacctggga cacccgcttg tcctggacgc cacaatttct
     4621 gaaacaacaa aacctgacgt tcagtgccga tattctcaat gtactggata gcaaaaccgc
     4681 tgttgataca acgaataccg gtgtggcgac ctacgccagt ggccgtactt tctggcttga
     4741 tgtcagcatg aaattttaat agttcggatt ttacttaacc gggtaaattc gcccggtttt
     4801 cgcatggaga taattatgag aaacctctgt ttcttactga cgttagtggc aactctgttg
     4861 ctccccgggc gactgattgc cgccgcctta ccgcaggatg aaaagttaat taccgggcaa
     4921 ctggacaatg gcttgcgata tctgatttat ccgcatgctc aaccaaagga tcaggtaaat
     4981 ttatggctgc aaattcatac cggttcattg caggaagagg acaatgagcg cggcgtggct
     5041 cattttgtag aacatatgat gtttaacggc acaaaaacat ggccgggtaa taaagtcatc
     5101 gaaacatttg agtcaatggg ccttcgtttt ggtcgcgatg ttaatgccta taccagctat
     5161 gacgaaacag tgtatcagg
//
LOCUS       DAMZFT010000238         5051 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.238, whole genome shotgun sequence.
ACCESSION   DAMZFT010000238 DAMZFT010000000
VERSION     DAMZFT010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5051)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5051)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5051
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.238"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(<1..241)
                     /locus_tag="PN977_005006"
     CDS             complement(<1..241)
                     /locus_tag="PN977_005006"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225241.1"
                     /note="catalyzes the formation of prolyl-tRNA(Pro) from
                     proline and tRNA(Pro); Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HDI6044922.1"
                     /translation="MRTSQYLLSTLKETPADAEVISHQLMLRAGMIRKLASGLYTWLP
                     TGVRVLKKVENIVREEMNNAGAIEVLMPVVQPSELW"
     gene            complement(352..1059)
                     /gene="tsaA"
                     /locus_tag="PN977_005007"
     CDS             complement(352..1059)
                     /gene="tsaA"
                     /locus_tag="PN977_005007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HDI6044923.1"
                     /translation="MSSFQFEQIGVIRSPYKEKFAVPRQPGLVKSANGELHLIAPYNQ
                     ADAVRGLEAFSHLWILFVFHQTMEGGWRPTVRPPRLGGNTRMGVFATRSTFRPNPIGM
                     SLVELKEVVCHKDSVILKLGSLDLVDGTPVVDIKPYLPFAESLPDASASYAQSAPAAE
                     MAVSFTAEVEKQLLTLEKRYPQLTLFIREVLAQDPRPAYRKGEETGKTYAVWLHDFNV
                     RWRVTDAGFEVFALEPR"
     gene            complement(1056..1460)
                     /gene="rcsF"
                     /locus_tag="PN977_005008"
     CDS             complement(1056..1460)
                     /gene="rcsF"
                     /locus_tag="PN977_005008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rcs stress response system protein RcsF"
                     /protein_id="HDI6044924.1"
                     /translation="MRALPICLVALMLSGCSMLSRSPVEPVQSTAPQPKAEPAKPKAP
                     RATPVRIYTNAEELVGKPFRDLGEVSGDSCQASNQDSPPSIPTARKRMQINASKMKAN
                     AVLLHSCEVTSGTPGCYRQAVCIGSALNITAK"
     gene            complement(1578..2393)
                     /gene="metQ"
                     /locus_tag="PN977_005009"
     CDS             complement(1578..2393)
                     /gene="metQ"
                     /locus_tag="PN977_005009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter substrate-binding
                     lipoprotein MetQ"
                     /protein_id="HDI6044925.1"
                     /translation="MAFKFKTFAAVGALIGSLALVGCGQDEKDPNHIKVGVIVGAEQQ
                     VAEVAQKVAKDKYGLDVELVTFNDYVLPNEALSKGDIDANAFQHKPYLDQQLKDRGYK
                     LVAVGNTFVYPIAGYSKKIKSLDELQDGSQVAVPNDPTNLGRSLLLLQKVGLIKLKDG
                     VGLLPTVLDVVENPKNLKIVELEAPQLPRSLDDAQIALAVINTTYASQIGLTPAKDGI
                     FVEDKESPYVNLIVTREDNKDAENVKKFVQAYQSDEVYEAANKVFNGGAVKGW"
     gene            complement(2433..3086)
                     /gene="metI"
                     /locus_tag="PN977_005010"
     CDS             complement(2433..3086)
                     /gene="metI"
                     /locus_tag="PN977_005010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease MetI"
                     /protein_id="HDI6044926.1"
                     /translation="MSEPMMWLLVRGVWETLAMTFVSGFFGFVIGLPVGVLLYVTRPG
                     QIIANAKLYRTVSAIVNIFRSIPFIILLVWMIPFTRVIVGTSIGLQAAIVPLTVGAAP
                     FIARMVENALLEIPTGLIEASRAMGATPMQIVRKVLLPEALPGLVNAATITLITLVGY
                     SAMGGAVGAGGLGQIGYQYGYIGYNATVMNTVLVLLVILVYLIQFAGDRIVRAVTRK"
     gene            complement(3079..4110)
                     /gene="metN"
                     /locus_tag="PN977_005011"
     CDS             complement(3079..4110)
                     /gene="metN"
                     /locus_tag="PN977_005011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein
                     MetN"
                     /protein_id="HDI6044927.1"
                     /translation="MIKLSNITKVFHQGTRTIQALNNVSLHVPAGQIYGVIGASGAGK
                     STLIRCVNLLERPTEGSVLVDGQELTTLSESELTKARRQIGMIFQHFNLLSSRTVFGN
                     VALPLELDNTPKDEIKRRVTELLSLVGLGDKHDSYPSNLSGGQKQRVAIARALASNPK
                     VLLCDEATSALDPATTRSILELLKDINRRLGLTILLITHEMDVVKRICDCVAVISNGE
                     LIEQDTVSEVFSHPKTPLAQKFIQSTLHLDIPEDYQERLQAEPFADCVPMLRLEFTGQ
                     SVDAPLLSETARRFNVNNNIISAQMDYAGGVKFGIMLTEMHGTQQDTQAAIAWLQEHH
                     VKVEVLGYV"
     gene            4298..4870
                     /gene="gmhB"
                     /locus_tag="PN977_005012"
     CDS             4298..4870
                     /gene="gmhB"
                     /locus_tag="PN977_005012"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HDI6044928.1"
                     /translation="MAKSVPAIFLDRDGTINVDHGYVHEIDNFEFIDGVIDAMRELKK
                     MGFALVVVTNQSGIARGKFTEAQFETLTEWMDWSLADRDVDLDGIYYCPHHPQGSVEE
                     FRQVCDCRKPHPGMLLSARDYLHIDMAASYMVGDKLEDMQAAAAANVGTKVLVRTGKP
                     VTPEAENAADWVLNSLADLPQAIKKQQKPA"
BASE COUNT         1266 a         1281 c         1291 g         1213 t
ORIGIN      
        1 gccacagttc agatggctga accaccggca ttaacacctc gatcgcaccg gcgttgttca
       61 tctcttcacg cacgatgttt tcgacttttt tcagaacgcg cacgccggtc ggcagccagg
      121 tatataaccc ggaggccagc ttgcggatca tcccggcgcg cagcatcagc tgatggctga
      181 tcacctcggc gtcggcaggt gtctccttga gagtggagag caggtattgg ctagtacgca
      241 tgttgttacg gttccagttg gaaggtagaa caggctcaag gcgagcctgg gacaaaaaaa
      301 gtgatttagt ttaccagtgc aaaagaaatg tcaaaagaga agggcgtgaa tttaacgcgg
      361 ttccagcgca aagacttcaa aacctgcgtc ggtgacgcgc cagcgaacgt taaaatcatg
      421 cagccagacg gcataggttt tgcccgtttc ctcaccttta cgataggccg ggcgcgggtc
      481 ctgcgccagt acttcgcgga taaacagcgt taactgcgga taacgcttct ccagcgtcaa
      541 aagctgcttt tcgacctctg cggtaaaact caccgccatt tctgccgctg gcgcactttg
      601 cgcatagctg gcactggcat cgggaagcga ttcagcaaag gggagatacg gtttgatatc
      661 cactaccggc gtaccatcga cgagatccag actgccgagc ttcagaatca cgctatcttt
      721 atggcaaaca acctctttca gctctaccag cgacatacca atcgggttag ggcggaaagt
      781 ggagcgcgta gcgaaaacac ccattctggt attaccgcca agacgaggcg gacgcacagt
      841 tggacgccag ccgccttcca tcgtttgatg aaagacgaaa aggatccata aatggctgaa
      901 cgcttccagg ccgcgaacgg cgtcggcctg gttgtaggga gcaatgagat gcagttcgcc
      961 gttggcgctt tttaccagac ctggctggcg cggaacggcg aatttttctt tatagggcga
     1021 gcgaataacg cctatttgct cgaactggaa actgctcatt tcgccgtgat gttaagcgca
     1081 gaaccgatac atacagcctg acgatagcag cctggcgtac cgctggtgac ttcgcagcta
     1141 tgcagtaata ccgcattggc tttcattttc gaggcgttga tttgcatccg cttacgtgcg
     1201 gttggaatgc tcggcggaga gtcctgatta gaggcctggc aagagtcgcc actgacttca
     1261 ccgagatcgc ggaacggttt gccgactaat tcttctgcat tggtataaat tcggaccggc
     1321 gtggcgcgcg gcgctttcgg ttttgcaggc tccgctttcg gctggggtgc agtgctttga
     1381 acgggttcga caggggatct gcttaacatg gaacagccgc ttagcatgag tgctactaaa
     1441 cagatcggta aagcacgcat agtatttcct caatgtatga tcaaaacgtc aatattgaat
     1501 caggcgcttg taaaaatgac aagacgggca agcgcccgtc ctgaatgata ttacaaattg
     1561 tggaaacagc ctaaatatta ccagccttta acagcgccgc cgttaaacac tttgtttgct
     1621 gcttcgtaaa cttcgtcaga ctgataagcc tggacgaatt tcttcacgtt ttcggcgtct
     1681 ttgttgtctt cacgcgttac gattaggttt acgtacgggg actctttatc ttcgacaaag
     1741 ataccgtctt tcgctggagt caggccaatc tggctggcat aggtggtatt gataactgcc
     1801 agagcgattt gcgcgtcgtc cagagagcgc ggcagctgcg gtgcttccag ttcaacaatt
     1861 ttcagatttt ttgggttctc aacaacatca agaacggtcg gcagcaggcc aacgccatct
     1921 ttcagtttga tcaagcccac tttttgcagc agcagcagtg aacgaccaag gttagttggg
     1981 tcgtttggca cggcaacctg cgaaccatcc tgcagttcat ccagtgattt gattttcttg
     2041 gagtaacctg caatcggata aacaaaagtg ttgcctactg cgaccagttt gtagccacga
     2101 tctttcagtt gctgatcaag gtacggttta tgctggaagg cgttggcgtc gatatcgcct
     2161 ttgctcaatg cttcgtttgg cagaacatag tcgttgaagg tgaccagctc aacgtccagg
     2221 ccatatttgt ctttcgcaac tttctgcgcg acttctgcaa cctgctgttc ggcaccaaca
     2281 atcacgccga ctttaatgtg gtttggatct ttttcatcct gaccgcagcc taccagtgcc
     2341 agtgatccaa tcagggctcc cactgccgca aaggttttga atttgaacgc cataccttat
     2401 tccttcttca attatttatg ttgtgttgaa cgttacttgc gagtgacagc ccggacgatg
     2461 cggtcgcctg cgaactgaat taaataaacc agaatgacca gcaataccag taccgtattc
     2521 atcaccgtcg cgttatagcc gatgtagcca tactgatagc caatctgacc taaaccaccg
     2581 gcaccgactg caccacccat cgcggaataa ccgaccaggg taatcagggt gatagttgcc
     2641 gcattcacca gacccggcaa tgcttccggt aacagcactt tacgaacgat ctgcattggc
     2701 gtggcaccca ttgcgcggga ggcttcaatt aacccggttg ggatctccag cagagcgttc
     2761 tcgaccatac gggcaataaa cggtgctgca ccaacggtta acggaacaat cgctgcctgc
     2821 aaaccaatcg atgtaccgac aataacgcgg gtaaacggaa tcatccatac cagcaagata
     2881 atgaacggga tggaacggaa aatgttcaca atcgcagaaa cggtacgata cagcttcgcg
     2941 ttagcaataa tttgccccgg acgcgtgaca taaagcagga cgccaaccgg cagaccaatc
     3001 acaaacccaa aaaatccgga tacgaaggtc attgccagcg tttcccatac gccacgaacc
     3061 agcagccaca tcatcggctc agacataacc cagtacctct acttttacat ggtgttcctg
     3121 cagccaggca atggcggctt gcgtatcttg ttgtgtgccg tgcatttcag tcagcatgat
     3181 gccgaactta acgccaccgg cgtaatccat ctgcgcgcta ataatgttgt tgttgacgtt
     3241 gaaacgacgc gcggtttcag aaagcagtgg ggcatcgacc gattgaccgg taaactccag
     3301 acgcagcatc ggcacgcagt cagcgaatgg ctccgcttgc agacgttcct ggtaatcttc
     3361 cgggatatcc agatgcaggg tcgactgaat aaacttctgc gccagcggcg ttttcggatg
     3421 cgagaacact tcacttaccg tgtcctgctc gatcagttcg ccattgctga tcaccgccac
     3481 gcaatcacaa atgcgcttca caacgtccat ttcgtgagtg atcaacagaa tcgtcaaacc
     3541 cagacggcgg ttgatgtctt tcagcagttc gagaatagaa cgtgtcgttg ccgggtccag
     3601 cgcgctggtg gcttcatcac acagcaatac tttaggattg ctggctaacg cacgggcaat
     3661 cgccacacgc tgtttctgcc caccagaaag gttcgacgga tagctatcat gcttatcacc
     3721 aagaccaacc aatgacagca attccgtcac gcgacgtttg atttcgtctt tcggcgtgtt
     3781 gtccagctcc agcggcagag ccacgttgcc aaaaacagta cgcgaagaga gcaggttaaa
     3841 atgctggaaa atcataccaa tctggcggcg agctttagtc aactcagatt ctgacagcgt
     3901 ggtcagctcc tggccatcaa ccagcacgct accctcggtt gggcgctcca gcaggtttac
     3961 acaacgtata agtgtactct tacccgcgcc tgaggcaccg ataacgccat aaatttgtcc
     4021 agctggcaca tgcaggctga cgttgtttaa cgcctggatg gtgcgggtgc cctggtggaa
     4081 cactttggtg atattcgaaa gttttatcat tgattattta ttatcgtcat taagttagtc
     4141 gtggcatttc gaatgcctgg aacgggcaac gccgtcaatg aaatggatgt taaggcatcc
     4201 agacgtctaa atcaatcagg tttatgcgaa gagcactttc ttgcaggtcg aaacatgcga
     4261 tactagcgtc acatgcctta ttaaggagct ataaaaggtg gcgaagagcg tacccgcaat
     4321 ttttcttgac cgtgatggca ccattaatgt cgatcacggc tatgtccatg agatcgacaa
     4381 ctttgaattt atcgacggtg ttattgacgc catgcgcgag ctaaaaaaaa tgggctttgc
     4441 gctggtggta gtaaccaacc agtctggcat tgctcgcggt aaatttactg aagcacagtt
     4501 tgaaacgctg accgagtgga tggactggtc gctggcggac cgcgatgtcg atctggatgg
     4561 tatctattat tgcccgcatc atccgcaggg tagtgttgaa gagtttcgcc aggtctgcga
     4621 ctgccgcaaa ccacatccgg ggatgctttt atcggcacgc gattatttgc atattgatat
     4681 ggccgcttct tatatggtgg gcgataaatt agaagatatg caggcagcgg ctgcggcgaa
     4741 cgtgggaaca aaagtgctgg tgcgtacggg taaacctgtt acgcctgaag cagaaaacgc
     4801 ggcggattgg gtgttaaata gcctggcaga cctgccgcaa gcgataaaaa agcagcaaaa
     4861 accggcgtaa tgattaaaag atgagcggtt gaaataaaaa tgcatttttc cgcttgtctt
     4921 cctgaaacga ctccctataa tgcgcctcca tcgacacggc ggatgtgaat cacttcacac
     4981 aaacagccgg ttcggttgaa gagaaaaatc ctgaaattca gggttgactc tgaaagagga
     5041 aagcgtaata t
//
LOCUS       DAMZFT010000239         4892 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.239, whole genome shotgun sequence.
ACCESSION   DAMZFT010000239 DAMZFT010000000
VERSION     DAMZFT010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4892)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4892)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4892
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.239"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(105..224)
                     /locus_tag="PN977_005013"
     CDS             complement(105..224)
                     /locus_tag="PN977_005013"
                     /inference="COORDINATES: protein motif:HMM:NF022029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ash family protein"
                     /protein_id="HDI6044929.1"
                     /translation="MVVLAGLPKGRPVPFCAGSLNPVSATAIEIETSRGSSLN"
     gene            complement(447..1931)
                     /locus_tag="PN977_005014"
     CDS             complement(447..1931)
                     /locus_tag="PN977_005014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000124153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HDI6044930.1"
                     /translation="MARSCVTDPRWRELVALYRYDWIAAADVLFGKTPTWQQDLIIES
                     VQEQGSKTSVSSGHGTGKSDMTSIMIMLFIIMYPGARAIIVANKIQQVMTGIFKYIKI
                     NWATATSRFPWLADYFVLTETAFYEVTGKGVWTVVPKGFRLGSEEALAGEHADHLLYI
                     IDEASGVSDRAFGIITGALTGQDNRILLLSQPTRPSGYFYDTHHKLAKRPGNPDGVYT
                     AITLNSEESPLVTPAFIKMKLAEYGGRDNPMYMIKVRGLFPKSQDGFLLGRDEVERAT
                     RRKVKIAKGWGWLACVDVAGGTGRDKSVINIMMVSGQRNKRRVINYRMLEYTDVTETQ
                     LAAKIFAECNPERFPNITIAIDGDGLGKATADLMYEYYGITVQRIRWGKKMHSREDKS
                     LYFDKRAYANVQAAEAVKSGRMRLDKGNETIEEASKIPVGINSAGQWKVMSKEDMKKK
                     LNLHSPDHWDTYCFAMLADYVPQDEVLSVEDEAQVDEALAWLNE"
     gene            complement(1931..3124)
                     /locus_tag="PN977_005015"
     CDS             complement(1931..3124)
                     /locus_tag="PN977_005015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016246563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HDI6044931.1"
                     /translation="MTWDDHKKNFARLARDGGYTIAQYAAEFNLNPNTARRYLRAFKE
                     DTGTADSRKPNKPVRKPLKSMIIDHANDQRAGDHIVAEMAEKQRVNAVVSAAVENAKR
                     QNKRINDRSDDHDVITRAHRTLRDRLERDTLDDDGERFEFEAGDYLIDNVEARKAARA
                     MLRRSGADVLETTLLEKSLSHLLMLENARDTCIRLVQEMRDQQKDNDEGTPPEYRIAS
                     MLNSCSAQISSLINTIYSIRNNYRKESREAEKHALSMGQAGIVKLAYERKRENNWSVL
                     EAAEFIEAHGGKVPPLMLEQIKADLRAPKTNTDDEERQTAVGGPSLEDLDKVARERAA
                     NRRADAALWIEQRREEIADIVDTGGYGDVDTEGVSNDPWLEQDLDEDEEEDEEVTRKL
                     YGDDD"
     gene            complement(3211..3663)
                     /locus_tag="PN977_005016"
     CDS             complement(3211..3663)
                     /locus_tag="PN977_005016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001448129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Late promoter-activating protein"
                     /protein_id="HDI6044932.1"
                     /translation="MLLNWQGRHFMEINHSRITSYEIADYMIRTKSLLSAKELAAILE
                     KEYPHLDVDKRDVYLRLKAIAVSKYSSVLIDDSTRPRRFQIHSLNPEFFRRSRAPRRF
                     DEKLQNELYMTQDEKERREHQPWVMARQLFNKVVRQHRHYGNATSARI"
     gene            complement(3752..4795)
                     /locus_tag="PN977_005017"
     CDS             complement(3752..4795)
                     /locus_tag="PN977_005017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000648830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HDI6044933.1"
                     /translation="MKAVITPFVQKELGVATFKVDQEVRKLVEAGRKFIMEPVPRELI
                     EHMDDGLVVSEQTMATNEALQPFFNSDELFRRIGGIDALVAWLRRKEGQCQAADRSWC
                     DNHIVHAERDNSAVLLCWHHDNHYRMRGFNELKETLHNNRVNWILDVARQEMGLSNSH
                     DLSIQELCWWAFMRNMMHLMPEEVCRISINKMKATPQDSGPLKEADIRPYDDRATAYV
                     QMMEERAAPMRAKVCPVDVDSDPGMAHFKIPKLQSLKLPEYMDFVASRPCCGCGAAGA
                     GAHITPYIVRHSRLCAHDIYAIPLCQSCQRDIERDRDNWEKTHGRLAMHQRLFFDYAL
                     GVGAITSHSSSVR"
BASE COUNT         1189 a         1291 c         1112 g         1300 t
ORIGIN      
        1 gcaataacga agtgatagtg acgtttttgg caggagtaaa agcgccagat aaattcagga
       61 tgagcagggg tagggatagt agccacaatg gcagcctcct tttgctaatt taaggagcta
      121 ccgcgtgagg tctcaatctc aatggcggta gcactgactg ggttgagact accggcgcaa
      181 aagggaaccg gcctgccttt cggcagccca gccagcacta ccattgatct ctgagctaaa
      241 cgctacgtat ggctgtgcga tggcatgaca caaaaaaaag acgcttttgg cgtctgtgtc
      301 gccttttgca ttatccgggg tctcaatccc ggcacccgtt ttaatgaggt gcctgataag
      361 cataaaccga aaatgcctca aggcgcaaga ggtcaggttc aatgtaacat cggcagttaa
      421 aaaacacaat ttattagagc aaatattcat tcattaagcc atgccagagc ttcatcaacc
      481 tgcgcttcgt cttcgacgct aagcacttca tcctggggaa cataatccgc cagcatagcg
      541 aaacaatatg tatcccaatg gtctggtgag tgcaggttga gttttttctt catatcctcc
      601 ttactcatca ccttccattg acctgcggag ttaatcccta cagggatttt cgacgcttcc
      661 tcaatagttt cattaccctt atccagtctc atacgaccag attttacggc ctctgcggct
      721 tgaacgttgg cataagcacg tttatcaaag tacaggctct tatcttcacg gctatgcatc
      781 tttttacccc agcgtatacg ctgtacggta ataccataat actcgtacat cagatccgcc
      841 gttgctttac ccagcccatc gccgtctatc gctatggtga tatttgggaa tcgctcagga
      901 ttacattctg cgaaaatttt ggcggcaagc tgcgtttctg taacgtctgt gtattccagc
      961 attcgatagt tgattacacg gcgtttattt ctctggccgg acaccatcat gatattgata
     1021 acggacttat cccgtcccgt accaccagca acgtccacac atgcaagcca gccccatcct
     1081 ttggcaatct tgactttccg ccgcgtcgca cgttcaacct catcacgtcc aagaaggaag
     1141 ccatcctgtg atttagggaa tagtccgcgt accttaatca tgtacatagg gttatcacgc
     1201 ccgccgtact ccgccagctt cattttgata aatgctggcg ttaccagcgg tgattcctca
     1261 ctgttaagcg tgatcgccgt ataaacgcca tcagggttac caggacgctt ggccagttta
     1321 tggtgagtat cgtagaaata gccgcttggg cgtgtaggct gtgacagtaa taagatgcgg
     1381 ttatcctgtc cggtaagagc accggtgatg ataccgaaag ctctatcact gacaccggag
     1441 gcttcatcga taatatacag aagatgatct gcgtgttcac cggcgagagc ttcttcactt
     1501 cccagacgaa agcccttcgg tactacagtc catacacctt taccagtaac ctcatagaaa
     1561 gcggtttctg tcagaacaaa ataatcagca agccatggaa aacggctggt ggcagtagcc
     1621 cagtttatct tgatgtactt gaatataccg gtcattacct gctgaatttt gttcgcaacg
     1681 ataatggcgc gggcaccggg atacatgatt atgaacaaca tgatcatgat agaagtcatg
     1741 tctgatttcc cggtaccgtg accagacgaa acagatgtct tgctaccctg ttcctgcaca
     1801 gactcaataa tcagatcctg ctgccaggta ggtgttttgc cgaacaaaac atcagcggca
     1861 gcaatccagt cataacgata tagcgccacc agctcgcgcc aacgtggatc cgttacgcaa
     1921 cttctggcca ttaatcatca tccccgtata gcttgcgggt aacttcttcg tcttcctcct
     1981 cgtcttcgtc caggtcttgt tccagccatg ggtcgtttga tacaccttca gtatcaacat
     2041 ctccataacc gcctgtatca acgatatcgg cgatttcttc cctacgctgc tcaatccaca
     2101 atgcggcatc ggcgcggcgg ttggcggccc gttctcgcgc aactttgtcc agatcttcaa
     2161 gagaagggcc accgacggct gtttgccttt cctcatcatc ggtattggtc ttaggagcac
     2221 gcagatcggc tttgatttgc tccagcatca ggggcggcac ctttccgcca tgcgcctcga
     2281 tgaattcagc tgcttccagc actgaccagt tattttcacg ctttcgttcg tatgccagct
     2341 taacaatgcc agcttgcccc atagataaag cgtgcttttc cgcctcccgg ctttcttttc
     2401 gatagttatt ccggatgctg taaatggtgt tgatcaggct gcttatctgc gcggaacagc
     2461 tgtttagcat gctcgcgata cgatattcag gcggagtccc ttcatcattg tctttttgct
     2521 gatcgcgcat ttcctgcacc agacgaatac acgtatccct ggcgttctcc agcataagga
     2581 gatgagaaag agacttttcc agaagagtgg tttccagaac atcggccccg gaccgacgca
     2641 acatagcgcg cgcggccttc cgcgcttcaa cgttatctat caggtaatcg ccagcttcga
     2701 attcaaagcg ttcaccatca tcatccaggg tgtcgcgttc caggcgatca cgtaaggtcc
     2761 ggtgggcgcg ggtgatcacg tcatgatcat cagaacgatc atttatgcgc ttattctggc
     2821 gcttcgcgtt ctcgactgcg gcactgacaa cagcattaac tctttgtttt tcagccattt
     2881 cagccacaat gtgatcacct gcacgttgat cattagcgtg atcaatgatc atgcttttta
     2941 gtggttttct gacaggctta tttggcttac ggctgtccgc tgttccggtg tcttctttga
     3001 atgcacggag ataacgacgt gcggtgtttg ggttgagatt aaactcggcg gcatattgtg
     3061 cgatggtgta accaccatct cgcgccaggc gagcaaaatt cttcttgtga tcgtcccagg
     3121 tcacttatgc ttcctttcgt ataaaactct ttttgacgcg agggtaacga aagtcacatg
     3181 tcaaaaggcc cggaacgggc aagcaatcaa tcagatacgt gcggatgtgg cattaccgta
     3241 atgacggtgc tgacggacca ctttattgaa aagttgacgc gccatcaccc aaggctggtg
     3301 ctcccggcgt tccttttcgt cctgcgtcat atagagttcg ttctggagtt tttcatcaaa
     3361 ccggcgcgga gcgcggctgc ggcgaaagaa ttcaggattc agagagtgga tctgaaatct
     3421 acgtgggcgt gtactgtcat caatcaaaac agacgaatac ttagacacag cgatagcctt
     3481 taagcgcaga taaacatcgc gcttatcgac atccagatgc gggtattcct tttcaagaat
     3541 tgctgcgagt tctttcgctg atagaagaga tttagtgcgg atcatgtaat ccgcaatctc
     3601 gtacgatgtt attcgtgagt gatttatttc catgaagtgg cgtccctgcc agttaagtaa
     3661 catcctgtca cctactgatt agcccatgtc aactaatcaa cgtcgaatat aataccctcg
     3721 attaaagaaa tagcaataca ttagagcaat tttatctaac gctcgacgag tgacttgtga
     3781 tagcgccgac tccaagcgcg taatcaaaga acaatcgttg atgcatcgcc agcctaccgt
     3841 gcgtcttctc ccaattatcg cggtcacgct caatatcacg ctggcatgac tggcacagag
     3901 gaatagcata aatgtcatgc gcgcataatc gactatgacg aacgatataa ggcgtaatgt
     3961 gagcgccagc tcccgcagct ccacacccac agcatggacg ggaagcaaca aagtccatgt
     4021 actcaggtaa ttttagcgat tgcagttttg gtattttgaa atgcgccatg ccagggtcgg
     4081 agtcaacatc cacagggcat acttttgcac gcatcggcgc ggcgcgttct tccatcatct
     4141 gaacatatgc tgtagcgcga tcgtcatacg ggcgaatatc cgcctctttc agaggtccgc
     4201 tatcctgcgg agtagccttc atcttattta ttgatatgcg gcagacttct tccggcatca
     4261 ggtgcatcat gttgcgcatg aaagcccacc agcacagctc ctgaatactt aaatcatggc
     4321 tatttgaaag gcccatttcc tgacgggcga catccagtat ccagttaacg cgattattat
     4381 gcagcgtttc tttcagctca ttaaaaccac gcatccggta atggttatcg tgatgccagc
     4441 acagcaacac cgcgctattg tctcgttcag cgtggacaat atggttgtca caccaactac
     4501 gatctgcggc ctggcattga ccctctttcc tacgcaacca cgccaccagc gcgtcaattc
     4561 caccaatacg gcgaaacagt tcatcgctgt taaaaaacgg ctgcaacgcc tcatttgttg
     4621 ccatggtttg ctcggaaaca acgaggccgt cgtccatgtg ctcgattaac tcacgcggca
     4681 ccggctccat aataaattta cggccagcct ccaccagctt tctgacttcc tgatccactt
     4741 tgaatgtggc gacgccaagc tctttttgta caaagggagt aattacggct ttcacatcac
     4801 acctttcatc actgattggg ctttatctgc tgcccggcat tctctgttta agcacaacca
     4861 tttcctgacg gcataacaca gcaatagcgg tc
//
LOCUS       DAMZFT010000240         4745 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.240, whole genome shotgun sequence.
ACCESSION   DAMZFT010000240 DAMZFT010000000
VERSION     DAMZFT010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4745)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4745)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4745
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.240"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..1215
                     /locus_tag="PN977_005018"
     CDS             <1..1215
                     /locus_tag="PN977_005018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HDI6044934.1"
                     /translation="GPFPLAQLKKLNLLPSMASIQTTLVNNEVSKPLFDMAKGETPFE
                     INSRIGYSGDSSSDISLKPLNYEQKDEKVAFSGGEFQLNADRDGKAISLSGEAQSGRI
                     DAVNEYNQKVQLTFNNLKTDGSSTLASFGERVGNQKLSLEKMTISVEGKELALLEGME
                     ISGKSDLVNDGKTINSQLDYSLNSLKVQNQDLGSGKLTLKVGQIDGEAWHQFSQQYNA
                     QTQALLAQPEIANNPELYQEKVTEAFFSALPLMLKGDPVITIAPLSWKNSQGESALNL
                     SLFLKDPATTKEAPQTLTQEVDRSVKSLDAKLTIPVDMATELMTQVAKLEGYQEDQAK
                     KLAKQQVEGASAMGQMFRLTTLQDNTITTSLQYANGQITLNGQKMPLEDFVGMFAMPT
                     LNVPAVPAIPQQ"
     gene            complement(1260..2525)
                     /gene="uidC"
                     /locus_tag="PN977_005019"
     CDS             complement(1260..2525)
                     /gene="uidC"
                     /locus_tag="PN977_005019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide uptake porin UidC"
                     /protein_id="HDI6044935.1"
                     /translation="MRKIVAMAVICLTAASGLTSAYAAQLADDEAGLRIRLKNELRRA
                     DKPSAGAGRDIYAWVQGGLLDFNSGYYSNIIGVEGGAYYVYKLGARADMSTRWYLDGD
                     KSFGFALGAVKIKPSESSLLKLGRFGTDYSYGSLPYRIPLMAGSSQRTLPTVSEGALG
                     YWALTPNIDLWGMWRSRVFLWTDSTTGIRDEGVYNSQTGKYDKHRARSFLAASWHDDT
                     SRYSLGASVQKDVSNQLQSILEKSIPLDPNYTLKGELLGFYAQLEGLSRNTSQPNETA
                     LVSGQLTWNAPWGSVFGSGGYLRHAMNGAVVDTDIGYPFSLSLDRNREGMQSWQLGVN
                     YRLTPQFTLTFAPIVTRGYESSKRDVRIEGAGILGGMNYRVSEGPLQGMNFFLAADKG
                     REKRDGSTLGDRLNYWDVKMSIQYDFMLK"
     gene            complement(2564..3937)
                     /gene="uidB"
                     /locus_tag="PN977_005020"
     CDS             complement(2564..3937)
                     /gene="uidB"
                     /locus_tag="PN977_005020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide transporter"
                     /protein_id="HDI6044936.1"
                     /translation="MNQQLSWRTIVGYSLGDVANNFAFAMGALFLLSYYTDVAGVGAA
                     AAGTMLLLVRVFDAFADVFAGRVVDSVNTRWGKFRPFLLFGTAPLMIFSVLVFWVPTD
                     WSHSSKVVYAYLTYMGLGLCYSLVNIPYGSLATAMTQQPQSRARLGAARGIAASLTFV
                     CLAFLIGPSIKNSSPEEMVSVYHFWTIVLAIAGMVLYFICFKSTRENVVRIVAQPSLK
                     ISLQTLKRNRPLFMLCIGALCVLISTFAVSASSLFYVRYVLNDTGLFTVLVLVQNLVG
                     TVASAPLVPGMVARIGKKNTFLIGALLGTCGYLLFFWVSVWSLPVALVALAIASIGQG
                     VTMTVMWALEADTVEYGEYLTGVRIEGLTYSLFSFTRKCGQAIGGSIPAFILGLSGYI
                     ANQAQTTEVIMGIRTSIALVPCGFMLLAFVIIWFYPLTDKKFKEIVVEIDNRKKMQQQ
                     LISDIIN"
     gene            complement(3934..>4745)
                     /locus_tag="PN977_005021"
     CDS             complement(3934..>4745)
                     /locus_tag="PN977_005021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HDI6044937.1"
                     /translation="EEMLDWADEHGIVVIDETAAVGFNLSLGIGFEAGNKPKELYSED
                     AVNGETQQAHLQAIEELIARDKNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLD
                     PTRPITCVNVMFCDAHTDTISDLFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQE
                     KLHQPIIITEYGVDTLAGLHSMYTDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFA
                     DFATSQGILRVGGNKKGIFTRDRKPKSAAFLLQKRWTGMNFGEKPQQGGKQ"
BASE COUNT         1271 a         1262 c         1109 g         1103 t
ORIGIN      
        1 ggtcccttcc cccttgccca gctaaaaaaa ctgaacctgc tcccgtcgat ggcatcaatt
       61 caaaccacgc tggttaataa cgaagttagc aagccactgt ttgatatggc aaaaggtgaa
      121 acgccttttg agattaactc ccgcattggt tacagcggag attccagttc cgatatttcg
      181 ctcaagccgc tgaattacga gcaaaaggat gaaaaagtcg cctttagcgg cggcgagttc
      241 cagttaaatg cggacagaga cggcaaagct atctcccttt ccggagaggc gcaaagtggt
      301 cggatagacg cagttaacga atacaaccag aaagtgcagt tgacctttaa taatctgaaa
      361 accgacggtt ccagcacgct ggcaagtttt ggtgagcgcg taggaaacca aaaactgtca
      421 ctggaaaaaa tgaccatttc agtggaaggc aaagaactgg cactgctgga aggcatggag
      481 atcagcggta aatcggatct ggtcaatgac ggtaaaacga tcaatagcca actggattac
      541 tcgctaaaca gcctgaaggt acagaatcag gatctgggca gtggcaagct gactttaaaa
      601 gtcggccaga ttgatggtga agcctggcat cagtttagcc agcaatataa cgcgcaaact
      661 caggcgctgc tggcacagcc agaaattgcc aacaatcccg aactttatca ggagaaagtg
      721 acggaagcct tctttagcgc cctgccgctg atgttgaaag gcgatccggt gattactatc
      781 gcgccgctaa gctggaaaaa cagtcagggt gaaagtgcgc tgaatctgtc gctgttcctg
      841 aaagatccgg caacgactaa agaagcgccg caaacgctga cgcaggaagt agatcgttcg
      901 gttaaatctc tggatgcgaa actgaccatt ccggtggata tggcaactga gttgatgact
      961 caggtagcga agctggaagg ttatcaggaa gatcaagcga aaaaactggc gaaacagcaa
     1021 gttgaaggtg catcagcaat ggggcagatg ttccgtctga ccaccttgca ggacaatacc
     1081 atcaccacca gcctgcaata tgctaacggt cagataacgt taaacgggca gaaaatgcca
     1141 ctggaagatt tcgtgggtat gtttgcgatg ccgacattaa acgttccggc tgtacccgct
     1201 attccgcagc agtaattcac agccaaaacg ccggagattt ttctctccgg cgttttttct
     1261 tacttcagca taaagtcata ctgaatactc attttcacat cccagtaatt caggcgatcg
     1321 cccagcgtac tgccatcgcg cttttcccgc cctttatcgg cagcaagaaa gaaattcatc
     1381 ccttgtaacg gcccttcgct gacccgatag ttcatacccc ctaagatacc cgcgccttca
     1441 atccgcacat ctcgtttact ggattcatag ccgcgggtca caatcggtgc aaatgtcagc
     1501 gtaaattgcg gagttaaacg atagttgacg cccaattgcc aggactgcat tccttcacgg
     1561 ttacgatcaa gacttaatga aaagggatag ccaatgtcgg tgtccaccac ggcaccattc
     1621 attgcatggc gcaaataacc accactgcca aatacgcttc cccacggcgc attccaggtc
     1681 aattgtccac taaccaacgc cgtttcattg ggctggctgg tattacgact taaaccttcg
     1741 agttgcgcgt aaaagccgag caactcccct ttcaacgtat aattcgggtc gagcggtatg
     1801 cttttctcga gaatactttg taactgattg gaaacatctt tctgtaccga tgcccccaga
     1861 gaatagcgac tggtatcatc atgccaactg gcggctaaaa aagaacgtgc gcgatgttta
     1921 tcgtattttc ccgtctggct gttatacacc ccttcatcac gaataccggt tgttgaatcg
     1981 gtccataaaa atactcgtga acgccacatt ccccacagat caatatttgg cgttaaagcc
     2041 caataaccta atgctccttc agaaactgtc ggtaatgtac gttgcgaact gccagccatt
     2101 aacggaatac gataaggtaa gctaccataa ctataatccg tcccgaagcg acctaattta
     2161 agcagactac tttcactggg ttttattttt actgccccca gggcaaagcc aaaactttta
     2221 tcaccatcaa gataccaccg ggtactcata tcagcacgag cacctaattt ataaacataa
     2281 tacgccccgc cttcaacgcc aataatattg gaatagtaac cactattgaa atcgagcaat
     2341 cctccctgta cccatgcgta aatatctctt cccgcgccag cactgggctt atcagccctg
     2401 cgtaattcat ttttcagtct gatgcgtagt cccgcttcat cgtcagccag ttgcgccgca
     2461 taagcagagg taaggccaga ggcagccgtc aggcaaataa cggccatggc cactattttt
     2521 ctcatagttg cacctttgat gttctgatta tttttattga atattaatta atgatatcgc
     2581 tgattaattg ctgctgcatt tttttacgat tatcaatttc aaccacgatt tctttgaatt
     2641 ttttatccgt gagcggataa aaccagataa tgacgaatgc cagcagcata aatccgcaag
     2701 gtactaaggc aattgatgtg cggatgccca taataacttc cgtcgtttgc gcctgattgg
     2761 cgatatatcc gcttaatccc aaaataaagg caggaattga acctccgatt gcctgaccgc
     2821 atttacgggt aaatgagaat agtgaatagg tgagcccttc aattcgcacg ccggtcaggt
     2881 attcaccgta ttctacggta tcagcttcca gtgcccacat cacggtcatg gtaacgccct
     2941 ggccaattga tgcgatggcc aacgcaacca acgccaccgg cagtgaccag acggaaaccc
     3001 agaagaacag cagataaccg caggttccca gcaaagcgcc aatcaggaag gtattctttt
     3061 taccgatcct cgcgaccatc ccaggcacca gcggtgccga tgccacagta ccgaccaggt
     3121 tttgcaccag taccagcaca gtgaacagcc cggtatcatt taacacatag cgcacgtaga
     3181 acaacgacga ggcgctgacc gcgaaggtcg aaatcagcac acacagcgca ccgatgcaca
     3241 acataaacag cgggcgattc cgtttcaggg tttgcagact gatcttcaat gacggctgcg
     3301 ccacgatacg taccacattc tcacgcgtcg atttgaagca gatgaagtaa agcaccattc
     3361 cggcaatcgc cagcacaatc gtccagaaat ggtataccga caccatctct tccgggctgg
     3421 agttcttaat gctcggtcct atcagaaatg ccaggcagac aaaggtcaat gacgcggcaa
     3481 tcccgcgagc cgcgcccaga cgggcgcggg attgtggttg ttgggtcatc gcggtagcaa
     3541 gtgaaccata aggaatattc accaggctgt agcaaagccc gagtcccatg taggtcaaat
     3601 atgcatacac aactttgctg ctatggctcc agtcggtcgg tacccagaat accagcacgc
     3661 tgaagatcat taacggcgca gtaccgaaga gtaaaaacgg gcggaatttt ccccaacggg
     3721 tattcacact gtccaccact cgtccggcaa agacatcggc gaaggcatcg aatacccgca
     3781 ccagtaacag catggtgccc gccgcagcgg caccgacgcc agcgacgtcg gtgtagtaac
     3841 tcaacaggaa gagcgccccc attgcgaagg cgaagttatt ggcgacgtca ccgaggctgt
     3901 agccgacgat ggtgcgccag gagagttgtt gattcattgt ttgcctccct gctgcggttt
     3961 ttcaccgaag ttcatgccag tccagcgttt ttgcagcaga aaagccgccg acttcggttt
     4021 gcggtcgcgg gtgaagatcc ctttcttgtt accgccaacg cgcaatatgc cttgcgaggt
     4081 cgcaaaatcg gcaaaattcc atacctgttc accgacgacg gcgctgacgc gatcaaagac
     4141 gcggtgatac atatccagcc atgcacactg atactcttca ctccacatgt cggtgtacat
     4201 tgagtgcagc ccggctaacg tatccacgcc gtattcggtg atgataatcg gctgatgcag
     4261 tttctcctgc caggccagca gttctttttc cagaaccttc tctgccgttt ccaaatcgcc
     4321 gctttggaca taccaaccgt aataacggtt caggcacagc acatcaaaga gatcgctgat
     4381 ggtatcggtg tgagcgtcgc agaacattac attgacacag gtgatcggac gcgtcgggtc
     4441 gagtttacgc gttgcttccg ccagtggcgc gaaatattcc cgtgcacctt gcggacgggt
     4501 atccggttcg ttggcaatac tccacatcac cacgcttggg tggtttttgt cacgcgcaat
     4561 cagctcttca atcgcctgta agtgcgcctg ctgggtttcc ccgttgactg cgtcttcgct
     4621 gtacagttct ttcggcttgt tgcccgcttc gaaaccaatg cctaaagaga ggttaaagcc
     4681 gacagctgca gtttcatcaa tcaccacgat gccatgttca tctgcccagt cgagcatctc
     4741 ttcag
//
LOCUS       DAMZFT010000241         4733 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.241, whole genome shotgun sequence.
ACCESSION   DAMZFT010000241 DAMZFT010000000
VERSION     DAMZFT010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4733)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4733)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4733
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.241"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            207..791
                     /gene="pth"
                     /locus_tag="PN977_005022"
     CDS             207..791
                     /gene="pth"
                     /locus_tag="PN977_005022"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HDI6044938.1"
                     /translation="MTIKLIVGLANPGAEYAATRHNAGAWFVDLLAERLRAPLREEAK
                     FFGYTSRVTLGGEDVRLLVPTTFMNLSGKAVAAMASFFRINPDEILVAHDELDLPPGV
                     AKFKLGGGHGGHNGLKDIISKLGNNPNFHRLRIGIGHPGDKNKVVGFVLGKPPVSEQK
                     LIDEAIDEAARCTEMWFTDGLTKATNRLHAFKAQ"
     gene            908..1999
                     /gene="ychF"
                     /locus_tag="PN977_005023"
     CDS             908..1999
                     /gene="ychF"
                     /locus_tag="PN977_005023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HDI6044939.1"
                     /translation="MGFKCGIVGLPNVGKSTLFNALTKAGIEAANFPFCTIEPNTGVV
                     PMPDPRLDQLAEIVKPQRTLPTTMEFVDIAGLVKGASKGEGLGNQFLTNIRETEAIGH
                     VVRCFENDNIIHVSGKVNPADDIEVINTELALADLDTCERAIHRVQKKAKGGDKDAKA
                     ELAVLEKCLPQLENAGMLRALDLSAEEKAAIRYLSFLTLKPTMYIANVNEDGFENNPY
                     LDQVREIAAKEGSVVVPVCAAVEADIAELDDEERDEFMQELGLEEPGLNRVIRAGYKL
                     LNLQTYFTAGVKEVRAWTIPVGATAPQAAGKIHTDFEKGFIRAQTISFEDFITYKGEQ
                     GAKEAGKMRAEGKDYIVKDGDVMNFLFNV"
     gene            2191..2367
                     /locus_tag="PN977_005024"
     CDS             2191..2367
                     /locus_tag="PN977_005024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016158417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6044940.1"
                     /translation="MKKTFTFSLLAIVFASLLSACVPHHHHRHSDGPRGPAPSDKAMP
                     PSNGGPNHGSHGGY"
     gene            complement(2404..4323)
                     /gene="dhaR"
                     /locus_tag="PN977_005025"
     CDS             complement(2404..4323)
                     /gene="dhaR"
                     /locus_tag="PN977_005025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001467850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase operon
                     transcriptional regulator DhaR"
                     /protein_id="HDI6044941.1"
                     /translation="MSGAFNNDGRDISPLIATSWERCIKLMKRETWNVPHQAQGVTFA
                     SIYRRKKAMLTLGQAALEDAWEYMAPRECALLILDETACILSRNGDPQTLQQLSALGF
                     NDGTYCAEGIIGTCALSLAAISGQAVKTMADQHFKQALWNWAFCATPLFDSKGRLTGT
                     IALACPVEQTTAADLPLTLAIAREVGNLLLTDSLLAETNRHLNQLNALLESMDDGVIS
                     WDEQGNLQFINAQAARVLRLDATASQGRAITELLTLPAVLQQAIKQAHPLKHVEATFE
                     SQHQFIDAVITLKPIIETQGTSFILLLHPVEQMRQLMTSQLGKVSHTFAHMPQDDPQT
                     RRLIHFGRQAARSSLPVLLCGEEGVGKALLSQAIHNESERAAGPYIAVNCELYGDAAL
                     AEEFIGGDRTDNENGRLSRLELAHGGTLFLEKIEYLAVELQSALLQVIKQGVITRLDA
                     RRLIPIDVKVIATTTADLAMLVEQNRFSRQLYYALHAFEITIPPLRMRRGSIPALVNN
                     KLRSLEKRFSTRLKIDDDALARLVSCAWPGNDFELYSVIENLALSSDNGRIRVSDLPE
                     HLFTEQATDDVSATRLSTSLSFAEVEKEAIINAAQVTGGRIQEMSALLGIGRTTLWRK
                     MKQHGIDAGQFKRRG"
     gene            4551..>4733
                     /locus_tag="PN977_005026"
     CDS             4551..>4733
                     /locus_tag="PN977_005026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415718.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HDI6044942.1"
                     /translation="MKKLINDVQDVLDEQLAGLAKAHPSLTLHQDPVYVTRADAPVAG
                     KVALLSGGGSGHEPMHC"
BASE COUNT         1215 a         1203 c         1138 g         1177 t
ORIGIN      
        1 accctgtggg ttatgtgcgg gcgtgatcac aattacaacc cttatttcaa caaaacttta
       61 caaataaacg cctgaacacc ttggctttct aatagtccac tcaggcaatt attgcgttat
      121 ctgcatttga tacgcagttt tttgtcaagc ggggccgcaa ccagtaaact acgcgccagt
      181 tatgtacaca ctcaggacaa aaaaacgtga cgattaaatt gattgtcggc ctggcgaacc
      241 ccggcgctga atacgccgca acgcgacata atgctggtgc ctggttcgtt gacttactgg
      301 cagagcgatt gcgcgctccg ctgcgcgaag aggctaaatt ctttggttat acttcgcgag
      361 tcacccttgg aggcgaagat gtccgcctgt tagtcccgac tacatttatg aatctcagcg
      421 gcaaagccgt tgcggcgatg gccagttttt tccgtattaa tccggacgaa attctggtgg
      481 cccacgacga actggatctg cctcctggcg tcgccaaatt taagttgggc ggtggccatg
      541 gtggtcacaa tggactgaaa gacatcatca gtaaattggg taataaccct aactttcacc
      601 gtttacgcat cggaattggt catccaggcg ataaaaataa agttgtcggt tttgtgttag
      661 gcaaaccgcc tgttagtgaa cagaagttaa ttgatgaagc catcgacgaa gcggcacgtt
      721 gtactgaaat gtggtttaca gatggcttga ccaaagcaac gaaccgattg cacgccttta
      781 aagcgcaata agtcgttgtc tgcggcattt ttgccgagtg ccgtgtataa taggcaaagt
      841 tatttccatt tctgcaatct gttagcaata acaggttgat tattaagata ttaaggtgat
      901 ttaaatcatg ggattcaaat gcggtatcgt cggtttgccc aacgtcggga aatctaccct
      961 gttcaacgcg ctgaccaaag ccggtattga agcggccaac tttccattct gtaccattga
     1021 gccgaacaca ggcgtcgtac caatgcctga ccctcgcctg gatcaactgg ctgaaatcgt
     1081 aaaaccgcag cgtacgcttc ccacgaccat ggaatttgtc gatatcgccg gtctggtaaa
     1141 aggcgcatcg aaaggcgaag gtctgggtaa ccagttcctg accaacatcc gtgaaaccga
     1201 agcgatcggt cacgttgttc gctgctttga aaatgacaac atcattcacg tttccggcaa
     1261 agttaacccg gcggatgata ttgaagttat caacaccgaa ctggcgctgg cggatctcga
     1321 cacctgtgaa cgtgcgattc atcgcgtaca gaagaaagcc aaaggtggcg ataaagacgc
     1381 gaaagctgag ctggcggtcc tggaaaaatg cctgccccag ctggaaaacg caggtatgct
     1441 gcgcgcgctg gatttaagcg ctgaagagaa agcggctatt cgttacctga gcttcctgac
     1501 gctgaaacca acaatgtaca tcgccaacgt caacgaagac ggttttgaaa acaacccgta
     1561 tcttgaccag gtgcgtgaaa tcgcggcgaa agaaggttct gttgtggttc cggtttgtgc
     1621 tgctgttgaa gcagacattg ccgaactgga cgacgaagaa cgtgacgagt ttatgcagga
     1681 gcttgggctg gaagagccgg gcctgaaccg tgtgatccgt gccggttata agctgctgaa
     1741 cctgcaaact tacttcaccg ctggggtgaa agaagtacgt gcatggacca ttccggttgg
     1801 tgcaaccgcg ccgcaggccg ctggcaaaat ccatactgat tttgaaaaag gctttatccg
     1861 tgcacaaacc atctcgtttg aagatttcat cacttacaaa ggtgaacaag gcgcgaaaga
     1921 agcaggcaaa atgcgtgcag aaggcaaaga ttacatcgtt aaagatggcg atgtaatgaa
     1981 cttcctgttc aacgtctaat tccgctaata ctactctggc ggttaataac gagccattat
     2041 gtgagttgca cgtaatggct tttttacgcc tgctacgcgt cttctttcaa ttctgagtta
     2101 ccaaaactca taatcagact cctgacaata ttaacaatgc agcttttatg tgataacaat
     2161 cacagtgcat taaccgttca agagtttgtc atgaaaaaaa catttacctt cagcctgtta
     2221 gcaattgtct ttgcaagcct gttgagtgct tgtgttcccc accatcatca ccgccacagc
     2281 gatggccctc gtggtcctgc gccctccgat aaggcaatgc caccttcaaa tggtggacca
     2341 aatcacggca gtcatggtgg ctattaatta gcaattcata gcgggaaagt acatactctc
     2401 ccgctatcca cggcgcttaa actgccctgc atcaatgcca tgttgcttca ttttccgcca
     2461 cagcgtagtg cggccgattc caagtaaagc cgacatttcc tgaatgcgac cgcctgtgac
     2521 ctgggctgcg ttaataattg cctctttttc aacttccgca aatgacagac tggtggaaag
     2581 gcgagtggcg ctgacatcat ctgtcgcctg ctcggtaaac agatgttcag gtaaatcact
     2641 gacgcgaatg cgcccgttat cactactcag agcaagattc tcgatgacgc tgtaaagttc
     2701 aaaatcgttg cctggccatg cacaagaaac caggcgagcg agggcatcgt catcaatttt
     2761 cagccgcgta gagaagcgtt tttcaaggct gcgtaatttg ttattcacca gcgccggaat
     2821 gctgccacgc cgcatacgca gtggcgggat ggtaatttca aatgcatgca gcgcgtaata
     2881 cagctggcga ctaaaacgat tttgttccac cagcattgcg aggtccgcgg tcgttgtggc
     2941 aatcactttg acatcaatgg gtattaaacg ccgcgcatcc agtcgcgtga taaccccctg
     3001 cttgataacc tgaagcaaag cagactgtaa ctccaccgcc agatattcaa tcttttcaag
     3061 aaacagcgtg ccgccgtgcg ccagttccag ccgactcaga cgaccatttt cattgtccgt
     3121 gcgatcgccg ccaataaatt cttccgccag cgccgcatca ccatataact cacaattgac
     3181 ggcgatataa ggccccgctg cacgctcact ttcattatga attgcctgac ttagcagtgc
     3241 cttgcccacg ccctcttctc cacaaagcag gacaggaagg ctactgcgcg ccgcctggcg
     3301 accaaaatga atcaagcggc gggtttgcgg atcgtcctgt ggcatatgag cgaaagtatg
     3361 gctgactttt cctaattgac tggtcattaa ctgccgcatc tgttccacag gatggagcaa
     3421 caaaataaag ctggttccct gcgtttctat tatcggttta agggttatca ccgcatcaat
     3481 aaactggtgc tgactttcaa aggttgcttc tacgtgtttg agcggatgtg cctgttttat
     3541 tgcttgttgc aatacggcgg gtaacgttaa gagttcagtg atcgcccttc cctgacttgc
     3601 cgtcgcgtca aggcgcaaga cccgcgccgc ctgggcattg ataaattgca aattaccctg
     3661 ctcgtcccag ctaatcacgc catcatccat actttctaac agggcattaa gttgatttaa
     3721 atgacggtta gtttcagcga gcaaactgtc cgtcagcagt aaatttccga cctcgcgtgc
     3781 gattgccaac gtcaacggca aatcagctgc ggtagtttgt tcaaccggac acgccagcgc
     3841 tattgttccc gtcaatcggc ccttgctgtc aaacaacggc gttgcacaaa aggcccagtt
     3901 ccagagtgcc tgtttgaaat gttgatcggc catcgttttc acggcctgac cagagatagc
     3961 cgctaatgat agcgcacaag taccaataat tccctcggcg caatacgtgc cgtcattgaa
     4021 tcccagtgca cttagctgct gcaaggtttg cggatcgcca ttacggctga gaatgcaggc
     4081 ggtttcatcg aggataagca acgcacactc tcgcggtgcc atatattccc aggcatcttc
     4141 cagcgcagcc tgcccgagcg tcagcatcgc tttcttacgc cgataaatag aagcaaatgt
     4201 cacgccctgg gcctggtgtg gtacgttcca tgtctcccgt ttcatcagct taatgcatcg
     4261 ctcccaggag gttgcaatta agggagatat gtcccgacca tcgttgttaa aagcgccact
     4321 catatcccgc atcccttatg acaaaaggtg ttccggctcg caattttaag tattaataca
     4381 cgtaaaagtt tcaatatgga acattcatta aagaatatgt ttcactatga aacataattt
     4441 gggctaattt tcttttccct tgcctgatgc acaatggatt catcggcagt cagtggtcgc
     4501 cgtgtcgttg aacatcatcc atgccctacc gtaattgctg gagcaaaata atgaaaaaat
     4561 tgatcaatga tgtgcaagac gtactggacg aacaactggc aggactagcg aaagcgcatc
     4621 catcgctgac actgcaccag gatccggtgt atgtcacccg agctgatgcc cccgttgcag
     4681 gtaaagtcgc cctgctgtcg ggtggcggca gcggacacga gccgatgcac tgt
//
LOCUS       DAMZFT010000242         4717 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.242, whole genome shotgun sequence.
ACCESSION   DAMZFT010000242 DAMZFT010000000
VERSION     DAMZFT010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4717)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4717)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4717
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.242"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..1506
                     /locus_tag="PN977_005027"
     CDS             <1..1506
                     /locus_tag="PN977_005027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDI6044943.1"
                     /translation="ALSEIQIASAQKNLPFIAYIDKGFSAQGLVQLIRDSLPEQRKDE
                     AVGIILSNDPEHTRNLFDVMYGAKKPATPEKNFMVSVLCALCVDTGTGQPCNPGDTRQ
                     IISNLVDLAFREYGENNPRLYRAGTEPLVDLALEESGIAEQHDSGWWNAATWFEIRDM
                     LHMAGNIPAAQRAHYQAMPLLAEMSALLGQPSIRDVFGTVQRDNSAELLLDYIRRALD
                     QGHSDYPMMSGCTRFMLSPDTRVVAVDLNNVAGDKTPAGRLRTGIMYLLAGQIAGGDF
                     VLPQYQEEIRKNLDPRYHEVIFRRIEQLDQEVKTKVYDELHNAKGINFIWEALDTQEL
                     EQRKFGIRTVLSTQLLQDYPPAVLKSANTLWLLRYRPDEIPFLRDNFGVPEVTLRRFL
                     KMPEGAAPDGSGVPVLAVFRVKNGTLARILKFTLGPLELWALNSSPKDSALRRALTQE
                     LGSLRARQILAENFPRGSATSLIEHRARTHDSENVIHDLAAELIRKQGYHL"
     gene            1506..2123
                     /locus_tag="PN977_005028"
     CDS             1506..2123
                     /locus_tag="PN977_005028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000286860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraV"
                     /protein_id="HDI6044944.1"
                     /translation="MYSIHITPRCRRPARGQVFLKTLRVDGDSFSFTLPSACFTLRPS
                     PNPRVIFASDRRADGEMSGIRLALPGETGTLITTATWQVNVPERPPFDIQHVIHWLLP
                     SPLTALMSDNTWLWPDVPGTPALTAGDWPVMESDTPTLKRVKRRTFTLIDNRNRQGWI
                     TRRFQCFPLAPLPESLSHRVPDILYPPKTEKKECKDNLQPHCLHS"
     gene            2084..3289
                     /gene="traW"
                     /locus_tag="PN977_005029"
     CDS             2084..3289
                     /gene="traW"
                     /locus_tag="PN977_005029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001387490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraW"
                     /protein_id="HDI6044945.1"
                     /translation="MQRQSATTLLALLIGCAVIPATHAYSVTVVASQPVTTQIMPELA
                     AIQGVMAEMVRMQTATGTAINQSSEKLASVIAQDGQATRQQMIFSNETRRLEEARKSF
                     SVPDSICSESASGVAAESRRAAASAAARLSQGGGVSSKPIRERLSRAADSPTREAYDS
                     AGIHAGYCTEAEYARFGGTDVCPAVGELPGGDSQVRSLYQGAGTADTPAALTWDQKQI
                     DAATAYMKNTARPSAGRAPGKGEVGTQTGRTYVGLQNEYNGIIDAASHPQLSLIADST
                     PNEATRGALTEALQSPSAAAYFDRTASSEARTRGHMSQREFEAFEAGRRYANTDWQQD
                     LQGMEGDNLLRELLRTTALLNWQMNDLKEQIRQGNVIAGQQLALAARQYYGQRLGELS
                     QAMSQGSVR"
     gene            3286..3855
                     /gene="traX"
                     /locus_tag="PN977_005030"
     CDS             3286..3855
                     /gene="traX"
                     /locus_tag="PN977_005030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000650756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraX"
                     /protein_id="HDI6044946.1"
                     /translation="MKDDNSTDKKKPAPRLRRIGDIGINLFAPLAETRWMLRSCRHML
                     GQHKTRLKRLMPDRQPEIPVCMTWEEAVRASGLTVNELDRRLRRRRMFWRTGFWLMMI
                     PVLLLCGMALAATALPAITLIRILACLLLMSAIAALCASKTLIVSYRLWQLNERRVSE
                     DEQGTFPDYLQARHGWKQATWLAITHRQY"
     gene            3928..>4717
                     /locus_tag="PN977_005031"
     CDS             3928..>4717
                     /locus_tag="PN977_005031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000691807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DotA/TraY family protein"
                     /protein_id="HDI6044947.1"
                     /translation="MKIIPRALSAGLALAAVPAQAFVTYQDIVSAATNPDDLSRQALV
                     TIFGDVVTNPLTASSNTIIGNLFMVFNAIIAIMGIVWFLFIGIRHVVRTGHQGSVFNN
                     GRDVVGMLSIVSGFMMIVPTASGWSLAQLVMLWGASIMGIGSANIMVQTAAENIAEGY
                     SMTVQPVHVSTRTAARGILEMALCKHAMNAGLDDFNRTARSSTPPMSESSRTENGRYT
                     VTVSNGSATCGSVSLTVKGNGSNNQSALDYFFNPFIETEYQGVLT"
BASE COUNT         1073 a         1419 c         1304 g          921 t
ORIGIN      
        1 gcactgtcgg agatccagat agcctcagcc cagaagaacc tgcctttcat tgcctatatc
       61 gacaagggat tcagtgcaca gggactggta cagcttatcc gtgacagcct gccggagcag
      121 agaaaggacg aagccgtggg gatcattctg agcaatgatc cggaacacac ccgtaacctg
      181 tttgatgtga tgtacggtgc taaaaaaccc gccaccccgg aaaagaactt catggtatct
      241 gtactctgcg cactgtgcgt ggataccggc accggtcagc cctgtaatcc cggagacacc
      301 cggcaaatca taagcaacct ggtggatctg gccttcaggg agtatggcga aaacaacccc
      361 agactgtacc gtgcaggaac agagcccctt gtggatctcg ccctggaaga gagtggcatc
      421 gcagaacaac atgactctgg atggtggaat gctgccacct ggtttgaaat cagggacatg
      481 ctgcatatgg ctggcaatat ccccgcagcc cagcgggcac actaccaggc catgcccctg
      541 ctggctgaaa tgtccgccct gctgggacag ccatccatcc gggatgtgtt cggcacagtt
      601 cagcgcgata actcagcaga attgttactg gactacatcc gccgtgccct cgatcaggga
      661 cacagtgact acccgatgat gtccggctgt acccggttta tgctcagccc cgacacccgc
      721 gttgtggccg ttgatctgaa caatgtggcc ggtgacaaga cacctgccgg gcgtctgcgc
      781 accggcatca tgtacctgct ggcaggacaa atcgccggcg gggattttgt cctgccgcag
      841 tatcaggagg aaatacggaa aaatctcgac ccacgctacc atgaggtgat tttccgccgc
      901 attgaacagc ttgatcagga ggtcaagacc aaagtctatg acgaactgca caatgcaaaa
      961 gggatcaact tcatctggga agccctggac acccaggagc tggagcagcg taagttcggg
     1021 atacgcacag tgctgagcac ccagctactg caggactatc caccggcagt tctcaaatcc
     1081 gccaacaccc tctggttgct gcgttaccgc cctgacgaaa ttccgtttct gcgggataat
     1141 tttggtgtcc ctgaagtcac cctgcggcgc tttctgaaaa tgccggaagg ggctgcaccg
     1201 gatggcagcg gtgtcccggt actggctgtg ttccgtgtaa aaaacggtac gctggcccgg
     1261 atcctgaaat tcactctcgg tccactggag ctgtgggctc tgaattcctc acccaaagac
     1321 agcgccctga gacgtgcgct gacgcaggag ctcgggtcac tgcgggcacg ccagatcctg
     1381 gcagaaaact ttccccgtgg ttcagccacc tcactgatag aacaccgcgc ccgtacacat
     1441 gacagtgaaa acgtcatcca tgatctggct gcggaactca tccgtaagca gggatatcac
     1501 ctctgatgta cagcatacac atcactcccc gctgccggcg tcctgcacgg ggacaggtct
     1561 tcctgaaaac gctgcgcgtg gacggtgaca gtttcagctt caccctgccg tccgcctgct
     1621 tcacactgcg accgtccccc aatccccggg tcatttttgc cagtgacaga cgggctgacg
     1681 gggagatgag tggtattcgc ctggccctgc ccggagagac aggcacgctg ataacaacgg
     1741 ccacctggca ggttaacgtg ccggagcgac cgccttttga tatacagcat gtcattcact
     1801 ggcttctgcc ctcgcctctc acggcgctga tgtctgacaa cacctggctg tggccggatg
     1861 tccccggcac accggcactc acagccggag actggccggt catggaaagc gacaccccga
     1921 cactgaaacg cgtgaagcgt cgtaccttca cgctcatcga taaccgtaac cgtcagggat
     1981 ggatcacgcg ccgcttccag tgtttcccgc tggctccgct gccggaatca ctcagtcatc
     2041 gcgtaccaga catcctgtat cccccgaaaa cggagaaaaa agaatgcaaa gacaatctgc
     2101 aaccacactg cttgcactcc tgataggctg tgcggttatt cctgccaccc atgcctattc
     2161 tgtgaccgta gtggccagcc agcctgtcac cacacaaatc atgccggagc tggccgccat
     2221 tcagggcgtg atggcagaga tggtccgtat gcagacagcc accggcacag ccatcaacca
     2281 gagttcagag aagctggcct ctgtcatcgc acaggacggg caggccaccc gtcagcagat
     2341 gatattcagt aatgaaaccc gccggcttga ggaggccagg aaaagcttca gcgtgccgga
     2401 ctccatctgc agtgaatccg cctccggagt tgcagctgaa agtcgccgtg cagcagcgtc
     2461 tgcggctgca cgtctgagtc agggaggagg cgtcagcagt aaacccatcc gtgaacgtct
     2521 ctcccgggca gcagactcac caacccgcga ggcatatgac agtgcaggca ttcatgccgg
     2581 ttactgcacg gaagctgaat atgcccgctt tggcggaacc gatgtctgcc ctgccgtggg
     2641 cgagctgccg ggcggagaca gccaggtgcg atcgctctat cagggtgcag gcacagcaga
     2701 tacccctgcc gccctcacct gggatcagaa acagattgat gctgccacgg cttacatgaa
     2761 aaacaccgcc agaccgtcag cgggtcgtgc cccggggaaa ggtgaagtgg gaacacagac
     2821 cgggcgcacg tatgtgggcc tgcagaatga atacaacggc atcatcgatg ccgcctctca
     2881 tccgcaactg tcactgatag ctgacagtac ccctaatgag gccacccgtg gagcactgac
     2941 tgaagccctg cagtcaccat ccgctgcggc atattttgac cggaccgcat catcagaggc
     3001 ccgcacccgt ggtcacatga gccagcggga gtttgaggcc tttgaagccg gcagacgcta
     3061 tgcaaacacc gactggcagc aggatctgca gggcatggaa ggcgataacc ttctccgtga
     3121 actgctgcgc actaccgccc tgctgaactg gcagatgaac gacctgaaag agcagatacg
     3181 ccagggtaat gttatcgccg ggcagcagct ggcactggcg gcacgacagt attacggaca
     3241 gcgtctcggt gaactgtcac aggccatgag ccaggggagc gtaagatgaa agacgacaac
     3301 agcaccgaca aaaaaaaacc ggccccccgt ctccgccgca tcggtgacat cgggataaac
     3361 ctgttcgcac cactggccga aacccggtgg atgctgcgca gctgccgcca tatgctgggc
     3421 cagcataaaa cgcgcctgaa gcgcctcatg cccgaccggc aaccggagat tccggtctgt
     3481 atgacctggg aagaagccgt cagggccagc ggccttacgg tcaacgaact ggatcggcgt
     3541 ctgcgacgtc gcaggatgtt ctggcggaca ggattctggc tgatgatgat accggtactt
     3601 ctgctgtgcg gcatggccct tgctgccaca gcgcttccgg ccatcaccct gatacgtata
     3661 ctggcctgcc tgttactgat gagtgccatt gcagcactgt gtgcctcaaa aaccctgatt
     3721 gtgtcatacc gcctctggca actgaatgaa cggcgggtca gcgaggacga acagggcacc
     3781 ttccccgatt acctgcaggc ccggcacggc tggaaacagg ccacatggct ggccattacc
     3841 caccggcagt actgaccgtc cggtctgcaa ttccttttcc tcattccgga ggctgatgcc
     3901 ctgcatccct tcggttccgg agttatcgtg aaaatcatac ccagagcact gagtgcgggt
     3961 ctggcgctgg ctgccgtacc tgcacaggca ttcgtcacct atcaggacat tgtgagcgcc
     4021 gccaccaatc cggatgacct gtcgcgccag gcactggtca ccatttttgg cgacgtggtc
     4081 acaaatcccc tgacagccag cagcaacacc attatcggca acctgttcat ggtgtttaac
     4141 gcaattatcg ccattatggg gatagtctgg ttcctgttta ttggtatccg ccatgttgtc
     4201 cgcaccggcc accagggcag tgtctttaac aacggacggg acgtcgtcgg aatgctcagt
     4261 attgtgagcg gttttatgat gattgtgccc accgcttccg gctggagtct ggcacaactg
     4321 gtcatgctct ggggcgcatc catcatgggc attggttccg ccaatatcat ggttcagact
     4381 gctgcagaaa acatcgctga aggctattca atgaccgtac agccagttca tgtctccacc
     4441 cgcacggcag cacggggaat actggagatg gcgctgtgca aacacgccat gaatgcaggc
     4501 ctcgatgact ttaaccgtac tgcccgttca tcaacgcccc ccatgagcga atcctcacgg
     4561 acagagaacg gcaggtatac cgtcaccgtt tcaaatggca gtgccacctg cgggagtgtg
     4621 agtctcaccg tgaaagggaa tggcagcaac aaccagtccg cgctggacta ttttttcaat
     4681 cccttcattg aaacggagta tcagggagtc ctgacgg
//
LOCUS       DAMZFT010000243         4707 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.243, whole genome shotgun sequence.
ACCESSION   DAMZFT010000243 DAMZFT010000000
VERSION     DAMZFT010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4707)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4707)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4707
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.243"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..296
                     /gene="hisE"
                     /locus_tag="PN977_005032"
     CDS             <1..296
                     /gene="hisE"
                     /locus_tag="PN977_005032"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoribosyl-ATP diphosphatase"
                     /protein_id="HDI6044948.1"
                     /translation="DTAHQWLFLYQLEQLLAERKSADPETSYTAKLYASGTKRIAQKV
                     GEEGVETALAATVHDRFELTNEASDLMYHLLVLLQDQGLDLTTVIENLRKRHQ"
     gene            complement(392..1372)
                     /gene="wzzB"
                     /locus_tag="PN977_005033"
     CDS             complement(392..1372)
                     /gene="wzzB"
                     /locus_tag="PN977_005033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416531.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen chain length determinant protein
                     WzzB"
                     /protein_id="HDI6044949.1"
                     /translation="MRVENNNVSGQNHDPEQIDLIDLLVQLWRGKMTIIISVIVAIAL
                     AIGYLAVAKEKWTSTAIITQPDVGQIAGYNNAMNVIYGQAAPKVSDLQETLIGRFSSA
                     FSALAETLDNQEEPEKLTIEPSVKNQQLPLTVSYVGQTAEGAQMKLAQYIQQVDDKVN
                     QELEKDLKDNIALGRKNLQDSLRTQEVVAQEQKDLRIRQIQEALQYANQAQVTKPQIQ
                     QTGEDITQDTLFLLGSEALESMIKHEATRPLVFSPNYYQTRQNLLDIESLKVDDLDIH
                     AYRYVMKPTLPIRRDSPKKAITLILAVLLGGMVGAGIVLGRNALRNYNAK"
     gene            complement(1518..2684)
                     /gene="ugd"
                     /locus_tag="PN977_005034"
     CDS             complement(1518..2684)
                     /gene="ugd"
                     /locus_tag="PN977_005034"
                     /EC_number="1.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 6-dehydrogenase"
                     /protein_id="HDI6044950.1"
                     /translation="MKITISGTGYVGLSNGLLIAQNHEVVALDILPSRVAMLNDRISP
                     IVDKEIQQFLQSDKIHFNATLDKNEAYRDADYVIIATPTDYDPKTNYFNTSSVESVIK
                     DVVEINPYAVMVIKSTVPVGFTAAMHKKYRTENIIFSPEFLREGKALYDNLHPSRIVI
                     GERSERAERFAALLQEGAIKQNIPTLFTDSTEAEAIKLFANTYLAMRVAYFNELDSYA
                     ESLGLNTRQIIEGVCLDPRIGNHYNNPSFGYGGYCLPKDTKQLLANYQSVPNNLISAI
                     VDANRTRKDFIADAILSRKPQVVGIYRLIMKSGSDNFRASSIQGIMKRIKAKGVEVII
                     YEPVMKEDSFFNSRLERDLATFKQQADVIISNRMAEELKDVADKVYTRDLFGSD"
     gene            complement(2932..4338)
                     /gene="gndA"
                     /locus_tag="PN977_005035"
     CDS             complement(2932..4338)
                     /gene="gndA"
                     /locus_tag="PN977_005035"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HDI6044951.1"
                     /translation="MSKQQIGVIGMAVMGRNLALNIESRGYTVSIFNRSREKTEEVIA
                     ENPGKKLVPYYTVKEFVESLETPRRILLMVKAGAGTDAAIDSIKPYLDKGDIIIDGGN
                     TFFQDTIRRNRELSAEGFNFIGTGVSGGEEGALKGPSIMPGGQKEAYELVAPILTKIA
                     AVAEDGEPCVTYIGTDGAGHYVKMVHNGIEYGDMQLIAEAYSLLKGGLNLTNEELAQT
                     FTEWNNGELSSYLIDITKDIFTKKDEDGNYLVDVILDEAANKGTGKWTSQSALDLGEP
                     LSLITESVFARYISSLKDQRVAASKVLSGPQAQPAGDKDEFIEKVRRALYLGKIVSYA
                     QGFSQLRAASEEYNWDLDYGEIAKIFRAGCIIRAQFLQKITDAYAENPQIANLLLAPY
                     FKQIADDYQQALRDVVAYAVQNGIPVPTFAAAVAYYDSYRAAVLPANLIQAQRDYFGA
                     HTYKRIDKEGVFHTEWLD"
BASE COUNT         1289 a         1110 c         1029 g         1279 t
ORIGIN      
        1 gcgacaccgc tcaccagtgg ctgttcctgt atcaactgga acaactgctc gccgaacgta
       61 aatctgccga cccggaaacc tcctacactg ccaaactgta tgccagcggc accaaacgca
      121 ttgcgcagaa agtaggcgaa gaaggcgtgg aaaccgcgct ggccgcgacg gtacatgacc
      181 gctttgagct gaccaacgag gcatctgatt tgatgtatca cctgctggtg ttgttgcagg
      241 atcaggggct ggatttaacg acggtaattg agaacctgcg taaacggcat cagtgagttg
      301 cggggtaagc ggatgcgata ttgttgccgc atccggcaaa aaaacgggca aggtgtcacc
      361 accctgccct ttttctttaa aaccgaaaag attacttcgc gttgtaattg cgtagagcat
      421 tacgccccag cacaatcccc gcgccaacca tgccacccag cagcaccgcc agaatcaagg
      481 taattgcctt tttcgggcta tcacgacgaa taggtaacgt cggtttcatt acatagcggt
      541 aagcatgaat atcaagatca tcaaccttta agctttcgat atcaagcagg ttttgacgag
      601 tctgatagta gtttggtgag aacaccaacg gacgggtcgc ctcatgctta atcatcgact
      661 ccagcgcttc gctccccaaa aggaacaacg tatcttgtgt gatatcttcg ccagtctgtt
      721 gaatctgcgg ttttgtcacc tgcgcctgat tcgcatactg caacgcttcc tgaatctgac
      781 ggatacgcag atctttctgc tcctgcgcaa ccacttcctg cgttcttaaa gagtcctgca
      841 agtttttccg tcccagagca atgttgtcct tgagatcctt ttctaactct tgattcactt
      901 tatcgtcaac ttgctgaatg tattgggcca acttcatttg tgcgccctct gcagtttgcc
      961 caacataaga aacagtcaat ggtaattgct ggttcttaac agaaggttcg atggtaagtt
     1021 tttctggttc ttcctgatta tccagcgttt ctgctaatgc agagaaggca gaactgaagc
     1081 gaccaattaa cgtctcctgc aaatccgata ctttcggtgc agcctgacca tagataacat
     1141 tcatggcatt gttatagcca gcaatttgcc ccacatcggg ctgagtgata attgctgttg
     1201 acgtccattt ctccttcgct actgccaaat atccaatagc cagggcaata gccacaatga
     1261 cggaaatgat gattgtcatc ttgccacgcc acaactgcac tagtaaatca atcaaatcaa
     1321 tctgttccgg gtcatggttt tgcccagaaa cattattatt ttctactctc atcattaccc
     1381 taactgacgg aaatttccaa gtccgcatag tttagcgata atcctcatga atgctatagg
     1441 ataattgata aaaataatga attgttacgc aagtaacaaa agacaatcag ggcgtaaata
     1501 gccctgataa caggatgtta gtcgctgcca aagagatcgc gggtgtagac cttatctgcc
     1561 acatctttaa gctcttctgc catacggttg gaaataatga catcggcttg ttgtttgaaa
     1621 gtggcgagat cacgttccag gcgagagttg aagaatgaat cttctttcat caccggttcg
     1681 tagatgatca cttcaacgcc tttcgccttg atacgtttca taatcccctg aatggaagac
     1741 gcacggaagt tatctgaccc gctcttcata atcagacgat aaatacccac cacttgcggt
     1801 ttgcgtgaca aaatggcatc ggcaataaag tctttacgtg tgcggttagc atcgacaatt
     1861 gccgagatca ggttattcgg cacagactgg tagttcgcca gtaactgctt ggtatctttc
     1921 ggcagacaat aaccaccata gccaaacgac gggttgttgt aatggttgcc aatacgcgga
     1981 tcgagacaaa cgccttcgat aatctggcga gtattcagac ctaaactttc tgcatagcta
     2041 tccagttcgt taaagtacgc cacgcgcatc gccaggtagg tgtttgcaaa aagtttaatc
     2101 gcttctgctt cagtggagtc ggtaaacagg gtcgggatat tttgcttaat cgctccttcc
     2161 tgtaacagag cagcaaaacg ttctgcgcgt tctgaacgct caccgatgac aatacgtgaa
     2221 ggatggagat tatcgtaaag ggctttaccc tcacggagaa attccgggga gaatataata
     2281 ttttcagtgc gatatttctt atgcatcgct gcggtaaaac caacgggaac cgttgatttg
     2341 atcaccataa ccgcataagg atttatctca actacgtctt taattactga ttctacactg
     2401 gatgtattga aataattagt tttaggatca tagtcggttg gagtggcgat gatgacataa
     2461 tcagcatccc ggtaggcttc atttttatct aatgtggcat taaagtgtat tttatctgat
     2521 tgcaaaaact gctgaatttc cttatcaaca ataggagata tccgatcatt cagcatagca
     2581 acgcgtgacg gtaaaatatc taatgccaca acctcatgat tttgtgcgat tagaagcccg
     2641 tttgacaagc cgacatagcc agtaccggaa atggtgattt tcatttcatg ctctcagaat
     2701 taacttaact gtgaatcatg atgtttttag catcctgata agagttaaaa gttttaacgc
     2761 tacgttgtaa gatggtcaac gcaacgaaaa acaattcaga atttctttat aagaatagct
     2821 gatatttatt aagttaatat taagcagtga aatttagtct ataagataat tggtaaaaaa
     2881 aacccggcat aatatacgcc gggcctcaat tttattgttg attaaatcag attaatccag
     2941 ccattcggta tggaacacac cttctttatc aatgcgctta taagtatgcg caccgaaata
     3001 gtcacgctgt gcctggatca ggttcgcagg cagaacagcg gcgcggtagc tgtcataata
     3061 ggcaaccgca gcggcgaagg tcggaaccgg gataccgttc tgtaccgcgt aagcgacgac
     3121 atcgcgcagc gcctgctggt agtcatcggc gatttgcttg aagtacggag ccagcagcag
     3181 gttagcgatc tgcggatttt cggcataagc atcggtgatt ttctgcagga actgcgcacg
     3241 gatgatgcag ccagcacgga aaatcttcgc gatttcgccg tagtccagat cccagttgta
     3301 ctcttcagac gccgcacgca gttgagagaa gccctgagcg taagaaacga ttttgcccag
     3361 atacagtgca cggcgaactt tttcgatgaa ctcatcttta tcgcctgctg gctgcgcttg
     3421 cgggccagag agaactttag atgcggcaac acgctgatct ttcagagaag agatataacg
     3481 tgcaaacaca gactcggtaa tcagcgacag cggttcaccg agatccagcg cgctctggct
     3541 ggtccattta ccggtacctt tgttagccgc ttcatccaga atcacatcaa ccaggtagtt
     3601 accgtcttca tcttttttag tgaagatgtc tttagtgatg tcaatcaggt agctgctcag
     3661 ttcaccgtta ttccactcgg taaaggtctg cgccagttct tcgttagtga ggttcaggcc
     3721 acctttaagc agagaatagg cttcagcaat cagctgcata tcgccatatt caataccgtt
     3781 gtgaaccatc ttcacatagt gacctgcgcc atcggtacca atataggtaa cgcacggttc
     3841 gccatcttca gcaacggctg cgattttggt caggatcggc gcaaccagtt cataggcttc
     3901 tttctgccca ccaggcatga tggaaggccc tttcagcgcg ccctcttcac caccggaaac
     3961 accggtaccg atgaagttaa agccttcggc agaaagctca cggttacggc gaatggtgtc
     4021 ctggaagaat gtattaccac catcaatgat gatgtcacct ttatcgaggt acggcttgat
     4081 ggaatcaata gcagcatccg tgccagcacc tgctttcacc attaacagga tgcgacgagg
     4141 cgtttccaga gattcaacaa actctttcac cgtatagtaa ggaaccagtt tcttgcctgg
     4201 attttcggca atcacttctt ccgtcttttc acgggaacgg ttgaaaatag agacggtata
     4261 accacggctt tcgatgttga gcgcaaggtt gcgccccatc actgccatac cgattacgcc
     4321 gatctgttgc tttgacattg tttactcctg tcacgaaatt accgtcttag cggttcggtt
     4381 taaacgtaat gttaacatac aaagatacaa ctaagaaaaa ataagagtgc aatcttcaga
     4441 attattatat aattacttcc gaatatattg gtaaataatt tttaagacct ttagagggag
     4501 taatcgcgga atgcttctta ctaacaaaat gcagaatcca gatatatgtg actgcaaacc
     4561 aagtttaact tttagccgat aaagttggaa ttcacttttt atatacctca gcccttttcg
     4621 cctagccaac atattatcac caactctagc taatattaac gactcattaa tgttacacac
     4681 atcaaatcca gatgccacta ctcgtaa
//
LOCUS       DAMZFT010000244         4695 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.244, whole genome shotgun sequence.
ACCESSION   DAMZFT010000244 DAMZFT010000000
VERSION     DAMZFT010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4695)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4695)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4695
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.244"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(<1..173)
                     /locus_tag="PN977_005036"
     CDS             complement(<1..173)
                     /locus_tag="PN977_005036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDI6044952.1"
                     /translation="MEISTLQIIAIFLFSCIAGMGSVLDEFQTHRPLIACTVIGLILG
                     DLKTGIMLGGTLE"
     gene            complement(239..733)
                     /locus_tag="PN977_005037"
     CDS             complement(239..733)
                     /locus_tag="PN977_005037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HDI6044953.1"
                     /translation="MNITLARIDDRLIHGQVTTVWSKVANAQRIIICNDEVYNDEVRR
                     TLLRQAAPPGMKVNVVNIEKAVAVYHNPQYQDETVFYLFTRPQDALAMVRQGVKIGTL
                     NIGGMAWRPGKKQLTKAVSLDDDDINAFHELNNLGVILDLRVVASDPSINIIDKINEQ
                     LIAN"
     gene            complement(733..1140)
                     /locus_tag="PN977_005038"
     CDS             complement(733..1140)
                     /locus_tag="PN977_005038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sorbose transporter subunit IIA"
                     /protein_id="HDI6044954.1"
                     /translation="MVNAIFCAHGKLACAMLESVQMVYGDARVEAVEFVPGENAGDIV
                     AKLEKLVSIHNHDEWLIAVDLQCGSPWNAAAMLAMRNPRLRVISGLSLPLALELVDNQ
                     DSMNIDELCEHLTQIAKQICVVWRQVATAEEDF"
     gene            complement(1150..1956)
                     /locus_tag="PN977_005039"
     CDS             complement(1150..1956)
                     /locus_tag="PN977_005039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDI6044955.1"
                     /translation="MQTWLNLQDKIIIVTGGASGIGLAIVDELLAQGANVQMVDIHGG
                     DGQYENHKGYQFWPTDISSAKEVNHTVAEIIQRFGRIDGLVNNAGVNFPRLLVDEKAP
                     AGQYELNEAAFEKMVNINQKGVFLMSQAVARQMVKQHDGVIVNVSSESGLEGSEGQSC
                     YAATKAALNSFTRSWSKELGKHGIRVVGIAPGILEKTGLRTPEYEEALAWTRNITVEQ
                     LREGYTKNAIPIGRAGRLAEVADFVCYLLSERASYITGVTTNIAGGKTRG"
     gene            complement(2026..2973)
                     /locus_tag="PN977_005040"
     CDS             complement(2026..2973)
                     /locus_tag="PN977_005040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HDI6044956.1"
                     /translation="MENSDDIRLIVKIAQLYYEQDMTQAQIARELGIYRTTISRLLKR
                     GRDQGIVTIAINYDYNENLWLEQQLKQKFGLKDVVVVSGNDEDEETQLAMMGLHGAQL
                     LDRLLEPGDIVGFSWGRAVSALVENLPQAGQSRQLICVPIIGGPSGKLESRYHVNTLT
                     YSAAAKLKGESHLADFPALLDNPLIRNGIMQSQHFKTISAYWDNLDVALVGIGSPAIR
                     DGANWHAFYGGEESDDLNARQVAGDICSRFFDIHGAMVETNMSEKTLSIEMNKLKQAR
                     YSIGIAMSEEKYSGIVGALRGKYINCLVTNSSTAELLLK"
     gene            3321..4193
                     /gene="rluF"
                     /locus_tag="PN977_005041"
     CDS             3321..4193
                     /gene="rluF"
                     /locus_tag="PN977_005041"
                     /EC_number="5.4.99.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2604) synthase RluF"
                     /protein_id="HDI6044957.1"
                     /translation="MLPDSSVRLNKYISESGICSRREADRYIEQGNVFLNGKRATIGD
                     QVKPGDVVKVNGQLIEPREAEDLVLIALNKPVGIVSTTEDGERDNIVDFVNHSKRVFP
                     IGRLDKDSQGLIFLTNHGDLVNKILRAGNDHEKEYLVTVDKPITDEFIRGMGAGVPIL
                     GTVTKKCKVKKEAPFVFRITLVQGLNRQIRRMCEHFGYEVKKLERTRIMNVSLSGIPL
                     GEWRDLTDDELIDLFKLIENSSSEAKPKAKAKPKTVGIKRPVVKMEKTAEKGGRPASN
                     GKRFTSPGRKKKGR"
     gene            complement(4194..4466)
                     /locus_tag="PN977_005042"
     CDS             complement(4194..4466)
                     /locus_tag="PN977_005042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3811 domain-containing protein"
                     /protein_id="HDI6044958.1"
                     /translation="MALPRITQKEMTEREQRELKTLLDRARIAHGRVLTNSETNSIKK
                     EYIDKLMVEREAEAKKARQLKKKQAYKPDPEASFSWSANTSTRGRR"
BASE COUNT         1157 a         1197 c         1092 g         1249 t
ORIGIN      
        1 aattccagcg taccaccgag cataattccg gtttttaaat caccgagaat taaaccaatc
       61 accgtacagg caattaacgg acgatgagtc tgaaattcat ccagcacgct gcccattccg
      121 gcaatacagg aaaaaagaaa tatagcaata atttgtaggg tacttatttc catgatttgt
      181 caccttttaa gtgtcgtcat ccctgcctgt taaagcatat gtacacaggc gctattattt
      241 aatttgcgat taactgttcg tttattttgt cgataatatt gattgaggga tctgacgcta
      301 cgacgcgtaa atccagtatg acgccaagat tatttaactc atgaaatgcg ttaatatcat
      361 cgtcatctaa tgaaactgct ttggttaact gtttcttacc aggacgccag gccataccgc
      421 caatattcag ggtacctatt tttaccccct ggcgaaccat tgctaaggca tcttgtgggc
      481 gagtgaaaag ataaaaaacg gtttcgtcct gatattgcgg gttgtgataa accgcgacgg
      541 ctttttcaat attgaccacg ttaaccttca ttcccggtgg cgcagcctga cgcaataatg
      601 tccggcgaac ttcatcgtta taaacttcgt cattgcagat aataatgcgt tgggcgttag
      661 ccaccttaga ccagacggta gtgacctggc cgtggatcag gcggtcatca atgcgggcga
      721 gcgtaatgtt catcagaaat cctcctccgc tgttgccacc tgtctccaga cgacgcagat
      781 ttgctttgcg atttgcgtca ggtgttcaca cagttcatca atattcatgc tgtcctggtt
      841 atcaaccagt tcgagcgcca gcggcagaga aaggccgctg attacccgca gacgaggatt
      901 gcgcatcgca agcatcgctg ccgcattcca cgggctgccg cactgtaaat ccacggcgat
      961 tagccactcg tcatgattgt gaatgctcac taacttctcc agctttgcca caatgtctcc
     1021 ggcgttctcg ccgggcacaa attcaacggc ctcgaccctg gcatcgccgt acaccatctg
     1081 aaccgattcc agcatggcgc aggctaattt gccgtgggcg caaaaaatgg cattgaccat
     1141 aaagcctcct tagccgcgcg ttttgccgcc cgcaatgtta gtggttactc cggtgatata
     1201 gctggcgcgt tcagacagca gataacaaac aaaatcagcc acttctgcta atcttccggc
     1261 gcgcccaata ggaatggcgt ttttggtata gccttcacgc agctgctcga cggtgatatt
     1321 gcgcgtccac gccagcgctt cttcatattc cggcgtacgc agtcctgttt tttccagaat
     1381 ccccggcgcg ataccgacca cacggatacc gtgcttaccc agctctttcg accaggagcg
     1441 cgtgaagcta ttgagcgcgg ctttggtcgc ggcgtaacag ctttggcctt ctgagccttc
     1501 cagcccactt tctgaggaaa cattcacaat cacgccatca tgttgtttga ccatctgtcg
     1561 cgccaccgcc tgcgacatca gaaaaacgcc tttctggttg atattgacca ttttttcgaa
     1621 tgccgcttcg ttgagttcat actgcccggc aggcgctttc tcatcgacca gcagacgcgg
     1681 gaaattgacc ccggcgttat tgaccagacc gtcgatgcga ccaaaacgct ggataatttc
     1741 tgctaccgta tgatttacct ctttggcgct ggaaatatcg gtcggccaga actgataacc
     1801 tttatggttt tcatattgac catcgccacc gtgaatatcg accatctgta cattcgcgcc
     1861 ttgtgctaat aattcatcca caatcgccag accaataccg gatgcgccgc cggtgacaat
     1921 aatgatttta tcctgcaaat ttaaccacgt ttgcataaca gactcctgat tttagataat
     1981 agggacccgc cgtaattttt attacggtaa acatcgggaa gatttttatt tcagtaatag
     2041 ttcagctgtg ctgctattcg ttaccagaca attaatatat tttccacgca gtgcaccaac
     2101 aattccgctg tatttttctt cgctcatggc aatgccaatg gaataccgtg cctgctttaa
     2161 tttattcatt tcgatagaga gtgttttttc gctcatattc gtttcaacca ttgcgccgtg
     2221 aatatcaaaa aagcgcgagc aaatatcgcc agcaacctgg cgggcattca ggtcgtcact
     2281 ctcttcacca ccataaaacg catgccagtt agcgccgtcg cgaatggccg gtgagccaat
     2341 tcccaccagg gcgacatcca gattatccca gtaggcagag atggttttaa agtgctgaga
     2401 ctgcatgatc ccattacgaa ttaatgggtt atccagcaga gccggaaaat ccgcgagatg
     2461 cgattcccct ttcagcttcg ctgccgcgct gtaggttaat gtgtttacgt gatagcggct
     2521 ttcgagttta ccggacgggc cgccaataat cggcacgcaa attaactgcc gcgattgccc
     2581 cgcctgcggc aagttttcaa ccagtgcgct caccgcgcgg ccccaggaaa aaccgacaat
     2641 atcgccaggt tccagcaagc gatccagcag ttgcgcgccg tgtaacccca tcatcgccag
     2701 ttgagtctct tcatcctcat catttcccga caccaccaca acgtctttca ggccaaactt
     2761 ttgcttcagt tgctgctcca gccagagatt ttcgttgtag tcatagttga tggcgatggt
     2821 gacaattccc tgatcgcggc ctcgtttaag caagcggctg atggtggtgc ggtaaatacc
     2881 cagttcgcgc gcgatttgcg cctgcgtcat atcctgttcg taatagagtt gggcaatctt
     2941 cacaatcaaa cggatatcgt cactgttttc cattgccgcc tcctggttct gcacatttgt
     3001 gcaaatacgc tttctcaccg cttgatctga tataacccca tcatgatggc ctttcaaagc
     3061 gatttttcac tctttgcatg gggagaaata gcgctttctc ttgcacattt gtttctctgc
     3121 gtagtagtca tcagcacagg tgtgcagcga ttcgtaatat gttgattatc cttattttgc
     3181 caggcgaatt tgcatcgtac ttccggagac acttttgtga taaaaggcgt ggcagatcac
     3241 atttaatttc gacgttgccg cataaccctg tttttccccg ctaagaggta cggtatattc
     3301 cgcgttttac aggagaattt atgctgcccg actcatcagt ccgtttaaat aaatacatca
     3361 gcgaaagcgg aatttgctca cgccgcgaag cggatcgcta tatcgagcaa ggcaatgtgt
     3421 tccttaatgg caagcgagcc accattggcg atcaggtgaa acccggcgac gttgtgaaag
     3481 taaacggtca gttgattgag cctcgggaag ccgaagattt ggtacttatc gccctgaaca
     3541 agcccgtcgg tattgtcagc accaccgaag atggcgagcg cgataacatt gtcgatttcg
     3601 ttaaccacag caaacgcgtg ttcccgattg gccgcctgga taaagattcc caggggctga
     3661 ttttcctcac caatcacggc gatctggtga ataagatcct ccgtgcgggt aacgatcatg
     3721 agaaagagta tctggtgacg gttgataaac cgattaccga tgaatttatt cgtggtatgg
     3781 gggcgggagt accgatcctc gggacggtga cgaaaaagtg caaagtcaaa aaagaagcgc
     3841 cgtttgtctt ccgcattacc ctggtgcagg ggctgaaccg tcagatccgt cgcatgtgcg
     3901 agcactttgg ttacgaagtg aaaaagctgg aacgtacgcg cattatgaac gtcagtttaa
     3961 gcggcattcc gctgggtgag tggcgcgatt taaccgacga tgagttaatc gacctcttta
     4021 agctcatcga aaattcctct tccgaggcaa aacctaaagc gaaggccaag ccgaaaacgg
     4081 taggcatcaa acgtccagtc gtcaagatgg aaaaaacggc ggaaaaaggc ggtcgcccgg
     4141 cgtccaacgg taagcgtttt acctcgccgg ggcgaaaaaa gaagggacgt taatcagcgc
     4201 ctgccgcgcg tcgacgtatt tgctgaccag ctaaatgacg cctcaggatc cggtttataa
     4261 gcctgctttt tctttaactg gcgggctttt ttcgcttcag cctcacgctc gaccattaat
     4321 ttatcgatgt actctttctt aatgctgttg gtttcggagt tggtcagtac gcgaccatgc
     4381 gcaatccgcg cccgatccag cagtgtttta agttcacgct gctcgcgttc agtcatctct
     4441 ttttgggtaa tacgggggag tgccatgatg gtgccctcag tgagccagag agttcagtgt
     4501 acgggaattc gtttgcgagc agaactgata atattcattt tgccttctct taactattta
     4561 tctctttcga taattaatta gagatgcaga taaaaaaatt aaaggcaatt attctccgat
     4621 aaagacgttg gttaacagag tacaggctcg tttattgata agcatagtga caggaaaatc
     4681 aatacggccc gaaat
//
LOCUS       DAMZFT010000245         4571 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.245, whole genome shotgun sequence.
ACCESSION   DAMZFT010000245 DAMZFT010000000
VERSION     DAMZFT010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4571)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4571)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4571
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.245"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(<1..646)
                     /locus_tag="PN977_005043"
     CDS             complement(<1..646)
                     /locus_tag="PN977_005043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF945 domain-containing protein"
                     /protein_id="HDI6044959.1"
                     /translation="MRLASRFGYANQIRRDRPLTHEELMHHVPGIFGEDKHTSRSRNY
                     TYIPTITVLESLQREGFQPFFACQTRVRDPGRRGYTKHMLRLRRDGEINGQHVPEIIL
                     LNSHDGTSSYQMLPGYFRFVCQNGCVCGQSLGEVRVPHRGDVVDRVIEGAYEVVGVFD
                     RIEEKRDAMQSLILPPPARQALAQAALTYRYGDEHQPVTTADILTPRRREDYGQD"
     gene            complement(832..1716)
                     /locus_tag="PN977_005044"
     CDS             complement(832..1716)
                     /locus_tag="PN977_005044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001557377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosome-binding GTPase"
                     /protein_id="HDI6044960.1"
                     /translation="MSFSHSSLSAHVKSHLTFLPEEIRQKILEYLHSVIQYEPVIGIM
                     GKSGAGKSSLCNAIFQSRVCATHPLNGCTRQAHRLTFQPGERRMTLVDLPGTGETPQH
                     DQEYRALYRQLLPELDLIIWILRADERAYAADIAMHQFLLNEGADPSRFLFVLSHADR
                     IHPAEEWNSQSNTPSRQQELSLATVTARVATLFPSSFPVLPIAAPAGWNLPAFVSLMI
                     HALPPQATSAVYSHIRNEKRTAQDQQHAQQAFGDAIGQSFDAAIARFSFPAWMLQLLR
                     KARDRIIRLLVTLWDRLF"
     gene            1817..3040
                     /locus_tag="PN977_005045"
     CDS             1817..3040
                     /locus_tag="PN977_005045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001107223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6044961.1"
                     /translation="MAKNKGICRNKKCSNPVSEFDEANALRLCDDHYQLWKRNKENRR
                     VRNICRIISKYDKSLDREIFDRVIDAARNQTGKRIVEDAKILHQLRSSMQVRKSLHKG
                     VLRSREWLNTQELLSRKNISVTGDSFKSYIKDIVRLYCYKFLYSREYLNKNAYKKQYQ
                     RVINPREFNVPILQLEVAWINNNYRSSDSSSDIIILPRMIRRMVVFNDTKINKQLEEV
                     LGGVARKKVAVRNKGGLYTRIKKENDFLSMDRFMVEIENIIRPKIFPTSIYPPFSLSF
                     IERELPLLNLAHSQYKRAISKKEVNSIFYTLRKTHVALWLHLEMFALVAFIKLYTGKG
                     FEFIKNMCEFIDYDKEIIKFTHEKLLGEIRRNIQVNLKVNPDNFPELIEIYNSCFSKN
                     ILLYDVQDNGFKWCL"
BASE COUNT         1286 a          880 c         1092 g         1313 t
ORIGIN      
        1 ggtcctgacc gtaatcctcc cggcgtcgtg gtgtcagaat gtcggcggtg gtgacgggct
       61 gatgttcgtc accataacgg taagtcaggg cggcctgtgc cagcgcctga cgagccggtg
      121 gtggtaaaat cagtgactgc atggcatcac gcttttcctc tatccggtca aacacgccga
      181 ccacttcgta agccccttca atgaccctgt ccaccacgtc tccccggtgc gggacacgca
      241 cctctccgag agactgacca cagacacatc cgttctgaca cacgaaccgg aagtaacccg
      301 gcagcatctg gtagctggag gtgccgtcat gggagttgag caggatgatt tcagggacat
      361 gctgtccgtt tatctccccg tcacggcgca gacgcagcat gtgctttgtg tatccccggc
      421 gtcccgggtc acgcacgcgg gtctggcagg caaagaacgg ctgaaagcct tcccgttgca
      481 ggctttccag cacggtgatg gtggggatgt atgtatagtt ccggctccgg gaggtgtgct
      541 tatcctcccc gaaaatcccg ggcacatggt gcatcagctc ttcatgtgtc agcggacggt
      601 cacggcgtat ctggttcgca taaccaaaac gactggctaa tcgcatactt tgctccttat
      661 cagtggttaa gactgactga cgtaatcaat gaaaaagccg catctcccgg agaagacgct
      721 gcctgatgga tgaaatgaat gaggtttacc aaaagccctt cagcattgtg gtaaagccgc
      781 cggaaatgcg ttccctggtc atcgtctgtg ccggtcaaaa agcggggaaa ttcagaacag
      841 gcgatcccac agtgtgacca gcaggcggat aatgcgatca cgggctttac gcagaagctg
      901 cagcatccag gccggaaagc tgaaccgggc aatggccgcg tcaaagctct ggccgatggc
      961 gtcaccaaaa gcctgctgtg cgtgttgctg atcctgcgca gtgcgctttt cattccggat
     1021 atgcgaataa accgcgctgg ttgcctgtgg tggcagggcg tggatcatca gtgacacaaa
     1081 tgccggaaga ttccagcctg caggggcagc tatggggagt accggaaatg atgaagggaa
     1141 cagggttgcc acccgggctg ttactgttgc cagtgagagt tcctgctgac gggatggcgt
     1201 gttgctctgg ctgttccatt cttcagccgg gtggatgcga tcagcatggc tgagaacaaa
     1261 cagaaaacgc gagggatcgg caccttcatt cagtaaaaac tgatgcatgg cgatgtcggc
     1321 agcatacgca cgttcatcag cacgcaggat ccagataatc agatccagtt cgggcagtaa
     1381 ctgacggtaa agtgcccggt attcctgatc atgctgtggt gtttcgccag tgccgggcag
     1441 atcgacgagc gtcattctgc gttcaccagg ctgaaaggta agacgatgag cctgacgggt
     1501 gcagccgttc agtggatgcg tggcgcagac acgggactga aaaatggcat tgcacaggct
     1561 gctttttccg gcaccggatt tacccataat gccaatcacg ggttcgtact gaatgacgct
     1621 gtgaagatat tcaaggattt tctggcgtat ttcttcagga agaaaggtca gatgtgattt
     1681 tacgtgtgcg gaaagggaag aatggctgaa tgacataccg gtatcctgtt gatttcactg
     1741 aaccggatca cattaatccg gtctctcagt caggtaatat ggacgtgaaa aattttccat
     1801 tgacgggaag aagagtatgg ctaaaaacaa aggtatctgt cggaataaaa aatgcagcaa
     1861 tcctgtttca gaatttgatg aggcaaatgc cctgaggtta tgtgatgatc attatcagtt
     1921 atggaaacga aataaagaga accgtcgggt caggaatatt tgtcgtataa tcagtaaata
     1981 tgacaaaagt ctggaccggg agatatttga cagagtaata gatgctgcaa gaaatcaaac
     2041 gggaaagaga attgttgagg acgcaaaaat attacatcaa ttgcgcagtt caatgcaagt
     2101 aagaaaaagt cttcataagg gagtactccg gtcacgggaa tggctcaata cacaggagtt
     2161 actgagcaga aaaaatatat ctgttacagg tgactcattt aaatcttaca taaaggatat
     2221 tgtcagatta tattgctata agtttctcta cagcagagaa tatcttaata aaaacgcata
     2281 caaaaaacaa tatcagagag ttattaaccc cagagaattt aatgttccga tacttcaact
     2341 tgaagttgca tggatcaata ataattaccg ttcaagtgat tcaagctctg atattatcat
     2401 ccttcccagg atgataagaa gaatggttgt atttaatgat acgaagataa ataaacaatt
     2461 agaggaagtg ttgggggggg tagcacgaaa aaaagtcgca gtaagaaata aaggcggtct
     2521 ctataccaga ataaaaaaag aaaatgattt tctttctatg gatagattta tggtggaaat
     2581 agaaaatatc ataagaccta aaatatttcc gacatcaatt tacccgcctt tttcactttc
     2641 atttatcgag agagaattgc cactactgaa tctggcacac tcacagtaca aaagagcaat
     2701 aagtaaaaaa gaggtgaatt caatttttta tactttgcga aaaactcacg tggcattatg
     2761 gctgcatctt gaaatgtttg ctcttgtggc attcatcaaa ctgtatacag gaaaagggtt
     2821 tgaattcata aaaaacatgt gtgaatttat agattatgat aaagaaataa taaaattcac
     2881 gcatgaaaaa ctgctgggtg aaattaggcg taacattcag gtgaatttaa aggtgaatcc
     2941 tgataatttt cctgaattaa tagaaatata caattcatgc tttagtaaaa atatattgtt
     3001 gtatgatgtt caggataatg gttttaaatg gtgtttatga tgtaaaaata ttaaattcat
     3061 attacatttt aattatcgac aaaaaacaca taaagaaatc taaagggaag gggtgtcaaa
     3121 tgagctgata tagcgccagt ttggtatggt catgtgattt gatgattaaa gcttacggat
     3181 aataacactc ctctgtgagc catataaatg agcatgccat aatcgtcagt tcagagataa
     3241 aactgactga atgatcttat gttatggtta gcagtgttta ccggatacga tgacttatct
     3301 ctctcgcatc atatggattc actttcatgg ggtaaagcga tgggactaca gacgctgatt
     3361 atgatggttt gctgtaaagt tagcacaagt ttttgctgta cagaatacgg agtaagaaaa
     3421 ataattccag agtaaatctg ctctgctttt gaacagaaaa gattcatgct taccgtgaaa
     3481 aactgaaacc aacattcagt ctgcatggat gtcttaagat gttgagaatg tcggatatgg
     3541 tattatatac accttttcca ttaggtcctt ttatgttttc attaagtcgt tttgtatgta
     3601 ttccttcagt gattcattag tgcatgttta cgcatttaca cacttttccc attaatctcc
     3661 tttaaatgtg tttcattacg cattgctgta cacatttttg aactgataca catccaagtt
     3721 agtaaacctg tcagttctga ttttgcgtta actaaatcag tttaatgctg ctgtaaaaca
     3781 atccttcctg tttaagcaat caatctgcta ttcgatttta ctgatgtgac gtttaacctt
     3841 tcctgagctt tggttgtttg tggtttttgg tttgtgtttc gacgttaagc tgagaaaagt
     3901 atctttacgc taaatcaacc cgaactttcc tgaggtgatc tgacgttctt ttcgatgtgc
     3961 ttacgacgat tctgttaaac gactttctgg atgttttgat aacaatcaga ttgaggtatg
     4021 cattaacgat tcctgacgtt ttgctttttc atgtaaggtc attgttttcg tcatgatgtg
     4081 gtttttcttc ttgcattgcg aaagtgtgat gattggtttt ggttttttga ttcagatgag
     4141 atttacgtta tcgatggtta atcgtttagg tatgttgaaa agtaacatac tcaagataag
     4201 aaaatcgatt ctgatgggga tgatcttgtt acgacgttat ctgtaaacag gtcttaagcg
     4261 tgtcatttcg catccgcgat tgagcgatgt tgttaagctg caggtttgat ttgcattgtc
     4321 tggatgcgtt tctggtgttt tgttgattcg tttcccttat gactttgtcg tttttaaatc
     4381 cattcgctgt tgttgtgtgt ttttaccctg cgtgcaggtt tgtcttttcg ttcgtaggag
     4441 attcattcgc gattggcgtt gcacaatggt gggatgcggt aaagttttac cgcggtgatt
     4501 aatgatgtga agtcaatcca aatcaacgga gatctctcat catgaatcaa ccaatacaca
     4561 atgcttactg g
//
LOCUS       DAMZFT010000246         4564 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.246, whole genome shotgun sequence.
ACCESSION   DAMZFT010000246 DAMZFT010000000
VERSION     DAMZFT010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4564)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4564)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4564
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.246"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(230..616)
                     /locus_tag="PN977_005046"
     CDS             complement(230..616)
                     /locus_tag="PN977_005046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000968513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HDI6044962.1"
                     /translation="MMCLKYPKPEVMTEVMPGGSVFFLPPQGKPGVADLAQPHLQRLR
                     SQLERRLGTLHRVVCQPQRVGQSSSVAVTAEGACGEVHLLLTVGGHESWPSEEEYRHP
                     RWYIQVVDAADLFYLLLWLCDGGQEA"
     gene            complement(613..3318)
                     /locus_tag="PN977_005047"
     CDS             complement(613..3318)
                     /locus_tag="PN977_005047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006858949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative transfer ATPase"
                     /protein_id="HDI6044963.1"
                     /translation="MTRDAEAAVYHAGPSVIDFLPWVEYLDEEQCLLLDDGVSVGAVY
                     SVTPAATEGRTEERLAQIRDMVEDALQDSFDEYDTHPWVVQFYCQDENDVDAYLDYLK
                     GYVKPHAQGTAFTEAWLGEMARHLRGIARPEGLFTDTLVTGQPWRGQQRRTRMVVYRR
                     IGKNNNDPMSPVAMLNQVCDRVVSALGGAGIRCTRMNGQQVHSWLLRLFNPAPDWIAR
                     EELYRMASRVDPRERGDDVMPVMTDFAESLWFNPPYSDPENGVWWLDAMPHCAVVVEK
                     LRTPPEPGTITGEMPRGEKTVNALMDTFPEGTVMCMTIVVQPQDTLEDRFTRLSKNAV
                     GENTESIRARQDVKVVKELLGNRHKLYRAGLTFLLRAEDMDSLKRRRVELTTVLLGAG
                     LQPVRPEFDVGPLNSWLRALPMCFDPDTDKKQWYTRLMWVQHMAGLLPVTGRDTGTGH
                     PGFSFFNRGGDVLTFDPLNKLDRTQNAHLLLFGPTGAGKSATLCASLSQVMAVHRPRL
                     FIAEAGNSFGLLADYFESLGLSVNKISVRPGTGVTLPPFADAHKLVEEGQDLQCVDEH
                     SLPDIEDEAGDEGEDKRDILGEMEISARMMITGGDPKEEAALKRADRAMIREALLMAT
                     HRTYREGRQMLPEDLQSALWEISRDSSRNDVRNAKAAEMAESLGMFTQKGSFEAELFN
                     REGELWPEADVTLIDLGHLAREGYEAQMALTMVSMTNMINNIAERDQYLGRDIVFAVD
                     EAHIVTVNPLLSPYMTKVVKMWRKLGAWLWLATQNLKDYPDVSEKMLNMAEWWVCLTM
                     PPEEVNNIARFKSLTEEQKAVLMSASKLSGCYTEGVVLAKQIEALFRAVPPSLYLALG
                     MTEKEEKAERRMLMKEHQCSELEAAFHVARKLDRLRGLSSMEAEV"
     gene            complement(3462..3866)
                     /locus_tag="PN977_005048"
     CDS             complement(3462..3866)
                     /locus_tag="PN977_005048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000825933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03751 family conjugal transfer lipoprotein"
                     /protein_id="HDI6044964.1"
                     /translation="MKRLLFVMSVALVLSGCATSKEELLPAGEQTMLELWQGKTAGGG
                     QRHALAARETLRRPLTVTEQQGMNAEAYSYSRTQESEISQQFPRLPNPDMVMYVFPHL
                     AGGTSPVPGYSTVFPFYSRTQYALPGERTEAL"
     gene            complement(3884..>4564)
                     /locus_tag="PN977_005049"
     CDS             complement(3884..>4564)
                     /locus_tag="PN977_005049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001506261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03752 family integrating conjugative element
                     protein"
                     /protein_id="HDI6044965.1"
                     /translation="GTVTDPYPFKILVGRDNLTANGIELPDVEGAVVSGTASGDWTLS
                     CVRGQVNSITFVFADGTVRTLPQADVNGTTNSNQQAKNGNNAGGIGWISDEDGIPCIS
                     GTRKSNASTYLPTIFGLAAAGAAGDALSEGQYTSQNNVNGITSTLTGSAGEAAQGKAL
                     SGGMNETADWVRQRYGQTFDAIYVPPGKTLAVHITRQLAIDYEERGRRVRYDFTLPGE
                     AGDRGGLD"
BASE COUNT          913 a         1600 c         1108 g          943 t
ORIGIN      
        1 aacgcactat ccatgataat gacaaccgga cgcgaagcag tataatatcg cgcttttatt
       61 tcactttcag ccataatact gctgtgcata aaagacattg ttttttatgt aaccgctgtg
      121 ttaaaggtta taagggcatg ttccggcagg ccagaattgg acccatactc cgtaaaccac
      181 ctgccagtaa catgccacca cgacacagtt gccagtagcg gcagtttttt caggcttcct
      241 gcccgccatc acacagccag agcagcaggt agaacagatc ggccgcatcc accacctgga
      301 tataccagcg cggatgccgg tattcctctt cagagggcca gctctcatgc ccccctactg
      361 tcagcagaag atgcacctcg ccacacgccc cttctgctgt cacggccaca ctgctgctct
      421 gccccacccg ctggggctga cacaccacac gatgcagcgt acccagcctg cgttcaagct
      481 gtgaccgcag gcgctgcaga tgcggttgcg ccagatccgc aacacccggt tttccctgcg
      541 gcggcagaaa aaacacgctg cccccgggca tgacctctgt catcacctcc ggtttcgggt
      601 atttcagaca catcatacct ccgcctccat ggacgacaga ccacgcagcc tgtccagctt
      661 acgcgccaca tggaacgcag cctccagttc gctgcactgg tgttccttca tcagcatacg
      721 ccgctcagcc ttctcctcct tctccgtcat gcccagcgcc agatagagac ttggcggcac
      781 cgcacggaac agcgcctcaa tctgcttcgc cagcaccacg ccttcggtat aacagccgga
      841 cagcttactc gccgacatca gcaccgcttt ctgctcctcc gtcagtgatt taaagcgtgc
      901 aatgttgttc acttcctccg gtggcatcgt cagacacacc caccattccg ccatattcag
      961 catcttctcc gacacatccg gataatcctt caggttctgt gtcgccagcc acagccacgc
     1021 tcccagctta cgccacatct tcacaacctt cgtcatgtac ggcgaaagca gcggattgac
     1081 cgtcacgata tgcgcctcat ccaccgcaaa cacgatgtca cgccccagat actgatcacg
     1141 ttcggcaatg ttattgatca tgttggtcat cgacaccatg gtcagcgcca tctgtgcctc
     1201 atacccctca cgggccagat gccccaggtc aatcagcgtc acatccgctt ccggccacag
     1261 ttcgccctcg cggttgaaca actccgcctc aaaactcccc ttctgggtga acatccccag
     1321 agactccgcc atctccgccg cctttgcatt gcgcacatcg ttacggctgc tgtcacggga
     1381 gatctcccac agggcggact gcaaatcctc cggcagcatc tgccgcccct cacggtatgt
     1441 ccggtgtgtg gccatcagca gcgcctcgcg gatcatcgcc ctgtccgcac gcttcagcgc
     1501 ggcctcttct ttcgggtccc ccccggtgat catcatcctg gctgagattt ccatctcgcc
     1561 cagaatgtct cgtttatcct ccccttcatc cccggcttca tcctcaatat caggcagtga
     1621 gtgctcatcc acgcactgca gatcctgccc ttcctcaacc agtttatgcg catctgcaaa
     1681 cggcggcagg gtcacaccgg ttcccggtct gacgctgatt ttgttcaccg acagcccgag
     1741 actctcaaaa taatcggcca gcagaccaaa cgagttcccc gcttcggcaa tgaacaggcg
     1801 gggacggtgt actgccatca cctgcgacag tgaagcacac agcgtggccg atttaccggc
     1861 ccccgttggt ccgaacagca gcagatgcgc gttctgggta cggtccagct tgttcagcgg
     1921 atcgaaagtc agcacatcac caccccggtt aaaaaaactg aaccccggat gtccggtccc
     1981 cgtatcccgg ccggtcaccg gcaacaatcc tgccatgtgc tgtacccaca tcagacgcgt
     2041 gtaccactgc tttttgtccg tatccggatc aaaacacatc ggcagcgccc gcagccagct
     2101 gttcagcggc cccacgtcaa actcaggccg caccggctgc agaccagccc ccagaagcac
     2161 cgtggtcagc tccacacgcc ggcgtttcag gctgtccata tcctctgccc gcagaagaaa
     2221 ggtgagccct gcccggtaca gcttgtgccg gttccccagc agctccttca ccaccttcac
     2281 gtcctgacgc gcccggatgg attccgtgtt ctcacccacc gcatttttcg acagccgggt
     2341 gaaacggtcc tccagggtgt cctggggctg caccacgatg gtcatgcaca tcaccgttcc
     2401 ctccggaaag gtgtccatca gcgcattcac cgttttttcc ccacggggca tctctccggt
     2461 gatggtcccc ggctccggcg gcgtacgcag cttttccacc accacggcac agtgcggcat
     2521 ggcgtccagc caccacaccc cgttctccgg atcggaatac ggcgggttaa accacaggct
     2581 ctcggcaaaa tccgtcatca ccggcatcac atcatcacca cgctcacgcg gatcaacccg
     2641 tgaggccatc cggtacagct cttcacgggc aatccagtcc ggtgccggat taaataaccg
     2701 cagcagccag ctgtgcacct gctgaccgtt catccgggta cagcggatac cggctccacc
     2761 cagtgcggac accacgcggt cgcagacctg gttcagcatc gccaccggcg acatcggatc
     2821 gttattgttc ttcccgatac gacggtaaac caccatccgg gttcgtcgct gctgtccccg
     2881 ccagggctga ccggtgacca gggtgtccgt aaacagtccc tcaggacggg caatccccct
     2941 gagatggcgg gccatttcac ccagccaggc ctccgtgaat gccgttccct gcgcgtgggg
     3001 tttcacatac cccttcagat agtccaggta cgcatccaca tcattttcat cctgacagta
     3061 gaactgcacc acccagggat gcgtgtcgta ctcatcaaaa ctgtcctgca gggcatcctc
     3121 caccatgtcc ctgatttgcg ccaggcgctc ttccgtacgc ccttccgttg cggccggcgt
     3181 caccgaatag accgctccca ccgacacccc gtcgtcaagc agcagacact gctcctcatc
     3241 cagatattcc acccatggca gaaaatcaat aaccgacgga ccggcatgat aaaccgccgc
     3301 ctcagcatca cgggtcatct gtcctggacg tcgcaggggc tgtgcccccg gaggacaggg
     3361 cggaacaaag gccgatgaag atgcccccgc ttctgcttcc ggtcgttccc tggtctctgc
     3421 tgtacctttt tcctcacggc gtctgaaaag cgagaatgtc attacagagc ctccgtacgc
     3481 tcacctggca gcgcatactg cgtcctgctg taaaaaggaa ataccgtgct gtagcccgga
     3541 accggcgacg taccgccggc cagatgcgga aacacataca tcaccatgtc gggattcggc
     3601 agacgcggaa actgctggct gatttcgctt tcctgcgtcc ggctgtagct gtacgcctcc
     3661 gcgttcatcc cctgctgctc cgttaccgta aggggacggc gcaacgtttc ccgggcggcc
     3721 agcgcatgcc gctgaccgcc acctgcggtt ttaccctgcc agagctccag catcgtctgc
     3781 tctccggcag gcagcagctc ttctttagag gtggcacagc ccgacagcac cagtgccaca
     3841 ctcatcacga acagtaatcg tttcacggac tcattctccc tgttcagtca aggccgcccc
     3901 ggtcgcccgc ttctcccggc agggtgaagt cataacgcac acgacgtcca cgctcttcat
     3961 aatcaatcgc cagctgacgg gtaatgtgaa cggccagcgt ttttcccggc ggcacataaa
     4021 tggcatcaaa ggtctgcccg taacgctgtc ttacccagtc cgcggtctca ttcatcccgc
     4081 cggaaagggc cttaccctgc gccgcttcac cggcactgcc ggtcagcgtc gaggtgatac
     4141 cgttgacatt gttctgcgag gtgtactgcc cctcactcag ggcatccccg gccgcgccgg
     4201 ctgcggccag cccgaatatc gtcggcaggt aggttgacgc attggattta cgggtgccgc
     4261 tgatacacgg gatcccatcc tcatccgaaa tccagccaat cccgcctgcg ttattcccgt
     4321 ttttcgcctg ctgattgctg ttggtggtac cgttcacatc cgcctgtggc agcgtgcgta
     4381 ccgtgccgtc agcaaacaca aaggtgatgc tgttcacctg tccgcgcaca caggaaagcg
     4441 tccagtcacc gctggccgtc cctgacacca cagccccctc cacatccggc agttcaatgc
     4501 cgtttgccgt gaggttatcg cgccccacga ggatcttgaa cggatacgga tcggtcaccg
     4561 tgcc
//
LOCUS       DAMZFT010000247         4505 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.247, whole genome shotgun sequence.
ACCESSION   DAMZFT010000247 DAMZFT010000000
VERSION     DAMZFT010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4505)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4505)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4505
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.247"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(<1..334)
                     /locus_tag="PN977_005050"
     CDS             complement(<1..334)
                     /locus_tag="PN977_005050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415433.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6044966.1"
                     /translation="MKITTVGVCIICGIFPLLILPQLPGTVTLAFLTLFACVLAFIPV
                     KTVRYIALTLLFFVWGILAAKQILWAGETLTGATQDAIVEITATDGMTTHYGQITHLQ
                     SRRIFPAPG"
     gene            complement(540..824)
                     /gene="ihfB"
                     /locus_tag="PN977_005051"
     CDS             complement(540..824)
                     /gene="ihfB"
                     /locus_tag="PN977_005051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HDI6044967.1"
                     /translation="MTKSELIERLATQQSHIPAKTVEDAVKEMLEHMASTLAQGERIE
                     IRGFGSFSLHYRAPRTGRNPKTGDKVELEGKYVPHFKPGKELRDRANIYG"
     gene            complement(984..2657)
                     /gene="rpsA"
                     /locus_tag="PN977_005052"
     CDS             complement(984..2657)
                     /gene="rpsA"
                     /locus_tag="PN977_005052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HDI6044968.1"
                     /translation="MTESFAQLFEESLKEIETRPGSIVRGVVVAIDKDVVLVDAGLKS
                     ESAIPAEQFKNAQGELEIQVGDEVDVALDAVEDGFGETLLSREKAKRHEAWITLEKAY
                     EDAETVTGVINGKVKGGFTVELNGIRAFLPGSLVDVRPVRDTLHLEGKELEFKVIKLD
                     QKRNNVVVSRRAVIESENSAERDQLLENLQEGMEVKGIVKNLTDYGAFVDLGGVDGLL
                     HITDMAWKRVKHPSEIVNVGDEITVKVLKFDRERTRVSLGLKQLGEDPWVAIAKRYPE
                     GTKLTGRVTNLTDYGCFVEIEEGVEGLVHVSEMDWTNKNIHPSKVVNVGDVVEVMVLD
                     IDEERRRISLGLKQCKANPWQQFAETHNKGDRVEGKIKSITDFGIFIGLDGGIDGLVH
                     LSDISWNVAGEEAVREYKKGDEIAAVVLQVDAERERISLGVKQLAEDPFNNWVALNKK
                     GAIVTGKVTAVDAKGATVELADGVEGYLRASEASRDRVEDATLVLSVGDEVEAKFTGV
                     DRKNRAISLSVRAKDEADEKDAIATVNKQEDANFSNNAMAEAFKAAKGE"
     gene            complement(2768..3451)
                     /gene="cmk"
                     /locus_tag="PN977_005053"
     CDS             complement(2768..3451)
                     /gene="cmk"
                     /locus_tag="PN977_005053"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HDI6044969.1"
                     /translation="MTAIAPVITIDGPSGAGKGTLCKAMAEALQWHLLDSGAIYRVLA
                     LAALHHHVDVASEDALVPLASHLDVRFVSTNGNLEVILEGEDVSGEIRTQEVANAASQ
                     VAAFPRVREALLRRQRAFRELPGLIADGRDMGTVVFPDAPVKIFLDASSEERAHRRML
                     QLQEKGFSVNFERLLAEIKERDDRDRNRAVAPLVPAADALVLDSTTLSIEQVIEKALQ
                     YARQKLALA"
     gene            complement(3624..4388)
                     /gene="ycaL"
                     /locus_tag="PN977_005054"
     CDS             complement(3624..4388)
                     /gene="ycaL"
                     /locus_tag="PN977_005054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase YcaL"
                     /protein_id="HDI6044970.1"
                     /translation="MKNTKLLLAIAASAALLTGCQNTHGIDTNMAISSGLNAYKAATL
                     SDADAKAIANQGCAEMDSGNQVASKSSKYGKRLAKIAKALGNNINGTPVNYKVYMTSD
                     VNAWAMANGCVRVYSGLMDMMNDNEIEGVLGHELGHVALGHSLAEMKASYAIVAARDA
                     ISATSGVASQLSRSQLGDIAEGAINAKYSRDKESEADDFSFDLLKKRGISTQGLVGSF
                     EKLASLDGGRTQSMFDSHPPSTERAQHIRDRIASGK"
BASE COUNT         1086 a         1174 c         1072 g         1173 t
ORIGIN      
        1 ggcctggcgc agggaatata cgtcgacttt gtagatgagt gatttgaccg taatgagtgg
       61 tcatgccgtc agtagcagtg atctcaacta ttgcatcctg cgtcgcgcca gttaaggttt
      121 ctcctgccca caaaatttgc tttgctgcta atatgcccca aacgaaaaac agcaacgtca
      181 gcgcgatata acggacggtt ttaacaggga taaatgccag tacacaggcg aagagagtca
      241 gaaacgcaag ggttactgtc cctggcaatt ggggcaaaat cagcaacgga aaaattccgc
      301 aaattatgca tacgccgact gtcgttattt tcatgcaccc tccctgttta tagaaagtgt
      361 gcaactttgt tgaacggttg tctggtgggt gttttgcgtt ccccgaggca cgttcagaag
      421 aatttaacga tagggtgaaa actgctcaaa actctgcgag gcagcttcca gttccagatt
      481 agagaaatga aaaaagcacc cgacaggtgc ttttctcgcg ttcaagtttg agtaaaaact
      541 taaccgtaaa tattggcgcg atcgcgcagt tctttacctg gtttaaagtg aggaacgtat
      601 tttccttcca gttctacttt atcgccagtc ttcggattac gtccggtacg tggtgcgcgg
      661 tagtgcaaag agaaactgcc gaaaccgcgg atttcaatac gctcgccctg cgcaagagtc
      721 gaggccatat gctccagcat ctcttttact gcatcttcaa ccgtcttggc gggaatgtgc
      781 gattgctggg tggcaagtct ttctatcaat tctgacttgg tcatgattcc tccggttcct
      841 taaaggcaaa ttaagcggct gctgttgctg cattgattaa gggcggccgt agccgccctt
      901 agtgcttgat tacaggacga aacctgcaat ctgtcaagta aactcaacaa acttcggaat
      961 aaaaatcccg aagagtcaga gaattactcg cctttagctg ctttgaaagc ttcagccatt
     1021 gcgttgttgg agaagtttgc atcttcctgt ttgttaacag ttgcgattgc atctttctcg
     1081 tcagcttcgt ctttcgcacg aacagacagg ctgattgcgc ggtttttacg atcaacgccg
     1141 gtgaatttag cttcaacttc gtcgccaacg ctcagaacca gggtagcgtc ttcaacgcgg
     1201 tcacgggatg cttcagaagc acgcaggtaa ccttcaacgc cgtcagccag ttctacggtt
     1261 gcgcctttag cgtcaactgc agttacttta ccggttacga tagcgccttt cttgttcaga
     1321 gcaacccagt tgttgaacgg atcttctgcg agctgtttaa cgcccaggga gatacgttca
     1381 cgttctgcgt caacctgcag aacaactgca gcgatttcgt cgcctttttt gtattcacga
     1441 actgcttctt cgcctgcaac gttccaggag atgtcagaca ggtgaaccag gccgtcgatg
     1501 ccgccgtcca ggccgatgaa gataccgaag tcagtgatag acttgatttt accttcaaca
     1561 cggtcaccct tgttgtgggt ttccgcgaac tgctgccacg ggttagcttt gcactgtttc
     1621 agacccaggg agatacgacg acgttcttcg tcgatatcca gaaccataac ttccactaca
     1681 tcgccaacgt taacaacttt ggacgggtgg atgtttttgt tggtccaatc catttcggaa
     1741 acgtgtacca ggccttcaac gccttcttcg atttcaacga agcagccgta gtcggtcagg
     1801 ttggtcacgc gaccagtcag tttggtacct tccggataac gtttagcgat agctacccac
     1861 ggatcttcgc ccagctgttt caggcccagg gatacacggg tacgttcgcg gtcgaacttc
     1921 agcactttaa cagtgatttc gtcgcccacg ttgacgattt cgctcggatg cttaacgcgt
     1981 ttccaggcca tatcagtgat gtgcagcagg ccgtcaacgc cgcccagatc aacgaatgca
     2041 ccgtagtcag tgaggttctt aacgatacct ttaacttcca tgccttcctg caggttttcc
     2101 agcagctgat cgcgctctgc gctgttttcg gattcgataa cggcacgacg agaaacaaca
     2161 acgttgttgc gcttctgatc cagcttgatt actttgaatt caagctcttt gccttccagg
     2221 tgcagagtgt cacgcaccgg acgaacgtct accagagaac ctggcaggaa cgcacgaata
     2281 ccgttcagct caacagtgaa gccgccctta actttgccgt tgataacacc ggtaacagtt
     2341 tcagcatctt cgtaagcttt ttccagcgtg atccaggctt cgtgacgttt agctttctca
     2401 cgggacagca gagtttcacc gaagccgtct tctactgcgt ccagagcaac gtcaacttcg
     2461 tcacctacct ggatttccag ctcgccctgg gcgtttttga actgctcagc cgggatggcg
     2521 gactcagatt tcagaccagc gtcaaccagt actacgtctt tgtcgatagc aacaacaacg
     2581 ccacgaacga tagaacccgg gcgggtttcg atttctttta aggactcttc aaagagttga
     2641 gcaaaagatt cagtcatgtt taatcttcag gtttatattt aacgtccacc tagctccatg
     2701 ccggatgggg ttgtttaaca tacccgctgt cattccattg caacgggggt actgcaaatt
     2761 cggtcgctta tgcgagagcc aatttctggc gcgcgtattg tagcgctttt tcaatcactt
     2821 gctcaatgct taaggtggtg gaatccaaca ctaaagcatc ggctgccgga accagtggcg
     2881 ctaccgctcg gttacgatcg cggtcgtcgc gttctttgat ctcggccaaa aggcgctcaa
     2941 agttaacact aaagcccttc tcctgcaact gtagcatgcg gcgatgcgca cgttcttccg
     3001 aggaggcgtc aaggaaaatt ttcactggcg catcagggaa taccaccgtt cccatgtcgc
     3061 ggccatcggc aatcaggcct ggtaattcgc gaaacgcgcg ttggcgacgc aataatgctt
     3121 cacgaacgcg tgggaatgcc gcgacttgtg aggcagcatt cgccacttcc tgagtacgaa
     3181 tttcgccgct gacatcttcc ccttcgagga tcacttccag attgccattg gtcgacacaa
     3241 aacgtacatc cagatgggat gccagcggca ccagcgcatc ttccgatgca acatcaacat
     3301 ggtgatgtaa tgccgccagt gccagtacgc gataaattgc acccgagtcc agcagatgcc
     3361 attgcaacgc ttccgccata gccttacaca aggtgccttt ccctgcaccg cttgggccat
     3421 caatggtaat aaccggggca attgccgtca tctttatctc cttaaaacag gcgtaccgtt
     3481 aacataaccg cgcgcattat acgcgccgac gccacaaatc gttaacgttg tgtgcaaaaa
     3541 ccagttttgt ctgtattcag atggggattt attgcgcaat tatagcgggc tggcagaaga
     3601 ccagcccgaa agatgacaat gatttactta ccagaggcga tacgatcacg gatgtgttgc
     3661 gcacgctctg ttgatggtgg gtgagagtca aacatggact gggtgcgacc gccatccagg
     3721 ctggccaatt tttcaaagct gccaaccagc ccctgggtgc tgatgccacg tttcttcaac
     3781 agatcaaagg agaaatcatc tgcttcggac tctttatcgc gggagtattt cgcattgata
     3841 gcgccttctg cgatatcacc taattgtgag cgagaaagct gggaagccac accgctggta
     3901 gcggaaatcg catcacgtgc ggcaacgatc gcataagaag ctttcatttc agccagcgag
     3961 tgacccaacg cgacgtggcc cagttcatga cccagaacgc cttcaatttc gttgtcgttc
     4021 atcatgtcca tcaggccact gtagacacga acacagccgt tcgccatcgc ccatgcgttg
     4081 acgtcgctgg tcatataaac cttatagttg accggcgtgc cattaatatt gttacccaat
     4141 gctttggcga tttttgccag acgtttaccg tacttgctgg atttacttgc gacttgattg
     4201 ccgctgtcca tttcggcaca gccctgattg gcaatcgctt ttgcatcagc atcgcttaac
     4261 gttgctgctt tataggcatt taaacctgag ctgatagcca tattggtatc aataccgtgg
     4321 gtattctgac accctgtcag taaagctgca gaggccgcaa tcgccagcag taatttagta
     4381 ttcttcatgt gaatcatccg ttgattaatc atatataaat attatgtatc aataacttac
     4441 attgacaaac gacgctacca ttaatcaaaa tgactcacaa ggtccgaaaa aaaacgccgg
     4501 caatg
//
LOCUS       DAMZFT010000248         4493 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.248, whole genome shotgun sequence.
ACCESSION   DAMZFT010000248 DAMZFT010000000
VERSION     DAMZFT010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4493)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4493)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4493
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.248"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..406
                     /gene="fimG"
                     /locus_tag="PN977_005055"
     CDS             <1..406
                     /gene="fimG"
                     /locus_tag="PN977_005055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type 1 fimbria minor subunit FimG"
                     /protein_id="HDI6044971.1"
                     /translation="VVAKPCTVSTTNATIDLGDLYSFSLVSAGAASAWHDVALELTHC
                     PVGTSRVTASFSGAADSTGYYKNQGTAQNIQLELQDDSGNTLNTGATKTVQVDDSSQS
                     AHFPLQVRALTVNGGATQGTIQAVISITYTYS"
     gene            426..1328
                     /gene="fimH"
                     /locus_tag="PN977_005056"
     CDS             426..1328
                     /gene="fimH"
                     /locus_tag="PN977_005056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria D-mannose specific adhesin FimH"
                     /protein_id="HDI6044972.1"
                     /translation="MKRVITLFAVLLMGWSVNAWSFACKTANGTAIPIGGGSANVYVN
                     LAPAVNVGQNLVVDLSTQIFCHNDYPETITDYVTLQRGSAYGGVLSNFSGTVKYSGSS
                     YPFPTTSETPRVVYNSRTDKPWPVALYLTPVSSAGGVAIKAGSLIAVLILRQTNNYNS
                     DDFQFVWNIYANNDVVVPTGGCDVSARDVTVTLPDYPGSVPIPLTVYCAKSQNLGYYL
                     SGTTADAGNSIFTNTASFSPAQGVGVQLTRNGTIIPANNTVSLGAVGTSAVSLGLTAN
                     YARTGGQVTAGNVQSIIGVTFVYQ"
     gene            complement(1571..2914)
                     /gene="gntP"
                     /locus_tag="PN977_005057"
     CDS             complement(1571..2914)
                     /gene="gntP"
                     /locus_tag="PN977_005057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate permease GntP"
                     /protein_id="HDI6044973.1"
                     /translation="MHVLNILWVVFGIGLMLVLNLKFKINSMVALLVAALSVGMLAGM
                     DLMSLLHTMKAGFGNTLGELAIIVVFGAVIGKLMVDSGAAHQIAHTLLARLGLRYVQL
                     SVIIIGLIFGLAMFYEVAFIMLAPLVIVIAAEAKIPFLKLAIPAVAAATTAHSLFPPQ
                     PGPVALVNAYGADMGMVYIYGVLVTIPSVICAGLILPKFLGNLERPTPSFLKADQPVD
                     MNNLPSFGVSILVPLIPAIIMISTTIANIWLVKDTPAWEVVNFIGSSPIAMFIAMVVA
                     FVLFGTARGHDMQWVMNAFESAVKSIAMVILIIGAGGVLKQTIIDTGIGDTIGMLMSH
                     GNISPYIMAWLITVLIRLATGQGVVSAMTAAGIISAAILDPATGQLVGVNPALLVLAT
                     AAGSNTLTHINDASFWLFKGYFDLSVKDTLKTWGLLELVNSVVGLIIVLIISMVA"
     gene            3254..4438
                     /gene="uxuA"
                     /locus_tag="PN977_005058"
     CDS             3254..4438
                     /gene="uxuA"
                     /locus_tag="PN977_005058"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HDI6044974.1"
                     /translation="MEQTWRWYGPNDPVSLADVRQAGATGVVTALHHIPNGEVWSVEE
                     ILKRKAIVEDAGLVWSVVESVPIHEDIKTHTGNYEQWIANYQQTLRNLAQCGIRTVCY
                     NFMPVLDWTRTDLEYVLPDGSKALRFDQIEFAAFEMHILKRPGAEADYTEEEIAQAAE
                     RFATMSDEDKARLTRNIIAGLPGAEEGYTLDQFRKHLELYKDIDKAKLRENFAVFLKA
                     IIPVAEEVGVRMAVHPDDPPRPILGLPRIVSTIEDMQWMVDTVNSMANGFTMCTGSYG
                     VRADNDLVDMIKQFGPRIYFTHLRSTMREDNPKTFHEAAHLNGDVDMYEVVKAIVEEE
                     HRRKAEGKEDLIPMRPDHGHQMLDDLKKKTNPGYSAIGRLKGLAEVRGVELAIQRAFF
                     SR"
BASE COUNT         1131 a         1199 c         1144 g         1019 t
ORIGIN      
        1 ggtcgtcgcc aaaccgtgca cagtttccac caccaatgcc acgattgatc tcggcgatct
       61 ttattctttc agtctcgtgt ctgccggtgc ggcatcggcc tggcatgatg ttgcgcttga
      121 gttgactcat tgtccggtgg gaacgtcgag ggtcactgcc agcttcagcg gggcagccga
      181 cagtaccgga tattataaaa accaggggac cgcgcaaaac atccagttag agctacagga
      241 tgacagtggc aacacattga atactggcgc aaccaaaaca gtgcaggtgg atgattcctc
      301 acaatcagcg cacttcccgt tacaggtcag agcattgacg gtaaatggcg gagccactca
      361 gggaaccatt caggcagtga ttagcatcac ctatacctac agctgaaccc aaagagatga
      421 ttgtaatgaa aagagttatt accctgtttg ctgtactgct gatgggctgg tcggtaaatg
      481 cctggtcatt cgcctgtaaa accgccaatg gtaccgcaat ccctattggc ggtggcagcg
      541 ccaatgttta tgtaaacctt gcgcctgccg tgaatgtggg gcaaaacctg gtcgtagatc
      601 tttcgacgca aatcttttgc cataacgatt acccggaaac cattacagac tatgtcacac
      661 tgcaacgagg ttcggcttat ggcggcgtgt tatctaattt ttccgggacc gtaaaatata
      721 gtggcagtag ctatccattt ccgaccacca gtgaaacgcc gcgcgttgtt tataattcga
      781 gaacggataa gccgtggccg gtggcgcttt atttgacgcc tgtgagcagt gcgggcgggg
      841 tggcgattaa agctggttca ttaattgccg tgcttatttt gcgacagacc aacaattata
      901 acagcgatga ttttcagttt gtgtggaata tttacgccaa taatgatgtg gtggtgccca
      961 ctggcggctg cgatgtttct gctcgtgatg tcaccgttac tctgccggac taccctggtt
     1021 cagtgccgat tcctcttacc gtttattgtg cgaaaagcca aaacctgggg tattacctct
     1081 ccggcacaac cgcagatgcg ggcaactcga ttttcaccaa taccgcctcg ttttcacccg
     1141 cgcagggcgt cggcgtacag ttgacgcgca acggtacgat tattccagcg aataacacgg
     1201 tatcgttagg agcagtaggg acttcggcgg taagtctggg attaacggca aattacgcac
     1261 gtaccggagg gcaggtgact gcagggaatg tgcaatcgat tattggcgtg acttttgttt
     1321 atcaataaag aaatcacagg acattgctaa tgctggtacg caatattacc tgaagctaaa
     1381 aacctgcacg ttagcccttt gtaggccaga taagacgcgt cagcgtcgca tctggcataa
     1441 acaaagcgca ctttgctggt ctgttcccct caccctaacc ctctccccgg aggagcgagg
     1501 ggactggccg ggcacatttt tagactttgt catcagtcag agcctgccat tggcaggctc
     1561 cggtgttctt ttacgctacc atgctaataa tcaacacaat aatcagccca accacggagt
     1621 tgaccagctc cagcagtccc caggttttca acgtgtcttt taccgacagg tcaaagtaac
     1681 ctttgaacag ccagaatgag gcatcattaa tgtgggtgag ggtgttggaa cccgcagccg
     1741 tcgccagtac cagcagcgcc ggattcacgc caaccagctg accggttgct ggatcaagga
     1801 ttgcagcact gataatcccg gcggcggtca tcgccgaaac gacaccctga cccgtcgcca
     1861 gacgaattag cacagtgatc agccatgcca tgatgtaggg cgagatattg ccgtgggaca
     1921 tcaacatgcc gatggtgtcg ccaatgccgg tgtcgatgat ggtctgcttc agcacgccac
     1981 ccgcaccgat gatcagaatc accattgcaa tactcttcac cgcgctttca aaagcgttca
     2041 tcacccactg catgtcatga ccacgcgcgg tgccaaagag tacgaatgca accaccatcg
     2101 caataaacat tgcaatcggc gaggaaccga taaagttaac cacttcccag gcaggggtat
     2161 cttttaccag ccagatattg gcgatggtgg tggagatcat aatgatcgct gggatcagcg
     2221 gcaccagaat cgaaacgccg aaagagggca gattattcat atctaccggt tgatctgctt
     2281 tcaggaatga tggcgttggg cgctcaagat tgccgaggaa cttcggcagg atcagacctg
     2341 cgcagattac acttgggatc gtcaccagta cgccatagat ataaaccatc cccatatccg
     2401 cgccataagc attcaccagc gccaccggac ccggctgcgg tgggaacagt gaatgtgctg
     2461 tagtggcagc tgctactgcc gggatcgcca gtttcaggaa cggaatttta gcttcggcgg
     2521 caataacaat aaccagcggc gctaacatga taaaggccac ttcataaaac atcgccagac
     2581 cgaaaatcag gccgatgata atcaccgaca gctgtacata gcgcagaccg agacgcgcca
     2641 gcagcgtatg tgctatctgg tgagccgcgc cggagtcgac catcaattta ccgatgaccg
     2701 caccgaacac cacgatgata gccagttccc ccagcgtgtt gccgaagccc gctttcatgg
     2761 tgtgcagcag cgacatcaaa tccatgcccg ccagcatccc gacggacagc gccgccacca
     2821 acaaagccac cattgaattg attttgaact tcaaattcag taccagcatc agaccaatgc
     2881 cgaataccac ccagagaatg ttaagcacat gcataacgtt ttaccttacc tggttgaacc
     2941 gttgttattt tgggcgacat gttatgtaaa ttggtcaacc attgttgcga tgaatgtcac
     3001 atcctctgat caataaccat cgattactct ttgctgcaat ttgcagcaac aaccatgaga
     3061 gtgaaattct tgtgatgtgg ttaaccaatt ttagaattcg ggttgacatg tcttaccaaa
     3121 aggtagaact tatacgccat ctcatccgat gcaacgccac ggctgcggtc tggttgttca
     3181 tccggatacc taaacaactc cggggctcca cgtctctttg ctgtggaacc cactatgtga
     3241 aagaggaaaa atcatggaac agacctggcg ctggtacggc ccaaacgatc cggtttcttt
     3301 agctgatgtc cgtcaggcgg gcgcaactgg cgtggttacc gcgctgcacc atatcccgaa
     3361 cggcgaagta tggtccgtag aagagatcct caaacgcaag gcgatcgttg aagacgcagg
     3421 cctggtgtgg tctgtcgttg aaagcgtacc aattcacgaa gatatcaaaa cccacactgg
     3481 caactatgag cagtggattg ctaactatca gcagaccctg cgcaacctgg cgcagtgcgg
     3541 cattcgcacc gtgtgctaca acttcatgcc ggtgctcgac tggacccgta ctgacctcga
     3601 atacgtgctg ccagacggct ccaaagctct gcgcttcgac cagatcgaat tcgctgcatt
     3661 cgaaatgcat atcctgaagc gtccaggcgc ggaagcggat tacaccgaag aagaaattgc
     3721 tcaggccgct gaacgcttcg ccactatgag cgacgaagac aaagcgcgtc tgacccgtaa
     3781 catcattgcc ggtctgccag gtgcggaaga agggtatacc ctcgaccagt tccgtaagca
     3841 cctggagctg tacaaagata tcgacaaagc caaactgcgc gaaaactttg ctgtcttcct
     3901 gaaagcgatt attccagttg ctgaagaagt tggcgtgcgt atggcggttc acccggacga
     3961 tccgccgcgc ccaatcctcg gcctgccgcg cattgtttct accattgaag atatgcagtg
     4021 gatggttgat accgtaaaca gcatggcgaa cggtttcacc atgtgcaccg gttcctacgg
     4081 cgtgcgtgct gacaacgatc tggttgatat gatcaagcag tttggtccgc gtatttactt
     4141 cacccatctg cgctccacca tgcgtgaaga taacccgaaa accttccacg aagcggcgca
     4201 cctgaacggt gacgttgata tgtacgaagt ggtgaaagcg attgttgaag aagaacaccg
     4261 tcgtaaagcg gaaggcaaag aagacctgat cccgatgcgt ccggaccacg gtcatcagat
     4321 gctggacgac ctgaagaaga aaaccaaccc aggttactcc gcaattggtc gtctgaaagg
     4381 cctggccgaa gttcgcggtg tcgaactggc gatccagcgc gctttcttta gccgttaata
     4441 tccaccggca tggctgcgcg ccgtgccggt tccttcttcc ttgccgtcac tct
//
LOCUS       DAMZFT010000249         4465 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.249, whole genome shotgun sequence.
ACCESSION   DAMZFT010000249 DAMZFT010000000
VERSION     DAMZFT010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4465)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4465)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4465
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.249"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..345
                     /locus_tag="PN977_005059"
     CDS             <1..345
                     /locus_tag="PN977_005059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001546734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GH32 C-terminal domain-containing protein"
                     /protein_id="HDI6044975.1"
                     /translation="HAPPVAIERLEMDIIPSGELHLNFGNALALHLNDDGIQLQRKSL
                     AGQEKLTRYWRGSVTSLKILCDSSSIEIFINNGEGVMSNRYFPHHPASLILQGESDVT
                     LHYWSLRACMVE"
     gene            371..1378
                     /locus_tag="PN977_005060"
     CDS             371..1378
                     /locus_tag="PN977_005060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDI6044976.1"
                     /translation="MRKTKRVTIKDIAELAGVSKATASLVLNGRGKELRVAQETRERV
                     LSIAQQQHYQPSIHARSLRDNRSHTIGLVVPEITNYGFAVFSHELETLCREAGVQLLI
                     SCTDENPSQETMVVNNMIARQVDGLIVASSMLHDNDYQKLSEQLPVVLFDRHMNGSTL
                     PLVITDSVSPTAALVADIARSHPDEFYFLGGQPRLSPTRDRLEGFTQGLQQAGVTLQP
                     EWIIHGNYHPSSGYEMFAALCARLGRPPKALFTAACGLLEGVLRYMSQYNLLDSKIHL
                     ASFDDHYLYDSLSVRIDTIQQDNRQLAFHCFELISQLIEGETPSPLQRYLPASLQKRY
                     R"
     gene            complement(1464..2135)
                     /gene="queE"
                     /locus_tag="PN977_005061"
     CDS             complement(1464..2135)
                     /gene="queE"
                     /locus_tag="PN977_005061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HDI6044977.1"
                     /translation="MQYPINEMFQTLQGEGYFTGVPAIFIRLQGCPVGCAWCDTKHTW
                     EKLEDREVSLFSILAKTKESDKWGAASSEDLLAVIGRQGYTARHVVITGGEPCIHDLL
                     PLTDLLEKNGFSCQIETSGTHEVRCTPNTWVTVSPKLNMRGGYEVLSQALERANEIKH
                     PVGRVRDIEALDELLATLTDDKPRVIALQPISQKDDATRLCIETCIARNWRLSMQTHK
                     YLNIA"
     gene            2308..2910
                     /locus_tag="PN977_005062"
     CDS             2308..2910
                     /locus_tag="PN977_005062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000944219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HDI6044978.1"
                     /translation="MLRIFIVIIFIVNLSGCGYNDIQTYDEQVNASWSEVLNQYQRRA
                     DLIPNLVASIKGYSSHEQEVLEAVTLARSQANRASSDLQQAPGDAQKLQAWQQAQAQV
                     SRTLGQLTIISERYPELKAQELYQNLMVQLEGSENRIAVARGRYIKAIEQYNVTIRKF
                     PAVLTAKVMDYTPKKNYLPDDVTAVSKAPTIDFSQNANAH"
     gene            2924..3817
                     /locus_tag="PN977_005063"
     CDS             2924..3817
                     /locus_tag="PN977_005063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001243480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgcG family protein"
                     /protein_id="HDI6044979.1"
                     /translation="MRNFIFLMAFFCSSVFATQIPVPESPKYVNDLTGTLTNSEVNTL
                     TNQIKALTQKNHAQLVVLVVDTTGDETIEQYATRVFDSWKPGDKDRDDGVLLLVAWQD
                     HTVRIEIGYGLEGIITDVQSGKIIRNSIIPAFKKGDLAGGLQKGINDIESRLTGNNSA
                     TITPTDHPLPFSGWWALLVWAIILTFISARGYIKTLGLICFAAIVLAFVLPIAGFSGS
                     WGVLATLLCFATPFLAVAIAFTPFGKKVRDSMRNTNQPSRHTRSNSSSHYSSSSSSSF
                     DNDNFRGGGGSSGGGGASGRW"
     gene            3832..>4465
                     /locus_tag="PN977_005064"
     CDS             3832..>4465
                     /locus_tag="PN977_005064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgcG family protein"
                     /protein_id="HDI6044980.1"
                     /translation="MRIFLLLLMLYGFQAFAEQLPSVTDLTGTLTTEEQTALNQQLQT
                     LEQQNHFQVAVLVTPTTGGKNIKLAASQSYGLYHWKPVGEKRYGEGILILVVWPEGLA
                     SMKIGHGLKEMLPPEQAAQIVHYHMQPEFKKNNLFSGLTQGIEGIAQFTYIKANLSPL
                     DALANHLFANPQRSLPCLAWTLLMIVAIVVLWRFTSRPGPGIWMISMITPM"
BASE COUNT         1209 a         1069 c         1022 g         1165 t
ORIGIN      
        1 catgcgccgc ctgtcgcaat tgaacgtctg gaaatggaca tcattccatc aggcgagcta
       61 catctgaatt ttggcaacgc actggcactg catcttaatg acgatggtat tcaactacag
      121 aggaaaagcc ttgcagggca agagaaacta actcgttact ggcgaggaag cgtaacgtca
      181 ctgaagattc tctgtgacag ttccagcata gagatattca tcaataatgg cgaaggcgta
      241 atgagcaatc gttattttcc ccatcatcca gcttcgctga tattacaggg cgagtctgac
      301 gtcacgttac actactggtc gctacgcgcc tgcatggtag aatgagggtt ttgaattacg
      361 gatcctcgtc gtgagaaaaa ccaaacgcgt tactattaaa gacattgctg aactggcggg
      421 agtgtcgaaa gcaaccgcca gtctggttct caatggtcgt ggcaaagagt tacgtgttgc
      481 gcaggaaacg cgtgagcgtg tgctgagtat tgcccaacag cagcattatc agcccagcat
      541 tcatgcccgc tcattacggg acaaccgcag tcacaccatt ggtctcgtcg ttcctgaaat
      601 cactaactac ggatttgcag tattttccca tgaactggaa acattgtgtc gggaagcggg
      661 agtccaactc ctcatatcct gtactgatga aaatccttca caggaaacaa tggtggtgaa
      721 caatatgatt gcccgccagg ttgatgggtt aatcgtcgca tccagtatgc tgcatgataa
      781 tgactatcaa aaacttagcg agcaattacc tgttgtgttg ttcgatcgcc acatgaacgg
      841 cagcacattg ccattggtca ttactgactc tgtttctccc accgccgctc tggtggcaga
      901 tattgcacgg agtcacccgg atgaattcta ttttctgggc ggacaacccc gtttgtcacc
      961 gactcgcgat cgactggaag gatttacaca aggcttgcag caagccggag taacgctaca
     1021 acctgaatgg attattcacg gtaattatca tccgagctca ggctatgaaa tgtttgccgc
     1081 gctatgcgct cgtttaggac gtccccctaa agccttattc actgccgctt gtggattact
     1141 ggaaggcgtt ctgcgctata tgagccaata taatttactc gacagtaaaa tccacctggc
     1201 gagttttgat gatcattact tgtatgattc actttcggta agaatcgaca caatacaaca
     1261 ggataatcgc caacttgctt ttcactgctt tgaacttatt tcgcaactga ttgaaggtga
     1321 aacgccatcc ccattacagc gttatcttcc tgccagtcta caaaaacgct accgataatc
     1381 ccgtcactat cttgcatcgc ccctgtaaaa cggggcgatt gtttttcaac ctgaacgtta
     1441 aatattaatg atgaataatc aaatcaggca atatttagat atttatgtgt ttgcatcgac
     1501 aaacgccaat tacgcgcaat gcaggtttca atgcacaaac gtgtggcatc atccttttgg
     1561 ctaatcggct ggagtgcaat gactcgcggt ttatcatcgg tcagcgttgc cagtagttca
     1621 tccagtgctt caatatcacg tacgcgtccc actggatgct tgatttcgtt ggctcgctcc
     1681 agtgcctgtg acaacacttc atagccgccg cgcatgttca gctttggcga tacggtaacc
     1741 caggtattcg gcgtgcagcg tacttcatga gtaccgctgg tttcgatctg gcagctaaaa
     1801 ccgttctttt cgagcaggtc agtcagtggc agcaaatcat gaatgcaagg ctcaccaccc
     1861 gtaatcacca catgccgcgc ggtatatccc tgccgaccaa tgacagccag caaatcttca
     1921 ctgctcgccg ccccccactt atcactctct ttggtcttcg ccagaatgct gaaaagggag
     1981 acttcccgat cctcaagctt ttcccaggtg tgtttggtgt cgcaccaggc acagccaacc
     2041 gggcatccct gtaaacgaat aaaaatggcg gggacgccgg taaagtaacc ctcaccttgc
     2101 agggtctgga acatctcgtt aatcgggtac tgcatagcat tctctgtgaa gtggataatt
     2161 gttaattatt gcagatcctg ccacaacaat catgtcttat taacattctg ttacaggcag
     2221 gtttaaggca aaaagtggat gagatcataa agaataaaat gaagcggcgt attatgtttt
     2281 aaggaattaa ttaaaaggga catagacatg ctcaggatat tcatcgtaat tatatttatt
     2341 gtcaatttat caggctgtgg atataacgat attcaaacct atgatgagca ggttaacgcc
     2401 tcctggtctg aggtattgaa tcaatatcaa cgtcgcgccg atcttattcc taatcttgtc
     2461 gccagtatta aaggttattc tagccatgaa caagaggtac tggaagccgt aacattggca
     2521 cgtagccagg ctaatcgcgc cagtagcgat cttcagcaag ctccgggaga tgcgcaaaaa
     2581 ttacaagcct ggcaacaagc ccaggcgcag gtgtctcgca ctctcggtca gttaacgata
     2641 attagtgaac gttaccctga gttaaaagct caggaactct atcaaaactt aatggtacag
     2701 ctcgaaggaa gtgaaaatag aattgccgtt gcgcgaggaa gatatattaa agccattgaa
     2761 caatataatg tcactatccg caaattcccc gctgtattaa ccgcaaaggt catggattac
     2821 acaccaaaga aaaattattt gccggatgat gtcaccgcag taagtaaagc cccaacaata
     2881 gattttagcc agaatgctaa tgcgcattaa gcggggaagt tctatgcgaa attttatctt
     2941 ccttatggct ttcttctgtt catctgtgtt tgccacacaa attccagtac ctgaatcccc
     3001 caagtatgtg aatgacttaa ccggcacatt aacaaacagc gaagtaaaca ccctgaccaa
     3061 ccagattaaa gccctgacgc aaaaaaacca cgcacagttg gtcgtgttag tggttgatac
     3121 aacaggcgat gaaaccatcg agcagtacgc aacgcgggta ttcgatagct ggaaaccggg
     3181 tgataaagac cgcgatgatg gcgttctact gttagtcgcc tggcaggatc ataccgtgcg
     3241 tattgaaatc ggttacggac tggaaggtat tattacggat gtacagtccg ggaaaattat
     3301 tcgcaacagt attattccgg catttaaaaa aggcgacctg gctggtggtt tacaaaaggg
     3361 gatcaacgat attgaaagtc gcctgacggg gaataattca gcaacgataa caccgaccga
     3421 tcaccctttg ccctttagcg gttggtgggc tttgttagtc tgggcgataa tactgacgtt
     3481 tatttctgcc agaggatata tcaaaacgct gggactgatt tgcttcgcag ctattgtact
     3541 ggcctttgtt ttacctatag caggtttcag cggaagctgg ggagtgctcg caacattact
     3601 ctgcttcgcc acaccgttcc tggctgtcgc catcgctttt actcccttcg gcaaaaaggt
     3661 tcgggattcg atgcgtaata ccaaccagcc atcacgccat acaagaagta attcttcctc
     3721 ccactattcg tcatcgtcat cctcttcatt cgacaatgac aatttccgcg gcggcggcgg
     3781 ttcatctggc ggcggtggcg cctccgggcg ctggtaacaa aaggaattga aatgcgtatt
     3841 tttctcttac ttctgatgct atatggtttc caggcattcg ctgaacaatt gccttcagta
     3901 actgacttaa ccgggacatt aacgacagaa gaacaaactg ctttaaatca acaattacag
     3961 actctcgagc agcaaaacca cttccaggtg gccgtgttgg ttacgccgac cacagggggc
     4021 aaaaacatta aacttgcagc ctctcagagc tacgggttat atcactggaa acccgttgga
     4081 gagaagcggt acggcgaagg tatcttaatt ctcgtcgttt ggccggaagg gctggcgtca
     4141 atgaagattg ggcatggact gaaagagatg ctgccaccgg aacaagctgc gcaaattgtt
     4201 cattaccata tgcaaccgga attcaaaaaa aataatctct tctccgggtt aacgcaggga
     4261 attgaaggca tcgcacagtt tacctatata aaagcgaatc ttagcccttt ggatgcttta
     4321 gccaatcatc tgtttgctaa tccgcaacgc tcgctgccat gtcttgcctg gacattactg
     4381 atgatcgttg ccatcgttgt cctctggcga tttaccagcc gtcccgggcc tggcatatgg
     4441 atgatatcga tgattactcc tatgg
//
LOCUS       DAMZFT010000250         4403 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.250, whole genome shotgun sequence.
ACCESSION   DAMZFT010000250 DAMZFT010000000
VERSION     DAMZFT010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4403)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4403)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4403
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.250"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            140..475
                     /locus_tag="PN977_005065"
     CDS             140..475
                     /locus_tag="PN977_005065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000157082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein MobC"
                     /protein_id="HDI6044981.1"
                     /translation="MASKKFYSDDDIQLAKSALSELPDLTAQRKTLRDFLDAIRDDII
                     VLVRTKGYTLADVRDTLKNAGYEVGEKALRDIIREAESKKPSRRSSSKTAPKKTDSGR
                     KDGIDMNNN"
     gene            complement(561..1409)
                     /locus_tag="PN977_005066"
     CDS             complement(561..1409)
                     /locus_tag="PN977_005066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001401764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
                     /protein_id="HDI6044982.1"
                     /translation="MNQTLPTADLNTAGTTDVIPSVAIDRIIAQRNEGITLFMQAMEC
                     LATARRILLDASGDIFLYGFEDCVTDSVRRMDKPEEAKRNITRLADRKIWDRLMTDTG
                     MYTFMSSCQRDEWNSQLMSDTCPEITLDNVLATFRHLNASKMQTFEQGLIDVYRKLSW
                     DYRTNNPCRLGKKIIIENLLYRWSNGRVTLDCSGREALDDLVRPFYLLEGRNVPDFRS
                     SIGAQYCEFLGNGDNIGELFEGEYFTVRGYQKGTVHIVFKRSDLVEKLNDIIARHYPG
                     ALPPRV"
     gene            1758..1955
                     /locus_tag="PN977_005067"
     CDS             1758..1955
                     /locus_tag="PN977_005067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001753860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6044983.1"
                     /translation="MQGAPHPARLKKNDGTTMACGGHWHTTPAQVKTEKTARHLQPKK
                     VKFLHFFTLIYRADLMVMMIK"
     gene            1989..2240
                     /locus_tag="PN977_005068"
     CDS             1989..2240
                     /locus_tag="PN977_005068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003980153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDI6044984.1"
                     /translation="MTGWELRIWRKSMLWSREKAAREFGVTQRTWHAWENAEQVDVTV
                     WRTTQALSVRDLLPHMQGMRKADIIRRLENELGETAEDV"
     gene            complement(2271..2468)
                     /locus_tag="PN977_005069"
     CDS             complement(2271..2468)
                     /locus_tag="PN977_005069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001348086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6044985.1"
                     /translation="MPGFFVSRSRANSMTIAERLIQKGALEVAREIACRLRDMGWTPE
                     RIQEATGLSGEELKKLFPDEL"
     gene            complement(2491..3408)
                     /locus_tag="PN977_005070"
     CDS             complement(2491..3408)
                     /locus_tag="PN977_005070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HDI6044986.1"
                     /translation="MSKKKNTTTPTPHDAAFRSFLANPDVARDFLELHLPAEYRQLCD
                     LSTLKLEPATFVEPDLHQYASDILWSVKTTGGEDGYVYTLIEHQSTENLYMPFRMLRY
                     SVAAMQRHLEQHKTLPLVIPVLFYHGERSPYPYSMNWLDCFENPALASKIYTKPFPLV
                     DITVVDDNEIMNHRRMAALTLLMKHIRQRDMLMCLDNLVRALQDIQDEEQITVLFNYL
                     LNGSEHVTVKFLQTLAQRLPQHEDSIMTLAERLKQEGIQQGIQQGIQKGRMEEKLDIA
                     RQLQKTGMSFAQICQITGLSEAELKKIAH"
     gene            complement(3473..3739)
                     /locus_tag="PN977_005071"
     CDS             complement(3473..3739)
                     /locus_tag="PN977_005071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001247860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6044987.1"
                     /translation="MRPSIIFATAEYVKRLREECLRENKPLHRHTRFRRQELAQDEIN
                     PDVLAMSGHIARRCSEQKRVRIPAMKVSEWGHLLRALEIERVCH"
     gene            complement(3831..4265)
                     /locus_tag="PN977_005072"
     CDS             complement(3831..4265)
                     /locus_tag="PN977_005072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000218847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="post-segregation killing protein PndC"
                     /protein_id="HDI6044988.1"
                     /translation="MTVSSTISVFCRDGVFRTVYCHLHGEPTWNGRILHTHYATGQLA
                     EALVEHGDIRCLGPRCDKPAGHTLQNPVDGVTAYYGRDSGFRMDSEAREYRSFREAIA
                     TESTEEVRFHYVFIDGYWKVMYRTPEGWKMKALALALRRCPK"
BASE COUNT         1027 a         1179 c         1141 g         1056 t
ORIGIN      
        1 ccgtttttca tggcattata gcctgttatt tttgtgcatc atagtcttat gcagacggca
       61 gaggcagggc gcagccctgc caggagtcag tttttgatga taaaatcagg cttcaacatg
      121 aaacggagag acagtgccaa tggcttctaa aaaattttac tcagatgatg acatccagct
      181 ggcaaaatct gccttatcgg aattgccgga cctcaccgct caacggaaaa cattgcgtga
      241 ttttcttgat gccattcgtg atgacatcat tgtcctggta aggacaaagg gttatacact
      301 tgcagatgtt cgtgacacgc tcaaaaatgc agggtatgaa gtaggagaga aggcacttcg
      361 tgacattatt cgggaagcag aatcgaaaaa accatcccgc cgttcttcct ctaaaacagc
      421 acctaaaaag acagattcag gcaggaaaga tggcatcgat atgaacaaca attgatgtga
      481 caatgtcaca tcgtgttctg agattatgat gattacagaa aaaaaagccc cggcataaat
      541 accagggctt ttctgtttaa tcagactcgt ggcggcaatg caccaggata atgccgtgca
      601 ataatatcat tcagtttttc aacaaggtca gaacgcttaa agacaatgtg tacggttcct
      661 ttctggtagc cacgcaccgt aaaatattcc ccttcaaaca actcaccgat attgtcgccg
      721 ttcccgagaa attcacaata ctgcgccccg atgctgctcc ggaagtcagg aacgttgcgc
      781 ccctccagca gataaaacgg acgtaccagg tcatccagtg cctcccgtcc gctgcagtcc
      841 agcgtcacac gcccgttact ccagcggtac agcaggtttt caataatgat tttcttgccc
      901 agacggcagg gattattggt tctgtaatcc catgacaatt tccggtagac atcaatcagt
      961 ccctgttcaa atgtctgcat tttgctggca ttcagatggc ggaatgttgc cagtacattg
     1021 tccagggtga tttcaggaca ggtgtcgctc atcagctggc tgttccactc atcacgctgg
     1081 catgaactca tgaaggtgta catgcccgta tctgtcatca ggcggtccca gattttccgg
     1141 tcggcaagac gggtgatatt ccttttcgct tcttccggtt tatccatgcg acgaacggaa
     1201 tcagtcacgc agtcttcaaa cccgtaaaga aaaatatcac ctgacgcatc gagcagaatc
     1261 ctgcgcgctg tcgccaggca ttccatcgcc tgcatgaaca gtgtaatacc ttcgttacgc
     1321 tgcgcgatga tgcggtcgat agccacagac ggaataacat ctgtcgtgcc ggcagtattc
     1381 aggtcagcag tgggtaaagt ctgattcatc acattgtctc ctcatcgtca cgactgccgg
     1441 gtcagccagg tcatttcccg gaggagcgaa gctccttctg ccgtccgggt cttcggggat
     1501 ttccccgcag tggacctttc cgacggggga cgctcaccac gtcacggggc agttgtccgg
     1561 gggcagacgc aaaagttttt gacgcctgcg gcaaaaagtt ttgtggctgg tacatctctc
     1621 ccctggacga ctgaaacggg atgtggtcgt gtatgtaccc ccggaaagtc cgtctgcgga
     1681 aaacgggccg cagtgcggcg gcagacaggc agagacagct gcgccatcag gcgcagtggc
     1741 cttacacacc gtcaggtgtg cagggagcgc cgcatccggc gcgactgaag aaaaacgacg
     1801 gcacaacaat ggcatgtggt ggtcactggc acaccactcc cgcacaggtg aaaacagaga
     1861 aaaccgcacg acatttacaa ccaaaaaaag tgaaatttct tcactttttc actcttattt
     1921 atcgcgctga cctgatggtt atgatgataa aataaagtga agttttttca ctttttcaaa
     1981 ggtgttttat gaccggctgg gaactgcgaa tctggcgaaa aagcatgctc tggtcacgcg
     2041 aaaaagcggc cagagaattt ggcgtgacgc agagaacatg gcacgcctgg gaaaatgcgg
     2101 aacaggtgga tgtcaccgtc tggaggacca cccaggcact ttcagtccgt gacctgctgc
     2161 ctcacatgca ggggatgcgt aaggcagata tcatcaggcg actggaaaat gaactggggg
     2221 aaacggcaga ggatgtgtga gcgtgattca cagcctggca gaatgccagg ctacagctca
     2281 tcaggaaaca gttttttcag ttcttcaccg gaaagtccgg ttgcctcctg aatccgttcc
     2341 ggcgtccagc ccatatcccg aagccggcag gctatttccc gcgccacttc aagagcgcct
     2401 ttttgaataa gacgttctgc aatcgtcatg ctgtttgctc tgctcctgct gacaaaaaag
     2461 cccggcattt tgccgggctg tgatgtttac tcagtgggcg atttttttca gttcggcttc
     2521 agaaagtccg gtgatctgac agatttgtgc aaatgacatt cccgttttct gaagctgacg
     2581 cgcaatatcc agcttctcct ccatgcggcc tttctgtatt ccctgctgta ttccctgctg
     2641 tattccttcc tgcttaagac gttctgccag tgtcataatg ctgtcctcgt gctgcggcag
     2701 tcgctgcgcc agcgtctgca aaaatttaac cgttacatgt tcgctgccat taagcagata
     2761 gttgaaaaga actgtaatct gctcttcatc ctggatgtcc tgcagtgccc tgaccaggtt
     2821 gtccagacac atcagcatat cccgctgacg gatatgcttc atcagcagcg tcagtgcggc
     2881 catccggcga tggttcatga tttcattgtc atcaacgaca gtgatatcaa ccagcggaaa
     2941 cggctttgtg tatattttag acgcaagtgc cggattctca aaacagtcca gccagttcat
     3001 gctgtacggg tacgggctgc gctcaccatg atagaacagc accggaatca ccagtggcaa
     3061 cgttttgtgc tgctccagat gtctctgcat cgccgccaca ctgtagcgta acatgcggaa
     3121 aggcatgtac agattttcgg tgctctggtg ctcgatgagc gtataaacat agccgtcttc
     3181 acccccggtg gttttcacac tccagaggat atcgctggcg tactgatgca ggtccggctc
     3241 aacaaaggtg gcgggttcca gcttcagggt ggacaggtcg cacaactgcc ggtactccgc
     3301 aggaagatgc aactccagaa aatccctggc aacgtcggga ttcgccagga acgaccggaa
     3361 cgcggcatca tgcggcgtgg gcgttgtggt gtttttcttc ttactcacgg ttcctcctgg
     3421 tgagatgtct gaacgtatcc tgccgcctgc acaggcagac ggcaagggtt aatcagtgac
     3481 agacccgttc aatttcaagc gcgcggagca ggtggcccca ttcgctgact ttcatggcgg
     3541 ggatacgcac ccgcttctgc tcactgcagc gtctggcgat atgaccgctc atcgccagga
     3601 cgtccgggtt aatctcatcc tgtgccagct cctgacgtct gaagcgggta tgacggtgca
     3661 ggggtttatt ctcccgcagg cactcttcac gcagacgctt tacatactcg gcggttgcga
     3721 agataattga tggtctcaca aatatttctc cttatgttgt tgatgactgc cgctcttcct
     3781 ggcggggaag taacggcgga gagaggcagg gtttatcgcc ctgccttttt tcatttcgga
     3841 cagcgacgca gtgccagcgc gagcgctttc atcttccagc cttccggcgt gcggtacatc
     3901 accttccagt agccgtcgat gaacacataa tggaagcgca cctcttcagt gctttcagtg
     3961 gcaatagcct ccctgaaaga acggtactca cgcgcctcac tgtccatccg gaaaccactg
     4021 tcacgtccgt aataagccgt cacaccgtcc accgggttct gaagcgtatg cccggcgggt
     4081 ttgtcgcaac ggggaccgag gcaacggata tctccgtgtt caaccagggc ttcggccagc
     4141 tgaccggtgg catagtgggt atgcaggatg cgaccgttcc aggtcggctc accgtgcagg
     4201 tggcagtaaa cggtacggaa caccccgtct cggcaaaaaa cggaaatcgt gctgctgact
     4261 gtcatgaaaa cgtctccttc tgctgaatca ggtcgccggg ccgaccggtt gctcccccgg
     4321 aggggcgaaa gccccttctg ccgtccactt cttcggggtt tctccccgca gtggaccttt
     4381 ccgatggggc agcgactgcc acg
//
LOCUS       DAMZFT010000251         4372 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.251, whole genome shotgun sequence.
ACCESSION   DAMZFT010000251 DAMZFT010000000
VERSION     DAMZFT010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4372)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4372)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4372
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.251"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(<1..390)
                     /locus_tag="PN977_005073"
     CDS             complement(<1..390)
                     /locus_tag="PN977_005073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HDI6044989.1"
                     /translation="MRTEYCGQLRLSHVGQQVTLCGWVNRRRDLGSLIFIDMRDREGI
                     VQVFFDPDRADALKLASELRNEFCIQVTGTVRARDEKNINRDMATGEIEVLASSLTII
                     NRADVLPLDSNHVNTEEARLKYRYLDLR"
     gene            700..1266
                     /locus_tag="PN977_005074"
     CDS             700..1266
                     /locus_tag="PN977_005074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HDI6044990.1"
                     /translation="MLELNAKTTALVVIDLQEGILPFAGGPHTADEVVNRAGKLAAKF
                     RASGQPVFLVRVGWSADYAEALKQPVDAPSPAKVLPENWWQHPVALGATDSDIEIIKR
                     QWGAFYGTDLELQLRRRGIDTIVLCGISTNIGVESTARNAWELGFNLVIAEDACSAAS
                     AEQHNNSINHIYPRIARVRCVEEILNAL"
     gene            1263..2081
                     /locus_tag="PN977_005075"
     CDS             1263..2081
                     /locus_tag="PN977_005075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HDI6044991.1"
                     /translation="MIYIGLPQWSHPKWVRLGITSLEEYARHFNCVEGNTTLYALPKP
                     EVVLRWREQTTDDFRFCFKFPATISHQATLRHCDDLVTEFLTRMSPLAPRIGQYWLQL
                     PATFGPRELPALWHFLDSLPGEFNYGVEVRHPQFFAKGEEEQTLNRGLHQRGVNRVIL
                     DSRPVHAARPHSEAIRDAQRKKPKVPVHAVLTATNPLIRFIGSDDMTQNRELFQVWLQ
                     KLAQWHQTTTPYLFLHTPDIAQAPELVHTLWEDLRKTLPEIGAVPAIPQQSSLF"
     gene            2134..2529
                     /locus_tag="PN977_005076"
     CDS             2134..2529
                     /locus_tag="PN977_005076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HDI6044992.1"
                     /translation="MVSALYAVLSALLLMKFSFDVVRLRMQYRVAYGDGGFSELQSAI
                     RIHGNAVEYIPIAIVLMLFMEMNGAETWMVHICGIVLLAGRLMHYYGFHHRLFRWRRS
                     GMSATWCALLLMVLANLWYMPWELVFSLR"
     gene            2570..3313
                     /gene="cmoA"
                     /locus_tag="PN977_005077"
     CDS             2570..3313
                     /gene="cmoA"
                     /locus_tag="PN977_005077"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy-S-adenosyl-L-methionine synthase CmoA"
                     /protein_id="HDI6044993.1"
                     /translation="MSHRDTLFSAPIARLGDWTFDERVAEVFPDMIQRSVPGYSNIIS
                     MIGMLAERFVQPGTQVYDLGCSLGAATLSVRRNIHHDNCKIIAIDNSPAMIERCRRHI
                     DAYKAPTPVDVIEGDIRDIAIENASMVVLNFTLQFLEPSERQALLDKIYQGLNPGGAL
                     VLSEKFSFEDAKVGELLFNMHHDFKRANGYSELEISQKRSMLENVMLTDSVETHKARL
                     HKAGFEHSELWFQCFNFGSLVALKAEDAA"
     gene            3310..4281
                     /gene="cmoB"
                     /locus_tag="PN977_005078"
     CDS             3310..4281
                     /gene="cmoB"
                     /locus_tag="PN977_005078"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-methoxyuridine(34)/uridine 5-oxyacetic
                     acid(34) synthase CmoB"
                     /protein_id="HDI6044994.1"
                     /translation="MIDFGNFYSLIAKNHLSHWLETLPAQIANWQREQQHGLFKQWSN
                     AVEFLPEIKPYRLDLLHSVTAESEEPLSAGQIKRIETLMRNLMPWRKGPFSLYGVNID
                     TEWRSDWKWDRVLPHLSDLTGRTILDVGCGSGYHMWRMIGAGAHLAVGIDPTQLFLCQ
                     FEAVRKLLGNDQRAHLLPLGIEQLPALKAFDTVFSMGVLYHRRSPLEHLWQLKDQLVN
                     EGELVLETLVIDGDENTVLVPGDRYAQMRNVYFIPSALALKNWLKKCGFVDIRIADVS
                     VTTTEEQRRTEWMVTESLADFLDPHDPSKTVEGYPAPKRAVLIARKP"
BASE COUNT          996 a         1101 c         1134 g         1141 t
ORIGIN      
        1 acgcaggtcg aggtagcggt atttcagacg cgcttcttcg gtgttgacgt ggttagagtc
       61 aagcggcaga acatctgcgc ggttgataat agtcagcgag gacgccagca cttcgatttc
      121 gccggtcgcc atatcgcggt taatattttt ttcgtcacgc gcacgtacgg tgcccgtgac
      181 ctgaatgcag aactcattac gcagttcaga ggccagcttt aacgcgtccg cacgatccgg
      241 atcgaaaaat acctgcacga taccttcgcg gtcgcgcata tcgatgaaga tcaggctacc
      301 aagatcacga cgacggttga cccaaccaca cagagtcacc tgctgcccca cgtgggacaa
      361 acggagctgt ccacaatatt ctgtacgcat gagatatccc ttaacttagc tgccggcgga
      421 tgccccctgc tgcgcaggtg accaagtcgc agcgttagct gtatgtcaca actgaatgaa
      481 aaaaggcggc tattatactg gaaattctgc cgcaccgtaa gagcctggcc cgcgctggaa
      541 cgcctcgtta ccactttata tcgggcctga aatcagactc tacgccagtt tgctataaag
      601 gtgttgcccg aactcataaa aattaacaaa atttgtcgtt ccgccatcgg ctaatcgcat
      661 taaggtgaga ggcacgattt tgttttgtca ggagtcatca tgcttgaact taatgctaaa
      721 accaccgcgc tggtggtgat tgatttacaa gaaggcatct taccttttgc cggtggtcca
      781 catactgccg atgaggtggt taatcgcgcc gggaagctgg cggcgaaatt tcgcgccagc
      841 ggtcagcccg tgtttctggt gcgtgttggc tggtctgccg attacgccga agcattaaaa
      901 cagccggttg atgccccttc ccccgctaaa gtgttgcccg aaaattggtg gcaacatcct
      961 gtagcattag gtgcaaccga cagcgatatc gaaatcatca aacgtcaatg gggtgcgttt
     1021 tacggtacgg atctggagtt gcaattacgc cgccggggta tcgataccat tgttttatgt
     1081 gggatctcga ccaatatcgg tgttgaatcc accgcccgca atgcctggga acttggcttt
     1141 aatctggtga ttgccgaaga tgcctgtagt gccgctagcg ccgagcagca caataacagt
     1201 atcaaccata tctacccgcg tattgcccgt gtgcgctgcg ttgaagagat cctcaacgca
     1261 ttatgattta catcggtctg ccgcaatggt cacatcctaa atgggtgcgg ttggggatca
     1321 ccagccttga agagtatgcc cgccacttta actgtgtgga gggtaacacc acgctttatg
     1381 ccctgccgaa acccgaggtt gtcctgcgct ggcgtgagca gaccacagat gacttccgct
     1441 tctgttttaa gtttccggcg accatttcgc atcaggcaac attacggcat tgcgatgatt
     1501 tagtgactga atttttgacc cgcatgtcac cgttggctcc gcgcattgga caatactggc
     1561 tgcaactgcc tgccacattc ggcccacggg agctgcctgc gctttggcat tttctcgact
     1621 ctcttcccgg cgaatttaat tatggtgtgg aagtccgcca tccacagttt ttcgccaaag
     1681 gggaagagga acaaacgctt aatcgcggtt tacatcagcg cggcgttaat cgggtgattt
     1741 tagacagccg cccggttcat gccgcacgtc cacacagtga agctattcgc gacgctcaac
     1801 gaaaaaaacc taaagttccg gtacatgctg tactgacggc gacaaatccg ctgatccgtt
     1861 ttatcggtag tgatgatatg acgcaaaacc gggaattatt tcaggtctgg ttacaaaaat
     1921 tagcgcagtg gcatcagacc actacgcctt atcttttttt acatacgcca gatattgccc
     1981 aggccccgga actggtacat accctgtggg aagacttacg taaaacgctt ccagagatcg
     2041 gagcagttcc ggctattcca cagcaatctt ctcttttctg aatttgccac ctatcataga
     2101 caggtgccat cggccatttt aaagggagtt tgtatggtaa gcgcgctgta tgccgtttta
     2161 agtgcgttgt tattaatgaa gttctctttt gatgtcgttc gcctgcgaat gcagtaccgc
     2221 gttgcctatg gcgacggcgg ttttagcgaa ctgcaaagcg ctattcgcat tcatggtaac
     2281 gcggtggaat atattcccat cgcgattgtg ttgatgctgt ttatggaaat gaatggcgca
     2341 gaaacctgga tggtgcatat ttgcggcatc gttttgcttg ctggtcgtct gatgcattat
     2401 tacggttttc atcaccgtct gttccgctgg cgacgttctg gcatgagcgc cacctggtgt
     2461 gcgctgttgc tgatggtgct ggcgaatctt tggtatatgc cctgggagtt ggttttctcc
     2521 ctgcgttagc gcacaatacg ccactttctt tttcccggat ttttacgtta tgtctcaccg
     2581 cgacacgcta ttttctgccc ctatcgccag actgggcgac tggacctttg atgaacgggt
     2641 agctgaagtc ttcccggata tgatccagcg ttccgttccc ggctattcca atattatttc
     2701 catgattggt atgttagccg agcgcttcgt tcaacctggt acgcaggttt acgatctggg
     2761 ttgttctctg ggcgcggcga cgctctcggt gcgtcgcaac attcatcatg ataattgcaa
     2821 aattattgcc atcgataact ccccggcgat gattgaacgc tgccgtcgtc atattgacgc
     2881 ctataaagct cctacgccag tagacgttat tgaaggtgat attcgcgata tcgccattga
     2941 aaatgcatcg atggtggtgc tgaattttac cctgcaattc ctggaacctt ccgagcgcca
     3001 ggcgttactg gataaaattt atcaaggact gaacccgggc ggcgctctgg tgctttcgga
     3061 aaaattcagt ttcgaagatg ccaaagttgg tgaactgctg ttcaacatgc accacgactt
     3121 taaacgtgcc aacggttaca gcgaactgga gatcagccag aaacgcagca tgctggaaaa
     3181 cgtgatgctg accgattccg tggaaaccca taaagcacgc ctgcataaag ccggttttga
     3241 gcatagcgag ctgtggttcc agtgctttaa ctttggttca ctggtggcat taaaagcaga
     3301 ggacgctgca tgatcgactt tggtaacttt tattctctga ttgccaaaaa tcatctttca
     3361 cactggctgg aaacgctgcc cgcgcagatt gctaactggc agcgcgaaca gcagcacggt
     3421 ctgtttaaac agtggtccaa cgcggtggaa tttctgcctg aaattaaacc gtatcgtctg
     3481 gatttattgc atagcgtaac cgccgaaagc gaagagccac tgagcgccgg gcaaattaaa
     3541 cgcattgaaa cgctgatgcg caacctgatg ccgtggcgca aagggccgtt ctcactgtat
     3601 ggcgtcaaca tcgataccga atggcgttcc gactggaaat gggatcgcgt tctgccccat
     3661 ctttctgatt taaccgggcg caccattctt gatgtcggct gcggcagcgg ttatcacatg
     3721 tggcgcatga ttggcgcagg cgcgcacctc gcggtgggaa tcgaccctac gcaactgttt
     3781 ctgtgccagt ttgaagcggt gcgtaaacta ctgggtaacg atcagcgcgc acatttgtta
     3841 ccgttaggta ttgaacagct tccggcactg aaagcctttg ataccgtctt ttcgatgggc
     3901 gtgctttatc atcgtcgttc accgctggag catctctggc agttgaaaga tcaactggtg
     3961 aatgaaggtg aactggtgct tgaaacgctg gttattgatg gtgatgagaa cacggtgctg
     4021 gtgccgggcg atcgttacgc gcaaatgcgt aatgtctatt tcatcccttc tgcgctggcg
     4081 ctgaaaaact ggctgaagaa gtgtggtttt gttgatattc gcattgcgga tgtgagcgtt
     4141 accaccacag aagaacagcg acgcaccgaa tggatggtta ctgaatcgct ggccgatttc
     4201 ctcgacccgc atgatccgag taagacagtt gaaggttatc cggcacctaa acgcgcggtg
     4261 ctgattgcgc gcaagccgta aagattagcg cccgtttccg ggcgcttttc acgtcttatg
     4321 cctgaacagc tggctgatca aacgccgtta acttcggtgc attgcccgta ta
//
LOCUS       DAMZFT010000252         4343 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.252, whole genome shotgun sequence.
ACCESSION   DAMZFT010000252 DAMZFT010000000
VERSION     DAMZFT010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4343)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4343)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4343
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.252"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(<1..2475)
                     /gene="torS"
                     /locus_tag="PN977_005079"
     CDS             complement(<1..2475)
                     /gene="torS"
                     /locus_tag="PN977_005079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system sensor histidine
                     kinase/response regulator TorS"
                     /protein_id="HDI6044995.1"
                     /translation="MNLTLTRRLWMGFALMALLTLTSTLVGWYNLRFISQVEKDNTQA
                     LIPTMNMARQLSEASAWELFAAQNLTSADNEKMWQAQGRMLTAQSLKINALLQALREQ
                     GFDTTAIELQEQEISRSLRQQGELVGQRLQLRQQQQQLSQQIVAAADEIARLAQGQAN
                     NAATSAGATQAGIYDLIEQDQRQAAESALDRLIDIDLEYVNQMNELRLSALRVQQMVM
                     NLGLEQIQKNAATLEHQLNNAVKILQRRQIRIEDPGVRAQVATTLTTVSQYSDLLALY
                     QQDSEISNRLQTLAQNNIAQFAQFSSEVSQLVDTIELRNQHGLAHLEKASARGQYSLL
                     LLGMVSLCALILILWRVVYRSVTRPLAEQTQALQRLLDGDIDSPFPETAGVRELDTIG
                     RLMDAFRSSVHALNRHREQLAAQVKARTAELQELVIEHRQARAEAEKASQAKSAFLAA
                     MSHEIRTPLYGILGTAQLLADNPALNTQRDDLRAITDSGESLLTILNDILDYSAIEAG
                     GKNVSVSDEPFEPRPLLESTLQLMSGRVKGRPIHLATAIADDVPTALMGDPRRIRQVI
                     TNLLSNALRFTDEGQIVLRSRTDGEQWLVEVEDSGCGIDPAKLAEIFQPFVQVSGKRG
                     GTGLGLTISSRLAQAMGGELSATSTPEVGSCFCLRLPLRVATAPVPKTVNQAVRLDGL
                     RLLLIEDNPLTQRITVEMLNTSGAQVVAVGNAAQALETLQNSEPFAAALVDFDLPDID
                     GITLARQLAQQYPSLVLIGFSAHVIDETLRQRTSSLFRGIIPKPVPREVLGQLLAHYL
                     QLQVNNDQPLDVSQLNEDAQLMGTEKI"
     gene            2558..3586
                     /gene="torT"
                     /locus_tag="PN977_005080"
     CDS             2558..3586
                     /gene="torT"
                     /locus_tag="PN977_005080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system periplasmic protein TorT"
                     /protein_id="HDI6044996.1"
                     /translation="MRALLLLLLSLFMLSAFSADNLLRWHDAQHFSVQASMPLKAKRA
                     WKLCALYPSLKDSYWLSLNYGMQEAARRYGVDLKVLEAGGYSQLATQQAQIDQCKQWG
                     AEAILLGSSTTSFPDLQKQVANLPVIELVNAIDAPHVKSRVGVPWFQMGYQPGRYLVQ
                     WSHGKPLNVLLMPGPDNAGGSKEMVEGFRAAIAGSPVRIVDIALGDNDIEIQRNLLQE
                     MLERHPEIDVVAGTAIAAEAAMGEGRNLKTPLTVVSFYLSHQVYRGLKRGRVIMAASD
                     QMVWQGELAVEQAIRQLQGQSVSDNVSPPILVLTPKNADREHIRRSLSPGGFRPVYYY
                     QHTSAAKK"
     gene            complement(3559..4251)
                     /gene="torR"
                     /locus_tag="PN977_005081"
     CDS             complement(3559..4251)
                     /gene="torR"
                     /locus_tag="PN977_005081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator TorR"
                     /protein_id="HDI6044997.1"
                     /translation="MPHHIVIVEDEPVTQARLQSYFTQEGYTVSVTASGAGLREIMQN
                     QPVDLILLDINLPDENGLMLTRALRERSTVGIILVTGRSDRIDRIVGLEMGADDYVTK
                     PLELRELVVRVKNLLWRIDLARQAQPHTQDNCYRFAGYCLNVSRHTLERDGEPIKLTR
                     AEYEMLVAFVTNPGEILSRERLLRMLSARRVENPDLRTVDVLIRRLRHKLSADLLVTQ
                     HGEGYFLAADVC"
BASE COUNT          960 a         1180 c         1154 g         1049 t
ORIGIN      
        1 gatcttctcc gtccccatca actgagcatc ttcattgagt tgcgatacat ccagcggttg
       61 atcgttattg acttgcagtt ggagatagtg cgccagtaat tgaccgagca cttcacgcgg
      121 caccggttta gggataatcc cgcgaaatag cgaactggta cgctgtcgca gtgtttcgtc
      181 aatgacatgg gcgctaaagc caatcaaaac cagcgacgga tattgctgtg ccagttgtcg
      241 ggcaagcgta atgccgtcga tatccggtag atcaaaatcc accagtgcgg cagcaaacgg
      301 ttcgctattt tgcagtgtct ctaaagcctg cgcggcattg ccaacagcaa caacctgcgc
      361 accactggtg ttcagcatct caacggtaat tcgctgggtt agcgggttat cttcaattaa
      421 cagcaaacgt aaaccgtcca gacgcaccgc ctgattgact gtttttggca cgggagcagt
      481 ggcaacacgt aacggcaagc gtaaacaaaa acagcttcca acctccggcg tgctggtggc
      541 gctcagttcg ccgcccatcg cctgggccag acggctactg atagtcagcc ccagcccggt
      601 gccgccgcgt ttgccgctta cctgcacaaa tggctggaag atttctgcca gtttcgcggg
      661 atcaataccg cagccgctgt cttccacttc gaccagccat tgctcgccat cagtgcggct
      721 acgtaaaacg atctgccctt cgtcagtaaa acgcagggcg ttgctcaaca ggttggttat
      781 aacctgacga atacgtcgcg gatcgcccat taacgcggtc ggtacatcgt cggcaattgc
      841 tgttgccagg tgaatcgggc gacctttaac ccgtccgctc attaattgca gggtactttc
      901 cagcagcggg cgcggttcaa agggctcatc gctgaccgaa acattcttgc cacccgcttc
      961 gatagccgaa taatcgagaa tatcgttgag gatggtcagc aacgattcgc cagaatcagt
     1021 aattgcccgc aaatcatcac gctgggtgtt aagtgccggg ttatctgcca gcagttgcgc
     1081 ggtgccgaga ataccgtaca gcggtgtgcg gatctcatgg ctcatcgccg ccagaaacgc
     1141 cgattttgcc tggctggctt tttctgcttc cgcccgtgcc tgtcggtgtt ctatcaccag
     1201 ttcctgcaat tcagctgtac gcgctttgac ctgcgccgcc agctgttcac ggtggcgatt
     1261 cagtgcatga acactgctgc gaaacgcatc catcagccgc ccgatggtat ccagctcccg
     1321 tacgccagcg gtttccggga aaggggagtc gatatcaccg tccagcagcc gttgcagcgc
     1381 ctgcgtttgt tcggcaagtg gacgcgtgac tgagcgataa accacgcgcc agaggatcag
     1441 aatcagtgcg caaagtgaaa ccatccccag caataacagg ctgtattgcc cgcgtgcact
     1501 ggctttttcc agatgcgcca gtccgtgctg attacgcagc tcaatggtgt cgaccagctg
     1561 actgacttcg ctactaaact gcgcgaactg ggcgatgtta ttttgggcga gagtttgcag
     1621 gcgattgctg atttcactgt cctgctgata cagcgccagc aaatcgctat attggctaac
     1681 ggtagttaac gttgttgcga cctgagcacg aactcccgga tcttcaatgc gtatttgccg
     1741 acgttgcaga attttcaccg cattattgag ctggtgttcc agcgttgctg catttttctg
     1801 gatctgctcc agccccagat tcatcaccat ttgctgcacc cgcagggcgc taaggcgcag
     1861 ttcattcatc tggttaacat actcaagatc gatatcaatc agccgatcga gtgcactttc
     1921 tgcggcctga cgctgatctt gttcgatcaa atcgtaaatc ccggcctggg tcgctccggc
     1981 ggaggttgcc gcattattcg cctgaccttg tgccaggcgt gcgatttcat cggcggcggc
     2041 gactatctgc tgactgagtt gctgttgttg ctggcgaagt tgcaaacgct gtcccaccag
     2101 ttccccttgc tgacgtaacg aacgggagat ctcctgctcc tgtagttcaa tagcggtggt
     2161 gtcaaaacct tgttcccgta acgcttgcag caacgcatta atcttcaggc tttgtgcggt
     2221 gagcattcgc ccctgcgcct gccacatctt ttcgttatcg gcactggtca ggttctgcgc
     2281 ggcgaaaagt tcccaggcgc tggcttcgct caactggcgc gccatattca tggtaggaat
     2341 caatgcctga gtgttgtctt tttccacctg gctgataaag cgcaggttgt accatcccac
     2401 cagggtactg gtcagggtta acagcgccat cagggcaaag cccatccaga gtcttcgggt
     2461 cagggttaaa ttcacggtcg gtgcacttaa ggtgaaaaag gttgagtcgc aaagcggaat
     2521 gcatctagca taaagcctta ttattgatga ggctatcatg cgcgcactgc tattgttact
     2581 tctttccctt ttcatgttat cggcattttc ggctgataac ctgttgcgct ggcatgatgc
     2641 gcagcatttc tcggtgcaag cctctatgcc gcttaaagcc aaacgcgcat ggaaactgtg
     2701 cgcgctttat cccagcctga aagattcata ctggttatcg ttgaactatg gtatgcagga
     2761 ggctgctcgc cgctacggtg tggatttaaa agtgctggag gcaggcggct acagccagtt
     2821 ggctacccag caagcacaaa tcgaccagtg taaacagtgg ggcgcagagg ccatcttgct
     2881 cggaagtagc acgacgtcat ttcccgacct gcaaaagcag gtagcaaatc tgccggtgat
     2941 cgaactggtg aatgctattg atgctcccca cgtgaaaagc cgcgttggtg tgccctggtt
     3001 tcagatgggc tatcaaccag gacgatatct ggtgcaatgg agccacggta aaccactgaa
     3061 cgtgctgttg atgcccggcc ccgataacgc cgggggcagt aaggagatgg tagagggttt
     3121 tcgcgcagcc attgccggaa gtccggtacg tattgttgat attgcgctcg gtgataacga
     3181 tattgaaatc cagcgtaacc tgttgcagga gatgctggag cgccatccag aaatcgacgt
     3241 cgttgccgga acggccattg cggcagaggc ggcaatgggg gaagggcgta acctgaaaac
     3301 accgcttacc gtggtgtcgt tttatctttc acatcaggtg tatcgcgggc tgaagcgggg
     3361 aagagtgatt atggctgcca gcgatcaaat ggtctggcag ggggaactgg cggttgagca
     3421 ggccatcagg caattacagg ggcaatcggt gtctgataat gtcagcccac cgattttagt
     3481 tctgacgccg aaaaatgccg accgcgaaca tatccgccgc tcgctgtcac cggggggatt
     3541 tcgtccggtc tattattatc agcacacatc agcggctaag aaataacctt cgccatgttg
     3601 tgtcaccagt aaatccgcgc tgagtttatg gcgtaaacga cgaattaaca catcgacggt
     3661 gcgcaggtca gggttttcca cccgacgcgc agaaagcata cgcagcagac gttcacggct
     3721 gagaatttcg cccggattag tcacaaatgc caccaacatt tcatactctg cgcgggtcag
     3781 tttaatcggc tcgccatctc gctccagcgt atggcgcgac acattcaggc aataacccgc
     3841 aaagcgatag cagttgtcct gagtgtgcgg ttgcgcctgc cgcgcgaggt cgattcgcca
     3901 gagcagattt ttcactcgta ccaccagttc gcgcagttcc agcggtttgg tgacgtaatc
     3961 gtctgcgccc atttccagcc caacaatacg gtcaatccga tcgctgcgtc ccgtaaccag
     4021 aataatcccc accgttgagc gttctcgcag ggcgcgggtt aacatcaggc cattttcatc
     4081 gggcaaattg atatccagca gaattaaatc caccggctga ttctgcataa tttcccgcag
     4141 cccggcaccg ctcgccgtaa cggaaacggt atacccctcc tgagtgaagt aggattgtaa
     4201 tcgcgcctgg gtaaccggtt catcttcaac aataacaatg tgatgtggca tcagagggtt
     4261 ttactcattc tgttcatatc tgttcatatt ctgccgtaag ccgttcatcc tgaccagtgc
     4321 cgctgttcat atttgctcat taa
//
LOCUS       DAMZFT010000253         4316 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.253, whole genome shotgun sequence.
ACCESSION   DAMZFT010000253 DAMZFT010000000
VERSION     DAMZFT010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4316)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4316)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4316
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.253"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..303
                     /locus_tag="PN977_005082"
     CDS             <1..303
                     /locus_tag="PN977_005082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HDI6044998.1"
                     /translation="AYIGWAPDPWSLRVQSTTSFDVSDAQGYKVDGYTIVDLLGSYQL
                     PVGTLSFSIENLFDRDYTTVWGQRAPLYYSPGYGPASLYGYKGRGRTFGLSYSVLF"
     gene            complement(368..1584)
                     /locus_tag="PN977_005083"
                     /pseudo
     CDS             complement(368..1584)
                     /locus_tag="PN977_005083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343760.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(1902..2526)
                     /locus_tag="PN977_005084"
                     /pseudo
     CDS             complement(1902..2526)
                     /locus_tag="PN977_005084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            complement(2893..3771)
                     /gene="sitD"
                     /locus_tag="PN977_005085"
     CDS             complement(2893..3771)
                     /gene="sitD"
                     /locus_tag="PN977_005085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000968123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter permease subunit
                     SitD"
                     /protein_id="HDI6044999.1"
                     /translation="MMALLLEPLQFTFMSHALLISLVVSIPCALLSVFLVLKGWALMG
                     DAMSHAVFPGIVLAWILGFPLAGGAFVAGVFCAVATGYLKDNSRIKQDTVMGIVFSGM
                     FAAGLILYIAVKPDVHLDHILFGDMLGVTIGDIIQTVIIAGLVTLVISVKWRDFLLFS
                     FDYQQAQVSGLHTRWLHYGLLCMVSLTIVATLKAVGIILSISLLIAPGAIAVLLTQRF
                     HIALLLALGISVMVSMTGVWLSFFIDSAPAPTIVILFTVVFILTFTASSINAHAKENV
                     DPQDLPTSTTTREQHL"
     gene            complement(3768..>4316)
                     /gene="sitC"
                     /locus_tag="PN977_005086"
     CDS             complement(3768..>4316)
                     /gene="sitC"
                     /locus_tag="PN977_005086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter permease subunit
                     SitC"
                     /protein_id="HDI6045000.1"
                     /translation="LGLFMVSLNPTSVNIQTIVLGNILAIAPADILQLTIIGIVSVIV
                     LFFKWKDLMVTFFDENHARAIGLYPGRLKILFFTLLAVSTVAALQTVGAFLVICLVVT
                     PGATAWLLTDRFPRLLVIAVTIGSVTSFLGAWVSYFLDGATGGIIVVAQTLLFLLAFV
                     FAPTHGLLANRQRAHKVLEDRS"
BASE COUNT         1124 a         1090 c         1034 g         1068 t
ORIGIN      
        1 gcctacattg gctgggcacc ggacccgtgg agtctgcgag tgcagagcac cacctccttt
       61 gacgtgagcg acgcgcaggg ctacaaggtc gatggctata ccatcgtcga tttgcttggc
      121 agttatcagc ttccggtggg tacactcagc ttcagcattg aaaacctctt cgaccgtgac
      181 tacaccactg tctgggggca gcgtgcgcca ctgtactaca gcccgggtta cggccctgct
      241 tcactgtacg gctacaaagg caggggccgc acctttggtc tgagttactc agtattattc
      301 tgacaggaat tctttggaaa caaaggtatg ttgatataag ggccttccct gatcatggtg
      361 ggaaggctca aaacgccata ttcagtttcc cgtagtggaa catcacgccc agcttaaagc
      421 gttcccgatt gcggtatccc ctggctttta tcctcagcag cctgagctta ctgttaagcg
      481 cctccgcgtt tccgtttgag acacggtgtc gcatggcatt caggatccca tacagccttt
      541 ttcctatcgt tttcgtggcg tttttcatca tagtaatgtc actgttggcc gccagagcca
      601 gcaatctctc ccactcatta cgtctttcct cacttcatgg cctgttccag atattctttg
      661 ccagctcttt cagcgcccag cacaggcttg tcagcttcat ctgtgcacgc agccatatca
      721 gcttttcctg cctggattcg gtcatccatt tatcgttgta ctgccacagg aagcgggtcc
      781 cctttgcctg acgccgactt tcaacaggga ggtgtggatg ttcatcctga cggattttat
      841 caaccacttc accaagttgc tttgctacat ggaagcggtc gaaggcgatt ttctcaaccg
      901 cacagggtaa gtggatacgc gctgctctta tacagcctgc attcatgtcc attgagagcg
      961 ttttgatagc cagcaactgc ccgtcagtaa gcgtgcgaag atagccggcg aggctctctg
     1021 tgccacgatc gtccgttaag gccagtgcac gaccatcgcg atcggagatc acagttatat
     1081 aacaatgtcc ttttttaaag gcgacctcat ccacattcat atgacgggcg gataatggct
     1141 tttttatccg ggcaagacct cgcttaactg cccgggtcat aatgccgtca accgcattcc
     1201 agctgagctt aagttgtttc cttacagcat caacagtgct gattttcagc catgagagaa
     1261 cgagtcgaac agcagcgtat accgccttcc ggggccagcc cagggaacta gcaacgtctg
     1321 gcagccatgc tccgggcaca taatgcgggg cacatcggct tcgacaatag tggtgaactg
     1381 gcagatatca agatgacgcc atttacgatg acggtgatcg tgaacagaac aggatttacc
     1441 gcaggtcgga caggctaacc gggcattttt agcgattcca actgtgacgg taacagaacc
     1501 ggcgttttca tcgagagaaa gatatttaac ctgccatgga gcggtcaggt tgataatatg
     1561 agcgtagagg gacctttcgt ccatggcggt gacctttggc aattaaaaca ccatcatcat
     1621 gccttcagcc accataacaa gggaaaaccc tgatatacgg ccttaaaaag atattccgtc
     1681 tccggaacgg actgctcacc actgaataac attatgcctg ttttatgtat ctttgactga
     1741 aatttatcgc atatcctcat ttcattgccc gtatgaaaag gtaagcgaaa acctactgcc
     1801 gtagtagcca ttgagtagta ttggtaacat aggtgccaac atttcaactg atctgcgcta
     1861 agatcttggg tattctatga aaaatgtggt agtgcttcca atcagtagaa catgttcttt
     1921 tcaatgacgg aaccgatgac tttttcgttg attttcacgg aacgcgagaa gcagattgtt
     1981 ttacgtgcca gccgcttgat gcgggtgcgg aggggcaggt tgttaagttc aataggttgc
     2041 gtgaatattt taccggtcag gtatttctcc ttcgtcactt ctctgacgtt gcagcccctg
     2101 tcattgctgg tcactatgcc gatatagaac gaagtcaaca gggctaacag ttcccgacag
     2161 gtctcatcat tccgtggccc gaaagaataa gccaacacac cgcagtttta gtgttgtaag
     2221 cagaccagtg ttgttgcgcc ttgctgccaa cgaaatttaa ctgctcatca agtttcaaag
     2281 ataagggcaa catcagcgtg aacgactggc gtagatgtta tccgccgggg cgaaaacatt
     2341 tttaatgtcc gtatgacggt attgatgccg attttcagtg ttcatgggta tcacggacac
     2401 ctgcgccgtt atgtgccatt tcaacgatct gctccttaac gccgggtttc ctgacttcac
     2461 caagagtaag tgagctggaa aacatgtttt acatgagcga caacggaaac gctcatctcg
     2521 ggaacgtgtt cttaacggtt agactggccc ccctgaatca cctgcagttg aaggacagta
     2581 acaggttaag tgataacaga tgtctggaaa tatagaggta aatccaagga agtgccactt
     2641 ttcatccgtt gttgacaata cagcaaaact tgatgctatt cgtacgccca caatggaatg
     2701 tggattcaaa ctgaatattc cgtttgttga tatgagtgat ttgggcaacg gtggcaacaa
     2761 ataactttat acaggagctg acggtgtttt cctgatcatg ccgcccatat tttcttcgga
     2821 ggtcggggac tttcaatacc ttcccccgac actctatgac ttcacaagat tgtctccatg
     2881 actgctatgt gcctacagat gttgctctct ggttgttgta ctagtcggga gatcctgtgg
     2941 atcgacattt tccttcgcgt gagcattgat gctggaagcg gtaaacgtca ggatgaatac
     3001 aaccgtgaat aagataacaa tcgttggtgc tggtgcgctg tctataaaaa aggagagcca
     3061 tacaccagtc attgagacca ttaccgatat accgagcgcc agcaggagcg caatatgaaa
     3121 gcgttgcgtc aggagtaccg caatggcgcc aggcgcaatg agcagagaaa tggagagaat
     3181 gatacctacc gctttcagcg tcgccacaat ggtcagagag accatgcaca gcagcccata
     3241 atgcagccat ctcgtatgca aaccgcttac ctgcgcctgt tgataatcaa agctgaacag
     3301 caaaaaatcc cgccatttta cgctaataac aagtgtaacc agcccggcaa taatcaccgt
     3361 ctggattata tcgccgatgg ttaccccgag catgtcgccg aaaagaatat ggtcaagatg
     3421 tacgtcgggt ttgactgcaa tatacaagat cagacctgcg gcaaacatgc cagaaaaaac
     3481 aatccccatc actgtatctt gcttgattcg gctgttgtct ttcaggtatc ccgtcgccac
     3541 cgcgcagaat actccggcga cgaatgctcc tcccgccaga ggaaaaccca gtatccacgc
     3601 aagcacgata ccaggaaaaa ccgcatgact catcgcgtct cccatcagtg cccaaccttt
     3661 caggaccaga aagaccgaca gcagcgcaca gggtatggaa actaccagtg aaatcagcag
     3721 tgcgtgactc ataaaggtga actgcagtgg ctccagtaat aatgccatca tgagcgatcc
     3781 tccagcacct tatgcgcacg ctgacgattt gccaaaagcc catgggtggg tgcaaagaca
     3841 aatgctaaaa gaaacagcag tgtctgcgcc accacgatga ttccacctgt cgctccgtcc
     3901 agaaagtaac tgacccacgc gccgaggaaa ctggttacac tgccaatagt tacagcaatc
     3961 acgagcaggc ggggaaaacg atcggttaac aaccatgctg ttgcgccagg ggtcactacc
     4021 agacagatca ccagaaaagc cccaaccgtt tgcaatgctg caacggtcga taccgccagc
     4081 agtgtgaaga agaggatttt taatcttcct ggatataatc caatagcacg ggcgtgattt
     4141 tcgtcgaaaa aggtcaccat aagatctttc catttgaaaa agagcactat gactgagaca
     4201 atgccaatga tagtgagttg caggatatcc gccggagcga ttgccagaat attgcccagg
     4261 acgatggtct gaatatttac ggatgtcggg tttaatgaca ccataaacag ccccag
//
LOCUS       DAMZFT010000254         4275 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.254, whole genome shotgun sequence.
ACCESSION   DAMZFT010000254 DAMZFT010000000
VERSION     DAMZFT010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4275)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4275)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4275
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.254"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(<1..245)
                     /locus_tag="PN977_005087"
     CDS             complement(<1..245)
                     /locus_tag="PN977_005087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310565.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDI6045001.1"
                     /translation="MPKVQADGLPLPQRYGAILTIVIGISMAVLDGAIANVALPTIAT
                     DLHATPASSIWVVNAYQIAIVISLLSFSFLGDMFGYRR"
     gene            422..1213
                     /gene="kdgR"
                     /locus_tag="PN977_005088"
     CDS             422..1213
                     /gene="kdgR"
                     /locus_tag="PN977_005088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator KdgR"
                     /protein_id="HDI6045002.1"
                     /translation="MANADLDKQPDSVSSVLKVFGILQALGEEREIGITELSQRVMMS
                     KSTVYRFLQTMKTLGYVAQEGESEKYSLTLKLFELGARALQNVDLIRSADIQMREISR
                     LTKETIHLGALDEDSIVYIHKIDSMYNLRMYSRIGRRNPLYSTAIGKVLLAWRDRDEV
                     KQILEGVEYKRSTERTITSTEALLPVLDQVREQGYGEDNEEQEEGLRCIAVPVFDRFG
                     VVIAGLSISFPTLRFSEERLQEYVAMLHTAARKISAQMGYHDYPF"
     gene            complement(1357..1596)
                     /locus_tag="PN977_005089"
     CDS             complement(1357..1596)
                     /locus_tag="PN977_005089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6045003.1"
                     /translation="MRFIIRTVMLIALVWIGLLLSGYGVLIGSKENAAGLGLQCTYLT
                     ARGTSTVQYLHTKSGFLGITDCPLLRKSNIVVDNG"
     gene            1755..1898
                     /gene="mgrB"
                     /locus_tag="PN977_005090"
     CDS             1755..1898
                     /gene="mgrB"
                     /locus_tag="PN977_005090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP/PhoQ regulator MgrB"
                     /protein_id="HDI6045004.1"
                     /translation="MKKFRWVVLVVVVLACLLLWAQVFNMMCDQDVQFFSGICAINQF
                     IPW"
     gene            1973..2260
                     /locus_tag="PN977_005091"
     CDS             1973..2260
                     /locus_tag="PN977_005091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebO family protein"
                     /protein_id="HDI6045005.1"
                     /translation="MNEVVNSGVMNIASLVVSVVVLFIGLILWFFINRASSRTNEQIE
                     LLEALLDQQKRQNALLRRLCEANEPEKADKKTVESQKSVEDEDIIRLVAER"
     gene            2930..3073
                     /locus_tag="PN977_005092"
     CDS             2930..3073
                     /locus_tag="PN977_005092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YobF family protein"
                     /protein_id="HDI6045006.1"
                     /translation="MCGIFSKEVLSKHVDVEYRFSAEPYIGASCSNVSVLSMLCLRAK
                     KTI"
     gene            3086..3295
                     /gene="cspE"
                     /locus_tag="PN977_005093"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             3086..3295
                     /gene="cspE"
                     /locus_tag="PN977_005093"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HDI6045007.1"
                     /translation="MAKIKGQVKWFNESKGFGFITPADGSKDVFVHFSAIQGNGFKTL
                     AEGQNVEFEIQDGQKGPAAVNVTAI"
     gene            3461..4270
                     /gene="rlmA"
                     /locus_tag="PN977_005094"
     CDS             3461..4270
                     /gene="rlmA"
                     /locus_tag="PN977_005094"
                     /EC_number="2.1.1.187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(745)-N(1))-methyltransferase"
                     /protein_id="HDI6045008.1"
                     /translation="MSFSCPLCHQPLSREKNSYICPQRHQFDMAKEGYVNLLPVQHKR
                     SRDPGDSAEMMQARRAFLDAGHYQPLRDAIVAQLRERLDEKATAVLDIGCGEGYYTHA
                     FADALPEITTFGLDVSKVAIKAAAKRYPQVTFCVASSHRLPFSDTSMDAIIRIYAPCK
                     AEELARVVKPGGWVITATPGPRHLMELKGLIYNEVHLHAPHAEQLEGFTLQQSDELCY
                     PMRLRGDEAVALLQMTPFAWRAKSEVWQTLAAKEVFDCQTDFNIHLWQRSY"
BASE COUNT         1130 a          885 c         1053 g         1207 t
ORIGIN      
        1 cgtcgatagc caaacatatc gcccagaaac gaaaatgaga gcagggagat gacaatggcg
       61 atttgatagg cgttcactac ccagatggaa ctggctggcg tggcatgaag gtccgtggcg
      121 attgttggca gggcgacgtt ggcgattgcg ccgtcaagga cagccatcga aataccaatc
      181 acaatggtta atatcgcacc gtatcgctgg ggcaatggca ggccgtcggc ctgaactttt
      241 ggcataggaa ttttatatct ttggtgaata atcaacaggg gtatgattct agcactgata
      301 ttcacggtgg atgtcgcaga attgtaacta accggcaaaa gatggattgc gaccacccag
      361 gtgcgaaatt ataataaaaa acagttctga tttttataaa acactcgcaa tgaggtgata
      421 aatggctaac gcagatctgg ataaacagcc tgattctgta tcttctgtgc tgaaagtttt
      481 tggcattttg caggcgctgg gtgaagagcg cgaaataggg ataaccgagc tgtcgcagcg
      541 cgtcatgatg tcaaaaagca ccgtttatcg ctttttacag accatgaaaa ccctgggtta
      601 tgtggcgcag gaaggggagt cggagaaata ttcgctgacc ctgaaattgt ttgaactggg
      661 cgctcgcgcg ttgcaaaacg tcgatttaat tcgtagcgca gatatccaga tgcgtgagat
      721 ttcccgcctg acgaaagaaa ctatccacct cggcgcactg gacgaagaca gtattgttta
      781 catccacaaa attgactcta tgtacaattt gcgcatgtat tcacggattg ggcgtcgtaa
      841 tccgctgtac agcaccgcga ttggtaaggt actgctggca tggcgcgatc gcgatgaagt
      901 gaagcaaatt cttgagggcg tggagtataa acgcagtacc gagcggacca tcaccagtac
      961 agaagcgtta ttacccgttc tggaccaggt gcgcgagcag gggtatggcg aagataatga
     1021 agagcaggaa gaagggctgc gatgcattgc ggttccggta tttgatcgct ttggcgtggt
     1081 cattgccggt ttgagcatct ccttcccgac gttacgtttc tctgaagagc gtttacagga
     1141 atatgtcgca atgttgcata ccgcggcgcg caaaatttct gcccaaatgg gttatcacga
     1201 ctatccgttc tgatgagagt aagaacctgt ctgaatatca aacagacagg ttctttattt
     1261 agcatgagaa aaataaagtt gaaggtggcg ttatattaaa cgcacttgcg ataagaatat
     1321 tttactcatg agtgagaatc tggttattta ttacccttaa ccattatcga ccacaatatt
     1381 gcttttacgt aacaacgggc aatctgttat ccccagaaaa ccacttttag tgtgcaggta
     1441 ttgtaccgtg ctggtgcctc tggcggtcag ataggtacat tgcaaaccta atcccgcggc
     1501 attctctttg cttccaatca aaacgccata tccgctgagt aataatccta tccataccag
     1561 tgctatcagc ataactgtgc gaatgatgaa tcgcattaca acctcttctc tttttatgtt
     1621 cgcttaatcg tagcggcaat atgcgctgaa gcaagcgact cattctgaaa aagcacgaat
     1681 atcgacatag ttaggcgctg tttaactaac gcatgctagt ttaatgacat aaggtaggtg
     1741 aaacggagat tggagtgaaa aagtttcgat gggtcgttct ggttgtcgtg gtgttggctt
     1801 gcttgctgct ttgggcgcag gtattcaaca tgatgtgcga tcaggatgta caatttttca
     1861 gcggaatttg tgccattaac cagtttatcc cgtggtgata tcattttttc agcactagat
     1921 tttcttcctc tccagcagtg gtagaatagc gcctctactc agaggtggtg aaatgaacga
     1981 agttgtaaat tcaggcgtga tgaacattgc gtctttggtt gtatcggtgg tggttctttt
     2041 tatcgggctt atcttgtggt tttttatcaa tcgtgccagt tcacggacta acgaacagat
     2101 tgaactgctt gaggcgttgc tggatcagca aaaacgtcaa aatgctctgt tacgtcgttt
     2161 gtgcgaagca aacgaacctg agaaagcaga taaaaagacc gttgagagtc aaaaatcggt
     2221 tgaagacgaa gatattattc gtctggtcgc cgaacgataa tccttcctca atcttgagta
     2281 accccgtcgt tgaagggtta ctcaattcat cctgattata cgcactgtca tggtgcaact
     2341 gcttcaaatt aaaactctgg tctgacttca aataattatc atattattta tttgcgtaac
     2401 gggatatcag aaaaagatat ttagcccatt aatttattag gatgtttaca tcggatttgt
     2461 gattaagcgt ggtattattt attagcgaaa cgtttctctc ttgatttttt tgcgcattca
     2521 tcaatttttc ttattttaaa tttacaatac ctttggcgat tgacttctct ttggggtgat
     2581 tgatggccgt taactgactg ttttatgaga aaaagtgata taacttttta ttcattgcat
     2641 agcagaaaat gtgatattgc acgcactatg taataacttc tcccattggc atggaacaac
     2701 tgaacttatt gaactatgtt agaaaatacg tcagtttaag tatctgcctg aactggcaag
     2761 gttaagcaca atgatatatc ggcgcgtatt ccgttgcata agtgtgcaaa aaaagtggaa
     2821 gacgtatcga gatttgtgcg tctgatcgag acatgtttaa aaatggcttg ccataattaa
     2881 cgttgtatgt gataacagat ttcgggttaa acgaggtaca gttctgttta tgtgtggcat
     2941 tttcagtaaa gaagtcctga gtaaacacgt tgacgttgaa taccgcttct ctgccgagcc
     3001 ttatattggt gcctcatgca gtaatgtgtc agttttatct atgttatgcc tgcgggcgaa
     3061 gaaaacaatc taaggaattt ttcaaatggc aaagattaaa ggtcaggtta agtggttcaa
     3121 cgagtctaaa ggttttggct tcattactcc ggctgatggc agcaaagacg tgttcgtaca
     3181 cttctccgct atccagggta atggcttcaa aactctggct gaaggtcaga acgttgagtt
     3241 cgaaattcag gacggccaga aaggtccggc agctgttaac gtaacagcta tctgatcgaa
     3301 tccactgatc tgaagtgtga atacgcttca atctcgctat aaagcctcgc cgaatgcgag
     3361 gctttttact ttgctttatc ttcgctcctg gcgttcggat atttgcccgc cgcgtgattc
     3421 gcgttacact tgcggccttt agtatcctgc cggagttgtc atgtcgtttt cctgtccact
     3481 ttgccatcag cctctttcgc gtgaaaaaaa cagctatatc tgtccccagc gacatcagtt
     3541 tgatatggcg aaagaagggt atgtcaatct gctgcccgtt cagcataaac ggtctcgtga
     3601 tccgggcgac agcgcggaaa tgatgcaagc acgccgcgca ttcttagatg ccggacatta
     3661 tcagccgctg cgtgatgcaa ttgtcgccca actgcgggaa cggcttgatg aaaaggccac
     3721 ggcggtgctg gatattggct gtggtgaagg gtattacaca cacgcatttg ccgatgcgct
     3781 gcccgaaatc accacgtttg gtctggatgt ctcgaaggta gcgataaaag cggcggcgaa
     3841 acgctatccg caggtcactt tttgtgttgc ttccagccac cgtttgccgt tttccgatac
     3901 cagtatggac gccataatac gtatttacgc gccgtgtaaa gcagaagaat tagcgcgagt
     3961 agtgaagcct ggcggttggg tcattactgc cacgccggga ccgcgacatt tgatggagct
     4021 gaaggggctg atttacaatg aagtacatct tcatgcacca catgcagaac aactggaagg
     4081 ttttacatta cagcagagtg atgagttgtg ttatccgatg cgtcttcgcg gtgacgaagc
     4141 cgtcgcatta ttgcagatga cgccgtttgc ctggcgtgcg aagtcagaag tctggcaaac
     4201 actggccgca aaagaagtgt tcgactgcca gacggacttt aatattcacc tctggcagcg
     4261 ttcttattaa ccgtg
//
LOCUS       DAMZFT010000255         4268 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.255, whole genome shotgun sequence.
ACCESSION   DAMZFT010000255 DAMZFT010000000
VERSION     DAMZFT010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4268)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4268)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4268
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.255"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..1064
                     /gene="entE"
                     /locus_tag="PN977_005095"
     CDS             <1..1064
                     /gene="entE"
                     /locus_tag="PN977_005095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="(2,3-dihydroxybenzoyl)adenylate synthase EntE"
                     /protein_id="HDI6045009.1"
                     /translation="VAYFQLSGGTTGTPKLIPRTHNDYYYSVRRSVEICQFTQQTRYL
                     CAIPAAHNYAMSSPGSLGVFLAGGTVVLAADPSATLCFPLIEKHQVNVTALVPPAVSL
                     WLQALAEGESRAQLASLKLLQVGGARLSATLAARIPAEIGCQLQQVFGMAEGLVNYTR
                     LDDSAEKIIHTQGYPMCPDDEVWVADAEGNPLPQGEVGRLMTRGPYTFRGYYKSPQHN
                     ASAFDANGFYCSGDLISIDPEGYITVQGREKDQINRGGEKIAAEEIENLLLRHPAVIY
                     AALVSMEDELMGEKSCAYLVVKEPLRAVQVRRFLREQGIAEFKLPDRVECVDSLPLTA
                     VGKVDKKQLRQWLASRTSA"
     gene            1078..1935
                     /gene="entB"
                     /locus_tag="PN977_005096"
     CDS             1078..1935
                     /gene="entB"
                     /locus_tag="PN977_005096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin biosynthesis bifunctional
                     isochorismatase/aryl carrier protein EntB"
                     /protein_id="HDI6045010.1"
                     /translation="MAIPKLQAYALPDSHDIPQNKVDWAFEPQRAALLIHDMQDYFVS
                     FWGENCPMMEQVIANIAALRDYCKQHNIPVYYTAQPKEQSDEDRALLNDMWGPGLTRS
                     PEQQKVVDRLTPDADDTVLVKWRYSAFHRSPLEQMLKESGRNQLIITGVYAHIGCMTT
                     ATDAFMRDIKPFMVADALADFSRDEHLMSLKYVAGRSGRVVMTEELLPAPIPASKAAL
                     REVILPLLDESDEPFDDDNLIDYGLDSVRMMALAARWRKVHGDIDFVMLAKNPTIDAW
                     WKLLSREVK"
     gene            1935..2681
                     /gene="entA"
                     /locus_tag="PN977_005097"
     CDS             1935..2681
                     /gene="entA"
                     /locus_tag="PN977_005097"
                     /EC_number="1.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase
                     EntA"
                     /protein_id="HDI6045011.1"
                     /translation="MDFSGKNVWVTGAGKGIGYATALAFVEAGAKVTGFDQAFAQEQY
                     PFATEVMDVADAAQVAQVCQRLLAQTERLDVLVNAAGILRMGATDQLSKEDWQQTFAV
                     NVGGAFNLFQQTMNQFRRQRGGAIVTVASDAAHTPRIGMSAYGASKAALKSLALSVGL
                     ELAGSGVRCNVVSPGSTDTDMQRTLWVSDDAEEQRIRGFGEQFKLGIPLGKIARPQEI
                     ANTILFLASDLASHITLQDIVVDGGSTLGA"
     gene            2684..3097
                     /gene="entH"
                     /locus_tag="PN977_005098"
     CDS             2684..3097
                     /gene="entH"
                     /locus_tag="PN977_005098"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proofreading thioesterase EntH"
                     /protein_id="HDI6045012.1"
                     /translation="MIWKRHLTLDELNATSDNTMVAHLGIVYTRLGDDVLEAEMPVDT
                     RTHQPFGLLHGGASAALAETLGSMAGFMMTRDGQCVVGTELNATHHRPVSEGKVRGVC
                     QPLHLGRQNQSWEIVVFDEQGRRCCTCRLGTAVLG"
     gene            3278..>4268
                     /locus_tag="PN977_005099"
     CDS             3278..>4268
                     /locus_tag="PN977_005099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation protein A"
                     /protein_id="HDI6045013.1"
                     /translation="MNKSGKYLVWTVLSIMGAFALGYIALNRGEQINALWIVVASVCI
                     YLIAYRFYGLYIAKNVLAVDPTRMTPAVRHNDGLDYVPTDKKVLFGHHFAAIAGAGPL
                     VGPVLAAQMGYLPGMIWLLAGVVLAGAVQDFMVLFVSTRRDGRSLGELVKEEMGPTAG
                     VIALVACFMIMVIILAVLAMIVVKALTHSPWGTYTVAFTIPLALFMGIYLRYLRPGRI
                     GEVSVIGLVFLIFAIISGGWVAESPTWAPYFDFTGVQLTWMLVGYGFVAAVLPVWLLL
                     APRDYLSTFLKIGTIVGLAVGILIMRPTLTMPALTKFVDGTGPVWTGNLFPFLF"
BASE COUNT          896 a         1071 c         1320 g          981 t
ORIGIN      
        1 aagtggccta tttccagctt tccggcggca ccaccggcac tccgaaactg atcccgcgca
       61 ctcataacga ctactactac agcgtgcgtc gtagcgtcga gatttgtcag ttcacacaac
      121 agacgcgcta cctgtgcgcg atcccggcgg ctcataacta cgccatgagt tcaccgggat
      181 cgctgggcgt ctttctcgct ggcggcactg tcgttctggc tgccgacccc agcgccacgc
      241 tttgcttccc attgattgaa aaacatcagg tgaacgtcac cgcgctggtg ccgccagcag
      301 tcagcctgtg gttgcaggca ctggctgaag gcgaaagccg ggcgcagctt gcctcgctga
      361 aactgttaca ggtcggcggc gcacgtcttt ctgccacgct tgcggcgcgt attcccgctg
      421 agattggctg ccagttgcag caggtgtttg gcatggcgga agggctggtg aactacaccc
      481 gtcttgatga tagtgcggag aaaattatcc atacccaggg ttacccaatg tgcccggacg
      541 atgaagtatg ggttgccgat gccgaaggaa atccactgcc gcaaggggaa gttggacgcc
      601 tgatgacgcg cgggccgtac accttccgtg gctattacaa aagcccgcag cacaatgcca
      661 gcgcctttga tgccaacggt ttttactgtt ccggcgatct gatctctatt gatccagagg
      721 gttacatcac cgtgcagggg cgcgagaaag atcagatcaa ccgtggcggc gagaagatcg
      781 ctgccgaaga gatcgaaaac ctgctgctgc gccacccggc ggtgatctac gccgcactgg
      841 tgagcatgga agatgagctg atgggcgaaa aaagctgtgc ttatctggtg gtaaaagagc
      901 cgctgcgcgc ggtgcaggtg cgtcgtttcc tgcgtgaaca gggtattgcc gaatttaaat
      961 taccggatcg cgtggagtgt gtggattcac ttccgctgac ggcggtcggg aaagtcgata
     1021 aaaaacaatt acgtcagtgg ctggcgtcac gcacatcagc ctgaaggaga gaacacgatg
     1081 gctattccaa aattacaggc ttacgcactg ccggattctc acgatattcc gcagaataaa
     1141 gtcgactggg cctttgaacc gcaacgtgcc gcgttgttaa tccatgatat gcaggactat
     1201 tttgtcagct tctggggtga gaactgcccg atgatggagc aggtgatcgc gaatattgcg
     1261 gcactacgcg actactgcaa acagcacaat atcccggttt attacaccgc ccagccgaaa
     1321 gagcagagcg atgaagatcg ggcgctgttg aatgatatgt gggggccagg cctgacccgt
     1381 tcgccggaac agcaaaaggt ggtggatcgc ctgacgccag atgctgacga cacggtgttg
     1441 gtgaagtggc gctacagcgc gtttcatcgt tccccgctgg agcaaatgct gaaagagagt
     1501 ggacgtaacc agctgattat taccggggta tatgcccaca ttggctgtat gaccaccgca
     1561 acggacgcat ttatgcgcga tattaaaccg tttatggtgg cggatgcgct ggccgatttc
     1621 agccgtgacg agcatttgat gtcgctgaaa tatgtggccg gacgttctgg tcgggtggtg
     1681 atgaccgaag aattgctgcc agcacctatc cccgccagca aagcggcgct gcgtgaggtg
     1741 atcctgccgt tgctggacga gtccgatgaa ccgttcgatg acgacaacct gatcgactac
     1801 ggtctggatt cggtgcggat gatggcgctg gcggcgcgct ggcgcaaagt gcatggcgat
     1861 atcgactttg tcatgctggc gaaaaacccg accatcgacg cctggtggaa gctactttcc
     1921 cgcgaggtga aataatggat ttcagcggta aaaatgtctg ggtaactggc gcagggaaag
     1981 gtatcggcta cgccacggcg ctggcgtttg ttgaggcggg agcgaaagtc accggttttg
     2041 atcaagcgtt cgctcaggag caatatccct ttgcgaccga agtgatggat gttgccgatg
     2101 ctgcgcaggt ggcgcaggta tgtcagcgac tgttagcgca aacggagcga ctggacgtgc
     2161 tggtcaatgc ggcgggaatt ttgcgcatgg gcgcgaccga tcagctcagt aaagaggact
     2221 ggcagcagac ttttgcggtc aatgtcggcg gtgcgtttaa cctgttccag cagaccatga
     2281 accagtttcg ccgtcagcgg ggcggggcga ttgttactgt tgcgtccgac gccgcacaca
     2341 cgccgcgcat tggtatgagt gcttatggcg cgtcgaaagc ggcgctgaaa agccttgcgt
     2401 tgagcgttgg gctggaactg gcgggtagcg gggtgcgctg taatgtggtt tcgccgggct
     2461 ccaccgacac cgatatgcaa cgcacgctgt gggtgagcga tgacgccgaa gagcagcgta
     2521 ttcgcggctt tggcgagcag tttaaactcg gaattccgct agggaaaatc gcccgtccgc
     2581 aggagatcgc caatacgatt ttgttcctcg cctctgacct cgccagccat atcaccctac
     2641 aggatattgt ggtcgatggc ggctcaacgc tgggggcata agcatgatct ggaaacgcca
     2701 tttaacgctc gacgagctga acgccaccag cgataacaca atggtggcgc atctgggaat
     2761 tgtgtatacc cgtctgggcg atgatgtact cgaagccgaa atgccggttg atacccgtac
     2821 tcatcagccg tttggcctgc tgcatggcgg cgcgtcggcg gcgctggcgg aaacgctggg
     2881 atcgatggcc ggatttatga tgacccgcga cggacagtgt gtggtgggca cggaacttaa
     2941 cgccacacat catcgcccgg tgtctgaggg aaaggtgcgc ggtgtctgcc agccgctgca
     3001 tcttggacga caaaatcaga gctgggaaat cgtcgttttc gatgaacagg ggcggcgttg
     3061 ctgcacctgt cggctgggca cagcggtttt gggatgaacc cgacagaata aaatgagatt
     3121 gcaggaaaat tcggttaacg gagtgatcga gttaacgttg ttaagttaaa tattggtttt
     3181 aactctgatt tatatggttg ctgtgttgtt aaattgtaca aagatgttat agaaacaaaa
     3241 tgtaacatct ctatggacac gcacacggat aacaactatg aacaaatcag ggaaatacct
     3301 cgtctggaca gtgctctcta taatgggagc atttgctctg ggatacattg ctttaaatcg
     3361 tggggaacag atcaacgcgc tgtggattgt agtggcgtcg gtctgtatct atctgatcgc
     3421 ttaccgtttt tatggtcttt atatcgccaa aaatgtattg gcggttgatc cgacgcgtat
     3481 gacgccagcg gtgcgccata acgacgggct ggactatgtg ccgacggaca agaaagtgct
     3541 gttcggtcac cattttgcgg ccattgccgg agctggcccg ctggtggggc cggtactggc
     3601 ggcgcaaatg ggttacctgc cagggatgat ctggctgctc gccggggtgg ttctcgccgg
     3661 tgcggtgcag gatttcatgg tgctgtttgt ttctacgcgc cgtgacggtc gctcgctggg
     3721 tgagctggtc aaagaagaga tggggccaac cgccggggtg atagcgctgg tggcctgctt
     3781 tatgatcatg gtcattatcc ttgccgtgct ggcgatgatc gtggtgaaag ccctgactca
     3841 tagcccgtgg ggaacgtata ccgttgcgtt caccattccg ctggcgctgt ttatggggat
     3901 ctacctgcgc tatttgcgtc cggggcgtat tggggaagtg tcggtgattg gcctggtatt
     3961 cctgattttc gccattatct ctggcggctg ggtggcagaa agcccgacct gggcaccgta
     4021 ctttgacttt accggcgtgc agctgacctg gatgctggtg ggttacggct tcgtggcggc
     4081 ggtgctgccg gtgtggttgc tgctggcccc gcgtgactac ctctcaacct tcctgaaaat
     4141 cgggactatc gtcggtctgg cggtaggcat tctgattatg cgcccgacgc tgaccatgcc
     4201 tgcgctaact aaatttgtcg atggcactgg cccggtatgg accggtaacc tgttcccgtt
     4261 cctgttta
//
LOCUS       DAMZFT010000256         4205 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.256, whole genome shotgun sequence.
ACCESSION   DAMZFT010000256 DAMZFT010000000
VERSION     DAMZFT010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4205)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4205)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4205
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.256"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(<1..165)
                     /locus_tag="PN977_005100"
     CDS             complement(<1..165)
                     /locus_tag="PN977_005100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HDI6045014.1"
                     /translation="MTNVLIVEDEQAIRRFLRTALEGDGMRVFEAETLQRGLLEAATR
                     KPDLIILDLGL"
     gene            complement(162..2846)
                     /gene="kdpD"
                     /locus_tag="PN977_005101"
     CDS             complement(162..2846)
                     /gene="kdpD"
                     /locus_tag="PN977_005101"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     KdpD"
                     /protein_id="HDI6045015.1"
                     /translation="MNNEPLRPDPDRLLEQTAAPHRGKLKVFFGACAGVGKTWAMLAE
                     AQRLRAQGLDIVVGVVETHGRKDTAAMLEGLAVLPLKRQAYRGRHISEFDLDAALARR
                     PALILMDELAHSNAPGSRHPKRWQDIEELLEAGIDVFTTVNVQHLESLNDVVSGVTGI
                     QVRETVPDPFFDAADDVVLVDLPPDDLRQRLKEGKVYIAGQAERAIEHFFRKGNLIAL
                     RELALRRTADRVDEQMRAWRGHPGEEKVWHTRDAILLCIGHNTGSEKLVRAAARLASR
                     LGSVWHAVYVETPALHRLPEKKRRAILSALRLAQELGAETATLSDPAEEKAVVRYARE
                     HNLGKIILGRPASRRWWRRETFADRLARIAPDLDQVLVALDEPPARTINNALDSRSFK
                     DKWRVQIQGCVVAAALCAVITLIAMQWLMAFDAANLVMLYLLGVVMVALFYGRWPSVV
                     ATVINVVSFDLFFIAPRGTLAVSDVQYLLTFAVMLTVGLVIGNLTAGVRYQARVARYR
                     EQRTRHLYEMSKALAVGRSPQDIAATSEQFIASTFHARSQVLLPDDNGKLQPLTHPQG
                     MTPWDDAIAQWSFDKGLPAGAGTDTLPGVPYQILPLKSGEKTYGLVVVEPGNLRQLMI
                     PEQQRLLETFTLLVANALERLTLTASEEQARMASEREQIRNALLAALSHDLRTPLTVL
                     FGQAEILTLDLASEGSPHARQASEIRQHVLNTTRLVNNLLDMARIQSGGFNLKKEWLT
                     LEEVVGSALQMLEPGLSSPINLSLPEPLTLIHVDGPLFERVLINLLENAVKYAGAQAE
                     IGIDAHVEGENLQLDVWDNGPGLPPGQEQTIFDKFARGNKESAVPGVGLGLAICRAIV
                     DVHGGTITAFNRPEGGACFRVTLPQQTAPELEEFHEDM"
     gene            complement(2839..3411)
                     /gene="kdpC"
                     /locus_tag="PN977_005102"
     CDS             complement(2839..3411)
                     /gene="kdpC"
                     /locus_tag="PN977_005102"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit C"
                     /protein_id="HDI6045016.1"
                     /translation="MSGLRPALSTFLFLLLITGGVYPLLTTALGQWWFPWQANGSLIR
                     EGDTVRGSALIGQNFTGNGYFHGRPSATAEMPYNPQASGGSNLAVSNPELDKLIAARV
                     AALRAANPDASASVPVELVTASASGLDNNITPQAAAWQIPRVAKARNLSVEQLTQLIA
                     KYSQQPLVNYIGQPVVNIVELNLALDKLDE"
     gene            complement(3420..>4205)
                     /locus_tag="PN977_005103"
     CDS             complement(3420..>4205)
                     /locus_tag="PN977_005103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HDI6045017.1"
                     /translation="QVARQGATPLVVVEGSRVLGVIALKDIVKGGIKERFAQLRKMGI
                     KTVMITGDNRLTAAAIAAEAGVDDFLAEATPEAKLALIRQYQAEGRLVAMTGDGTNDA
                     PALAQADVAVAMNSGTQAAKEAGNMVDLDSNPTKLIEVVHIGKQMLMTRGSLTTFSIA
                     NDVAKYFAIIPAAFAATYPQLNALNIMRLHSPDSAILSAVIFNALIIVFLIPLALKGA
                     SYKPLTASAMLRRNLWIYGLGGLLVPFIGIKVIDLLLTVCGLV"
BASE COUNT          929 a         1270 c         1118 g          888 t
ORIGIN      
        1 caggccgaga tcgagaataa tcaaatctgg cttacgggtt gccgcttcca gcaagccgcg
       61 ttgcagcgtt tcggcctcaa agacgcgcat cccgtcgccc tccagcgccg tgcgcagaaa
      121 gcgacgaata gcctgttcat cttcaacaat cagaacgttt gtcacatatc ctcatgaaat
      181 tcttcaagtt caggggcagt ttgctgggga agtgtaacac gaaaacaggc accaccttcc
      241 ggtcggttga acgcggtaat ggtgccgccg tgtacatcca caatcgcccg acaaattgcc
      301 agcccaagcc ctacccccgg caccgccgac tctttatttc cacgagcaaa cttatcgaaa
      361 atcgtctgct cctggcctgg tggaagaccg gggccattat cccagacatc cagttgtaga
      421 ttttcgccct caacgtgggc atcgatacca atttcggcct gcgcacccgc gtatttcacc
      481 gcgttctcca gcagattaat cagcacccgt tcaaacagtg gcccgtcaac gtggattaag
      541 gtcagcggtt ctggcagaga aagattgatg ggcgacgata aacccggttc cagcatttgc
      601 agcgcgctgc cgaccacttc ttccagcgtt aaccactctt tcttcaaatt aaagccgccg
      661 gactgaattc gcgccatatc cagtagatta ttcaccagtc gggtcgtgtt cagcacatgc
      721 tggcgaatct cgctggcctg gcgggcgtgg ggcgatcctt cgcttgccag atcgagcgtt
      781 aagatttctg cctgaccgaa cagcaccgta agcggcgtgc gtaaatcatg cgaaagtgcc
      841 gccagcaggg cgttgcggat ctgttctcgt tcgcttgcca tccgcgcctg ttcttcgctg
      901 gcggtcagcg tcagccgctc aagggcattg gcgactaaca gcgtaaacgt ctccagcagg
      961 cgctgctgtt ccgggatcat caattgacgc agattccccg gttccaccac caccagtccg
     1021 taggttttct cgccgctttt tagcggcaaa atctggtacg gtacaccggg taacgtgtcg
     1081 gtgcccgcgc ccgcaggcag gcctttatca aaactccact gcgcgatggc atcgtcccac
     1141 ggcgtcattc cttgcggatg tgttaacggc tgcaatttac cgttgtcatc gggtaacagc
     1201 acctgactgc gggcatgaaa cgtggaggca ataaattgtt cgctggtggc ggcgatgtcc
     1261 tgcggactgc ggcccacagc cagagctttc gacatttcat ataagtgccg tgtgcgttgc
     1321 tcgcggtaac gggctacccg cgcctgataa cgcacgcccg cagtaaggtt cccgatcacc
     1381 agcccgacgg ttaacatcac cgcgaaggtc agtagatatt gcacatcaga gacggcgagc
     1441 gtgccgcgtg gggcgataaa aaagagatcg aaactcacca cattaatgac ggtggcaacc
     1501 actgaaggcc agcgtccata aaatagcgcc accatcacca cgccaagcag atacagcatc
     1561 accaggttgg cggcatcaaa cgccatcagc cactgcatgg caattaaggt gataacggcg
     1621 cataacgcgg cggcaaccac gcatccctga atctgtaccc gccacttgtc tttaaaagag
     1681 cggctatcca gcgcgttgtt tatcgtgcgg gcgggtggtt catcaagcgc gaccagcacc
     1741 tgatcgagat cgggggcgat tcgcgccagt cggtcagcaa acgtttcccg acgccaccag
     1801 cggcgcgagg ctgggcgacc gagaataatc ttgccgagat tatgttcacg ggcataacgc
     1861 actaccgctt tctcttccgc tggatcagaa agtgttgccg tctctgcgcc cagctcctgc
     1921 gccagacgta aggcgctgag aattgcccga cgttttttct ccggtaagcg gtgcagggca
     1981 ggggtttcaa catacaccgc gtgccagacg cttcccagcc gtgacgccag ccgcgccgcc
     2041 gcgcggacca gtttttcgct gccggtgtta tgcccgatgc ataaaaggat cgcgtcgcgc
     2101 gtgtgccaga ctttctcttc gccaggatgc ccccgccagg cgcgcatttg ctcatcaacg
     2161 cgatcggcag tacggcgcag tgccagttcg cgcagggcga tcagattacc tttacgaaaa
     2221 aaatgttcaa tggcgcgctc cgcctgcccg gcaatataga ctttgccttc tttcagtcgc
     2281 tggcgcagat cgtccggggg caagtccacc agtactacgt cgtcggcggc atcaaaaaaa
     2341 ggatcgggca cggtttcccg tacctgaatt ccggtgacgc cgctgaccac atcattcaga
     2401 ctttccagat gctgaacgtt gacggtagtg aaaacatcaa tgccagcttc cagcagttct
     2461 tcaatatcct gccagcgttt gggatgacga gaacctggcg cattactgtg cgccagttcg
     2521 tccattaaga tcagtgccgg acggcgggcg agggcggcat cgagatcaaa ctcgctgatg
     2581 tgccgcccac ggtacgcctg gcgttttaac ggcagaacag ccagcccttc cagcattgcg
     2641 gcggtatctt tccgcccgtg ggtttctacc acgccaacca caatatccag cccttgcgcc
     2701 cgcagtcgct gggcttctgc cagcatcgcc caggtcttcc cgacgcctgc gcaggcaccg
     2761 aagaaaactt tcagcttccc ccgatgcggc gcggcagttt gttccagcag acgatcgggg
     2821 tcgggacgta agggttcgtt attcatcaag cttatccagc gccagattga gttcgacaat
     2881 gttgacgacc ggctggccga tatagttcac cagcggttgt tggctgtatt ttgcgatcag
     2941 ttgcgtgagc tgttcaacgc tgagattacg cgctttcgcc acgcgtggga tctgccatgc
     3001 cgccgcttgc ggggtgatat tattgtccag cccgcttgcc gatgccgtca ccagctcaac
     3061 cggaacgctc gcgctggcat ccgggttagc ggcccgtaat gcagcaacgc gtgcggctat
     3121 tagtttatcc agctcagggt tactgaccgc cagattgctc ccgccagaag cctgtggatt
     3181 atagggcatt tctgccgttg ccgacgggcg accatgaaaa tagccgttgc cagtaaaatt
     3241 ctgcccgatc aatgccgaac cgcgcaccgt atcaccttca cgaatcaacg aaccgttggc
     3301 ctgccaggga aaccaccatt gccccagcgc ggtggtcagc agcgggtaaa cgccgccagt
     3361 aatcaataac agaaagagaa atgtcgataa tgccggacgt aatccactca tggtaaacct
     3421 cacaccagac cgcaaacggt tagcagtaaa tcaatgactt tgataccaat aaacggcacc
     3481 agcagcccac ccagaccgta aatccataag ttacggcgca acatggcaga agcggtaagc
     3541 ggtttataac tcgcgccttt taacgccagg ggaatcaaaa agacgataat caaggcgttg
     3601 aaaatcaccg cactgagaat ggcggagtcg ggcgaatgca ggcgcatgat gttcagcgca
     3661 tttaactgcg gatacgttgc cgcgaacgcc gccggaataa tggcgaagta tttcgccaca
     3721 tcgttggcaa tgctgaaggt ggtcagcgag ccacgggtca tcagcatctg tttgccaata
     3781 tgtaccacct cgatcaactt ggtcgggttg gagtcgagat ccaccatatt gcccgcttct
     3841 ttcgccgcct gagtaccaga gttcatcgcc accgcgacat ctgcctgcgc cagcgccgga
     3901 gcatcgttgg tgccgtcgcc ggtcatcgcc accaaacggc cttccgcctg atactgacga
     3961 atcaatgcca gcttggcctc cggtgtcgct tcggcgagaa aatcatcgac acccgcttcc
     4021 gcagctatcg cggcggcagt cagacggtta tcaccggtaa tcatcaccgt tttaatgccc
     4081 attttgcgca gttgggcgaa gcgctcttta ataccgcctt tgacgatatc tttcagcgca
     4141 ataacgccca gcacacgaga accttccacc accaccagcg gcgtggctcc ctgacgcgca
     4201 acctg
//
LOCUS       DAMZFT010000257         4153 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.257, whole genome shotgun sequence.
ACCESSION   DAMZFT010000257 DAMZFT010000000
VERSION     DAMZFT010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4153)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4153)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4153
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.257"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            244..837
                     /gene="tetC"
                     /locus_tag="PN977_005104"
     CDS             244..837
                     /gene="tetC"
                     /locus_tag="PN977_005104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000428546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracyline resistance-associated
                     transcriptional repressor TetC"
                     /protein_id="HDI6045018.1"
                     /translation="MENKNHQQENFKSTYQSLVNSARILFVEKGYQAVSIDEISGKAL
                     VTKGAFYHHFKNKKQLLSACYKQQLIMIDAYITTKTDLTNGWSALESIFEHYLDYIID
                     NNKNLIPIQEVMPIIGWNELEKISLEYITGKVNAIVSKLIQENQLKAYDDDVLKNLLN
                     GWFMHIAIHAKNLKELADKKGQFIAIYRGFLLSLKDK"
     gene            complement(950..2155)
                     /gene="tet(B)"
                     /locus_tag="PN977_005105"
     CDS             complement(950..2155)
                     /gene="tet(B)"
                     /locus_tag="PN977_005105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001089064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(B)"
                     /protein_id="HDI6045019.1"
                     /translation="MNSSTKIALVITLLDAMGIGLIMPVLPTLLREFIASEDIANHFG
                     VLLALYALMQVIFAPWLGKMSDRFGRRPVLLLSLIGASLDYLLLAFSSALWMLYLGRL
                     LSGITGATGAVAASVIADTTSASQRVKWFGWLGASFGLGLIAGPIIGGFAGEISPHSP
                     FFIAALLNIVAFLVVMFWFRETKNTRDNTDTEVGVETQSNSVYITLFKTMPILLIIYF
                     SAQLIGQIPATVWVLFTENRFGWNSMMVGFSLAGLGLLHSVFQAFVAGRIATKWGEKT
                     AVLLGFIADSSAFAFLAFISEGWLVFPVLILLAGGGIALPALQGVMSIQTKSHQQGAL
                     QGLLVSLTNATGVIGPLLFAVIYNHSLPIWDGWIWIIGLAFYCIIILLSMTFMLTPQA
                     QGSKQETSA"
     gene            2237..2860
                     /gene="tetR(B)"
                     /locus_tag="PN977_005106"
     CDS             2237..2860
                     /gene="tetR(B)"
                     /locus_tag="PN977_005106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000088605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance transcriptional
                     repressor TetR(B)"
                     /protein_id="HDI6045020.1"
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWH
                     VKNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLG
                     TRPTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERET
                     PTTDSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     gene            complement(2838..3524)
                     /locus_tag="PN977_005107"
     CDS             complement(2838..3524)
                     /locus_tag="PN977_005107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HDI6045021.1"
                     /translation="MANLIRKEVTFESSIAAIGAAMSDISRVKILSALMDGRAWTATE
                     LSSVANISASTASSHLSKLLDCQLITVVAQGKHRYFRLAGKDIAELMESMMGISLNHG
                     VHAKVSTPVHLRKARTCYDHLAGEVAVKIYDSLCQQQWITENGSMITLSGIQYFHEMG
                     IDVPSKHSRKICCACLDWSERRFHLGGYVGAALFSLYESKGWLTRHLGYREVTITEKG
                     YAAFKTHFHI"
     gene            complement(3532..3918)
                     /locus_tag="PN977_005108"
     CDS             complement(3532..3918)
                     /locus_tag="PN977_005108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000460649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid-binding protein"
                     /protein_id="HDI6045022.1"
                     /translation="MFDVHVVLDNQIGQLALLGKTLGNKGIGLEGGGIFTVGDECHAH
                     FLVEQGKEAKIALEQAGLLVLAIRTPLIRKLKQEKPGELGEIARVLAENNINILVQYS
                     DHANQLILITDNDSMAASVTLPWAIK"
     gene            complement(3911..4126)
                     /locus_tag="PN977_005109"
     CDS             complement(3911..4126)
                     /locus_tag="PN977_005109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001384468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HDI6045023.1"
                     /translation="MSIERFQSLATEGKMLSLSWWENEYAVLQWKNHVLHAKAQQEGR
                     ESIFDFYKISIAHITREYSFKKDKDNV"
BASE COUNT         1368 a          874 c          743 g         1168 t
ORIGIN      
        1 attcgccaca tcatcaagca gtaaagactg atctaaatta tgttcaatcc ataatagaac
       61 atctttgata accgttgtat gaaactgctt atcatcatat cttaattgga tgttattagc
      121 tttattttga tagcgagaat gctgttcaat atacataaaa taacctaaat gttcttaaga
      181 ttgtcacgac cacatcatca tgataccata aacatactga cggtatgtta ttttaaatct
      241 atcatggaaa ataaaaatca tcaacaagaa aattttaaga gtacctatca atcactggtt
      301 aactcagcac gaatattgtt tgttgaaaaa ggctatcaag ctgtttcaat agatgagatc
      361 tcgggaaaag cgttggtgac caaaggtgcc ttttatcatc actttaaaaa taaaaaacaa
      421 ttactcagtg cctgttataa gcagcaatta attatgattg atgcctacat cacaacaaaa
      481 actgatttaa caaatggttg gtctgcctta gaaagtatat ttgaacatta tcttgattat
      541 attattgata ataataaaaa ccttatccct atccaagaag tgatgcctat cattggttgg
      601 aatgaacttg aaaaaattag ccttgaatac attactggta aggtaaacgc cattgtcagc
      661 aaattgatcc aagagaacca acttaaagct tatgatgatg atgtgcttaa aaacttactc
      721 aatggctggt ttatgcatat cgcaatacat gcgaaaaacc taaaagagct tgccgataaa
      781 aaaggccaat ttattgctat ttaccgcggc tttttattga gcttgaaaga taaataaaat
      841 agataggttt tatttgaagc taaatcttct ttatcgtaaa aaatgccctc ttgggttatc
      901 aagagggtca ttatatttcg cggaataaca tcatttggtg acgaaataac taagcacttg
      961 tctcctgttt actcccctga gcttgagggg ttaacatgaa ggtcatcgat agcaggataa
     1021 taatacagta aaacgctaaa ccaataatcc aaatccagcc atcccaaatt ggtagtgaat
     1081 gattataaat aacagcaaac agtaatgggc caataacacc ggttgcattg gtaaggctca
     1141 ccaataatcc ctgtaaagca ccttgctgat gactctttgt ttggatagac atcactccct
     1201 gtaatgcagg taaagcgatc ccaccaccag ccaataaaat taaaacaggg aaaactaacc
     1261 aaccttcaga tataaacgct aaaaaggcaa atgcactact atctgcaata aatccgagca
     1321 gtactgccgt tttttcgccc catttagtgg ctattcttcc tgccacaaag gcttggaata
     1381 ctgagtgtaa aagaccaaga cccgctaatg aaaagccaac catcatgcta ttccatccaa
     1441 aacgattttc ggtaaatagc acccacaccg ttgcgggaat ttggcctatc aattgcgctg
     1501 aaaaataaat aatcaacaaa atgggcatcg ttttaaataa agtgatgtat accgaattcg
     1561 attgcgtctc aacccctact tcggtatctg tattatcacg tgtatttttg gtttcacgga
     1621 accaaaacat aaccacaagg aaagcgacaa tatttagcaa cgcagcgata aaaaagggac
     1681 tatgcggtga aatctctcct gcaaaaccac caataatagg ccccgctatt aaaccaagcc
     1741 caaaacttgc ccctaaccaa ccgaaccact tcacgcgttg agaagctgag gtggtatcgg
     1801 caatgaccga tgccgcgaca gccccagtag ctcctgtgat ccctgaaagc aaacggccta
     1861 aatacagcat ccaaagcgca cttgaaaaag ccagcaataa gtaatccagc gatgcgccta
     1921 ttaatgacaa caacagcact gggcgccgac caaatcggtc agacattttt ccaagccaag
     1981 gagcaaagat aacctgcatt aacgcataaa gtgcaagcaa tacgccaaag tggttagcga
     2041 tatcttccga agcaataaat tcacgtaata acgttggcaa gactggcatg ataaggccaa
     2101 tccccatggc atcgagtaac gtaattacca atgcgatctt tgtcgaacta ttcatttcac
     2161 ttttctctat cactgatagg gagtggtaaa ataactctat caatgataga gtgtcaacaa
     2221 aaattaggaa ttaatgatgt ctagattaga taaaagtaaa gtgattaaca gcgcattaga
     2281 gctgcttaat gaggtcggaa tcgaaggttt aacaacccgt aaactcgccc agaagctagg
     2341 tgtagagcag cctacattgt attggcatgt aaaaaataag cgggctttgc tcgacgcctt
     2401 agccattgag atgttagata ggcaccatac tcacttttgc cctttagaag gggaaagctg
     2461 gcaagatttt ttacgtaata acgctaaaag ttttagatgt gctttactaa gtcatcgcga
     2521 tggagcaaaa gtacatttag gtacacggcc tacagaaaaa cagtatgaaa ctctcgaaaa
     2581 tcaattagcc tttttatgcc aacaaggttt ttcactagag aatgcattat atgcactcag
     2641 cgctgtgggg cattttactt taggttgcgt attggaagat caagagcatc aagtcgctaa
     2701 agaagaaagg gaaacaccta ctactgatag tatgccgcca ttattacgac aagctatcga
     2761 attatttgat caccaaggtg cagagccagc cttcttattc ggccttgaat tgatcatatg
     2821 cggattagaa aaacaactta aatgtgaaag tgggtcttaa aagcagcata acctttttcc
     2881 gtgatggtaa cttcacggta accaagatgt cgagttaacc accctttaga ttcataaagc
     2941 gaaaataatg cggctccaac gtacccacct aaatggaaac ggcgttcact ccaatctaaa
     3001 cacgcacaac agattttacg tgaatgtttg gaaggaacgt caattcccat ttcatgaaaa
     3061 tattgaatac cacttaatgt gatcattgaa ccattttcag tgatccattg ctgttgacaa
     3121 agggaatcat agatcttaac ggcaacttcg ccagctaaat gatcatagca agtacgtgct
     3181 tttcgtaaat gcactggcgt ggaaactttg gcatgtacgc catggtttaa ggagatcccc
     3241 atcatacttt ccatcaattc agcaatatct tttcctgcta gccgaaaata acgatgcttg
     3301 ccttgagcta ctactgtgat tagctggcaa tctaataatt tagataaatg actgctcgcc
     3361 gttgaagctg atatattcgc cacagaactt agctcagtgg ccgtccaagc tcgcccatcc
     3421 atcaaagcac tgagtatttt aactcgtgaa atgtcagaca tagccgcccc tatcgcggct
     3481 attgaggact caaaggtaac ctcttttcgt attaaattag ccatcgcaag ttcactttat
     3541 tgcccaaggg agcgtaacag atgcagccat actatcattg tccgttatta atatcagttg
     3601 gttagcatgg tcactgtatt gcactaaaat attaatgtta ttctccgcca atactcgtgc
     3661 tatttcgcca agttcccccg gtttttcctg ttttaactta cgaattaatg gtgtccggat
     3721 cgcaagtact aacagtccag cttgctctag cgctatttta gcttcctttc cttgttcaac
     3781 aagaaaatga gcatggcatt catcaccaac cgtaaatatc cctccccctt ccaatccaat
     3841 acctttatta cctaatgttt ttcctagtaa tgctaattgt cctatttgat tatctaaaac
     3901 aacgtgaaca tcaaacatta tccttgtcct ttttaaatga atattcgcga gtaatatgag
     3961 caatactaat tttgtaaaaa tcaaatattg actctcgccc ttcttgttga gctttcgcat
     4021 gtaaaacatg atttttccat tgcagaactg cgtattcgtt ttcccaccaa gatagcgata
     4081 acatttttcc ttctgtagct agactttgaa aacgttcaat tgaaataaaa ccagctacat
     4141 gacttaatag tgg
//
LOCUS       DAMZFT010000258         4086 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.258, whole genome shotgun sequence.
ACCESSION   DAMZFT010000258 DAMZFT010000000
VERSION     DAMZFT010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4086)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4086)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4086
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.258"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(172..357)
                     /locus_tag="PN977_005110"
                     /pseudo
     CDS             complement(172..357)
                     /locus_tag="PN977_005110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461282.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase family protein"
     gene            408..1099
                     /locus_tag="PN977_005111"
                     /pseudo
     CDS             408..1099
                     /locus_tag="PN977_005111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            1502..1693
                     /locus_tag="PN977_005112"
     CDS             1502..1693
                     /locus_tag="PN977_005112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001349533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmoprotectant transport activator ProQ"
                     /protein_id="HDI6045024.1"
                     /translation="MVTLTINRKPQGIFSKSPATPLQDKTDTAYKMKAGDQIPQLNDK
                     QKPADKTPWRHDKTPEQKP"
     gene            1762..2010
                     /locus_tag="PN977_005113"
     CDS             1762..2010
                     /locus_tag="PN977_005113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001349534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmoprotectant transport activator ProQ"
                     /protein_id="HDI6045025.1"
                     /translation="MGIPDDLIQDIAIRELAFGAGTLHAAVASYVQSPRYYRALIAGG
                     ARYNLNGQPCGEVTPQEQKEAETRLMMLNDRRKARKPR"
     gene            complement(2029..2577)
                     /locus_tag="PN977_005114"
     CDS             complement(2029..2577)
                     /locus_tag="PN977_005114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001167424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6045026.1"
                     /translation="MQITEALLSAPGEIRRFVQQAVDHWPNLLAFHFTLHSAEGNING
                     QQIHAFCTSFYRQVHERITERNHTASPAPPVVLRWLREQHGGATMRCLLLLSQTSICH
                     PRVSATVDEECSQRVDLLQQTWRVISAGGQCRVEKRFQVVRGDTSGQYVALKTVALSL
                     GLPVVIAITHRPVQRCTLITAQ"
     gene            complement(2855..3088)
                     /locus_tag="PN977_005115"
     CDS             complement(2855..3088)
                     /locus_tag="PN977_005115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020245245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family transcriptional regulator"
                     /protein_id="HDI6045027.1"
                     /translation="MLTTTSHDSVLLRADDPLIDMNYITSFTGMTDKWFYKLISEGHF
                     PKPIKLGRSSRWYKSEVEQWMQQRIEESRGAAA"
     gene            complement(3112..3729)
                     /locus_tag="PN977_005116"
     CDS             complement(3112..3729)
                     /locus_tag="PN977_005116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001593693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6045028.1"
                     /translation="MNAIPYFDYSMAPFWPSYQNKIVGVLNRALREQSCSRVRRILLR
                     LPWEHDNAFSCRKTWLGMDFIETVSALMNATPGRDLCWILTRHPEKPEYHVVLCVRQE
                     YFDGPELDRLILDAWSNVLGFASPGEAAPYQKQITRDVVLDSRSPDCEDILKELIWAF
                     SDFARDRRGVRDPEARYLAGNPWYPVAGQLRKHSSADSPAFAQPS"
     gene            3744..3893
                     /locus_tag="PN977_005117"
     CDS             3744..3893
                     /locus_tag="PN977_005117"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6045029.1"
                     /translation="METHDASPPAKDERMVKLKASCTEVVDVITVEPGMADVVCQDSQ
                     ETRQV"
BASE COUNT         1059 a         1051 c          998 g          978 t
ORIGIN      
        1 cacctacgtt accaatactg gcttaatgga tacatacggc ggtcggttct tacggtaaaa
       61 cgacacaacc aaacgcttct tatcctgcaa tgaactgctt tattaccgag tttagtgacc
      121 gcacagacac tcacttttaa gaaaaacaga atggtaaaca aactccatga attacaatag
      181 ctctctggct gccgccacaa tagcttgcgc ggttaacccg tactctctct gtaaaaagtc
      241 ctgagtttca acctggccat accgctcttt aacccccacc cgacgcatgg gcacaggaca
      301 agtttctaca agcacttcag caactgctga acctaatcca ttatgaatac ggtaatgact
      361 ccaattagtt gaattaggag tataatagcc acttttaagg ttctttcatg gtcacagtta
      421 accttcattg ctcctgttgc cagtccgttc aggtttaccg tcacgggaag aacccaaagg
      481 acatgatcga tttcgctgcc gtgactgtca tcgagtgctt cagctcactt accgttacga
      541 ggcccgtaaa ccgggagtta aagagcaaat cactgaaatg gctttcaacg gtgccggagt
      601 tcgcgatact gcccgtacgc tgaaaatcgg cattaatact gtcatccgga ctttaaaaaa
      661 ctctcgccac gaagaataac gtcatacccg gtcgctcatg ctgatatggc actcatctgc
      721 gaacttgatg agcaatggag ttttgtcggc agtaaagcgc gacagcactg gctctggtac
      781 acgtacaaca cgaaaactgg tggcgttctg ggctacactt ttggcctacg gactgattaa
      841 acctgtcgtg aattgctggc gctgcttgcc cttttaccat cggtatgttg accactgacg
      901 aatggggtag ttacaccaga gagttaccga aggagaagca tctgactggt aagattttta
      961 ctcagcgcat tgagcgcaac atctacctct gagaacccga atcaaacgtc tggcccgcag
     1021 aactatctgt ttctcgcgct ctgtagaact tcatgaaaaa gtcatcggtg cctttatcga
     1081 aaaatatatg ttctactaat tggagtcacc accttcgttt gtttaaagaa cctgctggtc
     1141 ctgccattaa tattacctgc tgattgcttc acatccggat tcctcgcact gcgcgcgggt
     1201 tattgtttcg ttttcggcca cggggtcgcc agggctctgt ttgcaggcca tgatggcctg
     1261 acctgtactg tttctcaggc actgagatgt ccgtttacgc catttgttcc cggatatcct
     1321 taacctctga cgtttgttcc ctgcccctct gtctgataac gaatacgctc acaacgcaca
     1381 gtactgaaca ataccgacaa caacatgtct tacatgaaat tcgctgtaac atccaccgga
     1441 aaacattttc agatatcgga tgaggtacta cttccattga cagcgcgagg taacaaaatt
     1501 gatggtaact ctgaccatta accgtaaacc gcagggtatt ttcagtaaat caccggcaac
     1561 gccgctgcag gataaaacgg acacagcata caaaatgaag gccggtgacc agataccaca
     1621 gttgaacgat aaacagaaac ctgctgataa aacaccgtgg cgacatgaca aaacgccaga
     1681 gcaaaaaccg taagcgcatt attcgtcttg tggaactctg gcctgagtca ttcaaccggg
     1741 aaaaaaacaa aaccacttaa agtggggata cctgacgacc tgatacagga tatcgccatc
     1801 cgagaactgg cgtttggcgc tggtacatta catgcagcag tggcgtctta tgtgcagtct
     1861 ccgcgctatt accgtgcgtt aatagcaggt ggcgcccgtt acaacctgaa cggtcagccg
     1921 tgcggggaag tgacaccaca ggaacagaaa gaggcagaga cccgtttaat gatgctgaat
     1981 gaccgacgaa aagccagaaa acctcgttga ctgcacaact gcgctgattc actgagctgt
     2041 aatcaatgta cagcgctgta ccggacgatg ggtaatggcg atcacaaccg gtaaccccag
     2101 agacaatgcg actgttttta acgcaacata ctggccagat gtatcacccc ggacgacctg
     2161 aaaacgcttt tccacccggc attgtccgcc agcacttatc acccgccatg tctgttgcaa
     2221 taaatccacc cgttgcgaac attcttcatc aaccgtggca ctgacccgtg gatggcaaat
     2281 gctcgtctgg ctgagcagca acaggcatcg cattgttgct cctccatgtt gttcccgcaa
     2341 ccagcgtaat accaccggtg gtgctggact ggccgtgtga ttacgttcag taatacgctc
     2401 gtgcacctgt cggtaaaagg aagtacagaa tgcatgaatc tgttgtccgt tgatgtttcc
     2461 ttctgccgaa tggagcgtga agtgaaatgc caacagattt ggccagtggt ccaccgcctg
     2521 ctgaacaaaa cgccggattt cccccggcgc tgaaagtaag gcttcggtta tttgcactat
     2581 tttatccccg ttgaattttg ttaagccggt gcagaccaga cacacggaaa gcgcacatcg
     2641 acaccggcac ggtacgatac aaacaccagt gactggcggg acgaagggaa tgacgaatca
     2701 gtcaggaaat gatgacaaac agttggctgg ccagagggtg ccagcagaaa gatgagcggg
     2761 agttgtcagc gccagcacca gggacagggc tgatttccac aataccagcg ccggcatttc
     2821 gcacattgcc agcgtactgg cattacaaca cgtttcatgc tgctgctcct cgtgattcct
     2881 caattcgctg ctgcatccac tgctccactt cacttttgta ccagcggctg ctgcgcccca
     2941 gtttgatggg tttagggaaa tgaccttcac tgatcagctt gtaaaaccat ttatcggtca
     3001 taccggtgaa actggtgatg tagttcatgt cgatcagggg atcgtcggca cgcagcaata
     3061 cgctgtcgtg gcttgttgtg gtcaacatgg tcagaatgtc ctttgtgaga tttatgaagg
     3121 ttgtgcaaat gcgggggaat ctgctgaact gtgctttctg agctggcctg ctacgggata
     3181 ccagggatta ccggcgaggt aacgggcttc cggatcgcgc acaccacgac gatcgcgggc
     3241 gaaatcactg aacgcccata tcaactcttt gaggatgtct tcgcagtccg gtgaacgact
     3301 gtccagcacc acatcccgtg ttatctgttt ctggtacggt gccgcttcac ccggtgacgc
     3361 gaagcccagt acgttgctcc aggcatccag aatcagccgg tccagttcag ggccatcgaa
     3421 atactcctgt ctgacgcaca gcaccacgtg gtattccggc ttttcaggat gacgggttag
     3481 gatccagcag agatcacgtc cgggggttgc attcatcagc gcactgacgg tttcgataaa
     3541 atccatgcct aaccaggtct ttctgcaact gaaggcgttg tcatgttccc acggcagacg
     3601 aagcaggatc cgccgtaccc gggagcagga ctgttcacgc agcgcacgat tgagaacgcc
     3661 gacgattttg ttctgataag atggccagaa gggtgccatg ctatagtcga aataagggat
     3721 agcattcata agatatgctc catatggaaa cacacgacgc atccccgcca gcgaaagacg
     3781 agcgtatggt gaaactgaag gcgtcgtgta ctgaggttgt tgatgttata accgtggaac
     3841 cggggatggc agatgtcgta tgccaggaca gtcaggagac cagacaggtg taaaattcct
     3901 gaggtggtga tctccgttga cttcggttca catcattaat catcccggta agtttttacc
     3961 gacggattgt ttccaggagc ggaagcagat aaaaataccg tttcatgact tcagggcgat
     4021 tagcagaaaa cgatctaagg gcgttggctg acgcccttac cgtgacatac actctggtac
     4081 cggatg
//
LOCUS       DAMZFT010000259         3982 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.259, whole genome shotgun sequence.
ACCESSION   DAMZFT010000259 DAMZFT010000000
VERSION     DAMZFT010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3982)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3982)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3982
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.259"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..1328
                     /gene="ybiO"
                     /locus_tag="PN977_005118"
     CDS             <1..1328
                     /gene="ybiO"
                     /locus_tag="PN977_005118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="mechanosensitive channel protein"
                     /protein_id="HDI6045030.1"
                     /translation="IIMLCMTVWALYLIFRNKKEITQHLLNFAEHSLAFFSLFIRAFA
                     LVWHWLASAYFIVLFFFSLFDPGNSLKFMMGATVRSLAIIGIAAFVSGMFSRWLAKTI
                     TLSPHTQRNYPELQKRLNGWLSAALKTARILTVCVAVMLLLSAWGLFDFWNWLQNGAG
                     QKTVDILIRIALILFFSAVGWTVLASLIENRLASDIHGRPLPSARTRTLLTLFRNALA
                     VIISTITIMIVLSEIGVNIAPLLAGAGALGLAISFGSQTLVKDIITGVFIQFENGMNT
                     GDLVTIGPLTGTVERMSIRSVGVRQDTGAYHIIPWSSITTFANFVRGIGSVVANYDVD
                     RHEDADKANQALKDAVAELMENEEIRGLIIGEPNFAGIVGLSNTAFTLRVSFTTLPLK
                     QWTVRFALDSQVKKHFDLAGVRAPVQTYQVLPAPGATPAEPLPPGEPTL"
     gene            complement(1325..2251)
                     /gene="rlmF"
                     /locus_tag="PN977_005119"
     CDS             complement(1325..2251)
                     /gene="rlmF"
                     /locus_tag="PN977_005119"
                     /EC_number="2.1.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415328.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(1618)-N(6))-methyltransferase
                     RlmF"
                     /protein_id="HDI6045031.1"
                     /translation="MSAQKPGLHPRNRHHSRYDLATLCQVNPELRQFLTLTPAGEQSV
                     DFANPLAVKALNKALLAHFYAVANWDIPDGFLCPPVPGRADYIHHLADLLAEATGSIL
                     ANASILDIGVGANCIYPLIGVHEYGWRFTGSETSSQALSSAQAIISANPGLNRAIRLR
                     RQKESGAIFNGIIHKNEQYDATLCNPPFHDSAAAARAGSERKRRNLGLNKDDALNFGG
                     QQQELWCEGGEVAFIKKMIEESKGFAKQVMWFTSLVSRGENLPPLYRALTEVGAVKVV
                     KKEMAQGQKQSRFIAWTFMNDEQRRRFVNRQR"
     gene            2527..2787
                     /locus_tag="PN977_005120"
     CDS             2527..2787
                     /locus_tag="PN977_005120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HDI6045032.1"
                     /translation="MKKCLTLLIATVLSGISLTAYAAQPMSNLDSGQLRPAGTVSATG
                     ASNLSDLEDKLAEKAREQGAKGYVINSAGGNDQMFGTAIIYK"
     gene            3052..>3982
                     /locus_tag="PN977_005121"
     CDS             3052..>3982
                     /locus_tag="PN977_005121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HDI6045033.1"
                     /translation="MENNRNFPARQFHSLTFFAGLCIGITPVAQALAAEGQINADDTL
                     VVEASTPSLYAPQQSADPKFSRPVADTTRTMTVISEQVIKDQGATNLTDALKNVPGVG
                     AFFAGENGNSTTGDAIYMRGADTSNSIYIDGIRDIGSVSRDTFNTEQVEVIKGPSGTD
                     YGRSAPTGSINMISKQPRNDSGIDASASIGSAWFRRGTLDVNQVIGDTTAVRLNVMGE
                     KTHDAGRDKVKNERYGVAPSVAFGLGTANRLYLNYLHVTQHNTPDGGIPTIGLPGYSA
                     PSAGTAALNHSGKVDTHNFYGTDSDYDDSTTDTA"
BASE COUNT          931 a         1049 c         1007 g          995 t
ORIGIN      
        1 tcatcattat gctgtgcatg acggtctggg cgctgtacct gatctttcgt aataaaaaag
       61 agattaccca gcatttgctc aacttcgcgg agcattcgct ggcctttttc agcctgttta
      121 tccgcgcctt tgcgctggtg tggcactggc tggcaagcgc ctattttatc gtgctgtttt
      181 tcttttcgtt gttcgatccg ggcaacagcc tgaaatttat gatgggtgca acggtgcgca
      241 gcctggcgat tattggtatc gcagcatttg tttccggtat gttttcccgc tggctggcga
      301 aaaccatcac cctctcgcca catactcagc gtaactatcc ggagctgcaa aaacggttga
      361 atggctggct gtcggcggcg ctgaaaacgg cgcgtattct gacagtctgc gtggcggtaa
      421 tgctgctgtt gagcgcatgg ggattgttcg atttctggaa ctggctgcaa aacggcgcgg
      481 ggcagaaaac cgtagatatc ctgatccgta tcgcactcat tcttttcttc tcggcggttg
      541 gctggacagt gctcgccagt ttgatcgaaa accggctggc ttcggatatt catggccgcc
      601 cgctacccag cgcccgtacg cgtaccctgc tgacgctgtt tcgtaacgcg ctggcggtga
      661 ttatcagtac catcaccatc atgattgtgt tgtcggaaat cggcgttaat atcgcgccat
      721 tgctggcagg tgccggggca ttaggtctgg ctatctcgtt tggttcgcaa acgctggtga
      781 aagatattat caccggggta tttattcagt ttgaaaacgg catgaacact ggagatttgg
      841 tgactatcgg gccgttgacc ggcactgtgg aacggatgtc gattcgctcc gtgggcgtgc
      901 gtcaggatac cggggcgtat cacatcattc cgtggtcttc gataactacc tttgctaact
      961 tcgtccgcgg cattggttcg gtggtggcaa attacgatgt tgatcgccat gaagatgctg
     1021 ataaagccaa tcaggcactg aaagatgcgg tagcggaatt aatggaaaac gaagaaattc
     1081 gcgggctgat tattggtgaa ccgaattttg ccgggattgt cggcttaagc aataccgcgt
     1141 ttacactgcg tgtttcgttc accacgctgc cactcaaaca gtggacggtc cgctttgccc
     1201 tcgacagcca ggtgaaaaaa catttcgacc tggcgggcgt tcgcgcgcca gtgcagactt
     1261 atcaggtgct gcctgctccg ggcgcgaccc cggctgaacc gttgccgccg ggggaaccaa
     1321 cgctttaacg ctggcgattg acaaaacggc ggcgctgctc gtcgttcata aaggtccagg
     1381 ctataaagcg gctctgcttt tgcccctggg ccatctcttt tttgaccact ttcaccgcgc
     1441 ccacttccgt cagagcacga tacaacggcg gtaagttttc gccacgagat accagcgagg
     1501 taaaccacat cacctgcttc gcgaagcctt tgctctcttc aatcatcttt ttaataaaag
     1561 cgacttcacc gccttcacac cacaactcct gttgctgacc accaaaattc agtgcatcgt
     1621 ctttgtttag ccccaggtta cggcgtttac gttcactacc tgcccgtgcc gcagcggcgg
     1681 aatcgtggaa tggcgggtta cacaaggtcg cgtcgtattg ctcgttttta tggatgatgc
     1741 cgttaaaaat cgccccactc tctttttgcc gacgcagacg aatggcgcgg ttaagccccg
     1801 gattagcact gatgatcgcc tgcgcactgc ttaacgcctg gctgctggtt tcactaccag
     1861 taaaacgcca gccatattca tgtacgccaa tcagcggata aatacagttc gcaccaacgc
     1921 cgatatccag aatgctggca ttagctagaa ttgatcctgt tgcttctgcc agtaaatcgg
     1981 caaggtgatg aatataatcc gcccggcccg gtactggtgg gcagagaaaa ccgtcgggga
     2041 tatcccagtt cgctacggcg taaaaatggg ccagcaacgc cttattgagc gccttcaccg
     2101 ccagcggatt ggcaaagtct acgctttgct ccccggcggg tgtaagcgtg aggaattgcc
     2161 tgagttcagg attgacctga caaagcgtgg cgagatcgta gcggctgtga tgacggttgc
     2221 gcggatgcaa ccccggtttc tgggcggaca tagcattctc ctttaagcag cggcgtaaga
     2281 tacccgcata acgcgacagg gtaaatatct ggctgcgttt atcatttcta taaaccttca
     2341 catggctatg ttttatggca attcatcata aaaatcagca ttatgcatat cattgatttg
     2401 tcttgttttg taaaaatata tcgttcaaaa aacaagcaca tctctgtcaa ttttttgcgt
     2461 cattttcatc gcccaggcga cctacactta tcaggcacta cccgttgtat ttatgaggat
     2521 gtcattatga aaaagtgcct cacactactg attgccaccg tcctgagcgg aatctctctc
     2581 acggcttatg ccgcgcaacc gatgagtaac ctggacagcg gtcagctacg gcccgccggt
     2641 accgtttcgg caactggcgc atcaaaccta agcgatctgg aggataaact ggcagaaaaa
     2701 gcgcgcgaac aaggcgcgaa aggttatgtc attaattccg caggcggaaa tgaccagatg
     2761 ttcggtactg caatcatcta caaataaccc caccaacgta actgcattcg cctctggatg
     2821 cagttttcgt ccgatttgta agcaaaaaac atcaaatatg aacgcaatgt aaataaatgt
     2881 atttcttttt cgcgcaatgg gtgatagaaa atcgcgccaa gtgataatgc ttatcaaaat
     2941 tattatcact tgcacgaacg ctatcacgag attaacaatg gcatcgcatc cgcagagatg
     3001 ctttctcgtg gcagtgaaaa tttcaacata taagaaaaag tcacctgcaa aatggaaaac
     3061 aatcgcaatt tccctgccag acaatttcat tcgctcacgt tctttgccgg tctttgtatt
     3121 ggcatcacgc ctgtggctca ggctctcgcc gccgaagggc aaattaacgc ggatgacacg
     3181 ctggttgtcg aagcatcaac gccttcgctt tatgcgccac aacaatctgc cgatccgaaa
     3241 ttctcgcgtc cggtagcgga tactacccgc acgatgacgg tgatttctga acaagtgatt
     3301 aaagatcagg gtgcaaccaa ccttaccgac gcgctcaaaa acgtccccgg cgtgggtgcg
     3361 ttttttgcgg gtgagaacgg taactccacc actggcgacg ccatttatat gcgtggtgcc
     3421 gatacctcta acagtattta tattgatggc attcgcgata tcggcagcgt ctcgcgcgac
     3481 accttcaata ccgagcaggt cgaagtgatt aaagggccgt ccggcaccga ctacgggcgc
     3541 agcgcaccga cgggctcgat caatatgatc agcaagcagc cgcgcaatga ttcgggcatt
     3601 gacgcctccg cgagtattgg cagcgcctgg ttccgccgcg gcacgctgga cgtcaatcag
     3661 gtcattggtg ataccaccgc ggtgcgcctg aatgtaatgg gcgaaaaaac gcacgatgcc
     3721 ggacgcgaca aagtcaaaaa tgagcgttac ggcgtcgccc cttctgtcgc ttttggcctt
     3781 ggtacagcga atcgtttgta tcttaattat ctgcatgtca cccagcacaa cacgccagac
     3841 ggtggcattc cgactatcgg tttaccgggc tattctgccc catctgcggg aacagcggcc
     3901 ctgaatcatt ccggaaaagt tgatacgcat aacttttacg gcacggattc cgattacgac
     3961 gattcgacca ccgacaccgc ta
//
LOCUS       DAMZFT010000260         3957 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.260, whole genome shotgun sequence.
ACCESSION   DAMZFT010000260 DAMZFT010000000
VERSION     DAMZFT010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3957)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3957)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3957
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.260"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..526
                     /locus_tag="PN977_005122"
     CDS             <1..526
                     /locus_tag="PN977_005122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDI6045034.1"
                     /translation="INENEVLDWLGGQFLGVPVSALIMMVLFALFVFISRKTAFGRSV
                     FAVGGNATAAQLCGINVRRVRILIFTLSGLLAAVTGILLAARLGSGNAGAANGLEFDV
                     IAAVVVGGTALSGGRGSLFGTLLGVLVITLIGNGLVLLGINSFFQQVVRGVIIVVAVL
                     ANILLTQRSSKAKR"
     gene            559..1653
                     /locus_tag="PN977_005123"
     CDS             559..1653
                     /locus_tag="PN977_005123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HDI6045035.1"
                     /translation="MKINRIVNEGFMKTMLAAYLPGNSTVDLREVAVPTPGINQVLIK
                     MKSSGICGSDVHYIYHQHRATAAAPDKPLYQGFINGHEPCGQIVAMGQGCRHFKEGDR
                     VLVYHISGCGFCPNCRRGFPISCTGEGKAAYGWQRDGGHAEYLLAEEKDLILLPDALS
                     YEDGAFISCGVGTAYEGILRGEVSGSDNVLVVGLGPVGMMAMMLAKGRGAKRIIGVDM
                     LPERLAMAKQLGVMDHGYLATTEGLPQIIAELTHGGADVALDCSGNAAGRLLALQSTA
                     DWGRVVYIGETGKVEFEVSADLMHHQRRIIGSWVTSLFHMEKCAHDLTDWKLWPRNAI
                     THRFSLEQAGDAYALMASGKCGKVVINFPD"
     gene            1665..2537
                     /locus_tag="PN977_005124"
     CDS             1665..2537
                     /locus_tag="PN977_005124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase"
                     /protein_id="HDI6045036.1"
                     /translation="MTIYTLSHGPLKLDVSDRGGVIEGFWRDATPLLRPGKKSGVATD
                     ASCFPLVPFANRVSGNRFVWQGREYQLQPNVEWDAHYLHGDGWLGEWQCVSRSDDSLC
                     LVYEHLSGVYHYRVSQAFHLTADTLTVTLAVTNQGAETLPFGTGWHPYFPLSPQTRIQ
                     AQASGYWLEREQWLAGEFCEQLPQELDFNQLTPLPRQWVNNGFAGWNGKARIEQPQEG
                     YAIIMETTRPAPCYFIFVSDPAFDKGYAFDFFCLEPMSHAPDDHHRPEGGDLIALAPG
                     ESTTSEMSLRVALL"
     gene            complement(2585..3007)
                     /locus_tag="PN977_005125"
     CDS             complement(2585..3007)
                     /locus_tag="PN977_005125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311436.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HDI6045037.1"
                     /translation="MNSLRYFDFGAARPVLLLIARIAVVLIFIIFGFPKMMGFDGTVQ
                     YMASLGAPMPMLAAIIAVVMEVPAAILIVLGFFTRPLAVLFIFYTLGTAVIGHHYWDM
                     TGDAVGPNMINFWKNVSIAGAFLLLAITGPGAISLDRR"
     gene            complement(3104..>3957)
                     /locus_tag="PN977_005126"
     CDS             complement(3104..>3957)
                     /locus_tag="PN977_005126"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phenylpropionate dioxygenase ferredoxin
                     reductase subunit"
                     /protein_id="HDI6045038.1"
                     /translation="LDALGERCFTLRHAGDAARLREVLQPERSVVIVGAGTIGLELAA
                     SATQRGCKVTVIELAATVMGRNAPPPVQHYLLQRHQQAGVRILLNNAIEHVVDGENVE
                     LTLQSGETLRADVVIYGIGISANDQLAREANLDTANGIVIDEACRTCDPAIFAGGDVA
                     ITRLDNGALHRCESWENANNQAQIAASAMLGLPLPRLPPPWFWSDQYSDNLQFIGDMH
                     GDDWLCRGNPETQKAIWFNLQNGVLIGAVTLNQGREIRPIRKWIQSGKTFDAKLLTDE
                     DIALKSL"
BASE COUNT          883 a         1000 c         1136 g          938 t
ORIGIN      
        1 aattaacgaa aacgaagtgc tggactggct gggcggacaa tttctcggtg tgccggtatc
       61 cgcgctgatc atgatggtgt tatttgcgct gtttgtgttc attagccgca aaaccgcctt
      121 cgggcgctcg gtttttgctg ttggcggtaa tgccactgca gcgcagttgt gcggtattaa
      181 cgttcgtcgg gtacgcattc ttatttttac cctttcggga ttattagcgg cggtgaccgg
      241 cattttgttg gcggcgcgcc tcggttccgg taacgcaggt gccgcaaacg gtctggagtt
      301 tgacgtcatc gccgcggtcg tcgtcggcgg tacggcgctt tccggtggtc gcggctcgtt
      361 gttcggtacg ctgcttggcg tactggtgat tacgctaatc ggtaacggtc tggtgctgct
      421 cgggattaac tcctttttcc agcaggtggt gcgcggcgtc atcatcgtgg tggcggtgct
      481 ggcgaatatc ttgctgaccc agcgtagcag taaagcgaaa cgctaaacct taacgcccct
      541 ggcgcgggtg cggcagggat gaaaattaat cgtattgtta atgagggatt tatgaaaacg
      601 atgctggcag cttatttacc aggaaattcg accgtcgatc tgcgggaagt tgcggtgccg
      661 acgccgggta ttaaccaggt actgatcaaa atgaaatcct ccggaatttg cggaagcgat
      721 gtccactata tctaccacca gcaccgcgct acggcggcgg cacccgataa accgttatac
      781 cagggattta tcaacggtca tgaaccgtgc gggcaaattg tggcgatggg gcaaggctgc
      841 cgccatttta aagagggcga ccgtgtgctg gtgtatcaca tttctggctg tggtttttgc
      901 ccgaactgcc gtcgtggttt tcctatctct tgtactggcg aaggaaaagc ggcttacggc
      961 tggcagcgtg acggcggcca tgctgaatac ctgctggcgg aagaaaaaga tctgatcctc
     1021 ctgccggatg cactgagtta cgaagatggt gcgtttatca gttgcggcgt tggtacagcg
     1081 tatgaaggga ttttgcgcgg cgaagtttcc ggcagtgata acgtgctggt ggtcggtctg
     1141 gggccggtcg gcatgatggc gatgatgctg gcaaaaggtc gcggtgcaaa aaggatcatc
     1201 ggcgttgata tgttgccgga acgtctggcg atggcgaaac agttaggagt gatggatcac
     1261 ggctatttag cgactaccga aggtctgccg cagataatcg ccgaactcac tcacggcggc
     1321 gcggatgttg cgcttgattg ttccggtaat gccgcaggtc gcttactggc actgcaatcc
     1381 accgctgact ggggacgggt ggtttacatc ggggaaaccg gaaaagtgga gttcgaggtt
     1441 agcgctgatc tgatgcatca tcaacggcgg attattggct cctgggtgac cagtctgttc
     1501 catatggaaa aatgcgccca cgatttaacg gactggaaac tgtggccgcg taatgccatt
     1561 acccatcgct tctcactgga acaggcagga gatgcctatg cgctgatggc gagcggcaaa
     1621 tgcgggaaag ttgtgattaa cttcccggat taaggagtta gcggatgacc atctatacct
     1681 tatctcacgg gccgctaaag ctggacgttt ctgaccgggg cggcgtcatc gaaggcttct
     1741 ggcgtgacgc gacgccgctc ttacgtcccg gtaaaaaaag cggtgtggca accgatgcct
     1801 cttgttttcc gctggtgccg tttgcaaacc gggtgagcgg caatcgtttt gtctggcagg
     1861 ggcgtgagta tcaactgcaa ccgaatgttg agtgggatgc gcattatcta cacggcgatg
     1921 gctggttggg cgagtggcaa tgtgtctcgc gcagcgatga tagcttgtgt ctggtgtatg
     1981 aacatctcag cggtgtttat cactatcggg taagccaggc atttcattta acggcggata
     2041 cgctgacagt gacgctcgct gtcaccaatc aaggggcaga gacgctgcca tttggtaccg
     2101 gctggcatcc ttattttccg ttgtcgccgc aaacgcggat tcaggcacag gcgagcggtt
     2161 actggctgga gcgggagcag tggctggcgg gtgagttttg cgagcagcta ccgcaggaac
     2221 tggattttaa ccagctcacg ccgttgccgc gccagtgggt aaacaatggt tttgccggat
     2281 ggaatggtaa ggcccgcatc gagcagccgc aggagggtta tgccatcatc atggaaacga
     2341 cgcggcctgc accgtgttat ttcatctttg tttccgaccc ggcgtttgat aaaggatatg
     2401 cgtttgattt cttctgtctg gaaccgatga gccatgcgcc agatgatcat catcgcccgg
     2461 aaggtggtga tctcattgcg cttgcaccag gggaatcaac aacttcagag atgtcgttgc
     2521 gggttgcgtt actgtaattt ataaaatgcc ggataaggcg ttcacgccgc atccggcatc
     2581 tttcctaacg ccgatcgaga gaaatcgccc ccggcccggt aattgccaac agcaggaatg
     2641 cgccagcgat actgacattc ttccagaaat taatcatatt tggcccaacc gcatcgccgg
     2701 tcatatccca gtaatggtga ccaattaccg ctgtacccag cgtgtagaaa ataaacagca
     2761 ccgccagcgg acgggtgaaa aagccaagca cgattaatat cgcggcgggc acttccataa
     2821 ctaccgcaat aatcgctgcc agcatcggca ttggcgcgcc cagcgaggcc atatattgga
     2881 ccgtaccgtc aaagcccatc attttgggaa aaccaaaaat aatgaaaatt aagaccacgg
     2941 cgatacgggc aattaataac aaaacggggc gtgcagcacc aaaatcaaaa taacgtagtg
     3001 agttcataac caatcccttt gcaagataat gtgatttaaa cgtaatacac actctggcac
     3061 ctcgccacgc cccaggagtt tagatattcg ctaattatcc tggttacagt gatttaagcg
     3121 cgatgtcctc atctgtcagc agtttcgcat caaacgtttt gccgctctgg atccatttgc
     3181 ggattgggcg aatctcacgg ccctgattca gcgtgactgc accgataagc acgccgtttt
     3241 gcagattaaa ccaaatcgcc ttctgagttt ccgggttgcc acgacaaagc cagtcatcgc
     3301 catgcatatc gccaataaac tgtaagttat cactgtactg atcgctccag aaccacggcg
     3361 gcggcagtcg cggaagcggt agccccaaca ttgcggaagc ggcaatttgc gcctggttat
     3421 tggcgttttc ccagctttcg cagcggtgta gtgcaccatt atcaagacgg gtgattgcca
     3481 catcgccacc ggcaaagatc gcgggatcgc aggtgcggca agcctcatca atgacaatgc
     3541 cattggcagt atcaaggttg gcctcgcgag ccagttggtc gttggcgctg ataccaatac
     3601 cgtaaatcac cacatcggcc cgaagcgttt cgccactttg cagcgtcagt tctacgtttt
     3661 caccatcgac cacatgttca atggcattat tgagcagaat gcgcacacca gcctgctgat
     3721 ggcgctgtaa aagatagtgt tgcacgggcg gtggtgcatt acggcccatg acggttgccg
     3781 ccagttcaat cactgtcacc ttacatccac gctgcgtggc gctggcagcc agttccagac
     3841 caatagttcc ggcaccgaca atcacgactg accgttcggg ctgcagaact tctcgcagtc
     3901 tggcggcatc gccggcatgg cgcagagtaa agcagcgttc ccccagtgca tcaagca
//
LOCUS       DAMZFT010000261         3872 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.261, whole genome shotgun sequence.
ACCESSION   DAMZFT010000261 DAMZFT010000000
VERSION     DAMZFT010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3872)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3872)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3872
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.261"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(33..98)
                     /gene="yobI"
                     /locus_tag="PN977_005127"
     CDS             complement(33..98)
                     /gene="yobI"
                     /locus_tag="PN977_005127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response small protein YobI"
                     /protein_id="HDI6045039.1"
                     /translation="MYIFITHFFTEYVIFKYLLPI"
     gene            169..771
                     /locus_tag="PN977_005128"
     CDS             169..771
                     /locus_tag="PN977_005128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebB family permuted papain-like enzyme"
                     /protein_id="HDI6045040.1"
                     /translation="MNINYPAEYEIGDIVFTCIGTALFGQISAASNCWSNHVGIIIGH
                     NGEDFLVAESRVPLSTITTLSRFIKRSSNQRYAIKRLDAGLTEQQKQRIVEQVPSRLR
                     KLYHTGFKYESSRQFCSKFVFDIYKEALCIPVGEIETFGELLNSNPNAKLTFWKFWFL
                     GSIPWERKTVTPASLWHHPGLVLIHAEGVETPQPELTEAV"
     gene            complement(773..1294)
                     /gene="ruvC"
                     /locus_tag="PN977_005129"
     CDS             complement(773..1294)
                     /gene="ruvC"
                     /locus_tag="PN977_005129"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005068993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HDI6045041.1"
                     /translation="MAIILGIDPGSRVTGYGVIRQVGRQLSYLGSGCIRTKVDDLPSR
                     LKLIYAGVTEIITQFQPDYFAIEQVFMAKNADSALKLGQARGVAIVAAVNQELPVFEY
                     AARQVKQTVVGIGSAEKSQVQHMVRTLLKLPANPQADAADALAIAITHCHVSQNAMQM
                     SESRLNLARGRLR"
     gene            complement(1329..2069)
                     /locus_tag="PN977_005130"
     CDS             complement(1329..2069)
                     /locus_tag="PN977_005130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDI6045042.1"
                     /translation="MAGHSKWANTRHRKAAQDAKRGKIFTKIIRELVTAAKLGGGDPD
                     ANPRLRAAIDKALSNNMTRDTLNRAIARGVGGDDDANMETIIYEGYGPGGTAIMIECL
                     SDNRNRTVAEVRHAFSKCGGNLGTDGSVAYLFSKKGVISFEKGDEDTIMEAALEAGAE
                     DVVTYDDGAIDVYTAWEEMGKVRDALEAAGLKADSAEVSMIPSTKADMDAETAPKLMR
                     LIDMLEDCDDVQEVYHNGEISDEVAATL"
     gene            complement(2098..2550)
                     /gene="nudB"
                     /locus_tag="PN977_005131"
     CDS             complement(2098..2550)
                     /gene="nudB"
                     /locus_tag="PN977_005131"
                     /EC_number="3.6.1.67"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate diphosphatase"
                     /protein_id="HDI6045043.1"
                     /translation="MTDMAYKRPVSILVVIYAQDTKRVLMLQRRDDPDFWQSVTGSVE
                     EGETAPQAAMREVKEEVTIDVVAEQLTLIDCQRTVEFEIFSHLRHRYAPGVTRNTESW
                     FCLALPHERQVVFTEHLAYKWLDAHAAAALTKSWSNRQAIEQFVINAA"
     gene            complement(2543..>3872)
                     /gene="aspS"
                     /locus_tag="PN977_005132"
     CDS             complement(2543..>3872)
                     /gene="aspS"
                     /locus_tag="PN977_005132"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HDI6045044.1"
                     /translation="RRFMDDHGFLDIETPMLTKATPEGARDYLVPSRVHKGKFYALPQ
                     SPQLFKQLLMMSGFDRYYQIVKCFRDEDLRADRQPEFTQIDVETSFMTAPQVREVMEA
                     LVRHLWLEVKGVDLGDFPVMTFAEAERRYGSDKPDLRNPMELTDVADLLKSVEFAVFA
                     GPANDPKGRVAALRVPGGASLTRKQIDEYGNFVKIYGAKGLAYIKVNERAKGLEGINS
                     PVAKFLNAEIIEAILDRTAAQDGDMIFFGADNKKIVADAMGALRLKVGKDLGLTDESK
                     WAPLWVIDFPMFEDDGEGGLTAMHHPFTSPKDMTAAELKAAPENAVANAYDMVINGYE
                     VGGGSVRIHNGDMQQTVFGILGINEEEQREKFGFLLDALKYGTPPHAGLAFGLDRLTM
                     LLTGTDNIRDVIAFPKTTAAACLMTEAPSFANPTALAELSIQVVKKAENN"
BASE COUNT          919 a         1048 c          916 g          989 t
ORIGIN      
        1 gaaaaatgac gcagtataat tttttctaca ggttatattg gaagcaaata tttaaatatt
       61 acatattcag tgaagaaatg cgtaataaaa atatacattg cgcctcctga aaaaataaat
      121 tttttatgct attacgtata ttcatatcta tttcaatgga atgacaacat gaatattaat
      181 taccctgctg aatatgaaat tggtgatatc gtctttacat gtataggtac tgccttattt
      241 ggccaaatat cagctgcatc aaattgctgg agtaatcacg tcggtatcat tatcggtcat
      301 aacggtgaag acttcctggt tgcagaaagc cgggttcccc tttcaactat caccacgcta
      361 tctcgtttta ttaaacgttc ttctaatcaa cgctatgcta taaagcgatt agacgccggg
      421 ctgacagaac aacaaaaaca acgaattgtt gaacaggttc cctcccggct acgcaaactt
      481 taccacaccg gttttaaata cgaatcttcg cgccagttct gttcaaaatt tgtttttgat
      541 atttataaag aggcgctatg tattccggtg ggtgaaatag agacgtttgg agaactgtta
      601 aacagcaatc cgaatgcaaa actcactttc tggaaattct ggttcctggg ttctattccg
      661 tgggagcgta aaaccgtcac gccagccagt ttgtggcatc atccgggttt agtgttgatt
      721 catgcggagg gagtggaaac gcctcagcct gaactgaccg aggcggtata acttaacgca
      781 gtcgccctct cgccaggttc agccgcgatt cgctcatttg catcgcattc tgactaacgt
      841 ggcagtgggt gatggcaatc gccagcgcat cggcggcatc cgcctgtgga ttagcaggca
      901 gtttcagcaa ggtgcggacc atatgctgca cctggctttt ttcggcacta ccaataccta
      961 ccaccgtttg ctttacctga cgtgccgcat attcaaatac cggcaattcc tgattcaccg
     1021 ccgccacaat cgccacgccg cgcgcctgcc ccagtttcag ggctgagtca gcgttcttcg
     1081 ccataaagac ctgttcaatg gcgaaataat caggctggaa ctgggtgatg atttccgtca
     1141 cgcccgcata gatgagcttc agacgagacg gtaaatcatc cactttggtg cgtatgcatc
     1201 cgctacccag gtaggacagt tgcctgccta cctggcggat gacgccatag ccggtcacgc
     1261 gcgaacccgg gtcaatgccg agaataatag ccatcacgcg tctccgtttt gctgtttagc
     1321 aggcctcatc agagagtcgc tgcaacctca tcagagattt caccgttatg gtaaacttcc
     1381 tgcacgtcgt cgcaatcttc cagcatatcg atcagacgca tcagtttcgg tgcggtttct
     1441 gcatccatat cagctttggt ggacgggatc atggaaactt ccgcgctgtc tgctttcaga
     1501 cctgccgctt ccagagcgtc gcgtactttg cccatttctt cccatgcagt gtagacatca
     1561 atcgcgccgt catcataggt cacaacgtct tcagcaccgg cttccagggc tgcttccatg
     1621 atggtgtctt catcgccttt ctcgaaggag atcacgcctt ttttgctgaa cagataagct
     1681 acggaaccat cagtaccgag gttaccgcca catttgctga atgcatgacg cacttcagca
     1741 acggtacggt tgcggttgtc agacagacat tcaatcatga ttgccgtgcc gccaggaccg
     1801 taaccttcgt agatgatggt ttccatgttt gcatcatcat caccacccac acctcgtgca
     1861 attgcgcggt tcagagtgtc acgggtcatg ttgttagaca gtgctttatc aattgctgca
     1921 cgcaaacgcg ggttagcgtc cggatcacca ccgcccagct tagccgcggt taccagctca
     1981 cgaatgattt tagtgaagat tttaccgcgc ttagcatcct gcgcagcttt acgatgtctg
     2041 gtgttggccc atttactatg acctgccata aaaatatctc cagatagccc tgcctgttca
     2101 ggcagcgtta attacaaact gttcaatcgc ctgccggttg ctccaggact tagtgagcgc
     2161 cgccgcagca tgcgcatcaa gccacttgta ggccagatgt tcagtgaaga cgacctggcg
     2221 ttcgtgcgga agcgcaagac agaaccatga ttccgtatta cgcgtcacgc ccggcgcata
     2281 gcgatgacgt aaatgtgaaa aaatttcaaa ttctaccgtg cgctgacagt caattaaggt
     2341 cagttgttca gcgacaacat cgatggtgac ctcttccttt acttcgcgca tggcagcttg
     2401 cggcgcggtt tcaccctctt ccacgctgcc ggttaccgac tgccagaaat cgggatcgtc
     2461 acgccgctgc aacatcagca cccgtttcgt atcttgtgca tagatgacca ctaagatcga
     2521 aacgggacgc ttataagcca tatcagttat tctcagcctt cttcacaacc tgaatgctca
     2581 gctcagccag tgcagtcggg ttagcaaagc tcggtgcttc agtcatcaga cacgctgccg
     2641 ccgtggtttt cgggaaggcg ataacgtcac ggatattgtc ggtgccggtc agcagcatgg
     2701 tcaaacggtc aagaccaaat gccagacctg cgtgcggcgg agtaccgtat ttcagggcgt
     2761 cgagcaggaa gccgaatttc tcgcgctgtt cctcttcgtt gatacccaga ataccaaaca
     2821 ccgtctgctg catatcacca ttatggatac gcacagaacc accgcccact tcgtaaccat
     2881 tgatgaccat atcgtaagcg ttcgccaccg cattttccgg tgcagctttc agctctgccg
     2941 cagtcatgtc tttcggtgag gtgaacggat ggtgcattgc cgtcaggccg ccttcaccgt
     3001 cgtcttcaaa catcgggaag tcgataaccc acagcggtgc ccatttgctt tcgtcggtca
     3061 gaccaaggtc tttacccact ttcaggcgca gtgcacccat cgcgtcggca acaattttct
     3121 tgttgtcggc accgaagaaa atcatatcgc catcttgcgc ggcagtacga tccaggatgg
     3181 cttcgatgat ttctgcatta aggaacttcg ctaccgggct gttgatacct tccagacctt
     3241 tcgcgcgttc gttaactttg atgtacgcca gacctttcgc gccgtagatt ttaacgaagt
     3301 taccgtattc gtcgatctgt ttacgggtca gcgatgcgcc gcccggaacg cgcagagccg
     3361 ctacgcgacc tttcggatcg ttcgccggac ctgcaaatac tgcaaattca acggatttca
     3421 gcagatcggc aacgtcggtc agttccatcg ggttacgcag atccggttta tcagaaccat
     3481 aacgccgttc tgcttctgca aaggtcatta ccgggaaatc gcccagatcc acgcccttca
     3541 cttccagcca cagatgacgc accagcgctt ccatcacttc acgcacttgc ggcgcggtca
     3601 tgaaagaggt ttccacatcg atctgggtaa attcaggctg acgatcagcg cgcaggtctt
     3661 cgtcacggaa gcatttaacg atctgatagt aacggtcaaa accggacatc atcagcaact
     3721 gtttgaacag ctgcggggat tgcggcagcg cgtagaattt acctttgtgc acacgagaag
     3781 gcaccaggta gtcacgcgcg ccttccggcg tggctttggt cagcatcgga gtttcgatgt
     3841 caaggaaccc gtggtcgtcc ataaaacgac gc
//
LOCUS       DAMZFT010000262         3837 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.262, whole genome shotgun sequence.
ACCESSION   DAMZFT010000262 DAMZFT010000000
VERSION     DAMZFT010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3837)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3837)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3837
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.262"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(<1..1167)
                     /locus_tag="PN977_005133"
     CDS             complement(<1..1167)
                     /locus_tag="PN977_005133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001569357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent DNA methyltransferase"
                     /protein_id="HDI6045045.1"
                     /translation="MNKLSMGVFRCSSVSEILKYIRAITSHRAPIKYGVEKVEGKSYD
                     RLRREANQKAIDLLNSLVDGATLTDEQRQILAGYTGEGGIGGSVSEYYTPKPIAEGVW
                     EIMKLYGADVGNTLEPSAGTGVFNETKPVGTVMTATEISSVSGRINQLLHPEDSVQIS
                     PFEQLAVSTPNDSFDHVVGNVPFGGRDNTRNIDKPYAEETDMGSYFMLRMLDKIKPGG
                     FMCVIVPPSIVSGSNMKRLRLRLSRKAEFLGAHRLPTGTFDANGTSTVVDVVLMRKHP
                     AEMAEKIPLVDEGTLESANVLWPTFISGKWFEKDGRRFVHGTQEKGFQGRIEVRADGQ
                     IDNQALKAKLIHRFESRIDWSLLDMAEPSPTADVVGEGEMRLINGVWQKYAGGRW"
     gene            1244..2953
                     /locus_tag="PN977_005134"
     CDS             1244..2953
                     /locus_tag="PN977_005134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HDI6045046.1"
                     /translation="MADNKITLSSVRKALAGVFKDNGERDNILLSALAVHGGSGYLFS
                     RAGAPVQLSGFLGGKPGDSGMAGDGLVDGSRFIFDEVQLPEDRLQRYPLLEEMAVYST
                     IATALNIHITHALSFDKKTGQTFSIVPVHNGNDSDYDAAQGLCDELMNDIGRTINKEV
                     AGWAFIMSVFGVAYVRPYAKEGIGITSFECSYYTLPGFIKEFEVSGNLAGFSGDYLKD
                     ASGKMVFADPWAIIPMKIPYWRPKSNLMPVHTGHKAYSLLDNPEERTPIETQNYGTSL
                     LEYAYEPYMNLRSAIRSLKATRFNASKIDRIIGLAMNSLDPVKAADYSRTITQTLKRA
                     ADLMEKRARGANNMPTVTNTLLPIMGDGKGQMTIDTQTIQADINGIEDILTYMRQLAA
                     ALGLDYTLLGWADQMSGGLGEGGFLRTAIQAAMRASWIQQGVEEFIQRAIDIHLAFKY
                     GKVYPEGDRPYKIEFHSVNTALQQEHNDNRDSQANYATIVTQILDAVSNNSVLANSDA
                     FKRYLFSDVLEIDEKISEALVNELKAKSEDDDHLMDSIIKTPPQELAQILESVFKEGN
                     END"
     gene            2946..>3837
                     /locus_tag="PN977_005135"
     CDS             2946..>3837
                     /locus_tag="PN977_005135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000132944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head processing protein"
                     /protein_id="HDI6045047.1"
                     /translation="MTDVLKTVTDRFCLYSNARKGRQNGRQYVLSAVKTMLESKETQE
                     GLRLGELFGYYGHGRRQLTGKLEVPETSVIMVEGRPVVIDNVPACRTVAISVDDNGIV
                     THTQEILNTEPGKIVAAMIESRAGGWSWATGGRESGKIAVTTSFHGVDYVTTPNYISL
                     DHPASAGMFESADSKSLLAESLAAHGYSDESVQAVISHYGKMAELEMMVEATERTAEL
                     ETALLESQGRHLEAMAKIADAEARIALLEETAGIRDDVLAAMQDELDNLPIFVSAAQK
                     DAFRLKEPGDAKIVATLFESL"
BASE COUNT          967 a         1026 c          987 g          857 t
ORIGIN      
        1 ccagcgacca ccagcatatt tttgccatac gccattaatc aggcgcattt ccccttcacc
       61 aacaacgtct gcggtcggtg acggttcagc catatcgagc aaagaccagt cgatacggct
      121 ttcgaaacga tgaatcagct tcgctttaag agcctggtta tcaatctgac cgtcggcacg
      181 aacctcaata cgcccctgga agcccttttc ctgggtgcca tgaacaaacc ggcggccgtc
      241 cttttcaaac cacttgccag aaataaacgt tggccaaagc acatttgccg attcaagagt
      301 gccttcatcc accaggggga ttttctcagc catctccgcc ggatgtttgc gcatcagcac
      361 cacatctacg accgtactgg tcccgtttgc gtcaaaagta ccggtaggca agcggtgggc
      421 accaagaaat tcagctttcc gtgataggcg caggcgtaac cgcttcatgt ttgaacctga
      481 aacaatggac ggcggcacaa tcacgcacat gaatccgcct ggctttatct tgtccagcat
      541 gcggagcatg aagtaagaac ccatgtccgt ttcttctgcg taaggcttat cgatgttgcg
      601 tgtgttatca cgaccaccga acggaacgtt acccacaaca tggtcgaatg aatcgttagg
      661 cgtgcttaca gccagttgtt cgaacggaga aatctgtacg ctgtcttccg ggtgcaacag
      721 ctggtttata cgaccggaaa cactgctgat ctcagtcgcg gtcatcaccg taccaaccgg
      781 ttttgtctca ttaaaaacgc cggttcccgc cgatggttcc agagtgttac ctacgtccgc
      841 gccgtagagc ttcatgatct cccagacacc ttcagcgatc ggctttggtg tgtaatattc
      901 ggagacggac ccgccaatgc cgccttcacc ggtgtaccca gccaggatct ggcgctgttc
      961 atctgtcagt gtcgcgccgt ccaccagcga attaagcaaa tctatcgcct tctgattcgc
     1021 ctcacggcgc agtcggtcat agcttttgcc ttccaccttt tccacgccgt atttaatcgg
     1081 cgctcggtga gatgttattg ccctaatgta tttcaatatt tcgctgacac ttgaacagcg
     1141 aaacaccccc atagatagct tgttcattgg taatccttaa caagtgacta gtgttaaatt
     1201 tccgttcaaa cacgatgcga attattctaa ttaaggtgca atcttggcag acaataaaat
     1261 cacgctatcc tcggtcagga aggcgctggc gggggttttt aaagacaacg gagaacggga
     1321 caacatcctc ctgtccgcgc tggctgtgca cggcggaagt gggtatttgt tttctcgcgc
     1381 aggggcaccg gtacaactgt ccggcttctt aggcggcaaa ccgggcgata gtggcatggc
     1441 tggtgatggg ctggtggatg ggagtcgctt tatctttgat gaagttcaac tgccggaaga
     1501 ccgcttgcaa cgctatccgc tactcgaaga aatggcggtt tacagcacga tcgccaccgc
     1561 gctgaacatc catattacgc acgcgctctc tttcgataag aagaccggac aaaccttctc
     1621 tatcgtgccg gtacacaacg gaaacgatag tgactatgac gccgcgcagg ggttgtgtga
     1681 cgagctgatg aacgacatcg ggcgaaccat caacaaagag gtcgccgggt gggcatttat
     1741 catgtctgta tttggggtgg cttatgtcag gccatacgcc aaagaaggca tagggatcac
     1801 gtcttttgag tgctcctatt acacccttcc gggcttcatc aaagagttcg aggtcagcgg
     1861 caacctggcg ggatttagcg gcgattatct gaaggacgcg tcagggaaaa tggttttcgc
     1921 cgatccgtgg gccattatcc ctatgaaaat cccctactgg cggcctaagt caaaccttat
     1981 gcctgtgcac actggccata aagcatacag cttgctggat aatccggaag agcgcacgcc
     2041 gattgaaacc cagaattacg ggaccagctt gctcgaatac gcctacgagc cgtacatgaa
     2101 tctgcgttcg gcgatccgct cgctgaaggc aacgcgtttt aatgcgtcga aaattgaccg
     2161 aatcatcggc ctggcgatga atagtctgga tccggtaaaa gcagccgatt attcgcgcac
     2221 cattactcag acgcttaaac gagcagctga cctgatggaa aagcgcgcac gtggcgcgaa
     2281 taacatgcct acggtgacca ataccctgct gcctattatg ggcgacggca agggacagat
     2341 gactattgat actcagacca tccaggctga catcaacggc attgaagaca ttctcaccta
     2401 tatgcgccag ctggcagcag cacttggcct cgattacacc ctcctggggt gggcagatca
     2461 aatgtccggc gggcttggcg aaggtggatt cctgcgcacg gcaattcagg ccgccatgcg
     2521 cgcctcatgg atccagcagg gcgtagaaga gttcattcag cgggctatcg atattcatct
     2581 tgctttcaag tacggcaagg tatacccgga aggtgatcgc ccgtacaaaa tcgaattcca
     2641 ctccgttaat accgctctgc aacaagagca caacgacaac cgcgactcgc aggcgaacta
     2701 cgccaccatc gttacgcaaa tcctcgatgc cgtcagcaat aacagcgtcc tcgccaattc
     2761 cgatgcattc aaacgttacc tgttcagcga tgtgctggag attgacgaaa aaatctctga
     2821 agcactggtg aacgaactga aagcgaaaag cgaggacgac gatcacctga tggattccat
     2881 catcaaaaca ccgccacagg aactggcgca aatccttgaa tcggtcttta aagagggaaa
     2941 cgagaatgac tgatgttttg aaaacggtca ctgaccgctt ttgtctctat agtaatgcta
     3001 gaaaaggtcg ccagaacggg cgacagtatg tattaagcgc ggtaaagacc atgcttgaaa
     3061 gcaaggaaac tcaggaaggt ttacgccttg gtgagctttt cggctattac ggtcacggtc
     3121 gccgacagct gaccggcaaa ctggaagtac cagaaaccag cgtgatcatg gtggaaggtc
     3181 gcccggtcgt tatcgacaat gttccagctt gccgcacagt agctatatcc gttgacgaca
     3241 acggcatcgt tacccataca caggaaattc ttaacacaga gccgggtaaa attgttgccg
     3301 cgatgatcga aagccgagct ggtggctgga gctgggccac tggcgggcgc gagtccggga
     3361 aaatcgctgt aaccaccagc ttccatggtg tggattatgt gacaacgccg aactatatca
     3421 gtctggatca tcctgccagc gccggaatgt ttgaaagcgc ggattctaaa tctttactgg
     3481 cagagtccct ggcggcgcat gggtactccg acgagtcagt gcaggccgtt atatcccatt
     3541 acggcaaaat ggctgaactg gaaatgatgg tggaggcgac agagcgtacg gcagaactgg
     3601 aaaccgcact actcgaaagc cagggccgcc acctcgaagc aatggccaag atcgcagatg
     3661 ctgaagcgcg aatcgctttg ctggaggaaa cagcgggtat ccgcgacgat gtgctggcag
     3721 caatgcaaga cgaactggat aacctcccga tcttcgtctc cgccgcccaa aaagacgcat
     3781 tccgcctcaa agaacctggt gatgcaaaaa tcgttgccac acttttcgaa tctctga
//
LOCUS       DAMZFT010000263         3725 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.263, whole genome shotgun sequence.
ACCESSION   DAMZFT010000263 DAMZFT010000000
VERSION     DAMZFT010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3725)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3725)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3725
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.263"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..2414
                     /gene="fdnG"
                     /locus_tag="PN977_005136"
     CDS             <1..2414
                     /gene="fdnG"
                     /locus_tag="PN977_005136"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HDI6045048.1"
                     /translation="MTNHWVDIKNANVVMVMGGNAAEAHPVGFRWAMEAKNNNDATLI
                     VVDPRFTRTASVADIYAPIRSGTDITFLSGVLRYLIENNKINAEYVKHYTNASLLVRD
                     DFAFEDGLFSGYDAEKRQYDKSSWNYQFDENGYAKRDETLTHPRCVWNLLKAHVSRYT
                     PDVVENICGTPKADFLKVCEVLASTSAPDRTTTFLYALGWTQHTVGAQNIRTMAMIQL
                     LLGNMGMAGGGVNALRGHSNIQGLTDLGLLSTSLPGYLTLPSEKQVDLQSYLEANTPK
                     ATLADQVNYWSNYPKFFVSLMKSFYGDAAQKENNWGYDWLPKWDQTYDVIKYFNMMDE
                     GKVTGYFCQGFNPVASFPDKNKVVSCLSKLKYMVVIDPLVTETSTFWQNHGESNDVDP
                     ASIQTEVFRLPSTCFAEEDGSIANSGRWLQWHWKGQDAPGEARNDGEILAGIYHHLRE
                     LYQAEGGKGVEPLMKMSWNYKQPHEPQSDEVAKENNGYALEDLYDANGVLIAKKGQLL
                     SSFAHLRDDGTTASSCWIYTGSWTEQGNQMANRDNSDPSGLGNTLGWAWAWPLNRRVL
                     YNRASADINGKPWDPKRMLIQWNGSKWTGNDIPDFGNAAPGTPTGPFIMQPEGMGRLF
                     AINKMAEGPFPEHYEPIETPLGTNPLHPNVVSNPVVRLYEQDALRMGKKEQFPYVGTT
                     YRLTEHFHTWTKHALLNAIAQPEQFVEISETLAAAKGINNGDRVTVSSKRGFIRAVAV
                     VTRRLKPLNVNGQQVETVGIPIHWGFEGVARKGYIANTLTPNVGDANSQTPEYKAFLV
                     NIEKA"
     gene            2427..3311
                     /gene="fdnH"
                     /locus_tag="PN977_005137"
     CDS             2427..3311
                     /gene="fdnH"
                     /locus_tag="PN977_005137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase N subunit beta"
                     /protein_id="HDI6045049.1"
                     /translation="MAMETQDIIKRSATNSITPPSQVRDYKAEVAKLIDVSTCIGCKA
                     CQVACSEWNDIRDEVGHCVGVYDNPADLSAKSWTVMRFSETEQNGKLEWLIRKDGCMH
                     CEDPGCLKACPSAGAIIQYANGIVDFQSENCIGCGYCIAGCPFNIPRLNKEDNRVYKC
                     TLCVDRVSVGQEPACVKTCPTGAIHFGTKKEMLELAEQRVAKLKARGYEHAGVYNPEG
                     VGGTHVMYVLHHADQPELYHGLPKDPKIDTSVNLWKGALKPLAAAGFIATFAGLIFHY
                     IGIGPNKEVDDDEEEHHE"
     gene            3304..>3725
                     /locus_tag="PN977_005138"
     CDS             3304..>3725
                     /locus_tag="PN977_005138"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit gamma"
                     /protein_id="HDI6045050.1"
                     /translation="MSKSKMIVRTKFIDRACHWTVVICFFLVALSGISFFFPTLQWLT
                     QTFGTPQMGRILHPFFGIAIFVALMFMFVRFVHHNIPDKKDIPWLLNIVEVLKGNEHK
                     VADVGKYNAGQKMMFWSIMSMIFVLLVTGVIIWRPYFA"
BASE COUNT          891 a          939 c         1076 g          819 t
ORIGIN      
        1 cgatgaccaa ccactgggtg gatatcaaaa acgctaacgt cgtgatggtg atgggcggta
       61 acgctgctga agcacatccc gtcggtttcc gctgggcgat ggaagcgaaa aacaacaacg
      121 atgcaacctt gatcgttgtc gatccccgtt ttacgcgtac cgcttctgtg gcggatattt
      181 acgcacctat tcgttccggt acggacatta cgttcctgtc tggcgttttg cgctacctga
      241 tcgaaaacaa caaaatcaac gccgaatatg ttaagcatta caccaacgcc agcctgctgg
      301 tgcgtgatga ttttgctttc gaagacggtc tgttcagcgg ctacgacgct gaaaaacggc
      361 aatacgataa atcgtcctgg aactatcagt tcgatgaaaa cggctatgcg aaacgcgatg
      421 aaacactgac tcatccgcgc tgtgtgtgga acctgctgaa agcgcacgtt tcccgctaca
      481 cgccggacgt agtagaaaac atttgcggta cgccaaaagc cgacttcctg aaagtgtgtg
      541 aagtgctggc ctccaccagc gcaccggatc gcacaaccac cttcctgtac gcgctgggct
      601 ggacgcagca caccgtgggt gcgcagaaca tccgtacgat ggcgatgatc cagttgctgc
      661 tcggtaacat gggtatggcc ggtggcggcg tgaacgcatt gcgtggtcac tccaacattc
      721 agggcctgac tgacttaggc ctgctctcta ccagcctgcc aggttatctg acgctaccgt
      781 cagaaaaaca ggttgatttg cagtcgtatc tggaagcgaa tacgccgaaa gcgacgctgg
      841 ctgatcaggt gaactactgg agcaactatc cgaagttctt cgttagcctg atgaaatctt
      901 tctacggcga tgccgcgcag aaagagaaca actggggcta cgactggctg ccgaagtggg
      961 accagaccta cgacgtcatc aagtatttca acatgatgga cgaaggcaaa gtcaccggtt
     1021 atttctgcca gggctttaac ccggttgcgt ccttcccgga caaaaacaaa gtggtgagct
     1081 gcctgagcaa gctgaagtac atggtggtaa tcgatccgct ggtgactgaa acttctacct
     1141 tctggcagaa ccacggcgag tcgaacgatg tcgatccggc gtctattcag actgaagtat
     1201 tccgtctgcc ttcgacctgc tttgctgaag aagatggttc tatcgctaac tccggtcgct
     1261 ggttgcagtg gcactggaaa ggtcaggacg cgccgggcga agcgcgtaac gacggcgaaa
     1321 ttctggcggg tatctaccat catctgcgcg agctgtacca ggccgaaggt ggtaaaggcg
     1381 tagaaccgct gatgaagatg agctggaact acaagcagcc gcacgaaccg caatctgacg
     1441 aagtagctaa agagaacaac ggctacgcgc tggaagatct ctatgatgcc aatggcgtgc
     1501 tgattgcgaa gaaaggtcag ttgctgagta gctttgcgca tctgcgtgat gacggtacaa
     1561 ccgcatcgtc ttgctggatc tacaccggta gctggacaga gcagggcaac cagatggcta
     1621 accgcgataa ctccgaccca tctggtctgg ggaatacgct gggatgggcc tgggcgtggc
     1681 cgctcaaccg tcgcgtactc tacaaccgtg cttcggcgga tatcaacggt aaaccgtggg
     1741 atccgaaacg gatgctgatc cagtggaacg gcagcaagtg gacgggtaac gatattccgg
     1801 acttcggcaa tgccgcgcca ggtacaccaa ccggaccgtt tatcatgcag ccggaaggga
     1861 tgggacgcct gtttgccatc aacaaaatgg cggaaggtcc gttcccggaa cactacgagc
     1921 caatcgaaac gccgctgggc actaacccgc tgcatccgaa cgtggtgtct aacccggtcg
     1981 ttcgtctgta tgaacaagac gcgctgcgga tgggtaaaaa agagcagttc ccgtatgtgg
     2041 gtacgaccta tcgtctgacc gagcacttcc acacatggac caagcacgcg ttgctcaacg
     2101 caattgctca gccggaacag tttgtggaaa tcagcgaaac gctggcggcg gcgaaaggca
     2161 ttaataatgg cgatcgtgtc actgtctcaa gcaagcgtgg ctttatccgc gcggtggcgg
     2221 tggtaacgcg ccgtctgaaa ccgctgaatg taaacggtca gcaggttgaa acggtgggta
     2281 ttccgatcca ctggggcttt gagggtgtcg cgcgtaaagg ttatatcgct aacactctga
     2341 cgccgaatgt cggtgatgca aactcgcaaa cgccggaata taaagcgttc ttagtcaaca
     2401 tcgagaaggc gtaagggggc gaacagatgg ctatggaaac gcaggacatt atcaaaaggt
     2461 ccgcaactaa ctccatcacg ccgccttctc aggtgcgtga ttacaaagca gaagtcgcga
     2521 aacttatcga cgtttccacc tgtatcggtt gtaaagcctg tcaggtggcg tgttcggagt
     2581 ggaacgacat ccgtgatgaa gtggggcact gcgtcggggt ttacgataac cccgccgatc
     2641 tgagcgccaa gtcctggact gtgatgcgct ttagcgaaac cgaacagaac ggcaagctgg
     2701 agtggctgat ccgtaaagac ggctgtatgc actgtgaaga tccgggctgc ctgaaggcgt
     2761 gcccgtctgc tggtgcaatc attcagtacg ctaacgggat tgtcgatttc cagtcggaaa
     2821 actgcatcgg ctgtggttac tgcattgccg ggtgtccgtt taatattccg cgcctcaaca
     2881 aagaggataa ccgggtatat aaatgcacgc tctgcgtcga tcgcgtcagc gtcggccagg
     2941 aaccggcttg tgtgaaaacc tgtccgaccg gggctatcca cttcggcacc aagaaggaga
     3001 tgctggagct ggcggaacag cgcgtggcga aactgaaagc gcgtggttac gaacatgctg
     3061 gcgtctacaa cccggaaggg gtcggtggta cgcacgtcat gtacgtgttg catcacgccg
     3121 atcagccgga gctgtatcac ggtctgccga aagatccgaa gatcgacaca tcggtgaact
     3181 tgtggaaagg cgcgttgaaa ccgctggcag cggctggctt tatcgccact tttgccgggt
     3241 tgattttcca ctacatcggt attggcccga ataaggaagt ggacgatgac gaggaggagc
     3301 atcatgagta agtcgaaaat gattgtgcgc accaagttta ttgatcgcgc ctgtcactgg
     3361 accgtggtga tttgcttctt cctggtggcg ctgtccggga tttcgttctt cttcccgacg
     3421 ctgcaatggc tgacgcaaac cttcggtacg ccgcagatgg gacgcatttt gcacccgttc
     3481 ttcggcattg cgattttcgt cgcgctgatg tttatgtttg tgcgttttgt gcatcacaac
     3541 atcccggata agaaagatat tccgtggctg ttgaacattg tcgaagtatt aaaaggcaat
     3601 gagcacaaag tggcggatgt tggtaagtac aacgccgggc agaagatgat gttctggtcg
     3661 atcatgagca tgattttcgt gctgctggtg accggggtga ttatctggcg tccgtacttt
     3721 gcgca
//
LOCUS       DAMZFT010000264         3656 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.264, whole genome shotgun sequence.
ACCESSION   DAMZFT010000264 DAMZFT010000000
VERSION     DAMZFT010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3656)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3656)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3656
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.264"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            484..678
                     /locus_tag="PN977_005139"
     CDS             484..678
                     /locus_tag="PN977_005139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="HDI6045051.1"
                     /translation="MNKQQQTALNMARFIKSQSLTLLEKLDALDADEQAAMCERLHEL
                     AEELQNSIQVRFEAESETGT"
     gene            662..895
                     /locus_tag="PN977_005140"
     CDS             662..895
                     /locus_tag="PN977_005140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6045052.1"
                     /translation="MKQEHNEADGGGHGFPVGVFVSRQARQGASGGFSGCRGAAMTQS
                     RSDSGVYTGLTMQHQCELYGKRTMSGVKCRTDA"
     gene            1342..1500
                     /locus_tag="PN977_005141"
     CDS             1342..1500
                     /locus_tag="PN977_005141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HDI6045053.1"
                     /translation="MHEPPVQPDRCALSGNYRLESNPVRHDKAPLAAATGNWTRWIEI
                     LRVLEVVT"
     gene            1979..2860
                     /locus_tag="PN977_005142"
     CDS             1979..2860
                     /locus_tag="PN977_005142"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6045054.1"
                     /translation="MLRSNIIDAASSVEDLIAKLGGTGRGLREKTNSISHLLEPSYVK
                     KINMIATVRNKAAHERVPPTNIQDFERVISEVKDYLTELYYKKIASKTYSQSNTLFDI
                     IDAAISVEDLITKLGGTGRGLREKTNSISHLLEPSYVKKINMIATVRNKAAHERVLPT
                     NIQDFERAISEVKDYLTELYYKKIASKTHSQSNTRFENLSKEELREELARLVDEEISR
                     NKAELEYMRSGSFIKDKITKEAETKQDTTEQQKTFKQQPNGWSSLSTTGKIISATGIA
                     IGCAVLGIFGNPFDKRN"
     gene            2844..3362
                     /locus_tag="PN977_005143"
     CDS             2844..3362
                     /locus_tag="PN977_005143"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6045055.1"
                     /translation="MIKEIETDKPSKVIFYRSSIVKQYSKGVRMDWYDVKWFFRDVCD
                     SVSNVAGNIYDVAADGVGAVTDFIEENPKTSIAIGTVVGIATAGVAAPAIAASIGATG
                     VLGTTAGGTAISSLSGAALSSASLAALGGGSIAAGGAGMAGGTCVVAAGGGIAAGTVS
                     LGGVAATKTIKS"
BASE COUNT         1085 a          872 c          883 g          816 t
ORIGIN      
        1 aaaaaaacac accgcgccgc tagcagcacc cctaatataa gatgatgttt tttatgaaaa
       61 tagtcagtac cacccctaaa aagcggtgtt ggcgcagcta caacaccgcg ccgacaccgc
      121 ttttttaaat atcacaaaga gagcaagaag aactaatttg tcataactct ctatttataa
      181 agaaaaatca gcaaaaactt gtttttgcgt ggggtgtggg catttcaggc atgagaacag
      241 actcgcgttt ttccggagta tcagcggaat tattgattaa gaaacactcg acgcagagcg
      301 aaattttatg caacagcggc tgcactgcac agggattatt tatcagttga tatgctgtgc
      361 catgatacgt cctaaaaaaa caggacagga tgtgcaattg taataccgtc gcccgcaacg
      421 tcttacaatt accacctggt cagggtccac ccgacacccg gtactgacgg agaacaacgg
      481 aaggtgaaca aacagcaaca aactgcgctg aatatggcgc gatttatcaa aagccagagc
      541 ctgacactgc ttgaaaaact ggatgctctg gatgccgacg aacaggcggc catgtgcgaa
      601 cgactgcacg aactcgcgga agaactccag aacagcatcc aggttcgctt tgaggccgaa
      661 agtgaaacag gaacataacg aagctgacgg aggcggtcac ggctttcccg tgggcgtttt
      721 cgtcagcaga caagcccgtc agggcgcgtc agggggtttt agcgggtgtc ggggcgcagc
      781 catgacccag tcacgtagcg atagcggagt gtatactggc ttaactatgc agcatcagtg
      841 cgaattgtat ggaaagcgca ccatgtccgg tgtgaaatgc cgcacagatg cgtaaggaga
      901 aaatgcacgt tccggcgctc ttccgcttcc tcgctcactg actcgctacg ctcggtcgtt
      961 cggctgcggc gagcggtgtc tgctcactca aaagcggaga tgcggttatc cacagaatca
     1021 ggggataaag ccaaaagaaa catgtgagca aaaaacgaga acccggaaaa ggctgcgccg
     1081 ttggcgtttt tccataggct ccgcccccct gacgagcatc ataaaaatcg acgctcaagt
     1141 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc
     1201 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct
     1261 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcggctc ggtgtaggtc
     1321 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta
     1381 tccggtaact atcgtcttga gtccaacccg gtaagacacg acaaagcgcc actggcagca
     1441 gccactggta actggacaag gtggattgag atattaagag ttcttgaagt ggtgacctaa
     1501 ctgcggctac actagaagga cagtatttgg tatctgtgct ccaccaagcc agttaccttc
     1561 ggaagaaaga gttggtagct ctagattccg gcaaacaaac caccgttggt agcggtggtt
     1621 tttttgttta caggcagcag attacgcgca gaaaaaaagg atcacaagag gatcctttga
     1681 tcttttcacg ccacgtcctt agtcatagcc atagctcaca atttttaacc acaattgact
     1741 ccaagccgga aacccagaaa atacctccac caaagcaagt aatcgacaga tttgcatgac
     1801 gttgaatagg tgacaggtca tgtgtcgaaa tcgttgtctc ggtgacggtc attcaatcag
     1861 ttgtttgtgc cccccctccc atcaaacaag tcgcgaaaaa tccaagcctc cggcatcagc
     1921 acataatttt gacgcactca aaaatacggt atccttttag gaatatggag gatcagaaat
     1981 gctacgttct aacatcattg atgcagcaag ttccgtggaa gacctgatag ccaaattagg
     2041 cggtacagga cgtggactac gtgaaaaaac caacagtata agccacctgc ttgaaccaag
     2101 ttacgtaaaa aaaatcaaca tgatagcgac ggttcgtaac aaagctgcgc acgaacgagt
     2161 cccccctaca aacattcagg attttgaacg tgtcatatca gaagtaaaag actatttaac
     2221 cgaactttat tataaaaaaa tcgcgtcaaa gacatattct caatcaaata ctcttttcga
     2281 catcattgat gctgcaattt ccgtggaaga cctgataacc aaattaggcg gtacggggcg
     2341 tgggctacgt gaaaaaacca acagtataag ccacctgctt gaaccaagtt acgtaaaaaa
     2401 aatcaacatg atagcgacag ttcgtaacaa agccgcgcac gaacgagtcc tccctacaaa
     2461 tattcaggat tttgaacgtg ccatatcaga agtaaaagac tatttaaccg aactttatta
     2521 taaaaaaatc gcatcaaaga cacattctca atcaaatact cgtttcgaaa acctcagcaa
     2581 agaagagctt cgagaagagc tggcgaggct tgtcgatgag gaaatatcca gaaataaagc
     2641 tgagctggaa tacatgcgga gcggttcgtt tatcaaggat aaaataacca aagaagcaga
     2701 aacaaaacag gatactaccg aacaacagaa gacttttaag cagcaaccaa acgggtggtc
     2761 aagtttgagc acgacaggga aaatcatttc tgccaccggg atcgccattg gttgtgcagt
     2821 gctcggtatc tttggtaatc catttgataa aagaaattga gactgacaaa ccttctaagg
     2881 ttatttttta tcgttcatct atcgtcaagc aatattcaaa aggagtgaga atggattggt
     2941 acgatgtaaa atggtttttc cgcgatgttt gcgactccgt ttcaaatgtg gcaggtaata
     3001 tttacgatgt ggcagctgac ggcgtagggg ctgttacaga ttttatcgaa gagaacccta
     3061 aaacatccat tgcaatagga actgtggttg ggattgcaac agcaggggta gcagctccag
     3121 caatagcagc aagcattgga gcaacaggcg ttttaggaac aactgccgga ggtacggcta
     3181 tatcttcctt aagtggagca gcgctatcca gcgcatcttt agccgcctta ggagggggtt
     3241 caatcgcagc aggaggagcg ggtatggctg gcggaacctg tgtggttgca gcaggaggag
     3301 gcattgcagc agggaccgtt agtctaggag gggttgcagc gacaaaaacg ataaaaagct
     3361 aataagccag caccccattt accataacgt cgcattacgc gcaccgctaa aacgcgctga
     3421 acgcgattct gccgcaaaca ccgcaaaaag agatgtttca cccagtgaaa accggaaaaa
     3481 tgcgtcagga tcgctttcgg ctcactgcat agctatgcat gaaagtgaat ggcgatcggt
     3541 ttgggcgcat acagtgccca tgagacacat tttcggacat tttcagttcg tgaatccctg
     3601 actcattgct acatatctga acagacaccc ctagcagcgc cccaggggtg tcctgt
//
LOCUS       DAMZFT010000265         3636 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.265, whole genome shotgun sequence.
ACCESSION   DAMZFT010000265 DAMZFT010000000
VERSION     DAMZFT010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3636)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3636)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3636
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.265"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..440
                     /locus_tag="PN977_005144"
     CDS             <1..440
                     /locus_tag="PN977_005144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GTPase family protein"
                     /protein_id="HDI6045056.1"
                     /translation="RVLFVVMQADKTEPCHEWDMAGIQPSPAQAQNIREKTEAVFRLF
                     RPVHRVVAVSARTGWELDTLVSALMTALPDHAASPLMTRLQDELRTESVRAQAREQFT
                     GAVDRIFDTAESACIASVARTVLRAVRDSVVSVARAVWNWIFF"
     gene            658..3054
                     /locus_tag="PN977_005145"
     CDS             658..3054
                     /locus_tag="PN977_005145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dynamin family protein"
                     /protein_id="HDI6045057.1"
                     /translation="MHEKNIALLCDEADRLLQLNINLLRQMVEEPDVLSDSKNENRLL
                     FDKQKALKRIEELEGEQIKTARREMVLAVVGTMKAGKSTTINAIVGQEILPNRNRPMT
                     SVPTLIRHVPGKTEPVLHLEHIQPVRNLLITLQEKLATPAGQQVAQTLQQTGDTRELL
                     DILTDDGWLKNEYHGEEEIFTGLASLNDLVRLAAAMGTEFPFDEYAEVQKLPVIDVEF
                     SHLVGMDACQGTLTLLDTPGPNEAGQPQMEVMMRDQLQKASAVLAVMDYTQMNSKADE
                     DVRKELNAIADVSAGRLFVLVNKFDEKDRNGDGADAVRQKVPAMLNSDVLPASRVYPG
                     SSRQAYLANRALHELRKNGTLPVDEAWVDDFIRGAFGCMKKEYVCKDSELATEGATDL
                     WEGSLIDQLITEVIQSSHSRAAALAVDSAAAKLMQNAENISEYLLLRHQGLQQSIQSL
                     QSHITSLLADIREIADCQEQMTTDVRMAMEEIDTKTRELLTGVCTSLEEELNDYFRSG
                     KRKEQQMLEEEDAEQRRSQSGLWGKISQWSGINNQGREDYRKRDFAPDSPEIKFSDRR
                     EALELMTQIESTVTSLHREAEAQFRPELEKIVSGIETGFRGTALYATENIAGRINARL
                     EDEGFTVKISFPAVSQLQTRLAVKINLSALMEERTETVTRRRRQSGVWGTVCRWFGTS
                     DLGWENYDEDVSRSVININKVREEVMSLTRAYFGELQASIEQDINQPVRQEIDAFFCA
                     FREKVEQLRNTLIQSSEDHKRDQQAQERLTGRLQALNERVPELITDSKALREELETML
                     "
     gene            3051..>3636
                     /locus_tag="PN977_005146"
     CDS             3051..>3636
                     /locus_tag="PN977_005146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein"
                     /protein_id="HDI6045058.1"
                     /translation="MTSPFIQQIADNRVCQVLTCLPEKFVVDFANGIDVAQEHIRTAG
                     ERTFFRRLKEGLTGEGAARQNAINASLAQGVEASLRWLTEMTTSLATTNYAITRVNDR
                     VSSLVSDTARLAHYSADTREQLLTLADQVHHKLNHLEEKLHRVDQVQRAQLHLEQIFS
                     WWSAGRYASFSPAGRCYVALEELRWGAFGDVIRQS"
BASE COUNT          941 a          901 c         1014 g          780 t
ORIGIN      
        1 agcgggtact gtttgtggtg atgcaggccg ataaaacgga gccctgccat gaatgggata
       61 tggccggtat tcagccttct cccgcacagg cacagaacat tcgcgaaaaa acggaggcgg
      121 tattccgtct gttccggccc gtacatcggg ttgttgccgt atcggcccgc accggctggg
      181 aactggatac gctggtcagt gcgctcatga cagcacttcc cgaccatgcc gccagtcccc
      241 tgatgacccg cctgcaggac gagctgcgca cggaatctgt ccgcgctcag gcccgtgaac
      301 agtttaccgg tgcggtggac cggatatttg acacggcgga gagcgcctgt attgcctctg
      361 ttgcacgcac ggttctgcgt gccgtccgtg actcggtggt ctctgttgcc cgcgcggtat
      421 ggaactggat tttcttctga acccggtggt gatgtaccag gctgttgtta cagattttta
      481 actccatgaa aacactatgc aaacgcagcc atacccgggt tgtccccttg cgttgtgttt
      541 ctcaagttaa tagaatacat cgatcgttac tatcatatcg atcgttataa acgatcgttt
      601 gtagtaaatg acctcctccg tggttgtcat gaaaatgcgt cttaggaagg ttaaataatg
      661 cacgaaaaga acatcgccct gctttgtgat gaagccgacc gacttttgca actgaacatt
      721 aatctgctcc ggcaaatggt tgaggagcca gatgtgttat ctgacagtaa gaacgaaaac
      781 agactgcttt ttgataaaca gaaagcactg aaaagaattg aggagctgga gggcgaacaa
      841 atcaaaaccg cccgcaggga gatggtgctg gctgttgtcg gcacgatgaa agcaggcaaa
      901 tcaaccacca tcaacgccat tgtggggcag gaaattctgc ctaaccgtaa ccgccccatg
      961 acctctgtac cgacgctcat ccgccacgtt cccggaaaaa ctgagccggt tctccatctg
     1021 gaacatattc agcctgtccg caatttatta atcacactgc aggaaaaact cgccaccccg
     1081 gcaggacagc aggtcgcaca gaccctgcag caaaccgggg atacccgcga actgctggat
     1141 attctgacgg atgatggctg gctcaaaaat gaataccacg gggaggagga aatctttacc
     1201 ggactggcat cgttaaacga tctggttcgt cttgctgcgg caatggggac tgaatttcct
     1261 tttgatgaat acgcagaagt gcagaaactg ccggtgatcg acgtggaatt cagccatctg
     1321 gtggggatgg atgcatgcca gggaacactc acactgctgg atacccccgg ccctaatgag
     1381 gccggacaac cgcagatgga agtgatgatg cgggatcaac tgcagaaagc ctctgcggtt
     1441 ctggctgtga tggattacac ccagatgaac tcaaaagcgg atgaagacgt ccgtaaagag
     1501 cttaatgcca ttgctgacgt atcagccggc cgcctgtttg tactggtcaa taaatttgat
     1561 gagaaagacc gcaatggcga tggggcagat gccgtacgcc agaaagttcc ggcaatgctg
     1621 aacagcgatg tgctgcccgc ctcccgcgtt tatcccggat cctcacgcca ggcatacctg
     1681 gctaaccgtg cgcttcatga gttacggaaa aacggaaccc ttcctgttga tgaagcctgg
     1741 gtcgatgatt ttatcagagg tgccttcggt tgcatgaaaa aagaatacgt ctgtaaagac
     1801 agtgaactgg caactgaagg ggcgacagac ctgtgggaag gctcacttat cgatcaactg
     1861 ataacggaag tcatacagag ctcacattcc agagctgcgg cactggcggt tgactctgcc
     1921 gccgcaaaac tgatgcagaa tgcagaaaat atcagtgaat acctgttgtt acgccatcag
     1981 gggctacagc aaagcattca gtcactgcag tcgcatatca ccagcctgct tgcggatatc
     2041 cgggaaatcg cggactgtca ggagcagatg accactgatg tcagaatggc catggaggag
     2101 atcgatacta aaacccggga attactgacg ggggtctgca cctcactgga agaggagctg
     2161 aatgactatt tcagaagcgg taaacgcaaa gaacagcaaa tgctggagga agaagacgca
     2221 gaacaacgaa ggtctcagtc cggattatgg gggaaaattt ctcaatggtc cggtatcaac
     2281 aaccagggcc gggaggatta caggaaacga gattttgccc cggacagccc cgaaataaaa
     2341 ttcagtgatc gcagggaagc ccttgaactg atgacgcaaa tcgaatcgac cgtgaccagc
     2401 ctgcaccgtg aggctgaagc acagttccgg cctgagctgg agaaaatcgt cagcgggatt
     2461 gaaacaggtt ttcgtggcac ggccctgtac gccacagaaa acattgccgg tcgcatcaat
     2521 gcccgcctgg aggatgaggg ctttaccgta aaaatcagtt ttccggcagt cagccagtta
     2581 cagacccggc tcgcggtaaa aataaatctg agtgcgctta tggaggaaag aacggagacc
     2641 gtcacccgtc gccgtcggca gagtggcgta tggggaaccg tttgtcgatg gtttggcacc
     2701 agtgacctgg gctgggaaaa ctatgacgag gatgtgagtc gcagcgtgat caatatcaac
     2761 aaggtcagag aggaagttat gtcactgacc cgggcatatt tcggggagct gcaggcatcc
     2821 attgagcagg atattaacca gcccgtccgc caggaaatcg atgccttttt ctgcgcattc
     2881 agggagaaag ttgaacaact gcgtaacacg ctgattcaga gctctgaaga tcataaacgc
     2941 gatcagcagg cgcaggaacg gcttaccggg cgacttcagg cattaaatga aagggttcct
     3001 gagctcatta ctgacagtaa ggcgctgagg gaagagctgg agacaatgct gtgacttcac
     3061 catttattca acaaattgct gataaccggg tatgtcaggt actcacctgt cttcctgaaa
     3121 aatttgtggt tgatttcgca aacggaatcg atgttgcaca ggagcatatc cgcacggccg
     3181 gagagcgcac gttcttccgg cgtttaaaag aggggctgac cggcgaaggc gctgcacggc
     3241 agaatgccat caatgcgtcg cttgcacagg gcgttgaggc gtccctgcgc tggctgacgg
     3301 agatgacaac gtcacttgcc accacaaact acgcgattac ccgggtaaac gacagggtca
     3361 gttcactggt cagtgataca gccaggctgg cgcattattc cgcagatacg cgggagcagt
     3421 tactcaccct ggccgatcag gttcaccata aactgaatca tcttgaagaa aaactccacc
     3481 gtgttgacca ggttcagcgg gcgcagttac atcttgagca gatattctca tggtggagcg
     3541 ccgggcgata tgcgtcattt tcccctgccg gacgttgtta tgtggcactt gaagagcttc
     3601 gctggggcgc gtttggtgat gtgatacgtc agagcg
//
LOCUS       DAMZFT010000266         3607 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.266, whole genome shotgun sequence.
ACCESSION   DAMZFT010000266 DAMZFT010000000
VERSION     DAMZFT010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3607)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3607)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3607
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.266"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            92..789
                     /locus_tag="PN977_005147"
                     /pseudo
     CDS             92..789
                     /locus_tag="PN977_005147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612091.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            1462..2970
                     /gene="ltrA"
                     /locus_tag="PN977_005148"
                     /pseudo
     CDS             1462..2970
                     /gene="ltrA"
                     /locus_tag="PN977_005148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708749.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
     gene            3051..3512
                     /locus_tag="PN977_005149"
                     /pseudo
     CDS             3051..3512
                     /locus_tag="PN977_005149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612091.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          903 a          912 c         1037 g          755 t
ORIGIN      
        1 tccacaggct cctgtgcaaa agggctgact gatcttaccc agcaatagtg gacacaggac
       61 taagtgagta aactcttaac cagggtgact catgacaaaa ccagtatcaa tcagcaagaa
      121 gccccgtgaa caacattcgc ctgaatttcg taacgaagcc ctgaaactcg ctgaacgcat
      181 cggtgtggcc gccacagccc gtgaactcag cctgtatgaa tctcagcttt atgcctggcg
      241 cagtaaacag cagcaacaaa tgagttcgtc agagcgcgaa agcgaactgg ccgctgaaaa
      301 tgtccgcctt aaacgacaac tggcggagca ggctgaggaa ctggccatcc tccaaaaggc
      361 cgcgacatac ttcgcgaagc gcctgaaatg aagtatgtct tcatcgaaaa tcatcgggca
      421 gagttcagca tcaaagcgat gtgtcgtgta cttcgggttg cccgcagcgg ctggtatgtc
      481 tggctcaggc gtcgtcacca gatgagcctg cgccaacagt ttcggctcac ctgcgatgcc
      541 gctgtccata aggcattctt tgaggcaaag cagcgatacg gtgctccccg ccttgctgac
      601 gaactgccgg agttcaatat taaaaccatt gccgccagcc tgcgtcgtca ggggctgcgg
      661 gcgaaagcca gccggaagtt cagcccggcc agctaccgtg cacatggcct gcccgtattg
      721 gagaatctgc tggagcagga cttcagcgcc agcggcccga accagaagtg ggtgggtgac
      781 atcacgtact gtgtgcccgg agttcagggc gagcatagac gctcaaatga accacgagtc
      841 tgtctggaat attgaaccgg taactcacga tgagaaaccc aacaatccca ccgggtgtga
      901 cggtggagaa cctgagcggc agtgacctgc ggcatgcccg cagggtgatg taacccgctg
      961 acaacgggga ttgaggcgag atcactaagc cgagatgatc ctcaaggtta agtactgaaa
     1021 ggcttaagaa tatgaacccg ttaatccgcc tctgtgggtt gaaaacgtca ccacggccta
     1081 cgtgatctga caggccgtgc aggaggaact ggcagtgata cgtaagcact gccggtcgaa
     1141 ggtgttttga catgtatgcg aaactccggg gcagcagcgt ctatcacgct cgcgttgctg
     1201 acttctgcca acttgcggca agcaaggata aagagtgcga cgggcagcct cctcagtatg
     1261 tctgagtcca gtcaggcaaa ccggggaagg tcagcgacgg atgttaaggg ggcatggctc
     1321 cgatgacgcg ctggctggcg gagcttccgt agtagtccgc gatggggaaa gcccattaca
     1381 tggcgaaggg aagcagtttg aatgtgtctg cgacgtaaat taactgacct aacgaggtga
     1441 agacctttga taatcagcga aatgcaacgc aagcttgcca catgggcagc caccgatccg
     1501 tccctacgga ttcaacggct gctgcgtctg ataacacaac cagaatggct ggctgaagcg
     1561 gcgcggatca cgctttcatc aaagggggcg aatacccccg gtgttgatgg cgtgaacaaa
     1621 acaatgctac aggccagact ggctgttgag ctgcaaatcc tcagggatga attactctca
     1681 ggccactacc agcccttgcc cgccaggcgg gtttacatcc ctaaaagcaa tggcaaactg
     1741 cgaccactgg gtatccccgc gttgcgggat cgtattgttc agcgggccat gctgatggcg
     1801 atggagccga tatgggagag tgattttcat acgctctcat atggcttccg gcctgagcgc
     1861 agtgtccacc actcgatccg cacggtgaaa ttacagctca ccgactgcgg tgaaacccgg
     1921 ggacgctggg tgattgaagg cgacctgtcc agttacttcg acaccgtaca tcatcgactg
     1981 ctgatgaagg ctgtacgccg caggatcagt gacgcacgtt tcatgactct gctgtggaaa
     2041 accatcaagg cgggacatat cgatgtcggt ctctttcggg cggccagtga aggtgtgcca
     2101 cagggcggtg ttatatcgcc gctattgtcg aacatcatgc tgaatgagtt cgatcaatac
     2161 ctgcatgagc gctacctgag cgggaaagca agaaaagatc ggtggtactg gaataacagt
     2221 atccaacggg gccgaagtac ggcggtcaga gaaaactggc agtggaaacc cgcggtggcg
     2281 tactgccgct atgccgatga ttttgtcctc atcgtcaaag gcaccaaagc acaggcggaa
     2341 gccatcaggg aggagtgtcg gggtgtgctc gaaggcagtc tgaaactcag gctgaacatg
     2401 gataagacta aaatcaccca tgttaatgac ggctttatct ttctggggca caggatcatt
     2461 cgcaaacgca gtcgttatgg cgagatgcaa gtggtctcaa cgatcccgct ggagaaagcc
     2521 agaaacttcg ccgcatcgct gacagcactg ttatcaggca actacagtga aagcaaagtc
     2581 gatatggctg aacaactcaa ccgaaaactg aaaggctggg ccatgttcta tcagttcgtt
     2641 gattttaagg ccaaagtctt cagttatatc gaccgtgtcg tgttctagaa gctggctcac
     2701 tggctggccc gcaaataccg tacaagtatc gcttccctga tgaggtggtg gtgtaaatca
     2761 ccgaaaccgg gtcagagcaa aacgtgggtt ttatttggta aaaccaatca cggcaagctc
     2821 agcggcgaaa tactgtaccg gttggtgggg caaggcaaga agctgttccg ctggcggcta
     2881 cccgaaggta atccctatct gaggacggaa accagaaaga cgtatacatc gcgctttaca
     2941 gaagtggcaa tggcgttcgc cagcgtttaa atggagagcc ggatgcgctg aaaggtgcat
     3001 gtccggttcg gggaggagag gcagggaaat agtccgacta cgccctgcct cttactctac
     3061 tgcgtaccga tgagggctgg ttgtatctca cagtagtcat cgacctatgg tcacgcgccg
     3121 tcattggctg gtcgatgtca ccgcgaatga cagcacaact ggcctgtgat gcacggcaaa
     3181 tggcgttgtg gcggagaaga cgcccggaaa gcgtcattgc tcatacggac cgtggtggtc
     3241 aatactgttc aggggattat caggcgctgc tgaagcgaca caacctgcgt ggcagtatga
     3301 gtgcgaaagg ctactgttat gacaatgcct gtgtggaaag cttctttcat tcgctgaagg
     3361 tggaatgtat ccacggggaa cgctttagca gccgggaaat aatgcgggca acggtgttta
     3421 attatatcga gtgtgattac aatcgctggc gtcgtcacag tgcctgtggc ggtctcagcc
     3481 cggaacaatt tgaaaaccat aatctcgctt agggccgtgt ccacattacg tgggtaggat
     3541 cactccaccg tcacacccga tgggattgtt gggtttctca tcgttagcta ccgattctat
     3601 attccag
//
LOCUS       DAMZFT010000267         3496 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.267, whole genome shotgun sequence.
ACCESSION   DAMZFT010000267 DAMZFT010000000
VERSION     DAMZFT010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3496)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3496)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3496
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.267"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(<1..1001)
                     /locus_tag="PN977_005150"
     CDS             complement(<1..1001)
                     /locus_tag="PN977_005150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FHIPEP family type III secretion protein"
                     /protein_id="HDI6045059.1"
                     /translation="MSNLAAMLRLPANLKSTQWQILAGPILILLILSMMVLPLPAFIL
                     DLLFTFNIALSIMVLLVAMFTQRTLEFAAFPTILLFTTLLRLALNVASTRIILMEGHT
                     GAAAAGKVVEAFGHFLVGGNFAIGIVVFVILVIINFMVITKGAGRIAEVGARFVLDGM
                     PGKQMAIDADLNAGLIGEDEAKKRRSEVTQEADFYGSMDGASKFVRGDAIAGILIMVI
                     NVVGGLLVGVLQHGMSMGHAAESYTLLTIGDGLVAQIPALVISTAAGVIVTRVSTDQD
                     VGEQMVNQLFSNPSVMLLSAAVLGLLGLVPGMPNLVFLLFTAGLLGLAWWIRGREQKA
                     "
     gene            complement(994..2142)
                     /gene="flhB"
                     /locus_tag="PN977_005151"
     CDS             complement(994..2142)
                     /gene="flhB"
                     /locus_tag="PN977_005151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FlhB"
                     /protein_id="HDI6045060.1"
                     /translation="MSDESDDKTEAPTPHRLEKAREEGQIPRSRELTSLLILLVGVCV
                     IWFGGVSLARRLSGMLSAGLHFDHSIINDPNLILGQIILLIREAMLALLPLISGVVLV
                     AIISPVMLGGLVFSGKSLQPKFSKLNPLPGIKRMFSAQTGAELLKAILKTILVGSVTG
                     FFLWHHWPQMMRLMAESPITAMGNAMDLVGLCALLVVLGVIPMVGFDVFFQIFSHLKK
                     LRMSRQDIRDEFKQSEGDPHVKGRIRQMQRAAARRRMMADVPKADVIVNNPTHYSVAL
                     QYDENKMSAPKVVAKGAGLVALRIREIGAENNVPTLEAPPLARALYRHAEIGQQIPGQ
                     LYAAVAEVLAWVWQLKRWRLAGGQRPVQPTHLPVPEALDFINEKPSHE"
     gene            2379..>3496
                     /gene="dgt"
                     /locus_tag="PN977_005152"
     CDS             2379..>3496
                     /gene="dgt"
                     /locus_tag="PN977_005152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012311981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dNTP triphosphohydrolase"
                     /protein_id="HDI6045061.1"
                     /translation="MEWQQLLNCNRRKDNLKKTSIEFLHPLASGRQEIERDYDRLLFA
                     APTRRLADKTQVFPLDQNDSIRTRLTHSHEVANFARGIGMRLAFEMKEHIFGEIPTGI
                     VVERDVPALLAAIGLAHDLGNPPFGHQGEAAMRAWFNKNLPGLLDKEVNSEIYNDFLQ
                     FDGNSQTLRLVTKLQIINDNFGLNLTYATLAALIKYPRASYSSDKHWKKHGFFYSEHE
                     VVMDVWQQTGLGVPGNDAGCRHPFTWIMEACDDIAYSVLDAEDTIKKGFASYQDLRDF
                     LMSQSEPNDVIQHVIEAVEAKNDAWKEQRNALSPAEINELSMQMFRVYATSALINAVV
                     EAFREQLPTLMQRDCPFKDLISQSKGHALCTALKMFDRT"
BASE COUNT          942 a          943 c          831 g          780 t
ORIGIN      
        1 gctttttgtt cacgtccacg gatccaccag gccagcccca gcaatccggc agtgaacagc
       61 aaaaatacca ggtttggcat tccaggcacc aggccgagta aaccgagcac ggcggcgctt
      121 aacaacataa cgcttgggtt actgaaaagc tgattcacca tctgctcgcc aacatcctga
      181 tcggtgctga cacgcgtaac gatgaccccc gcggcggtag aaatcaccaa cgccggaatt
      241 tgtgccacca gaccgtcgcc aatggtcaat agcgtataac tttccgccgc gtgtcccatg
      301 ctcatgccat gttgcagcac gccgaccagc aacccgccga caacgttaat gaccatgatg
      361 aggatcccgg cgatggcatc gccgcgaaca aacttacttg ccccatccat tgagccgtaa
      421 aaatcggctt cctgagtcac ttcggagcgg cgttttttcg cctcatcttc accaatcaat
      481 ccagcgttaa ggtcggcgtc aatcgccatc tgcttacccg gcatgccatc gagaacaaag
      541 cgcgcaccca cttctgcgat acgcccggca cctttggtaa tgaccataaa gttgatgatc
      601 acgagaatga caaacaccac gataccgata gcgaaattgc caccaacgag gaagtgaccg
      661 aacgcttcga ccacctttcc agccgccgcc gcgccggtat gcccttccat caaaatgata
      721 cgggttgaag ccacgttaag tgccagacgc aacagcgtgg taaacaacag gatggtcgga
      781 aacgcagcaa actcaagcgt gcgctgggta aacatcgcca ccagcaacac catgatcgac
      841 aaggcaatat tgaaggtaaa cagcaggtcg agtatgaatg cgggcagtgg cagtaccatc
      901 atcgacaaga tcaacaggat caaaatcggt ccggcaagga tctgccattg tgtcgatttc
      961 aggtttgcgg gcaggcgcag catcgcggcc agattactca tgggacggtt tctcgttaat
     1021 aaaatccagg gcttccggca ccggaagatg agtaggttgt acagggcgct gtcctccagc
     1081 cagacgccag cgtttcagtt gccagaccca ggccagcact tccgctaccg cggcgtacag
     1141 ttgacccggg atttgttgac cgatctccgc atgtcgatac agcgctcgcg ccagcggcgg
     1201 cgcttcaagc gtagggacgt tattttcagc accaatttca cgaatgcgca gcgcgaccag
     1261 tcctgcacct ttagcgacca ctttcggtgc gctcattttg ttttcgtcat actgcaacgc
     1321 taccgaatag tgggttgggt tattgacaat gacatccgct ttcggcacat cggccatcat
     1381 ccgacgccgc gcggcagctc gctgcatctg acgtatccgc cctttaacat gggggtcgcc
     1441 ttcgctttgt ttgaactcat cacgaatatc ctgccgcgac attcgcagct ttttcaggtg
     1501 gctgaagatt tggaaaaaga cgtcaaatcc caccatcgga ataacaccaa gcaccaccag
     1561 cagtgcgcat agccctacca gatccatcgc attacccatg gcggtaatcg gagactcggc
     1621 cattaagcgc atcatctgcg gccagtgatg ccagagaaaa aaccccgtca cgctgccaac
     1681 caggatggtt ttcaaaattg ccttaagcaa ctccgcgcca gtctgtgccg agaacatccg
     1741 tttaatgccc ggtagcgggt tgagtttgga aaacttcggc tgcaaggatt tgccgctaaa
     1801 taccagcccc cccagcataa ccggagaaat aatcgccacc agcaccacgc cgctaatcag
     1861 cggtagcagc gccagcatgg cttctctgat cagcagaata atctgcccga ggatcaaatt
     1921 cgggtcattg ataatactgt gatcaaaatg cagcccagcg gagagcatgc ccgacaatcg
     1981 acgggccagc gacacaccgc caaaccagat aacacacacg cccactaaca aaatcagcag
     2041 cgaggtcagt tcacgggaac gcgggatttg cccctcttcc cgcgcttttt ctagtcggtg
     2101 aggtgtgggg gcttctgttt tgtcgtcgct ctcgtcagac acgtcgccaa tcctggaaag
     2161 agtattaagc gtaaatgatg ccagatcgca aagcgttcaa tggtttgagt aaggggcaaa
     2221 acaggcggga tttagggcgt ttatgcaaaa aacaggttca gttgcagaac gttttgcgaa
     2281 ccattttcca gttctgatat ttagccattt agcctataga atcgcccacc gcctctctgt
     2341 tagtttgcta ataattaaat tattacaaag gcgatcaaat ggaatggcaa caattgctta
     2401 actgcaaccg tcggaaagat aatctaaaaa aaaccagcat agaattttta catccccttg
     2461 ctagcggaag acaagagatc gagcgggatt acgaccgatt actttttgct gcgccaactc
     2521 gtcggcttgc ggataaaaca caagttttcc cgttagatca aaatgacagt atacggacga
     2581 ggctgactca ttctcacgaa gtagcaaact ttgctcgcgg aataggaatg agactcgctt
     2641 tcgagatgaa ggaacacatc tttggcgaaa tacccacggg gattgtcgtt gaacgcgacg
     2701 ttcctgctct gttagcagcg atcggactgg cacacgatct cggtaatcca ccatttgggc
     2761 atcaaggtga agcagcgatg cgcgcatggt ttaataaaaa tcttccaggc cttcttgata
     2821 aagaagtaaa cagcgaaata tataacgact ttctgcaatt tgatggaaac tcacaaaccc
     2881 tacgcctggt taccaaatta caaattatta acgataactt tggtctgaat ctgacctatg
     2941 ccacgctggc tgcactgatc aaatatcccc gagcttcata ttcatcggac aaacattgga
     3001 aaaagcacgg ttttttctat tctgagcatg aggttgtcat ggatgtctgg cagcaaacgg
     3061 gccttggcgt accaggtaat gatgccggtt gccgccaccc atttacctgg ataatggaag
     3121 cttgcgacga tattgcctac tctgtgcttg acgcagaaga taccatcaag aaaggatttg
     3181 cctcatacca ggatttgcgt gattttttga tgtcacaatc cgagccgaac gatgtaattc
     3241 aacatgttat tgaagctgta gaggccaaaa atgacgcctg gaaagagcaa agaaatgcac
     3301 tttcaccggc ggaaattaat gaactcagta tgcaaatgtt cagagtttat gccaccagtg
     3361 cattgattaa tgccgtagta gaagcctttc gcgaacaatt acccacgctg atgcagcgtg
     3421 actgcccgtt caaagattta atctcgcaaa gcaaaggtca cgctctctgc actgccctga
     3481 aaatgttcga ccgaac
//
LOCUS       DAMZFT010000268         3458 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.268, whole genome shotgun sequence.
ACCESSION   DAMZFT010000268 DAMZFT010000000
VERSION     DAMZFT010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3458)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3458)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3458
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.268"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(<1..281)
                     /locus_tag="PN977_005153"
     CDS             complement(<1..281)
                     /locus_tag="PN977_005153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001260625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor Ant"
                     /protein_id="HDI6045062.1"
                     /translation="MRTSLVTREEMIEAIEQHTACISTRDIPGVIANYFMITKQLYRR
                     KDKNAVHRILLTDIREYLLEQGHLNYATVAAEARKEAHRMKATNVKSEK"
     gene            complement(278..499)
                     /locus_tag="PN977_005154"
     CDS             complement(278..499)
                     /locus_tag="PN977_005154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000245706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
                     /protein_id="HDI6045063.1"
                     /translation="MVNANPCARPEFIWRFYSCQKRHYHFVIAPTEDEARSQLPDAPC
                     IFSARFSTDLRNSLSYWCLPVNASAQEGL"
     gene            939..1250
                     /locus_tag="PN977_005155"
     CDS             939..1250
                     /locus_tag="PN977_005155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000766012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6045064.1"
                     /translation="MKLGNLLAAAIVLTITACAGKAEKDPQYVAAEKCADLVITKSKA
                     LAEKGISGKYVLTSKLRTTSTSDGPFTSTQIRGIPYATSTFLDNGNEGSAWRQCMGPL
                     L"
     gene            complement(1284..1925)
                     /locus_tag="PN977_005156"
     CDS             complement(1284..1925)
                     /locus_tag="PN977_005156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002431873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maturation control protein"
                     /protein_id="HDI6045065.1"
                     /translation="MDKKICVVSMSVGKPASMTAAWINNELIMAERTSYPERRRDMEL
                     QLLRELREKEEKGFIVLVEEENSFITGRVGQRVRLRDPFMNGRPVLIEAMQIYKELER
                     QKAIKLPRKESGKYILHQSIFDSEHDKKGDEFFNINWSEITTEHVLTLLCCFATEYNN
                     VASADYIRAMAGEVEARQEPSLLSPLINIIRGTQRLAEKSVPHGILTGKGNYF"
     gene            2519..2740
                     /locus_tag="PN977_005157"
     CDS             2519..2740
                     /locus_tag="PN977_005157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000245706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
                     /protein_id="HDI6045066.1"
                     /translation="MVNANPCARPEFIWRFYSCQKRHYHFVIAPTEDEARSQLPDAPC
                     IFSARFSTDSRNSLSYWCLPVNASAQEGL"
     gene            2737..>3458
                     /locus_tag="PN977_005158"
     CDS             2737..>3458
                     /locus_tag="PN977_005158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015974252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor Ant"
                     /protein_id="HDI6045067.1"
                     /translation="MRTSLVTREEMIEAIEQHTICISARDIPGVIANYFMITKQLYRR
                     KDKNAVHRILLTDIREYLLEQGRLDYATVTAEARKEAHRMKASKPDVVVTKPLIESTP
                     LPAMDVIPSTGDTIDSQTLLKMVNEARKLCGENEIRNNDFLNRIKDELDGEFYETFVK
                     SHGTRAGRSFEVITMTYKQALRVAARESKAVRRSLIDKLEELQQVSAASPAIPQTLPE
                     ALRLAAELAEQKQLLEQKAHQL"
BASE COUNT          909 a          820 c          820 g          909 t
ORIGIN      
        1 attttttctg atttaacgtt agttgctttc attctgtgtg cctccttgcg tgcttcggct
       61 gcgacggttg cgtaattcag atgaccctgt tcgagcaggt attcacggat atcggttagc
      121 agaatgcggt gaaccgcgtt tttgtccttt ctccggtaaa gttgtttggt gatcatgaag
      181 tagttggcaa taacgcccgg tatatccctg gtactgatac aggcggtatg ctgttcaatt
      241 gcctcgatca tctcttcacg ggtgactaat gacgttctca tagtccctcc tgagcagaag
      301 cgttaacagg gagacaccag taactgagag agttgcgcaa atcagtggaa aaacgggcag
      361 agaaaatgca gggagcatcc ggaagctgag agcgagcctc atcttctgtc ggtgcaataa
      421 cgaagtgata gtgacgtttc tggcaggagt aaaagcgcca gataaattca gggcgtgcgc
      481 aaggattggc attaaccata gttacggcct catgtacagg tttaacaacc tgctacccgc
      541 tgctaaacgg gtggcagggc gtgacggggt tagcagactg gcgtacatga aaccagcagg
      601 ccgaagcctc cccatcacgc cccaccataa tttgggcgta acgcggtttt acggacataa
      661 aaataccgca atatcggaaa tctgcggttg tccgcatgta cattcaggct gctaaaccca
      721 gtcgcagaat ttgctacgac ggcatgaata taagcctgaa cggtaaggag atcaataggg
      781 ctatgcgcaa tggtaggata tttgctctaa taaatcaaat ttattagagc aatgagggtt
      841 gtgtgttatt tttgctcata ttgagcgtca tggaggattg aaatttgttt aaaaccttgg
      901 caacctactg tttttaatgc acttaataag gtatgcagat gaaactaggt aatttgttag
      961 ccgcagctat cgttttaacg ataactgcat gtgccggaaa agctgaaaaa gacccgcaat
     1021 atgtagccgc agagaaatgt gcagatttag ttatcacaaa atcaaaagct ctggcagaaa
     1081 aaggcatcag tggaaaatac gtacttacat caaaactgcg tacaacttcg acgtctgatg
     1141 gtccattcac atcaacacaa atcagaggca ttccatatgc aacctctacg tttcttgata
     1201 acggtaatga ggggagtgcc tggagacaat gtatggggcc gctattgtaa gaatgagttg
     1261 ttccccaaaa ggatttgggg atgttaaaaa tagtttccct ttccagttaa gattccgtgt
     1321 ggaacgcttt tttccgcaag tctctgtgtg ccgcgaatta tgttaatcag agggcttagt
     1381 aacgatggtt cctggcgtgc ctcaacttct ccagccattg ccctgatgta gtcggcgctt
     1441 gcaacgttgt tatattccgt tgcgaagcag cataacaacg tcagaacgtg ctctgtcgtt
     1501 atttcgctcc agttgatgtt gaaaaattca tcgccttttt tatcgtgttc ggaatcgaag
     1561 atgctttggt gaaggatgta tttgccggat tccttgcgcg gtaacttgat cgctttctgg
     1621 cgttccaatt ccttgtaaat ctgcattgct tcaatcagta ccggtctgcc gttcatgaag
     1681 ggatcacgca accttacacg ctggccaact cgaccggtaa taaagctgtt ttcctcttcc
     1741 accagcacga taaaaccctt ttcctctttt tctcgcaatt cgcgcagcag ctggagttcc
     1801 atatcgcggc ggcgttcagg gtagctggtc cgctcagcca ttatcagctc gttgttgatc
     1861 catgcagcag tcattgacgc cggtttgccg acgctcatcg aaacaacgca tattttctta
     1921 tccatagctc cccctacaaa aaagaaaagc caccagcggc ggcttagcaa tacaactgaa
     1981 ggtagcgccc ggtactcaga ctgtgccgtc catggaatat ttgaaaaggg atccatccgt
     2041 accgggcgtg tgatgattct gactgaagtc acttgtcagt tgtcaatcat ttaagattaa
     2101 aaataatata tttattagtg catacaaata aaacaataag tgtgcacatt gcaacctcgg
     2161 agaggaaaca ttcaaccaga ttgtattgct ctaataaatt tgatttgttg gaacaaataa
     2221 actactattg gacgccagac tattgatctt cccgtcgttc aggcttatag ttcctgcgtc
     2281 gtagcaaatt ctgcgaccgg gtttgacagc ctgaatactt gtgcggacaa ccgcatcact
     2341 ccgttattgc ggtatttttg tgtccgtaaa accgcgttac gcccgaatta tggtggggcg
     2401 tgatggggag gcttcggcct gctggtttca caagtgccag tctgtcaacc ctgtcacgtc
     2461 ctgccacctg tttgacagcg ggtagcaggt tgttaattca acttgtgagg ccgtaactat
     2521 ggttaatgcc aatccttgcg cacgcccgga atttatctgg cgcttctatt cttgtcaaaa
     2581 acgtcactat cacttcgtca ttgcaccaac agaagatgag gcccgttctc agcttcctga
     2641 cgctccctgc attttctctg cacgcttttc tactgactcg cgcaactctc tcagttactg
     2701 gtgcctccct gttaacgctt ctgctcagga ggggctatga gaacgtcatt agttacccgt
     2761 gaagaaatga tcgaggctat tgaacagcac acaatctgta tcagcgccag ggacattcct
     2821 ggtgttatag ccaactactt catgatcacc aaacaacttt accggagaaa ggacaagaac
     2881 gcggttcacc gcatcctgct aaccgatatc cgtgaatacc tgctcgaaca gggtcgtctt
     2941 gattatgcaa ccgtaaccgc cgaagcacgc aaggaggcac acagaatgaa agcaagcaaa
     3001 ccagatgttg tcgttacaaa gcctttgata gaatccacac cactaccagc catggatgtt
     3061 atccccagta cgggcgacac aatcgacagc cagacactgt taaagatggt caatgaggcg
     3121 cgcaagctat gtggggaaaa tgagatccgc aataacgatt ttctcaatag aattaaggat
     3181 gaacttgatg gggaatttta cgaaactttc gtaaaatccc atggcacaag ggctgggcgc
     3241 tcttttgagg tcataaccat gacctacaaa caagccctgc gagttgccgc gcgcgagtca
     3301 aaagcggtcc gccgttcgtt gatcgacaag ctggaggagt tgcagcaggt tagcgctgct
     3361 tctccagcga tcccccagac actccccgaa gccctccgcc tggctgccga gttggctgaa
     3421 caaaagcagc ttctggaaca gaaagcccac cagctaaa
//
LOCUS       DAMZFT010000269         3379 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.269, whole genome shotgun sequence.
ACCESSION   DAMZFT010000269 DAMZFT010000000
VERSION     DAMZFT010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3379)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3379)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3379
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.269"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(<1..310)
                     /locus_tag="PN977_005159"
     CDS             complement(<1..310)
                     /locus_tag="PN977_005159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020883197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDI6045068.1"
                     /translation="MVSSTTPSSGEYLLEMSGINKSFPGVKALDNVNLKVRPHSIHAL
                     MGENGAGKSTLLKCLFGIYQKDSGTILFQGKEIDFHSAKEALENGISMVHQELNLVLQ
                     R"
     gene            complement(371..1369)
                     /gene="mglB"
                     /locus_tag="PN977_005160"
     CDS             complement(371..1369)
                     /gene="mglB"
                     /locus_tag="PN977_005160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/glucose ABC transporter
                     substrate-binding protein MglB"
                     /protein_id="HDI6045069.1"
                     /translation="MNKKVLTLSAVMASMLFGAAAHAADTRIGVTIYKYDDNFMSVVR
                     KAIEQDAKAAPDVQLLMNDSQNDQSKQNDQIDVLLAKGVKALAINLVDPAAAGTVIEK
                     ARGQNVPVVFFNKEPSRKALDSYDKAYYVGTDSKESGIIQGDLIAKHWAANQGWDLNK
                     DGQIQFVLLKGEPGHPDAEARTTYVIKELNDKGIKTEQLQLDTAMWDTAQAKDKMDAW
                     LSGPNANKIEVVIANNDAMAMGAVEALKAHNKSSIPVFGVDALPEALALVKSGALAGT
                     VLNDANNQAKATFDLAKNLADGKGAADGTNWKIDNKVVRVPYVGVDKDNLAEFSKK"
     gene            complement(1649..2689)
                     /gene="galS"
                     /locus_tag="PN977_005161"
     CDS             complement(1649..2689)
                     /gene="galS"
                     /locus_tag="PN977_005161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708050.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalS"
                     /protein_id="HDI6045070.1"
                     /translation="MITIRDVARQAGVSVATVSRVLNNSTLVSADTREAVMKAVSELD
                     YRPNANAQALATQVSDTIGVVVMDVSDAFFGALVKAVDLVAQQHQKYVLIGNSYHEAE
                     KERHAIEVLIRQRCNALIVHSKALSDDELAQFMDNIPGMVLINRVVPGYAHRCVCLDN
                     LSGARMATRMLLNNGHQRIGYLSSSHGIEDDAMRKAGWMSALKEQDIIPPESWIGTGT
                     PDMPGGEAAMVELLGRNLQLTAVFAYNDNMAAGALTALKDNGIAIPLHLSIIGFDDIP
                     IARYTDPQLTTVRYPIASMAKLATELALQGAAGNIDPRASHCFMPTLVRRHSVATRQN
                     AAAITNSTNQAM"
     gene            complement(2831..>3379)
                     /gene="yeiB"
                     /locus_tag="PN977_005162"
     CDS             complement(2831..>3379)
                     /gene="yeiB"
                     /locus_tag="PN977_005162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF418 domain-containing protein"
                     /protein_id="HDI6045071.1"
                     /translation="QLAGMMLIGAALMRSGWLKGQFSLRHYRRTGFVLVAIGVTINLP
                     AIALQWQLDWAYRWCAFLLQMPRELSAPFQAIGYASLFYGFWPQLSRFKLVLAIACVG
                     RMALTNYLLQTLICTTLFYHLGLFMLFDRLELLAFVIPVWLANILFSVIWLRYFRQGP
                     VEWLWRQLTLRAAGPARSKTSR"
BASE COUNT          841 a          893 c          781 g          864 t
ORIGIN      
        1 aacgttgtaa taccaggttt aactcctggt gtaccatcga aataccattt tccagcgctt
       61 cttttgcaga atggaaatcg atctctttac cctggaataa aatggtgccg gagtcttttt
      121 gataaatacc aaacaggcat tttaataatg ttgattttcc tgcgccgttt tcccccatta
      181 atgcatggat agaatgtggc cggactttta aattaacgtt atcaagtgcc ttaacaccag
      241 gaaaagactt gttgataccg ctcatttcca acaagtattc cccggaagac ggagtcgttg
      301 agctgaccat ataattttac cttgttggcc ctacaataag ggcgcagtaa acgactgcgc
      361 ccaatcagtc ttatttcttg ctgaattcag ccaggttgtc tttatctacg ccaacataag
      421 gtacgcggac cactttgttg tcgattttcc agttggtgcc atcagccgca cctttgccat
      481 cggccaggtt tttcgccaaa tcaaaggtcg ctttcgcctg gttgttagca tcgttcagta
      541 cggtgcccgc cagtgcaccg gatttcacca gcgccagcgc ttctggcagc gcatcgacgc
      601 caaacaccgg aatgctggac ttgttgtgtg ctttcagtgc ttctaccgcg cccattgcca
      661 tcgcatcgtt gttggcgata accacttcga ttttgttggc gttcgggcca gatagccagg
      721 cgtccatctt atctttcgcc tgagcggtat cccacatagc ggtatctaac tgtaactgtt
      781 cagttttgat gcctttgtcg ttcaattctt taatcacgta agtggtacgt gcttctgcat
      841 ccggatggcc cggctcacct ttcagcagta cgaactgaat ctgaccgtct ttgttcagat
      901 cccaaccctg attcgccgcc cagtgtttag caatcaaatc gccctgaata atgccggact
      961 ctttggagtc agtgccaacg tagtaggctt tgtcgtagct atccagcgcc ttacgagacg
     1021 gttctttgtt gaagaaaacc accggcacgt tttgcccacg cgctttctca atcaccgtac
     1081 ccgcagctgc cgggtcaacc aggttgattg ccagtgcctt cacccctttc gccagcaata
     1141 cgtcgatctg atcgttctgc ttggactggt cattctgaga atcattcatc agcagctgaa
     1201 catctggcgc ggctttcgca tcttgctcaa tagccttgcg caccacagac ataaagttat
     1261 cgtcgtactt atagattgtt acaccaatgc gagtatcagc agcgtgtgca gcggcaccga
     1321 ataacatgct ggccatcaca gcagacaggg ttaacacctt cttattcatg gtatctccgg
     1381 tttttcttat gcagggtagt gcttgagata aatgctcggc ggggcagtag agttaatgaa
     1441 gtgttactga acgccgaagc tcacttttta aaattcgttc ttccatgccc ggtaacgctc
     1501 cagaaaacgg ctttaattgt tgttatgacg ctattacctc ggtgaaagtg attattcact
     1561 gttacatacg ggttacaacg ttaaaacggt gcaatcatag ctatcacatt gttaagatac
     1621 tgtgaaatca ctcacagatt gaaagcggtt acatcgcctg atttgttgag ttagtgatcg
     1681 ccgccgcatt ctggcgcgtt gcgacagaat gacgacgcac taacgttggc ataaaacagt
     1741 ggctggcacg aggatcaata ttgcctgctg ccccctgcaa ggccagttcg gtggctaatt
     1801 tagccattga agcaatggga taacgcacgg tcgttaattg cgggtcggtg taacgggcga
     1861 tgggaatatc atcgaaaccg atgattgaga gatgtaacgg aatcgcaatg ccattatctt
     1921 ttaatgctgt cagtgcgcca gcagccatat tgtcgttata agcaaataca gcggtaagtt
     1981 gtagattgcg ccccagcagt tcaaccatcg ccgcctcacc gcccggcatg tccggcgtgc
     2041 cagtgccaat ccagctttcc ggcggaataa tatcctgctc tttcaacgca ctcatccagc
     2101 ctgctttacg catggcgtca tcttcaatgc cgtggctgga agaaagataa ccaatacgtt
     2161 gatgaccgtt attcagcaac atgcgcgtcg ccattcgggc accgctgaga ttatccaggc
     2221 aaacgcaacg atgggcgtac cccggcacaa cgcggttgat taacaccata ccgggaatgt
     2281 tatccataaa ttgtgccagt tcatcatcac tcaatgcttt tgagtgaaca atcaacgcat
     2341 tacaacgctg gcgaattaac acctcaatgg cgtgacgctc tttttccgct tcatgatagc
     2401 tattgccgat tagcacgtat ttctgatgct gctgggcgac caaatccacc gcttttacca
     2461 gcgcgccgaa aaacgcatca gaaacgtcca tcaccaccac gccaatggtg tcgctaacct
     2521 gagttgccag cgcctgggca ttggcatttg gtcgataatc cagctcactc acggctttca
     2581 ttactgcttc acgcgtgtcg gcactgacca gcgtgctgtt attgagcact cgggaaaccg
     2641 ttgccacaga gacgccagcc tgacgcgcta catcacgaat ggtgatcata ttcactacct
     2701 gttatgagat tgcagtaaat gactgcttgc tggcggctat tttgtcagca ctgaatacag
     2761 gacttcgtga atcgagtcac agcaatggaa acggttacag ccgttttgtt aacgaatgtg
     2821 atccagatcg ttatcttgat gttttagatc ttgccggccc agctgcacgc aaagttaact
     2881 gacgccagag ccattccacc ggcccctggc ggaagtaacg cagccagata acagagaaga
     2941 gaatattcgc cagccatacc ggaataacaa acgccagcaa ttccagacgg tcaaacagca
     3001 taaacaaacc gaggtggtaa aaaagcgtgg tacaaatcag cgtttgcaat agatagttgg
     3061 tcagtgccat ccgtccgaca caggcgattg ccagcaccag cttaaagcgg cttaattgcg
     3121 gccagaagcc ataaaacagc gacgcatagc caatcgcctg aaacggcgca ctcagttccc
     3181 gcggcatttg aagtaagaat gcacaccagc gatacgccca gtccagctgc cattgcaggg
     3241 cgatggcagg aaggttaatg gtcaccccaa tcgccaccag cacaaaacca gtacgacgat
     3301 agtgacgtaa gctgaactgc cctttcagcc agccgctgcg catcaatgcg gcaccaatga
     3361 gcatcatccc tgccagttg
//
LOCUS       DAMZFT010000270         3375 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.270, whole genome shotgun sequence.
ACCESSION   DAMZFT010000270 DAMZFT010000000
VERSION     DAMZFT010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3375)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3375)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3375
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.270"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(<1..309)
                     /gene="sufE"
                     /locus_tag="PN977_005163"
     CDS             complement(<1..309)
                     /gene="sufE"
                     /locus_tag="PN977_005163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfuration protein SufE"
                     /protein_id="HDI6045072.1"
                     /translation="MALLPDKEKLLRNFLRCANWEEKYLYIIELGQRLPELRDEDRSP
                     QNSIQGCQSQVWIVMRQNAQGIIELQGDSDAAIVKGLIAVVFILYDQMTPQDIVNFDV
                     R"
     gene            complement(322..1542)
                     /gene="sufS"
                     /locus_tag="PN977_005164"
     CDS             complement(322..1542)
                     /gene="sufS"
                     /locus_tag="PN977_005164"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase SufS"
                     /protein_id="HDI6045073.1"
                     /translation="MTFSVDKVRADFPVLSREVNGLPLAYLDSAASAQKPSQVIDAEA
                     EFYRHGYAAVHRGIHTLSAQATEKMENVRKRASLFINARSAEELVFVRGTTEGINLVA
                     NSWGNSNVRAGDNIIISQMEHHANIVPWQMLCARVGAELRVIPLNPDGTLQLEMLPNL
                     FDEKTRLLAITHVSNVLGTENPLAEMITLAHQHGAKVLVDGAQAVMHHPVDVQALDCD
                     FYVFSGHKLYGPTGIGILYVKEALLQEMPPWEGGGSMIATVSLSEGTTWTKAPWRFEA
                     GTPNTGGIIGLGAALEYVSALGLNNIAEYEQNLMHYALSQLESVPDLTLYGPQNRLGV
                     IAFNLGKHHAYDVGSFLDNYGIAVRTGHHCAMPLMAYYNVPAMCRASLAMYNTHEEVD
                     RLVTGLQRIHRLLG"
     gene            complement(1539..2810)
                     /gene="sufD"
                     /locus_tag="PN977_005165"
     CDS             complement(1539..2810)
                     /gene="sufD"
                     /locus_tag="PN977_005165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HDI6045074.1"
                     /translation="MAGLPNSSNALQQWHHLFEAEVAKRSPQAQQHLQQLLRTGLPTR
                     KHENWKYTPLEGLTNSRFVSIAGEISPQQRDALALTLDAVRLVFVDGRYVSALSDATE
                     GSGYEVSINDDRQGLPDAIQAEVFLHLTESLAQSVTHIAVKRGQRPAKPLLLMHITQG
                     VAGEEVNTAHYRHHLDLAEGAEATVIEHFVSLNDARHFTGARFTINVAANAHLQHIKL
                     AFENPVSHHFAHNDLLLADDATAFSHSFLLGGAVLRHNTSTQLNGENSTLRINSLAMP
                     VKNEVCDTRTWLEHNKGFCNSRQLHKTIVSDKGRAVFNGLINVAQHAIKTDGQMTNNN
                     LLMGKLAEVDTKPQLEIYADDVKCSHGATVGRIDDEQMFYLRSRGINQQDAQQMIIYA
                     FAAELTEALRDEGLKQQVLARIGQRLPGGAR"
     gene            complement(2785..>3375)
                     /gene="sufC"
                     /locus_tag="PN977_005166"
     CDS             complement(2785..>3375)
                     /gene="sufC"
                     /locus_tag="PN977_005166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HDI6045075.1"
                     /translation="YEVTGGTVEFKGKDLLALSPEDRAGEGIFMAFQYPVEIPGVSNQ
                     FFLQTALNAVRSYRGQETLDRFDFQDLMEEKIALLKMPEDLLTRSVNVGFSGGEKKRN
                     DILQMAVLEPELCILDESDSGLDIDALKVVADGVNSLRDGKRSFIIVTHYQRILDYIK
                     PDYVHVLYQGRIVKSGDFTLVKQLEEQGYGWLTEQQ"
BASE COUNT          769 a          972 c          822 g          812 t
ORIGIN      
        1 gcgcacatcg aaattgacaa tatcctgcgg cgtcatctga tcgtagagaa taaagacgac
       61 cgcaattagc cctttcacaa tcgccgcatc gctgtcaccc tgcaattcaa taattccctg
      121 cgcattctgg cgcatgacaa tccacacctg actctggcag ccctgaatgc tattttgtgg
      181 gcttcggtct tcgtcgcgta attctggcag acgctggccc agctcaataa tgtagagata
      241 tttctcttcc cagttggcgc agcgtaaaaa attacgcagc aatttttctt tatccggcaa
      301 taaagccata gtgcctccct gttatcccag caaacggtga atacgttgca ggccggtcac
      361 cagacgatcc acttcttcat gggtgttata catggccagc gacgcccgac acatagcagg
      421 aacgttgtaa taggccatca atggcattgc gcagtgatgt ccggtacgca cagcaatgcc
      481 gtagttatcg agaaaactgc caacatcata ggcgtggtgt ttaccgagat taaaagcaat
      541 aacgccaagc ctgttctgcg ggccatagag agtgagatcc ggtacagatt ccagctgtga
      601 tagcgcgtag tgcatcagat tctgttcata ctcggctatg ttattaagcc ccagcgccga
      661 cacatactcc agcgccgcgc caagaccaat gatgcccccg gtattgggtg taccggcttc
      721 aaaccgccat ggtgctttgg tccaggtagt gccttcactc aggctgacgg tggcgatcat
      781 agaaccgccc ccttcccacg gcggcatctc ctgcaacaag gcttctttga cataaagaat
      841 gccaatcccg gtggggccat acagtttatg cccggaaaac acgtaaaaat cgcaatccag
      901 cgcctgaaca tccaccggat gatgcatcac cgcctgagcg ccatccacca gcacttttgc
      961 gccatgctgg tgcgcaagcg tgatcatttc cgccagcgga ttttccgttc ccagcacgtt
     1021 ggagacatga gtaattgcca gcaggcgagt tttctcatcg aacagattag gtagcatctc
     1081 cagttgcagc gtaccgtcgg ggttgagcgg gatcacacgc agctctgcgc caacgcgtgc
     1141 gcaaagcatc tgccagggga caatgttagc gtggtgctcc atctgactga tgatgatgtt
     1201 atcgcccgcc cgcacattgc tgttacccca gctattggcg accagattga tcccttccgt
     1261 cgtgccgcgg acgaacacca gctcttccgc cgaacgggca ttaataaaca gcgatgcccg
     1321 cttgcgcacg ttctccattt tctcggtcgc ctgggcgctt aaggtatgaa taccgcgatg
     1381 caccgccgcg tagccatgac gataaaactc ggcctcggcg tcaatcacct ggctcggttt
     1441 ctgtgcactg gcggcgctgt cgagataagc cagcggcaaa ccgttcacct cacgagaaag
     1501 caccggaaag tcggcccgca ctttgtcgac ggaaaaagtc atcttgcacc tcctggcagc
     1561 cgttgaccga ttcgggccag cacctgctgt ttaagaccct catcacgcag tgcttccgtc
     1621 agttcagcag caaaggcgta aatgatcatc tgctgggcat cctgctgatt gatcccgcgc
     1681 gagcgcagat agaacatctg ttcatcatca atacgcccca ccgtcgcgcc gtggctgcat
     1741 ttcacatcat ctgcatagat ttccagctgc ggtttcgtat ccacttccgc cagtttgccc
     1801 atcagcagat tattgttggt catctgacca tccgttttga tggcgtgctg cgcgacgttg
     1861 atcaaaccgt taaataccgc gcggcctttg tcgctgacga tagttttatg caactgtcga
     1921 ctgttacaaa aacctttatt gtgttccagc caggtacggg tatcacacac ctcgtttttc
     1981 accggcatcg ccaggctatt gatccgcagc gtgctgtttt cgccattgag ttgcgtactg
     2041 gtgttgtgtc gtaacactgc gccacccagc aggaaactgt ggctaaatgc ggtggcatcg
     2101 tcagccagca acaaatcgtt atgggcaaag tggtgactga ccgggttttc aaacgccagc
     2161 ttgatatgct gcaagtgggc gttcgctgcg acgttgatag tgaaccgtgc gccggtgaag
     2221 tgacgagcat cattaaggct gacaaaatgt tcgatcaccg ttgcttcggc accttccgcc
     2281 agatccagat gatgtcggta atgggcagta ttcacctctt cacctgccac gccctgggtg
     2341 atatgcatta acagcaatgg ctttgccggt cgttgaccgc gcttcacggc gatatgcgtc
     2401 acgctttgcg ccaggctttc cgtcaaatgc agaaacactt ccgcctgaat agcgtcgggt
     2461 aacccctgac ggtcgtcgtt aatgctcact tcatacccgc tgccttcagt cgcatcgctc
     2521 agtgctgaca cgtaacgtcc atcgacaaac accagccgca cggcgtctaa cgttaacgct
     2581 aaggcatctc gctgctgtgg ggatatctct cccgcaatgc tgacaaaccg gctattggtc
     2641 agcccttcca gcggcgtata tttccagttt tcatgtttac gtgtcggcag tccggtacgc
     2701 agcaattgtt gtaaatgctg ctgtgcttgc ggggagcgtt tcgccacttc agcttcaaac
     2761 aaatgatgcc actgttgcag tgcgttactg ctgttcggta agccagccat aaccctgctc
     2821 ctccagttgt ttgaccaacg tgaaatcgcc ggatttcaca attcgtccct gatacagcac
     2881 atggacgtaa tcaggcttga tgtagtcgag aatgcgttgg tagtgcgtaa caatgatgaa
     2941 tgaacgcttg ccgtcacgca gcgagttcac gccatcggca accactttta atgcgtcaat
     3001 atccagccca gagtccgact catcaagaat gcataactcc ggttccagca ccgccatttg
     3061 caaaatatcg ttgcgctttt tctcgccgcc ggaaaaacca acgtttaccg aacgggttaa
     3121 taaatcctcc ggcatcttca ggagagcgat tttctcttcc atcaaatcct gaaaatcaaa
     3181 gcggtcgagc gtttcctggc cgcgatagct gcgcaccgca ttaagtgccg tttgcaggaa
     3241 aaactggtta ctgacgccgg gaatttccac cggatactgg aaggccataa agatgccttc
     3301 acctgcacga tcttccggcg acagcgcaag caaatctttg cctttgaact caaccgtgcc
     3361 gcccgtcacc tcata
//
LOCUS       DAMZFT010000271         3360 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.271, whole genome shotgun sequence.
ACCESSION   DAMZFT010000271 DAMZFT010000000
VERSION     DAMZFT010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3360)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3360)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3360
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.271"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(24..326)
                     /locus_tag="PN977_005167"
     CDS             complement(24..326)
                     /locus_tag="PN977_005167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001245884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6045076.1"
                     /translation="MRNRANTSPRDEAAFINGGTAGLDNTKKSSDKSSVVSKAKPVSI
                     SFFEENLKDIDALITAEMVSGKGRVNRSDVVRAAVEALKTLSKTEVSRLIAEVKQR"
     gene            complement(323..949)
                     /locus_tag="PN977_005168"
     CDS             complement(323..949)
                     /locus_tag="PN977_005168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010260829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family plasmid-partitioning AAA ATPase"
                     /protein_id="HDI6045077.1"
                     /translation="MIIVLGSQKGGVGKSTLAVSIAAYLMSLGNRVIIVDADDQKSVL
                     TWYNNRPEDLPHIPVTGATGNIKAMLKEHEKSYDFVIADCAGRDSAEMRSGLMAADVF
                     ISPLRPSQMDLDVVPHTCSVFTAAKDFNEDVRGYLVLNMTPTNMFVNEANEAAEVLKD
                     YPEMHLANSRVCDRKAHRDAWAESMTIFETQNDKAQQEIEALVKEVIL"
     gene            complement(1129..2400)
                     /locus_tag="PN977_005169"
     CDS             complement(1129..2400)
                     /locus_tag="PN977_005169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001744069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HDI6045078.1"
                     /translation="MFALADINSFYASCEKVFRPDLRNEPVIVLSNNDGCVIARSPEA
                     KALGIRMGQPWFQVRQMRLEKKIHVFSSNYALYHSMSQRVMAVLESLSPAVEPYSIDE
                     MFIDLRGINHCISPEVFGHQLREQVKSWTGLTMGVGIAPTKTLAKSAQWATKQWPQFS
                     GVVALTAENRNRILKLLGLQPVGEVWGVGRRLTEKLNALGINTALQLAQANTAFIRKN
                     FSVILERTVRELNGESCISLEEAPPAKQQIVCSRSFGERITDKDAMHQAVVQYAERAA
                     EKLRGERQYCRQVTTFVRTSPFAVKEPCYSNAAVEKLSLPTQDSRDIIAAACRALNHV
                     WREGYRYMKAGVMLADFTPSGIAQPGLFDEIQPRKNSEKLMKTLDELNQSGKGKVWFA
                     GRGTAPEWQMKREMLSPAYTTRWTDLPVAQL"
     gene            complement(2400..2837)
                     /gene="umuD"
                     /locus_tag="PN977_005170"
     CDS             complement(2400..2837)
                     /gene="umuD"
                     /locus_tag="PN977_005170"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="HDI6045079.1"
                     /translation="MSTVYHRPAAPSGDDSYVRPLFADRCQAGFPSPATDYAEQELDL
                     NSYCISRPAATFFLRASGESMNQAGVQNGDLLVVDRAEKPQHGDIVIAEIDGEFTVKR
                     LLLRPRPALEPVSDSPEFRTLYPENICIFGVVTHVIHRTRELR"
     gene            complement(2834..3082)
                     /locus_tag="PN977_005171"
     CDS             complement(2834..3082)
                     /locus_tag="PN977_005171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001697964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HDI6045080.1"
                     /translation="MIRIEILFDRQSTKNLKSGTLQALQNEIEQRLKPHYPEIWLRID
                     QGSAPSVSVTGARNDKDKERILSLLEEIWQDDSWLPAA"
BASE COUNT          789 a          855 c          756 g          960 t
ORIGIN      
        1 tgttatcttg ttatctcaag gtgttatctt tgttttactt ctgctatcaa tcgtgatacc
       61 tctgtttttg atagtgtttt aagtgcttct accgccgccc tgacaacatc agaacgattc
      121 actctgcctt tacctgaaac catctcagcc gttataaggg catcaatgtc ttttaggttt
      181 tcttcaaaaa aagaaatact cactggtttt gctttgctta caaccgatga tttatctgat
      241 gactttttgg tattatcaag gccagccgtg ccaccgttaa taaatgctgc ctcgtcacga
      301 ggtgaagtgt ttgctctatt tctcacagaa taacctcttt cactaacgct tcgatttctt
      361 gctgggcttt atcattttgc gtttcaaaaa ttgtcattga ttcggcccaa gcatctcgat
      421 gtgctttacg atcacatacc cgggagtttg ccagatgcat ttcagggtaa tccttaagta
      481 cttcagctgc ctcattggct tcattaacaa acatatttgt gggcgtcatg tttaaaacga
      541 gatatccccg aacatcctca ttaaaatctt ttgctgctgt aaaaacggag catgtatgag
      601 gcacaacatc caggtccatc tgagatgggc gcaaaggaga aatgaataca tctgcggcca
      661 tcaatccact acgcatttct gcgctatcac ggccagcaca gtctgcaata acaaaatcat
      721 aggatttttc gtgttccttt aacatggctt tgatgttacc tgtcgcgcct gtaaccggaa
      781 tatgagggag atcttcaggg cgattgttgt accaggttaa tactgatttc tggtcatctg
      841 cgtcgacaat aatcactcta ttgcctagtg acatcagata cgcagctata gataccgcca
      901 gtgtactttt acctacccca cccttttgac tacctaatac tattatcatt atagcctcat
      961 attaagttgt taagttaaca acttaatatt gccatctgaa cgcgaatcat gcaattattt
     1021 ttgttatatt aacaacttaa caagttgtta agtatttgat ttatagatta attaagataa
     1081 caagataaca agataacaag ataacaatat tatgggtgga ttacatattc ataactgggc
     1141 tactggcaaa tctgtccatc tggtggtata cgcaggagaa agcatttccc gtttcatttg
     1201 ccactcaggg gcggttcctc gccccgcaaa ccacactttc ccttttcccg actggttcag
     1261 ttcatcgagt gttttcatta acttttcact gtttttacgg ggctggattt catcaaataa
     1321 tcccggctgc gctatacccg atggtgtgaa atcagccagc atgactcctg ccttcatata
     1381 gcggtaccct tcacgccaga catggtttaa ggccctgcat gcggcggcaa taatgtcccg
     1441 gctgtcctgt gtgggcaatg aaagcttttc cacagcggca ttgctgtaac agggttcttt
     1501 tactgcaaag ggggatgtcc gtacaaatgt cgtcacctgc cggcaatact gacgttcccc
     1561 acgtagtttc tctgcggccc gctctgcata ctgaacaaca gcctggtgca tggcatcttt
     1621 gtctgtgatt cgttcaccaa aactgcgact acagacaatc tgctgttttg ccggtggtgc
     1681 ttcttccagg gatatgcagg actcgccgtt gagttcgcgt accgtacgct caagaatgac
     1741 gctgaagttt ttccggatga atgccgtgtt agcctgcgcc agctgcagtg ctgtgttaat
     1801 acccagcgca ttcagctttt ccgtcagtct gcgtcctact ccccagacct caccaactgg
     1861 ctgcagcccc agtagcttca agatccgatt acggttttct gccgtcagcg cgaccacacc
     1921 ggaaaactgt ggccattgct ttgttgccca ctgtgcactt ttagccagcg tttttgtagg
     1981 cgcaatgccc acccccatgg tgagtcctgt ccagctcttt acctgttccc tgagctgatg
     2041 accaaaaacc tccggagaga tgcaatgatt tatcccccgc aaatcaatga acatttcatc
     2101 aattgagtag ggctcaactg cgggagaaag cgactccaga acagccataa cccgttggct
     2161 catgctgtgg tacagcgcat aattgctgga aaatacatgt attttcttct ccaggcgcat
     2221 ctgtctcacc tgaaaccagg gctgccccat tctgatgcca agggcttttg cctccgggct
     2281 gcgcgcgatc acacagccat cgttattgct gagtacgatg accggttcgt tgcgaaggtc
     2341 cgggcggaaa actttttcac atgaggcgta gaaactgttg atatcagcca gtgcaaacat
     2401 cagcgtaact cccgcgtcct gtgtatcacg tgagtgacaa caccaaaaat acagatgttt
     2461 tccggataca gtgtgcggaa ctccgggctg tctgaaaccg gctccagtgc cgggcgtggg
     2521 cgcaacagca gtcgtttgac ggtgaactca ccgtcgatct cagcgataac gatgtccccg
     2581 tgttgtggtt tttcggccct gtccactacc agcagatcac cattctgcac gccagcctgg
     2641 ttcatcgatt caccgctggc gcgcagaaag aaggtggctg caggtctgct gatgcaatag
     2701 ctgttcagat ccagttcctg ctcagcataa tcagtggcag gcgaaggaaa accggcctgg
     2761 caacgatcgg caaacaacgg tcgcacgtaa ctgtcatcgc ctgaaggggc agccggacgg
     2821 tgataaacgg tactcatgct gcaggcagcc agctgtcgtc ctgccagatt tcttccagca
     2881 gagacaggat acgttcttta tccttgtcgt tgcgggctcc tgtgacagat acagacggtg
     2941 cgctgccctg gtctatacgc agccagattt ccgggtagtg tggtttcaga cgttgttcga
     3001 tttcattctg tagcgcctga agtgtgcctg atttgaggtt ttttgtgctc tggcggtcga
     3061 aaagaatttc aatgcggatc atgttctcat ctcccttctc tattgctgta tgtatataca
     3121 gtattctaat aactgaatcc gcacacagtc aaatgtcatt gttctgacta aaaaacgctc
     3181 aattctctga gggtgtggct gtatgcctgc tgtggactgc tgtgtccagc ttatccacag
     3241 cattttgtgc acggttctgt ggacaaaata cctggttacc caggccgtgc cggcacgtta
     3301 accgggctgc atccgatgca agtgtgtcgc tgtcgccggc atcctcacca ggtcactcag
//
LOCUS       DAMZFT010000272         3230 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.272, whole genome shotgun sequence.
ACCESSION   DAMZFT010000272 DAMZFT010000000
VERSION     DAMZFT010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3230)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3230)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3230
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.272"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(<1..464)
                     /locus_tag="PN977_005172"
     CDS             complement(<1..464)
                     /locus_tag="PN977_005172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDI6045081.1"
                     /translation="MSKPKYPFEKRLEVVNHYFTTDDGYRIISARFGVPRTQVRTWVA
                     LYEKHGEKGLIPKPKGVSADPELRIKVVKAVIEQHMSLNQAAAHFMLAGSGSVARWLK
                     VYEERGEAGLRALKIGTKRNIAISVDPEKAASALELSKDRRIEDLERQVRFL"
     gene            544..696
                     /gene="hokA"
                     /locus_tag="PN977_005173"
     CDS             544..696
                     /gene="hokA"
                     /locus_tag="PN977_005173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001135724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokA"
                     /protein_id="HDI6045082.1"
                     /translation="MPQKYRLLSLIVICFTLLFFTWMIRDSLCELHIKQGSYELAAFL
                     ACKLKE"
     gene            complement(885..1097)
                     /gene="cspA"
                     /locus_tag="PN977_005174"
     CDS             complement(885..1097)
                     /gene="cspA"
                     /locus_tag="PN977_005174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone/antiterminator CspA"
                     /protein_id="HDI6045083.1"
                     /translation="MSGKMTGIVKWFNADKGFGFITPDDGSKDVFVHFSAIQNDGYKS
                     LDEGQKVSFTIESGAKGPAAGNVTSL"
     gene            complement(1378..1668)
                     /locus_tag="PN977_005175"
     CDS             complement(1378..1668)
                     /locus_tag="PN977_005175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator"
                     /protein_id="HDI6045084.1"
                     /translation="MEYKDPMHELLSSLEQIVFKDETQKITLTHRTTSCTEIEQLRKG
                     TGLKIDDFARVLGVSVAMVKEWESRRVKPSSAELKLMRLIQANPALSKQLME"
     gene            2102..2812
                     /locus_tag="PN977_005176"
     CDS             2102..2812
                     /locus_tag="PN977_005176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3053 domain-containing protein"
                     /protein_id="HDI6045085.1"
                     /translation="MATGKSCSRWFAPLAALLMVVSLSGCFDKEGDQRKAFIDFLQNT
                     VMRSGERLPTLTADQKKQFGPFVSDYAILYGYSQQVNQAMDSGLRPVVDSVNAIRVPQ
                     DYVTQSGPLREMNGSLGVLAQQLQNAKLQADAAHSALKQSDDLKPVFDQAFTKVVTTP
                     ADALQPLIPAAQTFTQQLVMVGDYIAQQGTQVSFVANGIQFPTSQQASEYNKLIAPLP
                     AQHQAFNQAWTTAVTATQ"
     gene            complement(2862..>3230)
                     /locus_tag="PN977_005177"
     CDS             complement(2862..>3230)
                     /locus_tag="PN977_005177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrB"
                     /protein_id="HDI6045086.1"
                     /translation="FVCLILPLTDETHHLFGAEQFAKMKSSAIFINAGRGPVVDENAL
                     IAALQKGEIHAAGLDVFEQEPLSVDSPLLSMANVVAVPHIGSATHETRYGMAACAVDN
                     LIDALQGKVEKNCVNPHVAD"
BASE COUNT          855 a          746 c          737 g          892 t
ORIGIN      
        1 tcaagaaatc gaacttgcct ttcaagatcc tcaatacgtc ggtcttttga cagctccaat
       61 gctgatgccg ctttttctgg atcaactgat attgcaatgt ttcttttggt gccaatcttg
      121 agcgcgcgta aaccagcttc tccgcgctct tcataaacct tcagccacct ggctacagaa
      181 ccactaccag caagcataaa gtgagcagca gcctgattaa gggacatgtg ctgctcgatc
      241 acagctttca cgaccttaat acgcaactct ggatcagcac taacaccttt aggtttggga
      301 attaaacctt tttctccatg tttttcatag agggcaaccc atgtcctgac ctgggttcgg
      361 gggacaccaa aacgtgccga gatgatcctg taaccatcat cagtagtgaa gtagtgattc
      421 acgacttcaa ggcgcttttc aaaagggtat tttggctttg acatatcagg ggctattcca
      481 tttcatcgtc caacaaaatg ggtgcagtac atactcgttg gaaatcaaca caggaggctg
      541 ggaatgccgc agaaatatag attactttct ttaatagtga tttgtttcac gcttttattt
      601 ttcacctgga tgataagaga ttcactgtgt gaattgcata ttaaacaggg gagttatgag
      661 ctggcggcgt ttttagcctg caaattgaaa gagtaagcgt cttcggcggg aagttattcc
      721 cgcctttctt acggcgttgc gcattctcat tgcacccaaa tttattcttc acaaaaataa
      781 taatagattt tattacgcga acgattattt atttcctgaa taaacaaata aaaaaatccc
      841 cgccatttgg cagggatctt agattctgtg cttttaagca gagattacag gctggttacg
      901 ttaccagctg ccgggccttt agcgccgctt tcgatggtga aggacacttt ctgaccttcg
      961 tccagagatt tgtaaccatc gttctggata gcagagaagt gtacgaacac gtctttagag
     1021 ccatcgtcag gagtgatgaa gccgaagcct ttgtcagcgt tgaaccattt tacgatacca
     1081 gtcattttac cggacatagt gtattacctt taataattaa gtgtgccttt cggcgatatg
     1141 gcgtgcttta cagattttga agcgttaaag gaatgtgcac tacgaggggt atcaacgata
     1201 actcttgaag ggacttgcct tactacactg ctttaatggt ctgtacgtca aaccgttgat
     1261 gtgcattaag ccacgcattg gcgggtgatg caacaattat ttttcatatt tatgattaat
     1321 cggttggaaa acggtcctgt catcaggacc gtaaacagca ataaagtgga taaaagtcta
     1381 ttccatcaac tgcttactta atgccgggtt ggcttgaatc aaacgcatca attttagttc
     1441 ggcacttgaa ggcttcacgc gtctggattc ccattccttt accatggcga ctgatacgcc
     1501 caaaacccgg gcgaaatcat cgatttttaa ccctgtccct tttcgtaact gctcaatttc
     1561 ggtacaggac gttgttctgt gcgtcagggt aattttctgc gtttcatctt taaaaacaat
     1621 ctgttccagg ctgctcaaca gctcatgcat tggatcttta tattccattg aaaactcctc
     1681 aaatcacact gcgggatcgt gaattgcatc gaagctcatt aagaatagtc gggaaaaaca
     1741 gacacagcgg ggtgtagtca gggcatgctc tggctatgat tcgattcagc cagtcattta
     1801 gcgtgactta tagccaccta acgatctgat aaatgccgat atatctctta ccactcccgg
     1861 tttgtaaaga tttcgaaaat ctaacgttct gagaaggcgt aaatctctgg ctacaaaaaa
     1921 agcaacggca ctatcatttt ttaacaatga aacaacaaat taaccttgtt tcattttctc
     1981 catgatttta ccaggaagat tacgtagaaa gggtatcctg caagcctgtc ctggactatc
     2041 cttgtcacgt cagacacgcg tgtgtcgttg tgcgcttttt ttgggtgaaa ggagtaagaa
     2101 aatggcgaca ggaaagtcct gctctcgctg gtttgcgcct cttgcggcgt tattaatggt
     2161 agttagcctg agtgggtgtt ttgataaaga aggcgatcag cgtaaagcgt ttatcgactt
     2221 cctgcagaat acagtgatgc gtagcggtga acgtctacca accctgactg ccgatcagaa
     2281 aaaacagttt ggtccttttg tctctgatta cgcgattctg tatggttatt ctcagcaggt
     2341 gaatcaggcg atggattccg gtctgcgccc ggttgtagac agcgttaacg caattcgcgt
     2401 accgcaggat tacgttacgc aaagcggccc actgcgtgaa atgaacggtt ctctgggtgt
     2461 gctggcgcaa cagctgcaaa atgcgaagtt gcaggccgat gctgcacact ctgcgctaaa
     2521 acagagcgat gacctgaaac cggttttcga tcaggcattc accaaagtgg taacgacgcc
     2581 agctgatgca ttgcaaccgt tgattccggc ggcacaaact ttcacgcaac aactggtgat
     2641 ggttggagac tacattgctc agcaaggtac tcaggtgagc tttgtcgcaa atggcatcca
     2701 gttcccgacg tcacagcagg caagtgaata taacaaactg attgcgccat taccggcaca
     2761 gcatcaggcg tttaatcagg cctggactac agcagttact gcaacccaat aaagagtaaa
     2821 agcccgagcg attatcgggc tttactccac gcagtcgcgg cttagtccgc gacgtgcgga
     2881 ttcacacagt tcttctcaac ctttccttgt aacgcatcaa tcaaattatc cacggcacag
     2941 gcggccatgc cataacgcgt ctcatgggtg gcagatccaa tatgcggtac tgcgacgacg
     3001 ttggccattg agagcaacgg cgaatctaca gacagtggct cttgttcgaa gacatccagc
     3061 ccggcggcgt gaatttcccc tttctgcaat gcagcaatca gtgcattttc atcaaccacc
     3121 gggccacgtc cggcattaat gaaaatagcg gaggatttca ttttggcgaa ttgttctgcg
     3181 ccaaacagat gatgcgtttc atcagttaac ggcaggatca ggcaaacgaa
//
LOCUS       DAMZFT010000273         3195 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.273, whole genome shotgun sequence.
ACCESSION   DAMZFT010000273 DAMZFT010000000
VERSION     DAMZFT010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3195)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3195)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3195
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.273"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..220
                     /locus_tag="PN977_005178"
     CDS             <1..220
                     /locus_tag="PN977_005178"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDI6045087.1"
                     /translation="EGIALGMAQGMERGKLEGRMEGRMEGRAEGRLEGQLEGKLETAR
                     NMLAEGMGFQTIMKITGLSEEQLKKISH"
     gene            complement(347..1849)
                     /gene="istA"
                     /locus_tag="PN977_005179"
     CDS             complement(347..1849)
                     /gene="istA"
                     /locus_tag="PN977_005179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HDI6045088.1"
                     /translation="METCLKILQLKFEQHQTNRTIALTLNISPSTVSDVLSRFKNTSI
                     SWPLPESMTTQRLEVLIFPGKQAPEMTPALPDWLYIDTELRRVGVTRQLLWMEYKHQA
                     GEYALGYSQFCRRYLHWKKSVRYSMRQEHRAGEKLFIDFCGPTVPVVNPETGEERRVA
                     IFVAVMGASNYTYVEACEGQDLMSWLNANSRCLTFLGGVPKLLIPDNLKSAVKKVDRY
                     EPVINESYQAFAEHYNTVILPARPRKPKDKAKAETAVLIVERWLLARIRNETFFTLRS
                     LNSRLRELLVELNNRPMKGYGNQTRAERFALLDAPALSALPVSEYEFAEYKTVKVAPD
                     YHVEYAHHWYSVPHELIGQRLSLKATQTVIQLWHQGQCVAQHPRSLHEYKHTTNPLHM
                     PAAHQAHSRWTPESLQEAARKIDPYTAMTVDSMLNSKRHPEQAYRTVLGLLSLKKHYG
                     RERLEKACHVARECRTSERRFIENLLRNNRENLSLRKKRGRRRETYQKSS"
     gene            1967..2392
                     /locus_tag="PN977_005180"
     CDS             1967..2392
                     /locus_tag="PN977_005180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001373091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence hypothetical
                     protein"
                     /protein_id="HDI6045089.1"
                     /translation="MELQDWRKEPRKNYSNEFKLRMVELASQPGACVAQIARENGVND
                     NVIFKWLRLWQNEGRVSRRLPVTTSSDTGVELLPVEITPDEQKEPVAAIAPSLSTSTQ
                     TRVSASSCKVEFRHGNMTLENPSPELLTVLIRELTGRGR"
     gene            2389..2739
                     /gene="tnpB"
                     /locus_tag="PN977_005181"
     CDS             2389..2739
                     /gene="tnpB"
                     /locus_tag="PN977_005181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017901265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HDI6045090.1"
                     /translation="MISLPSGTRIWLVAGVTDMRKSFNGLGEQVQHVLNDNPFSGHLF
                     IFRGRRGDTVKILWADADGLCLFTKRLEEGQFIWPAVRDGKVSITRSQLAMLLDKLDW
                     RQPKTSSRNSLTML"
     gene            2770..>3195
                     /locus_tag="PN977_005182"
     CDS             2770..>3195
                     /locus_tag="PN977_005182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDI6045091.1"
                     /translation="MSQKYLIRIAELERLLSEQAEALRQKDQQLSLVEETEAFLRSAL
                     TRAEEKIEEDEREIEHLRAQIEKLRRMLFGTRSEKLRREVELAEALLKQREQDSDRYS
                     GREDDPQVLRQLRQSRHRRPLPAHLPREIHRLEPEESCCP"
BASE COUNT          792 a          759 c          851 g          793 t
ORIGIN      
        1 ggaaggcatt gcgctgggca tggcgcaggg tatggagcgt ggtaagctgg aaggcagaat
       61 ggaaggcaga atggaaggcc gtgccgaagg gcgactcgaa ggacaactgg aaggcaaact
      121 ggaaaccgcg cgtaatatgc tggctgaagg gatggggttc cagacaatca tgaaaattac
      181 ggggctgagt gaagaacagc tgaaaaaaat cagccactga agcgcagggc gtgatgtgta
      241 tcacgccctg ttttttatac aaaggtgtga actgaagatg acggagaggc cgtgaaaaag
      301 aaaatcatga cgggtgaaaa cgtgatgcag gcagtcatca cgcgtattaa ctggattttt
      361 gatacgtttc cctccgtctg cctctctttt tccggaggga aaggttttcc ctgttattac
      421 gcagaaggtt ttcgatgaac cttctttccg acgttctgca ctcccgggca acatgacagg
      481 ctttttccag acgctcacga ccatagtgtt ttttcaggct cagcagcccc aggactgtcc
      541 ggtaagcctg ttcaggatga cgtttactgt tcagcatgct atccactgtc attgctgtat
      601 aaggatctat ctttctggct gcttcctgaa gactttccgg tgtccatctg ctgtgtgcct
      661 gatgtgctgc aggcatgtgt agagggttgg tcgtgtgctt gtactcatgg aggctgcgcg
      721 gatgttgtgc cacgcattgc ccctgatgcc agagttggat cactgtctga gtggctttca
      781 gggataaccg ttgtccgatc agttcatgag gtactgagta ccagtggtgg gcgtattcaa
      841 catggtaatc aggtgcaact ttcaccgttt tgtattcagc gaactcatac tctgatactg
      901 gcagggctga taatgcaggg gcatccagca gggcaaaacg ctctgcacgc gtctggttgc
      961 cgtatccttt catgggacgg ttattcagct ctaccaacaa ctcgcgtaga cgactgttca
     1021 ggctcctgag tgtaaagaag gtctcgttgc gaatgcgggc aagcagccat cgttcgacga
     1081 taagtacagc cgtttcggct ttcgctttat cctttggttt tcttggtctt gctgggagta
     1141 tcactgtatt gtagtgctct gcgaatgctt ggtaactctc attgatgacc ggttcgtagc
     1201 gatcgacttt tttgacagca cttttaaggt tatccgggat cagcagtttt ggtacaccac
     1261 caaggaaggt cagacatcgg ctgttggcgt ttagccacga catcagatcc tgaccttcgc
     1321 acgcttcaac ataggtatag ttagatgctc ccatcacggc tacaaagatg gccactctgc
     1381 gttcttctcc ggtttccgga ttaacaacag ggactgtagg accacagaag tcgataaaga
     1441 gtttttcacc agcacgatgt tcctgccgca tggagtaacg cacgcttttt ttccagtgca
     1501 gataacgacg acagaactgt gagtatccca gagcatattc accagcctga tgtttgtatt
     1561 ccatccataa gagctgacga gtgactccca cacgacgtag ttcggtatca atgtagagcc
     1621 agtcaggcag tgccggcgtc atttccgggg cttgcttgcc aggaaagata agcacctcaa
     1681 gacgttgagt tgtcatcgat tcaggcaaag gccacgatat tgacgtgttc ttaaagcggg
     1741 acaatacatc ggaaacagtc gagggactga tgttcagtgt aagtgcgata gtccggtttg
     1801 tctgatgctg ctcgaactta agttggagaa tttttaaaca ggtttccata gaactccggg
     1861 agattttctt tttagccatc agtaagcgta aactgaccgc cgtatgtagc catcagacga
     1921 gaattggtaa cttagacgcc catctgatat agacggacat ctaagtatgg aattacagga
     1981 ctggcgaaaa gaacctcgta aaaactattc gaatgaattc aaacttcgta tggtggaact
     2041 ggcatcacaa cctggagctt gtgttgcaca gattgcacgt gaaaatggcg tcaatgataa
     2101 tgttattttc aaatggctca ggctctggca gaacgaaggg cgtgtttcgc ggcgtcttcc
     2161 ggtaacgacc tcttctgaca ctggcgttga attattacct gtagaaataa cgccggatga
     2221 gcagaaagaa cctgtggcgg ccattgcgcc gtctttatcc acttccactc agaccagagt
     2281 cagtgccagt tcctgcaagg tggaattccg tcacggtaac atgacgctgg aaaatccatc
     2341 gccagagctg ctcacagtgt tgatccgtga actgaccggg aggggaagat gatctcactc
     2401 ccatcaggta cccgtatctg gctcgttgcc ggcgttaccg atatgcgtaa atccttcaac
     2461 ggactgggag aacaggtaca acatgtgctg aatgataatc ccttctccgg tcacctgttt
     2521 atcttccgtg gccgacgggg tgacaccgtc aaaattcttt gggctgatgc tgatggtctg
     2581 tgcctgttca ccaaacgcct ggaggaaggc cagtttatct ggcctgcggt acgtgacggc
     2641 aaggtatcca ttacccgctc gcaactggca atgctcctcg ataagctgga ctggcgtcag
     2701 ccaaaaacat ccagccgtaa ctcactgaca atgttgtaaa aaactcctga ccgcattata
     2761 aaaacggtca tgagtcagaa atacctcatt cgcatcgcag agctggaaag gttgctctct
     2821 gagcaggctg aagccctccg tcagaaagac cagcaactga gtctggttga agagacggaa
     2881 gccttcctgc gctctgcact gacacgtgcc gaagaaaaga tcgaagaaga tgaacgggaa
     2941 atagaacatc tgcgggctca gatagaaaaa ctgcgccgga tgctgttcgg tacccgttct
     3001 gaaaaactgc gtcgtgaagt tgaactggct gaggctctgc tgaaacaacg tgaacaggac
     3061 agcgatcgtt acagtgggcg ggaagacgat cctcaggttc tccgccagtt gcgacagtcg
     3121 cgccatcgtc gtccgttacc ggcacacctt ccccgtgaaa tacaccgcct ggagccagaa
     3181 gaaagctgtt gcccg
//
LOCUS       DAMZFT010000274         3137 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.274, whole genome shotgun sequence.
ACCESSION   DAMZFT010000274 DAMZFT010000000
VERSION     DAMZFT010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3137)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3137)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3137
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.274"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(<1..330)
                     /locus_tag="PN977_005183"
     CDS             complement(<1..330)
                     /locus_tag="PN977_005183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000926344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="morphogenetic protein"
                     /protein_id="HDI6045092.1"
                     /translation="MLLPLFPLPSRPTELIQFRQPNIADAMRFNSITPEEQEQQTTAY
                     LKALLAEPAKYDPLTWTAQDRITALWWIFTGSRETPVETFTYICKHCGKEHYYDCDMN
                     ALAEDIQV"
     gene            complement(345..956)
                     /locus_tag="PN977_005184"
     CDS             complement(345..956)
                     /locus_tag="PN977_005184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000523978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDI6045093.1"
                     /translation="MGLNVASVKSYVSSALTTTLFGSGVGEREVGKLTSIIMNKMLFA
                     QGWQFSVEVDGLEGADFFAKDITYHDYSIEYETIKIGGGNILQPTERSPGQITMMVRD
                     TVDGLVLDWFKTAKGRVINPDGTGNIPSKYLLNVRIYRLLSSGLTKLENEMTVFPVTT
                     GDVTYARDQVTEFKSFPMTFALHSTFNQSSSSLASLLGFSFSL"
     gene            complement(967..1533)
                     /locus_tag="PN977_005185"
     CDS             complement(967..1533)
                     /locus_tag="PN977_005185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000188918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6045094.1"
                     /translation="MTPRQLLEDVKSRFTPLIADEPALLESLLRKALGTYQDRAGHIK
                     RIRITDQASKSLACPVDFLALVSVTDHTGDLVYSDVYDGNIELEDTHRAVYPLNVSYL
                     ANLRDMDLDNGDVPPEIIGLLSDYLEVLIAIPNTDRLRRISIAGKLDASNLSDENTLY
                     QRKLDLEEKMSATRAIIPGIVLFSSMLK"
     gene            complement(1614..2153)
                     /locus_tag="PN977_005186"
     CDS             complement(1614..2153)
                     /locus_tag="PN977_005186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5384 family protein"
                     /protein_id="HDI6045095.1"
                     /translation="MNKIILFLIFSTFSVGTALANSLQSQIAAIAQAENEGRAKEQQA
                     EDARKELIRQQAQAERIRREKAASAAAAREKQRVAAENERRAKREAELANDKKRDQAY
                     EDELRKLQLESMKLELQAKAARVQRENDFIEQELKERAAKTDVIQSEADANRNISTGS
                     KDLLQSEGKAREKKASSWW"
     gene            complement(2157..2669)
                     /locus_tag="PN977_005187"
     CDS             complement(2157..2669)
                     /locus_tag="PN977_005187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000039792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6045096.1"
                     /translation="MSKKYTLCALVVSAILLSGCQSSGADYAADVYDTAQLNSKQETK
                     TVNIISVLPAKVKVDNKANKEAAQTFGAVLGAVAGGVAGYNVKGTSTLGAVAGGTGGA
                     ALGAAAGSLVSDKTIVEGVSLTYKEGTKVFTSTQVGKACQFTTGLAVLISTKDNETRI
                     QPNATCPEKK"
BASE COUNT          836 a          750 c          623 g          928 t
ORIGIN      
        1 gacctggata tcttcagcca gagcattcat atcgcaatcg taataatgct ctttaccgca
       61 atgtttacag atgtaggtga atgtctcgac cggtgtttca cgggagccag taaatatcca
      121 ccataacgcg gtaatccggt cctgcgccgt ccatgtcagg ggatcatatt tcgcgggttc
      181 agccagcaag gcttttaaat acgccgttgt ctgttgttct tgttcctccg gtgttatcga
      241 gttgaaacgc atcgcatcag caatatttgg ctgacggaac tggatcaatt cagttggccg
      301 cgatggtagc gggaaaagag gtaaaagcat ccttgctcct taattcaaag agaaaaacta
      361 aagcccagaa gggaagctaa agaacttgag gattggttaa acgtgctgtg caatgcgaag
      421 gtcattggga atgacttaaa ttccgtaacc tgatcccgcg cataggtgac atcgccggta
      481 gtgaccggga ataccgtcat ctcattttcc agtttggtta aaccggagga cagcaaccga
      541 taaatacgca cattgagcaa atatttagac ggtatattcc cggtaccgtc cggattgatc
      601 acccgacctt ttgccgtctt aaaccagtcc aaaacgaggc catcaacggt atccctgacc
      661 atcattgtta tctgcccagg cgaacgctcc gttggctgaa ggatattccc tccgccgatt
      721 ttaatcgttt catattcgat gctgtaatcg tggtaggtaa tgtctttagc aaagaagtct
      781 gccccctcca gaccatcaac ttcgacagag aactgccatc cttgcgcgaa cagcattttg
      841 ttcatgatga ttgacgtcag cttaccaact tcccgctcac caacgccgga gccaaataat
      901 gtcgtcgtta atgccgaaga tacataagac tttactgaag caacattaag ccccatatca
      961 gccccctcac ttcaacatgg atgagaaaag aacaattccc gggataattg cccttgttgc
     1021 gctcattttc tcttccagat ccagctttcg ctgatacagc gtgttctcgt cggataaatt
     1081 actggcatcg agtttccccg cgatagatat tcttcgcagg cgatcagtgt taggtatcgc
     1141 gattagcact tccagatagt cagaaagtaa cccaattatt tcaggtggca catccccatt
     1201 atccagatcc atatcacgca aattagccag atatgacaca ttcagtgggt ataccgctcg
     1261 atgggtatct tcaagctcga tattcccatc gtaaacatcg gagtagacaa gatcgccggt
     1321 gtgatctgta accgatacga gcgcaagaaa atcaactggg caagcaagtg atttactggc
     1381 ctgatcggtg atgcgtatcc gcttgatgtg ccccgcccta tcctggtagg ttcccaatgc
     1441 ttttcttagc agggattcca gtaaggcagg ttcatccgca atcaaaggtg tgaagcggga
     1501 tttgacgtct tcgagtaatt gtcgtggtgt cattgaaacc tcgtagaatc tagtttgtta
     1561 acagattcta cgagttgtca tttgtgacag tatgctcgca atcgtagtga ttactaccac
     1621 caactgctag ctttcttctc tctagctttt ccttcgcttt gcagtaaatc tttacttcct
     1681 gtagaaatat ttctatttgc atcagcctca gactgaatta catctgtctt agctgctctc
     1741 tccttcaact cctgctctat aaaatcgttt tctcgctgga cacgagccgc ttttgcttgc
     1801 agttcgagtt tcatgctttc gagttgcagc ttgcgaagct catcctcata agcttgatct
     1861 cgcttcttgt catttgctag ctcggcttct cgtttcgctc tacgttcatt ttctgcagca
     1921 acacgctgtt tttcgcgtgc agcagcggca gatgctgctt tttctcttct aatacgttca
     1981 gcttgtgctt gttggcgaat aagttctttt ctggcatcct cagcttgctg ctctttagct
     2041 cgcccttcat tttccgcttg agcaatagca gcaatttgac tttgcaacga gttagccaag
     2101 gcagtgccta cactaaacgt agaaaagatt agaaataaaa taattttatt cataaattat
     2161 ttcttttctg gacaagtagc atttggctga attcgagttt cattatcttt agttgaaata
     2221 agcacagcaa gccccgttgt aaactggcat gctttaccaa cttgagttga agtgaacacc
     2281 ttagttcctt ctttataagt cagtgaaaca ccttcaacaa ttgttttatc actaaccaaa
     2341 gaaccagccg ctgcaccaag agccgcacca ccagtaccgc cagctacagc acccagtgtc
     2401 gatgtgcctt taacattata accggcaacg cccccagcaa cagctcccag taccgcacca
     2461 aatgtctgtg ccgcttcttt attagcttta ttatcaactt tcactttagc tggaagcaca
     2521 gagataatat tgactgtctt tgtttcctgc ttcgaattca attgagcggt atcataaaca
     2581 tcggcagcat aatcagcacc gctagattga cacccagaaa gaagaattgc agatacaaca
     2641 agagcgcata aagtatactt tttagacata agaatcatcc cagaaaaaca aaaaccggcg
     2701 agaagttacc acgagaacgg aaagtaaaca agaatactcc cattgactta tatcaattaa
     2761 tcaacttttt ataagctatc cctaacttat tgtgtatagc tgaaaagtca gagacaagtt
     2821 caccatctgc attatttctt aagttaatag tttattgcta acttaattta tgtatcgact
     2881 attgatatcc atcttaaagt tacaatatcc ctctgcatta tttttcatca caaataatat
     2941 atctattgca cgacgctaat ctgctctaat aaatttgtat ttttaagtcg cgaatgctat
     3001 ctttttgcat catattgacc ttttaatcgt tcaggcttat agtttcgccg tcgtagcaaa
     3061 ttctgcgacc gggtttagca gcctgaatga ttgtgcggac agccgcagat atccgatatt
     3121 gcggtatttt tgtgtcc
//
LOCUS       DAMZFT010000275         3076 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.275, whole genome shotgun sequence.
ACCESSION   DAMZFT010000275 DAMZFT010000000
VERSION     DAMZFT010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3076)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3076)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3076
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.275"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(<1..742)
                     /locus_tag="PN977_005188"
     CDS             complement(<1..742)
                     /locus_tag="PN977_005188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001166084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03752 family integrating conjugative element
                     protein"
                     /protein_id="HDI6045097.1"
                     /translation="MQIKSNTLVKFVVPAVVLAGLVITMKSCSTPESGSESTAQSGAL
                     AGLSSEELRALGVEGDTPEDTLRTLVARLNQVRDNQKTLESQNAQLLKDNEQLRRRGT
                     DVSGQVSEAVAGMQKTYDQKQRELQEQQRSLMSQIDVLRGQLSDQLTGSHTKLSDIPD
                     GLGLDSGSGAGSLISQGADGLMWVSPADQSQDDPLSSGGKSGPRFPTSFLEDNPLTRQ
                     KAAYEQQVKGYSNEQQAATTQAVYTLPEN"
     gene            complement(732..1652)
                     /locus_tag="PN977_005189"
     CDS             complement(732..1652)
                     /locus_tag="PN977_005189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001258328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03749 family integrating conjugative element
                     protein"
                     /protein_id="HDI6045098.1"
                     /translation="MRTCVLRNLLRPAAVMLSLSVMSLTAQADELMKWERIPLDVPLM
                     VGQERVIFADKNVRVGFPPALNGRLRVQSTGGAVYLKADSAFPKTRIQLQDVESGEMI
                     LLDVTAAEKGPTEPVRIVYDGEVNTVSRAGGSAPESAKAGSSDGRPGKVGADDGTQAR
                     RTAPRYDEPLPVLLTRYAAQSLYAPLRTLEPVAGIRPVVPPLPRQITTLYPSEPLTVS
                     PVAAWSAGGRTVVALKLTSTVSRRIVLDPRALQGRFVAATFQHRWLGAAGTPEDTTML
                     YVVTAGRPEKAFVAEPVAPRRTGGEVKHAD"
     gene            complement(1649..2296)
                     /locus_tag="PN977_005190"
     CDS             complement(1649..2296)
                     /locus_tag="PN977_005190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001696694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03746 family integrating conjugative element
                     protein"
                     /protein_id="HDI6045099.1"
                     /translation="MSRFRHALQDRDQHILTLRVFSGVLAGLLLMVTLGWMSAPDRLT
                     IHNPPDLRSGSTRPWWEVPPSTVYSFAYYIFQQLNAWPKNGEVDYPAKIHALSAYLTP
                     SCREILETDARRRKDAGELLDRVRVVYEIPEHGYGGRYNRNPVTVEGRDNWQVRLDLV
                     VDEYFHAEPVKRALVRYPMKVVRMEGDPELNPFALALDCYSSTPQRLEAVKGGGE"
     gene            complement(2293..2658)
                     /locus_tag="PN977_005191"
     CDS             complement(2293..2658)
                     /locus_tag="PN977_005191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03750 family conjugal transfer protein"
                     /protein_id="HDI6045100.1"
                     /translation="METIRFLPDRLNAEPTVFRGFTTPELGLAALSGVFLGLLWTLPL
                     IPLFGWVIIPTGALLTPLLLVWTGGVWITRLKRGKPDNWLWQRIEEKKAALGVGRPAF
                     IREAQGWSLRRSRVRGGKG"
     gene            complement(2678..3043)
                     /locus_tag="PN977_005192"
     CDS             complement(2678..3043)
                     /locus_tag="PN977_005192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000475734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03745 family integrating conjugative element
                     membrane protein"
                     /protein_id="HDI6045101.1"
                     /translation="MFKNAFTFRHLRLAGMGLIAFLLGSLTPALADLPKIEPPSSGGG
                     GGFFNQLKGYIQDGIVLFGLVVAAAAFVKVAEAAVTTFSEVRDGKTGWGQFGAIIVVG
                     VVLLVAVIWLLGKSANIIL"
BASE COUNT          644 a         1036 c          810 g          586 t
ORIGIN      
        1 agttctccgg cagggtgtac acggcctgcg tggtggccgc ctgctgctca ttgctgtacc
       61 ccttcacctg ctgctcatag gctgctttct gccgggtcag ggggttatct tcaaggaagg
      121 aggtcggaaa acgcggaccg ctctttccgc cgctgctcag cggatcatcc tgtgactgat
      181 cggccggact cacccacatc agcccgtccg ccccctgcga gatcaacgac ccggcaccac
      241 tgcctgaatc cagtcccagc ccgtcaggaa tatcgctgag cttcgtgtga cttcccgtca
      301 gctggtcact cagttgcccc cgcagcacat caatctgcga catcagggag cgctgctgct
      361 cctgcagttc acgctgcttc tgatcgtagg ttttctgcat tcccgccacg gcttcactga
      421 cctggccgga cacatccgtc ccccggcgac gcagctgttc gttgtccttc agcaactgcg
      481 cattctggct ctccagcgtt ttctggttat ccctgacctg gttcaggcgg gcaaccaggg
      541 tccgcagggt atcctccggc gtgtcccctt ccacgcccag cgcacgcagc tcttctgacg
      601 ataatccggc cagcgcaccg ctctgtgctg tactctcact cccgctctcc ggtgtgctgc
      661 agcttttcat cgtgatcacc agccccgcca gtaccacagc aggtaccaca aatttcacca
      721 gggtattgga cttaatctgc atgtttcacc tccccgcccg tcctgcgcgg tgccacaggc
      781 tctgccacaa aggccttctc cgggcggcct gccgtcacca catacagcat tgtggtgtct
      841 tccggtgtcc cggcagcacc cagccagcgg tgctggaagg tggccgccac aaaacggccc
      901 tgcagggctc gcggatcaag cacaatacgc cggctcaccg tactggtgag tttcagggcc
      961 accacggttc gtccgccggc ggaccatgcc gccaccgggg ataccgtcag gggttcagac
     1021 gggtaaagcg tggtgatttg ccggggaaga ggtggcacca ccggtctgat ccccgcaacc
     1081 ggttcaaggg tgcgcagcgg agcatacagg gactgtgccg cataccgggt cagcagaacc
     1141 ggcaagggct cgtcataacg gggggcggtc cggcgggcct gcgtgccgtc atccgcaccg
     1201 actttcccgg gacgtccgtc actgctgccc gccttcgcgc tctccggagc gctgccgccg
     1261 gcacggctca ccgtgttcac ctccccgtca tacacaatcc ggacaggctc agtgggcccc
     1321 ttttctgcgg ccgtcacatc cagcaggatc atctccccgc tttccacatc ctgcagctgg
     1381 atccgcgttt tcggaaacgc gctgtccgct ttcaggtaaa cggcaccgcc ggtgctctgc
     1441 acccgcagcc tgccgttcag tgccggcgga aagcccaccc tcacgttttt atcggcaaaa
     1501 atgacccgct cctgccccac catcagcggg acatccagcg gaatacgctc ccacttcatc
     1561 agttcatctg cctgcgccgt cagggacatc actgacagcg acaacatcac agcggcagga
     1621 cgcagcaggt tgcgcagtac acaggttctc attcaccacc tcccttcacg gcttccagac
     1681 gctgcggcgt ggagctgtaa cagtccagcg ccagagcaaa cggattaagt tccggatcgc
     1741 cctccatacg caccaccttc atcggatagc ggaccagcgc ccgtttgacc ggctcagcat
     1801 gaaaatactc atccaccacc aggtcgagac gaacctgcca gttgtcccgg ccttccaccg
     1861 tgaccggatt acggttgtaa cgtccgccgt aaccgtgctc agggatctca tacaccacac
     1921 gaacacgatc cagcagttcc cccgcgtcct tacggcgtcg tgcatccgtt tcaaggattt
     1981 cccggcacga cggtgtcaga taggccgaca gggcatgaat ttttgccgga taatccacct
     2041 caccgttttt cggccaggca ttcagttgct ggaaaatgta atacgcaaag gaatagacag
     2101 tggaaggcgg aacctcccac cacggacggg tactgccgga acgtaaatca ggtggattat
     2161 ggatggtcag ccggtcaggt gcagacatcc agccgagggt gaccatcaga agcagccccg
     2221 ccagcacacc actgaacaca cgaagcgtca gaatatgctg gtcacgatcc tgcagggcat
     2281 gacgaaaacg actcatcctt ttcctcccct gacgcgggaa cgacgcaggg accagccctg
     2341 tgcttccctg ataaatgcag ggcgccccac acccagcgcc gcttttttct cttcaatacg
     2401 ctgccagagc cagttatccg gtttgccccg tttcagccgg gtgatccaca cgccgccggt
     2461 ccagaccaga agcagtggag tgagcagggc accggtggga ataatcaccc agccgaacag
     2521 cggtatcagt ggcagtgtcc agagaagacc gagaaagaca ccgctcagtg cggccagacc
     2581 cagctctggc gtggtgaatc cccggaacac cgtcggttcg gcattcagac gatcgggcag
     2641 aaaacgaatg gtttccataa acagccctcc tggtggatta caggatgatg ttcgcggatt
     2701 tgccgagcag ccagataacc gcaaccagca gcaccacccc gaccacgata atggccccga
     2761 actgtcccca gcccgtcttg ccgtcacgca cctcagaaaa agtagtaacg gcagcctccg
     2821 ccaccttcac aaaggccgcc gctgcaacta ccagaccaaa cagcacaatg ccatcctgga
     2881 tatagccttt gagttgatta aaaaaaccac cacccccacc ggatgatggc ggctcaattt
     2941 tgggcaaatc ggccagtgcc ggggtcagac tgcccagcag aaaagcaatc agccccattc
     3001 ccgccagacg caggtgacgg aaagtaaaag cgtttttaaa catatatgtc tccttaaaga
     3061 aaacggaaaa tcagcc
//
LOCUS       DAMZFT010000276         3074 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.276, whole genome shotgun sequence.
ACCESSION   DAMZFT010000276 DAMZFT010000000
VERSION     DAMZFT010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3074)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3074)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3074
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.276"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..1076
                     /locus_tag="PN977_005193"
     CDS             <1..1076
                     /locus_tag="PN977_005193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416342.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDI6045102.1"
                     /translation="SDSLQMLTLARVIQGFGGAALMSVNTALIRLIYPQRFLGRGMGI
                     NSFIVAVSSAAGPTIAAAILSIASWKWLFLINVPLGIIALLLAMRFLPPNGSRASKPR
                     FDLPSAVMNALTFGLLITALSGFAQRQSLTLIGAELVVMVVVGIFFIRRQLSLPVPLL
                     PVDLLRIPLFSLSICTSVCSFCAQMLAMVSLPFYLQTVLGRSEVETGLLLTPWPLATM
                     VMAPLAGYLIERVHAGLLGALGLFIMAAGLFSLVLLPASPADINIIWPMILCGAGFGL
                     FQSPNNHTIITSAPRERSGGASGMLGTARLLGQSSGAALVALMLNQFGDNGTHVSLMA
                     AAILAVIAACVSGLRITQPRSMA"
     gene            complement(1122..2003)
                     /gene="htpX"
                     /locus_tag="PN977_005194"
     CDS             complement(1122..2003)
                     /gene="htpX"
                     /locus_tag="PN977_005194"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014229823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HDI6045103.1"
                     /translation="MMRIALFLLTNLAVMVVFGLVLSLTGIQSSSVQGLMIMALLFGF
                     GGSFVSLLMSKWMALRSVGGEVIEQPRNERERWLVNTVATQARQAGIAMPQVAIYHAP
                     DINAFATGARRDASLVAVSTGLLQNMSPDEAEAVIAHEISHIANGDMVTMTLIQGVVN
                     TFVIFISRILAQLAAGFMGGNRDEGEESNGNPLIYFAVATVLELVFGILASIITMWFS
                     RHREFHADAGSAKLVGREKMIAALQRLKTSYEPQEATSMMAFCINGKSKSLSELFMTH
                     PPLDKRIEALRTGEYLK"
     gene            complement(2195..>3074)
                     /locus_tag="PN977_005195"
     CDS             complement(2195..>3074)
                     /locus_tag="PN977_005195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="carboxy terminal-processing peptidase"
                     /protein_id="HDI6045104.1"
                     /translation="DLRSNGGGALTEAVSLSGLFIPSGPIVQVRDNNGKVREDSDTDG
                     QVFYKGPLVVLVDRFSASASEIFAAAMQDYGRALVVGEPTFGKGTVQQYRSLNRIYDQ
                     MLRPEWPALGSVQYTIQKFYRVNGGSTQRKGVTPDIIMPTGNEETETGEKFEDNALPW
                     DSIDAATYVKSGDLTAFEPELLKEHNARIAKDPEFQNIMKDIARFNAMKDKRNIVSLN
                     YAVREKENNEDDATRLARLNERFKREGKPELKKLDDLPKDYQEPDPYLDETVNIALDL
                     AKLEKARPAEQPAPVK"
BASE COUNT          677 a          828 c          730 g          839 t
ORIGIN      
        1 tttctgattc gctgcaaatg ctcacccttg cgcgtgtcat acaaggtttc ggcggtgcag
       61 cgttgatgag cgttaatacc gcacttatcc gcctgatcta tccacaacgt tttctgggta
      121 gagggatggg cataaactcg tttattgttg ccgtctcttc tgctgccggg ccgacaattg
      181 ctgcagcaat cctctccatc gcatcctgga aatggttatt tttaatcaac gtaccgttgg
      241 gtattatcgc cctgcttctg gcgatgcgtt ttctgccacc caatggttct cgcgccagta
      301 aaccccgttt cgacctgccc agcgccgtga tgaacgcgtt aaccttcggc ctgcttatta
      361 ctgcattgag tggtttcgct cagaggcaat cgctgacatt gattggtgcg gaactggtgg
      421 taatggttgt cgttggtatt ttctttattc gccgccagct ttctcttccc gtaccgctgc
      481 taccggtgga tttactgcgt atcccgctgt tttcactttc tatttgcaca tctgtttgct
      541 ctttctgcgc acaaatgctg gcaatggttt cccttccctt ttacctgcaa accgtgctcg
      601 ggcgtagtga agtcgaaaca ggtttacttc tgacaccgtg gccgttagca acaatggtga
      661 tggctccact ggcaggctat ttgattgaac gcgtacatgc aggattgctg ggtgctttag
      721 ggttattcat catggctgcg gggctttttt ccctggttct gctgccagcg tcacctgcgg
      781 atatcaatat tatctggccg atgatcttat gtggtgctgg atttggcttg ttccagtcac
      841 ccaataacca caccattatt acctccgctc ctcgcgaacg tagcggtgga gccagtggca
      901 tgttagggac ggctcgtctt ctgggtcaga gtagcggcgc ggctctggta gcgctgatgc
      961 taaatcagtt tggtgataat ggtacgcacg tctcgctgat ggctgcggct attctggcgg
     1021 tgattgcagc ctgtgtcagt ggtttacgta tcactcagcc acgatccatg gcataaaaaa
     1081 agcgcgtcga tcaggacgcg ctttttagta tttacttcat attacttcag gtattcaccc
     1141 gtacgcagag cttcaatacg tttatccagt ggcgggtgag tcatgaacaa ctcactgagc
     1201 gatttcgact tcccgttaat gcagaaagcc atcatgctgg ttgcttcttg cggttcatag
     1261 ctggttttca ggcgttgcaa tgcagcaatc attttctcgc gaccaaccag ttttgctgaa
     1321 cccgcatcgg cgtggaattc acgatgacgc gagaaccaca tggtgataat gctcgccaga
     1381 ataccaaaca ccagttccag aaccgttgca accgcaaagt agatcagcgg gttgccgttg
     1441 ctctcttcac cttcatcacg atttccgccc ataaaacccg cggcaagttg cgccagaata
     1501 cgggaaataa agataacgaa ggtgttcacc acgccctgaa tcagcgtcat ggtgaccata
     1561 tcaccattgg cgatgtggct gatttcgtga gcaattaccg cctcggcttc atccgggctc
     1621 atgttctgca gcaaaccagt gctgacagca accagagaag catcacggcg cgcaccggtt
     1681 gcaaaagcat tgatgtccgg cgcatggtag atagccactt gcggcatagc gatccccgcc
     1741 tgacgagcct gggttgctac agtattaacc agccaacgtt ccctttcgtt acgcggttgc
     1801 tcgatcactt ccccgccaac agatcgtaat gccatccatt tggacatcag aagcgaaacg
     1861 aaggaaccac caaaaccgaa cagcaaggcc atgatcatca gcccctgaac gctgctcgac
     1921 tgtatccctg tcaggctcag taccagcccg aaaacgacca ttacggccag gttcgttagc
     1981 aggaagagcg cgattcgcat cataattttc tttttacctc agtttaacaa aacgcaatat
     2041 gcgataccca catcgtatgg gttacgcgac tattttcaag tctggatagt gcgtaagtca
     2101 ccagaaagac acaactttac attttgtagc atctgattta cggcatcttg ccgctgttaa
     2161 aaaatcaggc acaatttctt gtgcctgatt gatgttactt gacgggagcg ggttgttccg
     2221 cgggtctggc tttttcaagc ttcgccagat cgagtgcgat attcaccgtc tcatccagat
     2281 aaggatctgg ctcctggtaa tctttcggta gatcatccag tttcttcaac tccggtttac
     2341 cttcgcgttt aaagcgttcg ttcaaacgcg ccagacgcgt cgcatcatct tcattattct
     2401 ctttctcacg cacagcgtaa ttcagagaaa cgatattgcg cttgtccttc atagcgttga
     2461 agcgtgcgat atccttcatg atgttctgga actcaggatc tttcgcgata cgcgcattat
     2521 gttccttcag cagctccggt tcaaaggccg ttaaatctcc tgatttcaca taagtcgcgg
     2581 catcaatgct atcccacggc agcgcgttat cttcgaattt ctcacccgtt tccgtttctt
     2641 cattacccgt cggcatgatg atgtctggcg ttacgccttt acgttgcgta ctgccgccgt
     2701 taacgcgata gaatttctgg atcgtgtact gcacagatcc cagcgctggc cattcaggac
     2761 gtaacatctg atcgtagata cggttcaatg aacggtattg ctgaacggtg cctttaccaa
     2821 acgtcggttc acccacaacc agcgcacgac cgtaatcctg cattgccgcg gcaaagattt
     2881 ctgaagccga agcactgaaa cggtcaacca gtaccaccag cgggccttta tagaagacct
     2941 gtccgtcggt atcgctgtct tcacgaactt taccgttgtt atcacggacc tgaacaatcg
     3001 ggccggatgg aataaacaga ccggagagcg ataccgcttc ggtcagcgcc ccaccaccat
     3061 tgctacgcag gtcg
//
LOCUS       DAMZFT010000277         3070 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.277, whole genome shotgun sequence.
ACCESSION   DAMZFT010000277 DAMZFT010000000
VERSION     DAMZFT010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3070)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3070)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3070
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.277"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..306
                     /locus_tag="PN977_005196"
     CDS             <1..306
                     /locus_tag="PN977_005196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein"
                     /protein_id="HDI6045105.1"
                     /translation="NQLLDILRHKALTQMAQESGGSATVRLNTLDWLGGQGREQADNE
                     WHDAINWLGDWCSEEQHPVIWSTTQAAEHLPVRMPRLCSAERLSESMVDEIFQKGAA"
     gene            303..1373
                     /locus_tag="PN977_005197"
     CDS             303..1373
                     /locus_tag="PN977_005197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HDI6045106.1"
                     /translation="MSTEMKTGLVLSGGGAVGAYQAGVVKALAECGTQISMVSGASIG
                     AFNGAIIAASTDLSEAAVRLEALWDHLGNNQVLSVNRLVYFSLLKKLFQAMNLCQIPG
                     RAGALLTTLLRHISTINGFDNLMAQPLLSDEPLTALMDHYLDTDALADGLPLYVSLYP
                     TEGGMQDIIDCIRAELGVGTTKNAVFQHIQSLPRGQQKEALLASAALPLLFRPREVQG
                     TMFGDGGMGGWRNMQGNTPVTPLVDAGCNMVIVTHLSDGSLWDRQAFPDTTILEIRPR
                     KRLKYAGDGGNSGGLLSFTSAHTDAWRQQGYEDTMLAMEHIRKPLAARQALTRSEAVL
                     QKSLDITEEADLALRNAMARIK"
     gene            1509..2192
                     /locus_tag="PN977_005198"
     CDS             1509..2192
                     /locus_tag="PN977_005198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6045107.1"
                     /translation="MKLPVKLLMSLISLVSVIARAGEYKNYSRDEIKYWRYTSYKGGK
                     LPEGFTDEKFSSAIYNGRIFTMKRLHTLMLFLAVLFTGFNFNAEAATVKQALSCNPEA
                     WAEQPGACPSTYELYEGDATYKAAIDKALKPVGLSGMFGKGGYMDGPGGGITPVNING
                     TVWFQGDGCKANTCGWDFIVTLYNPKTHEVVGYRYFGLDDPAYLVWFGEIGVHEFAYL
                     VKNYVAAVN"
     gene            2208..2618
                     /locus_tag="PN977_005199"
     CDS             2208..2618
                     /locus_tag="PN977_005199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6045108.1"
                     /translation="MKTFIKTLLVAVTILFSVFATAKQVKLPNNIKYVNTTEAFSCTE
                     IDGMNCQTKNQFNYKDNSYVFVLERGGAWCYDYTVSVVNLKTGKAQMIEYGDNQLCSG
                     SNKPFFEIKNGVPTVGVIDTSGKPVVVAQDKLKI"
     gene            2839..>3070
                     /locus_tag="PN977_005200"
     CDS             2839..>3070
                     /locus_tag="PN977_005200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF932 domain-containing protein"
                     /protein_id="HDI6045109.1"
                     /translation="MRLASRFGYAANQIRRDRPLTHEELIRHVPSIFGEDRHISRSER
                     YAYIPTITVLENLQREGFQPFFACQTRVRDPGR"
BASE COUNT          801 a          723 c          804 g          742 t
ORIGIN      
        1 aaccagctac tggatattct cagacataaa gcattaacgc agatggcaca ggagagtggc
       61 ggtagtgcaa ccgtgcgtct gaacactctg gactggcttg gtggtcaggg ccgggagcag
      121 gcagataacg agtggcatga cgcgattaac tggctgggag actggtgcag tgaagagcag
      181 catccggtga tctggtcaac cacgcaggct gcagaacact taccggttcg catgcctcgc
      241 ctctgctctg cagaacgcct ctctgaaagt atggtcgatg aaatatttca gaagggggct
      301 gcatgagtac agaaatgaaa acggggctgg tgctgtccgg tggcggggcg gtgggcgctt
      361 atcaggcggg agtggttaag gcactggcag agtgtggtac acagatcagc atggtttcag
      421 gggccagcat tggcgcattc aatggtgcca ttatcgcggc ctctaccgat ctgtcagaag
      481 ctgccgtacg cctggaggcg ctctgggatc atctggggaa taatcaggtg ctgtcggtaa
      541 acagattggt ttacttttca ttgctgaaaa aattgttcca ggcaatgaac ctctgccaga
      601 tccccggacg tgcaggagca ctgcttacga cgcttcttcg ccatatatcg acaatcaacg
      661 ggtttgacaa tctgatggct cagccgttgt tgtcagatga gcccctgaca gcgctgatgg
      721 atcattatct tgatactgat gctctggcag acgggctacc gctgtatgtg tcgctgtacc
      781 ccacagaagg gggcatgcag gatattattg actgcattcg tgctgaactg ggtgtcggaa
      841 ccacgaaaaa cgccgttttt cagcatatcc agagcctgcc ccgcggacag cagaaagagg
      901 ctctgcttgc gtcagccgcg ctgcccctgc tgttccgtcc ccgtgaggtt caggggacaa
      961 tgttcggtga tggtggtatg ggaggatggc gaaatatgca gggaaatacc cctgtgacgc
     1021 ctctggtcga tgccggatgc aatatggtga ttgtgacgca tctgagtgac ggttctttat
     1081 gggatcgcca ggcttttccg gacaccacaa tccttgagat ccgtccccgg aaaaggctga
     1141 aatatgcagg tgatggtggc aacagcggcg gtctgctcag ttttacatcg gcacataccg
     1201 acgcctggcg tcagcagggc tatgaagaca cgatgctggc gatggagcat atccggaaac
     1261 cgctggcagc acgtcaggca ctgacccggt cagaggcggt attgcagaaa agcctggata
     1321 taacggaaga ggcagatttg gcactgagaa acgcgatggc ccggattaaa taaagacgcc
     1381 ccaaagaaac gccaccgcct gtggtggtgt ttttcctgtc tggtgttatt gaaaatattt
     1441 tcagatatat attacctgag agaaagtttt acccctcacc tgtcaattca ctcactggaa
     1501 atttaattat gaaattacct gtaaagttat taatgagcct tatatctctg gtcagcgtta
     1561 ttgcacgtgc cggggaatat aaaaattaca gccgggatga aataaaatac tggcgatata
     1621 catcatacaa gggggggaaa cttccggaag gtttcactga tgagaaattt tccagcgcca
     1681 tttacaacgg aagaatattt acaatgaaac gtttacatac ccttatgtta tttctggcgg
     1741 ttctgttcac gggctttaac tttaacgcag aagcggctac cgtaaaacaa gctcttagtt
     1801 gtaacccgga agcctgggct gaacaacctg gagcgtgtcc atcaacgtac gagttgtacg
     1861 aaggtgacgc tacctacaaa gccgctatcg acaaagcatt aaaaccggtc ggactgagcg
     1921 gcatgttcgg taaaggcggc tatatggatg gccctggtgg aggtatcacg ccagtaaaca
     1981 ttaacggtac agtctggttc cagggcgacg gttgcaaagc caatacctgc ggctgggact
     2041 ttatcgtaac actctataac ccaaaaaccc atgaagttgt tggctaccgt tactttggtt
     2101 tagatgaccc ggcctacctg gtttggttcg gtgaaattgg cgtgcatgaa ttcgcgtatc
     2161 tggtgaaaaa ctacgtagct gcggttaact aatttaagga gtctatcatg aaaactttta
     2221 tcaaaacttt actcgttgct gtaactattc tgttctctgt cttcgctact gcgaaacaag
     2281 taaaactgcc aaataacatc aaatacgtta atactacaga agcgttttcc tgtaccgaaa
     2341 ttgacggtat gaattgccag acaaagaatc agttcaacta taaagataac agctatgttt
     2401 tcgtgcttga gcgtggtggt gcctggtgct acgactacac tgtctcggta gttaacctga
     2461 aaaccgggaa agcacaaatg attgaatacg gggacaatca actgtgctca ggtagcaaca
     2521 aaccgttctt cgaaatcaaa aatggcgtac cgacggtagg agtcatcgac acatccggaa
     2581 aacctgtcgt tgtagcccag gacaaactta aaatctaagg agtaaaccat gcgtttctct
     2641 gcacattatt aatatcaaag gtaaatacag ccccagccat tgttaccgga aagccgctta
     2701 atgcgtggct cgccagtgaa aggcctctca gacaataaac cttattcatt tcacccgtca
     2761 ggccgcgtct tctccgggag acgcggcttt tttcatttat tgcaccagta aatcttaacc
     2821 accgataagg agcaaagtat gcgattagcc agtcgttttg gttatgctgc aaaccagata
     2881 cgccgtgacc gtccgctgac acatgaagaa ctgatacgcc atgtacccag tatttttggg
     2941 gaagaccggc acatctcccg cagtgaacgg tatgcgtaca ttcccaccat taccgtcctg
     3001 gaaaatctgc agcgggaagg ctttcagccg ttcttcgcct gccagacccg tgtgcgcgac
     3061 ccgggccgcc
//
LOCUS       DAMZFT010000278         3063 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.278, whole genome shotgun sequence.
ACCESSION   DAMZFT010000278 DAMZFT010000000
VERSION     DAMZFT010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3063)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3063)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3063
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.278"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..194
                     /locus_tag="PN977_005201"
     CDS             <1..194
                     /locus_tag="PN977_005201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001468473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GTPase family protein"
                     /protein_id="HDI6045110.1"
                     /translation="LMTALPDHAASPLMTRLQDELRTESVRSQAREQFTGAVDRIFDT
                     AESVCVATVARAVWNWIFF"
     gene            630..1010
                     /locus_tag="PN977_005202"
     CDS             630..1010
                     /locus_tag="PN977_005202"
                     /inference="COORDINATES: protein motif:HMM:NF013677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence hypothetical
                     protein"
                     /protein_id="HDI6045111.1"
                     /translation="MQKNVTPGRRKGCPNYPPEFKQQLVAASCEPGISISKLALENGI
                     NANLLFKWRQQWREGKLLLPSSESPQLLPVTLDAAAEQPESLAEDPETLSISCEVTFR
                     HGTLRFNGNVSEKLLTLLIQELKR"
     gene            1007..1354
                     /gene="tnpB"
                     /locus_tag="PN977_005203"
     CDS             1007..1354
                     /gene="tnpB"
                     /locus_tag="PN977_005203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019104956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HDI6045112.1"
                     /translation="MIPLPSGTKIWLVAGITDMRNGFNGLAAKVQTALKDDPMSGHVF
                     IFRGRSGSQVKLLWSTGDGLCLLTKRLERGRFAWPSARDGKVFLTQVQLAMLLEGIDW
                     RQPKRLLTSLTML"
     gene            1404..2941
                     /locus_tag="PN977_005204"
                     /pseudo
     CDS             1404..2941
                     /locus_tag="PN977_005204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998048.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
BASE COUNT          733 a          828 c          852 g          650 t
ORIGIN      
        1 cgctcatgac agcgcttccc gaccatgccg ccagtcccct gatgacccgc ctgcaggacg
       61 agctgcgcac ggaatctgtc cggtctcagg cccgtgaaca gtttaccggt gcggtggacc
      121 ggatatttga tacagcggag agcgtctgtg ttgccactgt tgcacgcgcg gtatggaact
      181 ggattttctt ctgaaacctg cggtgatgtt cctgtctgct gttacatttt tgtaacccca
      241 tgaaaacatg gtgcaaacag tcatgtatgc aactcattct cttgcacact acctttctgg
      301 ttaatagaat atatcgatcg tagctatttt attgatcgtt tatatcgatc gacaaagcaa
      361 taatcaccct tgtaataacc tgcagaaaga cacggccgtc catgaaattc agggcagaaa
      421 aacaccacca cagcgttagc gcctcacagg ggcgcggaat aacacacatg ccggtatcca
      481 cacactgtgg gtaccggctt tttatttaca tcattgatta gggagaatgt aatgaaggta
      541 agcgtacagc ctgaaccgtc tggtcagaat ctgacgaatt agacaaagtg gtgtccacca
      601 aataagtagt gggaaccaaa gtgtcagata tgcagaaaaa tgtgactccc ggcaggcgaa
      661 aaggctgccc taattatcct cccgaattta aacagcagct cgttgctgcc tcctgtgaac
      721 ccgggatatc catctcaaaa cttgctcttg aaaatggcat taacgccaat ctgttgttca
      781 aatggcgaca acaatggcgc gagggaaagc tgctattacc ttcttcagag agcccccagc
      841 tacttcctgt gactctcgat gcagctgccg aacagccaga atcgctcgca gaggacccgg
      901 aaaccctcag tatcagctgt gaggtaacgt tccggcacgg gacgctccgc ttcaatggca
      961 atgtcagcga aaagctcctg actctgctga tacaggaact gaagcgatga tcccgttacc
     1021 ttccgggacc aaaatttggc tggttgccgg tatcaccgat atgagaaatg gcttcaacgg
     1081 cctggctgcg aaagtacaga cggcgctgaa agacgatccc atgtccggcc atgttttcat
     1141 tttccggggc cgcagcggca gtcaggttaa actgctgtgg tccaccggtg acggactgtg
     1201 cctcctgacc aaacggctgg agcgtgggcg cttcgcctgg ccgtcagccc gtgatggcaa
     1261 agtgttcctt acacaggtgc agctggcgat gctgctggaa ggtatcgact ggcgacagcc
     1321 taagcggctg ctgacctccc tgaccatgct gtaaatctct ttatcctggt tgtcacagaa
     1381 taagcccggt aaaatacggg cttatgaacg acatctcttc tgacgacatc ttcctgctga
     1441 aacagcgcct ggccgaacag gaagcgctga tccacgccct gcaggaaaag ctgagcaacc
     1501 gggagcgcga aatagaccat ctgcaggcgc agctggataa actccgccgg atgaacttcg
     1561 gcagtcgttc cgaaaaagtc tcccgccgta tcgcacaaat ggaagccgat ctgaaccggc
     1621 ttcagaaaga gagcgatacg ctgactggta gggtgtatga cccgcagtac agcgtccgtt
     1681 gcgtcagacc cgcatccgta agccgttccc tgaatcacta ccccgtgacg aaaagcgact
     1741 gttgcctgcg gcgccgtgct gcccgaactg cggcggttca ctgagctatc tgggcgagga
     1801 taccgccgaa cagctggagt tgatgcgtag cgccttccgg gttatccgga cggtacggga
     1861 aaaacatgcc tgtactcagt gcgatgccat cgtgcaggca cctgcacctt cgcggcccat
     1921 cgagcggggt atcgccggac cggggctgct ggcccgcgtg ctgacctcga agtatgcaga
     1981 gcacaccccg ctgtatcgcc agtcagaaat atacggccgg caaggtgtgg agctgagccg
     2041 ttcactgctg tcgggctggg tggatgcatg ctgccggctg ctgtctccgc tggaagaggc
     2101 gcttcatggc tatgtcatga ctgacggcaa actccatgcc gatgataccc cggtccaggt
     2161 actgatgccg ggtaataaga agacgaagac cgggcggttg tgggcgtatg ttcgtgatga
     2221 ccgcaatgcc gggtcagcgt tggcacctgc agtgtggttc gcttacagcc cggacagaaa
     2281 aggcatccat ccgcagactc atcttgcttg cttcagcggt gtgctgcaag cggatgcgta
     2341 cgccgggttc aacgagctgt atcgcaatgg tgggataacg gaagctgcct gctgggctca
     2401 tgcccgccga aagatccacg atgtgcacgt ccgcatcccg tcagcactga cggaagaagc
     2461 cctggagcag atcggtcagt tgtacgccat agaggcggat ataaggggaa tgccggcaga
     2521 gcagcggctt gctgaacgtc agcgaaaaac gaaaccgctg ttgaaatccc tggaaagctg
     2581 gttgcgtgaa aatatgaaga ccctgtcgcg acactcagag ttggcgaagg cgttcgcgta
     2641 cgcaattaac cagtggccgg cactgacgta ctatgcgaac gatggctggg tggaaatcga
     2701 caacaacatc gctgaaaatg ccctgcgggc ggtaagtctg ggtcgtaaaa acttcctgtt
     2761 cttcggctct ggccatggtg gtgagcgggg agcgctactg tacagcctga tagggacgtg
     2821 caaactgaat gacgtggttc cagaaagcta ccttcgccat gtgcttggcg tcatagcaga
     2881 ctggccggtc aaccgggtca gcgaactgct tccgtggcgc atagcactgc cagctgaata
     2941 acacatcccc gtcaatacgg ccctcgctgt acgcttacaa cctgaacgga ctggcaacag
     3001 gagcaatgaa ggttaactgt gaccatgaaa gaaccttaaa agtggctatt atacccctaa
     3061 ttc
//
LOCUS       DAMZFT010000279         3063 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.279, whole genome shotgun sequence.
ACCESSION   DAMZFT010000279 DAMZFT010000000
VERSION     DAMZFT010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3063)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3063)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3063
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.279"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..657
                     /locus_tag="PN977_005205"
     CDS             <1..657
                     /locus_tag="PN977_005205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine N-succinyltransferase"
                     /protein_id="HDI6045113.1"
                     /translation="LDPDWRKEGNGYLLSKSRFMFMAAFRDKFNDKVVAEMRGVIDEH
                     GYSPFWQSLGKRFFSMDFSRADFLCGTGQKAFIAELMPKHPIYTHFLSQEAQDVIGQV
                     HPQTAPARAVLEKEGFRYRNYIDIFDGGPTLECDIDRVRAIRKSRLVEVAEGQPAQGD
                     FPACLVANENYHHFRVVLVRTDPATERLILTAAQLDALKCHAGDRVRLVRLCAEEKTA
                     "
     gene            654..2132
                     /gene="astD"
                     /locus_tag="PN977_005206"
     CDS             654..2132
                     /gene="astD"
                     /locus_tag="PN977_005206"
                     /EC_number="1.2.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate-semialdehyde dehydrogenase"
                     /protein_id="HDI6045114.1"
                     /translation="MTLWINGDWITGQGASRVKRNPVSGEVLWQGNDADAAQVGLACR
                     AARAAFPRWARLSFGDRQVRVERFAALLESNKAELTAIIARETGKPRWEAATEVTAMI
                     NKIAISIKAYHVRTGEQRSEMPDGAASLRHRPHGVLAVFGPYNFPGHLPNGHIVPALL
                     AGNTIIFKPSELTPWSGEAVMRLWQQAGLPPGVLNLVQGGRETGQALSALEDLDGLLF
                     TGSANTGYQLHRQLSGQPEKILALEMGGNNPLIIDEAADIDAAVHLTIQSAFVTAGQR
                     CTCARRLLLKSGAQGDAFLARLVAVSQRLTPGTWDDEPQPFIGGLISEQAAQQVVTAW
                     HELEAMGGRTLLAPRLLQAGTSLLTPGIIEMTGVTGVPDEEVFGPLLRVWRYDNFDEA
                     IRMANNTRFGLSCGLVSPEREKFDQLLLEARAGIVNWNKPLTGAASTAPFGGIGASGN
                     HRPSAWYAADYCAWPMASLESDSLTLPATLNPGLDFSDEVVR"
     gene            2129..>3063
                     /gene="astB"
                     /locus_tag="PN977_005207"
     CDS             2129..>3063
                     /gene="astB"
                     /locus_tag="PN977_005207"
                     /EC_number="3.5.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-succinylarginine dihydrolase"
                     /protein_id="HDI6045115.1"
                     /translation="MNAWEVNFDGLVGLTHHYAGLSFGNEASTRHRFQISNPRLAAKQ
                     GLLKMKNLADAGFPQAVIPPHERPFIPVLRQLGFSGSDEQVLEKVARQAPHWLSSVSS
                     ASPMWVANAATIAPSADTLDGKVHLTIANLNNKFHRSLEAPVTESLLKAIFDDEEKFS
                     VHSALPQVALLGDEGAANHNRLGGHYGEPGMQLFVYGREEGNDTRPSRYPARQTREAS
                     EAVARLNQVNPQQVIFAQQNPDVIDQGVFHNDVIAVSNRQVLFCHQQAFARQVQLLAN
                     LRARVNGFMAIEVPATQVSVSDAVSTYLFNSQLLSR"
BASE COUNT          636 a          811 c          931 g          685 t
ORIGIN      
        1 ctcgacccgg actggcgcaa agagggcaac ggctatttgc tgtcgaaatc gcgctttatg
       61 tttatggcgg cttttcgcga caagtttaat gacaaagtgg ttgctgaaat gcgcggggtg
      121 attgacgaac atggctattc accgttctgg caaagcctcg gtaagcgctt cttttcgatg
      181 gattttagcc gcgccgattt tctctgcggc accgggcaaa aggcatttat tgcagaactg
      241 atgccgaaac atccgatcta tacccacttt ttatctcagg aagcccagga cgtcatcggt
      301 caggtacatc cgcaaaccgc gcctgcccgt gcggtactgg agaaagaagg ttttcgctac
      361 cgtaattata tcgacatctt cgacggtggg ccgacgcttg agtgtgacat cgaccgcgtg
      421 cgcgccatcc gtaaaagtcg gctggtggaa gtggcagaag ggcagcctgc gcagggcgat
      481 tttccggcct gcctggtcgc caatgaaaat tatcaccatt tccgcgtggt gctggtgcgt
      541 accgatccgg caaccgagcg tttgatttta accgccgcac aactggatgc cctcaaatgc
      601 cacgcagggg atcgcgttcg tctggtgcgc ctgtgcgcag aggagaaaac agcatgactt
      661 tatggattaa cggtgactgg ataacgggcc agggcgcatc gcgtgtgaag cgtaatccgg
      721 tatcgggcga ggtgttatgg cagggcaatg atgccgatgc cgctcaggtc gggctggctt
      781 gtcgggctgc ccgtgcggcg tttccgcgtt gggcgcggct ctcctttggc gatcgtcagg
      841 taagagttga acgctttgcc gcactgctgg aaagcaataa agccgaatta accgcgatta
      901 ttgccagaga aacgggtaag ccgcgctggg aagcggcaac tgaagtgacg gcgatgatca
      961 ataaaatcgc gatatcaatt aaggcgtatc acgttcgtac cggcgagcag cgtagcgaaa
     1021 tgccggacgg cgcggcaagc ctgcgccatc gtccacacgg cgtgctggcg gtgtttgggc
     1081 cgtataattt ccccggtcat ttaccgaacg gacatatcgt tccggcattg ctggcaggta
     1141 acaccattat ctttaaaccc agcgaactga caccgtggag tggcgaagcg gtaatgcgtt
     1201 tatggcagca ggctggcttg ccgccgggcg tgctgaacct ggtgcagggc gggcgcgaaa
     1261 ccggtcaggc gctgagtgca cttgaggatc tcgacggctt gctgtttacc ggtagcgcca
     1321 ataccggcta ccagttgcat cgccagctct ccggtcagcc ggagaaaatt ctcgcccttg
     1381 agatgggcgg taataatccg ctaattatcg atgaggcggc ggatatcgat gccgctgtcc
     1441 atctgaccat tcagtcggcg tttgtcacag ccgggcaacg ctgcacctgc gctcgccgtt
     1501 tattgctgaa aagcggggcg cagggcgatg catttcttgc tcgcctggtt gccgtcagcc
     1561 agcggttaac gccgggcact tgggatgacg aaccgcagcc atttattggt gggctgattt
     1621 ctgagcaggc cgcacagcag gtggttactg cctggcatga actggaggcg atgggcggac
     1681 ggaccctgct tgcgccgcgc ttattacaag cagggacatc gttgctgacg ccggggatca
     1741 ttgaaatgac aggcgttact ggcgtgccgg atgaagaagt gtttgggccg ctattgcgcg
     1801 tctggcgtta tgacaatttc gatgaagcga ttcgaatggc gaataacact cgcttcggcc
     1861 tctcttgcgg tctggtttcc cccgagcggg aaaaattcga tcaactgttg ctggaggcac
     1921 gggcggggat tgttaactgg aacaaaccgc ttaccggtgc tgccagtacc gcgccattcg
     1981 gcggcattgg cgcttccggc aatcatcgcc ccagcgcctg gtatgccgca gattattgtg
     2041 cctggccgat ggcaagcctg gagtcggact cgttaacgtt gccagcaacg cttaaccccg
     2101 ggctggattt ttccgatgag gtggtgcgat gaacgcctgg gaagtcaatt tcgacgggct
     2161 ggtagggctg acgcatcatt acgcgggcct gtcgtttggc aatgaagcct ctacccgtca
     2221 ccgttttcag atctctaacc cgcggctggc ggcaaaacag ggcttactga aaatgaaaaa
     2281 ccttgccgat gcgggattcc cccaggcggt gatcccgccg cacgagcgcc cgtttattcc
     2341 ggtgctgcgt cagttgggtt tcagtggtag cgatgagcag gtactggaaa aggttgcacg
     2401 ccaggcaccg cactggcttt ccagcgtcag ctccgcttcg ccaatgtggg tagccaatgc
     2461 ggcaacgatc gcgccatctg ccgatacgct ggatggcaaa gtgcatctca ccattgctaa
     2521 cctgaacaat aaatttcacc gttcgctgga agcccccgtc accgaatcgc tgttaaaagc
     2581 gatttttgac gacgaagaga aatttagcgt ccattcggcg ttgccgcagg tagccttgct
     2641 cggtgatgag ggggcggcaa accacaatcg tctcggcggt cattacggtg aaccgggtat
     2701 gcaacttttt gtctacgggc gagaagaggg caatgatacc cggccttccc gttatccggc
     2761 gcgacagact cgcgaagcca gcgaggcggt ggcaaggctg aatcaggtga atccccaaca
     2821 ggtgattttc gcccagcaaa acccggacgt tatcgaccag ggcgtttttc ataatgacgt
     2881 gattgccgtg agtaaccgcc aggtgctgtt ttgccatcaa caggcgttcg ctcgccaggt
     2941 gcagttactg gcaaacctgc gtgcgcgggt gaacggtttt atggcgatag aagttccggc
     3001 gactcaggtt tccgtgtcag atgcggtgtc tacatatctg tttaacagcc aactgctgag
     3061 ccg
//
LOCUS       DAMZFT010000280         3036 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.280, whole genome shotgun sequence.
ACCESSION   DAMZFT010000280 DAMZFT010000000
VERSION     DAMZFT010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3036)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3036)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3036
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.280"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            35..262
                     /locus_tag="PN977_005208"
     CDS             35..262
                     /locus_tag="PN977_005208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HDI6045116.1"
                     /translation="MRLIVVSIMVTLLSGCGSIISRTIPGQGHGNQYYPGVQWDVRDS
                     AWRYVTILDLPFSLVFDTLLLPIDIHHGPYE"
     gene            complement(263..637)
                     /locus_tag="PN977_005209"
     CDS             complement(263..637)
                     /locus_tag="PN977_005209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309456.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secY/secA suppressor protein"
                     /protein_id="HDI6045117.1"
                     /translation="MTMYATLEEAIDAAREEFLADNPGIDAENANVQQFNAQKYVLQD
                     GDIMWQVEFFADEGEEGECLPMLSGEAAQSVFDGDYDEIEIRQEWQEENTLHEWDEGE
                     FQLEPPLDTEEGRAAADEWDER"
     gene            complement(720..1946)
                     /gene="mdtG"
                     /locus_tag="PN977_005210"
     CDS             complement(720..1946)
                     /gene="mdtG"
                     /locus_tag="PN977_005210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtG"
                     /protein_id="HDI6045118.1"
                     /translation="MSPCENDTPINWKRNLIVAWLGCFLTGAAFSLVMPFLPLYVEQL
                     GVTGHSALNMWSGIVFSITFLFSAIASPFWGGLADRKGRKLMLLRSALGMGIVMVLMG
                     LAQNIWQFLILRALLGLLGGFVPNANALIATQVPRNKSGWALGTLSTGGVSGALLGPM
                     AGGLLADSYGLRPVFFITASVLILCFFVTLFCIREKFQPVSKKEMLHMREVVTSLKNP
                     KLILSLFVTTLIIQVATGSIAPILTLYVRELAGNVSNVAFISGMIASVPGVAALLSAP
                     RLGKLGDRIGPEKILITALIFSVLLLIPMSYVQTPLQLGILRFLLGAADGALLPAVQT
                     LLVYNSSNQIAGRIFSYNQSFRDIGNVTGPLMGAAISANYGFRAVFLVTAGVVLFNAV
                     YSWNSLRRRRIPQVSN"
     gene            complement(2118..>3036)
                     /gene="lpxL"
                     /locus_tag="PN977_005211"
     CDS             complement(2118..>3036)
                     /gene="lpxL"
                     /locus_tag="PN977_005211"
                     /EC_number="2.3.1.241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) lauroyltransferase"
                     /protein_id="HDI6045119.1"
                     /translation="TNLPKFSTALLHPRYWLTWLGIGVLWLVVQLPYPVIYRLGCGLG
                     KLALRFMKRRAKIVHRNLELCFPEMSEQERRKMVVKNFESVGMGLMETGMAWFWSDRR
                     IARWTEVIGMEHIRDVQAQKRGILLVGIHFLTLELGARQFGMQEPGIGVYRPNDNPLI
                     DWLQTWGRLRSNKSMLDRKDLKGMIKALKKGEVVWYAPDHDYGPRSSVFVPLFAVEQA
                     ATTTGTWMLARMSGACLVPFVPCRKPDGKGYQLIILPPECSPPLDDAETTAAWMNKVV
                     EKCIMMAPEQYMWLHRRFKTRPEGVPSRY"
BASE COUNT          750 a          785 c          779 g          722 t
ORIGIN      
        1 ttatgccggt atttttttac ggaagaggta tcaaatgcgt ttaattgtgg tgagcatcat
       61 ggtgaccttg ctgagtggct gtggcagcat tattagtcgc actataccgg ggcaggggca
      121 tggcaaccaa tattatcccg gtgtgcaatg ggatgtgcgt gactccgcct ggcgttatgt
      181 cacgatcctt gatctgccat tctccctggt ttttgatact ttactgctgc cgatcgacat
      241 tcatcatggc ccgtatgagt aattaacgtt catcccactc atcagctgct gcgcgtcctt
      301 cctcggtatc cagcggtggc tcaagctgaa attccccctc gtcccattca tgtaatgtat
      361 tctcttcctg ccactcctgg cggatctcta tctcatcata gtcgccgtca aagacacttt
      421 gcgcagcttc accgctaagc atgggtaaac attcaccttc ttccccttcg tcggcaaaaa
      481 actcaacttg ccacatgatg tcgccgtcct gcaaaacgta tttttgggca ttgaactgtt
      541 gcacattcgc gttttcggcg tcgatgccgg ggttgtctgc aagaaattct tcgcgtgcag
      601 cgtcaatggc ttcttcaagc gttgcgtaca tggtcatcac gattctccct ttgagttgat
      661 gaggtttcag ggaaaaggat agctgattct ccgcttttgc aagtatgaaa ggcgaaaaat
      721 cagttcgata cctggggtat tcgacgacga cgtagactgt tccatgaata gactgcgttg
      781 aataacacta cgccagcggt gacgagaaat accgctctga aaccgtagtt cgctgaaatc
      841 gcggctccca tcaatggtcc ggtaacgttt ccaatatcac gaaacgactg gttatagctg
      901 aagatacgcc cggcgatttg gttgctcgag ttgtaaacca acagtgtctg tacggcgggg
      961 agtagtgcac catcggcggc accgagcaaa aaacgtaaaa tcccaagttg taatggcgtc
     1021 tgaacgtatg acattgggat caacagcagt acagaaaaga tcagcgccgt aatcaggatc
     1081 ttttcggggc cgattcgatc gccaagtttg ccgagtcgtg gtgcactcag cagagccgcc
     1141 acgcctggca ccgaggcgat catgccactg ataaaagcga cgttactgac gttacccgcc
     1201 agttcgcgga catacagcgt cagaatgggg gcaattgagc ccgtcgccac ctggatgatt
     1261 aacgtggtga caaacaggct gagtatcagt ttcgggtttt taagtgatgt caccacttcc
     1321 cgcatatgca gcatctcttt tttgctgacc ggctggaatt tttctctgat gcaaaacagg
     1381 gtgacgaaaa agcagagtat gagcacactg gcggtaataa agaataccgg acgtaagccg
     1441 tagctatcag cgagcaaacc gccagccatt gggccgagca acgcgccgct aacgccgcct
     1501 gtggagagcg tacccagcgc ccagccgctt ttattacgcg gtacttgtgt ggcgataaga
     1561 gcattagcgt tggggacaaa tccgccaagt aaaccaagaa gcgcccgcag gatcaaaaac
     1621 tgccagatat tttgtgccag tcccatcaac accatcacga tgcccatgcc gagagcagag
     1681 cgtaatagca tgagttttcg gcctttacgg tcggcgagtc caccccaaaa cggtgaggcg
     1741 atggccgaaa ataaaaatgt aatgctgaag acaataccgg accacatatt cagggcggag
     1801 tgaccggtaa cgccaagctg ctcaacgtag aggggtaaga agggcattac cagactgaag
     1861 gcggcaccgg taagaaaaca gcctagccag gcgacgatca ggtttcgttt ccagtttata
     1921 ggggtgtcat tttcacaggg tgacatagca atccgctgtt ggtgcgccag gcgcggtgaa
     1981 cataagaaga aaagataagc acactaatta tgcgcccgac ttccaggggg cgcaatccag
     2041 agagctttta tcgctaaatc agggggattt gctgtggtaa tgccggatgc cattctgaag
     2101 catccagcat ggaagattta atagcgtgaa ggaacgcctt ccgggcgtgt tttaaagcga
     2161 cggtgtaacc acatatactg ctctggtgcc atcatgatgc atttttcgac cactttgttc
     2221 atccacgcgg cggtggtttc ggcatcatcc agcggcggag aacactctgg cggcagtata
     2281 atcaactgat accctttgcc atctggctta cggcatggaa cgaagggcac cagacatgcg
     2341 ccggacatcc gtgccagcat ccaggttccg gtcgtggtcg cagcctgctc aacggcaaac
     2401 aacgggacga aaacgcttga gcgcgggccg taatcatgat ccggtgcgta ccagaccact
     2461 tcgccttttt tcagggcttt aatcatgcct tttaaatctt tgcggtcgag catcgattta
     2521 tttgagcgta aacggcccca ggtttgtagc cagtcaatca gtggattatc gttcgggcgg
     2581 taaacgccaa tccccggttc ctgcatacca aactgccgcg caccaagctc cagtgtcaga
     2641 aaatggatgc caactaacag gatgccgcgt ttttgcgcct gcacgtcacg aatgtgttcc
     2701 atgccgatca cttccgtcca gcgggcgatt ctgcggtccg accagaacca cgccatgcca
     2761 gtttccatca gtcccatgcc aacggattcg aaattcttca ccaccatttt acggcgttct
     2821 tgttcgctca tttccgggaa gcacagttcc aggttgcgat gcacaatttt tgcgcgtcgt
     2881 ttcataaaac gtaacgcaag ttttcctaat ccacaaccga ggcggtagat aaccgggtag
     2941 ggcaattgca cgactaacca aagtacgcca atacccaacc aggttaacca ataacgcgga
     3001 tgaagcagtg cggtggagaa cttgggtaga ttcgtc
//
LOCUS       DAMZFT010000281         3032 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.281, whole genome shotgun sequence.
ACCESSION   DAMZFT010000281 DAMZFT010000000
VERSION     DAMZFT010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3032)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3032)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3032
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.281"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            977..1201
                     /locus_tag="PN977_005212"
     CDS             977..1201
                     /locus_tag="PN977_005212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001470144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6045120.1"
                     /translation="MSENTGKSHENKMVIIDYGEYDEYLRNAWFRGFREGYDMAMKFG
                     LGCQQCYETGEKIACARAEECRKKIKVIKH"
     gene            complement(1330..1854)
                     /locus_tag="PN977_005213"
     CDS             complement(1330..1854)
                     /locus_tag="PN977_005213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000823671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase family protein"
                     /protein_id="HDI6045121.1"
                     /translation="MKRKLIAFVILATSSCGYAATKDVTMNLVDSDGKERNIGKITIQ
                     KTPYGLLFTPDLHSLPAGIHGFHVHENASCAPALKDGKTVAALSAGGHFDPEKTGKHL
                     GPWSPDGHLGDLPALYVTHDGKATYPVLAPRLSELKELDGRSLMLHAGGDNHDDHPEP
                     LGGGGARIACGIIP"
     gene            complement(2203..2473)
                     /locus_tag="PN977_005214"
                     /pseudo
     CDS             complement(2203..2473)
                     /locus_tag="PN977_005214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001167424.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2775..>3032
                     /locus_tag="PN977_005215"
     CDS             2775..>3032
                     /locus_tag="PN977_005215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_025243830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HDI6045122.1"
                     /translation="MDDTQLQALANELAKNLKTPEDLSQFDRLLKKISVEAALNAELT
                     HHLGHEKNQTKSGTNFRNGYSTKTVTTTDGPMELRTPPDRDG"
BASE COUNT          891 a          642 c          672 g          827 t
ORIGIN      
        1 actggattcc cggtatgagt aacttttatt aacgataagc caggctattt gtgcattggt
       61 gttgaggtga gaatagcacc agtcaggtga gcagattata atgttacgag acttaagcac
      121 ctctgtagct cccatggtct gatagtttct cccgtatacc gctcatgcaa ctcccgcatt
      181 aaaatatact gttccttccg gataattacg ttaaaatata cacctgcgtg catctgtgtc
      241 agactccgtt agataccagc gtacattgta aacatgccgg atgcacgcaa tacaataatc
      301 ggtcttttcg gagttatagt caccaggctg gcatcccgta aatatgtatc aggtagcaat
      361 tcaggaagcg aacaaaagcg cctggagaag gttatctctg ataataattt acatgaaatg
      421 tctcctttat tcttcgcgaa taaggccttc agtgttctcc ccttttcagc aattcttacc
      481 gtagtaatca ctcctgcctg tgtataacac gagaattgcc agagtttaac gtttcggtat
      541 cgttatcctg catgatgttg aacagaaccg ggcgtgaatt ctgcgccaga attttctttc
      601 gaggaacatt ccgggttatc tgtagtacag gaggagattt tctcctattt caaataaaat
      661 tcaatgacag gtgaatattt aaatataaat gcatcgcttc aacagagaaa tcggaattac
      721 gcccggaacc aggcctgaat gaataatgga ggaggtaaaa taatattatt gatgaaaata
      781 tcaaattaag tcccccttct ccttccccag cttaaatata cttacttttt cactggataa
      841 tatgttattg aattacaaag ttgatcctga tatacatgat ttccggctct tgcatccagt
      901 gataacttta tatagtcctg caaaggcgaa cttcgtaaaa gtatactttt ctgttgtaag
      961 cattgaggta tcgaaaatga gtgagaatac cggaaaatcc catgaaaata aaatggttat
     1021 tattgactac ggggaatatg atgaatacct tcgtaacgcg tggttccgag gttttcggga
     1081 aggttatgat atggcgatga aatttggatt aggctgccag caatgttatg aaacaggtga
     1141 aaaaatagca tgcgcaagag ctgaagaatg ccgtaaaaag attaaggtaa taaaacactg
     1201 aaacgggctg tctgatattt ctgtgagcta aaaagacacc gtaatacagg tatctcacag
     1261 taactgaaag atatgcacat gccgccaggg aagattaagg gtaagggagg atatccctta
     1321 cctgattgat tatgggatga taccacaggc aattcttgca ccaccgccac ctagaggttc
     1381 aggatggtca tcatgattat cacctcctgc gtggagcatt agcgaacggc catcgagctc
     1441 ctttaattca gatagtcttg gcgcaagcac cgggtaggtt gcttttccgt catgagtgac
     1501 ataaagcgca gggagatcac ctaggtggcc atcaggagac catggaccga gatgcttccc
     1561 tgttttttcc gggtcaaagt gaccacctgc agataaagca gcgacagttt ttccatcttt
     1621 cagggcagga gcacaacttg cgttttcatg tacatgaaaa ccatgaatgc cggcgggtaa
     1681 ggagtgaaga tcaggggtga acagcagccc gtagggtgtc ttctggatgg ttattttacc
     1741 aatatttcgc tcttttccgt cactatcaac aagattcatt gttacatctt tggtggctgc
     1801 atatccacag gatgatgtag ccagaatcac aaaggcaata agtttacgtt tcataaaccc
     1861 tctgttaaca ggttaaagat ttactcaata actgttgcga aataacagtc agccagatga
     1921 tagcttatga tagttattga atgtgactat cagtgtaatt aattatgcaa attagaagga
     1981 atgttatgtg tgaagcgccc ctgtatcccg ctcatactgg ctaacccaga cacgcagagt
     2041 ctgtggcgta cagccagcat ttgaggcaat ggaacaaatt accccaattg tgaatcatac
     2101 tcatcctgtt tacctctttc tcagggagtt tagtctccag gattcccgga gcagttcaaa
     2161 gtgccctgtg ctgcttaaag tgccgctgca tgccaccaca tggctgagca gcaacaggta
     2221 ttgttgttcc ttcatgttgt tcacgcaacc agcgtaatac caccggcgag gatggactgt
     2281 cagtgtgatt acgttcagta atacgttcat aaaaggaagt tcagaatgca tgaatctgtt
     2341 gtccgttgat gtttccttct gtcgaattgt gtgtgaagtg gacaatcaga aaatgcggcc
     2401 agtgatccac tgcctgttga ataaaacgcc ggatgtctcc cggctctgca agtaatgctt
     2461 cagtttcctg cactatttta tccctgttga attttgtcaa accggtgcag acacgcagga
     2521 aagtatgcca gcactggtgt aatgccagta acatacaata agaatgaaat gacgaatcag
     2581 tcaggaaagg gtgcccccat tggcaaccca ttgactgagc agaggataca atcaggaagg
     2641 ttaacgagag tatctccgac atcaggggca ggattgattt ctacaatacc agcgccggca
     2701 ttgagcctgt acatagattt atgtaattgc ctgattttga tctgttcaat tcaacatcaa
     2761 atgaaagcta aattatggac gacacacaat tgcaggctct ggctaacgaa ctggccaaaa
     2821 atctcaaaac ccctgaagat ctcagtcagt tcgatcgcct gctcaagaaa ataagtgttg
     2881 aggcagctct caacgctgaa ctgactcacc atctgggaca tgaaaaaaat cagaccaaat
     2941 cgggaaccaa cttccgtaat ggctattcca cgaaaaccgt caccactacc gacgggccga
     3001 tggaattacg tactccacct gatcgtgatg gt
//
LOCUS       DAMZFT010000282         2981 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.283, whole genome shotgun sequence.
ACCESSION   DAMZFT010000282 DAMZFT010000000
VERSION     DAMZFT010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2981)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2981)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2981
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.283"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..439
                     /locus_tag="PN977_005216"
     CDS             <1..439
                     /locus_tag="PN977_005216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000095522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDI6045123.1"
                     /translation="TISQQVAWLLGLSAIAALIAQFVVLRPQRLTPVALLLSAGVLMS
                     SGLAIMLAEQLWLFYLGCAVLSFGAALATPAYQLLLNDKLADGAGAGWLACSHTLGYG
                     LCALLVPLVSKTGVAIALIVMALFAAVLFSMVTVFIWRCCKSK"
     gene            627..1883
                     /locus_tag="PN977_005217"
                     /pseudo
     CDS             627..1883
                     /locus_tag="PN977_005217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001368704.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            1933..2527
                     /locus_tag="PN977_005218"
                     /pseudo
     CDS             1933..2527
                     /locus_tag="PN977_005218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001347735.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            2724..>2981
                     /locus_tag="PN977_005219"
     CDS             2724..>2981
                     /locus_tag="PN977_005219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDI6045124.1"
                     /translation="MDEKKLKVLAAELAKGLKTEADLNQFSRMLTKLTVETALNAELT
                     DHLGHEKNAPKSGSNTRNGYSSKTVLCDDGEIELNTPRDREN"
BASE COUNT          788 a          704 c          740 g          749 t
ORIGIN      
        1 cactattagc caacaggtag cgtggttgtt ggggctgtcc gcaatagctg cgcttatcgc
       61 gcagttcgtg gtactccgtc cacagcgcct gactccagtg gctctgctcc tgagtgccgg
      121 ggtgttgatg agtagtggtc tggctatcat gctcgctgaa cagctatggt tgttttacct
      181 aggctgtgca gtgctgtcat ttggagctgc tctggcaacc cccgcttatc aacttttact
      241 gaatgataag ctggccgacg gcgcaggcgc gggctggctc gcttgcagtc acacacttgg
      301 ctatgggctt tgcgcgttgt tggtaccatt ggtgtcgaaa acaggtgtcg caatagcact
      361 gattgtgatg gcattatttg ccgctgtatt atttagcatg gtgactgtat ttatctggcg
      421 ctgctgcaaa agcaagtaat gatatgagct caatctactt tcgacgcgac tggttcacca
      481 cttttccata aactctgtat gatttcggct ttgagagggt cagcatacat cttttgcatt
      541 tataacgaac ggttcgacga aagagggctt ccacaacttg agtgaaagaa aatgtatgag
      601 gcttaccagt gaagccgttt taagttatgg gtaacttaat acactcactt aagagatttc
      661 tacggttact ttgctgatgg caatacggtc aagaagttat tagctctgac ggtatataac
      721 cctccatctc cccggaaaat gcaaaacagc actatgtgtt gaatacctta agcacagcta
      781 ctcagttcat agatgccatc gcctccagat acagcactta ccctgctcag cccagacgca
      841 tcttactctg gtgctgccat aacccttgcc tgttaacgaa aaattttgcc gagtctcagc
      901 ctcccttcac agttcacatc agcagtattc acaggcgtta caaaatttac aacgtcaacc
      961 gggattaata gccggaggtg aggccgggaa acaggcagca acggcagtgg ggctacgttg
     1021 cagtgctgat actcttcttc gcagggttat taataccccg gagacgaaac agtcgggcgc
     1081 gcctcatgtc ggtattgatg agtgggcgtg gcatcggggc caccattacg gcacgttaat
     1141 cgtcaatctt gatactcacc gtcccctcgt cctgcttccc ggttgcgatc agagtacgct
     1201 ggcgacctgg ttcagaaaat atccggaaat acaggttgtc tcacgtgacc gtggcggagt
     1261 ctatgcgaca gcagcacgtg aaggtgcacc tcaggccaga cagatagctg atcgatggca
     1321 cctgctaaaa aatattggcg atgcgcctga acgaatgatg tacagacaga tgcctctgat
     1381 acgtcttgtt gccagtgggt tgtcaccaaa gaaatcacct aagccagtac tgtctgtgcc
     1441 tgtagcatcg ctccgtcgtc cggaacgcct taaacagcaa acccgccaaa aacggcatca
     1501 gcgttggaca gaggttatgg cactacataa caagggatgt gggttcaggg gaaatatccc
     1561 gtattacagg cttatcacgt gtgacagtca gtctctgggt gtgttcagga acattccctg
     1621 aaatgtcaac ccggcctcca gagcgaggac ttctggcccc atggagggag tggttaaaag
     1681 agcaacgaga aagtggtaac ttcggcgcgt ggctgcaagg atggcagctg atgctgcggc
     1741 tatatgtgag gcaatcagca gtcgctggaa taatgggggt tgtcgaaagt catgtaaatc
     1801 gactaaaaat gctgaaaggc cagatgtatg gtcgagccgg atttgaacta ctcaggcaga
     1861 gggtcatgag tccactggca tgaaaatctc catccaggtt gcgtaagagc cacttttcat
     1921 ccgtcgttga catttgtatg ccgtcactgt ggcacttctc ggcccacatt gtgtaaatgg
     1981 tggcgcagat atctggctca gggcatcgct ggtctggaaa gctacagccg tcgctccaag
     2041 cgttcaccat caacgaaaac cagcacttgc gaagtcgctc tgattctgga agttacgttc
     2101 tcagcgaaat ctgggtgcaa gacgtattca aagcgagtta aaacgacttc attcaatctc
     2161 actggcaata gtgcgtatta ccgtgaaggg gatcggtgag taacggcgtt tttcaaggta
     2221 gttgtcgcct gatgtaataa aggttggacg ttcccaaaag acagacaaag ctgagtttta
     2281 cgctaccgta gatctttctg ctgatgacct gaagggattg ctagcagaat ggtagcatta
     2341 ctataacagg gaacgtcctc atagcgctca caacggtaaa actccgatgg aacgctactt
     2401 tgaacttgct gagaaaacac catactccga tgccgttcat gcaaactatc agcccaatga
     2461 agagcatata caggaacaga attacaagcc tgaacttgaa ctgagaaaat tgaaacgatg
     2521 tctatgaatc acacatttta ttacaaggca acaactacct tcaagctcta tgttgagcct
     2581 gtttaccatt ctgtgtaata tgcattttct ttaaagtgac cgtcaggacg gtcactgatt
     2641 acccattctg aagagactgt aattaaaatt gtgtaattgc ctgtttttga tatgttcact
     2701 ccaacaacgg agacaggcaa acgatggacg aaaagaaact taaagtcctt gcggctgaac
     2761 tggccaaagg ccttaaaacc gaagccgacc tcaaccagtt ttcacgcatg ttgacaaagc
     2821 ttaccgtcga aacggcgctc aatgcagaac tgactgacca tctaggtcat gagaaaaatg
     2881 ctcctaaatc aggctctaat acccgtaatg gctactcgtc aaaaacggta ttgtgcgatg
     2941 atggcgagat cgaacttaac acgccgcgtg accgtgaaaa t
//
LOCUS       DAMZFT010000283         2928 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.284, whole genome shotgun sequence.
ACCESSION   DAMZFT010000283 DAMZFT010000000
VERSION     DAMZFT010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2928)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2928)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2928
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.284"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..2364
                     /locus_tag="PN977_005220"
     CDS             <1..2364
                     /locus_tag="PN977_005220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HDI6045125.1"
                     /translation="EWSNAATFEKLEVDAERGVITEEWRAHQDAKWRTSQARRPFLLA
                     NTRNLDREPIGLMDTVATVTPAQLRQFYQRWYQPNNMTFIVVGDIDSKEALALIKDNL
                     SKLPANKAAENRVWPTKAENHLRFNIINDKENRVNGIALYYRLPMVQVSDEQSFIEQA
                     EWSMLVQLFNQRLQERIQSGELKTISGGTARSVKIAPDYQSLFFRVNAQDDNMQDAAN
                     ALMAELATIDQHGFSAEELDDVKSTRLTWLKNAVDQQAERDLRMLTSRLASSSLNNTP
                     FLSPEETYQLSKRLWQQITVQSLAEKWQQLRKNQDAFWEQMVNNEVAAKKALSPAAIL
                     ALEKEYANKKLAAYIFPGRNLSLTVDADPQAEISSKETLAENLTSLTLSNGARVILAK
                     SAGEEQKLQITAVSNKGDLSFPAQQKSLIALANKAVSGSGVGELSSSSLKRWSAENSV
                     TMSSKVSGMNTLLSVSARTNNPEPGFQLINQRITHSTINDNIWASLQNAQIQALKTLD
                     QRPAEKFAQQMYETRYADDRTKLLQENQIAQFTAADALAADRQLFSSPADITFVIVGN
                     VAEDKLVALITRYLGSIKHSDSPLAAGKPLTRATDNASVTVKEQNEPVAQVSQWKRYD
                     SRTPVNLETRMALDAFNVALAKDLRVNIREQASGAYSVSSRLSVDPQAKDISHLLAFT
                     CQPERHDELLTLANEVMVKRLAKGISEQELNEYQQNVQRSLDIQQRSVQQLANTIVNS
                     LIQYDDPAAWTEQEQLLKQMTVENVNTAVKQYLSHPVNTYTGVLLPK"
     gene            2726..>2928
                     /locus_tag="PN977_005221"
     CDS             2726..>2928
                     /locus_tag="PN977_005221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HDI6045126.1"
                     /translation="MDKKQVTDLRSELLDSRFGAKSISTIAESKRFPLHEMRDDVAFQ
                     IINDELYLDGNARQNLATFCQTW"
BASE COUNT          885 a          661 c          656 g          726 t
ORIGIN      
        1 gaatggagta atgccgcaac ctttgaaaaa ctcgaagtag acgctgaacg tggcgtaatt
       61 actgaggaat ggcgtgccca tcaggatgca aaatggcgca cctctcaggc gcgccgccct
      121 ttcctgctgg caaatacccg taatttagac cgtgaaccta tcggcctgat ggataccgtc
      181 gccacggtca caccggcaca attgcgccaa ttttatcaac gctggtatca accaaataat
      241 atgaccttta ttgtggtcgg cgatatcgac agtaaagaag cgctggcgct gataaaggat
      301 aatttaagta agcttccggc taacaaagca gctgaaaatc gcgtctggcc gacaaaagcc
      361 gaaaaccacc tgcgctttaa tatcatcaat gataaagaaa accgggtgaa cggcatcgca
      421 ctctattatc gcctgccaat ggtgcaagtg agcgatgagc aaagctttat cgaacaagct
      481 gaatggagca tgttagttca gctgttcaac caacgtctgc aagaacgcat acagtcgggc
      541 gagttgaaga ctatttctgg cggcactgcg cgcagcgtca aaattgcacc cgattatcag
      601 tcgctgtttt tccgtgtaaa tgcacaagac gataatatgc aggatgctgc gaatgcatta
      661 atggcagagt tggcaaccat tgatcagcat ggcttttctg ctgaagaact cgatgatgtt
      721 aaatctaccc gccttacctg gctgaaaaat gcggttgatc agcaagctga gcgtgattta
      781 cgtatgctga ccagtcgcct ggcatccagc tcattaaata atacgccgtt cttgtcgccg
      841 gaagagacat atcaactttc gaaacgtctg tggcagcaaa ttaccgtgca aagtctggcg
      901 gaaaaatggc agcagttaag aaagaaccag gacgcatttt gggagcaaat ggtaaacaat
      961 gaggttgccg ccaaaaaagc attgtctcct gcggctatcc tggcgctgga aaaagagtac
     1021 gccaacaaaa agctggcggc ttacatcttc ccaggcagaa atttatcgtt aacagtagac
     1081 gctgacccac aggcggaaat tagcagcaaa gaaacgctgg ctgagaatct gacatcatta
     1141 acactttcca atggtgccag ggttattctg gcaaaatccg cgggtgaaga gcaaaagcta
     1201 caaattactg ccgtatctaa taaaggcgat ttaagtttcc ctgcgcagca aaaatcactt
     1261 atcgcgctgg caaataaagc agtaagcgga agcggcgttg gtgaactctc ctcttccagc
     1321 ctgaaacgct ggagtgcgga aaactcggta accatgagca gtaaagtcag tggcatgaat
     1381 acattgctct ctgttagcgc gcggactaat aaccctgaac ctggtttcca gttgattaac
     1441 cagcgaatca cccacagcac gattaacgat aatatttggg catcgctaca aaatgctcaa
     1501 attcaggcgt tgaaaacgct cgaccagcgt ccagcggaga aattcgccca gcagatgtat
     1561 gagacgcgct atgctgatga ccgcacgaaa ttactgcaag aaaatcagat tgcacagttt
     1621 actgccgcag atgcgctggc tgccgatcgc cagttgtttt catctccagc ggatatcacc
     1681 tttgtcattg tcggtaatgt cgcagaagac aaactcgtgg cgttaattac gcgttactta
     1741 ggatcaatca aacactctga ttcgccatta gccgcaggta aaccattaac tcgcgcgacg
     1801 gacaacgcat cggttactgt aaaagaacaa aatgaacctg tggcacaggt ttcacagtgg
     1861 aagcgttatg attcccggac acctgtaaat ctggagacgc gtatggcgct cgatgctttt
     1921 aacgtcgcac tggcaaaaga tctacgcgtt aatattcgtg aacaggcatc tggagcatac
     1981 agcgtttctt ctcgcctctc ggttgatcct caggccaaag atatcagtca tttgctggct
     2041 tttacttgtc aaccagaacg acatgatgaa ctgttaacgt tagcgaatga agtgatggtt
     2101 aagcgcctgg ctaaagggat cagtgagcaa gaactgaatg aataccagca aaacgttcag
     2161 cgcagcctcg atatccaaca gcgtagcgtt caacaattag cgaacaccat tgtaaatagt
     2221 cttattcaat atgacgatcc tgcagcatgg actgagcagg agcaattatt gaaacaaatg
     2281 acggtagaga atgttaacac tgccgttaaa caatatcttt ctcatccggt gaatacctat
     2341 accggagtat tattgccaaa ataataacag ccccgtcaac acctcgttgg cggggatttt
     2401 agcaatattc gccattttta tgtaataatt ttataaatgc gttcaaaata atagccgagt
     2461 gctaatagtg atattttaag gtctgatttt tacgtgataa ttcaggaggc gtagaaagtg
     2521 cataaaaata acagcataaa acaccttgcc accacccaag aattcaataa tatattgatt
     2581 ttcaatgata aaatataatt cgcataatat ctcgcgataa ataacattag gattttgtta
     2641 tttaaacacg agtcctttgc acttgcttac tttatcgata aatcctactt ttttaatgcg
     2701 atccaatcat tttaaggagt ttaaaatgga taagaagcaa gtaacggatt taaggtcgga
     2761 actactcgat tcacgttttg gtgcaaagtc tatttccact atcgcagaat caaaacgttt
     2821 tccgctgcac gaaatgcgcg acgatgtcgc atttcagatt atcaatgacg aattatatct
     2881 tgatggcaac gctcgtcaga acctggccac tttctgccag acctggga
//
LOCUS       DAMZFT010000284         2792 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.285, whole genome shotgun sequence.
ACCESSION   DAMZFT010000284 DAMZFT010000000
VERSION     DAMZFT010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2792)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2792)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2792
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.285"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(<1..1224)
                     /locus_tag="PN977_005222"
     CDS             complement(<1..1224)
                     /locus_tag="PN977_005222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA repeat-containing protein"
                     /protein_id="HDI6045127.1"
                     /translation="MGSDAKNLMSDGNVQIVKTGEVIGATQLTEGELIVEAGGRAENT
                     VVTGAGWLKVATGGIAKCTQYGNNGTLSVSDGAIATDIVQSEGGAISLSTLATVNGRH
                     PEGEFSVDKGYACGLLLENGGNLRVLEGHRAEKIILDQEGGLLVNGTTSAVVVDEGGE
                     LLVYPGGEASNCEINQGGVFMLAGKANDTLLAGGTMNNLGGEDSDTIVENGAIYRLGT
                     DGLQLYSSGKTQNLSVNVGGRAEVHAGTLENAVIQGGTVILLSPTSADENFVVEEDRA
                     PVELTGSVALLDGASMIIGYGADLQQSTITVQQGGVLILDGSTIKGDSVTFSVGNINL
                     NGGKLWLITGAATHVQLKVKRLRGEGAICLQTSAKEISPDFINVKGEVTGDIHVEITD
                     ASRQTLCNALKLQPDE"
     gene            1532..1608
                     /locus_tag="PN977_005223"
     tRNA            1532..1608
                     /locus_tag="PN977_005223"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1566..1568,aa:Val,seq:gac)
     gene            1613..1689
                     /locus_tag="PN977_005224"
     tRNA            1613..1689
                     /locus_tag="PN977_005224"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1647..1649,aa:Val,seq:gac)
     gene            1798..2103
                     /locus_tag="PN977_005225"
     CDS             1798..2103
                     /locus_tag="PN977_005225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monooxygenase"
                     /protein_id="HDI6045128.1"
                     /translation="MATLLQLHFSFNGPFGDAMAEQLKPLAESINQEPGFLWKVWTES
                     EKNHEAGGIYLFTDEKSALAYLEKHTARLKNLGVEEVVAKVFDVNEPLSQINQAKLA"
     gene            2229..>2792
                     /locus_tag="PN977_005226"
     CDS             2229..>2792
                     /locus_tag="PN977_005226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HDI6045129.1"
                     /translation="MKKIAIVGAGPTGIYTLFSLLQQQTPLSISIFEQADEAGVGMPY
                     SDEENSKLMLANIASIEIPPIYCTYLEWLQKQEASHLQRYGVKKETLHDRQFLPRILL
                     GEYFRDQFLRLVDQARQQKFAVAVYESCQVTDLQITNAGVMIATNQDLPSETFDLAVI
                     ATGHVWPDEEEATRTYFPSPWSGLMEAK"
BASE COUNT          717 a          730 c          591 g          754 t
ORIGIN      
        1 ttcgtctggc tgtagtttca gtgcgttaca cagagtttgc cgactggcat ctgttatctc
       61 aacgtgtata tcaccagtaa cttccccttt cacattgatg aagtcaggtg aaatttcttt
      121 cgcactggtt tgcaggcaaa tcgctccctc tccgcgcagg cgtttcactt taagttgcac
      181 atgcgttgcc gcaccagtga ttagccacag ttttccgcca ttgagattga tgttaccaac
      241 actgaaagtg acactgtcac cttttatcgt actgccgtcg agaatcaata caccgccctg
      301 ctgtacagta atcgttgatt gttgcagatc tgcgccatag ccaataatca ttgaagcgcc
      361 gtccagtaat gcaacactac cggtcagttc aaccggtgcg cgatcttcct ctacgacaaa
      421 attttcgtcc gcgctggtgg gtgacaacag gatcactgtc ccaccttgta ttaccgcatt
      481 ttccagcgta ccggcatgca cttcagcccg accacccaca ttaacggaca ggttttgcgt
      541 cttaccggaa ctgtagagct gaagaccatc cgtccccaga cgatagatgg ctccattctc
      601 aacaatagtg tcagagtctt caccaccgag attattcatg gtgccaccag caagcaacgt
      661 atcattggct ttccccgcca gcataaaaac gccgccctga ttaatctcac aattgctggc
      721 ttccccacct ggatacacca acaattcacc accttcatct accacgaccg ctgaggttgt
      781 cccattgacc aacaggccac cctcttgatc gagaataatt ttttccgcgc gatggccttc
      841 cagtacacgc aggttaccgc cattttccag caacaaaccg caggcataac ctttatcaac
      901 gctgaattca ccttcgggat ggcggccatt aaccgtagcg agcgtagaga gactaattgc
      961 gcctccctcg gactgaacaa tatctgtggc aatggcaccg tcgctgaccg atagcgtgcc
     1021 attgttgccg tactgtgtgc atttggcgat cccaccggtt gccactttca accagccagc
     1081 ccccgtgacc acggtatttt cggctcttcc gccagcctca acaattaact cgccttcagt
     1141 aagttgcgtc gcgccaatga cctcgccggt cttaacaatt tgcacgttcc cgtcgctcat
     1201 caagtttttc gcatcagatc ccatgattta ttcctttgct gcatctgtgt gcctttattg
     1261 ctacctaagt gtaaaggcta cgaaggattt atccacgaca gatttgagat ggtggcaaac
     1321 aactctgttt aaactctgat acacggatta ttgggttgta tcagatgtaa atgcgatcct
     1381 gaataaaaat cgcccttgca aatcaacaaa atatagccaa tttgcttaaa agctctgcaa
     1441 acggtcgaaa taacgcagaa aattacgttt tgcctcttgc caccttccca ctctgccgct
     1501 aatattcgtc cccgttgtca cctacaacgt tgcgttcata gctcagttgg ttagagcacc
     1561 accttgacat ggtgggggtc gttggttcga gtccaattga acgcaccatc ctgcgtccgt
     1621 agctcagttg gttagagcac caccttgaca tggtgggggt cggtggttcg agtccactcg
     1681 gacgcaccag attttcttaa tctggtcttc tccttttttc cctctgtttc ttctctgtat
     1741 ccaatacgtt aaaagattta cactgtcttc gtatgcgtta tcagtaggag aatcgctatg
     1801 gcaactttgt tacaacttca tttttctttc aatggcccat ttggcgacgc aatggctgag
     1861 cagcttaaac ctcttgctga gtcgattaat caggaacctg gttttctgtg gaaggtatgg
     1921 acagaaagtg aaaagaacca cgaagccggt gggatctacc tttttactga tgaaaaaagc
     1981 gcccttgcct atctggaaaa acatactgcc cgactgaaaa atctcggcgt tgaggaagtt
     2041 gtcgccaaag ttttcgatgt caatgagcca ctgagtcaaa tcaatcaggc aaaactcgcc
     2101 tgacagaatt taatcaaggg cggttagcgc ccttttcatc cctgtctgaa atttctcaaa
     2161 ttctaaaaat ctcaaccaaa cttatctgat aacactaaat tcgaaagaat gcgtacaggt
     2221 aagtaacaat gaaaaaaatt gctattgtgg gtgccgggcc tacggggatc tacaccttat
     2281 tctcgcttct acagcaacaa acgccacttt ctatttctat cttcgagcag gctgatgaag
     2341 ccggtgtcgg gatgccatac agtgatgagg aaaactcaaa actgatgctg gcaaatattg
     2401 ccagtattga aataccgccg atttattgta cgtatctcga atggctacaa aagcaagaag
     2461 ccagccatct ccaacgttat ggcgttaaaa aagaaacctt gcacgatcgt cagtttttac
     2521 cgcgaattct gctgggcgaa tatttccgcg atcaattttt acgattagta gaccaggcac
     2581 gacagcaaaa atttgcagtg gctgtttatg aatcatgcca ggttaccgat ctgcaaatta
     2641 caaatgctgg cgtcatgatc gctacaaatc aggatttacc cagcgagacg tttgatttag
     2701 cggtgattgc cacgggtcac gtctggcctg atgaagaaga agcaaccaga acgtattttc
     2761 ccagcccgtg gtcaggcctg atggaagcaa ag
//
LOCUS       DAMZFT010000285         2783 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.286, whole genome shotgun sequence.
ACCESSION   DAMZFT010000285 DAMZFT010000000
VERSION     DAMZFT010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2783)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2783)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2783
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.286"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(<1..284)
                     /locus_tag="PN977_005227"
     CDS             complement(<1..284)
                     /locus_tag="PN977_005227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001569361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
                     /protein_id="HDI6045130.1"
                     /translation="MSNVLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKM
                     LLSVCRSWAAWCKLNNRKCFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQL"
     gene            complement(291..650)
                     /locus_tag="PN977_005228"
     CDS             complement(291..650)
                     /locus_tag="PN977_005228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007374288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDI6045131.1"
                     /translation="MNIKPIRTEQDYQAALRAVEPMFDNEPEMNTPEGDFFEVMCLLI
                     EEYEKRHYPIEPPTPIEAIKFRMEQQGLTVKDLEPAIGKSNRVYEVLNGTRNLTLPMI
                     RRLHVQFGIPLESLVGV"
     gene            complement(654..956)
                     /locus_tag="PN977_005229"
     CDS             complement(654..956)
                     /locus_tag="PN977_005229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004142601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HigB family
                     toxin"
                     /protein_id="HDI6045132.1"
                     /translation="MKIISVKTLRDFWSVNPDAEQPLKAWVDEVSKATWKSPADIKAQ
                     YRSASILKNRRVVFNIKGNDYRLIVAIAYQRGWMFVKFIGTHKEYDAIDADTIELE"
     gene            1609..1818
                     /locus_tag="PN977_005230"
     CDS             1609..1818
                     /locus_tag="PN977_005230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000874156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="c1 repressor inactivator"
                     /protein_id="HDI6045133.1"
                     /translation="MAFIPPTIDDVRHCSNALSVDPAETDAARAIAEHYSKISNQEYR
                     ITQDDLDDLTDTIEYLMATNQPDSQ"
     gene            1929..2780
                     /locus_tag="PN977_005231"
     CDS             1929..2780
                     /locus_tag="PN977_005231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000611663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6045134.1"
                     /translation="MINYVYGEQLYQEFVSFRDLFLKKAVARAQHVDTASDGRPVRPV
                     VVLPFKETDSIQAEIDKWTLMARELEQYPDLNIPKTILYPVPNILRGVRKVTTYQTEA
                     VNSVNMTAGRIIHLIDKDIRIQKSAGINEHSAKYIENLEATKELMKQYPEDEKFRMRV
                     HGFSETMLRVHYISSSPNYNDGKSVSYHVPLCGVFICDETLRDGIIINGEFEKAKFSL
                     YDSIEPIICDRWPQAKIYRLADIENVKKQIAITREEKKVKSAASVTRSRKTKKGQPVN
                     DNPESAQ"
BASE COUNT          811 a          654 c          579 g          739 t
ORIGIN      
        1 agctggccca aatgttgctg gatagttttt actgccagac cgcgcgcctg aagatataga
       61 agataatcgc gaacatcttc aggttctgcg ggaaagcatt tccggttatt caacttgcac
      121 catgccgccc acgaccggca aacggacaga agcattttcc aggtatgctc agaaaacgcc
      181 tggcgatccc tgaacatgtc catcaggttc ttgcgaacct catcactcgt tgcatcgacc
      241 ggtaatgcag gcaaattttg gtgtacggtc aggacattag acattttaag tcacacacca
      301 accagacttt cgagaggaat gccaaactgt acgtgcaaac gcctaatcat tggtaacgtt
      361 aaatttcgcg ttccattcag aacttcgtaa acacggttcg acttaccaat cgccggttcc
      421 agatctttca ctgtcaaccc ttgctgttcc atacggaact taatcgcttc aatgggtgta
      481 ggcggttcaa ttgggtaatg tcttttttca tactcttcaa taagcaggca cattacctca
      541 aagaaatcac cttccggggt gttcatttcc ggttcgttat cgaacatagg ttcgaccgcg
      601 cgtagtgcgg cctgataatc ttgttctgta cggataggtt taatgttcat gatttactcc
      661 agctctattg tatccgcgtc gatggcatcg tattccttat gtgttccaat aaacttaacg
      721 aacatccatc ctcgctgata agcaattgca acaattaatc tgtagtcatt acctttgatg
      781 ttgaagacaa cccgcctgtt tttaagaatg ctggcagatc ggtactgcgc tttgatatca
      841 gccgggcttt tccaggtggc cttggagact tcgtcaaccc aggcctttaa tggttgttct
      901 gcatcgggat tcacagacca aaaatccctc aatgttttta ccgaaataat cttcattaca
      961 tattccttat cccggcaagt atattagtcc cattctggga ttaggtaaag ctttttagtc
     1021 ccatcttggg atatatgcgc tacgcgaaat ctggagcact aacactgcca acatttcgca
     1081 gattttacgt agcgcaacct tgatcaaatg atcaagtgat cactatttga cctgataagg
     1141 tattgaactg tatggattta caggtaaatt gatcatgttc aataaccctt aagataactt
     1201 cgtataatgt atgctatacg aagttatcag gtccgaagag gagtttacgt ccagctgcgc
     1261 ataaaaatca agaattatta gagcaataaa atttgagaga aaaatcccac tccaccagcc
     1321 aaaaactgga ttgtttttca tagttgtttg acaattgctc taataaatta tagttttgcc
     1381 gccgtttcgt aatacgactt tggattcact atttaatgtg tcttcagcgt tgtagagcgg
     1441 ctcagaagga aatgagcaaa cagggaaacc ttatacaacg gcattacagc tatgcattgc
     1501 tcatcttaca cacagcgcaa tgttgttaga ttaccccagc atggatcatg ggtgaaacag
     1561 taggtcagag cttcaggctc tgtgttgtca atacagtgag gcataattat ggctttcatt
     1621 ccaccaacca tcgacgacgt tagacattgc tctaacgctt tatctgtaga ccccgccgaa
     1681 accgacgctg cccgcgccat tgctgaacac tactcaaaga tatccaatca ggagtaccgc
     1741 atcacccaag acgacctgga tgatctcact gacacaatcg aatatctcat ggccactaac
     1801 cagccagact cacaataaat gcactaataa atctattatt ttcgttggat ccttctataa
     1861 tggtggccaa caactcccag tgtaatccgc tgtgagttgt tggccatgtc aattctggag
     1921 gaggatcaat gataaattat gtctacggcg aacaactgta ccaggagttc gtcagcttca
     1981 gggatctctt tctaaaaaaa gctgttgcac gcgcccaaca cgttgataca gccagcgacg
     2041 gtcgtcctgt acgcccggtt gtcgttctac cgttcaaaga aactgacagc attcaggctg
     2101 aaattgataa atggacttta atggcgcggg aactggaaca gtacccagac ctcaatatcc
     2161 caaagactat tttatatcca gtgcctaaca tccttcgcgg tgtgcgcaag gttacaactt
     2221 atcagacaga agctgtgaac agcgtcaaca tgaccgctgg ccgcattatt catctgattg
     2281 ataaggacat tcgcatccaa aaaagcgcgg ggatcaatga gcacagtgcg aaatacatag
     2341 agaacctgga agcaacaaaa gagctaatga agcagtaccc ggaggatgaa aaattccgta
     2401 tgcgcgtaca cggctttagc gaaacaatgc tgcgcgtcca ttacatttcc agtagcccta
     2461 actacaatga tggtaaatca gttagttacc atgtgccact gtgtggcgtg tttatctgcg
     2521 atgaaactct ccgtgatgga attatcatca acggtgaatt cgaaaaagca aaatttagcc
     2581 tttatgactc catagaaccg atcatctgcg accgctggcc gcaagcaaaa atatatcgcc
     2641 tggcagatat tgaaaatgta aaaaaacaaa ttgccatcac tcgcgaagag aaaaaggtta
     2701 agtcagccgc atcagttacg cgcagccgta aaaccaagaa ggggcagcca gtaaacgaca
     2761 accccgaaag cgcgcaataa att
//
LOCUS       DAMZFT010000286         2747 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.287, whole genome shotgun sequence.
ACCESSION   DAMZFT010000286 DAMZFT010000000
VERSION     DAMZFT010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2747)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2747)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2747
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.287"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            1..897
                     /locus_tag="PN977_005232"
                     /pseudo
     CDS             1..897
                     /locus_tag="PN977_005232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013317439.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(956..1374)
                     /locus_tag="PN977_005233"
                     /pseudo
     CDS             complement(956..1374)
                     /locus_tag="PN977_005233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558235.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            2175..2675
                     /locus_tag="PN977_005234"
     CDS             2175..2675
                     /locus_tag="PN977_005234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020231635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDI6045135.1"
                     /translation="MENIHSLEKIIKYQQNKDPLYPCQEHLLIQLCIRANKKIQDNIS
                     RMLGGYGINYSTYMVLTVLSTADNHCLSPSEISRELQFTRTNITRITDFLEKAGYTER
                     TDSKEDRRAKKISLTSEGLSFIQGITLTQNQYLKEIWSPLTYDERELFKDINQKLLTH
                     FADVRS"
BASE COUNT          834 a          614 c          586 g          713 t
ORIGIN      
        1 aaaagacccg ggcatcagca tcagtcacct gaacaacaga gaaaaaacaa agattactga
       61 tgctctgaga taaacatatc ccctgacaga attactgcat gttctgggcc ttgcccgtag
      121 cagttatttt tatcaccggg ctgcactgaa agccagtgat aaatacgcca cgatacgtac
      181 gatgctgaca gatatattta acagtaatta ccagtgttat ggctatcgtc gcctgcatgc
      241 gatgctcagg catgaggggg atcggctatc agaaaaggtt gtacgcagac ttatggtgga
      301 ggaacagctt gtcgtcagcc gtaaccgtca tcgccgctac agctcatatt gcggagaaat
      361 cggaccttct ccggataacc ttatcgccag agattttaag gcggagcaac ctaatcagaa
      421 atggctgaca gatatcacgg agttccagct tcctgcagga aaagtctggc tatcaccggt
      481 ggtggactgc ttcgacagaa aagttgtgag ctggtctctc agtacacgcc ccgatgctga
      541 actggtcaac actatgctgg ataacgctgt cgaaacgtta aatgctggcg aacgaccggt
      601 gatacacagt gacagaggtg ggcattatcg ctggcctggc tggaaagagt gaatacagca
      661 ggccttattc gctcaatgtc ccgtaaagga tgctcgcctg ataatgctgc atgcgaaggc
      721 tttttcggca gactgaaaac tgaaatatat tatgggcgta aatgattggg tatcacgcct
      781 gaaaaattca tgcggcatgt ggatgcttac atcagatggt ataacgagcg gcgtataaaa
      841 ttttcgctgg gtgcagtcat ccctgaaatg taccgccaac aatgcgggct ggcataataa
      901 agcagtccag gaaatcgtcc gaatcctaat tactgtctta tatcattggc gctgatacat
      961 taacaggacg cttcacctgc gcgtaataaa cgctacggtg aacaccgaat aaatgacaaa
     1021 taatccgcac tggccgcttt gctttcagca gtgtgattag cgcgacagct tgccggggat
     1081 ttcgctcatc agcacggcag cctgctttag tatttctttt tccatctcaa ggcgctttat
     1141 ctgcgctttt aagctgctga atttctcgct gttcaggggt atggcattac cagcgggctc
     1201 aataccctga agttccagct tatacaaccg tatccattta cgcaaatggt caggattgag
     1261 ctcaagtgcc agcgcgactt ctctgacatc gcgctggtat ttaactactt gctcgatagc
     1321 ttcgagcctg aactccgggg aaaaggtgcg tttagtccga cgagttttgc tcatgcatca
     1381 ccccattttc actgttttaa cattaacagg atttcgaggt gtcctgaatt accgatccac
     1441 tacattaatt cttactggtt aatgattaac aatatgaatc attacatacg cctgttgtat
     1501 atattttagc ctctctttta ccaaacataa gaaaactaaa caggtggcac cgcccctgct
     1561 ggcccgacag agaaaatatc tccttatcac tgctgcatga gtggtacaaa tcagaaaaaa
     1621 aactctcagt gatacgactg gctgaactcc gtgtaaaaat gcattgtgta cgatttacga
     1681 aaaacacact gccacacatc tggaatttac aatgtccgga gagtgtggag aaacgaataa
     1741 agacagttac ggggcaagag ttgcatacag tcgcctcttc tgtaaaactc cacacactgg
     1801 aacagagaag gttctggtaa acatccatcc cggatacaca tatccaccat gctatggaaa
     1861 tcaggatatt accgtgcact gaataaaata atattgcatg aatattcagg gccgtcagga
     1921 ttcgttcaac aaagcacagg ggcagataga ttatctatac tgatttaaca gtataaataa
     1981 ttctttcacc atcttgtcag cgatgaacat ttatgttctt ttatttgaag gaatctgctc
     2041 ttactctctg ccgggccgcc tcactcatac agatatcagg cagtcataga ttgttgtgaa
     2101 aagtagatat ttaccggatg actgatgcac ttttatataa aggcagaatg tttcagtata
     2161 tggaaaatga ttaaatggaa aacatacact cattagagaa gataatcaaa tatcaacaaa
     2221 acaaagatcc cctgtatccc tgtcaggaac atcttttgat acaactctgt atccgtgcaa
     2281 acaagaaaat acaggataat atatcgagaa tgctgggagg atacggaata aattactcaa
     2341 catatatggt tctcaccgta ttatctacag cagataatca ttgtctttcg ccttcagaga
     2401 tcagcagaga actccagttt accagaacaa acattacccg cattacagat tttttagaaa
     2461 aagccggata tacagaaaga actgatagca aagaggaccg ccgtgctaaa aaaatcagtc
     2521 tgacatctga aggcctgtcc tttattcagg gcattaccct tacacaaaac cagtacctga
     2581 aagaaatctg gagtcctctg acctatgatg aacgtgaact gtttaaagac attaatcaaa
     2641 aattactgac gcattttgct gatgtacgtt cataaagtat gaaaagctgt cacgaacggt
     2701 gcaatagtga tccacaccca acgcctgaaa tcaggttctt ccgcaac
//
LOCUS       DAMZFT010000287         2733 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.288, whole genome shotgun sequence.
ACCESSION   DAMZFT010000287 DAMZFT010000000
VERSION     DAMZFT010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2733)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2733)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2733
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.288"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..1370
                     /locus_tag="PN977_005235"
     CDS             <1..1370
                     /locus_tag="PN977_005235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HDI6045136.1"
                     /translation="AIISILLSKKTVTTRKNYISLFLLIIITIPFSFYPYIGTIFSPN
                     NRISEIINSSANNLNIKYIYWNWSQNIGVNGLPMHIIQTSVRKSVPHATDAEQLRYLD
                     EKRSLVSTHIKNKTIIYVLCESCWYDKKNFRENFKPVIDEGYSELRAISPVYGGGTAN
                     AEFEMLTGLPSNSDKISGIIYQEYSDAFKPKVDALPNALKNKGYITFAAHNYQADFWY
                     RNKIYPKFGFDRFDSIINMGDLPPEYSSIKKPWQWQPDDYLLYNAALKAIQNAGNKDI
                     FVHLITMSTHGPYQHINDYGEGVYTYEINEAVKRFVQFSQQVEKIDPNAVIVFYGDHK
                     PPLNKYFVENGVLPSNIFNKIGEEKDEDFVFKMSTTPLDFGDVPVLIKSNDKDAIKKF
                     ITDANGKPFFCVSSLVDKHFIQSGLVSFNHNAKHVCENSDNFNYDKLINMTPSWIYSM
                     SLFHD"
     gene            1622..1702
                     /locus_tag="PN977_005236"
                     /pseudo
     CDS             1622..1702
                     /locus_tag="PN977_005236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461174.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DinI family protein"
     gene            1796..2023
                     /locus_tag="PN977_005237"
     CDS             1796..2023
                     /locus_tag="PN977_005237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000497451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI family protein"
                     /protein_id="HDI6045137.1"
                     /translation="MFVELVYGKRNFDGLPGAKDIILGELTKRVHRIFPGADVRVKPM
                     MTLPAINTDANKHENEQISRTVQEMFEETDM"
     gene            complement(2112..2480)
                     /locus_tag="PN977_005238"
     CDS             complement(2112..2480)
                     /locus_tag="PN977_005238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000843044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber domain-containing protein"
                     /protein_id="HDI6045138.1"
                     /translation="MKSTGNAVAPGAWVPNSDGRLKDGVSRIGDPLSKMEMLKGCSWT
                     RKDTGQWGIGFIAQDVKKIFPQAVTEGGDRQLPDGTMVEGVLSPDTYGVAAALHHEAI
                     LALMNELSELREEVNALKSE"
     gene            2576..>2733
                     /locus_tag="PN977_005239"
     CDS             2576..>2733
                     /locus_tag="PN977_005239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDI6045139.1"
                     /translation="MIFSPQHKTGDLMNKKTKRTFTPEFRLECAQLIVDKGYSYRQAS
                     EAMNVGSTT"
BASE COUNT          882 a          561 c          500 g          790 t
ORIGIN      
        1 gcgcaataat ctcaatccta cttagcaaaa aaacggtcac aacaagaaaa aattatatat
       61 cgttatttct tttaattatc attacgatcc ccttttcatt ctacccctat attggaacca
      121 tttttagccc taacaatagg atttctgaaa tcataaactc cagcgcgaac aacttaaata
      181 taaaatatat ttactggaat tggtctcaaa atattggcgt taatggcttg ccaatgcata
      241 taatacaaac aagtgtcaga aaatctgtac cacatgcaac tgatgcagaa caattaagat
      301 atttagatga aaaaagatct ttagtatcca ctcatataaa aaataaaacc attatttatg
      361 ttttatgtga atcatgttgg tatgataaaa aaaacttccg agaaaatttc aaacctgtta
      421 ttgatgaagg ttacagtgaa ttacgggcca tctcaccagt ttacggtgga ggtaccgcaa
      481 atgccgaatt tgaaatgctg acaggactac caagtaattc agataaaata tcaggaatca
      541 tctatcaaga atattcagat gcattcaaac caaaagttga cgcattacct aatgccctga
      601 aaaacaaagg ctatattact ttcgcagcac ataattacca agcagacttt tggtatagga
      661 ataaaattta ccctaaattt ggatttgata gatttgatag cattattaat atgggagatt
      721 taccaccaga gtatagcagt attaaaaaac catggcagtg gcaacctgat gactacctgc
      781 tttacaatgc agcattaaaa gccatccaaa atgcaggaaa caaagatatt tttgtacatt
      841 tgatcactat gtcaactcat ggtccttatc aacacattaa tgattatggt gaaggtgttt
      901 atacctatga gattaatgag gcagtcaaaa gattcgttca attttcacag caagtagaaa
      961 aaatagatcc aaatgctgta atcgtctttt acggtgacca taaaccacca ctaaataaat
     1021 atttcgtcga aaatggggtg ctaccaagta atattttcaa caaaatcgga gaagaaaaag
     1081 atgaagattt cgtctttaaa atgagcacaa ccccactcga ttttggtgat gttcctgtac
     1141 ttattaaatc aaatgataaa gacgccatta aaaaattcat aaccgacgcc aatggaaaac
     1201 cttttttctg tgtatcttca ttagttgata aacatttcat tcaatctgga ttggtatcat
     1261 ttaatcacaa cgcaaagcat gtctgtgaaa acagtgataa ctttaattat gataagttaa
     1321 ttaacatgac cccgtcgtgg atttattcaa tgtcattatt tcacgattag tatctagaaa
     1381 ataattgtaa taaatcatca ttaattatgt agacgcctaa aattaggcat ctacatttta
     1441 ttagaaaaga ggttgtcctg actaggttgt ggaaatgtgc atcgcataaa tcaaggctat
     1501 agagcatgaa agtgcgttct tccccctcta ccattgataa atatttaaaa gtgctcacgc
     1561 tggattcgat ccaggcatta gcatctttga gtgagaattc ccagttaact ttgctcaacc
     1621 cctgaacaaa tcgctgtgta gtgacggtct tccggccatt cggctcttgc tgtatcgaag
     1681 aataccaggc tatttcaata tcgctgcgtc gtggcatcat tacgccctct cttgaatacc
     1741 ggataaaaac acagtataaa tactgtatat acacccagta aagaggcaat gagcaatgtt
     1801 cgtggaactc gtttatggca aaaggaattt tgatggactg cccggtgcaa aagatatcat
     1861 tctgggcgag ttgaccaaga gggttcaccg gattttcccc ggcgctgatg tccgggttaa
     1921 accgatgatg acattgccgg cgatcaacac tgacgccaac aagcatgaga atgaacagat
     1981 aagccgtact gttcaggaaa tgtttgaaga gactgatatg tagctgtttt cagattaaac
     2041 gccttgaacc gtcacactga ttaagtacaa tccggcgtgg ttgcggcttc cagattataa
     2101 gaatgattcc cttactcact tttcagcgca ttcacttctt ctcgtaattc actgagttcg
     2161 ttcattagtg ccagaatcgc ttcatgatgc aacgctgccg caacgccgta agtatctgga
     2221 gagagtactc cttccaccat agttccatca ggaagttgac gatcacctcc ctcagttact
     2281 gcctggggga agattttctt aacatcctgc gcgataaacc caatccccca ttgtccggta
     2341 tcttttcgcg tccaggaaca accttttagc atttccatct tactgagcgg gtctccaatt
     2401 ctggacactc catctttcag gcggccatct gaattcggaa cccatgctcc aggcgctaca
     2461 gcgttaccag ttgatttcat aaagctccat gtggttcctg agcttgcgtt tgctacctga
     2521 atattccctg ataaagaact gtagtggcac agtaaatttg gccacctgat taaaggtgat
     2581 attctcacct caacacaaaa caggtgactt aatgaacaag aaaaccaaac gtactttcac
     2641 ccctgagttc aggctggaat gtgcacagct gattgttgat aagggctatt cgtatcgaca
     2701 agccagtgag gcgatgaatg tcggttctac cac
//
LOCUS       DAMZFT010000288         2727 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.289, whole genome shotgun sequence.
ACCESSION   DAMZFT010000288 DAMZFT010000000
VERSION     DAMZFT010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2727)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2727)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2727
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.289"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..687
                     /locus_tag="PN977_005240"
     CDS             <1..687
                     /locus_tag="PN977_005240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001075464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus Gp2 family protein"
                     /protein_id="HDI6045140.1"
                     /translation="SLLDSALAQHRAVSLIRVDLRFPEYMPATIMDPDPDSAVISRFF
                     ESLKAKIQAYQQHKRRASQRVHATSLRYFWCREFGEEENRKHYHVILLLNKDTWCSLG
                     DFSEPSSLAAMIQEAWCSALHLEPWQGDGLVHFSRWKPFRKPASSDARPSSDDTPLSG
                     GCSDTRKASEKKPGEAAVLWIKRGDVEALQKARNRASYLVKYETKQHDGSGQRNYGCS
                     RGPGRLLDGR"
     gene            1024..1599
                     /locus_tag="PN977_005241"
     CDS             1024..1599
                     /locus_tag="PN977_005241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6045141.1"
                     /translation="MNTFSYFDDSMAPFWPSYQNKVIGVLDRALREQSGSRIRRILLR
                     LPWEPDNTFSSRRTWFGMAFIETVSDLMNATPGRDLCWLLTRHPEKPEYHVVLCVRQE
                     YFDGPELDRLILDAWSNVLGFASPGEAAPYQKQITRDVLLDPRSPDCEELFRDLIWAF
                     SDFARDRRGVYDPEARHLAGNPWYPGSAGRR"
     gene            1667..1900
                     /locus_tag="PN977_005242"
     CDS             1667..1900
                     /locus_tag="PN977_005242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000958486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family transcriptional regulator"
                     /protein_id="HDI6045142.1"
                     /translation="MSTTTSHDSVWLRDDDPLIDMSFITRFTGMTDKWFYQLMSEGRF
                     PKPIKLGRSSRWYRSEVEQWMQQRIEASRGIAA"
     gene            2100..2609
                     /locus_tag="PN977_005243"
     CDS             2100..2609
                     /locus_tag="PN977_005243"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6045143.1"
                     /translation="MQITETVLSEPVEIRRFIQQAVDHWPRLLAVHFTLHSTEGNRDE
                     GQIHAFYRAFYRQLNQRSHAGYPLLPVVLRWLRETTESTGIRCLLLISQDLICPPGAG
                     LTADDADSHLVALLQQTGSTLTPEGHCTVVCCYRVVRAATPECAESFTTFRNTCLSLS
                     RPVMAGIIR"
BASE COUNT          587 a          760 c          745 g          635 t
ORIGIN      
        1 agccttctcg acagtgccct ggcgcaacac cgtgccgttt cgttaatccg ggtggattta
       61 cgttttcctg agtatatgcc tgccaccatt atggatcccg atccggattc agcggtgatt
      121 tctcgttttt tcgaatccct gaaagccaaa atccaggctt accagcagca taagcgacgt
      181 gccagtcagc gtgtgcatgc aaccagcctg cgttatttct ggtgccggga gttcggagag
      241 gaagaaaaca ggaaacatta tcacgtgata ttgctgctca acaaagatac ctggtgctcg
      301 ctcggggatt tcagcgaacc gtcttcgctg gcggcgatga tccaggaggc atggtgcagc
      361 gctctgcatc ttgagccctg gcaaggcgat ggcctggtcc atttttccag gtggaagcct
      421 tttcgtaaac cagcatcgtc tgatgctcgc ccttcttccg atgacacgcc tttgtcgggt
      481 ggatgttctg ataccaggaa ggcttcagaa aaaaagccgg gtgaagccgc tgttctctgg
      541 atcaagcgtg gtgatgtgga agcgctgcag aaagcccgga acagagccag ttatctcgtg
      601 aaatatgaga cgaagcagca tgacggttcc gggcaacgta attatggttg cagtcgtggg
      661 ccggggcgtc tgctggatgg caggtgagcc ctgtaaaacg acatccggtg cctgagctta
      721 tgctacagga cgttggctga cgcccttagc tcgttttctg aaaaagtcgt cctgaagtca
      781 tgcaacggta tttttctctg ctttcgctcc tggaaacact ccgtcggtaa aaccttaccg
      841 ccctgcttaa tgatgtgaac cgaagtcaac ggagttcatt caccctgaat gttcttccct
      901 gatattcccg ctcagcgtcg gttcttcacc tgttccatca acctcatccg cagcctgtaa
      961 cccgtcagcc catccatccc cgggcaggac gcgttgtggg ctgcgcataa cggagcatat
     1021 cctgtgaata ctttctctta tttcgacgac agcatggcac ccttctggcc ctcgtatcag
     1081 aacaaagtta ttggcgttct tgaccgtgcg ctgcgtgagc agtccggctc ccggatacga
     1141 cgtatcctgc ttcgtctgcc gtgggaaccc gataatacct tcagcagccg caggacctgg
     1201 ttcggtatgg cctttattga aaccgtcagt gacctgatga atgcaacccc cggacgtgac
     1261 ctctgctggc tcctgacacg tcatcctgaa aagccggaat accacgtggt gctgtgcgtc
     1321 aggcaggagt atttcgacgg ccctgaactg gatcggctga ttctggatgc ctggagcaac
     1381 gtgctgggct tcgcctcacc gggggaagcg gcaccgtatc agaagcagat tacccgggat
     1441 gtgctgctgg atccccgttc accggactgc gaggaactgt tcagagacct gatctgggcg
     1501 ttcagtgatt tcgcccgtga tcgccgtggt gtgtacgatc cggaagcacg ccacctcgcc
     1561 ggtaatccct ggtatcccgg atcagcagga cggcgctgaa catcatgttc agccgttccc
     1621 ccgcacttac acatccttcc atttcaccga ggatattccg accatgttga gcacaacaac
     1681 atcccacgac agcgtatggc tgcgtgacga cgatcccctg atcgacatga gcttcatcac
     1741 ccgcttcacc ggcatgaccg acaaatggtt ctaccagctg atgagtgaag gcaggttccc
     1801 gaagcccatc aaactgggcc gcagcagccg ctggtacaga agcgaagtgg agcagtggat
     1861 gcagcagcgc atcgaagcat cacggggaat cgcagcatga aaggtcttgt gataccggtt
     1921 cgctggcaat gtgcaaactg ccggcgctgg tattgtggta atcccccctg cccctggtgc
     1981 tggcggcctg ctgcaccgtc tgtcattcct catccttcgc tgtaacaggc atcgtactgg
     2041 tgccggcgtg ctttcctgcg taccggcacc tgttcagaaa cattcaaaga gagcaaatcg
     2101 tgcagataac cgaaaccgta ctttcagagc cggtggaaat ccggcgtttt attcagcagg
     2161 cggtggatca ctggccgcgt ctgctggccg ttcatttcac gctccattca acagaaggaa
     2221 atcgtgacga ggggcagatt catgcgtttt accgggcatt ttaccgacag ctgaatcagc
     2281 gaagccatgc cggatatccg ttactgccgg tggtactacg ctggctgcgg gaaacgacag
     2341 aaagcaccgg gatacgctgt ctgttgctga tcagtcagga tctcatttgc ccaccgggag
     2401 ccgggttaac agctgatgac gctgattctc acctggtggc gttactgcag cagaccggca
     2461 gcacactcac accagaaggg cactgtacgg tggtatgctg ttacagagtg gtgcgtgcag
     2521 ccacacctga atgtgctgag tctttcacga cattcagaaa cacgtgcctt tcgttatcca
     2581 ggcccgtgat ggccggcatc atccgctaag ccattcgcga tgtcatcccc tccggtacag
     2641 gtttcaggct gtggctgcac cggtgactga aacgatcacg gctggtatta ttaaaaaaga
     2701 gtgataccgg acagttgagt gaatgac
//
LOCUS       DAMZFT010000289         2677 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.290, whole genome shotgun sequence.
ACCESSION   DAMZFT010000289 DAMZFT010000000
VERSION     DAMZFT010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2677)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2677)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2677
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.290"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..1456
                     /locus_tag="PN977_005244"
     CDS             <1..1456
                     /locus_tag="PN977_005244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HDI6045144.1"
                     /translation="GHYKAYILGEGDDKTAKTPQWASQITGIPVDRIIKLAREIGTAK
                     PAYICQGWGPQRQANGELTARAIAMLPILTGNVGISGGNSGARESTYTITIERLPVLD
                     NPVKTSISCFSWTDAIDHGPQMTAIRDGVRGKDKLDVPIKFIWNYAGNTLVNQHSDIN
                     KTHEILQDESKCEMIVVIENFMTSSAKYADILLPDLMTVEQEDIIPNDYAGNMGYLIF
                     LQPVTSEKFERKPIYWILSEVAKRLGPDVYQKFTEGRTQEQWLQHLYAKMLAKDPALP
                     SYDELKKMGIYKRKDPNGHFVAYKAFREDPEANPLKTPSGKIEIYSNRLAEIARTWEL
                     EKDEVISPLPVYASTFEGWDSPERRTFPLQLFGFHYKSRTHSTYGNIDVLKAACRQEV
                     WINPIDAQKRGIANGDMVRVFNHRGEVRLPAKVTPRILPGVSAMGQGAWHEANMSGDK
                     IDHGGCVNTLTTLRPSPLAKGNPQHTNLVEIEKI"
     gene            1517..2677
                     /locus_tag="PN977_005245"
                     /pseudo
     CDS             1517..2677
                     /locus_tag="PN977_005245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416105.4"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
BASE COUNT          735 a          666 c          681 g          595 t
ORIGIN      
        1 tggtcactat aaagcctata ttcttggtga aggtgatgat aaaacagcga aaacgccgca
       61 gtgggcttcg caaattaccg gtatcccggt ggaccgtatc atcaagctgg ctcgtgaaat
      121 tggcacagca aaacccgctt atatctgcca gggctggggg ccgcaacgcc aggcaaacgg
      181 tgagctgacc gcacgcgcta ttgctatgtt gcctattttg acgggcaatg tcggcatcag
      241 cggcggaaat agtggtgcgc gtgaatcgac ctataccatt accatagaac gcctaccggt
      301 gctggataat ccggtcaaaa cgtcaatctc ttgcttcagc tggacggatg ctatcgatca
      361 tggcccgcaa atgacggcaa tccgcgacgg tgtccggggc aaagataaac tggatgtgcc
      421 cattaagttc atctggaact acgcgggaaa taccctcgtt aatcagcatt ctgacatcaa
      481 caaaacgcat gaaattctgc aggacgaatc gaaatgcgaa atgattgtgg tcatcgaaaa
      541 ctttatgacc tcatcggcaa aatatgccga cattctgctg ccagacctga tgaccgttga
      601 gcaggaagat attattccta acgactacgc cggtaacatg ggatatctca ttttccttca
      661 gccagtcacc agcgaaaaat tcgaacgcaa accgatttac tggatcctga gtgaagtcgc
      721 gaaacgtctt ggacctgacg tctatcaaaa gttcactgaa ggtcgcactc aggaacaatg
      781 gttacaacat ctgtacgcca aaatgcttgc caaagatccg gcgttaccgt cttacgacga
      841 actgaaaaaa atgggtatct ataagcgtaa agatcccaat ggtcattttg tcgcctacaa
      901 agcatttcgt gaggaccccg aggcaaatcc acttaaaacg ccttccggta agattgaaat
      961 ttattccaac aggctggcgg aaattgcccg tacctgggaa ctggaaaaag atgaagtgat
     1021 aagcccattg ccagtttatg cctcaacctt tgaaggctgg gactcccctg aacgtagaac
     1081 cttcccactg caactgtttg gtttccatta caaatcccgt actcactcga cctacggcaa
     1141 cattgatgtc ctgaaggcag cctgccgtca ggaggtgtgg atcaacccta tagatgcaca
     1201 gaaacgtggg attgccaacg gcgatatggt gcgggtgttt aaccatcgtg gcgaagttcg
     1261 gctaccagcc aaagtgacac cacgcattct ccctggagtt agtgctatgg gccagggagc
     1321 ctggcacgag gccaatatgt ctggcgataa aatcgaccat ggcggctgtg tgaatacgct
     1381 aaccactctg cgcccttcac cactggcgaa gggaaacccg cagcacacta atctggtcga
     1441 gatcgaaaaa atataaccct ctacgaccat aaattttgac atgacatttt gtttgaaaag
     1501 caataagtga gtaatgatga aaatccatac cacagaggcg ctcatgaagg ctgaaatcag
     1561 ccgtagaagt ctgatgaaaa cctccgcact tggcagtctt gcgctggcaa gcagtgcttt
     1621 cactctgcca ttttcccaaa tggtccgagc ggcagagaca ccggtagaag agaaagcggt
     1681 ctggagttcc tgcaccgtta actgcgggag ccgctgtctg ttacgtttgc atgtgaaaga
     1741 tgacaccgtg tactgggtgg agtctgatac gacaggtgac gacgtctacg gtaatcatca
     1801 ggttcgagcg tgtttacgcg ggcgctctat ccgtcgacgg atgaatcatc ctgacaggtt
     1861 gaaatatccc atgaagcgcg tcggcaagcg cggtgaaggt aaatttgaac ggataagttg
     1921 ggatgaagcc ctggatacca tcagtgataa tcttcggcgg atcctgaaag attacggcaa
     1981 tgaggctgta catgtcctgt acggaacagg cgtagatggc ggaaacatca ccaactcgaa
     2041 cgtcccgtac cgtctgatga actcttgcgg tggttttctc agtcgctatg gcagctacag
     2101 taccgcacag atcagtgccg cgatgagtta tatgttcggt gccaatgatg gcaacagccc
     2161 ggatgatatc gccaatacga aactggtcgt tatgttcgga aataacccgg cagaaacccg
     2221 gatgagcggc ggtggtgtca cttactacgt cgagcaagcc cgcgaacgtt caaacgcacg
     2281 catgatcgtc attgatccac gttataacga cactgctgcc ggcgtgaaga tgaatggctg
     2341 cccattcgcc ctggcaccga tggcgcactg gcctgtgcga ttgcctgggt actgattact
     2401 gaaaacatgg tcgatcagcc atttctcgac aaatattgtg ttggttacga tgaaaaaacg
     2461 ctgcccgcca acgcaccacg taacgcgcat tataaagcct atattctggg cgaagggcct
     2521 gacggcatag ctaaaacgcc ggaatgggca gcaaaaatca ccagcatccc ggcagaaaaa
     2581 attatccagt tggcacgaga gatcggttca gcaaaaccgg cttatatttg tcagggttgg
     2641 gggccacaac gacattccaa cggcgagaaa acatccc
//
LOCUS       DAMZFT010000290         2641 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.291, whole genome shotgun sequence.
ACCESSION   DAMZFT010000290 DAMZFT010000000
VERSION     DAMZFT010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2641)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2641)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2641
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.291"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..355
                     /gene="sitD"
                     /locus_tag="PN977_005246"
     CDS             <1..355
                     /gene="sitD"
                     /locus_tag="PN977_005246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="iron/manganese ABC transporter permease subunit
                     SitD"
                     /protein_id="HDI6045145.1"
                     /translation="SGLHTRWLHYGLLCMVSLTIVATLKAVGIILSISLLIAPGAIAV
                     LLTQRFHIALLLATGISILVSMTGVWLSFFIDSAPAPTIVVLFAVMFIMTFTVTSINA
                     RTKENAEPRDLLSSD"
     gene            complement(589..846)
                     /locus_tag="PN977_005247"
                     /pseudo
     CDS             complement(589..846)
                     /locus_tag="PN977_005247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611787.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
     gene            844..1257
                     /locus_tag="PN977_005248"
                     /pseudo
     CDS             844..1257
                     /locus_tag="PN977_005248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015377870.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
     gene            1598..1978
                     /gene="crcB"
                     /locus_tag="PN977_005249"
     CDS             1598..1978
                     /gene="crcB"
                     /locus_tag="PN977_005249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001318207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HDI6045146.1"
                     /translation="MIKSLFAVIIGGSVGCTLRWLLSTRFNSLFPNLPPGTLVVNLLA
                     GLIIGTALAYFLRQPHLDPFWKLMITTGLCGGLSTFSTFSVEVFALLQAGNYIWALTS
                     VLVHVIGSLIMTALGFFIITILFA"
     gene            complement(2358..>2641)
                     /locus_tag="PN977_005250"
     CDS             complement(2358..>2641)
                     /locus_tag="PN977_005250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDI6045147.1"
                     /translation="EQLWLFYLGCAVLSFGAALATPAYQLLLNDKLADGAGAGWLAAS
                     HTLGYGLCALLVPLASKTGVSIALIVTALFAAVLFTIVSACIWHYRTIK"
BASE COUNT          682 a          601 c          606 g          752 t
ORIGIN      
        1 aagcggtttg catacgagat ggctgcatta tgggctgctg tgcatggtct ccctgaccat
       61 tgtggcgacg ctgaaagcgg taggtatcat tctctctatt tctcttctca ttgcgcctgg
      121 tgccattgcg gtactcctga cacaacgctt tcatattgcg ctcctgctgg cgactggcat
      181 atcgatattg gtctcaatga ctggtgtctg gctgtccttt tttatcgaca gcgcgccagc
      241 tccgacaatt gttgtcttat tcgcggtcat gttcatcatg acgtttaccg ttaccagcat
      301 caacgcacgc acaaaggaaa atgccgaacc acgggatttg ttatcatccg attagcagaa
      361 cactcggcca gcaacatccg cttattggcg atgcgcttga acgaataatg cacagactta
      421 tgattctgat aagttttgtt gccagagagt tgctgcggac tggatagcaa gtaatgtcga
      481 ttctcagggg tagcaaacag cgcagtaaaa tgagggaagt agtggcaaaa catagagaaa
      541 attggggggc gagggccagt gatgtaaaaa accacgctaa gagtttaatt aattgtttgt
      601 attgaagttt aatgatctta actcaccctt aagaatatct tccggtaacg aagacgtata
      661 atgccccagc atatttatat ggctatgtat cagcagcgat aatcttgccg gatgctactc
      721 ctcaggagtt tctcccgatt tgcgaagatg tgacaacgcc tcctgcatgt aaagtgtgtt
      781 tcacagcaca accgcattgg tcaccagccc cagagcacct agttgatctt cctgccctta
      841 acggtagctg aagatgactg ggctggatgg aaaatcctgc atggtgcatt gggagagcag
      901 atagaattgg ttggcgacga tctgtttgtg accaacgtaa agtatattca gcgcggcatt
      961 gacgaaaacc ttgccaattc agcattgatt aaactcaacc agattggttc cctgagtgaa
     1021 acctttgatg cggtacaact ttgccatgac aacaactggg gcacctttat ctcacaccgc
     1081 agtggtgaga cagtggacag ctttatcgcc gatatgacag ttgcaatgcg cgcaggtcat
     1141 ctcaaaacgg gagctccatg ccggggtgag cgtattgaaa aatataacca gcttatgaga
     1201 attgaggatg aactgggttc atcggcgcaa tttgccggaa agtctgcctt taaatgacat
     1261 tattgtgcca atcccccagg cttacacgtc ggggttattc tggaggaatg atgaaagctc
     1321 acgtttttgt tatttctatg cttactggca ttgtcgtcac ctatgccgtg ttacttcttg
     1381 gttgcttgtt catcgataaa acgctcccca ctgtagatgt tgtcattctg tcacttgtcg
     1441 tgggggcttc tgctcagcaa ttgtcgaggg tattaatgtc aataaataga accttccctt
     1501 atcttgcatg tagtcaaagg atataaatta acaatatcag tcaaatgaga acatattctg
     1561 atattgttac gataaaaaat tattttatga ggggtacatg attaaatcac tttttgccgt
     1621 cattatcggc ggttctgttg gatgtactct gcgctggtta ctgtccacac gctttaacag
     1681 tctttttcca aatctacctc caggtacact ggtggtgaac ctccttgccg ggttaattat
     1741 tggtactgcg ttggcgtact ttttacgaca accacacctt gatccttttt ggaaattaat
     1801 gataacaacg ggactttgtg gaggtctgag tactttctct acattttctg ttgaagtatt
     1861 cgcattactc caggcgggaa attatatatg ggctttgacg tctgtacttg ttcacgtcat
     1921 tggctccctc atcatgacag ctttaggctt ttttatcata accatacttt tcgcatgaga
     1981 attcattaac gttacaggtt tatcaactaa tgtgacgctt aggatagaat ttcgctccat
     2041 ggtatgcgtt tgcagtcatt acagtctcca tgacgactgt aatgactgca aacgcattac
     2101 ctgacatatc agtataaaca atgaattaac ctcttggcgt attttttaca tcactggccc
     2161 tcacccccca aaattctctc tgaaaaattg ttgcggttaa cgttgccaat gtatccaccc
     2221 atttcagaag acgatctggc atcctggtgg catgctgcga tgtgcttcag gtgagtgcac
     2281 tgggcggtca gcacaggcgg tttcgacagc gaaattgcac aggacgttat gccagctgtg
     2341 agtacaacat catagcctta cttgattgta cggtaatgcc aaatacatgc agacactatg
     2401 gtaaataata cagcggcaaa taatgccgtc acaatcagcg cgattgagac acctgttttc
     2461 gacgccagtg gcaccaacaa tgcgcacagc ccatagccaa gtgtgtgact ggccgcaagc
     2521 cagcccgcgc ctgcgccgtc ggccagctta tcattcagta aaagttgata agcgggtgtt
     2581 gccagagcgg ccccaaatga cagcactgca cagcccaggt aaaacaacca tagctgttca
     2641 g
//
LOCUS       DAMZFT010000291         2620 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.292, whole genome shotgun sequence.
ACCESSION   DAMZFT010000291 DAMZFT010000000
VERSION     DAMZFT010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2620)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2620)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2620
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.292"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(23..496)
                     /locus_tag="PN977_005251"
     CDS             complement(23..496)
                     /locus_tag="PN977_005251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001389366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrA17"
                     /protein_id="HDI6045148.1"
                     /translation="MKISLISAVSENGVIGSGPDIPWSVKGEQLLFKALTYNQWLLVG
                     RKTFDSMGVLPNRKYAVVSKNGISSSNENVLVFPSIENALKELSKVTDHVYVSGGGQI
                     YNSLIEKADIIHLSTVHVEVEGDIKFPIMPENFNLVFEQFFMSNINYTYQIWKKG"
     gene            654..1667
                     /gene="intI1"
                     /locus_tag="PN977_005252"
     CDS             654..1667
                     /gene="intI1"
                     /locus_tag="PN977_005252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 integron integrase IntI1"
                     /protein_id="HDI6045149.1"
                     /translation="MKTATAPLPPLRSVKVLDQLRERIRYLHYSLRTEQTYVHWVRAF
                     IRFHGVRHPATLGSSEVEAFLSWLANERKVSVSTHRQALAALLFFYGKVLCTDLPWLQ
                     EIGRPRPSRRLPVVLTPDEVVRILGFLEGEHRLFAQLLYGTGMRISEGLQLRVKDLDF
                     DHGTIIVREGKGSKDRALMLPESLAPSLREQLSRARAWWLKDQAEGRSGVALPDALER
                     KYPRAGHSWPWFWVFAQHTHSTDPRSGVVRRHHMYDQTFQRAFKRAVEQAGITKPATP
                     HTLRHSFATALLRSGYDIRTVQDLLGHSDVSTTMIYTHVLKVGGAGVRSPLDALPPLT
                     SER"
     gene            1870..2220
                     /locus_tag="PN977_005253"
     CDS             1870..2220
                     /locus_tag="PN977_005253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000454193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3330 domain-containing protein"
                     /protein_id="HDI6045150.1"
                     /translation="MEVVAEGVETPDCLAWLRQAGCDTVQGFLFARPMPAAAFVGFVN
                     QWRNTTMNANEPSTSCCVCCKEIPLDAAFTPEGAEYVEHFCGLECYQRFQARASTATE
                     TSVKPDACDSPPSG"
     gene            2346..>2620
                     /locus_tag="PN977_005254"
     CDS             2346..>2620
                     /locus_tag="PN977_005254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000147566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDI6045151.1"
                     /translation="MTGQRIGYIRVSTFDQNPERQLEGVKVDRAFSDKASGKDVKRPQ
                     LEALISFARTGDTVVVHSMDRLARNLDDLRRIVQTLTQRGVHIEFVK"
BASE COUNT          574 a          715 c          719 g          612 t
ORIGIN      
        1 cgactggtgc tgcaacgcat tgttagcctt ttttccaaat ctggtatgta taatttatat
       61 tagacataaa aaactgttca aaaaccaaat tgaaattctc aggcattata gggaatttga
      121 tatcaccttc gacttcaacg tgaacagtag acaaatgaat tatatctgct ttttcaataa
      181 ggctattata gatttgaccc ccgccagaga catatacatg atctgtaact tttgatagct
      241 ctttcaaagc attttctatt gaaggaaaaa ctaggacgtt ttcatttgag cttgaaattc
      301 cgttctttga cactactgca tatttgcgat ttggaagaac acccatagag tcaaatgttt
      361 ttcttccgac aaggagccat tgattatatg tgagcgcttt aaagagtagt tgctcacctt
      421 ttactgacca cgggatatca ggaccactac cgattacgcc attttctgac actgcagaaa
      481 tcaatgatat tttcaattta actcccttaa tggctaactt tgttttaggg cgactgccct
      541 gctgcgtaac atcgttgctg ctccataaca tcaaacatcg acccacggcg taacgcgctt
      601 gctgcttgga tgcccgaggc atagactgta caaaaaaaca gtcataacaa gccatgaaaa
      661 ccgccactgc gccgttacca ccgctgcgtt cggtcaaggt tctggaccag ttgcgtgagc
      721 gcatacgcta cttgcattac agtttacgaa ccgaacagac ttatgtccac tgggttcgtg
      781 ccttcatccg tttccacggt gtgcgtcacc cggcaacctt gggcagcagc gaagtcgagg
      841 catttctgtc ctggctggcg aacgagcgca aggtttcggt ctccacgcat cgtcaggcat
      901 tggcggcctt gctgttcttc tacggcaagg tgctgtgcac ggatctgccc tggcttcagg
      961 agatcggaag acctcggccg tcgcggcgct tgccggtggt gctgaccccg gatgaagtgg
     1021 ttcgcatcct cggttttctg gaaggcgagc atcgtttgtt cgcccagctt ctgtatggaa
     1081 cgggcatgcg gatcagtgag ggtttgcaac tgcgggtcaa ggatctggat ttcgatcacg
     1141 gcacgatcat cgtgcgggag ggcaagggct ccaaggatcg ggccttgatg ttacccgaga
     1201 gcttggcacc cagcctgcgc gagcagctgt cgcgtgcacg ggcatggtgg ctgaaggacc
     1261 aggccgaggg ccgcagcggc gttgcgcttc ccgacgccct tgagcggaag tatccgcgcg
     1321 ccgggcattc ctggccgtgg ttctgggttt ttgcgcagca cacgcattcg accgatccac
     1381 ggagcggtgt cgtgcgtcgc catcacatgt atgaccagac ctttcagcgc gccttcaaac
     1441 gtgccgtaga acaagcaggc atcacgaagc ccgccacacc gcacaccctc cgccactcgt
     1501 tcgcgacggc cttgctccgc agcggttacg acattcgaac cgtgcaggat ctgctcggcc
     1561 attccgacgt ctctacgacg atgatttaca cgcatgtgct gaaagttggc ggtgccggag
     1621 tgcgctcacc gcttgatgcg ctgccgcccc tcactagtga gaggtagggc agcgcaagtc
     1681 aatcctggcg gattcactac ccctgcgcga aggccatcgg tgccgcatcg aacggccggt
     1741 tgcggaaagt cctccctgcg tccgctgatg gccggcagca gcccgtcgtt gcctgatgga
     1801 tccaacccct ccgctgctat agtgcagtcg gcttctgacg ttcagtgcag ccgtcttctg
     1861 aaaacgacaa tggaggtggt agccgagggt gtggaaacac ccgactgcct tgcgtggttg
     1921 cggcaggcgg gttgcgacac ggtgcagggt ttcctgttcg ccaggccgat gccggcggcg
     1981 gccttcgtcg gcttcgtcaa ccaatggagg aacaccacca tgaacgccaa tgaaccgagc
     2041 accagttgct gcgtgtgctg caaggaaatc ccgctcgatg ccgccttcac gccggaaggg
     2101 gccgagtacg tggagcattt ctgcgggctg gagtgctatc agcgcttcca ggcgcgggcc
     2161 agcactgcga ccgaaaccag cgtcaaaccg gacgcttgtg attcgccgcc gtcaggttga
     2221 ggcataccct aacctgatgt cagatgccat gtgtaaattg cgtcaggata ggattgaatt
     2281 ttgaatttat tgacatatct cgttgaaggt catagagtct tccctgacat tttgcaggga
     2341 attccatgac tggacagcgc attgggtata tcagggtcag caccttcgac cagaacccgg
     2401 aacggcaact ggaaggcgtc aaggttgatc gcgcttttag cgacaaggca tccggcaagg
     2461 atgtcaagcg tccgcaactg gaagcgctga taagcttcgc ccgcaccggc gacaccgtgg
     2521 tggtgcatag catggatcgc ctggcgcgca atctcgatga tttgcgccgg atcgtgcaaa
     2581 cgctgacaca acgcggcgtg catatcgaat tcgtcaagga
//
LOCUS       DAMZFT010000292         2608 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.293, whole genome shotgun sequence.
ACCESSION   DAMZFT010000292 DAMZFT010000000
VERSION     DAMZFT010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2608)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2608)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2608
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.293"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(<1..1431)
                     /gene="zwf"
                     /locus_tag="PN977_005255"
     CDS             complement(<1..1431)
                     /gene="zwf"
                     /locus_tag="PN977_005255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HDI6045152.1"
                     /translation="MAVTQTAQACDLVIFGAKGDLARRKLLPSLYQLEKAGQLNPDTR
                     IIGVGRADWDKAAYTKVVREALETFMKETIDEGLWDTLSARLDFCNLDVNDTAAFSRL
                     GAMLDQKNRITINYFAMPPSTFGAICKGLGEAKLNAKPARVVMEKPLGTSLATSQEIN
                     DQVGEYFEECQVYRIDHYLGKETVLNLLALRFANSLFVNNWDNRTIDHVEITVAEEVG
                     IEGRWGYFDKAGQMRDMIQNHLLQILCMIAMSPPSDLSADSIRDEKVKVLKSLRRIDR
                     SNVREKTVRGQYTAGFAQGKKVPGYLEEEGANKSSNTETFVAIRVDIDNWRWAGVPFY
                     LRTGKRLPTKCSEVVVYFKTPELNLFKESWQDLPQNKLTIRLQPDEGVDIQVLNKVPG
                     LDHKHNLQITKLDLSYSETFNQTHLADAYERLLLETMRGIQALFVRRDEVEEAWKWVD
                     SITEAWAMDNDAPKPYQAGTWGPVASV"
     gene            1769..>2608
                     /gene="hexR"
                     /locus_tag="PN977_005256"
                     /gene_synonym="yebK"
     CDS             1769..>2608
                     /gene="hexR"
                     /locus_tag="PN977_005256"
                     /gene_synonym="yebK"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HexR"
                     /protein_id="HDI6045153.1"
                     /translation="MNMLEKIQSQLEHLSKSERKVAEVILASPDNAIHSSIAALALEA
                     NVSEPTVNRFCRSMDTRGFPDFKLHLAQSLANGTPYVNRNVNEDDSVESYTGKIFESA
                     MATLDHVRHSLDKSAINRAVDLLTQAKKIAFFGLGSSAAVAHDAMNKFFRFNVPVVYS
                     DDIVLQRMSCMNCSDGDVVVLISHTGRTKNLVELAQLARENDAMVIALTSAGTPLARE
                     ATLAITLDVPEDTDIYMPMVSRLAQLTVIDVLATGFTLRRGAKFRDNLKRVKEALKES
                     RFDK"
BASE COUNT          635 a          663 c          624 g          686 t
ORIGIN      
        1 cactgaggca acgggtcccc aggttccggc ctgatacggt ttcggcgcat cattgtccat
       61 cgcccacgcc tcagtaatgg agtctaccca tttccaggct tcttccactt catcacgacg
      121 tacaaacagt gcctgaatac cacgcatggt ttccagcagc agacgttcat aggcatctgc
      181 cagatgcgtt tgattaaagg tttctgaata gctcagatcc agcttggtga tttgcaggtt
      241 atgtttatgg tcaaggccag gaactttatt tagtacctgg atatccacgc cttcatcagg
      301 ttgcagacgg atagtcagtt tattctgcgg cagatcctgc cacgactctt taaacagatt
      361 cagttcaggt gttttgaaat agaccacgac ttcagaacat ttggtcggca gacgtttacc
      421 agtacgcagg tagaatggca caccggccca gcgccagtta tcaatgtcga cgcggatcgc
      481 cacgaaggtt tctgtattgc tgctcttgtt cgcgccctct tcttccagat atcccggcac
      541 ttttttgccc tgggcgaagc ccgcagtata ttgcccgcgt acggtttttt cgcgcacgtt
      601 ggagcggtca atgcggcgca gagacttcag tactttgact ttttcatcgc ggatgctgtc
      661 tgcactcagg tcagacggcg gagacatcgc aatcatgcaa agaatttgca gcaggtggtt
      721 ttggatcata tcgcgcatct gaccggcttt atcaaaatag ccccagcgcc cttcgatccc
      781 cacttcttct gccacggtaa tctcaacatg atcaatggtg cgattgtccc agttattcac
      841 aaacagggag ttagcaaaac gcagcgccaa caggttcagc actgtttctt taccaagata
      901 gtggtcgata cggtaaacct ggcactcctc gaagtattcg ccaacctgat cgttgatttc
      961 ctgcgaggtc gccagcgacg tccccagcgg tttctccatg actacgcgtg ccggtttagc
     1021 attcagtttt gcctcaccaa gccctttgca aattgcgcca aaagtgctgg gcggcatggc
     1081 aaagtagtta atggtgatac gatttttttg atccagcatc gcgccgagac ggctgaatgc
     1141 agcagtgtca ttgacatcaa gattacaaaa atccagacgt gcactcaggg tgtcccataa
     1201 accttcatca atcgtttctt tcatgaaagt ttcgagcgcc tcgcggacaa ctttggtata
     1261 agccgcttta tcccagtcag cacgccctac gccgataatc cgggtgtccg ggttgagctg
     1321 accggctttt tccagttgat acagggaagg cagcaattta cgacgcgcaa ggtcgccttt
     1381 cgcgccgaaa atgaccaggt cacaggcctg ggctgtttgc gttaccgcca tgtcattctc
     1441 cttaagttaa ctaacccggt acttaagcca gggtatactt gtaattttct tacggtgcac
     1501 tgtactgctt ttacgagctt gcgaaaactg taaacgctta tccacccgtg cgattacggt
     1561 aaaagcgcgc aaagtgcggc aaaactgata aaaaaatcat cgttttccat tgggtaaaaa
     1621 tctgacactg atcatgttat gaaaaaaaat aacaactttt ttatctgctt ttgtcattaa
     1681 cacagcacac aggcgtaata tctggcaaaa ctgcatttcg atttctttca gtgcggaaat
     1741 cgtcattacc cgtgagtctc tttacatcat gaatatgctg gaaaaaatcc agtctcagct
     1801 ggaacatttg agcaaatcag agcgcaaagt tgccgaggtc attctggctt cgcccgataa
     1861 cgcgatccat tcgagtattg ctgctctggc gctggaagcc aatgttagcg aaccgacggt
     1921 gaatcgtttc tgtcgcagca tggacacgcg cggttttcct gattttaaac ttcatctggc
     1981 acaaagtctg gcgaatggca ctccctatgt taatcgcaac gtcaatgaag atgacagcgt
     2041 tgaatcatac acggggaaaa tatttgagtc cgcaatggca acgctcgatc atgtccgtca
     2101 ttcactggat aaatctgcca tcaaccgcgc cgtcgacttg ctcactcagg caaaaaaaat
     2161 cgcctttttc ggattaggct cttcagccgc cgttgcccac gatgcgatga ataagttctt
     2221 tcgttttaat gttccggtgg tgtactccga tgatattgtg ctgcaacgca tgagttgtat
     2281 gaattgtagc gacggagacg tggtggtgct gatttctcac acgggaagaa caaaaaatct
     2341 ggtcgagctg gcgcagctgg cacgcgaaaa cgacgccatg gtgattgccc tcacctctgc
     2401 gggtaccccg ctcgcccggg aagcaacgct ggctattacc ctcgacgtac cggaagatac
     2461 tgacatttat atgcccatgg tttctcgact tgcacagctg accgtgatag atgtgctggc
     2521 gacaggattt actttgcggc gcggtgcaaa attcagagat aacttgaagc gggtcaagga
     2581 agcgctgaag gaatcgcgtt ttgataag
//
LOCUS       DAMZFT010000293         2576 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.295, whole genome shotgun sequence.
ACCESSION   DAMZFT010000293 DAMZFT010000000
VERSION     DAMZFT010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2576)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2576)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2576
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.295"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..1288
                     /gene="norR"
                     /locus_tag="PN977_005257"
     CDS             <1..1288
                     /gene="norR"
                     /locus_tag="PN977_005257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="nitric oxide reductase transcriptional regulator
                     NorR"
                     /protein_id="HDI6045154.1"
                     /translation="EAIARAGDVVRFPADSELPDPYDGLIPGQESLKVHACVGLPLFA
                     GQNLIGALTLDGMQPDQFDVFSDEELRLIAALAAGALSNALLIEQLESQNMLPGDAAP
                     FEAVKQTQMIGLSPGMTQLKKEIEIVAASDLNVLISGETGTGKELVAKAIHEASPRAV
                     NPLVYLNCAALPESVAESELFGHVKGAFTGAISNRSGKFEMADNGTLFLDEIGELSLA
                     LQAKLLRVLQYGDIQRVGDDRSLRVDVRVLAATNRDLREEVLAGRFRADLFHRLSVFP
                     LSVPPLRERGDDVILLAGYFCEQCRLRLGLSRVVLSARARNLLQHYNFPGNVRELEHA
                     IHRAVVLARATRSGDEVILEAQHFAFPEVTLPPPEAAAVPIVKQNLREATEAFQRETI
                     RQALAQNHHNWAASARMLETDVANLHRLAKRLGLKD"
     gene            complement(1285..2250)
                     /gene="gutQ"
                     /locus_tag="PN977_005258"
     CDS             complement(1285..2250)
                     /gene="gutQ"
                     /locus_tag="PN977_005258"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase GutQ"
                     /protein_id="HDI6045155.1"
                     /translation="MSEALLNAGRQTLMLELQEASRLPERLGDDFVRAANIILHCEGK
                     VVVSGIGKSGHIGKKIAATLASTGTPAFFVHPAEALHGDLGMIESRDVMLFISYSGGA
                     KELDLIIPRLEDKSIALLAMTGKPTSPLGLAAKAVLDISVEREACPMHLAPTSSTVNT
                     LMMGDALAMAVMQARGFNEEDFARSHPAGALGARLLNKVHHLMRRDDAIPQVALTASV
                     MDAMLELSRTGLGLVAVCDAQQQVQGVFTDGDLRRWLVGGGALTTPVNEAMTVGGTTL
                     QSQSRAIDAKEILMKRKITAAPVVDENGKLTGAINLQDFYQAGII"
     gene            complement(2243..>2576)
                     /gene="srlR"
                     /locus_tag="PN977_005259"
                     /gene_synonym="gutR"
     CDS             complement(2243..>2576)
                     /gene="srlR"
                     /locus_tag="PN977_005259"
                     /gene_synonym="gutR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glucitol operon DNA-binding transcriptional
                     repressor SrlR"
                     /protein_id="HDI6045156.1"
                     /translation="KSASFHGQLAENAFEHFTFDKLFMGTDGIDLNAGVTTFNEVYTV
                     SKAMCNAAREVILMADSSKFGRKSPNVVCSLESVDKLITDVGIDPAFRQALEKKGIDV
                     IITGESNE"
BASE COUNT          543 a          694 c          758 g          581 t
ORIGIN      
        1 ggaagcgatt gcccgcgccg gggacgtggt gcgctttccc gcagacagcg aattgcccga
       61 tccctatgac ggtttgattc ccgggcagga gagtctgaag gttcacgcct gcgttggtct
      121 gccattgttt gccgggcaaa acctgatcgg cgcattgacg ctcgacggga tgcagcccga
      181 tcagttcgat gttttcagcg acgaagagtt acgcctgatt gccgcgctgg cggcgggagc
      241 gttaagcaat gcgttgctga ttgagcaact ggaaagccag aatatgctgc cgggcgatgc
      301 cgcgccgttt gaagcggtga aacagacgca gatgatcggt ctgtcgccag gcatgacgca
      361 attgaaaaaa gagattgaga ttgtggcggc gtccgatctc aacgtcttaa tcagcggtga
      421 gacgggaacc ggtaaggagc tggtggcgaa agcgattcat gaagcctcgc caagggcggt
      481 gaatccgctg gtctatctca actgtgccgc actgccggaa agtgtggcgg aaagtgagtt
      541 gttcgggcac gtgaaagggg cgtttactgg cgctatcagt aaccgcagcg ggaagtttga
      601 aatggcggat aacggcacgc tgtttctgga tgagatcggc gagttgtcgt tggcattgca
      661 ggccaagctg ctgcgggtgt tgcagtatgg cgatattcag cgcgttggcg atgaccgcag
      721 tttgcgggtc gatgtgcgcg tgctggcggc gactaaccgt gacttacgcg aagaggtact
      781 ggcagggcga tttcgcgctg acttgttcca ccgcctgagc gtgtttccac tttcagtgcc
      841 gccgctgcgt gagcggggcg atgatgtcat tctgctggcg gggtatttct gcgaacagtg
      901 ccgcctgcgg ttggggctct cccgcgtggt attaagtgcc agagcgcgaa atttactgca
      961 acactacaat tttccgggga acgttcgtga actggaacat gctattcatc gggcggtagt
     1021 gctggcgaga gccactcgca gcggcgatga agtgattctt gaagcgcaac attttgcttt
     1081 tcctgaggtg acgttgccgc cgccagaagc ggcggcggtg cctatcgtta agcaaaacct
     1141 gcgtgaagcg acagaagcgt tccagcgtga aaccattcgt caggcactgg cacaaaatca
     1201 tcacaactgg gctgccagcg cacggatgct ggaaaccgac gtcgccaact tgcatcggtt
     1261 ggcgaaacgt ctggggctga aggattaaat aatcccggcc tgatagaaat cctgcaggtt
     1321 aattgcgccg gtgagtttgc cgttttcatc cactaccggt gcggcggtga ttttgcgttt
     1381 catcaggatt tctttggcgt cgatggcacg gctttgcgat tgcaacgtgg tgccgccgac
     1441 cgtcatcgct tcattgactg gcgtggtgag tgcgccgccg ccaaccagcc agcgacgtaa
     1501 atcaccgtcg gtaaagacgc cttgtacctg ttgctgagcg tcacataccg ccaccagccc
     1561 cagaccggtg cggctgagtt ccagcatcgc atccatcacg ctggcggtta acgctacctg
     1621 cggaatagcg tcatcacggc gcatcagatg atgcacttta ttcagcaagc gagcgcccag
     1681 tgccccggct gggtgggagc gggcaaaatc ttcttcatta aaaccacgcg cctgcatgac
     1741 tgccatcgcc aacgcgtcgc ccatcatcag ggtattcacg gtgctggagg tcggcgcaag
     1801 atgcatcggg caggcttcgc gttctacgga gatatccagc accgctttcg ccgccaggcc
     1861 cagcggtgac gtcggtttgc cggtcatcgc cagcagcgcg atagatttat cttccagacg
     1921 cggaataatc agatccagtt ccttcgcgcc accggagtaa gagataaaca gcatcacatc
     1981 gcggctttcg atcatcccca ggtcgccgtg caatgcctct gccggatgga caaaaaaggc
     2041 aggagtgccg gtgctggcaa gcgttgcggc gattttctta ccaatgtggc ccgatttgcc
     2101 aattcccgaa accaccactt tgccttcgca gtgcaggatg atattggcgg cgcgaacaaa
     2161 atcatcgccc agacgttccg gtaaacggct tgcttcctgt aactccagca ttaacgtctg
     2221 acgtcccgcg ttcagtagtg cttcactcat tgctctctcc ggttatgatc acatcgatcc
     2281 ctttcttttc cagcgcctga cgaaacgccg gatcgatacc tacgtcggta attagcttat
     2341 cgacgctttc aagactacaa accacgttgg ggcttttacg accaaacttc gatgagtcag
     2401 ccatcaaaat cacttcgcgc gcggcattac acatcgcctt actgacggta tagacctcgt
     2461 tgaaggtggt tacgcccgca ttgagatcga tgccgtcggt gcccataaac aatttatcga
     2521 aggtgaaatg ctcgaaggca ttctctgcca gttgcccgtg aaatgaggcc gatttt
//
LOCUS       DAMZFT010000294         2567 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.296, whole genome shotgun sequence.
ACCESSION   DAMZFT010000294 DAMZFT010000000
VERSION     DAMZFT010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2567)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2567)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2567
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.296"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(<1..203)
                     /gene="tuf"
                     /locus_tag="PN977_005260"
     CDS             complement(<1..203)
                     /gene="tuf"
                     /locus_tag="PN977_005260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HDI6045157.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHV"
     gene            complement(318..393)
                     /locus_tag="PN977_005261"
     tRNA            complement(318..393)
                     /locus_tag="PN977_005261"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(358..360),aa:Thr,seq:ggt)
     gene            complement(400..474)
                     /locus_tag="PN977_005262"
     tRNA            complement(400..474)
                     /locus_tag="PN977_005262"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(439..441),aa:Gly,seq:tcc)
     gene            complement(591..675)
                     /locus_tag="PN977_005263"
     tRNA            complement(591..675)
                     /locus_tag="PN977_005263"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(639..641),aa:Tyr,seq:gta)
     gene            complement(684..759)
                     /locus_tag="PN977_005264"
     tRNA            complement(684..759)
                     /locus_tag="PN977_005264"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(724..726),aa:Thr,seq:tgt)
     gene            1121..2071
                     /gene="coaA"
                     /locus_tag="PN977_005265"
                     /gene_synonym="panK"
     CDS             1121..2071
                     /gene="coaA"
                     /locus_tag="PN977_005265"
                     /gene_synonym="panK"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HDI6045158.1"
                     /translation="MSIKEQTLMTPYLQFDRNQWAALRDSVPMTLSEDEIARLKGINE
                     DLSLEEVAEIYLPLSRLLNFYISSNLRRQAVLEQFLGTNGQRIPYIISIAGSVAVGKS
                     TTARVLQALLSRWPEHRRVELITTDGFLHPNQVLKERGLMKKKGFPESYDMHRLVKFV
                     SDLKSGVPNVTAPVYSHLIYDVIPDGDKTVVQPDILILEGLNVLQSGMDYPHDPHHVF
                     VSDFVDFSIYVDAPEDLLQTWYINRFLKFREGAFTDPDSYFHNYAKLTKEEAINTAMT
                     LWKEINWLNLKQNILPTRERASLILTKSANHAVEEVRLRK"
     gene            complement(2100..>2567)
                     /locus_tag="PN977_005266"
     CDS             complement(2100..>2567)
                     /locus_tag="PN977_005266"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HDI6045159.1"
                     /translation="DKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMA
                     MRRVEESVVNQGWITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKL
                     DNFINRPVKLIIGDKEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
BASE COUNT          670 a          614 c          581 g          702 t
ORIGIN      
        1 acgtgagaag tgttgatggt gataccacga gctttttctt ccggcgcgtt atcgatctgg
       61 tcgaatgcgc gagcagcacc gccgtaggtt ttagccagta cggtagtgat tgcagcggtc
      121 agcgttgttt taccatggtc aacgtggccg atagtaccga cgttaacgtg cggttttgta
      181 cgttcaaact tttctttaga catcgattgt ccctctaaga cacggataaa tcggtgatat
      241 caccacatca accaggcaac atgcccgact tgttgaatgc aataaacaga aggaaaaaac
      301 agggaggaga aaagaagtgg tgctgatagg cagattcgaa ctgccgacct cacccttacc
      361 aagggtgcgc tctaccaact gagctatatc agcacatctt ggagcgggca gcgggaatcg
      421 aacccgcatc atcagcttgg aaggctgagg taatagccat tatacgatgc ccgcatcctg
      481 gaactcggct acctgatttt cattctgcac tgaatatcga gagaagctct ctttattcga
      541 gccggtaagc gaacttatcg tctcgggcta cgccatcgcg tggccgaaat tggtggtggg
      601 ggaaggattc gaaccttcga agtctgtgac ggcagattta cagtctgctc cctttggccg
      661 ctcgggaacc ccaccggact tgatggtgcc gactaccgga atcgaactgg tgacctactg
      721 attacaagtc agttgctcta cctactgagc taagtcggca tcaagtagcg cgcattctat
      781 ggagacatgc gagttcatgc aactaaaaaa ttgcataatt tgttttattg gtcacatttt
      841 atgcgacacg atgaagaaac agccgaacgg agggcagatt agcacacttt ttcaacatca
      901 ttgtgctcaa caatgcgctc ctgctaaacc ataattcttt ttatcagatg gaatatctgt
      961 cacattgctt ttcaacgata gcttcctggg agagattttt tcttattatt cctccccatc
     1021 tggtgttacc ctcctgccca ttaacccatt caacagaact gtgacgcgcc atggcaaata
     1081 tcgctttgcc gatagagcta tgaccgccag aaacatgctt atgagtataa aagagcaaac
     1141 gttaatgacg ccttacctac agtttgaccg caaccagtgg gcagctctgc gtgattccgt
     1201 acctatgacg ttatcggaag atgagatcgc ccgtctcaaa ggtattaatg aagatctctc
     1261 gttagaagaa gttgccgaga tctatttacc tctgtcacgt ttgctgaact tctatataag
     1321 ctcgaatctg cgccgtcagg cagttctgga acagtttctt ggtaccaacg ggcaacgcat
     1381 tccttacatt atcagtattg ctggtagtgt cgcggtgggg aaaagtacaa ccgcccgtgt
     1441 attgcaggcg ctattaagcc gttggccgga acatcgtcgt gttgaactga tcacgactga
     1501 tggcttcctt caccctaatc aggtactgaa agaacgtggt ctgatgaaga agaaaggctt
     1561 cccggaatcg tatgatatgc atcgcctggt gaagtttgtt tccgatctca aatccggcgt
     1621 gccaaacgtt acagcacctg tttactcgca tcttatttat gatgtgatcc cggatggaga
     1681 taaaacggtt gttcagcctg atattttaat tcttgaaggg ttaaatgtct tacagagcgg
     1741 aatggattat ccacacgatc cacatcatgt atttgtttct gattttgtcg atttttcgat
     1801 atatgttgat gcaccggaag acttacttca gacgtggtat atcaaccgtt ttctgaaatt
     1861 ccgggaaggg gcttttacag acccggattc ctattttcat aactacgcga aattaactaa
     1921 agaagaagcg attaatactg ccatgacgtt gtggaaagag atcaactggc tgaacttaaa
     1981 gcaaaatatt ctacctactc gcgagcgcgc cagtttaatc ctgacgaaaa gtgctaatca
     2041 tgcggtagaa gaggtcagac tacgcaaata attttcaggg gagctaatgc tcccctatct
     2101 tatttttccg cactacgcag ggatatttca ccgcccatcc agggttttat tattccatcc
     2161 tgctcaagca ataaagcgcc ctgtttgtct attccacgtg aaatgccaaa tatttcttta
     2221 tcaccaatga taagtttcac tgggcgatta ataaaattat ccagcttttc ccagcgcgaa
     2281 agataaggtg ccaatccttc ttgctcgaag agttccagcg ccgcgcgtaa ttcacgtatg
     2341 agcatggccg ccaacgtatt acgatcgaga ttgattcccg cttcctgcag cgtgatccac
     2401 ccttgattaa cgacactctc ttcaacacga cgcattgcca tgttgatccc ggctccaatg
     2461 actatttgcg ccgcatcgcc ggttttgcca gttagctcca caagaatgcc tgccagcttg
     2521 cgatcctgca aatagaggtc attaggccat ttaacacgaa ctttatc
//
LOCUS       DAMZFT010000295         2481 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.297, whole genome shotgun sequence.
ACCESSION   DAMZFT010000295 DAMZFT010000000
VERSION     DAMZFT010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2481)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2481)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2481
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.297"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(<1..271)
                     /gene="chbA"
                     /locus_tag="PN977_005267"
     CDS             complement(<1..271)
                     /gene="chbA"
                     /locus_tag="PN977_005267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIA"
                     /protein_id="HDI6045160.1"
                     /translation="MMDLDNIPDTQTEAEELEEVVMGLIINSGQARSLAYAALKQAKQ
                     GDFAAAKAMMDQSRMALNEAHLVQTKLIEGDAGEGKMKVSLVLVHA"
     gene            complement(322..1680)
                     /gene="chbC"
                     /locus_tag="PN977_005268"
     CDS             complement(322..1680)
                     /gene="chbC"
                     /locus_tag="PN977_005268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIC"
                     /protein_id="HDI6045161.1"
                     /translation="MSNVIASLEKVLLPFAVKIGKQPHVNAIKNGFIRLMPLTLAGAM
                     FVLINNVFLSFGEGSFFYSLGIRLDASTIETLNGLKGIGGNVYNGTLGIMSLMAPFFI
                     GMALAEERKVDALAAGLLSVAAFMTVTPYSVGEAYAVGANWLGGANIISGIIIGLVVA
                     EMFTFIVRRNWVIKLPDSVPASVSRSFSALIPGFIILSVMGIIAWALNTWGTNFHQII
                     MDTISTPLASLGSVVGWAYVIFVPLLWFFGIHGALALTALDNGIMTPWALENIATYQQ
                     YGSVEAALAAGKTFHIWAKPMLDSFIFLGGSGATLGLILAIFIASRRADYRQVAKLAL
                     PSGIFQINEPILFGLPIIMNPVMFIPFVLVQPILAAITLAAYYMGIIPPVTNIAPWTM
                     PTGLGAFFNTNGSVAALLVALFNLGIATLIYLPFVVVANKAQNAIDKEESEEDIANAL
                     KF"
     gene            complement(1765..2085)
                     /gene="chbB"
                     /locus_tag="PN977_005269"
     CDS             complement(1765..2085)
                     /gene="chbB"
                     /locus_tag="PN977_005269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIB"
                     /protein_id="HDI6045162.1"
                     /translation="MEKKHIYLFCSAGMSTSLLVSKMRAQAEKYEVPVIIEAFPETLA
                     GEKGQNADVVLLGPQIAYMLPEIQRLLPNKPVEVIDSLLYGKVDGLGVLKAAVAAIKK
                     AAAN"
     gene            complement(2384..>2481)
                     /locus_tag="PN977_005270"
     CDS             complement(2384..>2481)
                     /locus_tag="PN977_005270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310472.1"
                     /note="osmotically-inducible lipoprotein E; activator of
                     ntr-like gene protein; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDI6045163.1"
                     /translation="TYFVALDDTGHVINSGYQTCAEYDTDPQAAK"
BASE COUNT          693 a          629 c          545 g          614 t
ORIGIN      
        1 gagcgtggac cagcaccaga ctcactttca tcttaccttc gcccgcatcg ccttcaatca
       61 gtttcgtctg taccagatgc gcttcattca atgccatccg tgactgatcc atcatggctt
      121 ttgctgcggc aaaatcgccc tgcttcgcct gtttcagtgc cgcatacgcc aggctgcgcg
      181 cttgtccgga gttgatgata agccccatca ccacttcttc cagctcttca gcttctgttt
      241 gcgtatcggg aatgttatcg agatccatca tacatcgttc ctctcttttc ttaccggcac
      301 gattacccgt accggcatcg attaaaattt cagggcgtta gcgatatctt cttcgctctc
      361 ttctttatca atcgcgtttt gtgctttgtt agcgaccaca acaaagggca gataaattaa
      421 cgttgcgata ccaaggttga agagtgcgac cagcaatgcg gcgacgctac cgttggtgtt
      481 aaagaaggct cccagaccgg ttggcatggt ccacggtgca atattggtca ccggaggaat
      541 aatgcccatg tagtacgctg cgagggtgat tgccgccaga atcggttgta ccagtacaaa
      601 cgggataaac atcaccgggt tcatgataat tggcagacca aacagaatcg gttcgttaat
      661 ctggaagatg ccggacggca gcgccagttt tgccacctga cgataatcag cacggcgaga
      721 ggcgataaag atagccagga tcagacctaa agtcgcacca ctgcccccaa ggaaaataaa
      781 ggagtccagc atcggcttgg cccagatatg gaaggtctta ccggctgcca gcgccgcttc
      841 gacggaacca tattgctgat aggtcgcgat attttccagc gcccaaggcg tcataatgcc
      901 gttgtccagt gcggtcagcg ccagcgcacc atgaataccg aagaaccaga gcagtggaac
      961 aaagatcaca taggcccagc ccaccacact acccaacgat gccagtgggg ttgagatggt
     1021 atccataatg atctgatgga agttggtgcc ccaggtattc aacgcccagg caataatccc
     1081 catcacggaa agaataataa agccgggaat taaagccgag aaggaacgcg atactgaagc
     1141 aggtacgcta tcgggcaatt taatgaccca attgcggcgg acaataaagg taaacatttc
     1201 tgccaccacc aggccaataa taatcccgga gatgatattc gccccaccta accagtttgc
     1261 gccaaccgca taggcctcac cgacactata tggggtgacg gtcataaatg ctgcaacgga
     1321 taacaacccg gccgccagcg catcgacttt acgctcttct gccagcgcca tgccaataaa
     1381 gaacggtgcc attaaagaca ttatccctaa tgttccgtta tatacgttgc cgccaatacc
     1441 tttcagacca ttaagtgttt caatggttga ggcatccagg cgaataccta aggaataaaa
     1501 aaacgacccc tccccaaagc ttagaaaaac gttgttaatt aatacaaaca tggcccccgc
     1561 aagggttaac ggcattaagc gaataaagcc atttttgatt gcattaacgt gtggctgctt
     1621 tcctatttta actgcaaaag ggaggagtac cttttcaagc gatgcaataa cattactcat
     1681 agaaaaatac ccttaaaaac cgcaatttaa atattgcggt attgatttat gaaataactc
     1741 tttgacggga aaatttaaaa taaattaatt tgctgcggct tttttaatcg ctgcaaccgc
     1801 agccttaagc acgcctaaac catcgacttt gccataaagc agcgagtcaa ttacttcaac
     1861 cggtttgttg ggtaacaaac gctggatttc gggcaacata taagcaatct gcggccctaa
     1921 taacacgaca tcggcattct gacctttttc accagccagt gtttccggaa atgcttcaat
     1981 aatgaccgga acttcatatt tttctgcctg tgcgcgcatt tttgatacca gtaaagaggt
     2041 agacatgccc gcagaacaaa acagataaat gtgtttcttt tccataaaac tgccctcatc
     2101 gacgattatc tgtaagccag acactccgca agccttaacc tgcttccatg ctctgggtaa
     2161 cttgcgaaac caacatgact ttttttgtgg ctgaaaggag tatacggtat agacctgagc
     2221 aatagtattt ctttgacctt ataaattgtc tctccttgac ctgcggttat gaccctcttc
     2281 acatttcggg caaataattc gcgaagataa ttaagaactc aggccaaaaa aaaccggcac
     2341 aatggccggt ttccgttgta actcaagctt tcaggcgaag tgattacttc gcagcctgtg
     2401 gatcagtgtc gtattcagca caggtctgat aaccggagtt gatgacatgt ccggtatcat
     2461 ctaacgcgac aaagtaggtt t
//
LOCUS       DAMZFT010000296         2440 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.298, whole genome shotgun sequence.
ACCESSION   DAMZFT010000296 DAMZFT010000000
VERSION     DAMZFT010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2440)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2440)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2440
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.298"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            100..1641
                     /gene="istA"
                     /locus_tag="PN977_005271"
     CDS             100..1641
                     /gene="istA"
                     /locus_tag="PN977_005271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001298859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element ISEc12 family transposase"
                     /protein_id="HDI6045164.1"
                     /translation="MPTVPISMRKLKEILRLKYGVGLSHRQIGRSLAISPSVVSRYAN
                     RAAQLGIKQWPLPTGWDDTKLKHAFLQTQVKMKKHSLPDWATVHRELRNKCVTLQLLW
                     EEYCERNPGGFYSYNHYCRMYREWLKTTSPSMRQVHKAGEKLFVDYCGPTVGVTDPET
                     GEIRTAQVIVAVLGASSYTWAEATWSQQLEDWVMSHVRCFQWLGGVPELVVPDNLKSA
                     TSRACKYDPDVNPTYQQMLEHYNVAVLPARPRKPKDKAKAEVGVQVVERWIMARIRHE
                     IFYSLASLNQRIRELLERLNNKIMQKLGYSRAELFIQLDKPALKPLPEASYSYTLVKK
                     VRVHADYHVEIDKHYYSVPCSLLGQQLEAWISGELVRLFNQGQEVAVHPRKRTYGYST
                     RNEHMPEAHRQHATWTPERLLEWAGHIGSETHSYVLHILNSRPHPEQSYRFCLGLLNL
                     HKKYSKARLNAACARALKTKVWRLSGIKSILEKGLDKQPVQDPKPDLLSTMEHENVRG
                     SEYYH"
     gene            1656..2402
                     /gene="istB"
                     /locus_tag="PN977_005272"
     CDS             1656..2402
                     /gene="istB"
                     /locus_tag="PN977_005272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001514323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element ISEc12 family helper ATPase
                     IstB"
                     /protein_id="HDI6045165.1"
                     /translation="MNHLYEQLTALKLTGFRDALKKQLAQPGTYQELGFEERLSLLTA
                     EELTCRENRKAERLIKHARFRLNAELSKLDYRNNRGLDRALIRSLSQGNWLTLKQNIL
                     LTGATGSGKTFLACALGHNACRQGYKVYYYRLKALMEQCYQGHADGRYSKLLTRLNNS
                     DLLLLDDWGLEPLSSEQRSDLLEIVDLMYQRGSIIVVSQLPVENWYKMIGDSTHADAI
                     LDRLVHGSIKIELKGESMRKIQSPLTEGDQ"
BASE COUNT          694 a          574 c          590 g          582 t
ORIGIN      
        1 cttttgactc attttgttat tcagtgatct ccatcaatgt tattggaact tcacaggtgt
       61 gttgatctgt atcttttgcc attccggtaa aggataccta tgccaacagt tccaatttct
      121 atgagaaaac ttaaagaaat tcttaggctt aaatacggtg ttggactcag ccatcgacaa
      181 attggtcgta gtcttgcaat ctccccttcc gttgtatcca gatatgctaa tcgggcggct
      241 caacttggca taaagcagtg gcccttacct acaggatggg atgatacaaa actaaaacat
      301 gcgttccttc agacccaggt taagatgaag aagcactctc tgcctgactg ggctacagta
      361 caccgggaac tgcgtaataa atgcgtgacg ctgcagctac tctgggaaga atactgtgag
      421 cgtaatccag gcggttttta cagctataac cattactgcc ggatgtaccg tgaatggctc
      481 aaaaccactt caccatcaat gcgtcaggta cataaagctg gcgaaaaact tttcgttgat
      541 tactgtggac ctaccgttgg cgttaccgac cctgagaccg gagaaataag aactgctcag
      601 gtcatcgtag ctgttctcgg ggcatcaagt tacacatggg cagaggccac ctggtctcag
      661 cagcttgaag actgggtgat gagtcatgtt cgctgcttcc agtggttggg tggcgttcct
      721 gaacttgttg ttccggacaa tctgaaaagc gccacatcca gggcatgtaa gtatgatcct
      781 gacgttaacc ctacctacca gcagatgctt gagcattata atgtcgcagt tttgcctgcg
      841 cggccacgta aaccgaaaga taaagccaaa gctgaagttg gcgttcaggt tgttgaacgc
      901 tggatcatgg cccgaatcag gcatgagatc ttctacagcc ttgcatcgct taatcagcgc
      961 attcgggagt tgctggaaag actgaataac aaaataatgc agaagttggg ttattcacgt
     1021 gcagaactct tcatccagct tgataaaccc gcactgaagc ctcttcctga agccagttac
     1081 agttacaccc tggtgaagaa agtcagagtt catgccgatt accacgtgga aatcgacaaa
     1141 cattactact cggttccatg ttcgctgtta ggccagcaac tggaagcatg gatctccgga
     1201 gaactggtaa gactcttcaa tcaggggcag gaggttgctg tgcacccgcg caagcgtact
     1261 tatggctaca gtacccgcaa cgagcacatg cctgaagctc atcgacagca tgccacctgg
     1321 acgccagagc gtcttctgga atgggcgggg cacataggca gtgaaactca tagttatgtg
     1381 cttcatatac tgaactctcg tccacatccg gaacaaagct atcgcttctg ccttggactc
     1441 ctgaaccttc ataaaaaata cagtaaagcc agacttaatg cagcatgtgc aagagctctg
     1501 aaaacaaagg tatggcgtct gtcaggtatt aaatcgatcc tggaaaaagg tctggataaa
     1561 caacctgttc aggatccaaa accagatctg ttatccacga tggaacacga aaacgtacgc
     1621 ggcagtgagt attaccactg atacgggatc caatgatgaa tcatctttac gaacaactga
     1681 ccgcacttaa actcaccggc ttccgtgatg cgcttaaaaa gcaacttgct cagccgggca
     1741 cataccagga gctgggcttc gaagaacgcc tgtcattact gacagcagaa gaactaacct
     1801 gccgtgaaaa caggaaggca gagcgtctga tcaaacatgc acggttcaga cttaatgctg
     1861 agttatcaaa gctggattat cgtaacaata gagggctgga cagggccctc atccgttcac
     1921 tcagtcaggg aaactggtta accctgaaac aaaatatttt actgaccggg gccaccggca
     1981 gcggtaaaac gttcctggca tgtgcacttg gtcataatgc ctgccgacag ggatacaagg
     2041 tctactatta tcgccttaaa gcgctgatgg aacagtgcta tcaggggcat gctgatggaa
     2101 gatacagcaa acttttgacc aggctgaata atagcgatct gctgcttctg gatgactggg
     2161 ggctggaacc tctctcatca gaacagcgta gcgacctgct ggaaatagtg gatctgatgt
     2221 accaacgagg ctcaatcatc gtagtgagcc agttgccggt ggaaaactgg tacaaaatga
     2281 tcggagactc cacacatgcg gatgccatcc tagatcgact ggttcatggc agtatcaaga
     2341 tcgaacttaa aggagaatca atgcggaaaa tacaatctcc gttgaccgaa ggagatcagt
     2401 gaaggtaatt taaaaacggt tcagtgaaag tgacacgaac
//
LOCUS       DAMZFT010000297         2342 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.299, whole genome shotgun sequence.
ACCESSION   DAMZFT010000297 DAMZFT010000000
VERSION     DAMZFT010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2342)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2342)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2342
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.299"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(142..225)
                     /gene="yohP"
                     /locus_tag="PN977_005273"
     CDS             complement(142..225)
                     /gene="yohP"
                     /locus_tag="PN977_005273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YohP"
                     /protein_id="HDI6045166.1"
                     /translation="MKIILWAVLIIFLIGLLVVTGVFKMIF"
     gene            449..1885
                     /gene="mdtQ"
                     /locus_tag="PN977_005274"
     CDS             449..1885
                     /gene="mdtQ"
                     /locus_tag="PN977_005274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001583680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug resistance outer membrane protein
                     MdtQ"
                     /protein_id="HDI6045167.1"
                     /translation="MNRDSFYPAIACFPLLLMLAGCAPMHETRQELSQQTPAAQVDTA
                     LPTALKNGWPDSQWWLEYHDNQLTSLINNALQNAPDMQVAEQRIQLAEAQAKAVATQD
                     GPQLDFSADMERQKMSAEGLMGPFALNDPAAGTTGPWYTNGTFGLTAGWHLDIWGKNR
                     AEVTARLGTVKARVAEREQTRQLLAGSVARLYWEWQTQAALNTVLQQIEKEQNTLIVT
                     DRQLYQNGITSSVEGVETDINASKTRQQLNDVAGKMKIIEARLSALTNHQTKSLKLKP
                     VALPKVASQLPDELGYSLLARRADLQAAHWYVESSLSTIDAAKAAFYPDINLMAFLQQ
                     DALHLSDLFRHSAQQMGVTAGLTLPIFDSGRLNANLDIAKAESNLSIASYNKAVVEAV
                     NDVARAASQVQTLAEKNQHQAQIERDALRVVGLAQARFNAGIIAGSRVSEARIPALRE
                     RANGLLLQGQWLDASIQLTGALGGGYKR"
     gene            1938..>2342
                     /locus_tag="PN977_005275"
     CDS             1938..>2342
                     /locus_tag="PN977_005275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HDI6045168.1"
                     /translation="MAQVAIITASDSGIGKECALLLAQQGFDIGITWHSDEEGAKDTA
                     REVVSHGVRAEIVQLDLGKLPEGALALAKLIQRLGRIDVLVNNAGAMTKAPFLDMAFD
                     EWRKIFTVDVDGAFLCSQIAARQMVKQGQGGRI"
BASE COUNT          588 a          563 c          647 g          544 t
ORIGIN      
        1 gcagggggcg gcggcgattg gccgccccct gcgcgctcct tgcgccagtg atgatatgaa
       61 gcaatgcaca tgacaggagc gcaacaggat gagagactga aatattctcg ttattttcgg
      121 atctgatgac gttaattaat tttaaaatat catcttaaat acgccagtca ccaccagtag
      181 cccaatcagg aaaataatca atacagccca gagtataatt ttcattggcg ttcctttatt
      241 tgtgagtgct gaataagtat aggaaaaaac gaagcccttt gccgttatcc ataaacgatt
      301 aagataaatc ggaagatatc attcacttag tgtatatttt tctggatatg tcgttttatt
      361 gctactgatg tattgccacg cgaaactaaa aaatatatcg caacacacgg taatctccgc
      421 gcaccttttc ttacctgatt attcccccat gaatcgtgat tctttttatc cagccatcgc
      481 ctgttttccg ctgttactga tgctggccgg gtgtgcgcct atgcatgaaa cccgccagga
      541 gttaagccag caaacacccg cggcacaagt tgacaccgca ttacccacgg cgctgaaaaa
      601 tggttggcca gacagccaat ggtggctgga gtatcacgat aatcaactca cttcattaat
      661 taacaatgca ctacagaacg caccggatat gcaggtggct gaacagcgca ttcagctggc
      721 tgaagcccag gcgaaggcag ttgccactca ggatggtccg cagctcgact tttctgcgga
      781 tatggagcgg caaaaaatgt cggcagaagg gttaatgggg ccgtttgctt tgaacgatcc
      841 ggccgcaggt acgaccggtc cgtggtacac caacggtact tttggcttaa cggcgggctg
      901 gcatctcgat atctggggaa agaatcgggc ggaggtaact gctcgcctgg gtacggttaa
      961 agcacgggtg gcggaacgcg agcaaacccg ccaattgctg gctggcagcg tagcccgcct
     1021 gtactgggag tggcaaaccc aggcggcgtt aaacacggtc ttgcagcaaa tcgaaaaaga
     1081 gcagaacacc cttatcgtga ccgatcgcca gctatatcag aacgggatta cttcttcagt
     1141 tgaaggtgtg gaaaccgata ttaatgccag caaaacccgg cagcagctca acgatgtcgc
     1201 ggggaaaatg aaaattattg aggcacggtt aagtgcgctc accaaccacc agacaaagtc
     1261 attgaagctt aaaccggtcg cgttgccgaa agtggcaagt cagcttcctg atgaactcgg
     1321 gtactcctta ctggcccggc gggcagattt gcaggcggcg cactggtacg ttgagtcatc
     1381 gttaagcact attgatgcgg caaaagcggc attttatcct gacatcaacc tgatggcctt
     1441 tctgcaacag gatgcgttgc acttaagcga tctgttccgt cattccgcgc agcaaatggg
     1501 cgttacggca ggcctgacgc tacccatttt cgatagtggt cgtcttaacg ccaatctcga
     1561 tatcgcaaaa gccgaaagca acttgtctat cgccagctac aacaaagcgg tggttgaagc
     1621 ggtgaatgac gtggcgcggg cagccagtca ggttcagaca ctggcggaga aaaaccagca
     1681 tcaggcgcaa attgagcgcg atgccttacg tgtggtaggt cttgcgcagg cccgctttaa
     1741 cgcgggtatc attgccggtt cccgcgtcag cgaagccagg atcccggcgc tgcgtgagcg
     1801 ggccaatggc ctgttattgc aagggcagtg gctggatgcc tccattcaac tcactggtgc
     1861 gttgggcggg ggttacaaac gctgatgata tgcgccttct atacttaaca tttattcagc
     1921 gttaagtgga gaactcgatg gcacaggtcg cgataattac cgcctccgat tcggggatcg
     1981 gcaaagagtg cgcgttatta ctggcgcagc aggggtttga tattggtatt acctggcact
     2041 cagatgaaga aggggcaaaa gatactgcgc gtgaggtggt tagccacggc gtacgtgcgg
     2101 agatcgtgca gctggatctc ggtaaactgc cagaaggagc actggcgctg gcgaaactca
     2161 ttcaacggct ggggcgtatt gatgtgctgg tgaataatgc gggcgcaatg accaaagcgc
     2221 cgtttcttga tatggctttc gatgagtggc gcaagatttt taccgttgat gtcgatggtg
     2281 catttttatg ctcgcaaatt gcggctcgtc agatggtgaa acaagggcag ggcggtcgca
     2341 tc
//
LOCUS       DAMZFT010000298         2337 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.300, whole genome shotgun sequence.
ACCESSION   DAMZFT010000298 DAMZFT010000000
VERSION     DAMZFT010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2337)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2337)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2337
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.300"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(139..552)
                     /locus_tag="PN977_005276"
     CDS             complement(139..552)
                     /locus_tag="PN977_005276"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6045169.1"
                     /translation="MGAKFLDQKTGVILEEPEFVKLYIRDLCRVKGLSATQYKIFTFM
                     LANMNTDNVVSFGPRTKEKFLSEHSIKPQTFNNNVSQLIESCLISRIARNEFLVNKKY
                     AVKVDWAKVQKIVWTTTYSNEGIDSTVTIAPKKTD"
     gene            1961..>2337
                     /locus_tag="PN977_005277"
     CDS             1961..>2337
                     /locus_tag="PN977_005277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001107325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein"
                     /protein_id="HDI6045170.1"
                     /translation="MTKNKRSGSEVRQRTKVITLRVNNRIANEIRRRAKSEGLTISEY
                     IRTTSLNNEIKQRVPSRYLDELIQLGRMQKKLFDKGKRPKDKEYLDVMHKIISLCAEM
                     KEVTQRISDIYKKMDLIKEEIKA"
BASE COUNT          697 a          487 c          487 g          666 t
ORIGIN      
        1 aaaaaaaata cataaaaata aatacccagg ttccaactta tttaattgag cagaacatag
       61 gtcttcataa aaatttaaag acctataata gaattagcga cattttgttt tatcgaaatc
      121 ttttacaatc ataagaaatt aatccgtttt tttcggggct atcgtcactg ttgaatcaat
      181 cccttcatta ctgtaagttg ttgtccatac tatcttttgt actttcgccc aatcaacttt
      241 aactgcgtat tttttattaa ccaaaaattc atttctggct attctagaaa ttaaacatga
      301 ctcaatcagt tgagacacat tattattaaa tgtttgtggt ttaattgaat gttctgataa
      361 aaacttttct ttggttcttg gtccaaatga aacaacatta tcagtattca tattagcaag
      421 catgaacgta aaaattttgt attgcgttgc tgatagccct ttaacacggc ataaatcacg
      481 gatatacagc ttcacgaact ccggctcttc caaaattacg ccagtttttt gatctaaaaa
      541 tttcgctccc attctcacct cgcaggtaat ttagttaccg ttttatcggt atcttatgta
      601 tgttccacaa ccgagtaaat actttttttc cggtatttga ttagcaacaa aaacagtatt
      661 ccggtatttg aaataccgct gagcggtatt tcaacctccg ctcagcggta aaaactttcc
      721 cgctgagcgg tatatctatt cccgctgagc ggtatttagt ttgcctctag gccttgtcac
      781 acctgatttt cctatctgtc tgtgaatcta tcttagatct cgtgcgtaag tgcactatgc
      841 caatactggt tcccttccgc ttcctcgccc actcgcgagc ctagcggctc ggtcgctggg
      901 gctgcggcga gcggtgccac ctctcgcata tcgttgacga tcccgatttg atctcaggcg
      961 atcgagtcgc tggccaccgg ttaaacgcgc tcaaagcctc ccagacgcac aaaacggggg
     1021 ttaaatgccc gtcagggcat ggaaggcgcg aaaaacgctt acagaggttt tcagaaggtt
     1081 aggcgcgccg atacccctaa ccaggggctt tcaggtcgct aggaaccgcg ccagccccct
     1141 gcaaggccca tttgcacgga acagacagcc actcggttgg taggtggtcg tagaccgtcg
     1201 gtgttacagc gatgggggtc tggggttctc cccagacggt ttccggttgc gctcaaggcg
     1261 ctgccggaac cgtggcggct tcgatggtga agggtgagcg cgtttccaag aggttttcca
     1321 ggcaagctat gactgaactg aacagattga acgaggagga aacgcgctca gacgctttgt
     1381 agcgcgcttt tctgtgtcag gcaagtttgt tgtgtctttt gcctttcagg ttgttagcag
     1441 ctggcggagc actgcacggg gattaatgct cccattgcta ggatgaaata aagattagga
     1501 gcgaagttaa gttatatttt taatatataa acgcgatatc aataactcac atagttatat
     1561 tttatgcata tgctaccatc ttgctctttt gcctcctgct ctctggacga atcccgcatt
     1621 accaacaggc aatggattat ataaccaaga agatctaatc tttattcctc taataatcca
     1681 caaacgacag acaacaaaga catcaattaa ttcggtgtta tttattaaat aacaagcaat
     1741 aatttttaaa atctgtgtaa ataatattga atgaagcatc atttttttgt atcagaaata
     1801 ccaggttacg acgcggtatt ttattttcgt cgaaaagttg cagcggaaaa tgggacagga
     1861 tgtgtattcg tcataccgcg aatacattcg cttgcatgag aatacctcct gtcagggcaa
     1921 gccccgacac cccaaacgtg caaaatggag aatcattgcg atgactaaaa ataagcgtag
     1981 cggttcagaa gtacggcaga ggactaaggt tatcaccctt agagtaaata acagaatcgc
     2041 aaacgaaatc agacggcgag cgaagagcga agggctgact atttcagaat atataaggac
     2101 aacaagctta aataatgaaa taaaacagcg cgttccttcc agatatttag atgaattaat
     2161 ccaacttggt cggatgcaga agaaattatt tgataaaggg aagagaccga aggacaaaga
     2221 gtatctggat gtgatgcata aaataatttc actttgtgct gaaatgaaag aagtaacaca
     2281 gcgaatttct gatatatata aaaaaatgga tttaataaaa gaagaaataa aagcaat
//
LOCUS       DAMZFT010000299         2331 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.301, whole genome shotgun sequence.
ACCESSION   DAMZFT010000299 DAMZFT010000000
VERSION     DAMZFT010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2331)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2331)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2331
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.301"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            192..821
                     /gene="sxy"
                     /locus_tag="PN977_005278"
     CDS             192..821
                     /gene="sxy"
                     /locus_tag="PN977_005278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRP-S regulon transcriptional coactivator Sxy"
                     /protein_id="HDI6045171.1"
                     /translation="MKSLSYKRIYKSQEYLATLGTIEYRSLFGSYSLTVDDTVFAMVS
                     DGELYLRACEQSAQYCVKHPPVWLTYKKCGRSVTLNYYRVDESLWRNQLKLVRLSKYS
                     LDAALKEKSTRNTRERLKDLPNMSFHLEAILGEVGIKDVRALRILGAKMCWLRLRQQN
                     SLVTEKILFMLEGAIIGIHEAALPVARRQELAEWADSLTPKQEFPAELE"
     gene            complement(784..>2331)
                     /gene="yccS"
                     /locus_tag="PN977_005279"
     CDS             complement(784..>2331)
                     /gene="yccS"
                     /locus_tag="PN977_005279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415480.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01666 family membrane protein"
                     /protein_id="HDI6045172.1"
                     /translation="LALANGQLMATLNQTKLSLLTRLRGDRGQRGTRRTLHYYFVAQD
                     IHERASSSHIQYQTLREHFRHSDVLFRFQRLMSMQGQACQQLSRCILLRQPYQHDPHF
                     ERAFTHIDAALERMRDNGAPADLLKTLGFLLNNLRAIDAQLATIESEQAQALPHNDDE
                     NELADDSPHGLSDIWLRLSRHFTPESALFRHAVRMSLVLCFGYAIIQITGLHHGYWIL
                     LTSLFVCQPNYNATRHRLKLRIIGTLVGIAIGIPVLWFVPSLEGQLVLLVITGVLFFA
                     FRNVQYAHATMFITLLVLLCFNLLGEGFEVALPRVIDTLIGCAIAWAAVSYIWPDWKF
                     RNLPRMLERATEANCRYLDAILEQYHQGRDNRLAYRIARRDAHNRDAELASVVSNMSS
                     EPNVTPQIREAAFRLLCLNHTFTSYISALGAHREQLTNPEILAFLDDAVCYVDDALHH
                     QPADEERVNQALAGLKQRMQQLEPRADSKEPLVVQQVGLLIALLPEIGRLQRQITQVP
                     QETPVSA"
BASE COUNT          623 a          555 c          600 g          553 t
ORIGIN      
        1 tatgaatgta cagtacatcc agtgacaaca aagatcaacc ctattttcgg aaagagcctc
       61 gcaaattttg tcgttggtga cgggaaaaca taaattaatc ttgcccctta agaataagtt
      121 gcctattttc gtagttaacg gatccgttaa tgtgaatcat tcttttatgt tatgatttta
      181 aaaggaattt tatgaaaagc ctctcctata agcggatcta taaatcacaa gaatacctgg
      241 caacgttggg cacaattgaa taccgatcat tgtttggtag ttacagcctg actgttgacg
      301 acacggtgtt tgcgatggtt tctgatggtg agttgtatct tcgggcttgt gagcaaagtg
      361 cacagtactg tgtaaaacat ccgcctgtct ggctgacata taaaaagtgt ggccgatccg
      421 ttaccctcaa ttactatcga gttgatgaaa gtctatggcg aaatcaactg aagctggtgc
      481 gtctgtcgaa atattctctc gatgcagcgc tgaaagagaa aagcacgcgc aatacccggg
      541 aaagactgaa agatttgccc aatatgtctt ttcatctgga agcgattctc ggggaggtgg
      601 ggattaagga tgtacgggcg ttacgtatac ttggggcaaa aatgtgttgg ttgcgactgc
      661 ggcagcaaaa cagtctggtg acagaaaaga ttctgtttat gcttgaaggg gccattatcg
      721 gcattcatga agctgcgctc ccggtggctc gccgccagga gcttgcagaa tgggctgact
      781 ctcttacgcc gaaacaggag tttcctgcgg aacttgagta atctggcgtt gcagacgacc
      841 aatctctggc agtaatgcaa tcaataaccc aacttgttgt acgaccagag gttctttgct
      901 gtctgcccgt ggttcaagtt gctgcatccg ctgtttcagg ccagctaatg cctgattgac
      961 gcgttcttca tcagcaggtt gatgatgtaa cgcgtcatca acatagcaaa ctgcgtcatc
     1021 aagaaacgcc agaatttcag gattagttaa ctgctcccgg tgagcaccga gggctgagat
     1081 atagctggta aacgtatggt taaggcacag caaccgaaac gcggcctcgc gaatttgcgg
     1141 ggtaacgttc ggctcgctgg acatatttga taccaccgac gccagttcag catcacggtt
     1201 gtgtgcatcg cggcgggcaa tacgatacgc cagacggtta tcacgcccct gatggtattg
     1261 ctccagtatg gcatcgagat agcgacagtt ggcctcggtg gcacgttcga gcatgcgcgg
     1321 cagattgcga aacttccagt caggccagat gtagctcacc gccgcccacg caatggcaca
     1381 accaatcagc gtatcgatta cgcgaggtaa cgccacttca aaaccttcac ccagcaagtt
     1441 aaaacaaagt agcaccaaaa gtgtgatgaa catcgttgca tgggcatatt gcacgttacg
     1501 gaaggcaaaa aagagcacgc cggtaataac cagcagcacc agctgccctt ccagtgacgg
     1561 cacaaaccac agcacaggaa tgccaatcgc gatacctacc agcgtaccaa taatccttaa
     1621 tttcaggcgg tggcgcgtgg cgttatagtt tggctggcag acaaacaaac ttgtcagcaa
     1681 gatccaatac ccgtgatgca gtccggttat ctgaatgatg gcgtagccga agcacaacac
     1741 cagcgacatt cttaccgcat gacggaagag ggcggattcc ggcgtgaagt gacggctaag
     1801 acgcagccag atatcactca atccgtgcgg gctgtcatca gcgagctcat tttcgtcatc
     1861 attatggggc agtgcctggg cctgttctga ttcaattgtt gccagttggg catcaatggc
     1921 acgtaaattg ttcagtaaaa atcccagtgt tttgagtaaa tcagcaggcg cgccgttatc
     1981 gcgcatccgc tccagcgcag catcaatatg cgtaaaagcg cgctcaaaat gcggatcatg
     2041 ttgataaggc tgacgcagca aaatacagcg tgacagttgc tggcacgcct ggccctgcat
     2101 cgacatcagc cgctgaaaac ggaacagcac gtcgctgtgg cgaaaatgtt cacgcaatgt
     2161 ttgatactga atatgagaag agctggcacg ctcgtgaata tcctgcgcga caaagtaata
     2221 atgcagtgtg cgacgcgttc cccgttgacc acgatcgcca cgtaagcggg tcagcagcga
     2281 gagtttcgtc tgattcaatg tcgccatcag ctgaccgttg gcgagagcca a
//
LOCUS       DAMZFT010000300         2328 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.302, whole genome shotgun sequence.
ACCESSION   DAMZFT010000300 DAMZFT010000000
VERSION     DAMZFT010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2328)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2328)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2328
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.302"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            1162..1689
                     /locus_tag="PN977_005280"
     CDS             1162..1689
                     /locus_tag="PN977_005280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001279566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication/maintenance protein RepL"
                     /protein_id="HDI6045173.1"
                     /translation="MSVERYKSNPFLENMIVPVKGRQVKLSRLGKDNNILMNQSTGEF
                     LGTHVTTYKQVDADQFIKIFTDNIAMTFDFTSAGIKAFGVLLWAVQHHALSKDEVDLD
                     SFILDAFISENNKENKKIRLSMATFKRGINELEKASIIAKTLRKGRYYINPNFVFNGD
                     RIAFTNLIERKAKDK"
     gene            complement(1731..2156)
                     /locus_tag="PN977_005281"
     CDS             complement(1731..2156)
                     /locus_tag="PN977_005281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001107325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein"
                     /protein_id="HDI6045174.1"
                     /translation="MVKNKRNGSETRQRSKVITLRINNKIDREIRRLAKAEGMTISEY
                     IRTSCLNKEIIQRIPSRYLDELLELGRMQKNLFDKGRTPKDKDYLDVMHKIITLCAEA
                     KEVILRNSDIYKEMDLLKQEIKIIKQYYKNKYPGSDLFN"
BASE COUNT          676 a          494 c          479 g          679 t
ORIGIN      
        1 aaaaaatgat gcttcattca atattattta cacagatttt aaaaattatt gcttgttatt
       61 taataaataa caccaaatta atttatatct ttgttgtctg tcgtttgtgg attattggag
      121 gaataaagat tagatcttcc ttgttatata atccattgcc tgttagtgat acggagtgtg
      181 tccaaggaac aggaagcgga agagcaagat agtagcatgt gcataaaata taaatccgtg
      241 agttattgat atcgcgttta tatattaaaa atataactta acattgctct taatatttat
      301 tttgtcctgg caatgggagc attaatcctc gtgcagtgct ccgccagctg ctgacaacct
      361 cgacatataa ccatcgatac gatagaacat cagggcgcgt ttccggcaag aactaaccat
      421 ggggatggtt tgcgtgccaa cgaacgccgg aaatgcgcct caaacctacc gagtcgccac
      481 ggttccggca gcgccttgag cgcaaccgga aaccgactgg ggagaacccc agacccccac
      541 cgctgcaaca tcatcggtct acgaccacct accaaccgag tggctacctg tgccgtgcaa
      601 acaggctttg cagggggctg gcgcggttcc tagcgacctg aaagcccctg gttaggggta
      661 tcggcgcgtc tggcctcctg aaaccctctg taagcgtttt tcgcgccttc catgccctca
      721 cgggcattta aaccccgttt cgtgcgtttg agaggctctg agcgcgtttt agggccatgc
      781 tgacgactca tcttcgaaag aacaacacca aaagccagcc actacgagag aggaggatcc
      841 gctcgccgca gccccagcga ccgaacgtta gtgagcgagt gggcgaggaa gcggaagtga
      901 accagatgtc agcctagggc acttacgcac caccgtataa gaactaagaa gggaatcctc
      961 tgcaacccgc atgaaatggg gatcgtgatc ttcggatctg taacttgtta gctcaaccct
     1021 gttaccaaca cggctcaaaa taggtgttgt ttttgagccg tgttagctat agtgtgagct
     1081 ttcaaagctc aaatagttcg ttcattatga gctttgaaag ggcgcttttt tagctactaa
     1141 ggttcaatat aatggtgaat tatgagcgtt gaaaggtaca aatcgaaccc atttttggaa
     1201 aatatgatcg tgcctgtcaa aggtaggcag gttaaattat ctcgtttagg caaagataat
     1261 aatatattaa tgaatcaatc gacgggagag ttccttggta ctcacgtgac aacatataaa
     1321 caagtcgacg ccgatcaatt tatcaaaata tttacagata atatagcgat gacatttgat
     1381 tttacttccg ccggaattaa agcttttggt gtccttcttt gggcggttca gcatcatgca
     1441 ttgtctaaag atgaagttga tttagatagt tttatattgg atgccttcat ctctgaaaat
     1501 aataaagaaa acaagaagat aaggttgagt atggccacat ttaaaagggg cattaatgaa
     1561 cttgagaaag caagtataat agcaaaaacc ctaagaaaag gacgttatta tataaaccca
     1621 aactttgttt ttaatggtga tagaatagcc ttcacaaatc tgatagaaag aaaagcaaaa
     1681 gacaaataac aacatataaa ccccgctcta aaaattgagc agggtttatt ttaattaaat
     1741 aaatcagagc ctgggtattt atttttgtag tactgtttaa ttatttttat ttcctgtttt
     1801 aataaatcca tctctttata tatatcagaa ttacgtaaaa taacctcctt cgcttctgca
     1861 cacagcgtga ttattttgtg catcacatca aggtagtcct tatccttcgg tgttcttcct
     1921 ttatcaaata aattcttctg catccggcca agctctaata attcatccaa atatctggaa
     1981 gggatacgct gtattatttc tttatttaaa catgacgttc ttatatattc tgaaatcgtc
     2041 atcccttccg cctttgccag gcgtcggatc tccctgtcaa tcttgttatt tatccgcaag
     2101 gttataacct tgcttctctg ccgtgtttct gaaccgttgc gcttattttt aaccatcaca
     2161 atgaacctct ggtttccacg ttcggggtgt cggggcctgc cctgacagga ggtattttca
     2221 tacaagcgaa tgtattcgcg gtatgacaaa tacacatcct gtcccatttt ccgctacaac
     2281 ttttcgacga aaataaaata ccgcgtcgta acctggtatt tctgatac
//
LOCUS       DAMZFT010000301         2296 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.304, whole genome shotgun sequence.
ACCESSION   DAMZFT010000301 DAMZFT010000000
VERSION     DAMZFT010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2296)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2296)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2296
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.304"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            1141..1962
                     /locus_tag="PN977_005282"
     CDS             1141..1962
                     /locus_tag="PN977_005282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001696722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraE"
                     /protein_id="HDI6045175.1"
                     /translation="MKTDSTALAARMILSLDRERICECLLSNRQLQSTPLQVRYPQGV
                     RDALGIMSEQLSLSVSDLTRILVEDALSEMFLPADNIVRRLLSRMEHIMQAYDISATT
                     MAALLAPWNIRPAVFREPDRLIDYLTGDILVALADWFYLSPEWLNGRVHYPLYRPGDW
                     PETEDAFCRLISDSDNIDIILWHGFPFSGEHAQEYCGVLLRQKKEINNTIIYPVLSLY
                     PVRMDIEKEGWFQMARKISPDIPVRAVMLTPAQAEYLITGKILPTALFRVPLSPW"
     gene            2046..>2296
                     /locus_tag="PN977_005283"
     CDS             2046..>2296
                     /locus_tag="PN977_005283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003557916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraF"
                     /protein_id="HDI6045176.1"
                     /translation="MMMKKHITRTLIASAVLFSFNSTAATSYFEARNDAMGGTGVASS
                     HYGVAPLANPALLTKSGAKDDFSLLLPSVGAQLSDPGNIA"
BASE COUNT          665 a          537 c          467 g          627 t
ORIGIN      
        1 ctgctgatgg gcgagatgat ggatgcaaaa taaacattca ttgccccggg acataaaaat
       61 agagccggaa acgccgggca cttgcgtgct gctcagagga gagttgccca caaacattga
      121 tgaacggggc atgatttatc cggtaacaaa ctgaagacat atcagcatga atataaacta
      181 ttgcttccgg gttaaattaa tcacggtcat cctgtctcat ttcctgatac agatatatgt
      241 gtggacagat tttctgacaa cgaaaaatat tccccgtctc attgccgcta tggtagccct
      301 gtacctgtct gcagtaactg tcattccggc atttttactg tcatatgaca gtgagagatt
      361 taaaatactc tcacctgtaa agcaaatcat ttattagtat tatatactgg catcgtcatt
      421 ttttattatc accggaattc aacgaataaa tataacacca ctgcaaaata tggagtaaaa
      481 atgatactca tctcatcagt aaatacgggg gaaattacag aaaaaacgaa caaagttctt
      541 gatatgctgt atgaatacaa cataccgata ctttatggct tctcagaaga agtacgggtt
      601 ttgagaaact gcgtcaactg taatatcaat gatgactacg atgagagtga ctataatttt
      661 atggtatatg gtgacgcagt attaaacatg gtaaaaaatt atttgctatt agatatagca
      721 tcataaaagc cgatcctgac aaggtattaa tcaacgttat ctccgtatct attgttgaac
      781 tgactgaatt atttaatcaa cataatcaat atatcttatt caaaccagaa acaggcaaaa
      841 aatcacaaac aaaaaactat attattctct ttttaattac tgctgttact ctgtgtattt
      901 ttcttcagtt catgagcaaa ctcaccagtt aaaaacgccc gacgtacttt atgccatcct
      961 cccggacagc ttcacttgtt ttaccactcc tcaggtgaca gggcatgacc tccacacaga
     1021 gtgcccgaca ctcctgaaac acactcgatc ataacgatcg aaataactcg cttccgttta
     1081 aaaaacactc tatttagact caatttatga acaaaacacg ctgacccgca tggaggtgaa
     1141 atgaaaactg acagcacagc tcttgccgcc cgtatgatac tctccctgga cagggagcga
     1201 atttgcgaat gcctgctcag caatcgccag ctgcagtcca cacccctgca ggttcgttat
     1261 ccgcagggag tcagggatgc ccttggtatc atgagcgaac agctttctct ttccgtctct
     1321 gacctgacac gtatcctggt cgaagacgcg ttaagtgaaa tgtttcttcc tgcagataac
     1381 atcgtccgcc gtcttctcag caggatggag cacatcatgc aggcatatga tatcagcgca
     1441 acaacgatgg ccgcattact ggccccctgg aatatccgcc ctgccgtctt ccgggagccg
     1501 gacagactca ttgactatct gaccggggat attcttgtag cactggctga ctggttttat
     1561 ctcagtccgg aatggctgaa cggcagggtg cattatccgc tgtatcgtcc aggcgactgg
     1621 cctgaaacag aggacgcgtt ctgtcgtctt atatcagaca gcgataatat cgacattatt
     1681 ctctggcatg gttttccttt ttccggtgaa cacgctcagg aatattgcgg cgttcttctc
     1741 cgccagaaaa aagaaattaa taataccatt atttatccgg tactttcgtt atatccggtc
     1801 agaatggata tagaaaaaga aggctggttt cagatggccc gaaaaatatc tccggatatc
     1861 cctgttcgcg ccgtaatgct cactccggca caggcagaat atctgataac agggaaaata
     1921 ttacccactg cgttattcag ggttccgtta tcaccctggt aataaaataa tgcaccaact
     1981 gtttttaatg gcacagccgt gccctctgtt gttataccca aaatacctca atacaggaaa
     2041 ctgaaatgat gatgaaaaag catattaccc gtactctcat cgcgtctgca gttttatttt
     2101 cctttaattc gactgcagca acgtcatatt ttgaagcccg caatgatgcg atgggaggaa
     2161 cgggggttgc ctcctctcac tatggtgtgg ccccactggc aaacccggca ctgctgacaa
     2221 aatccggtgc aaaagatgac ttcagtcttc tgcttccgtc cgtgggtgcg cagctgtccg
     2281 atccgggtaa tatcgc
//
LOCUS       DAMZFT010000302         2267 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.305, whole genome shotgun sequence.
ACCESSION   DAMZFT010000302 DAMZFT010000000
VERSION     DAMZFT010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2267)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2267)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2267
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.305"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            190..441
                     /locus_tag="PN977_005284"
     CDS             190..441
                     /locus_tag="PN977_005284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001595120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HDI6045177.1"
                     /translation="MPSIILSDTSASVSELKKNPMATVSAGEGYPVAILNRNQPAFYC
                     VPAELYERMLDALDDQELVKLVTERSNQPLHDVDLDSYL"
     gene            438..725
                     /locus_tag="PN977_005285"
     CDS             438..725
                     /locus_tag="PN977_005285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020317119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HDI6045178.1"
                     /translation="MTYTVKFRDDALKEWLKLDKSIQQQFAKKLKKCSENPHIPSAKL
                     RGLKDCYKIKLRASGFRLVYQVIDDMLIIAVVAVGKRERSNVYNLASERMR"
     gene            829..1419
                     /locus_tag="PN977_005286"
     CDS             829..1419
                     /locus_tag="PN977_005286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001532129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine acetyltransferase"
                     /protein_id="HDI6045179.1"
                     /translation="MMKNPLAYFHIATFLLSGKSRNLSEYWKAEVIMRDKFVLHRLIR
                     NGMQRQRGFLMWWRLANEMFISGNKKQRKCAIKIKNALMERYGCDIGLGARIGKGLVL
                     PHHAGIVIHGNVDIGENVIIRQNTTIGEKVSDSRENYIVIGDNVDIGAHTCIIGLNVK
                     IGSNVKIGAMSFIMEDVPDNCTYVTRKESRVIIREQ"
     gene            1522..2127
                     /locus_tag="PN977_005287"
     CDS             1522..2127
                     /locus_tag="PN977_005287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001532130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6045180.1"
                     /translation="MGSVTKKPSPVVIIKKKRRILSHQDDAVQAVPDDIKPAPVMEFK
                     TDSLPPEIPATVAGSPEQQKKTGRKKKKKKNNRWNHEFIRHSLDCVKKTFPQLRAEEG
                     GYHAFKIGISKDVMAFISASPECGLTVKEWNCAVRMITGGYKYLQRISVAGTPRYGLD
                     GKVHGTVTEEEALYAQAKLEKHTQRYNARMKETEDARCNQS"
BASE COUNT          696 a          424 c          527 g          620 t
ORIGIN      
        1 ccggcttacg ccgggcttcg gcggttttac tcctgtgcca tatgctacaa cggagtgccg
       61 ccttccatgc cgctggcgcg gcatcagaac aactgaagag tgacggcgca cattagcatc
      121 aaacagactt gttaagcagt ctttcagggt gtaatctgaa gacctgttaa acagtctcag
      181 aggtgtgtta tgcccagtat aattttgtct gacaccagtg caagcgtcag tgaactgaaa
      241 aaaaatccaa tggcgacagt cagtgccgga gagggttatc ctgttgccat tcttaaccgg
      301 aatcagcctg cattctactg tgttccagct gagctctatg aaaggatgct tgatgccctg
      361 gatgaccagg aactggtgaa actggttact gaacgcagca atcagccact gcatgatgtg
      421 gacctggaca gttatttatg acttatacag taaaattcag ggatgatgcg ctcaaagaat
      481 ggctgaaact ggataaatcc attcaacagc agtttgcgaa aaaactaaag aaatgcagcg
      541 aaaatcctca tattccttcc gcaaaactgc gtggattaaa agactgctac aaaatcaaac
      601 ttcgcgcatc ggggtttcgt ctggtctatc aggttattga tgacatgcta attattgcgg
      661 ttgtcgcggt aggtaaacgt gaacgtagta acgtttataa tctcgccagt gagagaatga
      721 gatagttatc tttaaggctg actgatatta cttcaaaagt atttctgata gttatgttat
      781 tttattccac aatgcagaat atattcttta ttctttaagg tcgtttttat gatgaaaaat
      841 ccattagctt attttcatat tgccaccttt ttattgtcag gtaaaagtcg taatctgtca
      901 gaatactgga aagccgaagt catcatgcgg gataagtttg tcttacaccg cctgataaga
      961 aacgggatgc agagacaacg aggctttctg atgtggtggc gtttggccaa tgagatgttt
     1021 atctctggaa acaaaaaaca aaggaaatgt gcgataaaga taaaaaatgc acttatggaa
     1081 cggtatgggt gtgatatcgg cctgggggcg cggattggta aaggtctcgt gttgcctcat
     1141 catgcaggta tcgtcattca tggtaatgtt gatatcggtg agaatgtcat tatacggcag
     1201 aatacaacca ttggtgaaaa ggtttctgac agcagggaaa actatattgt tattggcgat
     1261 aatgtcgata ttggtgccca tacatgtatt attgggttaa atgtgaaaat cggaagtaat
     1321 gttaaaatcg gggcaatgtc ttttattatg gaagatgtac ctgataactg tacttatgtc
     1381 accaggaagg aatccagagt gataattcgc gaacagtaat gaaatacatg ttatcattct
     1441 tctgttggtg actttgccga ctcagatata tactatctgt atgtcgtgca ttatatatta
     1501 acgatacgca gaggtgtgta tatgggcagt gttacaaaga aaccgtcacc tgttgttatt
     1561 attaagaaaa aaagacgaat tctttctcat caggatgatg ccgtacaggc agttcctgat
     1621 gatataaaac cagcgccagt aatggaattt aaaaccgact cattacctcc tgaaatacca
     1681 gccacagtgg ccggttcccc ggaacaacag aaaaaaacag gacgtaagaa gaaaaaaaag
     1741 aagaacaata gatggaatca tgagtttatc cgacacagtc tggactgtgt taaaaaaaca
     1801 ttccctcagt tacgggctga agagggggga tatcatgcat ttaaaatagg catctcaaag
     1861 gatgtcatgg cttttatttc ggccagtcct gagtgtggac taactgtaaa agaatggaat
     1921 tgtgctgtaa ggatgattac tggcggttac aaatatctgc agcgtatatc agtcgcaggt
     1981 actccgcgtt atggtctgga tggcaaagtt catggaactg taacggagga agaagcgctg
     2041 tatgcgcagg caaaacttga aaaacacacg cagcgttata acgccaggat gaaagagaca
     2101 gaagatgctc gttgcaacca atcataaacg tacacgtcag cacacgacaa acatgcttca
     2161 aaaatcgact aattcgctgt gtcggaaatt tcggcggttc cggcgtagaa ccccctatta
     2221 atatttgttt taggttctat tcaacatagg tcatcctaaa acacatt
//
LOCUS       DAMZFT010000303         2097 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.306, whole genome shotgun sequence.
ACCESSION   DAMZFT010000303 DAMZFT010000000
VERSION     DAMZFT010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2097)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2097)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2097
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.306"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(<1..182)
                     /locus_tag="PN977_005288"
     CDS             complement(<1..182)
                     /locus_tag="PN977_005288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tunicamycin resistance protein"
                     /protein_id="HDI6045181.1"
                     /translation="MIIWINGPFGAGKTTLAKRLRDRRSKSLIFDPEEIGFVVKETVP
                     MPASGDYQDLPLWRGLT"
     gene            complement(195..1055)
                     /gene="aac(3)-IId"
                     /locus_tag="PN977_005289"
     CDS             complement(195..1055)
                     /gene="aac(3)-IId"
                     /locus_tag="PN977_005289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000557454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(3)-IId"
                     /protein_id="HDI6045182.1"
                     /translation="MHTRKAITEALQKLGVQTGDLLMVHASLKAIGPVEGGAETVVAA
                     LRSAVGPTGTVMGYASWDRSPYEETLNGARLDDEARRTWLPFDPATAGTYRGFGLLNQ
                     FLVQAPGARRSAHPDASMVAVGPLAETLTEPHELGHALGEGSPVERFVRLGGKALLLG
                     APLNSVTALHYAEAVADIPNKRWVTYEMPMLGRDGEVAWKTASDYDSNGILDCFAIEG
                     KPDAVETIANAYVKLGRHREGVVGFAQCYLFDAQDIVTFGVTYLEKHFGTTPIVPPHE
                     AVERSCEPSG"
     gene            1198..>2097
                     /locus_tag="PN977_005290"
     CDS             1198..>2097
                     /locus_tag="PN977_005290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001339197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS4-like element ISVsa5 family transposase"
                     /protein_id="HDI6045183.1"
                     /translation="MCELDILHDSLYQFCPELHLKRLNSLTLACHALLDCKTLTLTEL
                     GRNLPTKARTKHNIKRIDRLLGNRHLHKERLAVYRWHASFICSGNTMPIVLVDWSDIR
                     EQKRLMVLRASVALHGRSVTLYEKAFPLSEQCSKKAHDQFLADLASILPSNTTPLIVS
                     DAGFKVPWYKSVEKLGWYWLSRVRGKVQYADLGAENWKPISNLHDMSSSHSKTLGYKR
                     LTKSNPISCQILLYKSRSKGRKNQRSTRTHCHHPSPKIYSASAKEPWVLATNLPVEIR
                     TPKQLVNIYSKRMQIEETFRDLKS"
BASE COUNT          541 a          581 c          492 g          483 t
ORIGIN      
        1 gtaagtcccc tccacaaggg gagatcctga tagtctccgc tcgctggcat ggggaccgtt
       61 tctttcacca cgaacccgat ttcctcgggg tcaaagatca gcgatttgga acgccgatcg
      121 cgcagccgct tagcgagcgt cgtctttccg gcgccgaaag gtccgttgat ccagattatc
      181 attgtcgacg gcctctaacc tgaaggctcg caagagcgct cgacggcctc gtgcggaggc
      241 acgatcggag tggttccgaa atgcttctca agataggtga cgccgaacgt cacgatgtcc
      301 tgcgcgtcga acaggtagca ctgagcaaag cccacgacac cttctcgatg gcgaccgagc
      361 ttcacgtaag catttgctat agtttcaacc gcatccggct ttccttcgat agcaaagcaa
      421 tcgagaatgc cgtttgaatc gtaatccgat gccgttttcc aggcgacttc accgtctctt
      481 ccaagcatcg gcatctcata cgtcacccac cgtttgttgg ggatatcggc aaccgcctcg
      541 gcgtagtgca atgcggtaac ggagtttagc ggcgcaccca acagcagggc cttcccgcca
      601 aggcgaacga accgctcgac gggcgatcct tcccccaagg cgtgaccgag ttcgtgaggc
      661 tccgtcagcg tttcagccag cggaccaacc gcgaccatcg atgcatcggg gtgcgcgctg
      721 cgccgcgcgc cgggggcttg aaccagaaat tgattcagca ggccgaaccc acggtaagtc
      781 ccggctgttg cgggatcgaa cggcagccag gtacggcggg cttcgtcatc cagccgagcg
      841 ccattcagag tctcctcgta gggtgatcgg tcccacgacg cgtatcccat cacagtgcca
      901 gtcggcccaa ccgcggagcg taacgcggca acgaccgtct ccgctcctcc ttcgaccgga
      961 ccaatcgctt taagtgaggc atgcaccatc aagaggtcac cggtttggac tccgagtttt
     1021 tgaagcgcct ccgttattgc cttccgcgta tgcatcgcga tatctcctct aaactgcaaa
     1081 acactatacg ctgatgaatc ccctaatgat tttggtaaaa atcattaagt taaggtggat
     1141 acacatcttg tcatatgatc aaatggtttc gcgaaaaatc aataatcaga caacaagatg
     1201 tgcgaactcg atattttaca cgactctctt taccaattct gccccgaatt acacttaaaa
     1261 cgactcaaca gcttaacgtt ggcttgccac gcattacttg actgtaaaac tctcactctt
     1321 accgaacttg gccgtaacct gccaaccaaa gcgagaacaa aacataacat caaacgaatc
     1381 gaccgattgt taggtaatcg tcacctccac aaagagcgac tcgctgtata ccgttggcat
     1441 gctagcttta tctgttcggg caatacgatg cccattgtac ttgttgactg gtctgatatc
     1501 cgtgagcaaa aacggcttat ggtattgcga gcttcagtcg cactacacgg tcgttctgtt
     1561 actctttatg agaaagcgtt cccgctttca gagcaatgtt caaagaaagc tcatgaccaa
     1621 tttctagccg accttgcgag cattctaccg agtaacacca caccgctcat tgtcagtgat
     1681 gctggcttta aagtgccatg gtataaatcc gttgagaagc tgggttggta ctggttaagt
     1741 cgagtaagag gaaaagtaca atatgcagac ctaggagcgg aaaactggaa acctatcagc
     1801 aacttacatg atatgtcatc tagtcactca aagactttag gctataagag gctgactaaa
     1861 agcaatccaa tctcatgcca aattctattg tataaatctc gctctaaagg ccgaaaaaat
     1921 cagcgctcga cacggactca ttgtcaccac ccgtcaccta aaatctactc agcgtcggca
     1981 aaggagccat gggttctagc aactaactta cctgttgaaa ttcgaacacc caaacaactt
     2041 gttaatatct attcgaagcg aatgcagatt gaagaaacct tccgagactt gaaaagt
//
LOCUS       DAMZFT010000304         2091 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.307, whole genome shotgun sequence.
ACCESSION   DAMZFT010000304 DAMZFT010000000
VERSION     DAMZFT010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2091)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2091)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2091
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.307"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            37..369
                     /locus_tag="PN977_005291"
     CDS             37..369
                     /locus_tag="PN977_005291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nikA protein"
                     /protein_id="HDI6045184.1"
                     /translation="MSEKKTRSGSEKRQKNVLIAVRFSPEEAEIVKEKAEKNGLTVST
                     LIRKTVLGKQINARIDEDFLKELMRLGRLQKHLFVEGKRTGDKEYAEVLVAITELANT
                     LRRDLMGR"
     gene            381..>2091
                     /locus_tag="PN977_005292"
     CDS             381..>2091
                     /locus_tag="PN977_005292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001387486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HDI6045185.1"
                     /translation="MNAIIPKKRRDGKSSFEDLIAYTSVRDDVPEDELRPDTEMKAEI
                     PHRNRFRRLVDYATRLRNEKFVSLIDVMKDGSQWVNFYGVTCFHNCLSLESAAEEMQK
                     AADLAHFSKDDTDPVFHYILSWPAHESPRSEQLFDCVRHTLKSLELSKHQYVAAVHTD
                     TDNLHVHVAVNRVHPETGYINCLPWSQEKLSRACRELELKHGFAPDNGCFVHAPGNRI
                     VRKTALVRERRNAWRRGKKQTFREYIAQMSIAGLREEPAQDWLSLHKRLASDGLYITM
                     QEGELVVKDGWDRAREGVALSSFGPSWTAEKLGRKLGEYQPVPTDIFSQVGTPGRYDP
                     EAINVDIRPEKVAETESLKQYACRHFAERLPEMARNGELKSCLAVHRTLAEAGLWMGI
                     QHGHLVLHDGFDKQQTPVRADSVWPLMTLDYMQDLDGGWHSVPKDIFTQVIPGERFRG
                     HSIGTQAVSDYEWYRMRMGTGPQGAIKRELFSDKESLWGYALIQCRSQIEEMIAGGDF
                     SWQACHEMFARKGLMLQKQHHGLVIVDAFNHELTPVKASSIHPDLTLSRAEPQAGPFE
                     IAAA"
BASE COUNT          559 a          443 c          598 g          491 t
ORIGIN      
        1 agggcttagc cccgacaccc cggtaaggag cagaccatga gtgagaagaa aacccgaagt
       61 ggcagtgaga aacggcaaaa gaatgtcctc attgctgttc gtttttcacc agaagaggct
      121 gagattgtta aagaaaaggc agaaaaaaac ggactgacag tttcaactct gataagaaaa
      181 actgtgctgg gaaaacagat aaatgcccgc attgatgaag attttttgaa agagctgatg
      241 cgtcttggtc gcctgcagaa gcatctgttt gtggaaggaa aacgcacggg agataaagag
      301 tatgcagagg ttctcgttgc tattacagag cttgctaata ctctgcgaag agatttaatg
      361 ggacgctgag ataatcgctg atgaatgcca tcattccgaa aaaaagacgc gacggaaagt
      421 cttcttttga agacctgatt gcgtacacct cagtgcggga tgatgtgccg gaagatgaac
      481 tcaggccaga cacggaaatg aaagctgaga ttccgcaccg gaaccgcttc agacgtcttg
      541 ttgattatgc aacacgtctg cgtaatgaaa aatttgtttc cctgattgat gtgatgaaag
      601 acggcagtca gtgggtgaat ttctatggtg tgacctgctt tcataactgt ttgtctcttg
      661 aatctgcggc tgaagaaatg cagaaagcag cagaccttgc tcatttttcc aaagatgata
      721 cagatcctgt ttttcactat attctctcct ggcctgcgca cgaaagtccg cgttctgagc
      781 agctttttga ctgtgtgcgt cacaccctga aatcccttga gctgtcaaaa catcagtatg
      841 tggcagccgt tcatacagac acggataacc tgcatgtaca tgttgcagtg aatcgtgtcc
      901 accctgaaac cggctatatc aattgccttc cgtggagcca ggaaaaactc agtcgtgcat
      961 gccgtgaact tgaacttaag cacggattcg cgccggataa tggctgcttt gtgcatgcac
     1021 ccggcaaccg tattgtgcgc aaaacagcgc tggtacgtga gcgccgtaat gcctggcgac
     1081 gcgggaaaaa gcagacgttc agggaatata ttgctcagat gtcgattgcc gggctgcgtg
     1141 aagagcccgc acaggactgg ttatctttgc ataaacggct ggccagtgat ggtctgtata
     1201 tcacgatgca ggaaggggaa ctggtggtga aggatggctg ggacagagcg cgggaaggtg
     1261 ttgcactcag ttcgttcgga ccgtcatgga cagctgaaaa actggggcgg aaactggggg
     1321 aatatcagcc tgtaccgaca gatattttca gtcaggtggg gacacccggt cgttatgatc
     1381 cggaagccat aaacgtggat atccggccgg aaaaagtggc agaaacggag agtctgaaac
     1441 agtacgcctg tcgtcacttt gctgaacgct taccggaaat ggcgcgaaat ggtgaactga
     1501 aaagttgcct ggctgtacac cgtactctgg cagaggcggg attatggatg gggatccagc
     1561 acgggcatct ggtactgcat gatggatttg ataaacaaca gacaccagta cgtgcagaca
     1621 gtgtgtggcc gttaatgacg ctggattata tgcaggatct ggatggtgga tggcactctg
     1681 taccgaagga tatttttact caggttatac ccggagaacg attccgggga cacagtattg
     1741 gcactcaggc tgtcagcgat tacgaatggt atcgcatgag gatgggcact ggtccacagg
     1801 gtgccataaa gcgtgaactg ttttccgata aggaaagtct gtggggatac gctcttattc
     1861 agtgcaggag ccagattgaa gagatgatag ctggcgggga tttcagctgg caggcatgtc
     1921 atgagatgtt tgcgcgtaaa gggcttatgc tgcagaaaca gcatcatggt ctcgtgattg
     1981 tggatgcgtt taatcatgaa ctgacaccgg tgaaggccag cagtatccac ccggatttga
     2041 cgttatcccg ggccgagcct caggccgggc catttgaaat tgcggctgca g
//
LOCUS       DAMZFT010000305         2036 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.308, whole genome shotgun sequence.
ACCESSION   DAMZFT010000305 DAMZFT010000000
VERSION     DAMZFT010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2036)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2036)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2036
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.308"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(548..1873)
                     /locus_tag="PN977_005293"
     CDS             complement(548..1873)
                     /locus_tag="PN977_005293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005971286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter"
                     /protein_id="HDI6045186.1"
                     /translation="MTVLQIVVLLGAIFLGVRMGGIGIGYAGGIGVLILGLCLDMKPG
                     NIPWDVILIIASVISAISAMQLAGGLDYLVQVAERILRKNPKYINYLAPVVTYVLTIF
                     AGTGHTAFSMIPVIVEVSKEQNIKPSCPLSIAVVSSQIAITASPVSAAVIYMSGVLEG
                     FGWSYPVLLGIWLFTTFVGCMLTAFIISLISDMKLDNDPVYRERLSKGLVSAPVKSVN
                     KQLKPYARRSVAIFLIGVILVVLYASAISPTLGLIDNVVVSRDAAIMSLMLLVGGFIT
                     LFCKADINKIADSSVFKSGMVACICVLGVAWLGDTFVSGHSGEIKELARTTVSQYPAL
                     LAVVFFLAAMLLYSQAATAKAITPAIVTALGITAANPDDSYMLVASFAAVSALFVLPT
                     YPTLLGAVQMDDTGTTRIGKYVFNHAFFIPGVLAIAFSVLLGFLVVSMF"
BASE COUNT          673 a          477 c          387 g          499 t
ORIGIN      
        1 tgctgcacca ctaaaaccca taaccagtgc ggcaagtgcc gttcttttat agaaattcat
       61 atctacctcc tggactgtga aatatattta ccatgtattt ttctatagaa cagttaatgc
      121 cttataaatt tgcttaataa tgatatgttt taataaattc attctcttta acttgtcatt
      181 aactcatcca gaataagatg ataaactgtt atatttcagg atataatatc cctccttgac
      241 aggtgggcga acaacattaa ctatcgataa aaaaacaccg cattccgtga taacatcatt
      301 catcaaaaat attcttatcg atgtagaaaa catcaatgta aatagaatgt ttaattacct
      361 cccattcaga atagcaatga gcgtccatca tgttttttga ttagaatcgt ataataaatg
      421 ttgcactaaa atatttataa cgaagtaaca atgcgcacct caatacttaa agattgaatg
      481 ggctaccaat caatgtggat acgtattgac gggtagccag gagattgaaa ttacgttcgg
      541 tcaagaatca gaacatactc accacaagaa atcccagaag cactgagaaa gcaatagcca
      601 gtacacccgg aataaagaaa gcatggttga acacatactt accaatacgg gtcgttcctg
      661 tgtcatccat ttgcaccgcc cccagaaggg ttgggtaagt tggtaacaca aataaagcag
      721 acacagcagc aaaagaagct accagcatgt aactgtcatc cggattcgct gcagtaatac
      781 ccaatgcagt cacaatagcc ggagtgatag ctttggcagt tgcagcctgt gaatacagaa
      841 gcatcgcagc caggaaaaat accacagcca gaagagctgg atactgggat acagtagttc
      901 tggcaagctc cttaatttct cctgaatgac cagatacaaa agtgtccccc aaccatgcca
      961 cacccagtac acagatacag gcaaccatgc ctgacttgaa cacagaggag tccgcaatct
     1021 tgtttatatc tgctttacag aaaagggtaa tgaatccgcc aaccagtagc atgagactca
     1081 taatagccgc atcacgacta acaacaacgt tatcaatcag acccagcgtc gggctaatgg
     1141 ccgaagcgta aaggaccacc aggatgacgc caataaggaa aattgcgaca gatcgtctgg
     1201 cataaggctt gagctgtttg ttaacactct ttactggcgc gcttacgagt cctttggaaa
     1261 gacgctcccg gtaaaccgga tcattgtcta atttcatgtc agaaatcaga ctgattataa
     1321 atgcggtaag catgcaacca acgaatgtgg taaacaacca tatcccaagc aatactggat
     1381 aactccaccc aaagccttcc agtacgccag acatatagat aacggcagcc gatacaggtg
     1441 atgctgtaat tgcaatttga gatgatacaa ccgcaattga gagcggacat gagggcttaa
     1501 tgttttgttc tttggaaact tcgacaatta cagggatcat tgaaaatgca gtatgtcccg
     1561 taccggcaaa gattgtcagc acataagtga caaccggagc caggtaatta atatattttg
     1621 gatttttacg aagaattcgt tcagcgacct gaacgagata gtccagtcct cccgccagtt
     1681 gcatggccga aatagcagaa attaccgatg caataattag aatcacatcc caggggatat
     1741 taccaggctt catatccaga caaagcccca gaatcaatac cccaatgcca ccggcataac
     1801 caataccaat cccgcccata cggacaccca aaaagattgc ccccagcaga accactatct
     1861 gaagaacagt gataatatcc atgattctct cccgaattag tgagacacct ttacccctga
     1921 gctcctctca acaaaggcct tatggaatgt atttttacaa acatccgggc agaggactga
     1981 tacgttttca ttcgcgccat cccctgccgg atctcccttc ttacagattc tgaaca
//
LOCUS       DAMZFT010000306         2013 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.309, whole genome shotgun sequence.
ACCESSION   DAMZFT010000306 DAMZFT010000000
VERSION     DAMZFT010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2013)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2013)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2013
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.309"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..196
                     /locus_tag="PN977_005294"
     CDS             <1..196
                     /locus_tag="PN977_005294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FHIPEP family type III secretion protein"
                     /protein_id="HDI6045187.1"
                     /translation="LAQTQEALSRQEMLGAPPVLLVNHALRPLLSRFLRRSLPQLVVL
                     SNLELSDNRHIRMTATIGGK"
     gene            196..588
                     /gene="flhe"
                     /locus_tag="PN977_005295"
     CDS             196..588
                     /gene="flhe"
                     /locus_tag="PN977_005295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlhE"
                     /protein_id="HDI6045188.1"
                     /translation="MRTLLAILLFPLLVQAAGEGMWQASSVGVTLNHRGESMSSAPLS
                     TRQPASGLMTLVAWRYQLIGPTPVGLRVRLCSQSRCVELEGQSGTTVAFSGIPAAEPL
                     RFIWEVPGGGRLIPPLKVQRNEVIVNYR"
     gene            complement(641..>2013)
                     /gene="argS"
                     /locus_tag="PN977_005296"
     CDS             complement(641..>2013)
                     /gene="argS"
                     /locus_tag="PN977_005296"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HDI6045189.1"
                     /translation="PNVAKEMHVGHLRSTIIGDAAVRTLEFLGHKVIRANHVGDWGTQ
                     FGMLIAWLEKQQQENAGEMELADLEGFYRDAKKHYDEDEEFAERARNYVVKLQSGDEY
                     FREMWRKLVDITMTQNQITYDRLNVTLTRDDVMGESLYNPMLPGIVADLKAKGLAVES
                     EGATVVFLDEFKNKEGEPMGVIIQKKDGGYLYTTTDIACAKYRYETLHADRVLYYIDS
                     RQHQHLMQAWAIVRKAGYVPESVPLEHHMFGMMLGKDGKPFKTRAGGTVKLADLLDEA
                     LERARRLVAEKNPDMPADELEKLANAVGIGAVKYADLSKNRTTDYIFDWDNMLAFEGN
                     TAPYMQYAYTRVLSVFRKAEIDEEQLAAAPVIIREDREAQLAARLLQFEETLTVVARE
                     GTPHVMCAYLYDLAGLFSGFYEHCPILSAENEEVRNSRLKLAQLTAKTLKLGLDTLGI
                     ETVERM"
BASE COUNT          414 a          540 c          535 g          524 t
ORIGIN      
        1 actggcgcaa actcaggaag cgctatcccg tcaggagatg ttgggtgcgc cgccagtttt
       61 attggtgaac cacgcgctgc gacctttatt gtctcgcttc ctgcgccgca gtctgccgca
      121 gttagtggtg ctgtcgaatc tggaactgtc tgataaccga catatccgca tgacggcgac
      181 aattggcggt aaatgatgag aaccttatta gcaatattat tgtttccgct gctggtgcaa
      241 gccgccggag aggggatgtg gcaggcgagt agtgtgggcg ttacgctgaa tcaccgtggt
      301 gagtcgatgt cgtcagcgcc tctttctact cgacaacctg cttcaggatt gatgacgctg
      361 gtggcgtggc gttatcagct tatcggccca actcccgtag ggctgcgggt tcgcttgtgt
      421 tcacaatctc gttgtgtcga attagagggg cagagcggaa ccaccgtggc cttttccggt
      481 ataccggcgg cagaaccgtt gcgctttatc tgggaagtgc ctggcggtgg gcggttaatt
      541 ccgccgctaa aggtacaacg gaatgaagtg attgtgaatt atcgttaatg ataaaaaagc
      601 cgatgtctgc catgacatcg gcttttgccg tataacgcga ttacatacgc tctactgtct
      661 caatacccag cgtatccaga cccagtttca gcgtcttcgc cgtcagttgt gccagtttca
      721 gacggctgtt acgcacttct tcgttttctg cgctgaggat cgggcagtgc tcgtagaagc
      781 cagagaacag accggccaga tcgtacaggt aagcacacat cacatgcggc gtgccttcac
      841 gggcaaccac agtgagggtt tcttcaaact gcagcaggcg agctgccagt tgtgcttcac
      901 gatcttcacg gatgattacc ggagctgcag ccagttgctc ttcgtcaatt tctgctttac
      961 ggaacacgga caatacacgc gtgtaagcat actgcatgta tggcgcggta ttaccctcaa
     1021 acgccagcat gttgtcccag tcgaagatgt agtcagtggt acggtttttg gagagatctg
     1081 cgtatttaac cgcaccaata ccaaccgcat tagccagttt ttccagctca tcggctggca
     1141 tatccgggtt cttctctgcc accagacggc gtgcgcgttc cagggcttca tccagcaggt
     1201 ctgccagttt cactgtacca cccgcgcggg ttttgaacgg tttgccgtct ttacccagca
     1261 tcatgccgaa catgtggtgt tccagcggta cggattccgg tacatagcct gctttgcgga
     1321 cgatagccca cgcctgcatc aggtgttgat gctgacggga gtcgatgtaa tacagcacgc
     1381 gatcggcatg cagtgtttca taacgatatt tcgcacaggc gatatcagtg gtggtgtaga
     1441 gatagccacc atctttcttc tgaatgatca cgcccatcgg ttcgccttcc ttgtttttga
     1501 actcatcaag gaatacgacg gtcgcgcctt cactttctac tgccagacct ttggctttca
     1561 ggtccgctac gatacccggc agcattgggt tgtagaggct ttcacccatc acgtcatcac
     1621 gggtcagcgt cacgttgaga cgatcgtagg tgatctggtt ctgcgtcatg gtgatgtcga
     1681 ccagtttgcg ccacatctcg cggaaatatt cgtcaccgct ttgcagtttt accacgtagt
     1741 tacgtgcacg ctcggcaaac tcttcatctt cgtcgtaatg ctttttcgca tcgcggtaga
     1801 aaccttcaag gtcagccagc tccatttcac cggcgttttc ctgctgctgc ttttccagcc
     1861 acgcaatcag cataccgaac tgagtgcccc agtcgccgac gtggtttgcg cgaatcactt
     1921 tgtgaccgag gaactccaga gtacgaactg ctgcgtcacc aataatggta gagcgcaggt
     1981 gaccgacatg catctctttc gccacgtttg gcg
//
LOCUS       DAMZFT010000307         2006 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.310, whole genome shotgun sequence.
ACCESSION   DAMZFT010000307 DAMZFT010000000
VERSION     DAMZFT010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2006)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2006)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2006
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.310"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..408
                     /locus_tag="PN977_005297"
     CDS             <1..408
                     /locus_tag="PN977_005297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF932 domain-containing protein"
                     /protein_id="HDI6045190.1"
                     /translation="VPHRGNVVDRVIEGAYEVVGVFDRIEEKRDAMQSLVLPPPARQA
                     LAQAALTYRYGDEHQPVTTADILTPRRREDYGKDLWSAYQTIQENMLKGGISGRSAKG
                     KRIHTRAIHSIDTDIKLNRALWVMAETMLESLR"
     gene            490..969
                     /locus_tag="PN977_005298"
     CDS             490..969
                     /locus_tag="PN977_005298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HDI6045191.1"
                     /translation="MKSVSQNTPTIYSATTPENNPPQLVASLVPDEQRISFWPQHFGL
                     IPQWVTLEPRVFGWMDRLCEDYCGGIWNLYTLNNGGAFMAPEPDDDDDETWVLFNVMN
                     GNRAEMSPEAAGIAACLMTYSHHACRTENYAMTVHYYRLRDYALQHPECSAIMRIID"
     gene            985..1461
                     /locus_tag="PN977_005299"
     CDS             985..1461
                     /locus_tag="PN977_005299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RadC family protein"
                     /protein_id="HDI6045192.1"
                     /translation="MQQLSFLPGAMTPGERSLIQRALKTLDRHLHEPGVAFTSTHAAR
                     EWLILNMAGLEREEFRVLYLNNQNQLIAGETLFTGTINRTEVHPREVIKRALYHNAAA
                     VVLAHNHPSGEVTPSKADRLITERLVQALALVDIRVPDHLIVGGNQVFSFAEHGLL"
     gene            1524..1745
                     /locus_tag="PN977_005300"
     CDS             1524..1745
                     /locus_tag="PN977_005300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF987 domain-containing protein"
                     /protein_id="HDI6045193.1"
                     /translation="MKIITRGEAMRIHQQHPASRLFPFCTGKYRWHGSTDTYTGREVQ
                     DIPGVLAVFAERRKDSFGPYVRLMSVTLN"
     gene            1825..>2006
                     /locus_tag="PN977_005301"
     CDS             1825..>2006
                     /locus_tag="PN977_005301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system YeeU family
                     antitoxin"
                     /protein_id="HDI6045194.1"
                     /translation="MSDTHPGTTHPDNNNDRPWWGLPCTVTPCFGARLVQEGNQLHYL
                     ADRAGIRGQFSDADAY"
BASE COUNT          454 a          560 c          546 g          446 t
ORIGIN      
        1 gttccgcacc ggggaaatgt ggtggacaga gtcattgaag gggcttacga agtggtgggc
       61 gtgtttgacc ggatagagga gaagcgtgat gccatgcagt cgctggtcct gccgccaccg
      121 gcacgccagg cgctggcaca ggcggcactg acttaccgtt atggtgacga acatcagccc
      181 gtcaccaccg ccgacattct gacgccgcga cgccgggagg attacggtaa ggacctgtgg
      241 agcgcatatc agaccatcca ggagaatatg ctgaaaggcg ggatttccgg tcgcagtgca
      301 aaaggaaaac gtatccatac ccgggccatt cacagcatcg acaccgatat taaactcaac
      361 cgcgcattgt gggtgatggc agaaacgatg ctggagagcc tgcgctgatg ccgtttccct
      421 gagtgaggga atcattcagc tttcgttcct gaaaaacaca cttttatttg ttatttaagg
      481 agttatctca tgaaaagcgt ttctcagaat acccccacaa tttattcagc tacaacacca
      541 gagaataatc cgcctcagtt ggttgccagc ctcgtccctg atgaacagcg catcagcttt
      601 tggccgcagc attttggcct cattccacag tgggtgaccc tggagccccg tgtcttcggc
      661 tggatggacc gtctgtgtga agactactgc ggtggtatct ggaatctgta caccctgaac
      721 aacggtggcg catttatggc acccgaaccg gatgacgatg atgacgaaac atgggtgctg
      781 tttaacgtca tgaacggtaa ccgcgctgaa atgagcccgg aagccgccgg cattgccgcc
      841 tgtctgatga cgtacagtca tcacgcctgt cgtacggaga attatgccat gacggtccat
      901 tattatcggt tgcgggatta cgccctgcag catccggaat gcagcgccat tatgcgcatc
      961 atcgactgaa tggagagaag cataatgcaa cagctttcct ttctgcccgg agcgatgacg
     1021 cccggcgagc gcagcctcat tcagcgggcc ctgaaaaccc tggaccgtca tcttcatgag
     1081 cccggcgtgg ccttcacctc cacgcatgcg gcacgggaat ggctgattct gaacatggca
     1141 ggactggagc gggaagagtt ccgggtgctg tatctgaaca accagaatca gctgattgcg
     1201 ggtgaaaccc tcttcacagg caccatcaac cgcacagaag tccatccccg ggaggtgatt
     1261 aaacgtgccc tgtaccacaa tgccgctgcc gtggtgctgg cgcacaatca cccgtccggt
     1321 gaagtcaccc ccagtaaggc agaccggctt atcactgaac gtctggtaca ggcgctggcc
     1381 ctggtggata tccgggtgcc ggaccatctg atagtcggtg gcaaccaggt attctccttt
     1441 gccgaacacg gtctgcttta acccgtcaca accacatcac acctgttttc acttttatct
     1501 tctgtcttca gaggtatccc attatgaaaa tcatcacccg tggtgaagcc atgcgtattc
     1561 accaacagca tcctgcatcc cgtctttttc cgttctgtac cggtaaatac cgctggcacg
     1621 gtagcacgga tacgtatacc ggccgtgaag tgcaggatat tcccggtgtg ctggccgtgt
     1681 ttgctgaacg ccgtaaggac agttttggtc cgtatgtccg tctgatgagc gttaccctga
     1741 actgaatcag aaagggcatt ctgatgagcc gaattatcgc caccatcgga gcattcttaa
     1801 ccgattttct gtgaggattt tatcgtgtca gacacacacc ccgggacaac gcatcccgac
     1861 aacaacaacg accgcccctg gtgggggctc ccctgcaccg tgacgccctg tttcggggca
     1921 cgtctggtgc aggagggcaa tcagttgcat taccttgccg accgcgccgg tatcaggggg
     1981 cagtttagcg acgcggatgc gtatca
//
LOCUS       DAMZFT010000308         1986 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.311, whole genome shotgun sequence.
ACCESSION   DAMZFT010000308 DAMZFT010000000
VERSION     DAMZFT010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1986)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1986)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1986
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.311"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            106..>1986
                     /locus_tag="PN977_005302"
     CDS             106..>1986
                     /locus_tag="PN977_005302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3-like element Tn5403 family transposase"
                     /protein_id="HDI6045195.1"
                     /translation="MSRRHIFTERQRAALFDLPTDELSLLKFYTLGDDDLENIRQRRR
                     PENRIGFALQLCALRYPGRALAPGEMIPREILSFVGAQLGVPADALLTYATRRQTRQQ
                     HMDTLREIYGYKTFTGRGARDLREWTFGQAEDARSNEDLAHRFIVRCRETSTILPAVS
                     TIERLCADALVAAERRIETRIVENLTADVRDHLDKLLSEMLAGNISRFIWLRNFEVGN
                     NSAAANRLLDRLEFLRTLNINHSALASIPAHRIARLRRQGERYFTDGLRDITSDRRWA
                     ILAVCVVEWEAAIADAIVETHDRIVGKTWREAKRQHDETISGSKATLADTIRTFTALG
                     ASLLEARSDGTPLEMAVASSVAWDRLAQLVATGTQLSNTLADEPLAYVGQGYHRFRRY
                     APRMLRCLKLEAAPVAGPLVAAALSIGEMKGVASPERRFLRPSSKWNRHLRAQEKGDT
                     RLWEVAVLFHLRDAFRSGDVWLAHSRRYGDLKQVLVPMIAAQENAKLAVPSNPQDWLA
                     DRKARLTIALKRLARAARNGTIPHGSIEDGTLRIDRLTADVPDGAEALILDLYRRMPS
                     VRITDMLLEVDAALGFTDAFTHLRTGAPCRDRIGLLNVLLAEGLNLGLRKMAEATNTH
                     DYW"
BASE COUNT          420 a          550 c          568 g          448 t
ORIGIN      
        1 cgatgtccca attgaaccct cctggatttt tgtatcggaa cgccctgatt tatatgggct
       61 ggctgttgtc caaaacagac tatacttcaa aagggacgaa tttgtatgtc acgacgccat
      121 attttcaccg aacggcagcg agcagcgctg ttcgatctgc ccacggacga actgtcgcta
      181 ctgaagttct acacgctggg cgatgatgac ctggaaaaca ttaggcagcg ccgcagaccg
      241 gaaaacagga ttggctttgc cctgcaactt tgtgccttac gatatccggg ccgtgcactg
      301 gctcctggtg agatgatccc gcgtgaaatc ctttccttcg tcggtgctca gcttggagtt
      361 ccggctgatg cgcttctcac ttatgccaca cggcgccaaa cccgtcagca gcacatggac
      421 acgctgcgcg aaatttacgg ctacaagacc ttcacgggcc gtggtgcccg tgatctgcgg
      481 gagtggactt tcggccaggc cgaagatgcc agatcaaacg aggatcttgc tcatcgtttt
      541 attgtgcggt gtcgggaaac ttccaccatt ctgcccgcag tatcgacaat cgagcgcttg
      601 tgcgcggatg ctctggtcgc cgctgagcgg cggattgaaa cgcggattgt ggaaaattta
      661 acagcggatg ttcgcgatca cctggacaaa cttctgagtg aaatgctcgc cggcaatatc
      721 agtcgtttca tctggcttcg caacttcgag gttggtaaca actcggctgc tgctaaccgt
      781 ttgctcgaca ggctcgaatt tctgcgtacc ctgaatatca atcatagtgc tttggccagc
      841 atacctgccc atcgcattgc ccggctgcgt cggcagggtg aacgctactt caccgacggt
      901 ttgcgtgaca tcacttcgga ccgccgctgg gcgatccttg ccgtctgtgt tgtggagtgg
      961 gaagcggcga ttgctgatgc catagtcgaa acccatgaca ggatcgtagg aaaaacctgg
     1021 cgggaagcga agcgccagca tgacgaaaca atttccggct ctaaagccac actcgcggat
     1081 acgatccgta ccttcaccgc gctgggagct tcgttgcttg aggcccgcag tgacggaacc
     1141 ccgctggaga tggctgtcgc cagttcggtt gcatgggacc ggctcgctca actggtagcg
     1201 acagggactc aactcagcaa cacgctagcc gatgagcctc ttgcatatgt cgggcaggga
     1261 taccatcgct ttcgtcgtta tgcgccccgc atgttgcgct gtctgaagct cgaagccgcg
     1321 ccggtcgccg gaccattggt agcagcagct ttgtcgatcg gagagatgaa aggtgttgca
     1381 tcgccagaaa ggcgtttcct gcggcccagc tccaaatgga accgtcattt acgagctcag
     1441 gaaaaaggag atacccgtct ttgggaagtg gcggtactct ttcacctccg ggatgctttt
     1501 cgttccggag atgtctggct cgctcattcg cgccgctatg gtgacctcaa gcaggtactg
     1561 gtgccgatga tcgcggcgca ggaaaatgca aaactggccg tgccttccaa cccacaggat
     1621 tggctggcag acagaaaggc gcgactcacg atcgctctta agcggctggc ccgggctgcc
     1681 cgtaacggca ctattccgca cggtagcata gaagatggaa cgttgcggat cgacaggttg
     1741 acagcagacg tgccggatgg tgccgaggca ctcatactgg atctgtatcg ccgaatgccg
     1801 tccgttcgga ttaccgacat gctgcttgaa gttgatgcag cccttggttt cacagatgcg
     1861 tttacccatc tgagaaccgg ggctccatgt cgcgaccgga tcggtctgct caacgtcctg
     1921 ctcgctgaag ggctcaatct gggcctgcgt aagatggcgg aagctacaaa cacgcatgat
     1981 tactgg
//
LOCUS       DAMZFT010000309         1970 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.312, whole genome shotgun sequence.
ACCESSION   DAMZFT010000309 DAMZFT010000000
VERSION     DAMZFT010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1970)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1970)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1970
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.312"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..1161
                     /gene="araA"
                     /locus_tag="PN977_005303"
     CDS             <1..1161
                     /gene="araA"
                     /locus_tag="PN977_005303"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HDI6045196.1"
                     /translation="SIDMDFMNLNQTAHGGREFGFIGARMRQQHAVVTGHWQDKQAHE
                     RIGSWMRQAVSKQDTRHLKVCRFGDNMREVAVTDGDKVAAQIKFGFSVNTWAVGDLVQ
                     VVNSISDGDVNALVDEYESCYTMTPATQIHGEKRQNVLEAARIELGMKRFLEQGGFHA
                     FTTTFEDLHGLKQLPGLAVQRLMQQGYGFAGEGDWKTAALLRIMKVMSTGLQGGTSFM
                     EDYTYHFEKGNDLVLGSHMLEVCPSIAVEEKPILDVQHLGIGGKDDPARLIFNTQTGP
                     AIVASLIDLGDRYRLLVNCIDTVKTPHSLPKLPVANALWKAQPDLPTASEAWILAGGA
                     HHTVFSHALNLNDMRQFAEMHDIEITVIDNDTRLPAFKDALRWNEVYYGFRR"
     gene            1361..>1970
                     /gene="araD"
                     /locus_tag="PN977_005304"
     CDS             1361..>1970
                     /gene="araD"
                     /locus_tag="PN977_005304"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HDI6045197.1"
                     /translation="MLEDLKRQILEANLALPKHNLVTLTWGNVSAVDRERGVLVIKPS
                     GVDYNVMTADDMVVVSIETGEVVEGTKKPSSDTPTHRLLYQAFPSIGGIVHTHSRHAT
                     IWAQAGQSIPATGTTHADYFYGTIPCTRKMTDAEINGEYEWETGNVIVETFEKQGIDA
                     AQMPGVLVHSHGPFAWGKNAEDAVHNAIVLEEVAYMGIFCRQL"
BASE COUNT          470 a          555 c          537 g          408 t
ORIGIN      
        1 agcatcgaca tggactttat gaacctgaac cagaccgcgc atggcggtcg cgagttcggc
       61 ttcatcggcg cgcgtatgcg tcagcaacat gccgtcgtta ccggtcactg gcaggataaa
      121 caagcacatg agcgtatcgg ctcctggatg cgtcaggcgg tatctaaaca ggatacccgt
      181 catctgaaag tctgccgttt tggcgataac atgcgtgaag tggcggtcac cgatggcgat
      241 aaagttgccg cacagatcaa gttcggtttc tccgtcaata cctgggcggt tggcgatctg
      301 gtgcaggtag tgaactccat cagcgatggc gatgttaacg cgctggtcga tgagtacgaa
      361 agctgctaca ccatgacgcc tgcgacacaa atccacggcg aaaaacgaca gaacgtgctg
      421 gaagcggcac gtattgagct ggggatgaag cgtttcctgg aacaaggtgg cttccacgcg
      481 ttcaccacca cctttgaaga tttgcacggt ctgaagcagc ttcctggtct ggccgtacag
      541 cgtctgatgc agcagggcta cggctttgcg ggcgaaggcg actggaaaac tgccgccctg
      601 cttcgcatca tgaaggtgat gtcaaccggt ctgcagggcg gcacctcctt tatggaggac
      661 tacacttacc acttcgaaaa aggtaatgac ctggtgctcg gctcccatat gctggaagtc
      721 tgtccgtcga tcgccgtaga agagaaaccg atcctcgacg ttcaacacct cggtattggc
      781 ggtaaagacg atcctgcccg cctgatcttc aacactcaaa ccggtccggc cattgtcgcc
      841 agtctgattg atctcggcga tcgttaccgt ctgctggtta actgcatcga cactgtgaaa
      901 acaccgcact ccctgccgaa actgccggtg gcgaatgcgc tgtggaaagc gcaaccggat
      961 ctgccaactg cttccgaagc gtggatcctc gctggtggcg cgcaccatac cgttttcagc
     1021 catgcgctga acctcaacga tatgcgccag ttcgccgaga tgcacgacat tgaaatcaca
     1081 gtgattgata acgatacccg cctgccagcg tttaaagacg cgctgcgctg gaacgaagtg
     1141 tattacggat ttcgtcgcta agtagccgca tcaggtatgt aacgcctgat gcgacgctaa
     1201 cgcgtcttat caggcctaca cgctgcgatt ttgtaggccg gataagcaaa gcgcatccgg
     1261 cattcaccgc ctgatgcgac gctgacgcgt cttatcaggc ctacacgctg cgattttgta
     1321 ggccggataa gcaaagcgca tccggcacga aggagtcaac atgttagaag atctcaaacg
     1381 ccagatatta gaagccaacc tggcgctgcc aaaacataac ctggtcacgc tcacatgggg
     1441 caacgtcagc gccgttgatc gcgagcgcgg cgtcctggtg atcaaacctt ccggcgtcga
     1501 ttacaacgtc atgaccgctg acgatatggt cgtggtcagc atcgaaaccg gtgaagtggt
     1561 tgaaggaacg aaaaagccct cctccgacac gccaactcac cggctgctct atcaggcatt
     1621 tccgtccatt ggcggcattg tgcacacaca ctcgcgccac gccaccatct gggcgcaggc
     1681 gggtcagtcg attccagcaa ccggtaccac ccacgccgac tatttctacg gcaccattcc
     1741 ctgcacccga aaaatgaccg acgcagaaat caacggtgaa tatgagtggg aaaccggtaa
     1801 cgtcatcgta gaaaccttcg aaaaacaggg tatcgatgcg gcgcaaatgc ccggcgtcct
     1861 ggtccattct cacggcccat ttgcatgggg caaaaatgcc gaagatgcag tgcataacgc
     1921 catcgtgctg gaagaggtcg cttatatggg gatattctgc cgtcagttag
//
LOCUS       DAMZFT010000310         1968 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.313, whole genome shotgun sequence.
ACCESSION   DAMZFT010000310 DAMZFT010000000
VERSION     DAMZFT010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1968)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1968)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1968
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.313"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(365..754)
                     /locus_tag="PN977_005305"
     CDS             complement(365..754)
                     /locus_tag="PN977_005305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDI6045198.1"
                     /translation="MRELINTIAHIGYQVSDLTRSLAFYEPLGFQRQQRFSKPSPQGT
                     IEVAFLEMAGAVLELYQLPAGTPFDAPRCGIDHLALEVSDLDAVQQRLAALGYQLDEG
                     PIVEDNVRFLLIRGPDGERLEFDACKR"
     gene            complement(756..>1968)
                     /gene="fucP"
                     /locus_tag="PN977_005306"
     CDS             complement(756..>1968)
                     /gene="fucP"
                     /locus_tag="PN977_005306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009654976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="L-fucose:H+ symporter permease"
                     /protein_id="HDI6045199.1"
                     /translation="LWGMAGNLNDILIAQFKKGFDLTDTQTALVQSIFFLGYFFVALP
                     AAALIKRFSYKAAIIIGLCLYALGCFLFVPAAQIMTYGAFLACLGVIACGLSFLETSA
                     NTYSSLLGPIQSSTQRINFSQIFNSLGVISGVLIGQLMVFGENDPSHEQLLAMPAAAA
                     DVARHQMVGQVVGPYLIIGSVLVVLALVFVFIKFPSCKGAPAQQQQLPTESMGPTLKR
                     LFAIPRFRLGILSQFLYVGAQVGVWSFTIRFVQLVQQGTSEHSATYWLLASLVIYAVG
                     KTVATWLMNRLNPAMLLGTFALAATALLLIAVFSGSMLAVYALILVSFCMAPCWPTNF
                     GLVIKGMGKDTQTAGSIVVMSIIGGAVIPLVMGIISDMNGGNMQIAFIAPLLCFVYVA
                     FYGFWCVRKGV"
BASE COUNT          510 a          540 c          564 g          354 t
ORIGIN      
        1 atcgcggtac agatagcgcc gatcctcaaa cgccagcgcc agggcatccg ggctgcgggc
       61 ggcgctgtgg ctcagggcct ggtacagatt ctggaagtcg gcggaggtgc tgttcatgtg
      121 tctctcctga tatcgcgtat tgagtaggag tagtctgggg aaattcagga gaggaaattg
      181 ccgaatggct cacaatagaa aatcaacaaa acagaagcaa ttttgtaaca ttgcacaatg
      241 ggcaggagag accgggtggg ggagagatat ccgcgaatca aaccgacgtg aataaagcca
      301 gaggacgtat cttttagacc cgtgttaacc gggcctaaaa gagtgaaggg gtcagccctg
      361 ccgcttagcg tttacaggca tcgaactcga gccgttcgcc atcggggccg cgaataagca
      421 ggaagcgcac gttgtcttct actatcggcc cttcatcgag ctgataaccg agcgccgcca
      481 gccgctgctg caccgcatcc aggtcgctga cttccagcgc cagatgatcg ataccgcagc
      541 gaggggcgtc aaagggcgtt ccagcgggaa gctgatacag ctcgaggacg gcgcccgcca
      601 tttccaggaa cgcaacttca atagtgccct gtggcgatgg cttgctaaac cgctgctggc
      661 gctgaaagcc cagcggttca tagaaggcaa gcgaacgcgt taagtcactg acctgatagc
      721 caatatgagc aatcgtgttg ataagttcgc gcatgttata cccccttacg cacgcaccag
      781 aagccgtaga aagcgacata cacaaagcac aggagcggag cgataaaggc gatttgcata
      841 ttgccgccgt tcatatccga gataatgccc atcaccagag gaattacggc cccgccgata
      901 atcgacatca ccacgatcga gcctgcggtc tgggtatctt tccccatccc tttgatcacc
      961 agaccaaagt tcgtcggcca gcatggcgcc atacagaagc tgaccagaat gagcgcatag
     1021 accgccagca ttgaaccgct gaaaacggca atcagtaaca gggcggtggc ggccagggca
     1081 aacgttccga gcagcatcgc cggattcaga cggttcatca accaggtggc cacggttttt
     1141 cccacggcgt aaatcaccag agaagccagc agccagtaag tcgcggagtg ctcgctggtg
     1201 ccttgctgca cgagctgcac aaagcgaatc gtgaagctcc atacgccaac ctgggcgccg
     1261 acgtacaaaa actgcgacag gatcccgagg cgaaagcgcg ggatagcaaa caggcgtttc
     1321 agcgttggcc ccatgctttc cgtcgggagt tgctgttgtt gagcgggcgc gcccttgcac
     1381 gacgggaatt taataaacac gaacaccagc gccagcacca ccagtacgga gccgataatc
     1441 agatagggcc cgaccacctg accaaccatc tgatggcgtg caacgtctgc agcggcggcg
     1501 ggcatcgcca gcagttgttc atgactcgga tcgttttcgc caaagaccat cagctggcca
     1561 attaatacgc cggagatcac gcccagcgag ttgaagatct gcgaaaagtt aatacgctgg
     1621 gtggaggatt gaatcggacc cagcaggctg gagtaggtgt ttgccgacgt ttccagaaaa
     1681 gagagtccgc aggcgatcac tccgaggcag gcgaggaatg cgccgtaggt catgatctgt
     1741 gcggccggaa cgaacaggaa gcagccgagg gcgtacaggc acagcccaat aataatcgcc
     1801 gctttatagg aaaaacgctt aatcagcgcc gccgcgggca gggcgacaaa aaagtagccg
     1861 aggaaaaaaa tcgactgcac cagcgctgtc tgggtatccg ttaaatcgaa gcccttttta
     1921 aactgggcga tcaaaatatc attcaggttg cccgccatcc cccatagc
//
LOCUS       DAMZFT010000311         1927 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.314, whole genome shotgun sequence.
ACCESSION   DAMZFT010000311 DAMZFT010000000
VERSION     DAMZFT010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1927)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1927)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1927
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.314"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..354
                     /locus_tag="PN977_005307"
     CDS             <1..354
                     /locus_tag="PN977_005307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HDI6045200.1"
                     /translation="ENMLDQAFKKLNPHEHPVLHSDQGWQYRMRKYQNILKEHGIKQS
                     MSRKGNCLDNAVVECFFGTLKSECFYLDEFSNISELKDAVTEYIEYYNSRRISLKLKS
                     LTPIEYRNQTYMPRV"
     gene            complement(897..1499)
                     /locus_tag="PN977_005308"
     CDS             complement(897..1499)
                     /locus_tag="PN977_005308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000813428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6045201.1"
                     /translation="MKQQYQTRYELLHENYQKWLTGFTRHAVSWGVCHPNIYYFHNLT
                     PGWVSFNGEKPEIAIVPQSLHRLIYGPDKRSSPSLDDDLVVNLCTSEHLLVHHPMLEG
                     ILLSECERLKQHSLANKLISLFRQFGGTELRLKLVWLCWLDLMTGNSLDDWTKNLKHK
                     SEKDLEQWIIARQGQSEPLTNLMDQYVLMAYRTSVDAAHS"
     gene            complement(1593..1799)
                     /locus_tag="PN977_005309"
     CDS             complement(1593..1799)
                     /locus_tag="PN977_005309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020234041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family phage regulatory protein"
                     /protein_id="HDI6045202.1"
                     /translation="MATPVSLMDDQMVDMAFITQLTGLTDKWFDKLIKDGGFPAPIKM
                     GRSSRWLKSEVEAWLQARIAQSRP"
BASE COUNT          488 a          450 c          494 g          495 t
ORIGIN      
        1 gagaatatgc tcgatcaggc attcaaaaag cttaatcctc acgagcatcc tgttctgcac
       61 tctgaccagg gatggcagta tcgtatgaga aaatatcaaa atattcttaa agaacatggt
      121 attaaacaaa gcatgtccag aaaaggcaat tgtctggata atgctgtggt ggagtgtttc
      181 tttggaacct taaagtcgga gtgtttttat cttgatgagt tcagtaatat aagcgaactg
      241 aaggatgctg ttacggaata tattgaatac tacaacagca gaagaattag cctgaaatta
      301 aaaagtctga ctccaattga atatcggaat cagacctata tgcctcgtgt ttaactgtcc
      361 aactttttgg ggtcagtaca gaatgaggct tgccatcagc cgaaacatca gtattccacg
      421 ccatacagac ggcgcctgac catgtgctga tgcaggtaaa atgtttcact acaactgttc
      481 gggttgctat gcaacgaaac agcgattctc acgatcttta actgcaacgt ttttccgcct
      541 gaaagctgac tgcctcctgc tgaggtggga ggctatgatt cagtgcgttt atagtctccg
      601 gggtatgcat gtaccggagc ataccatttc tggtggataa caagccggca cattcagact
      661 gcagtgaaga tggaggcacc tggttgtgaa gaacgtttct gcacgtggct gcttcgcatc
      721 atcaatggta ttcatacccg ctcagaaatg accgggtgct gaagccctcc gttatcgcgc
      781 agtataatga cgtatatgat ctgtggacga gtgagcggcg tgcctgatgt acgccgctat
      841 tactgtattt taccacaggg ctggttttct gctgccagcc ctgtggttct cagacatcaa
      901 ctgtgagcgg catcaaccga tgtacggtat gccataagca cgtactgatc catcagattt
      961 gtcagtggtt cactctgacc ctgacgtgca atgatccatt gctccagatc tttttctgat
     1021 ttgtgtttca ggtttttcgt ccagtcgtca aggctgtttc cggtcattaa gtcaagccag
     1081 cagagccaga ccagtttgag acgtaactcc gtgccgccaa actgacggaa gaggctgatc
     1141 agtttgtttg ccagagaatg ttgcttcagg cgctcacatt cagacagcag gatcccttcc
     1201 agcatcggat gatgaaccag caggtgttcg ctggtgcata aattcaccac cagatcatca
     1261 tccagggacg gggagctccg tttgtccgga ccataaatca gccggtgcag actctgggga
     1321 acgatggcaa tctccggttt ttcgccattg aatgacaccc acccgggcgt cagattatgg
     1381 aaatagtaga tattcggatg acacacgccc catgatacgg cgtgccgggt gaagccggtc
     1441 agccatttct ggtagttttc atggagcagc tcgtaacggg tctgatactg ttgtttcatt
     1501 ttcatattcc tcacggggga taacaggcag cagagcgccg ccggcaggcc ggagattgcg
     1561 tctgctgtgg gcgaagggtt agtggttaaa aattacggac gggactgtgc aatacgcgcc
     1621 tgcagccagg cttccacttc acttttcagc cagcgggagc tgcgccccat tttgatgggc
     1681 gcaggaaagc ccccatcttt gatgagtttg tcaaaccact tatcggttag gccggtcagt
     1741 tgagtgataa acgccatgtc gaccatctgg tcatccatca gcgaaactgg ggtagccatg
     1801 agaatgttcc tccggatgtt gttaaaccag ccggtgtggt catgaaaaca gcgcgccacc
     1861 ttcaccggca taacaaccgg ggagaaacgt aaaaaataat cgggaatgtg gctgtccgga
     1921 gataaac
//
LOCUS       DAMZFT010000312         1901 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.315, whole genome shotgun sequence.
ACCESSION   DAMZFT010000312 DAMZFT010000000
VERSION     DAMZFT010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1901)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1901)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1901
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.315"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(3..1055)
                     /gene="ompA"
                     /locus_tag="PN977_005310"
     CDS             complement(3..1055)
                     /gene="ompA"
                     /locus_tag="PN977_005310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706879.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpA"
                     /protein_id="HDI6045203.1"
                     /translation="MKKTAIAIAVALAGFATVAQAAPKDNTWYTGAKLGWSQYHDTGF
                     IPNNGPTHENQLGAGAFGGYQVNPYVGFEMGYDWLGRMPYKGDNINGAYKAQGVQLTA
                     KLGYPITDDLDVYTRLGGMVWRADTKSNVPGGASTKDHDTGVSPVFAGGVEYAITPEI
                     ATRLEYQWTNNIGDAHTIGTRPDNGMLSLGVSYRFGQGEAAPVVAPAPAPAPEVQTKH
                     FTLKSDVLFNFNKATLKPEGQAALDQLYSQLSNLDPKDGSVVVLGYTDRIGSDAYNQA
                     LSERRAQSVVDYLISKGIPADKISARGMGESNPVTGNTCDNVKQRAALIDCLAPDRRV
                     EIEVKGIKDVVTQPQA"
     gene            complement(1411..1863)
                     /gene="sulA"
                     /locus_tag="PN977_005311"
     CDS             complement(1411..1863)
                     /gene="sulA"
                     /locus_tag="PN977_005311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017899460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibitor SulA"
                     /protein_id="HDI6045204.1"
                     /translation="MARVSTENTTAGLISEVVYREDQPMMTQLLLLPLLQQLGQQSRW
                     QLWLTPQQKLSREWVQASGLPLTKVMQISQLSPCHTVESMVRALRTGNYSVVIGWLAD
                     DLTEEEHAELVDAANEGNAMGFIMRPVSASSHATRQLSGLKIHSNLYH"
BASE COUNT          480 a          475 c          477 g          469 t
ORIGIN      
        1 acttaagcct gcggctgagt tacaacgtct ttgatacctt taacttcgat ctctacgcga
       61 cgatccggag ccaggcagtc gatcagtgca gcacgctgtt tcacgttgtc acaggtgttg
      121 ccagtaaccg ggttggattc gcccatacca cgtgcggaga ttttgtctgc cgggatacct
      181 ttagagatca ggtaatcaac aacggactga gcacgacgct cggacagagc ctggttgtaa
      241 gcgtcagaac cgatgcggtc ggtgtaaccc agaacaacta cggaaccgtc tttcggatcc
      301 aggttgctca gctggctgta cagctgatcc agagcagcct gaccttccgg tttcagggtt
      361 gctttgttga agttgaacag aacgtcagac ttcagagtga agtgcttggt ctgtacttcc
      421 ggtgccggag ctggagccgg agcaactact ggagctgctt cgccctgacc gaaacggtag
      481 gaaacaccca ggctcagcat gccgttgtcc ggacgagtac cgatggtgtg tgcgtcaccg
      541 atgttgttgg tccactggta ttccagacgg gtagcgattt caggagtgat cgcgtactca
      601 acaccgccag cgaagaccgg agaaacgccg gtgtcgtggt ctttagtgga tgcgccacca
      661 ggtacgttag acttggtgtc tgcacgccat accataccac ccagacgagt gtaaacgtcc
      721 agatcgtcag tgattgggta acccagttta gcggtcagct gaacgccctg agctttgtat
      781 gcgccgttga tgttgtcgcc tttgtacggc atacgaccta accagtcgta acccatttca
      841 aagccaacat acgggttaac ctggtaacca ccaaaagcac ctgcacccag ttggttttcg
      901 tgggtcggac cattgttagg aataaaacca gtgtcatggt actgggacca gcccagttta
      961 gcaccagtgt accaggtgtt atctttcgga gcggcctgcg ctacggtagc gaaaccagcc
     1021 agtgccactg caatcgcgat agctgtcttt ttcatttttt gcgcctcgtt atcatccaaa
     1081 atacgccatg aatatctcca acgagataac acggttaaat ccttcaccgg ggatctgctc
     1141 aatattaact ctaccgatat cttcggctta tgccgagcac ccctggcgat gtaaagtcta
     1201 caacgtagtt gaaaacttac aagtgtgaac tccgtcaggc atatgaaaaa aaagtcttgt
     1261 ataaggtacg tttaatcttt tttgtcagcg acaatttaca gaagaaaatc gcggaaaccg
     1321 cttcagacaa gcctctgcaa ggaaaattag tcacgactga aagcattggc tgggcgacaa
     1381 aaaaagttcc aggattaatc ctaaatttac ttaatgatac aaattagagt gaatttttag
     1441 cccggaaagt tgtctcgttg cgtgagagga agcgcttacc ggacgcataa taaaccccat
     1501 agcgttacct tcatttgccg catcaacaag ttcagcatgc tcttcttcag tcaaatcatc
     1561 tgccaaccaa ccgatcacca cactgtaatt gcccgtgcgt aaagcgcgaa ccattgactc
     1621 cacggtgtgg caaggggaga gctggctaat ctgcattact ttcgttaagg gtagcccaga
     1681 tgcctgaacc cattcgcgac tcagtttttg ttgcggtgtt aaccagagtt gccagcgcga
     1741 ttgctgaccg agttgctgta acaatggcaa cagtagaagt tgcgtcatca tgggctgatc
     1801 ttcgcgatag acaacttcac tgataagccc ggctgtagtg ttttccgtag agacacgcgc
     1861 aattttactt gctgcggatg agaacgacga agaacgatgt g
//
LOCUS       DAMZFT010000313         1867 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.316, whole genome shotgun sequence.
ACCESSION   DAMZFT010000313 DAMZFT010000000
VERSION     DAMZFT010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1867)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1867)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1867
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.316"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..1458
                     /locus_tag="PN977_005312"
     CDS             <1..1458
                     /locus_tag="PN977_005312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HDI6045205.1"
                     /translation="RYPGVVALDNVNFTLNKGEVRALLGKNGAGKSTLIRMLTGSERP
                     DSGDIWIGETRLEGDETTLTRRAAELGVRAVYQELSLVEGLTVAENLCLGQWPRRNGM
                     IDYLQMAQDAQRCLQALGVDVSPEQLVSTLSPAQKQLVEIARVMKGEPRVVILDEPTS
                     SLASAEVELVISAVKKMSALGVAVIYVSHRMEEIRRIASCATVMRDGQVAGDVMLENT
                     STHHIVSLMLGCDHVDIAPVAPQEIMDQAVLEVRALRHKPKLEDISFTLRRGEVLGIA
                     GLLGAGRSELLKAIVGLETYEQGEIVINGEKITCPDYGDMLKRGIGYTPENRKEAGII
                     PWLGVDENTVLTNRQKISANGVLQWSTIRRLTEEVMQRMTVKAASSETPIGTLSGGNQ
                     QKVVIGRWVYAASQILLLDEPTRGVDIEAKQQIYRIVRELAAEGKSVVFISSEVEELP
                     LVCDRILLLQHGTFSQEFHSPVNVDELMSAILSVH"
     gene            1483..>1867
                     /locus_tag="PN977_005313"
     CDS             1483..>1867
                     /locus_tag="PN977_005313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDI6045206.1"
                     /translation="MSASSLPLPQGKSVSLKQFVSRHINEIGLLVVIAILYLVFSLNA
                     PGFISLNNQMNVLRDAATIGIAAWAMTLIIISGEIDVSVGPMVAFVSVCLAFLLQFDV
                     PLAIACLLVLLLGALMGTLAGVLRGV"
BASE COUNT          395 a          455 c          575 g          442 t
ORIGIN      
        1 cgttatcccg gcgtcgttgc gttggataac gttaacttca cgctcaataa aggcgaagtt
       61 cgtgcgctgt taggcaaaaa cggcgcgggc aaatcgaccc tcattcgaat gcttaccggt
      121 agcgaacgtc cggatagcgg tgatatctgg attggcgaga cgcgactgga aggtgacgaa
      181 actacgctga ctcgccgtgc cgctgaactg ggggttcgtg cggtttatca ggaattaagt
      241 ctggtggaag ggctgacggt ggcggaaaac ctctgcctcg gtcagtggcc ccgccgcaac
      301 ggcatgattg attacctgca aatggcgcag gatgcccaac gttgcttaca ggcgctgggc
      361 gttgacgtta gccctgaaca acttgtttca acgctaagcc cggcgcaaaa gcaactggtg
      421 gaaattgcgc gggtgatgaa gggcgagccg cgcgtggtca ttcttgatga acccaccagc
      481 tcgcttgcca gtgcggaagt tgaactggtg atcagcgcgg tgaaaaagat gtcggcactg
      541 ggcgtggcgg tgatttatgt cagccaccgg atggaagaaa ttcgccgcat tgcctcctgt
      601 gccaccgtta tgcgcgatgg tcaggtggcg ggcgatgtga tgctcgaaaa cacctctacg
      661 catcatattg tgtcgctaat gctcgggtgc gatcacgttg atattgcgcc ggttgcccct
      721 caggaaatta tggatcaggc cgtgctggaa gtccgtgcgt tacgccataa gcccaagctg
      781 gaggatatca gctttacgct acgtcgcggc gaagtgctcg gcattgctgg cctgctgggg
      841 gcagggcgca gtgaattgtt gaaagccata gttgggctgg agacgtatga acagggcgaa
      901 attgttatca acggcgagaa aatcacgtgc cccgattacg gcgacatgct gaaacgcggc
      961 attggatata cgccagaaaa ccgcaaagaa gcggggatca ttccctggct gggcgttgac
     1021 gaaaatacag tgctgaccaa tcggcaaaaa atcagcgcca acggtgtgct gcaatggtcc
     1081 accatccgcc gcctgaccga agaggtgatg cagcggatga cggtcaaggc cgccagtagc
     1141 gaaacgccca tcggcacgct ttctggcggc aatcagcaaa aagtggtgat cggtcgttgg
     1201 gtctatgccg ccagccagat tttgttgctc gacgagccaa cgcgtggcgt cgatatcgaa
     1261 gccaaacagc agatttaccg tattgtccgc gagctggctg ccgaaggaaa aagcgtggtg
     1321 tttatctcca gtgaagtgga ggagttaccg ttggtgtgcg accgcattct gttgttacag
     1381 cacggtacgt tctcgcagga gtttcactct ccggtcaatg tggatgagct gatgtccgcc
     1441 attctgtctg tgcactgatg aattttaccg aggaacctga agatgtctgc ttcgtcatta
     1501 ccattgccgc agggcaagag cgtctcgctc aaacaatttg tcagtcgcca tattaatgag
     1561 atcggtttgc tggtggtgat tgccattttg tatctggtct tctccctgaa cgcgccgggt
     1621 tttatctcat tgaataacca gatgaacgtg ctgcgtgatg ccgccaccat tgggatcgcc
     1681 gcctgggcga tgacgctgat tattatctcc ggtgaaattg atgtcagcgt tgggccgatg
     1741 gtggcttttg tctcggtgtg cctggcattt ttgctgcaat ttgacgttcc gctggcgatt
     1801 gcttgtctgc tggtgttgct gttaggtgcg ctgatgggaa cgctcgccgg ggtgctgcgc
     1861 ggcgtgt
//
LOCUS       DAMZFT010000314         1830 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.317, whole genome shotgun sequence.
ACCESSION   DAMZFT010000314 DAMZFT010000000
VERSION     DAMZFT010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1830)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1830)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1830
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.317"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(<1..459)
                     /locus_tag="PN977_005314"
     CDS             complement(<1..459)
                     /locus_tag="PN977_005314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312656.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3748 domain-containing protein"
                     /protein_id="HDI6045207.1"
                     /translation="MKQITFAPRNHLLTNTNTWTPDSQWLVFDVRPSGASFTGETIER
                     VNIHTGEVEVIYRASQGAYVGVVTVHPKSEKYVFIHGPENPDETWYYDFHHRRGVIVE
                     GGKVSNLDAMDITAPYTPGALRGGSHVHVFSPNGERVSFTYNDHVMHQLDS"
     gene            560..1624
                     /gene="cbrA"
                     /locus_tag="PN977_005315"
     CDS             560..1624
                     /gene="cbrA"
                     /locus_tag="PN977_005315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin M resistance protein"
                     /protein_id="HDI6045208.1"
                     /translation="MEHFDVAIIGLGPAGSVLARTLASKMQVIALDKKHQCGTEGFSK
                     PCGGLLAPDAQRSFIRDGLTLPVDVIANPQIFSVKTVDVAASLTRNYQRSYININRHA
                     FDLWMKSLIPASVEVYHDSLCRKIWREDDKWHVIFRADGWEQHITARYLVGADGANSM
                     VRRHLYPDHQIRKYVAIQQWFAEKHPVPFYSCIFDNAITDCYSWSISKDGYFIFGGAY
                     PMKDGQTRFATLKEKMSAFQFQFGKAVKSEKCTVLFPSRWQDFVCGKDNAFLIGEAAG
                     FISASSLEGISYALDSAEILRSVLLKQPEKLNTAYWRATRKLRLKLYGKIVKSRCLTA
                     PALRKWIMRSGVAHIPQLKD"
     gene            complement(1621..>1830)
                     /locus_tag="PN977_005316"
     CDS             complement(1621..>1830)
                     /locus_tag="PN977_005316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDI6045209.1"
                     /translation="SSLAPMRLIGLTGGVFNFAGGLGGITVPLVVGYLAQGYGFAPAL
                     VYISAVALIGALSYILLVGDVKRVG"
BASE COUNT          450 a          474 c          492 g          414 t
ORIGIN      
        1 cgaatcgagt tgatgcatta catggtcgtt ataggtaaag ctcacccttt caccgttcgg
       61 gctaaagaca tgcacatggc tgccgccgcg cagtgctcct ggggtgtacg gcgcggtaat
      121 atccattgca tcgagattgc tcaccttgcc gccttcaaca atcactccac gccgatgatg
      181 gaagtcgtaa taccatgttt catcaggatt ttccgggccg tggatgaaaa catatttctc
      241 tgacttcgga tgaacggtca ccacgccgac gtacgcgccc tgtgacgcgc gatagataac
      301 ctcgacctcg ccggtatgga tattcacacg ctcaatggtc tcgccggtaa acgacgcgcc
      361 agaaggacgc acgtcaaata ccagccactg gctgtcgggc gtccaggtat tggtattggt
      421 gagcaggtga ttacggggag caaaggttat ctgtttcatg gcgcggtcct gatacaaaac
      481 gggtcccgcc atcatacttc acaaggactt cactttcaga caatagtctt tcgccatcgt
      541 gatgaatgtg agtgaaaaga tggaacattt cgacgtggcg attattggcc tcggcccggc
      601 agggtcggtg ttggcacgaa cgttagccag caaaatgcag gtgatcgcgc tggataaaaa
      661 gcaccagtgt ggtactgaag gtttcagcaa accctgcggc ggtctgctgg caccggacgc
      721 gcagcgatct tttattcgcg atggactgac gcttcctgtc gatgtgatcg ccaatccaca
      781 gattttcagc gtcaaaactg tcgacgtcgc cgcatcgctc acgcgtaact accagcgaag
      841 ctatatcaat attaatcgcc atgctttcga cttgtggatg aaatcgctga tccccgccag
      901 cgttgaggtt taccacgaca gcctgtgccg aaaaatctgg cgtgaggatg ataaatggca
      961 tgtcattttt cgtgcagacg gttgggagca gcatattact gcccgctatc tggtcggtgc
     1021 cgatggcgca aactcgatgg tgcggcgaca tctctacccg gatcatcaaa ttcgtaaata
     1081 tgtcgctatc cagcagtggt tcgcagagaa acatccggtg ccgttctact cctgcatctt
     1141 tgataatgcg ataactgact gttactcatg gagtatcagc aaagacggtt attttatctt
     1201 tggcggtgcc tatccaatga aagacggtca gacgcgtttc gcgacgctga aagagaaaat
     1261 gagcgccttt cagttccagt ttggcaaagc ggtgaaaagt gagaaatgca cggtgctgtt
     1321 tccctcacgc tggcaggatt ttgtctgcgg taaggacaat gcctttctga ttggtgaagc
     1381 ggcgggattt atcagcgcca gctcgctgga ggggattagc tatgcgctgg atagcgcaga
     1441 gattctgcgt tcggtgttac tgaagcagcc agaaaagctc aataccgctt actggcgcgc
     1501 cacccgcaaa ctgcgtttaa agctctatgg caagatagtc aaaagccgat gcctgaccgc
     1561 accggcttta agaaagtgga ttatgcgcag tggtgtggcg catattccac agttgaaaga
     1621 ttagccaacg cgcttcacat cgcccaccag caggatgtaa gagagcgcgc caatcaacgc
     1681 gacggcggag atataaacca gtgcaggcgc gaaaccgtaa ccctgcgcca ggtaccccac
     1741 caccagcgga acggtgatgc cgcccaaacc accggcaaag ttaaacacgc cgccggttaa
     1801 accaatcagg cgcatcggtg ccagagaaga
//
LOCUS       DAMZFT010000315         1775 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.318, whole genome shotgun sequence.
ACCESSION   DAMZFT010000315 DAMZFT010000000
VERSION     DAMZFT010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1775)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1775)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1775
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.318"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..348
                     /locus_tag="PN977_005317"
     CDS             <1..348
                     /locus_tag="PN977_005317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000871564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HDI6045210.1"
                     /translation="LKTIAQQESGLVATAINHNKNKSGKIISTDYGIMQINSVHIPEL
                     KKLGVIKNKDELLKKPCLNIHTGAWILARHFQRCGVNWECLGSYNAGFSKNNTARRMI
                     YARKIHNRYFRQY"
     gene            365..1015
                     /locus_tag="PN977_005318"
     CDS             365..1015
                     /locus_tag="PN977_005318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001532121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HDI6045211.1"
                     /translation="MSALLSMTIIIIILAVILYLAYITAALYLSAVHDTRPPRPVVYV
                     CCLLAIVSVSLNGAYGVEATGLLFLSILTGLLTVMTLTDIAVCRLPRIFTLSLIVLGA
                     AFRYSLEELTYSLLNASLWFGMTYLLRQFFITAKGTEALGLGDVFLIAGIAMWTQPQH
                     TPLLITAAASGAFLFILLFCRHRHQQALPFAPFLCASLYALTLLPDSVFRTSEIFT"
     gene            1012..>1775
                     /gene="pilV"
                     /locus_tag="PN977_005319"
     CDS             1012..>1775
                     /gene="pilV"
                     /locus_tag="PN977_005319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001532120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shufflon system plasmid conjugative transfer
                     pilus tip adhesin PilV"
                     /protein_id="HDI6045212.1"
                     /translation="MKKFRHDDDKGFILLEILAGLIVIGIATPLIYSEIENWLNEQLY
                     QSAASHADAYNTAARNYIADNNATLHNGSLPANFTADDLIRKGYLKQGFNHSPFGQSY
                     ITGIRRNQTTGRLEALTCSTGGQNIKEDGLRSVAGQLPGLGGYIGKNGTATGAFGAWT
                     DKPGDYGLTCSAGHIAVVMTGDDLQESDRLYRFQVPERPELNQMHTAINMGGNDINNA
                     GNLNGQKATVKGDITSEDGWFITRNNKGWMNTTHGGG"
BASE COUNT          479 a          460 c          434 g          402 t
ORIGIN      
        1 ctgaaaacca ttgcacagca ggaaagcggc ctggtcgcca ctgcgataaa tcacaataaa
       61 aacaaatccg gaaaaatcat cagtacggat tacggaataa tgcagattaa ttcagtgcat
      121 attcctgaac tgaaaaaact gggcgtcatt aaaaacaagg atgaactgct taaaaagccc
      181 tgcctgaata ttcataccgg tgcctggata ctggcccgcc attttcaacg ctgcggcgtt
      241 aactgggaat gccttggcag ctataacgcc ggtttcagca aaaataacac agcccggcgc
      301 atgatatatg cccgaaaaat ccacaaccgg tatttcagac aatattaata aatacgaggt
      361 gacggtgagc gcattactga gcatgacaat aataatcatc atcctggcgg ttattcttta
      421 tcttgcatac atcaccgccg ctctctacct gtcagcagta cacgacaccc ggccgcccag
      481 gccggtggtg tacgtgtgtt gccttctggc catagtgagt gtcagcctca acggcgcata
      541 tggtgttgaa gccacggggc tgttattcct ttccattctg acgggattat taactgtaat
      601 gacgctgacg gacatcgccg tctgtcggct tccccggata ttcacgcttt cactgattgt
      661 tctgggggca gcgttcaggt acagcctgga ggagctcaca tacagcctcc tcaacgcatc
      721 actctggttc ggcatgacat acctgctacg gcagtttttc ataacggcaa aaggaacaga
      781 agcactcggc cttggtgatg tattcctgat agcaggaatt gcaatgtgga cacagccaca
      841 acacacgccg ctgctaatca ccgccgcagc ttccggggca tttctgttta tcctcctctt
      901 ctgccgtcac agacatcagc aggcactgcc gttcgccccg tttctctgcg catcactgta
      961 tgcactaacc ctcctccctg attccgtttt ccgcacttcg gagatattca cgtgaaaaag
     1021 ttcagacacg atgacgacaa aggttttatt ctgcttgaaa tactggcggg actgatagtg
     1081 attggtatcg ccacgccgct gatttattcc gaaatagaaa actggctgaa tgaacagctt
     1141 taccagagtg ctgcttctca tgcagatgcc tacaataccg cagccagaaa ttacattgcc
     1201 gataacaatg ccactctgca caacggcagc cttccggcaa actttacagc cgatgatttg
     1261 attcgcaagg gatacctgaa acagggattt aatcattctc cgtttgggca gagttatatc
     1321 acgggtatcc gccgtaacca gactacagga cgtcttgaag ccctgacctg ctcaacaggg
     1381 gggcagaata tcaaagaaga tggtctgcgc agtgtggcag gacagcttcc cgggctgggt
     1441 ggttatattg gtaaaaacgg aacagcaacc ggcgcattcg gcgcctggac agacaaaccg
     1501 ggggattacg gactcacctg ttcggcaggt cacattgccg tcgtcatgac gggtgatgat
     1561 ctgcaggaaa gcgacaggtt gtatcgtttc caggtcccgg agagaccaga gctgaaccag
     1621 atgcacacag ctatcaacat gggcggaaat gacatcaata acgccggtaa cctcaatggt
     1681 cagaaagcca cggtgaaagg cgatataacc agcgaagacg gctggtttat caccagaaac
     1741 aacaaggggt ggatgaacac gacgcacgga ggcgg
//
LOCUS       DAMZFT010000316         1767 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.319, whole genome shotgun sequence.
ACCESSION   DAMZFT010000316 DAMZFT010000000
VERSION     DAMZFT010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1767)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1767)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1767
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.319"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(<1..238)
                     /gene="tnpA"
                     /locus_tag="PN977_005320"
     CDS             complement(<1..238)
                     /gene="tnpA"
                     /locus_tag="PN977_005320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HDI6045213.1"
                     /translation="MGNEKSLAHTRWNCKYHIVFAPKYRRKVFYREKRKATGSILRKL
                     CEWKSVRILEAECCADHIHMLVEIPPKMSVSGFMG"
     gene            complement(469..1375)
                     /locus_tag="PN977_005321"
                     /pseudo
     CDS             complement(469..1375)
                     /locus_tag="PN977_005321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000878026.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
BASE COUNT          410 a          468 c          382 g          507 t
ORIGIN      
        1 atcccataaa gccggatacg ctcattttgg gcgggatctc cacaagcata tggatatgat
       61 ctgcacagca ctcagcttca agaatccgta cacttttcca ctcacacagc tttctcaaaa
      121 tactgcctgt tgctttacgc ttctctctgt agaacacttt tcttcggtat tttggcgcaa
      181 aaactatgtg atatttacag ttccatcggg tatgcgctaa gctcttttcg ttccccattg
      241 ggaccccctt ttgatttctt gtttgacact tgcagttgcc agaccgcaag gtgttttaac
      301 aaatcaaaag gggttttaat aactggctca aagctgaaag ctttccggcc cccagcctag
      361 ctggggtttt tctgtgcaca aaaatccgga aacgagtgag cgtttcagga ttcttaaaca
      421 gaccctggat cggtcaactg ctcctaaact gatcggcatt acacctgtcg gctgccgttt
      481 tttcagctcc gtgatccatg ggccgggttc tttggtaagc aatgctgccg cccttgcccc
      541 atggataaat aatgtccggt ggtaggtatc cccacgtttg cttatcccca gcatgcgaac
      601 cttcccagcg gaccccgtct gcttcggcac cagacagaca taagcgtcaa actcacgtcc
      661 ggatttaaat gctgaggcct cgcccatggt ggcgaccacc gccgtagcga ttaacggccc
      721 cacaccgggg atttccatta ggcgcttaca gttttcgctc tgccgtgcca cgctgttaat
      781 ctgtttatca aggccatcta tctgcatgcc cagctcactc agtctgttgt actggtattc
      841 aagcacagtg atgagatacg gcggcaggtt cgtcttcatt cgctctaggg ctgtcggtag
      901 ctttctgtcc atcgctgtcc aatctttgtg gaggatttca ccaaactcga ggagcgtgcc
      961 aagggtatta atatgcgagg ttctgaactt caccagttgt ctgcgcgaac ggtgcaggac
     1021 cagaactgac tgcttctctt ccgttttaac agcgatatct ttactgggct gctgtaccgc
     1081 catccagata gcacgcgcat ccatcacatc atttttgttg cccatgacga atgctttaac
     1141 gaagcgagct tgcaacagcc gaactttgtg tcccagtttt ttccagttcc ctcgcccaat
     1201 gttgggagcc tccacaagcc tccataccta tcagacaggg ttcccggttg ctgaagaacg
     1261 tcagaaaatc cttgctacgt agctgcttgt ccaccacttc gcccgtgtac tcattgatga
     1321 agtgaacctg aatcagatac tttgcgatat caacgccaac cggtgtatat ttcatttgtg
     1381 gagtcgccag tctgagggcg cttttgcatc ccgtattggg cactatgatg ccggaaatct
     1441 gagaggctcc acagcagaca attttaccct tcatccatca cagtgcacct ctcttaacag
     1501 ggcaggatgt gttcagtaca tttctccttt tatggttaca gtttatccat catggcgtaa
     1561 ttgaaggctg tcgtgaagga gaggaagagc atctcatcac ttacaaacaa atacattaat
     1621 gataaccatt tgcatttgta tagattatgc tataaaacat agccgttgct atgtttgttc
     1681 taattttgct cactataggt actgaattat gcactcaata aaaaaattaa ccatgctctt
     1741 ggggttcctc gccctcacct gctcgat
//
LOCUS       DAMZFT010000317         1729 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.320, whole genome shotgun sequence.
ACCESSION   DAMZFT010000317 DAMZFT010000000
VERSION     DAMZFT010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1729)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1729)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1729
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.320"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            1005..>1729
                     /locus_tag="PN977_005322"
     CDS             1005..>1729
                     /locus_tag="PN977_005322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000486835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDI6045214.1"
                     /translation="MFRKIKIRKMTLNRALDKYLKTVSVHKKGHLQEFYRVNVIKRHP
                     MAERYMDEITTVDIATYRDQRLAQINPRTGRQITGNTVRLELALLSSLFNIASVEWGT
                     CRMNPVELVRKPKISSGRDRRLTSGEERRLSRYFRDKNQQLYVIFHLALETAMRQGEI
                     LTLRWEHLDLQHGVAHLPETKNGLPRDVPLSRKARNYLQILPQQINGNVFSYTSSGFK
                     SAWRTALLDLKIENLHFHDLRHEA"
BASE COUNT          456 a          437 c          391 g          445 t
ORIGIN      
        1 caccaggaag gttgaggcat gtcactgtaa ttgtgccacc ggactcataa tttccccacg
       61 cataattatt cacataccaa accttgtttt ggtcggcgta tatctgctga aaaccaccaa
      121 aaggcgagcc attaaggtat gtccacgagc agtacgcatg tacgccaatg ctcagattac
      181 gagtattttt cccgatattg tatgtactgg tagtgaagtt catttttgtc gttgtccaga
      241 taccggattg gcacggaggc cagtgccaat ccggtgtgtg gagaacttcc ggttcctata
      301 acggtagtta cagtaatctt ggatcacaca gaggatcatt tactggtcgc aatacgggaa
      361 gtggtacgct gtttgtttac gcctctggag ggaatggtgg ttccgccggg ggagattgtg
      421 ctaacacatc cagattgcag ggatacgttg caggggcatt gattagcaca aatgcgagta
      481 ataacccatc ttatgggaag acagcattca tctcttttgc tgtccctgct ggcgcaactt
      541 atcagatcac ctcttatccg gcccaaaact actcttgtgg ctcaggggtg ttctcggttt
      601 atgcatatca gatgtagcac cgcattcaac taaaccgtaa tgaagttgaa acaatgtgcc
      661 agaaatcatc tggcgcataa cgctacagag cgaaaacagc ttgtgatggt ccctgttcca
      721 ccaacaatcc agcgattgtt cgcccagtcg ggataagttg agtccggtga gttgaacggg
      781 ttacggttgt cctgcgacca ggcggttaat tgatatccgc ctgaaaccaa agtactacca
      841 ctcggacaca ctgcatagga cactgtatgt ccacatccac tcgttccgta aacagtgaaa
      901 tgttcctttt ctttgttacc cttccacgaa ccggattggc acggcgatat tgcatttccg
      961 tttcactatt cctaccttcc ccgtttttat cgtggaaggc agatatgttt cgaaaaatca
     1021 aaattcgcaa aatgactctc aaccgggcgc tggacaaata cctgaaaacc gtttccgtcc
     1081 ataaaaaagg tcatctgcag gagttttatc gcgtcaatgt gattaaacgt caccctatgg
     1141 ccgagcgcta tatggatgaa atcaccaccg ttgatatcgc cacttaccgg gatcagcgtt
     1201 tggctcaaat taacccacga acagggcgtc aaatcaccgg aaacaccgtc cgtcttgaac
     1261 ttgctctttt atcatcatta ttcaatatcg ccagtgtgga atgggggaca tgccgtatga
     1321 atcctgttga actggtccga aaacccaaaa tcagtagtgg acgcgatcgt cgcctgactt
     1381 ctggcgaaga acgccgactc tcccgctatt tcagagataa aaatcagcag ctctacgtta
     1441 tcttccatct ggccctggaa acagcaatgc gacaggggga aatcctcaca ttacgctggg
     1501 aacacctcga tttgcagcac ggagtggcgc atcttcctga aacgaaaaat ggtttaccac
     1561 gcgatgtccc actctcccgg aaagctcgca actacctgca aattctgcca caacagataa
     1621 acggcaatgt gttcagttac acctcatccg gtttcaaaag cgcctggcgt accgcacttc
     1681 tggacctgaa aatagaaaat ctgcattttc atgacctccg tcatgaggc
//
LOCUS       DAMZFT010000318         1721 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.321, whole genome shotgun sequence.
ACCESSION   DAMZFT010000318 DAMZFT010000000
VERSION     DAMZFT010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1721)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1721)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1721
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.321"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            258..1673
                     /gene="gltX"
                     /locus_tag="PN977_005323"
     CDS             258..1673
                     /gene="gltX"
                     /locus_tag="PN977_005323"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HDI6045215.1"
                     /translation="MKIKTRFAPSPTGYLHVGGARTALYSWLFARNHGGEFVLRIEDT
                     DLERSTPEAIEAIMDGMNWLSLEWDEGPYYQTKRFDRYNAVIDQMLEEGTAYKCYCSK
                     ERLEALREEQMAKGEKPRYDGRCRHSHEHHADDEPCVVRFANPQEGSVVFDDQIRGPI
                     EFSNQELDDLIIRRTDGSPTYNFCVVVDDWDMEITHVIRGEDHINNTPRQINILKALN
                     APVPVYAHVSMINGDDGKKLSKRHGAVSVMQYRDDGYLPEALLNYLVRLGWSHGDQEI
                     FTREEMIKYFTLNAVSKSASAFNTDKLLWLNHHYINALPPEYVATHLQWHIEQENIDT
                     RNGPQLADLVKLLGERCKTLKEMAQTCRYFYEDFAEFDADAAKKHLRPVARQPLEVVR
                     DKLAAITDWTAENVHHAIQATADELEVGMGKVGMPLRVAVTGAGQSPALDVTVHAIGK
                     TRSIERINKALAFIAERENQQ"
BASE COUNT          442 a          421 c          460 g          398 t
ORIGIN      
        1 cgctgtgtgg agtcgcatta tagggagagt tcaaaatgag tcaacgcatt ttctaaagaa
       61 attgttcgtt cgtcgtaaat ttaagcaaga tgatcgcaaa acaggccgtg ctgcgcaatt
      121 tctcaacgaa aacaataatg catcatgtag caacccgaac tacattgagg aatcaggcgg
      181 gagtgataga atatcgccca ctcaattttt ccaggatttg ccggttgtcg gcatctttct
      241 aaacgtaagg ccatttcatg aaaatcaaaa ctcgcttcgc gccaagccca acaggctatc
      301 tgcacgttgg cggcgcgcgt actgctcttt actcttggct ttttgcacgt aaccacggcg
      361 gtgagttcgt gctgcgtatt gaagacaccg atcttgagcg ttccacgccg gaagctatcg
      421 aagccattat ggatggcatg aactggctga gcctggagtg ggatgaaggt ccgtactacc
      481 agaccaaacg ttttgatcgc tacaatgcgg tgatcgatca gatgctggaa gagggcactg
      541 cttataaatg ctattgctct aaagagcgcc tggaagcgct gcgcgaagag caaatggcga
      601 aaggtgagaa gccgcgttat gacggtcgct gccgccacag ccatgagcat cacgctgatg
      661 atgaaccgtg tgtcgtgcgt tttgctaacc cgcaggaagg ttctgttgtt tttgacgatc
      721 agatccgtgg tccgatcgaa ttcagcaacc aggagctgga tgatctgatt atccgtcgta
      781 ccgatggttc tccaacctat aacttctgtg tagtggttga cgactgggat atggaaatca
      841 cgcacgtcat ccgtggtgaa gaccatatca acaacacgcc gcgccagatc aacatcctga
      901 aagcgctgaa cgcgcccgta ccggtttacg cgcacgtttc tatgatcaac ggtgatgacg
      961 gtaaaaaact gtccaaacgt cacggggcgg tcagcgtaat gcagtatcgt gatgacggtt
     1021 atttgccaga agcactgctg aactatctgg tgcgtctggg ctggtcccac ggcgatcagg
     1081 aaatcttcac tcgtgaagag atgatcaaat acttcactct gaatgccgtc agcaaatctg
     1141 ccagtgcgtt caacaccgac aagctgctgt ggctaaacca tcactacatt aacgcactgc
     1201 cgccggagta tgtggctacc cacttacagt ggcacatcga gcaggaaaat atcgataccc
     1261 gtaacggccc gcaactggct gatctggtaa aactgctggg tgaacgctgc aaaacgctga
     1321 aagagatggc acagacttgc cgttatttct acgaagattt tgctgagttc gatgccgacg
     1381 ccgcgaaaaa acatctgcgt ccggtagcgc gtcagccgct ggaagtggtt cgtgacaaac
     1441 tggccgcgat tactgactgg accgctgaaa acgttcatca cgctattcag gcgacggcgg
     1501 atgagctgga agtgggtatg ggtaaagttg gtatgccgct gcgtgtcgcc gttaccggtg
     1561 cggggcagtc tccggcactg gacgttaccg ttcacgcgat cggtaagacc cgcagtatcg
     1621 agcgtatcaa caaagcgctg gcttttattg cggaacgcga aaaccagcag taattagcgc
     1681 ataaaagata aacggcagga gaaatacccc tgccgttttt t
//
LOCUS       DAMZFT010000319         1719 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.322, whole genome shotgun sequence.
ACCESSION   DAMZFT010000319 DAMZFT010000000
VERSION     DAMZFT010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1719)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1719)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1719
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.322"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..548
                     /locus_tag="PN977_005324"
     CDS             <1..548
                     /locus_tag="PN977_005324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001549957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDI6045216.1"
                     /translation="QEFDLRAFAGRAGYDVVGIFKETGSGTKLDRAERKKVLALAQSR
                     QIDAILVTELSRWGRSTLDLLNTLRELENWKVSVIAMNGMAFDLSSPYGRMLATFLSG
                     IAEFERDLISERVKSGLAVAKARGKRLGRQAGVRPKSDRLLPKVVAMRAEGRSYRWIA
                     RELGISKNTVADIVQRHRANA"
     gene            715..1629
                     /locus_tag="PN977_005325"
     CDS             715..1629
                     /locus_tag="PN977_005325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HDI6045217.1"
                     /translation="MIPDKSVEILLGELSRDIAQRTPGTGDFPTAVEGLELFRRNEPA
                     PPVSCLVPPSIVLVADGAKIMWVGGEPYEYNAEKFLITSLDLPASSEILQASTSQPCV
                     GVAYKLDQRVLMELIAQGSLPPVKKRDAGTSVGIGTITDALLEPFCRLLSLLDEPEAI
                     PVLGPLIQREIHYRLLMSDQSDHLRQIAAVDGHGYRIGKAIDWLKTNIASPLRVEELA
                     SRVQMSTPSFQHHFRQLAGLSPLRYQKWLRLNEARRLMLNEHYDVTTAAYAVGYESLS
                     HFSREYTRMFGESPKRDITELRKSAGKL"
BASE COUNT          404 a          445 c          474 g          396 t
ORIGIN      
        1 gccaggaatt tgatctgcga gccttcgccg gccgtgccgg ctacgacgtg gtgggaatat
       61 ttaaggaaac aggttcagga actaaactcg accgggccga gcgaaagaaa gtcctggcgc
      121 ttgcccagtc cagacaaatt gatgcaatcc tggtcactga gctttcccgg tgggggcgct
      181 cgacgctcga tctgctcaat acgctacgtg aactggagaa ctggaaggtt tccgtgatag
      241 ccatgaatgg aatggcgttc gatctttcgt cgccgtatgg acgaatgctg gcgacgtttc
      301 tttccggcat tgcggagttt gagcgggatc tcatcagcga gcgggtcaag tcaggccttg
      361 ctgttgcgaa ggcacgtggt aagaggcttg gtcgtcaggc cggagtgcga ccaaaatcag
      421 accgactttt gcctaaggtg gttgcgatga gggccgaggg acgcagctat cgctggatcg
      481 cacgcgagct cggtatcagc aagaataccg tcgctgacat cgtgcaacga cacagagcta
      541 acgcttaggg tgtcatttcg ccctcagccg gaaccgaccc catataaggt gttattaaaa
      601 tactctgcat tatttgcctg atcctctgaa cctaatctca aagcgtgtat ttcagttttc
      661 atagctgcta agttgataac ctcaaaagag gtcaaaagtg tggggcatta cagaatgatt
      721 cctgacaaat cggttgaaat actgctgggt gagctatcga gggatattgc acagcgcaca
      781 cccggcaccg gcgatttccc taccgccgta gaagggctcg aattgtttcg ccgaaacgaa
      841 cccgctcccc cggtaagctg cctggtccct cccagtatcg ttctggtggc cgatggcgcg
      901 aagatcatgt gggttggggg tgaaccttat gaatataatg cggaaaaatt cctgattacc
      961 tctcttgacc tgccggccag ttcggaaata ttgcaggctt cgacatccca gccctgcgtc
     1021 ggcgtagcct ataagcttga ccagcgcgtg ctaatggaat taattgccca gggaagcctg
     1081 ccgccggtca aaaaacgaga cgcgggaacg agtgtcggga taggtactat taccgatgct
     1141 ttgctggagc ctttctgccg tctgctttct ttgcttgacg aacctgaagc cattcctgtt
     1201 ctcgggccgc tgatccaaag agagatacac taccggcttt tgatgagcga tcagtccgat
     1261 catctcagac agattgcagc agtcgacggc catggctacc gtatcggtaa agctatcgac
     1321 tggttaaaga ccaatattgc atcaccttta cgcgtcgagg aactggcaag tcgggtgcaa
     1381 atgagcacgc cctctttcca acaccatttc aggcagcttg cggggctgag cccgctacgg
     1441 tatcagaaat ggctgcgcct caatgaagcc aggcgactga tgctgaatga acattacgat
     1501 gtcaccacag cagcctatgc ggtcggttac gaaagcctgt cccatttcag ccgggaatat
     1561 acgcggatgt tcggggaatc gcccaagagg gatattactg aactgagaaa gtcagcgggt
     1621 aagctttaat caattctgga ccgcctgtac catgcctggt cttcgatatc ccccggcacg
     1681 tgagtcgaat acccggaact ttatccccgc cctgaggga
//
LOCUS       DAMZFT010000320         1703 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.323, whole genome shotgun sequence.
ACCESSION   DAMZFT010000320 DAMZFT010000000
VERSION     DAMZFT010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1703)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1703)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1703
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.323"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(<1..707)
                     /locus_tag="PN977_005326"
     CDS             complement(<1..707)
                     /locus_tag="PN977_005326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001262762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
                     /protein_id="HDI6045218.1"
                     /translation="MANENLDIDSKTAEELYEWYLDDKLIINRRYQRKLVWSLEEKTA
                     LISSMTQQYPIPLLLFVSIDNKREILDGMQRLEALMSFIEQRFSLNGKYFNLDAIALT
                     KQLKDSGKLQQKEPVLSRESSTLIARYRFAISEYSSTEDHIDEVFRRINSNGKILSKQ
                     ELRSAGCVSNFSELVRKISTIIRGDTTHSDIMGLNKIHNISICNDGLDYGINIDNHFY
                     IRNHIISRPSIRDSDDE"
     gene            complement(992..1393)
                     /gene="pemK"
                     /locus_tag="PN977_005327"
     CDS             complement(992..1393)
                     /gene="pemK"
                     /locus_tag="PN977_005327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001398199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin
                     endoribonuclease PemK"
                     /protein_id="HDI6045219.1"
                     /translation="MLKYQLKNENGWMHRRLVRRKSDMERGEIWLVSLDPTAGHEQQG
                     TRPVLIVTPAAFNRVTRLPVVVPVTSGGNFARTAGFAVSLDGVGIRTTGVVRCDQPRT
                     IDMKARGGKRLERVPETIMNEVLGRLSTILT"
     gene            complement(1326..1583)
                     /gene="pemI"
                     /locus_tag="PN977_005328"
     CDS             complement(1326..1583)
                     /gene="pemI"
                     /locus_tag="PN977_005328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000557619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin PemI"
                     /protein_id="HDI6045220.1"
                     /translation="MHTTRLKRVGGSVMLTVPPALLNALSLGTDNEVGMVIDNGRLIV
                     EPYRRPQYSLAELLAQCDPNAEISAEEREWLDAPATGQEEI"
BASE COUNT          477 a          389 c          326 g          511 t
ORIGIN      
        1 tcttcatcgt cagagtctct aatgctaggc ctactaatga tatgatttct aatatagaaa
       61 tgattgtcta tatttattcc ataatcgagt ccatcattgc aaattgatat gttatgtatt
      121 ttattcagtc ccattatatc gctatgtgta gtatctcctc ttataattgt cgatattttc
      181 cttactaact ctgaaaaatt actaacgcac cccgcacttc gtagttcttg ttttgataaa
      241 attttaccat tggaatttat gcgtcgaaac acttcgtcaa tgtggtcctc tgttgaagag
      301 tattcagata tagcaaatct atagcgagca attaatgtag aactttcgcg ggatagcact
      361 ggctcttttt gttgaagttt accactgtct tttagttgtt ttgttaatgc aatggcatct
      421 aaattaaagt atttaccatt taatgaaaat cgctgctcaa taaaactcat taaagcttct
      481 agtctttgca ttccatcaag gatttctctt ttgttgtcaa tggaaacaaa caatagcaaa
      541 gggatagggt attgctgagt cattgatgaa atcagtgctg ttttctcttc gagggaccat
      601 actaattttc tttggtatct tcgatttata attaatttat catccagata ccactcgtat
      661 aattcttctg ctgtcttgga gtcaatatct aagttttcat tagccattag atgtatctcg
      721 agtatttagt taagaaactg ataggtcagt atttgcatca atgttcattg aatttgtatc
      781 cgaagtcaat ctgatgtagg aacttcaaaa ctgcgcaaaa cacgcaaata cgcacaagaa
      841 agtagggctg cgcttggaat tcggaaaata aaccacatca aaactacttt ttagccaaat
      901 aacgtttggg aatcaccaga atggtgggac aacagcggtt ttagtgccct aaatcgtacg
      961 ttttcatcca gttgcccctc aaaccccatg ttcaagtcag aatagtggac aggcggccaa
     1021 gaacttcgtt catgatagtc tccggaaccc gttcgagtcg ttttccgccc cgtgctttca
     1081 tatcaattgt ccggggttga tcgcaacgta caacacctgt ggtacgtatg ccaacaccat
     1141 ccaacgacac cgcaaagccg gcagtgcggg caaaattgcc tccgctggtt acgggcacaa
     1201 caacaggcag gcgggtcacg cgattaaagg ccgccggtgt gacaatcagc accggccgcg
     1261 ttccctgctg ctcatgacct gcggtaggat caagcgagac aagccagatt tcccctcttt
     1321 ccatgtcaga tttcctcctg accagtcgcc ggtgcatcca gccattctcg ttcttcagct
     1381 gatatttcag cattcggatc acactgtgcc agtagctcag ccagtgaata ttgcgggcgt
     1441 ctgtacggct caacaatcag ccggccatta tcaatgacca tgccaacttc attatctgtg
     1501 cccagagaca gcgcattcag cagtgccggt gggacggtca gcataactga gccgccaacc
     1561 ctcttcagtc gggtggtatg cattcttcac ctccataaaa gttatattta aatataacat
     1621 ccactaaaaa aacacaccag gctttaacgc acaatgttta ataaaaatat aactttcaac
     1681 caaacagtaa acccagcgtg gtt
//
LOCUS       DAMZFT010000321         1701 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.324, whole genome shotgun sequence.
ACCESSION   DAMZFT010000321 DAMZFT010000000
VERSION     DAMZFT010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1701)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1701)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1701
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.324"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..978
                     /locus_tag="PN977_005329"
     CDS             <1..978
                     /locus_tag="PN977_005329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020326821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDI6045221.1"
                     /translation="AMNQALAIVVAAQGKLPMSRVWGMGTSASSDGQFFPTARHGEAM
                     NMVNAKYGSVPGLKAYTHVSDQFAPFACQSIPATVSEAPYILDGLLMNEVGRHVREQY
                     ADTAGFTDHLFGASSLLGYNLVLRIRDLPSKRLYVFNPDTTPRELRKLVGGKAREDLI
                     VANWPDIFRCAATMTAGKIRPSQLLRKLASYPRQNNLAVALREVGRIERTLFIIEWIL
                     DTDMQRRAQIGLNKGEAHHALKNALRIGRQGEIRDRTTEGQHYRIAGLNLLTAVIIYW
                     NTVHLGHAVTERRNEGLDVPPEFLPHISPLGWAHILLTGEYLWPKEPKA"
     gene            1011..1595
                     /locus_tag="PN977_005330"
                     /pseudo
     CDS             1011..1595
                     /locus_tag="PN977_005330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001697773.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1592..>1701
                     /locus_tag="PN977_005331"
     CDS             1592..>1701
                     /locus_tag="PN977_005331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000175482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ddrA"
                     /protein_id="HDI6045222.1"
                     /translation="MTLSAIELMDLSDKLDALMSKAAIASGMELLDISDE"
BASE COUNT          409 a          437 c          473 g          382 t
ORIGIN      
        1 gccatgaacc aggcattggc aattgtggtg gccgcgcagg gtaaactgcc gatgtcacgc
       61 gtctggggga tgggcacgtc agcatcgagc gatggtcagt ttttcccgac agcgcggcat
      121 ggcgaagcca tgaacatggt caatgccaaa tatggttctg ttcccggcct caaagcgtat
      181 actcacgtaa gcgaccagtt cgcgccattc gcttgtcagt cgatcccggc gaccgtgagc
      241 gaggcaccgt atattctcga tggactactg atgaacgagg tcggtcgcca tgttcgcgaa
      301 cagtatgccg atacagcagg attcaccgac catttgttcg gagccagtag cctgctcggc
      361 tacaatctcg ttctgcgaat cagggatctg ccatcgaagc ggttgtacgt atttaatccc
      421 gatacgaccc ccagggagtt acgcaagttg gtaggtggaa aagcccggga ggatcttatc
      481 gttgcgaact ggcctgatat tttccgttgt gccgcgacga tgaccgctgg caaaatcagg
      541 cccagccaac tcctgcgcaa gctcgcttct tacccacgac aaaacaacct tgcagttgcg
      601 cttcgtgaag ttggtcgtat tgaacggacc cttttcatta ttgagtggat cctggatacg
      661 gacatgcagc ggcgtgctca gatcggtctt aacaagggag aggcccacca tgcgctcaaa
      721 aatgcgctcc gtatcgggag gcagggggaa attcgcgatc gcacgacaga ggggcagcac
      781 taccgaatcg ctgggctcaa tttattgact gcggtgatca tttactggaa taccgtccat
      841 cttggtcatg ccgtcacgga gcggcggaac gaagggttgg atgttccccc tgaatttctt
      901 ccccacatat ccccattggg ctgggcgcac attctactga ctggcgaata tctttggccc
      961 aaggaaccga aagcttaggg tgtcatttcg ccctcagccg gaaccgaccc caatatgcac
     1021 gctacggcat tgctacttac gataccccgc tttctgatca gcaaatgagt gagtacgacc
     1081 tgaagttatt gcctcgcgag gattctttcg acttcctggc gaagacactt actaatggtc
     1141 cgtttggcaa atatgcacaa aaagctctgg agctggccac cagctcacca gacgagttcc
     1201 gcgtaatgct gaaaactcag tttcaaaaaa ctttccccaa tattgcgttt ccggggggcg
     1261 ctggcaccga gaaaatggtg cagagcatga tcaatgcatt gcaggccgaa gtcggtgaga
     1321 ttactcagcc agaacctgcc ccggcacagc ctgatgaaac ggttagcgaa gcagatgcag
     1381 aggctaataa agccattgaa tatctcaata acgtgatgga tatgcaaagc actgacatgg
     1441 cggagatccg taacgcccgg ggtaatgtcc gggaagcgat tgcagccctt caggctgccg
     1501 ggcgttttga ggaaaacgaa gagctggtta atggcgctgc tcgccacctg gctgatctgc
     1561 tggtagcaat ccagaaagcg ggggtagcgg catgacacta tcagctattg agttaatgga
     1621 tctcagcgat aagttggatg ctctgatgtc caaagcggct atcgcgagtg gtatggagtt
     1681 gctggatatc agcgatgaaa t
//
LOCUS       DAMZFT010000322         1699 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.325, whole genome shotgun sequence.
ACCESSION   DAMZFT010000322 DAMZFT010000000
VERSION     DAMZFT010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1699)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1699)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1699
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.325"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(<1..77)
                     /gene="btuE"
                     /locus_tag="PN977_005332"
     CDS             complement(<1..77)
                     /gene="btuE"
                     /locus_tag="PN977_005332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310444.1"
                     /note="similar to glutathione peroxidase; member of the
                     btuCED operon which is required for vitamin B12 transport
                     across the inner membrane; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HDI6045223.1"
                     /translation="MQDSILTTVVKDIDGEVTTLEKYAG"
     gene            complement(140..1120)
                     /gene="btuC"
                     /locus_tag="PN977_005333"
     CDS             complement(140..1120)
                     /gene="btuC"
                     /locus_tag="PN977_005333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter permease BtuC"
                     /protein_id="HDI6045224.1"
                     /translation="MLTLARQQQRQNIRWLLCLSVLMLLALLLSLCAGEQWISPGDWF
                     TPRGELFVWQIRLPRTLAVLLVGAALAISGAVMQALFENPLAEPGLLGVSNGAGVGLI
                     AAVLLGQGQLPNWALGLCAIAGALIITLILLRFARRHLSTSRLLLAGVALGIICSALM
                     TWAIYFSTSVDLRQLMYWMMGGFGGVDWRQSWLMLALIPVLLWICCQSRPMNMLALGE
                     ISARQLGLPLWFWRNVLVAATGWMVGVSVALAGAIGFIGLVIPHILRLCGLTDHRVLL
                     PGCALAGASALLLADIVARLALAAAELPIGVVTATLGAPVFIWLLLKAGR"
     gene            complement(1221..1520)
                     /gene="ihfA"
                     /locus_tag="PN977_005334"
     CDS             complement(1221..1520)
                     /gene="ihfA"
                     /locus_tag="PN977_005334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HDI6045225.1"
                     /translation="MALTKAEMSEYLFDKLGLSKRDAKELVELFFEEIRRALENGEQV
                     KLSGFGNFDLRDKNQRPGRNPKTGEDIPITARRVVTFRPGQKLKSRVENASPKDE"
     gene            complement(1525..>1699)
                     /locus_tag="PN977_005335"
     CDS             complement(1525..>1699)
                     /locus_tag="PN977_005335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6045226.1"
                     /translation="NLFDVYRGKGVAEGYKSLAISLILQDTSRTLEEEEIAATVAKCV
                     EALKERFQASLRD"
BASE COUNT          435 a          487 c          406 g          371 t
ORIGIN      
        1 ttaccggcgt acttctccag cgtggtcact tcaccgtcga tatctttcac tacggtcgtc
       61 agaatggaat cttgcatcgt ttctcctggg tgtggtcagt aaaaatctta gcttttaatc
      121 atagaccgtc tttttgcggc taacgtcctg cttttaacaa taaccagata aacaccggcg
      181 cacccaacgt tgcggtgacc acgccaatag gcagctctgc ggcagctaat gccaggcgcg
      241 ctacaatatc ggccagcagc aatgcgctcg cccctgccag cgcgcagccg ggaagtaata
      301 cgcgatgatc ggttaaacca cacagccgga gaatatgtgg gatcaccaga ccaataaagc
      361 cgatagcacc cgccagcgcc acactgacgc caaccatcca gccggtcgct gccaccaaca
      421 cattgcgcca gaaccacagg ggtaaaccca gttgccgcgc cgagatctcg ccaagtgcta
      481 acatattcat tggcctggac tgacaacaga tccacaacaa cacggggatc aatgccagca
      541 tcagccagct ttgccgccag tctacgccgc caaaaccgcc catcatccag tacatcagct
      601 gacgtaaatc aacagaggtg gaaaagtaga tagcccacgt cattagtgcg ctacagataa
      661 tccctaatgc aacgccagcc agcaataacc gactggtcga aagatgacga cgggcgaaac
      721 gtaagagtat taaagtgatg ataagcgcgc cagcaatcgc acacagccct agcgcccagt
      781 tggggagttg cccttgccca agcaataccg cggcgataag ccccacgcct gcgccgttag
      841 agacgccaag tagtccaggt tctgccagag gattttcgaa caacgcctgc attacagcgc
      901 cggatatagc cagcgccgca ccaaccagca atacagccag cgtacgtggc aggcgaattt
      961 gccagacgaa cagttcgcca cgaggagtaa accagtcacc tggcgagatc cattgttcac
     1021 cggcgcaaag gcttaagaga agcgccagca gcatcaaaac tgacaggcat aataaccagc
     1081 gaatattttg tcgctgttgt tggcgggcaa gtgtcagcat ggtatccgtt ctgctgaagt
     1141 gtcatggcgt tgattttacg gtgactcttc gacagtgaaa agaaaaaagg ccgcagagcg
     1201 gcctttttag ttagatcaga ttactcgtct ttgggcgaag cgttttcgac ccggcttttt
     1261 aacttctgcc cgggtctgaa ggtcaccacg cgccgtgctg taatgggaat atcctcgccc
     1321 gttttcgggt tacgtcccgg gcgttgattc ttatcacgca gatcgaagtt accaaaacca
     1381 gagagtttca cctgttcgcc gttttccaga gcgcgacgga tctcttcgaa aaacagttca
     1441 accagttctt tggcatcccg cttgctaagc ccaagcttat caaacagata ttctgacatt
     1501 tcagcttttg taagcgccat aggttcaatc cctcaatgat gcctggaatc gctcttttaa
     1561 tgcctctaca catttggcga cggtagcggc aatctcctct tcttcgagtg tacggctggt
     1621 atcttgcagg atcaggctta tggcgaggct cttatacccc tccgcaacac ccttaccgcg
     1681 gtacacgtca aataagttt
//
LOCUS       DAMZFT010000323         1606 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.326, whole genome shotgun sequence.
ACCESSION   DAMZFT010000323 DAMZFT010000000
VERSION     DAMZFT010000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1606)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1606)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1606
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.326"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(114..1088)
                     /locus_tag="PN977_005336"
     CDS             complement(114..1088)
                     /locus_tag="PN977_005336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001422120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03756 family integrating conjugative element
                     protein"
                     /protein_id="HDI6045227.1"
                     /translation="MISLKGRLRAGALLLSVASLPLASASLNTASIIASAAAPDCISW
                     RVSGICYWLYCSTFGCKVRTSVKVTHFIPEVVVSTYTAPGGNPWKEMSLVSRTAGGPE
                     NAITDGLSGLSAGGGNPADMKAQGERRSAIRFKYADALGHPATKLIGGMVPGFSCDSP
                     VTPAMPYFLSTLDSIVWRTGLPESLYPEALVPGKREIGSQADKNMWGSVYPRSGFVMQ
                     TDDDRAAAVVAQRVADIITRTGEPHVYREVKGVKRDGYWPPEAVEENTGTRNHKWQRL
                     TPSVSRSCAVFPDGEHQAAENGNAAFALWQPYSCCKKRGQRFLGSTNF"
     gene            complement(1085..1480)
                     /locus_tag="PN977_005337"
     CDS             complement(1085..1480)
                     /locus_tag="PN977_005337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000286193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03757 family integrating conjugative element
                     protein"
                     /protein_id="HDI6045228.1"
                     /translation="MYRIFCSLFFLPLAASAGTTIYTDSQHLPVNTPDGVRVVLLDAP
                     EQLQNRFFGSLPADADKAEALVRARMQSPEWQMMQAELAAHYRDVAHAWSLGVKKYPA
                     VVFDDSEVVYGTTDVVVAGKLRTEGGVQP"
BASE COUNT          367 a          506 c          410 g          323 t
ORIGIN      
        1 gtgctgtcat tcagtttcgg cagggaaaga agatcagcca tcaccggagt actgcagcac
       61 agcatcacac tcagtgccac cacacctgtt ttcatcttca tcatcatggt tccttaaaaa
      121 ttcgtactgc caaggaatcg ctgcccgcgc tttttgcagc aactgtaggg ctgccacagt
      181 gcaaaggcgg cattaccgtt ctccgctgcc tggtgttcac catccgggaa aacggcacag
      241 gaacgcgaca ctgacggcgt cagtcgctgc catttgtgat tgcgggtgcc ggtgttttcc
      301 tccaccgcct ccgggggcca gtagccgtca cgtttcacgc ccttcacctc cctgtagaca
      361 tgcggctccc ccgtccgcgt gataatgtcg gccacccgct gggccaccac cgccgcggcc
      421 cggtcatcat cggtctgcat cacaaaaccg cttcttggat aaacactccc ccacatgttt
      481 ttatcggcct ggctgccaat ctcgcgtttg ccgggaacca gtgcttcggg atacagggat
      541 tcaggtaatc ccgttcgcca gacgatgctg tccagggtac tcaggaaata gggcatggcc
      601 ggcgtcaccg gtgaatcaca gctgaatccg ggcaccatcc cgccgataag cttcgttgcc
      661 ggatgcccca gcgcatctgc gtatttgaag cgaatggctg aacgccgctc cccctgtgct
      721 ttcatatccg ccggatttcc cccgcctgca ctcagccccg acagaccgtc agtgatggca
      781 ttctccgggc cacctgccgt ccggctgacc agcgacatct ctttccaggg attaccaccc
      841 ggtgccgtat aggtggaaac aaccacttcc gggatgaaat gcgtcacctt cacggaggtt
      901 cggaccttgc agccaaacgt gctgcagtaa agccagtaac aaatcccgct gacacgccag
      961 ctgatacagt ccggtgccgc cgcgctggcg ataatgctgg cagtattcag tgacgcagac
     1021 gccagaggca gtgatgccac actcagtaac agcgcgccgg cacgcagacg acctttcagg
     1081 gatatcatgg ctgcactcct ccctcagtac ggagtttccc ggccaccacc acatcagtgg
     1141 tgccgtaaac cacctcactg tcatcaaaca ccacggcagg atatttcttc acgcccagcg
     1201 accaggcatg tgccacatcg cggtaatgag cagccagttc cgcctgcatc atctgccatt
     1261 cgggagactg catacgtgcc ctgaccagcg cttcagcctt atccgcatca gcaggcaacg
     1321 agccaaaaaa gcggttctga agctgctccg gtgcatccag cagaaccacc ctgacaccat
     1381 cgggagtatt aacggggagg tgctgagaat cggtataaat tgttgtgcca gcagacgctg
     1441 ccagcggcag gaaaaacagg gaacagaaaa tccggtacat tgtacgcctc cgtcagacaa
     1501 tatccgactt acagcgtgcg cataagcagc accaatagca gcaataaatt caaaaccgga
     1561 aactgaaaat ttaagagggg tgcgttgcgt gagaattaag cgtccg
//
LOCUS       DAMZFT010000324         1583 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.327, whole genome shotgun sequence.
ACCESSION   DAMZFT010000324 DAMZFT010000000
VERSION     DAMZFT010000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1583)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1583)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1583
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.327"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..176
                     /locus_tag="PN977_005338"
     CDS             <1..176
                     /locus_tag="PN977_005338"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309017.1"
                     /note="catalyzes the formation of 3-phosphonooxypyruvate
                     and glutamate from O-phospho-L-serine and 2-oxoglutarate;
                     catalyzes 4 phosphohydroxy L-threonine from 2-oxo 3
                     hydroxy 4- phosphobutanoate; required both in major
                     phosphorylated pathway of serine biosynthesis and in the
                     biosynthesis of pyridoxine; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoserine transaminase"
                     /protein_id="HDI6045229.1"
                     /translation="DSALDKLFLEESFAAGLHALKGHRVVGGMRASIYNAMPLEGVKA
                     LTDFMVEFERRHG"
     gene            247..1530
                     /gene="aroA"
                     /locus_tag="PN977_005339"
     CDS             247..1530
                     /gene="aroA"
                     /locus_tag="PN977_005339"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HDI6045230.1"
                     /translation="MESLTLQPIARVDGTINLPGSKSVSNRALLLAALAHGKTVLTNL
                     LDSDDVRYMLNALKALGVSYTLSADRTRCEIIGNGGPLHAKSALELFLGNAGTAMRPL
                     AAALCLGSNDIVLTGEPRMKERPIGHLVDALRLGGAKITYLEQENYPPLRLQGGFTGG
                     NVDVDGSVSSQFLTALLMTAPLAPEDTVIRIKGDLVSKPYIDITLNLMKTFGVEIENQ
                     HYQQFVVKGGQSYQSPGTYLVEGDASSASYFLAAAAIKGGTVKVTGIGRNSMQGDIRF
                     ADVLEKMGATICWGDDYISCTRGELNAIDMDMNHIPDAAMTIATAALFAKGNTTLRNI
                     YNWRVKETDRLFAMATELRKVGAEVEEGHDFIRITPPEKLKFAEIATYNDHRMAMCFS
                     LVALSDTAVTILDPKCTAKTFPDYFEQLARISQAA"
BASE COUNT          387 a          374 c          412 g          410 t
ORIGIN      
        1 cggacagtgc gcttgacaaa ttgttccttg aagagtcttt tgctgctggc cttcatgcgc
       61 tgaaaggtca ccgtgtggtc ggcggaatgc gtgcgtctat ttataacgcc atgccactgg
      121 aaggcgttaa agcgctgaca gacttcatgg ttgagttcga acgccgtcac ggctaatgtc
      181 gaaattttgc ttaatcccca cagccagcct gtggggtttt tatttctgtt gtagagagtt
      241 gagttcatgg aatccctgac gttacaaccc atcgcccgtg tcgatggcac tattaatctg
      301 cccggttcca agagcgtttc taaccgcgct ttattgctgg cggcattagc acatggcaaa
      361 acagtattaa ccaatctgct ggatagcgat gatgttcgct atatgcttaa tgcgttaaag
      421 gcattagggg taagctatac gctttcagcc gatcgtacgc gttgcgaaat catcggtaac
      481 ggcggtccat tacacgcaaa aagcgccctg gagttgttcc tcggtaacgc cggaacggca
      541 atgcgtccgc tggcggcagc tctttgtctg ggtagcaatg atattgtgct gaccggtgaa
      601 ccgcgtatga aagaacgccc cattggtcat ctggtggatg cgctgcgcct gggcggggcg
      661 aagatcactt acctggaaca agaaaattat ccaccattgc gtttacaggg cggctttact
      721 ggcggcaacg ttgacgttga tggctccgtt tccagccagt tcctcaccgc actgttaatg
      781 actgcgcctc ttgcaccgga agatacggtg attcgtatta aaggcgatct ggtctctaaa
      841 ccttatatcg acatcacact caatctgatg aagacgtttg gtgttgaaat tgaaaatcag
      901 cactatcaac aatttgtcgt taaaggcggg cagtcttatc agtctccggg tacttatttg
      961 gtcgaaggcg atgcatcttc ggcttcttac ttcctggcag cagcagcaat caaaggcggc
     1021 actgtaaaag tgaccggcat tggacgcaac agtatgcagg gtgatattcg ctttgctgat
     1081 gtgctggaaa aaatgggtgc gaccatttgc tggggcgatg attatatttc ctgcacgcgt
     1141 ggtgaactga acgccattga tatggatatg aaccatattc ccgatgcggc gatgaccatt
     1201 gccaccgcgg cgttatttgc aaaaggcaac accacgctgc gcaatatcta taactggcgt
     1261 gttaaagaga ccgatcgcct gtttgcgatg gcaacagaac tgcgtaaagt cggtgcggaa
     1321 gtcgaagagg ggcacgattt tattcgcatt acgccaccgg aaaaactgaa atttgctgag
     1381 atcgccactt ataatgatca ccggatggcg atgtgtttct cgctggtcgc attatcagat
     1441 acggcagtga cgattcttga tcccaaatgc acggccaaaa catttccgga ttatttcgag
     1501 cagctggcgc ggattagcca ggcagcctga atgaacaacg tgcaataaat agccaaatct
     1561 ttctttatca aaacgtcggc aca
//
LOCUS       DAMZFT010000325         1510 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.328, whole genome shotgun sequence.
ACCESSION   DAMZFT010000325 DAMZFT010000000
VERSION     DAMZFT010000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1510)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1510)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1510
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.328"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(<1..230)
                     /gene="pphC"
                     /locus_tag="PN977_005340"
     CDS             complement(<1..230)
                     /gene="pphC"
                     /locus_tag="PN977_005340"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase PphC"
                     /protein_id="HDI6045231.1"
                     /translation="MSWRLVYASTVGTSHISADLPCQDACQMQVAWLNDQQPLLSVFV
                     ADGAGSVSQGGEGAMLAVNEAMAYMSQKVQGG"
     gene            complement(227..886)
                     /locus_tag="PN977_005341"
     CDS             complement(227..886)
                     /locus_tag="PN977_005341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HDI6045232.1"
                     /translation="MSEQITFATSDFASNPEPRCPCILLLDVSGSMNGRPINELNAGL
                     VTFRDELLADPLALKRVELGIVTFGPVHVEQPFTSAANFFPPILFAQGDTPMGAAITK
                     ALDMVEERKREYRANGISYYRPWIFLITDGAPTDEWQAAANKVFQGEEDKKFAFFSIG
                     VQGADMKTLAQISVRQPLPLQGLQFRELFSWLSSSLRSVSRSTPGTEVVLEAPKGWTS
                     V"
     gene            complement(1108..1167)
                     /gene="ibsA"
                     /locus_tag="PN977_005342"
     CDS             complement(1108..1167)
                     /gene="ibsA"
                     /locus_tag="PN977_005342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsA"
                     /protein_id="HDI6045233.1"
                     /translation="MMKHVIILVILLVISFQAY"
     gene            complement(1440..1496)
                     /locus_tag="PN977_005343"
     CDS             complement(1440..1496)
                     /locus_tag="PN977_005343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HDI6045234.1"
                     /translation="MMKILIIVVLLVISFPAY"
BASE COUNT          388 a          398 c          369 g          355 t
ORIGIN      
        1 tccccgccct gcactttttg cgacatataa gccatcgctt cattgacggc gagcatcgcg
       61 ccttcgccac cctgcgagac actgcctgcg ccatcagcga caaataccga caataatggc
      121 tgctggtcat ttaaccaggc aacctgcatc tggcaggcat cctggcaagg caagtccgcg
      181 ctgatgtgcg acgttccaac ggttgatgca tagaccagac gccagctcac actgacgtcc
      241 agccttttgg cgcttccagc acaacttccg ttcccggcgt ggagcgggaa accgaacgca
      301 gcgaactgga taaccagcta aacagttcac ggaattgtaa tccctgcaat ggcaaaggct
      361 gacgaacgct gatttgtgcc aaagtcttca tatccgcccc ctgaacaccg atggaaaaga
      421 aggcaaactt cttatcttct tccccctgaa acactttgtt ggcggcagcc tgccactcat
      481 cggttggagc gccatcggtg atcaggaaaa tccacgggcg ataatacgaa ataccatttg
      541 cacgatactc acgtttccgc tcctctacca tatccagggc tttggtaatg gcagcgccca
      601 ttggtgtatc gccctgggca aacaggatgg gcgggaaaaa attagcggcg ctggtaaagg
      661 gttgttccac atgcactggg ccgaacgtca caataccgag ttccactctt tttaaggcca
      721 gcgggtcagc aagcagctca tcgcgaaagg taaccaatcc ggcgttaagt tcattaatgg
      781 gtctaccgtt catagaacca gaaacatcca gcagcaaaat gcaggggcaa cgtggttccg
      841 ggttactggc aaaatcgctg gtggcaaacg tgatttgttc gctcataaga aatcctttct
      901 tagagaatta ttgaggtccg gtatcatacc gattgcaata atgaaataag agcaaacgaa
      961 gaaactttca gaattaggcc cggcggcgca gaaaattttg ctattcgcct cgcaagaacg
     1021 gagagccgtc gattgacact aagggcggag tgacataatt tcaggagtga gggttaggga
     1081 gaggtttccc ccctccccct ggtgttctta gtaagcctgg aagctaatca ctaagagtat
     1141 caccagtatg atgacgtgct tcatcataac cctttcctta ttaaaagccc tcttctccgg
     1201 gagagggttt cccgtttcag cgtcccgctg aaatcgtcgg cttacctcct ttcgccctga
     1261 atgcagtcta tcgctaacgc gtgaacaaaa tagtttcctc tgtgattatt ccgtaacgac
     1321 tcgcaaagaa tatttgctgg caggatcgca gactacaaag cctgcggatt gacaatctta
     1381 tcgtgaaggc ataatttcag gagtgagggt agagcggggt ttcccccgcc ctggtagtct
     1441 tagtaagcgg ggaagcttat gactaagagc accacgatga tgagtatctt catcatgacc
     1501 ctttccttat
//
LOCUS       DAMZFT010000326         1506 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.329, whole genome shotgun sequence.
ACCESSION   DAMZFT010000326 DAMZFT010000000
VERSION     DAMZFT010000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1506)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1506)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1506
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.329"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(64..285)
                     /locus_tag="PN977_005344"
     CDS             complement(64..285)
                     /locus_tag="PN977_005344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF987 domain-containing protein"
                     /protein_id="HDI6045235.1"
                     /translation="MKIITRGEAMRIHQQHPTSRLFPFCTGKYRWHGSAEAYTGREVQ
                     DIPGVLAVFAERRKDSFGPYVRLMSVTLN"
     gene            complement(354..830)
                     /locus_tag="PN977_005345"
     CDS             complement(354..830)
                     /locus_tag="PN977_005345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RadC family protein"
                     /protein_id="HDI6045236.1"
                     /translation="MQQISFLPGEMTPGERSLIQRALKTLDRHLHEPGVAFTSTRAAR
                     EWLILNMAGLEREEFRVLYLNNQNQLIAGETLFTGTINRTEVHPREVIKRALHHNAAA
                     VVLAHNHPSGEVTPSKADRLITERLVQALGLVDIRVPDHLIVGGSQVFSFAEHGLL"
     gene            complement(846..1331)
                     /locus_tag="PN977_005346"
     CDS             complement(846..1331)
                     /locus_tag="PN977_005346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HDI6045237.1"
                     /translation="MTTSSHNSTTSSVSVTTASGNNPPQLVATPVPDEQRISFWPQHF
                     GLIPQWVTLEPRVFGWMDRLCEDYCGGIWNLYTLNNGGAFMAPEPDEDDDETWVLFNT
                     MNGNRAEMSPEAAGIAACLMTYSHHACRTECYAMTAHYYRLRDYALQHPEYDAIMRII
                     D"
BASE COUNT          355 a          376 c          437 g          338 t
ORIGIN      
        1 acagaaaatc ggttaagaat ggtccggtag tggcgataat tctgctcatc agaatgccct
       61 gactcagttc agggtgacac tcatcaggcg gacatacggg ccaaaactgt ccttacggcg
      121 ttctgcaaat acagccagca caccgggaat atcctgcact tcacgaccgg tatacgcctc
      181 agcactgccg tgccagcggt atttaccggt acagaacgga aaaagacggg atgtcggatg
      241 ttgttggtga atacgcatgg cttcaccacg ggtgatgatt ttcatggtgt gatacctctg
      301 aagacagaag ataaaagtga aaacaggtgt gatgtggtgg tgacggtgac gggttaaagc
      361 agaccgtgtt ccgcaaagga gaaaacctgg ctgccaccga ctatcagatg gtccggtacc
      421 cggatatcca ccaggcccag tgcctgtacc agacgttccg tgataaggcg gtctgccttg
      481 ctgggtgtga cttcaccgga cgggtgattg tgtgccagca ccacggcagc ggcattgtgg
      541 tgcagggcgc gtttaatcac ctcccgggga tggacttccg tgcggttgat ggtgccggtg
      601 aagagggttt caccggcaat cagctgattc tggttattca gatacagcac ccggaattct
      661 tcacgctcca gtcccgccat gttcagaatc agccattccc gtgccgcacg ggtggaggtg
      721 aaggccacgc cgggttcatg aagatggcgg tccagggttt tcagggcccg ctgaatgaga
      781 ctgcgctcgc cgggcgtcat ttctccgggc agaaaggaaa tctgttgcat tgttcctgcc
      841 ccctttcagt caataatgcg cataatggcg tcgtattccg gatgttgcag ggcgtaatcc
      901 cgcagacggt aataatgagc cgtcattgca taacactccg tacggcaggc gtgatgactg
      961 tacgtcatca gacaggcagc aatgccggcg gcttccgggc tcatttcagc gcggttaccg
     1021 ttcatggtat tgaacagtac ccatgtttcg tcatcatctt catccggttc aggtgccata
     1081 aatgccccgc cgttgttcag ggtatacaga ttccagatac caccgcagta gtcttcgcaa
     1141 agacggtcca tccagccgaa gacacggggc tccagggtca cccactgtgg aatgaggcca
     1201 aaatgctgcg gccagaagct gatacgttgt tcatcaggga cgggagtggc aaccagctga
     1261 ggtgggttat tccctgatgc agtagttacg gaaacagaag atgtggtgga attatgcgaa
     1321 gaggttgtca tgagattatt ccttataaaa agcaaatgaa tggaagaatg cccgggggaa
     1381 gggacagccg tgagtcagaa ctgcgctttc agggaaacgg catcagcgca tactctccag
     1441 cagtgtttct gccatcaccc acaacgcccg gttgagctta atgtcggtgt cgatgctgtg
     1501 aatggc
//
LOCUS       DAMZFT010000327         1498 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.330, whole genome shotgun sequence.
ACCESSION   DAMZFT010000327 DAMZFT010000000
VERSION     DAMZFT010000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1498)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1498)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1498
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.330"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            310..1461
                     /locus_tag="PN977_005347"
     CDS             310..1461
                     /locus_tag="PN977_005347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000514103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6045238.1"
                     /translation="MGKELDDICTSCPYIDTVKRHKQQLGAIEEYTQWLKKEPRASYF
                     FLFQLYTRIHNTFFSQKQQLPFTPGGTHCPEPDVTLRDLTLSPGYHSDYAPQPIPEMD
                     SSAVVPPTNENTSPPEDTPDNTPAGGNTGQAEKTRNAGLTPIPEKRSGMPPEHLRFAT
                     GFPSQPKIAGPREKPMRTVHPDKIYREIIWFCSSYLLKSGPEATRTIINSVFSEWASI
                     NNDYPSPFSWVDSRDSEQCDWLWNAMQIRCVGTPLNPLTPEQKYWFACATFDNWEGWN
                     EQQVQFLLESNPRRNRAKFTQVSFQAPRIQHKAILLDELKSAREQQKRRDERADGSVP
                     LKLSGKIHKQLESIARSRGVLPKKLLNEMIEQAYHDLVATRQNSQIDSR"
BASE COUNT          431 a          373 c          348 g          346 t
ORIGIN      
        1 tttttatata aaaatgagaa gtttacggga tgtatatccg gcacactatg accggagaat
       61 atcagtcaag cttaaaaaga caataaatac agtgagttaa atacacagag aaaacgtagt
      121 atcagagggt acatcttaca acagagactg aatttccggt ttttcataac cggcgcattc
      181 cggaaagtgc aggcggcccg gaggttttct gacgccacta cctggtagat tgttctgtac
      241 atcatgtacg gaggtttttg tttgcttttt taaaatctgc aaatgtgaga aaagcaggtg
      301 aggtgtattg tgggaaagga actggatgat atctgcacca gctgtccgta tatcgatact
      361 gtaaaacggc acaaacaaca gctcggtgcc atagaggaat atacacagtg gttaaaaaaa
      421 gaaccacggg catcttattt ttttctgttc cagctgtata cccgcattca caatacattt
      481 ttctcccaaa agcaacaact cccgttcact cccggtggca ctcactgccc ggaacctgat
      541 gtcacccttc gggacttaac gctttcccct ggctaccaca gtgattatgc ccctcaaccc
      601 attccggaga tggacagttc tgctgttgtt cccccaacta atgaaaacac ctcgcctccg
      661 gaagatactc ccgacaacac tccggccgga ggcaataccg gacaagcgga aaagaccaga
      721 aacgcaggac tcacacccat tcctgaaaaa cgttcaggaa tgcccccgga acatctgcgg
      781 tttgccacgg ggtttccatc gcaaccgaag attgccggtc ccagagaaaa gccgatgcgc
      841 accgtgcatc cggataaaat ctaccgtgaa attatatggt tctgctccag ttacctgctg
      901 aaatccggac cagaggctac gaggacgatt atcaactcag tattctctga atgggccagc
      961 ataaacaatg attacccctc ccccttttcg tgggtggaca gcagggacag tgaacagtgt
     1021 gactggttat ggaacgccat gcagatccgg tgtgtgggaa ccccgctgaa tccccttacc
     1081 ccggagcaga aatactggtt tgcctgcgcc acgtttgata actgggaggg ctggaatgag
     1141 caacaggtac agtttttact ggaaagtaat cccagacgga acagagcgaa gtttacgcag
     1201 gtctccttcc aggcccccag gattcagcat aaagcgattc ttcttgatga gctgaaaagt
     1261 gcccgggagc aacaaaaaag gcgtgatgaa cgtgctgatg gttccgtccc tctgaaactg
     1321 tccggaaaaa tccacaaaca gcttgaaagt attgcccgaa gtcgtggtgt cctcccaaaa
     1381 aaactgctga atgaaatgat tgagcaggcg taccatgatt tggtggcaac caggcagaac
     1441 agccaaatcg attccagata atttcagaca gatattattt ccgtgaatcc cccgccac
//
LOCUS       DAMZFT010000328         1485 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.331, whole genome shotgun sequence.
ACCESSION   DAMZFT010000328 DAMZFT010000000
VERSION     DAMZFT010000328.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1485
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.331"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            91..1119
                     /gene="murB"
                     /locus_tag="PN977_005348"
     CDS             91..1119
                     /gene="murB"
                     /locus_tag="PN977_005348"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HDI6045239.1"
                     /translation="MNHSLKPWNTFGIDHNAQHIVCAEDEQQLLNAWQHATAKGQSVL
                     ILGEGSNVLFLEDYRGTVIINRIKGIEIHDEPDAWYLHVGAGENWHRLVKYTLQEGMP
                     GLENLALIPGCVGSSPIQNIGAYGVELQRVCAYVDCVELATGKQVRLTAKECRFGYRD
                     SIFKHEYQDRFAIVAVGLRLPKEWQPVLTYGDLTRLDPTTVTPQQVFNAVCHMRTTKL
                     PDPKVNGNAGSFFKNPVVSAETAKALLAQFPTAPNYPQADGSVKLAAGWLIDQCQLKG
                     MQMGGAAVHRQQALVLINEDNAKSEDVVQLAHHVRQKVGEKFNVWLEPEVRFIGASGE
                     VSAVETIS"
     gene            1116..>1485
                     /locus_tag="PN977_005349"
     CDS             1116..>1485
                     /locus_tag="PN977_005349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin operon repressor"
                     /protein_id="HDI6045240.1"
                     /translation="MKDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLR
                     DWGVDVFTVPGKGYSLPEPIQLLNAEQILGQLDGGSVTVLPVIDSTNQYLLDRIGELK
                     SGDACVAEYQQAGRGRRGRKW"
BASE COUNT          406 a          329 c          374 g          376 t
ORIGIN      
        1 tttctacaaa cactccctgt cgtcataact acaagccatc cccccacaga tacggtaaac
       61 tagcctcgtt tttgcatcag gaaagcagct atgaaccact ccttaaaacc ctggaacaca
      121 tttggcattg atcataatgc tcagcacatt gtatgtgccg aagacgaaca acaactactc
      181 aatgcctggc agcatgcaac cgcaaaagga caatccgttc ttattctggg tgaaggaagt
      241 aatgtacttt ttctggaaga ctatcgcggt acggtgatca tcaaccggat caaaggtatc
      301 gaaattcatg atgaacctga tgcgtggtat ttacatgtag gagccggaga aaactggcat
      361 cgcctggtaa aatacacttt gcaggaaggt atgcctggtc tggaaaatct ggcattaatt
      421 cctggttgtg tcggctcatc acctatccag aatattggtg cttatggcgt agaattacag
      481 cgagtttgcg cttatgttga ctgtgttgaa ctggcgacag gcaagcaagt gcgcttaact
      541 gccaaagagt gccgttttgg ctatcgcgac agtattttta aacatgaata ccaggaccgc
      601 ttcgccattg tagccgtagg tctgcgtctg ccaaaagagt ggcaacctgt actaacgtat
      661 ggtgacttaa ctcgtctgga tcctacaacc gtaacgccac agcaagtatt taatgcggta
      721 tgtcatatgc gcaccaccaa actccctgat ccaaaagtga atggcaatgc cggtagtttc
      781 ttcaaaaacc ctgttgtatc tgccgaaacg gctaaagcat tactggcaca atttccaaca
      841 gcaccaaatt atccccaggc ggatggttca gtaaaactgg cagcaggttg gcttatcgat
      901 cagtgccagc taaaagggat gcaaatgggt ggggctgcgg tgcaccgtca acaggcgtta
      961 gtcctcatta atgaagacaa tgcaaaaagc gaagatgtgg tgcaactggc acatcatgta
     1021 agacaaaaag tgggtgaaaa atttaatgtc tggcttgagc ctgaagttcg ctttattggt
     1081 gcatcaggtg aagttagcgc agtggagacg atttcatgaa ggataatacc gttccgctga
     1141 aattaatcgc gctgttagcg aatggcgaat ttcactctgg tgagcagttg ggtgaaacgt
     1201 taggaatgag ccgggcggct attaataaac acattcagac actgcgtgac tggggcgttg
     1261 atgtctttac cgttccgggt aaaggataca gcctgcctga gcctatccag ttacttaatg
     1321 ctgaacagat attgggtcag ctggatggcg gtagtgtaac tgtgctgccc gttattgact
     1381 ccacgaatca gtaccttctt gatcgtatcg gagagcttaa atcgggcgat gcctgtgttg
     1441 cagaatacca gcaggctggc cgtggtcgcc ggggtcggaa atggt
//
LOCUS       DAMZFT010000329         1463 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.332, whole genome shotgun sequence.
ACCESSION   DAMZFT010000329 DAMZFT010000000
VERSION     DAMZFT010000329.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1463)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1463)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1463
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.332"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..1168
                     /locus_tag="PN977_005350"
     CDS             <1..1168
                     /locus_tag="PN977_005350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS66-like element ISEc23 family transposase"
                     /protein_id="HDI6045241.1"
                     /translation="YLGEVSAEQLELVSSALKVIRTERVKKACTKCDCIVEAPAPSRP
                     IERGIAGPGLLARVLTGKYCEHLPLYRQSEIFARQGVELSRALLSNWVDACCQLMTPV
                     NDALYRYVMNTRKVHTDDTPVKVLAPGQKKAKTGRIWTYVRDDRNVGSSSPPAVWFAY
                     SPNRQGKHPEQHLRPFRGILQADAFTGYDRLFSAEREGGALTEVACWAHARRKIHDVY
                     ISSKSATAEEALKRISELYAIEDEIRGLPESERLAVRQQRSKVLLTSLHEWMVEKNGT
                     LSKKSRLGEAFSYVLNQWDALCYYSDDGLAEADNNAAERALRAVCLGKKNFMFFGSDH
                     GGERGALLYGLIGTCRLNGIDPEAYLRHILSVLPEWPSNRVDELLPWNVVLTNK"
     gene            1326..1457
                     /locus_tag="PN977_005351"
                     /pseudo
     CDS             1326..1457
                     /locus_tag="PN977_005351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000355317.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
BASE COUNT          371 a          358 c          432 g          302 t
ORIGIN      
        1 ttatctgggg gaagtcagcg ctgaacagct ggaactggtg agcagtgccc tgaaagtgat
       61 ccgcacagaa cgggtaaaaa aagcctgtac aaaatgtgac tgtattgttg aagcaccggc
      121 gccgtcccgc ccgatagagc gtggtatcgc gggccccgga ttacttgccc gcgtgttaac
      181 gggaaaatac tgcgaacatc tgccactgta tcgtcagagt gaaatctttg cccgccaggg
      241 tgtcgaactg agccgggcct tactctccaa ctgggttgac gcgtgctgcc agttaatgac
      301 accggtgaat gatgccctgt accgttatgt aatgaacacc cgcaaggttc acactgatga
      361 cacaccggta aaggtactgg caccgggtca gaaaaaggcg aaaacagggc gtatctggac
      421 gtatgtccgg gatgatcgca atgtgggttc gtcatctcct ccagcggtct ggttcgcgta
      481 ctcgccgaac cggcagggga aacacccgga gcaacacctc cgccccttcc ggggtatcct
      541 gcaggcggat gcgttcacag gttacgacag gttgttcagt gcagaacgtg aaggtggtgc
      601 actgacagaa gttgcgtgct gggcccatgc ccggcgaaaa atccacgatg tatacatcag
      661 cagcaaaagt gcgacggcag aagaagccct gaagcgaatc agtgaactgt acgccatcga
      721 ggatgaaata cggggattac cggagtcaga gcgtcttgcc gtcaggcagc agcgaagcaa
      781 agtgttactg acgtcgctgc atgaatggat ggtggagaag aatggtacgc tgtcgaaaaa
      841 atccagactg ggcgaagcgt tcagctatgt actgaatcag tgggatgccc tctgttatta
      901 cagtgatgac ggtctggcgg aggcggataa taatgctgcg gaaagagcgc ttcgtgcagt
      961 ctgtctcgga aagaaaaact ttatgttctt tggcagcgat cacggcggcg agcgtggagc
     1021 actgttgtac gggctgatcg gcacctgccg tctgaacggt atcgatccgg aagcgtatct
     1081 gcgccatatc ctgagcgtac tgccggaatg gccttccaac cgagttgacg aactcctgcc
     1141 atggaacgta gtactcacca ataaataagc gtcaatacgg tgctccgttg acgcttacag
     1201 ccatcaagac ctctactgag aagaaaattc agcagtagag actatgcaga aaagttaatt
     1261 ccaggagtga ctgcaggatg cgaatcaatg atcggacaat gcgaatcagc gatcgagtac
     1321 gatgcgaatc ggcgatcgaa cacgatgcga ttttgcaaca tgcctggctg acggacgtac
     1381 tgacgcgcct gccggagtgg cctgaggacc gactggctga gttgctgcct cttgagggat
     1441 ttaccttatc cgggtgacac aag
//
LOCUS       DAMZFT010000330         1456 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.333, whole genome shotgun sequence.
ACCESSION   DAMZFT010000330 DAMZFT010000000
VERSION     DAMZFT010000330.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1456)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1456)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1456
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.333"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            315..611
                     /locus_tag="PN977_005352"
                     /pseudo
     CDS             315..611
                     /locus_tag="PN977_005352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000952428.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
BASE COUNT          438 a          358 c          358 g          302 t
ORIGIN      
        1 ctctgtggaa acaggggcgc gcattagctt ctcaaccaga gagtgtcaca aagacaaata
       61 ttgctgaaaa tcgcattcat cgttcaggcg tgaatgcccg gagacaaaag ccgctgtcca
      121 cacagtcccc ctctgacctg aacaaaaagc atacacacaa caggctcacc tgaccccgaa
      181 aaaccataac ttcagcaata ccagtgacac cagcacgtta cctcagtgcc acatcttcag
      241 accgcgatca atctgaaatg tgactatcgg ggcaatatcc ctgctttgtg gtacacgata
      301 accagaaaac atcccctgaa ccgccagcca gaaagactgg ccacaaaagg ggtaagctga
      361 aatcgtttat ggattacatc gacatatgcc tggcagagaa tgtccggaat gacgaggtta
      421 tctttgcgga gattaaggca atggtatacg ggccgacgtt ccatgccgcg ttactacatt
      481 cagcccggac gtaaaatgcg cccgtcaaaa agaacggttc gcttcgaaac tcagccagga
      541 taccacctct ccgccacacc cgggaagaag ttgatatggt ggttgccgga caacagcgct
      601 cctggattga ctgctgcatc actcaaccac gctgaatatc aaaggagagc gttaccggtt
      661 aaaagagaaa cgtaaagctg tggtgatgac ccaaaacgca acgcctgtca gtgaggatga
      721 aatggtgaaa agcggacagc atcagtaacg gaagtatctt agcgggtacg aaaatggcag
      781 gatcgtgaca ttgacagaat atctcctgat catcctgaat gactgcagtt gctgtagaaa
      841 agtaagctgt ctccacccac ggcaccactt aacaggcatt ccgtaacttc ccccagactt
      901 actatgacgc attatcggtg cactgaaaac gggagagtgc gcttaaaacc gcagaccgca
      961 tgcgggtgtt actgcatgac agtctggccg tattgtgaga tgtcggcagg aaccgatgtc
     1021 cgcgcagggg ggaacttcct gcagtacctg acgcttcaaa tggtattaac cgtagggtgt
     1081 tgttggttcc ggatcgcgga aatgggagca caggtctgct acggggtgta cctctgacag
     1141 taaatccggc tcgctcatct gacagcctct ctcccggaaa agaccaaaaa tgagatcgtc
     1201 tcctcaacag cagagaagag gtcttgacag acaggctaca ttgcatacga tttatgaaga
     1261 aaaagacagc aaatatggag gttatagcgg agaataccct acgaaattcc tatgaaaaac
     1321 gattgaaaaa aatatcaaat tcgattcgtt tttatatgct ttttgggagc gtggcgatga
     1381 cgaactgttg catgtgcaga ggagaatgca cccggaagca ccagaatcat ccagaaaggc
     1441 agaacgatta cgcatc
//
LOCUS       DAMZFT010000331         1411 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.334, whole genome shotgun sequence.
ACCESSION   DAMZFT010000331 DAMZFT010000000
VERSION     DAMZFT010000331.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1411)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1411)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1411
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.334"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..716
                     /locus_tag="PN977_005353"
     CDS             <1..716
                     /locus_tag="PN977_005353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001569361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDI6045242.1"
                     /translation="PSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLME
                     NSDRCQDIRNLAFLGIAYNTLLRIAEISRIRVKDISRTDGGRMLIHIGRTKTLVSTAG
                     VEKALSLGVTKLVERWISVSGVADDPNNYLFCRVRKNGVAAPSSTSQLSTRALEGIFE
                     ATHRLIYGAKDDSGQRYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNY
                     IRNLDSETGAMVRLLEDGD"
     gene            767..1078
                     /locus_tag="PN977_005354"
     CDS             767..1078
                     /locus_tag="PN977_005354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001749391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysogeny establishment protein"
                     /protein_id="HDI6045243.1"
                     /translation="MRKDFNIDGKYVVLSVSTNIQSPAVIVTVKLSDRMPDIDSISVA
                     FPVKSMRSAEHFVMNATEEEARRGFAKVMSEFGELLGKVNNVLSISSARSKALTASMM
                     K"
     gene            complement(1320..>1411)
                     /locus_tag="PN977_005355"
     CDS             complement(1320..>1411)
                     /locus_tag="PN977_005355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001260625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage antirepressor Ant"
                     /protein_id="HDI6045244.1"
                     /translation="LTPQLTGKGQQWLTRKLLDAGLLKPVAAE"
BASE COUNT          393 a          303 c          353 g          362 t
ORIGIN      
        1 gaccaagtga cagcaatgct gtttcactgg ttatgcggcg gatccgaaaa gaaaacgttg
       61 atgccggtga acgtgcaaaa caggctctag cgttcgaacg cactgatttc gaccaggttc
      121 gttcactcat ggaaaatagc gatcgctgcc aggatatacg taatctggca tttctgggga
      181 ttgcttataa caccctgtta cgtatagccg aaatttccag gatcagggtt aaagatatct
      241 cacgtactga cggtgggagg atgttaatcc atattggcag aacgaaaacg ctggttagca
      301 ccgctggtgt agagaaggca cttagcctgg gggtaactaa actggttgag cgatggattt
      361 ctgtctctgg tgtagctgat gatccgaata actacctgtt ttgccgcgtc agaaaaaatg
      421 gtgttgccgc gccatcatcc accagccagc tatcaactcg cgccctggaa gggatttttg
      481 aagcaactca tcgattgatt tacggcgcta aggatgactc tggtcagaga tacctggcct
      541 ggtctggaca cagtgcccgt gtcggagccg cgcgagatat ggcccgcgct ggagtttcaa
      601 taccggagat catgcaagct ggtggctgga ccaatgtaaa tattgtcatg aactatatcc
      661 gtaacctgga tagtgaaaca ggggcaatgg tgcgcctgct ggaagatggc gattagccat
      721 taacgcgtaa atgattgctc taattatttg atatttatgg tgacatatga gaaaggattt
      781 caacatcgac ggaaaatatg tagtgctgtc tgtaagcact aatattcagt cgccagccgt
      841 cattgtcact gtaaagctga gcgatagaat gcctgatatt gactcaatat ccgttgcgtt
      901 tcctgtcaaa agtatgcgta gtgctgaaca tttcgtgatg aatgccaccg aggaagaagc
      961 acggcgcggt tttgctaaag tgatgtctga gtttggcgaa ctcttgggta aggttaacaa
     1021 tgtcctttca atcagttcag caaggtccaa agcgttaaca gcttccatga tgaaataaaa
     1081 aaaagcctgg caaggagcca ggctgcacaa aagagcgggt ttgtattccg catccaatca
     1141 atcaagaagg agtatagcac acaggtactg aagtgaaaaa atgtgattcg cgataaacaa
     1201 aatatctatc attgctctaa ttgattgcta taattaagcc gcagtttttg tcaactacga
     1261 agacattgcc attatagact ccgtgacatc attggcggcc gaaaggccgc cttttttaat
     1321 cattcagccg ccacaggttt taacaagcca gcatcgagca gtttacgcgt caaccactgc
     1381 tggcctttac ccgttaattg aggcgtcaac c
//
LOCUS       DAMZFT010000332         1339 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.335, whole genome shotgun sequence.
ACCESSION   DAMZFT010000332 DAMZFT010000000
VERSION     DAMZFT010000332.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1339)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1339)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1339
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.335"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            124..624
                     /locus_tag="PN977_005356"
     CDS             124..624
                     /locus_tag="PN977_005356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001368197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HDI6045245.1"
                     /translation="MSICLNGAYGVDATGLLFLSILTGLLTVMTLTDIAVCRLPRIFT
                     LSLIILGAAFRYSQDSLAHALLNASLWFGMIYLLRRIFLAAKGTEALGLGDVFLIAGI
                     AMWTQPQHTPLIITAAASGAFLFILIFCRRRRQLALPFAPFLCASLYVLTLFPDSVIH
                     TSEIFT"
     gene            621..>1339
                     /gene="pilV"
                     /locus_tag="PN977_005357"
     CDS             621..>1339
                     /gene="pilV"
                     /locus_tag="PN977_005357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000714521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shufflon system plasmid conjugative transfer
                     pilus tip adhesin PilV"
                     /protein_id="HDI6045246.1"
                     /translation="MKKFRHDDDKGFILLEILAGLIVIGIATPMIYSEIEDWLNEQLY
                     QSAALHADEYNNAIKNYVADKTSSLSSKTITVNELIQQGYLNNGFSRSPFGHSYITGI
                     RKNNLSGRLEALTCTTGGQDIKEDGLRRIAGQINGLGGFMLQNNSVTGAFGGWTDLGS
                     NYQITCNKGHIAMRMAGKDLEESDRLYRYSVQGRPDLNRMHTGIDMNNNSITNINEAS
                     GKNARFSGDVTSNRWLHPNGGG"
BASE COUNT          345 a          323 c          309 g          362 t
ORIGIN      
        1 atcctgattg tccttgcggc aattctgtat ctttcataca gcgccgccac agtatattta
       61 tcagaagtac acaatacccg cccacacaac gctgcggtgt gcatctgttg tcttctggct
      121 gtcctgagca tttgcctcaa cggtgcatat ggtgttgacg cgacaggact gttattcctt
      181 tccattctga cgggattatt aactgtaatg acgctgacgg atattgctgt ctgtcggctt
      241 ccccggatat tcacgctttc tcttattatt ctgggcgcag cgttcagata cagccaggac
      301 tcactggctc atgccctgct caacgcgtca ctgtggttcg gcatgatata cctgttacga
      361 cgcatttttc tggcagcgaa agggacggaa gcgctcggcc ttggtgatgt gttcctgatt
      421 gcggggatcg caatgtggac gcaaccgcaa cacactccac tcataatcac cgccgcagcc
      481 tccggagcat ttctgtttat cctcattttc tgccgtcgca gacgccagct ggcgctgccg
      541 tttgccccgt ttctctgcgc gtccctgtat gtgctgaccc tcttccctga ttccgttatc
      601 cacacatcgg agatattcac gtgaaaaaat tcagacacga tgacgataaa ggttttattc
      661 tgcttgaaat tctggcaggc ctgatagtaa ttggtatcgc cacgccaatg atttattcag
      721 aaattgaaga ctggctgaat gaacaacttt atcaaagtgc ggctttacat gctgatgaat
      781 ataataatgc aataaagaat tatgtggctg ataaaaccag ctcactttcc agtaaaacca
      841 tcacggtaaa tgaattaatt cagcagggat atctgaacaa cggtttttcc cgctccccct
      901 ttggacacag ctatattacc ggtatcagaa aaaataattt atccggtcgt ctggaagcac
      961 ttacctgcac aaccggagga caggatatta aagaagatgg gttacgtcgc atagcgggcc
     1021 agattaatgg actcggtggt tttatgctcc agaataattc cgttacaggt gcctttggcg
     1081 gctggaccga tctgggaagt aattatcaga ttacctgcaa caaggggcat atcgccatgc
     1141 gaatggctgg aaaagatctg gaggaaagcg atcggcttta ccgctacagc gttcagggaa
     1201 gacccgattt aaacagaatg catactggca ttgatatgaa taataacagc atcaccaata
     1261 tcaatgaagc cagtggtaaa aacgccagat tttcaggcga tgtcacatca aatcgttggt
     1321 tacatccaaa tggtggcgg
//
LOCUS       DAMZFT010000333         1320 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.336, whole genome shotgun sequence.
ACCESSION   DAMZFT010000333 DAMZFT010000000
VERSION     DAMZFT010000333.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1320)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1320)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1320
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.336"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            159..980
                     /locus_tag="PN977_005358"
     CDS             159..980
                     /locus_tag="PN977_005358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000804089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraE"
                     /protein_id="HDI6045247.1"
                     /translation="MKPNSTGIAARMMLSIDRARISEALLNNRQLQSTPLQIRYPQGV
                     REALGIMSEQLSLSVSDLTRILVEDALTEMFLPAENIVHRLICRTEHIMQAHDVCVTT
                     MTTLLAPWNIRPAVFRDPDKLADYLTGEILTALADWFYLNPEWLNGRVRYPLSRAGEW
                     PATQDAFCQLISASDNVDIIIWHSFPFAGVHSQEYCGVLFRKKKEINKAIIYPVLSLS
                     PVRMNKEKENWFHTARNLYAKTSVRTVTLRPAQAEYLMTGKILPAELFSIPLLPW"
     gene            1070..>1320
                     /locus_tag="PN977_005359"
     CDS             1070..>1320
                     /locus_tag="PN977_005359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003557916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraF"
                     /protein_id="HDI6045248.1"
                     /translation="MMMNMHITRTLIASAVLFSFNSTAATSYFEARNDAMGGTGVASS
                     HYGVAPLANPALLTKAGPEDDFSLLIPSVGGQLSDPGNI"
BASE COUNT          362 a          338 c          281 g          339 t
ORIGIN      
        1 gcatgctaaa tcacgtcctg tccgaatcct tttcattcac gcttaaaaca gagttaaaag
       61 cactcttgaa acacactcga tcataacgat cgaaataact cgtttccgtt cagtaaacac
      121 gctctttaga cttaacatat gaaccgcacg gaggtgaaat gaaacccaac agcacaggca
      181 ttgctgcccg catgatgctc tccattgaca gggcccggat tagtgaagct ctgctcaata
      241 accgccagtt gcagtccact cccctgcaga tccgttatcc gcagggcgtc agagaagcac
      301 tcggaataat gagcgagcaa ctctcccttt ctgtatccga cctgacgcgt atcctggtcg
      361 aagatgcatt aactgaaatg tttctcccgg ctgaaaacat cgttcaccgt cttatctgcc
      421 ggacagaaca catcatgcag gcacatgatg tctgcgtaac aacgatgaca acgttactgg
      481 ctccctggaa tatccgtcct gccgttttcc gtgacccgga caaactcgca gattatctga
      541 caggagagat tctgactgct ctggctgact ggttttatct caacccggaa tggctgaacg
      601 gcagggtacg ttatccttta agccgtgccg gtgagtggcc agcaacacag gatgcgttct
      661 gccagcttat ttcagccagc gataatgtgg atattattat ctggcacagt tttccttttg
      721 ccggtgttca ttcacaggaa tattgcggtg tactgttccg caagaaaaaa gaaattaata
      781 aagccatcat ttatcctgta ctttctttat cacctgtaag aatgaataag gaaaaagaaa
      841 actggtttca cactgcccgc aatttatatg caaagacatc cgtccggacc gtaacgcttc
      901 gtccggcaca ggcagaatat ctgatgacag gaaaaatatt acctgctgag ttattcagta
      961 taccgttatt accctggtaa taacgcagag aaaaaaccag taaattttta atggcacagt
     1021 cgtgtcatac cgttgctgta cccaaaacac ctcaaaccag gaaactgaaa tgatgatgaa
     1081 tatgcacatt acccgaactc tcatcgcatc tgctgtttta ttttccttta attcaacagc
     1141 cgcaacttca tattttgaag cccgcaatga tgcaatgggg ggaaccgggg tagcctcctc
     1201 tcactacggt gtggcacctc tggcaaaccc ggccctgctg acaaaagccg gtccggaaga
     1261 tgacttcagt cttctgattc cgtccgttgg tgggcaattg tccgatccgg gtaatatcag
//
LOCUS       DAMZFT010000334         1296 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.337, whole genome shotgun sequence.
ACCESSION   DAMZFT010000334 DAMZFT010000000
VERSION     DAMZFT010000334.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1296)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1296)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1296
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.337"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            2..388
                     /locus_tag="PN977_005360"
     CDS             2..388
                     /locus_tag="PN977_005360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001331609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6045249.1"
                     /translation="MMLAPKNKNAKVKKQKTTQQVSQSERNSLFVVLLIIVVGWCLVF
                     ASNKDIAFISTIIGLIDHFIGPITIAGIVLLIIVAKFTGNLKLGSGEKSNRKTGNRNA
                     HSRSSSKNSFRGGGGSSGGGGSSDRW"
     gene            394..1266
                     /locus_tag="PN977_005361"
     CDS             394..1266
                     /locus_tag="PN977_005361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgcG family protein"
                     /protein_id="HDI6045250.1"
                     /translation="MRIILFLLTLWCLPVLAQQIAVPELRQQVTDITGTLSTSEQQSL
                     TQQLQDITQKTRAQVAVLIVPSTGDDSIEQYATRVFDNWRLGDAKRNDGILIIVAWSD
                     RTVRIQVGYGLEEKVTDALAGNIIRSIMIPAFKKQKLAKGLELAIIALNNQLTSQHQY
                     SSNPSESESASSSDHYYFAIFWVFAVMFFPFWFFHQGNNFCRAFKSSVCISTVYLIDL
                     FLFSDQTFSIAVFSFFFAFTIIMVFTWLCVPQKKASGRSYHSDSGGSGGGSSGGGFNG
                     GGGSSGGGGASGRW"
BASE COUNT          344 a          279 c          289 g          384 t
ORIGIN      
        1 tatgatgctg gccccaaaaa ataaaaacgc taaagtgaaa aaacaaaaga cgactcagca
       61 ggtttctcaa tccgaacgaa actcattgtt tgtcgtgctg ctgataattg ttgtgggatg
      121 gtgcctggta ttcgccagta ataaagatat agcattcatc tcaacaatta ttgggttaat
      181 tgatcatttt atcggcccaa taaccattgc tggcatcgtt ctgctgatca ttgtggccaa
      241 atttactggc aatcttaagc tggggtcagg agaaaaaagc aacaggaaaa cggggaatcg
      301 taatgctcat tctcgctctt cgtcgaaaaa ttctttccgc ggtggcggcg gttcttcagg
      361 aggcggcggt tcatccgacc gttggtgatc gttatgcgta taatcctttt tctactcact
      421 ttatggtgcc ttcctgtgct tgcccagcaa attgctgtgc cggagctgcg ccaacaggtg
      481 accgatatta ctggtacctt aagcacgtct gaacaacaat cactaacaca acagctgcag
      541 gatattacac aaaaaacacg ggcacaggtc gctgtattaa tagtgccatc caccggagac
      601 gattctattg aacaatatgc tacaagagtt tttgacaatt ggcgtttagg agatgccaaa
      661 cgtaatgatg ggatactgat cattgttgcc tggtcggatc gtactgtccg catccaggta
      721 ggttatgggc tggaagaaaa ggtaaccgat gctctggctg gcaatatcat ccgtagcatc
      781 atgatacccg cctttaaaaa acaaaaatta gctaagggat tagaattagc tataatcgct
      841 ttgaataacc aactcacttc acaacaccaa tattcgtcta atccttcaga aagtgaatca
      901 gcgtcttcca gtgatcatta ctactttgct attttttggg tatttgcagt gatgttcttc
      961 cctttctggt tttttcatca aggcaataat ttttgtcggg catttaaaag cagtgtttgt
     1021 atttcaacag tctatcttat agatttattc ctgttctccg atcaaacttt ttccattgct
     1081 gtattttcct tcttttttgc tttcaccatc attatggtct ttacctggtt atgtgtccca
     1141 cagaaaaaag catcaggtag aagttatcat tcagacagcg gcggttctgg tggcggctca
     1201 agtggcggcg gatttaacgg aggtggcggt tcttctggcg gtggcggcgc gtctggccgc
     1261 tggtaactca gccataaaaa atgccagccc gaaggc
//
LOCUS       DAMZFT010000335         1273 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.338, whole genome shotgun sequence.
ACCESSION   DAMZFT010000335 DAMZFT010000000
VERSION     DAMZFT010000335.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1273)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1273)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1273
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.338"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
BASE COUNT          339 a          338 c          293 g          303 t
ORIGIN      
        1 gaaccactat tccctccact ggcgtaaaca aacagcgtcc cacttcctgt attgcggccc
       61 gaaaatgatc ctctgtgtga tccaagatta ctgtaactac cgttagagga accggaagtt
      121 ctccacacac cggattggca cggaggccag tgccaatccg gtgtgtggca aaacagtgac
      181 aaaaaagagc gtttcacagt atccggcggg aatgggtgct ttcagacatc gtcttacgcg
      241 gtatgcccat cggggagtaa gttggtctcc ggcggctaca ggttatccca gtgggtccat
      301 aacggataca actcaccaga tggtagttac cctgattaca aaaacaatcg gtggattgct
      361 actggctctg gctccctgag ttgtttccag gctatagcaa tctgtgagaa ataagccaac
      421 gggagaaaat taaataccgg ccctcggaat ctaaccaaaa gggcaacagt aagcatcgac
      481 gtgctcttca tcaacaccat ctccgatgcc gtacattctt gtacctgtca tgacgtaccc
      541 ggcagggcac tgaatcggct tgtaataaat agaacctcca cttctgccac cgataaaatg
      601 tgcggaggtc gcttcatacc atttacaggc agaatagttc actttattgc ctccaaccca
      661 cacaccggat tggcactggc ctccgtgcca atccggttcg tggaaatcaa taggttcatc
      721 tgcgccgtgg acatcagtaa catccttcac cttgtaccca accacaacga aggctctagg
      781 aaaattcaaa ctttgcgtta atacatatcg catagatgga aaggaaaccg caatgacgcg
      841 tgtctggcct acagacaatc cagatgcaaa cggcgagatg aactggagtg cgtataacgg
      901 tacgttatat ggcagctact acatcactgt tcactgcttc cgctaattca taccagaaca
      961 ctcactgaca caatgcataa gtagtgactg aataatactg attctggttg gtgttttggc
     1021 tgcaaccagc tacccatgca ctgttaccgg agggggaaga acgcttgaga atggcatttg
     1081 aaaagctgca cattccacca cctcctgtaa ctttctttcc tgaagggcaa gtcgcggtgg
     1141 caattggctg attccagtca cttgctgtcg cagtaaccat ctgtactgta ccgccgccat
     1201 ttttctgcca cgtaccggat tggcacgaaa gtatcgcccc tgtactgtcg cggcctacaa
     1261 gtccgtccgg tga
//
LOCUS       DAMZFT010000336         1260 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.339, whole genome shotgun sequence.
ACCESSION   DAMZFT010000336 DAMZFT010000000
VERSION     DAMZFT010000336.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1260)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1260)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1260
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.339"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..681
                     /gene="ptsP"
                     /locus_tag="PN977_005362"
     CDS             <1..681
                     /gene="ptsP"
                     /locus_tag="PN977_005362"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004870786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HDI6045251.1"
                     /translation="EENPFLGWRAIRIAMDRKEILRDQLRAILRASAFGKLRIMFPMI
                     ISVEEVRALRKEIEIYKQELRDEGKAFDESIEIGVMVETPAAATIARHLAKEVDFFSI
                     GTNDLTQYTLAVDRGNDMISHLYQPMSPSVLNLIKQVIDASHAEGKWTGMCGELAGDE
                     RATLLLLGMGLDEFSMSAISIPRIKKIIRNTNFEDAKVLAEQALAQPTTDELMTLVNK
                     FIEEKTIC"
     gene            722..1231
                     /gene="crr"
                     /locus_tag="PN977_005363"
     CDS             722..1231
                     /gene="crr"
                     /locus_tag="PN977_005363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015833547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDI6045252.1"
                     /translation="MGLFDKLKSLVSDDKKDTGTIEIIAPLSGEIVNIEDVPDVVFAE
                     KIVGDGIAIKPTGNKMVAPVDGTIGKIFETNHAFSIESDSGVELFVHFGIDTVELKGE
                     GFKRIAEEGQRVKVGDTVIEFDLPLLEEKAKSTLTPVVISNMDEIKELIKLSGSVTVG
                     ETPVIRIKK"
BASE COUNT          339 a          288 c          321 g          312 t
ORIGIN      
        1 gaagagaacc cgttcctcgg ctggcgcgct atccgtatcg cgatggatcg taaagagatc
       61 ctgcgcgatc agctccgcgc tatcctgcgt gcctcggctt tcggtaaatt gcgcattatg
      121 ttcccgatga tcatctctgt tgaagaagtg cgtgcactgc gcaaagagat cgaaatctac
      181 aaacaggaac tgcgcgacga aggtaaagcg tttgacgagt caattgaaat cggcgtaatg
      241 gtggaaacac cggctgccgc aacaattgca cgtcatttag ccaaagaagt tgatttcttt
      301 agtatcggca ccaatgattt aacgcagtac actctggcag ttgaccgtgg taatgatatg
      361 atttcacacc tttaccagcc aatgtcaccg tccgtgctga acttgatcaa gcaagttatt
      421 gatgcttctc atgctgaagg caaatggact ggcatgtgtg gtgagcttgc tggcgatgaa
      481 cgtgctacac ttctgttgct ggggatgggt ctggacgaat tctctatgag cgccatttct
      541 atcccgcgca ttaagaagat tatccgtaac acgaacttcg aagatgcgaa ggtgttagca
      601 gagcaggctc ttgctcaacc gacaacggac gagttaatga cgctggttaa caagttcatt
      661 gaagaaaaaa caatctgcta atccacgaga tgcggcccaa tttactgctt aggagaagat
      721 catgggtttg ttcgataaac tgaaatctct ggtttccgac gacaagaagg ataccggaac
      781 tattgagatc attgctccgc tctctggcga gatcgtcaat atcgaagacg tgccggatgt
      841 cgtttttgcg gaaaaaatcg ttggtgatgg cattgctatc aaaccaacgg gtaacaaaat
      901 ggtcgcgcct gtagacggca ccattggtaa aatctttgaa accaaccacg cgttctctat
      961 cgaatctgat agcggcgttg aactgttcgt ccacttcggt atcgacaccg ttgaactgaa
     1021 aggcgaaggc ttcaagcgta ttgctgaaga aggtcagcgc gtgaaagttg gcgataccgt
     1081 gattgaattc gatctgccgc tgctggaaga gaaagccaag tctaccctga ctccggttgt
     1141 tatctccaac atggacgaaa tcaaagaact gatcaaactg tccggtagcg tgactgtggg
     1201 tgaaaccccg gttatccgca tcaagaagta attcttgccg cagtgaaaaa tggcgcccat
//
LOCUS       DAMZFT010000337         1240 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.340, whole genome shotgun sequence.
ACCESSION   DAMZFT010000337 DAMZFT010000000
VERSION     DAMZFT010000337.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1240)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1240)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1240
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.340"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            347..>1240
                     /gene="argG"
                     /locus_tag="PN977_005364"
     CDS             347..>1240
                     /gene="argG"
                     /locus_tag="PN977_005364"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HDI6045253.1"
                     /translation="MTTILKHLPVGQRIGIAFSGGLDTSAALLWMRQKGAVPYAYTAN
                     LGQPDEEDYDAIPRRAMEYGAENARLIDCRKQLVAEGIAAIQCGAFHNTTGGLTYFNT
                     TPLGRAVTGTMLVAAMKEDGVNIWGDGSTYKGNDIERFYRYGLLTNAELQIYKPWLDT
                     DFIDELGGRHEMSEFMIACGFDYKMSVEKAYSTDSNMLGATHEAKDLEYLNSSVKIVN
                     PIMGVKFWDESVKIPAEEVTVRFEQGHPVALNGKTFSDDVEMMLEANRIGGRHGLGMS
                     DQIENRIIEAKSRGIYEAPGMA"
BASE COUNT          336 a          289 c          307 g          308 t
ORIGIN      
        1 ctgaaacgtg gcaaattcta ctcgttttgg gtaaaaaatg caaatactgc tgggatttgg
       61 tgtaccgaga cgggacgtaa aatctgcagg cattatagtg atccatgcca cattttgtca
      121 acgtttattg ctaatcacgt gaatgaatat ccagttcact ttcatttgtt gaatactttt
      181 gccttctcct gctctccctt aagcgcatta ttttacaaaa aacacactaa actcttcctg
      241 tctccgataa aagatgatta aatgaaaact catttatttt gcataaaaat tcagtgagag
      301 cggaaatcca ggctcatcat cagttaatta agcagggtgt tattttatga cgacgattct
      361 caagcatctc ccggtaggtc aacgtattgg tatcgctttt tccggcggtc tggacaccag
      421 tgccgcactg ctgtggatgc gacaaaaggg agcggttcct tatgcatata ctgcaaacct
      481 gggccagcca gacgaagagg attatgatgc gatccctcgt cgtgccatgg aatacggcgc
      541 ggagaacgca cgtctgatcg actgccgcaa acaactggtg gccgaaggta ttgccgctat
      601 tcagtgtggc gcatttcata acaccaccgg cggcctgacc tatttcaaca cgacgccgct
      661 gggccgcgcc gtgaccggca ccatgctggt tgctgctatg aaagaagatg gcgtgaatat
      721 ctggggtgac ggcagcacct ataaaggaaa cgatatcgaa cgtttctacc gttacggtct
      781 gctgaccaat gctgaactgc agatttacaa accgtggctt gatactgact ttattgatga
      841 actgggcggc cgtcatgaga tgtctgaatt tatgattgcc tgcggtttcg actacaaaat
      901 gtctgtcgaa aaagcctact ccacggactc caacatgctt ggtgcaacgc atgaagcgaa
      961 ggatctggaa tacctcaact ccagcgtcaa aatcgtcaat ccaattatgg gcgtgaagtt
     1021 ttgggatgag agcgtgaaaa tcccggcaga agaagtcaca gtacgctttg agcaaggtca
     1081 tccggtggcg ctgaacggta aaacctttag cgacgacgta gaaatgatgc tggaagctaa
     1141 ccgcatcggc ggtcgtcacg gcctgggcat gagcgaccag attgaaaacc gtatcatcga
     1201 agcaaaaagc cgcggcattt atgaagcccc tgggatggca
//
LOCUS       DAMZFT010000338         1225 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.341, whole genome shotgun sequence.
ACCESSION   DAMZFT010000338 DAMZFT010000000
VERSION     DAMZFT010000338.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1225)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1225)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1225
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.341"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..264
                     /gene="ychH"
                     /locus_tag="PN977_005365"
     CDS             <1..264
                     /gene="ychH"
                     /locus_tag="PN977_005365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress-induced protein YchH"
                     /protein_id="HDI6045254.1"
                     /translation="ASLLGNVLMGLGLVVMVVGVGYSILNQLPQFNMPQYFAHGAVLS
                     IFVGAILWLAGARVGGHEQVCDRYWWVRHYDKRCRRSDNRRHS"
     gene            complement(319..>1225)
                     /locus_tag="PN977_005366"
     CDS             complement(319..>1225)
                     /locus_tag="PN977_005366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HDI6045255.1"
                     /translation="LPWDLPDSEFTLTWDSIRTLLPAAFSMAMLGAIESLLCAVVLDG
                     MTGTKHKANSELVGQGLGNIIAPFFGGITATAAIARSAANVRAGATSPISAVIHSILV
                     ILALLVLAPLLSWLPLSAMAALLLMVAWNMSEAHKVVDLLRHAPKDDIIVMLLCMSLT
                     VLFDMVIAISVGIVLASLLFMRRIARMTRLAPVVVDVPDDVLVLRVIGPLFFAAAEGL
                     FTDLESRLEGKRIVILKWDAVPVLDAGGLDAFQRFVKRLPEGCELRVCNVEFQPLRTM
                     ARAGIQPIPGRLAFFPNRRAAMADL"
BASE COUNT          304 a          337 c          333 g          251 t
ORIGIN      
        1 gcttcgttac tcggtaacgt gctcatgggg ttgggtctgg tggtaatggt ggtcggtgtg
       61 gggtattcaa tcctcaacca gttaccacag tttaatatgc cccagtattt cgcacatggt
      121 gcagtgctaa gtattttcgt cggtgccatt ctctggctgg cgggtgcccg tgttggcggg
      181 catgaacagg tgtgcgaccg ttactggtgg gttcgccact atgacaaacg ttgccgccgt
      241 agcgataatc gccgtcatag ctaacaaata ctgcagttta ctgaccagtc agtttgctct
      301 gactggtcaa tctcgtactt ataaatccgc catcgccgcg cgacgattcg ggaagaaagc
      361 aaggcggccc gggatcggtt gaatgcctgc gcgagccatc gtgcgcagtg gctggaattc
      421 cacgttgcac acgcgcagtt cacatccttc gggtagacgc ttcacaaaac gctggaacgc
      481 atcaagacca ccagcatcaa gtaccggaac ggcatcccac ttcagaatca caatccgttt
      541 gccttcaaga cgtgactcca ggtctgtgaa taagccttca gcagcagcaa aaaacagcgg
      601 gccaataacg cgcagtacca ggacatcgtc tggaacatct acgactaccg gtgccaggcg
      661 agtcatacgt gcgatacgac gcataaacag cagcgatgcc agcacgatcc ccacgctgat
      721 ggcaataacc atatcaaaca gcacggtcag cgacatgcac aacagcatga caatgatgtc
      781 atctttcggt gcatgacgca gcaagtcgac cactttgtgc gcttcactca tgttccacgc
      841 caccatcaac agcagggctg ccatggcgga aagcggcagc caggagagca gcggtgccag
      901 taccagcagg gcaagaataa ccagaataga gtggatcacc gccgagatag gggaagttgc
      961 cccggcacgg acgttagcgg cagaacgcgc gatggcagct gtagcggtaa tgccaccaaa
     1021 gaacggagcg ataatattcc ccagtccctg tccaaccagt tcgctgttcg ctttgtgttt
     1081 cgttccggtc ataccatcca gtaccacggc gcagagcaga gattcgattg cgccgagcat
     1141 tgccattgag aatgccgcag gcagcagtgt gcgaatagaa tcccaggtta gcgtgaattc
     1201 tgaatcaggc agatcccacg gcagc
//
LOCUS       DAMZFT010000339         1223 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.342, whole genome shotgun sequence.
ACCESSION   DAMZFT010000339 DAMZFT010000000
VERSION     DAMZFT010000339.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1223)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1223)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1223
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.342"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..101
                     /locus_tag="PN977_005367"
     CDS             <1..101
                     /locus_tag="PN977_005367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="protein phosphatase CheZ"
                     /protein_id="HDI6045256.1"
                     /translation="QSLLNGPQVDTSKAGVVASQDQVDDLLDSLGF"
     gene            complement(189..941)
                     /locus_tag="PN977_005368"
     CDS             complement(189..941)
                     /locus_tag="PN977_005368"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6045257.1"
                     /translation="MNVVSNTQQLEQRIADFFTLSDEHKKARVLLDTLACSCPAWIFG
                     GMVRDLGLFGVEGFSSDLDIVIGRSREELFRTLAELPVKQLRFNKFGGIRFRYHDFEF
                     DIWNLNETWAFREKLIFCEDESSLLNTTLMSWDAVLYDLRSEKIISKDNYLTDLNRRH
                     LELVLHTNPNPSGSVTKILRTIFTKQVKSLGPELCHFLHHELTKKRYEVWHHYDVLHY
                     QSSSFSRSQFNALINLLAQQSRFADLKLSNYK"
     gene            complement(910..1119)
                     /locus_tag="PN977_005369"
                     /pseudo
     CDS             complement(910..1119)
                     /locus_tag="PN977_005369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001198675.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyguanosinetriphosphate triphosphohydrolase"
BASE COUNT          346 a          253 c          289 g          335 t
ORIGIN      
        1 accagagttt gcttaatgga cctcaggtcg ataccagcaa agccggtgtg gtagccagtc
       61 aggatcaggt ggacgatttg ttggatagtc ttggattttg atttgtattg ccttaacttc
      121 ggcctgcgcg atgatgccag gccgatagca ggggggcggg tatcgttcag gaagtatgtg
      181 gaggatggtt atttgtaatt tgataatttt agatcagcga agcgtgattg ttgcgccagt
      241 aaatttatca acgcattaaa ttgtgaacga gaaaatgacg atgactggta gtgcagtaca
      301 tcgtaatgat gccaaacttc ataacgtttt ttcgtcagtt catggtgcaa gaaatgacag
      361 agttccggac ctaaactctt tacctgctta gtaaaaatag tgcgtaaaat tttcgttacc
      421 gaaccgcttg gattggggtt agtatgaaga acaagttcaa gatgtctgcg atttaagtcg
      481 gtcaaataat tatctttact gataattttt tcactacgta gatcatagag cacggcatcc
      541 caactcatca gcgtagtgtt taacagactt gattcatcct cacaaaatat aagtttttcc
      601 cgaaatgccc atgtctcatt gaggttccag atatcaaatt cgaagtcgtg ataacgaaaa
      661 cggatcccac cgaacttatt aaaacgcagt tgtttgacgg gcagttcagc caatgtccgg
      721 aagagttcct cacgggagcg accgatgacg atatcaaggt ctgaagagaa tccctcgacg
      781 ccaaacaagc ctaagtcacg caccatcccg ccaaaaatcc aggccggaca ggaacaggcc
      841 agagtatcaa gtaatactct ggctttctta tgctcatcac taagcgtaaa aaaatcggcg
      901 atacgctgtt caagctgttg ggtgtttgag accacattca tacagtgatt ttagctcatc
      961 gtgaaggcgc attaaataac tatccgtcat gcctgagatt gcgtcggtaa gcaactgcgc
     1021 ttctttataa agagcaggga gtgtattggc gggatcttca aacacacggc gataattttc
     1081 agagattgtc ccgtaagcat agcgaccaaa gggagtatcc gacaggtatt cttcacgaga
     1141 aacaaaaagg ttatctgcag acgcggactc gcgttctccg tctcgttgtt tctttgtttt
     1201 gtgtccatgg atgccaatcc aga
//
LOCUS       DAMZFT010000340         1223 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.343, whole genome shotgun sequence.
ACCESSION   DAMZFT010000340 DAMZFT010000000
VERSION     DAMZFT010000340.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1223)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1223)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1223
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.343"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..261
                     /locus_tag="PN977_005370"
     CDS             <1..261
                     /locus_tag="PN977_005370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001289879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6045258.1"
                     /translation="RKAVQAFCDVVGDNTEVIAEVVGRDGVLVILEAMKATGNMPATD
                     AFLAEVRAQGVELLREHPAIQICSLTHVCDEFAAQLRQGEAV"
     gene            258..859
                     /locus_tag="PN977_005371"
                     /pseudo
     CDS             258..859
                     /locus_tag="PN977_005371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001513019.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            861..1082
                     /locus_tag="PN977_005372"
     CDS             861..1082
                     /locus_tag="PN977_005372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000013838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6045259.1"
                     /translation="MANSLLETCNNWQIQRAEILSRNPDMAMTIDNLDTLIERTVRSA
                     IDIAHRVDWDFREAERLAKEQAKAAGKGE"
     gene            1084..>1223
                     /locus_tag="PN977_005373"
     CDS             1084..>1223
                     /locus_tag="PN977_005373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016234701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6045260.1"
                     /translation="MDKNTTAYWSLSLDTECPKCGHNFDLLCDADFWEFSGAKQACEE
                     IKG"
BASE COUNT          336 a          272 c          332 g          283 t
ORIGIN      
        1 cgtaaggcag ttcaggcatt ctgcgatgtt gttggcgaca acaccgaggt tatcgctgag
       61 gtggttgggc gagatggcgt tctggttatt ttggaggcca tgaaggcaac aggaaatatg
      121 ccagccaccg atgcttttct ggctgaagta cgggcgcagg gtgttgaatt gttgcgcgag
      181 catcctgcta tccagatttg ctctttaacg cacgtatgcg acgagttcgc cgcccagctt
      241 cgtcaggggg aagcggtatg agcatagttg atgattcaca tcttacagac gaagtggtta
      301 acgccgcttt cgaaggtacc aatttcgggc gcactgattt ccgcaccatt ctggcagaaa
      361 cagttatgaa gcgtgctgct ggctatcact ctggctacac ggcaacgact atctgtattc
      421 gcctgggatt gcttggcaaa cacgatcgcc cgacaaaact cggcctgacc ttcgctttcc
      481 atcactacta caggccatgc gttcgcgaag cgctgatttc gcataacagc cttgaagtat
      541 gggcaaaact tggagcacag gagtccgcca aatgagcaac atcaacaaac aggcgctgat
      601 tgcaaaaatc aaaaagcagg ccgaaagctt cgacacagtg gtgttgagag aggatgaagc
      661 taacgcgctg ctggatgagc tggaagcagc agagaagcgg attgctgaac tggaagcgcg
      721 ggaaatactg ctcccggaac gtagcagcat gcttcatcga acagattttc acgatgatta
      781 ccaaacggta atggcataca aagtttctga agtcatcgat gcaatccgcg ccgctggcat
      841 tcgtatcaaa ggagagtgat atggctaact cattacttga aacctgcaac aactggcaga
      901 ttcagagggc ggagatttta tctcgcaatc cagatatggc aatgacaatt gataatctgg
      961 acacgctaat tgaacggacc gtgcgttctg caattgatat agcacatcga gtggattggg
     1021 atttcagaga agcggagcga cttgctaaag agcaggcgaa agcagcgggt aaaggagagt
     1081 gatatggata aaaacaccac tgcttactgg agtctttcac ttgataccga atgtcccaaa
     1141 tgcggccaca atttcgatct gctttgcgat gctgatttct gggaattttc tggagcaaaa
     1201 caggcatgtg aagaaataaa agg
//
LOCUS       DAMZFT010000341         1220 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.344, whole genome shotgun sequence.
ACCESSION   DAMZFT010000341 DAMZFT010000000
VERSION     DAMZFT010000341.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1220)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1220)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1220
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.344"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(126..>1220)
                     /locus_tag="PN977_005374"
     CDS             complement(126..>1220)
                     /locus_tag="PN977_005374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kef family K(+) transporter"
                     /protein_id="HDI6045261.1"
                     /translation="KVIAFIAIMMLVGRRLVPWIMARSAATGSRELFTLSVLALALGI
                     AFGAVELFDVSFALGAFFAGMVLNESELSHRAAHDTLPLRDAFAVLFFVSVGMLFDPL
                     ILIQQPLAVLATLAIILFGKSLAAFFLVRLFGHSQRTALTIAASLAQIGEFAFILAGL
                     GMALDLLPQAGQNLVLAGAILSIMLNPVLFALLEKYLAKTETLEEQTLEEAIEEEKQI
                     PVDICNHALLVGYGRVGSLLGEKLLASDIPLVVIETSRTRVDELRERGVRAVLGNAAN
                     EEIMQLAHLECAKWLILTIPNGYEAGEIVASARAKNPDIEIIARAHYDDEVAYITERG
                     ANQVVMGEREIARTMLELLETPPAGKVVTG"
BASE COUNT          298 a          391 c          283 g          248 t
ORIGIN      
        1 tcgtcggttc gtaggccaga taaggcgttc acgccgcatc tggcattttg ctctcaatgc
       61 ctgatgcgac gctggcgcgt cttatcaggc ctacatatcc gcgcctatta tccggcaacc
      121 accgtttacc ccgtcaccac tttacccgcc ggtggtgttt ccagcagttc cagcatagta
      181 cgagcaattt cacgctcacc catcactacc tgattcgcac cacgttccgt gatatacgcc
      241 acttcatcgt cataatgggc gcgggcgata atctcaatat ccggattttt cgcgcgggca
      301 gatgccacaa tctcgcccgc ttcataaccg ttgggaatcg tcaggatcag ccattttgca
      361 cattccagat gcgccagttg cataatttct tcgttcgccg cattgcccaa tactgcgcgg
      421 accccgcgct ctcgcagttc atcaacacgg gttcgtgacg tctcaatcac caccagcgga
      481 atatcagagg cgagcaattt ctcccccagc aggctgccta cacgaccgta acctaccagt
      541 agcgcatggt tgcaaatatc cactgggatc tgcttctctt cttcgatagc ctcttccagc
      601 gtctgctctt ccagcgtttc ggtcttcgcc agatatttct ccagcagcgc aaacaggacc
      661 gggttgagca taatcgacaa aatcgcccct gccagtacca ggttctgccc ggcttgcggc
      721 aacagatcca gcgccatgcc cagtcccgcc aggataaacg cgaactcacc aatttgcgcc
      781 aggctggcgg cgatagttaa tgccgtacgt tgggagtgac caaacagtcg caccaggaaa
      841 aacgcggcta acgacttacc aaacagaata atcgccagcg tcgccagcac tgccagcggt
      901 tgctgaatca gaattaacgg atcaaacaac atcccgacgg agacaaaaaa cagcaccgca
      961 aacgcgtcgc gcaatggcag cgtatcgtgg gcggcacggt gactcagttc agactcgttc
     1021 agtaccatcc cggcaaagaa cgcaccgagt gcaaaggaga catcaaacag ctccaccgca
     1081 ccaaaggcaa tccctaacgc cagcgccagc accgacaggg taaacagctc acgggaaccg
     1141 gttgctgcgc tacgtgccat aatccacggc accagacggc gacctaccag catcataatg
     1201 gcgataaatg cgatcacctt
//
LOCUS       DAMZFT010000342         1216 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.345, whole genome shotgun sequence.
ACCESSION   DAMZFT010000342 DAMZFT010000000
VERSION     DAMZFT010000342.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1216)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1216)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1216
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.345"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            292..982
                     /locus_tag="PN977_005375"
                     /pseudo
     CDS             292..982
                     /locus_tag="PN977_005375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611483.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          357 a          277 c          286 g          296 t
ORIGIN      
        1 cgagacagat ggcacaaagg gctttttccg cggcattatg tctgcctccg cctgaccgtc
       61 aacactgtaa taacagagtg cgccccactg attcagtaca cagctgaacc attctcccag
      121 tctggatttt tggacagcgt ggcatttttc tccaccatcc acgcatgcag tgagtccagt
      181 aacagtttgc tcttcctctg cctgaccaca agatgttctg gttctggcat actgaatttg
      241 gccacctgag cataggtaat atgctcacct tagaacaaca cagatgctcc aatgaaaaaa
      301 ggaaatttca gcgcagagtt taaacgcgaa tccactcaac tagttgttaa ccagaacttt
      361 acggtggcag gtaccatcca aagctatgga tgtcggtctt tccacaatga caagataagt
      421 caaacaactg cgtgatgagc atcagagcaa aacaccaaaa gcctctctga tgacaccaga
      481 acaaatcgaa atacgtgagc tgaggaaaaa gcggcaacgt attgaaatag agaatgaaat
      541 attagaaaag gctacggcgc tcttgatgtc agactcccta aacagttctc gataatcgag
      601 aagctcagag cgcattatct ggtgatcgca ctttgccatg tgtgcaggat tcatcgaagc
      661 agctacagag attggaaaaa ccgtcctgaa aaaaagacgg tagacgggct gtattacgta
      721 gtcaggtaat tgagcttaat ggcggagcaa ggaacatcgc cacaattgca acccggagag
      781 actaccagat gggacgcaag cttgctggca gtctcatgaa agaacagggg ctggtcagct
      841 gtcagccgtt gaccctccgg tacaggcatg gcagtcatga acttgtagct atcctaagaa
      901 cttgaagggc gcttagctgt gacacagcta aatcaagtat ggtgacgtga cctatatctg
      961 gactggtaaa cgatggggat acccgcttac gcgagagcaa taagtggctg gccaccattc
     1021 tcgatacaaa actctgctga atagcagcga ttttcccagc gtggtgcgtc ttttgcattt
     1081 tactggttct gagttatcaa ttatctgttc actaatgact actctaaaaa gttgtatcaa
     1141 tttaagtgat atagggagtc tggagattca gggggccagc ctaggaacat tggttacatt
     1201 tttagtttcc aaacat
//
LOCUS       DAMZFT010000343         1182 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.346, whole genome shotgun sequence.
ACCESSION   DAMZFT010000343 DAMZFT010000000
VERSION     DAMZFT010000343.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1182)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1182)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1182
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.346"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..499
                     /locus_tag="PN977_005376"
     CDS             <1..499
                     /locus_tag="PN977_005376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000926344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="morphogenetic protein"
                     /protein_id="HDI6045262.1"
                     /translation="YQWRIVPLDGWAMEMLEMRRAALPPEDDAEFKEAIVDLRFWEFA
                     YQCELYNDVSGTREEQAERRYETIKRMAIDTEFIKLAAHIRLAHEKLEHGLPCYIDKG
                     EMRLRLPPHKCPNQDKKESTEGAYTRLWVPFRATDFIPQVGIEKLSDLSVQPGFVWGY
                     TDSGR"
     gene            581..>1182
                     /locus_tag="PN977_005377"
     CDS             581..>1182
                     /locus_tag="PN977_005377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000440161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HDI6045263.1"
                     /translation="MERKNANIDDVIRTVETASAKELEELAGIREAVEDLKGGRVATV
                     DPVSHSVSALNRTIENSRPDFVANAPSVAPIVEAMKRLNLGDVSRVVQEDVALQEPQA
                     KSTTRKGKKRRKKAITEDVKAQRTEAAEHAREMFGQKGGAQKSQNQRDARGRFIGKPG
                     SKAAAEDARAERAEKARRKEDDERLNAESGLLKKLSKVAEG"
BASE COUNT          347 a          248 c          331 g          256 t
ORIGIN      
        1 ttatcaatgg cgtatcgtgc cgcttgatgg ttgggcaatg gaaatgctgg agatgcgccg
       61 tgcagcattg ccacctgaag acgacgcgga attcaaagaa gcgatcgttg atttgcgttt
      121 ttgggaattc gcttatcagt gtgaacttta taacgatgtt agcggtactc gtgaagagca
      181 ggctgagcgt cgttatgaaa cgatcaaacg gatggccatt gatactgaat ttataaagct
      241 ggcggcacac atccgactgg ctcatgaaaa gctcgaacat ggtttaccgt gctacatcga
      301 taaaggcgaa atgcgtcttc gtctcccgcc gcacaaatgc ccaaaccagg ataaaaagga
      361 gtccacagag ggtgcgtata cccgtctgtg ggtgcccttt cgggctaccg acttcattcc
      421 acaggtgggg attgaaaagc tatcagacct tagtgtccaa cctggttttg tatgggggta
      481 taccgattca ggacgctgaa aggctcactg aatcctatgc gtttttcctg ttggagaaac
      541 tggaagaaaa acttaaaccg aagcggtagg cgataagatc atggaaagaa aaaacgccaa
      601 cattgacgat gtgataagga cggttgaaac cgccagcgca aaagagctgg aagagcttgc
      661 aggtatccgg gaagctgttg aagatttgaa agggggacgc gttgcaactg ttgatcctgt
      721 ctctcacagt gtgtcggcat taaatcgcac aatcgaaaat tccaggccag actttgtggc
      781 caatgcgcca tcagtggccc ctattgttga ggcaatgaaa cggcttaatt taggggacgt
      841 ttctcgtgta gttcaggagg atgttgctct acaggaaccg caggccaaat caactacgcg
      901 aaagggtaaa aaacgacgca agaaggctat aacagaagat gtaaaggcgc aacggaccga
      961 agcagccgaa cacgctcgcg aaatgttcgg tcaaaaaggc ggtgcgcaaa aaagccaaaa
     1021 ccaacgcgat gcgcgtggtc gttttattgg aaagccaggg agtaaggccg cagcggaaga
     1081 tgcccgtgct gaacgtgctg aaaaggccag gcgcaaagag gatgatgagc gtctaaatgc
     1141 tgaatcaggt ttattaaaaa aactgtcaaa agtagctgaa gg
//
LOCUS       DAMZFT010000344         1106 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.347, whole genome shotgun sequence.
ACCESSION   DAMZFT010000344 DAMZFT010000000
VERSION     DAMZFT010000344.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1106)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1106)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1106
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.347"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            88..309
                     /locus_tag="PN977_005378"
     CDS             88..309
                     /locus_tag="PN977_005378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000245708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
                     /protein_id="HDI6045264.1"
                     /translation="MVNANPCARQEFIWRFYSCKKHHYHFVIAATEDEARSQLPDGPC
                     IFTARFSTNSRNSLSYWNLPFSSDVQGVL"
     gene            306..>1106
                     /locus_tag="PN977_005379"
     CDS             306..>1106
                     /locus_tag="PN977_005379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015974252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor Ant"
                     /protein_id="HDI6045265.1"
                     /translation="MKKPLVTRNDIAEAIALHTACMPTREIPGAIANYFMITRRFYTR
                     TDKAVINRLLIAEIRDYLIEQGRLRYATVAAEMRKEAHRMTSNNLNVKKPASFASATP
                     SPAVNVISNTGDTIDSQTLLKMVNEARKLCGEPEVRNNKFIKKILDELEGEDGYTKSA
                     TVPPGGGTPMVVITMTYKQALRVAARESKAVRRSLIDKLEELQQANSPAPSIPQTLPD
                     ALRLAAELAEQKMQLEQQLVAAAPKVDFADRVSVANGILIGNFAKVVGL"
BASE COUNT          311 a          293 c          255 g          247 t
ORIGIN      
        1 aatcgccagt ctgctaaccc cgtcacgtcc tgccacctgt ttagcagcgg gtagcaggtt
       61 gttaaacctg attgtgaggc cgtaactatg gttaatgcca atccttgcgc acgccaagaa
      121 ttcatctggc gtttctattc ctgcaaaaaa caccactatc acttcgtcat cgcagcaaca
      181 gaagacgaag cacgctctca attgcctgat ggcccctgca tttttactgc ccgtttttca
      241 actaactcgc gcaattcact tagttactgg aacctcccct tctcttccga cgttcagggg
      301 gttttatgaa aaaacctctc gtcacccgta atgacatagc cgaagcgatc gccctgcata
      361 ctgcctgtat gccgacacgg gagatccccg gcgcaattgc caactatttc atgataacca
      421 gacgttttta tacccgaaca gataaggctg tgatcaacag gctactgata gccgagatca
      481 gggattattt gattgaacaa ggacgtctac gttacgcaac ggtggcagca gaaatgagaa
      541 aggaggcaca tagaatgacc agtaataatt tgaatgttaa aaaaccagca tcttttgctt
      601 cagctacgcc atcaccagct gtgaatgtta tctccaacac tggagacaca atcgacagcc
      661 agacgctgtt aaagatggtc aatgaagcgc gtaagttatg tggggaacca gaggttcgga
      721 acaacaaatt catcaaaaaa atactcgacg aattagaagg tgaggacggt tacacaaaaa
      781 gtgcaaccgt gccgccaggt ggcggtacgc ctatggttgt cataaccatg acctacaaac
      841 aagccctgcg agtcgccgcg cgcgagtcaa aagcagtccg ccgttcgctg atcgacaaac
      901 tggaagaatt gcagcaggca aactcccctg ccccatcgat cccccaaaca ttaccagacg
      961 ctctacgcct ggctgccgag ttggcagaac agaaaatgca gttggaacaa cagctggtgg
     1021 ccgcagcccc taaagtcgat tttgccgacc gggtatcagt ggccaatgga atcctgatcg
     1081 ggaactttgc aaaggtcgtt ggactt
//
LOCUS       DAMZFT010000345         1106 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.348, whole genome shotgun sequence.
ACCESSION   DAMZFT010000345 DAMZFT010000000
VERSION     DAMZFT010000345.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1106)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1106)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1106
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.348"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..999
                     /gene="iucA"
                     /locus_tag="PN977_005380"
     CDS             <1..999
                     /gene="iucA"
                     /locus_tag="PN977_005380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001314127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobactin synthase IucA"
                     /protein_id="HDI6045266.1"
                     /translation="AKGLIRDLGEAGTSWLPTTSSRSLYCATSRDMIKFSLSVRLTNS
                     VRTLSVKEVERGMRLARLAQTDGWQMLQARFPTFRVMQEDGWAGLRDLNGNIMQESLF
                     SLRENLLLEQPQSQTNVLVSLTQAAPDGGDSLLVSAVKRLSDRLGITVQQAAHAWVDA
                     YCQQVLKPLFTAEADYGLVLLAHQQNILVQMLGDLPVGFIYRDCQGSAFMPHATEWLD
                     TIDEAQAENIFTREQLLRYFPYYLLVNSTFAVTAALGAAGLDSEANLMARVRTLLAEV
                     RDQVTHKTCLNYVLESPYWNVKGNFFCYLNDHNENTIVDPSVIYFDFANPLQAQEV"
     gene            1000..>1106
                     /locus_tag="PN977_005381"
     CDS             1000..>1106
                     /locus_tag="PN977_005381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HDI6045267.1"
                     /translation="MSGANIVHSGYGLRCEKLDKPLNLGWGLDNSAVLH"
BASE COUNT          228 a          295 c          317 g          266 t
ORIGIN      
        1 gctaaaggac ttatcagaga cttaggcgag gccggcacgt cgtggctgcc gaccacctct
       61 tcccgctccc tctactgtgc taccagccgc gatatgatca agttctccct gagcgttcgg
      121 ctgaccaact ccgtccgtac tctgtctgtg aaagaggtgg agcgaggaat gcgcctggca
      181 cgtctggcgc aaaccgacgg ctggcagatg ctacaggccc gcttccctac tttccgggta
      241 atgcaggagg acggctgggc cgggctgcgc gatcttaacg gcaacatcat gcaggaaagt
      301 ttgttttccc tgcgtgaaaa cctgctgctg gagcagccgc aaagccagac caacgttctg
      361 gtttccctga ctcaggccgc accggatggc ggtgattcgc tgctggtttc ggcggtaaaa
      421 cgcctgagcg atcgcctcgg tatcactgtg caacaggccg cccacgcatg ggtcgatgct
      481 tactgtcagc aggtgctgaa gcctctgttt acggctgaag cggattacgg cctggtgctg
      541 ctggcgcatc agcaaaatat tcttgtgcag atgcttgggg atctgccggt cggatttatt
      601 taccgtgact gccagggcag cgcgtttatg cctcacgcga cagagtggct cgataccatt
      661 gacgaggcgc aggcggaaaa tattttcacc cgtgagcagt tgctgcgcta tttcccttat
      721 tacctgctgg ttaactccac ttttgccgta actgctgcgc tgggtgctgc cgggctggac
      781 agcgaagcga atctgatggc tcgtgtacga acattgctgg ctgaagtgcg tgaccaggtg
      841 actcataaaa cctgtctcaa ctatgttctg gaaagtccgt actggaacgt aaaaggtaac
      901 tttttctgtt atctgaacga tcataacgag aacaccatcg ttgacccttc ggtgatctat
      961 tttgatttcg ctaacccgct gcaggctcag gaggtctgaa tgtctggggc aaacattgtt
     1021 cacagcggat atggactgcg ctgtgaaaaa ctcgacaagc ctctgaatct tggctggggg
     1081 ctggacaata gcgcggtgtt gcactg
//
LOCUS       DAMZFT010000346         1074 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.350, whole genome shotgun sequence.
ACCESSION   DAMZFT010000346 DAMZFT010000000
VERSION     DAMZFT010000346.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1074)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1074)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1074
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.350"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..437
                     /gene="dgoA"
                     /locus_tag="PN977_005382"
     CDS             <1..437
                     /gene="dgoA"
                     /locus_tag="PN977_005382"
                     /EC_number="4.1.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-6-phosphogalactonate aldolase"
                     /protein_id="HDI6045268.1"
                     /translation="IGAGTVLKPEQVDVLARMGCQLIVTPNIHSEVIRRAVGYGMTVC
                     PGCATATEAFTALDAGAQALKIFPSSAFGPQYIKALKAVLPPDIAVFAVGGVTPENLA
                     QWIDAGCAGAGLGSDLYRAGQSVERTAQQAAAFVKAYREAVQ"
     gene            434..>1074
                     /gene="dgoD"
                     /locus_tag="PN977_005383"
     CDS             434..>1074
                     /gene="dgoD"
                     /locus_tag="PN977_005383"
                     /EC_number="4.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020077623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactonate dehydratase"
                     /protein_id="HDI6045269.1"
                     /translation="MKITKITTYRLPPRWMFLKIETDEGVVGWGEPVIEGRARTVEAA
                     VHELGDYLIGQDPSRINDLWQVMYRAGFYRGGPILMSAIAGIDQALWDIKGKVLNSPV
                     WQLMGGLVRDKIKAYSWVGGDRPANVIDGIKILREIGFDTFKLNGCEELGLIDNSRAV
                     DAAVNTVAQIREAFGNQIEFGLDFHGRVSAPMAKVLIKELEPYRPLFIEEPVLA"
BASE COUNT          244 a          266 c          328 g          236 t
ORIGIN      
        1 tgattggcgc aggtacggta ctgaaacctg aacaggtcga tgtgctcgcc aggatgggct
       61 gccagctcat cgttacgccc aatatccata gtgaagtgat ccgccgtgcg gtgggctacg
      121 gcatgaccgt ttgccccggc tgcgcgacgg cgaccgaagc ctttaccgcg ctcgacgcgg
      181 gcgctcaggc gctgaaaata tttccgtcat cggcttttgg tccgcaatac atcaaagcgt
      241 taaaagcggt attaccaccg gacatcgcag tctttgccgt tggcggcgtg acgccagaaa
      301 acctggcgca gtggatagac gcaggttgtg caggggcagg cttaggcagc gatctctatc
      361 gcgccgggca gtccgtagaa cgcaccgcgc agcaggcagc agcatttgtt aaggcgtatc
      421 gagaggcagt gcaatgaaaa tcaccaaaat taccacgtat cgtttacctc cccgctggat
      481 gttcctgaaa attgaaaccg atgaaggcgt ggtcggctgg ggcgagcccg tgatcgaagg
      541 ccgcgcccgt acggtggaag cggcagttca cgagctgggt gactatttga ttggtcagga
      601 tccatcgcgc atcaatgact tatggcaagt gatgtatcgc gccggatttt atcgtggagg
      661 tccaatcctg atgagcgcca ttgccgggat cgaccaggcg ttatgggata tcaaaggcaa
      721 agtgctgaat tcgccggtct ggcaactgat gggcggcctg gtgcgtgaca aaattaaagc
      781 ctacagttgg gtcggcggcg atcgtccggc gaatgttatc gacggaataa aaatcctgcg
      841 cgaaatcggt tttgatacct tcaaactgaa tggttgtgaa gaactggggc taattgataa
      901 ctcccgcgcg gtagatgcgg cggtcaacac cgtggcacaa attcgtgaag cttttggcaa
      961 tcagattgag tttggtcttg atttccacgg tcgcgtcagc gcgccgatgg cgaaagtact
     1021 gattaaagaa ctggaaccgt atcgcccgct gtttattgaa gagccggtgc tggc
//
LOCUS       DAMZFT010000347         1066 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.351, whole genome shotgun sequence.
ACCESSION   DAMZFT010000347 DAMZFT010000000
VERSION     DAMZFT010000347.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1066)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1066)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1066
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.351"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            112..792
                     /locus_tag="PN977_005384"
     CDS             112..792
                     /locus_tag="PN977_005384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001290017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ead/Ea22-like family protein"
                     /protein_id="HDI6045270.1"
                     /translation="MTIDYQVLREAAEKATPDEWVAFISTDTGTYAVHTPGDERCEDV
                     IKWTGFDGQKNAENNARYVAAFNPKVALELLGEIKRLEDTNIDAMCRIAELETNLAAL
                     VAENAGLKAFKTAVYQQMGVGCDAPEFSITVGLSNLRRFADTLHAIEREFFTKELPDE
                     EHEGETFNECPLSWGMSVEQYVSEFRKCLAEVRAQGVEMFAECAYTLEHHDHAVAFAA
                     ELRKGGNQ"
     gene            789..>1066
                     /locus_tag="PN977_005385"
     CDS             789..>1066
                     /locus_tag="PN977_005385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001289896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ead/Ea22-like family protein"
                     /protein_id="HDI6045271.1"
                     /translation="MSEINYQALREAAERATPAMERLLMLPVDDDLLSEQELKDYGVD
                     IDALNAFKFLAGPETVLALLDEREADKKRIAELEAREIKPAKGEVLVVV"
BASE COUNT          294 a          223 c          305 g          244 t
ORIGIN      
        1 gcgaagcggg aaggtgcgtt ggaattgtcc gcattgcgga aaaataacct ggcagtacag
       61 cccatatgat tttgaaggct gtagtgatta caactgtgaa ggatggcgag aatgacaatt
      121 gactatcagg tactgcgtga ggcggcagaa aaggcaacac cagacgaatg ggtcgcattt
      181 atttcgacgg atactggtac ttatgcggtg cacacgcccg gtgatgaacg atgtgaagac
      241 gttatcaaat ggaccggctt tgatggacag aaaaatgcag agaacaacgc tcgttatgtt
      301 gccgcgttca acccaaaggt tgcactggag ctgcttggtg aaattaagcg cctggaggac
      361 acaaatattg atgctatgtg ccgaattgca gagcttgaga ctaatctcgc ggcgctggtg
      421 gcggagaatg cggggcttaa ggccttcaaa accgccgttt atcagcagat gggcgtcgga
      481 tgtgatgctc ctgaattctc cattacggta ggtttgagta acttacgtcg ttttgctgac
      541 acactccacg ccattgagcg tgagttcttt accaaagagc tacctgatga agagcacgaa
      601 ggcgaaacat tcaatgaatg tccacttagc tggggaatga gcgtcgaaca gtacgtttcc
      661 gagtttcgca aatgcctggc tgaagtacgg gcgcagggcg tggagatgtt tgcggagtgt
      721 gcatacacac ttgaacatca tgatcacgca gtagccttcg ccgctgagct tcgcaaagga
      781 ggcaaccagt gagcgaaatt aattaccagg cactgcgtga ggcggcagaa cgtgcaactc
      841 cagcaatgga acgcctgtta atgttgccag ttgatgatga tttgttaagt gaacaggaac
      901 ttaaagatta cggtgtggat attgatgcgc tcaacgcctt caaatttctg gccggaccag
      961 aaaccgtgct ggcactactt gatgaacggg aagcagacaa aaagcgcatt gcagaactgg
     1021 aagctcggga aataaaacca gccaaaggtg aagttcttgt cgttgt
//
LOCUS       DAMZFT010000348         1047 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.352, whole genome shotgun sequence.
ACCESSION   DAMZFT010000348 DAMZFT010000000
VERSION     DAMZFT010000348.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1047)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1047)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1047
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.352"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..1038
                     /locus_tag="PN977_005386"
     rRNA            <1..1038
                     /locus_tag="PN977_005386"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          246 a          241 c          330 g          230 t
ORIGIN      
        1 ggtaacgcga agctcttgat cgaagccccg gtaaacggcg gccgtaacta taacggtcct
       61 aaggtagcga aattccttgt cgggtaagtt ccgacctgca cgaatggcgt aatgatggcc
      121 aggctgtctc cacccgagac tcagtgaaat tgaactcgct gtgaagatgc agtgtacccg
      181 cggcaagacg gaaagacccc gtgaaccttt actatagctt gacactgaac attgagcctt
      241 gatgtgtagg ataggtggga ggctttgaag tgtggacgcc agtctgcatg gagccggcct
      301 tgaaatacca ccctttaatg tttgatgttc taacgttggc ccctgaccgg ggttgcggac
      361 agtgtctggt gggtagtttg actggggcgg tctcctccta aagagtaacg gaggagcacg
      421 aaggttggct aatcctggtc ggacatcagg aggttagtgc aatggcataa gccagcttga
      481 ctgcgagcgt gacggcgcga gcaggtgcga aagcaggtca tagtgatccg gtggttctga
      541 atggaagggc catcgctcaa cggataaaag gtactccggg gataacaggc tgataccgcc
      601 caagagttca tatcgacggc ggtgtttggc acctcgatgt cggctcatca catcctgggg
      661 ctgaagtagg tcccaagggt atggctgttc gccatttaaa gtggtacgcg agctgggttt
      721 agaacgtcgt gagacagttc ggtccctatc tgccgtgggc gctggagaac tgaggggggc
      781 tgctcctagt acgagaggac cggagtggac gcatcactgg tgttcgggtt gtcatgccaa
      841 tggcactgcc cggtagctaa atgcggaaga gataagtgct gaaagcatct aagcacgaaa
      901 cttgccccga gatgagttct ccctgactcc ttgagagtcc tgaaggaacg ttgaagacga
      961 cgacgttgat aggccgggtg tgtaagcgca gcgatgcgtt gagctaaccg gtactaatga
     1021 accgtgaggc ttaaccttac aacgccg
//
LOCUS       DAMZFT010000349         1046 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.353, whole genome shotgun sequence.
ACCESSION   DAMZFT010000349 DAMZFT010000000
VERSION     DAMZFT010000349.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1046)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1046)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1046
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.353"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..220
                     /locus_tag="PN977_005387"
     CDS             <1..220
                     /locus_tag="PN977_005387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001354498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDI6045272.1"
                     /translation="KGFERGIAQGMERGKLEGRMEGRMEGRAEGRLEGQLEGKLETAR
                     NMLAEGMGLQTIMKITGLSEEQLKKISH"
     gene            694..1014
                     /locus_tag="PN977_005388"
                     /pseudo
     CDS             694..1014
                     /locus_tag="PN977_005388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001546148.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          268 a          242 c          300 g          236 t
ORIGIN      
        1 caagggattt gagcgcggta ttgcccaggg tatggagcgc ggtaagctag aaggcagaat
       61 ggaaggcaga atggaaggcc gtgccgaagg gcgactcgaa ggacaactgg aaggcaaact
      121 ggaaacggcg cgtaatatgt tggctgaagg aatggggctt cagaccatca tgaaaatcac
      181 gggattgagt gaagaacagc tgaaaaaaat cagccactga agcattgagg cgtgatgtat
      241 agcacgcctc gttctgtctg acagtatttt gtttcctgtg tatttggtcg tttcaggtca
      301 acctttgtcg ggagcctggc gttggtacct gctgaggaaa gagggaactg gacgcagagt
      361 gacagtctgc gaattgcagg aagaggatgc gaaagtgttc gcttcttcca ttgtgtccct
      421 gatttaaatt cggatactca aatctgaagc ggtgactcgt atacgtgtga gtcaccggga
      481 ctaccgcttg agtgatgaaa aatagtcgtg ttaacgcaaa gctgaagtga cacatcttat
      541 ttcagcgaat gcgtttgacc gccttactat acacttttga gcattctctt ttcctgacgg
      601 aaaccacgaa gacgacaatc ttctcgtcta taacctgata tacaaggcaa aaaaccgtca
      661 agagcaggag caggcggtcc gccagacagt ccattgcata aggctatcag ctatatcctg
      721 aaacgacctg atgagctgtg ccgttttctg gatgatggct ctgtgccacc ggacaacaat
      781 atctgcgaac gagccatccg cccggtcacg ctggggcgaa aggcatggtt attcgcaggc
      841 tcgctgatgg ccggcaaacg cgcggcacag ataatgagtc ttctggaaac agcaaagcgt
      901 aatggtctgg agccacatgc ctggctgacg gacgtactga cgcgcctgcc ggagtggcct
      961 gaggaccgac tggctgagct gctgcctctt gagggattta ccttctccgg gtgacacaac
     1021 acctgccgtc aggtctttgc acaccg
//
LOCUS       DAMZFT010000350         1037 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.354, whole genome shotgun sequence.
ACCESSION   DAMZFT010000350 DAMZFT010000000
VERSION     DAMZFT010000350.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1037)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1037)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1037
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.354"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            220..579
                     /locus_tag="PN977_005389"
     CDS             220..579
                     /locus_tag="PN977_005389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding transcriptional regulator"
                     /protein_id="HDI6045273.1"
                     /translation="MFKERMTPDELARLTGYSRQTINKWVRKEGWTTSPKPGVQGGKA
                     RLVHVNEQVREYIRNAERPESQGETPALSGDAPLEVLLVTLAKEMTPVEQKQFTSLLL
                     REGIIGLLQRLGIRDSK"
     gene            581..973
                     /locus_tag="PN977_005390"
     CDS             581..973
                     /locus_tag="PN977_005390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416898.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding transcriptional regulator"
                     /protein_id="HDI6045274.1"
                     /translation="MKRLRNKMTTEELAECLGVAKQTVNRWIREKGWKTEKFPGVKGG
                     RARLILVDTQVCEFIQNTPAFHNTPMLLEAEEPLAEYAPGIRTPAYRQIISAIDNMTH
                     SEREKVAQFLSREGIRNFLARLDIDESA"
BASE COUNT          304 a          238 c          257 g          238 t
ORIGIN      
        1 tcacgtaaag cttgtcgagt tgacgggcgg catcatatga attccgcccg aatgtgtcaa
       61 cggcaaattg caacgtgtag tttcaatcgc tgaaaaatca ggcaaatgaa caattttgga
      121 aaaccgctcg cattcagtag ttattcatgt cacggtttcc tgtaaagtgg tgttataaaa
      181 tgaactacta atagacccac atacattcag ggaattgtta tgttcaagga gaggatgacg
      241 ccagatgaac ttgccagact gaccggctat agccgccaga ccattaataa atgggtacgc
      301 aaggaaggct ggacaacgtc accaaaacca ggcgtccagg gtggcaaagc ccgactggtt
      361 cacgtcaatg aacaggttcg tgaatatatt cgcaatgctg aacgtccgga aagtcaggga
      421 gaaacgcctg ccctgtctgg tgatgcgccg cttgaagtgt tgctggtgac actggcgaaa
      481 gagatgacgc cagttgaaca aaaacagttt acatccttgc ttctgcggga agggattatc
      541 ggattattac aacgcttagg gattcgcgat agcaaataat atgaaaagat tacgcaataa
      601 aatgaccacc gaagaactgg ctgaatgcct cggtgtggct aaacaaactg ttaaccgttg
      661 gatcagagaa aaaggctgga aaacggaaaa atttcccggc gtgaaaggcg gtcgtgccag
      721 acttattctg gtcgatacgc aagtttgcga gtttattcag aacacgccag ccttccataa
      781 cacgccgatg cttctggaag ccgaagaacc ccttgccgaa tacgcaccgg ggattcgcac
      841 tcctgcttat cggcaaatca tcagcgcaat agacaacatg actcacagtg agcgggaaaa
      901 agttgcgcaa tttttgtcac gcgaaggaat tcgtaatttc cttgctcgtc tcgacataga
      961 tgaatcagct tgacatcctc cacgccctga aggacgagga tccctgctac gttcaggctg
     1021 tcgcctgaat catttcg
//
LOCUS       DAMZFT010000351          986 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.355, whole genome shotgun sequence.
ACCESSION   DAMZFT010000351 DAMZFT010000000
VERSION     DAMZFT010000351.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 986)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 986)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..986
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.355"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            35..535
                     /locus_tag="PN977_005391"
     CDS             35..535
                     /locus_tag="PN977_005391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001405508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ogr/Delta-like zinc finger family protein"
                     /protein_id="HDI6045275.1"
                     /translation="MIYCPSCGHVAHTRRAHFMDDGTKIMIAQCRNIYCSATFEASES
                     FFSDSKDSGMEYISGKQRYRDSLTSASGSMKRPKRMLVTGYCCRRCKGLALSRTSRRL
                     SQEVTERFYVCTDPGCGLVFKTLQTINRFIVRPVTPDELAESLHEKQELPPVRLKTQS
                     YSLRLE"
     gene            609..>986
                     /locus_tag="PN977_005392"
     CDS             609..>986
                     /locus_tag="PN977_005392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Polarity suppression protein"
                     /protein_id="HDI6045276.1"
                     /translation="MESTALQQAFDTCQNNKAAWLQRKNELAAAEQEYLRLLSGEGRN
                     VSRLDELRNIIEVRKWQVNQAAGRYIRSHEAVQHISIRDRLNDFMQQHGTALAAALAP
                     ELMGYSELTAIARNCAIQRATDAL"
BASE COUNT          262 a          248 c          264 g          212 t
ORIGIN      
        1 agggtgcaga gcttaaggcc agaggactgc tgtcatgatt tactgcccgt cgtgtggaca
       61 tgttgctcac acccgccgcg cacatttcat ggacgatggc accaagataa tgattgcaca
      121 gtgccggaat atttattgct ctgcgacatt tgaagcgagt gaaagctttt tctctgacag
      181 taaagattca ggaatggaat acatttcagg caaacagaga taccgcgatt cactgacgtc
      241 agcctccggc agtatgaaac gcccgaaaag aatgcttgtt accggatatt gttgtcggag
      301 atgtaaaggc cttgcactgt caagaacatc gcggcgtctg tctcaggaag tcaccgagcg
      361 tttttatgtg tgcacggatc cgggctgtgg tctggtgttt aaaacgcttc agaccatcaa
      421 ccgtttcatt gtccgcccgg tcacgccgga cgaactggca gaaagcctgc atgaaaaaca
      481 ggaactgccg ccagtacgct taaaaacaca atcatattcg ctgcgtctgg aatgagggct
      541 gccggttaac accggccgtc gccgcacacc gtatttttat tcttcagcat gatgagaaag
      601 agataacgat ggaaagcaca gccttacagc aggcctttga cacctgtcag aataacaaag
      661 cagcatggct gcaacgcaaa aatgagctgg cagcggccga acaggaatac ctgcggcttc
      721 tgtcaggaga aggcagaaac gtcagtcgcc tggacgaatt acgcaatatt atcgaagtca
      781 gaaaatggca ggtgaatcag gccgccggtc gttatattcg ttcgcatgaa gccgttcagc
      841 acatcagcat ccgcgaccgg ctgaatgatt ttatgcagca gcacggcaca gcactggcgg
      901 cggcactggc accggagctg atgggctaca gtgagctgac ggccattgcc cgaaactgtg
      961 ccatacagcg tgccacagat gccctg
//
LOCUS       DAMZFT010000352          965 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.356, whole genome shotgun sequence.
ACCESSION   DAMZFT010000352 DAMZFT010000000
VERSION     DAMZFT010000352.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 965)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 965)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..965
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.356"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(<1..321)
                     /gene="sitD"
                     /locus_tag="PN977_005393"
     CDS             complement(<1..321)
                     /gene="sitD"
                     /locus_tag="PN977_005393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter permease subunit
                     SitD"
                     /protein_id="HDI6045277.1"
                     /translation="MMALLLEPLQFTFMSHALLISLVVSIPCALLSVFLVLKSWALMG
                     DAMSHAVFPGIVLAWILGLPLATGAFVAGVFCAVATGYLKDNSRIKQDTVMGIVFSGM
                     FAAGL"
     gene            complement(318..>965)
                     /gene="sitC"
                     /locus_tag="PN977_005394"
     CDS             complement(318..>965)
                     /gene="sitC"
                     /locus_tag="PN977_005394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter permease subunit
                     SitC"
                     /protein_id="HDI6045278.1"
                     /translation="SGGLAAGSMLFLNQLTRLKEDAIIGLIFSSFFGLGLFMVSLNPT
                     SVNIQTIVLGNILAIDPADILQLTIIGILSIIVLFFKWKDLMVTFFDENHARAIGLHP
                     GRLKILFFTLLSVSTVAALQTVGAFLVICLVVTPGATAWLLTDRFPRLLMIAVTIGSV
                     TSFLGAWVSYFLDGATGGIIVVAQTLLFLLAFVFAPTHGLLANRRRAHKALEDRS"
BASE COUNT          288 a          261 c          241 g          175 t
ORIGIN      
        1 cagacctgcg gcaaacatgc cagaaaaaac aatccccatc actgtatctt gcttgattcg
       61 gctgttgtct ttcaggtatc ccgtcgccac cgcacagaat actccggcga cgaatgcccc
      121 tgtagccaga ggtaacccca gaatccaggc aagcacgatg ccaggaaaaa ccgcatgact
      181 catcgcgtca cccatcagtg cccaactttt caggaccaga aagaccgaca gcagcgcaca
      241 gggtatggaa acaaccagtg aaatcagcag tgcgtgactc ataaaggtga actgcagtgg
      301 ctccagtaat aatgccatca tgagcgatcc tccagcgctt tatgcgcacg ccgacgattt
      361 gccaaaagcc catgggtggg cgcaaagaca aatgctaaaa gaaacagcag tgtctgcgcc
      421 accacgatga ttccacctgt cgcgccgtcc agaaagtaac tgacccacgc gccgaggaaa
      481 ctggtcacac tgccaatagt tacagcaatc atgagcaggc ggggaaaacg atcggttaac
      541 aaccatgctg ttgcaccggg agtcaccacc agacagatca ccagaaaggc accaaccgtt
      601 tgcagcgccg caacggtcga aaccgacagc agggtgaaga agaggatttt taatctgccc
      661 ggatgcaatc caatagcacg ggcgtgattc tcgtcgaaaa aggtcaccat aagatctttc
      721 catttgaaaa aaagcacgat gatcgagaga atgccaatga tagtgagttg caggatatcc
      781 gccggatcga ttgccagaat attgcccagg acgatggtct gaatgttcac ggatgtcggg
      841 ttcaatgaca ccataaacag ccccaggcca aaaaaggacg agaagatcag gccgatgatg
      901 gcatcttctt ttaagcgagt gagttggtta agaaacagca tgctgcccgc tgccagtccg
      961 ccgga
//
LOCUS       DAMZFT010000353          956 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.357, whole genome shotgun sequence.
ACCESSION   DAMZFT010000353 DAMZFT010000000
VERSION     DAMZFT010000353.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 956)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 956)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..956
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.357"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(<1..911)
                     /locus_tag="PN977_005395"
     CDS             complement(<1..911)
                     /locus_tag="PN977_005395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS4-like element IS4 family transposase"
                     /protein_id="HDI6045279.1"
                     /translation="MFPDFFMHIGQALDLVSRYDSLRNPLTSLGDYLDPELISRCLAE
                     SGTVTLRKRRLPLEMMVWCIVGMALERKEPLHQIVNRLDIMLPGNRPFVAPSAVIQAR
                     QRLGSEAVRRVFTKTAQLWHNATPHPHWCGLTLLAIDGVFWRTPDTPENDAAFPRQTH
                     AGNPALYPQVKMVCQMELTSHLLTAAAFGTMKNSENELAEQLIEQTGDNTLTLMDKGY
                     YSLGLLNAWSLAGEHRHWMIPLRKGAQYEEIRKLGKGDHLVKLKTSPQARKKWPGLGN
                     EVTARLLTVTRKGKVCHLLTSMTDAMR"
BASE COUNT          199 a          258 c          272 g          227 t
ORIGIN      
        1 aagcgcatgg cgtccgtcat cgacgtcagc agatggcaga cttttccttt gcgcgtcacg
       61 gtcagcaggc gggctgtcac ttcatttccc agtcccggcc acttttttcg tgcctgcggg
      121 ctggttttca gcttcaccag atgatcgcct ttacccagtt ttctgatctc ttcatattgc
      181 gctccctttc tgagaggtat catccagtgg cggtgttctc ccgccaggct ccaggcattt
      241 aacagtccca gtgagtaata acctttatcc attaacgtca gagtgttatc gccggtttgt
      301 tctataagtt gctcagcaag ctcattttcg ctgttcttca tcgtgccgaa ggctgcagcc
      361 gtcagcagat ggctggtcag ttccatctgg cagaccattt tgacctgcgg gtagagcgcc
      421 gggttcccgg catgtgtctg gcgggggaag gctgcatcgt tctctggtgt atccggtgtg
      481 cgccagaaca caccatcgat ggccagcagg gtcaggccgc accagtgcgg atgcggcgtg
      541 gcgttatgcc agagctgcgc tgttttcgtg aacacgcggc ggacagcctc acttcccagg
      601 cgctggcggg cctgaataac ggcactgggg gcaacgaagg ggcgattgcc cggcagcatg
      661 atgtccaggc gattcacaat ctggtgaaga ggttctttac gctcaagcgc catgccaaca
      721 atacaccaga ccatcatttc gaggggaaga cggcgcttgc gtagcgttac agtacctgat
      781 tcggcaaggc aacgagagat gagttcgggg tcgaggtaat cccccagaga agtcagtggg
      841 ttacgcagag aatcgtaacg ggataccaga tcaagagcct gtccaatgtg cataaaaaaa
      901 tccggaaaca agtgagcgtt tccggattct tacacagcca ctggatcggt caactg
//
LOCUS       DAMZFT010000354          956 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.358, whole genome shotgun sequence.
ACCESSION   DAMZFT010000354 DAMZFT010000000
VERSION     DAMZFT010000354.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 956)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 956)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..956
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.358"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..659
                     /gene="rsmC"
                     /locus_tag="PN977_005396"
     CDS             <1..659
                     /gene="rsmC"
                     /locus_tag="PN977_005396"
                     /EC_number="2.1.1.172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="16S rRNA (guanine(1207)-N(2))-methyltransferase
                     RsmC"
                     /protein_id="HDI6045280.1"
                     /translation="ADYAPLNKVDSARRCGLYFGRLEKQPVFDANKFWGEYNVDGLTV
                     KTLPGVFSRDGLDVGSQLLLSTLTPHTKGKVLDVGCGAGVLSVAFARHSPKIRLTLCD
                     VSAPAVEASRATLAANGIEGEVFASNVFSEVKGRFDMIISNPPFHDGMQTSLDAAQTL
                     IRGAVRHLNSGGELRIVANAFLPYPDVLDETFGFHEVIAQTGRFKVYRAIMTRQAKKG
                     "
BASE COUNT          212 a          258 c          259 g          227 t
ORIGIN      
        1 ttgcagatta tgcaccgttg aataaggtgg acagcgcccg tcgctgtggc ctctattttg
       61 gtcgtctgga aaaacagccg gtatttgatg ccaataaatt ctggggcgaa tataacgtcg
      121 atggcctgac ggtcaaaacg ctgcctggcg tgtttagccg cgacggtctg gatgtcggta
      181 gccagttgct gctctcgacg ttaaccccgc acacgaaagg taaagtgctg gatgtcggct
      241 gtggcgcggg cgtactttcg gttgcctttg cgcgccactc accgaagatt cgtctcacgt
      301 tgtgcgatgt ttctgcgcca gcagttgaag ccagccgtgc aacacttgcg gccaacggta
      361 ttgaaggtga agtctttgcc agcaacgtct tttctgaggt gaaaggtcgt tttgatatga
      421 tcatctccaa cccgccgttc cacgatggga tgcaaaccag cctcgatgcg gcgcaaacgc
      481 tgattcgcgg cgcggtgcgt catcttaata gcggcggcga gctgcgaatt gtagcgaacg
      541 ccttcctgcc ttacccggac gtgctggatg agacatttgg cttccacgaa gtgatcgcgc
      601 aaaccgggcg cttcaaggtg tatcgcgcca ttatgacccg ccaggcgaag aaaggttgag
      661 tatcccgccg caagccttgc cggatgcggc gtgctcgcct tatacaacct acaaaacctg
      721 caagttagcc cttcgtaggc cagataagac gcgcccgcgt cgcatctggc atctgcaccc
      781 aaataccgtg tcggatgcgg cgtgaacgcc ttatccgacc tacaaaacct gcacgttagt
      841 ttcctgatgg acatttttcc agcaattaca cctccggcga taattaacta ttgacgaaaa
      901 gctgaaaacc actagaatgc gcctccgtgg tagcaattct ttttaagaat tgatgg
//
LOCUS       DAMZFT010000355          934 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.359, whole genome shotgun sequence.
ACCESSION   DAMZFT010000355 DAMZFT010000000
VERSION     DAMZFT010000355.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 934)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 934)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..934
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.359"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            160..>934
                     /locus_tag="PN977_005397"
     CDS             160..>934
                     /locus_tag="PN977_005397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001000782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber protein"
                     /protein_id="HDI6045281.1"
                     /translation="MNDVTVVTSVTYPSPESLALVADVQYHEPYLSAALNRKFRGIVD
                     PGFYAGFLPKPGGGMNLLITSVDGDKTAGAASVDIGEFYQVTIQHRKDISLALNAGKK
                     YAIVLKGRYLLGEDTYQVNTASHIHAAEFVARTYTDSYQLGDGELLVCTVNIPAGVST
                     ITQEMIDTSERINRTIGIDISDSVTSTRSDVAASSLAVKKAYDLAKSKYTAQDASTTQ
                     KGLVQLSSATNSTSEVLAATPKAVKAAYDLANGKYTAQDA"
BASE COUNT          266 a          208 c          229 g          231 t
ORIGIN      
        1 gcaggaaggg aaaaactacc cattcaacac tctggttgtt ctggataacg agaataagcc
       61 aatcgccatt atttgtgtcc aagaagactc gctgtatgtg ggcaaaacat ataccgcagt
      121 tatggccata aactcgacta cagcataagg atatgcttga tgaatgacgt tacagttgtt
      181 acatcggtta cttacccatc acccgagtcg ttggctctgg tggctgatgt gcaataccac
      241 gaaccatatc tgtcagccgc tctaaaccga aaattcaggg gaattgttga cccgggattt
      301 tatgctggtt tcttgcctaa gcctggcggt ggaatgaacc tgttaatcac ctcagtggat
      361 ggagataaaa ccgctggcgc ggcgtcagtg gatattggtg aattctacca ggtaactatt
      421 cagcatcgta aggatatctc tcttgcactt aacgcaggca agaaatatgc aattgtgctg
      481 aagggaagat accttcttgg agaagatacc tatcaggtga ataccgcgtc acatattcat
      541 gcagctgaat ttgttgccag aacctatacc gattcatatc agttaggtga tggggaactg
      601 ctggtttgta cggtgaatat ccctgctggc gtatctacca ttactcaaga gatgattgat
      661 acatccgagc gtatcaaccg cacgatcggc attgatattt cagactctgt aaccagtacc
      721 agaagtgatg ttgctgcgag ttcgctggca gttaaaaaag cctacgatct ggcgaaaagc
      781 aagtatacgg cgcaggatgc aagcacaacg caaaagggat tagttcagct cagtagtgcc
      841 actaacagta cgtccgaagt gctggccgcc acaccgaaag ctgtcaaggc tgcatatgac
      901 ctggctaacg ggaagtatac agcccaggat gcaa
//
LOCUS       DAMZFT010000356          889 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.360, whole genome shotgun sequence.
ACCESSION   DAMZFT010000356 DAMZFT010000000
VERSION     DAMZFT010000356.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 889)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 889)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..889
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.360"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(197..652)
                     /locus_tag="PN977_005398"
     CDS             complement(197..652)
                     /locus_tag="PN977_005398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000884152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HDI6045282.1"
                     /translation="MLDIILATRDIYFEKALREVFGTVSSRIRYIEDAISDRRRVQSG
                     RSFRYYFVFCDDEYTDIVRILFTGEACCILNKSMLNLRAAGGVLTLEERRAVLNRYYR
                     GLSIAEITEETGQNDKTVYGHLRRALQRSGFRKGRFIKGREAAGDSGVE"
     gene            complement(714..>889)
                     /locus_tag="PN977_005399"
     CDS             complement(714..>889)
                     /locus_tag="PN977_005399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000919997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YadA-like family protein"
                     /protein_id="HDI6045283.1"
                     /translation="PYSVGKFNATAAVGGYRDEQAIAVGVGYRFTENVAGKVAVAAGG
                     SSASWNAGVNFEF"
BASE COUNT          257 a          273 c          133 g          226 t
ORIGIN      
        1 caaccagtac aaataataaa agacatatca cagcaacact ccacactgac agcgcaaaaa
       61 taaatgccgc ctttgttatc ttcatataca agcaacctgt cctgataatc accaactcat
      121 gttttattct taaccattcg tcacaaatac aacaaaaaat aacagcagca gcaccgatat
      181 cacaatataa gatacattat tcaacacctg aatcccccgc cgcttctctc ccctttataa
      241 aacgcccctt ccggaatcca gaccgctgca gtgcacgacg aagatgaccg tataccgtct
      301 tgtcattctg ccccgtctct tctgtaatct ccgcaatact taaacctctg taataccggt
      361 tcagtaccgc tctgcgctct tccagcgtca acacacctcc ggccgctctc agattcagca
      421 tcgacttatt cagaatacag caggcctctc cggtaaaaag aatacgaaca atatctgtat
      481 actcatcatc acagaataca aaataatatc tgaatgacct gcctgactgc acccttcttc
      541 tgtctgatat ggcatcttct atatatctga ttcgactgct tacggttcca aatacctctc
      601 gcagggcttt ttcaaaataa atatctctcg tcgccaggat tatatccagc atctctgtac
      661 ctcattatat cccgggggac tccccccggg acagatatct aaatcctgac ctgtcagaac
      721 tcaaaattca caccagcatt ccacgatgcg gatgacccac ctgcagcaac tgcaactttt
      781 cctgccacat tctctgtgaa acggtagccc acgcccaccg caatggcctg ctcatcacgg
      841 taaccaccaa cggctgctgt cgcgttaaat tttccaacac tgtatggct
//
LOCUS       DAMZFT010000357          870 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.361, whole genome shotgun sequence.
ACCESSION   DAMZFT010000357 DAMZFT010000000
VERSION     DAMZFT010000357.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 870)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 870)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..870
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.361"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..531
                     /locus_tag="PN977_005400"
     CDS             <1..531
                     /locus_tag="PN977_005400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HDI6045284.1"
                     /translation="EDGENGLSPFVRTSIYRQSKHIRELEEQVKQVEEALASWYRTQE
                     ACQRMAKIPGVGMLTATYVVAAVGNARQFSTAKQFASWLGLTPKEHSSGGKQQLGGIS
                     KRGDGYFRYLLVHGARALTARVNRNGAVEKNSWLQGLLERKHYNVAVVAMAAKTARIM
                     WSMLSHNTEYQPRQLA"
BASE COUNT          246 a          207 c          245 g          172 t
ORIGIN      
        1 gaggatggcg aaaatggttt atctcccttt gtcagaacca gcatctacag acagtctaaa
       61 catatccggg aacttgaaga acaagttaaa caggtagaag aagctctggc ctcctggtat
      121 agaacgcagg aagcctgcca gagaatggcc aagatcccgg gggttggcat gctaacggcc
      181 acttatgtgg tagcagcagt gggtaatgcc cgacaattca gtaccgcaaa gcagttcgct
      241 tcatggctgg ggctgacacc aaaggaacat tccagcggcg ggaaacagca actgggaggg
      301 atcagcaaac gtggagatgg atatttccga tacctgctgg ttcacggcgc acgcgcactt
      361 accgccaggg tcaaccgaaa cggcgcggtt gagaagaatt cctggcttca gggactcctt
      421 gagcggaagc actacaatgt agctgttgtc gccatggcgg caaaaacagc gaggatcatg
      481 tggtcaatgt tgtcacacaa tactgaatat caacctcggc agctcgcctg acacaaggtc
      541 agtcaatctg acgaggtgct gaaaaaaagt taacgtcctg gtaggtgcag gtgacttcag
      601 aagatggcaa aacaggtaag accgcaggca ggatacaccg agaggtaaca agggcaagtt
      661 agcccgtaga ggatgattgg tacctgcctg gcggatttca tcatggttcg ggataaaaat
      721 cccatcagaa accggatata tggctgcacc tacccgtttt attacataac tgaagaaagc
      781 attgcattcc gggagcgtcc atatatgtta cctcgcgccc gacccggtag ccgcaaggga
      841 taagccgagg gtaccgcgca gcggcgattt
//
LOCUS       DAMZFT010000358          862 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.362, whole genome shotgun sequence.
ACCESSION   DAMZFT010000358 DAMZFT010000000
VERSION     DAMZFT010000358.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 862)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 862)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..862
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.362"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            18..862
                     /locus_tag="PN977_005401"
                     /pseudo
     CDS             18..862
                     /locus_tag="PN977_005401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558238.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     misc_feature    257..373
                     /locus_tag="PN977_005401"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01497"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="AL1L pseudoknot; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /pseudo
                     /db_xref="RFAM:RF01497"
BASE COUNT          197 a          206 c          265 g          194 t
ORIGIN      
        1 gtttatcacc accgactatt tgcaacagtg ccctggaaag tcagggcgaa tatgactcac
       61 aatgggcggc aatttgttcc attgctccaa agactggctg tacgccggag actctgcgtg
      121 tctgggttcg ccagtatgag cgggataccg ggggcggaga tggagggctc accagtgctg
      181 aacgtcagcg tctgaaagag ctggaacgtg aaaatcgtga actgcgccgc agcaacaata
      241 tccttcgcca ggcttccgct tattttgcga aggcggagtt cgaccgcctc tggaaaaagt
      301 gatggcactg ctggataagc tgcgtgagca gtacggggtc agaccggtat gcagtgaact
      361 gcatattgcc ccgtcaacgt attaccactg tcagcaacag cgacatcatc ctgataaacg
      421 cagtgcacat gcacagcgcg atgactggct gaagagagag atacagcgcg tatacgatga
      481 aaatcatcag gtgtacggtg tgcgtaaagt ctggcgtcag ttgttacggg aaggaatcag
      541 ggtggccaga tgtacagtgg cgcgcctcat ggcggttatg ggacttgccg gtgttctccg
      601 gggtaaaaag gtccgcacta ccgtcagccg gaaaaccgtt gccgcaggtg accgcgtaaa
      661 ccgtcagttc gtggcagaac gtcctgacca gctgtgggtg gctgatttta cttacgtcag
      721 cacatggcag ggcttcgtct atagtggcgt tcatcattga tgtatttgcc ggatatatcg
      781 tgggatggtg ggtatcatcg tccatggaaa cgacattcgt gctggatgca ctggagcagg
      841 cgttgtgggc ccgtcgtccg tc
//
LOCUS       DAMZFT010000359          854 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.363, whole genome shotgun sequence.
ACCESSION   DAMZFT010000359 DAMZFT010000000
VERSION     DAMZFT010000359.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 854)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 854)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..854
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.363"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..>854
                     /gene="dgoD"
                     /locus_tag="PN977_005402"
     CDS             <1..>854
                     /gene="dgoD"
                     /locus_tag="PN977_005402"
                     /EC_number="4.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020077623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="galactonate dehydratase"
                     /protein_id="HDI6045285.1"
                     /translation="AAVHELGDYLIGQDPSRINDLWQVMYRAGFYRGGPILMSAIAGI
                     DQALWDIKGKVLNAPVWQLMGGLVRDKIKAYSWVGGDRPADVIDGIKTLREIGFDTFK
                     LNGCEELGLIDNSRAVDAAVNTVAQIREAFGNQIEFGLDFHGRVSAPMAKVLIKELEP
                     YRPLFIEEPVLAEQAEYYPKLAAQTHIPLAAGERMFSRFDFKRVLEAGGISILQPDLS
                     HAGGITECYKIAGMAEAYDVTLAPHCPLGPIALAACLHIDFVSYNAVLQEQSMGIHYN
                     KGAELLDF"
BASE COUNT          197 a          212 c          244 g          201 t
ORIGIN      
        1 aagcggcagt tcacgagctg ggtgactatt tgattggtca ggatccatcg cgcatcaatg
       61 acttatggca agtgatgtat cgcgccggat tttatcgtgg cggtccaatc ctgatgagcg
      121 ccatcgccgg tatcgaccag gcgttatggg atatcaaagg caaagtgctg aatgcgccgg
      181 tctggcaact gatgggcggc ctggttcgcg acaaaattaa agcctacagt tgggtcggcg
      241 gcgatcgtcc ggcggatgtt atcgacggca ttaaaacgct acgcgaaatc ggcttcgata
      301 ccttcaaact gaacggttgt gaagaactgg ggctaattga taactcccgc gcggtagatg
      361 cggcagtcaa caccgtggca caaattcgtg aagcctttgg caatcagatt gagtttggtc
      421 ttgatttcca tggtcgcgtc agcgcgccga tggcgaaagt gctgattaaa gagctggaac
      481 cgtatcgtcc gctgtttatt gaagagccgg tgctggcgga acaggccgaa tactacccga
      541 aactggcggc acaaacgcat attccactgg cggcgggtga gcgcatgttc tcacgcttcg
      601 attttaaacg tgtgctggag gcaggcggta tttcgattct gcaaccggat ctctcccacg
      661 caggcggtat taccgaatgc tacaaaattg ccggaatggc agaagcctat gacgtgaccc
      721 ttgcgccgca ctgtccgctc ggaccgattg cactggcggc ttgcctgcat atcgactttg
      781 tttcctataa cgccgtcctt caggaacaaa gtatgggcat tcattacaac aaaggcgcgg
      841 agttactcga cttt
//
LOCUS       DAMZFT010000360          837 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.366, whole genome shotgun sequence.
ACCESSION   DAMZFT010000360 DAMZFT010000000
VERSION     DAMZFT010000360.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 837)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 837)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..837
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.366"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
BASE COUNT          214 a          184 c          238 g          201 t
ORIGIN      
        1 tgcgaaaaga tgcttccgca cgcacgcgcg ataaagaggg gatagttttc gtgaggacag
       61 ggagagaagc aggcgttctg ataattgcag agttgttttc ggaggtggtt acagtgagtc
      121 agcccgttcc tggcgagcag gagactgata tcaccgatgg gataaggttg tctgattggt
      181 attataaata taagaagtca tcagagagta acgtaaagga tttgccagaa taacctaaga
      241 gagtaaatct ctccggtaac agagcgctgt gaagcattct gtatcagagt gcagatacat
      301 gaacgctgat gcgtaaaatc agcagtgaca cgccagctcc ccctatgccc attcctgtat
      361 aagacatgaa ctgagtgtat taatgcttct gccctgagac tgagccggtg agtgaggggc
      421 tgagaaatcc ctcttgacac agctctaacc agaaatactt ccccggttgt tgcggtgcgt
      481 ccggatcccc gataacggaa cactaaggct gtattgcggg acaccgggta agtagcagcc
      541 cgtaaggcgt tctgtcaacg ccagaaaaaa tgatttccca cacgcgttat agactcactc
      601 ctgtggacct ggaagcccgt aactctgcgg ttgcccactg actgacggaa ctgaccgcaa
      661 attttacgag tgcgtgctgg gcttcagtct ggtcacctga gcgttcggta acggaggcgc
      721 gggcggtaat aggctgattt tcaggtatta ccggcttaca gcgtgtggtt gatattgagg
      781 ggctgtgagt ttcgcgtatt tgtcagaaac ccagacgagt tttcctgtat gggacga
//
LOCUS       DAMZFT010000361          831 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.367, whole genome shotgun sequence.
ACCESSION   DAMZFT010000361 DAMZFT010000000
VERSION     DAMZFT010000361.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 831)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 831)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..831
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.367"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            107..>831
                     /locus_tag="PN977_005403"
     CDS             107..>831
                     /locus_tag="PN977_005403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000486721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDI6045286.1"
                     /translation="MFRKIKIRKMTLNRALDKYLKTVSIHKKGHLQEFYRVNVIKRHP
                     IAERYMDDITTVDIANYRDQRLAQINPRTGRQITGNTVRLELALLSSLFNIARVEWGT
                     CRMNPVELVRKPKISSGRDRRLTSGEERRLSRYFKEKNQVLYVIFHLALETAMRQGEI
                     LSLRWEHVDLQHGVAHLPTTKNGAPRDVPLSRKARSYLQMLPTQLNGNIFSYTSSGFK
                     SAWRTALHELKIENLHFHDLRHEA"
BASE COUNT          237 a          203 c          176 g          215 t
ORIGIN      
        1 aatgttcctt ttctttgtta cccttccacg aaccggattg gcacagagac attgcatttc
       61 tgattcagta ttcctacctt cctcggattt atcgtggaag gcagatatgt ttcgaaaaat
      121 aaaaattcgc aaaatgactc tcaaccgggc gctggacaaa tacctgaaga cagtatccat
      181 ccacaaaaaa ggtcatctgc aggagtttta tcgcgtcaat gtgattaaac gtcaccctat
      241 tgctgaacgc tatatggatg acattaccac tgttgatatt gccaattatc gggatcaacg
      301 gttggcgcaa attaaccccc gaacggggcg tcaaatcacc ggaaacaccg tccgtcttga
      361 acttgccctg ttgtcatctt tattcaatat cgcacgagtg gaatggggaa catgccgcat
      421 gaatcctgtc gaactggtca gaaaacccaa aatcagtagc gggcgcgatc gtcgcctgac
      481 ttcaggagaa gaacgccgac tttcgcggta tttcaaagaa aaaaaccagg ttctttacgt
      541 catttttcat ctggcactgg aaacagcaat gcgacaaggt gaaatcttgt cattgcgttg
      601 ggaacacgtc gatctgcaac atggagtagc tcatcttcca acaaccaaaa acggtgctcc
      661 acgcgatgtt ccgctatcca ggaaagcccg tagttacctg caaatgctgc cgacacagct
      721 taacggtaat attttttctt atacctcttc gggattcaaa agtgcctggc gaacagcact
      781 tcacgaactg aagatagaaa atttgcattt tcatgacctc cgtcatgagg c
//
LOCUS       DAMZFT010000362          823 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.368, whole genome shotgun sequence.
ACCESSION   DAMZFT010000362 DAMZFT010000000
VERSION     DAMZFT010000362.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 823)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 823)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..823
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.368"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(15..>823)
                     /gene="iucB"
                     /locus_tag="PN977_005404"
     CDS             complement(15..>823)
                     /gene="iucB"
                     /locus_tag="PN977_005404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="N(6)-hydroxylysine O-acetyltransferase"
                     /protein_id="HDI6045287.1"
                     /translation="ALDQIFIAAPQLSAVVLPWSEWCEEPQALTLFGQVQSDIIHRSA
                     FWQLPLWLSSPANRASGEMVFDAEREIYFPQRPPRPQGEVYRRYDPRIRRMLSFRIAD
                     PVSDAERFTRWMNDPRVEYFWEQSGSLEVQIAYLERQLTSKHAFPLIGCFDDRPFSYF
                     EIYWAAEDRIGRHYSWQPFDRGLHLLVGEQQWRGAHYVQSWLRGVTHYLLLNEPRTQR
                     TVLEPRTDNQRLFRHLEPAGYRTIKEFDFPHKRSRMVMADRHHFFTEVGL"
BASE COUNT          199 a          224 c          228 g          172 t
ORIGIN      
        1 gtctttgtga ttcattacag accgacctcc gtgaagaagt gatgacgatc cgccatcacc
       61 atgcgcgagc gtttatgtgg gaagtcgaac tctttaattg tccggtatcc cgcaggctca
      121 agatggcgga acaggcgctg gttatcggtg cgtggctcca gtactgtgcg ctgcgtacgt
      181 ggctcattca gcagcaggta atgtgtcacc ccgcgcagcc agctttgcac atagtgggct
      241 ccgcgccatt gctgttcacc aaccagcaga tgcaggccac ggtcaaaggg ctgccacgaa
      301 tagtggcgac caatgcggtc ttccgccgcc cagtagattt cgaaataact gaacggccga
      361 tcgtcgaaac aaccaatcag cgggaatgca tgtttactgg tgagctggcg ttccagatag
      421 gcgatctgta cctccagtga gccactttgc tcccagaaat actcaacgcg cggatcgttc
      481 atccagcgag tgaaacgttc tgcatcagaa acgggatcgg caatgcggaa actcagcatc
      541 ctgcgaatgc gtggatcgta acgacgataa acttcaccct gcggacgggg ggggcgctgc
      601 gggaaataaa tctcacgctc tgcatcaaaa accatttcac cggaggcccg gtttgccgga
      661 gaactcagcc ataacggtaa ctgccagaaa gcggaacgat ggataatgtc gctttgtacc
      721 tgtccgaaaa gcgtcagcgc ctgtggctcc tcacaccatt cggaccaggg aagaaccact
      781 gctgaaagtt gtggtgcggc gataaatatc tgatccagcg cgt
//
LOCUS       DAMZFT010000363          782 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.369, whole genome shotgun sequence.
ACCESSION   DAMZFT010000363 DAMZFT010000000
VERSION     DAMZFT010000363.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 782)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 782)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..782
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.369"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..512
                     /gene="fliG"
                     /locus_tag="PN977_005405"
     CDS             <1..512
                     /gene="fliG"
                     /locus_tag="PN977_005405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HDI6045288.1"
                     /translation="TFGGVQPAALAELTEVLNGLLDGQNLKRSKMGGVRTAAEIINLM
                     KTQQEEAVITAVREFDGELAQKIIDEMFLFENLVDVDDRSIQRLLQEVDSESLLIALK
                     GAEQPLREKFLRNMSQRAADILRDDLANRGPVRLSQVENEQKAILLIVRRLAETGEMV
                     IGSGEDTYV"
     gene            505..>782
                     /gene="fliH"
                     /locus_tag="PN977_005406"
     CDS             505..>782
                     /gene="fliH"
                     /locus_tag="PN977_005406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416450.2"
                     /note="binds to and inhibits the function of flagella
                     specific ATPase FliI; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliH"
                     /protein_id="HDI6045289.1"
                     /translation="MSDNLPWKTWTPDDLAPPQAEFVPMVEPEETIIEEAEPSLEQQL
                     AQLQMQAHEQGYQAGIAEGRQQGHEQGYQEGLARGLEQGLAEAKSQQAP"
BASE COUNT          192 a          204 c          247 g          139 t
ORIGIN      
        1 ccacctttgg cggcgtgcag ccagccgcgc tggcggaact gaccgaagta ctgaatggct
       61 tgctcgacgg tcagaatctc aagcgcagca aaatgggcgg cgtgagaacg gcagccgaaa
      121 ttatcaacct gatgaaaact cagcaggaag aagccgttat taccgccgtg cgcgaattcg
      181 acggcgagct ggcgcaaaaa attatcgacg agatgttcct gttcgaaaat ctggtggatg
      241 tcgacgatcg cagcattcag cgtctgttgc aggaagtgga ttccgaatcg ctgttgatcg
      301 cgctgaaagg ggccgagcag ccactgcgcg agaaattcct gcgcaatatg tcgcagcgtg
      361 ccgccgatat tctgcgcgac gatctcgcca accgtggtcc ggtgcgtctg tcgcaggtgg
      421 aaaacgaaca gaaagcgatt ctgctgattg tgcgccgcct tgccgaaact ggcgagatgg
      481 taattggcag cggcgaggat acctatgtct gataatctgc cgtggaaaac ctggacgccg
      541 gacgatctcg cgccaccaca ggcagagttt gtgcccatgg tcgagccgga agaaaccatc
      601 attgaagagg ccgaacccag ccttgagcag caactggcgc aactgcaaat gcaggcccat
      661 gagcaaggtt atcaggcggg gattgccgaa ggtcgccagc aaggtcatga gcagggctat
      721 caggaaggac tggcccgggg gctggagcaa ggtctggcag aggcgaagtc tcaacaagcg
      781 cc
//
LOCUS       DAMZFT010000364          760 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.370, whole genome shotgun sequence.
ACCESSION   DAMZFT010000364 DAMZFT010000000
VERSION     DAMZFT010000364.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 760)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 760)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..760
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.370"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..600
                     /locus_tag="PN977_005407"
     CDS             <1..600
                     /locus_tag="PN977_005407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1281 domain-containing protein"
                     /protein_id="HDI6045290.1"
                     /translation="GTGQRRWEGLPDEVRETITVHFTAKRGDWCGFWSNEDVSVWWNR
                     LCDNVLPEKTMPFDLLTVLPTRLDVEVNGFNGGVLNGVPSAYHWYTEQYGVKWPVGYE
                     VNISRQGENFIQVDFDTPWCQPESNVVAELSRRFGCTLEHWYAEQGCNFCGWQRYERG
                     ELVDVLWGELEWSSPTDDDELPEVTAPEWIVDKVAHYGG"
BASE COUNT          153 a          176 c          258 g          173 t
ORIGIN      
        1 ggtactggcc agcgtcgctg ggaaggatta ccggatgagg tcagggaaac catcaccgtg
       61 catttcaccg caaaaagagg tgactggtgt ggcttctgga gtaacgagga tgtatcggtg
      121 tggtggaacc gtctgtgtga caatgtactg ccggaaaaaa ccatgccgtt tgacctgctg
      181 acggttctgc ccacccgcct ggatgttgaa gtgaatggct ttaacggtgg tgttctgaac
      241 ggtgttcctt ctgcgtatca ctggtatacg gaacagtatg gcgtgaagtg gcctgtgggg
      301 tatgaggtga atatcagccg tcagggagaa aacttcattc aagtggattt cgacacgccg
      361 tggtgtcagc cggaaagcaa cgttgttgca gaattaagcc gccgtttcgg ctgcacgctg
      421 gagcactggt acgccgaaca gggctgcaat ttctgtggct ggcagcgata tgagcgtgga
      481 gagcttgttg atgtgctgtg gggggaactg gaatggtctt ccccgacaga tgatgatgag
      541 ctgccggaag tcaccgcgcc ggagtggata gtcgacaagg tggcgcatta cgggggatga
      601 agtatgacgg agacgggcgg gcaaccgccc gtttcttttc cgacaaagga tgtcgccgtg
      661 ctcctctttt tactgcgccg gctgacgcgg cgcggcagga acgctgcctg tggtcagtgt
      721 cccgcgtccg gcgggcacgg gacgggcgct tttactgctc
//
LOCUS       DAMZFT010000365          742 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.371, whole genome shotgun sequence.
ACCESSION   DAMZFT010000365 DAMZFT010000000
VERSION     DAMZFT010000365.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 742)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 742)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..742
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.371"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(46..591)
                     /locus_tag="PN977_005408"
     CDS             complement(46..591)
                     /locus_tag="PN977_005408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000744644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6045291.1"
                     /translation="MKKQPLLWMTIAAALLSGCTGHREHHPQVTFTVDTVRTDTTASN
                     PICLAEKYGSLLNERHPDLFADAQGVISHSAFFNDSLTLTPETLHGMMQNTSCVESQV
                     TSVGPDAFTADVSPDVRMQISPDIKGGNITLRLSTTDQKRNQTVEQSISLRPEQSLVM
                     NGFAGGGASEKRVVLITPHVR"
BASE COUNT          167 a          180 c          204 g          191 t
ORIGIN      
        1 agggcggtac agtctgcgaa gactggacac ggggtgtgtc gggaattaac gcacgtgagg
       61 cgtaatgaga acgacgcgtt tttcgctagc tccacctccg gcgaatccat tcatgacaag
      121 ggactgttcc ggtctgagag aaatgctctg ctcaacagtc tggtttcgtt tctggtctgt
      181 ggtcgagagt cgcagagtga tattgccccc cttaatatcc ggcgatatct gcattctgac
      241 atcgggggat acatcagcag taaaggcatc cggacctacg gatgttacct gtgattcaac
      301 acaggacgta ttctgcatca tcccgtggag cgtttcaggt gtcagcgtca ggctgtcatt
      361 aaaaaaagca ctgtgtgaaa tgaccccctg agcatcggca aacaggtccg gatggcgctc
      421 attaagaagg cttccgtatt tttccgccag gcagattggg ttgctggccg ttgtgtctgt
      481 tcgcacggta tcgaccgtga atgtcacctg cgggtgatgt tccctgtggc ctgtgcagcc
      541 ggacagcagt gcggcggcta tcgtcatcca gagtagtggt tgttttttca tgctgttaat
      601 ttcctctcag ggtaaaaaga aagactcgca ccgtcatgtc aatggtaagc gtacagcctg
      661 aaccgtctgg tcagaatctg acgaattaga cataggggca aatccatgtt tattttgcat
      721 ccatcatctc gcccatcagc ag
//
LOCUS       DAMZFT010000366          729 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.372, whole genome shotgun sequence.
ACCESSION   DAMZFT010000366 DAMZFT010000000
VERSION     DAMZFT010000366.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 729)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 729)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..729
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.372"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..332
                     /gene="sitA"
                     /locus_tag="PN977_005409"
     CDS             <1..332
                     /gene="sitA"
                     /locus_tag="PN977_005409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000949004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="iron/manganese ABC transporter substrate-binding
                     protein SitA"
                     /protein_id="HDI6045292.1"
                     /translation="GAFSYLARDLGLKELYLWPINADQQGTPQQVRKVVDIVKKNHIP
                     AVFSESTISDKPARQVARETGAHYGGVLYVDSLSTENGPVPTYIDLLKVTTSTLVQGI
                     KAGKREK"
     gene            332..>729
                     /gene="sitB"
                     /locus_tag="PN977_005410"
     CDS             332..>729
                     /gene="sitB"
                     /locus_tag="PN977_005410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter ATP-binding
                     protein SitB"
                     /protein_id="HDI6045293.1"
                     /translation="MMQSAGIVVNDVTVTWRNGHTALRDASFTVPGGSIAALVGVNGS
                     GKSTLFKAIMGFVRLTSGKISVLGIPTRQALQKNLVAYVPQSEEVDWSFPVLVEDVVM
                     MGRYGHMGMLRIAKKRDRQIVTDALERVDMV"
BASE COUNT          184 a          158 c          206 g          181 t
ORIGIN      
        1 aaggggcttt ttcttatctc gcacgcgatt tggggctaaa agagctttat ctgtggccga
       61 ttaatgccga tcaacaagga acaccgcagc aggtacgtaa ggttgttgat atagttaaga
      121 aaaatcatat cccggcagtc tttagcgaga gtacgatttc cgataaacca gcgcgtcagg
      181 ttgcgcgtga aaccggcgcg cactacggtg gtgtgctcta tgtcgattcc ctgagcacag
      241 aaaacggccc ggtacctacg tatatcgacc ttctgaaggt tactaccagt acgctggtac
      301 agggaattaa agccggaaaa agggagaaat aatgatgcaa tctgcaggca ttgtcgttaa
      361 tgatgttact gtcacctggc gtaacgggca cacagcactg cgcgatgcat ccttcacagt
      421 accaggcgga tctattgccg ctctggttgg ggtaaacggt tccggaaaat cgacgttgtt
      481 taaagcgatt atgggatttg tgcgtctgac cagcgggaag atatctgttc tgggtattcc
      541 cacacgacag gcgctacaga aaaacctggt tgcctacgtc cctcagtcag aagaggttga
      601 ctggtcattt cctgtactgg tggaagatgt ggtgatgatg ggccgctatg ggcatatggg
      661 tatgttgcgt atcgccaaaa agagggatcg tcagattgtc accgatgcgc tggaacgtgt
      721 cgatatggt
//
LOCUS       DAMZFT010000367          713 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.373, whole genome shotgun sequence.
ACCESSION   DAMZFT010000367 DAMZFT010000000
VERSION     DAMZFT010000367.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 713)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 713)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..713
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.373"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(<1..678)
                     /locus_tag="PN977_005411"
     CDS             complement(<1..678)
                     /locus_tag="PN977_005411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001545778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HDI6045294.1"
                     /translation="MASAEVARDFVELHLPAEYRQLCDLSTLRLEPCTFVDQDLKQYA
                     CDALFSMKTISGEDGYIYCLAEHQSTENAFMAFRMLRYAVAAMQRHFEQYHELPLVIP
                     LLFYHGERSPYPYSMNWLECFRNPELAAKIYTRPFHLVDVTVLDDDEIMQHRRMGALT
                     LLMKHSRSRDIMLQMDRLVQLIQTSLNEELAKALFHYLLNGSEHVTVRFLQTLAERLP
                     QHEGNIMT"
BASE COUNT          156 a          217 c          183 g          157 t
ORIGIN      
        1 tgtcattatg ttgccctcgt gctgtggcag ccgctccgcc agcgtctgca aaaaccttac
       61 cgtcacatgt tcactgccgt tcagcagata gtggaacaga gccttcgcca gctcttcatt
      121 cagtgaagtc tgtatcagct gaaccagcct gtccatctgc agcatgatgt cgcggctgcg
      181 gctgtgtttc atcagcagcg tcagtgcccc catacgacgg tgctgcatta tctcatcgtc
      241 atccagtacc gtcacgtcca ccaggtggaa cggtctggtg tagattttag cagcaagttc
      301 cggatttctg aaacactcca gccagttcat gctgtacggg tacggactcc gctcgccgtg
      361 gtagaacagc agcgggatca ccagcggcag ctcatggtac tgttcaaagt gacgctgcat
      421 cgccgccacc gcataccgga gcatcctgaa cgccataaac gcgttttccg tgctctggtg
      481 ttccgcgagg caataaatat acccgtcttc accgctgatg gtcttcatgc tgaacagcgc
      541 atcacacgca tactgtttca gatcctgatc cacaaaggta cagggctcca gccgcagcgt
      601 cgacagatca cacaactgcc ggtattccgc cggaagatgc agctcaacaa aatcacgggc
      661 cacctctgcg gaggccataa acgccctgaa ggcggcatca tgcggcgtgg ggg
//
LOCUS       DAMZFT010000368          704 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.374, whole genome shotgun sequence.
ACCESSION   DAMZFT010000368 DAMZFT010000000
VERSION     DAMZFT010000368.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 704)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 704)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..704
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.374"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            61..486
                     /locus_tag="PN977_005412"
                     /pseudo
     CDS             61..486
                     /locus_tag="PN977_005412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000207962.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
BASE COUNT          200 a          144 c          182 g          178 t
ORIGIN      
        1 attaagcgca atataacggt tatggatcgt tatcctgatt tagtaactgc acaaatggat
       61 ctggaagtat acaaaatcgc gctggcatca ctggaagcag aaccagttgc ttatattttc
      121 aaacatccgg ccgggaaatt attctgggct ttaacggatg aaagcaataa agagcaaccg
      181 gacgttattc ctgtttatgc cgcccctcca gtgccagtag taccggaaga aaaaccaatg
      241 cctaacgctc taagcatgct tgaaagagag atcacacaac tgattggtga tgcgcaggaa
      301 gccactgtta ctggctatga gttaatcgct gaagcctggc gtttgatgga tggacaagac
      361 cctaaaacca gcgattggca tagcaaggct tcgaagtatt taaattccaa tattgtagaa
      421 aaagttgatg atgaccgtat tgaggctgtt aaggctgttt tgcgtagact ggctggcaac
      481 tctccggtaa ttccaggtga ggtgctgtca gcaattatga aggttgccag ggttcgtgcc
      541 gatttcgatg attttgacgg tgacaggcga ggtatcggtg attgtctgga tgaggctgag
      601 caagagctta tcgttaccat taacaaatat gccagtcagt tggcagcaga accgatagcg
      661 actaatgacg ttcgagagca gcagacagcc gttccgccag ttcc
//
LOCUS       DAMZFT010000369          671 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.375, whole genome shotgun sequence.
ACCESSION   DAMZFT010000369 DAMZFT010000000
VERSION     DAMZFT010000369.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 671)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 671)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..671
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.375"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            1..116
                     /locus_tag="PN977_005413"
                     /pseudo
     CDS             1..116
                     /locus_tag="PN977_005413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001119429.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            171..431
                     /locus_tag="PN977_005414"
     CDS             171..431
                     /locus_tag="PN977_005414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001370055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDI6045295.1"
                     /translation="MELADYCRRTGLFVEQVKEWRAISIQAHEKKLTENIRTDRALRD
                     AKNKLRELEKELKRKDKALAEAAALLILREKFNALWDNSEED"
     gene            383..>671
                     /locus_tag="PN977_005415"
     CDS             383..>671
                     /locus_tag="PN977_005415"
                     /inference="COORDINATES: protein motif:HMM:NF024776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDI6045296.1"
                     /translation="MKGKVQCPLGQQRGRLTPLLERRKIISLIHEAVINGARKSQACR
                     CIGISVRTLQRWTNGKVITTDRRSDTVNRSPRNKLSEAERLRILELCNSPEF"
BASE COUNT          200 a          138 c          178 g          155 t
ORIGIN      
        1 agatagcccg taagctgaag atcagtgcct cagttgtatc aaaatggaga tgtgagttag
       61 tggagcaagg acttttacct gaaaatgaaa agattacctc cgttgatgtt gaagactgga
      121 caccggagcg tcgattctca gttgttcttg agacggctac gctgtcagaa atggaacttg
      181 ctgattattg ccgacgcacg ggattgtttg ttgaacaggt taaagaatgg cgggctatct
      241 caattcaggc gcatgaaaag aaattgacgg aaaatatccg aacagacagg gcattgcgtg
      301 acgcaaaaaa taagttacga gaactggaga aagagcttaa gcgcaaagat aaagctctgg
      361 cggaagcagc tgccctgctt atattaaggg aaaagttcaa tgccctctgg gacaacagcg
      421 aggaagactg acccctctcc tggagcggcg taaaattatt tctcttatcc acgaggccgt
      481 tatcaacggc gcccggaaaa gtcaggcttg taggtgtatc ggtatttctg ttcggacact
      541 tcagcgctgg actaatggca aagtcatcac cacggaccga agatccgata ctgtaaacag
      601 atctccccga aataagctgt cagaagcgga aagattgcgg atactggagt tgtgcaactc
      661 tccagagttt g
//
LOCUS       DAMZFT010000370          665 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.376, whole genome shotgun sequence.
ACCESSION   DAMZFT010000370 DAMZFT010000000
VERSION     DAMZFT010000370.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 665)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 665)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..665
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.376"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            100..510
                     /locus_tag="PN977_005416"
     CDS             100..510
                     /locus_tag="PN977_005416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001510726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type cleavage/methylation
                     domain-containing protein"
                     /protein_id="HDI6045297.1"
                     /translation="MSQMSSTMSSSDNQREVKNLQTIATKMKQYKFQGQYTSSDYIKT
                     LTQSGSLPADMIAGGNKAKNAWGGDVTIKATADKYGYTITSNNVPKENCVELINSLRS
                     SSMFTKIMNTAPATVDPVTVCSNDKNNITLETNS"
BASE COUNT          209 a          145 c          142 g          169 t
ORIGIN      
        1 tattttgctt taaaaaaaga gaaagaggag gataaaggtt taaccttact ggaagttgcc
       61 ggcgcactga ttattatcgg tattgtggtt gttctcgcaa tgagtcagat gtccagtacc
      121 atgtcatcca gtgataacca gcgcgaagtg aaaaacctgc agaccattgc aaccaaaatg
      181 aagcagtaca agttccaggg acaatatacc agcagtgatt atatcaaaac gctgacgcag
      241 agtggttccc tgccggccga tatgatcgcc ggtggtaaca aggccaaaaa tgcctggggc
      301 ggtgatgtca ccattaaggc aacggcagac aagtatggtt atacgattac cagcaataat
      361 gtaccgaaag aaaattgcgt ggaactcatt aacagcctgc gcagttcatc catgtttacc
      421 aaaatcatga ataccgcccc ggctactgtg gatcccgtaa cagtatgtag taacgacaaa
      481 aataacatca cccttgaaac aaacagctaa tgtttccgga cagcatcctt aaataccaga
      541 ggatgctgtc actgccggga ttatcataat gaaatggtta tatctctctg tcctgctttt
      601 attcagtcac acatcaatcg catctgtgat gaataataat catctcgatt cctgttttat
      661 tcagg
//
LOCUS       DAMZFT010000371          655 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.377, whole genome shotgun sequence.
ACCESSION   DAMZFT010000371 DAMZFT010000000
VERSION     DAMZFT010000371.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 655)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 655)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..655
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.377"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..>655
                     /locus_tag="PN977_005417"
     CDS             <1..>655
                     /locus_tag="PN977_005417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000842452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HDI6045298.1"
                     /translation="WSAFAEDLNADATVMTTAHHTDTTETASPSEPAGTQPVQQATPE
                     STAENKPQEEDVLSATDTSEKSASATTADNQSVQAEELSPGDDTPETSLSDAAGTQHT
                     TTTQSEEQVQVSMASADDWSAFAEDLNADTPGAPTVHNTDNAETTTVSEPAGMRPVQQ
                     VTTELTAEDKAQEKSVASAAETGAEHSSDITDDNRNAPAEELNADATVAATVHNTGTA
                     "
BASE COUNT          181 a          212 c          170 g           92 t
ORIGIN      
        1 tggagcgcat ttgcggaaga cctcaacgct gatgccactg tgatgaccac ggctcaccac
       61 accgatacaa cagaaacagc ctcaccttct gaacctgccg ggactcagcc tgttcagcag
      121 gcaacccctg aatcaacggc agaaaataaa ccgcaggaag aggatgttct gtctgcaacg
      181 gataccagtg aaaagagtgc ctctgccacc accgctgaca accagagcgt acaggcagaa
      241 gagctctccc caggtgacga cacaccggaa acctccctct ccgacgcggc cgggacacag
      301 cacaccacca caacgcagtc tgaagaacaa gtgcaggtct ccatggccag cgctgacgac
      361 tggagcgcat ttgcggaaga tctcaacgct gacactccgg gggcccccac tgttcacaac
      421 accgacaacg cggagacaac cacagtttct gaaccggccg ggatgcggcc agttcagcag
      481 gtgaccactg aactgacggc agaagataaa gcacaggaaa agagcgttgc gtctgccgct
      541 gagaccggcg cagagcactc ctctgacatc accgatgaca acaggaacgc acccgctgag
      601 gagctcaatg ccgatgccac ggttgctgcc actgttcaca acaccggcac agcag
//
LOCUS       DAMZFT010000372          650 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.378, whole genome shotgun sequence.
ACCESSION   DAMZFT010000372 DAMZFT010000000
VERSION     DAMZFT010000372.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 650)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 650)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..650
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.378"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(<1..>650)
                     /locus_tag="PN977_005418"
     CDS             complement(<1..>650)
                     /locus_tag="PN977_005418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000670135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HDI6045299.1"
                     /translation="NRVEFYEALGGMIRDGTPSLRALEFLCEIETDFGEKKGTSGMYF
                     LAKECIDSIKASGMLSGALLTWVPPEEATLIRNGEERGNIADALFQVARTVKARREMT
                     SSLIAVCLYPLLLLSLCVVNMYNAHTRIIPIVSAFASEDKWSFQMKLLAGMSEFFIGY
                     GFWLCGTIFALTLLIRFSFSRYTGPGRRMLDKAPPWSLYQTLHGVNYLFSISSMLQ"
BASE COUNT          163 a          167 c          178 g          142 t
ORIGIN      
        1 atctgcagca tggatgaaat actgaacaga taattcaccc cgtgtaaggt ctgatacagt
       61 gaccaggggg gagccttgtc cagcatcctc cggccggggc cggtataccg gctgaatgaa
      121 aaacggatca gaagcgtcag ggcgaaaatg gtcccacaga gccagaatcc gtacccgatg
      181 aaaaattccg acatgccggc aagcaatttc atctggaatg accatttatc ttctgaagca
      241 aacgcactga cgatggggat gatacgggta tgggcgttgt acatattcac cacacagagc
      301 gaaagcagca gaagggggta gaggcagacg gcaatgaggc tggatgtcat ttcccgccgg
      361 gctttaacgg ttctggccac ctggaaaagg gcgtcggcaa tatttccgcg ctcttccccg
      421 tttctgatta acgtggcttc ctccggtggc acccaggtta acagggcacc ggataacatg
      481 ccgctggctt tgatggaatc gatgcattct tttgccagaa aatacatgcc ggatgtgcct
      541 ttcttttcac caaaatcggt ttcgatctcg cagagaaatt cgagtgcacg cagggacggc
      601 gttccgtcac gtatcatgcc gcccagggct tcgtagaact ccacccggtt
//
LOCUS       DAMZFT010000373          645 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.379, whole genome shotgun sequence.
ACCESSION   DAMZFT010000373 DAMZFT010000000
VERSION     DAMZFT010000373.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 645)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 645)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..645
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.379"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(<1..476)
                     /locus_tag="PN977_005419"
     CDS             complement(<1..476)
                     /locus_tag="PN977_005419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000919993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunoglobulin-binding protein"
                     /protein_id="HDI6045300.1"
                     /translation="MLKRRDAFLKKSALAVSVALLLSAQAQAQDILIGPIQPGEDNSF
                     LVGESVAGRSIDKVRNVWLIGDDSFLLDSNRTVLLGNNSGVVNSPGSVSLGHDALIAD
                     SEWGTVAGKEASLISSRQSSAIGAFSSVQDSTSSVALGHGSQVSGENNVVSVGAGP"
BASE COUNT          203 a          167 c          114 g          161 t
ORIGIN      
        1 tcaggcccgg caccaacaga taccacgtta ttttctcccg acacctgtga gccatggccc
       61 agagctacag aactggtaga atcctgcaca gatgaaaacg caccaattgc cgagctctga
      121 cgagaagaaa taagtgacgc ttcttttcct gcaacagtcc cccattcaga gtcagcaata
      181 agtgcatcat gccccagact cacacttccc ggagagttca caacaccaga attattcccc
      241 aaaagcacag tcctgtttga gtctaataaa aaagaatcat caccaatcaa ccatacattt
      301 cttactttat caatcgaacg gccagctact gattctccaa caagaaagct attatcttcg
      361 ccaggttgaa tcggtccgat taaaatatcc tgagcctgag cctgagccga gagcagcagg
      421 gccacactca ctgccagcgc gcttttcttt aaaaaagcat cacgtctttt aagcattctg
      481 ttattctcct gtattattac ttcaccagcc agaacggtag tgcggagcaa aaatacagtg
      541 ataagaatcc tcttttatta atttaataat gccaggaata ttattgtgta taatatgcgc
      601 cctcaattta agcagcagaa aacgactggg attaaaatcc ttaaa
//
LOCUS       DAMZFT010000374          638 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.380, whole genome shotgun sequence.
ACCESSION   DAMZFT010000374 DAMZFT010000000
VERSION     DAMZFT010000374.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 638)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 638)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..638
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.380"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            237..557
                     /locus_tag="PN977_005420"
     CDS             237..557
                     /locus_tag="PN977_005420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5375 domain-containing protein"
                     /protein_id="HDI6045301.1"
                     /translation="MKTPLPPVLRAALYRRAVACAWLTVCERQHRYPHLTLESLEAAI
                     AAELEGFYLRQHGEEKGRQIACALLEDLMESGPLKAAPSLSFLGLVVMDELCARHIKA
                     PVLH"
BASE COUNT          149 a          193 c          175 g          121 t
ORIGIN      
        1 tcagccgggc ggcaccggtg gcgcgggaat ggcgggaaat tccccgcacg gagcctgacc
       61 cggaaaccac cccggataac agttaccgca attatctgta attaaaaaac gacacccgaa
      121 aaattaaatg tgcgtattcg cgcagggata cgcatgtctt caggagacgc agatatgcct
      181 tatcagttaa tgcaaccggc acggaatgca gtcatctgtc acagggagga aagcaaatga
      241 aaacaccctt accgcccgtc ttacgcgctg ccctttaccg tcgcgctgtc gcctgtgcct
      301 ggctgaccgt gtgcgaacgt cagcaccgct acccgcatct cacccttgag tcactggagg
      361 cggccatcgc cgctgagctg gaaggctttt atctgcgcca gcacggtgag gaaaaagggc
      421 gtcagatagc ctgtgccctg ctggaagatt taatggaatc cggccccctg aaggccgcgc
      481 cgtcgctgtc ctttctcggg ctggttgtga tggatgaact ctgtgcccgt cacataaaag
      541 cgccggtact gcactgaagg agaacaacac catgaaaatg aacgtaaccg ccaccgtcag
      601 ccatgcgctc ggccactggc cgcgtatcct cccggcgc
//
LOCUS       DAMZFT010000375          609 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.381, whole genome shotgun sequence.
ACCESSION   DAMZFT010000375 DAMZFT010000000
VERSION     DAMZFT010000375.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 609)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 609)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..609
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.381"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..347
                     /locus_tag="PN977_005421"
     CDS             <1..347
                     /locus_tag="PN977_005421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="argininosuccinate synthetase"
                     /protein_id="HDI6045302.1"
                     /translation="GRWFDSQALMLRDSLQRWVASQITGEVTLELRRGNDYSILNTVS
                     ENLTYKPERLTMEKGDSVFSPDDRIGQLTMRNLDITDTREKLFGYAKTGLLSSSAASG
                     VPQVENLENKGQ"
     gene            complement(355..>609)
                     /locus_tag="PN977_005422"
     CDS             complement(355..>609)
                     /locus_tag="PN977_005422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417641.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PsiE"
                     /protein_id="HDI6045303.1"
                     /translation="PVLGDGVDRTTENDIFSTAYNNYLINAWMGVTKPEQPQTLEEVI
                     AHYKGDSRVVDANHDVFDFVMLRKEFTEDKQGNPTPEGQG"
BASE COUNT          141 a          169 c          147 g          152 t
ORIGIN      
        1 agggacgttg gtttgactcc caggcgctga tgctgcggga ctctctgcaa cgctgggttg
       61 ccagccagat cactggtgag gttaccctgg agctgcgccg tgggaacgat tattcaatcc
      121 tgaataccgt ctcagagaac ctgacctaca agccagagcg tctgacgatg gaaaaaggcg
      181 actcggtgtt ctcgccagat gatcgtatcg gtcaactgac catgcgcaac ctggatatca
      241 ccgatacccg cgagaaactt ttcggttatg ccaaaactgg cctcctctcc tcctctgccg
      301 cttcgggcgt gccgcaggtg gagaatctgg aaaataaagg ccagtaattc gacatcaacc
      361 ctgcccttca ggggtggggt taccttgctt atcctcagta aactcctttc ttagcatcac
      421 aaaatcgaaa acatcatggt ttgcatctac aacccgtgag tctcctttat agtgtgcaat
      481 cacttcctcc agcgtttgcg gctgttccgg ctttgttacc cccatccacg cattaataag
      541 gtaattattg tacgcagtgg aaaagatatc gttttccgtt gtgcgatcca cgccatcccc
      601 aagaacggg
//
LOCUS       DAMZFT010000376          606 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.382, whole genome shotgun sequence.
ACCESSION   DAMZFT010000376 DAMZFT010000000
VERSION     DAMZFT010000376.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 606)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 606)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..606
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.382"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            96..254
                     /locus_tag="PN977_005423"
     CDS             96..254
                     /locus_tag="PN977_005423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001351253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HDI6045304.1"
                     /translation="METLSTNLQLARLVGVQGTPATIIGDEMIPGAVSWETLEAVVKE
                     KLAVAHAQ"
     gene            244..>606
                     /locus_tag="PN977_005424"
     CDS             244..>606
                     /locus_tag="PN977_005424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003846856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin family protein"
                     /protein_id="HDI6045305.1"
                     /translation="MLSKLRRWLREGAILLVVLAGVIILLDVWRSPQMPAMFDSTPLH
                     TLDGETVTLASISEERPVLLYFWASWCGICRFTTPDVARLQSEGESVMTIALRSGNDG
                     EVSRWLSRKRVTFPVVNDS"
BASE COUNT          126 a          161 c          193 g          126 t
ORIGIN      
        1 gcagacagaa accgtaaaca ccggcattga catgccgggg aagcccttat agtacaataa
       61 ttctctatat ccaaactgac cccgatgaga aaagcatgga gacgttgagc accaatctgc
      121 aactcgcccg gctggttggc gttcagggga cgccggcaac catcattggc gacgagatga
      181 tccccggcgc ggtatcgtgg gagacccttg aggcggtggt gaaagaaaaa ctggcggtgg
      241 cccatgctca gtaaactgcg ccgctggctg cgtgaaggcg cgatcttact ggttgtgctg
      301 gctggcgtga taatcctgct ggatgtctgg cgttctccgc agatgccggc catgtttgac
      361 agcacgccgc tgcatacgct ggacggggaa accgtaacgc tggcgtcgat cagtgaagaa
      421 cgcccggtgc tgctctactt ctgggcgagc tggtgcggta tctgccgttt caccacgccg
      481 gatgtcgccc gtttgcagtc ggaaggggag agcgtcatga ccatcgcgct gcgttccggt
      541 aatgacgggg aggtttcccg ctggttatcg cgtaaaaggg tgacgttccc cgtcgtgaat
      601 gatagc
//
LOCUS       DAMZFT010000377          601 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.383, whole genome shotgun sequence.
ACCESSION   DAMZFT010000377 DAMZFT010000000
VERSION     DAMZFT010000377.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 601)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 601)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..601
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.383"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(<1..162)
                     /locus_tag="PN977_005425"
     CDS             complement(<1..162)
                     /locus_tag="PN977_005425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001314127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6045306.1"
                     /translation="MILPSEKSATDVAAQCFLNALIRETKDWQLAEYPPDELIIPLDE
                     QKSLHFRVAY"
     gene            298..>601
                     /locus_tag="PN977_005426"
     CDS             298..>601
                     /locus_tag="PN977_005426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDI6045307.1"
                     /translation="MSSKIEDTPQKTLSCWPLAFSAGLLGIGQNGLLVVLPVLVIQTN
                     LSLSVWAALLMLGSMLFLPSSPWWGKQISLTGSKTVVLWALGGYGVSFTLLGLGSVL"
BASE COUNT          157 a          133 c          144 g          167 t
ORIGIN      
        1 ataagccact ctgaaatgga gcgacttctg ttcgtccagc gggataatca attcgtctgg
       61 cgggtattcc gccagttgcc agtctttggt ttcacgaatc aaggcattca ggaagcactg
      121 cgcagccaca tctgtggcgg atttttcaga gggcaggatc ataatcaaac agctcctaca
      181 cagtaaaata ataacaatta tgtcaataat gattatcatt atcaatgaga aatgttattg
      241 tcagaaaaat taattttaca aataaacaca attaatacat ggtgttgaaa cattacaatg
      301 tcatctaaga tcgaagatac gccccaaaaa accctgtcct gctggccact ggcgttcagt
      361 gccggtcttc tcggtatcgg acagaacggt ctgctggttg tactccctgt tctggtcata
      421 cagacaaatc tgagtctgtc tgtatgggct gccctgctga tgctgggctc aatgctgttt
      481 ctgccatctt ccccatggtg gggaaagcaa atttccctta ctggcagtaa gactgtggtg
      541 ctgtgggctc tgggaggata tggcgtaagc tttaccctgc ttgggctggg aagcgtgctg
      601 a
//
LOCUS       DAMZFT010000378          598 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.384, whole genome shotgun sequence.
ACCESSION   DAMZFT010000378 DAMZFT010000000
VERSION     DAMZFT010000378.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 598)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 598)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..598
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.384"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            209..>598
                     /locus_tag="PN977_005427"
     CDS             209..>598
                     /locus_tag="PN977_005427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001609235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ead/Ea22-like family protein"
                     /protein_id="HDI6045308.1"
                     /translation="MSEIDYQALRERYSPKPVPECSVCGEEMSIQRISGAHVVYACSG
                     EGDDGYFKIGRTFADEHYEESRVTVVDDSDPDVIALLDELEAAKSKLNEQREYYEGVI
                     ADGSKRIAELEAREIKPAKGEVLVVVSG"
BASE COUNT          181 a          110 c          166 g          141 t
ORIGIN      
        1 atgagcaagc caaccgacga agaaatagtt cgggtgttgg aagaacacgg gcgctgtatg
       61 acttacgtcg tgaccaactg gcttagggat aaatatcgca cactcaaaac ggcatacgtt
      121 ttgcgtcgtc taaagaaact ggagttcgac gggaaagtaa agcgcgttaa cagttcttat
      181 ataagacaaa tttgctggga ggcaaccagt gagcgagatt gattatcagg cgctacggga
      241 acgttattca cctaagccag tacctgaatg ctctgtttgt ggcgaggaaa tgtcaataca
      301 gcgcatatct ggagcacatg ttgtttatgc ctgttccggt gagggtgatg acggatattt
      361 taagataggg cgtacttttg cagacgaaca ttatgaagaa tcacgcgtaa cagtagttga
      421 tgatagtgat cccgatgtga ttgcactact ggacgaactg gaggcagcaa aatcaaaact
      481 caacgagcag cgtgaatatt acgagggagt aatcgcggat gggagtaagc gtattgcaga
      541 actggaagca cgggaaataa aaccagccaa aggcgaagtt cttgtcgttg tatctggt
//
LOCUS       DAMZFT010000379          593 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.385, whole genome shotgun sequence.
ACCESSION   DAMZFT010000379 DAMZFT010000000
VERSION     DAMZFT010000379.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 593)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 593)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..593
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.385"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(<1..571)
                     /locus_tag="PN977_005428"
     CDS             complement(<1..571)
                     /locus_tag="PN977_005428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000980235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
                     /protein_id="HDI6045309.1"
                     /translation="MMMPVLQKVPFSGLPFSGICGYSFSAVAKSAAGRRNPCNSKATY
                     DAPCVFFCVAINATERQIMVWRVVCRAGMILFAIACYATESMVAQAGQPPGWPVFFEA
                     GIPTPVWAIAIERRNSGDSSYLLLEGDCLMATTLTPSHPEFVFVFAAVRRADRHPRIC
                     MLRTVAGDERSARRSLVRDYVLSLAARLPV"
BASE COUNT          158 a          162 c          174 g           99 t
ORIGIN      
        1 tcaccggcag acgggcagca agggagagca catagtcacg gacaagggaa cggcgggcgc
       61 tgcgttcatc accggcgacg gtgcgaagca tgcagatacg gggatgacgg tctgcgcgac
      121 ggacagccgc aaacacaaag acaaattcag ggtgtgaggg ggtaagggtt gtagccataa
      181 ggcagtctcc ttcgagtagc aaataactgc tatcgccgga gttcctacgc tcgatggcga
      241 tagcccagac gggggtagga ataccggcct cgaagaatac cggccagccc ggaggctgcc
      301 ccgcctgagc taccattgac tcagtggcat aacatgcgat tgcgaacagg atcatacctg
      361 cacggcaaac cacacgccac accataatct ggcgctctgt ggcgttgatt gcgacacaaa
      421 aaaagacgca tggcgcgtca tatgtcgcct tcgagttaca cgggttccta cgcccggctg
      481 ccgattttgc gacagcggaa aaactatatc cgcaaatgcc ggaaaaaggc aagccagaaa
      541 aagggacttt ttgcagaaca ggcatcatca tgcgtcatac ccccgttggc gtc
//
LOCUS       DAMZFT010000380          586 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.386, whole genome shotgun sequence.
ACCESSION   DAMZFT010000380 DAMZFT010000000
VERSION     DAMZFT010000380.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 586)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 586)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..586
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.386"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(<1..564)
                     /locus_tag="PN977_005429"
     CDS             complement(<1..564)
                     /locus_tag="PN977_005429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000980235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
                     /protein_id="HDI6045310.1"
                     /translation="MMMPALQKLPFSGLPFSGICGYSFSAAAKSAAGRENPSMLSATY
                     DAPCVFFCVAINATEHQIMVWRVVCRAGMILFAIACYATESMVAQAGQPPGWPVSDNA
                     GILTPVWAIAIERENSGDSVIYAVIGGCLMATTLTPSHPEFVFVFAAVRRADRHPRIC
                     MLRTVAGDERSARRSLVRDYVLSLAARL"
BASE COUNT          157 a          158 c          175 g           96 t
ORIGIN      
        1 cagacgggca gcaagggaga gcacatagtc acggacaagg gaacggcggg cgctgcgttc
       61 atcaccggcg acggtgcgaa gcatacagat acggggatga cggtctgcgc gacggacagc
      121 cgcaaacaca aagacaaatt cagggtgtga gggggtaagg gtcgtagcca taaggcaacc
      181 tccgataaca gcgtaaatga cgctatcgcc ggagttctca cgctcgatgg cgatagccca
      241 gacgggggtg agaataccgg cgttatcgga aaccggccag cccggaggct gccccgcctg
      301 agctaccatt gactcagtgg cataacatgc gattgcgaac aggatcatac ctgcacggca
      361 aaccacacgc cacaccataa tctggtgctc tgtggcgttg attgcgacac aaaaaaagac
      421 gcatggcgcg tcatatgtcg ccgataacat actcgggttc tcacgcccgg ctgccgattt
      481 tgcggcagcg gaaaaactat atccgcaaat gccggaaaaa ggcaagccag aaaaagggag
      541 tttttgcaga gcgggcatca tcatgcgtcg tacccccgtt tgcgtc
//
LOCUS       DAMZFT010000381          584 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.387, whole genome shotgun sequence.
ACCESSION   DAMZFT010000381 DAMZFT010000000
VERSION     DAMZFT010000381.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 584)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 584)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..584
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.387"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..>584
                     /locus_tag="PN977_005430"
     rRNA            <1..>584
                     /locus_tag="PN977_005430"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          164 a          127 c          178 g          115 t
ORIGIN      
        1 tgtataatgg gtcagcgact tatattctgt agcaaggtta accgaatagg ggagccgaag
       61 ggaaaccgag tcttaactgg gcgttaagtt gcagggtata gacccgaaac ccggtgatct
      121 agccatgggc aggttgaagg ttgggtaaca ctaactggag gaccgaaccg actaatgttg
      181 aaaaattagc ggatgacttg tggctggggg tgaaaggcca atcaaaccgg gagatagctg
      241 gttctccccg aaagctattt aggtagcgcc tcgtgaattc atctccgggg gtagagcact
      301 gtttcggcaa gggggtcatc ccgacttacc aacccgatgc aaactgcgaa taccggagaa
      361 tgttatcacg ggagacacac ggcgggtgct aacgtccgtc gtgaagaggg aaacaaccca
      421 gaccgccagc taaggtccca aagtcatggt taagtgggaa acgatgtggg aaggcccaga
      481 cagccaggat gttggcttag aagcagccat catttaaaga aagcgtaata gctcactggt
      541 cgagtcggcc tgcgcggaag atgtaacggg gctaaaccat gcac
//
LOCUS       DAMZFT010000382          580 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.388, whole genome shotgun sequence.
ACCESSION   DAMZFT010000382 DAMZFT010000000
VERSION     DAMZFT010000382.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 580)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 580)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..580
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.388"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            58..>580
                     /locus_tag="PN977_005431"
     CDS             58..>580
                     /locus_tag="PN977_005431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001304459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6045311.1"
                     /translation="MTYFKTHRDCYADGWNACRAAMLQSGNFRENKNSSTNNFREIAE
                     TSTNYPVIPSEVLSAILKVAKIRADFDDFDGDRRGIGDCLDEAEQELIVTINKYASQL
                     AAEPIATNDVREQTAVPPVPVIQADVAQAIEKLKRKLVECNRYNYCADAVKGVEYACH
                     AATLPRCHAAMLQG"
BASE COUNT          157 a          139 c          154 g          130 t
ORIGIN      
        1 gactcacctg cgccggttgt gccggatgaa atagacgtaa acgatgtacc aagggcggtg
       61 acatatttca agacacacag agattgttac gcagatggct ggaacgcctg ccgcgccgcc
      121 atgcttcagt ccggaaactt tcgggaaaac aagaattcgt caaccaataa ttttcgggaa
      181 atcgcggaaa cgtcaaccaa ctatccggta attcctagtg aggtgttgtc cgcaatcctg
      241 aaggttgcca agattcgtgc cgatttcgat gattttgacg gtgacaggcg aggtatcggt
      301 gattgtctgg atgaggctga gcaagagctt atcgttacca ttaacaaata tgccagtcag
      361 ttggcagcag aaccgatagc gactaatgac gttcgagagc aaacagccgt tccgccagtt
      421 cctgtaatac aggctgatgt cgcgcaggca attgaaaaac tcaaacggaa attagtggaa
      481 tgcaatcgct ataactactg cgcagatgca gttaagggcg ttgagtatgc ctgccacgct
      541 gccacgctgc cacgctgcca cgctgccatg cttcagggta
//
LOCUS       DAMZFT010000383          578 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.389, whole genome shotgun sequence.
ACCESSION   DAMZFT010000383 DAMZFT010000000
VERSION     DAMZFT010000383.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 578)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 578)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..578
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.389"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..>578
                     /gene="tuf"
                     /locus_tag="PN977_005432"
     CDS             <1..>578
                     /gene="tuf"
                     /locus_tag="PN977_005432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HDI6045312.1"
                     /translation="LELAGFLDSYIPEPERAIDKPFLLPIEDVFSISGRGTVVTGRVE
                     RGIIKVGEEVEIVGIKETQKSTCTGVEMFRKLLDEGRAGENVGVLLRGIKREEIERGQ
                     VLAKPGTIKPHTKFESEVYILSKDEGGRHTPFFKGYRPQFYFRTTDVTGTIELPEGVE
                     MVMPGDNIKMVVTLIHPIAMDDGLRFAIREGGR"
BASE COUNT          137 a          144 c          161 g          136 t
ORIGIN      
        1 ctggaactgg ctggcttcct ggattcttac attccggaac cagagcgtgc gattgacaag
       61 ccgttcctgc tgccgatcga agacgtattc tccatctccg gtcgtggtac cgttgttacc
      121 ggtcgtgtag aacgcggtat catcaaagtt ggtgaagaag ttgaaatcgt tggtatcaaa
      181 gagactcaga agtctacctg tactggcgtt gaaatgttcc gcaaactgct ggacgaaggc
      241 cgtgctggtg agaacgtagg tgttctgctg cgtggtatca aacgtgaaga aatcgaacgt
      301 ggtcaggtac tggctaagcc gggcaccatc aagccgcaca ccaagttcga atctgaagtg
      361 tacattctgt ccaaagatga aggcggccgt catactccgt tcttcaaagg ctaccgtccg
      421 cagttctact tccgtactac tgacgtgact ggtaccatcg aactgccgga aggcgtagag
      481 atggtaatgc cgggcgacaa catcaaaatg gttgttaccc tgatccaccc gatcgcgatg
      541 gacgacggtc tgcgtttcgc aatccgtgaa ggcggccg
//
LOCUS       DAMZFT010000384          576 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.390, whole genome shotgun sequence.
ACCESSION   DAMZFT010000384 DAMZFT010000000
VERSION     DAMZFT010000384.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 576)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 576)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..576
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.390"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..>576
                     /gene="iucD"
                     /locus_tag="PN977_005433"
     CDS             <1..>576
                     /gene="iucD"
                     /locus_tag="PN977_005433"
                     /EC_number="1.14.13.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent L-lysine N(6)-monooxygenase"
                     /protein_id="HDI6045313.1"
                     /translation="EAAFADEYFTPEYISGFSGLEEDIRHQLLDEQKMTSDGITADSL
                     LTIYRELYHRFEVLRKPRNIRLLPSRSVTTLESSGPGWKLLMEHHLDQGRESLESDVV
                     IFATGYRSALPQILPSLMPLITMHDKNTFKVRDDFTLEWSGPKENNIFVVNASMQTHG
                     IAEPQLSLMAWRSARILNRVMGRDLFDLSMPP"
BASE COUNT          146 a          136 c          143 g          151 t
ORIGIN      
        1 gaggctgctt ttgctgatga gtattttaca cctgaatata tttcaggctt ctccggactg
       61 gaggaagata ttcgccatca gttactggat gagcagaaaa tgacatcgga tggcatcact
      121 gccgattctt tactgaccat ttatcgtgag ttgtaccacc gttttgaagt tctgagaaaa
      181 ccaagaaata tccgtctgct acccagccgc tcggtaacaa ctctggaaag tagtggtcct
      241 ggctggaagt tgctgatgga gcatcatctg gatcagggca gggagagcct ggaaagtgat
      301 gtggtgattt tcgccacagg ttaccgttct gcgttgccac aaatacttcc ctcactgatg
      361 ccgctgatca ccatgcacga taagaacacc tttaaagtgc gtgatgactt cactctggaa
      421 tggagtggcc cgaaagagaa caacatcttc gtggtcaacg ccagtatgca aacccatggc
      481 atcgccgaac ctcagctcag cctgatggca tggagatctg cacgtattct taatcgtgta
      541 atgggacgtg atttattcga tctcagtatg ccgccc
//
LOCUS       DAMZFT010000385          575 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.391, whole genome shotgun sequence.
ACCESSION   DAMZFT010000385 DAMZFT010000000
VERSION     DAMZFT010000385.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 575)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 575)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..575
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.391"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..>575
                     /locus_tag="PN977_005434"
     CDS             <1..>575
                     /locus_tag="PN977_005434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1281 domain-containing protein"
                     /protein_id="HDI6045314.1"
                     /translation="SEQCPGVTAAGRGAVSTENIAFTRWLTHLQNGVLLDEQNCLMLH
                     ELWLQSGTGQRRWEGLPDDVRETITVHFTAKRGDWCDIWGSEDVSVWWNRLCDNVVPE
                     KTMPFDLLTVLPTRLDVEVNGFNGGVLNGVPSAYHWYTERYGVKWPCGYDLNISSQGD
                     NCIQVDFDTPWCQPESDVVAALSRRFGCTLE"
BASE COUNT          128 a          130 c          183 g          134 t
ORIGIN      
        1 tctgaacagt gccccggagt gacggctgcc ggacgtggtg ctgtatcgac ggaaaatatc
       61 gcgttcaccc gctggctgac acacctgcag aacggtgtac tgctggatga gcaaaactgc
      121 ctgatgctgc atgaactctg gctgcagagc ggtactggac agcgtcgctg ggaaggatta
      181 ccggatgatg tcagggaaac catcactgtg catttcaccg caaaaagagg tgactggtgt
      241 gacatctggg gtagcgagga tgtatcggtg tggtggaacc gtctgtgtga caacgtagtg
      301 ccggaaaaaa ccatgccgtt tgacctgctg acggttctgc cgacccgcct ggatgttgaa
      361 gtgaatggct ttaatggtgg tgttctgaac ggtgttcctt ctgcatatca ctggtataca
      421 gaacggtatg gcgtgaagtg gccttgtggg tatgacctga atatcagtag tcagggagac
      481 aactgcattc aggtggattt cgacacgccg tggtgtcagc cggaaagcga cgttgttgca
      541 gcattaagcc gccgtttcgg ctgcacgctg gagca
//
LOCUS       DAMZFT010000386          565 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.392, whole genome shotgun sequence.
ACCESSION   DAMZFT010000386 DAMZFT010000000
VERSION     DAMZFT010000386.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 565)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 565)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..565
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.392"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            58..282
                     /locus_tag="PN977_005435"
     CDS             58..282
                     /locus_tag="PN977_005435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001477406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shufflon protein B"
                     /protein_id="HDI6045315.1"
                     /translation="MEIVKVTASKLRWPGATATCPMGKKVIGGGAECSSGIGFIWLVR
                     SIPVNNNAWYGYCDTTEHIIGKITVHAICQ"
BASE COUNT          153 a          136 c          129 g          147 t
ORIGIN      
        1 gattggcact ggcctccgtg ccaatccggt tcgtggaaaa atgcaggaaa aggcgatatt
       61 gaaatcgtca aagtaacagc atcaaaactc cgctggcctg gtgctactgc cacctgccca
      121 atgggtaaga aggtgatcgg gggtggtgca gaatgcagct ctggaattgg ctttatctgg
      181 cttgtccgtt caatacctgt aaacaataat gcctggtatg gctattgcga tacgacagaa
      241 cacatcattg gaaaaataac tgtacatgcc atttgccaat gacaaagtga tttactggca
      301 aaccacaatg taactacatt tccccgtcag gtcgttcctg tttgtaccgc cgtgaaaatt
      361 gtattcatcg tagtaaccac acttcacaat cctgttcgaa tcatatgtcg caacagaacc
      421 tgcgaagtaa tttggcttcc agccatgcgg gttttcagca ttgctgacgc tccaggtaca
      481 ctggctggca gaaaaaccag acggcaaagg tagtcgttct ccatttgcga tttttcctgt
      541 cagaacagtg cttcctgata cccta
//
LOCUS       DAMZFT010000387          563 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.393, whole genome shotgun sequence.
ACCESSION   DAMZFT010000387 DAMZFT010000000
VERSION     DAMZFT010000387.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 563)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 563)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..563
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.393"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..>563
                     /locus_tag="PN977_005436"
     CDS             <1..>563
                     /locus_tag="PN977_005436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HDI6045316.1"
                     /translation="NAKERPRFGMVYPTGWLRNGQPTPRSDKMSGEYVSSEVVNWLDN
                     KKDSKPFFLYVAFTEVHSPLASPKKYLDMYSQYMSDYQKQHPDLFYGDWADKPWRGTG
                     EYYANISYLDAQVGKVLDKIKAMGEEDNTIVIFTSDNGPVTREARKVYELNLAGETDG
                     LRGRKDNLWEGGIRVPAIIKYGKHLPKG"
BASE COUNT          164 a          116 c          144 g          139 t
ORIGIN      
        1 aatgcgaagg agcgtccccg ttttggcatg gtctatccaa cgggctggtt gcgtaacggg
       61 caacccacac cacgttccga taaaatgagt ggtgagtatg tcagttcgga agtcgtcaac
      121 tggttggata acaaaaagga cagtaagcct ttcttccttt atgtcgcttt taccgaagtg
      181 cacagtcccc tggcttcgcc caaaaaatac ctcgacatgt actcacaata tatgagcgac
      241 tatcagaagc agcatcctga tttattttat ggcgactggg cggataaacc ctggcgtggt
      301 acaggagaat attatgccaa catcagttat ctggatgctc aggttggaaa agtactggat
      361 aaaatcaaag cgatgggtga agaagataac accatcgtta tttttaccag tgataacgga
      421 ccagtaacgc gtgaagcgcg caaagtttat gaactgaatt tggcagggga aactgatgga
      481 ttacgtggtc gcaaggataa tctctgggaa ggtggcatcc gtgttccagc gattattaaa
      541 tacggaaagc atcttccaaa ggg
//
LOCUS       DAMZFT010000388          560 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.394, whole genome shotgun sequence.
ACCESSION   DAMZFT010000388 DAMZFT010000000
VERSION     DAMZFT010000388.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 560)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 560)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..560
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.394"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            1..485
                     /locus_tag="PN977_005437"
                     /pseudo
     CDS             1..485
                     /locus_tag="PN977_005437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343613.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          161 a          131 c          145 g          123 t
ORIGIN      
        1 aatagcgtgt acccgcagac gcatattcag ctctgcatca tccatatggt gcgcaacagc
       61 ctgaaattcg tgtcgtggaa ggattacaaa gccgtcacca gcgggctgaa agcggtatat
      121 caggccccga cagaggaaac ggcgttgatg gcgttggata agtttgcagg cgtctgggat
      181 gaaaaatacc cgcaaatcag caagagctgg cgcacacact gggaaaatct caacaccttc
      241 ttcggttacc caccggatat ccgcaaggcc atctacacga cgaatgccat cgaatcgctg
      301 aacagtgtca ttcggcaggc gataaagaag cgcaaggtgt tcccaacgga cgactcggtg
      361 cgaaaagtga tttatctggc gatacagtcg gcgtcaaaaa atggagtatg ccaatccaga
      421 actggcggct ggcaatgagc cgttttatta tcgagttcgg tgaccgcctt agcgagcacc
      481 tttaatacgg cggcagttac acagaattat ttacagggtc attctgaatt tagtgcagaa
      541 ttaaaatatg tgttcaaaat
//
LOCUS       DAMZFT010000389          560 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.395, whole genome shotgun sequence.
ACCESSION   DAMZFT010000389 DAMZFT010000000
VERSION     DAMZFT010000389.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 560)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 560)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..560
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.395"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            93..338
                     /locus_tag="PN977_005438"
     CDS             93..338
                     /locus_tag="PN977_005438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000984207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ogr/Delta-like zinc finger family protein"
                     /protein_id="HDI6045317.1"
                     /translation="MMRCPFCRHSAHTRTSRYVSDNVKESYLQCQNIYCSATFKTHES
                     ICAVIRSPVTEEKPAPASTAPAVVRKVKGCYSSPFNH"
     gene            353..>560
                     /locus_tag="PN977_005439"
     CDS             353..>560
                     /locus_tag="PN977_005439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Polarity suppression protein"
                     /protein_id="HDI6045318.1"
                     /translation="MTTMSLQQAFEVCQNNKAAWLQRKNELAAAEQEYLRLLSGEGRN
                     VSRLDELRNIIEVRKWQVNQAAGRY"
BASE COUNT          157 a          134 c          156 g          113 t
ORIGIN      
        1 tatgaggtgg caggcacacc ggcacagcgc aaggtgtggc aggacaaaat agaccagcag
       61 ggagcagagc ttaaggccag agggctgctg tcatgatgcg ctgccctttc tgtcgtcatt
      121 cagcgcatac ccgcaccagc cggtatgtga gtgacaatgt caaagaaagt tatctgcagt
      181 gccagaatat ttactgttcg gcgacattta aaacgcatga gtcaatttgt gccgtgattc
      241 gttctccggt cacggaggaa aaaccagcac cggcaagcac agcaccggct gttgtccgaa
      301 aagttaaagg ctgttacagc tcaccattca accattaatc aggagacggg gtgtgaccac
      361 aatgtcatta cagcaggctt ttgaggtctg ccagaataac aaagcggcat ggctgcaacg
      421 caaaaatgag ctggctgcag ccgaacagga atatctgcgg cttctgtccg gggaaggcag
      481 aaacgtcagc cgcctggacg aattacgcaa tattatcgaa gtcagaaaat ggcaggtgaa
      541 tcaggccgcc ggtcgttata
//
LOCUS       DAMZFT010000390          559 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.396, whole genome shotgun sequence.
ACCESSION   DAMZFT010000390 DAMZFT010000000
VERSION     DAMZFT010000390.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 559)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 559)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..559
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.396"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..304
                     /locus_tag="PN977_005440"
     CDS             <1..304
                     /locus_tag="PN977_005440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020077623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="D-galactonate dehydratase"
                     /protein_id="HDI6045319.1"
                     /translation="APHCPLGPIALAACLHIDFVSYNAVLQEQSMGIHYNKGAELLDF
                     VKNKEDFSMVGGFFKPLTKPGLGVEIDEAKVIEFSKNAPDWRNPLWRHEDNSVAEW"
     gene            379..>559
                     /locus_tag="PN977_005441"
     CDS             379..>559
                     /locus_tag="PN977_005441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDI6045320.1"
                     /translation="MVSGFAMPKIRRKLAMDIPVNAAKPGRRRYLTLVMIFITVVICY
                     VDRANLAVAAAHIQEE"
BASE COUNT          147 a          136 c          143 g          133 t
ORIGIN      
        1 tgcgccgcac tgtccgctcg gaccgattgc actggcagct tgcctgcata tcgactttgt
       61 ttcctataac gccgtacttc aggaacaaag tatgggcatt cattacaaca aaggcgcgga
      121 gttactcgac tttgtgaaaa acaaagagga cttcagcatg gtcggcggct tctttaaacc
      181 gctaacgaaa ccgggcttag gcgtggaaat cgacgaagct aaagttattg agttcagtaa
      241 aaatgccccg gactggcgta atccgctctg gcgtcatgaa gataacagcg tagcagagtg
      301 gtaattcctg ccacgtaagc ccctcatcgg gcactaaaac agcaatacaa aaatataacc
      361 ctctgtaaat tacagggcat ggtgagcggc ttcgctatgc ccaaaatcag gagaaagctc
      421 gctatggata ttcccgttaa tgcagcaaag ccggggcgtc ggcgttatct gacgctggtg
      481 atgatcttta ttacggtggt catttgttat gttgaccgcg ctaacctggc cgtggctgcc
      541 gcccatattc aggaagagt
//
LOCUS       DAMZFT010000391          558 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.397, whole genome shotgun sequence.
ACCESSION   DAMZFT010000391 DAMZFT010000000
VERSION     DAMZFT010000391.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 558)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 558)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..558
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.397"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            247..>558
                     /locus_tag="PN977_005442"
     CDS             247..>558
                     /locus_tag="PN977_005442"
                     /inference="COORDINATES: protein motif:HMM:NF024673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HDI6045321.1"
                     /translation="MQQFRHWQNRLNTIDPARIKLYSETVRVWNQSRGSAGARALADM
                     LIQNGIPMSSCRARRLMKHLNLRSAQPGKYQYKNARQEHTSLPSLLECRFSVLEAEQV
                     WI"
BASE COUNT          141 a          135 c          157 g          125 t
ORIGIN      
        1 actggggggc atgggtacgt cagctcaggc gggaacgtca ggataccacg tcttctgcat
       61 caccactcac atcagagtag caacgtattc atgaactgga aaagcaggtt cgtcgcctgg
      121 aggaacaaaa aacgatatta aaaagctaca gtgctcttga tatccttccc actgaacggt
      181 ttacgatagt taccagactg agcggcgaca cacgattgtc agtctttgcg ctgctctgga
      241 tgctcattgc agcagttccg gcactggcag aatcggctca atacgattga tccggcgcga
      301 atcaaattgt atagcgaaac agtccgggtc tggaaccaga gtcggggttc cgcaggtgcg
      361 cgtgctctgg cagatatgct gattcaaaat ggtatcccga tgagcagttg tcgtgccagg
      421 cggctgatga agcatctgaa cctgagaagt gctcagcccg gaaaatatca gtacaaaaat
      481 gcccgccagg agcataccag cctgccgagt ctgcttgagt gtcggttttc tgtgctggag
      541 gccgagcagg tatggatt
//
LOCUS       DAMZFT010000392          557 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.398, whole genome shotgun sequence.
ACCESSION   DAMZFT010000392 DAMZFT010000000
VERSION     DAMZFT010000392.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 557)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 557)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..557
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.398"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(<1..524)
                     /locus_tag="PN977_005443"
     CDS             complement(<1..524)
                     /locus_tag="PN977_005443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001323508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tat pathway signal sequence protein"
                     /protein_id="HDI6045322.1"
                     /translation="MLRTTVLAGEVIIQENNRMLKRRDAFLKKSALAVSVALLLSAQA
                     QAVLTGPVDGNSSSLLIGENSFITNLTGTVNNTFLLGGGAFNLDSPGSLQFGSFSEVY
                     NSPHSVTLGRDAGQAESKYGVAIGKSAEVFNSQHSVAIGGWAGIENSSGSVALGHGSQ
                     VSGENNVVSVGAGP"
BASE COUNT          167 a          150 c          103 g          137 t
ORIGIN      
        1 tcaggcccgg caccaacaga taccacgtta ttttctcccg acacctgtga gccatggccc
       61 agagctacag agcctgaaga attttctatt ccagcccatc ccccaatagc aacactatgc
      121 tgtgaattaa atacctcagc gctcttacct atggcaacac catattttga ctcagcctga
      181 ccggcatccc ttcccagggt gacactgtgt ggagaattat acacttccga aaaagagcca
      241 aactgaagac ttcctggtga atccaggtta aaagcgccac ctcccaaaag gaaagtgtta
      301 ttaactgtac cagttaaatt cgtaataaaa gagttttctc caatcagtaa gctggaagaa
      361 ttaccatcaa caggccctgt caaaacagcc tgagcctgag ccgagagcag cagggccaca
      421 ctcactgcca gcgcgctttt ctttaaaaaa gcatcacgtc ttttaagcat tctgttattc
      481 tcctgtatta ttacttcacc agccagaacg gtagtgcgga gcaaaaatac agtgataaga
      541 atcctctctt attaatt
//
LOCUS       DAMZFT010000393          552 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.399, whole genome shotgun sequence.
ACCESSION   DAMZFT010000393 DAMZFT010000000
VERSION     DAMZFT010000393.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 552)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 552)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..552
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.399"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..>552
                     /locus_tag="PN977_005444"
     CDS             <1..>552
                     /locus_tag="PN977_005444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FHIPEP family type III secretion protein"
                     /protein_id="HDI6045323.1"
                     /translation="GDAYPGRWLAINPGTAAGTLPGEATVDPAFGLNAIWIESALKEQ
                     AQIQGYTVVEASTVVATHLNHLISQHAAELFGRQEAQQLLDRVAQEMPKLTEDLVPGV
                     VTLTTLHKVLQNLLDEKVPIRDMRTILETLAEHAPIQSDPHELTAVVRVALGRAITQQ
                     WFPGKDEVHVIGLDTPLERLLLQ"
BASE COUNT          124 a          145 c          162 g          121 t
ORIGIN      
        1 gtggtgatgc ttatccgggg cgttggttgg cgattaaccc tggtaccgct gccgggacgt
       61 tacctggtga ggcgaccgtc gatccggcat ttggcctgaa tgctatctgg attgaaagtg
      121 cgcttaaaga acaggcgcag attcagggat acaccgtggt tgaggccagc acggtggtgg
      181 caacgcatct taaccacctc attagccagc atgccgcaga gctgtttggt cgtcaggagg
      241 cgcaacagct gctggatcgc gtcgcccagg agatgccaaa gctgacggaa gatctcgttc
      301 ctggcgtcgt cacgctcacc acactgcata aagtgctgca aaatctgctc gatgaaaaag
      361 taccgattcg cgatatgcgc actattctcg aaacgctggc ggaacatgcg cccatccaaa
      421 gcgatccaca tgaattaacc gccgtcgtgc gcgtggcgtt gggacgggcg attacccagc
      481 agtggtttcc tggcaaagat gaagtccatg ttattggcct cgatacaccg ctggaacgtt
      541 tgttactaca gg
//
LOCUS       DAMZFT010000394          528 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.400, whole genome shotgun sequence.
ACCESSION   DAMZFT010000394 DAMZFT010000000
VERSION     DAMZFT010000394.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 528)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 528)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..528
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.400"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            33..317
                     /locus_tag="PN977_005445"
     CDS             33..317
                     /locus_tag="PN977_005445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415994.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antidote protein"
                     /protein_id="HDI6045324.1"
                     /translation="MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTG
                     KAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAEKTVDVSRLRRLVTQ"
     gene            complement(368..>528)
                     /locus_tag="PN977_005446"
     CDS             complement(368..>528)
                     /locus_tag="PN977_005446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310107.1"
                     /note="cytochrome b556(FDO) component; heme containing;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="formate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HDI6045325.1"
                     /translation="ILIHMYMAFWVKGSIKGMIEGKVSRRWAKKHHPRWYREIEKAEA
                     KKESEEGI"
BASE COUNT          137 a          136 c          124 g          131 t
ORIGIN      
        1 gtgaaggaac gctactgaga gtaaagacac tgatgaaaat ggccaatcat ccccgcccgg
       61 gggacattat tcaggaatca ctggacgaac ttaatgtcag cctgcgcgag tttgccagag
      121 caatggaaat tgctccctca acggcaagcc gattgctgac cggaaaagca gctttgacgc
      181 cagaaatggc aattaaactt tccgtggtga tcggcagttc gccgcaaatg tggctgaatc
      241 tgcaaaacgc ctggagcctg gcagaggctg aaaaaacggt ggatgtgtcg cgactgcgcc
      301 gtctggttac gcaataaaaa aatggcgctt cttaaagcgc cattttcagc ttaaagttca
      361 aaagagatta tatcccttct tcactctctt ttttcgcttc tgccttctcg atttcacgat
      421 accagcgcgg atggtgtttc ttcgcccagc gacggctcac cttcccttcg atcatccctt
      481 taatcgatcc tttcacccag aatgccatat acatatggat caggatgg
//
LOCUS       DAMZFT010000395          523 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.401, whole genome shotgun sequence.
ACCESSION   DAMZFT010000395 DAMZFT010000000
VERSION     DAMZFT010000395.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 523)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 523)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..523
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.401"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..219
                     /locus_tag="PN977_005447"
     CDS             <1..219
                     /locus_tag="PN977_005447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HDI6045326.1"
                     /translation="DDDDDETWVLFNVMNGNRAEMSPEAAGIAACLMTYSHHACRTEC
                     YAMTVHYYRLRDYALQHPECSAIMRIID"
     gene            235..>523
                     /locus_tag="PN977_005448"
     CDS             235..>523
                     /locus_tag="PN977_005448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RadC family protein"
                     /protein_id="HDI6045327.1"
                     /translation="MQQLSFLPGEMTPGERSLILRALKTLDRHLHEPGVPFTSTRAAR
                     EWLILNMAGLEREEFRVLYLNNQNQLIAGETLFTGTINRTEVHPREVIKRAL"
BASE COUNT          124 a          151 c          141 g          107 t
ORIGIN      
        1 gatgacgatg atgacgaaac atgggtactg ttcaatgtca tgaacggtaa ccgcgctgaa
       61 atgagcccgg aagccgccgg cattgctgcc tgtctgatga cgtacagtca tcatgcctgt
      121 cgtacagagt gttatgccat gacggtccat tattaccggc tgcgggatta cgccctgcag
      181 catccggaat gcagcgccat tatgcgcatc atcgactgaa tggagagaag cacaatgcaa
      241 caactttcct ttctgccagg agagatgacg cccggcgagc gcagcctcat tctgcgggcc
      301 ctgaaaaccc tggaccgcca tcttcatgaa cccggcgtgc ccttcacctc cacccgtgcg
      361 gcacgggaat ggctgattct gaacatggcg ggactggagc gtgaagaatt ccgggtgctg
      421 tatctgaata accagaatca gctgattgcc ggtgaaaccc tcttcaccgg caccatcaac
      481 cgcacggaag tccatccccg ggaagtgatt aaacgcgccc tgt
//
LOCUS       DAMZFT010000396          520 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.402, whole genome shotgun sequence.
ACCESSION   DAMZFT010000396 DAMZFT010000000
VERSION     DAMZFT010000396.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 520)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 520)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..520
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.402"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..>520
                     /locus_tag="PN977_005449"
     rRNA            <1..>520
                     /locus_tag="PN977_005449"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          128 a          121 c          161 g          110 t
ORIGIN      
        1 agagatggat tggtgccttc gggaactgtg agacaggtgc tgcatggctg tcgtcagctc
       61 gtgttgtgaa atgttgggtt aagtcccgca acgagcgcaa cccttatcct ttgttgccag
      121 cggtccggcc gggaactcaa aggagactgc cagtgataaa ctggaggaag gtggggatga
      181 cgtcaagtca tcatggccct tacgaccagg gctacacacg tgctacaatg gcgcatacaa
      241 agagaagcga cctcgcgaga gcaagcggac ctcataaagt gcgtcgtagt ccggattgga
      301 gtctgcaact cgactccatg aagtcggaat cgctagtaat cgtggatcag aatgccacgg
      361 tgaatacgtt cccgggcctt gtacacaccg cccgtcacac catgggagtg ggttgcaaaa
      421 gaagtaggta gcttaacctt cgggagggcg cttaccactt tgtgattcat gactggggtg
      481 aagtcgtaac aaggtaaccg taggggaacc tgcggttgga
//
LOCUS       DAMZFT010000397          520 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.403, whole genome shotgun sequence.
ACCESSION   DAMZFT010000397 DAMZFT010000000
VERSION     DAMZFT010000397.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 520)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 520)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..520
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.403"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..>520
                     /locus_tag="PN977_005450"
     rRNA            <1..>520
                     /locus_tag="PN977_005450"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          130 a          117 c          170 g          103 t
ORIGIN      
        1 gtaaagttaa tacctttgct cattgacgtt acccgcagaa gaagcaccgg ctaactccgt
       61 gccagcagcc gcggtaatac ggagggtgca agcgttaatc ggaattactg ggcgtaaagc
      121 gcacgcaggc ggtttgttaa gtcagatgtg aaatccccgg gctcaacctg ggaactgcat
      181 ctgatactgg caagcttgag tctcgtagag gggggtagaa ttccaggtgt agcggtgaaa
      241 tgcgtagaga tctggaggaa taccggtggc gaaggcggcc ccctggacga agactgacgc
      301 tcaggtgcga aagcgtgggg agcaaacagg attagatacc ctggtagtcc acgccgtaaa
      361 cgatgtcgac ttggaggttg tgcccttgag gcgtggcttc cggagctaac gcgttaagtc
      421 gaccgcctgg ggagtacggc cgcaaggtta aaactcaaat gaattgacgg gggcccgcac
      481 aagcggtgga gcatgtggtt taattcgatg caacgcgaag
//
LOCUS       DAMZFT010000398          519 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.404, whole genome shotgun sequence.
ACCESSION   DAMZFT010000398 DAMZFT010000000
VERSION     DAMZFT010000398.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 519)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 519)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..519
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.404"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..192
                     /locus_tag="PN977_005451"
     CDS             <1..192
                     /locus_tag="PN977_005451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDI6045328.1"
                     /translation="TIKRDYISIMPKPDGLTAAKNLAEAFEHYNEWHPHSALGYRSPR
                     EYLRQQASNGLSDNRCLEI"
     gene            205..321
                     /locus_tag="PN977_005452"
                     /pseudo
     CDS             205..321
                     /locus_tag="PN977_005452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743396.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            337..486
                     /locus_tag="PN977_005453"
                     /pseudo
     CDS             337..486
                     /locus_tag="PN977_005453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558245.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          149 a          116 c          142 g          112 t
ORIGIN      
        1 acgataaagc gtgactacat cagtatcatg cctaaaccag acgggttaac ggcagcaaag
       61 aaccttgcag aggcgttcga gcattataac gaatggcatc cgcatagtgc actgggttat
      121 cgctcgccac gggaatatct gcggcagcag gccagtaatg ggttaagtga taacaggtgt
      181 ctggaaatat aggggcaaat ccacctgaat ctgaggcagc acctggcacg gctgggacgg
      241 aagtcgctgt cgttttcaaa atcggtggag ctgcatgaca aagtcatcag gcattatctg
      301 aacataaaac actatcaata agttggagtc attaccgtgt tcccgacaga cgactcggtg
      361 aaaaaagtgg tgtggctggc aatccagtct gcgtcccaga aatggacgat gccgttgaag
      421 gactggcgaa tggcaatgag ccgctttatt atcgagttcg gtgaccgcct ggacggtcac
      481 ttctgagaaa aggcatttac acagaatcct aaacaggct
//
LOCUS       DAMZFT010000399          512 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.405, whole genome shotgun sequence.
ACCESSION   DAMZFT010000399 DAMZFT010000000
VERSION     DAMZFT010000399.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 512)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 512)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..512
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.405"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            4..>512
                     /locus_tag="PN977_005454"
     CDS             4..>512
                     /locus_tag="PN977_005454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009450597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6045329.1"
                     /translation="MSNRFYMMCLRETVGNNASFHCHNGNGYSSDIDRAHVYTLEEAQ
                     KAWNCGRDIDQPVCADSVDAMAVWHVDCQYIPTESLIGSDCTAYVAYKKGSWNGNDVY
                     WLQHGGLPTDDFSKATIFSVANKNEPGIVWLPFSIADAAKRRTFNINNFNRRTMVQGA
                     GLVMPDWLK"
BASE COUNT          138 a          111 c          132 g          131 t
ORIGIN      
        1 ccagtgagta accgttttta catgatgtgc ttgcgtgaaa ctgtgggtaa taacgcctca
       61 ttccattgcc ataacggcaa tggttacagt tctgatatcg atcgcgctca tgtttacacg
      121 ctggaagaag cccaaaaagc ctggaattgt gggcgagata tcgatcagcc tgtttgtgcc
      181 gatagtgtgg atgcaatggc tgtgtggcac gttgattgcc agtacatccc tacagaaagc
      241 ctgattgggt cagattgcac tgcgtatgtg gcctacaaaa aaggtagctg gaacggcaac
      301 gatgtttact ggcttcaaca cggtggattg ccaacagatg acttcagtaa agcgaccatc
      361 tttagcgtcg ccaacaaaaa cgaaccagga atagtttggt tgccattttc cattgctgat
      421 gcagcaaagc gccggacgtt caatatcaat aactttaacc gcagaacaat ggttcagggc
      481 gcaggtttgg tcatgcctga ctggttgaaa ga
//
LOCUS       DAMZFT010000400          502 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.406, whole genome shotgun sequence.
ACCESSION   DAMZFT010000400 DAMZFT010000000
VERSION     DAMZFT010000400.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 502)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 502)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..502
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.406"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..>502
                     /locus_tag="PN977_005455"
     CDS             <1..>502
                     /locus_tag="PN977_005455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011069568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HDI6045330.1"
                     /translation="QVYYRDESLRFYPFPTVNANKQVTAFSSSQQDTDQYGMKLTLNS
                     KPMDGWQITWGLDADHERFTSNQMFFDLAQASASGGLNNKKIYTTGRYPSYDITNLAA
                     FLQSGYDINNLFTLNGGVRYQYTENKIDDFIGYAQQRQIAAGKATSADAIPGGSVDYD
                     NFLFNAG"
BASE COUNT          124 a          149 c          123 g          106 t
ORIGIN      
        1 caggtttact accgcgatga gtcgttgcga ttctacccgt tcccgacggt aaatgcgaac
       61 aaacaggtga cggctttctc ttcgtcacag caggacaccg accagtacgg catgaaactg
      121 actctgaaca gcaaaccgat ggacggctgg caaatcacct gggggctgga tgctgatcat
      181 gagcgcttta cctccaacca gatgttcttc gacctggctc aggcaagcgc ttccggaggg
      241 ctgaacaaca agaagattta caccaccggg cgctatccgt cgtatgacat caccaacctg
      301 gcggccttcc tgcaatcagg ctatgacatc aataatctct ttaccctcaa cggtggcgta
      361 cgctatcagt acactgaaaa caagattgat gatttcatcg gctacgcgca gcaacggcag
      421 attgccgccg ggaaggctac atccgccgac gccattcctg gcggctcagt cgattacgac
      481 aacttcctgt tcaacgccgg tc
//
LOCUS       DAMZFT010000401          493 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.407, whole genome shotgun sequence.
ACCESSION   DAMZFT010000401 DAMZFT010000000
VERSION     DAMZFT010000401.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 493)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 493)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..493
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.407"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(<1..>493)
                     /locus_tag="PN977_005456"
     CDS             complement(<1..>493)
                     /locus_tag="PN977_005456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDI6045331.1"
                     /translation="FYLPFAFIIIVLLVVYLMTTRVFRRQFSEMTELVNTLAFLPDST
                     DQIEALKIREGDAKEIISIKNSIAEMKDAEIERSNKLLSLISYDQESGFIKNMAIIES
                     NNNQYLAVGIIKLCGLEAVEAVFGVDERNKIVRKLCQRIAEKYAQCCDIVTFNADLYL
                     LLCR"
BASE COUNT          148 a          120 c           91 g          134 t
ORIGIN      
        1 ccggcacagg agtaaataga gatcggcatt aaatgtcacg atatcgcagc attgcgcata
       61 tttctcggca attcgctgac acaattttct gacgatttta ttgcgttcat caacaccaaa
      121 caccgcttcc acggcttcca gaccacacag tttgatgatc cccacagcca gatactgatt
      181 attgttggac tcaataatcg ccatattttt aataaaaccg ctttcctgat cgtaagagat
      241 cagtgagagc aatttatttg accgttcaat ttcggcatct ttcatttccg cgatcgaatt
      301 tttgatgctg ataatctctt tcgcatcgcc ttcgcgaatt ttcagagcct cgatctgatc
      361 cgttgagtcg ggcaaaaacg ccagcgtatt aaccagctcg gtcatttcag aaaattgccg
      421 acggaacaca cgagtggtca ttaaataaac caccaacaat acgataataa tgaacgcaaa
      481 cggcagataa aaa
//
LOCUS       DAMZFT010000402          476 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.408, whole genome shotgun sequence.
ACCESSION   DAMZFT010000402 DAMZFT010000000
VERSION     DAMZFT010000402.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 476)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 476)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..476
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.408"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..>476
                     /locus_tag="PN977_005457"
     CDS             <1..>476
                     /locus_tag="PN977_005457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001260625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HDI6045332.1"
                     /translation="WQGVRVVTTETLARGYGTETNNIKVNYTRNADRFEEGKHYFLLT
                     GSKLKELKDKVTQSNLVAPRAKHLTLWTERGAARHAKMLETDQAWAFFEKLEDSYFRQ
                     KEQQPIAIPQTLPEALRLAAELAEQKQLLEQKAHQLNQQLVAAAPKVEFADRVSVA"
BASE COUNT          146 a          123 c          116 g           91 t
ORIGIN      
        1 tggcaggggg tgcgtgtagt gacaaccgag actcttgcta gagggtatgg gaccgaaact
       61 aataacatca aagttaacta tacgcgtaat gctgatcgct ttgaggaagg taaacattac
      121 tttctactaa ctggttcaaa attaaaagaa ttgaaggaca aggttactca aagtaactta
      181 gtcgcaccgc gagcaaagca cctcactctc tggacggaac gcggcgcagc ccgccacgct
      241 aaaatgctcg aaaccgatca ggcatgggca ttctttgaaa aactggaaga cagctacttc
      301 cgacaaaaag aacagcaacc gatcgcaatc ccccagacgc ttcctgaagc tctgcgcctg
      361 gctgccgaac tggctgaaca aaagcaactt ctggaacaga aagcccacca gctaaatcag
      421 cagctggtgg ccgcagcccc taaagtcgag tttgccgacc gggtatcagt ggccaa
//
LOCUS       DAMZFT010000403          465 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.409, whole genome shotgun sequence.
ACCESSION   DAMZFT010000403 DAMZFT010000000
VERSION     DAMZFT010000403.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 465)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 465)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..465
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.409"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            300..>465
                     /locus_tag="PN977_005458"
     CDS             300..>465
                     /locus_tag="PN977_005458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000949004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HDI6045333.1"
                     /translation="MLSIKKVTMLLGCLVLTCSIAFQASAAEKFKVITTFTIIADMAK
                     NVAGDAAEVSS"
BASE COUNT          131 a          110 c           76 g          148 t
ORIGIN      
        1 atcccttcat ttagtgactg cccctgggtt tttcatgctc tcctgcgtcc tagagaactt
       61 gccaataacc tacattattt ttgctttgcc attaccaatc gtcctgaaca agttcatttt
      121 aaagccaaag cgctgcccaa cttttatcct caataccaca tcgtcagcag gaaataacct
      181 aatccctgtg ttgggatctt ataatcgcta atgataatta tttacatttt atagcttctg
      241 ctataaaaca tagtagttgc tatgtttatt ctaattttgc tcactatagg tactaaatca
      301 tgctctcaat aaaaaaagta accatgctct tggggtgcct agtcctcacc tgctcgatag
      361 catttcaggc gagtgcagct gaaaaattca aggtcattac aacttttacc atcatcgcag
      421 atatggccaa aaacgtggct ggagatgctg cagaagtctc atcca
//
LOCUS       DAMZFT010000404          465 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.410, whole genome shotgun sequence.
ACCESSION   DAMZFT010000404 DAMZFT010000000
VERSION     DAMZFT010000404.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 465)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 465)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..465
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.410"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..>465
                     /gene="sitB"
                     /locus_tag="PN977_005459"
     CDS             <1..>465
                     /gene="sitB"
                     /locus_tag="PN977_005459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="iron/manganese ABC transporter ATP-binding
                     protein SitB"
                     /protein_id="HDI6045334.1"
                     /translation="ALRDASFTVPGGSIAALVGVNGSGKSTLFKAIMGFVRLTTGKIS
                     VLGIPTRQALQKNLIAYVPQSEEVDWSFPVLVEDVVMMGRYGHMGMLRIANKRDRQVV
                     TDALERVDMVDFRHRQIGELSGGQKKRVFLARAIAQQGDVILLDEPFTGVDV"
BASE COUNT          105 a          101 c          140 g          119 t
ORIGIN      
        1 cagcactgcg cgatgcatcc ttcacagtac caggcggatc tattgccgct ctggttgggg
       61 taaacggttc cggaaaatcg acgttattta aagcgattat gggattcgtg cgtctgacta
      121 ccggaaagat atctgttctg ggtattccca cgcggcaggc tctgcagaaa aacctgattg
      181 cctacgtccc gcagtcagaa gaagtggact ggtcctttcc tgtactggtg gaagatgtgg
      241 tgatgatggg ccgctatggg catatgggta tgttgcgtat agccaataag agggatcgtc
      301 aggttgtcac cgatgcgctg gaacgtgtcg atatggtaga ttttcgccat cgacaaatcg
      361 gcgagctttc cggtggacaa aagaaacgcg tctttctggc gagagcgatt gcacagcagg
      421 gtgatgtgat cctgctggat gaacctttta ccggcgttga tgtga
//
LOCUS       DAMZFT010000405          455 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.411, whole genome shotgun sequence.
ACCESSION   DAMZFT010000405 DAMZFT010000000
VERSION     DAMZFT010000405.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 455)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 455)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..455
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.411"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
BASE COUNT          132 a          105 c           96 g          122 t
ORIGIN      
        1 tcgaccgtga ctacaccact gtctgggggc agcgtgcacc actgtactac agcccgggtt
       61 acggcccagc gtcactgtac gactacaaag gcaggggccg aacctttggt ctgaactact
      121 ctgtgctgtt ctgaccggta ttcctttaca acaaaggtac gctgatatca acatggccgc
      181 tgacagccaa gttgatatca tataatacac gacataatct gtagtcaggg aggatagact
      241 ctttactgac tacagattat gtcgtgttcc gtgctcattt cctcaaaaaa atacaagaaa
      301 agaattagta ttctaacaaa aagtgaaata aattgtatca aactccctct tttaatcctg
      361 ttgagtaaat cagcttttgc aataggattg aaagagtgta agtggaatct cttccggata
      421 ctcgttacca ccgtggctag aatatctacg gctgc
//
LOCUS       DAMZFT010000406          451 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.412, whole genome shotgun sequence.
ACCESSION   DAMZFT010000406 DAMZFT010000000
VERSION     DAMZFT010000406.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 451)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 451)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..451
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.412"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..>451
                     /locus_tag="PN977_005460"
     CDS             <1..>451
                     /locus_tag="PN977_005460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000919997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="immunoglobulin-binding protein"
                     /protein_id="HDI6045335.1"
                     /translation="GDSLRGEIGGVYRDARAHTDSQVTAVRDELSRDIIAGTSAAVAY
                     TDASSLALQDEIKEKADETVQVSRAYTDKSVRDARKEAKSQAEHLSDVLVKNRAQTDA
                     AIASNTAAIRNNSHRLDLTEAWQKMATERMNNMQEQIKENRKELRESA"
BASE COUNT          146 a           83 c          137 g           85 t
ORIGIN      
        1 agggagacag tctgcgtggt gagataggcg gtgtatacag agatgcccgt gctcatactg
       61 acagtcaggt cacagcagta cgtgatgaac tcagcaggga tattattgca ggcacatcgg
      121 cagcggtggc gtatacggat gcatcttctc ttgctcttca ggatgaaata aaagaaaaag
      181 cggatgaaac agttcaggtt tcccgggctt atacagataa aagtgtacgg gatgcccgta
      241 aagaagcgaa aagccaggca gaacatctca gcgatgtgct tgtgaagaac agggcgcaga
      301 cggatgcagc aatagcctca aatacagcag cgataaggaa taacagtcat cgtctggatt
      361 tgacggaagc ctggcagaag atggcgacag agagaatgaa taatatgcag gagcagatta
      421 aagagaaccg gaaggagtta agggagagtg c
//
LOCUS       DAMZFT010000407          426 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.413, whole genome shotgun sequence.
ACCESSION   DAMZFT010000407 DAMZFT010000000
VERSION     DAMZFT010000407.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 426)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 426)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..426
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.413"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
BASE COUNT          122 a           95 c           91 g          118 t
ORIGIN      
        1 atcggtaact agctgttata ttctgaatta ttacaatagc gtcaggcctc ataattacac
       61 tgtgggttga cgccggaaga atcagagaac agataccgtt cttactgtaa aacgtggcta
      121 atattaattg agcactgcag tgttcagagc acaaagaaga aaaaagatgt gtgcgaatgc
      181 ctgtaatgtg ttaaaagaat gttccgcgtt tccggtacgc ttttcgtaac taacaatgca
      241 gggtgacgta acgctggttg tgccggaaac gcgtccctcc ccctatcttt cctgtttgga
      301 tttgccccta tatttctgga ttatccgaca aaaccggcgt gaagtattac tctcgtcgcc
      361 cagcaccata aggataacgc ccataaaatc atgaaacttt tcttccagat tggatacaat
      421 caggcc
//
LOCUS       DAMZFT010000408          424 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.414, whole genome shotgun sequence.
ACCESSION   DAMZFT010000408 DAMZFT010000000
VERSION     DAMZFT010000408.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..424
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.414"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..>424
                     /locus_tag="PN977_005461"
     CDS             <1..>424
                     /locus_tag="PN977_005461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NapC/NirT family cytochrome c"
                     /protein_id="HDI6045336.1"
                     /translation="FCVSCHSMQPVYEEYKQSVHFQNASGVRAECHDCHIPPDIPGMV
                     KRKLEASNDIYQTFIAHSIDTPEKFEAKRAELAEREWARMKENNSATCRSCHNYDAMD
                     HAKQHPEAARQMKVAAKDNQSCIDCHKGIAHQLPDMSSG"
BASE COUNT          122 a          106 c          108 g           88 t
ORIGIN      
        1 ttttgtgtca gttgccacag tatgcaaccg gtgtatgaag aatataaaca gtcggtgcat
       61 ttccagaacg cctccggcgt gcgagctgaa tgccatgact gtcatatccc gccggatatt
      121 ccaggcatgg tgaagcgcaa actggaagcg agcaacgaca tttaccagac ctttattgcc
      181 cactccattg atacaccaga gaaatttgaa gccaaacgcg cggaacttgc cgagcgtgaa
      241 tgggcgcgaa tgaaagaaaa caactcggca acctgccgct cctgccataa ctacgacgcg
      301 atggatcatg cgaagcagca tcctgaagcg gcacgtcaga tgaaggtggc agcgaaagat
      361 aatcaatcct gtatcgactg tcataaaggt attgcccacc agttaccgga tatgagtagt
      421 ggct
//
LOCUS       DAMZFT010000409          416 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.415, whole genome shotgun sequence.
ACCESSION   DAMZFT010000409 DAMZFT010000000
VERSION     DAMZFT010000409.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 416)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 416)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..416
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.415"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(<1..>416)
                     /locus_tag="PN977_005462"
     CDS             complement(<1..>416)
                     /locus_tag="PN977_005462"
                     /inference="COORDINATES: protein motif:HMM:NF012870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HDI6045337.1"
                     /translation="GIYIASESSFYRVLKSANQLKKRSRECQYKRPDVLRAVAPNQVW
                     SWDISYLPSGVRGQHFYLYMIVDIYSRKIIAAEVFASESGKHAAELLQRAIWNEKCSS
                     SNLVLHSDNGAPMRSYTLLAKMYALGVLSSYSRPRV"
BASE COUNT          118 a          100 c           88 g          110 t
ORIGIN      
        1 ctgactcttg gccgggaata agaacttagc acaccaagcg catacatttt cgccaacaac
       61 gtgtaactgc gcatgggagc accattatca gaatggagta ccagattgct ggatgaacat
      121 ttttcattcc atattgcccg ctggagtagt tctgctgcat gttttccaga ttcgctggca
      181 aatacctctg cagcaataat cttgcgactg taaatatcga cgatcatata gaggtaaaaa
      241 tgctgacctc tgaccccgga cggcaaatag ctgatatccc aactccagac ttgattcggt
      301 gcaacggccc tgagtacatc aggccgttta tactgacatt cacgggatcg tttcttcaac
      361 tggttggctg atttcagtac acgataaaag cttgattcag aagcaatata aatacc
//
LOCUS       DAMZFT010000410          402 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.416, whole genome shotgun sequence.
ACCESSION   DAMZFT010000410 DAMZFT010000000
VERSION     DAMZFT010000410.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 402)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 402)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..402
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.416"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..212
                     /locus_tag="PN977_005463"
     CDS             <1..212
                     /locus_tag="PN977_005463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IucA/IucC family siderophore biosynthesis
                     protein"
                     /protein_id="HDI6045338.1"
                     /translation="AVLSDYMKKHPQMSERFALFSLFRPQIIRVVLNPVKLTWPDLDG
                     GSRMLPNYLEDLQNPLWLVTQEYES"
     gene            209..>402
                     /locus_tag="PN977_005464"
     CDS             209..>402
                     /locus_tag="PN977_005464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SidA/IucD/PvdA family monooxygenase"
                     /protein_id="HDI6045339.1"
                     /translation="MKKSVDFIGVGTGPFNLSIAALSHQIEELDCLFFDEHPHFSWHP
                     GMLVPDCHMQTVFLKDLVSAV"
BASE COUNT           99 a           89 c           98 g          116 t
ORIGIN      
        1 cagcagtgtt gagtgattac atgaaaaaac atccacaaat gtcagagcgt tttgcgcttt
       61 tctcactttt caggccacaa atcattcgcg tggtgctgaa cccggtaaaa ctgacctggc
      121 ctgatctgga tggcggcagc cgcatgctgc cgaattacct tgaggatctg caaaatccgc
      181 tgtggctggt aactcaggaa tatgaatcat gaaaaaaagt gtcgatttta ttggtgtagg
      241 gacagggcca tttaatctca gcattgctgc gttgtcacat cagatcgaag aactggactg
      301 tctcttcttt gatgaacatc ctcatttttc ctggcatccg ggtatgctgg taccggattg
      361 tcatatgcag accgtctttc tgaaagatct ggtcagtgct gt
//
LOCUS       DAMZFT010000411          392 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.417, whole genome shotgun sequence.
ACCESSION   DAMZFT010000411 DAMZFT010000000
VERSION     DAMZFT010000411.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 392)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 392)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..392
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.417"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            45..175
                     /locus_tag="PN977_005465"
     ncRNA           45..175
                     /ncRNA_class="other"
                     /locus_tag="PN977_005465"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
BASE COUNT          108 a          119 c           89 g           76 t
ORIGIN      
        1 cttttcaaaa gtccgtgaac tctcaaacga atccgcaatt aaatattctg cccatgcggg
       61 gagggatgag aagcttcgac caaggtgcga ctcgagcgcc agcgagaaag cgttgccgca
      121 ggcaacgatc cgaagggtga agcgcgcagc gctagtaatc cttcccccac caccattact
      181 tcctgaaata ctcaaaacct gttactccga gctaattctg cccgatggca ccgcgtttac
      241 cggacctaaa atatcctccc ctgaagttcg gataagcgaa gcgcatccga caactcaccc
      301 cgcaccgtgc ccataaaaaa accccgccga agcagggtct atgtgaacta aaattcgctt
      361 agtgacgggc gcgcaccagc tgatacttac gt
//
LOCUS       DAMZFT010000412          383 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.418, whole genome shotgun sequence.
ACCESSION   DAMZFT010000412 DAMZFT010000000
VERSION     DAMZFT010000412.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 383)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 383)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..383
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.418"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            1..383
                     /locus_tag="PN977_005466"
                     /pseudo
     CDS             1..383
                     /locus_tag="PN977_005466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
BASE COUNT          101 a           94 c          105 g           83 t
ORIGIN      
        1 tgcttttccc cgattcaaaa agcggggaca gaatgatgca ttccgctacc cgcagggcgt
       61 taagctcgat caggaaaaca gccgtatttt tctgccgaaa ctgggctgga tgcgctaccg
      121 gaacagccgt caggtcacgg gtgttgtgaa aaatgtcacg gtcagccagt cctgcggtaa
      181 gtggtacatc agtattcaga cagaaagtga agtatcaact ccggttcacc cttcagcatc
      241 aatggtcggg ctggatgccg gcatggctga acttgccacg ctgtcagatg gggcacagtc
      301 tttgagcctg taaacagttt tcagaaaaac cagaagacgc tggcgagact tcagcgacag
      361 ttaagccgca aggtcaaatt cag
//
LOCUS       DAMZFT010000413          382 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.419, whole genome shotgun sequence.
ACCESSION   DAMZFT010000413 DAMZFT010000000
VERSION     DAMZFT010000413.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 382)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 382)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..382
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.419"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..>382
                     /locus_tag="PN977_005467"
     CDS             <1..>382
                     /locus_tag="PN977_005467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent siderophore receptor"
                     /protein_id="HDI6045340.1"
                     /translation="TGGLINIVSKKGQPETMKEFEAGTKSGFSSSKDHDERIAGAVSG
                     GNEHISGRLSVAYQKFGGWFDGNGDATLLDNTQTGLQYSDRLDIMGTGTLNIDESRQL
                     QLITQYYKSQGDDDYGLNLGKGFSA"
BASE COUNT          101 a           96 c          107 g           78 t
ORIGIN      
        1 gtaccggtgg cctgatcaac atcgtgagca aaaaaggcca gccggaaacc atgaaggagt
       61 ttgaggctgg caccaaaagt ggctttagca gcagtaaaga tcacgatgaa cgcattgccg
      121 gagctgtctc cggcggaaat gagcatatct ccggacgtct ttccgtggca tatcagaaat
      181 ttggcggctg gtttgacggt aacggcgatg ccaccctgct tgataacacc cagaccggcc
      241 tgcagtactc cgatcggctg gacatcatgg gaactggaac gttgaacatc gatgaatccc
      301 ggcagcttca gttgatcaca cagtactata aaagccaggg cgacgacgat tacgggctta
      361 atctcgggaa aggcttctct gc
//
LOCUS       DAMZFT010000414          379 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.420, whole genome shotgun sequence.
ACCESSION   DAMZFT010000414 DAMZFT010000000
VERSION     DAMZFT010000414.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 379)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 379)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..379
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.420"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..>379
                     /locus_tag="PN977_005468"
     CDS             <1..>379
                     /locus_tag="PN977_005468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDI6045341.1"
                     /translation="PRFKKRGQNDAFRYPQGVKLDQENSRIFLPKLGWMRYRNSRQVT
                     GIVKNVTVSQSCGKWYISIQTEREVSTPVHPSASMVGLDAGVAKLATLSDGTVFEPVN
                     SFQKNQKKLARLQRQLSRKVKFS"
BASE COUNT          106 a           90 c           97 g           86 t
ORIGIN      
        1 ttccccgatt caaaaagcgg ggacagaatg atgcattccg ctacccgcag ggtgttaagc
       61 tcgatcagga aaacagccgt atttttctgc cgaaacttgg ctggatgcgc taccggaata
      121 gccgtcaggt cacgggtatt gtgaaaaatg ttactgtcag ccagtcctgc ggtaagtggt
      181 acatcagtat tcagacagaa agagaggtat ccactcctgt tcacccttca gcatcaatgg
      241 tcggactgga tgctggcgtg gctaaactcg ccacgctgtc agatggcaca gtctttgaac
      301 ctgtaaatag ttttcagaaa aaccagaaga agctggcgag acttcagcga cagttaagcc
      361 gcaaggtcaa attcagcaa
//
LOCUS       DAMZFT010000415          365 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.421, whole genome shotgun sequence.
ACCESSION   DAMZFT010000415 DAMZFT010000000
VERSION     DAMZFT010000415.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 365)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 365)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..365
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.421"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..>365
                     /locus_tag="PN977_005469"
     CDS             <1..>365
                     /locus_tag="PN977_005469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDI6045342.1"
                     /translation="GMDNQILALYARGMTTREITSAFKEMYDADVSPTLISKVTDAVK
                     EQVSEWQNRPLDALYPIVYLDCIVVKVRHSGSVINKAVFLALGINTDGQKELLDMWLA
                     ENEGAKFWLNVLTELKNRG"
BASE COUNT           97 a           86 c          100 g           82 t
ORIGIN      
        1 cggcatggac aaccagatcc tggcgctgta tgccagaggg atgactaccc gcgaaattac
       61 ctcagccttc aaagaaatgt acgacgccga tgtctcgccc acgctgatat ccaaagtgac
      121 cgatgcggtt aaagaacagg tctctgaatg gcaaaaccga ccgttggatg cactgtatcc
      181 cattgtttat cttgactgta ttgtggttaa ggttcgtcac agcgggagtg tcattaacaa
      241 agcggtgttc ctcgcgctgg gcatcaatac cgacggtcag aaagagctgc tggatatgtg
      301 gctggccgaa aatgaaggtg caaagttctg gctgaatgta ctgacggaac tgaaaaatcg
      361 cggcc
//
LOCUS       DAMZFT010000416          357 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.422, whole genome shotgun sequence.
ACCESSION   DAMZFT010000416 DAMZFT010000000
VERSION     DAMZFT010000416.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 357)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 357)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..357
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.422"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(64..>357)
                     /locus_tag="PN977_005470"
     CDS             complement(64..>357)
                     /locus_tag="PN977_005470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001407399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion protein E"
                     /protein_id="HDI6045343.1"
                     /translation="SVCNPDQVYLRNHEGCPHCWRGVTGRTVIAEVISPDARFFQLYR
                     EKGRIEARTYWHRELGGMTRNQHLLGKINSGQVDPLAAHYISPIDEDSYTLLH"
BASE COUNT           69 a          101 c           97 g           90 t
ORIGIN      
        1 atccagacag tcagtgctgt cagctcaatg tcggtgttgt tgagtttttc cagaaatctc
       61 atgtcagtgc agcagcgtat agctgtcctc gtcgatggga gagatgtaat gggccgccag
      121 cggatcaacc tgcccgctgt tgatttttcc cagcagatgc tgatttcgtg tcatgccgcc
      181 cagctcccgg tgccagtagg ttctggcctc aatacgcccc ttttcccggt acaactggaa
      241 aaaacgggca tccggggaga tgacttctgc gatcaccgtt cgtcctgtca cgccacgcca
      301 gcagtgcggg cagccttcat gatttcgcag atatacctgg tccgggttgc agacgct
//
LOCUS       DAMZFT010000417          357 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.423, whole genome shotgun sequence.
ACCESSION   DAMZFT010000417 DAMZFT010000000
VERSION     DAMZFT010000417.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 357)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 357)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..357
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.423"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(64..>357)
                     /locus_tag="PN977_005471"
     CDS             complement(64..>357)
                     /locus_tag="PN977_005471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001407399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion protein E"
                     /protein_id="HDI6045344.1"
                     /translation="SVCNPDQVYLRNHEGCPHCWRGVTGRTVIAEVISPDARFFQLYR
                     EKGRIEARTYWHRELGGMTRNQHLLGKINSGQVDPLAAHYISPVDEDSYTLLH"
BASE COUNT           72 a           99 c           93 g           93 t
ORIGIN      
        1 atccagatgg tcagcgctgt cagctcaatg tcggtgctgt tgagtttttc cagaaatttc
       61 atatcagtgc agcagcgtat agctgtcctc atcgacggga gatatataat gggccgccag
      121 cggatcaacc tgtccgctgt tgatttttcc cagcagatgc tgattacggg tcattccgcc
      181 cagttcccgg tgccagtatg ttctggcctc aatacgcccc ttttcccggt acaactggaa
      241 aaaacgggca tccggggaga tgacttctgc gatcactgtt cgtcctgtca cgccacgcca
      301 gcagtgcggg cagccttcat gatttcgcag atatacctgg tccgggttgc agacgct
//
LOCUS       DAMZFT010000418          355 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.424, whole genome shotgun sequence.
ACCESSION   DAMZFT010000418 DAMZFT010000000
VERSION     DAMZFT010000418.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..355
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.424"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..>355
                     /locus_tag="PN977_005472"
     CDS             <1..>355
                     /locus_tag="PN977_005472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HDI6045345.1"
                     /translation="VITHGTDTMEETAYFLDLTVKCDKPVVMVGAMRPSTSMSADGPF
                     NLYNAVVTAADKASANRGVLVVMNDTVLDGRDVTKTNTTNVATFKSVNYGPLGYIHNG
                     KIDYQRTPARKHTSDT"
BASE COUNT           92 a          103 c           85 g           75 t
ORIGIN      
        1 tcgtcattac ccacggtacc gacacgatgg aagaaaccgc ttacttcctt gacctgacgg
       61 tgaaatgtga caaaccggtg gtgatggtcg gtgcaatgcg cccgtccacg tccatgagcg
      121 cagacggtcc cttcaatctg tataacgcgg tagttaccgc agctgataaa gcctccgcta
      181 atcgtggcgt actggtagta atgaacgaca ctgttctgga tggccgtgac gtcactaaaa
      241 ccaacaccac caacgtagcg accttcaagt ctgttaacta cggccctctg ggttacattc
      301 acaacggtaa gattgactac cagcgcaccc cggcacgtaa gcacaccagt gatac
//
LOCUS       DAMZFT010000419          341 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.425, whole genome shotgun sequence.
ACCESSION   DAMZFT010000419 DAMZFT010000000
VERSION     DAMZFT010000419.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 341)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 341)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..341
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.425"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(<1..178)
                     /locus_tag="PN977_005473"
     CDS             complement(<1..178)
                     /locus_tag="PN977_005473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDI6045346.1"
                     /translation="MKRLQAFKFQLRPGGQQEHEMRRFAGACRFVFNRALALQNENHE
                     AGNKYIPYGKMASWL"
     gene            186..341
                     /locus_tag="PN977_005474"
                     /pseudo
     CDS             186..341
                     /locus_tag="PN977_005474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313209.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT           91 a           82 c           72 g           96 t
ORIGIN      
        1 ccagccagga agccattttc ccgtaaggga tgtatttatt accggcctca tgattctcat
       61 tctgaagcgc cagagcacga ttgaaaacga aacgacatgc gcctgcgaag cgcctcattt
      121 catgctcctg ttgaccaccg ggtcttaact ggaatttaaa tgcctgtagt cgtttcacag
      181 gtcaaattat attttggtct gtgaaaaaag aaaccgatat ccggcgtgga aggcattgtg
      241 ttttcctgat gcatgttcac ctggtctttg tcccaaatac cgacgccaga tttttgacca
      301 tgacgcgaca gaaaaactac gcacttactt ttcaaatgta t
//
LOCUS       DAMZFT010000420          338 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.426, whole genome shotgun sequence.
ACCESSION   DAMZFT010000420 DAMZFT010000000
VERSION     DAMZFT010000420.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 338)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 338)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..338
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.426"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..>338
                     /locus_tag="PN977_005475"
     CDS             <1..>338
                     /locus_tag="PN977_005475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-dependent hydrogenase large subunit"
                     /protein_id="HDI6045347.1"
                     /translation="WAFVERICGVCTGVHALASVYAIEDAIGIKVPDNANIIRNIMLA
                     TLWCHDHLVHFYQLAGMDWIDVLDALKADPRKTSELAQSLSSWPKSSPGYFFDVQNRL
                     KKFVEGGQLG"
BASE COUNT           75 a           92 c           91 g           80 t
ORIGIN      
        1 tgggcgttcg ttgaacgtat ctgcggcgtc tgtactggcg tacacgccct ggcttcggtt
       61 tacgccatcg aagatgccat cggtattaaa gtgccggaca acgccaatat catccgcaac
      121 attatgctgg caacgctctg gtgccacgat catctggtgc acttctatca gcttgccggg
      181 atggattgga tcgatgtgtt agatgcgctg aaagccgacc cgcggaaaac ctccgaactg
      241 gcgcaaagcc tctcctcatg gccgaaatca tcccctggct atttcttcga cgtacaaaac
      301 cgcctgaaga aattcgttga aggcgggcag ttggggat
//
LOCUS       DAMZFT010000421          333 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.427, whole genome shotgun sequence.
ACCESSION   DAMZFT010000421 DAMZFT010000000
VERSION     DAMZFT010000421.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 333)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 333)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..333
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.427"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            47..196
                     /locus_tag="PN977_005476"
                     /pseudo
     CDS             47..196
                     /locus_tag="PN977_005476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708774.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT           94 a           79 c           89 g           71 t
ORIGIN      
        1 gaacagtaaa taacattact ctgggtaagc atccggaggc gaaacaatga aactggccct
       61 gacgctggaa gccgacagcg ttaacgtgca ggcgctgaac atggggcgaa ttgtcgttga
      121 cgtcgatggt gttaatctca ttgaactgat taacaaggtc gctgaaaacg gttattcact
      181 ccgcgtggtg gatgaacgtg acgctaccga aacaccggcg acttctgcca gccctcatca
      241 gttgccggaa gacaatacag tgatacagag cgtgatgcca ttaaccactg aaacagcgct
      301 ggatatcctc attgcgtggc tgcaggacaa tat
//
LOCUS       DAMZFT010000422          330 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.428, whole genome shotgun sequence.
ACCESSION   DAMZFT010000422 DAMZFT010000000
VERSION     DAMZFT010000422.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 330)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 330)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..330
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.428"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            1..330
                     /locus_tag="PN977_005477"
                     /pseudo
     CDS             1..330
                     /locus_tag="PN977_005477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612262.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT           88 a           91 c           87 g           64 t
ORIGIN      
        1 acgtcagctc aggcgagagc gccaaggtat tgcgccctct gcaacaccca ttacccctga
       61 acagcaacgt attcgcgagc tggaaaagca ggttcgccga ctggaggaac aaaatacgat
      121 attaaaaaag gctaccgcgc tcttgatgtc cgactcactg aacggtttac gatagtttcc
      181 agactgagtg acagccactc agttgtcagc ctctgttccg cactgggaat acaccgcagc
      241 agttaccggt actggcgaaa acgacgcgat acggttaatt cggctcgcgt caggttgtgc
      301 agcgaaatac gccgggcatg gaaccagagc
//
LOCUS       DAMZFT010000423          329 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.429, whole genome shotgun sequence.
ACCESSION   DAMZFT010000423 DAMZFT010000000
VERSION     DAMZFT010000423.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 329)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 329)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..329
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.429"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            52..>329
                     /locus_tag="PN977_005478"
     CDS             52..>329
                     /locus_tag="PN977_005478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001383442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HDI6045348.1"
                     /translation="MVVVQNQPDEIPVLEWQGVRVVTTETLARGYGVDEANIRNNLSR
                     NLDRFEEGKHYFLLTGLKLREFKNRVTGSYSVGKNARSLTLWTERGAAR"
BASE COUNT           99 a           66 c           81 g           83 t
ORIGIN      
        1 actaacgtta aatcagaaaa aatttatgca ccttcagttc aggaatcgga actggtggtt
       61 gttcagaatc agccggatga aattcccgtt ctggaatggc agggagtacg tgtcgtgaca
      121 accgaaactc ttgctagagg gtatggggtc gatgaagcca atattcgcaa caatttgtct
      181 cgcaaccttg accgctttga agaaggtaag cattactttc ttctaactgg tttaaaatta
      241 agagaattta agaacagagt aaccggaagt tactctgttg gtaagaacgc cagaagcctt
      301 acactctgga cagagcgcgg cgctgcacg
//
LOCUS       DAMZFT010000424          327 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.430, whole genome shotgun sequence.
ACCESSION   DAMZFT010000424 DAMZFT010000000
VERSION     DAMZFT010000424.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 327)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 327)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..327
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.430"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(1..327)
                     /locus_tag="PN977_005479"
                     /pseudo
     CDS             complement(1..327)
                     /locus_tag="PN977_005479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001368704.1"
                     /note="internal stop; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="ISL3 family transposase"
BASE COUNT           86 a           88 c           83 g           70 t
ORIGIN      
        1 gattgactat taacataccg cagcagtggc cccgatgcca cgcccactca tcaataccga
       61 catgaggcgc gcctgactgt ttcgtctccg gggtattgat aagcctgcga agaagagtat
      121 cagcactgca acggagaccc actgccgttg cagcccgttt tccagcctca cctccagcta
      181 ttaatcccag ttgacattgt taattttgta acgcctgtga agactgctgg tgtgaactgg
      241 cgaagggagc aagcgactcg gcaaaaattt tacgtgaaca agccgggtta cggcagtacc
      301 agtggcggac ggaaaataca agccata
//
LOCUS       DAMZFT010000425          323 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.431, whole genome shotgun sequence.
ACCESSION   DAMZFT010000425 DAMZFT010000000
VERSION     DAMZFT010000425.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 323)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 323)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..323
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.431"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(<1..>323)
                     /locus_tag="PN977_005480"
     rRNA            complement(<1..>323)
                     /locus_tag="PN977_005480"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT           62 a          108 c           69 g           84 t
ORIGIN      
        1 cttttcctgg aagcagggca tttgttgctt cagcaccgta gtgcctcgtc atcacgcctc
       61 agccttgatt ttccggattt gcctggaaaa ccagcctaca cgcttaaacc gggacaaccg
      121 tcgcccggcc aacatagcct tctccgtccc cccttcgcag taacaccaag tacaggaata
      181 ttaacctgtt tcccatcgac tacgcctttc ggcctcgcct taggggtcga ctcaccctgc
      241 cccgattaac gttggacagg aacccttggt cttccggcga gcgggctttt cacccgcttt
      301 atcgttactt atgtcagcat tcg
//
LOCUS       DAMZFT010000426          314 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.432, whole genome shotgun sequence.
ACCESSION   DAMZFT010000426 DAMZFT010000000
VERSION     DAMZFT010000426.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 314)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 314)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..314
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.432"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            21..>314
                     /locus_tag="PN977_005481"
     CDS             21..>314
                     /locus_tag="PN977_005481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS26 family transposase"
                     /protein_id="HDI6045349.1"
                     /translation="MNPFKGRHFQRDIILWAVRWYCKYGISYRELQEMLAERGVNVDH
                     STIYRWVQRYAPEMEKRLRWYWRNPSDLCPWHMDETYVKVNGRWAYLYRAVDSR"
BASE COUNT           67 a           81 c           93 g           73 t
ORIGIN      
        1 ttttgctgat ggagctgcac atgaacccat tcaaaggccg gcattttcag cgtgacatca
       61 ttctgtgggc cgtacgctgg tactgcaaat acggcatcag ttaccgtgag ctgcaggaga
      121 tgctggctga acgcggagtg aatgtcgatc actccacgat ttaccgctgg gttcagcgtt
      181 atgcgcctga aatggaaaaa cggctgcgct ggtactggcg taacccttcc gatctttgcc
      241 cgtggcacat ggatgaaacc tacgtgaagg tcaatggccg ctgggcgtat ctgtaccggg
      301 ccgtcgacag ccgg
//
LOCUS       DAMZFT010000427          312 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.433, whole genome shotgun sequence.
ACCESSION   DAMZFT010000427 DAMZFT010000000
VERSION     DAMZFT010000427.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 312)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 312)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..312
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.433"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..>312
                     /locus_tag="PN977_005482"
     CDS             <1..>312
                     /locus_tag="PN977_005482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HDI6045350.1"
                     /translation="KLMDLSINKNWIDKEEYPQSAAIDLRCVNMVADLWHAPAPKNGQ
                     AVGTNTIGSSEACMLGGMAMKWRWRKRMEAAGKPTDKPNLVCGPVQICWHKFARYWDV
                     EL"
BASE COUNT           80 a           74 c           90 g           68 t
ORIGIN      
        1 aagttgatgg atttatccat taacaaaaac tggatcgaca aagaagaata tccgcaatcc
       61 gcagccatcg acctgcgttg cgtaaacatg gttgccgatc tgtggcatgc gcctgcgccg
      121 aaaaatggtc aggccgttgg caccaacacc attggttctt ccgaggcctg tatgcttggc
      181 gggatggcga tgaaatggcg ttggcgcaag cgtatggaag ctgcaggcaa accaacggat
      241 aaaccaaacc tggtgtgcgg tccggtgcaa atctgctggc ataaattcgc ccgctactgg
      301 gatgtggagt tg
//
LOCUS       DAMZFT010000428          301 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.434, whole genome shotgun sequence.
ACCESSION   DAMZFT010000428 DAMZFT010000000
VERSION     DAMZFT010000428.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 301)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 301)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..301
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.434"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
BASE COUNT           91 a           79 c           75 g           56 t
ORIGIN      
        1 aaccgtgcgc aaaatgttgt ggataagcag gacacagcag caatccacag caggcataca
       61 accgcacacc gaggttactc cgttctacag gttacgacga catgtcaata cttgcccttg
      121 acaggcattg atggaatcgt agtctcacgc tgatagtctg atcgacaata caagtgggac
      181 cgtggtccca gaccgataat cagaccgaca acacgagtgg gatcgtggtc ccagactaat
      241 aatcagaccg acgatacgag tgggaccgtg gtcccagact aataatcaga ccgacgatac
      301 g
//
LOCUS       DAMZFT010000429          300 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.435, whole genome shotgun sequence.
ACCESSION   DAMZFT010000429 DAMZFT010000000
VERSION     DAMZFT010000429.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 300)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 300)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..300
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.435"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..>300
                     /locus_tag="PN977_005483"
     CDS             <1..>300
                     /locus_tag="PN977_005483"
                     /inference="COORDINATES: protein motif:HMM:NF024776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDI6045351.1"
                     /translation="YIKGKVQCPLGQQRGRLPPLLERHKIISLVREAVANGARKGQAC
                     RCIGISIRTLQRWTNGNIITTDRRSDTVNSSPRNKLSEAERLRILELCNSPEFAS"
BASE COUNT           79 a           76 c           74 g           71 t
ORIGIN      
        1 ttatattaag ggaaaagttc aatgccctct gggacaacag cgaggaagac tgccccctct
       61 cctggagcgg cataaaatta tttctcttgt ccgtgaggcc gtcgccaacg gcgcccggaa
      121 aggtcaggct tgtaggtgta tcggtatttc tattcggaca cttcagcgct ggactaatgg
      181 caacatcatc accacggacc gaagatccga tactgtaaac agctctcccc gaaataagct
      241 gtcagaagcg gaaagattgc gaatactgga gttgtgcaac tctccagagt ttgcatcctt
//
LOCUS       DAMZFT010000430          294 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.436, whole genome shotgun sequence.
ACCESSION   DAMZFT010000430 DAMZFT010000000
VERSION     DAMZFT010000430.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 294)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 294)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..294
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.436"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(20..244)
                     /locus_tag="PN977_005484"
     CDS             complement(20..244)
                     /locus_tag="PN977_005484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001705644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Derepression protein"
                     /protein_id="HDI6045352.1"
                     /translation="MEAYHKLNRASAVSQFVGGDLIHRELSGLHQLYIPHIFSYLNED
                     IDFVLNELKAKGLCRDFLAQQKDRGDRTHV"
BASE COUNT           70 a           73 c           85 g           66 t
ORIGIN      
        1 ctcaccgggc tggggaaaat caaacatgcg tcctgtctcc ccggtctttc tgctgggcga
       61 gaaaatcgcg gcacaggcct ttggctttca gctcattcag cacaaaatca atatcttcat
      121 tcaggtagct gaaaatatgc ggaatgtaga gctgatgcag gccggagagt tcacggtgaa
      181 tcaaatcacc cccaacaaac tgggatacgg cgctggcgcg gttgagctta tggtaagcct
      241 caatgctgag gtgttcacgg gcgtcatgac gcgctgagac ggtctgaggg gctt
//
LOCUS       DAMZFT010000431          286 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.437, whole genome shotgun sequence.
ACCESSION   DAMZFT010000431 DAMZFT010000000
VERSION     DAMZFT010000431.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 286)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 286)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..286
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.437"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..>286
                     /locus_tag="PN977_005485"
     CDS             <1..>286
                     /locus_tag="PN977_005485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HDI6045353.1"
                     /translation="HAPAFWRWLDRAAGGRAEKRDVILAALFMVLANRYDWQLFLEVT
                     GPGGSGKSIMAEIATLLAGEDNATSATIETLESPRERAALTGFSLIRLPD"
BASE COUNT           52 a           91 c           88 g           55 t
ORIGIN      
        1 cccacgctcc cgcgttctgg cgctggcttg accgtgctgc cggtggtcgt gcggaaaaac
       61 gcgacgtaat tctggccgca ctgtttatgg tgctggcaaa ccgctacgac tggcagctct
      121 ttctggaggt gaccggtccc ggcggcagcg gcaaaagtat catggccgaa atagccaccc
      181 tgctggccgg ggaggataac gccacgtcgg ccaccattga gacgctggaa tccccgcgtg
      241 aacgtgccgc gttaactggc ttctcactga tacgcctgcc ggacca
//
LOCUS       DAMZFT010000432          277 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.438, whole genome shotgun sequence.
ACCESSION   DAMZFT010000432 DAMZFT010000000
VERSION     DAMZFT010000432.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 277)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 277)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..277
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.438"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..>277
                     /locus_tag="PN977_005486"
     CDS             <1..>277
                     /locus_tag="PN977_005486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001260625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HDI6045354.1"
                     /translation="APSVRESELVVVQNQSDEIPVLEWLGVRVVTTETLARGYSTDEA
                     NIRKNLSRNSDRFEEGKHYYLLTGSKLKEFKRLVTISHLVSKYTSQV"
BASE COUNT           81 a           51 c           67 g           78 t
ORIGIN      
        1 atgcaccttc agttcgggaa tcggagctgg tggttgttca gaatcagtct gatgaaattc
       61 ccgttctgga atggctggga gtgcgtgtcg taacgaccga aactcttgct agagggtata
      121 gtaccgatga agccaatatt cgtaaaaatt tgtctcgcaa ctccgatcgc tttgaagaag
      181 gcaagcatta ctatctctta accggttcaa agttaaagga atttaaaagg ctagtgacta
      241 ttagtcactt ggttagcaaa tatacaagtc aggtaat
//
LOCUS       DAMZFT010000433          277 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.439, whole genome shotgun sequence.
ACCESSION   DAMZFT010000433 DAMZFT010000000
VERSION     DAMZFT010000433.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 277)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 277)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..277
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.439"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(116..>277)
                     /locus_tag="PN977_005487"
     CDS             complement(116..>277)
                     /locus_tag="PN977_005487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Polarity suppression protein"
                     /protein_id="HDI6045355.1"
                     /translation="EKINYSAQDSDILTTIGFRPDAASVDDSREKFTPAQNMIFSRKS
                     AQLASHQSV"
BASE COUNT           71 a           60 c           72 g           74 t
ORIGIN      
        1 ccgtgccggg aatggcgcgg tatacagaaa atgaggcagg aaaacgcatg caaagccatg
       61 caccttatcg atgcatggct tttttcagta aaatcggacg gattttcggg gagttttaca
      121 cagactgatg tgatgccagt tgcgcacttt tacgcgaaaa aatcatgttc tgcgcagggg
      181 tgaatttttc acggctgtca tccaccgaag ccgcgtcagg cctgaatccg atggtcgtta
      241 aaatgtcgct atcctgtgcg gaataattaa ttttttc
//
LOCUS       DAMZFT010000434          276 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.440, whole genome shotgun sequence.
ACCESSION   DAMZFT010000434 DAMZFT010000000
VERSION     DAMZFT010000434.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 276)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 276)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..276
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.440"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
BASE COUNT           53 a           82 c           79 g           62 t
ORIGIN      
        1 actgcaccgc tggccagtcg tttaatctca gcgatctgtg ccggttcacc agaaaaatac
       61 atacgattcg agcaccagtt tggcatggga attttcctct cacttctgct tcagcagaga
      121 gcaccgggag ggcgcccctg tagcgcccgt tccccgctgc ctgtgggctg ttcaccgcgt
      181 ccggtaacgg gtcaggccga ccgtggaata ccgcagccgt cgtctttttg acggcaaggt
      241 tatgccgcga aagcggcttg aagggcgttc atcaga
//
LOCUS       DAMZFT010000435          276 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.441, whole genome shotgun sequence.
ACCESSION   DAMZFT010000435 DAMZFT010000000
VERSION     DAMZFT010000435.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 276)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 276)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..276
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.441"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..>276
                     /locus_tag="PN977_005488"
     CDS             <1..>276
                     /locus_tag="PN977_005488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000783678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HDI6045356.1"
                     /translation="VLKNRHQPCPVCGGSDRFRFDDREGRGTWYCNQCGAGDGLKLVE
                     KVFGVSPSDAAAKVAAVTGSLPPADPAVTAAAGAETDAARKNAAALAQ"
BASE COUNT           49 a           82 c           96 g           49 t
ORIGIN      
        1 gtgctgaaaa accgtcatca gccctgtccg gtctgtggcg ggagtgaccg cttccgtttt
       61 gatgacaggg aggggcgcgg cacctggtac tgcaatcagt gtggtgccgg tgacggcctg
      121 aaactggttg aaaaggtgtt tggtgtttcc ccgtccgatg cggccgcaaa ggtggctgcc
      181 gtgaccggaa gcctgccccc ggctgacccg gcagtgacgg ccgccgccgg tgctgaaaca
      241 gacgctgccc ggaagaacgc cgccgcactg gcacaa
//
LOCUS       DAMZFT010000436          273 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.denovo.442, whole genome shotgun sequence.
ACCESSION   DAMZFT010000436 DAMZFT010000000
VERSION     DAMZFT010000436.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 273)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 273)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..273
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.denovo.442"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..>273
                     /locus_tag="PN977_005489"
     CDS             <1..>273
                     /locus_tag="PN977_005489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB-like nuclease domain-containing protein"
                     /protein_id="HDI6045357.1"
                     /translation="DEVVANDYNPNVMSSSEKKLLKHSLEQDGFTQPVVVSEEKEHYL
                     VVDGFHRQLLGRKADTRKRLKGWLPVACINPERKGQASRIAATIRHN"
BASE COUNT           77 a           55 c           80 g           61 t
ORIGIN      
        1 gatgaggtgg tggccaacga ttacaatccc aatgtcatgt cttccagtga aaaaaaactg
       61 cttaaacatt cactggagca ggatggattc acgcagcctg tggtggtttc ggaagagaag
      121 gagcattatc tggtcgtgga tggttttcat cgtcagttgc tgggcaggaa agcggatacg
      181 agaaaacgtc tgaagggctg gctgccagtg gcctgtatta atccagagag aaagggacag
      241 gcatcacgta ttgccgcaac catacggcat aac
//
LOCUS       DAMZFT010000437         3001 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.guided.282, whole genome shotgun sequence.
ACCESSION   DAMZFT010000437 DAMZFT010000000
VERSION     DAMZFT010000437.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3001)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3001)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3001
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.guided.282"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(<1..210)
                     /locus_tag="PN977_005490"
     CDS             complement(<1..210)
                     /locus_tag="PN977_005490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308291.1"
                     /note="catalyzes the hydrolysis of Xaa-His dipeptides;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HDI6045358.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRKPATAGMENRKP"
     gene            471..929
                     /gene="gpt"
                     /locus_tag="PN977_005491"
     CDS             471..929
                     /gene="gpt"
                     /locus_tag="PN977_005491"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HDI6045359.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            1021..2265
                     /gene="frsA"
                     /locus_tag="PN977_005492"
     CDS             1021..2265
                     /gene="frsA"
                     /locus_tag="PN977_005492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HDI6045360.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     TQAMVWQQKACAEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDEA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITDWIEKRLC"
     gene            2323..2724
                     /gene="crl"
                     /locus_tag="PN977_005493"
     CDS             2323..2724
                     /gene="crl"
                     /locus_tag="PN977_005493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HDI6045361.1"
                     /translation="MTLPSGHPKSRLIKKFTALGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWKNVPVKDAEVVERLEHTLRE
                     FHEKLRELLTTLNLKLEPADDFRDEPVKLTA"
     gene            complement(2763..>3001)
                     /locus_tag="PN977_005494"
     CDS             complement(2763..>3001)
                     /locus_tag="PN977_005494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="porin"
                     /protein_id="HDI6045362.1"
                     /translation="YQFDFGLRPSLGYVLSKGKDIEGIGDEDLVNYIDVGATYYFNKN
                     MSAFVDYKINQLDSDNKLNINNDDIVAVGMTYQF"
BASE COUNT          750 a          719 c          773 g          759 t
ORIGIN      
        1 cggtttacga ttttccatac ctgcggtggc aggtttacga atcaggatat tacccacctg
       61 atcgcgttcg acatggaaac ctttctcttt tgcccaacca acaatgtatt cagcgagttg
      121 ctcttcatga taggacgggt gaggaataga acagattttg gcaaaaatat cccacagcgg
      181 ctgtggagat aattgagaca gttcagacac gttaagtctc cttgtcgatc acccgcaaaa
      241 cagtattgca ggtcacaggg ttagcagaaa atgttgtcaa cacaagacag gcttgcgaga
      301 tatgtttgag aataccactt tatcccgcgt cagggagagg cagtgcgtaa aaagacgcgg
      361 actcatgtga aatactggtt tttagtgcgc cagatctcta taatctcgcg caacctattt
      421 tcccctcgaa cactttttaa gccgtagata aacaggctgg gacacttcac atgagcgaaa
      481 aatacatcgt cacctgggac atgttgcaga tccatgcacg taaactcgca agccgactga
      541 tgccttctga acaatggaaa ggcattattg ccgtaagccg tggcggtctg gtaccgggtg
      601 cgttactggc gcgtgaactg ggtattcgtc atgtcgatac cgtttgtatt tccagctacg
      661 atcacgacaa ccagcgcgag cttaaagtgc tgaaacgcgc agaaggcgat ggcgaaggct
      721 tcatcgttat tgatgacctg gtggataccg gtggtactgc ggttgcgatt cgtgaaatgt
      781 atccaaaagc gcactttgtc accatcttcg caaaaccggc tggtcgtccg ctggttgatg
      841 actatgttgt tgatatcccg caagatacct ggattgaaca gccgtgggat atgggcgtcg
      901 tattcgtccc gccaatctcc ggtcgctaat cttttcaacg cctggcactg ccgggcgttg
      961 ttctttttaa cttcaggcgg gttacaatag tttccagtaa gtattctgga ggctgcatcc
     1021 atgacacagg caaacctgag cgaaaccctg ttcaaacccc gctttaaaca tcctgaaacc
     1081 tcgacgctag tccgccgctt taatcacggc gcacaaccgc ctgtgcagtc ggcccttgat
     1141 ggtaaaacca tccctcactg gtatcgcatg attaaccgtc tgatgtggat ctggcgcggc
     1201 attgacccac gtgaaatcct cgacgtccag gcgcgtattg tgatgagcga tgccgaacgt
     1261 accgacgatg atttatacga tacggtgatt ggctaccgcg gcggcaactg gatttatgag
     1321 tgggctaccc aggcgatggt gtggcaacaa aaagcctgtg cggaagaaga tccgcaactc
     1381 agcggtcgac actggctgca tgcggctacg ttgtacaaca ttgctgccta tcctcatctg
     1441 aaaggagatg acctggccga gcaagcgcag gctttgtcaa accgcgccta tgaagaggcc
     1501 gctcagcgtc tgccgggcac gatgcggcag atggagttta ccgtacccgg cggtgcgccc
     1561 atcaccggct ttttgcatat gccgaaaggt gatggcccgt tcccgacagt attaatgtgt
     1621 ggtggtctgg atgcgatgca gacggactat tacagcctgt atgaacgtta ttttgcgccg
     1681 cgcggcattg cgatgctgac tattgatatg ccgtcggtgg gcttttcttc aaaatggaag
     1741 ctcacccagg actccagcct gttgcatcag cacgtcttaa aggcgctgcc taacgtaccg
     1801 tgggtagatc acactcgcgt cgcggccttt ggtttccgtt tcggcgctaa cgttgccgtg
     1861 cgtctggcat accttgaatc gccgcgtctg aaagcggttg cctgtcttgg tccggtagtt
     1921 cataccctgt tgagtgattt taagtgccag caacaggtgc cggaaatgta tcttgacgtt
     1981 ctggcgagcc gtttggggat gcatgatgct tccgacgaag cgttgcgcgt ggagctgaat
     2041 cgctattcat taaaagtgca aggattgctg ggacgtcgct gcccaacgcc aatgttatca
     2101 ggctactgga agaacgatcc gttcagcccg gaagaggact cacgcttaat cacctcatca
     2161 tctgctgacg gtaaattgtt agagatccca tttaacccgg tgtatcggaa ttttgacaaa
     2221 ggtcttcagg aaatcaccga ctggatcgaa aaacgattgt gttaaaaatt tgctaaattt
     2281 taccaatttg gtaaaacagt tgcatcacaa caggagattg caatgacgtt accgagtgga
     2341 cacccgaaga gcagattgat caaaaaattt accgcactag gcccgtatat tcgtgaaggt
     2401 aagtgcgaag ataatcgatt ctttttcgat tgtctggctg tatgcgtcaa cgtgaaaccg
     2461 gcaccggaag tgcgtgagtt ctggggctgg tggatggaac tggaagcgca ggaatcccgt
     2521 tttacctaca gttaccagtt tggtctgttc gataaagcag gcgactggaa gaacgttccg
     2581 gtaaaagatg ccgaagtggt tgagcgactg gaacataccc tgcgtgagtt tcatgagaag
     2641 ctgcgtgaac tgctgacgac actgaatctg aagctggaac cggcggatga ttttcgtgat
     2701 gagccggtga agttaacggc gtgagtgaaa tgtgccggat gcatcacatc cggcaatatc
     2761 cattaaaact gataggtcat gccaaccgcg acaatatcat cattattgat gttcagctta
     2821 ttatcgctat ccagttggtt tattttataa tcaacaaatg ccgacatatt tttgttgaaa
     2881 taatacgtag caccaacatc gatataattg accagatctt catcaccgat gccttcaata
     2941 tctttccctt tcgataagac ataacccagc gacggacgca gaccaaagtc aaactggtat
     3001 t
//
LOCUS       DAMZFT010000438         2606 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.guided.294, whole genome shotgun sequence.
ACCESSION   DAMZFT010000438 DAMZFT010000000
VERSION     DAMZFT010000438.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2606)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2606)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2606
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.guided.294"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(<1..479)
                     /gene="tetR(B)"
                     /locus_tag="PN977_005495"
     CDS             complement(<1..479)
                     /gene="tetR(B)"
                     /locus_tag="PN977_005495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000088605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance transcriptional
                     repressor TetR(B)"
                     /protein_id="HDI6045363.1"
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWH
                     VKNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLG
                     TRPTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERET
                     "
     gene            561..1766
                     /gene="tet(B)"
                     /locus_tag="PN977_005496"
     CDS             561..1766
                     /gene="tet(B)"
                     /locus_tag="PN977_005496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001089064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(B)"
                     /protein_id="HDI6045364.1"
                     /translation="MNSSTKIALVITLLDAMGIGLIMPVLPTLLREFIASEDIANHFG
                     VLLALYALMQVIFAPWLGKMSDRFGRRPVLLLSLIGASLDYLLLAFSSALWMLYLGRL
                     LSGITGATGAVAASVIADTTSASQRVKWFGWLGASFGLGLIAGPIIGGFAGEISPHSP
                     FFIAALLNIVAFLVVMFWFRETKNTRDNTDTEVGVETQSNSVYITLFKTMPILLIIYF
                     SAQLIGQIPATVWVLFTENRFGWNSMMVGFSLAGLGLLHSVFQAFVAGRIATKWGEKT
                     AVLLGFIADSSAFAFLAFISEGWLVFPVLILLAGGGIALPALQGVMSIQTKSHQQGAL
                     QGLLVSLTNATGVIGPLLFAVIYNHSLPIWDGWIWIIGLAFYCIIILLSMTFMLTPQA
                     QGSKQETSA"
     gene            complement(1879..2472)
                     /gene="tetC"
                     /locus_tag="PN977_005497"
     CDS             complement(1879..2472)
                     /gene="tetC"
                     /locus_tag="PN977_005497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000428546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracyline resistance-associated
                     transcriptional repressor TetC"
                     /protein_id="HDI6045365.1"
                     /translation="MENKNHQQENFKSTYQSLVNSARILFVEKGYQAVSIDEISGKAL
                     VTKGAFYHHFKNKKQLLSACYKQQLIMIDAYITTKTDLTNGWSALESIFEHYLDYIID
                     NNKNLIPIQEVMPIIGWNELEKISLEYITGKVNAIVSKLIQENQLKAYDDDVLKNLLN
                     GWFMHIAIHAKNLKELADKKGQFIAIYRGFLLSLKDK"
BASE COUNT          689 a          485 c          527 g          905 t
ORIGIN      
        1 gtttcccttt cttctttagc gacttgatgc tcttgatctt ccaatacgca acctaaagta
       61 aaatgcccca cagcgctgag tgcatataat gcattctcta gtgaaaaacc ttgttggcat
      121 aaaaaggcta attgattttc gagagtttca tactgttttt ctgtaggccg tgtacctaaa
      181 tgtacttttg ctccatcgcg atgacttagt aaagcacatc taaaactttt agcgttatta
      241 cgtaaaaaat cttgccagct ttccccttct aaagggcaaa agtgagtatg gtgcctatct
      301 aacatctcaa tggctaaggc gtcgagcaaa gcccgcttat tttttacatg ccaatacaat
      361 gtaggctgct ctacacctag cttctgggcg agtttacggg ttgttaaacc ttcgattccg
      421 acctcattaa gcagctctaa tgcgctgtta atcactttac ttttatctaa tctagacatc
      481 attaattcct aatttttgtt gacactctat cattgataga gttattttac cactccctat
      541 cagtgataga gaaaagtgaa atgaatagtt cgacaaagat cgcattggta attacgttac
      601 tcgatgccat ggggattggc cttatcatgc cagtcttgcc aacgttatta cgtgaattta
      661 ttgcttcgga agatatcgct aaccactttg gcgtattgct tgcactttat gcgttaatgc
      721 aggttatctt tgctccttgg cttggaaaaa tgtctgaccg atttggtcgg cgcccagtgc
      781 tgttgttgtc attaataggc gcatcgctgg attacttatt gctggctttt tcaagtgcgc
      841 tttggatgct gtatttaggc cgtttgcttt cagggatcac aggagctact ggggctgtcg
      901 cggcatcggt cattgccgat accacctcag cttctcaacg cgtgaagtgg ttcggttggt
      961 taggggcaag ttttgggctt ggtttaatag cggggcctat tattggtggt tttgcaggag
     1021 agatttcacc gcatagtccc ttttttatcg ctgcgttgct aaatattgtc gctttccttg
     1081 tggttatgtt ttggttccgt gaaaccaaaa atacacgtga taatacagat accgaagtag
     1141 gggttgagac gcaatcgaat tcggtataca tcactttatt taaaacgatg cccattttgt
     1201 tgattattta tttttcagcg caattgatag gccaaattcc cgcaacggtg tgggtgctat
     1261 ttaccgaaaa tcgttttgga tggaatagca tgatggttgg cttttcatta gcgggtcttg
     1321 gtcttttaca ctcagtattc caagcctttg tggcaggaag aatagccact aaatggggcg
     1381 aaaaaacggc agtactgctc ggatttattg cagatagtag tgcatttgcc tttttagcgt
     1441 ttatatctga aggttggtta gttttccctg ttttaatttt attggctggt ggtgggatcg
     1501 ctttacctgc attacaggga gtgatgtcta tccaaacaaa gagtcatcag caaggtgctt
     1561 tacagggatt attggtgagc cttaccaatg caaccggtgt tattggccca ttactgtttg
     1621 ctgttattta taatcattca ctaccaattt gggatggctg gatttggatt attggtttag
     1681 cgttttactg tattattatc ctgctatcga tgaccttcat gttaacccct caagctcagg
     1741 ggagtaaaca ggagacaagt gcttagttat ttcgtcacca aatgatgtta ttccgcgaaa
     1801 tataatgacc ctcttgataa cccaagaggg cattttttac gataaagaag atttagcttc
     1861 aaataaaacc tatctatttt atttatcttt caagctcaat aaaaagccgc ggtaaatagc
     1921 aataaattgg ccttttttat cggcaagctc ttttaggttt ttcgcatgta ttgcgatatg
     1981 cataaaccag ccattgagta agtttttaag cacatcatca tcataagctt taagttggtt
     2041 ctcttggatc aatttgctga caatggcgtt taccttacca gtaatgtatt caaggctaat
     2101 tttttcaagt tcattccaac caatgatagg catcacttct tggataggga taaggttttt
     2161 attattatca ataatataat caagataatg ttcaaatata ctttctaagg cagaccaacc
     2221 atttgttaaa tcagtttttg ttgtgatgta ggcatcaatc ataattaatt gctgcttata
     2281 acaggcactg agtaattgtt ttttattttt aaagtgatga taaaaggcac ctttggtcac
     2341 caacgctttt cccgagatct catctattga aacagcttga tagccttttt caacaaacaa
     2401 tattcgtgct gagttaacca gtgattgata ggtactctta aaattttctt gttgatgatt
     2461 tttattttcc atgatagatt taaaataaca taccgtcagt atgtttatgg tatcatgatg
     2521 atgtggtcgt gacaatctta agaacattta ggttatttta tgtatgttga acagcattct
     2581 cgctatcaaa ataaagctaa taacat
//
LOCUS       DAMZFT010000439         2297 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.guided.303, whole genome shotgun sequence.
ACCESSION   DAMZFT010000439 DAMZFT010000000
VERSION     DAMZFT010000439.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2297)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2297)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2297
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.guided.303"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            <1..194
                     /gene="frdB"
                     /locus_tag="PN977_005498"
     CDS             <1..194
                     /gene="frdB"
                     /locus_tag="PN977_005498"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fumarate reductase iron-sulfur protein"
                     /protein_id="HDI6045366.1"
                     /translation="EDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKHVDPAAAI
                     QQGKVESSKDFLIATLKPR"
     gene            205..600
                     /gene="frdC"
                     /locus_tag="PN977_005499"
     CDS             205..600
                     /gene="frdC"
                     /locus_tag="PN977_005499"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HDI6045367.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FGLFALKNGPEAWAGFVDFLQNPVIVIINLITLAAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            611..970
                     /gene="frdD"
                     /locus_tag="PN977_005500"
     CDS             611..970
                     /gene="frdD"
                     /locus_tag="PN977_005500"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HDI6045368.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGVVTI"
     gene            1033..2166
                     /locus_tag="PN977_005501"
     CDS             1033..2166
                     /locus_tag="PN977_005501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HDI6045369.1"
                     /translation="MFKTTLCTLLITASCSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPTTKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMKTRVFQPLKLNHTWINVPSAEEKNYAWG
                     YREGKAVHVSPGALDAEAYGVKSTIEDMARWVQSNLKPFDINEKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDIIINGSDNKIALAARPVKPITPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVTAAWQILNTLQ"
BASE COUNT          575 a          582 c          583 g          557 t
ORIGIN      
        1 acgaagatag ccgcgaccac ggtaagaagg agcgtatggc gcagttgaac agccagaacg
       61 gcgtatggag ctgtactttc gtgggctact gctccgaagt ctgcccgaaa cacgtcgatc
      121 cggctgcggc cattcagcag ggcaaagtag aaagttcgaa agactttctt atcgcgaccc
      181 tgaaaccacg ctaaggagtg caacatgacg actaaacgta aaccgtatgt acggccaatg
      241 acgtccacct ggtggaaaaa attgccgttt tatcgctttt acatgctgcg cgaaggcacg
      301 gcggttccgg ctgtgtggtt cagcattgaa ctgattttcg ggctgtttgc cctgaaaaat
      361 ggcccggaag cctgggcggg attcgtcgac tttttacaaa acccggttat cgtgatcatt
      421 aacctgatca ctctggcggc agccctgctg cacaccaaaa cctggtttga gctggcacca
      481 aaagcagcca atatcattgt aaaagacgaa aaaatgggac cagagccaat tatcaaaagt
      541 ctctgggcgg taactgtggt tgccaccatc gtaatcctgt ttgttgccct gtactggtaa
      601 ggagcctgag atgattaatc caaatccaaa gcgttctgac gaaccggtat tctggggcct
      661 cttcggggcc ggtggtatgt ggagcgcaat tattgcgccg gtgatgatcc tgctggtggg
      721 tattctgctg ccactggggc tatttccggg tgatgcgctg agctacgagc gcgttctggc
      781 gttcgcgcag agcttcattg gtcgcgtatt cctgttcctg atgatcgttc tgccgctgtg
      841 gtgtggttta caccgtatgc accacgcgat gcacgatctg aaaatccacg tacctgcggg
      901 taaatgggtt ttctacggtc tggctgctat cctgacagtt gtcacactga ttggtgtcgt
      961 tacaatctaa cgcatcgcca atgtaaatcc ggcccgccta tggcgggccg ttttgtatgg
     1021 aaaccagacc ttatgttcaa aacgacgctc tgcaccttat taattaccgc ctcttgctcc
     1081 acatttgccg cccctcaaca aatcaacgat attgtgcatc gcacaattac cccgcttata
     1141 gagcaacaaa agatccccgg tatggcggtg gcggtaattt atcagggtaa accttattac
     1201 tttacctggg gctatgcgga catcgccaaa aagcagcccg tcacacagca aacgttgttt
     1261 gagttaggtt cggtcagcaa aacatttacg ggcgtgcttg gtggcgacgc tattgctcga
     1321 ggggaaatca agttaagcga tcccacaaca aaatactggc ctgaacttac cgctaaacag
     1381 tggaatggga tcacactatt acatctcgca acctacaccg ctggcggcct gccattgcag
     1441 gtgccggatg aggtgaaatc ctcaagcgac ttgctgcgct tctatcaaaa ctggcagcct
     1501 gcatgggctc caggaacaca acgtctgtat gccaactcca gtatcggttt gttcggcgca
     1561 ctggctgtga agccgtctgg tttgagtttt gagcaggcga tgaaaactcg tgtcttccag
     1621 ccactcaaac tcaatcatac gtggattaat gtaccgtccg cagaagaaaa gaactacgcc
     1681 tggggatatc gcgaaggtaa ggcagtacat gtttcgccag gggcgttaga tgctgaagct
     1741 tatggtgtga agtcgaccat tgaagatatg gcccgctggg tgcaaagcaa tttaaaaccc
     1801 tttgatatca atgagaaaac acttcaacaa gggatacaac tggcacaatc tcgctactgg
     1861 caaaccggcg atatgtatca gggtctgggc tgggaaatgc tggactggcc ggtaaatcct
     1921 gacatcatca ttaacggcag tgacaataaa attgcactgg cagcacgccc cgtaaaaccg
     1981 attacgcccc caactcctgc agtacgtgca tcatgggtac ataaaacggg ggcgaccggc
     2041 ggatttggta gctatgtcgc gtttattcca gaaaaagagc tgggtatcgt gatgctggct
     2101 aacaaaaact atcccaatcc agcgagagtt accgccgcct ggcagattct caacactcta
     2161 cagtaaaatt tcatcgggtc cgaattttcg gaccttttct ccgcttttcc ttgctgtcat
     2221 ctacacttag aaaaaaccag taaggaaaca tttatgcgcc tgctccctct cgttgccgca
     2281 gcgacagctg catttct
//
LOCUS       DAMZFT010000440         1096 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.guided.349, whole genome shotgun sequence.
ACCESSION   DAMZFT010000440 DAMZFT010000000
VERSION     DAMZFT010000440.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1096)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1096)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1096
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.guided.349"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            7..867
                     /gene="aac(3)-IId"
                     /locus_tag="PN977_005502"
     CDS             7..867
                     /gene="aac(3)-IId"
                     /locus_tag="PN977_005502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000557454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(3)-IId"
                     /protein_id="HDI6045370.1"
                     /translation="MHTRKAITEALQKLGVQTGDLLMVHASLKAIGPVEGGAETVVAA
                     LRSAVGPTGTVMGYASWDRSPYEETLNGARLDDEARRTWLPFDPATAGTYRGFGLLNQ
                     FLVQAPGARRSAHPDASMVAVGPLAETLTEPHELGHALGEGSPVERFVRLGGKALLLG
                     APLNSVTALHYAEAVADIPNKRWVTYEMPMLGRDGEVAWKTASDYDSNGILDCFAIEG
                     KPDAVETIANAYVKLGRHREGVVGFAQCYLFDAQDIVTFGVTYLEKHFGTTPIVPPHE
                     AVERSCEPSG"
     gene            880..>1096
                     /locus_tag="PN977_005503"
     CDS             880..>1096
                     /locus_tag="PN977_005503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tunicamycin resistance protein"
                     /protein_id="HDI6045371.1"
                     /translation="MIIWINGPFGAGKTTLAKRLRDRRSKSLIFDPEEIGFVVKETVP
                     MPASGDYQDLPLWRGLTIAAVREIRRNY"
BASE COUNT          224 a          302 c          348 g          222 t
ORIGIN      
        1 atcgcgatgc atacgcggaa ggcaataacg gaggcgcttc aaaaactcgg agtccaaacc
       61 ggtgacctct tgatggtgca tgcctcactt aaagcgattg gtccggtcga aggaggagcg
      121 gagacggtcg ttgccgcgtt acgctccgcg gttgggccga ctggcactgt gatgggatac
      181 gcgtcgtggg accgatcacc ctacgaggag actctgaatg gcgctcggct ggatgacgaa
      241 gcccgccgta cctggctgcc gttcgatccc gcaacagccg ggacttaccg tgggttcggc
      301 ctgctgaatc aatttctggt tcaagccccc ggcgcgcggc gcagcgcgca ccccgatgca
      361 tcgatggtcg cggttggtcc gctggctgaa acgctgacgg agcctcacga actcggtcac
      421 gccttggggg aaggatcgcc cgtcgagcgg ttcgttcgcc ttggcgggaa ggccctgctg
      481 ttgggtgcgc cgctaaactc cgttaccgca ttgcactacg ccgaggcggt tgccgatatc
      541 cccaacaaac ggtgggtgac gtatgagatg ccgatgcttg gaagagacgg tgaagtcgcc
      601 tggaaaacgg catcggatta cgattcaaac ggcattctcg attgctttgc tatcgaagga
      661 aagccggatg cggttgaaac tatagcaaat gcttacgtga agctcggtcg ccatcgagaa
      721 ggtgtcgtgg gctttgctca gtgctacctg ttcgacgcgc aggacatcgt gacgttcggc
      781 gtcacctatc ttgagaagca tttcggaacc actccgatcg tgcctccgca cgaggccgtc
      841 gagcgctctt gcgagccttc aggttagagg ccgtcgacaa tgataatctg gatcaacgga
      901 cctttcggcg ccggaaagac gacgctcgct aagcggctgc gcgatcggcg ttccaaatcg
      961 ctgatctttg accccgagga aatcgggttc gtggtgaaag aaacggtccc catgccagcg
     1021 agcggagact atcaggatct ccccttgtgg aggggactta cgatcgcggc ggtcagggag
     1081 attcgaagga attact
//
LOCUS       DAMZFT010000441          850 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.guided.364, whole genome shotgun sequence.
ACCESSION   DAMZFT010000441 DAMZFT010000000
VERSION     DAMZFT010000441.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 850)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 850)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..850
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.guided.364"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(<1..146)
                     /locus_tag="PN977_005504"
     CDS             complement(<1..146)
                     /locus_tag="PN977_005504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage integrase N-terminal SAM-like
                     domain-containing protein"
                     /protein_id="HDI6045372.1"
                     /translation="MKTATAPLPPLRSVKVLDQLRERIRYLHYSLRTEQTYVHWVRAF
                     IRFHG"
     gene            304..777
                     /locus_tag="PN977_005505"
     CDS             304..777
                     /locus_tag="PN977_005505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001389366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrA17"
                     /protein_id="HDI6045373.1"
                     /translation="MKISLISAVSENGVIGSGPDIPWSVKGEQLLFKALTYNQWLLVG
                     RKTFDSMGVLPNRKYAVVSKNGISSSNENVLVFPSIENALKELSKVTDHVYVSGGGQI
                     YNSLIEKADIIHLSTVHVEVEGDIKFPIMPENFNLVFEQFFMSNINYTYQIWKKG"
BASE COUNT          246 a          161 c          198 g          245 t
ORIGIN      
        1 ccgtggaaac ggatgaaggc acgaacccag tggacataag tctgttcggt tcgtaaactg
       61 taatgcaagt agcgtatgcg ctcacgcaac tggtccagaa ccttgaccga acgcagcggt
      121 ggtaacggcg cagtggcggt tttcatggct tgttatgact gtttttttgt acagtctatg
      181 cctcgggcat ccaagcagca agcgcgttac gccgtgggtc gatgtttgat gttatggagc
      241 agcaacgatg ttacgcagca gggcagtcgc cctaaaacaa agttagccat taagggagtt
      301 aaattgaaaa tatcattgat ttctgcagtg tcagaaaatg gcgtaatcgg tagtggtcct
      361 gatatcccgt ggtcagtaaa aggtgagcaa ctactcttta aagcgctcac atataatcaa
      421 tggctccttg tcggaagaaa aacatttgac tctatgggtg ttcttccaaa tcgcaaatat
      481 gcagtagtgt caaagaacgg aatttcaagc tcaaatgaaa acgtcctagt ttttccttca
      541 atagaaaatg ctttgaaaga gctatcaaaa gttacagatc atgtatatgt ctctggcggg
      601 ggtcaaatct ataatagcct tattgaaaaa gcagatataa ttcatttgtc tactgttcac
      661 gttgaagtcg aaggtgatat caaattccct ataatgcctg agaatttcaa tttggttttt
      721 gaacagtttt ttatgtctaa tataaattat acataccaga tttggaaaaa aggctaacaa
      781 tgcgttgcag caccagtcgc ttcgctcctt gggtgatgct gccaacttac tgatttagtg
      841 tatgatggtg
//
LOCUS       DAMZFT010000442          850 bp    DNA     linear   BCT 27-JAN-2023
DEFINITION  TPA_asm: Escherichia coli strain UMNRS2747
            SAMN32919534-rid16879923.guided.365, whole genome shotgun sequence.
ACCESSION   DAMZFT010000442 DAMZFT010000000
VERSION     DAMZFT010000442.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32919534
            Sequence Read Archive: SRR23232073
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 850)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 850)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001591567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001591567.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/26/2023 05:47:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,507
            CDSs (total)                      :: 5,444
            Genes (coding)                    :: 5,285
            CDSs (with protein)               :: 5,285
            Genes (RNA)                       :: 63
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 2, 3 (5S, 16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 85 of 159
            Pseudo Genes (incomplete)         :: 78 of 159
            Pseudo Genes (internal stop)      :: 44 of 159
            Pseudo Genes (multiple problems)  :: 39 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..850
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32919534-rid16879923.guided.365"
                     /strain="UMNRS2747"
                     /isolation_source="intestine"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2020"
                     /collected_by="Arm and Hammer"
     gene            complement(<1..146)
                     /locus_tag="PN977_005506"
     CDS             complement(<1..146)
                     /locus_tag="PN977_005506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage integrase N-terminal SAM-like
                     domain-containing protein"
                     /protein_id="HDI6045374.1"
                     /translation="MKTATAPLPPLRSVKVLDQLRERIRYLHYSLRTEQTYVHWVRAF
                     IRFHG"
     gene            304..777
                     /gene="dfrA"
                     /locus_tag="PN977_005507"
     CDS             304..777
                     /gene="dfrA"
                     /locus_tag="PN977_005507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001389366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrA"
                     /protein_id="HDI6045375.1"
                     /translation="MKISLISAVSENGVIGSGPDIPWSVKGEQLLFKALTYNQWLLVG
                     RKTFDSMGVLPNRKYAVVSKNGISSSNENVLVFPSIENALKELSKVTDHVYVSGGGQI
                     YNSLIEKADIIHLSTVHVEVEGYIKFPIMPENFNLVFEQFFMSNINYTYQIWKKG"
BASE COUNT          246 a          161 c          197 g          246 t
ORIGIN      
        1 ccgtggaaac ggatgaaggc acgaacccag tggacataag tctgttcggt tcgtaaactg
       61 taatgcaagt agcgtatgcg ctcacgcaac tggtccagaa ccttgaccga acgcagcggt
      121 ggtaacggcg cagtggcggt tttcatggct tgttatgact gtttttttgt acagtctatg
      181 cctcgggcat ccaagcagca agcgcgttac gccgtgggtc gatgtttgat gttatggagc
      241 agcaacgatg ttacgcagca gggcagtcgc cctaaaacaa agttagccat taagggagtt
      301 aaattgaaaa tatcattgat ttctgcagtg tcagaaaatg gcgtaatcgg tagtggtcct
      361 gatatcccgt ggtcagtaaa aggtgagcaa ctactcttta aagcgctcac atataatcaa
      421 tggctccttg tcggaagaaa aacatttgac tctatgggtg ttcttccaaa tcgcaaatat
      481 gcagtagtgt caaagaacgg aatttcaagc tcaaatgaaa acgtcctagt ttttccttca
      541 atagaaaatg ctttgaaaga gctatcaaaa gttacagatc atgtatatgt ctctggcggg
      601 ggtcaaatct ataatagcct tattgaaaaa gcagatataa ttcatttgtc tactgttcac
      661 gttgaagtcg aaggttatat caaattccct ataatgcctg agaatttcaa tttggttttt
      721 gaacagtttt ttatgtctaa tataaattat acataccaga tttggaaaaa aggctaacaa
      781 tgcgttgcag caccagtcgc ttcgctcctt gggtgatgct gccaacttac tgatttagtg
      841 tatgatggtg
//
